DOI: 10.20350/digitalcsic/17759
title: Dopamine enhances multisensory responses in the dorsomedial striatum [Dataset]

description: The folders in this dataset contain the following data: #FOLDER_1: Contains the following data: # Anatomy: Folder containing microscope images: + BDA_Injections: Microscope images of DMS or DLS striatum where somatosensory or visual cortex was injected with Biotin dextrane amine. + Viral_Injections: Microscope images of DMS or DLS striatum where somatosensory or visual cortex was injected with AAV2-EF1a-DIO-tdTomato-WPRE in Tlx3cre (IT) or OE25-cre (PT) transgenic animals; # DA_release: +DA_release: Experiments where dopamine was optogenetically released in the DMS by injecting the virus AAV5-hSyn-FLEX-ChrimsonR-tdTomato in the SNc ("DA_release" folder) and then compared it to the spontaneous activity file of each cell ("Spontaneous_activity" folder) to study how dopamine affects spontaneous activity parameters; + Negative_controls: Negative controls for the DA_release experiments, where the SNc was injected with the virus AAV5-CAG-FLEX-tdTomato instead of the virus that contains the opsin. For each cell, find the protocol of the dopamine release ("Negative" folder) and the spontaneous activity file ("Spontaneous_activity" folder) to compare. -- #FOLDER_2: Contains the following data: # Fiber_photometry_Ach: Data from fiber photometry experiments where acetylcholine was measured when sensory stimulation was performed by injecting AAV5-hSyn-GRAB-ACh3.0-GFP in the dorsolateral striatum (DLS). -- #FOLDER_3 to FOLDER_11: Contains the following data: # Fiber_photometry_Ach: Data from fiber photometry experiments where acetylcholine was measured when sensory stimulation was performed by injecting AAV5-hSyn-GRAB-ACh3.0-GFP in the dorsomedial striatum (DMS). All folders contain all the animals used for the experiments. -- #FOLDER_12: Contains the following data: # Fiber_photometry_dopamine: + Measurement: Data from fiber photometry experiments where dopamine was measured after optogenetic stimulation with AAV5-hSyn-FLEX-ChrimsonR-tdTomato virus injected in the SNc. "DMS" or "SNc" in the file name refers to the measurement location, dorsomedial striatum or substantia nigra pars compacta, respectively; + Negative_controls: Same from above, only difference is that the AAV5-hSyn-FLEX-ChrimsonR-tdTomato virus was the one injected in the SNc to perform experimental controls. -- #FOLDER_13: Contains the following data: # Interneurons: + Cholinergic_Interneurons: Raw recordings of 3 Cholinergic Interneurons. One of them has all the protocols in the same recording (cell 20161024) and the other two have the spontaneous activity protocols used to extract membrane properties ("sp_act") and the protocols were visual stimulation was performed ("optostim") used for visual stimulation examples in supplementary figure 1; + Fast Spiking_Interneurons: Raw recordings of 2 Fast Spiking Interneurons used to extract membrane properties; # Spontaneous_activity: Recordings of 33 MSNs (18 D1-MSNs + 15 D2-MSNs) where MSNs where identified as D1 or D2 with light pulses via optopatcher, and spontaneous activity was used to extract membrane properties. -- #FOLDER_14 and FOLDER_15: Contains the following data: # Sensory_stimulation_optostim: Recordings of 29 MSNs (14 D1-MSNs + 15 D2-MSNs) where sensory stimulation (tactile, visual, tactile+visual) and optogenetic release of dopamine was performed. Animals were injected with AAV5-hSyn-FLEX-ChrimsonR-tdTomato in the SNc. The two folders contain all the animals used for the experiments.
description: The brain continuously integrates information from multiple sensory modalities to generate adaptive responses, yet, the underlying neural substrates remain elusive. In the dorsomedial striatum, medium spiny neurons (MSNs) respond to both tactile and visual stimuli, which arrive with distinct latencies. This temporal disparity points to a dynamic integration mechanism operating at the cellular or circuit level. To investigate this, we examined how dopamine influences tactile and visual responses, as well as bimodal integration in identified MSNs, using in vivo optopatch-clamp recordings. Our results reveal that dopamine enhances bimodal responses in direct pathway MSNs, by accelerating visual responses and facilitating sensory convergence. To clarify how this dopamine-dependent process operates, we combined anatomical, functional, and computational data, indicating that it relies on the disinhibition of direct pathway MSNs via a cell-type-specific corticostriatal circuit. These findings uncover a mechanism by which dopamine promotes temporal alignment and integration of sensory inputs in the striatum.
description: File List: Anatomy, DA_release, Fiber_photometry_Ach, Fiber_photometry_dopamine, Interneurons, Sensory_stimulation_optostim, Spontaneous_activity.