DOI: 10.5061/dryad.msbcc2g3q
title: Data from: Adaptive responses and transgenerational plasticity of a submerged plant to benthivorous fish disturbance

description: Benthivorous fish dominance in some subtropical lakes may alter submerged
 plant growth, reproduction, and progeny germination by changing
 water-submerged plant-algae interactions. We conducted mesocosm
 experiments to examine the effects of three densities of benthivorous
 fish, Misgurnus anguillicaudatus, on the water properties, the growth,
 asexual reproduction, and the germination of turions of Potamogeton
 crispus L. The dataset includes water environment characteristics,
 macrophyte traits, algae traits, and sediment nutrient characteristics.
 Macrophyte traits include morphological, stoichiometric, turion traits,
 and germination traits.
description: Macrophytes and sediment samples: All plants were harvested and
 carefully washed with tap water for later measurements. The branching
 number of <em>P. crispus</em> in each tank was counted.
 Measuring shoot length and root length with a straightedge (cm). All the
 plants were divided into leaf, root, stem, and turion. To determine the
 dry weight (DW), all leaves, roots, and stems were dried with bibulous
 paper, weighed, oven-dried at 70 °C to constant weight, and weighed (g).
 Twenty turions of each tank were randomly selected, oven-dried at 70 °C to
 constant weight, and weighed to determine the dry weight (DW). The
 remaining biomass was calculated according to the relative proportion. In
 each tank, the total biomass of each species was the sum of the DW of each
 plant organ. The dried plant and sediment samples were meticulously ground
 into a fine powder to determine C, N, and P. An elemental analyzer
 (UNICUBE, Elementar, Germany) was then used to analyze the N and C content
 in both plant tissues and sediment. The P content of the macrophyte and
 sediment was determined using a sulfuric acid-hydrogen peroxide digestion
 and ammonium molybdate–antimony potassium tartrate–ascorbic acid
 spectrophotometric method. The leaf chlorophyll concentration was assessed
 from 0.5 g fresh and ripe leaves plucked from the petiole on the final
 sampling day; the samples were put in 10 mL of 80 percent acetone in the
 dark for 48 hours at 25 C and then quantified by colorimetric analysis
 using a spectrophotometer. Periphyton biomass: The PE
 board was carefully removed by hand from each mesocosm and placed in a
 zip-lock plastic bag to analyze periphyton biomass in the laboratory. The
 periphyton (a mixture of algae, bacteria, and residue, but primarily
 algae) attached to the board was rinsed with distilled water and filtered
 through pre-combusted and pre-weighed GF/C filters, and subsequently dried
 in the oven at 70 °C for 48 hours. Phytoplankton
 biomass: Chlorophyll a (Chl-a) was measured with a handheld chlorophyll
 fluorometer probe (HYDROLAB DS5, HACH, United States) in the field
 tests. Water properties: We collected 1 L water samples
 from each tank with depth integration for chemical analysis. Total
 phosphorus (TP) was analyzed by the Total Phosphorus Analyzer (IL500P,
 HACH, USA). The colorimetric method was used to measure TN by following
 digestion with K2S2O8 and NaOH solution. The colorimetric method using
 Nessler's reagent was used to determine ammonia nitrogen (NH4-N),
 while the UV spectrophotometric method was used to assess nitrate nitrogen
 (NO3-N)  (Moss et al., 1996). PO4-P was determined by the molybdenum blue
 method (Gaudet, 1971). Photosynthetic active radiation (PAR) was measured
 at the water depth of 0 and 0.5 m (PAR1 and PAR2) using an underwater
 radiation sensor (UWQ-192S) connected to a data logger (Li-1400; Li-Cor
 Company, Lincoln, NE, U.S.A).  Germination traits: At
 the end of the experiment, all turions were harvested and were considered
 to have sprouted if at least one bud body appeared
 (&gt;1cm). Moss, B., J. Stansfield, K. Irvine, M.
 Perrow, and G. Phillips (1996) Progressive restoration of a shallow lake:
 A 12-year experiment in isolation, sediment removal and biomanipulation.
 Journal of Applied Ecology 33<strong>:</strong>71-86. https://doi.org/10.2307/2405017 Gaudet, J. J. (1971) Methods for chemical analysis of fresh waters. H. L. Golterman. The Quarterly Review of Biology. 
description: The dataset includes water environment characteristics, macrophyte traits,
 algae traits, and sediment nutrient characteristics. There are no missing
 values in this dataset. Information of table heads Table plant traits:
 ID-sample ID, FD- Fish density, column heads C1 to T1 was plant traits
 Table plant CNP: ID-sample ID, FD-Fish density, organ-plant organs, column
 heads D1 to K1 was Chemical stoichiometry characteristics Table water FD:
 ID-sample ID, FD-Fish density, Time- Sampling batches, TN-total nitrogen
 (mg·L-1), TP-total phosphorus (mg·L-1), NH3-N-ammonia nitrogen (mg·L-1),
 NO3-N- nitrate nitrogen (mg·L-1), PO4-P- orthophosphate (mg·L-1), Chla-
 Chl-a (μg·L-1). PAR1 and PAR2- Photosynthetic active radiation (μmol m−2
 s−1), K- Light extinction coefficient. Table water MD: ID-sample ID,
 MD-Fish density, Time- Sampling batches, TN-total nitrogen (mg·L-1),
 TP-total phosphorus (mg·L-1), NH3-N-ammonia nitrogen (mg·L-1), NO3-N-
 nitrate nitrogen (mg·L-1), PO4-P- orthophosphate (mg·L-1), Chla- Chl-a
 (μg·L-1). PAR1 and PAR2- Photosynthetic active radiation (μmol m−2 s−1),
 K- Light extinction coefficient. Table Periphyton: ID-sample ID, FD-Fish
 density, PB-Periphyton biomass (g·m-2). Table soil NP: ID-sample ID,
 FD-Fish density, column heads C1 to D1 was Soil P and Soil N (mg·g-1).
 Table germination rate: FD- Fish density when collecting turion, MD- Fish
 density for emergence experiments, GR- The final germination rate of
 turion . Table turion traits: ID-sample ID, FD-Fish density, TW-Turion
 weight (g)