DOI: 10.15482/usda.adc/1529118
title: Data from: Identification of robust yield QTL derived from cultivated emmer for durum wheat improvement

description: Phenotypic Data
Two durum × cultivated emmer recombinant inbred line (RIL) populations were evaluated for grain yield components under field conditions in North Dakota, USA. The BP025 population was developed by crossing Ben (PI 596557), a North Dakota hard amber durum variety, with PI 41025, a cultivated emmer accession collected near Samara, Russia. The BP025 population consists of 200 RILs developed by single seed-descent and was advanced to the F7:8 generation. The RP883 population was developed by crossing the durum line Rusty (PI 639869) with PI 193883, a cultivated emmer wheat accession collected near Shewa, Ethiopia. The RP883 population consists of 190 RILs developed by single seed-descent and was advanced to the F7:8 generation.
The two populations were evaluated under field conditions in a total of three seasons for each and were grown in a randomized complete block design (RCBD) with three replicates per season. Plants were grown in hill plots with each plot consisting of 10-15 seeds and considered an experimental unit. The BP025 population was planted and evaluated in 2017, 2018, and 2019. The RP883 population was evaluated in 2018, 2019, and 2020. The 2017, 2018, and 2019 plots were grown at the North Dakota State University field site near Prosper, ND (47.002°N, 97.115°W), and the 2020 plots were grown at the NDSU agronomy seed farm near Casselton, ND (46.880°N, 97.243°W). The BP025 and RP883 populations and parental lines were evaluated for 11 traits including days to heading (DTH), plant height (PHT), the total number of spikelets per spike (SPS), kernels per spike (KPS), grain weight per spike (GWS), thousand kernel weight (TKW), kernel area (KA), kernel width (KW), kernel length (KL), kernel circularity (KC), and kernel length:width ratio (KLW). DTH was measured as the number of days from planting until 50 % of the spikes emerged completely beyond the flag leaf. PHT was measured in centimeters from the base of the plot to the tip of the tallest spike (excluding awns). Eight heads were used for phenotypic evaluations. SPS was counted as the total number of spikelets per spike. KPS, GWS, TKW, KA, KW, KL, KC, and KLW were obtained using a MARVIN grain analyzer (GAT Sensorik GMBH, Neubrandenburg, Germany). For KPS and GWS, the value obtained by the MARVIN for each sample was divided by the number of spikes in that sample to obtain KPS and GWS for data analysis.
Genotypic Data
DNA of the BP025 population was extracted and genotyped using the Illumina iSelect 9k wheat SNP array. The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from PI 41025, “B” represents an allele from Ben, and “-“ indicates missing data. The data was used to assemble the linkage-based genetic maps for the 14 durum wheat chromosomes and further used in statistical analysis to identify chromosome regions harboring genes associated with the various phenotypic traits mentioned in the phenotypic file.
DNA of the RP883 population was extracted and genotyped using the Illumina iSelect 90k wheat SNP array. The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from Rusty, “B” represents an allele from PI 193883, and “-“ indicates missing data. The data was used to assemble the linkage-based genetic maps for the 14 durum wheat chromosomes and further used in statistical analysis to identify chromosome regions harboring genes associated with the various phenotypic traits mentioned in the phenotypic file.
Resources in this dataset:


Resource Title: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population BP025
File Name: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population BP025.xlsx
Resource Description: The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from PI 41025, “B” represents an allele from Ben, and “-“ indicates missing data.


Resource Title: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population RP883
File Name: Genotypic data for the durum x cultivated emmer wheat recombinant inbred population RP883.xlsx
Resource Description: The genotypic data file consists of the marker names, the chromosome assignments of the markers, the linkage map position of the markers, and the genotypic calls for each marker within each RIL where “A” represents an allele from Rusty, “B” represents an allele from PI 193883, and “-“ indicates missing data.


Resource Title: Phenotypic data collected from the durum x cultivated emmer wheat recombinant inbred populations BP025 and RP883
File Name: Phenotypic data collected from the durum x cultivated emmer wheat recombinant inbred populations BP025 and RP883.xlsx
Resource Description: In the data file, column headings indicate the trait evaluated, the year, and replicate or average of all three replicates. For example, “SPS2017rep1” indicates rep 1 of the spikelets per spike trait collected in the 2017 trial. Sheet 1 consists of the BP025 population data, and sheet 2 consists of the RP883 population data. An entry of ‘NA’ indicates missing data.