DOI: 10.5061/dryad.5mkkwh7k7
title: Data from: Ameliorative effect of roxithromycin against prostate inflammation-induced chronic pelvic pain in a preclinical model

description: In view of the well-known anti-inflammatory property of roxithromycin, the
 present study was carried out to evaluate its efficacy in ameliorating
 prostate inflammation-induced chronic pelvic pain in a rat model.
 Inflammation was induced by a single intra-prostatic injection of
 carrageenan, where a time course study revealed a peak histology score on
 day 14. The efficacy study was carried out in four sets of rats, each
 comprising 5 groups, which included the respective normal and experimental
 controls (NC, EC). The drugs (roxithromycin 5 and 20 mg/kg, diclofenac 5
 mg/kg) were given daily orally, and pain score and body weight were
 evaluated at different time intervals. The prostatic index, vascular
 permeability, tissue levels of TNF-α and PGE2, thiobarbituric
 acid reactive substances, glutathione and superoxide dismutase, histology
 score, and immunoreactivity of COX-2 and iNOS were evaluated on day 14.
 Like diclofenac, roxithromycin decreased pain scores, and the rats gained
 weight during the study period as compared to the EC group. The beneficial
 effect of roxithromycin was also evident from the dose-dependent
 restoration of markers of inflammation and oxidative stress.This study
 shows that by alleviating pain and suppressing inflammation and oxidative
 stress,roxithromycin has a potential to provide relief in prostatitis.
description: # Open Source Data We have submitted our raw data. Time_course study
 (**Time_Course.csv**), Body weight and prostatic index
 (**BW_and_PI.csv**), Evans blue dye extraction (**EBD.csv**), Oxidative
 marker GSH (**GSH.csv**), Histology score (**Histology_score.csv**),
 Immunoreactivity score (**IR_SCORE.csv**), Pain_score
 (**Pain_Score.csv**), Inflammatory marker PGE2 (**PGE2.csv**) Oxidative
 marker SOD (**SOD.csv**) Oxidative marker TBARS (**TBARS.csv**),
 Inflammatory marker TNF-α (**TNF-α.csv**) ## Descriptions ### Time_course
 study ``` * Day 0 - Histology score of rats sacrificed on day 0 * Day 7 -
 Histology score of rats sacrificed on day 7 * Day 14 - Histology score of
 rats sacrificed on day 14 * Day 21 - Histology score of rats sacrificed on
 day 21 * n = sample number used for the calculation * Mean & SEM =
 Standard Error of mean ``` ### BW_and_PI * *NC*: Normal control - No
 intervention is done * *EC*: Experimental control - Intra- prostastic
 injection of carrageenan given * *R5*: Intra- prostastic injection of
 carrageenan + Roxithromycin 5 mg/kg * *R20*: Intra- prostastic injection
 of carrageenan + Roxithromycin 20 mg/kg * *D5*: Intra- prostastic
 injection of carrageenan + Diclofenac 5 mg/kg * n = sample number used for
 the calculation * Body weight of rat calculated in grams and prostate
 weight of rat in milligrams * Prostatic Index = prostate weight/Body
 weight - Mean & SEM = Standard Error of mean ### Pain score * *NC*:
 Normal control - No intervention is done * *EC*: Experimental control -
 Intra- prostastic injection of carrageenan given * *R5*: Intra- prostastic
 injection of carrageenan + Roxithromycin 5 mg/kg * *R20*: Intra-
 prostastic injection of carrageenan + Roxithromycin 20 mg/kg * *D5*:
 Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg * n =
 sample number used for the calculation * Mean & SEM = Standard Error
 of mean ### Evans blue dye extravasation * *NC*: Normal control - No
 intervention is done * *EC*: Experimental control - Intra- prostastic
 injection of carrageenan given * *R5*: Intra- prostastic injection of
 carrageenan + Roxithromycin 5 mg/kg * *R20*: Intra- prostastic injection
 of carrageenan + Roxithromycin 20 mg/kg * *D5*: Intra- prostastic
 injection of carrageenan + Diclofenac 5 mg/kg * n = sample number used for
 the calculation * Mean & SEM = Standard Error of mean * Absorbance of
 extracted Evans blue dye is expressed as ng/mg tissue ### Oxidative marker
 GSH * *NC*: Normal control - No intervention is done * *EC*: Experimental
 control - Intra- prostastic injection of carrageenan given * *R5*: Intra-
 prostastic injection of carrageenan + Roxithromycin 5 mg/kg * *R20*:
 Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg *
 *D5*: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg * n
 = sample number used for the calculation * Mean & SEM = Standard Error
 of mean * Tissue levels of GSH is expressed as µg/g tissue ### Oxidative
 marker SOD * *NC*: Normal control - No intervention is done * *EC*:
 Experimental control - Intra- prostastic injection of carrageenan given *
 *R5*: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg *
 *R20*: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
 * *D5*: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg *
 n = sample number used for the calculation * Mean & SEM = Standard
 Error of mean * Absorbance of SOD activity is expressed as U/mg protein
 ### Oxidative marker TBARS * *NC*: Normal control - No intervention is
 done * *EC*: Experimental control - Intra- prostastic injection of
 carrageenan given * *R5*: Intra- prostastic injection of carrageenan +
 Roxithromycin 5 mg/kg * *R20*: Intra- prostastic injection of carrageenan
 + Roxithromycin 20 mg/kg * *D5*: Intra- prostastic injection of
 carrageenan + Diclofenac 5 mg/kg * n = sample number used for the
 calculation * Mean & SEM = Standard Error of mean * Tissue level of
 TBARS is expressed as nmol/g tissue ### Inflammatory marker TNF-α * *NC*:
 Normal control - No intervention is done * *EC*: Experimental control -
 Intra- prostastic injection of carrageenan given * *R5*: Intra- prostastic
 injection of carrageenan + Roxithromycin 5 mg/kg * *R20*: Intra-
 prostastic injection of carrageenan + Roxithromycin 20 mg/kg * *D5*:
 Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg * n =
 sample number used for the calculation * Mean & SEM = Standard Error
 of mean * TNF-α levels were expressed as pg/100 mg tissue ### Inflammatory
 marker PGE2 * *NC*: Normal control - No intervention is done * *EC*:
 Experimental control - Intra- prostastic injection of carrageenan given *
 *R5*: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg *
 *R20*: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
 * *D5*: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg *
 n = sample number used for the calculation * Mean & SEM = Standard
 Error of mean * PGE2 levels were expressed as pg/100 mg tissue ###
 Histology score * *NC*: Normal control - No intervention is done * *EC*:
 Experimental control - Intra- prostastic injection of carrageenan given *
 *R5*: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg *
 *R20*: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
 * *D5*: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg *
 n = sample number used for the calculation * Mean & SEM = Standard
 Error of mean ### Immunoreactivity score * *NC*: Normal control - No
 intervention is done * *EC*: Experimental control - Intra- prostastic
 injection of carrageenan given * *R5*: Intra- prostastic injection of
 carrageenan + Roxithromycin 5 mg/kg * *R20*: Intra- prostastic injection
 of carrageenan + Roxithromycin 20 mg/kg * *D5*: Intra- prostastic
 injection of carrageenan + Diclofenac 5 mg/kg * n = sample number used for
 the calculation * Mean & SEM = Standard Error of mean