DOI: 10.5061/dryad.6q573n63h
title: Data from: Pharmaceutical pollution alters the cost of bacterial infection and its relationship to pathogen load

description: The relationship between pathogen proliferation and the cost of infection
 experienced by a host drives the ecology and evolution of host-pathogen
 dynamics. While environmental factors can shape this relationship, there
 is currently limited knowledge on the consequences of emerging
 contaminants, such as pharmaceutical pollutants, for the commonly observed
 trade-off between a pathogen’s growth within the host and the damage it
 causes, termed its virulence. Here, we investigated how exposure to
 fluoxetine (Prozac), a commonly detected psychoactive pollutant, could
 alter this key relationship using the water flea Daphnia magna and its
 bacterial pathogen Pasteuria ramosa as a model system. Across a variety of
 fluoxetine concentrations, we found that fluoxetine shaped the damage a
 pathogen caused, such as the reduction in fecundity or intrinsic growth
 experienced by infected individuals, but with minimal change in average
 pathogen spore loads. Instead, fluoxetine modified the relationship
 between the degree of pathogen proliferation and its virulence, with both
 the strength of this trade-off and the component of host fitness most
 affected varying by fluoxetine concentration and host genotype. Our study
 underscores the potential for pharmaceutical pollution to modify the
 virulence of an invading pathogen, as well as the fundamental trade-off
 between host and pathogen fitness, even at the trace amounts increasingly
 found in natural waterways.
description: # Data for: Pharmaceutical pollution alters the cost of bacterial
 infection and its relationship to pathogen load
 [https://doi.org/10.5061/dryad.6q573n63h](https://doi.org/10.5061/dryad.6q573n63h) This text document has information regarding the data files associated with Aulsebrook LC, Wong BBM & Hall MD (2023) Pharmaceutical pollution alters the cost of bacterial infection and its relationship to pathogen load. There are two CSV files in total: 1\)      Aulsebrook-2023-Offspring_Data.csv 2\)      Aulsebrook-2023-Size_Spores_Data.csv Below is a table that has the name of each data column and the corresponding definition of information within that column. | Column name | Definition | | :------------------------------------- | :------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | | Aulsebrook-2023-Offspring\_Data.csv | | | ID | A unique ID for each daphnia individual. | | Host\_genotype | The genotype of the daphnia – either HO2 (HU-HO-2, derived from a single clone from Hungary) or M10 (BE-OHZ-M10, derived from a single clone from Belgium). | | Fluoxetine\_treatment | The fluoxetine treatment that the individual was exposed to, where C = control, L = low, M = Medium and H = High. | | Pathogen | The pathogen genotype that the individual was exposed to. | | Born | The date of birth of the individual. | | Block | The experiment was performed in two blocks, which were identical but performed 2 days apart. Animals assigned to block 1 were born on 06/03 while animals assigned to block 2 were born on 08/03. | | Date | The date that offspring and status observations were recorded. | | Days\_old | How many days old the individual was on the date noted. | | Offspring | The number of offspring counted on the date noted. | | Status | Whether the individual was alive or dead on the date noted. | | Aulsebrook-2023-Size\_Spores\_Data.csv | | | ID | A unique ID for each daphnia individual. | | Host\_genotype | The genotype of the daphnia – either HO2 (HU-HO-2, derived from a single clone from Hungary) or M10 (BE-OHZ-M10, derived from a single clone from Belgium). | | Fluoxetine\_treatment | The fluoxetine treatment that the individual was exposed to, where C = control, L = low, M = Medium and H = High. | | Pathogen | The pathogen genotype that the individual was exposed to. | | Born | The date of birth of the individual. | | Block | The experiment was performed in two blocks, which were identical but performed 2 days apart. Animals assigned to block 1 were born on 06/03 while animals assigned to block 2 were born on 08/03. | | Infected | Whether the individual was infected by the pathogen. | | Status | Whether the individual was alive or dead upon completion of the experiment (i.e. after 30 days). | | Size\_mm | The body size of the individual in millimetres. | | Spore\_status | Type of spores present, where N = no spores, P = pre-spores only and M = mature spores. | | Spore\_count | The number of mature spores measured using an Accuri C6 flow cytometer. | "Missing data code NA"