DOI: 10.5061/dryad.dd550
title: Data from: Contemporary pollen-mediated gene immigration reflects the historical isolation of a rare, animal-pollinated shrub in a fragmented landscape

description: Fragmentation is generally considered to have negative impacts on
 widespread outbreeders but impacts on gene flow and diversity in patchy,
 naturally rare, self-compatible plant species remain unclear. We
 investigated diversity, gene flow and contemporary pollen-mediated gene
 immigration in the rare, narrowly distributed endemic shrub Calothamnus
 quadrifidus ssp. teretifolius. This taxon occurs in an internationally
 recognized biodiversity hotspot subjected to recent human-induced
 fragmentation and the condition of the remnants ranges from intact to
 highly degraded. Using microsatellites, we found that inbreeding,
 historically low gene flow and significant population differentiation have
 characterized the genetic system of C. quadrifidus ssp. teretifolius.
 Inbreeding arises from self-pollination, a small amount of biparental
 inbreeding and significant correlation of outcross paternity but fecundity
 was high suggesting populations might have purged their lethals. Paternity
 analyses show that pollinators can move pollen over degraded and intact
 habitat but populations in both intact and degraded remnants had few
 pollen parents per seed parent and low pollen immigration. Genetic
 diversity did not differ significantly between intact and degraded
 remnants but there were signs of genetic bottlenecks and reduced diversity
 in some degraded remnants. Overall, our study suggests human-induced
 fragmentation has not significantly changed the mating system, or pollen
 immigration to, remnant populations and therefore genetic connectivity
 need not be the highest conservation priority. Rather, for rare species
 adapted to higher levels of inbreeding, conservation efforts may be best
 directed to managing intact habitats and ecosystem processes.
description: Calothamnus quadrifidus
 ssp. teretifolius population diversity dataFile containing 160
 Calothamnus quadrifidus subspecies teretifolius individuals sampled from
 eight populations and scored at 9 microsatellite loci. The genotypes are
 in a format suitable for the program GENEPOP 4.1 (Rousset,
 2008).Calothamnus quadrifidus ssp.
 teretifolius.txtT adult
 genotypesFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from T population and scored at 6 microsatellite loci.T progeny
 genotypesFile containing
 Calothamnus quadrifidus subspecies teretifolius progeny in family groups
 sampled from T population and scored at 6 microsatellite loci. Mother
 numbers refer to individuals in the file T adult
 genotypes.txtH adult
 genotypesFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from H population and scored at 6 microsatellite loci.H progeny
 genotypesFile containing
 Calothamnus quadrifidus subspecies teretifolius progeny in family groups
 sampled from H population and scored at 6 microsatellite loci. Mother
 numbers refer to individuals in the file H adult
 genotypes.txtD adult
 genotypesFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from D population and scored at 6 microsatellite loci.D progeny
 genotypesFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from D population and scored at 6 microsatellite loci.B, D, AM
 backgroundFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from B, D, and AM populations and scored at 6 microsatellite
 loci.H, AM, B
 backgroundFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from H, AM, and B populations and scored at 6
 microsatelliteO and N
 backgroundFile containing
 Calothamnus quadrifidus subspecies teretifolius adult individuals sampled
 from O and N populations and scored at 6 microsatellite
 loci.