DOI: 10.5061/dryad.2ch6ks8
title: Data from: Inbreeding diminishes herbivore‐induced metabolic responses in native and invasive plant populations

description: 1. Inbreeding and herbivory can interactively reduce the performance of
 flowering plants. Here, we investigated whether the magnitude of plant
 inbreeding depression increases under herbivory as a result of diminished
 leaf metabolic responses to herbivory in inbreds, which entails increased
 herbivore growth and feeding damage. We additionally explored whether
 genetic differentiation among native and invasive plant populations
 impacts the magnitude and direction of these inbreeding × herbivory
 interactions. 2. Inbred and outbred plants from eight European (native)
 and eight North American (invasive) populations of Silene latifolia were
 exposed to two consecutive control or herbivory treatments (infestation
 with larvae of Mamestra brassicae). After the first treatment, leaf
 metabolic responses to herbivory were assessed with untargeted metabolic
 fingerprinting using UHPLC‐ESI+‐QTOF‐MS/MS. After the second herbivory
 treatment, herbivore growth and plant feeding damage were assessed.
 Moreover, plant performance traits were recorded four weeks after the
 second herbivory treatment. 3. Metabolic fingerprinting revealed that
 changes in the pool sizes of various metabolic features in response to
 herbivory were less intense in inbred than outbred plants from native and
 invasive populations. While herbivore growth and feeding damage were
 largely unaffected by plant breeding status in invasive populations,
 herbivores consumed greater biomass from, but showed reduced growth on
 inbred than outbred native plants. The magnitude of inbreeding depression
 in plant performance traits was not affected by herbivory, but was
 partially higher in invasive compared to native populations. 4. Synthesis:
 The chemical analyses suggest that inbreeding compromises
 herbivory‐induced metabolic defenses in S. latifolia, while results for
 herbivore growth and feeding damage indicate that inbreeding
 simultaneously reduces the nutritional quality of plants. Furthermore, our
 findings demonstrate that the magnitude and direction of these inbreeding
 effects can be shaped by genetic differentiation in metabolic responses
 among native and invasive plants. These range‐specific responses highlight
 the need to investigate multiple populations with different evolutionary
 histories to gain a deeper understanding of inbreeding × herbivory
 interactions and provide novel insights into the role of these
 interactions in invasion success.
description: D.A: Plant metabolic
 responses to herbivoryThe table provides the
 following information for each of the Silene latifolia individuals in the
 experiment: distribution range (range; native: eu, invasive: us),
 population affiliation (corresponds to population IDs in Fig. S1, and
 Table S2), maternal family of the mother in the first generation crossing
 (maternal_family, a-e), applied breeding treatment (breeding_tmt;
 outbreeding: o, inbreeding: i), applied herbivory treatment
 (herbivory_tmt; control: c, herbivory: h) and the intensity (i.e., peak
 area) of each metabolic feature (characterized by retention time [min] and
 mass/charge ratio [m/z]).Plant metabolic responses to
 herbivory.xlsxD.B: Herbivore growth,
 feeding damage, plant performanceThe table provides the
 following information for each of the Silene latifolia individuals in the
 experiment: distribution range (range; native: eu, invasive: us),
 population affiliation (corresponds to population IDs in Fig. S1, and
 Table S2), maternal family of the mother in the first generation crossing
 (family_mother, a-e), maternal family of the father in the first
 generation crossing (family_father, a-e), applied breeding treatment
 (breeding_tmt; outbreeding: o, inbreeding: i), applied herbivory treatment
 (herbivory_tmt; control: c, herbivory: h), start weight of the Mamestra
 brassicae larva (start_weight_larva, in mg, NA for control plants), end
 weight of the Mamestra brassicae larva (end_weight_larva, in mg, NA for
 control plants), number of days the larva fed on the plant (no_days_fed,
 NA for control plants), leaf area removed from the plant by the larva
 (consumed_leaf_area, in mm², NA for control plants), dry weight per mm²
 leaf (leaf_density, mg/mm², NA for control plants), dry plant biomass
 consumed by the larva (consumed_biomass, in mg, NA for control plants),
 number of leaves at the plant age of 100 d (no_leaves_t3, NA for plants
 that died after the herbivory treatment), leaf length at the plant age of
 100 d (leaf_length_t3, in cm, NA for plants that died after the herbivory
 treatment), leaf width at the plant age of 100 d (leaf_width_t3, in cm, NA
 for plants that died after the herbivory treatment), presence of side
 shoots at the plant age of 100 d (binary variable with two columns:
 buds_yes_t3 and buds_no_t3, NA for plants that died after the herbivory
 treatment), presence of flowers at the plant age of 100 d (binary variable
 with two columns: flower_yes_t3 and flower_no_t3, NA for plants that died
 after the herbivory treatment) and dry aboveground biomass
 (plant_biomass_t3, in mg, NA for plants that died after the herbivory
 treatment).Herbivore growth, feeding
 damage, plant performance.xlsx