cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j08w3dg8
title: CIL:50672, Drosophila melanogaster. In Cell Image Library

description: Item type: Digital
Imaging mode: CM12 TEM equipped with AMT digital camera
description: Specimen preparation: Larval brains (LBs) were prepared from starved (amino acid-restricted) first instar (L1) larvae to enrich for quiescent neural stem cells. The LBs were glutaraldehyde-fixed, tannic acid and OsO4-stained, embedded in resin, thin-sectioned (60-70 nm) and stained with uranyl acetate/lead citrate, then observed by TEM.
description: We show in this study that brain and intestinal stem cells of Drosophila contain a new type of cytoplasmic protrusion which is enriched in mitochondria and that the mitochondria are probably dependent on microtubules for their distribution. The protrusions form mitochondria-enriched cytoplasmic bridges from the stem cells to their niche that we speculate are important in regulating stem cell quiescence and activation.
DOI: 10.6075/j0ms3s12
title: CIL:579, Gallus gallus gallus, erythrocyte. In Cell Image Library

description: Avian erythrocytes differ from most vertebrates in retaining the nucleus at maturity. During erythrocyte development, transcription is progressively shut down and nuclei become compact and heterochromatic. Since erythrocyte chromatin contains little other than histones and DNA, it is the material of choice for many in vivo and in vitro studies.
This image, recorded on film at 100KV is a thin section, prepared from washed blood fixed with glutaraldehye, post-fixed with OsO4, embedded in plastic and stained with uranyl and lead salts. The dark, centrally located nucleus is prominent, and the chromatin so compact that the chromatin fiber organization is obscured.
description: Preparation: glutaraldehyde fixed tissue; microtome-sectioned tissue
Relation to intact cell: tissue in plastic embedment
Item type: recorded image; film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: inelastic scattering
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
DOI: 10.6075/j0z31xwc
title: CIL:578, Gallus gallus gallus, erythrocyte. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; microtome-sectioned tissue
Relation to intact cell: tissue in plastic embedment
Item type: recorded image; film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: inelastic scattering
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
description: Avian erythrocytes differ from most vertebrates in retaining the nucleus at maturity. During erythrocyte development, transcription is progressively shut down and nuclei become compact and heterochromatic. Since erythrocyte chromatin contains little other than histones and DNA, it is the material of choice for many in vivo and in vitro studies.
This image, recorded on film at 100KV is a thin section, prepared from washed blood fixed with glutaraldehye, post-fixed with OsO4, embedded in plastic and stained with uranyl and lead salts. The dark, centrally located nucleus is prominent, and the chromatin so compact that the chromatin fiber organization is obscured.
DOI: 10.6075/j09z9456
title: CIL:571, Acetabularia mediterranea, gamete. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data
description: Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.
description: At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium is being divided into uninuclear gametes, and the formation of flagella from paired centrioles is in progress. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.
DOI: 10.6075/j0x929h4
title: CIL:561, Acetabularia mediterranea, gamete. In Cell Image Library

description: At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium has just begun to divide into uninuclear gametes. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.
description: Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.
description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data
DOI: 10.15125/bath-01612
title: Figures for publication 'Six-component electromagnetic wave measurements of sprite-associated lightning'

description: This data is to illustrate the theory, data analysis, and exemplary results of the data described in the corresponding publication entitled 'Six-component electromagnetic wave measurements of sprite-associated lightning'. 
Low frequency electromagnetic waves emitted by sprite-producing lightning are normally measured using vertical electric fields or horizontal magnetic fields. Here we report for the first time the simultaneous measurement of electromagnetic waves from sprite-producing lightning in all six electromagnetic field components Ex, Ey, Ez, Hx, Hy, and Hz. A rigorous assessment of the horizontal electric field measurements with dipole antennas in two independent calibration experiments shows that a timing uncertainty of ∼ 1−2 ns can be achieved, well above the current fundamental limit of the timing accuracy ∼1-5 ps. The coupling between the electric and magnetic fields is quantified using a transfer matrix, allowing the magnetic field to be reconstructed accurately from electric field measurements. The cross product of electric and magnetic fields is used to calculate peak energy fluxes and arrival azimuths from sprite-producing lightning. It is found that peak energy fluxes vary between ∼10-1000 µW/m 2 and that the differences between the measured and expected arrival azimuths are practically normally distributed with a mean and standard deviation of -7.0 deg ± 2.4 deg. It is concluded that horizontal electric field measurements are well suited to characterise electromagnetic waves with added benefits, including the ease of deployment in harsh environments, cost-effectiveness and scalability, e.g. for polarisation measurements in large low frequency arrays. The significance of this study is that it can be used as a pathfinder mission to identify critical technical requirements for the array deployment during the Africa2Moon lander mission.
description: This data lives in the context of the corresponding publication entitled 'Six-component electromagnetic wave measurements of sprite-associated lightning'. Any use of the data outside this specific context is considered to be unethical. 
The data is described in three different forms that build on each other in a logical and hierarchical order. 
 (1) High level description: All the data is described in textual form in the corresponding publication in extensive detail. 
 (2) Low level description: The actual values of the data and their units which are used in the publication are embedded in the corresponding standard Matlab .fig files, one for each Figure.
description: The Matlab programming language is explained on the website https://uk.mathworks.com/help/matlab/getting-started-with-matlab.html?s_cid=learn_doc.
DOI: 10.6075/j0n29w6w
title: CIL:552, Acetabularia mediterranea, gamete. In Cell Image Library

description: At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium is being divided into uninuclear gametes, and the formation of flagella from paired centrioles is in progress. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.
description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data
description: Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.
DOI: 10.6075/j0h1318k
title: CIL:551, Acetabularia mediterranea, gamete. In Cell Image Library

description: At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium is being divided into uninuclear gametes, and the formation of flagella from paired centrioles is in progress. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.
description: Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.
description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data
DOI: 10.6075/j0cn742m
title: CIL:50681, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library

description: Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. The serial EM data was collected at 15 nm milling step, and pixel resolution within the range of 1.95-2.75 nm/pixel.
description: Imaging mode: Serial electron microscopy; focused ion beam scanning electron mictoscopy (FIB-SEM)
Visualization methods: Heavy metal stain (osmium tetroxide, potassium ferrocyanide, tannic acid), immunogold labeling using 10 nm gold beads
description: Postnatal day 4 mouse inner ear outer hair cell stereocilia bundle. Sample was labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset also includes the apical end of the hair cell.
DOI: 10.15468/dl.cvqdz8
title: Occurrence Download

description: A dataset containing 223 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Nepal",
 "OccurrenceStatus is Present",
 "TaxonKey is Axis porcinus"
 ]
}

 The dataset includes 223 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009736-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0p55np3
title: CIL:48603, Mus musculus, parietal cell, zymogen, mucus. In Cell Image Library

description: Preparation: Glutaraldehyde and paraformaldehyde in phosphate buffer
Stain2: Toluidine blue
Embed: Spurr's resin
Section: 1 micron
Magnification: 100 x oil
Species: musculus (mixed 129SvJ and Black Swiss)
Age: 61 days
Subspecies or gene: gastric, H,K, aATPase alpha -/-
Organ: stomach
Tissue: gastric mucosa, greater curvature
description: Item type: micrograph
Imaging mode: modes of light microscopy
description: Light micrograph, toluidine blue staining
DOI: 10.6075/j0td9w5f
title: CIL:41636, Physarum leucophaeum, spore. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
description: Fluorescent image of the sporangium, an enclosure in which spores are formed, of the slime mold Physarum leucophaeum. Honorable Mention, 2011 Olympus BioScapes Digital Imaging Competition©
DOI: 10.6075/j0m61j03
title: CIL:12224, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous CENP-A is green and DAPI (DNA) is blue. See Supplementary Fig 3B panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j03b5xwj
title: CIL:12223, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous Aurora B is green and DAPI (DNA) is blue. See Supplementary Fig 3C panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0765d2f
title: CIL:12222, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous CENP-A is green and DAPI (DNA) is blue. See Supplementary Fig 3D panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0pz57j8
title: CIL:12204, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2D panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j04q7srz
title: CIL:12203, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2A panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0dv1hmf
title: CIL:12245, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of CENP-E(green)in INCENP-ON cells. Alpha-tubulin is red and DAPI (DNA) is blue. See Supplementary Fig 1A panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0x9291v
title: CIL:12221, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous CENP-A is green and DAPI (DNA) is blue. See Supplementary Fig 3D panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0kp80w9
title: CIL:12220, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase in INCENP-OFF cells expressing TrAP-INCENP WT. Histone H3 modification H3-T3ph shown in red and H3-S28ph in green. DAPI (DNA) is blue. See Supplementary Fig 3E panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j09z93mm
title: CIL:12217, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase in INCENP-OFF cells expressing TrAP-INCENP WT. Histone H3 modification H3-S10ph shown in green. DAPI (DNA) is blue. See Supplementary Fig 3E panels 5-7 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed