cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19904155
title: P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis

description: Abstract Background: Snake venoms are composed of pharmacologically active proteins that are evolutionarily diverse, stable and specific to targets. Hence, venoms have been explored as a source of bioactive molecules in treating numerous diseases. Recent evidences suggest that snake venom proteins may affect the formation of new blood vessels. Excessive angiogenesis has been implicated in several pathologies including tumours, diabetic retinopathy, arthritis, inter alia. In the present study, we have examined the effects of P-I metalloproteinases isolated from Bothrops moojeni (BmMP-1) and Bothrops atrox (BaMP-1) and L-amino acid oxidases (LAAO) isolated from B. moojeni (BmLAAO) and B. atrox (BaLAAO) on biochemical and functional aspects of angiogenesis. Methods: P-I metalloproteinases and LAAO were purified from venom by molecular size exclusion and ion-exchange chromatography and subsequently confirmed using mass spectrometry. The P-I metalloproteinases were characterized by azocaseinolytic, fibrinogenolytic and gelatinase activity and LAAO activity was assessed by enzyme activity on L-amino acids. Influence of these proteins on apoptosis and cell cycle in endothelial cells was analysed by flow cytometry. The angiogenic activity was determined by in vitro 3D spheroid assay, Matrigel tube forming assay, and in vivo agarose plug transformation in mice. Results: P-I metalloproteinases exhibited azocaseinolytic activity, cleaved α and partially β chain of fibrinogen, and displayed catalytic activity on gelatin. LAAO showed differential activity on L-amino acids. Flow cytometry analysis indicated that both P-I metalloproteinases and LAAO arrested the cells in G0/G1 phase and further induced both necrosis and apoptosis in endothelial cells. In vitro, P-I metalloproteinases and LAAO exhibited significant anti-angiogenic properties in 3D spheroid and Matrigel models by reducing sprout outgrowth and tube formation. Using agarose plug transplants in mice harbouring P-I metalloproteinases and LAAO we demonstrated a marked disruption of vasculature at the periphery. Conclusion: Our research suggests that P-I metalloproteinases and LAAO exhibit anti-angiogenic properties in vitro and in vivo.
DOI: 10.6084/m9.figshare.19904155.v1
title: P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis

description: Abstract Background: Snake venoms are composed of pharmacologically active proteins that are evolutionarily diverse, stable and specific to targets. Hence, venoms have been explored as a source of bioactive molecules in treating numerous diseases. Recent evidences suggest that snake venom proteins may affect the formation of new blood vessels. Excessive angiogenesis has been implicated in several pathologies including tumours, diabetic retinopathy, arthritis, inter alia. In the present study, we have examined the effects of P-I metalloproteinases isolated from Bothrops moojeni (BmMP-1) and Bothrops atrox (BaMP-1) and L-amino acid oxidases (LAAO) isolated from B. moojeni (BmLAAO) and B. atrox (BaLAAO) on biochemical and functional aspects of angiogenesis. Methods: P-I metalloproteinases and LAAO were purified from venom by molecular size exclusion and ion-exchange chromatography and subsequently confirmed using mass spectrometry. The P-I metalloproteinases were characterized by azocaseinolytic, fibrinogenolytic and gelatinase activity and LAAO activity was assessed by enzyme activity on L-amino acids. Influence of these proteins on apoptosis and cell cycle in endothelial cells was analysed by flow cytometry. The angiogenic activity was determined by in vitro 3D spheroid assay, Matrigel tube forming assay, and in vivo agarose plug transformation in mice. Results: P-I metalloproteinases exhibited azocaseinolytic activity, cleaved α and partially β chain of fibrinogen, and displayed catalytic activity on gelatin. LAAO showed differential activity on L-amino acids. Flow cytometry analysis indicated that both P-I metalloproteinases and LAAO arrested the cells in G0/G1 phase and further induced both necrosis and apoptosis in endothelial cells. In vitro, P-I metalloproteinases and LAAO exhibited significant anti-angiogenic properties in 3D spheroid and Matrigel models by reducing sprout outgrowth and tube formation. Using agarose plug transplants in mice harbouring P-I metalloproteinases and LAAO we demonstrated a marked disruption of vasculature at the periphery. Conclusion: Our research suggests that P-I metalloproteinases and LAAO exhibit anti-angiogenic properties in vitro and in vivo.
DOI: 10.6084/m9.figshare.19904158
title: PUBLIC FUNDING (FIES AND PROUNI) FOR MEDICAL TEACHING IN BRAZIL: A LITERATURE REVIEW AND THE DISTORTIONS CREATED

description: ABSTRACT: Pressure from society, following the expansion of private higher education, resulted in the creation of FIES and PROUNI. With the increase in the number of new medical schools as a result of the More Doctors Program Law, new pressures may occur due to vacant seats, student default, or the reduction of government incentives. The objective of this article is to map the studies on student financing for higher education in Brazil and to conduct a discussion relating the findings to the situation of undergraduate medical education in Brazil. The method used was the Scooping review of the Joanna Briggs Institute with the descriptors: government financing, privatization and academic institutions. 134 articles were found for a final selection of 11 papers. The results showed the importance of government aid for private educational organizations, encouraging the expansion of the segment. For medical education, private expansion is a cause for concern due to the questionable quality of many institutions.
DOI: 10.6084/m9.figshare.19904158.v1
title: PUBLIC FUNDING (FIES AND PROUNI) FOR MEDICAL TEACHING IN BRAZIL: A LITERATURE REVIEW AND THE DISTORTIONS CREATED

description: ABSTRACT: Pressure from society, following the expansion of private higher education, resulted in the creation of FIES and PROUNI. With the increase in the number of new medical schools as a result of the More Doctors Program Law, new pressures may occur due to vacant seats, student default, or the reduction of government incentives. The objective of this article is to map the studies on student financing for higher education in Brazil and to conduct a discussion relating the findings to the situation of undergraduate medical education in Brazil. The method used was the Scooping review of the Joanna Briggs Institute with the descriptors: government financing, privatization and academic institutions. 134 articles were found for a final selection of 11 papers. The results showed the importance of government aid for private educational organizations, encouraging the expansion of the segment. For medical education, private expansion is a cause for concern due to the questionable quality of many institutions.
DOI: 10.6084/m9.figshare.19904159
title: Ecotoxicological assessment of silicate rock fertilizers using soil invertebrates

description: Abstract: The objective of this work was to evaluate the possible adverse effects of rock dusts on the avoidance behavior of earthworms and collembolans, as well as on the survival and reproduction of enchytraeids, in ecotoxicological tests using artificial and natural soils. The tests were performed following international guidelines (ISO 17512-2/2011, ISO 11267/1999, and NBR ISO 16387/2012), with some adaptations, in order to evaluate the effect of dusts from two silicate rocks, biotite schist and phonolite, at the concentrations of 0, 0.1, 1.0, 10, and 100%, in artificial and natural (Arenosol and Ferralsol) soils. The avoidance behavior of the earthworm Eisenia andrei and the collembolan Folsomia candida was evaluated, as well as the survival and reproduction of the enchytraeid Enchytraeus sp. Biotite schist and phonolite affected negatively earthworms when applied to the soil at concentrations equal to or higher than 1.0%. The observed effect was associated to the greater presence of salts, especially in the phonolite, and to changes in soil texture when higher concentrations were used. Negative effects on collembolans and enchytraeids were verified at concentrations of 10% or using pure rock dusts. Agronomic rates smaller than 1.0% (approximately 20 Mg ha-1) rock dusts do not cause avoidance behavior or reduction in the survival and reproduction of invertebrates in the soil.
DOI: 10.6084/m9.figshare.19904159.v1
title: Ecotoxicological assessment of silicate rock fertilizers using soil invertebrates

description: Abstract: The objective of this work was to evaluate the possible adverse effects of rock dusts on the avoidance behavior of earthworms and collembolans, as well as on the survival and reproduction of enchytraeids, in ecotoxicological tests using artificial and natural soils. The tests were performed following international guidelines (ISO 17512-2/2011, ISO 11267/1999, and NBR ISO 16387/2012), with some adaptations, in order to evaluate the effect of dusts from two silicate rocks, biotite schist and phonolite, at the concentrations of 0, 0.1, 1.0, 10, and 100%, in artificial and natural (Arenosol and Ferralsol) soils. The avoidance behavior of the earthworm Eisenia andrei and the collembolan Folsomia candida was evaluated, as well as the survival and reproduction of the enchytraeid Enchytraeus sp. Biotite schist and phonolite affected negatively earthworms when applied to the soil at concentrations equal to or higher than 1.0%. The observed effect was associated to the greater presence of salts, especially in the phonolite, and to changes in soil texture when higher concentrations were used. Negative effects on collembolans and enchytraeids were verified at concentrations of 10% or using pure rock dusts. Agronomic rates smaller than 1.0% (approximately 20 Mg ha-1) rock dusts do not cause avoidance behavior or reduction in the survival and reproduction of invertebrates in the soil.
DOI: 10.6084/m9.figshare.19904161
title: Microalga Scenedesmus obliquus: extraction of bioactive compounds and antioxidant activity

description: ABSTRACT Microalgae have been researched as a sustainable source of bioactive compounds for the food and pharmaceutical industries. The objectives of this work were the extraction and the characterization of fatty acids of Scenedesmus obliquus and to separate, quantify, and evaluate the antioxidant capacity of carotenoids and phenolic compounds of this microalga. The microalga cells were disrupted by using ultrasound. The centesimal composition of lyophilized cells was determined. Fatty acids of the oil were converted into methyl esters and analyzed by gas chromatography (flame ionization detector) to obtain the fatty acid profile. Acetone, petroleum ether, and hexane were the solvents applied to extract the carotenoids and ethanol was used to separate the phenolic compounds. The antioxidant activity of the extracts was determined by the DPPH method. The amount of polyunsaturated fatty acids corresponded to 47.31% of the total for unsaturated, with the predominance of linolenic acid (16.74%) and approximately 9.70% of omega-6. The content of extracted carotenoids was higher by using hexane. Total phenolics concentration of 1.12 g 100 g-1 microalga was found. High percentages of oxidation inhibition were observed for the extracts of carotenoids (&gt;88%) and phenolic compounds (&gt;72%). Therefore, Scenedesmus obliquus presents itself as an alternative, natural, and sustainable source of bioactive compounds.
DOI: 10.6084/m9.figshare.19904161.v1
title: Microalga Scenedesmus obliquus: extraction of bioactive compounds and antioxidant activity

description: ABSTRACT Microalgae have been researched as a sustainable source of bioactive compounds for the food and pharmaceutical industries. The objectives of this work were the extraction and the characterization of fatty acids of Scenedesmus obliquus and to separate, quantify, and evaluate the antioxidant capacity of carotenoids and phenolic compounds of this microalga. The microalga cells were disrupted by using ultrasound. The centesimal composition of lyophilized cells was determined. Fatty acids of the oil were converted into methyl esters and analyzed by gas chromatography (flame ionization detector) to obtain the fatty acid profile. Acetone, petroleum ether, and hexane were the solvents applied to extract the carotenoids and ethanol was used to separate the phenolic compounds. The antioxidant activity of the extracts was determined by the DPPH method. The amount of polyunsaturated fatty acids corresponded to 47.31% of the total for unsaturated, with the predominance of linolenic acid (16.74%) and approximately 9.70% of omega-6. The content of extracted carotenoids was higher by using hexane. Total phenolics concentration of 1.12 g 100 g-1 microalga was found. High percentages of oxidation inhibition were observed for the extracts of carotenoids (&gt;88%) and phenolic compounds (&gt;72%). Therefore, Scenedesmus obliquus presents itself as an alternative, natural, and sustainable source of bioactive compounds.
DOI: 10.6084/m9.figshare.19904162
title: Transcultural validation of an instrument to evaluate Advanced Nursing Practice competences in Brazil

description: ABSTRACT Objectives: to carry out a transcultural validation of the Modified Advanced Practice Nursing Role Delineation Tool for the Brazilian language. Methods: methodological and quantitative study, including the process of translation and cultural adaptation of the instrument. The committee of evaluators was formed by seven experts. Data collection for the pre-test took place from November 2020 to January 2021, with 30 nurses from the West of Paraná. Data analysis used Cronbach’s alpha, intraclass correlation, and kappa. Results: the agreement of the evaluator committee reached a substantial kappa of 0.68 and an intraclass correlation of 0.80, and as a result, the version for application in a pretest was approved. The pretest found a 0.98 Cronbach’s Alpha and an intraclass correlation of 0.61. Conclusions: the instrument was found to be culturally adapted and can be used in the country to identify the competences of the Primary Healthcare Nurses to develop advanced nursing practices.
DOI: 10.6084/m9.figshare.19904162.v1
title: Transcultural validation of an instrument to evaluate Advanced Nursing Practice competences in Brazil

description: ABSTRACT Objectives: to carry out a transcultural validation of the Modified Advanced Practice Nursing Role Delineation Tool for the Brazilian language. Methods: methodological and quantitative study, including the process of translation and cultural adaptation of the instrument. The committee of evaluators was formed by seven experts. Data collection for the pre-test took place from November 2020 to January 2021, with 30 nurses from the West of Paraná. Data analysis used Cronbach’s alpha, intraclass correlation, and kappa. Results: the agreement of the evaluator committee reached a substantial kappa of 0.68 and an intraclass correlation of 0.80, and as a result, the version for application in a pretest was approved. The pretest found a 0.98 Cronbach’s Alpha and an intraclass correlation of 0.61. Conclusions: the instrument was found to be culturally adapted and can be used in the country to identify the competences of the Primary Healthcare Nurses to develop advanced nursing practices.
DOI: 10.6084/m9.figshare.19904164
title: Biological solubilization of phosphate rock by solid-state cultivation to produce eco-friendly fertilizers

description: Abstract: The objective of this work was to evaluate the solubilization of phosphorus from a phosphate rock by Aspergillus niger, under solid-state cultivation (SSC) in sugarcane (Saccharum officinarum) bagasse, by maximizing the efficiency of citric acid production. The phosphate rock (IPR) chosen for the study is a type of igneous rock with a very low phosphorus solubility, obtained from the Itafós company, in Arraias, in the state of Tocantins, Brazil. The rotatable central composite design (RCCD) was used as a statistical tool to evaluate the effect of the concentrations of the carbon source (sucrose) and of the IPR on the SSC medium as a strategy to improve P solubilization. In the process without the IPR, there was a citric acid production of up to 300 g per kilogram of substrate. The experiments in the RCCD showed that the addition of the IPR affected citric acid production, with values of solubilized P ranging from 1.44 to 2.72 g per kilogram of substrate and of solubilized P yield from 12.96 to 48.94%. The analysis of the solubilized P/sucrose ratio showed favorable conditions for P solubilization and citric acid production. The obtained solubilized P values are promising considering that the IPR has a very low solubilization, with only 8.6% P2O5. Overall, these findings could contribute to the development of biotechnological processes for producing eco-friendly phosphate fertilizers, as an alternative for a more sustainable agriculture.
DOI: 10.6084/m9.figshare.19904164.v1
title: Biological solubilization of phosphate rock by solid-state cultivation to produce eco-friendly fertilizers

description: Abstract: The objective of this work was to evaluate the solubilization of phosphorus from a phosphate rock by Aspergillus niger, under solid-state cultivation (SSC) in sugarcane (Saccharum officinarum) bagasse, by maximizing the efficiency of citric acid production. The phosphate rock (IPR) chosen for the study is a type of igneous rock with a very low phosphorus solubility, obtained from the Itafós company, in Arraias, in the state of Tocantins, Brazil. The rotatable central composite design (RCCD) was used as a statistical tool to evaluate the effect of the concentrations of the carbon source (sucrose) and of the IPR on the SSC medium as a strategy to improve P solubilization. In the process without the IPR, there was a citric acid production of up to 300 g per kilogram of substrate. The experiments in the RCCD showed that the addition of the IPR affected citric acid production, with values of solubilized P ranging from 1.44 to 2.72 g per kilogram of substrate and of solubilized P yield from 12.96 to 48.94%. The analysis of the solubilized P/sucrose ratio showed favorable conditions for P solubilization and citric acid production. The obtained solubilized P values are promising considering that the IPR has a very low solubilization, with only 8.6% P2O5. Overall, these findings could contribute to the development of biotechnological processes for producing eco-friendly phosphate fertilizers, as an alternative for a more sustainable agriculture.
DOI: 10.6084/m9.figshare.19904168.v1
title: Impact of the COVID-19 pandemic on emergency department visits: experience of a Brazilian reference center

description: ABSTRACT Objective To analyze the impact of COVID-19 on emergency department metrics at a large tertiary reference hospital in Brazil. Methods A retrospective analysis of consecutive emergency department visits, from January 1, 2020, to November 21, 2020, was performed and compared to the corresponding time frame in 2018 and 2019. The volume of visits and patients’ demographic and clinic characteristics were compared. All medical conditions were included, except confirmed cases of COVID-19. Results A total of 138,138 emergency department visits occurred during the study period, with a statistically significant (p&lt;0.01) reduction by 52% compared to both 2018 and 2019. This decrease was more pronounced for pediatric visits – a drop by 71% in comparison to previous years. Regarding clinical presentation, there was a decrease of severe cases by 34.7% and 37.6%, whereas mild cases decreased by 55.2% and 56.2% when comparing 2020 to 2018 and 2019, respectively. A 30% fall in the total volume of hospital admission from emergency department patients was observed during the study period, but accompanied by a proportional increase in monthly admission rates since April 2020. Conclusion The COVID-19 pandemic led to a 52% fall in attendance at our emergency department for other conditions, along with a proportional increase in hospital admission rates of COVID-19 patients. Healthcare providers should raise patient awareness not to delay seeking medical treatment of severe conditions that require care at the emergency department.
DOI: 10.6084/m9.figshare.19904168
title: Impact of the COVID-19 pandemic on emergency department visits: experience of a Brazilian reference center

description: ABSTRACT Objective To analyze the impact of COVID-19 on emergency department metrics at a large tertiary reference hospital in Brazil. Methods A retrospective analysis of consecutive emergency department visits, from January 1, 2020, to November 21, 2020, was performed and compared to the corresponding time frame in 2018 and 2019. The volume of visits and patients’ demographic and clinic characteristics were compared. All medical conditions were included, except confirmed cases of COVID-19. Results A total of 138,138 emergency department visits occurred during the study period, with a statistically significant (p&lt;0.01) reduction by 52% compared to both 2018 and 2019. This decrease was more pronounced for pediatric visits – a drop by 71% in comparison to previous years. Regarding clinical presentation, there was a decrease of severe cases by 34.7% and 37.6%, whereas mild cases decreased by 55.2% and 56.2% when comparing 2020 to 2018 and 2019, respectively. A 30% fall in the total volume of hospital admission from emergency department patients was observed during the study period, but accompanied by a proportional increase in monthly admission rates since April 2020. Conclusion The COVID-19 pandemic led to a 52% fall in attendance at our emergency department for other conditions, along with a proportional increase in hospital admission rates of COVID-19 patients. Healthcare providers should raise patient awareness not to delay seeking medical treatment of severe conditions that require care at the emergency department.
DOI: 10.15131/shef.data.19509001
title: Fabrication of Infrared Linear Arrays of InAs Planar Avalanche Photodiodes

description: These files correspond to the data presented in: Fabrication of Infrared Linear Arrays of InAs Planar Avalanche Photodiodes
DOI: 10.15131/shef.data.19509001.v1
title: Fabrication of Infrared Linear Arrays of InAs Planar Avalanche Photodiodes

description: These files correspond to the data presented in: Fabrication of Infrared Linear Arrays of InAs Planar Avalanche Photodiodes
DOI: 10.6084/m9.figshare.16484501.v1
title: Platysma transverse myocutaneous flap: a 21 case series of an overlooked reconstructive method for facial skin defects

description: Abstract Introduction Since the first report of a platysma transverse myocutaneous flap in 1977, few articles about this flap design have been added to the literature. Objective Our aim is to describe our department's experience with platysma transverse myocutaneous flap. Methods A retrospective review of all patients undergoing platysma transverse myocutaneous flap reconstruction between 2011 and 2019. Results There were 16 men and 5 women in this series. The mean patients' age was 72.7 years old. In eight cases, we had wound complications, including four wound infections, one hematoma and three distal flap ischemia problems. Distal flap ischemia occurred only in cases that advanced beyond the midline and with length-to-width ratio equal to or over three to one. Neck dissection was performed in two of these three cases with ischemic complications. Conclusion Several factors may influence platysma transverse myocutaneous flap survival. Usually a long and narrow flap, especially crossing the neck midline and associated with neck dissection are more prone to poor outcomes.