cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/dwr4y-25n35
title: COS7 VPS4DN+CHMP4A

description: Cos7 cells co-transfected with VPS4DN and CHMP4A. Sample #C104
description: <strong>Tilt Series Date:</strong> 2012-02-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN and CHMP4A constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-23/3dimage_68725/</p>12feb04_ATBT1_0025_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-23/3dimage_68725/12feb04_ATBT1_0025_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0025.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-23/rawdata/12feb04_ATBT1_0025.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0025.tiff,  file associated to 3D Image #68725, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-23/file_15967/12feb04_ATBT1_0025.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jnb38-s9d21
title: Cos7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A+VPS4DN constructs. C116-C117
description: <strong>Tilt Series Date:</strong> 2012-02-27</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4DN constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-27-3/3dimage_68771/</p>12feb27_grid4_atlas1bBT1_0003_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-27-3/3dimage_68771/12feb27_grid4_atlas1bBT1_0003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb27_grid4_atlas1bBT1_0003.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-27-3/rawdata/12feb27_grid4_atlas1bBT1_0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb27_grid4_atlas1bBT1_0003_full.tiff,  file associated to 3D Image #68771, 1.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-27-3/file_15979/12feb27_grid4_atlas1bBT1_0003_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0an3y-x7377
title: Myxococcus xanthus LL061

description: mCherry-bacP.First loaded in Titan cartridge and imaged in cryoLM.Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 29100x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-16/rawdata/</p>LL061_UCLA_correlated_20130424_3.mrc,  Tilt Series (Pixel Size 1.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-16/rawdata/LL061_UCLA_correlated_20130424_3.mrc"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_3_full.rec,  Reconstruction (Pixel Size 2.0 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-16/3dimage_26291/LL061_UCLA_correlated_20130424_3_full.rec"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_3_bin2_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 314.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-16/3dimage_26789/LL061_UCLA_correlated_20130424_3_bin2_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.4s8gpj
title: Occurrence Download

description: A dataset containing 519 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((104.50007 -7.2253,114.85533 -8.88929,114.3465 -8.32546,114.96534 -6.96401,106.02654 -5.68507,104.50007 -7.2253))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Dipterocarpaceae"
 ]
}

 The dataset includes 519 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044050-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/88s81-20r10
title: COS7 VPS4DN+CHMP4A

description: Cos7 cells co-transfected with VPS4DN and CHMP4A. Sample #C104
description: <strong>Tilt Series Date:</strong> 2012-02-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN and CHMP4A constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-1/3dimage_68680/</p>12feb04_ATBT1_0001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-1/3dimage_68680/12feb04_ATBT1_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0001.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-1/rawdata/12feb04_ATBT1_0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0001_full.tiff,  file associated to 3D Image #68680, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-1/file_15946/12feb04_ATBT1_0001_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zzc5w-yat50
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-21</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-11/rawdata/</p>tomography2n.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-11/rawdata/tomography2n.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography2n.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-11/3dimage_47340/tomography2n.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography2n_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-11/3dimage_48769/tomography2n_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7xvf1-px368
title: COS7 VPS4DN+CHMP4A

description: Cos7 cells co-transfected with VPS4DN and CHMP4A. Sample #C104
description: <strong>Tilt Series Date:</strong> 2012-02-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN and CHMP4A constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-19/3dimage_68717/</p>12feb04_ATBT1_0021_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-19/3dimage_68717/12feb04_ATBT1_0021_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0021.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-19/rawdata/12feb04_ATBT1_0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0021_full.tiff,  file associated to 3D Image #68717, 1.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-19/file_15963/12feb04_ATBT1_0021_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ddb6g-tfb03
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-2/3dimage_68851/</p>12jan06_c105_1_4ADNCos7_AT1_man3_full.rec,  Reconstruction (Pixel Size 2.0 nm), 697.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-2/3dimage_68851/12jan06_c105_1_4ADNCos7_AT1_man3_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT1_man3.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-2/rawdata/12jan06_c105_1_4ADNCos7_AT1_man3.st"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT1_man3_full.tiff,  file associated to 3D Image #68851, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-2/file_16014/12jan06_c105_1_4ADNCos7_AT1_man3_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1mtek-r0e98
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-27/3dimage_69051/</p>12jan20_C110_2_pNL_INFalpha_AT1BT1_0006_full.rec,  Reconstruction (Pixel Size 2.0 nm), 650.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-27/3dimage_69051/12jan20_C110_2_pNL_INFalpha_AT1BT1_0006_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_2_pNL_INFalpha_AT1BT1_0006.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-27/rawdata/12jan20_C110_2_pNL_INFalpha_AT1BT1_0006.st"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_2_pNL_INFalpha_AT1BT1_0006_full.tiff,  file associated to 3D Image #69051, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-27/file_16103/12jan20_C110_2_pNL_INFalpha_AT1BT1_0006_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rc9pf-1xn71
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-2/rawdata/</p>tomography2middle.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-2/rawdata/tomography2middle.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography2middle.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-2/3dimage_47358/tomography2middle.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography2middle_full.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-2/3dimage_47856/tomography2middle_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8c2s43
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Zosterops hypolais",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044051-250811113504898/datasets/export for details.
DOI: 10.22002/fajd8-paf93
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-2/3dimage_68941/</p>12jan14_C107_1_pNL_DN_AT0man2_full.rec,  Reconstruction (Pixel Size 2.0 nm), 495.5 MB  
        
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        12jan14_C107_1_pNL_DN_AT0man2.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        12jan14_C107_1_pNL_DN_AT0man2_full.tiff,  file associated to 3D Image #68941, 1.3 MB  
        
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