cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/2wben-mv462
title: Crescentin overexpression #1430

description: Raw data files of Crescentin overexpression #1430
description: <strong>Tilt Series Date:</strong> 2006-05-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christine Jacobs Wagner</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2006-05-04-2/rawdata/</p>06apr20b.mrc,  Tilt Series (Pixel Size 0.67 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2006-05-04-2/rawdata/06apr20b.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jcx18-0pw30
title: Crescentin overexpression #1430

description: Raw data files of Crescentin overexpression #1430
description: <strong>Tilt Series Date:</strong> 2006-05-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christine Jacobs Wagner</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2006-05-04-4/rawdata/</p>06apr20b.mrc,  Tilt Series (Pixel Size 0.67 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2006-05-04-4/rawdata/06apr20b.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.zxhw9c
title: Occurrence Download

description: A dataset containing 78488 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Ajuga pyramidalis"
 ]
}

 The dataset includes 78488 records from 368 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002615-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/r1gz4-ssh58
title: E. coli proteinase K

description: Cells were digested with proteinase K after OM was removed
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-29-4/rawdata/</p>ecoliProtK0004.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-29-4/rawdata/ecoliProtK0004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w22w7-mdf57
title: Sporanaerobacter acetigenes

description: Raw data files of Sporanaerobacter acetigenes
description: <strong>Tilt Series Date:</strong> 2012-05-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Sporanaerobacter acetigenes</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-11-5/rawdata/</p>Sacetigenes_manual0005.mrc,  Tilt Series (Pixel Size 1.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-11-5/rawdata/Sacetigenes_manual0005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s2xyr-n4j46
title: fixed Listeria

description: Raw data files of fixed Listeria
description: <strong>Tilt Series Date:</strong> 2008-06-25</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Listeria monocytogenes</p>
<strong>Strain:</strong> 10403S</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Julie Theriot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-06-25-11/rawdata/</p>LIsteria_Leginon_0626_8.mrc,  Tilt Series (Pixel Size 0.961 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-06-25-11/rawdata/LIsteria_Leginon_0626_8.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e88q6-v9y24
title: Agrobacterium

description: Agrobacterium, storage granule, membrane invaginations
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Sample Preparation:</strong> R2/2</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-28-2/rawdata/</p>agroq0003.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-28-2/rawdata/agroq0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vd05h-67c14
title: Haloarcula argentinensis

description: Haloarcula argentinensis
description: <strong>Tilt Series Date:</strong> 2014-06-24</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Haloarcula argentinensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-06-24-5/rawdata/</p>A_grid2_atlas_01_tgt.mrc,  Tilt Series (Pixel Size 0.33 nm), 564.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-06-24-5/rawdata/A_grid2_atlas_01_tgt.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5a691-8kq86
title: Amoebophilus asiaticus

description: Raw data files of Amoebophilus asiaticus
description: <strong>Tilt Series Date:</strong> 2012-03-23</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Amoebophilus asiaticus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -9.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Matthias Horn and Karin Aistleitner</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2012-03-23-9/rawdata/</p>03232012_MP78_6.mrc,  Tilt Series (Pixel Size 0.63 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2012-03-23-9/rawdata/03232012_MP78_6.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tzb3v-gme41
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-107/rawdata/</p>PginWT_8_108.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-107/rawdata/PginWT_8_108.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_108_part37_1.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-107/3dimage_114571/PginWT_8_108_part37_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sh8k9-d1j86
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-55/rawdata/</p>PginWT_8_026.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-55/rawdata/PginWT_8_026.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_026_part38_1.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-55/3dimage_114480/PginWT_8_026_part38_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.daeyhp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea trilobata",
 "Country is Armenia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002709-250827131500795/datasets/export for details.
DOI: 10.15468/dl.rvwsdq
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea trilobata",
 "Country is Norway",
 "HasCoordinate is true"
 ]
}

 The dataset includes 15 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002710-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/sqr7g-4yx84
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-25/rawdata/</p>PginWT_8_011.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-25/rawdata/PginWT_8_011.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_011_part41_10.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-25/3dimage_114419/PginWT_8_011_part41_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3j8ax-sek24
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-136/rawdata/</p>PginWT_8_134.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-136/rawdata/PginWT_8_134.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_134_part40_1.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-136/3dimage_114630/PginWT_8_134_part40_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.suvmxg
title: Occurrence Download

description: A dataset containing 113 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea trilobata",
 "Country is Sweden",
 "HasCoordinate is true"
 ]
}

 The dataset includes 113 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002711-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/274gx-wb128
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-48/rawdata/</p>PginWT_8_079.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-48/rawdata/PginWT_8_079.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_079_full.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-48/3dimage_114465/PginWT_8_079_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/agawr-bk649
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-272/rawdata/</p>PginWT_8_270.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-272/rawdata/PginWT_8_270.mrc"
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        PginWT_8_270_full.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
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