DOI: 10.22002/r21ex-jh471
title: Myxococcus xanthus LL061

description: mCherry-bacP.First loaded in Titan cartridge and imaged in cryoLM.Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 29100x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-1/rawdata/</p>LL061_UCLA_correlated_20130424_24.mrc,  Tilt Series (Pixel Size 1.0 nm), 1.1 GB  
        
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        LL061_UCLA_correlated_20130424_24_full.rec,  Reconstruction (Pixel Size 2.0 nm), 629.1 MB  
        
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        LL061_UCLA_correlated_20130424_24.rec,  Reconstruction (Pixel Size 2.0 nm), 419.4 MB  
        
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        LL061_UCLA_correlated_20130424_24_bin2_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 419.4 MB  
        
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        slicer000_DB.tif,  4.0 MB  
        
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        cryoLM_DB.tif,  4.3 MB  
        
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        slicer001_DB.tif,  3.1 MB  
        
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