DOI: 10.22002/9xdbt-f8169
title: NR1769pKM101conjFM433

description: skinny ecoli conjugation with FM433 ecoli
description: <strong>Tilt Series Date:</strong> 2014-10-25</p>
 
<strong>Data Taken By:</strong> Elitza Tocheva</p>
 
<strong>Species / Specimen:</strong> Escherichia coli</p>
 
<strong>Strain:</strong> NR1769 pKM101</p>
 
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å², defocus: -10.0 μm, magnification: 18000x. </p>
 
<strong>Microscope:</strong> Caltech Polara</p>
 
<strong>Acquisition Software:</strong> UCSFTomo</p>
 
<strong>Upload Method:</strong> reupload_downloaded</p>
 
<strong>Processing Software Used:</strong> IMOD</p>
 
<strong>Collaborators and Roles:</strong> Christian Baron</p>
 
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown on a gold grid in a drop of 1:1 mix of bacteria for 30min</p>
 
<strong>Sample Preparation:</strong> Vitrobot</p>
 
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/rawdata/</p>
 
NRpKMplusFM433_conj1_18K0005.mrc, Tilt Series (Pixel Size 0.63 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/rawdata/NRpKMplusFM433_conj1_18K0005.mrc" > <i class="download icon"></i> Download </a>
</p>
 
 NRpKMplusFM433_conj1_18K0005.rec, Reconstruction (Pixel Size 0.0 nm), 2.0 GB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/3dimage_49671/NRpKMplusFM433_conj1_18K0005.rec" > <i class="download icon"></i> Download </a>
</p>
 
 slicer000.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12271/slicer000.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer001.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12272/slicer001.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer002.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12273/slicer002.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer003.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12274/slicer003.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer004.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12275/slicer004.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer005.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12276/slicer005.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer006.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12277/slicer006.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer007.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12278/slicer007.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer008.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12279/slicer008.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer009.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12280/slicer009.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer010.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12281/slicer010.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer011.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12282/slicer011.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer012.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12283/slicer012.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer013.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12284/slicer013.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer014.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12285/slicer014.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer015.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12286/slicer015.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer016.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12287/slicer016.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer017.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12288/slicer017.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer018.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12289/slicer018.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer019.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12290/slicer019.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer020.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12291/slicer020.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer021.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12292/slicer021.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer022.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12293/slicer022.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer023.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12294/slicer023.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer024.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12295/slicer024.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer025.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12296/slicer025.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer026.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12297/slicer026.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer027.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-10/file_12298/slicer027.tif" > <i class="download icon"></i> Download </a>
</p>