cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1246728.v2
title: Evaluation of the DNA barcoding approach to develop a reference data-set for the threatened flora of Sicily

description: The Mediterranean Basin is one of the most significantly altered World Biodiversity Hotspots with extensive habitat loss and fast genetic population erosion, for which urgent biodiversity reconnaissance and preservation actions are required. In particular, Sicily has about 600 taxa classified as threatened or near-threatened. The correct recognition and identification of such biodiversity is required for supporting further activities. The objective of this work is to assess the ability of the DNA barcoding approach to identify different taxonomic groups from a collection of the most threatened plant taxa, throughout natural Sicilian populations. The evaluation of the DNA barcoding core markers, <i>rbc</i>L and <i>mat</i>K, was carried out on 30 taxa belonging to 13 families. DNA barcode fragments were recovered from all taxa (100%). The <i>rbc</i>L gene was recovered from 97% of the taxa and <i>mat</i>K gene from 73%. In this test, 19 taxa overall (63%) were totally resolved at the specific or subspecific level, by at least one of the core markers. Fourteen of the 17 most threatened taxa (EN, CR) included in this work were totally discriminated. The <i>mat</i>K and <i>rbc</i>L locus, respectively, resolved 64% and 48% of the taxa successfully sequenced. The <i>mat</i>K gene expressed the highest genetic distance (K2P value), from 0.4% to 8.6%, against a range of 0.1–2% of <i>rbc</i>L gene. However, the <i>rbc</i>L gene appeared a good compromise between PCR, sequencing success and species-level resolution. Cryptic groups suggest the implementation of additional barcoding markers or different primer combinations, particularly for <i>mat</i>K, in order to increase the performances. However, this preliminary result confirms the potential of the barcoding approach for quick identification of unknown and heterogeneous plant groups to generate a dedicated reference data-set of the threatened Sicilian flora for a wide range of applications.
DOI: 10.6084/m9.figshare.1246696
title: 48 h incubation of HeLa cells with 5% CALNNTAT and 95% PEG (mole/mole) capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 48 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Four different cell sections were imaged (35 images) to probe the localisation of gold nanoparticles.
DOI: 10.6084/m9.figshare.1246696.v1
title: 48 h incubation of HeLa cells with 5% CALNNTAT and 95% PEG (mole/mole) capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 48 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Four different cell sections were imaged (35 images) to probe the localisation of gold nanoparticles.
DOI: 10.6084/m9.figshare.1246722.v1
title: Multiannual phytoplankton trends in relation to environmental changes across aquatic domains: a case study from Sardinia (Mediterranean Sea)

description: We investigated multiannual trends in phytoplankton in relation to several environmental drivers. We analyzed ecological data collected during the last two decades from three aquatic sites: an artificial lake, a coastal lagoon, and a marine coastal area. Hydrographic-, nutrient-, and phytoplankton- data were statistically analyzed to detect trends and interactions. In all ecosystems, the chlorophyll <i>a</i> concentration decreased with increasing abundance of small-sized phytoplankton. Phytoplankton dynamics were related to decreased nutrient concentrations in the lake, to dynamics of phosphorus and decreased salinity in the lagoon, and probably to combined top-down and bottom-up processes in the marine gulf.
DOI: 10.6084/m9.figshare.1246726.v1
title: The fate of surface cell layers of <i>Daucus carota</i> (L.) embryos raised in suspension culture

description: The ultrastructure and fate of surface cells covering mature somatic embryos of <i>Daucus carota</i> grown in suspension culture were analyzed and new information obtained concerning somatic embryogenesis in these conditions. Our studies showed that, during some developmental stages, these embryos were covered irregularly and discontinuously by cells with a typical protodermal phenotype characterized by a cuticle on the outer cell wall. We observed that cells with cuticle were peeled off from the surface of mature embryos. Before peeling off, these cells underwent programmed cell death (PCD), which was confirmed by the TUNEL method. Transmission electron microscopy revealed advanced processes of autophagy in these cells.
DOI: 10.6084/m9.figshare.1246728.v1
title: Evaluation of the DNA barcoding approach to develop a reference dataset for the threatened flora of Sicily

description: The Mediterranean basin is one of the most significantly altered World Biodiversity Hotspots with extensive habitat loss and fast genetic population erosion, for which urgent biodiversity reconnaissance and preservation actions are required. In particular, Sicily has about 600 taxa classified as threatened or near-threatened. The correct recognition and identification of such biodiversity is required for supporting further activities.
DOI: 10.6084/m9.figshare.1246660
title: 24 h incubation of HeLa cells with 5% CALNNTAT and 95% PEG capped (mole/mole) capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 24 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Five different cell sections were imaged (36 images) to probe the localisation of gold nanoparticle.
DOI: 10.6084/m9.figshare.1246722
title: Multiannual phytoplankton trends in relation to environmental changes across aquatic domains: A case study from Sardinia (Mediterranean Sea)

description: We investigated multiannual trends in phytoplankton in relation to several environmental drivers. We analyzed ecological data collected during the past two decades from three aquatic sites: an artificial lake, a coastal lagoon, and a marine coastal area. Hydrographic, nutrient, and phytoplankton data were statistically analyzed to detect trends and interactions. In all ecosystems, the chlorophyll <i>a</i> concentration decreased with increasing abundance of small-sized phytoplankton. Phytoplankton dynamics were related to decreased nutrient concentrations in the lake, to dynamics of phosphorus and decreased salinity in the lagoon, and probably to combined top-down and bottom-up processes in the marine gulf.
DOI: 10.6084/m9.figshare.1246622
title: 2 h incubation of HeLa cell with 100% PEG capped gold nanoparticles

description: HeLa cells were incubated with 100% PEG capped gold nanoparticles for 2 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Five cell sections were imaged (12 images).
DOI: 10.6084/m9.figshare.1246722.v3
title: Multiannual phytoplankton trends in relation to environmental changes across aquatic domains: A case study from Sardinia (Mediterranean Sea)

description: We investigated multiannual trends in phytoplankton in relation to several environmental drivers. We analyzed ecological data collected during the past two decades from three aquatic sites: an artificial lake, a coastal lagoon, and a marine coastal area. Hydrographic, nutrient, and phytoplankton data were statistically analyzed to detect trends and interactions. In all ecosystems, the chlorophyll <i>a</i> concentration decreased with increasing abundance of small-sized phytoplankton. Phytoplankton dynamics were related to decreased nutrient concentrations in the lake, to dynamics of phosphorus and decreased salinity in the lagoon, and probably to combined top-down and bottom-up processes in the marine gulf.
DOI: 10.6084/m9.figshare.1246622.v1
title: 2 h incubation of HeLa cell with 100% PEG capped gold nanoparticles

description: HeLa cells were incubated with 100% PEG capped gold nanoparticles for 2 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Five cell sections were imaged (12 images).
DOI: 10.6084/m9.figshare.1246660.v1
title: 24 h incubation of HeLa cells with 5% CALNNTAT and 95% PEG capped (mole/mole) capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 24 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Five different cell sections were imaged (36 images) to probe the localisation of gold nanoparticle.
DOI: 10.6084/m9.figshare.1246458
title: 10 min incubation with 5% CALNNTAT and 95% PEG capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 10 min. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Five cell sections were imaged (41 images). The experimental details can be found in the article.
DOI: 10.6084/m9.figshare.1246609
title: 2 h incubation of HeLa cells with 5% CALNNTAT and 95% PEG capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 2 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Six cell sections were imaged (138 images). The experimental details can be found in the article.
DOI: 10.6084/m9.figshare.1246725.v1
title: Clinical Research: A Globalized Network

description: These files relate to data extracted from ClinicalTrials.gov. In the database file, data for individual clinical trials are included, and attributes include study identifier, study type, trial dates, interventions, sample size, countries in which the study was conducted, etc. The Edges file contains geographic data derived from the clinical trials data set that can be used to generate networks to illustrate geographic connectivity through clinical research, using open access software such as Gephi.
DOI: 10.6084/m9.figshare.1246609.v1
title: 2 h incubation of HeLa cells with 5% CALNNTAT and 95% PEG capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 2 h. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Six cell sections were imaged (138 images). The experimental details can be found in the article.
DOI: 10.6084/m9.figshare.1246722.v2
title: Multiannual phytoplankton trends in relation to environmental changes across aquatic domains: A case study from Sardinia (Mediterranean Sea)

description: We investigated multiannual trends in phytoplankton in relation to several environmental drivers. We analyzed ecological data collected during the past two decades from three aquatic sites: an artificial lake, a coastal lagoon, and a marine coastal area. Hydrographic, nutrient, and phytoplankton data were statistically analyzed to detect trends and interactions. In all ecosystems, the chlorophyll <i>a</i> concentration decreased with increasing abundance of small-sized phytoplankton. Phytoplankton dynamics were related to decreased nutrient concentrations in the lake, to dynamics of phosphorus and decreased salinity in the lagoon, and probably to combined top-down and bottom-up processes in the marine gulf.
DOI: 10.6084/m9.figshare.1246458.v1
title: 10 min incubation with 5% CALNNTAT and 95% PEG capped gold nanoparticles

description: HeLa cells were incubated with 5% CALNNTAT, 95% PEG (mole/mole) capped gold nanoparticles for 10 min. The gold nanoparticles containing medium was then removed and cells were washed three times with warm 1 x PBS. The cells were then fixed and processed for TEM. Five cell sections were imaged (41 images). The experimental details can be found in the article.
DOI: 10.6084/m9.figshare.1246623.v1
title: Pollen morphology and localization of Ni in some Ni-hyperaccumulator taxa of <i>Alyssum</i> L. (Brassicaceae)

description: Pollen morphology of seven <i>Alyssum</i> L. taxa growing on serpentine soils in different places in the European Mediterranean macrobioclimate territory were studied, described and compared. Cluster analysis was performed to show similarity between species and their populations. The shape of the pollen grains varies among the species and among the grains within the same anther. The pollen grains are 3-colpate, prolate, with long and narrow colpi reaching the poles. The ornamentation of the exine varies from micro-reticulate to reticulate between the species. Pollen sterility/fertility was also calculated. The highest percentage of sterile pollen (73.76%) was calculated for <i>Alyssum murale</i> subsp. <i>murale</i> and the lowest (9.54%) for <i>A. bertolonii</i> subsp<i>. bertolonii</i>. All species are representatives of sect. Odontarrhena (C.A.Meyer) Koch well known as Ni-hyperaccumulators. Nickel and other elements present in pollen and stamen were studied by inductively coupled plasma-mass spectrometry (ICP-MS). The stamen parts of all species were micromorphologically analyzed by scanning electron microscopy (SEM) coupled to an Energy-Dispersive X-Ray Probe (EDX). Accumulation of Ni was detected in the stamens of all studied species and rarely in the pollen grains. The distribution patterns of Ni were similar among species examined.
DOI: 10.6084/m9.figshare.1246261
title: Gaussian Job Archive for F2I6

description: Archive of work produced using the resources of the Imperial College High Performance Computing Service.
DOI: 10.6084/m9.figshare.1246623
title: Pollen morphology and localization of Ni in some Ni-hyperaccumulator taxa of <i>Alyssum</i> L. (Brassicaceae)

description: Pollen morphology of seven <i>Alyssum</i> L. taxa growing on serpentine soils in different places in the European Mediterranean macrobioclimate territory were studied, described and compared. Cluster analysis was performed to show similarity between species and their populations. The shape of the pollen grains varies among the species and among the grains within the same anther. The pollen grains are 3-colpate, prolate, with long and narrow colpi reaching the poles. The ornamentation of the exine varies from micro-reticulate to reticulate between the species. Pollen sterility/fertility was also calculated. The highest percentage of sterile pollen (73.76%) was calculated for <i>Alyssum murale</i> subsp. <i>murale</i> and the lowest (9.54%) for <i>A. bertolonii</i> subsp<i>. bertolonii</i>. All species are representatives of sect. Odontarrhena (C.A. Meyer) Koch well known as Ni-hyperaccumulators. Nickel and other elements present in pollen and stamen were studied by inductively coupled plasma-mass spectrometry. The stamen parts of all species were micromorphologically analyzed by scanning electron microscopy coupled to an energy-dispersive X-ray probe. Accumulation of Ni was detected in the stamens of all studied species and rarely in the pollen grains. The distribution patterns of Ni were similar among the species examined.
DOI: 10.6084/m9.figshare.1246623.v3
title: Pollen morphology and localization of Ni in some Ni-hyperaccumulator taxa of <i>Alyssum</i> L. (Brassicaceae)

description: Pollen morphology of seven <i>Alyssum</i> L. taxa growing on serpentine soils in different places in the European Mediterranean macrobioclimate territory were studied, described and compared. Cluster analysis was performed to show similarity between species and their populations. The shape of the pollen grains varies among the species and among the grains within the same anther. The pollen grains are 3-colpate, prolate, with long and narrow colpi reaching the poles. The ornamentation of the exine varies from micro-reticulate to reticulate between the species. Pollen sterility/fertility was also calculated. The highest percentage of sterile pollen (73.76%) was calculated for <i>Alyssum murale</i> subsp. <i>murale</i> and the lowest (9.54%) for <i>A. bertolonii</i> subsp<i>. bertolonii</i>. All species are representatives of sect. Odontarrhena (C.A. Meyer) Koch well known as Ni-hyperaccumulators. Nickel and other elements present in pollen and stamen were studied by inductively coupled plasma-mass spectrometry. The stamen parts of all species were micromorphologically analyzed by scanning electron microscopy coupled to an energy-dispersive X-ray probe. Accumulation of Ni was detected in the stamens of all studied species and rarely in the pollen grains. The distribution patterns of Ni were similar among the species examined.
DOI: 10.6084/m9.figshare.1246725.v2
title: Clinical Research: A Globalized Network

description: These files relate to data extracted from ClinicalTrials.gov. In the database file, data for individual clinical trials are included, and attributes include study identifier, study type, trial dates, interventions, sample size, countries in which the study was conducted, etc. The Edges file contains geographic data derived from the clinical trials data set that can be used to generate networks to illustrate geographic connectivity through clinical research, using open access software such as Gephi. The figures are network diagrams showing geographic connectivity among individual countries through common participation in multinational clinical trials. The thickness of the connecting lines (edges) reflects the strength of a connection.
DOI: 10.6084/m9.figshare.1246457
title: caja OIT

description: HOJA DE CONTROL DE LA CAJA DE HERRAMIENTAS DE CONTROL QUIMICO PEROXIDO DE HIDROGENO
DOI: 10.6084/m9.figshare.1246261.v1
title: Gaussian Job Archive for F2I6

description: Archive of work produced using the resources of the Imperial College High Performance Computing Service.
DOI: 10.6084/m9.figshare.1246623.v2
title: Pollen morphology and localization of Ni in some Ni-hyperaccumulator taxa of <i>Alyssum</i> L. (Brassicaceae)

description: Pollen morphology of seven <i>Alyssum</i> L. taxa growing on serpentine soils in different places in the European Mediterranean macrobioclimate territory were studied, described and compared. Cluster analysis was performed to show similarity between species and their populations. The shape of the pollen grains varies among the species and among the grains within the same anther. The pollen grains are 3-colpate, prolate, with long and narrow colpi reaching the poles. The ornamentation of the exine varies from micro-reticulate to reticulate between the species. Pollen sterility/fertility was also calculated. The highest percentage of sterile pollen (73.76%) was calculated for <i>Alyssum murale</i> subsp. <i>murale</i> and the lowest (9.54%) for <i>A. bertolonii</i> subsp<i>. bertolonii</i>. All species are representatives of sect. Odontarrhena (C.A. Meyer) Koch well known as Ni-hyperaccumulators. Nickel and other elements present in pollen and stamen were studied by inductively coupled plasma-mass spectrometry. The stamen parts of all species were micromorphologically analyzed by scanning electron microscopy coupled to an energy-dispersive X-ray probe. Accumulation of Ni was detected in the stamens of all studied species and rarely in the pollen grains. The distribution patterns of Ni were similar among the species examined.
DOI: 10.6084/m9.figshare.1246216
title: Effects of the workplace health promotion activities soccer and Zumba on muscle pain, work ability and perceived physical exertion among female hospital employees

description: Workplace health promotion
DOI: 10.6084/m9.figshare.1246216.v1
title: Effects of the workplace health promotion activities soccer and Zumba on muscle pain, work ability and perceived physical exertion among female hospital employees

description: Workplace health promotion
DOI: 10.6084/m9.figshare.1246203
title: Interindividual variability in functional connectivity as long-term correlate of temporal discounting

description: Intertemporal choice task and functional connectivity
DOI: 10.6084/m9.figshare.1246203.v1
title: Interindividual variability in functional connectivity as long-term correlate of temporal discounting

description: Intertemporal choice task and functional connectivity
DOI: 10.6084/m9.figshare.1245683
title: LA TRAMA DEL AMIANTO

description: Conclusiones e inquietudes sobre el documental "La trama del amianto" del programa La noche temática
DOI: 10.6084/m9.figshare.1245683.v1
title: LA TRAMA DEL AMIANTO

description: Conclusiones e inquietudes sobre el documental "La trama del amianto" del programa La noche temática
DOI: 10.6084/m9.figshare.1245571
title: Meta data lab report- Effect of density on abundance of crawling and flying insects

description: A transect will place in the grasslands near stong pond, and pan traps and pitfalls will be placed within a distnace of the trasect. Traps will be placed in thre diferrent<br>distinct habitats; low amount of plants, medium, and high. The quadrant can be manipulated by pulling out plants to make it one of the three categories. Plant abudance and plant density will be recorded. Plant density will be measured on a scale of low, medium,a nd high.<br>The two traps will be placed near each other (within 10 cm) and then checked to group the insects as either flying or non-flying based on their primary means of travel.<br>The experiment will be done twice in the span of 2 weeks. The traps are set around 2:30, then checked the next day after minimum of8-10 hours have passed.<br>A total of 30 trials will be conducted, 10 trials in each habitat, of low, medium, and high.
DOI: 10.6084/m9.figshare.1245649
title: Additional files: Study of antecendents and an intervension impact of proactive behavior

description: Here you can find additional files to article Is newcomer proactive behavior matter? Study of antecendents and an intervension impact
DOI: 10.6084/m9.figshare.1245109
title: OGrady Material Deposited in the Ambrose Monell Cryo Collection

description: I deposited a number of specimens at the AMCC based on the Hawaiian collections I made as a postdoctoral fellow in Rob DeSalle's lab. This fileset is composed of two files, one containing 54 accessions from 1998-2000 and another containing 180 accessions from 2002. These excel files contain more information than is available from the AMCC database search. Cross references to pinned vouchers present at AMNH, EMEC and BPBM will be added soon.
DOI: 10.6084/m9.figshare.1245106
title: Phylogenetic Tree of Enterobacter sp. str. UCD-UG_FMILLET

description: Phylogenetic tree created by using a full length 16S of <em>Enterobacter</em> sp. str. UCD-UG_FMILLET from our assembly and aligning it to 46 <em>Enterobacter</em> isolates and an Archaea outgroup from the Ribosomal Database Project (RDP). The phylogenetic tree was constructed using FastTree 2.
DOI: 10.6084/m9.figshare.1245105
title: Phylogenetic Tree of Burkholderia gladioli str. UCD-UG_CHAPALOTE

description: Phylogenetic tree created by using a full length 16S of <em>Burkholderia gladioli</em> str. UCD-UG_CHAPALOTE from our assembly and aligning it to 59 <em>Burkholderia</em> isolates and an Archaea outgroup from the Ribosomal Database Project (RDP). The phylogenetic tree was constructed using FastTree 2.