cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0h70dgr
title: CIL:38858, Arabidopsis thaliana, leaf. In Cell Image Library

description: Leaves from the arc5 plastid division mutant of Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ2-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin. The protein is localized at the site of division which occurs by constricting at the chloroplast mid-point. The upper image shows the fluorescence signal, the lower panel, the corresponding DIC image.
description: Preparation: formaldehyde fixed tissue; tissue in wax embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of epitope; boundaries between regions with different refractive index
Visualization methods: Fluorescein (FITC)
Data qualification: Processed
description: Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics. Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. See S. Vitha et al. 2001 J Cell Biol 153:111-119.
DOI: 10.6075/j0zk5fbr
title: CIL:38856, Arabidopsis thaliana, leaf. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; tissue in wax embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of epitope; boundaries between regions with different refractive index
Visualization methods: Fluorescein (FITC)
Data qualification: Processed
description: Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics. Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. See S. Vitha et al. 2001 J Cell Biol 153:111-119.
description: Leaves from wildtype Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ2-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin. The protein is localized at the site of division by constricting at the chloroplast mid-point. The upper images show the fluorescence signal, the lower panels, the corresponding DIC image.
DOI: 10.6075/j09z93rd
title: CIL:38809, Homo sapiens, blood cell. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
description: Scanning electron micrograph of a blood clot on the underside of a sticking plaster that has been used to treat a razor blade cut. Red blood cells (shown in red) and thin fibres of the protein fibrin (beige) can be seen between the gauze fibres of the plaster (blue-grey). Fibrin is a protein formed from the conversion of clotting factors in the blood; the fibrin fibers trap blood cells and platelets to form a solid clot. This not only prevents further bleeding but also protects the open wound from infection.
description: B0007385. 2011 Wellcome Image Award winner Scanning electron micrograph Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j0kk99n7
title: CIL:38808, Dytiscus marginalis. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy; Rheinberg illumination
Parameter imaged: retardance
Source of contrast: intrinsic birefringence
Visualization methods: visualization of contiguous regions
description: Image B0007757. 2011 Wellcome Image Award winner. Light microscopy Sept 2010 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: This polarized photomicrograph shows the rows of suckers on the foreleg of a male Dytiscus marginalis, commonly known as the great diving beetle - the largest freshwater beetle in the UK. It has a large streamlined body that is dark brown in color, with a yellow abdomen and yellow legs. Great diving beetles spend the majority of their time underwater hunting for food, feeding on other insects, tadpoles and small fish. They also mate underwater, and to aid this, the males have developed plate-like proximal tarsal joints on their front legs, which are covered in suckers. These suckers allow the male to hold onto a female during mating. This image shows a portion of such a joint, detailing part of one of the two larger suckers and five rows of small ones. The vibrant colors were achieved by passing light through colored filters onto the specimen, a technique known as Rheinberg illumination.
DOI: 10.6075/j0pk0f2s
title: CIL:38804, Homo sapiens, epithelial cell. In Cell Image Library

description: Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI)
description: Image B0006855 from 2006 Collection: Medical Photographic Library Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Confocal micrograph of lesions in human cervical epithelium infected with human papilloma virus (HPV16). Early viral proteins (green) bind to and re-orgainse the ketatin filaments (red) towards the edge of the cell. Cell nuclei are stained with Dapi (blue).
DOI: 10.6075/j0qr4w0t
title: CIL:38806, Claviceps, Triticum aestivum, fungal cell. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
description: Image B0007810. 2011 Wellcome Image Award winner. Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: This confocal micrograph shows wheat stigma hairs (blue) infected with ergot fungus (shown in light pink). The stigma hairs are part of the female reproductive organs of the plant. When a pollen grain (released by the male reproductive part) adheres to a stigma, a pollen tube grows and fertilizes the plant's ovule. Ergot is a type of fungus from the genus Claviceps that infects flowering grasses and cereals, including wheat, by mimicking the growth of pollen into the ovary. The fungal hyphae, shown in pink, highlight the path the fungus takes through the stigma hairs to colonise the whole plant flower. The fungus forms a dark, purplish sclerotium (a dense mass of branched hyphae) called an ergot in place of the developing wheat grain. Ergot is highly toxic. If infected plants are eaten it causes ergotism, with symptoms that include spasms, hallucinations, psychosis, itching and gangrene. Ergot poisoning is one of the suggested explanations of bewitchment. In the Middle Ages, monks of the Order of St Anthony were known for treating this condition, and the illness became known as St Anthony's fire.
DOI: 10.6075/j02v2f4m
title: CIL:36812. In Cell Image Library

description: The movie was recorded using Nikon Eclipse Ti epifluorescence microscope equipped with an Andor Clara CCD camera. See T. Sanchez et al. 2011. Cilia-like beating of active microtubule bundles. Science 333:456-459.
description: Isolated microtubules and kinesin spontaneously self-organize in vitro creating assemblies that resemble bundles of cilia that undergo synchronous beating. The major components are fluorescent taxol-stabilized tubulin, biotin-kinesin, and streptavidin. The movie shows tubulin trapped at the air-glass interface of an air bubble that has been confined in a 10-μm-thick chamber. The microtubules nucleate assemblies of active bundles perpendicular to the edge of the bubble, and provide important insights into the mechanism of synchronized ciliary beating observed in vivo.
description: Preparation: in vitro assembly
Item type: intensified CCD (ICCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 647
Data qualification: Processed
DOI: 10.6075/j09s1qbq
title: CIL:37378, damselfly, flight muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.
 
 Technical details:
 Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.
 
 Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199
DOI: 10.6075/j0pg1qcg
title: CIL:37131, Rattus, capillary endothelial cell. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
description: Transmission electron micrograph of rat capillary diaphragm 2h post intravenous administration of ferritin. Ferritin is seen entering plasmalemmal vesicles and into the extracellular space. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X62,000.
DOI: 10.6075/j0p55m4h
title: CIL:37130, Rattus, capillary endothelial cell. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X25,000.
description: Transmission electron micrograph of a rat diaphram capillary 24 hrs post intravenous administration of ferritin. Some ferritin is seen in the intracellular vesicles and extracellular spaces. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
DOI: 10.6075/j080517c
title: CIL:37127, Mus musculus, capillary endothelial cell, fenestrated cell. In Cell Image Library

description: Transmission electron micrograph of mouse capillary intestine fenestrated endothelium with hemoglobin in the lumen lumen. The capillary lumen filled with hemoglobin shows continuities across endothelium. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
description: Reference:
Simeonescu, N., M. Simeonescu, and G. E. Palade. 1975. J. Cell Biol. 64:586-607.

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x14,800
DOI: 10.6075/j0ht2mz4
title: CIL:37186, Rattus, hepatocyte. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Transmission electron micrograph of thin section of rat liver with prominent Golgi and smooth ER associated vesicles. At lower right, the junction between hepatocytes harbors a bile canaliculus. The image illustrates the ethanol-stimulated accumulation of low density lipoprotein (LDL) in Golgi of the liver in rats fed ethanol (equivalent to 1 quart of single malt whisky) by gastric tube. This observation was the impetus for the strategy of purifying Golgi factions by allowing them to rise the surface of dense sucrose columns. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x19,000.
DOI: 10.6075/j0z31x89
title: CIL:37178, Rattus, kidney cell, podocytes. In Cell Image Library

description: Preparation: osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
description: Relevant citations: 
Kanwar, Y.S. and M.G. Farquhar. 1979. J. Cell Biol. 81:137-153.
Farquhar, M.G., S.L. Wisssig and G.E. Palade. 1961 . J. Exp. Med. 113:47-66.

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X16,000.
description: Transmission electron micrograph of rat kidney interdigitating kidney foot processes. The series of images in this group have particular historic and cell biologic importance for determining the role of the glomerular basement membrane in urinary filtration, especially the charge properties and size selectivity in retention of proteins in the circulation. The images here clearly show the glomerular filtration barrier. The study also elucidated the role of the basement membrane in some forms of nephrosis. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0sj1j77
title: CIL:37161, Rattus rattus, epithelial cell. In Cell Image Library

description: Transmission electron micrograph of rat intestinal colon showing apical microvilli. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 22,000X.
DOI: 10.6075/j04j0cs2
title: CIL:37137, Rattus, endothelial cell. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x248,000.
description: Transmission electron micrograph of a clathrin coated pt caveola and transgential cut of a coated vesicle in an endothelial cell. Specimen comes from a capillary in a rat tongue. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0k9365p
title: CIL:37329, epidermal cell. In Cell Image Library

description: The video shows a cytoplast that is adhering to a sub-stratum in its central portion. It is also connected by a retraction fiber to its central cell. The cytoplast is engaging in vigorous protrusive activity around its periphery. The small lamellipodia is undergoing ruffling--extensions, folding back, and re-entry of the the protrusion into the main process of the cell. This demonstrates that an anucleate fragment of a cell has the capacity to nucleate actin, engage in ruffling, and retrograde flows. CIL 37336 and 37337 show that these fragments have the capacity to undergo directional locomotion. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw
DOI: 10.6075/j03n223v
title: CIL:37335, epidermal cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw
description: A more fully differentiated epidermal cell. The nucleus and nucleoli are on the left. The video shows the intracellular movements of lysosomes and mitochondria. The lysosomes are the small refractile spherical objects near the nucleus. The mitochrondria are the more elongate elipsoidal organelles. The mitochrondria are moved along microtubules by microtubule-based transport motors. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.
DOI: 10.6075/j03f4n8t
title: CIL:37237, Cavia porcellus, pancreatic cell. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x46,000.

Additional references:
Palade. G.E. 1955. A small particulate component of the cytoplasm. J. Biochem. Biophys. Cytol. 1:59-68.
Palade. G.E. 1956. The endoplasmic reticulum. J. Biochem. Biophys. Cytol. 2(suppl.):85-98.
description: Early transmission electron micrograph of a thin section of the ribosome-studded rough endoplasmic reticulum of guinea pig pancreas. The ribosomes (small dots) were originally called Palade particles. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0s46qm0
title: CIL:37234, Cavia porcellus, epithelial cell of pancreas. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x88,000.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Early transmission electron micrograph of guinea pig pancreas showing ribosome-studded rough endoplasmic reticulum with secretory granules accumulating in the lumen (cisternae). Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0sq8zqv
title: CIL:37338, Mus musculus, fibroblast. In Cell Image Library

description: Preparation: detergent permeabilized; formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: fluorescence microscopy
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; distribution of epitope
Visualization methods: Fluorescein (FITC); primary antibody plus labeled secondary antibody
Processing history: maximum projection
Data qualification: Processed
description: Promyelocytic leukemia nuclear bodies (PML NBs) are dynamic nuclear organelles. They range from 0.1um to 1um in size and exist in most of mammalian cell types. The functions of PML NBs are indicated by their association with a variety of component proteins that are involved in different cellular process. Here shows PML NBs labeled by monoclonal antibody (PML36) in 3T3 cells. .
description: Cells were rinsed in PBS once and fixed for 15 min in 2% formaldehyde in PBS, pH 7.4. After being washed in PBS for 10 min x3, cells were permeablized 5 min in PBS with 0.2% Triton X-100 and 1% normal goat serum. After being washed in PBS plus 1% goat serum for 10 min x3, the following primary antibody (PML36) were added for 1 h at RT. Cells were rinsed in PBS with 1% normal goat serum for 10 min x3, and secondary FITC anti-mouse antibodies were added for 1 h at RT. Cells were rinsed in PBS for 10 min x3 and were observed on a modified Zeiss Axiovert 200M microscope with a plan-neofluar ×40 objective.
The microscope was fitted with dual excitation and emission filter wheels and a Photometrics Coolsnap-HQ camera.
DOI: 10.6075/j0hd7t9v
title: CIL:37337, epidermal cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw
description: An anucleate cytoplast--a piece of lamellipodium detached from its parent cell--is able to migrate autonomously, without a nucleus, Golgi Apparatus, or microtubules. Image made available thru Mark Cooper and Zebrafish-The Living Laboratory.