DOI: 10.5061/dryad.9p8cz8wvd
title: Reproductive systems of Hohenbergia Schult. & Schult.f. (Bromelioideae: Bromeliaceae) endemic to the Atlantic Forest

description: The intense fragmentation and anthropogenic pressure affecting the
 Atlantic Forest highlight the need for species conservation,
 particularly those in the genus Hohenbergia. The H. stellata complex
 includes endemic species that rely on diverse reproductive
 strategies, which are essential for their maintenance
 and diversification. Research on the reproductive systems of
 these species is crucial for understanding their adaptability and
 ensuring their preservation. This study aimed to investigate the
 reproductive systems of species within the Hohenbergia stellata
 complex, analyzing the potential occurrence of reproductive
 barriers and their reliance on cross-pollination. The
 pollen-to-ovule ratio was assessed, along with various
 methods for evaluating reproductive systems in H. amargosensis,
 H. belemii, H. capitata, H. ituberaensis, H. nidularioides, H. rosea, and
 H. stellata. Hohenbergia capitata and H. nidularioides are partially
 self-compatible species, while the others are self-incompatible. All of
 them reproduce through cross-pollination, with a greater number of seeds
 produced by natural pollination. The data obtained on the reproductive
 aspects of Hohenbergia species are important to support studies aimed at
 the improvement and conservation of these species.
description: # Reproductive systems of *Hohenbergia* Schult. & Schult.f.
 (Bromelioideae: Bromeliaceae) endemic to the Atlantic Forest Dryad DOI:
 https://doi.org/10.5061/dryad.9p8cz8wvd This dataset provides detailed
 information about the reproductive systems of *Hohenbergia*, a genus
 belonging to the family Bromeliaceae. Data were collected between 2022 and
 2023 and include assessments of pollen grain germination, pollen tube
 growth, fruit production, number of seeds per fruit, number of ovules per
 flower, and number of pollen grains produced per flower. The dataset aims
 to provide insights into the reproductive biology of the species,
 contributing to studies in ecology, evolution, and conservation. ## Data
 Description and File Structure The data are organized into five files:
 **2022_2023_Reproductive_systems_of_Hohenbergia_Evaluation_of_pollen_grain_germination_and_pollen_tube_growth_within_the_pistil.txt:** Contains data on pollen grain germination and pollen tube growth in Hohenbergia flowers. The data are organized in a tabular format and include columns with species name, treatment, number of replicates, and qualitative values for each variable studied. Species: Plant species. Pollination_type: Type of pollination (e.g., Natural, Spontaneous, Artificial). Replicate: Experimental replicate number. Abbreviations: PGSS = pollen grains adhering to the surface of the stigma; PGGS = pollen grains germinating on the surface of the stigma were evalu ated according to the scale: 0) none; 1) 1–25 pollen grains; 2) 26–50 pollen grains; 3) more than 50 pollen grains. PTGP = pollen tube growth on the pistil was evaluated according to the scale: 0) pollen grains not germinating on the surface of the stigma; 1) pollen grains germinating on the surface of the stigma; 2) pollen tubes arrested in the first third of the style; 3) pollen tubes arrested in the second third of the style; 4) pollen tubes arrested in the final third of the style; 5) pollen tubes in the region of the ovary; 6) pollen tube close to the ovule; 7) pollen tube penetrating the micropyle. OPTM = ovules penetrated by a pollen tube into their micropyle: 0) none; 1) 1–5 ovules; 2) 5–10 ovules; 3) &gt; 10 ovules. **2022_2023_Reproductive_systems_of_Hohenbergia_Fruits_with_Seeds.txt:** Records the number of fruits formed with seeds. Data are organized in a tabular format with columns: Species: Plant species. Treatment: Pollination treatment (e.g., Natural, Spontaneous, Artificial). Replicate: Experimental replicate number. Fruit_with_seed: Presence of seeds in the fruit (0 = no seeds, 1 = seeds present). **2022_2023_Reproductive_systems_of_Hohenbergia_Number_of_Ovules.txt:** Provides the number of ovules per flower. Data are organized in a tabular format with columns: Species: Plant species. Replicate: Experimental replicate number. Ovule Count: Count of ovules per flower (units: count). **2022_2023_Reproductive_systems_of_Hohenbergia_Number_of_Pollen_Grains.txt:** Lists the number of pollen grains produced per flower. Data are organized in a tabular format with columns: Species: Plant species. Replicate: Experimental replicate number. Field: Counting field in a Neubauer chamber. Quadrant: Subdivision of the counting field. Pollen Grain Count: Count of pollen grains per quadrant (units: count). **2022_2023_Reproductive_systems_of_Hohenbergia_Seeds_per_Fruit.txt:** Presents the number of seeds per fruit. Data are organized in a tabular format with columns: Species: Plant species. Treatment: Pollination treatment (e.g., Natural, Spontaneous, Artificial). Replicate: Experimental replicate number. Seeds_per_fruit: Count of seeds per fruit (units: count). Definition of Variables: Species Name: Identifies the plant species used in the experiment. Treatment: Refers to the experimental conditions applied to the samples. Number of Replicates: The number of samples or experimental units subjected to the same treatment. This value allows for statistical analysis and the assessment of data variability. Field: Refers to the counting field in the Neubauer chamber, a defined area containing a specific quantity of cells or particles in suspension. Each field is numbered or identified to standardize the counts. Quadrant: Subdivision within the field of the Neubauer chamber. The chamber is composed of several quadrants, each with defined areas for particle counting. This subdivision allows for more precise counting and the calculation of representative averages. ## Data Sharing/Access NA