cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.7910/dvn/qcnyed/tw8ouj
title: gep16.t00z.pgrb2af06.cycfs12.idx

description: :unav
DOI: 10.7910/dvn/x9gtyf/ikkbap
title: gep18.t00z.pgrb2bf30.idx

description: :unav
DOI: 10.7910/dvn/xncfct/shunc0
title: gep17.t00z.pgrb2af222

description: :unav
DOI: 10.7910/dvn/qcnyed/vcgla3
title: gep16.t00z.pgrb2af198

description: :unav
DOI: 10.7910/dvn/x9gtyf/b6p7kl
title: gep18.t00z.pgrb2bf366.idx

description: :unav
DOI: 10.7910/dvn/xncfct/9ur4jc
title: gep17.t00z.pgrb2bf156.idx

description: :unav
DOI: 10.7910/dvn/qcnyed/t6urol
title: gep16.t00z.pgrb2bf54.idx

description: :unav
DOI: 10.7910/dvn/x9gtyf/9sszsq
title: gep18.t00z.pgrb2bf228.idx

description: :unav
DOI: 10.7910/dvn/x9gtyf/ey70oj
title: gep18.t00z.pgrb2bf312

description: :unav
DOI: 10.7910/dvn/xncfct/didolb
title: gep17.t00z.pgrb2bf54

description: :unav
DOI: 10.7910/dvn/qcnyed/iulimt
title: gep16.t00z.pgrb2aanl.cycfs06.idx

description: :unav
DOI: 10.7910/dvn/x9gtyf/s5d5vf
title: gep18.t00z.pgrb2bf168.idx

description: :unav
DOI: 10.7910/dvn/xncfct/x9jitd
title: gep17.t00z.pgrb2af00.cycfs12

description: :unav
DOI: 10.7910/dvn/qcnyed/cuzrfi
title: gep16.t00z.pgrb2af378

description: :unav
DOI: 10.7910/dvn/x9gtyf/pkldds
title: gep18.t00z.pgrb2af90

description: :unav
DOI: 10.7910/dvn/xncfct/lkmfky
title: gep17.t00z.pgrb2bf24.idx

description: :unav
DOI: 10.7910/dvn/qcnyed/mrvocw
title: gep16.t00z.pgrb2af318

description: :unav
DOI: 10.7910/dvn/x9gtyf/fk8cp9
title: gep18.t00z.pgrb2bf66.idx

description: :unav
DOI: 10.7910/dvn/xncfct/bd16md
title: gep17.t00z.pgrb2af234

description: :unav
DOI: 10.7910/dvn/qcnyed/levxtc
title: gep16.t00z.pgrb2bf210

description: :unav
DOI: 10.7910/dvn/x9gtyf/umoxzw
title: gep18.t00z.pgrb2aanl.cycfs06.idx

description: :unav
DOI: 10.7910/dvn/xncfct/uym1ik
title: gep17.t00z.pgrb2bf216

description: :unav
DOI: 10.7910/dvn/xncfct/9efs8k
title: gep17.t00z.pgrb2bf06

description: :unav
DOI: 10.7910/dvn/x9gtyf/i0npec
title: gep18.t00z.pgrb2af336

description: :unav
DOI: 10.7910/dvn/qcnyed/zzhsaj
title: gep16.t00z.pgrb2bf00.idx

description: :unav
DOI: 10.7910/dvn/xncfct/aovrm4
title: gep17.t00z.pgrb2bf126.idx

description: :unav
DOI: 10.7910/dvn/xncfct/xme5h6
title: gep17.t00z.pgrb2af06

description: :unav
DOI: 10.7910/dvn/qcnyed/izdbp2
title: gep16.t00z.pgrb2af342

description: :unav
DOI: 10.7910/dvn/x9gtyf/5dduyu
title: gep18.t00z.pgrb2bf42.idx

description: :unav
DOI: 10.7910/dvn/xncfct/5c8uj5
title: gep17.t00z.pgrb2af288.idx

description: :unav
DOI: 10.7910/dvn/qcnyed/djkpon
title: gep16.t00z.pgrb2af18

description: :unav
DOI: 10.7910/dvn/x9gtyf/rcc4gz
title: gep18.t00z.pgrb2af204.idx

description: :unav
DOI: 10.7910/dvn/qcnyed/xiunpx
title: gep16.t00z.pgrb2af216

description: :unav
DOI: 10.7910/dvn/xncfct/tksay8
title: gep17.t00z.pgrb2af276.idx

description: :unav
DOI: 10.7910/dvn/x9gtyf/zyntst
title: gep18.t00z.pgrb2af234

description: :unav
DOI: 10.7910/dvn/xncfct/ycz5dh
title: gep17.t00z.pgrb2bf228.idx

description: :unav
DOI: 10.7910/dvn/qcnyed/hitaiw
title: gep16.t00z.pgrb2bf198.idx

description: :unav
DOI: 10.7910/dvn/x9gtyf/1vy1l0
title: gep18.t00z.pgrb2af342

description: :unav
DOI: 10.7910/dvn/xncfct/p1jyq6
title: gep17.t00z.pgrb2af12.idx

description: :unav
DOI: 10.7910/dvn/qcnyed/oqiovc
title: gep16.t00z.pgrb2bf00

description: :unav
DOI: 10.22002/1x06v-e9x47
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-284/rawdata/</p>Caulobacter333_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-284/rawdata/Caulobacter333_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter333_movies_part56_1.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-284/3dimage_116496/Caulobacter333_movies_part56_1.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter333.mrc.mdoc,  40.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-284/rawdata/Caulobacter333.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cnq8m-ah214
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-135/rawdata/</p>Caulobacter147_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-135/rawdata/Caulobacter147_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter147_movies_part55_3.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-135/3dimage_116186/Caulobacter147_movies_part55_3.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter147.mrc.mdoc,  39.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-135/rawdata/Caulobacter147.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bz9q7-jhf92
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-273/rawdata/</p>Caulobacter323_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-273/rawdata/Caulobacter323_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter323_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-273/3dimage_116452/Caulobacter323_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter323.mrc.mdoc,  16.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-273/rawdata/Caulobacter323.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/63vrt-b1m68
title: WT tomogram data

description: Tad pilus
description: <strong>Tilt Series Date:</strong> 2020-09-10</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-09-10-9/rawdata/</p>200910_Caulobacter_1_3_10_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-09-10-9/rawdata/200910_Caulobacter_1_3_10_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        200910_Caulobacter_1_3_10_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-09-10-9/3dimage_113439/200910_Caulobacter_1_3_10_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        200910_Caulobacter_1_3_10_movies.mrc.mdoc,  41.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-09-10-9/rawdata/200910_Caulobacter_1_3_10_movies.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q5v3y-33v71
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-208/rawdata/</p>Caulobacter220_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-208/rawdata/Caulobacter220_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter220_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-208/3dimage_116331/Caulobacter220_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter220.mrc.mdoc,  44.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-208/rawdata/Caulobacter220.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0n9wz-13k94
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-176/rawdata/</p>Caulobacter188_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-176/rawdata/Caulobacter188_movies.mrc"
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        Caulobacter188_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
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        Caulobacter188.mrc.mdoc,  44.2 kB  
        
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