cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/6n54c-2rz79
title: Agrobacterium tumefaciens C58

description: Agro C58
description: <strong>Tilt Series Date:</strong> 2013-10-23</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Induction for T-pilus with 200uM AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-31/rawdata/</p>c58_2_berk_home_195k0002.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-31/rawdata/c58_2_berk_home_195k0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_2_berk_home_195k0002_full.rec,  Reconstruction (Pixel Size 0.0 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-31/3dimage_49028/c58_2_berk_home_195k0002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rm44z-hcg74
title: TRIM5-21R 2D lattice

description: TRIM5-21R 2D lattice prepared by Barbie Ganser-Pornillos and Viswanathan Chandrasekaran
description: <strong>Tilt Series Date:</strong> 2012-11-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Viswanathan Chandrasekarangrids purified the protein, Barbie Ganser-Pornillos assembled protein into 2D lattice. The sample was was plunge frozen and imaged at Caltech by Cora Woodward and Kelly Dryden</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-20-3/rawdata/</p>12nov20_TRIM5_21R_grid2_AT3BT1b_0003.mrc,  Tilt Series (Pixel Size 0.45 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-20-3/rawdata/12nov20_TRIM5_21R_grid2_AT3BT1b_0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        12nov20_TRIM5_21R_grid2_AT3BT1b_0003_full.rec,  Reconstruction (Pixel Size 1.8 nm), 530.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-20-3/3dimage_23766/12nov20_TRIM5_21R_grid2_AT3BT1b_0003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w41rp-p4h08
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-13</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-27/rawdata/</p>12mar13_c121_1_Cos74ADN_AT1bt1_0021.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-27/rawdata/12mar13_c121_1_Cos74ADN_AT1bt1_0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar13_c121_1_Cos74ADN_AT1bt1_0021_full.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-27/3dimage_17827/12mar13_c121_1_Cos74ADN_AT1bt1_0021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ftawm-k7531
title: M-MuLV 43D infected MEFs

description: M-MuLV 43D wildtype
description: <strong>Tilt Series Date:</strong> 2014-03-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> pipeline raptor</p>
<strong>Collaborators and Roles:</strong> M-MuLV infected cells provided by Hung Fan (UC Irvine)</p>
<strong>Sample Preparation:</strong> Whole MEFs infected with M-MuLV 43D were cultured on Au grids, blotted manually and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-03-29-10/rawdata/</p>14mar29_C182-1_MLV43D_3T3_AT1BT2_0001.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-03-29-10/rawdata/14mar29_C182-1_MLV43D_3T3_AT1BT2_0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        14mar29_C182-1_MLV43D_3T3_AT1BT2_0001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-03-29-10/3dimage_40575/14mar29_C182-1_MLV43D_3T3_AT1BT2_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mvsnt-ad564
title: HeLa cells

description: Raw data files of HeLa cells
description: <strong>Tilt Series Date:</strong> 2014-11-13</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> cells and siRNA obtained from Doug Mackay/ Cathrine Ullman</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA NUP153+ Thymidine 2mM, 14 h post thymidine release</p>
  rra2014-11-13-59_slicer10590.jpg: Upper- microtubule .  lower- connection rra2014-11-13-59_slicer10599.jpg: Microtubules criss cross
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-13-59/rawdata/</p>midbody70012.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-13-59/rawdata/midbody70012.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody70012_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-13-59/3dimage_48112/midbody70012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6hfbq-xn211
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-10/3dimage_69379/</p>12may29_C135_1_MEFnomitoDNA_AT2BT20001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-10/3dimage_69379/12may29_C135_1_MEFnomitoDNA_AT2BT20001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may29_C135_1_MEFnomitoDNA_AT2BT20001.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-10/rawdata/12may29_C135_1_MEFnomitoDNA_AT2BT20001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d77kq-a3n66
title: E.coli   "strain 2750"

description: E.coli 2570 tsr-G213A I214A K215Ahigh kinase-on output, but receptor can still respond to attractnt stimuli
description: <strong>Tilt Series Date:</strong> 2012-04-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB at 30 degrees, lysed with Penicillin for one hour</p>
  aba2012-04-04-12_slicer9780.jpg: Escherichia coli .  periplasmic vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-04-04-12/rawdata/</p>UU27500006.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-04-04-12/rawdata/UU27500006.mrc"
        > <i class="download icon"></i> Download </a></p>

        UU27500006_part128_82.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-04-04-12/3dimage_18195/UU27500006_part128_82.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ccb7j-k5m92
title: HUVECs infx pNLGFP

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 19000.
description: <strong>Tilt Series Date:</strong> 2013-07-05</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Sample Preparation:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-29/rawdata/</p>13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0001.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-29/rawdata/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0001_full.rec,  Reconstruction (Pixel Size 2.24 nm), 602.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-29/3dimage_28060/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.e3pntm
title: Occurrence Download

description: A dataset containing 109 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "GadmGid is CHN.28_1",
 "TaxonKey is Salsola tragus"
 ]
}

 The dataset includes 109 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002424-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/h5xkp-rs231
title: Agro pJW58

description: wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter.  Kanamycin-resistant (150 to 300 ug/mL)
description: <strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-16/rawdata/</p>Agro_pJW58_30001.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-16/rawdata/Agro_pJW58_30001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Agro_pJW58_30001_part121_47.rec,  Reconstruction (Pixel Size 1.6 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-16/3dimage_39701/Agro_pJW58_30001_part121_47.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yv8vf-9gg13
title: Agrobacterium tumefaciens

description: VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB + antibiotics</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-16/rawdata/</p>vg3101_30007.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-16/rawdata/vg3101_30007.mrc"
        > <i class="download icon"></i> Download </a></p>

        vg3101_30007_part111_45.rec,  Reconstruction (Pixel Size 0.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-16/3dimage_48943/vg3101_30007_part111_45.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/27bc1-en717
title: E. coli Minicells M259Q

description: E.coli Minicells, receptor tsr M259Q on plasmid
description: <strong>Tilt Series Date:</strong> 2013-12-18</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Minicell prep, lysed</p>
  aba2013-12-18-39_slicer9523.jpg: Escherichia coli .  vesicle aba2013-12-18-39_slicer9524.jpg: Escherichia coli .  extracellular vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-18-39/rawdata/</p>colimini0019.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-18-39/rawdata/colimini0019.mrc"
        > <i class="download icon"></i> Download </a></p>

        colimini0019_part121_2.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-18-39/3dimage_36329/colimini0019_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ra68p-2eg40
title: Vibrio harveyi

description: wild type Vibrio harveyi BB120
description: <strong>Tilt Series Date:</strong> 2013-12-24</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio harveyi</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Kirsten Jung</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in ab medium</p>
  aba2013-12-24-13_slicer3630.jpg: Vibrio harveyi: polar filament bundle aba2013-12-24-13_slicer7137.jpg: Pilli aba2013-12-24-13_slicer7139.jpg: Filament bundle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-24-13/rawdata/</p>BB120VibrioHWT0013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-24-13/rawdata/BB120VibrioHWT0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        BB120VibrioHWT0013SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-24-13/3dimage_46425/BB120VibrioHWT0013SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ed71v-7pm68
title: MJ007 sacculi

description: sacculi purification of sporulating cells
description: <strong>Tilt Series Date:</strong> 2012-03-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kit Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> See E Tocheva Mol Micro 2013</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-36/rawdata/</p>mj0025.mrc,  Tilt Series (Pixel Size 1.9 nm), 925.0 MB  
        
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        mj0025_part112_2.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
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