cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.21430/m38lme8qib
title: Analyses of the specificity of CD4 T Cells during the primary immune response to influenza virus reveals dramatic MHC-linked asymmetries in reactivity to individual viral proteins

description: Influenza is a contagious, acute respiratory disease that is a major cause of morbidity and mortality throughout the world. CD4 T cells play an important role in the immune response to this pathogen through the secretion of antiviral cytokines, and by providing help to CD8 T cells and B cells to promote the development of immunological memory and neutralizing antibody responses. Despite these well-defined roles in the anti-influenza response, our understanding of CD4 T-cell diversity and specificity remains limited. In the study reported here, overlapping peptides representing 5 different influenza viral proteins were used in EliSpot assays to enumerate and identify the specificity of anti-influenza CD4 T cells directly ex vivo following infection of mice with influenza virus, using two strains that express unrelated MHC class II molecules. These experiments evaluated whether the reactivity of CD4 T cells generally tracked with particular influenza proteins, or whether MHC preferences were the predominant factor dictating anti-CD4 T-cell specificity in the primary immune response. We made the unexpected discovery that the distribution of CD4 T-cell specificities for different influenza proteins varied significantly depending on the single class II molecule expressed in vivo. In SJL mice, the majority of epitopes were specific for the HA protein, while the NP protein dominated the response in C57BL/10 mice. Given the diversity of human MHC class II molecules, these findings have important implications for the ability to rationally design a vaccine that will generate a specific CD4 T-cell immune response that is effective across diverse human populations.
DOI: 10.21430/m38oji5xi0
title: Decay of Memory T cells In Old Population

description: To expand our study of T cell memory repertoires in an aging population for which the repertoires may be decaying. These studies will provide data about the aging-specific dynamics of memory repertoires.
DOI: 10.21430/m38twq85kw
title: Pediatric Kidney Transplant Without Calcineurin Inhibitors (CN01)

description: The purpose of this study is to see the effect of using drugs other than calcineurin inhibitors to improve the rate of kidney transplant failure.Kidney transplantation can help children with end-stage kidney disease. However, it has been difficult to find treatment for donor graft rejection that does not have a lot of side effects. Researchers hope to find treatments (immunosuppressants) with fewer side effects. One approach is to avoid using calcineurin inhibitors and to try a new drug known as sirolimus instead. Another is to use steroids less often. This study will test whether using sirolimus, fewer steroid treatments, MMF, and certain antibodies will improve long-term graft survival in children receiving kidney transplants from living donors.
DOI: 10.21430/m38wxgbdts
title: Immunologic and genomic signatures of influenza vaccine response - 2010 (see companion studies SDY400, SDY404, SDY520)

description: Project 1: Immunologic and genomic signatures of influenza vaccine response.
DOI: 10.21430/m39ebhnryf
title: Human T Cell Profile

description: A multidimensional, quantitative analysis of human T cell compartmentalization and maintenance over six decades of life in blood, lymphoid, and mucosal tissues obtained from 56 individual organ donors.
DOI: 10.21430/m39rjdhjvb
title: Live Kidney Donor Study - Cross-Sectional and Historical Cohort Study (RELIVE-04)

description: The goal of this study is to determine the long-term risks of donor nephrectomy, compared to appropriate control subjects. This study built on information generated from RELIVE-01, a separate but related retrospective study. Through direct contact with previous living kidney donors this study examined the contribution of living kidney donation to the future development of hypertension, proteinuria, renal insufficiency or renal failure and anemia, the potential through these factors or others for increased cardiovascular risk for clinical events including myocardial infarction (MI), congestive heart failure (CHF), need for coronary artery bypass grafting (CABG) or percutaneous coronary angioplasty (PTCA) revascularization, and the impact of living kidney donation on quality of life (QOL) and financial status, compared to applicable control subjects and populations.
DOI: 10.21430/m39yln3479
title: Responses to Influenza Vaccination in Systemic Lupus Year 4 2008-2009

description: Compare the major components of the normal immune response to flu vaccination in SLE patients and control subjects in order to identify abnormalities in SLE group of immunocompromised individuals.
DOI: 10.21430/m3aa0xr32d
title: In-Depth Characterization of Immune Responses to Pneumovax Vaccination in Healthy Subjects

description: This study will measure the immune response to the Pneumovax vaccine The long-term goal is to develop improved vaccines to infectious diseases such as influenza. Blood will be collected from patients at several visits before and after vaccination. The blood will be used in a series of immunological tests to measure the strength and breadth of immune response. These assays may include T cell and B cell activation assays, microarray testing, Epimax, Epigen, and flow cytometry.
DOI: 10.21430/m3abjs44k6
title: Responses to Influenza Vaccination in Systemic Lupus Year 1 2005-2006

description: Compare the major components of the normal immune response to flu vaccination in SLE patients and control subjects in order to identify abnormalities in SLE group of immunocompromised individuals.
DOI: 10.21430/m3anetojec
title: Systems Biology Approach to Study Influenza Vaccine 2011-12 in Healthy Children (see companion studies SDY364, SDY368, SDY387)

description: To characterize the cellular and transcriptional signatures of response to Flu vaccine in healthy children.
DOI: 10.21430/m3aywx8not
title: Systems Biology Analysis of the response to Licensed Hepatitis B Vaccine (Engerix-B) (see companion study SDY690)

description: This project will contribute to the overall vision and goals of this U19 by analyzing the role of adjuvants in the humoral response to hep B vaccination in healthy individuals.
DOI: 10.21430/m3bar04lx3
title: SWHP03 A novel pig model of Helicobacter pylori infection demonstrating Th1 and cytotoxic T cell responses

description: This time-course study assesses systemic and local immune responses in pigs challenged with a European and African strain of Helicobacter pylori. Immunophenotype and gene expression was assessed in peripheral blood mononuclear cells every week over a course of 2 months. At the end of the study animals were euthanized and local immune responses as well as histological changes and bacterial burden were assessed.
DOI: 10.21430/m3bgahtyyo
title: Response of women during pregnancy to the inactivated influenza virus vaccine

description: Immunological changes play a physiological role in pregnancy, however they may also have consequences for vaccine response, which ultimately could impact maternal and fetal survival in the event of transmission of infectious diseases and/or bioterrorism.<br><br>The purpose of this study is to characterize the changes in immune response that occur during pregnancy.
DOI: 10.21430/m3cdx6tl4i
title: Systems Biology of 2008 Influenza Vaccination in Humans (See companion studies SDY61 2007 / SDY270 2009 / SDY271 Role for CaMKIV in the Regulation of Antibody Responses to Influenza Vaccine)

description: Using a systems biology approach to study innate and adaptive responses to influenza vaccination in humans during the 2008-2009 influenza season.
DOI: 10.21430/m3cjt3nct2
title: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2012 (See companion studies SDY311 2010 / SDY312 2009 / SDY314 2008 / SDY112 2011)

description: Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
DOI: 10.21430/m3d393v41d
title: Responses to Influenza Vaccination in Systemic Lupus Year 3 2007-2008

description: Compare the major components of the normal immune response to flu vaccination in SLE patients and control subjects in order to identify abnormalities in SLE group of immunocompromised individuals.
DOI: 10.21430/m3dlce81cy
title: Omega-3 Fatty Acids That Affect the Immune System in Kidney Transplant Patients (ID01)

description: The purpose of this study is to evaluate the effectiveness of nutritional supplements in increasing the amount of omega-3 fatty acids (and arginine) in the red blood cell membranes and plasma of kidney transplant patients, and, secondarily, to compare patient compliance. The long-term goal of this study is to develop low risk therapies that will allow improved and lasting survival of donor tissue with minimal suppression of the immune system.
DOI: 10.21430/m3dm0s6fpm
title: Live Kidney Donor Study (RELIVE-01)

description: The purpose of this study was to establish a multi-center kidney donor database containing renal failure, cardiovascular and overall mortality information for the complete cohorts of kidney donors who underwent living donor uninephrectomy.
DOI: 10.21430/m3du77m662
title: Aerosol vaccination in mice

description: A comparison between large and small droplet flu vaccination
DOI: 10.21430/m3dwbgxetn
title: Reactive oxygen species and TH2 responses to papain

description: The role of reactive oxygen species in the TH2 response to papain was investigated. The production of ROS by dendritic cells both in vivo and in vitro was examined as an inducer of TH2 differentiation and suppressor of TH1 differentiation. CD70 and IL-12 as effectors of ROS-mediated suppression of TH1 differentiation were investigated as well as the effect of ROS on IL-4-mediated TH2 response.
DOI: 10.21430/m3eaoxzb4o
title: Modeling Pulmonary Immunity: Evaluation of the Innate Immune Response to Pathogens 1) Francisella tularensis 2) Mycobacterium tuberculosis and 3) Influenza A virus in cynomolgus macaque

description: Alveolar macrophages from mice and macaques following infection with viral and bacterial pathogens were used to determine the similarities and differences to invading pulmonary pathogens
DOI: 10.21430/m3ej72rvrg
title: Systems Biology Approach to Analysis of 2010-11 TIV Fluzone Influenza Vaccine Response in Healthy Individuals (see companion studies SDY301, SDY296)

description: This study will measure the immune response to the influenza vaccine The long-term goal is to develop improved vaccines to infectious diseases such as influenza. Blood will be collected from patients at several visits before and after vaccination. The blood will be used in a series of immunological tests to measure the strength and breadth of immune response. These assays may include T cell and B cell activation assays, microarray testing, Epimax, Epigen, and flow cytometry.
DOI: 10.21430/m3emf3oms4
title: Delineate innate immune responses in populations at risk (the elderly and the immunosuppressed) that are different from the general population

description: We will assess the efficiency of the immune response of monocytes and dendritic cells in different populations (young adult and elderly) in response to stimulation with specific TLR ligands. We will focus separately on TLRs 3, 7, 8, 9, that recognize viral targets, TLRs 1, 2, 4, 6 (bacterial targets), and the effects of aging on MIF.<br><br>We will quantify TLR expression as well as efficiency of signaling pathways triggered by TLR engagement to identify mechanisms that contribute to the impairments of innate immunity associated with aging and immunosuppression.<br><br>Finally, we will assess the functional and genetic polymorphisms of MIF in the innate response to TLR stimulation.
DOI: 10.21430/m3eq20fg3w
title: Strategies to alleviate original antigenic sin

description: They explored strategies to overcome original antigenic sin responses in mice sequentially exposed to two closely related H1N1 influenza strains A/PR/8/34 and A/FM/1/47.
DOI: 10.21430/m3erwhdjeo
title: Simulation and Prediction of the Adaptive Immune Response and Quantification of Early and Adaptive Immune Response Kinetics to Influenza A Virus Infection

description: Modeling approaches were combined with experimental data to investigate innate and adaptive immune responses to IAV infection. Mathematical models developed describe the dynamic interactions between influenza virus, target cells, cytotoxic lymphocytes, and virus-specific IgG and IgM. In additon, a two-compartment model developed quantifies the effects of viral replication and adaptive immunity.
DOI: 10.21430/m3f8w7va7o
title: Systems Biology Study to Investigate Immune Correlates to Hepatitis B Vaccine Engerix-B comparing cellular responses and gene expression patterns between PBMCs (cell sorts) and whole blood samples (see companion studies SDY816 and SDY299)

description: This project will contribute to the overall vision and goals of this U19 by analyzing the role of adjuvants in the humoral response to hep B vaccination in healthy individuals.
DOI: 10.21430/m3fd1qtlqq
title: Responses to Influenza Vaccination in Systemic Lupus Year 2 2006-2007

description: Compare the major components of the normal immune response to flu vaccination in SLE patients and control subjects in order to identify abnormalities in SLE group of immunocompromised individuals.
DOI: 10.21430/m3fh0sa2w0
title: Systems Biology of 2007 Influenza Vaccination in Humans (See companion studies SDY269 2008 / SDY270 2009 / SDY271 Role for CaMKIV in the Regulation of Antibody Responses to Influenza Vaccine)

description: Using a systems biology approach to study innate and adaptive responses to influenza vaccination in humans during the 2007-2008 influenza season.
DOI: 10.21430/m3fjj9g9zz
title: Healthy Human DC and monocyte subsets transcriptional regulations in response to Fluzone 2010-2011 and pneumococcal vaccinations

description: The described experiments in this study were designed to deconvolute the molecular signature observed in the whole blood of healthy subsets at early time points following the administration of Fluzone 2010-2011 vaccines. RNA-seq data generated from sorted purified cell populations, including mDC and monocyte subsets will permit us to reveal distinct roles that DC and monocyte subsets play in eliciting immune responses to vaccines against flu in healthy adults using system biology approaches.
DOI: 10.21430/m3g310zbsi
title: RELIVE Informed Consent Study (RELIVE-03)

description: The goal of this study was to study informed consent by the living donor. This study used surveys to evaluate the understanding of risk and psychological pressure that living organ donors felt when making the decision to donate. It compared participants' answers across geographic, racial and socio-economic backgrounds.
DOI: 10.21430/m3g33vvu77
title: Immune Response to Varicella Vaccination in Subjects with Atopic Dermatitis Compared to Nonatopic Controls (VAR 05)

description: Young children have the highest prevalence of atopic dermatitis (AD) and are at significant risk complications from vaccinia. As a surrogate, we believe investigating the immune response to varicella vaccination will yield important information about alterations in the immune response to live virus vaccines subjects with AD. Evaluation of 30 analyzable children with AD and 30 analyzable nonatopic controls is planned. Subjects immunized with varicella vaccine will have blood drawn for laboratory evaluations 3 weeks after immunization. The immune response to varicella will be assessed by measuring the proportion of varicella-zoster virus (VZV)-specific T cells, perforin production, and VZV-specific antibody titers. Because of the and systemic T helper cell 2 (Th2) polarization of subjects with AD, an altered response to varicella vaccination expected. Specifically, it is expected that AD subjects will exhibit decreased cell-mediated responses, but exhibit normal to increased humoral responses as evidenced by production of VZV-specific antibodies.
DOI: 10.21430/m3g81pa5g7
title: ADVN Biomarker Registry Study: CMI/Ab-Vaccinia Substudy

description: To measure total and specific antibody titers and T cell responses in a sample of subjects who experienced eczema vaccinatum (EV) and a group of subjects who did not suffer from EV (normal controls or healthy AD subjects).
DOI: 10.21430/m3gcsv0kae
title: Cooperation between DCs and basophils in TH2 response to papain

description: To investigate the contribution of dendritic cells and basophils to TH2 differentiation, induction with OVA and papain was used to investigate signalling in both in vivo and in vitro systems.
DOI: 10.21430/m3gtk55vev
title: Characterization of in vitro Stimulated B Cells from Human Subjects

description: During the human B cell (Bc) recall response, rapid cell division results in multiple Bc subpopulations. RNA microarray and functional analyses showed that proliferating CD27lo cells are a transient pre-plasmablast population, expressing genes associated with Bc receptor editing. Undivided cells had an active transcriptional program of non-ASC B cell functions, including cytokine secretion and costimulation, suggesting a link between innate and adaptive Bc responses. Transcriptome analysis suggested a gene regulatory network for CD27lo and CD27hi Bc differentiation.
DOI: 10.21430/m3h9n1sflo
title: Systems Biology of 2009 Influenza Vaccination in Humans (See companion studies SDY61 2007 / SDY269 2008 / SDY271 Role for CaMKIV in the Regulation of Antibody Responses to Influenza Vaccine)

description: Using a systems biology approach to study innate and adaptive responses to influenza vaccination in humans during the 2009-2010 influenza season.
DOI: 10.21430/m3h9vmi0df
title: Modeling Pulmonary Immunity: Evaluation of the Innate Immune Response to Pathogens 1) Francisella tularensis 2) Mycobacterium tuberculosis and 3) Influenza A virus in mice (C57BL/6 and BALB/c)

description: Alveolar macrophages from mice and macaques following infection with viral and bacterial pathogens were used to determine the similarities and differences to invading pulmonary pathogens
DOI: 10.21430/m3hhufk741
title: Host Differences in influenza-specific CD4 T cell and B cell responses are modulated by viral strain and route of immunization

description: The antibody response to influenza infection is largely dependent on CD4 T cell help for B cells. Cognate signals and secreted factors provided by CD4 T cells drive B cell activation and regulate antibody isotype switching for optimal antiviral activity. Recently, we analyzed HLA-DR1 transgenic (DR1) mice and C57BL/10 (B10) mice after infection with influenza virus A/New Caledonia/20/99 (NC) and defined epitopes recognized by virus-specific CD4 T cells. Using this information in the current study, we demonstrate that the pattern of secretion of IL-2, IFN-g, and IL-4 by CD4 T cells activated by NC infection is largely independent of epitope specificity and the magnitude of the epitope-specific response. Interestingly, however, the characteristics of the virus-specific CD4 T cell and the B cell response to NC infection differed in DR1 and B10 mice. The response in B10 mice featured predominantly IFN-g-secreting CD4 T cells and strong IgG2b/IgG2c production. In contrast, in DR1 mice most CD4 T cells secreted IL-2 and IgG production was IgG1-biased. Infection of DR1 mice with influenza PR8 generated a response that was comparable to that in B10 mice, with predominantly IFN-g-secreting CD4 T cells and greater numbers of IgG2c than IgG1 antibody-secreting cells. The response to intramuscular vaccination with inactivated NC was similar in DR1 and B10 mice; the majority of CD4 T cells secreted IL-2 and most IgG antibody-secreting cells produced IgG2b or IgG2c. Our findings identify inherent host influences on characteristics of the virus-specific CD4 T cell and B cell responses that are restricted to the lung environment. Furthermore, we show that these host influences are substantially modulated by the type of infecting virus via the early induction of innate factors. Our findings emphasize the importance of immunization strategy for demonstrating inherent host differences in CD4 T cell and B cell responses.
DOI: 10.21430/m3hruxrofp
title: Steroid Withdrawal in Pediatric Kidney Transplant Recipients (SW01)

description: The purpose of this study is to examine the effects of withdrawing steroids on graft rejection and kidney functions in kidney transplant recipients between the ages of 0 and 20 years (prior to their 21st birthday). Graft survival has improved in recent years in children with kidney transplants. One bad side effect of steroid maintenance therapy has been growth retardation. Doctors believe steroids might be safely withdrawn in patients that are receiving other maintenance therapies. If steroids are removed, children might catch up in their growth and also might have fewer side effects of other kinds. This study evaluates whether steroid therapy can be withdrawn in a way that does not increase graft rejection.
DOI: 10.21430/m3i0jl7kuz
title: Asthma Control Evaluation (ACE): A Biomarker-Based Approach to Improving Asthma Control and Mechanistic Studies (ICAC-02)

description: The purpose of ICAC-01 is to determine whether an asthma treatment strategy that measures exhaled nitric oxide (eNO) to indicate disease progression is more effective in treating asthma symptoms when combined with existing asthma treatment guidelines than treatment using the guidelines alone.
DOI: 10.21430/m3ibwcgi97
title: Orban ITN012AI: Evaluation of Diabetes Vaccine IBC-VSO1 in Newly Diagnosed Diabetics

description: IBC-VSO1 vaccine, a synthetic metabolically inactive form of insulin is being evaluated as means of preventing pancreatic beta-cell destruction in newly diagnosed type 1 diabetes patients.
DOI: 10.21430/m3iy2gjyv6
title: Immune Memory Boosting Dose of Rapamycin Impairs Macrophage Vesicle Acidification and Curtails Glycolysis in Effector CD8 Cells, Impairing Defense against Acute Infections

description: Direct mammalian target of rapamycin (Rapa) complex 1 inhibition by short-term low-dose Rapa treatment has recently been shown to improve CD8 T cell immunological memory. Whereas these studies focused on memory development, the impact of low-dose Rapa on the primary immune response, particularly as it relates to functional effector immunity, is far less clear. In this study, we investigated the impact of acute Rapa treatment on immune effector cell function during the primary immune response to several acute infections. We found that functional CD8 T cell and macrophage responses to both viral and intracellular bacterial pathogens were depressed in mice in vivo and in humans to phorbol ester and calcium ionophore stimulation in vitro in the face of low-dose Rapa treatment. Mechanistically, the CD8 defect was linked to impaired glycolytic switch in stimulated naive cells and the reduced formation of short-lived effector cells. Therefore, more than one cell type required for a protective effector immune response is impaired by Rapa in both mice and humans, at the dose shown to improve immune memory and extend lifespan. This urges caution with regard to the relative therapeutic costs and benefits of Rapa treatment as means to improve immune memory.
DOI: 10.21430/m3j0s60rl6
title: Age-associated alterations in CD8a+ dendritic cells impair CD8

description: Age-associated decline in immunity to infection has been documented
DOI: 10.21430/m3j22zozm9
title: ADVN Biomarker Registry Study

description: The proposed Registry is a database with a minimum of 1,000 subjects to improve scientific understanding of the increased risk of complications after exposure to the smallpox vaccine for people with atopic dermatitis.