DOI: 10.5281/zenodo.14939397
title: Particulate inorganic carbon concentration in the ocean: Dataset for protocol recommendations

description: This dataset contains Particulate Inorganic Carbon (PIC) concentrations from coccolithophore cultures, and associated metadata, for evaluating sample processing protocols. These data were collected on polycarbonate filters, with two different filter pore sizes, and two different filter rinse types. To achieve a range of  PIC concentrations, we sampled five different culture strains with diverse coccolith morphologies. For more details on dataset preparation and processing, see the Original Publication Citation and Data Processing Workflow below.

 
description: Data Processing Workflow

Details and supporting code for the workflow to produce the dataset files are available on GitHub: https://github.com/MarineOpticsLab/PIC-DSP-paper.
description: Dataset Details

 

There is one CSV file in this dataset: PIC-dataset-for-protocol-recommendations.csv. The table below (Table 1) describes each column in the CSV file.

 

 

 

 

Table 1: Column descriptions for the data in PIC-dataset-for-protocol-recommendations.csv

 






Column Header



Units



Description





Code



-



Code summarising each culture, filter pore size and rinse combination. The first letter indicates the culture, the second the filter pore size, and the third is the rinse type. The number is replicate number. See Table 2 for further breakdown of these codes.





Tube Number



-



Number assigned to each sample tube





Culture



-



Strain number





Name



- 



Species





Ploidity



-



Number of complete sets of chromosomes (n = 1 set, 2n = 2 sets)





CaCo3



- 



If the sample is calcified or not (i.e. contains PIC)





Filter



mm



Filter pore size





Rinse



-



Rinse agent





Comment on filtration



-



Notes from ICP-MS facility technician





Ca (ug/L)



mg/L



Concentration of calcium detected by ICP-MS 





Mg (ug/L)



mg/L



Concentration of calcium detected by ICP-MS





Sr (ug/L)



mg/L



Concentration of strontium detected by ICP-MS





Na (ug/L)



mg/L



Concentration of sodium detected ICP-MS





Ca sw corr



mg/L



Salt-water corrected concentration of calcium





Ca sw + V corr



mg/L



Salt-water and volume corrected concentration of calcium





PIC mmol/m3



mmol/m3



Blank corrected PIC concentration





PIC ug/l



mg/L



Blank corrected PIC concentration





PIC blank mean mmol/m3



mmol/m3



PIC blank concentration (mean of 3 replicates)





PIC blank mean ug/l



mg/L



PIC blank concentration (mean of 3 replicates)





PIC blank stdev mmol/m3



mmol/m3



Standard deviation of the PIC blank concentrations





PIC blank stdev ug/l



mg/L



Standard deviation of the PIC blank concentrations





PIC blank %



%



PIC blank relative to blank corrected PIC concentration





 

Table 2: Sample code descriptors






Symbol



Description





L



Calcidiscus leptoporus, diploid, heterococcolith (RCC1164)





C



Calcidiscus leptoporus, haploid, holococcolith (RCC1151)





N



Calcidiscus leptoporus, haploid, non-calcifying (RCC1129)





U



Umbilicosphaera foliosa (RCC1472)





S



Syracosphaera pulchra (MIR02)





A



Filter pore size = 0.8 mm





B



Filter pore size = 0.4 mm





X



Rinse = NH4OH





Y



Rinse = K2B4O7