DOI: 10.5061/dryad.rv15dv4n7
title: Data from: Caloric restriction-mediated reproductive lifespan extension across multiple strains of the clonal aquatic plant <em>Lemna turionifera</em>

description: Lifespan extension due to caloric restriction (CR) is a well-established
 aspect of animal senescence that has been observed in many taxa.
 Contrastingly, there is much less evidence in plants, even though it is
 straightforward to manipulate CR by restricting photosynthesis through
 reduction in light intensity. One of the few studies to report CR-mediated
 plant lifespan extension investigated reproductive lifespan in a single
 strain of the duckweed Lemna minor, a tiny, floating, aquatic plant. Here,
 with an aim of beginning to test the generality of this phenomenon in
 plants, we considered a congeneric species, L. turionifera, and examined
 CR-mediated lifespan extension in eight strains collected from Alberta,
 Canada. We grew plants in the lab under axenic conditions, and manipulated
 light intensity (and hence, putatively, CR) with neutral density filters.
 Plants that grew under dimmer conditions had longer reproductive
 lifespans, on average, than those that grew under brighter conditions,
 consistent with CR-mediated lifespan extension. However, this came at the
 expense of a reduction in the capacity to contribute to population growth:
 plants in dimmer conditions produced about the same total offspring spread
 across their longer lifespans, leading to a reduced intrinsic rate of
 increase, measured at the level of the individual. Expanding the taxonomic
 scope of studies on CR-mediated lifespan extension—especially in
 plants—remains an important goal in senescence research.
description: # Data from: Caloric restriction-mediated reproductive lifespan extension
 across multiple strains of the clonal aquatic plant *Lemna turionifera*
 Dataset DOI:
 [10.5061/dryad.rv15dv4n7](https://doi.org/10.5061/dryad.rv15dv4n7) ##
 Description of the data and file structure There are two data files (.csv)
 and one code file (.r):
 "CR_strains_daily_reproduction_2025_03_08.csv" contains daily
 reproduction data for *Lemna turionifera* plants in our experiment
 "CR_strains_temperature_2025_03_08.csv" contains temperature
 data for our experimental setup under bright light and dim light
 conditions "CR_strains_analysis_2025_11_21.r" contains R code
 for data analysis ### Files and variables #### File:
 CR_strains_daily_reproduction_2025_03_08.csv **Description:** Data file
 associated with our daily reproduction observations (see main text in our
 article). It has 112 rows (not including the header row) and 76 columns.
 ##### Variables * id: ID number of the experimental subject. * strain: The
 *Lemna turionifera* strain to which the experimental subject belonged
 (format: Anz = Anz C = Anzac C, Cam = Cam C = Camrose C, Elk = Elk C =
 Elkwater C, Keh = Keh C = Kehiwin C, Mch = Mch C = Michelsen Marsh C, Prk
 = Prk C = Park Lake C, Stn = Stn C = Saskatoon Island C, Yng = Yng C =
 Young's Point C). * position: The spatial position of the
 experimental subject during the main experiment (format: X.Y.Z, where X =
 tray number, Y = row number, Z = column number). * light.treatment: Light
 treatment (format: L01 = full light [*L*~1~], L04 = quarter light
 [*L*~1/4~]). * date.birth: Date of birth of the experimental subject
 (format: MMM.DD.YYYY). * date.last.repro: Date of last reproduction of the
 experimental subject (format: MMM.DD.YYYY). * Status: Status of the
 experimental subject at the end of the experiment (format: DEAD =
 experimental subject tracked until death, NOT_FOCAL = clonal lineage died
 out before producing an experimental subject, NO_REPRO = the experimental
 subject never reproduced, TRUNCATED = experimental subject's data
 collection was truncated before death (e.g., due to contamination)). *
 Exclude: Whether or not the experimental subject was excluded from
 analysis (format: Yes = experimental subject excluded, No = experimental
 subject not excluded). * Sep.23.2024 to Nov.29.2024 [68 columns]: Number
 of offspring produced by the experimental subject on the date given by the
 column header (date/header format: MMM.DD.YYYY; data values format:
 [number] = number of offspring produced, NA = no data (typically because
 (1) the date was before the experimental subject was born or after it
 died, or (2) the experimental subject was excluded from data analysis and
 did not have data associated with it on a particular date). ### #### File:
 CR_strains_temperature_2025_03_08.csv **Description:** Data file
 associated with the measurement of temperature (see Appendix B in our
 article). It has 20 rows (not including the header row) and five columns.
 ##### Variables * ID: ID number of the experimental subject whose neutral
 density filter was tested. * filter.position: The spatial position of the
 experimental subject during the main experiment (format: XYZ, where X =
 tray number, Y = row number, Z = column number). * treatment: Light
 treatment (format: 1 = full light [*L*~1~], 4 = quarter light [*L*~1/4~]).
 * temp.position: The spatial position of the experimental subject during
 temperature data collection (format: XYZ, where X = tray number, Y = row
 number, Z = column number). * temperature: Temperature in degrees Celsius.
 ## Code/software "CR_strains_analysis_2025_11_21.R" is R code
 that analyzes the data and creates figures (except Fig. 1) based on the
 data files. It is available on Zenodo.