DOI: 10.5061/dryad.2mh70m2
title: Data from: Cytochrome P450 and O-methyltransferase catalyze the ﬁnal steps in the biosynthesis of the anti-addictive alkaloid ibogaine from Tabernanthe iboga

description: Monoterpenoid indole alkaloids (MIAs) are a large (ca. 3000 members) and
 structurally diverse class of metabolites restricted to a limited number
 of plant families in the order Gentianales. Tabernanthe iboga or iboga
 (Apocynaceae) is native to western equatorial Africa and has been used in
 traditional medicine for centuries. Howard Lotsof is credited with
 bringing iboga to the attention of Western medicine through his accidental
 discovery that iboga can alleviate opioid withdrawal symptoms. Since this
 observation, iboga has been investigated for its use in the general
 management of addiction. We were interested in elucidating ibogaine
 biosynthesis to understand the unique reaction steps en route to ibogaine.
 Furthermore, because ibogaine is currently sourced from plant material,
 these studies may help improve the ibogaine supply chain through synthetic
 biology approaches. Here we used next-generation sequencing to generate
 the ﬁrst iboga transcriptome, and leveraged homology guided gene discovery
 to identify the penultimate hydroxylase and ﬁnal O-methyltransferase steps
 in ibogaine biosynthesis, herein named ibogamine 10-hydroxylase (I10H) and
 noribogaine 10-O-methyltransferase (N10OMT). Heterologous expression in
 Saccharomyces cerevisiae (I10H) or Escherichia coli (N10OMT) and
 incubation with putative precursors, along with HPLC–MS analysis, conﬁrmed
 the predicted activities of both enzymes. Moreover, high expression levels
 of their transcripts were detected in ibogaine-accumulating plant tissues.
 These discoveries coupled with our publicly available iboga transcriptome
 will contribute to additional gene discovery eﬀorts and could lead to the
 stabilization of the global ibogaine supply chain and to the development
 of ibogaine as a treatment for addiction.
description: 1_T_iboga_1.fq.gzThese are illumina reads. The files T_iboga_1.fq.gz.001 and T_iboga_1.fq.gz.002 must be joined into a single file before decompressing. Join the files using the following command indicated for your operating system. Linux/Mac OS X: cat T_iboga_1.fq.gz.* &gt; T_iboga_1.fq.gz Windows: copy /b T_iboga_1.fq.gz.001 + T_iboga_1.fq.gz.002 T_iboga_1.fq.gzT_iboga_1.fq.gz.0012_T_iboga_1.fq.gzThe files T_iboga_1.fq.gz.001 and T_iboga_1.fq.gz.002 must be joined into a single file before decompressing. Join the files using the following command indicated for your operating system. Linux/Mac OS X: cat T_iboga_1.fq.gz.* &gt; T_iboga_1.fq.gz Windows: copy /b T_iboga_1.fq.gz.001 + T_iboga_1.fq.gz.002 T_iboga_1.fq.gzT_iboga_1.fq.gz.002T_iboga_2.fq.gzThe files T_iboga_2.fq.gz.001 and T_iboga_2.fq.gz.002 must be joined into a single file before decompressing. Join the files using the following command indicated for your operating system. Linux/Mac OS X: cat T_iboga_2.fq.gz.* &gt; T_iboga_2.fq.gz Windows: copy /b T_iboga_2.fq.gz.001 + T_iboga_2.fq.gz.002 T_iboga_2.fq.gzT_iboga_2.fq.gzThe files T_iboga_2.fq.gz.001 and T_iboga_2.fq.gz.002 must be joined into a single file before decompressing. Join the files using the following command indicated for your operating system. Linux/Mac OS X: cat T_iboga_2.fq.gz.* &gt; T_iboga_2.fq.gz Windows: copy /b T_iboga_2.fq.gz.001 + T_iboga_2.fq.gz.002 T_iboga_2.fq.gz