DOI: 10.5061/dryad.rxwdbrvhk
title: Gene expression data from clones of FAO cells with different glucose production capability

description: In response to energy and nutrient shortage, the liver triggers several
 catabolic processes to promote survival. Despite recent progresses, the
 precise molecular mechanisms regulating the hepatic adaptation to fasting
 remain incompletely characterized. Here, we report the identification of
 Hydroxysteroid dehydrogenase-like 2 (HSDL2) as a mitochondrial protein
 highly induced by fasting. We show that activation of PGC1a-PPARa and
 inhibition of the PI3K- mTORC1 axis stimulate HSDL2 expression in
 hepatocytes. We found that HSDL2 depletion decreases cholesterol
 conversion to bile acids (BAs) and impairs FXR activity. HSDL2 knockdown
 also reduces mitochondrial respiration, fatty acid oxidation, and TCA
 cycle activity. Bioinformatics analyses revealed that hepatic Hsdl2
 expression positively associates with the postprandial excursion of
 various BA species in mice. Here, we show that liver-specific HSDL2
 depletion impacts BA metabolism and decreases circulating cholesterol
 levels upon refeeding. Overall, our report identifies HSDL2 as a
 fasting-induced mitochondrial protein that links nutritional signals to
 BAs and cholesterol homeostasis.
description: Single cells were isolated and amplified from a parental culture
 of FAO cells. The clones were expanded and glucose production was measured
 in each lines. Using this approach, we have identified Low and High
 glucose producers. We have then selected 6 Low and 6 High lines and we
 have performed microarrays to identify the genes differentially
 expressed.
description: # Gene expression data from clones of FAO cells with different glucose
 production capability
 [https://doi.org/10.5061/dryad.rxwdbrvhk](https://doi.org/10.5061/dryad.rxwdbrvhk) Here we provide microarray dataset from one experiment comparing FAO cells with either Low (n=6) or High (n=6) glucose prodcution capability. ## Description of the data and file structure FAO cells were cultured according to standard mammalian tissue culture protocols and sterile techniques. The cell lines were cultured in complete Dulbecco’s Modified Eagle Medium (DMEM) supplemented with Fetal Bovine Serum (FBS) (10%). Single cells were isolated and amplified from a parental culture of FAO cells. The clones were expanded and glucose production was measured in each lines. Using this approach, we have identified Low and High glucose producers. We have then selected 6 Low and 6 High lines and we have performed microarrays to identify the genes differentially expressed. Briefly, total mRNA was isolated from cells and tissues using the RNeasy Lipid Tissue Mini Kit. Biotinylated cRNA were prepared according to the standard Affymetrix WT PLUS Kit protocol. Following fragmentation, cRNA was hybridized for 16 hr at 45C on Rat Gene 2.0 ST. Chips were washed and stained on an Affymetrix Genechip Wash Station 450 using the FS450_0002 protocol. GeneChips were scanned using the Affymetrix GeneChip Scanner 3000. ## Code/Software The .cel files were proccessed using GeneChip Command Console Software (Thermo Fisher Scientific). Expression values were extracted using the Robust Multichip Average (RMA) method implemented in the oligo package in R.