DOI: 10.5061/dryad.wdbrv160w
title: Hoi1 targets the yeast BLTP2 protein to ER-PM contact sites to regulate lipid homeostasis

description: Membrane contact sites between organelles are important for maintaining
 cellular lipid homeostasis. Members of the recently identified family of
 bridge-like lipid transfer proteins (BLTPs) span opposing membranes at
 these contact sites to enable the rapid transfer of bulk lipids between
 organelles. While the VPS13 and ATG2 family members use organelle-specific
 adaptors for membrane targeting, the mechanisms that regulate other
 bridge-like transporters remain unknown. Here, we identify the conserved
 protein Ybl086c, which we name Hoi1 (Hob interactor 1), as an adaptor that
 targets the yeast BLTP2-like proteins Fmp27/Hob1 and Hob2 to ER-PM contact
 sites. Two separate Hoi1 domains interface with alpha-helical projections
 that decorate the central hydrophobic channel on Fmp27, and loss of these
 interactions disrupts cellular sterol homeostasis. The mutant phenotypes
 of BLTP2 and HOI1 orthologs indicate these proteins act in a shared
 pathway in worms and flies. Together, this suggests that Hoi1-mediated
 recruitment of BLTP2-like proteins represents an evolutionarily conserved
 mechanism for regulating lipid transport at membrane contact sites.
description: # Hoi1 targets the yeast BLTP2 protein to ER-PM contact sites to regulate
 lipid homeostasis Dataset DOI:
 [10.5061/dryad.wdbrv160w](10.5061/dryad.wdbrv160w) ## Description of the
 data and file structure This file contains the raw data used to generate
 the figures produced by the Bashirullah Lab in the preprint: Dziurdzik SK,
 Sridhar V, Eng H, Neuman SD, Yan J, Davey M, Taubert S, Bashirullah A,
 Conibear E. Hoi1 targets the yeast BLTP2 protein to ER-PM contact sites to
 regulate lipid homeostasis. bioRxiv [Preprint]. 2025 Feb
 19:2025.02.11.637747. doi: 10.1101/2025.02.11.637747. PMID: 39990326;
 PMCID: PMC11844476. This manuscript characterizes the function of a novel
 adapter protein that regulates localization of the bridge-like lipid
 transfer protein (BLTP) *BLTP2* to endoplasmic reticulum-plasma membrane
 (ER-PM) contact sites. This dataset includes quantitative reverse
 transcription PCR (qRT-PCR) data and body size/pupa volume data. Each file
 name contains a reference to the figure where the data is published, as
 well as a description of the type of data included in the file. ### Files
 and variables #### Figure7FqRTPCRData.csv This file contains the raw cycle
 threshold (Ct) values generated via quantitative reverse transcription
 polymerase chain reaction (qRT-PCR) to validate RNAi knockdown efficiency
 for *hobbit-RNAi* and *CG8671-RNAi *in *Drosophila* whole animals (WA) at
 0 h after puparium formation (PF) using the ubiquitous *act-GAL4* driver.
 Note that “&gt;” in genotypes is shorthand for *GAL4, UAS* (*e.g.,
 act&gt;hob(i)* is short for *act-GAL4 UAS-hob(i)*). Number of variables: 4
 Number of header rows: 1 Number of rows: 6 Variable list: Genotype
 (alphanumeric): the genotype and replicate number analyzed *rp49* Ct
 (numeric): cycle threshold (Ct) value for the reference gene *rp49* *hob*
 Ct (numeric): cycle threshold (Ct) value for *hob* *CG8671* Ct (numeric):
 cycle threshold (Ct) value for *CG8671* Data type: alphanumeric, numeric
 #### Figure7HPupaVolumeData.csv This file contains the raw data used to
 calculate body size/pupa volume in control (*act&gt;/+*), ubiquitous
 *hob-RNAi* knockdown (*act&gt;hob(i)*), and ubiquitous *CG8671-RNAi*
 knockdown (*act&gt;CG8671(i)*). The length and width of each pupa (in mm)
 were measured in Adobe Photoshop CS6, and pupa volume was calculated using
 a published formula. Note that “&gt;” in genotypes is shorthand for *GAL4,
 UAS* (*e.g., act&gt;hob(i)* is short for *act-GAL4 UAS-hob(i)*). Number of
 variables: 3 Number of header rows: 2 Number of rows: 100 Variable list:
 Genotype (alphanumeric): the genotype being analyzed pupa width (mm)
 (numeric): the measured width of each pupa pupa length (mm) (numeric): the
 measured length of each pupa Data type: alphanumeric, numeric ##
 Code/software Data are provided as .csv files, which can be opened by any
 freely available word processing software.