DOI: 10.34894/gogwgh
title: Replication Data for: Expressed protein profile of a Tectomicrobium and other microbial symbionts in the marine sponge Aplysina aerophoba as evidenced by metaproteomics

description: &lt;p&gt;Data files generated within the project described in the paper: “Expressed protein profile of a “&lt;i&gt;Candidatus&lt;/i&gt; Entotheonella”-like bacterium and other microbial symbionts in the marine sponge &lt;i&gt;Aplysina aerophoba&lt;/I&gt; as evidenced by metaproteomics”.&lt;br/&gt;
Data is organized in two zip archives &lt;b.1_Raw_data.zip&lt;/b&gt; and &lt;b&gt;2_Processed_data.zip&lt;/b&gt;.&lt;/p&gt;
&lt;p&gt;&lt;b&gt;Contents of the archives&lt;/b&gt;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;1_Raw_data.zip&lt;/b&gt;:&lt;br/&gt;The directory &lt;b&gt;peaks_exports&lt;/b&gt; contains the information related to the &lt;i&gt;de novo&lt;/i&gt; sequencing of the peptides. There is one subdirectory for each sample (10 in total). Each sample subdirectory contains 5 files:&lt;/p&gt;
&lt;ul&gt;
&lt;li&gt;&lt;b&gt;DB search psm.csv&lt;/b&gt;: Output of the database search including post-translational modifications (PTM)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;de novo only peptides.csv&lt;/b&gt;: Features of de novo peptides only&lt;/li&gt;
&lt;li&gt;&lt;b&gt;peptide.csv&lt;/b&gt;: Features of peptides identified&lt;/li&gt;
&lt;li&gt;&lt;b&gt;protein-peptides.csv&lt;/b&gt;: Features of peptides matching proteins in the database&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins.csv&lt;/b&gt;: Features of all identified proteins&lt;/li&gt;
&lt;/ul&gt;
&lt;p&gt;&lt;b&gt;2_Processed_data.zip&lt;/b&gt;:&lt;br/&gt;The archive contains two subfolders:&lt;br/&gt;
&lt;b&gt;1_Assemblies_databases&lt;/b&gt;: Metagenomic assemblies (contigs &gt; 1 Kbp) used as a database for matching sequenced peptides. There are two, one for the sponge &lt;b&gt;aply_sponge_1k_aa.faa&lt;/b&gt;, and one for the seawater &lt;b&gt;sw_1k_aa.faa&lt;/b&gt;&lt;br/&gt;
&lt;b&gt;2_Protein_fastas&lt;/b&gt;: Includes the actual input for the analyses presented, and similarly is divided into two subfolders: &lt;b&gt;Seawater_Mediterranean&lt;/b&gt;, containing four files, one for each seawater sample analyzed; and &lt;b&gt;Sponge_Aplysina_aerophoba&lt;/b&gt; which includes six files, one for each sponge sample analyzed.&lt;br/&gt;All files are named according to sample identifiers provided in the manuscript, namely:&lt;/p&gt;
&lt;ul&gt;
&lt;li&gt;&lt;b&gt;DB search psm.csv&lt;/b&gt;: Output of the database search including post-translational modifications (PTM)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_16B.fasta&lt;/b&gt; (Sponge Aplysina aerophoba)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_16C.fasta&lt;/b&gt; (Sponge Aplysina aerophoba)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_17.fasta&lt;/b&gt; (Sponge Aplysina aerophoba)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_19A.fasta&lt;/b&gt; (Sponge Aplysina aerophoba)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_19B.fasta&lt;/b&gt; (Sponge Aplysina aerophoba)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_25.fasta&lt;/b&gt; (Sponge Aplysina aerophoba)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_sw5.fasta&lt;/b&gt; (Mediterranean seawater)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_sw6.fasta&lt;/b&gt; (Mediterranean seawater)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_sw7.fasta&lt;/b&gt; (Mediterranean seawater)&lt;/li&gt;
&lt;li&gt;&lt;b&gt;proteins_sw8.fasta&lt;/b&gt; (Mediterranean seawater)&lt;/li&gt;
&lt;/ul&gt;