cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7898750.v1
title: Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway

description: This study investigated the in vitro and in vivo antiproliferative activity of esculetin against hepatocellular carcinoma, and clarified its potential molecular mechanisms. Cell viability was determined by the MTT (tetrazolium) colorimetric assay. In vivo antitumor activity of esculetin was evaluated in a hepatocellular carcinoma mouse model. Seventy-five C57BL/6J mice were implanted with Hepa1-6 cells and randomized into five groups (n=15 each) given daily intraperitoneal injections of vehicle (physiological saline), esculetin (200, 400, or 700 mg·kg-1·day-1), or 5-Fu (200 mg·kg-1·day-1) for 15 days. Esculetin significantly decreased tumor growth in mice bearing Hepa1-6 cells. Tumor weight was decreased by 20.33, 40.37, and 55.42% with increasing doses of esculetin. Esculetin significantly inhibited proliferation of HCC cells in a concentration- and time-dependent manner and with an IC50 value of 2.24 mM. It blocked the cell cycle at S phase and induced apoptosis in SMMC-7721 cells with significant elevation of caspase-3 and caspase-9 activity, but did not affect caspase-8 activity. Moreover, esculetin treatment resulted in the collapse of mitochondrial membrane potential in vitro and in vivo accompanied by increased Bax expression and decreased Bcl-2 expression at both transcriptional and translational levels. Thus, esculetin exerted in vitro and in vivo antiproliferative activity in hepatocellular carcinoma, and its mechanisms involved initiation of a mitochondrial-mediated, caspase-dependent apoptosis pathway.
DOI: 10.6084/m9.figshare.7898729
title: GROWTH OF ATLANTIC FOREST TREES AND THEIR INFLUENCE ON TOPSOIL FERTILITY IN THE SOUTHEASTERN BRAZIL

description: ABSTRACT The aim of our study was to evaluate the dendrometric characteristics of Atlantic Forest trees of 21 years of deployment in the mountain region of Rio de Janeiro, Brazil and their influence on topsoil fertility. We evaluated the survival rates, total heights, and diameters at 1.30 m of all trees in the stand. The characterized soil chemical attributes were pH, P, K, Ca, Mg, Al, and organic matter at 0-0.1 m depth. The averages were compared by Student’s test (p&lt;0.05). While Myroxylon peruiferum and Amburana cearensis had lower survival rates than the other species, Plathymenia reticulata had a larger diameter. All tree species showed diameter growth, though it was larger for Dalbergia nigra, Plathymenia reticulata, Schizolobium parahyba, Esenbeckia leiocarpa, and Lecythis Pisonis. All stands showed medium organic matter content, from 3.3 to 4.1%, and low P. The soils under the Dalbergia nigra and Plathymenia reticulata stands are the most acidic and with low Ca, Mg, and K levels. Our study demonstrated differences in growth patterns and that the species influenced the soil chemical characteristics differently.
DOI: 10.6084/m9.figshare.7898732
title: Echocardiographic Correlation between Right Ventricular Function and Left Atrial Volume

description: Abstract Background: Few reports exist on the relationship of the left ventricular diastolic dysfunction (LVDD) with its most important features including enlargement of the left atrium and left ventricular hypertrophy (LVH), and with the right ventricular (RV) function. Objective: To determine the correlation between the left atrial size and the RV function and dimensions in patients with and without LVDD and LVH. Methods: Fifty patients were included, 25 (40% men) of them with LVDD, aged 67.1 ± 10.6 years (study group) and 25 without LVDD (52% men) aged 49.9 ± 16.3 years (control group). Patients underwent transthoracic echocardiography with evaluation of the left atrial size and volume (LAV), LVDD, LVH, and RV function and dimensions. P-values &lt; 0.05 were considered statistically significant. Results: LAV &gt; 34 mL/m2 and left atrial size &gt; 40 mm were associated with lower absolute values of tricuspid annular plane systolic excursion (TAPSE) and RV lateral S' (p ≤ 0.001, Pearson's correlation coefficient -0.4 and -0.38, respectively) in the study group. Patients in the study group showed higher incidence of LVH (p = 0.02) and greater left atrial diameter (p = 0.03) compared with the control group. In addition, greater left atrial diameter (p = 0.02) and LAV (p = 0.01) values were found in patients with LVDD grade II compared with LVDD grade I. Conclusions: The present study determined, for the first time, the correlation of left atrial enlargement with progressive RV dysfunction in patients with LVDD.
DOI: 10.6084/m9.figshare.7898726
title: The performance of the (U+Th)/He thermochronology laboratory at UNESP Rio Claro, Brazil

description: We have developed the first laboratory for (U-Th-Sm)/He thermochronology measurements in South America. Helium is measured using a high-sensitivity magnetic sector mass spectrometer (GVI-Helix-SFT) and a double focusing single collector inductively coupled plasma mass spectrometer (Thermo Scientific ELEMENT2) is used for U, Th and Sm determinations. Repeated analyses of fragments of Durango fluorapatite crystals yields an average age of 31.6 ± 1.6 Ma (2σ) (n=62). This overlaps the long-term average of Durango fluorapatite measured in laboratories worldwide. The analysis of multiple single apatite crystals of a Precambrian basement sample from Serra do Mar, southeastern Brazil, yields an average He age (60.5 ± 8.7 Ma; n=8) that overlaps that measured in the SUERC laboratory (59.6 ± 3 Ma; n=3). This confirms that the UNESP laboratory is capable of routinely measuring the (U-Th-Sm)/He ages of single apatite crystals.
DOI: 10.6084/m9.figshare.7898732.v1
title: Echocardiographic Correlation between Right Ventricular Function and Left Atrial Volume

description: Abstract Background: Few reports exist on the relationship of the left ventricular diastolic dysfunction (LVDD) with its most important features including enlargement of the left atrium and left ventricular hypertrophy (LVH), and with the right ventricular (RV) function. Objective: To determine the correlation between the left atrial size and the RV function and dimensions in patients with and without LVDD and LVH. Methods: Fifty patients were included, 25 (40% men) of them with LVDD, aged 67.1 ± 10.6 years (study group) and 25 without LVDD (52% men) aged 49.9 ± 16.3 years (control group). Patients underwent transthoracic echocardiography with evaluation of the left atrial size and volume (LAV), LVDD, LVH, and RV function and dimensions. P-values &lt; 0.05 were considered statistically significant. Results: LAV &gt; 34 mL/m2 and left atrial size &gt; 40 mm were associated with lower absolute values of tricuspid annular plane systolic excursion (TAPSE) and RV lateral S' (p ≤ 0.001, Pearson's correlation coefficient -0.4 and -0.38, respectively) in the study group. Patients in the study group showed higher incidence of LVH (p = 0.02) and greater left atrial diameter (p = 0.03) compared with the control group. In addition, greater left atrial diameter (p = 0.02) and LAV (p = 0.01) values were found in patients with LVDD grade II compared with LVDD grade I. Conclusions: The present study determined, for the first time, the correlation of left atrial enlargement with progressive RV dysfunction in patients with LVDD.
DOI: 10.6084/m9.figshare.7898729.v1
title: GROWTH OF ATLANTIC FOREST TREES AND THEIR INFLUENCE ON TOPSOIL FERTILITY IN THE SOUTHEASTERN BRAZIL

description: ABSTRACT The aim of our study was to evaluate the dendrometric characteristics of Atlantic Forest trees of 21 years of deployment in the mountain region of Rio de Janeiro, Brazil and their influence on topsoil fertility. We evaluated the survival rates, total heights, and diameters at 1.30 m of all trees in the stand. The characterized soil chemical attributes were pH, P, K, Ca, Mg, Al, and organic matter at 0-0.1 m depth. The averages were compared by Student’s test (p&lt;0.05). While Myroxylon peruiferum and Amburana cearensis had lower survival rates than the other species, Plathymenia reticulata had a larger diameter. All tree species showed diameter growth, though it was larger for Dalbergia nigra, Plathymenia reticulata, Schizolobium parahyba, Esenbeckia leiocarpa, and Lecythis Pisonis. All stands showed medium organic matter content, from 3.3 to 4.1%, and low P. The soils under the Dalbergia nigra and Plathymenia reticulata stands are the most acidic and with low Ca, Mg, and K levels. Our study demonstrated differences in growth patterns and that the species influenced the soil chemical characteristics differently.
DOI: 10.6084/m9.figshare.7898723
title: Role of mitochondrial ATP-sensitive potassium channel-mediated PKC-ε in delayed protection against myocardial ischemia/reperfusion injury in isolated hearts of sevoflurane-preconditioned rats

description: This study aimed to determine the role of mitochondrial adenosine triphosphate-sensitive potassium (mitoKATP) channels and protein kinase C (PKC)-ε in the delayed protective effects of sevoflurane preconditioning using Langendorff isolated heart perfusion models. Fifty-four isolated perfused rat hearts were randomly divided into 6 groups (n=9). The rats were exposed for 60 min to 2.5% sevoflurane (the second window of protection group, SWOP group) or 33% oxygen inhalation (I/R group) 24 h before coronary occlusion. The control group (CON) and the sevoflurane group (SEVO) group were exposed to 33% oxygen and 2.5% sevoflurane for 60 min, respectively, without coronary occlusion. The mitoKATP channel inhibitor 5-hydroxydecanoate (5-HD) was given 30 min before sevoflurane preconditioning (5-HD+SWOP group). Cardiac function indices, infarct sizes, serum cardiac troponin I (cTnI) concentrations, and the expression levels of phosphorylated PKC-ε (p-PKC-ε) and caspase-8 were measured. Cardiac function was unchanged, p-PKC-ε expression was upregulated, caspase-8 expression was downregulated, cTnI concentrations were decreased, and the infarcts were significantly smaller (P&lt;0.05) in the SWOP group compared with the I/R group. Cardiac function was worse, p-PKC-ε expression was downregulated, caspase-8 expression was upregulated, cTnI concentration was increased and infarcts were larger in the 5-HD+SWOP group (P&lt;0.05) compared with the SWOP group. The results suggest that mitoKATP channels are involved in the myocardial protective effects of sevoflurane in preconditioning against I/R injury, by regulating PKC-ε phosphorylation before ischemia, and by downregulating caspase-8 during reperfusion.
DOI: 10.6084/m9.figshare.7898726.v1
title: The performance of the (U+Th)/He thermochronology laboratory at UNESP Rio Claro, Brazil

description: We have developed the first laboratory for (U-Th-Sm)/He thermochronology measurements in South America. Helium is measured using a high-sensitivity magnetic sector mass spectrometer (GVI-Helix-SFT) and a double focusing single collector inductively coupled plasma mass spectrometer (Thermo Scientific ELEMENT2) is used for U, Th and Sm determinations. Repeated analyses of fragments of Durango fluorapatite crystals yields an average age of 31.6 ± 1.6 Ma (2σ) (n=62). This overlaps the long-term average of Durango fluorapatite measured in laboratories worldwide. The analysis of multiple single apatite crystals of a Precambrian basement sample from Serra do Mar, southeastern Brazil, yields an average He age (60.5 ± 8.7 Ma; n=8) that overlaps that measured in the SUERC laboratory (59.6 ± 3 Ma; n=3). This confirms that the UNESP laboratory is capable of routinely measuring the (U-Th-Sm)/He ages of single apatite crystals.
DOI: 10.6084/m9.figshare.7898708
title: Literature review of audiovisual practices in Nursing education

description: ABSTRACT Objective: to carry out a literature review to characterize the production in the Nursing area about the use of movies and videos in Nursing education, related to underlying communication assumptions and contents taught. Method: integrative bibliographic review carried out in the PUBMED databases, CAPES journal portal, and SCIELO online library, with studies from 2007 to 2016. Results: 36 articles were included, in which audiovisual resources are part of the research subject. There are predominance of validation and effectiveness studies, in which audiovisual resources are used mostly as a reproduction of reality, mainly to develop clinical skills, with the purpose of illustrating procedures or bringing students closer to real contexts of action. Final considerations: the results point to a weak theoretical support of most of the studies. It is suggested that more empirical research be conducted to offer more consistent contributions to Nursing teaching.
DOI: 10.6084/m9.figshare.7898711
title: Analysis of strengthening procedures of reinforced concrete highway bridges: a brazilian case study

description: Abstract The Brazilian federal highway system is largely made up of reinforced concrete (RC) bridges built prior to 1984. Thus, these bridges have incompatible geometry and loading carrying capacity with nowadays traffic. In this scenario, the goal of this investigation was the evaluation of the widening and strengthening procedures used in these RC bridges. The study also includes comparing their performance with the respective new bridges built next to them, which received new highway lanes. This comparison is adequate, considering that, both bridges are inserted in the same environment, are subjected to the same traffic characteristics and have similar spans and structural systems. The results obtained allow us to know the effectiveness of the widening and strengthening interventions carried out, from the point of view of durability, contributing to the improvement of future rehabilitation design for reinforced concrete highway bridges.
DOI: 10.6084/m9.figshare.7898717
title: Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

description: Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3−. Compared with benign lesions, malignant lesions had less NR1I3+ tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice.
DOI: 10.6084/m9.figshare.7898723.v1
title: Role of mitochondrial ATP-sensitive potassium channel-mediated PKC-ε in delayed protection against myocardial ischemia/reperfusion injury in isolated hearts of sevoflurane-preconditioned rats

description: This study aimed to determine the role of mitochondrial adenosine triphosphate-sensitive potassium (mitoKATP) channels and protein kinase C (PKC)-ε in the delayed protective effects of sevoflurane preconditioning using Langendorff isolated heart perfusion models. Fifty-four isolated perfused rat hearts were randomly divided into 6 groups (n=9). The rats were exposed for 60 min to 2.5% sevoflurane (the second window of protection group, SWOP group) or 33% oxygen inhalation (I/R group) 24 h before coronary occlusion. The control group (CON) and the sevoflurane group (SEVO) group were exposed to 33% oxygen and 2.5% sevoflurane for 60 min, respectively, without coronary occlusion. The mitoKATP channel inhibitor 5-hydroxydecanoate (5-HD) was given 30 min before sevoflurane preconditioning (5-HD+SWOP group). Cardiac function indices, infarct sizes, serum cardiac troponin I (cTnI) concentrations, and the expression levels of phosphorylated PKC-ε (p-PKC-ε) and caspase-8 were measured. Cardiac function was unchanged, p-PKC-ε expression was upregulated, caspase-8 expression was downregulated, cTnI concentrations were decreased, and the infarcts were significantly smaller (P&lt;0.05) in the SWOP group compared with the I/R group. Cardiac function was worse, p-PKC-ε expression was downregulated, caspase-8 expression was upregulated, cTnI concentration was increased and infarcts were larger in the 5-HD+SWOP group (P&lt;0.05) compared with the SWOP group. The results suggest that mitoKATP channels are involved in the myocardial protective effects of sevoflurane in preconditioning against I/R injury, by regulating PKC-ε phosphorylation before ischemia, and by downregulating caspase-8 during reperfusion.
DOI: 10.6084/m9.figshare.7898702
title: Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence

description: Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was effective for the alcoholism in dogs. The morphology of hippocampal neurons was evaluated using hematoxylin-eosin staining. The number and morphological features of BDNF, TrkB and p75NTR-positive neurons in the dentate gyrus (DG), and the CA1, CA3 and CA4 regions of the hippocampus were observed using immunohistochemistry. One-way ANOVA was used to determine differences in BDNF, TrkB and p75NTR expression. BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG&gt;CA1&gt;CA3&gt;CA4). Expression levels of both BDNF and TrkB were decreased in chronic alcoholism, and increased after abstinence. The CA4 region appeared to show the greatest differences. Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.
DOI: 10.6084/m9.figshare.7898705
title: Neoadjuvant chemotherapy with trastuzumab in HER2-positive breast cancer: pathologic complete response rate, predictive and prognostic factors

description: The purpose of this study was to retrospectively review the pathologic complete response (pCR) rate from patients (n=86) with stage II and III HER2-positive breast cancer treated with neoadjuvant chemotherapy at our institution from 2008 to 2013 and to determine possible predictive and prognostic factors. Immunohistochemistry for hormone receptors and Ki-67 was carried out. Clinical and pathological features were analyzed as predictive factors of response to therapy. For survival analysis, we used Kaplan-Meier curves to estimate 5-year survival rates and the log-rank test to compare the curves. The addition of trastuzumab to neoadjuvant chemotherapy significantly improved pCR rate from 4.8 to 46.8%, regardless of the number of preoperative trastuzumab cycles (P=0.0012). Stage II patients achieved a higher response rate compared to stage III (P=0.03). The disease-free and overall survivals were not significantly different between the group of patients that received trastuzumab in the neoadjuvant setting (56.3 and 70% at 5 years, respectively) and the group that initiated it post-operatively (75.8 and 88.7% at 5 years, respectively). Axillary pCR post neoadjuvant chemotherapy with trastuzumab was associated with reduced risk of recurrence (HR=0.34; P=0.03) and death (HR=0.21; P=0.02). In conclusion, we confirmed that trastuzumab improves pCR rates and verified that this improvement occurs even with less than four cycles of the drug. Hormone receptors and Ki-67 expressions were not predictive of response in this subset of patients. Axillary pCR clearly denotes prognosis after neoadjuvant target therapy and should be considered to be a marker of resistance, providing an opportunity to investigate new strategies for HER2-positive treatment.
DOI: 10.6084/m9.figshare.7898711.v1
title: Analysis of strengthening procedures of reinforced concrete highway bridges: a brazilian case study

description: Abstract The Brazilian federal highway system is largely made up of reinforced concrete (RC) bridges built prior to 1984. Thus, these bridges have incompatible geometry and loading carrying capacity with nowadays traffic. In this scenario, the goal of this investigation was the evaluation of the widening and strengthening procedures used in these RC bridges. The study also includes comparing their performance with the respective new bridges built next to them, which received new highway lanes. This comparison is adequate, considering that, both bridges are inserted in the same environment, are subjected to the same traffic characteristics and have similar spans and structural systems. The results obtained allow us to know the effectiveness of the widening and strengthening interventions carried out, from the point of view of durability, contributing to the improvement of future rehabilitation design for reinforced concrete highway bridges.
DOI: 10.6084/m9.figshare.7898717.v1
title: Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

description: Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3−. Compared with benign lesions, malignant lesions had less NR1I3+ tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice.