cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.17645512
title: Helen Keller Method for Legal Learning (Version 1) 海伦凯特法用来为10-17岁学生学习法律

description:  





This file outlines an embodied learning pathway inspired by Helen Keller's tactile language acquisition.

It describes how tactile patterns, rhythmic gestures, object-touch mapping, and embodied contrast can be used to teach legal concepts, reasoning, and multi-lingual expression to youth.

The method forms one of the three foundations of the Tactile Jurisprudence System.

 

 

 

Keywords: tactile learning, Helen Keller, embodied cognition, legal reasoning, multi-sensory learning
DOI: 10.15468/rujqnw
title: Suivi des oiseaux de passage : programme ACT - Programme ACT : Répartition Grive littorne 1998 - 2002 Coordonnées GPS Lambert 93

description: Aire de reproduction de l'espèce Grive littorne fondée sur la présence de mâles chanteurs au printemps (avril – juin), à partir de points d’écoutes répartis le long d’itinéraires échantillons. Environ 5 000 points ont été visités chaque année entre 1998 et 2002
DOI: 10.15468/dl.q7mzbt
title: Occurrence Download

description: A dataset containing 2732 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Araucaria angustifolia)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 2732 records from 127 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046603-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fabpmn
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Kryptopterus geminus)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 21 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046604-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34934/dvn/c0dgss
title: Seed lists for BelgicaWeb research project related to the archiving of web and social media content

description: These spreadsheets are part of the research data produced within the BelgicaWeb project. It is a BRAIN 2.0 project funded by BELSPO (2024-2026). BelgicaWeb aims to make Belgium’s born-digital heritage accessible and FAIR, i.e. Findable, Accessible, Interoperable and Reusable by developing a user-friendly access platform and an API that enables access at data level. The spreadsheets list the seeds that were given to the Browsertrix crawler software to create a corpus of archived web and social media content.
DOI: 10.48420/29986198
title: Anonymised ELOM 4& 5 SASL data set of 29 deaf children.

description: This is an anonymised data set of the performance of 29 deaf children who completed the ELOM 4 & 5 in South African Sign Language recorded by each of the 5 domains of performance, not by single item. It is in EXCEL format.
DOI: 10.48420/29986198.v1
title: Anonymised ELOM 4& 5 SASL data set of 29 deaf children.

description: This is an anonymised data set of the performance of 29 deaf children who completed the ELOM 4 & 5 in South African Sign Language recorded by each of the 5 domains of performance, not by single item. It is in EXCEL format.
DOI: 10.15468/dl.egak8m
title: Occurrence Download

description: A dataset containing 369 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "OccurrenceStatus is Present",
 "TaxonKey is Hieraaetus pennatus",
 "Year 2020-2025"
 ]
}

 The dataset includes 369 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046605-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5519/qd.jc4j6qbo
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (1497 records)
DOI: 10.22002/scm3m-2tw14
title: Halobacterium salinarum

description: Archaella and plate
description: <strong>Tilt Series Date:</strong> 2017-05-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Halobacterium salinarum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard H. salinarum medium</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2017-05-26-7_slicer18112.jpg: polar cap
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-7/rawdata/</p>Halobacterium_20170525c0002.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-7/rawdata/Halobacterium_20170525c0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        Halobacterium_20170525c0002_part61_13.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-7/3dimage_84760/Halobacterium_20170525c0002_part61_13.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-05-26-7_slicer18103.png,  Gas vesicle, 1.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-7_slicer18103.png"
        > <i class="download icon"></i> Download </a></p>

        dga2017-05-26-7_slicer18104.png,  Gas vesicle, 1.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-7_slicer18104.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zewbg-f4q14
title: Xanthomonas vs Caulobac (-T1SS)

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter (delta-cdzAB) competition. Caulobacter strain is a T1SS deletion strain.
description: <strong>Tilt Series Date:</strong> 2017-10-21</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the Xac strain, Caulobacter strain is from Laub lab. Data collected and processed by Mohammed Kaplan and Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-21-33/rawdata/</p>Xan_CaulDelT1_20172110b0008.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-21-33/rawdata/Xan_CaulDelT1_20172110b0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172110b0008_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-21-33/3dimage_108752/Xan_CaulDelT1_20172110b0008_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172110b0008.tlt,  336.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-21-33/file_25664/Xan_CaulDelT1_20172110b0008.tlt"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172110b0008_fid.tlt,  336.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-21-33/file_25665/Xan_CaulDelT1_20172110b0008_fid.tlt"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ha7x4-qj062
title: bapta_scale7

description: Raw data files of bapta_scale7
description: <strong>Tilt Series Date:</strong> 2020-11-09</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Allium cepa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing</p>
<strong>Sample Preparation:</strong> Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-11-09-3/rawdata/</p>on25-3_lam1_ts3.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-11-09-3/rawdata/on25-3_lam1_ts3.mrc"
        > <i class="download icon"></i> Download </a></p>

        on25-3_lam1_ts3_rec.hdf,  Reconstruction (Pixel Size 1.32 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-11-09-3/3dimage_141686/on25-3_lam1_ts3_rec.hdf"
        > <i class="download icon"></i> Download </a></p>

        on25-3_lam1_ts3.rawtlt,  272.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-11-09-3/file_29895/on25-3_lam1_ts3.rawtlt"
        > <i class="download icon"></i> Download </a></p>

        on25-3_lam1_ts3.mrc.mdoc,  19.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-11-09-3/file_29896/on25-3_lam1_ts3.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tqbkg-hwf85
title: non_treated_scale8

description: Raw data files of non_treated_scale8
description: <strong>Tilt Series Date:</strong> 2020-02-12</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Allium cepa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing</p>
<strong>Sample Preparation:</strong> Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-12/rawdata/</p>on17-1_lam2_ts5.st,  Tilt Series (Pixel Size 0.33 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-12/rawdata/on17-1_lam2_ts5.st"
        > <i class="download icon"></i> Download </a></p>

        on17-1_lam2_ts5.rec,  Reconstruction (Pixel Size 1.32 nm), 451.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-12/3dimage_141673/on17-1_lam2_ts5.rec"
        > <i class="download icon"></i> Download </a></p>

        on17-1_lam2_ts5.rawtlt,  272.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-12/file_29883/on17-1_lam2_ts5.rawtlt"
        > <i class="download icon"></i> Download </a></p>

        on17-1_lam2_ts5.mrc.mdoc,  19.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-12/file_29884/on17-1_lam2_ts5.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tfztf-wn431
title: non_treated_scale6

description: Raw data files of non_treated_scale6
description: <strong>Tilt Series Date:</strong> 2019-11-13</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Allium cepa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong>  Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing</p>
<strong>Sample Preparation:</strong> Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-3/rawdata/</p>on14-3_lam1_ts5.st,  Tilt Series (Pixel Size 0.33 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-3/rawdata/on14-3_lam1_ts5.st"
        > <i class="download icon"></i> Download </a></p>

        on14-3_lam1_ts5.rec,  Reconstruction (Pixel Size 1.32 nm), 513.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-3/3dimage_141657/on14-3_lam1_ts5.rec"
        > <i class="download icon"></i> Download </a></p>

        on14-3_lam1_ts5.rawtlt,  264.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-3/file_29867/on14-3_lam1_ts5.rawtlt"
        > <i class="download icon"></i> Download </a></p>

        on14-3_lam1_ts5.mrc.mdoc,  18.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-3/file_29868/on14-3_lam1_ts5.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ka3d8j
title: Occurrence Download

description: A dataset containing 2806 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pinus koraiensis"
 ]
}

 The dataset includes 2806 records from 190 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046606-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yrtbe7
title: Occurrence Download

description: A dataset containing 1735 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acer negundo",
 "Country is Austria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1735 records from 35 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046609-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/83n8r-nqh47
title: non_treated_scale8

description: Raw data files of non_treated_scale8
description: <strong>Tilt Series Date:</strong> 2019-11-13</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Allium cepa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong>  Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing</p>
<strong>Sample Preparation:</strong> Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-9/rawdata/</p>on17-1_lam2_ts3.st,  Tilt Series (Pixel Size 0.32 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-9/rawdata/on17-1_lam2_ts3.st"
        > <i class="download icon"></i> Download </a></p>

        on17-1_lam2_ts3.rec,  Reconstruction (Pixel Size 1.32 nm), 890.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-9/3dimage_141669/on17-1_lam2_ts3.rec"
        > <i class="download icon"></i> Download </a></p>

        on17-1_lam2_ts3.rawtlt,  248.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-9/file_29879/on17-1_lam2_ts3.rawtlt"
        > <i class="download icon"></i> Download </a></p>

        on17-1_lam2_ts3.mrc.mdoc,  17.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-11-13-9/file_29880/on17-1_lam2_ts3.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a04b3-va805
title: non_treated_scale5

description: Raw data files of non_treated_scale5
description: <strong>Tilt Series Date:</strong> 2020-02-12</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Allium cepa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong>  Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing</p>
<strong>Sample Preparation:</strong> Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-6/rawdata/</p>on15-4_lam2_ts4.st,  Tilt Series (Pixel Size 0.33 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-6/rawdata/on15-4_lam2_ts4.st"
        > <i class="download icon"></i> Download </a></p>

        on15-4_lam2_ts4.rec,  Reconstruction (Pixel Size 1.32 nm), 816.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-6/3dimage_141643/on15-4_lam2_ts4.rec"
        > <i class="download icon"></i> Download </a></p>

        on15-4_lam2_ts4.rawtlt,  296.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-6/file_29853/on15-4_lam2_ts4.rawtlt"
        > <i class="download icon"></i> Download </a></p>

        on15-4_lam2_ts4.mrc.mdoc,  20.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-6/file_29854/on15-4_lam2_ts4.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>