DOI: 10.5061/dryad.5x69p8d0x
title: Low host specificity and broad geographic ranges in a community of parasitic non-pollinating fig wasps (Sycoryctinae; Chalcidoidea)

description: Three non-pollinating chalcid fig-wasp species have been reported from a
 dioecious fig, Ficus hirta Vahl., Philotrypesis josephib (tribe
 Philotrypesini), Sycoscapter hirticola and Sycoryctes sp. (tribe
 Sycoryctini). They oviposit into ovaries containing Valisia larvae by
 inserting their long ovipositor through the fig
 wall. Microsatellites across 24 geographical sites for these two
 tribes were collected to establish species genetic diversity, inbreeding
 and species boundaries. We sampled representatives of each fig wasp
 species emerging from individual figs at each location. For Philotrypesis,
 262 individuals, each from a different fig, were genotyped at 6 unlinked
 microsatellite loci. The genotype data of Philotrypesis is in the sheet 1
 with detailed sampling information and nuclear locus. For Sycoryctini, 203
 individuals, each from a different fig, were genotyped at 7 unlinked
 microsatellite loci. The genotype data of Sycoryctini is in the sheet 2
 with detailed sampling information and nuclear locus.
description: Samples were collected from South China to Java (Supporting
 Information Table S1). In each location, 10-30 mature figs were collected
 just before wasp emergence. The ﬁgs were placed in ﬁne-mesh bags to allow
 the wasps to emerge naturally. The wasps that emerged were preserved in
 95% ethanol and stored at -20°C until DNA extraction. For microsatellite data, a single
 female wasp per fig was genotyped for both
 <i>Philotrypesis</i> and Sycoryctini. For
 <i>Philotrypesis</i>, 262 individuals were genotyped at 6
 unlinked microsatellite loci (P50, P86, P91, P123, P129 and P202) that had
 been developed for <i>F. hirta</i>
 <i>Philotrypesis</i> [54]. For
 <i>Sycoscapter-Sycoryctes</i>, 203 individuals were genotyped
 at 7 unlinked microsatellite loci (S7, S27, S52, S122, S132, S137 and
 S162) that had been developed for <i>F. hirta Sycoscapter</i>
 [55]. Molecular techniques followed Abellò et al. [54] and Ahanchédé et
 al. [55]. Method
 of microsatellite amplification for Sycorictini and
 <i>Philotrypesis</i> were the same expect for the annealing
 temperature. The amplification reactions were conducted using a PTC-200
 thermal cycler (Bio-Rad, Hercules, CA) in 20 µl volume containing 20 ng of
 genomic DNA, 0.2 mM of each dNTP, 0.4 µM of fluorescent primer, 1× PCR
 buffer (Mg2+ free), 2.5 mM Mg2+ and 1 unit of Taq DNA polymerase (Takara,
 Dalian, China), using the following conditions: initial denaturation at
 95°C for 5 min, 35 cycles of (94°C, 30 s; 46 to 50°C for Sycoryctini and
 47 to 52 °C for <i>Philotrypesis</i>, 60 s; 72°C, 45 s) and a
 final extension of 72°C for 8 min. The fragment sizes of the PCR products
 were determined on the ABI PRISM 3100 Genetic Analyser (Applied
 Biosystems, Foster City, CA) using genotyper 4.0 and LIZ 500 (Applied
 Biosystems, Foster City, CA) as an internal size standard.
description: Microsatellite genotype data for all individuals of
 <i>Philotrypesis</i> and
 <em>Syocryctini</em>: Sheet 1: Genotypic
 data of <i>Philotrypesis</i> Nuclear
 genotypes (6 loci) for 262 individuals of
 <i>Philotrypesis</i>. Population refers to collection sites,
 with GPS coordinates provided in the main text. For each nuclear locus,
 alleles names are arbitrary (i.e., do not indicate size or sequence
 characteristics), and “0” indicates missing data. Sheet
 2: Genotypic data of <i>Sycorictini</i>
 Nuclear genotypes (7 loci) for 203 individuals of<i>
 Sycorictini</i>. Population refers to collection sites, with GPS
 coordinates provided in the main text. For each nuclear locus, alleles
 names are arbitrary (i.e., do not indicate size or sequence
 characteristics), and “0” indicates missing data.