cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.26068321
title: Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d

description: In this study, the authors compared the efficiency of automated robotic and manual injection methods for the CRISPR-RfxCas13d (CasRx) system for mRNA knockdown and Cas9-mediated DNA targeting in zebrafish embryos. They targeted the no tail (<i>TBXTA</i>) gene as a proof-of-principle, evaluating the induced embryonic phenotypes. Both Cas9 and CasRx systems caused loss of function phenotypes for <i>TBXTA</i>. Cas9 protein exhibited a higher percentage of severe phenotypes compared with mRNA, while CasRx protein and mRNA showed similar efficiency. Both robotic and manual injections demonstrated comparable phenotype percentages and mortality rates. The findings highlight the potential of RNA-targeting CRISPR effectors for precise gene knockdown and endorse automated microinjection at a speed of 1.0 s per embryo as a high-throughput alternative to manual methods. The authors compared the efficiency of automated robotic and manual injection methods for the CRISPR-RfxCas13d system for mRNA knockdown and Cas9-mediated DNA targeting in zebrafish embryos. Both robotic and manual injections demonstrated comparable phenotype percentages and mortality rates. The findings show that automated microinjection is highly suitable as a high-throughput alternative to manual methods of gene silencing in zebrafish embryos. The development of an advanced robotic system with an integrated touch screen, automated droplet calculation and selective euthanasia has enhanced the efficiency and precision of zebrafish injection. Here we present a stepwise procedure showcasing the robot's efficiency of speed of 59 embryos/min and high precision in zebrafish embryo injection procedures. The robotic injections demonstrated a three-times faster injection speed, achieving a rate of 59 embryos per minute, compared to manual injections, which managed only 20 embryos per minute. Exploration of injection positions, wether at the yolk center or cell interface, was conducted to assess their impact on phenotypes; demonstating that the position of injection has not efect in the phenotype penetrance. RfxCas13d system demonstrated efficacy at the RNA level, exhibiting high phenotypic penetrance in <i>TBXTA</i> gene knockdown in both robotic and manual injections. This study contributes to understanding the potential of RNA-targeting CRISPR effectors for precise gene knockdown in a highthroughput manner. The study establishes the efficacy and advantages of robotic automated injection methods for gene silencing in zebrafish embryos, compared to manual injection methods. The robotic system offers user-friendly features, precision and unique capabilities, streamlining large-scale injections. Overall, the study advances understanding in the field, providing a promising approach for accelerating research in early embryonic development and large-scale injections; including, the use of CRISPR effectors for highthroughput genetic and transcriptomic screenings.
DOI: 10.6084/m9.figshare.26068321.v1
title: Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d

description: In this study, the authors compared the efficiency of automated robotic and manual injection methods for the CRISPR-RfxCas13d (CasRx) system for mRNA knockdown and Cas9-mediated DNA targeting in zebrafish embryos. They targeted the no tail (<i>TBXTA</i>) gene as a proof-of-principle, evaluating the induced embryonic phenotypes. Both Cas9 and CasRx systems caused loss of function phenotypes for <i>TBXTA</i>. Cas9 protein exhibited a higher percentage of severe phenotypes compared with mRNA, while CasRx protein and mRNA showed similar efficiency. Both robotic and manual injections demonstrated comparable phenotype percentages and mortality rates. The findings highlight the potential of RNA-targeting CRISPR effectors for precise gene knockdown and endorse automated microinjection at a speed of 1.0 s per embryo as a high-throughput alternative to manual methods. The authors compared the efficiency of automated robotic and manual injection methods for the CRISPR-RfxCas13d system for mRNA knockdown and Cas9-mediated DNA targeting in zebrafish embryos. Both robotic and manual injections demonstrated comparable phenotype percentages and mortality rates. The findings show that automated microinjection is highly suitable as a high-throughput alternative to manual methods of gene silencing in zebrafish embryos. The development of an advanced robotic system with an integrated touch screen, automated droplet calculation and selective euthanasia has enhanced the efficiency and precision of zebrafish injection. Here we present a stepwise procedure showcasing the robot's efficiency of speed of 59 embryos/min and high precision in zebrafish embryo injection procedures. The robotic injections demonstrated a three-times faster injection speed, achieving a rate of 59 embryos per minute, compared to manual injections, which managed only 20 embryos per minute. Exploration of injection positions, wether at the yolk center or cell interface, was conducted to assess their impact on phenotypes; demonstating that the position of injection has not efect in the phenotype penetrance. RfxCas13d system demonstrated efficacy at the RNA level, exhibiting high phenotypic penetrance in <i>TBXTA</i> gene knockdown in both robotic and manual injections. This study contributes to understanding the potential of RNA-targeting CRISPR effectors for precise gene knockdown in a highthroughput manner. The study establishes the efficacy and advantages of robotic automated injection methods for gene silencing in zebrafish embryos, compared to manual injection methods. The robotic system offers user-friendly features, precision and unique capabilities, streamlining large-scale injections. Overall, the study advances understanding in the field, providing a promising approach for accelerating research in early embryonic development and large-scale injections; including, the use of CRISPR effectors for highthroughput genetic and transcriptomic screenings.
DOI: 10.6084/m9.figshare.26372159
title: Selection of allergen extract for immunotherapy in polysensitized allergic rhinitis patients

description: <b>Aim:</b> To evaluate the criteria used by allergists in selecting an immunotherapy extract (allergen immunotherapy [AIT]-extract) in rhinitis patients with polysensitization. <b>Methods:</b> First, a cross-sectional study was carried out by evaluating different factors that influence the medical choice of AIT-extract. Second, a literature review was performed by evaluating the diagnostic performance of atopy tests. <b>Results:</b> A total of 419 patients were included (84 children, 149 adolescents and 186 adults). Anamnesis, atopy tests and exposure to pets were the main factors for choosing the AIT extract. The sensitivity and specificity of atopy tests were high for <i>Dermatophagoides spp.</i>, (&gt;80%), moderate for pets (60%) and indeterminate for <i>Blomia tropicalis</i>. <b>Conclusion:</b> NCTs could be necessary for AIT-extract selection in polysensitized allergic rhinitis patients. Allergen immunotherapy is an effective treatment for patients with allergic rhinitis. Atopy tests are used to identify possible substances in the environment that cause symptoms. A patient may sometimes have multiple substances which could be causing their allergic reactions, which makes it difficult to choose the appropriate immunotherapy for the patient. In this study, we identified some factors that might help to guide the criteria used by allergists when selecting the extract for immunotherapy. To the knowledge of the authors, this is the first study evaluating allergist criteria for the selection of an allergen immunotherapy (AIT)-extract in polysensitized patients. The principal criteria for selection of AIT-extracts were atopy tests, local allergen prevalence, anamnesis and allergen exposure. The selection of an AIT-extract based only on anamnesis and the presence of pets in homes has poor diagnostic performance. According to the nasal challenge test (NCT), for the selection of the <i>Dermatophagoides spp</i>. AIT-extract, atopy tests have a high diagnostic performance. According to the NCT, for the selection of the <i>Blomia tropicalis</i> AIT-extract, atopy tests have an indeterminate diagnostic performance. According to the NCT, for the selection of pets (cat or dog), AIT-extract atopy tests have a moderate diagnostic performance. A systematic review supports the use of the NCT for evaluating clinical relevance and choosing the AIT-extract in polysensitized AR patients. Diagnosis by components can help improve the selection of the most appropriate extract for polysensitized patients; however, there is a lack of studies that evaluate the diagnostic performance of this test for some sources of allergens.
DOI: 10.6084/m9.figshare.26372159.v1
title: Selection of allergen extract for immunotherapy in polysensitized allergic rhinitis patients

description: <b>Aim:</b> To evaluate the criteria used by allergists in selecting an immunotherapy extract (allergen immunotherapy [AIT]-extract) in rhinitis patients with polysensitization. <b>Methods:</b> First, a cross-sectional study was carried out by evaluating different factors that influence the medical choice of AIT-extract. Second, a literature review was performed by evaluating the diagnostic performance of atopy tests. <b>Results:</b> A total of 419 patients were included (84 children, 149 adolescents and 186 adults). Anamnesis, atopy tests and exposure to pets were the main factors for choosing the AIT extract. The sensitivity and specificity of atopy tests were high for <i>Dermatophagoides spp.</i>, (&gt;80%), moderate for pets (60%) and indeterminate for <i>Blomia tropicalis</i>. <b>Conclusion:</b> NCTs could be necessary for AIT-extract selection in polysensitized allergic rhinitis patients. Allergen immunotherapy is an effective treatment for patients with allergic rhinitis. Atopy tests are used to identify possible substances in the environment that cause symptoms. A patient may sometimes have multiple substances which could be causing their allergic reactions, which makes it difficult to choose the appropriate immunotherapy for the patient. In this study, we identified some factors that might help to guide the criteria used by allergists when selecting the extract for immunotherapy. To the knowledge of the authors, this is the first study evaluating allergist criteria for the selection of an allergen immunotherapy (AIT)-extract in polysensitized patients. The principal criteria for selection of AIT-extracts were atopy tests, local allergen prevalence, anamnesis and allergen exposure. The selection of an AIT-extract based only on anamnesis and the presence of pets in homes has poor diagnostic performance. According to the nasal challenge test (NCT), for the selection of the <i>Dermatophagoides spp</i>. AIT-extract, atopy tests have a high diagnostic performance. According to the NCT, for the selection of the <i>Blomia tropicalis</i> AIT-extract, atopy tests have an indeterminate diagnostic performance. According to the NCT, for the selection of pets (cat or dog), AIT-extract atopy tests have a moderate diagnostic performance. A systematic review supports the use of the NCT for evaluating clinical relevance and choosing the AIT-extract in polysensitized AR patients. Diagnosis by components can help improve the selection of the most appropriate extract for polysensitized patients; however, there is a lack of studies that evaluate the diagnostic performance of this test for some sources of allergens.
DOI: 10.15468/dl.c7ytz4
title: Occurrence Download

description: A dataset containing 2943 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Continent is Europe",
 "GadmGid is one of (GBR.3_1, GBR.4_1, GBR.1_1)",
 "Geometry POLYGON((-180 50,180 50,180 51,-180 51,-180 50))",
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Cepaea nemoralis (Linnaeus, 1758), Cepaea hortensis (O.F.Müller, 1774))"
 ]
}

 The dataset includes 2943 records from 19 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043191-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.13135576
title: Crater diameter of granular polystyrene layer with various diameter of grains and Weber number of water droplet

description: Datas about experiments of impacts made in 2021-2023. A water droplet impacting a polystyrene granular layer of 4 differents grains diameters. These datas give the crater diameter obtain in function of the Weber number of the droplet. A file from tomography of the granular layer before impact is added.
DOI: 10.5281/zenodo.13135577
title: Crater diameter of granular polystyrene layer with various diameter of grains and Weber number of water droplet

description: Datas about experiments of impacts made in 2021-2023. A water droplet impacting a polystyrene granular layer of 4 differents grains diameters. These datas give the crater diameter obtain in function of the Weber number of the droplet. A file from tomography of the granular layer before impact is added.
DOI: 10.15468/dl.erem3w
title: Occurrence Download

description: A dataset containing 20969 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Israel, Palestine, State of)",
 "DatasetKey is iNaturalist Research-grade Observations",
 "Year is 2024"
 ]
}

 The dataset includes 20969 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043192-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.26411209
title: Highreflection and texture-less workpiece dataset(HW6D) for 6D pose estimation

description: This is a selfmade datases used to evaluate the 6D pose estimation network named Swin-AFF for highreflection and texture-less workpieces. In this dataset, flange and connector are metal workpieces with reflective surfaces and symmetrical shapes, while frame and clamp are 3D printed plastic workpieces without texture, varying in sizes and shapes. The Intel RealSense D435i camera was used to collect 8 videos. The actual pose of the target workpiece in each frame was labeled, which produced approximately 6,000 labeled images for each workpiece. The dataset was divided according to LineMOD, and an additional 20,000 synthetic data were added for each object to expand the dataset
DOI: 10.6084/m9.figshare.26411209.v1
title: 用于6D位姿估计的高反射无纹理工件数据集（HW6D）

description: 这是一个自制的数据，用于评估名为 Swin-AFF 的 6D 位姿估计网络，用于高反射和无纹理工件。在这个数据集中，法兰和连接器是具有反射表面和对称形状的金属工件，而框架和夹具是没有纹理的3D打印塑料工件，大小和形状各不相同。Intel RealSense D435i 摄像头用于收集 8 个视频。对目标工件在每一帧中的实际姿态进行标记，从而为每个工件生成大约 6,000 个标记图像。根据LineMOD对数据集进行划分，并为每个对象额外添加20,000个合成数据以扩展数据集。
DOI: 10.15468/dl.jmu5ry
title: Occurrence Download

description: A dataset containing 29164 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Israel, Palestine, State of)",
 "DatasetKey is iNaturalist Research-grade Observations",
 "Year is 2023"
 ]
}

 The dataset includes 29164 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043193-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yztpz6
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((29.67615 -19.04979,30.00926 -19.04979,30.00926 -18.85066,29.67615 -18.85066,29.67615 -19.04979))",
 "HasCoordinate is true",
 "IucnRedListCategory is one of (NT, VU, EN, CR)"
 ]
}

 The dataset includes 21 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043196-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c759p9
title: Occurrence Download

description: A dataset containing 24206 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Israel, Palestine, State of)",
 "DatasetKey is iNaturalist Research-grade Observations",
 "Year is 2022"
 ]
}

 The dataset includes 24206 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043194-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7smued
title: Occurrence Download

description: A dataset containing 32048 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Israel, Palestine, State of)",
 "DatasetKey is iNaturalist Research-grade Observations",
 "Year 2020-2021"
 ]
}

 The dataset includes 32048 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043195-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8q4yyz
title: Occurrence Download

description: A dataset containing 199670 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lepomis gibbosus (Linnaeus, 1758)"
 ]
}

 The dataset includes 199670 records from 572 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043190-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6c8ssk
title: Occurrence Download

description: A dataset containing 616 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Proteus anguinus Laurenti, 1768"
 ]
}

 The dataset includes 616 records from 51 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043199-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.uv7rpf
title: Occurrence Download

description: A dataset containing 36305 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Israel, Palestine, State of)",
 "DatasetKey is iNaturalist Research-grade Observations",
 "Year 1968-2019"
 ]
}

 The dataset includes 36305 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043198-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.13142094
title: Statistical data - Waste management sector transformation and its impact towards JGT in Montenegro

description: The dataset represents statistical data collected and interpreted by the authors in the framework of the research titled "Waste Management Sector Transformation and its Impact towards JGT in Montenegro"

The dataset contains tables and graphs that are employed within the research and may be accessed by the general public in compliance with the Creative Commons Attribution license. The datasheets include:



Typical of RDF recovered from municipal solid wastes generated in industrialized nations

Waste types are used as fuels in EU-27 cement kilns

GHG performance of typical fuels

Amount of waste generated and managed per inhabitant in Montenegro

Population and waste in targeted municipalities

Amount of waste generated and collected in the study area

Number of Inhabitants in targeted municipalities

Waste quantities (in tons) in (a) Old Royal Capital Cetinje; (b) Danilovgrad and (c) Podgorica
DOI: 10.5281/zenodo.13142095
title: Statistical data - Waste management sector transformation and its impact towards JGT in Montenegro

description: The dataset represents statistical data collected and interpreted by the authors in the framework of the research titled "Waste Management Sector Transformation and its Impact towards JGT in Montenegro"

The dataset contains tables and graphs that are employed within the research and may be accessed by the general public in compliance with the Creative Commons Attribution license. The datasheets include:



Typical of RDF recovered from municipal solid wastes generated in industrialized nations

Waste types are used as fuels in EU-27 cement kilns

GHG performance of typical fuels

Amount of waste generated and managed per inhabitant in Montenegro

Population and waste in targeted municipalities

Amount of waste generated and collected in the study area

Number of Inhabitants in targeted municipalities

Waste quantities (in tons) in (a) Old Royal Capital Cetinje; (b) Danilovgrad and (c) Podgorica
DOI: 10.18150/xjg7a7
title: Data of failure of dams and reliability calculations

description: The file contains data of dam failures from the publication: ICOLD-CIGB (1995) Dam Failures. Statistiacal analysis, Bulletin no. 99; Polish edition: IMGW (2000) Katastrofy zapór, analiza statystyczna, and also calculations of mean time to failure and reliability function of dams.
DOI: 10.34943/46f2893b-939b-4a6a-a333-a9464ae3b6aa
title: Hercules Conductivity Temperature Depth Deployed 2023-06-25

description: The Sea-Bird SeaCAT SBE19plus V2 7875 was deployed on 2023-06-25 on Hercules. This Remotely Operated Vehicle is operated by Ocean Exploration Trust. This device is a Conductivity Temperature Depth. Conductivity Temperature Depth (CTD) is an instrument package that contains sensors for measuring the conductivity, temperature, and pressure of seawater. Salinity, sound velocity, depth and density are variables that can be derived from sensor measurements. CTDs can carry additional instruments and sensors such as oxygen sensors, turbidity sensors and fluorometers. It was deployed on a mobile platform. Data from this deployment were archived and made available through Ocean Networks Canada's Oceans 3.0 digital infrastructure, with quality assurance and derived data products following established practices.
DOI: 10.34943/f93f2b9c-2a3b-4b0e-9a08-e0e8702a518c
title: Hercules Oxygen Sensor Deployed 2023-06-25

description: The Sea-Bird SBE 43 Oxygen (S/N 4239) was deployed on 2023-06-25 on Hercules. This Remotely Operated Vehicle is operated by Ocean Exploration Trust. This device is a Oxygen Sensor. Oxygen sensors measure dissolved oxygen concentration in seawater. It was deployed on a mobile platform. Data from this deployment were archived and made available through Ocean Networks Canada's Oceans 3.0 digital infrastructure, with quality assurance and derived data products following established practices.
DOI: 10.34943/236f094d-fe2a-4758-9b71-7dacd3293726
title: Hercules Navigation System Deployed 2023-06-25

description: The Hercules ROV Navigation 01 was deployed on 2023-06-25 on Hercules. This Remotely Operated Vehicle is operated by Ocean Exploration Trust. This device is a Navigation System. Navigational instruments are used to measure and record positional information of ships, buoys, and autonomous underwater vehicles (AUVs). Typical sensors include latitude, longitude, speed, heading, pitch, roll, and depth. It was deployed on a mobile platform. Data from this deployment were archived and made available through Ocean Networks Canada's Oceans 3.0 digital infrastructure, with quality assurance and derived data products following established practices.