DOI: 10.5061/dryad.z612jm6hd
title: 3-(4-Hydroxy-3-methoxyphenyl) propionic acid contributes to improved hepatic lipid metabolism via GPR41

description: 3-(4-hydroxy-3-methoxyphenyl) propionic acid (HMPA) is a metabolite
 produced by the gut microbiota through the conversion of
 4-hydroxy-3-methoxycinnamic acid (HMCA), which is a widely distributed
 hydroxycinnamic acid-derived metabolite found abundantly in plants.
 Several beneficial effects of HMPA have been suggested, such as
 antidiabetic properties, anticancer activities, and cognitive function
 improvement, in animal models and human studies. However, the intricate
 molecular mechanisms underlying the bioaccessibility and bioavailability
 profile following HMPA intake and the substantial modulation of metabolic
 homeostasis by HMPA require further elucidation. In this study, we
 effectively identified and characterized HMPA-specific GPR41 receptor,
 with greater affinity than HMCA. The activation of this receptor plays a
 crucial role in the anti-obesity effects and improvement of hepatic
 steatosis by stimulating the lipid catabolism pathway. For the improvement
 of metabolic disorders, our results provide insights into the development
 of functional foods, including HMPA, and preventive pharmaceuticals
 targeting GPR41.
description: RNA was extracted from the liver of HMPA (2.0 g/kg bodyweight)-treated mice using an RNAiso Plus reagent (Takara Bio)
 and RNeasy mini kit (Qiagen, Germany). RNA-seq libraries were generated with the TruSeq
 Stranded mRNA Library Prep Kit (Illumina, CA, USA) and sequenced on NovaSeq 6000. Approximately
 4-Gb paired-end reads of 100-bp length per sample were obtained.
 RNA-seq data were pre-processed using Trimmomatic to
 remove adapters or poor-quality reads, and trimmed sequences
 were assessed using FastQC. The reads were aligned to
 the Illumina iGenomes NCBI GRCm38. To obtain DEGs from all
 comparisons, raw read
 counts were subjected to relative log expression
 normalization.
 Data expressed as fold
 change of DEGs were identified based on the following criteria: false
 discovery rate (FDR)-adjusted <em>p</em>-value &lt; 0.05
 (using the Benjamini–Hochberg procedure) and |log2 (fold change)|
 &gt; 0.5. A gene set
 enrichment analysis was
 performed using Bioconductor version 3.0.
description: # 3-(4-Hydroxy-3-methoxyphenyl) propionic acid contributes to improved
 hepatic lipid metabolism via GPR41
 [https://doi.org/10.5061/dryad.z612jm6hd](https://doi.org/10.5061/dryad.z612jm6hd) RNA was extracted from the liver of HMPA (2.0 g/kg bodyweight)-treated mice using an RNAiso Plus reagent (Takara Bio) and RNeasy mini kit (Qiagen, Germany). RNA-seq libraries were generated with the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA) and sequenced on NovaSeq 6000.Approximately 4-Gb paired-end reads of 100-bp length per sample were obtained. ## Description of the data and file structure **Liver from HMPA-administered mice (n = 4 per group)** 【Vehicle control group (n = 4)】 ●TR\_2358\_001 TR\_2358\_001\_1.fastq.gz TR\_2358\_001\_2.fastq.gz ●TR\_2358\_002 TR\_2358\_002\_1.fastq.gz TR\_2358\_002\_2.fastq.gz ●TR\_2358\_003 TR\_2358\_003\_1.fastq.gz TR\_2358\_003\_2.fastq.gz ●TR\_2358\_004 TR\_2358\_004\_1.fastq.gz TR\_2358\_004\_2.fastq.gz 【HMPA group (n = 4)】 ●TR\_2358\_006 TR\_2358\_006\_1.fastq.gz TR\_2358\_006\_2.fastq.gz ●TR\_2358\_007 TR\_2358\_007\_1.fastq.gz TR\_2358\_007\_2.fastq.gz ●TR\_2358\_009 TR\_2358\_009\_1.fastq.gz TR\_2358\_009\_2.fastq.gz ●TR\_2358\_010 TR\_2358\_010\_1.fastq.gz TR\_2358\_010\_2.fastq.gz