DOI: 10.5061/dryad.2dj50
title: Data from: Antennal transcriptome analysis and expression profiles of odorant binding proteins in Eogystia hippophaecolus (Lepidoptera: Cossidae)

description: Background: Eogystia hippophaecolus (Hua et al.) (Lepidoptera: Cossidae)
 is the major threat to seabuckthorn plantations in China. Specific and
 highly efficient artificial sex pheromone traps was developed and used to
 control it. However, the molecular basis for the pheromone recognition is
 not known. So we established the antennal transcriptome of E.
 hippophaecolus and characterized the expression profiles of odorant
 binding proteins. These results establish and improve the basis knowledge
 of the olfactory receptive system, furthermore provide a theoretical basis
 for the development of new pest control method. Results: We identified 29
 transcripts encoding putative odorant-binding proteins (OBPs), 18 putative
 chemosensory proteins (CSPs), 63 odorant receptors (ORs), 13 gustatory
 receptors (GRs), 12 ionotropic receptors (IRs), and two sensory neuron
 membrane proteins (SNMPs). Based on phylogenetic analysis, we found one
 Orco and three pheromone receptors of E. hippophaecolus and found that
 EhipGR13 detects sugar, EhipGR11 and EhipGR3 detect bitter. Nine OBPs
 expression profile indicated that most were the highest expression in
 antennae, consistent with functions of OBPs in binding and transporting
 odors during the antennal recognition process. OBP6 was external expressed
 in male genital-biased in, and this locus may be responsible for pheromone
 binding and recognition as well as mating. OBP1 was the highest and biased
 expressed in the foot and may function as identification of host plant
 volatiles. Conclusions: One hundred thirty-seven chemosensory proteins
 were identified and the accurate functions and groups of part proteins
 were obtained by phylogenetic analysis. The most OBPs were antenna-biased
 expressed, which are involved in antennal recognition. However, few OBP
 was detected biased expression in the foot and external genitalia, and
 these loci may function in pheromone recognition, mating, and the
 recognition of plant volatiles.
description: Figure 3
 Neighbor-joining phylogenetic tree of chemosensory proteins
 (CSPs)Figure 3
 Neighbor-joining phylogenetic tree of chemosensory proteins (CSPs) The NJ
 phylogenetic analysis of CSPs of E. hippophaecolus (EhipCSP, red) was
 performed with reference CSPs of D. melanogaster (DmelCSP, Diptera, blue)
 and CSPs of Lepidoptera species (black). The stability of the nodes was
 assessed by bootstrap analysis with 1,000 replications, and only bootstrap
 values ≥0.6 are shown at the corresponding nodes. The scale bar represents
 0.5 substitutions per site.Figure 4
 Neighbor-joining phylogenetic tree of sensory neuron membrane proteins
 (SNMPs)Figure 4
 Neighbor-joining phylogenetic tree of sensory neuron membrane proteins
 (SNMPs) The NJ phylogenetic analysis of SNMPs of E. hippophaecolus
 (EhipSNMP, red) was performed with reference SNMPs of D. ponderosae
 (DponSNMP, purple), I. typographus (ItypSNMP, purple), T. molitor
 (TmolSNMP, purple), T. castaneum (TcasSNMP, purple), D. melanogaster
 (DmelSNMP, Diptera, blue), B. mori (BmorSNMP, Lepidoptera, dark),
 H.armigera (HarmSNMP, dark),and A. mellifera (AmelSNMP, Hymenoptera,
 green). The stability of the nodes was assessed by bootstrap analysis with
 1,000 replications, and only bootstrap values ≥0.6 are shown at the
 corresponding nodes. The scale bar represents 0.04 substitutions per
 site.Figure4 Neighbor-joining
 phylogenetic tree of sensory neuron membrane protein
 (SNMPs).jpgFigure 7
 Neighbor-joining phylogenetic tree of gustatory receptors
 (GRs)Figure 7
 Neighbor-joining phylogenetic tree of gustatory receptors (GRs) The NJ
 phylogenetic analysis of GRs of E. hippophaecolus (EhipGR, red) was
 performed with reference GRs of B.mori (BmorGR, dark), H.armigera (HarmGR,
 dark)[85], A. mellifera (AmelGR, Hymenoptera, green), T. castaneum
 (TcasGR, Coleoptera, purple) and D. melanogaster (DmelGR, Diptera, blue).
 The GRs group labelled with red circle refers to detect CO2. sugar and
 bitter. The stability of the nodes was assessed by bootstrap analysis with
 1,000 replications, and only bootstrap values ≥0.6 are shown at the
 corresponding nodes. The scale bar represents 0.25 substitutions per
 site.Figure 5
 Neighbor-joining phylogenetic tree of odorant receptors
 (ORs)The NJ phylogenetic
 analysis of ORs of E. hippophaecolus (EhipOR, red) was performed with
 reference ORs of D. melanogaster (DmelOR, Diptera, blue) and ORs of
 Lepidoptera species (black). The red circles refer to Orco and PR lineage.
 The stability of the nodes was assessed by bootstrap analysis with 1,000
 replications, and only bootstrap values ≥0.6 are shown at the
 corresponding nodes. The scale bar represents 0.5 substitutions per
 site.Figure5 Neighbor-joining
 phylogenetic tree of odorant receptors
 (ORs).jpgFigure 6
 Neighbor-joining phylogenetic tree of ionotropic receptors
 (IRs)The NJ phylogenetic
 analysis of IRs of E. hippophaecolus (EhipIR, red) was performed with
 reference IRs of H.armigera (HarmIR, black), D. melanogaster (DmelIR,
 Diptera, blue) and IRs of other Lepidoptera species (black). The IRs
 groups labelled with red circle were reference of Van Schooten[82]. The
 stability of the nodes was assessed by bootstrap analysis with 1,000
 replications, and only bootstrap values ≥0.6 are shown at the
 corresponding nodes. The scale bar represents 0.5 substitutions per
 site.Figure
 6 Neighbor-joining phylogenetic tree of ionotropic receptors
 (IRs).jpgFigure 2
 Neighbor-joining phylogenetic tree of odorant-binding proteins
 (OBPs)The NJ phylogenetic
 analysis of OBPs of E. hippophaecolus (EhipOBP, red) was performed with
 reference OBPs of D. melanogaster (DmelOBP, Diptera, blue) and OBPs of
 Lepidoptera species (black)[79]. The red circles refer to PBP/GOBP
 lineage. Pale yellow sector refer to the Lepidoptera-specific lineages.
 The stability of the nodes was assessed by bootstrap analysis with 1,000
 replications, and only bootstrap values ≥ 0.6 are shown at the
 corresponding nodes. The scale bar represents 05 substitutions per
 site.