DOI: 10.5061/dryad.580bh
title: Data from: Transgenerational plasticity and environmental stress: do paternal effects act as a conduit or a buffer?

description: For most organisms, early life-history stages are the most sensitive to
 environmental stress and so transgenerational phenotypic plasticity,
 whereby the parental environment and offspring environment interact to
 alter the phenotype of the offspring, is viewed as key to promoting
 persistence in the face of environmental change. While there has been
 long-standing interest in the role of transgenerational plasticity via the
 maternal line (traditionally the field of maternal effects), increasingly
 it appears that paternal effects can also play a role. Despite the
 emerging role of paternal effects in studies of global change, key
 knowledge gaps remain: first, whether paternal effects act to increase or
 decrease offspring performance remains largely unexplored; second, the
 relative roles of maternal and paternal effects are rarely disentangled;
 and third, the role of environmental variation, a key determinant of the
 benefits of transgenerational plasticity, has not been explored with
 regard to paternal effects. Here, we explore all three issues using the
 marine tubeworm Galeolaria caespitosa, an important habitat-forming
 species in southern Australia. We found that both paternal and maternal
 experiences affected key stages of offspring performance (fertilization
 and larval development) and, surprisingly, paternal effects were often
 stronger than maternal effects. Furthermore, we found that paternal
 effects often reduced offspring performance, especially when environments
 varied compared with when environments were stable. Our results suggest
 that, while transgenerational plasticity may play an important role in
 modifying the impacts of global change, these effects are not uniformly
 positive. Importantly, paternal effects can be as strong, or stronger,
 than maternal effects and environmental variability strongly alters the
 impacts of paternal effects.
description: Guillaume et al
 2015_Transgenerational plasticity_Fertilisation success
 dataGaleolaria caespitosa
 were collected from Brighton marina, Melbourne, Australia in 2013. Adults
 were brought back to Monash University for two weeks acclimation prior to
 fertilisation assays. The workbook contains three sheets:
 'Fertilisation success', 'Egg measurements';
 'Sperm measurements'. The descriptions of column headings are
 available in the ReadMe file.Guillaume et al
 2015_Transgenerational plasticity_Offspring survival
 dataGaleolaria caespitosa
 were collected from Brighton marina, Melbourne, Australia in 2013 and
 2014. Adults were brought back to Monash University for two weeks
 acclimation prior to larval survival experiments. The column headings are
 as follows: 'Run' = assay number; 'Date_start' =
 Starting date for adult acclimation; 'Days_acclimate' = Number
 of days adults were left to acclimate in their treatment conditions;
 'Paternal_envt' = male treatment acclimation temperature (cold
 ~16.5 deg C; hot ~22.0 deg C); 'Maternal_envt' = female
 treatment acclimation temperature (cold ~16.5 deg C; hot ~22.0 deg C);
 'Offspring_envt' = Fertilisation and incubation temperature of
 offspring; 'Replicate' = replicated male*female*offspring
 treatment temperatures A and B; 'Number_eggs in' = Number of
 successfully fertilised eggs selected for treatment; 'Number_larvae
 developed' = Number of larvae that developed from the selected eggs;
 'Percent_survival' = Percent of larvae that developed from the
 selected eggs.Guillaume et al
 2015_Transgenerational plasticity_Offspring survival switched male
 dataGaleolaria caespitosa
 were collected from Brighton marina, Melbourne, Australia in 2014. Adults
 were brought back to Monash University for a total of four weeks
 acclimation prior to larval survival experiments. At two weeks into the
 acclimation period, half of the males changed acclimation treatment
 temperatures. The column headings are as follows: 'Run' = assay
 number; 'Date_start' = Starting date for adult acclimation;
 'Paternal_envt1' = the male temperature treatment experienced
 for the first two weeks of acclimation (cold ~16.5 deg C; hot ~22.0 deg
 C); 'Paternal_envt2' = the male temperature treatment
 experienced for the final two weeks of acclimation (cold ~16.5 deg C; hot
 ~22.0 deg C); 'Maternal_envt' = female treatment acclimation
 temperature (cold ~16.5 deg C; hot ~22.0 deg C);
 'Offspring_envt' = Fertilisation and incubation temperature of
 offspring; 'Replicate' = replicated male*female*offspring
 treatment temperatures A and B; 'Number_eggs in' = Number of
 successfully fertilised eggs selected for treatment; 'Number_larvae
 developed' = Number of larvae that developed from the selected eggs;
 'Percent_survival' = Percent of larvae that developed from the
 selected eggs.Guillaume at al
 2015_Transgenerational plasticity_Brighton Marina temperature
 Mar-SepTemperature data
 collected at Middle Brighton Pier, Melbourne, Australia between March and
 September in 2013. A data logger (Onset HOBO Pendant data logger) was
 placed adjacent to Galeolaria caespitosa clusters used in experiments.
 Column headings: 'Date (MM/DD/YY) = date that data was collected in
 US format; ' Time' = hour, minute and second that data was
 collected in 12h format; 'Temp, °C' = Temperature recorded in
 degrees Celsius.