cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/x4cpyrd3rw.2
title: Original WB images lee et al Dev Cell

description: Immunoblotting data original scan.
DOI: 10.35115/37n9-5738
title: Synthetically Accessible Virtual Inventory (SAVI)
DOI: 10.17632/fkx39rvwgm.1
title: Grapefruit defect segmentation code

description: Source code of the Grapefruit Defect Segmentation Project.
DOI: 10.6084/m9.figshare.21763205
title: Knockdown of MsCSP17 and MsCSP18 disrupted the development of Mythimna separata larvae

description: Chemosensory proteins (CSPs) were necessary for insect sensory system to perform important processes such as feeding, mating, spawning, and avoiding natural enemies. However, their functions in non-olfactory organs have been poorly studied.
DOI: 10.6084/m9.figshare.21763205.v1
title: Knockdown of MsCSP17 and MsCSP18 disrupted the development of Mythimna separata larvae

description: Chemosensory proteins (CSPs) were necessary for insect sensory system to perform important processes such as feeding, mating, spawning, and avoiding natural enemies. However, their functions in non-olfactory organs have been poorly studied.
DOI: 10.21430/m300rmfhzq
title: Systems Biology Approach to Analysis of 2011-12 TIV Fluzone Influenza Vaccine Response in Healthy Individuals (see companion studies SDY74, SDY301)

description: This project will contribute to the overall vision and goals of this U19 by analyzing the immune response to Flu vaccination in healthy individuals. The knowledge generated in this Project will be transferred to Projects 3-5 where immune effects of vaccination will be studied in patients with underlying immune system alterations.
DOI: 10.21430/m30b3le7s5
title: Susceptibility and Resistance to Tuberculosis

description: To map, isolate and validate human genes that confer susceptibility or resistance to mycobacterium tuberculosis infections.
DOI: 10.21430/m36aep1t4u
title: Genotype Polymorphism effects on Typhoid Vaccine responses.

description: Innate immunity gene products are known to be the first-line of host-defense and to control and assist the adaptive immune system. This research represents the opportunity to study, in detail, the responses longitudinally to vaccination (using clinical examination and laboratory assays) in a large ethnic population. Consequently, a prioritized set of genes and gene products from both systems will be evaluated.
DOI: 10.21430/m381yqiyxy
title: Responses to and control of vaccinia in immunosuppressed Rhesus Macaques

description: To study the immune response to vaccinia vaccine in immunosuppressed Rhesus macaques and to develop a relevant preclinical model in which the efficacy and safety of vaccines in the setting of transplantation can be evaluated.
DOI: 10.21430/m389l5fqcn
title: Identify polymorphisms associated with risk for the development of myopericarditis following smallpox vaccine

description: The overall goal of this contract is to identify genetic differences that increase the risk for a major adverse event associated with smallpox vaccination - myocarditis - and to determine the mechanism by which these genetic differences confer risk.
DOI: 10.21430/m38y09r3r9
title: Efficacy and Safety Evaluation of Allergen Immunotherapy Co-Administered with Omalizumab (an anti-IgE Monoclonal Antibody) (ITN019AD)

description: A series of allergy shots may reduce symptoms of seasonal ragweed allergies. This study will determine whether taking a drug called omalizumab (also known as Xolair) before getting the allergy shots is more effective than allergy shots alone or other treatments, such as prescription antihistamines.
DOI: 10.21430/m398h5taxp
title: Risk Factors in Atopic Dermatitis for the Development of Eczema Herpeticum (ADEH 06)

description: Atopic Dermatitis (AD), also known as eczema, is a skin disease that causes the skin to be hot, dry and scaly, and have severe itching. There are different kinds of eczema. Eczema herpeticum (EH) is a type of eczema that spreads due to an underlying herpes virus infection. The purpose of this research study is to identify the risk factors that may cause EH.
DOI: 10.21430/m39s5fje1x
title: Immunity to Smallpox Vaccination

description: To map, isolate and validate human host genes that confer risk of either adverse reactions to smallpox vaccination or lack of specific immune response to the vaccine (specific anergy).
DOI: 10.21430/m3eer1f151
title: Antigenic and immunogenic properties of recombinant hemagglutinin proteins when produced in various protein expression systems

description: Assess the immunogenicity and antigenicity of recombinant hemagglutinin proteins produced and purified by various methods
DOI: 10.21430/m3fozz059r
title: Susceptibility and Resistance to Common Encapsulated Bacteria Infections

description: To map and isolate human host supergenes that confer general susceptibility and resistance to common encapsulated bacteria infections such as pneumococcus, meningococcus, and H. influenza.
DOI: 10.21430/m3gczx3f7x
title: ADVN Biomarker Registry Study: CMI-HSV Substudy

description: To evaluate AD subjects cell mediated immunity CMI responses to Herpes simplex virus.
DOI: 10.21430/m3h2i5wf3k
title: Susceptibility to Influenza Infection and Complications

description: To map, isolate and validate human genes that confer susceptibility or resistance to influenza infection and its complications.
DOI: 10.21430/m3h2lboi83
title: Assess the association between immune response genotype set and susceptibility to neuroinvasive disease.

description: The severity of West Nile virus (WNv) infections are a consequence of genetic factors that result in increased WNv replication and subsequent immune pathology in individuals with severe (encephalitis or meningitis) disease. Our preliminary draft of 156 genes includes potential candidates to study variation in the severity of WNv infections. Using a candidate gene approach we have also selected a panel of candidate immune response genes for SNP analysis guided by the collective understanding of virus-host interactions. This portion of the study will genotype these genes in a population of WNv patients and control s to identify genes associated with variations in the severity of WNv disease.
DOI: 10.21430/m3i44h8r17
title: Systems scale interactive exploration reveals quantitative and qualitative differences in response to 2009-2010 Fluzone influenza vaccine and pneumococcal vaccine

description: Systems approach to study immune response to seasonal influenza and 23-valent pneumoccocal vaccination in healthy adults.
DOI: 10.21430/m3k3uzwk6s
title: Humoral and Cell-Mediated Immune Responses to Vaccinia Virus Immunization

description: Our broad objective is to examine the role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia vaccine-induced humoral and cell-mediated immune responses among a cohort of 1,000 vaccinated subjects.
DOI: 10.21430/m3l5go913j
title: Genotyping and gene function in healthy volunteers

description: Functionally important genetic variants (certain naturally occurring SNPs) in a set of candidate genes that are known to be relevant for immune response to protein antigens, are expected to be associated with AbPA response and B-cell physiology.
DOI: 10.21430/m3op2qb064
title: ADVN Biomarker Registry: Neutrophil Substudy

description: To evaluate the chemotactic function of peripheral blood leukocytes such as neutrophils in subjects with ADEH+, ADEH-, and NA controls.
DOI: 10.21430/m3p9t1ufb9
title: CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses

description: Flow cytometry was used to measure the expression of IL2, IFN gamma and TNF alpha in CD8+ peripheral blood mononuclear cells isolated from healthy naive donors following stimulation with whole virus.
DOI: 10.21430/m3stai2v6t
title: Cellular and molecular characterization of the immune response in healthy NIH employees at baseline and after immunization with the H1N1 or seasonal influenza vaccines

description: The Center for Human Immunology, Autoimmunity, and Inflammatory Diseases proposes this protocol designed to obtain blood from healthy adult subjects (NIH employees) prior to vaccination and then at various time points after receiving the FDA-licensed seasonal and H1N1 influenza vaccine. These samples will be used to perform a comprehensive and detailed analysis of the immune system at baseline and in response to vaccination. To our knowledge, this protocol will be the first study to characterize the human cellular and molecular immune system parameters, or immunome, in a large number of healthy adults (NIH employees). This information may be useful in designing newer, more effective vaccines to prevent the spread of H1N1 influenza.
DOI: 10.21430/m3thufgism
title: Comprehensive study of germinal center development and antibody response

description: To comprehensively study the humoral response to immunization with rPA with or without Alum using in vitro antibody analyses and tissue staining.
DOI: 10.21430/m3tt5spdgv
title: Genetic Associations in subjects of anthrax trial [Anthrax Vaccine Adsorbed: Human Reactogenicity and Immunogenicity Trial to Address Change in Route of Administration and Dose Reduction (AVA000)]

description: Genetic Associations in the AVA000 trial population and functional mechanism of that variation. Keywords in the study are: SNPs, Anthrax Vaccine Adsorbed (AVA), Illumina BeadChip array, Pyrosequencing, Genotyping, Genetic variation, Saline placebo
DOI: 10.21430/m3vdxbdprq
title: Determine of anti-PA titer kinetics

description: Determine of anti-PA titer kinetics
DOI: 10.21430/m3w4xgm811
title: Immune response of patients during pregnancy

description: Immunological changes play a physiological role in pregnancy, however they may also have consequences for vaccine response and immunotherapy, which ultimately could impact maternal and fetal survival in the event of transmission of infectious diseases and/or bioterrorism.<br><br>The purpose of this study is to characterize the changes in human immune response that occurs during pregnancy and lactation.
DOI: 10.21430/m3wc8vq7oi
title: CD4+ T Cells Generated from Encounter with Seasonal Influenza Viruses and Vaccines Can Recognize Epitopes Derived from Pandemic H1N1 Virus

description: ELISPOT was used to enumerate the number of IFN gamma-secreting CD4+ peripheral blood mononuclear cells isolated from healthy naive donors following stimulation with APCs treated with peptide pools or whole virus
DOI: 10.21430/m30usx09nf
title: Exploration of molecular mechanism(s) for TLR ligand response differences between adults and neonates

description: The study will perform expression microarrays on RNA from purified subsets of neonatal and adult APCs either stimulated or not stimulated with TLR ligands of interest. Arrays will be performed on cells from neonatal and adult individuals and the arrays for all will be run in parallel on the same day.
DOI: 10.21430/m30zc7u5iw
title: Human TLR4/MD-2 knockin (KI) mice and comparison of TLR4 agonists ability to enhance innate immunity and development of adaptive immunity in mice

description: The study's aim is to generate human TLR and MD-2 knockin (KI) mice to assess host responses to infections with Y. pestis and to evaluate the safety and efficacy of purified/synthetic TLR4 ligand immunotherapeutics.
DOI: 10.21430/m30zxxdzlp
title: Comparative Pathology in Ferrets Infected with H1N1 Influenza A Viruses Isolated from Different Hosts

description: Virus replication and pulmonary disease pathogenesis in ferrets following intranasal infection with H1N1
DOI: 10.21430/m320h8xyfg
title: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2011 (See companion studies SDY311 2010 / SDY312 2009 / SDY314 2008 / SDY315 2012)

description: Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
DOI: 10.21430/m321yj41hj
title: Role for CaMKIV in the Regulation of Antibody Responses to Influenza Vaccine (See companion studies SDY61 2007 / SDY269 2008 / SDY270 2009)

description: To demonstrate that the gene signatures identified in this study can generate new hypotheses, we selected one gene in the predictive signature, calcium/calmodulin-dependent protein kinase IV (CaMKIV), for functional validation experiments. The CamkIV gene is involved in several processes of the immune system, such as T cell development,inflammatory response and hematopoietic stem cell maintenance. However, nothing is known about the possible role of CaMKIV in B cell responses. The fold-change in expression of the CaMKIV gene on day 3 post-TIV vaccination is negatively correlated with the antibody response on day 28 post-vaccination in two independent trials. Additionally, fold-change expression of CaMKIV is negatively correlated with the expansion of IgG-secreting plasmablasts at day 7, suggesting a possible role for this gene in the regulation of antibody responses to influenza vaccination.
DOI: 10.21430/m33c1gncxf
title: Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes

description: High dimensional analysis of CD8+ T cell phenotype and function
DOI: 10.21430/m33mg60qvq
title: Live Kidney Donor Study -Renal Function Study (RELIVE-06)

description: As part of a cross-sectional study of living kidney donors from 5 to more than 50 years following donor nephrectomy, this study's goal is to accurately measure current Glomerular Filtration Rate (GFR), then to look at the change in GFR from pre to post donation and from early to late time points after donation. This study builds on information generated from RELIVE-01 and RELIVE-04. Through direct contact with previous living kidney donors, this study aims to quantify changes in renal function (measured GFR) from pre to early post donation using data already collected in a related retrospective study (RELIVE 01), and the change in renal function from early (in the first 2 years) post donation to late (beyond the first 2 years) post donation by repeating a GFR measurement. Cross-sectional GFR measurements from black donors compared to white donors matched for age, sex, weight and time from donation will provide data on differences in GFR late after donor nephrectomy by race. When compared to GFR estimates using predictive equations at each of these time points, we can evaluate the accuracy of estimated GFR in white and black donor populations.
DOI: 10.21430/m33msdrj55
title: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2010 (See companion studies SDY315 2012 / SDY312 2009 / SDY314 2008 / SDY112 2011)

description: Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
DOI: 10.21430/m3495bhp30
title: Live Lung Donor Retrospective Study (RELIVE-02)

description: The purpose of this study is to determine the mortality, the early postoperative morbidity, and the occurrence of end stage lung disease for participants who underwent donor lobectomy between 1993 and 2006. Participants in this study have had donor lobectomy at the University of Southern California in Los Angeles or the Washington University Medical Center and Barnes-Jewish Hospital in St. Louis.
DOI: 10.21430/m34x4ulp41
title: A Biomarker-based Pilot Study of Cockroach Sublingual Immunotherapy in Cockroach Sensitive Adults With Asthma and/or Perennial Allergic Rhinitis (BioCSI/ICAC-12)

description: There is currently no effective way to prevent development of allergic rhinitis (nasal allergies) and asthma and no cure. Sublingual immunotherapy (SLIT), a type of therapy in which allergens are placed under the tongue, may be a way to control and possibly prevent allergic rhinitis and asthma. However, detailed research of this approach is limited. The purpose of this study is to evaluate the safety and efficacy of a sublingual cockroach extract given to adults with perennial allergic rhinitis, asthma, or both.
DOI: 10.21430/m35hmsdthh
title: Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2014/2015 Cohort (see companion studies SDY369, SDY376, SDY372)

description: Transcriptional profiling of peripheral blood of pediatric patients with juvenile dermatomyositis (JDM) before/after vaccination with seasonal influenza vaccine (Fluzone)
DOI: 10.21430/m35j0wa7cr
title: Responses to Influenza Vaccination in Systemic Lupus Year 6 2010-2011

description: Compare the major components of the normal immune response to flu vaccination in SLE patients and control subjects in order to identify abnormalities in SLE group of immunocompromised individuals.
DOI: 10.21430/m35opgluth
title: B and T Cell Determinants of Influenza Vaccine Responses in the Elderly 2009 (see companion study SDY460)

description: Application of high-throughput DNA sequencing of the IGH gene rearrangements to study the B cell repertoires over two successive years in 27 individuals ranging in age from 20 to 89 years old.
DOI: 10.21430/m35ynxhf8k
title: Orchestration of CD4 T cell epitope preferences after multi-peptide immunization

description: A detailed understanding of the molecular and cellular mechanisms that underlie epitope preferences in T cell priming is important for vaccines designed to elicit a broad T cell response. Protein vaccinations generally elicit CD4 T cell responses that are skewed toward a small fraction of epitopes, a phenomenon known as immunodominance. This characteristic of T cell responses, that limits the diversity of CD4 T cell recognition, is generally attributed to intracellular antigen processing. However, we recently discovered that immunodominance hierarchies persist even after vaccination with synthetic peptides. In this study, we probed the regulatory mechanisms that cause diminished CD4 T cell responses to subdominant peptides after such multi-peptide immunization in mice. We have found that the delivery of subdominant and dominant epitopes on separate dendritic cells rescues expansion of less favored CD4 T cells. Furthermore, through the use of genetic models and inhibitors, we have found that selective losses in CD4 T cell responses are mediated by an IFN-gamma-induced pathway, involving indoleamine 2,3-dioxygenase (IDO), and that regulatory T cell (Treg) activities may also regulate preferences in CD4 T cell specificity. We propose that after multi-peptide immunization, the expansion and differentiation of dominant T cells initiate complex regulatory events that determine the final peptide specificity of the elicited CD4 T cell response.
DOI: 10.21430/m36i96jiiz
title: The Role of Peroxisome Proliferator-Activated Receptor gamma in Immune Responses to Enteroaggregative Escherichia coli Infection

description: Enteroaggregative Escherichia coli (EAEC) is recognized as an emerging cause of persistent diarrhea and enteric disease worldwide. Mucosal immunity towards EAEC infections is incompletely understood;due in part to the lack of appropriate animal models. This study presents a new mouse model and investigates the role of peroxisome proliferator-activated receptor gamma (PPARg) in the modulation of host responses to EAEC in nourished and malnourished mice. Methods/Principal Findings: Wild-type and T cell-specific PPARg null C57BL/6 mice were fed protein-deficient diets at weaning and challenged with 5x10^9 cfu EAEC strain JM221 to measure colonic gene expression and immune responses to EAEC. Antigen-specific responses to E. coli antigens were measured in nourished and malnourished mice following infection and demonstrated the immunosuppressive effects of malnutrition at the cellular level. At the molecular level, both pharmacological blockade and deletion of PPARg in T cells resulted in upregulation of Tgfb1, IL-6, IL-17 and anti-microbial peptides, enhanced Th17 responses, fewer colonic lesions, faster clearance of EAEC, and improved recovery. The beneficial effects of PPAR gamma blockade on weight loss and EAEC clearance were abrogated by neutralizing IL-17 in vivo. Conclusions: Our studies provide in vivo evidence in support of supporting the beneficial role of mucosal innate and effector T cell responses on the EAEC burden and suggest pharmacological blockade of PPARg as a novel therapeutic intervention for EAEC infection.
DOI: 10.21430/m36nxb3cp1
title: Gene expression changes in neonatal and adult plasmacytoid dendritic cells (pDC) and myeloid dendritic cells (mDC) upon TLR7/8 stimulation

description: Differences in gene expression changes between neonatal and adult plasmacytoid dendritic cells upon stimulation with TLR7/8 may point to specific signaling pathways which, in turn, may account for the known differential immune response of babies to vaccination or infection when compared to adults.
DOI: 10.21430/m3736k8gmy
title: Human Aging and CMV

description: Delineate the effect of aging from that of cytomegalovirus (CMV) infection upon relative and absolute changes in blood T cell subsets.
DOI: 10.21430/m37ancfrhf
title: Predictive computational modeling of the mucosal immune responses during Helicobacter pylori infection

description: We developed a validated mathematical and computation model of immune responses to H. pylori infection that delineate how immune responses to H. pylori correlate with initiation, progression and outcome of disease.
DOI: 10.21430/m37ixt07lu
title: Determining the effect of different adjuvants and doses on the immune response

description: Inbred C57BL/6 female mice (8-10 weeks old) were immunized subcutaneously according to the 8 immunization groups. Serum samples were collected weekly from 2 staggered groups and analyzed for PA-specific (Total Ig, IgG1, IgG2a, and IgG3) and neutralizing antibody titers. Also, spleen, thymus, and brachial and inguinal lymph nodes will be harvested from 1 of each group at 4 time points for histology.
DOI: 10.21430/m37kmo7jlw
title: Immune Responses to Seasonal TIV 2010-2011 Influenza Vaccination in Humans (see companion study SDY396,SDY564)

description: To use system biology approaches to compare differences in immune responses to vaccine
DOI: 10.21430/m37ngthmds
title: Apoptosis and other immune biomarkers predict influenza vaccine (TIV 2008) responsiveness

description: In an effort to indentify benchmarks of immunological health, influenza vaccination was used in 30 young (20 to 30 years) and 59 older subjects (60 to 89 years) as models for strong and weak immune responses, respectively.
DOI: 10.21430/m38jpmtp8s
title: TIV 2008 vaccination of CD95-/- mice and ELISA for detection of influenza-specific antibodies

description: Evaluation of influenza vaccination immune response in mice
DOI: 10.21430/m38lff1qaa
title: Live Lung Donor Cross-sectional Cohort Study (RELIVE-05)

description: The Live Lung Donor Cross-sectional Cohort Study (RELIVE-05) aimed to determine the effects of live lung donation on lung function, quality of life, morbidity, psychosocial status, satisfaction with live lung donation, and decision-making associated with live lung donation. This study built on the separate but related RELIVE-02 retrospective study that assessed the effects of live lung donation on survival and short-term morbidity. This multicenter cross-sectional cohort study invited the RELIVE-02 living former live lung donors to undergo questionnaire and pulmonary function test assessments to further determine the long-term outcomes of live lung donation. This study used preoperative donor pulmonary function data and normative data to assist with assessing the effects of live lung donation on lung donors.