cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1465034.v1
title: Data for Carter & Leffer. Social grooming in bats: are vampire bats exceptional? PLOS One.

description: All bats were cared for by the Organization for Bat Conservation at the Cranbrook Institute of Science, Bloomfield Hills, Michigan (under permits: USDA 34-C-0117; US Fish and Wildlife Service MB003342-0), housed in flight cages that allowed free association, and kept at 25-28 degrees Celsius with &gt;33% humidity on a half-reversed 12 h light/dim light cycle. Sex could be assigned because male Artibeus, Carollia, Eidolon, and Rousettus were housed together, while females and some castrated males (see below) of these species were housed together in a different cage. All Desmodus were housed together, but sex could be assigned with certainty because sexes tended to segregate (into female groups with a dominant male and satellite male groups) and bats were individually marked. In all species, we only observed interactions between adult bats. To compare social grooming and other behaviors across species, we conducted randomized focal sampling. We took instantaneous (“on the beep”) focal samples of a randomly chosen bat. For Artibeus, Desmodus, and Carollia, we obtained 13 samples from both males and females (see Table 1 for numbers of bats). For the other two species, we sampled 13 males and 13 bats that could have been either female (Eidolon n=6, Rousettus n=9) or a castrated male (Eidolon n=2, Rousettus n=2). We refer to this category of female and castrated males as “no testes” bats. Observers chose a focal bat randomly by counting bats left to right until a specific random number was reached. If a focal bat was asleep or became lost from view, the observer immediately began focal sampling the next closest conspecific in the same sex category. During each of 26 focal sampling sessions, observers took one observation every 10 s for 10 min. During each observation, observers reported the presence or absence of social grooming (chewing or licking another bat’s body), self-grooming (scratching or licking its own body), feeding, and aggression. Observers also switched to a different species and sex after each observation, there was no reason to expect biased sampling of sexes or species.
DOI: 10.6084/m9.figshare.1465038.v1
title: Fungal Classification 2015

description: Work in Progress - part of the Open Tree of Life - Classification update - Hibbett et al. 2007
DOI: 10.6084/m9.figshare.1465038.v2
title: Fungal Classification 2015

description: Work in Progress - part of the Open Tree of Life - Classification update - Hibbett et al. 2007
DOI: 10.6084/m9.figshare.1465038.v3
title: Fungal Classification 2015

description: Work in Progress - part of the Open Tree of Life - Classification update - Hibbett et al. 2007
DOI: 10.6084/m9.figshare.1465038.v4
title: Fungal Classification 2015

description: Work in Progress - part of the Open Tree of Life - Classification update - Hibbett et al. 2007
DOI: 10.6084/m9.figshare.1465038.v6
title: Fungal Classification 2015

description: Work in Progress - part of the Open Tree of Life - Classification update - Hibbett et al. 2007
DOI: 10.6084/m9.figshare.1465038.v5
title: Fungal Classification 2015

description: Work in Progress - part of the Open Tree of Life - Classification update - Hibbett et al. 2007
DOI: 10.6084/m9.figshare.1466767.v21
title: modish.pl (2015)

description: Experimental Perl program for BPS software. It brings into account the reflective effect of solar obstructions on solar gains in ESP-r models. It needs Radiance installed.
DOI: 10.6084/m9.figshare.1465025
title: Turiasauria-like teeth from the Upper Jurassic of the Lusitanian Basin, Portugal

description: Turiasauria is a clade of eusauropods with a wide stratigraphic range that could extend from the Bathonian to the lower Aptian including <i>Turiasaurus</i>, <i>Losillasaurus</i>, <i>Zby</i> and putatively, <i>Galveosaurus</i>, <i>Atlasaurus</i> and isolated remains from Middle Jurassic-to-Lower Cretaceous. Some are characterised by the presence of heart-shaped teeth. Several tooth occurrences from the Portuguese Upper Jurassic with this type of morphology (SI: 1.1–1.8) are reported and discussed. If this morphology is regarded as synapomorphic of Turiasauria, the teeth will be tentatively related to this clade. From a sample of 43 teeth, three main morphotypes are described. Three hypotheses might explain the morphological variation: (1) the range of tooth morphologies indicates variation in the jaw, (2) the range of tooth morphologies indicates taxonomic variation or (3) a combination of both. The general wear pattern in morphotypes I and II starts with a distal facet, then the appearance of mesial/apical facet and finally a ‘V’-shaped facet. In morphotype III, the wear begins with a mesial facet. The variability observed for Portuguese Upper Jurassic specimens is congruent with the morphological variability along the tooth row shown by other sauropods with spatulate/spoon-shaped teeth and it is considered the most parsimonious hypothesis to explain it.
DOI: 10.6084/m9.figshare.1465025.v1
title: Turiasauria-like teeth from the Upper Jurassic of the Lusitanian Basin, Portugal

description: Turiasauria is a clade of eusauropods with a wide stratigraphic range that could extend from the Bathonian to the lower Aptian including <i>Turiasaurus</i>, <i>Losillasaurus</i>, <i>Zby</i> and putatively, <i>Galveosaurus</i>, <i>Atlasaurus</i> and isolated remains from Middle Jurassic-to-Lower Cretaceous. Some are characterised by the presence of heart-shaped teeth. Several tooth occurrences from the Portuguese Upper Jurassic with this type of morphology (SI: 1.1–1.8) are reported and discussed. If this morphology is regarded as synapomorphic of Turiasauria, the teeth will be tentatively related to this clade. From a sample of 43 teeth, three main morphotypes are described. Three hypotheses might explain the morphological variation: (1) the range of tooth morphologies indicates variation in the jaw, (2) the range of tooth morphologies indicates taxonomic variation or (3) a combination of both. The general wear pattern in morphotypes I and II starts with a distal facet, then the appearance of mesial/apical facet and finally a ‘V’-shaped facet. In morphotype III, the wear begins with a mesial facet. The variability observed for Portuguese Upper Jurassic specimens is congruent with the morphological variability along the tooth row shown by other sauropods with spatulate/spoon-shaped teeth and it is considered the most parsimonious hypothesis to explain it.
DOI: 10.6084/m9.figshare.1465024
title: Dynamic light scattering studies of L35Ae samples

description: Hydrodynamic diameters for L35Ae samples (1.5 mg/ml) were measured by DLS at 25.0°C. The protein solutions were preliminarily passed through 10 nm filter.Buffer conditions: PBS, 150 mM NaCl, pH 7.0.
DOI: 10.6084/m9.figshare.1466767.v22
title: A tentative procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios.

description: Experimental Perl program for BPS software. It brings into account the reflective effect of solar obstructions on solar gains in ESP-r models. It needs Radiance installed.
DOI: 10.6084/m9.figshare.1465024.v1
title: Dynamic light scattering studies of L35Ae samples

description: Hydrodynamic diameters for L35Ae samples (1.5 mg/ml) were measured by DLS at 25.0°C. The protein solutions were preliminarily passed through 10 nm filter.Buffer conditions: PBS, 150 mM NaCl, pH 7.0.
DOI: 10.6084/m9.figshare.1465020
title: High resolution gel filtration of L35Ae samples

description: Gel-filtration chromatography data for L35Ae samples (3.5-3.7 mg/ml) and molecular mass standards at room temperature (Superdex 75 10/300 GL column). The apparent molecular masses of L35Ae samples were determined by comparison of their elution times with the calibration line.
DOI: 10.6084/m9.figshare.1465021
title: Pyrethroids and nectar toxins have subtle effects on the motor function, grooming and wing fanning behaviour of honeybees (Apis mellifera)

description: Motor function of individual honeybees exposed to pesticides and nectar toxins that activate sodium channels. Data collected as continuous recordings over 10 min intervals.
DOI: 10.6084/m9.figshare.1466767.v23
title: A tentative procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios. (2015)

description: Experimental Perl program for BPS software. It brings into account the reflective effect of solar obstructions on solar gains in ESP-r models. It needs Radiance installed.
DOI: 10.17632/5njfkgdzx4.1
title: Survey data of COVID-19 related Knowledge, Attitude and Practice and the Impact on Dentistry among Nepalese Dentists.

description: To assess the knowledge, awareness, and practice (KAP) of the Dentists to combat the pandemic which could help shape future guidelines and policies to be implemented in dental settings. The cross-sectional descriptive study was conducted solely online with series of multiple-choice questionnaires collecting responses till the determined sample size was reached. The ethical approval for the study was obtained from Nepal Health Research Council. Electronic informed consent was provided on the initial page of the survey. The positive response was considered as implied consent. The NMC registered Dentists who responded to all the questions of the survey were included while incomplete responses were excluded from the survey. An online structured survey composed of 26 questions created using the free-access Google Forms application. Pretesting of the survey instrument was done and refinements were made as required to facilitate better comprehension and to organize the questions before the final survey instrument was formed. The questionnaire was pretested among ten dental surgeons in Bharatpur. Inter responder reliability was tested among five responders and was found to have high reliability. It was sent to dental practitioners with the link to the online survey sent through an anonymous mailing and social media messaging list to currently practicing dentists across the country regardless of their place of work either in private clinics, hospitals, or health centers. Confidentiality was maintained throughout the study by making participants' information anonymous and not asking to enter their personal details at any point. Eligible dentists’ participation in this survey was completely voluntary. Filled pro forma was collected via e-survey and after completion of the assigned sample size of participants; it was recorded in spreadsheet software as a master chart for statistical analysis. Statistical analysis was performed by the primary and secondary authors themselves using SPSS version 20 for mac OS. Descriptive statistics was performed, and frequencies of responses were reported in proportion. Nonparametric tests of independence and Chi-square test was performed to see the association between categorical variables. Around 84% of the respondents accurately answered mode of transmission, 68% inquired about the travel history while only 49% measured the body temperature. Also, only 42% were receiving salary. A statistically significant difference concerning impact and practice during the COVID-19 was observed between general practitioner and specialist working at different workplaces.
DOI: 10.6084/m9.figshare.1465019
title: GuHCl-induced unfolding of L35Ae samples

description: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm and position of tryptophan fluorescence spectrum maximum. Excitation wavelength was 280 nm. Protein concentration was 3 µM. Buffer conditions: PBS, 150 mM NaCl, pH 7.0. Boltzmann function was used for theoretical fits of the experimental data.
DOI: 10.6084/m9.figshare.1465019.v1
title: GuHCl-induced unfolding of L35Ae samples

description: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm and position of tryptophan fluorescence spectrum maximum. Excitation wavelength was 280 nm. Protein concentration was 3 µM. Buffer conditions: PBS, 150 mM NaCl, pH 7.0. Boltzmann function was used for theoretical fits of the experimental data.
DOI: 10.6084/m9.figshare.1466767.v24
title: A tentative procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios. (2015)

description: Experimental Perl program for BPS software. It brings into account the reflective effect of solar obstructions on solar gains in ESP-r models. It needs Radiance installed.
DOI: 10.6084/m9.figshare.1465009
title: MFISH raw data for Sumption etal 2015

description: MFISH cytogenetic raw data comprising work published in Sumption etal PLoS ONE 2015
DOI: 10.6084/m9.figshare.1466767.v25
title: A tentative procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios. (2015)

description: Experimental Perl program for BPS software. It brings into account the reflective effect of solar obstructions on solar gains in ESP-r models. It needs Radiance installed.
DOI: 10.6084/m9.figshare.1465009.v1
title: MFISH raw data for Sumption etal 2015

description: MFISH cytogenetic raw data comprising work published in Sumption etal PLoS ONE 2015
DOI: 10.6084/m9.figshare.1465014.v1
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins, which colocalize with endogenous galectins, renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging and inflammation.
DOI: 10.6084/m9.figshare.1466767.v26
title: Procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios. (2015-16)

description: Experimental program for Building Performance Simulation software. It brings into account the reflective effect of solar obstructions on solar gains in ESP-r building models on the basis of irradiance ratios. Those ratios are obtained combining the direct radiation on a surface and the total radiation calculated by the means of a raytracer (Radiance) on the same surface.
DOI: 10.6084/m9.figshare.1465014.v2
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.6084/m9.figshare.1466767.v27
title: Procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios. (2015-16)

description: Program which brings into account the reflective effect of solar obstructions on solar gains in the ESP-r building models on the basis of irradiance ratios. Those ratios are obtained combining the direct radiation on a surface and the total radiation on it calculated by the means of the Radiance raytracer. Modish can be installed from the command line by typing: sudo cpanm Sim::OPT::Modish
DOI: 10.6084/m9.figshare.1464992
title: Resistance of L35Ae samples towards chemical denaturants

description: GuHCl-induced changes in position of tryptophan fluorescence spectrum maximum of L35Ae. Theoretical fits to the experimental data using Boltzmann function were made. Buffer contained PBS, 150 mM NaCl, pH 7.0. Protein concentration was 3 µM.
DOI: 10.6084/m9.figshare.1465014.v3
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.6084/m9.figshare.1466767.v28
title: Procedure for keeping into account solar reflections from obstructions in ESP-r on the basis of irradiance ratios. (2015-16)

description: Program which brings into account the reflective effect of solar obstructions on solar gains in the ESP-r building models on the basis of irradiance ratios. Those ratios are obtained combining the direct radiation on a surface and the total radiation on it calculated by the means of the Radiance raytracer. Modish can be installed from the command line by typing: sudo cpanm Sim::OPT::Modish
DOI: 10.17632/j7mxzzbhc5.1
title: Dupilumab shows long-term effectiveness in a large cohort of treatment-refractory atopic dermatitis patients in daily practice: 52-weeks results from the Dutch BioDay registry

description: Dupilumab shows long-term effectiveness in a large cohort of treatment-refractory atopic dermatitis patients in daily practice: 52-weeks results from the Dutch BioDay registry
DOI: 10.6084/m9.figshare.1464990
title: L35Ae interaction with HEK293 cells

description: HEK293 cells were incubated with 6xHis-tagged L35Ae or anti-hCCR7 Fab17 used as a negative control, followed by immunostaining and flow cytometry analysis. FACS buffer contained PBS, 150 mM NaCl, pH 7.0, 1-1.5% calf serum, and 0.01% NaN3. R-Phycoerythrin fluorescence was excited at 532 nm and detected at 580-583 nm.
DOI: 10.6084/m9.figshare.1464988
title: Gel-filtration chromatography data for L35Ae samples

description: Gel-filtration chromatography data for L35Ae samples (3.5-3.7 mg/ml) and molecular mass standards at room temperature (Superdex 75 10/300 GL column). The elution profiles of L35Ae samples are shown in the inset. The open circles corresponding to elution times of the mass standards were described by linear function (solid line). The apparent molecular masses of L35Ae samples were determined by comparison of their elution times with the calibration line, as indicated by dashed arrowed lines.
DOI: 10.6084/m9.figshare.1464992.v1
title: Stability of L35Ae samples towards thermal and chemical denaturation

description: GuHCl-induced changes in position of tryptophan fluorescence spectrum maximum of L35Ae. Theoretical fits to the experimental data using Boltzmann function were made. Buffer contained PBS, 150 mM NaCl, pH 7.0. Protein concentration was 3 µM.
DOI: 10.6084/m9.figshare.1465014.v4
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.