cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10025633
title: Effects of Physical Training on the Myocardium of Oxariectomized LDLr Knockout Mice: MMP 2/9, Collagen I/III, Inflammation and Oxidative Stress

description: Abstract Background: The emergence of coronary heart disease is increased with menopause, physical inactivity and with dyslipidemia. Physical training is known to promote the improvement of cardiovascular functions. Objective: To investigate the effects of aerobic physical training on the left ventricle in ovariectomized LDL knockout mice. Methods: Thirty animals were divided into 6 groups (n = 5): Sedentary non-ovariectomized control; Sedentary ovariectomized control; Trained ovariectomized control; Sedentary non-ovariectomized LDL-knockout, sedentary ovariectomized LDL-knockout and trained ovariectomized LDL-knockout. We analyzed the average parameters of apparent density of collagen fibers types I and III, and metalloproteinase type 2 and type 9, were considered significant p &lt; 0.05. Results: The results showed that the proposed exercise protocol altered the volume of type I collagen fibers, altered collagen remodeling parameters (MMP-2), and also reduced the 8-hydroxy-2’-deoxyguanosine (8OHdG) oxidative stress parameter. Conclusion: Moderate intensity aerobic training acts on collagen fiber volume, on collagen remodeling with the reduction of oxidative stress in the left ventricles of ovariectomized LDL-knockout mice.
DOI: 10.6084/m9.figshare.10025636
title: Quality indicators in nutrition therapy within the intensive care setting of a Brazilian teaching hospital

description: Abstract: The topic addressed by this study was quality indicators in nutritional therapy. The aim was to evaluate the quality of nutritional therapy in an intensive care unit for adults in a Brazilian university hospital. Ten quality indicators selected from a list prepared by the Clinical Nutrition Task Force of the International Life Sciences Institute (ILSI-Brazil) were applied. It was observed that the use of quality indicators of nutritional therapy allowed the identification of compliance and nonconformity processes, which still need additional adjustments.
DOI: 10.6084/m9.figshare.10025627
title: Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity

description: Phosphoenolpyruvate carboxykinase (PEPCK) is a ubiquitous enzyme found in all known groups of organisms, acting in the reversible conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) in the presence of divalent metal ion, and dependent of adenosine 5’-triphosphate (ATP) or guanosine-5’-triphosphate (GTP). PEPCK is an important enzyme in the metabolism of some organisms, such as Trypanosoma cruzi, being suggested as a potential drug target to treat Chagas’ disease. Its catalytic activity is, classically, measured by coupled assays. Herein, a direct assay by liquid chromatography-tandem mass spectrometry (LC-MS/MS) capable of quantifying PEP, in co-elution with OAA by the differentiation obtained by the mass spectra, is reported. The developed assay was used throughout the purification protocol in order to measure the activity of PEPCK of T. cruzi, which was expressed in Escherichia coli. The purified enzyme was kinetically characterized by the developed method with Michaelis-Menten constant (KMapp) values of 96 ± 4 and 275 ± 18 µmol L-1 to OAA and ATP as substrates, respectively. The developed assay was also used for ligand screening and proved to be able to identify very low inhibitions for small molecules (50 µmol L-1).
DOI: 10.6084/m9.figshare.10025630
title: Exudate - phenolphthalein pH test for evaluation of validity in seeds of Libidibia ferrea

description: Abstract: Seed companies are looking for promising, quick and effective alternatives to determine the physiological quality of seeds. The objective of the current work was to study the efficiency of the exudate - phenolphthalein pH test to evaluate the seeds of two lots of Libidibia ferrea (Mart. ex Tul.) L. P. Queiroz var. ferrea. The statistical design for the the exudate - phenolphthalein pH test was completely randomized with four replicates of 50 seeds in a factorial design (2 x 5), two seed lots and five soaking periods (30, 60, 90, 120, and 150 minutes), respectively, using two constant temperatures (25 and 30°C). The percentage of viability and germination of the seeds did not differ in the temperatures of 25 and 30°C and in the soaking periods by the exudate - phenolphthalein pH test. Thus, it is recommended that the test be conducted for at least 30 minutes in distilled and deionized water at the constant temperature of 25 or 30°C to evaluate the vigor of the Libidibia ferrea.
DOI: 10.6084/m9.figshare.10025627.v1
title: Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity

description: Phosphoenolpyruvate carboxykinase (PEPCK) is a ubiquitous enzyme found in all known groups of organisms, acting in the reversible conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) in the presence of divalent metal ion, and dependent of adenosine 5’-triphosphate (ATP) or guanosine-5’-triphosphate (GTP). PEPCK is an important enzyme in the metabolism of some organisms, such as Trypanosoma cruzi, being suggested as a potential drug target to treat Chagas’ disease. Its catalytic activity is, classically, measured by coupled assays. Herein, a direct assay by liquid chromatography-tandem mass spectrometry (LC-MS/MS) capable of quantifying PEP, in co-elution with OAA by the differentiation obtained by the mass spectra, is reported. The developed assay was used throughout the purification protocol in order to measure the activity of PEPCK of T. cruzi, which was expressed in Escherichia coli. The purified enzyme was kinetically characterized by the developed method with Michaelis-Menten constant (KMapp) values of 96 ± 4 and 275 ± 18 µmol L-1 to OAA and ATP as substrates, respectively. The developed assay was also used for ligand screening and proved to be able to identify very low inhibitions for small molecules (50 µmol L-1).
DOI: 10.6084/m9.figshare.10025633.v1
title: Effects of Physical Training on the Myocardium of Oxariectomized LDLr Knockout Mice: MMP 2/9, Collagen I/III, Inflammation and Oxidative Stress

description: Abstract Background: The emergence of coronary heart disease is increased with menopause, physical inactivity and with dyslipidemia. Physical training is known to promote the improvement of cardiovascular functions. Objective: To investigate the effects of aerobic physical training on the left ventricle in ovariectomized LDL knockout mice. Methods: Thirty animals were divided into 6 groups (n = 5): Sedentary non-ovariectomized control; Sedentary ovariectomized control; Trained ovariectomized control; Sedentary non-ovariectomized LDL-knockout, sedentary ovariectomized LDL-knockout and trained ovariectomized LDL-knockout. We analyzed the average parameters of apparent density of collagen fibers types I and III, and metalloproteinase type 2 and type 9, were considered significant p &lt; 0.05. Results: The results showed that the proposed exercise protocol altered the volume of type I collagen fibers, altered collagen remodeling parameters (MMP-2), and also reduced the 8-hydroxy-2’-deoxyguanosine (8OHdG) oxidative stress parameter. Conclusion: Moderate intensity aerobic training acts on collagen fiber volume, on collagen remodeling with the reduction of oxidative stress in the left ventricles of ovariectomized LDL-knockout mice.
DOI: 10.6084/m9.figshare.10025630.v1
title: Exudate - phenolphthalein pH test for evaluation of validity in seeds of Libidibia ferrea

description: Abstract: Seed companies are looking for promising, quick and effective alternatives to determine the physiological quality of seeds. The objective of the current work was to study the efficiency of the exudate - phenolphthalein pH test to evaluate the seeds of two lots of Libidibia ferrea (Mart. ex Tul.) L. P. Queiroz var. ferrea. The statistical design for the the exudate - phenolphthalein pH test was completely randomized with four replicates of 50 seeds in a factorial design (2 x 5), two seed lots and five soaking periods (30, 60, 90, 120, and 150 minutes), respectively, using two constant temperatures (25 and 30°C). The percentage of viability and germination of the seeds did not differ in the temperatures of 25 and 30°C and in the soaking periods by the exudate - phenolphthalein pH test. Thus, it is recommended that the test be conducted for at least 30 minutes in distilled and deionized water at the constant temperature of 25 or 30°C to evaluate the vigor of the Libidibia ferrea.
DOI: 10.6084/m9.figshare.10025636.v1
title: Quality indicators in nutrition therapy within the intensive care setting of a Brazilian teaching hospital

description: Abstract: The topic addressed by this study was quality indicators in nutritional therapy. The aim was to evaluate the quality of nutritional therapy in an intensive care unit for adults in a Brazilian university hospital. Ten quality indicators selected from a list prepared by the Clinical Nutrition Task Force of the International Life Sciences Institute (ILSI-Brazil) were applied. It was observed that the use of quality indicators of nutritional therapy allowed the identification of compliance and nonconformity processes, which still need additional adjustments.
DOI: 10.6084/m9.figshare.10025618
title: Risk management during construction of electric power substations

description: Abstract The construction of electric power substations poses a great number of risks due to its dangerous environment. The objective of this work was to evaluate the perception of construction workers on risks, methods they use for evaluation and managing risks to which they are exposed. This study carried out a qualitative and quantitative analysis during the expansion of one electric substation, adding an additional 100 MVA power transformer. The risks were identified and analysed by using the Preliminary Risk Analysis (PRA). Additionally, a questionnaire on construction safety was created and applied on all 27 workers which participated in the construction. The results showed which activities are present during the construction of a power substation, which risks are involved and how to manage them. It was found that among several, the PRA and Checklists were the most commonly used analysis tools. Further on, it was found a relation between training and risk perception. Among perceived risks were: noise, working at heights, electric shocks, traffic accidents, poisonous animals, dust, fire and explosions. The conclusion of this study was that the construction of electric power substations brings several safety risks and that the instruction of workers allows an effective risk management, and can reduce the number of accidents in this type of working activity.
DOI: 10.6084/m9.figshare.10025621
title: Evaluation of Lipid Profiles of Children and Youth from Basic Health Units in Campinas, SP, Brazil: A Cross-Sectional Laboratory Study

description: Abstract Background: Among dyslipidemias, hypercholesterolemia is considered the main risk factor for cardiovascular diseases in adults. In childhood and adolescence, elevated total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) are positively associated with atherosclerosis markers, however, systematic screening for dyslipidemias in these groups is a controversial topic. Objective: To characterize the frequencies, types and severity of dyslipidemias in children and adolescents attended at the Basic Health Units managed by SUS in Campinas/SP. Methods: After an agreement with the Municipal Health Department of Campinas, consecutive results of serum lipid profiles (n = 312,650) of individuals of both sexes (n = 62,530) aged between 1 day old and 19 years were obtained, from 2008 to 2015. Age groups and dyslipidemias were classified according to recommendations in the literature. The statistical significance level adopted was the probability value (p) of 0.05 or less. Results: The observed frequencies of increased TC, triglycerides (TG), LDL-C and non-HDL-C (NHDL-C) were 33%, 40%, 29% and 13% respectively, and of reduced high-density lipoprotein cholesterol (HDL-C) the frequency was 39%. The frequencies, in general, were greater in females and in the southwest and south regions of the city, whose populations are more vulnerable from the socioeconomic point of view; on the other hand, in children and adolescents, the frequencies of TG and HDL-C prevailed, respectively. Conclusions: The high frequency and regionalization of dyslipidemias in children and adolescents indicate the need for specific actions in the handling and treatment of such diseases by the public health system of Campinas.
DOI: 10.6084/m9.figshare.10025615
title: Biochemical and Molecular Mechanisms of Glucose Uptake Stimulated by Physical Exercise in Insulin Resistance State: Role of Inflammation

description: Abstract Obesity associated with systemic inflammation induces insulin resistance (IR), with consequent chronic hyperglycemia. A series of reactions are involved in this process, including increased release of proinflammatory cytokines, and activation of c-Jun N-terminal kinase (JNK), nuclear factor-kappa B (NF-κB) and toll-like receptor 4 (TLR4) receptors. Among the therapeutic tools available nowadays, physical exercise (PE) has a known hypoglycemic effect explained by complex molecular mechanisms, including an increase in insulin receptor phosphorylation, in AMP-activated protein kinase (AMPK) activity, in the Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) pathway, with subsequent activation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), Rac1, TBC1 domain family member 1 and 4 (TBC1D1 and TBC1D4), in addition to a variety of signaling molecules, such as GTPases, Rab and soluble N-ethylmaleimide-sensitive factor attached protein receptor (SNARE) proteins. These pathways promote greater translocation of GLUT4 and consequent glucose uptake by the skeletal muscle. Phosphoinositide-dependent kinase (PDK), atypical protein kinase C (aPKC) and some of its isoforms, such as PKC-iota/lambda also seem to play a fundamental role in the transport of glucose. In this sense, the association between autophagy and exercise has also demonstrated a relevant role in the uptake of muscle glucose. Insulin, in turn, uses a phosphoinositide 3-kinase (PI3K)-dependent mechanism, while exercise signal may be triggered by the release of calcium from the sarcoplasmic reticulum. The objective of this review is to describe the main molecular mechanisms of IR and the relationship between PE and glucose uptake.
DOI: 10.14272/addocblmgwtaed-uhfffaoysa-n/chmo0000630
title: infrared absorption spectroscopy (IR) ()
title: InChI=1S/C42H52O4/c1-25(2)39(43)33-17-9-29(10-18-33)37(30-11-19-34(20-12-30)40(44)26(3)4)38(31-13-21-35(22-14-31)41(45)27(5)6)32-15-23-36(24-16-32)42(46)28(7)8/h9-28,39-46H,1-8H3

description: dataset for infrared absorption spectroscopy (IR) IR (ATR, ṽ) = 3548 (w), 3483 (w), 3386 (w), 3024 (w), 2958 (s), 2928 (w), 2870 (m), 1506 (w), 1468 (w), 1409 (w), 1383 (w), 1364 (w), 1312 (w), 1261 (w), 1171 (w), 1112 (w), 1096 (w), 1081 (w), 1014 (vs), 980 (w), 936 (m), 871 (w), 829 (vs), 800 (s), 789 (m), 771 (vs), 754 (w), 737 (w), 667 (w), 643 (w), 629 (w), 588 (m), 562 (m), 550 (m), 528 (m), 523 (m), 504 (m), 482 (m), 473 (m), 465 (w), 448 (w), 439 (w), 429 (w), 418 (m), 412 (m), 402 (m), 395 (m), 375 (m) cm–1.
DOI: 10.6084/m9.figshare.10025618.v1
title: Risk management during construction of electric power substations

description: Abstract The construction of electric power substations poses a great number of risks due to its dangerous environment. The objective of this work was to evaluate the perception of construction workers on risks, methods they use for evaluation and managing risks to which they are exposed. This study carried out a qualitative and quantitative analysis during the expansion of one electric substation, adding an additional 100 MVA power transformer. The risks were identified and analysed by using the Preliminary Risk Analysis (PRA). Additionally, a questionnaire on construction safety was created and applied on all 27 workers which participated in the construction. The results showed which activities are present during the construction of a power substation, which risks are involved and how to manage them. It was found that among several, the PRA and Checklists were the most commonly used analysis tools. Further on, it was found a relation between training and risk perception. Among perceived risks were: noise, working at heights, electric shocks, traffic accidents, poisonous animals, dust, fire and explosions. The conclusion of this study was that the construction of electric power substations brings several safety risks and that the instruction of workers allows an effective risk management, and can reduce the number of accidents in this type of working activity.
DOI: 10.6084/m9.figshare.10025621.v1
title: Evaluation of Lipid Profiles of Children and Youth from Basic Health Units in Campinas, SP, Brazil: A Cross-Sectional Laboratory Study

description: Abstract Background: Among dyslipidemias, hypercholesterolemia is considered the main risk factor for cardiovascular diseases in adults. In childhood and adolescence, elevated total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) are positively associated with atherosclerosis markers, however, systematic screening for dyslipidemias in these groups is a controversial topic. Objective: To characterize the frequencies, types and severity of dyslipidemias in children and adolescents attended at the Basic Health Units managed by SUS in Campinas/SP. Methods: After an agreement with the Municipal Health Department of Campinas, consecutive results of serum lipid profiles (n = 312,650) of individuals of both sexes (n = 62,530) aged between 1 day old and 19 years were obtained, from 2008 to 2015. Age groups and dyslipidemias were classified according to recommendations in the literature. The statistical significance level adopted was the probability value (p) of 0.05 or less. Results: The observed frequencies of increased TC, triglycerides (TG), LDL-C and non-HDL-C (NHDL-C) were 33%, 40%, 29% and 13% respectively, and of reduced high-density lipoprotein cholesterol (HDL-C) the frequency was 39%. The frequencies, in general, were greater in females and in the southwest and south regions of the city, whose populations are more vulnerable from the socioeconomic point of view; on the other hand, in children and adolescents, the frequencies of TG and HDL-C prevailed, respectively. Conclusions: The high frequency and regionalization of dyslipidemias in children and adolescents indicate the need for specific actions in the handling and treatment of such diseases by the public health system of Campinas.
DOI: 10.14272/addocblmgwtaed-uhfffaoysa-n/chmo0000595
title: 13C nuclear magnetic resonance spectroscopy (13C NMR) ()
title: InChI=1S/C42H52O4/c1-25(2)39(43)33-17-9-29(10-18-33)37(30-11-19-34(20-12-30)40(44)26(3)4)38(31-13-21-35(22-14-31)41(45)27(5)6)32-15-23-36(24-16-32)42(46)28(7)8/h9-28,39-46H,1-8H3

description: dataset for 13C nuclear magnetic resonance spectroscopy (13C NMR) 13C NMR (100 MHz, DMSO-d6 [39.5 ppm], ppm) δ = 143.1 (4C, Cq), 141.7 (4C, Cq), 140.2 (2C, Cq), 130.0 (8C, Car.H), 125.7 (8C, CarH), 77.3 (4C, CHOH), 35.0 (4C, CH(CH3)2), 18.9 (4C, CH3), 18.0 (4C, CH3).
DOI: 10.6084/m9.figshare.10025603
title: Agreement between two Meal Quality Indexes adapted to evaluate food consumption of institutionalized elderly

description: ABSTRACT Objective To assess agreement between two meal quality indexes adapted for elderly people, evidencing their similarities and differences Methods Agreement study with 322 institutionalized elderly individuals. Food consumption data were collected by the method of weighted food record, in two nonconsecutive days. The Meal Quality Index and the Main Meal Quality Index of each older adult were calculated for the lunch meal. Agreement between methods was tested by cross-classification in quartiles and weighted kappa (Kw), and the difference between medians by the Wilcoxon test. Results The Meal Quality Index median was 54.67 points and the Main Meal Quality Index 53.51 points (p=0.723). When the components of each index were assessed, those associated to the consumption of carbohydrates, total fat and saturated fat were similar. The consumption of fruits separated from vegetables in the Main MealQuality Index evidenced low consumption of vegetables by the elderly. Cross-classification by quartiles showed good agreement; the exact one being 48.8% and the disagreement 3.4% (Kw=0,447). The proportion of elderlyindividuals in the same exact or adjacent quartile was greater than 85.0%. Agreement was higher in males (89.4%, Kw=0.475); in the age range of 70 to 79 years (91.1%; Kw=0.562) and in non-profit nursing homes(96.7%; Kw=0.622). Conclusion The two indexes reviewed show a good agreement between them and common characteristics. The number of components is higher in the MMQI and may represent a more detailed assessment of meal quality.
DOI: 10.6084/m9.figshare.10025606
title: A conceptual framework for defense logistics

description: Abstract Defense logistics (DL) is an important and yet unexplored knowledge area of Production Engineering. Therefore, the purpose of this paper is to establish the DL problem domain. The focus is on industrial, technological, institutional, organizational and, specially, strategic management aspects applied to logistics in the defense sector. It also proposes a conceptual framework identifying DL objectives, functional areas and interfaces with the environment. The framework defines the Defense Logistics Base (DLB) as a system meant to create and sustain military capability, but also involved with the development of industrial capability, related to high and medium-high technologies, applied to high value products, with dual use. Furthermore, it suggests a research agenda for future work on strategic management related to DL.
DOI: 10.6084/m9.figshare.10025615.v1
title: Biochemical and Molecular Mechanisms of Glucose Uptake Stimulated by Physical Exercise in Insulin Resistance State: Role of Inflammation

description: Abstract Obesity associated with systemic inflammation induces insulin resistance (IR), with consequent chronic hyperglycemia. A series of reactions are involved in this process, including increased release of proinflammatory cytokines, and activation of c-Jun N-terminal kinase (JNK), nuclear factor-kappa B (NF-κB) and toll-like receptor 4 (TLR4) receptors. Among the therapeutic tools available nowadays, physical exercise (PE) has a known hypoglycemic effect explained by complex molecular mechanisms, including an increase in insulin receptor phosphorylation, in AMP-activated protein kinase (AMPK) activity, in the Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) pathway, with subsequent activation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), Rac1, TBC1 domain family member 1 and 4 (TBC1D1 and TBC1D4), in addition to a variety of signaling molecules, such as GTPases, Rab and soluble N-ethylmaleimide-sensitive factor attached protein receptor (SNARE) proteins. These pathways promote greater translocation of GLUT4 and consequent glucose uptake by the skeletal muscle. Phosphoinositide-dependent kinase (PDK), atypical protein kinase C (aPKC) and some of its isoforms, such as PKC-iota/lambda also seem to play a fundamental role in the transport of glucose. In this sense, the association between autophagy and exercise has also demonstrated a relevant role in the uptake of muscle glucose. Insulin, in turn, uses a phosphoinositide 3-kinase (PI3K)-dependent mechanism, while exercise signal may be triggered by the release of calcium from the sarcoplasmic reticulum. The objective of this review is to describe the main molecular mechanisms of IR and the relationship between PE and glucose uptake.