cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19752374.v1
title: Role of tRNA derived fragments in renal ischemia–reperfusion injury

description: Ischemia–reperfusion injury (IRI) is one of the major causes of acute kidney injury (AKI). tRNA derived fragments (tRFs/tiRNAs) are groups of small noncoding RNAs derived from tRNAs. To date, the role of tRFs/tiRNAs in renal IRI has not been reported. Herein, we aimed to investigate the involvement of tRFs/tiRNAs in the occurrence and development of ischemia–reperfusion-induced AKI. Moderate/severe renal IRI mouse models were established by bilateral renal pedicle clamping. The tRF/tiRNA profiles of healthy controls and moderate/severe IRI-stressed kidney tissues were sequenced by Illumina NextSeq 500. Candidate differentially expressed tiRNAs were further verified by RT-qPCR. Biological analysis was also performed. Overall, 152 tRFs/tiRNAs were differentially expressed in the moderate ischemic injury group compared with the normal control group (FC &gt; 2, <i>p</i> &lt; 0.05), of which 47 were upregulated and 105 were downregulated; in the severe ischemic injury group, 285 tRFs/tiRNAs were differentially expressed (FC &gt; 2, <i>p</i> &lt; 0.05), of which 157 were upregulated, and 128 were downregulated. RT-qPCR determination of eight abundantly expressed tiRNAs was consistent with the sequencing results. Gene Ontology analysis for target genes of the tRFs/tiRNAs showed that the most enriched cell components, molecular functions and biological processes were Golgi apparatus, cytoplasmic vesicles, protein binding, cellular protein localization and multicellular organism development. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these target genes were mainly involved in the natural killer cell mediated cytotoxicity pathway, citrate cycle, and regulation of actin cytoskeleton signaling pathway. Our results indicated that tRFs/tiRNAs were involved in renal IRI. These tRFs/tiRNAs may be effective partly <i>via</i> regulation of renal immunity, inflammation and metabolism processes. Candidate genes, including tiRNA-Gly-GCC-003, tiRNA-Lys-CTT-003, and tiRNA-His-GTG-002, might be potential biomarkers and therapeutic targets of ischemia–reperfusion injury-induced acute kidney injury.
DOI: 10.6084/m9.figshare.20066426
title: Transcriptional profile changes after treatment of ischemia reperfusion injury-induced kidney fibrosis with 18β-glycyrrhetinic acid

description: Chronic kidney disease (CKD) is characterized by renal fibrosis without effective therapy. 18β-Glycyrrhetinic acid (GA) is reported to have detoxification and anti-inflammatory functions and promotes tissue repair. However, the role of GA in CKD remains unclear. In this study, we investigated whether GA has a potential therapeutic effect in kidney fibrosis. A renal fibrosis mouse model was established by ischemia/reperfusion (I/R) injury via clamping unilateral left renal pedicle for 45 min; then, the mice were treated with vehicle or GA. Kidney tissues and blood samples were extracted 14 days after reperfusion and renal function, histopathological staining, quantitative PCR, and western blotting were performed. RNA-seq was performed to explore the changes in the transcriptional profile after GA treatment. Renal function, pathological and molecular analysis displayed that fibrosis was successfully induced in the I/R model. In the GA treatment group, the severity of fibrosis gradually reduced with the best effect seen at a concentration of 25 mg kg <sup>−1</sup>. A total of 970 differentially expressed genes were identified. Pathway enrichment showed that reduced activation and migration of inflammatory cells and decreased chemokine interaction in significant pathways. Protein–protein interaction networks were constructed and 15 hub genes were selected by degree rank, including chemokines, such as C3, Ccl6, Ccr2, Ptafr, Timp1, and Pf4. GA may alleviate renal fibrosis by inhibiting the inflammatory response. GA is a promising therapy that may perhaps be used in treating renal fibrosis and CKD.
DOI: 10.6084/m9.figshare.19248107
title: Acute kidney injury defined by cystatin C may be superior for predicting the outcomes of liver cirrhosis with acute gastrointestinal bleeding

description: Acute kidney injury (AKI) is conventionally evaluated by a dynamic change of serum creatinine (Scr). Cystatin C (CysC) seems to be a more accurate biomarker for assessing kidney function. This retrospective multicenter study aims to evaluate whether AKI re-defined by CysC can predict the in-hospital outcomes of patients with liver cirrhosis and acute gastrointestinal bleeding. Overall, 677 cirrhotic patients with acute gastrointestinal bleeding, in whom both Scr and CysC levels were detected at admissions, were screened. eGFR<sub>Scr</sub>, eGFR<sub>CysC</sub>, and eGFR<sub>Scr-CysC</sub> were calculated. MELD-Na score and AKI were re-evaluated by CysC instead of Scr. Odds ratios (ORs) were calculated in the logistic regression analyses. The receiver operating characteristic (ROC) curve analyses were performed. Univariate logistic regression analyses demonstrated that baseline Scr and CysC levels, eGFR<sub>Scr</sub>, eGFR<sub>CysC</sub>, eGFR<sub>Scr-CysC</sub>, original MELD-Na score defined by Scr, MELD-Na score re-defined by CysC, and AKI re-defined by CysC, but not conventional AKI defined by Scr, were significantly associated with in-hospital death. ROC analyses showed that baseline CysC level, eGFR<sub>Scr</sub>, eGFR<sub>CysC</sub>, eGFR<sub>Scr-CysC</sub>, original MELD-Na score defined by Scr, and MELD-Na score re-defined by CysC, but not baseline Scr level, could significantly predict the risk of in-hospital death. AKI re-defined by CysC may be superior for predicting the in-hospital mortality of cirrhotic patients with acute gastrointestinal bleeding.
DOI: 10.6084/m9.figshare.19248107.v1
title: Acute kidney injury defined by cystatin C may be superior for predicting the outcomes of liver cirrhosis with acute gastrointestinal bleeding

description: Acute kidney injury (AKI) is conventionally evaluated by a dynamic change of serum creatinine (Scr). Cystatin C (CysC) seems to be a more accurate biomarker for assessing kidney function. This retrospective multicenter study aims to evaluate whether AKI re-defined by CysC can predict the in-hospital outcomes of patients with liver cirrhosis and acute gastrointestinal bleeding. Overall, 677 cirrhotic patients with acute gastrointestinal bleeding, in whom both Scr and CysC levels were detected at admissions, were screened. eGFR<sub>Scr</sub>, eGFR<sub>CysC</sub>, and eGFR<sub>Scr-CysC</sub> were calculated. MELD-Na score and AKI were re-evaluated by CysC instead of Scr. Odds ratios (ORs) were calculated in the logistic regression analyses. The receiver operating characteristic (ROC) curve analyses were performed. Univariate logistic regression analyses demonstrated that baseline Scr and CysC levels, eGFR<sub>Scr</sub>, eGFR<sub>CysC</sub>, eGFR<sub>Scr-CysC</sub>, original MELD-Na score defined by Scr, MELD-Na score re-defined by CysC, and AKI re-defined by CysC, but not conventional AKI defined by Scr, were significantly associated with in-hospital death. ROC analyses showed that baseline CysC level, eGFR<sub>Scr</sub>, eGFR<sub>CysC</sub>, eGFR<sub>Scr-CysC</sub>, original MELD-Na score defined by Scr, and MELD-Na score re-defined by CysC, but not baseline Scr level, could significantly predict the risk of in-hospital death. AKI re-defined by CysC may be superior for predicting the in-hospital mortality of cirrhotic patients with acute gastrointestinal bleeding.
DOI: 10.6084/m9.figshare.20066426.v1
title: Transcriptional profile changes after treatment of ischemia reperfusion injury-induced kidney fibrosis with 18β-glycyrrhetinic acid

description: Chronic kidney disease (CKD) is characterized by renal fibrosis without effective therapy. 18β-Glycyrrhetinic acid (GA) is reported to have detoxification and anti-inflammatory functions and promotes tissue repair. However, the role of GA in CKD remains unclear. In this study, we investigated whether GA has a potential therapeutic effect in kidney fibrosis. A renal fibrosis mouse model was established by ischemia/reperfusion (I/R) injury via clamping unilateral left renal pedicle for 45 min; then, the mice were treated with vehicle or GA. Kidney tissues and blood samples were extracted 14 days after reperfusion and renal function, histopathological staining, quantitative PCR, and western blotting were performed. RNA-seq was performed to explore the changes in the transcriptional profile after GA treatment. Renal function, pathological and molecular analysis displayed that fibrosis was successfully induced in the I/R model. In the GA treatment group, the severity of fibrosis gradually reduced with the best effect seen at a concentration of 25 mg kg <sup>−1</sup>. A total of 970 differentially expressed genes were identified. Pathway enrichment showed that reduced activation and migration of inflammatory cells and decreased chemokine interaction in significant pathways. Protein–protein interaction networks were constructed and 15 hub genes were selected by degree rank, including chemokines, such as C3, Ccl6, Ccr2, Ptafr, Timp1, and Pf4. GA may alleviate renal fibrosis by inhibiting the inflammatory response. GA is a promising therapy that may perhaps be used in treating renal fibrosis and CKD.
DOI: 10.18738/t8/uoes4s/rnelfm
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/3pcmho
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/zdvtb6
title: Vid2RealPhase2_P12_vectornavIMU.txt
DOI: 10.34740/kaggle/dsv/9593733
title: Trending Youtube Video Statistics (113 Countries)
DOI: 10.18738/t8/uoes4s/mlk7du
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/z7oxyx
title: description.txt
DOI: 10.18738/t8/uoes4s/ypi9jj
title: description.txt
DOI: 10.18738/t8/uoes4s/8fvuou
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/ldqso7
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/gtsxbo
title: Vid2RealPhase2_P18_vectornavIMU.txt
DOI: 10.18738/t8/uoes4s/d3ypfn
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/hjm38m
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/vtg4r0
title: Vid2RealPhase2_P3_vectornavIMU.txt
DOI: 10.18738/t8/uoes4s/qzurt0
title: description.txt
DOI: 10.18738/t8/uoes4s/26fait
title: description.txt
DOI: 10.18738/t8/uoes4s/kidccj
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/erbeib
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/po2yiw
title: Vid2RealPhase2_P9_vectornavIMU.txt
DOI: 10.18738/t8/uoes4s/n9dwqq
title: description.txt
DOI: 10.18738/t8/uoes4s/ep6mew
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/qp0atr
title: Vid2RealPhase2_P4_vectornavIMU.txt
DOI: 10.18738/t8/uoes4s/p3yaw3
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/fjy2ts
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/xvucma
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/su8vqg
title: description.txt
DOI: 10.18738/t8/uoes4s/i9zeou
title: Vid2RealPhase2_P27_vectornavIMU.txt
DOI: 10.18738/t8/uoes4s/gdtrzg
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/uxx2jm
title: description.txt
DOI: 10.60893/figshare.sss.27057769
title: 01965-01

description: Survey
DOI: 10.60893/figshare.sss.27057769.v1
title: 01965-01

description: Survey
DOI: 10.60893/figshare.sss.27057772
title: 01965-02

description: C 1s
DOI: 10.60893/figshare.sss.27057772.v1
title: 01965-02

description: C 1s
DOI: 10.60893/figshare.sss.27057775
title: 01965-03

description: Mg 1s
DOI: 10.60893/figshare.sss.27057775.v1
title: 01965-03

description: Mg 1s
DOI: 10.18738/t8/uoes4s/h6sltx
title: timestamps.txt
DOI: 10.60893/figshare.sss.27057778
title: 01965-04

description: Mg 2s
DOI: 10.60893/figshare.sss.27057778.v1
title: 01965-04

description: Mg 2s
DOI: 10.60893/figshare.sss.27057781
title: 01965-05

description: Mg 2p
DOI: 10.60893/figshare.sss.27057781.v1
title: 01965-05

description: Mg 2p
DOI: 10.60893/figshare.sss.27057784.v1
title: 01965-06

description: Mg KLL
DOI: 10.18738/t8/uoes4s/q6nfeh
title: Vid2RealPhase2_P7_vectornavIMU.txt
DOI: 10.60893/figshare.sss.27057784
title: 01965-06

description: Mg KLL
DOI: 10.60893/figshare.sss.27057787
title: 01965-07

description: Al 2p
DOI: 10.60893/figshare.sss.27057787.v1
title: 01965-07

description: Al 2p
DOI: 10.60893/figshare.sss.27057790
title: 01965-08

description: Ti 2p
DOI: 10.60893/figshare.sss.27057790.v1
title: 01965-08

description: Ti 2p
DOI: 10.60893/figshare.sss.27057793
title: 01965-09

description: O 1s
DOI: 10.60893/figshare.sss.27057793.v1
title: 01965-09

description: O 1s
DOI: 10.18738/t8/uoes4s/ngenwh
title: timestamps.txt
DOI: 10.60893/figshare.sss.27057796
title: 01966-01

description: Survey
DOI: 10.60893/figshare.sss.27057796.v1
title: 01966-01

description: Survey
DOI: 10.60893/figshare.sss.27057799.v1
title: 01966-02

description: C 1s
DOI: 10.60893/figshare.sss.27057799
title: 01966-02

description: C 1s
DOI: 10.60893/figshare.sss.27057802
title: 01966-03

description: N 1s
DOI: 10.60893/figshare.sss.27057802.v1
title: 01966-03

description: N 1s
DOI: 10.18738/t8/uoes4s/f5b5mu
title: description.txt
DOI: 10.60893/figshare.sss.27057805
title: 01966-04

description: Mg 1s
DOI: 10.60893/figshare.sss.27057805.v1
title: 01966-04

description: Mg 1s
DOI: 10.60893/figshare.sss.27057808
title: 01966-05

description: Mg 2s
DOI: 10.60893/figshare.sss.27057808.v1
title: 01966-05

description: Mg 2s
DOI: 10.60893/figshare.sss.27057811
title: 01966-06

description: Mg 2p
DOI: 10.60893/figshare.sss.27057811.v1
title: 01966-06

description: Mg 2p
DOI: 10.18738/t8/uoes4s/mspw88
title: Vid2RealPhase2_P16_vectornavIMU.txt
DOI: 10.60893/figshare.sss.27057814
title: 01966-07

description: Mg KLL
DOI: 10.60893/figshare.sss.27057814.v1
title: 01966-07

description: Mg KLL
DOI: 10.60893/figshare.sss.27057817
title: 01966-08

description: Al 2p
DOI: 10.60893/figshare.sss.27057817.v1
title: 01966-08

description: Al 2p
DOI: 10.18738/t8/uoes4s/algacd
title: timestamps.txt
DOI: 10.60893/figshare.sss.27057820
title: 01966-09

description: Ti2p
DOI: 10.60893/figshare.sss.27057820.v1
title: 01966-09

description: Ti2p
DOI: 10.60893/figshare.sss.27057823
title: 01966-10

description: O 1s
DOI: 10.60893/figshare.sss.27057823.v1
title: 01966-10

description: O 1s
DOI: 10.15468/dl.h92rj3
title: Occurrence Download

description: A dataset containing 1109 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bromus grossus Desf. ex DC.",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 1109 records from 34 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006008-241007104925546/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.18738/t8/uoes4s/sgohvj
title: timestamps.txt
DOI: 10.18738/t8/uoes4s/oza0ua
title: description.txt
DOI: 10.18738/t8/uoes4s/f0hcbv
title: Vid2RealPhase2_P24_vectornavIMU.txt
DOI: 10.18738/t8/uoes4s/x68a58
title: timestamps.txt
DOI: 10.5281/zenodo.13886160
title: Techno-economic comparison of power-to-Ammonia and biomass- to-Ammonia plants using electrolyzer, CO 2  capture and water-gas- shift membrane reactor

description: A set of imput data used for the paper entitled: Techno-economic comparison of power-to-Ammonia and biomass-to-Ammonia plants using electrolyzer, CO 2  capture and water-gas-shift membrane reactor
DOI: 10.5281/zenodo.13886159
title: Techno-economic comparison of power-to-Ammonia and biomass- to-Ammonia plants using electrolyzer, CO 2  capture and water-gas- shift membrane reactor

description: A set of imput data used for the paper entitled: Techno-economic comparison of power-to-Ammonia and biomass-to-Ammonia plants using electrolyzer, CO 2  capture and water-gas-shift membrane reactor
DOI: 10.6084/m9.figshare.19083613.v1
title: Bioactive peptides from broccoli stems strongly enhance regenerative keratinocytes by stimulating controlled proliferation

description: As the interest on the research of plant derived bioactive peptides (BPs) for nutraceutical, cosmeceutical and medical applications is increasing, in this work, the application of peptide derived from broccoli to keratinocytes was studied. We focussed on the characterization of different peptides hydrolysates from broccoli stems [extracted from total protein (E) and from membrane protein (MF)], and their activity when applied to human keratinocytes. Peptide mixtures from broccoli stems (E and MF) were characterized by proteomics. They were applied to HaCaT cells in order to study cytotoxicity in a concentration range between 20 and 0.15625 µg of protein/mL and wound healing was studied after 24 and 48 h of treatment application. Also, proteomic and gene expression of keratinocytes were analysed. Depending on the source, proteins varied in peptide and amino acid composition. An increased proliferation of keratinocytes was shown after the application of the E peptides mixtures, reaching 190% with the lowest concentrations, but enhanced wound healing repair with E and MF appeared, reaching 59% of wound closure after 48 h. At the gene expression and protein levels of keratinocytes, the upregulation of anti-oncogene p53 and keratinization factors were observed. These results suggest that peptide mixtures obtained from broccoli augmented cell proliferation and prevented the carcinogenic, uncontrolled growth of the cells, with different mechanisms depending on the protein source. The results encourage the opening of new lines of research involving the use of <i>Brassica</i> peptides for pharmaceutic or cosmetic use.
DOI: 10.6084/m9.figshare.19806172
title: Folic acid: a potential inhibitor against SARS-CoV-2 nucleocapsid protein

description: Coronavirus disease 2019 is a global pandemic. Studies suggest that folic acid has antiviral effects. Molecular docking shown that folic acid can act on SARS-CoV-2 Nucleocapsid Phosphoprotein (SARS-CoV-2 N). To identify novel molecular therapeutic targets for SARS-CoV-2. Traditional Chinese medicine targets and virus-related genes were identified with network pharmacology and big data analysis. Folic acid was singled out by molecular docking, and its potential target SARS-CoV-2 N was identified. Inhibition of SARS-CoV-2 N of folic acid was verified at the cellular level. In total, 8355 drug targets were potentially involved in the inhibition of SARS-CoV-2. 113 hub genes were screened by further association analysis between targets and virus-related genes. The hub genes related compounds were analysed and folic acid was screened as a potential new drug. Moreover, molecular docking showed folic acid could target on SARS-CoV-2 N which inhibits host RNA interference (RNAi). Therefore, this study was based on RNAi to verify whether folic acid antagonises SARS-CoV-2 N. Cell-based experiments shown that RNAi decreased <i>mCherry</i> expression by 81.7% (<i>p</i> &lt; 0.001). This effect was decreased by 8.0% in the presence of SARS-CoV-2 N, indicating that SARS-CoV-2 N inhibits RNAi. With increasing of folic acid concentration, <i>mCherry</i> expression decreased, indicating that folic acid antagonises the regulatory effect of SARS-CoV-2 N on host RNAi. Folic acid may be an antagonist of SARS-CoV-2 N, but its effect on viruses unclear. In future, the mechanisms of action of folic acid against SARS-CoV-2 N should be studied.
DOI: 10.6084/m9.figshare.19199360
title: Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription JieZe-1 in protecting against HSV-2 infection

description: The Chinese herbal prescription JieZe-1 (JZ-1) is effective against HSV-2 (<i>Herpes simplex</i> virus type 2) infection. However, its mechanism remains unclear. To explore the mechanism of JZ-1 in protecting against HSV-2 infection. Using the methods of network pharmacology, the hub components and targets were screened and functionally enriched. We established a genital herpes (GH) mouse model and observe the disease characteristics. Then, the GH mice in different groups (10 per/group) were treated with 20 μL JZ-1 gel (2.5, 1.5, and 0.5 g/mL), acyclovir gel (0.03 g/mL), or plain carbomer gel twice a day. The symptom score, vulvar histomorphology, and virus load were measured. The critical proteins of caspase-1–dependent pyroptosis were analysed by microscopy, co-immunoprecipitation, western blotting, and ELISA. Molecular docking was also performed. Network pharmacology analysis identified 388 JZ-1 targets related to HSV-2 infection, with 36 hub targets and 21 hub components screened. The TCID<sub>50</sub> of HSV-2 was 1 × 10<sup>−7</sup>/0.1 mL. JZ-1 gel (2.5 g/mL) can effectively reduce the symptom score (81.23%), viral load (98.42%) and histopathological changes, and significantly inhibit the proteins expression of caspase-1–dependent pyroptosis in GH mice (<i>p&lt;</i> 0.05). The molecular docking test showed a good binding potency between 11 components and caspase-1 or interleukin (IL)-1β. The present study demonstrated that JZ-1 protected mice from HSV-2 infection and inhibit the caspase-1–dependent pyroptosis in GH mice. It is of significance for the second development of JZ-1 and the exploration of new drugs.
DOI: 10.6084/m9.figshare.19941215
title: Myostain is involved in ginsenoside Rb1-mediated anti-obesity

description: Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of <i>Panax ginseng</i> C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb1 regulates obesity remain to be explored. This paper intends to further explore the mechanism of Rb1 in regulating obesity. The C57BL/6 obese mice were divided into two groups: the control (CTR) and Rb1. The CTR group [intraperitoneally (ip) administered with saline] and the Rb1 group (ip administered with Rb1, 40 mg/kg/d) were treated daily for four weeks. <i>In vitro</i>, Rb1 (0, 10, 20, 40 μM) was added to differentiated C2C12 cells and Rb1 (0, 20, 40 μM) was added to 3T3-L1 cells. After 24 h, total RNA and protein from C2C12 cells and 3T3-L1 cells were used to detect myostatin (MSTN) and fibronectin type III domain-containing 5 (FNDC5) expression. Rb1 reduced the body weight and adipocyte size. Improved glucose tolerance and increased basic metabolic activity were also found in Rb1 treated mice. MSTN was downregulated in differentiated C2C12 cells, 3T3-L1 cells and adipose tissues upon Rb1 treatment. FNDC5 was increased after Rb1 treatment. However, MSTN overexpression attenuated Rb1-mediated decrease accumulation of lipid droplets in differentiated 3T3-L1 adipocytes. Rb1 may ameliorate obesity in part through the MSTN/FNDC5 signalling pathway. Our results showed that Rb1 can be used as an effective drug in the treatment of human obesity.