DOI: 10.4231/94pz-ab06
title: 3D Image Data from In-situ X-ray Imaging Transverse Crack Growth Experiments in Human Cortical Bone

description: &lt;p&gt;This publication documents 3D image stacks from HR-pQCT imaging of a femur diaphysis, as well as image stacks for two in-situ loaded fracture mechanics specimens observed with 3D X-ray microscopy.&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Imaging&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;For HR-qQCT:&lt;/p&gt;

&lt;p&gt;HR-pQCT scans were acquired by Rachel Surowiec using an XtremeCT II scanner (SCANCO Medical AG, Bruttisellen, Switzerland) within the Musculoskeletal Function, Imaging and Tissue (MSK-FIT) Resource Core of the Indiana Center for Musculoskeletal Health&rsquo;s Clinical Research Center (Indiana University, Indianapolis, IN). &lt;span data-ccp-charstyle="normaltextrun" data-ccp-charstyle-defn="{&quot;ObjectId&quot;:&quot;e4109cb5-34d2-42a9-80dc-3133b20bfe8b|135&quot;,&quot;ClassId&quot;:1073872969,&quot;Properties&quot;:[469775450,&quot;normaltextrun&quot;,201340122,&quot;1&quot;,134233614,&quot;true&quot;,469778129,&quot;normaltextrun&quot;,335572020,&quot;1&quot;,469778324,&quot;Default Paragraph Font&quot;]}"&gt;Scans are performed at 60.7 um resolution, a 68 kV, 1467 &lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt;uA&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt;, 43 &lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt;ms&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt; integration time, 1 frame averaging.&lt;/span&gt; Raw scans are &lt;span data-ccp-charstyle="normaltextrun"&gt;&lsquo;.RSQ&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt;&rsquo; file types&lt;/span&gt;. The ISQ file type were read into ImageJ using the Import&lt;span data-ccp-charstyle="normaltextrun"&gt;-KHKs&lt;/span&gt; &lt;span data-ccp-charstyle="normaltextrun"&gt;Scanco&lt;/span&gt; &lt;span data-ccp-charstyle="normaltextrun"&gt;uCT&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt; ISQ file reader&lt;/span&gt; plug-in, and exported as bmp image stacks, image stacks are provided in two parts. Reconstructed images are rotated in &lt;span data-ccp-charstyle="normaltextrun"&gt;dataviewer&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt; so that all bones are in the same orientation (&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt;prox&lt;/span&gt;&lt;span data-ccp-charstyle="normaltextrun"&gt;/distal/anterior/posterior for the Femur).&lt;/span&gt;&lt;/p&gt;

&lt;p&gt;For in-situ fracture mechanics experiments:&lt;/p&gt;

&lt;p&gt;3D scans were acquired by Glynn Gallaway using a 3-point bending rig for single edged notched bend specimens with a Deben CT5000N load cell (Deben, Bury St. Edmunds, UK) in a Zeiss XRADIA 510 Versa 3D X-Ray microscope (Carl Zeiss AG, Baden-W&uuml;rttemberg, Germany) at Purdue University. The 3-point bending frame had a span 20 mm with X-ray transparent, glassy carbon supports. To maintain hydration, the beam was wrapped in a plastic film slit at the notch. Displacements were applied at 0.1 mm/min. Load cell outputs were monitored and recorded. Displacements are held constant during image acquisitions. The first 3D image was obtained at the onset of non-linearity. Subsequently, the displacement was increased until a load increase of 10 N was observed, and another image was obtained. This sequence was repeated 6-times until peak load. 3D X-ray images were acquired with a resolution of 4.5 um, exposure time 5 sec., 801 projections, 120 kV, 10 W, 4 x objective, and a LE2 filter. X-ray projections were processed through XRADIA Scout-and-Scan Reconstructor. A recursive Gaussian smoothing filter (s=1 pixel) was applied to reduce image artifacts. Image stacks are exported as tiff files and provided individually for each load step and specimen. Two experiments are documented (beam 1 and beam 2).&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Materials&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;The diaphysis of a human (92-year-old, male) cadaveric femur was obtained through the Indiana University School of Medicine Anatomical Donation Program.&lt;/p&gt;
description: This publication documents 3D image stacks in support of the publication "Quasi-brittle Fracture Mechanics of Human Cortical Bone Enables the Combined Analysis of Bone Quantity and Quality" G. Gallaway et al, Journal of Biomechanics.