DOI: 10.5061/dryad.vp530
title: Data from: Temporal dynamics of plant-soil feedback and root-associated fungal communities over 100 years of invasion by a non-native plant

description: 1. Pathogens can accumulate on invasive plants over time, which could lead
 to population declines. The time required for these dynamics to occur is
 unknown and seldom addressed. Furthermore, no study has assessed
 plant-soil feedback while characterising plant pathogen and mutualist root
 fungal communities in the context of invasion time. 2. We used a
 plant-soil feedback study and 454 pyrosequencing to investigate pathogen
 accumulation over 100 years on a highly invasive plant in eastern North
 America that shows localised declines, Vincetoxicum rossicum
 (Apocynaceae). 3. We collected soil from five sites representing each of
 four invasion periods of V. rossicum across Ontario, Canada (old, ~100
 years; intermediate, 50-60 years; young, &lt;12 years; and uninvaded),
 and grew V. rossicum in these soils in a glasshouse study. Our hypothesis
 was that plants grown in soils invaded for longer periods of time would
 experience less positive feedbacks compared to those grown in more
 recently invaded or uninvaded soils. We collected roots of V. rossicum
 from the invasion periods and performed 454 pyrosequencing targeting
 fungi. We hypothesised that the abundance and richness of fungi that are
 known plant pathogens would be higher in roots from older invasions
 compared to more recent invasions. 4. Contrasting with our hypothesis, V.
 rossicum experienced overall growth promotion due to soil biota,
 regardless of invasion period. Vincetoxicum rossicum roots were colonised
 by a large number of fungal taxa, including many known plant pathogens or
 mutualistic arbuscular mycorrhizal fungi. However, we found no evidence of
 pathogen accumulation in older invaded sites in terms of species
 composition, richness or abundance. 5. Synthesis: Our consistent results
 in the glasshouse and the field highlight the strength of combining
 high-throughput sequencing data with plant-soil feedback experiments. We
 showed that the roots of Vincetoxicum rossicum (Apocynaceae) were
 colonised by many fungal taxa, but found no evidence for changes in plant
 growth or accumulation of fungal pathogens with longer invasion time. High
 pathogen loads may not lead to concurrent declines in invasive plants.
 Plant invasions, as demonstrated by V. rossicum, may be unpredictable in
 their ability to accumulate pathogens capable of leading to population
 declines.
description: Fungal
 colonisationThis file contains data
 for roots hyphal colonisation, based on roots that have been cleared and
 stained with ink-vinegar. Metadata included.Pathogen
 tableThis file contains
 records for each genus identified by 454 and whether they have been
 recorded as a pathogen. Metadata included.Plant dry
 weightsThis file contains the
 dry weights of roots, shoots, and pods. These data were used in the models
 to assess V. rossicum biomass growing in soils from different invasion
 periods. Metadata included.Site by species matrix
 from 454 pyrosequencingThis file contains the
 site by species matrix of the Genome Quebec pyrosequencing data. Metadata
 provided.Reanalysis of Day et al.
 (2015) using FUNGuildContains data from Day
 et al. (2015) where potential plant pathogens were determined by FUNGuild
 to provide a comparison against the manual literature searches done in the
 original analysis.Data_funguild_reanalysis_Day.xlsx