DOI: 10.5061/dryad.fj6q57426
title: Quantitative disease resistance in wild Silene vulgaris to its endemic pathogen Microbotryum silenes-inflatae collection sites

description: Details presented on the collections sites of Silene vulgaris seeds from
 natural populations, for use in the study, Quantitative Disease Resistance
 in wild Silene vulgaris to its Endemic Pathogen Microbotryum
 silenes-inflatae.  In this study the seeds were collected a mixed
 half and full sibling families (seeds from the same maternal plants) and
 used to produce plants that were then cloned by vegetative
 cuttings.  The infection rates of these clones, as well as of
 their F1 offspring, by the fungus Microbotryum silenes-inflatae are also
 presented.
description: Surveys of natural populations of <em>Silene
 vulgaris</em> identified sites of seed collection.  Data on the
 susceptibility to the fungal pathoge, <em>Microbotryum
 silenes-inflatae</em>, were collected from greenhouse experiments,
 including the susceptiblity of the field-collected seed genotypes based on
 growing the plants and producing multiple clones (by vegetative
 propogation) prior to inoculation (data show the proportion of clones that
 became diseased), and including susceptibility of F1 generation produced
 in the greenhouse from those genotypes and then inoculating the offspring
 (data showing the proportion of F1 offspring that became diseased).  Data
 presented are not transformed.
description: # Quantitative Disease Resistance in wild Silene vulgaris to its Endemic
 Pathogen Microbotryum silenes-inflatae Collection Sites
 The data describe the
 collection sites and production of cloned offspring for the plant *Silene
 vulgaris*.. ## Description of the data and file structure The information
 is provided in three cvs tables, found on three files as untransformed
 data. The first file (titled: Field Sites for Seed Collection) contains
 the plant families from collection sites identified by the corresponding
 names in the column "field-collected family IDs". The
 field-collected families each represent mixtures of full and half-sibling
 groups as the seeds collected from the same maternal plant. The family IDs
 were given alphanumeric, single character names ranging from A-Z and 0-9,
 where families with sufficient seeds were included in the current study.
 Seeds from these families, representing individual plant genotypes, were
 used to produce clones by vegetative propagation. In addition, the
 "Number of Genotypes Cloned" the "Average Number of Clones
 per Genotype" and standard deviation are given. The second file
 (titled: Infect. Field Collect Families) includes the plant families
 identified by the corresponding names in the column "Field-Collected
 Family ID", that are the same as the family names in first data file
 (titled: Field Sites for Seed Collection); field-collected families each
 represent mixtures of full and half-sibling groups as the seeds collected
 from the same maternal plant. The family IDs were given alphanumeric,
 single character names ranging from A-Z and 0-9, where families with
 sufficient seeds were included in the current study. The "Genotype ID
 within Family" representing the plant derived from an individual
 seed, "Clone Count per Genotype", "Number Infected"
 indicating the number of clones that became diseased following
 inoculation, and "Proportion Diseased" among those clones. The
 third file (titled: Infec F1 Offspring) includes "Replicate",
 "Resistance Quartile of Maternal Parent", "Maternal
 Family", "Maternal Parent Genotype", "F1 Offspring
 Count", "Number Infected", and "Proportion
 Diseased". These data show the results of the infection experiment
 with the F1 offspring from field-collected families shown in the first and
 second data files (titled: Field Sites for Seed Collection, and titled:
 Infect. Field Collect Families). "Replicate" indicates the two
 replicates runs of the experiment. The maternal parents of the F1
 offspring were ranked according to their susceptibility to infection (as
 shown in the second worksheet, titled: Infect. Field Collect Families),
 and grouped into ranked resistance quartiles that are indicated by
 "Resistance Quartile of Maternal Parent" with value of 1 as the
 most resistant and 4 as the most susceptible. "Maternal Family"
 corresponds to the field-collected family to which the maternal parent of
 the F1 offspring belonged (identified in the second data file titled:
 Field-Collected Family ID); field-collected families each represent
 mixtures of full and half-sibling groups as the seeds collected from the
 same maternal plant. "Maternal Parent Genotype" corresponds to a
 combination code that adds together the "Maternal Family" and
 the number identifying the plant derived from an individual seed, shown as
 the "Genotype ID within Family" from the second worksheet
 (titled: Field-Collected Family ID). "F1 Offspring Count" shows
 the number of F1 offspring that were included in inoculation experiment.
 "Number Infected" indicates the number of F1 offspring that
 became diseased following inoculation. "Proportion Diseased"
 shows the number that became infected divided by the number of F1
 offspring that were included in the study. ## Sharing/Access information
 These data are associated with the in-progress publication
 "Quantitative Disease Resistance in wild Silene vulgaris to its
 Endemic Pathogen Microbotryum silenes-inflatae."