cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/5yzyc-b8v97
title: Vibrio harveyi

description: wild type Vibrio harveyi BB120
description: <strong>Tilt Series Date:</strong> 2013-12-24</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio harveyi</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Kirsten Jung</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in ab medium</p>
  aba2013-12-24-10_slicer3626.jpg: Vibrio harveyi pilus aba2013-12-24-10_slicer3627.jpg: Vibrio harveyi pilus aba2013-12-24-10_slicer3628.jpg: Vibrio harveyi filament bundle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-24-10/rawdata/</p>BB120VibrioHWT0010.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-24-10/rawdata/BB120VibrioHWT0010.mrc"
        > <i class="download icon"></i> Download </a></p>

        BB120VibrioHWT0010SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-24-10/3dimage_46422/BB120VibrioHWT0010SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/966en-x7x15
title: M-MuLV 43D infected MEFs

description: M-MuLV 43D wildtype
description: <strong>Tilt Series Date:</strong> 2013-11-19</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> M-MuLV infected cells provided by Hung Fan (UC Irvine)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole MEFs infected with M-MuLV 43D were cultured on Au grids, blotted manually and plunge frozen.</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-19-6/3dimage_34537/</p>2013nov19_C183_1_43D_at1bt1_0004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-19-6/3dimage_34537/2013nov19_C183_1_43D_at1bt1_0004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        2013nov19_C183_1_43D_at1bt1_0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-19-6/rawdata/2013nov19_C183_1_43D_at1bt1_0004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/srnn8-5bs84
title: UU 2804- A413T CheA deltaP2

description: E. coli strain A413T (locked in CheA off state) with deletion of P2 in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-31</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo/IMOD/tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB media, lysed with penicillin for one hour</p>
<strong>Sample Preparation:</strong> plunge frozen on R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-13/rawdata/</p>2804atlas10013.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-13/rawdata/2804atlas10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        2804atlas10013_part130_62.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-13/3dimage_20596/2804atlas10013_part130_62.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c6scr-hx054
title: HUVECs infx pNLGFPBglI

description: Raw data files of HUVECs infx pNLGFPBglI
description: <strong>Tilt Series Date:</strong> 2013-08-17</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFPBglI obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen 24h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-08-17-4/rawdata/</p>13aug17_C167_1_HUVECspNLBglI224hPI_AT1BT1_0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-08-17-4/rawdata/13aug17_C167_1_HUVECspNLBglI224hPI_AT1BT1_0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        13aug17_C167_1_HUVECspNLBglI224hPI_AT1BT1_0004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-08-17-4/3dimage_29726/13aug17_C167_1_HUVECspNLBglI224hPI_AT1BT1_0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zpava-jfe91
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-13</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-33/rawdata/</p>12mar13_c121_1_Cos74ADN_AT1bt1_0027.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-33/rawdata/12mar13_c121_1_Cos74ADN_AT1bt1_0027.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar13_c121_1_Cos74ADN_AT1bt1_0027_part68_6.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-33/3dimage_17839/12mar13_c121_1_Cos74ADN_AT1bt1_0027_part68_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/762h1-t9s67
title: V. cholerae delta CheA1,2,3

description: Vibrio cholerae strain deleted for CheA1, CheA2, and CheA3
description: <strong>Tilt Series Date:</strong> 2014-05-05</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Ikuro Kawagishi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C</p>
  aba2014-05-05-6_slicer3869.jpg: DNA filament bundles?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-05-05-6/3dimage_42164/</p>VibrioDelta123May0006_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-05-05-6/3dimage_42164/VibrioDelta123May0006_full.rec"
        > <i class="download icon"></i> Download </a></p>

        VibrioDelta123May0006.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-05-05-6/rawdata/VibrioDelta123May0006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0fv4z-0y698
title: Alongum

description: Alongum_periplasmiclayers
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-65/rawdata/</p>alongLys0006.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-65/rawdata/alongLys0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        alongLys0006_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-65/3dimage_56848/alongLys0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ep91b-zqq55
title: HUVECs infx pNLBg1I_endptvirus

description: Raw data files of HUVECs infx pNLBg1I_endptvirus
description: <strong>Tilt Series Date:</strong> 2013-11-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLGFPBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBg1I. Sample was frozen after endpoint of virus infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-40/rawdata/</p>13nov04_Endptvirus_montage_21x21tgt_0025.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-40/rawdata/13nov04_Endptvirus_montage_21x21tgt_0025.mrc"
        > <i class="download icon"></i> Download </a></p>

        13nov04_Endptvirus_montage_21x21tgt_0025_part121_3.rec,  Reconstruction (Pixel Size 2.24 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-40/3dimage_33899/13nov04_Endptvirus_montage_21x21tgt_0025_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/grcpf-xw953
title: UU 2804- A413T CheA deltaP2

description: E. coli strain A413T (locked in CheA off state) with deletion of P2 in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-31</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo/IMOD/tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB media, lysed with penicillin for one hour</p>
<strong>Sample Preparation:</strong> plunge frozen on R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-6/rawdata/</p>2804atlas10006.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-6/rawdata/2804atlas10006.mrc"
        > <i class="download icon"></i> Download </a></p>

        2804atlas10006_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-6/3dimage_20582/2804atlas10006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7011r-pxh64
title: Agro Bald VirB8-GFP

description: the bald strain lacks flagellin 1-3 and is considered non-motile
description: <strong>Tilt Series Date:</strong> 2013-07-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown at 19C and induced for T4SS</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-15/rawdata/</p>bald_gfp20015.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-15/rawdata/bald_gfp20015.mrc"
        > <i class="download icon"></i> Download </a></p>

        bald_gfp20015_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-15/3dimage_27825/bald_gfp20015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dbwby-cpt95
title: PAO1 Wild Type

description: Pseudomonas aeruginosa PAO1 Wild Type
description: <strong>Tilt Series Date:</strong> 2014-10-14</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-25/rawdata/</p>PAOIWTMCTest0015.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-25/rawdata/PAOIWTMCTest0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        PAOIWTMCTest0015_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-25/3dimage_47065/PAOIWTMCTest0015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v7nqz-dar96
title: E.coli "strain 3" G235E CheA on

description: chromosomal chemoreceptor mutant G235E "CheA on"
description: <strong>Tilt Series Date:</strong> 2012-02-10</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sandy Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> culture grown in TB medium (+GEntamycin) to OD (600) 0.31 at 30 degrees. Additional one hour incubation with penicillin before freezing.</p>
<strong>Sample Preparation:</strong> freezing with Vitrobot @ 100 % humidity, -4 offset, 3.5 s blot time, no drain, 3 µl sample, pure ethane.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-2/rawdata/</p>strain30002.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-2/rawdata/strain30002.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain30002_part.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-2/3dimage_16330/strain30002_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rbtef-sv372
title: Agrobacterium tumefaciens

description: Agrao VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-08</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-55/rawdata/</p>agro_50005.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
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        agro_50005_part121_24.rec,  Reconstruction (Pixel Size 0.0 nm), 516.7 MB  
        
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