DOI: 10.48436/4ss5q-r8p58
title: Research data for "Highlighting the influence between physical and chemical crosslinking in dynamic hydrogels for two-photon micropatterning"

description: Context

This dataset was created from original work conducted in the framework of a PhD project and compiled in a publication ("Highlighting the Influence between Physical and Chemical Cross-Linking in Dynamic Hydrogels for Two-Photon Micropatterning", https://doi.org/10.1021/acs.biomac.5c00062). It provides the raw data of results presented and discussed therein.

Technical details

Microsoft Excel file “Raw Data_Photocleavable Hydrogels”:

Tab 1 - Raw Data obtained for real-time (RT) NIR photorheology of 5-10 wt% PVA-NB:SS-DT hydrogels

Tab 2 - Raw Data obtained for real-time (RT) NIR photorheology of 10 wt% PVA-NB:SS-DT hydrogels with varying Li-TPO content

Tab 3 - Raw Data obtained for real-time (RT) NIR photorheology of IPN5, IPN7.5 and IPN10

Tab 4 - Raw Data obtained for real-time (RT) NIR photorheology of hybrid gels containing 5-10 wt% PVA-NB:SS-DT and 10 wt% Gel-NB:SS-DT

Tab 5 - Mass swelling ratio of hydrogels

Tab 6 – Hydrolytic degradation of IPNs

Tab 7 – Hydrolytic degradation of hybrid gels

Tab 8 - Raw Data obtained for real-time (RT) NIR photorheology of IPN5 with varying irradiation times

Tab 9 - Raw Data obtained for real-time (RT) NIR photorheology of IPN5 with varying light sources

Tab 10 - Hydrolytic degradation + mass swell ratio of IPNs

Tab 11 - Raw Data obtained for real-time (RT) NIR photorheology of 10 wt% Gel-NB:SS-DT

Tab 12 - Hydrolytic degradation + mass swell ratio of hybrid gels

NMR zip file

‘1H SS-DT’, ‘13C SS-DT', ‘1H PVA-NB’ and ‘1H Gel-NB’ folders:

NMR data obtained during all synthesis steps of the dithiol crosslinker (SS-DT) and norbornene-modified macromers (PVA-NB, Gel-NB) used herein. See Supplementary Information Chapter 1 for structures and calculation of degree of substitution (DS).

A software to display NMR-spectra is needed, such as MestreNova or Topspin.

Compund descriptions

Compound descriptions in the files included herein adhere to the naming in the related publication referenced in the Related Works section, where all compounds are described in detail and drawn as structural formulas. In brief:

PVA-NB: norbornene-modified poly(vinyl alcohol)

Gel-NB: norbornene-modified gelatin (type A, bloom 300)

SS-DT: 2-[2-(3-Mercaptopropionoxy)ethyldithio]ethyl 3-mercaptopropionate

Li-TPO: Lithium-Phenyl-2,4,6-trimethylbenzoylphosphinat
description: Photolabile hydrogels have gained tremendous interest for a wide range of applications in materials and life sciences. Usually, photodegradability is introduced via chromophores and labile bonds, making such materials intrinsically light sensitive. In recent years, disulfide bonds have emerged as an innovative alternative, as they can be selectively cleaved in the presence of (photo)generated radicals. However, such materials suffer from limited network stability and high swelling as a result of thiol–disulfide metathesis reactions. Herein, we present two strategies to counteract such phenomena by network stabilization either via physical or chemical incorporation of (un)modified gelatin macromers to norbornene-modified poly(vinyl alcohol) networks. Photolabile behavior was introduced by a simple disulfide-containing dithiol cross-linker. Tunable material properties were investigated by means of in situ photorheology, in vitro swelling, and degradation experiments. Finally, we demonstrate an innovative method for localized disulfide cleavage via two-photon micropatterning.