cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.482
title: Solid phase extraction of mercury from seawater using sulfur powder chemically modified with N-(2-chlorobenzoyl)-N’-phenylthiocarbamide and analysis by atomic fluorescence spectrometry

description: A simple method has been tested and validated for the preconcentration of mercury from seawater base on sulfur chemically modified with N-(2-chloro benzoyl)-N'-phenylthiocarbamide. This technique offers an advantage of avoiding matrix effect from the saline content in water sample during analysis. Various conditions such as pH solution, ligand quantity, eluent type, flow rate, sample volume and sorption capacity have been studied in detail. The calibration graph was linear in the range 0-20 ng L-1 of mercury with the detection limit of 3.18 ng L-1. The relative standard deviation was found to be 1.024%. The procedure was validated by analysis of certified reference material, Double deionized water and synthetic seawater.
DOI: 10.14457/cu.the.2015.483
title: STOPPING CRITERIA FOR REGRESSION TESTING IN GUI APPLICATION USING FAILURE INTENSITY AND FAILURE RELIABILITY

description: This research proposes some criteria for GUI regression testing to determine the appropriate time to stop without wasting too much testing cost. This is essential for all software upgrades that can be released in a reasonably short time, yet still guarantees the product quality. One difficulty to achieve such a target depends on the sequence of test cases being input. The order of the input test case input sequence affects the number of failures found. As such, a test-stoppage model is proposed by determining factors that affect software reliability and the expected cost of continuing test. The procedure prioritizes the order of test cases into different sequences for the regression test input. When a failure is found, it is immediately edited before the test resumes. The test terminates when the failure intensity is within the predetermined threshold and the expected cost does not exceed the allotted budget limit. Performance of the proposed criteria encompasses three measures, namely, failure intensity, cost of testing and editing, and reliability. Failure intensity is a function of faults and fault detection rate. The costs are function of time spent on fixing errors. The reliability function incorporates Weibull distribution to better reflect the test data. The proposed model is tested using real GUI applications as test data. Performance shows satisfactory results on stopping criteria.
DOI: 10.14457/cu.the.2015.484
title: PROTEOMICS OF RED KWAO KRUA Butea superba Roxb. TUBERS

description: Red Kwao Krua (Butea superba Roxb.) is a Thai traditional medicinal plants used in alternative medicines for anti-aging and increased sex vigor in male. Seasonal changes especially changes in temperature and amount of rainfall are major influencing factors which results in induction of protein biosynthesis in response to plant stresses, leading to changes in protein relative abundance in metabolic pathways of plant cells. Proteomics was applied in this study using the two-dimensional polyacrylamide gel electrophoresis for protein identification coupled with mass spectrometry, to study the variations in proteome compositions of B. superba tubers and leaves collected during rainy season, winter and summer. The protein separation results demonstrated the identified 224 protein spots in tubers and 112 protein spots in leaves which were classified to be the same amount of 12 functional protein groups. The 45 and 12 identified proteins in tubers and leaves were involved in multiple metabolic pathways in different seasons. The analysis of the protein spot intensities indicated that the differential amount of expressed proteins might be induced from the increased temperature and water shortage stresses occurred during summer and the decreased temperature and water shortage stresses occurred during winter might induce the differential abundance levels. It might result in changes in the physiological status within plant tubers and leaves, including phonotypic changes related to plant survival. The results might help the molecular basis understanding in tuber and leaf proteomes. In additional, the distinct of some proteins could potentially be used as protein markers to indicate the responses to the physical factors occurred during seasonal changes in other plants. It might also applied for setting of harvest of B. superba tubers and leaves for manufacturing of products.
DOI: 10.14457/cu.the.2015.485
title: SYNTHESIS AND APPLICATIONS OF MORPHOLOGICALLY CONTROLLED SILVER MICRO/NANOSTRUCTURES

description: In this work, three morphologically controlled synthesis protocols of silver micro/nanostructures using chemical approach were developed. The detailed growth mechanisms were extensively studied. The first work was the morphology controlled synthesis of silver micro/nanoplates under an etching environment using hydrogen peroxide (H2O2) as a reducing agent. The results revealed that chloride ions (Cl-) were essential for creating etching environment capable of selective dissolution of single crystal and multiply twinned crystal, while leaving plate structures unaffected. The second work was the morphology controlled synthesis of silver chloride (AgCl) microstructures via a precipitation of silver ammine complex ([Ag(NH3)2]+) by an addition of Cl-. The growth mechanism suggested that the eight-pod family grew from the cubic seeds in Cl--rich environment while the six-pod family grew from an octahedral seeds in an NH4OH-rich environment. The third work was the morphology controlled synthesis of nanoporous silver microstructures via galvanic replacement of AgCl microstructures. The concerting reaction between oxidative etching of nanoporous silver microstructures and re-deposition of Ag atoms converts large silver structures to small structures. The research findings enabled a development of a large scale silver recovery protocol from industry and laboratory wastes by converting AgCl precipitates into highly pure silver microstructures (&gt;99.99%). The recovered products could be directly employed as a raw material for silver jewelry applications.
DOI: 10.14457/cu.the.2015.486
title: CLUSTER OF CARBON NANOSPHERES AS DRUG CARRIER

description: Cluster of carbon nanospheres, or the so called oxidized carbon nanospheres (OCSs) were successfully synthesized. The use of OCSs as drug carrier was, then, investigated by comparison to the different shaped oxidized carbon particulates, oxidized carbon nanotubes (OCTs) and oxidized graphene sheets (OGShs). The result reveals that OCSs possessed highest ability to associate with lipid bilayer membrane and highest level of penetration into cell-sized liposomes, whereas OCTs showed the moderate adhesion with lipid membrane without liposome penetration and OGShs exhibited no adhesion to the membrane. Regarding lipid composition of the membrane, the OCSs more preferably adhere on the liquid-disordered (Ld) membrane (composes of high fluidity lipid molecules) as compared to the liquid-ordered (Lo) membrane (composes of less fluid lipid molecules), owning to the more favorable free energy of membrane curvature. OCSs could effectively deliver cargo, here curcumin was used as model cargo, into A549 lung cancer cells and allowed accumulation of curcumin in both cytoplasm and nucleus of the cells. OCTs could induce only moderate uptake of curcumin into the cytosol of the cells, whereas OGShs were rarely internalized into cells. Surface modification of oxidized carbon particulates by mPEG decreases their ability to associate with lipid bilayer membrane. In addition, the OCSs could deliver both hydrophobic and hydrophilic anti-cancer drugs into cancer cells, A549 and A549RT-eto. The results reveal that delivering of paclitaxel, which is a hydrophobic drug, by OCSs showed anti-cancer activity at the same level as use of the free drug in DMSO. This could be implied that delivery by OCSs improve cellular uptake ability of the water insoluble drug and avoid use of organic solvent.
DOI: 10.14457/cu.the.2015.487
title: Blood cell morphology and hematological parameters of rice field frog Hoplobatrachus rugulosus (Wiegmann, 1834)

description: The rice field frog Hoplobatrachus rugulosus, a common anuran species found in wetlands throughout Thailand, is well known as an economically important farm animal. Hematological technique is a basic method to determine health of animals. Even though the data on hematology is crucial for manipulation and conservation of this frog, they are still very limited. Our research group tries to develop health assessment criteria based on hematological parameters of the frog. Therefore, hematological parameters including blood cell morphology and morphometry of wild caught H. rugulosus and hematological parameters with the normal reference values of farm raised H. rugulosus were examined in this study. The adult wild frogs were collected from natural habitat at Nan Province in wet season, 2014. The farm raised frogs including adult, juvenile and sicked frogs were collected from farm in Chiang Mai Province in 3 seasons during 2014. Blood samples were subjected for erythrocyte and leukocyte count by hemocytometer, packed cell volume (PCV) estimation, differential leukocyte count from Giemsa-stained blood smears and total protein quantification by Bradford assay. The results showed the morphological characteristics of wild H. rugulosus blood cells including erythrocytes, immature erythrocytes, thrombocytes, lymphocytes, monocytes, neutrophils, basophils and eosinophils. In wild caught frogs, the mean of PCV, number of lymphocytes and neutrophils showed significant sex-related difference. The hematological parameters of farm raised adult H. rugulosus that showed significant sex-related differences include erythrocyte count, PCV, plasma total protein, differential lymphocyte count, differential monocyte count, and differential eosinophil count. The hematological parameters of farm raised juvenile frogs that showed significant sex-related differences include PCV, plasma total protein, differential lymphocyte count, differential monocyte count, differential neutrophil count and differential eosinophil count. Most of hematological parameters of farm raised adult and juvenile frogs also showed seasonal variation except the differential basophil count. The normal reference values that have been established and validated to be sensitively used for monitoring of health status of adult farm raised H. rugulosus include erythrocyte count (for female in wet season), plasma total protein (for both sexes in cool dry season) and differential lymphocyte count (for both sexes in wet season). The hematological parameters and the normal reference values presented in this study could be regarded as the first report and a crucial baseline data for conservation and management of wild and farm raised H. rugulosus in Thailand.
DOI: 10.14457/cu.the.2015.488
title: Sound characteristics of captive Irrawaddy dolphin Orcaella brevirostris

description: Even though there have been several studies on vocal repertoires and behaviours of Irrawaddy dolphins (Orcaella brevirostris) in recent decades, important pieces of information are still missing, especially the sounds produced in captivity where environmental differences can affect their physical and acoustic behaviours. Over 40 hours of observations in March and November 2014 with almost 7,000 vocalization samples, it was determined that the captive Irrawaddy dolphins produced various types of sound including two types of clicks (n = 3,898), five types of pulsed sounds (n = 2,807), and nine types of whistles (n = 44). This work also describes three newly discovered pulsed sounds and all nine whistles types in this species. Clicks were observed most frequently during feeding and swimming in navigational contexts. Pulsed sounds were mainly used in social or emotional contexts associated with various behaviours, even aggression, sexual arousal, and tourist-encounter. Whistles, on the other hand, were scarcely heard which might imply that the communication was not necessary. Whistling behaviour was contradictory to those reported for other wild delphinids which were potentially caused by habituation in captivity. Overall vocalizations, which were concurrent with activity levels, were recorded at a significantly higher rate (p = 0.000009) when tourists were present during “swim-with-dolphin” programs. This is the first study to correlate Irrawaddy dolphin’s sounds and behaviours in captivity and represents the first time that all three sound categories have been recorded for this species in captivity.
DOI: 10.14457/cu.the.2015.489
title: Development of microfluidic analytical device for determination of heavy metals

description: This research aimed to develop a microfluidic analytical device for determination of heavy metals, which can be divided into 3 parts. The first part is the development of microfluidic paper-based colorimetric device using thiosulfate catalytic etching of silver nanoplates for determination of copper. This device offers high sensitivity and selectivity for determination of copper over other metal ions. The color change can be monitored by the naked eye. The limit of detection was found to be 0.3 ng mL-1 and the relevant calibration curves was linear in the range of 0.5 - 200.0 ng mL-1 by ImageJ analysis. The second part is simultaneous determination of lead, cadmium and copper using microfluidic paper-based analytical device with dual electrochemical and colorimetric detection. Electrochemical detection was applied for determination of lead and cadmium using a bismuth film modified boron-doped diamond electrodes (Bi-BDDE). For the copper assay, the concentration of copper was measured by colorimetric detection based on the catalytic etching of silver nanoplates by thiosulfate. Under optimized conditions, the linear range obtained was found to be 0.5 - 70 ng mL-1 for lead and cadmium, 10 - 350 ng mL-1 for copper, and the detection limits were 0.1 ng mL-1 for lead and cadmium, and 5.0 ng mL-1 for copper. The final part is development of analytical method for the determination of heavy metal using a nafion/ionic liquid/graphene composite modified screen-printed carbon electrode. The composite-modified electrode was fabricated and evaluated for the highly sensitive simultaneous determination of zinc, cadmium and lead, which the detection limits of the simultaneous analyses were 0.09 ng mL-1, 0.06 ng mL-1 and 0.08 ng mL-1, respectively. This composite-modified electrode can be a candidate working electrode used in a microfluidic analytical device for determination of heavy metals in the future. Moreover, all proposed assays were applied to detect heavy metals in real samples (e.g. food, environmental and clinical samples), which the results were in good agreement with obtained from those of the standard methods.
DOI: 10.14457/cu.the.2015.490
title: MONSOON AND TROPICAL CYCLONE CHANGES DURING 1981-2013 IN COASTAL AREA THE UPPER GULF OF THAILAND

description: This study of meteorological variables those affected coastal area changes on the upper Gulf of Thailand, revealed that factors affected coastal erosion by north-eastern and south-western monsoons, which had important role for weather condition control in monsoon system blowing areas, related to changes of winds, rain and ocean currents in the Gulf of Thailand. The objectives of this research were studies of changing trend of monsoons and tropical cyclones during 1981-2013 in coastal area on the upper Gulf of Thailand and effects of relationship between changes of monsoons and tropical cyclones, which affected to coastal erosion on the upper part of the Gulf of Thailand. By using historical data from six stations of meteorology department around studied area and statistical processing with graphical plotting of changes compared to aerial satellite images of coastal erosion. The study of changing trend of monsoons from the six stations showed obvious change at Phetchaburi station. Wind blowing directions during having north eastern monsoon changed from south eastern direction and south-western monsoon changed to south-eastern and southern directions and during having north eastern monsoon conformed to increasing coastal erosion areas. Aerial satellite images showed increasing coastal change when compared to coastal erosion data from department of Marine and Coastal Resources 49.75 kilometers in Phetchaburi province, 34.69 kilometers in Samut Prakan province and 33.45 kilometers in Samut Songkhram province. For changing trend of tropical cyclones, there was no change in moving direction of the cyclones. There were low opportunities for typhoons to pass through studied area so that there was low coastal erosion from typhoons. During study tropical storm, named “Yvette 2004” affected coastal erosion in the studied area, which could be obviously seen on aerial satellite images
DOI: 10.14457/cu.the.2015.491
title: SEPARATION OF ALKYLPHENOLS BY GAS CHROMATOGRAPHY USING DERIVATIZED CYCLODEXTRINS AS STATIONARY PHASES

description: The single-run separation of twenty-two underivatized C0-C3 alkylphenols was studied by capillary gas chromatography using three derivatized cyclodextrins mixed in polysiloxane as stationary phases. These derivatized cyclodextrins were hexakis(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-alpha-cyclodextrin (ASiAc), heptakis(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-beta-cyclodextrin (BSiAc) and octakis(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-gamma-cyclodextrin (GSiAc). The results showed that three cyclodextrin-based stationary phases could be used to separate the 22 alkylphenol mixture. However, it could be suggested that the size of cyclodextrin ring affect the separation. Among three stationary phases, the medium-size BSiAc gave the best single-run separation with only two co-eluted compounds. The mixture could be separated in 8.8 minutes using a 15 m long, 0.25 mm i.d. column and a temperature program from 70-128 °C at 10 °C/min, held for 2 min; then to 220 °C at 30 °C/min. Moreover, direct analyses of the mixture without prior derivatization gave acceptable peak shapes on these three columns.
DOI: 10.14457/cu.the.2015.492
title: Development of spectroelectrochemical flow-cell for determination of caffeine content in beverages

description: A cost-effective spectroelectrochemical flow-cell was developed for determination of caffeine content in beverages. The developed flow-cell which coupled with sequential injection analysis (SIA) system can be applied for spectrophotometric and electrochemical studied in the same device. The flow-cell body was made of acrylic plastic material that is lightweight, hard and chemically inert. Its dimension was 34mm × 50mm × 15mm. The flow-cell consisted of 2 optical fiber probes with a path length of 10 mm for spectrophotometric investigated at the wavelength of 273 nm. Glassy carbon was chosen as a working electrode for caffeine oxidation studied by square wave voltammetry technique at the potential of 1.4 V vs Ag/AgCl in phosphate buffer solution. The linear ranges of caffeine determination were 0.01-0.12 mM and 0.05-0.5 mM with detection limit of 1.00 µM and 3.10 µM for spectrophotometric and electrochemical method, respectively. The reproducibility was studied and the relative standard deviation percentages (%RSD) were 3.21% and 4.98% for spectrophotometric and electrochemical measurements, respectively. This developed flow-cell is small, cost-effective and easy to connect with sequential injection analysis system. Moreover, the developed spectroelectrochemical flow-cell was successfully applied for the determination of caffeine in beverages samples and the results were insignificantly different when compare with a standard method.
DOI: 10.14457/cu.the.2015.493
title: MOLECULARLY IMPRINTED POLY(3,4-ETHYLENEDIOXYTHIOPHENE) DERIVATIVES FROM SOLID STATE POLYMERIZATION

description: Molecularly imprinted polymers (MIPs) based on poly(3,4-ethylenedioxythiophene) (PEDOT) were prepared via solid state polymerization (SSP) of 2,5-dibromo-3,4-ethylenedioxythiophene in the presence of template molecules such as p-nitrophenol (PNP), 2,4-dinitrophenol (DNP), 2,4,6-trinitrophenol (TNP) or pyrene. The conjugated MIPs exhibited the distinctive recognition of all of the externally added nitrophenol templates compared to the non-imprinted polymers (NIPs) prepared in parallel in the rebinding experiments, monitored by UV-Vis absorption. In contrast, the similarly prepared MIP using pyrene as the template showed only small difference in rebinding the nonpolar pyrene in comparison to its NIP. Similarly, molecularly imprinted copolymers (coMIPs) based on PEDOT and poly(3,4-ethylenedioxythiophene methanol) (PEDTM) were prepared using PNP as the template molecule. The resulted conjugated coMIPs with higher percentage of PEDTM showed much better recognition of externally added PNP molecules compared to the non-imprinted copolymers (coNIPs) and MIP without PEDTM. The same trend was also observed with the calculated rebinding capacities of the co-MIPs. These results indicated that the presences of polar functional groups on both template molecule and monomeric units of the MIP contribute significantly on the induced imprinting property. These accomplishments suggest a strong possibility to develop the family of PEDOT derivatives as highly specific MIP-based sensors.
DOI: 10.14457/cu.the.2015.494
title: SYNTHESIS AND POLYMERIZATION OF 3,4-DIAMINOTHIOPHENE DERIVATIVES

description: Repeated nucleophilic aromatic substitutions (SNAr) were used to synthesize new conjugated polymers. Polymer 3 was obtained from SNAr reactions of 2,5-dichloro-3,4-dinitrothiophene (DCDNT) and p-phenylenediamine as insoluble red-brown solid in 61.2% yield. Polymer 4 was also obtained from SNAr reactions of DCDNT and N,N’-di-sec-butyl-p-phenylenediamine as partially soluble black solid in 59.3% yield. The polymer 3 was then reduced by SnCl2 to obtain polymer 5 containing 3,4-diaminothiophene units in 65.4% yield. The polymer 4 was also reduced to obtain polymer 6 in 34.8% yield. To study the functionalization of the polymers through the diamino groups, the model reaction of the reduced 2,5-dibromo-3,4-dinitrothiophene (DBDNT) and benzaldehyde produced the imidazole derivative 1 in 34% yield. Similarly, reaction of the reduced DCDNT and salicylaldehyde gave the product 2 in 17% yield. With these successes on monomeric units, the polymer 7 was synthesized in 40.2% yield by consecutive reduction and condensation with benzaldehyde on polymer 4 precursor. These newly synthesized conjugated polymers exhibited the characteristic UV-Vis maximum wavelength absorptions (λmax) in the bathochromic range of 550-670 nm.
DOI: 10.14457/cu.the.2015.495
title: BUSINESS PROCESS IMPROVEMENT USING ADJUSTABLE PARAMETERS ON SIMULATION: A CASE STUDY IN RESTAURANT BUSINESS

description: The business process plays an important role in every organization. It can make a profit or loss depending on effective management procedures. Thus, in response to change inside and outside the organization, they need to improve and optimize their processes for survival and competitive advantage in the market. Business Process Improvement (BPI) is a way of improving the existing process by using process redesign heuristics. In this work, BPI methodology is adapted. An integrated simulation is performed to measure the influencing factors to the BPI. An effective number of parameters for improving the process performance characteristics are proposed by considering those which affect the slow service response time and impact on queue time, cycle time, number of task completed, and cost of operating the process. Simulation results are shown and compared with the observation data to validate, if those characteristics are valid in real situation.