DOI: 10.5061/dryad.jdfn2z3nz
title: Data from: The effect of inactivated bacteria on the redox status of larvae of the wax moth Galleria mellonella

description: Insects’ defenses can be directed at both live and inactivated pathogens.
 Recognition of pathogen-associated structures leads to modulation of
 innate immune responses and oxidative stress in the host. The article
 presents results of experiments showing that inactivated vegetative cells
 of Bacillus thuringiensis, B. subtilis, and B. cereus can modulate immune
 responses in Galleria mellonella in the absence of damage to tissues of
 the midgut. In the first hours after feeding on Bacillus spp., an increase
 in the number of immunocompetent hemocytes was observed. Phenoloxidase
 activity in the hemolymph of wax moth larvae was inhibited, which is
 confirmed by the suppression of the production of reactive oxygen species
 in hemolymph. In addition, it was found that the antioxidant response to
 the bacterial inoculation in the midgut and hemolymph is different.
 Probably, the local antioxidant defense system is probably configured in
 such a way that the level of reactive oxygen species increases rapidly to
 perform useful biological functions and decreases just as quickly,
 minimizing oxidative damage.
description: # Data from: The effect of inactivated bacteria on the redox status of
 larvae of the wax moth Galleria mellonella Dataset DOI:
 [10.5061/dryad.jdfn2z3nz](10.5061/dryad.jdfn2z3nz) ## Description of the
 data and file structure Data set for the manuscript by Jana L. Vorontsova
 et al. “Effect of inactivated bacteria on the redox status of *Galleria
 mellonella* wax moth larvae”, including (i) survival of *G. mellonella*
 wax moth larvae after *per os* administration of inactivated vegetative
 cells of *B. thuringiensis*, *B. subtilis* or *B. cereus*, (ii) total
 hemocyte count (THC) (a) and phenoloxidase (PO) activity (b) in the
 hemolymph of *G. mellonella* wax moth larvae 12 and 24 h after *per os*
 administration of inactivated vegetative cells of *B. thuringiensis*, *B.
 subtilis* or *B. cereus*, (iii) the magnitude of reactive oxygen species
 (ROS) production (a, c) and total antioxidant activity (TAC) (b, d) in the
 hemolymph (a, b) and midgut (c, d) of *G. mellonella* wax moth larvae 12
 and 24 h after *per os* administration of *B. thuringiensis*, *B.
 subtilis* or *B. cereus* IBVCs, and (iv) survival of *G. mellonella* wax
 moth larvae after *per os* administration of (a) active vegetative cells
 and (b) 6-day-old bacterial cultures of *B. thuringiensis*, *B. subtilis*
 or *B. cereus*. ### Files and variables *
 Yana_L._Vorontsova_Figure_1_The_survival_of_wax_moth.xlsx - Yana L.
 Vorontsova Figure 1 The survival of wax moth - The dataset for Figure 1
 The survival of wax moth *G. mellonella* larvae after *per os*
 administration of inactivated vegetative cells of *B. thuringiensis*, *B.
 subtilis*, or *B. cereus*. Missing values (empty cells) will be marked
 with "n/a". -
 Yana_L._Vorontsova_Figure_2a_The_total_hemocyte_count.xlsx - Yana L.
 Vorontsova Figure 2a The total hemocyte count - The dataset for Figure
 2a The total hemocyte count (THC) (a) and activity of phenoloxidases (PO)
 (b) in the hemolymph of wax moth *G. mellonella* larvae at 12 and 24 h
 after *per os* administration of IBVCs of *B. thuringiensis*, *B.
 subtilis*, or *B. cereus. * *
 Yana_L._Vorontsova_Figure_2b_Activity_of_phenoloxidase.xlsx - Yana L.
 Vorontsova Figure 2b Activity of phenoloxidase - The dataset for Figure 2b
 The total hemocyte count (THC) (a) and activity of phenoloxidases (PO) (b)
 in the hemolymph of wax moth *G. mellonella* larvae at 12 and 24 h after
 *per os* administration of IBVCs of *B. thuringiensis*, *B. subtilis*, or
 *B. cereus. *Missing values (empty cells) will be marked with
 "n/a". - Yana_L._Vorontsova_Figure_3a_Generation_of_ROS.xlsx -
 Yana L. Vorontsova Figure 3a Generation of ROS - The dataset for Figure
 3a The magnitude of production of reactive oxygen species (ROS) (a, c) and
 total antioxidant activity (TAC) (b, d) in hemolymph (a, b) and in the
 midgut (c, d) of wax moth *G. mellonella* larvae at 12 and 24 h after *per
 os* administration of IBVCs of *B. thuringiensis*, *B. subtilis*, or *B.
 cereus.*  *
 Yana_L._Vorontsova_Figure_3b_Total_antioxidant_activity_in_hemolymph.xlsx
 - Yana L. Vorontsova Figure 3b Total antioxidant activity in hemolymph
 - The dataset for Figure 3b The magnitude of production of reactive oxygen
 species (ROS) (a, c) and total antioxidant activity (TAC) (b, d) in
 hemolymph (a, b) and in the midgut (c, d) of wax moth *G. mellonella*
 larvae at 12 and 24 h after *per os* administration of IBVCs of *B.
 thuringiensis*, *B. subtilis*, or *B. cereus.*  -
 Yana_L._Vorontsova_Figure_3c_Generation_of_ROS_in_midgut.xlsx - Yana L.
 Vorontsova Figure 3c Generation of ROS in midgut - The dataset for Figure
 3b The magnitude of production of reactive oxygen species (ROS) (a, c) and
 total antioxidant activity (TAC) (b, d) in hemolymph (a, b) and in the
 midgut (c, d) of wax moth *G. mellonella* larvae at 12 and 24 h after *per
 os* administration of IBVCs of *B. thuringiensis*, *B. subtilis*, or *B.
 cereus.*  *
 Yana_L._Vorontsova_Figure_3d_Total_antioxidant_activity_im_midgut.xlsx -
 Yana L. Vorontsova Figure 3d Total antioxidant activity im midgut - The
 dataset for Figure 3b The magnitude of production of reactive oxygen
 species (ROS) (a, c) and total antioxidant activity (TAC) (b, d) in
 hemolymph (a, b) and in the midgut (c, d) of wax moth *G. mellonella*
 larvae at 12 and 24 h after *per os* administration of IBVCs of *B.
 thuringiensis*, *B. subtilis*, or *B. cereus.* Missing values (empty
 cells) will be marked with "n/a". -
 Yana_L._Vorontsova_Figure_S1a.xlsx - Yana L. Vorontsova Figure S1a - The
 dataset for Figure S1a The survival of *G. mellonella* wax moth larvae
 after *per os* administration of (a) active vegetative cells and (b)
 6-day-old bacterial cultures of *B. thuringiensis*, *B. subtilis* or *B.
 cereus*. * Yana_L._Vorontsova_Figure_S1b.xlsx - Yana L. Vorontsova Figure
 S1b - The dataset for Figure S1b The survival of *G. mellonella* wax moth
 larvae after *per os* administration of (a) active vegetative cells and
 (b) 6-day-old bacterial cultures of *B. thuringiensis*, *B. subtilis* or
 *B. cereus*. ## Code/software Microsoft Office (Excel)