cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11925567
title: Tumor-associated trypsin inhibitor (TATI) and tumor-associated trypsin-2 (TAT-2) predict outcomes in gastric cancer

description: <b>Introduction:</b> Tumor-associated trypsin inhibitor (TATI) limits serine proteases, promotes carcinogenesis in several cancers and functions as an acute-phase reactant. Tumor-associated trypsin-2 (TAT-2), a proteolytic target enzyme for TATI, can enhance invasion by promoting extracellular matrix degradation. Here, we aimed to study serum TATI and TAT-2 levels, including the TAT-2/TATI ratio, as prognostic and diagnostic biomarkers in gastric cancer. We compared the results with the plasma level of C-reactive protein (CRP). <b>Material and Methods:</b> We selected 240 individuals operated on for gastric adenocarcinoma at the Helsinki University Hospital, Finland, between 2000 and 2009. We determined the preoperative serum TAT-2, TATI and plasma CRP levels using time-resolved immunofluorometric assays using monoclonal antibodies. <b>Results:</b> The medium serum TAT-2 level was higher among gastric cancer patients [8.68 ng/ml; interquartile range (IQR) 5.93–13.2] than among benign controls (median 5.41 ng/ml; IQR 4.12–11.8; <i>p</i> = .005). Five-year survival among patients with a high serum TAT-2 was 22.9% [95% confidence interval (CI) 11.7–34.1], compared to 52.2% (95% CI 44.6–59.8; <i>p</i> p p = .037). <b>Conclusions:</b> This study shows for the first time that a high serum TAT-2 may function as a prognostic biomarker in gastric cancer and that TAT-2 levels may be elevated compared to controls. Additionally, we show that the prognosis is worse among gastric cancer patients with a high serum TATI. These biomarkers serve as prognostic factors particularly among patients with a metastatic or a locally advanced disease.
DOI: 10.5281/zenodo.5535630
title: Microbiome profile of patients in a referral gastrointestinal diseases centre in the Sudan

description: Data set of 16S rRNA Nanopore sequencing of DNA extracted from faecal samples of Gastrointestinal tract diseases from Human.
DOI: 10.5281/zenodo.5535629
title: Microbiome profile of patients in a referral gastrointestinal diseases centre in the Sudan

description: Data set of 16S rRNA Nanopore sequencing of DNA extracted from faecal samples of Gastrointestinal tract diseases from Human.
DOI: 10.6084/m9.figshare.4737142
title: Protective effects of <i>Cassia tora</i> leaves in experimental cataract by modulating intracellular communication, membrane co-transporters, energy metabolism and the ubiquitin-proteasome pathway

description: <b>Context:</b> Cataract is the clouding of eye lens which causes impairment in vision and accounts for the leading factor of global blindness. Functional food-based prevention of cataract finds application in vision research because of its availability and easy access to all classes of the society. <i>Cassia tora</i> Linn. (Caesalpinaceae) is an edible plant mentioned in the traditional systems of medicine for whole body health, especially to the eyes. <b>Objective:</b> The present study evaluates the potential of ethyl acetate fraction of <i>Cassia tora</i> leaves (ECT) on experimental cataract. <b>Materials and methods:</b> Cataract was induced by a single subcutaneous injection of sodium selenite (4 μg/g body weight) on 10th day. ECT was supplemented orally from 8th day up to 12th day at a concentration of 5 μg/g body weight and marker parameters were evaluated after 30 days. <b>Results:</b> The production of MPO and the activation of calpain were reduced 52.17% and 36.67% by ECT in lens tissue, respectively. It modulated the energy status by significantly increasing the activity of CCO 1 (55.56%) and ATP production (41.88%). ECT maintained the ionic balance in the lens by reducing the level of sodium (50%) and increasing the level of potassium (42.5%). It also reduced cell junction modifications and preserved a functional ubiquitin-proteasome pathway. <b>Discussion and conclusion:</b> The results reinforce the growing attention on wild plant food resources for preventive protection against cataract. The data suggest the value of <i>Cassia tora</i> leaves as a functional food for ameliorating cataract pathology.
DOI: 10.6084/m9.figshare.4498913
title: Silibinin sensitizes chemo-resistant breast cancer cells to chemotherapy

description: <b>Context:</b> Multiple drug resistance is the major obstacle to conventional chemotherapy. Silibinin, a nontoxic naturally occurring compound, has anticancer activity and can increase the cytotoxic effects of chemotherapy in various cancer models. <b>Objective:</b> To evaluate the effects of silibinin on enhancing the sensitivity of chemo-resistant human breast cell lines to doxorubicin (DOX) and paclitaxel (PAC). <b>Materials and methods:</b> The cells were treated with silibinin (at 50 to 600 μM concentrations) and/or chemo drugs for 24 and 48 h, then cell viability and changes in oncogenic proteins were determined by MTT assay and Western blotting/RT-PCR, respectively. Flow cytometry was used to study apoptosis in the cells receiving different treatments. The antitumorigenic effects of silibinin (at 200 to 400 μM concentration) were evaluated by mammosphere assay. <b>Results:</b> Silibinin exerted significant growth inhibitory effects with IC<sub>50</sub> ranging from 200 to 570 μM in different cell lines. Treatment of DOX-resistant MDA-MB-435 cells with silibinin at 200 μM reduced DOX IC<sub>50</sub> from 71 to 10 μg/mL and significantly suppressed the key oncogenic pathways including STAT3, AKT, and ERK in these cells. Interestingly treatment of DOX-resistant MDA-MB-435 cells with silibinin at 400 μM concentration for 48 h induced a 50% decrease in the numbers of colonies as compared with DMSO-treated cells. Treatment of PAC-resistant MCF-7 cells with silibinin at 400 μM concentration generated synergistic effects when it was used in combination with PAC at 250 nM concentration (CI = 0.81). <b>Conclusion:</b> Silibinin sensitizes chemo-resistant cells to chemotherapeutic agents and can be useful in treating breast cancers.
DOI: 10.6084/m9.figshare.4001457
title: <i>Podophyllum hexandrum</i> ameliorates endosulfan-induced genotoxicity and mutagenicity in freshwater cyprinid fish crucian carp

description: <b>Context:</b> Medicinal plants continue to act as a repository for novel drug leads with novel mechanisms of action. <i>Podophyllum hexandrum</i> Royale (Berberideceae) treats diverse conditions in folk medicine. <b>Objective:</b> The antimutagenic potential of <i>P. hexandrum</i> was evaluated against endosulfan-induced clastogenicity in a piscine model by cytogenetic endpoints. <b>Materials and methods:</b><i>Podophyllum hexandrum</i> rhizomes were subjected to successive solvent extraction. Fish were exposed to hexane, chloroform, ethyl acetate, methanol and aqueous extracts (15 mg/L each) of plant and endosulfan (0.05 mg/L) alone followed by their combination for antimutagenicity estimates. Chromosomal aberrations (CA) were made from kidney cells and micronuclei (MN) slides from peripheral blood erythrocytes at 48, 72 and 96 h. Antioxidant activity was analyzed by the DPPH assay. Phytochemical analyses were carried out using chromatographic and spectroscopic techniques. <b>Results:</b> Endosulfan induced significant (<i>p</i> R<sup>2 </sup>=<sup> </sup>.900) with antimutagenic activity, whereas EE-F4 seemed to act through a different mechanism. <b>Discussion and conclusion:</b> This study confirms the antimutagenic potential of the subject plant with the identification of some novel compounds, justifying their use in folk medicine, and their corresponding benefit to mankind.
DOI: 10.6084/m9.figshare.5155057.v2
title: <i>In vitro</i> and <i>in vivo</i> antidiabetic potential of extracts and a furostanol saponin from <i>Balanites aegyptiaca</i>

description: <b>Context:</b><i>Balanites aegyptiaca</i> Del. (Zygophyllaceae) fruits are well-known antidiabetic drug in Egyptian folk medicine. Nevertheless, its mechanism of action is still unclear. <b>Objectives:</b> Searching for the possible mechanisms of action of the plant and identification of its bioactive compounds. <b>Materials and methods:</b> A bio-guided protocol based on the evaluation of α‐glucosidase (AG) and aldose reductase (AR) inhibitory activities was adopted to isolate the biologically active compounds from the methanol extract (MeEx). An <i>in vivo</i> antidiabetic study was conducted for the active extract, fraction and compound using streptozotocin-induced diabetic male albino Wistar rats at two dose levels (100 and 200 mg/kg.b/wt) for 2 weeks. <b>Results:</b> Three compounds were isolated and identified: a sterol, (<b>1</b>) stigmasterol-3-<i>O</i>-β-d-glucopyranoside; a pregnane glucoside, (<b>2</b>) pregn-5-ene-3β,16β,20(R)-trio1-3-<i>O</i>-β-d-glucopyranoside; a furostanol saponin, (<b>3</b>) 26-(<i>O</i>-β-d-glucopyranosyl)-22-<i>O</i>-methylfurost-5-ene-3β,26-diol-3-<i>O</i>-β-d-glucopyranosyl-(1 → 4)-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranoside. Only compound <b>3</b> possessed significant AG and AR inhibitory activities (IC<sub>50</sub> = 3.12 ± 0.17 and 1.04 ± 0.02 μg/mL, respectively), while compounds <b>1</b> and <b>2</b> were inactive. The <i>in vivo</i> antidiabetic study revealed that MeEx and furostanol saponin <b>3</b> possessed significant activities at a dose of 200 mg/kg through reducing the fasting plasma glucose level by 46.14% and 51.39%, respectively, as well as reducing the total cholesterol by 24.44% and 31.90%, respectively. Compound <b>3</b> also caused increment in insulin and C-peptide levels by 63.56% and 65%, respectively. <b>Discussion and conclusions:</b> We presented a scientific base for using <i>Balanites aegyptiaca</i>, and shed the light on one of its saponins, as an antidiabetic agent in fasting and postprandial hyperglycaemia along with the improvement of diabetic complications.
DOI: 10.6084/m9.figshare.5155057
title: <i>In vitro</i> and <i>in vivo</i> antidiabetic potential of extracts and a furostanol saponin from <i>Balanites aegyptiaca</i>

description: <b>Context:</b><i>Balanites aegyptiaca</i> Del. (Zygophyllaceae) fruits are well-known antidiabetic drug in Egyptian folk medicine. Nevertheless, its mechanism of action is still unclear. <b>Objectives:</b> Searching for the possible mechanisms of action of the plant and identification of its bioactive compounds. <b>Materials and methods:</b> A bio-guided protocol based on the evaluation of α‐glucosidase (AG) and aldose reductase (AR) inhibitory activities was adopted to isolate the biologically active compounds from the methanol extract (MeEx). An <i>in vivo</i> antidiabetic study was conducted for the active extract, fraction and compound using streptozotocin-induced diabetic male albino Wistar rats at two dose levels (100 and 200 mg/kg.b/wt) for 2 weeks. <b>Results:</b> Three compounds were isolated and identified: a sterol, (<b>1</b>) stigmasterol-3-<i>O</i>-β-d-glucopyranoside; a pregnane glucoside, (<b>2</b>) pregn-5-ene-3β,16β,20(R)-trio1-3-<i>O</i>-β-d-glucopyranoside; a furostanol saponin, (<b>3</b>) 26-(<i>O</i>-β-d-glucopyranosyl)-22-<i>O</i>-methylfurost-5-ene-3β,26-diol-3-<i>O</i>-β-d-glucopyranosyl-(1 → 4)-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranoside. Only compound <b>3</b> possessed significant AG and AR inhibitory activities (IC<sub>50</sub> = 3.12 ± 0.17 and 1.04 ± 0.02 μg/mL, respectively), while compounds <b>1</b> and <b>2</b> were inactive. The <i>in vivo</i> antidiabetic study revealed that MeEx and furostanol saponin <b>3</b> possessed significant activities at a dose of 200 mg/kg through reducing the fasting plasma glucose level by 46.14% and 51.39%, respectively, as well as reducing the total cholesterol by 24.44% and 31.90%, respectively. Compound <b>3</b> also caused increment in insulin and C-peptide levels by 63.56% and 65%, respectively. <b>Discussion and conclusions:</b> We presented a scientific base for using <i>Balanites aegyptiaca</i>, and shed the light on one of its saponins, as an antidiabetic agent in fasting and postprandial hyperglycaemia along with the improvement of diabetic complications.
DOI: 10.6084/m9.figshare.4569553
title: Antibacterial activities of chemical constituents from the aerial parts of <i>Hedyotis pilulifera</i>

description: <b>Context:</b><i>Hedyotis pilulifera</i> (Pit.) T.N. Ninh (Rubiaceae) has been used in Vietnamese ethnomedicine; the methanol extract exhibited antibacterial activity in our preliminary screening. <b>Objectives:</b> In this study, compounds from <i>H. pilulifera</i> were isolated and their antibacterial activity <i>in vitro</i> was evaluated. <b>Materials and methods:</b> The aerial parts of <i>H. pilulifera</i> (1.4 kg) were extracted with MeOH, suspended in water and ethyl acetate extract was chromatographed on a silica gel column. The structures of isolated compounds were elucidated by the combination analyses of spectroscopy including 1D-, 2D-NMR, HRMS and in comparison with the reported NMR data in the literature. All isolated compounds were evaluated for inhibitory effect using the microdilution method toward <i>Staphylococcus aureus, Bacillus subtilis</i> and <i>Mycobacterium smegmatis,</i> and MIC values were determined. <b>Results:</b> Twenty compounds were isolated, including five triterpenoids, two steroids, two aromatic compounds, three fatty acids, one quinone derivative, one lignan glycoside, one ceramide and five glycolipids. Among these, oleanolic acid showed significant antibacterial activity against <i>M. smegmatis</i> with the MIC value of 2.5 μg/mL. Remarkably, rotungenic acid showed strong activity against <i>S. aureus, B. subtilis, M. smegmatis</i> with MIC values of 2.5, 2.5 and 1.25 μg/mL, respectively. Rotundic acid exhibited significant antibacterial activity against <i>B. subtilis</i> with the MIC value of 5 μg/mL. To the best of our knowledge, the antibacterial activity of rotungenic acid, stigmast-4-ene-3,6-dione and (2<i>S</i>,3<i>S</i>,4<i>R</i>,2′<i>R</i>)-2-(2′-hydroxytetracosanoylamino) octadecane-1,3,4-triol was reported for the first time. <b>Conclusions:</b> Oleanolic acid, rotungenic acid, and rotundic acid were considered to be useful for developing new antimicrobial therapeutic agents for human.
DOI: 10.6084/m9.figshare.4569553.v2
title: Antibacterial activities of chemical constituents from the aerial parts of <i>Hedyotis pilulifera</i>

description: <b>Context:</b><i>Hedyotis pilulifera</i> (Pit.) T.N. Ninh (Rubiaceae) has been used in Vietnamese ethnomedicine; the methanol extract exhibited antibacterial activity in our preliminary screening. <b>Objectives:</b> In this study, compounds from <i>H. pilulifera</i> were isolated and their antibacterial activity <i>in vitro</i> was evaluated. <b>Materials and methods:</b> The aerial parts of <i>H. pilulifera</i> (1.4 kg) were extracted with MeOH, suspended in water and ethyl acetate extract was chromatographed on a silica gel column. The structures of isolated compounds were elucidated by the combination analyses of spectroscopy including 1D-, 2D-NMR, HRMS and in comparison with the reported NMR data in the literature. All isolated compounds were evaluated for inhibitory effect using the microdilution method toward <i>Staphylococcus aureus, Bacillus subtilis</i> and <i>Mycobacterium smegmatis,</i> and MIC values were determined. <b>Results:</b> Twenty compounds were isolated, including five triterpenoids, two steroids, two aromatic compounds, three fatty acids, one quinone derivative, one lignan glycoside, one ceramide and five glycolipids. Among these, oleanolic acid showed significant antibacterial activity against <i>M. smegmatis</i> with the MIC value of 2.5 μg/mL. Remarkably, rotungenic acid showed strong activity against <i>S. aureus, B. subtilis, M. smegmatis</i> with MIC values of 2.5, 2.5 and 1.25 μg/mL, respectively. Rotundic acid exhibited significant antibacterial activity against <i>B. subtilis</i> with the MIC value of 5 μg/mL. To the best of our knowledge, the antibacterial activity of rotungenic acid, stigmast-4-ene-3,6-dione and (2<i>S</i>,3<i>S</i>,4<i>R</i>,2′<i>R</i>)-2-(2′-hydroxytetracosanoylamino) octadecane-1,3,4-triol was reported for the first time. <b>Conclusions:</b> Oleanolic acid, rotungenic acid, and rotundic acid were considered to be useful for developing new antimicrobial therapeutic agents for human.
DOI: 10.6084/m9.figshare.5120851
title: Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of <i>Carpolobia lutea</i>

description: <b>Context:</b> There is an unmet need to discover new treatments for Alzheimer’s disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of <i>Carpolobia lutea</i> G. Don (Polygalaceae). <b>Objective:</b> The objective of this study is to quantify <i>C. lutea</i> anti-AChE, DPPH free radical scavenging, and antioxidant activities and cell cytotoxicity. <b>Materials and methods:</b> Plant stem, leaves and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02–200 μg/mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid contents were determined, and cytotoxicity evaluated using human hepatocytes. <b>Results:</b><i>Carpolobia lutea</i> exhibited concentration-dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC<sub>50</sub> = 140 μg/mL); for the leaves, the chloroform leaf fraction (IC<sub>50</sub> = 60 μg/mL); and for roots, the methanol, ethyl acetate and aqueous root fractions (IC<sub>50</sub> = 0.3–3 μg/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf or root concentration. Total phenolic contents were the highest in the stem: ∼632 mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was the highest in the leaves: ∼297 mg quercetin equivalents/g for a chloroform leaf fraction. At 1 μg/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes. <b>Discussion and conclusions:</b><i>Carpolobia lutea</i> possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer’s and other diseases characterized by a cholinergic deficit.
DOI: 10.6084/m9.figshare.3806598
title: Fruit vinegars attenuate cardiac injury via anti-inflammatory and anti-adiposity actions in high-fat diet-induced obese rats

description: <b>Context:</b> Fruit vinegars (FVs) are used in Mediterranean folk medicine for their hypolipidemic and weight-reducing properties. <b>Objective:</b> To investigate the preventive effects of three types of FV, commonly available in Algeria, namely prickly pear [<i>Opuntia ficus-indica</i> (L.) Mill (Cectaceae)], pomegranate [<i>Punica granatum</i> L. (Punicaceae)], and apple [<i>Malus domestica</i> Borkh. (Rosaceae)], against obesity-induced cardiomyopathy and its underlying mechanisms. <b>Materials and methods:</b> Seventy-two male Wistar rats were equally divided into 12 groups. The first group served as normal control (distilled water, 7 mL/kg bw), and the remaining groups were respectively treated with distilled water (7 mL/kg bw), acetic acid (0.5% w/v, 7 mL/kg bw) and vinegars of pomegranate, apple or prickly pear (at doses of 3.5, 7 and 14 mL/kg bw, acetic acid content as mentioned above) along with a high-fat diet (HFD). The effects of the oral administration of FV for 18 weeks on the body and visceral adipose tissue (VAT) weights, plasma inflammatory and cardiac enzymes biomarkers, and in heart tissue were evaluated. <b>Results:</b> Vinegars treatments significantly (<i>p</i> <b>Discussion and conclusion:</b> These findings suggest that pomegranate, apple and prickly pear vinegars may prevent HFD-induced obesity and obesity-related cardiac complications, and that this prevention may result from the potent anti-inflammatory and anti-adiposity properties of these vinegars.
DOI: 10.15468/dl.hv7m7e
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ornithogalum hyrcanum Grossh."
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0063160-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.10897990
title: Ecological, flowering phenology, morphological and seed production of three sympatric dioecious Chamaedorea palms from Costa Rica

description: The data in the file was used to estimate the factors shaping seed production in three sympatric dioecious Chamaedorea palms in Costa Rica during the 2011-2012 season. The file contains the following fields:



Species. The name of the species: C. costaricana, C. macrospadix and C. tepejilote

ID. Identifier for each studied individual female plant.

infl. Identifier for each sampled inflorescence from each sampled female.

census.date: flowering date of each inflorescence.

days.since.oct14: number of days since the first Chamaedorea inflorescence flowered.

days.since.1st.flr: number of days since the first Chamaedorea inflorescence of each species flowered.

sync.costa: flowering overlap with C. costaricana males.

sync.macro: flowering overlap with C. macrospadix males.

sync.tepe: flowering overlap with C. tepejilote males.

neartest.female: distance to the nearest synchronously flowering conspecific female.

male.5m: number of synchronously flowering conspecific male individuals in a 5m radius

male.10m: number of synchronously flowering conspecific male individuals in a 10m radius

female.5m.edco: number of synchronously flowering conspecific female individuals in a 5m radius, after applying Ripley's (1977) edge correction.

female.10m.edco: number of synchronously flowering conspecific female individuals in a 10m radius, after applying Ripley's (1977) edge correction.

male.5m.edco: number of synchronously flowering conspecific  male individuals in a 5m radius, after applying Ripley's (1977) edge correction.

male.10m.edco: number of synchronously flowering conspecific male individuals in a 10m radius, after applying Ripley's (1977) edge correction.

no.stems: specific for C. costaricana, number of stems per individual.

height: height of the flowering stem in cm.

leaves: number of leaves of the flowering stem

leaflets: number of leaflets of the youngest leaf of the flowering stem

leaf.rachis: length in cm of the youngest leaf of the flowering stem

floral.rachis: length in cm of the inflorescence's rachis

peduncle: length in cm of the inflorescence's peduncle

no.spikes: number of spikes of the inflorescence

no.flowers: number of flowers per inflorescence

no.fruits: number of single-seeded fruits per inflorescence