DOI: 10.5061/dryad.pk0p2ngxn
title: Data from: Increased susceptibility of WHIM mice to Papillomavirus-induced disease is dependent upon immune cell dysfunction

description: Warts, Hypogammaglobulinemia, Infections, and Myelokathexis (WHIM)
 syndrome is a rare primary immunodeficiency disease in humans caused by a
 gain of function in CXCR4, mostly due to inherited heterozygous mutations
 in CXCR4. One major clinical symptom of WHIM patients is their high
 susceptibility to human papillomavirus (HPV) induced disease, such as
 warts. Persistent high risk HPV infections cause 5% of all human cancers,
 including cervical, anogenital, head and neck and some skin cancers. WHIM
 mice bearing the same mutation identified in WHIM patients were created to
 study the underlying causes for the symptoms manifest in patients
 suffering from the WHIM syndrome. Using murine papillomavirus (MmuPV1) as
 an infection model in mice for HPV-induced disease, we demonstrate that
 WHIM mice are more susceptible to MmuPV1-induced warts (papillomas)
 compared to wild type mice. Namely, the incidence of papillomas is higher
 in WHIM mice compared to wild type mice when mice are exposed to low doses
 of MmuPV1. MmuPV1 infection facilitated both myeloid and lymphoid cell
 mobilization in the blood of wild type mice but not in WHIM mice. Higher
 incidence and larger size of papillomas in WHIM mice correlated with lower
 abundance of infiltrating T cells within the papillomas. Finally, we
 demonstrate that transplantation of bone marrow from wild type mice into
 WHIM mice normalized the incidence and size of papillomas, consistent with
 the WHIM mutation in hematopoietic cells contributing to higher
 susceptibility of WHIM mice to MmuPV1-induced disease. Our results provide
 evidence that MmuPV1 infection in WHIM mice is a powerful preclinical
 infectious model to investigate treatment options for alleviating
 papillomavirus infections in WHIM syndrome.
description: # Data from: Increased susceptibility of WHIM mice to
 Papillomavirus-induced disease is dependent upon immune cell dysfunction
 [https://doi.org/10.5061/dryad.pk0p2ngxn](https://doi.org/10.5061/dryad.pk0p2ngxn) This data set contains all raw data points (in different formats) used to construct the points in figures in the manuscript. Different software/programs may be used for statistical and figure purposes. Details about softwares/programs used for each figure/subfigure are described below in Code/Software section. ## Description of the data and file structure Figure 1A: Data in Contingency table format (shown as frequency), groups (genotype and infected virus dose), outcomes (papilloma or none). File format Prism Graphpad. Figure 1B: Data in Column format, groups (genotype), values (papilloma size). File format Prism Graphpad. Figure 1D: Data in Contingency table format (shown as fraction of total), groups (genotype), outcome (Normal, Mild Dysplasia, Moderate Dysplasia, Severe Dysplasia or Cancer). File format Prism Graphpad. Figure 1E and 1F: Data in Column format, groups (genotype), values (papilloma size). File format Prism Graphpad. Figure 2A: Data in Grouped format, time points for data collection (pre-infection[pre] and 4 weeks post infection [4wk PI]), groups (genotype). File format Prism Graphpad. Figure 2B and 2D: Data in Column format, groups (genotype), values (percentage of total live cells for each cell type). File format Prism Graphpad. Figure 2C: Data in Column format, groups (genotype), values (number of cell counts in red K14+ area). File format Prism Graphpad. Figure 3B and 3C: Data in Column format, groups (BM donor genotype), values (cell number per microliter of blood for each cell type). File format Prism Graphpad. Figure 3D: Data in Contingency table format (shown as frequency), groups (BM donor genotype), outcomes (papilloma or none). File format Prism Graphpad. Figure 3E: Data in Column format, groups (BM donor genotype), values (papilloma size). File format Prism Graphpad. Figure 3F: Data in Parts of whole (Pie chart) format, parts (persistence or complete regression). File format Prism Graphpad. Figure 3G: Data in Column format, groups (BM donor genotype), values (percentage of total live cells for each cell type). File format Prism Graphpad. Figure 4B and 4C: Data in Column format, groups (recipient genotype), values (cell number per microliter of blood for each cell type). File format Prism Graphpad. Figure 4D: Data in Contingency table format (shown as frequency), groups (recipient genotype), outcomes (papilloma or none). File format Prism Graphpad. Figure 4E: Data in Column format, groups (recipient genotype), values (papilloma size). File format Prism Graphpad. Figure 4F: Data in Column format, groups (recipient genotype), values (percentage of total live cells for each cell type). File format Prism Graphpad. ## Sharing/Access information There is no other ways to access the data or other sources the data is derived from. ## Code/Software Prism graphpad is used to run .prism .pzfx files. All statistical analyses are performed with existing programs within Prism grapgpad. All statistical analyses runs and results are included within the prism file for each subfigure. Image preview is used to run TIFF files.