DOI: 10.5061/dryad.5hqbzkhgj
title: Data from: Disruption of the CRF1 receptor eliminates morphine-induced sociability deficits and firing of oxytocinergic neurons in male mice

description: Substance-induced social behavior deficits dramatically worsen the
 clinical outcome of substance use disorders; yet, the underlying
 mechanisms remain poorly understood. Herein, we investigated the role for
 the corticotropin-releasing factor receptor 1 (CRF1) in the acute
 sociability deficits induced by morphine and the related activity of
 oxytocin (OXY)- and arginine-vasopressin (AVP)-expressing neurons of the
 paraventricular nucleus of the hypothalamus (PVN). For this purpose, we
 used both the CRF1 receptor-preferring antagonist compound antalarmin and
 the genetic mouse model of CRF1 receptor-deficiency. Antalarmin completely
 abolished sociability deficits induced by morphine in male, but not in
 female, C57BL/6J mice. Accordingly, genetic CRF1 receptor-deficiency
 eliminated morphine-induced sociability deficits in male mice. Ex vivo
 electrophysiology studies showed that antalarmin also eliminated
 morphine-induced firing of PVN neurons in male, but not in female,
 C57BL/6J mice. Likewise, genetic CRF1 receptor-deficiency reduced
 morphine-induced firing of PVN neurons in a CRF1 gene expression-dependent
 manner. The electrophysiology results consistently mirrored the behavioral
 results, indicating a link between morphine-induced PVN activity and
 sociability deficits. Interestingly, in male mice antalarmin abolished
 morphine-induced firing in neurons co-expressing OXY and AVP, but not in
 neurons expressing only AVP. In contrast, in female mice antalarmin did
 not affect morphine-induced firing of neurons co-expressing OXY and AVP or
 only OXY, indicating a selective sex-specific role for the CRF1 receptor
 in opiate-induced PVN OXY activity. The present findings demonstrate a
 major, sex-linked, role for the CRF1 receptor in sociability deficits and
 related brain alterations induced by morphine, suggesting new therapeutic
 strategy for opiate use disorders.
description: # Disruption of the CRF1 receptor eliminates morphine-induced sociability
 deficits and firing of oxytocinergic neurons in male mice Description of
 the file and data structure: This dataset consists of a data file
 named: Piccin_et_al._eLife_2024_for_Dryad **Table 1 – 3CH C57 males** This
 tab contains the behavioral data reported in **Figure 1B** and **Figure 1
 – figure supplement 1A** “3CH” stands for “three-chamber”, “C57” stands
 for “C57BL/6“ “subject” = ID number of the subject animal “pretreat” =
 pre-treatement received (either “veh” = vehicle or “anta” = antalarmin 20
 mg/kg p.o.) “treat” = treatment received (either “sal” = saline or “mor” =
 morphine 2.5 mg/kg i.p.) “P” stands for “Phase”, “S” stands for “Side”
 “P1_S1” = time spent (sec) on the mouse half-chamber during the
 habituation phase (empty) “P1_S2” = time spent (sec) on the object
 half-chamber during the habituation phase (empty) “P2_S1” = time spent
 (sec) on the mouse half-chamber during the sociability phase “P2_S2” =
 time spent (sec) on the object half-chamber during the sociability phase
 “soc ratio” = % of time spent with the mouse, calculated as
 [P2_S1/(P2_S1+P2_S2)]*100 “loco hab” = distance travelled (meters) by the
 subject mouse during the habituation phase “loco soc” = distance travelled
 (meters) by the subject mouse during the sociability phase In red and bold
 are noted the mean and the SEM of the data in the corresponding column
 **Table 2 – 3CH C57 females** This tab contains the behavioral data
 reported in **Figure 1C** and **Figure 1 – figure supplement 1B** “3CH”
 stands for “three-chamber”, “C57” stands for “C57BL/6“ “subject” = ID
 number of the subject animal “pretreat” = pre-treatement received (either
 “veh” = vehicle or “anta” = antalarmin 20 mg/kg p.o.) “treat” = treatment
 received (either “sal” = saline or “mor” = morphine 2.5 mg/kg i.p.) “P”
 stands for “Phase”, “S” stands for “Side” “P1_S1” = time spent (sec) on
 the mouse half-chamber during the habituation phase (empty) “P1_S2” = time
 spent (sec) on the object half-chamber during the habituation phase
 (empty) “P2_S1” = time spent (sec) on the mouse half-chamber during the
 sociability phase “P2_S2” = time spent (sec) on the object half-chamber
 during the sociability phase “soc ratio” = % of time spent with the mouse,
 calculated as [P2_S1/(P2_S1+P2_S2)]*100 “loco hab” = distance travelled
 (meters) by the subject mouse during the habituation phase “loco soc” =
 distance travelled (meters) by the subject mouse during the sociability
 phase In red and bold are noted the mean and the SEM of the data in the
 corresponding column **Table 3 – 3CH CRF1 KO males** This tab contains the
 behavioral data reported in **Figure 3B** and **Figure 3 – figure
 supplement 1** “3CH” stands for “three-chamber”, “CRF1” stands for “CRF1
 mice“ “subject” = ID number of the subject animal “geno” = genotype
 (either “WT” = wild-type, “HET” = heterozygous, or “KO” = knock-out)
 “treat” = treatment received (either “SAL” = saline or “MOR” = morphine
 0.625 mg/kg i.p.) “P” stands for “Phase”, “S” stands for “Side” “P1_S1” =
 time spent (sec) on the mouse half-chamber during the habituation phase
 (empty) “P1_S2” = time spent (sec) on the object half-chamber during the
 habituation phase (empty) “P2_S1” = time spent (sec) on the mouse
 half-chamber during the sociability phase “P2_S2” = time spent (sec) on
 the object half-chamber during the sociability phase “soc ratio” = % of
 time spent with the mouse, calculated as [P2_S1/(P2_S1+P2_S2)]*100 “loco
 hab” = distance travelled (meters) by the subject mouse during the
 habituation phase “loco soc” = distance travelled (meters) by the subject
 mouse during the sociability phase In red and bold are noted the mean and
 the SEM of the data in the corresponding column **Table 4 – Ephy C57
 males** This tab contains the electrophysiological data reported in
 **Figure 2B** and **Figure 4C-D** “Ephy” stands for “electrophysiology”,
 “C57” stands for “C57BL/6“ “veh” stands for “vehicle”, “anta” stands for
 “antalarmin” (20 mg/kg p.o.) “sal” stands for “saline”, “mor” stands for
 “morphine” (2.5 mg/kg i.p.) “ALL CELLS” corresponds to the data in Figure
 2B “OXY-AVP” corresponds to the data in Figure 4C “AVP” corresponds to the
 data in Figure 4D “OXY” stands for “oxytocin”, “AVP” stands for
 “vasopressin” In red and bold are noted the mean and the SEM of the data
 in the corresponding column **Table 5 – Ephy C57 females** This tab
 contains the electrophysiological data reported in **Figure 2D** and
 **Figure 4F-G** “Ephy” stands for “electrophysiology”, “C57” stands for
 “C57BL/6“ “veh” stands for “vehicle”, “anta” stands for “antalarmin” (20
 mg/kg p.o.) “sal” stands for “saline”, “mor” stands for “morphine” (2.5
 mg/kg i.p.) “ALL CELLS” corresponds to the data in Figure 2D “OXY-AVP”
 corresponds to the data in Figure 4F “OXY” highlighted in yellow
 corresponds to the data in Figure 4G “OXY” stands for “oxytocin”, “AVP”
 stands for “vasopressin” In red and bold are noted the mean and the SEM of
 the data in the corresponding column **Table 6 – Ephy CRF1 KO males** This
 tab contains the electrophysiological data reported in **Figure 3E**
 “Ephy” stands for “electrophysiology”, “CRF1” stands for “CRF1 mice“ “wt”
 = wild-type, “het” = heterozygous, or “ko” = knock-out “sal” = “saline”,
 “mor” = “morphine” (0.625 mg/kg i.p.) “ALL CELLS” corresponds to the data
 in Figure 3E In red and bold are noted the mean and the SEM of the data in
 the corresponding column