DOI: 10.5061/dryad.dv41ns1wf
title: Data from: Variability of the atmospheric PM10 microbiome in three climatic regions of France

description: Air pollution is a major public-health concern and it is recognized that
 particulate matter causes damage to human health through oxidative-stress,
 being responsible for several million premature deaths worldwide each
 year. Recent findings showed that, airborne microorganisms/spores can
 modulate aerosol toxicity by altering the oxidative potential of PM10.
 Primary Biogenic Organic Aerosols (PBOA) appears to be produced by only
 few genera of microorganisms, emitted by surrounding vegetation in the
 case of a regionally-homogeneous field site. This study presents the first
 comprehensive description of the structure and main sources of airborne
 microbial communities associated with the temporal trends in PM10 SC
 concentrations at 3 French sites, under different climates. By combining
 sugar chemistry and DNA Metabarcoding approaches, we intended to identify
 PM10-associated microbial communities and their main sources at three
 climatically different sampling-sites in France during summer 2018. This
 study accounted also for the interannual variability of the structure of
 summer airborne microbial community associated with PM10-SC concentrations
 during a consecutive 2-year survey at one site. Our results showed that
 the temporal evolutions of SC in PM10 in the three sites are associated
 with the abundance of only few specific airborne fungal and bacterial
 taxa. These taxa differ significantly between the 3 climatic regions
 studied. The structure of microbial communities associated with PM10 SC
 concentrations during a consecutive 2-year survey remained stable in the
 rural area. The atmospheric concentration levels of PM10 SC-species vary
 significantly between the 3 studied sites, but with no clear difference
 according to site typology (rural vs urban), suggesting that SC emissions
 are more related to regional climatic characteristics. The overall
 microbial beta diversity in PM10 samples is significantly different from
 that of the main vegetation around the studied urban sites. This indicates
 that airborne microorganisms at such urban sites do not originate only
 from the immediate surrounding vegetation, which is contrasting with
 observations at the scale of a regionally homogeneous rural site made in
 2017. These results improve our understanding of the spatial behavior of
 tracers of PBOA emission sources, which need to be better characterized to
 further implement this important OM-mass fraction into CTM models.
description: obitools commands used in the analysis of data
 This file contains the obitools commands used to manipulate
 sequence reads, filter sequences, and assign taxonomy
 obitools commands.txt
description: Database of MOTUs and sequences Bacterial
 and fungal community compositions were surveyed using respectively the
 Bact02 and Fung02 primer pairs. This files contain different type of
 samples for two consecutive years: letter 'A' corresponds to
 aerosols sampled in summer 2017, while 'P' corresponds to those
 collected in summer 2018. 'S' indicates soil samples collected
 during summer 2017. Letters 'F' and 'X' corresponds to
 leaf samples. P1 to P20 correspond to aerosols
 collected at Marseille site; P21 to P36 corresponds those collected at OPE
 sit; P37 to P60 indicate aerosols collected at Grenoble site. 
 Letters 'FM', 'X1 to X3' correspond to leaf
 samples collected at Marseille site; Letters 'FG' correspond
 to leaf samples collected at Grenoble site.