DOI: 10.5061/dryad.3ft30
title: Data from: De novo transcriptome characterization of a sterilizing trematode parasite (Microphallus sp.) from two species of New Zealand snails

description: Snail-borne trematodes represent a large, diverse, and evolutionarily,
 ecologically, and medically important group of parasites, often imposing
 strong selection on their hosts and causing host morbidity and mortality.
 Even so, there are very few genomic and transcriptomic resources available
 for this important animal group. We help to fill this gap by providing
 transcriptome resources from trematode metacercariae infecting two
 congeneric snail species, Potamopyrgus antipodarum and P. estuarinus. This
 genus of New Zealand snails has gained prominence in large part through
 the development of P. antipodarum and its sterilizing trematode parasite
 Microphallus livelyi into a textbook model for host-parasite
 coevolutionary interactions in nature. By contrast, the interactions
 between Microphallus trematodes and P. estuarinus, an estuary-inhabiting
 species closely related to the freshwater P. antipodarum, are relatively
 unstudied. Here, we provide the first annotated transcriptome assemblies
 from Microphallus isolated from P. antipodarum and P. estuarinus. We also
 use these transcriptomes to produce genomic resources that will be broadly
 useful to those interested in host-parasite coevolution, local adaption,
 and molecular evolution and phylogenetics of this and other
 snail-trematode systems. Analyses of the two Microphallus transcriptomes
 revealed that the two trematode types are more genetically differentiated
 from one another than are M. livelyi infecting different populations of P.
 antipodarum, suggesting that the Microphallus infecting P. estuarinus
 represent a distinct lineage. We also provide a promising set of candidate
 genes likely involved in parasitic infection and response to salinity
 stress.
description: Fst_per_SNP_dataFst per SNP between PA-Microphallus and PE-Microphallus 1) with both sets of reads mapped to the PA-Microphallus set of orthologous transcripts, and 2)with both sets of reads mapped to the PE-Microphallus set of orthologous transcripts.Theta_per_transcript_dataWe used Popoolation to estimate Watterson's theta for each transcript in our set of orthologous transcript for 1) PA-Microphallus, and 2) PE-Microphallus.Pairwise_distance_dataWe used Clustal Omega to pairs of orthologous transcripts and calculate Jukes-Cantor-corrected pairwise molecular distance between PA-Microphallus and PE-MicrophallusMicrosatellites_and_primer_dataWe used MISA and Primer3 to identify potential microsatellite loci and predict primer sequences for amplification from our set of orthologous transcripts for both PA-Microphallus and PE-Microphallus. This file contains the locus and primer information (Tables S8 and S9 in manuscript).PE-Microphallus_annotated_transcriptomeFasta formatted annotated transcriptome for PE-Microphallus The assembly was generated using Trinity, followed by TransDecoder, then CD-HIT-EST. The transcriptome was annotated using blastx and Blast2GO.PA-Microphallus_annotated_transcriptomeFasta formatted annotated transcriptome for PA-Microphallus The assembly was generated using Trinity, followed by TransDecoder, then CD-HIT-EST. The transcriptome was annotated using blastx and Blast2GO.