DOI: 10.25403/upresearchdata.29381234.v1
title: Assessment of <i>E. coli</i> reduction in artificially contaminated borehole water via Sand-Zero valent iron-Moringa Filtration"

description: The research investigates the efficacy of a novel multi-layered filtration system combining sand, zero-valent iron (ZVI), and Moringa oleifera-functionalised sand (f-sand) for improving the microbiological and physicochemical quality of borehole water used for spinach irrigation. ​ The study aims to address microbial contamination, particularly Escherichia coli, in irrigation water, soil, and spinach plants, while also evaluating the system's impact on water quality parameters. ​Key Objectives:<b>Literature Review</b>: Assess existing water filtration methods using sand, ZVI, and Moringa oleifera for microbial contamination mitigation. ​<b>Filtration System Development</b>: Construct laboratory-scale filtration systems (sand-only, sand-ZVI, and sand-ZVI-Moringa) and optimize their filtration rates and contact times. ​<b>Efficacy Testing</b>: Evaluate the ability of these systems to reduce E. coli levels in artificially inoculated borehole water, soil, and spinach plants in a greenhouse setting.<b>Physicochemical Analysis</b>: Assess the impact of filtration treatments on water quality parameters such as pH, turbidity, nitrate levels, and dissolved oxygen. ​Research Highlights:<b>Network Analysis</b>: A bibliometric study revealed a growing interest in plant-based coagulants like Moringa oleifera for water treatment, with significant advancements in functionalised sand filtration systems over the last two decades. ​<b>Filtration Trials</b>: Two trials were conducted using spinach plants irrigated with filtered water. ​ Results showed that sand-ZVI-Moringa filters were the most effective in reducing E. coli levels in water, soil, and spinach leaves compared to sand-only and sand-ZVI filters. ​<b>Physicochemical Parameters</b>: While most water quality parameters remained within acceptable limits, turbidity and nitrate levels occasionally exceeded guidelines, highlighting areas for system optimization. ​<b>Mechanisms of Action</b>: ZVI generates reactive oxygen species (ROS) that inactivate bacteria, while Moringa oleifera's cationic proteins disrupt bacterial membranes and enhance pathogen removal. ​Findings:Sand-ZVI-Moringa filtration significantly reduced E. coli levels in irrigation water and minimized its transfer to soil and spinach leaves. ​The system demonstrated potential for improving irrigation water safety, but challenges such as turbidity and nitrate removal need further optimization. ​Physicochemical parameters like pH, dissolved oxygen, and iron content were generally within permissible limits, confirming the system's stability. ​Conclusion:The study introduces a scalable, cost-effective filtration system that integrates natural and engineered materials to address microbial contamination in irrigation water. It highlights the potential of sand-ZVI-Moringa filters to enhance water quality and food safety in resource-limited agricultural settings. ​ Further research is needed to refine the system for broader adoption and regulatory compliance. ​