cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10258355
title: Bioaccumulation, Growth and Photosynthetic Response of a New Found in Bulgaria Invasive Species Lemna minuta and L. valdiviana to Heavy Metal Pollution

description: ABSTRACT: Heavy metals can meet in the surrounding environment as natural ingredients or from agricultural, industrial and chemical industries. The study was conducted in order to trace the potential of the aquatic plant L. minuta and L. valdiviana for the bioaccumulation of Cu, Cd, and Pb from contaminated water at low levels of these elements. Each of the duckweed species was treated separately with CuSO4.5H2O, CdSO4, Pb SO4 (Valerus, Bulgaria) at 0.5 and 1 mg L-1 concentrations of for 96 hours. After conducting the experiments, relative growth rate (RGR), bioconcentration factor (BCF), tolerant index (Ti) and photosynthetic pigments of two Lemna species were studied. The solution with higher metal concentration more inhibits the growth of macrophytes. The BCF of the metals on the two species were in decreasing order: Cu &gt; Pb &gt; Cd. Our study showed that L. minuta and L. valdiviana at a concentration of 0.5 mg L-1 copper have better affected on the photosynthetic apparatus compared to the control. Better bioaccumulation ability was established in L. minuta compared to L. valdiviana.
DOI: 10.6084/m9.figshare.10258361.v1
title: Interference Relationships Between Weeds and Sugarcane in the ‘Plene’ System

description: ABSTRACT: Sugarcane is one of the most important crops planted in Brazil in that the presence of weeds in the fields has promoted significant yield reduction. The objective of this work was to evaluate the differential growth response of two sugarcane varieties cropped in the ‘Plene’ system as a function of the coexistence with different weed species. Treatments consisted of the coexistence of Bidens pilosa plus Ipomoea quamoclit, Ipomoea hederifolia, Amaranthus viridis, Urochloa plantaginea, Panicum maximum, and Digitaria horizontalis with two sugarcane varieties (RB92579 and SP80-1842) cropped in the ‘Plene’ system, in addition to a control without weeds. The design was completely randomized with three replicates. Dry matter accumulation of weeds was dependent of the weed species and the sugarcane variety whose coexistence was kept. The behavior of development in terms of plant height, number of green leaves, leaf area, and dry mass of stems, roots and leaves was dependent on the sugarcane variety and on the weed species whose coexistence was kept. The weed U. plantaginea showed the greatest potential to interfere with the growth of both sugarcane varieties planted in the ‘Plene’ system. Panicum maximum and D. horizontalis are also potential competitors, while B. pilosa, I. quamoclit, I. hederifolia, and A. viridis are less competitive. The sugarcane variety RB92579 is less susceptible to weed interference than SP80-1842 when planted in the ‘Plene’ system.
DOI: 10.6084/m9.figshare.10258352
title: Cauliflower Cultivars Associated with Urochloa decumbens Straw Mulch in Integrated Weed Management

description: ABSTRACT: In vegetable farming, the reduction in the use of herbicides has been an important factor in the definition of the production system. The objective of this research was to evaluate crop yield of cauliflower cultivars grown on Urochloa decumbens straw mulch, as well as the effect of the association between straw and cultivars on weed management. The treatments were arranged in a 2 x 4 factorial scheme: two managements (with or without U. decumbens straw mulch) and four cauliflower cultivars (Barcelona, Bromus, Júlia and Lisblanc). The experiment used a randomized block design with four replicates. There was no interference of straw mulch on crop yield of cauliflower cultivars. Hybrids Barcelona and Júlia presented yields of 22.0 and 23.9 t ha-1, respectively, and they did not differ from each other. The Bromus and Lisblanc materials did not adapt to the edaphoclimatic conditions of the region, which resulted in reduced cultural control and high weed emergence after weeding. U. decumbens straw had high control of the weed community (581.2 pls m-2 - without straw and 8.3 pls m-2 - with straw). It is concluded that the U. decumbens straw as mulch is an effective tool in integrated management of weeds in cauliflower crops, without interfering with crop yield, and its potential to control weed community density is approximately 98.6%, at 22 days after transplanting. The adaptability of the cauliflower cultivar to the region has a high impact on weed management.
DOI: 10.6084/m9.figshare.10258355.v1
title: Bioaccumulation, Growth and Photosynthetic Response of a New Found in Bulgaria Invasive Species Lemna minuta and L. valdiviana to Heavy Metal Pollution

description: ABSTRACT: Heavy metals can meet in the surrounding environment as natural ingredients or from agricultural, industrial and chemical industries. The study was conducted in order to trace the potential of the aquatic plant L. minuta and L. valdiviana for the bioaccumulation of Cu, Cd, and Pb from contaminated water at low levels of these elements. Each of the duckweed species was treated separately with CuSO4.5H2O, CdSO4, Pb SO4 (Valerus, Bulgaria) at 0.5 and 1 mg L-1 concentrations of for 96 hours. After conducting the experiments, relative growth rate (RGR), bioconcentration factor (BCF), tolerant index (Ti) and photosynthetic pigments of two Lemna species were studied. The solution with higher metal concentration more inhibits the growth of macrophytes. The BCF of the metals on the two species were in decreasing order: Cu &gt; Pb &gt; Cd. Our study showed that L. minuta and L. valdiviana at a concentration of 0.5 mg L-1 copper have better affected on the photosynthetic apparatus compared to the control. Better bioaccumulation ability was established in L. minuta compared to L. valdiviana.
DOI: 10.6084/m9.figshare.10258346
title: Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, Northeastern Brazil

description: Abstract Equine piroplasmosis, an economically important disease in horses, has so far not been reported in Pernambuco state, Brazil. This study aimed to evaluate the seroprevalence of anti-Babesia caballi and anti-Theileria equi antibodies based on the detection of these agents in equine blood and in ticks on horses in the municipality of Petrolina, Pernambuco, northeastern Brazil. Blood samples were drawn from 393 horses and sera were examined by ELISA. The presence of tick infestations was evaluated, and 101 ticks were subjected to DNA amplification for the detection of Babesia spp. by polymerase chain reaction (PCR). No parasites were detected in the blood smears. Anti-B. caballi and anti-T. equi antibodies were found in 27.2% (107/393) and 34.8% (137/393) horses, respectively. Infestation by Dermacentor nitens was detected in 4.3% (17/393) of the horses. There was no DNA amplification of the agents in ticks. The risk factors for the presence of anti-T. equi antibodies (P &lt; 0.05) were: purebred (P &lt; 0.001), animals older than 156 months (P = 0.014), and the presence of ticks (P = 0.001). No risk factors for B. caballi were identified. This study confirmed the circulation of agents of equine piroplasmosis in the municipality of Petrolina, state of Pernambuco, Brazil.
DOI: 10.6084/m9.figshare.10258349
title: Germination of Stigmaphyllon blanchetii Seeds in Different Temperatures and Luminosity

description: ABSTRACT: The objective of the present work was to study the influence of temperature and light on germination of seeds of Stigmaphyllon blanchetii, popularly known as rat tail. The first stage of the research evaluated the effect of three constant temperatures (20 oC, 25 oC and 30 oC) and an alternating temperature (20-30 oC). In the second stage, for simulation of different types of light, four conditions of luminosity (white, red, far red and absence of light) were used. The temperatures that provided the best germination and development for S. blanchetii are the constant (30 oC) and the alternating (20-30 oC) ones. The seeds germinated both in the presence and absence of light, but there was greater germination and seedling development in the absence of light; thus they were classified as preferential negative photoblastic seeds.
DOI: 10.6084/m9.figshare.10258340
title: Autochthonous canine visceral leishmaniasis cases occur in Paraná state since 2012: isolation and identification of Leishmania infantum

description: Abstract The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Iguaçu. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 buffy coat samples were obtained mostly from the dogs that were seroreagents for leishmaniasis. In the second sample collection stage (2013), the buffy coat samples were collected from 376 dogs located close to Paraguay, Paraná river, center and peripheral parts of the city. The DNA from the six isolates, four from the first sampling stage (4/49) and two from the second sampling stage (2/376), was subjected to polymerase chain reaction using the K26F/R primers. The isolate was confirmed as L. infantum by sequencing. As none of the dogs had ever left the city, the isolates were confirmed as autochthonous. Further, the study confirmed the emergence of canine visceral leishmaniasis in Paraná through the identification of L. infantum among dogs in Foz do Iguaçu city. Hence, collaborative control measures should be designed and implemented by the public agencies and research institutions of Brazil, Argentina, and Paraguay to control the spread of visceral leishmaniasis.
DOI: 10.6084/m9.figshare.10258343
title: A new species of Coccoloba P. Browne (Polygonaceae) from the Brazilian Amazon with exceptionally large leaves

description: ABSTRACT We describe and illustrate a new species of Coccoloba (Polygonaceae), named Coccoloba gigantifolia, from the Brazilian Amazon. It resembles Coccoloba mollis Casar, but differs from the latter species by its much larger leaves in the fertile branches. The species has only been recorded in the Madeira River basin, in the states of Amazonas and Rondônia, in the central and southwestern Brazilian Amazon. The description was based on herbarium material, cultivated plants, and individual trees in their natural habitat. We provide illustrations, photographs, and an identification key with morphological characteristics that distinguish the new taxon from the other two related taxa of the Coccoloba sect. Paniculatae, as well as comments on the geographic distribution and conservation status of the species.
DOI: 10.6084/m9.figshare.10258346.v1
title: Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, Northeastern Brazil

description: Abstract Equine piroplasmosis, an economically important disease in horses, has so far not been reported in Pernambuco state, Brazil. This study aimed to evaluate the seroprevalence of anti-Babesia caballi and anti-Theileria equi antibodies based on the detection of these agents in equine blood and in ticks on horses in the municipality of Petrolina, Pernambuco, northeastern Brazil. Blood samples were drawn from 393 horses and sera were examined by ELISA. The presence of tick infestations was evaluated, and 101 ticks were subjected to DNA amplification for the detection of Babesia spp. by polymerase chain reaction (PCR). No parasites were detected in the blood smears. Anti-B. caballi and anti-T. equi antibodies were found in 27.2% (107/393) and 34.8% (137/393) horses, respectively. Infestation by Dermacentor nitens was detected in 4.3% (17/393) of the horses. There was no DNA amplification of the agents in ticks. The risk factors for the presence of anti-T. equi antibodies (P &lt; 0.05) were: purebred (P &lt; 0.001), animals older than 156 months (P = 0.014), and the presence of ticks (P = 0.001). No risk factors for B. caballi were identified. This study confirmed the circulation of agents of equine piroplasmosis in the municipality of Petrolina, state of Pernambuco, Brazil.
DOI: 10.6084/m9.figshare.10258349.v1
title: Germination of Stigmaphyllon blanchetii Seeds in Different Temperatures and Luminosity

description: ABSTRACT: The objective of the present work was to study the influence of temperature and light on germination of seeds of Stigmaphyllon blanchetii, popularly known as rat tail. The first stage of the research evaluated the effect of three constant temperatures (20 oC, 25 oC and 30 oC) and an alternating temperature (20-30 oC). In the second stage, for simulation of different types of light, four conditions of luminosity (white, red, far red and absence of light) were used. The temperatures that provided the best germination and development for S. blanchetii are the constant (30 oC) and the alternating (20-30 oC) ones. The seeds germinated both in the presence and absence of light, but there was greater germination and seedling development in the absence of light; thus they were classified as preferential negative photoblastic seeds.
DOI: 10.6084/m9.figshare.10258352.v1
title: Cauliflower Cultivars Associated with Urochloa decumbens Straw Mulch in Integrated Weed Management

description: ABSTRACT: In vegetable farming, the reduction in the use of herbicides has been an important factor in the definition of the production system. The objective of this research was to evaluate crop yield of cauliflower cultivars grown on Urochloa decumbens straw mulch, as well as the effect of the association between straw and cultivars on weed management. The treatments were arranged in a 2 x 4 factorial scheme: two managements (with or without U. decumbens straw mulch) and four cauliflower cultivars (Barcelona, Bromus, Júlia and Lisblanc). The experiment used a randomized block design with four replicates. There was no interference of straw mulch on crop yield of cauliflower cultivars. Hybrids Barcelona and Júlia presented yields of 22.0 and 23.9 t ha-1, respectively, and they did not differ from each other. The Bromus and Lisblanc materials did not adapt to the edaphoclimatic conditions of the region, which resulted in reduced cultural control and high weed emergence after weeding. U. decumbens straw had high control of the weed community (581.2 pls m-2 - without straw and 8.3 pls m-2 - with straw). It is concluded that the U. decumbens straw as mulch is an effective tool in integrated management of weeds in cauliflower crops, without interfering with crop yield, and its potential to control weed community density is approximately 98.6%, at 22 days after transplanting. The adaptability of the cauliflower cultivar to the region has a high impact on weed management.
DOI: 10.6084/m9.figshare.10258337
title: Growth and Development of Sourgrass Plants from Vegetative Parts of Clumps

description: ABSTRACT: Sourgrass (Digitaria insularis) is a perennial plant with high infestation potential, has fast and aggressive development, and reproduces by seeds and rhizomes. D. insularis infestations have increased in agricultural areas without cover crops established between the main crop seasons. The control of this species has become one of the most expensive in these areas in Brazil. The present work evaluated the growth and development of D. insularis plants from different vegetative parts of clumps. Two greenhouse experiments were conducted in a completely randomized design, with four replications. A 5 × 4 factorial arrangement was used, with factor A corresponding to the different propagation methods, and factor B corresponding to the biotypes used (experiment 1) and the different planting depths evaluated (experiment 2). The parameters evaluated in all experiments were: rate of tiller emergence, number of tillers, flowering time, number of inflorescences, and shoot dry weight. Fragments of different parts of the sourgrass clumps, planted at up to 7 cm depth, can generate a new plant. The management of perennial plants of sourgrass by using plows, harrows, or cultivators favors the dispersal of this species.
DOI: 10.6084/m9.figshare.10258340.v1
title: Autochthonous canine visceral leishmaniasis cases occur in Paraná state since 2012: isolation and identification of Leishmania infantum

description: Abstract The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Iguaçu. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 buffy coat samples were obtained mostly from the dogs that were seroreagents for leishmaniasis. In the second sample collection stage (2013), the buffy coat samples were collected from 376 dogs located close to Paraguay, Paraná river, center and peripheral parts of the city. The DNA from the six isolates, four from the first sampling stage (4/49) and two from the second sampling stage (2/376), was subjected to polymerase chain reaction using the K26F/R primers. The isolate was confirmed as L. infantum by sequencing. As none of the dogs had ever left the city, the isolates were confirmed as autochthonous. Further, the study confirmed the emergence of canine visceral leishmaniasis in Paraná through the identification of L. infantum among dogs in Foz do Iguaçu city. Hence, collaborative control measures should be designed and implemented by the public agencies and research institutions of Brazil, Argentina, and Paraguay to control the spread of visceral leishmaniasis.
DOI: 10.6084/m9.figshare.10258328
title: Resistance of the stable fly, Stomoxys calcitrans (Diptera: Muscidae), to cypermethrin in outbreak areas in Midwestern Brazil

description: Abstract Population explosions of the stable fly (Stomoxys calcitrans) have become a serious concern for livestock producers near sugarcane mills in some regions of Brazil due to the insect’s massive reproduction on sugarcane byproducts and waste. Despite the limited efficiency of insecticides for controlling stable fly outbreaks, producers still rely on chemical control to mitigate the alarming infestations in affected areas. This study evaluated the susceptibility of S. calcitrans populations to cypermethrin in the state of Mato Grosso do Sul, Brazil. Stable flies were tested from three field populations and two colonies, established from flies previously collected at sugarcane mills. Wild flies were collected with Nzi traps in areas of sugarcane plantations. Both wild and colonized flies were exposed to eleven concentrations of cypermethrin in impregnated filter paper bioassays. All the populations proved to be resistant to cypermethrin, with resistance factors among field populations ranging from 6.8 to 38.6. The intensive use of insecticides has led to the development of pyrethroid resistance in stable fly populations in the proximities of sugarcane mills in the state of Mato Grosso do Sul.
DOI: 10.6084/m9.figshare.10258334
title: Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil

description: Abstract The aim of this study was to investigate the occurrence of Leishmania spp. antibodies, and its association with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), in domestic cats from an area endemic for canine and human leishmaniasis in Rio Grande do Norte State, Brazil. Ninety-one cats were subjected to a complete clinical exam, and blood samples were collected. An epidemiological questionnaire was used to investigate the risk factors. IgG anti-Leishmania spp. antibodies were detected by immunofluorescence antibody test (IFAT), with a cut-off value of 1:40. Polymerase chain reaction (PCR) was performed to detect genetic material of Leishmania spp. in the blood samples. The presence of antibodies against FIV and antigens of FeLV was evaluated using an immunochromatographic test. Seropositivity for Leishmania spp., FIV, and FeLV was observed in 14/91 (15.38%), 26/91 (28.57%), and 3/91 (3.29%) cats, respectively. All samples gave negative results on PCR analysis. Based on these data, no significant statistical association was observed between seropositivity for Leishmania spp., and sex, age, presence of clinical signs, evaluated risk factors, and positivity for retroviruses. These findings demonstrated for the first time that cats from Mossoró, Rio Grande do Norte, are being exposed to this zoonosis and might be part of the epidemiological chain of transmission of visceral leishmaniasis.
DOI: 10.6084/m9.figshare.10258337.v1
title: Growth and Development of Sourgrass Plants from Vegetative Parts of Clumps

description: ABSTRACT: Sourgrass (Digitaria insularis) is a perennial plant with high infestation potential, has fast and aggressive development, and reproduces by seeds and rhizomes. D. insularis infestations have increased in agricultural areas without cover crops established between the main crop seasons. The control of this species has become one of the most expensive in these areas in Brazil. The present work evaluated the growth and development of D. insularis plants from different vegetative parts of clumps. Two greenhouse experiments were conducted in a completely randomized design, with four replications. A 5 × 4 factorial arrangement was used, with factor A corresponding to the different propagation methods, and factor B corresponding to the biotypes used (experiment 1) and the different planting depths evaluated (experiment 2). The parameters evaluated in all experiments were: rate of tiller emergence, number of tillers, flowering time, number of inflorescences, and shoot dry weight. Fragments of different parts of the sourgrass clumps, planted at up to 7 cm depth, can generate a new plant. The management of perennial plants of sourgrass by using plows, harrows, or cultivators favors the dispersal of this species.
DOI: 10.6084/m9.figshare.10258331
title: Biochemical profile of Mangalarga Marchador mares during the last third of pregnancy, foaling and lactation

description: ABSTRACT: Adaptive changes in metabolism may occur under specific physiological conditions, being necessary the knowledge of the biochemical constituents that can undergo variations during the sixty days before foaling, until the first sixty days of lactation. The present study aimed to identify and evaluate the biochemical profile of Mangalarga Marchador mares during the last third of pregnancy, foaling and lactation. For this, 48 mares were used, which formed a maintenance group (MG), composed of non-pregnant mares and an experimental group, called the transition group (TG), formed by pregnant mares. Blood samples were collected at the following times: MG was evaluated in a single time (beginning of all collections); TG was evaluated in seven times [T-60, T-30, T-15 (days before foaling), T0 (first six hours after foaling), T15, T30, T60 (days after foaling)]. All animals were submitted to biochemical analysis, in which the concentrations of total calcium, chloride, phosphorus, magnesium, sodium, potassium, uric acid, creatinine, and urea were measured. The two groups differed significantly (P&lt;0.05) in calcium, magnesium, sodium, potassium, creatinine, uric acid and urea concentration. Variation between times was also observed for these compounds. However, chloride and phosphorus did not differ between MG and TG. It was concluded that the last third of pregnancy, foaling and lactation exerts major influence on the biochemical constituents of blood through adjustments to maintain homeostasis in mares.