cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7562639
title: Increased Expression Levels of Metalloprotease, Tissue Inhibitor of Metalloprotease, Metallothionein, and p63 in Ectopic Endometrium: An Animal Experimental Study

description: Abstract Objective To characterize the patterns of cell differentiation, proliferation, and tissue invasion in eutopic and ectopic endometrium of rabbits with induced endometriotic lesions via a well- known experimental model, 4 and 8 weeks after the endometrial implantation procedure. Methods Twenty-nine female New Zealand rabbits underwent laparotomy for endometriosis induction through the resection of one uterine horn, isolation of the endometrium, and fixation of tissue segment to the pelvic peritoneum. Two groups of animals (one with 14 animals, and the other with15) were sacrificed 4 and 8 weeks after endometriosis induction. The lesion was excised along with the opposite uterine horn for endometrial gland and stroma determination. Immunohistochemical reactions were performed in eutopic and ectopic endometrial tissues for analysis of the following markers: metalloprotease (MMP-9) and tissue inhibitor of metalloprotease (TIMP-2), which are involved in the invasive capacity of the endometrial tissue; and metallothionein (MT) and p63, which are involved in cell differentiation and proliferation. Results The intensity of the immunostaining for MMP9, TIMP-2, MT, and p63 was higher in ectopic endometria than in eutopic endometria. However, when the ectopic lesions were compared at 4 and 8 weeks, no significant difference was observed, with the exception of the marker p63, which was more evident after 8 weeks of evolution of the ectopic endometrial tissue. Conclusion Ectopic endometrial lesions seem to express greater power for cell differentiation and tissue invasion, compared with eutopic endometria, demonstrating a potentially invasive, progressive, and heterogeneous presentation of endometriosis.
DOI: 10.6084/m9.figshare.7562639.v1
title: Increased Expression Levels of Metalloprotease, Tissue Inhibitor of Metalloprotease, Metallothionein, and p63 in Ectopic Endometrium: An Animal Experimental Study

description: Abstract Objective To characterize the patterns of cell differentiation, proliferation, and tissue invasion in eutopic and ectopic endometrium of rabbits with induced endometriotic lesions via a well- known experimental model, 4 and 8 weeks after the endometrial implantation procedure. Methods Twenty-nine female New Zealand rabbits underwent laparotomy for endometriosis induction through the resection of one uterine horn, isolation of the endometrium, and fixation of tissue segment to the pelvic peritoneum. Two groups of animals (one with 14 animals, and the other with15) were sacrificed 4 and 8 weeks after endometriosis induction. The lesion was excised along with the opposite uterine horn for endometrial gland and stroma determination. Immunohistochemical reactions were performed in eutopic and ectopic endometrial tissues for analysis of the following markers: metalloprotease (MMP-9) and tissue inhibitor of metalloprotease (TIMP-2), which are involved in the invasive capacity of the endometrial tissue; and metallothionein (MT) and p63, which are involved in cell differentiation and proliferation. Results The intensity of the immunostaining for MMP9, TIMP-2, MT, and p63 was higher in ectopic endometria than in eutopic endometria. However, when the ectopic lesions were compared at 4 and 8 weeks, no significant difference was observed, with the exception of the marker p63, which was more evident after 8 weeks of evolution of the ectopic endometrial tissue. Conclusion Ectopic endometrial lesions seem to express greater power for cell differentiation and tissue invasion, compared with eutopic endometria, demonstrating a potentially invasive, progressive, and heterogeneous presentation of endometriosis.
DOI: 10.6084/m9.figshare.7562630
title: Fatty acid profile in erythrocytes associated with serum cytokines in pediatric cystic fibrosis patients

description: ABSTRACT Objective To analyze erythrocyte fatty acid composition and its association with serum cytokine levels in pediatric cystic fibrosis patients. Methods A cross-sectional study was performed at a reference center in Rio de Janeiro, Brazil. We have included all pediatric patients aged 5-19 years with confirmed cystic fibrosis diagnosis. Erythrocyte fatty acid composition and serum cytokine (TNF-α, IL-1β, IL-6 and IL-8) and C-reactive protein levels were measured. The cut-off point to determine essential fatty acids deficiency was the linoleic acid concentration of &lt;21%. Results Twenty-six children (&lt;10 years old) and thirty-one adolescents were studied. Most patients were female and heterozygous for DF508 mutation and suffered from exocrine pancreatic insufficiency. Both children and adolescents had lower linoleic acid concentration (&lt;21%). TNF-α was the only pro-inflammatory marker whose levels were increased; the increase was greater in children. An association between fatty acid composition in erythrocytes and cytokines IL-1β and IL-6 was observed (p&lt;0.05). Conclusion The pediatric cystic fibrosis patients studied presented a deficiency of essential fatty acids, and an association between fatty acid profile in erythrocytes and serum pro-inflammatory cytokines was observed. These findings highlight the importance of this type of assessment that may open new possibilities for studying pathophysiology and treating cystic fibrosis patients, such as the dietary supplementation with n-3 fatty acids (eicosapentaenoic and docosahexaenoic acids). However, further longitudinal studies are needed for better clarification of the imbalance in lipid metabolism and inflammation in cystic fibrosis
DOI: 10.6084/m9.figshare.7562627
title: Pelvic floor electromyography and urine flow patterns in children with vesicoureteral reflux and lower urinary tract symptoms

description: ABSTRACT Objective: To determine the different urine flow patterns and active pelvic floor electromyography (EMG) during voiding in children with vesicoureteral reflux (VUR) as well as presenting the prevalence of lower urinary tract symptoms in these patients. Materials and Methods: We retrospectively reviewed the charts of children diagnosed with VUR after toilet training from Sep 2013 to Jan 2016. 225 anatomically and neurologically normal children were included. The reflux was diagnosed with voiding cystourethrography. The study was comprised an interview by means of a symptom questionnaire, a voiding diary, uroflowmetry with EMG and kidney and bladder ultrasounds. Urine flow patterns were classified as bell shape, staccato, interrupted, tower and plateau based on the current International Children's Continence Society guidelines. Results: Of 225 children with VUR (175 girls, 50 boys), underwent uroflowmetry + EMG, 151 (67.1%) had an abnormal urine flow pattern. An active pelvic floor EMG during voiding was confirmed in 113 (50.2%) children. The flow patterns were staccato in 76 (33.7 %), interrupted in 41 (18.2%), Plateau in 26 (11.5%), tower in 12 (5.3%) and a bell shape or normal pattern in 70 (31.5%). Urinary tract infection, enuresis and constipation respectively, were more frequent symptoms in these patients. Conclusions: Bladder/bowel dysfunction is common in patients with VUR that increases the risk of breakthrough urinary tract infections in children receiving antibiotic prophylaxis and reduces the success rate for endoscopic injection therapy. Therefore investigation of voiding dysfunction with primary assessment tools can be used prior to treating VUR.
DOI: 10.6084/m9.figshare.7562630.v1
title: Fatty acid profile in erythrocytes associated with serum cytokines in pediatric cystic fibrosis patients

description: ABSTRACT Objective To analyze erythrocyte fatty acid composition and its association with serum cytokine levels in pediatric cystic fibrosis patients. Methods A cross-sectional study was performed at a reference center in Rio de Janeiro, Brazil. We have included all pediatric patients aged 5-19 years with confirmed cystic fibrosis diagnosis. Erythrocyte fatty acid composition and serum cytokine (TNF-α, IL-1β, IL-6 and IL-8) and C-reactive protein levels were measured. The cut-off point to determine essential fatty acids deficiency was the linoleic acid concentration of &lt;21%. Results Twenty-six children (&lt;10 years old) and thirty-one adolescents were studied. Most patients were female and heterozygous for DF508 mutation and suffered from exocrine pancreatic insufficiency. Both children and adolescents had lower linoleic acid concentration (&lt;21%). TNF-α was the only pro-inflammatory marker whose levels were increased; the increase was greater in children. An association between fatty acid composition in erythrocytes and cytokines IL-1β and IL-6 was observed (p&lt;0.05). Conclusion The pediatric cystic fibrosis patients studied presented a deficiency of essential fatty acids, and an association between fatty acid profile in erythrocytes and serum pro-inflammatory cytokines was observed. These findings highlight the importance of this type of assessment that may open new possibilities for studying pathophysiology and treating cystic fibrosis patients, such as the dietary supplementation with n-3 fatty acids (eicosapentaenoic and docosahexaenoic acids). However, further longitudinal studies are needed for better clarification of the imbalance in lipid metabolism and inflammation in cystic fibrosis
DOI: 10.7284/118782
title: CTD (Conductivity, Temperature, Depth) data collected during research cruise HLY1301 using a Sea-Bird SBE-911+ instrument system onboard the platform USCGC Healy

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7562621
title: Factors associated with handgrip strength in older adults residents in Florianópolis, Brazil: EpiFloripa Aging Study

description: ABSTRACT Objective To investigate the association between sociodemographic factors, lifestyle, health conditions and low Handgrip Strength in older adults. Methods A cross-sectional study was conducted in a sample of 598 older adults. The Handgrip Strength (first quartile) was verified using a dynamometer. Independent variables included age group, schooling, living arrangement, paid work, alcohol consumption, smoking, leisure-time physical activity and transportation, disability in activities of daily living and instrumental activities of daily living, history of falls and probable cognitive impairment. Logistic regression analyses were performed (crude and adjusted). Results Age group, disability in instrumental activities of daily living and physical inactivity were associated with low Handgrip Strength for women. For men, there was no association. Conclusion Low Handgrip Strength was positively associated with age, disability in instrumental activities of daily living, and negatively associated with the practice of physical activity in women.
DOI: 10.6084/m9.figshare.7562627.v1
title: Pelvic floor electromyography and urine flow patterns in children with vesicoureteral reflux and lower urinary tract symptoms

description: ABSTRACT Objective: To determine the different urine flow patterns and active pelvic floor electromyography (EMG) during voiding in children with vesicoureteral reflux (VUR) as well as presenting the prevalence of lower urinary tract symptoms in these patients. Materials and Methods: We retrospectively reviewed the charts of children diagnosed with VUR after toilet training from Sep 2013 to Jan 2016. 225 anatomically and neurologically normal children were included. The reflux was diagnosed with voiding cystourethrography. The study was comprised an interview by means of a symptom questionnaire, a voiding diary, uroflowmetry with EMG and kidney and bladder ultrasounds. Urine flow patterns were classified as bell shape, staccato, interrupted, tower and plateau based on the current International Children's Continence Society guidelines. Results: Of 225 children with VUR (175 girls, 50 boys), underwent uroflowmetry + EMG, 151 (67.1%) had an abnormal urine flow pattern. An active pelvic floor EMG during voiding was confirmed in 113 (50.2%) children. The flow patterns were staccato in 76 (33.7 %), interrupted in 41 (18.2%), Plateau in 26 (11.5%), tower in 12 (5.3%) and a bell shape or normal pattern in 70 (31.5%). Urinary tract infection, enuresis and constipation respectively, were more frequent symptoms in these patients. Conclusions: Bladder/bowel dysfunction is common in patients with VUR that increases the risk of breakthrough urinary tract infections in children receiving antibiotic prophylaxis and reduces the success rate for endoscopic injection therapy. Therefore investigation of voiding dysfunction with primary assessment tools can be used prior to treating VUR.
DOI: 10.6084/m9.figshare.7562624
title: Digital Soil Mapping of Soil Properties in the “Mar de Morros” Environment Using Spectral Data

description: ABSTRACT Quantification of soil properties is essential for better understanding of the environment and better soil management. The conventional techniques of laboratory analysis are sometimes costly and detrimental to the environment. Thus, development of new techniques for soil analysis that do not generate residues, such as spectroscopy, is increasingly necessary as a viable way to estimate a wide range of soil properties. The objective of this study was to predict the levels of organic carbon (OC), clay, and extractable phosphorus (P), from the spectral responses of soil samples in the visible and near infrared (Vis-NIR), medium infrared (MIR), and Vis-NIR-MIR using different preprocessing methods combined with five prediction models. Soil samples were collected in Iconha, Espírito Santo State, Brazil, in the Ribeirão Inhaúma basin. A total of 184 samples were collected from 92 sites at two depths (0.00-0.10 and 0.10-0.30 m). Physical, chemical, and spectral analyses were performed according to routine soil laboratory methods. Random selection was made of 70 % of total samples for training and 30 % for validation of the models. The coefficient of determination (R2) and root mean square error (RMSE) were calculated in order to assess model performance. The standardized indexes of prediction error RPD and RPIQ were also calculated. For clay and OC, the best R2 was found in the MIR spectrum, at 0.69 and 0.65, respectively, and for P, it was 0.57 in Vis-NIR. The MSC (Multiplicative Scatter Correction), CR (Continuum removal), and SNV (Standard Normal Variate) preprocesses were most efficient for predicting clay, OC, and P, respectively, while the PLSR - Partial Least Squares Regression (OC and P) and SVM - Support Vector Machine (clay) gave the best predictions and are therefore recommended for modeling these properties in the study area. The models identified in this study can be used to discriminate soils according to a critical test value for clay, OC, and P.
DOI: 10.7284/119521
title: Meteorological Station data collected during research cruise HLY1301 using a SIO MET-System instrument system onboard the platform USCGC Healy

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7562621.v1
title: Factors associated with handgrip strength in older adults residents in Florianópolis, Brazil: EpiFloripa Aging Study

description: ABSTRACT Objective To investigate the association between sociodemographic factors, lifestyle, health conditions and low Handgrip Strength in older adults. Methods A cross-sectional study was conducted in a sample of 598 older adults. The Handgrip Strength (first quartile) was verified using a dynamometer. Independent variables included age group, schooling, living arrangement, paid work, alcohol consumption, smoking, leisure-time physical activity and transportation, disability in activities of daily living and instrumental activities of daily living, history of falls and probable cognitive impairment. Logistic regression analyses were performed (crude and adjusted). Results Age group, disability in instrumental activities of daily living and physical inactivity were associated with low Handgrip Strength for women. For men, there was no association. Conclusion Low Handgrip Strength was positively associated with age, disability in instrumental activities of daily living, and negatively associated with the practice of physical activity in women.
DOI: 10.6084/m9.figshare.7562624.v1
title: Digital Soil Mapping of Soil Properties in the “Mar de Morros” Environment Using Spectral Data

description: ABSTRACT Quantification of soil properties is essential for better understanding of the environment and better soil management. The conventional techniques of laboratory analysis are sometimes costly and detrimental to the environment. Thus, development of new techniques for soil analysis that do not generate residues, such as spectroscopy, is increasingly necessary as a viable way to estimate a wide range of soil properties. The objective of this study was to predict the levels of organic carbon (OC), clay, and extractable phosphorus (P), from the spectral responses of soil samples in the visible and near infrared (Vis-NIR), medium infrared (MIR), and Vis-NIR-MIR using different preprocessing methods combined with five prediction models. Soil samples were collected in Iconha, Espírito Santo State, Brazil, in the Ribeirão Inhaúma basin. A total of 184 samples were collected from 92 sites at two depths (0.00-0.10 and 0.10-0.30 m). Physical, chemical, and spectral analyses were performed according to routine soil laboratory methods. Random selection was made of 70 % of total samples for training and 30 % for validation of the models. The coefficient of determination (R2) and root mean square error (RMSE) were calculated in order to assess model performance. The standardized indexes of prediction error RPD and RPIQ were also calculated. For clay and OC, the best R2 was found in the MIR spectrum, at 0.69 and 0.65, respectively, and for P, it was 0.57 in Vis-NIR. The MSC (Multiplicative Scatter Correction), CR (Continuum removal), and SNV (Standard Normal Variate) preprocesses were most efficient for predicting clay, OC, and P, respectively, while the PLSR - Partial Least Squares Regression (OC and P) and SVM - Support Vector Machine (clay) gave the best predictions and are therefore recommended for modeling these properties in the study area. The models identified in this study can be used to discriminate soils according to a critical test value for clay, OC, and P.
DOI: 10.6084/m9.figshare.7562609
title: Comparative Study of the Use of HPA Lanolin and Breast Milk for Treating Pain Associated with Nipple Trauma

description: Abstract Objective To compare two different treatments—the use of highly purified anhydrous (HPA) lanolin and expressed breast milk—for women with pain and nipple trauma during the breastfeeding process. Method A total of 180 puerperal women were randomly assigned to 2 groups: one was treated with HPA lanolin and the other with their own expressed breast milk. All of the participants received the same breastfeeding technique instructions and therapeutic care standard. Three assessments were performed: at the time of inclusion in the study (after randomization); after 48 hours; and after 7 days. At each interval, data was collected in relation to pain and trauma. A numerical/verbal category scale was used for the pain variable, and the nipple trauma score for the trauma variable. The results were subjected to statistical analysis using the chi-squared test, the Fisher exact test, the student t-test, and the Kolmogorov-Smirnov test. Generalized estimating equations were calculated using the STATA 12 statistical software package (StataCorp LLC, College Station, TX, USA) and IBM SPSS Statistics for Windows, Version 20.0 (IBM Corp, Armonk, NY, USA). Results There was pain improvement from the second to the third assessment in the group that used HPA lanolin, while the pain remained unchanged between these two periods (p&lt; 0.001) in the breast milk group. In terms of trauma, improvement was identified in its extension and depth from the first to the third assessment, and it was higher in the HPA lanolin group than in the breast milk group (p= 0.025). Conclusion The treatment of pain and nipple trauma with HPA lanolin achieved better results than the one with breast milk, based on a 7-day treatment period.
DOI: 10.6084/m9.figshare.7562612
title: Ideal Cardiovascular Health and Job Strain: A Cross-Sectional Study from the Amazon Basin

description: Abstract Background: Ideal Cardiovascular (CV) Health is characterized by four ideal lifestyle parameters and absence of cardiovascular risk factors. The prevalence of ideal CV health in the Amazon Basin and the influence of job strain on CV health in this setting are uncertain. Objective: To evaluate the prevalence of ideal CV health and its relationship with job strain in a secluded area from a developing country. Methods: Job strain was evaluated in 478 employees from an university in the Amazon Basin by a questionnaire that classified participants as passive, active, low or high strain, according to the demand-control model. CV health was evaluated using the American Heart Association 7 health factors (diet, physical activity, body mass index (BMI), smoking, hypertension, diabetes and hypercholesterolemia). Participants were classified as having ideal, intermediate or poor CV health. The level of significance was set at p &lt; 0.05. Results: The mean age was 44.3 ± 12 years, 65% were men, and 35% were faculty. No participant fulfilled the criteria for ideal CV health. Intermediate CV health was found in 44 (9%) and poor in 434 (91%) individuals. Considering low strain as a reference group, individuals classified as high strain, active and passive had a non-significant (p &gt; 0.05) increase in the chances of having poor CV health. When adjusting for possible confounders, high job strain was associated with poor BMI (&gt; 30 kg/m2), (OR 2.11, 95%CI 1.06-4.22; p = 0.034) and poor diet (OR 2.31, 95% CI 1.29-4.13; p = 0.005). Conclusion: Job strain was not associated with cardiovascular health, but high job strain was related to obesity and poor diet. Given the high prevalence of poor CV health and lack of participants with ideal CV health, policies focusing on health education and lifestyle interventions are paramount to this population.