cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14455/tsb.res.2019.27
title: Expression of an Immunogenic Protein derived from Spike Protein of Porcine Epidemic Diarrhea Virus in two E. coli Strains

description: Escherichia coli is recognized as a fast, easy and inexpensive host system for production of valuable proteins including subunit proteins for vaccination. In this study, we studied whether PEDVSME, a new immunogen designed from spike protein of PEDV, can be produced in E. coli by studying in two strains: BL21(DE3) and BL21(DE3)pLysS. The prokaryotic expression plasmid, pET28b, was generated to contain the PEDVSME gene. Recombinant clone of pET28b.PEDVSME was successfully generated and selected in E. coli DH5?. Two strains of E. coli, BL21(DE3) and BL21(DE3)pLysS, were then transformed with recombinant plasmid pET28b.PEDVSME. Expression of the PEDVSME gene was induced with 1 mM IPTG. Analyzed by SDS-PAGE and Western blot, PEDVSME was not detected in E. coli BL21(DE3) but highly expressed in strain BL21(DE3)pLysS. Importantly, although the PEDVSME protein could be observed in soluble form, majority of the protein was found in insoluble form (inclusion body). This work provides useful information regarding appropriate E. coli host strain for efficient production of the immunogen PEDVSME, bringing a feasibility of using PEDVSME as a subunit vaccine.
DOI: 10.14455/tsb.res.2019.28
title: Flowering time regulatory network controlled by FLOWERING LOCUS C (FLC) in response to temperature changes

description: The global temperature has been fluctuating drastically due to global warming. This has major effects on living organisms around the world, especially in sessile organism such as plants. In flowering plants, initiation of flowering is a crucial developmental event, which requires both internal and environmental signals to determine when floral transition should occur, in order to maximize reproductive success. One of the key environmental signals that determine flowering time is ambient temperature. In this study, we aimed to investigate the influence on flowering of daily variable temperature, where temperature fluctuates throughout the day, in selected Arabidopsis accessions with different alleles of the key flowering regulators, FLOWERING LOCUS C (FLC) and FRIGIDA (FRI), namely Col-0, C24 and their late flowering hybrid, C24xCol-0. Our result showed that Col-0, C24 and C24xCol-0 grown in a constant ambient temperature flowered later than those grown in a variable temperature mimicking daily fluctuation in both wild-types and late flowering hybrid. Besides, we have explored the connection between FLC and its up- and downstream regulatory genes, in response to temperature changes, using publicly available data. The transcriptional changes of flowering genes were observed as a result of a temperature shift, which were combined with an FLC- associated flowering time regulatory network to reveal the transcriptional dynamics in temperature shift conditions. The network can be further expanded to cover other flowering genes and thus provide more comprehensive model of expression dynamics of flowering genes in response to temperature changes.
DOI: 10.14455/tsb.res.2019.29
title: Growth inhibition of Klebsiella quasipneumoniae contamination on fresh-cut mango by vapor-phase vinegar and pure acetic acid
DOI: 10.14455/tsb.res.2019.30
title: Gut microbiota alteration along gastrointestinal tract in SLE (systemic lupus erythematosus)

description: The balance of microbiological ecology in the intestine (homeostasis) is essential for health. Dysbiosis of gut microbiota and diversity can lead to metabolic disorders and inappropriate immune responses, hence were reported to be associated with many diseases, including systemic lupus erythematosus (SLE), a chronic autoimmune disease. Because differences of physicochemical conditions in each intestinal section (duodenum, jejunum, ileum, cecum and colon) affect the composition of gut microbiota. This study we firstly reported gut microbiota composition along each section of mouse gastrointestinal tract comparing between lupus-prone mice by genetic FcGRIIb knockout and agedmatched healthy mice, at 4 months ( preclinical SLE) and 11 months (established SLE) of ages, to identify potentially beneficial and harmful bacteria. Gut microbiota were different in bacterial composition and diversity. The cecum and colon had the most significant differences between healthy and disease. In disease group, f_S24-7_unclassified, Anaerostipes, Lactobacillus, Sutterella, Allobaculum, Adlercreutzia and Bifidobacterium, as potential beneficial bacteria, decreased
description: whereas Mucispirillum, Oscillospira, f_Lachnospiraceae_ unclassified, Alistipes, and Sporobacter, as potential harmful bacteria, increased.
DOI: 10.14455/tsb.res.2019.31
title: Identification and Characterization of Defensin-related Genes Challenged with Fungal Pathogen Infection in Cassava

description: Plant defensin family is one of the cysteine-rich antimicrobial peptides, which play important roles in plant immunity for responding against the invasion of pathogens. They are small cationic peptides which are shown to inhibit growth of a broad range of microorganisms. One of the major disease problems on production of cassava (Manihot esculenta Crantz) is cassava anthracnose disease, causing up to 80% yield loss by fungus Collectotrichum gloeosporiodes f. sp. manihotis (CAD). This research aims to identify and characterize defensin-related genes in cassava genome and investigated expression pattern of defensin genes upon CAD pathogen infection and to study the effect of phytohormone pre-treatment prior to fungal infection. The results of bioinformatics analysis showed that the coding sequences of 5 defensin gene members were identified in cassava genome and phylogenetic tree was constructed to verify each gene member. Also, defense-related response motifs were predicted in their promoter regions. Differential expression profiles of MeDef and MeDef1 . 2 were observed in CAD infection and phytohormone pre-treatment of the highly susceptible (HN) and less susceptible/ more resistant (HB60) cassava cultivars. The strong up-regulation of MeDef was found in HB60, whereas late induction of this gene was observed in HN. MeDef1 . 2 was not expressed. Salicylic acid pre-treatment prior to CAD infection could enhance defensive in HB60. This finding suggested that defensin has a potential function in CAD defense response in cassava, and further study should be useful for cassava improvement program to fungal pathogen resistance.
DOI: 10.14455/tsb.res.2019.32
title: Identification of Genes Encoding Annexin in Cassava Genome and Expression Profile of MeAnn1 and MeAnn2 in Response to Pathogen Infection

description: Cassava (Manihot esculenta Crantz. ) is an important economic crop used as a raw material in food and various non-food industries. Nowadays, cassava production was limited by various diseases. Among several diseases, cassava anthracnose disease is one major fungal disease caused by Colletotrichum gloeosporioides f.sp. manihotis (CAD). Interestingly, there is a group of calcium-dependent membrane binding protein "Annexin", which can stimulate plant immunity upon pathogen infections. This study focused on identification and expression profiles of candidate annexin coding genes in cassava upon CAD infection. The bioinformatics study indicated that there were 10 cassava genes encoding annexin in which their promoter regions contained 28 cis-regulatory motifs related to plant immune system such as jasmonic acid ( JA) - responsive gene, salicylic acid ( SA) - responsive gene, resistance gene and others. The phylogenetic tree was constructed to verify each member of annexins and the protein structure was predicted. Disease Severity Rating suggested different resistant ability to CAD infection among the 4 cassava cultivars. The two cassava cultivars, Hanatee (HN, susceptible to CAD) and Huaybong60 (HB60, less susceptible to CAD) were selected to study. The differential expression of candidate genes encoding annexin was observed at different time points after CAD infection and in response to phytohormone (SA and JA) pre-treatment. The result showed possible function of cassava annexins related to defense response in CAD-susceptible and -less susceptible cultivars. This investigation on the role of annexins in relation with disease resistance in cassava will be useful for breeding program to improve cassava to resist against pathogen infection in future.
DOI: 10.14455/tsb.res.2019.33
title: Immobilization of Alternansucrase on Chitosan Beads

description: Alternansucrase (Alt, EC 2.4.1.140) is an enzyme that catalyzes transferring of an ?-D-glucosyl residue from sucrose to another sucrose molecule which acts as an acceptor, thereby creating a glucan with alternating ?-1,6 and ?-1,3-glycosidic linkages, known as alternan. The truncated alt (?Calt) gene was expressed in E. coli BL21(DE3) and recombinant Alt was partially purified by DEAE anion exchange chromatography. The recombinant Alt possessed an optimum temperature and pH of 40?C and 4.0, respectively. In order to reuse the enzyme and to enhance its stability, Alt was immobilized on a chitosan support by covalent method using glutaraldehyde as a crosslinking agent. Chitosan beads were activated under optimal immobilization condition which was 0.5% (w/v) glutaraldehyde in 50 mM citrate buffer pH 6.0 at 4?C overnight. After that, the activated chitosan beads were incubated with an enzyme solution (30 U or 0.69 mg/g of beads) at 4?C overnight. The immobilized enzyme will be further employed for the production of alternan.
DOI: 10.14455/tsb.res.2019.34
title: In Situ Increased Reducing Sugar of Nang Lae Pineapple Juice

description: Nang Lae pineapple, (Ananas comosus (L) Merr.), has planted in Chiang Rai Province, could be used to prepare juice and achieved 35.5?2.0 mL per fresh fruit (100 g). The reducing sugar of six pineapple juice samples were determined using DNS method, which contained 42.22?14.23 mg/mL. Baker's yeast (5% w/v) was immobilized by entrapment method using 0.5% ( w/ v) sodium alginate. Yeast alginate entrapment bead was applied to pineapple juice and it could increase reducing sugar three times within 24 h by sucrose hydrolyzed enzyme. Activity of extracellular enzyme produced in 50 mM calcium chloride at 30 oC established 0.32 U/mL. Application of 5 and 10% (v/v) extracellular enzyme in pineapple juices could increase high content of reducing sugar within 20 min.
DOI: 10.14455/tsb.res.2019.35
title: Influence of Different Gene Integration Strategies on Transcriptional Silencing at rDNA Gene in Saccharomyces cerevisiae

description: Chromatin silencing at ribosomal DNA (rDNA silencing) is the crucial phenomenon for prevention of homologous recombination of repetitive rDNA sequences. In the previous study, rDNA silencing was shown to play a role in preservation of stability of rDNA (RDN1) locus contributing to enhance yeast lifespan. The silencing efficiency of rDNA locus can be detected by integration of reporter gene into rDNA locus. However, the proper strategy for integration of a reporter gene into rDNA locus has been controversial. This study showed that different type of gene integration strategies, gene insertion and gene replacement, affect silencing capability at rDNA locus of yeast Saccharomyces cerevisiae AS14. A reporter gene, GIN11M86 growthinhibitory sequence under the control of galactose-inducible promoter, was inserted into external transcribed spacer1 (ETS1) gene and replaced into ETS118S (ETS1 RDN18) gene of rDNA locus. The results showed that ETS1 RDN18- replaced strain exhibited a reduced growth rate significantly in comparison with that of the ETS1-inserted and wild-type strain grown under galactose- induced condition. This suggested that gene insertion is the appropriate integration strategy for assessment of rDNA silencing together with study of the critical factor to silencing at rDNA locus.
DOI: 10.14455/tsb.res.2019.36
title: Inhibition of Hydrogen Synthesis Increased Accumulations of Pigments in Cyanobacterium Synechocystis sp. PCC 6803

description: Cyanobacterium Synechocystis sp. PCC 6803 produces one hydrogen gas molecule from two NADPH molecules via the only catalysis of the enzyme [NiFe]-hydrogenase composing of several subunits: HoxE, HoxF, HoxU, HoxY, and HoxH. Effects of the inhibition of hydrogen synthesis on the accumulation levels of other bioproducts have yet to be determined. Here, the two main catalytic subunits of [NiFe]-hydrogenase (HoxY and HoxH) were inactivated by the deletion of both hoxY and hoxH gene. Inactivation of both hoxY and hoxH gene did not have a deleterious effect to cell growth, suggesting that the hydrogen synthesis is not an essential process for cell viability. In addition, inactivation of both hoxY and hoxH genes significantly increased the accumulation levels of phycocyanin by 1.55 fold, and allophycocyanin by 1.49 fold. The biosynthesis of hydrogen (via hydrogenase), phycocyanin and allophycocyanin require the same substrate - NADPH. Thus, the inhibition of the hydrogen synthesis (via hoxY and hoxH inactivation) might help increase cellular NADPH level and mediate the enhanced production of phycocyanin and allophycocyanin.
DOI: 10.14455/tsb.res.2019.37
title: Invertase Production from Saccharomyces cerevisiae DB-KKU-Y-53 Using Low Cost Medium

description: Invertase (?-fructofuranosidase
description: EC 3.2.1.26) catalyzes the hydrolysis of sucrose into glucose and fructose. Since these invert sugars have desirable properties such as a source of instant energy, high osmotic pressure and solubility, they are widely used in food, pharmaceutical, cosmetic, medicine and beverage industries. Saccharomyces cerevisiae, a non-pathogenic microorganism, is easily to cultivate and handle during enzyme production. This yeast strain is normally used as the main source of invertase for food industry. In this study, production of invertase from S. cerevisiae DB-KKUY53 using low cost medium has been investigated. To achieve the optimal composition of medium for invertase production, response surface methodology was employed. Screening of the significant variables for enzyme production was conducted using Plackett-Burman Design. The results suggested that concentrations of ammonium sulfate, magnesium sulfate and sucrose were found to be the statistically significant variables for invertase production (p &lt; 0.05). Then, the optimal composition of medium was studied through central composite design. The results indicated that the specific activity of invertase increased to 56.70% (5.14 U/mg) when this yeast was cultivated with the medium containing ammonium sulfate concentration of 3.5 g/l, magnesium sulfate concentration of 1.0 g/l and sucrose concentration of 13.2 g/l, respectively. Furthermore, statistical analysis implied that the mathematical model was statistically significant (p &lt; 0.01). The predicted enzyme activities were in agreement with the experimental results and the difference was less than 10%.
DOI: 10.14455/tsb.res.2019.38
title: Investigation of Cassava Protein-protein Interaction Alterations: Cellular Response to Cassava Brown Streak Virus Infection

description: Cassava (Manihot esculenta Crantz) is an important global crop commonly grown in Africa and South East Asia, including Thailand. The valuable starch extracted from cassava storage roots is used for many industrial purposes, resulting in increased demand for starch-enriched storage roots. However, cassava production faces the threat of pathogens, one of which is the cassava brown streak virus (CBSV), which can lead to substantial yield and quality losses. Unfortunately, the knowledge of cassava defense mechanism against CBSV is limited
description: thus, systems biology approach was applied in this work to investigate cassava responses during viral infection, by integrating cassava gene expression data into the protein-protein interaction (PPI) network. Changes in the cassava protein interactome in control (0 DAG), and at early (5 DAG), and late infection (45 DAG) were studied. A total of 1,702,892 interactions among 16,506 proteins were identified in control, and the amount increased to 2,454,592 interactions (19,905 proteins) at early viral infection, before decreasing to 1,321,954 (14,726 proteins) at late infection. The protein interactome subnetworks relating to disease resistance proteins and NAC transcription factors were used to demonstrate plant protein interactome response to viral infection. This study would enable us to comprehend plant responses to viral pathogens and shed light on the efficient development of early warning systems to protect plants from viral diseases in the future.
DOI: 10.14455/tsb.res.2019.39
title: Isolation and Characterisation of Actinomycetes from Mangrove forest and Hot spring Soils, Thailand

description: Fifteen isolates of actinomycetes from the mangrove forest Samet, Mueang, Chonburi and twenty four isolates from Suan Phueng hot spring, Ratchaburi were isolated, Morphology and biochemical tests were examined. Antimicrobial activity against 6 tested microorganisms
description: Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Kocuria rhizophila ATCC 9341, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231 found 6 isolates which have inhibition zone on ISP2 agar by preliminary test technique. Agar disc diffusion was performed on Glucose Yeast extract agar (GYE). The strains showed greater than 99% 16S rRNA gene sequence similarity to the type strains of several recognized species of the genus Streptomyces, but in the phylogenetic tree based on 16S rRNA gene sequences of 3 isolates, it formed a distinct phyletic line and demonstrated closest relationships to Streptomyces griseoincarnatus LMG 19316T (AJ781321), Streptomyces sundarbansensis MS1/7T (AY550275) and Streptomyces griseorubens NBRC 12780T (AB184139) with similarity percentage of 98.64, 99.79 and 99.72, respectively.
DOI: 10.14455/tsb.res.2019.40
title: Isolation of Bacterial Cellulose Nanofibers Producing Acetic Acid Bacteria from Fermented juices

description: Bacterial cellulose (BC) are high purity, ultra-fine network of nanofibrils structure, and non-toxic. The nanofibrils bacterial cellulose used for many applications in the food, medical and cosmetics industries. The objectives of this study aim to isolate and identify of nanofibrils bacterial cellulose producing acetic acid bacteria (AAB). We studied, isolation of cellulose producing AAB from 26 samples of Kombucha culture and different kinds of fermented fruit, and herb juices on Histrin Schramm. One hundred and seventeen bacterial isolates were obtained. The isolates were tested for their biochemical characteristics and bacteria cellulose nanofibrils production. Four isolates: RC1, RC3, RJ1, and RG2 were found to produce highest of bacteria cellulose (approximately 9-11 mm) from 1% (v/v) concentration of acetic acid in HS broth. The morphology and nanofibers were investigated by SEM and XRD, the bacterial cellulose nanofibrils size was 4.10, 4.95, 4.38, and 4.91 nm, respectively. However, isolates RJ1, RC3, and RC1 were identified to Komagataeibacter hansenii and then, isolates RG2 was identified to K. maltaceti.
DOI: 10.14455/tsb.res.2019.41
title: Isolation of Fungi from Agarwood Timber and Chemical Constituents in Agarwood Oil

description: Aquilaria crassna (agar wood) is an important economic plant that is traded at the global level due to its high value and demand. The extracted essential oils from agarwood are widely used as traditional medicines, incense in religious ceremonies and perfumes. The chemical constituents in agar wood oil were analyzed by gas chromatography mass spectrometry (GC-MS). In this study, methyl hept-2-ynoate was found to be a major component, coming in at 7. 39 percent. In order to better understand the role of fungi in agarwood formation, fungi in agarwood timber from Chanthaburi Province were determined using a direct DNA sequencing with the specific primer of 18S rRNA-based bioinformatic methods. When comparing DNA sequences in the NCBI database, fungi in agarwood, namely Phoma herbarum showed the highest percentage identity (96.06% ) . The potential of fungi isolated from agarwood timber will be evaluated for inducing agarwood production by the artificial inoculation method.
DOI: 10.14455/tsb.res.2019.42
title: Isolation of potential lignin-degrading bacteria from tropical forest soils in Thailand

description: Lignin is the second most abundant polymer in plant residues and nature in general. However, lignin recalcitrance poses a challenge to biofuel production from plant biomass. To overcome the problem, this research aims to isolate bacteria strains capable of degrading lignin. Potential ligninolytic bacteria strains were isolated from 16 different soil samples based on their ability to grow on minimal media with alkali lignin as sole carbon source at 40?C under microaerobic conditions. Furthermore, growth on model lignin compounds (guaiacol and veratryl alcohol) and dyes (aniline blue, methylene blue, and Congo red) was also used to isolate four potential ligninolytic strains. Results from 16s rDNA sequence analysis showed that the four isolates belonged to genus Burkholderia, Pseudomonas, and Klebsiella. The gene encoding laccase-like multicopper oxidase was only detected in Burkholderia. Thus, the isolates identified in this study are capable of degrading lignin and may possess genes encoding for different ligninolytic enzymes which can be further explored for lignin degradation and optimization of biofuel production from plant biomass.
DOI: 10.14455/tsb.res.2019.43
title: Metformin Suppresses Epithelial Cell Adhesion Through ROS Production Involving Apoptosis in Cervical Cancer Cells

description: Cervical cancer is one of the most prevalent cancer among female after breast cancer and its mortality rate is the highest in women malignant tumor of reproductive system. Epidemiological study indicates that metformin, an anti-diabetes drug, has an anti-proliferation effect with apoptotic-response mitochondrial signaling cascade in various cancers. However, the effect of metformin-mediated apoptosis involves in epithelial cell adhesion is not well characterized. Therefore, the mechanism underlying metformin-induced apoptosis related to epithelial cell adhesion expression in HeLa cells was investigated. The anti-proliferation effect of metformin in the HeLa cells was determined by MTT assay. Cell apoptosis was detected using Flow cytometry assay. The levels of E-cadherin (epithelial cell adhesion marker) expression were performed using Western blot analysis as well as the intracellular ROS levels were measured by DCFH-DA fluorimetric assay. The results revealed that metformin inhibited cell proliferation and induced cell apoptosis. Moreover, metformin suppressed the expression of E-cadherin through the increased ROS generation that was completely rescued by Nacetyl cysteine (NAC), ROS scavenger. Taken together, our results suggested that metformin-mediated apoptosis involved in the elevated ROS suppressing the expression of epithelial cell adhesion.