cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10026353
title: Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens

description: ABSTRACT: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM.
DOI: 10.6084/m9.figshare.10026353.v1
title: Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens

description: ABSTRACT: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM.
DOI: 10.6084/m9.figshare.10073222
title: Recovery of chitin and chitosan from shrimp waste with microwave technique and versatile application

description: ABSTRACT: In this study, chitin and chitosan were extracted from Litopenaeus vannamei waste using chemical and microwave methods. Shrimp waste was cleaned, dried and ground sieved to 16, 32 and 60 mesh, and the samples were depigmented, demineralized, and deproteinized. Then, the chitin was submitted to a deacetylation process by 45% NaOH solution under microwave irradiation at 600w, for intermittent 15 min or using 5 pulses of 5 minutes. The study showed that the effectiveness of the particle size of 32 mesh and 6 pulses of 5 min to deacetylation with 92% of degree and chitosan yield (52.2%). The polymer chitosan showed higher antimicrobial activity against to Staphylococcus aureus, Escherichia coli, Salmonella enterica and the yeast Candida sp., respectively. The results indicated the feasibility of the microwave radiation as an attractive method to recover chitin and chitosan from shrimp wastes.
DOI: 10.6084/m9.figshare.10074152
title: Isolation of Salmonella Typhimurium, Listeria monocytogenes and coagulase-positive Staphylococcus from salami sold at street fairs in Porto Alegre, Brazil

description: ABSTRACT: Salami is a ready-to-eat (RTE) product frequently purchased at street fairs in Porto Alegre. Salmonella enterica, Listeria monocytogenes, and coagulase-positive Staphylococcus (CPS) are important causes of foodborne disease and can be transmitted through the consumption of RTE foods. The aim of this study was to evaluate the presence of these pathogens in salami sold at street fairs. Ninety salami samples from three commercial brands available at street fairs were analyzed by routine bacteriological methods for Salmonella spp. and Listeria spp., as well as enumeration of CPS. In addition, two samples from each commercial brand were analyzed for water activity (aw). Samples of brand A showed aw values (0.938 and 0.942) above those set by the legislation, while brand B (0.849 and 0.860) and brand C (0.826 and 0.854) were compliant. Microbiological analyses showed that 67.7% were negative to all investigated bacteria. Salmonella Typhimurium was isolated from 4.4% (4/90) of salami samples, all from commercial brand A. Listeria monocytogenes was detected in 3.3% (3/90) of samples, from commercial brands B and C. Moreover, 7.7% (7/90) of samples contained CPS populations non-compliant with legislation. Although the great majority of salami sold at street fairs of Porto Alegre was compliant with standards, S. enterica, L. monocytogenes, and CPS ≥ 5 × 103 cfu.g-1 could be found in this RTE product. Therefore, control measures in the processing industry and consumer’s education about foodborne illness prevention should be maintained.
DOI: 10.6084/m9.figshare.10074152.v1
title: Isolation of Salmonella Typhimurium, Listeria monocytogenes and coagulase-positive Staphylococcus from salami sold at street fairs in Porto Alegre, Brazil

description: ABSTRACT: Salami is a ready-to-eat (RTE) product frequently purchased at street fairs in Porto Alegre. Salmonella enterica, Listeria monocytogenes, and coagulase-positive Staphylococcus (CPS) are important causes of foodborne disease and can be transmitted through the consumption of RTE foods. The aim of this study was to evaluate the presence of these pathogens in salami sold at street fairs. Ninety salami samples from three commercial brands available at street fairs were analyzed by routine bacteriological methods for Salmonella spp. and Listeria spp., as well as enumeration of CPS. In addition, two samples from each commercial brand were analyzed for water activity (aw). Samples of brand A showed aw values (0.938 and 0.942) above those set by the legislation, while brand B (0.849 and 0.860) and brand C (0.826 and 0.854) were compliant. Microbiological analyses showed that 67.7% were negative to all investigated bacteria. Salmonella Typhimurium was isolated from 4.4% (4/90) of salami samples, all from commercial brand A. Listeria monocytogenes was detected in 3.3% (3/90) of samples, from commercial brands B and C. Moreover, 7.7% (7/90) of samples contained CPS populations non-compliant with legislation. Although the great majority of salami sold at street fairs of Porto Alegre was compliant with standards, S. enterica, L. monocytogenes, and CPS ≥ 5 × 103 cfu.g-1 could be found in this RTE product. Therefore, control measures in the processing industry and consumer’s education about foodborne illness prevention should be maintained.
DOI: 10.6084/m9.figshare.10073222.v1
title: Recovery of chitin and chitosan from shrimp waste with microwave technique and versatile application

description: ABSTRACT: In this study, chitin and chitosan were extracted from Litopenaeus vannamei waste using chemical and microwave methods. Shrimp waste was cleaned, dried and ground sieved to 16, 32 and 60 mesh, and the samples were depigmented, demineralized, and deproteinized. Then, the chitin was submitted to a deacetylation process by 45% NaOH solution under microwave irradiation at 600w, for intermittent 15 min or using 5 pulses of 5 minutes. The study showed that the effectiveness of the particle size of 32 mesh and 6 pulses of 5 min to deacetylation with 92% of degree and chitosan yield (52.2%). The polymer chitosan showed higher antimicrobial activity against to Staphylococcus aureus, Escherichia coli, Salmonella enterica and the yeast Candida sp., respectively. The results indicated the feasibility of the microwave radiation as an attractive method to recover chitin and chitosan from shrimp wastes.
DOI: 10.6084/m9.figshare.10438088
title: Entomofauna associated with Avena sativa in southern Mato Grosso do Sul, Brazil

description: ABSTRACT This study was aimed at examining the composition of insects and fluctuations in their populations in Avena sativa in southern Mato Grosso do Sul. The study was carried out at the experimental farm of the Federal University of Grande Dourados (FAECA) in Dourados, Mato Grosso do Sul, in 2014 and 2015, in an area of three hectares divided into 80 plots of 169 m2 each. The assessments were carried out weekly by sampling the area within a 0.25-m2 metal frame, totaling ten evaluations. Within the frame, ten plants were examined for aphids and all plants were inspected for other insects. Based on the faunistic analysis (abundance, constancy, frequency, and dominance), eight species were observed during the two years of study. The most frequent, abundant, dominant, and recurring species were Rhopalosiphum padi, Spodoptera frugiperda and dipterans of the family Syrphidae (hoverfly). The population of R. padi increased until approximately the 40th day after emergence (DAE), when the highest abundance of hoverflies was also observed. After that, the population of R. padi decreased. The aphid R. padi was the main insect observed in A. sativa during the two years of study but were naturally controlled by hoverflies; therefore, chemical intervention was not needed.
DOI: 10.6084/m9.figshare.10438088.v1
title: Entomofauna associated with Avena sativa in southern Mato Grosso do Sul, Brazil

description: ABSTRACT This study was aimed at examining the composition of insects and fluctuations in their populations in Avena sativa in southern Mato Grosso do Sul. The study was carried out at the experimental farm of the Federal University of Grande Dourados (FAECA) in Dourados, Mato Grosso do Sul, in 2014 and 2015, in an area of three hectares divided into 80 plots of 169 m2 each. The assessments were carried out weekly by sampling the area within a 0.25-m2 metal frame, totaling ten evaluations. Within the frame, ten plants were examined for aphids and all plants were inspected for other insects. Based on the faunistic analysis (abundance, constancy, frequency, and dominance), eight species were observed during the two years of study. The most frequent, abundant, dominant, and recurring species were Rhopalosiphum padi, Spodoptera frugiperda and dipterans of the family Syrphidae (hoverfly). The population of R. padi increased until approximately the 40th day after emergence (DAE), when the highest abundance of hoverflies was also observed. After that, the population of R. padi decreased. The aphid R. padi was the main insect observed in A. sativa during the two years of study but were naturally controlled by hoverflies; therefore, chemical intervention was not needed.
DOI: 10.6084/m9.figshare.10438049
title: Feeding preference of Spodoptera frugiperda on different sorghum genotypes

description: ABSTRACT The resistance of plants to pests is a feature of great importance for agriculture, as it reduces costs with insecticides and promotes increased yield resulting in higher profits. This work aimed to evaluate the feeding preference of the Spodoptera frugiperda caterpillar by different sorghum genotypes. The experiment was carried out at the Entomology Laboratory of Universidade Estadual de Mato Grosso do Sul, at University Unit of Cassilândia, from March to June 2016. The experimental design was completely randomized with ten replicates. The treatments were composed by seven sorghum genotypes: Agromen 50A40, Agromen 50A50, DOW 1G100, DOW 1G220, DOW 1G233, XB 6022 and LG 6310. Evaluations were performed with 1st instar caterpillars. The number of caterpillars that settled in the genotypes at 5, 10, 15, 20, 25, 30, 60 minutes and 24 hours after the infestation was recorded. The feeding preference index and the fresh leaf mass consumed were estimated. The Agromen 50A40 genotype showed lower attractiveness rating for the S. frugiperda among all evaluated sorghum genotypes.
DOI: 10.6084/m9.figshare.10438049.v1
title: Feeding preference of Spodoptera frugiperda on different sorghum genotypes

description: ABSTRACT The resistance of plants to pests is a feature of great importance for agriculture, as it reduces costs with insecticides and promotes increased yield resulting in higher profits. This work aimed to evaluate the feeding preference of the Spodoptera frugiperda caterpillar by different sorghum genotypes. The experiment was carried out at the Entomology Laboratory of Universidade Estadual de Mato Grosso do Sul, at University Unit of Cassilândia, from March to June 2016. The experimental design was completely randomized with ten replicates. The treatments were composed by seven sorghum genotypes: Agromen 50A40, Agromen 50A50, DOW 1G100, DOW 1G220, DOW 1G233, XB 6022 and LG 6310. Evaluations were performed with 1st instar caterpillars. The number of caterpillars that settled in the genotypes at 5, 10, 15, 20, 25, 30, 60 minutes and 24 hours after the infestation was recorded. The feeding preference index and the fresh leaf mass consumed were estimated. The Agromen 50A40 genotype showed lower attractiveness rating for the S. frugiperda among all evaluated sorghum genotypes.
DOI: 10.6084/m9.figshare.11266895.v1
title: Essential oils of Tahiti lemon and cinnamon bark in control of storage fungi and the physiological and sanitary quality of beans

description: ABSTRACT: With the objective to evaluate the efficiency of essential oils of Citrus latifolia (Tahiti lemon) and Cinnamomum zeylanicum (cinnamon bark) in the control of plant pathogens Penicillium sp. and Aspergillus sp. and the quality of the bean seeds, two experiments were conducted. In the first one, the effect of essential oils of C. latifolia and C. zeylanicum was evaluated in vitro development of the fungi Penicillium sp. and Aspergillus sp. and, in the second one, the influence of essential oils on the physiological and sanitary quality of bean seeds. The variables mycelial growth, conidial germination and sporulation of Penicillium sp. and Aspergillus sp. were measured in the first experiment, while the seed germination test, first count of germination, germination speed index (GSI) and sanity test of bean seeds were measured in the second. The essential oil (EO) of C. zeylanicum was more efficient than C. latifolia in the control of Aspergillus sp. and Penicillium sp., but decreased the physiological quality of the beans seeds. The fungal diversity identified in the seed health test was composed by fungi of the genera Aspergillus, Penicillium, Cladosporium, Fusarium, Chaetomium and Rhizopus. The results indicate the potential of the use of these EOs in the seeds treatment.
DOI: 10.6084/m9.figshare.11314469
title: Silicon as resistance inducer in to control black aphid Aphis craccivora Koch, 1854 in Phaseolus lunatus lima beans

description: ABSTRACT In Brazil, there are few records of insects associated with the cultivation of lima beans; among them, there is the black aphid Aphis craccivora Koch, 1854. The objective of this study was to evaluate the effects of silicon application on the resistance induction of lima bean plants, Phaseolus lunatus, to the black aphid A. craccivora. The experiment was conducted in the Entomology Laboratory of the Phytosanitary Sector of Centro de Ciências Agrárias, Universidade Federal do Piauí (UFPI), Brazil. The effects of the following treatments on biological aspects of the insect were evaluated: silicon applied to soil; silicon applied to soil + leaf; silicon applied to leaf; and control, without silicon application. The following biological variables were evaluated: generation period, reproductive period, and the fertility and daily average of produced nymphs per female. Plant silicon and lignin content were also evaluated. A 1% solution of silicic acid (2.0 g of product diluted in 200 mL of water) was applied around the plant stem (on soil), 15 days after emergence. Leaf application was performed with a 1-L spray, 5 days after the soil application. The non-preference of A. craccivora on lima beans was also evaluated. The evaluations were performed after 48 and 72 hours of infestation by counting nymphs and adults at each leaf section. Silicon application reduces nymph production, thereby interfering in the biological aspects of A. craccivora. Therefore, it can be used in cowpea pest management programs.
DOI: 10.6084/m9.figshare.11314469.v1
title: Silicon as resistance inducer in to control black aphid Aphis craccivora Koch, 1854 in Phaseolus lunatus lima beans

description: ABSTRACT In Brazil, there are few records of insects associated with the cultivation of lima beans; among them, there is the black aphid Aphis craccivora Koch, 1854. The objective of this study was to evaluate the effects of silicon application on the resistance induction of lima bean plants, Phaseolus lunatus, to the black aphid A. craccivora. The experiment was conducted in the Entomology Laboratory of the Phytosanitary Sector of Centro de Ciências Agrárias, Universidade Federal do Piauí (UFPI), Brazil. The effects of the following treatments on biological aspects of the insect were evaluated: silicon applied to soil; silicon applied to soil + leaf; silicon applied to leaf; and control, without silicon application. The following biological variables were evaluated: generation period, reproductive period, and the fertility and daily average of produced nymphs per female. Plant silicon and lignin content were also evaluated. A 1% solution of silicic acid (2.0 g of product diluted in 200 mL of water) was applied around the plant stem (on soil), 15 days after emergence. Leaf application was performed with a 1-L spray, 5 days after the soil application. The non-preference of A. craccivora on lima beans was also evaluated. The evaluations were performed after 48 and 72 hours of infestation by counting nymphs and adults at each leaf section. Silicon application reduces nymph production, thereby interfering in the biological aspects of A. craccivora. Therefore, it can be used in cowpea pest management programs.
DOI: 10.6084/m9.figshare.11266895
title: Essential oils of Tahiti lemon and cinnamon bark in control of storage fungi and the physiological and sanitary quality of beans

description: ABSTRACT: With the objective to evaluate the efficiency of essential oils of Citrus latifolia (Tahiti lemon) and Cinnamomum zeylanicum (cinnamon bark) in the control of plant pathogens Penicillium sp. and Aspergillus sp. and the quality of the bean seeds, two experiments were conducted. In the first one, the effect of essential oils of C. latifolia and C. zeylanicum was evaluated in vitro development of the fungi Penicillium sp. and Aspergillus sp. and, in the second one, the influence of essential oils on the physiological and sanitary quality of bean seeds. The variables mycelial growth, conidial germination and sporulation of Penicillium sp. and Aspergillus sp. were measured in the first experiment, while the seed germination test, first count of germination, germination speed index (GSI) and sanity test of bean seeds were measured in the second. The essential oil (EO) of C. zeylanicum was more efficient than C. latifolia in the control of Aspergillus sp. and Penicillium sp., but decreased the physiological quality of the beans seeds. The fungal diversity identified in the seed health test was composed by fungi of the genera Aspergillus, Penicillium, Cladosporium, Fusarium, Chaetomium and Rhizopus. The results indicate the potential of the use of these EOs in the seeds treatment.
DOI: 10.6084/m9.figshare.11314511
title: Attractive lures for fruit flies in an organic guava orchard

description: ABSTRACT Fruit flies are the biggest obstacle in guava cultivation, with the monitoring of population a fundamental aspect for their management. The objective of this study was to assess the effectiveness of attractive lures for fruit flies in guava trees, produced in an organic system. McPhail traps were installed with 400 mL of solution, in four repetitions, and distributed in randomized blocks in six treatments: Isca Mosca® (5%); Isca Samaritá Tradicional® (5%); Torula® yeast; Bio Anastrepha® (5%); Ceratrap® and guava juice (50%), with 3 weekly reviews. Data on capture were subjected to analysis of variance, and averages were compared (Tukey 5%). The values of fly trap per day (FTD) were computed. The correlation between flies and meteorological variables were evaluated, as well as the monthly cost of lures. A total of 37,917 individuals from the genus Anastrepha and 122 species of flies Ceratitis capitata were collected, being Anastrepha spp. the main genus of fruit fly in the region. The Ceratrap product proved to be superior, followed by Torula, Isca Mosca, and Bio Anastrepha; Isca Samaritá and guava juice presented the lowest results. The standard trapping of females was like the total. The uniformity of attraction presented a drop in all treatments, and Ceratrap was more constant after seven days. The correlations between meteorological factors and population variation were positive and significant only for precipitation. The most expensive treatments (Ceratrap and Torula) were also the most efficient and selective, and the Ceratrap product required less manpower.
DOI: 10.6084/m9.figshare.11314511.v1
title: Attractive lures for fruit flies in an organic guava orchard

description: ABSTRACT Fruit flies are the biggest obstacle in guava cultivation, with the monitoring of population a fundamental aspect for their management. The objective of this study was to assess the effectiveness of attractive lures for fruit flies in guava trees, produced in an organic system. McPhail traps were installed with 400 mL of solution, in four repetitions, and distributed in randomized blocks in six treatments: Isca Mosca® (5%); Isca Samaritá Tradicional® (5%); Torula® yeast; Bio Anastrepha® (5%); Ceratrap® and guava juice (50%), with 3 weekly reviews. Data on capture were subjected to analysis of variance, and averages were compared (Tukey 5%). The values of fly trap per day (FTD) were computed. The correlation between flies and meteorological variables were evaluated, as well as the monthly cost of lures. A total of 37,917 individuals from the genus Anastrepha and 122 species of flies Ceratitis capitata were collected, being Anastrepha spp. the main genus of fruit fly in the region. The Ceratrap product proved to be superior, followed by Torula, Isca Mosca, and Bio Anastrepha; Isca Samaritá and guava juice presented the lowest results. The standard trapping of females was like the total. The uniformity of attraction presented a drop in all treatments, and Ceratrap was more constant after seven days. The correlations between meteorological factors and population variation were positive and significant only for precipitation. The most expensive treatments (Ceratrap and Torula) were also the most efficient and selective, and the Ceratrap product required less manpower.