cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7519301.v1
title: Biology and morphology of the shoot fly (Diptera: Lonchaeidae)

description: The aim of this study was to determine biological and morphological patterns of the male shoot fly, Neosilba perezi(Diptera: Lonchaeidae). Under laboratory conditions, the incubation period of eggs took two days, on average (viability of eggs: 55.5%). Bred on an artificial diet, based on cassava flour, the mean time of development of the newly hatched larvae to pupa formation occurred in 15 days, and the average pupal period took 23 days. Finally, the biological cycle, from egg to adult, lasted approximately 40 days. The eggs are white colored, have an elliptical form and are about 960 ± 5.30 µm long and 290 ± 1.30 µm large in the central region; the vermiform larvae, third instar, are milky white and are ± 10 mm long (3rd instar).
DOI: 10.6084/m9.figshare.7518401
title: In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid

description: The control of enterotoxemia caused by the epsilon toxin, produced by Clostridium perfringenstype D, is based on vaccination with epsilon toxoid. The potency test for this immunogen is conducted using seroneutralization in mice. Here, an in vitro test for detection of neutralizing antibodies with Madin-Darby Canine Kidney (MDCK) cells was standardized in order to study alternative methodologies for the potency test. Titers observed in the in vivo and in vitro seroneutralization tests had a correlation of 99.73%.
DOI: 10.6084/m9.figshare.7518401.v1
title: In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid

description: The control of enterotoxemia caused by the epsilon toxin, produced by Clostridium perfringenstype D, is based on vaccination with epsilon toxoid. The potency test for this immunogen is conducted using seroneutralization in mice. Here, an in vitro test for detection of neutralizing antibodies with Madin-Darby Canine Kidney (MDCK) cells was standardized in order to study alternative methodologies for the potency test. Titers observed in the in vivo and in vitro seroneutralization tests had a correlation of 99.73%.
DOI: 10.6084/m9.figshare.7507694
title: Survey of Enterobacteriaceae in domestic ducks (Cairina moschata) from properties located in four cities of the state of Ceará, Brazil

description: This study aimed to isolate and identify members of the Enterobacteriaceae that were present in domestic ducks (Cairina moschata) from properties located in four cities in the State of Ceará, Brazil. Therefore, 65 stool samples and 47 cloacal swabs were collected from farms located in the following cities: Fortaleza, Boa Água, Eusébio and Cascavel. The samples were submitted to bacteriological processing. In pre-enrichment, all of the stool and swab samples were cultured in buffered peptone water 0.1%. For selective enrichment, aliquots from the tubes of the prior step after incubation were transferred to tubes containing Rappaport-Vassiliadis and Selenite Cystine broths. Plates with MacConkey and Brilliant Green agars were streaked with the content from the enrichment tubes after incubation. Colonies were chosen based on their morphological characteristics for the biochemical tests (TSI: Triple-Sugar-Iron; LIA: Lysine-Iron-Agar; and SIM: Sulfide-indole-motility). The bacteria were identified based on their biochemical characteristics. The mostly isolated bacteria were Citrobactersp., Proteussp., and Enterobactersp. In a lower frequency, isolated enterobacteria were Klebsiellasp., Hafniasp., Escherichia coli, Edwardsiellasp., Providenciasp. and Serratiasp. With the methodology applied, no Salmonellawas isolated from the evaluated ducks, which is a genus commonly associated with this avian species; however the microbiota were composed by the main enterobacteria that are common to other species of birds, some of which are potentially pathogenic both to animals and humans.
DOI: 10.6084/m9.figshare.7507694.v1
title: Survey of Enterobacteriaceae in domestic ducks (Cairina moschata) from properties located in four cities of the state of Ceará, Brazil

description: This study aimed to isolate and identify members of the Enterobacteriaceae that were present in domestic ducks (Cairina moschata) from properties located in four cities in the State of Ceará, Brazil. Therefore, 65 stool samples and 47 cloacal swabs were collected from farms located in the following cities: Fortaleza, Boa Água, Eusébio and Cascavel. The samples were submitted to bacteriological processing. In pre-enrichment, all of the stool and swab samples were cultured in buffered peptone water 0.1%. For selective enrichment, aliquots from the tubes of the prior step after incubation were transferred to tubes containing Rappaport-Vassiliadis and Selenite Cystine broths. Plates with MacConkey and Brilliant Green agars were streaked with the content from the enrichment tubes after incubation. Colonies were chosen based on their morphological characteristics for the biochemical tests (TSI: Triple-Sugar-Iron; LIA: Lysine-Iron-Agar; and SIM: Sulfide-indole-motility). The bacteria were identified based on their biochemical characteristics. The mostly isolated bacteria were Citrobactersp., Proteussp., and Enterobactersp. In a lower frequency, isolated enterobacteria were Klebsiellasp., Hafniasp., Escherichia coli, Edwardsiellasp., Providenciasp. and Serratiasp. With the methodology applied, no Salmonellawas isolated from the evaluated ducks, which is a genus commonly associated with this avian species; however the microbiota were composed by the main enterobacteria that are common to other species of birds, some of which are potentially pathogenic both to animals and humans.
DOI: 10.6084/m9.figshare.7517132
title: Effects of cold storage on the quality parameters of goat milk

description: The small-scale goat milk production requires its storage, and such operation is allowed by Brazilian laws. This study aimed at evaluating the cooling and freezing effects on physical-chemical and microbiological characteristics of goat milk. However, no variability was verified in average acidity, alcohol stability, density, fat protein, total solids and lactose values after cold storage. Heat instability was not observed in any of the analyzed samples. There was a decreasing tendency in the somatic cell count (SCC) of frozen milk in comparison to fresh and refrigerated milk. The quantification (NMP) of total and thermo-tolerant coliforms varies from &lt; 3 (no growth) to &gt; 1,100 UFC/mL, regardless of the storage method. However, a slightly higher count (p &lt; 0.0001) was observed for total and thermo-tolerant coliforms in the samples kept under refrigeration. In the lacto-fermentation test, digested, flocculated and grooved clots were observed without establishing a correlation between them and the bacterial count. SCC and microbial count are the criteria adopted when paying for the quality of goat milk, and the storage temperature may affect these criteria.
DOI: 10.6084/m9.figshare.7517132.v1
title: Effects of cold storage on the quality parameters of goat milk

description: The small-scale goat milk production requires its storage, and such operation is allowed by Brazilian laws. This study aimed at evaluating the cooling and freezing effects on physical-chemical and microbiological characteristics of goat milk. However, no variability was verified in average acidity, alcohol stability, density, fat protein, total solids and lactose values after cold storage. Heat instability was not observed in any of the analyzed samples. There was a decreasing tendency in the somatic cell count (SCC) of frozen milk in comparison to fresh and refrigerated milk. The quantification (NMP) of total and thermo-tolerant coliforms varies from &lt; 3 (no growth) to &gt; 1,100 UFC/mL, regardless of the storage method. However, a slightly higher count (p &lt; 0.0001) was observed for total and thermo-tolerant coliforms in the samples kept under refrigeration. In the lacto-fermentation test, digested, flocculated and grooved clots were observed without establishing a correlation between them and the bacterial count. SCC and microbial count are the criteria adopted when paying for the quality of goat milk, and the storage temperature may affect these criteria.
DOI: 10.6084/m9.figshare.7514966
title: Microbiological quality of the fish saw (Scomberomorus brasiliensis) and the ice used for its conservation

description: This study aimed at evaluating the microbiological quality of the saw fish (S. brasiliensis) landed in the city of Raposa (MA) and the ice used in its conservation. Samples of ice and saw fish were subjected to analysis to determine the Most Probable Number (MPN) of coliforms, thermo-tolerants and Escherichia coli. Fish samples were also tested for Staphylococcus coagulase positive, Salmonellaspp. and Aeromonas spp. Fishermen were given a questionnaire with 20 questions concerning the handling of the fish. The ice samples showed unsatisfactory microbiological quality. The saw fish was contaminated with A. hydrophila, which means it could convey this agent to be consumed by the population.
DOI: 10.6084/m9.figshare.7514966.v1
title: Microbiological quality of the fish saw (Scomberomorus brasiliensis) and the ice used for its conservation

description: This study aimed at evaluating the microbiological quality of the saw fish (S. brasiliensis) landed in the city of Raposa (MA) and the ice used in its conservation. Samples of ice and saw fish were subjected to analysis to determine the Most Probable Number (MPN) of coliforms, thermo-tolerants and Escherichia coli. Fish samples were also tested for Staphylococcus coagulase positive, Salmonellaspp. and Aeromonas spp. Fishermen were given a questionnaire with 20 questions concerning the handling of the fish. The ice samples showed unsatisfactory microbiological quality. The saw fish was contaminated with A. hydrophila, which means it could convey this agent to be consumed by the population.
DOI: 10.6084/m9.figshare.7514945.v1
title: Serology and patterns of antimicrobial susceptibility in Escherichia coli isolates from pay-to-fish ponds

description: The occurrence of Escherichiacoli (EPEC, EIEC and O157) in water and fish (skin, gut and muscle) in pay-to-fish ponds of the micro bay of Córrego Rico, in Jaboticabal (SP), was assessed. One hundred and fifteen strains of E. coli were isolated, and 49 (43%) were serogrouped as EPEC. The most common serogroups were O125, O126 and O158. Among the tested samples, 60 (52%) showed simultaneous resistance to two antimicrobials. A correspondence analysis was performed to assess possible correlations involving the site of isolation, serogroups and multi-resistance. The results of this analysis showed that the muscle was less correlated with the the other factors. However, the isolation of EPEC serogroups in this study demonstrates a risk to public health.
DOI: 10.6084/m9.figshare.7514945
title: Serology and patterns of antimicrobial susceptibility in Escherichia coli isolates from pay-to-fish ponds

description: The occurrence of Escherichiacoli (EPEC, EIEC and O157) in water and fish (skin, gut and muscle) in pay-to-fish ponds of the micro bay of Córrego Rico, in Jaboticabal (SP), was assessed. One hundred and fifteen strains of E. coli were isolated, and 49 (43%) were serogrouped as EPEC. The most common serogroups were O125, O126 and O158. Among the tested samples, 60 (52%) showed simultaneous resistance to two antimicrobials. A correspondence analysis was performed to assess possible correlations involving the site of isolation, serogroups and multi-resistance. The results of this analysis showed that the muscle was less correlated with the the other factors. However, the isolation of EPEC serogroups in this study demonstrates a risk to public health.
DOI: 10.6084/m9.figshare.7508933
title: Parasitic mycoflora of sclerotia of Sclerotinia sclerotiorum isolated from soils of the western frontier of the state of Rio Grande do Sul, Brazil

description: The aim of this study was to identify the diversity of the parasitic mycoflora and to identify the necessary time until the beginning of the parasitism of sclerotia of Sclerotinia sclerotiorum in soils of the western frontier of the state of Rio Grande do Sul (RS), Brazil. Agricultural and non agricultural soils of five locations in the western frontier of Rio Grande do Sul were collected. The fungi isolation was performed by testing baits, and baits were the sclerotia of the phytopathogen, which remained buried in the soil for 15, 30 and 60 days. The fungi were identified at the genus level. The following fungal genera were found parasitizing sclerotia: Trichoderma, Penicillium, Aspergillus, Fusarium, Pythium (Cromista) Clonostachys rosea (Gliocladium), Chaetomium, Curvularia, Phytophthora (Cromista), Rhizopus, Lichtheimia (Absidia), Mucor, Acremonium, Periconiaand Isaria. It was observed that the sclerotia were parasitized by fungi until 15 days after they had been buried. And after this period, only the frequency of occurrence of fungi has changed. It was concluded that the fungal genera Isaria, Periconia, Acremonium, Lichtheimia (Absidia), Phytophthora, Curvulariaand Chaetomium are found both in agricultural and non-agricultural soils on the western frontier of RS, but they were not previously cited in other works. There is diversity of parasitic fungi of sclerotia of S. sclerotiorum in the soils of the western frontier of the RS, which can be used in experiments to compose biological control programs for this pathogen. The sclerotia are parasitized by fungi until 15 days after being buried underground.
DOI: 10.6084/m9.figshare.7508933.v1
title: Parasitic mycoflora of sclerotia of Sclerotinia sclerotiorum isolated from soils of the western frontier of the state of Rio Grande do Sul, Brazil

description: The aim of this study was to identify the diversity of the parasitic mycoflora and to identify the necessary time until the beginning of the parasitism of sclerotia of Sclerotinia sclerotiorum in soils of the western frontier of the state of Rio Grande do Sul (RS), Brazil. Agricultural and non agricultural soils of five locations in the western frontier of Rio Grande do Sul were collected. The fungi isolation was performed by testing baits, and baits were the sclerotia of the phytopathogen, which remained buried in the soil for 15, 30 and 60 days. The fungi were identified at the genus level. The following fungal genera were found parasitizing sclerotia: Trichoderma, Penicillium, Aspergillus, Fusarium, Pythium (Cromista) Clonostachys rosea (Gliocladium), Chaetomium, Curvularia, Phytophthora (Cromista), Rhizopus, Lichtheimia (Absidia), Mucor, Acremonium, Periconiaand Isaria. It was observed that the sclerotia were parasitized by fungi until 15 days after they had been buried. And after this period, only the frequency of occurrence of fungi has changed. It was concluded that the fungal genera Isaria, Periconia, Acremonium, Lichtheimia (Absidia), Phytophthora, Curvulariaand Chaetomium are found both in agricultural and non-agricultural soils on the western frontier of RS, but they were not previously cited in other works. There is diversity of parasitic fungi of sclerotia of S. sclerotiorum in the soils of the western frontier of the RS, which can be used in experiments to compose biological control programs for this pathogen. The sclerotia are parasitized by fungi until 15 days after being buried underground.
DOI: 10.6084/m9.figshare.7508996.v1
title: Effect of aqueous plant extracts on the fall armyworm

description: Spodoptera frugiperda, fall armyworm, is considered as one of the main pests of corn crops. As an alternative measure to pesticides, the use of insecticidal plants, aside from protecting the environment, reduces production costs and becomes a practice of sustainable agriculture. Thus, the objective of this work was to verify the insecticidal action of aqueous leaf extracts of Eucaplyptus robusta,Mikania laevigata and leaves and fruits of Capsicum baccatum, when incorporated into artificial diet for Spodoptera frugiperda, maintained under laboratory conditions (26 ± 2°C, relative humidity of 60 ± 5% and photoperiod of 16 hours). Larval stage duration, daily mortality, weight of larvae on the 10th day and of pupae after 24 hours were verified, as well as daily mortality of larvae. It was observed that the extracts of E. robustaand leaves of C.baccatum showed higher mortality and lower weight in the larval stage, and are promising for field tests to control this pest.
DOI: 10.6084/m9.figshare.7508999
title: Termites in residences of a neighbourhood in Cáceres, Mato Grosso

description: The objective of this study was to evaluate the abundance of termites and the perception of residents about those insects as pests. This study was conducted inside and in the surroundings of four houses in the neighbourhood Jardim Paraíso, in Cáceres, Mato Grosso. Inspections were carried out in March, May, August and November, 2004 and February. 2005, by manual collection (inside the houses and on trees of the backyards). Questionnaires were used to check the opinions of the residents about these insects. Four species were identified: Nasutitermes coxipoensis (48.2%), Nasutitermes sp. (31%), Microcerotermes strunckii (7%) and Heterotermes tenuis (13.7%). The genera (χ2 = 7.880; GL = 8; α = 0.445) and the species (χ2 = 20.542; GL = 12; α = 0.058) occurred in similar proportions. No significant relation was found between the collection periods and the abundance of the insects. In this study, 50% of the residents did not consider the termites as pests, probably because in most cases (56.4%) termites were found only in the trees. Due to the small number of investigated houses, further studies are necessary for a better understanding of the termite incidence.
DOI: 10.6084/m9.figshare.7508999.v1
title: Termites in residences of a neighbourhood in Cáceres, Mato Grosso

description: The objective of this study was to evaluate the abundance of termites and the perception of residents about those insects as pests. This study was conducted inside and in the surroundings of four houses in the neighbourhood Jardim Paraíso, in Cáceres, Mato Grosso. Inspections were carried out in March, May, August and November, 2004 and February. 2005, by manual collection (inside the houses and on trees of the backyards). Questionnaires were used to check the opinions of the residents about these insects. Four species were identified: Nasutitermes coxipoensis (48.2%), Nasutitermes sp. (31%), Microcerotermes strunckii (7%) and Heterotermes tenuis (13.7%). The genera (χ2 = 7.880; GL = 8; α = 0.445) and the species (χ2 = 20.542; GL = 12; α = 0.058) occurred in similar proportions. No significant relation was found between the collection periods and the abundance of the insects. In this study, 50% of the residents did not consider the termites as pests, probably because in most cases (56.4%) termites were found only in the trees. Due to the small number of investigated houses, further studies are necessary for a better understanding of the termite incidence.
DOI: 10.6084/m9.figshare.7508996
title: Effect of aqueous plant extracts on the fall armyworm

description: Spodoptera frugiperda, fall armyworm, is considered as one of the main pests of corn crops. As an alternative measure to pesticides, the use of insecticidal plants, aside from protecting the environment, reduces production costs and becomes a practice of sustainable agriculture. Thus, the objective of this work was to verify the insecticidal action of aqueous leaf extracts of Eucaplyptus robusta,Mikania laevigata and leaves and fruits of Capsicum baccatum, when incorporated into artificial diet for Spodoptera frugiperda, maintained under laboratory conditions (26 ± 2°C, relative humidity of 60 ± 5% and photoperiod of 16 hours). Larval stage duration, daily mortality, weight of larvae on the 10th day and of pupae after 24 hours were verified, as well as daily mortality of larvae. It was observed that the extracts of E. robustaand leaves of C.baccatum showed higher mortality and lower weight in the larval stage, and are promising for field tests to control this pest.
DOI: 10.6084/m9.figshare.7518461
title: Occurrence of antibodies anti-Brucella ovis in ovine with history of reproductive disorders in São Paulo, Brazil

description: Based upon a history of reproductive disorders, a serological survey and attempts to isolate Brucella ovis were performed in 28 farms in the State of São Paulo, in a total of 294 sheep. The sera sampled on the occasion of the visit to the farms were subject to complement fixation. During clinical examination, samples of several suspicious biological materials were collected for further bacterial culture in Brucella agar medium, in a total of 16 aborted foetuses, one uterus, one placenta, 13 uterine discharge, six vaginal swabs, 17 semen samples and three preputial swabs. The proportion of sheep with a history of reproductive disorders and serum reactive for B. ovis was of 1.7% (5/294), including one ram and four ewes, with titres ranging from 800 IU to 1600 IU. Four of the 28 sheep herds surveyed had serum reactive animals (14,3%). All cultures of biological samples were negative, including semen and swabs from the reproductive organs of a Texel ram, positive in two complement fixation tests, six months apart. In the sheep herds surveyed in the State of São Paulo, it was not possible to relate the reproductive disorders to the positive serology for B. ovis, however, foci were detected, representing a risk factor for bacterial dissemination in the herds.
DOI: 10.6084/m9.figshare.7518461.v1
title: Occurrence of antibodies anti-Brucella ovis in ovine with history of reproductive disorders in São Paulo, Brazil

description: Based upon a history of reproductive disorders, a serological survey and attempts to isolate Brucella ovis were performed in 28 farms in the State of São Paulo, in a total of 294 sheep. The sera sampled on the occasion of the visit to the farms were subject to complement fixation. During clinical examination, samples of several suspicious biological materials were collected for further bacterial culture in Brucella agar medium, in a total of 16 aborted foetuses, one uterus, one placenta, 13 uterine discharge, six vaginal swabs, 17 semen samples and three preputial swabs. The proportion of sheep with a history of reproductive disorders and serum reactive for B. ovis was of 1.7% (5/294), including one ram and four ewes, with titres ranging from 800 IU to 1600 IU. Four of the 28 sheep herds surveyed had serum reactive animals (14,3%). All cultures of biological samples were negative, including semen and swabs from the reproductive organs of a Texel ram, positive in two complement fixation tests, six months apart. In the sheep herds surveyed in the State of São Paulo, it was not possible to relate the reproductive disorders to the positive serology for B. ovis, however, foci were detected, representing a risk factor for bacterial dissemination in the herds.