DOI: 10.5061/dryad.v41ns1s5b
title: Data from: (Sub-)Antarctic endemic cyanobacteria from benthic mats are rare and have restricted geographic distributions

description: The Antarctic terrestrial macrobiota are highly endemic and
 biogeographically structured, but whether this also holds true for
 microbial groups remains poorly understood. We studied the biogeographic
 patterns of Antarctic cyanobacteria from benthic microbial mats sampled in
 84 lakes from two sub-Antarctic islands, as well as from eight Antarctic
 Conservation Biogeographic Regions (ACBRs) which were previously defined
 based mainly on macroscopic taxa. Analysis of 16S rRNA gene sequences
 revealed that Antarctic and sub-Antarctic lakes host significantly
 different cyanobacterial communities, yet that the bioregionalization
 pattern did not correspond to the division into ACBRs. Both Antarctic and
 sub-Antarctic lakes contain a high number of potentially endemic taxa (41%
 of the total diversity), of which 33.3% attain a relative abundance of
 &lt; 1%. Our findings highlight the uniqueness of Antarctic microbiota
 and the need for increased protection of inland waters in both Antarctica
 and the sub-Antarctic islands.
description: Benthic microbial mats sampled were collected on 84 lakes from
 2 sub-Antarctic islands and 8 Antarctic Conservation Biogeographic Regions
 (ACBRs). For each samples, partial 16s rRNA gene
 were sequenced using Illumina MiSeq plateform.
description: # (Sub-)Antarctic endemic cyanobacteria from benthic mats are rare and
 have restricted geographic distributions
 [https://doi.org/10.5061/dryad.v41ns1s5b](https://doi.org/10.5061/dryad.v41ns1s5b) ## Description of the data and file structure The microbial mats were sampled in 84 lakes or ponds across 8 ACBRs from Antarctica (n = 75) and 2 sub-Antarctic islands (n=14). Partial 16s rRNA genes were then sequenced using Illumina MiSeq platform. The paired-end reads were merged and filtered (--maxdiffs 10, --maxdiffpct 8), and reads that did not contain primer and barcodes were removed. ### Files and variables #### File: DRMAU\_SC2\_litt.fastq **Description:** ACBR = Dronning Maud Land, Lake = SC2 #### File: DRMAU\_SC5\_litt.fastq **Description:** ACBR = Dronning Maud Land, Lake = SC5 #### File: DRMAU\_SC1\_litt.fastq **Description:** ACBR = Dronning Maud Land, Lake = SC1 #### File: DRMAU\_SC6\_litt.fastq **Description:** ACBR = Dronning Maud Land, Lake = SC6 #### File: DRMAU\_SC7\_litt.fastq **Description:** ACBR = Dronning Maud Land, Lake = SC7 #### File: EDRBY\_EO7.fastq **Description:** ACBR = Enderby Land, Lake = EO7 #### File: EDRBY\_LA7\_litt\_0.1A.fastq **Description:** ACBR = Enderby Land, Lake = LA7 #### File: EDRBY\_Lake\_Barkell.fastq **Description:** ACBR = Enderby Land, Lake = Lake Barkell #### File: EDRBY\_L\_Lorna\_H2.fastq **Description:**  ACBR = Enderby Land, Lake = Lorna #### File: EDRBY\_SK1\_G0-1\_Mago\_Ike\_L.fastq **Description:**  ACBR = Enderby Land, Lake = Mago Ike lake #### File: EDRBY\_SK5\_surface.fastq **Description:**  ACBR = Enderby Land, Lake = SK5 - Naka-Tempyo Ike lake #### File: EDRBY\_SK5.fastq **Description:**  ACBR = Enderby Land, Lake = SK5 - Naka-Tempyo Ike lake #### File: EDRBY\_Ufs\_Isl\_ID2\_6\_Hemsworth\_I.fastq **Description:**  ACBR = Enderby Land, Lake = Ufs island lake #### File: EDRBY\_WO1\_litt.fastq **Description:**  ACBR = Enderby Land, Lake = W01 #### File: EDRBY\_WO4\_litt\_0.1m.fastq **Description:**  ACBR = Enderby Land, Lake = W04 #### File: EDRBY\_WO6\_litt\_0.1m.fastq **Description:**  ACBR = Enderby Land, Lake = W06 #### File: EDRBY\_WO8\_litt\_epips\_0.2m.fastq **Description:**  ACBR = Enderby Land, Lake = W08 #### File: EDRBY\_WO6\_litt\_1.5m\_east\_side.fastq **Description:**  ACBR = Enderby Land, Lake = W06 #### File: ESANT\_Ambio\_14.fastq **Description:**  ACBR = East Antarctica, Lake =Fold lake #### File: EDRBY\_WO8\_litt.fastq **Description:**  ACBR = Enderby Land, Lake =WO8 #### File: ESANT\_Ambio\_19.fastq **Description:**  ACBR = East Antarctica, Lake = Lake Burgess #### File: ESANT\_Ambio\_24.fastq **Description:**  ACBR = East Antarctica, Lake = Ace lake #### File: ESANT\_Highway\_VH26.fastq **Description:**  ACBR = East Antarctica, Lake = Highway lake #### File: ESANT\_Corner\_L\_litt\_.fastq **Description:**  ACBR = East Antarctica, Lake = Corner lake 1 #### File: ESANT\_L\_Alanna.fastq **Description:**  ACBR = East Antarctica, Lake = lake Alanna #### File: ESANT\_L\_Jack\_.fastq **Description:**  ACBR = East Antarctica, Lake = Lake Jack #### File: ESANT\_M5\_Bunger\_Hills.fastq **Description:**  ACBR = East Antarctica, Lake = M5 #### File: ESANT\_M15\_Bunger\_Hills.fastq **Description:**  ACBR = East Antarctica, Lake = M15 #### File: ESANT\_Organic\_L.fastq **Description:**  ACBR = East Antarctica, Lake = Organic lake #### File: ESANT\_M16\_Bunger\_Hills.fastq **Description:** ACBR = East Antarctica, Lake = M16 #### File: ESANT\_Pendant\_L.fastq **Description:** ACBR = East Antarctica, Lake = Pendant lake #### File: ESPEN\_L17FR.fastq **Description:** ACBR = North East Antarctic Peninsula, Lake = L17 #### File: ESANT\_Rauer2\_RI2.fastq **Description:** ACBR = East Antarctica, Lake = Rauer 2 #### File: ESANT\_Waterfall\_L.fastq **Description:** ACBR = East Antarctica, Lake = Waterfall Lake #### File: ESANT\_Reid\_litt.fastq **Description:** ACBR = East Antarctica, Lake = Lake Reid #### File: ESANT\_Watts\_L.fastq **Description:** ACBR = East Antarctica, Lake = Watts Lake #### File: ESPEN\_L18FR.fastq **Description:** ACBR = North East Antarctic Peninsula, Lake = L18 #### File: ESPEN\_L19FR.fastq **Description:** ACBR = North East Antarctic Peninsula, Lake = L19 #### File: ESPEN\_L30FR.fastq **Description:** ACBR = North East Antarctic Peninsula, Lake = L30 #### File: ESPEN\_L32FR.fastq **Description:** ACBR = North East Antarctic Peninsula, Lake = L32FR #### File: INDIA\_MI02.fastq **Description: **Island = Marion Isand, Lake = MI02 #### File: INDIA\_MI3.fastq **Description:** Island = Marion Isand, Lake = MI3 #### File: INDIA\_MI4.fastq **Description:** Island = Marion Isand, Lake = MI4 #### File: INDIA\_MI16.fastq **Description:** Island = Marion Isand, Lake = MI16 #### File: INDIA\_MI20.fastq **Description:** Island = Marion Isand, Lake = MI20 #### File: INDIA\_MI44.fastq **Description:** Island = Marion Isand, Lake = MI44 #### File: INDIA\_MI52.fastq **Description:** Island = Marion Isand, Lake = MI52 #### File: INDIA\_MI44\_dupl.fastq **Description:** Island = Marion Isand, Lake = MI44 (duplicate sample) #### File: INDIA\_MI60.fastq **Description:** Island = Marion Isand, Lake = MI60 #### File: NVICT\_6-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake 14 #### File: NVICT\_06-12.fastq **Description:** ACBR = North Victoria Land, Lake = Lake 15 #### File: NVICT\_70-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake 20A #### File: NVICT\_06-51.fastq **Description:** ACBR = North Victoria Land, Lake = Lake 7 #### File: NVICT\_26-06.fastq **Description:** ACBR = North Victoria Land, Lake =Lake Gondwana Base Lake #### File: NVICT\_36-06.fastq **Description:** ACBR = North Victoria Land, Lake = Laguna Salmastra #### File: NVICT\_178-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake 15A #### File: NVICT\_37-XX.fastq **Description:** ACBR = North Victoria Land, Lake = Lake #### File: NVICT\_211-XIX.fastq **Description:** ACBR = North Victoria Land, Lake = Lake D (4 - vicino mare) #### File: NVICT\_153-06.fastq **Description:** ACBR = North Victoria Land, Lake = Carezza lake #### File: NVICT\_21-06.fastq **Description:** ACBR = North Victoria Land, Lake =Lake Gondwana (alto) #### File: NVICT\_147-06.fastq **Description:** ACBR = North Victoria Land, Lake = Skua lake #### File: NVICT\_161-XX.fastq **Description:** ACBR = North Victoria Land, Lake = Enigma lake #### File: NVICT\_240-06\_dupl.fastq **Description:** ACBR = North Victoria Land, Lake = Lake (duplicate sample) #### File: NVICT\_170-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake 3 #### File: NVICT\_161-06.fastq **Description:** ACBR = North Victoria Land, Lake = Ponds #### File: NVICT\_230-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake North #### File: NVICT\_183-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake #### File: NVICT\_240-06.fastq **Description:** ACBR = North Victoria Land, Lake = Lake #### File: NVICT\_251-XX.fastq **Description:** ACBR = North Victoria Land, Lake = Lake #### File: PACIF\_MAC2.fastq **Description:** Island = Macquarie Island, Lake = MAC2 #### File: PACIF\_MAC4.fastq **Description:** Island = Macquarie Island, Lake = MAC4 #### File: PACIF\_MAC6.fastq **Description:** Island = Macquarie Island, Lake = MAC6 #### File: PACIF\_Square\_L.fastq **Description:** Island = Macquarie Island, Lake = Square Lake #### File: PACIF\_MAC10.fastq **Description:** Island = Macquarie Island, Lake = MAC10 #### File: PACIF\_Tulloch\_Lake.fastq **Description:** Island = Macquarie Island, Lake = Tulloch lake #### File: SVICT\_06-265\_dupl.fastq **Description:** ACBR = South Victoria Land, Lake = Pond (duplicate sample) #### File: SVICT\_06-265.fastq **Description:** ACBR = South Victoria Land, Lake = Pond #### File: SVICT\_69-06.fastq **Description:** ACBR = South Victoria Land, Lake = Lake 20 #### File: SVICT\_227-XX.fastq **Description:** ACBR = South Victoria Land, Lake = Lake E (5) #### File: SVICT\_254-06.fastq **Description:** ACBR = South Victoria Land, Lake = Lake G #### File: SVICT\_278-06.fastq **Description:** ACBR = South Victoria Land, Lake = Pond #### File: SVICT\_282-06.fastq **Description:** ACBR = South Victoria Land, Lake =Oasi Lake #### File: SVICT\_278-06\_dupl.fastq **Description:** ACBR = South Victoria Land, Lake = Pond (duplicate sample) #### File: SVICT\_AMB37.fastq **Description:** ACBR = South Victoria Land, Lake = Hoare_L \_SS #### File: SVICT\_L\_Fryxell.fastq **Description:** ACBR = South Victoria Land, Lake = Lake Fryxell #### File: SVICT\_FRY2.fastq **Description:** ACBR = South Victoria Land, Lake = Lake Fryxell #### File: SVICT\_L\_Hoare\_dupl.fastq **Description:** ACBR = South Victoria Land, Lake = Lake Hoare (duplicate sample) #### File: TRMNT\_Lundstrom\_TM4.fastq **Description:** ACBR = Transantantarctic Mountains, Lake = Lundström Lake #### File: TRMNT\_Forlidas\_TM2.fastq **Description:** ACBR = Transantantarctic Mountains, Lake = Forlidas Pond #### File: SVICT\_L\_Hoare.fastq **Description:** ACBR = South Victoria Land, Lake = Lake Hoare #### File: WSPEN\_Col1\_L.fastq **Description:** ACBR = North West Antarctic Peninsula, Lake = Col Lake 1 #### File: TRMNT\_Mount\_Lowry.fastq **Description:** ACBR = Transantantarctic Mountains, Lake = Lake Moat #### File: WSPEN\_PP\_mat.fastq **Description:** ACBR = North West Antarctic Peninsula, Lake = Narrows Lake #### File: WSPEN\_PP\_Shallow\.fastq **Description:** ACBR = North West Antarctic Peninsula, Lake = Narrows Lake ## Access information Other publicly accessible locations of the data: * https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA1107504