cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8128139.v1
title: Dietary Effects of Natural Polyphenol Antioxidant on Laying Performance and Egg Quality of Laying Hens Fed Diets with Oxidized Oil

description: ABSTRACT This study was conducted to investigate the effects of dietary oxidized oil and natural polyphenol antioxidants on laying performances and egg quality of laying hens. A total of two hundred, 61-week-old Lohmann Brown laying hens were divided into five groups, consisting in four replicates of 10 birds each. They were fed one of the five experimental diets (fresh oil only, oxidized oil without antioxidants, oxidized oil with vitamin E 200 ppm, oxidized oil with vitamin E 100 ppm + Cabanin CSDⓇ 100 ppm, oxidized oil with Cabanin CSDⓇ 500 ppm)respectively, for 6 weeks. There were no significant differences in laying performances and egg weight among the treatments. However, egg mass in group fed oxidized oil with vitamin E 100 ppm + Cabanin CSDⓇ 100 ppmwas significantly higher than group fed oxidized oil only (p&lt;0.05). Eggshell thickness and eggshell strength was not affected by feeding of oxidized oil and antioxidants. Haugh unit checked after storage of 14 d from the end date of the trial showed significant difference (p&lt;0.05). Serum superoxide dismutase (SOD) like activity was significantly higher in antioxidant treated groups. The level of serum glutamic oxaloacetic transaminase (GOT) was significantly lower in groups fed vitamin E 100 ppm + Cabanin CSDⓇ 100 ppm or Cabanin CSDⓇ 500 ppm compared to groups fed oxidized oil without antioxidant. The number of total microbes, lactobacilli, and Enterocossus faecium showed significant difference favored to antioxidants groups. This study indicates that addition of oxidized oil to layer diet may have harmful effect on oxidative status and egg quality, but concurrent addition of vitamin E and natural polyphenol can alleviate the toxic effect of oxidized oil.
DOI: 10.6084/m9.figshare.8128136.v1
title: Ethnographic approach and social intervention in the production and use of images: interview with José Roberto Novaes (Beto Novaes)

description: Abstract Beto Novaes is a retired professor of the Institute of Economics of the Federal University of Rio de Janeiro, where he coordinates the ‘Education through Images’ project. Since the mid-seventies, he has undertaken research and extension activities based on the use of images about work and workers, mostly in the rural sector. Images are the means used to bring research and academic knowledge closer to society, disrupting the hierarchy of knowledge in which academic knowledge is normally considered superior. His productions arise from social demands to deal with problems of little visibility such as: the exploitation of labor and migrations in the rural domain, child and female labor, workers’ resistance, agroecology and confrontation with pesticides use. Through his ethnographic approach, he acts against the exercise of domination through workers’ narratives, ‘giving voice to those who have no voice’, and designing ways of confronting with and changing reality. His documentaries express teachings in the art of ethnography and in the practice of intervention, not only with a view to change social reality, but also to influence academic praxis. His films are also artistic pieces where beauty coexists with sensitivity, generosity, commitment and hope.
DOI: 10.6084/m9.figshare.8128130
title: Duplex Real-Time PCR Using Sybr Green I for Quantification and Differential Diagnosis between Salmonella Enteritidis and Salmonella Typhimurium

description: ABSTRACT The incidence of foodborne diseases caused by the genus Salmonella spp. in industrialized countries is often high in epidemiological surveys. Obtaining a rapid diagnostic test for identification of bacteria is crucial in order to rapidly implement control measures to contain bacterial spread, to reduce losses in animal production and to avoid risks from food-borne infections to human health. The aim of this study was to standardize duplex real-time PCR using SYBr Green I for differential and quantitative diagnosis of S. Typhimurium and S. Enteritidis. According to the experiment, the melting temperature of 85°C was observed for a 206bp amplified product when S. Enteritidis DNA was added to the reaction. S. Typhimurium DNA showed that the melting temperature of 79°C when observed for a 62bp amplified product. The standard curve showed the high sensitivity of the proposed test, since it was possible to obtain eight quantification points, starting at 108 CFU/mL and ending at 101 CFU/mL. As a result of the present study, a real-time PCR duplex reaction with high sensitivity, specificity and based on the fluorescence of SYBr Green I was standardized. In addition, this methodology aligns low cost to the faster diagnostic result, in relation to other molecular tests, making it attractive for application in routine laboratory analyzes.
DOI: 10.6084/m9.figshare.8128130.v1
title: Duplex Real-Time PCR Using Sybr Green I for Quantification and Differential Diagnosis between Salmonella Enteritidis and Salmonella Typhimurium

description: ABSTRACT The incidence of foodborne diseases caused by the genus Salmonella spp. in industrialized countries is often high in epidemiological surveys. Obtaining a rapid diagnostic test for identification of bacteria is crucial in order to rapidly implement control measures to contain bacterial spread, to reduce losses in animal production and to avoid risks from food-borne infections to human health. The aim of this study was to standardize duplex real-time PCR using SYBr Green I for differential and quantitative diagnosis of S. Typhimurium and S. Enteritidis. According to the experiment, the melting temperature of 85°C was observed for a 206bp amplified product when S. Enteritidis DNA was added to the reaction. S. Typhimurium DNA showed that the melting temperature of 79°C when observed for a 62bp amplified product. The standard curve showed the high sensitivity of the proposed test, since it was possible to obtain eight quantification points, starting at 108 CFU/mL and ending at 101 CFU/mL. As a result of the present study, a real-time PCR duplex reaction with high sensitivity, specificity and based on the fluorescence of SYBr Green I was standardized. In addition, this methodology aligns low cost to the faster diagnostic result, in relation to other molecular tests, making it attractive for application in routine laboratory analyzes.
DOI: 10.6084/m9.figshare.8128127
title: Effect of Origanum syriacum L. Essential Oil on the Storage Stability of Cooked Chicken Meat

description: ABSTRACT The effects of the addition of Origanum syriacum L. essential oil (OE) on the lipid and protein oxidation, and sensory attributes of cooked chicken meat were compared with those of synthetic commercial meat preservatives. Ground deboned and skinless chicken breast and thigh meat were distributed according to six treatments: (T1) control (no addition of meat preservative); or the addition of (T2) 100 ppm OE; (T3) 150 ppm OE; (T4) 300 ppm L-ascorbic acid (E-300); (T5) 5 and 14 ppm butylated hydroxyanisoleadded to breast and thigh meat, respectively, (BHA/E-320); and (T6) 150 ppm sodium nitrite (E-250). Meat samples were cooked and analyzed for lipid oxidation (TBARS levels) and protein oxidation (carbonyl levels) on days 0, 4, and 7 days of storage. In addition, cooked meat thigh patties were evaluated for cooking loss and sensory attributes. All additives were showed significant lipid and protein antioxidant effects (p&lt;0.05) compared with the control treatment during storage, with the strongest effects obtained with OEat 150 ppm and E-250. Cooking loss was not influenced (p&gt;0.05) by the treatments. The best sensory attribute scores were obtained with OEat 150 ppm and E-250 treatments. L-ascorbic acid and BHA also showed significant effect (p&lt;0.05) on both lipid and protein oxidation values, and sensory attributes. Based on the results study, it concluded that OEat 150 ppm may be used in replacement of synthetic antioxidants to improve the storage stability of chicken meat.
DOI: 10.6084/m9.figshare.8128127.v1
title: Effect of Origanum syriacum L. Essential Oil on the Storage Stability of Cooked Chicken Meat

description: ABSTRACT The effects of the addition of Origanum syriacum L. essential oil (OE) on the lipid and protein oxidation, and sensory attributes of cooked chicken meat were compared with those of synthetic commercial meat preservatives. Ground deboned and skinless chicken breast and thigh meat were distributed according to six treatments: (T1) control (no addition of meat preservative); or the addition of (T2) 100 ppm OE; (T3) 150 ppm OE; (T4) 300 ppm L-ascorbic acid (E-300); (T5) 5 and 14 ppm butylated hydroxyanisoleadded to breast and thigh meat, respectively, (BHA/E-320); and (T6) 150 ppm sodium nitrite (E-250). Meat samples were cooked and analyzed for lipid oxidation (TBARS levels) and protein oxidation (carbonyl levels) on days 0, 4, and 7 days of storage. In addition, cooked meat thigh patties were evaluated for cooking loss and sensory attributes. All additives were showed significant lipid and protein antioxidant effects (p&lt;0.05) compared with the control treatment during storage, with the strongest effects obtained with OEat 150 ppm and E-250. Cooking loss was not influenced (p&gt;0.05) by the treatments. The best sensory attribute scores were obtained with OEat 150 ppm and E-250 treatments. L-ascorbic acid and BHA also showed significant effect (p&lt;0.05) on both lipid and protein oxidation values, and sensory attributes. Based on the results study, it concluded that OEat 150 ppm may be used in replacement of synthetic antioxidants to improve the storage stability of chicken meat.
DOI: 10.6084/m9.figshare.8128124
title: Oxidative Stress of in-Ovo Ochratoxin A Administered during Chick Embryonic Development

description: ABSTRACT The present work was carried out to study the effect of in-ovo injection of ochratoxin A (OTA) as an oxidative stress and its consequences on hepatic and kidney functions, thyroid activity, and histological examination of brain and liver in chicken embryos and subsequently in the hatching chicks. On the 10th day of incubation, one hundred and sixty-two fertile eggs were randomly divided into two equal treatments. Control treatment, (injected by 50 µl sodium carbonate) and OTA treatment (injected by 12.5 ng OTA dissolved in 50 µl sodium carbonate). OTA treatement group significantly reduced glutathione (GSH) and significantly increased thiobarbituric acid reactive substances production (TBARS) in embryonic and hatched chicks regarding livers, spleen, bursa of Fabricius, heart, and brain as an indicator of oxidative stress. OTA injection increased TBARS and decreased GSH levels in both allantoic and amniotic fluids. On the 14th and 16th days of incubation and at the hatch, a significant lower concentration in cholesterol and higher concentrations of alanine amino transferase, aspartate amino transferase, alkaline phosphatase, gamma glutamyl transferase, acid phosphatase enzymes activities and triglycerides in the hepatic tissues of the OTA group were observed. Histological examination of OTA group of brain and liver tissues showed some degenerative changes through the experimental period. In conclusion, in-ovo OTA treated had teratogenic and embryotoxic effects.
DOI: 10.6084/m9.figshare.8128124.v1
title: Oxidative Stress of in-Ovo Ochratoxin A Administered during Chick Embryonic Development

description: ABSTRACT The present work was carried out to study the effect of in-ovo injection of ochratoxin A (OTA) as an oxidative stress and its consequences on hepatic and kidney functions, thyroid activity, and histological examination of brain and liver in chicken embryos and subsequently in the hatching chicks. On the 10th day of incubation, one hundred and sixty-two fertile eggs were randomly divided into two equal treatments. Control treatment, (injected by 50 µl sodium carbonate) and OTA treatment (injected by 12.5 ng OTA dissolved in 50 µl sodium carbonate). OTA treatement group significantly reduced glutathione (GSH) and significantly increased thiobarbituric acid reactive substances production (TBARS) in embryonic and hatched chicks regarding livers, spleen, bursa of Fabricius, heart, and brain as an indicator of oxidative stress. OTA injection increased TBARS and decreased GSH levels in both allantoic and amniotic fluids. On the 14th and 16th days of incubation and at the hatch, a significant lower concentration in cholesterol and higher concentrations of alanine amino transferase, aspartate amino transferase, alkaline phosphatase, gamma glutamyl transferase, acid phosphatase enzymes activities and triglycerides in the hepatic tissues of the OTA group were observed. Histological examination of OTA group of brain and liver tissues showed some degenerative changes through the experimental period. In conclusion, in-ovo OTA treated had teratogenic and embryotoxic effects.
DOI: 10.6084/m9.figshare.8128121
title: Vegetable Waste Inclusion in Broiler Diets and its Effect on Growth Performance, Blood Metabolites, Immunity, Meat Mineral Content and Lipid Oxidation Status

description: ABSTRACT The aim of present study was to evaluate the effect of feeding vegetable waste (VW) to broilers on their growth performance, serum chemistry, immune status, meat mineral content and lipid oxidation status. For this purpose, 100 one-day-old chicks (Cobb 500) were acquired from a commercial hatchery and allocated according to a completely randomized design into five dietary treatments with four replicates of five birds each. The dietary treatments included: T1 ( 100 % c o m m e r c i a l f e e d ( C F ) + 0 % V W), T2 ( 75 % C F + 25 % V W), T3 ( 50 % C F + 50 % V W), T4 ( 25 % C F + 75 % V W) and T5 ( 0 % C F + 100 % V W). Experimental birds were subjected to dietary treatments from 5 to 7 weeks of age. At the end of week 7 (49 days), eight birds with uniform average body weight were selected per treatment (2birds/replicate), kept off-feed for 4 h and then manually slaughtered according to the Halal method to collect data for serum chemistry, meat minerals and lipid oxidation status. The results indicated better meat lipid oxidation status (p≤0.05) and lower meat mineral content (p≤0.05) when birds fed VW at 25, 50, 75, and 100% of the diets compared with 100% commercial feed. On the other hand, blood chemistry and antibody response parameters did not respond (p&gt;0.05) to dietary intervention. In conclusion, dietary inclusion of vegetable waste had positive influence on meat quality in terms of meat lipid oxidation and meat mineral content, and may be replace up to 75% of commercial broiler feeds with beneficial effects.
DOI: 10.6084/m9.figshare.8128121.v1
title: Vegetable Waste Inclusion in Broiler Diets and its Effect on Growth Performance, Blood Metabolites, Immunity, Meat Mineral Content and Lipid Oxidation Status

description: ABSTRACT The aim of present study was to evaluate the effect of feeding vegetable waste (VW) to broilers on their growth performance, serum chemistry, immune status, meat mineral content and lipid oxidation status. For this purpose, 100 one-day-old chicks (Cobb 500) were acquired from a commercial hatchery and allocated according to a completely randomized design into five dietary treatments with four replicates of five birds each. The dietary treatments included: T1 ( 100 % c o m m e r c i a l f e e d ( C F ) + 0 % V W), T2 ( 75 % C F + 25 % V W), T3 ( 50 % C F + 50 % V W), T4 ( 25 % C F + 75 % V W) and T5 ( 0 % C F + 100 % V W). Experimental birds were subjected to dietary treatments from 5 to 7 weeks of age. At the end of week 7 (49 days), eight birds with uniform average body weight were selected per treatment (2birds/replicate), kept off-feed for 4 h and then manually slaughtered according to the Halal method to collect data for serum chemistry, meat minerals and lipid oxidation status. The results indicated better meat lipid oxidation status (p≤0.05) and lower meat mineral content (p≤0.05) when birds fed VW at 25, 50, 75, and 100% of the diets compared with 100% commercial feed. On the other hand, blood chemistry and antibody response parameters did not respond (p&gt;0.05) to dietary intervention. In conclusion, dietary inclusion of vegetable waste had positive influence on meat quality in terms of meat lipid oxidation and meat mineral content, and may be replace up to 75% of commercial broiler feeds with beneficial effects.
DOI: 10.6084/m9.figshare.8128118
title: History education and educational competences

description: ABSTRACT Results from a study carried out in the Spanish universities of Murcia and Santiago de Compostela are discussed. The objective is to examine the perceptions and pedagogical content knowledge about history of pre-service primary teachers. The study yielded statistically significant results, which allowed a diagnostic about the professional competence of future primary teachers. Quantitative methods have been employed, and their use in an exploratory study is justified. The findings show that a majority of participants identify themselves, formally, with a model of critical teacher, who should use active teaching strategies and promote ethical values related to social justice. This formal stance stands in contrast with a) their implicit teaching models, more traditional in their theoretical and methodological conceptions; and b) with their selection of contents and practices in order to achieve an education coherent with the model formally endorsed. The discussion suggests the need for improvement in teacher’s education, establishing direct connections with the practicum in primary classrooms. We propose a teacher education model based on reflection about practice from practice, connected to theory, in order to educate competent primary teachers.
DOI: 10.6084/m9.figshare.8128118.v1
title: History education and educational competences

description: ABSTRACT Results from a study carried out in the Spanish universities of Murcia and Santiago de Compostela are discussed. The objective is to examine the perceptions and pedagogical content knowledge about history of pre-service primary teachers. The study yielded statistically significant results, which allowed a diagnostic about the professional competence of future primary teachers. Quantitative methods have been employed, and their use in an exploratory study is justified. The findings show that a majority of participants identify themselves, formally, with a model of critical teacher, who should use active teaching strategies and promote ethical values related to social justice. This formal stance stands in contrast with a) their implicit teaching models, more traditional in their theoretical and methodological conceptions; and b) with their selection of contents and practices in order to achieve an education coherent with the model formally endorsed. The discussion suggests the need for improvement in teacher’s education, establishing direct connections with the practicum in primary classrooms. We propose a teacher education model based on reflection about practice from practice, connected to theory, in order to educate competent primary teachers.
DOI: 10.6084/m9.figshare.8128115
title: Number of stems and plant density in mini watermelon grown in a protected environment

description: ABSTRACT Watermelon farming has a high economic and social importance. In parallel, the consumers’ demand for distinctive products has led to niche markets, where mini watermelons stand out. This study aimed to assess the agronomic performance and fruit quality of mini watermelon grown in coconut husk fiber, as a function of number of stems per plant and plant density. The Smile hybrid was used, as well as a randomized block design, in a 2 x 2 factorial scheme, with eight repetitions, being the first factor the number of stems per plant (1 or 2) and the second the number of plants per pot (1 or 2). Characteristics related to yield, physiological parameters and fruit quality were assessed. Interaction between number of stems and plant density was only observed for leaf area, average fruit fresh weight, total yield and percentage of large fruits. The factors were evaluated separately for the remaining traits. Two-stemmed training systems, at a density of one plant per pot, produce the highest yield, without compromising the quality of the fruits, being, in these conditions, the most suitable method for farmers.
DOI: 10.6084/m9.figshare.8128115.v1
title: Number of stems and plant density in mini watermelon grown in a protected environment

description: ABSTRACT Watermelon farming has a high economic and social importance. In parallel, the consumers’ demand for distinctive products has led to niche markets, where mini watermelons stand out. This study aimed to assess the agronomic performance and fruit quality of mini watermelon grown in coconut husk fiber, as a function of number of stems per plant and plant density. The Smile hybrid was used, as well as a randomized block design, in a 2 x 2 factorial scheme, with eight repetitions, being the first factor the number of stems per plant (1 or 2) and the second the number of plants per pot (1 or 2). Characteristics related to yield, physiological parameters and fruit quality were assessed. Interaction between number of stems and plant density was only observed for leaf area, average fruit fresh weight, total yield and percentage of large fruits. The factors were evaluated separately for the remaining traits. Two-stemmed training systems, at a density of one plant per pot, produce the highest yield, without compromising the quality of the fruits, being, in these conditions, the most suitable method for farmers.
DOI: 10.6084/m9.figshare.8128112
title: Results of Scharioth macular lens implant in patients with age-related macular degeneration

description: Abstract Objective: To describe the outcomes in terms of near visual acuity and quality of life in 10 patients submitted to Scharioth Macular Lens implantation. Setting: João Eugênio Eye Institute, Brasilia, Brazil. Design: Uncontroled clinical trial. Methods: Ten pseudophakic patients with DMRI were submitted to the implantation of intraocular SML in the dominant eye in the period of December of 2017 to the March of 2018. All the patients had answered the Low-Vision Quality of Life Questionaire (LVQOLQ) before and after the surgery, with interval of 30 days. Results: The results points showed a statistical significant improvement (p&lt;0.001) in the near visual acuity (NVA), without interference in the distance vision. All patients had significant improvements (p&lt;0,05) in LVQOLQ on the item that evaluates the near visual acuity. Conclusion: There was an improvement of NVA in all 10 patients, as well as an enhancement to the quality of life in the postoperative period when compared to the preoperative period.
DOI: 10.6084/m9.figshare.8128112.v1
title: Results of Scharioth macular lens implant in patients with age-related macular degeneration

description: Abstract Objective: To describe the outcomes in terms of near visual acuity and quality of life in 10 patients submitted to Scharioth Macular Lens implantation. Setting: João Eugênio Eye Institute, Brasilia, Brazil. Design: Uncontroled clinical trial. Methods: Ten pseudophakic patients with DMRI were submitted to the implantation of intraocular SML in the dominant eye in the period of December of 2017 to the March of 2018. All the patients had answered the Low-Vision Quality of Life Questionaire (LVQOLQ) before and after the surgery, with interval of 30 days. Results: The results points showed a statistical significant improvement (p&lt;0.001) in the near visual acuity (NVA), without interference in the distance vision. All patients had significant improvements (p&lt;0,05) in LVQOLQ on the item that evaluates the near visual acuity. Conclusion: There was an improvement of NVA in all 10 patients, as well as an enhancement to the quality of life in the postoperative period when compared to the preoperative period.