cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20038274
title: RB975242 and RB975201 - Late maturation sugarcane varieties

description: Abstract The sugarcane varieties RB975201 and RB975242 were developed and released for harvest at the end of the season (late maturation) in the Central-South region of Brazil. In specific environments, these varieties were compared with commercial standards in sugar yield per area. They are resistant to major sugarcane diseases and present the Bru1 gene of resistance to brown rust.
DOI: 10.6084/m9.figshare.20038276
title: Morphological and physiological characteristics in vitro anthurium plantlets exposed to silicon

description: Abstract The objective was to evaluate morphological and physiological differences in anthurium plants in vitro, with the use of silicon added to the culture medium. Nodal segments were inoculated in Pierik with different sodium silicate concentrations (0.0, 0.5, 1.0 or 2.0 mg L-1). After 100 days in a growth room, phytotechnical characteristics were evaluated, physiological concentrations of silicon, photosynthetic pigments and microanalysis X-ray. A higher yield of chlorophyll a and b, was observed in plants supplemented with 2.0 mg L-1 of sodium silicate. Anthurium plants showed better growth development with an increase in the number of leaves, dry weight and length of the aereal part in plants supplemented with 0.5 and 2.0 mg L-1 sodium silicate. It was also observed an increase in the number of roots of plants supplemented with 1.0 mg L-1 of sodium silicate. The use of sodium silicate in culture medium improves the quality of plantlets with an increased absorption of nutrients in the plant Anthurium.
DOI: 10.6084/m9.figshare.20038276.v1
title: Morphological and physiological characteristics in vitro anthurium plantlets exposed to silicon

description: Abstract The objective was to evaluate morphological and physiological differences in anthurium plants in vitro, with the use of silicon added to the culture medium. Nodal segments were inoculated in Pierik with different sodium silicate concentrations (0.0, 0.5, 1.0 or 2.0 mg L-1). After 100 days in a growth room, phytotechnical characteristics were evaluated, physiological concentrations of silicon, photosynthetic pigments and microanalysis X-ray. A higher yield of chlorophyll a and b, was observed in plants supplemented with 2.0 mg L-1 of sodium silicate. Anthurium plants showed better growth development with an increase in the number of leaves, dry weight and length of the aereal part in plants supplemented with 0.5 and 2.0 mg L-1 sodium silicate. It was also observed an increase in the number of roots of plants supplemented with 1.0 mg L-1 of sodium silicate. The use of sodium silicate in culture medium improves the quality of plantlets with an increased absorption of nutrients in the plant Anthurium.
DOI: 10.6084/m9.figshare.20038277
title: Adaptability, stability, and genetic divergence of conilon coffee in Alto Suaçuí, Minas Gerais, Brazil

description: Abstract The objective of this study was to describe the genetic parameters, adaptability, and stability of clones from the Coffea canephora variety ‘Vitória Incaper 8142’, and the genetic divergence between them. Following 12 and 24 months of plant growth, several traits were evaluated, including stem diameter, plant height, canopy diameter, number of plagiotropic branches, and number of nodes per plagiotropic branch. The agronomic performance of four crops was determined using measurements of productivity, yield, percentage of floating grains, and grade of the beans. Variability was observed between the clones for the majority of the traits measured. The clones V7, V10, V12, and V13 were the most stable and displayed the highest degree of adaptation for the studied traits, and thus these clones will be used to establish a base population suited to the growing conditions in Alto Suaçuí, Minas Gerais, Brazil. Additionally, the genetic dissimilarity identified between clones allowed suitable clone combinations to be proposed for use in future crosses.
DOI: 10.6084/m9.figshare.20038277.v1
title: Adaptability, stability, and genetic divergence of conilon coffee in Alto Suaçuí, Minas Gerais, Brazil

description: Abstract The objective of this study was to describe the genetic parameters, adaptability, and stability of clones from the Coffea canephora variety ‘Vitória Incaper 8142’, and the genetic divergence between them. Following 12 and 24 months of plant growth, several traits were evaluated, including stem diameter, plant height, canopy diameter, number of plagiotropic branches, and number of nodes per plagiotropic branch. The agronomic performance of four crops was determined using measurements of productivity, yield, percentage of floating grains, and grade of the beans. Variability was observed between the clones for the majority of the traits measured. The clones V7, V10, V12, and V13 were the most stable and displayed the highest degree of adaptation for the studied traits, and thus these clones will be used to establish a base population suited to the growing conditions in Alto Suaçuí, Minas Gerais, Brazil. Additionally, the genetic dissimilarity identified between clones allowed suitable clone combinations to be proposed for use in future crosses.
DOI: 10.6084/m9.figshare.20038280
title: Combining ability of sugarcane genotypes based on the selection rates of single cross families

description: Abstract This study evaluated the genetic potential of parents used in sugarcane genetic breeding programs based on the performance of previously conducted single crosses. The average selection rate of each family, predicted using Best Linear Unbiased Prediction (BLUP) procedure, was used as a surrogate to the cross performance in the initial evaluation phase. Data analysis was performed using Griffing’s method IV adapted for the available set of crosses to detail the general combining ability (GCA) and specific combining ability (SCA) effects. Significant GCA effects were detected, which demonstrated the possibility of selecting parents based on this parameter. SCA had a higher coefficient of determination than GCA. In conclusion, the selection rate is an effective indicator for evaluation of the combining ability of parents in the first selection stage of a sugarcane breeding program.
DOI: 10.6084/m9.figshare.20038280.v1
title: Combining ability of sugarcane genotypes based on the selection rates of single cross families

description: Abstract This study evaluated the genetic potential of parents used in sugarcane genetic breeding programs based on the performance of previously conducted single crosses. The average selection rate of each family, predicted using Best Linear Unbiased Prediction (BLUP) procedure, was used as a surrogate to the cross performance in the initial evaluation phase. Data analysis was performed using Griffing’s method IV adapted for the available set of crosses to detail the general combining ability (GCA) and specific combining ability (SCA) effects. Significant GCA effects were detected, which demonstrated the possibility of selecting parents based on this parameter. SCA had a higher coefficient of determination than GCA. In conclusion, the selection rate is an effective indicator for evaluation of the combining ability of parents in the first selection stage of a sugarcane breeding program.
DOI: 10.6084/m9.figshare.20038282.v1
title: BRS AG: first cultivar of irrigated rice used for alcohol production or animal feed

description: Abstract BRS AG is an irrigated rice cultivar developed by Embrapa, recommended for cultivation in the state of Rio Grande do Sul. It is intended for grain alcohol production or animal feed, with average thousand-grain weight of 52g (double of the conventional cultivars) and average yield of 8193 kg ha-1.
DOI: 10.6084/m9.figshare.20038282
title: BRS AG: first cultivar of irrigated rice used for alcohol production or animal feed

description: Abstract BRS AG is an irrigated rice cultivar developed by Embrapa, recommended for cultivation in the state of Rio Grande do Sul. It is intended for grain alcohol production or animal feed, with average thousand-grain weight of 52g (double of the conventional cultivars) and average yield of 8193 kg ha-1.
DOI: 10.6084/m9.figshare.20038283
title: BRS Pampeira: new irrigated rice cultivar with high yield potential

description: Abstract BRS Pampeira is a rice cultivar developed by Embrapa, recommended for irrigated cultivation in Brazil. It shows modern architecture, with high tillering and tolerance to lodging. It stands out for its high yield potential, medium cycle and good grain quality.
DOI: 10.6084/m9.figshare.20038283.v1
title: BRS Pampeira: new irrigated rice cultivar with high yield potential

description: Abstract BRS Pampeira is a rice cultivar developed by Embrapa, recommended for irrigated cultivation in Brazil. It shows modern architecture, with high tillering and tolerance to lodging. It stands out for its high yield potential, medium cycle and good grain quality.
DOI: 10.6084/m9.figshare.20038285
title: RB036088 - a sugarcane cultivar for mechanical planting and harvesting

description: Abstract The sugarcane cultivar RB036088 is late-maturing, harvested from September to November in south-central Brazil, and is recommended for soils with medium to high fertility. It stands out with continuously high sugar yield over the harvests, longevity of the ratoon plants, high tillering capacity and can be harvested mechanically.
DOI: 10.6084/m9.figshare.20038285.v1
title: RB036088 - a sugarcane cultivar for mechanical planting and harvesting

description: Abstract The sugarcane cultivar RB036088 is late-maturing, harvested from September to November in south-central Brazil, and is recommended for soils with medium to high fertility. It stands out with continuously high sugar yield over the harvests, longevity of the ratoon plants, high tillering capacity and can be harvested mechanically.
DOI: 10.6084/m9.figshare.20038288
title: New strategy for evaluating grain cooking quality of progenies in dry bean breeding programs

description: Abstract The methodology available for evaluating the cooking quality of dry beans is impractical for assessing a large number of progenies. The aims of this study were to propose a new strategy for evaluating cooking quality of grains and to estimate genetic and phenotypic parameters using a selection index. A total of 256 progenies of the 13thcycle of a recurrent selection program were evaluated at three locations for yield, grain type, and cooked grains. Samples of grains from each progeny were placing in a cooker and the percentage of cooked grains was assessed. The new strategy for evaluating cooking quality was efficient because it allowed a nine-fold increase in the number of progenies evaluated per unit time in comparison to available methods. The absence of association between grain yield and percentage of cooked grains or grain type indicated that it is possible to select high yielding lines with excellent grain aspect and good cooking properties using a selection index.
DOI: 10.6084/m9.figshare.20038288.v1
title: New strategy for evaluating grain cooking quality of progenies in dry bean breeding programs

description: Abstract The methodology available for evaluating the cooking quality of dry beans is impractical for assessing a large number of progenies. The aims of this study were to propose a new strategy for evaluating cooking quality of grains and to estimate genetic and phenotypic parameters using a selection index. A total of 256 progenies of the 13thcycle of a recurrent selection program were evaluated at three locations for yield, grain type, and cooked grains. Samples of grains from each progeny were placing in a cooker and the percentage of cooked grains was assessed. The new strategy for evaluating cooking quality was efficient because it allowed a nine-fold increase in the number of progenies evaluated per unit time in comparison to available methods. The absence of association between grain yield and percentage of cooked grains or grain type indicated that it is possible to select high yielding lines with excellent grain aspect and good cooking properties using a selection index.
DOI: 10.6084/m9.figshare.20038289
title: Image - Rice Grain Scanner: a three-dimensional fully automated assessment of grain size and quality traits

description: Abstract The Image is a scanner developed as a grain classifier for quality control at the rice industry based on Brazilian official norms. It orders the dehulled grains ensuring that each grain would pass individually, in free fall, while the grain is analysed from different sides, covering its whole surface. It ensures a precise three-dimensional measurement of grain size, chalkiness, defects of the grain, milling quality, given out a total of 39 traits/classes/defects/values, which are sent to a excel Microsoft spreadsheet. This is managed through a digital platform, which analysis routine and layout were developed and designed by Selgron and Epagri to fit the needs of research. The scanner and its software reach outputs that enhance rice breeding efficiency for grain quality, performing it faster, precisely and with a high-throughput phenotyping than ever before, especially interesting in very early breeding generations.
DOI: 10.6084/m9.figshare.20038289.v1
title: Image - Rice Grain Scanner: a three-dimensional fully automated assessment of grain size and quality traits

description: Abstract The Image is a scanner developed as a grain classifier for quality control at the rice industry based on Brazilian official norms. It orders the dehulled grains ensuring that each grain would pass individually, in free fall, while the grain is analysed from different sides, covering its whole surface. It ensures a precise three-dimensional measurement of grain size, chalkiness, defects of the grain, milling quality, given out a total of 39 traits/classes/defects/values, which are sent to a excel Microsoft spreadsheet. This is managed through a digital platform, which analysis routine and layout were developed and designed by Selgron and Epagri to fit the needs of research. The scanner and its software reach outputs that enhance rice breeding efficiency for grain quality, performing it faster, precisely and with a high-throughput phenotyping than ever before, especially interesting in very early breeding generations.
DOI: 10.6084/m9.figshare.20038297
title: Efficient and new method for Tectona grandis in vitro regeneration

description: Abstract The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.
DOI: 10.6084/m9.figshare.20038297.v1
title: Efficient and new method for Tectona grandis in vitro regeneration

description: Abstract The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.
DOI: 10.6084/m9.figshare.20038298
title: Agrobacterium-mediated genetic transformation of a tropical elite maize line

description: Abstract The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% - half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens.
DOI: 10.6084/m9.figshare.20038298.v1
title: Agrobacterium-mediated genetic transformation of a tropical elite maize line

description: Abstract The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% - half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens.
DOI: 10.6084/m9.figshare.20038300
title: Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola)

description: Abstract Downy mildew (Plasmopara viticola) is the main grapevine disease in humid regions. In the present investigation, marker-assisted selection (MAS) was used to develop grapevine lines homozygous in loci Rpv1 and Rpv3 for resistance against P. viticola. The experimental populations UFSC-2013-1 (n = 420) and UFSC-2013-2 (n = 237) were obtained by self-pollination of two �� �� full-sib plants, originated from a cross between two distinct breeding lines containing the downy mildew resistance loci Rpv1 and Rpv3 in heterozygosity. The two experimental populations were genotyped with four microsatellite markers flanking the two downy mildew resistance loci. Among 637 genotyped plants, 300 (48.2%) were homozygous for at least one resistance locus and 10 (1.57%) were homozygous for both Rpv1 and Rpv3 alleles. These 10 plants challenged with P. viticola inoculum showed a clearly enhanced level of resistance. These plants have a great potential as resistance donors in grapevine breeding.
DOI: 10.6084/m9.figshare.20038300.v1
title: Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola)

description: Abstract Downy mildew (Plasmopara viticola) is the main grapevine disease in humid regions. In the present investigation, marker-assisted selection (MAS) was used to develop grapevine lines homozygous in loci Rpv1 and Rpv3 for resistance against P. viticola. The experimental populations UFSC-2013-1 (n = 420) and UFSC-2013-2 (n = 237) were obtained by self-pollination of two �� �� full-sib plants, originated from a cross between two distinct breeding lines containing the downy mildew resistance loci Rpv1 and Rpv3 in heterozygosity. The two experimental populations were genotyped with four microsatellite markers flanking the two downy mildew resistance loci. Among 637 genotyped plants, 300 (48.2%) were homozygous for at least one resistance locus and 10 (1.57%) were homozygous for both Rpv1 and Rpv3 alleles. These 10 plants challenged with P. viticola inoculum showed a clearly enhanced level of resistance. These plants have a great potential as resistance donors in grapevine breeding.
DOI: 10.6084/m9.figshare.20038303
title: Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense

description: Abstract The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids. For meiotic analysis and meiotic index, flower buds were collected and fixed for 24 hours in ethanol: acetic acid (3:1), and then transferred to 70% ethanol, and stored in a freezer. Slides preparation for meiosis, meiotic index and pollen viability was carried out according to the laboratory’s protocol. Hybrid meiosis was regular, and 12 pairs of chromosomes were observed in diakinesis, as well as a few anomalies. The MI of 88.16%, as well as the pollen viability of 72.5%, were satisfactory. Given the results, it was confirmed the genetic proximity of these species, which are classified in the Capsicum annuum gene complex.
DOI: 10.6084/m9.figshare.20038303.v1
title: Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense

description: Abstract The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids. For meiotic analysis and meiotic index, flower buds were collected and fixed for 24 hours in ethanol: acetic acid (3:1), and then transferred to 70% ethanol, and stored in a freezer. Slides preparation for meiosis, meiotic index and pollen viability was carried out according to the laboratory’s protocol. Hybrid meiosis was regular, and 12 pairs of chromosomes were observed in diakinesis, as well as a few anomalies. The MI of 88.16%, as well as the pollen viability of 72.5%, were satisfactory. Given the results, it was confirmed the genetic proximity of these species, which are classified in the Capsicum annuum gene complex.
DOI: 10.6084/m9.figshare.20038304
title: An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR

description: Abstract An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris.