cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0kw5f0z
title: CIL:36566, Xenopus laevis, fertilized egg. In Cell Image Library

description: This image is part of a large data set of Xenopus laevis eggs imaged at various times post fertilization (the first number of the file name corresponds minutes, eg 30_2 is 30 min post fertilization). The embryos are stained for alpha-tubulin (green) using a directly labeled B-5-1-2 antibody and DNA (red).
description: Images collected on a Zeiss LSM 710 confocal with a 10X 0.3 NA objective using 561 and 633 laser lines.
description: Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; distribution of a specific protein
Visualization methods: labeled primary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0cv4gpr
title: CIL:36596, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Striated bands in a transverse ridge (in which kinetodesmal fibers are lacking) at high magnification. The longer band has seven stripes rather than five as in the shorter band. This suggests three regions of overlap rather than two. The filaments may have a set length so that for longer distances two sets of overlapping filaments may be needed to span the distance between the filaments arising from the epiplasm. TEM taken on 2/21/69 by R. Allen with Philips 300 operating at 60kV. Neg. 47,100X. Bar = 0.1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39307) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j03f4nm9
title: CIL:36630, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Protozoa, including P. multimicronucleatum, unlike most cells, have tubular cristae in their mitochondria. Tubular cristae, however, do occur occasionally in higher organisms such as in the lutein cells and testes interstitial cells of humans. Mitochondria are particularly numerous in the cortical region of paramecia. Collidine-buffered fixative. This image includes trichocysts that have expanded internally during fixation. TEM taken on 12/18/68 by R. Allen with Philips 300 operating at 80kV. Neg. 16,000X. Bar = 0.5µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j00k27hp
title: CIL:36594, Danio rerio, embryo. In Cell Image Library

description: Images were collected on a Zeiss LSM 710 with a 20X 1.0 NA objective.
description: 4D video of zebrafish embryo mitosis and cytokinesis. This time lapse video of z-series images was taken of a zebrafish embryo expressing EMTB-3XGFP (a microtubule marker) taken shortly after fertilization.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0b27t6h
title: CIL:36593, Xenopus laevis, fertilized embryo. In Cell Image Library

description: Images were collected with a dissecting microscope.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: bright-field microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in intrinsic optical density
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Time series of images of fertilized Xenopus laevis embryos with their vitellin membrane removed. Embryos were incubated plus/minus the actin depolymerizer cytochalsin B.
DOI: 10.6075/j0wd3z62
title: CIL:36635, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Isolated mitochondria that have been quick-frozen, fractured and deep-etched show some space between the inner and outer membranes. Arcs of tubules, where they are tangentially fractured, show the helically arrayed zipper on the outer margin of the curved tubular cristae. A pair of particles can be seen on the cross-fractured tubules. These may be bound to transmembrane subunits. Such complexes are thought to represent the F1F0 synthases. TEM taken on 5/11/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 40,500X. Bar = 0.1µm. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40504) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/). Published in J. Cell Biol. 108:2233-2240, 1989. Adapted with permission.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0z60n1h
title: CIL:36610, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: In this image a portion of the true surface of the plasma membrane is exposed where the culture medium has been etched away. The IMPs of the plates can also be viewed less distinctly on the true surface of the cell. The edge of the fractured and removed E leaflet is visible. Fractured doublets of the axoneme of a cilium lie nearby. TEM taken on 6/4/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2 µm. There is a high resolution version of this image in the library (CIL:39315) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j08c9v65
title: CIL:36607, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: In quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing between rows of 25nm. These plaques remain in the P-fracture face of the plasma membrane and are found mostly on the anterior ventral surface of the cell near the anterior suture. Their function is not known. For discussion see Allen, J. Ultrastruc. Res. 63:64-78, 1978 and Hufnagel, Micros. Res. Tech. 22:225-264, 1992. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39314) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j04b307d
title: CIL:36612, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A row of large IMPs bordering parts of the plates are visible. Two parentheses-shaped empty trichocyst docking sites are also present and one ring surrounding pits where the rosette IMPs have been removed. TEM taken on 5/9/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39316) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j05q4v03
title: CIL:36602, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39312) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Longitudinal section of a striated band lying superficially to an arm of the infraciliary lattice but not contacting it. A post is seen at the junction of a branch in the infraciliary lattice. TEM taken on 5/21/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 27,750X. Bar = 0.2µm.
DOI: 10.6075/j09c6wc0
title: CIL:36605, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Freeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous plates of IMPs in the P-face of the plasma membrane. Rings and rosette IMPs at the trichocyst exocytic sites are also seen. TEM taken on 10/2/80 by R. Allen with HU 11A operating at 75kV. Neg. 10,000X. Bar = 1µm. There is a high resolution version of this image in the library (CIL:39313) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j06972g5
title: CIL:36598, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Cross sections of striated bands and infraciliary lattice arms. Filaments of the striated bands are less densely packed than the infraciliary lattice. Kinetodesmal fibers and postciliary microtubules are also cross sectioned and are seen to come to lie near each other in the lateral ridges. A linkage would provide additional strength to the kineties. The observer is looking at a cross section of the cell from the posterior toward the anterior of the cell. TEM taken on 6/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 36,500X. Bar = 0.1µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39309) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0vm4b59
title: CIL:36597, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Striated band spanning the base of a longitudinal ridge (kinetodesmal fibers are seen in the ridge in cross section). The epiplasm appears absent from the top of the ridge or is replaced by two thickenings connected by thin filaments. Observing from outside the cell looking from the posterior toward the anterior direction. TEM taken on 12/9/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 37,750X. Bar = 0.1µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39308) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0rj4h49
title: CIL:36634, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Quick-freeze deep-etch image of an unfixed and fractured mitochondrion. The tubular cristae average 50nm in diameter. Each cristae bears a helically arranged double row of particles with the particles spaced at 12 nm along this zipper-like arrangement. The cristae themselves assume a helical shape and the zipper array remains on the outside curve of the helix. In in situ fractures the 2 enclosing membranes lie close together. Crystals of unknown composition are often found in the mitochondria. TEM taken on 2/15/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 40,500X. Bar = 0.1µm. Published in J. Cell Biol. 108:2233-2240, 1989. Adapted with permission. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40503) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.5281/zenodo.15614581
title: BiotXBench

description: The BiotXBench project (Benchmarking Biotic Interactions) aims to benchmark the accuracy of automatic annotations applied to digital library collections in life and health sciences. By focusing on manually and automatically generated annotations from PubMed Central (PMC, a large repository of research articles), the project will evaluate the performance and reliability of these annotations.
DOI: 10.5281/zenodo.15614580
title: BiotXBench

description: The BiotXBench project (Benchmarking Biotic Interactions) aims to benchmark the accuracy of automatic annotations applied to digital library collections in life and health sciences. By focusing on manually and automatically generated annotations from PubMed Central (PMC, a large repository of research articles), the project will evaluate the performance and reliability of these annotations.
DOI: 10.6075/j0kd1wv6
title: CIL:36617, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: Quick-freeze deep-etch of granulo-fibrillar material at the crests of the ridges. This material is composed of thinner and shorter filaments that, like the striated bands, appear to bind to the membrane. The fracture plane also exposed the cell‚Äôs exterior. TEM taken on 6/4/91 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39321) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j01z43c3
title: CIL:36615, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The infraciliary lattice in this quick-freeze deep-etched image consists of a meshwork of branching fibers composed of many subfibers. Basal bodies and trichocyst tips extend through the openings of the mesh. Individual metal-coated fibrils of the mesh average 11 nm in diameter and are closely interlaced and cross-linked to other fibrils. TEM taken on 5/27/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm. There is a high resolution version of this image in the library (CIL:39319) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed