DOI: 10.5061/dryad.qp544
title: Data from: Environmental dependency of amphibian-ranavirus genotypic interactions: evolutionary perspectives on infectious diseases

description: The context-dependent investigations of host–pathogen genotypic
 interactions, where environmental factors are explicitly incorporated,
 allow the assessment of both coevolutionary history and contemporary
 ecological influences. Such a functional explanatory framework is
 particularly valuable for describing mortality trends and identifying
 drivers of disease risk more accurately. Using two common North American
 frog species (Lithobates pipiens and Lithobates sylvaticus) and three
 strains of frog virus 3 (FV3) at different temperatures, we conducted a
 laboratory experiment to investigate the influence of host
 species/genotype, ranavirus strains, temperature, and their interactions,
 in determining mortality and infection patterns. Our results revealed
 variability in host susceptibility and strain infectivity along with
 significant host–strain interactions, indicating that the outcome of an
 infection is dependent on the specific combination of host and virus
 genotypes. Moreover, we observed a strong influence of temperature on
 infection and mortality probabilities, revealing the potential for
 genotype–genotype–environment interactions to be responsible for
 unexpected mortality in this system. Our study thus suggests that
 amphibian hosts and ranavirus strains genetic characteristics should be
 considered in order to understand infection outcomes and that the
 investigation of coevolutionary mechanisms within a context-dependent
 framework provides a tool for the comprehensive understanding of disease
 dynamics.
description: InfectionThis file describe the results of infection prevalence per treatment combination. 1. Temperature: COLD (14ºC) and WARM (22ºC). 2. Species: LF (Northern leopard frog), WF (wood frog). 3. Genotypes: we used two clutches of wood frog and one clutch of leopard frog representing 3 genotypes. 4.Strains: we used three ranavirus strain azaC, SsMeV and the wild type wt-FV3, see ur description in the main text. 5. Infection has been assessed by two PCR using published ranavirus MCP primers. The number of infected individual per treatment combination is given. 6. Non-infected: number of non-infected individuals per treatment combination.SurvivalThis data file was tailored for the cox regression analysis of survival/mortality over time per treatment combination. The first eight columns represent independent variables: 1. label is the unique number of an individual. 2. Replicate is the number (1, 2 or 3) of the replicate that has been assigned to an individual. 3. Tank is the tank number to which a given individual was assigned. 4. Temperature is the temperature treatment to which a given individual has been randomly assigned (COLD 14ºC or WARM 22ºC). 5. Species is the taxonomic status of a given individual either LF (Northern leopard frog) or WF (wood frog). 6. Genotypes: we used one clutch of leopard frog (genotype #1) and two clutches of wood frog (genotypes #2 and #3). 7. Strains: we used three ranavirus strain azaC, SsMeV and the wild type wt-FV3, see our description in the main text. 8. Exposure: either an individual is "exposed" or "control". 9. Day_death represents the number of days an individual survived. 10. Censoring is a variable that specify our knowledge or absence of knowledge about an individual death. The value "1" denotes occurrence and observation of an individual's death during the experiment, and the value "0" denotes that an individual was censored because it survived throughout and may have survived after the end of the experiment but we don't really know.Mortality.xlsx