DOI: 10.5281/zenodo.3268780
title: Systematic assessment of burst impurity in confocal-based single-molecule fluorescence detection using Brownian motion simulations - photon timetag simulation files

description: Attached are the photon timestamp and channels simulated for different 3D diffusing molecules simulations at different conditions (simulation was performed by PyBroMo).

Each of the files has, in its name, a code. The meaning of the codes are as following:

<pre>f32445 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model </pre>

<pre>a01f8f - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model</pre>

<pre>9ff667 - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model</pre>

<pre>71154a - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s - 60 second simulation using a numerical PSF model</pre>

<pre>ad926d - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 60 second simulation using a numerical PSF model</pre>

<pre>1ab235 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 180 second simulation using a numerical PSF model</pre>

<pre>d00978 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 180 second simulation using a numerical PSF model</pre>

 

<pre>2469bb - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model </pre>

<pre>4be121 - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model</pre>

<pre>a7088f - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model</pre>

<pre>023983 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s - 60 second simulation using a Gaussian PSF model</pre>

<pre>653f61 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 60 second simulation using a Gaussian PSF model</pre>

<pre>4f06ee - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 180 second simulation using a Gaussian PSF model</pre>

<pre>dec32c - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 180 second simulation using a Gaussian PSF model</pre>

 

<pre>85b0a1 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 60 second simulation using a Numerical PSF model</pre>

<pre>964ef3 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 180 second simulation using a Numerical PSF model</pre>

 

<pre>f28f6e - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 60 second simulation using a Gaussian PSF model</pre>

<pre>c311dd - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 180 second simulation using a Gaussian PSF model</pre>