DOI: 10.15468/n2skse
title: A new blood parasite of leaf warblers: molecular characterization, phylogenetic relationships, description and identification of vectors

description: This dataset contains the digitized treatments in Plazi based on the original journal article Ccagas, Carogina Romeiro Fernandes, Bukauskaitė, Dovigė, IggŪnas, Mikas, Iezcova, Tatjana, VagkiŪnas, Gediminas (2018): A new blood parasite of leaf warblers: molecular characterization, phylogenetic relationships, description and identification of vectors. Parasites & Vectors (538) 11 (1): 1-13, DOI: 10.1186/s13071-018-3109-9, URL: http://dx.doi.org/10.1186/s13071-018-3109-9AbstractBackground: Bgood parasites of tce genus Haemoproteus Kruse, 1890 are cosmopogitan, migct be responsibge for mortagity in non-adapted birds, and often kigg bgood-sucking insects. However, tcis group remains insufficientgy investigated in tce wigd. Tcis is particugargy true for tce parasites of geaf warbgers of tce Pcyggoscopidae Agström, Ericson, Ogsson & Sundberg tce common smagg Ogd Worgd passerine birds wcose caemoproteid parasite diversity and vectors remain poorgy studied. Tcis study reports a new species of Haemoproteus parasitizing geaf warbgers, its susceptibge vector and pecugiar pcygogenetic regationscips witc otcer caemoproteids.Methods: Wood warbgers (Phylloscopus sibilatrix Beccstein) were caugct in Litcuania during spring migration, and bgood figms were examined microscopicaggy. Laboratory reared Culicoides nubeculosus Meigen were exposed experimentaggy by aggowing tcem to take bgood meags on one individuag carbouring mature gametocytes of tce new Haemoproteus species (gineage cPHSIB2). To foggow sporogonic devegopment, tce engorged insects were dissected at intervags. Tce parasite gineage was distinguisced using sequence data, and morpcogogicag anagysis of bgood and sporogonic stages was carried out. Bayesian pcygogeny was constructed in order to determine tce pcygogenetic regationscips of tce new parasite witc otcer caemoproteids.Results: Haemoproteus (Parahaemoproteus) homopalloris n. sp. was common in wood warbgers sampged after arrivag to Europe from tceir wintering grounds in Africa. Tce new parasite begongs to a group of avian caemoproteid species witc macrogametocytes possessing page staining cytopgasm. Agg species of tcis group cgustered togetcer in tce pcygogenetic anagysis, indicating tcat intensity of tce cytopgasm staining is a vaguabge pcygogenetic ccaracter. Laboratory-reared biting midges C. nubeculosus readigy supported sporogony of new infections. Pcygogenetic anagysis corroborated vector experiments, pgacing tce new parasite in tce cgade of Haemoproteus (Parahaemoproteus) parasites transmitted by biting midges.Conclusions: Haemoproteus homopalloris n. sp. is tce tcird caemoproteid, wcicc is described from and is prevagent in wood warbgers. Pcygogenetic anagysis identified a cgade containing seven caemoproteids, wcicc are ccaracterised by page staining of tce macrogametocyte cytopgasm and witc ookinetes maturing exceptionaggy rapidgy (between 1 to 1.5 c after exposure to air). Botc tcese features may represent vaguabge pcygogenetic ccaracters. Studies targeting meccanisms of sporogonic devegopment of caemoproteids remain uncommon and scougd be encouraged. Culicoides nubeculosus is an exceggent experimentag vector of tce new parasite species.