cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7542737
title: Screening of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing in twin pregnancies

description: <b>Objectives</b>: This study was aimed to report the clinical characteristics of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing (NIPT) in twin pregnancies and analyze the results in terms of chorionicity, conception, and fetal fraction. <b>Methods</b>: A total of 1160 women with twin pregnancies were recruited from 1 October 2015, to 1 August 2017. Next-generation sequencing technology was used to detect fetal aneuploidies, such as trisomy 21, trisomy 18, trisomy 13 and trisomy X. <b>Results</b>: Aneuploidy was detected using NIPT in 26 fetuses, among which 18 fetal aneuploidies occurred in only one fetus of the twins. The rate of aneuploidy was 1.3% for dichorionic diamniotic twins and 0.5% for monochorionic diamniotic twins, respectively. The rate of aneuploidy was 1.2% for spontaneous pregnancy group and 1.1% for assisted reproductive technologies group. <b>Conclusion</b>: In this study, detection of trisomy 21, trisomy 18, trisomy 13, and X abnormality in twin pregnancies was confirmed to be accurate. The aneuploidies mostly occurred in only one fetus of the twins, and trisomy 21 was the most common type. The prenatal diagnostic standard for NIPT in singleton pregnancies could perform well in twin pregnancies, which means NIPT can be popularized as routine prenatal screening in twin pregnancies.
DOI: 10.6084/m9.figshare.7542737.v1
title: Screening of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing in twin pregnancies

description: <b>Objectives</b>: This study was aimed to report the clinical characteristics of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing (NIPT) in twin pregnancies and analyze the results in terms of chorionicity, conception, and fetal fraction. <b>Methods</b>: A total of 1160 women with twin pregnancies were recruited from 1 October 2015, to 1 August 2017. Next-generation sequencing technology was used to detect fetal aneuploidies, such as trisomy 21, trisomy 18, trisomy 13 and trisomy X. <b>Results</b>: Aneuploidy was detected using NIPT in 26 fetuses, among which 18 fetal aneuploidies occurred in only one fetus of the twins. The rate of aneuploidy was 1.3% for dichorionic diamniotic twins and 0.5% for monochorionic diamniotic twins, respectively. The rate of aneuploidy was 1.2% for spontaneous pregnancy group and 1.1% for assisted reproductive technologies group. <b>Conclusion</b>: In this study, detection of trisomy 21, trisomy 18, trisomy 13, and X abnormality in twin pregnancies was confirmed to be accurate. The aneuploidies mostly occurred in only one fetus of the twins, and trisomy 21 was the most common type. The prenatal diagnostic standard for NIPT in singleton pregnancies could perform well in twin pregnancies, which means NIPT can be popularized as routine prenatal screening in twin pregnancies.
DOI: 10.6084/m9.figshare.12261815.v1
title: Organic farming promotes selective uptake of glycine over nitrate uptake by pakchoi

description: Understanding how plants use of various nitrogen (N) sources is important for improving plant N use efficiency in organic farming systems. This study investigated the effects of farming management practices (organic and conventional) on pakchoi short-term uptake of glycine (Gly), nitrate (NO<sub>3</sub><sup>−</sup>) and ammonium (NH<sub>4</sub><sup>+</sup>) under two N level conditions. Results showed that plant N uptake rates and N contributions from the three N forms in the low N (0.15 μg N g<sup>−1</sup> dry soil) treatment did not significantly differ between the organic and conventional soils, except the significantly greater Gly contribution in organic soil at 24 h after tracer addition. Under high N (15 μg N g<sup>−1</sup> dry soil) conditions, the N uptake rates, uptake efficiencies, and N contributions of Gly and NH<sub>4</sub><sup>+</sup>-N were significantly greater in pakchoi cultivated in the organic soil compared to conventional soil, whereas the N uptake rates and N contributions from NO<sub>3</sub><sup>–</sup>-N decreased in pakchoi cultivated in the organic soil. The greater Gly-N uptake in plants grown in high-N treated organic soil may be related to the greater gross N transformation, Gly turnover rate and the increased expression of an amino acid transporter gene <i>BcLHT1</i>. Intact Gly contributed at most 6% to Gly-derived N at 24 h after tracer additions, which accounting for about 1.24% of the total N uptake in organic soil. Our study suggested that Gly-N and other organic source N might serve as a more important compensatory N source for plants in organic farming.
DOI: 10.6084/m9.figshare.19601574
title: <i>In-situ</i> observation of deformation twin associated sub-grain boundary formation in copper single crystal under bending

description: In this work, we use <i>in-situ</i> TEM in combination with finite elemental analysis to reveal the sub-grain boundary formation during bending of Cu single crystal. The deformation process is featured by dislocations propagation, formation of stacking faults and deformation twins, and subsequent formation of a sub-grain boundary. Finite element analysis of stress distribution indicates the stacking faults and deformation twins are all initiated from the region that corresponds to the maximum shear stress. The formation of the sub-grain boundary is through the intersection between primary and secondary stacking faults/deformation twins. Real-time observation of the plastic deformation process during the bending of Cu single crystal, reveals the sub-grain boundary formation through the intersection between primary and secondary stacking faults/deformation twins.
DOI: 10.25345/c5mg7g640
title: MassIVE MSV000094068 - Characterization of Complex Proteoform Mixtures by Online Nanoflow Ion-Exchange Chromatography - Native Mass Spectrometry
title: This dataset was used to support the project "Characterization of Complex Proteoform Mixtures by Online Nanoflow Ion-Exchange Chromatography - Native Mass Spectrometry".
DOI: 10.6084/m9.figshare.19588827
title: Additional file 1 of Multi-trait genomic prediction using in-season physiological parameters increases prediction accuracy of complex traits in US wheat

description: Additional file 1.
DOI: 10.6084/m9.figshare.14870249.v1
title: Additional file 1 of DNA methylation-based sex classifier to predict sex and identify sex chromosome aneuploidy

description: Additional file 1 Sex related differentially methylated CpGs. A list of the identified 4331 sex related differentially methylated CpGs on sex chromoseomes which are also used to construct the classifier.
DOI: 10.6084/m9.figshare.14870251.v1
title: Additional file 10 of Genome-wide identification and expression analysis of LBD transcription factor genes in Moso bamboo (Phyllostachys edulis)

description: Additional file 10: Supplemental Data 2. The specific primers of PeLBD genes for qRT-PCR.
DOI: 10.6084/m9.figshare.14870254
title: Additional file 11 of Genome-wide identification and expression analysis of LBD transcription factor genes in Moso bamboo (Phyllostachys edulis)

description: Additional file 11: Supplemental Data 3. Detailed information on gene ontology enrichment analysis in PeLBD family.
DOI: 10.6084/m9.figshare.19588827.v1
title: Additional file 1 of Multi-trait genomic prediction using in-season physiological parameters increases prediction accuracy of complex traits in US wheat

description: Additional file 1.
DOI: 10.6084/m9.figshare.7542947
title: Balancing yield, purity and practicality: a modified differential ultracentrifugation protocol for efficient isolation of small extracellular vesicles from human serum

description: Ultracentrifugation remains the gold standard for isolation of small extracellular vesicles (sEV), particularly for cancer applications. The objective of this study was to determine if a widely used ultracentrifugation protocol for isolation of serum sEV could be modified to reduce the number of ultracentrifugation cycles and increase efficiency, while maintaining equal or better sample purity and yield. Serum was obtained from two healthy subjects. sEVs were isolated from 1 mL aliquots using three different ultracentrifugation protocols. Co-isolation of RNA carrier protein was assessed by performing Western blots for ApoA-I, ApoB, and Ago2. Small RNA-sequencing was performed on the sEV isolates, and differential detection of small ncRNA was compared across isolation protocols. Reduction from three- to two-ultracentrifuge cycles with no sucrose cushion resulted in a much higher sEV yield but also had the highest levels of lipoprotein and Ago2 contamination. However, the two-ultracentrifugation cycle protocol that incorporated a 30% sucrose cushion into the first cycle resulted in slightly higher sEV yields with lower levels of protein contamination compared to the lengthier three-ultracentrifugation cycle approach, therefore presenting a more efficient alternative approach for isolation of serum sEVs. It was also notable that there were some differences in sEV ncRNA cargo according to protocol, although it was less than expected given the differences in co-isolated RNA carrier proteins. Our results suggest that use of the modified serum sEV isolation protocol with two ultracentrifugation cycles and incorporating a 30% sucrose cushion offers a more efficient approach in terms of efficiency and purity.
DOI: 10.6084/m9.figshare.19601574.v1
title: <i>In-situ</i> observation of deformation twin associated sub-grain boundary formation in copper single crystal under bending

description: In this work, we use <i>in-situ</i> TEM in combination with finite elemental analysis to reveal the sub-grain boundary formation during bending of Cu single crystal. The deformation process is featured by dislocations propagation, formation of stacking faults and deformation twins, and subsequent formation of a sub-grain boundary. Finite element analysis of stress distribution indicates the stacking faults and deformation twins are all initiated from the region that corresponds to the maximum shear stress. The formation of the sub-grain boundary is through the intersection between primary and secondary stacking faults/deformation twins. Real-time observation of the plastic deformation process during the bending of Cu single crystal, reveals the sub-grain boundary formation through the intersection between primary and secondary stacking faults/deformation twins.
DOI: 10.5281/zenodo.10653815
title: plasmid and its gene catalog in the early life human gut study

description: the plasmid and its gene catalog were generated from 9520 fecal samples worldwide, mainly from infants, using an in-house ensemble workflow (https://github.com/Wanli-HE/Plaspline) .
DOI: 10.5281/zenodo.10653816
title: plasmid and its gene catalog in the early life human gut study

description: the plasmid and its gene catalog were generated from 9520 fecal samples worldwide, mainly from infants, using an in-house ensemble workflow (https://github.com/Wanli-HE/Plaspline) .
DOI: 10.6084/m9.figshare.13310143
title: Additional file 12 of Effect of diet and intestinal AhR expression on fecal microbiome and metabolomic profiles

description: Additional file 12: Table S8. Differentially abundant metabolites between AhRKO and WT mice fed LFD at week 26.
DOI: 10.15468/dl.dnvpp7
title: Occurrence Download

description: A dataset containing 276070 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Eryngium campestre"
 ]
}

 The dataset includes 276070 records from 1762 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010555-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15156/bio/sh1199572.09fu
title: SH1199572.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199573.09fu
title: SH1199573.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199574.09fu
title: SH1199574.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199575.09fu
title: SH1199575.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199576.09fu
title: SH1199576.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199577.09fu
title: SH1199577.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199578.09fu
title: SH1199578.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199579.09fu
title: SH1199579.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199580.09fu
title: SH1199580.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh1199581.09fu
title: SH1199581.09FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.