cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/yvzrx-9g718
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-2/rawdata/</p>tomo1.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-2/rawdata/tomo1.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo1_full.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-2/3dimage_114910/tomo1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo1_full.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-2/3dimage_114961/tomo1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/28365
title: Chandra X-ray Observatory ObsId 28365

description: A search for X-ray emission from the slowest Pulsar
DOI: 10.22002/d8v6j-jdf44
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-12/rawdata/</p>tomo4.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-12/rawdata/tomo4.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo4_full.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-12/3dimage_114921/tomo4_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo4_full.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-12/3dimage_114965/tomo4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.pk9jer
title: Occurrence Download

description: A dataset containing 66597 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Chiroxiphia caudata)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 66597 records from 52 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046582-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/xnjqr-hwk93
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-46/rawdata/</p>Paeruginosa_dFliG11.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-46/rawdata/Paeruginosa_dFliG11.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG11_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-46/3dimage_104218/Paeruginosa_dFliG11_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG11.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-46/rawdata/Paeruginosa_dFliG11.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mmm54-93z95
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-50/rawdata/</p>Paeruginosa_dFliG15.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-50/rawdata/Paeruginosa_dFliG15.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG15_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-50/3dimage_104221/Paeruginosa_dFliG15_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG15.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-50/rawdata/Paeruginosa_dFliG15.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5hnxs-1rm15
title: cryo-CLEM TDP-43

description: Osmotic shock (ask Qing Yao)Over expression of TDP-43-GFP
description: <strong>Tilt Series Date:</strong> 2019-06-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> U2OS cells</p>
<strong>Strain:</strong> TDP-43 expression</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Qing Yao</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on gold funder EM grids</p>
<strong>Sample Preparation:</strong> Manual Botting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-13/rawdata/</p>tomography2_orig.mrc,  Tilt Series (Pixel Size 2.678 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-13/rawdata/tomography2_orig.mrc"
        > <i class="download icon"></i> Download </a></p>

        sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 6.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-13/3dimage_118735/sirt.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography2_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-13/3dimage_118736/tomography2_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vg30r-0mb27
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-61/rawdata/</p>Paeruginosa_dFliG26.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-61/rawdata/Paeruginosa_dFliG26.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG26_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-61/3dimage_104237/Paeruginosa_dFliG26_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG26.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-61/rawdata/Paeruginosa_dFliG26.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.d4d6t6
title: Occurrence Download

description: A dataset containing 151 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acer negundo",
 "Country is Latvia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 151 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046637-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/zt7pg-fdk75
title: Nitrosocaldus yellowstonensis

description: Raw data files of Nitrosocaldus yellowstonensis
description: <strong>Tilt Series Date:</strong> 2019-07-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Candidatus Nitrosocaldus yellowstonensis</p>
<strong>Strain:</strong> HL72</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jose R De La Torre and students, SF state, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R0.6/1 copper 300 mesh, 10nm gold, 22deg C, 100% humidity, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-11/3dimage_105634/</p>20190715_Ny_stationary_3_full.rec,  Reconstruction (Pixel Size 1.0712 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-11/3dimage_105634/20190715_Ny_stationary_3_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_3.mrc,  Tilt Series (Pixel Size 0.2678 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-11/rawdata/20190715_Ny_stationary_3.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_3.mrc.mdoc,  32.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-11/rawdata/20190715_Ny_stationary_3.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.3hkybs
title: Occurrence Download

description: A dataset containing 9 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pelasgus marathonicus",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Geometry POLYGON ((-6.6 29.26705, -6.6 50.94, 40.81373 50.94, 40.81373 29.26705, -6.6 29.26705))",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Observation, Material sample)"
 ]
}

 The dataset includes 9 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046636-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bxppx-nrv89
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-54/rawdata/</p>Paeruginosa_dFliG19.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-54/rawdata/Paeruginosa_dFliG19.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG19_part61_2.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-54/3dimage_104228/Paeruginosa_dFliG19_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG19.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-54/rawdata/Paeruginosa_dFliG19.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7jqgt-gxt70
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-6/rawdata/</p>tomo13.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-6/rawdata/tomo13.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo13_full.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-6/3dimage_114922/tomo13_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo13_full.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-6/3dimage_114964/tomo13_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z1v0n-c5x98
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-67/rawdata/</p>Paeruginosa_dFliG32.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-67/rawdata/Paeruginosa_dFliG32.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG32_part61_2.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-67/3dimage_104240/Paeruginosa_dFliG32_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG32.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-67/rawdata/Paeruginosa_dFliG32.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>