cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19597094.v1
title: Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284

description: Long non-coding RNAs (lncRNAs) have been widely studied and play crucial roles in cervical cancer (CC) progression. Here, we investigated the function and mechanism of lncRNA PGM5-AS1 action in CC cells. Using real-time quantitative polymerase chain reaction or western blotting, PGM5-AS1 and decorin (DCN) were downregulated in CC tissues and cells, whereas miR-4284 was upregulated. Luciferase assay, RNA pull-down assay, and western blotting showed that PGM5-AS1 could sponge miR-4284 to upregulate DCN expression in CC cells. Additionally, cell functional experiments showed that PGM5-AS1 overexpression led to decreased proliferation, migration, and invasion of CC cells. However, the inhibitory effect of PGM5-AS1 overexpression on CC cells was partly relieved by DCN knockdown because of the targeting interaction between PGM5-AS1, miR-4284, and DCN. In summary, this study identified that PGM5-AS1 negatively regulates CC cell malignancy by targeting miR-4284/DCN.
DOI: 10.6084/m9.figshare.19619731
title: MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1 alpha

description: Lung adenocarcinoma (LUAD) severely threatens the health of people owing to its lethality. Nonetheless, the underlying mechanisms on LUAD development remain unclear to a great extent. This work aimed to probe the functions of miR-218-5p in LUAD. MiR-218-5p and endoplasmic reticulum oxidoreductase 1 alpha (ERO1A) were screened as differently downregulated and upregulated RNAs in LUAD, respectively, by bioinformatics analyses. The results of cell functional assays stated that enforced expression of miR-218-5p notably restrained cell viability, invasion, and migration in LUAD. MiR-218-5p may interact with 3’-untranslated region of ERO1A mRNA as analyzed by bioinformatics. Afterward, western blot and dual-luciferase reporter gene analyses were introduced to identify their interaction. ERO1A overexpression reversed the suppressive impacts of miR-218-5p on LUAD cell progression, indicating the implication of miR-218-5p/ERO1A axis in suppressing cancer development. We also observed that this regulatory axis suppressed angiogenesis in LUAD. Taken together, miR-218-5p/ERO1A axis exerted an imperative role in LUAD cell progression, which provides a valuable clue for the development of LUAD therapeutic regimen.
DOI: 10.6084/m9.figshare.19619731.v1
title: MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1 alpha

description: Lung adenocarcinoma (LUAD) severely threatens the health of people owing to its lethality. Nonetheless, the underlying mechanisms on LUAD development remain unclear to a great extent. This work aimed to probe the functions of miR-218-5p in LUAD. MiR-218-5p and endoplasmic reticulum oxidoreductase 1 alpha (ERO1A) were screened as differently downregulated and upregulated RNAs in LUAD, respectively, by bioinformatics analyses. The results of cell functional assays stated that enforced expression of miR-218-5p notably restrained cell viability, invasion, and migration in LUAD. MiR-218-5p may interact with 3’-untranslated region of ERO1A mRNA as analyzed by bioinformatics. Afterward, western blot and dual-luciferase reporter gene analyses were introduced to identify their interaction. ERO1A overexpression reversed the suppressive impacts of miR-218-5p on LUAD cell progression, indicating the implication of miR-218-5p/ERO1A axis in suppressing cancer development. We also observed that this regulatory axis suppressed angiogenesis in LUAD. Taken together, miR-218-5p/ERO1A axis exerted an imperative role in LUAD cell progression, which provides a valuable clue for the development of LUAD therapeutic regimen.
DOI: 10.6084/m9.figshare.19367906
title: Magnesium isoglycyrrhizinate suppresses bladder cancer progression by modulating the miR-26b/Nox4 axis

description: Magnesium isoglycyrrhizinate (MI), a magnesium salt of 18α-GA stereoisomer, has been reported to exert efficient hepatoprotective activity. However, its effect on bladder cancer remains unclear. The study explored the effects of MI on the growth, colony formation, apoptosis, invasion, and migration of bladder cancer cells (HTB9 and BIU87 cells). Typical apoptotic changes of bladder cancer cells such as nuclear concentration and fragmentation were observed using Hoechst staining. The effects of MI on the expression levels of microRNA-26b (miR-26b), NADPH oxidase 4 (Nox4), nuclear transcription factor-κB (NF-κB), and hHypoxia inducible factor-1α (HIF-1α) were detected using qRT-PCR and Western blot. The potential targets of miR-26b were predicted using Targetscan, and their interactions were determined by luciferase reporter assay. A xenograft mouse model was established to evaluate the anti-tumor effects of MI <i>in vivo</i>. MI significantly suppressed the proliferation, colony formation, invasion, and migration and induced apoptosis of human bladder cancer cells, and MI significantly increased miR-26b expression. Nox 4 was identified to be a direct target of miR-26b. MiR-26b mimics significantly decreased the relative luciferase activity of wild type (WT) Nox 4 but not mutant type (MUT) Nox4. Meanwhile, MI markedly downregulated the expression levels of Nox4, NF-κB, and HIF-1α both <i>in vitro</i> and <i>in vivo</i>. Moreover, MI inhibited xenograft tumor growth <i>in vivo</i> and decreased the expression of Nox4, NF-κB, and HIF-1α. Overall, MI showed a potent anti-tumor effect against bladder cancer partially via modulating the miR-26b/Nox4 axis.
DOI: 10.6084/m9.figshare.19391051
title: Nuclear receptor 4A1 (NR4A1) silencing protects hepatocyte against hypoxia-reperfusion injury <i>in vitro</i> by activating liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling

description: The nuclear receptor 4A1 (NR4A1) is widely involved in the regulation of cell survival and is related to ischemic injury in several organs. This research examined the emerging role and mechanism of NR4A1 in hepatocyte ischemia-reperfusion injury (IRI). BRL-3A cells were subjected to hypoxia-reperfusion (H/R) to simulate an IRI model <i>in vitro</i>. The expression of NR4A1 and liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) pathway-related proteins (LKB1, AMPK, and ACC) was detected by western blotting or RT-qPCR under H/R condition after NR4A1 overexpression or silencing. Then, radicicol, an inhibitor of LKB1 pathway, was used to determine the role of NR4A1 in hepatocyte H/R injury by regulating LKB1. Under the help of CCK-8 assay, cell viability was assessed. The levels of ROS, MDA, and SOD were determined with corresponding kits to evaluate oxidative stress. Additionally, RT-qPCR was employed to analyze the releases of the inflammatory factors. Flow cytometry was applied to estimate the apoptosis and its related proteins, and autophagy-associated proteins were assayed by western blotting. Results indicated that NR4A1 was highly expressed, while proteins in LKB1/AMPK signaling was downregulated in BRL-3A cells exposed to H/R. The activation of LKB1/AMPK pathway could be negatively regulated by NR4A1. Moreover, NR4A1 depletion conspicuously promoted cell viability, inhibited oxidative stress as well as inflammation, and induced apoptosis and autophagy in H/R-stimulated BRL-3A cells, which were reversed after radicicol intervention. Collectively, NR4A1/LKB1/AMPK axis is a new protective pathway involved in hepatocyte IRI, shedding new insights into the improvement of hepatocyte IRI.
DOI: 10.6084/m9.figshare.19391051.v1
title: Nuclear receptor 4A1 (NR4A1) silencing protects hepatocyte against hypoxia-reperfusion injury <i>in vitro</i> by activating liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling

description: The nuclear receptor 4A1 (NR4A1) is widely involved in the regulation of cell survival and is related to ischemic injury in several organs. This research examined the emerging role and mechanism of NR4A1 in hepatocyte ischemia-reperfusion injury (IRI). BRL-3A cells were subjected to hypoxia-reperfusion (H/R) to simulate an IRI model <i>in vitro</i>. The expression of NR4A1 and liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) pathway-related proteins (LKB1, AMPK, and ACC) was detected by western blotting or RT-qPCR under H/R condition after NR4A1 overexpression or silencing. Then, radicicol, an inhibitor of LKB1 pathway, was used to determine the role of NR4A1 in hepatocyte H/R injury by regulating LKB1. Under the help of CCK-8 assay, cell viability was assessed. The levels of ROS, MDA, and SOD were determined with corresponding kits to evaluate oxidative stress. Additionally, RT-qPCR was employed to analyze the releases of the inflammatory factors. Flow cytometry was applied to estimate the apoptosis and its related proteins, and autophagy-associated proteins were assayed by western blotting. Results indicated that NR4A1 was highly expressed, while proteins in LKB1/AMPK signaling was downregulated in BRL-3A cells exposed to H/R. The activation of LKB1/AMPK pathway could be negatively regulated by NR4A1. Moreover, NR4A1 depletion conspicuously promoted cell viability, inhibited oxidative stress as well as inflammation, and induced apoptosis and autophagy in H/R-stimulated BRL-3A cells, which were reversed after radicicol intervention. Collectively, NR4A1/LKB1/AMPK axis is a new protective pathway involved in hepatocyte IRI, shedding new insights into the improvement of hepatocyte IRI.
DOI: 10.6084/m9.figshare.25357198
title: Data of "Testing sea urchin and green sea turtle consumption of the allelopathic macroalga <i>Galaxaura divaricata</i>"

description: This dataset documents the feeding ratios of the green sea turtle (<i>Chelonia mydas</i>) and five species of sea urchins (<i>Diadema setosum</i>, <i>Echinometra mathaei</i>, <i>Diadema savignyi</i>, <i>Echinothrix calamaris</i>, and <i>Tripneustes gratilla</i>) on two different red macroalgae (<i>Galaxaura divaricata</i> and <i>Gracilaria edulis</i>) conducted in the Dongsha Atoll Research Station during the summer of 2016. In experiments targeting green sea turtles as grazers, each individual green sea turtle was separately tested for a) feeding exclusively on <i>Galaxaura divaricata</i>, b) feeding exclusively on <i>Gracilaria edulis</i>, c) feeding simultaneously on a mixed diet of both red macroalgae species, and d) feeding simultaneously on an unmixed diet of both red macroalgae species. In experiments focusing on sea urchins as grazers, each sea urchin individual was tested separately for its feeding ratio on a) <i>Galaxaura divaricata</i> only, b) <i>Gracilaria edulis</i> only, and c) on both of these two red macroalgae simultaneously. We calculated the intake ratios based on weight changes of red macroalgae. The R codes for model fittings are provided. All details were documented in the article "Testing sea urchin and green sea turtle consumption of the allelopathic macroalga <i>Galaxaura divaricata</i>" by Nieder et al.
DOI: 10.6084/m9.figshare.25357198.v2
title: Data of "Testing sea urchin and green sea turtle consumption of the allelopathic macroalga <i>Galaxaura divaricata</i>"

description: This dataset documents the feeding ratios of the green sea turtle (<i>Chelonia mydas</i>) and five species of sea urchins (<i>Diadema setosum</i>, <i>Echinometra mathaei</i>, <i>Diadema savignyi</i>, <i>Echinothrix calamaris</i>, and <i>Tripneustes gratilla</i>) on two different red macroalgae (<i>Galaxaura divaricata</i> and <i>Gracilaria edulis</i>) conducted in the Dongsha Atoll Research Station during the summer of 2016. In experiments targeting green sea turtles as grazers, each individual green sea turtle was separately tested for a) feeding exclusively on <i>Galaxaura divaricata</i>, b) feeding exclusively on <i>Gracilaria edulis</i>, c) feeding simultaneously on a mixed diet of both red macroalgae species, and d) feeding simultaneously on an unmixed diet of both red macroalgae species. In experiments focusing on sea urchins as grazers, each sea urchin individual was tested separately for its feeding ratio on a) <i>Galaxaura divaricata</i> only, b) <i>Gracilaria edulis</i> only, and c) on both of these two red macroalgae simultaneously. We calculated the intake ratios based on weight changes of red macroalgae. The R codes for model fittings are provided. All details were documented in the article "Testing sea urchin and green sea turtle consumption of the allelopathic macroalga <i>Galaxaura divaricata</i>" by Nieder et al.
DOI: 10.6084/m9.figshare.25357198.v1
title: Data of "Testing sea urchin and green sea turtle consumption of the allelopathic macroalga <i>Galaxaura divaricata</i>"

description: This dataset documents the feeding ratios of the green sea turtle (<i>Chelonia mydas</i>) and five species of sea urchins (<i>Diadema setosum</i>, <i>Echinometra mathaei</i>, <i>Diadema savignyi</i>, <i>Echinothrix calamaris</i>, and <i>Tripneustes gratilla</i>) on two different red macroalgae (<i>Galaxaura divaricata</i> and <i>Gracilaria edulis</i>) conducted in the Dongsha Atoll Research Station during the summer of 2016. In experiments targeting green sea turtles as grazers, each individual green sea turtle was separately tested for a) feeding exclusively on <i>Galaxaura divaricata</i>, b) feeding exclusively on <i>Gracilaria edulis</i>, c) feeding simultaneously on a mixed diet of both red macroalgae species, and d) feeding simultaneously on an unmixed diet of both red macroalgae species. In experiments focusing on sea urchins as grazers, each sea urchin individual was tested separately for its feeding ratio on a) <i>Galaxaura divaricata</i> only, b) <i>Gracilaria edulis</i> only, and c) on both of these two red macroalgae simultaneously. We calculated the intake ratios based on weight changes of red macroalgae. The R codes for model fittings are provided. All details were documented in the article "Testing sea urchin and green sea turtle consumption of the allelopathic macroalga <i>Galaxaura divaricata</i>" by Nieder et al.
DOI: 10.4121/22ade4b5-9b45-40a1-b526-a91f4c2a0336
title: Data underlying the publication: Wall shear stress measurement using a zero-displacement floating-element balance

description: The dataset includes direct measurements of the skin friction coefficient of the smooth-wall boundary layer using a zero-displacement floating element (FE) balance and oil film interferometry (OFI). The corresponding mean velocity profiles from hot-wire anemometry are also provided. The measurements cover a wide range of momentum thickness Reynolds numbers from 8800 to 35300. The experiment was designed to assess the performance of the newly developed FE balance, with OFI serving as the benchmark. For a detailed description of the experimental methods refer to Ferreira, M.A., Costa, P., Ganapathisubramani, G. (2016) Wall shear stress measurement using a zero-displacement floating-element balance. Exp. Fluids 57:90. https://doi.org/10.1007/s00348-024-03785-1.
DOI: 10.4121/22ade4b5-9b45-40a1-b526-a91f4c2a0336.v1
title: Data underlying the publication: Wall shear stress measurement using a zero-displacement floating-element balance

description: The dataset includes direct measurements of the skin friction coefficient of the smooth-wall boundary layer using a zero-displacement floating element (FE) balance and oil film interferometry (OFI). The corresponding mean velocity profiles from hot-wire anemometry are also provided. The measurements cover a wide range of momentum thickness Reynolds numbers from 8800 to 35300. The experiment was designed to assess the performance of the newly developed FE balance, with OFI serving as the benchmark. For a detailed description of the experimental methods refer to Ferreira, M.A., Costa, P., Ganapathisubramani, G. (2016) Wall shear stress measurement using a zero-displacement floating-element balance. Exp. Fluids 57:90. https://doi.org/10.1007/s00348-024-03785-1.
DOI: 10.5281/zenodo.10852752
title: MIC dataset for Anisaxin WP5 Task 2

description: Data from an MIC experiment using Edwardsiella and othe bacteria with anisaxin
DOI: 10.5281/zenodo.10852753
title: MIC dataset for Anisaxin WP5 Task 2

description: Data from an MIC experiment using Edwardsiella and othe bacteria with anisaxin
DOI: 10.5281/zenodo.5500433
title: MeshRIR: Dataset of Room Impulse Responses on Meshed Grid Points

description: MeshRIR is a dataset of acoustic room impulse responses (RIRs) at finely meshed grid points. Two subdatasets are currently available: one consists of IRs in a 3D cuboidal region from a single source, and the other consists of IRs in a 2D square region from an array of 32 sources. This dataset is suitable for evaluating sound field analysis and synthesis methods. See the link below for the details: https://sh01k.github.io/MeshRIR/
DOI: 10.5281/zenodo.10852693
title: MeshRIR: Dataset of Room Impulse Responses on Meshed Grid Points

description: MeshRIR is a dataset of acoustic room impulse responses (RIRs) at finely meshed grid points. Two subdatasets are currently available: one consists of IRs in a 3D cuboidal region from a single source, and the other consists of IRs in a 2D square region from an array of 32 sources. This dataset is suitable for evaluating sound field analysis and synthesis methods. 

See the link below for the details:

https://sh01k.github.io/MeshRIR/
DOI: 10.5281/zenodo.5002817
title: MeshRIR: Dataset of Room Impulse Responses on Meshed Grid Points

description: MeshRIR is a dataset of acoustic room impulse responses (RIRs) at finely meshed grid points. Two subdatasets are currently available: one consists of IRs in a 3D cuboidal region from a single source, and the other consists of IRs in a 2D square region from an array of 32 sources. This dataset is suitable for evaluating sound field analysis and synthesis methods. 

See the link below for the details:

https://sh01k.github.io/MeshRIR/
DOI: 10.5281/zenodo.5002818
title: MeshRIR: Dataset of Room Impulse Responses on Meshed Grid Points

description: MeshRIR is a dataset of acoustic room impulse responses (RIRs) at finely meshed grid points. Two subdatasets are currently available: one consists of IRs in a 3D cuboidal region from a single source, and the other consists of IRs in a 2D square region from an array of 32 sources. This dataset is suitable for evaluating sound field analysis and synthesis methods. See the link below for the details: https://sh01k.github.io/MeshRIR/
DOI: 10.5281/zenodo.5500451
title: MeshRIR: Dataset of Room Impulse Responses on Meshed Grid Points

description: MeshRIR is a dataset of acoustic room impulse responses (RIRs) at finely meshed grid points. Two subdatasets are currently available: one consists of IRs in a 3D cuboidal region from a single source, and the other consists of IRs in a 2D square region from an array of 32 sources. This dataset is suitable for evaluating sound field analysis and synthesis methods. See the link below for the details: https://sh01k.github.io/MeshRIR/
DOI: 10.57754/fdat.pgnw8-kh792
title: Uppsala Corpus

description: The Uppsala Corpus of modern Russian texts was developed at the Department of Slavic Studies at Uppsala University, Sweden, under the direction of Lennart Lönngren, from whom we obtained the permission to use the Uppsala corpus for the SFB 441 project B01. All rights regarding the Uppsala corpus belong to the author. Corpus data may be used for research purposes only; commercial use of the corpus is prohibited. This corpus (Upsal'skij korpus russkix tekstov) consists of some 600 Russian texts with a total of one million running words (word tokens), equally divided between informative and literary prose. The informative texts are from between 1985 and 1989, while the literary texts, whose vocabulary does not date as quickly, cover a longer period, 1960-88. The corpus does not include poetry or drama.
description: Das Uppsala-Korpus moderner russischer Texte wurde am Slavischen Institut der Universität Uppsala unter Leitung von Lennart Lönngren erstellt. Die Rechte am Korpus liegen beim Autor. Die Daten des Korpus dürfen nur für wissenschaftliche Zwecke verwendet werden; eine kommerzielle Verwendung ist nicht erlaubt.
description: Research carried out in work package B01 of the SFB 441.
DOI: 10.5281/zenodo.10847727
title: Supplementary Data for ZeroPM D2.3 - Lessons learnt from previous applications of the essential-use concept

description: This dataset contains the data collected for all the studies which are mentioned in the ZeroPM deliverable D2.3 titled "Lessons learnt from previous applications of the essential-use concept".
DOI: 10.6084/m9.figshare.24786937
title: A Comparative Transcriptomic Study and Key Gene Targeting of Lamprey Gonadal Immune Response

description: The mammalian testis and ovary possess special immunocompetence, which is central to provide protection against pathogens. However, the innate immune responses to immune challenges in lamprey gonads are poorly understood. In this study, we extracted RNA from testis and ovary tissues of lampreys at 0 hour, 8 hours and 17 days after lipopolysaccharides (LPS) stimulation and performed transcriptome sequencing. While the transcriptome profiles of the two tissues were different for the most part, genes <i>LIP</i>, <i>LECT2</i>, <i>LAL2</i>, <i>GRN</i>, <i>ITLN</i>, and <i>C1q</i> were found to be the most significantly up-regulated genes in both. Quantitative Real-time PCR (qRT-PCR) analysis confirmed that these genes were upregulated after stimulation. Furthermore, immunohistochemical staining showed that these genes in lamprey gonads are expressed in high quantities and have a specific distribution. Taken together, our results suggest that these genes could play an essential role in response of the gonads to LPS induction. This research establishes a basis for investigating the immune mechanism of vertebrate gonads and presents a fresh concept for gaining insight into the evolutionary development of jawless vertebrates.
DOI: 10.6084/m9.figshare.24786937.v1
title: A Comparative Transcriptomic Study and Key Gene Targeting of Lamprey Gonadal Immune Response

description: The mammalian testis and ovary possess special immunocompetence, which is central to provide protection against pathogens. However, the innate immune responses to immune challenges in lamprey gonads are poorly understood. In this study, we extracted RNA from testis and ovary tissues of lampreys at 0 hour, 8 hours and 17 days after lipopolysaccharides (LPS) stimulation and performed transcriptome sequencing. While the transcriptome profiles of the two tissues were different for the most part, genes <i>LIP</i>, <i>LECT2</i>, <i>LAL2</i>, <i>GRN</i>, <i>ITLN</i>, and <i>C1q</i> were found to be the most significantly up-regulated genes in both. Quantitative Real-time PCR (qRT-PCR) analysis confirmed that these genes were upregulated after stimulation. Furthermore, immunohistochemical staining showed that these genes in lamprey gonads are expressed in high quantities and have a specific distribution. Taken together, our results suggest that these genes could play an essential role in response of the gonads to LPS induction. This research establishes a basis for investigating the immune mechanism of vertebrate gonads and presents a fresh concept for gaining insight into the evolutionary development of jawless vertebrates.
DOI: 10.6084/m9.figshare.25459573
title: USPTO-50K (raw)

description: Raw USPTO-50K data obtained from github.com/Hanjun-Dai/GLN.
DOI: 10.5519/qd.aqky64fa
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (5427421 records)
DOI: 10.5281/zenodo.10405670
title: Research on the impact of the digital economy on urban entrepreneurial activity: Evidence from China
DOI: 10.5281/zenodo.10405671
title: Research on the impact of the digital economy on urban entrepreneurial activity: Evidence from China