cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/mjc09-9nx47
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-01</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-13/rawdata/</p>Test_Pseudomonas_dfliF_20.mrc,  Tilt Series (Pixel Size 0.37 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-13/rawdata/Test_Pseudomonas_dfliF_20.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_20_full.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-13/3dimage_97299/Test_Pseudomonas_dfliF_20_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mkvqb-8sf37
title: MEF cells - Septin2 knock down

description: Septin2 was knockdown using retroviral delivery of shRNA. Purpose - mitochondrial division in the absence of Septin2.
description: <strong>Tilt Series Date:</strong> 2019-01-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun-Shik from Chan lab helped design the shRNA against Septin2 and provided transduction protocol. Shrawan and Danielle Grotjahn created cell line. Danielle FIB-milled the cells and Shrawan performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were treated with 10µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-4/rawdata/</p>TS1.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-4/rawdata/TS1.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS1_bin4_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 997.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-4/3dimage_102021/TS1_bin4_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3ttd4-bb937
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-01</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-25/rawdata/</p>Test_Pseudomonas_dfliF_25_fix.mrc,  Tilt Series (Pixel Size 0.37 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-25/rawdata/Test_Pseudomonas_dfliF_25_fix.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_25_fix_part61_1.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-25/3dimage_97321/Test_Pseudomonas_dfliF_25_fix_part61_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.qkkp5p
title: Occurrence Download

description: A dataset containing 103 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Obelia dichotoma",
 "Country is Mexico",
 "HasCoordinate is true"
 ]
}

 The dataset includes 103 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007311-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/e9rne-twz85
title: HeLa (Kyoto) - PM damaged

description: HeLa cell stably expresses CHMP4B-EGFP at low levels (courtesy MPI). grid - WY41_grid3.
description: <strong>Tilt Series Date:</strong> 2018-05-15</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, caused plasma membrane damage using Laser and froze. Shrawan performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-05-15-1/rawdata/</p>TS2.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-05-15-1/rawdata/TS2.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS2_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-05-15-1/3dimage_102263/TS2_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/33386-1s155
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-77/rawdata/</p>9082_box1_No1_158.mrc,  Tilt Series (Pixel Size 0.2612 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-77/rawdata/9082_box1_No1_158.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_box1_No1_158_full.rec,  Reconstruction (Pixel Size 1.0448 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-77/3dimage_110205/9082_box1_No1_158_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hbm4rt
title: Occurrence Download

description: A dataset containing 18 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Copadichromis diplostigma, Copadichromis geertsi, Copadichromis ilesi, Copadichromis insularis, Copadichromis jacksoni, Copadichromis likomae, Copadichromis mbenjii, Copadichromis melas, Copadichromis mloto, Copadichromis nkatae, Copadichromis parvus, Copadichromis pleurostigma, Copadichromis pleurostigmoides, Copadichromis quadrimaculatus, Copadichromis trewavasae, Copadichromis trimaculatus, Copadichromis verduyni, Copadichromis virginalis, Coptodon bakossiorum, Coptodon bemini, Coptodon bythobates, Coptodon cameronensis, Coptodon camerunensis, Coptodon coffea, Coptodon congica, Coptodon dageti, Coptodon deckerti, Coptodon discolor, Coptodon ejagham, Coptodon flava, Coptodon fusiforme, Coptodon guineensis, Coptodon gutturosa, Coptodon imbriferna, Coptodon ismailiaensis, Coptodon kottae, Coptodon louka, Coptodon margaritacea, Coptodon nigrans, Coptodon nyongana, Coptodon rendalli, Coptodon rheophila, Coptodon snyderae, Coptodon spongotroktis, Coptodon tholloni, Coptodon thysi, Coptodon walteri, Coptodon zillii, Coptostomabarbus bellcrossi, Coptostomabarbus wittei, Corematodus shiranus, Corematodus taeniatus, Coryogalops william, Cotylopus acutipinnis, Cotylopus rubripinnis, Crenimugil buchanani, Crenimugil crenilabis, Crenimugil seheli, Cromeria nilotica, Cromeria occidentalis, Ctenochromis horei, Ctenochromis luluae, Ctenochromis oligacanthus, Ctenochromis polli, Ctenogobius lepturus, Ctenopharyngodon idella, Ctenopharynx intermedius, Ctenopharynx nitidus, Ctenopharynx pictus, Ctenopoma acutirostre, Ctenopoma ashbysmithi, Ctenopoma kingsleyae, Ctenopoma machadoi, Ctenopoma multispine, Ctenopoma maculatum, Ctenopoma muriei, Ctenopoma nebulosum, Ctenopoma nigropannosum, Ctenopoma ocellatum, Ctenopoma petherici, Ctenopoma weeksii, Cunningtonia longiventralis, Cyathochromis obliquidens, Cyathopharynx furcifer, Cyclopharynx fwae, Cyclopharynx schwetzi, Cynotilapia afra, Cynotilapia aurifrons, Cynotilapia axelrodi, Cynotilapia chilundu, Cynotilapia pulpican, Cynotilapia zebroides, Microchromis zebroides, Cyphomyrus discorhynchus, Cyphomyrus macrops, Hippopotamyrus macrops, Cyphomyrus psittacus, Hippopotamyrus psittacus, Cyphomyrus wilverthi, Hippopotamyrus wilverthi)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((46.8009 -14.7158,46.1382 -15.1614,46.1382 -15.9401,50.8081 -15.9401,50.9712 -15.4415,50.3203 -12.5851,49.5255 -11.523,49.0875 -11.4772,48.2794 -11.911,48.1654 -12.5557,47.3945 -13.3102,47.2457 -14.0495,46.8009 -14.7158))"
 ]
}

 The dataset includes 18 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007313-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/3b19x-6yz65
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-01</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-8/rawdata/</p>Test_Pseudomonas_dfliF_16.mrc,  Tilt Series (Pixel Size 0.37 nm), 767.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-8/rawdata/Test_Pseudomonas_dfliF_16.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_16_full.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-8/3dimage_97285/Test_Pseudomonas_dfliF_16_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hyvnvm
title: Occurrence Download

description: A dataset containing 9 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Chrysichthys ogooensis, Chrysichthys okae, Chrysichthys ornatus, Chrysichthys platycephalus, Chrysichthys polli, Chrysichthys punctatus, Chrysichthys sharpii, Chrysichthys stappersii, Chrysichthys teugelsi, Chrysichthys thonneri, Chrysichthys thysi, Chrysichthys turkana, Chrysichthys uniformis, Chrysichthys wagenaari, Chrysichthys walkeri, Cirrhinus cirrhosus, Citharichthys stampflii, Citharidium ansorgii, Citharinops distichodoides, Citharinus citharus, Citharinus congicus, Citharinus eburneensis, Citharinus gibbosus, Citharinus latus, Citharinus macrolepis, Clariallabes attemsi, Clariallabes brevibarbis, Clariallabes centralis, Clariallabes heterocephalus, Clariallabes laticeps, Clariallabes longicauda, Clariallabes manyangae, Clariallabes melas, Clariallabes mutsindoziensis, Clariallabes petricola, Clariallabes platyprosopos, Clariallabes simeonsi, Clariallabes teugelsi, Clariallabes uelensis, Clariallabes variabilis, Clarias agboyiensis, Clarias albopunctatus, Clarias alluaudi, Clarias angolensis, Clarias anguillaris, Clarias buettikoferi, Clarias buthupogon, Clarias camerunensis, Clarias cavernicola, Clarias dhonti, Clarias dumerilii, Clarias ebriensis, Clarias engelseni, Clarias gabonensis, Clarias gariepinus, Clarias hilli, Clarias jaensis, Clarias laeviceps, Clarias lamottei, Clarias liocephalus, Clarias longior, Clarias maclareni, Clarias macromystax, Clarias ngamensis, Clarias nigromarmoratus, Clarias pachynema, Clarias platycephalus, Clarias salae, Clarias stappersii, Clarias submarginatus, Clarias theodorae, Clarias werneri, Clarotes laticeps, Clupeocharax schoutedeni, Clypeobarbus bellcrossi, Clypeobarbus bomokandi, Clypeobarbus congicus, Clypeobarbus hypsolepis, Clypeobarbus matthesi, Clypeobarbus pleuropholis, Clypeobarbus pseudognathodon, Clypeobarbus schoutedeni, Cobitis maroccana, Coelotilapia joka, Congocharax olbrechtsi, Congochromis dimidiatus, Congochromis pugnatus, Congochromis sabinae, Congochromis squamiceps, Congoglanis alula, Congolapia bilineata, Congothrissa gossei, Congresox talabonoides, Copadichromis atripinnis, Copadichromis azureus, Copadichromis borleyi, Copadichromis chizumuluensis, Copadichromis chrysonotus, Copadichromis cyaneus, Copadichromis cyanocephalus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((18.2494 -34.8049,19.1382 -35.0665,19.1382 -33.9401,17.8437 -33.9401,17.8729 -34.3757,18.2494 -34.8049))"
 ]
}

 The dataset includes 9 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007314-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/9azmr-naq73
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-276/rawdata/</p>9082_box2_No3_81.mrc,  Tilt Series (Pixel Size 0.2612 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-276/rawdata/9082_box2_No3_81.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_box2_No3_81_full.rec,  Reconstruction (Pixel Size 1.0448 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-276/3dimage_110615/9082_box2_No3_81_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yfr3r-mtg55
title: Xanthomonas WT

description: Xac WT pBRaφ pBRR
description: <strong>Tilt Series Date:</strong> 2019-12-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Carvalho and Farah lab sent the strain. Debnath Ghosal collected data</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in 2xTY with apt antibiotics, freeze cells next day after about 14 hour when they are in stationary phase.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 5 sec. ** Use 7 sec for next sessions.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-36/rawdata/</p>Xac_No1_TS45.mrc,  Tilt Series (Pixel Size 0.353 nm), 612.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-36/rawdata/Xac_No1_TS45.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_No1_TS45.rec,  Reconstruction (Pixel Size 1.412 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-36/3dimage_112414/Xac_No1_TS45.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nxk4c-wch49
title: MEF cells - Septin2 knock down

description: Septin2 was knockdown using retroviral delivery of shRNA. Purpose - mitochondrial division in the absence of Septin2.
description: <strong>Tilt Series Date:</strong> 2019-01-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun-Shik from Chan lab helped design the shRNA against Septin2 and provided transduction protocol. Shrawan and Danielle Grotjahn created cell line. Danielle FIB-milled the cells and Shrawan performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were treated with 10µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-5/rawdata/</p>TS2.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-5/rawdata/TS2.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS2_bin4_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-5/3dimage_102022/TS2_bin4_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>