DOI: 10.22002/cas27-h6255
title: Rhodopseudomonas palustrus

description: Fe-metabolizing bacterium that makes parallel membrane sheets for photosynthesis.  Membrane topology is the primary interest.Keywords: photosynthetic membrane sheets, parallel membranes
description: <strong>Tilt Series Date:</strong> 2007-06-22</p>
<strong>Data Taken By:</strong> Bill Tivol</p>
<strong>Species / Specimen:</strong> Rhodopseudomonas palustris</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 1 deg°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Diane Newman, PI, Lars Dietrich, Postdoc, Lars gave me the specimens</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in FEM medium +10% acetate at 30 deg C in bright light.</p>
<strong>Sample Preparation:</strong> quantifoil, 10 nm gold, 100% humidity</p>
  btf2007-06-22-1_slicer4143.jpg: Granule btf2007-06-22-1_slicer4145.jpg: parallel membrane sheet btf2007-06-22-1_slicer4149.jpg: Inner vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-06-22-1/3dimage_367/</p>Rp22Jun07_01.st,  Stack made from raw tilt series by newstack, 973.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-06-22-1/3dimage_367/Rp22Jun07_01.st"
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        Rp22Jun07_01_full.rec,  Reconstruction (Pixel Size 1.25 nm), 4.2 GB  
        
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        Rp22Jun07_01.mrc,  Tilt Series (Pixel Size 0.625 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-06-22-1/rawdata/Rp22Jun07_01.mrc"
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        Rp22Jun07_01.rec,  Reconstruction (Pixel Size 1.25 nm), 131.4 MB  
        
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        Rp22Jun07_01fil2.rec,  Reconstruction (Pixel Size 1.25 nm), 131.4 MB  
        
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        Rp22Jun07_01fil3.rec,  Reconstruction (Pixel Size 1.25 nm) 3x median filtered, 131.4 MB  
        
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