DOI: 10.5061/dryad.r2280gbs6
title: Data from: Electrochemical sensing of hormonal serotonin levels in Crayfish

description: The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been
 implicated as a key biomarker that regulates a range of neurobehavioral
 functions. 5-HT also plays a prominent role in modulating social behavior
 across species. Here, we report the successful differentiation of hormonal
 (circulatory) 5-HT concentrations in socially isolated and communal
 crayfish samples by a surface-modified carbon fiber microelectrode
 electrochemical sensor. Monitoring 5-HT concentrations across social
 conditions provided important insights into mechanisms linking isolation
 to neurohormones that guide behavior. Utilizing surface coatings and
 electrochemical etching, our sensor can detect endogenous, nanomolar
 concentrations of 5-HT in vitro from crayfish hemolymph (i.e., blood)
 collected from communally housed animals and those isolated for one or
 seven days. Our measurements revealed that crayfish responses to isolation
 varied by species: Procambarus crayfish isolated for seven days exhibited
 a major increase in 5-HT compared to communally housed or short-term
 isolated conspecifics, whereas for Faxonius crayfish, the hormonal
 concentrations of 5-HT peaked after one day of isolation but paralleled
 the levels of communally housed crayfish after one week of isolation.
 These findings underscore the complex nature of neurochemical pathways in
 response to social conditions, demonstrate the sensor's utility for
 studying 5-HT dynamics, and provide a method for monitoring temporal
 changes in hormonal 5-HT following social isolation. 
description: All statistical analyses were performed using IBM SPSS (IBM
 Corp.). Ipa data is represented as the mean of measurements from a sample
 (n=3). Samples where no 5-HT was detected (Ipa=0 µA) were excluded from
 statistical analysis. Statistical comparisons among social groups and
 species were completed using a one-wayanalysis of variance (ANOVA).
 Differences between species were analyzed using non-parametric
 Kruskal-Wallis tests, followed by Mann-Whitney U tests for pair-wise
 comparison. Single-variable cross-species groups were evaluated using
 Mann-Whitney U tests. Statistical significance was defined at
 p&lt;0.05.
description: # Data from: Electrochemical sensing of hormonal serotonin levels in
 Crayfish Dataset DOI:
 [10.5061/dryad.r2280gbs6](https://doi.org/10.5061/dryad.r2280gbs6) ##
 Description of the data and file structure The excel sheet consists of raw
 data including the 5-HT concentration, Ipa values, weight, sex, label,
 social condition, and feeding conditions. The excel sheet lists all the
 values compiled and sorted for easier access. The first three tabs of the
 excel sheet includes the statistical output and input of the dataset,
 including ANOVA test, Kruskal-Wallis, and Mann-Whitney U.  Multiple files
 in this repository require SPSS to generate. The SPSS files contain the
 raw input data for each test as well and statistical outputs.   ### Files
 and variables #### File: Data_IsolateCommunal.xlsx **Description:** Sorted
 raw data sheet including every experimental variable described below.  
 ##### Variables * Data sorted by social condition, weight (in grams), sex,
 Ipa (in µA), 5-HT concentration (in nM), feeding condition, linear
 regression coefficient, codetection calibration (Ipa (in µA) and
 concentration (in nM), and statistical outputs.   * To Calculate
 Concentration from Current Response, use a current response value and a
 linear regression model (A = slope, B = intercept) to compute
 concentration:\ Concentration = (Ipa – B) / A.  * Dates listed as
 mm/dd/yyyy.  * Empty cells in the *ANOVA Statistical Results* tab is due
 to inapplicable values. The cells are filled according to the statistical
 output.  #### File: KW_MWU_Serotonin_Overton.sav **Description:** Input
 data to generate Kruskal-Wallis and Mann-Whitney U statistical outputs for
 species and social conditions. The variables include *Procambarus
 clarkii* serotonin concentration (in nM), *Procambarus clarkii* social
 condition (COMs, denoted by 1, 1-day ISOs, denoted by 2, and 7-day ISOs,
 denoted by 3), *Faxonius* serotonin concentration (in nM),
 and *Faxonius* social condition (COMs, denoted by 1, 1-day ISOs, denoted
 by 2, and 7-day ISOs, denoted by 3).  #### File:
 ANOVA_Serotonin_Overton.sav **Description:** Input data to generate
 Kruskal-Wallis and Mann-Whitney U statistical outputs for species and
 social conditions. The variables include species (*Procambarus clarkii* or
 *Faxonius*), social condition (COMs, 1-day ISOs, 7-day ISOs), and
 serotonin (in nM).  #### File: MWU_Feeding_Sex_Overton.sav
 **Description:** Input data to generate Mann-Whitney U statistical outputs
 for sex and weight differences and feeding conditions. The variables
 include weight (in grams), sex (females, denoted by 1, and males, denoted
 by 2), serotonin concentration for fed animals (in nM), serotonin
 concentration for all animals (in nM), and species (*Procambarus clarkii*,
 denoted by 1 or *Faxonius,* denoted by 2).  #### File:
 Stats_Output_SPSS_2_Overton.spv **Description:** Statistical data outputs
 for feeding condition as well as the sex and weight differences.  ####
 File: Stats_Output_SPSS_Overton.spv **Description:** Statistical data
 outputs for species and social condition differences.  ## Code/software
 The .sav and .spv files were generated using SPSS software. Use recent
 versions (V31 or earlier) to open the analysis outputs. If access to the
 software if unavailable, .sav files can be opened using, JASP, an
 open-source software. The .spv files can be viewed using an open-source
 software, PSPP.  ## Access information Other publicly accessible locations
 of the data: * N/A Data was derived from the following sources: * N/A