DOI: 10.5061/dryad.37pvmcvtk
title: Molecular data of cryptic species in the Sphagnum magellanicum complex (Bryophyta) in North America

description: Sphagnum magellanicum (Sphagnaceae, Bryophyta) has been considered to be a
 single semi-cosmopolitan species, but recent molecular analyses have shown
 that it comprises a complex of at least seven reciprocally monophyletic
 groups, that are, however, difficult or impossible to distinguish
 morphologically. Newly developed barcode markers and RADseq
 analyses were used to identify species among 808 samples from 119 sites.
 Molecular approaches are used here to assess the geographic ranges of four
 North American species, the frequency at which they occur sympatrically,
 and ecological differentiation among them. Microhabitats were classified
 with regard to hydrology and shade. Hierarchical Modelling of Species
 Communities was used to assess climate variation among the species.
 Climate niches were projected back to 22,000 years bp to assess the
 likelihood that the North American species had sympatric ranges during the
 late Pleistocene. The species exhibit parallel morphological
 variation, making it extremely difficult to distinguish phenotypically.
 Two to three species frequently co-occur within peatlands. They have
 broadly overlapping micro-habitat and climate niches. Barcode- versus
 RADseq-based identifications were in conflict for 6% of the samples, and
 always involved S. diabolicum vs. S. magniae. These species
 co-occur within peatlands at scales that could permit interbreeding, yet
 they remain largely distinct genetically and phylogenetically. The four
 cryptic species exhibit individualistic geographic and ecological
 patterns. Conflicting identifications from barcode vs. RADseq analyses for
 S. diabolicum versus S. magniae could reflect incomplete speciation or
 hybridization. They comprise a valuable study system for additional work
 on climate adaptation.
description: # Ecological differentiation and sympatry of cryptic species in the
 *Sphagnum magellanicum* complex (Bryophyta)
 [https://doi.org/10.5061/dryad.37pvmcvtk](https://doi.org/10.5061/dryad.37pvmcvtk) Principal Investigator/Corresponding author: Marta Nieto-Lugilde, Duke University, [marta.nietolugilde@duke.edu](mailto:marta.nietolugilde@duke.edu) Data prepared by: Marta Nieto-Lugilde, Duke University, [marta.nietolugilde@duke.edu](mailto:marta.nietolugilde@duke.edu) Co-investigators: Diego Nieto-Lugilde, Universidad de Cordoba Bryan Piatkowski, Oak Ridge National Laboratory Aaron M. Duffy, Duke University Sean C. Robinson, SUNY Oneonta Blanka Aguero, Duke University Scott Schuette, Pennsylvania Natural Heritage Program Richard Wilkens, Salisbury University Joseph Yavitt, Cornell University A. Jonathan Shaw, Duke University Date collected: 2019-2024 These datasets DNA sequences for *Sphagnum magellanicum* group plants collected from the following countries: Canada (Alberta, British Columbia, Labrador, New Brunswick, Newfoundland, Nova Scotia, Quebec), USA (Alabama, Alaska, Arkansas, Connecticut, Delaware, Florida, Georgia, Kentucky, Maine, Maryland, Michigan, Minnesota, Mississippi, New Hampshire, New Jersey, New York, North Carolina, Pennsylvania, South Carolina, Tennessee, Vermont, Virginia, Washington, West Virginia, Wisconsin). Collection locations for specific samples included in this dataset can be found in Table S1 of the associated manuscript. ## Data and file overview: bcdp-80p-633_samples.phy: Phylip format alignment of 2140 RADseq loci generated by ipyrad for *Sphagnum* samples. demultiplexed_sample_data-insilico.tar.gz: Zipped folder of zipped fastq format "RADseq-like" reads from 66 in silico digested genome resequencing samples. 10 copies of each read (enough to pass downstream depth filters) with quality values all set to "E" (high enough to pass downstream quality filters). File names match the sample name. 2024-06-03 demultiplexed_sample_data-radseq1.tar.gz: Zipped folder of demultiplexed zipped fastq format Illumina reads from 100 *Sphagnum* RADseq samples. File names match the sample name. 2024-06-03 demultiplexed_sample_data-radseq2.tar.gz: Zipped folder of demultiplexed zipped fastq format Illumina reads from 100 *Sphagnum* RADseq samples. File names match the sample name. 2024-06-03 demultiplexed_sample_data-radseq3.tar.gz: Zipped folder of demultiplexed zipped fastq format Illumina reads from 100 *Sphagnum* RADseq samples. File names match the sample name. 2024-06-03 demultiplexed_sample_data-radseq4.tar.gz: Zipped folder of demultiplexed zipped fastq format Illumina reads from 100 *Sphagnum* RADseq samples. File names match the sample name. 2024-06-03 demultiplexed_sample_data-radseq5.tar.gz: Zipped folder of demultiplexed zipped fastq format Illumina reads from 100 *Sphagnum* RADseq samples. File names match the sample name. 2024-06-03 demultiplexed_sample_data-radseq6.tar.gz: Zipped folder of demultiplexed zipped fastq format Illumina reads from 67 *Sphagnum* RADseq samples. File names match the sample name. 2024-06-03 md5sum.txt: A text file containing md5 checksum values for each of the data files. 2024-06-03 Additional information on specific samples included in this dataset can be found in Table S1 of the associated manuscript. ## Sharing and access information: This work is licensed under a CC0 1.0 Universal (CC0 1.0) Public Domain Dedication license.