DOI: 10.5061/dryad.9w0vt4bts
title: Bioassay data of <em>Pentastiridius leporinus</em> on <em>Solanum bulbocastanum</em> and <em>Solanum tuberosum</em> ‘Gala’, with GC/MS analysis of plant volatiles and LC/MS metabolite profiling of exposed planthoppers

description: Data provided is associated with an article in the Journal of Applied
 Entomology on the lethal effects of the wild potato
 species Solanum bulbocastanum to a bacteria-transmitting
 insect vector (Pentastiridius leporinus) in potato and other crops. This
 data set includes data derived from two no-choice mortality trials and
 from choice trials with the insects on the wild potato vs. the cultivated
 potato. Raw data obtained from GC/MS analysis of collected volatile
 organic compounds of both plant species (wild potato and potato) are
 provided as well. Also, peak area values from LC/MS metabolite profiling
 of whole insects of the mortality trial, as well as insect gut extracts
 obtained from a plant sap feeding assay, are made available. 
description: **Title of the associated article** Lethal effects of the wild potato
 *Solanum bulbocastanum* on the planthopper *Pentastiridius leporinus*, a
 vector of bacterial pathogens in potato. Journal of Applied Entomology ---
 **Title of dataset** Bioassay data of *Pentastiridius leporinus* on
 *Solanum bulbocastanum* and *Solanum tuberosum* ‘Gala’, with GC/MS
 analysis of plant volatiles and LC/MS metabolite profiling of exposed
 planthoppers --- **Authors** Eva Therhaag^1,2^ (ORCID:
 0009-0001-9666-7269), Karin Gorzolka^3^ (ORCID: 0000-0002-1375-275X),
 Jürgen Gross^1,2 ♣^ (ORCID: 0000-0003-2614-9658) ^1^ Julius Kühn Institute
 (JKI) - Federal Research Centre for Cultivated Plants, Institute for Plant
 Protection in Fruit Crops and Viticulture, Schwabenheimer Str. 101, 69221
 Dossenheim, Germany ^2^ Hochschule Geisenheim University, Department of
 Crop Protection, Von-Lade-Str. 1, 65366 Geisenheim, Germany ^3^ Julius
 Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants,
 Institute for Ecological Chemistry, Plant Analysis and Stored Product
 Protection, Königin-Luise-Straße 19, 14195 Berlin, Germany ^♣^
 Corresponding author, email address:
 [juergen.gross@julius-kuehn.de](mailto:juergen.gross@julius-kuehn.de) ---
 **Data files** * coxph_data.xlsx: data of two no-choice mortality trials.
 Sheet "surv_exp1" contains data of the first mortality trial,
 sheet "surv_exp2" contains data of the second one. Dead
 specimens of adult *Pentastiridius leporinus* exposed to pots with
 *Solanum bulbocastanum* or to *Solanum tuberosum* cv ‘Gala’ were counted
 on a daily basis during the experiments; column descriptions as follows:
 ‘indiv’: dead individuals of *P. leporinus* in chronological order;
 ‘futime’: day of experiment in chronological order (exp1 started on 24
 June 2024; exp2 on 15 July 2024); ‘fustat’: 1 indicates that the
 individual was taking part in the study, whereas 0 indicates that it was
 censored; ‘rx’ indicates the treatment with 1 for *S. bulbocastanum* and 2
 for *S. tuberosum* cv ‘Gala’; ‘pot’ indicates the number of the pot. The
 column 'sex' contains f for female adults, and m for male adults
 of *P. leporinus*. The sheet "notes" contains column
 descriptions for ease of use. - R_Survival_analysis.txt: R code for the
 statistical survival analysis, with "coxph_data.xlsx" as data
 basis. * choice_wide.xlsx: count data of adults of *Pentastiridius
 leporinus* exposed to *Solanum tuberosum* cv ‘Gala’ and *Solanum
 bulbocastanum* in a dual-choice experiment (starting dates 5 & 10 July
 2024). Column descriptions are as follows:'cage': c1 to c6
 indicate cages of choice-experiment I and II (same
 cages);'batch': exp1= choice experiment I; exp2= choice
 experiment II;'timing’: t1 to t6 indicate observation timings of ½ h,
 1 h, 2 h, 3 h, 24 h and 48 h;'position_p1':indicated as
 'left' or 'right' of *S. bulbocastanum* plants, view
 from the entrance of the cage; 'p1': observed planthoppers on
 *S. bulbocastanum*; 'p2': observed planthoppers on *S.
 tuberosum* cv 'Gala'; 'elsewhere': number of
 non-decisive planthoppers at the respective observation timing (dead
 specimens excluded). Column descriptions are also provided in the sheet
 "notes" for ease of use. - R_Choice_betabinomial_glmm.txt: R
 code for statistical analysis of the two choice experiments, with
 "choice_wide.xlsx" as data basis. * GCMS_Therhaag_et_al.zip: raw
 data from GC/MS analysis of volatile organic compounds collected from six
 samples of *Solanum bulbocastanum* (et_014.raw to et_019.raw) and *Solanum
 tuberosum* cv ‘Gala’ (et_021.raw to et_026.raw) on 30 July 2024. This data
 type (.raw) is not proprietary and can be opened with AMDIS (Automated
 Mass Spectrometry Deconvolution and Identification System), a freely
 available software for GC-MS data interpretation developed by the National
 Institute of Standards and Technology (NIST), U.S. Department of Commerce.
 Annotated and identified compounds are provided in a supplement to the
 article. - data_voc.xlsx: sheet "compounds" lists peak area
 values of compounds obtained after GC/MS analysis of the volatile profiles
 of *Solanum bulbocastanum* and *Solanum tuberosum* cv
 'Gala'(data of "GCMS_Therhaag_et_al.zip"). Names
 (column 'compound') and retention indices (column 'RI) from
 the numbered compounds (column 'no') are listed in the sheet
 "table", additional information is provided in the supplement to
 the article. * R_VOC_analysis.txt: R code for statistical compositional
 analysis of the VOC profiles collected from *S.bulbocastanum* and *S.
 tuberosum* cv 'Gala' with "data_voc.xlsx" as data
 basis. - R codes were created with RStudio/2024.12.0+467 (“Kousa Dogwood”
 Release for windows, Posit Software, PBC) using R version 4.4.2 (R Core
 Team 2024. R: A Language and Environment for Statistical Computing. R
 Foundation for Statistical Computing, Vienna, Austria.
 [https://www.R-project.org/](https://www.R-project.org/)). *
 LCMS_data.xlsx: peak area values from LC/MS metabolite profiling of whole
 specimens of *Pentastiridius leporinus* of the second no-choice mortality
 trial; peak area values of LC/MS metabolite profiling of dissected gut
 extracts from individuals of *P. leporinus* of a plant sap uptake assay
 with twigs of *Solanum bulbocastanum* or *Solanum tuberosum* cv ‘Gala’.
 Tabs “Metadata P.l. whole insects” and “Metadata P.l. gut extracts”
 provide information on individual samples.