cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14305504
title: Utilization and fate of phosphorus of different sources applied to cohesive soil of Amazonian periphery

description: ABSTRACT In the Amazonian region, P is often a primary factor limiting sustainable agrosystems. We compared the efficiencies of local aluminous phosphate (ALP) and single superphosphate (SSP) under a cover of leguminous residues to determine the fate of P sources in an Amazonian soil with hardsetting characteristics. The experiment followed a randomized block design with four replications and the following treatments: ALPU, i.e. ALP plus urea (U); ALPL, i.e., ALP plus leucaena (L); SSPU, i.e., SSP plus urea; SSPL, i.e., SSP plus leucaena; and BS, i.e., bare soil (without residues). To assess the residual values of the P sources, we used a sequence of crops consisting of maize, cowpea and cassava. Both ALP and SSP exhibited low P efficiency in BS. The greatest amounts of P and N uptake in the plots where P sources and leucaena residues were added, as in those covered with leucaena residue, resulted in higher productivity levels. These differences are important for the adoption of beneficial soil management practices and the use of P sources to enhance efficiency in tropical soils. The use of residues increased the P use efficiency of both P sources, as it enhanced the uptake of both N and soluble P. The replacement of SSP with ALP may be advantageous in the second year of planting with high-demand crops, but the P of the SSP retained in the minus soluble fractions may be available if the SSP is used in P-depleting crops combined with no-tillage underneath a mulch of residues.
DOI: 10.6084/m9.figshare.14305502
title: Performance of Holstein calves having free access to milk and dosed with Megasphaera elsdenii

description: ABSTRACT Megasphaera elsdenii converts lactate and glucose into butyrate, the main volatile fatty acid responsible of papillae development and may benefit calf performance. Twenty-six Holstein calves (BW = 34.5 ± 1.65 kg) were randomly assigned at birth to a control group (Meg0) and a group that received an oral dose of M. elsdenii NCIMB 41125 at 14 d of age (Meg14). Calves received colostrum for the first 3 d followed by free choice access to whole milk until weaning at 56 d. From d 4 onward, starter and water were offered ad libitum. Intakes were measured daily and body weights (BW) weekly. Blood samples were collected on day 7, 21, 28, 42, and 56 for β-hydroxybutyrate (BHBA) analysis. Performance was measured for an additional 14 d post-weaning. Pre-weaning milk intake was lower (p = 0.010) and starter DMI (dry matter intake) greater (p = 0.001) for Meg14 than Meg0 calves. Total DMI, metabolisable energy (ME) intake and average daily gain (ADG) were similar (p &gt; 0.05) for both groups, but Meg14 calves had greater weaning BW (p = 0.012) and feed efficiency (p &lt; 0.029). The average BHBA between d 21 and 56 was greater for Meg14 (p = 0.03) compared to Meg0 calves. After weaning, Meg14 calves had greater DMI (p = 0.027), ME intake (p = 0.023) and ADG (p = 0.002) and tended to have better feed efficiencies (p = 0.07) than Meg0 calves. Administering M. elsdenii NCIMB 41125 improved starter intake and feed efficiency, which was associated with high blood BHBA.
DOI: 10.6084/m9.figshare.14305500
title: Identification and validation of novel EST-SSR markers in olives

description: ABSTRACT: The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) markers, obtained from 577,025 expressed sequence tags (ESTs) in developing olive fruits generated in the framework of the Slovenian national olive transcriptome project. Sequences were de novo assembled into 98,924 unigenes, which were then used as a source for microsatellites searching. We identified 923 unigenes that contained 984 SSRs among which dinucleotide SSRs (36 %) were the most abundant, followed by tri- (33 %) and hexa- (21 %) nucleotides. Microsatellite repeat motif GA (37 %) was the most common among dinucleotides, while microsatellite repeat motif GAA was the most abundant trinucleotide SSR motif (16 %). Gene ontology annotations could be assigned to 27 % of the unigenes. A hundred and ten expressed sequence tag-derived-simple sequence repeats (EST-SSRs) with annotated genes were selected for primer designing and finally, 46 (42 %) polymorphic EST-SSRs were successfully amplified and used to validate genetic diversity among 24 olive varieties. The average number of alleles per locus, observed heterozygosity, expected heterozygosity, and polymorphic information content were 4.5, 0.649, 0.604 and 0.539, respectively. Twenty-seven EST-SSRs showed good diversity properties and were recommended for further olive genome investigation.
DOI: 10.6084/m9.figshare.14305499
title: Biology and nutrition of Spodoptera frugiperda (Lepidoptera: Noctuidae) fed on different food sources

description: ABSTRACT: We studied Spodoptera frugiperda development using different food sources in the laboratory and field. Newly hatched larvae were fed soybean, cotton, maize, wheat, and oat leaves. An artificial diet was used as the control. Duration of pre-pupal, pupal, and larva-adult period, pupal weight, sex ratio, survival, larva feeding preferences, oviposition preferences, and nutritional quality of different hosts were evaluated. Insects fed on wheat showed the shortest larva-adult period. The insects fed on cotton and soybean had longer larval development cycles and pupae of lower weight. Feeding preference was evident for third instar larvae and did not differ between wheat, oat, maize, and soybean, which were the preferred hosts. Moths oviposited to a greater extent on the upper canopy of wheat than that of other plants in both the no-choice and free-choice tests. Treatments influenced insect growth, food consumption, and digestion when nutritional variables were analyzed. Thus, grasses were better hosts for S. frugiperda development. Cotton was the least preferred food, followed by soybean. The present study can improve our understanding of S. frugiperda in these different crops and help in developing management strategies. Even though S. frugiperda is considered to be polyphagous, this pest is closely associated with grasses (maize, wheat, oat) and has lower potential as a soybean or cotton feeder. Howerver, S. frugiperda food intake regulation appears to be triggered by a complex of different mechanisms. Thus, S. frugiperda can also damage soybean and cotton and adapt to them in the absence of preferred hosts.
DOI: 10.6084/m9.figshare.14305497
title: Enzyme complex supplementation in different nutrient levels diets on pigs feces excretion and anaerobic digestion

description: ABSTRACT This research aimed to evaluate enzyme supplementation in diets with different nutritional levels for pigs in their feces excretion and anaerobic digestion. Fifty four gilts were placed on diets formulated to achieve 100 or 95 or 90 % of a pig's nutrient requirements, with the addition of an enzyme complex containing amylase, β-glucanase, cellulase, pectinase, xylanase, protease and phytase (diets 100E, 95E and 90E) or without the enzymes (diets 100, 95 and 90). The trial was divided into three periods (1, 1-2 and 1-3) based on the animal weight. The enzyme complex reduced (p &lt; 0.05) excretions per period and per unit of weight gain (residue coefficients) of organic matter (OM) in Period 1, of N, Ca, Zn and Na in Period 1-2, and of Na excretions and residue coefficients in Period 1-3. Animals fed diet 95E had lower excretions (p &lt; 0.05) of dry (DM), organic and mineral matters, N, Ca, Fe, Mn and Cu, and lower residue coefficients (p &lt; 0.05) of DM, OM, P, Fe, Mn and Cu compared with the animals fed diet 95, in Period 1-3. Numerically, the enzyme complex improved biogas and methane production potentials in anaerobic digestion of the feces. The use of an enzyme complex in a diet formulated to provide 95 % of the gilts' nutrient requirements reduced fecal excretion and positively affected the anaerobic digestion of feces. These data show that pig nutrition and feeding are important tools for reducing the potential environmental impact of pig production.
DOI: 10.6084/m9.figshare.14305495
title: Energy balance and evapotranspiration of melon grown with plastic mulch in the Brazilian semiarid region

description: Melon plants (Cucumis melo L.) are grown in the state of Rio Grande do Norte (RN), the largest producer of melons in Brazil, with plastic mulch and agrotextiles. Studies of crop evapotranspiration (ET) under these conditions are required to ensure adequate irrigation. This study aimed to determine the crop coefficients (Kc) of irrigated melon plants grown with mulch and agrotextiles in the region of Mossoró, RN, based on the Bowen Ratio Energy Balance (BREB) method. Two experiments were conducted at different times during the 2009/2010 season in a melon producing area (4°59'52" S, 37°23'09" W, and 54 m elevation) to define ET and Kc. Due to the plastic cover and reduced precipitation during the experiments, the Kc obtained by the BREB method was considered the basal Kc - KcbBREB. The results were compared with the Kcb from the FAO 56 Bulletin. There was close agreement between BREB and FAO ET measurements (12 % underestimation by the FAO method for the entire crop season), with sizeable differences only during the initial phenological stage. The mean KcbBREB values of the two field campaigns were 0.26, 0.96 and 0.63 for the initial, midseason and late stages, respectively. The high KcbBREB value in the initial growth phase may be related to the effect of the plastic mulch and agrotextiles on the energy balance at the surface. The relationship between KcbBREB and fc had high correlation, making possible an estimation of the melon Kcb based on the level of crop ground cover.
DOI: 10.6084/m9.figshare.14305493
title: Quantitative genetics theory for genomic selection and efficiency of breeding value prediction in open-pollinated populations

description: ABSTRACT To date, the quantitative genetics theory for genomic selection has focused mainly on the relationship between marker and additive variances assuming one marker and one quantitative trait locus (QTL). This study extends the quantitative genetics theory to genomic selection in order to prove that prediction of breeding values based on thousands of single nucleotide polymorphisms (SNPs) depends on linkage disequilibrium (LD) between markers and QTLs, assuming dominance. We also assessed the efficiency of genomic selection in relation to phenotypic selection, assuming mass selection in an open-pollinated population, all QTLs of lower effect, and reduced sample size, based on simulated data. We show that the average effect of a SNP substitution is proportional to LD measure and to average effect of a gene substitution for each QTL that is in LD with the marker. Weighted (by SNP frequencies) and unweighted breeding value predictors have the same accuracy. Efficiency of genomic selection in relation to phenotypic selection is inversely proportional to heritability. Accuracy of breeding value prediction is not affected by the dominance degree and the method of analysis, however, it is influenced by LD extent and magnitude of additive variance. The increase in the number of markers asymptotically improved accuracy of breeding value prediction. The decrease in the sample size from 500 to 200 did not reduce considerably accuracy of breeding value prediction.
DOI: 10.6084/m9.figshare.14305491
title: Genome association study through nonlinear mixed models revealed new candidate genes for pig growth curves

description: ABSTRACT: Genome association analyses have been successful in identifying quantitative trait loci (QTLs) for pig body weights measured at a single age. However, when considering the whole weight trajectories over time in the context of genome association analyses, it is important to look at the markers that affect growth curve parameters. The easiest way to consider them is via the two-step method, in which the growth curve parameters and marker effects are estimated separately, thereby resulting in a reduction of the statistical power and the precision of estimates. One efficient solution is to adopt nonlinear mixed models (NMM), which enables a joint modeling of the individual growth curves and marker effects. Our aim was to propose a genome association analysis for growth curves in pigs based on NMM as well as to compare it with the traditional two-step method. In addition, we also aimed to identify the nearest candidate genes related to significant SNP (single nucleotide polymorphism) markers. The NMM presented a higher number of significant SNPs for adult weight (A) and maturity rate (K), and provided a direct way to test SNP significance simultaneously for both the A and K parameters. Furthermore, all significant SNPs from the two-step method were also reported in the NMM analysis. The ontology of the three candidate genes (SH3BGRL2, MAPK14, and MYL9) derived from significant SNPs (simultaneously affecting A and K) allows us to make inferences with regards to their contribution to the pig growth process in the population studied.
DOI: 10.5281/zenodo.4636983
title: Supplementary File S2. Specimen-level database.

description: Eggs and egg-sacs in pholcid spiders. Specimen level dataset.
DOI: 10.5281/zenodo.4636982
title: Supplementary File S2. Specimen-level database.

description: Eggs and egg-sacs in pholcid spiders. Specimen level dataset.
DOI: 10.6084/m9.figshare.14305489
title: Biogas production: litter from broilers receiving direct-fed microbials and an enzyme blend

description: ABSTRACT The effect of additives used in the feed of broilers on anaerobic bio-digestion of poultry litter was evaluated. Four diets were used: NC: negative control; DFM: NC + 500 ppm direct-fed microbials (DFM) containing Bacillus subtilis and Bacillus licheniformis; ENZ: diet formulated with an enzyme blend (20 ppm phytase, 200 ppm protease and 200 ppm xylanase); DFM+E: ENZ + DFM. Substrates for the anaerobic bio-digestion were prepared with litter from each treatment, containing 4 % total solids (TS). These were used in 16 continuous bio-digesters with a 2 kg d−1 load, to determine the production and potential biogas production and composition during an 85-day period. Influent and effluent samples were collected for the amounts of TS and volatile solids (VS), fiber fraction (neutral detergent fiber [NDF], acid detergent fiber [ADF] and lignin), nutrients (N, P and K), and total and thermotolerant coliforms to be determined. For all treatments a reduction in the following effluents was observed as follows: TS (49, 48, 48 and 50 %) VS (70, 54, 55 and 62 %) NDF (91, 90, 95 and 96 %) ADF (89, 88, 93 and 94 %) and lignin (80, 76, 89 and 88 %). The efficiency of the treatment for coliforms in bio-digesters was higher than 90 % in the 85-day period in all treatment groups. There was a reduction in biogas and methane production when DFM (5500 and 4000 mL) and DFM + E (5800 and 4100 mL) were used, compared to treatments NC (6300 mL and 4400) and ENZ (6400 and 4500 mL). The potential production of reduced TS and VS was higher in ENZ (1:00 and 1.74 106 mL kg−1) when compared to NC (0.88 and 1:02 106 mL kg−1), DFM (0.80 and 1:40 106 mL kg−1) and DFM + E (0.88 1:25 and 106 mL kg−1). The additives did not affect the percentage of methane production, and all treatments showed values higher than 70 %. Adding enzymes to the diet of broilers influences the litter characteristics and, as a consequence, increases biogas production. The addition of DFM and DFM + E to broiler diets reduced biogas and methane production.
DOI: 10.6084/m9.figshare.14305487
title: Resistance mechanisms of sugarcane cultivars to spittlebug Mahanarva fimbriolata

description: ABSTRACT The spittlebug Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) is one of the most important pests of sugarcane in Brazil. Measures for population control are currently restricted to the use of chemical insecticides and fungus Metarhizium anisopliae, in part because very little information exists regarding resistance of sugarcane cultivars. Therefore, the aim of this study was to evaluate resistance mechanisms of 12 different sugarcane cultivars to M. fimbriolata to provide information for growers for pest management. Isolated buds of each cultivar were planted in pots and kept in a greenhouse for approximately three months. The pots were then moved to climate test chambers (26 ± 1 ºC; 70 ± 10 % relative humidity; 12 h photoperiod) to carry out laboratory tests to evaluate adult feeding and female oviposition preferences (using both free-choice and no-choice tests) as well as the effects of cultivars on nymph development and cultivars tolerance to pest attack. The least attractive cultivars for adult feeding were IACSP96-2042 and IAC91-1099. Cultivars IACSP96-2042, IACSP96-3060 and IACSP94-2101 received the fewest eggs in the free-choice and no-choice oviposition tests and exhibited some level of antixenosis resistance. Cultivar IAC91-1099 showed the highest level of antibiosis resistance with a nymph survival rate in the roots of only 20 %. Finally, cultivar IACSP94-2094 appears to be tolerant to M. fimbriolata, as it did not show significant reductions in aboveground biomass weight, despite showing reduced leaf chlorophyll levels following pest attack.
DOI: 10.6084/m9.figshare.14305484
title: Humic substances and its distribution in coffee crop under cover crops and weed control methods

description: ABSTRACT Humic substances (HS) comprise the passive element in soil organic matter (SOM), and represent one of the soil carbon pools which may be altered by different cover crops and weed control methods. This study aimed to assess HS distribution and characteristics in an experimental coffee crop area subjected to cover crops and cultural, mechanical, and chemical weed control. The study was carried out at Londrina, in the state of Paraná, southern Brazil (23°21’30” S; 51°10’17” W). In 2008, seven weed control/cover crops were established in a randomized block design between two coffee rows as the main-plot factor per plot and soil sampling depths (0-10 cm, 10-20 cm, 20-30 cm and 30-40 cm) as a split-plot. HS were extracted through alkaline and acid solutions and analyzed by chromic acid wet oxidation and UV-Vis spectroscopy. Chemical attributes presented variations in the topsoil between the field conditions analyzed. Cover crop cutting and coffee tree pruning residues left on the soil surface may have interfered in nutrient cycling and the humification process. Data showed that humic substances comprised about 50 % of SOM. Although different cover crops and weed control methods did not alter humic and fulvic acid carbon content, a possible incidence of condensed aromatic structures at depth increments in fulvic acids was observed, leading to an average decrease of 53 % in the E4/E6 ratio. Humin carbon content increased 25 % in the topsoil, particularly under crop weed-control methods, probably due to high incorporation of recalcitrant structures from coffee tree pruning residues and cover crops.
DOI: 10.6084/m9.figshare.14305483
title: Evaluation of natural preservatives in combination with acid whey for use in fermented sausage

description: ABSTRACT Natural antioxidant and antimicrobial systems are set to become an important component in food preservation methodology. The effect of alternative natural preservatives (Sinapis alba L.-M, Rosmarinus officinalis L.-R, Juniperus communis L.-J) in combination with acid whey (AW) was investigated after the ripening period (21d) and over a prolonged storage period of sausage. An antioxidant activity of extracts exercise was performed. The antimicrobial, oxidative stability and sensory properties of these natural preservatives were compared to curing-control (C). Significantly lower rancid odor and rancid flavor (1.48) were observed for R and M compared with the C sample. The low level of these attributes evidenced the antioxidant properties of the R sample, which confirmed the lower CD (~0.48-0.48 µmol mg−1) and TBARS values (~0.41-1.02 mg MDA kg−1) during sausage storing and the highest antioxidant activity against ABTS+ radicals (~85.45 %). Incorporation of acid whey with rosemary extract will give the product a threefold effect: high quality (sensory acceptance), healthy benefit (elimination of nitrite and nitrate from meat products) and safety (improved microbiological and oxidative stability).
DOI: 10.6084/m9.figshare.14305481
title: Influence of animal fat substitution by vegetal fat on Mortadella-type products formulated with different hydrocolloids

description: Meat has played a crucial role in human evolution and is an important component of a healthy and well-balanced diet on account of its nutritional properties, its high biological value as a source of protein, and the vitamins and minerals it supplies. We studied the effects of animal fat reduction and substitution by hydrogenated vegetal fat, sodium alginate and guar gum. Fatty acid composition, lipid oxidation, color and instrumental texture as well as the sensorial difference between low, substituted-fat and the traditional formulations for mortadella-type products were analyzed. Both substitution and reduction of animal fat decreased the saturated fatty acids percentage from 40% down to 31%. A texture profile analysis showed differences between the formulations. Furthermore, lipid oxidation values were not significant for treatments as regards the type and quantity of fat used while the use of sodium alginate and guar gum reduced the amounts of liquid released after cooking. Animal fat substitution does cause, however, a difference in overall sensorial perception compared with non-substituted products. The results confirm the viability of substituting vegetal fat for animal fat.
DOI: 10.6084/m9.figshare.14305480
title: Auxinic herbicides, mechanisms of action, and weed resistance: A look into recent plant science advances

description: Auxin governs dynamic cellular processes involved at several stages of plant growth and development. In this review, we discuss the mechanisms employed by auxin in light of recent scientific advances, with a focus on synthetic auxins as herbicides and synthetic auxin resistance mechanisms. Two auxin receptors were reported. The plasma membrane receptor ABP1 (Auxin Binding Protein 1) alters the structure and arrangement of actin filaments and microtubules, leading to plant epinasty and reducing peroxisomes and mitochondria mobility in the cell environment. The second auxin receptor is the gene transcription pathway regulated by the SCFTir/AFB ubiquitination complex, which destroys transcription repressor proteins that interrupt Auxin Response Factor (ARF) activation. As a result mRNA related with Abscisic Acid (ABA) and ethylene are transcribed, producing high quantities of theses hormones. Their associated action leads to high production of Reactive Oxygen Species (ROS), leading to tissue and plant death. Recently, another ubiquitination pathway which is described as a new auxin signaling route is the F-box protein S-Phase Kinase-Associated Protein 2A (SKP2A). It is active in cell division regulation and there is evidence that auxin herbicides can deregulate the SKP2A pathway, which leads to severe defects in plant development. In this discussion, we propose that SFCSKP2A auxin binding site alteration could be a new auxinic herbicide resistance mechanism, a concept which may contribute to the current progress in plant biology in its quest to clarify the many questions that still surround auxin herbicide mechanisms of action and the mechanisms of weed resistance.