cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11804529
title: Impact of the MELD score on the survival of hepatocellular carcinoma transplantation patients in Brazil: a systematic review.

description: ABSTRACT This study aimed to analyse the predictive value of Model For End-Stage Liver Disease (MELD) score on medium- and long-term survival in transplanted hepatocellular carcinoma (HCC) patients in Brazil. The study was registered with International Prospective Register of Systematic Reviews (PROSPERO) under N# 152,363. Inclusion criteria were based on Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) recommendations. The search was performed on the indexed databases of Lilacs, SciELO, PubMed, and Cochrane Library, and used as search strategy the following Medical Subject Headings (MeSH) terms: (((“MELD Score”) OR “Model For End-Stage Liver Disease”) AND “Hepatocellular Carcinoma”) AND (“Brazil”). We included full-text articles published from January 2006 to October 2019. The initial search found 162 articles. After reading the available abstracts and full texts, 156 articles were excluded, totaling six articles for qualitative analysis. Although the small number of eligible articles was a limiting factor of the study, our results partially corroborated those found in the United States, United Kingdom, and Ireland. In these countries, unlike Brazil, MELD prognostic model has shown a strong association with post-liver transplant (LT) survival. However, the low predictive capacity of the model in medium- and long-term has been similar to the one of our study. The urgency of the development and validation of a post-transplant survival model for patients with HCC is set, improving the organ allocation system in Brazil.
DOI: 10.6084/m9.figshare.11804523
title: Effect of probiotics and Yucca schidigera extract supplementation on broiler meat quality

description: ABSTRACT. The current study investigated the effect of dietary supplementation with probiotics and Yucca schidigera extract on physicochemical parameters, proximate composition, mineral content and fatty acid profile of broiler breast and thigh muscles. In total, 240 one-day old broilers were randomly allocated into two dietary treatments groups: 1) Control (basal diet), 2) experimental (basal diet with two probiotics Pediococcus acidilactici and Saccharomyces cerevisiae and Yucca schidigera extract). The results showed that the pH value was higher in the experimental group than in the control group (p &lt; 0.05). However, drip, cook and thaw losses were not influenced by dietary treatment (p &gt; 0.05). A significant increase in protein, Fe, Zn, Na, P and a significant decrease in lipid, Cu and Cr contents was exhibited in experimental group relative to control group (p &lt; 0.05). The proportion of stearic acid and saturated fatty acids was significantly (p &lt; 0.05) reduced, whereas linoleic acid and polyunsaturated fatty acids contents were significantly (p &lt; 0.05) increased in breast and thigh muscles of fed the experimental diet. We concluded that additive supplementation of the diet with probiotics and Yucca schidigera extract could improve meat quality.
DOI: 10.6084/m9.figshare.11804526.v1
title: Assessment of Culex pipiens bioforms in the world’s southernmost distribution limit

description: The mosquito Culex pipiens s.s. L. occurs as two bioforms that differ in physiology and behaviour affecting virus transmission cycles. To assess the occurrence of Cx. pipiens bioforms in the southernmost limit of its distribution, specimens were collected aboveground in southern Buenos Aires Province and east Patagonia, Argentina. Ten larvae and 25 adults were individually processed and identified by polymerase chain reaction (PCR) amplification of Ace-2 and CQ11 loci. Culex quinquefasciatus Say (one larva, two adults), Cx. pipiens f. molestus (one larva, one adult) and one adult of hybrid origin were identified in Buenos Aires Province; only Cx. pipiens f. molestus was recorded in Patagonia (eight larvae, 21 adults). The potential absence of bioform pipiens and its implications in arbovirus enzootic cycles is discussed.
DOI: 10.6084/m9.figshare.11804523.v1
title: Effect of probiotics and Yucca schidigera extract supplementation on broiler meat quality

description: ABSTRACT. The current study investigated the effect of dietary supplementation with probiotics and Yucca schidigera extract on physicochemical parameters, proximate composition, mineral content and fatty acid profile of broiler breast and thigh muscles. In total, 240 one-day old broilers were randomly allocated into two dietary treatments groups: 1) Control (basal diet), 2) experimental (basal diet with two probiotics Pediococcus acidilactici and Saccharomyces cerevisiae and Yucca schidigera extract). The results showed that the pH value was higher in the experimental group than in the control group (p &lt; 0.05). However, drip, cook and thaw losses were not influenced by dietary treatment (p &gt; 0.05). A significant increase in protein, Fe, Zn, Na, P and a significant decrease in lipid, Cu and Cr contents was exhibited in experimental group relative to control group (p &lt; 0.05). The proportion of stearic acid and saturated fatty acids was significantly (p &lt; 0.05) reduced, whereas linoleic acid and polyunsaturated fatty acids contents were significantly (p &lt; 0.05) increased in breast and thigh muscles of fed the experimental diet. We concluded that additive supplementation of the diet with probiotics and Yucca schidigera extract could improve meat quality.
DOI: 10.6084/m9.figshare.11804529.v1
title: Impact of the MELD score on the survival of hepatocellular carcinoma transplantation patients in Brazil: a systematic review.

description: ABSTRACT This study aimed to analyse the predictive value of Model For End-Stage Liver Disease (MELD) score on medium- and long-term survival in transplanted hepatocellular carcinoma (HCC) patients in Brazil. The study was registered with International Prospective Register of Systematic Reviews (PROSPERO) under N# 152,363. Inclusion criteria were based on Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) recommendations. The search was performed on the indexed databases of Lilacs, SciELO, PubMed, and Cochrane Library, and used as search strategy the following Medical Subject Headings (MeSH) terms: (((“MELD Score”) OR “Model For End-Stage Liver Disease”) AND “Hepatocellular Carcinoma”) AND (“Brazil”). We included full-text articles published from January 2006 to October 2019. The initial search found 162 articles. After reading the available abstracts and full texts, 156 articles were excluded, totaling six articles for qualitative analysis. Although the small number of eligible articles was a limiting factor of the study, our results partially corroborated those found in the United States, United Kingdom, and Ireland. In these countries, unlike Brazil, MELD prognostic model has shown a strong association with post-liver transplant (LT) survival. However, the low predictive capacity of the model in medium- and long-term has been similar to the one of our study. The urgency of the development and validation of a post-transplant survival model for patients with HCC is set, improving the organ allocation system in Brazil.
DOI: 10.6084/m9.figshare.11804499
title: Origins of life in the cosmic context: the first MOOC in astronomy developed in Brazil

description: Abstract “Origins of life in the cosmic context” was the first one Astronomy MOOC in Portuguese-language. The launch took place in June 2016, initially counting more than 4,000 students, which gradually (and cumulatively) until the last session analyzed (august, 2019). This paper shows the MOOC methodology that already attended more than 150 thousands students and a discussion about the maintenance in a mentored and independent way. As a result, we found that the course completion rate was around 9%, which corroborates the literature, in addition to the predominance of higher education students and males. The students satisfaction level was high: on a scale of 0 to 5, satisfaction was assessed at 4.8. Moreover, the course has already transcended the virtual world barrier and have been used as a tool to aid astronomy learning in elementary and middle schools.
DOI: 10.6084/m9.figshare.11804499.v1
title: Origins of life in the cosmic context: the first MOOC in astronomy developed in Brazil

description: Abstract “Origins of life in the cosmic context” was the first one Astronomy MOOC in Portuguese-language. The launch took place in June 2016, initially counting more than 4,000 students, which gradually (and cumulatively) until the last session analyzed (august, 2019). This paper shows the MOOC methodology that already attended more than 150 thousands students and a discussion about the maintenance in a mentored and independent way. As a result, we found that the course completion rate was around 9%, which corroborates the literature, in addition to the predominance of higher education students and males. The students satisfaction level was high: on a scale of 0 to 5, satisfaction was assessed at 4.8. Moreover, the course has already transcended the virtual world barrier and have been used as a tool to aid astronomy learning in elementary and middle schools.
DOI: 10.6084/m9.figshare.11804490
title: Prediction of soil organic matter and clay contents by near-infrared spectroscopy - NIRS

description: ABSTRACT: Among the soil constituents, special attention is given to soil organic matter (SOM) and clay contents, since, among other aspects, they are key factors to nutrient retention and soil aggregates formation, which directly affect the crop production potential. The methods commonly used for the quantification of these constituents have some disadvantages, such as the use of chemical reactants and waste generation. An alternative to these methods is the near-infrared spectroscopy (NIRS) technique. The aim of this research is to evaluate models for SOM and clay quantification in soil samples using spectral data by NIRS. A set (n = 400) of soil samples previously analyzed by traditional methods were used to generate a NIRS calibration curve. The clay content was determined by the hydrometer method while SOM content was determined by sulfochromic solution. For calibration, we used the original spectra (absorbance) and spectral pretreatment (Savitzky-Golay smoothing derivative) in the following models: multiple linear regression (MLR), partial last squares regression (PLSR), support vector machine (SVM) and Gaussian process regression (GPR). The curve validation was performed with the SVM model (best performance in the calibration based on R² and RMSE) in two ways: with 40 random samples from the calibration set and another set with 200 new unknown samples. The soil clay content affects the predictive ability of the calibration curve to estimate SOM content by NIRS. Validation curves showed poorer performance (lower R² and higher RMSE) when generated from unknown samples, where the model tends to overestimate the lower levels and to underestimate the higher levels of clay and SOM. Despite the potential of NIRS technique to predict these attributes, further calibration studies are still needed to use this technique in soil analysis laboratories.
DOI: 10.6084/m9.figshare.11804490.v1
title: Prediction of soil organic matter and clay contents by near-infrared spectroscopy - NIRS

description: ABSTRACT: Among the soil constituents, special attention is given to soil organic matter (SOM) and clay contents, since, among other aspects, they are key factors to nutrient retention and soil aggregates formation, which directly affect the crop production potential. The methods commonly used for the quantification of these constituents have some disadvantages, such as the use of chemical reactants and waste generation. An alternative to these methods is the near-infrared spectroscopy (NIRS) technique. The aim of this research is to evaluate models for SOM and clay quantification in soil samples using spectral data by NIRS. A set (n = 400) of soil samples previously analyzed by traditional methods were used to generate a NIRS calibration curve. The clay content was determined by the hydrometer method while SOM content was determined by sulfochromic solution. For calibration, we used the original spectra (absorbance) and spectral pretreatment (Savitzky-Golay smoothing derivative) in the following models: multiple linear regression (MLR), partial last squares regression (PLSR), support vector machine (SVM) and Gaussian process regression (GPR). The curve validation was performed with the SVM model (best performance in the calibration based on R² and RMSE) in two ways: with 40 random samples from the calibration set and another set with 200 new unknown samples. The soil clay content affects the predictive ability of the calibration curve to estimate SOM content by NIRS. Validation curves showed poorer performance (lower R² and higher RMSE) when generated from unknown samples, where the model tends to overestimate the lower levels and to underestimate the higher levels of clay and SOM. Despite the potential of NIRS technique to predict these attributes, further calibration studies are still needed to use this technique in soil analysis laboratories.
DOI: 10.6084/m9.figshare.11804484
title: Association of Menopausal Status, Expression of Progesterone Receptor and Ki67 to the Clinical Response to Neoadjuvant Chemotherapy in Luminal Breast Cancer

description: Abstract Objective To identify the biomarkers of response to neoadjuvant chemotherapy in early luminal breast cancer. Methods A cross-sectional study that included all patients with early or locallyadvanced luminal breast cancer submitted to neoadjuvant chemotherapy between 2013 and 2014. Demographic, clinic and pathologic data were retrieved from patient records. The expressions of the estrogen receptor (ER), the progesterone receptor (PR), and Ki67 were analyzed by immunohistochemistry (IHC). The status of the human epidermal growth factor receptor 2 (HER2) was evaluated by IHC and fluorescent in situ hybridization (FISH). Independent predictors of clinic and pathologic response were evaluated by stepwise logistic regression models and receiver operating characteristic (ROC) curve analysis. Results Out of 298 patients identified, 115 were included in the analysis. Clinical complete response (cCR) was observed in 43.4% of the patients (49/113), and pathologic complete response (pCR) was observed in 7.1% (8/115) of the patients. The independent predictors of cCR were premenopausal status (p &lt; 0.001), low PR expression (≤ 50% versus &gt; 50%; p = 0.048), and Ki67 expression ≥ 14% (versus &lt; 14%; p = 0.01). Patients with cCR were more commonly submitted to breast conserving surgery (34.7% versus 7.8%; p &lt; 0.001). Increasing cut-off points for Ki67 expression were associated with an increase in specificity and a decrease in sensitivity to identify patients with cCR. Conclusion Premenopausal status, lower PR expression and higher Ki67 expression were associated with a higher rate of cCR to neoadjuvant chemotherapy in luminal breast cancer.
DOI: 10.6084/m9.figshare.11804481
title: Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia

description: Background The Eastern Russell’s viper, Daboia siamensis, is a WHO Category 1 medically important venomous snake. It has a wide but disjunct distribution in Southeast Asia. The specific antivenom, D. siamensis Monovalent Antivenom (DsMAV-Thailand) is produced in Thailand but not available in Indonesia, where a heterologous trivalent antivenom, Serum Anti Bisa Ular (SABU), is used instead. This study aimed to investigate the geographical venom variation of D. siamensis from Thailand (Ds-Thailand) and Indonesia (Ds-Indonesia), and the immunorecognition of the venom proteins by antivenoms. Methods: The venom proteins were decomplexed with reverse-phase high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by in-solution tryptic digestion, nano-liquid chromatography-tandem mass spectrometry and protein identification. The efficacies of DsMAV-Thailand and SABU in binding the various venom fractions were assessed using an enzyme-linked immunosorbent assay optimized for immunorecognition profiling. Results: The two most abundant protein families in Ds-Thailand venom are phospholipase A2 (PLA2) and Kunitz-type serine protease inhibitor (KSPI). Those abundant in Ds-Indonesia venom are PLA2 and serine protease. KSPI and vascular endothelial growth factor were detected in Ds-Thailand venom, whereas L-amino acid oxidase and disintegrin were present in Ds-Indonesia venom. Common proteins shared between the two included snaclecs, serine proteases, metalloproteinases, phosphodiesterases, 5’nucleotidases and nerve growth factors at varying abundances. DsMAV-Thailand exhibited strong immunorecognition of the major protein fractions in both venoms, but low immunoreactivity toward the low molecular weight proteins e.g. KSPI and disintegrins. On the other hand, SABU was virtually ineffective in binding all fractionated venom proteins. Conclusion: D. siamensis venoms from Thailand and Indonesia varied geographically in the protein subtypes and abundances. The venoms, nevertheless, shared conserved antigenicity that allowed effective immunorecognition by DsMAV-Thailand but not by SABU, consistent with the neutralization efficacy of the antivenoms. A specific, appropriate antivenom is needed in Indonesia to treat Russell’s viper envenomation.
DOI: 10.6084/m9.figshare.11804484.v1
title: Association of Menopausal Status, Expression of Progesterone Receptor and Ki67 to the Clinical Response to Neoadjuvant Chemotherapy in Luminal Breast Cancer

description: Abstract Objective To identify the biomarkers of response to neoadjuvant chemotherapy in early luminal breast cancer. Methods A cross-sectional study that included all patients with early or locallyadvanced luminal breast cancer submitted to neoadjuvant chemotherapy between 2013 and 2014. Demographic, clinic and pathologic data were retrieved from patient records. The expressions of the estrogen receptor (ER), the progesterone receptor (PR), and Ki67 were analyzed by immunohistochemistry (IHC). The status of the human epidermal growth factor receptor 2 (HER2) was evaluated by IHC and fluorescent in situ hybridization (FISH). Independent predictors of clinic and pathologic response were evaluated by stepwise logistic regression models and receiver operating characteristic (ROC) curve analysis. Results Out of 298 patients identified, 115 were included in the analysis. Clinical complete response (cCR) was observed in 43.4% of the patients (49/113), and pathologic complete response (pCR) was observed in 7.1% (8/115) of the patients. The independent predictors of cCR were premenopausal status (p &lt; 0.001), low PR expression (≤ 50% versus &gt; 50%; p = 0.048), and Ki67 expression ≥ 14% (versus &lt; 14%; p = 0.01). Patients with cCR were more commonly submitted to breast conserving surgery (34.7% versus 7.8%; p &lt; 0.001). Increasing cut-off points for Ki67 expression were associated with an increase in specificity and a decrease in sensitivity to identify patients with cCR. Conclusion Premenopausal status, lower PR expression and higher Ki67 expression were associated with a higher rate of cCR to neoadjuvant chemotherapy in luminal breast cancer.
DOI: 10.6084/m9.figshare.11804481.v1
title: Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia

description: Background The Eastern Russell’s viper, Daboia siamensis, is a WHO Category 1 medically important venomous snake. It has a wide but disjunct distribution in Southeast Asia. The specific antivenom, D. siamensis Monovalent Antivenom (DsMAV-Thailand) is produced in Thailand but not available in Indonesia, where a heterologous trivalent antivenom, Serum Anti Bisa Ular (SABU), is used instead. This study aimed to investigate the geographical venom variation of D. siamensis from Thailand (Ds-Thailand) and Indonesia (Ds-Indonesia), and the immunorecognition of the venom proteins by antivenoms. Methods: The venom proteins were decomplexed with reverse-phase high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by in-solution tryptic digestion, nano-liquid chromatography-tandem mass spectrometry and protein identification. The efficacies of DsMAV-Thailand and SABU in binding the various venom fractions were assessed using an enzyme-linked immunosorbent assay optimized for immunorecognition profiling. Results: The two most abundant protein families in Ds-Thailand venom are phospholipase A2 (PLA2) and Kunitz-type serine protease inhibitor (KSPI). Those abundant in Ds-Indonesia venom are PLA2 and serine protease. KSPI and vascular endothelial growth factor were detected in Ds-Thailand venom, whereas L-amino acid oxidase and disintegrin were present in Ds-Indonesia venom. Common proteins shared between the two included snaclecs, serine proteases, metalloproteinases, phosphodiesterases, 5’nucleotidases and nerve growth factors at varying abundances. DsMAV-Thailand exhibited strong immunorecognition of the major protein fractions in both venoms, but low immunoreactivity toward the low molecular weight proteins e.g. KSPI and disintegrins. On the other hand, SABU was virtually ineffective in binding all fractionated venom proteins. Conclusion: D. siamensis venoms from Thailand and Indonesia varied geographically in the protein subtypes and abundances. The venoms, nevertheless, shared conserved antigenicity that allowed effective immunorecognition by DsMAV-Thailand but not by SABU, consistent with the neutralization efficacy of the antivenoms. A specific, appropriate antivenom is needed in Indonesia to treat Russell’s viper envenomation.
DOI: 10.6084/m9.figshare.11804460
title: Misorientation Changes and Residual Stresses Redistribution after Welding. A Physical Simulation

description: Welding is widely used in the manufacture of steel products. Metallurgical processes during welding produce residual stresses in different zones of the welded parts. Welding residual stresses, particularly tensile stresses, can significantly impact the reliability and the integrity of the welded components. Our recent works report the effect of residual stress redistribution in a relatively short, up to two weeks, period after the welding procedure. The hypothesis that the observed stress relaxation phenomenon is associated to the microstructure evolution that occur in the material after the end of the welding procedure was proposed. To prove this hypothesis a physical simulation of the influence of the welding thermal cycles on the base metal with GLEEBLE equipment was performed. Using SEM EBSD, technique the variation of grain boundary misorientation accompanied by the residual stresses redistribution in the first days after welding thermal cycles simulation were observed.