cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/m0e3h-0q759
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-14/rawdata/</p>tomo6.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-14/rawdata/tomo6.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo6_part59_5.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-14/3dimage_114923/tomo6_part59_5.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo6_part59_4.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-14/3dimage_114970/tomo6_part59_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/894jp-x4b94
title: INS-1E PBC

description: Untransfected INS-1E cells
description: <strong>Tilt Series Date:</strong> 2018-07-15</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rat INS-1E pancreatic beta cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Ray Stevens</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids, stimulated with glucose.</p>
<strong>Sample Preparation:</strong> Manual Blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-3/rawdata/</p>tomography3.mrc,  Tilt Series (Pixel Size 3.26 nm), 3.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-3/rawdata/tomography3.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography3.rec,  Reconstruction (Pixel Size 13.04 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-3/3dimage_117029/tomography3.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography3_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-3/3dimage_118523/tomography3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nxhnq-te508
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-7/rawdata/</p>tomo14.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-7/rawdata/tomo14.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo14_part59_12.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-7/3dimage_114913/tomo14_part59_12.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo14_part59_12.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-7/3dimage_114959/tomo14_part59_12.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/at5kw-jv918
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-17/rawdata/</p>tomo9.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-17/rawdata/tomo9.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo9_full.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-17/3dimage_114925/tomo9_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo9_full.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-17/3dimage_114971/tomo9_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rfmdm-2v559
title: INS-1E PBC

description: Untransfected INS-1E cells
description: <strong>Tilt Series Date:</strong> 2018-07-15</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rat INS-1E pancreatic beta cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Ray Stevens</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids, stimulated with glucose.</p>
<strong>Sample Preparation:</strong> Manual Blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-1/rawdata/</p>tomography1.mrc,  Tilt Series (Pixel Size 3.26 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-1/rawdata/tomography1.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography1_part110_14.rec,  Reconstruction (Pixel Size 13.04 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-1/3dimage_117027/tomography1_part110_14.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography1_full.rec,  Reconstruction (Pixel Size 0.0 nm), 3.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-1/3dimage_118511/tomography1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/31mk3-jbc07
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-62/rawdata/</p>Paeruginosa_dFliG27.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-62/rawdata/Paeruginosa_dFliG27.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG27_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-62/3dimage_104233/Paeruginosa_dFliG27_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG27.mrc.mdoc,  32.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-62/rawdata/Paeruginosa_dFliG27.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2f80c-nb252
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-45/rawdata/</p>Paeruginosa_dFliG10.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-45/rawdata/Paeruginosa_dFliG10.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG10_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-45/3dimage_104219/Paeruginosa_dFliG10_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG10.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-45/rawdata/Paeruginosa_dFliG10.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9yfej-1gg78
title: Nitrosocaldus yellowstonensis

description: Raw data files of Nitrosocaldus yellowstonensis
description: <strong>Tilt Series Date:</strong> 2019-07-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Candidatus Nitrosocaldus yellowstonensis</p>
<strong>Strain:</strong> HL72</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jose R De La Torre and students, SF state, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R0.6/1 copper 300 mesh, 10nm gold, 22deg C, 100% humidity, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-14/rawdata/</p>20190715_Ny_stationary_18.mrc,  Tilt Series (Pixel Size 0.2678 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-14/rawdata/20190715_Ny_stationary_18.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_18_full.rec,  Reconstruction (Pixel Size 1.0712 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-14/3dimage_105625/20190715_Ny_stationary_18_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_18.mrc.mdoc,  17.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-14/rawdata/20190715_Ny_stationary_18.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kmktb-yef11
title: Nitrosocaldus yellowstonensis

description: Raw data files of Nitrosocaldus yellowstonensis
description: <strong>Tilt Series Date:</strong> 2019-07-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Candidatus Nitrosocaldus yellowstonensis</p>
<strong>Strain:</strong> HL72</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jose R De La Torre and students, SF state, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R0.6/1 copper 300 mesh, 10nm gold, 22deg C, 100% humidity, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-5/3dimage_105632/</p>20190715_Ny_stationary_12_full.rec,  Reconstruction (Pixel Size 1.0712 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-5/3dimage_105632/20190715_Ny_stationary_12_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_12.mrc,  Tilt Series (Pixel Size 0.2678 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-5/rawdata/20190715_Ny_stationary_12.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_12.mrc.mdoc,  28.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-5/rawdata/20190715_Ny_stationary_12.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b65fy-x7048
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-49/rawdata/</p>Paeruginosa_dFliG14.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-49/rawdata/Paeruginosa_dFliG14.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG14_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-49/3dimage_104223/Paeruginosa_dFliG14_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG14.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-49/rawdata/Paeruginosa_dFliG14.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h0jcw-nxa18
title: Nitrosocaldus yellowstonensis

description: Raw data files of Nitrosocaldus yellowstonensis
description: <strong>Tilt Series Date:</strong> 2019-07-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Candidatus Nitrosocaldus yellowstonensis</p>
<strong>Strain:</strong> HL72</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jose R De La Torre and students, SF state, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R0.6/1 copper 300 mesh, 10nm gold, 22deg C, 100% humidity, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-2/3dimage_105623/</p>20190715_Ny_stationary_1_full.rec,  Reconstruction (Pixel Size 1.0712 nm), 737.3 MB  
        
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