cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5734509.v1
title: Pain during tracheal aspiration in patients with traumatic brain injury undergoing mechanical ventilation

description: ABSTRACT BACKGROUND AND OBJECTIVES: Victims of traumatic brain injury, in intensive care units, frequently experience pain. Tracheal aspiration is a procedure with nociceptive potential routinely carried out in these patients. The objective of this study was to evaluate the effectiveness of tracheal aspiration in patients with traumatic brain injury undergoing mechanical ventilation. METHODS: Prospective study conducted in two intensive care units of a general public hospital in Aracaju, Sergipe, Brazil. During three days, 300 observations were carried out in 20 victims of traumatic brain injury. The pain was assessed using the Brazilian version of the Behavioral Pain Scale and the physiological parameters of heart rate and blood pressure (systolic and diastolic). The sedation depth was measured by Ramsay scores and the Richmond Agitation Sedation Scale. The Friedman test, ANOVA, and the Bonferroni post hoc test were used to verify the existence any differences in pain scores and physiological parameters at the different moments of the evaluation. A 5% statistical significance was accepted. RESULTS: The sample was predominantly comprised of men, young, from the interior of the State, with no comorbidities and with severe traumatic brain injury. Fentanyl and midazolam were the most used drugs for sedation and analgesia. There was a high prevalence of pain (70.0-85.5%). The pain scores were significantly higher during the tracheal aspiration, and the physiological parameters did not present any statistically significant increase. CONCLUSION: Valid and trustworthy behavioral scales, as the Behavioral Pain Scale, should be incorporated into the routine of the intensive care units to guide analgesia and sedation management, especially to prevent suffering during these painful procedures.
DOI: 10.6084/m9.figshare.5734506
title: Follicle Viability after Vitrification of Bovine Ovarian Tissue

description: Abstract Purpose The present study aimed to evaluate the impact of vitrification on the viability of follicles using a three-dimensional (3D) in vitro culture. Methods Bovine ovarian tissue samples (n = 5) obtained from slaughterhouses were utilized. The cortex was cut into small fragments of 2 x 3 x 0.5 mm using a tissue slicer. From these fragments, secondary follicles were first isolated by mechanical and enzymatic methods, then encapsulated in alginate gel and individually cultured for 20 days. Additional fragments of the same ovarian tissue were vitrified in a solution containing 25% glycerol and 25% ethylene glycol. After warming, the follicles underwent the same follicular isolation process that was performed for the fresh follicles. Results A total of 61 follicles were isolated, 51 from fresh ovarian tissue, and 10 from vitrified tissue. After the culture, the vitrified and fresh follicles showed 20% and 43.1% survival rates respectively (p = 0.290),with no significant differences. At the end of the culture, therewere no significant differences in follicular diameter between the vitrified (422.93 ± 85.05 μm) and fresh (412.99 ± 102.55 μm) groups (p = 0.725). Fresh follicles showed higher mean rate of antrum formation when compared with vitrified follicles (47.1% and 20.0% respectively), but without significant difference (p = 0.167). Conclusions The follicles were able to develop, grow and form antrum in the 3D system after vitrification, despite the lower results obtained with the fresh tissue.
DOI: 10.6084/m9.figshare.5734506.v1
title: Follicle Viability after Vitrification of Bovine Ovarian Tissue

description: Abstract Purpose The present study aimed to evaluate the impact of vitrification on the viability of follicles using a three-dimensional (3D) in vitro culture. Methods Bovine ovarian tissue samples (n = 5) obtained from slaughterhouses were utilized. The cortex was cut into small fragments of 2 x 3 x 0.5 mm using a tissue slicer. From these fragments, secondary follicles were first isolated by mechanical and enzymatic methods, then encapsulated in alginate gel and individually cultured for 20 days. Additional fragments of the same ovarian tissue were vitrified in a solution containing 25% glycerol and 25% ethylene glycol. After warming, the follicles underwent the same follicular isolation process that was performed for the fresh follicles. Results A total of 61 follicles were isolated, 51 from fresh ovarian tissue, and 10 from vitrified tissue. After the culture, the vitrified and fresh follicles showed 20% and 43.1% survival rates respectively (p = 0.290),with no significant differences. At the end of the culture, therewere no significant differences in follicular diameter between the vitrified (422.93 ± 85.05 μm) and fresh (412.99 ± 102.55 μm) groups (p = 0.725). Fresh follicles showed higher mean rate of antrum formation when compared with vitrified follicles (47.1% and 20.0% respectively), but without significant difference (p = 0.167). Conclusions The follicles were able to develop, grow and form antrum in the 3D system after vitrification, despite the lower results obtained with the fresh tissue.
DOI: 10.6084/m9.figshare.5734503
title: FAST SIMULTANEOUS DETERMINATION OF PROPIFENAZONE, PARACETAMOL AND CAFFEINE USING BATCH INJECTION ANALYSIS WITH AMPEROMETRIC DETECTION

description: A fast and simple procedure for simultaneous determination of propyphenazone (PRO), paracetamol (PAR) and caffeine (CAF) was performed using batch injection analysis with multiple pulse amperometric detection (BIA-MPA). Three potentials pulses were selected for the analysis: +0.90 V/50 ms: only PAR was oxidized; +1.20 V/50 ms: oxidation of PAR and PRO without the interference of CAF; +1.60 V/50 ms: oxidation of the three compounds (PAR, PRO and CAF). The sample aliquot (150 µL) was directly injected onto the boron-doped diamond (BDD) electrode immersed in a BIA cell. The analytical characteristics of the proposed method include high analytical frequency (75 injections per hour), good stability (RSD &lt; 3.9%; n = 10), and low detection limits (0.3, 0.2 and 0.1 mg L−1 for PRO, PAR and CAF, respectively). The proposed method yielded similar results to those obtained by liquid chromatography at a 95% confidence level.
DOI: 10.6084/m9.figshare.5734497
title: ENVIRONMENTALLY FRIENDLY QUANTUM DOTS: HIGHLIGHTING ZINC OXIDE

description: Semiconductor nanocrystals with sizes in the quantum confinement regime (2-10 nm) present special physical and chemical properties. Additionally, environment-friendly quantum dots (QDs), as zinc oxide and zinc sulfide, offer many practical usages. Herein the ZnO semiconductor nanocrystals properties will be preferentially explored, such as its luminescence, broad spectrum UV absorber and electronics performances, and therefore its multifunctionality, as well as its advantages compared to some toxics QDs. A review is carefully presented stressing some synthesis approaches for applications reasons toward devices, chemosensors, biological labels, UV-absorbers and photocatalysis. ZnO QDs have been used in combination with organic and other inorganic materials. Hybrid materials have many advantages compared to their individual contents leading to important contributions to science and technology. As a result, an important growth in material fields is noticeable.
DOI: 10.6084/m9.figshare.5734497.v1
title: ENVIRONMENTALLY FRIENDLY QUANTUM DOTS: HIGHLIGHTING ZINC OXIDE

description: Semiconductor nanocrystals with sizes in the quantum confinement regime (2-10 nm) present special physical and chemical properties. Additionally, environment-friendly quantum dots (QDs), as zinc oxide and zinc sulfide, offer many practical usages. Herein the ZnO semiconductor nanocrystals properties will be preferentially explored, such as its luminescence, broad spectrum UV absorber and electronics performances, and therefore its multifunctionality, as well as its advantages compared to some toxics QDs. A review is carefully presented stressing some synthesis approaches for applications reasons toward devices, chemosensors, biological labels, UV-absorbers and photocatalysis. ZnO QDs have been used in combination with organic and other inorganic materials. Hybrid materials have many advantages compared to their individual contents leading to important contributions to science and technology. As a result, an important growth in material fields is noticeable.
DOI: 10.6084/m9.figshare.5734494
title: Carbohydrate mouth rinse reduces rating of perceived exertion but does not affect performance and energy systems contribution during a high-intensity exercise

description: ABSTRACT Aim The study aimed to verify the effect of carbohydrate (CHO) mouth rinse on time to exhaustion, energy systems contribution and rating of perceived exertion (RPE) during a high-intensity exercise. Methods Fourteen men performed an incremental exercise test to determine their maximal oxygen uptake and peak power output (PPO) and two time-to-exhaustion tests at 110% of PPO. Participants rinsed their mouth with 25ml of 6.4% of CHO or placebo (PLA) solution immediately before the time-to-exhaustion test, using a crossover design. The contribution of the energy systems was calculated using the free software GEDAE-LaB®. Results Time to exhaustion was similar between the conditions (CHO:174.3±42.8s; PLA:166.7±26.3s; p=0.33). In addition, there was no difference between the CHO and PLA condition for aerobic (CHO:135.1±41.2kJ and PLA:129.8±35.3kJ, p=0.34), anaerobic lactic (CHO:57.6±17.1kJ and PLA:53.4±15.1kJ, p=0.10), and anaerobic alactic (CHO:10.4±8.4kJ and PLA:13.2±9.2kJ, p=0.37) contribution. Consequently, total energy expenditure was similar between conditions (CHO:203.2±46.4kJ and PLA:196.5±45.2kJ, p=0.15). However, CHO mouth rinse reduced the RPE at the moment of exhaustion (CHO:18.2±1.0units and PLA:19.1±1.1units; p=0.02). Conclusion CHO mouth rinse neither increased time to exhaustion nor altered energy systems contribution during a high-intensity exercise, but reduced the perceived effort at the exhaustion.
DOI: 10.6084/m9.figshare.5734494.v1
title: Carbohydrate mouth rinse reduces rating of perceived exertion but does not affect performance and energy systems contribution during a high-intensity exercise

description: ABSTRACT Aim The study aimed to verify the effect of carbohydrate (CHO) mouth rinse on time to exhaustion, energy systems contribution and rating of perceived exertion (RPE) during a high-intensity exercise. Methods Fourteen men performed an incremental exercise test to determine their maximal oxygen uptake and peak power output (PPO) and two time-to-exhaustion tests at 110% of PPO. Participants rinsed their mouth with 25ml of 6.4% of CHO or placebo (PLA) solution immediately before the time-to-exhaustion test, using a crossover design. The contribution of the energy systems was calculated using the free software GEDAE-LaB®. Results Time to exhaustion was similar between the conditions (CHO:174.3±42.8s; PLA:166.7±26.3s; p=0.33). In addition, there was no difference between the CHO and PLA condition for aerobic (CHO:135.1±41.2kJ and PLA:129.8±35.3kJ, p=0.34), anaerobic lactic (CHO:57.6±17.1kJ and PLA:53.4±15.1kJ, p=0.10), and anaerobic alactic (CHO:10.4±8.4kJ and PLA:13.2±9.2kJ, p=0.37) contribution. Consequently, total energy expenditure was similar between conditions (CHO:203.2±46.4kJ and PLA:196.5±45.2kJ, p=0.15). However, CHO mouth rinse reduced the RPE at the moment of exhaustion (CHO:18.2±1.0units and PLA:19.1±1.1units; p=0.02). Conclusion CHO mouth rinse neither increased time to exhaustion nor altered energy systems contribution during a high-intensity exercise, but reduced the perceived effort at the exhaustion.
DOI: 10.6084/m9.figshare.5734491
title: NEW SEMISYNTHETIC DERIVATIVES OF A BENZYLISOTHIOCYANATE ISOLATED FROM Moringa oleifera AND EVALUATION OF THEIR CYTOTOXIC ACTIVITY

description: From the natural product 4-(4'-O-acetyl-α-L-rhamnosyloxy)benzylisothiocyanate (1), isolated from the flowers of Moringa oleifera Lam (Moringaceae), four new semisynthetic derivatives, N-[4-(4'-O-acetyl-α-L-rhamnosyloxy)benzyl]-2-(pyridinil-4-carbonil)hydrazine-1-carbothioamide (3), 4-(4'-O-acetyl-2',3'-dimesyloxy-α-L-rhamnosyloxy)benzylisothiocyanate (4), N-[(4'-O-acetyl-α-L-rhamnosyloxy)benzyl]hydrazinecarbothioamide (5), 4-[4'-O-acetyl-2',3'-O-bis(decanoiloxy)-α-L-rhamnosyloxy]benzylisothiocianate (6), and the known compound 4-(2',3',4'-O-triacetyl-α-L-rhamnosyloxy)benzylisothiocyanate (2), were obtained. All compounds were tested for their cytotoxicity against the tumor cell lines SF-295, HL-60, HCT-116 e PC-3. The natural product 1 and the semisynthetic derivatives 2 and 4 were the most active compounds (IC50 from16.0 to 3.7 µmol L-1) against all tumor cell lines.
DOI: 10.6084/m9.figshare.5734491.v1
title: NEW SEMISYNTHETIC DERIVATIVES OF A BENZYLISOTHIOCYANATE ISOLATED FROM Moringa oleifera AND EVALUATION OF THEIR CYTOTOXIC ACTIVITY

description: From the natural product 4-(4'-O-acetyl-α-L-rhamnosyloxy)benzylisothiocyanate (1), isolated from the flowers of Moringa oleifera Lam (Moringaceae), four new semisynthetic derivatives, N-[4-(4'-O-acetyl-α-L-rhamnosyloxy)benzyl]-2-(pyridinil-4-carbonil)hydrazine-1-carbothioamide (3), 4-(4'-O-acetyl-2',3'-dimesyloxy-α-L-rhamnosyloxy)benzylisothiocyanate (4), N-[(4'-O-acetyl-α-L-rhamnosyloxy)benzyl]hydrazinecarbothioamide (5), 4-[4'-O-acetyl-2',3'-O-bis(decanoiloxy)-α-L-rhamnosyloxy]benzylisothiocianate (6), and the known compound 4-(2',3',4'-O-triacetyl-α-L-rhamnosyloxy)benzylisothiocyanate (2), were obtained. All compounds were tested for their cytotoxicity against the tumor cell lines SF-295, HL-60, HCT-116 e PC-3. The natural product 1 and the semisynthetic derivatives 2 and 4 were the most active compounds (IC50 from16.0 to 3.7 µmol L-1) against all tumor cell lines.
DOI: 10.6084/m9.figshare.5734485
title: Stress State at the Vertex of a Composite Wedge, One Side of Which Slides Without Friction Along a Rigid Surface

description: Abstract For studying the stress-strain state at singular points and their neighborhoods new concept is proposed. A singular point is identified with an elementary volume that has a characteristic size of the real body representative volume. This makes it possible to set and study the restrictions at that point. It is shown that problems with singular points turn out to be ambiguous, their formulation depends on the combination of the material and geometric parameters of the investigated body. Number of constraints in a singular point is redundant compared to the usual point of the boundary (it makes singular point unique, exclusive). This circumstance determines the non-classical problem formulation for bodies containing singular points. The formulation of a non-classical problem is given, the uniqueness of its solution is proved (under the condition of existence), the algorithm of the iterative-analytical decision method is described. Restrictions on the state parameters at the composite wedge vertex, one generatrix of which is in non-friction contact with a rigid surface are studied under temperature and strength loading. The proposed approach allows to identify critical combinations of material and geometric parameters that define the singularity of stress and strain fields close to singular representative volumes. The constraints on load components needed to solution existence are established. An example of a numerical analysis of the state parameters at the wedge vertex and its neighborhood is considered. Solutions built on the basis of a new concept, directly in a singular point, and its small neighborhood differ significantly from the solutions made with asymptotic methods. Beyond a small neighborhood of a singular point the solutions obtained on the basis of different concepts coincide.
DOI: 10.6084/m9.figshare.5734485.v1
title: Stress State at the Vertex of a Composite Wedge, One Side of Which Slides Without Friction Along a Rigid Surface

description: Abstract For studying the stress-strain state at singular points and their neighborhoods new concept is proposed. A singular point is identified with an elementary volume that has a characteristic size of the real body representative volume. This makes it possible to set and study the restrictions at that point. It is shown that problems with singular points turn out to be ambiguous, their formulation depends on the combination of the material and geometric parameters of the investigated body. Number of constraints in a singular point is redundant compared to the usual point of the boundary (it makes singular point unique, exclusive). This circumstance determines the non-classical problem formulation for bodies containing singular points. The formulation of a non-classical problem is given, the uniqueness of its solution is proved (under the condition of existence), the algorithm of the iterative-analytical decision method is described. Restrictions on the state parameters at the composite wedge vertex, one generatrix of which is in non-friction contact with a rigid surface are studied under temperature and strength loading. The proposed approach allows to identify critical combinations of material and geometric parameters that define the singularity of stress and strain fields close to singular representative volumes. The constraints on load components needed to solution existence are established. An example of a numerical analysis of the state parameters at the wedge vertex and its neighborhood is considered. Solutions built on the basis of a new concept, directly in a singular point, and its small neighborhood differ significantly from the solutions made with asymptotic methods. Beyond a small neighborhood of a singular point the solutions obtained on the basis of different concepts coincide.
DOI: 10.6084/m9.figshare.5734470
title: Validation of anthropometric models in the estimation of appendicular lean soft tissue in young athletes

description: Abstract Magnetic resonance imaging and Computer tomography are gold standards in the measurement of muscle tissue (MT), but are expensive. Dual Energy X-Ray Absorptiometry (DXA) is also costly but safer and allows for the measurement of Appendicular Lean Soft Tissue (ALST), a strong predictor of MT. Alternatively, there are anthropometric models that predict the ALST of Portuguese athletes with low cost/risk that have not been validated in other populations. The aim of this study was to validate anthropometric Portuguese models that predict ALST in young athletes or, if the validation fails, to propose new models. The ALSTDXA of 174 young athletes was determined by DXA. Two anthropometric models (ALSTmod1 and ALSTmod2) measuring ALST among Portuguese athletes were tested. To validate the coefficient of determination, the difference (bias) and concordance correlation coefficient between predicted and actual values were computed. Finally, association between mean and difference of methods was verified. Validation failed and, for this reason, new multiple regression models were proposed and validated using PRESS statistics. The Portuguese models explained ~96% of the ALSTDXA variability. The difference between ALST (-0.7kg) was less than that found for the ALSTmod2 and ALSTDXA limits of agreement from 3.6 to -2.1 and from 6.1 to -1.5kg, respectively. The new models included three predictive equations for ALST. Only ASLTmod1was valid; however, it was prone to bias, depending on the magnitude of ALST values. The newly proposed models present validity with greater concordance (r2PRESS=0.98), lower standard error of estimate (SEEPRESS [kg]=0.91) and more homogeneous predicted extreme values.
DOI: 10.6084/m9.figshare.5734470.v1
title: Validation of anthropometric models in the estimation of appendicular lean soft tissue in young athletes

description: Abstract Magnetic resonance imaging and Computer tomography are gold standards in the measurement of muscle tissue (MT), but are expensive. Dual Energy X-Ray Absorptiometry (DXA) is also costly but safer and allows for the measurement of Appendicular Lean Soft Tissue (ALST), a strong predictor of MT. Alternatively, there are anthropometric models that predict the ALST of Portuguese athletes with low cost/risk that have not been validated in other populations. The aim of this study was to validate anthropometric Portuguese models that predict ALST in young athletes or, if the validation fails, to propose new models. The ALSTDXA of 174 young athletes was determined by DXA. Two anthropometric models (ALSTmod1 and ALSTmod2) measuring ALST among Portuguese athletes were tested. To validate the coefficient of determination, the difference (bias) and concordance correlation coefficient between predicted and actual values were computed. Finally, association between mean and difference of methods was verified. Validation failed and, for this reason, new multiple regression models were proposed and validated using PRESS statistics. The Portuguese models explained ~96% of the ALSTDXA variability. The difference between ALST (-0.7kg) was less than that found for the ALSTmod2 and ALSTDXA limits of agreement from 3.6 to -2.1 and from 6.1 to -1.5kg, respectively. The new models included three predictive equations for ALST. Only ASLTmod1was valid; however, it was prone to bias, depending on the magnitude of ALST values. The newly proposed models present validity with greater concordance (r2PRESS=0.98), lower standard error of estimate (SEEPRESS [kg]=0.91) and more homogeneous predicted extreme values.
DOI: 10.6084/m9.figshare.5734467
title: EVALUATION OF COMPETENCES DEVELOPED IN MEDICINAL CHEMISTRY DISCIPLINE OF STRICTU SENSU POST-GRADUATION: A CLASSROOM EXPERIENCE

description: The knowledge transmission in scientific education demands the formulation of methods capable to insert between student and the study object a nearness, making the student apt to build, from autodidactic posture, solutions that only the professional formation can produce. In this sense, a teaching should develop an effective pedagogy, focusing on knowledge production and its preservation, helping the scientific progress. Guided by these concepts, the educational process can be considered complex and should focus on the quality of the transmitted knowledge, through strategies that ensure the maximum information accessibility. In strictu sensu post-graduation context, some particularities related to the knowledge building and not so only the concepts memorization should have more attention, due to the scientific and teacher training goals. The medicinal chemistry area exhibits the necessity to have a great interlocution with other chemistry disciplines, such as organic chemistry, analytical chemistry and as well as disciplines related to human health. In this sense, the evaluation of learning process becomes, currently, a great challenge for professors that seek to develop the interdisciplinary sense. Considering this reality, in this study, the authors present a classroom experience, where specific competences developed in students of medicinal chemistry discipline was a target of evaluation based on creation of review text. From the results obtained was possible to rethink strategies that can contribute to foster new forms to evaluate the learning process based on competences development.