cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14275007.v1
title: Individual selection of the first backcross generation of passion fruit potentially resistant to the fruit woodiness disease

description: Abstract The REML/BLUP procedure has been successfully used for genetic progress through individual selection of high-yielding passion fruit genotypes resistant to the Cowpea aphid-borne mosaic virus. This study was thus developed to estimate genetic parameters and predict the gain obtained from individual selection of genotypes in a population derived from backcrosses in passion fruit. The experiment was set up as a randomized block design with four replicates, involving five full-sib families (genotypes from the first backcross). Variance components and the genetic values were estimated for eight agronomic traits via the REML/BLUP procedure. For all traits, genotypic variance between the genotypes from the first backcross showed little contribution to the phenotypic variance. The low heritability estimates obtained for the traits are overcome via individual BLUP estimates. Therefore, it was possible to obtain considerable gains with individual selection for the variables fruit length, average fruit weight, and pulp weight (19.50 to 14.04%; 22.93 to 17.97%; and 10.08 to 7.95%, respectively). For the traits showing lower gains, it is possible to obtain gains indirectly by selecting genotypes for correlated traits. Because this population derives from the first backcross generation, agronomic traits still must be recovered.
DOI: 10.6084/m9.figshare.14275014
title: Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments

description: Abstract In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.
DOI: 10.6084/m9.figshare.14275014.v1
title: Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments

description: Abstract In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.
DOI: 10.6084/m9.figshare.14275016
title: Cymbopogon flexuosus essential oil as an additive improves growth, biochemical and physiological responses and survival against Aeromonas hydrophila infection in Nile tilapia

description: Abstract The objective of the present study was to evaluate growth, biochemical, hematological and intestinal enzymes responses and survival of Nile tilapia juveniles fed a diet containing the essential oil of lemongrass Cymbopogum flexuosus (EOCF) and infected by Aeromonas hydrophila. Five diets were evaluated (in quadruplicate) with increasing levels of EOCF (0.0 - control; 0.25; 0.50; 1.0 and 2.0 mL kg diet-1). On day 45, eight fish per treatment were sampled and blood, liver and intestine samples were taken. Others eight fish per treatment were infected with A. hydrophila followed by a 15-day period of observation. Citral is the main constituent of EOCF. The inclusion of 2.0 mL EOCF kg diet-1 increased specific growth rate and survival after A. hydrophila infection and decreased feed conversion ratio of Nile tilapia. In general, higher concentrations of EOCF in the diet reduced plasma glucose and triglycerides levels, and increased plasma amino acids, alanine aminotransferase (ALT) and hepatic ALT levels, hematological parameters, and the activity of intestinal enzymes. It was concluded that the inclusion of 2.0 mL EOCF kg diet-1 improved growth performance, biochemical and physiological responses and decreased mortality of Nile tilapia after A. hydrophila infection.
DOI: 10.6084/m9.figshare.14275020
title: Weight and body yield of selectively bred tambaqui (Colossoma macropomum) farmed in different environments

description: Abstract The objective of this study was to evaluate the weight and body yield of two families of selectively bred tambaqui farmed in different environments. Two families (FA and FB) were reared in the municipalities (environments) of Santo Antônio de Leverger (MT) and Campo Grande (MS) for 431 days. Pre-bleeding weight, body yield, and the interaction effect between families and environments on these traits were investigated. No interaction effect between the evaluated families and environments was detected on the evaluated traits. Pre-bleeding weight did not differ significantly between the families in MT (FA: 2,421.7g; FB: 2,478.0g) or in MS (FA: 1,138.7g; FB: 1,389.8g), but the fish from MT had a higher (P&lt;0.05) pre-bleeding weight than those farmed in MS. The visceral fat yield (considering the two environments) was higher (P&lt;0.05) in FB family (3.8%) than in FA family (3.3%).Fish from MS showed higher (P&lt;0.05) offal yield (10.6%) and visceral fat yield (3.9%) but a lower clean-trunk yield (70.6%) than the tambaqui farmed in MT (offal: 7.7%; visceral fat: 3.1%; and clean trunk: 72.6%). In conclusion, the MT environment provides higher pre-bleeding weight and clean-trunk yield and lower offal and visceral-fat yields than the MS environment.
DOI: 10.6084/m9.figshare.14275020.v1
title: Weight and body yield of selectively bred tambaqui (Colossoma macropomum) farmed in different environments

description: Abstract The objective of this study was to evaluate the weight and body yield of two families of selectively bred tambaqui farmed in different environments. Two families (FA and FB) were reared in the municipalities (environments) of Santo Antônio de Leverger (MT) and Campo Grande (MS) for 431 days. Pre-bleeding weight, body yield, and the interaction effect between families and environments on these traits were investigated. No interaction effect between the evaluated families and environments was detected on the evaluated traits. Pre-bleeding weight did not differ significantly between the families in MT (FA: 2,421.7g; FB: 2,478.0g) or in MS (FA: 1,138.7g; FB: 1,389.8g), but the fish from MT had a higher (P&lt;0.05) pre-bleeding weight than those farmed in MS. The visceral fat yield (considering the two environments) was higher (P&lt;0.05) in FB family (3.8%) than in FA family (3.3%).Fish from MS showed higher (P&lt;0.05) offal yield (10.6%) and visceral fat yield (3.9%) but a lower clean-trunk yield (70.6%) than the tambaqui farmed in MT (offal: 7.7%; visceral fat: 3.1%; and clean trunk: 72.6%). In conclusion, the MT environment provides higher pre-bleeding weight and clean-trunk yield and lower offal and visceral-fat yields than the MS environment.
DOI: 10.6084/m9.figshare.14275016.v1
title: Cymbopogon flexuosus essential oil as an additive improves growth, biochemical and physiological responses and survival against Aeromonas hydrophila infection in Nile tilapia

description: Abstract The objective of the present study was to evaluate growth, biochemical, hematological and intestinal enzymes responses and survival of Nile tilapia juveniles fed a diet containing the essential oil of lemongrass Cymbopogum flexuosus (EOCF) and infected by Aeromonas hydrophila. Five diets were evaluated (in quadruplicate) with increasing levels of EOCF (0.0 - control; 0.25; 0.50; 1.0 and 2.0 mL kg diet-1). On day 45, eight fish per treatment were sampled and blood, liver and intestine samples were taken. Others eight fish per treatment were infected with A. hydrophila followed by a 15-day period of observation. Citral is the main constituent of EOCF. The inclusion of 2.0 mL EOCF kg diet-1 increased specific growth rate and survival after A. hydrophila infection and decreased feed conversion ratio of Nile tilapia. In general, higher concentrations of EOCF in the diet reduced plasma glucose and triglycerides levels, and increased plasma amino acids, alanine aminotransferase (ALT) and hepatic ALT levels, hematological parameters, and the activity of intestinal enzymes. It was concluded that the inclusion of 2.0 mL EOCF kg diet-1 improved growth performance, biochemical and physiological responses and decreased mortality of Nile tilapia after A. hydrophila infection.
DOI: 10.6084/m9.figshare.14275017
title: Compatibility in pollen-pistil interaction of interspecific crossings with Passiflora spp.

description: Abstract Intraspecies or interspecies crossings transfer relevant alleles between plants. However, some interspecies crossings involving Passiflora species impede ovule fertilization and the viable development of seeds. Thus, the purpose of this study was to verify the viability of interspecific crossings and monitor pollen tube development. The experiment had six species of Passiflora in the reciprocal crossings. Histochemical tests aimed to evaluate the percentage of intraspecies or interspecies crossings that resulted in fruit development and pollen tube development. Ovule fertilization and fruit development occurred in determined directions of crossings when controlling the female or male genitor, but only one case of reciprocal crossing had success. In crossings with no fruit development, histological analysis showed that some callus developed in the stigma and style, confirming unilateral and interspecies incompatibility in the genus Passiflora to some species and some directions of crossings.
DOI: 10.6084/m9.figshare.14275017.v1
title: Compatibility in pollen-pistil interaction of interspecific crossings with Passiflora spp.

description: Abstract Intraspecies or interspecies crossings transfer relevant alleles between plants. However, some interspecies crossings involving Passiflora species impede ovule fertilization and the viable development of seeds. Thus, the purpose of this study was to verify the viability of interspecific crossings and monitor pollen tube development. The experiment had six species of Passiflora in the reciprocal crossings. Histochemical tests aimed to evaluate the percentage of intraspecies or interspecies crossings that resulted in fruit development and pollen tube development. Ovule fertilization and fruit development occurred in determined directions of crossings when controlling the female or male genitor, but only one case of reciprocal crossing had success. In crossings with no fruit development, histological analysis showed that some callus developed in the stigma and style, confirming unilateral and interspecies incompatibility in the genus Passiflora to some species and some directions of crossings.
DOI: 10.6084/m9.figshare.14275022
title: Effect of salinity on embryo-larval development of yellow clam Mesodesma mactroides (Reeve, 1854) in laboratory

description: Abstract Abstract: This study assessed the effect of salinity on embryonic development, larval growth and survival of the yellow clam Mesodesma mactroides in laboratory. Embryos and larvae of M. mactroides were submitted and maintained at four different salinities: 20, 25, 30 and 35 ppt, to determine optimal conditions for the species. Through descriptive analysis, the results showed that the embryos tolerate salinities between 25 - 35 ppt, presenting fast metamorphoses at salinities 30 and 35 ppt, during experimental period of 27 hours. The same tolerance pattern was observed in larval stage (25 - 35 ppt), showing a better development in salinity of 35 ppt. This result is verified in biometric analyzes of height and length of the shells and survival rate, with higher averages in treatments with salinity 35 ppt. The experimental period of this stage lasted 27 days, when the larvae were able to settle. These results indicate that embryos and larvae of M. mactroides tolerate salinities between (25-35 ppt), with the best growth and survival on high salinities being recommended to better yields in laboratory.
DOI: 10.6084/m9.figshare.14275023.v1
title: Diversity and phenology of epigeal Coleoptera assemblages in lettuce and tomato crops in Northern Buenos Aires province, Argentina

description: Abstract Epigeic coleopteran species are linked by complex trophic webs and implicated in several ecosystem services for agriculture. Although there are reports of ground-dwelling Coleopterans inhabiting main extensive agroecosystems, their assemblages in horticultural crops in Argentina have been little explored. We examined the community structure of epigeal Coleoptera assemblages of Curculionidae, Carabidae, Staphylinidae and Coccinellidae species, in lettuce and tomato crops located in Northern Buenos Aires province, over a 3-year sampling period (2010 to 2013) by using pit-fall traps. Crop species and cycles, and phytosanitary measures (conventional and organic farming), were main factors considered as influencing the insect species composition, abundance and seasonal occurrence in the assemblages. Seventy coleopteran species were identified. The curculionids Ceutorhynchini sp. and Phyrdenus muriceus (Germar), the carabid Scarithes anthracinus Dejean, the coccinelid Eriopis connexa (Germar) and the staphylinids Aleochara sp. and Aleochara obscurus Bernhauer are the most common species in lettuce and tomato crops in the region studied. Especially the carabids, staphylinids and E. connexa are known act as natural enemies of several pests in the agroecosystems studied. Coleopteran assemblages found in lettuce differed from those associated to tomato crops, being the cropping season the main factor that influenced the community structure.
DOI: 10.6084/m9.figshare.14275022.v1
title: Effect of salinity on embryo-larval development of yellow clam Mesodesma mactroides (Reeve, 1854) in laboratory

description: Abstract Abstract: This study assessed the effect of salinity on embryonic development, larval growth and survival of the yellow clam Mesodesma mactroides in laboratory. Embryos and larvae of M. mactroides were submitted and maintained at four different salinities: 20, 25, 30 and 35 ppt, to determine optimal conditions for the species. Through descriptive analysis, the results showed that the embryos tolerate salinities between 25 - 35 ppt, presenting fast metamorphoses at salinities 30 and 35 ppt, during experimental period of 27 hours. The same tolerance pattern was observed in larval stage (25 - 35 ppt), showing a better development in salinity of 35 ppt. This result is verified in biometric analyzes of height and length of the shells and survival rate, with higher averages in treatments with salinity 35 ppt. The experimental period of this stage lasted 27 days, when the larvae were able to settle. These results indicate that embryos and larvae of M. mactroides tolerate salinities between (25-35 ppt), with the best growth and survival on high salinities being recommended to better yields in laboratory.
DOI: 10.6084/m9.figshare.14275031
title: Nutrient digestibility, nitrogen excretion, and milk production of mid-lactation Jersey × Friesian cows fed diets containing different proportions of rumen-undegradable protein

description: Abstract The present study was planned to test the hypothesis that feeding lactating dairy cattle with varying levels of rumen-undegradable protein (RUP) can enhance protein utilization, milk production, milk protein, and nitrogen (N) excretion. Forty mid-lactating crossbred (Jersey × Friesian) cattle were randomly divided into four groups. Four treatment diets were formulated to contain 30%, 40%, 50%, and 60% RUP of crude protein. Dry matter (DM) and crude protein intakes were significantly reduced with increasing dietary RUP levels. Crude protein digestibility increased linearly with incremental increases in dietary RUP levels. Cattle fed 60% RUP showed a linear decrease in N intake compared to that in the other groups. A linear decrease in urinary N and linear increases in net N, milk N, and N-use efficiency were observed with increasing dietary RUP levels. Actual milk, energy-corrected milk, and 4% fat-corrected milk yields (kg/day) increased linearly with an increasing degradability of protein. However, milk protein, solids not fat and total solids, as well as the yields of protein, fat, and lactose, showed significant increases with increased RUP supplementation. Collectively, the results indicate that formulating dairy cow diets to contain 60% RUP results in better lactating performance and N-use efficiency and lower N excretion.
DOI: 10.6084/m9.figshare.14275028
title: Production costs in the log processing of Eucalyptus spp. wood

description: Abstract The objective of this study was to evaluate the production costs of processing logs from Eucalyptus grandis Hill ex Maiden and Eucalyptus saligna Sm, using two sawing pattern in a medium-sized sawmill. Alternating tangential sawing pattern was used for logs with a mean diameter of 33.0 cm and the radial sawing pattern for logs with a mean diameter of 47.0 cm. Based on the data obtained in the different sawing pattern, the fixed, variable and total costs were calculated and, subsequently, the average value for each of these costs. For the alternating tangential sawing pattern, the fixed cost represented 19.32% and the variable cost 80.68%. For the radial sawing pattern, the fixed cost represented 18.92% and the variable cost 81.08%. The radial sawing pattern presented lower average production costs, with a difference of 22.89 R$/m³, however there is no significant difference between the sawing pattern.
DOI: 10.6084/m9.figshare.14275031.v1
title: Nutrient digestibility, nitrogen excretion, and milk production of mid-lactation Jersey × Friesian cows fed diets containing different proportions of rumen-undegradable protein

description: Abstract The present study was planned to test the hypothesis that feeding lactating dairy cattle with varying levels of rumen-undegradable protein (RUP) can enhance protein utilization, milk production, milk protein, and nitrogen (N) excretion. Forty mid-lactating crossbred (Jersey × Friesian) cattle were randomly divided into four groups. Four treatment diets were formulated to contain 30%, 40%, 50%, and 60% RUP of crude protein. Dry matter (DM) and crude protein intakes were significantly reduced with increasing dietary RUP levels. Crude protein digestibility increased linearly with incremental increases in dietary RUP levels. Cattle fed 60% RUP showed a linear decrease in N intake compared to that in the other groups. A linear decrease in urinary N and linear increases in net N, milk N, and N-use efficiency were observed with increasing dietary RUP levels. Actual milk, energy-corrected milk, and 4% fat-corrected milk yields (kg/day) increased linearly with an increasing degradability of protein. However, milk protein, solids not fat and total solids, as well as the yields of protein, fat, and lactose, showed significant increases with increased RUP supplementation. Collectively, the results indicate that formulating dairy cow diets to contain 60% RUP results in better lactating performance and N-use efficiency and lower N excretion.
DOI: 10.6084/m9.figshare.14275032
title: Protein, Phytate and Minerals in Grains of Commercial Cowpea Genotypes

description: Abstract The objective of this study was to investigate and characterize cowpea (Vigna unguiculata) genotypes for total grain protein content, storage protein fractions (globulin, albumin, prolamin, basic and acid glutelins), and phytate and minerals contents. Eighteen cowpea genotypes were selected. Total grain protein content varied from 21.4% to 29.2%, for BRS Marataoã and Paulistinha genotypes, respectively. The variation in the concentration of each protein fraction was significant (P&lt;0.05) only for glutelins (basic and acid). The genotypes studied exhibited great similarity in the PAGE electrophoretic profile of the grain protein fractions and also in the mineral content. BRS Paraguaçu genotype exhibited higher Zn content than thegenotypes that have been previously recommended for this characteristic. The lowest phytate grain content was observed in four of the 18 genotypes studied, which also exhibited high protein contents. Although the results did not converge to the selection of a few genotypes, some specific differences were detected that which may be further explored. Considering total grain protein, mineral and phytate contents, the genotype Paulistinha revealed a better balance unveiling high grain total protein content, low grain phytate content and more homogeneous mineral composition.
DOI: 10.6084/m9.figshare.14275023
title: Diversity and phenology of epigeal Coleoptera assemblages in lettuce and tomato crops in Northern Buenos Aires province, Argentina

description: Abstract Epigeic coleopteran species are linked by complex trophic webs and implicated in several ecosystem services for agriculture. Although there are reports of ground-dwelling Coleopterans inhabiting main extensive agroecosystems, their assemblages in horticultural crops in Argentina have been little explored. We examined the community structure of epigeal Coleoptera assemblages of Curculionidae, Carabidae, Staphylinidae and Coccinellidae species, in lettuce and tomato crops located in Northern Buenos Aires province, over a 3-year sampling period (2010 to 2013) by using pit-fall traps. Crop species and cycles, and phytosanitary measures (conventional and organic farming), were main factors considered as influencing the insect species composition, abundance and seasonal occurrence in the assemblages. Seventy coleopteran species were identified. The curculionids Ceutorhynchini sp. and Phyrdenus muriceus (Germar), the carabid Scarithes anthracinus Dejean, the coccinelid Eriopis connexa (Germar) and the staphylinids Aleochara sp. and Aleochara obscurus Bernhauer are the most common species in lettuce and tomato crops in the region studied. Especially the carabids, staphylinids and E. connexa are known act as natural enemies of several pests in the agroecosystems studied. Coleopteran assemblages found in lettuce differed from those associated to tomato crops, being the cropping season the main factor that influenced the community structure.