cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.353
title: DNA METHYLATION OF GENES RELATED TO HONEY BEE WORKER REPRODUCTIVE PHYSIOLOGY

description: Venom gland and ovary are the important organs related to a reproductive system in honey bee workers. The aim of this study is to investigate whether DNA methylation is involved in the regulation of function role of both organs in workers. In the first part, the frequency of DNA methylation is determined in four Thai native honey bee species which are A. andreniformis, A. florea, A. cerana indica and A. dorsata by focusing on venom phospholipase A2 (PLA2). Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that the highest expression of PLA2 is found in house bees of all four Apis spp. while the high expression of PLA2 is observed in foragers of A. florea and A. dorsata. In contrast, expression of PLA2 is undetectable in pupae from all Apis spp. The PLA2 activity and specific activity from crude venom extract shows the higher level in house bees than in black-eyed pupae in all studied bees. DNA methylation level of pupae is higher than that of house bees and foragers of A. florea and A. dorsata but it is not the case in A. andreniformis and A. cerana indica. In the second part, under the queenless condition, workers are divided into control and experimental (CO2 narcosis) cages. Using methylation-sensitive amplified fragment length polymorphism (MS-AFLP) assay, there is no difference detectable in a pattern of genome-wide DNA methylation between workers with non-active and active ovaries in any comparisons. Similarly, non-treated and CO2-treated workers are not significantly different in DNA methylation pattern. For the last part, DNA methylation at target region of Krüppel homolog-1 (Kr-h1) is determined in 7-day old queenless workers. Bisulfite sequencing shows 12 methylated CpG sites found across amplified region of Kr-h1 in examined workers. The overall DNA methylation level of Kr-h1 is significantly higher in non-active ovaries than active ovaries. CO2 narcosis does not have a significant effect to the DNA methylation change in Kr-h1 in worker ovaries. In addition, it is found that there is one deletion site specific to one site of CpG methylation.
DOI: 10.14457/cu.the.2015.354
title: Antioxidant and antiproliferative activities of peptide from SEA LETTUCE Ulva rigida

description: The purpose of this study was to preparation protein hydrolysates from sea lettuce by enzymatic hydrolysis of three microbial proteases (alcalase, flavourzyme, and neutrase) and investigates antioxidant activity by determination of the DPPH, ABTS, and NO scavenging activity. The result showed protein hydrolysate by five percent flavourzyme exhibited higher antioxidant activities than those other enzymes. These peptides were fractionated by ultrafiltration and gel filtration chromatography, low molecular weight (F5) of peptide was the highest antioxidant activity. Peptide fractions were observed for their antioxidant activities including enzymatic SOD, Catalase and GPx scavenging activity. Additionally, using cytotoxicity activity was determined by MTT assay in five cell lines. The results revealed that F5 had more inhibition against the proliferation of KATO-III cell lines and SW620 cell lines. The F5 could induce apoptosis cells in KATO-III cell lines of IC50=1.23 µg/ml and SW620 cell lines of IC50=1.00 µg/ml. These peptides were identified by HPLC technique and mass spectrometry as 4 peptide chains. All peptide chains contain hydrophobic amino acids (Pro, Leu, Ala, Trp and Phe) and some peptide chain had aromatic amino acids (Phe, Trp and Tyr) to possess high antioxidant activity. However, sea lettuce stills a good source of natural antioxidant and might be used in future.
DOI: 10.14457/cu.the.2015.355
title: DEVELOPMENT OF PORTABLE SPECTROPHOTOMETRIC DEVICE WITH FLOW-BASED ANALYSIS SYSTEM FOR DETECTION OF HEAVY METAL IONS

description: Lead (II) and cadmium (II) were determined by a flow-based analysis with spectrophotometric detection. Quercetin (Querc) and cetyltrimethylammonium bromide (CTAB) were used as a complexing agent and a surfactant, respectively. The absorbance of the yellow-colored complexes (Pb-Querc and Cd-Querc) were measured in Tris(hydroxymethyl)aminomethane buffer (pH 8.9) within the wavelength of 420-470 nm. The optimal condition was obtained by using 800 mgL-1 quercetin reagent (20 µL) in solution with 3.0 mmol L-1 CTAB (150 µL). The sample solutions were determined by a sequential injection spectrophotometer using deionized water as a carrier. The linear relationship between absorbance and concentration of metals were obtained over the concentration range from 20-100 µg L-1 for both Cd (II) and Pb (II). The limit of detection (LOD) of Cd (II) was 2 µg L-1 and the limit of quantification (LOQ) was 8 µg L-1. The limit of detection (LOD) of Pb (II) was 2 µg L-1 and the limit of quantification (LOQ) was 7 µg L-1. The relative standard deviations were 3.6% (n=7) and 3.4% (n=7) for 20 µg L-1 Cd (II) and Pb (II), respectively. The optimal condition was applied with the developed device for the determination of Cd (II) and Pb (II) in water. The alternative light source, LED (light-emitting diode), was also used for a miniaturization purpose.
DOI: 10.14457/cu.the.2015.356
title: PNA IMMOBILIZED ON PAPER FOR DNA DETECTION

description: In the past few years, DNA sensors based on immobilized DNA oligonucleotides have become an essential tool in DNA analysis. However, sensitivity and selectivity were still problematic in many applications. One efficient way to improve the performance of DNA sensors was a replacement of traditional DNA probe by other DNA mimics which possessed superior properties. AcpcPNA is an attractive candidate DNA replacement because of its excellent stability and specificity of the acpcPNA-DNA hybrid. In this work, the acpcPNA was immobilized on cellulose paper by employing divinyl sulfone as a crosslinker. AcpcPNA acting as a capture probe rapidly hybridized with DNA target which was delivered through capillary transport. After that, DNA detection was carried out by simple staining with azure A. The results showed high specificity which can clearly discriminate single-base mismatch although the sensitivity was somewhat low (detection limit was 3.3 pmol). The signal intensity could have been theoretically enhanced by increasing the length of hanging sequence; nevertheless, preliminary experiments suggest that longer sequence led to more steric effect and therefore the detection of very long DNA sequences still requires further development. In conclusion, a prototype of paper-based macroarray with immobilized acpcPNA probes for DNA detection was developed for a simple and rapid label-free DNA detection with high specificity.
DOI: 10.14457/cu.the.2015.357
title: Taxonomic revision of fern genus Tectaria Cav. (Tectariaceae) in Thailand

description: Due to a great number of variations in morphological characters and the ambiguity of some diagnostic characters using in a key and descriptions of Tectaria Cav. in the Flora of Thailand leading to confusing in species determination in most Thai herbaria. Moreover, some new taxa were added to the Thai Flora without any amended key-to-species. On a worldwide basis the genus boundary of Tectaria was extended to include some related genera based on recent phylogenetic studies. Thus, the preceding taxonomic information of Thai Tectaria was inappropriate. The objective of this research was to revise the genus Tectaria in Thailand based on the existing herbarium specimens in Thailand and abroad, together with some additional field collections. Intensive morphological, anatomical and palynological examinations were carried out, especially in pair/group of confused species. Frond and scale characters, venation patterns, sori distribution, and perispore ornamentation are important and useful characters for species delimitation. Fifty-four species are recognized which included five species of the previously recognized Heterogonium C. Presl and Quercifilix Copel. Among these 11 species are new records, viz. T. acrocarpa (Ching) Christenh., T. aurita (Sw.) S. Chandra, T. chattagramica (C.B. Clarke) Ching, T. hymenophylla (Parish ex Bedd.) Holttum, T. kehdingiana (Kuhn) M.G. Price, T. multicaudata (C.B. Clarke) Ching, T. poilanei Tardieu, T. subpedata (Harr.) Ching, T. subvariolosa S.Y. Dong, T. trichotoma (Fée) Tagawa and T. zippelii S.Y. Dong, nine species tend to be new to science and 11 species are endemic to Thailand. Lectotypification of 16 species and epitypification of T. keckii are proposed. A key to the species is provided, as well as descriptions, illustrations, photograph, synonymy, and a list of examined specimens for each species. The evaluation of some critically endangered species are discussed.
DOI: 10.14457/cu.the.2015.358
title: Construction of designs from symplectic graphs and orthogonnal graphs over finite local rings

description: In this thesis, we construct designs from symplectic graphs and orthgonal graphs over finite local rings. We use duplications of points and blocks and param- eters of subconstiuents of graphs. Moreover, we obtain the parameters of directed graphs from these designs.
DOI: 10.14457/cu.the.2015.359
title: Algebraic properties of gyrogroups

description: In the first part of the dissertation, we give an algebraic proof that the open unit ball B of Euclidean space Rn, equipped with Einstein addition E, forms a uniquely 2-divisible gyrocommutative gyrogroup or, equivalently, a B-loop, using the Clifford algebra formalism. As a consequence, we obtain a compact formula for Einstein addition in terms of Möbius addition. We then give a characterization of associativity and commutativity of vectors in  with respect to Einstein addition. In the second part of the dissertation, we study gyrogroups from an algebraic point of view. We extend some well-known theorems in group theory to gyrogroups, including Cayley’s theorem, the isomorphism theorems, and Lagrange’s theorem. We also prove that gyro-groups of particular order satisfy the Cauchy property.
DOI: 10.14457/cu.the.2015.360
title: Personal identification by recognition of EEG power spectrogram having short processing time

description: Electroencephalography (EEG) is the electrical measurement of brain activity. EEG is an interesting signal in biometrics on account of individuality and arduous to imitate the signal patterns. This research concentrates on person identification using power spectrogram without composite EEG cleaning. The underlying technique encompasses feature extraction by using spectrogram to determine dominant values from spectrogram with Singular Decomposition Value (SVD). The proposed method can separate a person from an outsider group yielding the highest accuracy at 100 percent classification in less than 1 second. The outcomes also reveal that the frontal area is an important location of scalp electrode placement in person identification.
DOI: 10.14457/cu.the.2015.362
title: Alternative quadratic functional equation on 2-divisible abelian groups

description: Let (G+) be a 2-divisible abelian group and let (Y+) be a real (or complex or rational) linear space. We will show that the alternative quadratic function equation ʄ(x+y) + ʄ(x-y) = 2ʄ(x)±2ʄ(y) is equivalent to the classical quadratic functional equation ʄ(x+y) + ʄ(x-y) = 2ʄ(x)+2ʄ(y) for the class of functions ʄ : G→Y
DOI: 10.14457/cu.the.2015.363
title: Alternative jensen functional equation on groups

description: Given integers α, β, γ such that (α, β, γ) ̸= k(1,−2, 1) for all k ∈ Z, we will establish a criterion for the existence of the general solution of the alternative Jensen functional equation of the form f(xy^{−1}) − 2f(x) + f(xy) = 0 or αf(xy^{−1}) + βf(x) + γf(xy) = 0, where f is a mapping from a group (G, ·) to a uniquely divisible abelian group (H, +).
DOI: 10.14457/cu.the.2015.364
title: Digraphs of the kTH power mapping over some finite commutative rings

description: In this dissertation, we consider a local extension of the Galois ring of the form GR(pᵑ,d)[x]/(ʄ(x)ͣ), where n d and a are positive integers p is a prime and ʄ(x) is a monic polynomial in GR(pᵑ,d)[x]/ of degree r such that the reduction in is irreducible. We establish the exponent of R without completely determination of its unit group structure. We obtain better analysis of the iteration graphs G(k) ® induced from the k-th power mapping including the conditions on symmetric digraphs. In addition, we work on the digraph over a finite chain ring R. The structure of G(k)2 such as indeg (k) 0 and maximum distance for G(k)2 ® is determined by the nilpotency of maximal ideal M of R.
DOI: 10.14457/cu.the.2015.365
title: Edge-odd graceful labelings of prism-like graphs of cycles

description: A simple graph G with q edges is called an edge-odd graceful graph, if there is a bijection f from the edge set of the graph to {1,3,5,…,2q-1} such that, when each vertex is assigned the sum of all values of the edges incident to it modulo 2q, the resulting vertex labels are distinct. In this thesis, we define new prism-like graphs and prove that they are edge-odd graceful graphs.
DOI: 10.14457/cu.the.2015.366
title: Generalizations of Schweizer-Wolff measure of dependence
DOI: 10.14457/cu.the.2015.367
title: POUR POINT DEPRESSANTS FROM DICARBOXYLIC ACID ALKYL ESTERS FOR PALM BIODIESEL

description: This research aims to synthesize pour point depressants from three types of dicarboxylic acids including 1,4-cyclohexanedicarboxylic acid, isophthalic acid and terephthalic acid by esterification using five types of alcohols including 1-propanol, 2-propanol, 1-butanol, 2-butanol and 2-methyl-1-propanol to improve flow properties at low temperature of palm biodiesel. These synthetic pour point depressants were characterized by proton nuclear magnetic resonance spectroscopy technique. The flow properties at low temperature of palm biodiesel were tested according to ASTM D 2500 and ASTM D 97 by adding pour point depressants at 10,000, 50,000, 100,000, 150,000 and 200,000 ppm. The result indicated that di 2-butyl-terephthalate exhibited the best improvement of flow properties at low temperature of palm biodiesel. It can reduce cloud point value from 16.5 °C to 12.0 °C and pour point value from 11.5 °C to 3.0 °C at 200,000 ppm.
DOI: 10.14457/cu.the.2015.368
title: APPLYING DIGITAL SIGNATURE TO PRINTOUT FOR TRUSTWORTHY VERIFICATION USING IMAGE HASH

description: Most certificate or document issuing processes are in a form of printing document to a paper. Some might improve the trustworthiness of their paper via using secure paper or secure printing method such as watermark, hologram etc. The interesting problem is that this security mechanism is not enough because nowadays printer with secure printing module can be obtained easier than before, which means reproducing counterfeit document can be done uncomplicatedly. To solve this problem, a security framework using image hashing function to capture document fingerprint and perform a digital signature based on PKI infrastructures is proposed to guarantee this fingerprint integrity. This framework consists of three important components. The first component is image hashing function for capturing perceptual appearance of printing document and it is then used to verify integrity of their document. The second component is digital signature based on PKI infrastructure for protecting image hash value from modification, authenticating document signer and guaranteeing content commitment from the document signer. The last important component of this proposed framework is 2D barcode for embedding result from the digital signature process that belongs to their printing document.
DOI: 10.14457/cu.the.2015.369
title: Invariance properties of dependence measures

description: Li gave a generalization of non-symmetric copula-based dependence measure, such as the Trutschnig\'s measure of dependence. A precise sufficient condition which makes Li\'s generalization a non-symmetric measure of dependence is given and proved rigorously. Supported by its non-symmetric dependence measure properties, we symmetrize the Li\'s non-symmetric measure of dependence and investigate its properties. Specifically, we analyze the key properties of dependence measures including well-defined property, abilities to detect independence and dependence at the two extreme values $0,1$ respectively, and invariance under the certain types of transformations. In particular, we find, via several examples, that a dependence measure possessing an ability to detect a larger class of dependences tends to be invariant under an accordingly large class of transformations. The probabilistic version of maximal information coefficient (MIC) is also proved to be a dependence measure. Lastly, we show that there does not exist a dependence measure which is both invariant under strictly monotonic transformations and able to catch complete dependence.
DOI: 10.14457/cu.the.2015.370
title: High accuracy prediction of human papillomavirus types by statistical chaos representation and reduced dimensional quantization

description: HPV genotyping is a significant approach to provide better diagnosis, medical treatment, and prevention strategies for fighting with cervical cancers. Firstly, ChaosCentroid and ChaosFrequency feature extraction techniques were proposed for HPV genotype prediction from whole genomes. ChaosCentroid captures the structure of nucleotide subsequences in terms of centroid, while ChaosFrequency extracts the statistical distribution of the subsequences along genomes. For predicting systems, multi-layer perceptron, radial basis function, k-nearest neighbor, and fuzzy k-nearest neighbor techniques were deployed. The experimental results showed that all methods yielded the highest prediction performance among the results obtained from several compared methods. But time complexity of the proposed techniques was considerably lower than the comparative alignment method. Secondly, ChaosPoly feature extraction technique was subsequently proposed for HPV genotype prediction from partial coding sequences. For each sub-region, ChaosPoly gives the precedence to the distribution of dot patterns in the chaos game representation in a form of polynomial. The fuzzy k nearest neighbor technique was deployed for identifying the corresponding HPV genotypes. The results showed that ChaosPoly outperforms ChaosCentroid and ChaosFrequency.
DOI: 10.14457/cu.the.2015.371
title: Mutagenesis of amylomaltase from corynebacterium glutamicum ATCC 13032 to improve thermostability of the enzyme

description: งานวิจัยนี้มีวัตถุประสงค์เพื่อปรับปรุงการทนความร้อนของเอนไซม์แอมิโลมอลเทสจาก Corynebacterium glutamicum ด้วยวิธีการกลายยีนแบบสุ่ม และการกลายยีนแบบเฉพาะตำแหน่ง จากผลการกลายยีนแบบสุ่มด้วยเทคนิค error-prone PCR พบว่าสามารถคัดเลือกเอนไซม์แอมิโลมอลเทสกลายที่ทนอุณหภูมิ 50 ºซ ได้ 1 โคลนในขณะที่เอนไซม์ดั้งเดิมสูญเสียแอกทิวิตีเกือบทั้งหมด ตรวจสอบพบว่าเอนไซม์กลายมีกรดอะมิโนกลายจากเอนไซม์ดั้งเดิม 1 ตำแหน่งคือ A406V เพื่อตรวจสอบว่าตำแหน่ง Ala406 มีผลต่อการทนความร้อนของเอนไซม์ จึงใช้เทคนิคการกลายยีนแบบเฉพาะตำแหน่งในการกลายยีนให้เป็น A406V และ A406L เมื่อนำเอนไซม์กลายทั้ง 2 ชนิดไปตรวจสอบลักษณะสมบัติพบว่า เอนไซม์กลาย A406V และA406L เร่งปฏิกิริยา transglucosylation ได้สูงขึ้นจากเอนไซม์ดั้งเดิม มีอุณหภูมิ และ pH ที่เหมาะสม สำหรับปฏิกิริยา disproportionation และ cyclization เพิ่มขึ้นจากเอนไซม์ดั้งเดิม และ สามารถทนอุณหภูมิที่ 35 ºซ และ 40 ºซ ได้ดีกว่า นอกจากนี้ผลของการทำ DSC พบว่าเอนไซม์กลายทั้ง 2 ชนิดมีรูปแบบการคงทนต่อความร้อนที่อุณหภูมิสูงขึ้นกว่าเอนไซม์ดั้งเดิม โดยที่เอนไซม์ A406V แสดงผลของการเร่งปฎิกิริยา และทนต่อความร้อนได้ดีกว่า A406L จากการศึกษาทางจลนพลศาสตร์ของปฎิกิริยา disproportionation โดยใช้มอลโทไทรโอสเป็นซับสเทรต พบว่า เอนไซม์ A406V และ A406L มีค่า kcat/Km สูงขึ้นกว่าเอนไซม์ดั้งเดิม 2.9 และ 1.4 เท่า ตามลำดับ โดยมีการเพิ่มอย่างมีนัยสำคัญของ kcat เมื่อตรวจสอบผลิตภัณฑ์ LR-CDs ของปฎิกิริยา cyclization พบว่าเอนไซม์ A406V สามารถผลิต LR-CDs ที่มีขนาดใหญ่และจำนวนที่มากขึ้นเมื่อเมื่อเทียบกับเอนไซม์ดั้งเดิม โดยเฉพาะเมื่อเพิ่มระยะเวลา และอุณหภูมิในการบ่มให้สูงขึ้น ในส่วนที่สองของงาน ได้สร้างเอนไซม์กลายเพิ่มขึ้น เพื่อปรับปรุงการทนร้อนให้ดีขึ้น โดยใช้เทคนิคการกลายยีนเฉพาะตำแหน่ง เปลี่ยนกรดอะมิโน Ala406 ให้เป็น His (H), Arg (R) และ Phe (F) และเปลี่ยนที่ตำแหน่ง Asn287 ให้เป็น Tyr (Y) เมื่อตรวจสอบลักษณะสมบัติ พบว่า เอนไซม์กลายทั้ง 4 ชนิด A406H, A406R, A406F และ N287Y เร่งปฏิกิริยา transglucosylation ลดลงอย่างเห็นได้ชัด ในขณะที่ไม่สามารถตรวจวัดแอกทิวิตีของ cyclizationได้ เอนไซม์กลายทั้ง 4 ชนิด มี optimum pH สำหรับปฏิกิริยา disproportionation เพิ่มขึ้น โดยที่ เอนไซม์กลาย N287Y เพิ่มขึ้น 0.5 pH unit ขณะที่ เอนไซม์กลาย A406H, A406R, A406F เพิ่มขึ้น 1.0 pH unit ผลที่สำคัญคือ เอนไซม์กลายทั้ง 4 ชนิด สามารถทนร้อนที่อุณหภูมิ 35 oซ, 40 oซ และ 45 oซ ได้สูงกว่าเอนไซม์ดั้งเดิม เมื่อบ่มในระยะเวลา 3 ชม. และยังพบว่า เอนไซม์กลาย N287Y มี optimum temperature สำหรับปฏิกิริยา disproportionation เพิ่มขึ้น โดยเมื่อทำการตรวจสอบโครงรูปด้วยวิธี circular dichroism และรูปแบบการคงทนต่อความร้อนด้วยเทคนิค DSC พบว่า โครงสร้างทุติยภูมิของเอนไซม์กลายแทบไม่เปลี่ยนแปลง แต่โครงสร้างตติยภูมิเปลี่ยนไปเมื่อเทียบกับเอนไซม์ดั้งเดิม จากผลทั้งหมด พบว่า เอนไซม์กลายที่ทนร้อนดีที่สุด และมีแอกทิวิตีสูงกว่าเอนไซม์ดั้งเดิมคือ A406V
DOI: 10.14457/cu.the.2015.372
title: Cloning and expression of single chain variable fragment of monoclonal antibody against norfloxacin in Pichia pastoris

description: Currently, recombinant antibody technology has provided an alternative approach for engineering low-cost antibodies with desirable affinities and specificities. In this research, a single chain variable fragment (scFv) that recognizes norfloxacin antibiotic was successfully designed and constructed. The cDNA was synthesized from total RNA of antinorfloxacin-producing hybridoma clone (Nor155). DNA encoding VH and VL regions of this hybridoma were amplified and sequenced. DNA fragments of VH and VL regions were approximately 402 bp and 363 bp, respectively. The scFv of Nor155 was constructed by addition of (Gly4Ser)3 as a linker between VH and VL regions and subcloned into pPICZαA, an expression vector of Pichia pastoris, to generate pPICZαA-scFv (pJM01). After sequencing analysis, the scFv antibody gene cassette revealed an open reading frame of 1161 bp with the potential to encode a polypeptide of 386 amino acids and a predicted molecular weight of 42.5 kDa (intracellular) and 32.67 kDa (secreted), respectively. The pJM01 was incorporated into the Pichia chromosome at AOX1 promoter and transformants were confirmed by Southern blot analysis, PCR and sequencing analysis. Southern blot analysis was carried out in 9 transformants, which showed that the scFv gene was integrated into the Pichia chromosome. By performing the scFv expression in shaker culture, transformant O5 showed the highest scFv expression, among 9 transformants. The mRNA transcript level of transformant O5 showed highest relative normalized expression at 24 h, 688 times compared to the control. The recombinant scFv antibody was purified by immobilized metal affinity chromatography (IMAC). SDS-PAGE and Western blot revealed a predominant protein at 32.67 kDa of the purified scFv, thus indicating the scFv antibody was successfully expressed by P. pastoris. The specific binding and affinity in ELISA and surface plasmon resonance (SPR) indicated that the purified scFv still functional in term of antigen binding. From all results, it could be concluded that scFv of monoclonal antibody Nor155 could be produced in yeast. In addition, the binding ability of the obtained scFv to the corresponding antigen remains functional.