DOI: 10.5061/dryad.xpnvx0krq
title: Time-integrated δ²H in n-alkanes and carbohydrates from boreal needles reveal intra-annual physiological and environmental signals

description: This dataset is supplementary to the article, 'Time-integrated δ²H in
 n-alkanes and carbohydrates from boreal needles reveal intra-annual
 physiological and environmental signals'. In the article, we used
 time integrated (a.k.a., time aggregated) variables, to identify
 meaningful physiological and environmental interpretations from the
 hydrogen isotope ratio (δ²H) of leaf n-alkanes and carbohydrates. We
 conducted an intra-annual survey of five Scots Pine (Pinus sylvestris L.)
 trees in a coniferous forest in Hyytiälä, Finland. During 2019, we sampled
 one year-old needles and current-year needles from the trees, biweekly. We
 measured the δ2H of their n-alkanes (δ2Halkane), water-soluble
 carbohydrates (δ²HWSC) and starch (δ²Hstarch). We also measured the
 concentrations of total lipid extracts, water-soluble carbohydrates and
 starch. We modelled source water δ2H, leaf water δ2H, and leaf water 2H
 enrichment above source water; model specifications are described
 thoroughly in the original article. This dataset includes time-integrated
 versions of these modelled values used for analysis in this article.
description: # Time-integrated δ²H in *n*-alkanes and carbohydrates from boreal needles
 reveal intra-annual physiological and environmental signals
 [https://doi.org/10.5061/dryad.xpnvx0krq](https://doi.org/10.5061/dryad.xpnvx0krq) ## Description of the data and file structure This dataset is supplementary to the article, 'Time-integrated δ²H in *n*-alkanes and carbohydrates from boreal needles reveal intra-annual physiological and environmental signals'. In the article, we used time integrated (a.k.a., time aggregated) variables, to identify meaningful physiological and environmental interpretations from the hydrogen isotope ratio (δ²H) of leaf *n*-alkanes and carbohydrates. We conducted an intra-annual survey of five Scots Pine (*Pinus sylvestris* L.) trees in a coniferous forest in Hyytiälä, Finland. During 2019, we sampled one year-old needles and current-year needles from the trees, biweekly. We measured the δ²H of their *n*-alkanes (δ²Halkane), water-soluble carbohydrates (δ²HWSC) and starch (δ²Hstarch). We also measured the concentrations of total lipid extracts, water-soluble carbohydrates and starch. We modelled source water δ²H, leaf water δ²H, and leaf water ²H enrichment above source water; model specifications are described thoroughly in the original article. This dataset includes time-integrated versions of these modelled values used for analysis in this article. ### Files and variables #### File: Angove\_etal\_NANSC\_2025.xlsx **Description:** Three Microsoft Excel worksheets, each containing a different dataset. Missing values are marked as 'NA'. ##### Variables Carbohydrate_d2H worksheet * Tree_ID: The identification number for an individual tree * d2H: Measured hydrogen isotope ratio (VSMOW, ‰) * Date: Date of sampling (Format: YYYY-MM-DD) * Sample_type: '0N' or '1N'. '0N' = Current-year needles, '1N' = One year-old needles * Pool: The compound or mixture being measured * Modelled_water_D2H.Day0: The modelled needle water hydrogen isotope enrichment above source water (‰) on the day of sampling  * CG_peclet_H_abs.Day0: The modelled needle water hydrogen isotope ratio (VSMOW, ‰) on the day of sampling * Gapfilledmeasured_d2Hsw.Day0: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰) on the day of sampling * Modelled_water_D2H.Day_minus1: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the day of sampling and the day that preceded it  * CG_peclet_H_abs.Day_minus1: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the day that preceded it * Gapfilledmeasured_d2Hsw.Day_minus1: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the day that preceded it * Modelled_water_D2H.Day_minus2: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the day of sampling and the two days preceding it  * CG_peclet_H_abs.Day_minus2: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the two days preceding it * Gapfilledmeasured_d2Hsw.Day_minus2: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the two days preceding it * Modelled_water_D2H.Day_minus3: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the day of sampling and the three days preceding it  * CG_peclet_H_abs.Day_minus3: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the three days preceding it * Gapfilledmeasured_d2Hsw.Day_minus3: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the three days preceding it * Modelled_water_D2H.Day_minus4: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the day of sampling and the four days preceding it  * CG_peclet_H_abs.Day_minus4: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the four days preceding it * Gapfilledmeasured_d2Hsw.Day_minus4: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the day of sampling and the four days preceding it Alkane_d2H worksheet * Date: Date of sampling (Format: YYYY-MM-DD) * Sample_type: '0N' or '1N'. '0N' = Current-year needles, '1N' = One year-old needles * d2H: Measured hydrogen isotope ratio (VSMOW, ‰) * Tree_ID: The identification number for an individual tree * Pool: The compound or mixture being measured * Modelled_water_D2H.Week_minus0: The modelled needle water hydrogen isotope enrichment above source water (‰) on the day of sampling * CG_peclet_H_abs.Week_minus0: The modelled needle water hydrogen isotope ratio (VSMOW, ‰) on the day of sampling * Gapfilledmeasured_d2Hsw.Week_minus0: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰) on the day of sampling * Modelled_water_D2H.Week_minus1: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the week preceding sampling * CG_peclet_H_abs.Week_minus1: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the week preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus1: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the week preceding sampling * Modelled_water_D2H.Week_minus2: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the two weeks preceding sampling  * CG_peclet_H_abs.Week_minus2: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the two weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus2: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the two weeks preceding sampling * Modelled_water_D2H.Week_minus3: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the three weeks preceding sampling * CG_peclet_H_abs.Week_minus3: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the three weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus3: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the three weeks preceding sampling * Modelled_water_D2H.Week_minus4: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the four weeks preceding sampling  * CG_peclet_H_abs.Week_minus4: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the four weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus4: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the four weeks preceding sampling * Modelled_water_D2H.Week_minus5: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the five weeks preceding sampling  * CG_peclet_H_abs.Week_minus5: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the five weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus5: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the five weeks preceding sampling * Modelled_water_D2H.Week_minus6: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the six weeks preceding sampling  * CG_peclet_H_abs.Week_minus6: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the six weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus6: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the six weeks preceding sampling * Modelled_water_D2H.Week_minus7: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the seven weeks preceding sampling  * CG_peclet_H_abs.Week_minus7: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the seven weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus7: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the seven weeks preceding sampling * Modelled_water_D2H.Week_minus8: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the eight weeks preceding sampling  * CG_peclet_H_abs.Week_minus8: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the eight weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus8: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the eight weeks preceding sampling * Modelled_water_D2H.Week_minus9: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the nine weeks preceding sampling  * CG_peclet_H_abs.Week_minus9: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the nine weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus9: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the nine weeks preceding sampling * Modelled_water_D2H.Week_minus10: The modelled needle water hydrogen isotope enrichment above source water (‰), an aggregated average of the ten weeks preceding sampling  * CG_peclet_H_abs.Week_minus10: The modelled needle water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the ten weeks preceding sampling * Gapfilledmeasured_d2Hsw.Week_minus10: Gapfilled measured source water hydrogen isotope ratio (VSMOW, ‰), an aggregated average of the ten weeks preceding sampling Concentrations worksheet * Date: Date of sampling (Format: YYYY-MM-DD) * Sample_type: '0N' or '1N'. '0N' = Current-year needles, '1N' = One year-old needles * Conc_mgg_mean: Mean concentration (mg per g dried sample) * Conc_mgg_se: Standard error of concentration (mg per g dried sample) * Pool: The compound or mixture being measured