cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/z1mkm-c1a04
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-12-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-2/rawdata/</p>11dec22_C104_1_AT2BT1_0021.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-2/rawdata/11dec22_C104_1_AT2BT1_0021.st"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT2BT1_0021_full.rec,  Reconstruction (Pixel Size 2.0 nm), 346.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-2/3dimage_68103/11dec22_C104_1_AT2BT1_0021_full.rec"
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        11dec22_C104_1_AT2BT1_0021_full.tiff,  file associated to 3D Image #68103, 924.5 kB  
        
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DOI: 10.22002/192zb-fvp20
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-12-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-3/rawdata/</p>11dec22_C104_1_AT2BT1_0023.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-3/rawdata/11dec22_C104_1_AT2BT1_0023.st"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT2BT1_0023_full.rec,  Reconstruction (Pixel Size 2.0 nm), 412.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-3/3dimage_68105/11dec22_C104_1_AT2BT1_0023_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT2BT1_0023_full.tiff,  file associated to 3D Image #68105, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-3/file_15839/11dec22_C104_1_AT2BT1_0023_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tvvjs-1zp50
title: HeLa siRNA tfx CHMP2A/B infx pNLGFP

description: HeLa cells infected with pNLGFP and 12h post infection cells were transfected with siRNA targeting CHMP2A and CHMP2B. 18h after siRNA tfx, cells were frozen Sample# C85
description: <strong>Tilt Series Date:</strong> 2011-06-27</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP2A and 2B siRNAs provided by Wes Sundquist. pNLGFP proviral construct provided by Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward.  Cells transfected, infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and infected and siRNA transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-27-1/rawdata/</p>11Jun27_173_C85_2_0001.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-27-1/rawdata/11Jun27_173_C85_2_0001.st"
        > <i class="download icon"></i> Download </a></p>

        11Jun27_173_C85_2_0001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 681.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-27-1/3dimage_68374/11Jun27_173_C85_2_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11Jun27_173_C85_2_0001_full.tiff,  file associated to 3D Image #68374, 983.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-27-1/file_15889/11Jun27_173_C85_2_0001_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ky2kr-m5627
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch3_0027_full.jpg: file associated to 3D Image #54371
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-11/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch3_0027.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-11/rawdata/11dec12_cryosec_pnlegfp_grid3_Batch3_0027.st"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch3_0027_full.rec,  Reconstruction (Pixel Size 2.0 nm), 470.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-11/3dimage_54371/11dec12_cryosec_pnlegfp_grid3_Batch3_0027_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch3_0027_full.tiff,  file associated to 3D Image #54371, 1.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-11/file_13103/11dec12_cryosec_pnlegfp_grid3_Batch3_0027_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4f5vh-ghv43
title: AimB depletion control w/ xylose

description: Raw data files of AimB depletion control w/ xylose
description: <strong>Tilt Series Date:</strong> 2009-09-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> The AimB_depletion strain was constructed by John Werner, data collected by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity</p>
  zla2009-09-21-36_slicer10067.jpg: Caulobacter crescentus .  flat vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-36/rawdata/</p>09sep21a_AimBxyl_054.mrc,  Tilt Series (Pixel Size 1.192 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-36/rawdata/09sep21a_AimBxyl_054.mrc"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_054_part.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-36/3dimage_10384/09sep21a_AimBxyl_054_part.rec"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_054.tif,  file associated to 3D Image #10384, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-36/file_2849/09sep21a_AimBxyl_054.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4twhk-dax47
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C123
description: <strong>Tilt Series Date:</strong> 2012-03-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-39/3dimage_69170/</p>12mar28_pnlDN_C123_1_AT7BT1_0009_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-39/3dimage_69170/12mar28_pnlDN_C123_1_AT7BT1_0009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12mar28_pnlDN_C123_1_AT7BT1_0009.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-39/rawdata/12mar28_pnlDN_C123_1_AT7BT1_0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar28_pnlDN_C123_1_AT7BT1_0009_full.tiff,  file associated to 3D Image #69170, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-39/file_16132/12mar28_pnlDN_C123_1_AT7BT1_0009_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ee09v-s5984
title: liposomes + 1uL CdvA

description: tetraether liposomes from Sulfolobus cells + 1uL CdvA
description: <strong>Tilt Series Date:</strong> 2011-08-01</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> tetraether liposomes + CdvA</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 1°, constant angular increment, dosage: 8.0 eV/Å²,  defocus: -5.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> sent by Rachel Samson at Oxford University, purified by Parkson Lee-Gau Chong at Temple University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> heavy vortexing for 10 mins at 80 C</p>
<strong>Sample Preparation:</strong> prepared by Parkson Lee-Gau Chong</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-01-8/rawdata/</p>liposomes1uLcdvA_27500x0011.mrc,  Tilt Series (Pixel Size 7.74 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-01-8/rawdata/liposomes1uLcdvA_27500x0011.mrc"
        > <i class="download icon"></i> Download </a></p>

        liposomes1uLcdvA_27500x0011_full.rec,  Reconstruction (Pixel Size 1.5 nm), 340.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-01-8/3dimage_19407/liposomes1uLcdvA_27500x0011_full.rec"
        > <i class="download icon"></i> Download </a></p>

        liposomes1uLcdvA_27500x0011.rec,  Reconstruction (Pixel Size 1.5 nm), 170.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-01-8/3dimage_19408/liposomes1uLcdvA_27500x0011.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4hn98-f1x16
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-32/3dimage_68637/</p>12apr28_C126_2_pNLDN_COS7_AT7BT1_0005_part82_11.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-32/3dimage_68637/12apr28_C126_2_pNLDN_COS7_AT7BT1_0005_part82_11.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT7BT1_0005.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-32/rawdata/12apr28_C126_2_pNLDN_COS7_AT7BT1_0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT7BT1_0005.tiff,  file associated to 3D Image #68637, 740.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-32/file_15934/12apr28_C126_2_pNLDN_COS7_AT7BT1_0005.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m7vmz-0q788
title: HeLa siRNA tfx CHMP2A/B infx pNLGFP

description: HeLa cells infected with pNLGFP and 12h post infection cells were transfected with siRNA targeting CHMP2A and CHMP2B. 18h after siRNA tfx, cells were frozen Sample# C84-2/3
description: <strong>Tilt Series Date:</strong> 2011-05-31</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP2A and 2B siRNAs provided by Wes Sundquist. pNLGFP proviral construct provided by Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward.  Cells transfected with siRNA and then infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and infected and siRNA transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-4/3dimage_68493/</p>11may31_b7_full.rec,  Reconstruction (Pixel Size 2.0 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-4/3dimage_68493/11may31_b7_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11may31_b7.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-4/rawdata/11may31_b7.st"
        > <i class="download icon"></i> Download </a></p>

        11may31_b7.tiff,  file associated to 3D Image #68493, 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-4/file_15917/11may31_b7.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ckyvv-4kw22
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch2_0003_fid.jpg: file associated to 3D Image #54357
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-4/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch2_0003.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        11dec12_cryosec_pnlegfp_grid3_Batch2_0003_full.rec,  Reconstruction (Pixel Size 2.0 nm), 668.9 MB  
        
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        11dec12_cryosec_pnlegfp_grid3_Batch2_0003_fid.tiff,  file associated to 3D Image #54357, 1.2 MB  
        
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