cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5772648
title: Mycelial growth, pathogenicity, aggressiveness and apothecial development of Sclerotinia sclerotiorum isolates from Brazil and the United States in contrasting temperature regimes

description: ABSTRACT Fungi can adapt to environmental conditions and produce different physiological responses. The aim of this study was to verify the existence of Sclerotinia sclerotiorum temperature ecotypes in isolates from Brazil and the USA. Ten S. sclerotiorum isolates from tropical and subtropical regions of Brazil and six isolates from the USA were used to measure mycelial growth, pathogenicity and aggressiveness on bean, canola and soybean, as well as apothecial formation at contrasting temperatures. For mycelial growth, regardless of the origin, all isolates grew faster at 20°C, compared to 27°C. For pathogenicity and aggressiveness, disease severity was greater at 20°C than at 30°C considering all isolates. As regards apothecial production, only Brazilian isolates were capable of producing apothecia with no preconditioning. After preconditioning at 4°C during 40 days, isolates from Brazil and the USA produced apothecia. None of the 16 isolates was capable of producing apothecia at 30oC after 40 days. Results indicated no adaptation of S. sclerotiorum isolates from Brazil to grow or colonize leaflets at higher temperatures, compared to isolates from the USA. Only sclerotia from S. sclerotiorum isolates from Brazil were capable of germinating carpogenically without preconditioning.
DOI: 10.6084/m9.figshare.14279162.v1
title: Action of the essential oil of mint (Mentha arvensis) on the pathogen Lasiodiplodia theobromae in bunches of grapevine cv. Itália

description: ABSTRACT The presence of Lasiodiplodia theobromae (dry rot) on the vine, in irrigated areas of the Brazilian semi-arid region, has been very aggressive, infecting several plant parts. Essential oils show efficient disease control, both for their fungitoxic action, which inhibits mycelial growth and spore germination, and for the presence of elicitor compounds. Thus, this study aimed to obtain L. theobromae control by using the oil from mint (Mentha arvensis). The mycelial growth of L. theobromae was analyzed on Petri plates containing potato-dextrose-agar medium supplemented with the essential oil at the following concentrations: 0; 0.25; 0.5; 1.0; 1.5; 2.0% of mint oil. To evaluate the direct effect of the essential oil on the disease development, the grape bunches were inoculated with the pathogen and, after 4 h, sprayed with solutions containing the same previously described concentrations; then, to evaluate the potential of essential oil in the preventive treatment, the same treatments were applied on grape bunches at 4 hours before inoculation. The mint oil, at all tested concentrations, was capable of inhibiting the mycelial growth of L. theobromae by 100%. In the curative treatment, considering incidence, 0.25% mint was statistically different from the remaining treatments, showing 88% infected berries, while dry rot severity of grape bunches could not be reduced by any of the concentrations. For the preventive treatment, considering incidence, 0.5% mint oil statistically differed from control, showing 86% and 94% infected berries, respectively, and there was greater reduction in dry rot severity, since the disease index was 73.7%, compared to control, which had disease index of 98%.
DOI: 10.6084/m9.figshare.14279165.v1
title: Morphogenetic characterization and resistance reaction of mandarin orange genotypes to Alternaria alternata f. sp. citri

description: ABSTRACT This study aimed to obtain morphogenetic characterization of ten isolates of Alternaria alternata f. sp. citri collected in the state of Paraíba and to evaluate the reaction of five mandarin orange genotypes inoculated with A. alternata f. sp. citri. The experiment was carried out at Federal University of Paraíba. Ten isolates of A. alternata were evaluated for conidial production, mycelial growth rate and final mycelial growth. A. alternata inoculations were done in detached leaves and seedlings of five mandarin orange genotypes, ‘Tangelo Robinson’, ‘Clementina’ Tangerine, ‘Nova’ Tangerine, ‘Lee’ Tangerine and ‘Swatow’ Tangerine, as well as in fruits of tangerine tree cv. ‘Dancy’. In the inoculation of seedlings, isolate 7 was used, which was from Lagoa Seca-PB and presented the best morphological characteristics. Evaluations were performed at 48h after the pathogen inoculation and used a scale of grades. Isolates 2 and 7 presented higher mycelial growth rate and isolates 6 and 7 caused greater disease severity in fruits of cv. ‘Dancy’. Seven divergent groups of A. alternaria f. sp. citri were obtained by the ISSR marker. Genotypes ‘Tangelo Robinson’, ‘Swatow’ Tangerine and ‘Nova’ Tangerine presented greater resistance to Alternaria brown spot under the conditions of the present study.
DOI: 10.6084/m9.figshare.5772441
title: Effectiveness of different fungicide formulations and number of applications in controlling wheat leaf rust

description: ABSTRACT Leaf rust (Puccinia triticina) is one of the most common diseases in wheat crops. For effective control of such disease, leaf application of mixtures of triazole and strobilurin fungicides is recommended. The aim of this study was to evaluate the efficacy of different fungicide formulations and numbers of applications in controlling wheat leaf rust in the cultivar TBIO Pioneiro 2010. Experimental design was completely randomized, 2x2 factorial arrangement, consisting of two fungicide formulations and two numbers of applications, as well as four replicates. Treatments were: azoxystrobin + tebuconazole, three and four applications; trifloxystrobin + prothioconazole, three and four applications; and a control treatment without fungicide application. For treatments with three applications, fungicides were applied at the phenological stages of: elongation, booting and flowering. For treatments with four applications, fungicides were applied at the phenological stages of: tillering, elongation, booting and flowering. The efficiency of fungicides on leaf rust control was analyzed based on the disease severity assessment by calculating the Area Under Disease Progress Curve (AUDPC). Severity data were obtained based on percentage scores of leaf area with visible disease symptoms/signs according to Cobb’s diagrammatic scale. Fungicides with azoxystrobin + tebuconazole and trifloxystrobin + prothioconazole showed efficient control of wheat leaf rust (higher than 85%). For yield components, there was no statistical difference between numbers of applications, but there was a statistical difference between fungicide formulations, except for grain yield. Furthermore, for yield components, all fungicide treatments significantly differed from control.
DOI: 10.6084/m9.figshare.5772441.v1
title: Effectiveness of different fungicide formulations and number of applications in controlling wheat leaf rust

description: ABSTRACT Leaf rust (Puccinia triticina) is one of the most common diseases in wheat crops. For effective control of such disease, leaf application of mixtures of triazole and strobilurin fungicides is recommended. The aim of this study was to evaluate the efficacy of different fungicide formulations and numbers of applications in controlling wheat leaf rust in the cultivar TBIO Pioneiro 2010. Experimental design was completely randomized, 2x2 factorial arrangement, consisting of two fungicide formulations and two numbers of applications, as well as four replicates. Treatments were: azoxystrobin + tebuconazole, three and four applications; trifloxystrobin + prothioconazole, three and four applications; and a control treatment without fungicide application. For treatments with three applications, fungicides were applied at the phenological stages of: elongation, booting and flowering. For treatments with four applications, fungicides were applied at the phenological stages of: tillering, elongation, booting and flowering. The efficiency of fungicides on leaf rust control was analyzed based on the disease severity assessment by calculating the Area Under Disease Progress Curve (AUDPC). Severity data were obtained based on percentage scores of leaf area with visible disease symptoms/signs according to Cobb’s diagrammatic scale. Fungicides with azoxystrobin + tebuconazole and trifloxystrobin + prothioconazole showed efficient control of wheat leaf rust (higher than 85%). For yield components, there was no statistical difference between numbers of applications, but there was a statistical difference between fungicide formulations, except for grain yield. Furthermore, for yield components, all fungicide treatments significantly differed from control.
DOI: 10.6084/m9.figshare.5772648.v1
title: Mycelial growth, pathogenicity, aggressiveness and apothecial development of Sclerotinia sclerotiorum isolates from Brazil and the United States in contrasting temperature regimes

description: ABSTRACT Fungi can adapt to environmental conditions and produce different physiological responses. The aim of this study was to verify the existence of Sclerotinia sclerotiorum temperature ecotypes in isolates from Brazil and the USA. Ten S. sclerotiorum isolates from tropical and subtropical regions of Brazil and six isolates from the USA were used to measure mycelial growth, pathogenicity and aggressiveness on bean, canola and soybean, as well as apothecial formation at contrasting temperatures. For mycelial growth, regardless of the origin, all isolates grew faster at 20°C, compared to 27°C. For pathogenicity and aggressiveness, disease severity was greater at 20°C than at 30°C considering all isolates. As regards apothecial production, only Brazilian isolates were capable of producing apothecia with no preconditioning. After preconditioning at 4°C during 40 days, isolates from Brazil and the USA produced apothecia. None of the 16 isolates was capable of producing apothecia at 30oC after 40 days. Results indicated no adaptation of S. sclerotiorum isolates from Brazil to grow or colonize leaflets at higher temperatures, compared to isolates from the USA. Only sclerotia from S. sclerotiorum isolates from Brazil were capable of germinating carpogenically without preconditioning.
DOI: 10.6084/m9.figshare.14279171
title: Epidemiological aspects of onion downy mildew in the region of Alto Vale do Itajaí, Santa Catarina State, Brazil

description: ABSTRACT With the aim of studying the epidemiology of onion downy mildew caused by Peronospora destructor in the region of Alto Vale do Itajaí, Santa Catarina State, Brazil, an assay was conducted with naturally infected plants in 2014 and 2015 growing seasons. At every seven days, for 19 weeks, the population of sporangia in the air was quantified, the environmental conditions were recorded and the severity in plants was evaluated. Even in an environment favorable to the disease, its onset occurred at nine weeks after transplanting. Epidemics started one week before the first detection of inoculum in the air. The disease progress was represented by the Gompertz model y = 0.42636 *(exp(-442.06555*exp(-0.50749*x))). The yield was 34,930 and 15,773 Kg.ha-1 bulbs in the respective years of evaluation. This epidemiological study can provide information about the beginning of epidemics and can be useful in the validation of a forecast system for onion downy mildew
DOI: 10.6084/m9.figshare.14279171.v1
title: Epidemiological aspects of onion downy mildew in the region of Alto Vale do Itajaí, Santa Catarina State, Brazil

description: ABSTRACT With the aim of studying the epidemiology of onion downy mildew caused by Peronospora destructor in the region of Alto Vale do Itajaí, Santa Catarina State, Brazil, an assay was conducted with naturally infected plants in 2014 and 2015 growing seasons. At every seven days, for 19 weeks, the population of sporangia in the air was quantified, the environmental conditions were recorded and the severity in plants was evaluated. Even in an environment favorable to the disease, its onset occurred at nine weeks after transplanting. Epidemics started one week before the first detection of inoculum in the air. The disease progress was represented by the Gompertz model y = 0.42636 *(exp(-442.06555*exp(-0.50749*x))). The yield was 34,930 and 15,773 Kg.ha-1 bulbs in the respective years of evaluation. This epidemiological study can provide information about the beginning of epidemics and can be useful in the validation of a forecast system for onion downy mildew
DOI: 10.5281/zenodo.4615689
title: Dataset of Laboratory experiments on tsunamigenic discrete subaqueous volcanic eruptions. Part 1: Free surface disturbances

description: This dataset contains all the data used to plot figures 5 and 6 in the manuscript 2020JC016588 "Laboratory experiments on tsunamigenic discrete subaqueous volcanic eruptions. Part 1: Free surface disturbances" by Yaxiong Shen, Colin Whittaker, Emily Lane, James White, William Power, Paraskevi Nomikou submitted to JGR Oceans. The first folder "fountainplumeboundaryfig6" contains data for figure 6. The second folder "jetplumeboundaryfig5" contains data for the fig5. Two Matlab scripts fountainplumeboundary.m and jetplumeboudary.m are used to visualise them respectively.
DOI: 10.6084/m9.figshare.14279173
title: Temporal progress of shot hole in different peach genotypes

description: ABSTRACT Shot hole caused by Wilsonomyces carpophilus is a disease commonly found in peach orchards, affecting the leaf area and consequently reducing fruit production and quality. Considering that the behavior of this disease is scarcely known, this study aimed to evaluate the temporal progress throughout the production cycle in different peach genotypes. The experiment was carried out in a peach orchard at “Instituto Federal Catarinense”-IFC / Rio do Sul Campus. Evaluated genotypes were: Granada, Dourado 1, Dourado 2, Douradão, Ouro, Chimarrita, Flordaprince, Premier, Aurora and Della Nona. Four plants from each genotype were selected and estimated disease severity was weekly evaluated after the beginning of sprouting, on 16 leaves from each plant, using a diagrammatic scale. The disease progress curve for each genotype was adjusted to a logistic model, since the observed severity corresponded to the model and was confirmed by the consistency between the estimated points and the residue. Genotypes Flordaprince and Premier showed the highest disease rates (0.329), while Douradão (0.223) had the lowest rate. Genotype Dourado 1 showed the highest severity peak, 10.5%, and Granada had the lowest disease accumulation, 4.5%. Peach genotypes had different temporal progress behaviors to shot hole.
DOI: 10.6084/m9.figshare.14279173.v1
title: Temporal progress of shot hole in different peach genotypes

description: ABSTRACT Shot hole caused by Wilsonomyces carpophilus is a disease commonly found in peach orchards, affecting the leaf area and consequently reducing fruit production and quality. Considering that the behavior of this disease is scarcely known, this study aimed to evaluate the temporal progress throughout the production cycle in different peach genotypes. The experiment was carried out in a peach orchard at “Instituto Federal Catarinense”-IFC / Rio do Sul Campus. Evaluated genotypes were: Granada, Dourado 1, Dourado 2, Douradão, Ouro, Chimarrita, Flordaprince, Premier, Aurora and Della Nona. Four plants from each genotype were selected and estimated disease severity was weekly evaluated after the beginning of sprouting, on 16 leaves from each plant, using a diagrammatic scale. The disease progress curve for each genotype was adjusted to a logistic model, since the observed severity corresponded to the model and was confirmed by the consistency between the estimated points and the residue. Genotypes Flordaprince and Premier showed the highest disease rates (0.329), while Douradão (0.223) had the lowest rate. Genotype Dourado 1 showed the highest severity peak, 10.5%, and Granada had the lowest disease accumulation, 4.5%. Peach genotypes had different temporal progress behaviors to shot hole.
DOI: 10.6084/m9.figshare.5772294
title: Influence of Macrophomina phaseolina inoculation methodologies on the performance of soybean cultivars

description: ABSTRACT Charcoal rot caused by the fungus Macrophomina phaseolina is one of the major diseases that causes rot in the root system and stem of soybean plants, and its occurrence has increased at every harvest season. The aim of this study was to evaluate the influence of M. phaseolina inoculation methodologies on the performance of soybean cultivars. The experiment was conducted in a completely randomized design, in sub-subdivided plots, containing two soybean cultivars (NA 7337 RR and CD 2737 RR), six inoculation methods (non-inoculated seed, seed inoculated for 48 hours, seed inoculated for 72 hours, soil infestation with three rice grains, soil infestation with six rice grains and soil infestation with nine rice grains) and three evaluation periods (20, 40 and 60 days after sowing) with 6 replicates. Evaluations were performed by measuring the height of plants, diameter of the colon, number of leaves, length and width of leaves and incidence of the fungus Macrophomina phaseolina. Results of the experiments indicated that the inoculation method using rice grains inoculated with the fungus M. phaseolina provided greater damage to the cultivar NA 7337 RR, but the cultivar CD 2737 RR was influenced by the method of inoculating directly in the soybean seed. The cultivar NA 7337 RR showed to be more tolerant to the fungus Macrophomina phaseolina. In the evaluation periods, the indirect quantification parameters of the disease progressively evolved at 20, 40 and 60 days. Only control treatment did not present the fungus M. phaseolina and all employed inoculation methods provided the development of the fungus in soybean plants. All inoculation methods were efficient in inoculating M. phaseolina in soybean plants. The method of directly inoculating in the seed for 48 and 72 hours developed early symptoms
DOI: 10.6084/m9.figshare.6045014
title: Identification of protective isolates of Citrus tristeza virus (CTV) for Citrus sinensis (L.) Osbeck

description: ABSTRACT Citrus tristeza virus (CTV) causes significant yield losses to sweet orange [Citrus sinensis (L.) Osbeck] and its control has been carried out especially with premunization. The aim of this study was to analyze the variability of severe and mild CTV isolates from citrus plants inoculated and kept in a greenhouse and field samples collected from a commercial orchard located in the city of Rolândia, Paraná State (PR). To determine the variability and the genetic diversity of isolates, symptoms were evaluated and the techniques RT-PCR and RFLP were employed, using the specific oligonucleotides HCP1 / HCP2 and subsequent sequencing of the amplified fragments. In the evaluation of stem pitting, the isolates kept in greenhouse induced mild symptoms, except for the severe isolate Capão Bonito. The most severe symptoms occurred in samples located in the field. The multivariate analyses showed that CTV isolates tend to cluster according to the severity of symptoms and the environmental conditions to which they were exposed, forming distinct groupings among samples from the field and the greenhouse. The dendrogram generated from the sequencing of isolates and the multivariate analyses revealed that the isolate from the sample “Forte Arapongas” presented greater similarity with the severe pattern control from Capão Bonito. The isolates identified as mild and from the samples Pêra IAC and Rolândia 5 presented greater similarity. Host plants maintained in the field can be considered to have more variability of isolates.
DOI: 10.6084/m9.figshare.6045014.v1
title: Identification of protective isolates of Citrus tristeza virus (CTV) for Citrus sinensis (L.) Osbeck

description: ABSTRACT Citrus tristeza virus (CTV) causes significant yield losses to sweet orange [Citrus sinensis (L.) Osbeck] and its control has been carried out especially with premunization. The aim of this study was to analyze the variability of severe and mild CTV isolates from citrus plants inoculated and kept in a greenhouse and field samples collected from a commercial orchard located in the city of Rolândia, Paraná State (PR). To determine the variability and the genetic diversity of isolates, symptoms were evaluated and the techniques RT-PCR and RFLP were employed, using the specific oligonucleotides HCP1 / HCP2 and subsequent sequencing of the amplified fragments. In the evaluation of stem pitting, the isolates kept in greenhouse induced mild symptoms, except for the severe isolate Capão Bonito. The most severe symptoms occurred in samples located in the field. The multivariate analyses showed that CTV isolates tend to cluster according to the severity of symptoms and the environmental conditions to which they were exposed, forming distinct groupings among samples from the field and the greenhouse. The dendrogram generated from the sequencing of isolates and the multivariate analyses revealed that the isolate from the sample “Forte Arapongas” presented greater similarity with the severe pattern control from Capão Bonito. The isolates identified as mild and from the samples Pêra IAC and Rolândia 5 presented greater similarity. Host plants maintained in the field can be considered to have more variability of isolates.
DOI: 10.6084/m9.figshare.7185947
title: Inoculation of Curvularia eragrostidis in yam plants (Dioscorea alata) cv. São Tomé

description: ABSTRACT The aim of this study was to select the method of C. eragrostidis inoculation in yam seedlings (Dioscorea alata) cv. São Tomé to determine the infection and the latency period. The isolate of C. eragrostidis was obtained from yam leaves showing symptoms of the disease. Plants aged 45 days old, cultivated in a greenhouse, were inoculated with the pathogen, as follows: T1 – Spore suspension (1x10 conidia mL-1); T2 – Colonial disks of 10mm fixed with adhesive tape (two on the abaxial surface and one on the adaxial surface); T3 - Brushing of spores; T4 - Cotton soaked in the inoculum suspension; T5 - Control (plants sprayed with sterile distilled water). After 72h of inoculation, the disease incidence was daily evaluated and the severity was assessed on the 15th day. The obtained results revealed that the spore suspension spraying method was more efficient for C. eragrostidis inoculation in yam seedlings, causing the first symptoms to appear from the third day after inoculation.
DOI: 10.5281/zenodo.4615688
title: Dataset of Laboratory experiments on tsunamigenic discrete subaqueous volcanic eruptions. Part 1: Free surface disturbances

description: This dataset contains all the data used to plot figures 5 and 6 in the manuscript 2020JC016588 "Laboratory experiments on tsunamigenic discrete subaqueous volcanic eruptions. Part 1: Free surface disturbances" by Yaxiong Shen, Colin Whittaker, Emily Lane, James White, William Power, Paraskevi Nomikou submitted to JGR Oceans. The first folder "fountainplumeboundaryfig6" contains data for figure 6. The second folder "jetplumeboundaryfig5" contains data for the fig5. Two Matlab scripts fountainplumeboundary.m and jetplumeboudary.m are used to visualise them respectively.