DOI: 10.5061/dryad.prr4xgxx5
title: Data from: Potential interaction between clorsulon and ivermectin for malaria vector control

description: Mass ivermectin (IVM) treatment of livestock (MITL) is under consideration
 as a malaria control tool as IVM-treated livestock are lethal to
 blood-feeding Anopheles mosquitoes. MITL is routinely used as a
 prophylaxis in livestock to reduce the burden and transmission of helminth
 infections. Recently, there has been a shift in the veterinary IVM market
 in Southeast Asia wherein nearly all standard IVM formulations are now
 co-formulated with clorsulon (CLO). CLO is used to treat the trematode
 liver fluke, Fasciola hepatica. Thus, the co-administration of IVM and CLO
 simultaneously targets multiple livestock infections. Additionally, F.
 hepatica frequently afflicts human populations in endemic areas, making
 control of F. hepatica in livestock beneficial for One Health. CLO
 interrupts glycolysis in F. hepatica, but its potential effect against
 Anopheles mosquitoes has never been evaluated. Anopheles dirus mosquitoes
 were blood-fed CLO across a range of concentrations (1 – 10,000 nM; 0.38 -
 3,807 ng/ml)) and mosquito survival was monitored for ten days. Co-feeding
 experiments were also performed with An. dirus blood-fed on IVM at two
 concentrations (4 and 6 ng/ml) without and with CLO corresponding to peak
 concentration in cattle (Cmax) (2,700 ng/ml) and five times the Cmax
 (13,500 ng/ml), and mosquito survival was monitored for ten days. CLO had
 no mosquito-lethal effect on An. dirus. The IVM and CLO co-feed experiment
 did not indicate any altered effect of IVM on mosquito survival when
 co-fed with CLO. IVM-CLO livestock co-formulations would not likely alter
 the Anopheles mosquito-lethal effect of MITL. The use of MITL-CLO for
 malaria control would have health benefits for livestock, treating
 helminth and liver fluke infections, and additional One Health benefits by
 reducing transmission risk of liver flukes to humans.
description: Direct observation of mosquito mortality, data captured on
 written forms, transcribed to Excel files
description: # Hongsuwong et al. 2024 - Clorsulon Data (CLO and IVM-CLO)
 [https://doi.org/10.5061/dryad.prr4xgxx5](https://doi.org/10.5061/dryad.prr4xgxx5) ## Description of the data and file structure Anopheles dirus mosquitoes were blood-fed various concentrations of clorsulon (sheet 1) or ivermectin plus clorsulon (sheet 2). Mosquito mortality was observed daily for 10 days after the blood meal. When a mosquito died it was removed from the study and recorded. Any mosquitoes alive on day 10 were counted as alive. ### Files and variables #### File: Hongsuwong\_et\_al.*2024*-*Clorsulon\_Data*(CLO\_and\_IVM-CLO).xlsx ##### Variables * mosquito_species = species of Anopheles mosquito evaluated; * Date_of_feed = date mosquito membrane feed was performed; * Rep = replicate number; * Clorsulon.Conc.nM = concentration of clorsulon (nM) fed to mosquitoes; * Clorsulon.Conc.ng.ml. = concentration of clorsulon (ng/mL) fed to mosquitoes; * Ivermectin.Conc.ng.ml. = concentration of ivermectin (ng/mL) fed to mosquitoes; * Day = day of observation post blood-fed that Event occurred (1-10); * Event = death (1) or alive (0) ## Code/software Excel, .xlsx or .csv ## Access information Other publicly accessible locations of the data: * none Data was derived from the following sources: * observational study