cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2014.245
title: COMPLETE GENOME SEQUENCE OF PORCINE EPIDEMIC DIARRHEA VIRUS IN VIETNAM

description: Porcine epidemic diarrhea (PED) is caused by PED virus (PEDV), an enveloped, positive-sense, single-stranded RNA virus belonging to the genus Alphacoronavirus, family Coronaviridae, order Nidovirales. PEDV first emerged in Vietnam in 2009. In this study, the complete genomes of three Vietnamese PEDV isolates were characterized. These three isolates were isolated from 3-day-old pigs experiencing diarrhea. Two isolates were from swine farms in the south, and the other was from North Vietnam. The whole genome sequences of these isolates are 28,035 nucleotides in length and have a genome characterization similar to that of other PEDV isolates with gene order 5’-ORF1a/1b-S-ORF3-E-M-N-3’. All three Vietnamese PEDV isolates share 99.8% and 99.6% sequence identity at the nucleotide and amino acid levels, respectively and have characteristics including deletion and insertion in the spike gene, namely, the insertion of 4 amino acids (GENQ) and 1 amino acid (N) at positions 56-59 and 140, respectively, and one deletion of 2 amino acids (DG) at positions 160 and 161. Phylogenetic analysis based on the whole genome revealed that the three Vietnamese PEDV isolates are grouped together with new variants from China that were responsible for an outbreak of PEDV from 2011 to 2012, and genetically distinct from US isolates and the classical PEDV variant. The results suggest that Vietnamese PEDV isolates are new variants as evidenced by their unique genetic composition of insertions and a deletion in the spike gene and share high genetic similarity with the new variants of Chinese PEDV isolates. This study provides a better understanding of the molecular characteristics of PEDV in Vietnam.
DOI: 10.5061/dryad.q5n39p4/3
title: PerSIMPERgroups
DOI: 10.5281/zenodo.3340578
title: finals_2019_07_165

description: Log and boot files of game 165
DOI: 10.5281/zenodo.3340579
title: finals_2019_07_165

description: Log and boot files of game 165
DOI: 10.5281/zenodo.3340582
title: finals_2019_07_166

description: Log and boot files of game 166
DOI: 10.5281/zenodo.3340583
title: finals_2019_07_166

description: Log and boot files of game 166
DOI: 10.5281/zenodo.3340584
title: finals_2019_07_167

description: Log and boot files of game 167
DOI: 10.5281/zenodo.3340585
title: finals_2019_07_167

description: Log and boot files of game 167
DOI: 10.5281/zenodo.3340586
title: finals_2019_07_168

description: Log and boot files of game 168
DOI: 10.5281/zenodo.3340587
title: finals_2019_07_168

description: Log and boot files of game 168
DOI: 10.14457/cu.the.2014.246
title: EXPERIMENTAL INFECTION OF FRANCISELLA NOATUNENSIS SUBSP. ORIENTALIS STRAIN VMCU-FNO131 IN RED TILAPIA (OREOCHROMIS SP.)

description: Since 2013, several outbreaks of an emerging disease characterized by multiple white granulomas in internal organs have occurred in cultured red tilapia (Oreochromis sp.) in some provinces in Thailand. The etiological agent of this disease in Thailand, however, has not yet been determined. The objective of the present study was to identify the causative agent of this infectious disease by fulfilling Koch’s postulates. Ten diseased red tilapia were collected from two affected farms for bacterial isolation, histological studies, and polymerase chain reaction (PCR) diagnosis for the suspected pathogen, Francisella sp.. All fish samples were positive for the Francisella genus-specific PCR. One bacterial strain, designated VMCU-FNO131, was successfully recovered from the kidney of diseased fish. Species identification of this strain was established by amplification and nucleotide sequencing of the 16S rRNA gene. BLAST analysis revealed 100 % identity to Francisella noatunensis subsp. orientalis strains available in the GenBank database. Subsequently, two groups of healthy red tilapia fingerlings were challenged with 1.08 x 103 CFU per fish and 1.08 x 105 CFU per fish by intraperitoneal injection. The accumulative mortality was 86.7 ± 23 % at 5 days post-injection (dpi). On the contrary, fish injected with 1.08 x 103 CFU per fish had died much slower, but the same accumulative mortality was observed at 16 dpi. Clinical signs and histopathological manifestations of typical granulomas were found in multiple organs of both the naturally and experimentally infected fish. Moreover, lethal dose 50 % (LD50) of VMCU-FNO131 in red tilapia was 2.88 x 105 CFU per fish at days 12 via intraperitoneal injection. In contrast, LD50 value could not calculate in immersion trial, and the dose 7 x 106 CFU mL-1 has just caused 25 % cumulative mortality within 21 days.
DOI: 10.14457/cu.the.2014.247
title: VIRULENCE PROPERTIES AND PATHOGENICITY OF FLAVOBACTERIUM COLUMNARE ISOLATES RECOVERED FROM DISEASED RED TILAPIA (OREOCHROMIS SP.) IN THAILAND

description: The causative agent of columnarsis disease, Flavobacterium columnare is a bacterial pathogen affecting many wild and farmed freshwater fishes, and subsequently resulting in significant economic losses globally. To date, various studies have been elucidated the pathogenesis of columnaris disease. However, comprehension of virulence factors and pathogenesis of F. columnare are still unclear. The objective of this study was to investigate pathogenicity of F. columnare isolates recovered from diseased red tilapia and some of their virulence-associated properties. Six specific primer pairs targeting each particular putative virulence gene were designed. The PCR results showed two distinct putative virulence gene profiles among 51 F. columnare isolates. However, it seems to be unrelated with pathogenicity in red tilapia. The LD50 of high, moderate and low virulence isolates were 6.10 log10CFU, 6.65 log10CFU and more than 8 log10CFU ml-1 of rearing water, respectively. The biofilm formation and adherent ability were significantly correlated with the virulence of F. columnare. The protease activity of F. columnare on skim milk agar was strong, however, lack of association with the levels of pathogenicity. Besides, high and moderate virulent isolates showed the high thickness of polysaccharide capsule when compare with low virulence isolate. In conclusion, adherences and thickness of polysaccharide capsule might play an important role in F. columnare virulence and infection in red tilapia.
DOI: 10.14457/cu.the.2014.248
title: PHARMACOGNOSTIC SPECIFICATIONS AND COUMARIN CONTENTS OF ALYXIA REINWARDTII AND EUPATORIUM STOECHADOSMUM

description: Alyxia reinwardtii Blume inner bark and Eupatorium stoechadosmum Hance whole plants have been used in traditional Thai medicine for a long time. This study was carried out to investigate the pharmacognostic specifications by qualitative and quantitative analyses as well as coumarin contents of A. reinwardtii inner bark and E. stoechadosmum whole plants. The samples were collected from 15 different sources throughout Thailand. Macroscopic and microscopic characteristics of two crude drugs were illustrated. The physicochemical properties of A. reinwardtii dried inner bark including loss on drying, total ash, acid- insoluble ash, water soluble extractive, ethanol soluble extractive, moisture and volatile oil contents were found to be 8.9±0.2, 7.7±0.1, 1.4±0.1, 16.5±0.1, 8.9±0.3, 11.9±0.2 and 0 % by weight, respectively. The physicochemical propoties of E. stoechadosmum whole plants were found to be 8.7±0.1, 9.5±0.3, 2.6±0.2, 27.8±0.4, 9.4±0.6, 13.2±0.3 and 0.14±0.0 % by weight, respectively. Coumarin in dichloromethane extract of A. reinwardtii dried inner bark and E. stoechadosmum dried whole plants were analyzed by thin layer chromatography (TLC) using silica gel 60 GF254 as stationary phase. Hexane and ethyl acetate (1:1) were used as mobile phase for A. reinwardtii. In case of E. stoechadosmum TLC procedure, first step developed with chloroform, and then with toluene, ethyl acetate and acetic acid (97:10:3). The coumarin contents were evaluated both TLC-densitometry performed using winCATS software and image analysis using imageJ software. The coumarin contents in A. reinwardtii crude drugs were found to be 0.77±0.04 and 0.75±0.01 g/100 g by those methods respectively. The coumarin contents in E. stoechadosmum crude drugs were found to be 0.44±0.02 and 0.45±0.04 g/100 g by those methods respectively. The coumarin contents between both methods were not statistically significant different (p&gt;0.05) by paired t-test. According to ICH guideline, TLC-densitometry and TLC image analysis using ImageJ software showed validity and reliability for coumarin quantitations in A. reinwardtii and E. stoechadosmum crude drugs. The chemical compositions of E. stoechadosmum volatile oils from 15 sources were analyzed by GC-MS. The result showed that the main component was binapacry (16.74±5.38%). E. stoechadosmum from 15 sources could be divided into two groups based on the dendrogram of volatile oil compositions constructed by UPGMA cluster analysis. This study provided scientistic evidences for identification, authentication, and quality control of A. reinwardtii and E. stoechadosmum crude drugs.
DOI: 10.14457/cu.the.2014.249
title: PHARMACOGNOSTIC SPECIFICATION AND VASICINE CONTENT OF ADHATODA VASICA LEAVES

description: Adhatoda vasica Nees or Malabar nut tree is a shrub which in Acanthaceae family. A. vasica is a famous herb that used for a long time mostly in Thai’s remedies. A. vasica leaves are used to treat fever and cough. For the good quality of herbal drugs, standardization is needed to be done. Leaf sample was collected from 12 different sources throughout Thailand. Whole plant of A. vasica was drawn by hand writing. In the microscopic method, A. vasica leaves powder and the cross section of mid rib was found calcium carbonate. Palisade ratio was 6.57 ± 0.56 whilst stomata number, stomata index, trichome number, trichome index and calcium carbonate number were 288.27 ± 3.70, 17.84 ± 0.83, 28.53 ± 7.63, 2.45 ± 0.53 and 36.50 ± 9.20, respectively. Physico-chemical method was shown that water content, loss on drying, total ash and acid insoluble ash were not more than 11, 9, 21 and 6 % by weight while ethanolic extract and water extract were not less than 4 and 22 % by weight. Thin layer chromatographic fingerprint of A. vasica leaf was performed using chloroform and methanol (9 : 1) as mobile phase and the developed TLC was photographed at 254 nm, 365 nm and strain with Dragendorff’s reagent. TLC quantitative analyses of samples using toluene : ethylacetate : diethylamine (5 : 2 : 3) as a mobile phase and analyzed by TLC-densitometry (CAMAG TLC scanner and winCATS) and TLC image analysis (ImageJ software). The calibration range were polynomial (1 – 5 µg/spot) and had R2 0.9998 and 0.9988, precision of method was in the range of 1.30 – 3.96 and 6.29 – 10.45, accuracy was 83.60 ± 1.09 and 85.32 ± 5.42, LOD and LOQ were 0.188, 0.067 and 0.570, 0.202 µg/spot and robustness of the method was 4.48 and 11.12 %, respectively. The total of vasicine in A. vasica leaves using TLC-densitometry and TLC image analysis was 0.130 ± 0.065 and 0.134 ± 0.061 g/100g of dried crude drug. The comparison of the total vasicine in both methods was not significant different (t = 1.796, P = 0.249). This study could be used for the standardization parameter of A. vasica leaves in Thailand and the development of this herbal drug species.
DOI: 10.14457/cu.the.2014.250
title: THE STRUCTURE OF COMMODITY TRADE BETWEEN THAILAND AND VIETNAM (2004-2013)

description: Using the quantitative approach as the main research methodology, this thesis analyzes trends and changes that have taken place in the structure of commodity trade between Thailand and Vietnam over the past decade (2004-2013). It will also provide several suggestions to promote Thailand-Vietnam commodity trade in the coming years. Findings show that along with the rapid growth in bilateral trade, trade composition between Thailand and Vietnam has changed positively towards increasing share of traded manufactures over the past decade. The thesis finds out that there has been a high share of trade in intermediate goods between the two countries, especially in Thailand’s exports while there has been a considerable growth in the share of final goods, especially capital goods in Vietnam’s exports. However, Vietnam has taken part only in assembling and processing stages with low value added and its exports to Thailand still depend heavily on comparative advantages over cheap labor and natural resources. Also, the thesis demonstrates that Vietnam’s exports have been less diversified as compared with Thailand. Although both countries enjoy a comparative advantage in primary products, there has been more medium and high technology products with high RCA index in Thailand’s exports as compared with Vietnam. This thesis therefore concludes that trade pattern between the two countries is a complementary trade relation. This thesis also argues that in order to promote Thailand-Vietnam commodity trade in the coming years, on Vietnam’s side, it should build appropriate policies on export-import orientation and export diversification. Vietnam should also enhance the competitiveness of domestic businesses as well as strengthen economic, trade and investment cooperation relations with Thailand.
DOI: 10.14457/cu.the.2014.251
title: The Study of Thailand's High School Policy, Curriculum and Pedagogy for Incorporation of Learning to Live Together (LTLT) Principles

description: It’s been almost twenty years since the concept of Learning To Live Together (LTLT); one of the four pillars of learning and a vitally important element for peaceful and successful living for the 21st century was endorsed by UNESCO for education for the twenty-first century. This research is an attempt to evaluate if LTLT or global citizenship skills have been successfully implemented through; strategic policy making; in tactical arrangements such as in curriculum planning and design, and finally through implementation in pedagogy in high schools in Thailand. Taught correctly these skills have a deep and profound influence on a young mind. The researcher will evaluate education policy and analyses the extent this is evident in the social studies/civics curriculum and how successful or not it is in the pedagogy. Using LTLT competencies: knowledge of others; empathy; acceptance; cultural sensitivity, communication skills, community involvement, teamwork, trust and political participation as a conceptual framework, the researcher will carry out case studies in two schools in Bangkok and ascertain if these competencies are being taught and more importantly how are they been taught. Transformational styles of pedagogy including student centred; problem solving; action/project based group work are examples of necessary teaching skills required for success. The researcher will challenge that even though LTLT or a global citizenship outlook is pledged in policy and curriculum, in practice it is poorly delivered because of a traditional teacher -centred pedagogy. Findings revealed that the core curriculum and social studies curricula do recognize some of the content of LTLT albeit not all of the competencies. The research also revealed a lack of connection between the expectation derived from government policy and the MOE, the social studies curriculum, school and teachers. Teaching practices were also inconsistent with the mandates which advocated a learner-centred focus and for students to take more initiatives in and become independent with their learning. The study exposed a distinctly teacher-centred style of pedagogy was being used whereby the focus was on the teacher controlling rather than on learner autonomy in the social studies classroom.
DOI: 10.14457/cu.the.2014.252
title: OPACITY AND DIVIDEND SIGNALING OF FUTURE PROFITABILITY

description: This thesis examines the relation between dividend changes and future profitability in respect of the degree of information asymmetry faced by U.S. firms during 1990 to 2013. As the information asymmetry is the theoretical incentive for firms to signal via dividends, firms with a high level of information asymmetry should be those that have the most incentives in taking such action. Contrary to this prediction, no conclusive evidence is found to support such hypothesis. Specifically, the results tend towards suggesting no evidence of dividend signaling. Additionally, the lack of the significance when testing the difference between high and low asymmetry firms greatly indicates that the difference in the level of asymmetry faced by firms has no impact on their decision to use dividend signaling. Hence, the findings of this thesis taken together suggest that dividends contain no information of future profitability, and plausibly that dividends are not the common means of signaling.
DOI: 10.14457/cu.the.2014.253
title: LEARNING ACTIVITY DESIGN TO ENHANCE PERSONAL HEALTH HABITS OF ELEMENTARY SCHOOL STUDENTS USING SELF-REGULATION CONCEPT: A CASE STUDY OF CAMBODIA

description: The purposes of the study were 1) to compare the mean scores of health practices on personal health according to Thai National Health Recommendation before and after experiment using self- regulation concept on the experimental and control groups 2) to compare the mean scores of health practices on personal health according to Thai National Health Recommendation before and after experiment using self-regulation concept between the experimental and control groups 3) to compare the mean scores of health practices on personal health according to Thai National Health Recommendation before experiment, after experiment and one month follow-up using self- regulation concept on the experimental group. This study was a quasi-experimental research, 30 students were classified as the experimental group and the other 30 students were the control group. Students in class 3A and 3B which were in grade three of Pi Thnou Primary School were selected as the experimental and control groups by purposive selection. Two instruments were used in this study. The first one was the 14 learning activity plans, which implemented within seven weeks. The learning activity management was conducted half and hour every Wednesday and Thursday. The second one was the practice questionnaires on personal health. The content validity (IOC) of the learning activity plans equaled to 0.90 and the reliability of the questionaire was 0.70. The mean scores, standard deviation and t-test were used for data analysis. F-test (one way ANOVA) was also used to compare the mean scores of pre-test, post-test and one month follow-up test of the experimental group. Finally, Scheffé was employed to test the differences of pair wise comparisons with the statistically significant at the .05 level. This study had been approved by National Ethic Committee for Health Research in Cambodia. The study findings were as follows: 1. After experiment, the mean scores of health practices of the experimental group (2.62) were found statistically higher significances than before experiment (2.48) at the .05 level. However the mean scores of health practices of the control group were found no statistically significant differences between before and after experiment. 2. Before experiment, the mean scores of health practices between the experimental group (2.48) and control group (2.46) were found no statistically significant differences at the .05 level. On the other hand, after experiment, the mean scores of health practices on personal health between the experimental group (2.62) and control group (2.50) were found statistically significant differences at the .05 level. 3. The mean scores of the health practices on personal health in the experimental group after experiment and one month follow-up were found statistically higher significances than before experiment. On the other hand, the mean scores between one month follow- up and after experiment were found no statistically significant differences at the .05 level.
DOI: 10.14457/cu.the.2014.254
title: DEVELOPMENT OF INTERNAL QUALITY ASSURANCE INDICATORS OF FACULTY OF EDUCATION IN CAMBODIA

description: The purpose of this research study was to develop a model of internal quality assurance indicators of faculty of education in Cambodia. The two specific objectives were 1) to construct a model of internal quality assurance indicators of faculty of education in Cambodia and 2) to validate the model of internal quality assurance indicators of the faculty. The sample included 3 respondent groups including 1) four experts of higher education and four stakeholders of faculty of education; 2) 800 teachers, staff, and students of faculty of education in the 2014 academic year; and 3) 11 associate deans of faculty of education and directors of regional teacher training centers in Cambodia. Three types of research instrument were used including the semi-structured interview form, questionnaire, and evaluation form. The descriptive statistics, content analysis, and confirmatory factor analysis were used in order to analyze the collected data. The results are concluded as follows: The model was composed of 6 dimensions and 22 sub-dimensions with 77 indicators. These six dimensions included 1) leadership; 2) mission, strategic planning, and finance; 3) educational programs; 4) quality of academic staff; 5) customers and support services; and 6) physical facilities. The model of internal quality assurance indicators of faculty of education in Cambodia fitted the empirical data and the six dimensions were important to ensure educational quality of faculty of education in Cambodia in that their factor loadings were statistically significant at the .01 level with the range from 0.583 to 0.936. Of the six dimensions, the most important to ensure educational quality was quality of academic staff, followed by educational programs; customers and support services; mission, strategic planning, and finance; physical facilities; and leadership which was of the least importance to ensure educational quality of the faculty. The model of internal quality assurance indicators were really suitable for faculty of education in Cambodia in that all the indicators strongly reflect the needs of the faculty and these indicators would be achieved at a high level when applied in the faculty.
DOI: 10.5061/dryad.q5n39p4/4
title: PerSIMPERmatrix
DOI: 10.5281/zenodo.3250923
title: A reference library for the identification of Canadian invertebrates: 1.5 million DNA barcodes, voucher specimens, and genomic samples

description: [This repository contains the source data for the manuscript "A reference library for the identification of Canadian invertebrates: 1.5 million DNA barcodes, voucher specimens, and genomic samples" by deWaard et al., 2019. BioRxiv]
description: {"references": ["deWaard et al., (2019) A reference library for the identification of Canadian invertebrates: 1.5 million DNA barcodes, voucher specimens, and genomic samples. BioRxiv."]}
DOI: 10.5281/zenodo.3340588
title: finals_2019_07_169

description: Log and boot files of game 169
DOI: 10.5281/zenodo.3340589
title: finals_2019_07_169

description: Log and boot files of game 169
DOI: 10.5281/zenodo.3340592
title: finals_2019_07_170

description: Log and boot files of game 170
DOI: 10.5281/zenodo.3340593
title: finals_2019_07_170

description: Log and boot files of game 170
DOI: 10.5281/zenodo.3340594
title: finals_2019_07_171

description: Log and boot files of game 171
DOI: 10.5281/zenodo.3340595
title: finals_2019_07_171

description: Log and boot files of game 171
DOI: 10.5281/zenodo.3340596
title: finals_2019_07_172

description: Log and boot files of game 172
DOI: 10.5281/zenodo.3340597
title: finals_2019_07_172

description: Log and boot files of game 172