cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/te1wy-fez26
title: MT310_xyl(+)_Cd50uM(+)

description: Raw data files of MT310_xyl(+)_Cd50uM(+)
description: <strong>Tilt Series Date:</strong> 2009-06-09</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> The MT310 strain was constructed by Martin Thanbichler; cells were cultured and treated by Zhuo Li; data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were induced by xylose and treated with 50 uM CdCl2 for 20 hours.</p>
<strong>Sample Preparation:</strong> 10 nm gold. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-38/rawdata/</p>09jun09a_Cd50uM_057.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-38/rawdata/09jun09a_Cd50uM_057.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun09a_Cd50uM_057_full.rec,  Reconstruction (Pixel Size 1.9 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-38/3dimage_5179/09jun09a_Cd50uM_057_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.vt6epa
title: Occurrence Download

description: A dataset containing 216 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Colombia",
 "GadmGid is COL.15_2",
 "OccurrenceStatus is Present",
 "TaxonKey is Quercus humboldtii"
 ]
}

 The dataset includes 216 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048396-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/pr2r2-2d250
title: Mycobacter smegmatis pneumonia

description: KP3 WT (S) M. smegmatis mc2_155
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> mc2_155</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF Tomo  gatan K2  Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul / Eric Brown Genentech, Inc. 1 DNA Way South San Francisco, CA 94080 (650) 225-6248 Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Saunton Media (S)</p>
<strong>Sample Preparation:</strong> 3ul + 10nm Au R2/2 quantifoils 4.5 offset 1 blot 2s 1s drainsingle tilt. constant angular increment, step: 1. tilt range: (-60, 60).        dosage: 180/A2. defocus: -8um. magnification: 26000</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-31/rawdata/</p>atlas10031.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-31/rawdata/atlas10031.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10031_part121_5.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-31/3dimage_30625/atlas10031_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tamb4-qn182
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC117
description: <strong>Tilt Series Date:</strong> 2014-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 180 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-28-29/rawdata/</p>20140928_YWC117_20013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-28-29/rawdata/20140928_YWC117_20013.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140928_YWC117_20013_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-28-29/3dimage_46813/20140928_YWC117_20013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.u5cyvf
title: Occurrence Download

description: A dataset containing 27 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Symphyotrichum novae-angliae",
 "Country is Slovakia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 27 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048399-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/knecg-q8w38
title: Mycobacter smegmatis pneumonia

description: KP3 WT (S) M. smegmatis mc2_155
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> mc2_155</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF Tomo  gatan K2  Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul / Eric Brown Genentech, Inc. 1 DNA Way South San Francisco, CA 94080 (650) 225-6248 Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Saunton Media (S)</p>
<strong>Sample Preparation:</strong> 3ul + 10nm Au R2/2 quantifoils 4.5 offset 1 blot 2s 1s drainsingle tilt. constant angular increment, step: 1. tilt range: (-60, 60).        dosage: 180/A2. defocus: -8um. magnification: 26000</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-27/rawdata/</p>atlas10027.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-27/rawdata/atlas10027.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10027_part121_3.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-27/3dimage_30612/atlas10027_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y5cq4-j1e32
title: CenK Sacculus

description: C. crescentus CenK sacculus .
description: <strong>Tilt Series Date:</strong> 2007-05-05</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 2/2, BSA treated 10nm gold, 100% humidity, -4mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-2/rawdata/</p>CenK_03.mrc,  Tilt Series (Pixel Size 0.96 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-2/rawdata/CenK_03.mrc"
        > <i class="download icon"></i> Download </a></p>

        CenK_03.rec,  Reconstruction (Pixel Size 0.96 nm), 720.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-2/3dimage_3006/CenK_03.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.29964332
title: Supporting Information for “No transcontinental paleo-Red River flowing to the South China Sea: A mass balance perspective”

description: Supporting Information for "<b>No transcontinental paleo-Red River flowing to the South China Sea: A mass balance perspective"</b>
DOI: 10.6084/m9.figshare.29964332.v2
title: Supporting Information for “No transcontinental paleo-Red River flowing to the South China Sea: A mass balance perspective”

description: Supporting Information for "<b>No transcontinental paleo-Red River flowing to the South China Sea: A mass balance perspective"</b>
DOI: 10.6084/m9.figshare.29964332.v1
title: Supporting Information for “<b>No transcontinental paleo-Red River flowing to the South China Sea: A mass balance perspective”</b>

description: Supporting Information for "<b>No transcontinental paleo-Red River flowing to the South China Sea: A mass balance perspective"</b>
DOI: 10.22002/4a87k-chm44
title: Helicobacter

description: T4SS search
description: <strong>Tilt Series Date:</strong> 2007-11-06</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Helicobacter hepaticus</p>
<strong>Strain:</strong> ATCC 51449</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1 degree increment, cos°, constant angular increment, dosage: 2278.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Janet Chow, Mazmanian Lab; prepared Helicobacter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> WT grown in culture and centrifuged to pellet</p>
<strong>Sample Preparation:</strong> R 2/1 quantifoil grids, 10 nm gold, 100% humidity, box 463</p>
  mdj2007-11-06-3_slicer4478.jpg: Flagellar motor mdj2007-11-06-3_slicer4479.jpg: Extracellular vesicle mdj2007-11-06-3_slicer4555.jpg: Chemoreceptors mdj2007-11-06-3_slicer4557.jpg: Flagellar motors mdj2007-11-06-3_slicer4559.jpg: Extracellular decorated vesicle
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2007-11-06-3/3dimage_2940/</p>hh07nov06t03_full.rec,  Reconstruction (Pixel Size 15.64 nm), 179.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2007-11-06-3/3dimage_2940/hh07nov06t03_full.rec"
        > <i class="download icon"></i> Download </a></p>

        mdj2007-11-06-3_slicer16439.png,  REZ .  -andrew, 267.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/mdj2007-11-06-3_slicer16439.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x7dtt-jtz42
title: CCcenK_glu

description: C. crescentus CenK, glucose depletion .
description: <strong>Tilt Series Date:</strong> 2006-07-06</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-2/rawdata/</p>CCcenK_glu02.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-2/rawdata/CCcenK_glu02.mrc"
        > <i class="download icon"></i> Download </a></p>

        CCcenK_glu02.rec,  Reconstruction (Pixel Size 2.4 nm), 308.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-2/3dimage_2946/CCcenK_glu02.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2gzmaf
title: Occurrence Download

description: A dataset containing 37587 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acer pictum"
 ]
}

 The dataset includes 37587 records from 297 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048395-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/z5fe3-wv676
title: Hyphomonas "AR43"

description: ParA-like protein, HNE0708, venus
description: <strong>Tilt Series Date:</strong> 2012-03-04</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in Marine Broth (MB) medium at 30 degrees. Expression of Venus labeled HNK0708 was induced by adding final 300 uM CuCl2 and incubated at 30 degrees for 24 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2012-03-04-5_slicer8704.jpg: REZ .  Ribosome exclusion zone .  Hyphomonas neptunium
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-03-04-5/rawdata/</p>2012Mar04_AR43_24hr_10003.mrc,  Tilt Series (Pixel Size 0.644 nm), 842.5 MB  
        
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        > <i class="download icon"></i> Download </a></p>

        2012Mar04_AR43_24hr_10003_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bjqbg-4py28
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC114
description: <strong>Tilt Series Date:</strong> 2014-08-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-30/rawdata/</p>20140811_YWC114_50003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-30/rawdata/20140811_YWC114_50003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140811_YWC114_50003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-30/3dimage_45504/20140811_YWC114_50003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6zvy1-tmg35
title: Hyphomonas "AR43"

description: ParA-like protein, HNE0708, venus
description: <strong>Tilt Series Date:</strong> 2012-03-04</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in Marine Broth (MB) medium at 30 degrees. Expression of Venus labeled HNK0708 was induced by adding final 300 uM CuCl2 and incubated at 30 degrees for 24 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2012-03-04-26_slicer8734.jpg: Hyphomonas neptunium .  ribosome exclusion zone
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-03-04-26/rawdata/</p>2012Mar04_AR43_24hr_30020.mrc,  Tilt Series (Pixel Size 0.644 nm), 842.5 MB  
        
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        > <i class="download icon"></i> Download </a></p>

        2012Mar04_AR43_24hr_30020_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ggm23-5k243
title: Myxococcus xanthus DK1622 wt

description: For T4P averaging
description: <strong>Tilt Series Date:</strong> 2013-08-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-92/rawdata/</p>20130826_DK1622_WT_30034.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
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        20130826_DK1622_WT_30034_part131_5.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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