DOI: 10.5061/dryad.905qfttgb
title: Leaf data of 101 species, varieties, forms, and cultivars of bamboo

description: The data include four comma-delimited (CSV) files and one word
 document. data1.csv file saves the raw data (including
 blade fresh mass, dry mass, area, length, width, perimeter and
 other measures) of 10045 leaves from 101 species, varieties, forms, and
 cultivars of bamboo; data2.csv file saves the results of the leaf dry mass
 per unit area (g/m2) comparison among 101 data sets; data3.csv
 file saves the results of the quotient of blade width to
 length comparison among 101 data sets; data4.csv file
 saves the fitted results for the scaling relationships of blade dry mass
 vs. surface area for 101 data sets; the word document
 (i.e., the metadata.docx file) is the metadata, which is used to show the
 meanings of the column variables in each table.
description: Leaves from a total of 101
 species, varieties, forms, and cultivars of bamboo were collected.
 data1.csv file
 provides the scientific names [according to the Flora of China (Wu, Raven
 and Hong 2006) and <i>Bamboos of the World</i> (Ohrnberger
 1999)]. Leaves were randomly collected from different locations within
 middle canopies without distinguishing between sun or shade leaves
 during May-July of 2018. Samples were gathered from at least three
 different healthy adult individuals that were similar in size and age.
 Only mature leaves were collected and processed within 10 min in the
 field. Leaves were placed into large sealed polyethylene bags containing
 wet paper to maintain moisture. The bags were stored in an ice box to
 prevent decomposition even though the collection sites of most materials
 in Nanjing were near the laboratory, and the entire processing was
 completed within 2 h. Approximately 100 leaves were collected from
 different conspecifics for each taxon and measured to ascertain leaf area,
 width, length, fresh mass and dry mass. <i>W/L</i> and LMA
 were subsequently calculated. Leaf lamina
 area and shape were recorded using a HP Scanjet 4850 scanner
 (Hewlett-Packard Company, Palo Alto, California). The images were saved as
 bitmap images at a 200 dpi resolution. Adobe Photoshop (version: CC 2017)
 was used to obtain a new layer of the leaf edge in a black-white image
 (Shi <i>et al.</i> 2018). The M-function [using Matlab version
 ≥ 2009a developed by Shi <i>et al</i>. (2018) and Su et al.
 (2019)] was used to extract the planar coordinates of the leaf edge from
 each black-white image; the R script developed by Su <i>et
 al.</i> (2019) was then used to calculate leaf area, length, width,
 perimeter and other measures. Leaves were then dried in a ventilated oven
 at 80 °C for at least 72 h until obtaining a constant dry mass, which was
 measured using an electronic balance (ME204/02, Mettler Toledo Instrument
 (Shanghai) Co., Ltd, Shanghai, China). <strong>References:</strong> Ohrnberger D
 (1999) The bamboos of the world: Annotated nomenclature and literature of
 the species and the higher and lower taxa. Amsterdam: Elsevier.
 Shi P, Ratkowsky DA, Li Y, Zhang L, Lin S, Gielis J (2018) General leaf-area geometric formula exists for plants-Evidence from the simplified Gielis equation. Forests 9: 714 Su J, Niklas KJ, Huang W, Yu X, Yang Y, Shi P (2019) Lamina shape does not correlate with lamina surface area: An analysis based on the simplified Gielis equation. Glob Ecol Conserv 19: e00666 Wu Z, Raven PH, Hong D (2006) Flora of China (Vol. 22): Poaceae. Beijing, China: Science Press, and St. Louis, MO: Missouri Botanical Garden Press.