cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4584271.v2
title: Midbody localization of vinexin recruits rhotekin to facilitate cytokinetic abscission

description: Vinexin is a SH3 domain-containing adaptor protein that has diverse roles in cell adhesion, signal transduction, gene regulation and stress granule assembly. In this study, we found that vinexin localizes at the midbody during cell division and facilitates cytokinesis. Knockdown of vinexin in HeLa cells delayed the mitotic cell cycle progression and increased the time of cell abscission and the failure to resolve the cytoplasmic bridge. Midbody-localized vinexin is essential for recruiting rhotekin to this structure for cytokinesis because overexpression of a vinexin mutant without a rhotekin-binding motif or knockdown of rhotekin also impaired cytokinetic abscission and increased the number of cells arrested at the midbody stage. Aberrant expression of vinexin and rhotekin in various cancers has been implicated to promote metastasis because of their functions in cell adhesion and signaling. Our findings reveal a novel role of vinexin and rhotekin in cytokinetic abscission and provide another perspective of how both molecules may affect oncogenic transformation via this fundamental cell cycle process.
DOI: 10.6084/m9.figshare.4584133
title: The effects of dehydroepiandrosterone on sexual function: a systematic review

description: <b>Objective:</b> Faced with the growing interest about the action of dehydroepiandrosterone (DHEA) and its benefits, as well as the negative impacts that sexual dysfunctions have on people’s quality of life, this systematic review was undertaken with the objective of evaluating the effect of DHEA use on aspects of sexual function. <b>Method:</b> An electronic search was conducted in the databases of PubMed, ISI Web of Science and Virtual Health Library (VHL) combining the terms 'DHEA treatment' and 'DHEA use' with terms such as 'sexual dysfunction', 'sexual frequency' and 'libido'. No limits on time and language were imposed. Clinical studies were considered eligible where individuals for any reason made use of DHEA and if they had any aspect of sexual function assessed. Preclinical studies and systematic reviews were considered ineligible. <b>Results:</b> The search identified 183 references and 38 were considered eligible. DHEA improved aspects such as sexual interest, lubrication, pain, arousal, orgasm and sexual frequency. Its effect was better in populations with sexual dysfunction, especially in perimenopausal and postmenopausal women. <b>Conclusion:</b> Considering the studies currently published, DHEA is effective in improving several aspects of sexual function, but this effect did not reach all the populations studied.
DOI: 10.6084/m9.figshare.4584271.v1
title: Midbody localization of Vinexin recruits Rhotekin to facilitate cytokinetic abscission

description: Vinexin is a SH3 domain-containing adaptor protein that has diverse roles in cell adhesion, signal transduction, gene regulation and stress granule assembly. In this study, we found that vinexin localizes at the midbody during cell division and facilitates cytokinesis. Knockdown of vinexin in HeLa cells delayed the mitotic cell cycle progression and increased the time of cell abscission and the failure to resolve the cytoplasmic bridge. Midbody-localized vinexin is essential for recruiting rhotekin to this structure for cytokinesis because overexpression of a vinexin mutant without a rhotekin-binding motif or knockdown of rhotekin also impaired cytokinetic abscission and increased the number of cells arrested at the midbody stage. Aberrant expression of vinexin and rhotekin in various cancers has been implicated to promote metastasis because of their functions in cell adhesion and signaling. Our findings reveal a novel role of vinexin and rhotekin in cytokinetic abscission and provide another perspective of how both molecules may affect oncogenic transformation via this fundamental cell cycle process.
DOI: 10.6084/m9.figshare.4584133.v1
title: The effects of dehydroepiandrosterone on sexual function: a systematic review

description: <b>Objective:</b> Faced with the growing interest about the action of dehydroepiandrosterone (DHEA) and its benefits, as well as the negative impacts that sexual dysfunctions have on people’s quality of life, this systematic review was undertaken with the objective of evaluating the effect of DHEA use on aspects of sexual function. <b>Method:</b> An electronic search was conducted in the databases of PubMed, ISI Web of Science and Virtual Health Library (VHL) combining the terms 'DHEA treatment' and 'DHEA use' with terms such as 'sexual dysfunction', 'sexual frequency' and 'libido'. No limits on time and language were imposed. Clinical studies were considered eligible where individuals for any reason made use of DHEA and if they had any aspect of sexual function assessed. Preclinical studies and systematic reviews were considered ineligible. <b>Results:</b> The search identified 183 references and 38 were considered eligible. DHEA improved aspects such as sexual interest, lubrication, pain, arousal, orgasm and sexual frequency. Its effect was better in populations with sexual dysfunction, especially in perimenopausal and postmenopausal women. <b>Conclusion:</b> Considering the studies currently published, DHEA is effective in improving several aspects of sexual function, but this effect did not reach all the populations studied.
DOI: 10.6084/m9.figshare.4583584
title: A. mephisto.xlsx

description: Raw morphological data of <i>Aspidoras mephisto</i>, new species
DOI: 10.6084/m9.figshare.4581184
title: Do the top radiology journals utilize reporting guidelines and clinical trial registration? August 2016-January 2017

description: This is the raw data used for the creation of this paper.
DOI: 10.6084/m9.figshare.4583584.v1
title: A. mephisto.xlsx

description: Raw morphological data of <i>Aspidoras mephisto</i>, new species
DOI: 10.6084/m9.figshare.4581184.v1
title: Do the top radiology journals utilize reporting guidelines and clinical trial registration? August 2016-January 2017

description: This is the raw data used for the creation of this paper.
DOI: 10.6084/m9.figshare.4581061
title: FigShare.csv

description: Memory performance (Hit rate and false alarm rate) as well as questionnaire data for each participant included in the analyses. Includes which group participants belong to, age, sex, and education.
DOI: 10.6084/m9.figshare.4581052
title: Supplementary table 1 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Details of the 15 microsatellite loci used in this study together with their polymorphism characteristics in 180 great scallops sampled from nine different populations.
DOI: 10.6084/m9.figshare.4581061.v1
title: FigShare.csv

description: Memory performance (Hit rate and false alarm rate) as well as questionnaire data for each participant included in the analyses. Includes which group participants belong to, age, sex, and education.
DOI: 10.6084/m9.figshare.4581043
title: Supplementary table 4 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Summary of the results of the General Linear Mixed Models (GLMMs) with response variables shell shape PC1 and CI, population included as a random effect, and PC1 and PC2 from the PCA of the SNP dataset fitted as a predictor variables.
DOI: 10.6084/m9.figshare.4581052.v1
title: Supplementary table 1 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Details of the 15 microsatellite loci used in this study together with their polymorphism characteristics in 180 great scallops sampled from nine different populations.
DOI: 10.6084/m9.figshare.4581043.v1
title: Supplementary table 4 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Summary of the results of the General Linear Mixed Models (GLMMs) with response variables shell shape PC1 and CI, population included as a random effect, and PC1 and PC2 from the PCA of the SNP dataset fitted as a predictor variables.
DOI: 10.6084/m9.figshare.4581031
title: Supplementary table 5 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Summary of the results obtained from different de novo assemblies of the ddRAD data generated using different values for three main parameters -m, -M and -n within the denovo_map.pl Script in Stacks. -m and -M define the minimum number of raw reads and the maximum number of mismatches between loci when creating a stack within the same individual respectively. -n corresponds to the number of mismatches allowed between loci when processing multiple individuals. For each tested combination of parameters, we report the total number of tags, the number of tags present in all of the individuals, observed heterozygosity, average depth of coverage, and the number of SNPs obtained after filtering.
DOI: 10.6084/m9.figshare.4581040
title: Supplementary information 1 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Design of the P7 adapters used during the preparation of the ddRAD library.
DOI: 10.6084/m9.figshare.4581031.v1
title: Supplementary table 5 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Summary of the results obtained from different de novo assemblies of the ddRAD data generated using different values for three main parameters -m, -M and -n within the denovo_map.pl Script in Stacks. -m and -M define the minimum number of raw reads and the maximum number of mismatches between loci when creating a stack within the same individual respectively. -n corresponds to the number of mismatches allowed between loci when processing multiple individuals. For each tested combination of parameters, we report the total number of tags, the number of tags present in all of the individuals, observed heterozygosity, average depth of coverage, and the number of SNPs obtained after filtering.
DOI: 10.6084/m9.figshare.4581040.v1
title: Supplementary information 1 from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: Design of the P7 adapters used during the preparation of the ddRAD library.
DOI: 10.6084/m9.figshare.4581022
title: Table S4. Spectral count data for differentially expressed proteins identified between Adult and Nymph CLas(-) ACP. Average spectral count calculated from three biological replicates analyzed from each sample category. Statistical analysis using Fisher's exact test (p-value&lt;0.05 with Hochberg-Benjamini multiple testing correction) was used to determine significance of spectral count differences between categories

description: The Asian citrus psyllid (<i>Diaphorina citri)</i> is the insect vector responsible for the worldwide spread of ‘<i>Candidatus</i> Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such that <i>D. citri</i> transmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in the <i>D. citri</i> immune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host–microbe relationships. Using protein interaction reporter technology, a <i>D. citri</i> haemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with a <i>D. citri</i> myosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact with <i>D. citri</i> proteins. These co-evolved protein interaction networks at the host–microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.
DOI: 10.6084/m9.figshare.4581025
title: Table S5. Annotation of reported PIR crosslinks. Search engine output: react2 data files for each crosslink are listed in the two rightmost columns. React2 files and the corresponding mzXML files have been uploaded to ProteomeXchange as part of this publication. from Protein interaction networks at the host–microbe interface in <i>Diaphorina citri</i>, the insect vector of the citrus greening pathogen

description: The Asian citrus psyllid (<i>Diaphorina citri)</i> is the insect vector responsible for the worldwide spread of ‘<i>Candidatus</i> Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such that <i>D. citri</i> transmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in the <i>D. citri</i> immune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host–microbe relationships. Using protein interaction reporter technology, a <i>D. citri</i> haemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with a <i>D. citri</i> myosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact with <i>D. citri</i> proteins. These co-evolved protein interaction networks at the host–microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.
DOI: 10.6084/m9.figshare.4581019
title: Table S1. Spectral count data for differentially expressed proteins identified between CLas(+)/(-) adult ACP. Average spectral count calculated from three biological replicates analyzed from each sample category. Statistical analysis using Fisher's exact test (p-value&lt;0.05 with Hochberg-Benjamini multiple testing correction) was used to determine significance of spectral count differences between categories

description: The Asian citrus psyllid (<i>Diaphorina citri)</i> is the insect vector responsible for the worldwide spread of ‘<i>Candidatus</i> Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such that <i>D. citri</i> transmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in the <i>D. citri</i> immune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host–microbe relationships. Using protein interaction reporter technology, a <i>D. citri</i> haemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with a <i>D. citri</i> myosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact with <i>D. citri</i> proteins. These co-evolved protein interaction networks at the host–microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.
DOI: 10.6084/m9.figshare.4581025.v1
title: Table S5. Annotation of reported PIR crosslinks. Search engine output: react2 data files for each crosslink are listed in the two rightmost columns. React2 files and the corresponding mzXML files have been uploaded to ProteomeXchange as part of this publication. from Protein interaction networks at the host–microbe interface in <i>Diaphorina citri</i>, the insect vector of the citrus greening pathogen

description: The Asian citrus psyllid (<i>Diaphorina citri)</i> is the insect vector responsible for the worldwide spread of ‘<i>Candidatus</i> Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such that <i>D. citri</i> transmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in the <i>D. citri</i> immune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host–microbe relationships. Using protein interaction reporter technology, a <i>D. citri</i> haemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with a <i>D. citri</i> myosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact with <i>D. citri</i> proteins. These co-evolved protein interaction networks at the host–microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.
DOI: 10.6084/m9.figshare.4581016
title: Table S3. Spectral count data for differentially expressed proteins identified between Adult and Nymph CLas(+) ACP. Average spectral count calculated from three biological replicates analyzed from each sample category. Statistical analysis using Fisher's exact test (p-value&lt;0.05 with Hochberg-Benjamini multiple testing correction) was used to determine significance of spectral count differences between categories

description: The Asian citrus psyllid (<i>Diaphorina citri)</i> is the insect vector responsible for the worldwide spread of ‘<i>Candidatus</i> Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such that <i>D. citri</i> transmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in the <i>D. citri</i> immune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host–microbe relationships. Using protein interaction reporter technology, a <i>D. citri</i> haemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with a <i>D. citri</i> myosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact with <i>D. citri</i> proteins. These co-evolved protein interaction networks at the host–microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.