DOI: 10.5061/dryad.2jh32
title: Data from: Selection on outlier loci and their association with adaptive phenotypes in Littorina saxatilis contact zones

description: A fundamental issue in speciation research is to evaluate phenotypic
 variation and the genomics driving the evolution of reproductive isolation
 between sister taxa. Above all, hybrid zones are excellent study systems
 for researchers to examine the association of genetic differentiation,
 phenotypic variation and the strength of selection. We investigated two
 contact zones in the marine gastropod Littorina saxatilis and utilized
 landmark-based geometric morphometric analysis together with amplified
 fragment length polymorphism (AFLP) markers to assess phenotypic and
 genomic divergence between ecotypes under divergent selection. From
 genetic markers, we calculated the cline width, linkage disequilibrium and
 the average effective selection on a locus. Additionally, we conducted an
 association analysis linking the outlier loci and phenotypic variation
 between ecotypes and show that a proportion of outlier loci are associated
 with key adaptive phenotypic traits.
description: LittorinaSaxatilis_AFLPsThe file LittorinaSaxatilis_AFLPs.txt correspond to the AFLP dataset used in this manuscript where we studied a hybrid zone of the marine gastropod Littorina saxatilis. It contains AFLP genotypes (1 for presence, 0 for absence, 9 for missing data). The first 4 columns contain ISLAND, ECOTYPE, POPULATION and INDIVIDUAL information. The rest of the columns represent the genotypes (0,1) of 1167 AFLP loci studied. The first row contains the loci names according to the analysis of outlier loci. The second row contains the loci names according to their primer combination and size in base pairs. The list of outlier loci detected for each island (RAMSÖ, RAMSHOLMEN)in this study is available in the file LittorinaSaxatilis_AFLPs_list_outliers.txt. The landmark data from the geometric morphometric analysis is available in the file LittorinaSaxatilis_LandmarkData.txt. The names of the INDIVIDUALS are the same as the names in the LittorinaSaxatilis_AFLPs.txt file. We studied E (exposed) and S (sheltered) Littorina saxatilis ecotypes from 2 islands from shores of Sweden. In each island we sampled one transect along the L. saxatilis hybrid zone which contained two parental populations (2S, 2E) at +27m and -27m from the hybrid population, two populations (1S, 1E) at +9m and -9m from the hybrid population and one hybrid population (H). LOCALITY: RAMSÖ, RAMSHOLMEN ECOTYPE: E (exposed), S (sheltered), H (hybrid) POPULATION: 2S, 1S, H, 1E, 2E INDIVIDUAL: This is the individual code. The sampling, AFLP analysis and genotyping have been performed by Johan Hollander (johan.hollander@biol.lu.se).LittorinaSaxatilis_AFLPs_list_outliersThe list of outlier loci detected for each island (RAMSÖ, RAMSHOLMEN)in this study is available in the file LittorinaSaxatilis_AFLPs_list_outliers.txt.LittorinaSaxatilis_LandmarkDataThe landmark data from the geometric morphometric analysis is available in the file LittorinaSaxatilis_LandmarkData.txt. The names of the INDIVIDUALS are the same as the names in the LittorinaSaxatilis_AFLPs.txt file.