DOI: 10.5061/dryad.223p4
title: Data from: Multiple reproductive barriers separate recently diverged sunflower ecotypes

description: Measuring reproductive barriers between groups of organisms is an
 effective way to determine the traits and mechanisms that impede gene
 flow. However, to understand the ecological and evolutionary factors that
 drive speciation, it is important to distinguish between the barriers that
 arise early in the speciation process and those that arise after
 speciation is largely complete. In this paper we comprehensively test for
 reproductive isolation between recently diverged (&lt; 10,000 years
 bp) dune and non-dune ecotypes of the prairie sunflower, Helianthus
 petiolaris. We find reproductive barriers acting at multiple stages of
 hybridization, including premating, postmating-prezygotic, and postzygotic
 barriers, despite the recent divergence. Barriers include extrinsic
 selection against immigrants and hybrids, a shift in pollinator
 assemblage, and post-pollination assortative mating. Together these data
 suggest that multiple barriers can be important for reducing gene flow in
 the earliest stages of speciation.
description: 2012_RT_PlotsThe number of seedlings that emerged and survived in each subplot of our 2012 reciprocal transplant in which we planted dune, non-dune and hybrid seeds into a site in the dunes and a site in the non-dune habitat (sand sheet) at Great Sand Dunes National Park and Preserve.2012_RT_Plants_fullData (height, day to first flower, number of seeds, etc) for each plant that survived in our 2012 reciprocal transplant2012_RT_AsterData (emergence, survival, number of flowers, number of seeds) for each seed planted in our 2012 reciprocal transplant formatted for an ASTER analysis2012_RT_FT_survivalThe period during which each plant that survived in our 2012 reciprocal transplant flowered (formatted for a survival analysis)2012_Timelapse_FT_numbersThe number of flowers photographed by 8 timelapse cameras (DG4, DG5, Updune, 1363, 2001, NDG2, NDG3, NDG4) each day. Cameras DG4 and DG5 were in the same site, which is called DRT. Cameras NDG2, NDG3 and NDG4 were in the same site, which is called NRT. See Fig S1 for site information.2012_PollinatorsCollection information and (morpho)species identifications for potential pollinators collected at Great Sand Dunes National Park and Preserve2014_PCO_genoytpes_fullThe number of offspring with particular genotypes (NN, NC or CC) that resulted from pollinations with mixtures of dune and non-dune pollen (dune pollen contributes "C" alleles and non-dune pollen contributes "N" alleles)2014_Seed_setThe number of seeds set after pollinating 30 stigmas on dune and non-dune maternal plants with either dune pollen or non-dune pollen2014_PCO_6and12_genotypesThe number of offspring fathered by dune or non-dune pollen after different pollination treatments (1 = dune pollen applied 12 hours before non-dune pollen, 2 = dune pollen applied 6 hours before non-dune pollen, 3 = dune and non-dune pollen applied together, 4 = non-dune pollen applied 6 hours before dune pollen, 5 = non-dune pollen applied 12 hours before dune pollen)2014_GerminationData on whether seeds from crosses made within and between dune and non-dune ecotypes (in the greenhouse in 2014) germinatedHybrid_pollenData on the viability of pollen produced by dune, non-dune and hybrid plants2014_Seed_Size_Common_GardenThe weight of seeds produced by dune and non-dune maternal plants pollinated with dune and non-dune pollen2010_RTThe number of seedlings that emerged in each subplot of our 2010 reciprocal transplant in which we planted dune, non-dune and intermediate (collected at the boundary between dune and non-dune habitat) seeds into a site in the dunes and a site in the non-dune habitat (sand sheet) at Great Sand Dunes National Park and Preserve.2010_RT_longData (emergence, survival, height, number of flowers, head diameter) for each seed planted in our 2010 reciprocal transplant2011_RTThe number of seedlings that emerged in each subplot of our 2011 reciprocal transplant in which we planted dune and non-dune seeds into a site in the dunes and a site in the non-dune habitat (sand sheet) at Great Sand Dunes National Park and Preserve.2011_GerminationData on whether seeds from crosses made within and between dune and non-dune ecotypes (in the field in 2011) and seeds from open pollinated plants germinated2008_PopulationsLatitude and longitude information for each population used in this studyGSD_Barriers_RcodeThe R code used to produce each statistical analysis and figure presented in this manuscript. The code is presented in the same order that the analyses are presented in the manuscript.