cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.6223066
title: Raw Data for the article: A retrospective molecular epidemiological scenario of carbapenemase-producing Klebsiella pneumoniae clinical isolates in a Sicilian transplantation hospital shows a swift polyclonal divergence among sequence types, resistome and virulome

description: In this work, we assessed and characterized the epidemiological scenario of carbapenem-resistant Klebsiella pneumoniae strains (CR-Kp) at IRCCS-ISMETT, a transplantation hospital in Palermo, Italy, from 2008 to 2017. A total of 288 K. pneumoniae clinical isolates were selected based on their resistance to carbapenems. Molecular characterization was also done in terms of the presence of virulence and resistance genes. All patients were inpatients from our facility and clinical isolates were collected from several sources, either from infection or colonization cases. We observed that, in agreement with the Italian epidemiological scenario, initially only ST258 and ST512 clade II (but not from clade I) were identified from 2008 to 2011. From 2012 onwards, other STs have been observed, including the clinically relevant ST101 and ST307, but also others not previously observed in other Italian health settings, such as ST220 and ST753. The presence of genes involved in resistance and virulence was confirmed, and a heterogeneous genetic resistance profile throughout the years was observed. Our work highlights that resistance genes are rapidly disseminating between different and novel K. pneumoniae clones which, combined with resistance to multiple antibiotics, can derive into more aggressive and pathogenic multidrug-resistant strains of clinical importance. Our results stress the importance of continuous surveillance of CR Enterobacterales in health facilities so that novel STs carrying resistance and virulence genes that may become increasingly pathogenic can be identified and adequate therapies to adopted to avoid their dissemination and derived pathologies.
DOI: 10.5281/zenodo.6222680
title: Raw Data for the article: HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma

description: Since its identification, HCV has been considered one of the main causes of hepatitis and liver cancer. Currently, the molecular mechanisms of HCC development induced by HCV infection have not been sufficiently clarified. The recent discovery of novel treatments that inhibit HCV replication gave rise to new questions concerning HCC mechanisms. In particular, the HCV eradication mediated by new direct-acting antiviral (DAAs) drugs does not exclude the possibility of <em>de novo</em> HCC development; this finding opened more questions on the interplay between liver cells and the virus. Different groups have investigated the pathways leading to cancer recurrence in patients treated with DAAs. For this reason, we tried to gain molecular insights into the changes induced by HCV infection in the target liver cells. In particular, we observed an increase in microRNA34a (miR34a) expression following HCV infection of HCC cell line Huh7.5. In addition, Huh7.5 treated with extracellular vesicles (EVs) from the previously HCV-infected Huh7.5 underwent apoptosis. Since miR34 expression was increased in Huh7.5 EVs, we hypothesized a paracrine mechanism of viral infection mediated by miR34a cargo of EVs. The balance between viral infection and cell transformation may raise some questions on the possible use of antiviral drugs in association with antineoplastic treatment.
DOI: 10.5281/zenodo.6222679
title: Raw Data for the article: HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma

description: Since its identification, HCV has been considered one of the main causes of hepatitis and liver cancer. Currently, the molecular mechanisms of HCC development induced by HCV infection have not been sufficiently clarified. The recent discovery of novel treatments that inhibit HCV replication gave rise to new questions concerning HCC mechanisms. In particular, the HCV eradication mediated by new direct-acting antiviral (DAAs) drugs does not exclude the possibility of <em>de novo</em> HCC development; this finding opened more questions on the interplay between liver cells and the virus. Different groups have investigated the pathways leading to cancer recurrence in patients treated with DAAs. For this reason, we tried to gain molecular insights into the changes induced by HCV infection in the target liver cells. In particular, we observed an increase in microRNA34a (miR34a) expression following HCV infection of HCC cell line Huh7.5. In addition, Huh7.5 treated with extracellular vesicles (EVs) from the previously HCV-infected Huh7.5 underwent apoptosis. Since miR34 expression was increased in Huh7.5 EVs, we hypothesized a paracrine mechanism of viral infection mediated by miR34a cargo of EVs. The balance between viral infection and cell transformation may raise some questions on the possible use of antiviral drugs in association with antineoplastic treatment.
DOI: 10.6084/m9.figshare.17696738
title: Figshare file 1: LISTADAPT Complete dataset, epidemiological informations, genomic quality assessment data and raw reads accession number

description: This table provides, for the 1484 LISTADAPT dataset, all information available dealing with epidemiological data and genome sequence quality.
DOI: 10.6084/m9.figshare.17696738.v2
title: Figshare file 1: LISTADAPT Complete dataset, epidemiological informations, genomic quality assessment data and raw reads accession number

description: This table provides, for the 1484 LISTADAPT dataset, all information available dealing with epidemiological data and genome sequence quality.
DOI: 10.6084/m9.figshare.17696738.v1
title: Figshare file 1: LISTADAPT Complete dataset, epidemiological informations, genomic quality assessment data and raw reads accession number

description: This table provides, for the 1485 LISTADAPT dataset, all information available dealing with epidemiological data and genome sequence quality.
DOI: 10.5281/zenodo.6221884
title: Raw Data for the article: A Radioactive-Free Method for the Thorough Analysis of the Kinetics of Cell Cytotoxicity

description: The cytotoxic activity of T cells and Natural Killer cells is usually measured with the chromium release assay (CRA), which involves the use of 51Chromium (<sup>51</sup>Cr), a radioactive substance dangerous to the operator and expensive to handle and dismiss. The accuracy of the measurements depends on how well the target cells incorporate <sup>51</sup>Cr during labelling which, in turn, depends on cellular division. Due to bystander metabolism, the target cells spontaneously release <sup>51</sup>Cr, producing a high background noise. Alternative radioactive-free methods have been developed. Here, we compare a bioluminescence (BLI)-based and a carboxyfluorescein succinimidyl ester (CFSE)-based cytotoxicity assay to the standard radioactive CRA. In the first assay, the target cells stably express the enzyme luciferase, and vitality is measured by photon emission upon the addition of the substrate d-luciferin. In the second one, the target cells are labelled with CFSE, and the signal is detected by Flow Cytometry. We used these two protocols to measure cytotoxicity induced by treatment with NK cells. The cytotoxicity of NK cells was determined by adding increasing doses of human NK cells. The results obtained with the BLI method were consistent with those obtained with the CRA- or CFSE-based assays 4 hours after adding the NK cells. Most importantly, with the BLI assay, the kinetic of NK cells' killing was thoroughly traced with multiple time point measurements, in contrast with the single time point measurement the other two methods allow, which unveiled additional information on NK cell killing pathways.
DOI: 10.5281/zenodo.6221883
title: Raw Data for the article: A Radioactive-Free Method for the Thorough Analysis of the Kinetics of Cell Cytotoxicity

description: The cytotoxic activity of T cells and Natural Killer cells is usually measured with the chromium release assay (CRA), which involves the use of 51Chromium (<sup>51</sup>Cr), a radioactive substance dangerous to the operator and expensive to handle and dismiss. The accuracy of the measurements depends on how well the target cells incorporate <sup>51</sup>Cr during labelling which, in turn, depends on cellular division. Due to bystander metabolism, the target cells spontaneously release <sup>51</sup>Cr, producing a high background noise. Alternative radioactive-free methods have been developed. Here, we compare a bioluminescence (BLI)-based and a carboxyfluorescein succinimidyl ester (CFSE)-based cytotoxicity assay to the standard radioactive CRA. In the first assay, the target cells stably express the enzyme luciferase, and vitality is measured by photon emission upon the addition of the substrate d-luciferin. In the second one, the target cells are labelled with CFSE, and the signal is detected by Flow Cytometry. We used these two protocols to measure cytotoxicity induced by treatment with NK cells. The cytotoxicity of NK cells was determined by adding increasing doses of human NK cells. The results obtained with the BLI method were consistent with those obtained with the CRA- or CFSE-based assays 4 hours after adding the NK cells. Most importantly, with the BLI assay, the kinetic of NK cells' killing was thoroughly traced with multiple time point measurements, in contrast with the single time point measurement the other two methods allow, which unveiled additional information on NK cell killing pathways.
DOI: 10.5281/zenodo.6258750
title: Bayesian Online Learning for Energy-Aware Resource Orchestration in Virtualized RANs - Dataset

description: Dataset providing a set of measurement of performance and power consumpetion of a virtualized Base Station (srseNB).
DOI: 10.5281/zenodo.6258749
title: Bayesian Online Learning for Energy-Aware Resource Orchestration in Virtualized RANs - Dataset

description: Dataset providing a set of measurement of performance and power consumpetion of a virtualized Base Station (srseNB).
DOI: 10.6073/pasta/fb5e010a3423b601b898b88f10fe46a2
title: Vegetation profile and canopy height of Tabonuco, Colorado and Cloud Forests

description: The overall height and the presence of vegetation in different height intervals above ground is recorded above points on a 5 x 5 m grid in three hectare-sized plots in the Luquillo Experimental Forest, Puerto Rico. One plots each is in tabonuco forest (350 m elevation), colorado forest (650 m) and cloud forest (1000 m). The first records were made shortly before Hurricane Hugo, in 1989. Post hurricane measurements have been made five time to six time in each plot, the most recent been in 1998 for all plots. The results show the damage to forest structure wreaked by Hurricane Hugo, steady recovery of forest structure after Hugo, minor damage by Hurricane Hortense in 1996, and resumption of recovery after Hortense. Maximum canopy height and the vertical distribution of vegetation in the forests are returning to pre-hurricane Hugo values.
DOI: 10.5281/zenodo.6221703
title: Raw Data for the article: Post-crisis debriefing: A tool for improving quality in the medical emergency team system

description: <strong>Objectives: </strong>To examine clinicians' perception of quality of technical and non-technical response to emergencies and application of post crisis debriefing. <strong>Design: </strong>Descriptive, anonymous, self-reporting survey on the needs and perception of a post-crisis debriefing implementation. <strong>Setting: </strong>Multi-specialist medical institute in Italy focused on solid organ transplantation and organ failure support. <strong>Main outcomes: </strong>Perception of application of guidelines and evaluation of debriefing implementation during in-hospital emergencies. <strong>Results: </strong>Response rate to the survey was 25% (148 health care workers). Of all respondents, 86% were employed &gt;10 years, 75% were involved in ≤5 emergencies over the previous year. Resuscitation guidelines were considered fully applied by 55%; 64% of respondents considered the teaching programme as sufficient. Of all participants, 97% were aware of the importance of teamwork dynamics, 79% were aware of the importance of the personal performance, and 52% considered emergencies as valid opportunities for professional growth. Leadership was considered important by 45% of respondents; debriefing implemented by 41%, and considered a potentially useful tool by 85%. <strong>Conclusion: </strong>Post-crisis debriefing is a way to diffuse self-reflective and life-long learning culture; it is perceived as a powerful tool for improving quality of the rapid response system by the vast majority of those surveyed. <strong>Keywords: </strong>Communication; Crisis resource management; Emergency; ICU; Intensive care medicine; Leadership; Patient safety; Teamwork.
DOI: 10.5281/zenodo.6221702
title: Raw Data for the article: Post-crisis debriefing: A tool for improving quality in the medical emergency team system

description: <strong>Objectives: </strong>To examine clinicians' perception of quality of technical and non-technical response to emergencies and application of post crisis debriefing. <strong>Design: </strong>Descriptive, anonymous, self-reporting survey on the needs and perception of a post-crisis debriefing implementation. <strong>Setting: </strong>Multi-specialist medical institute in Italy focused on solid organ transplantation and organ failure support. <strong>Main outcomes: </strong>Perception of application of guidelines and evaluation of debriefing implementation during in-hospital emergencies. <strong>Results: </strong>Response rate to the survey was 25% (148 health care workers). Of all respondents, 86% were employed &gt;10 years, 75% were involved in ≤5 emergencies over the previous year. Resuscitation guidelines were considered fully applied by 55%; 64% of respondents considered the teaching programme as sufficient. Of all participants, 97% were aware of the importance of teamwork dynamics, 79% were aware of the importance of the personal performance, and 52% considered emergencies as valid opportunities for professional growth. Leadership was considered important by 45% of respondents; debriefing implemented by 41%, and considered a potentially useful tool by 85%. <strong>Conclusion: </strong>Post-crisis debriefing is a way to diffuse self-reflective and life-long learning culture; it is perceived as a powerful tool for improving quality of the rapid response system by the vast majority of those surveyed. <strong>Keywords: </strong>Communication; Crisis resource management; Emergency; ICU; Intensive care medicine; Leadership; Patient safety; Teamwork.
DOI: 10.5281/zenodo.6221339
title: Raw Data for the article: miRNA expression analysis in the human heart: Undifferentiated progenitors vs. bioptic tissues-Implications for proliferation and ageing

description: In developed countries, cardiovascular diseases are currently the first cause of death. Cardiospheres (CSs) and cardiosphere-derived cells (CDCs) have been found to have the ability to regenerate the myocardium after myocardial infarction (MI). In recent years, much effort has been made to gain insight into the human heart repair mechanisms, in which miRNAs have been shown to play an important role. In this regard, to elucidate the involvement of miRNAs, we evaluated the miRNA expression profile across human heart biopsy, CSs and CDCs using microarray and next-generation sequencing (NGS) technologies. We identified several miRNAs more represented in the progenitors, where some of them can be responsible for the proliferation or the maintenance of an undifferentiated state, while others have been found to be downregulated in the undifferentiated progenitors compared with the biopsies. Moreover, we also found a correlation between downregulated miRNAs in CSs/CDCs and patient age (eg miR-490) and an inverse correlation among miRNAs upregulated in CSs/CDCs (eg miR-31).
DOI: 10.5281/zenodo.6221338
title: Raw Data for the article: miRNA expression analysis in the human heart: Undifferentiated progenitors vs. bioptic tissues-Implications for proliferation and ageing

description: In developed countries, cardiovascular diseases are currently the first cause of death. Cardiospheres (CSs) and cardiosphere-derived cells (CDCs) have been found to have the ability to regenerate the myocardium after myocardial infarction (MI). In recent years, much effort has been made to gain insight into the human heart repair mechanisms, in which miRNAs have been shown to play an important role. In this regard, to elucidate the involvement of miRNAs, we evaluated the miRNA expression profile across human heart biopsy, CSs and CDCs using microarray and next-generation sequencing (NGS) technologies. We identified several miRNAs more represented in the progenitors, where some of them can be responsible for the proliferation or the maintenance of an undifferentiated state, while others have been found to be downregulated in the undifferentiated progenitors compared with the biopsies. Moreover, we also found a correlation between downregulated miRNAs in CSs/CDCs and patient age (eg miR-490) and an inverse correlation among miRNAs upregulated in CSs/CDCs (eg miR-31).
DOI: 10.5281/zenodo.4617663
title: Raw Data for the article: Radiation exposure during transjugular intrahepatic portosystemic shunt creation in patients with complete portal vein thrombosis or portal cavernoma

description: <strong>Background: </strong>This study aims to evaluate radiation exposure in patients with complete portal vein thrombosis (CPVT) or portal cavernoma (PC) undergoing transjugular intrahepatic portosystemic shunt (TIPS) creation using real-time ultrasound guidance for portal vein targeting. <strong>Materials and methods: </strong>This is a single institution retrospective analysis. Between August 2009 and September 2018, TIPS was attempted in 49 patients with CPVT or PC. Radiation exposure (dose area product [DAP], air KERMA (AK) and fluoroscopy time [FT]), technical success, clinical success, complications and survival were analyzed. <strong>Results: </strong>In total, 29 patients had CPVT and 20 patients had PC. 41/49 patients had cirrhosis. TIPS indications were refractory ascites (n = 25), variceal bleeding (n = 16) and other (n = 8). TIPS was successfully placed in 94% (46/49) of patients via a transjugular approach alone (n = 40), a transjugular/transhepatic approach (n = 5) and a transjugular/transsplenic approach (n = 1). Median DAP was 261 Gy * cm<sup>2</sup> (range 29-950), median AK was 0.2 Gy (range 0.05-0.5), and median FT was 28.2 min (range 7.7-93.7). Mean portosystemic pressure gradient decreased from 16.8 ± 5.1 mmHg to 7.5 ± 3.3 mmHg (P &lt; 0.01). There were no major procedural complications. Overall clinical success was achieved in 77% of patients (mean follow-up of 21.1 months). Encephalopathy was observed in 16 patients (34%), grade II-III encephalopathy in 7 patients (15%). TIPS revision was performed in 15 patients (32%). Overall survival rate was 75%. <strong>Conclusion: </strong>In our experience, the use of real-time ultrasound guidance allowed the majority of the TIPS to be performed via a transjugular approach alone with a reasonably low radiation exposure considering the high technical difficulties of the selected cohort of patients with CVPT or PC.
DOI: 10.5281/zenodo.4621088
title: Raw Data for the article: Feasibility of combined ECG-Gated and Helical acquisition mode in a pre-TAVI computed tomography angiography protocol using a fixed low-volume contrast medium injection

description: <strong>Background: </strong>To investigate the feasibility, image quality, and clinical implications of a combined ECG-gated and helical acquisition mode in a computed tomography angiography (CTA) protocol in patients scheduled for transcatheter aortic valve implantation (TAVI) using a fixed, low-volume, contrast medium injection. <strong>Methods: </strong>Between July and October 2019, 43 TAVI candidates underwent investigation with CTA using a 64-slice CT scanner. Images obtained were prospectively evaluated. 65 mL of low iodine dose contrast medium (CM), followed by 25 mL of saline, were administered using a fixed multiphasic injection protocol in all patients. Patients were divided into three groups based on BMI: Group 1 (n = 9) with BMI &lt; 22 kg/m<sup>2</sup>; Group 2 (n = 22) with BMI 22-29 kg/m<sup>2</sup>; Group 3 (n = 12) with BMI &gt; 29 kg/m<sup>2</sup>. Images were evaluated for image quality, vessel attenuation (HU), Signal-to-Noise Ratio (SNR), Contrast-to-Noise Ratio (CNR) and estimated radiation dose. Image quality of the aortic root and iliac-femoral vessels was diagnostic in all patients. <strong>Results: </strong>Vascular attenuation was &gt; 200 HU and CNR &gt; 3 at all vessel levels. <strong>Conclusion: </strong>Data from our study suggest that it is possible to image the aortic annulus and aorto-iliac anatomy and obtain high image quality in all patients by using a combined ECG-gated and helical acquisition mode in a computed tomography angiography (CTA) protocol with a fixed low-volume contrast medium injection (65 mL). This allows for accurate CT measurements of the aortic annulus, recruitment of patients for TAVI and facilitates pre-procedural planning in these high surgical risk patients.
DOI: 10.5281/zenodo.4621087
title: Raw Data for the article: Feasibility of combined ECG-Gated and Helical acquisition mode in a pre-TAVI computed tomography angiography protocol using a fixed low-volume contrast medium injection

description: <strong>Background: </strong>To investigate the feasibility, image quality, and clinical implications of a combined ECG-gated and helical acquisition mode in a computed tomography angiography (CTA) protocol in patients scheduled for transcatheter aortic valve implantation (TAVI) using a fixed, low-volume, contrast medium injection. <strong>Methods: </strong>Between July and October 2019, 43 TAVI candidates underwent investigation with CTA using a 64-slice CT scanner. Images obtained were prospectively evaluated. 65 mL of low iodine dose contrast medium (CM), followed by 25 mL of saline, were administered using a fixed multiphasic injection protocol in all patients. Patients were divided into three groups based on BMI: Group 1 (n = 9) with BMI &lt; 22 kg/m<sup>2</sup>; Group 2 (n = 22) with BMI 22-29 kg/m<sup>2</sup>; Group 3 (n = 12) with BMI &gt; 29 kg/m<sup>2</sup>. Images were evaluated for image quality, vessel attenuation (HU), Signal-to-Noise Ratio (SNR), Contrast-to-Noise Ratio (CNR) and estimated radiation dose. Image quality of the aortic root and iliac-femoral vessels was diagnostic in all patients. <strong>Results: </strong>Vascular attenuation was &gt; 200 HU and CNR &gt; 3 at all vessel levels. <strong>Conclusion: </strong>Data from our study suggest that it is possible to image the aortic annulus and aorto-iliac anatomy and obtain high image quality in all patients by using a combined ECG-gated and helical acquisition mode in a computed tomography angiography (CTA) protocol with a fixed low-volume contrast medium injection (65 mL). This allows for accurate CT measurements of the aortic annulus, recruitment of patients for TAVI and facilitates pre-procedural planning in these high surgical risk patients.