DOI: 10.5061/dryad.dbrv15f9r
title: Conidia productivity and whitefly insecticidal activity of Beauveria bassiana JEF-507

description: Silverleaf whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a
 destructive insect pest damaging to diverse crops by vectoring several
 plant pathogenic viruses, which consequently causes economic losses in
 crop production. As the resistance of whiteflies to chemical insecticides
 is increasing, this study aims to investigate the potential of
 entomopathogenic fungi as an alternative. A total of 72 entomopathogenic
 fungal isolates, collected from soils using Tenebrio molitor larvae as an
 insect baiting method, were assessed for their virulence against 2nd
 nymphs of whitefly. Their virulence was assayed by dipping
 whitefly-infested tomato leaves in fungal conidia suspensions at 1.0 × 107
 conidia/ml. Among the tested isolates, two isolates of Beauveria bassiana
 JEF-462 and JEF-507 showed high virulence. In the assessment of virulence
 depending on conidia concentrations, the estimated LC50 values for JEF-462
 and JEF-507 were similarly 8.7~14.0 × 107 conidia/ml. However, B. bassiana
 JEF-507 showed higher conidial productivity and
 thermotolerance on most of tested 12 grain substrates than B.
 bassiana JEF-462, and millet was the most suitable grain substrate.
 Additionally, siloxane as a surfactant was able to sufficiently exhibit
 the insecticidal activity of JEF-507 against whitefly nymphs compared to
 other surfactants. In a pot-based greenhouse trial, JEF-507 showed higher
 control efficacy than chemical insecticides, dinotefuran and spinetoram.
 This work suggests that B. bassiana JEF-507 could be competitively used as
 a biopesticide to control silverleaf whiteflies whilst overcoming current
 resistance issues. The JEF-507 isolate has been registered in Korea, 2022
 and successfully commercialized as the name of Chongchae-Stop® in this
 local market to control whitefly and thrips.
description: GENERAL INFORMATION 1. Title of Dataset: Data from: Conidia productivity
 and whitefly insecticidal activity of Beauveria bassiana JEF-507 2. Author
 Information Corresponding Investigator Name: Prof. Jae Su Kim Institution:
 Jeonbuk national university, Jeonju, Korea Email:
 [jskim10@jbnu.ac.kr](mailto:jskim10@jbnu.ac.kr) Co-investigator 1 Name: Dr
 Jong-Cheol Kim Institution: Jeonbuk national university, Jeonju, Korea
 Email: [jc.kim124@gmail.com](mailto:jc.kim124@gmail.com) Co-investigator 2
 Name: Sehyeon Paek Institution: Jeonbuk national university, Jeonju, Korea
 Email: [yyy5157@naver.com](mailto:yyy5157@naver.com) 3. Date of data
 collection: 2019-2020 4. Geographic location of data collection: Jeonju in
 Korea 5. Funding sources that supported the collection of the data:
 Ministry of Agriculture, Food and Rural Affairs (Korea) DATA & FILE
 OVERVIEW 1. File List: File 1 Name: 1.Fungal virulence screening.xlsx File
 2 Name: 3a.Quantitative virulence of B. bassiana isolates.xlsx File 3
 Name: 3b.Thermotolerance of B. bassiana isolates.xlsx File 4 Name:
 4.Dose-dependent insecticidal activity of B. bassiana JEF-462 and
 JEF-507.xlsx File 5 Name: 5.Conidial productivity on industrial grain
 substrates.xlsx File 6 Name: 6.Thermotolerance of conidia on grain
 substrates.xlsx File 7 Name: 7.Insecticidal efficacy of surfactants.xlsx
 File 8 Name: 8.Insecticidal activity of B. bassiana JEF-462 and JEF-507 on
 pot condition.xlsx DATA-SPECIFIC INFORMATION FOR: 1.Fungal virulence
 screening.xlsx 1. Number of variables: 5 2. Number of cases/rows: 74 3.
 Variable List: Treatment: control, ERL836, JEF-003, JEF-006, JEF-013,
 JEF-014, JEF-016, JEF-023, JEF-026, JEF-029, JEF-038, JEF-045, JEF-055,
 JEF-061, JEF-091, JEF-096, JEF-104, JEF-105, JEF-125, JEF-133, JEF-137,
 JEF-139, JEF-140, JEF-141, JEF-142, JEF-145, JEF-146, JEF-147, JEF-152,
 JEF-159, JEF-161, JEF-163, JEF-169, JEF-174, JEF-182, JEF-186, JEF-188,
 JEF-189, JEF-193, JEF-198, JEF-201, JEF-232, JEF-239, JEF-241, JEF-245,
 JEF-256, JEF-259, JEF-261, JEF-262, JEF-264, JEF-270, JEF-279, JEF-299,
 JEF-341, JEF-347, JEF-350, JEF-355, JEF-362, JEF-367, JEF-382, JEF-383,
 JEF-388, JEF-389, JEF-396, JEF-397, JEF-399, JEF-401, JEF-422, JEF-462,
 JEF-479, JEF-501, JEF-507, JEF-509 DBT: number of survival whitefly Before
 Treatment; 0-100 Day 3: number of survival whitefly at Day 3 After
 Treatment; 0-100 Day 4: number of survival whitefly at Day 4 After
 Treatment; 0-100 Day 5: number of survival whitefly at Day 5 After
 Treatment; 0-100 4. Missing data codes: None 5. Abbreviations used: DBT;
 Day Before Treatment, Day 3; Day 3 After Treatment, Day 4; Day 4 After
 Treatment, Day 5; Day 5 After Treatment 6. Other relevant information: To
 select highly virulent isolates against whiteflies among various
 entomopathogenic fungi, tomato leaves with whitefly nymphs were treated
 with conidia suspensions (1.0 ×10^7 conidia/ml) of each isolates using the
 dipping method to assess for insecticidal activity. DATA-SPECIFIC
 INFORMATION FOR: 3a.Quantitative virulence of B. bassiana isolates.xlsx 1.
 Number of variables: 6 2. Number of cases/rows: 34 3. Variable List:
 Treatment: N.C., ERL836, JEF-013, JEF-163, JEF-341, JEF-347, JEF-350,
 JEF-422, JEF-462, JEF-501, JEF-507 Replicate: 3 DBT: number of survival
 whitefly Before Treatment; 0-100 Day 3: number of survival whitefly at Day
 3 After Treatment; 0-100 Day 4: number of survival whitefly at Day 4 After
 Treatment; 0-100 Day 5: number of survival whitefly at Day 5 After
 Treatment; 0-100 4. Missing data codes: None 5. Abbreviations used: N.C.;
 Non-treated control, DBT; Day Before Treatment, Day 3; Day 3 After
 Treatment, Day 4; Day 4 After Treatment, Day 5; Day 5 After Treatment 6.
 Other relevant information: To select highly virulent isolates against
 whiteflies among various Beauveria bassiana, tomato leaves with whitefly
 nymphs were treated with conidia suspensions (1.0 ×10^7 conidia/ml) of
 each isolates using the dipping method to assess for insecticidal
 activity. DATA-SPECIFIC INFORMATION FOR: 3b.Thermotolerance of B. bassiana
 isolates.xlsx 1. Number of variables: 4 2. Number of cases/rows: 151 3.
 Variable List: Treatment: ERL836, JEF-013, JEF-163, JEF-341, JEF-347,
 JEF-350, JEF-422, JEF-462, JEF-501, JEF-507 Heat: Heat treatment time;
 0-120 min Rep: Replicate; 3 Germination rate: Germination rate of conidia
 after 18 hours of incubation following heat treatment; 0-100 4. Missing
 data codes: None 5. Abbreviations used: none 6. Other relevant
 information: The conidia suspension of Beauveria bassiana was treated at
 45 degrees for 0, 30, 60, 90, and 120 minutes, and the germination rate of
 the conidia was examined to identify strains with high heat stability.
 DATA-SPECIFIC INFORMATION FOR: 4.Dose-dependent insecticidal activity of
 B. bassiana JEF-462 and JEF-507.xlsx 1. Number of variables: 4 2. Number
 of cases/rows: 145 3. Variable List: Treatment: N.C., Dinotefuran, Bb
 JEF-462_5, Bb JEF-462_6, Bb JEF-462_7, JEF-507_5, JEF-507_6, JEF-507_7
 Day: Day after treatment; 0-5 Rep: Replicate; 3 Mortality: Mortality of
 whitefly nymphs; 0-100 4. Missing data codes: None 5. Abbreviations used:
 N.C.; Non-treated control, Bb JEF-462_5; Beauveria bassiana JEF-462
 (1.0*10^5 conidia/ml) treatment, Bb JEF-462_6; Beauveria bassiana JEF-462
 (1.0*10^6 conidia/ml) treatment, Bb JEF-462_7; Beauveria bassiana JEF-462
 (1.0*10^7 conidia/ml) treatment, JEF-507_5; Beauveria bassiana JEF-507
 (1.0*10^5 conidia/ml) treatment, JEF-507_6; Beauveria bassiana JEF-507
 (1.0*10^6 conidia/ml) treatment, JEF-507_7; Beauveria bassiana JEF-507
 (1.0*10^7 conidia/ml) treatment 6. Other relevant information: The two
 isolates (Beauveria bassiana JEF-462 and JEF-507) were treated at
 different concentrations (1.0*10^5,6,7 conidia/ml) on whitefly nymphs on
 tomato leaves using the dipping method to evaluate their insecticidal
 activity. DATA-SPECIFIC INFORMATION FOR: 5.Conidial productivity on
 industrial grain substrates.xlsx 1. Number of variables: 7 2. Number of
 cases/rows: 73 3. Variable List: Grain: Cassia seed, Millet, Oatmeal, Mung
 beans, Perilla seed, Buckwheat, Barley, Sorghum, Rice, Italian millet,
 Sesame, Brown rice Isolate: Bb JEF-462 (Beauveria bassiana JEF-462), Bb
 JEF-507 Rep: Replicate; 3 Conida: Number of conidia (x10^7 /g) 4. Missing
 data codes: None 5. Abbreviations used: Conida: Number of conidia (x10^7
 /g) 6. Other relevant information: The two isolates (Beauveria bassiana
 JEF-462 and JEF-507) were cultured on 12 different grain substrates to
 determine conidia productivity. DATA-SPECIFIC INFORMATION FOR:
 6.Thermotolerance of conidia on grain substrates.xlsx 1. Number of
 variables: 5 2. Number of cases/rows: 361 3. Variable List: Grain: Cassia
 seed, Millet, Oatmeal, Mung beans, Perilla seed, Buckwheat, Barley,
 Sorghum, Rice, Italian millet, Sesame, Brown rice Isolate: Bb JEF-462, Bb
 JEF-507 Heat: Heat treatment time; 0-120 min Rep: Replicate; 3 Germination
 rate: Germination rate of conidia after 18 hours of incubation following
 heat treatment; 0-100 4. Missing data codes: None 5. Abbreviations used:
 Bb; Beauveria bassiana 6. Other relevant information: The conidia obtained
 by culturing the two isolates (Beauveria bassiana JEF-462 and JEF-507) on
 12 different grain substrates were heat-treated at 45 degrees for 0-120
 minutes, then incubated for 18 hours to determine the germination rate of
 the conidia and evaluate heat stability. DATA-SPECIFIC INFORMATION FOR:
 7.Insecticidal efficacy of surfactants.xlsx 1. Number of variables: 4 2.
 Number of cases/rows: 55 3. Variable List: Surfactant: S-1, S-3, LE-1,
 LE-3, CO-1, CO-2 Treatment: N.C., Bb JEF-462, Bb JEF-507 Rep: Replicate; 3
 Mortality: Mortality of whitefly nymphs; 0-100 4. Missing data codes: None
 5. Abbreviations used: N.C.; Non-treated Control S-1; 0.01% siloxane, S-3;
 0.03% siloxane, LE-1; 0.01% polyoxyethylene-7-lauryl ether, LE-3; 0.03%
 polyoxyethylene-7-lauryl ether, CO-1; 0.01% polyoxyethylene-12-castorethe,
 CO-3; 0.03% polyoxyethylene-12-castorethe 6. Other relevant information:
 Three surfactants were diluted at two different concentrations, and the
 conidia suspensions (1.0*10^7 conidia/ml) of the two isolates (Beauveria
 bassiana JEF-462 and JEF-507) were prepared with each surfactant to
 evaluate insecticidal activity against whitefly nymphs. DATA-SPECIFIC
 INFORMATION FOR: 8.Insecticidal activity of B. bassiana JEF-462 and
 JEF-507 on pot condition.xlsx 1. Number of variables: 4 2. Number of
 cases/rows: 76 3. Variable List: Treatment: N.C., Bb JEF-462, Bb JEF-507
 Day: Day after treatment; 0-8 Rep: Replicate; 3 Mortality: Mortality of
 whitefly nymphs; 0-100 4. Missing data codes: None 5. Abbreviations used:
 N.C.; Non-treated Control, Bb JEF-462; Beauveria bassiana JEF-462, Bb
 JEF-507; Beauveria bassiana JEF-507 6. Other relevant information: The
 conidia suspensions of the two isolates (Beauveria bassiana JEF-462 and
 JEF-507) were prepared to evaluate insecticidal activity in comparison
 with chemical insecticides. The conidia suspensions were prepared at a
 concentration of 1.0*10^7 conidia/ml, and the chemical insecticide was
 diluted to the recommended concentration. The solutions were then sprayed
 on tomato plants with whitefly nymphs to assess insecticidal activity.