cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j01r6qh1
title: CIL:36299, Rattus, astrocyte. In Cell Image Library

description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 3 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow (green) and Alexa 568 (red) Optical sections were generated with a single photon confocal microscope.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow; Alexa Fluor 568
Data qualification: Processed;spatialmeasurements
description: Male Sprague Dawley rats, 3 weeks of age, were anaesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy,cells with a small diameter soma (approx. 7–11µm) in the stratum
radiatum of CA1 were located and impaled with glass micropipettes filled with either 5% aq. LY, 20mM AlexaFluor 568 in 200mM KCl, or 20mM AlexaFluor 488 in 200mM KCl. The astrocytes were filled using a 0.5 Hz pulses of
current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000
(Hercules, CA) attached to a Nikon E600FN
(Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60× oil objective (NA 1.4). Optical z resolution, 0.3 µm. Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
DOI: 10.6075/j0m044bp
title: CIL:36288, Vorticella microstoma, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: This freeze fracture view of the pellicle of V. microstoma near the aboral end of the zooid shows the region where the width of each circumferential alveolus is narrower and thus the strings of fractured pits are closer together. In this case the E-fracture face of the outer alveolar membrane is shown which has fewer IMPs than the P-face of the outer alveolar membrane as seen in the previous figure. Here the connections are indentations rather than fractured necks. In a few areas the P-fracture face of the plasma membrane is viewed where it was pinched into the folds between rings that surround the cell. The plasma membrane, like a similar view in figure 23, demonstrates the large number of prominent IMPs on this face of the plasma membrane. large arrow, direction of shadowing of freeze fracture replica. EM taken on 11/11/75 by R. Allen with JEM 100B operating at 80kV. Neg. 21,578X. Bar = 0.20µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. There is a high resolution version of this image in the library (CIL:39326) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0f18zqv
title: CIL:36296, Rattus, astrocyte. In Cell Image Library

description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 3 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow (green) and Alexa 568 (red) Optical sections were generated with a single photon confocal microscope.
description: Male Sprague Dawley rats, 3 weeks of age, were anaesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy,cells with a small diameter soma (approx. 7–11µm) in the stratum
radiatum of CA1 were located and impaled with glass micropipettes filled with either 5% aq. LY, 20mM AlexaFluor 568 in 200mM KCl, or 20mM AlexaFluor 488 in 200mM KCl. The astrocytes were filled using a 0.5 Hz pulses of
current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000
(Hercules, CA) attached to a Nikon E600FN
(Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60× oil objective (NA 1.4). Optical z resolution, 0.2 µm. Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow; Alexa Fluor 568
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0xg9q2n
title: CIL:36286, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: The scopula, stalk-forming region, is outlined by a circle of basal bodies that produce short modified cilia. Other basal bodies lie within this circle. The sheath is secreted outside the circle of cilia. Fibers from linkage complexes enter the proximal ends of these basal bodies. T
 EM taken on 2/24/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39508) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.15468/dl.5uxutj
title: Occurrence Download

description: A dataset containing 459 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-80.395 30.81778,-63.05777 30.81778,-63.05777 45.77091,-80.395 45.77091,-80.395 30.81778))",
 "HasGeospatialIssue is false",
 "TaxonKey is Ruppia maritima"
 ]
}

 The dataset includes 459 records from 41 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009446-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j09w0d96
title: CIL:36016, Rattus, CNS neuron (sensu Vertebrata), Purkinje cell. In Cell Image Library

description: Figure 398 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Excitatory synapses on dendritic spines of a Purkinje cell from the rat cerebellum. Axon terminals from parallel fibers can be seen making synaptic contacts on the heads of the spines. Image from Peters, Palay and Webster, Fine Structure of the Nervous System, Philadelphia, W.B. Saunders Co. 1976. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j07w6b1f
title: CIL:36008, Homo sapiens, epithelial cell of pancreas. In Cell Image Library

description: Figure 390 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Secretory granules of an alpha cell (upper part of the micrograph) and two beta cells (lower) from an islet of the endocrine pancreas. Image by Arthur Like. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.57760/sciencedb.27707
title: Comprehensive Dataset on China's PM2.5 Emissions and Air Quality 2013-2020
title: Comprehensive Dataset on China's PM2.5 Emissions and Air Quality 2013-2020

description: This dataset provides a detailed account of China's PM2.5 emissions and air quality from 2013 to 2020, developed to assess the impact of national clean air policies. It includes emission inventories across six sectors for key pollutants, simulated PM2.5 concentrations using the WRF/CMAQ model, and quantified emission reductions from specific control measures. Additionally, it offers health impact assessments of PM2.5 reductions, such as avoided premature deaths. The dataset supports research in environmental science, public health, and policy analysis, enabling studies on air pollution dynamics, health outcomes, and policy effectiveness. The data is publicly accessible through the MEIC model portal and Figshare repository, providing a valuable resource for understanding and addressing air pollution challenges in China.
description: This dataset provides a detailed account of China's PM2.5 emissions and air quality from 2013 to 2020, developed to assess the impact of national clean air policies. It includes emission inventories across six sectors for key pollutants, simulated PM2.5 concentrations using the WRF/CMAQ model, and quantified emission reductions from specific control measures. Additionally, it offers health impact assessments of PM2.5 reductions, such as avoided premature deaths. The dataset supports research in environmental science, public health, and policy analysis, enabling studies on air pollution dynamics, health outcomes, and policy effectiveness. The data is publicly accessible through the MEIC model portal and Figshare repository, providing a valuable resource for understanding and addressing air pollution challenges in China.
DOI: 10.6075/j0b856xs
title: CIL:36011, Anura, myelinating Schwann cell, muscle cell, peripheral neuron. In Cell Image Library

description: Figure 393 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Longitudinal section of frog neuromuscular junction, prepared by rapid freezing with liquid helium followed by freeze substition. The nerve terminal is densely packed with synaptic vesicles, and surrounded by the myelinating Schwann cell. Image by John Heuser and Tom Reese. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j03j3br1
title: CIL:36012, Anura, peripheral neuron, muscle cell, myelinating Schwann cell. In Cell Image Library

description: Figure 394 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. "Omega figures" capturing synaptic vesicle exocytosis at the synaptic junction. This event was observed during chemical stimulation of the frog neuromuscular junction, prepared by rapid freezing with liquid helium followed by freeze substition. 4-aminopyridine was used to trigger release of the neurotransmitter acetylcholine.Image by John Heuser and Tom Reese. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j0bp01nc
title: CIL:36009, Rattus, mast cell. In Cell Image Library

description: Figure 391 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Secretory granules of a mast cell from rat connective tissue. Secretory granules in this cell type are distributed throughout the cell, and can be secreted anywhere on the cell surface. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j0m32tkc
title: CIL:36045, Petromyzon marinus, Mauthner neuron. In Cell Image Library

description: Figures 408 (upper) and 409 (lower) from Chapter 16 (Cytoplasmic matrix and cytoskeleton) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Upper panel: Vesicles, presumably in transport along cytoplasmic microtubules in a giant axon from the central nervous system of the lamprey. Lower panel: vesicles are also clustered around microtubules (arrows) at the periphery of sites of synaptic contact. Figures from Smith, J√§rlfors, Ber√°nek, J. Cell Biol. 46:199-219, 1970 (PMID:5452412). A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment; isolated subcellular component
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: electron-dense stain
Data qualification: Processed
DOI: 10.6075/j0zk5fgj
title: CIL:36015, Rattus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Figure 397 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Axosomatic and axodendritic synapses in the central nervous system of the rat. A nerve terminal in the center of the micrograph can be seen making contact with a cell body (occupying the lower right; arrows indicate two regions of apparent active zone contact. Notably, there is also synaptic contact by same axon with a dendrite to the left. A second axodendritic synapse is present at the top of the micrograph. Image by Enrico Mugnaini. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.5281/zenodo.17491200
title: Study sites of project FIREPATHS

description: Vector layers outlining the location of the study sites of project FIREPATHS obtained from project SERGISAT (CGL2014-57013-C2-2-R). incendios.gpkgoutlines the fire scars burned in 1994, buffer_incendios.gpkg outlines the neigboring region to allocate control plots; parcelas.gpkg indicates the location of the surveyed plots (see https://zenodo.org/records/17476516).
description: Capas vectoriales que delinean la ubicación de los sitios de estudio del proyecto FIREPATHS obtenidas del proyecto SERGISAT (CGL2014-57013-C2-2-R). incendios.gpkg delinea las cicatrices de incendio quemadas en 1994, buffer_incendios.gpkg delinea la región vecina para asignar parcelas de control; parcelas.gpkg indica la ubicación de las parcelas muestreadas (véase https://zenodo.org/records/17476516).
DOI: 10.5281/zenodo.17491201
title: Study sites of project FIREPATHS

description: Vector layers outlining the location of the study sites of project FIREPATHS obtained from project SERGISAT (CGL2014-57013-C2-2-R). incendios.gpkgoutlines the fire scars burned in 1994, buffer_incendios.gpkg outlines the neigboring region to allocate control plots; parcelas.gpkg indicates the location of the surveyed plots (see https://zenodo.org/records/17476516).
description: Capas vectoriales que delinean la ubicación de los sitios de estudio del proyecto FIREPATHS obtenidas del proyecto SERGISAT (CGL2014-57013-C2-2-R). incendios.gpkg delinea las cicatrices de incendio quemadas en 1994, buffer_incendios.gpkg delinea la región vecina para asignar parcelas de control; parcelas.gpkg indica la ubicación de las parcelas muestreadas (véase https://zenodo.org/records/17476516).
DOI: 10.6075/j070807s
title: CIL:36013, Petromyzon marinus, Mueller cell. In Cell Image Library

description: Figure 395 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Section through a portion of axon of a Muller cell and adjacent neuropil from the central nervous system of an ammocoete (larva) of the lamprey Petromyzon marinus. Image from Smith,D.S., Jarlfors, U., Beranek, R. J. (1970) Cell Biol. 46:199, PMID:5452412. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j0c8284g
title: CIL:35991, Rana pipiens, ectodermal cell. In Cell Image Library

description: Figure 370 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Ectoderm cell from a blastula of Rana pipiens. This cell shows a number of yolk platelets, lipid droplets and many small pigment granules. The higher magnification inset panel shows a lattice pattern in one of the yolk crystals. Image by S.J. Karaseki, J. Cell Biol. 18:135, 1963. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j0v69hd8
title: CIL:35990, Felis catus, erythrocyte. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 369 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Erythrocytes in a longitudinal section through a capillary from the myocardium of a cat. In the upper cell, the hemoglobin has formed a large single crystal, and the surrounding cytoplasm is pale. In the lower erythrocyte, hemoglobin appears to be normally distributed, and the cytoplasmic matrix appears uniform. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j00g3j01
title: CIL:35992, Campylenchia latipes, ciliated epithelial cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 371 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Ferritin crystal in midgut cell of the widefooted treehopper Campylenchia latipes. Crystallization of ferritin occurs in some insects in the intestinal tract. Image by J. Gouranton and R. Folliot. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
 
 widefooted treehopper
DOI: 10.6075/j07943gr
title: CIL:35987, Homo sapiens, Leydig cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 366 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Intranuclear crystals of Reinke in a human Leydig cell. Image courtesy of Wolf Fahrenbach. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.