cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/9ygab-83425
title: Pseudoalteromonas Iuteoviolacea MP129

description: P Iuteo DvipABhcpI Dbact2 whole cells from liquid culture
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab, taken at Janelia Farms</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid culture</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-27/rawdata/</p>2013_07_12_JF_MP129_b_0022.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-27/rawdata/2013_07_12_JF_MP129_b_0022.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_07_12_JF_MP129_b_0022_2k_secs_full.rec,  Reconstruction (Pixel Size 1.68 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-27/3dimage_28967/2013_07_12_JF_MP129_b_0022_2k_secs_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qd39k-tr629
title: Vibrio delta-CheA1

description: Vibrio cholerae strain deleted for CheA1
description: <strong>Tilt Series Date:</strong> 2014-03-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Ikuro Kawagishi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-04-30/rawdata/</p>deltaA1VibrioC0030.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-04-30/rawdata/deltaA1VibrioC0030.mrc"
        > <i class="download icon"></i> Download </a></p>

        deltaA1VibrioC0030_part121_34.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-04-30/3dimage_39664/deltaA1VibrioC0030_part121_34.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m9vfn-0an64
title: Agro C1 induction 28C

description: Agro C1, no Ti plasmid, induced at 28 for 24h in liquid AB+AS
description: <strong>Tilt Series Date:</strong> 2014-03-25</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Agro C1, no Ti plasmid, induced at 28 in liquid AB+AS</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-25-7/rawdata/</p>Agro_C1_Pat0007.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-25-7/rawdata/Agro_C1_Pat0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        Agro_C1_Pat0007.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-25-7/3dimage_49438/Agro_C1_Pat0007.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d2a55-05j35
title: HeLa cells

description: Looking for ESCRT near midbodies
description: <strong>Tilt Series Date:</strong> 2014-12-08</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> with Catherine Ullman and Doug mckay</p>
<strong>Sample Preparation:</strong> siRNA NUP 153+Thymidine for 14h,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-12-08-9/rawdata/</p>midbody20007.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-12-08-9/rawdata/midbody20007.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody20007_part121_46.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-12-08-9/3dimage_48646/midbody20007_part121_46.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4fczp-08r62
title: Hyphomonas EB session21 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-07-14</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, EB 0.02mg/ml overnight 4degree...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
  jsa2009-07-14-46_slicer8891.jpg: Hyphomonas neptunium .  DNA treated with EtBr .  Supercoil
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-07-14-46/rawdata/</p>09jul14a_b200c_046.mrc,  Tilt Series (Pixel Size 0.632 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-07-14-46/rawdata/09jul14a_b200c_046.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jul14a_b200c_046_part.rec,  Reconstruction (Pixel Size 1.264 nm), 367.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-07-14-46/3dimage_8270/09jul14a_b200c_046_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1dq9e-jq175
title: Hyphomonas chq session19 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-06-25</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 2°, constant angular increment, dosage: 75.0 eV/Å²,  defocus: -7.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, chloroquine 1.0mg/ml, 4degree...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
  jsa2009-06-25-30_slicer8772.jpg: Tube .  Hyphomonas neptunium
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-25-30/rawdata/</p>09jun25a_bx217d_066.mrc,  Tilt Series (Pixel Size 0.632 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-25-30/rawdata/09jun25a_bx217d_066.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun25a_bx217d_066_full.rec,  Reconstruction (Pixel Size 1.264 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-25-30/3dimage_8130/09jun25a_bx217d_066_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qqmy4-7wr11
title: PVA 032409 609

description: Prosthecobacter vanneervenii, 09apr03a_Pvashake609_2_004,
description: <strong>Tilt Series Date:</strong> 2009-04-03</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Prosthecobacter vanneervenii</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-04-03-4/rawdata/</p>09apr03a_Pvashake609_2_004.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-04-03-4/rawdata/09apr03a_Pvashake609_2_004.mrc"
        > <i class="download icon"></i> Download </a></p>

        09apr03a_Pvashake609_2_004_full.rec,  Reconstruction (Pixel Size 1.9 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-04-03-4/3dimage_13778/09apr03a_Pvashake609_2_004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/snp9h-rbt45
title: Agrobacterium tumefaciens

description: VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB + antibiotics</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-1/rawdata/</p>vg3101_20001.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-1/rawdata/vg3101_20001.mrc"
        > <i class="download icon"></i> Download </a></p>

        vg3101_20001_part111_1.rec,  Reconstruction (Pixel Size 0.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-1/3dimage_48936/vg3101_20001_part111_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.86pzhv
title: Occurrence Download

description: A dataset containing 11 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Macrhybopsis meeki)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 11 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002259-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ft3dt-j3n14
title: Agrobacterium tumefaciens

description: Agrobacterium_VirB8-GFP_deltaVirB8
description: <strong>Tilt Series Date:</strong> 2013-05-07</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from PatZambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Titan_K2</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-39/rawdata/</p>VirB_deltaVirB0001.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-39/rawdata/VirB_deltaVirB0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        VirB_deltaVirB0001_part123_27.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-39/3dimage_32406/VirB_deltaVirB0001_part123_27.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wp5gg-3ng04
title: Bsubtilis349

description: Bsubtilis JFG349 (MJ007, SpoIIIE78-11-GFP)
description: <strong>Tilt Series Date:</strong> 2011-08-08</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> JFG349, ponA null, SpoIIIE73-11-GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-08-11/3dimage_24846/</p>3490011_full.rec,  Reconstruction (Pixel Size 1.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-08-11/3dimage_24846/3490011_full.rec"
        > <i class="download icon"></i> Download </a></p>

        3490011.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-08-11/rawdata/3490011.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.93ungw
title: Occurrence Download

description: A dataset containing 251 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Argentina",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Ozotoceros bezoarticus"
 ]
}

 The dataset includes 251 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002258-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/wmtqk-3dp47
title: Alongum

description: Alongum_periplasmiclayers
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-17/rawdata/</p>AlongLys0019.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-17/rawdata/AlongLys0019.mrc"
        > <i class="download icon"></i> Download </a></p>

        AlongLys0019_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-17/3dimage_56754/AlongLys0019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6rawz-60e76
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN and pNLGFP provirus construct
description: <strong>Tilt Series Date:</strong> 2012-04-25</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-1/3dimage_68532/</p>12apr25_C123_3_Cos7pNLDN_AT2BT3_0001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-1/3dimage_68532/12apr25_C123_3_Cos7pNLDN_AT2BT3_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr25_C123_3_Cos7pNLDN_AT2BT3_0001.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zcba1-gy221
title: HUVECs infx pNLGFP

description: Raw data files of HUVECs infx pNLGFP
description: <strong>Tilt Series Date:</strong> 2013-09-11</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 24h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-09-11-1/rawdata/</p>13sep11_C167-1_HUVECspnl24hPI_montage27X27_0002.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
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        13sep11_C167-1_HUVECspnl24hPI_montage27X27_0002_full.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
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