cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.29822196
title: Trichostatin A ameliorated combined radiation and skin wound injury-induced mortality and hematopoietic suppression in a rat model

description: Effective countermeasure modalities against combined radiation injury, where the consequences of radiation exposure are highly complex with one or more conditions like burns, wounds, or infection, are yet far from reality. The current study aims to evaluate Trichostatin A (TSA), a presumptive radiomitigative molecule, in a rat model of combined radiation and wound injury (CRWI). Sprague-Dawley rats (200 ± 10 g) were whole-body exposed to different doses of <sup>60</sup>Co-γ-irradiation (4–7Gy) alone and with an excision wound (EW, size 20 ± 2 mm diameter), to optimize CRWI radiation dose. The efficacy of TSA (75 ng/kg b.w.) was evaluated at LD<sub>50/30</sub> CRWI dose, by administering intravenously in 2- and 5-doses, starting at 1 h post-irradiation and thereafter every 24 h to monitor 30-day survival, wound area, body weight, bacterial load, systemic changes, and histology. CRWI radiation dose-response curve rendered 5 Gy as LD<sub>50/30</sub>. Dose-dependent study of TSA showed that 5-doses and 2-doses rendered 83.3% and 50% survival respectively, against 50% survival after CRWI (5 Gy + EW). Treatment with TSA-5-doses resulted in 99% wound reduction (vs. 83% in CRWI); recovered CRWI-induced body weight loss, WBC, and platelets; ameliorated bacterial load in blood and skin wound; downregulated apoptosis (Bax expression) and cytotoxicity in bone marrow cells; reduced inflammation (TNF-α, IL-6, IL-1β), and normalized systemic histone deacetylase along-with restoration of structural changes in skin, bone marrow, and spleen. This study demonstrated that TSA substantially enhanced the survival of animals exposed to CRWI probably by ameliorating hematopoietic suppression and delayed wound healing. The therapeutic potential of Trichostatin A (TSA), a histone deacetylase inhibitor, in improving survival in combined radiation and wound injury (CRWI) rat model was studied. Exposure to radiation coupled with wound injury leads to severe outcomes characterized by impaired hematopoiesis, systemic inflammation, and delayed wound healing. TSA treatment significantly improved survival by mitigating hematopoietic suppression with the restoration of white blood cells, platelets, bone marrow cells, and splenocytes along with the systemic inflammation; restored the structural changes in bone marrow and spleen and normalized the CRWI-induced increase in HDAC activity. Furthermore, TSA accelerated wound healing by restoring structural changes in the skin and minimizing neutrophil infiltration.
DOI: 10.6084/m9.figshare.29822196.v1
title: Trichostatin A ameliorated combined radiation and skin wound injury-induced mortality and hematopoietic suppression in a rat model

description: Effective countermeasure modalities against combined radiation injury, where the consequences of radiation exposure are highly complex with one or more conditions like burns, wounds, or infection, are yet far from reality. The current study aims to evaluate Trichostatin A (TSA), a presumptive radiomitigative molecule, in a rat model of combined radiation and wound injury (CRWI). Sprague-Dawley rats (200 ± 10 g) were whole-body exposed to different doses of <sup>60</sup>Co-γ-irradiation (4–7Gy) alone and with an excision wound (EW, size 20 ± 2 mm diameter), to optimize CRWI radiation dose. The efficacy of TSA (75 ng/kg b.w.) was evaluated at LD<sub>50/30</sub> CRWI dose, by administering intravenously in 2- and 5-doses, starting at 1 h post-irradiation and thereafter every 24 h to monitor 30-day survival, wound area, body weight, bacterial load, systemic changes, and histology. CRWI radiation dose-response curve rendered 5 Gy as LD<sub>50/30</sub>. Dose-dependent study of TSA showed that 5-doses and 2-doses rendered 83.3% and 50% survival respectively, against 50% survival after CRWI (5 Gy + EW). Treatment with TSA-5-doses resulted in 99% wound reduction (vs. 83% in CRWI); recovered CRWI-induced body weight loss, WBC, and platelets; ameliorated bacterial load in blood and skin wound; downregulated apoptosis (Bax expression) and cytotoxicity in bone marrow cells; reduced inflammation (TNF-α, IL-6, IL-1β), and normalized systemic histone deacetylase along-with restoration of structural changes in skin, bone marrow, and spleen. This study demonstrated that TSA substantially enhanced the survival of animals exposed to CRWI probably by ameliorating hematopoietic suppression and delayed wound healing. The therapeutic potential of Trichostatin A (TSA), a histone deacetylase inhibitor, in improving survival in combined radiation and wound injury (CRWI) rat model was studied. Exposure to radiation coupled with wound injury leads to severe outcomes characterized by impaired hematopoiesis, systemic inflammation, and delayed wound healing. TSA treatment significantly improved survival by mitigating hematopoietic suppression with the restoration of white blood cells, platelets, bone marrow cells, and splenocytes along with the systemic inflammation; restored the structural changes in bone marrow and spleen and normalized the CRWI-induced increase in HDAC activity. Furthermore, TSA accelerated wound healing by restoring structural changes in the skin and minimizing neutrophil infiltration.
DOI: 10.6084/m9.figshare.30178575
title: Optimized combination methods for exploring novel space environment-responsive genes and their roles: insights from space-flown <i>C. elegans</i> and their implications for astronauts

description: By expanding the catalog of spaceflight-induced molecular signatures in <i>Caenorhabditis elegans</i>, we aim to identify key molecular features and potential mechanisms underlying space environment-induced health risks to astronauts using <i>C. elegans</i> as a model organism. We employed an optimized combination algorithm that integrated two co-expression network analysis methods and four machine learning-based models to identify space environment-responsive genes (SEGs) in space-flown <i>C. elegans</i>. The expression levels and associated biological processes of human orthologues of identified <i>C. elegans</i> genes were further analyzed using data from the JAXA CFE and NASA Twins studies. A total of 114 SEGs that were implicated in four biological processes, including DNA repair, metabolic process, reproductive and developmental process, and lifespan regulation in space-flown <i>C. elegans</i>. We obtained 19 SEGs as potential indicators associated with health risks of the space environment. Further, the human orthologues of <i>C. elegans</i> SEGs that also exhibited altered expression in the blood samples of astronauts. This study provides new insights into the molecular mechanisms behind spaceflight-induced health risks and highlights potential mechanistic targets for preventive measures. The findings suggest a conserved genetic response to space conditions between <i>C. elegans</i> and astronauts, offering valuable targets for mitigating the health risks of space exploration.
DOI: 10.5517/cc12cm9c
title: CCDC 994272: Experimental Crystal Structure Determination

description: Related Article: M.A. Mansoor, N.M. Huang, V. McKee, T.A. Nirmal Peiris, K.G.U. Wijayantha, Z. Arifin, M. Misran, M. Mazhar|2015|Sol.Energy Mater.Sol.Cells|137|258|doi:10.1016/j.solmat.2015.02.006
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cmbd
title: CCDC 994273: Experimental Crystal Structure Determination

description: Related Article: Artem O. Surov, Katarzyna A. Solanko, Andrew D. Bond, Annette Bauer-Brandl, German L. Perlovich|2014|CrystEngComm|16|6603|doi:10.1039/C4CE00756E
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.6084/m9.figshare.29847515
title: Quality of radiation shapes survival, invasiveness, and migration in ovarian cancer cell lines with different molecular profiles and varying alpha/beta ratios: an in vitro study on behalf of the Multicenter Italian Trials in Ovarian Cancer (MITO) group

description: The results of radiotherapy (RT) in oligometastatic ovarian cancers (OCs) lead to the query whether it is possible to stratify patients based on tumor hallmarks to ensure the best-personalized RT treatment. To address this question, we designed a preclinical study to evaluate the effects of high and low linear energy transfer (LET) radiation while considering molecular features and alpha/beta ratios of different OC cell lines. Exponentially growing human OVSAHO, OVCAR8, COV362, and OVCAR3 cells cultured in T-25 and T-75 flasks were exposed to different single physical doses of photons, protons, and carbon ion (CIRT) irradiation. We assessed ovarian cells’ in vitro response using clonogenic survival (fitted using LQ model), migration by Boyden chamber assay, and invasion through BioCoat Matrigel invasion assay. Following photon irradiation, OVCAR3 was the most radioresistant and OVCAR8 the most radiosensitive cell line. OC cell migration decreased in a dose-dependent manner after irradiation, with CIRT showing the strongest effect, evident by the α/β ratio. The number of invading cells decreased following irradiation with all types. However, the greatest reduction was seen in CIRT, particularly at higher α/β ratios. Proton irradiation demonstrated similar potential to photons but did not match the effects of carbon ions in terms of survival, migration, and invasion<b>. Conclusion:</b> CIRT markedly reduced survival, migration, and invasion of OC cells, particularly in BRCA wild-type OVCAR3 emphasizing its potential to improve local control and lower metastasis risk. This preliminary study serves as a foundation for developing personalized clinical radiation strategies to treat oligometastatic OCs.
DOI: 10.6084/m9.figshare.29847515.v1
title: Quality of radiation shapes survival, invasiveness, and migration in ovarian cancer cell lines with different molecular profiles and varying alpha/beta ratios: an in vitro study on behalf of the Multicenter Italian Trials in Ovarian Cancer (MITO) group

description: The results of radiotherapy (RT) in oligometastatic ovarian cancers (OCs) lead to the query whether it is possible to stratify patients based on tumor hallmarks to ensure the best-personalized RT treatment. To address this question, we designed a preclinical study to evaluate the effects of high and low linear energy transfer (LET) radiation while considering molecular features and alpha/beta ratios of different OC cell lines. Exponentially growing human OVSAHO, OVCAR8, COV362, and OVCAR3 cells cultured in T-25 and T-75 flasks were exposed to different single physical doses of photons, protons, and carbon ion (CIRT) irradiation. We assessed ovarian cells’ in vitro response using clonogenic survival (fitted using LQ model), migration by Boyden chamber assay, and invasion through BioCoat Matrigel invasion assay. Following photon irradiation, OVCAR3 was the most radioresistant and OVCAR8 the most radiosensitive cell line. OC cell migration decreased in a dose-dependent manner after irradiation, with CIRT showing the strongest effect, evident by the α/β ratio. The number of invading cells decreased following irradiation with all types. However, the greatest reduction was seen in CIRT, particularly at higher α/β ratios. Proton irradiation demonstrated similar potential to photons but did not match the effects of carbon ions in terms of survival, migration, and invasion<b>. Conclusion:</b> CIRT markedly reduced survival, migration, and invasion of OC cells, particularly in BRCA wild-type OVCAR3 emphasizing its potential to improve local control and lower metastasis risk. This preliminary study serves as a foundation for developing personalized clinical radiation strategies to treat oligometastatic OCs.
DOI: 10.6084/m9.figshare.30178575.v1
title: Optimized combination methods for exploring novel space environment-responsive genes and their roles: insights from space-flown <i>C. elegans</i> and their implications for astronauts

description: By expanding the catalog of spaceflight-induced molecular signatures in <i>Caenorhabditis elegans</i>, we aim to identify key molecular features and potential mechanisms underlying space environment-induced health risks to astronauts using <i>C. elegans</i> as a model organism. We employed an optimized combination algorithm that integrated two co-expression network analysis methods and four machine learning-based models to identify space environment-responsive genes (SEGs) in space-flown <i>C. elegans</i>. The expression levels and associated biological processes of human orthologues of identified <i>C. elegans</i> genes were further analyzed using data from the JAXA CFE and NASA Twins studies. A total of 114 SEGs that were implicated in four biological processes, including DNA repair, metabolic process, reproductive and developmental process, and lifespan regulation in space-flown <i>C. elegans</i>. We obtained 19 SEGs as potential indicators associated with health risks of the space environment. Further, the human orthologues of <i>C. elegans</i> SEGs that also exhibited altered expression in the blood samples of astronauts. This study provides new insights into the molecular mechanisms behind spaceflight-induced health risks and highlights potential mechanistic targets for preventive measures. The findings suggest a conserved genetic response to space conditions between <i>C. elegans</i> and astronauts, offering valuable targets for mitigating the health risks of space exploration.
DOI: 10.5517/cc12cmcf
title: CCDC 994274: Experimental Crystal Structure Determination

description: Related Article: Artem O. Surov, Katarzyna A. Solanko, Andrew D. Bond, Annette Bauer-Brandl, German L. Perlovich|2014|CrystEngComm|16|6603|doi:10.1039/C4CE00756E
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cmdg
title: CCDC 994275: Experimental Crystal Structure Determination

description: Related Article: Artem O. Surov, Katarzyna A. Solanko, Andrew D. Bond, Annette Bauer-Brandl, German L. Perlovich|2014|CrystEngComm|16|6603|doi:10.1039/C4CE00756E
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccv4v60
title: CCDC 779222: Experimental Crystal Structure Determination

description: Related Article: Zhiyue Han, Zhihua Zhao, Zhiming Du, Chunlin He, Linshuang Zhao, Xiaomin Cong|2014|RSC Advances|4|33724|doi:10.1039/C4RA04999C
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cmqs
title: CCDC 994285: Experimental Crystal Structure Determination

description: Related Article: Ki Hyun Kim, Timothy R. Ramadhar, Christine Beemelmanns, Shugeng Cao, Michael Poulsen, Cameron R. Currie, Jon Clardy|2014|Chemical Science|5|4333|doi:10.1039/C4SC01136H
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc12cmjl
title: CCDC 994279: Experimental Crystal Structure Determination

description: Related Article: Yury N. Kotovshchikov,   Gennadij V. Latyshev,   Nikolay V. Lukashev,  Irina P. Beletskaya|2014|Org.Biomol.Chem.|12|3707|doi:10.1039/C4OB00404C
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc10mmbl
title: CCDC 942379: Experimental Crystal Structure Determination

description: Related Article: Pablo Martín-Ramos, Joana T. Coutinho, Manuela Ramos Silva, Laura C. G. Pereira, Ana M. Matos Beja, Jesus Martin-Gil|2014|J.Chem.Cryst.|44|255|doi:10.1007/s10870-014-0508-4
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc10mmcm
title: CCDC 942380: Experimental Crystal Structure Determination

description: Related Article: Pablo Martín-Ramos, Joana T. Coutinho, Manuela Ramos Silva, Laura C. G. Pereira, Ana M. Matos Beja, Jesus Martin-Gil|2014|J.Chem.Cryst.|44|255|doi:10.1007/s10870-014-0508-4
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccwyghr
title: CCDC 832675: Experimental Crystal Structure Determination

description: Related Article: Shibashis Halder, Sudipto Dey, Corrado Rizzoli and Partha Roy|2014|Polyhedron|78|85|doi:10.1016/j.poly.2014.04.028
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccwygkt
title: CCDC 832677: Experimental Crystal Structure Determination

description: Related Article: Shibashis Halder, Sudipto Dey, Corrado Rizzoli and Partha Roy|2014|Polyhedron|78|85|doi:10.1016/j.poly.2014.04.028
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccwyglv
title: CCDC 832678: Experimental Crystal Structure Determination

description: Related Article: Shibashis Halder, Sudipto Dey, Corrado Rizzoli and Partha Roy|2014|Polyhedron|78|85|doi:10.1016/j.poly.2014.04.028
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccwygmw
title: CCDC 832679: Experimental Crystal Structure Determination

description: Related Article: Shibashis Halder, Sudipto Dey, Corrado Rizzoli and Partha Roy|2014|Polyhedron|78|85|doi:10.1016/j.poly.2014.04.028
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cmy0
title: CCDC 994292: Experimental Crystal Structure Determination

description: Related Article: A.C. Dhieb, D.E. Janzen, M. Rzaigui, W. Smirani Sta|2014|Acta Crystallogr.,Sect.E:Struct.Rep.Online|70|m166|doi:10.1107/S1600536814006989
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cmz1
title: CCDC 994293: Experimental Crystal Structure Determination

description: Related Article: Daniel J. Graham, Shao-Liang Zheng, Daniel G. Nocera|2014|ChemSusChem|7|2449|doi:10.1002/cssc.201402242
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cnmq
title: CCDC 994313: Experimental Crystal Structure Determination

description: Related Article: Christer B. Aakeröy, Tharanga K. Wijethunga, Mohammad Abul Haj, John Desper, Curtis Moore|2014|CrystEngComm|16|7218|doi:10.1039/C4CE00803K
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc12cnnr
title: CCDC 994314: Experimental Crystal Structure Determination

description: Related Article: Christer B. Aakeröy, Tharanga K. Wijethunga, Mohammad Abul Haj, John Desper, Curtis Moore|2014|CrystEngComm|16|7218|doi:10.1039/C4CE00803K
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.