cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0gf0s8c
title: CIL:39502, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A cross-section and tangential views of three other linkage complexes. The mid piece sits in an indentation of the ER membrane on a bed of 5 slats that pass through the membrane. Rails affix the ER membrane to the myoneme and prevent the vesiculated ER from moving away from the myoneme. Beautifully preserved views of cross sections of cilia. TEM taken on 3/7/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 28,500X. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j05h7fx9
title: CIL: 54445, Homo sapiens. In Cell Image Library

description: Imaging mode: bright-field microscopy
Visualization methods: silver nitrate and ammonium hydroxide
description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human non-symptomatic donor labeled by Bielschowsky's silver stain.
DOI: 10.6075/j0jm29c1
title: CIL: 54572, Xenopus Laevis, Entire Organism. In Cell Image Library

description: Video of African clawed frog embryonic development. Video filename format is as follows organism_temperature_batch. Data was collected at 15.2 degrees Celcius
description: Imaging mode: RGB
Visualization methods: Captured on a microscope using a Canon Rebel Ti5/6 or a one of the following eyepiece cameras: Swiftcam 3 Megapixel, OMAX 9.0 MP, AmScope MU300
description: 15.2 degrees C
DOI: 10.6075/j0ng4p90
title: CIL:37336, epidermal cell. In Cell Image Library

description: This video shows a cytoplast in the process of forming. Early in the sequence the cytoplast is still attached by a retraction fiber to its parent cell. As the retraction fiber breaks, the cytoplast is suddenly released from the parent cell. The cytoplast then reabsorbs the retraction fiber and migrates autonomously. Video made available thru Mark Cooper and Zebrafish-The Living Laboratory.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw
DOI: 10.6075/j0xd116f
title: CIL: 52313, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Tile x=4, y=2, z=15 from BL_024216_Myc_Terc
description: One z layer of a z-stack at the given tile position
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
DOI: 10.6075/j03r0rp1
title: CIL:41473, coral, polyp. In Cell Image Library

description: Scanning electron micrograph of the calcium skeleton of coral polyp. The sample was cleaned with bleach solution, dried and carbon coated prior to imaging. The image was collected using a secondary electron detector on the scanning electron microscope, which was operated at 10kV. The original magnification of the image was 100X.
description: Preparation: bleach
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: secondary_electron imaging
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
DOI: 10.6075/j0bc3xtd
title: CIL:1117, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
DOI: 10.6075/j0f47p51
title: CIL:39651, Saccharomyces cerevisiae. In Cell Image Library

description: Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo
PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage, automatic focusing, motorized illumination shutters and a CoolSNAP_HQ2 camera.
description: 10-hour timelapse of Wild-type Saccharomyces cerevisiae growing in 2% raffinose. These experiments are the control experiments used for comparison to the effects of the double mutant Clb2dbŒî, Clb5Œî on the cell cycle control system in budding yeast. The images from two experiments done with Wild-type Saccharomyces cerevisiae are available as CIL:39606-39625 and CIL:39646-39665. The images from two experiments done with the double mutant Clb2dbŒî, Clb5Œî are available as CIL:39626-39645 and CIL:39666-39685.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j0251hf6
title: CIL:2286, Caenorhabditis elegans. In Cell Image Library

description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 6 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j0nz87s7
title: CIL: 54840, Conchiformibius steedae, bacteria. In Cell Image Library

description: Leica TCS SP8 X; 63X Plan-Apochromat glycerine objective with a NA of 1.30 and a refraction index of 1.46.
description: Imaging mode: confocal microscopy
Visualization methods: fluorescent d amino acids
description: Screnshot showing the position of a synthesized septum within the C. steedae filament.
DOI: 10.6075/j08w3c4c
title: CIL:39053, Mus musculus, stem cell, goblet cell. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: unprocessed raw data
description: B0007616 Mouse colon. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: This fluorescent micrograph shows stem cells labeled with green fluorescent protein (GFP) located at the base of the colonic crypt in a section of mouse colon. The stem cells are surrounded by numerous goblet cells that secrete mucous (stained in red). Other cells of the colon are observed using DAPI staining, which highlights the cell nucleus in blue.
DOI: 10.6075/j0k0732h
title: CIL:11226, Mus musculus, hepatocyte. In Cell Image Library

description: Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: negative staining
Data qualification: Processed
description: Gap junctions isolated from mouse liver and negatively stained with uranyl formate. At higher magnification, the 8-9 nm particles, or connexons, have a central 1.5-2 nm electron dense pore. Images by Bernard Gilula appear as Figures 97 (upper) and 98 (lower) from Chapter 3 (Junctional Specializations) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0dj5dz3
title: CIL:40371, Drosophila melanogaster. In Cell Image Library

description: Images collected on a JEOL 1010 TEM.
For additional technical details see:
Webber, H.A, Howard, L., Bickeyl, S.E. 2004. The cohesion protein ORD is required for homologue bias during meiotic recombination. JCB Vol 164 (6): 819-829
description: Transmission electron microscope image of a region in the Drosophila germarium. Specific cells in the germarium contain synaptonemal complex in their nuclei.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j0gt5n7z
title: CIL:36321, Rattus, astrocyte. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed
description: Male Sprague Dawley rats, 3 weeks of age, were anaesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy,cells with a small diameter soma (approx. 7–11µm) in the stratum
radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using a 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 3 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled to produce this maximum intensity projection image.
DOI: 10.6075/j0dz07tg
title: CIL: 52403, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given tile position
description: Tile x=4, y=5, z=21 from BL024199_Myc_Terc
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
DOI: 10.6075/j0rr1x29
title: CIL:35977, Cavia porcellus, epithelial cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 355 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Glycogen-storing cells of a tubulus rectus of the guinea pig testis. Image from Fawcett and Dym, J. Reprod. Fertil., 38:401-409, 1974. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.5878/kp2z-2239
title: Hörsel- och bullerdata från svenska förskolor
title: Hearing and Noise Data from Swedish Preschools
title: pDPOAE and Noise Exposure in Swedish Preschools

description: This dataset contains anonymised measurements of hearing function and noise exposure among children in Swedish preschools. Data collection involved two types of objective measurements:

Pressurised Distortion Product Otoacoustic Emissions (pDPOAE) as an indicator of cochlear function.

Noise exposure measurements using personal dosimeters that recorded A-weighted sound levels during preschool attendance.

No tympanometry data are included in the dataset, but tympanometric screening was used as a quality control criterion for inclusion of DPOAE results.

The dataset includes the following variables:

Anonymised participant ID

Measurement timing (morning/afternoon, beginning/end of the week)

LAeqTi (equivalent continuous A-weighted sound level over the measurement interval)

LAFmax95 (95th percentile of maximum A-weighted sound pressure level)

DPOAE amplitudes for the right and left ears at 3–8 kHz, quality controlled according to predefined criteria

Variable definitions, units, and coding schemes are provided in the accompanying codebook.
description: Detta datamaterial innehåller anonymiserade mätningar av hörselfunktion och ljudnivåexponering hos barn i svenska förskolor. Datainsamlingen omfattade två typer av objektiva mätningar:

Pressurised Distortion Product Otoacoustic Emissions (pDPOAE) som indikator på cochleär funktion.

Ljudnivåmätningar med hjälp av personburna dosimetrar som registrerade A-vägda ljudnivåer under förskolevistelsen.

Ingen tympanometridata ingår i datasetet, men tympanometrisk screening användes som kvalitetskriterium för inkludering av DPOAE-resultat.

Datasetet innehåller följande variabler:

Anonymiserat ID

Tidpunkt för mätning (morgon/eftermiddag, början/slut av vecka)

LAeqTi (ekvivalent kontinuerlig ljudnivå under mätperioden)

LAFmax95 (95:e percentilen av maximal A-vägd ljudnivå)

DPOAE-amplituder i höger och vänster öra vid 3–8 kHz, kvalitetssäkrade enligt fördefinierade kriterier

 Variablernas definitioner, enheter och kodningar framgår i den bifogade variabelförteckningen
DOI: 10.6075/j0qv3kd4
title: CIL:50612, Homo sapiens, monocyte. In Cell Image Library

description: Cerebrospinal fluid, cytocentrifugation, May-Grunwald-Giemsa stain.
description: One monocyte contained 2 partially aglutinated erythrocytes (lower half of the photo). Another monocyte contained a rounded zone resembling to aglutinated erythrocytes (upper half of the photo). One erythrocytes were detected between and at contact of 2.
description: Imaging mode: microscopy
Visualization methods: x100
DOI: 10.5878/z9az-ga88
title: Hörsel- och bullerdata från svenska förskolor
title: Hearing and Noise Data from Swedish Preschools
title: pDPOAE and Noise Exposure in Swedish Preschools

description: This dataset contains anonymised measurements of hearing function and noise exposure among children in Swedish preschools. Data collection involved two types of objective measurements:

Pressurised Distortion Product Otoacoustic Emissions (pDPOAE) as an indicator of cochlear function.

Noise exposure measurements using personal dosimeters that recorded A-weighted sound levels during preschool attendance.

No tympanometry data are included in the dataset, but tympanometric screening was used as a quality control criterion for inclusion of DPOAE results.

The dataset includes the following variables:

Anonymised participant ID

Measurement timing (morning/afternoon, beginning/end of the week)

LAeqTi (equivalent continuous A-weighted sound level over the measurement interval)

LAFmax95 (95th percentile of maximum A-weighted sound pressure level)

DPOAE amplitudes for the right and left ears at 3–8 kHz, quality controlled according to predefined criteria

Variable definitions, units, and coding schemes are provided in the accompanying codebook.
description: Detta datamaterial innehåller anonymiserade mätningar av hörselfunktion och ljudnivåexponering hos barn i svenska förskolor. Datainsamlingen omfattade två typer av objektiva mätningar:

Pressurised Distortion Product Otoacoustic Emissions (pDPOAE) som indikator på cochleär funktion.

Ljudnivåmätningar med hjälp av personburna dosimetrar som registrerade A-vägda ljudnivåer under förskolevistelsen.

Ingen tympanometridata ingår i datasetet, men tympanometrisk screening användes som kvalitetskriterium för inkludering av DPOAE-resultat.

Datasetet innehåller följande variabler:

Anonymiserat ID

Tidpunkt för mätning (morgon/eftermiddag, början/slut av vecka)

LAeqTi (ekvivalent kontinuerlig ljudnivå under mätperioden)

LAFmax95 (95:e percentilen av maximal A-vägd ljudnivå)

DPOAE-amplituder i höger och vänster öra vid 3–8 kHz, kvalitetssäkrade enligt fördefinierade kriterier

 Variablernas definitioner, enheter och kodningar framgår i den bifogade variabelförteckningen
DOI: 10.6075/j0m61jgc
title: CIL:12918, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 21,000 X. See Ladinsky and Howell (2007) for more information on methods used.
description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation