cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5241352
title: Erratum: Gonadotropin-Inhibitory Hormone Is a Hypothalamic Peptide That Provides a Molecular Switch between Reproduction and Feeding

description: <b><i>Objective:</i></b> Gonadotropin-inhibitory hormone (GnIH)-3 is a neuropeptide that plays a major role in the regulation of reproduction and feeding in mammals. <b><i>Materials and Methods:</i></b> We measured endocrine and behavioural parameters of reproduction in sheep, and sexual behaviour in sheep, mice and cynomolgus monkeys. In addition, GnIH gene expression (in situ hybridization) was examined in ewes, and effects of GnIH-3 on food intake and energy expenditure were measured in various species. GnIH-3 was infused (i.v.) into ewes after an i.m. injection of estradiol benzoate to determine whether the peptide blocks the surge in luteinizing hormone (LH) secretion. <b><i>Results:</i></b> GnIH gene expression was reduced in the preovulatory period in ewes. Infusion (i.v.) of GnIH-3 blocked the estrogen-induced LH surge (in ewes). Intracerebroventricular infusion had no effect on female or male sexual behaviour in each of the three species, but increased food intake. There were no effects on energy expenditure in sheep or rats. GnIH increased fos protein (immunohistochemistry) was seen in orexigenic neurons (in sheep and rats), but also in anorexigenic neurons (in sheep). <b><i>Conclusions:</i></b> GnIH-3 reduces reproductive hormone levels and increases food intake in mammals without reducing energy expenditure. There is minimal effect on reproductive behaviour. The dual effect on reproduction and feeding suggests that GnIH-3 provides a molecular switch between these two functions. Blockade of the positive feedback effect of estrogen with parenteral infusion indicates that this peptide may have utility as a blocker of reproductive function in mammals.
DOI: 10.6084/m9.figshare.5241355
title: Erratum: Zertifizierung und Zentrenbildung aus der Sicht der Deutschen Gesellschaft für Allgemein- und Viszeralchirurgie (DGAV)

description: Die Deutsche Gesellschaft für Allgemein- und Viszeralchirurgie (DGAV) hat zur Verbesserung der Qualität in den chirurgischen Abteilungen ein Zertifizierungssystem entwickelt. Kennzeichen dieses Systems ist die Darstellung umschriebener chirurgischer Bereiche, die mit einer überschaubaren Anzahl von Items (&lt;50) charakterisiert werden können. Es sind Zertifizierungsstufen mit unterschiedlichen Anforderungen geschaffen worden: Kompetenz-, Referenz- und Exzellenzzentren. Am Kompetenzzentrum kann der Patient sicher sein, dass er eine leitliniengerechte Behandlung in überprüften Strukturen und Prozessen erhält. Referenzzentren verfügen darüber hinaus über Kompetenzen in Weiter- und Fortbildung sowie in wissenschaftlichen Aktivitäten durch Teilnahme an Studien. Exzellenzzentren stellen in jeder Hinsicht die führenden Institutionen in Deutschland dar. Ein Zertifikat hat eine Gültigkeit von 3 Jahren und muss dann durch eine Rezertifizierung mit Audit erneuert werden. Bei der Erstzertifizierung werden die Strukturen und Prozesse der Abteilungen überprüft. Eine Rezertifizierung ist nur möglich, wenn Daten zur Ergebnisqualität vorgelegt werden können. Der Zertifizierungsprozess wird auf der Grundlage der DIN ISO 17021 von der SAVC GmbH, Berlin, vorgenommen. Nach dem System der DGAV sind von sieben Arbeitsgemeinschaften Zertifizierungskriterien entwickelt worden. Inzwischen konnten Zertifikate an insgesamt 230 chirurgische Abteilungen vergeben werden. 10,9% der Anträge mussten abgelehnt werden.
DOI: 10.6084/m9.figshare.5241358
title: Erratum: The Effect of Inactivated <i>Lactobacillus</i> LB Fermented Culture Medium on Symptom Severity: Observational Investigation in 297 Patients with Diarrhea-Predominant Irritable Bowel Syndrome

description: <i>Introduction:</i> Little is known about the intensity of symptoms of diarrhea-predominant IBS (IBS-D) or the consequences of the disease on patients’ health-related quality of life (HRQOL). This observational investigation assessed the symptoms (abdominal pain, bloating, number of stools per day, and stool consistency), impact on HRQOL, and consequence on anal continence in 297 patients with IBS-D before and after 1 month of probiotic treatment with Lacteol (inactivated <i>Lactobacillus </i>LB plus fermented culture medium). <i>Methods:</i> Functional assessment using a standardized visual analogue scale in order to quantify abdominal pain, bloating, and quality of life before and after 1 month of treatment with 2 capsules/day of Lacteol. The number of symptomatic days per week, number of stools, consistency of stools, secondary fecal incontinence rate, and potential trigger effect of food were quantified. A χ<sup>2</sup> test was used to compare qualitative data and the variance of quantitative criteria was analyzed. <i>Results:</i> The pain score decreased from 4.46 ± 0.15 on a scale of 0–10 before treatment to 2.8 ± 0.14 after treatment (p &lt; 0.0001). Bloating decreased from 4.49 ± 0.18 to 2.5 ± 0.15 on a scale of 0–10 (p &lt; 0.0001). The HRQOL score, which is inversely correlated with quality of life, decreased from 5.99 ± 0.14 to 3.92 ± 0.16 (p &lt; 0.0001). In this cohort study, the fecal incontinence rate secondary to diarrhea was clearly higher than that of the general population: 18% versus a prevalence of 9–10%, according to different studies. The mean number of stools per week decreased from 17.59 to 12.83 after treatment (p &lt; 0.0001). Before treatment, 54% of patients had watery stools and 46% had smooth stools; at the end of treatment, only 18.5% of patients still had watery stools, and 34% had normal stools. 52% of patients attributed their symptoms to their diet: 34% to vegetables, 29% to fruit, 15% to milk, 15% to fat, 6% to peppers and spices, and 4% to sugar. <i>Conclusion:</i> This observational investigation shed new light on patients with IBS-D, the HRQOL of which is altered by a fecal incontinence rate twice as high as that of the general population. Correlation with diet is confirmed by 1 out of 2 patients reporting poor tolerance of fiber and dairy products. Nutritional management should thus be part of these patients’ treatment. Inactivated <i>Lactobacillus</i> LB plus fermented culture medium is a probiotic drug that has been used by physicians for a long time to treat patients with diarrhea. Strongly concentrated, it has no side effects and seems to help these patients. Due to a strong placebo effect in patients with this pathology, however, a controlled study is necessary to confirm this result.
DOI: 10.6084/m9.figshare.5241349
title: Erratum: Can Mortality Data Provide Reliable Indicators for Creutzfeldt-Jakob Disease Surveillance? A Study in France from 2000 to 2008

description: <i>Background:</i> Surveillance of Creutzfeldt-Jakob disease (CJD) is still an important issue because of the variant CJD epidemic, which is in decline and also because of the emergence of novel forms of animal transmissible spongiform encephalopathy with zoonotic potential and the risk of nosocomial and blood transfusion-related transmission. Active surveillance has been implemented in most European countries and requires important human resources and funding. Here, we studied whether national mortality and morbidity statistics can be used as reliable indicators. <i>Methods:</i> CJD data collected by the French national CJD surveillance centre were compared with data registered in the national mortality statistics. <i>Results:</i> From 2000 to 2008, the two sources reported fairly similar numbers of CJD deaths. However, analysis of individual data showed important between-sources disagreement. Nearly 24% of CJD reported by the mortality register were false-positive diagnoses and 21.6% of the CJD cases diagnosed by the surveillance centre were not registered as CJD in the national mortality statistics. One out of 22 variant CJD cases was not reported as having any type of CJD in the mortality statistics. <i>Conclusions:</i>These findings raise doubt about the possibility of a reliable CJD surveillance only based on mortality data.
DOI: 10.6084/m9.figshare.5241346
title: Erratum: Renal Function and Solitary Kidney Disease: Wilms Tumour Survivors versus Patients with Unilateral Renal Agenesis

description: <i>Aims:</i> To test the hypothesis that Wilms tumour survivors (WTs) experience increased disturbance in renal function, even after prompt treatment, compared to patients with unilateral renal agenesis (URA). <i>Methods:</i> To assess the renal function of 30 WTs and 17 individuals with URA, the estimated glomerular filtration rate (eGFR) was calculated using the Schwartz and Filler formulas as well as the new Schwartz equation for chronic kidney disease. To measure kidney damage, serum levels and urine excretion of β<sub>2</sub>-microglobulin (B2M), cystatin C (Cys C), neutrophil gelatinase-associated lipocalin (NGAL) were tested, N-acetyl-β-glucosaminidase (NAG), and albumin urine excretion and urine sediment were examined. Blood pressure was measured. <i>Results:</i> No differences were found between the groups in terms of eGFR, serum Cys C, B2M and NGAL concentrations. The urine excretion of Cys C, NGAL and NAG was similar in both groups. URA patients had higher B2M excretion than WTs. Arterial hypertension was present in 7/30 (23%) WTs and 1/17 (6%) patients with URA. <i>Conclusions:</i> WTs have similar eGFR to individuals with URA and are more likely to have arterial hypertension. The patients with URA have signs of tubular damage. This study demonstrates the need for nephrological monitoring of individuals with a single kidney.
DOI: 10.6084/m9.figshare.5241352.v1
title: Erratum: Gonadotropin-Inhibitory Hormone Is a Hypothalamic Peptide That Provides a Molecular Switch between Reproduction and Feeding

description: <b><i>Objective:</i></b> Gonadotropin-inhibitory hormone (GnIH)-3 is a neuropeptide that plays a major role in the regulation of reproduction and feeding in mammals. <b><i>Materials and Methods:</i></b> We measured endocrine and behavioural parameters of reproduction in sheep, and sexual behaviour in sheep, mice and cynomolgus monkeys. In addition, GnIH gene expression (in situ hybridization) was examined in ewes, and effects of GnIH-3 on food intake and energy expenditure were measured in various species. GnIH-3 was infused (i.v.) into ewes after an i.m. injection of estradiol benzoate to determine whether the peptide blocks the surge in luteinizing hormone (LH) secretion. <b><i>Results:</i></b> GnIH gene expression was reduced in the preovulatory period in ewes. Infusion (i.v.) of GnIH-3 blocked the estrogen-induced LH surge (in ewes). Intracerebroventricular infusion had no effect on female or male sexual behaviour in each of the three species, but increased food intake. There were no effects on energy expenditure in sheep or rats. GnIH increased fos protein (immunohistochemistry) was seen in orexigenic neurons (in sheep and rats), but also in anorexigenic neurons (in sheep). <b><i>Conclusions:</i></b> GnIH-3 reduces reproductive hormone levels and increases food intake in mammals without reducing energy expenditure. There is minimal effect on reproductive behaviour. The dual effect on reproduction and feeding suggests that GnIH-3 provides a molecular switch between these two functions. Blockade of the positive feedback effect of estrogen with parenteral infusion indicates that this peptide may have utility as a blocker of reproductive function in mammals.
DOI: 10.6084/m9.figshare.5241355.v1
title: Erratum: Zertifizierung und Zentrenbildung aus der Sicht der Deutschen Gesellschaft für Allgemein- und Viszeralchirurgie (DGAV)

description: Die Deutsche Gesellschaft für Allgemein- und Viszeralchirurgie (DGAV) hat zur Verbesserung der Qualität in den chirurgischen Abteilungen ein Zertifizierungssystem entwickelt. Kennzeichen dieses Systems ist die Darstellung umschriebener chirurgischer Bereiche, die mit einer überschaubaren Anzahl von Items (&lt;50) charakterisiert werden können. Es sind Zertifizierungsstufen mit unterschiedlichen Anforderungen geschaffen worden: Kompetenz-, Referenz- und Exzellenzzentren. Am Kompetenzzentrum kann der Patient sicher sein, dass er eine leitliniengerechte Behandlung in überprüften Strukturen und Prozessen erhält. Referenzzentren verfügen darüber hinaus über Kompetenzen in Weiter- und Fortbildung sowie in wissenschaftlichen Aktivitäten durch Teilnahme an Studien. Exzellenzzentren stellen in jeder Hinsicht die führenden Institutionen in Deutschland dar. Ein Zertifikat hat eine Gültigkeit von 3 Jahren und muss dann durch eine Rezertifizierung mit Audit erneuert werden. Bei der Erstzertifizierung werden die Strukturen und Prozesse der Abteilungen überprüft. Eine Rezertifizierung ist nur möglich, wenn Daten zur Ergebnisqualität vorgelegt werden können. Der Zertifizierungsprozess wird auf der Grundlage der DIN ISO 17021 von der SAVC GmbH, Berlin, vorgenommen. Nach dem System der DGAV sind von sieben Arbeitsgemeinschaften Zertifizierungskriterien entwickelt worden. Inzwischen konnten Zertifikate an insgesamt 230 chirurgische Abteilungen vergeben werden. 10,9% der Anträge mussten abgelehnt werden.
DOI: 10.6084/m9.figshare.5241343
title: Erratum: A Double-Blind, Placebo-Controlled Study of Aripiprazole Adjunctive to Antidepressant Therapy among Depressed Outpatients with Inadequate Response to Prior Antidepressant Therapy (ADAPT-A Study)

description: <i>Background:</i> We assessed the efficacy of low-dose aripiprazole added to antidepressant therapy (ADT) in major depressive disorder (MDD) patients with inadequate response to prior ADT. <i>Methods:</i> As per the sequential parallel comparison design, 225 MDD subjects were randomized to adjunctive treatment with aripiprazole 2 mg/day or placebo across two 30-day phases, with a 2:3:3 randomization ratio to drug/drug (aripiprazole 2 mg/day in phase 1; 5 mg/day in phase 2), placebo/placebo (placebo in both phases), and placebo/drug (placebo in phase 1; aripiprazole 2 mg/day in phase 2). Eligible subjects were patients whose MDD was independently deemed ‘valid’ with SAFER criteria. Subjects had been receiving ADT for ≥8 weeks, and had inadequate response to ≥1 and &lt;4 adequate ADTs in the current episode, as defined by the Antidepressant Treatment Response Questionnaire. <i>Results:</i> The pooled, weighted response difference between aripiprazole 2 mg/day and placebo in the two phases was 5.6% (p = 0.18; NS). The aripiprazole 2 mg/day-placebo difference on the Montgomery-Asberg Depression Rating Scale pooled across the two phases was –1.51 (p = 0.065; NS). Other secondary endpoint analyses showed nonsignificant pooled differences favoring aripiprazole over placebo. Of the 225 randomized subjects in phase 1, 2 dropped out in both arms, while in phase 2, of 138 phase 1 placebo nonresponders, 9 dropped out on aripiprazole and 5 on placebo. There were only minimal differences in adverse event rates between treatments, except for constipation, weight gain, and dry mouth, more common on aripiprazole. <i>Conclusions:</i> This study provides clear support for the tolerability of low-dose aripiprazole as an ADT-augmenting agent, with marginal efficacy.
DOI: 10.6084/m9.figshare.5241346.v1
title: Erratum: Renal Function and Solitary Kidney Disease: Wilms Tumour Survivors versus Patients with Unilateral Renal Agenesis

description: <i>Aims:</i> To test the hypothesis that Wilms tumour survivors (WTs) experience increased disturbance in renal function, even after prompt treatment, compared to patients with unilateral renal agenesis (URA). <i>Methods:</i> To assess the renal function of 30 WTs and 17 individuals with URA, the estimated glomerular filtration rate (eGFR) was calculated using the Schwartz and Filler formulas as well as the new Schwartz equation for chronic kidney disease. To measure kidney damage, serum levels and urine excretion of β<sub>2</sub>-microglobulin (B2M), cystatin C (Cys C), neutrophil gelatinase-associated lipocalin (NGAL) were tested, N-acetyl-β-glucosaminidase (NAG), and albumin urine excretion and urine sediment were examined. Blood pressure was measured. <i>Results:</i> No differences were found between the groups in terms of eGFR, serum Cys C, B2M and NGAL concentrations. The urine excretion of Cys C, NGAL and NAG was similar in both groups. URA patients had higher B2M excretion than WTs. Arterial hypertension was present in 7/30 (23%) WTs and 1/17 (6%) patients with URA. <i>Conclusions:</i> WTs have similar eGFR to individuals with URA and are more likely to have arterial hypertension. The patients with URA have signs of tubular damage. This study demonstrates the need for nephrological monitoring of individuals with a single kidney.
DOI: 10.6084/m9.figshare.5241349.v1
title: Erratum: Can Mortality Data Provide Reliable Indicators for Creutzfeldt-Jakob Disease Surveillance? A Study in France from 2000 to 2008

description: <i>Background:</i> Surveillance of Creutzfeldt-Jakob disease (CJD) is still an important issue because of the variant CJD epidemic, which is in decline and also because of the emergence of novel forms of animal transmissible spongiform encephalopathy with zoonotic potential and the risk of nosocomial and blood transfusion-related transmission. Active surveillance has been implemented in most European countries and requires important human resources and funding. Here, we studied whether national mortality and morbidity statistics can be used as reliable indicators. <i>Methods:</i> CJD data collected by the French national CJD surveillance centre were compared with data registered in the national mortality statistics. <i>Results:</i> From 2000 to 2008, the two sources reported fairly similar numbers of CJD deaths. However, analysis of individual data showed important between-sources disagreement. Nearly 24% of CJD reported by the mortality register were false-positive diagnoses and 21.6% of the CJD cases diagnosed by the surveillance centre were not registered as CJD in the national mortality statistics. One out of 22 variant CJD cases was not reported as having any type of CJD in the mortality statistics. <i>Conclusions:</i>These findings raise doubt about the possibility of a reliable CJD surveillance only based on mortality data.
DOI: 10.6084/m9.figshare.5241331
title: Erratum: Skin Cancer Concerns and Genetic Risk Information-Seeking in Primary Care

description: <i>Background:</i> Genomic testing for common genetic variants associated with skin cancer risk could enable personalized risk feedback to motivate skin cancer screening and sun protection. <i>Methods:</i> In a cross-sectional study, we investigated whether skin cancer cognitions and behavioral factors, sociodemographics, family factors, and health information-seeking were related to perceived importance of learning about how (a) genes and (b) health habits affect personal health risks using classification and regression trees (CART). <i>Results:</i> The sample (n = 1,772) was collected in a large health maintenance organization as part of the Multiplex Initiative, ranged in age from 25–40, was 53% female, 41% Caucasian, and 59% African-American. Most reported that they placed somewhat to very high importance on learning about how genes (79%) and health habits (88%) affect their health risks. Social influence actors were associated with information-seeking about genes and health habits. Awareness of family history was associated with importance of health habit, but not genetic, information-seeking. <i>Conclusions:</i> The investment of family and friends in health promotion may be a primary motivator for prioritizing information-seeking about how genes and health habits affect personal health risks and may contribute to the personal value, or personal utility, of risk information. Individuals who seek such risk information may be receptive to interventions aimed to maximize the social implications of healthy lifestyle change to reduce their health risks.
DOI: 10.6084/m9.figshare.5241334
title: Erratum: Genome-Wide Association Studies for Bivariate Sparse Longitudinal Data

description: <i>Objective:</i> Longitudinal measurements with bivariate response have been analyzed by several authors using two separate models for each response. However, for most of the biological or medical experiments, the two responses are highly correlated and hence a separate model for each response might not be a desirable way to analyze such data. A single model considering a bivariate response provides a more powerful inference as the correlation between the responses is modeled appropriately. In this article, we propose a dynamic statistical model to detect the genes controlling human blood pressure (systolic and diastolic). <i>Methods:</i> By modeling the mean function with orthogonal Legendre polynomials and the covariance matrix with a stationary parametric structure, we incorporate the statistical ideas in <i>functional genome-wide association studies </i>to detect SNPs which have significant control on human blood pressure. The traditional false discovery rate is used for multiple comparisons. <i>Results:</i> We analyze the data from the Framingham Heart Study to detect such SNPs by appropriately considering gender-gene interaction. We detect 8 SNPs for males and 7 for females which are most significant in controlling blood pressure. The genotype-specific mean curves and additive and dominant effects over time are shown for each significant SNP for both genders. Simulation studies are performed to examine the statistical properties of our model. The current model will be extremely useful in detecting genes controlling different traits and diseases for humans or non-human subjects.
DOI: 10.6084/m9.figshare.5241337
title: Erratum: Paclitaxel, Ifosfamide and Cisplatin (TIP) Beyond Its Original Indication for Salvage Treatment of Germ Cell Tumors

description: Medicine
DOI: 10.6084/m9.figshare.5241340
title: Erratum: Comparative Evaluation of Different Cell Lysis and Extraction Methods for Studying Benzo(a)pyrene Metabolism in HT-29 Colon Cancer Cell Cultures

description: Lysis and extraction of cells are essential sample processing steps for investigations pertaining to metabolism of xenobiotics in cell culture studies. Of particular importance to these procedures are maintaining high lysis efficiency and analyte integrity as they influence the qualitative and quantitative distribution of drug and toxicant metabolites in the intra- and extracellular milieus. In this study we have compared the efficiency of different procedures viz. homogenization, sonication, bead beating, and molecular grinding resin treatment for disruption of HT-29 colon cells exposed to benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) compound and a suspected colon carcinogen. Also, we have evaluated the efficiency of various procedures for extracting BaP parent compound/metabolites from colon cells and culture media prior to High Performance Liquid Chromatography (HPLC) analyses. The extraction procedures include solid phase extraction, solid-supported liquid- liquid extraction, liquid-liquid extraction, and homogeneous liquid- liquid extraction. Our findings showed that bead-beating in combination with detergent treatment of cell pellet coupled with liquid-liquid extraction yielded greater concentrations of BaP metabolites compared to the other methods employed. Our method optimization strategy revealed that disruption of HT-29 colon cells by a combination of mechanical and chemical lysis followed by liquid-liquid extraction is efficient and robust enough for analyzing BaP metabolites from cell culture studies.