cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7186205
title: Establishment of an environmental microbiological monitoring program in a mice barrier facility

description: Abstract Rodents used in biomedical research are maintained behind barriers to exclude microbial contaminants. Several check points have to be monitored to eliminate the potential of introducing adventitious agents into the facility. Microbiological monitoring of a mouse facility environment enables to evaluate the efficiency of sanitization and cleaning procedures, air quality, and technician good practices. At our SPF mouse facility, we implemented an environmental microbiological monitoring program based in sedimentation and swabbing, inexpensive and easy to use methods. The aim of this work was to evaluate the results and the efficiency of the monitoring program after seven years. The median for bacteria and fungi counts in the SPF sampled areas was ≤2 CFU/2 h for settle plates and &lt;1 CFU per swabbing plate, satisfying the requirements for grade C of the EU-GMP, with some modifications. The environmental monitoring program was useful to detect early warning of problems and enabled us to define a safe range of microbiological counts. In addition, SPF status defined for our mice was maintained throughout this study, confirmed by our HM program. This work could encourage directors and technicians of other mouse facilities in Latin America and rest of the world to implement this kind of program.
DOI: 10.7284/114777
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise TN315 using a Hawaii UHDAS instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186202.v1
title: Delayed healthcare and secondary infections following freshwater stingray injuries: risk factors for a poorly understood health issue in the Amazon

description: Abstract INTRODUCTION This study aimed to describe the profile of freshwater stingray injuries in the State of Amazonas, Brazilian Amazon, and to identify the associated risk factors for secondary infections. METHODS This cross-sectional study used surveillance data from 2007 to 2014 to identify factors associated with secondary infections from stingray injuries. RESULTS A total of 476 freshwater stingray injuries were recorded, with an incidence rate of 1.7 cases/100,000 person/year. The majority of injuries were reported from rural areas (73.8%) and 26.1% were related to work activities. A total of 74.5% of patients received medical assistance within the first 3 hours of injury. Secondary infections and necrosis were observed in 8.9% and 3.8%, respectively. Work-related injuries [odds ratio (OR) 4.1, confidence interval (CI); 1.87-9.13] and &gt;24 hours from a sting until receiving medical care (OR; 15.5, CI; 6.77-35.40) were independently associated with the risk of secondary bacterial infection. CONCLUSIONS: In this study, work-related injuries and &gt;24 hours from being stung until receiving medical care were independently and significantly associated with the risk of secondary infection. The frequency of infection following sting injuries was 9%. The major factor associated with the risk of secondary bacterial infection was a time period of &gt;24 hours from being stung until receiving medical care.
DOI: 10.6084/m9.figshare.7186205.v1
title: Establishment of an environmental microbiological monitoring program in a mice barrier facility

description: Abstract Rodents used in biomedical research are maintained behind barriers to exclude microbial contaminants. Several check points have to be monitored to eliminate the potential of introducing adventitious agents into the facility. Microbiological monitoring of a mouse facility environment enables to evaluate the efficiency of sanitization and cleaning procedures, air quality, and technician good practices. At our SPF mouse facility, we implemented an environmental microbiological monitoring program based in sedimentation and swabbing, inexpensive and easy to use methods. The aim of this work was to evaluate the results and the efficiency of the monitoring program after seven years. The median for bacteria and fungi counts in the SPF sampled areas was ≤2 CFU/2 h for settle plates and &lt;1 CFU per swabbing plate, satisfying the requirements for grade C of the EU-GMP, with some modifications. The environmental monitoring program was useful to detect early warning of problems and enabled us to define a safe range of microbiological counts. In addition, SPF status defined for our mice was maintained throughout this study, confirmed by our HM program. This work could encourage directors and technicians of other mouse facilities in Latin America and rest of the world to implement this kind of program.
DOI: 10.6084/m9.figshare.7186199
title: Review of peripheral blood smear slides: assessment of the compliance with criteria used by analysts in a laboratory of a public hospital in Bahia, Brazil

description: ABSTRACT Introduction: Recommendations in the literature and publications of scientific societies have proposed classification systems and levels to report blood cell changes in the complete blood count (CBC). Aiming to standardize the results released in the clinical laboratory, the standardization in hematoscopy is an important action within the quality assurance, contributing for diagnosis and appropriate follow-up of patients. Objective: To verify the adequacy of the classification criteria of the blood smear changes adopted by the laboratory professionals at the General Hospital of Vitória da Conquista, Bahia, Brazil. Material and method: In the 12-week period, 159 slides of CBC were selected from 53 patients from the four intensive care units (ICUs) at the hospital, three slides per patient. The readings of the slides were performed by the analysts on duty and by two other quality analysts-control who used the criteria standardized by Palmer (2015), all analyses were blind and independent. The results were compared using the Cohen's kappa agreement tests between the analysts-control and between the quality control analysts and the analysts on duty. Results: For the analyzed parameters, there was moderate to excellent agreement among the quality analysts-control, and when they were compared to the analysts on duty, there was a slight to substantial agreement. Discussion: The lowest agreement occurred in the expression of poikilocytosis and lymphocytic atypia, which are not detected by the hemocytometer, and the analyst's technical capacity is required. Conclusion: The study evidenced the need of standardization of the criteria for hematoscopy used by analysts to obtain reproducible results that reflect quality and reliability by the prescribing physician. Therefore, it is necessary to invest in training the professionals involved in revision of blood smear slides.
DOI: 10.7284/121060
title: Anemometer data collected during research cruise TN315 using a RM Young 06206 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186196
title: Metal Organic Frameworks for Selective Degradation of Amoxicillin in Biomedical Wastes

description: The accumulation of antibiotics in wastewater has led to the development and spreading of antibiotic resistance in the environment. Amoxicillin (Amox), a beta-lactamic antibiotic, is one of the most frequently consumed antibiotics in the world. We have applied two metal-organic frameworks (MOFs) containing zinc(II) as platforms to degrade Amox. We have predicted the adsorption of this antibiotic via molecular docking calculations which have been further corroborated by means of Fourier transform infrared and UV-Vis spectroscopies, thermogravimetric analysis, X-ray diffraction and scanning microscopy measurements. We have subsequently performed mass spectrometry analysis of Amox@zeolitic imidazolate framework-8 (ZIF-8) and Amox@Zn(1,4-benzenedicarboxylate) (ZnBDC) to demonstrate the degradation of Amox upon contact with the Zn-containing frameworks. We propose a possible pathway for the degradation of Amox involving the cleavage of the four-membered β-lactam ring. These Zn-containing frameworks provide a biocompatible platform for the degradation in solution of Amox, which should also be suitable to degrade other β-lactam antibiotics.
DOI: 10.6084/m9.figshare.7186196.v1
title: Metal Organic Frameworks for Selective Degradation of Amoxicillin in Biomedical Wastes

description: The accumulation of antibiotics in wastewater has led to the development and spreading of antibiotic resistance in the environment. Amoxicillin (Amox), a beta-lactamic antibiotic, is one of the most frequently consumed antibiotics in the world. We have applied two metal-organic frameworks (MOFs) containing zinc(II) as platforms to degrade Amox. We have predicted the adsorption of this antibiotic via molecular docking calculations which have been further corroborated by means of Fourier transform infrared and UV-Vis spectroscopies, thermogravimetric analysis, X-ray diffraction and scanning microscopy measurements. We have subsequently performed mass spectrometry analysis of Amox@zeolitic imidazolate framework-8 (ZIF-8) and Amox@Zn(1,4-benzenedicarboxylate) (ZnBDC) to demonstrate the degradation of Amox upon contact with the Zn-containing frameworks. We propose a possible pathway for the degradation of Amox involving the cleavage of the four-membered β-lactam ring. These Zn-containing frameworks provide a biocompatible platform for the degradation in solution of Amox, which should also be suitable to degrade other β-lactam antibiotics.
DOI: 10.6084/m9.figshare.7186199.v1
title: Review of peripheral blood smear slides: assessment of the compliance with criteria used by analysts in a laboratory of a public hospital in Bahia, Brazil

description: ABSTRACT Introduction: Recommendations in the literature and publications of scientific societies have proposed classification systems and levels to report blood cell changes in the complete blood count (CBC). Aiming to standardize the results released in the clinical laboratory, the standardization in hematoscopy is an important action within the quality assurance, contributing for diagnosis and appropriate follow-up of patients. Objective: To verify the adequacy of the classification criteria of the blood smear changes adopted by the laboratory professionals at the General Hospital of Vitória da Conquista, Bahia, Brazil. Material and method: In the 12-week period, 159 slides of CBC were selected from 53 patients from the four intensive care units (ICUs) at the hospital, three slides per patient. The readings of the slides were performed by the analysts on duty and by two other quality analysts-control who used the criteria standardized by Palmer (2015), all analyses were blind and independent. The results were compared using the Cohen's kappa agreement tests between the analysts-control and between the quality control analysts and the analysts on duty. Results: For the analyzed parameters, there was moderate to excellent agreement among the quality analysts-control, and when they were compared to the analysts on duty, there was a slight to substantial agreement. Discussion: The lowest agreement occurred in the expression of poikilocytosis and lymphocytic atypia, which are not detected by the hemocytometer, and the analyst's technical capacity is required. Conclusion: The study evidenced the need of standardization of the criteria for hematoscopy used by analysts to obtain reproducible results that reflect quality and reliability by the prescribing physician. Therefore, it is necessary to invest in training the professionals involved in revision of blood smear slides.
DOI: 10.6084/m9.figshare.7186193
title: Creative activities, smart specialization, and opportunities for small urban areas: the Estarreja’s carnival case

description: Abstract In the context of consolidation of a society of knowledge, learning, and creativity, urban territories have had to readjust their strategies around creative activities, constituting new logic of territorial development and public policy. Building on the contextualization of smart specialization within the framework of European public policies and the importance of creative activities and resources, this study intends to identify new opportunities for small urban areas in order to redefine their local development strategies. The study aims to promote a debate on the valorization of cultural and creative dynamics that promote integrated and transversal development of the different actors and their territory. The municipality of Estarreja, Portugal, was used as a reference in the context of a small urban space with a past trajectory of industrial specialization, but with some current creative dynamics. In fact, this study intends to discuss the feasibility of applying this strategic matrix within the scope of current public development policies.
DOI: 10.7284/114783
title: CTD (Conductivity, Temperature, Depth) data collected during research cruise TN315 using a Sea-Bird SBE-911+ instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186193.v1
title: Creative activities, smart specialization, and opportunities for small urban areas: the Estarreja’s carnival case

description: Abstract In the context of consolidation of a society of knowledge, learning, and creativity, urban territories have had to readjust their strategies around creative activities, constituting new logic of territorial development and public policy. Building on the contextualization of smart specialization within the framework of European public policies and the importance of creative activities and resources, this study intends to identify new opportunities for small urban areas in order to redefine their local development strategies. The study aims to promote a debate on the valorization of cultural and creative dynamics that promote integrated and transversal development of the different actors and their territory. The municipality of Estarreja, Portugal, was used as a reference in the context of a small urban space with a past trajectory of industrial specialization, but with some current creative dynamics. In fact, this study intends to discuss the feasibility of applying this strategic matrix within the scope of current public development policies.
DOI: 10.6084/m9.figshare.7186187
title: Alkaline phosphatase: reference interval transference from CALIPER to a pediatric Brazilian population

description: ABSTRACT Introduction: Interpreting laboratory tests requires reference intervals (RI) that may vary between different populations. For the diagnosis of hypophosphatasia (HPP), lower limits of alkaline phosphatase (ALP) levels must be determined. Objective: To transfer the RI findings for ALP obtained by the Canadian Laboratory Initiative in Pediatric Reference Intervals (CALIPER) in children and adolescents, adjusted for the Brazilian population. Methods: The ALP measures from 1690 subjects (aging from 1-18 years) were analyzed. The CALIPER subgroups and the Clinical and Laboratory Standards Institute (CLSI) guideline were used for validation. Inclusion criteria were patients with normal range of hepatic and renal function, bone metabolism, and blood counts. Exclusion criteria were hospitalization, low weight, and use of drugs that could interfere in the ALP measurement and patients in with more than three orders for ALP measuring test. The RI obtained were considered valid if more than 90% of patients were whitin of the CALIPER RI. Results: The ALP RI results (IU/l) obtained were: 149-301 for both sexes aged 1-9 years; 127-326 for both sexes aged 10-12 years; 62-212 for girls and 129-437 for boys aged 13-14 years; 52-120 for girls and 78-268 for boys aged 15-16 years; 45-97 for girls and 40-129 for boys aged 17-18 years. In 92.4% of the patients, the results were comparable with those of the CALIPER study. Conclusion: The results demonstrated that the ALP RI for Brazilian children and adolescents are comparable to the CALIPER study in 92.4% of the patients and can be used for this population.
DOI: 10.7284/114779
title: Expendable Probe data collected during research cruise TN315 using a Sippican MK21 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186181
title: Preventable deaths in children under five in the Macro Norte region of the state of Minas Gerais, Brazil

description: Abstract This is a descriptive epidemiological study, with objective of evaluating preventable deaths of children under the age of 5 years which occurred in 2013 in northern macro-region of Minas Gerais. There were 3,641 preventable deaths in Minas Gerais in year studied, 392 of them located in the northern macro-region. The study found a prevalence of preventable deaths in male children of brown colour. Deaths according to age group are also noteworthy.
DOI: 10.6084/m9.figshare.7186187.v1
title: Alkaline phosphatase: reference interval transference from CALIPER to a pediatric Brazilian population

description: ABSTRACT Introduction: Interpreting laboratory tests requires reference intervals (RI) that may vary between different populations. For the diagnosis of hypophosphatasia (HPP), lower limits of alkaline phosphatase (ALP) levels must be determined. Objective: To transfer the RI findings for ALP obtained by the Canadian Laboratory Initiative in Pediatric Reference Intervals (CALIPER) in children and adolescents, adjusted for the Brazilian population. Methods: The ALP measures from 1690 subjects (aging from 1-18 years) were analyzed. The CALIPER subgroups and the Clinical and Laboratory Standards Institute (CLSI) guideline were used for validation. Inclusion criteria were patients with normal range of hepatic and renal function, bone metabolism, and blood counts. Exclusion criteria were hospitalization, low weight, and use of drugs that could interfere in the ALP measurement and patients in with more than three orders for ALP measuring test. The RI obtained were considered valid if more than 90% of patients were whitin of the CALIPER RI. Results: The ALP RI results (IU/l) obtained were: 149-301 for both sexes aged 1-9 years; 127-326 for both sexes aged 10-12 years; 62-212 for girls and 129-437 for boys aged 13-14 years; 52-120 for girls and 78-268 for boys aged 15-16 years; 45-97 for girls and 40-129 for boys aged 17-18 years. In 92.4% of the patients, the results were comparable with those of the CALIPER study. Conclusion: The results demonstrated that the ALP RI for Brazilian children and adolescents are comparable to the CALIPER study in 92.4% of the patients and can be used for this population.
DOI: 10.6084/m9.figshare.7186181.v1
title: Preventable deaths in children under five in the Macro Norte region of the state of Minas Gerais, Brazil

description: Abstract This is a descriptive epidemiological study, with objective of evaluating preventable deaths of children under the age of 5 years which occurred in 2013 in northern macro-region of Minas Gerais. There were 3,641 preventable deaths in Minas Gerais in year studied, 392 of them located in the northern macro-region. The study found a prevalence of preventable deaths in male children of brown colour. Deaths according to age group are also noteworthy.
DOI: 10.7284/114784
title: GNSS (Global Navigation Satellite System) data collected during research cruise TN315 using a C&C C-Nav 3050 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186178
title: Gender and public policy: an overview of academic research in Brazil (1983- 2015)

description: Abstract Public policy for gender equality arose in Brazil in the 1980s. It has gained relevance since 2003, due to the institutionalization of the Secretariat of Policies for Women. However, it does not mean that there is a consolidated research agenda on gender and public policy themes in the country. This article aims to investigate how those themes have been incorporated, in an articulated manner, by academic research in Brazil. To do this, 349 articles available on the SCIELO and SPELL platforms between 1983 and 2015 were analyzed. From this analysis, a heterogeneous set of studies was identified, classified according to a typology built from the standards of incorporation of gender and public policy issues. Each type analysis took also into consideration: distribution of authorship; knowledge areas and the journals where the articles were published; themes and the population segments studied; and methodological approaches applied. It is argued that there is not a constituted research field on that subject, but rather points of convergence between the gender studies and the public policy field. This study aims to contribute to the development of a research agenda which articulates those two fields, and to assist other research based on different periods and data sources, through the methodology developed.
DOI: 10.6084/m9.figshare.7186178.v1
title: Gender and public policy: an overview of academic research in Brazil (1983- 2015)

description: Abstract Public policy for gender equality arose in Brazil in the 1980s. It has gained relevance since 2003, due to the institutionalization of the Secretariat of Policies for Women. However, it does not mean that there is a consolidated research agenda on gender and public policy themes in the country. This article aims to investigate how those themes have been incorporated, in an articulated manner, by academic research in Brazil. To do this, 349 articles available on the SCIELO and SPELL platforms between 1983 and 2015 were analyzed. From this analysis, a heterogeneous set of studies was identified, classified according to a typology built from the standards of incorporation of gender and public policy issues. Each type analysis took also into consideration: distribution of authorship; knowledge areas and the journals where the articles were published; themes and the population segments studied; and methodological approaches applied. It is argued that there is not a constituted research field on that subject, but rather points of convergence between the gender studies and the public policy field. This study aims to contribute to the development of a research agenda which articulates those two fields, and to assist other research based on different periods and data sources, through the methodology developed.