cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8150597
title: Fig-3. Dicer suppression is associated with downregulation of AQP2, NKCC2 and lower density of collecting ducts.

description: Fig-3 shows the representative pictures of the inner stripe of the outer medulla of control (A, C) and Dicer cKO (B, D) mice double labeled with anti-AQP2 (green) and anti-H+ATPase (red) antibodies. At P50, Dicer cKO mice (D) showed no alteration in the distribution pattern of principal (AQP2 +) and intercalated cells (H+ATPase +), but a lower density of collecting ducts compared to the controls (C). (A-D) Magnification 40X. (E) Western blot analysis for AQP2 and NKCC2 expression at P30 (upper) and P50 (lower) of samples of IM and ISOM, respectively. AQP2 expression is significantly downregulated in Dicer cKO mice compared to Ctr at P50. NKCC2 expression is significantly downregulated in Dicer cKO mice compared to Ctr at P30 and P50. Data are expressed as mean ± sem; n power is 5 vs 5. *** is for p value &lt; 0.001.
DOI: 10.6084/m9.figshare.8150597.v1
title: Fig-3. Dicer suppression is associated with downregulation of AQP2, NKCC2 and lower density of collecting ducts.

description: Fig-3 shows the representative pictures of the inner stripe of the outer medulla of control (A, C) and Dicer cKO (B, D) mice double labeled with anti-AQP2 (green) and anti-H+ATPase (red) antibodies. At P50, Dicer cKO mice (D) showed no alteration in the distribution pattern of principal (AQP2 +) and intercalated cells (H+ATPase +), but a lower density of collecting ducts compared to the controls (C). (A-D) Magnification 40X. (E) Western blot analysis for AQP2 and NKCC2 expression at P30 (upper) and P50 (lower) of samples of IM and ISOM, respectively. AQP2 expression is significantly downregulated in Dicer cKO mice compared to Ctr at P50. NKCC2 expression is significantly downregulated in Dicer cKO mice compared to Ctr at P30 and P50. Data are expressed as mean ± sem; n power is 5 vs 5. *** is for p value &lt; 0.001.
DOI: 10.6084/m9.figshare.8150588
title: Fig-7. The development of the glomerulocystic phenotype is progressively associated with loss of primary cilium.

description: Fig-7 Representative pictures of renal cortex from 50 days old Ctr (B) and Dicer cKO mice (A, C, D) stained for α-Acetylated Tubulin (green) and counterstained with Dapi (blue). Primary cilium isnormally found in non-dilating tubules of Dicer cKO mice (A) and in the parietal cells of Ctr mice (B) at P50. However, presence of cilia is decreased progressively in dilating glomeruli (C) until disappearing in frank cysts (D). Bars = 5 μm (A); 10 μm (B-D).
DOI: 10.6084/m9.figshare.8150585
title: Fig-8. Alterations of GSK3β / β-catenin are associated with the development of the glomerulocystic phenotype.

description: Fig-8 Representative pictures of renal cortex from 30 (A, B) and 50 days old (C, D) mice. Ctr (A, C) and Dicer cKO (B, D) sections were stained with anti β-catenin antibody. In Dicer cKO, β-catenin expression is limited to the basolateral domain in both tubules and parietal cells of the Bowman’s capsule, at both P30 and P50 (B-D). (A-D) Magnification63X, zoom1.5. Panel E and F show immunoblotting of Cortex/OSOM samples of 30 (E) and 50 (F) days old mice. GSK3β expression is already upregulatedcat the pre-cystic stage (P30) in Dicer cKO mice (E) and this is persistent at P50 (F) n power is 5 vs 5. Glomerular cyst formation is associated with loss of cytosolic β-catenin expression and general downregulation of cortical protein level (F). No significant changes were observed in the fraction of their main regulating phosphorylated forms. Panel G represent qPCR analysis of mRNA of β-catenin in 30 (right) and 50 days old mice (left). mRNA level of β-catenin is downregulated in Dicer-cKO mice at P50. Data are expressed as mean ± sem; * is for p value &lt; 0.05; ** is for p-value &lt;0.01.
DOI: 10.6084/m9.figshare.8150585.v1
title: Fig-8. Alterations of GSK3β / β-catenin are associated with the development of the glomerulocystic phenotype.

description: Fig-8 Representative pictures of renal cortex from 30 (A, B) and 50 days old (C, D) mice. Ctr (A, C) and Dicer cKO (B, D) sections were stained with anti β-catenin antibody. In Dicer cKO, β-catenin expression is limited to the basolateral domain in both tubules and parietal cells of the Bowman’s capsule, at both P30 and P50 (B-D). (A-D) Magnification63X, zoom1.5. Panel E and F show immunoblotting of Cortex/OSOM samples of 30 (E) and 50 (F) days old mice. GSK3β expression is already upregulatedcat the pre-cystic stage (P30) in Dicer cKO mice (E) and this is persistent at P50 (F) n power is 5 vs 5. Glomerular cyst formation is associated with loss of cytosolic β-catenin expression and general downregulation of cortical protein level (F). No significant changes were observed in the fraction of their main regulating phosphorylated forms. Panel G represent qPCR analysis of mRNA of β-catenin in 30 (right) and 50 days old mice (left). mRNA level of β-catenin is downregulated in Dicer-cKO mice at P50. Data are expressed as mean ± sem; * is for p value &lt; 0.05; ** is for p-value &lt;0.01.
DOI: 10.17632/22gzxp66xt.2
title: The YdiU Domain Modulates Bacterial Stress Signaling through Mn2+-dependent UMPylation

description: Original images of a manuscript titled The YdiU Domain Modulates Bacterial Stress Signaling through Mn2+-dependent UMPylation
DOI: 10.17632/pd3ph4zxvf.2
title: Taiwan earthquake catalog from 2012 to 2017

description: The zip file includes "cata.mat" and ReadMe.txt. The "cata.mat" file is the Taiwan earthquakes from 2012 to 2017 with ML&gt;=0. The *.mat means it is saved as a Matlab format. One variable "cata" included with 10 columns. From the first to last column, the format represents [year, month, day, hour, minute, second, latitude, longitude, depth, magnitude]. Note that the Earthquake Time is in UCT+8 (Taiwan time). Contact: redhouse6341@g.ncu.edu.tw Hong-Jia Chen
DOI: 10.6084/m9.figshare.8150555
title: Fig-2. Dicer suppression induces alterations in renal morphology and function.

description: Fig-2. Table A-C show at P30 and P50 smaller BW, KW and at P50 smaller KW/BW in cKO mice compared to other group. At P50, cKO mice present a significantly altered GFR and proteinuria compared to Ctr.
DOI: 10.6084/m9.figshare.8150555.v1
title: Fig-2. Dicer suppression induces alterations in renal morphology and function.

description: Fig-2. Table A-C show at P30 and P50 smaller BW, KW and at P50 smaller KW/BW in cKO mice compared to other group. At P50, cKO mice present a significantly altered GFR and proteinuria compared to Ctr.
DOI: 10.6084/m9.figshare.8150546
title: Fig-1. Dicer inactivation in mutant mice.

description: Fig-1 Panel A shows the genotyping of Dicer, Cre and Pax8 genes in mice either homozygote (cKO) or heterozygote (Het) for Dicer and control mice (Ctr). PCR of renal cortical tissues at P50 shows the Dicer allele floxed, wild type and excised forms. Cre is detected only in homozygous and heterozygous cKO mice as expected, while Pax8 is present constitutively in all the groups. Panel B shows western blot analysis of renal zones Cortex/OSOM, ISOM and IM samples from 50 day old mice. A reduced abundance of Dicer in cKO mice can be observed in all the zones. Normalization was carried out with GAPDH. Panel C shows qPCR analysis of Dicer mRNA. A substantial reduction of Dicer can be seen in cKO mice compared to Ctr. Data are expressed as mean ± sem.
DOI: 10.6084/m9.figshare.8150546.v1
title: Fig-1. Dicer inactivation in mutant mice.

description: Fig-1 Panel A shows the genotyping of Dicer, Cre and Pax8 genes in mice either homozygote (cKO) or heterozygote (Het) for Dicer and control mice (Ctr). PCR of renal cortical tissues at P50 shows the Dicer allele floxed, wild type and excised forms. Cre is detected only in homozygous and heterozygous cKO mice as expected, while Pax8 is present constitutively in all the groups. Panel B shows western blot analysis of renal zones Cortex/OSOM, ISOM and IM samples from 50 day old mice. A reduced abundance of Dicer in cKO mice can be observed in all the zones. Normalization was carried out with GAPDH. Panel C shows qPCR analysis of Dicer mRNA. A substantial reduction of Dicer can be seen in cKO mice compared to Ctr. Data are expressed as mean ± sem.
DOI: 10.6084/m9.figshare.8150528
title: BSP and qPCR data for Takifugu rubripes

description: This file includes the sequencing results of BSP analysis and qPCR data of amhr2 and pfcyp19a in male, female and pseudo male gonad for Takifugu rubripes. The BSP results were analyzed by DNAStar software.
DOI: 10.6084/m9.figshare.8150528.v1
title: BSP and qPCR data for Takifugu rubripes

description: This file includes the sequencing results of BSP analysis and qPCR data of amhr2 and pfcyp19a in male, female and pseudo male gonad for Takifugu rubripes. The BSP results were analyzed by DNAStar software.
DOI: 10.6084/m9.figshare.8149004
title: MOESM4 of A high-throughput amplicon-based method for estimating outcrossing rates

description: Additional file 3: Table S3. Results of statistical comparisons for the data shown in Fig. 6.
DOI: 10.6084/m9.figshare.8149004.v1
title: MOESM4 of A high-throughput amplicon-based method for estimating outcrossing rates

description: Additional file 3: Table S3. Results of statistical comparisons for the data shown in Fig. 6.
DOI: 10.6084/m9.figshare.8148998
title: MOESM3 of A high-throughput amplicon-based method for estimating outcrossing rates

description: Additional file 2: Table S2. Read statistics for the amplicons (see attached Excel sheet). The total number of fragments (i.e. paired reads) per sample is shown, as is the number and percentage of fragments mapped to the PCR amplicons, and the number and percentage of fragments used for haplotype calling. The latter excluded low-frequency fragments (
DOI: 10.6084/m9.figshare.8148998.v1
title: MOESM3 of A high-throughput amplicon-based method for estimating outcrossing rates

description: Additional file 2: Table S2. Read statistics for the amplicons (see attached Excel sheet). The total number of fragments (i.e. paired reads) per sample is shown, as is the number and percentage of fragments mapped to the PCR amplicons, and the number and percentage of fragments used for haplotype calling. The latter excluded low-frequency fragments (
DOI: 10.17632/4sprh5hxz5.1
title: VR pedagogical effect trial

description: The research study aimed to ascertain if using immersive VR games with traditional education enhances the learning gain, memory retention, and knowledge retention of students. This study wants to understand if VR games included with traditional learning methods can be a useful pedagogical tool. The methodology of this study consisted of analyzing learning gain, memory retention, and knowledge retention of the three different approaches to find out the best approach. The dataset is in Excel format and presents trials of 78 participants in three different learning approaches: Lecture-based approach, VR game-based approach, and a combination of lecture & VR game approach. The average percentage of learning gain, memory retention, and knowledge retention are calculated for each approach. The data was gathered through questionnaires that the participants filled before taking part in the experiment, immediately after the experiment, and three days after the experiment took place. The data was then placed into an excel file and grouped together. There are labels for each of the questions asked, the answers given by the participants and fields indicating if the participants answered the question correctly, remembered the question after 3 days, and whether the user retained knowledge in that question. For the pre-experiment questionnaire, there were 8 questions for all three approaches. There were 12 questions for the game-based approach, and the combined approach, whereas lecture-based had 8 questions for the post-experiment questionnaire.
DOI: 10.17632/75brhkh25r.1
title: A genome-wide hepatocyte CRISPR-CAS9 screen to identify host factors essential for optimal Plasmodium liver stage development.

description: To identify host genes critical for Plasmodium LS development, we used the GeCKOv2 sgRNA pool to generate a whole-genome knockout library in HepG2-CD81 cells (Sanjana et al., 2014; Shalem et al., 2014). HepG2-CD81 cells were transduced with lentivirus containing the pooled GeCKOv2 sgRNA library of 114,123 sgRNAs targeting 19,031 protein-coding genes (~6 sgRNAs/gene) and selected in puromycin for 5-7 days. To evaluate sgRNA diversity in the HepG2-CD81-GeCKOv2 library, we PCR-amplified the integrated sgRNA cassettes from genomic DNA extracted from transduced cells and subjected the amplified library to Illumina sequencing. At the gene level, sgRNAs targeting all but 15 of 19,031 (99.92%) protein-coding genes were observed. Twelve to fourteen days after post-transduction, we infected forty million puromycin-resistant cells with a green fluorescent protein (GFP) expressing-Plasmodium yoelii at a multiplicity of infection (MOI) of 0.3. After 24 h of infection, cells were sorted into infected and bystander cell populations by GFP signal intensity with fluorescence-activated cell sorting (FACS). Separately, a parallel culture of uninfected cells was also maintained to normalize the sgRNA frequency distributions. We obtained four independent biological replicates with library generation and sequencing occurring in parallel. Genes with significantly enriched sgRNAs were identified for both the bystander and infected populations when compared to uninfected cells. Cells that harbor genetic alterations restricting P. yoelii development (i.e., sgRNAs that target host genes important for infection) were expected to be enriched in the uninfected bin; we termed this group ‘putative positive regulators of infection’. We categorized sgRNAs enriched in the infected cells as ‘putative negative regulators of infection’. In this initial screen, we identified 242 sgRNAs that were statistically enriched in infected or bystander groups after accounting for multiple hypotheses. There were 67 sgRNAs significantly enriched in the infected cells compared to uninfected cells and 175 genes were significantly enriched in the bystander bin relative to the uninfected bin.
DOI: 10.6084/m9.figshare.8148953
title: Additional file 5: of Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps

description: Table S3. Differential expressed unigenes between vent and seep Shinkaia crosnieri. (XLSX 59 kb)
DOI: 10.6084/m9.figshare.8148953.v1
title: Additional file 5: of Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps

description: Table S3. Differential expressed unigenes between vent and seep Shinkaia crosnieri. (XLSX 59 kb)
DOI: 10.17632/x9k4ypjrmj.1
title: The Chen-Ouillon-Sornette seismo-electrical model

description: The data is simulated from the Chen-Ouillon-Sornette seismo-electrical model under diverse electrical parameters. The zip-files include the simulated slip time series and slip-induced electrical signals. Each text-file in the zip-file has two columns. The first column is the slip time series, and the second is the slip-induced electrical signals. The parameter settings are written in the ReadMe.txt.
DOI: 10.6084/m9.figshare.8148944
title: Additional file 4: of Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps

description: Table S2. The list of positively selected genes between vent and seep Shinkaia crosnieri. (XLSX 24 kb)
DOI: 10.6084/m9.figshare.8148944.v1
title: Additional file 4: of Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps

description: Table S2. The list of positively selected genes between vent and seep Shinkaia crosnieri. (XLSX 24 kb)
DOI: 10.17632/pc795c8w4p.1
title: Concentration of elements in diets from a mining area

description: This data was collected in a toxicovigilance effort to enable the baselining of exposures, risks and nutritional adequacy of elements in diets from the Western regions of Ghana, where gold mining prevalent. The data shows the communities and diet groups for antimony, arsenic, barium, cadmium, cobalt, lead, manganese, mercury, molybdenum, silver, strontium, tin, tungsten, uranium, vanadium, zirconium, calcium, copper, iron, potassium, selenium and zinc. The elemental concentrations in mg/kg were determined using XRF analyzer (Niton XL 3T 900 Model, Thermo Scientific, UK) after the diets were freeze dried.
DOI: 10.17632/y6496bvy5y.1
title: Review on heavy metals & MetS-2020

description: Supplemental information for a review including 1 table, 8 figures, and references.
DOI: 10.6084/m9.figshare.8148923
title: Additional file 1: of Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps

description: Table S1. Gene expression analyzed by qRT-PCR and RNA-Seq. (XLSX 11 kb)
DOI: 10.6084/m9.figshare.8148923.v1
title: Additional file 1: of Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps

description: Table S1. Gene expression analyzed by qRT-PCR and RNA-Seq. (XLSX 11 kb)
DOI: 10.6084/m9.figshare.8148791
title: Storytelling via Virtual Narrator Raw data.xlsx

description: Raw data used to analyze the persuasive effectiveness of storytelling via virtual narrator.The file contain the raw data from two sets of for each survey.<br>
DOI: 10.6084/m9.figshare.8148785
title: Matlab code and data

description: Matlab code and raw data
DOI: 10.6084/m9.figshare.8148779
title: LBP_UoN_CHS_2.dta

description: This dataset is in Stata format. It concerns information on low back pain collected (in 2017) from the teaching staff of the College of Health Sciences, University of Nairobi, Kenya. This was to fulfill one of the requirements for the MPH award.
DOI: 10.6084/m9.figshare.8148779.v1
title: LBP_UoN_CHS_2.dta

description: This dataset is in Stata format. It concerns information on low back pain collected (in 2017) from the teaching staff of the College of Health Sciences, University of Nairobi, Kenya. This was to fulfill one of the requirements for the MPH award.
DOI: 10.6084/m9.figshare.8148773
title: Appendix I - Questionnaire Survey

description: Questionnaire reference for JTP.
DOI: 10.6084/m9.figshare.8148761
title: APPENDIX I - Questionnaire.docx

description: Questionnaire for JTP
DOI: 10.6084/m9.figshare.8148761.v2
title: APPENDIX I - Questionnaire.docx

description: Questionnaire for JTP
DOI: 10.6084/m9.figshare.8148761.v1
title: APPENDIX I - Questionnaire.docx

description: Questionnaire for JTP
DOI: 10.6084/m9.figshare.8148746
title: Supplementary material for the article entitled "The advertisement call of Allobates niputidea (Anura: Aromobatidae)"

description: Supplementary materials for the article entitled "The advertisement call of Allobates niputidea (Anura: Aromobatidae)". This includes one table and two figures. The table shows the measured traits for Allobates niputidea, and a review of those traits in other Allobates species in Colombia, and Colostethus inguinalis (sympatric species) for comparison. In the figure 1, we present the distribution of A. niputidea in Colombia and the study area. In the figure 2, we present the structure of a complete call of A. niputidea. Additionally, we include a pdf with the legends of these supplementary materials and references.
DOI: 10.6084/m9.figshare.8148746.v2
title: Supplementary material for the article entitled "The advertisement call of Allobates niputidea (Anura: Aromobatidae)"

description: Supplementary materials for the article entitled "The advertisement call of Allobates niputidea (Anura: Aromobatidae)". This includes one table and two figures. The table shows the measured traits for Allobates niputidea, and a review of those traits in other Allobates species in Colombia, and Colostethus inguinalis (sympatric species) for comparison. In the figure 1, we present the distribution of A. niputidea in Colombia and the study area. In the figure 2, we present the structure of a complete call of A. niputidea. Additionally, we include a pdf with the legends of these supplementary materials and references.
DOI: 10.6084/m9.figshare.8148728
title: Spatial data and supporting information for a Philippines case study on social equity in marine systematic conservation planning

description: This dataset includes supporting information and spatial data for a Philippines case study on social equity in marine systematic conservation planning (SCP). The study focuses on three dimensions of social equity (recognition, procedure, and distribution) in relation to planning marine protected areas (MPAs) under a SCP framework. It documents how social equity dimensions can be addressed within the planning stages of SCP and compares three planning scenarios to investigate how recognition and procedure equity can impact MPA plans in terms of distribution equity, ecological representation, and spatial efficiency. The study region is located in Sogod Bay in Southern Leyte, Philippines and encompasses the coastal waters of six municipalities: Pintuyan, San Francisco, Liloan, Malitbog, Padre Burgos, and Limasawa. Biodiversity and fishery data were collected using remote sensing and participatory mapping methods, respectively. The data were integrated into the spatial prioritization tool, Marxan with Zones, to develop and compare three planning scenarios. <br> The <b>supporting information file</b> contains supplemental information on (1) biodiversity data and remote sensing analyses; (2) fishery data and participatory mapping workshops; (3) existing MPA system in the study region; and (4) Marxan with Zones analyses including (a) summary tables of the final Marxan with Zones analysis of each planning scenario, (b) input and output files of Marxan with Zones analyses by scenario, and (c) maps of Marxan with Zones outputs under the three scenarios. <br> The <b>spatial data file</b> includes SHP and KML files on (1) the administrative boundaries for (a) barangays/communities, (b) municipalities, and (c) municipal waters in the study region; (2) (a) the amount of each biodiversity feature in each planning unit, (b) the distribution of coastal habitats derived from a remote sensing analysis of three WorldView-2 images, (c) areas with cloud cover where habitat classes could not be derived from the remote sensing analysis; (d) mangrove data derived from secondary data sources; (3) the amount of each fishery feature in each planning unit at (a) a barangay/community scale and (b) a municipal scale; and (4) (a) the location and attribute information on existing MPAs in 2015, (b) planning units selected in the MPA zone of the “best” Marxan with Zones solution (lowest score of 100 runs) by scenario, and (c) planning unit selection frequencies for the MPA zone by scenario.<br> <b>***Note***</b> Barangay/community names have been replaced with codes to maintain the confidentiality agreement of this study.