cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14275374.v1
title: Information items to improve Integration Readiness Levels evaluation

description: Abstract Systems integration is critical to the engineering of successful complex products and systems. The Technology Readiness Levels (TRL) scale assists in making decisions about the infusion of new technologies into complex systems. This scale presents challenges to represent the integration between technologies in a system. The Integration Readiness Levels (IRL) scale aims to address these challenges. A good practice for objective technology readiness assessments is to rely on evidence-based documentation. The TRL scale shows the evidence required for each level of the scale and the appropriate types of documents to collect this evidence, while the IRL scale proposes only the necessary evidence for each level. This research proposes a set of information items to document the required evidence for each level of the IRL scale. The results show the main stages of investigation and the successful tests of the proposal in a case study with spacecraft developed in the binational program China-Brazil Earth Resources Satellite (CBERS). This article contributes to the research in integration readiness assessments, aims to support professionals who carry out evidence-based evaluations and, consequently, may contribute to improving the accuracy of decision-making in the systems integration by using this scale.
DOI: 10.6084/m9.figshare.14275382
title: Characterization of noise emitted by a low-profile tractor and its influence on the health of rural workers

description: Abstract Noise is one of the main physical agents in the rural work environment and it can be harmful to the health of workers exposed to it. Thus, research on the spatialization of noise emitted by agricultural machinery can help minimize harmful health effects. This study sets out to evaluate the spatial distribution of the noise emitted by an agricultural tractor with and without the activation of a rotary cutter. A wave model geostatistical analysis was used to identify healthy working zones according to current legislation. The experiment was carried out using a 75 hp tractor at 2000 rpm with the rotary cutter on the on and off modes. A digital sound level meter was used to register noise in a regular 2.0 x 2.0 m sample mesh within a 10-meter radius. The semivariogram was adjusted using the weighted least squares method, wave model and kriging interpolation to obtain the maps. The magnitude and spatial structure of the noise emitted by the tractor were identified. The results show that the equipment produced noise levels above the limits recommended by Brazilian regulatory standard NR 15. Thus, both the machine operator and the workers involved in the operation should use Personal Protective Equipment.
DOI: 10.6084/m9.figshare.14275383
title: Complexity engineering: New ideas for engineering design and engineering education

description: Abstract This paper presents a theoretical approach to complexity engineering by considering the complex thinking framework proposed by Edgar Morin. The main foundations of this approach are an open system design, emergence, randomness inclusion, and Gödel incompleteness, which are contextualized using real-word instructive problems. Considering these concepts, several conjectures related to engineering activity and engineering education are presented taking several Brazilian catastrophes as counterexamples.
DOI: 10.6084/m9.figshare.14275383.v1
title: Complexity engineering: New ideas for engineering design and engineering education

description: Abstract This paper presents a theoretical approach to complexity engineering by considering the complex thinking framework proposed by Edgar Morin. The main foundations of this approach are an open system design, emergence, randomness inclusion, and Gödel incompleteness, which are contextualized using real-word instructive problems. Considering these concepts, several conjectures related to engineering activity and engineering education are presented taking several Brazilian catastrophes as counterexamples.
DOI: 10.6084/m9.figshare.14275377
title: Assessment of Drug Flow Rate in Skin Cancer Therapy for Enhancing the Drug Delivery System

description: Abstract The major impact in the clinical field is the harm posed by cancer. One most common type of cancer occurs in the skin. Though the conventionally existing modalities are successful in some cases, there is a need for new sensible methods to detect tumors at their initial stage. In accordance to these reasons and in addition to the incapability of the drugs to cross cellular barriers in skin the conventional administration methods are often compromised. To eradicate these problems the research work aims to develop the electrical analogue of skin involving layers like dermis, subcutaneous tissues, bones and muscular layers. The mathematical model has been developed to determine the electrical network of skin. The response of different skin layers are analyzed through simulation studies. It is observed that the cells present in each layer absorbs some amount of drug and let out the remaining to the neighboring layers. Further to minimize the diffusion rate of the drug a conventional controller has been incorporated and the results are analyzed by the contrast of the absorption and diffusion capacities for different layers of skin.
DOI: 10.6084/m9.figshare.14275382.v1
title: Characterization of noise emitted by a low-profile tractor and its influence on the health of rural workers

description: Abstract Noise is one of the main physical agents in the rural work environment and it can be harmful to the health of workers exposed to it. Thus, research on the spatialization of noise emitted by agricultural machinery can help minimize harmful health effects. This study sets out to evaluate the spatial distribution of the noise emitted by an agricultural tractor with and without the activation of a rotary cutter. A wave model geostatistical analysis was used to identify healthy working zones according to current legislation. The experiment was carried out using a 75 hp tractor at 2000 rpm with the rotary cutter on the on and off modes. A digital sound level meter was used to register noise in a regular 2.0 x 2.0 m sample mesh within a 10-meter radius. The semivariogram was adjusted using the weighted least squares method, wave model and kriging interpolation to obtain the maps. The magnitude and spatial structure of the noise emitted by the tractor were identified. The results show that the equipment produced noise levels above the limits recommended by Brazilian regulatory standard NR 15. Thus, both the machine operator and the workers involved in the operation should use Personal Protective Equipment.
DOI: 10.6084/m9.figshare.14275385
title: PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil

description: Abstract Pathogenic microbial detection and control in laboratory animal facilities is essential to guarantee animal welfare, data validity and reproducibility. Helicobacter spp. are known to affect mice health, what may interfere with experimental outcomes. This study aimed to screen for Helicobacter spp. in mice from animal facilities in Rio de Janeiro, Brazil using a PCR-based method. Primers designed to specifically identify Helicobacter spp. were used to amplify feces or intestine DNA extracted of mice from four different animal facilities. The expected 375 base pairs (bp) amplicon was purified, sequenced and a similarity of 95% was observed when compared to deposited sequences of H. hepaticus and H. bilis. In our screening, Helicobacter spp. was detected in ~59% of fecal and ~70% of intestine samples. Our study is the first to screen for Helicobacter spp. in mouse facilities of a Rio de Janeiro University using a low cost, rapid molecular diagnostic test. Although Helicobacter spp. screening is not mandatory according to Brazilian animal welfare regulation it is recommended by institutional animal health monitoring programs guidelines worldwide, including ARRIVE, AAALAC and FELASA.
DOI: 10.6084/m9.figshare.14275385.v1
title: PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil

description: Abstract Pathogenic microbial detection and control in laboratory animal facilities is essential to guarantee animal welfare, data validity and reproducibility. Helicobacter spp. are known to affect mice health, what may interfere with experimental outcomes. This study aimed to screen for Helicobacter spp. in mice from animal facilities in Rio de Janeiro, Brazil using a PCR-based method. Primers designed to specifically identify Helicobacter spp. were used to amplify feces or intestine DNA extracted of mice from four different animal facilities. The expected 375 base pairs (bp) amplicon was purified, sequenced and a similarity of 95% was observed when compared to deposited sequences of H. hepaticus and H. bilis. In our screening, Helicobacter spp. was detected in ~59% of fecal and ~70% of intestine samples. Our study is the first to screen for Helicobacter spp. in mouse facilities of a Rio de Janeiro University using a low cost, rapid molecular diagnostic test. Although Helicobacter spp. screening is not mandatory according to Brazilian animal welfare regulation it is recommended by institutional animal health monitoring programs guidelines worldwide, including ARRIVE, AAALAC and FELASA.
DOI: 10.6084/m9.figshare.14275388
title: Proposal of a theoretical model to identify organizational decline

description: Abstract This study aimed to propose a conceptual model of Identification of Organizational Decline, based on two indicators: Organizational Satisfaction Indicator and Financial Indicator. For the study, we used two bibliometric reviews, which, ultimately, associated the variables of the foundations of excellence in quality with organizational decline. As a result, we observed that the topic of organizational decline in companies has factors of managerial, physical, financial, and behavioral nature, and that their performances worsen by their lack of initiative in using the three planning processes (strategic planning, budget, and control) in a structured way. Regarding companies that seek excellence in quality, these concerns become increasing demands for their managers, who must develop responsible practices and tools to achieve a good performance, bearing in mind the constant search for satisfaction and retention of customers not only for the final product, but throughout the chain process. Thus, the literature on this topic is vast, and this study is justified because it notes a strong relationship between the lack of factors of excellence in management and organizational decline.
DOI: 10.6084/m9.figshare.14275377.v1
title: Assessment of Drug Flow Rate in Skin Cancer Therapy for Enhancing the Drug Delivery System

description: Abstract The major impact in the clinical field is the harm posed by cancer. One most common type of cancer occurs in the skin. Though the conventionally existing modalities are successful in some cases, there is a need for new sensible methods to detect tumors at their initial stage. In accordance to these reasons and in addition to the incapability of the drugs to cross cellular barriers in skin the conventional administration methods are often compromised. To eradicate these problems the research work aims to develop the electrical analogue of skin involving layers like dermis, subcutaneous tissues, bones and muscular layers. The mathematical model has been developed to determine the electrical network of skin. The response of different skin layers are analyzed through simulation studies. It is observed that the cells present in each layer absorbs some amount of drug and let out the remaining to the neighboring layers. Further to minimize the diffusion rate of the drug a conventional controller has been incorporated and the results are analyzed by the contrast of the absorption and diffusion capacities for different layers of skin.
DOI: 10.6084/m9.figshare.14275386
title: Maternal and developmental toxicity after exposure to formulation of chlorothalonil and thiophanate-methyl during organogenesis in rats

description: Abstract Chlorothalonil and thiophanate-methyl are fungicides widely used in agriculture. The aim of this study was to assess maternal toxicity and embryotoxic potential of exposure to chlorothalonil and thiophanate-methyl during organogenesis period in rats. Pregnant rats were divided into four groups: control and exposed to 400 (CT400), 800 (CT800) and 1200 mg-1kg bw-1 day (CT1200) of commercial formulation constituted of 200 g of thiophanate-methyl kg−1 and 500 g of chlorothalonil kg−1 by gavage, from 6th to 15th gestational day. Maternal toxicity, liver, kidney and placenta histology, reproductive performance, and external, skeletal and visceral malformations of fetuses were evaluated. Maternal liver weight was decreased in CT1200 group and focal necrosis and microvesicular steatosis, inflammatory infiltrate and hepatocytes with pyknotic nucleus were observed in CT800 and CT1200 groups. Reproductive performance was similar among groups. The percentage of fetuses small for pregnancy age was increase in CT400 and CT800 groups. Moreover, incidence of skeletal anomalies was increased in the three groups exposed to fungicides. Chlorothalonil and thiophanate-methyl exposure showed affect the prenatal development and induce maternal toxicity.
DOI: 10.6084/m9.figshare.14275386.v1
title: Maternal and developmental toxicity after exposure to formulation of chlorothalonil and thiophanate-methyl during organogenesis in rats

description: Abstract Chlorothalonil and thiophanate-methyl are fungicides widely used in agriculture. The aim of this study was to assess maternal toxicity and embryotoxic potential of exposure to chlorothalonil and thiophanate-methyl during organogenesis period in rats. Pregnant rats were divided into four groups: control and exposed to 400 (CT400), 800 (CT800) and 1200 mg-1kg bw-1 day (CT1200) of commercial formulation constituted of 200 g of thiophanate-methyl kg−1 and 500 g of chlorothalonil kg−1 by gavage, from 6th to 15th gestational day. Maternal toxicity, liver, kidney and placenta histology, reproductive performance, and external, skeletal and visceral malformations of fetuses were evaluated. Maternal liver weight was decreased in CT1200 group and focal necrosis and microvesicular steatosis, inflammatory infiltrate and hepatocytes with pyknotic nucleus were observed in CT800 and CT1200 groups. Reproductive performance was similar among groups. The percentage of fetuses small for pregnancy age was increase in CT400 and CT800 groups. Moreover, incidence of skeletal anomalies was increased in the three groups exposed to fungicides. Chlorothalonil and thiophanate-methyl exposure showed affect the prenatal development and induce maternal toxicity.
DOI: 10.6084/m9.figshare.14275388.v1
title: Proposal of a theoretical model to identify organizational decline

description: Abstract This study aimed to propose a conceptual model of Identification of Organizational Decline, based on two indicators: Organizational Satisfaction Indicator and Financial Indicator. For the study, we used two bibliometric reviews, which, ultimately, associated the variables of the foundations of excellence in quality with organizational decline. As a result, we observed that the topic of organizational decline in companies has factors of managerial, physical, financial, and behavioral nature, and that their performances worsen by their lack of initiative in using the three planning processes (strategic planning, budget, and control) in a structured way. Regarding companies that seek excellence in quality, these concerns become increasing demands for their managers, who must develop responsible practices and tools to achieve a good performance, bearing in mind the constant search for satisfaction and retention of customers not only for the final product, but throughout the chain process. Thus, the literature on this topic is vast, and this study is justified because it notes a strong relationship between the lack of factors of excellence in management and organizational decline.
DOI: 10.6084/m9.figshare.14275389
title: Air entrainment and pressure drop in low-cost ejectors

description: Abstract This study investigated experimentally the air entrainment and pressure drop in low-cost ejectors composed of two pieces shaped from PVC bars inserted in a 25 mm T-junction of the same material. The hydraulic behavior was very similar for the different ejector designs, and linear relationships between the water and air flow rates were fitted. However, when a rotameter was installed at the air line, the head losses resulted in a pronounced decrease (3-fold) in the air entrainment rate. The maximum air-water entrainment ratios reached by the low-cost ejectors was 1.7, while the pressure drop was about 80% of the upstream pressure. The results suggest that these ejectors have a better benefit-cost ratio than conventional ones for applications such as aeration and mixing in reactors, tanks and water bodies. Comparing our results with those obtained previously by using water both as primary and suction fluids, it was shown that under gas-liquid flow conditions the entrainment ratio was about 2.5 times larger than that for the single-phase case, while the pressure drop was about 15% higher. This was attributed to the lower density of the air and the higher dissipation of turbulent kinetic energy due to bubble-liquid interactions in the two-phase flow case.
DOI: 10.6084/m9.figshare.14275389.v1
title: Air entrainment and pressure drop in low-cost ejectors

description: Abstract This study investigated experimentally the air entrainment and pressure drop in low-cost ejectors composed of two pieces shaped from PVC bars inserted in a 25 mm T-junction of the same material. The hydraulic behavior was very similar for the different ejector designs, and linear relationships between the water and air flow rates were fitted. However, when a rotameter was installed at the air line, the head losses resulted in a pronounced decrease (3-fold) in the air entrainment rate. The maximum air-water entrainment ratios reached by the low-cost ejectors was 1.7, while the pressure drop was about 80% of the upstream pressure. The results suggest that these ejectors have a better benefit-cost ratio than conventional ones for applications such as aeration and mixing in reactors, tanks and water bodies. Comparing our results with those obtained previously by using water both as primary and suction fluids, it was shown that under gas-liquid flow conditions the entrainment ratio was about 2.5 times larger than that for the single-phase case, while the pressure drop was about 15% higher. This was attributed to the lower density of the air and the higher dissipation of turbulent kinetic energy due to bubble-liquid interactions in the two-phase flow case.
DOI: 10.6084/m9.figshare.14275391.v1
title: Epigallocatechin gallate-capped gold nanoparticles enhanced the tumor suppressors let-7a and miR-34a in hepatocellular carcinoma cells

description: Abstract Epigallocatechin gallate (EGCG), major constituent of green tea, possesses antioxidant, antiviral, and anticancer activities. Gold nanoparticles (AuNPs) play an important role in drug delivery due to their stability, ease of surface functionalization, and unique optical properties. This study aimed to investigate the influence of EGCG-capped AuNPs on tumor suppressor miRNAs (miR-34a and let-7a) and their targeted cell death mediators in HepG2 cells, compared with celastrol. EGCG-AuNPs were prepared and characterized. antioxidant activity was estimated by DPPH scavenging assay; cytotoxicity was assessed by MTT assay; let-7a and miR-34a expression was analyzed by qPCR; and miRNAs targets (c-Myc and caspase-3) were assessed by ELISA and immunocytofluorescence, respectively. The average size of EGCG-AuNPs was 35 nm, with a λmax of ~535 nm. EGCG-AuNPs exerted cytotoxicity on HepG2 cells stronger than that of EGCG alone. EGCG-AuNPs and EGCG presented half-maximal radical scavenging concentrations (SC50) of 539 µg/ml and 45 µg/ml, respectively. The expression levels of let-7a and miR-34a were significantly elevated in HepG2 cells after EGCG-AuNP treatment for 72 h. c-Myc protein expression was reduced, whereas caspase-3 expression was increased following treatment with EGCG-AuNPs. In conclusion, Au-NPs are effective carrier for EGCG, and EGCG-AuNPs are promising anti-cancer agent.
DOI: 10.6084/m9.figshare.14275391
title: Epigallocatechin gallate-capped gold nanoparticles enhanced the tumor suppressors let-7a and miR-34a in hepatocellular carcinoma cells

description: Abstract Epigallocatechin gallate (EGCG), major constituent of green tea, possesses antioxidant, antiviral, and anticancer activities. Gold nanoparticles (AuNPs) play an important role in drug delivery due to their stability, ease of surface functionalization, and unique optical properties. This study aimed to investigate the influence of EGCG-capped AuNPs on tumor suppressor miRNAs (miR-34a and let-7a) and their targeted cell death mediators in HepG2 cells, compared with celastrol. EGCG-AuNPs were prepared and characterized. antioxidant activity was estimated by DPPH scavenging assay; cytotoxicity was assessed by MTT assay; let-7a and miR-34a expression was analyzed by qPCR; and miRNAs targets (c-Myc and caspase-3) were assessed by ELISA and immunocytofluorescence, respectively. The average size of EGCG-AuNPs was 35 nm, with a λmax of ~535 nm. EGCG-AuNPs exerted cytotoxicity on HepG2 cells stronger than that of EGCG alone. EGCG-AuNPs and EGCG presented half-maximal radical scavenging concentrations (SC50) of 539 µg/ml and 45 µg/ml, respectively. The expression levels of let-7a and miR-34a were significantly elevated in HepG2 cells after EGCG-AuNP treatment for 72 h. c-Myc protein expression was reduced, whereas caspase-3 expression was increased following treatment with EGCG-AuNPs. In conclusion, Au-NPs are effective carrier for EGCG, and EGCG-AuNPs are promising anti-cancer agent.
DOI: 10.6084/m9.figshare.14275392
title: Volatile Compounds of Different Fresh Wet Noodle Cultivars Evaluated by Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry

description: Abstract This study was carried out to determine the volatile compounds from four samples of fresh wet noodles and the changes in the volatile compound composition during the storage process. The volatile compounds from four samples of fresh wet noodles were characterized by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS). The compositions of the volatile compounds varied among fresh and cooked wet noodles made from the raw potato/wheat flour or wheat flour. A total of 194 volatile compounds were detected in the raw potato noodles, main volatiles including aldehydes, alcohols, ketones, esters and organic acids. The total volatile compounds in the potato/wheat flour noodle samples contained mainly aldehyde compounds and were greater than those in the wheat noodles. The total volatile compounds in the cooked noodle samples were greater than those in raw noodle samples. Alcohols and ketones were the least common types of volatile substances in the samples at 0 h. During storage time, alcohols and ketones were increased in volatile substances, and the amount of acids increased dramatically. The results indicated that the aroma of fresh wet noodles was affected by the storage process.