DOI: 10.5061/dryad.80gb5mkws
title: Inverse priority effects: The order and timing of removal of invasive species influence community reassembly

description: 1. An ongoing restoration challenge is to recover native communities after
 the removal of invasive species. Because priority effects (i.e., the order
 and timing of species arrival) can strongly determine the trajectory of
 community assembly, their intentional manipulation is gaining attention to
 manage invasive plants and achieve restoration goals. Yet, ecologists and
 conservationists rarely consider how the order and timing of species
 removal inverse priority effect may impact future plant communities. 2.
 Here, we evaluated the dependence of community reassembly on inverse
 priority effects by experimentally removing the target invasives
 Sweetbriar rose (Rosa rubiginosa) and Scotch broom (Cytisus scoparius) in
 field and mesocosm communities. We manipulated removal order
 (rose-before-broom vs. broom-before-rose) and timing (simultaneously early
 vs. simultaneously late in the season). We performed a Hierarchical
 Modeling of Species Community to assess differences in community structure
 in response to order and timing of removal, and to evaluate whether
 species origin and leaf and seed traits were associated with species
 responses. 3. We found that the order of removal was as important as
 timing driving community reassembly. Simultaneous removal favoured
 nonnatives, more so when performed early. Sequential removals led to
 contrasting communities. Rose-before-broom removal also favoured nonnative
 grasses at expense of native species, whereas the inverse order produced
 small changes in communities. In general, species with high specific leaf
 area were boosted, regardless of their seed size. 4. Synthesis and
 applications. Inverse priority effects are neglected mechanisms that can
 drive variability in the reassembly of plant communities and can
 potentially upgrade invasive species management. These historical
 contingencies suggest the existence of an optimal order of removal that
 facilitates the recovery of the native community. We found that
 simultaneous removal promoted secondary invasions to a greater extent than
 sequential removals. Furthermore, removal order affected post-removal
 community structure. In our system, we suggest removing the rose before
 the broom to hinder nonnatives and pave the way for restoration of native
 communities. Our results show that manipulation of the order and timing of
 removal can help to achieve restoration goals.
description: Provenance for this README
 \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* File name:
 README\_Dataset-InversePriorityEffects.txt \* Authors: Agostina Torres,
 Teresa Morán-Lopez, Mariano A. Rodriguez-Cabal, and Martin A. Nuñez \*
 Date created: 2023-10-16 \* Date modified: 23-10-17 Dataset Attribution
 and Usage \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \*
 Dataset Title: Data from: Inverse priority effects: The order and timing
 of removal of invasive species influence community reassembly \*
 Persistent Identifier: 10.5061/dryad.80gb5mkws \* Dataset creators:
 Agostina Torres, Teresa Morán-Lopez, Mariano A. Rodriguez-Cabal, and
 Martin A. Nuñez \* Suggested Citations: \* Dataset citation: &gt;
 Torres, A., Morán-Lopez, T., Rodríguez-Cabal, M.A., and Nuñez, M.A. 2023.
 Data from: Inverse priority effects: The order and timing of removal of
 invasive species influence community reassembly. Dryad Digital Repository,
 https://doi.org/10.5061/dryad.80gb5mkws \* Corresponding publication:
 &gt; Torres, A., Morán-Lopez, T., Rodríguez-Cabal, M.A., and Nuñez,
 M.A. 2023. Inverse priority effects: The order and timing of removal of
 invasive species influence community reassembly. Journal of Applied
 Ecology. Accepted. Contact Information ------------------- \* Name:
 Agostina Torres \* Affiliations: Instituto de Investigaciones en
 Biodiversidad y Medioambiente, CONICET, Universidad Nacional del Comahue,
 Bariloche, Argentina. \* ORCID ID: https://orcid.org/0000-0001-9682-7296
 \* Email: torresa@comahue-conicet.gob.ar \* Address: e-mail preferred \-
 \- \- Dataset Metadata =========================== Dates and Locations
 ------------------- \* Dates of data collection: Field experiment
 conducted from February 2018 to January 2020. Mesocosm (greenhouse)
 experiment conducted from January 2018 to January 2020. \* Geographic
 locations of data collection: Fieldwork conducted in Isla Victoria (Parque
 Nacional Nahuel Hiapi). Mesocosm experiment conducted in the greenhouse of
 the Universidad Nacional del Comahue in Bariloche (Argentina). See
 publication for more details. \- \- \- Methodological Information
 ========================== \* Methods of data collection/generation: We
 estimated species cover (field experiment) or biomass (mesocosm experimet)
 of nontarget (nonremoved) species one/two years after removal of Cytisus
 scoparius and Rosa rubiginosa in different order and timing, both in field
 and mesocosm settings, respectively. See publication for more details on
 experiments. \* Data analysis: The response of nontarget species to
 different removal histories was evaluated through Hierarchical Models of
 Species Communities (HMSC). In field models, we also evaluated the effect
 of origin, functional traits (SLA and seed mass) and phylogenetic
 relationships on species responses to removals. In mesocosm models, we
 only evaluated the effect of origin on species responses. See publication
 for more details on models. \- \- \- Data and File Overview
 ====================== Summary Metrics --------------- \* Compressed file
 formats: .zip \* File formats: .csv \* Compressed file count: 2 \* Total
 .csv file count: 7 (4+3) Naming Conventions ------------------ \* File
 naming scheme: - First part: denotes the type of experiment,
 Field/Mesocosm. - Second part (for .csv):denotes the type of
 variables/information provided in each file. --&gt; Parts in naming
 schemes are separated by '\_'. Table of Contents
 ----------------- \* Field.zip - Field\_Treatments.csv - Field\_Cover.csv
 - Field\_Traits.csv - Field\_Phylogeny.csv \* Mesocosm.zip -
 Mesocosm\_Treatments.csv - Mesocosm\_Biomass.csv - Mesocosm\_Origin.csv
 Setup ----- \* Unpacking instructions: n/a \* Recommended software/tools:
 RStudio 2022.07.1; R version 4.1.1 \- \- \- File/Folder Details
 =================== Details for: Field\_Treatments.csv
 \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the treatment applied in each experimental plot in field experiment. \* Format(s): .csv \* Size(s): 1 KB \* Dimensions: 51 rows x 6 columns \* Variables: \* plot\_id: unique identification for each experimental plot \* columns 2-6: five unique identification code for removal treatments: 1rm = early simultaneous removal of Cytisus scoparius and Rosa rubiginosa 2rm = late simultaneous removal of Cytisus scoparius and Rosa rubiginosa 1r2m = removal of Cytisus scoparius three months before removal of Rosa rubiginosa 1m2r = removal of Rosa rubiginosa three months before removal of Cytisus scoparius control = no removal Details for: Field\_Cover.csv \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the cover of each nontarget species in each experimental plot in field experiment. \* Format(s): .csv \* Size(s): 6 KB \* Dimensions: 51 rows x 41 columns \* Variables: \* plot\_id: unique identification for each experimental plot \* Columns 2 to 41: the cover (%) of each nontarget species in the format 'Genus\_species' Details for: Field\_Traits.csv \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the traits of each nontarget species in field experiment. \* Format(s): .csv \* Size(s): 2 KB \* Dimensions: 42 rows x 4 columns \* Variables: \* Species: each of the 41 nontarget species in the format 'Genus\_species' \* Seed\_mass: nontarget species seed mass (mg). See publication for trait data imputation \* SLA: nontarget species specific leaf area (mm2/mg). See publication for trait data imputation \* Origin: nontarget species origin (native / nonnative). See publication for trait data imputation. Details for: Field\_Phylogeny.csv \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the phylogenetic relationships between species in field experiment. \* Format(s): .csv \* Size(s): 26 KB \* Dimensions: 41 rows x 41 columns \* A symmetric matrix of n-species x n-species containing the phylogenetic relationships. See publication for phylogeney imputation. Details for: Mesocosm\_Treatments.csv \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the treatment applied in each experimental pot in mesocosm experiment. \* Format(s): .csv \* Size(s): 1 KB \* Dimensions: 66 rows x 6 columns \* Variables: \* pot\_id: unique identification for each experimental pot \* Columns 2-6: five unique identification code for removal treatments. 1rm = early simultaneous removal of Cytisus scoparius and Rosa rubiginosa 2rm = late simultaneous removal of Cytisus scoparius and Rosa rubiginosa 1r2m = removal of Cytisus scoparius one month before removal of Rosa rubiginosa 1m2r = removal of Rosa rubiginosa one month before removal of Cytisus scoparius control = random removal of nontarget plants Details for: Mesocosm\_Biomass.csv \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the biomass of each nontarget species in each experimental pot in mesocosm. \* Format(s): .csv \* Size(s): 3 KB \* Dimensions: 66 rows x 7 columns \* Variables: \* pot\_id: unique identification for each experimental pot \* Columns 2 to 7: the biomass (g) of each nontarget species in the format 'Genus': - Cirsium = Cirsium vulgare - Cynoglossum = Cynoglossum creticum - Festuca = Festuca arundinacea - Acaena = Acaena splendens - Mutisia = Mutisia spinosa - Pappostipa = Pappostipa speciosa Details for: Mesocosm\_Origin.csv \-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\- \* Description: a comma-delimited file containing the origin of each nontarget species in mesocosm. \* Format(s): .csv \* Size(s): 1 KB \* Dimensions: 7 rows x 2 columns \* Variables: \* species: the name of each nontarget species in the format 'Genus': - Cirsium = Cirsium vulgare - Cynoglossum = Cynoglossum creticum - Festuca = Festuca arundinacea - Acaena = Acaena splendens - Mutisia = Mutisia spinosa - Pappostipa = Pappostipa speciosa \* origin: the origin of each species (native / nonnative) \- \- \- END OF README