DOI: 10.5061/dryad.q6q1726
title: Data from: Spruce and beech as local determinants of forest fungal community structure in litter, humus and mineral soil

description: Beech forests reaches its native distribution limit in SE Norway, but is
 expected to expand substantially northwards due to climate warming. This
 may potentially result in a fundamental transformation of contemporary
 Northern European forests, with tentative effects on the associated
 belowground fungi. Fungal communities mediate vital ecosystem processes
 such as ecosystem productivity and carbon sequestration in boreal forests.
 To investigate how soil fungi is affected by the vegetation transition
 from spruce to beech forest, we sampled litter, humus and mineral soil in
 a forest landscape dominated by beech, spruce or a mixture of these. The
 fungal communities in the soil samples were analyzed by DNA metabarcoding
 of the rDNA ITS2 region. Although soil layers were the most important
 structuring gradient, we found clear differences in fungal species
 composition between spruce and beech plots. The differences in fungal
 community composition were most evident in the litter and least in the
 mineral soil. Decomposers, most notably Mycena, dominated the litter layer
 while various mycorrhizal fungi dominated the humus and mineral layers.
 Some ectomycorrhizal taxa, such as Cenoccocum and Russula, were more
 abundant in spruce forests. Differences in fungal community composition
 between forest types can potentially have large impacts on carbon
 sequestration rates.
description: Representative sequences
 for all OTUsUnclustered
 representative sequences for all OTUsFF_ITS_NC_uclustref_repset.fnaFF7_ITS2mapBarcode and primer mapping fileFF8_ITS2mapBarcode and primer mapping fileFinal OTU/Sample data matrixOTU-table with taxonomic annotation for OTUs and information on sample treatmentsFinal OTU-Sample data matrix.xlsxRaw sequences (forward reads) first partFirst part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R1_001.fastq.gz.001Raw sequences (forward reads) second partSecond part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R1_001.fastq.gz.002Raw sequences (forward reads) third partThird part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R1_001.fastq.gz.003Raw sequences (reverse reads) first partFirst part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.001Raw sequences (reverse reads) second partSecond part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.002Raw sequences (reverse reads) third partThird part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.003Raw sequences (reverse reads) fourth partFourth part of the bzip-compressed fastq-file containing raw sequences (reverse reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).FFL8_S1_L001_R2_001.fastq.gz.004