DOI: 10.5061/dryad.qbzkh18rw
title: The genetic basis of incipient sexual isolation in Drosophila melanogaster

description: Speciation is a fundamental evolutionary process, but the genetic changes
 accompanying speciation are difficult to determine since true species do
 not produce viable and fertile offspring. Partially reproductively
 isolated incipient species are useful for assessing genetic changes that
 occur prior to speciation. Drosophila melanogaster from Zimbabwe, Africa
 are partially sexually isolated from other D. melanogaster populations
 whose males have poor mating success with Zimbabwe females. We used the
 North American D. melanogaster Genetic Reference Panel (DGRP) to show that
 there is significant genetic variation in the mating success of DGRP males
 with Zimbabwe females, to map genetic variants and genes associated with
 variation in mating success, and to determine whether mating success to
 Zimbabwe females is associated with other quantitative traits previously
 measured in the DGRP. Incipient sexual isolation is highly polygenic and
 associated with the common African inversion In(3R)K and the amount of the
 sex pheromone 5,9-heptacosadiene in DGRP females. We functionally
 validated the effect of eight candidate genes using RNA interference to
 provide testable hypotheses for future studies investigating the molecular
 genetic basis of incipient sexual isolation in D. melanogaster.
description: # The genetic basis of incipient sexual isolation in *Drosophila
 melanogaster* ## Description of data and files ### adjustedData.RData This
 RData contains the following objects: * adjustPheno: R function to adjust
 phenotype * inv: table for inversion genotypes where the row names are the
 line IDs and column names are the inversion names. In the table,
 "0" = reference karyotype; "1" = heterozygous for the
 inversion, "2" = homozygous for the inverted karyotype. * pcs:
 top 11 PCs for the genotypes where the row names are the line IDs and
 column names are the PC names * wolba: Wolbachia infection status where
 the row names are the line IDs and there is one column with "y"
 indicating infected lines and "n" indicating Wolbachia free. *
 writeCovar: returns a matrix of covariates, which can be used for other
 programs ### Z30mating.csv Z30 mating raw data, comma-separated file with
 the following columns: * series: series is similar to blocks where assays
 are performed in the same batch * female.line: female line in the mating
 assay * n.female: number of females in the assay * female.age: age of
 females * male.line: male line in the mating assay * n.male: number of
 males in the assay * male.age: age of males * mate.time1: time at which
 the first mating takes place * mate.time2: time at which the second mating
 takes place * mate.time3: time at which the third mating takes place *
 mate.time4: time at which the fourth mating takes place * mate.time5: time
 at which the fifth mating takes place * n.mate.1hr: number of females
 mated at 1 hour * n.mate.2hr: number of females mated at 2 hour *
 ratio.1hr: ratio of mated females at 1 hour * ratio.2hr: ratio of mated
 females at 2 hour NA's in this file represents missing data. For
 example, when only one fly mated, mate.time2, mate.time3, mate.time4, and
 mate.time5 will be NAs. ### z30.pheno Line means for mating ratio at 2
 hours, comma-separated file with the following columns (no column name): *
 Line: DGRP line IDs * LIne mean: line means for mating ratio at 2 hours