DOI: 10.5061/dryad.kd51c5bd1
title: Multigenetic phylogeny of the subfamily Larentiinae (Lepidoptera: Geometridae)

description: This dataset is part of a paper, which demonstrates how micro-CT can be
 applied to provide unambiguous illustrations of diagnostic morphological
 characters for new taxa description and to understand how micro-CT imaging
 may complement other imaging techniques. Following micro-CT scanning, a
 semi-automatic segmentation and volume rendering protocol was used to
 portray the wing venation and diagnostic structures and ornamentation of
 male genitalia from multiple angles. Using micro-CT images, we describe a
 conspicuous geometrid moth from southern Africa (Lepidoptera:
 Geometridae), which has been present in collections since 1894 but left
 without an available name. Using a multigenetic dataset comprising 273
 terminal taxa from the superfamily Geometroidea, we constructed a
 molecular phylogeny to place our study species to an isolated lineage in
 Geometridae: Larentiinae, tribe Xanthorhoini sensu lato. We describe it as
 Chloecolora vergetaria new genus, new species Englund & Staude,
 and provide diverse ecological information on its distribution, habitat,
 host plant, adult and immature stages, and parasites.
description: One entire mid-leg or femur, when no complete leg was available,
 was detached from the <em>Chloecolora vergetaria</em> sp. n.
 specimens for DNA extraction. Genomic DNA was extracted using the Qiagen
 DNeasy Blood and Tissue Kit following the manufacturer’s protocol at the
 Finnish Museum of Natural History (FMNH) molecular laboratory. DNA
 amplification and sequencing were carried out following protocols proposed
 by Wahlberg and Wheat (2008): one mitochondrial gene (mtCOI) and six
 protein-coding nuclear gene regions, Arginine Kinase (ArgK),
 sarco/endoplasmic reticulum calcium ATPase (Ca-ATPase), Elongation Factor
 1 alpha (EF-1alpha), sorting Nexin-9-like (Nex9), Ribosomal Protein
 (RpS5), and wingless (wgl).  The molecular data
 extracted from three specimens were included in the dataset of a total of
 273 terminal taxa. The remaining 272 terminal taxa, with up to 11 genes
 per species, were obtained from published data by Murillo-Ramos
 (Murillo-Ramos et al., 2019). All subfamilies of the family Geometridae
 were represented in the dataset, and two species from the family Uraniidae
 were set as an outgroup. To construct the phylogenetic
 trees, we applied the maximum likelihood approach as implemented in the
 IQ-TREE web server (Trifinopoulos et al., 2016). Best-fitting substitution
 models were selected by ModelFinder (Kalyaanamoorthy et al., 2017) with
 the “-m MFP+MERGE” option. The phylogenetic analyses were carried out with
 the “-spp” option (edge proportional), which allowed each partition to
 have its own evolutionary rate. We evaluated the node supports with
 ultrafast bootstrap approximations (UFBoot2) and SH-like approximate
 likelihood ratio test (Guindon et al., 2010; Hoang et al., 2018) using the
 “-B 1000 -alrt 1000” option. To minimize the risk of overestimating branch
 supports in ultrafast bootstrap approximation analysis, we used the
 “-bnni” option, which optimized each bootstrap tree using a hill-climbing
 nearest-neighbour-interchange (NNI) search.
description: # Larentiinae\_Full\_Tree\_FigS4; Multigenetic phylogeny of the subfamily
 Larentiinae (Lepidoptera: Geometridae)
 [https://doi.org/10.5061/dryad.kd51c5bd1](https://doi.org/10.5061/dryad.kd51c5bd1) The data file contains fasta and tree files used to construct a molecular phylogeny of the subfamily Larentiinae (Lepidoptera: Geometridae). It is part of the manuscript "130 years from discovery to description: micro-CT scanning applied to construct the integrative taxonomy of a forgotten moth from Southern Africa (Lepidoptera: Geometridae)" that was submitted for referral in a scientific journal as of 2.11.2023. ## Description of the data and file structure The tree consists of 275 protein-coding nuclear and mitochondrial sequences from 273 terminal taxa from the superfamily Geometroidae (Lepidoptera); 2 of them from Uranidae forming an outgroup and the rest from Geometridae, 195 from the subfamily Larentiinae. ## Sharing/Access information The tree contains novel sequences from three specimens of a new Larentine moth species, *Chloecolora vergetaria* sp.n., the rest originating from previously published datasets. More specific data-sharing info will become available upon publication of the original article. ## Code/Software References for the used software can be found in the forthcoming original article.