cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.10303504
title: Supporting information

description: Supporting information
S1 Fig. Co-immunoprecipitation between Nav1.5 and 14-3-3 isoforms: 48 hours after transient overexpression of 14-3-3 β/α (encoded by YWHAB), 14-3-3 γ (encoded by YWHAG), 14-3-3 ε (encoded by YWHAE), 14-3-3 σ (encoded by SFN) in tsA201 expressing Nav1.5 or Nav1.5-FLAG. Endogenous α-1-syntrophin was used as a positive control for co-immunoprecipitation with Nav1.5, and α-actin as a negative control.
S2 Fig. Co-immunoprecipitation between Nav1.5 and 14-3-3 isoforms: 48 hours after transient overexpression of 14-3-3η (encoded by YWHAH), 14-3-3θ (encoded by YWHAQ) and 14-3-3ζ/δ (encoded by YWHAZ) in tsA201 expressing Nav1.5 or Nav1.5-FLAG. Endogenous α-1-syntrophin was used as a positive control for co-immunoprecipitation with Nav1.5, and α-actin as a negative control. Intensity of Nav1.5-immunoprecipitated 14-3-3η-HA was normalized to the intensity of the total 14-3-3η-HA divided by the intensity of α-actin. Data are presented as mean ± SEM from three biological replicates and are normalized to the control condition ("+ Nav1.5"). Individual p-value, calculated with one-sample two-tailed t-test with hypothetical mean value=1, is indicated in the panel.
S3 Fig. Endogenous expression of 14-3-3 isoforms in tsA201 cell line. Conventional RT-PCR on tsA201 WT cells showing endogenous transcripts of all seven 14-3-3 isoforms (14-3-3β/α encoded by YWHAB, 14-3-3γ encoded by YWHAG, 14-3-3ε encoded by YWHAE, 14-3-3σ encoded by SFN, 14-3-3η encoded by YWHAH, 14-3-3θ encoded by YWHAQ, and 14-3-3ζ/δ encoded by YWHAZ) as well as housekeeping genes (glyceraldehyde-3-phosphate dehydrogenase encoded by GAPDH, TATA-binding protein encoded by TBP, and proteasome 20S subunit β4 encoded by PSMB4) with their expected amplicon sizes.
S4 Fig. Validation of difopein as an efficient competitor for 14-3-3/ligand interaction. Co-immunoprecipitation analysis was performed 48 hours after transient overexpression of 14-3-3 in tsA201 WT cells. (a) Difopein strongly co-immunoprecipitated with 14-3-3 proteins, while the mutant of difopein did not. YFP-tagged difopein and its mutant were revealed with an anti-GFP antibody. (b) Difopein stabilized 14-3-3 dimers occupying its binding grooves, preventing 14-3-3 interactions with other ligands. The mutant of difopein did not stabilize 14-3-3 dimers and hence could be used as the closest relevant control for experiments involving difopein.  
S5 Fig. Investigation of endogenous 14-3-3η protein level in tsA201. Immunoblotting analysis was performed 48 hours after transient overexpression of an empty vector or 14-3-3-HA proteins in tsA201 WT cells. Anti-14-3-3η antibody specifically detected overexpressed 14-3-3η and to a much lower extent 14-3-3ε. However, no noticeable level of endogenous 14-3-3η was detected in tsA201 transfected with an empty vector. The successful overexpression of all seven mammalian 14-3-3 isoforms was revealed with an anti-HA antibody. Ponceau S staining was performed to verify equal protein loading.
DOI: 10.5281/zenodo.10303505
title: Supporting information

description: Supporting information
S1 Fig. Co-immunoprecipitation between Nav1.5 and 14-3-3 isoforms: 48 hours after transient overexpression of 14-3-3 β/α (encoded by YWHAB), 14-3-3 γ (encoded by YWHAG), 14-3-3 ε (encoded by YWHAE), 14-3-3 σ (encoded by SFN) in tsA201 expressing Nav1.5 or Nav1.5-FLAG. Endogenous α-1-syntrophin was used as a positive control for co-immunoprecipitation with Nav1.5, and α-actin as a negative control.
S2 Fig. Co-immunoprecipitation between Nav1.5 and 14-3-3 isoforms: 48 hours after transient overexpression of 14-3-3η (encoded by YWHAH), 14-3-3θ (encoded by YWHAQ) and 14-3-3ζ/δ (encoded by YWHAZ) in tsA201 expressing Nav1.5 or Nav1.5-FLAG. Endogenous α-1-syntrophin was used as a positive control for co-immunoprecipitation with Nav1.5, and α-actin as a negative control. Intensity of Nav1.5-immunoprecipitated 14-3-3η-HA was normalized to the intensity of the total 14-3-3η-HA divided by the intensity of α-actin. Data are presented as mean ± SEM from three biological replicates and are normalized to the control condition ("+ Nav1.5"). Individual p-value, calculated with one-sample two-tailed t-test with hypothetical mean value=1, is indicated in the panel.
S3 Fig. Endogenous expression of 14-3-3 isoforms in tsA201 cell line. Conventional RT-PCR on tsA201 WT cells showing endogenous transcripts of all seven 14-3-3 isoforms (14-3-3β/α encoded by YWHAB, 14-3-3γ encoded by YWHAG, 14-3-3ε encoded by YWHAE, 14-3-3σ encoded by SFN, 14-3-3η encoded by YWHAH, 14-3-3θ encoded by YWHAQ, and 14-3-3ζ/δ encoded by YWHAZ) as well as housekeeping genes (glyceraldehyde-3-phosphate dehydrogenase encoded by GAPDH, TATA-binding protein encoded by TBP, and proteasome 20S subunit β4 encoded by PSMB4) with their expected amplicon sizes.
S4 Fig. Validation of difopein as an efficient competitor for 14-3-3/ligand interaction. Co-immunoprecipitation analysis was performed 48 hours after transient overexpression of 14-3-3 in tsA201 WT cells. (a) Difopein strongly co-immunoprecipitated with 14-3-3 proteins, while the mutant of difopein did not. YFP-tagged difopein and its mutant were revealed with an anti-GFP antibody. (b) Difopein stabilized 14-3-3 dimers occupying its binding grooves, preventing 14-3-3 interactions with other ligands. The mutant of difopein did not stabilize 14-3-3 dimers and hence could be used as the closest relevant control for experiments involving difopein.  
S5 Fig. Investigation of endogenous 14-3-3η protein level in tsA201. Immunoblotting analysis was performed 48 hours after transient overexpression of an empty vector or 14-3-3-HA proteins in tsA201 WT cells. Anti-14-3-3η antibody specifically detected overexpressed 14-3-3η and to a much lower extent 14-3-3ε. However, no noticeable level of endogenous 14-3-3η was detected in tsA201 transfected with an empty vector. The successful overexpression of all seven mammalian 14-3-3 isoforms was revealed with an anti-HA antibody. Ponceau S staining was performed to verify equal protein loading.
DOI: 10.6084/m9.figshare.23126816
title: Datasets for <em>Pachypodium lamerei</em>, <em>Apocynum venetum</em>, <em>Asclepias syriaca</em> and <em>Calotropis procera</em> genomes

description: Dataset for <i>Pachypodium lamerei</i> genome- Genome (Pachypodium_lamerei_genome*.fasta)- Predicted transcripts (Pachypodium_lamerei_v1.0.transcripts)- Predicted coding sequences (Pachypodium_lamerei_v1.0.cds)- Predicted proteins (Pachypodium_lamerei_v1.0.pep)- Genome annotation file (Pachypodium_lamerei_v1.0.gtf)- Gene functional annotation (Pachypodium_lamerei_Trinotate.xls)Dataset for <i>Apocynum venetum</i> genome- Genome (Apocynum_venetum_GCA_019593545.1.fna)- Predicted transcripts (Apocynum_venetum.transcripts)- Predicted coding sequences (Apocynum_venetum.cds)- Predicted proteins (Apocynum_venetum.pep)- Genome annotation file (Apocynum_venetum_v1.0_UT.gtf)- Gene functional annotation (Apocynum_venetum_Trinotate.xls)Dataset for <i>Asclepias syriaca</i> genome- Genome (Asclepias_syriaca_GCA_027405835.1.fna)- Predicted transcripts (Asclepias_syriaca.transcripts)- Predicted coding sequences (Asclepias_syriaca.cds)- Predicted proteins (Asclepias_syriaca.pep)- Genome annotation file (Asclepias_syriaca_UT.gtf)- Gene functional annotation (Asclepias_syriaca_Trinotate.xls)Dataset for <i>Calotropis procera</i> genome- Genome (Calotropis_procera_GCA_004801955.1.fna)- Predicted transcripts (Calotropis_procera.transcripts)- Predicted coding sequences (Calotropis_procera.cds)- Predicted proteins (Calotropis_procera.pep)- Genome annotation file (Calotropis_procera_UT.gtf)- Gene functional annotation (Calotropis_procera_Trinotate.xls)Database containing functionally characterized proteins involved in shikimate, methyl erythritol phosphate (MEP), iridoid and MIA pathways (MIA_db.pep)
DOI: 10.6084/m9.figshare.25266367
title: Klebsiella pneumoniae outbreak scenario

description: Fastq reads used for a simulated Klebsiella pneumoniae outbreak scenario in a hospital NICU.
DOI: 10.15139/s3/yqahqu
title: Dataset for Celeste Carberry's Doctoral Dissertation

description: The following dataset contains supplementary tables for Celeste Carberry's Doctoral Dissertation (2024).
DOI: 10.25625/ejvbhd/avjf2m
title: 2-Cl_Ar_0_275_K_300_K_1p0_mm_18x4_min_0p4_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/f88wrj
title: 1-Cl_Ar_0_275_K_300_K_1p0_mm_24x4_min_0p4_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/3tuseq
title: 0-Cl1_Ar_0_335_K_365_K_1p0_mm_15x4_min_0p4_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/emh9jt
title: 0-Br1_Ar_0_345_K_365_K_1p0_mm_10x4_min_0p35_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/uhqxjx
title: c6-6Ph_Ar_0_395_425_K_300_K_1p0_mm_18x4_min_0p4_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/vdye7x
title: 2-Br_Ar_0_285_K_300_K_1p0_mm_8x4_min_0p6_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/wgag14
title: 1-Br_Ar_0_285_K_300_K_1p0_mm_10x4_min_0p6_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/egykq7
title: ttBuMeOH_Ar_0_355_K_425_K_1p0_mm_4x4_min_0p35_bar.dat

description: :unav
DOI: 10.25625/ejvbhd/hw3tak
title: 1-Br_Ar_0_285_K_300_K_1p0_mm_10x4_min_0p6_bar.dat

description: :unav
DOI: 10.6084/m9.figshare.25266406
title: teaseq_unfilt.h5mu

description: Output of the panpipes multimodal ingestion tutorial for rna, prot and atac.
DOI: 10.6084/m9.figshare.25266406.v1
title: teaseq_unfilt.h5mu

description: Output of the panpipes multimodal ingestion tutorial for rna, prot and atac.
DOI: 10.5883/ds-ibilp08
title: The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths

description: DNA barcode data hosted in the Data Portal of the Barcode of Life Data Systems. Records consist of specimen metadata, specimen images, and sequence data.
DOI: 10.25625/ctg8td/sbsvfw
title: Microwave_Br_Overview.tab

description: :unav
DOI: 10.25625/ctg8td/qrndgi
title: 2_Br_g_primeG.xyz

description: :unav
DOI: 10.25625/ctg8td/oltw16
title: B0_Overview.tab

description: :unav
DOI: 10.25625/ctg8td/isfh3m
title: 1_Br_g_primeG.xyz

description: :unav
DOI: 10.48579/pro/rgckqq
title: Fish biodiversity inventories of the port of Calvi, L'Île-Rousse, Saint-Florent and STARESO, Corsica (France) using citizen science eDNA techniques in 2022

description: This dataset contains fish occurrences in Mediterranean ports from an eDNA citizen science study using specially-designed citizen science eDNA kits as part of the EMFF-funded project QUAMPO (Grant agreement number: PFEA800219DM0940001) on port water quality. In total, 452 occurrences were recorded, in 4 ports of Haute-Corse (France): port of Calvi, port of L'Île-Rousse, port of Saint-Florent and at the marine station STARESO in Calvi. Citizen (pupils in 5th grade at 2 local schools) took part in the June sampling in the port of L'Île-Rousse. Among these occurrences, 422 were identified at the species-level and 30 at the genus-level. These fish inventory represented 63 species and 9 genuses. Originally, one sampling occasion for each port was foreseen in June 2022. However, we added a second sampling occasion in October 2022 to describe fish communities in ports after the storm that hit Corsica on August, 18 2022. This project is the first project using eDNA and citizen science in ports to study fish biodiversity.
DOI: 10.48579/pro/rgckqq/bdwc7d
title: occurrence_fish_Corsica2022_QUAMPO_V4.tab

description: :unav
DOI: 10.48579/pro/rgckqq/igqo2z
title: occurrence_fish_Corsica2022_QUAMPO_V5.txt

description: :unav
DOI: 10.48579/pro/rgckqq/utzrks
title: occurrence_fish_Corsica2022_QUAMPO.tab

description: This sheet is used to record a list of fish species in 4 ports of Corsica, France: port of Calvi, port of the marine station of Calvi (STARESO), port of L'Île-Rousse and port of Saint-Florent, in June, October and November 2022.
DOI: 10.25625/ctg8td/p4lci8
title: 1_Cl_g_primeG.xyz

description: :unav
DOI: 10.25625/ctg8td/cxv5zp
title: 2_Br_gG_prime.xyz

description: :unav
DOI: 10.25625/ctg8td/u0wczk
title: 2_Cl_gG_prime.xyz

description: :unav
DOI: 10.25625/ctg8td/zkry7o
title: 1_Br_gG_prime.xyz

description: :unav
DOI: 10.25625/ctg8td/aqx38a
title: 1_Cl_gG_prime.xyz

description: :unav
DOI: 10.5281/zenodo.10604936
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (BIORXIV/2024/574823)
DOI: 10.5281/zenodo.10604935
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data as well as the code for analysis used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (https://doi.org/10.1101/2024.02.01.574823)
DOI: 10.5281/zenodo.10624783
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data as well as the code for analysis used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (https://doi.org/10.1101/2024.02.01.574823)
DOI: 10.5281/zenodo.10663546
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data as well as the code for analysis used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (https://doi.org/10.1101/2024.02.01.574823)
DOI: 10.5281/zenodo.10666629
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data as well as the code for analysis used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (https://doi.org/10.1101/2024.02.01.574823)
DOI: 10.5281/zenodo.10671707
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data as well as the code for analysis used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (https://doi.org/10.1101/2024.02.01.574823)
DOI: 10.5281/zenodo.10693755
title: Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

description: Macroivertebrate community and environmental data as well as the code for analysis used in the manuscript Beck et al. (2024) ''Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study'' (https://doi.org/10.1101/2024.02.01.574823)
DOI: 10.25625/ctg8td/hitn44
title: 2_Cl_g_primeG.xyz

description: :unav
DOI: 10.25625/ctg8td/6mzl8q
title: 1_79Br_gG_prime_form.out

description: :unav
DOI: 10.25625/ctg8td/pu0rcm
title: 2_81Br_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/uqjep2
title: 1_81Br_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/fzzlgp
title: 1_79Br_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/faeruk
title: 2_79Br_gG_prime_form.out

description: :unav
DOI: 10.25625/ctg8td/p67zwk
title: 1_81Br_gG_prime_form.out

description: :unav
DOI: 10.25625/ctg8td/kuxban
title: 2_79Br_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/txemwz
title: 2_81Br_gG_prime_form.out

description: :unav
DOI: 10.25625/ctg8td/ptysrv
title: 1_35Cl_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/et3pyj
title: 2_35Cl_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/4ujtl5
title: 1_35Cl_gG_prime_form.out

description: :unav
DOI: 10.6084/m9.figshare.12452048
title: Additional file 10 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 10:. Supplementary table 10. Multivariate linear regression of 7888 CpG sites using group and HBV marker
DOI: 10.25625/ctg8td/ogekzc
title: 2_37Cl_gG_prime_form.out

description: :unav
DOI: 10.6084/m9.figshare.12452048.v1
title: Additional file 10 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 10:. Supplementary table 10. Multivariate linear regression of 7888 CpG sites using group and HBV marker
DOI: 10.25625/ctg8td/7o0iby
title: 1_37Cl_g_primeG_form.out

description: :unav
DOI: 10.25625/ctg8td/2yf06z
title: 2_35Cl_gG_prime_form.out

description: :unav
DOI: 10.25625/ctg8td/uski9m
title: 1_37Cl_gG_prime_form.out

description: :unav
DOI: 10.25625/ctg8td/x72nrw
title: Microwave_Cl_Overview.tab

description: :unav
DOI: 10.6084/m9.figshare.12452078
title: Additional file 2 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 2:. Supplementary table 2. Multivariate linear regression of 7888 CpG sites
DOI: 10.6084/m9.figshare.12452078.v1
title: Additional file 2 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 2:. Supplementary table 2. Multivariate linear regression of 7888 CpG sites
DOI: 10.25625/s5pegu/um0vwh
title: 13831_875_513avgs.dat

description: :unav
DOI: 10.25625/s5pegu/cefcwf
title: 16947_300_724avgs.dat

description: :unav
DOI: 10.25625/s5pegu/c4ipm7
title: 13510_557_852avgs.dat

description: :unav
DOI: 10.25625/s5pegu/m88fty
title: 11282_610_3040avgs.dat

description: :unav
DOI: 10.6084/m9.figshare.12452087
title: Additional file 3 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 3:. Supplementary table 3. Multifactorial ANOVA analysis of 7888 CpG sites
DOI: 10.25625/s5pegu/ecybjc
title: 13924_704_265avgs.dat

description: :unav
DOI: 10.25625/s5pegu/5znfai
title: 13476_585_2264avgs.dat

description: :unav
DOI: 10.25625/s5pegu/wswo5n
title: 13284_156_1363avgs.dat

description: :unav
DOI: 10.6084/m9.figshare.12452087.v1
title: Additional file 3 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 3:. Supplementary table 3. Multifactorial ANOVA analysis of 7888 CpG sites
DOI: 10.25625/s5pegu/ud7dqe
title: 13471_569_2889avgs.dat

description: :unav
DOI: 10.25625/s5pegu/hwutfo
title: 10707_742_209avgs.dat

description: :unav
DOI: 10.25625/s5pegu/qgusgh
title: 11808_540_1774avgs.dat

description: :unav
DOI: 10.25625/s5pegu/wookml
title: 14390_406_1449avgs.dat

description: :unav
DOI: 10.6084/m9.figshare.12452114
title: Additional file 7 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 7:. Supplementary table 7. Multifactorial ANOVA analysis of 4325 CpG sites
DOI: 10.6084/m9.figshare.24782532.v1
title: Supplementary Material for: Oxytocin receptor-expressing neurons in the medial preoptic area are essential for lactation, whereas those in the lateral septum are not critical for maternal behavior.

description: Introduction: In nurturing systems, the oxytocin (Oxt)–oxytocin receptor (Oxtr) system is important for parturition, and essential for lactation and parental behavior. Among the nerve nuclei that express Oxtr, the lateral septal nucleus (LS) and medial preoptic area (MPOA) are representative regions that control maternal behavior. Methods: We investigated the role of Oxtr- and Oxtr-expressing neurons, located in the LS and MPOA, in regulating maternal behavior by regulating Oxtr expression in a region-specific manner using recombinant mice and adeno-associated viruses. We quantified the prolactin (Prl) concentrations in the pituitary gland and plasma when Oxtr expression in the MPOA was reduced. Results: The endogenous Oxtr gene in the neurons of the LS did not seem to play an essential role in maternal behavior. Conversely, decreased Oxtr expression in the MPOA increased the frequency of pups being left outside the nest and reduced their survival rate. Deletion of Oxtr in MPOA neurons prevented elevation of Prl levels in plasma and pituitary at postpartum day 2. Discussion/Conclusion: Oxtr-expressing neurons in the MPOA are involved in the postpartum production of Prl. We confirmed the essential functions of Oxtr-expressing neurons and the Oxtr gene itself in the MPOA for the sustainability of maternal behavior, which involved Oxtr-dependent induction of Prl.
DOI: 10.25625/s5pegu/ikvjfk
title: 12827_190_533avgs.dat

description: :unav
DOI: 10.25625/s5pegu/iaocvz
title: 12806_346_719avgs.dat

description: :unav
DOI: 10.6084/m9.figshare.12452114.v1
title: Additional file 7 of The signature of HBV-related liver disease in peripheral blood mononuclear cell DNA methylation

description: Additional file 7:. Supplementary table 7. Multifactorial ANOVA analysis of 4325 CpG sites
DOI: 10.6084/m9.figshare.24782532.v4
title: Supplementary Material for: Oxytocin receptor-expressing neurons in the medial preoptic area are essential for lactation, whereas those in the lateral septum are not critical for maternal behavior.

description: Introduction: In nurturing systems, the oxytocin (Oxt)–oxytocin receptor (Oxtr) system is important for parturition, and essential for lactation and parental behavior. Among the nerve nuclei that express Oxtr, the lateral septal nucleus (LS) and medial preoptic area (MPOA) are representative regions that control maternal behavior. Methods: We investigated the role of Oxtr- and Oxtr-expressing neurons, located in the LS and MPOA, in regulating maternal behavior by regulating Oxtr expression in a region-specific manner using recombinant mice and adeno-associated viruses. We quantified the prolactin (Prl) concentrations in the pituitary gland and plasma when Oxtr expression in the MPOA was reduced. Results: The endogenous Oxtr gene in the neurons of the LS did not seem to play an essential role in maternal behavior. Conversely, decreased Oxtr expression in the MPOA increased the frequency of pups being left outside the nest and reduced their survival rate. Deletion of Oxtr in MPOA neurons prevented elevation of Prl levels in plasma and pituitary at postpartum day 2. Discussion/Conclusion: Oxtr-expressing neurons in the MPOA are involved in the postpartum production of Prl. We confirmed the essential functions of Oxtr-expressing neurons and the Oxtr gene itself in the MPOA for the sustainability of maternal behavior, which involved Oxtr-dependent induction of Prl.
DOI: 10.25625/s5pegu/gfru4d
title: 10763_856_303avgs.dat

description: :unav
DOI: 10.25625/s5pegu/drpid7
title: 12832_668_528avgs.dat

description: :unav