DOI: 10.5061/dryad.zgmsbcchr
title: Data for: Drought and temperature stresses impact pollen production and autonomous selfing in a California wildflower, Collinsia heterophylla

description: Ongoing climatic changes have altered growing conditions for plants by
 limiting water availability and inducing unprecedented temperature
 increases, eliciting plant functional responses that compromise floral
 trait expression, reduce pollen production but promote early
 self-pollination. In a controlled greenhouse study, plants of Collinsia
 heterophylla, an annual mixed-mated hermaphrodite, were grown under
 temperature and water stresses. Floral trait responses and related effects
 on plant reproductive success were recorded. Plants grown under
 temperature and water stresses were shorter and had fewer leaves at
 flowering than control plants. Temperature-stressed plants flowered
 earlier and had smaller flowers that produced fewer per capita pollen
 grains than control and water-stressed plants. While lifetime flower
 production in plants experiencing temperature stress alone or water stress
 alone was similar to control plants, those receiving combined temperature
 and water stresses had significantly lower lifetime flower production.
 Abiotic stress did not affect investment in female traits such as ovule
 number per flower but impacted male traits seen as fewer pollen grains per
 flower. In plants experiencing abiotic stress, an increase in autonomous
 self-pollination was facilitated through a compromise in herkogamy, a
 sexual interference mechanism to promote cross-pollination. Spatial
 separation between stigma and anther, a measure of herkogamy, was zero or
 negative in stressed plants. Thus, abiotic stress responses in plants did
 not compromise plant reproductive success as autonomous selfing was
 augmented. In nature, such responses will likely reduce available
 nutrition for pollinators through decreased flower and pollen production.
description: Dataset was collected by recording plant and floral traits by
 hand and collected data were entered into EXCEL worksheets for processing.
 IBM SPSS Statistics 21.0 (SPSS Inc. Chicago, IL) was used for statistical
 analyses.
description: The included data file can be opened with any spreadsheet
 software including MS Excel or Google Sheets.
description: # Arathi and Smith (2023), Drought and temperature stresses impact pollen
 production and autonomous selfing in a California wildflower, Collinsia
 heterophylla, Dryad, Dataset, Dryad, Dataset,
 --- The dataset contains
 floral trait measures and plant vegetative measures for the different
 abiotic stress treatments. The studies were conducted in a greenhouse
 growth chamber. There are no field sites associated with the data. Methods
 of data collection: see manuscript for details ## Description of the data
 and file structure The data are in a spreadsheet format saved as a
 CSV/text file. The column headers that describe the traits are
 self-explanatory. For specific terms only relevant to this study or
 species, the definitions are provided in the published manuscript. NA in
 the data file represents no data and should not be interpreted as 0.00.
 File count: 2 File format: .csv ## Details for: VWC data\_Arathi and
 Smith.csv Description: a comma-delimited file containing the soil water
 content (VWC) for all the treatments. The file can be reformatted for use
 with any other software. Format: .csv Size: 1 KB Dimensions: 47 rows x 4
 columns Variables: * trtnum: one-digit identification code for a treatment
 * trtname: treatment: ND = normal temperature, dry; NA: Normal
 temperature, adequate water; HA: High temperature, adequate water; HD:
 High temperature, dry * VWC: Soil water content in % * Treatment names ##
 Details for: Floral traits data\_Arathi and Smith.csv Description: a
 comma-delimited file containing the vegetative and reproductive trait
 measures for all the treatments. The file can be reformatted for use with
 any other software. Format: .csv Size: 80 KB Dimensions: 535 rows x 33
 columns Variables: * ID: two-digit alpha-numeric code for each replicate
 plant * gc: growth chamber 1 or 2 * trtnum: one-digit identification code
 for a treatment * Name: treatments in the study Control, heat, drought,
 Hot and dry * Main treatment: temperature effect: normal or high * sub
 treatment: watered or dry * DOF: Date of flowering * plht: plant height at
 flowering in cM * leafnum: leaf number at flowering * antherlen: length of
 anthers in each flower in mM * stylelen: length of style in each flower in
 mM * herkogamy: difference of style length and anther length in mM *
 avgherk: average herkogamy in mM * flowerlen: length of corolla from the
 base to the tip of the lower lip in mM * avgflowerlen: average of flower
 length in mM * flowerwid: width of flower in mM * avgflowerwid: average of
 flower width in mM * pollennum: total number of pollen grains per flower *
 pollenvia: proportion of viable pollen per flower * arcsinpollenviability:
 arc sin transformed value for proportion of viable pollen per flower *
 pollentubelen: length of pollen tubes in the culture in µM * seednum: seed
 numebr per fruit * VWC: volumetric water content of soil in % in each pot
 * flowernum: number of flowers per plant * fruitnum: number of fruits per
 plant * fruitabort: proportion of aborted fruits per plant * asinfruitab:
 arc sin transformed value for proportion of fruits aborted per plant *
 seedwtten: weight of ten seeds in mG * Seedabprop: proportion of seeds
 aborted * asinseedab: arc sin transformed value for proportion of seeds
 aborted * seedtoovule: ratio of seed number to ovule number *
 arcsinseedovule: arc sin transformed seed to ovule ratio