DOI: 10.5061/dryad.vhhmgqnth
title: Data for: Phylogeography of an endemic California silkmoth genus suggests the importance of an unheralded central California province in generating regional endemic biodiversity

description: The California Floristic province is a biodiversity hotspot. Understanding
 the phylogeographic patterns that exist in this diverse region is
 essential to understanding its evolution and for guiding conservation
 efforts. Calosaturnia is a charismatic silkmoth genus endemic to large
 portions of the region with three described species, C. mendocino, C.
 walterorum, and C. albofasciata. We sampled all three species from across
 their ranges, sequenced 1463 bp of mitochondrial COI and 1941 bp of
 nuclear DNA from three genes, and reconstructed phylogenetic relationships
 and estimated divergence times within the lineages. All three species show
 pronounced evidence of isolation and, in two cases, secondary
 reconnection. An unexpected monophyletic mtDNA lineage was found in the
 Central Coast region, in a region thought to represent an intergrade
 between C. mendocino and C. walterorum. Our genetic data also
 significantly revise previous hypotheses as to which species occur in
 which regions, suggesting that historical ecological changes around four
 Ma ago isolated some lineages, and a secondary isolation event two Ma ago
 led to isolation of populations both in the Central Coast region and the
 southern Sierra Nevada. Our results add to a currently under appreciated
 pattern suggesting that coastal Central California is not a transition
 zone between Northern and Southern California Floristic Province faunas
 but rather its own unique, periodically isolated, biogeographic region.
 They also suggest cryptic diversity may be present in many other currently
 unrecognized groups. Additional research should focus on this central
 California region because many species may be highly restricted in range
 and in need of conservation attention.
description: Sanger sequencing; see paper for further details
description: Data for: Phylogeography of an endemic California silkmoth genus
 suggests the importance of an unheralded central California province in
 generating regional endemic biodiversity
 Supplemental_table_1.xlsx<br> Table with sample collecting
 information and genbank accession numbers for each marker
 Calosaturnia_concatenated_IQtree.pdf<br> Maximum likelihood
 tree generated with IQ-TREE based on the concatenated mtDNA and nuDNA
 alignments
 Calosaturnia_CAD_EF_WG_alignment.fas<br> Multiple sequence
 alignment file in FASTA format with the nuclear markers CAD, EF and
 WG Calosaturnia_CAD_EF_WG_full_tree.pdf<br> The
 full maximum likelihood tree generated with IQ-TREE based on the nuDNA
 (published version has outgroups cropped)
 Calosaturnia_COI5P3P_alignment.fas<br> Multiple sequence
 alignment file in FASTA format with the mitochondrial marker COI
 Calosaturnia_COI5P3P_full_tree_IQTree.pdf<br> The full
 maximum likelihood tree generated with IQ-TREE based on the mtDNA
 (published version has outgroups cropped)
 Calosaturnia_COI5P3P_full_tree_RevBayes.pdf<br> The full
 Bayesian timetree generated with the RevBayes analysis (published version
 has outgroups cropped)
 Calosaturnia_revbayes_molclock.ipynb<br> Jupyter notebook
 with the script used for the RevBayes molecular clock analysis