DOI: 10.5281/zenodo.17608462
title: Raw data of the article "Effects of kefirs made from whole milk or whey on the pancreas-intestine axis in a mouse model of acute pancreatitis"

description:  Description of the project

 

Background: Acute pancreatitis (AP) is a severe inflammatory condition, often involving

intestinal barrier dysfunction. Fermented foods like kefir, are potential therapeutic adjuvants

due to their gut-modulatory properties. This study aimed to compare the protective effects of

kefir made from pasteurized whole milk (PMK) or whey (WK) on pancreatic injury, intestinal

barrier integrity, inflammation, and oxidative stress in a cerulein-induced mouse model of AP.

Methods: Male BALB/c mice were assigned to six groups: Control, AP, PMK, WK, and AP

combined with either PMK or WK. AP was induced by cerulein injections on days 10 and 11. Mice

received kefir or saline via oral gavage for 14 days. Pancreatic and ileal tissues were analyzed for

histopathological damage, serum amylase and lipase levels, gene expression of inflammatory

and tight junction markers (TNF-α, IL-6, IL-1β, Occludin, ZO-1), oxidative stress (MDA, carbonyls),

and intestinal function. Results: AP induction was confirmed by elevated serum amylase/lipase

and severe pancreatic damage. While both PMK and WK partially reduced pancreatic

histopathological damage, they failed to reduce serum amylase and lipase levels. Furthermore,

PMK, but not WK, partially attenuated pancreatic IL-6 and IL-1β expression, and both kefirs

reduced ileal TNF-α. However, neither kefir prevented the AP-induced downregulation of tight

junction genes, the increase in oxidative stress markers in either tissue, or the impairment of

intestinal transit and contractility. Conclusion: Kefir supplementation offers localized anti-

inflammatory benefits in a severe AP model. It is insufficient to mitigate key systemic markers

of pancreatic injury, restore gut barrier integrity, or reverse functional dysmotility. These

findings suggest that the therapeutic potential of kefir may be limited in the context of severe

acute inflammatory conditions.

 

 Description of the dataset

 

This dataset contains the experimental data associated with Figures 3 and 5-9 of the mentioned article. 

 

 

Files included

 



-Raw data Figure 3.xlsx — experimental raw data for Figure 3

 

-Raw data Figure 5.xlsx — experimental raw data for Figure 5

 

-Raw data Figure 6.xlsx — experimental raw data for Figure 6

 

-Raw Data Figure 7.xlsx — experimental raw data for Figure 7

 

-Raw Data Figure 8.xlsx — experimental raw data for Figure 8

 

-Raw Data Figure 9.xlsx — experimental raw data for Figure 9

 

 

 

-Processed data Figure 3.xlsx — experimental Processed data for Figure 3

 

-Processed data Figure 5.xlsx — experimental Processed data for Figure 5

 

-Processed data Figure 6.xlsx — experimental Processed data for Figure 6

 

-Processed Data Figure 7.xlsx — experimental Processed data for Figure 7

 

-Processed Data Figure 8.xlsx — experimental Processed data for Figure 8

 

-Processed Data Figure 9.xlsx — experimental Processed data for Figure 9

 


 

 

README.txt — this document