cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4822102.v1
title: AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint

description: Faithful chromosome segregation during mitosis relies on a proofreading mechanism that monitors proper kinetochore-microtubule attachments. The spindle assembly checkpoint (SAC) is based on the concerted action of numerous components that maintain a repressive signal inhibiting transition into anaphase until all chromosomes are attached. Here we show that A-Kinase Anchoring Protein 95 (AKAP95) is necessary for proper SAC function. AKAP95-depleted HeLa cells show micronuclei formed from lagging chromosomes at mitosis. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKAP95-dependent enrichment of TPR in the spindle microtubule area in metaphase, then later in the spindle midzone area. AKAP95-depleted cells display faster prometaphase to anaphase transition, escape from nocodazole-induced mitotic arrest and show a partial delocalization from kinetochores of the SAC component MAD1. Our results demonstrate an involvement of AKAP95 in proper SAC function likely through its interaction with TPR.
DOI: 10.6084/m9.figshare.4822030
title: Wheat fermented

description: Images of staches isolated from whole wheat (Triticum aestivum) seeds fermented in water with yeast for four days.<br>
DOI: 10.6084/m9.figshare.4822030.v1
title: Wheat fermented

description: Images of staches isolated from whole wheat (Triticum aestivum) seeds fermented in water with yeast for four days.<br>
DOI: 10.6084/m9.figshare.4822009
title: Sorghum ground boiled

description: Images of starches isolated from ground sorghum (Sorghum bicolor) seeds that were then boiled in water for various lengths of time.<br>
DOI: 10.6084/m9.figshare.4822009.v1
title: Sorghum ground boiled

description: Images of starches isolated from ground sorghum (Sorghum bicolor) seeds that were then boiled in water for various lengths of time.<br>
DOI: 10.6084/m9.figshare.4818790
title: Shorter lifespan, but greater body size and reproductive investment in agrosystem than in natural habitat in a toad

description: <b>Data for the manuscript "Shorter lifespan, but greater body size and reproductive investment in agrosystem than in natural habitat in a toad". </b> <b></b>
DOI: 10.6084/m9.figshare.4818784
title: Nome_BusP.csv

description: All BusP data from ACEP portal
DOI: 10.6084/m9.figshare.4818784.v1
title: Nome_BusP.csv

description: All BusP data from ACEP portal
DOI: 10.6084/m9.figshare.4818790.v1
title: Shorter lifespan, but greater body size and reproductive investment in agrosystem than in natural habitat in a toad

description: <b>Data for the manuscript "Shorter lifespan, but greater body size and reproductive investment in agrosystem than in natural habitat in a toad". </b> <b></b>
DOI: 10.6084/m9.figshare.4818643.v2
title: Coinage in Norway during the King Olav I Tryggvasonʼs reign (995 – 1000) / Возникновение денежного дела в Норвегии в эпоху викингов: пеннинги Улава I (995 – 1000)

description: The article is about a decisive moment in the history of Norway and Russia – the Viking Age – and the very begynning of coinage in Norway. <br>For the first time in Russian numismatic historiography a paper has got all silver pennings minted during the reign of Viking King Olav I Tryggvason, who is equality venerated in Norway and Russia. The author believes that Norwegian imitations of the Anglo-Saxon silver penny “Crux” were hammered in Nidaros (Trondheim) by local silversmiths. Scandinavia had a long tradition of minting imitative coins, such as bracteates, which were used as jewellery. <br>There is reason to assume that the begynning of Vikings’ imitative “Crux” coinage in Norway happened in 997 – 998: Olav I Tryggvason had chosen Nidaros as a royal residence in 997, imitations of the new Anglo-Saxon penny “Long Cross” appeared in Scandinavia in 998 making “Crux” obsolete.<br><i>Copyright following the coin catalogue number (1, 2, 3, 4)</i><br><b>Первая монета</b>: ©2015 Nationalmuseet av Danmark, København, фотограф Т.Б. Кристенсен / Christensen, Tine Bonde (лицензия CC BY-SA 2.5 DK). За отснятую монету признателен Tine Bonde Christensen, museumsinspektør.<br><b>Вторая монета</b>: ©2015 Historiska Museet, Lunds Universitet, Sverige, за предоставление изображения монеты и разрешение на ее публикацию признателен Gitte Ingvardson, antikvarie i Nordisk Numismatik ved Lunds Universitets Historiska Museet. All Rights Reserved.<br><b>Третья монета</b> из Kungliga Myntkabinettet (Stockholm): ©2015 Kulturhistorisk museum, Universitetet i Oslo, фотограф Л.-А. Чепстов-Лусти / Chepstow-Lusty, Lill-Ann (лицензия CC BY-SA 4.0)<br><b>Четвертая монета</b>: ©2015 Münzkabinett, Staatliche Museen zu Berlin (лицензия CC BY-NC-SA 3.0 DE)
DOI: 10.6084/m9.figshare.4818511
title: raw_data.csv

description: Early treatment with natlizumab in RRM slows brain atrophy
DOI: 10.6084/m9.figshare.4818511.v1
title: raw_data.csv

description: Early treatment with natlizumab in RRM slows brain atrophy
DOI: 10.6084/m9.figshare.4818400
title: Supplementary Material for: Long-Term Follow-Up of Children after Venom Immunotherapy: Low Adherence to Anaphylaxis Guidelines

description: <b><i>Background:</i></b> Data on the long-term outcome of children after specific venom immunotherapy (VIT) are limited. Therefore, we assessed sting recurrence and anaphylaxis relapse rates as well as adherence to anaphylaxis guidelines with regard to the availability of emergency equipment and education status. <b><i>Methods:</i></b> For this long-term survey, data of 311 children with a history of anaphylactic reactions to hymenoptera stings were collected by chart review. We included patients who were treated with a 3-year VIT between 1993 and 2009 and had completed a questionnaire. <b><i>Results:</i></b> Forty of the 311 patients were included. Mean VIT duration was 3.1 years. Of the 40 patients included, 29 children (72.5%) received VIT with vespid venom, 9 with bee venom, and 2 patients with both venoms. During a mean follow-up period of 13 years, 20/40 patients (50%) suffered re-stings. Six of the 20 (30%) patients developed again anaphylactic symptoms (grade 1 <i>n</i> = 5, grade 3 <i>n</i> = 1); 2 were allergic to vespid and 4 to bee venom. Of the entire cohort, only 5/40 (12.5%) had appropriate emergency kits according to the guidelines of the European Academy of Allergy and Clinical Immunology. Among the patients who had emergency kits available, one third (5/15) felt uncertain about the correct application of the medication. Less than two thirds of our population (25/40) affirmed that they have been educated in emergency management. The vast majority (95%; 38/40) of our patients did not have allergy follow-ups after VIT completion. <b><i>Conclusions:</i></b> Anaphylactic relapses are not uncommon, and there are considerable deficits in the emergency management of patients. Hence, comprehensive standardized anaphylaxis education programs as well as regular follow-ups of the allergy status are crucial.
DOI: 10.6084/m9.figshare.4818442
title: Depository

description: Data Manuscript
DOI: 10.6084/m9.figshare.4818397
title: Rat somatosensory cortex electrophysiology [Shew Lab]

description: This data was analyzed to produce the results in the following paper published in PLOS One.<br>State-dependent Intrinsic Predictability of Cortical Network Dynamicsby Leila Fakhraei, Shree Hari Gautam, Woodrow L. Shew
DOI: 10.6084/m9.figshare.4818394
title: Mt. Etna volcano high-resolution topography: airborne LiDAR modelling validated by GPS data

description: High-resolution digital topography is essential for land management and planning in any type of territory as well as the reproduction of the Earth surface in a geocoded digital format that allows several Digital Earth applications. In a volcanic environment, Digital Elevation Models are a valid reference for multi-temporal analyses aimed to observe frequent changes of a volcano edifice and for the relative detailed morphological and structural analyses. For the first time, a DTM (Digital Terrain Model) and a DSM (Digital Surface Model) covering the entire Mt. Etna volcano (Italy) derived from the same airborne Light Detection and Ranging (LiDAR) are here presented. More than 250 million 3D LiDAR points have been processed to distinguish ground elements from natural and anthropic features. The end product is the highly accurate representation of Mt. Etna landscape (DSM) and ground topography (DTM) dated 2005. Both models have a high spatial resolution of 2 m and cover an area of 620 km<sup>2</sup>. The DTM has been validated by GPS ground control points. The vertical accuracy has been evaluated, resulting in a root-mean-square-error of ± 0.24 m. The DTM is available as electronic supplement and represents a valid support for various scientific studies.
DOI: 10.6084/m9.figshare.4818442.v2
title: Depository

description: Data Manuscript
DOI: 10.6084/m9.figshare.4818385
title: Dataset for: Sodium glucose transporter-2 inhibition has no renoprotective effects in non-diabetic chronic kidney disease induced by hyperoxaluria

description: Sodium glucose transporter (SGLT)-2 inhibition has renoprotective effects in diabetic kidney disease. Whether similar effects can be achieved also in non-diabetic kidney disease is speculative. Chronic kidney disease was induced in C57BL/6N mice by feeding an oxalate-rich diet for 14 days, known to induce nephrocalcinosis-related tubular atrophy and interstitial fibrosis without directly affecting the glomerular compartment. Empagliflozin treatment started from day 0 of oxalate feeding had no effect on the decline of glomerular filtration rate, crystal deposition, blood urea nitrogen or serum creatinine levels on day 7 and 14. Tissue morphometry of tubular injury and kidney mRNA levels of kidney injury molecule-1 or tissue inhibitor of metalloproteinase-2 were comparable between empagliflozin- and vehicle-treated mice with oxalate nephropathy on day 7 and 14. Similarly, empagliflozin did not affect markers of interstitial fibrosis, including silver, alpha smooth muscle actin (αSMA) and collagen 1 staining, and mRNA levels of fibronectin-1, collagen 1α1, fibroblast-specific protein-1, and transforming growth factor (TGF)-β2 on day 7 and 14. Thus, the specific renoprotective mechanisms-of-action of SGLT2 inhibition in diabetic kidney disease do not apply to chronic oxalosis, a non-diabetic form of chronic kidney disease.
DOI: 10.6084/m9.figshare.4818442.v1
title: Depository

description: Data Manuscript
DOI: 10.6084/m9.figshare.4818382
title: Dataset for: Dopaminergic activation anticipates daily nursing in the rabbit

description: Maternal care is a motivated behavior and in the rabbit it is restricted to the spontaneous return of the mother to nurse her pups for just a few minutes once a day. Previously we have reported neural activation of brain areas and neuroendocrine cells after nursing. However, this daily spontaneous return suggests that the mother is in a high motivational state to nurse her pups. Here we hypothesized that during anticipation of nursing there is an activation of dopaminergic neurons of the mesolimbic system and in their target areas. Then we explored, by the expression of FOS protein, possible activation of the mesolimbic system as well as dopaminergic cells of the A10 cell group before and after nursing and in control does. Additionally, we measured FOS expression in the preoptic area and lateral septum. We found a significant increase of FOS before nursing, and a further increase after nursing, in the mesolimbic system and dopaminergic cells as well as in the preoptic area and lateral septum. Interestingly, the medial prefrontal area shows an intense activation during anticipation of nursing, which remains after nursing. We conclude that the activation of the mesolimbic system before nursing is related to the high locomotor behavior prior to the next nursing bout and support the proposal that the mother is in a high motivational state at the time of returning to the nest. The additional activation after nursing can be related to the neuroendocrine and neural consequences of the milk ejection reflex by suckling.
DOI: 10.6084/m9.figshare.4818385.v1
title: Dataset for: Sodium glucose transporter-2 inhibition has no renoprotective effects in non-diabetic chronic kidney disease induced by hyperoxaluria

description: Sodium glucose transporter (SGLT)-2 inhibition has renoprotective effects in diabetic kidney disease. Whether similar effects can be achieved also in non-diabetic kidney disease is speculative. Chronic kidney disease was induced in C57BL/6N mice by feeding an oxalate-rich diet for 14 days, known to induce nephrocalcinosis-related tubular atrophy and interstitial fibrosis without directly affecting the glomerular compartment. Empagliflozin treatment started from day 0 of oxalate feeding had no effect on the decline of glomerular filtration rate, crystal deposition, blood urea nitrogen or serum creatinine levels on day 7 and 14. Tissue morphometry of tubular injury and kidney mRNA levels of kidney injury molecule-1 or tissue inhibitor of metalloproteinase-2 were comparable between empagliflozin- and vehicle-treated mice with oxalate nephropathy on day 7 and 14. Similarly, empagliflozin did not affect markers of interstitial fibrosis, including silver, alpha smooth muscle actin (αSMA) and collagen 1 staining, and mRNA levels of fibronectin-1, collagen 1α1, fibroblast-specific protein-1, and transforming growth factor (TGF)-β2 on day 7 and 14. Thus, the specific renoprotective mechanisms-of-action of SGLT2 inhibition in diabetic kidney disease do not apply to chronic oxalosis, a non-diabetic form of chronic kidney disease.
DOI: 10.6084/m9.figshare.4818379
title: Dataset for: Ventriculo-Arterial Coupling Detects Occult RV Dysfunction in Chronic Thromboembolic Pulmonary Vascular Disease

description: Chronic thromboembolic disease (CTED) is sub-optimally defined by a mean pulmonary artery pressure (mPAP) &lt; 25mmHg at rest in patients that remain symptomatic from chronic pulmonary artery thrombi. To improve identification of RV pathology in patients with thromboembolic obstruction, we hypothesized that the right ventricular (RV) ventriculo-arterial (Ees/Ea) coupling ratio at maximal stroke work (Ees/Eamax sw) derived from an animal model of pulmonary obstruction may be used to identify occult RV dysfunction (low Ees/Ea) or residual RV energetic reserve (high Ees/Ea). Eighteen open chested pigs had conductance catheter RV pressure-volume (PV)-loops recorded during PA snare to determine Ees/Eamax sw. This was then applied to ten patients with chronic thromboembolic pulmonary hypertension (CTEPH) and ten patients with CTED, also assessed by RV conductance catheter and cardiopulmonary exercise testing. All patients were then re-stratified by Ees/Ea. The animal model determined an Ees/Eamax sw = 0.68±0.23 threshold, either side of which cardiac output and RV stroke work fell. Two patients with CTED were identified with an Ees/Ea well below 0.68 suggesting occult RV dysfunction whilst three patients with CTEPH demonstrated Ees/Ea ≥ 0.68 suggesting residual RV energetic reserve. Ees/Ea &gt; 0.68 and Ees/Ea &lt; 0.68 sub-groups demonstrated constant RV stroke work but lower stroke volume (87.7±22.1 vs. 60.1±16.3mL respectively, p=0.006) and higher end-systolic pressure (36.7±11.6 vs. 68.1±16.7mmHg respectively, p&lt;0.001). Lower Ees/Ea in CTED also correlated with reduced exercise ventilatory efficiency. Low Ees/Ea aligns with features of RV maladaptation in CTED both at rest and on exercise. Characterisation of Ees/Ea in CTED may allow for better identification of occult RV dysfunction.
DOI: 10.6084/m9.figshare.4818370
title: Dataset for: Blindfolding during wakefulness causes decrease in sleep slow wave activity

description: Slow wave activity (SWA, 0.5-4 Hz) represents the predominant EEG oscillatory activity during slow wave sleep (SWS). Its amplitude is considered in part a reflection of synaptic potentiation in cortical networks due to encoding of information during prior waking, with higher amplitude indicating stronger potentiation. Previous studies showed that increasing and diminishing specific motor behaviours produced corresponding changes in SWA in the respective motor cortical areas during subsequent SWS. Here, we tested whether this relationship can be generalized to the visual system, i.e., whether diminishing encoding of visual information likewise leads to a localized decrease in SWA over the visual cortex. Experiments were performed in healthy men whose eyes on two different days were or were not covered for 10.5 h before bedtime. The subject’s EEG was recorded during sleep and, after sleep, visual evoked potentials (VEPs) were recorded. SWA during non-rapid eye movement sleep (NonREM sleep) was lower after blindfolding than after eyes open (P &lt; 0.01). The decrease in SWA that was most consistent during the first 20 min of NonREM sleep, did not remain restricted to visual cortex regions, with changes over frontal and parietal cortical regions being even more pronounced. In the morning after sleep, the N75-P100 peak-to-peak-amplitude of the VEP was significantly diminished in the blindfolded condition. Our findings confirm a link between reduced wake encoding and diminished SWA during ensuing NonREM sleep, although this link appears not to be restricted to sensory cortical areas.
DOI: 10.6084/m9.figshare.4818373
title: Dataset for: Light-dependent expression of a Na+/H+ exchanger 3-like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light-enhanced calcification

description: Na+/H+ exchangers (NHEs) regulate intracellular pH and ionic balance by mediating H+ efflux in exchange for Na+ uptake in a 1:1 stoichiometry. This study aimed to obtain from the ctenidium of the giant clam Tridacna squamosa (TS) the complete cDNA sequence of a NHE3-like transporter (TSNHE3), and to determine the effect of light exposure on its mRNA expression level and protein abundance therein. The coding sequence of TSNHE3 comprised 2886 bp, encoding 961 amino acids with an estimated molecular mass of 105.7 kDa. Immunofluorescence microscopy revealed that TSNHE3 was localized to the apical membrane of epithelial cells of the ctenidial filaments and the tertiary water channels. Particularly, the apical immunofluorescence of the ctenidial filaments was consistently stronger in the ctenidium of clams exposed to 12 h of light than those of the control kept in darkness. Indeed, light induced significant increases in the transcript level and protein abundance of TSNHE3/TSNHE3 in the ctenidium, indicating that the transcription and translation of TSNHE3/TSNHE3 were light-dependent. As light-enhanced calcification generates H+, the increased expression of TSNHE3/TSNHE3 in the ctenidium could be a response to augment H+ excretion in pursuance of whole-body acid-base balance during light exposure. These results signify that shell formation in giant clams requires the collaboration between the ctenidium, which is a respiratory and iono-regulatory organ, and the inner mantle, which is directly involved in the calcification process, and provide new insights into the mechanisms of light-enhanced calcification in giant clams.
DOI: 10.6084/m9.figshare.4818376
title: Dataset for: NF-kappaB signalling is involved in immune-modulation, but not basal functioning, of the mouse suprachiasmatic circadian clock.

description: Circadian rhythms are recurring near-twenty four hour patterns driven by an endogenous circadian timekeeping system. The master pacemaker in this system is the hypothalamuc suprachiasmatic nucleus (SCN) . Recently interest has been drawn to how the SCN clock responds to immune system stimulation. A major signalling component in the immune system is nuclear factor (NF)-kappaB. In the present study we examined the role of NF-kappaB in SCN function. Whilst serum shocked fibroblasts showed rhythmic nuclear localisation of p65 and p65-dependent transcription, there were no circadian changes in the SCN in expression of the NF-kappaB components p65, c-Rel, p-IkappaB or p-IKK. Chronic treatment with the NF-kappaB inhibitor PDTC did not impact on circadian or diurnal rhythms. Phase-shifting light pulses did not impact on SCN expression of p65, and PDTC treatment did not attenuate the behavioural or molecular response to light pulses. Peripheral treatment with lipopolysaccharide resulted in increased NF-kappaB component expression in the SCN. In vitro experiments with SCN slice cultures showed that treatment with NF-kappaB inhibitors did not markedly alter rhythmic changes in PER2::LUC expression. Further, SCN slices from nf-kappab::luc mice did not show any evidence for circadian rhythms in NF-kappaB-mediated transcription. Experiments utilising older mice (~16 months old) showed that SCN treatment in vitro with PDTC resulted in increased amplitude of rhythmic PER2::LUC expression, and LPS treatment resulted in altered PER2::LUC rhythm acrophase. Overall, we interpret our results as providing evidence for the involvement of NF-kappaB in the suprachiasmatic circadian clock following immune stimulation, but not under basal conditions.
DOI: 10.6084/m9.figshare.4818379.v1
title: Dataset for: Ventriculo-Arterial Coupling Detects Occult RV Dysfunction in Chronic Thromboembolic Pulmonary Vascular Disease

description: Chronic thromboembolic disease (CTED) is sub-optimally defined by a mean pulmonary artery pressure (mPAP) &lt; 25mmHg at rest in patients that remain symptomatic from chronic pulmonary artery thrombi. To improve identification of RV pathology in patients with thromboembolic obstruction, we hypothesized that the right ventricular (RV) ventriculo-arterial (Ees/Ea) coupling ratio at maximal stroke work (Ees/Eamax sw) derived from an animal model of pulmonary obstruction may be used to identify occult RV dysfunction (low Ees/Ea) or residual RV energetic reserve (high Ees/Ea). Eighteen open chested pigs had conductance catheter RV pressure-volume (PV)-loops recorded during PA snare to determine Ees/Eamax sw. This was then applied to ten patients with chronic thromboembolic pulmonary hypertension (CTEPH) and ten patients with CTED, also assessed by RV conductance catheter and cardiopulmonary exercise testing. All patients were then re-stratified by Ees/Ea. The animal model determined an Ees/Eamax sw = 0.68±0.23 threshold, either side of which cardiac output and RV stroke work fell. Two patients with CTED were identified with an Ees/Ea well below 0.68 suggesting occult RV dysfunction whilst three patients with CTEPH demonstrated Ees/Ea ≥ 0.68 suggesting residual RV energetic reserve. Ees/Ea &gt; 0.68 and Ees/Ea &lt; 0.68 sub-groups demonstrated constant RV stroke work but lower stroke volume (87.7±22.1 vs. 60.1±16.3mL respectively, p=0.006) and higher end-systolic pressure (36.7±11.6 vs. 68.1±16.7mmHg respectively, p&lt;0.001). Lower Ees/Ea in CTED also correlated with reduced exercise ventilatory efficiency. Low Ees/Ea aligns with features of RV maladaptation in CTED both at rest and on exercise. Characterisation of Ees/Ea in CTED may allow for better identification of occult RV dysfunction.