DOI: 10.5061/dryad.vmcvdnd0q
title: Data from: The SUbventral-Gland Regulator (SUGR) of nematode virulence

description: All pathogens must tailor their gene expression to their environment.
 Therefore, targeting host:parasite biology that regulates these changes in
 gene expression could open up routes to pathogen control. Here, we show
 that in the plant-parasitic nematode Heterodera
 schachtii, host signals (termed effectostimulins) within plant
 roots activate the master regulator sugr1. SUGR1, then, directly
 binds effector promoters, and orchestrates their production. Effector
 production, in turn, facilitates host entry, releasing more
 effectostimulins. These data show that gene expression during the very
 earliest stages of parasitism is defined by a feed forward loop for host
 entry. Importantly, we demonstrate that blocking SUGR1 blocks parasitism,
 underlining the SUGR1 signalling cascade as a valuable target for crop
 protection. Given that nematodes also parasitise humans and other animals,
 the potential impact is broad: disrupting effector production could, in
 principle, be applied to any pathogen that secrets effectors.
description: Instructions for the generation of transcriptional networks are
 available here: https://github.com/BethMolloy/Effectorome_H_schachtii Instructions for the generation of Y1H original pictures are available in the associated Read me.pdf
description: # Data from: The SUbventral-Gland master Regulator (SUGR) of nematode
 virulence. ## **Transcriptional network files** Transcriptional network
 files for the plant-parasitic cyst nematode *Heterodera schachtii* showing
 correlations in gene expression across the life cycle (Siddique et al.,
 2022) between effector genes (as predicted in Molloy et al., 2024) and
 those endogenous *H. schachtii* transcription factors transcriptionally
 upregulated upon exposure to root extract (Pellegrin and Damm et al.,
 2024). **Description of the data and file structure**
  SUGR_network_0.975.gexf Transcriptional network file showing
 correlations, above a threshold correlation coefficient of 0.975, between
 *H. schachtii* effector genes and root-extract upregulated *H. schachtii*
 transcription factors across the nematode life cycle.
 0975_SUGR_TF_network_supercluster. gephi Transcriptional network file
 allowing nodes to be coloured by various gene attributes such as
 supercluster (as defined by Siddique et al. (2022)), TF vs effector,
 root-extract upregulated effectors etc. The node “level” can also be split
 to show TFs and effectors in different planes, with the height of each TF
 correlated with number of connected effectors. .gephi and .gexf network
 files can be opened in Gephi v0.10, which can be downloaded for free from:
 [https://gephi.org](https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgephi.org%2F&data=05%7C02%7Cse389%40cam.ac.uk%7C914226e87afa4863a37308dcacb7b1bd%7C49a50445bdfa4b79ade3547b4f3986e9%7C1%7C0%7C638575152157010261%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=5jXOtpDohuSDSj%2BreMPKtlrhJlyCScjFQSM84j%2FLpus%3D&reserved=0 "Original URL: https://gephi.org/. Click or tap if you trust this link.").  **Sharing/Access information** \** **Instructions for generating transcriptional networks are available here:  [https://github.com/BethMolloy/Effectorome_H_schachtii](https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2FBethMolloy%2FEffectorome_H_schachtii&data=05%7C02%7Cse389%40cam.ac.uk%7C914226e87afa4863a37308dcacb7b1bd%7C49a50445bdfa4b79ade3547b4f3986e9%7C1%7C0%7C638575152157024291%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=cC8VEyQXRcEpI5N78KTbPQ%2B1tBdVgzX%2Bzlvus6voiyI%3D&reserved=0 "Original URL: https://github.com/BethMolloy/Effectorome_H_schachtii. Click or tap if you trust this link.") and outlined in Molloy et al. (2024). ## **Yeast 1 Hybrid (Y1H) original images** Original pictures of yeast one hybrid screen shown in Figures S4-5. Binding of SUGR-1, SaTFa (SUGR activated Transcription Factor a), and SaTFb (SUGR activated Transcription Factor b) to promoters and promoter halves (proximal (b) and distal (a) halves) of SUGR-1 activated genes. Original pictures also show unpublished results for transcription factor x (not included in Figures S4-5 and the manuscript). The associated "Read me.pdf" contains a description of the layout of each image such that with the name of the file and this layout, the contents of the image can be correctly deduced. **References** \** **S. Siddique, et al., The genome and lifestage-specific transcriptomes of a plant-parasitic nematode and its host reveal susceptibility genes involved in trans-kingdom synthesis of vitamin B5. Nat. Commun. 13, 6190 (2022).  B. Molloy et al., The Origin, Deployment, and Evolution of a Plant-Parasitic Nematode Effectorome. BioRxiv. (2024).  C. Pellegrin and A. Damm et al., The SUbventral-Gland master regulator (SUGR) of nematode virulence. BioRxiv. (2024). ### Version changes 09-Feb-2025. Added a section on Yeast 1 Hybrid (Y1H) original images