cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.g3x783
title: Occurrence Download

description: A dataset containing 44 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aquilegia vulgaris",
 "Country is Romania",
 "HasCoordinate is true"
 ]
}

 The dataset includes 44 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045407-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/gvwn3-61717
title: U937 JV9082

description: Legionella pneumophila JV9298 DotF-GFP
description: <strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-4/3dimage_123617/</p>U937_9082_tomo_4_v2_SIRT_1k.rec,  Reconstruction (Pixel Size 1.06 nm), 4.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-4/3dimage_123617/U937_9082_tomo_4_v2_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        U937_9082_tomo_4_v2_bin2.st,  Tilt Series (Pixel Size 0.53 nm), 660.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-4/rawdata/U937_9082_tomo_4_v2_bin2.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8ekf45
title: Occurrence Download

description: A dataset containing 4230 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aquilegia vulgaris",
 "Country is Russian Federation",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4230 records from 31 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045408-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/zkgy3-ybx36
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-05</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-3/rawdata/</p>targ03.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-3/rawdata/targ03.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ03_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-3/3dimage_99235/targ03_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cm394-6sh13
title: Legionella delta DotO

description: Strain from Yale
description: <strong>Tilt Series Date:</strong> 2019-01-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jun Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase, broad vitrobot, R2/2 quantifoil 200 mesh copper grid</p>
<strong>Sample Preparation:</strong> 10 nm gold beads</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-17/rawdata/</p>tilt_78-half1.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-17/rawdata/tilt_78-half1.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_78-half1_full.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-17/3dimage_101013/tilt_78-half1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/km7af-jzv40
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-47/rawdata/</p>caulobacter_na1000_wt_048_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-47/rawdata/caulobacter_na1000_wt_048_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_048_af.mrc.mdoc,  815.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-47/rawdata/caulobacter_na1000_wt_048_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hq847-sa516
title: gx1-2_sq2_cell2

description: GX1-2 - Sq 2 - 1.4s
description: <strong>Tilt Series Date:</strong> 2018-11-01</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter xylinus</p>
<strong>Strain:</strong> JCM 9730</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 102.0 eV/Å²,  defocus: -3.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight culture - centrif 2500rcf, 10min, 30C.Resuspended pellet in 0.5mL at 10:45am</p>
<strong>Sample Preparation:</strong> Freezing at 4:10pm (5:30 incubation).Quantifoil Cu NH2 Finder R2/2 Thick carbon, Glowdischarged 15mA - 1minManual blotting 5s, glare passed at 3s</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-11-01-5/rawdata/</p>gx1-2_sq2_cell2.mrc,  Tilt Series (Pixel Size 0.54 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-11-01-5/rawdata/gx1-2_sq2_cell2.mrc"
        > <i class="download icon"></i> Download </a></p>

        gx1-2_sq2_cell2_full.rec,  Reconstruction (Pixel Size 2.16 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-11-01-5/3dimage_98414/gx1-2_sq2_cell2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4cs1e-snp06
title: JV9082 U937 infection

description: Lp DotF-GFP JV9082 infecting U937
description: <strong>Tilt Series Date:</strong> 2020-10-29</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> JV9082 O.D.=2.5, U937 72h post TPA treatment.</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 10nm gold, 95% humidity, 37 deg C, manual double blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-29-4/rawdata/</p>Lp_5.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-29-4/rawdata/Lp_5.mrc"
        > <i class="download icon"></i> Download </a></p>

        Lp_5_full.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-29-4/3dimage_114946/Lp_5_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8ve5y-82f98
title: Caulobacter delta-CpaF

description: Raw data files of Caulobacter delta-CpaF
description: <strong>Tilt Series Date:</strong> 2019-01-11</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 183.0 eV/Å²,  defocus: -6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> iMOD</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 28 degrees celsius</p>
<strong>Sample Preparation:</strong> plunge-freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-01-11-11/rawdata/</p>targ20.mrc,  Tilt Series (Pixel Size 0.351 nm), 910.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-01-11-11/rawdata/targ20.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ20_full.rec,  Reconstruction (Pixel Size 1.404 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-01-11-11/3dimage_100959/targ20_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5490x-sz572
title: Legionella delta DotO

description: Strain from Yale
description: <strong>Tilt Series Date:</strong> 2019-01-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jun Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase, broad vitrobot, R2/2 quantifoil 200 mesh copper grid</p>
<strong>Sample Preparation:</strong> 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-21/rawdata/</p>tilt_74.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-21/rawdata/tilt_74.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_74_part61_2.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-21/3dimage_101021/tilt_74_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j3m9c-t5c56
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-11-14</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, WT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-22/rawdata/</p>targ22.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-22/rawdata/targ22.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ22_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-22/3dimage_99697/targ22_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2xs5b-4wm80
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-174/rawdata/</p>caulobacter_na1000_wt_176_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-174/rawdata/caulobacter_na1000_wt_176_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_176_af.mrc.mdoc,  800.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-174/rawdata/caulobacter_na1000_wt_176_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g34bm-cex91
title: P. gingivalis wild type

description: Raw data files of P. gingivalis wild type
description: <strong>Tilt Series Date:</strong> 2020-11-06</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown and harvested from plates</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-231/rawdata/</p>PginWT9_230.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-231/rawdata/PginWT9_230.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT9_230_part41_3.rec,  Reconstruction (Pixel Size 1.332 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-231/3dimage_115431/PginWT9_230_part41_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xy7ah-c9y41
title: Shewanella oneidensis dFlgH

description: Raw data files of Shewanella oneidensis dFlgH
description: <strong>Tilt Series Date:</strong> 2018-05-27</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Jeff Granlick (Uni. of Minnesota) supplied the mutant cells.</p>
<strong>Sample Preparation:</strong> Samples were prepared from overnight LB culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-4/rawdata/</p>tilt13.mrc,  Tilt Series (Pixel Size 0.57 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-4/rawdata/tilt13.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt13_full.rec,  Reconstruction (Pixel Size 2.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-4/3dimage_93286/tilt13_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6eh1s-y6d10
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP52s
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3871</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP52 M. smegmatisΔ3871   (ΔEcccA1) sauton media @ 37C</p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-31/rawdata/</p>kp52s0008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
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        kp52s0008_part121_1.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
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