DOI: 10.25349/d9q905
title: Data For: Diet changes thermal acclimation capacity, but not acclimation rate in a marine ectotherm (Girella nigricans) during warming

description: Global climate change is increasing thermal variability in coastal marine
 environments and the frequency, intensity, and duration of marine
 heatwaves. At the same time, food availability and quality are being
 altered by anthropogenic environmental changes. Marine ectotherms often
 cope with changes in temperature through physiological acclimation, which
 can take several weeks and is a nutritionally demanding process.
 Here, we tested the hypothesis that different
 ecologically relevant diets (omnivorous, herbivorous,
 carnivorous) impact thermal acclimation rate and capacity, using a
 temperate omnivorous fish as a model (opaleye; Girella
 nigricans). We measured acute thermal performance curves for
 maximum heart rate because cardiac function has been observed to set upper
 thermal limits in ectotherms. Opaleye acclimated rapidly after
 raising water temperatures, but their thermal limits and acclimation rate
 were not affected by their diet. However, the fish’s acclimation capacity
 for maximum heart rate was sensitive to diet, with fish in the herbivorous
 treatment displaying the smallest change in heart rate throughout
 acclimation. Mechanistically, ventricle fatty acid composition differed
 with diet treatment and was related to cardiac performance in ways
 consistent with homoviscous adaptation. Our results suggest that
 diet is an important, but often overlooked, determinant of thermal
 performance in ectotherms on environmentally relevant timescales.
description: R code included. Data files are described in the README.md and
 open in any application that supports CSV files.
description: # Data For: Diet Changes Thermal Acclimation Capacity, but not Acclimation
 Rate in a Marine Ectotherm (Girella Nigricans) During Warming
 [https://doi.org/10.25349/D9Q905](https://doi.org/10.25349/D9Q905) ##
 GENERAL INFORMATION 1\. Author Information A. Principal Investigator
 Contact Information Name: Dr. Erika Eliason Institution: University of
 California, Santa Barbara Email:
 [eliason@ucsb.edu](mailto:eliason@ucsb.edu) B. Corresponding Author
 Contact Information Name: Emily Hardison Institution: University of
 California, Santa Barbara Email:
 [emilyhardison@ucsb.edu](mailto:emilyhardison@ucsb.edu) 2\. Date range of
 data collection (single date, range, approximate date): 2021 - 2022 3\.
 Geographic location of data collection: Santa Barbara, California, USA 4\.
 Information about funding sources that supported the collection of the
 data:  This work was supported by a Hellman Family Faculty Fellowship and
 the University of California, Santa Barbara. Additional funding for E.A.H.
 was provided by National Science Foundation Graduate Research Fellowship
 and the Santa Barbara Coastal Long Term Ecological Research Project.
 Additional support for G.S. was provided by the Tri-County Blood Bank
 Post-Doctoral Fellowship. We thank the Santa Barbara Coastal Long Term
 Ecological Research Project under the National Science Foundation
 Cooperative Agreement #OCE-1831937. ## SHARING/ACCESS INFORMATION 1\.
 Licenses/restrictions placed on the data: N/A 2\. Links to publications
 that cite or use the data: N/A 3\. Links to other publicly accessible
 locations of the data: N/A 4\. Links/relationships to ancillary data sets:
 N/A 5\. Was data derived from another source? no ## DATA & FILE
 OVERVIEW 1\. File List:  Hardison2022_ABT_data.csv - contains data from
 Hot Arrhenius breakpoint test on the heart
 Hardison2022_ventricle_FattyAcid.csv - contains ventricle fatty acid
 composition data and relevant cardiac performance metrics 
 hardison2022_abt_TPC_data.csv - contains data to generate figure 1 (TPC
 for fhmax).  Hardison2022_figure4_values.csv - contains mean values of
 peak fhmax from the literature and this study for generating figure 4 of
 the manuscript Hardison2022_coldABT_data.csv - contains data from Cold
 Arrhenius breakpoint test on the heart ## METHODOLOGICAL INFORMATION 1\.
 Description of methods used for collection/generation/processing of data: 
 Please see main manuscript for detailed information regarding the methods
 used to collect/analyze this data  2\. People involved with sample
 collection, processing, analysis and/or submission: Emily Hardison, Gail
 Schwieterman, Erika Eliason ## DATA-SPECIFIC INFORMATION DATA-SPECIFIC
 INFORMATION FOR: hardison2022_abt_TPC_data.csv 1\. Number of variables: 8
 2\. Number of cases/rows: 1130 3\. Variable List: * fish_id = Unique
 identifier assigned to each fish's data.  Note that this identifier
 is unique to this dataset.  * replicate_number = the study was conducted
 over 5 replicates. The replicate each fish was tested in is indicated in
 this column. * diet_treatment = Diet treatment (omnivorous = Omni or
 carnivorous = Carn, herbivorous = Herb) * bm_g = body mass of the fish in
 grams * timepoint = timepoint of test (either day 0, 1, 3, 7, 14), where
 day 0 was after 2 weeks of acclimation to 12°C and day 14 was after 2
 weeks of acclimation to 20°C * bm_g = body mass of the fish in grams *
 acute_temperature = the temperature (°C) corresponding to the fHmax taken
 at each ~degree interval during the ABT test  * fh_bpm = Maximum heart
 rate in beats per minute at each acute temperature increase * t_arr =
 Temperature (in °C) corresponding to onset of cardiac arrhythmia  
 DATA-SPECIFIC INFORMATION FOR: Hardison2022_ABT_data.csv  1\. Number of
 variables: 10 2\. Number of cases/rows: 153 3\. Variable List: * row_id =
 Unique identifier assigned to each fish's data.  Note that this
 identifier is unique to this dataset.  * Timepoint = timepoint of test
 (either day 0, 1, 3, 7, 14), where day 0 was after 2 weeks of acclimation
 to 12°C and all other timepoints were after ramping to 20°C *
 Replicate_number = the study was conducted over 5 replicates. The
 replicate each fish was tested in is indicated in this column. *
 diet_treatment = Diet treatment (omnivorous = Omni or carnivorous = Carn,
 herbivorous = Herb) * bm_g = body mass of the fish in grams * t_abt =
 Arrhenius breakpoint temperature in °C * t_peak = Temperature (in °C)
 corresponding to maximum overall heart rate * t_arr = Temperature (in °C)
 corresponding to onset of cardiac arrhythmia * peak_fhmax = Maximum
 overall heart rate in beats per minute * liver_mass_g = liver mass of the
 fish in grams   DATA-SPECIFIC INFORMATION FOR:
 Hardison2022_figure4_values.csv 1\. Number of variables: 5 2\. Number of
 cases/rows: 1130 3\. Variable List: * data_source = where the data came
 from (either a quick cite from the literature, direct from the harbor, or
 from treatment level means in the current study) * acclimation_temperature
 = acclimation temperature in °C * peak_fhmax = Maximum overall heart rate
 in beats per minute * se_peak_fhmax = standard error for overall heart
 rate in beats per minute * diet_treatment = Diet treatment (omnivorous =
 Omni or carnivorous = Carn, herbivorous = Herb) - only relevant to
 treatment level means in the current study DATA-SPECIFIC INFORMATION FOR:
 Hardison2022_ventricle_FattyAcid.csv   1\. Number of variables: 53 2\.
 Number of cases/rows: 54 3\. Variable List: * row_id = Unique identifier
 assigned to each fish's data.  Note that this identifier is unique to
 this dataset.  * diet_treatment  =Diet treatment (omnivorous = Omni or
 carnivorous = Carn, herbivorous = Herb) * timepoint  = timepoint of test
 (either day 0 or 14 for this dataset), where day 0 was after 2 weeks of
 acclimation to 12°C and day 14 was after ramping to 20°C for two weeks *
 acclimation_temp = Acclimation temperature in °C (12 or 20°C) - 12_0 =
 total amount of fatty acid with chain length 12 and 0 double bonds in
 pmol/mg tissue -  14_0 = total amount of fatty acid with chain length 14
 and 0 double bonds in pmol/mg tissue - 15_0 = total amount of fatty acid
 with chain length 15 and 0 double bonds in pmol/mg tissue -  16_0 = total
 amount of fatty acid with chain length 16 and 0 double bonds in pmol/mg
 tissue -  16_1= total amount of fatty acid with chain length 16 and 1
 double bonds in pmol/mg tissue - 17_0 = total amount of fatty acid with
 chain length 17 and 0 double bonds in pmol/mg tissue -  17_1= total amount
 of fatty acid with chain length 17 and 1 double bonds in pmol/mg tissue -
  18_0 = total amount of fatty acid with chain length 18 and 0 double bonds
 in pmol/mg tissue -  18_1= total amount of fatty acid with chain length 18
 and 1 double bonds in pmol/mg tissue -  18_2 = total amount of fatty acid
 with chain length 18 and 2 double bonds in pmol/mg tissue -  18_3_n3 =
 total amount of fatty acid with chain length 18 and 3 double bonds (n3
 double bond included) in pmol/mg tissue - 18_3_n6 = total amount of fatty
 acid with chain length 18 and 3 double bonds (n6 double bond included) in
 pmol/mg tissue - 18_4 = total amount of fatty acid with chain length 18
 and 4 double bonds in pmol/mg tissue - 20_0 = total amount of fatty acid
 with chain length 20 and 0 double bonds in pmol/mg tissue - 20_1 = total
 amount of fatty acid with chain length 20 and 1 double bonds in pmol/mg
 tissue - 20_2 = total amount of fatty acid with chain length 20 and 2
 double bonds in pmol/mg tissue - 20_3_n3 = total amount of fatty acid with
 chain length 20 and 3 double bonds (n3 double bond included) in pmol/mg
 tissue - 20_3_n6 = total amount of fatty acid with chain length 20 and 3
 double bonds (n6 double bond included) in pmol/mg tissue - 20_3_n9 = total
 amount of fatty acid with chain length 20 and 3 double bonds (n9 double
 bond included) in pmol/mg tissue - 20_4 = total amount of fatty acid with
 chain length 20 and 4 double bonds in pmol/mg tissue - 20_5 = total amount
 of fatty acid with chain length 20 and 5 double bonds in pmol/mg tissue -
 22_0 = total amount of fatty acid with chain length 22 and 0 double bonds
 in pmol/mg tissue - 22_1 = total amount of fatty acid with chain length 22
 and 1 double bonds in pmol/mg tissue - 22_2 = total amount of fatty acid
 with chain length 22 and 2 double bonds in pmol/mg tissue - 22_3 = total
 amount of fatty acid with chain length 22 and 3 double bonds in pmol/mg
 tissue - 22_4 = total amount of fatty acid with chain length 22 and 4
 double bonds in pmol/mg tissue - 22_5_n3 = total amount of fatty acid with
 chain length 22 and 5 double bonds (n3 double bond included) in pmol/mg
 tissue - 22_5_n6 = total amount of fatty acid with chain length 22 and 5
 double bonds (n6 double bond included) in pmol/mg tissue - 22_6 = total
 amount of fatty acid with chain length 22 and 6 double bonds in pmol/mg
 tissue - 23_0 = total amount of fatty acid with chain length 23 and 0
 double bonds in pmol/mg tissue - 24_0 = total amount of fatty acid with
 chain length 24 and 0 double bonds in pmol/mg tissue - 24_1 = total amount
 of fatty acid with chain length 24 and 1 double bonds in pmol/mg tissue -
 26_0 = total amount of fatty acid with chain length 26 and 0 double bonds
 in pmol/mg tissue - total_fa  = total fatty acid composition in pmol/mg
 tissue - SFA = total saturated fatty acid composition in pmol/mg tissue -
 MUFA = total monounsaturated fatty acid composition in pmol/mg tissue -
 PUFA = total polyunsaturated fatty acid composition in pmol/mg tissue -
 HUFA  = total polyunsaturated fatty acid composition in pmol/mg tissue -
 DBI =  Double Bond Index - chain_length_index  = Average chain length of
 fatty acids - omega_3 = total omega-3 fatty acid composition in pmol/mg
 tissue - omega_6 = total omega-6 fatty acid composition in pmol/mg tissue
 - n3_n6_ratio = ratio of omega 3 to omega 6 fatty acids - peak_fhmax =
 Maximum overall heart rate in beats per minute - t_peak = Temperature (in
 °C) corresponding to maximum overall heart rate - t_arr = Temperature (in
 °C) corresponding to onset of cardiac arrhythmia - ventricle_mass =
 ventricle mass of the fish in grams - fhmax_20 = maximum heart rate at
 20°C in 20°C acclimated fish - fhmax_12 = maximum heart rate at 12°C in
 12°C acclimated fish. NA indicates fish where the test started at
 &gt;12.7°C and therefore, an estimate of fhmax at 12°C was not obtained
 DATA-SPECIFIC INFORMATION FOR: Hardison2021_coldABT_data.csv  1\. Number
 of variables: 6 2\. Number of cases/rows: 19 3\. Variable List: * row_id =
 Unique identifier assigned to each fish's data.  Note that this
 identifier is unique to this dataset.  * Replicate_number = the study was
 conducted over 5 replicates. The replicate each fish was tested in is
 indicated in this column. * diet_treatment = Diet treatment (omnivorous =
 Omni or carnivorous = Carn, herbivorous = Herb) * bm_g = body mass of the
 fish in grams * cold_tarr = Temperature (in °C) corresponding to onset of
 cardiac arrhythmia * liver_mass_g = liver mass of the fish in grams