cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3886/e241385
title: Archbishop Charles Heerey College of Nursing Sciences, Aba, Abia State, 2026/2027 Admission Form/Application Form Is Out Call the Registrar (08058556110) 08058556110 (08058556110) For More Details On How To Apply And Register Online.

description: Archbishop Charles Heerey College of Nursing Sciences, Aba, Abia State, 2026/2027 Admission Form/Application Form Is Out Call the Registrar (08058556110) 08058556110 (08058556110) For More Details On How To Apply And Register Online. The Management Of The School Hereby Inform The general Public On The Sales Of The General Nursing Admission Form Into The School Of Nursing, And Also The Sales Of Two (2) Years Post Basic Nursing Program. Contact The Educational Board Admin On (08058556110) For More Details On How To Purchase The Form And Register Online. REQUIREMENT FOR THE THREE YEARS GENERAL NURSING PROGRAMMES1. GENERAL NURSING PROGRAMME Admission Requirements Candidates For Admission Into The General Nursing Program Of The Nursing And Midwifery Council Of Nigeria Must Posses The Senior Secondary School Certificate Or It’s Equivalent With Five Credits To Include English Language And Either Physics, Chemistry, Biology/Health Science At Not More Than Two sittings. Duration Of Training: Three Calendar Years. Registered Nurse (RN) Also POST BASIC NURSING MIDWIFERY PROGRAMME Admission Requirements: The Candidate Must Be a Registered Nurse, Five (O,Level) Credits Not More Two Sittings And Must Also Meet All institutional Requirements. Duration of Training: Two Calendar Year.{08058556110}
DOI: 10.3535/d5f-467-0ss
title: Thlaspi arvense L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-86l-xld
title: Scolochloa festucacea (Willd.) Link

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-988-z59
title: Carex elongata L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-9wb-1cm
title: Alnus glutinosa f. obcordata

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-cne-29x
title: Chelidonium majus L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5e-pxy-zez
title: Trifolium medium L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-h2p-659
title: Potamogeton alpinus Balb.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-k9c-0jd
title: Anthoxanthum australe (Schrad.) Veldkamp

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-qds-skt
title: Carex leporina L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-x56-d9j
title: Cerastium arvense L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-1l4-bv1
title: Spinulum annotinum (L.) A. Haines

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-27p-ee5
title: Sagina procumbens L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-2gl-f2z
title: Rumex acetosa L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-57y-l4v
title: Alopecurus aequalis Sobol.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-das-wxh
title: Alisma plantago-aquatica L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-jfp-4at
title: Ribes spicatum subsp. spicatum

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-kf6-zl4
title: Juncus bulbosus L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-kkc-6rr
title: Briza minor L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-qhn-mjt
title: Carex L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-s9a-xem
title: Convallaria majalis L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5f-xpz-dwz
title: Bolboschoenus maritimus (L.) Palla

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5g-xcj-6cp
title: Gagea minima (L.) Ker Gawl.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5h-816-ew0
title: Potentilla intermedia L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5h-9vh-ahf
title: Silene acaulis (L.) Jacq.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5h-e3r-xfp
title: Rorippa × armoracioides (Tausch) Fuss

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5h-hhg-yn0
title: Calamagrostis canescens (Weber) Roth

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5h-v97-fb9
title: Festuca ovina L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5j-3ce-aqw
title: Luzula pallescens Sw.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5j-4ak-bqn
title: Carex spicata Huds.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5j-6zg-79m
title: Dryopteris expansa (C. Presl) Fraser-Jenk. & Jermy

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5j-w5v-snc
title: Eleocharis acicularis (L.) Roem. & Schult.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5j-zfj-8ht
title: Eriophorum angustifolium Honck.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-025-tab
title: Thalictrum flavum L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-142-b6g
title: Ranunculus acris subsp. acris

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-4rl-hd0
title: Aegilops cylindrica Host

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-cpc-297
title: Salix myrsinites L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-j7x-cbh
title: Epipactis helleborine (L.) Crantz

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-l5p-1yj
title: Vicia sylvatica L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-m56-xsv
title: Alisma plantago-aquatica L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-n67-c3r
title: Fumaria officinalis L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-qbm-5nl
title: Actaea spicata L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-rtc-cvk
title: Coronilla varia L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5k-207-tmq
title: Agrimonia eupatoria L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5l-5xz-xsc
title: Juncus L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5l-9es-fns
title: Eleocharis acicularis (L.) Roem. & Schult.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5l-ns0-w87
title: Persicaria minor (Huds.) Opiz

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5l-rwb-5hc
title: Reseda lutea L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5l-yr0-rjy
title: Ribes nigrum L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5l-z56-hbl
title: Chelidonium majus L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-18y-3z7
title: Stellaria graminea L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-2w8-xlc
title: Spinulum annotinum subsp. alpestre (Hartm.) Uotila

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-a9b-sgl
title: Eleocharis palustris subsp. palustris

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-bfp-a21
title: Carex arenaria L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-ch8-zhs
title: Poa glauca Vahl

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-gth-s6b
title: Carex acuta L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-m9t-xax
title: Plantae

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5m-rvd-agf
title: Dactylorhiza maculata (L.) Soó

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5n-124-cct
title: Persicaria amphibia (L.) Gray

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5n-d67-jt0
title: Lathyrus linifolius (Reichard) Bässler

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5n-de0-b7g
title: Malaxis monophyllos (L.) Sw.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5n-h2f-vyr
title: Zannichellia P.Micheli ex L.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d5n-lsv-eq9
title: Potentilla anglica Laich.

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.29309696
title: Figure 4C

description: <b>A.</b> Lentivirus-mediated downregulation of MyD88. MRC-5 cells were transduced with control lentivirus or lentivirus encoding shRNA targeting MyD88 (shMyD88-1 and shMyD88-2) and selected using puromycin. Knockdown efficiency was assessed by western blot analysis. Blots were probed for MyD88 and p115 as loading control. <b>B. </b>The transduced and selected MRC-5 cells were infected with TB40/E-mCh virus at MOI of 0.05 and spread of the virus was monitored by confocal imaging. Representative images from day 3, 9 and 15 are shown. <b>C. </b>The spread of infection was quantified as the area of fluorescence using the NIS element software. <b>D. </b>MRC-5 cells were transduced with lentivirus from control or two different cassettes encoding the MyD88 gene (MyD88-1 and MyD88-2) and subjected to puromycin selection. Cell lysates were harvested and run on western blot to analyse MyD88 levels. Tubulin served as a loading control. <b>E. </b>The transduced and selected MRC-5 cells were infected with TB40/E-mCh virus at MOI of 0.05 and the spread of the virus was monitored every three days by confocal imaging up to day 21. Representative images from day 3, 9 and 15 are shown. <b>F. </b>The spread of infection was quantified as the area of fluorescence using the NIS element software.
DOI: 10.6084/m9.figshare.29309696.v1
title: Figure 4C

description: <b>A.</b> Lentivirus-mediated downregulation of MyD88. MRC-5 cells were transduced with control lentivirus or lentivirus encoding shRNA targeting MyD88 (shMyD88-1 and shMyD88-2) and selected using puromycin. Knockdown efficiency was assessed by western blot analysis. Blots were probed for MyD88 and p115 as loading control. <b>B. </b>The transduced and selected MRC-5 cells were infected with TB40/E-mCh virus at MOI of 0.05 and spread of the virus was monitored by confocal imaging. Representative images from day 3, 9 and 15 are shown. <b>C. </b>The spread of infection was quantified as the area of fluorescence using the NIS element software. <b>D. </b>MRC-5 cells were transduced with lentivirus from control or two different cassettes encoding the MyD88 gene (MyD88-1 and MyD88-2) and subjected to puromycin selection. Cell lysates were harvested and run on western blot to analyse MyD88 levels. Tubulin served as a loading control. <b>E. </b>The transduced and selected MRC-5 cells were infected with TB40/E-mCh virus at MOI of 0.05 and the spread of the virus was monitored every three days by confocal imaging up to day 21. Representative images from day 3, 9 and 15 are shown. <b>F. </b>The spread of infection was quantified as the area of fluorescence using the NIS element software.
DOI: 10.6084/m9.figshare.29307374
title: Figure 3B

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.6084/m9.figshare.29307374.v1
title: Figure 3B

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.15468/dl.6vumxk
title: Occurrence Download

description: A dataset containing 539 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Türkiye",
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Pinus brutia Ten., Pinus halepensis Mill.)"
 ]
}

 The dataset includes 539 records from 41 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044512-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29307395
title: Figure 3C

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.6084/m9.figshare.29307395.v1
title: Figure 3C

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.6084/m9.figshare.29307659
title: Figure 3D

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.6084/m9.figshare.29307659.v1
title: Figure 3D

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.15468/dl.8afp9j
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Crassula gemmifera Friedrich",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((20.7576 -24.3688,20.529 -24.0715,20.509 -24.0715,20.509 -28.5786,25.5395 -28.5786,25.5395 -24.0715,25.1306 -24.0715,24.9333 -24.4571,23.8962 -24.2467,22.8065 -24.295,22.3719 -24.7207,22.2323 -25.547,22.0387 -25.7188,21.7833 -25.7784,21.5388 -25.7113,21.3553 -25.5415,20.7576 -24.3688))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044514-250525065834625/datasets/export for details.
DOI: 10.6084/m9.figshare.29307236
title: Figure 3A

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.
DOI: 10.6084/m9.figshare.29307236.v1
title: Figure 3A

description: <b>A. </b>MRC-5 cells expressing either vector or UL88 were treated with DMSO, MG132 (10 mM) or Chloroquine (50 mM). Lysates were analysed by western blotting. Blots were probed for UL88, MyD88 and p115 (loading control). Representative blots are shown. <b>B. </b>Graph shows quantitation from three independent experiments as described in <b>A</b>.<b> C. </b>293 cells were co-transfected with HA-MyD88 and either GFP vector, GFP-UL88 full length, GFP-UL88 C, or GFP-UL88 N. Lysates were harvested 48 h post-transfection and analysed by western blotting. Blots were probed for HA, GFP and p115 (loading control)<b>. D.</b> 293 cells co-transfected with MyD88-flag and either GFP-UL88 full length, GFP-UL88 C or GFP-UL88 N. Lysates were harvested at 48 h post transfection and subjected to co-immunoprecipitation using magnetic anti-flag beads. Samples were analysed by western blot and probed for GFP, flag and p115 as a loading control. Arrows point to the GFP-UL88, GFP-UL88 N and Flag-MyD88 in the IP blot.