DOI: 10.5061/dryad.0rxwdbsc1
title: Olfactory receptor genes expression in Tuta absoluta: A pathway to targeted control strategies

description: The tomato leaf miner, Tuta absoluta (Meyrick), is a major agricultural
 pest with a highly specialized olfactory system that governs host
 localization, mate selection, and oviposition. Despite the critical role
 of olfaction in its invasive success, the genome-wide expression of
 olfactory receptors (ORs) remains poorly understood. This study employs
 RNA sequencing and transcriptomic analysis to characterize the
 differential expression of ORs, ionotropic receptors (IRs), and gustatory
 receptors (GRs) across sensory tissues (antennae vs. maxillary palps), sex
 (male vs. female), and mating status (virgin vs. mated).These data
 represents raw and assembled transcriptome sequences for 
 functional annotation to identify a diverse repertoire of chemosensory
 receptors in the chemosensory tissues of T. absoluta. By integrating
 transcriptomic insights with ecological and behavioral contexts, this
 study provides a foundation for novel, olfaction-based pest management
 strategies against T. absoluta.
description: # Olfactory receptor genes expression in Tuta absoluta: A pathway to
 targeted control strategies Dataset DOI:
 [10.5061/dryad.0rxwdbsc1](10.5061/dryad.0rxwdbsc1) ## Description of the
 data and file structure **#Transcriptomic Dataset of Tuta absoluta
 Olfactory Tissues**\ **Dataset Overview** This dataset contains raw
 sequencing reads (FASTQ files) and Trinity-assembled transcriptomes from
 eight samples of insect sensory tissues (antennae and maxillary palps)
 across sexes (male/female) and mating statuses (virgin/mated). The data
 were generated to investigate differential expression of olfactory
 receptors and other chemosensory genes associated with host-seeking,
 mating, and reproductive behaviors. ### **Dataset Structure** #### **1.
 Raw Data** * **Description**: Untrimmed, raw RNA-seq reads in FASTQ
 format. * **Files**: 8 FASTQ files (one per sample), labeled as: *
 `AMM.fastq`: Antennal tissue, Male, Mated * `AFM.fastq`: Antennal tissue,
 Female, Mated * `AMV.fastq`: Antennal tissue, Male, Virgin * `AFV.fastq`:
 Antennal tissue, Female, Virgin * `MMM.fastq`: Maxillary palps, Male,
 Mated * `MFM.fastq`: Maxillary palps, Female, Mated * `MMV.fastq`:
 Maxillary palps, Male, Virgin * `MFV.fastq`: Maxillary palps, Female,
 Virgin #### **2. Processed Data (Trinity Assemblies)** * **Description**:
 De novo transcriptome assemblies generated using Trinity v2.13.2. Each
 assembly corresponds to one of the eight samples. * **Files**: 1
 compressed folder containing eight samples), labeled as: *
 `AMM_Trinity.fasta`, `AFM_Trinity.fasta`, ..., `MFV_Trinity.fasta` *
 **Assembly Details**: * Trinity parameters: Default settings (`--seqType
 fq --max_memory 50G`). * Quality control: Raw reads were trimmed using
 Trimmomatic v0.39 (adapters removed, minimum Phred score = 20). ###
 **Sample Naming Convention** Each sample code is a 3-letter abbreviation:
 * **First letter**: Tissue type * **A**: Antennae * **M**: Maxillary palps
 * **Second letter**: Sex * **M**: Male * **F**: Female * **Third letter**:
 Mating status * **M**: Mated * **V**: Virgin **Example**: * `AMM` =
 Antennae, Male, Mated * `MFV` = Maxillary palps, Female, Virgin ###
 **Experimental Context** * **Organism**: *Tuta absoluta* * **Tissue
 Source**: Antennae and maxillary palps dissected from adult insects. *
 **Sequencing Platform**: Illumina NovaSeq 6000 (paired-end, 150 bp reads).
 * **Biological Replicates**: Indicate if replicates are included; e.g.,
 "n = 16 per sample group". ### **Recommended Use Cases** 1.
 **Raw FASTQ Files**: For reanalysis of read alignment, differential
 expression (e.g., using tools like HISAT2, Salmon, or Kallisto), or
 quality control. 2. **Trinity Assemblies**: For transcript quantification,
 annotation (e.g., BLAST, InterProScan), or comparative analysis of
 tissue-/sex-specific gene expression. ### **Ethics and Attribution** *
 **License**: CC0 1.0 Universal (CC0 1.0). * **Citation**: If using this
 dataset, please cite both this Dryad repository and the associated
 publication: [Insert publication citation here]. ### **Contact** For
 questions, contact: * **Name**: Edson Ishengoma * **Email**:
 [edson.ishengoma@udsm.ac.tz](mailto:edson.ishengoma@udsm.ac.tz) *
 **Institution**: Mkwawa University College of Education
 **Acknowledgments**: Sequencing was supported by Mkwawa University College