cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.34816/efa.archives.514474
title: Delphes-PV_A73-Adyton

description: Référence : PV_A73<br />Publication(s) : FD II, Le Temple d'Apollon du IVe siècle, p. 317<br />Domaine : Architecture<br />
DOI: 10.34816/efa.archives.514473
title: Delphes-PV_A169-Adyton

description: Référence : PV_A169<br />Publication(s) : FD II, Le Temple d'Apollon du IVe siècle, p. 312, fig. 11.20<br />Domaine : Architecture<br />
DOI: 10.48323/6gass-fj364
title: MS_HFX_2021_September

description: Q Exactive HF-X + Nano U3000
-TMair Ac enrichment
-Histone DTT
-Standards
Q Exactive HF-X + Vanquish Flex
-Standards
DOI: 10.48323/jrz3k-zzf64
title: MS_Lumos_2022_September
DOI: 10.34816/efa.archives.514472
title: Delphes-PV_A170-Adyton

description: Référence : PV_A170<br />Publication(s) : FD II, Le Temple d'Apollon du IVe siècle, p. 310, fig. 11.18<br />Domaine : Architecture<br />
DOI: 10.48323/px6qa-29c92
title: MS_Lumos_2021_May
DOI: 10.7483/opendata.cms.7fwn.u9ym
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-300_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.34816/efa.archives.514471
title: Delphes-PV_A168-Adyton

description: Référence : PV_A168<br />Publication(s) : FD II, Le Temple d'Apollon du IVe siècle, p. 311, fig. 11.19<br />Domaine : Architecture<br />
DOI: 10.48323/232b2-v9s05
title: MS_HFX_2021_February
DOI: 10.34816/efa.archives.514449
title: Delphes-47159-Sujet : gravure, Delphes, site, vue d'ensemble, Datation : 1676

description: Référence : 47159<br />Publication(s) : La Redécouverte de Delphes, p. 20, fig. 3<br />Domaine : Architecture<br />
DOI: 10.48323/cj5bw-a7h41
title: MATURITY MODEL FOR DIGITAL BUSINESS ECOSYSTEMS FROM AN IT PERSPECTIVE - APPENDIX

description: Digital transformation impacts longstanding business models and enables enterprises to create new ones. This transformation leads to increased competition that forces enterprises to compete, not only between companies but also between the entire supply chain and business networks. The emerging concept of digital business ecosystems (DBE) allows enterprises to concentrate on network co-creation and co-evolution of bundled services and products across enterprise boundaries. This exploratory study introduces a maturity model derived from existing DBEs. Based on employing a design science methodology, we reviewed 22 scientific publications, interviewed 28 senior experts from practice and derived a maturity model from the results. We applied the maturity model through an online survey to 29 DBEs from different industry sectors and compared it with 22 maturity assessment approaches from other domains. The maturity model enables researchers to compare and assess existing DBEs and helps practitioners to identify areas for improvement in the collaboration within DBEs.
DOI: 10.7483/opendata.cms.mtvw.9ytn
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-400_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.34816/efa.archives.514189
title: Delphes-C1070-023-Inscription, épiclèse de Poséidon

description: Référence : C1070-023<br />Publication(s) : BCH 126 2002, p. 56, fig. 1<br />Domaine : Épigraphie<br />
DOI: 10.7483/opendata.cms.h9q0.qrk3
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-400_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.34816/efa.archives.513705
title: Delphes-S2339-Inscription, décret, estampage

description: Référence : S2339<br />Publication(s) : FD III 3, pl. 9, fig. 1<br />Domaine : Épigraphie<br />
DOI: 10.48323/ztqmy-qzd91
title: MS_Lumos_2022_October
DOI: 10.34816/efa.archives.513704
title: Delphes-S2338-Inscription, décret, estampage

description: Référence : S2338<br />Publication(s) : FD III 3, pl. 9, fig. 2<br />Domaine : Épigraphie<br />
DOI: 10.48323/5q3xd-ayy93
title: Dataset Stability of selected exhaled breath volatiles stored in Tenax® TA adsorbent tubes at -80 °C

description: Agilent GC/MS records
DOI: 10.34816/efa.archives.513437
title: Delphes-2267-Figure en ronde bosse, reconstitution, Matériau : marbre, calcaire, Support : base de la colonne d'Acanthe, pilier, Décor : femme, danseuse, guerrier, Technique : ronde bosse

description: Référence : 2267<br />Publication(s) : La Redécouverte de Delphes, p. 209, fig. 106 B<br />Domaine : Architecture; Sculpture<br />
DOI: 10.48323/p4tk6-qy653
title: MS_Lumos_2020_April

description: MS Raw Data collected at Obitrap Fusion Lumos in April 2020 at the Institute of Biochemistry, University of Innsbruck
This record contains Proteomics of Marcel Kwiatkowski (UIBK) & AcylCoAs (M. Hotze UIBK) &  Metabolite measurements (M. Hotze, UIBK).
DOI: 10.7483/opendata.cms.uw22.z2om
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-500_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.34816/efa.archives.510023
title: Delphes-45892-Figure en ronde bosse, vue d'ensemble, homme, Sisyphos II, Sisyphos I, Daochos I, Agelaos, Agias, Aknonios, marbre de Paros

description: Référence : 45892<br />Publication(s) : BCH 105, 1981, p. 754, fig. 74<br />Période(s) : Classique; Antiquité<br />Domaine : Sculpture<br />
DOI: 10.48323/8awg6-ggc68
title: Automated Liquid Handling Extraction and Rapid Quantifica-tion of Underivatized Amino Acids and Tryptophan Metabo-lites from Human Serum and Plasma Using Dual-column U(H)PLC-MRM-MS and its Application to Prostate Cancer Study
DOI: 10.34816/efa.archives.509748
title: Delphes-2266-Figure en ronde bosse, colonne, reconstitution, Matériau : plâtre, Support : base de la colonne d'Acanthe, Décor : femme, danseuse

description: Référence : 2266<br />Publication(s) : La Redécouverte de Delphes, p. 276, fig. 169B ; FD IV, 8.2, p. 528, fig. 6a<br />Domaine : Architecture; Sculpture<br />
DOI: 10.48323/sadqv-xt457
title: MS_Lumos_2019_September
DOI: 10.7483/opendata.cms.6931.uw5k
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-500_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.34816/efa.archives.509478
title: Delphes-N336-004-Figure en ronde bosse, danseuses, colonne d'Acanthe, omphalos

description: Référence : N336-004<br />Publication(s) : FD IV, 8.2, p. 536, fig. 11<br />Période(s) : Classique; Antiquité; Romain; Impérial<br />Domaine : Architecture; Sculpture<br />
DOI: 10.34816/efa.archives.509441
title: Delphes-N336-034-Colonne d'Acanthe, base

description: Référence : N336-034<br />Publication(s) : FD IV, 8.2, p. 545, fig. 17<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.48323/tkncd-08762
title: MS_Lumos_2022_June
DOI: 10.34816/efa.archives.509440
title: Delphes-N336-033-Colonne d'Acanthe, base

description: Référence : N336-033<br />Publication(s) : FD IV, 8.2, p. 545, fig. 17<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.7483/opendata.cms.k6j7.cu4a
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-600_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.48323/gywz5-x6n56
title: Foehn reconstruction for station Ellbögen and Innsbruck

description: Supplementary material for research article "Stauffer R., Zeileis A., Mayr G.J. Long-term foehn reconstruction combining unsupervised and supervised learning. International Journal of Climatology, The Royal Meteorological Society." containing data for station Ellbögen and Innsbruck (Austria).`ERA5predictors.txt` contains (pseudo-)code for all direct and derived ERA5 variables used in the study. The two ZIP archives `foehnix_Ellboegen.zip` and `foehnix_Innsbruck.zip` contain one comma separated value (CSV) file each, with the high-resolution in-situ observations from the corresponding automated weather stations as used for foehn classification (unsupervised learning; foehnix). The two ZIP archives `reconstruction_Ellboegen.zip` and `reconstruction_Innsbruck.zip` contain two CSV files with the hourly reconstructed time series (supervised learning) from 1940 to 2022. All CSV files contain a header with meta information regarding the format as well as the content of the corresponding file. All data provided is available under the Creative Commons Attribution 4.0 license (see also CSV meta information).
DOI: 10.34816/efa.archives.509439
title: Delphes-N336-032-Colonne d'Acanthe, base

description: Référence : N336-032<br />Publication(s) : FD IV, 8.2, p. 545, fig. 17<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.34816/efa.archives.509437
title: Delphes-N336-027-Colonne d'Acanthe, base

description: Référence : N336-027<br />Publication(s) : FD IV, 8.2, p. 548, fig. 18<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.48323/8kjeg-mcx57
title: XML-Corpus Diss A. Schönherr

description: Corpus of Website articles that were analysed in https://digital.obvsg.at/ulbtirolhs/content/titleinfo/6572630
DOI: 10.7483/opendata.cms.07f5.h9m8
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-600_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.15468/dl.253qfh
title: Occurrence Download

description: A dataset containing 820 species occurrences available in GBIF matching the query:
{
 "and" : [
 "CoordinateUncertaintyInMeters is less than or equal to 5000.0",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Discoglossus galganoi Capula, Nascetti, Lanza, Bullini & Crespo, 1985, Discoglossus jeanneae Busack, 1986)",
 "Year 1980-2023"
 ]
}

 The dataset includes 820 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0228142-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.7483/opendata.cms.z1ph.1hgv
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-60_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.34816/efa.archives.509436
title: Delphes-N336-026-Colonne d'Acanthe, base

description: Référence : N336-026<br />Publication(s) : FD IV, 8.2, p. 554, fig. 22 ; p. 566, fig. 32<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.48323/3j8v6-smr19
title: Analysis of the phosphatidylinositol fatty acid composition in α-T-13′-COOH-treated macrophages

description: RAW264.7 cells were incubated with vehicle (DMSO, 'w/o') or 0.5 or 5.0 µM α-T-13′-COOH for 24 h. The fatty acid distribution of phosphatidylinositol was then analyzed by UPLC-MS/MS.
Raw analyst files (.wiff and .wiff.scan) of the UPLC-MS/MS results were uploaded, together with an Excel file for the sample list.
The methods and results were published in Liao et al., Int J Mol Sci, 2023 May 25;24(11):9229. doi: 10.3390/ijms24119229.
Lipids were extracted from RAW264.7 cell pellets by the successive addition of methanol, PBS (pH 7.4), chloroform, and saline (final ratio: 34:14:35:17). After the evaporation of the organic solvent, the remaining lipid fraction was dissolved in methanol, stored at −20 °C, and analyzed by UPLC-MS/MS. Internal standards: 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidyl-ethanolamine (DMPE).
Phospholipids (PC, PE, PI, PS, PG) were separated on an Acquity UPLC BEH C8 column (130 Å, 1.7 μm, 2.1 × 100 mm; Waters, Milford, MA, USA) using an Acquity UPLC system (Waters), which was coupled to a QTRAP 5500 mass spectrometer (Sciex, Framingham, MA, USA) equipped with a Turbo V Ion Source and an electrospray ionization probe. Chromatographic separation was performed at a flow rate of 0.75 mL/min and at a column temperature of 45 °C. The mobile phase was composed of eluent A (acetonitrile/water, 95/5, with 2 mM ammonium acetate) and eluent B (water/acetonitrile, 90/10, with 2 mM ammonium acetate). The gradient was ramped from 70% to 80% A within 5 min and to 100% A within 2 min, followed by isocratic elution for another 2 min. Eluted phospholipids were detected upon the fragmentation of parental ions (PC: [M+OAc]−, all other phospholipids: [M-H]−) to fatty acid anions derived from sn-1 and sn-2 positions by multiple reaction monitoring using a QTRAP 5500 mass spectrometer. The ion spray voltage was set to −4500 V, the curtain gas to 30 psi, the collision gas to medium, and the heated capillary temperature to either 350 °C (PC), 500 °C (PI), 550 °C (PS, PG), or 650 °C (PE). The sheath gas pressure was set to 45 (PS) or 55 psi (PC, PE, PI, PG) and the auxiliary gas pressure was set to either to 75 psi (PC, PE, PI, PG) or 80 psi (PS). The declustering potential was set to −40 V (PS), −44 V (PC), −45 V (PG), or −50 V (PE, PI), the entrance potential to −10 eV (PC; PE, PI, PS, PG), the collision energy to −38 eV (PE), −46 eV (PC), −52 eV (PG), −56 eV (PS), or −62 eV (PI), and the collision cell exit potential to −11 V (PC, PI), −12 V (PE), −18 V (PG), or −20 V (PS).
The instruments were either operated with Analyst 1.6.2 (QTRAP 5500, Sciex) or Analyst 1.7.1 (QTRAP 6500+, Sciex).
DOI: 10.34816/efa.archives.509435
title: Delphes-N336-025-Colonne d'Acanthe, base

description: Référence : N336-025<br />Publication(s) : FD IV, 8.2, p. 554, fig. 22<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.34816/efa.archives.509434
title: Delphes-N336-024-Colonne d'Acanthe, base

description: Référence : N336-024<br />Publication(s) : FD IV, 8.2, p. 554, fig. 22<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.48323/p4q53-8vt67
title: Effect of curcuminoids on DPPH radical scavenging

description: Scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals by 3b, cysteine, and ascorbic acid (2–200 µM).
Raw data including the absolute values (absorbance subtract ethanol blank) and normalized data (% of control) of all individual experiments was uploaded.
The methods and results were published in Rao et al., Biochem Pharmacol. 2022 Sep:203:115202. doi: 10.1016/j.bcp.2022.115202 
Vehicle (ethanol), compound 3b, cysteine hydrochloride (reference compound, Merck) or ascorbic acid (reference compound, Caesar & Loretz GmbH, Hilden, Germany) were incubated with 2,2-diphenyl-1-picrylhydrazyl (Merck; 50 µM) under shaking in the dark. After 30 min, the absorbance was measured at 520 nm using a Multiskan Spectrum Microplate Reader (Thermo Fisher Scientific).
DOI: 10.48323/eh4pa-f5y22
title: Analysis of the triglyceride fatty acid composition in α-T-13′-COOH-treated macrophages

description: RAW264.7 cells were incubated with vehicle (DMSO, 'w/o') or 0.5 or 5.0 µM α-T-13′-COOH for 24 h. The fatty acid distribution of triglyceride was then analyzed by UPLC-MS/MS.
Raw analyst files (.wiff and .wiff.scan) of the UPLC-MS/MS results were uploaded, together with an Excel file for the sample list.
The methods and results were published in Liao et al., Int J Mol Sci, 2023 May 25;24(11):9229. doi: 10.3390/ijms24119229 
Lipids were extracted from RAW264.7 cell pellets by the successive addition of methanol, PBS (pH 7.4), chloroform, and saline (final ratio: 34:14:35:17). After the evaporation of the organic solvent, the remaining lipid fraction was dissolved in methanol, stored at −20 °C, and analyzed by UPLC-MS/MS. Internal standards: 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidyl-ethanolamine (DMPE).
TGs were separated on an Acquity UPLC BEH C8 column (130 Å, 1.7 μm, 2.1 × 100 mm; Waters) using either an Acquity UPLC system (Waters), which was coupled to a QTRAP 5500 mass spectrometer (Sciex) equipped with a Turbo V Ion Source and an electrospray ionization probe, or an ExionLC AD UHPLC system (Sciex), which was coupled to a QTRAP 6500+ mass spectrometer (Sciex) equipped with an IonDrive Turbo V Ion Source and an electrospray ionization probe. In brief, both LC systems were operated at a flow rate of 0.75 mL/min and a column temperature of 45 °C. The mobile phase consisted of eluent A (acetonitrile/water, 95/5, with 2 mM ammonium acetate) and eluent B (isopropanol). For the separation of TGs, eluent A was reduced from 90% to 70% within 6 min followed by isocratic elution for 4 min. The fragmentation of [M + NH4]+ adduct ions to [M-fatty acid anion]+ ions was measured by multiple reaction monitoring. The ion spray voltage was set to 5500 V, the curtain gas to 30 psi (QTRAP 5500) or 40 psi (QTRAP 6500+), the collision gas to low, and the heated capillary temperature to 400 °C. The sheath gas pressure was set to 60 psi and the auxiliary gas pressure was set to 70 psi. The declustering potential was set to 120 V, the entrance potential to 10 V, the collision energy to 35 eV, and the collision cell exit potential to 26 V.
The instruments were either operated with Analyst 1.6.2 (QTRAP 5500, Sciex) or Analyst 1.7.1 (QTRAP 6500+, Sciex).
DOI: 10.7483/opendata.cms.6dfn.rihp
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-2400_MY-60_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.34816/efa.archives.509433
title: Delphes-N336-023-Colonne d'Acanthe, base

description: Référence : N336-023<br />Publication(s) : FD IV, 8.2, p. 554, fig. 22<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.48323/61r67-mjv24
title: Concentration of α-amplexichromanol (27a) in lung and plasma of mice with ovalbumin induced asthma

description: Neukirch, K. et al. Exploration of Long-Chain Vitamin E Metabolites for the Discovery of a Highly Potent, Orally Effective, and Metabolically Stable 5-LOX Inhibitor that Limits Inflammation. J Med Chem 64, 11496-11526 (2021). https://doi.org/10.1021/acs.jmedchem.1c00806
BALB/c mice received 27a (p.o.) or vehicle (0.5% carboxymethylcellulose in 10% Tween 20; 0.5 mL) on days 0 and 7, 1 h (p.o) before being sensitized to ovalbumin (100 μg adsorbed to 3.3 mg of aluminum hydroxide gel, s.c., Sigma-Aldrich). Mice were sacrificed on days 1, 3, 8, 10, or 21 to collect lung and plasma. Compound 27a was analyzed in plasma and lung homogenates by UPLC-MS/MS as described in Neukirch et al., 2021. Lung tissue (100 mg/mL) was homogenized in PBS pH 7.4 at 4°C for 1−2 min using an Omni tissue homogenizer (Omni, Kennesaw, GA).
DOI: 10.48323/v9qyw-9nj08
title: Analysis of the phosphatidylcholine fatty acid composition in α-T-13′-COOH-treated macrophages

description: RAW264.7 cells were incubated with vehicle (DMSO, 'w/o') or 0.5 or 5.0 µM α-T-13′-COOH for 24 h. The fatty acid distribution of phosphatidylcholine was then analyzed by UPLC-MS/MS.
Raw analyst files (.wiff and .wiff.scan) of the UPLC-MS/MS results were uploaded, together with an Excel file for the sample list.
The methods and results were published in Liao et al., Int J Mol Sci, 2023 May 25;24(11):9229. doi: 10.3390/ijms24119229.
Lipids were extracted from RAW264.7 cell pellets by the successive addition of methanol, PBS (pH 7.4), chloroform, and saline (final ratio: 34:14:35:17). After the evaporation of the organic solvent, the remaining lipid fraction was dissolved in methanol, stored at −20 °C, and analyzed by UPLC-MS/MS. Internal standards: 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidyl-ethanolamine (DMPE).
Phospholipids (PC, PE, PI, PS, PG) were separated on an Acquity UPLC BEH C8 column (130 Å, 1.7 μm, 2.1 × 100 mm; Waters, Milford, MA, USA) using an Acquity UPLC system (Waters), which was coupled to a QTRAP 5500 mass spectrometer (Sciex, Framingham, MA, USA) equipped with a Turbo V Ion Source and an electrospray ionization probe. Chromatographic separation was performed at a flow rate of 0.75 mL/min and at a column temperature of 45 °C. The mobile phase was composed of eluent A (acetonitrile/water, 95/5, with 2 mM ammonium acetate) and eluent B (water/acetonitrile, 90/10, with 2 mM ammonium acetate). The gradient was ramped from 70% to 80% A within 5 min and to 100% A within 2 min, followed by isocratic elution for another 2 min. Eluted phospholipids were detected upon the fragmentation of parental ions (PC: [M+OAc]−, all other phospholipids: [M-H]−) to fatty acid anions derived from sn-1 and sn-2 positions by multiple reaction monitoring using a QTRAP 5500 mass spectrometer. The ion spray voltage was set to −4500 V, the curtain gas to 30 psi, the collision gas to medium, and the heated capillary temperature to either 350 °C (PC), 500 °C (PI), 550 °C (PS, PG), or 650 °C (PE). The sheath gas pressure was set to 45 (PS) or 55 psi (PC, PE, PI, PG) and the auxiliary gas pressure was set to either to 75 psi (PC, PE, PI, PG) or 80 psi (PS). The declustering potential was set to −40 V (PS), −44 V (PC), −45 V (PG), or −50 V (PE, PI), the entrance potential to −10 eV (PC; PE, PI, PS, PG), the collision energy to −38 eV (PE), −46 eV (PC), −52 eV (PG), −56 eV (PS), or −62 eV (PI), and the collision cell exit potential to −11 V (PC, PI), −12 V (PE), −18 V (PG), or −20 V (PS).
The instruments were either operated with Analyst 1.6.2 (QTRAP 5500, Sciex) or Analyst 1.7.1 (QTRAP 6500+, Sciex).
DOI: 10.34816/efa.archives.509432
title: Delphes-N336-022-Colonne d'Acanthe, base

description: Référence : N336-022<br />Publication(s) : FD IV, 8.2, p. 554, fig. 22<br />Période(s) : Classique; Antiquité<br />Domaine : Architecture<br />
DOI: 10.48323/nxvby-k9x34
title: Effect of curcuminoids on cell-free microsomal prostaglandin E synthase-1 activity

description: Human mPGES-1 was pre-treated with DMSO or curcuminoids, the formation of PGE2 was initiated by the addition of PGH2 and the mPGES-1 product PGE2 was analyzed by RP-UV-HPLC.
Raw data including the absolute values (ng) and normalized data (% of control) of all individual experiments was uploaded.
The methods and results were published in Rao et al., Biochem Pharmacol. 2022 Sep:203:115202. doi: 10.1016/j.bcp.2022.115202 
mPGES-1 activity was measured in microsomal membranes of IL-1β-treated human lung adenocarcinoma epithelial A549 cells. A549 cells were incubated with IL-1β (2 ng/mL, Peprotech, Hamburg, Germany) in DMEM/high glucose (4.5 g/L) medium plus FCS (10 %) and penicillin/streptomycin (100 U/mL and 100 μg/mL; GE Healthcare, Freiburg, Germany) at 37 °C and 5 % CO2 for 48 h. Cell pellets were harvested and snap-frozen in liquid nitrogen. After resuspension and incubation in ice-cold homogenization buffer [potassium phosphate buffer (0.1 M, pH 7.4), phenylmethylsulphonyl fluoride (1 mM, Cayman Chemical), soybean trypsin inhibitor (60 μg/mL, Cayman Chemical), leupeptin (1 μg/mL, Cayman Chemical), glutathione (2.5 mM, Cayman Chemical) and sucrose (250 mM, Cayman Chemical)] for 15 min on ice, cells were sonicated (3 times, 20 s, each, at 4 °C). Differential centrifugation (first 10,000 × g, 10 min; then 174,000 × g, 60 min; at 4 °C) yielded the microsomal membrane fraction, which was resuspended in homogenization buffer and diluted in potassium phosphate buffer (0.1 M, pH 7.4) plus glutathione (2.5 mM, Cayman Chemical). 
To determine mPGES-1 activity, membranes (containing 2.5–5 μg total protein in 50 μL homogenization buffer) were pre-treated with vehicle (DMSO), test compounds or the mPGES-1 inhibitor MK-886 (10 μM, Cayman Chemical) for 15 min at 4 °C. The formation of PGE2 was initiated by the addition of PGH2 [20 μM in 50 µL potassium phosphate buffer (0.1 M, pH 7.4) containing phenylmethylsulphonyl fluoride (1 mM, Cayman Chemical), soybean trypsin inhibitor (60 μg/mL, Cayman Chemical), leupeptin (1 μg/mL, Cayman Chemical), glutathione (2.5 mM, Cayman Chemical)]. After 1 min incubation at 4 °C, the reaction was terminated with an equal volume of stop solution containing FeCl2 (40 mM) and citric acid (80 mM) as well as the internal standard 11β-PGE2 (1 nmol, Cayman Chemical). The mPGES-1 product PGE2 was extracted by solid phase extraction using Sep-Pak C18 35 cc Vac Cartridges (Waters), separated on Nova-Pak C18 Radial-Pak Column (4 μm, 5 × 100 mm, Waters) under isocratic conditions (30 % acetonitrile, 70 % water, 0.007 % trifluoroacetic acid) at a flow rate of 1 mL/min and detected at 195 nm. The mPGES-1 reference inhibitor MK-886 (10 μM, Cayman Chemical) inhibited PGE2 formation by 86.4 ± 0.5 %.