DOI: 10.58132/ppvkn8
title: Enhancement of temozolomide stability and anticancer efficacy by loading in monopalmitolein-based cubic phase nanoparticles

description: The manuscript "Enhancement of Temozolomide Stability and Anticancer Efficacy by Loading in Monopalmitolein-Based Cubic Phase Nanoparticles" contains data from this dataset.In this study, temozolomide was incorporated into both monoolein (MO)- and monopalmitolein (MP)-derived cubic phases. Determination of the profile and kinetics of drug release was carried out. We concluded that likely the observed advantages in the release profile of MP might be related to the observed differences in the structural parameters of both systems, where the aqueous channel diameter is larger for the MP-derived formulation. Moreover, the biological studies revealed that the TMZ-loaded MP formulation presents stronger anticancer properties than the drug-loaded MO phase. What is crucial, the improved effectiveness of MP/TMZ was also observed during treatment of drug-resistant glioma-derived cells. As MP-based formulations present increased TMZ stability and high drug effectiveness, the MP/TMZ cubosomes may offer a new strategy to overcome the restrictions related to the blood-brain barrier.DATA & FILE OVERVIEWFile List: Scheme 1. Chemical structures of the used reagentsFig1A.csv Diffractograms of cubic phases prepared from monoolein (MO) and acetate buffer, doped with various concentrations of temozolomide (TMZ); Fig1B.csv Diffractograms for cubic phases prepared from monopalmitolein (MP) and temozolomide;Fig1C.csv Diffractograms of cubosomes prepared from MO or MP and acetate buffer, doped with TMZ. Fig2A.csv Cyclic voltammogram recorded on the glassy carbon electrode (GCE) for TMZ in an acetic buffer (pH of 5.0); scan rate 50 mV s–1; Fig2B.csv Differential pulse voltammograms recorded on the GCE for the MO and MP cubic phases; amplitude: ?E = 50 mV, pulse time: tp = 50 ms; Fig2C.csv Drug release profile for TMZ-loaded MO and MP cubic phases; D) TMZ release profile from cubic phase nanoparticles.Fig3.csv Ratio of the relaxation input (R) and the diffusion input according to Fick (F) (R/F ratio) for TMZ release from the MO- and MP-based A) cubic phase and B) cubosomes.Fig4A.csv Analysis of the survival rate of A-172 and T98G cells treated with free TMZ (TMZ) and TMZ-loaded MO or MP phases (MO/TMZ; MP/TMZ, respectively). The cell’s viability was measured using MTS assay. Data are presented as mean ± SD (standard deviation); (n = 8). Non-treated cells served as controls. * p &lt; 0.05.Fig4B.csv Analysis of the survival rate of A-172 and T98G cells treated with free TMZ (TMZ) and TMZ-loaded MO or MP phases (MO/TMZ; MP/TMZ, respectively). The cell’s viability was measured using trypan blue exclusion assay. Data are presented as mean ± SD (standard deviation); (n = 8). Non-treated cells served as controls. * p &lt; 0.05.Fig5.csv Flow cytometry analysis of A-172 and T98G cells treated with free TMZ and TMZ-loaded MO or MP phases. The light color bars show the number of viable cells (V), while the dark color bars refer to the number of apoptotic cells (A) in each of the tested samples. Data are presented as mean ± SD; (n = 10). Control?non-treated cells. * p &lt; 0.05.Fig6.tiff Click-iT TUNEL Alexa Fluor 647 Imaging Assay of A-172 cells exposed to free TMZ, TMZ-loaded MO or TMZ-loaded MP phases for 24 h (representative images). Non-treated cells or cells treated with non-loaded MO or non-loaded MP particles served as controls. The intensity of the red signal (Alexa Fluor 647) refers to the apoptotic rate in the tested cells. The green signal (phalloidin-FITC) indicates the organization of the cell structure. Cell nuclei were counterstained with DAPI (blue signal). Objective: 63x/1.4 oil DIC M27. Panel A – standard confocal imaging; Panel B – Airyscan imaging.METHODOLOGICAL INFORMATIONDescription of methods used for collection/generation of data: small angle X-ray scattering, voltammetry.Instrument- or software-specific information needed to interpret the data: csv data can be opened with e.g. Excel, Origin, jpg (tiff) files contain images.