DOI: 10.5061/dryad.f3r67
title: Data from: The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles

description: The pine weevil (Hylobius abietis, Coleoptera: Curculionidae) is an
 important pest of conifer seedlings in Europe. Despite its economic
 importance, little is known about the composition of its gut microbial
 community and the role it plays in mediating the weevil's ability to
 utilize conifers as a food source. Here, we characterized the gut
 bacterial communities of different populations of H. abietis across Europe
 and compared them to those of other beetles that occupy similar ecological
 niches. We demonstrate that the microbial community of H. abietis is
 similar at higher taxonomic levels (family and genus) across locations in
 Europe, with Wolbachia as the dominant microbe, followed by Enterobacteria
 and Firmicutes. Despite this similarity, we observed consistent
 differences between countries and locations, but not sexes. Our
 meta-analysis demonstrates that the gut bacterial community of the pine
 weevil is very similar to that of bark beetles that also exploit conifers
 as a food source. The Enterobacteriaceae symbionts of both host taxa are
 especially closely related phylogenetically. Conversely, the microbiota of
 H. abietis is distinct from that of closely related weevils feeding on
 non-conifer food sources, suggesting that the microbial community of the
 pine weevil is determined by the environment and may be relevant to host
 ecology. Furthermore, several H. abietis-associated members of the
 Enterobacteriaceae family are known to contain genes involved in terpenoid
 degradation. As such, we hypothesize that the gut microbial community is
 important for the utilization of conifer seedlings as a food source,
 either through the detoxification of plant secondary metabolites or
 supplementation of essential nutrients.
description: Quality filtered 454
 sequence data454_seqs.fnaMapping fileMapping file for quality trimmed 454 sequences of bacterial 16s rRNA from the gut of Hylobius abietisMapping_file.txtOTU table generated after closed reference OTU picking strategyOTU_table_closed_reference.txtOTU table generated after open reference OTU picking strategyOTU_table_open_reference.txtPhylogenetic tree of bacterial sequences from different beetles feeding on coniferous and non coniferous food sourcesThe phylogeny was reconstructed with FastTree using the GTR model.Phylogenetic_tree_meta-analysis.nwkAlignment_for_meta-analysisOur data set on H. abietis-associated bacteria was expanded by the addition of 308 bacterial sequences from GenBank associated with ten different insect host species belonging to four genera. These sequences included bacterial taxa found in the gut microbiota of five species of bark beetles belonging to two genera that feed on conifers, as well as five weevil species belonging to two genera that feed on red palm trees and ornamental plants, respectively . We only selected culture-independent studies. Database sequences were aligned with those obtained in this study using SINA. The alignment was imported into ARB together with the SILVA 115 database and a phylogenetic tree was constructed. We refined the alignment by selecting all H.abietis-associated sequences and those of closely related bacterial type strains present in the SILVA database. The final alignment was produced by filtering some sequences: we only retained those sequences from the pine weevil that corresponded to the 50 most prevalent OTUs and in addition those of minor OTUs that clustered together with other bark beetle or weevil-associated bacterial sequences. We also eliminated those bacterial sequences from bark beetles and weevils (other than H. abietis) that did not cluster together with any other insect-associated bacteria in this study.Full raw 454 sequencing fasta fileRaw_454_Seqs.fnaFull raw 454 sequencing quality fileRaw_454_Seqs.qual