cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14268697
title: Prospects for using gamification in Industry 4.0

description: Abstract Paper aims This study aims to analyze the main applications of gamification in the industry, identifying its benefits and challenges, and to understand how game techniques are impacting the formation of engineers. Finally, the main perspectives of the use of gamification in the Industry 4.0 are presented. Originality The scientific literature was analyzed to infer practical applications, challenges, benefits and perspectives that can guide the implementation of gamification in Industry 4.0, aiming to help professionals and researchers to take better advantage of this approach and propel this new phase of the industry. Research method A literature review was conducted on the use of gamification in the industry. Main findings In this article, eleven aspects that must be taken into consideration during the application of gamification in the industries were observed. Five perspectives/situations of Gamification use were raised, namely: in the servitization; in learning and training; as a tool to increase intrinsic motivation; introducing new technologies and products; and innovation and flexibility. Implications for theory and practice This study systematically brings together the main approaches to gamification and its applications in the current industry scenario. In addition, this work contributes to the process of designing and implementing gamification in the industry.
DOI: 10.6084/m9.figshare.14268700
title: Additive Manufactured Nanocomposites for Bone Tissue Engineering Applications: an Overview

description: Tissue Engineering aims to repair, regenerate or restore damaged tissues. Structures known as scaffolds can be manufactured in different ways and with various materials such as synthetic and natural polymers as well as inorganic materials. Additive Manufacturing (AM) has been used to produce porous scaffolds. Particularly, Selective Laser Sintering (SLS) produces materials and composites through a selective solidification of a variety of fine powders. AM-made biomaterials may be suitable for different affected or injured parts by stimulating the biological system around the implant. The present work aims to review relevant concepts concerning the nanostructure for regeneration of the bone tissue when in contact with the native tissue, as well as the suitable techniques and materials to fabricate it.
DOI: 10.6084/m9.figshare.14268700.v1
title: Additive Manufactured Nanocomposites for Bone Tissue Engineering Applications: an Overview

description: Tissue Engineering aims to repair, regenerate or restore damaged tissues. Structures known as scaffolds can be manufactured in different ways and with various materials such as synthetic and natural polymers as well as inorganic materials. Additive Manufacturing (AM) has been used to produce porous scaffolds. Particularly, Selective Laser Sintering (SLS) produces materials and composites through a selective solidification of a variety of fine powders. AM-made biomaterials may be suitable for different affected or injured parts by stimulating the biological system around the implant. The present work aims to review relevant concepts concerning the nanostructure for regeneration of the bone tissue when in contact with the native tissue, as well as the suitable techniques and materials to fabricate it.
DOI: 10.6084/m9.figshare.14268705
title: Multi-categorical classification using deep learning applied to the diagnosis of gastric cancer

description: ABSTRACT Introduction: Pathologists currently face a substantial increase in workload and complexity of their diagnosis work on different types of cancer. This is due to the increased incidence and detection of neoplasms, associated with diagnostic subspecialization and the advent of personalized medicine. There are numerous treatments available for different types of cancer, and the diagnosis must be dispensed quickly and accurately for each case. Deep learning is a tool that has been used in daily life, including image detection, and there is growing interest in its application in Medicine and especially in Pathology, where it has a revolutionary potential. Objective: In this article, we present deep learning, in particular convolutional neural networks, as a potential technique for the analysis of digitized images of histopathological slides, detecting identifiable patterns in an automated manner, introducing the possibility of applying this technology as an auxiliary tool in the diagnosis of neoplasms, especially in gastric cancer, the object of this preliminary study. Method: From a database of digitized images of histopathological slides representative of gastric cancer, we identified three morphological patterns of neoplasia, as well as non-neoplastic tissue patterns, with which we train a convolutional neural network algorithm, designed to identify and categorize similar images within these standards, in an automated manner. Results: The results of identification and automatic classification in the defined categories were satisfactory, with ROC curves above 0.9. Conclusion: The results show the potential application of convolutional neural networks for digitized slides of gastric cancer, in accordance with international literature findings.
DOI: 10.6084/m9.figshare.14268706
title: Disseminated and cutaneous cryptococcosis by C. neoformans VNI in an immunocompetent patient

description: ABSTRACT Cryptococcosis is caused by yeasts of the Cryptococcus neoformans/C. gattii complex, presenting cutaneous, respiratory and disseminated forms. A 44-year-old immunocompetent male with facial lesion and latent pneumonia was hospitalized and misdiagnosed with paracoccidioidomycosis. Computerized tomography scans showed pulmonary and neurological involvement, and cultures/China ink, cryptococcal antigen test and restriction fragment length polymorphism of urease gene (URA5-RFLP) confirmed C. neoformans genotype VNI. Hemoculture indicated ampicillin-resistant Klebsiella pneumoniae (healthcare-associated infection). Fluconazole was administered, but after resistance detection, amphotericin B was chosen (cumulative dose/1500 mg). The patient was discharged with clinical remission (75 days) and amphotericin for one year (maintenance phase).
DOI: 10.6084/m9.figshare.14268706.v1
title: Disseminated and cutaneous cryptococcosis by C. neoformans VNI in an immunocompetent patient

description: ABSTRACT Cryptococcosis is caused by yeasts of the Cryptococcus neoformans/C. gattii complex, presenting cutaneous, respiratory and disseminated forms. A 44-year-old immunocompetent male with facial lesion and latent pneumonia was hospitalized and misdiagnosed with paracoccidioidomycosis. Computerized tomography scans showed pulmonary and neurological involvement, and cultures/China ink, cryptococcal antigen test and restriction fragment length polymorphism of urease gene (URA5-RFLP) confirmed C. neoformans genotype VNI. Hemoculture indicated ampicillin-resistant Klebsiella pneumoniae (healthcare-associated infection). Fluconazole was administered, but after resistance detection, amphotericin B was chosen (cumulative dose/1500 mg). The patient was discharged with clinical remission (75 days) and amphotericin for one year (maintenance phase).
DOI: 10.6084/m9.figshare.14268720
title: Ductal carcinoma in situ of the breast: correlation of architectural, cytological, IHC findings and recurrence analysis

description: ABSTRACT Objective: This study evaluated the histopathological features of ductal carcinoma in situ (DCIS), including cytological grade, architectural pattern and immunohistochemistry (IHC) in pure DCIS and DCIS associated with invasive carcinoma of no special type (ICNST). Methods: We evaluated a series of 232 cases of pure DCIS and DCIS associated with ICNST from a total of 399 breast carcinomas from a population consisting by women diagnosed with breast cancer and submitted to breast surgery from 2011 to 2015. Results: DCIS presented a mixed architectural pattern in most cases (56%); the solid subtype was the most common morphology (30%). High-grade DCIS was identified in 84/221 cases (38%), and comedonecrosis was present in 106/221 cases (48%). High-grade was more common in the solid subtype (61/155 cases, 39%, p &lt; 0.001). Tumor size was greater in the presence of comedonecrosis than in the absence (mean 27 vs 20 mm, p = 0.009). Estrogen receptor (ER) was positive in 81% of cases with a cribriform pattern (p = 0.013). Greater locoregional recurrence was found in the comedonecrosis (15%) and micropapillary (19%) subtypes in DCIS associated with ICNST. Conclusion: We observed a greater relationship of ER with the low nuclear grade, while Ki-67 was related to the high-grade. DCIS presented a higher nuclear grade compared to ICNST. The less common pure pattern was the micropapillary, and the most common, the solid. Comedonecrosis was more frequent in the solid pattern. Our results showed that high-grade was more common in the solid and comedo subtype, and low-grade was more frequent in the cribriform.
DOI: 10.6084/m9.figshare.14268720.v1
title: Ductal carcinoma in situ of the breast: correlation of architectural, cytological, IHC findings and recurrence analysis

description: ABSTRACT Objective: This study evaluated the histopathological features of ductal carcinoma in situ (DCIS), including cytological grade, architectural pattern and immunohistochemistry (IHC) in pure DCIS and DCIS associated with invasive carcinoma of no special type (ICNST). Methods: We evaluated a series of 232 cases of pure DCIS and DCIS associated with ICNST from a total of 399 breast carcinomas from a population consisting by women diagnosed with breast cancer and submitted to breast surgery from 2011 to 2015. Results: DCIS presented a mixed architectural pattern in most cases (56%); the solid subtype was the most common morphology (30%). High-grade DCIS was identified in 84/221 cases (38%), and comedonecrosis was present in 106/221 cases (48%). High-grade was more common in the solid subtype (61/155 cases, 39%, p &lt; 0.001). Tumor size was greater in the presence of comedonecrosis than in the absence (mean 27 vs 20 mm, p = 0.009). Estrogen receptor (ER) was positive in 81% of cases with a cribriform pattern (p = 0.013). Greater locoregional recurrence was found in the comedonecrosis (15%) and micropapillary (19%) subtypes in DCIS associated with ICNST. Conclusion: We observed a greater relationship of ER with the low nuclear grade, while Ki-67 was related to the high-grade. DCIS presented a higher nuclear grade compared to ICNST. The less common pure pattern was the micropapillary, and the most common, the solid. Comedonecrosis was more frequent in the solid pattern. Our results showed that high-grade was more common in the solid and comedo subtype, and low-grade was more frequent in the cribriform.
DOI: 10.6084/m9.figshare.14268724
title: Osmolyte accumulation and antioxidant metabolism during germination of vigorous maize seeds subjected to water deficit

description: ABSTRACT. The objective of this work was to evaluate the alterations of antioxidant enzyme reserves and antioxidant enzymes during germination under water deficit in maize hybrids and to associate with seed vigor, determining the mechanisms related to tolerance for this stress. Two three-way maize hybrids were characterized by their vigor at different levels of water deficit induced by polyethylene glycol 6000. Next, the seeds were hydrated at different osmotic potentials (0.0, -0.3, and -0.9 MPa) and removed at different times to assess the levels of the total soluble protein, total soluble sugars, proline, starch, and antioxidant enzymes, such as superoxide dismutase, catalase and ascorbate peroxidase. The analysis of variance, Tukey test at 5% and principal component analysis (PCA) were used. The vigorous hybrid (HT1) was more efficient than the low vigor hybrid seeds (HT2) in mobilizing the total soluble protein during the initial stages of germination and the total soluble sugars before and after root protrusion under water deficit in addition to increasing the catalase activity at the different osmotic potentials that were assessed.
DOI: 10.6084/m9.figshare.14268724.v1
title: Osmolyte accumulation and antioxidant metabolism during germination of vigorous maize seeds subjected to water deficit

description: ABSTRACT. The objective of this work was to evaluate the alterations of antioxidant enzyme reserves and antioxidant enzymes during germination under water deficit in maize hybrids and to associate with seed vigor, determining the mechanisms related to tolerance for this stress. Two three-way maize hybrids were characterized by their vigor at different levels of water deficit induced by polyethylene glycol 6000. Next, the seeds were hydrated at different osmotic potentials (0.0, -0.3, and -0.9 MPa) and removed at different times to assess the levels of the total soluble protein, total soluble sugars, proline, starch, and antioxidant enzymes, such as superoxide dismutase, catalase and ascorbate peroxidase. The analysis of variance, Tukey test at 5% and principal component analysis (PCA) were used. The vigorous hybrid (HT1) was more efficient than the low vigor hybrid seeds (HT2) in mobilizing the total soluble protein during the initial stages of germination and the total soluble sugars before and after root protrusion under water deficit in addition to increasing the catalase activity at the different osmotic potentials that were assessed.
DOI: 10.6084/m9.figshare.14268731
title: Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E 2 and leukotriene B 4 signaling in apical periodontitis

description: Abstract Purpose To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak’s test, and qRT-PCR data using two-way ANOVA followed by Tukey’s test (α=0.05). Results Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E 2 and leukotriene B 4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4.
DOI: 10.6084/m9.figshare.14268731.v1
title: Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E 2 and leukotriene B 4 signaling in apical periodontitis

description: Abstract Purpose To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak’s test, and qRT-PCR data using two-way ANOVA followed by Tukey’s test (α=0.05). Results Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E 2 and leukotriene B 4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4.
DOI: 10.6084/m9.figshare.5671018
title: Embryonic dormancy in seeds of Bactris gasipaes Kunth (peach-palm)

description: Abstract: Bactris gasipaes is a domesticated palm whose fruits are of great importance for the Amazonian people and whose heart of palm is also receiving economic interest in other brazilian and Latin America regions. The aim of this study was verify embryonic dormancy and its correlation with first cataphyll emergence in B. gasipaes seeds collected from four plants at Manaus city and four others at Coari city, both in the Amazonas state, Brazil. After extraction and cleaning, some of the seeds (4 replications of 25 per plant) were sown in a seedbed with a sawdust and sand mixture as substrate, and embryos (4 replications of 10 per plant), after extraction, were inoculated into half strength Murashige and Skoog cultures. Were used 100 seeds and 40 embryo per treatment. Whole seed and embryo germination varied between the different source plants and locations, with the greatest difference observed for the emergence of first cataphyll from seeds in the seedbed. For the most part of variables, results of seed and embryo were positively associated, namely, as one went up the other also, and vice versa. These results suggesting that, at least in part, seed dormancy in Bactris gasipaes is associated with embryonic dormancy.
DOI: 10.6084/m9.figshare.11965740
title: Rural context and professional rehabilitation in a region of the Ribeira Valley

description: ABSTRACT Aim: To investigate the sociodemographic profile, of work and health, of workers from a region of Registro (SP), locate in Ribeira Valley, and to identify the barriers and facilitators in the professional rehabilitation process of the National Institute of Social Security (INSS), of insured persons workers of this region. Methods: Two steps: (1) A list of workers was obtained from the Family Health Strategy of rural neighborhoods in the city of Registro (SP), and a questionnaire with sociodemographic data, of work and health, was applied, under the form of an interview. (2) Subsequently, two reference professionals of the INSS, who worked at the headquarters of this region were identified and, in addition, individual interviews were conducted to analyze thematic content. Results: Regarding the profile of the subjects, most workers worked on banana plantations, lived on farms, had low schooling; all reported musculoskeletal symptoms, among others. In interviews with reference professionals of the INSS, it was possible to observe, among the main barriers found to professional rehabilitation: the profile of insured rural people with low schooling, with difficulties in transportation, few courses offered, and the evaluation of the medical expert based on the biomedical model, with suffering and injustice. Among the facilitators: the welcome of the social workers reference professionals, the bond established and the empathy. Conclusion: There is a need for public policies that can guarantee the rights of workers and promote improvements in the current model of professional rehabilitation.
DOI: 10.6084/m9.figshare.11965740.v1
title: Rural context and professional rehabilitation in a region of the Ribeira Valley

description: ABSTRACT Aim: To investigate the sociodemographic profile, of work and health, of workers from a region of Registro (SP), locate in Ribeira Valley, and to identify the barriers and facilitators in the professional rehabilitation process of the National Institute of Social Security (INSS), of insured persons workers of this region. Methods: Two steps: (1) A list of workers was obtained from the Family Health Strategy of rural neighborhoods in the city of Registro (SP), and a questionnaire with sociodemographic data, of work and health, was applied, under the form of an interview. (2) Subsequently, two reference professionals of the INSS, who worked at the headquarters of this region were identified and, in addition, individual interviews were conducted to analyze thematic content. Results: Regarding the profile of the subjects, most workers worked on banana plantations, lived on farms, had low schooling; all reported musculoskeletal symptoms, among others. In interviews with reference professionals of the INSS, it was possible to observe, among the main barriers found to professional rehabilitation: the profile of insured rural people with low schooling, with difficulties in transportation, few courses offered, and the evaluation of the medical expert based on the biomedical model, with suffering and injustice. Among the facilitators: the welcome of the social workers reference professionals, the bond established and the empathy. Conclusion: There is a need for public policies that can guarantee the rights of workers and promote improvements in the current model of professional rehabilitation.