cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25381/cput.22494517.v1
title: The influence of change in organisational structure on employee performance at a private sector organisation in cape town

description: Ethical Clearance reference: 2017FBREC459 <strong>1.</strong> <strong>Interview questions and responses</strong> <strong>2.</strong> <strong>Survey questions and results</strong>
DOI: 10.6084/m9.figshare.23256323
title: Search data by country

description: Search data by country
DOI: 10.6084/m9.figshare.23256323.v1
title: Search data by country

description: Search data by country
DOI: 10.25381/cput.22560991
title: The role of leader behaviour on project team performance in the execution of construction projects

description: <strong>2021_FBMSREC 097 </strong>The dataset is split into three sections: Section A – Biography, Section B – Likert scale, and Section C–open-ended questions. Each section had several questions for different information purposes. Section A’s main focus was on biography to assess the study participants’ levels, importance, and understanding. Section B was a Likert scale used to evaluate variables such as perceptions, expectations, experiences and gave respondents a chance to express their views on how their leaders conduct themselves and what they would love to see from their project leaders. While the last section, Section C, was made of closed and open-ended questions designed to give respondents space to respond and express themselves thoroughly without being restricted to a set of questions.
DOI: 10.25381/cput.22560991.v1
title: The role of leader behaviour on project team performance in the execution of construction projects

description: <strong>2021_FBMSREC 097 </strong>The dataset is split into three sections: Section A – Biography, Section B – Likert scale, and Section C–open-ended questions. Each section had several questions for different information purposes. Section A’s main focus was on biography to assess the study participants’ levels, importance, and understanding. Section B was a Likert scale used to evaluate variables such as perceptions, expectations, experiences and gave respondents a chance to express their views on how their leaders conduct themselves and what they would love to see from their project leaders. While the last section, Section C, was made of closed and open-ended questions designed to give respondents space to respond and express themselves thoroughly without being restricted to a set of questions.
DOI: 10.25381/cput.22284520
title: An adaptive design approach to studio spaces in higher education within the Interior Design and Architectural disciplines

description: Ethical Reference no: 210032464/2022/9 This research is focused on dedicated learning spaces within higher education institutions and, more specifically, studio spaces within the disciplines of Interior Design and Architectural education. Conventional design studios in higher education are manifesting a shift due to the steady progress of the internet and information technology. Thus, the educational function of the design studio within the traditional pedagogical structure needs to respond to current social, economic, ecological and technological changes. Consequently, the main research challenge that prompted this study was the search for, and conceptualisation of, a creative and adaptive approach to a more collaborative studio space, so that the integration of the physical studio space and virtual studio might work together to complement each other. The reinvention of the physical studio space could ultimately lead to the design principle of a more structured hot?desking studio, founded on mobile work patterns. Moreover, this research suggests the concept of a nomadic studio as a further development of hot-desking which institutions could adopt. The research follows a qualitative approach which employed Participatory Action Research. In order to analyse the user responses within these spaces, focus group discussions, interviews and participatory observation were carried out at a private higher education institution that has embraced an adaptive and technology-based studio environment. The findings revealed notable needs for adaptation regarding the architecture, interior and furniture elements within the identified spatial typologies of a studio. In particular, tangibility and tacit knowledge were also emphasised by the findings, namely that the immersive nomadic studio which offers students the opportunity to be present in the ‘real world’ while designing for the ‘real world’ makes a positive contribution towards empathic design needs.
DOI: 10.25381/cput.22284520.v1
title: An adaptive design approach to studio spaces in higher education within the Interior Design and Architectural disciplines

description: Ethical Reference no: 210032464/2022/9 This research is focused on dedicated learning spaces within higher education institutions and, more specifically, studio spaces within the disciplines of Interior Design and Architectural education. Conventional design studios in higher education are manifesting a shift due to the steady progress of the internet and information technology. Thus, the educational function of the design studio within the traditional pedagogical structure needs to respond to current social, economic, ecological and technological changes. Consequently, the main research challenge that prompted this study was the search for, and conceptualisation of, a creative and adaptive approach to a more collaborative studio space, so that the integration of the physical studio space and virtual studio might work together to complement each other. The reinvention of the physical studio space could ultimately lead to the design principle of a more structured hot?desking studio, founded on mobile work patterns. Moreover, this research suggests the concept of a nomadic studio as a further development of hot-desking which institutions could adopt. The research follows a qualitative approach which employed Participatory Action Research. In order to analyse the user responses within these spaces, focus group discussions, interviews and participatory observation were carried out at a private higher education institution that has embraced an adaptive and technology-based studio environment. The findings revealed notable needs for adaptation regarding the architecture, interior and furniture elements within the identified spatial typologies of a studio. In particular, tangibility and tacit knowledge were also emphasised by the findings, namely that the immersive nomadic studio which offers students the opportunity to be present in the ‘real world’ while designing for the ‘real world’ makes a positive contribution towards empathic design needs.
DOI: 10.17632/42mzcbtk3h.1
title: Data for: An Environmental Scan of Online Resources for Informal Family Caregivers of ICU Survivors 

description: This set contains a table of results from our environmental scan, undertaken to identify and collate a comprehensive repository of online resources on subjects considered by experts and service users as important to family caregivers of ICU survivors. We searched for online resources using social media, professional society websites, and the internet using Google as well as contacting our professional networks to identify additional resources. Information categories identified by experts and service users were developed by the project team. Our search identified 301 online resources which were grouped into informational categories according to the content of each online resource. The online resources identified are listed and described. The information category each online resource fulfils is indicated by an ‘X’.
DOI: 10.5281/zenodo.7979747
title: Data set related to the article: "SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line."

description: Abstract: SARS-CoV-2 infection causes a considerable inflammatory response coupled with impaired platelet reactivity, which can lead to platelet disorders recognized as negative prognostic factors in COVID-19 patients. The virus may cause thrombocytopenia or thrombocytosis during the different disease stages by destroying or activating platelets and influencing platelet production. While it is known that several viruses can impair megakaryopoiesis by generating an improper production and activation of platelets, the potential involvement of SARS-CoV-2 in affecting megakaryopoiesis is poorly understood. To this purpose, we explored, in vitro, the impact of SARS-CoV-2 stimulation in the MEG-01 cell line, a human megakaryoblastic leukemia cell line, considering its spontaneous capacity of releasing platelet-like particles (PLPs). We interrogated the effect of heat-inactivated SARS- CoV-2 lysate in the release of PLPs and activation from MEG-01, the signaling pathway influenced by SARS-CoV-2, and the functional effect on macrophagic skewing. The results highlight the potential influence of SARS-CoV-2 in the early stages of megakaryopoiesis by enhancing the production and activation of platelets, very likely due to the impairment of STATs signaling and AMPK activity. Overall, these findings provide new insight into the role of SARS-CoV-2 in affecting megakaryocyte– platelet compartment, possibly unlocking another avenue by which SARS-CoV-2 moves.
DOI: 10.5281/zenodo.7979748
title: Data set related to the article: "SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line."

description: Abstract: SARS-CoV-2 infection causes a considerable inflammatory response coupled with impaired platelet reactivity, which can lead to platelet disorders recognized as negative prognostic factors in COVID-19 patients. The virus may cause thrombocytopenia or thrombocytosis during the different disease stages by destroying or activating platelets and influencing platelet production. While it is known that several viruses can impair megakaryopoiesis by generating an improper production and activation of platelets, the potential involvement of SARS-CoV-2 in affecting megakaryopoiesis is poorly understood. To this purpose, we explored, in vitro, the impact of SARS-CoV-2 stimulation in the MEG-01 cell line, a human megakaryoblastic leukemia cell line, considering its spontaneous capacity of releasing platelet-like particles (PLPs). We interrogated the effect of heat-inactivated SARS- CoV-2 lysate in the release of PLPs and activation from MEG-01, the signaling pathway influenced by SARS-CoV-2, and the functional effect on macrophagic skewing. The results highlight the potential influence of SARS-CoV-2 in the early stages of megakaryopoiesis by enhancing the production and activation of platelets, very likely due to the impairment of STATs signaling and AMPK activity. Overall, these findings provide new insight into the role of SARS-CoV-2 in affecting megakaryocyte– platelet compartment, possibly unlocking another avenue by which SARS-CoV-2 moves.
DOI: 10.5281/zenodo.7979727
title: Data set related to the article: "The Role of BPIFB4 in Immune System and Cardiovascular Disease: The Lesson from Centenarians."

description: Abstract: Recent discoveries have shed light on the participation of the immune system in the physio pathology of the car- diovascular system underpinning the importance of keeping the balance of the first to preserve the latter. Aging, along with other risk factors, can challenge such balance triggering the onset of cardiovascular diseases. Among several mediators ensuring the proper cross-talk between the two systems, bactericidal/permeability- increasing fold-containing family B member 4 (BPIFB4) has been shown to have a pivotal role, also by sustaining important signals such as eNOS and PKC-alpha. In addition, the Longevity-associated variant (LAV), which is an haplotype allele in BPIFB4 characterized by 4 missense polymorphisms, enriched in homozygosity in Long Living Individuals (LLIs), has been shown to be efficient, if admin- istered systemically through gene therapy, in improving many aspects of cardiovascular diseases (CVDs). This occurs mainly through a fine immune system remodeling across: 1) a M2 macrophage polarizing effect, 2) a favorable redistri- bution of the circulating monocyte cell subsets and 3) the reduction of T-cell activation. Furthermore, LAV-BPIFB4 treat- ment induced a desirable recovery of the inflammatory balance by mitigating the pro-inflammatory factor levels and enhancing the anti-inflammatory boost through a mechanism that is partially dependent on SDF-1/CXCR4 axis. Importantly, the remarkable effects of LAV-BPIFB4 treatment, which translates in increased BPIFB4 circulating levels, mirror what occurs in long-living individuals (LLIs) in whom the high circulating levels of BPIFB4 are protective from age-related and CVDs and emphasize the reason why LLIs are considered a model of successful aging. Here, we review the mechanisms by which LAV-BPIFB4 exerts its immunomodulatory activity in improving the cardiovascular-immune system dialogue that might strengthen its role as a key mediator in CVDs.
DOI: 10.5281/zenodo.7979728
title: Data set related to the article: "The Role of BPIFB4 in Immune System and Cardiovascular Disease: The Lesson from Centenarians."

description: Abstract: Recent discoveries have shed light on the participation of the immune system in the physio pathology of the car- diovascular system underpinning the importance of keeping the balance of the first to preserve the latter. Aging, along with other risk factors, can challenge such balance triggering the onset of cardiovascular diseases. Among several mediators ensuring the proper cross-talk between the two systems, bactericidal/permeability- increasing fold-containing family B member 4 (BPIFB4) has been shown to have a pivotal role, also by sustaining important signals such as eNOS and PKC-alpha. In addition, the Longevity-associated variant (LAV), which is an haplotype allele in BPIFB4 characterized by 4 missense polymorphisms, enriched in homozygosity in Long Living Individuals (LLIs), has been shown to be efficient, if admin- istered systemically through gene therapy, in improving many aspects of cardiovascular diseases (CVDs). This occurs mainly through a fine immune system remodeling across: 1) a M2 macrophage polarizing effect, 2) a favorable redistri- bution of the circulating monocyte cell subsets and 3) the reduction of T-cell activation. Furthermore, LAV-BPIFB4 treat- ment induced a desirable recovery of the inflammatory balance by mitigating the pro-inflammatory factor levels and enhancing the anti-inflammatory boost through a mechanism that is partially dependent on SDF-1/CXCR4 axis. Importantly, the remarkable effects of LAV-BPIFB4 treatment, which translates in increased BPIFB4 circulating levels, mirror what occurs in long-living individuals (LLIs) in whom the high circulating levels of BPIFB4 are protective from age-related and CVDs and emphasize the reason why LLIs are considered a model of successful aging. Here, we review the mechanisms by which LAV-BPIFB4 exerts its immunomodulatory activity in improving the cardiovascular-immune system dialogue that might strengthen its role as a key mediator in CVDs.
DOI: 10.5281/zenodo.7979218
title: Data set related to the article: "The longevity-associated BPIFB4 gene supports cardiac function and vascularization in ageing cardiomyopathy"

description: <strong>Aims</strong> The ageing heart naturally incurs a progressive decline in function and perfusion that available treatments cannot halt. However, some exceptional individuals maintain good health until the very late stage of their life due to favourable gene–environment inter- action. We have previously shown that carriers of a longevity-associated variant (LAV) of the BPIFB4 gene enjoy prolonged health spans and lesser cardiovascular complications. Moreover, supplementation of LAV-BPIFB4 via an adeno-associated viral vector im- proves cardiovascular performance in limb ischaemia, atherosclerosis, and diabetes models. Here, we asked whether the LAV- BPIFB4 gene could address the unmet therapeutic need to delay the heart’s spontaneous ageing. <strong>Methods and results</strong> Immunohistological studies showed a remarkable reduction in vessel coverage by pericytes in failing hearts explanted from elderly patients. This defect was attenuated in patients carrying the homozygous LAV-BPIFB4 genotype. Moreover, pericytes isolated from older hearts showed low levels of BPIFB4, depressed pro-angiogenic activity, and loss of ribosome biogenesis. LAV-BPIFB4 supple- mentation restored pericyte function and pericyte-endothelial cell interactions through a mechanism involving the nucleolar protein nucleolin. Conversely, BPIFB4 silencing in normal pericytes mimed the heart failure pericytes. Finally, gene therapy with LAV-BPIFB4 prevented cardiac deterioration in middle-aged mice and rescued cardiac function and myocardial perfusion in older mice by improving microvasculature density and pericyte coverage. <strong>Conclusions</strong> We report the success of the LAV-BPIFB4 gene/protein in improving homeostatic processes in the heart’s ageing. These findings open to using LAV-BPIFB4 to reverse the decline of heart performance in older people.
DOI: 10.5281/zenodo.7979219
title: Data set related to the article: "The longevity-associated BPIFB4 gene supports cardiac function and vascularization in ageing cardiomyopathy"

description: <strong>Aims</strong> The ageing heart naturally incurs a progressive decline in function and perfusion that available treatments cannot halt. However, some exceptional individuals maintain good health until the very late stage of their life due to favourable gene–environment inter- action. We have previously shown that carriers of a longevity-associated variant (LAV) of the BPIFB4 gene enjoy prolonged health spans and lesser cardiovascular complications. Moreover, supplementation of LAV-BPIFB4 via an adeno-associated viral vector im- proves cardiovascular performance in limb ischaemia, atherosclerosis, and diabetes models. Here, we asked whether the LAV- BPIFB4 gene could address the unmet therapeutic need to delay the heart’s spontaneous ageing. <strong>Methods and results</strong> Immunohistological studies showed a remarkable reduction in vessel coverage by pericytes in failing hearts explanted from elderly patients. This defect was attenuated in patients carrying the homozygous LAV-BPIFB4 genotype. Moreover, pericytes isolated from older hearts showed low levels of BPIFB4, depressed pro-angiogenic activity, and loss of ribosome biogenesis. LAV-BPIFB4 supple- mentation restored pericyte function and pericyte-endothelial cell interactions through a mechanism involving the nucleolar protein nucleolin. Conversely, BPIFB4 silencing in normal pericytes mimed the heart failure pericytes. Finally, gene therapy with LAV-BPIFB4 prevented cardiac deterioration in middle-aged mice and rescued cardiac function and myocardial perfusion in older mice by improving microvasculature density and pericyte coverage. <strong>Conclusions</strong> We report the success of the LAV-BPIFB4 gene/protein in improving homeostatic processes in the heart’s ageing. These findings open to using LAV-BPIFB4 to reverse the decline of heart performance in older people.
DOI: 10.6084/m9.figshare.21369992
title: Impact of interleukin-32α on T helper cell-related cytokines, transcription factors, and proliferation in patients with type 2 diabetes mellitus

description: The ability of interleukin (IL)-32α to induce T helper (Th) 1, Th17, and Treg cytokines (interferon gamma [IFN-γ], IL-17, and IL-10, respectively), and transcription factors ([signal transducer and activator of transcription (<i>STAT</i>) 1 and <i>T-box</i> (<i>T-bet</i>) for Th1, <i>STAT3</i> and retinoid-related orphan receptor (<i>ROR</i>)<i>-γt</i> for Th17, and <i>STAT5</i> and forkhead box P3 (<i>Foxp3</i>) for Treg]) were investigated in type 2 diabetes mellitus (T2DM). IL-32α effects on Th cell proliferation and related factors including IL-2 and <i>NF-κB</i> were also explored. Serum levels of IL-32α in 31 patients and 31 healthy controls (HCs) were determined by ELISA assay. CD4<sup>+</sup> T cells cultured with polyclonal activators in the presence and absence of recombinant IL-32α (rIL-32α). Gene expressions in cultured Th cells were assessed with real-time PCR. Cytokines in supernatants were measured with ELISA. Proliferation experiments were assessed by flow cytometry. The patients showed significant increase in IL-32α levels compared with HCs and its levels were positively correlated with fasting plasma glucose (FPG) and hemoglobin A1c (HbA1c). rIL-32α enhanced IL-17 and IL-2 production, increased <i>ROR-γt</i> and <i>NF-κB</i> expression, and enhanced Th proliferation in both patients and HCs. In patients, IL-17, <i>ROR-γt</i>, <i>NF-κB,</i> and proliferation levels were higher than those in HCs, in cultures with and without rIL-32α (rIL-32α<sup>+</sup> and rIL-32α<sup>-</sup>). IL-2 levels in rIL-32α<sup>+</sup>cultures of patients were significantly higher than the HCs, and it was positively correlated with proliferation rate and <i>NF-κB</i> expression. Aberrant IL-32α levels are participated in T2DM pathogenesis. IL-32α potently induces Th17-related factors and amplifies the proliferative function of T cells.HighlightsEnhanced serum levels of IL-32α in T2DM patients was correlated with FPG and HbA1c.IL-32α increases <i>ROR-γt</i> expression and IL-17 production, and induces Th17 cells.IL-32α enhances <i>NF-κB</i> expression and IL-2 production, and promotes Th proliferation.IL-32α is more effective for inducing Th17 cells and proliferation in the patients.IL-32α axis could be mentioned as a future therapeutic goal for the T2DM. Enhanced serum levels of IL-32α in T2DM patients was correlated with FPG and HbA1c. IL-32α increases <i>ROR-γt</i> expression and IL-17 production, and induces Th17 cells. IL-32α enhances <i>NF-κB</i> expression and IL-2 production, and promotes Th proliferation. IL-32α is more effective for inducing Th17 cells and proliferation in the patients. IL-32α axis could be mentioned as a future therapeutic goal for the T2DM.