cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0sx6c32
title: CIL:41686, Homo sapiens, epidermoid carcinoma. In Cell Image Library

description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA022989 (shown in green). HHPA022989 is a polyclonal anti-CALCOCO2 antibody that labels vesicles. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
DOI: 10.6075/j0n878kp
title: CIL:41688, Homo sapiens, osteocarcinoma. In Cell Image Library

description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA026995 (shown in green). HPA026995is a polyclonal anti-POMP antibody that labels spots in the nuclei. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
DOI: 10.6075/j0qz28tf
title: CIL:41687, Homo sapiens, osteocarcinoma. In Cell Image Library

description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA023388 (shown in green). HPA023388 is a polyclonal anti-CARD14 antibody that labels aggresomes. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
DOI: 10.6075/j0qr4vxh
title: CIL:41678, Homo sapiens, epidermoid carcinoma. In Cell Image Library

description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA005963 (shown in green). HPA005963 is a polyclonal anti-CLIC3 antibody that labels spots in the nucleus. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
DOI: 10.6075/j0r2105k
title: CIL:41677, Homo sapiens, epidermoid carcinoma. In Cell Image Library

description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA002967 (shown in green). HPA002967 is a polyclonal anti-LRMP antibody that labels the nucleus, vesicles, plasma membrane, and cytoplasm. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
DOI: 10.6075/j0xw4hn1
title: CIL:41818, Arabidopsis thaliana. In Cell Image Library

description: Confocal micrograph of an Arabidopsis thaliana (thale cress) seedling. Honorable Mention, 2010 Olympus BioScapes Digital Imaging Competition©.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
DOI: 10.6075/j0jw8cs8
title: CIL:41815, Rattus. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: Midsaggital section of rat cerebellum, captured using confocal imaging. Section shows inositol trisphosphate receptor (IP3R) labelled in green, DNA in blue, and synaptophysin in magenta. Honorable Mention, 2010 Olympus BioScapes Digital Imaging Competition©.
DOI: 10.6075/j0br8r08
title: CIL:41816, common tree mushroom. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: modes of light microscopy; stereo microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in intrinsic optical density
Visualization methods: visualization of contiguous regions
description: The underside of a common tree mushroom captured using stereomicroscopy with 11 X magnification. Honorable Mention, 2010 Olympus BioScapes Digital Imaging Competition©.
DOI: 10.6075/j05x27s8
title: CIL:41738, Homo sapiens. In Cell Image Library

description: Colorized scanning electron micrograph of collagen/connective tissue removed from a human knee during arthroscopic surgery. The horizontal field width of the image is 16 microns. Wellcome Image Award 2012.
description: B0008288. 2011 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://images.wellcome.ac.uk/indexplus/page/Prices.html
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Processing history: colorized
DOI: 10.6075/j0ff3r7b
title: CIL:41737, Micrasterias denticulata. In Cell Image Library

description: Differential interference contrast micrograph of a Micrasterias, a type of green alga called a desmid. Each cell is constructed of two semicells which are mirror images of each other. The two semicells are joined by a narrow central bridge, or isthmus, containing the nucleus. Each semicell contains a single chloroplast. Micrasterias can reproduce asexually by binary fission (resulting in two separate cells, each of which has one of the parent's semicells and one new semicell). During this process, genetic material is duplicated and two new semicells grow between the original semicells. Micrasterias can also reproduce sexually through a process known as conjugation, which involves the transfer of genetic material between two cells. Wellcome Image Award 2012.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
description: 2011 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://images.wellcome.ac.uk/indexplus/page/Prices.html
DOI: 10.6075/j0nz86gs
title: CIL:41735, Homo sapiens. In Cell Image Library

description: This confocal and phase contrast image depicts the chemotactic behaviour of MDA-MB-231 breast cancer cells. The nuclei are labelled blue and the mitochondria are labelled red. The cells are squeezed into micro-scale channels to study large numbers of single cells migrating with varying concentrations of epidermal growth factor (EGF, shown in green) at the leading and trailing edge of the cell. The individual channels are 12 microns wide. Wellcome Image Award 2012.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of epitope; differences in amount of elastic light scattering
description: 0008497. 2011 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://images.wellcome.ac.uk/indexplus/page/Prices.html
DOI: 10.6075/j0ng4pg7
title: CIL:41674, Homo sapiens, osteocarcinoma. In Cell Image Library

description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA001842 (shown in green). HPA001842 is a polyclonal anti-PTK2 antibody that labels focal adhesions and the cytoplasm. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
DOI: 10.6075/j09w0dbn
title: CIL:41673, Homo sapiens, osteocarcinoma. In Cell Image Library

description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody 
 HPA001677 (shown in green). HPA001677 is a polyclonal anti-GOLIM4 antibody that labels the Golgi apparatus and the nucleus but not the nucleoli. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
DOI: 10.6075/j0h70dpf
title: CIL:41658, Gallus gallus, interneuron. In Cell Image Library

description: Confocal micrograph of ‚Äúlost‚Äù axons - chick interneurons labeled with green fluorescent protein that were transplanted from older into younger embryos.
 When they encounter unfamiliar territory, the axons create these trails as they try to recognize their pre-programmed trajectory. This image was first published in 
 Developmental Biology 297, 508-21. Honorable Mention, 2011 Olympus BioScapes Digital 
 Imaging Competition©.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
DOI: 10.6075/j0ww7gg6
title: CIL:41734, Gallus gallus. In Cell Image Library

description: B0008295. 2011 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://images.wellcome.ac.uk/indexplus/page/Prices.html
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label
Processing history: composite of two images
description: This image of the vascular system of a chicken embryo taken two days after fertilization is a composite of two different images taken with an upright fluorescent dissecting scope. The egg's shell was opened and fluorescent dextran was injected into the lower part of the vascular system. The heart pumped the dye and revealed the entire vasculature used by the embryo to feed itself from the rich underlying yolk inside the egg. Wellcome Image Award 2012.
DOI: 10.6075/j0vh5mms
title: CIL:41694, Homo sapiens, epidermoid carcinoma. In Cell Image Library

description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody 
 HPA030827 (shown in green). HPA030827 is a polyclonal anti-LRRC59 antibody that labels endoplasmic reticulum. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
DOI: 10.6075/j0513x30
title: CIL:41692, Homo sapiens, epidermoid carcinoma. In Cell Image Library

description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody 
 HPA028732 (shown in green). HPA028732 is a polyclonal anti-LAD1 antibody that labels intermediate filaments. The cells are also stained with a reference marker in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probe/organelle was used as reference; (i) DAPI for the nucleus (blue).
DOI: 10.6075/j0vd6z8s
title: CIL:37043, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

description: This is IMAGE #5 (time point: 6 hours)of an image group. It shows a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. In the series, z-stacks of this neuron were acquired at a 2 hour interval for 8 hours. Afterward, a second time lapse series was acquired at a 60 second interval for 10 minutes. This time series shows dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions. The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope
description: Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in: 
Ruthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603. 

The methods of cell transfection with electroporation can be found here: 
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272. 

Further explanation can be found here:
Bestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.

Bestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0qr4w6h
title: CIL:7665, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: High resolution detail of cytopharyngeal vesicles. TEM taken on 3/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j09k49gm
title: CIL:764, Patiria miniata, sperm. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; Low temperature embedding
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: modes of electron microscopy; EELS imaging
Source of contrast: electron scattering
Visualization methods: EELS window
Processing history: unprocessed raw data
description: Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 4 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 170 (this image), 160, 120, and 104eV.