DOI: 10.5281/zenodo.14954496
title: Nickel Experiment Data from Enriched Nitrifying Sludge

description: Raw data for Ni 1 mg/L experiment.




ONTOLOGY SOURCE REFERENCE
 
 


Term Source Name
ENVO
ENVO


Term Source File
http://purl.obolibrary.org/obo/ENVO_00002046
http://purl.obolibrary.org/obo/ENVO_00002046


Term Source Description
The Environment Onthology
The Environment Onthology


STUDY
 
 


Study Identifier
Ni 1 mg/L
Ni 1 mg/L


Study Title
Nickel impact experiments
Nickel impact experiments


Study Description
Determination of nitrification kinetics, microbial abundance, and functional characteristics after Ni dosage.
Determination of nitrification kinetics, microbial abundance, and ffunctional characteristics after Ni dosage.


Study Submission Date
1.03.2025
1.03.2025


STUDY DESIGN DESCRIPTORS
 
 


Study Design Type
aerobic bioreactor
aerobic bioreactor


Study Design Type Term Accession Number
http://purl.obolibrary.org/obo/ENVO_00002126
http://purl.obolibrary.org/obo/ENVO_00002126


Study Design Type Term Source REF
ENVO
ENVO


STUDY FACTORS
 
 


Study Factor Name
oxygen
substrate


Study Factor Type
chemical compound
chemical compound


Study Factor Type Term Accession Number
http://purl.obolibrary.org/obo/OMIT_0011105
http://purl.obolibrary.org/obo/OMIT_0002057


Study Factor Type Term Source REF
OMIT
OMIT


STUDY ASSAYS
 
 


Study Assay File Name
a_Ni_1_OUR.txt
a_Ni_1_Nitrogencompounds.txt


Study Assay Measurement Type
aerobic respiration, using ammonia as electron donor, aerobic respiration, using nitrite as electron donoraerobic respiration
concentration of nitrate and nitrite in liquid water


Study Assay Measurement Type Term Accession Number
http://purl.obolibrary.org/obo/GO_0019409, http://purl.obolibrary.org/obo/GO_0019332
http://purl.obolibrary.org/obo/ENVO_03000123


Study Assay Measurement Type Term Source REF
ENVO
ENVO


Study Assay Technology Type
respirometry
NH4-N, NO2-N, NO3-N measurement


STUDY PROTOCOLS
 
 


Study Protocol Name
respirometry
ammonia nitrogen determination, nitrite nitrogen determination, nitrate nitrogen determination


Study Protocol Description
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Ni, macro and micro-nutrients. Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software was used for dissolved oxygen measurements and OUR was calculated.
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Ni, macro and micro-nutrients. Nitrogen measurements were conducted in Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software. Samples were filtered through 0,22Um filters.


Study Protocol Parameters Name
oxygen uptake rate
NH4-N, NO2-N, NO3-N




 

Raw data for Ni 2 mg/L experiment.




ONTOLOGY SOURCE REFERENCE
 
 


Term Source Name
ENVO
ENVO


Term Source File
http://purl.obolibrary.org/obo/ENVO_00002046
http://purl.obolibrary.org/obo/ENVO_00002046


Term Source Description
The Environment Onthology
The Environment Onthology


STUDY
 
 


Study Identifier
Ni 2 mg/L
Ni 2 mg/L


Study Title
Nickel impact experiments
Nickel impact experiments


Study Description
Determination of nitrification kinetics, microbial abundance, functional characteristics after Ni dosage.
Determination of nitrification kinetics, microbial abundance, functional characteristics after Ni dosage.


Study Submission Date
1.03.2025
1.03.2025


STUDY DESIGN DESCRIPTORS
 
 


Study Design Type
aerobic bioreactor
aerobic bioreactor


Study Design Type Term Accession Number
http://purl.obolibrary.org/obo/ENVO_00002126
http://purl.obolibrary.org/obo/ENVO_00002126


Study Design Type Term Source REF
ENVO
ENVO


STUDY FACTORS
 
 


Study Factor Name
oxygen
substrate


Study Factor Type
chemical compound
chemical compound


Study Factor Type Term Accession Number
http://purl.obolibrary.org/obo/OMIT_0011105
http://purl.obolibrary.org/obo/OMIT_0002057


Study Factor Type Term Source REF
OMIT
OMIT


STUDY ASSAYS
 
 


Study Assay File Name
a_Ni_2_OUR.txt
a_Ni_2_Nitrogencompounds.txt


Study Assay Measurement Type
aerobic respiration, using ammonia as electron donor, aerobic respiration, using nitrite as electron donoraerobic respiration
concentration of nitrate and nitrite in liquid water


Study Assay Measurement Type Term Accession Number
http://purl.obolibrary.org/obo/GO_0019409, http://purl.obolibrary.org/obo/GO_0019332
http://purl.obolibrary.org/obo/ENVO_03000123


Study Assay Measurement Type Term Source REF
ENVO
ENVO


Study Assay Technology Type
respirometry
NH4-N, NO2-N, NO3-N measurement


STUDY PROTOCOLS
 
 


Study Protocol Name
respirometry
ammonia nitrogen determination, nitrite nitrogen determination, nitrate nitrogen determination


Study Protocol Description
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Ni, macro and micro-nutrients. Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software was used for dissolved oxygen measurements and OUR was calculated.
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Ni, macro and micro-nutrients. Nitrogen measurements were conducted in Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software. Samples were filtered through 0,22Um filters.


Study Protocol Parameters Name
oxygen uptake rate
NH4-N, NO2-N, NO3-N




 

Raw data for Ni 10 mg/L experiment.




ONTOLOGY SOURCE REFERENCE
 
 


Term Source Name
ENVO
ENVO


Term Source File
http://purl.obolibrary.org/obo/ENVO_00002046
http://purl.obolibrary.org/obo/ENVO_00002046


Term Source Description
The Environment Onthology
The Environment Onthology


STUDY
 
 


Study Identifier
Ni 10 mg/L
Ni 10 mg/L


Study Title
Nickel impact experiments
Nickel impact experiments


Study Description
Determination of nitrification kinetics, microbial abundance, functional characteristics after Ni dosage.
Determination of nitrification kinetics, microbial abundance, functional characteristics after Ni dosage.


Study Submission Date
1.03.2025
1.03.2025


STUDY DESIGN DESCRIPTORS
 
 


Study Design Type
aerobic bioreactor
aerobic bioreactor


Study Design Type Term Accession Number
http://purl.obolibrary.org/obo/ENVO_00002126
http://purl.obolibrary.org/obo/ENVO_00002126


Study Design Type Term Source REF
ENVO
ENVO


STUDY FACTORS
 
 


Study Factor Name
oxygen
substrate


Study Factor Type
chemical compound
chemical compound


Study Factor Type Term Accession Number
http://purl.obolibrary.org/obo/OMIT_0011105
http://purl.obolibrary.org/obo/OMIT_0002057


Study Factor Type Term Source REF
OMIT
OMIT


STUDY ASSAYS
 
 


Study Assay File Name
a_Ni_10_OUR.txt
a_Ni_10_Nitrogencompounds.txt


Study Assay Measurement Type
aerobic respiration, using ammonia as electron donor, aerobic respiration, using nitrite as electron donoraerobic respiration
concentration of nitrate and nitrite in liquid water


Study Assay Measurement Type Term Accession Number
http://purl.obolibrary.org/obo/GO_0019409, http://purl.obolibrary.org/obo/GO_0019332
http://purl.obolibrary.org/obo/ENVO_03000123


Study Assay Measurement Type Term Source REF
ENVO
ENVO


Study Assay Technology Type
respirometry
NH4-N, NO2-N, NO3-N measurement


STUDY PROTOCOLS
 
 


Study Protocol Name
respirometry
ammonia nitrogen determination, nitrite nitrogen determination, nitrate nitrogen determination


Study Protocol Description
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Ni, macro and micro-nutrients. Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software was used for dissolved oxygen measurements and OUR was calculated.
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Ni, macro and micro-nutrients. Nitrogen measurements were conducted in Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software. Samples were filtered through 0,22Um filters.


Study Protocol Parameters Name
oxygen uptake rate
NH4-N, NO2-N, NO3-N