DOI: 10.5061/dryad.bp6714t
title: Data from: Hybridization rate and hybrid fitness for Camelina microcarpa Andrz. ex DC (♀) and Camelina sativa (L.) Crantz(Brassicaceae) (♂)

description: Hybridization between crops and their wild relatives has the potential to
 introduce novel variation into wild populations. Camelina (Camelina
 sativa) is a promising oilseed and cultivars with modified seed
 characteristics and herbicide resistance are in development, prompting a
 need to evaluate the potential for novel trait introgression into weedy
 relatives. Little-podded false flax (littlepod; Camelina microcarpa) is a
 naturalized weed in Canada and the USA. Here we evaluated the
 hybridization rate between the three cytotypes of littlepod (♀) and
 camelina (♂), assessed characteristics of hybrids, and evaluated the
 fitness of hexaploid littlepod and camelina hybrids in the glasshouse and
 field. In total we conducted, 1,005 manual crosses with diploid littlepod,
 1, 172 crosses with tetraploid littlepod, and 896 crosses with hexaploid
 littlepod. Hybrids were not produced by the diploids, but were produced by
 the tetraploids and hexaploids at rates of 1 hybrid for 2,000 ovules
 pollinated and 24 hybrids for 25 ovules pollinated, respectively. Hybrids
 between tetraploid littlepod and camelina showed low pollen fertility and
 produced a small number of seeds. In the glasshouse, hybrids between
 hexaploid littlepod and camelina also showed significantly lower pollen
 fertility and seed production than parental lines, but their seeds showed
 high viability. A similar pattern was observed in field trials, with
 hybrids showing earlier flowering, reduced biomass, seed production and
 seed weight. However, seed produced by the hybrids showed greater
 viability than that produced by hexaploid littlepod and is potentially the
 result of a shortened lifecycle. The introgression of lifecycle traits
 into littlepod populations may facilitate range expansion and contribute
 to crop gene persistence. Consequently, future work should evaluate the
 hybridization rate in the field, the fitness of advanced generation
 backcrosses, and the role of time to maturity in limiting hexaploid
 littlepod’s distribution.
description: Camelina microcarpa
 diploid and Camelina sativa crosses Mar 12 2018Information on the seed
 use and crosses completed between between Camelina microcarpa (female, 2n
 = 12) and Camelina sativa (male, 2n = 40).Camelina microcarpa
 tetraploid and Camelina sativa hybrid data March 12 2018Information on seed
 sources, crossing work and characterization of the morphology and
 fertility of hybrids reared in the green house for crosses between
 Camelina microcarpa (2n = 26; female) and Camelina sativa (2n = 40; male).
 Metadata on contents is included in the first sheet of the
 workbook.Camelina microcarpa
 hexaploid and Camelina sativa hybrid data March 12 2018Information on seed
 sources, crossing work and characterization of the morphology and
 fertility of hybrids reared in the green house and field for crosses
 between Camelina microcarpa (2n = 40; female) and Camelina sativa (2n =
 40; male). Metadata on contents is included in the first sheet of the
 workbook.