DOI: 10.5061/dryad.1jwstqjrq
title: Data from: Colonization of marginal host plants by Callosobruchus seed beetles (Coleoptera: Chrysomelidae): effects of geographic source and genetic admixture

description: The ability to adapt to a novel host plant may vary among insect
 populations with different genetic histories, and colonization of a
 marginal host may be facilitated by genetic admixture of disparate
 populations. We assembled populations of the seed beetle, Callosobruchus
 maculatus, from four continents, and compared their ability to infest two
 hosts, lentil and pea. We also formed two cross-continent hybrids (Africa
 x N.A and Africa x S.A.). In pre-selection assays, survival was only ~3%
 in lentil and ~40% in pea. For three populations per line, colonization
 success on lentil was measured as cumulative exit holes after 75-175 days.
 On pea, we estimated the change in larval survival after five generations
 of selection. Females in all lines laid few eggs on lentil, and survival
 of F1 larvae was uniformly &lt;5%. Subsequently, however, the lines
 diverged considerably in population growth. Performance on lentil was
 highest in the Africa x N.A. hybrid, which produced far more adults (mean
 &gt;11000) than either parental line. At the other extreme, Asian
 populations on lentil appeared to have gone extinct. The Africa x N.A.
 line also exhibited the highest survival on pea, and again performed
 better than either parent line. However, no line displayed a rapid
 increase in survival on pea, as is sometimes observed on lentil. Our
 results demonstrate that geographic populations can vary substantially in
 their responses to the same novel resource. In addition, genetic
 admixtures (potentially caused by long-distance transport of infested
 seeds) may facilitate colonization of an initially poor host.
description: We
 assembled populations of the cowpea seed beetle, <i>Callosobruchus
 maculatus</i>, from four continents, and compared their ability to
 infest two novel hosts, lentil and pea. We also formed cross-continent
 hybrids (Africa x N.A and Africa x S.A.) to determine whether genetic
 admixture could facilitate adaptation. In pre-selection assays, survival
 was only ~3% in lentil and ~40% in pea. For three replicate populations
 per line, colonization success on lentil was measured as cumulative exit
 holes after 75 to 175 days. On pea, we instead estimated the change in
 larval survival after five discrete generations of selection.
description: This data submission includes two data files, two R scripts, and
 two associated JAGS models: 1. lentil_exit_holes.csv =
 data file with lentil exit hold data. Columns denote the source population
 or cross type (LINE), how many seesds had 0, 1, 2, 3 or 4 exit holes, and
 the timing of the assay in days. 2. pea_exit_f5.csv =
 =data file  with pea assay data. Columns denote the source popualtion or
 cross type (Line), replicate, number of seeds and number of beetles that
 successfully emerged (Emerge). 3. commands_simple.R = R
 script to fit the Bayesian model for the lentil analysis.
 4. pea_survival.R = R script to fit the Bayesian model for the
 pea survival analysis. 5. model_simple.txt = JAGS
 specification of the Bayesian model for the lentil analysis.
 6. pea_model = JAGS specification of the Bayesian model for the
 pea survival analysis.