DOI: 10.5061/dryad.18931zd8v
title: Data from: Temperature-dependent shifts in multiple tri-trophic defense interactions on black cherry

description: Many plants engage in tri-trophic interactions whereby plants provide
 resources such as food or shelter to mutualists in exchange for protection
 against herbivores and pathogens. Despite their ecological importance,
 tri-trophic interactions are often context dependent, in which the
 magnitude and outcome of the interaction can shift under different abiotic
 and biotic conditions. Disentangling the effects of environmental factors,
 such as temperature, on tri-trophic interactions is especially important
 as temperature regimes shift under climate change, but few studies have
 explored how abiotic shifts affect tri-trophic interactions across
 multiple trophic groups in the same plant system. Here, we monitored 96
 black cherry (Prunus serotina) seedlings for 10 weeks under ambient and
 increased temperatures to explore the effects of temperature on
 interactions across multiple trophic levels. Specifically, we explored the
 effects of temperature on two different tri-trophic interactions between
 black cherry and: (1) mutualistic leaf domatia-dwelling mites and leaf
 fungi; and (2) arthropod predators and herbivores. We present two
 datasets: one contains data on mites (count), leaf fungi (count of hyphae
 along a transect), leaf domatia (domatia size in milimeters), and
 extrafloral nectaries (count), collected at the end of the experiment from
 a subset of 3 leaves per plant. The next contains data on herbivory
 (proportion of leaves with evidence of herbivory on each plant), predator
 abundance (count of predators seen each week), and plant size (height),
 collected weekly over the 10 week experiment. We also present the code
 used to process and analyze these datasets, with the results presented in
 our main manuscript "Temperature-dependent shifts in multiple
 tri-trophic defense interactions on black cherry."
description: # Data from: Temperature-dependent shifts in multiple tri-trophic defense
 interactions on black cherry Dataset DOI:
 [10.5061/dryad.18931zd8v](10.5061/dryad.18931zd8v) ## Description of the
 data and file structure In June-August 2018, we grew 96 *Prunus serotina*
 seedlings under either elevated temperature or ambient temperature
 conditions. Over the course of 10 weeks, we weekly measured the abundance
 of arthopod predators (spiders and ants), the amount of herbivory plants
 experienced, and plant height. At the conclusion of the experiment, we
 collected a subset of 3 leaves per plant and assessed mite abundance, the
 abundance of fungal hyphae, the presence and size of leaf domatia, and
 extrafloral nectary abundance on each leaf. We combine these datasets to
 assess the effect of warming on the abundances and interactions between
 these groups. ### Files and variables #### File:
 Dawson-Glass_warming_cherries_analyses_070125.R **Description:** Script
 with data preparation and statistical analyses performed on the collected
 data. #### File: leafleveldata070225.csv **Description:** Data that was
 collected from a subset of 3 leaves per plant. Missing values are
 indicated by NA. ##### Variables * ring: ID value of the heating ring plot
 at the Kellogg Biological Station that the seedling was planted into. *
 plant: Plant ID number. * domatia.presence: Binary value of if a leaf
 domatia was present on the collected leaf. A value of 1 indicates domatia
 is present, and 0 indicates domatia is absent. * fungal.count: The number
 of fungal hyphae strands on the leaf, assessed using a tape peel that
 removed hyphae from the leaf surface, that crossed a transect of the tape
 along the 19 mm width of the tape. * mite.count: The number of mites found
 on the leaf. * notes: Any notes about the replicate that might be
 relevant. Notes include "not present" which indicates that this
 leaf replicate was not available for sampling (because of an insufficient
 number of leaves on the plant) and "no stem" which means the
 petiole of the leaf was absent or damaged, preventing us from counting the
 number of extrafloral nectaries on the leaf.  * rep: Leaf replicate
 (either 1, 2, or 3). * treatment: Warmed or control treatment. *
 dom.length.mm: The size of leaf domatia, in milimeters. * leaf.length.cm:
 Leaf replicate size, in centimeters. * EFN: Extrafloral nectary count.
 #### File: Prunusweelkydatafull070225.csv **Description:** Data that was
 collected weekly from the *Prunus serotina* seedlings. Missing values are
 indicated by NA. ##### Variables * Ring.Plant: Plant ID, which is a
 concatenation of the plant replicate ID number and the heating ring ID
 number. * Treatment: Warmed or control treatment. * week: Survey week.  *
 plot: ID value of the heating ring plot at the Kellogg Biological Station
 that the seedling was planted into. * Height: Plant height in centimeters.
 * Number.of.Leaves: The number of leaves on the plant.  * Number.of.Ants:
 The number of ants seen on the plant. * Number.of.Spiders: The number of
 spiders seen on the plant. * Number.of.Herbivory: The number of leaves
 showing signs of herbivory. * Notes: Any notes about the seedling at the
 time of data collection. ## Code/software **File:**
 Dawson-Glass_warming_cherries_analyses_070125.R **Description:** Script
 with data preparation and statistical analyses performed on the collected
 data. Working directory must be assigned before reading in the previously
 described data files and running the script. **Software:** R version 4.3.1
 **Packages used:**  dplyr ggplot2 lme4 emmeans ggpubr piecewiseSEM nlme
 tidyr arm ####