DOI: 10.5061/dryad.gxd2547t1
title: Oophaga sylvatica decahydroquinoline quantification

description: Shifts in microbiome community composition can have large effects on host
 health. It is therefore important to understand how perturbations, like
 those caused by the introduction of exogenous chemicals, modulate
 microbiome community composition. In poison frogs within the family
 Dendrobatidae, the skin microbiome is exposed to the alkaloids that the
 frogs sequester from their diet and use for defense. Given the
 demonstrated antimicrobial effects of these poison frog alkaloids, these
 compounds may be structuring the skin microbial community. To test this,
 we first characterized microbial communities from chemically defended and
 closely related non-defended frogs from Ecuador. Then we conducted a
 laboratory experiment to monitor the effect of the alkaloid
 decahydroquinoline (DHQ) on the microbiome of a single frog species. In
 both the field and lab experiments, we found that alkaloid-exposed
 microbiomes are more species rich and phylogenetically diverse, with an
 increase in rare taxa. To better understand the strain-specific behavior
 in response to alkaloids, we cultured microbial strains from poison frog
 skin and found the majority of strains exhibited either enhanced growth or
 were not impacted by the addition of DHQ. Additionally, stable isotope
 tracing coupled to nanoSIMS suggests that some of these strains are able
 to metabolize DHQ. Taken together, these data suggest that poison frog
 chemical defenses open new niches for skin-associated microbes with
 specific adaptations, including the likely metabolism of alkaloids, that
 enable their survival in this toxic environment. This work helps expand
 our understanding of how exposure to exogenous compounds like alkaloids
 can impact host microbiomes.  Other data related to this
 manuscript can be found in the following locations:
 https://doi.org/10.5061/dryad.5qfttdzd5, Wild dendrobatid frog microbiomes
 - bacteriahttps://doi.org/10.5061/dryad.gxd2547t1, Oophaga sylvatica
 decahydroquinoline quantificationhttps://doi.org/10.5061/dryad.9ghx3ffqn,
 Ecuadorian Dendrobatid frog alkaloid
 profileshttps://doi.org/10.5061/dryad.tdz08kq62, Feeding experiment
 microbiome - bacteriahttps://doi.org/10.5061/dryad.4mw6m90hn, Feeding
 experiment microbiome - fungihttps://doi.org/10.5061/dryad.2ngf1vhzc,
 Poison frog microbiome metagenomics
 datahttps://doi.org/10.5061/dryad.9cnp5hqrm, Wild dendrobatid microbiomes
 – fungi
description: # Oophaga sylvatica decahydroquinoline quantification
 [https://doi.org/10.5061/dryad.gxd2547t1](https://doi.org/10.5061/dryad.gxd2547t1) This Liquid Chromatography Mass Spectrometry (LCMS) data was generated in a controlled feeding experiment to test for the role of the alkaloid decahydroquinoline (DHQ) in shaping the skin microbiome of the frogs Oophaga sylvatica and Allobates femoralis. The quantity of DHQ was measured at the end of the experiment. Files are raw data from a Waters Acquity UPLC and Thermo Exploris 240 BioPharma orbitrap mass spectrometer. Files include mass to charge ratios, retention times, and intensities. Data files can also be opened with various open source software, including OpenChrom ([https://www.openchrom.net/](https://www.openchrom.net/)).  Other data related to this manuscript can be found in the following locations: [https://doi.org/10.5061/dryad.5qfttdzd5](https://doi.org/10.5061/dryad.5qfttdzd5), Wild dendrobatid frog microbiomes - bacteria [https://doi.org/10.5061/dryad.gxd2547t1](https://doi.org/10.5061/dryad.gxd2547t1), Oophaga sylvatica decahydroquinoline quantification [https://doi.org/10.5061/dryad.9ghx3ffqn](https://doi.org/10.5061/dryad.9ghx3ffqn), Ecuadorian Dendrobatid frog alkaloid profiles [https://doi.org/10.5061/dryad.tdz08kq62](https://doi.org/10.5061/dryad.tdz08kq62), Feeding experiment microbiome - bacteria [https://doi.org/10.5061/dryad.4mw6m90hn](https://doi.org/10.5061/dryad.4mw6m90hn), Feeding experiment microbiome - fungi [https://doi.org/10.5061/dryad.2ngf1vhzc](https://doi.org/10.5061/dryad.2ngf1vhzc), Poison frog microbiome metagenomics data [https://doi.org/10.5061/dryad.9cnp5hqrm](https://doi.org/10.5061/dryad.9cnp5hqrm), Wild dendrobatid microbiomes – fungi ## Sample Metadata See details in file `Feeding_experiment_alkaloids_metadata.xlsx` * **Sample Name:** name of corresponding data file * **Sample Type:** Frog, standard curve sample, or blank * **Frog Number:** Corresponds to frog number in other feeding experiment data * **Frog Species:** Either OS (Oophaga sylvatica) or AF (Allobates femoralis) * **Treatment:** Frogs either received DHQ (toxic) or a vehicle control (non-toxic) in the feeding experiment.  Cells are blank where the columns are not applicable to the sample.