DOI: 10.5061/dryad.s4mw6m99s
title: Data from: Anatomy of an agricultural antagonist: Feeding complex structure and function of three xylem sap-feeding insects illuminated with synchrotron-based 3D imaging

description: Many insects feed on xylem or phloem sap of vascular plants. Although
 physical damage to the plant is minimal, the process of insect feeding can
 inadvertently transmit lethal viruses and bacterial pathogens. 
 Disparities between insect-mediated pathogen transmission efficiency have
 been identified among xylem sap-feeding insects; however, the mechanistic
 drivers of these trends are unclear. Identifying and understanding the
 structural factors and associated integrated functional components that
 may ultimately determine these disparities is critical for managing plant
 diseases. Here, we applied synchrotron-based X-ray microcomputed
 tomography to digitally reconstruct the morphology of three xylem
 sap-feeding insect vectors of plant pathogens: Graphocephala atropunctata
 (blue-green sharpshooter) and Homalodisca vitripennis (glassy-winged
 sharpshooter) (Hemiptera, Cicadellidae), and the spittlebug Philaenus
 spumarius (meadow spittlebug, Hemiptera, Aphrophoridae). The application
 of this technique revealed previously undescribed anatomical features of
 these organisms, such as key components of the salivary complex, and
 visualization of the 3D structure of the precibarial valve led to new
 insights into the mechanism of how this structure functions. Morphological
 disparities with functional implications between taxa were highlighted as
 well, including the morphology and volume of the cibarial dilator
 musculature responsible for extracting xylem sap, which has implications
 for force application capabilities. These morphological insights will be
 used to target analyses illuminating functional differences in feeding
 behavior.
description: This dataset includes 3D imaging files of specimens of the
 blue-green sharpshooter (<em>Graphocephala atropunctata</em>),
 the glassy-winged sharpshooter (<em>Homalodisca
 vitripennis</em>), and the meadow spittlebug (<em>Philaenus
 spumarius</em>), including data from synchrotron-based micro-CT
 scans (TIFF stacks), 3D object files (.obj), and Maya files
 (.mb).
description: TIFF stacks: Image file visualization software (e.g., Preview,
 ImageJ) or 3D image processing software (e.g., Dragonfly, Avizo, VG Studio
 MAX). Pixel size of 0.001605mm. .obj files: 3D object
 visualization software (e.g., Meshlab, Autodesk Maya)
 .mb files: Autodesk Maya
description: #Data from: Anatomy of an agricultural antagonist: Feeding complex
 structure and function of three xylem sap-feeding insects illuminated with
 synchrotron-based 3D imaging This is the Dryad repository for "The
 feeding complex of three xylem sap-feeding insects visualized using
 synchrotron-based micro-CT imaging" by Clark et al. This dataset
 includes data from synchrotron-based micro-CT scans (TIFF stacks), 3D
 object files (.obj), and Maya files (.mb) from specimens of the blue-green
 sharpshooter (*Graphocephala atropunctata*), the glassy-winged
 sharpshooter (*Homalodisca* *vitripennis*) and the meadow spittlebug
 (*Philaenus* *spumarius*). TIFF stacks can be opened using image file
 visualization software (e.g., Preview, ImageJ) or 3D image processing
 software (e.g., Dragonfly, Avizo, VG Studio MAX). Images have a pixel size
 of 0.001605mm. .obj files can be opened using 3D object visualization
 software (e.g., Meshlab). Meshlab is an opensource software available at
 https://www.meshlab.net/ .mb files can be opened using Autodesk Maya.
 Autodesk Maya is available with a student and educators license at
 https://www.autodesk.com/education/edu-software/overview