cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0hm582d
title: CIL: 52822, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given tile position
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: Tile x=1, y=3, z=20 from PS11_167511L_Myc_Terc
DOI: 10.6075/j0td9x4d
title: CIL: 54421, Drosophila melanogaster, Entire Organism. In Cell Image Library

description: 24.3 degrees ℃
description: Video of fruit fly embryonic development. Video filename format is as follows organism_temperature_embryo. Data was collected at 24.3 degrees Celcius
description: Imaging mode: Gray Scale
Visualization methods: 20x magnification captured by one of the following eyepiece cameras: Swiftcam 3 Megapixel, OMAX 9.0 MP, AmScope MU300
DOI: 10.6075/j04m942v
title: CIL: 52380, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: One z layer of a z-stack at the given tile position
description: Tile x=4, y=2, z=19 from BL024199_Myc_Terc
DOI: 10.6075/j00z72vs
title: CIL: 52943, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given image
description: Image 2, z=15 from SC_011
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
DOI: 10.6075/j01z444c
title: CIL: 54005, Homo sapiens, HeLa. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: STE family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Visualization methods: confocal microscopy
DOI: 10.6075/j0wm1cz6
title: CIL: 52677, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given tile position
description: Tile x=3, y=4, z=1 from PS11_10021_2B_Myc_Terc
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
DOI: 10.6075/j02r3rct
title: CIL: 54028. In Cell Image Library

description: Visualization methods: confocal microscopy
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: STE family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
DOI: 10.6075/j0571bnf
title: CIL: 53299, Procaryot, Bacterium. In Cell Image Library

description: Magnification 3.5,000X
description: Imaging mode: recorded image
Visualization methods: scanning electron microscopy (SEM)
description: MRSA treated 3.5KX
DOI: 10.6075/j0fq9w8g
title: CIL: 53484, Homo sapiens, HeLa. In Cell Image Library

description: AGC family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: Visualization methods: confocal microscopy
DOI: 10.6075/j0ws8sx3
title: CIL: 53497. In Cell Image Library

description: AGC family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Visualization methods: confocal microscopy
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
DOI: 10.6075/j0dv1jd5
title: CIL: 52434, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given tile position
description: Tile x=5, y=3, z=10 from BL024199_Myc_Terc
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
DOI: 10.6075/j0r49qcp
title: CIL: 53006, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: Image 1, z=15 from SC_028
description: One z layer of a z-stack at the given image
DOI: 10.6075/j07944ct
title: CIL: 54052. In Cell Image Library

description: TK family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: Visualization methods: confocal microscopy
DOI: 10.6075/j0dr2v2r
title: CIL: 52642, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: Tile x=2, y=7, z=8 from PS09_287383C_Myc_Terc
description: One z layer of a z-stack at the given tile position
DOI: 10.6075/j0kp81wq
title: CIL: 54027, Homo sapiens, HeLa. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: Visualization methods: confocal microscopy
description: STE family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
DOI: 10.6075/j0251hr7
title: CIL: 52696, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Tile x=3, y=4, z=20 from PS11_10021_2B_Myc_Terc
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: One z layer of a z-stack at the given tile position
DOI: 10.6084/m9.figshare.29148584
title: A Click Chemistry Approach for the Synthesis of Triazole Linked Vanillin Scaffolds as Potent Pharmacophores: Anti-Diabetic, Anti-Inflammatory, Antioxidant, Molecular Docking and ADMET Investigations

description: In this study a series of triazole linked vanillin derivatives were synthesized via Click chemistry and characterized using FTIR,<sup>1</sup>H NMR,<sup>1</sup>³C NMR, and HRMS/MS. Among the synthesized compounds, <b>6k, 6f,</b> and <b>6b</b> showed strong α-amylase inhibitory activity (IC<sub>50</sub> = 50.70, 51.36, and 53.25 μg/mL, respectively) and α-glucosidase inhibitory activity (IC<sub>50</sub> = 36.85, 37.99, and 38.49 μg/mL), comparable to acarbose (IC<sub>50</sub> = 54.38 and 39.04 μg/mL). Compound <b>6e</b> exhibited potent antibacterial activity (MIC = 0.5–2 μg/mL) against all tested strains, including <i>S. aureus</i>, <i>S. pyogenes</i>, <i>S. typhi</i>, and <i>P. aeruginosa</i>, comparable to streptomycin, while <b>6k</b> also showed notable inhibition (MIC = 2–4 μg/mL). In antioxidant assays, <b>6a</b> and <b>6j</b> demonstrated IC<sub>50</sub> values (48.25 and 49.31 μg/mL) comparable to ascorbic acid (IC<sub>50</sub> = 49.18 μg/mL). Anti-inflammatory activity was significant for <b>6a</b> and <b>6j</b> (IC<sub>50</sub> = 36.66 and 37.21 μg/mL), surpassing diclofenac (IC<sub>50</sub> = 37.89 μg/mL). The compounds were nontoxic in cytotoxicity studies, with IC<sub>50</sub> values &gt;120 μg/mL for Vero cells, significantly higher than cisplatin (35.15 μg/mL). Molecular docking supported the <i>in vitro</i> results, with active compounds showing strong binding affinities, and SwissADME analysis indicated favorable pharmacokinetic properties. These findings highlight the multifunctional therapeutic potential of the designed compounds as anti-diabetic, antibacterial, antioxidant, and anti-inflammatory agents.
DOI: 10.6084/m9.figshare.29148584.v1
title: A Click Chemistry Approach for the Synthesis of Triazole Linked Vanillin Scaffolds as Potent Pharmacophores: Anti-Diabetic, Anti-Inflammatory, Antioxidant, Molecular Docking and ADMET Investigations

description: In this study a series of triazole linked vanillin derivatives were synthesized via Click chemistry and characterized using FTIR,<sup>1</sup>H NMR,<sup>1</sup>³C NMR, and HRMS/MS. Among the synthesized compounds, <b>6k, 6f,</b> and <b>6b</b> showed strong α-amylase inhibitory activity (IC<sub>50</sub> = 50.70, 51.36, and 53.25 μg/mL, respectively) and α-glucosidase inhibitory activity (IC<sub>50</sub> = 36.85, 37.99, and 38.49 μg/mL), comparable to acarbose (IC<sub>50</sub> = 54.38 and 39.04 μg/mL). Compound <b>6e</b> exhibited potent antibacterial activity (MIC = 0.5–2 μg/mL) against all tested strains, including <i>S. aureus</i>, <i>S. pyogenes</i>, <i>S. typhi</i>, and <i>P. aeruginosa</i>, comparable to streptomycin, while <b>6k</b> also showed notable inhibition (MIC = 2–4 μg/mL). In antioxidant assays, <b>6a</b> and <b>6j</b> demonstrated IC<sub>50</sub> values (48.25 and 49.31 μg/mL) comparable to ascorbic acid (IC<sub>50</sub> = 49.18 μg/mL). Anti-inflammatory activity was significant for <b>6a</b> and <b>6j</b> (IC<sub>50</sub> = 36.66 and 37.21 μg/mL), surpassing diclofenac (IC<sub>50</sub> = 37.89 μg/mL). The compounds were nontoxic in cytotoxicity studies, with IC<sub>50</sub> values &gt;120 μg/mL for Vero cells, significantly higher than cisplatin (35.15 μg/mL). Molecular docking supported the <i>in vitro</i> results, with active compounds showing strong binding affinities, and SwissADME analysis indicated favorable pharmacokinetic properties. These findings highlight the multifunctional therapeutic potential of the designed compounds as anti-diabetic, antibacterial, antioxidant, and anti-inflammatory agents.
DOI: 10.6075/j0hd7v7c
title: CIL: 53064, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: One z layer of a z-stack at the given image
description: Image 4, z=10 from SC_028
DOI: 10.6075/j0jh3kpq
title: CIL: 52246, Mus musculus, Retinal Pigment Epithelial cells. In Cell Image Library

description: Mouse eye was enucleated, fixed for 10 min in 10% neutral buffered formalin, and washed three times with PBS. Flatmounting was done by making four radial cuts from the center of the cornea back toward the optic nerve. The iris and retina were removed using forceps and additional incisions were made at the ciliary body/cornea margin to relieve tension from the sclera. The RPE flatmounts were stained for rabbit anti-ZO1 antibody (1:100 dilution, catalog no. 61-7300; Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG secondary antibody (catalog no. O11038; Invitrogen). Imaging of the flatmounts was performed with a confocal imaging system (model C1; Nikon) using argon laser excitation at 488 nm. Confocal images were digitally merged (Adobe Photoshop CS2; Adobe, San Jose, CA).
description: Merged confocal image of mouse (C57BL/6J, postnatal day 720) retinal pigment epithelium (RPE) flatmount. The image is stained for ZO-1 (Green, tight junctions between cells) and nucleus (Red, Propidium Iodide). The RPE cells are in general regularly shaped and arranged polygonal cells, however, their shape and arrangement changes significantly according to their location and age.
description: Imaging mode: confocal microscopy
Visualization methods: primary antibody plus labeled secondary antibody
DOI: 10.6084/m9.figshare.29185589
title: Probing and Evaluating the Anion Binding Studies in Novel Coumarin Based Strapped Calix[4]Pyrrole

description: A novel coumarin-based strapped calix[4]pyrrole, C4P9, was synthesized through an acid cyclization reaction followed by the reaction of coumarin with dipyrromethane. The structure of <b>C4P9</b> was confirmed using <sup>1</sup>H-NMR,<sup>13</sup>C-NMR, and mass spectrometry. Structural analysis revealed a pre-organized conformation for anion recognition, facilitated by intramolecular hydrogen bonding and the rigid strapping unit. An in-depth investigation of anion binding with various species such as F<sup>−</sup>, Br<sup>−</sup>, Cl<sup>−</sup>, I<sup>−</sup>, AcO<sup>−</sup>, NO<sub>3</sub><sup>−</sup>, HSO<sub>4</sub><sup>−</sup>, SCN<sup>−</sup>, and H<sub>2</sub>PO<sub>4</sub><sup>−</sup> was conducted using UV-vis and NMR spectroscopy. The anion binding studies were validated by comparing <sup>1</sup>H-NMR spectra of free receptor <b>C4P9</b> with that of <b>C4P9@F</b>. The results from the online supramolecular Bindfit v0.5 program and Job’s plots indicated a 1:1 stoichiometry for the complexation between <b>C4P9</b> and anions. The coumarin strap enhanced the binding ability and selectivity toward anions, as evidenced by calculations of binding constants. This was observed through interactions such as anion—π (<i>involving aromatic moieties at the meso-position</i>) and C–H—anion (<i>involving the strapped linker and aromatic moiety</i>) as well as N–H—anion interactions. This study demonstrates the potential of coumarin-based strapped calix[4]pyrrole as a versatile platform for developing functional anion receptors, particularly in anion/ion-pair coordination chemistry and supramolecular chemistry in general.
DOI: 10.6084/m9.figshare.29185589.v1
title: Probing and Evaluating the Anion Binding Studies in Novel Coumarin Based Strapped Calix[4]Pyrrole

description: A novel coumarin-based strapped calix[4]pyrrole, C4P9, was synthesized through an acid cyclization reaction followed by the reaction of coumarin with dipyrromethane. The structure of <b>C4P9</b> was confirmed using <sup>1</sup>H-NMR,<sup>13</sup>C-NMR, and mass spectrometry. Structural analysis revealed a pre-organized conformation for anion recognition, facilitated by intramolecular hydrogen bonding and the rigid strapping unit. An in-depth investigation of anion binding with various species such as F<sup>−</sup>, Br<sup>−</sup>, Cl<sup>−</sup>, I<sup>−</sup>, AcO<sup>−</sup>, NO<sub>3</sub><sup>−</sup>, HSO<sub>4</sub><sup>−</sup>, SCN<sup>−</sup>, and H<sub>2</sub>PO<sub>4</sub><sup>−</sup> was conducted using UV-vis and NMR spectroscopy. The anion binding studies were validated by comparing <sup>1</sup>H-NMR spectra of free receptor <b>C4P9</b> with that of <b>C4P9@F</b>. The results from the online supramolecular Bindfit v0.5 program and Job’s plots indicated a 1:1 stoichiometry for the complexation between <b>C4P9</b> and anions. The coumarin strap enhanced the binding ability and selectivity toward anions, as evidenced by calculations of binding constants. This was observed through interactions such as anion—π (<i>involving aromatic moieties at the meso-position</i>) and C–H—anion (<i>involving the strapped linker and aromatic moiety</i>) as well as N–H—anion interactions. This study demonstrates the potential of coumarin-based strapped calix[4]pyrrole as a versatile platform for developing functional anion receptors, particularly in anion/ion-pair coordination chemistry and supramolecular chemistry in general.