cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/wk4c1-jty97
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-110/rawdata/</p>RCC745_RELtovis_119.mrc,  Tilt Series (Pixel Size 1.26 nm), 545.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-110/rawdata/RCC745_RELtovis_119.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_119.rec,  Reconstruction (Pixel Size 1.26 nm), 190.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-110/3dimage_12561/RCC745_RELtovis_119.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tp4wm-7gk46
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-07-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-84/3dimage_11595/</p>RCC745_MGtovis_257.rec,  Reconstruction (Pixel Size 1.26 nm), 186.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-84/3dimage_11595/RCC745_MGtovis_257.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_257.mrc,  Tilt Series (Pixel Size 1.26 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-84/rawdata/RCC745_MGtovis_257.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/59a14-zhz61
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-04-25</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-04-25-36/3dimage_11316/</p>RCC745_MGtovis_039.rec,  Reconstruction (Pixel Size 1.26 nm), 513.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-04-25-36/3dimage_11316/RCC745_MGtovis_039.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_039.mrc,  Tilt Series (Pixel Size 1.26 nm), 688.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-04-25-36/rawdata/RCC745_MGtovis_039.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mkmq6-hjz55
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-151/rawdata/</p>RCC745_RELtovis_163.mrc,  Tilt Series (Pixel Size 1.26 nm), 562.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-151/rawdata/RCC745_RELtovis_163.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_163.rec,  Reconstruction (Pixel Size 1.26 nm), 544.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-151/3dimage_12643/RCC745_RELtovis_163.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.28342025.v2
title: <b>Comprehensive Single-Cell Transcriptomic Atlas of the Mouse Pons and Medulla</b>

description: This integrated dataset represents the large-scale transcriptomics cell atlas of pons and medulla, compiled from 8 independent single-cell/single-nucleus RNA sequencing (sc/sn-RNA seq) datasets. This integration was performed using a standardized bioinformatic workflow, clustering, and marker-based annotation. This dataset comprised 317 985 quality-passed<code></code> cells with 45 cell types.This dataset offers a valuable source for single-cell neuroscience to understand region-specific molecular insights and their cellular diversity.To aid interpretability, annotations are structured hierarchically into three levels:<b>Level 1</b>: Broad categories (e.g., Neurons, Astrocytes, Oligodendrocytes)<b>Level 2</b>: Subtypes within broad categories (e.g., Excitatory neurons, Inhibitory neurons)<b>Level 3</b>: Fine-grained subtypes (e.g., Glut1, Glut2, GABA1, GABA2).We provide the following datasets to facilitate reuse:<b>Final_Integrated_Pons_Medulla.rds</b>Contains all neuronal and non-neuronal populations.The <code>cell_type</code> column includes Level 1, Level 2, and Level 3 annotations combined.<b>Pons_Medulla_Neurons_level_3.rds</b>Subset of neuronal populations.Includes <b>Level 2 and Level 3 annotations</b> for neurons in the <code>cell_type</code> column.<b>GABA_Gluta.rds</b>Subset of <b>excitatory (glutamatergic)</b> and <b>inhibitory (GABAergic)</b> neuronal populations.Includes <b>Level 3 annotations</b> for detailed analysis of these key neurotransmitter systems.
DOI: 10.22002/f1gc3-z9z86
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-62/rawdata/</p>RCC745_MGtovis_377.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-62/rawdata/RCC745_MGtovis_377.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_377.rec,  Reconstruction (Pixel Size 1.26 nm), 171.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-62/3dimage_11735/RCC745_MGtovis_377.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.f3wqv6
title: Occurrence Download

description: A dataset containing 22251 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Callitriche hamulata"
 ]
}

 The dataset includes 22251 records from 401 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048254-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/9d7yp-ykv17
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-11-16</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-31/rawdata/</p>RCC745_MGtovis_432.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-31/rawdata/RCC745_MGtovis_432.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_432.rec,  Reconstruction (Pixel Size 1.26 nm), 367.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-31/3dimage_11827/RCC745_MGtovis_432.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16956047
title: The 347 curated chemolithoautotrophs genome list.

description: The curated list contains 359 chemolithoautotrophic genomes, of which 347 were used for benchmarking AutoFixMark against the other two tools, as described in the AutoFixMark paper.
DOI: 10.22002/npsep-zsq65
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-09-30</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-68.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-54/rawdata/</p>RCC745_G1tovis_203.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-54/rawdata/RCC745_G1tovis_203.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_203.rec,  Reconstruction (Pixel Size 1.26 nm), 269.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-54/3dimage_12195/RCC745_G1tovis_203.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m8t6w-7at33
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-09-30</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-68.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-42/rawdata/</p>RCC745_G1tovis_189.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-42/rawdata/RCC745_G1tovis_189.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_189.rec,  Reconstruction (Pixel Size 1.26 nm), 338.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-42/3dimage_12171/RCC745_G1tovis_189.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a5xec6
title: Occurrence Download

description: A dataset containing 52 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Rudbeckia hirta",
 "Country is Czechia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 52 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048259-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/eqg3s-wmj52
title: Hippocampal Neurons (cultured)

description: These tomograms were shot at -4 um defocus with total dose of 80 electrons.
description: <strong>Tilt Series Date:</strong> 2014-12-07</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-07-1/3dimage_48608/</p>120514_hipp_neuron_square80001_part121_2.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-07-1/3dimage_48608/120514_hipp_neuron_square80001_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        120514_hipp_neuron_square80001.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-07-1/rawdata/120514_hipp_neuron_square80001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nkrrx-q1w65
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-74/rawdata/</p>RCC745_MGtovis_392.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-74/rawdata/RCC745_MGtovis_392.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_392.rec,  Reconstruction (Pixel Size 1.26 nm), 226.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-74/3dimage_11759/RCC745_MGtovis_392.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/559j8-20v10
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-07-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-47/3dimage_11521/</p>RCC745_MGtovis_180.rec,  Reconstruction (Pixel Size 1.26 nm), 109.5 MB  
        
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        RCC745_MGtovis_180.mrc,  Tilt Series (Pixel Size 1.26 nm), 536.9 MB  
        
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