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DOI: 10.6084/m9.figshare.5734452.v1
title: Physiological quality of tomato seeds treated with polymers in combination with paclobutrazol

description: Abstract: In tomatoes production system, etiolation is the main cause of seedlings loss. Some inhibitors, such as paclobutrazol (PBZ), have been used to reduce this problem. This study aimed to evaluate the effect of different doses of paclobutrazol, with and without polymer association, at different degrees of permeability, on the physiological quality of seeds. Seeds of two tomato cultivars, Ozone and Paronset, were treated with four concentrations of paclobutrazol (0, 0.004, 0.008 and 0.016 g mL a.i.10 g seeds-1), and combined with three fixatives (water, P1 and P2). After the treatments, germination test, germination speed index test and cold test were carried out, and after five months of seed storage, the analyzes were repeated. The increase in PBZ dose reduces both germination percentage and germination speed index. After storage, the product acts differently on seed germination. The synthetic polymers, in the dose used in this study, are effective in PBZ fixation on the seeds coat, but do not reduce the harmful effect of PBZ on seed germination.
DOI: 10.6084/m9.figshare.5670667.v1
title: Changes in desiccation tolerance and respiratory rates of immature Caesalpinia echinata Lam. Seeds

description: Abstract: Seed storage is an important tool for ex situ conservation. Orthodox seeds can be stored for long periods, but recalcitrant seeds generally only for short periods. There is wide variation in the degree of desiccation tolerance between orthodox and recalcitrant seeds, leading authors to suggest levels of recalcitrance and, more recently, that there are variations in the stage of maturity at seed dispersal. Thus, recalcitrant behavior would be a result of premature dispersal from the mother plant. In this study, we sought to establish physiological relationships between different stages of development of immature orthodox Caesalpinia echinata seeds and the stages described for recalcitrant seeds to verify similarity of behavior. Therefore, we analyzed the desiccation tolerance of seeds collected at different ages, with and without PEG treatment, at different levels of drying (40%, 30%, 20% and 10% water content, wet basis). Changes in water potential, germination, vigor, and respiratory rates of the seeds were analyzed, and the results showed that: 1) desiccation tolerance increased as maturation proceeded; 2) PEG treatment did not induce desiccation tolerance; and 3) PEG treatment decreased the seed oxidation rate, which is the main factor in rapid seed deterioration of C. echinata in storage.
DOI: 10.6084/m9.figshare.5671261.v1
title: Exploratory studies for cryopreservation of Coffea arabica L. seeds

description: Abstract: Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.
DOI: 10.6084/m9.figshare.5671258.v1
title: Assessment of squash seed vigor using computerized image analysis

description: Abstract: The objective of this study was to determine the efficacy of the SVIS® (Seed Vigor Imaging System) to determine squash seed vigor evaluated at different stages of maturation. ‘Jabras’ hybrid seeds, resulting from a cross between Cucurbita maxima (female parent) and Cucurbita moschata (male parent), were used in this study. Seeds extracted from fruit at different maturation stages (harvested at 15, 30, 45, 60 and 75 days after anthesis and stored or not for 20 days after each harvest) were subjected to the following determinations: 100 seed weight, germination, saturated salt accelerated aging, and SVIS®. This computerized system provided data on indexes of seedling vigor and uniformity of seedling development and on seedling growth. Results from SVIS® were similar to information from the other vigor tests performed in this study, detecting significant differences in performance among seeds during the maturation process. This indicates that SVIS® is a reliable alternative for obtaining consistent information on the physiological potential of squash seeds.
DOI: 10.6084/m9.figshare.5645188
title: Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

description: Abstract: This present study aimed to assess seed quality of popcorn seedlings subjected to low temperatures by examining their physiological changes, enzymes, and images. We used a completely randomized design with four replications in a factorial arrangement with four hybrids (P618, AP6002, AP8202, AP8203) and five temperatures (10, 13, 16, 19 and 25 °C). Their physiological quality was assessed through radicle protrusion, germination, dry mass of seedlings and emergence. We have assessed the images of seedlings through their hypocotyl and root lengths; and through rates of automatic force, uniformity and growth, provided by Groundeye. The isozyme expressions were determined for the catalase (CAT - IUBMB: EC 1.11.1.6), alcohol dehydrogenase (ADH - IUBMB: EC 1.1.1.1), malate dehydrogenase (MDH - IUBMB: EC: 1.1.1.37) and α-amylase (α-AMY - IUBMB: EC 3.2.1.1). Results show that low temperatures cause a negative effect on seed physiological quality in enzyme expression of CAT, ADH, MDH and α-AMY and on the performance of seedlings. These alterations compromise seed quality. Hybrids AP6002 and AP8203 should be recommended for sowing at up to 16 °C. The Groundeye software was efficient in the analysis of popcorn seedlings and in for the assessment of seed quality when submitted to low temperatures.
DOI: 10.6084/m9.figshare.5645188.v1
title: Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

description: Abstract: This present study aimed to assess seed quality of popcorn seedlings subjected to low temperatures by examining their physiological changes, enzymes, and images. We used a completely randomized design with four replications in a factorial arrangement with four hybrids (P618, AP6002, AP8202, AP8203) and five temperatures (10, 13, 16, 19 and 25 °C). Their physiological quality was assessed through radicle protrusion, germination, dry mass of seedlings and emergence. We have assessed the images of seedlings through their hypocotyl and root lengths; and through rates of automatic force, uniformity and growth, provided by Groundeye. The isozyme expressions were determined for the catalase (CAT - IUBMB: EC 1.11.1.6), alcohol dehydrogenase (ADH - IUBMB: EC 1.1.1.1), malate dehydrogenase (MDH - IUBMB: EC: 1.1.1.37) and α-amylase (α-AMY - IUBMB: EC 3.2.1.1). Results show that low temperatures cause a negative effect on seed physiological quality in enzyme expression of CAT, ADH, MDH and α-AMY and on the performance of seedlings. These alterations compromise seed quality. Hybrids AP6002 and AP8203 should be recommended for sowing at up to 16 °C. The Groundeye software was efficient in the analysis of popcorn seedlings and in for the assessment of seed quality when submitted to low temperatures.
DOI: 10.6084/m9.figshare.5670922
title: Differential incidence of the lemma on seed germination among different Paspalum dilatatum genotypes

description: Abstract: Paspalum dilatatum Poir. is a promising perennial summer grass forage for temperate regions, but among other factors, slow stand establishment has hindered its adoption. One of the reasons may be seed dormancy. Intraspecific variability in seed dormancy has been reported in P. dilatatum, but the mechanisms underlying this variability remain unclear. In this paper, we focus on the role of seed external covering structures on germination, particularly the lemma. Seeds of apomictic and sexual biotypes of P. dilatatum were subjected to acid scarification and removal of the lemma to study their germination, imbibition rate and sensitivity to ABA. Seeds without lemma showed higher germination than other treatments in dormant genotypes. We found that the lemma delayed but did not prevent water uptake in any genotype. Comparing sexual biotypes, P. dilatatum subsp. flavescens showed higher lemma-imposed dormancy than P. dilatatum “Virasoro”, and also higher seed dormancy at the caryopsis level. The thickness of the lemma was not different between these biotypes, so this trait did not explain differences in dormancy. Intraspecific variability of seed dormancy in this species may be attributable to the lemma incidence, however differences in the caryopsis component of seed dormancy should be considered.
DOI: 10.6084/m9.figshare.5734245
title: Osmotic stress on genetically transformed tobacco plant seeds

description: Abstract: Salinity and water deficit limit the productivity of several crops; thus, studies related to the genetic transformation of seeds in a model plant, such as tobacco, can be an alternative to minimize negative impacts caused by environmental conditions. The purpose of this work was to evaluate the tolerance to osmotic stress of seeds from genetically transformed tobacco plants, with the introduction of the proline-synthesizer gene (p5csf129a), under salinity and water deficit conditions. To do so, five events with differences in proline content were selected, ranging from 0.70 to 10.47 µmoles.g-1 of fresh mass. The used saline concentrations were: zero (distilled water); 50; 100; 150 and 200 mmol.L-1 of NaCl, whereas for the water deficit, simulated with PEG 6000, the following osmotic potentials were used: zero (distilled water); -0.2; -0.4; -0.6 and -0.8 MPa. Each tested treatment was evaluated through germination, first germination count and germination speed index tests. It is possible to conclude that seeds from genetically transformed tobacco plants with overexpression of the gene p5csf129a, a proline synthesizer, are more tolerant to osmotic stresses. Tabacco seeds with a proline content of 10.47 µmol.g-1 showed a better perfomance, revealing higher physiological potential.
DOI: 10.6084/m9.figshare.6235202
title: Potential of transmission of Pyricularia graminis-tritici from plant to seed and from seed to seedling in wheat genotypes with different degrees of blast resistance

description: Abstract: Transmission studies of Pyricularia graminis-tritici by wheat seed can help establishing pathogen tolerance standards in crops. Four genotypes, each one with different responses to blast, were inoculated with five volumes of fungal suspension (1,5x105 spores.mL-1), in order to obtain 0, 5, 10, 20 and 30% of plants inoculated in the experimental unit. The potential of transmission of P. graminis-tritici from plant to seed was evaluated by the incidence of the fungus in the seeds produced. Under controlled conditions, the transmission rate of the fungus from seed to seedling was evaluated. Blast incidence in field allowed a high incidence of P. graminis-tritici in the seeds, especially in the genotypes considered susceptible. The transmission of fungus from the seeds to seedlings occurred 7, 14 and 21 days after sowing, at low rates. There was a relationship between the presence of blast in field and the incidence of P. graminis-tritici in seeds produced by the genotypes BRS 264, VI 98053, CD 116 and CD 104. Inoculation of 5, 10, 20 and 30% of plants with fungus can generate a high incidence of the pathogen in field and in the seeds produced, but it doesn`t guarantee a high transmission rate from seed to seedling, which is low under controlled conditions.
DOI: 10.6084/m9.figshare.7515029
title: Luminosity and sowing depth in the emergence and development of passion fruit seedlings

description: Abstract: The propagation of passion fruit in commercial orchards is carried out by seeds. However, passion fruit seed germination occurs irregularly. The goal of this study was to evaluate the emergence and development of passion fruit seedlings under different luminosities and sowing depths. The experimental design was completely randomized with six replications of 25 seeds, with a 3 x 5 factor scheme, in which three light conditions (absence of light, presence of 12 h light and 12 h dark and the presence of light for 24 h) were combined with five sowing depths (1, 2, 3, 4 and 5 cm). The emergence test was conducted in a BOD-type germination chamber, with alternating temperatures of 20-30 °C, at intervals of 12 h and the established luminosities. The evaluated characteristics were the emergence percentage for weekly counts, the emergence speed index for 28 days; the total shoot and root length on evaluation day 28 and the individual dry weight. The lack of luminosity accelerates the emergence of passion fruit seedlings, but it produces low quality seedlings. However, the alternation of luminosity associated to a lower sowing depth helps both the emergence and the quality of seedlings produced from passion fruit 14 days after sowing.
DOI: 10.6084/m9.figshare.5734452
title: Physiological quality of tomato seeds treated with polymers in combination with paclobutrazol

description: Abstract: In tomatoes production system, etiolation is the main cause of seedlings loss. Some inhibitors, such as paclobutrazol (PBZ), have been used to reduce this problem. This study aimed to evaluate the effect of different doses of paclobutrazol, with and without polymer association, at different degrees of permeability, on the physiological quality of seeds. Seeds of two tomato cultivars, Ozone and Paronset, were treated with four concentrations of paclobutrazol (0, 0.004, 0.008 and 0.016 g mL a.i.10 g seeds-1), and combined with three fixatives (water, P1 and P2). After the treatments, germination test, germination speed index test and cold test were carried out, and after five months of seed storage, the analyzes were repeated. The increase in PBZ dose reduces both germination percentage and germination speed index. After storage, the product acts differently on seed germination. The synthetic polymers, in the dose used in this study, are effective in PBZ fixation on the seeds coat, but do not reduce the harmful effect of PBZ on seed germination.
DOI: 10.6084/m9.figshare.7515029.v1
title: Luminosity and sowing depth in the emergence and development of passion fruit seedlings

description: Abstract: The propagation of passion fruit in commercial orchards is carried out by seeds. However, passion fruit seed germination occurs irregularly. The goal of this study was to evaluate the emergence and development of passion fruit seedlings under different luminosities and sowing depths. The experimental design was completely randomized with six replications of 25 seeds, with a 3 x 5 factor scheme, in which three light conditions (absence of light, presence of 12 h light and 12 h dark and the presence of light for 24 h) were combined with five sowing depths (1, 2, 3, 4 and 5 cm). The emergence test was conducted in a BOD-type germination chamber, with alternating temperatures of 20-30 °C, at intervals of 12 h and the established luminosities. The evaluated characteristics were the emergence percentage for weekly counts, the emergence speed index for 28 days; the total shoot and root length on evaluation day 28 and the individual dry weight. The lack of luminosity accelerates the emergence of passion fruit seedlings, but it produces low quality seedlings. However, the alternation of luminosity associated to a lower sowing depth helps both the emergence and the quality of seedlings produced from passion fruit 14 days after sowing.
DOI: 10.6084/m9.figshare.5734245.v1
title: Osmotic stress on genetically transformed tobacco plant seeds

description: Abstract: Salinity and water deficit limit the productivity of several crops; thus, studies related to the genetic transformation of seeds in a model plant, such as tobacco, can be an alternative to minimize negative impacts caused by environmental conditions. The purpose of this work was to evaluate the tolerance to osmotic stress of seeds from genetically transformed tobacco plants, with the introduction of the proline-synthesizer gene (p5csf129a), under salinity and water deficit conditions. To do so, five events with differences in proline content were selected, ranging from 0.70 to 10.47 µmoles.g-1 of fresh mass. The used saline concentrations were: zero (distilled water); 50; 100; 150 and 200 mmol.L-1 of NaCl, whereas for the water deficit, simulated with PEG 6000, the following osmotic potentials were used: zero (distilled water); -0.2; -0.4; -0.6 and -0.8 MPa. Each tested treatment was evaluated through germination, first germination count and germination speed index tests. It is possible to conclude that seeds from genetically transformed tobacco plants with overexpression of the gene p5csf129a, a proline synthesizer, are more tolerant to osmotic stresses. Tabacco seeds with a proline content of 10.47 µmol.g-1 showed a better perfomance, revealing higher physiological potential.
DOI: 10.6084/m9.figshare.5671261
title: Exploratory studies for cryopreservation of Coffea arabica L. seeds

description: Abstract: Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.
DOI: 10.6084/m9.figshare.5734389
title: Temperature and seed moisture content affect electrical conductivity test in pea seeds

description: Abstract: Several factors affect the electrical conductivity test efficiency, with emphasis given to the initial water content and the temperature during imbibition. This study aimed to evaluate the effect of the initial water content of pea seeds and the temperature on the electrical conductivity test efficiency. Six lots of ‘Mikado’ pea were used, which were previously tested for initial characterization. In the first trial, based on the initial value of the samples, the seed moisture content was adjusted to 9, 11, 13 and 15% and then the seeds were submitted to the electrical conductivity test. In the second trial, the water for seed imbibition was kept for 24 hours at 10, 15, 20, 25 and 30 ºC, and after that time the seeds were added and soaked for other 24 hours at the temperatures already mentioned. It was observed that initial seed moisture content and water temperature during imbibition influenced the results of the electrical conductivity test. This test should be carried out with distilled water, previously maintained for 24 hours at 25 °C for temperature stabilization. Then, the seeds with seed moisture content between 11 and 15% should be put to soak at 25 °C, and maintained at this temperature for 24 hours.
DOI: 10.6084/m9.figshare.5734449.v1
title: Controlled deterioration test and use of the Seed Vigor Imaging System (SVIS ® ) to evaluate the physiological potential of crambe seeds

description: Abstract: Vigor tests are important tools for verifying the physiological potential of seed lots; however, various aspects can interfere in the consistency of the test results. The search for less subjective procedures has drawn the interest of researchers. The aim of this study was to adapt the methodology of the controlled deterioration test and verify the efficiency of the Seed Vigor Imaging System (SVIS ® ) to evaluate the vigor of crambe seeds. Initially, seed moisture content was determined and germination and vigor were evaluated in five seed lots. For the controlled deterioration test, the seed moisture content was adjusted to 18%, 20%, and 22%, and the results were interpreted at four and five days after sowing. The controlled deterioration test should be performed with initial adjustment of seed moisture content to 18%, and the germination test should be evaluated on the fifth day after sowing. SVIS ® provided information on the vigor index and the length and uniformity of seedling development. Computerized image analysis is effective in evaluating the vigor of crambe seeds, and the uniformity of seedling development parameter offers the most consistent data.
DOI: 10.6084/m9.figshare.5734458
title: Seed quality of Amburana cearensis (Allemão) A.C. Sm. (Fabaceae) is influenced by storage condition

description: Abstract: The aim of this study was to evaluate the effects of storage conditions on the germination of A. cearensis seeds. The experimental design was completely randomized into split plots over time with four replicates. The storage conditions of the airtight containers in the refrigerator and laboratory, paper bags in the laboratory and liquid nitrogen were assessed for 27 months. In the laboratory, we evaluated the germination, the germination rate, uniformity of germination, and total soluble and reducing sugars in the radicle. In the greenhouse, we evaluated seedling emergence, emergence rate and height of 30-day-old seedlings. Seeds stored in the refrigerator maintained a high initial germination rate, which decreased from the 21st month. Seeds stored in paper bags in the laboratory showed low emergence and small seedlings. Total soluble sugars and reducing sugars were mobilized when the seeds were stored at low temperatures. Thus, it is not advisable to store A. cearensis seeds in a laboratory environment without airtight containers. A. cearensis seeds kept in a refrigerated environment maintained their viability for at least two years.