cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/qmf64-kr580
title: HUVECs 24h Infx pNLgfp

description: HUVECs infected by spinoculation with VSVG-pNL4GFP, frozen 24 h after synchronized entry
description: <strong>Tilt Series Date:</strong> 2011-03-31</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Cells infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-03-31-37/3dimage_68473/</p>11mar31a__050_full.rec,  Reconstruction (Pixel Size 2.0 nm), 4.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-03-31-37/3dimage_68473/11mar31a__050_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11mar31a__050.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-03-31-37/rawdata/11mar31a__050.st"
        > <i class="download icon"></i> Download </a></p>

        11mar31a__050_medflt.tiff,  file associated to 3D Image #68473, 947.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-03-31-37/file_15912/11mar31a__050_medflt.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rcz0k-brh75
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns_full.jpg: file associated to 3D Image #54393
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-22/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns.st,  Tilt Series (Pixel Size 0.5 nm), 858.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-22/rawdata/11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 578.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-22/3dimage_54393/11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns_full.tiff,  file associated to 3D Image #54393, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-22/file_13123/11dec12_cryosec_pnlegfp_grid3_Batch5_0039_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3jtvv-42r86
title: liposomes + 1uL CdvA

description: tetraether liposomes from Sulfolobus cells + 1uL CdvA
description: <strong>Tilt Series Date:</strong> 2011-07-29</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> tetraether liposomes + CdvA</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 1°, constant angular increment, dosage: 8.0 eV/Å²,  defocus: -5.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> sent by Rachel Samson at Oxford University, purified by Parkson Lee-Gau Chong at Temple University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> heavy vortexing for 10 mins at 80 C</p>
<strong>Sample Preparation:</strong> prepared by Parkson Lee-Gau Chong</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-07-29-5/rawdata/</p>liposomes1uLcdvA_27500x0005.mrc,  Tilt Series (Pixel Size 7.74 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-07-29-5/rawdata/liposomes1uLcdvA_27500x0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        liposomes1uLcdvA_27500x0005_full.rec,  Reconstruction (Pixel Size 1.5 nm), 353.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-07-29-5/3dimage_19355/liposomes1uLcdvA_27500x0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        liposomes1uLcdvA_27500x0005.rec,  Reconstruction (Pixel Size 1.5 nm), 176.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-07-29-5/3dimage_19356/liposomes1uLcdvA_27500x0005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mbfgp-2ct75
title: liposomes + 1uL CdvA

description: tetraether liposomes from Sulfolobus cells + 1uL CdvA
description: <strong>Tilt Series Date:</strong> 2011-08-02</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> tetraether liposomes + CdvA</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 1°, constant angular increment, dosage: 8.0 eV/Å²,  defocus: -5.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> sent by Rachel Samson at Oxford University, purified by Parkson Lee-Gau Chong at Temple University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> heavy vortexing for 10 mins at 80 C</p>
<strong>Sample Preparation:</strong> prepared by Parkson Lee-Gau Chong</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-02-4/rawdata/</p>liposomes1uLcdvA_27500x0017.mrc,  Tilt Series (Pixel Size 7.74 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-02-4/rawdata/liposomes1uLcdvA_27500x0017.mrc"
        > <i class="download icon"></i> Download </a></p>

        liposomes1uLcdvA_27500x0017_full.rec,  Reconstruction (Pixel Size 1.5 nm), 295.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-02-4/3dimage_19425/liposomes1uLcdvA_27500x0017_full.rec"
        > <i class="download icon"></i> Download </a></p>

        liposomes1uLcdvA_27500x0017.rec,  Reconstruction (Pixel Size 1.5 nm), 147.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-08-02-4/3dimage_19426/liposomes1uLcdvA_27500x0017.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p4rht-46w71
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-12-25</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-25-7/rawdata/</p>11dec25_C104_2_COS7_4ADN_AT1BT10008_ns.st,  Tilt Series (Pixel Size 0.5 nm), 900.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-25-7/rawdata/11dec25_C104_2_COS7_4ADN_AT1BT10008_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec25_C104_2_COS7_4ADN_AT1BT10008_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 588.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-25-7/3dimage_68151/11dec25_C104_2_COS7_4ADN_AT1BT10008_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec25_C104_2_COS7_4ADN_AT1BT10008_ns_full.tiff,  file associated to 3D Image #68151, 1.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-25-7/file_15866/11dec25_C104_2_COS7_4ADN_AT1BT10008_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.bt93ut
title: Occurrence Download

description: A dataset containing 5876 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acer negundo",
 "Country is Ukraine",
 "HasCoordinate is true"
 ]
}

 The dataset includes 5876 records from 94 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046646-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/gd01j-wh139
title: HUVECs VPS4DN+pNLGFP

description: HUVECs cells transfected with VPS4DN construct and then infected with pNLEGFP-VSVG. Sample#C68
description: <strong>Tilt Series Date:</strong> 2011-07-24</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP proviral construct from Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward. Cells transfected and infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HUVECs grown on grids and transfected with VPS4DN and then infected 24 later with pNLGFP-VSVG</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-24-12/rawdata/</p>11jul24_HUVECS_DNpnlegfp_21.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-24-12/rawdata/11jul24_HUVECS_DNpnlegfp_21.st"
        > <i class="download icon"></i> Download </a></p>

        11jul24_HUVECS_DNpnlegfp_21_trim_full.rec,  Reconstruction (Pixel Size 2.0 nm), 681.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-24-12/3dimage_68360/11jul24_HUVECS_DNpnlegfp_21_trim_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11jul24_hela_DNpnlegfp_21_trim_full.tiff,  file associated to 3D Image #68360, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-24-12/file_15881/11jul24_hela_DNpnlegfp_21_trim_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hc0gr-sm911
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky. Used for CLEM
description: <strong>Tilt Series Date:</strong> 2011-12-15</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.jpg: file associated to 3D Image #54418
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-1/rawdata/</p>11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns.st,  Tilt Series (Pixel Size 0.5 nm), 941.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-1/rawdata/11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-1/3dimage_54418/11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.tiff,  file associated to 3D Image #54418, 2.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-1/file_13149/11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kzr9b-gkn58
title: HUVECs infected w/ pNLGFP

description: HUVECs cells infected with pNLGFP and cultured additional 24 h in presence of protease inhibitor. Sample# C71
description: <strong>Tilt Series Date:</strong> 2011-06-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct from Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward. Cells infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HUVECs grown on grids and infected pNLGFP-VSVG and then cultures additional 24 h allowed virus expression in presence of protease inhibitor </p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-4/rawdata/</p>11jun04_PI_4.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-4/rawdata/11jun04_PI_4.st"
        > <i class="download icon"></i> Download </a></p>

        11jun04_PI_4_full.rec,  Reconstruction (Pixel Size 2.0 nm), 483.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-4/3dimage_68368/11jun04_PI_4_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11jun04_PI_4.tiff,  file associated to 3D Image #68368, 659.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-4/file_15886/11jun04_PI_4.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cth4x-gmn82
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns_full.jpg: file associated to 3D Image #54381
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-16/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns.st,  Tilt Series (Pixel Size 0.5 nm), 933.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-16/rawdata/11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 408.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-16/3dimage_54381/11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns_full.tiff,  file associated to 3D Image #54381, 1.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-16/file_13113/11dec12_cryosec_pnlegfp_grid3_Batch4_0032_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w41sq-71h46
title: HeLa siRNA tfx CHMP2A/B infx pNLGFP

description: HeLa cells infected with pNLGFP and 12h post infection cells were transfected with siRNA targeting CHMP2A and CHMP2B. 18h after siRNA tfx, cells were frozen Sample# C85
description: <strong>Tilt Series Date:</strong> 2011-06-27</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP2A and 2B siRNAs provided by Wes Sundquist. pNLGFP proviral construct provided by Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward.  Cells transfected, infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and infected and siRNA transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-27-6/rawdata/</p>11Jun27_346_C85_2_0011_new.st,  Tilt Series (Pixel Size 0.5 nm), 726.7 MB  
        
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        11Jun27_346_C85_2_0011_new_full.rec,  Reconstruction (Pixel Size 2.0 nm), 103.2 MB  
        
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        11Jun27_346_C85_2_0011_new_full.tiff,  file associated to 3D Image #68385, 1.0 MB  
        
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