DOI: 10.17045/sthlmuni.13211204
title: Rodrigues et al. Fluctuating heat stress data

description: <b>Summary:</b><b><br></b> This data was used to assess how fluctuating sublethal heat stress during development impacted subsequent male reproductive performance and success. Using a subset of isogenic lines of the Drosophila Genetic Reference Panel (DGRP) reared at standardized densities in four thermal conditions (Constant 25°C, Constant 29°C, Fluctuating 25°C, Fluctuating 29°C), we quantified phenotypic (and genetic) variation in mating propensity, fertility, productivity and sex-ratio. <br> <b>Files available:</b> We provide 2 txt files: FLUCT_mating_fertility; FLUCT_productivity_sexratio. <br> <b>FLUCT_mating_fertility: </b> This file contains the raw data of male mating propensity and fertility under four thermal conditions. Table headers are explained below: <i>LineStatus</i> - classification based on the reproductive performance across three temperatures tested in Zwoinska et al. (2020)*. (High – lines whose fertility did not decline as temperature increased; Low – lines whose fertility declined substantially as temperature increased) <i>DGRP</i>- line identity <i>Wolbachia</i>- line infection status (y – infected; n- uninfected) <i>Haplotype</i>- haplotypes identified in Bevers et al. (2019)* <i>Inv1/2/7/8/9</i> - the inversion status of each line for each of the main inversions characterized in the DGRP (ST = standard, INV = inversion. ST/INV = unfixed status in the line) <i>Temperature</i> - mean developmental temperature experienced (25°C or 29°C) <i>ThermalRegime</i> - thermal variation experienced (Const - constant or Fluct - fluctuating regime) <i>Vial</i> – vial where each pair was kept <i>EggCollDay</i> – day the eggs were collected and placed in one of the thermal conditions. <i>PairingDay</i> – day males and females were paired <i>Mating</i> – mating propensity (1, if mating observed; 0, if no mating observed) <i>Larvae</i> – fertility (1, if larvae present; 0, if no larvae present)<br> <b>FLUCT_productivity_sexratio: </b> This file contains the raw data of male productivity and sex ratio under four thermal conditions. Table headers are explained below: <i>LineStatus</i> - classification based on the reproductive performance across three temperatures tested in Zwoinska et al. 2020. (High - whose fertility did not decline as temperature increased; Low - whose fertility declined substantially as temperature increased) <i>DGRP</i>- line identity <i>Wolbachia</i>- line infection status (y – infected; n- uninfected) <i>Haplotype</i>- haplotypes identified in Bevers et al. (2019)* <i>Inv1/2/7/8/9</i> - the inversion status of each line for each of the main inversions characterized in the DGRP (ST = standard, INV = inversion. ST/INV = unfixed status in the line) <i>Temperature</i> - mean developmental temperature experienced (25°C or 29°C) <i>ThermalRegime</i> - thermal variation experienced (Const - constant or Fluct - fluctuating regime) <i>Vial</i> – vial where each pair was kept <i>EggCollDay</i> – day the eggs were collected and placed in one of the thermal conditions. <i>PairingDay</i> – day males and females were paired <i>Productivity</i> – number of adult offspring produced. <i>Daughters</i> – number of female offspring produced. <i>Sons</i> – number of male offspring produced. <i>IGV</i> – absolute deviation from the median as a measure of intra-genotypic variability. *Bevers, R. P. J., et al (2019). Mitochondrial haplotypes affect metabolic phenotypes in the Drosophila Genetic Reference Panel. Nature Metabolism, 1, 1226–1242. https://doi.org/10.1038/s42255-019-0147-3 Zwoinska, M. K., Rodrigues, L. R., Slate, J., & Snook, R. R. (2020). Phenotypic responses to and genetic architecture of sterility in response to sub-lethal temperature during development. Frontiers in Genetics, 11, 573. https://doi.org/10.3389/fgene.2020.00573