cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20343570
title: Historical and modern distribution data of the yellow fever mosquito Aedes aegypti in the western Palaearctic region

description: Data set encompassing published historical (&lt;1980) and modern (&gt;=1980) distribution data of the invasive yellow fever mosquito <em>Aedes aepgyti </em>in the western palaearctic region, with all original references. Historical European distribution data collection was obtained through an online literature search in Ovid MEDLINE®, CAB direct, and Web of Science, without year and language restrictions applied and, mainly, by reference tracking. Modern mosquito distribution data have been collected routinely within the VectorNet project via online systematic literature search, reference tracking, and grey literature and unpublished dataset sharing. For both the historical and modern periods, mosquito introductions without evidence of establishment were excluded from the dataset. Modern records which could not be substantiated following information exchanges with the authors or with local colleagues were also excluded (Algeria, Lebanon).
DOI: 10.6084/m9.figshare.20343570.v1
title: Historical and modern distribution data of the yellow fever mosquito Aedes aegypti in the western Palaearctic region

description: Data set encompassing published historical (&lt;1980) and modern (&gt;=1980) distribution data of the invasive yellow fever mosquito <em>Aedes aepgyti </em>in the western palaearctic region, with all original references. Historical European distribution data collection was obtained through an online literature search in Ovid MEDLINE®, CAB direct, and Web of Science, without year and language restrictions applied and, mainly, by reference tracking. Modern mosquito distribution data have been collected routinely within the VectorNet project via online systematic literature search, reference tracking, and grey literature and unpublished dataset sharing. For both the historical and modern periods, mosquito introductions without evidence of establishment were excluded from the dataset. Modern records which could not be substantiated following information exchanges with the authors or with local colleagues were also excluded (Algeria, Lebanon).
DOI: 10.6084/m9.figshare.19165280.v6
title: mb.final.repeat-sort-mask.fa

description: mb-genome
DOI: 10.6084/m9.figshare.19214253
title: An analysis of the novice motorcyclist crashes in Taiwan

description: Motorcycles comprised over 60% of motor vehicles in Taiwan. There were still many motorcycle crashes in Taiwan, especially among young riders. This study investigated the characteristics of novice motorcyclist crashes in Taiwan over the period January 2011 to December 2016. Various risk factors affecting the severity of novice motorcyclist crashes, such as the rider characteristics, licensing conditions, and the environment, were examined. To model the count data with multiple crash severities, several regression models were considered. The multinomial logit (MNL) model, ordered logit (OL) model, and partial proportional odds (PPO) model were chosen and investigated for the relationships between the severity of novice motorcyclist crashes and potential risk factors. The results showed that the novice rider who was underage or unlicensed had a higher probability of a fatal crash. Male sex, helmet use, drinking, college student, frontal impact, urban or dry road, and daytime all played significant roles in novice motorcyclist crashes. Taiwan traffic safety needs further policy adjustments and public education toward novice motorcycle crashes. Adequate driving training and providing a user-friendly environment for novice riders could help. Taiwan should consider graduated driver licensing systems for skill-building and riding supervision for new motorcyclists.
DOI: 10.6084/m9.figshare.19214253.v1
title: An analysis of the novice motorcyclist crashes in Taiwan

description: Motorcycles comprised over 60% of motor vehicles in Taiwan. There were still many motorcycle crashes in Taiwan, especially among young riders. This study investigated the characteristics of novice motorcyclist crashes in Taiwan over the period January 2011 to December 2016. Various risk factors affecting the severity of novice motorcyclist crashes, such as the rider characteristics, licensing conditions, and the environment, were examined. To model the count data with multiple crash severities, several regression models were considered. The multinomial logit (MNL) model, ordered logit (OL) model, and partial proportional odds (PPO) model were chosen and investigated for the relationships between the severity of novice motorcyclist crashes and potential risk factors. The results showed that the novice rider who was underage or unlicensed had a higher probability of a fatal crash. Male sex, helmet use, drinking, college student, frontal impact, urban or dry road, and daytime all played significant roles in novice motorcyclist crashes. Taiwan traffic safety needs further policy adjustments and public education toward novice motorcycle crashes. Adequate driving training and providing a user-friendly environment for novice riders could help. Taiwan should consider graduated driver licensing systems for skill-building and riding supervision for new motorcyclists.
DOI: 10.6084/m9.figshare.19214247
title: Study on the influence of seating orientation during frontal impacts by child occupant human body model response analysis

description: Autonomous driving cars must be developed to ensure that children will have the highest level of protection in case of collision. Changes to the vehicle cabin design (different seat orientations, fully reclining seats, etc.) may significantly impact child occupant safety. Understanding child occupant responses under these new conditions is necessary to decrease risk and enhance child safety. In this study, child occupant response in different seating orientations exposed to frontal impacts with a focus on the head injuries and kinematics was analyzed. Finite elements simulations were performed using the PIPER 6-year-old human body model (HBM). All simulations were carried out in a generic full vehicle environment. The child model was positioned in an adequate generic car restraint system (CRS) in the left rear vehicle seat in 4 seating orientations: 0° (forward-facing position), 30°, 60°, and 90° (living room position). Two scenarios were evaluated for all seating orientations according to the left front seat backrest position: reclined position nominal upright and rest position (55°). All seat configurations were subjected to the mobile progressive deformable barrier frontal impact (European New Car Assessment Programme [Euro NCAP] frontal impact testing protocol). A total of 8 scenarios were simulated in LS-DYNA. Based on the Euro NCAP injury risk rate, 90° seating orientation (living room position) was the safest among all selected scenarios independent of the left front seat backrest position. The worst case was found in 60° seat rotation. The highest values for Head Injury Criterion (HIC) and head acceleration (Acc 3 ms) were noted for this case. Higher Brain Injury Criterion (BrIC) values were observed at higher seat rotation angles. Hence, a 90° seating orientation showed the highest BrIC value. Attending to the skull stress, greater head injuries were caused principally by contact with the vehicle interior (seat headrest). Maximum stress values were reached at 30° and 60° seating orientations with the front seat in rest position. In 90° seating orientation, high stress values were also identified. These results show that attending to these new seating orientations, current child safety standards are not sufficient to ensure children the highest level of protection. Other additional criteria such as BrIC or skull stress that offer a way to capture brain injuries should be used.
DOI: 10.6084/m9.figshare.19214247.v1
title: Study on the influence of seating orientation during frontal impacts by child occupant human body model response analysis

description: Autonomous driving cars must be developed to ensure that children will have the highest level of protection in case of collision. Changes to the vehicle cabin design (different seat orientations, fully reclining seats, etc.) may significantly impact child occupant safety. Understanding child occupant responses under these new conditions is necessary to decrease risk and enhance child safety. In this study, child occupant response in different seating orientations exposed to frontal impacts with a focus on the head injuries and kinematics was analyzed. Finite elements simulations were performed using the PIPER 6-year-old human body model (HBM). All simulations were carried out in a generic full vehicle environment. The child model was positioned in an adequate generic car restraint system (CRS) in the left rear vehicle seat in 4 seating orientations: 0° (forward-facing position), 30°, 60°, and 90° (living room position). Two scenarios were evaluated for all seating orientations according to the left front seat backrest position: reclined position nominal upright and rest position (55°). All seat configurations were subjected to the mobile progressive deformable barrier frontal impact (European New Car Assessment Programme [Euro NCAP] frontal impact testing protocol). A total of 8 scenarios were simulated in LS-DYNA. Based on the Euro NCAP injury risk rate, 90° seating orientation (living room position) was the safest among all selected scenarios independent of the left front seat backrest position. The worst case was found in 60° seat rotation. The highest values for Head Injury Criterion (HIC) and head acceleration (Acc 3 ms) were noted for this case. Higher Brain Injury Criterion (BrIC) values were observed at higher seat rotation angles. Hence, a 90° seating orientation showed the highest BrIC value. Attending to the skull stress, greater head injuries were caused principally by contact with the vehicle interior (seat headrest). Maximum stress values were reached at 30° and 60° seating orientations with the front seat in rest position. In 90° seating orientation, high stress values were also identified. These results show that attending to these new seating orientations, current child safety standards are not sufficient to ensure children the highest level of protection. Other additional criteria such as BrIC or skull stress that offer a way to capture brain injuries should be used.
DOI: 10.6084/m9.figshare.11316569.v2
title: Phloroglucinol derivatives from <i>Hypericum</i> species induce <i>in vitro</i> proliferation of cells involved in the wound healing process

description: The genus <i>Hypericum</i> (Hypericaceae) is a recognized source of therapeutic agents, being some species widely used due to their wound healing properties. In a previous study, south Brazilian species <i>H. caprifoliatum, H. carinatum, H. connatum, H. myrianthum</i> and <i>H. polyanthemum</i> demonstrated potential to induce proliferation of keratinocytes. In the present study, the effect of phloroglucinol derivatives isolated from <i>Hypericum</i> on cell proliferation of human keratinocytes, fibroblasts and stem cells was investigated. The best results, determined by the MTT assay, were achieved with cariphenone B at concentrations of 0.01 and 0.1 µM (122.3% and 114%, respectively) on HaCaT cells. Uliginosin B was able to induce the proliferation of mesenchymal stem cells (129% at 10 µM) and MRC5 fibroblasts (152.5% at 5 µM). These findings confirm the capacity of phloroglucinol derivatives to induce the <i>in vitro</i> cellular proliferation and reinforce the importance of <i>Hypericum</i> species as potential sources of wound healing compounds.
DOI: 10.6084/m9.figshare.11316569
title: Phloroglucinol derivatives from <i>Hypericum</i> species induce <i>in vitro</i> proliferation of cells involved in the wound healing process

description: The genus <i>Hypericum</i> (Hypericaceae) is a recognized source of therapeutic agents, being some species widely used due to their wound healing properties. In a previous study, south Brazilian species <i>H. caprifoliatum, H. carinatum, H. connatum, H. myrianthum</i> and <i>H. polyanthemum</i> demonstrated potential to induce proliferation of keratinocytes. In the present study, the effect of phloroglucinol derivatives isolated from <i>Hypericum</i> on cell proliferation of human keratinocytes, fibroblasts and stem cells was investigated. The best results, determined by the MTT assay, were achieved with cariphenone B at concentrations of 0.01 and 0.1 µM (122.3% and 114%, respectively) on HaCaT cells. Uliginosin B was able to induce the proliferation of mesenchymal stem cells (129% at 10 µM) and MRC5 fibroblasts (152.5% at 5 µM). These findings confirm the capacity of phloroglucinol derivatives to induce the <i>in vitro</i> cellular proliferation and reinforce the importance of <i>Hypericum</i> species as potential sources of wound healing compounds.
DOI: 10.6084/m9.figshare.11316569.v1
title: Phloroglucinol derivatives from <i>Hypericum</i> species induce <i>in vitro</i> proliferation of cells involved in the wound healing process

description: The genus <i>Hypericum</i> (Hypericaceae) is a recognized source of therapeutic agents, being some species widely used due to their wound healing properties. In a previous study, south Brazilian species <i>H. caprifoliatum, H. carinatum, H. connatum, H. myrianthum</i> and <i>H. polyanthemum</i> demonstrated potential to induce proliferation of keratinocytes. In the present study, the effect of phloroglucinol derivatives isolated from <i>Hypericum</i> on cell proliferation of human keratinocytes, fibroblasts and stem cells was investigated. The best results, determined by the MTT assay, were achieved with cariphenone B at concentrations of 0.01 and 0.1 µM (122.3% and 114%, respectively) on HaCaT cells. Uliginosin B was able to induce the proliferation of mesenchymal stem cells (129% at 10 µM) and MRC5 fibroblasts (152.5% at 5 µM). These findings confirm the capacity of phloroglucinol derivatives to induce the <i>in vitro</i> cellular proliferation and reinforce the importance of <i>Hypericum</i> species as potential sources of wound healing compounds.
DOI: 10.6084/m9.figshare.13089213
title: Catechin from <i>Burkea africana</i> Hook. Exhibits <i>in vitro</i> inhibition of human telomerase activity

description: There has been an increasing interest in natural products with the ability to inhibit telomerase activity in tumour and cancerous cells. Green tea catechins have been reported previously to inhibit telomerase, but it was unknown whether catechins from other plant sources could exhibit this property. We isolated 2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5,7-triol (catechin without the presence of a galloyl unit) from the stem bark of <i>B. africana</i>, and tested its ability to inhibit recombinant, partially purified telomerase produced in rabbit reticulocyte lysates. The <i>B. africana</i> catechin inhibited the telomere extension activity of telomerase with an IC<sub>50</sub> of approximately 4.7 µg/ml. This finding indicates that the galloyl unit may not be solely responsible for the inhibition of telomerase activity by catechins. This is the first report of the telomerase-inhibiting potential of catechin from the stem bark of <i>B. africana.</i>
DOI: 10.6084/m9.figshare.13089213.v1
title: Catechin from <i>Burkea africana</i> Hook. Exhibits <i>in vitro</i> inhibition of human telomerase activity

description: There has been an increasing interest in natural products with the ability to inhibit telomerase activity in tumour and cancerous cells. Green tea catechins have been reported previously to inhibit telomerase, but it was unknown whether catechins from other plant sources could exhibit this property. We isolated 2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5,7-triol (catechin without the presence of a galloyl unit) from the stem bark of <i>B. africana</i>, and tested its ability to inhibit recombinant, partially purified telomerase produced in rabbit reticulocyte lysates. The <i>B. africana</i> catechin inhibited the telomere extension activity of telomerase with an IC<sub>50</sub> of approximately 4.7 µg/ml. This finding indicates that the galloyl unit may not be solely responsible for the inhibition of telomerase activity by catechins. This is the first report of the telomerase-inhibiting potential of catechin from the stem bark of <i>B. africana.</i>
DOI: 10.6084/m9.figshare.12849308
title: <i>In silico</i> pharmacological prediction and cytotoxicity of flavonoids glycosides identified by UPLC-DAD-ESI-MS/MS in extracts of <i>Humulus lupulus</i> leaves cultivated in Brazil

description: Ethanolic (EB) extract and hexanic (SH) and hydromethanolic (SEM) sub-extracts of <i>Humulus lupulus</i> leaves were submitted to cytotoxicity evaluation and to phytochemical methods. The effect of EB and SEM on cellular cycle was evaluated by propidium iodide method and the phases were quantified through flow cytometry. The cytotoxicity assessment was done using T24 and MRC5 cells, with EB and SEM (25–1200 µg/mL). By means of UPLC-DAD-MS/MS data were identified the flavonoids astragaline, nicotiflorin, kaempferol-7-<i>O</i>-rutinoside, robinin, hyperin, rutin, quercetin-7-<i>O</i>-rutinoside and manghaslin. EB (800 µg/mL) and SEM (1200 µg/mL) reduces the T24 cell viability. These extracts at 25 µg/mL stimulate the growth of MRC5 cells, evidencing a selective cytotoxicity. After 24 h of the treatment with extracts was not observed cycle arrest of T24 cells. The bioactivity prediction of the flavonoids was evaluated <i>in silico</i> through <i>in house</i> Active-IT software and PASSonline which indicated potential activity as antitumoral, cytotoxic, anti-inflammatory, antiparasitic, antimicrobial, antiviral and others.
DOI: 10.6084/m9.figshare.12849308.v1
title: <i>In silico</i> pharmacological prediction and cytotoxicity of flavonoids glycosides identified by UPLC-DAD-ESI-MS/MS in extracts of <i>Humulus lupulus</i> leaves cultivated in Brazil

description: Ethanolic (EB) extract and hexanic (SH) and hydromethanolic (SEM) sub-extracts of <i>Humulus lupulus</i> leaves were submitted to cytotoxicity evaluation and to phytochemical methods. The effect of EB and SEM on cellular cycle was evaluated by propidium iodide method and the phases were quantified through flow cytometry. The cytotoxicity assessment was done using T24 and MRC5 cells, with EB and SEM (25–1200 µg/mL). By means of UPLC-DAD-MS/MS data were identified the flavonoids astragaline, nicotiflorin, kaempferol-7-<i>O</i>-rutinoside, robinin, hyperin, rutin, quercetin-7-<i>O</i>-rutinoside and manghaslin. EB (800 µg/mL) and SEM (1200 µg/mL) reduces the T24 cell viability. These extracts at 25 µg/mL stimulate the growth of MRC5 cells, evidencing a selective cytotoxicity. After 24 h of the treatment with extracts was not observed cycle arrest of T24 cells. The bioactivity prediction of the flavonoids was evaluated <i>in silico</i> through <i>in house</i> Active-IT software and PASSonline which indicated potential activity as antitumoral, cytotoxic, anti-inflammatory, antiparasitic, antimicrobial, antiviral and others.
DOI: 10.6084/m9.figshare.13353719
title: Budget impact analysis of pneumococcal conjugate vaccines in Colombia

description: <b>Objective:</b> Pneumococcal diseases including invasive pneumococcal disease (IPD), pneumonia, and acute otitis media (AOM) impose a substantial public health burden. This study performed a budget impact analysis of the use of pneumococcal conjugate vaccines (PCVs) in the National Immunization Program (NIP) in Colombia. <b>Methods:</b> We compared the direct medical cost of the scenario without and with PCV vaccination using either pneumococcal non-typeable <i>Haemophilus influenzae</i> protein D conjugate vaccine (PHiD-CV) or 13-valent pneumococcal conjugate vaccine (PCV-13) over 5 years (2020–2024) from the health-care system perspective. Vaccine efficacy estimates were obtained from published sources and vaccine prices were taken from the Pan-American Health Organization Revolving Fund. Vaccine coverage was assumed to be 90% based on Colombia data. <b>Results:</b> Using PHiD-CV in the NIP in Colombia would reduce the estimated cost for treating pneumococcal disease by US$46.1 m over the 2020–2024 period (US$40.2 m using PCV-13), with a budget impact of US$100.1 m for PHiD-CV (US$121.4 m for PCV-13), and would cost US$3.1 m less per year on vaccine doses than using PCV-13. <b>Conclusion:</b> These findings are potentially valuable for the selection of vaccines for their national immunization programs under conditions of budgetary constraint.
DOI: 10.6084/m9.figshare.13353719.v1
title: Budget impact analysis of pneumococcal conjugate vaccines in Colombia

description: <b>Objective:</b> Pneumococcal diseases including invasive pneumococcal disease (IPD), pneumonia, and acute otitis media (AOM) impose a substantial public health burden. This study performed a budget impact analysis of the use of pneumococcal conjugate vaccines (PCVs) in the National Immunization Program (NIP) in Colombia. <b>Methods:</b> We compared the direct medical cost of the scenario without and with PCV vaccination using either pneumococcal non-typeable <i>Haemophilus influenzae</i> protein D conjugate vaccine (PHiD-CV) or 13-valent pneumococcal conjugate vaccine (PCV-13) over 5 years (2020–2024) from the health-care system perspective. Vaccine efficacy estimates were obtained from published sources and vaccine prices were taken from the Pan-American Health Organization Revolving Fund. Vaccine coverage was assumed to be 90% based on Colombia data. <b>Results:</b> Using PHiD-CV in the NIP in Colombia would reduce the estimated cost for treating pneumococcal disease by US$46.1 m over the 2020–2024 period (US$40.2 m using PCV-13), with a budget impact of US$100.1 m for PHiD-CV (US$121.4 m for PCV-13), and would cost US$3.1 m less per year on vaccine doses than using PCV-13. <b>Conclusion:</b> These findings are potentially valuable for the selection of vaccines for their national immunization programs under conditions of budgetary constraint.
DOI: 10.6084/m9.figshare.20071892.v1
title: Supplementary Material for: Differential Gene Expression in Cord Blood of Infants Diagnosed with Cerebral Palsy: a Pilot Analysis of the BEAM Cohort

description: The Beneficial Effects of Antenatal Magnesium (BEAM) clinical trial was conducted between 1997-2007, and demonstrated a significant reduction in cerebral palsy (CP) in preterm infants who were exposed to peripartum magnesium sulfate (MgSO4). However, the mechanism by which MgSO4 confers neuroprotection remains incompletely understood. Cord blood samples from this study were interrogated during an era when next generation sequencing was not widely accessible and few gene expression differences or biomarkers were identified between treatment groups. Our goal was to use bulk RNA deep sequencing to identify differentially expressed genes comparing the following four groups: newborns who ultimately developed CP treated with MgSO4 or placebo, and controls (newborns who ultimately did not develop CP) treated with MgSO4 or placebo. Those who died after birth were excluded. We found that MgSO4 upregulated expression of SCN5A only in the control group, with no change in gene expression in cord blood of newborns who ultimately developed CP. Regardless of MgSO4 exposure, expression of NPBWR1 and FTO were upregulated in cord blood of newborns who ultimately developed CP compared with controls. These data support that MgSO4 may not exert its neuroprotective effect through changes in gene expression. Moreover, NPBWR1 and FTO may be useful as biomarkers, and may suggest new mechanistic pathways to pursue in understanding the pathogenesis of CP. The small number of cases ultimately available for this secondary analysis, with male predominance and mild CP phenotype, is a limitation of the study. In addition, differentially expressed genes were not validated by qRT-PCR.