cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ebppc-c7r77
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
  lga2010-01-04-3_slicer3775.jpg: Archaellum in STIV infected cell
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-3/rawdata/</p>71.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-3/rawdata/71.mrc"
        > <i class="download icon"></i> Download </a></p>

        71_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-3/3dimage_24340/71_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nejh3-62t67
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-38/rawdata/</p>39.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-38/rawdata/39.mrc"
        > <i class="download icon"></i> Download </a></p>

        39_part122_8.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-38/3dimage_24414/39_part122_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mbppe-wd294
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-18/rawdata/</p>20.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-18/rawdata/20.mrc"
        > <i class="download icon"></i> Download </a></p>

        20_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-18/3dimage_24398/20_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gwd2b-7g767
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-59/rawdata/</p>59.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-59/rawdata/59.mrc"
        > <i class="download icon"></i> Download </a></p>

        59_part122_2.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-59/3dimage_24438/59_part122_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.urhm4a
title: Occurrence Download

description: A dataset containing 18 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Calceolaria mollissima"
 ]
}

 The dataset includes 18 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006577-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bg03c-fvk18
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
  lga2010-01-04-22_slicer8544.jpg: Sulfolobus solfataricus infected with STIV. .  Budding STIV
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-22/rawdata/</p>24.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-22/rawdata/24.mrc"
        > <i class="download icon"></i> Download </a></p>

        24_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-22/3dimage_24400/24_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nd21q-efg88
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-63/rawdata/</p>62.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-63/rawdata/62.mrc"
        > <i class="download icon"></i> Download </a></p>

        62_part122_3.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-63/3dimage_24440/62_part122_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q1vgs-h9m50
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-34/rawdata/</p>35.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-34/rawdata/35.mrc"
        > <i class="download icon"></i> Download </a></p>

        35_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-34/3dimage_24411/35_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.y76ra4
title: Occurrence Download

description: A dataset containing 9757 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Senecio angulatus"
 ]
}

 The dataset includes 9757 records from 145 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006576-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.16992304
title: Pure_Li_atom_probe_dataset

description: Atom probe dataset of pure Li, made through cryo focused ion beam preperation. Datasets were collected in a Local Electrode Atom Probe (LEAP-5000 XS) a straight flight path machine.
DOI: 10.5281/zenodo.16992305
title: Pure_Li_atom_probe_dataset

description: Atom probe dataset of pure Li, made through cryo focused ion beam preperation. Datasets were collected in a Local Electrode Atom Probe (LEAP-5000 XS) a straight flight path machine.
DOI: 10.22002/dh22p-6c794
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-21/rawdata/</p>23.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-21/rawdata/23.mrc"
        > <i class="download icon"></i> Download </a></p>

        23_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-21/3dimage_24395/23_full.rec"
        > <i class="download icon"></i> Download </a></p>