DOI: 10.5061/dryad.5hqbzkhcp
title: Data from: Ectomycorrhizal fungi are more sensitive to high soil nitrogen levels in forests exposed to nitrogen deposition

description: Ectomycorrhizal fungi are essential for nitrogen cycling in many temperate
 forests and responsive to anthropogenic nitrogen addition, which
 generally, decreases host carbon allocation to the fungi. In the boreal
 region, however, ectomycorrhizal fungal biomass has been found to
 correlate positively with soil nitrogen availability. Still, responses to
 anthropogenic input, for instance through atmospheric deposition, are
 commonly negative. To elucidate whether variation in nitrogen supply
 affects ectomycorrhizal fungi differently depending on geographical
 context, we investigated ectomycorrhizal fungal communities along two
 fertility gradients across nemo-boreal forests with similar ranges in soil
 N/C ratios and inorganic nitrogen availability but located in regions with
 contrasting rates of nitrogen deposition. Ectomycorrhizal biomass and
 community composition remained relatively stable across the
 nitrogen-gradient with low atmospheric nitrogen deposition, but biomass
 decreased, and the community changed more drastically, with increasing
 nitrogen availability in the gradient subjected to higher rates of
 nitrogen deposition. Moreover, potential activities of enzymes involved in
 ectomycorrhizal mobilisation of organic nitrogen decreased as N/C ratios
 increased. In forests with low external input, we propose that stabilising
 feedbacks in tree-fungal interactions maintain ectomycorrhizal fungal
 biomass and communities even in highly fertile soils. In contrast,
 anthropogenic nitrogen input seems to impair ectomycorrhizal functions.
description: Data belonging to: "Ectomycorrhizal fungi are more sensitive to high
 soil nitrogen levels in forests exposed to nitrogen deposition" by
 Jörgensen K., Clemmensen K., Wallander H., Lindahl BD. in New Phytologist
 *Important note about .csv files*\ all .csv files use "," and
 ";" delimiters which would limit re-use if not accounted for.
 The accompanying code on Zenodo does account for the formatting of the
 files. Description of column headings in the data sets: File:
 ECM_counts.csv Column name Description sample_ID unique sample sample ID
 tot_count total number of sequence counts Columns C:LG Sequence counts of
 fungal clusters obtained by single linkage clustering with bioinformatics
 pipeline SCATA. File: env_data.csv Column name Description site_ID Site ID
 Region Levels: 2 (S = nemoral, U = boreal) NH4_gOM continuous, mg
 ammonium-N per g organic matter NO3_gOM continuous, mg nitrate-N per g
 organic matter pH continuous, soil pH NAG_OM continuous,
 N-acetylglucosaminidase activity normalised per g organic matter aP_OM
 continuous, acid phosphatase activity normalised per g organic matter
 MnP_OM continuous, manganese peroxidase activity normalised per g organic
 matter N_C nitrogen/carbon ratio OM_cont organic matter content
 (proportional) File: ergosterol.csv Column name Description sample_ID
 Sample ID ergosterol_gOM continuous, normalised per g organic matter File:
 InorgN.csv Column name Description Site ID Site ID Month Levels: 3 (sep =
 September, okt = October, nov = November) NH4-N mg/gOM continuous, mg
 ammonium-N per g organic matter NO3-N mg/gOM continuous, mg nitrate-N per
 g organic matter File: N_dep_data.csv Column name Description site Site ID
 Year Numeric (years 1998-2018) reduced_N continuous, mg reduced N forms
 per m2 and year oxidized_N continuous, mg oxidized N forms per m2 and year
 File: EMF_clusters_UNITE_refs.xlsx Column name Description Cluster ID
 Cluster ID from the SCATA bioinformatics pipeline 2018 UNITE SH Reference
 to UNITE SH where applicable Columns C:K Taxonomy of ectomycorrhizal
 clusters. "n/a" is noted if taxonomy could not be assign to a
 certain level. Rep. seq. Ref- or Rep-seq in UNITE. "n/a" is used
 when there was no Ref- or Rep-seq in UNITE. Sequence1 Most common sequence
 in cluster from SCATA bioiinformatics pipeline