DOI: 10.5061/dryad.mk6m608
title: Data from: Multi-behavioral endpoint testing of an 87-chemical compound library in freshwater planarians

description: There is an increased recognition in the field of toxicology of the value
 of medium-to-high-throughput screening methods using in vitro and
 alternative animal models. We have previously introduced the asexual
 freshwater planarian Dugesia japonica as a new alternative animal model
 and proposed that it is particularly well-suited for the study of
 developmental neurotoxicology. In this paper, we discuss how we have
 expanded and automated our screening methodology to allow for fast
 screening of multiple behavioral endpoints, developmental toxicity, and
 mortality. Using an 87-compound library provided by the National
 Toxicology Program (NTP), consisting of known and suspected
 neurotoxicants, including drugs, flame retardants, industrial chemicals,
 polycyclic aromatic hydrocarbons (PAHs), pesticides and presumptive
 negative controls, we further evaluate the benefits and limitations of the
 system for medium-throughput screening, focusing on the technical aspects
 of the system. We show that, in the context of this library, planarians
 are the most sensitive to pesticides with 16/16 compounds causing toxicity
 and the least sensitive to PAHs, with only 5/17 causing toxicity.
 Furthermore, while none of the presumptive negative controls were
 bioactive in adult planarians, 2/5, acetaminophen and acetylsalicylic
 acid, were bioactive in regenerating worms. Notably, these compounds were
 previously reported as developmentally toxic in mammalian studies. Through
 parallel screening of adults and developing animals, planarians are thus a
 useful model to detect such developmental-specific effects, which was
 observed for 13 chemicals in this library. We use the data and experience
 gained from this screen to propose guidelines for best practices when
 using planarians for toxicology screens.
description: Lethality - day 7 - 87
 compoundsThe data is each
 planarian's status at day 7. Alive planarian was marked as
 "0". Dead planarian was marked as "1". Suicide
 planarian was marked as "10".Lethality - day 12 - 87
 compoundsThe data is each
 planarian's status at day 12. Alive planarian was marked as
 "0". Dead planarian was marked as "1". Suicide
 planarian was marked as "10".Eye regeneration - day 7
 - 87 compoundsThe data is regenerated
 eyes of regenerating tails at day 7. Normal eye regeneration with 2 eyes
 regenerated was marked as "1". Abnormal eye regeneration with
 0/1/&gt;2 eyes regenerated was marked as "0". Indeterminate
 planarian (i.e. when the worm was flipped over) was marked as
 "-1". Dead/suicide planarian was marked as "NaN". Both
 Indeterminate and dead/suicide planarians were considered as
 non-analyzable planarians.Unstimulated behavior -
 day 7 - 87 compoundsThe data is
 quantification of planarian's behavior in unstimulated assay at day
 7. The speed data is the mean speed of each planarian. The resting data is
 the fraction of time planarian spent resting in the unstimulated assay.
 Non-analyzable planarians, including dead/suicide planarians, were marked
 as "NaN".Unstimulated behavior -
 day 12 - 87 compoundsThe data is
 quantification of planarian's behavior in unstimulated assay at day
 7. The speed data is the mean speed of each planarian. The resting data is
 the fraction of time planarian spent resting in the unstimulated assay.
 Non-analyzable planarians, including dead/suicide planarians, were marked
 as "NaN".Phototaxis - day 7 - 87
 compoundsThe data is the binary
 presence/absence of a response to light in phototaxis assay at day 7.
 Planarian having reacted to the light stimulus was marked as
 "1". Planarian with no reaction to light was marked as
 "0". Non-analyzable planarian was marked as
 "NaN".Phototaxis - day 12 - 87
 compoundsThe data is the binary
 presence/absence of a response to light in phototaxis assay at day 12.
 Planarian having reacted to the light stimulus was marked as
 "1". Planarian with no reaction to light was marked as
 "0". Non-analyzable planarian was marked as
 "NaN".Thermotaxis - day 12 -
 87 compoundsThe data is
 quantification of planarian's response to temperature gradient in
 thermotaxis assay at day 12, which is the fraction of time the planarian
 spent in the cold area during the last minutes of the assay. Non
 analyzable planarian was marked as "NaN".Scrunching - day 12 - 87
 compoundsThe data is the binary
 presence/absence of scrunching behavior during the scrunching assay. The
 scrunching planarian was marked as "1". The non-scrunching
 planarian was marked as "0". The non-analyzable planarian was
 marked as "NaN".Description for raw data
 filesA summary description
 for the 9 excel files of raw data.