cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.xwc5kx
title: Occurrence Download

description: A dataset containing 4294829 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Achillea millefolium L., Achlys triphylla (Sm.) DC., Acmispon denticulatus (Drew) D.D.Sokoloff, Actaea rubra (Aiton) Willd., Adenocaulon bicolor Hook., Adiantum aleuticum (Rupr.) C.A.Paris, Adiantum pedatum L., Agoseris aurantiaca (Hook.) Greene, Agrostis variabilis Rydb., Allotropa virgata Torr. & A.Gray, Amelanchier Medik., Amelanchier alnifolia (Nutt.) Nutt., Anaphalis margaritacea (L.) Benth., Anemonastrum deltoideum (Douglas) Mosyakin, Anemone drummondii S.Watson, Anemone L., Angelica arguta Nutt., Antennaria alpina (L.) Gaertn., Antennaria lanata (Hook.) Greene, Antennaria microphylla Rydb., Anthoxanthum nitens (Weber) Y.Schouten & Veldkamp, Anticlea occidentalis (A.Gray) Zomlefer & Judd, Apocynum androsaemifolium L., Aquilegia formosa var. formosa, Arctostaphylos nevadensis A.Gray, Arctostaphylos uva-ursi (L.) Spreng., Arnica cordifolia Hook., Arnica latifolia Bong., Arnica mollis Hook., Arnica ovata Greene, Arnica parryi A.Gray, Artemisia furcata M.Bieb., Aruncus dioicus var. acuminatus (Douglas ex Hook.) Rydb. ex H.Hara, Asarum caudatum Lindl., Asplenium trichomanes L., Aster alpinus L., Astragalus agrestis Douglas ex G.Don, Athyrium filix-femina (L.) Roth, Balsamorhiza sagittata (Pursh) Nutt., Berberis aquifolium Pursh, Berberis nervosa Pursh, Bistorta bistortoides (Pursh) Small, Struthiopteris Scop., Boechera drummondii (A.Gray) A.Löve & D.Löve, Boechera lyallii (S.Watson) Dorn, Brewerimitella breweri (A.Gray) R.A.Folk & Y.Okuyama, Bromus carinatus Hook. & Arn., Bromus sitchensis Trin., Bromus vulgaris (Hook.) Shear, Calamagrostis canadensis (Michx.) P.Beauv.)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4294829 records from 5407 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006614-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/f2fnn-pwn67
title: Rhodobacter sphaeroides JPA 1558

description: cryosections of R sphaeroides strain JPA1558 (TlpT-YFP)
description: <strong>Tilt Series Date:</strong> 2013-06-03</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grow  (1 day) in SUX medium + Naldixic acid (6.25 µl of 20mg/ml stock) + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*Spin down 5ml per strain, resuspend pellets (barely visible) in SUX medium + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*grow overnight.</p>
<strong>Sample Preparation:</strong> cryosectioning by MArk LAdinsky</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-25/rawdata/</p>atlas_grid-2_7x7cryosections0008.mrc,  Tilt Series (Pixel Size 0.38 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-25/rawdata/atlas_grid-2_7x7cryosections0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_grid-2_7x7cryosections0008_part67_7.rec,  Reconstruction (Pixel Size 0.76 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-25/3dimage_26936/atlas_grid-2_7x7cryosections0008_part67_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tr1hn-2nj27
title: Myxococcus xanthus delta-tsaP

description: grid box YWC065
description: <strong>Tilt Series Date:</strong> 2013-12-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-73/rawdata/</p>20131227_YWC065_10076.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-73/rawdata/20131227_YWC065_10076.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131227_YWC065_10076.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-73/3dimage_36836/20131227_YWC065_10076.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1dzhc-mcd82
title: Myxococcus xanthus PilO-sfGFP

description: Grid box YWC110Strain SA4088
description: <strong>Tilt Series Date:</strong> 2014-07-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-57.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor/BatchRunTomo</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 31 hr in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.6</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-48/rawdata/</p>20140716_YWC110_50010.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-48/rawdata/20140716_YWC110_50010.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140716_YWC110_50010_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-48/3dimage_44925/20140716_YWC110_50010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0ckj7-7nv18
title: Myxococcus xanthus JK328

description: Bactofilin BacMNOP knockout strain.Imaged by Janelia Titan.Forgot to put in GIF slit during data collection...
description: <strong>Tilt Series Date:</strong> 2013-07-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-07-22-15_slicer6088.jpg: unknown filament ycw2013-07-22-15_slicer6092.jpg: external vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-15/rawdata/</p>07222013_Janelia_Myxo_JK328_0015.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-15/rawdata/07222013_Janelia_Myxo_JK328_0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        07222013_Janelia_Myxo_JK328_0015_part131_6.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-15/3dimage_28457/07222013_Janelia_Myxo_JK328_0015_part131_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1t51k-9gj09
title: Myxococcus xanthus delta-pilA

description: grid box YWC059
description: <strong>Tilt Series Date:</strong> 2013-12-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-40/rawdata/</p>20131228_Janelia_YWC059_10053.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-40/rawdata/20131228_Janelia_YWC059_10053.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131228_Janelia_YWC059_10053_part131_15.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-40/3dimage_37225/20131228_Janelia_YWC059_10053_part131_15.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gq0h4-xch21
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-03-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-49/rawdata/</p>20140310_YWC092_20013.mrc,  Tilt Series (Pixel Size 3.9 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-49/rawdata/20140310_YWC092_20013.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140310_YWC092_20013_part131_10.rec,  Reconstruction (Pixel Size 15.6 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-49/3dimage_40090/20140310_YWC092_20013_part131_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9nxw1-bgx67
title: Sulfolobus cells synchronized fo

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2014-02-04</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's samples gridbox 8.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 5 sec, sponge for humidity</p>
  rra2014-02-04-16_slicer8765.jpg: pili .  Sulfolobus acidocaldarius rra2014-02-04-16_slicer8766.jpg: filament .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-16/rawdata/</p>atlas30010.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-16/rawdata/atlas30010.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas30010_part111_58.rec,  Reconstruction (Pixel Size 1.56 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-16/3dimage_38367/atlas30010_part111_58.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pxz7c-fd221
title: HeLa cells

description: Raw data files of HeLa cells
description: <strong>Tilt Series Date:</strong> 2014-11-13</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> cells and siRNA obtained from Doug Mackay/ Cathrine Ullman</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA NUP153+ Thymidine 2mM, 14 h post thymidine release</p>
  rra2014-11-13-55_slicer10583.jpg: actin bundle rra2014-11-13-55_slicer10585.jpg: tube inside a tube rra2014-11-13-55_slicer10586.jpg: tube filled with actin?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-13-55/rawdata/</p>midbody70008.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-13-55/rawdata/midbody70008.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody70008_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-13-55/3dimage_48105/midbody70008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9hfmn-gqf66
title: E.coli   "strain 4" I241E

description: E.coli chemotaxis mutant  "strain 4" I241E.Mutant locked in a CheA activating state (CW).
description: <strong>Tilt Series Date:</strong> 2012-02-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in TB/Gentamycin medium at 30 degrees, diluted in fresh media and grown to log phase. 2ml concentrated to 100µl, Penicillin added and incubated for 1 hour. Then strain was put on ice until freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot settings:100% humidity, 3µl sample, 10 nM BSA treated gold. -4 offset, 3.5s blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-29-5/rawdata/</p>strain40008.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-29-5/rawdata/strain40008.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain40008_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-29-5/3dimage_17246/strain40008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j2wc1-whb22
title: Myxococcus xanthus pilM-YFP

description: strain SA4088grid box YWC101
description: <strong>Tilt Series Date:</strong> 2014-05-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-54/3dimage_42819/</p>20140516_Janelia_YWC096_10048_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-54/3dimage_42819/20140516_Janelia_YWC096_10048_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20140516_Janelia_YWC096_10048.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-54/rawdata/20140516_Janelia_YWC096_10048.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8vyrm-28b11
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection24 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-01-07</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.4. At 24 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-07-25/rawdata/</p>stiv24h0014.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
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        stiv24h0014_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
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