cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6208088.v1
title: The Gene Ontology Differs in Bursa of Fabricius Between Two Breeds of Ducks Post Hatching by Enriching the Differentially Expressed Genes

description: ABSTRACT The bursa of Fabricius (BF) is the central humoral immune organ unique to birds. The present study investigated the possible difference on a molecular level between two duck breeds. The digital gene expression profiling (DGE) technology was used to enrich the differentially expressed genes (DEGs) in BF between the Jianchang and Nonghua-P strains of ducks. DGE data identified 195 DEGs in the bursa. Gene Ontology (GO) analysis suggested that DEGs were mainly enriched in the metabolic pathways and ribosome components. Pathways analysis identified the spliceosome, RNA transport, RNA degradation process, Jak-STAT signaling pathway, TNF signaling pathway and B cell receptor signaling pathway. The results indicated that the main difference in the BF between the two duck strains was in the capabilities of protein formation and B cell development. These data have revealed the main divergence in the BF on a molecular level between genetically different duck breeds and may help to perform molecular breeding programs in poultry in the future.
DOI: 10.6084/m9.figshare.6208085
title: Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil

description: ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities.
DOI: 10.6084/m9.figshare.6208085.v1
title: Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil

description: ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities.
DOI: 10.6084/m9.figshare.6208082
title: IN VITRO PHYTOREMEDIATION OF PERSISTENT ORGANIC POLLUTANTS BY Helianthus annuus L. PLANTS

description: Plant model systems are needed to properly conduct basic laboratory studies prior to field applications of phytoremediation. In vitro plant cultures are a useful tool for such research. This study focuses on the removal and/or degradation of 24 persistent organic pollutants under in vitro conditions by Helianthus annuus L (sunflower). The main purpose of exploiting this plant for phytoremediation process is due to its strong adaptability to adverse environments conditions such as resistance to pests, disease, and others. The study of bioremediation effects of all chemical molecules under in vitro conditions showed promising results. Sixteen out of twenty-four compounds evaluated reached up to 87% for remediation. The highest accumulation of pollutants was observed in the roots, showing that these results are consistent with the current literature. Through the study, it was observed effective absorption of POPs with logKow ranging from 4.50 to 6.91. Sunflower phytoremediation process efficiently detected heptachlor, aldrin, heptachlor epoxide, trans-chlordane, chlordane, dieldrin, DDE, DDT, methoxychlor, mirex and decachlorobiphenyl.
DOI: 10.6084/m9.figshare.6208079
title: Inclusion of Moringa Leaf Powder (Moringa oleifera) in Fodder for Feeding Japanese Quail (Coturnix coturnix japonica)

description: ABSTRACT This research evaluated the nutritional, nutraceutical, antimicrobial, as well as the growing promoter effect of Moringa oleifera (MOR) leaves flour in fodders for fattening Japanese quails. The antimicrobial activity was measured using the method of Kirby-Bauer. A completely random design with 4x2 factorial arrangement was used, fodders included 0, 7, 14, and 21% of MOR, with and without Virginiamycin (100 ppm), during 35 d of fattening, 480 one-day old unsexed quails were used, each treatment had 5 replicates with 12 quails/cage. MOR inhibited the growth of bacteria gram (+) and gram (-). The inclusion of MOR in the period from 1 to 14 d inhibited the weight gain (p&lt;0.001), increased feed conversion (p&lt;0.001), without affecting the feed intake; however, in the period of 15 to 35 d MOR did not affect weight gain and the feed intake; the hematological and biochemical profile were within the normal range for quails. The inclusion of MOR decreased (p≤0.001) cholesterol and triglycerides concentrations. Levels of aspartate aminotransferase (AST), alanine transferase (ALT), and creatinine decreased (p≤0.001) when the amount of substitution of MOR was 21%. The carcass weight and its yield with MOR up to 14% were similar (p&lt;0.001). The results of this experiment showed that flour from leaves of Moringa oleifera is a viable alternative to be included up to 14% in commercial diets of birds offering an option for AGP replacement without compromising the health of the animal and therefore its productivity.
DOI: 10.6084/m9.figshare.6208079.v1
title: Inclusion of Moringa Leaf Powder (Moringa oleifera) in Fodder for Feeding Japanese Quail (Coturnix coturnix japonica)

description: ABSTRACT This research evaluated the nutritional, nutraceutical, antimicrobial, as well as the growing promoter effect of Moringa oleifera (MOR) leaves flour in fodders for fattening Japanese quails. The antimicrobial activity was measured using the method of Kirby-Bauer. A completely random design with 4x2 factorial arrangement was used, fodders included 0, 7, 14, and 21% of MOR, with and without Virginiamycin (100 ppm), during 35 d of fattening, 480 one-day old unsexed quails were used, each treatment had 5 replicates with 12 quails/cage. MOR inhibited the growth of bacteria gram (+) and gram (-). The inclusion of MOR in the period from 1 to 14 d inhibited the weight gain (p&lt;0.001), increased feed conversion (p&lt;0.001), without affecting the feed intake; however, in the period of 15 to 35 d MOR did not affect weight gain and the feed intake; the hematological and biochemical profile were within the normal range for quails. The inclusion of MOR decreased (p≤0.001) cholesterol and triglycerides concentrations. Levels of aspartate aminotransferase (AST), alanine transferase (ALT), and creatinine decreased (p≤0.001) when the amount of substitution of MOR was 21%. The carcass weight and its yield with MOR up to 14% were similar (p&lt;0.001). The results of this experiment showed that flour from leaves of Moringa oleifera is a viable alternative to be included up to 14% in commercial diets of birds offering an option for AGP replacement without compromising the health of the animal and therefore its productivity.
DOI: 10.6084/m9.figshare.6208076
title: Numerical analysis of RC plane structures: a concentrated nonlinear effect approach

description: Abstract The present work aims to study the nonlinear behavior of reinforced concrete structures via Refined Plastic Hinge Method (RPHM). Pseudo-springs are used at the finite element ends, where the gradual loss of stiffness is determined by the combination of the normal force and bending moment (NM) in the cross section. The limiting of the uncracked, elastic and plastic regimes is done in the NM diagram. The concrete cracking is explicitly simulated with two approaches to calculate the effective moment of inertia of the cross section. The displacement-based formulation is referenced to the co-rotational system and coupled with continuation strategies to allow to overcome the possible critical points in the equilibrium paths. For validation of the numerical simulations, the results found with the proposed formulation are confronted with experimental and numerical data present in literature.
DOI: 10.6084/m9.figshare.6208076.v1
title: Numerical analysis of RC plane structures: a concentrated nonlinear effect approach

description: Abstract The present work aims to study the nonlinear behavior of reinforced concrete structures via Refined Plastic Hinge Method (RPHM). Pseudo-springs are used at the finite element ends, where the gradual loss of stiffness is determined by the combination of the normal force and bending moment (NM) in the cross section. The limiting of the uncracked, elastic and plastic regimes is done in the NM diagram. The concrete cracking is explicitly simulated with two approaches to calculate the effective moment of inertia of the cross section. The displacement-based formulation is referenced to the co-rotational system and coupled with continuation strategies to allow to overcome the possible critical points in the equilibrium paths. For validation of the numerical simulations, the results found with the proposed formulation are confronted with experimental and numerical data present in literature.
DOI: 10.6084/m9.figshare.6208073
title: Performance of the Octopus vulgaris octopus fed on mussel (Perna perna) as monodiet

description: ABSTRACT The octopus Octopus cf. vulgaris is a potential species to diversify aquaculture. Due to absence of balanced commercial diet, growth of the O. cf. vulgaris is based on natural diet with local and low-cost inputs. In Brazil, studies on experimental octopus ongrowing are recent and there is little available data. We evaluated the performance, survival and food consumption of O. vulgaris fed on mussel Perna perna for 20 days. Six octopuses with initial weight of 415±12.73g (mean±standard deviation) were divided into two groups (n=3 octopuses/group) according to the diet: MC Group (frozen mussels) and MV Group (live mussels). The Weight Gain of octopuses was 273.33±94.52g and 340.00±26.46g; the Absolute Growth Rate was 13.67±4.73 and 17.00±1.32g.dia-1 and the Specific Growth Rate of 2.95±0.58 and 2.64±0.37%.day-1 to MC and MV groups, respectively. There was no significant difference in performance between groups and the survival rate was 100%. Octopuses well accepted both diets and despite the amount of frozen mussels (129±31) was higher than in live mussels (100±19), there was no significant difference regarding the consumption between groups. Our results demonstrate that the mussel Perna perna can be used frozen or live as monodiet in O. cf. vulgaris ongrowing.
DOI: 10.6084/m9.figshare.6208073.v1
title: Performance of the Octopus vulgaris octopus fed on mussel (Perna perna) as monodiet

description: ABSTRACT The octopus Octopus cf. vulgaris is a potential species to diversify aquaculture. Due to absence of balanced commercial diet, growth of the O. cf. vulgaris is based on natural diet with local and low-cost inputs. In Brazil, studies on experimental octopus ongrowing are recent and there is little available data. We evaluated the performance, survival and food consumption of O. vulgaris fed on mussel Perna perna for 20 days. Six octopuses with initial weight of 415±12.73g (mean±standard deviation) were divided into two groups (n=3 octopuses/group) according to the diet: MC Group (frozen mussels) and MV Group (live mussels). The Weight Gain of octopuses was 273.33±94.52g and 340.00±26.46g; the Absolute Growth Rate was 13.67±4.73 and 17.00±1.32g.dia-1 and the Specific Growth Rate of 2.95±0.58 and 2.64±0.37%.day-1 to MC and MV groups, respectively. There was no significant difference in performance between groups and the survival rate was 100%. Octopuses well accepted both diets and despite the amount of frozen mussels (129±31) was higher than in live mussels (100±19), there was no significant difference regarding the consumption between groups. Our results demonstrate that the mussel Perna perna can be used frozen or live as monodiet in O. cf. vulgaris ongrowing.
DOI: 10.6084/m9.figshare.6208070
title: An interface element for numerical analysis of flat plate/shell elements with deformable connection

description: Abstract The use of numerical methods such as the finite elements method for solving structural analysis problems is becoming ever more efficient. An interface element capable of associating flat plate/shell element in three different combinations has been developed. In a structural analysis, when only the flat plate/shell’s finite elements are used in the discretization, some problems concerning dimensional variation of the transversal section, as well as overlapping of areas can occur. . The aforementioned problems can be solved by the developed interface elements that can also simulate a possible deformable connection that is existent in the association of materials with different characteristics, such as the composite steel-concrete elements. One of the applications of the finite elements developed is for the numerical simulation of composite steel-concrete elements, such as the composite beam formed by a reinforced concrete slab attached to a steel beam using a deformable connection. In this case, the concrete slab and the steel beam are discretized by the flat plate/shell element, and the deformable connection done by the interface elements. In the validation of the implemented elements, we used numerical and experimental results found in the literature, and analytical solutions considering the classical plate theory.
DOI: 10.6084/m9.figshare.6208067
title: Seismic Performance of Post-Northridge Welded Connections

description: Abstract Post-Northridge welded connections are widely used in engineering projects as lateral-force-resisting systems. In this study, we aim to assess the cyclic performance of the post-Northridge welded connections to compare the connections with each other. For this purpose, we focus on Welded Unreinforced Flange – Welded Web (WUF-W), Welded Flange Plate (WFP) and Reduced Beam Section (RBS) connections. ANSYS Finite Element Analysis (FEA) software is used to do this investigation and evaluate the adequacy of the numerical analysis through an experimental specimen from the literature. Welded connections are designed according to ANSI 358-10 and FEMA-350 using the same material properties, beam spans, and steel profiles so on to make comparison possible. Material and geometry nonlinearities are adequately considered and also hexahedral solid elements are used in three-dimensional FEA. Based on the analyses subjected to cyclic loading, the performance of the connections are compared in terms of failure modes, plastic hinge locations, hysteretic curves, energy dissipation capacities, hysteretic equivalent viscous damping ratio and initial rotational stiffness values. The results showed that using WFP provides high stiffness in a connection while using RBS provides high safety in a connection.
DOI: 10.6084/m9.figshare.6208070.v1
title: An interface element for numerical analysis of flat plate/shell elements with deformable connection

description: Abstract The use of numerical methods such as the finite elements method for solving structural analysis problems is becoming ever more efficient. An interface element capable of associating flat plate/shell element in three different combinations has been developed. In a structural analysis, when only the flat plate/shell’s finite elements are used in the discretization, some problems concerning dimensional variation of the transversal section, as well as overlapping of areas can occur. . The aforementioned problems can be solved by the developed interface elements that can also simulate a possible deformable connection that is existent in the association of materials with different characteristics, such as the composite steel-concrete elements. One of the applications of the finite elements developed is for the numerical simulation of composite steel-concrete elements, such as the composite beam formed by a reinforced concrete slab attached to a steel beam using a deformable connection. In this case, the concrete slab and the steel beam are discretized by the flat plate/shell element, and the deformable connection done by the interface elements. In the validation of the implemented elements, we used numerical and experimental results found in the literature, and analytical solutions considering the classical plate theory.
DOI: 10.6084/m9.figshare.6208067.v1
title: Seismic Performance of Post-Northridge Welded Connections

description: Abstract Post-Northridge welded connections are widely used in engineering projects as lateral-force-resisting systems. In this study, we aim to assess the cyclic performance of the post-Northridge welded connections to compare the connections with each other. For this purpose, we focus on Welded Unreinforced Flange – Welded Web (WUF-W), Welded Flange Plate (WFP) and Reduced Beam Section (RBS) connections. ANSYS Finite Element Analysis (FEA) software is used to do this investigation and evaluate the adequacy of the numerical analysis through an experimental specimen from the literature. Welded connections are designed according to ANSI 358-10 and FEMA-350 using the same material properties, beam spans, and steel profiles so on to make comparison possible. Material and geometry nonlinearities are adequately considered and also hexahedral solid elements are used in three-dimensional FEA. Based on the analyses subjected to cyclic loading, the performance of the connections are compared in terms of failure modes, plastic hinge locations, hysteretic curves, energy dissipation capacities, hysteretic equivalent viscous damping ratio and initial rotational stiffness values. The results showed that using WFP provides high stiffness in a connection while using RBS provides high safety in a connection.
DOI: 10.6084/m9.figshare.6208064
title: Modeling the creep behavior of GRFP truss structures with Positional Finite Element Method

description: Abstract This paper presents the development of a formulation, based on Positional Finite Element Method, to describe the viscoelastic mechanical behavior of space trusses. The numerical method used was chosen due to its efficiency in the applications concerning nonlinear numerical analyses. The formulation describes the positional variation over time under constant stress state (creep). The objective is to provide a way to quantify the creep behavior for space truss structures and thus contribute to the encouragement of GFRP usage in such structural components. Time-dependent behavior of such materials is one the most important factors for their use in design of structures, demanding studies about the deformations expected within the operational life of the structural systems. To perform this study, the proposed methodology considers a standard solid rheological model to describe stress-strain time-dependent law. This model is implemented in the formulation for quantify the total strain energy. The effects of the model parameters in the mechanical response of the structure with accentuated geometric nonlinearity were presented. In this analysis, it was possible to identify the influence of the elastic and the viscous moduli on the creep response. Model calibration was performed using test data obtained from literature and a GFRP transmission line tower cross-arm was simulated to predict the evolution of displacements under real operational loads. From the results, it was possible to observe a fast evolution of displacements due to the creep effect in the first 7,500 h. This increase was close to 0.6% in relation to the displacement obtained in the elastic behavior. The presented methodology provided a simple and efficient way to quantify the creep phenomenon in viscoelastic GFRP composites truss structures, as can be seen in the developed analyses.
DOI: 10.6084/m9.figshare.6208064.v1
title: Modeling the creep behavior of GRFP truss structures with Positional Finite Element Method

description: Abstract This paper presents the development of a formulation, based on Positional Finite Element Method, to describe the viscoelastic mechanical behavior of space trusses. The numerical method used was chosen due to its efficiency in the applications concerning nonlinear numerical analyses. The formulation describes the positional variation over time under constant stress state (creep). The objective is to provide a way to quantify the creep behavior for space truss structures and thus contribute to the encouragement of GFRP usage in such structural components. Time-dependent behavior of such materials is one the most important factors for their use in design of structures, demanding studies about the deformations expected within the operational life of the structural systems. To perform this study, the proposed methodology considers a standard solid rheological model to describe stress-strain time-dependent law. This model is implemented in the formulation for quantify the total strain energy. The effects of the model parameters in the mechanical response of the structure with accentuated geometric nonlinearity were presented. In this analysis, it was possible to identify the influence of the elastic and the viscous moduli on the creep response. Model calibration was performed using test data obtained from literature and a GFRP transmission line tower cross-arm was simulated to predict the evolution of displacements under real operational loads. From the results, it was possible to observe a fast evolution of displacements due to the creep effect in the first 7,500 h. This increase was close to 0.6% in relation to the displacement obtained in the elastic behavior. The presented methodology provided a simple and efficient way to quantify the creep phenomenon in viscoelastic GFRP composites truss structures, as can be seen in the developed analyses.