DOI: 10.5061/dryad.jdfn2z3qh
title: Bacterial-driven odorant receptor expression shifts alter olfactory behavior in the oriental fruit fly

description: This work reveals a previously unknown mechanism by which gut microbiota
 modulate insect sensory ecology, demonstrating that Enterobacter sp. MU-1,
 a gut symbiont of the invasive pest Bactrocera dorsalis, reprograms host
 olfactory behavior through specific suppression of odorant receptor OR67c.
 Our integrated approach combining behavioral assays, transcriptomics, and
 functional validation establishes a novel paradigm of tripartite interplay
 between gut microbiota, chemoreceptor tuning, and behavioral adaptation.
 Key findings include: MU-1 colonization converts avoidance behavior to
 attraction in axenic flies (P &lt; 0.01, Figure 1), while
 RNAi-mediated knockdown of OR67c reverses this behavioral shift (55% gene
 downregulation, P &lt; 0.05, Figure 4). Temporal qPCR analysis
 confirms that MU-1 specifically suppresses OR67c expression (FPKM &gt;
 2-fold change in antibiotic-treated vs. recolonized flies, Figure 3), with
 no equivalent effects on other olfactory receptors. This
 microbiota-chemosensory axis represents a significant advance in
 understanding how symbiotic bacteria fine-tune insect behavioral
 plasticity—a phenomenon with critical implications for developing novel
 management strategies against this globally invasive species.
description: # Bacterial-driven odorant receptor expression shifts alter olfactory
 behavior in the oriental fruit fly Dataset DOI:
 [10.5061/dryad.jdfn2z3qh](https://doi.org/10.5061/dryad.jdfn2z3qh) ##
 Description of the data and file structure We have submitted all the raw
 data used for plotting. All the content mentioned is included in one Excel
 file. Each sheet in the file corresponds to the data used for each figure
 in the original article. ### Files and variables #### File:
 Data_for_publication.xlsx **Description:** Data for Figure 1A:Y-tube
 olfactory behavior of *B. dorsalis*. The numbers in the table represent
 the number of insects that made the corresponding choices. Data for Figure
 1B:Behavioral analysis in a T-shaped tube.The numbers in the table
 indicate the quantity of insects that made the corresponding choices, and
 the calculation of the selection index is also included in the table. Data
 for Figure 2:The data on larval body length and weight. The numbers in the
 second row represent the initial concentrations of the bacterial
 suspension, in units of CFU/mL. Data for Figure 3A:Data on the
 corresponding odorant receptors screened from transcriptome sequencing
 data. The values used for plotting are the FPKM values of the genes. Data
 for Figure 3B:The numbers in the table are the raw data used for plotting.
 Data for Figure 4:Behavioral analysis in a T-shaped tube.The numbers in
 the table indicate the quantity of insects that made the corresponding
 choices, and the calculation of the selection index is also included in
 the table. Data for Figure S1:The phylogenetic tree was generated using
 MEGA software, with the nucleotide sequences used provided in the table,
 and the other sequences available in the NCBI database. Data for Figure
 S2:The data in the table represent the bacterial concentration, expressed
 in colony-forming units per larva. Data for Figure S3:The data in the
 table represent the proportion of *B. dorsalis*. that selected the
 corresponding odor source. Data for Figure S4:The data in the table
 represent the bacterial concentration, with the unit being CFU/mL. Data
 for Figure S5:The data in the table comprise the complete set of
 differentially expressed genes required for generating the volcano
 plot.The plot was generated via the cloud platform :
 [https://magic-plus.novogene.com/#/](https://magic-plus.novogene.com/#/).
 Data for Figure S6:The data in the table represent the relative expression
 level of OR67c. ##### Variables * Figure 1A: **Behavioral analysis in a Y
 tube**:Experiment name. **source 1**:The number of insects entering this
 arm of the Y-tube (individuals). The second row describes the odor used in
 this arm. **source 2**:The number of insects entering this arm of the
 Y-tube (individuals). The second row describes the odor used in this arm.
 * Figure 1B: **Behavioral analysis in a T-shaped tube**:Experiment name. 
 * Figure 2: **larval body length (cm)**：Data from measurements of larval
 body length (cm). **larval weight (mg)**: Data from measurements of larval
 body weight (mg). * Figure 3A: **gene_id**:The gene's identification
 number on NCBI. **AT1_count to CR3_count**:The count of reads that are
 aligned to a gene provides a direct, unnormalized estimate of the
 gene's expression level.The alphanumeric codes (e.g., AT1,CR1)
 represent different experimental groups. **AT1_fpkm to
 CR3_fpkm**:Fragments Per Kilobase of transcript per Million mapped
 fragments.The alphanumeric codes (e.g., AT1,CR1) represent different
 experimental groups. * Figure 3B: **OR83a,OR67d-like,OR67c**: gene
 name. **CR,AT,CR+3d,RC**:CR:conventional-reared ;AT:antibiotic-treated
 ;CR+3d:continuously feed CR group for an additional 3 days ;RC:MU-1
 recolonization. * Figure 4:**Behavioral analysis in a T-shaped
 tube**:Experiment name.  * Figure S1: **sequence: **Base sequence. *
 Figure S2: **Control,Anti**: Group name. * Figure S3: **Control,Anti**:
 Group name. * Figure S4: **Control,Anti,MU-1 recolonized**:Group name. *
 Figure S5: **gene_id**:The gene's identification number on NCBI.
 **CR1 to AT3**:The alphanumeric codes (e.g., AT1,CR1) represent different
 experimental groups.The numbers represent FPKM
 values. **log2FoldChange**:It is used to measure the degree of difference
 in a gene's expression level between two conditions (e.g., CR vs.
 AT).**pvalue**:It is used to evaluate whether the observed difference in
 expression levels (i.e., the log2FoldChange) is statistically significant,
 rather than a chance result caused by random sampling
 error.**gene_biotype**: "gene_biotype" is a crucial field in
 genome annotation that describes the biological type of a gene or its
 transcript products.**gene_description**:The gene_description field
 typically contains knowledge compiled from existing scientific literature
 and databases, describing the known or predicted function of a gene. *
 Figure S6: **No-injection,dsGFP-24h,dsOR67c-24h**: Experimental group
 name. ## Code/software MEGA12: Molecular Evolutionary Genetics Analysis
 version 12 for adaptive and green computing Kumar S, Stecher G, Suleski M,
 Sanderford M, Sharma S, and Tamura K (2024)\ Molecular Biology and
 Evolution 41:1-9
 [https://magic-plus.novogene.com/#/](https://magic-plus.novogene.com/#/)
 ## Access information Other publicly accessible locations of the data: *
 NCBI database under accession number PRJNA1329347.