cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19920607
title: Temporo-spatial distribution of stem cell markers CD146 and p75NTR during odontogenesis in mice

description: Abstract Mesenchymal and epithelial stem cells were identified in dental tissues; however, knowledge about the odontogenic stem cells is limited, and there are some questions regarding their temporo-spatial dynamics in tooth development. Objective Our study aimed to analyze the expression of the stem cell markers CD146 and p75NTR during the different stages of odontogenesis. Methodology The groups consisted of 13.5, 15.5, 17.5 days old embryos, and 14 days postnatal BALB/c mice. The expression of CD146 and p75NTR was evaluated by immunohistochemistry. Results Our results showed that positive cells for both markers were present in all stages of tooth development, and the number of positive cells increased with the progression of this process. Cells of epithelial and ectomesenchymal origin were positive for CD146, and the expression of p75NTR was mainly detected in the dental papilla and dental follicle. In the postnatal group, dental pulp cells were positive for CD146, and the reduced enamel epithelium and the oral mucosa epithelium showed immunostaining for p75NTR. Conclusions These results suggest that the staining pattern of CD146 and p75NTR underwent temporal and spatial changes during odontogenesis and both markers were expressed by epithelial and mesenchymal cell types, which is relevant due to the significance of the epithelial-ectomesenchymal interactions in tooth development.
DOI: 10.6084/m9.figshare.19920607.v1
title: Temporo-spatial distribution of stem cell markers CD146 and p75NTR during odontogenesis in mice

description: Abstract Mesenchymal and epithelial stem cells were identified in dental tissues; however, knowledge about the odontogenic stem cells is limited, and there are some questions regarding their temporo-spatial dynamics in tooth development. Objective Our study aimed to analyze the expression of the stem cell markers CD146 and p75NTR during the different stages of odontogenesis. Methodology The groups consisted of 13.5, 15.5, 17.5 days old embryos, and 14 days postnatal BALB/c mice. The expression of CD146 and p75NTR was evaluated by immunohistochemistry. Results Our results showed that positive cells for both markers were present in all stages of tooth development, and the number of positive cells increased with the progression of this process. Cells of epithelial and ectomesenchymal origin were positive for CD146, and the expression of p75NTR was mainly detected in the dental papilla and dental follicle. In the postnatal group, dental pulp cells were positive for CD146, and the reduced enamel epithelium and the oral mucosa epithelium showed immunostaining for p75NTR. Conclusions These results suggest that the staining pattern of CD146 and p75NTR underwent temporal and spatial changes during odontogenesis and both markers were expressed by epithelial and mesenchymal cell types, which is relevant due to the significance of the epithelial-ectomesenchymal interactions in tooth development.
DOI: 10.6084/m9.figshare.19920859
title: Soil biological attributes in monoculture and integrated systems in the Cerrado region of Piauí State, Brazil

description: ABSTRACT The implementation of integrated agricultural production systems is considered a promising strategy for sustainable agricultural intensification in Brazil. This study aimed to evaluate the effects of different monoculture and integrated production systems on the microbiological attributes and organic carbon of soil from the Cerrado region in Piauí, Brazil. Soil samples were collected from the 0.0-0.10-m layer in the following systems: no-tillage (PD), pasture (PAS), exclusive eucalyptus cultivation (CEE), integrated livestock-forest system (IPF) and a native Cerrado (CN) area, which was used as reference. Total organic carbon (TOC) and nitrogen (NT) contents, microbial biomass carbon (CMIC), microbial respiration (MR), microbial quotient (qMIC), metabolic quotient (qCO2), as well as the activities of the hydrolysis of fluorescein diacetate (FDA), acid phosphatase, β-glucosidase and urease enzymes were evaluated. High TOC contents were found in the CEE, IPF and PAS systems, and high CMIC and qMIC values were found in the CN and CEE systems. The variables MR, qCO2 and enzymatic activity varied as a function of the management systems. The IPF and CEE systems caused improvements in the soil attributes, with increases in organic carbon and microbial biomass. The IPF integrated production system promoted improvements in the microbiological indicators of soil quality and was considered an environmentally sustainable agricultural production system. The transition from CN to agricultural areas caused changes in the soil microbiological indicators, which were perceived several years after anthropogenic intervention, indicating that even with the adoption of conservation systems, it was not possible to reestablish the soil microbial biomass.
DOI: 10.6084/m9.figshare.19920859.v1
title: Soil biological attributes in monoculture and integrated systems in the Cerrado region of Piauí State, Brazil

description: ABSTRACT The implementation of integrated agricultural production systems is considered a promising strategy for sustainable agricultural intensification in Brazil. This study aimed to evaluate the effects of different monoculture and integrated production systems on the microbiological attributes and organic carbon of soil from the Cerrado region in Piauí, Brazil. Soil samples were collected from the 0.0-0.10-m layer in the following systems: no-tillage (PD), pasture (PAS), exclusive eucalyptus cultivation (CEE), integrated livestock-forest system (IPF) and a native Cerrado (CN) area, which was used as reference. Total organic carbon (TOC) and nitrogen (NT) contents, microbial biomass carbon (CMIC), microbial respiration (MR), microbial quotient (qMIC), metabolic quotient (qCO2), as well as the activities of the hydrolysis of fluorescein diacetate (FDA), acid phosphatase, β-glucosidase and urease enzymes were evaluated. High TOC contents were found in the CEE, IPF and PAS systems, and high CMIC and qMIC values were found in the CN and CEE systems. The variables MR, qCO2 and enzymatic activity varied as a function of the management systems. The IPF and CEE systems caused improvements in the soil attributes, with increases in organic carbon and microbial biomass. The IPF integrated production system promoted improvements in the microbiological indicators of soil quality and was considered an environmentally sustainable agricultural production system. The transition from CN to agricultural areas caused changes in the soil microbiological indicators, which were perceived several years after anthropogenic intervention, indicating that even with the adoption of conservation systems, it was not possible to reestablish the soil microbial biomass.
DOI: 10.6084/m9.figshare.19920861.v1
title: Prediction of Italian ryegrass (Lolium multiflorum L.) emergence using soil thermal time

description: ABSTRACT Italian ryegrass (Lolium multiflorum L.) is a highly competitive weed widely disseminated worldwide that affects both summer and winter crops. The development of predictive emergence models can contribute to optimizing weed management. The aim of this study was to develop and validate an empirical emergence model of Italian ryegrass based on soil thermal time. For model development, cumulative emergence in two locations was obtained, and the model was validated with data collected in an experiment conducted independently. Three commonly used emergence models were compared (Gompertz, Logistic, and Weibull). The relationship between emergence and soil thermal time was described best by the Gompertz model. The Gompertz model predicted Italian ryegrass emergence start at 300 thermal time (TT), reaching 50% emergence at 444 TT, and 90% at 590 TT. Model validation performed well in predicting Italian ryegrass emergence and proved to be efficient at describing its emergence. This is a potential predictive tool for assisting farmers with Italian ryegrass management.
DOI: 10.6084/m9.figshare.19920861
title: Prediction of Italian ryegrass (Lolium multiflorum L.) emergence using soil thermal time

description: ABSTRACT Italian ryegrass (Lolium multiflorum L.) is a highly competitive weed widely disseminated worldwide that affects both summer and winter crops. The development of predictive emergence models can contribute to optimizing weed management. The aim of this study was to develop and validate an empirical emergence model of Italian ryegrass based on soil thermal time. For model development, cumulative emergence in two locations was obtained, and the model was validated with data collected in an experiment conducted independently. Three commonly used emergence models were compared (Gompertz, Logistic, and Weibull). The relationship between emergence and soil thermal time was described best by the Gompertz model. The Gompertz model predicted Italian ryegrass emergence start at 300 thermal time (TT), reaching 50% emergence at 444 TT, and 90% at 590 TT. Model validation performed well in predicting Italian ryegrass emergence and proved to be efficient at describing its emergence. This is a potential predictive tool for assisting farmers with Italian ryegrass management.
DOI: 10.6084/m9.figshare.19920864
title: Porcine circovirus 2 and 3 in wild boars in Southern Brazil

description: ABSTRACT: Porcine circovirus 2 (PCV2) has a considerable economic impact on the pork industry worldwide for more than two decades. In 2016, a new circovirus, porcine circovirus 3 (PCV3), was described; since then, it has been reported to be associated with diseased or even in clinically healthy swine in several countries. Considering the importance of wild boars as reservoirs of swine pathogens and the extensive distribution of these animals in Rio Grande do Sul and throughout the national territory, we searched for PCV2 and PCV3 in twenty-six wild boars coupled with necropsy and histologic examination of the sampled animals. Using PCR, 182 tissue samples were analyzed, including the heart, kidneys, liver, lung, lymph nodes, spleen, and tonsils. PCV2 and PCV3 were detected in 57.7% (15/26) and 15.4% (4/26) of wild boars, respectively. Furthermore, co-infection with PCV2 and PCV3 was detected in one of these animals, with PCV2 or PCV3 DNA detection in multiple organs. Histological examination showed mild to moderate and multifocal lymphoplasmacytic interstitial nephritis distributed randomly throughout the renal cortex, apparently unrelated to PCV2 or PCV3 detection. The wild boar population in Brazil is extensive, indicating the presence of a larger number of swine pathogen hosts. In the present study, more than half of the wild boars harbored PCV2; and although less frequently, PCV3 was also detected. Therefore, free-living wild boars can serve as reservoirs of swine circoviruses in southern Brazil.
DOI: 10.6084/m9.figshare.19920864.v1
title: Porcine circovirus 2 and 3 in wild boars in Southern Brazil

description: ABSTRACT: Porcine circovirus 2 (PCV2) has a considerable economic impact on the pork industry worldwide for more than two decades. In 2016, a new circovirus, porcine circovirus 3 (PCV3), was described; since then, it has been reported to be associated with diseased or even in clinically healthy swine in several countries. Considering the importance of wild boars as reservoirs of swine pathogens and the extensive distribution of these animals in Rio Grande do Sul and throughout the national territory, we searched for PCV2 and PCV3 in twenty-six wild boars coupled with necropsy and histologic examination of the sampled animals. Using PCR, 182 tissue samples were analyzed, including the heart, kidneys, liver, lung, lymph nodes, spleen, and tonsils. PCV2 and PCV3 were detected in 57.7% (15/26) and 15.4% (4/26) of wild boars, respectively. Furthermore, co-infection with PCV2 and PCV3 was detected in one of these animals, with PCV2 or PCV3 DNA detection in multiple organs. Histological examination showed mild to moderate and multifocal lymphoplasmacytic interstitial nephritis distributed randomly throughout the renal cortex, apparently unrelated to PCV2 or PCV3 detection. The wild boar population in Brazil is extensive, indicating the presence of a larger number of swine pathogen hosts. In the present study, more than half of the wild boars harbored PCV2; and although less frequently, PCV3 was also detected. Therefore, free-living wild boars can serve as reservoirs of swine circoviruses in southern Brazil.
DOI: 10.6084/m9.figshare.19920865
title: Milking practices on commercial Holstein-Friesian farms

description: ABSTRACT The objective of this study was to survey and analyze milking practices of commercial Holstein-Friesian farms. A total of 43 Hungarian dairy farms with 31,430 cows was surveyed by using a questionnaire via personal interviews. Furthermore, seven in-depth, individual interviews were conducted with farm managers. In the statistical analysis, we used ANOVA models. The results showed that 57.8% of the milking parlors had a herringbone design, followed by parallel (20.0%) and rotary (17.8%) milking systems. For the interviewed farm managers, gentle (71.4%) and quick milking (57.1%) as well as herd size (57.1%) were the most important factors in determining the milking system design. In 62.8% of the farms, cows were milked twice a day, and the average milking time was 5.0 h with an average of 3.1 laborers. The average daily milking time per cow was 15 min and the average daily walking time per cow to the milking parlor and back was 24 min. Furthermore, 85.4% of the farms used traditional elastic milk liners, whereas 14.6% used silicone ones. In total, 57.1% of the interviewed farm managers said that the ideal teat liner should fit the teats correctly and provide gentle milking. Prior to milking, 65.1% of the farms used disinfectant dip and 11.6% used a disinfectant wash, whereas 23.3% still washed the udder with water. The udder was wiped with paper towels in 73.8% of the herds and with cloth in 26.2% of the herds. Forestripping was performed in all herds: 51.2% onto the floor, 46.5% into a cup, and in 2.3% into a paper towel. Further, 85.7% of the interviewed farm managers considered the use of a cup to be the ideal method, but 57.1% deemed a dark-colored piece of rubber/flooring to be similarly acceptable. Cows with mastitis were milked separately in 91.9% of the farms.
DOI: 10.6084/m9.figshare.19920865.v1
title: Milking practices on commercial Holstein-Friesian farms

description: ABSTRACT The objective of this study was to survey and analyze milking practices of commercial Holstein-Friesian farms. A total of 43 Hungarian dairy farms with 31,430 cows was surveyed by using a questionnaire via personal interviews. Furthermore, seven in-depth, individual interviews were conducted with farm managers. In the statistical analysis, we used ANOVA models. The results showed that 57.8% of the milking parlors had a herringbone design, followed by parallel (20.0%) and rotary (17.8%) milking systems. For the interviewed farm managers, gentle (71.4%) and quick milking (57.1%) as well as herd size (57.1%) were the most important factors in determining the milking system design. In 62.8% of the farms, cows were milked twice a day, and the average milking time was 5.0 h with an average of 3.1 laborers. The average daily milking time per cow was 15 min and the average daily walking time per cow to the milking parlor and back was 24 min. Furthermore, 85.4% of the farms used traditional elastic milk liners, whereas 14.6% used silicone ones. In total, 57.1% of the interviewed farm managers said that the ideal teat liner should fit the teats correctly and provide gentle milking. Prior to milking, 65.1% of the farms used disinfectant dip and 11.6% used a disinfectant wash, whereas 23.3% still washed the udder with water. The udder was wiped with paper towels in 73.8% of the herds and with cloth in 26.2% of the herds. Forestripping was performed in all herds: 51.2% onto the floor, 46.5% into a cup, and in 2.3% into a paper towel. Further, 85.7% of the interviewed farm managers considered the use of a cup to be the ideal method, but 57.1% deemed a dark-colored piece of rubber/flooring to be similarly acceptable. Cows with mastitis were milked separately in 91.9% of the farms.
DOI: 10.6084/m9.figshare.19920871
title: On a trip to the mainland: occasional records of the rocky crab Grapsus grapsus (Linnaeus, 1758) (Decapoda: Grapsidae) on the Brazilian coast

description: Abstract The Sally lightfoot crab, Grapsus grapsus (Linnaeus, 1758), is distributed along the Eastern Pacific coast and along the Western Atlantic coast, from the Gulf of Mexico to the Southwestern Atlantic. In Brazil, the only established populations are found on the Brazilian oceanic islands, although two previous records (1901 and 1966) reported the presence of individuals on the continental coast. Here, we report new records of the Sally lightfoot crab on the coast of the states of Espírito Santo and Rio Grande do Norte, southeastern and northeastern Brazil, respectively. We also discuss three main hypotheses that could explain occasional records (1901, 1966, 2005, and 2020) of single individuals on the mainland: rafting events associated with flotsam and debris carried by oceanic currents; hitchhiking on vessels and fishing boats navigating routes from the Brazilian oceanic islands to the mainland; and stochastic settlement through larval dispersal.
DOI: 10.6084/m9.figshare.19920871.v1
title: On a trip to the mainland: occasional records of the rocky crab Grapsus grapsus (Linnaeus, 1758) (Decapoda: Grapsidae) on the Brazilian coast

description: Abstract The Sally lightfoot crab, Grapsus grapsus (Linnaeus, 1758), is distributed along the Eastern Pacific coast and along the Western Atlantic coast, from the Gulf of Mexico to the Southwestern Atlantic. In Brazil, the only established populations are found on the Brazilian oceanic islands, although two previous records (1901 and 1966) reported the presence of individuals on the continental coast. Here, we report new records of the Sally lightfoot crab on the coast of the states of Espírito Santo and Rio Grande do Norte, southeastern and northeastern Brazil, respectively. We also discuss three main hypotheses that could explain occasional records (1901, 1966, 2005, and 2020) of single individuals on the mainland: rafting events associated with flotsam and debris carried by oceanic currents; hitchhiking on vessels and fishing boats navigating routes from the Brazilian oceanic islands to the mainland; and stochastic settlement through larval dispersal.
DOI: 10.6084/m9.figshare.19920874
title: School ethnography on intercultural education in three Mapuche schools in La Araucanía

description: ABSTRACT Since 1996 the educational policy of the Chilean State implements a Programa de Educación Intercultural Bilingüe (PEIB) [Program of Intercultural Bilingual Education], as a form of historical reparation with indigenous peoples, incorporating learning content about the language and culture of these peoples into the school curriculum. The article presents research results on the implementation of the Educación Intercultural Bilingüe (EIB) [Bilingual Intercultural Education], in three schools in La Araucanía. The methodology is qualitative, with a descriptive level of exploratory scope. The results account for socio-pedagogical factors that limit the implementation of the EIB, with an asymmetric and epistemologically stressed educational relationship prevailing between the mentor teacher, the traditional educator, and the students. We conclude that even though the EIB has been in the Chilean school system for more than twenty years, it would not be contributing to the development of additive bilingualism, by relearning the indigenous language.
DOI: 10.6084/m9.figshare.19920874.v1
title: School ethnography on intercultural education in three Mapuche schools in La Araucanía

description: ABSTRACT Since 1996 the educational policy of the Chilean State implements a Programa de Educación Intercultural Bilingüe (PEIB) [Program of Intercultural Bilingual Education], as a form of historical reparation with indigenous peoples, incorporating learning content about the language and culture of these peoples into the school curriculum. The article presents research results on the implementation of the Educación Intercultural Bilingüe (EIB) [Bilingual Intercultural Education], in three schools in La Araucanía. The methodology is qualitative, with a descriptive level of exploratory scope. The results account for socio-pedagogical factors that limit the implementation of the EIB, with an asymmetric and epistemologically stressed educational relationship prevailing between the mentor teacher, the traditional educator, and the students. We conclude that even though the EIB has been in the Chilean school system for more than twenty years, it would not be contributing to the development of additive bilingualism, by relearning the indigenous language.
DOI: 10.6084/m9.figshare.19920876.v1
title: “In close contact with nature”: education, health and labor at the School Hospital José de Mendonça

description: ABSTRACT This study aims to examine the practices of the School Hospital José de Mendonça, founded in the late 1930s in the city Araruama, in the countryside of Rio de Janeiro. With structures and activities marked by medical and educational reasons, the school first gathered children of public Rio de Janeiro schools, who suffered from treatable or curable diseases. This institution exposed the children to formal education, labor, and physical practices guided by natural medicine - for example, sea and sunbathing, exposure to sun, and sea air were used during treatment. The goals of the school-hospital, conceived as a counterpoint to busy and harmful city life, articulated with the belief in a nature that can restore, relieve, and invigorate. The discourses of the creator, Doctor Oscar Clark, offer clues about the bigger picture in which the initiative is inscribed: a modern project, defended by fractions of the Brazilian social thought, which had as cornerstones the cure of a people considered sick, whose redemption was literacy, labor and practices of body education.
DOI: 10.6084/m9.figshare.19920876
title: “In close contact with nature”: education, health and labor at the School Hospital José de Mendonça

description: ABSTRACT This study aims to examine the practices of the School Hospital José de Mendonça, founded in the late 1930s in the city Araruama, in the countryside of Rio de Janeiro. With structures and activities marked by medical and educational reasons, the school first gathered children of public Rio de Janeiro schools, who suffered from treatable or curable diseases. This institution exposed the children to formal education, labor, and physical practices guided by natural medicine - for example, sea and sunbathing, exposure to sun, and sea air were used during treatment. The goals of the school-hospital, conceived as a counterpoint to busy and harmful city life, articulated with the belief in a nature that can restore, relieve, and invigorate. The discourses of the creator, Doctor Oscar Clark, offer clues about the bigger picture in which the initiative is inscribed: a modern project, defended by fractions of the Brazilian social thought, which had as cornerstones the cure of a people considered sick, whose redemption was literacy, labor and practices of body education.