DOI: 10.5061/dryad.k7d00
title: Data from: Inbreeding reveals mode of past selection on male reproductive characters in Drosophila melanogaster

description: Directional dominance is a prerequisite of inbreeding depression.
 Directionality arises when selection drives alleles that increase fitness
 to fixation and eliminates dominant deleterious alleles, while deleterious
 recessives are hidden from it and maintained at low frequencies. Traits
 under directional selection (i.e., fitness traits) are expected to show
 directional dominance and therefore an increased susceptibility to
 inbreeding depression. In contrast, traits under stabilizing selection or
 weakly linked to fitness are predicted to exhibit little-to-no inbreeding
 depression. Here, we quantify the extent of inbreeding depression in a
 range of male reproductive characters and then infer the mode of past
 selection on them. The use of transgenic populations of Drosophila
 melanogaster with red or green fluorescent-tagged sperm heads permitted in
 vivo discrimination of sperm from competing males and quantification of
 characteristics of ejaculate composition, performance, and fate. We found
 that male attractiveness (mating latency) and competitive fertilization
 success (P2) both show some inbreeding depression, suggesting they may
 have been under directional selection, whereas sperm length showed no
 inbreeding depression suggesting a history of stabilizing selection.
 However, despite having measured several sperm quality and quantity
 traits, our data did not allow us to discern the mechanism underlying the
 lowered competitive fertilization success of inbred (f = 0.50) males.
description: P2
 dataData for P2 analysis,
 and for offspring production before and after remating in the P2
 experiment. Column abbreviations: id = female id, trt = treatment: aob =
 outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f =
 0.25), Fthorax = female thorax lentgh, M2thorax = 2nd male thorax lentgh,
 P2day = day to which P2 value refers to, gl = number of first male's
 offspring, ngl = number of second male's offspring, totpriroprog=
 number of offspring produced before second mating, P2 = P2 (proportion),
 totremoffspring = number of offspring produced after second mating, asp2 =
 acrsin sqrt transformed P2 valuedatap2.txtcopulation-duration-with-virginCopulation durations with virgin females in single-mating-productivity experiment. Column abbreviations: trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Femaleth = female thorax lentgh, Maleth = male thorax lentgh, Matlat = mating latency (min), copdur = copulation durationCopulation duration with non-virginCopulation durations with non-virgins in the P2 expriment. Column abbreviations: id = female id, trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25),line = the line male originated from, Fthorax = female thorax lentgh, M2thorax = 2nd male thorax lentgh, remday = remating day, copdur2 = duration of the second copulation2ndcopulationduration.txtmating latency (male attractiveness)Mating latencies with virgins in the single-mating-productivity experiment. Column abbreviations: trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Femaleth = female thorax lentgh, Maleth = male thorax lentgh, Matlat = mating latency (min), copdur = copulation durationmating-latency.txtremating dayRemating days in the P2 experiment. Column abbreviations: id = female id, trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25),line = the line male originated from, Fthorax = female thorax lentgh, M2thorax = 2nd male thorax lentgh, remday = remating day, copdur2 = duration of the second copulationrematingday.txtsingle-mating-productivityOffspring production during ten days after single mating. Column abbreviations: trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Maleth = male thorax lentgh, Femaleth = female thorax lentgh, Mfamoforig = Male line of origin (within treatment), progeny = number of eclosed offspring, fday = day as a factorspermcounts 60min after 2nd copulation startedSperm counts in 60 min ASM treatment. Column abbreviations: ID = female id, trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Fthorax = female thorax lentgh, M2thorax = 2nd male thorax lentgh, bursagr = 1st male sperm in bursa, bursared = 2nd male sperm in bursa, prSRgr = 1st male sperm in proximal SR (seminal receptacle), prSRred = 2nd male sperm in proximal SR, distSRgr = 1st male sperm in distal SR, distSRgr = 2nd male sperm in distal SR, SPT1gr = 1st male sperm in spermatheca (SPT) 1, SPT1red = 2nd male sperm in spermatheca 1, SPT2gr = 1st male sperm in spermatheca 2, SPT2red = 2nd male sperm in spermatheca 2, duct1gr = 1st male sperm in spermathecal duct 1, duct1red = 2nd male sperm in spermathecal duct 1, duct2gr = 1st male sperm in spermathecal duct 2, duct2red = 2nd male sperm in spermathecal duct 2, SRtotred = 2nd male sperm in SR, TOTred = total number of 2nd male sperm, SPTtotred = total number of 2nd male sperm in SPT, STOREDred =total number of 2nd male sperm in SR and SPT, remday = remating day, copdur = copulation duration, totgr = total number of first male spermspermcounts60min.txtsperm counts 5h after 2nd copulation startedSperm counts from 5 h ASM treatment. Column abbreviations: ID = female id, trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Fthorax = female thorax lentgh, M2thorax = 2nd male thorax lentgh, bursagr = 1st male sperm in bursa, bursared = 2nd male sperm in bursa, prSRgr = 1st male sperm in proximal SR (seminal receptacle), prSRred = 2nd male sperm in proximal SR, distSRgr = 1st male sperm in distal SR, distSRgr = 2nd male sperm in distal SR, SPT1gr = 1st male sperm in spermatheca (SPT) 1, SPT1red = 2nd male sperm in spermatheca 1, SPT2gr = 1st male sperm in spermatheca 2, SPT2red = 2nd male sperm in spermatheca 2, duct1gr = 1st male sperm in spermathecal duct 1, duct1red = 2nd male sperm in spermathecal duct 1, duct2gr = 1st male sperm in spermathecal duct 2, duct2red = 2nd male sperm in spermathecal duct 2, SRtotred = 2nd male sperm in SR, TOTred = total number of 2nd male sperm, SPTtotred = total number of 2nd male sperm in SPT, STOREDred =total number of 2nd male sperm in SR and SPT, remday = remating day, copdur = copulation duration, totgr = total number of first male sperm, S2SR = proportion of second male sperm in SR, asS2= acrsin sqrt transformed S2 valuespermcounts5h.txtsperm counts 6 days after 2nd copulationSperm counts from the 6 day ASM treatment. Column abbreviations: ID = female id, trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Fthorax = female thorax lentgh, M2thorax = 2nd male thorax lentgh, bursagr = 1st male sperm in bursa, bursared = 2nd male sperm in bursa, prSRgr = 1st male sperm in proximal SR (seminal receptacle), prSRred = 2nd male sperm in proximal SR, distSRgr = 1st male sperm in distal SR, distSRgr = 2nd male sperm in distal SR, SPT1gr = 1st male sperm in spermatheca (SPT) 1, SPT1red = 2nd male sperm in spermatheca 1, SPT2gr = 1st male sperm in spermatheca 2, SPT2red = 2nd male sperm in spermatheca 2, duct1gr = 1st male sperm in spermathecal duct 1, duct1red = 2nd male sperm in spermathecal duct 1, duct2gr = 1st male sperm in spermathecal duct 2, duct2red = 2nd male sperm in spermathecal duct 2, SRtotred = 2nd male sperm in SR, TOTred = total number of 2nd male sperm, SPTtotred = total number of 2nd male sperm in SPT, STOREDred =total number of 2nd male sperm in SR and SPT, remday = remating day, copdur = copulation duration, totgr = total number of first male sperm, logGR = log transformed 1st male sperm, S2 = proportion of second male sperm in SR, asS2= acrsin sqrt transformed S2 value, S2spt = proportion of second male sperm in SPTspermcounts6days.txtsperm length dataSperm lengths in different inbreeding levels. Column abbreviations: trt = treatment: ob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25),male = male id, line = the line male originated from, (within treatment), Thorax = male thorax lentgh, Slentgh = sperm lentgh (mm)spermlentgh.txtsperm velocitySperm velocity in different male inbreeding levels. Column abbreviations: id = male id, trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Track = individual sperm followed for ten frames, velocity = sperm velocity, spermcount = number of sperm in seminal receptacle, Fthorax = female thorax lentgh, Mthorax = male thorax lentgh, correctedvelocity = velocity in microm/s, locvel = log-transformed correctedvelocityspermvelocity.txtegg-to-adut viabilityEgg-to-adult viability on days 1, 3 and 5 in the single-mating-productivity experiment. Column abbreviations: trt = treatment: aob = outbred (f =0), ib = highly inbred (f = 0.5), sib = moderately inbred (f = 0.25), Maleth = male thorax lentgh, Femaleth = female thorax lentgh, viab = viability, fday = day as a factorviability.txt