cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0nz86z2
title: CIL:38870, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The cytosol contains numerous vacuoles as well as symbiotic bacteria (see Heckmann, Int. Rev. Cytol. 14(suppl.):111-143, 1983 for a discussion of symbiotic bacteria in Euplotes). TEM taken on 7/24/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0qr4whj
title: CIL:38869, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: View of the packed layer of mitochondria just under the dorsal pellicle of Euplotes is prominent. A row of eight cilia can be seen along one kinety arising from round to oval depressions in the cell‚Äôs surface. A grid bar was deleted on the left. TEM taken on 8/1/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,800X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0h994jf
title: CIL:38868, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: A grazing section through the dorsal surface of Euplotes showing cross sections of cilia in four of the sparsely ciliated kineties . A layer of mitochondria underlies the pellicle. A replication band of the DNA in the macronucleus is typical of hypotrichs. The basal bodies and cilia of one cirrus are found as well as the contractile vacuole. EM taken on 8/1/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,800X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0w0958k
title: CIL:38867, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Euplotes sp. (eurystomus ?) is a hypotrich. This ciliate has a few rows of dorsal cilia as well as specialized clumps of cilia called cirri on its ventral side and a complex of membranelles involved in feeding called the adoral zone of membranelles. The cell is flattened dorso-ventrally. This electron micrograph is a longitudinal section dividing dorsal from ventral parts of the cell and exposing the oral cavity, the thread-like macronucleus, the extended contractile vacuole, contractile vacuole pore, and a large assortment of food vacuoles many of which contained material that was lost during sectioning leaving holes in the section. The cytopharynx and nascent food vacuole along with the discoidal (pharyngeal) vesicles are also seen. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,370X. Bar = 5µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0zw1jtv
title: CIL:38905, sperm. In Cell Image Library

description: Cross section through a seminiferous tubule showing the developing sperm with their tails pointing into the lumen of the tubule. The nuclei are stained blue with DAPI and mitochondria red with mitotracker. Sperm have a large number of mitochondria to power their swim towards the egg. The image has been volume rendered using Imaris.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); mitotracker
Processing history: volume rendering
description: B0006849. Seminiferous tubule . Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j0n29w8s
title: CIL:38899, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Cross sections of a pair of basal bodies showing the triplet makeup of the 9 fibers; only one microtubular fiber is complete in each triplet while the other two microtubular fibers share some subunits with their neighbors. TEM taken on 2/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 47,100X.
DOI: 10.6075/j0pz57gc
title: CIL:38896, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Septa of bordering alveoli when lying in the plane of section exhibit pores at their outer margins closest to the plasma membrane. This shows that there is physical continuity between the lumens of alveoli. Basal bodies and fibrous systems are found in the cellular cortex beneath the ridges. Cacodylate buffered fixative. TEM taken on 11/2/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Published in J. Cell Biol. 49:1-20, 1971. Adapted with permission.
DOI: 10.6075/j0qc02b2
title: CIL:38911, Urtica dioica, leaf. In Cell Image Library

description: Scanning electron micrograph of stinging hairs on the surface of a nettle leaf. The large stinging hairs (trichomes) are hollow tubes with walls of silica making them into tiny glass needles. The bulb at the base of each hair contains the stinging liquid that includes formic acid, histamine, acetylcholine and 5- hydroxytryptamine (serotonin). The tips of the glassy hairs are very easily broken when brushed, leaving a sharp point, which easily pierces the skin to deliver the sting.
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
description: B0006134 Scanning electron micrograph 1993 Collection: Wellcome Images. Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j0cz3604
title: CIL:38938, squamous carcinoma. In Cell Image Library

description: Scanning electron micrograph of the inside of a cancer cell. This cell originates from a squamous cell carcinoma, a type of skin cancer. The cell has been frozen and split open to reveal its nucleus.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
description: B0006269 Cell from a squamous cell carcinoma. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j04q7sv9
title: CIL:38908, Cavia porcellus, cochlear inner hair cell. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
description: B0003553 Scanning electron micrograph Collection: Wellcome Images Library reference no.: Contributor Reference B0000110B. Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Color-enhanced scanning electron micrograph of the inside of a guinea pig inner ear showing the hearing organ, or cochlea. Running along the spiral structure are rows of sensory cells which respond to different frequencies of sound. The whole organ is just a few millimeters long.
DOI: 10.6075/j0mg7nbv
title: CIL:38907, Rattus, astrocyte, oligodendrocyte, neuronal stem cell. In Cell Image Library

description: B0006485. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Processing history: unprocessed raw data
description: Confocal micrograph of astrocytes and oligodendrocytes from neural stem cells Astrocytes (green) and oligodendrocytes (red) derived from rat neural stem cells in culture. Both these cells are types of neuroglia and are important for correct brain function.
DOI: 10.6075/j0j38rch
title: CIL:38916, thalamus. In Cell Image Library

description: B0002689 Credit MRC Toxicology Unit. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: This confocal image of normal brain tissue from the thalamus is stained with antibodies to receptors for orexin (red). Orexins are peptide hormones that help keep us awake and alert, and may also stimulate the appetite. They are produced in the hypothalamus and act through their receptors located in the nuclei of cells in many different parts of the brain. The green stain highlights the neurofilaments and the blue stain, the nuclei. A drug called orexin-RA-1 that blocks the action of these receptors is currently under development as a sleeping pill. It may also have the effect of reducing the appetite.
description: Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Processing history: unprocessed raw data
DOI: 10.6075/j0j96570
title: CIL:38915, Homo sapiens. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: atomic force microscopy
Parameter imaged: elevation
Source of contrast: differences in height
Visualization methods: visualization of contiguous regions
Processing history: colorized
Data qualification: Processed
description: B0003664 Scanning probe image of chromosome 1. Wellcome Images Library reference no.: Contributor Reference B0001890C. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Scanning probe/atomic force microscope image of human chromosome 1. Additional scanning probe chromosome images are available as CIL 38987, 39000, and 39013.
DOI: 10.6075/j0tb15rn
title: CIL:38914, Homo sapiens, mucin cells. In Cell Image Library

description: B0003960 Wellcome Images Colonic crypts - typical hexagonal shapes. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Confocal image of colonic crypts in cross section. Mucin cells (yellow) in the crypts produce mucous which passes into the interior of the colon through the crypt lumen (the white spots in the centres of the crypts) to facilitate the movement of the faecal mass through the colon. Staining for F-actin (blue) shows the pericryptal sheaths. This tissue is from an endoscopic colonic biopsy for a child and shows the normal appearence of the colonic crypts.
description: Relation to intact cell: sectioned tissue; endoscopic biopsy
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Processing history: colorized
DOI: 10.6075/j04q7swr
title: CIL:38912, Homo sapiens, epithelial cell of cervix. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Processing history: unprocessed raw data
description: B0004985 Confocal micrograph 2003 Collection: Wellcome Images. Image available thru following license http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Human HeLa cancer cells in culture showing the nuclei in red and the tubulin component of the cytoskeleton in green. The blue staining is a mitotic checkpoint protein that remains in the cytoplasm until pro-metaphase when it is involved in regulating cell division. HeLa cells are derived from a human cervical cancer and can be propogated indefinitely in culture.
DOI: 10.6075/j0j965pt
title: CIL:37396, damselfly, flight muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.
 
 Technical details:
 Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.
 
 Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199
DOI: 10.6075/j0hm573f
title: CIL:37934, Caenorhabditis elegans, early embryonic cell. In Cell Image Library

description: C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with latrunculin A. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video2 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; time lapse microscopy
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: mCherryFP; EGFP; Hoechst
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug latrunculin A (disrupts the actin cytoskeleton) at frame 13. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.
DOI: 10.6075/j0f18z2b
title: CIL:37395, damselfly, flight muscle cell. In Cell Image Library

description: Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.
 
 Technical details:
 Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.
 
 Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199
description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0nc60jk
title: CIL:37394, damselfly, flight muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.
 
 Technical details:
 Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.
 
 Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199