cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0nc607j
title: CIL:9064, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
description: The events of meiosis I in a living insect spermatocyte beginning at metaphase with bivalents at the spindle equator, followed by anaphase onset, chromosome segregation and the start of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 105 min by a QImaging Retigo EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).
DOI: 10.6075/j06m35vx
title: CIL:9063, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: The events of meiosis I in a living insect spermatocyte beginning at metaphase with meiotic bivalents at the spindle equator followed by anaphase , segregation of bivalents to the spindle poles and the near completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 90 min by a QImaging Retiga EXi CCD camera. A movie prepared from this image stack is included in the grouped set.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
DOI: 10.6075/j08051xb
title: CIL:908, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 40,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:907.
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
DOI: 10.6075/j0gf0skd
title: CIL:9071, Mus musculus. In Cell Image Library

description: Preparation: unfixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
description: Murine adipocytes differentiated from 3T3-L1 cells showing intracellular trafficking of GFP-GLUT4 vesicles. GLUT 4 (Glucose Transporter Type 4) is a glucose transport protein that is responsible for insulin-regulated glucose translocation into the cell.

Fluorescence TIRFM Image. Leica TIRFM Objective used: 100X 1.46 NA. Images captured with a Hamamatsu EMCCD camera.
DOI: 10.6075/j0p55ms1
title: CIL:907, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 40,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:908
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
DOI: 10.6075/j00g3j4t
title: CIL:9069, Homo sapiens, osteosarcoma. In Cell Image Library

description: Saos2 cell line immunostained with Tom20 (mitochondrial outer membrane protein, green) and DLP1 (dynamin like protein, red) antibody. Cell population synchronized at G1-S transition of the cell cycle. This Saos2 cell line was serum starved (0.1%) for 3 days and released in FCCP (10 mM) ) for 16 hours. The image is a single 3 micron confocal section collected on a Zeiss LSM 510 with the following parameters: Green: Ex: 488 nm laser, Em: Band Pass 500-550nm, Red: 543 nm laser, Em: Long pass 560 nm.
description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data; color combine
Data qualification: Raw
DOI: 10.6075/j03f4nww
title: CIL:914, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:1316.
DOI: 10.34810/data1216
title: Methodological Videography of Bird Butchery: Replicating Early Human Techniques in Experimental Archaeology

description: This dataset encompasses the presentation of five raw video recordings, wherein Anna Rufà and Mariana Nabais engage in the processing of five bird specimens. These activities are designed to replicate the potential butchery movements executed by early humans. The selected species for this experimental endeavour include Corvus corone (Carrion Crow), Columba palumbus (Wood Pigeon), and Streptopelia decaocto (Collared Dove).
&lt;br&gt;
The avian specimens were obtained from the Wildlife Ecology, Rehabilitation, and Surveillance Centre (CERVAS), affiliated with the ICNF Serra da Estrela Natural Park, situated in Gouveia, Portugal. These specimens succumbed to natural causes within the confines of the rehabilitation centre, were subjected to veterinary examinations, and subsequently preserved through freezing for the purposes of scientific inquiry post-mortem. The specimens were thawed 24 hours in advance of the experimental procedures to ensure their resemblance to their natural, raw state in terms of texture and appearance.
&lt;br&gt;
To elucidate the array of potential dietary practices among early humans, two birds (Birds 1 and 2) were processed in their raw state, while the remaining three (Birds 3, 4, and 5) underwent processing post-roasting.
&lt;br&gt;
Bird 1, a Streptopelia decaocto, was processed in its raw form by Anna Rufà on November 6, 2023. Similarly, Bird 2, a Corvus corone, was also processed in its raw state by Mariana Nabais on the same date. Birds 3 and 4, Streptopelia decaocto and Corvus corone respectively, were roasted prior to processing by Anna Rufà on November 8, 2023. Lastly, Bird 5, a Columba palumbus, was roasted and then processed by Mariana Nabais on November 8, 2023. This methodological approach aims to provide a comprehensive understanding of the diverse potential consumption practices of Neanderthals, as reflected through the experimental processing of these avian specimens.
&lt;br&gt;
The provided recordings are unedited and were captured using a Nikon D5300 camera alongside an iPhone 11 and an Android Pixel 4a. These videos serve as a preliminary archival resource, preserving the methodology and specific procedural steps for future scholarly reference and the facilitation of experimental reproducibility.
DOI: 10.6075/j0bc3xsz
title: CIL:911, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:912.
DOI: 10.5281/zenodo.17388350
title: Observations of waves, currents, and wind forcing at ASIT (Martha's Vineyard, MA, USA), Fall-Winter of 2019-2020

description: This dataset contains observations of waves, currents, and wind forcing collected at the Air-Sea Interaction Tower (ASIT) south of Martha's Vineyard, MA, USA in late 2019 - early 2020 by a collaborative group (PIs Alejandro Cifuentes-Lorenzen, UConn; Chris Zappa, LDEO-Columbia University; Jim Edson, WHOI). Short wave observations were made via polarimetric camera by Chris Zappa, Nathan Laxague, and collaborators. The files "ASIT2019_wave_spectra_stats_timeseries_empirical_gain.nc" and "ASIT2019_supporting_environmental_observations.nc" are sufficient for the purpose of describing the conditions observed during this period. The inclusion of the other files are justified in the associated code repository (https://github.com/unh-cassll/E-PSS_paper). Codes in that repository also allow one to compute the long wave directional spectra ('ASIT2019_EPSS_directional_spectra.nc') and the short wave slope statistics ('slope_statistics_dataset.nc'). The distinctions between "no_gain", "lab_gain", and "empirical_gain" are outlined in the manuscript contained within the GitHub repository. In short, they represent three distinct approaches to correcting the measured polarization state of light reflected off the sea surface for the purpose of inferring surface wave structure.
DOI: 10.6075/j01c1vzt
title: CIL:9097, Homo sapiens, epithelial cell, permanent cell line cell. In Cell Image Library

description: Hela cells transiently transfected with EGFP-GL-GPI show the organization of the membrane thru the distribution of GPI anchored protein. 
This image is the z stack that accompanies the related maximum z projection image. 
The images were collected on a Zeiss spinning disk confocal microscope using a 63 X 1.4 NA oil immersion objective. 56 z slices at 0.27 micron intervals were collected with a 450 ms exposure using a 488 ht.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
DOI: 10.6075/j0cz36c2
title: CIL:909, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:915.
DOI: 10.6075/j0gm86cf
title: CIL:9059, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
description: The events of meiosis II in a living insect spermatocyte beginning at the completion of prophase II through the comletion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 3 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. A movie prepared from this stack of images is included in this grouped set.
DOI: 10.6075/j0h41qgt
title: CIL:9057, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: The events of meiosis I in a living insect spermatocyte beginning at diakinesis through telophase to the near completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 4 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. A movie prepared from this stack of images is included in this grouped set of images.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm t
DOI: 10.6075/j0cf9p58
title: CIL:9066, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
description: The events of meiosis I in a living insect spermatocyte beginning at metaphase I with bivalents at the spindle equator followed by anaphase, segregation of half-bivalents diakinesis and commencement of cytokinesis. Time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 105 min by a QImaging Retigo EXi CCD camera. Raw images were processed using 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).
DOI: 10.6075/j0td9wf1
title: CIL:9056, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: The events of meiosis I in a living insect spermatocyte beginning at diakinesis through telophase to the near completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 4 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. Raw images were processed using 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 4-frame algorithm
DOI: 10.6075/j04f1pzc
title: CIL:904, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:903
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
DOI: 10.6075/j01n80f5
title: CIL:903, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:904
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
DOI: 10.6075/j0q23zh3
title: CIL:902, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:913.