cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5734779
title: Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line

description: Cancer stem cells resemble normal tissue-specific stem cells in many aspects, such as self-renewal and plasticity. Like their non-malignant counterparts, cancer stem cells are suggested to exhibit a relative quiescence. The established cancer cell lines reportedly harbor slow-proliferating cells that are positive for some cancer stem cells markers. However, the fate of these cells and their progeny remains unknown. We used time-lapse microscopy and the contrast-based segmentation algorithm to identify and monitor actively dividing and non-dividing cells in human osteosarcoma MG-63 cell line. Within the monitored field of view the non-dividing cells were represented by three cells that never divided, and one cell that attempted to divide, but failed cytokinesis, and later, after significantly prolonged division, produced the progeny with enlarged segmented nuclei, thus pointing to a possible mitotic catastrophe. Together, these cells initially constituted about 6.2% of the total number of seeded cells, yet only 0.02% of all cells at the end of the observation period when cells became confluent. Non-dividing cells were characterized by rounded shape, dark nuclei, random cytoplasmic streaming and subtle oscillatory movement, however, they did not migrate and rarely formed cell-cell contacts as compared to actively dividing cells. Our data indicate that the observed non-dividing MG-63 cells do not have a growth advantage over other cells and, therefore, they do not contribute to the cancer stem cells pool.
DOI: 10.6084/m9.figshare.5734779.v1
title: Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line

description: Cancer stem cells resemble normal tissue-specific stem cells in many aspects, such as self-renewal and plasticity. Like their non-malignant counterparts, cancer stem cells are suggested to exhibit a relative quiescence. The established cancer cell lines reportedly harbor slow-proliferating cells that are positive for some cancer stem cells markers. However, the fate of these cells and their progeny remains unknown. We used time-lapse microscopy and the contrast-based segmentation algorithm to identify and monitor actively dividing and non-dividing cells in human osteosarcoma MG-63 cell line. Within the monitored field of view the non-dividing cells were represented by three cells that never divided, and one cell that attempted to divide, but failed cytokinesis, and later, after significantly prolonged division, produced the progeny with enlarged segmented nuclei, thus pointing to a possible mitotic catastrophe. Together, these cells initially constituted about 6.2% of the total number of seeded cells, yet only 0.02% of all cells at the end of the observation period when cells became confluent. Non-dividing cells were characterized by rounded shape, dark nuclei, random cytoplasmic streaming and subtle oscillatory movement, however, they did not migrate and rarely formed cell-cell contacts as compared to actively dividing cells. Our data indicate that the observed non-dividing MG-63 cells do not have a growth advantage over other cells and, therefore, they do not contribute to the cancer stem cells pool.
DOI: 10.6084/m9.figshare.5734740
title: Spreadsheet simulation of one-dimensional unsteady-state nonisothermal moisture diffusion in wood in the transverse direction

description: A spreadsheet for the numerical simulation of one-dimensional unsteady-state nonisothermal moisture diffusion in solid wood in the transverse direction below the fiber saturation point was developed in this work. Calculations for solving a pair of partial differential equations describing the moisture diffusion based on a finite-difference method were performed. Experimental validations revealed that the spreadsheet provides predictions of moisture diffusion in wood as accurately as a commercial simulation software package does. This spreadsheet simulation can help people involved in manufacturing, utilizing, or researching wood products to implement a simple and convenient numerical simulation of moisture diffusion in wood without any special skill and any specific software.
DOI: 10.6084/m9.figshare.5734740.v1
title: Spreadsheet simulation of one-dimensional unsteady-state nonisothermal moisture diffusion in wood in the transverse direction

description: A spreadsheet for the numerical simulation of one-dimensional unsteady-state nonisothermal moisture diffusion in solid wood in the transverse direction below the fiber saturation point was developed in this work. Calculations for solving a pair of partial differential equations describing the moisture diffusion based on a finite-difference method were performed. Experimental validations revealed that the spreadsheet provides predictions of moisture diffusion in wood as accurately as a commercial simulation software package does. This spreadsheet simulation can help people involved in manufacturing, utilizing, or researching wood products to implement a simple and convenient numerical simulation of moisture diffusion in wood without any special skill and any specific software.
DOI: 10.6084/m9.figshare.5734728
title: Characterization of pulmonary responses in mice to asbestos/asbestiform fibers using gene expression profiles

description: Humans exposed to asbestos and/or asbestiform fibers are at high risk of developing many lung diseases including asbestosis, lung cancer, and malignant mesothelioma. However, the disease-causing potential and specific metabolic mechanisms and pathways associated with various asbestos/asbestiform fiber exposures triggering different carcinogenic and non-carcinogenic outcomes are still largely unknown. The aim of this this study was to investigate gene expression profiles and inflammatory responses to different asbestos/asbestiform fibers at the acute/sub-acute phase that may be related to delayed pathological outcomes observed at later time points. Mice were exposed to asbestos (crocidolite, tremolite asbestos), asbestiform fibers (erionite), and a low pathogenicity mineral fiber (wollastonite) using oropharyngeal aspiration. Similarities in inflammatory and tissue damage responses, albeit with quantitative differences, were observed at day 1 and 7 post treatment. Exposure to different fibers induced significant changes in regulation and release of a number of inflammatory cytokines/chemokines. Comparative analysis of changes in gene regulation in the lung on day 7 post exposure were interpretable in the context of differential biological responses that were consistent with histopathological findings at days 7 and 56 post treatment. Our results noted differences in the magnitudes of pulmonary responses and gene regulation consistent with pathological alterations induced by exposures to four asbestos/asbestiform fibers examined. Further comparative mechanistic studies linking early responses with the long-term endpoints may be instrumental to understanding triggering mechanisms underlying pulmonary carcinogenesis, that is lung cancer versus mesothelioma.
DOI: 10.6084/m9.figshare.5734728.v1
title: Characterization of pulmonary responses in mice to asbestos/asbestiform fibers using gene expression profiles

description: Humans exposed to asbestos and/or asbestiform fibers are at high risk of developing many lung diseases including asbestosis, lung cancer, and malignant mesothelioma. However, the disease-causing potential and specific metabolic mechanisms and pathways associated with various asbestos/asbestiform fiber exposures triggering different carcinogenic and non-carcinogenic outcomes are still largely unknown. The aim of this this study was to investigate gene expression profiles and inflammatory responses to different asbestos/asbestiform fibers at the acute/sub-acute phase that may be related to delayed pathological outcomes observed at later time points. Mice were exposed to asbestos (crocidolite, tremolite asbestos), asbestiform fibers (erionite), and a low pathogenicity mineral fiber (wollastonite) using oropharyngeal aspiration. Similarities in inflammatory and tissue damage responses, albeit with quantitative differences, were observed at day 1 and 7 post treatment. Exposure to different fibers induced significant changes in regulation and release of a number of inflammatory cytokines/chemokines. Comparative analysis of changes in gene regulation in the lung on day 7 post exposure were interpretable in the context of differential biological responses that were consistent with histopathological findings at days 7 and 56 post treatment. Our results noted differences in the magnitudes of pulmonary responses and gene regulation consistent with pathological alterations induced by exposures to four asbestos/asbestiform fibers examined. Further comparative mechanistic studies linking early responses with the long-term endpoints may be instrumental to understanding triggering mechanisms underlying pulmonary carcinogenesis, that is lung cancer versus mesothelioma.
DOI: 10.6084/m9.figshare.5734662
title: HPLC-DAD DETERMINATION OF BERBERINE, CHELERYTHRINE AND SANGUINARINE IN THE MEXICAN PRICKLY POPPY (Argemone mexicana L. PAPAVERACEAE), A MEDICINAL PLANT

description: A sensitive, simple, rapid and reliable HPLC-DAD method for the analysis of the benzylisoquinoline alkaloids (BIA's) content in Argemone mexicana (Papaveraceae) is presented. This method allows the simultaneous separation and quantitation of berberine (Bn), chelerythrine (C) and sanguinarine (S) in extracts from A. mexicana tissues, reducing time of analysis in comparison to previous reports. Alkaloids were separated on a C18 Hypersil Gold column using an acetonitrile gradient (20 to 70%) in 1% acetic acid in water. Alkaloids were identified based on retention times and UV spectra and quantified at 254 nm. Linearity between 0.5-20 µg mL-1 was observed for Bn, C and S, with limits of detection (LOD) and quantitation (LOQ) of 0.11 and 0.33 for Bn, 0.10 and 0.30 for C and 0.05 and 0.15 for S, respectively. Maximal intra- and inter-day variation values were &lt; 0.49% in all cases, with alkaloids' recoveries higher than 95%. System suitability tests (SST), including resolution (Rs), retention factor (K'), selectivity (α), tailing factor and number of theoretical plates were performed according to the United States Pharmacopeia (USP), fulfilling recommended values. The method proved to be efficient and reproducible when analyzing different tissues of field-collected A. mexicana plants.
DOI: 10.6084/m9.figshare.5734662.v1
title: HPLC-DAD DETERMINATION OF BERBERINE, CHELERYTHRINE AND SANGUINARINE IN THE MEXICAN PRICKLY POPPY (Argemone mexicana L. PAPAVERACEAE), A MEDICINAL PLANT

description: A sensitive, simple, rapid and reliable HPLC-DAD method for the analysis of the benzylisoquinoline alkaloids (BIA's) content in Argemone mexicana (Papaveraceae) is presented. This method allows the simultaneous separation and quantitation of berberine (Bn), chelerythrine (C) and sanguinarine (S) in extracts from A. mexicana tissues, reducing time of analysis in comparison to previous reports. Alkaloids were separated on a C18 Hypersil Gold column using an acetonitrile gradient (20 to 70%) in 1% acetic acid in water. Alkaloids were identified based on retention times and UV spectra and quantified at 254 nm. Linearity between 0.5-20 µg mL-1 was observed for Bn, C and S, with limits of detection (LOD) and quantitation (LOQ) of 0.11 and 0.33 for Bn, 0.10 and 0.30 for C and 0.05 and 0.15 for S, respectively. Maximal intra- and inter-day variation values were &lt; 0.49% in all cases, with alkaloids' recoveries higher than 95%. System suitability tests (SST), including resolution (Rs), retention factor (K'), selectivity (α), tailing factor and number of theoretical plates were performed according to the United States Pharmacopeia (USP), fulfilling recommended values. The method proved to be efficient and reproducible when analyzing different tissues of field-collected A. mexicana plants.
DOI: 10.6084/m9.figshare.5734656
title: DEVELOPMENT OF SENSITIVE PASSIVE SAMPLER FOR NITROGEN DIOXIDE AIR POLLUTION MONITORING

description: A passive sampler constructed with a new type of material and used for measurement of ambient nitrogen dioxide is presented. The sampler was constructed with parts of two 50 mL conical centrifuge tubes. The procedure is based on NO2 uptaken on cellulose filter impregnated with triethanolamine. After sampling, the analyte is solubilized and placed to react with Griess-Saltzman solution. The absorbance of the formed complex is determined at 540 nm. A mean relative standard deviation of 9,72% was obtained using samplers exposed in triplicate, and a determination coefficient (R2) of 0,979 was achieved in parallel measurements using the samplers and a commercial NOx monitor. Advantages of the technique include ease of use, the ready availability of components, and excellent sensitivity.
DOI: 10.6084/m9.figshare.5734656.v1
title: DEVELOPMENT OF SENSITIVE PASSIVE SAMPLER FOR NITROGEN DIOXIDE AIR POLLUTION MONITORING

description: A passive sampler constructed with a new type of material and used for measurement of ambient nitrogen dioxide is presented. The sampler was constructed with parts of two 50 mL conical centrifuge tubes. The procedure is based on NO2 uptaken on cellulose filter impregnated with triethanolamine. After sampling, the analyte is solubilized and placed to react with Griess-Saltzman solution. The absorbance of the formed complex is determined at 540 nm. A mean relative standard deviation of 9,72% was obtained using samplers exposed in triplicate, and a determination coefficient (R2) of 0,979 was achieved in parallel measurements using the samplers and a commercial NOx monitor. Advantages of the technique include ease of use, the ready availability of components, and excellent sensitivity.
DOI: 10.6084/m9.figshare.5734653
title: Adaptive Fuzzy Sliding-Mode Control of Wheel Slide Protection Device for ER24PC Locomotive

description: Abstract Wheel Slide Protection Devices (WSPD) are employed in railway vehicles to maximize the average of the possible frictional braking force, which is a nonlinear function of the slip ratio of the wheel sets. In this paper, to control the WSPD, a low-order model is presented and un-modeled dynamics are considered as uncertainties. Due to the nonlinear dynamics of the system and presence of uncertainties, Adaptive Fuzzy Sliding-Mode Control (AFSMC) is employed to regulate the slip ratio towards the desired value. The proposed controller employs a Pulse Width Modulation (PWM) technique to generate the braking torque. The second Lyapunov theorem is used to prove the closed-loop asymptotic stability. In the simulations, the switching dynamics of WSPD is considered and the multi-body dynamics method is used for modeling the longitudinal dynamics of ER24PC locomotive. The obtained results reveal that by using the AFSMC method, the slip ratios of wheel sets converge to the reference values. Unlike the conventional method, in which the fluctuations of slip ratio diverge near the stopping time, simulation studies reveal that with the AFSMC method, the stopping time of the locomotive and the fluctuation amplitude of the slip ratios are reduced.
DOI: 10.6084/m9.figshare.5734653.v1
title: Adaptive Fuzzy Sliding-Mode Control of Wheel Slide Protection Device for ER24PC Locomotive

description: Abstract Wheel Slide Protection Devices (WSPD) are employed in railway vehicles to maximize the average of the possible frictional braking force, which is a nonlinear function of the slip ratio of the wheel sets. In this paper, to control the WSPD, a low-order model is presented and un-modeled dynamics are considered as uncertainties. Due to the nonlinear dynamics of the system and presence of uncertainties, Adaptive Fuzzy Sliding-Mode Control (AFSMC) is employed to regulate the slip ratio towards the desired value. The proposed controller employs a Pulse Width Modulation (PWM) technique to generate the braking torque. The second Lyapunov theorem is used to prove the closed-loop asymptotic stability. In the simulations, the switching dynamics of WSPD is considered and the multi-body dynamics method is used for modeling the longitudinal dynamics of ER24PC locomotive. The obtained results reveal that by using the AFSMC method, the slip ratios of wheel sets converge to the reference values. Unlike the conventional method, in which the fluctuations of slip ratio diverge near the stopping time, simulation studies reveal that with the AFSMC method, the stopping time of the locomotive and the fluctuation amplitude of the slip ratios are reduced.
DOI: 10.6084/m9.figshare.5734647
title: Experimental Investigation of Steel-Concrete Bond for Thin Reinforcing Bars

description: Abstract The steel-concrete bond is a fundamental property in reinforced concrete structures. Although there are several studies on the steel-concrete bond, few of them have evaluated the performance of reinforcing bars with diameters less than 10.0 mm, which includes 5.0, 6.3, and 8.0 mm diameters, which are normally used in reinforced-concrete elements. This study experimentally evaluates the bond between thin steel bars and concrete of 25MPa compression strength. Three types of methods of testing the bond-strength were performed: confined bar test, pull-out test and beam test. It was compared the adequacy of the tests to calculate the conformation coefficient of the bars. The results of the confined bars tests show that this test may be inadequate to determine the surface conformation coefficient of reinforcing bars thinner than 10 mm, especially for notched (CA-60) steel bars. The pull-out test resulted in better results in terms of evaluating the bond behavior. Regarding the specimens for the pull-out tests, a modified model with an anchorage length equal to 10 times the bar diameter is suggested. Therefore, the main contribution of this study, based on the results obtained and the methodology used, is to present a proposal for the evaluation of steel-concrete bond for thin rebars.
DOI: 10.6084/m9.figshare.5734647.v1
title: Experimental Investigation of Steel-Concrete Bond for Thin Reinforcing Bars

description: Abstract The steel-concrete bond is a fundamental property in reinforced concrete structures. Although there are several studies on the steel-concrete bond, few of them have evaluated the performance of reinforcing bars with diameters less than 10.0 mm, which includes 5.0, 6.3, and 8.0 mm diameters, which are normally used in reinforced-concrete elements. This study experimentally evaluates the bond between thin steel bars and concrete of 25MPa compression strength. Three types of methods of testing the bond-strength were performed: confined bar test, pull-out test and beam test. It was compared the adequacy of the tests to calculate the conformation coefficient of the bars. The results of the confined bars tests show that this test may be inadequate to determine the surface conformation coefficient of reinforcing bars thinner than 10 mm, especially for notched (CA-60) steel bars. The pull-out test resulted in better results in terms of evaluating the bond behavior. Regarding the specimens for the pull-out tests, a modified model with an anchorage length equal to 10 times the bar diameter is suggested. Therefore, the main contribution of this study, based on the results obtained and the methodology used, is to present a proposal for the evaluation of steel-concrete bond for thin rebars.
DOI: 10.6084/m9.figshare.5734644
title: The use of subtitled films in teaching and learning foreign languages: vocabulary acquisition in English

description: Abstract This paper addresses vocabulary acquisition in English through the implementation of a pedagogic routine, which we call here didactic sequence (DS), that is oriented to be used in foreign language (FL) classes. This DS involves the work with subtitled films and written translations in a functional perspective, focusing on vocabulary acquisition in FL. In order to investigate the effectiveness of the DS, a research was conducted with a high school class from a public school in Fortaleza, Ceará. The research measured the participant’s vocabulary acquisition in English, which reached the average of 56,48% when taken from the lexical shortage (32,75%) identified in the pre-test.
DOI: 10.6084/m9.figshare.5734644.v1
title: The use of subtitled films in teaching and learning foreign languages: vocabulary acquisition in English

description: Abstract This paper addresses vocabulary acquisition in English through the implementation of a pedagogic routine, which we call here didactic sequence (DS), that is oriented to be used in foreign language (FL) classes. This DS involves the work with subtitled films and written translations in a functional perspective, focusing on vocabulary acquisition in FL. In order to investigate the effectiveness of the DS, a research was conducted with a high school class from a public school in Fortaleza, Ceará. The research measured the participant’s vocabulary acquisition in English, which reached the average of 56,48% when taken from the lexical shortage (32,75%) identified in the pre-test.
DOI: 10.6084/m9.figshare.5734641
title: Rollover Index for the Diagnosis of Tripped and Untripped Rollovers

description: Abstract It is necessary to detect danger as soon as possible to avoid rollover of a vehicle in sudden events. Using rollover index in real time can be used for this purpose. The traditional rollover indices currently applying in the vehicles can only detect the untripped rollover due to severe lateral acceleration in vehicles. These indices cannot detect the tripped rollover resulted from vertical external forces in a long direction. There are recently many quantitative studies about the tripped rollover and an index was also introduced to this kind of rollover. In this research, we examined the dynamics of a SUV to improve this index and also presented a new index to detect the both types of rollovers. The precision and accuracy of the new index was evaluated by simulation in industrial software of Carsim. The numerical results of the new developed model were compared with the test results of an automobile at one-eighth scale in equal conditions and inputs. The results are indicative of the better performance of the new model presented in this research.
DOI: 10.6084/m9.figshare.5734641.v1
title: Rollover Index for the Diagnosis of Tripped and Untripped Rollovers

description: Abstract It is necessary to detect danger as soon as possible to avoid rollover of a vehicle in sudden events. Using rollover index in real time can be used for this purpose. The traditional rollover indices currently applying in the vehicles can only detect the untripped rollover due to severe lateral acceleration in vehicles. These indices cannot detect the tripped rollover resulted from vertical external forces in a long direction. There are recently many quantitative studies about the tripped rollover and an index was also introduced to this kind of rollover. In this research, we examined the dynamics of a SUV to improve this index and also presented a new index to detect the both types of rollovers. The precision and accuracy of the new index was evaluated by simulation in industrial software of Carsim. The numerical results of the new developed model were compared with the test results of an automobile at one-eighth scale in equal conditions and inputs. The results are indicative of the better performance of the new model presented in this research.