cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6388280
title: Antibodies anti-trypanosomatides in domestic cats in Paraná: who is at highest risk of infection?

description: Abstract The aim of this study were to detect antibodies anti-Leishmania spp. and anti-Trypanosoma cruzi in two different populations of domestic cats (Felis catus domesticus) from North Paraná referred for surgical castration and to determine which characteristics of the animals studied may be associated with seropositivity. Serum samples from 679 cats were analyzed using enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT) in series. Associations between age, sex, race, year of care and animal group were verified using the simple logistic regression. Percentage of 8.5% (58/679) of cats were positive for Leishmania spp. and 7.6% (51/673) for T. cruzi by the tests ELISA and IFAT. Animals collected by non-governmental animal protection organizations presented more seropositivity for Leishmania spp. (p&lt;0.0001). Results shown that Leishmania spp. and T. cruzi are present in domestic cats in the northern part of the state of Paraná, as well as, owners of non-governmental animal protection organizations may be more exposed to leishmaniasis when compared to other animal owners evaluated in the present study.
DOI: 10.6084/m9.figshare.6388280.v1
title: Antibodies anti-trypanosomatides in domestic cats in Paraná: who is at highest risk of infection?

description: Abstract The aim of this study were to detect antibodies anti-Leishmania spp. and anti-Trypanosoma cruzi in two different populations of domestic cats (Felis catus domesticus) from North Paraná referred for surgical castration and to determine which characteristics of the animals studied may be associated with seropositivity. Serum samples from 679 cats were analyzed using enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT) in series. Associations between age, sex, race, year of care and animal group were verified using the simple logistic regression. Percentage of 8.5% (58/679) of cats were positive for Leishmania spp. and 7.6% (51/673) for T. cruzi by the tests ELISA and IFAT. Animals collected by non-governmental animal protection organizations presented more seropositivity for Leishmania spp. (p&lt;0.0001). Results shown that Leishmania spp. and T. cruzi are present in domestic cats in the northern part of the state of Paraná, as well as, owners of non-governmental animal protection organizations may be more exposed to leishmaniasis when compared to other animal owners evaluated in the present study.
DOI: 10.6084/m9.figshare.6388277
title: Follow up of a robust meta-signature to identify Zika virus infection in Aedes aegypti: another brick in the wall

description: The mosquito Aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. In a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, West Nile and yellow fever viruses. Of these 110 genes, four genes allowed a highly accurate classification of infected status. More recently, a new study of Ae. aegypti infected with Zika virus (ZIKV) was published, providing new data to investigate whether this “infection” gene set is also altered during a ZIKV infection. Our hypothesis is that the infection-associated signature may also serve as a proxy to classify the ZIKV infection in the vector. Raw data associated with the NCBI/BioProject were downloaded and re-analysed. A total of 18 paired-end replicates corresponding to three ZIKV-infected samples and three controls were included in this study. The nMDS technique with a logistic regression was used to obtain the probabilities of belonging to a given class. Thus, to compare both gene sets, we used the area under the curve and performed a comparison using the bootstrap method. Our meta-signature was able to separate the infected mosquitoes from the controls with good predictive power to classify the Zika-infected mosquitoes.
DOI: 10.6084/m9.figshare.6388277.v1
title: Follow up of a robust meta-signature to identify Zika virus infection in Aedes aegypti: another brick in the wall

description: The mosquito Aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. In a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, West Nile and yellow fever viruses. Of these 110 genes, four genes allowed a highly accurate classification of infected status. More recently, a new study of Ae. aegypti infected with Zika virus (ZIKV) was published, providing new data to investigate whether this “infection” gene set is also altered during a ZIKV infection. Our hypothesis is that the infection-associated signature may also serve as a proxy to classify the ZIKV infection in the vector. Raw data associated with the NCBI/BioProject were downloaded and re-analysed. A total of 18 paired-end replicates corresponding to three ZIKV-infected samples and three controls were included in this study. The nMDS technique with a logistic regression was used to obtain the probabilities of belonging to a given class. Thus, to compare both gene sets, we used the area under the curve and performed a comparison using the bootstrap method. Our meta-signature was able to separate the infected mosquitoes from the controls with good predictive power to classify the Zika-infected mosquitoes.
DOI: 10.6084/m9.figshare.6388274
title: Parasitic profile of traction equids in the semi-arid climate of Paraíba State, Northeastern, Brazil

description: Abstract The objective of this study was to determine the parasitological profiles of traction equids from the semi-arid climate of Paraíba (PB) State. We analyzed 96 equids that performed traction work, with no defined breed, males and females, and all older than 24 months. Among the analyzed animals, 51% were donkeys, 41.7% mules, and 7.3% horses. Fecal material and blood were collected for parasitological examination and to determine the packed cell volume (PCV), respectively. The samples were sent to the Veterinary Parasitology Laboratory, Veterinary Hospital - IFPB, Sousa-PB campus, and epidemiological questionnaires were provided to the owners. It was observed that 83.3% of equids tested positive for gastrointestinal parasites. Strongylidae was the most prevalent parasite (83.3%), followed by Giardia sp. (5.3%), and Eimeria sp. (2.1%). The mean eggs per gram (EPG) value of the fecal samples was 1143. Fecal analysis revealed that 78.9% of the larvae were from “small strongyles”. The presence of ectoparasites was not observed in the studied animals. The answers to our questionnaire revealed that 69.8% of the animals had never been dewormed and that the remaining 31.2% had only been dewormed once. We concluded that the prevalence of gastrointestinal parasites among traction equids in the municipality of Sousa-PB is high.
DOI: 10.6084/m9.figshare.6388274.v1
title: Parasitic profile of traction equids in the semi-arid climate of Paraíba State, Northeastern, Brazil

description: Abstract The objective of this study was to determine the parasitological profiles of traction equids from the semi-arid climate of Paraíba (PB) State. We analyzed 96 equids that performed traction work, with no defined breed, males and females, and all older than 24 months. Among the analyzed animals, 51% were donkeys, 41.7% mules, and 7.3% horses. Fecal material and blood were collected for parasitological examination and to determine the packed cell volume (PCV), respectively. The samples were sent to the Veterinary Parasitology Laboratory, Veterinary Hospital - IFPB, Sousa-PB campus, and epidemiological questionnaires were provided to the owners. It was observed that 83.3% of equids tested positive for gastrointestinal parasites. Strongylidae was the most prevalent parasite (83.3%), followed by Giardia sp. (5.3%), and Eimeria sp. (2.1%). The mean eggs per gram (EPG) value of the fecal samples was 1143. Fecal analysis revealed that 78.9% of the larvae were from “small strongyles”. The presence of ectoparasites was not observed in the studied animals. The answers to our questionnaire revealed that 69.8% of the animals had never been dewormed and that the remaining 31.2% had only been dewormed once. We concluded that the prevalence of gastrointestinal parasites among traction equids in the municipality of Sousa-PB is high.
DOI: 10.6084/m9.figshare.6388271
title: First record of Ornithodoros faccinii (Acari: Argasidae) on toads of genus Rhinella (Anura: Bufonidae) in Brazil

description: Abstract Although a group of soft ticks (Argasidae) associated with amphibians was recently discovered in Brazilian rainforests, parasitism by these ticks on cold-blooded animals remains less common than on mammal and bird species. In this study, we identified ticks that were collected from toads that had been caught in December 2016 and January 2017, at Itinguçú waterfall (22°54’05” S; 43°53’30” W) in the municipality of Itaguaí, state of Rio de Janeiro. Tick specimens were identified using a morphological and molecular approach. In total, twelve larvae of Ornithodoros ticks were collected from three individuals of Rhinella ornata and were identified as Ornithodoros faccinii. Our results include a longer 16S rRNA mitochondrial sequence for O. faccinii that supports its phylogenetic relatedness to Ornithodoros saraivai, and we report this tick species parasitizing Rhinella toads for the first time in Brazil.
DOI: 10.6084/m9.figshare.6388271.v1
title: First record of Ornithodoros faccinii (Acari: Argasidae) on toads of genus Rhinella (Anura: Bufonidae) in Brazil

description: Abstract Although a group of soft ticks (Argasidae) associated with amphibians was recently discovered in Brazilian rainforests, parasitism by these ticks on cold-blooded animals remains less common than on mammal and bird species. In this study, we identified ticks that were collected from toads that had been caught in December 2016 and January 2017, at Itinguçú waterfall (22°54’05” S; 43°53’30” W) in the municipality of Itaguaí, state of Rio de Janeiro. Tick specimens were identified using a morphological and molecular approach. In total, twelve larvae of Ornithodoros ticks were collected from three individuals of Rhinella ornata and were identified as Ornithodoros faccinii. Our results include a longer 16S rRNA mitochondrial sequence for O. faccinii that supports its phylogenetic relatedness to Ornithodoros saraivai, and we report this tick species parasitizing Rhinella toads for the first time in Brazil.
DOI: 10.6084/m9.figshare.6388268
title: Evaluation of the fetal abdomen by magnetic resonance imaging. Part 1: malformations of the abdominal cavity

description: Abstract Although ultrasound continues to be the mainstay modality for the evaluation of fetal disorders, fetal magnetic resonance imaging (MRI) has often been used as a valuable adjunct in recent years. The exponential growth of the use of fetal MRI has been facilitated by technological advancements such as ultrafast T2-weighted sequences and diffusion-weighted imaging. Fetal MRI can achieve results that are comparable to or better than those of ultrasound, particularly in cases of maternal obesity, severe oligohydramnios, or abnormal fetal position. Because of its superior soft tissue contrast, wide field of view, and multiplanar imaging, fetal MRI is able to evaluate the large fetal organs, such as the lungs, liver, bowel, and kidneys. In addition, fetal MRI allows large or complex malformations to be examined, facilitating the understanding of the malformation within the context of the body as a whole. Initial fetal MRI studies were focused on the central nervous system. With advances in software and hardware, fetal MRI gained importance in the evaluation of the fetal abdomen. The purpose of this article is to review the recent literature and developments in MRI evaluation of the fetal abdomen, with an emphasis on imaging aspects, protocols, and common clinical indications.
DOI: 10.6084/m9.figshare.6388265
title: Frequency of anti-Toxocara antibodies in broiler chickens in southern Brazil

description: Abstract The aim of this study was to evaluate the presence of anti-Toxocara antibodies in naturally infected broiler chickens (n = 189) from the state of Paraná, southern Brazil. The chickens were reared in a semi-intensive system by small family farmers (n = 7). An enzyme-linked immunosorbent assay (ELISA) was performed to detect the presence of anti- Toxocara spp. IgY after serum adsorption with Ascaridia galli antigens. An overall seroprevalence of 67.7% (128/189; 95% CI = 61.1-74.4) was observed. The frequency of positive animals by farm ranged from 29.6% to 100%. The optical density and reactivity index values observed in ELISA test indicated the possible chronicity of infection of the evaluated chickens. Associations between the presence of antibodies and the area where the chickens were reared (p = 0.382) or the population density of dogs on the farm (p = 0.785) were not observed. This study shows a high prevalence of Toxocara spp. antibodies in broiler chickens reared in semi-intensive systems and provides evidence that chickens are a good indicator of environmental contamination by larva migrans agents. Further studies are necessary to assess the risk factors associated with poultry infection and the likelihood of toxocariasis transmission to humans via the ingestion of free-range chicken meat.
DOI: 10.6084/m9.figshare.6388265.v1
title: Frequency of anti-Toxocara antibodies in broiler chickens in southern Brazil

description: Abstract The aim of this study was to evaluate the presence of anti-Toxocara antibodies in naturally infected broiler chickens (n = 189) from the state of Paraná, southern Brazil. The chickens were reared in a semi-intensive system by small family farmers (n = 7). An enzyme-linked immunosorbent assay (ELISA) was performed to detect the presence of anti- Toxocara spp. IgY after serum adsorption with Ascaridia galli antigens. An overall seroprevalence of 67.7% (128/189; 95% CI = 61.1-74.4) was observed. The frequency of positive animals by farm ranged from 29.6% to 100%. The optical density and reactivity index values observed in ELISA test indicated the possible chronicity of infection of the evaluated chickens. Associations between the presence of antibodies and the area where the chickens were reared (p = 0.382) or the population density of dogs on the farm (p = 0.785) were not observed. This study shows a high prevalence of Toxocara spp. antibodies in broiler chickens reared in semi-intensive systems and provides evidence that chickens are a good indicator of environmental contamination by larva migrans agents. Further studies are necessary to assess the risk factors associated with poultry infection and the likelihood of toxocariasis transmission to humans via the ingestion of free-range chicken meat.
DOI: 10.5281/zenodo.4007762
title: Storage. Speicherung von Forschungsdaten. SUH.

description: Leitfragen zur Ermittlung des Bedarfs:<br> 1. Wie viel Speicher benötigen Sie im Durchschnitt?<br> 2. Welche Kriterien müsste eine Infrastruktur erfüllen, um Ihren<br> Anforderungen gerecht zu werden? Die Fragen an das Forschungsprojekt beziehen sich auf Ihre<br> Forschungsdaten:<br> ● Welche Datentypen und welche Datenformate fallen in Ihrem Projekt<br> an?<br> ● Bitte schätzen Sie Umfang/Volumen der Forschungsdaten ein<br> (ungefähre Größenordnung, d.h. weniger als 100 GB, 1 TB, 10 TB,<br> 100 TB)?<br> ● Können Sie Aussagen zur Datenqualität machen? (z.B. Text: Wie<br> viele Seiten? PDF, Word? Printqualität, Webqualität u.ä.;<br> Audiointerview: Wieviel Stunden? Stereo, Mono? Ist eine<br> Sendequalität erforderlich?<br> ● Sind die Daten besonders schutzwürdig oder öffentlich (Frage nach<br> der Sensibilität)?<br> ● Wo speichern Sie die Forschungsdaten, die im Rahmen Ihrer<br> Projekte anfallen und zu welchem Zweck sollen die Daten aufbewahrt<br> werden? (mit Angabe der Aufbewahrungsdauer, wenn möglich)<br> ● Werden Sie Ihre Daten lokal oder in einem fachspezifischen,<br> externen Repositorium speichern (mit Begründung, wenn möglich)?<br> ● Mit welchen Metadaten bzw. Metadatenstandards arbeiten Sie im<br> Projekt (sofern dies schon der Fall sein sollte)?<br> Metadaten sind strukturierte Informationen, die die vorliegenden<br> Daten näher beschreiben. Während formatspezifische Metadaten,<br> auch technische Metadaten genannt, Auskunft über Dateityp, -größe<br> und Erstelldatum geben, enthalten deskriptive Metadaten<br> Informationen über den Inhalt der Daten, die die Auffindbarkeit,<br> Nachnutzung und Zitierbarkeit der Daten erleichtern.<br> UNEKE zielte insbesondere in der wisseschaftlichen Begleitstudie auf die Frage, was<br> Forschende abhält bzw. ermutigt zukünftig Daten zu publizeren und zu teilen.
DOI: 10.6084/m9.figshare.6388262
title: Prevalence of bovine fascioliasis, areas at risk and ensuing losses in the state of Goiás, Brazil

description: Abstract The present study had to determine the prevalence and spatial distribution of areas at risk of bovine fascioliasis in the state of Goiás, central-western Brazil between 2007 and 2014; to evaluate the associations of some epidemiological variables with occurrences of Fasciola hepatica in animals; and to estimate the economic losses that this parasite on the cattle industry. It could be concluded that of 23,255,979 animals slaughtered, the average prevalence of F. hepatica in cattle in Goiás during the period evaluated was 0.0026% (95% CI: 0.0024-0.0028). In the State of Goias, in about then years (since when this parasite was found for the first time by other researchers in 2007), F. hepatica was diagnosed in 168 new municipalities. Using the regression analysis, the effective bovine herd size was a significant risk factor (OR= 1.21; 95% CI 1.1022-1.4510; p ≤ 0.05) for cattle to be infected with fascioliasis in the state of Goiás. The cattle-rearing industry lost approximately R$ 15,072.75 (US$ 4,785) due to condemn of livers with Fasciola in the state of Goiás. New studies need to be conducted in these regions, with the aim to identify the likelihood of presence of intermediate hosts, which might serve as a source of F. hepatica infection for definitive hosts.
DOI: 10.6084/m9.figshare.6388262.v1
title: Prevalence of bovine fascioliasis, areas at risk and ensuing losses in the state of Goiás, Brazil

description: Abstract The present study had to determine the prevalence and spatial distribution of areas at risk of bovine fascioliasis in the state of Goiás, central-western Brazil between 2007 and 2014; to evaluate the associations of some epidemiological variables with occurrences of Fasciola hepatica in animals; and to estimate the economic losses that this parasite on the cattle industry. It could be concluded that of 23,255,979 animals slaughtered, the average prevalence of F. hepatica in cattle in Goiás during the period evaluated was 0.0026% (95% CI: 0.0024-0.0028). In the State of Goias, in about then years (since when this parasite was found for the first time by other researchers in 2007), F. hepatica was diagnosed in 168 new municipalities. Using the regression analysis, the effective bovine herd size was a significant risk factor (OR= 1.21; 95% CI 1.1022-1.4510; p ≤ 0.05) for cattle to be infected with fascioliasis in the state of Goiás. The cattle-rearing industry lost approximately R$ 15,072.75 (US$ 4,785) due to condemn of livers with Fasciola in the state of Goiás. New studies need to be conducted in these regions, with the aim to identify the likelihood of presence of intermediate hosts, which might serve as a source of F. hepatica infection for definitive hosts.
DOI: 10.6084/m9.figshare.6388256
title: Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil

description: Abstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.
DOI: 10.6084/m9.figshare.6388256.v1
title: Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil

description: Abstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.
DOI: 10.6084/m9.figshare.6388253
title: Ectoparasites from some Myocastor coypus (Molina, 1782) populations (Coypus or Nutria) in Argentina

description: Abstract The occurrence of ectoparasites in wild nutria is poorly understood. Fifty-five livetrapped wild nutria (Myocastor coypus) from its indigenous region were examined for ectoparasites after capture from December 2013 to December 2014. The captures came from the Buenos Aires Province, by far the area of the country most densely populated by nutria, characterized as a temperate grassland, which are prime areas for sustained agriculture. Only one species of chewing lice (Pitrufquenia coypus, Marelli, 1932), one flea (Nosopsyllus fasciatus, Bosc, 1800) and one tick (Rhipicephalus sanguineus, Latreille, 1806) were collected. Fourteen percent of the animals were infested and P.coypus, an obligate parasite of the nutria, which was the most prevalent ectoparasite. N. fasciatus and R. sanguineus occurrence remains controversial as they may or may not be some accidental host species. To our knowledge, this is the first comprehensive and systematic survey of ectoparasites in wild nutria from the southern hemisphere, the indigenous region of this species.