cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0pr7tps
title: CIL:13993, Homo sapiens. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
description: This image is part of Fig 2B from the reference article PMID 20212317. HeLa cells were treated with anti-SENP6 siRNA and double thymidine block for 48 h. Cells were fixed and stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab), Bub1 (green, Alexa Fluor 488 conjugated secondary Ab) and Hoechest 33342 (blue). 

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).
DOI: 10.6075/j07s7mgx
title: CIL:13991, Homo sapiens. In Cell Image Library

description: This image is part of Fig. 2B from the reference article PMID 20212317. HeLa cells were treated with control siRNA and double thymidine block for 48 h. Cells were fixed and stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab), Bub1 (green, Alexa Fluor 488 conjugated secondary Ab) and Hoechest 33342 (blue). 

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
DOI: 10.6075/j0g15zjs
title: CIL:14005, Homo sapiens. In Cell Image Library

description: Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins Hec1 (green, Alexa Fluor 488 conjugated secondary Ab). Cells were simultaneously stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab) and Hoechst 33342 (blue).

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).

Image Reference: PMID 20212317
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
DOI: 10.6075/j05b016j
title: CIL:12172, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP OFF cells expressing mutant TrAP-INCENP F802A. See Fig 6C panel 9 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j02v2dw2
title: CIL:12274, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP WT. Endogenous Aurora B is green and DAPI (DNA) is blue. See Supplementary Fig 1E panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0cc0zc5
title: CIL:12211, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase in INCENP-OFF cells expressing TrAP-INCENP F802A. Histone H3 modification H3-S10 ph shown in green and DAPI (DNA) in blue. See Supplementary Fig 3G panels 12-14 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0xk8d7t
title: CIL:12156, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B(green) in INCENPOFF cells expressing TrAP-INCENPWT. See Fig 4C panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j06w98v6
title: CIL:14008, Homo sapiens. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
description: Hela cells were treated with anti-SENP6 siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-E (green, secondary Ab conjugated with Alexa Fluor 488) Cells were simultaneously stained for centromeres (CREST, red, secondary Ab conjugated with Alexa Fluor 568) and Hoechst 33342 (blue).

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).

Image Reference: PMID 20212317
DOI: 10.6075/j0ks6q86
title: CIL:14004, Homo sapiens. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
description: Hela cells were treated with anti-SENP6 siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-O (green, Alexa Fluor 488 conjugated secondary Ab) Cells were simultaneously stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab) and Hoechst 33342 (blue).

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).

Image Reference: PMID 20212317
DOI: 10.6075/j02v2dvm
title: CIL:14003, Homo sapiens. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
description: Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-O (green, Alexa Fluor 488 conjugated secondary Ab) Cells were simultaneously stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab) and Hoechst 33342 (blue).

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).

Image Reference: PMID 20212317
DOI: 10.6075/j0gt5kx3
title: CIL:12201, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2D panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0tt4pqc
title: CIL:14002, Homo sapiens. In Cell Image Library

description: This image is part of Fig 3A from the reference article PMID 20212317. Hela cells were treated with anti-SENP6 siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red, Alexa Flour 568), CenpH antibody (green, Alexa Fluor 488) and Hoechst 33342 (blue). 

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
DOI: 10.6075/j0g44p1d
title: CIL:12196, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A . Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2C panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0z89b3s
title: CIL:12251, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Cytokinesis localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP WT which co-localizes with endogenous survivin (green). DAPI (DNA) is blue. See Supplementary Fig 1D panels 13-16 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j08051bq
title: CIL:14007, Homo sapiens. In Cell Image Library

description: Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-E (green, Alexa Fluor 488 conjugated secondary Ab) Cells were simultaneously stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab) and Hoechst 33342 (blue). 

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).

Image Reference: PMID 20212317
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
DOI: 10.6075/j0xg9pw0
title: CIL:12213, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase in INCENP-OFF cells expressing TrAP-INCENP F802A. Histone H3 modification H3-T3ph shown in red and H3-S28ph in green. DAPI (DNA) is blue. See Supplementary Fig 3G panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j03t9fxj
title: CIL:12210, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase in INCENP-OFF cells expressing TrAP-INCENP F802A. Histone H3 modification H3-S10ph shown in green and DAPI (DNA) in blue. See Supplementary Fig 3G panels 5-7 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0k9369g
title: CIL:13994, Homo sapiens. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
description: This image is part of Fig 2B from the reference article PMID 20212317. HeLa cells were treated with anti-SENP6 siRNA and double thymidine block for 48 h. Cells were fixed and stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab), Bub1 (green, Alexa Fluor 488 conjugated secondary Ab) and Hoechest 33342 (blue). 

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).
DOI: 10.6075/j0k35sc3
title: CIL:14001, Homo sapiens. In Cell Image Library

description: This image is part of Fig 3A from the reference article PMID 20212317. Hela cells were treated with control siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red, Alexa Flour 568), CenpH antibody (green, Alexa Fluor 488) and Hoechst 33342 (blue).

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568