DOI: 10.5061/dryad.jsxksn0px
title: Time spares no male: The age-dependent decline of olfactory attractiveness in red flour beetles <em>Tribolium castaneum</em>

description: This dataset accompanies a study investigating how male age affects
 olfactory attractiveness and locomotor activity in the red flour beetle,
 Tribolium castaneum. The Excel file includes four sheets: female responses
 to odors from different males (inter-individual), responses to the same
 males at different ages (intra-individual), and walking activity before
 and after mating. Variables include male age, latency to contact odor
 sources, and walking distance. The dataset supports reuse in behavioral
 ecology, chemical communication, and senescence studies. All data are
 original and free of restrictions under the CC0 waiver.
description: # README: Time spares no male: The age-dependent decline of olfactory
 attractiveness in red flour beetles *Tribolium castaneum* ## Authors
 Hinata Kikuchi¹, Kentarou Matsumura²* 1. Graduate School of Agriculture,
 Kagawa University, Kagawa, Japan 2. Graduate School of Arts and Sciences,
 The University of Tokyo, Komaba, Tokyo, Japan\ *Corresponding author:
 [matsumura@g.ecc.u-tokyo.ac.jp](mailto:matsumura@g.ecc.u-tokyo.ac.jp) ##
 1. Description of the dataset This dataset supports the study on how male
 age affects olfactory attractiveness and locomotor activity in *Tribolium
 castaneum*. It includes behavioral trials measuring female responses to
 male odors and walking activity of males before and after mating. The
 Excel file contains four sheets: 1. **inter-individual** – female response
 to odors from different males (young vs. old) 2. **intra-individual** –
 female response to odors from the same male at different ages 3. **before
 mating walking** – walking activity before mating 4. **after mating
 walking** – walking activity after mating This dataset is useful for reuse
 in studies on sexual selection, insect behavior, olfactory communication,
 and senescence. ## 2. File overview | File name | Description | Format | |
 -------------------------------------- |
 ----------------------------------------------- | ------------- | |
 `data_Kikuchi___Matsumura_(2025).xlsx` | Excel file with four sheets as
 described above. | Excel (.xlsx) | ## 3. Sheet and variable descriptions
 ### Sheet 1: inter-individual * **male_age**: categorical variable
 (“young”, “old”) * **latency_s**: time (in seconds) until female first
 contacts male-scented paper Each row = one female trial comparing scent of
 different males. ### Sheet 2: intra-individual * **male_ID**: ID of each
 male (1–10) * **male_age**: categorical variable (“young”, “old”) *
 **latency_s**: time (in seconds) until female first contacts the scent
 paper Each row = response to the same male at different ages. ### Sheet 3:
 before mating walking * **strain**: “H” (high-activity) or “L”
 (low-activity) strain * **replicate**: “A”, “B”, or “C” * **distance_mm**:
 total walking distance before mating Each row = activity of a male before
 mating. ### Sheet 4: after mating walking * **strain**: “H” or “L” *
 **replicate**: “A”, “B”, or “C” * **distance_mm**: total walking distance
 after mating Each row = activity of a male after mating. ## 4. Software
 and reproducibility Statistical analysis was performed in **R version
 4.1.0** using the **car** package (Fox & Weisberg, 2019). * Female
 odor preference (*latency*) was analyzed using GLMs with gamma error
 distribution. * Intra-individual age effects were analyzed with GLMMs,
 with **male_ID** as a random effect. * Model selection was based on
 **AIC**.\ Reproducible code and plots are included in `analysis_code.R`.
 ## 5. License and usage notes All data in this repository are released
 under the **CC0 1.0 Universal (Public Domain Dedication)** license. Users
 are free to copy, modify, distribute, and use the data without permission
 or attribution. ## 6. Contact information For questions or clarifications,
 please contact:\ **Kentarou Matsumura**\ Graduate School of Arts and
 Sciences, The University of Tokyo\ 📧
 [matsumura@g.ecc.u-tokyo.ac.jp](mailto:matsumura@g.ecc.u-tokyo.ac.jp) ##
 Notes * Units: seconds (latency), millimeters (distance) * No data were
 removed or imputed * Variable names match the R script