DOI: 10.5061/dryad.gc182
title: Data from: Mapping QTL contributing to variation in posterior lobe morphology between strains of Drosophila melanogaster

description: Closely-related, and otherwise morphologically similar insect species
 frequently show striking divergence in the shape and/or size of male
 genital structures, a phenomenon thought to be driven by sexual selection.
 Comparative interspecific studies can help elucidate the evolutionary
 forces acting on genital structures to drive this rapid differentiation.
 However, genetic dissection of sexual trait divergence between species is
 frequently hampered by the difficulty generating interspecific
 recombinants. Intraspecific variation can be leveraged to investigate the
 genetics of rapidly-evolving sexual traits, and here we carry out a
 genetic analysis of variation in the posterior lobe within D.
 melanogaster. The lobe is a male-specific process emerging from the
 genital arch of D. melanogaster and three closely-related species, is
 essential for copulation, and shows radical divergence in form across
 species. There is also abundant variation within species in the shape and
 size of the lobe, and while this variation is considerably more subtle
 than that seen among species, it nonetheless provides the raw material for
 QTL mapping. We created an advanced intercross population from a pair of
 phenotypically-different inbred strains, and after phenotyping and
 genotyping-by-sequencing the recombinants, mapped several QTL contributing
 to various measures of lobe morphology. The additional generations of
 crossing over in our mapping population led to QTL intervals that are
 smaller than is typical for an F2 mapping design. The intervals we map
 overlap with a pair of lobe QTL we previously identified in an independent
 mapping cross, potentially suggesting a level of shared genetic control of
 trait variation. Our QTL additionally implicate a suite of genes that have
 been shown to contribute to the development of the posterior lobe. These
 loci are strong candidates to harbor naturally-segregating sites
 contributing to phenotypic variation within D. melanogaster, and may also
 be those contributing to divergence in lobe morphology between species.
description: Description of raw files
 in data packageProvides information on
 all raw files in the data package, including sample
 IDs.FASTQ files for MSG
 samplesA gzipped tarball
 containing a directory holding 91 FASTQ files. Each file contains reads
 from a single genotyped individual, with genotyping carried out using
 Multiplexed Shotgun Genotyping (MSG).MergedMSGFASTQ.tar.gzFASTQ files for WGS samples (subset 1)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS01.tar.gzFASTQ files for WGS samples (subset 2)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS02.tar.gzFASTQ files for WGS samples (subset 3)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS03.tar.gzFASTQ files for WGS samples (subset 4)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS04.tar.gzFASTQ files for WGS samples (subset 5)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS05.tar.gzFASTQ files for WGS samples (subset 6)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS06.tar.gzFASTQ files for WGS samples (subset 7)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS07.tar.gzFASTQ files for WGS samples (subset 8)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS08.tar.gzFASTQ files for WGS samples (subset 9)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS09.tar.gzFASTQ files for WGS samples (subset 10)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS10.tar.gzFASTQ files for WGS samples (subset 11)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS11.tar.gzFASTQ files for WGS samples (subset 12)A gzipped tarball containing a directory holding multiple FASTQ files. Each file contains reads from a single genotyped individual, with genotyping carried out using low pass Whole Genome Sequencing (WGS).WGS12.tar.gz