cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/t69cx-bst28
title: A413T cooverexpression

description: co overexpression strain UU1607/pJC3/pPM25  with A413T mutation in tsr (mutation made by Cathereine, strain and plasmids published. Sent from Heather Hodges (Kiessling lab)
description: <strong>Tilt Series Date:</strong> 2013-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew UU1607/pJC3/pPM25 overnight in 5mL TB + 3.8µl Cm + 5µl CarbDiluted back 1:50 into 200mL TB + 152µl Cm + 200µl Carb + 50µl 1M IPTG + 200µl Na-salicylateGrew ~2 hours to mid-log phaseSpun down 10' at 5,000 x gResuspended in 300µl lysis buffer + 0.1µl PMSF + 0.75 µl 1,10-phenanthrolineAdded 33.3µl (2mg/ml) fresh lysozyme solution (20mg/ml in 10mM Tris-HCl, pH 8.0)Incubated 45' at 37°CAdded 1.67µl 1M MgCl2, 0.2µl 1M CaCl2, 0.33µl DNase IIncubated 30' at 37°CSpun down 4000 g 5 min Resuspended in activity buffer (cold room) without glycerol</p>
  aba2013-08-30-30_slicer9544.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-30/rawdata/</p>A413tCoover0031.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-30/rawdata/A413tCoover0031.mrc"
        > <i class="download icon"></i> Download </a></p>

        A413tCoover0031.rec,  Reconstruction (Pixel Size 1.28 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-30/3dimage_30371/A413tCoover0031.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/20wp7-6xd11
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-44_slicer14252.jpg: Bdellovibrio bacteriovorus .  Filament between cells .  Note there are three cells in this tomogram
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-44/rawdata/</p>20130928_YWC034_10044.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-44/rawdata/20130928_YWC034_10044.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10044_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-44/3dimage_31434/20130928_YWC034_10044_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7wm2a-59c60
title: E.coli co-overexpression

description: E.coli co overexpression UU1607/pJC3/pPM25
description: <strong>Tilt Series Date:</strong> 2013-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain sent from Heather Hodges (Kiessling lab), parent strain and plasmids published from the Parkinson lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew UU1607/pJC3/pPM25 overnight in 5mL TB + 3.8µl Cm + 5µl CarbDiluted back 1:50 into 200mL TB + 152µl Cm + 200µl Carb + 50µl 1M IPTG + 200µl Na-salicylateGrew ~2 hours to mid-log phaseSpun down 10' at 5,000 x gResuspended in 300µl lysis buffer + 0.1µl PMSF + 0.75 µl 1,10-phenanthrolineAdded 33.3µl (2mg/ml) fresh lysozyme solution (20mg/ml in 10mM Tris-HCl, pH 8.0)Incubated 45' at 37°CAdded 1.67µl 1M MgCl2, 0.2µl 1M CaCl2, 0.33µl DNase IIncubated 30' at 37°CSpun down 4000 g 5 min Resuspended in activity buffer (cold room) without glycerol</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-17/rawdata/</p>cooverexpressionatlas4micronb0017.mrc,  Tilt Series (Pixel Size 0.28 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-17/rawdata/cooverexpressionatlas4micronb0017.mrc"
        > <i class="download icon"></i> Download </a></p>

        cooverexpressionatlas4micronb0017_part67_31.rec,  Reconstruction (Pixel Size 0.56 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-17/3dimage_28378/cooverexpressionatlas4micronb0017_part67_31.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/htz0z-we273
title: H. pylori 26695 / 20 nm gold-DNA

description: Grid box LR005
description: <strong>Tilt Series Date:</strong> 2014-01-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 3 days, OD600=0.4.  Spun cells down at 3000rpm for 5 minutes to concentrate before using; resuspended pellet.</p>
<strong>Sample Preparation:</strong> Combined 20ul cells with 10ul 10 nm gold fiducials (100ul gold and 25ul BSA) and 10ul 20 nm streptavidin gold-biotin DNA.  Grids frozen 7-20 minutes after combining DNA labeled gold with concentrated cells.  Vitrobot settings: 100% humidity, 22.5 C, -3 offset, 4.5 sec blot time, Ethane/propane mix.</p>
  ycw2014-01-21-27_slicer9891.jpg: H. pylori .  chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-01-21-27/rawdata/</p>20140121_LR005_30007.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-01-21-27/rawdata/20140121_LR005_30007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140121_LR005_30007_part121_24.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-01-21-27/3dimage_37929/20140121_LR005_30007_part121_24.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0fcyz-xtz98
title: H.pylori 26695 delta-PAI / AGS

description: Grid box: YWC098
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-15/rawdata/</p>20140401_YWC098_10015.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-15/rawdata/20140401_YWC098_10015.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140401_YWC098_10015_part81_1.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-15/3dimage_40723/20140401_YWC098_10015_part81_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a68d4-4fk47
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-17/rawdata/</p>20140331_YWC097_10017.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-17/rawdata/20140331_YWC097_10017.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10017_full.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-17/3dimage_40652/20140331_YWC097_10017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5trer-3aj93
title: H.pylori 26695 delta-cagH

description: Grid box: YWC090.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-02</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-02-1_slicer9876.jpg: Helicobacter pylori .  chemoreceptor ycw2014-03-02-1_slicer9877.jpg: Helicobacter pylori .  filament ycw2014-03-02-1_slicer9878.jpg: Helicobacter pylori .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-1/rawdata/</p>20140302_YWC090_10001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-1/rawdata/20140302_YWC090_10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140302_YWC090_10001_part131_11.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-1/3dimage_39462/20140302_YWC090_10001_part131_11.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s1s7v-h0e92
title: Hp 26695 delta-cagH / Hs.746T

description: grid box: YWC091
description: <strong>Tilt Series Date:</strong> 2014-02-17</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, culture to OD 0.6. AGS cells cultivated on QF Au finder grid for 2 days in DMEM + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-02-17-12_slicer17907.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-17-12/rawdata/</p>20140217_YWC091_10012.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-17-12/rawdata/20140217_YWC091_10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140217_YWC091_10012_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-17-12/3dimage_39072/20140217_YWC091_10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.jnpra6
title: Occurrence Download

description: A dataset containing 3338 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is NOAA Deep-Sea Coral Research and Technology Program",
 "Geometry POLYGON((-94.90907 25.82471,-91.11035 25.82471,-91.11035 28.62108,-94.90907 28.62108,-94.90907 25.82471))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "Year is 2017"
 ]
}

 The dataset includes 3338 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006274-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/x6qdb-23e14
title: H. pylori 26695 / AGS

description: Grid box YWC054
description: <strong>Tilt Series Date:</strong> 2013-11-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
  ycw2013-11-25-21_slicer10047.jpg: Helicobacter pylori .  flat vesicle .  chemoreceptor ycw2013-11-25-21_slicer17884.jpg: cag T4SS wi fi particle? ycw2013-11-25-21_slicer17885.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-21/rawdata/</p>20131125_YWC054_10021.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-21/rawdata/20131125_YWC054_10021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131125_YWC054_10021_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-21/3dimage_35056/20131125_YWC054_10021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yce7s-awq32
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC065
description: <strong>Tilt Series Date:</strong> 2014-05-12</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2014-05-12-1_slicer7295.jpg: unknown filament bundle ycw2014-05-12-1_slicer7296.jpg: flat vesicle ycw2014-05-12-1_slicer7298.jpg: pilli ycw2014-05-12-1_slicer7300.jpg: pilli ycw2014-05-12-1_slicer7302.jpg: external vesicle ycw2014-05-12-1_slicer7518.jpg: Myxococcus Xanthus  .  pilli .  7 nm thick
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-1/3dimage_42381/</p>20140512_YWC065_20001_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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        20140512_YWC065_20001.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
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