cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8041607.v3
title: Combining Multiple Observational Data Sources to Estimate Causal Effects

description: The era of big data has witnessed an increasing availability of multiple data sources for statistical analyses. We consider estimation of causal effects combining big main data with unmeasured confounders and smaller validation data with supplementary information on these confounders. Under the unconfoundedness assumption with completely observed confounders, the smaller validation data allow for constructing consistent estimators for causal effects, but the big main data can only give error-prone estimators in general. However, by leveraging the information in the big main data in a principled way, we can improve the estimation efficiencies yet preserve the consistencies of the initial estimators based solely on the validation data. Our framework applies to asymptotically normal estimators, including the commonly used regression imputation, weighting, and matching estimators, and does not require a correct specification of the model relating the unmeasured confounders to the observed variables. We also propose appropriate bootstrap procedures, which makes our method straightforward to implement using software routines for existing estimators. Supplementary materials for this article are available online.
DOI: 10.6084/m9.figshare.14332982
title: MYB117 is a negative regulator of flowering time in <i>Arabidopsis</i>

description: Plant flowering is crucial for the onset and progression of reproduction processes. The control of flowering time is a sophisticated system with multiple known regulatory mechanisms in plants. Here, we show that MYB117 participates in the flowering time regulation in <i>Arabidopsis</i> as <i>myb117</i> mutants exhibited early flowering phenotypes under long-day condition. Transcriptome analysis of <i>myb117</i> mutants revealed 410 differentially expressed genes between wild type and <i>myb117</i>-1 mutants, where selective genes including the <i>Flowering Locus T</i> (<i>FT)</i> were further confirmed by qRT-PCR analysis. Further, <i>in vivo</i> dual-luciferase and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) assays showed that MYB117 directly binds to the promoter of <i>FT</i> to suppress its expression. Taken together, we have revealed the transcriptome profile of <i>myb117</i> mutants and identified MYB117 as a negative regulator in controlling flowering time through regulating the expression of <i>FT</i> in <i>Arabidopsis</i>.
DOI: 10.6084/m9.figshare.22721764
title: Research on the Integrated Training Mode of Higher Art Education for the Deaf

description: Abstract: The development of higher education for the disabled is one of the most important indicators of the development of education for the disabled in a country, whether the support policies are perfect, and whether the social civilization is advanced. The results showed that deaf students with general school experience had better adaptive ability The integration model was more conducive to improve deaf students’ school adaptive ability and social interaction awareness. The deaf students had higher demand for professional guidance and there is room for further improvement of the integration-training model. Based on the above research results and the problems in practice, this paper gives the corresponding countermeasure suggestions. The paper suggests that we should build a “trinity” integration education model oriented to socially integrated talents in art applications and improve the management system of integration education We should also improve the deaf college students’ learning support system under the leadership of the school, modify and reconstruct the integration curriculum system of arts and crafts majors, and strengthen the support and guidance of the society for the construction of integration environment.
DOI: 10.6084/m9.figshare.14333074.v1
title: Effectiveness of the oral human attenuated pentavalent rotavirus vaccine (RotaTeq™) postlicensure: a meta-analysis—2006–2020

description: Rotavirus (RV), which causes RV-associated gastroenteritis (RVGE), has accounted for considerable morbidity. We aimed to assess the eﬀectiveness (VE) of the oral pentavalent RV vaccine (RotaTeq™) in real-world settings in children and infants with gastroenteritis. We performed a systematic search for peer-reviewed studies published between 1 January 2006 and 1 May 2020 and a meta-analysis to calculate the VE of RotaTeq™ vaccine. The primary outcome was the pooled three-dose vaccine VE. Stratified analysis of the vaccine VEs was performed according to dosages, study design, population age, socioeconomic status (SES), introduction condition, control group types, outcomes of RV disease, and RV strains. After screening 2359 unique records, 28 studies were included and meta-analyzed. The overall VE estimate was 84% (95% confidence interval [CI], 80–87%). Stratified analyses revealed a nonnegligible impact of factors such as study design and SES. Other factors did not show great impart to VE with no significant differences between groups. RotaTeq™ is effective against RV infection, especially in high-income countries. Adopting suitable study methods and expansion of RV surveillance in low-income regions is crucial to assess VE in real-life settings and provide feasible vaccine regimens to improve vaccine VE.
DOI: 10.6084/m9.figshare.7517477.v1
title: Mutation screening of the SLC26A4 gene in a cohort of 192 Chinese patients with congenital hypothyroidism

description: ABSTRACT Objective Pendred syndrome (PS) is an autosomal recessive disorder characterised by sensorineural hearing loss and thyroid dyshormonogenesis. It is caused by biallelic mutations in the SLC26A4 gene encoding for pendrin. Hypothyroidism in PS can be present from birth and therefore diagnosed by neonatal screening. The aim of this study was to examine the SLC26A4 mutation spectrum and prevalence among congenital hypothyroidism (CH) patients in the Guangxi Zhuang Autonomous Region of China and to establish how frequently PS causes hearing impairment in our patients with CH. Subjects and methods Blood samples were collected from 192 CH patients in Guangxi Zhuang Autonomous Region, China, and genomic DNA was extracted from peripheral blood leukocytes. All exons of the SLC26A4 gene together with their exon-intron boundaries were screened by next-generation sequencing. Patients with SLC26A4 mutations underwent a complete audiological evaluation including otoscopic examination, audiometry and morphological evaluation of the inner ear. Results Next generation sequencing analysis of SLC26A4 in 192 CH patients revealed five different heterozygous variations in eight individuals (8/192, 4%). The prevalence of SLC26A4 mutations was 4% among studied Chinese CH. Three of the eight were diagnosed as enlargement of the vestibular aqueduct (EVA), no PS were found in our 192 CH patients. The mutations included one novel missense variant p.P469S, as well as four known missense variants, namely p.V233L, p.M147I, p.V609G and p.D661E. Of the eight patients identified with SLC26A4 variations in our study, seven patients showed normal size/location of thyroid gland, and one patients showed a decreased size one. Conclusions The prevalence of SLC26A4 pathogenic variants was 4% among studied Chinese patients with CH. Our study expanded the SLC26A4 mutation spectrum, provided the best estimation of SLC26A4 mutation rate for Chinese CH patients and indicated the rarity of PS as a cause of CH.
DOI: 10.6084/m9.figshare.8041640.v1
title: Sodium chloride primes JA-independent defense against <i>Spodoptera litura</i> (Fabricius) larvae in <i>Arabidopsis thaliana</i>

description: Priming for better defense performance is an important strategy in acclimation to the ever-changing environment. In the present study, defense priming induced by sodium chloride at the seedling stage significantly increased the expression of defense gene <i>VSP2</i>, the content of total glucosinolates and the level of the reactive oxygen species in mature <i>Arabidopsis thaliana</i> plants after transferred into the stress-free environment. The previously primed plants could effectively resist the feeding of <i>Spodoptera litura</i> (Fabricius) larvae. Salt-priming enhanced defense of Arabidopsis plants in the absence of either <i>MYC2</i> or <i>AOS</i>, which encodes a critical transcription factor in JA-signaling and an important enzyme in JA biosynthesis, respectively. Our results supported the JA-independent defense primed by sodium chloride, as well as the elevated ROS and glucosinolate level in primed plants. In addition, the feasibility of using mild salt-priming to improve crop performance in field was proposed.
DOI: 10.6084/m9.figshare.12757100
title: Additional file 2 of Characteristics and reference ranges of CD4+T cell subpopulations among healthy adult Han Chinese in Shanxi Province, North China

description: Additional file 2 Figure S1. Analysis of lymphocyte subsets by flow cytometry. A: Gating strategy for T cells, CD4+T cells, CD8+T cells: a expressed as a single parameter of CD45 and scatter; b T cells (CD3+); c CD4+T cells (CD3+CD4+); d CD8+T cells (CD3+CD8+). B: Gating strategy for B cells and NK cells: a expressed as a single parameter of CD45 and scatter; b expressed as a single parameter of CD3− and scatter; c: B cells (CD3−CD19+); d: NK cells (CD3−CD16+CD56+). Figure S2. Analysis of CD4+T subpopulations by flow cytometry. A: Gating strategy for Th1 and Th17 cells: a expressed as a single parameter of CD4 and scatter; b Th1 (CD4+IFN-γ+); c Th17 (CD4+IL-17+). B: Gating strategy for Th2 cells: a expressed as a single parameter of CD4 and scatter; b Th2 (CD4+IL-4+). C: Gating strategy for Treg cells: a expressed as a single parameter of CD4 and scatter; b Treg (CD4+CD25+Foxp3+).
DOI: 10.6084/m9.figshare.14936106.v1
title: Additional file 1 of Full-length transcriptome analysis reveals the mechanism of acupuncture at PC6 improves cardiac function in myocardial ischemia model

description: Additional file 1: Table S1. The differentially expressed genes in MI group compared to Control group.
DOI: 10.6084/m9.figshare.19576056.v1
title: Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation

description: Macroautophagy/autophagy is a cellular and energy homeostatic mechanism that contributes to maintain the number of primordial follicles, germ cell survival, and anti-ovarian aging. However, it remains unknown whether autophagy in granulosa cells affects oocyte maturation. Here, we show a clear tendency of reduced autophagy level in human granulosa cells from women of advanced maternal age, implying a potential negative correlation between autophagy levels and oocyte quality. We therefore established a co-culture system and show that either pharmacological inhibition or genetic ablation of autophagy in granulosa cells negatively affect oocyte quality and fertilization ability. Moreover, our metabolomics analysis indicates that the adverse impact of autophagy impairment on oocyte quality is mediated by downregulated citrate levels, while exogenous supplementation of citrate can significantly restore the oocyte maturation. Mechanistically, we found that ACLY (ATP citrate lyase), which is a crucial enzyme catalyzing the cleavage of citrate, was preferentially associated with K63-linked ubiquitin chains and recognized by the autophagy receptor protein SQSTM1/p62 for selective autophagic degradation. In human follicles, the autophagy level in granulosa cells was downregulated with maternal aging, accompanied by decreased citrate in the follicular fluid, implying a potential correlation between citrate metabolism and oocyte quality. We also show that elevated citrate levels in porcine follicular fluid promote oocyte maturation. Collectively, our data reveal that autophagy in granulosa cells is a beneficial mechanism to maintain a certain degree of citrate by selectively targeting ACLY during oocyte maturation. <b>Abbreviations:</b> 3-MA: 3-methyladenine; ACLY: ATP citrate lyase; AMA: advanced maternal age; CG: cortical granule; CHX: cycloheximide; CQ: chloroquine; CS: citrate synthase; COCs: cumulus-oocyte-complexes; GCM: granulosa cell monolayer; GV: germinal vesicle; MII: metaphase II stage of meiosis; PB1: first polar body; ROS: reactive oxygen species; shRNA: small hairpin RNA; SQSTM1/p62: sequestosome 1; TCA: tricarboxylic acid; TOMM20/TOM20: translocase of outer mitochondrial membrane 20; UBA: ubiquitin-associated domain; Ub: ubiquitin; WT: wild-type
DOI: 10.6084/m9.figshare.7517516
title: Comparison of the effects of albumin and crystalloid on mortality among patients with septic shock: systematic review with meta-analysis and trial sequential analysis

description: ABSTRACT BACKGROUND: This study aimed to compare the effects on mortality of albumin and crystalloid, used for fluid resuscitation among adult patients with septic shock, through conducting a meta-analysis and trial sequential analysis (TSA). DESIGN AND SETTING: Meta-analysis and TSA conducted at Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China. METHODS: Data were collected from several major databases including MEDLINE, EMBASE, Clinical Trials.gov and Cochrane Central Register of Controlled Trials. Studies that compared the effects of albumin therapy versus crystalloid therapy on mortality among adult septic shock patients were eligible for inclusion in the analyses. The study name, year of publication, country of the trial, albumin concentration, type of crystalloid and all reported mortalities at different follow-up endpoints were extracted. RESULTS: Compared with crystalloid, albumin did not decrease all-cause mortality at the final follow-up. However, in TSA, the required information size was not achieved in all groups, which means that the effect size was not definitive and further RCTs are needed to confirm or deny these findings CONCLUSIONS: Compared with crystalloid solutions, albumin was unable to decrease all-cause mortality. However, TSA indicated that these results could be false-negative. Additional randomized controlled trials are needed to clarify this discrepancy.
DOI: 10.6084/m9.figshare.14332982.v1
title: MYB117 is a negative regulator of flowering time in <i>Arabidopsis</i>

description: Plant flowering is crucial for the onset and progression of reproduction processes. The control of flowering time is a sophisticated system with multiple known regulatory mechanisms in plants. Here, we show that MYB117 participates in the flowering time regulation in <i>Arabidopsis</i> as <i>myb117</i> mutants exhibited early flowering phenotypes under long-day condition. Transcriptome analysis of <i>myb117</i> mutants revealed 410 differentially expressed genes between wild type and <i>myb117</i>-1 mutants, where selective genes including the <i>Flowering Locus T</i> (<i>FT)</i> were further confirmed by qRT-PCR analysis. Further, <i>in vivo</i> dual-luciferase and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) assays showed that MYB117 directly binds to the promoter of <i>FT</i> to suppress its expression. Taken together, we have revealed the transcriptome profile of <i>myb117</i> mutants and identified MYB117 as a negative regulator in controlling flowering time through regulating the expression of <i>FT</i> in <i>Arabidopsis</i>.
DOI: 10.6084/m9.figshare.22721789
title: Baseline predictors of short-term visual outcomes after intravitreal conbercept injection for neovascular age-related macular degeneration

description: ABSTRACT Purpose: Neovascular age-related macular degeneration is the leading cause of vision loss in the elderly. We aimed to identify baseline predictors of visual prognosis after intravitreal conbercept injection for neovascular age-related macular degeneration. Methods: We conducted a retrospective review of 58 patients with neovascular age-related macular degeneration who were treated with intravitreal injections of conbercept 0.5 mg in routine clinical practice. Basic information such as age, sex, intraocular pressure, and disease course was collected. Best-corrected visual acuity, mean retinal sensitivity, and optical coherence tomography findings were recorded at baseline and 6 months after treatment. Logistic regression analysis was used to identify independent predictors of best-corrected visual acuity at 6 months after treatment. Results: After the 6-month treatment, the mean best-corrected visual acuity improved from 1.10 ± 0.42 logarithm of the minimum angle of resolution (logMAR) to 0.41 ± 0.18 logMAR, the mean retinal sensitivity increased from 5.13 ± 0.86 dB to 7.32 ± 1.21 dB, the mean central retinal thickness decreased from 440.38 ± 61.05 μm to 260.01 ± 24.86 μm, and the total number of hyperreflective dots and the number of hyperreflective dots in each retina layer were significantly reduced as compared with those before treatment (all p&lt;0.05). Twenty-two patients showed improved vision, and 36 had unimproved vision. Multivariate analyses revealed that the number of subretinal hyperreflective dots, the state of external limiting membrane, baseline best-corrected visual acuity, and age were independent predictors of best-corrected visual acuity (all p&lt;0.05). Conclusion: Poor recovery of patients after intravitreal conbercept injection may be related to the number of subretinal hyperreflective dots, the state of external limiting membrane, baseline best-corrected visual acuity, and age, which may be used as predictors of short-term visual outcomes and should be fully evaluated before operation.
DOI: 10.6084/m9.figshare.7517477
title: Mutation screening of the SLC26A4 gene in a cohort of 192 Chinese patients with congenital hypothyroidism

description: ABSTRACT Objective Pendred syndrome (PS) is an autosomal recessive disorder characterised by sensorineural hearing loss and thyroid dyshormonogenesis. It is caused by biallelic mutations in the SLC26A4 gene encoding for pendrin. Hypothyroidism in PS can be present from birth and therefore diagnosed by neonatal screening. The aim of this study was to examine the SLC26A4 mutation spectrum and prevalence among congenital hypothyroidism (CH) patients in the Guangxi Zhuang Autonomous Region of China and to establish how frequently PS causes hearing impairment in our patients with CH. Subjects and methods Blood samples were collected from 192 CH patients in Guangxi Zhuang Autonomous Region, China, and genomic DNA was extracted from peripheral blood leukocytes. All exons of the SLC26A4 gene together with their exon-intron boundaries were screened by next-generation sequencing. Patients with SLC26A4 mutations underwent a complete audiological evaluation including otoscopic examination, audiometry and morphological evaluation of the inner ear. Results Next generation sequencing analysis of SLC26A4 in 192 CH patients revealed five different heterozygous variations in eight individuals (8/192, 4%). The prevalence of SLC26A4 mutations was 4% among studied Chinese CH. Three of the eight were diagnosed as enlargement of the vestibular aqueduct (EVA), no PS were found in our 192 CH patients. The mutations included one novel missense variant p.P469S, as well as four known missense variants, namely p.V233L, p.M147I, p.V609G and p.D661E. Of the eight patients identified with SLC26A4 variations in our study, seven patients showed normal size/location of thyroid gland, and one patients showed a decreased size one. Conclusions The prevalence of SLC26A4 pathogenic variants was 4% among studied Chinese patients with CH. Our study expanded the SLC26A4 mutation spectrum, provided the best estimation of SLC26A4 mutation rate for Chinese CH patients and indicated the rarity of PS as a cause of CH.
DOI: 10.6084/m9.figshare.8041607.v1
title: Combining multiple observational data sources to estimate causal effects

description: The era of big data has witnessed an increasing availability of multiple data sources for statistical analyses. We consider estimation of causal effects combining big main data with unmeasured confounders and smaller validation data with supplementary information on these confounders. Under the unconfoundedness assumption with completely observed confounders, the smaller validation data allow for constructing consistent estimators for causal effects, but the big main data can only give error-prone estimators in general. However, by leveraging the information in the big main data in a principled way, we can improve the estimation efficiencies yet preserve the consistencies of the initial estimators based solely on the validation data. Our framework applies to asymptotically normal estimators, including the commonly-used regression imputation, weighting, and matching estimators, and does not require a correct specification of the model relating the unmeasured confounders to the observed variables. We also propose appropriate bootstrap procedures, which makes our method straightforward to implement using software routines for existing estimators.
DOI: 10.6084/m9.figshare.8041607.v2
title: Combining Multiple Observational Data Sources to Estimate Causal Effects

description: The era of big data has witnessed an increasing availability of multiple data sources for statistical analyses. We consider estimation of causal effects combining big main data with unmeasured confounders and smaller validation data with supplementary information on these confounders. Under the unconfoundedness assumption with completely observed confounders, the smaller validation data allow for constructing consistent estimators for causal effects, but the big main data can only give error-prone estimators in general. However, by leveraging the information in the big main data in a principled way, we can improve the estimation efficiencies yet preserve the consistencies of the initial estimators based solely on the validation data. Our framework applies to asymptotically normal estimators, including the commonly used regression imputation, weighting, and matching estimators, and does not require a correct specification of the model relating the unmeasured confounders to the observed variables. We also propose appropriate bootstrap procedures, which makes our method straightforward to implement using software routines for existing estimators. Supplementary materials for this article are available online.
DOI: 10.6084/m9.figshare.22721764.v1
title: Research on the Integrated Training Mode of Higher Art Education for the Deaf

description: Abstract: The development of higher education for the disabled is one of the most important indicators of the development of education for the disabled in a country, whether the support policies are perfect, and whether the social civilization is advanced. The results showed that deaf students with general school experience had better adaptive ability The integration model was more conducive to improve deaf students’ school adaptive ability and social interaction awareness. The deaf students had higher demand for professional guidance and there is room for further improvement of the integration-training model. Based on the above research results and the problems in practice, this paper gives the corresponding countermeasure suggestions. The paper suggests that we should build a “trinity” integration education model oriented to socially integrated talents in art applications and improve the management system of integration education We should also improve the deaf college students’ learning support system under the leadership of the school, modify and reconstruct the integration curriculum system of arts and crafts majors, and strengthen the support and guidance of the society for the construction of integration environment.