DOI: 10.5281/zenodo.7574004
title: Consensus molecular environment of schizophrenia risk genes in co-expression networks shifting across brain development, age and region

description: This is the online data repository accompanying the following manuscript:<br> <strong>Consensus molecular environment of schizophrenia risk genes in co-expression networks shifting across brain development, age and region</strong> <em>Giulio Pergola<sup>1,2,*</sup>, Madhur Parihar<sup>1</sup>, Leonardo Sportelli<sup>1,2</sup>, Rahul Bharadwaj<sup>1</sup>, Christopher Borcuk<sup>2</sup>, Eugenia Radulescu<sup>1</sup>, Loredana Bellantuono<sup>2,5</sup>, Giuseppe Blasi<sup>2,3</sup>, Qiang Chen<sup>1</sup>, Joel E. Kleinman<sup>1,4</sup>, Yanhong Wang<sup>1</sup>, Srinidhi Rao Sripathy<sup>1</sup>, Brady J. Maher<sup>1,4,7</sup>, Alfonso Monaco<sup>5,9</sup>, Fabiana Rossi<sup>1,2,10</sup>, Joo Heon Shin<sup>1</sup>, Thomas M. Hyde<sup>1,4,6</sup>, Alessandro Bertolino<sup>2,3,*</sup>, Daniel R. Weinberger<sup>1,7,8,*</sup></em> <strong>Affiliations:</strong> <em>1</em><em>)Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD (USA)</em> <em>2)Group of Psychiatric Neuroscience, Department of Translational Biomedicine and Neuroscience, University of Bari Aldo Moro, Bari, Italy</em> <em>3)</em><em>Azienda Ospedaliero-Universitaria Consorziale Policlinico, Bari, Italy</em> <em>4)Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland </em> <em>5)Istituto Nazionale di Fisica Nucleare (INFN), Bari, Italy</em> <em>6)Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland</em> <em>7)</em><em>Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland</em> <em>8)Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland</em> <em>9)Dipartimento Interateneo di fisica, Università degli Studi di Bari Aldo Moro, Bari, Italy<br> 10)Campus Bio-Medico University of Rome, Rome, Italy</em> <strong>Abstract:</strong> <em>Schizophrenia is a neurodevelopmental brain disorder whose genetic risk is associated with shifting clinical phenomena across the lifespan. We investigated the convergence of putative schizophrenia risk genes in brain co-expression networks in postmortem human prefrontal cortex (DLPFC), hippocampus, caudate nucleus and dentate gyrus granule cells, parsed by specific age periods (total N=833). The results support an early prefrontal involvement in the biology underlying schizophrenia, while also revealing a dynamic interplay of regions in which age-parsing explains more variance in schizophrenia risk compared to lumping all age periods together. Across multiple data sources and publications, we identify 28 genes that are the most consistently found partners in modules enriched for putative schizophrenia risk genes in DLPFC; 21 are novel associations with schizophrenia. In iPSC-derived neurons, the unique relationship of these genes with putative schizophrenia risk genes is maintained. The genetic architecture of schizophrenia is embedded in shifting co-expression patterns across brain regions and across time, potentially underwriting its shifting clinical presentation.</em> <strong>Data Files:<br> DLPFC hit.genes_kb_200__online.version.zip: </strong><br> Interactive Sankey plot for age-parsed DLPFC networks with SCZ genes (200 kbp list) only. For Sankey plots, hover mouse over the links to see the list of genes. Also supports zoom, drag and selection.<br> <strong>DLPFC hit.genes_kb_200__paper.version.zip:</strong><br> Interactive Sankey plot for age-parsed DLPFC networks with SCZ genes (200 kbp list) only. For paper version of the figure, smaller modules are merged into a macro-module (lightgrey color)<br> <strong>DLPFC all.genes_kb_200__online.version.zip:</strong><br> Interactive Sankey plot for age-parsed DLPFC networks with all genes<br> <strong>DLPFC all.genes_kb_200__paper.version.zip:</strong><br> Interactive Sankey plot for age-parsed DLPFC networks with all genes. For paper version of the figure, smaller modules are merged into a macro-module (lightgrey color)<br> <strong>HP hit.genes_kb_200__online.version.zip:</strong><br> Interactive Sankey plot for age-parsed Hippocampus networks with SCZ genes (200 kbp list) only<br> <strong>HP hit.genes_kb_200__paper.version.zip:</strong><br> Interactive Sankey plot for age-parsed Hippocampus networks with SCZ genes (200 kbp list) only. For paper version of the figure, smaller modules are merged into a macro-module (lightgrey color)<br> <strong>HP all.genes_kb_200__online.version.zip:</strong><br> Interactive Sankey plot for age-parsed Hippocampus networks with all genes<br> <strong>HP all.genes_kb_200__paper.version.zip:</strong><br> Interactive Sankey plot for age-parsed Hippocampus networks with all genes. For paper version of the figure, smaller modules are merged into a macro-module (lightgrey color)<br> <strong>Modulewise SCZ enrichment.xlsx:</strong><br> Excel file contains module level SCZ enrichment results for all networks<br> <strong>wide_form_test_slidingwindow_NC_SchizoNew(v1.4)_final.xlsx:</strong><br> Excel file contains WGCNA output for sliding window networks<br> <strong>wide_form_WGCNA(v3.7.1)_final.xlsx:</strong><br> Excel file contains WGCNA output for our generated networks and from previously published networks<br> <strong>libdnetworks(NC).preprocessed.RData: </strong><br> Preprocessed data for parsed/nonparsed NC networks (DLPFC, HP, CAUDATE, DENTATE). For fixed window and sliding window study. [SummarizedExperiment objects]<br> <strong>libdnetworks(SCZ).preprocessed.RData: </strong><br> Preprocessed data for nonparsed SCZ networks (DLPFC, HP, CAUDATE, DENTATE). For the sliding window study. [As “SummarizedExperiment” objects in R]<br> <strong>sample_matched_HP_DG_qsva(NC).preprocessed.RData:</strong><br> Preprocessed data for the sample-matched HP-DG. QSVA removed pipeline. For Cell population enrichment study. [As “SummarizedExperiment” objects in R]<br> <strong>sample_matched_HP_DG_noqsva(NC).preprocessed.RData:</strong><br> Preprocessed data for the sample-matched HP-DG. No QSVA removed pipeline. For Cell population enrichment study. [As “SummarizedExperiment” objects in R]<br> <strong>stemcell.preprocessed.RData:</strong><br> Preprocessed data for the iPSC network. For replication in human iPSC data study. Neuronal samples averaged for each “RealGenome”. [As “SummarizedExperiment” objects in R]<br> Accompanying code can be found at: https://github.com/LieberInstitute/Brain_WGCNA<br> Data from this repository is also available at: https://nets.libd.org/age_wgcna/ For any data inquiries please contact:<br> <strong>Giulio Pergola: </strong><strong>Giulio.Pergola@libd.org</strong>
description: Change log: 1) Added online/paper versions of sankey plots 2) Added SCZ enrichment excel 3)Added prepocessed RData files for studies in this manuscript