DOI: 10.5061/dryad.s2t5t
title: Data from: Aging, mortality, and the fast growth trade-off of Schizosaccharomyces pombe

description: Replicative aging has been demonstrated in asymmetrically dividing
 unicellular organisms, seemingly caused by unequal damage partitioning.
 Although asymmetric segregation and inheritance of potential aging factors
 also occurs in symmetrically dividing species, it nevertheless remains
 controversial whether this results in aging. Based on large-scale
 single-cell lineage data obtained by time-lapse microscopy with a
 microfluidic device, in this report, we demonstrate the absence of
 replicative aging in old-pole cell lineages of Schizosaccharomyces pombe
 cultured under constant favorable conditions. By monitoring more than
 1,500 cell lineages in seven different culture conditions, we showed that
 both cell division and death rates are remarkably constant for at least
 50–80 generations. Our measurements revealed that the death rate per
 cellular generation increases with division rate, pointing to a
 physiological trade-off with fast growth under balanced growth conditions.
 We also observed the formation and inheritance of Hsp104-associated
 protein aggregates, which are a potential aging factor in old-pole cell
 lineages, and found that these aggregates exhibited a tendency to
 preferentially remain at the old-poles for several generations. However,
 the aggregates were eventually segregated from old-pole cells upon cell
 division and probabilistically allocated to new-pole cells. We found that
 cell deaths were typically preceded by sudden acceleration of protein
 aggregation, thus relatively large amount of protein aggregates existed at
 the very ends of the dead cell lineages. Our lineage tracking analyses,
 however, revealed that the quantity and inheritance of protein aggregates
 increased neither cellular generation time nor cell death initiation
 rates. Furthermore, our results demonstrated that unusually large amounts
 of protein aggregates induced by oxidative stress exposure did not result
 in aging; old-pole cells resumed normal growth upon stress removal,
 despite the fact that most of them inherited significant quantities of
 aggregates. These results collectively indicate that protein aggregates
 are not a major determinant of triggering cell death in S. pombe, and thus
 cannot be an appropriate molecular marker or index for replicative aging
 under both favorable and stressful environmental conditions.
description: Lineage tracking data
 for YE medium at 28˚CResults_YE_28degree.txt.zipLineage tracking data for YE medium at 30˚CResults_YE_30degree.txt.zipLineage tracking data for YE medium at 34˚CResults_YE_34degree.txt.zipLineage tracking data for EMM medium at 28˚CResults_EMM_28degree.txt.zipLineage tracking data for EMM medium at 30˚CResults_EMM_30degree.txt.zipLineage tracking data for EMM medium at 32˚CResults_EMM_32degree.txt.zipLineage tracking data for EMM medium at 34˚CResults_EMM_34degree.txt.zipLineage and Hsp104-GFP foci tracking data for YE medium at 32˚CResults_YE_32degree_Hsp104_ver2.txtLineage and Hsp104-GFP foci tracking data for YE medium plus H2O2 at 32˚CResults_YE_32degree_H2O2.txtLineage and mNeonGreen-muNS foci tracking data for YE medium at 32˚CResults_YE_32degree_muNS.txtLineage and mNeonGreen-muNs foci tracking data for YE medium at 32˚C in hsp104-deleted strainResults_YE_32degree_muNS_hsp104Deleted.txtDescription of the data structuresDataFilesNumerical values used to produce plots