cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.18419/darus-3765
title: D1244 sensor data (November 2023)

description: &lt;p&gt;
&lt;b&gt;General information:&lt;/b&gt;&lt;br&gt;
This dataset contains measurements from the adaptive high-rise demonstrator building D1244, built in the scope of the &lt;a href="https://www.sfb1244.uni-stuttgart.de/en/"&gt;CRC1244&lt;/a&gt;. This 36m high building is equipped with 24 hydraulic actuators providing the basis for its structural adaptation. Strain gauges, pressure sensors and position encoders are mounted throughout the building and used for state estimation and monitoring.
&lt;/p&gt;

&lt;p&gt;
&lt;b&gt;Structure of the dataset:&lt;/b&gt;&lt;br&gt;
 &lt;ul&gt;
 &lt;li&gt;Each zip-file contains measurements of one day in the hdf5 format.&lt;/li&gt;
 &lt;li&gt;The hdf5-files in each zip-file contain an array of 244 signals sampled over 10^6 time steps at approximately 200Hz.&lt;/li&gt;
 &lt;li&gt;&lt;code&gt;labels.csv&lt;/code&gt; contains auxiliary information on all measured signals, including the sensor type and the sensor's location in the building &lt;/li&gt;
&lt;/ul&gt; 
&lt;/p&gt;

&lt;p&gt;
&lt;b&gt;File contents:&lt;/b&gt;&lt;br&gt;
Each hdf5-file contains signals of the following types, arranged as stated in &lt;code&gt;labels.csv&lt;/code&gt;:
&lt;ul&gt;
 &lt;li&gt;&lt;b&gt;strain:&lt;/b&gt; strain (in mm/m) in columns and diagonal bracing elements, measured by strain gauges.&lt;/li&gt;
 &lt;li&gt;&lt;b&gt;pressure:&lt;/b&gt; pressure (in bar) in the piston side or the rod side chamber of a hydraulic actuator. For actuators in the diagonal bracing, the rod side chamber is permanently connected to the tank.&lt;/li&gt;
 &lt;li&gt;&lt;b&gt;posenc:&lt;/b&gt; displacement (in meters) of each actuator, measured by a position encoder. &lt;/li&gt;
 &lt;li&gt;&lt;b&gt;optic:&lt;/b&gt; optically measured displacement (in meters) of emitters attached to the building's facade.&lt;/li&gt;
&lt;/ul&gt;
The building consists of four modules spanning three stories each, and all sensors within a module are connected to a control cabinet, from which all measurements are transmitted. The cameras of the optical measurement system are placed outside the building and transmit their data separately from the sensors within the building. Therefore, there are an additional two signals per module (or camera):
&lt;ul&gt;
 &lt;li&gt;&lt;b&gt;timestamp:&lt;/b&gt; unix timestamp (seconds since 1st January 1970) of the control cabinet&lt;/li&gt;
 &lt;li&gt;&lt;b&gt;numvars:&lt;/b&gt; number of measured variables&lt;/li&gt;
&lt;/ul&gt;
Missing measurements are marked as &lt;i&gt;NaN&lt;/i&gt;. The optical measurement system is currently undergoing maintenance, which is why the corresponding signals are all NaN.
&lt;/p&gt;
description: D1244 sensor data
DOI: 10.18419/darus-3765/29
title: labels.tab

description: information on the measured variables including sensor type and location in the building
DOI: 10.18419/darus-1037/1
title: 00001137.JPG
DOI: 10.18419/darus-1037/2
title: 00001127.JPG
DOI: 10.18419/darus-1037/3
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title: 00001140.JPG
DOI: 10.18419/darus-1037/5
title: 00001132.JPG
DOI: 10.18419/darus-1037/6
title: 00001143.JPG
DOI: 10.18419/darus-1037/7
title: 00001148.JPG
DOI: 10.18419/darus-1037/8
title: 00001144.JPG
DOI: 10.18419/darus-1037/9
title: 00001141.JPG
DOI: 10.18419/darus-1037/10
title: 00001121.JPG
DOI: 10.18419/darus-1037/11
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DOI: 10.18419/darus-1037/15
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DOI: 10.18419/darus-1037/16
title: 00001142.JPG
DOI: 10.18419/darus-1037/17
title: 00001126.JPG
DOI: 10.18419/darus-1037/18
title: 00001139.JPG
DOI: 10.18419/darus-1037/19
title: 00001146.JPG
DOI: 10.18419/darus-1037/20
title: 00001154.JPG
DOI: 10.18419/darus-1037/21
title: 00001128.JPG
DOI: 10.18419/darus-1037/22
title: 00001122.JPG
DOI: 10.18419/darus-1037/23
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DOI: 10.18419/darus-1037/24
title: 00001134.JPG
DOI: 10.18419/darus-1037/25
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DOI: 10.18419/darus-1037/26
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DOI: 10.18419/darus-1037/27
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DOI: 10.18419/darus-1037/28
title: 00001135.JPG
DOI: 10.18419/darus-1037/29
title: 00001136.JPG
DOI: 10.18419/darus-1037/30
title: 00001150.JPG
DOI: 10.18419/darus-1037
title: 2D pictures of KK34-20

description: &lt;p&gt;Dataset contains all pictures (.jpg; .arw) that were taken during the &lt;a href="https://darus.uni-stuttgart.de/file.xhtml?fileId=49056&version=DRAFT"&gt;2D digitisation strategy&lt;/a&gt; for this object.&lt;/p&gt; &lt;p&gt;Order of data: object was digitised according to a digitisation schema visible within the Dataset&lt;/p&gt;&lt;ul&gt;&lt;li&gt;characteristic view,&lt;/li&gt; &lt;li&gt;front view,&lt;/li&gt; &lt;li&gt;left view,&lt;/li&gt; &lt;li&gt;back view,&lt;/li&gt; &lt;li&gt;right view,&lt;/li&gt;&lt;li&gt;bottom view,&lt;/li&gt; &lt;li&gt;upward view, &lt;/li&gt;&lt;li&gt;details (details 1, details 2, ..., details n)&lt;/li&gt;&lt;/ul&gt;&lt;p&gt; This schema is indicated within the pictures by inventory label and then labels according to view.&lt;/p&gt;&lt;p&gt; Further information one can find in the document: Setting and workflow for basic development of 2D.&lt;/p&gt;&lt;p&gt;This dataset is part of the digital gyroscope collection. For more information about 
the collection and its creation, please see the documentation dataset:
&lt;i&gt;Niklaus, Maria, 2021, "Information about the Gyrolog data repository", 
&lt;a href="https://doi.org/10.18419/darus-821"&gt;doi: 10.18419/darus-821&lt;/a&gt;, DaRUS.&lt;/i&gt;&lt;/p&gt;&lt;p&gt;&lt;b&gt;To refer to the whole collection: Wagner, Jörg F.; Ceranski, Beate; Fritsch, Dieter; Mammadov, Gasim; Niklaus, Maria; Schweizer, Timo; Simon, Sven; Zhan, Kun, 2021, "Digital Gyroscope Collection Created by the Project 'Gyrolog'", &lt;a href="https://doi.org/10.18419/darus-gyrolog"&gt;doi: 10.18419/darus-gyrolog&lt;/a&gt;, DaRUS.&lt;/b&gt;&lt;/p&gt;
description: directional gyro
DOI: 10.18419/darus-1037/31
title: 00001120.JPG
DOI: 10.18419/darus-1060/1
title: 00001179.JPG
DOI: 10.18419/darus-1060/2
title: 00001172.JPG
DOI: 10.18419/darus-1060/3
title: 00001177.JPG
DOI: 10.18419/darus-1060/4
title: 00001182.JPG
DOI: 10.18419/darus-1060/5
title: 00001170.JPG
DOI: 10.18419/darus-1060/6
title: 00001167.JPG
DOI: 10.18419/darus-1060/7
title: 00001159.JPG
DOI: 10.18419/darus-1060/8
title: 00001187.JPG
DOI: 10.18419/darus-1060/9
title: 00001183.JPG
DOI: 10.18419/darus-1060/10
title: 00001160.JPG
DOI: 10.18419/darus-1060/11
title: 00001171.JPG
DOI: 10.18419/darus-1060/12
title: 00001188.JPG
DOI: 10.18419/darus-1060/13
title: 00001162.JPG
DOI: 10.18419/darus-1060/14
title: 00001156.JPG
DOI: 10.18419/darus-1060/15
title: 00001163.JPG
DOI: 10.18419/darus-1060/16
title: 00001192.JPG
DOI: 10.18419/darus-1060/17
title: 00001161.JPG
DOI: 10.18419/darus-1060/18
title: 00001158.JPG
DOI: 10.18419/darus-1060/19
title: 00001164.JPG
DOI: 10.18419/darus-1060/20
title: 00001178.JPG
DOI: 10.18419/darus-1060/21
title: 00001157.JPG
DOI: 10.18419/darus-1060/22
title: 00001165.JPG
DOI: 10.18419/darus-1060/23
title: 00001189.JPG
DOI: 10.18419/darus-1060/24
title: 00001166.JPG
DOI: 10.18419/darus-1060/25
title: 00001180.JPG
DOI: 10.18419/darus-1060/26
title: 00001181.JPG
DOI: 10.18419/darus-1060/27
title: 00001169.JPG
DOI: 10.18419/darus-1060/28
title: 00001190.JPG
DOI: 10.18419/darus-1060/29
title: 00001191.JPG
DOI: 10.18419/darus-1060/30
title: 00001155.JPG
DOI: 10.18419/darus-1060
title: 2D pictures of PL06-20

description: &lt;p&gt;Dataset contains all pictures (.jpg; .arw) that were taken during the &lt;a href="https://darus.uni-stuttgart.de/file.xhtml?fileId=49056&version=DRAFT"&gt;2D digitisation strategy&lt;/a&gt; for this object.&lt;/p&gt; &lt;p&gt;Order of data: object was digitised according to a digitisation schema visible within the Dataset&lt;/p&gt;&lt;ul&gt;&lt;li&gt;characteristic view,&lt;/li&gt; &lt;li&gt;front view,&lt;/li&gt; &lt;li&gt;left view,&lt;/li&gt; &lt;li&gt;back view,&lt;/li&gt; &lt;li&gt;right view,&lt;/li&gt;&lt;li&gt;bottom view,&lt;/li&gt; &lt;li&gt;upward view, &lt;/li&gt;&lt;li&gt;details (details 1, details 2, ..., details n)&lt;/li&gt;&lt;/ul&gt;&lt;p&gt; This schema is indicated within the pictures by inventory label and then labels according to view.&lt;/p&gt;&lt;p&gt; Further information one can find in the document: Setting and workflow for basic development of 2D.&lt;/p&gt;&lt;p&gt;This dataset is part of the digital gyroscope collection. For more information about 
the collection and its creation, please see the documentation dataset:
&lt;i&gt;Niklaus, Maria, 2021, "Information about the Gyrolog data repository", 
&lt;a href="https://doi.org/10.18419/darus-821"&gt;doi: 10.18419/darus-821&lt;/a&gt;, DaRUS.&lt;/i&gt;&lt;/p&gt;&lt;p&gt;&lt;b&gt;To refer to the whole collection: Wagner, Jörg F.; Ceranski, Beate; Fritsch, Dieter; Mammadov, Gasim; Niklaus, Maria; Schweizer, Timo; Simon, Sven; Zhan, Kun, 2021, "Digital Gyroscope Collection Created by the Project 'Gyrolog'", &lt;a href="https://doi.org/10.18419/darus-gyrolog"&gt;doi: 10.18419/darus-gyrolog&lt;/a&gt;, DaRUS.&lt;/b&gt;&lt;/p&gt;
description: inertial platforms
DOI: 10.18419/darus-1060/31
title: 00001176.JPG
DOI: 10.18419/darus-3338/10
title: PHI_Physi_Na,K-PHI_ls_calc_15.pdf

description: CO2-Physisorption of Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/11
title: PHI_Physi_Na,K-PHI_hs_a.s._90%.pdf

description: CO2-Physisorption of Na,K-PHI_hs_a.s._90%
DOI: 10.18419/darus-3338/12
title: PHI_Physisorption_CO2.xlsx

description: Collection of all CO2-Physisorption-data
DOI: 10.18419/darus-3338/13
title: PHI_ICP.xlsx

description: Raw Data of ICP-Data
DOI: 10.18419/darus-3338/4
title: PHI_XRD_Raw_Na,K-PHI_hs_calc_16.UXD

description: XRD-raw-data of Na,K-PHI_hs_calc_16%
DOI: 10.18419/darus-3338/5
title: PHI_XRD_Raw_Na,K-PHI_hs_a.s._N2_39.UXD

description: XRD-raw-data of Na,K-PHI_hs_a.s._N2_39%
DOI: 10.18419/darus-3338/6
title: PHI_XRD_Raw_Na,K-PHI_hs_a.s._90.UXD

description: XRD-raw-data of Na,K-PHI_hs_a.s._90%
DOI: 10.18419/darus-3338/9
title: PHI_Physi_Na,K-PHI_hs_calc_16%.pdf

description: CO2-Physisorption of Na,K-PHI_hs_calc_16%
DOI: 10.18419/darus-3338/1
title: PHI_XRD.xlsx

description: collection of all XRD-data
DOI: 10.18419/darus-3338/14
title: Na,K-PHI_hs_calc_16_10ym.tif

description: SEM Image of Na,K-PHI_hs_calc_16%
DOI: 10.18419/darus-3338/2
title: PHI_XRD_Raw_Na,K-PHI_ls_calc_15.UXD

description: XRD-raw-data of Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/3
title: PHI_XRD_Raw_Na,K-PHI_ls_a.s._100.UXD

description: XRD-raw-data of Na,K-PHI_ls_a.s._100%
DOI: 10.18419/darus-3338/7
title: PHI_XRD_Raw_Na,K-PHI_ls_calc_steamed_14.UXD

description: XRD-raw-data of Na,K-PHI_ls_calc_steamed_14%
DOI: 10.18419/darus-3338/8
title: PHI_XRD_Raw_Na,K-PHI_ls_N2_15.UXD

description: XRD-raw-data of Na,K-PHI_ls_N2_15%
DOI: 10.18419/darus-3338/15
title: Na,K-PHI_hs_calc_16_1ym.tif

description: SEM Image of Na,K-PHI_hs_calc_16%
DOI: 10.18419/darus-3338/16
title: Na,K-PHI_hs_a.s._90_10ym.tif

description: SEM Image of Na,K-PHI_hs_a.s._90%
DOI: 10.18419/darus-3338/17
title: Na,K-PHI_ls_calc_15_1ym.tif

description: SEM Image of Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/18
title: Na,K-PHI_ls_calc_15_10ym.tif

description: SEM Image of Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/19
title: Na,K-PHI_hs_a.s._90_1ym.tif

description: SEM Image of Na,K-PHI_hs_a.s._90%
DOI: 10.18419/darus-3338/20
title: PHI_27Al-MAS-NMR.xlsx

description: collection of all 27 Al MAS NMR Data
DOI: 10.18419/darus-3338/21
title: PHI_catalysis_all.xlsx

description: collection of catalysis data
DOI: 10.18419/darus-3338/22
title: PHI_27Al-MAS-NMR_Na,K-PHI_ls_calc_15%.zip

description: 27Al MAS NMR data for Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/23
title: PHI_27Al-MAS-NMR_Na,K-PHI_ls_calc_steamed_14%.zip

description: 27Al MAS NMR data for Na,K-PHI_ls_calc_steamed_14%
DOI: 10.18419/darus-3338/24
title: PHI_27Al-MAS-NMR_Na,K-PHI_hs_a.s._N2_39%.zip

description: 27Al MAS NMR data for Na,K-PHI_hs_a.s._N2_39%
DOI: 10.18419/darus-3338/25
title: PHI_27Al-MAS-NMR_Na,K-PHI_hs_a.s._90%.zip

description: 27Al MAS NMR data for Na,K-PHI_hs_a.s._90%
DOI: 10.18419/darus-3338/26
title: PHI_27Al-MAS-NMR_Na,K-PHI_hs_calc_16%.zip

description: 27Al MAS NMR data for Na,K-PHI_hs_calc_16%
DOI: 10.18419/darus-3338/27
title: GC_Na,K-PHI_ls_calc_15%_b.zip

description: GC data for Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/28
title: GC_Na,K-PHI_hs_calc_16.zip

description: GC data forNa,K-PHI_hs_calc_16
DOI: 10.18419/darus-3338/29
title: GC_Na,K-PHI_ls_calc_steamed_14%.zip

description: GC data for Na,K-PHI_ls_calc_steamed_14%
DOI: 10.18419/darus-3338
title: Data for: Influence of the Synthesis Protocol on the Catalytic Performance of PHI-Type Zeolites for the Dehydration of Lactic Acid

description: &lt;p&gt;This dataset encludes all gas chromatography (GC), &lt;sup&gt;27&lt;/sup&gt;Al magic angle spinning nuclear magnetic resonance spectroscopy (&lt;sup&gt;27&lt;/sup&gt;Al MAS NMR), x-ray diffraction (XRD), CO&lt;sub&gt;2&lt;/sub&gt; physisorption, inductively coupled plasma optical emission spectroscopy (ICP-OES) data and scanning electron microscope (SEM) images of the related publication: "Influence of the Synthesis Protocol on the Catalytic Performance of PHI-Type Zeolites for the Dehydration of Lactic Acid".&lt;/p&gt;

&lt;p&gt;The manuscript describes the influence of the synthesis protocol on the catalytic performance of PHI-type zeolites for the dehydration of lactic acid. Two PHI-type zeolites were synthesized with a few variations in the synthesis procedure. The catalysts were characterized and tested in the lactic acid dehydration reaction. During the catalytic reaction, a big difference in the performance was observed.&lt;/p&gt;

&lt;p&gt;The data used for the manuscript are sorted by the respective characterization method. The data are named according to the sample names. The raw data can be processed with the corresponding software.&lt;/p&gt;
description: ASiQwin, 3.01
description: TopSpin, 3.6.2
description: Adobe Photoshop, CS2 6.2
description: Agilent Chemstation, null
description: ChemDraw, 19.0.0.22
DOI: 10.18419/darus-3338/30
title: GC_Na,K-PHI_ls_calc_15%_a.zip

description: GC data for Na,K-PHI_ls_calc_15%
DOI: 10.18419/darus-3338/31
title: GC_Na,K-PHI_hs_a.s._90%.zip

description: GC data for Na,K-PHI_hs_a.s._90%
DOI: 10.18419/darus-4301/8
title: 22_0_character.nc

description: Theory data for 22(0), which gives the amount of f and g character of each state (0-1).
DOI: 10.18419/darus-4301/1
title: eval.py
DOI: 10.18419/darus-4301/3
title: 32_0.nc

description: Theory data for 32(0) with M_N = 3 (Stark map theory data).
DOI: 10.18419/darus-4301/5
title: 22_0_M_N_3.nc

description: Theory data for 22(0) with M_N = 3.
DOI: 10.18419/darus-4301/7
title: 22_0_M_N_4.nc

description: Theory data for 22(0) with M_N = 4.
DOI: 10.18419/darus-4301/4
title: __init__.py
DOI: 10.18419/darus-4301/6
title: __init__.py
DOI: 10.18419/darus-4301/9
title: figure_4_character.py
DOI: 10.18419/darus-4301/2
title: toc.py
DOI: 10.18419/darus-4301/10
title: main.py
DOI: 10.18419/darus-4301/11
title: 22_0.nc

description: Theory data for 22(0) with M_N = 3 (Stark map theory data).
DOI: 10.18419/darus-4301/12
title: config.py
DOI: 10.18419/darus-4301/13
title: _mplsetup.py
DOI: 10.18419/darus-4301/14
title: starkutils.py
DOI: 10.18419/darus-4301/15
title: 22_0_M_N_1.nc

description: Theory data for 22(0) with M_N = 1.
DOI: 10.18419/darus-4301/16
title: requirements.txt
DOI: 10.18419/darus-4301/17
title: 22_0_M_N_2.nc

description: Theory data for 22(0) with M_N = 2.
DOI: 10.18419/darus-4301/18
title: __init__.py
DOI: 10.18419/darus-4301/19
title: 22_0_M_N_0.nc

description: Theory data for 22(0) with M_N = 0.
DOI: 10.18419/darus-4301/20
title: figure_2_dual_plot.py
DOI: 10.18419/darus-4301/21
title: _macros.py
DOI: 10.18419/darus-4301/22
title: lockinConversions.py
DOI: 10.18419/darus-4301/23
title: figure_3_high_field.py
DOI: 10.18419/darus-4301/24
title: 22_0.h5

description: Measurement data for 22(0) state at low fields (about -10V/cm to 10V/cm)
DOI: 10.18419/darus-4301/25
title: paths.py
DOI: 10.18419/darus-4301/26
title: 22_0_non_cut.h5

description: Measurement data for 22(0) state at low fields (about -10V/cm to 10V/cm), where the range is still set to full measurement.
DOI: 10.18419/darus-4301/27
title: 32_0.h5

description: Measurement data for 32(0) state at low fields (about -10V/cm to 10V/cm)
DOI: 10.18419/darus-4301/28
title: 22_0.h5

description: Measurement data for 22(0) state at high fields (about -35V/cm to 35V/cm)
DOI: 10.18419/darus-4301
title: Replication Data for: High-resolution cw laser spectroscopy of long-lived Rydberg states in NO

description: This dataset holds all measurement data, as well as the evaluation and plotting scripts to replicate all figures of the paper. Please refer to the &lt;a href="https://darus.uni-stuttgart.de/file.xhtml?fileId=301148"&gt;README.md&lt;/a&gt; file for more information.
DOI: 10.18419/darus-4301/29
title: README.md
DOI: 10.18419/darus-815/11
title: 3B K777A 1 µM CN.txt
DOI: 10.18419/darus-815/9
title: 3B N652A 2 µM CN.txt
DOI: 10.18419/darus-815/12
title: 3B N658A 6 µM CN.txt
DOI: 10.18419/darus-815/2
title: 3B N779A 0.5 µM CN.txt
DOI: 10.18419/darus-815/3
title: 3B N656D 10 µM CN.txt
DOI: 10.18419/darus-815/5
title: 3B R823A 1.5 µM CN.txt
DOI: 10.18419/darus-815/6
title: 3B N779D 1 µM CN.txt
DOI: 10.18419/darus-815/7
title: 3B R661A 1.5 µM CN.txt
DOI: 10.18419/darus-815/8
title: 3B N656D 5 µM CN.txt
DOI: 10.18419/darus-815/10
title: 3B V657A 1 µM CN.txt
DOI: 10.18419/darus-815/13
title: 3B N656A 1.0 µM CN.txt
DOI: 10.18419/darus-815/4
title: 3B V657A 2 µM CN.txt
DOI: 10.18419/darus-815/14
title: 3B T775A 5 µM CN.txt
DOI: 10.18419/darus-815/15
title: 3B N652A 1 µM CN.txt
DOI: 10.18419/darus-815/16
title: 3B T775A 10 µM CN.txt
DOI: 10.18419/darus-815/17
title: 3B N779D 0.5 µM CN.txt
DOI: 10.18419/darus-815/18
title: 3B WT 1.0 µM CN.txt
DOI: 10.18419/darus-815/19
title: 3B R823A 3.0 µM CN.txt
DOI: 10.18419/darus-815/20
title: 3B N779A 1.0 µM CN.txt
DOI: 10.18419/darus-815/21
title: 3B R661A 3.0 µM CN.txt
DOI: 10.18419/darus-815/22
title: 3B WT 0.5 µM CN.txt
DOI: 10.18419/darus-815/23
title: 3B N658A 3 µM CN.txt
DOI: 10.18419/darus-815/24
title: 3B N656A 0.5 µM CN.txt
DOI: 10.18419/darus-815/25
title: readme.pdf
DOI: 10.18419/darus-815/26
title: 3B K777A 2 µM CN.txt
DOI: 10.18419/darus-815/27
title: no enzyme CN.txt
DOI: 10.18419/darus-815/28
title: h3B 1 µM HM.txt
DOI: 10.18419/darus-815/29
title: R823A 3 µM HM.txt
DOI: 10.18419/darus-815/30
title: h3B 0.5 µM HM.txt
DOI: 10.18419/darus-815
title: Deep enzymology data related to Dukatz et al.: Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase.

description: Experimental procedures: Libraries of double stranded DNA substrates with CpG, CpH or CpN sites in randomized sequence context were methlyated by DNMT3B. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA was digested with the BsaI-HFv2 enzyme and a hairpin was ligated using T4 DNA ligase (NEB). The DNA was bisulfite converted using EZ DNA Methylation-Lightning kit (ZYMO RESEARCH) according to the manufacturer protocol, purified and eluted with 10 µL ddH2O. Libraries for Illumina Next Generation Sequencing (NGS) were produced with the two-step PCR approach. In the first PCR, 2 µL of bisulfite-converted DNA were amplified with the HotStartTaq DNA Polymerase (QIAGEN) and primers containing internal barcodes using following conditions: 15 min at 95 °C, 10 cycles of 30 sec at 94 °C, 30 sec at 50 °C, 1 min and 30 sec at 72 °C, and final 5 min at 72 °C; using a mixture containing 1x PCR Buffer, 1x Q-Solution, 0.2 mM dNTPs, 0.05 U/µL HotStartTaq DNA Polymerase, 0.4 µM forward and 0.4 µM reverse primers in a total volume of 20 µL. In the second PCR, 1 µL of obtained products were amplified by Phusion Polymerase (Thermo) with another set of primers to introduce adapters and indices needed for NGS (30 sec at 98 °C, 10 cycles - 10 sec at 98 °C, 40 sec at 72 °C, and 5 min at 72 °C). PCRII was carried out in 1x Phusion HF Buffer, 0.2 mM dNTPs, 0.02 U/µL Phusion HF DNA Polymerase, 0.4 µM forward and 0.4 µM reverse primers in a total volume of 20 µL. Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter).
description: PMID 33105482
DOI: 10.18419/darus-815/31
title: readme.pdf
DOI: 10.18419/darus-947/1
title: 00004239.JPG
DOI: 10.18419/darus-947/2
title: 00004240.JPG
DOI: 10.18419/darus-947/3
title: 00004235.JPG
DOI: 10.18419/darus-947/4
title: 00004236.JPG
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title: 00004247.JPG
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title: 00004241.JPG
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title: 00004238.JPG
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title: 00004242.JPG
DOI: 10.18419/darus-947/9
title: 00004237.JPG
DOI: 10.18419/darus-947/10
title: 00004244.JPG
DOI: 10.18419/darus-947/11
title: 00004246.JPG
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title: 00004245.JPG
DOI: 10.18419/darus-947/13
title: 00004243.JPG
DOI: 10.18419/darus-947/14
title: 00004217.JPG
DOI: 10.18419/darus-947/15
title: 00004248.JPG
DOI: 10.18419/darus-947/16
title: 00004203.JPG
DOI: 10.18419/darus-947/17
title: 00004206.JPG
DOI: 10.18419/darus-947/18
title: 00004215.JPG
DOI: 10.18419/darus-947/19
title: 00004208.JPG
DOI: 10.18419/darus-947/20
title: 00004204.JPG
DOI: 10.18419/darus-947/21
title: 00004207.JPG
DOI: 10.18419/darus-947/22
title: 00004214.JPG
DOI: 10.18419/darus-947/23
title: 00004213.JPG
DOI: 10.18419/darus-947/24
title: 00004212.JPG
DOI: 10.18419/darus-947/25
title: 00004211.JPG
DOI: 10.18419/darus-947/26
title: 00004216.JPG
DOI: 10.18419/darus-947/27
title: 00004209.JPG
DOI: 10.18419/darus-947/28
title: 00004205.JPG
DOI: 10.18419/darus-947/29
title: 00004210.JPG
DOI: 10.18419/darus-947
title: 2D pictures of WKI04/1-20

description: &lt;p&gt;Dataset contains all pictures (.jpg) that were taken during the &lt;a href="https://darus.uni-stuttgart.de/file.xhtml?fileId=49056&version=DRAFT"&gt;2D digitisation strategy&lt;/a&gt; for this object.&lt;/p&gt; &lt;p&gt;Order of data: object was digitised according to a digitisation schema visible within the Dataset&lt;/p&gt;&lt;ul&gt;&lt;li&gt;characteristic view,&lt;/li&gt; &lt;li&gt;front view,&lt;/li&gt; &lt;li&gt;left view,&lt;/li&gt; &lt;li&gt;back view,&lt;/li&gt; &lt;li&gt;right view,&lt;/li&gt;&lt;li&gt;bottom view,&lt;/li&gt; &lt;li&gt;upward view, &lt;/li&gt;&lt;li&gt;details (details 1, details 2, ..., details n)&lt;/li&gt;&lt;/ul&gt;&lt;p&gt; This schema is indicated within the pictures by inventory label and then labels according to view.&lt;/p&gt;&lt;p&gt; Further information one can find in the document: Setting and workflow for basic development of 2D.&lt;/p&gt;&lt;p&gt;This dataset is part of the digital gyroscope collection. For more information about 
the collection and its creation, please see the documentation dataset:
&lt;i&gt;Niklaus, Maria, 2021, "Information about the Gyrolog data repository", 
&lt;a href="https://doi.org/10.18419/darus-821"&gt;doi: 10.18419/darus-821&lt;/a&gt;, DaRUS.&lt;/i&gt;&lt;/p&gt;&lt;p&gt;&lt;b&gt;To refer to the whole collection: Wagner, Jörg F.; Ceranski, Beate; Fritsch, Dieter; Mammadov, Gasim; Niklaus, Maria; Schweizer, Timo; Simon, Sven; Zhan, Kun, 2021, "Digital Gyroscope Collection Created by the Project 'Gyrolog'", &lt;a href="https://doi.org/10.18419/darus-gyrolog"&gt;doi: 10.18419/darus-gyrolog&lt;/a&gt;, DaRUS.&lt;/b&gt;&lt;/p&gt;
description: rate integrating gyro
DOI: 10.18419/darus-947/30
title: 00004188.JPG