cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0t43s38
title: CIL:35795, Saccharomyces cerevisiae. In Cell Image Library

description: The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Read the microscopy section of the referenced paper for details prior to image analysis.
description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Bck2, a Cln-independent activator of CLN1,2 expression. These phase and GFP images are part of a group that ranges from CIL:35780-35799. Note that there are additional groups of images monitoring the dynamics of other proteins involved in cell cycle regulation by the same authors in the Library.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0w95835
title: CIL:35460, Cricetulus griseus, ovary. In Cell Image Library

description: Chinese Hamster Ovary (CHO) cells were metaphase-arrested with colcemid, exposed to hypotonic salt to swell the cells and chromosomes, fixed, embedded in plastic, and sections observed by high voltage electron microscopy (HVEM). The chromatid pair seen in the image was recorded at a specimen tilt angle of 30 degrees. Grouped with this image are tilts of 40 and 50 degrees, providing oblique stereo views of the chromatids.
description: Metaphase arrested cells were swollen in 0.07 M KCl, fixed with 10% paraformaldehyde, post-fixed with OsO4, dehydrated, embedded in plastic, and 0.25 micron sections obtained. Sections were observed with the Wisconsin HVEM operated at 1 MeV, and micrographs taken at three tilt angles. See Ris, H (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
description: Preparation: formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j0rj4hfb
title: CIL:35552, Cricetulus griseus, ovary. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. Seen is a grazing section of chromosomes showing kinetochores. This image was recorded with a specimen tilt of 55 degrees. Grouped with it is one recorded at 45 degrees, providing an oblique stereo view.
description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
DOI: 10.6075/j01z4396
title: CIL:35554, Cricetulus griseus, ovary. In Cell Image Library

description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. This image was recorded with a specimen tilt of 55 degrees. Grouped with it is one recorded at 45 degrees, providing an oblique stereo view of chromosome organization.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0zc81vk
title: CIL:35551, Cricetulus griseus, ovary. In Cell Image Library

description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. Seen is a grazing section of chromosomes showing kinetochores. This image was recorded with a specimen tilt of 45 degrees. Grouped with it is one recorded at 55 degrees, providing an oblique stereo view of chromosome organization.
description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0ww7gkj
title: CIL:35562, Cricetulus griseus, ovary. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. Seen is a longitudinal section through a chromatid pair with kinetochore elements. This image recorded with a specimen tilt of 20 degrees is part of a grouped tilt series ranging from 20 degrees to 75 degrees. Pairs providing oblique stereo views of chromosome organization.
description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
DOI: 10.6075/j0m61j5b
title: CIL:35459, Cricetulus griseus, ovary. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Chinese Hamster Ovary (CHO) cells were metaphase-arrested with colcemid, exposed to hypotonic salt to swell the cells and chromosomes, fixed, embedded in plastic, and sections observed by high voltage electron microscopy (HVEM). The chromatid pair seen in the image was recorded at a specimen tilt angle of 60 degrees. Grouped with this image are tilts of 41, 50 and 70 degrees, providing oblique stereo views of the chromatids.
description: Metaphase arrested cells were swollen in 0.07 M KCl, fixed with 10% paraformaldehyde, post-fixed with OsO4, dehydrated, embedded in plastic, and 0.25 micron sections obtained. Sections were observed with the Wisconsin HVEM operated at 1 MeV, and micrographs taken at four tilt angles. See Ris, H (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
DOI: 10.6075/j0pn94mb
title: CIL:35456, Cricetulus griseus, ovary. In Cell Image Library

description: Chinese Hamster Ovary (CHO) cells were metaphase-arrested with colcemid, exposed to hypotonic salt to swell the cells and chromosomes, fixed, embedded in plastic, and sections observed by high voltage electron microscopy (HVEM). The chromatid pair seen in the image was recorded at a specimen tilt angle of 40 degrees. Grouped with this image are tilts of 30 and 50 degrees, providing oblique stereo views of the chromatids.
description: Metaphase arrested cells were swollen in 0.07 M KCl, fixed with 10% paraformaldehyde, post-fixed with OsO4, dehydrated, embedded in plastic, and 0.25 micron sections obtained. Sections were observed with the Wisconsin HVEM operated at 1 MeV, and micrographs taken at three tilt angles. See Ris, H (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
description: Preparation: formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j0g73ckh
title: CIL:35455, Cricetulus griseus, ovary. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Chinese Hamster Ovary (CHO) cells were metaphase-arrested with colcemid, exposed to hypotonic salt to swell the cells and chromosomes, fixed, embedded in plastic, and sections observed by high voltage electron microscopy (HVEM). The chromatid pair seen in the image was recorded at a specimen tilt angle of 30 degrees. Grouped with this image are tilts of 40 and 50 degrees, providing oblique stereo views of the chromatids.
description: Metaphase arrested cells were swollen in 0.07 M KCl, fixed with 10% paraformaldehyde, post-fixed with OsO4, dehydrated, embedded in plastic, and 0.25 micron sections obtained. Sections were observed with the Wisconsin HVEM operated at 1 MeV, and micrographs taken at three tilt angles. See Ris, H (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
DOI: 10.6075/j0bk1b9z
title: CIL:35483, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; a print was scanned for Photoshop
Data qualification: Processed
description: An image of the oral ribs with their microtubular ribbons and alveolar tubes pass through the cytostome to the cytopharynx and nascent vacuole region. In this view the left wall of the buccal cavity is in grazing section so the partial layer of microtubules appears on both sides of the cytostome. The only membrane not associated with alveoli is that at the beginning of the cytopharynx. TEM taken on 8/29/67 by R. Allen with Philips 200 operating at 60kV. Neg. 25,700X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:34847) is available for quantitative analysis.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j05m64p3
title: CIL:35464, Rattus norvegicus, blood vessel endothelial cell. In Cell Image Library

description: Hitachi Field Emission SEM model S-4700.
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: scanning electron microscopy (SEM)
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: secondary electron generation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Raw;spatialmeasurements
description: Liver sinusoid of a Brown Rat with fenestrated endothelial cells. Sinusoidal width is about 5 microns. Original magnification is 10,000x . Note the microvilli of hepatocytes in the space of Disse external to the endothelium.
DOI: 10.6075/j0z03722
title: CIL:35463, Rattus norvegicus, blood vessel endothelial cell. In Cell Image Library

description: Phase contrast microscopy of liver sinusoidal endothelial cells purified from a Brown Rat.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: phase contrast microscopy
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Data qualification: Raw;spatialmeasurements
description: Nikon eclipse Ti-S with a 20x objective.
DOI: 10.6075/j09k4944
title: CIL:35462, Rattus norvegicus, blood vessel endothelial cell. In Cell Image Library

description: Phase contrast microscopy of purified liver sinusodial endothelial cells (LSECs) from Brown Rat.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: phase contrast microscopy
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Data qualification: Raw;spatialmeasurements
description: Nikon Eclipse Ti-S with a 20x objective.
DOI: 10.6075/j0ww7gm0
title: CIL:35548, Cricetulus griseus, ovary. In Cell Image Library

description: Metaphase-arrested cells were fixed with 3:1 ethanol:acetic acid, transferred to 50% acetic acid, and passed though a syringe to break cells. Drops of sample were placed on formvar coated grids, stained with uranly acetate, and critical point dried. Grids were observed with the Wisconsin HVEM at 1 MeV. See Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth. Cell Biol 18:229-246.
description: CHO cells were arrested in metaphase with colcemid, swollen in 75mM KCl, fixed, disrupted, and observed using High Voltage EM. This image was recorded with a specimen tilt angle of 46 degrees. Grouped with it is an image of the same area at a tilt angle of 54 degrees, providing an oblique stereo view of chromosome organization.
description: Preparation: coagulating-fixative fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j0c53jr6
title: CIL:35549, Cricetulus griseus, ovary. In Cell Image Library

description: Preparation: coagulating-fixative fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: CHO cells were arrested in metaphase with colcemid, swollen in 75mM KCl, fixed, disrupted, and observed using High Voltage EM. This image was recorded with a specimen tilt angle of 46 degrees. Grouped with it is an image of the same area at a tilt angle of 54 degrees, providing an oblique stereo view of chromosome organization.
description: Metaphase-arrested cells were fixed with 3:1 ethanol:acetic acid, transferred to 50% acetic acid, and passed though a syringe to break cells. Drops of sample were placed on formvar coated grids, stained with uranly acetate, and critical point dried. Grids were observed with the Wisconsin HVEM at 1 MeV. See Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth. Cell Biol 18:229-246.
DOI: 10.6075/j0sj1jht
title: CIL:35547, Cricetulus griseus, ovary. In Cell Image Library

description: Preparation: coagulating-fixative fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Metaphase-arrested cells were fixed with 3:1 ethanol:acetic acid, transferred to 50% acetic acid, and passed though a syringe to break cells. Drops of sample were placed on formvar coated grids, stained with uranly acetate, and critical point dried. Grids were observed with the Wisconsin HVEM at 1 MeV. See Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth. Cell Biol 18:229-246.
description: CHO cells were arrested in metaphase with colcemid, swollen in 75mM KCl, fixed, disrupted, and observed using High Voltage EM. This image was recorded with a specimen tilt angle of 54 degrees. Grouped with it is an image of the same area at a tilt angle of 46 degrees, providing an oblique stereo view of chromosome organization.
DOI: 10.6075/j0cr5sbb
title: CIL:35546, Cricetulus griseus, ovary. In Cell Image Library

description: CHO cells were arrested in metaphase with colcemid, swollen in 75mM KCl, fixed, disrupted, and observed using High Voltage EM. This image was recorded with a specimen tilt angle of 46 degrees. Grouped with it is an image of the same area at a tilt angle of 54 degrees, providing an oblique stereo view of chromosome organization.
description: Metaphase-arrested cells were fixed with 3:1 ethanol:acetic acid, transferred to 50% acetic acid, and passed though a syringe to break cells. Drops of sample were placed on formvar coated grids, stained with uranly acetate, and critical point dried. Grids were observed with the Wisconsin HVEM at 1 MeV. See Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth. Cell Biol 18:229-246.
description: Preparation: coagulating-fixative fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j0th8km6
title: CIL:35557, Cricetulus griseus, ovary. In Cell Image Library

description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. Seen is a longitudinal section through a chromatid pair with kinetochore elements. This image recorded with a specimen tilt of 35 degrees is part of a grouped tilt series ranging from 20 degrees to 75 degrees. Pairs providing oblique stereo views of chromosome organization.
description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0h41qcg
title: CIL:35555, Cricetulus griseus, ovary. In Cell Image Library

description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. This image was recorded with a specimen tilt of 45 degrees. Grouped with it is one recorded at 55 degrees, providing an oblique stereo view of chromosome organization.
description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0dn440w
title: CIL:35553, Cricetulus griseus, ovary. In Cell Image Library

description: Colcemid-arrested cells were swollen in hypotonic 75mM KCl, fixed with 4% HCOH, post-fixed with OsO4, embedded in plastic, and 'thick' 0.25 micron sections prepared. Sections were recorded using the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Metaphase-arrested CHO cells were swollen in 75 mM KCL, fixed with formaldehyde, post-fixed with OsO4, embedded, and 0.25 micron sections examined by High Voltage EM. This image was recorded with a specimen tilt of 45 degrees. Grouped with it is one recorded at 55 degrees, providing an oblique stereo view of chromosome organization.