cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/6h3kj-0vk07
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-9/3dimage_68955/</p>12jan14_C107_1_pNL_DN_AT2BT10002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-9/3dimage_68955/12jan14_C107_1_pNL_DN_AT2BT10002_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT2BT10002.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-9/rawdata/12jan14_C107_1_pNL_DN_AT2BT10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT2BT10002_full.tiff,  file associated to 3D Image #68955, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-9/file_16068/12jan14_C107_1_pNL_DN_AT2BT10002_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w3a83-et016
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-20/3dimage_68887/</p>12jan06_c105_1_4ADNCos7_AT3BT10003_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-20/3dimage_68887/12jan06_c105_1_4ADNCos7_AT3BT10003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3BT10003.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-20/rawdata/12jan06_c105_1_4ADNCos7_AT3BT10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3BT10003_full.tiff,  file associated to 3D Image #68887, 1.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-20/file_16029/12jan06_c105_1_4ADNCos7_AT3BT10003_full.tiff"
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DOI: 10.22002/tk4aa-18469
title: COS7

description: Cos7 - not treatment, no transfection
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cells frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and plunge frozen</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-16/3dimage_69029/</p>12jan20_C106_2_COS7neg_AT1BT1_0027_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-16/3dimage_69029/12jan20_C106_2_COS7neg_AT1BT1_0027_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0027.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-16/rawdata/12jan20_C106_2_COS7neg_AT1BT1_0027.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0027_full.tiff,  file associated to 3D Image #69029, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-16/file_16097/12jan20_C106_2_COS7neg_AT1BT1_0027_full.tiff"
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DOI: 10.22002/p40kt-p8844
title: Pseudoalteromonas Iuteoviolacea MP123

description: P Iuteo DvipABhcpI Dbact2
description: <strong>Tilt Series Date:</strong> 2013-06-23</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> on plate</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-23-9/rawdata/</p>2013_06_23_mp123_0013.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-23-9/rawdata/2013_06_23_mp123_0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_06_23_mp123_0013_2k_1.rec,  Reconstruction (Pixel Size 1.97 nm), 347.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-23-9/3dimage_28882/2013_06_23_mp123_0013_2k_1.rec"
        > <i class="download icon"></i> Download </a></p>

        mpa2013-06-23-9_slicer19091.png,  large irregularly shaped REZ with visible flow lines .  nucleoid .  -andrew, 532.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/mpa2013-06-23-9_slicer19091.png"
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DOI: 10.22002/wzmpm-4jp98
title: HeLa

description: Synchronized, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2014-01-05</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> With Doug Mckay and Katherine Ullman</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA NUP 153+ Thymidine 2mM. 14 hour after release</p>
  rra2014-01-05-20_slicer12790.jpg: mitochondrial DNA
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-05-20/rawdata/</p>30004.mrc,  Tilt Series (Pixel Size 0.6 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-05-20/rawdata/30004.mrc"
        > <i class="download icon"></i> Download </a></p>

        30004_part121_8.rec,  Reconstruction (Pixel Size 2.4 nm), 156.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-05-20/3dimage_50383/30004_part121_8.rec"
        > <i class="download icon"></i> Download </a></p>

        30004_full.rec,  Reconstruction (Pixel Size 0.0 nm), 51.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-05-20/3dimage_50547/30004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x4a32-nzn55
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-16</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-8/3dimage_68985/</p>12jan16_pNLDN_AT1BT10015_part122_6.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-8/3dimage_68985/12jan16_pNLDN_AT1BT10015_part122_6.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan16_pNLDN_AT1BT10015.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-8/rawdata/12jan16_pNLDN_AT1BT10015.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan16_pNLDN_AT1BT10015_part122_6.tiff,  file associated to 3D Image #68985, 1.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-8/file_16077/12jan16_pNLDN_AT1BT10015_part122_6.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dyzd7-wr747
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-36/3dimage_68919/</p>12jan06_c105_1_4ADNCos7_AT3man4_full.rec,  Reconstruction (Pixel Size 2.0 nm), 743.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-36/3dimage_68919/12jan06_c105_1_4ADNCos7_AT3man4_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3man4.st,  Tilt Series (Pixel Size 0.5 nm), 512.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-36/rawdata/12jan06_c105_1_4ADNCos7_AT3man4.st"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3man4_full.tiff,  file associated to 3D Image #68919, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-36/file_16045/12jan06_c105_1_4ADNCos7_AT3man4_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hpdt0-j2q55
title: Ori5 MA

description: Raw data files of Ori5 MA
description: <strong>Tilt Series Date:</strong> 2014-02-12</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Methylomicrobium alcaliphilum</p>
<strong>Strain:</strong> 20Z-ori5</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Marina Kalyuzhnaya @ Univ. Washington provided the culture</p>
<strong>Purification / Growth Conditions / Treatment:</strong> P-medium, 3% NaCl, supplemented with 0.2% methylacetate (3rd transfer from methyalcetate plate)</p>
  scy2014-02-12-11_slicer3937.jpg: intra-cytoplasmic membrane arrays; surface proteins; granules;
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-11/3dimage_42645/</p>at111_1k.rec,  Reconstruction (Pixel Size 1.92 nm), 498.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-11/3dimage_42645/at111_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas10011.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-11/rawdata/atlas10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10011_part121_106.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-11/3dimage_38801/atlas10011_part121_106.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/spgh8-nvd15
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-16</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-3/3dimage_68975/</p>12jan16_pNLDN_AT1BT10008_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-3/3dimage_68975/12jan16_pNLDN_AT1BT10008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan16_pNLDN_AT1BT10008.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-3/rawdata/12jan16_pNLDN_AT1BT10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan16_pNLDN_AT1BT10008_full.tiff,  file associated to 3D Image #68975, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-3/file_16073/12jan16_pNLDN_AT1BT10008_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3scjx-jy224
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-41/3dimage_69080/</p>12jan20_C110_3_pNL_INFalpha_AT1BT10017_full.rec,  Reconstruction (Pixel Size 2.0 nm), 737.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-41/3dimage_69080/12jan20_C110_3_pNL_INFalpha_AT1BT10017_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_3_pNL_INFalpha_AT1BT10017.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-41/rawdata/12jan20_C110_3_pNL_INFalpha_AT1BT10017.st"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_3_pNL_INFalpha_AT1BT10017_full.tiff,  file associated to 3D Image #69080, 993.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-41/file_16117/12jan20_C110_3_pNL_INFalpha_AT1BT10017_full.tiff"
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DOI: 10.22002/pf28g-z2d56
title: Myxococcus xanthus JK328

description: Bactofilin BacMNOP knockout strain. Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-07-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-07-22-62_slicer6126.jpg: unknown tube
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-62/rawdata/</p>07222013_Janelia_Myxo_JK328_20004.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        07222013_Janelia_Myxo_JK328_20004_part131_5.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
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        07222013_Janelia_Myxo_JK328_20004_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 258.4 MB  
        
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