cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5885506
title: BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom

description: Abstract Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom. Methods: The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). The amino acid sequence of the N-terminal region was carried out by Edman degradation method. Platelet aggregation assays were performed in human platelet-rich plasma (PRP). Infrared (IR) spectroscopy was used in order to elucidate the interactions between BaltDC and platelet membrane. Results: BaltDC ran as a single protein band on SDS-PAGE and showed apparent molecular mass of 32 kDa under reducing or non-reducing conditions. The N-terminal region of the purified protein revealed the amino acid sequence IISPPVCGNELLEVGEECDCGTPENCQNECCDA, which showed identity with other snake venom metalloproteinases (SVMPs). BaltDC was devoid of proteolytic, hemorrhagic, defibrinating or coagulant activities, but it showed a specific inhibitory effect on platelet aggregation induced by ristocetin and epinephrine in PRP. IR analysis spectra strongly suggests that PO 3 2 − groups, present in BaltDC, form hydrogen bonds with the PO 2 − groups present in the non-lipid portion of the membrane platelets. Conclusions: BaltDC may be of medical interest since it was able to inhibit platelet aggregation.
DOI: 10.6084/m9.figshare.5885506.v1
title: BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom

description: Abstract Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom. Methods: The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). The amino acid sequence of the N-terminal region was carried out by Edman degradation method. Platelet aggregation assays were performed in human platelet-rich plasma (PRP). Infrared (IR) spectroscopy was used in order to elucidate the interactions between BaltDC and platelet membrane. Results: BaltDC ran as a single protein band on SDS-PAGE and showed apparent molecular mass of 32 kDa under reducing or non-reducing conditions. The N-terminal region of the purified protein revealed the amino acid sequence IISPPVCGNELLEVGEECDCGTPENCQNECCDA, which showed identity with other snake venom metalloproteinases (SVMPs). BaltDC was devoid of proteolytic, hemorrhagic, defibrinating or coagulant activities, but it showed a specific inhibitory effect on platelet aggregation induced by ristocetin and epinephrine in PRP. IR analysis spectra strongly suggests that PO 3 2 − groups, present in BaltDC, form hydrogen bonds with the PO 2 − groups present in the non-lipid portion of the membrane platelets. Conclusions: BaltDC may be of medical interest since it was able to inhibit platelet aggregation.
DOI: 10.6084/m9.figshare.5885500
title: Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

description: Abstract Background: Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros. Methods: The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (&lt;7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assayed for ACE inhibition and, in vivo, for edema potentiation. Results: Typical BPP signatures were identified in three RP-HPLC fractions. CID fragmentation presented the usual y-ion of the terminal P-P fragment as a predominant signal at m/z 213.1. De novo peptide sequencing identified one Bothrops-like BPP and one new BPP sequence. The new BPP was synthesized and showed poor inhibition over ACE, but displayed significant bradykinin-induced edema potentiation. Conclusions: So far, few BPPs are described in Viperinae, and based on the sequenced peptides, two non-canonical sequences were detected. The possible clinical role of this new peptides remains unclear.
DOI: 10.6084/m9.figshare.5885500.v1
title: Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

description: Abstract Background: Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros. Methods: The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (&lt;7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assayed for ACE inhibition and, in vivo, for edema potentiation. Results: Typical BPP signatures were identified in three RP-HPLC fractions. CID fragmentation presented the usual y-ion of the terminal P-P fragment as a predominant signal at m/z 213.1. De novo peptide sequencing identified one Bothrops-like BPP and one new BPP sequence. The new BPP was synthesized and showed poor inhibition over ACE, but displayed significant bradykinin-induced edema potentiation. Conclusions: So far, few BPPs are described in Viperinae, and based on the sequenced peptides, two non-canonical sequences were detected. The possible clinical role of this new peptides remains unclear.
DOI: 10.6084/m9.figshare.5885497
title: Towards an ethical-responsible linguistic education: reflections on discursive practices in an English language classroom

description: ABSTRACT Recent studies in Applied Linguistics (AL) have developed a dialogue with Bakhtinian theorizations and the teaching and learning of languages. Such studies agree that language is permeated by discourses that dialogue with themselves, going beyond the concept of language as a simple rigid system of structures that can be analyzed in syntactic, morphological, and phonetic aspects. Following these premises, we aim to discuss, based on the Bakhtinian concepts of subject, dialogism, ideology, centripetal/centrifugal forces, and ethical act, the discursive practices of English language students. To this end, we make use of parts of a lesson based on the video The danger of a single story, by Chimamanda Adichie, which problematizes essentialized truths about the other. Based on the discussion between the empirical material generated and the theoretical framework, we reflect on the importance of the ethical-responsible formation of students in order to answer contemporary educational demands.
DOI: 10.6084/m9.figshare.5885497.v1
title: Towards an ethical-responsible linguistic education: reflections on discursive practices in an English language classroom

description: ABSTRACT Recent studies in Applied Linguistics (AL) have developed a dialogue with Bakhtinian theorizations and the teaching and learning of languages. Such studies agree that language is permeated by discourses that dialogue with themselves, going beyond the concept of language as a simple rigid system of structures that can be analyzed in syntactic, morphological, and phonetic aspects. Following these premises, we aim to discuss, based on the Bakhtinian concepts of subject, dialogism, ideology, centripetal/centrifugal forces, and ethical act, the discursive practices of English language students. To this end, we make use of parts of a lesson based on the video The danger of a single story, by Chimamanda Adichie, which problematizes essentialized truths about the other. Based on the discussion between the empirical material generated and the theoretical framework, we reflect on the importance of the ethical-responsible formation of students in order to answer contemporary educational demands.
DOI: 10.6084/m9.figshare.5885491
title: Information ecosystems: New paradigm for Information Architecture

description: Abstract This article defends the hypothesis that information ecosystems from the Internet of Things represent a new paradigm for Information Architecture. To discuss the issue, we enunciate concepts and epistemological foundations of Information Architecture. Finally, we seek understanding information ecosystems in this new reality, with people, objects and places connected everywhere, communicating with each other, creating a potentially transformative dimension to society. Finally, we describe the sub-discipline Pervasive Information Architecture as a specialty of Information Architecture, which emerges to deal with the challenges ahead. The research is based on the Meta-Modeling Methodology and in a critical analysis of the literature of the field. As a result, we defend the need for consolidating a transdisciplinary framework of fundamentals, theories and methodologies able to solve emerging issues.
DOI: 10.6084/m9.figshare.5885491.v1
title: Information ecosystems: New paradigm for Information Architecture

description: Abstract This article defends the hypothesis that information ecosystems from the Internet of Things represent a new paradigm for Information Architecture. To discuss the issue, we enunciate concepts and epistemological foundations of Information Architecture. Finally, we seek understanding information ecosystems in this new reality, with people, objects and places connected everywhere, communicating with each other, creating a potentially transformative dimension to society. Finally, we describe the sub-discipline Pervasive Information Architecture as a specialty of Information Architecture, which emerges to deal with the challenges ahead. The research is based on the Meta-Modeling Methodology and in a critical analysis of the literature of the field. As a result, we defend the need for consolidating a transdisciplinary framework of fundamentals, theories and methodologies able to solve emerging issues.
DOI: 10.6084/m9.figshare.5885476
title: The 2010 Brazilian Oral Health Survey (SBBrasil 2010 Project): what do the coordinators propose for future surveys?

description: The objective of this paper is to analyze the suggestions made by the coordinators of the 2010 Brazilian Oral Health Survey (SBBrasil 2010 Project) for future national oral health surveys . This is a qualitative cross-sectional study. Data collection was done through an electronic semi-structured questionnaire. Participants were practitioners from the Brazilian National Health System (SUS) as well as university teachers who worked as the survey coordinators. Answers were classified into categories, according to Bardin’s content analysis. Suggestions were mainly related to changes in the sampling methodology and working team, operational aspects, financial aid, analysis and dissemination of results. We hope that this study may contribute to the discussion among the professionals of the field, seeking continued improvements of oral health surveillance in Brazil.
DOI: 10.6084/m9.figshare.5885476.v1
title: The 2010 Brazilian Oral Health Survey (SBBrasil 2010 Project): what do the coordinators propose for future surveys?

description: The objective of this paper is to analyze the suggestions made by the coordinators of the 2010 Brazilian Oral Health Survey (SBBrasil 2010 Project) for future national oral health surveys . This is a qualitative cross-sectional study. Data collection was done through an electronic semi-structured questionnaire. Participants were practitioners from the Brazilian National Health System (SUS) as well as university teachers who worked as the survey coordinators. Answers were classified into categories, according to Bardin’s content analysis. Suggestions were mainly related to changes in the sampling methodology and working team, operational aspects, financial aid, analysis and dissemination of results. We hope that this study may contribute to the discussion among the professionals of the field, seeking continued improvements of oral health surveillance in Brazil.
DOI: 10.6084/m9.figshare.5885473
title: The role of Family Health Support Center in assistance coordination of Primary Health Care: limits and possibilities

description: ABSTRACT The aim of this study was to analyze how the Family Health Support Center has contributed to Family Health Teams in the fulfillment of its assistance coordination function. A qualitative case study was performed in the city of Camaragibe, State of Pernambuco. Data collection was held from January 2015 to May 2015 by means of focal groups, document analysis, and participatory observation. Results demonstrate that the Family Health Support Center, which acts following the logic of matrix support, is able to collaborate for the reinforcement of the Primary Health assistance coordination function.
DOI: 10.6084/m9.figshare.5885473.v1
title: The role of Family Health Support Center in assistance coordination of Primary Health Care: limits and possibilities

description: ABSTRACT The aim of this study was to analyze how the Family Health Support Center has contributed to Family Health Teams in the fulfillment of its assistance coordination function. A qualitative case study was performed in the city of Camaragibe, State of Pernambuco. Data collection was held from January 2015 to May 2015 by means of focal groups, document analysis, and participatory observation. Results demonstrate that the Family Health Support Center, which acts following the logic of matrix support, is able to collaborate for the reinforcement of the Primary Health assistance coordination function.
DOI: 10.6084/m9.figshare.5885467
title: Why has a large Legionnaires’ disease outbreak been absent from public debate?

description: The Legionnaires’ disease outbreak that hit Portugal in November 2014 was the third largest worldwide and was declared a “great public health emergency”. Nonetheless, the Portuguese outbreak, despite killing 12 people and infecting 375 others did not promote extensive media coverage, nor did it make it into the political debate. We conducted a quantitative analysis of 83 news pieces on Legionella published in four national newspapers, and interviewed the journalists who covered this outbreak. The communication process was controlled by a small group of official sources and the outbreak was pushed away from news lineups due to two political scandals. The production of another news wave made the outbreak’s news wave to break prematurely.
DOI: 10.6084/m9.figshare.5885467.v1
title: Why has a large Legionnaires’ disease outbreak been absent from public debate?

description: The Legionnaires’ disease outbreak that hit Portugal in November 2014 was the third largest worldwide and was declared a “great public health emergency”. Nonetheless, the Portuguese outbreak, despite killing 12 people and infecting 375 others did not promote extensive media coverage, nor did it make it into the political debate. We conducted a quantitative analysis of 83 news pieces on Legionella published in four national newspapers, and interviewed the journalists who covered this outbreak. The communication process was controlled by a small group of official sources and the outbreak was pushed away from news lineups due to two political scandals. The production of another news wave made the outbreak’s news wave to break prematurely.
DOI: 10.6084/m9.figshare.5885464
title: The sensitivity of sunflower (Helianthus annuus L.) plants to UV-B radiation is altered by nitrogen status

description: ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama) plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control) or 8.0W m-2 (+UV-B) of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm) or high (282ppm) nitrogen as ammonium nitrate. High nitrogen increased dry matter of stem, leaves and shoot, photosynthetic pigments and photosynthesis (A) without any alteration in stomatal conductance (gs) nor transpiration (E) while it reduced the intercellular CO2 (Ci) concentration, and malondialdehyde (MDA) content. High UV-B radiation had negative effects on dry matter production, A, gs and E with the effects more marked under high nitrogen, whereas it increased Ci under high nitrogen. Activity of PG-POD was reduced by high UV-B radiation under low nitrogen but it was not changed under high nitrogen. The UV-B radiation increased the MDA content independently of nitrogen level. Results indicate that the effects of UV-B radiation on sunflower plants are dependent of nitrogen supply with high nitrogen making their physiological processes more sensitive to UV-B radiation.
DOI: 10.6084/m9.figshare.5885464.v1
title: The sensitivity of sunflower (Helianthus annuus L.) plants to UV-B radiation is altered by nitrogen status

description: ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama) plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control) or 8.0W m-2 (+UV-B) of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm) or high (282ppm) nitrogen as ammonium nitrate. High nitrogen increased dry matter of stem, leaves and shoot, photosynthetic pigments and photosynthesis (A) without any alteration in stomatal conductance (gs) nor transpiration (E) while it reduced the intercellular CO2 (Ci) concentration, and malondialdehyde (MDA) content. High UV-B radiation had negative effects on dry matter production, A, gs and E with the effects more marked under high nitrogen, whereas it increased Ci under high nitrogen. Activity of PG-POD was reduced by high UV-B radiation under low nitrogen but it was not changed under high nitrogen. The UV-B radiation increased the MDA content independently of nitrogen level. Results indicate that the effects of UV-B radiation on sunflower plants are dependent of nitrogen supply with high nitrogen making their physiological processes more sensitive to UV-B radiation.
DOI: 10.6084/m9.figshare.5885461
title: Health, care and risk: what do they mean to adolescents living in the Mare favela, Rio de Janeiro, Brazil

description: This is a qualitative study about the meanings of health, care and risk to Brazilian adolescents living in the Maré favela, in Rio de Janeiro. The analysis is oriented by reflections on the epidemiological perspective that classifies this age group as a risk group, and the media impacts on social relations, through the increase of the circulation of information on the subject. The research had as source the group discussions with adolescents aged between 14 and 16 years old. The study showed competing discourses regarding the risk culture, family traditions and the context of violence in everyday life of the participants. The study highlights the mediation of mothers, physicians working in primary care and the media on the social construction of these meanings.
DOI: 10.6084/m9.figshare.5885461.v1
title: Health, care and risk: what do they mean to adolescents living in the Mare favela, Rio de Janeiro, Brazil

description: This is a qualitative study about the meanings of health, care and risk to Brazilian adolescents living in the Maré favela, in Rio de Janeiro. The analysis is oriented by reflections on the epidemiological perspective that classifies this age group as a risk group, and the media impacts on social relations, through the increase of the circulation of information on the subject. The research had as source the group discussions with adolescents aged between 14 and 16 years old. The study showed competing discourses regarding the risk culture, family traditions and the context of violence in everyday life of the participants. The study highlights the mediation of mothers, physicians working in primary care and the media on the social construction of these meanings.
DOI: 10.6084/m9.figshare.5885455
title: Effect of warm-up intensity on horse-rider dyad’s performance in jumping

description: ABSTRACT: The aim of this study was to determine whether there is a correlation between the intensity of warm-up and scores of horse-rider dyads in jumping competitions, and which factors affect the warm-up regime. Three international competitions of 120, 130, and 135cm obstacle height, completed by 82 competitors, were studied. Warm-up intensity was measured by the time spent in the schooling area, number of practice jumping efforts, and coefficient of practice obstacle height. Out of the official final scores in the competitions, penalty points in the round and converted final placings were used as outcome measures. The data were analyzed with Spearman’s correlation, multifactorial analysis of variance, and Tukey’s test. The rider’s sex, part of the world that the riders came from, horse’s sex, age, and competitive level (obstacle height) were considered. Results showed that more jumps and higher obstacles during warm-up decrease the horse-rider dyad’s performance in jumping competition, whereas the duration of the total warm-up time does not affect the score. Female riders warm up horses longer but jump lower fences than male riders. Riders do not differentiate the warm-up regime with regard to the horse’s sex, whereas they considered the horse’s age. The warm-up intensity does not increase proportionally to the competitive level.