DOI: 10.22002/gdq7d-frj18
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-5/3dimage_68947/</p>12jan14_C107_1_pNL_DN_AT1BT10002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        tubecone_VPS4BDNoverexp.tiff,  file associated to 3D Image #68947, 1.1 MB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_fullcone.tiff,  file associated to 3D Image #68947, 814.8 kB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_fullcone_f.tiff,  file associated to 3D Image #68947, 988.7 kB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_fullcone_e.tiff,  file associated to 3D Image #68947, 619.3 kB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_fullcone_d.tiff,  file associated to 3D Image #68947, 707.5 kB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_fullcone_c.tiff,  file associated to 3D Image #68947, 839.9 kB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_fullcone_b.tiff,  file associated to 3D Image #68947, 890.8 kB  
        
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        12jan14_C107_1_pNL_DN_AT1BT10002_full.tiff,  file associated to 3D Image #68947, 1.1 MB  
        
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