cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/xnvpn-k7491
title: P. gingivalis wild type

description: Raw data files of P. gingivalis wild type
description: <strong>Tilt Series Date:</strong> 2020-11-06</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown and harvested from plates</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-346/rawdata/</p>PginWT9_345.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-346/rawdata/PginWT9_345.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT9_345_part38_4.rec,  Reconstruction (Pixel Size 1.332 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-346/3dimage_115650/PginWT9_345_part38_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wa41z-dsz23
title: Dual-labeled VSVG-HIV

description: Raw data files of Dual-labeled VSVG-HIV
description: <strong>Tilt Series Date:</strong> 2019-02-01</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> iMOD</p>
<strong>Collaborators and Roles:</strong> Thomas J. Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degrees celsius</p>
<strong>Sample Preparation:</strong> plunge-freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-01-16/rawdata/</p>V16.mrc,  Tilt Series (Pixel Size 0.351 nm), 3.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-01-16/rawdata/V16.mrc"
        > <i class="download icon"></i> Download </a></p>

        V16_part106_3.rec,  Reconstruction (Pixel Size 1.404 nm), 267.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-01-16/3dimage_101334/V16_part106_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.13777030
title: Integrated Healthcare Services' Health Statistical Data on Regional Global Budget Payment System in Zhejiang Province, China"
DOI: 10.5281/zenodo.16946937
title: Integrated Healthcare Services' Health Statistical Data on Regional Global Budget Payment System in Zhejiang Province, China"
DOI: 10.22002/2sv1m-2rz72
title: Caulobacter delta-CpaF

description: Raw data files of Caulobacter delta-CpaF
description: <strong>Tilt Series Date:</strong> 2019-01-08</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 183.0 eV/Å²,  defocus: -9.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> iMOD</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 28 degrees celsius</p>
<strong>Sample Preparation:</strong> plunge-freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-01-08-78/rawdata/</p>targ29.mrc,  Tilt Series (Pixel Size 0.351 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-01-08-78/rawdata/targ29.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ29_full.rec,  Reconstruction (Pixel Size 1.404 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-01-08-78/3dimage_100926/targ29_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0bpmz-6gs06
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-05</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-38/rawdata/</p>targ38.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-38/rawdata/targ38.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ38_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-38/3dimage_99309/targ38_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yv18c-hec29
title: P. gingivalis wild type

description: Raw data files of P. gingivalis wild type
description: <strong>Tilt Series Date:</strong> 2020-11-06</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown and harvested from plates</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-192/rawdata/</p>PginWT9_191.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-192/rawdata/PginWT9_191.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT9_191_part40_2.rec,  Reconstruction (Pixel Size 1.332 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-192/3dimage_115354/PginWT9_191_part40_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ambbm-0zg58
title: gx3-1_sq2_cell1

description: IT 1s
description: <strong>Tilt Series Date:</strong> 2019-01-18</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter xylinus</p>
<strong>Strain:</strong> JCM 9730</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 68.0 eV/Å²,  defocus: -2.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Culture incubated in shaken conditions at 180rpm - 30C in YFP media + cellulase 0.2g/L</p>
<strong>Sample Preparation:</strong> Broad 100% 22C - Backblotted 5s, glare seen at 2s</p>
  wnj2019-01-18-7_slicer19928.jpg: Cortical sheet?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-01-18-7/rawdata/</p>gx3-1_sq2_cell1.mrc,  Tilt Series (Pixel Size 0.54 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-01-18-7/rawdata/gx3-1_sq2_cell1.mrc"
        > <i class="download icon"></i> Download </a></p>

        gx3-1_sq2_cell1_full.rec,  Reconstruction (Pixel Size 2.16 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-01-18-7/3dimage_100776/gx3-1_sq2_cell1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rd9sx-37v19
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-07-12</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, over-express, from Partick, 4331</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-07-12-12/rawdata/</p>targ09.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-07-12-12/rawdata/targ09.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ09_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-07-12-12/3dimage_100159/targ09_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b2vz9-tqx54
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-08</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-08-20/rawdata/</p>targ21.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-08-20/rawdata/targ21.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ21_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-08-20/3dimage_99533/targ21_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g7rg0-6zv28
title: ec1-1_sq1_cell2

description: IT 1.1s
description: <strong>Tilt Series Date:</strong> 2018-06-28</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 1094</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 165.0 eV/Å²,  defocus: -4.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 3ml culture 37deg, 2hours, OD 1</p>
<strong>Sample Preparation:</strong> On Yiwei's advice: 4uL drop, BF -6, BT 4s, both sides, 100% hum. A lot of the bacterias were blotted away</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-06-28-16/rawdata/</p>sq1_cell2.mrc,  Tilt Series (Pixel Size 0.54 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-06-28-16/rawdata/sq1_cell2.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq1_cell2_full.rec,  Reconstruction (Pixel Size 2.16 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-06-28-16/3dimage_93076/sq1_cell2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rwdmk-bar25
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-79/rawdata/</p>caulobacter_na1000_wt_080_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-79/rawdata/caulobacter_na1000_wt_080_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_080_af.mrc.mdoc,  813.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-79/rawdata/caulobacter_na1000_wt_080_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3tnw6-bct86
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-17</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-7/rawdata/</p>targ07.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-7/rawdata/targ07.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ07_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-7/3dimage_99541/targ07_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d6dyn-d6k97
title: Legionella delta DotO

description: Strain from Yale
description: <strong>Tilt Series Date:</strong> 2019-01-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jun Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase, broad vitrobot, R2/2 quantifoil 200 mesh copper grid</p>
<strong>Sample Preparation:</strong> 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-52/rawdata/</p>tilt_101.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-52/rawdata/tilt_101.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_101_full.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-52/3dimage_101082/tilt_101_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bh33z-sh651
title: gh5-4_sq1_cell4

description: GH5-4, Sq1, IT 1.6s one half and 1s the other
description: <strong>Tilt Series Date:</strong> 2018-04-15</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 38.0°), step: 2°, constant angular increment, dosage: 174.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Shaking 1 day old cultures (24h approx) cultured in 10mL in sealed falcon tubes at 30deg, 2000rpm</p>
<strong>Sample Preparation:</strong> Backside manual blotting 5s</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-15-1/3dimage_92671/</p>gh5-4_sq1_cell4_fakesirt30.rec,  Reconstruction (Pixel Size 1.728 nm), 211.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-15-1/3dimage_92671/gh5-4_sq1_cell4_fakesirt30.rec"
        > <i class="download icon"></i> Download </a></p>

        gh5-4_sq1_cell4.st,  Tilt Series (Pixel Size 0.43 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-15-1/rawdata/gh5-4_sq1_cell4.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4f7es-wc696
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-165/rawdata/</p>caulobacter_na1000_wt_167_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-165/rawdata/caulobacter_na1000_wt_167_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_167_af.mrc.mdoc,  802.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-165/rawdata/caulobacter_na1000_wt_167_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>