cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/kv9vr2jxbm.1
title: C-glycosides analogues of the okadaic acid central fragment exert neuroprotection via restoration of PP2A-phosphatase activity: A rational design of potential drugs for Alzheimer's disease targeting tauopathies

description: These data illustrates the capacitity of a family of C-glycosides to enhance and activate protein phosphatase 2A (PP2A). This enzyme is an important Ser/Thr phosphatase that participates in the regulation of multiple cellular processes, and its deficient activity of PP2A has been involved in the etiology of various diseases.
The C-glycosides presented in this set of data are analogues to the central fragment of the toxin okadaic acid, which selectively inhibits PP2A. Chemical design aimed to turn the pharmalogical activity in a PP2A-activating profile. In order to probe our hypothesis, these compounds were tested in pharmacological models of Alzheimer's disease (AD). Depression of PP2A has been widely corroborated in AD, where the resulting manifestation is the hyperphosphorylation of several PP2A substrates, such as tau protein. Indeed, one of the main histopathological features of AD are neurofibrillary tangles, formed by hyperphosphorylated tau protein.
Therefore, data herein deposited show:
1) recovery of phosphatase activity by the described compounds in a scenario of PP2A depression defined by administration of either okadaic acid or cytostatin (a milder PP2A inhibitor but much more selective), monitored by the pNPP method: files "pNPP vs..."
2) protection of neuroblatoma cultures and cortical neurons exerted by compounds in presence of several toxic stimuli related to neurodegeneration (okadaic acid, cytostatin, rotenone plus oligomycin A or glutamate) measured by MTT method: files "Protection vs ..."
3) effect of the best PP2A-activating compound (10) on the PP2A activity  of two diferent trimeric complexes, namely B55alpha and B56alfa, measured by the malachite green method: file "Malachite of 10 vs OA by Arribas et al"
 4) effect of 10 on the expression of PP2A phosphoprotein substrates in presence of okadaic acid: files "WB of ..."
5) computational docking of compound 10 at its binding site in PP2A-AC dimer: files "Docking of most stable 10 at PP2A"
6) study of the CNS penetration of compound 10 by PAMPA: file "PAMPA of 10 by Arribas et al"
7) in vivo studies of the effect of 10 on the memory deficit exacerbated by LPS in mice, measured by the object recognition test: file "ORT of 10 vs LPS by Arribas et al"
These promising experimental data, centered on profiling the lead compound ITH12711 (10)(X ray data also deposited as cif file and crystal structure report", would validate our rational approach to design new PP2A-activating drugs based on OA central fragment.
DOI: 10.25549/usctheses-ouc11256053
title: Unknown
DOI: 10.17632/4x3wm6gkf6.1
title: Data for - Macroevolutionary decline in mycorrhizal colonization and chemical defence responsiveness to mycorrhization

description: This data corresponds to a greenhouse common garden experiment with 24 species of Plantago. Half of the plants were mycorrhized with a community of four species of AMF, while the other half was left non-mycorrhized. Several functional and chemical traits were measured on the plants after >8  weeks of growth.
DOI: 10.25549/usctheses-ouc11256262
title: Unknown
DOI: 10.25549/usctheses-ouc11256267
title: Unknown
DOI: 10.25549/usctheses-ouc11256268
title: Unknown
DOI: 10.17632/9n8r33bzkj.1
title: Automated classification of hidradenitis suppurativa disease severity by convolutional neural network analyses using clinical images – a prospective study, Supplements

description: Supplemental figures for Manuscript "Automated classification of hidradenitis suppurativa disease severity by convolutional neural network analyses using clinical images – a prospective study."

Figure legends:

Supplemental Fig 1. Individual Typology Angle (ITA°) map. According to their ITA°, individuals were assigned to one of seven skin classification categories. To see if the difference between actual Fitzpatrick (Expert) and ITA phototype (Scarletred®Vision) are significant, a Mann Whitney U Test was conducted (n=149, significance level 5%). The null hypothesis for this test states that there will be no significant difference between the two groups. The test resulted in a p-value of ≈ 0.09 (> 0.05). Therefore, there is no significant difference between the two groups which supports Scarletred®Vision robustness to compute the ITA° related phototype. 

Supplemental Fig 2. Images were signal augmented and analyzed by measuring SEV*, L*, +a* and +b* values. Clear trends were observed in the SEV*_meandt-refdt and +a*_meandt-refdt (dark green squares, p<0.001), indicating the importance of the SEV* for classification and lesion detection also in this study 13.

Supplemental Fig 3. The CNN predicted score aligned with changes of the IHS4, reflecting HS specific dynamics.
DOI: 10.25549/usctheses-ouc11255912
title: Unknown
DOI: 10.25549/usctheses-ouc11255913
title: Unknown
DOI: 10.25549/usctheses-ouc11256051
title: Unknown
DOI: 10.25549/usctheses-ouc11256307
title: Unknown
DOI: 10.17632/xtzgzy997p.1
title: Risk Factors for Stunting in Toddlers in Developing Countries

description: Introduction; Stunting in toddlers is one of the nutritional problems in the world, especially in developing countries. As many as 21.3% or around 144 million toddlers in the world are stunted, more than half of the world's stunted toddlers are from Asia, and more than two-fifths live in Africa. Stunting is a serious threat to the existence of children as the next generation of a nation.
Purpose; This study analyzed the risk factors associated with the incidence of stunting in toddlers in developing countries using a systematic literature review and meta-analysis.
Method; Literature search using online journal portals namely ProQuest, PubMed, and Garuda. The journal used in this study is a journal with a maximum reputation of Q2 and sinta 2, in the period 2010-2020, with a cross-sectional and case-control study design.
Results; Based on the search results, seven kinds of literature were obtained, and conducted by systematic literature review was.
Conclusion; The results of the meta-analysis of risk factors related to the incidence of stunting in toddlers in developing countries are birth weight and exclusive breastfeeding
DOI: 10.25549/usctheses-ouc11256113
title: Unknown
DOI: 10.25549/usctheses-ouc11256122
title: Unknown
DOI: 10.25549/usctheses-ouc11256123
title: Unknown
DOI: 10.17632/4x77nynfnx.1
title: Data for "Soil self-cleaning capacity: Removal of organic compounds during sub-surface irrigation"

description: These files contain the data used in the manuscript: "Soil self-cleaning capacity: Removal of organic compounds during sub-surface irrigation".  We assessed the fate of 133 contaminants of emerging concern relevant for effluents such as herbicides, industrial chemicals, pharmaceuticals, personal care products, and their transformation products in a real-scale sub-surface irrigation system fed by sewage treatment plant (STP) effluent related to their physicochemical properties (half-life range of 0.4 to 1194 days and log Dow range of -2.77 to 5.67). In our study we were able to capture the extremely dry year of 2018 to elucidate the maximum influence of the STP effluent reuse on croplands. We show that sub-surface irrigation significantly removes most contaminants of emerging concern but care must be taken to monitor the contaminants of emerging concern that are (moderately) hydrophobic as these significantly accumulate around infiltration pipes and show no significant removal in the rhizosphere. This removal (either positive or negative) is highly dependent on the persistency-mobility class of a compound, the seasonal drought conditions, and the compartment (rhizosphere, shallow and deep groundwater) of the sub-surface irrigation system. Overall 89/133 emerging organic contaminants were detected in our study. The data consist of one excel file with the concentrations of these emerging organic contaminants (mean ± sd of three technical replicates) in time depth and space. The excel file also has information about the pH, EC and chloride-bromide ratio measured for each sampling location in time and depth.
DOI: 10.17632/4x77nynfnx.2
title: Data for "Soil self-cleaning capacity: Removal of organic compounds during sub-surface irrigation"

description: These files contain the data used in the manuscript: "Soil self-cleaning capacity: Removal of organic compounds during sub-surface irrigation".  We assessed the fate of 133 contaminants of emerging concern relevant for effluents such as herbicides, industrial chemicals, pharmaceuticals, personal care products, and their transformation products in a real-scale sub-surface irrigation system fed by sewage treatment plant (STP) effluent related to their physicochemical properties (half-life range of 0.4 to 1194 days and log Dow range of -2.77 to 5.67). In our study we were able to capture the extremely dry year of 2018 to elucidate the maximum influence of the STP effluent reuse on croplands. We show that sub-surface irrigation significantly removes most contaminants of emerging concern but care must be taken to monitor the contaminants of emerging concern that are (moderately) hydrophobic as these significantly accumulate around infiltration pipes and show no significant removal in the rhizosphere. This removal (either positive or negative) is highly dependent on the persistency-mobility class of a compound, the seasonal drought conditions, and the compartment (rhizosphere, shallow and deep groundwater) of the sub-surface irrigation system. Overall 89/133 emerging organic contaminants were detected in our study. The data consist of one excel file with the concentrations of these emerging organic contaminants (mean ± sd of three technical replicates) in time depth and space. The excel file also has information about the pH, EC and chloride-bromide ratio measured for each sampling location in time and depth.
DOI: 10.25549/usctheses-ouc11256131
title: Unknown
DOI: 10.25549/usctheses-ouc11255927
title: Unknown
DOI: 10.25549/usctheses-ouc11255938
title: Unknown
DOI: 10.25549/usctheses-ouc11255951
title: Unknown
DOI: 10.17632/9yjpwnvmb8.1
title: Mindfulness & Substance Abuse

description: SPSS & AMOS Outputs
DOI: 10.25549/usctheses-ouc11256145
title: Unknown
DOI: 10.17632/s2xx9wvhy5.1
title: Immunometabolic profiling of juvenile idiopathic arthritis patients reveals arginase-independent blockade of synovial T cells

description: Background: Juvenile idiopathic arthritis (JIA) is an inflammatory rheumatic disorder featuring inflamed joints and degradation of synovial tissue. Autoimmune complications are seen in 30-50% of JIA patients who develop anti-nuclear antibodies (ANA), which can result in uveitis and vision loss. Although systemic pro-inflammatory signals have been implicated in JIA pathogenesis, relatively little is known about the local immunometabolic poise in the inflamed synovium itself.
Approach: To shed light into immunopathogenesis of JIA, we collected synovial fluid (SF) from the knee (n = 17) and venous blood (n = 11) of adolescent JIA patients ( n = 16) at 20 individual clinic visits. SF and blood leukocytes were analyzed by flow cytometry, and SF fluid and plasma used for 20-plex mediator quantification and metabolomics. Healthy donor PBMCs were cultured with anti-CD3/CD28 and IL-2 with or without SF to assess the impact of the fluid on T-cell activation.
Results: Compared to blood, SF neutrophils showed increased degranulation, and both SF neutrophils and macrophages had increased surface-bound arginase-1 (Arg1), a myeloid cell-derived T-cell inhibitory enzyme. In addition, the neutrophil: T-cell ratio in SF was higher in ANA-negative patients, suggesting that neutrophil dominance in inflamed JIA joints may impede autoantibody production. In vitro, T cells cultured in SF showed impaired proliferation and upregulation of activation markers CD25 and CD69. Interestingly, T-cell blockade was similar in SF from patients who were neutrophil-high and ANA-negative and those who were neutrophil-low and ANA-positive. Furthermore, levels of arginine and ornithine (substrate and product of Arg1) did not differ in SF from ANA-positive and negative patients. Similarly, levels of tryptophan and kynurenine, the substrate and product of indolamine deoxygenase-1 (IDO-1, another T-cell inhibitory enzyme) did not differ in SF from ANA-positive and negative patients.
Conclusion: Collectively, our findings show that conventional T-cell activation is blocked in SF from JIA patients. Even though ANA-negative JIA patients tend to present with neutrophil- and Arg1-rich SF, T-cell blockade therein is not mediated by immunometabolic routes controlled by Arg1. Future studies should investigate alternative pathways that may control the balance between neutrophils and T cells in the complex joint microenvironment of JIA patients.
DOI: 10.17632/s2xx9wvhy5.2
title: Immunometabolic profiling of juvenile idiopathic arthritis patients reveals arginase-independent blockade of synovial T cells

description: Background: Juvenile idiopathic arthritis (JIA) is an inflammatory rheumatic disorder featuring inflamed joints and degradation of synovial tissue. Autoimmune complications are seen in 30-50% of JIA patients who develop anti-nuclear antibodies (ANA), which can result in uveitis and vision loss. Although systemic pro-inflammatory signals have been implicated in JIA pathogenesis, relatively little is known about the local immunometabolic poise in the inflamed synovium itself.
Approach: To shed light into immunopathogenesis of JIA, we collected synovial fluid (SF) from the knee (n = 17) and venous blood (n = 11) of adolescent JIA patients ( n = 16) at 20 individual clinic visits. SF and blood leukocytes were analyzed by flow cytometry, and SF fluid and plasma used for 20-plex mediator quantification and metabolomics. Healthy donor PBMCs were cultured with anti-CD3/CD28 and IL-2 with or without SF to assess the impact of the fluid on T-cell activation.
Results: Compared to blood, SF neutrophils showed increased degranulation, and both SF neutrophils and macrophages had increased surface-bound arginase-1 (Arg1), a myeloid cell-derived T-cell inhibitory enzyme. In addition, the neutrophil: T-cell ratio in SF was higher in ANA-negative patients, suggesting that neutrophil dominance in inflamed JIA joints may impede autoantibody production. In vitro, T cells cultured in SF showed impaired proliferation and upregulation of activation markers CD25 and CD69. Interestingly, T-cell blockade was similar in SF from patients who were neutrophil-high and ANA-negative and those who were neutrophil-low and ANA-positive. Furthermore, levels of arginine and ornithine (substrate and product of Arg1) did not differ in SF from ANA-positive and negative patients. Similarly, levels of tryptophan and kynurenine, the substrate and product of indolamine deoxygenase-1 (IDO-1, another T-cell inhibitory enzyme) did not differ in SF from ANA-positive and negative patients.
Conclusion: Collectively, our findings show that conventional T-cell activation is blocked in SF from JIA patients. Even though ANA-negative JIA patients tend to present with neutrophil- and Arg1-rich SF, T-cell blockade therein is not mediated by immunometabolic routes controlled by Arg1. Future studies should investigate alternative pathways that may control the balance between neutrophils and T cells in the complex joint microenvironment of JIA patients.
DOI: 10.17632/s2xx9wvhy5.3
title: Immunometabolic analysis of synovial fluid from juvenile idiopathic arthritis patients

description: Juvenile idiopathic arthritis (JIA) is an inflammatory rheumatic disorder. Polymorphonuclear neutrophils (PMNs) are present in JIA synovial fluid (SF), but with variable frequency. Besides, SF PMNs in JIA were previously shown to display high exocytic but low phagocytic and immunoregulatory activities. To further assess whether the degree of SF neutrophilia associated with altered immune responses in JIA, we collected SF and blood from 16 adolescent JIA patients. SF and blood leukocytes were analyzed by flow cytometry. SF and plasma were used for immune mediator quantification and metabolomics. Healthy donor blood T cells were cultured in SF to evaluate its immunoregulatory activities. PMN and T-cell frequencies were bimodal in JIA SF, delineating PMN high / T-cell low (PMNHigh) and PMN low / T-cell high (PMNLow) samples. Pro-inflammatory mediators were increased in SF compared to plasma across patients, and pro- and anti-inflammatory mediators were further elevated in PMNHigh SF. Compared to blood, SF PMNs showed increased exocytosis and PD-1 / PD-L1 expression, and SF PMNs and monocytes/macrophages had increased surface-bound arginase-1. SPADE analysis revealed SF monocyte/macrophage subpopulations coexpressing PD-1 and PD-L1, with higher expression in PMNHigh SF. Healthy donor T cells showed reduced coreceptor expression when stimulated in PMNHigh vs. PMNLow SF. However, amino acid metabolites related to arginase-1 and IDO-1 pathways did not differ between the two groups. Hence, PMN predominance in the SF of a subset of JIA patients is associated with elevated immune mediator concentration and may alter SF monocyte/macrophage phenotype and T-cell activation, without altering immunoregulatory amino acids.
DOI: 10.17632/s2xx9wvhy5.4
title: Immunometabolic analysis of synovial fluid from juvenile idiopathic arthritis patients

description: Juvenile idiopathic arthritis (JIA) is an inflammatory rheumatic disorder. Polymorphonuclear neutrophils (PMNs) are present in JIA synovial fluid (SF), but with variable frequency. Besides, SF PMNs in JIA were previously shown to display high exocytic but low phagocytic and immunoregulatory activities. To further assess whether the degree of SF neutrophilia associated with altered immune responses in JIA, we collected SF and blood from 16 adolescent JIA patients. SF and blood leukocytes were analyzed by flow cytometry. SF and plasma were used for immune mediator quantification and metabolomics. Healthy donor blood T cells were cultured in SF to evaluate its immunoregulatory activities. PMN and T-cell frequencies were bimodal in JIA SF, delineating PMN high / T-cell low (PMNHigh) and PMN low / T-cell high (PMNLow) samples. Pro-inflammatory mediators were increased in SF compared to plasma across patients, and pro- and anti-inflammatory mediators were further elevated in PMNHigh SF. Compared to blood, SF PMNs showed increased exocytosis and PD-1 / PD-L1 expression, and SF PMNs and monocytes/macrophages had increased surface-bound arginase-1. SPADE analysis revealed SF monocyte/macrophage subpopulations coexpressing PD-1 and PD-L1, with higher expression in PMNHigh SF. Healthy donor T cells showed reduced coreceptor expression when stimulated in PMNHigh vs. PMNLow SF. However, amino acid metabolites related to arginase-1 and IDO-1 pathways did not differ between the two groups. Hence, PMN predominance in the SF of a subset of JIA patients is associated with elevated immune mediator concentration and may alter SF monocyte/macrophage phenotype and T-cell activation, without altering immunoregulatory amino acids.
DOI: 10.17632/s2xx9wvhy5.5
title: Immunometabolic analysis of synovial fluid from juvenile idiopathic arthritis patients

description: Juvenile idiopathic arthritis (JIA) is an inflammatory rheumatic disorder. Polymorphonuclear neutrophils (PMNs) are present in JIA synovial fluid (SF), but with variable frequency. Besides, SF PMNs in JIA were previously shown to display high exocytic but low phagocytic and immunoregulatory activities. To further assess whether the degree of SF neutrophilia associated with altered immune responses in JIA, we collected SF and blood from 16 adolescent JIA patients. SF and blood leukocytes were analyzed by flow cytometry. SF and plasma were used for immune mediator quantification and metabolomics. Healthy donor blood T cells were cultured in SF to evaluate its immunoregulatory activities. PMN and T-cell frequencies were bimodal in JIA SF, delineating PMN high / T-cell low (PMNHigh) and PMN low / T-cell high (PMNLow) samples. Pro-inflammatory mediators were increased in SF compared to plasma across patients, and pro- and anti-inflammatory mediators were further elevated in PMNHigh SF. Compared to blood, SF PMNs showed increased exocytosis and PD-1 / PD-L1 expression, and SF PMNs and monocytes/macrophages had increased surface-bound arginase-1. SPADE analysis revealed SF monocyte/macrophage subpopulations coexpressing PD-1 and PD-L1, with higher expression in PMNHigh SF. Healthy donor T cells showed reduced coreceptor expression when stimulated in PMNHigh vs. PMNLow SF. However, amino acid metabolites related to arginase-1 and IDO-1 pathways did not differ between the two groups. Hence, PMN predominance in the SF of a subset of JIA patients is associated with elevated immune mediator concentration and may alter SF monocyte/macrophage phenotype and T-cell activation, without altering immunoregulatory amino acids.
DOI: 10.25549/usctheses-ouc11255967
title: Unknown
DOI: 10.17632/zhdgr86x2h.1
title: A Prospective, Randomized, Blinded and Controlled Comparison Between Picosecond Laser and Q-switched Neodymium Yttrium Aluminum Garnet Laser for Children's Nevus of Ota

description: The aim of this randomized controlled study was to compare the efficacy and adverse effects of picosecond alexandrium and nanosecond Q-switched neodymium yttrium aluminum garnet laser in the treatment of nevus of Ota in children.
DOI: 10.25549/usctheses-ouc11255968
title: Unknown
DOI: 10.25549/usctheses-ouc11255955
title: Unknown
DOI: 10.25549/usctheses-ouc11256312
title: Unknown
DOI: 10.25549/usctheses-ouc11256313
title: Unknown
DOI: 10.17632/s87rp34cgp.1
title: Ice-Pop

description: Study Objective: To evaluate the benefit of ice packs as a supplement to standard pain management following laparoscopic hysterectomy. 
Design: This IRB approved randomized control trial involved patients undergoing laparoscopic hysterectomy (LH) for benign conditions. Subjects were randomized to receive standard enhanced recovery after surgery (ERAS) pain management or standard ERAS plus ice packs.  
Setting: 2 academic tertiary care centers 
Patients: Patients undergoing planned outpatient LH with the minimally invasive gynecologic surgery team between February 2019 and November 2020 were considered.  Patients with chronic pain, current opioid use >1 week, or planned overnight hospitalizations were excluded.  Primary outcome data was available for 51 subjects (24 control, 27 intervention) 
Interventions: Ice packs were placed on the abdomen in the operating room 
Measurements and Main Results: Pain was assessed at multiple time points throughout the study using Visual Analogue Scale (VAS). Narcotic requirement was assessed using morphine milligram equivalent (MME). There was no difference between the groups’ demographic data.  MME requirements were not different between the groups (p=.63). Postoperative day 1 (POD#1) VAS scores were not different (p=.89). 84.8% of subjects felt their pain was controlled.  Subjects that did not feel their pain was controlled did not use more narcotics POD#1 (p=.37), nor did they have higher POD#1 VAS scores (p=.55). 87% of intervention subjects would use ice again and 82.6% would recommend ice to others. There were no adverse events related to ice. All subjects were prescribed 20 tablets oxycodone and averaged 2.9 (SD 3.4) tablets used after discharge. 
Conclusion: Ice packs are an acceptable supplement for postoperative pain control, but do not impact postoperative narcotic usage. Providers caring for postoperative patients should be cognizant of unintentional consequences associated with overprescribing opioids and consider reducing the number prescribed given consistent evidence that patients require less than half the quantity given.
DOI: 10.25549/usctheses-ouc11256315
title: Unknown