DOI: 10.22002/v2h59-yk910
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-12-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-8/rawdata/</p>11dec22_C104_1_AT3BT1_0006.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        11dec22_C104_1_AT3BT1_0006_full.rec,  Reconstruction (Pixel Size 2.0 nm), 483.1 MB  
        
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        11dec22_C104_1_AT3BT1_0006_ClathEndo.tiff,  file associated to 3D Image #68115, 761.2 kB  
        
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        11dec22_C104_1_AT3BT1_0006_earlyEndoFusn.tiff,  file associated to 3D Image #68115, 801.0 kB  
        
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        11dec22_C104_1_AT3BT1_0006_full.tiff,  file associated to 3D Image #68115, 1.3 MB  
        
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        earlyendotubulation.tiff,  file associated to 3D Image #68115, 1.0 MB  
        
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