cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647996
title: CIL:34904

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647996.v1
title: CIL:34904

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647997.v1
title: CIL:34905

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647998.v1
title: CIL:34906

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647999.v1
title: CIL:34907

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647994
title: CIL:34902

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647992
title: CIL:34522

description: The video is a representative movie of bacteria incubated in DMEM with BSA for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34521 and 34523, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the transient pulls and the fact that the two bacteria are not as tightly connected to the pillars in this media. This video corresponds to Video 3 from PLoS Biol. 2008 Apr 15;6(4):e87.
DOI: 10.6084/m9.figshare.647995
title: CIL:34903

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647993
title: CIL:34523

description: The video is a representative movie of baceria incubated in DMEM and polylysine for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34521 and 34522, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the absence of motions in this media. This video corresponds to Video 4 from PLoS Biol. 2008 Apr 15;6(4):e87.
DOI: 10.6084/m9.figshare.647994.v1
title: CIL:34902

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647995.v1
title: CIL:34903

description: Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.
DOI: 10.6084/m9.figshare.647990
title: CIL:28781

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in which KNL-2 has been depleted by RNAi. GFP-CeCENP-A was imaged (strain OD101). Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28780), no discrete GFP foci were seen at any point. The video is ∼12 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the left, and GFP is shown on the right.
DOI: 10.6084/m9.figshare.647991
title: CIL:34521

description: The video is a representative movie of baceria incubated in DMEM for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34522 and 34523, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the already deflected pillars around the bacterium and both transient and sustained pulls. This video corresponds to Video 2 from PLoS Biol. 2008 Apr 15;6(4):e87.
DOI: 10.6084/m9.figshare.647993.v1
title: CIL:34523

description: The video is a representative movie of baceria incubated in DMEM and polylysine for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34521 and 34522, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the absence of motions in this media. This video corresponds to Video 4 from PLoS Biol. 2008 Apr 15;6(4):e87.
DOI: 10.6084/m9.figshare.647988
title: CIL:28777

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of KNL-2 by RNAi. GFP-histone and GFP-γ-tubulin were imaged (strain TH32). Pronuclear migration is slightly disrupted by a chromatin bridge resulting from failed meiotic segregation of the maternal chromatin. As seen in CeCENP-A depletion (CIL 28776), chromosome condensation is disrupted, chromosomes fail to form a proper metaphase plate, and the spindle poles move away from the chromatin masses prematurely. Anaphase segregation fails, and chromatin masses are left in the middle of the cell. Also note the meiotic defect that results in a defective oocyte pronucleus (left side of image at the beginning of the videos; this is the embryo anterior). The video is ∼16 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.
DOI: 10.6084/m9.figshare.647989
title: CIL:28779

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi in strain OD31. Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28778), no discrete GFP foci were seen until anaphase onset, at which point foci of GFP-KNL-2 formed along the spindle and on the chromatin. The video is ∼18 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.
DOI: 10.6084/m9.figshare.647989.v1
title: CIL:28779

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi in strain OD31. Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28778), no discrete GFP foci were seen until anaphase onset, at which point foci of GFP-KNL-2 formed along the spindle and on the chromatin. The video is ∼18 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.
DOI: 10.6084/m9.figshare.647991.v1
title: CIL:34521

description: The video is a representative movie of baceria incubated in DMEM for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34522 and 34523, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the already deflected pillars around the bacterium and both transient and sustained pulls. This video corresponds to Video 2 from PLoS Biol. 2008 Apr 15;6(4):e87.
DOI: 10.6084/m9.figshare.647987
title: CIL:25703

description: MTLn3 cell cotransfected with control siRNA and two tandem Src SH2 phosphotyrosine-binding domains (YFP-dSH2), which specifically detects tyrosine phosphorylation at focal adhesions. CIL:25702 is corresponds to FAK siRNA (target A). Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Movie corresponds to video 4 from J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13.
DOI: 10.6084/m9.figshare.647990.v1
title: CIL:28781

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in which KNL-2 has been depleted by RNAi. GFP-CeCENP-A was imaged (strain OD101). Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28780), no discrete GFP foci were seen at any point. The video is ∼12 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the left, and GFP is shown on the right.
DOI: 10.6084/m9.figshare.647986
title: CIL:25702

description: MTLn3 cell cotransfected with FAK siRNA (target A) and two tandem Src SH2 phosphotyrosine-binding domains (YFP-dSH2), which specifically detects tyrosine phosphorylation at focal adhesions. CIL:25703 is the corresponding control experiment. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Movie corresponds to video 5 from J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13.
DOI: 10.6084/m9.figshare.647987.v1
title: CIL:25703

description: MTLn3 cell cotransfected with control siRNA and two tandem Src SH2 phosphotyrosine-binding domains (YFP-dSH2), which specifically detects tyrosine phosphorylation at focal adhesions. CIL:25702 is corresponds to FAK siRNA (target A). Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Movie corresponds to video 4 from J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13.
DOI: 10.6084/m9.figshare.647988.v1
title: CIL:28777

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of KNL-2 by RNAi. GFP-histone and GFP-γ-tubulin were imaged (strain TH32). Pronuclear migration is slightly disrupted by a chromatin bridge resulting from failed meiotic segregation of the maternal chromatin. As seen in CeCENP-A depletion (CIL 28776), chromosome condensation is disrupted, chromosomes fail to form a proper metaphase plate, and the spindle poles move away from the chromatin masses prematurely. Anaphase segregation fails, and chromatin masses are left in the middle of the cell. Also note the meiotic defect that results in a defective oocyte pronucleus (left side of image at the beginning of the videos; this is the embryo anterior). The video is ∼16 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.
DOI: 10.6084/m9.figshare.647984
title: CIL:24805

description: Differential response of lamellipodial actin network to latrunculin a (LA). Electron micrograph of a Xenopus fibroblast lamellipodium treated with LA (0.25 μM for 10 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated keratocyte is available as CIL 24804. Image corresponds to Figure 7e from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647985
title: CIL:24806

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes. Fluorescence microscopy of a whole cell double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC in lamellipodium is excluded from the narrow zone at the extreme leading edge. Immuno-EM with localization of XAC is shown in CIL 24807 and 24808. Image corresponds to Figure 8a-c from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647986.v1
title: CIL:25702

description: MTLn3 cell cotransfected with FAK siRNA (target A) and two tandem Src SH2 phosphotyrosine-binding domains (YFP-dSH2), which specifically detects tyrosine phosphorylation at focal adhesions. CIL:25703 is the corresponding control experiment. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Movie corresponds to video 5 from J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13.
DOI: 10.6084/m9.figshare.647982
title: CIL:24803

description: Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblast after unprotected extraction without polyethelene glycol and phalloidin. Actin network at lamellipodial rear disassembled in the course of unprotected extraction, whereas front zone remained as dense as in control cells (CIL 24801). Image corresponds to Figure 6f from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647982.v1
title: CIL:24803

description: Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblast after unprotected extraction without polyethelene glycol and phalloidin. Actin network at lamellipodial rear disassembled in the course of unprotected extraction, whereas front zone remained as dense as in control cells (CIL 24801). Image corresponds to Figure 6f from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647983
title: CIL:24804

description: Differential response of lamellipodial actin network to latrunculin a (LA), a binder of monomeric actin. This electron micrograph of a Xenopus keratocyte)lamellipodium treated with LA (0.1 μM for 30 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated fibroblast is available as CIL 24805. Image corresponds to Figure 7d from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647984.v1
title: CIL:24805

description: Differential response of lamellipodial actin network to latrunculin a (LA). Electron micrograph of a Xenopus fibroblast lamellipodium treated with LA (0.25 μM for 10 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated keratocyte is available as CIL 24804. Image corresponds to Figure 7e from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647985.v1
title: CIL:24806

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes. Fluorescence microscopy of a whole cell double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC in lamellipodium is excluded from the narrow zone at the extreme leading edge. Immuno-EM with localization of XAC is shown in CIL 24807 and 24808. Image corresponds to Figure 8a-c from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647980
title: CIL:24801

description: Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblasts after regular extraction in the presence of polyethelene glycol (PEG) and phalloidin. While the actin network in the front zone of these cels remains dense, the actin network at the lamellipodial rear disassembled in the course of unprotected extraction (CIL 24800). Image corresponds to Figure 6c from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647981
title: CIL:24802

description: Structural differentiation of actin network in lamellipodium. Electon micrograph Xenopus fibroblast after unprotected extraction without polyethelene glycol. Actin network at lamellipodial rear disassembled in the course of unprotected extraction, whereas front zone remained as dense as in control cells (CIL 24801). Image corresponds to Figure 6e from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647983.v1
title: CIL:24804

description: Differential response of lamellipodial actin network to latrunculin a (LA), a binder of monomeric actin. This electron micrograph of a Xenopus keratocyte)lamellipodium treated with LA (0.1 μM for 30 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated fibroblast is available as CIL 24805. Image corresponds to Figure 7d from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647980.v1
title: CIL:24801

description: Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblasts after regular extraction in the presence of polyethelene glycol (PEG) and phalloidin. While the actin network in the front zone of these cels remains dense, the actin network at the lamellipodial rear disassembled in the course of unprotected extraction (CIL 24800). Image corresponds to Figure 6c from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647981.v1
title: CIL:24802

description: Structural differentiation of actin network in lamellipodium. Electon micrograph Xenopus fibroblast after unprotected extraction without polyethelene glycol. Actin network at lamellipodial rear disassembled in the course of unprotected extraction, whereas front zone remained as dense as in control cells (CIL 24801). Image corresponds to Figure 6e from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647976
title: CIL:24580

description: A single frame from the movie (CIL:20154) that shows a large rounded monkey kidney cell being penetrated by a pseudopodia followed by the whole Balamuthia ameba. Detection of the ameba within, rather than on, or beneath the cell is indicated by the change in clarity of the ameba outside and then inside the cell's exterior membrane. The ameba with splayed pseudopodia had begun to enter in apparently a slit in the surface of the cell. The integrity of the cell is maintained and its size does not change appreciably. The portion of the ameba within the cell is less clear than that portion still outside, and as it continues movement within the cell that continues until only a small portion (tail) of the ameba remains outside. Photographed at 1200X. Additional photographs (grouped with this movie) show the movement of the ameba inside of the cell its movement deforms the rounded exterior membrane before the whole ameba emerges some 20 minutes later.
DOI: 10.6084/m9.figshare.647978
title: CIL:24785

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus fibroblasts. Immuno-EM with XAC antibody at high magnification. Low magnification view is available at CIL 24784. Nucleus and surrounding regions look bright because of high content of cellular proteins, which creates high electron density (brightness in inverted contrast). Immunogold-labeling in these central regions is very low. Image corresponds to Figure 10i from J Cell Biol. 1999 May 31;145(5):1009-26.