cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0154gb8
title: CIL:40367, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library

description: Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images ca ptured with a MicroMax cooled CCD camera.
Images were captured every 2 sec and are displayed at 7.5 frames/sec. 
See also:N Rusan and P Wadsworth 2005. Centrosome fragments and microtubules are transported asymmetrically away from division plane in mitosis.J Cell Biol 168:21-28.
description: Time lapse movie of a porcine kidney LLCPK1 cell stably expressing GFP-alpha tubulin. The movie shows mictotubule elongation toward the cell cortex following anaphase onset followed by resumption of dynamic instability. 
 A time lapse movie created from the series is grouped with it.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed
DOI: 10.6075/j0db816r
title: CIL:40366, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library

description: See: KJ Salaycik et al. 2005. Quantification of microtubule growth and dynamics in wound-edge cells. J Cell Sci 118:4113-4122.
http://jcs.biologists.org/content/118/18/4113.long
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed
description: Time series stack of LLCPK cells stably expressing GFP-EB1, a microtubule tip binding protein. GFP-EB1 fluorescence is seen in microtubules and centrosomes which appear to act as microtubule nucleation sites.
 A movie prepared from the stack is included in this group of images.
DOI: 10.6075/j0639p4t
title: CIL:40361, Rattus norvegicus, oligodendrocyte. In Cell Image Library

description: Rattus norvegicus oligodendrocytes purified from P2 cortex. Cells were fixed on glass coverslips with 4% PFA and immunostained with Phalloidin-FITC (green), alpha-tubulin primary ab./Rhodamine secondary ab (red), RIP (oligodendrocyte marker) primary ab/Cy5 secondary ab (blue), and DAPI (nuclear marker also in blue). Photographed using Leica DMI4000B microscope and 63x HCX PL APO OIL Objective. Voxel size 0.1um.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: unprocessed raw data
description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6075/j0vd6xs3
title: CIL:40357, Rhododendron, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Rhododendron pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j0jd4wzc
title: CIL:40210, Mus musculus, astrocyte of the hippocampus. In Cell Image Library

description: Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).
description: Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 24th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed
DOI: 10.6075/j0t43t61
title: CIL:40187, Homo sapiens. In Cell Image Library

description: Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Parameter imaged: electron density
Data qualification: Processed
description: ReAsH-labeled cells were fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate, pH 7.4 at 4°C. After 20 min, the cells were rinsed in 0.1 M cacodylate pH 7.4 and incubated for 30-45 min with blocking buffer (10 mM KCN, 10 mM aminotriazole, 0.01% hydrogen peroxide/50 mM glycine in 0.1 M cacodylate pH 7.4). This buffer was then replaced with 1 mg/ml diaminobenzidine (DAB) in blocking buffer, and photoconversion was performed using intense illumination (75 W xenon arc lamp without neutral density filters) focused through the microscope objective. Rinsing and further preparation of the sample for transmission electron microscopy were performed as described in Gaietta et al. (2002). Sections were stained with uranyl acetate prior to acquiring tilt images.
description: Single computed slice through a double tilt electron tomogram of a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form "onion skin" sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Lichtenstein et al. The cytoplasmic accumulations of the cataract-associated mutant, Connexin50P88S, are long-lived and form in the endoplasmic reticulum Exp Eye Res. 2009 Mar;88(3):600-9. PMID: 19073179
DOI: 10.6075/j0v124x2
title: CIL:40184, Homo sapiens, pyramidal cell, neuron of cerebral cortex. In Cell Image Library

description: Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Data qualification: Processed
description: Single image from a serial section electron microscopic reconstruction of the cell body of a cortical pyramidal neuron from a biopsy sample obtained from the cortex of a patient with Alzheimer's disease. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Ellisman et al. Neuronal fibrillar cytoskeleton and endomembrane system organization in Alzheimers disease, In Alteration of the Neuronal Cytoskeleton in Aging and Dementia, GP Perry (Ed,).pp, 61-73, Plenum Publ, Corp, New York, 1988.
DOI: 10.6075/j0xs5vhq
title: CIL:40188, Homo sapiens, pyramidal cell, neuron of cerebral cortex. In Cell Image Library

description: Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Data qualification: Processed
description: Electron micrograph of paired helical filaments in the cell body of a 0.25 um thick section through a cortical pyramidal neuron from the brain of a patient with Alzheimer's disease. The specimen was prepared from biopsy material obtained several decades ago. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Ellisman et al. Neuronal fibrillar cytoskeleton and endomembrane system organization in Alzheimers disease, In Alteration of the Neuronal Cytoskeleton in Aging and Dementia, GP Perry (Ed,).pp, 61-73, Plenum Publ, Corp, New York, 1988.
description: Single image from a serial section electron microscopic reconstruction of the cell body of a cortical pyramidal neuron from a biopsy sample obtained from the cortex of a patient with Alzheimer's disease. The specimen was prepared from biopsy material obtained several decades ago. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Ellisman et al. Neuronal fibrillar cytoskeleton and endomembrane system organization in Alzheimers disease, In Alteration of the Neuronal Cytoskeleton in Aging and Dementia, GP Perry (Ed,).pp, 61-73, Plenum Publ, Corp, New York, 1988.
DOI: 10.6075/j0tx3fhf
title: CIL:40201, Mus musculus, astrocyte of the hippocampus. In Cell Image Library

description: Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed
description: Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 17th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.
DOI: 10.6075/j0xw4j6m
title: CIL: 50684, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 1.5_Cell_1_Stereocilia_Segmentation. In Cell Image Library

description: Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
1.5_Cell_1_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results (2.43×2.43×15 nm³ voxel size). Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview. The file contains 348 images, as all the planes that contained no segmentation data were removed. 
This file is a part of Dataset 1, comprised of files related to the Cell #1 of current study. The numbering order of the files reflects the order of operations performed during the analysis.
description: FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
description: Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)
Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads
DOI: 10.6075/j0p55nnn
title: CIL:40209, Mus musculus, astrocyte of the hippocampus. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed
description: Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 9th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.
description: Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).
DOI: 10.6075/j0xp753s
title: CIL:40208, Mus musculus, astrocyte of the hippocampus. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed
description: Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).
description: Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 10th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.
DOI: 10.6075/j0kd1z18
title: CIL:40206, Mus musculus, astrocyte of the hippocampus. In Cell Image Library

description: Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 12th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed
description: Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).
DOI: 10.6075/j0668dbv
title: CIL:40204, Mus musculus, astrocyte of the hippocampus. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed
description: Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 14th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.
description: Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).
DOI: 10.6075/j0zp468m
title: CIL:40198, Drosophila melanogaster. In Cell Image Library

description: Mitochondria were isolated from Drosophila cells as described by Echalier [77]. For electron tomography, cells were fixed in 2% parafomaldehyde and 2.5% glutaraldehyde in 0.1 M sodium cacodylate, pH 7.4, post-fixed in 1% osmium tetroxide with 0.8% potassium ferrocyanide in sodium cacodylate buffer, stained with 2% uranyl acetate, dehydrated in a graded series of ethanol, and embedded in Durcupan ACM resin. Sections were cut with a thickness of approximately 250 nm from blocks exhibiting well-preserved ultrastructure. These sections were stained for 30 min in 2% aqueous uranyl acetate, followed by 30 min in lead salts. Fiducial cues consisting of 20-nm colloidal gold particles were deposited on both sides of the section.
description: Single tilt image of a 250 um thick section of a Drosophila S2 cell infected with flock house virus (FHV-1) imaged with intermediate voltage electron microscopy, taken at zero degree tilt. The dark specks are colloidal gold particles that have been applied to the surfaces of the section to serve as fiducial marks for subsequent alignment. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Kopek et al. Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle. PLoS Biol. 2007 Sep;5(9):e220. PMID: 17696647
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; osmium tetroxide fixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Parameter imaged: electron density
Visualization methods: uranyl salt
Data qualification: Processed