cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2006.1779
title: Struggling to get connected : the process of maternal attachment to the preterm infant in the neonatal intensive care unit

description: Hospitalization of preterm infants in the NICU is crisis for mothers in developing an attachment process. The purpose of this grounded theory study was to explore how mothers in Thailand develop maternal attachment to infants born preterm, and requiring NICU hospitalization. Fifteen Thai mothers whose preterm infants had birth weights less than 1,500g, without congenital anomalies, and experienced mechanical ventilation were interviewed and audiotaped. Four mother-preterm infant dyads interaction were observed and videotaped. The audiotape and videotape were transcribed for analysis. Textual data were analyzed through the constant comparative method developed by Strauss and Corbin. Findings indicate the basic social process of maternal attachment was "Struggling to Get Connected" through the crisis circumstance of preterm birth, composed of 4 phases of establishing the connections, disrupting of the connection, resuming to get connected, and becoming connected. The movement of actions/interactions of maternal attachment to the preterm infants in these phases depended on having concern for the baby, adjusting emotionally to the crisis, supporting connections, life experience, and health care system facilitating. Discussion included the cultural context. Understanding this process was valued in clinical practice and nursing education. Future direction of qualitative and quantitative investigation was recommended.
DOI: 10.14457/cu.the.2006.1780
title: Estimation of mixing height using surface meteorological data in Thailand

description: The height of the atmospheric boundary layer (ABL) or the mixing height (MH) is a key parameter of air pollution dispersion model. Two basic possibilities for the practical determination of the MH are its derivation from measurements of profile data, and its parameterization from routine meteorological data using simple models. In Thailand the radiosondes have been applied for MH only 5 stations (Bangkok, Chiang Mai, Ubonratchathani, Phuket and Songkhla station), so the MH is not normally available for the other stations. The objectives of this study to develop and apply empirical parameters that measurement in Thailand to simple model, which is used to estimate MH from surface meteorological data. The model require a few routine meteorological data of air temperature, relative humidity, wind speed and total cloud cover, and its out put is in term of hourly daytime MH and nighttime MH. The most important methods of comparison between MH obtained from model and MH obtained from upper air profile measurements had been tested on data set from Observatory for Atmospheric Radiation Research at Srisamrong, Sukhothai and data collected from Thai Meteorological Department (Bangkok, Chiang Mai, Ubonratchathani, and Phuket station) during 20 April to 6 June 2003. The results of estimation MH were tested accuracy by factor of two (FT). It found that the percentage of acceptable was about 60 – 80 %. Then, we can conclude that the simple model, which is applied the empirical parameters that measurement in Thailand can be used to estimate MH in other areas with absence of appropriate upper air sounding data.
DOI: 10.7295/w9cil50753
title: CIL:50753, Drosophila melanogaster, L1 larval brain
DOI: 10.14457/cu.the.2006.1781
title: Antinociceptive activity of the ethanolic extract from Thai propolis

description: Propolis (bee glue) is a resinous substance which contains a complex mixture of secondary metabolites, produced by honeybees. It has long been used in many countries for the management of several diseases. In these studies, we initially determined the analgesic property of a range of the ethanolic extract of Thai propolis (ETP) doses in the mouse hot-plate test. Hot-plate latencies (cut-off 45 sec) were determined in male ICR mice prior to the intraperitoneal (i.p.) administration of corn oil, 0.9% normal saline solution (NSS), morphine (MO: 10 mg/kg), indomethacin (IND: 150 mg/kg) or various doses of ETP (12.5, 25, 50, 100, 200, 400, 800 mg/kg). Hot-plate latencies were subsequently determined at 15, 30, 45, 60, 90, 120, 240 min. The percent maximum possible effect (%MPE) was calculated and used in determination of the area of analgesia (%MPE-min). ETP in doses of 50-800 mg/kg produced a significant dose-related analgesic response. ETP (200 mg/kg) produced analgesic response that was naloxone and naltrexone-sensitive suggesting opioid-mediated mechanism. In mouse tail-flick analgesia test, tail-flick latencies (cut-off 4 sec) were determined prior to the i.p. administration of corn oil, NSS, MO, IND or various doses of ETP (12.5-800 mg/kg) and were subsequently determined at 7 intervals over a 4-hr period. ETP in doses of 25-800 mg/kg produced a dose-dependent analgesic response. Studies then determined the analgesic effect of ETP using the formalin-induced nociception test. After the i.p. administration of corn oil, NSS, MO, IND or various doses of ETP (50-800 mg/kg), 2.5% solution of formalin was administered into subplantar area of the right hind paw. The number of licks were subsequently determined at the first 0–5 min, and the following 25–30 min after formalin administration. ETP dose of 200 mg/kg produced analgesic response during the first phase and ETP doses of 200 mg/kg or higher produced a significant dose-related analgesic response during the second phase. ETP doses of 200-800 mg/kg i.p. failed to produce motor impairment compared to vehicle control in the rota-rod test. Taken together these results demonstrate that the ethanolic extract of Thai propolis produced analgesic effect that was dose-dependent in all analgesic testing models without altering motor performance and mechanism of actions seem to be related to the opioid receptors.
DOI: 10.14457/cu.the.2006.1782
title: Measuring object-oriented software maintainbility in design phase using structural complexity and aesthetic metrics

description: Qualities of software design heavily affect on qualities of software ultimately developed. One of the claimed advantages of object-oriented paradigm is the ease of maintenance. Assessing maintainability in design phase will help software designers to decide that the design of software should be altered or not. This will help reducing cost of software maintenance in later phases. This research presents a controlled experiment carried out to investigate whether structural complexity and aesthetic metrics can be indicators of class and sequence diagrams maintainability and to establish maintainability models from both metric sets. Maintainability models are constructed by using three techniques: Discriminant analysis, Decision tree and MLP neural network in order to find the best one among these models. The preliminary result shows that the maintainability models constructed by applying MLP neural network are the best models in terms of the model accuracy. Moreover, this research constructs an automated tool for predicting maintainability using the maintainability models and also proposes a new set of structural complexity metrics for measuring maintainability from class diagrams.
DOI: 10.7295/w9cil50755
title: CIL:50755, Drosophila melanogaster, L1 larval brain
DOI: 10.7295/w9cil50756
title: CIL:50756, Drosophila melanogaster, L1 larval brain
DOI: 10.14457/cu.the.2006.1783
title: The Thai labour unions : identifying internal constraints to effective representation of members' interests in the workplace and in society

description: Workers around the globe seem to share the same constant up-hill struggle for dignity, justice, fairness and a decent way of life. Trade unions have been and continue to be the pivotal institution for working class people to advance their struggle for themselves, their families, and their communities. The past few decades have borne witness to ever-greater challenges to workers and their unions by those who believe they benefit from limiting the power and effectiveness of unions. Consequently, unions all over the world, including in America and Thailand, have faced setbacks in recent decades. There have however been developments that have shown real promise in turning around the setbacks organized labor has been facing. One such development is the organizing model of unionism.This research is focused on the Thai trade union movement. It is not an elaboration of the many hurdles the movement faces from employers, the government or the law. It is instead an examination of what capacities Thai unions have to adopt and implement the organizing model of unionism.This research seeks to examine what internal constraints Thai unions need to address as a prerequisite to adopting the organizing model. To that end, the research is chiefly concerned with examining two key resources needed to adopt the model: financial resources (dues) and organizing staff. The research reveals that Thai unions currently lack both the financial resources and organizing staff to adopt the organizing model.
DOI: 10.14457/cu.the.2006.1784
title: Renal function and oxidative stress following gentamicin induced renal injury in rats treated with erythropoietin, iron and vitamine E

description: Effects of erythropoietin, iron, and vitamin E on renal function and oxidative stress induced by gentamicin in rats were investigated. Rats were divided into 5 groups; group 1 (control: NSS injection on day 1-12); group 2 gentamicin injection ( 100 mg/kg s.c. on day 5-12 ); group 3 gentamicin plus erythropoietin (EPO) (gentamicin 100 mg/kg s.c., EPO 100 i.u./kg s.c. on day 5-12) group 4 gentamicin plus EPO and iron (iron 500 mg/kg i.p. on day 4, gentamicin 100 mg/kg, EPO 100 i.u./kg s.c. on day 5-12 and) ; group 5 gentamicin plus EPO, iron, and vitamin E (vitamin E 250 i.u./kg orally on day 1-3, iron 500 mg/kg i.p. on day 4, gentamicin 100 mg/kg, EPO 100 i.u./kg s.c. iron 500 mg/kg i.p., on day 5-12). The results showed that plasma creatinine and BUN concentrations of rats in group 2,3,4, and 5 were significantly increased while glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) decreased. The fractional excretion of Na+ and K+ and protein excretion were higher in groups 2,3,4 and 5. Group 3 with EPO did not improve GFR and ERPF. However, kidney GSH was decreased compared with group 2. Rats receiving iron showed no changes in renal function and oxidative stress compared with group 3. However, supplement with vitamin E in group 5 caused higher ERPF. In conclusion, gentamicin induced nephrotoxicity by causing severe damage of both glomerulus and renal tubular cells with alteration of oxidative stress. EPO and iron did not alter renal function but vitamin E supplementation could improve blood flow to the kidney.
DOI: 10.7295/w9cil50758
title: CIL:50758, Drosophila melanogaster, L1 larval brain
DOI: 10.14457/cu.the.2006.1785
title: Nicotine and polycyclic aromatic hydrocarbons modulate junctional proteins and paracellular permeability of rat cerebral endothelial cells

description: Cigarette smoking contributes to the development of several neurological disorders associated with blood-brain barrier (BBB) dysfunction. However, very little is know about the effect of smoking on the BBB. Our work was aimed on the effect of major cigarette smoke components on rat cerebral endothlial cells (CECs). Western blot analysis was used to investigate whether nicotine and polycyclic aromatic hydrocarbons (PAH), cigarette smoke components, had an effect on junctional protein expression. The results revealed that the rat CECs exposed to 10 muM nicotine 24 h significantly decreased cadherin and ZO-1 protien expression in triton X-100 (Tx-100) soluble fraction while no change was observed either in the case of phenanthrene or 1-methylanthracene exposure at the concentration of 30muM. Immunostaining experiments were used to determine the possible rearrangment in the subcellular distribution of cadherin and ZO-1. A disruption in the continuity of cadherin and ZO-1 staining along cell-cell contacts were observed, which was correlated with the observation obtained by Weatern blot analysis. No change of protein distribution after the treatment with either phenanthrene or 1-methylanthracene was observed. Due to the observation of a significant change in ZO-1 protein expression after nicotine exposure, protein-protein interactions within the rat CECs were further investigated using co-immunoprecipitation assay. The results showed that protein-protein interactions between occludin and ZO-1 decreased after nicotine exposure. To assess the potential damaging effect of cigarette smoke components on the barrier function, transendothelial electrical resistance (TEER) was measured in a coculture model using rat CECs and astrocytes. Although nicotine decreased ZO-1 protein expression and localization of junctional proteins, the barrier permeability evaluated by TEER measurements did not change in our study. The barrier permeability was also not affected by the treatment with either phenanthrene or 1-methylanthracene. There are several reports showing that oxidative stress is implicated in pathological processes of the central nervous system. Therefore, the potential cumulative effect of oxidative stress and nicotine on the BBB permeability was investigated. Immunostaining analysis revealed that co-treatment of the rat CECs with 10 muM nicotine in the presence of 10 muM 2,3-dimethoxy-1,4-naphthoquinone (DMNQ) disrupted the continuity of ZO-1 staining along the cell-cell contacts, which was more pronounce compared to DMNQ or nicotine treatmemt alone. Our results revealed that nicotine treatmemt under oxidative stress for 24 h increased the transendothelial permesbility. In conclusion, nicotine significantly affects the junctional complex of the rat CECs by changing protein expression and protein localization of cadherin and ZO-1. The damaging effects are enhanced by oxidative stress, which can further disturb the function of the BBB.
DOI: 10.14457/cu.the.2006.1786
title: Protein engineering of bacterial chitinase for N-acetylchitooligosaccharide production

description: In this study, genetic engineering and protein engineering techniques were used to modify bacterial chitinase for the production of N-acetylglucosamine and/or N-acetylchitooligosaccharide. Beta-chitin and colloidal chitin were used as substrate for sugar production by the chitinase derivatives. Bacillus licheniformis and Serrtia sp. chitinases were selected for studying of the oligosaccharide production. Chitinase A1 (ChiA1), chitinase 60 (Chi60) and chitinase B (ChiB) were cloned, expressed, partially purified and studied. ChiA1, Chi60 and ChiB have both of exo- and endo-type modes for hydrolysis depending on the type of the substrate. The different hydrolysis mode of enzymatic hydrolysis can be used to prepare NAG or N-acetylchitooligosaccharide. Exo-chitinase can be use for the production of N-acetylchitobiose while endo-chitinase can be used for the production N-acetylchitooligosaccharide. The engineered chitinase with deletion and insertion of the accessory domains in ChiA1 and Chi60 was constructed and characterized. The chitinase derivatives with high endo-type activity should be a good candidate for oligosaccharide production. The homologous recombination derivatives between chitinase 60 and chitinase B was constructed and characterized. Interestingly, the chimeric chitinase (RecSK-1) can hydrolyze pNP-NAG [subscript 2] and NAG [subscript 2] to produce monomer of NAG. In addition, the chimeric enzyme can be useful for the preparation of N-acetyl-D-glucosamine from chitin. Chi60 mutant, Chi60W33F/W245F, was used to prepare N- N'-diacetylchitobiose from beta-chitin and the product was separated by dialysis. The amounts of chitobiose from dialysis method yielded 57% (w/w) purity. After 6 days of incubation, the HPLC yields of NAG and NAG [subscript 2] from the hydrolysis of g beta-chitin were 85 mg and 565 mg, respectively, representing 9% and 57% (w/w). In addition, the ChiA1 ChBD was used to prepare oligosaccharide from colloidal chitin. In the condition used, chitotetraose can be prepared.
DOI: 10.14457/cu.the.2006.1787
title: Prevalence and van gene of vancomycin-resistant enterococci isolated from ark shell (arca granulosa) in Thailand

description: Prevalence of vancomycin resistant enterococci (VRE) in Thailand had been studied in farm animals, food of animal origins, domesticated animals and human. However, VRE in environment has not been reported in Thailand. Therefore this study was used ark shells which cultivated from the Gulf of Thailand as biological marker of VRE in the environment. They were filter feeders which could concentrate materials presented in water in their digestive tracts. Ark shell samples were collected from August 2005 to August 2006. Samples were pooled and individual counts; pooled of ark shell from same source approximately 10 shells which give total weight 25g as one sample, and individual shell from one source as one sample. All samples were screened for VRE by selective media contained 6 g of vancomycin per mL. Individual ark shell samples were found 0.36% of low level resistant VRE and all 5 isolates (100%) were classified as E.gallinarum. Pooled ark shell samples were found 26 isolates (4.3%) and classified as E.faecium 15 isolates (57.7%), E.faecalis 6 isolates (23.1%), E.gallinarum 3 isolates (11.5%) and E.casseliflavus 2 isolates (7.7%). Antimicrobial susceptibility test had been performed by using agar dilution method for six antibiotics; vancomycin (VN), ampicillin (AP), cholramphenicol (CHPC), erythromycin (ET), tetracycline (TE) and tylosin (TS) and E-test for teicoplanin (TP). All of 5 VRE isolated from individual ark shell samples were low level resistance (MIC = 8 g/ml) to VN and sensitive to all of other antibiotics. All of VRE isolated from from pooled ark shell samples were low level resistance to VN and sensitive to TP. E.faecium 15 isolates were resistance to AP, CHPC, TE and TS 13.3% and resistance to ET 33.3%. E.faecalis 6 isolates were susceptible to AP and CHPC and resistance to ET, TE and TS 16.7%. E.gallinarum were susceptible to AP and resistance to CHPC and TS 33.3% and resistance in ET and TE 66.7% and one from two of E.casseliflvus was resistance to only antibiotics; ET. Detection of van gene from all of low level resistance VRE by polymerase chain reaction (PCR) were found gene vanC1 in all of E.gallinarum isolates and vanC2/C3 in all of E.casseliflavus isolates. The low prevalence of VRE found in this study which showed low level resistance to vancomycin and susceptible to teicoplanin. Therefore VRE should not be a public health threat in Thailand.
DOI: 10.14457/cu.the.2006.1788
title: An unsupervised learning algorithm for robust clustering and estimating the feasible number of clusters

description: Data clustering is a discovery process that groups a set of data such that the data points in the same cluster are as similar as possible and the data points of different clusters are as dissimilar as possible. Existing clustering algorithms, such as single-link clustering, k-means, CURE, and CSM are designed to find clusters based on pre-defined parameters specified by users. These algorithms can breakdown if the choice of parameters is incorrect with respect to the data set being clustered. Most of these algorithms work very well for compact and hyperspherical clusters. In this dissertation, the new hybrid clustering algorithm called “Self-Partition and Self-Merging” (SPSM) is proposed. The SPSM algorithm partitions the input data set into several subclusters in the first phase, and then removes the noisy data and the noisy subclusters in the second phase. In the third phase, the dense subclusters are continuously merged to form the larger clusters based on the inter-distance and intra-distance criteria. From the experimental results, the SPSM algorithm is very efficient to handle the noisy data set. Moreover, the SPSM algorithm is able to cluster the data sets of arbitrary shapes and different density very efficiently, tolerate to noise, and provide better clustering results than the existing clustering algorithms. The computational complexity of the SPSM algorithm is O(N [superscript 2]), where N is the number of data points.
DOI: 10.6084/m9.figshare.11858880.v1
title: Additional file 6 of Membrane and synaptic defects leading to neurodegeneration in Adar mutant Drosophila are rescued by increased autophagy

description: Additional file 6. Excel sheet containing source data file for Figure 1A ,1B ,1C.1D ,4A,4D,4E,6A,6F,6G,6H and 6I.
DOI: 10.14457/cu.the.2006.1789
title: Window-based lerning for overcomplete independent component analysis

description: The blind source separation (BSS) or independent component analysis (ICA) is a statistical technique for the separation of hidden source signals from observed signals in an unknown mixing system. This dissertation concerns the separation problem, where the number of sources (n) is greater than the number of observed signals (m). This situation is called overcomplete. Many existing algorithms are designed to identify the column vectors of the mixing matrix, basis components, which point toward the directions of independent components. However, most approaches assume that the number of sources mixed in the observed signals is known. This dissertation presents a new method to identify the mixing matrix without prior assumption on the number of sources. The proposed algorithm uses a window search length algorithm to identify the information index for preliminary filtering all relevant points clustered along the basic independent components. Then, the perturbed mean shift algorithm with entropy measure is applied to enhance the actual basic independent components. Finally, source signals are recovered by the minimum l1-norm method. From the experimental results on the speech signals from TIMIT database, the proposed algorithm is able to estimate the mixing matrix and the estimated source number is also given. The difference between the actual mixing matrix and the estimated mixing matrix using AMDI value of the proposed algorithm is less than that of standard k-mean method, AICA method, Yuanqing Li et al. ‘s algorithm, and Qv Lv et al. ‘s algorithm. Moreover, the algorithm can perform in noisy environments.