DOI: 10.6084/m9.figshare.11297843
title: Data on the antiviral and antioxidant activity of seven Thai medicinal plant extracts on the Porcine Reproductive and Respiratory Syndrome Virus

description: This dataset consists of four Excel <b>.xlsx</b> format spreadsheets. These are <b>virus titer_virus infection.xlsx</b>, <b>virus titer_replication.xlsx</b>, <b>phytochemical.xlsx </b>and <b>Cytotoxic.xlsx</b>.<br>Dataset <b>Cytotoxic.xlsx </b>shows data on the cytotoxic activity of the seven Thai medicinal plant extracts on the viability of MARC-145 cells. This was determined by the MTT assay. MARC-145 cells were incubated with various concentrations of the seven Thai medicinal plant extracts for 72 h prior to the MTT assay. Viability is shown as 50% cytotoxic concentrations (cc50). The cytotoxic concentration cc50 of the seven Thai medicinal plant extracts ranged from 78 ug/ml to 2,500 ug/ml. Average cytotoxic concentrations cc50 of each plant are shown in column 6, and standard deviation (SD) values are shown in column 7.<br>Dataset <b>phytochemical.xlsx </b>consists of 4 sheets labeled "Total phenol", "DPPH", "ABTS" and "Sheet 4". Sheet "Total phenol" shows the total phenol content of the of the seven Thai medicinal plant extracts. Total phenol content was calculated in micromoles (mM) gallic acid equivalents (GAE) per gram of plant extract (mM GAE/1g extract). Average and standard deviation values of total phenol content are shown in columns 6 and 7 respectively.Sheet "DPPH" shows data on the antioxidant activity of the 7 plant extracts. The free radical, 2, 2-Diphenyl-1- picrylhydrazyl (DPPH) is widely used to test the ability of compounds to act as free radical scavengers, and therefore to evaluate their antioxidant activity. Antioxidant activity was measured as the half maximal inhibitory concentration (IC50) of each extract in milligrams per milliliter (mg/ml). Average and standard deviation (SD) values are shown.Sheet "ABTS" shows data on the antioxidant activity of the 7 plant extracts. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay measures the relative ability of an antioxidant to scavenge the ABTS generated in aqueous phase. Antioxidant activity was measured as the half maximal inhibitory concentration (IC50) of each extract in milligrams per milliliter (mg/ml). Average and standard deviation (SD) values are shown.Sheet "FRAP" shows the results of the Ferric reducing antioxidant power (FRAP) assay using the 7 plant extracts. The assay uses antioxidants (in this case the plant extracts) as reductants in a redox-linked colorimetric reaction, wherein Fe<sup>3+</sup> is reduced to Fe<sup>2+ </sup>. Antioxidant activity was measured in millimolar concentration of Fe<sup>2+</sup> per gram of plant extract (mM Fe<sup>2+</sup>/g). Average and standard deviation (SD) values are shown.<br>Dataset <b>virus titer_replication.xlsx </b>consists of 7 sheets labeled "CS", "GM", "HC", "CN", "PF", "PE" and "TT" and shows the virus titer in the inhibition of viral replication activity of the Thai medicinal plant extracts against PRRSV at 24, 48, and 72-h post-infection (hpi). The MARC-145 cellswere infected with PRRSV for 1 h and then placed in a medium containing the Thai medicinal plant extracts at various concentrations according to CC50 and incubated at24, 48, and 72 h, respectively. Viral titres were measured in Median Tissue Culture Infectious Dose (TCID50). TCID50 signifies the concentration at which 50% of the cells are infected. Each sheet shows data on the antiviral activity of each of the 7 plant extracts. <br>Dataset <b>virus titer_virus infection.xlsx </b>shows data on the virus titer of the inhibition of the viral infection activity of the seven Thai medicinal plant extracts against PRRSV at 24-h post infection (hpi). MARC-145 cells were infected with the Porcine reproductive and respiratory syndrome virus (PRRSV) at a multiplicity of infection of 1. Plant extracts were incubated with PRRSV for 1 h at 37°C before inoculation onto a monolayer of MARC-145 cells. Virus titres were quantified by the immunoperoxidase monolayer assay (IPMA). Virus titres were measured in TCID50.<b><br></b><b>Study aims and methodology:</b> The aim of this study was to determine the antiviral activities of seven Thai medicinal plant extracts against PRRSV infection <i>in vitro</i> and to measure their phytochemical contents to develop an alternative anti-PRRSV therapy for use in veterinary medicine.The Thai medicinal plants used in this study were Ethanolic extracts from the Thai medicinal plants <i>C. sappan</i> Linn., <i>G. mangostana </i>Linn., <i>H. cordata</i>, <i>P. frutescens</i>, <i>C. nutans</i>, <i>P. emblica</i>, and <i>T. triandra. </i>The cytotoxicity of the seven Thai medicinal plant extracts was determined on MARC-145 cells using the MTT cell viability assay. The following procedures are described in more detail in the published article: Inhibition of virus infection assay, Inhibition of virus replication assay, Determination of virus titer, phytochemical analysis and statistical analysis. <br>