cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25838/d5p-59
title: Petersilie et al. 2024 - Figure 3
title: Cortical brain organoid slices (cBOS) as a functional model system to study human neural cells in intact minimal networks

description: Brain organoids derived from human pluripotent stem cells are a promising tool for studying human neurodevelopment and related disorders. Here, we generated long-term cultures of cortical brain organoid slices (cBOS) grown at the air-liquid interphase from regionalized cortical organoids. We show that cBOS host mature neurons and astrocytes organized in complex architecture. Whole-cell patch clamp demonstrated subthreshold synaptic inputs and action potential firing of neurons. Spontaneous intracellular calcium signals turned into synchronous large-scale oscillations upon combined disinhibition of NMDA receptors and blocking of GABAA receptors. Brief metabolic inhibition to mimic transient energy restriction in the ischemic brain induced reversible intracellular calcium loading of cBOS. Moreover, metabolic inhibition induced a reversible decline in neuronal ATP as revealed by ATeam1.03YEMK. Overall, cBOS provide a powerful platform to assess morphological and functional aspects of human neural cells in intact minimal networks and to address the pathways that drive cellular damage during brain ischemia.
DOI: 10.25345/c5m32nm8p
title: MassIVE MSV000093951 - AH metabolites/zinc chelators associated with POAG identified by GC-MS-based metabolomic analysis
title: AH metabolites/zinc chelators associated with POAG identified by GC-MS-based metabolomic analysis. Methanol/chloroform extraction, derivatization - methoxylamine hydrochloride and of N,O-Bis(trimethylsilyl)trifluoroacetamide
DOI: 10.5281/zenodo.10581346
title: Dataset_FCC_UGC
DOI: 10.5281/zenodo.10581347
title: Dataset_FCC_UGC
DOI: 10.25430/researchdata.cab.unipd.it.00001184
title: Database of observed and calculated infrared peaks for hydrogen-related defects in natural diamond

description: This database contains observed (Table A1) and calculated (Tables B1-B3) infrared peak positions (absorption frequencies, cm-1) for different hydrogen-related defects observed in diamond. If known, the hydrogen-related defect type and C-H or N-H vibrational mode is included for each peak position. The majority of data was taken from the literature and the corresponding references are included for each peak position. Some new peak data was included from FTIR spectra analyzed by the authors. Additional data regarding the computation parameters for each simulated (calculated) FTIR spectra are included in the calculated database (Tables B1-B3). A more detailed description of how the database is structured can be found in the corresponding publication at https://doi.org/10.1016/j.diamond.2024.110866.
DOI: 10.6084/m9.figshare.24749637
title: Dataset from the Paper "A Process Proposal for Requirements Engineering for Third-Party Products and a Preliminary Evaluation at Munich Re"

description: Context: When in need for a software solution, companies of all sizes prefer buying an existing commercial-off-the-shelf (COTS) product rather than investing the time and effort on developing and maintaining their own. However, purchasing the wrong COTS solution can lead to a painful and company-critical process as well. Problem: Within this context, the absence of a both repeatable as well as pragmatic approach for the selection of a suitable third-party tool remains a common problem at various companies, including Munich Re. Approach: To this end, this work combines and extends established methodologies aiming for an efficient and effective requirements engineering approach. To validate feasibility of the approach, we furthermore report on a case study at Munich Re, in which we exemplarily apply the process in-vivo for selecting a requirements modeling tool suggested to be used across all<br>development teams of the whole organization. Results: The application at Munich Re indicates the feasibility of the approach for the selection of a medium sized software solution. Impact: We encourage practitioners to extend the presented method and incorporate it into their own decision-making process for third-party tools, with the aim to making buy-decisions more objective and more efficient in the future.
DOI: 10.5281/zenodo.10589278
title: PNASNEXUS-2024-00131
DOI: 10.5281/zenodo.10589277
title: PNASNEXUS-2024-00131
DOI: 10.5281/zenodo.10589475
title: PNASNEXUS-2024-00131
DOI: 10.5281/zenodo.10303370
title: Mesure d'absorbance pour certaines espèces colorées
DOI: 10.5281/zenodo.10303371
title: Mesure d'absorbance pour certaines espèces colorées
DOI: 10.25403/upresearchdata.25053131
title: Variant and genotype information following Next-generation sequencing (NGS) and Sanger sequencing of South African patients with cystic fibrosis

description: Supplementary 1 documents the variant information for a cohort of South African patients with cystic fibrosis, patients with suspected cystic fibrosis, and suspected carriers. The variants were discovered using next generation sequencing (NGS), annotated with variant effect prediction tools, and confirmed using sanger sequencing. Some genotype information in the dataset was obtained using gene panel screening.
The figures in Supplementary 2 show the gel electrophoresis of   polymerase chain reaction (PCR) amplicons generated for sanger sequencing to confirm variants found using next generation sequencing of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in South African patients with cystic fibrosis.
Supplementary 3 shows the results of Sanger sequencing for confirmation of variants obtained using next generation sequencing for South African patients with cystic fibrosis. The quality controlled chromatograms, as well as their alignment to the CFTR gene and identification of variants are provided.
DOI: 10.25403/upresearchdata.25053131.v1
title: Variant and genotype information following Next-generation sequencing (NGS) and Sanger sequencing of South African patients with cystic fibrosis

description: Supplementary 1 documents the variant information for a cohort of South African patients with cystic fibrosis, patients with suspected cystic fibrosis, and suspected carriers. The variants were discovered using next generation sequencing (NGS), annotated with variant effect prediction tools, and confirmed using sanger sequencing. Some genotype information in the dataset was obtained using gene panel screening.
The figures in Supplementary 2 show the gel electrophoresis of   polymerase chain reaction (PCR) amplicons generated for sanger sequencing to confirm variants found using next generation sequencing of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in South African patients with cystic fibrosis.
Supplementary 3 shows the results of Sanger sequencing for confirmation of variants obtained using next generation sequencing for South African patients with cystic fibrosis. The quality controlled chromatograms, as well as their alignment to the CFTR gene and identification of variants are provided.
DOI: 10.5281/zenodo.10589570
title: Particular data point bottle blowing
DOI: 10.5281/zenodo.10589571
title: Particular data point bottle blowing
DOI: 10.5281/zenodo.10589591
title: Particular data point bottle blowing
DOI: 10.20387/bonares-kp4s-ddbj
title: Model Output Scenario Analysis 2023

description: Results of a model-based scenario analysis of 10 sites over 25 years. Approach: Reduction of nitrogen losses after winter oilseed rape before winter wheat by different residue management options. Treatments: with previous crop straw, without residues, replacement of the original crop residues with winter wheat straw. Related Publication: Rothardt & Kage, 2023: „Simulation-based Assessment of Residue Management to Mitigate N Loss Risk in Winter Wheat Production”, submitted to Nutrient Cycling in Agroecosystems.

Research domain: Plant Cultivation and Agricultural Technology

Research question: The aim of this study was to assess the site-specific impact of residue management options after the harvest of winter oilseed rape with regard to mitigate the environmental impact of intensive agriculture, i.e. N leaching losses.
DOI: 10.17031/65b0de1e9cd91
title: 1962 Marine Biological Association of the UK (MBA) European Continental Slope RV Sarsia Cruise No. P1 July
title: 1962 MBA European Continental Slope RV Sarsia Cruise No. P1 July

description: Records from the RV Sarsia Cruise No. P1 in July 1962 to the European continental slope. Samples were collected by a number of methods including long line hooks, rectangular dredge and Agassiz dredge. Records supplied by Dr. Eve Southward and collated by DASSH as part of the 'Dredging the Depths' project.
DOI: 10.25666/dataubfc-2022-10-26
title: Gaia Data Release 3
title: Troisième catalogue de Gaia

description: Gaia is a cornerstone mission in the science programme of the European Space Agency (ESA). The spacecraft construction was approved in 2006. Both the spacecraft and the payload were built by European industry. The involvement of the scientific community focusses on data processing for which the international Gaia Data Processing and Analysis Consortium (DPAC) was selected in 2007. Gaia was launched on 19 December 2013. The early third data release is based on 34 months of observations, and was issued on 13 June 2022. It contains the five-parameter astrometric solution - positions on the sky, parallaxes, and proper motions - for more than 1.4 billion sources, with a limiting magnitude of G = 21 and a bright limit of G ≈ 3. It also contains classification, astrophysical, and orbital parameters, as well as radial velocities for several tens to hundreds of millions of objects. The full description is given in https://www.cosmos.esa.int/web/gaia/dr3
description: Gaia est une mission clé du programme scientifique de l'Agence spatiale européenne (ESA), approuvée en 2006. L'implication de la communauté scientifique se concentre sur le traitement des données pour lequel le Consortium international de traitement et d'analyse des données Gaia (DPAC) a été sélectionné en 2007. Gaia a été lancé le 19 décembre 2013. Le troisième catalogue de données, publiée le 13 juin 2022, est basée sur 34 mois d'observations. Il contient la solution astrométrique à cinq paramètres - positions sur le ciel, parallaxes et mouvements propres - pour plus de 1,4 milliard de sources, avec une magnitude limite de G = 21 et une limite brillante de G ≈ 3. Il contient également des paramètres de classification, des paramètres astrophysiques, et des paramètres orbitaux, ainsi que des vitesses radiales pour plusieurs dizaines à centaines de millions d'astres. La description complète est donnée dans https://www.cosmos.esa.int/web/gaia/dr3


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description: Audience: Research
description: UpdatePeriodicity: no update
description: Project: GAIA, CNES - Centre national d'études spatiales (Another national), ESA - European Space Agency (Another international)
description: Label: SNO GAIA (SNO GAIA)
description: Observational data: Astrometric Space mission
DOI: 10.25666/dataosu-2021-09-09
title: Gaia early Data Release 3
title: Gaia early Data Release 3

description: Gaia is a cornerstone mission in the science programme of the European Space Agency (ESA). The spacecraft construction was approved in 2006. Both the spacecraft and the payload were built by European industry. The involvement of the scientific community focusses on data processing for which the international Gaia Data Processing and Analysis Consortium (DPAC) was selected in 2007. Gaia was launched on 19 December 2013. The early third data release is based on 34 months of observations, and was issued on 3 December 2020. It contains the five-parameter astrometric solution - positions on the sky (α, δ), parallaxes, and proper motions - for more than 1.4 billion sources, with a limiting magnitude of G = 21 and a bright limit of G ≈ 3. The full description is given in https://www.cosmos.esa.int/web/gaia/earlydr3
description: Audience: Research
description: UpdatePeriodicity: no update
description: Project: GAIA, CNES - Centre national d'études spatiales (Another national), ESA - European Space Agency (Another international)
description: Label: SNO GAIA (SNO GAIA)
description: Observational data: Astrometric Space mission
DOI: 10.25666/dataosu-2018-06-25
title: Gaia Data Release 2
title: 2ème catalogue de données Gaia

description: Gaia is a cornerstone mission in the science programme of the European Space Agency (ESA). The spacecraft construction was approved in 2006,. Both the spacecraft and the payload were built by European industry. The involvement of the scientific community focusses on data processing for which the international Gaia Data Processing and Analysis Consortium (DPAC) was selected in 2007. Gaia was launched on 19 December 2013. The second data release is based on 22 months of observations, and was issued on 25 April 2018. It contains the five-parameter astrometric solution - positions on the sky (α, δ), parallaxes, and proper motions - for more than 1.3 billion sources, with a limiting magnitude of G = 21 and a bright limit of G ≈ 3. The full description is given in https://www.cosmos.esa.int/web/gaia/dr2
description: Audience: Research
description: UpdatePeriodicity: no update
description: Project: GAIA, CNES - Centre national d'études spatiales (Another national), ESA - European Space Agency (Another international)
description: Observational data: Astrometric Space mission
DOI: 10.25666/dataosu-2017-03-10-02
title: Gaia Data Release 1
title: 1er catalogue de données Gaia

description: Gaia is a cornerstone mission in the science programme of the European Space Agency (ESA). The spacecraft construction was approved in 2006, following a study in which the original interferometric concept was changed to a direct-imaging approach. Both the spacecraft and the payload were built by European industry. The involvement of the scientific community focusses on data processing for which the international Gaia Data Processing and Analysis Consortium (DPAC) was selected in 2007. Gaia was launched on 19 December 2013 and arrived at its operating point, the second Lagrange point of the Sun-Earth-Moon system, a few weeks later. The commissioning of the spacecraft and payload was completed on 19 July 2014. The first data release was issued on 16 September 2016.
description: Audience: Research
description: UpdatePeriodicity: no update
description: Project: GAIA, CNES - Centre national d'études spatiales (Another national), ESA - European Space Agency (Another international)
description: Observational data: Astrometric Space mission
DOI: 10.5281/zenodo.10589514
title: Raw data from "Morphology-resolved search for mutations in non- responders to anti-EGFR therapy in colorectal carcinoma" part 1/7

description: We performed targeted sequencing of morphological regions of primary colon cancer tumors for patients receiveing anti-EGFR (KRAS wild type) treatment as first line in metastatic setting. These are the raw FASTQ files, part 1 out of 7.
DOI: 10.5281/zenodo.10589515
title: Raw data from "Morphology-resolved search for mutations in non- responders to anti-EGFR therapy in colorectal carcinoma" part 1/7

description: We performed targeted sequencing of morphological regions of primary colon cancer tumors for patients receiveing anti-EGFR (KRAS wild type) treatment as first line in metastatic setting. These are the raw FASTQ files, part 1 out of 7.
DOI: 10.34740/kaggle/ds/4122223
title: Gesture Calculator Image Dataset
DOI: 10.17031/65b1057de2397
title: 1968 Marine Biological Association of the UK (MBA) Bay of Biscay RV Sarsia Cruise July
title: 1968 MBA Bay of Biscay RV Sarsia Cruise July

description: Records from the RV Sarsia Cruise in July 1968 around the underwater canyons in the Bay of Biscay, off the north coast of Spain. Samples were collected using either a scallop dredge or an anchor dredge. Records supplied by Dr. Eve Southward and collated by DASSH as part of the 'Dredging the Depths' project.
DOI: 10.25666/dataubfc-2022-05-20
title: Antimicrobial Activity and DFT Studies of a Novel Set of Spiropyrrolidines Tethered with Thiochroman-4-one/Chroman-4-one Scaffolds

description: A novel series of 14 spiropyrrolidines bearing thiochroman-4-one/chroman-4-one, and oxindole/acenaphthylene-1,2-dione moieties were synthesized and characterized by spectroscopic techniques, as well as by three X-ray diffraction studies, corroborating the stereochemistry. Quantum chemical calculations studies, using the DFT approach, were performed to rationalize the stereochemical outcome. These N-heterocycles were evaluated for their antibacterial and antifungal activities against some pathogenic organisms. Several compounds displayed moderate to excellent activity towards the screened microbe strains in the study compared to Amoxicillin (AMX), Ampicillin (AMP), and Amphotericin B. Furthermore, a structural activity relationship (SAR) was established considering the synthesized compounds. Pharmacokinetic studies reveal that these derivatives exhibit an acceptable predictive ADMET profile (Absorption, Distribution, Metabolism, Excretion and Toxicity) and good drug-likeness.
description: Audience: University: master, Research
description: UpdatePeriodicity: no update
description: AdditionnalInformation: This work was supported by the LR11ES39 Grant from the Ministry of Higher Education and Scientific Research, Tunisia.
description: AdditionnalInformation: Ce travail a été soutenu par la subvention LR11ES39 du Ministère de l'Enseignement Supérieur et de la Recherche Scientifique, Tunisie.
description: Project: Elaboration des composés héterocycliques antidiabétiques et antimicrobiens, Calcul Quebec (Another international), Compute Canada (Another international)
description: Experimental data: Organic synthesis
Crystal structure
Modelling
Biological activity
DFT computing
description: Données expérimentales: Synthèse organique
Structure cristalline
Modélisation
Activité biologique
Calcul DFT
description: Simulation or computational data: Docking simulations
description: Données computationnelles ou de simulation: Simulations de docking
DOI: 10.5281/zenodo.10498019
title: Cut&Run H3K27ac inVivo HFD 30mg

description: For CUT&RUN against H3K27ac in mouse liversamples, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-9521048) according to the manufacturer’s instructions with minor modifications.For in vivo experiments, approximately 200 mg of liver tissue was homogenized in DMEM(Gibco, 41966-029) using a potter tube and a glass pestle with 12 strokes. The resulting cellsuspension was then centrifuged at 1000 xg for 5 minutes at 4°C. The samples weresubsequently washed with 1 ml PBS (Sigma D8537) supplemented with cOmplete proteaseinhibitor cocktail (Roche, CO-RO SKU 11697498001) and 10 mM sodium butyrate (SigmaAldrich, 303410), followed by centrifugation under the same conditions. The following stepswere performed in presence of protease inhibitors and sodium butyrate. Washed cells wereresuspended in 1 ml of NIB with 0.3% IGEPAL CA-630 (Sigma, I8896) and incubated on icefor 5 minutes. The nuclear suspension was then centrifuged at 1000 xg for 5 minutes at 4°C.The resulting nuclear pellet was washed with 1 ml of nucleus isolation buffer (NIB) (15 mMTris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mMCaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (CarlRoth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth,Page 37Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) without IGEPAL CA-630,and the nuclei were subsequently filtered through a 30 μm strainer. An additional washing stepwas performed with 1 ml of NIB, and after centrifugation, and the nuclei resuspended in 100μl of NIB without IGEPAL CA-630. The nuclei were counted, and 0.5 x 106 nuclei were usedfor the subsequent procedure, either in step 7 for ACLY ChIC (Version 1.0 EpiCypher, SKU:14-1048) or in step 14 for H3K9ac and H3K27ac ChIC (Version 3.3 EpiCypher, SKU: 14-1048)of the manufacturer's protocol.For each reaction, 2 μg of antibody was used (Diagenode C15410196). Sequencinglibraries were prepared using NEBNext Ultra II library preparation procedure (New EngladBiolabs, E7645L) with modifications according to NGS Library preparation of CUTANAChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048, Version 1.0), and then assessed for qualityand quantity by BioAnalyzer (Agilent). Generated libraries were sequenced on an IlluminaNovaSeq instrument (150 bp, paired-end)
DOI: 10.5281/zenodo.10498020
title: Cut&Run H3K27ac inVivo HFD 30mg

description: For CUT&RUN against H3K27ac in mouse liversamples, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-9521048) according to the manufacturer’s instructions with minor modifications.For in vivo experiments, approximately 200 mg of liver tissue was homogenized in DMEM(Gibco, 41966-029) using a potter tube and a glass pestle with 12 strokes. The resulting cellsuspension was then centrifuged at 1000 xg for 5 minutes at 4°C. The samples weresubsequently washed with 1 ml PBS (Sigma D8537) supplemented with cOmplete proteaseinhibitor cocktail (Roche, CO-RO SKU 11697498001) and 10 mM sodium butyrate (SigmaAldrich, 303410), followed by centrifugation under the same conditions. The following stepswere performed in presence of protease inhibitors and sodium butyrate. Washed cells wereresuspended in 1 ml of NIB with 0.3% IGEPAL CA-630 (Sigma, I8896) and incubated on icefor 5 minutes. The nuclear suspension was then centrifuged at 1000 xg for 5 minutes at 4°C.The resulting nuclear pellet was washed with 1 ml of nucleus isolation buffer (NIB) (15 mMTris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mMCaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (CarlRoth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth,Page 37Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) without IGEPAL CA-630,and the nuclei were subsequently filtered through a 30 μm strainer. An additional washing stepwas performed with 1 ml of NIB, and after centrifugation, and the nuclei resuspended in 100μl of NIB without IGEPAL CA-630. The nuclei were counted, and 0.5 x 106 nuclei were usedfor the subsequent procedure, either in step 7 for ACLY ChIC (Version 1.0 EpiCypher, SKU:14-1048) or in step 14 for H3K9ac and H3K27ac ChIC (Version 3.3 EpiCypher, SKU: 14-1048)of the manufacturer's protocol.For each reaction, 2 μg of antibody was used (Diagenode C15410196). Sequencinglibraries were prepared using NEBNext Ultra II library preparation procedure (New EngladBiolabs, E7645L) with modifications according to NGS Library preparation of CUTANAChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048, Version 1.0), and then assessed for qualityand quantity by BioAnalyzer (Agilent). Generated libraries were sequenced on an IlluminaNovaSeq instrument (150 bp, paired-end)
DOI: 10.17031/65afcd59e7236
title: 1966 Marine Biological Association of the UK (MBA) Bay of Biscay RV Sarsia Cruise July
title: 1966 MBA Bay of Biscay RV Sarsia Cruise July

description: Records from the RV Sarsia Cruise in July 1966 along the European continental slope along the north coast of Spain. Samples were collected using a number of methods including Holme grab, Anchor dredge and Agassiz trawl. Records supplied by Dr. Eve Southward and collated by DASSH as part of the 'Dredging the Depths' project.