cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/8cvbh-cth27
title: Caulobacter crescentus ftsE del

description: Caulobacter crescentus, ftsE, FtsZ, Long cell, PYE, Xylose induction
description: <strong>Tilt Series Date:</strong> 2016-09-04</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Goley ED at Johns Hopkins University School of Medicine</p>
<strong>Purification / Growth Conditions / Treatment:</strong> When OD600 reaches 0.30, 2 ml medium was spun down gently (3000 rpm for 3 min) and the resuspension was 100 ul for cryo-grid preparation.</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 190/A2. defocus: -12um. magnification: 22500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-09-04-8/rawdata/</p>square10011.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-09-04-8/rawdata/square10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        square10011_part121_5.rec,  Reconstruction (Pixel Size 1.92 nm), 311.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-09-04-8/3dimage_78666/square10011_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0fye4-a3t69
title: Myxococcus xanthus SA6888

description: Strain SA6888 (minor pilin cluster 1+2 deletion, Δ mxan1021-1017;  Δmxan0364-0359).Grid box YWC224
description: <strong>Tilt Series Date:</strong> 2016-09-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-15-50/rawdata/</p>20160915_YWC224_10050.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-15-50/rawdata/20160915_YWC224_10050.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160915_YWC224_10050_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-15-50/3dimage_79197/20160915_YWC224_10050_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6rsan-mzn20
title: Pombe Cryosections

description: These cells were high pressure frozen after the majority of the rings were around mid-division.
description: <strong>Tilt Series Date:</strong> 2015-05-30</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Manual/Raptor</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-30-31/rawdata/</p>053015_cryosection1_square6_tomo0003.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-30-31/rawdata/053015_cryosection1_square6_tomo0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        053015_cryosection1_square6_tomo0003.rec,  Reconstruction (Pixel Size 2.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-30-31/3dimage_57698/053015_cryosection1_square6_tomo0003.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0bwmz-06n46
title: Planktonic cell of Proteus

description: Planktonic cell, PG layer, Proteus mirabillis
description: <strong>Tilt Series Date:</strong> 2016-06-18</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Purification / Growth Conditions / Treatment:</strong> OD=6, 4 ul loading. blotting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 195/A2. defocus: -10um. magnification: 22500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-18-10/rawdata/</p>square20013.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-18-10/rawdata/square20013.mrc"
        > <i class="download icon"></i> Download </a></p>

        square20013.rec,  Reconstruction (Pixel Size 1.92 nm), 622.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-18-10/3dimage_75855/square20013.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eq2p5-yaf64
title: Hippocampal Neurons (DIV 7)

description: Plunge frozen at DIV 7
description: <strong>Tilt Series Date:</strong> 2015-05-03</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 7. Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-03-13/rawdata/</p>050315_hipp_neuron_7div_grid3_hole4_tomo_20010.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-03-13/rawdata/050315_hipp_neuron_7div_grid3_hole4_tomo_20010.mrc"
        > <i class="download icon"></i> Download </a></p>

        050315_hipp_neuron_7div_grid3_hole4_tomo_20010_full.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-03-13/3dimage_55751/050315_hipp_neuron_7div_grid3_hole4_tomo_20010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.tzmm5j
title: Occurrence Download

description: A dataset containing 43 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cyclachaena xanthiifolia",
 "Country is Estonia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 43 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048002-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/cq2s4-1rx98
title: T5_1550Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1550 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-03-07</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. The humidifier was not working. I wouldn't really trust the conditions. See pages 104-105</p>
<strong>Sample Preparation:</strong> The sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-07-1/rawdata/</p>sq130001.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-07-1/rawdata/sq130001.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq130001.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-07-1/3dimage_82975/sq130001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/551k8-7mr36
title: immuno_excyto

description: This is an immuno labeled sample. The extracellular cytochrome supposed to be the nanowire is immuno labeled i.e. the primary antibody was against the extracellular cytochrome, and a gold conjugated secondary against the primary was used. This sample is fixed.
description: <strong>Tilt Series Date:</strong> 2015-06-07</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> On imaging it is seen that there are way too many gold beads and also there is ice on the sample.</p>
<strong>Sample Preparation:</strong> This is wt chemostat sample. Cu finder grids were used (the usual one). Blotting conditions- Blot force -6, blot time -3 , drain time - 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-07-1/rawdata/</p>immunoexcyto_manual1.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-07-1/rawdata/immunoexcyto_manual1.mrc"
        > <i class="download icon"></i> Download </a></p>

        immunoexcyto_manual1_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-07-1/3dimage_58505/immunoexcyto_manual1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a8h06-pwq46
title: Proteus

description: Swarmer cell, long cell, Proteus mirabillis .  [added 2018-08-03 20:59:25] Proteus cell
description: <strong>Tilt Series Date:</strong> 2015-11-19</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33599x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university. Preparethe cell and design the research.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> This sample is prepared with the help of George Aucer, Ph. D student at Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Sample Preparation:</strong> OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-11-19-12/3dimage_71083/</p>atlas3_at20002_part121_54.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-11-19-12/3dimage_71083/atlas3_at20002_part121_54.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas3_at20002.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-11-19-12/rawdata/atlas3_at20002.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7ty82-27e62
title: Myxococcus xanthus SA6892

description: Strain SA6892 (minor pilin cluster 2+3 deletion, Δmxan1021-1017; Δmxan1369-1365).Grid box YWC222
description: <strong>Tilt Series Date:</strong> 2016-08-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-75/rawdata/</p>20160822_YWC222_10076.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-75/rawdata/20160822_YWC222_10076.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160822_YWC222_10076_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-75/3dimage_78404/20160822_YWC222_10076_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v6555-aa256
title: Planktonic cell of Proteus

description: Planktonic cell, PG layer, Proteus mirabillis
description: <strong>Tilt Series Date:</strong> 2016-06-22</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Purification / Growth Conditions / Treatment:</strong> OD=6, 4 ul loading. blotting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 195/A2. defocus: -10um. magnification: 22500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-22-29/rawdata/</p>square20008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-22-29/rawdata/square20008.mrc"
        > <i class="download icon"></i> Download </a></p>

        square20008_full.rec,  Reconstruction (Pixel Size 1.92 nm), 311.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-22-29/3dimage_75967/square20008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/91eza-k0a67
title: T5_1550Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1550 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-03-10</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. The humidifier was not working. I wouldn't really trust the conditions. See pages 104-105</p>
<strong>Sample Preparation:</strong> The sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-2/rawdata/</p>sq7_T5_1693cysAulabelled0002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-2/rawdata/sq7_T5_1693cysAulabelled0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq7_T5_1693cysAulabelled0002.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-2/3dimage_83102/sq7_T5_1693cysAulabelled0002.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qyj43-r4021
title: V.cholerae

description: This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.
description: <strong>Tilt Series Date:</strong> 2017-01-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Yi-wei</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: -60, 60.         dosage: 160e/A2. defocus: -6um. magnification: 27500.</p>
  psa2017-01-06-17_slicer17794.jpg: DNA?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-17/rawdata/</p>atlas30017.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
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        atlas30017_part121_4.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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