cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20038475
title: Analysis of Fusarium ear rot and fumonisin contamination in testcrosses of a maize biparental population

description: Abstract Fusarium ear rot (FER) negatively affects maize production worldwide and poses a serious threat to human health for producing mycotoxins. The objectives of our study were to assess the factors affecting FER and fumonisin contamination in testcrosses of a maize population. In the trials, seeds of 191 testcrosses of IBMSyn4, 11 checks and 2 replications of parental lines (a total of 216 hybrids) were sown in three environments, in 2014 and 2015. FER disease intensity (DI), fumonisin contamination (FUMc) and number of ears with signs of European corn borer (ECB) attack were measured. Strong phenotypic and weak genetic correlations between DI, FUMc and ECB indicate randomness in the interaction of those traits. We detected three QTLs (chr.1, 2 and 6) for DI (LOD scores 3.77-5.06). The QTL on chr.2, confirmed across the environments, can serve as a guideline in breeding for FER resistance.
DOI: 10.6084/m9.figshare.20038475.v1
title: Analysis of Fusarium ear rot and fumonisin contamination in testcrosses of a maize biparental population

description: Abstract Fusarium ear rot (FER) negatively affects maize production worldwide and poses a serious threat to human health for producing mycotoxins. The objectives of our study were to assess the factors affecting FER and fumonisin contamination in testcrosses of a maize population. In the trials, seeds of 191 testcrosses of IBMSyn4, 11 checks and 2 replications of parental lines (a total of 216 hybrids) were sown in three environments, in 2014 and 2015. FER disease intensity (DI), fumonisin contamination (FUMc) and number of ears with signs of European corn borer (ECB) attack were measured. Strong phenotypic and weak genetic correlations between DI, FUMc and ECB indicate randomness in the interaction of those traits. We detected three QTLs (chr.1, 2 and 6) for DI (LOD scores 3.77-5.06). The QTL on chr.2, confirmed across the environments, can serve as a guideline in breeding for FER resistance.
DOI: 10.6084/m9.figshare.20038477.v1
title: SNP markers associated with soybean partial resistance to Phytophthora sojae

description: Abstract Phytophthora root and stem rot is one of the most aggressive diseases in soybean crop. The use of resistant cultivars is the main strategy to reduce losses caused by the pathogen. This study aims to identify SNP markers associated with genes or QTLs that provide soybean with partial resistance to Phytophthora sojae. A total of 169 soybean cultivars were inoculated with Phytophthora sojae and genotyped with 3,807 SNP markers. Genome-wide association analysis was carried out via multiple linear models, followed by multiple linear regression and linkage disequilibrium analysis. Four QTLs associated with the characteristic were identified: two on chromosome 3 and two on chromosome 15. The regions containing these QTLs contain genes already annotated as providers of resistance to pathogens, in plants. The use of those markers in the selection of resistant plants can increase the efficiency of breeding programs in the development of soybean varieties resistant to P. sojae.
DOI: 10.6084/m9.figshare.20038477
title: SNP markers associated with soybean partial resistance to Phytophthora sojae

description: Abstract Phytophthora root and stem rot is one of the most aggressive diseases in soybean crop. The use of resistant cultivars is the main strategy to reduce losses caused by the pathogen. This study aims to identify SNP markers associated with genes or QTLs that provide soybean with partial resistance to Phytophthora sojae. A total of 169 soybean cultivars were inoculated with Phytophthora sojae and genotyped with 3,807 SNP markers. Genome-wide association analysis was carried out via multiple linear models, followed by multiple linear regression and linkage disequilibrium analysis. Four QTLs associated with the characteristic were identified: two on chromosome 3 and two on chromosome 15. The regions containing these QTLs contain genes already annotated as providers of resistance to pathogens, in plants. The use of those markers in the selection of resistant plants can increase the efficiency of breeding programs in the development of soybean varieties resistant to P. sojae.
DOI: 10.6084/m9.figshare.20038478
title: Selection of Eucalyptus grandis families across contrasting environmental conditions

description: Abstract The objective was to select productive and stable E. grandis families across contrasting sites in Brazil and Uruguay. Survival and growth of 130 open pollinated families were evaluated three years after planting. Survival ranged from 28 to 89% and mean annual increment from 20.5 to 48.4 m3ha- y-1. Low productivity in one site was attributed to unusually low rainfall and in the other, because of Cylindrocladium leaf disease. Heritability among families and the correlation family by environment were intermediate. Genetic pairwise correlations ranged from 0.03 to 0.81 across sites. The lowest genetic correlation between sites was observed where Cylindrocladium leaf disease occurs. Selection of top families across sites allowed selecting the best ones in each site. However, the effect of genotype-environment interactions was observed where climatic conditions are not adequate to the E. grandis, favoring the occurrence of Cylindrocladium disease.
DOI: 10.6084/m9.figshare.20038478.v1
title: Selection of Eucalyptus grandis families across contrasting environmental conditions

description: Abstract The objective was to select productive and stable E. grandis families across contrasting sites in Brazil and Uruguay. Survival and growth of 130 open pollinated families were evaluated three years after planting. Survival ranged from 28 to 89% and mean annual increment from 20.5 to 48.4 m3ha- y-1. Low productivity in one site was attributed to unusually low rainfall and in the other, because of Cylindrocladium leaf disease. Heritability among families and the correlation family by environment were intermediate. Genetic pairwise correlations ranged from 0.03 to 0.81 across sites. The lowest genetic correlation between sites was observed where Cylindrocladium leaf disease occurs. Selection of top families across sites allowed selecting the best ones in each site. However, the effect of genotype-environment interactions was observed where climatic conditions are not adequate to the E. grandis, favoring the occurrence of Cylindrocladium disease.
DOI: 10.6084/m9.figshare.20038480
title: Development of a mulberry core collection originated in China to enhance germplasm conservation

description: Abstract China, origin of mulberry, has rich genetic resources usually. High expense, limited space and wavering environment of usually conservation in vivo poses dangerous situation for mulberry. The concept of core collection could takes priority for conservation of mulberry. In this study, 560 accessions were used with 40 morphological descriptors and stratified sampling strategies for a core collection. The core collection consisted of 28 accessions, accounting for 5% of the whole collection. The core collection included seven accessions belonging to Morus alba, one accession belonging to M. alba var. macrophylla, four accessions belonging to M. atropurprea, one accession belonging to M. nigra, three accessions belonging to M. australis, seven accessions belonging to M. multicaulis, two accessions belonging to M. wittorum and three accessions belonging to M. bombycis. The quality of core collection exceeded the evaluation criteria and could be a prioritized collection for high efficient and long-term conservation for mulberry.
DOI: 10.6084/m9.figshare.20038480.v1
title: Development of a mulberry core collection originated in China to enhance germplasm conservation

description: Abstract China, origin of mulberry, has rich genetic resources usually. High expense, limited space and wavering environment of usually conservation in vivo poses dangerous situation for mulberry. The concept of core collection could takes priority for conservation of mulberry. In this study, 560 accessions were used with 40 morphological descriptors and stratified sampling strategies for a core collection. The core collection consisted of 28 accessions, accounting for 5% of the whole collection. The core collection included seven accessions belonging to Morus alba, one accession belonging to M. alba var. macrophylla, four accessions belonging to M. atropurprea, one accession belonging to M. nigra, three accessions belonging to M. australis, seven accessions belonging to M. multicaulis, two accessions belonging to M. wittorum and three accessions belonging to M. bombycis. The quality of core collection exceeded the evaluation criteria and could be a prioritized collection for high efficient and long-term conservation for mulberry.
DOI: 10.6084/m9.figshare.20038486
title: Mapping of QTL for aluminum tolerance in tropical maize

description: Abstract The objectives of this study were to map genomic regions associated with QTL for aluminum (Al) tolerance in maize and determine the phenotypic effects of tolerance loci. QTL analysis for Al tolerance was carried out in a population of F2:3 progenies resulting from a cross between the contrasting lines LT 99-4 and LS 04-2. SSR (Simple Sequence Repeat) loci and AFLP (Amplified Fragment Length Polymorphism) were used to construct the linkage map and to detect QTL mapped by composite interval mapping analysis. Nine tolerance QTL among eight linkage groups (chromosomes 2, 4, 5, 6, 7, 8, 9, and 10) were mapped, which explained 70.3% of the phenotypic variance. The results confirmed three major QTL (bins 6.00, 8.05, and 10.01) that are described in the literature for Al tolerance, which accounted for most of the phenotypic variance (40.3%).
DOI: 10.6084/m9.figshare.20038486.v1
title: Mapping of QTL for aluminum tolerance in tropical maize

description: Abstract The objectives of this study were to map genomic regions associated with QTL for aluminum (Al) tolerance in maize and determine the phenotypic effects of tolerance loci. QTL analysis for Al tolerance was carried out in a population of F2:3 progenies resulting from a cross between the contrasting lines LT 99-4 and LS 04-2. SSR (Simple Sequence Repeat) loci and AFLP (Amplified Fragment Length Polymorphism) were used to construct the linkage map and to detect QTL mapped by composite interval mapping analysis. Nine tolerance QTL among eight linkage groups (chromosomes 2, 4, 5, 6, 7, 8, 9, and 10) were mapped, which explained 70.3% of the phenotypic variance. The results confirmed three major QTL (bins 6.00, 8.05, and 10.01) that are described in the literature for Al tolerance, which accounted for most of the phenotypic variance (40.3%).
DOI: 10.6084/m9.figshare.20038487
title: Effect of a mutation in Raffinose Synthase 2 (GmRS2 ) on soybean quality traits

description: Abstract The presence of stachyose and raffinose is considered an antinutritional factor for humans and monogastric animals, leading to limitations on soybean consumption as a protein source. In the present study, the effect of a mutation in the raffinose synthase 2 gene was measured on soybean quality traits. We used an F2 population with 168 soybean individuals developed by crossing four soybean lines and evaluated oil, protein, sucrose, raffinose, stachyose and fatty acid contents and their relationships. The mutation explained 69.61%, 51.81% and 31.96% of stachyose, raffinose and sucrose variation, respectively, and we were able to produce soybean with average stachyose of 0.18%. The low coefficients of determination for protein and oil indicate that the mutation can be used to increase sucrose and reduce raffinose and stachyose content without major changes in oil and protein.
DOI: 10.6084/m9.figshare.20038487.v1
title: Effect of a mutation in Raffinose Synthase 2 (GmRS2 ) on soybean quality traits

description: Abstract The presence of stachyose and raffinose is considered an antinutritional factor for humans and monogastric animals, leading to limitations on soybean consumption as a protein source. In the present study, the effect of a mutation in the raffinose synthase 2 gene was measured on soybean quality traits. We used an F2 population with 168 soybean individuals developed by crossing four soybean lines and evaluated oil, protein, sucrose, raffinose, stachyose and fatty acid contents and their relationships. The mutation explained 69.61%, 51.81% and 31.96% of stachyose, raffinose and sucrose variation, respectively, and we were able to produce soybean with average stachyose of 0.18%. The low coefficients of determination for protein and oil indicate that the mutation can be used to increase sucrose and reduce raffinose and stachyose content without major changes in oil and protein.
DOI: 10.6084/m9.figshare.20038489
title: Recurrent selection in common bean aiming at resistance to white mold in a greenhouse

description: Abstract The objectives of this study were to estimate the genetic progress of mass selection for white mold resistance in common bean, evaluated in a greenhouse in cycle XII of recurrent selection (900 S0 plants), and compare it with field selection in previous cycles. In addition, progress was compared with microsatellite marker-assisted selection (MAS) among S0:1 progenies. The 79 most resistant S0:1 were evaluated under field conditions using a 9×9 simple lattice design; the 21 best S0:2 and those selected from cycles IX, X, and XI were evaluated in a 6×6 triple lattice. Genetic progress was 4.25% per cycle, and 12.17% between cycles XI and XII, showing higher selection efficiency in the greenhouse. The phenotypic gain and gain from assisted selection among the S0:1 progenies were 5.08 and 1.57%, respectively, and the low value of MAS was due to only two markers (BM189 and BMD20) explaining the resistance.
DOI: 10.6084/m9.figshare.20038489.v1
title: Recurrent selection in common bean aiming at resistance to white mold in a greenhouse

description: Abstract The objectives of this study were to estimate the genetic progress of mass selection for white mold resistance in common bean, evaluated in a greenhouse in cycle XII of recurrent selection (900 S0 plants), and compare it with field selection in previous cycles. In addition, progress was compared with microsatellite marker-assisted selection (MAS) among S0:1 progenies. The 79 most resistant S0:1 were evaluated under field conditions using a 9×9 simple lattice design; the 21 best S0:2 and those selected from cycles IX, X, and XI were evaluated in a 6×6 triple lattice. Genetic progress was 4.25% per cycle, and 12.17% between cycles XI and XII, showing higher selection efficiency in the greenhouse. The phenotypic gain and gain from assisted selection among the S0:1 progenies were 5.08 and 1.57%, respectively, and the low value of MAS was due to only two markers (BM189 and BMD20) explaining the resistance.
DOI: 10.6084/m9.figshare.20038490
title: Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

description: Abstract To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F1), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.
DOI: 10.6084/m9.figshare.20038490.v1
title: Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

description: Abstract To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F1), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.
DOI: 10.6084/m9.figshare.20038493.v1
title: ‘UENF 506-11’: a new maize cultivar for the North and Northwest of Rio de Janeiro State

description: Abstract The intervarietal hybrid ‘UENF 506-11’ was derived from full-sib maize families in the 11th cycle of reciprocal recurrent selection. It produces a grain yield of 7.57 tons ha-1, and is recommended for cultivation in the North and Northwest regions of the Rio de Janeiro State.
DOI: 10.6084/m9.figshare.20038493
title: ‘UENF 506-11’: a new maize cultivar for the North and Northwest of Rio de Janeiro State

description: Abstract The intervarietal hybrid ‘UENF 506-11’ was derived from full-sib maize families in the 11th cycle of reciprocal recurrent selection. It produces a grain yield of 7.57 tons ha-1, and is recommended for cultivation in the North and Northwest regions of the Rio de Janeiro State.
DOI: 10.6084/m9.figshare.20038495
title: Cytogenetic characterization of Angelonia integerrima Sprengel, a native species with ornamental potential

description: Abstract Angelonia integerrima Sprengel is a native species of the state of Mato Grosso do Sul and the Southern region (Paraná, Santa Catarina and Rio Grande do Sul) of Brazil, with features such as an unusual appearance and color of the flowers, indicating an ornamental use. To optimize the use of this species and to fill in gaps regarding its cytogenetic characterization, this study determined the chromosome number, meiotic index and pollen viability of plant individuals of four A. integerrima populations. All plant individuals of the four populations had 2n = 20 chromosomes. Still, the meiotic index of most analyzed plant individuals exceeded 90%, while pollen viability of all plant individuals was higher than 80%. These data suggest considerable cytological stability of the analyzed A. integerrima plant individuals, which may favor the selection of future genotypes for commercial purposes or their use in conservation and breeding programs of the species.
DOI: 10.6084/m9.figshare.20038495.v1
title: Cytogenetic characterization of Angelonia integerrima Sprengel, a native species with ornamental potential

description: Abstract Angelonia integerrima Sprengel is a native species of the state of Mato Grosso do Sul and the Southern region (Paraná, Santa Catarina and Rio Grande do Sul) of Brazil, with features such as an unusual appearance and color of the flowers, indicating an ornamental use. To optimize the use of this species and to fill in gaps regarding its cytogenetic characterization, this study determined the chromosome number, meiotic index and pollen viability of plant individuals of four A. integerrima populations. All plant individuals of the four populations had 2n = 20 chromosomes. Still, the meiotic index of most analyzed plant individuals exceeded 90%, while pollen viability of all plant individuals was higher than 80%. These data suggest considerable cytological stability of the analyzed A. integerrima plant individuals, which may favor the selection of future genotypes for commercial purposes or their use in conservation and breeding programs of the species.
DOI: 10.6084/m9.figshare.9276593
title: Inheritance of leaf color in papaya

description: Abstract Physiological disturbances are one of the major bottlenecks to the expansion of papaya crops due to the negative influence on fruit quality. Studies on genotypes of light-green color have become essential to the development of strategies of tolerance to the physiological disorder skin freckle. Understanding the inheritance of qualitative traits is crucial to selection and prediction of the behavior of segregating generations. Thus, this study aimed to determine the inheritance of the qualitative trait leaf color in segregating generations of crosses between the dark-green cultivar BSA and the light-green cultivar GPC. Inheritance was determined based on the Mendelian genetics laws, by evaluating the phenotypic proportions in the analysis of generations P1 (BSA), P2 (GPC), F1, F2, BC1, BC2, BC2r, and F2:3. The inheritance of light-green leaves from the crossing between BSA and GPG is due to double recessive epistasis.
DOI: 10.6084/m9.figshare.9276593.v1
title: Inheritance of leaf color in papaya

description: Abstract Physiological disturbances are one of the major bottlenecks to the expansion of papaya crops due to the negative influence on fruit quality. Studies on genotypes of light-green color have become essential to the development of strategies of tolerance to the physiological disorder skin freckle. Understanding the inheritance of qualitative traits is crucial to selection and prediction of the behavior of segregating generations. Thus, this study aimed to determine the inheritance of the qualitative trait leaf color in segregating generations of crosses between the dark-green cultivar BSA and the light-green cultivar GPC. Inheritance was determined based on the Mendelian genetics laws, by evaluating the phenotypic proportions in the analysis of generations P1 (BSA), P2 (GPC), F1, F2, BC1, BC2, BC2r, and F2:3. The inheritance of light-green leaves from the crossing between BSA and GPG is due to double recessive epistasis.
DOI: 10.6084/m9.figshare.9276281
title: How to shorten a plant breeding program? A case study with ornamental peppers

description: Abstract Plant breeding of ornamental peppers (Capsicum spp.) can be supported by biotechnological tools. The objective of this study was to evaluate the efficiency of an in vitro culture of immature zygotic embryos (IZE) to reduce the breeding cycle of ornamental pepper (C. annuum) in comparison to the conventional system. Three ornamental pepper genotypes were used: UFPB 001, UFPB 004, and UFPB 099. Embryos at 30 days after selfing were inoculated in MS ½-strength culture medium, and at the same time, seeds were placed to germinate in a commercial substrate. Approximately 215 days are required from selfing until fruit ripening in the conventional system, whereas the IZE system requires an average of 153 days, a decrease of approximately 30% per selection cycle, corresponding to 496 days considering 8 selfing cycles. A decrease in time, labor, and inputs makes the IZE system a suitable tool for shortening the breeding program of ornamentals peppers.