DOI: 10.5061/dryad.559907q
title: Data from: Plasma metabolomic biomarkers accurately classify acute mild traumatic brain injury from controls

description: Past and recent attempts at devising objective biomarkers for traumatic
 brain injury (TBI) in both blood and cerebrospinal fluid have focused on
 abundance measures of time-dependent proteins. Similar independent
 determinants would be most welcome in diagnosing the most common form of
 TBI, mild TBI (mTBI), which remains difficult to define and confirm based
 solely on clinical criteria. There are currently no consensus diagnostic
 measures that objectively define individuals as having sustained an acute
 mTBI. Plasma metabolomic analyses have recently evolved to offer an
 alternative to proteomic analyses, offering an orthogonal diagnostic
 measure to what is currently available. The purpose of this study was to
 determine whether a developed set of metabolomic biomarkers is able to
 objectively classify college athletes sustaining mTBI from non-injured
 teammates, within 6 hours of trauma and whether such a biomarker panel
 could be effectively applied to an independent cohort of TBI and control
 subjects. A 6-metabolite panel was developed from biomarkers that had
 their identities confirmed using tandem mass spectrometry (MS/MS) in our
 Athlete cohort. These biomarkers were defined at ≤6 hours following mTBI
 and objectively classified mTBI athletes from teammate controls, and
 provided similar classification of these groups at the 2, 3, and 7 days
 post-mTBI. The same 6-metabolite panel, when applied to a separate,
 independent cohort provided statistically similar results despite major
 differences between the two cohorts. Our confirmed plasma biomarker panel
 objectively classifies acute mTBI cases from controls within 6 hours of
 injury in our two independent cohorts. While encouraged by our initial
 results, we expect future studies to expand on these initial observations.
description: READ
 MEIntroduction to the
 uploaded files.PLOS ONE Supporting Info
 ArchiveManuscript Supporting
 information, including S Figs, S Files, and S Tables from the
 manuscript.MSFmTBIExcelDataExcel files of Metabolomic XCMS data and demographic information regarding the Athlete Cohort and External Cohort used in the manuscript.Dataset_Sample_ReferenceAdditional Reference file for both the Athlete Cohort and the External Cohort used in the manuscript.Athlete NEG RAW1RAW data for the Athlete Cohort from the Negative ESI mode. Part 1 of 4.Athlete NEG RAW2RAW data for the Athlete Cohort from the Negative ESI mode. Part 2 of 4.Athlete NEG RAW3RAW data for the Athlete Cohort from the Negative ESI mode. Part 3 of 4.Athlete NEG RAW4RAW data for the Athlete Cohort from the Negative ESI mode. Part 4 of 4.Athlete POS RAW1RAW data for the Athlete Cohort from the Positive ESI mode. Part 1 of 4.Athlete POS RAW2RAW data for the Athlete Cohort from the Positive ESI mode. Part 2 of 4.Athlete POS RAW3RAW data for the Athlete Cohort from the Positive ESI mode. Part 3 of 4.Athlete POS RAW4RAW data for the Athlete Cohort from the Positive ESI mode. Part 4 of 4.External.TBI_Metabolomics_RAW_DATARAW data files for the External Cohort from both the Negative and Positive ESI modes.External.TBI_Metabolomics_RAW_Data_files.zip