cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.zbzr5a
title: Occurrence Download

description: A dataset containing 3165 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sapindus mukorossi"
 ]
}

 The dataset includes 3165 records from 150 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002167-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/90xag-1z734
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2012-12-05</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 73.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett,WM3433 E. coli minicell from Jun Liu & William Margolin</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 30 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-11/rawdata/</p>Invasion_complex_4_001.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-11/rawdata/Invasion_complex_4_001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Invasion_complex_4_001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-11/3dimage_24191/Invasion_complex_4_001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Invasion_complex_4_001.rec,  Reconstruction (Pixel Size 2.0 nm) 1k manual reconstruction, 265.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-11/3dimage_24249/Invasion_complex_4_001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ud5cng
title: Occurrence Download

description: A dataset containing 1976 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster horizontalis",
 "Country is Germany",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1976 records from 28 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002169-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/s28rw-b5d37
title: Caulobacter crescentus

description: Yves Brun strain 2837: CB15-del-hfsB. 1 of 1, holdfast mutants from Yves Brun, had to convert data type with Priism, good reconstruction; see Mar 22 DPD notebook entry. stalked cell; possible comet-tail filaments; coated-looking storage granules; triscuit-shaped dense feature midway along long axis; possible ftsZ
description: <strong>Tilt Series Date:</strong> 2005-10-05</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.5°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft, Priism</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> PYE media (RB Jensen protocol);  5ml cultures in 14ml tubes; exponential phase; see DPD notebook Sep 28-30, 2005</p>
<strong>Sample Preparation:</strong> box 641, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -1, blot 1 sec, drain 1 sec; see DPD notebook Sep 30, 2005</p>
  pdd2005-10-05-1_slicer5337.jpg: Filament .  S-layer pdd2005-10-05-1_slicer5338.jpg: Filament pdd2005-10-05-1_slicer5340.jpg: Peptidoglycan pdd2005-10-05-1_slicer5341.jpg: Filament pdd2005-10-05-1_slicer5343.jpg: Unknown pdd2005-10-05-1_slicer5348.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-05-1/rawdata/</p>YB2837_05Oct05t01.mrc,  Tilt Series (Pixel Size 1.2 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-05-1/rawdata/YB2837_05Oct05t01.mrc"
        > <i class="download icon"></i> Download </a></p>

        05Oct05t01bn.rec,  Reconstruction (Pixel Size 2.4 nm), 463.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-05-1/3dimage_259/05Oct05t01bn.rec"
        > <i class="download icon"></i> Download </a></p>

        05Oct05t01bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 786.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-05-1/3dimage_260/05Oct05t01bn_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.qgrewv
title: Occurrence Download

description: A dataset containing 111 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster horizontalis",
 "Country is Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 111 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002170-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6qahm5
title: Occurrence Download

description: A dataset containing 280 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster horizontalis",
 "Country is Italy",
 "HasCoordinate is true"
 ]
}

 The dataset includes 280 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002171-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/kcnrg-my086
title: Caulobacter crescentus

description: Yves Brun strain 2845: CB15-del-hfsD. 3 of 5, holdfast mutants from Yves Brun, had to convert data type with Priism, very clear reconstruction. very, very clear stalk with two septae; faint comet-tail filaments in short segments; super-clear S-layer on stalk
description: <strong>Tilt Series Date:</strong> 2005-10-04</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.5°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft, Priism</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> PYE media (RB Jensen protocol);  5ml cultures in 14ml tubes; exponential phase; see DPD notebook Sep 28-30, 2005</p>
<strong>Sample Preparation:</strong> box 642, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -1, blot 1 sec, drain 1 sec; see DPD notebook Sep 30, 2005</p>
  pdd2005-10-04-3_slicer5320.jpg: Septae pdd2005-10-04-3_slicer5322.jpg: Unknown pdd2005-10-04-3_slicer5323.jpg: Filament .  S-layer pdd2005-10-04-3_slicer5326.jpg: Peptidoglycan pdd2005-10-04-3_slicer5327.jpg: Filament pdd2005-10-04-3_slicer5329.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-3/rawdata/</p>YB2845_05Oct04t03.mrc,  Tilt Series (Pixel Size 1.2 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-3/rawdata/YB2845_05Oct04t03.mrc"
        > <i class="download icon"></i> Download </a></p>

        05Oct04t03.rec,  Reconstruction (Pixel Size 2.4 nm), 441.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-3/3dimage_250/05Oct04t03.rec"
        > <i class="download icon"></i> Download </a></p>

        05Oct04t03_full.rec,  Reconstruction (Pixel Size 2.4 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-3/3dimage_251/05Oct04t03_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wj8rv-wme24
title: MS411-R1-16 / WM1652

description: to capture F-piligrid box: YWC095
description: <strong>Tilt Series Date:</strong> 2014-04-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strains from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MS411-R1-16 & WM1652 grew to OD 0.6 in LB. Mix 2.5 ul of each onto grids on LB-agar plate, incubate 30 min for conjugation.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 22.5 C, 3µl BSA treated 10 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 1 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-04-07-9_slicer6980.jpg: filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-9/rawdata/</p>20140407_YWC095_20008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-9/rawdata/20140407_YWC095_20008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140407_YWC095_20008_part131_3.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-9/3dimage_41137/20140407_YWC095_20008_part131_3.rec"
        > <i class="download icon"></i> Download </a></p>

        20140407_YWC095_20008_DB.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-9/3dimage_41300/20140407_YWC095_20008_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/trk2n-crd64
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2012-12-05</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 73.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett,WM3433 E. coli minicell from Jun Liu & William Margolin</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 30 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2012-12-05-10_slicer26434.jpg: skreida. Rosehips at the pilated pole of Bdellovibrio
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-10/rawdata/</p>Invasion_complex_2_001.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-10/rawdata/Invasion_complex_2_001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Invasion_complex_2_001_part122_2.rec,  Reconstruction (Pixel Size 2.0 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-10/3dimage_24189/Invasion_complex_2_001_part122_2.rec"
        > <i class="download icon"></i> Download </a></p>

        Invasion_complex_2_merge_1_0-60_2_61-121.rec,  Reconstruction (Pixel Size 2.0 nm) 1k manual reconstruction, 265.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-12-05-10/3dimage_24250/Invasion_complex_2_merge_1_0-60_2_61-121.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c05c5-qef22
title: E. coli CusCFBA-CusC-K98H

description: Overexpressed CusCFBA efflux pump in E. coli C43 (DE3).Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-06-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Edward Yu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-24/rawdata/</p>CusCFBA_CusCK98H_20021.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-24/rawdata/CusCFBA_CusCK98H_20021.mrc"
        > <i class="download icon"></i> Download </a></p>

        CusCFBA_CusCK98H_20021_part121_8.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-24/3dimage_27266/CusCFBA_CusCK98H_20021_part121_8.rec"
        > <i class="download icon"></i> Download </a></p>

        ycw2013-06-07-24_slicer17055.png,  680.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2013-06-07-24_slicer17055.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nmczk-s6157
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter lysed n the presence of the crowding agent 10% Polyvinylpyrrolidone (PVP)
description: <strong>Tilt Series Date:</strong> 2013-10-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Protocol:-     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xg-     resuspended PVP in 80µL appropriate Buffer 2Buffer 1:Crowding agent1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0Buffer 2:Crowding agentQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-28/rawdata/</p>Rhodopvp30002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-28/rawdata/Rhodopvp30002.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp30002SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-28/3dimage_46416/Rhodopvp30002SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp30002SIRTrot.rec~,  5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-28/file_10486/Rhodopvp30002SIRTrot.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mv4mp-bhv98
title: H. pylori 26695 / Hs.746T

description: Grid box YWC057
description: <strong>Tilt Series Date:</strong> 2013-12-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
  ycw2013-12-16-28_slicer9839.jpg: fdilament bundle, Helicobacter pylori
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-28/rawdata/</p>20131216_YWC057_10022.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-28/rawdata/20131216_YWC057_10022.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131216_YWC057_10022_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-28/3dimage_36162/20131216_YWC057_10022_full.rec"
        > <i class="download icon"></i> Download </a></p>

        ycw2013-12-16-28_slicer19224.png,  REZ .  -andrew, 736.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2013-12-16-28_slicer19224.png"
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