cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11839455
title: Influence of test tube sealing on the morpho-anatomy and ultrastructure of leaves of Aechmea bromeliifolia (Bromeliaceae) grown in vitro

description: Abstract In vitro grown plants may have abnormal structural and physiological features. However, the type of the sealing material used in tissue culture may minimize such abnormalities. This study evaluates the influence of the type of sealing of test tubes on the anatomical and ultrastructural features of leaves of Aechemea bromeliifolia (Rudge) Baker (Bromeliaceae), an ornamental bromeliad native to Brazil, grown in vitro. Three types of sealing were used: rigid polypropylene cap (PC), polyvinyl chloride film (PVC), and PC coupled with a microporous membrane (PM). Seedlings germinated in a greenhouse were also studied for comparison. Plants grown in test tubes sealed with PM were more similar to those from the greenhouse, as far as the pattern of stomatal opening, the presence of starch grains, and the organization of the internal membrane system of the chloroplasts is concerned. Plants cultivated in test tubes sealed with PC had higher stomatal density and the chloroplasts had large areas without thylakoids in the stroma. Plants grown in test tubes sealed with PVC had few or no starch grains. These results suggest that microporous membrane used coupled with PC sealing provided natural ventilation, thus contributing to a better plant development.
DOI: 10.6084/m9.figshare.11839455.v1
title: Influence of test tube sealing on the morpho-anatomy and ultrastructure of leaves of Aechmea bromeliifolia (Bromeliaceae) grown in vitro

description: Abstract In vitro grown plants may have abnormal structural and physiological features. However, the type of the sealing material used in tissue culture may minimize such abnormalities. This study evaluates the influence of the type of sealing of test tubes on the anatomical and ultrastructural features of leaves of Aechemea bromeliifolia (Rudge) Baker (Bromeliaceae), an ornamental bromeliad native to Brazil, grown in vitro. Three types of sealing were used: rigid polypropylene cap (PC), polyvinyl chloride film (PVC), and PC coupled with a microporous membrane (PM). Seedlings germinated in a greenhouse were also studied for comparison. Plants grown in test tubes sealed with PM were more similar to those from the greenhouse, as far as the pattern of stomatal opening, the presence of starch grains, and the organization of the internal membrane system of the chloroplasts is concerned. Plants cultivated in test tubes sealed with PC had higher stomatal density and the chloroplasts had large areas without thylakoids in the stroma. Plants grown in test tubes sealed with PVC had few or no starch grains. These results suggest that microporous membrane used coupled with PC sealing provided natural ventilation, thus contributing to a better plant development.
DOI: 10.6084/m9.figshare.11839476
title: The genus Senna (Leguminosae-Caesalpinioideae) in the municipality of Caetité, Bahia, Brazil

description: Abstract A taxonomic study of the genus Senna in the municipality of Caetité, Bahia is presented as a part of the Project Flora of Caetité. This municipality presents a diverse landscape in terms vegetational aspects, with areas of Caatinga, Rupestrian field, Cerrado, and Gallery vegetation, influenced by soil types, and the elevation and inclination of the land. Specimens were collected were collected in the period from February 2015 to June 2016 during random walks through the area. In addition to the plants collected in the field, specimens from the HUEFS and HUNEB/Collection Caetité herbaria were also analyzed. A total of 14 taxa (13 species) were recognized, of which Senna cana var. hypoleuca, S. occidentalis, S. rizzinii, S. spectabilis var. excelsa, and S. uniflora are new records in the study area. Among the taxa found, three are endemic to the Brazilian northeast: S. acuruensis, S. aversiflora, and S. rizzinii. This work comprises identification key, descriptions, illustrations, taxonomic comments, geographical distribution and phenology of all taxa.
DOI: 10.6084/m9.figshare.11839476.v1
title: The genus Senna (Leguminosae-Caesalpinioideae) in the municipality of Caetité, Bahia, Brazil

description: Abstract A taxonomic study of the genus Senna in the municipality of Caetité, Bahia is presented as a part of the Project Flora of Caetité. This municipality presents a diverse landscape in terms vegetational aspects, with areas of Caatinga, Rupestrian field, Cerrado, and Gallery vegetation, influenced by soil types, and the elevation and inclination of the land. Specimens were collected were collected in the period from February 2015 to June 2016 during random walks through the area. In addition to the plants collected in the field, specimens from the HUEFS and HUNEB/Collection Caetité herbaria were also analyzed. A total of 14 taxa (13 species) were recognized, of which Senna cana var. hypoleuca, S. occidentalis, S. rizzinii, S. spectabilis var. excelsa, and S. uniflora are new records in the study area. Among the taxa found, three are endemic to the Brazilian northeast: S. acuruensis, S. aversiflora, and S. rizzinii. This work comprises identification key, descriptions, illustrations, taxonomic comments, geographical distribution and phenology of all taxa.
DOI: 10.6084/m9.figshare.11839902.v1
title: Bambusoideae (Poaceae) in the Serra do Brigadeiro State Park, Minas Gerais, Brazil

description: Abstract This study consists of a floristic survey of the Bambusoideae (Poaceae) species that occur in the Serra do Brigadeiro State Park (PESB). The study area is inserted in the Atlantic Forest, considered as one of the centers of bamboo endemism. We carried out expeditions from August 2013 to July 2015 for the collection of botanical material and observation of the species in their natural environment. In addition, the collections of the herbaria BHCB, CESJ, HUEFS, ISC, K, MG, RB, SPF, VIC, and VIES were analyzed. Six genera and 27 species were recorded in the PESB. The most diverse genus is Chusquea with 16 species, followed by Merostachys and Aulonemia with four species each, and Colanthelia, Guadua, and Parodiolyra with one species each. Identification keys for genera and species, descriptions, and morphological comments are presented. PESB has the highest bamboo species richness recorded for the conservation units of Minas Gerais.
DOI: 10.6084/m9.figshare.11839902
title: Bambusoideae (Poaceae) in the Serra do Brigadeiro State Park, Minas Gerais, Brazil

description: Abstract This study consists of a floristic survey of the Bambusoideae (Poaceae) species that occur in the Serra do Brigadeiro State Park (PESB). The study area is inserted in the Atlantic Forest, considered as one of the centers of bamboo endemism. We carried out expeditions from August 2013 to July 2015 for the collection of botanical material and observation of the species in their natural environment. In addition, the collections of the herbaria BHCB, CESJ, HUEFS, ISC, K, MG, RB, SPF, VIC, and VIES were analyzed. Six genera and 27 species were recorded in the PESB. The most diverse genus is Chusquea with 16 species, followed by Merostachys and Aulonemia with four species each, and Colanthelia, Guadua, and Parodiolyra with one species each. Identification keys for genera and species, descriptions, and morphological comments are presented. PESB has the highest bamboo species richness recorded for the conservation units of Minas Gerais.
DOI: 10.6084/m9.figshare.11839896
title: Tree species richness and composition in a fragmented landscape of the Brazilian lowland Atlantic Forest

description: Abstract Tropical forests are one of the most important ecosystems and loss of biological diversity in these is a major concern. We studied seven forest remnants on the coast of Rio de Janeiro, southeastern Brazil, three medium-sized (24-63 ha) and four small (5-11 ha), to assess the influence of size, degree of disturbance and isolation on composition and diversity of the tree layer. A total of 443 species in 60 families and 185 genera were recorded, with Myrtaceae, Lauraceae and Fabaceae being the most species-rich families. The results showed areas of high α-diversity (α= 34.86-75.69) and a slightly greater decline of similarity with distance between the study areas. Remnant size not explained the variation in the species composition and we found correlation between the level of disturbance and the floristic composition. The remnants had low floristic similarity, strongly influenced by the degree of disturbance. Results suggest that disturbance influences composition and diversity and confirm the importance of including both medium-sized and small remnants in the conservation of tropical forest diversity.
DOI: 10.6084/m9.figshare.11839896.v1
title: Tree species richness and composition in a fragmented landscape of the Brazilian lowland Atlantic Forest

description: Abstract Tropical forests are one of the most important ecosystems and loss of biological diversity in these is a major concern. We studied seven forest remnants on the coast of Rio de Janeiro, southeastern Brazil, three medium-sized (24-63 ha) and four small (5-11 ha), to assess the influence of size, degree of disturbance and isolation on composition and diversity of the tree layer. A total of 443 species in 60 families and 185 genera were recorded, with Myrtaceae, Lauraceae and Fabaceae being the most species-rich families. The results showed areas of high α-diversity (α= 34.86-75.69) and a slightly greater decline of similarity with distance between the study areas. Remnant size not explained the variation in the species composition and we found correlation between the level of disturbance and the floristic composition. The remnants had low floristic similarity, strongly influenced by the degree of disturbance. Results suggest that disturbance influences composition and diversity and confirm the importance of including both medium-sized and small remnants in the conservation of tropical forest diversity.
DOI: 10.6084/m9.figshare.11839893.v1
title: Myrtaceae from the Atlantic forest subtropical highlands of São Joaquim National Park (Santa Catarina, Brazil)

description: Abstract Studies that address the floristic composition of the Atlantic forest show that Myrtaceae is one of the richest families in number of tree species. In Southern Brazil, Myrtaceae is present mainly in forest formations, such as broadleaf rain forests and broadleaf cloud forests, mixed needle and broadleaf forests (Araucaria forests), and seasonal semi-deciduous forests. The objective of this study was to survey the Myrtaceae of the upper highlands of São Joaquim National Park, to contribute with the knowledge of its flora. Eight genera and 16 species of Myrtaceae were found: Acca sellowiana, Blepharocalyx salicifolius, Eugenia handroi, Myrceugenia (8 spp.): M. acutata, M. alpigena, M. euosma, M. miersiana, M. myrcioides, M. oxysepala, M. pilotantha, and M. regnelliana, Myrcia (2 spp.): M. aethusa and M. hartwegiana, Myrrhinium atropurpureum, Psidium salutare, and Siphoneugena reitzii. Descriptions, illustrations, an identification key and commentaries are presented. Other studies about Myrtaceae in highland areas of Southern Brazil present Myrceugenia as the richest genus in species, while other mentioned genera were not registered in SJNP upper highlands. The family’s richness patterns and diversity in a local and regional scale should be better investigated in the Atlantic Forest.
DOI: 10.6084/m9.figshare.11839893
title: Myrtaceae from the Atlantic forest subtropical highlands of São Joaquim National Park (Santa Catarina, Brazil)

description: Abstract Studies that address the floristic composition of the Atlantic forest show that Myrtaceae is one of the richest families in number of tree species. In Southern Brazil, Myrtaceae is present mainly in forest formations, such as broadleaf rain forests and broadleaf cloud forests, mixed needle and broadleaf forests (Araucaria forests), and seasonal semi-deciduous forests. The objective of this study was to survey the Myrtaceae of the upper highlands of São Joaquim National Park, to contribute with the knowledge of its flora. Eight genera and 16 species of Myrtaceae were found: Acca sellowiana, Blepharocalyx salicifolius, Eugenia handroi, Myrceugenia (8 spp.): M. acutata, M. alpigena, M. euosma, M. miersiana, M. myrcioides, M. oxysepala, M. pilotantha, and M. regnelliana, Myrcia (2 spp.): M. aethusa and M. hartwegiana, Myrrhinium atropurpureum, Psidium salutare, and Siphoneugena reitzii. Descriptions, illustrations, an identification key and commentaries are presented. Other studies about Myrtaceae in highland areas of Southern Brazil present Myrceugenia as the richest genus in species, while other mentioned genera were not registered in SJNP upper highlands. The family’s richness patterns and diversity in a local and regional scale should be better investigated in the Atlantic Forest.
DOI: 10.6084/m9.figshare.12171669.v1
title: Peperomia (Piperaceae) in the Parque Estadual de Vila Velha, Paraná.

description: Abstract Peperomia is a pantropical genus and is represented in Brazil by 171 species that occur in the phytogeographical domains of the Amazon, Caatinga, Cerrado and Atlantic Forest. A taxonomic study for the genus in the Parque Estadual de Vila Velha was carried out through field trips, visits to herbaria and specialized literature. A total of 16 species were confirmed in the study area. The species Peperomia crinicaulis, P. hilariana, P. subrubrispica and P. velloziana are endemic to Brazil. Six species presented are new occurrences for this Conservation Unit. This study presents key identification, descriptions, comments and illustrations of the species of Peperomia occurring in the study area.
DOI: 10.6084/m9.figshare.12171669
title: Peperomia (Piperaceae) in the Parque Estadual de Vila Velha, Paraná.

description: Abstract Peperomia is a pantropical genus and is represented in Brazil by 171 species that occur in the phytogeographical domains of the Amazon, Caatinga, Cerrado and Atlantic Forest. A taxonomic study for the genus in the Parque Estadual de Vila Velha was carried out through field trips, visits to herbaria and specialized literature. A total of 16 species were confirmed in the study area. The species Peperomia crinicaulis, P. hilariana, P. subrubrispica and P. velloziana are endemic to Brazil. Six species presented are new occurrences for this Conservation Unit. This study presents key identification, descriptions, comments and illustrations of the species of Peperomia occurring in the study area.
DOI: 10.6084/m9.figshare.12171654
title: Dunes in the North coast of Sergipe, Brazil: plant species and their ecological traits

description: Abstract Restinga vegetation, which is a vegetation type of coastal zones, are present in two geomorphological features in the North coast of Sergipe state - Brazil: litoraneous dunes (LD) and continental dunes (CD). The objective of the present paper is to record the species composition, Raunkiaer’s life forms, dispersal syndromes and soil fertility differences between both habitats. Furthermore, we compared the life forms spectra of the studied features to the spectra of the main Brazilian phytogeographic domains. We conducted a floristic survey from November 2013 to May 2014 in different Restinga areas in two dune habitats in coastal Sergipe state, Brazil, and compared differences in the two localities. Although the two areas were geographically close, there was low floristic similarity between the two habitats; CD showed higher richness than LD. The spectrum of life forms was more similar to that of Caatinga, probably due to environmental similarities. Life forms and dispersal syndromes proportions significantly differed between features, as well as the pH, organic matter, phosphorus, sodium and base saturation index. Zoochory prevailed in both features, whereas phanerophytes prevailed in CD and chamaephytes and phanerophytes in LD. Despite the influence of historical processes, micro-environmental differences between features, which were amplified by their different distances from the sea, were assumingly responsible for the recorded differences.
DOI: 10.6084/m9.figshare.12171654.v1
title: Dunes in the North coast of Sergipe, Brazil: plant species and their ecological traits

description: Abstract Restinga vegetation, which is a vegetation type of coastal zones, are present in two geomorphological features in the North coast of Sergipe state - Brazil: litoraneous dunes (LD) and continental dunes (CD). The objective of the present paper is to record the species composition, Raunkiaer’s life forms, dispersal syndromes and soil fertility differences between both habitats. Furthermore, we compared the life forms spectra of the studied features to the spectra of the main Brazilian phytogeographic domains. We conducted a floristic survey from November 2013 to May 2014 in different Restinga areas in two dune habitats in coastal Sergipe state, Brazil, and compared differences in the two localities. Although the two areas were geographically close, there was low floristic similarity between the two habitats; CD showed higher richness than LD. The spectrum of life forms was more similar to that of Caatinga, probably due to environmental similarities. Life forms and dispersal syndromes proportions significantly differed between features, as well as the pH, organic matter, phosphorus, sodium and base saturation index. Zoochory prevailed in both features, whereas phanerophytes prevailed in CD and chamaephytes and phanerophytes in LD. Despite the influence of historical processes, micro-environmental differences between features, which were amplified by their different distances from the sea, were assumingly responsible for the recorded differences.
DOI: 10.6084/m9.figshare.12171540
title: Morphoanatomy of vegetative organs of two species of Cattleya (Orchidaceae) native to Brazil

description: Abstract Orchidaceae is the largest family of vascular plants and is a group under intense threat due to anthropic activities. Cattleya caulescens and C. endsfeldzii are endemic rupiculous species of Minas Gerais state, Brazil, and listed on Red Lists of endangered species at state and national levels. We conducted morphoanatomical studies with the vegetative organs of the two species, with the objective of increasing knowledge of these taxa, considering the potential that these studies represent in the management of species. Cattleya caulescens and C. endsfeldzii show strong morphoanatomical similarities, which corroborates with previous studies that include both species in the same section of the genus. Many anatomical features described here (presence of single-layered epidermis, stomata only on the abaxial side of the leaf, large primary pit fields in the chlorophyll parenchyma and root anatomy) are common to several other species of Orchidaceae. The xeromorphic features observed in C. caulescens and C. endsfeldzii are related to edaphoclimatic conditions, since they grow on rocky substrate, in constant exposure to solar light and severe water restriction during most of the year. This work contributes to increase the knowledge about Cattleya genus and to support in the taxonomic delimitation of these endangered species occurring in areas of intense antropic activity.
DOI: 10.6084/m9.figshare.12171540.v1
title: Morphoanatomy of vegetative organs of two species of Cattleya (Orchidaceae) native to Brazil

description: Abstract Orchidaceae is the largest family of vascular plants and is a group under intense threat due to anthropic activities. Cattleya caulescens and C. endsfeldzii are endemic rupiculous species of Minas Gerais state, Brazil, and listed on Red Lists of endangered species at state and national levels. We conducted morphoanatomical studies with the vegetative organs of the two species, with the objective of increasing knowledge of these taxa, considering the potential that these studies represent in the management of species. Cattleya caulescens and C. endsfeldzii show strong morphoanatomical similarities, which corroborates with previous studies that include both species in the same section of the genus. Many anatomical features described here (presence of single-layered epidermis, stomata only on the abaxial side of the leaf, large primary pit fields in the chlorophyll parenchyma and root anatomy) are common to several other species of Orchidaceae. The xeromorphic features observed in C. caulescens and C. endsfeldzii are related to edaphoclimatic conditions, since they grow on rocky substrate, in constant exposure to solar light and severe water restriction during most of the year. This work contributes to increase the knowledge about Cattleya genus and to support in the taxonomic delimitation of these endangered species occurring in areas of intense antropic activity.
DOI: 10.6084/m9.figshare.12171567.v1
title: An inexpensive and environmentally friendly staining method for semi-permanent slides from plant material probed using anatomical and computational chemistry analyses

description: Abstract One of the main methods for plant anatomy study is the analysis of thin, transparent, and stained tissue sections. Synthetic dyes traditionally used in anatomical studies might be expensive and produced by specific companies. In contrast, the use of alternative industrial dyes can both represent an inexpensive substitute as well as an environmentally friendly option for conducting plant anatomy studies. In this study, a set of 22 textile dyes was evaluated. Transversal-, longitudinal, and paradermal sections of plant organs obtained using the freehand cutting technique were stained using hydroalcoholic solution (0 to 100%) of textile dyes purchased from a local market. Dyes mixed with 50% hydroalcoholic solution showed higher efficiency in tissue contrast, allowing greater solubility of dye powder and better solution interaction with the plant tissues. Most of the tested dyes showed satisfactory staining results. Cell wall, especially lignified one, showed higher staining efficiency. Computational docking analysis and molecular models of cellulose and lignin showed the probable association mechanisms and dye selectivity to cell wall constituents. Our findings suggest that the developed method can be useful in mixed practical classes of plant anatomy, chemistry, and/or biochemistry, both at high school as well as undergraduate levels.
DOI: 10.6084/m9.figshare.12171567
title: An inexpensive and environmentally friendly staining method for semi-permanent slides from plant material probed using anatomical and computational chemistry analyses

description: Abstract One of the main methods for plant anatomy study is the analysis of thin, transparent, and stained tissue sections. Synthetic dyes traditionally used in anatomical studies might be expensive and produced by specific companies. In contrast, the use of alternative industrial dyes can both represent an inexpensive substitute as well as an environmentally friendly option for conducting plant anatomy studies. In this study, a set of 22 textile dyes was evaluated. Transversal-, longitudinal, and paradermal sections of plant organs obtained using the freehand cutting technique were stained using hydroalcoholic solution (0 to 100%) of textile dyes purchased from a local market. Dyes mixed with 50% hydroalcoholic solution showed higher efficiency in tissue contrast, allowing greater solubility of dye powder and better solution interaction with the plant tissues. Most of the tested dyes showed satisfactory staining results. Cell wall, especially lignified one, showed higher staining efficiency. Computational docking analysis and molecular models of cellulose and lignin showed the probable association mechanisms and dye selectivity to cell wall constituents. Our findings suggest that the developed method can be useful in mixed practical classes of plant anatomy, chemistry, and/or biochemistry, both at high school as well as undergraduate levels.