DOI: 10.57760/sciencedb.agriculture.00281
title: Dataset of Sugar and Acid Components in 289 Table Grape Germplasms from the Hebei Grape Germplasm Repository
title: 河北省葡萄种质资源圃289种鲜食葡萄糖酸组分数据集

description: This study used 289 fresh grape germplasms from the Grape Germplasm Resources Garden in Hebei Province as materials to systematically analyze their fruit sugar and acid composition characteristics and genetic laws. The experimental samples were collected from the grape germplasm resource garden in Huailai County, Zhangjiakou City, Hebei Province from August to October 2023 and 2024 (fruit ripening period). Select three plants of consistent growth from each variety as biological replicates, and randomly collect 50 uniformly sized, mature, and disease-free fruits from each plant. After juicing the sample and centrifuging at 12000 rpm for 5 minutes at 10 ℃, the supernatant was taken and filtered through a 0.22 μ m nylon filter membrane. It was then divided into HPLC injection bottles and stored in a -40 ℃ ultra-low temperature freezer for testing. High performance liquid chromatography was used to determine the content of sugar and acid components separately. Determination of sugar components: The chromatographic system is Waters ™ Alliance， Equipped with a differential refractive index detector (2414). Use XBridge Amide chromatography column (4.6 mm × 250 mm, 3.5 μ m) and corresponding protective column. Using acetonitrile water (85:15, v/v) as the mobile phase, the column temperature and detector temperature were both 45 ℃, the flow rate was 1.5 mL/min, and the injection volume was 10 μ L. Acid component determination: Using the same chromatographic system, equipped with a UV detector (2489). The chromatographic column is Ultimate LP-C18 (4.6 × 300 mm, 5 μ m). Using a 0.01 mol/L potassium dihydrogen phosphate methanol (95:5, v/v) solution with pH 2.6 as the mobile phase, column temperature of 30 ℃, flow rate of 0.5 mL/min, detection wavelength of 210 nm, and injection volume of 10 μ L. Fructose, glucose, sucrose, oxalic acid, tartaric acid, and malic acid standards (purity ≥ 99.0%) were purchased from Dr. Ehrenstorfer company. Each standard sample is prepared with ultrapure water to form a series of gradient concentrations, and analyzed by injection under the above chromatographic conditions. Draw a standard curve with concentration as the x-axis and peak area as the y-axis. The standard curves of all target compounds showed good linearity (R ²&gt;0.99), and quantitative analysis of each component in the sample was performed based on this. 
description: This study used 289 fresh grape germplasms from the Grape Germplasm Resources Garden in Hebei Province as materials to systematically analyze their fruit sugar and acid composition characteristics and genetic laws. The experimental samples were collected from the grape germplasm resource garden in Huailai County, Zhangjiakou City, Hebei Province from August to October 2023 and 2024 (fruit ripening period). Select three plants of consistent growth from each variety as biological replicates, and randomly collect 50 uniformly sized, mature, and disease-free fruits from each plant. After juicing the sample and centrifuging at 12000 rpm for 5 minutes at 10 ℃, the supernatant was taken and filtered through a 0.22 μ m nylon filter membrane. It was then divided into HPLC injection bottles and stored in a -40 ℃ ultra-low temperature freezer for testing. High performance liquid chromatography was used to determine the content of sugar and acid components separately. Determination of sugar components: The chromatographic system is Waters ™ Alliance， Equipped with a differential refractive index detector (2414). Use XBridge Amide chromatography column (4.6 mm × 250 mm, 3.5 μ m) and corresponding protective column. Using acetonitrile water (85:15, v/v) as the mobile phase, the column temperature and detector temperature were both 45 ℃, the flow rate was 1.5 mL/min, and the injection volume was 10 μ L. Acid component determination: Using the same chromatographic system, equipped with a UV detector (2489). The chromatographic column is Ultimate LP-C18 (4.6 × 300 mm, 5 μ m). Using a 0.01 mol/L potassium dihydrogen phosphate methanol (95:5, v/v) solution with pH 2.6 as the mobile phase, column temperature of 30 ℃, flow rate of 0.5 mL/min, detection wavelength of 210 nm, and injection volume of 10 μ L. Fructose, glucose, sucrose, oxalic acid, tartaric acid, and malic acid standards (purity ≥ 99.0%) were purchased from Dr. Ehrenstorfer company. Each standard sample is prepared with ultrapure water to form a series of gradient concentrations, and analyzed by injection under the above chromatographic conditions. Draw a standard curve with concentration as the x-axis and peak area as the y-axis. The standard curves of all target compounds showed good linearity (R ²&gt;0.99), and quantitative analysis of each component in the sample was performed based on this.