DOI: 10.5061/dryad.rbnzs7hhj
title: Adult-born granule cells modulate CA2 network activity during retrieval of developmental memories of the mother

description: Adult-born granule cells (abGCs) project to the CA2 region of the
 hippocampus, but no previous studies have assigned a behavioral function
 to this circuit. Here we show that abGC input to the CA2 is necessary for
 the retrieval of remote social memories. We also found that abGCs are
 necessary for differential CA2 network activity, including theta-gamma
 coupling and sharp wave-ripples, in response to novel versus familiar
 social stimuli.
description: # Electrophysiology recordings (CA2): --- The Electrophysiology.zip file
 contains raw data obtained from wireless hippocampal recordings in freely
 behaving mice. In one experiment, Nestin-cre and Nestin-cre:Gi mice were
 recorded at baseline and during exposure to a social stimulus. In another
 experiment, Nestin-cre and Nestin-cre:Gi mice were recorded at baseline
 and during exposure to a non-social stimulus. For both experiments,
 animals were recorded after vehicle or CNO administration. Drug
 administration was counterbalanced, as was the order of stimulus
 presentation. For the social stimulus experiment, recordings occurred when
 Gi-DREADD+ adult-born neurons were 4-6 weeks old and once again when the
 cohort of neurons was 10-12 weeks old. For the non-social stimulus
 experiment, recordings occurred when Gi-DREADD+ adult-born neurons were
 4-6 weeks old only. Data filtering and normalization methods, as well as
 Python scripts used for analysis, are provided in the manuscript. Files
 were originally saved as .nex files before being converted to .mat files.
 Associated files are stored in the Electrophysiology.zip file. This zip
 file contains a folder called "Ephys recordings" which contains
 the following files: 4-6_wk_MSM_LFP - contains CA2 recordings from mice
 with Gi-DREADD+/- 4-6-week-old-adult-born neurons during social
 investigation 4-6_wk_MSM_LFP_object - contains CA2 recordings from mice
 with Gi-DREADD+/- 4-6-week-old-adult-born neurons during object
 investigation 10-12_wk_MSM_LFP - contains CA2 recordings from mice with
 Gi-DREADD+/- 10-12-week-old-adult-born neurons during social investigation
 Ephys_decoded.docx - contains information to identify groups from LFP
 files. Code: For remote social memory trials, all file names end with
 three letters (vtb, zuv, etc). Letter #1: v = Nestin-cre, z =
 Nestin-cre:Gi\ Letter #2: t = mother stimulus, u = novel mother stimulus
 Letter #3: v = male, b = female For non-social object trials, all file
 names end with three letters (vob, zov, etc). Letter #1: v = Nestin-cre, z
 = Nestin-cre:Gi Letter #2: o = object trial Letter #3: v = male, b =
 female For each trial there are four separate files (ending in \_1, \_2,
 \_3, and \_4). These files contain recordings from the corresponding
 electrode implanted in CA2 (each animal had 4 electrode wires chronically
 implanted in CA2). File names contain either "Veh" or
 "CNO" to indicate whether vehicle or Clozapine N-oxide were
 delivered, respectively. Once opened, each matlab file has three columns.
 Column 1: Time (seconds) Column 2: Signal (µV) Column 3: Blank # Histology
 The Histology.zip file contain images of the dorsal hippocampus used for
 analysis in the manuscript. Sections were stained with RGS14, PCP4,
 3R-Tau, and Znt3 antibodies. Files can be opened using Fiji (ImageJ).
 Histology.zip contains a folder called "Histology" with the
 following files: CD1 and GFAP-TK Recovery tissue - Hippocampus images from
 CD1 and GFAP-TK animals six weeks after VGCV cessation. GFAP-TK VGCV+
 tissue - Hippocampus images from GFAP-TK mice after VGCV administration.
 Code.xlsx - contains two tabs: 1) CD1 + GFAP-TK Recovery and 2) GFAP-TK
 VGCV. Tabs decode sex, strain, and condition for animals used  for
 histological analyses.