cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/a26jd-40t15
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-33/rawdata/</p>dH_LG_64step2_19.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-33/rawdata/dH_LG_64step2_19.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2brcw-mh687
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-24/rawdata/</p>PseudoJT2944Tilt33.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-24/rawdata/PseudoJT2944Tilt33.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rgb7w-cf866
title: bio12-2_lam3_ts4

description: IT 0.8s
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 3°, constant angular increment, dosage: 88.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-25/rawdata/</p>bio12-2_lam3_ts4.st,  Tilt Series (Pixel Size 0.48 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-25/rawdata/bio12-2_lam3_ts4.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/79fvb-vyn79
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-96/rawdata/</p>Pseudomonas_JT2944Tilt521.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-96/rawdata/Pseudomonas_JT2944Tilt521.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7wd3cm
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Chiloglanis carnosus, Chiloglanis congicus, Chiloglanis deckenii, Chiloglanis devosi, Chiloglanis dialloi, Chiloglanis disneyi, Chiloglanis elisabethianus, Chiloglanis emarginatus, Chiloglanis fasciatus, Chiloglanis harbinger, Chiloglanis kabaensis, Chiloglanis kalambo, Chiloglanis kerioensis, Chiloglanis kolente, Chiloglanis lamottei, Chiloglanis loffabrevum, Chiloglanis longibarbis, Chiloglanis lufirae, Chiloglanis lukugae, Chiloglanis macropterus, Chiloglanis marlieri, Chiloglanis mbozi, Chiloglanis microps, Chiloglanis modjensis, Chiloglanis mongoensis, Chiloglanis neumanni, Chiloglanis niger, Chiloglanis niloticus, Chiloglanis normani, Chiloglanis nzerekore, Chiloglanis occidentalis, Chiloglanis paratus, Chiloglanis pezoldi, Chiloglanis pojeri, Chiloglanis polyodon, Chiloglanis polypogon, Chiloglanis pretoriae, Chiloglanis reticulatus, Chiloglanis rukwaensis, Chiloglanis ruziziensis, Chiloglanis sanagaensis, Chiloglanis sardinhai, Chiloglanis somereni, Chiloglanis swierstrai, Chiloglanis trilobatus, Chiloglanis tweddlei, Chiloglanis voltae, Chiloglanis waterloti, Chilotilapia euchilus, Chilotilapia rhoadesii, Chindongo ater, Chindongo bellicosus, Chindongo cyaneus, Chindongo demasoni, Chindongo flavus, Chindongo heteropictus, Maylandia heteropicta, Chindongo longior, Chindongo minutus, Chindongo saulosi, Chromidotilapia cavalliensis, Chromidotilapia elongata, Chromidotilapia guntheri, Chromidotilapia kingsleyae, Chromidotilapia linkei, Chromidotilapia mamonekenei, Chromidotilapia melaniae, Chromidotilapia mrac, Chromidotilapia nana, Chromidotilapia regani, Chromidotilapia schoutedeni, Chrysichthys aluuensis, Chrysichthys ansorgii, Chrysichthys auratus, Chrysichthys bocagii, Chrysichthys brachynema, Chrysichthys brevibarbis, Chrysichthys cranchii, Chrysichthys dageti, Chrysichthys delhezi, Chrysichthys dendrophorus, Chrysichthys duttoni, Chrysichthys furcatus, Chrysichthys nigrodigitatus, Chrysichthys grandis, Chrysichthys graueri, Chrysichthys habereri, Chrysichthys helicophagus, Chrysichthys hildae, Chrysichthys johnelsi, Chrysichthys laticeps, Chrysichthys levequei, Chrysichthys longibarbis, Chrysichthys longidorsalis, Chrysichthys longipinnis, Chrysichthys mabusi, Chrysichthys macropterus, Chrysichthys maurus, Chrysichthys nyongensis)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((46.8009 -14.7158,46.1382 -15.1614,46.1382 -15.9401,50.8081 -15.9401,50.9712 -15.4415,50.3203 -12.5851,49.5255 -11.523,49.0875 -11.4772,48.2794 -11.911,48.1654 -12.5557,47.3945 -13.3102,47.2457 -14.0495,46.8009 -14.7158))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006518-250827131500795/datasets/export for details.
DOI: 10.22002/r59gf-k5m69
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-17</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-82/rawdata/</p>targ80.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-82/rawdata/targ80.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qktpk-msn73
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-62/rawdata/</p>dH_LG_64step2_33.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-62/rawdata/dH_LG_64step2_33.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jkyyq-mta38
title: Pombe Cryosections

description: These cells were high pressure frozen after the majority of the rings were around mid-division.
description: <strong>Tilt Series Date:</strong> 2015-06-03</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Manual/Raptor</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-06-03-6/rawdata/</p>060315_pombe_cryosection2_square3_atlas.mrc,  Tilt Series (Pixel Size 0.5 nm), 4.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n0dm3-fvp45
title: Pombe Cryosections

description: These cells were high pressure frozen after the majority of the rings were around mid-division.
description: <strong>Tilt Series Date:</strong> 2015-06-03</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Manual/Raptor</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-06-03-1/rawdata/</p>060315_pombe_cryosection2_square1_atlas.mrc,  Tilt Series (Pixel Size 0.5 nm), 4.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zx3wj-he943
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-83/rawdata/</p>dH_LG_64step2_54.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-83/rawdata/dH_LG_64step2_54.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/51qt1-9h969
title: B. diazoefficiens, hopanoids

description: WT strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-56/rawdata/</p>WT_LG_64step2_8.mrc,  Tilt Series (Pixel Size 2.678 nm), 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-56/rawdata/WT_LG_64step2_8.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7svbjf
title: Occurrence Download

description: A dataset containing 1643 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Nissolia vincentina"
 ]
}

 The dataset includes 1643 records from 155 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006519-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/qf8kf-t9n84
title: RalF-Ub V76

description: Lp02 pCyaA::RalF-Ub V76 free from U937
description: <strong>Tilt Series Date:</strong> 2020-07-31</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Stephen Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, 1-2 washes, fibronectin/vitronectin/no matrix. Legionella: 2.5 O.D, 3h after 200mM  IPTG induction, at O.D. 3</p>
<strong>Sample Preparation:</strong> 200mesh Gold quantifoil,  10nm gold, 37deg C, 80-90% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-89/rawdata/</p>RalFUbV76_244.mrc,  Tilt Series (Pixel Size 0.265 nm), 235.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-89/rawdata/RalFUbV76_244.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xyg5y-mw088
title: bio12-2_lam6_atlas

description: .  [added 2019-10-14 12:31:18] High reZ Atlas of the lamella done at 19500x mag, with preview settings to keep the dose low (0.096 exposure time)
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 0.0 μm,  magnification: 0x. </p>
<strong>Microscope:</strong> 0</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> </p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-31/rawdata/</p>atlas_lam6.mrc,  Tilt Series (Pixel Size 0.0 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-31/rawdata/atlas_lam6.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vqxc0-dak09
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-229/rawdata/</p>JV9298_PD-1-3_103.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
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