cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/d3rkv-byh75
title: Sulfolobus solfac.P2+STIV - 41KX

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 10 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-02-05</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-05-15/rawdata/</p>atlas8stiv100006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-05-15/rawdata/atlas8stiv100006.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas8stiv100006_part111_34.rec,  Reconstruction (Pixel Size 1.56 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-05-15/3dimage_38444/atlas8stiv100006_part111_34.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9g61w-gb282
title: Vibrio cholerae MKW1383

description: In the mixture with Bdellovibrio for 16 hr at 30C.
description: <strong>Tilt Series Date:</strong> 2013-11-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 16 hr at 30 C before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu R2/2 200 grid</p>
  ycw2013-11-06-21_slicer9428.jpg: Vibrio cholerae .  comb ycw2013-11-06-21_slicer9429.jpg: Vibrio cholerae .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-06-21/rawdata/</p>01-montage_7x70010.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-06-21/rawdata/01-montage_7x70010.mrc"
        > <i class="download icon"></i> Download </a></p>

        01-montage_7x70010_part101_10.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-06-21/3dimage_34161/01-montage_7x70010_part101_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ghktg-5jj62
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC101
description: <strong>Tilt Series Date:</strong> 2014-05-13</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-05-13-14_slicer5545.jpg: Pilli ycw2014-05-13-14_slicer5548.jpg: Unknown appendage ycw2014-05-13-14_slicer5552.jpg: Unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-13-14/3dimage_42459/</p>20140513_YWC101_20007_part131_5.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-13-14/3dimage_42459/20140513_YWC101_20007_part131_5.rec"
        > <i class="download icon"></i> Download </a></p>

        20140513_YWC101_20007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-13-14/rawdata/20140513_YWC101_20007.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0j7ev-eaj65
title: H.pylori 26695 WT

description: Grid box YWC089.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-01-7_slicer17945.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-7/rawdata/</p>20140301_YWC089_10007.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-7/rawdata/20140301_YWC089_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140301_YWC089_10007_part131_4.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-7/3dimage_39417/20140301_YWC089_10007_part131_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xjxvy-7xr83
title: Myxococcus xanthus DK1622 wt

description: For T4P averaging
description: <strong>Tilt Series Date:</strong> 2013-08-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-08-26-87_slicer19556.jpg: tip of T4P ycw2013-08-26-87_slicer19557.jpg: tip of T4P
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-87/rawdata/</p>20130826_DK1622_WT_30029.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-87/rawdata/20130826_DK1622_WT_30029.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130826_DK1622_WT_30029_part131_2.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-87/3dimage_31518/20130826_DK1622_WT_30029_part131_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c5rkv-q5t79
title: Vibrio cholerae MKW1383

description: Vibrio cholerae MKW1383 in the mixture with Bdellovibrio.Imaged by Janelia farm Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-28/rawdata/</p>grid3-tiltseries0028.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-28/rawdata/grid3-tiltseries0028.mrc"
        > <i class="download icon"></i> Download </a></p>

        grid3-tiltseries0028_full.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-28/3dimage_26423/grid3-tiltseries0028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bs1cb-c2p48
title: Vibrio cholerae MKW1383

description: In the mixture with Bdellovibrio for 4 hr at 30C. Cells seemed lost PG layer without Bdellovibrio inside.
description: <strong>Tilt Series Date:</strong> 2013-10-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 4 hr at 30 C before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
  ycw2013-10-09-40_slicer9848.jpg: Vibrio cholerae .  DNA bundles?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-40/rawdata/</p>montage_9x90013.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-40/rawdata/montage_9x90013.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_9x90013_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-40/3dimage_32522/montage_9x90013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9s296-p1739
title: H. pylori 26695 / Hs.746T

description: Grid box YWC057
description: <strong>Tilt Series Date:</strong> 2013-12-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-43/rawdata/</p>20131216_YWC057_20003.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-43/rawdata/20131216_YWC057_20003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131216_YWC057_20003_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-43/3dimage_36192/20131216_YWC057_20003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cy0rf-qc396
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2013-08-20</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett, Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-41/rawdata/</p>20130820_Bdellovibrio+WM3433_10032.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-41/rawdata/20130820_Bdellovibrio+WM3433_10032.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130820_Bdellovibrio+WM3433_10032_part131_2.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-41/3dimage_29924/20130820_Bdellovibrio+WM3433_10032_part131_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/02ab7-tzk73
title: Myxococcus xanthus pilM-YFP

description: strain SA4088grid box YWC101
description: <strong>Tilt Series Date:</strong> 2014-05-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-05-16-9_slicer6858.jpg: pilli ycw2014-05-16-9_slicer6861.jpg: pilli ycw2014-05-16-9_slicer6863.jpg: External vesicle ycw2014-05-16-9_slicer6864.jpg: fillament ycw2014-05-16-9_slicer6865.jpg: fillament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-9/rawdata/</p>20140516_Janelia_YWC096_10036.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-9/rawdata/20140516_Janelia_YWC096_10036.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140516_Janelia_YWC096_10036_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-9/3dimage_42750/20140516_Janelia_YWC096_10036_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25919/dnxx-8942
title: A Standard Metadata Template for Representing Soil Specimens

description: This dataset contains a standard template for representing the metadata of soil specimens in the CSIRO Mineral Resources Discovery program. The template includes core properties of samples such as their name, identifier, type, and location, as well as associated metadata such as project, drilling contexts, hazard declaration and physical storage. 
The template will be used to catalogue legacy and specimens systematically collected through mineral exploration projects. It has been developed iteratively, revised, and improved based on feedback from researchers and lab technicians. This standardized template can prevent duplicate sample metadata entry and lower metadata redundancy, thereby improving the program's physical sample curation and discovery.
DOI: 10.22002/1zp3s-0j642
title: A413T cooverexpression

description: co overexpression strain UU1607/pJC3/pPM25  with A413T mutation in tsr (mutation made by Cathereine, strain and plasmids published. Sent from Heather Hodges (Kiessling lab)
description: <strong>Tilt Series Date:</strong> 2013-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew UU1607/pJC3/pPM25 overnight in 5mL TB + 3.8µl Cm + 5µl CarbDiluted back 1:50 into 200mL TB + 152µl Cm + 200µl Carb + 50µl 1M IPTG + 200µl Na-salicylateGrew ~2 hours to mid-log phaseSpun down 10' at 5,000 x gResuspended in 300µl lysis buffer + 0.1µl PMSF + 0.75 µl 1,10-phenanthrolineAdded 33.3µl (2mg/ml) fresh lysozyme solution (20mg/ml in 10mM Tris-HCl, pH 8.0)Incubated 45' at 37°CAdded 1.67µl 1M MgCl2, 0.2µl 1M CaCl2, 0.33µl DNase IIncubated 30' at 37°CSpun down 4000 g 5 min Resuspended in activity buffer (cold room) without glycerol</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-11/rawdata/</p>A413tCoover0011.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
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        A413tCoover0011.rec,  Reconstruction (Pixel Size 1.28 nm), 1.4 GB  
        
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