DOI: 10.5061/dryad.5mkkwh7h4
title: Data from: Split genetically encoded calcium indicators for interorganellar junctions

description: This dataset describes the exact values used for some of the graphing in
 our investigation of Split genetically encoded calcium
 indicators. This dataset includes GraphPad Prism files used for graphing
 for each specified figure or a subset of a figure. These files mostly
 include post analysis fluorescence data for the different constructs used
 in our investigation. In addition raw video files of the 2-photon in-vivo
 recordings of split-sf-MEGIC2 expression are included. 
description: # Data from: Split genetically encoded calcium indicators for
 interorganellar junctions Dataset DOI:
 [10.5061/dryad.5mkkwh7h4](10.5061/dryad.5mkkwh7h4) ## Description of the
 data and file structure In brief, we have examined different genetically
 encoded calcium indicators constructs in-vitro in HEK293t cells and rat
 neurons, and in-vivo in mice and larval Zebrafish. All of the construct
 names (**GCaMP6s2**, **GCaMP6s3.0** and so on, marked in bold) are
 genetically encoded calcium indicators for which the exact structure and
 sequence can be found in the published article. The files are named as
 references to the figure/subset of a figure they are related to.\ The
 .pzfx files are GraphPad Prism files which contain the data used to graph.
 For all files and specifically those which reference multiple
 figures/subset of figures are also named and referenced by the associated
 figure/subset of figure inside the Prism files. The names of each
 construct used are also given in each file. Some aesthetical changes from
 the actual published article figures are present. The following are the
 result of analyzing fluorescence recordings of different calcium indicator
 constructs and represent the post analysis maximal ΔF/F (the maximal
 change in fluorescence recorded for each cell that expressed the
 construct): * Fig1b_j_Fig2c_g.pzfx :\ Fig1b - The ΔF/F
 of **GCaMP6s2**, **GCaMP6s3.0**, **GCaMP6s3.1** and **GCaMP6s3.2** 
 expressed in-vitro from rat neurons. Fig1j - The ΔF/F of **M13 cpGFP** and
 **M13 cpGFP + CaM** expressed in-vitro from rat neurons in soma and
 dendrites. Fig2c - The ΔF/F before and after ionomycin of activated
 **PA-sf-S2** and non-activated **PA-sf-S2** expressed in-vitro from
 HEK293t cells. Fig2g - The ΔF/F before and after ionomycin of
 **sfGCaMP6s~2~**, **osfGCaMP6s~2~** and **GCaMP6s2** expressed in-vitro
 from HEK293t cells. * Fig1g.pzfx - The ΔF/F of **M13-cpGFP and
 Calmodulin** expressed in soma and in dendrites in-vitro from rat neurons.
 * Fig3-b_c.pzfx - The ΔF/F of **GCaMP6s**, **Split-GCaMP6s3.0** and
 **Split-sf-S2** expressed in soma and in dendrites in-vitro from rat
 neurons. * fig4d.pzfx - The ΔF/F before and after Calcium addition of
 **sfGFP1–6 and sfGFP7–11**, and **sfGFP1–10 and sfGFP11** expressed
 in-vitro from HEK293t cells. * Fig5f-left.pzfx - The ΔF/F
 of **Split-sf-MEGIC~2~** in soma and in dendrites recorded in-vitro from
 rat neurons, including all events and then categorized by number of action
 potentials during each calcium transient. - Fig5h.pzfx - The ΔF/F of
 **Split-sf-MEGIC~2~** recorded in-vivo in larval Zebrafish. The following
 data also includes a decay time Tau~1/2~ analysis which represent how long
 it takes the fluorescence spike to decay (related to Figure 1). *
 Fig1g.pzfx - The decay time of **M13-cpGFP and Calmodulin** expressed
 in-vitro from rat neurons. The following data represents the basal
 fluorescence intensity recorded for each construct (related to Figure 4).
 * Fig4b.pzfx - The basal fluorescence intensity of **sfGFP1–6 and
 sfGFP7–11**, and **sfGFP1–10 and sfGFP11** expressed in-vitro from HEK293t
 cells. The following data represents Cross-Correlation calculations
 between neuronal spiking and each construct activation (related to Figure
 5). * Fig5f-right.pzfx - Cross-Correlation calculations between
 either **Split-sf-MEGIC2** or **GCaMP6s** *wt* activity with neuronal
 action potentials in-vitro from rat neurons.  The following are the raw
 2-photon recordings of **split-sf-MEGIC~2~** in mice brains in the primary
 motor cortex (related to Figure 7): * Fig7_FOV1_trial5.avi *
 Fig7_FOV2_trial2.avi * Fig7_FOV2_trial3.avi * Fig7_FOV2_trial4.avi *
 Fig7_FOV2_trial5.avi * Fig7_FOV2_trial6.avi * Fig7_FOV2_trial9.avi *
 Fig7_FOV4_trial1.avi * Fig7_FOV4_trial10.avi * Fig7_FOV4_trial11.avi *
 Fig7_FOV4_trial2.avi * Fig7_FOV4_trial3.avi * Fig7_FOV4_trial4.avi *
 Fig7_FOV4_trial5.avi * Fig7_FOV4_trial6.avi * Fig7_FOV4_trial7.avi *
 Fig7_FOV4_trial8.avi * Fig7_FOV4_trial9.avi ## Code/software GraphPad
 Prism 8. ## Access information Other publicly accessible locations of the
 data: * None. Data was derived from the following sources: * Data was not
 derived from other sources.