cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/z99nv-wv929
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter1 558 lysed/PVP
description: <strong>Tilt Series Date:</strong> 2013-10-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> -     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xgBuffer 1:10% Polyvinylpyrrolidone (PVP)1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-04-7/rawdata/</p>Rhodopvp0007.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-04-7/rawdata/Rhodopvp0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp0007.rec,  Reconstruction (Pixel Size 0.64 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-04-7/3dimage_31877/Rhodopvp0007.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v9ejf-p8g71
title: Hylemonella + Bdellovibrio

description: Raw data files of Hylemonella + Bdellovibrio
description: <strong>Tilt Series Date:</strong> 2013-01-03</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Hylemonella grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, incubated with attack-phase Bdellovibrio for 3 days, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2013-01-03-17_slicer5732.jpg: chemoreceptor ycw2013-01-03-17_slicer5733.jpg: filaments ycw2013-01-03-17_slicer5735.jpg: membrane irregularity ycw2013-01-03-17_slicer5743.jpg: flagellar motor? .  chemoreceptor connection?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-17/rawdata/</p>Bdel_Hyle_0020005.mrc,  Tilt Series (Pixel Size 0.4 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-17/rawdata/Bdel_Hyle_0020005.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdel_Hyle_0020005_full.rec,  Reconstruction (Pixel Size 1.6 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-17/3dimage_24285/Bdel_Hyle_0020005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7qvc5-r6p43
title: Caulobacter crescentus

description: wild type CB15N. 1 of 1, slight warping. lightly fixed cells; no filaments seen
description: <strong>Tilt Series Date:</strong> 2006-04-04</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.75°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; exponential phase; glutaraldehyde-fixed on grid; see DPD notebook Feb 2-3, 2006</p>
<strong>Sample Preparation:</strong> box 177, grid 4; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1 sec, drain 1.5 sec; see DPD notebook Aug 14, 2006</p>
  SAVE_CC06Apr04t01_xy.jpg: file associated to 3D Image #289 pdd2006-04-04-1_slicer10031.jpg: Caulobacter crescentus .  s-layer pdd2006-04-04-1_slicer10032.jpg: Caulobacter crescentus .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-04-1/rawdata/</p>cc06apr04t01.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-04-1/rawdata/cc06apr04t01.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC06Apr04t01bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-04-1/3dimage_289/CC06Apr04t01bn_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/aghhm-gys90
title: S. pombe (Cdc25-22)

description: Cryosections of S. pombe (Cdc25-22) synchronized for actomyosin ring formation.
description: <strong>Tilt Series Date:</strong> 2013-10-05</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mohan Balasubramanian and Mithilesh Mishra</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown to OD = 0.2 and them temperature shifted to the nonpermissive temp of 36C for 4 hours. Then cells were shifted to the permissive temp of 25C and monitored by fLM for ring formation (between 30-60 min). ~90 are synchronized.</p>
<strong>Sample Preparation:</strong> After synchronization cells were pelleted, mixed to include ~ 20% dextran and high pressure frozen. They were then cryosectioned at 150-200 nm.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-10-05-7/rawdata/</p>100513_pombe0007.mrc,  Tilt Series (Pixel Size 0.616 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-10-05-7/rawdata/100513_pombe0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        100513_pombe0007.rec,  Reconstruction (Pixel Size 2.464 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-10-05-7/3dimage_44120/100513_pombe0007.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rj00j-mks82
title: S. pombe (Cdc25-22)

description: Cryosections of S. pombe (Cdc25-22) synchronized for actomyosin ring formation.
description: <strong>Tilt Series Date:</strong> 2013-10-11</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mohan Balasubramanian and Mithilesh Mishra</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown to OD = 0.2 and them temperature shifted to the nonpermissive temp of 36C for 4 hours. Then cells were shifted to the permissive temp of 25C and monitored by fLM for ring formation (between 30-60 min). ~90 are synchronized.</p>
<strong>Sample Preparation:</strong> After synchronization cells were pelleted, mixed to include ~ 20% dextran and high pressure frozen. They were then cryosectioned at 150-200 nm.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-10-11-1/rawdata/</p>101113_pombe0001.mrc,  Tilt Series (Pixel Size 0.616 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-10-11-1/rawdata/101113_pombe0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        101113_pombe0001.rec,  Reconstruction (Pixel Size 2.464 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-10-11-1/3dimage_44094/101113_pombe0001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ecy2n-04d33
title: H. pylori 26695 / Hs.746T

description: Grid box YWC057
description: <strong>Tilt Series Date:</strong> 2013-12-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-24/rawdata/</p>20131216_YWC057_10018.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-24/rawdata/20131216_YWC057_10018.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131216_YWC057_10018_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-24/3dimage_36154/20131216_YWC057_10018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dptq7-xt684
title: H. pylori 26695 / Hs.746T

description: Grid box YWC046
description: <strong>Tilt Series Date:</strong> 2013-11-05</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4.Hs.746T cells cultivated on QF Au finder grid for 1 day. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (100 ul gold resuspent in 30 ul). Manual blot by Whatman 1 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-05-17/rawdata/</p>20131104_YWC046_30001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-05-17/rawdata/20131104_YWC046_30001.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131104_YWC046_30001_part121_10.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-05-17/3dimage_33974/20131104_YWC046_30001_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/srz95-em376
title: Vibrio cholerae MKW1383

description: Vibrio cholerae MKW1383 in the mixture with Bdellovibrio.Imaged by Janelia farm Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2013-05-01-18_slicer9830.jpg: Vibrio cholerae  .  chemoreceptor array
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-18/rawdata/</p>grid3-tiltseries0018.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-18/rawdata/grid3-tiltseries0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        grid3-tiltseries0018_full.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-18/3dimage_26417/grid3-tiltseries0018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6ntw3-xnb14
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-08-21_slicer3779.jpg: Myxococcus xanthus. Another example of vesicles in a row. ycw2014-03-08-21_slicer7949.jpg: filament bundle, Myxococcus xanthus ycw2014-03-08-21_slicer7951.jpg: Myxococcus xanthus, T6SS ycw2014-03-08-21_slicer7953.jpg: unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-21/rawdata/</p>20140308_YWC093_10034.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-21/rawdata/20140308_YWC093_10034.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140308_YWC093_10034_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-21/3dimage_40035/20140308_YWC093_10034_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yacxv-bjg70
title: S. pombe (Cdc25-22)

description: Cryosections (180 nm nominal thickness)
description: <strong>Tilt Series Date:</strong> 2014-04-19</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized cytokinesis. From OD 600 = 0.25 grow for 4 hrs at 36 C. Return to 25 C for 45 min then begin monitoring ring formation by LM. Once 80-90 of rings are continuous they were mixed with Dextran (100 ul of 40% Dextran in ~ 400 ul of pelleted cells). They were then high pressure frozen.</p>
<strong>Sample Preparation:</strong> High pressure freezing followed by cryosectioning</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-25/3dimage_42084/</p>041914_pombe_0025.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-25/3dimage_42084/041914_pombe_0025.rec"
        > <i class="download icon"></i> Download </a></p>

        041914_pombe_0025.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-25/rawdata/041914_pombe_0025.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c7me9-97062
title: Myxococcus xanthus delta-pilP

description: grid box YWC064
description: <strong>Tilt Series Date:</strong> 2013-12-30</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2013-12-30-28_slicer8957.jpg: Filament bundle .  Myxococcus xanthus ycw2013-12-30-28_slicer8959.jpg: Type 6 secretion system .  T6SS .  Myxococcus xanthus ycw2013-12-30-28_slicer10548.jpg: Myxococcus xanthus contracted T6SS
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-28/rawdata/</p>20131230_YWC064_10028.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
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        20131230_YWC064_10028_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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