cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2017.501
title: สภาพและปัญหาของครูในการสร้างรอยเชื่อมต่อทางการศึกษาของเด็กอนุบาลเพื่อเข้าสู่ชั้นประถมศึกษา

description: การวิจัยนี้มุ่งศึกษาสภาพและปัญหาของครูในการสร้างรอยเชื่อมต่อทางการศึกษาของเด็กอนุบาลเพื่อเข้าสู่ชั้นประถมศึกษาใน 3 ด้าน ได้แก่ การวางแผนการดำเนินงานการสร้างรอยเชื่อมต่อ การสร้างความร่วมมือกับผู้ที่เกี่ยวข้องในการสร้างรอยเชื่อมต่อ และการส่งเสริมความพร้อมทางการเรียน ตัวอย่างคือ ครูประจำชั้นของเด็กอายุ 5 ถึง 6 ปี ภาคเรียนที่ 2 ปีการศึกษา 2560 จำนวน 323 คน เขตกรุงเทพมหานคร เครื่องมือที่ใช้ในการวิจัยคือ แบบสอบถาม แบบสัมภาษณ์ การวิเคราะห์ข้อมูลโดยการแจกแจงความถี่ หาค่าร้อยละ ค่าเฉลี่ย ส่วนเบี่ยงเบนมาตรฐาน และการวิเคราะห์เนื้อหาผลการวิจัยพบว่า1. สภาพของครูในการสร้างรอยเชื่อมต่อทางการศึกษาของเด็กอนุบาลเพื่อเข้าสู่ชั้นประถมศึกษาอยู่ในระดับมาก (x̅=3.99) ด้านที่มีค่าเฉลี่ยคะแนนสูงสุดคือ การส่งเสริมความพร้อมทางการเรียน (x̅=4.26) รองลงมาคือ การวางแผนการดำเนินงานการสร้างรอยเชื่อมต่อ (x̅=3.91) และค่าเฉลี่ยคะแนนต่ำสุดคือ การสร้างความร่วมมือกับผู้ที่เกี่ยวข้องในการสร้างรอยเชื่อมต่อ (x̅=3.81) ตามลำดับ2. ปัญหาของครูในการสร้างรอยเชื่อมต่อทางการศึกษาของเด็กอนุบาลเพื่อเข้าสู่ชั้นประถมศึกษาใน 3 ด้าน ได้แก่ ด้านการวางแผนการดำเนินงานการสร้างรอยเชื่อมต่อพบว่า (1) การไม่เชื่อมต่อระหว่างหลักสูตรแกนกลางการศึกษาขั้นพื้นฐานและหลักสูตรการศึกษาปฐมวัย (2) การมีนโยบายที่เร่งให้เด็กอ่านออกเขียนได้ในชั้นประถมศึกษาปีที่ 1 และการวัดประเมินผล ด้านการสร้างความร่วมมือกับผู้ที่เกี่ยวข้องในการสร้างรอยเชื่อมต่อพบว่า (1) การขาดความเข้าใจในการเรียนการสอนระดับอนุบาล พัฒนาการและการเรียนรู้ของผู้บริหาร ครูประถมศึกษา และผู้ปกครอง (2) การขาดโอกาสในการสร้างความรู้ความเข้าใจร่วมกันของครูอนุบาลและครูประถมศึกษา และด้านส่งเสริมความพร้อมทางการเรียนพบว่า (1) ความแตกต่างของเด็กและครอบครัวเป็นรายบุคคลที่ส่งผลต่อความพร้อมของเด็ก (2) ผู้ปกครองมีความคาดหวัง และความกังวลใจในการสอบเข้าเรียนชั้นประถมศึกษาปีที่ 1 (3) ครูประถมศึกษาปีที่ 1 มีความคาดหวังเกี่ยวกับความพร้อมด้านวิชาการที่เกินกว่าพัฒนาการของเด็ก
DOI: 10.5281/zenodo.4462823
title: Pressure and Inertial sensing drifter data for glacial hydrology flow path.

description: Raw data for paper titled 'Topology and pressure distribution reconstruction of an englacial channel' The dataset consists of field measurements conducted on Austre Brøggerbreen, Ny-Ålesund, Svalbard. The dataset consists of: (1)Raw englacial data (6 deployments), 2019; (2) Raw supraglacial data (11 deployments), 2019; (3) Average GNSS drifter path along the supraglacial channel, 2019; (4) GNSS drifter path along the englacial channel, 2020; (5) Englacial river mapped from a satellite image. The experimental work was conducted between 30.06.2019 and 05.07.2019, during the period of the main spring snow melt. All drifters were recovered by hand from the river.
description: The fieldwork for this project was funded by the H2020 INTERACT Transnational Access project DeepSense 2019, University of Oslo Industrial Liason, University of Oslo Garpen Stipend, the Department of Geosciences University of Oslo, the Research Council of Norway funded MAMMAMIA project (grant number 301837 MAMMAMIA: Multi-scale multi-method analysis of mechanisms causing ice acceleration) and the Svalbard Environmental protection fund (17/31). This research was further funded by the European Research Council (grant no. ICEMASS) and the Research Council of Norway (grant no. 223254 – NTNU AMOS). The 2020 englacial channel data is from the fieldwork conducted in summer 2020, which was funded by the Research Council of Norway via an Arctic Field Grant to AA (Dynamics of glacial channels, RiS ID 11423). This study is a contribution to the Svalbard Integrated Arctic Earth Observating System (SIOS). We acknowledge support from the team of the NPI Sverdrup station, particularly Vera Sklet, during the fieldwork. Asko Ristolainen and Astrid Tesaker helped during the 2020 field campaign. The drifters used in this study were originally designed by Jeffrey A. Tuhtan and Juan Francisco Fuentes-Perez.
DOI: 10.6084/m9.figshare.13641281
title: Supplemental Table S2

description: <b>Table S2 AX supplementation human study literature review</b>
DOI: 10.6084/m9.figshare.13641281.v1
title: Supplemental Table S2

description: <b>Table S2 AX supplementation human study literature review</b>
DOI: 10.6084/m9.figshare.13641278
title: Supplemental Table S1

description: Table S1 Total fat oxidation before and after training (Date present as mean±S.E.)
DOI: 10.6084/m9.figshare.13641278.v1
title: Supplemental Table S1

description: Table S1 Total fat oxidation before and after training (Date present as mean±S.E.)
DOI: 10.25345/c57n54
title: MassIVE MSV000086764 - Phosphoproteomics of ATR Signaling in Prophase I of Mouse Meiosis
title: Here we used phosphoproteomics to define meiosis-specific ATR signaling events during prophase I in mice following chemical and genetic ablation of ATR signaling. Quantitative analysis of phosphoproteomes obtained after tissue-specific genetic ablation of the ATR activating 9-1-1 complex or treatment with ATR inhibitor revealed over 32,000 phosphorylation sites from testes samples, of which 892 phosphorylation sites were found to be dependent on both 911 and ATR.
DOI: 10.6084/m9.figshare.13641353.v1
title: Supplemental File 1

description: Gene annotations for differentially expressed genes <br>
DOI: 10.6084/m9.figshare.13641353
title: Supplemental File 1

description: Gene annotations for differentially expressed genes <br>
DOI: 10.6084/m9.figshare.13641359
title: Supplemental File 2

description: File with gene annotations for genes with SNPs identified in them for different experimental conditions. <br>
DOI: 10.6084/m9.figshare.13641359.v1
title: Supplemental File 2

description: File with gene annotations for genes with SNPs identified in them for different experimental conditions. <br>
DOI: 10.14457/cu.the.2018.76
title: Steady-state determination of continuous powder mixing by near infrared spectroscopy as process analytical technology

description: Powder mixing is one of an important unit operation in pharmaceutical manufacturing of solid dosage forms. Continuous mixing was developed as an alternative method to reduce disadvantages of traditional batch mixing processes. This process was originally developed using horizontal cylindrical mixer equipped with Process Analytical Technology (PAT) tools for real-time monitoring. In the present study, a vertical cylindrical mixer was developed and evaluated. Near Infrared (NIR) spectra obtained by monitoring the mixing process were pretreated with Multiplicative Scatter Correction (MSC), second derivative treatment and Norris-William smoothing in the 6800 - 8700 cm-1 range before a calibration model using PLS was developed using X-ray powder diffractometry (XRPD) as a primary method. Concentrations of mixtures in steady-state and mixing phases were identified after changing impeller alignments (aligned and opposite) and distances between impellers (0.5 and 1.0 inch). Blend quality was found to be affected by both the impeller alignment and impeller distance. Steady-state phases, onset time, mixing performance, measurement robustness and accuracy of final mixture concentrations were evaluated. It was found that aligned impeller with 0.5-inch distance shows the best mixing set-up leading to higher mixture homogeneity. However, onset time to reach steady-state was compromised.
DOI: 10.14457/cu.the.2018.77
title: Effects of 4,5,4'-trihydroxy-3,3'-dimethoxybibenzyl from Dendrobium ellipsophyllum on induction of apoptosis and inhibition of cancer stem-like cells in human lung cancer H460 cells

description: Failure of current chemotherapeutic drugs leads to the recurrence of tumors and increased mortality in lung cancer patients. This study investigated 4,5,4'-trihydroxy-3,3'-dimethoxybibenzyl (TDB), a bibenzyl extracted from Dendrobium ellipsophyllum Tang and Wang as a novel anticancer agent for lung cancer. Selective anticancer activity of TDB against human lung cancer cells was demonstrated with a high half maximal inhibitory concentration (IC50) approximately &gt; 300 µM in dermal papilla cells, while IC50 in human lung cancer H460 cells was approximately 100 ± 5.18 µM. Flow cytometry confirmed that TDB (50 µM) treatment for 24 h, caused significant early and late apoptosis but not necrosis in H460 cells. The up-regulation of p53, a tumor-suppressor protein, was observed in human lung cancer cells treated with 10-50 µM of TDB. The mechanism for apoptosis was attributed to p53 activation, which was related to up-regulation of pro-apoptotic Bax, reduction of anti-apoptosis Bcl-2 and Mcl-1 proteins and suppression on protein kinase B (Akt) survival pathway. Another possible mechanism involved in TDB-induced apoptosis in H460 cells was the modulation of reactive oxygen species (ROS), as intracellular ROS level was decreased by TDB (50 µM) treatment for 3-6 h as detected by 2',7'-dichlorofluorescein diacetate (DCFH2-DA) fluorescent probe. Moreover, Hoechst33342 staining showed that apoptosis was significantly decreased in H460 cells incubated with 100 µM of hydrogen peroxide (H2O2) for 30 min prior exposure to TDB (50 µM) compared with the cells only treated with TDB. Additionally, TDB inhibited cancer stem cell (CSC)-like characteristics in H460 cells as evidenced in spheroid assay and flow cytometry analysis, whereby CSCs, CD133-positive cells markedly decreased after TDB treatment. TDB caused a decrease in protein levels of the stemness-related transcription factors; Nanog and Sox2 while there was no alteration of Oct4. There was the reduction of related regulator proteins, β-catenin. In conclusion, TDB extracted from D. ellipsophyllum demonstrated stemness suppression and selective apoptosis induction via intracellular ROS reduction in human lung cancer cells. The results obtained from this study strengthen the potential development of TDB as an anticancer compound with a favorable safety profile.
DOI: 10.14457/cu.the.2018.78
title: Thai civil partnership is the new marriage inequality : queer critiques on the discourses in the civil partnership bill of state activism towards marriage ‘equality’

description: With an equal access to legal registration becoming the international mainstream development agenda and movement of 21st century, Thailand proposed a civil partnership bill developed by the Rights and Liberties Protection Department in 2018. Although the initiative seems promisingly progressive, the drafting, legal product and outcomes that the bill promises to bring lay vigorous ground of criticisms. This thesis is the first English-language academic work that uniquely integrates theoretical outlook and arguments of queer theory where Foucauldian discourse analysis is the key concept applied to critique the discourses codified in the bill. This thesis aims to analyze how Thai discourse of marriage affects state activism of marriage equality through the proposal of controversial civil partnership and argue how it is a new marriage inequality. This thesis presents key findings derived from secondary sources, content analysis of the bill, interviews with key actors, participation in relevant activities and events and insights from the author as an activist. The discourse of marriage in Thailand is more social and cultural than legal where witnessed ceremony without registration with state is regarded as recognition. Interacting with foreign influences, the civil partnership as a new registration system was proposed to make marriage more gender-inclusive and arguably equal, however the criticisms prove otherwise. Thai movement of marriage equality faces internal complexity among the civil society and state authorities. This paper concludes that beyond the limitation of rights, the civil partnership bill is a new marriage inequality. The oppressive institution of marriage whose discourse is monopolized by state is used as a tool to control and discipline Thai LGBTI couples. The discriminative civil partnership act normalizes and compromises the needs of the community with statist interest of state, enabling the reproduction and idealization of state-approved norms and expectations.
DOI: 10.14457/cu.the.2018.79
title: Women political participation in Myanmar’s house of representatives

description: The thesis mainly discusses about main obstacles and challenges for women to participate in political activities in Myanmar including economic factor, social factor, political factor, and the ways how women are empowered to participate in political activities in Myanmar by using both primary and secondary data. The primary concept for this study is based on women empowerment processes which are included four different pathways in which women are changed –material (economy), perceptual (knowledge and skill), cognitive (self-confident and self-esteemed) and relational (bargaining power). Moreover, the thesis analyses actor’s roles in these four pathways. The thesis found out external factors namely economy, relgion, culture and tradition, verbal gender-discrimination and gender-violence as well as lack of gender-concept in policy and poltiical factor are obstacles and challenges for women. On the other hand, internal factors namely education, knowledge and skills and self-confident are not challenges for women to participate in poltiical activities. In order to empower women, there are four main actors in Myanmar: state (Ministry of Social Welfare, Union Election Commission), political parties, CSO/NGO/INGO and media for four pathways. It is observed that for four pathways, state and political parties takes cooperation and leading roles for economic (material pathway), knowledge and skills (cognitive pathway) and self-confident (perceptual pathway) of women empowerment with CSO/NGO/INGO sector. But state cannot do its cooperation and leading role very well like political parties. The thesis found out CSO/NGO/INGO can do a lot for women empowerment. Without support of CSO/NGO/INGO, state and political parties cannot do anything for material, cognitive and perceptual pathways. And for bargaining power (relational pathway), CSO/NGO/INGO does not have power to do. State and Political parties have power but in reality, state and political parties cannot do anything for bargaining power for women. For media sector, the thesis found out it can just support for improving knowledge for women.
DOI: 10.14457/cu.the.2018.80
title: Effects of mutations of levansucrase from Bacillus licheniformis RN-01 on levan oligosaccharides binding

description: Produced by levansucrase that uses sucrose as a substrate, levan and levan oligosaccharides (GFn) have potential applications in food and pharmaceutical industries such as prebiotics, anti-obesity and anti-tumor agents. Previous study reported that N251A and N251Y mutants of levansucrase from Bacillus licheniformis RN-01 could effectively produce short-chain oligosaccharides upto GF3, instead of long-chain levan synthesis. This study hypothesized that these mutations probably reduced GF3 binding affinity in the active site of levansucrase that contains fructosyl-Asp93 intermediate and caused GF3 to be in an unfavorable orientation for transfructosylation; therefore, levansucrase could not effectively extend GF3 to GF4. However, these mutations probably did not significantly reduce the binding affinity or drastically change orientation of GF2; therefore, levansucrase could still extend GF2 to GF3. To test this hypothesis, this study employed molecular dynamics to investigate effects of these mutations on GF2/GF3 binding in the active site of levansucrase. The results reasonably support this hypothesis as N251A and N251Y mutations did not significantly reduce the GF2 binding affinity and did not drastically change orientation of GF2 in the active site of levansucrase. However, these mutations drastically decreased GF3 binding affinity and caused GF3 to be in an unfavorable orientation for transfructosylation. Furthermore, the free energy decomposition and hydrogen bond occupation results suggest the importance of Arg255 in GF2/GF3 binding in the active site of levansucrase. To elucidate the importance of Arg255 on the production of levan, R255A mutant of levansucrase was successfully cloned and expressed. The optimum pH and temperature of R255A mutant was at 6.0 and 50 oC, respectively. This study found that R255A mutant could not synthesize long-chain levan, but it synthesized a small amount of short-chain levan oligosaccharides with the chain length upto GF2, supporting the computational results. This knowledge could be beneficial in designing levansucrase to efficiently produce levan oligosaccharides with desired length.
DOI: 10.14457/cu.the.2018.81
title: Development and screening of chalcones against cancer protein targets using in silico and in vitro techniques

description: Targeted cancer therapy has become one of high potential cancer treatments. Human topoisomerase IIα (hTopoIIα) and Epidermal growth factor receptor tyrosine kinase (EGFR-TK) are proteins that play important roles on cell cycle. The hTopoIIα catalyzes the cleavage and rejoining of double-stranded DNA while EGFR function on cell signaling. Thus, the two proteins have been suggested as molecular target for the development of novel cancer therapeutics. A series of 47 chalcone derivatives was screened against hTopoIIα and EGFR-TK by in silico and in vitro techniques. Chalcone 3d showed a high cytotoxicity with IC50 values of 10.8, 3.2 and 21.1 µM against the HT-1376, HeLa and MCF-7 cancer cell lines, respectively, while 1c, 2a, 3e, 4e and 4t exhibited potent compounds against A431 with IC50 values &lt; 10 µM. To confirm the result the inhibition of those chalcone on enzyme assay was obtained. The 3d showed an inhibitory activity against hTopoIIα that was better than the known inhibitor, salvicine. Only three chalcones (1c, 2a and 3e) had an inhibitory activity against EGFR-TK with relative inhibition percentages closed to the approved drug, erlotinib. The binding interaction of potent chalcones complex with hTopoIIα and EGFR-TK were studied using molecular dynamics simulation. The observed 3d/hTopoIIα interactions affirmed that 3d strongly interacts with the ATP binding pocket residues while 1c, 2a and 3e/EGFR-TK were well occupied within the ATP binding site and strongly interacted with the 7 hydrophobic residues including the important hinge region residue M793. From ADMET properties, all six chalcones could be served as a lead compounds for development of hTopoIIα and EGFR-TK inhibitors. The information of drug development can be obtained by quantitative structure-activity relationship (QSAR) techniques. A series of chalcone derivatives against A431 were subjected to QSAR analyses. The MLR equation indicated a good correlation between observed and predicted values with R2 = 0.968. The understanding of the relationship between structures and pharmacological effects and the ligand-protein binding pattern leads to important information for the design of high potential anti-cancer drugs.​
DOI: 10.14457/cu.the.2018.82
title: Electrochemical detection of herbicides and antioxidants using nanomaterial-modified electrode

description: This dissertation focused on the development of a novel nanomaterial-based electrochemical sensors for pharmaceutical and environmental applications. The research is divided into two parts. In the first part, a low-cost and disposable sensor for the simultaneous determination of coenzyme Q10 (CoQ10) and α-lipoic acid (ALA) using manganese (IV) oxide-modified screen-printed graphene (MnO2/SPGE) electrode was developed. The incorporated MnO2 in the SPGE can greatly enhance the detection performance towards the oxidation of CoQ10 and ALA. The cyclic voltammetry (CV) and square-wave anodic stripping voltammetry (SWASV) were employed to investigate the electrochemical behavior of the developed sensor and also to perform the quantitative analysis of the analytes. This developed sensor provides an inexpensive and disposable sensor for the detection of CoQ10 and ALA. Furthermore, the nanomaterial-based electrochemical sensor exhibits the potential to integrate with other analytical methods for improving the analytical performance. The detail was demonstrated in part 2. In this part, the gold nanoparticle-modified screen-printed carbon electrode (AuNP/SPCE) integrated with ultra-high performance liquid chromatography (UHPLC) was developed for the detection of dithiocarbamate herbicides. Based on the obtained results, AuNPs play a catalytic activity towards the oxidation of dithiocarbamate compounds. The amperometric detection was performed after separation using UHPLC technique. The experimental parameters of both researches were optimized to obtain the best condition. The sensitivity, selectivity and analytical performance of both proposed sensors were discussed. Under the optimal conditions, the proposed sensors showed an acceptable range of accuracy and precision with low limits of detection in the micro-gram levels. Finally, the proposed sensors were successfully applied to detect target analytes in pharmaceutical, fruit, and vegetable samples. These developed systems exhibit the high potential to be useful methodologies for practical applications.
DOI: 10.14457/cu.the.2018.83
title: Characterization of PmSpӓtzle and SPIPm5 from black tiger shrimp Penaeus monodon

description: Humoral response proteins in shrimp, antimicrobial proteins (AMPs) and proteinase inhibitors, are implicated in host defenses against the pathogen infection because their expression is changed in response to microbial infection. After the pathogen infection in shrimp, the Spätzle protein is synthesized and activated the pathogenic signaling via Toll pathway by its active protein for the synthesis of AMPs. In this study, three PmSpätzle isoforms, PmSpz1, 2 and 3 genes, were identified in Penaeus monodon. The PmSpz1 gene was chosen for studied in details. Its gene was expressed in all shrimp tissues tested and in response to WSSV infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as an active form awaiting to activate Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV challenge. The recombinant of active Spätzle activated the synthesis of AMP genes: crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5. It is indicating that the expression of all antimicrobial proteins but not penaeidin5 was expressed under the regulation of Toll pathway. For SPIPm5 protein, the Kazal-type serine proteinase inhibitor from P. monodon was also studied. Its transcripts were also expressed in all tissues tested and its gene expression was up-regulated by heat stress, WSSV and YHV. Injection of rSPIPm5 into shrimp to mimic heat stress condition had more or less no effect on the crustinPm1, penaeidin3, penaeidin5, Hsp70, SPIPm2 and SPIPm5. The effect of rSPIPm5 protein inhibited the hemolymph proPO activity. In survival experiments, the rSPIPm5 could also prolong the shrimp from pathogen infection, WSSV, YHV, Vibrio harveyi and Vibrio parahaemolyticus in shrimp. The increased endurance against the microbial infection was due to the inhibitory effects presumably activated by rSPIPm5 on viral replication and bacterial growth but not the expression of AMPs gene. The microbial inhibitory effects may be activated by rSPIPm5. Therefore, the SPIPm5 plays an important role in shrimp innate immunity against the viral and bacterial infection besides its function as a proteinase inhibitor.