cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6504728
title: Residual polysaccharides from fungi reduce the bacterial spot in tomato plants

description: Abstract Polysaccharides from fungal wastes were partially characterized and evaluated for their protective effects against bacterial spot caused by Xanthomonas gardneri on four tomato cultivars: Santa Cruz Kada, Natália, BRS Sena and Forty. The polysaccharides were extracted from spent mushroom substrate of Pleurotus ostreatus, residual brewery yeast (Saccharomyces cerevisiae), and basidiocarps discarded from Lentinula edodes production. These polysaccharides were characterized for total carbohydrates, phenolics and proteins content, pH, scatter intensity, conductivity, Zeta potential, DPPH scavenging assay and infrared spectroscopy. The effects of time interval between treatment and inoculation (4 or 7 days) and polysaccharide concentrations (0.5 or 1.5 mg.mL–1) were assessed for disease severity using a susceptible tomato cultivar. The polysaccharide action mode was investigated by determining the activity of peroxidases and phenylalanine ammonialyase and by quantifying flavonoids and total phenolics in the plants treated and challenged with X. gardneri. The polysaccharides obtained from Lentinula edodes (PSHII), Saccharomyces cerevisiae (PRC) and Pleurotus ostreatus (PSPO) (1.5 mg.mL-1) reduced bacterial spot severity by 50% on tomato cotyledons, leaflets and five-leaf plants. Furthermore, PRC and PSHII (1.5 mg.mL–1) could decrease disease severity in all tested cultivars. PSHII, the most effective, did not cause change in phenylalanine ammonia-lyase activity or flavonoid content on the cultivars Kada and Natália. However, an increase in peroxidase activity and total phenol content on cv. Kada was noted. The polysaccharides obtained from food industry wastes could provide protection against bacterial spot on tomato cultivars by inducing defense mechanisms and can be useful in formulating products with phytosanitary potential.
DOI: 10.6084/m9.figshare.6504728.v1
title: Residual polysaccharides from fungi reduce the bacterial spot in tomato plants

description: Abstract Polysaccharides from fungal wastes were partially characterized and evaluated for their protective effects against bacterial spot caused by Xanthomonas gardneri on four tomato cultivars: Santa Cruz Kada, Natália, BRS Sena and Forty. The polysaccharides were extracted from spent mushroom substrate of Pleurotus ostreatus, residual brewery yeast (Saccharomyces cerevisiae), and basidiocarps discarded from Lentinula edodes production. These polysaccharides were characterized for total carbohydrates, phenolics and proteins content, pH, scatter intensity, conductivity, Zeta potential, DPPH scavenging assay and infrared spectroscopy. The effects of time interval between treatment and inoculation (4 or 7 days) and polysaccharide concentrations (0.5 or 1.5 mg.mL–1) were assessed for disease severity using a susceptible tomato cultivar. The polysaccharide action mode was investigated by determining the activity of peroxidases and phenylalanine ammonialyase and by quantifying flavonoids and total phenolics in the plants treated and challenged with X. gardneri. The polysaccharides obtained from Lentinula edodes (PSHII), Saccharomyces cerevisiae (PRC) and Pleurotus ostreatus (PSPO) (1.5 mg.mL-1) reduced bacterial spot severity by 50% on tomato cotyledons, leaflets and five-leaf plants. Furthermore, PRC and PSHII (1.5 mg.mL–1) could decrease disease severity in all tested cultivars. PSHII, the most effective, did not cause change in phenylalanine ammonia-lyase activity or flavonoid content on the cultivars Kada and Natália. However, an increase in peroxidase activity and total phenol content on cv. Kada was noted. The polysaccharides obtained from food industry wastes could provide protection against bacterial spot on tomato cultivars by inducing defense mechanisms and can be useful in formulating products with phytosanitary potential.
DOI: 10.6084/m9.figshare.6504725
title: Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study

description: Abstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p &lt; 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes – chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor – in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization.
DOI: 10.6084/m9.figshare.6504725.v1
title: Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study

description: Abstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p &lt; 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes – chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor – in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization.
DOI: 10.6084/m9.figshare.6504713
title: Agreement between the nutritional status of schoolchildren and the perception of their guardians

description: ABSTRACT Objective Assess the correlation between classification of nutritional status of schoolchildren and the perception of their guardians. Methods A cross-sectional study with children aged 6-10 years from a public school in the South of Brazil. Weight and height of the schoolchildren were collected, and the guardians answered a questionnaire containing the range of silhouettes of Brazilian children. The perception through the scale of silhouettes was compared with the actual nutritional status. Kappa coefficient was used to check the consistency of actual and perceived perception. The prevalence ratio was calculated between perceived overweight and well-nourished children. Results A total of 236 guardians of schoolchildren were interviewed. The level of agreement between the nutritional status assessment of actual and perceived perception was weak (Kappa=0.32, p&lt;0.001). As for the perception of nutritional status, 50.8% of the guardians scored the nutritional rating their children correctly, of which 42.0% of these children were overweight and 60.2% were well-nourished, p&lt;0.05. The prevalence of guardians who underestimated the classification of the nutritional status was 21.6%, and underestimation of overweight children was 1.8 times greater than of well-nourished children. The overestimation of the nutritional status was 27.5%. The most prevalent nutritional status was well-nourished, followed by overweight. Conclusion The study showed that guardians do not have the correct perception of the nutritional status of the schoolchildren. Public interventions for health education must be conducted to reduce erroneous perceptions.
DOI: 10.6084/m9.figshare.6504713.v1
title: Agreement between the nutritional status of schoolchildren and the perception of their guardians

description: ABSTRACT Objective Assess the correlation between classification of nutritional status of schoolchildren and the perception of their guardians. Methods A cross-sectional study with children aged 6-10 years from a public school in the South of Brazil. Weight and height of the schoolchildren were collected, and the guardians answered a questionnaire containing the range of silhouettes of Brazilian children. The perception through the scale of silhouettes was compared with the actual nutritional status. Kappa coefficient was used to check the consistency of actual and perceived perception. The prevalence ratio was calculated between perceived overweight and well-nourished children. Results A total of 236 guardians of schoolchildren were interviewed. The level of agreement between the nutritional status assessment of actual and perceived perception was weak (Kappa=0.32, p&lt;0.001). As for the perception of nutritional status, 50.8% of the guardians scored the nutritional rating their children correctly, of which 42.0% of these children were overweight and 60.2% were well-nourished, p&lt;0.05. The prevalence of guardians who underestimated the classification of the nutritional status was 21.6%, and underestimation of overweight children was 1.8 times greater than of well-nourished children. The overestimation of the nutritional status was 27.5%. The most prevalent nutritional status was well-nourished, followed by overweight. Conclusion The study showed that guardians do not have the correct perception of the nutritional status of the schoolchildren. Public interventions for health education must be conducted to reduce erroneous perceptions.
DOI: 10.6084/m9.figshare.6504710
title: Near-IR spectroscopy predictions can determine the digestibility and feed intake from feedlot lambs

description: ABSTRACT This research was carried out to validate the effectiveness of the Near-IR spectroscopy method in predicting intake and digestibility of DM and nutrients of diets for feedlot lambs, in order to compare with the conventional laboratory method. Samples of diets, ort,s and feces of forty Santa Ines lambs were subjected to both methods and analyzed for DM, MM, NDF, ADF, CP, and EE for future calculations for intake and digestibility. Most of the model's correlation coefficient was in the range of 0.66 to 0.81, being suitable for quantitative prediction. Highest values for CP content of 0.85 (diets) and 0.87 (orts) were observed. There were high correlations between the methods to predicting intake (average values of 0.93) and digestibility (average values of 0.93). Correlations between the variables obtained by the prediction of NIRS and the conventional method were adequate, showing that regardless of the method, the nutritional content of diets, orts and feces can be used to calculate the intake and digestibility of DM and nutrients of lambs’ diets. The Near-IR spectroscopy method is recommended for accuracy, speed and low cost compared to conventional method.
DOI: 10.6084/m9.figshare.6504710.v1
title: Near-IR spectroscopy predictions can determine the digestibility and feed intake from feedlot lambs

description: ABSTRACT This research was carried out to validate the effectiveness of the Near-IR spectroscopy method in predicting intake and digestibility of DM and nutrients of diets for feedlot lambs, in order to compare with the conventional laboratory method. Samples of diets, ort,s and feces of forty Santa Ines lambs were subjected to both methods and analyzed for DM, MM, NDF, ADF, CP, and EE for future calculations for intake and digestibility. Most of the model's correlation coefficient was in the range of 0.66 to 0.81, being suitable for quantitative prediction. Highest values for CP content of 0.85 (diets) and 0.87 (orts) were observed. There were high correlations between the methods to predicting intake (average values of 0.93) and digestibility (average values of 0.93). Correlations between the variables obtained by the prediction of NIRS and the conventional method were adequate, showing that regardless of the method, the nutritional content of diets, orts and feces can be used to calculate the intake and digestibility of DM and nutrients of lambs’ diets. The Near-IR spectroscopy method is recommended for accuracy, speed and low cost compared to conventional method.
DOI: 10.6084/m9.figshare.6504707
title: Statistical methods to study adaptability and stability in breeding lines of food-type soybeans

description: ABSTRACT The grains of food-type soybean cultivars, which are characterized by the absence of lipoxygenases and the presence of high levels of proteins and isoflavones, are regarded as functional foods with high acceptance by consumers. However, few cultivars of food-type soybeans are currently available in the Brazilian market. The aim of this work was to study the adaptability and stability of various genotypes of food-type soybeans and to compare the performance of methods, which are based on analysis of variance, non-parametric, regression, multivariate and mixed models. Ten lines of food-type soybeans obtained from the Breeding Program of Soybeans for Human Consumption of the State University of Londrina (UEL/BPSHC) and two commercial varieties, the foodtype cultivar BRS 257 and the cultivar BMX Potência RR, were evaluated in the counties of Londrina, Guarapuava, Ponta Grossa and Pato Branco, Paraná, Brazil, during the two sowing seasons of the harvest of 2014/2015. The characteristic evaluated was grain yield. The adaptability parameters of Eberhart and Russel and Cruz methods showed high correlations with the Wricke model. The parameters provided by the analyses of Lins and Binns and REML/BLUP showed higher grain yield associations and moderated correlations with the Eskridge parameters. The AMMI offered the possibility of use in conjunction with the other methodologies. When yield, adaptability and stability were considered, the genotypes UEL 110, UEL 122, UEL 121 and UEL 123 demonstrated potential for the development of new cultivars of food-type soybeans in which lipoxygenases are absent.
DOI: 10.6084/m9.figshare.6504707.v1
title: Statistical methods to study adaptability and stability in breeding lines of food-type soybeans

description: ABSTRACT The grains of food-type soybean cultivars, which are characterized by the absence of lipoxygenases and the presence of high levels of proteins and isoflavones, are regarded as functional foods with high acceptance by consumers. However, few cultivars of food-type soybeans are currently available in the Brazilian market. The aim of this work was to study the adaptability and stability of various genotypes of food-type soybeans and to compare the performance of methods, which are based on analysis of variance, non-parametric, regression, multivariate and mixed models. Ten lines of food-type soybeans obtained from the Breeding Program of Soybeans for Human Consumption of the State University of Londrina (UEL/BPSHC) and two commercial varieties, the foodtype cultivar BRS 257 and the cultivar BMX Potência RR, were evaluated in the counties of Londrina, Guarapuava, Ponta Grossa and Pato Branco, Paraná, Brazil, during the two sowing seasons of the harvest of 2014/2015. The characteristic evaluated was grain yield. The adaptability parameters of Eberhart and Russel and Cruz methods showed high correlations with the Wricke model. The parameters provided by the analyses of Lins and Binns and REML/BLUP showed higher grain yield associations and moderated correlations with the Eskridge parameters. The AMMI offered the possibility of use in conjunction with the other methodologies. When yield, adaptability and stability were considered, the genotypes UEL 110, UEL 122, UEL 121 and UEL 123 demonstrated potential for the development of new cultivars of food-type soybeans in which lipoxygenases are absent.
DOI: 10.6084/m9.figshare.6504695
title: Hereditary thrombophilia by factor V Leiden G1691A (heterozygous) and FII prothrombin G20210A (homozygous) mutations in a patient with ischemic cerebrovascular accident

description: ABSTRACT Mutations related to Factor V Leiden (G1691A) and prothrombin (G20210A) are associated with a significantly increased risk of venous thromboembolism. The identification of a patient affected by episodes of venous thromboembolism and carrier of G1691A (heterozygous) and G20210A (homozygous) polymorphisms was determined by molecular tests [real-time polymerase chain reaction (PCR) methodology, Genexpert system] during attendance at the Base Hospital of São José do Rio Preto-SP, Brazil.
DOI: 10.6084/m9.figshare.6504695.v1
title: Hereditary thrombophilia by factor V Leiden G1691A (heterozygous) and FII prothrombin G20210A (homozygous) mutations in a patient with ischemic cerebrovascular accident

description: ABSTRACT Mutations related to Factor V Leiden (G1691A) and prothrombin (G20210A) are associated with a significantly increased risk of venous thromboembolism. The identification of a patient affected by episodes of venous thromboembolism and carrier of G1691A (heterozygous) and G20210A (homozygous) polymorphisms was determined by molecular tests [real-time polymerase chain reaction (PCR) methodology, Genexpert system] during attendance at the Base Hospital of São José do Rio Preto-SP, Brazil.
DOI: 10.6084/m9.figshare.6504692
title: Prevalence and Factors Associated with Metabolic Syndrome among Brazilian Adult Population: National Health Survey - 2013

description: Abstract Background: In Brazil, population-based researches analyzing prevalence and factors associated with metabolic syndrome (MS), a recognized predictor of cardiovascular diseases (CVD), and an important cause of disability and death in the country are scarce. Objective: To evaluate prevalence of MS and its associated factors in Brazilian population. Methods: Secondary analysis of the 2013 National Health Survey, a cross-sectional survey with national representativeness of Brazilian adult population (n = 59,402). MS was the outcome variable, defined from harmonization of cardiology international consensus as load ≥ 3 of the following components: self-reported diabetes and hypercholesterolemia, high blood pressure and high waist circumference. Analysis were stratified by sex and prevalence ratios, with their respective 99% confidence intervals (PR [CI 99%]) calculated by simple and multiple Poisson regression models. Results: MS prevalence was 8.9%, being significantly higher among women compared to men; in general, this pattern was maintained in relation to exposure variables studied. Additionally, less than 25% of population did not present any MS component. In final multiple models, sociodemographic, behavioral and comorbidity variables were associated with MS, however, while low schooling (1.46 [1.23-1.74], cerebrovascular accident (1.36 [1], 00] (1.28 [1.03-1.62]) were associated among women, chronic renal failure (1.85 [2.23-2.76]) was associated exclusively among men. Conclusion: We identified MS high prevalence in Brazilian population; on the other hand, factors associated with this condition were different depending on sex.
DOI: 10.6084/m9.figshare.6504692.v1
title: Prevalence and Factors Associated with Metabolic Syndrome among Brazilian Adult Population: National Health Survey - 2013

description: Abstract Background: In Brazil, population-based researches analyzing prevalence and factors associated with metabolic syndrome (MS), a recognized predictor of cardiovascular diseases (CVD), and an important cause of disability and death in the country are scarce. Objective: To evaluate prevalence of MS and its associated factors in Brazilian population. Methods: Secondary analysis of the 2013 National Health Survey, a cross-sectional survey with national representativeness of Brazilian adult population (n = 59,402). MS was the outcome variable, defined from harmonization of cardiology international consensus as load ≥ 3 of the following components: self-reported diabetes and hypercholesterolemia, high blood pressure and high waist circumference. Analysis were stratified by sex and prevalence ratios, with their respective 99% confidence intervals (PR [CI 99%]) calculated by simple and multiple Poisson regression models. Results: MS prevalence was 8.9%, being significantly higher among women compared to men; in general, this pattern was maintained in relation to exposure variables studied. Additionally, less than 25% of population did not present any MS component. In final multiple models, sociodemographic, behavioral and comorbidity variables were associated with MS, however, while low schooling (1.46 [1.23-1.74], cerebrovascular accident (1.36 [1], 00] (1.28 [1.03-1.62]) were associated among women, chronic renal failure (1.85 [2.23-2.76]) was associated exclusively among men. Conclusion: We identified MS high prevalence in Brazilian population; on the other hand, factors associated with this condition were different depending on sex.
DOI: 10.6084/m9.figshare.6504689
title: Zooplankton community and their relationship with water quality in São Paulo state reservoirs

description: ABSTRACT: Zooplanktonic communities are important components in trophic chain considering primary producers and other levels. In addition, some species are sensitive or tolerant to organic pollution. Here we test the hypothesis that species composition varies according to the eutrophication gradient. We sought to establish which environmental factors are responsible for the variation of the species distribution. This study aimed to characterize zooplankton community and to establish a link between water quality in São Paulo State reservoirs. Samples were collected through vertical hauls with plankton net (68 (m) in seven water bodies. All specimens were identified with specific literature and counted in Sedgwick rafter chamber. Abiotic and biotic data were analyzed by a canonical correspondence analysis. A total of 67 taxa was identified, being Rotifera predominant. The reservoirs exhibited eutrophic conditions mainly Salto Grande, Barra Bonita and Broa. The results point out the rotifers Trichocerca longiseta (Schrank, 1802), Hexarthra sp., Brachiounus spp. and Keratella tropica (Apstein, 1907) followed eutrophication gradient. Additionally, microcrustaceans such as Notodiaptomus, Mesocyclops, Metacyclops and Diaphanossoma occurred in eutrophic reservoirs. On other hand, the cladocerans Ceriodaphnia cornuta (Sars, 1886) and Bosminopsis deitersi Richard, 1895 were considered indicators of better water quality due to relationship with transparency. The results point out that some indicator species are effective in environmental monitoring without reject physico-chemical and biological analysis.