DOI: 10.5281/zenodo.1484510
title: Annotation Of Metagenome-Assembled Genomes Retrieved From Amazon River Basin Metagenomes

description: <strong>Annotation of metagenome-assembled genomes retrieved from Amazon river basin metagenomes</strong>

 

                    RELEASE MAG-2018/01<br>
                     --------------------------------------

 

1. INTRODUCTION

Here is deposited the genes and proteins annotation from metagenome-assembled genomes (MAGs) retrieved from Amazon river basin metaganomes (SRP044326, PRJEB25171 and SRP039390) were deposited under European Nucleotide Archive - ENA project PRJEB25176. Briefly, metagenomes were coassembled in groups by geographical location with Megahit v.1.0 and the contigs were used to a reads mapping and binning with BWA-MEM (version 0.7.12-r1039), SamTools (version 1.3.1) and Metabat (v2.12.1). MAGs with overall quality greater than 50, calculated with CheckM (version 1.0.11), were selected for refining precedures. Contigs outliers were eliminated by using RefineM (version 0.0.23). Finished MAGs were then annotated by Prokka (version 1.11) pipeline, and with the other most completes databases up to date (KEGG, UniProtKB, dbCAN, PFAM, eggNOG and COG).

 

2. LOCATION

 

   MAGs sequences are available under ENA project PRJEB25176.

  

    ENA_accession   Isolate<br>
    --------------------    --------------<br>
    ERZ494218    AM_0118<br>
    ERZ494219    AM_0219<br>
    ERZ494220    AM_0226<br>
    ERZ494221    AM_0228<br>
    ERZ494222    AM_0233<br>
    ERZ494223    AM_0240<br>
    ERZ494224    AM_0244<br>
    ERZ494225    AM_0256<br>
    ERZ494226    AM_0268<br>
    ERZ494227    AM_0275<br>
    ERZ494228    AM_0466<br>
    ERZ494229    AM_0507<br>
    ERZ494230    AM_0510<br>
    ERZ494231    AM_0519<br>
    ERZ494232    AM_0528<br>
    ERZ494233    AM_0546<br>
    ERZ494234    AM_0608<br>
    ERZ494235    AM_0615<br>
    ERZ494236    AM_0616<br>
    ERZ494237    AM_0619<br>
    ERZ494238    AM_0621<br>
    ERZ494239    AM_0630<br>
    ERZ494240    AM_0643<br>
    ERZ494241    AM_0729<br>
    ERZ494242    AM_0764<br>
    ERZ494243    AM_0832<br>
    ERZ494244    AM_0849<br>
    ERZ494245    AM_0854<br>
    ERZ494246    AM_0876<br>
    ERZ494247    AM_0902<br>
    ERZ494248    AM_0936<br>
    ERZ494249    AM_1003<br>
    ERZ494250    AM_1104<br>
    ERZ494251    AM_1111<br>
    ERZ494252    AM_1205<br>
    ERZ494253    AM_1312<br>
    ERZ494254    AM_1409<br>
    ERZ494255    AM_1503<br>
    ERZ494256    AM_1603<br>
    ERZ494257    AM_1606<br>
    ERZ494258    AM_1801<br>
    ERZ494259    AM_1811<br>
    ERZ494260    AM_2104<br>
    ERZ494261    AM_2116<br>
    ERZ494262    AM_2124<br>
    ERZ494263    AM_2202<br>
    ERZ494264    AM_2207<br>
    ERZ494265    AM_2208<br>
    ERZ494266    AM_2324<br>
    ERZ494267    AM_2502<br>
    ERZ494268    AM_2804<br>
   

3. ACKNOWLEDGEMENTS<br>
   

This work is a joint effort of Laboratory of molecular biology from Federal<br>
University of São Carlos, São Paulo, Brazil (LBM/UFSCAR) and Protists group<br>
of Institut del Ciencias del Mar, Barcelone, Spain (ICM). We are grateful to<br>
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), as well as,                     the spanish funding organ Consejo Superior de Investigaciones Científicas (CSIC).

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

 

4. CONTACT INFORMATION

   Current curators:

   - Célio Dias Santos Júnior (celio.diasjunior@gmail.com)<br>
   - Flavio Henrique-Silva (dfhs@ufscar.br)<br>
   - Ramiro R. Logares (ramiro.logares@icm.csic.es)<br>
 

5. COPYRIGHT NOTICE

   Amazon River Basin Metagenome-Assembled Genomes Annotation - AM/MAGs<br>
   Copyright (C) 2018 The AMnrGC consortium.

   This database is provided “as is” and without any warranty of any kind,<br>
   of openly available. You can redistribute and/or modify it<br>
   as you wish, under the terms of Creative Commons CC BY 4.0:

    https://creativecommons.org/licenses/by/4.0/

___________________<br>
Barcelone, Feb/2018