cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21755579
title: Insulin replacement prevents the acquisition but not the expression of morphine-induced conditioned place preference in streptozotocin-induced diabetic rats

description: Abstract Insulin receptors have distributed in all brain regions, including the nucleus Accumbens (NAc), and where is implicated in the reward properties of drugs. It is well known that insulin signaling can regulate dopamine release. Therefore, in the present study, we tried to examine the effect of insulin replacement on the acquisition and expression of morphine-induced conditioned place preference (CPP) in diabetic rats. Forty-eight male Wistar rats were divided into two non-diabetic (Naïve) and diabetic groups rendered by a single injection of streptozotocin (STZ). These groups separately received insulin (10U/kg) or saline (1 ml/kg) one hour prior to morphine administration (5mg/kg;s.c.) during conditioning days (acquisition phase) or post-conditioning day (expression phase) in the CPP paradigm. In this paradigm, conditioning score (CS) and locomotion activity were recorded by Ethovision. The STZ-induced diabetic rats displayed higher CS compared to naïve rats (P&lt;0.05). This effect was abolished in all diabetic rats that received insulin during conditioning days but not the expression phase. This study has provided evidence that insulin plays a modulatory role in morphine-induced CPP, and insulin replacement during the acquisition phase could reduce the rewarding properties of morphine in diabetes conditions through a possible modulating effect on dopamine release in the NAc region.
DOI: 10.6084/m9.figshare.21755579.v1
title: Insulin replacement prevents the acquisition but not the expression of morphine-induced conditioned place preference in streptozotocin-induced diabetic rats

description: Abstract Insulin receptors have distributed in all brain regions, including the nucleus Accumbens (NAc), and where is implicated in the reward properties of drugs. It is well known that insulin signaling can regulate dopamine release. Therefore, in the present study, we tried to examine the effect of insulin replacement on the acquisition and expression of morphine-induced conditioned place preference (CPP) in diabetic rats. Forty-eight male Wistar rats were divided into two non-diabetic (Naïve) and diabetic groups rendered by a single injection of streptozotocin (STZ). These groups separately received insulin (10U/kg) or saline (1 ml/kg) one hour prior to morphine administration (5mg/kg;s.c.) during conditioning days (acquisition phase) or post-conditioning day (expression phase) in the CPP paradigm. In this paradigm, conditioning score (CS) and locomotion activity were recorded by Ethovision. The STZ-induced diabetic rats displayed higher CS compared to naïve rats (P&lt;0.05). This effect was abolished in all diabetic rats that received insulin during conditioning days but not the expression phase. This study has provided evidence that insulin plays a modulatory role in morphine-induced CPP, and insulin replacement during the acquisition phase could reduce the rewarding properties of morphine in diabetes conditions through a possible modulating effect on dopamine release in the NAc region.
DOI: 10.6084/m9.figshare.21755582
title: Morphometric studies suggest taxonomic changes in a species complex in Chromolaena (Asteraceae, Eupatorieae, Praxelinae)

description: ABSTRACT The Chromolaena congesta complex (Asteraceae, Eupatorieae) presents a difficult challenge for biodiversity researchers due to the intertwined connections of its species and the difficulty of establishing morphological boundaries. In this study, we aimed to use morphometric analyses to evaluate the delimitation of the taxa belonging to the C. congesta complex, identify informative morphological traits and to understand the identity of several “atypical” specimens morphologically related to the complex. To achieve this, we used cluster and principal component analysis to evaluate 50 morphological traits from a total of 210 specimens throughout the geographic distribution of the species complex. We found support for the recognition of at least six species - Chromolaena ascendens, C. gentianoides, C. hirsuta, C. latisquamulosa, C. rhinanthacea and C. squarrulosa - according to a phenetic species concept and to corroborate the restoration of three species from synonymy. Furthermore, our results provide a morphological recircumscription of C. congesta and C. elliptica and indicate two taxonomic novelties, including a new combination and a new species. Despite the answers provided by morphology, many taxonomic issues remain to be solved, and further studies with other types of evidence should be carried to contribute towards a more stable classification.
DOI: 10.6084/m9.figshare.21755582.v1
title: Morphometric studies suggest taxonomic changes in a species complex in Chromolaena (Asteraceae, Eupatorieae, Praxelinae)

description: ABSTRACT The Chromolaena congesta complex (Asteraceae, Eupatorieae) presents a difficult challenge for biodiversity researchers due to the intertwined connections of its species and the difficulty of establishing morphological boundaries. In this study, we aimed to use morphometric analyses to evaluate the delimitation of the taxa belonging to the C. congesta complex, identify informative morphological traits and to understand the identity of several “atypical” specimens morphologically related to the complex. To achieve this, we used cluster and principal component analysis to evaluate 50 morphological traits from a total of 210 specimens throughout the geographic distribution of the species complex. We found support for the recognition of at least six species - Chromolaena ascendens, C. gentianoides, C. hirsuta, C. latisquamulosa, C. rhinanthacea and C. squarrulosa - according to a phenetic species concept and to corroborate the restoration of three species from synonymy. Furthermore, our results provide a morphological recircumscription of C. congesta and C. elliptica and indicate two taxonomic novelties, including a new combination and a new species. Despite the answers provided by morphology, many taxonomic issues remain to be solved, and further studies with other types of evidence should be carried to contribute towards a more stable classification.
DOI: 10.6084/m9.figshare.21624478.v1
title: Protocol for the in vitro rearing of Frieseomelitta varia workers (Hymenoptera: Apidae: Meliponini)

description: ABSTRACT In vitro rearing protocols already established for honeybees are currently being adapted to assess the risk of pesticides, and to conduct comparative developmental biology studies on stingless bees. However, differences in critical life-history traits (development time and the type of larval nutrition leading to caste differentiation process) among social bees require the development of an in vitro rearing protocol for each species and caste. We generated a protocol to produce workers of Frieseomelitta varia (Lepeletier, 1836), a non-endangered and highly eusocial pollinator species with wide geographical distribution. We tested the viability of using either the eggs or the first instar larvae as the starting point for in-vitro transfer. In vitro rearing was performed in acrylic plates at 30 °C and 99% relative humidity during the larval feeding phase. The humidity was subsequently reduced to 75% during the following days of development. The experimental larvae were offered either 25 µL or 27 µL of larval food. The development time, emergence and mortality rates, and morphological parameters of the emerged workers were assessed. In the process of validating the protocol, the adults that emerged after in vitro rearing were compared with colony-reared adults. In our results, 27 µL of larval food allowed 90% of workers to emerge. No significant differences were found between the emerging workers reared in vitro and those reared in the colony. The described protocol is a useful method for rearing F. varia workers in vitro, which can be used for diverse types of experimental approaches.
DOI: 10.6084/m9.figshare.21624478
title: Protocol for the in vitro rearing of Frieseomelitta varia workers (Hymenoptera: Apidae: Meliponini)

description: ABSTRACT In vitro rearing protocols already established for honeybees are currently being adapted to assess the risk of pesticides, and to conduct comparative developmental biology studies on stingless bees. However, differences in critical life-history traits (development time and the type of larval nutrition leading to caste differentiation process) among social bees require the development of an in vitro rearing protocol for each species and caste. We generated a protocol to produce workers of Frieseomelitta varia (Lepeletier, 1836), a non-endangered and highly eusocial pollinator species with wide geographical distribution. We tested the viability of using either the eggs or the first instar larvae as the starting point for in-vitro transfer. In vitro rearing was performed in acrylic plates at 30 °C and 99% relative humidity during the larval feeding phase. The humidity was subsequently reduced to 75% during the following days of development. The experimental larvae were offered either 25 µL or 27 µL of larval food. The development time, emergence and mortality rates, and morphological parameters of the emerged workers were assessed. In the process of validating the protocol, the adults that emerged after in vitro rearing were compared with colony-reared adults. In our results, 27 µL of larval food allowed 90% of workers to emerge. No significant differences were found between the emerging workers reared in vitro and those reared in the colony. The described protocol is a useful method for rearing F. varia workers in vitro, which can be used for diverse types of experimental approaches.
DOI: 10.6084/m9.figshare.21747701
title: Questionnaire Responses.xlsx

description: The questionnaire responses related to the factors of using e-learning system
DOI: 10.6084/m9.figshare.21747701.v1
title: Questionnaire Responses.xlsx

description: The questionnaire responses related to the factors of using e-learning system
DOI: 10.6084/m9.figshare.14277827
title: Cancer and Cardiovascular Diseases during the COVID-19 Pandemic

description: Abstract The challenges that the COVID-19 pandemic cretead to the healthcare system have made it necessary to adapt routines and services, with the objectives of controlling the spread of the virus and preserving health. Safe and correct management of patients in risks groups, such as elderly patients, patients with cardiovascular diseases, and patients with cancer, has become even more important. Thus, cardio-oncology has gained a new dimension, with the aim of adapting to patients’ needs during the pandemic by restructuring the system of care in a manner that offers quality and safety in healthcare.
DOI: 10.6084/m9.figshare.14277827.v1
title: Cancer and Cardiovascular Diseases during the COVID-19 Pandemic

description: Abstract The challenges that the COVID-19 pandemic cretead to the healthcare system have made it necessary to adapt routines and services, with the objectives of controlling the spread of the virus and preserving health. Safe and correct management of patients in risks groups, such as elderly patients, patients with cardiovascular diseases, and patients with cancer, has become even more important. Thus, cardio-oncology has gained a new dimension, with the aim of adapting to patients’ needs during the pandemic by restructuring the system of care in a manner that offers quality and safety in healthcare.
DOI: 10.6084/m9.figshare.21755585
title: ACTN3 GENE POLYMORPHISM AND FACTORS ASSOCIATED WITH FUNCTIONAL PERFORMANCE IN ELDERLY WOMEN ASSISTED IN PRIMARY HEALTH CARE

description: ABSTRACT Objective: To analyze potential influences of the R/X genetic polymorphism of the ACTN3 gene, as well as of anthropometric and metabolic characteristics on the functional performance of elderly women assisted in primary health care. Method: One hundred and forty-one elderly women were assessed in terms of anthropometric, metabolic and functional aspects, in addition to clinical, cognitive and demographic characteristics. Allele and genotype frequencies of ACTN3 gene polymorphism were determined. Results: 141 elderly women (68.30 ± 6.18 years) were evaluated. No significant differences (p &gt; 0.05) were observed between the RR and RX/XX genotypes in the elderly women’s functional performance, anthropometric or metabolic characteristics. The TUG test completion time showed positive correlations with age, body mass index, waist circumference, and fat percentage (s = 0.315; p &lt; 0.001; s = 0.238; p = 0.005; s = 0.174; p = 0.039; s = 0.207; p = 0.014), respectively. Negative correlations were found between the TUG test with absolute handgrip strength (s = - 0.314; p &lt; 0.001) and relative handgrip strength (s = - 0.380; p &lt; 0.001). Conclusion: In our study, there were no influences from ACTN3 gene polymorphisms on the functional performance of the elderly women, which is influenced by other factors.
DOI: 10.6084/m9.figshare.21755585.v1
title: ACTN3 GENE POLYMORPHISM AND FACTORS ASSOCIATED WITH FUNCTIONAL PERFORMANCE IN ELDERLY WOMEN ASSISTED IN PRIMARY HEALTH CARE

description: ABSTRACT Objective: To analyze potential influences of the R/X genetic polymorphism of the ACTN3 gene, as well as of anthropometric and metabolic characteristics on the functional performance of elderly women assisted in primary health care. Method: One hundred and forty-one elderly women were assessed in terms of anthropometric, metabolic and functional aspects, in addition to clinical, cognitive and demographic characteristics. Allele and genotype frequencies of ACTN3 gene polymorphism were determined. Results: 141 elderly women (68.30 ± 6.18 years) were evaluated. No significant differences (p &gt; 0.05) were observed between the RR and RX/XX genotypes in the elderly women’s functional performance, anthropometric or metabolic characteristics. The TUG test completion time showed positive correlations with age, body mass index, waist circumference, and fat percentage (s = 0.315; p &lt; 0.001; s = 0.238; p = 0.005; s = 0.174; p = 0.039; s = 0.207; p = 0.014), respectively. Negative correlations were found between the TUG test with absolute handgrip strength (s = - 0.314; p &lt; 0.001) and relative handgrip strength (s = - 0.380; p &lt; 0.001). Conclusion: In our study, there were no influences from ACTN3 gene polymorphisms on the functional performance of the elderly women, which is influenced by other factors.
DOI: 10.6084/m9.figshare.14277781
title: Quantification of Calcified Coronary Plaques by Chest Computed Tomography: Correlation with the Calcium Score Technique

description: Abstract Background Cardiovascular disease is the leading cause of mortality in the world. Parietal calcifications of the arteries may be visualized and quantified at initial and subclinical states by computed tomography (CT), and expressed as calcium score (CS). It is possible to estimate the prognosis of future cardiovascular events using this score. Objectives To correlate the detection and quantification of the CS obtained by chest CT with that obtained by electrocardiography (ECG)-synchronized cardiac computed tomography (the gold-standard). Method Cross-sectional, descriptive study of 73 consecutive patients in investigation for coronary artery disease who underwent cardiac CT between June 2013 and October 2014. Chest computed tomography and CS protocols were performed in a 64-channel TC scanner. P-values &lt;0.05 were considered statistically significant. Results In the per-patient analysis, after logarithmic transformation, mean CS was 8.7 and 9.4 by the ECG-synchronized method and chest CT, respectively. The prevalence of disease was 49.3% (n=36), with a sensitivity of 97.2% and specificity of 100.0%. There was an excellent correlation between the methods (r= 0.993, p&lt;0.001). In the per-segment analysis, after logarithmic transformation, mean CS was 3.0 and 3.2 by the ECG-synchronized method and chest CT, respectively. The prevalence of disease was 29.5% (n=86), with a sensitivity of 95.3% and specificity of 97.5%. There was an excellent correlation between the methods (r= 0.985, p&lt;0.001). Conclusion ECG-synchronized CT is well correlated with the non-ECG-synchronized CT for CS determination, without statistical difference between the methods. (Arq Bras Cardiol. 2020; 115(3):493-500)
DOI: 10.6084/m9.figshare.14277781.v1
title: Quantification of Calcified Coronary Plaques by Chest Computed Tomography: Correlation with the Calcium Score Technique

description: Abstract Background Cardiovascular disease is the leading cause of mortality in the world. Parietal calcifications of the arteries may be visualized and quantified at initial and subclinical states by computed tomography (CT), and expressed as calcium score (CS). It is possible to estimate the prognosis of future cardiovascular events using this score. Objectives To correlate the detection and quantification of the CS obtained by chest CT with that obtained by electrocardiography (ECG)-synchronized cardiac computed tomography (the gold-standard). Method Cross-sectional, descriptive study of 73 consecutive patients in investigation for coronary artery disease who underwent cardiac CT between June 2013 and October 2014. Chest computed tomography and CS protocols were performed in a 64-channel TC scanner. P-values &lt;0.05 were considered statistically significant. Results In the per-patient analysis, after logarithmic transformation, mean CS was 8.7 and 9.4 by the ECG-synchronized method and chest CT, respectively. The prevalence of disease was 49.3% (n=36), with a sensitivity of 97.2% and specificity of 100.0%. There was an excellent correlation between the methods (r= 0.993, p&lt;0.001). In the per-segment analysis, after logarithmic transformation, mean CS was 3.0 and 3.2 by the ECG-synchronized method and chest CT, respectively. The prevalence of disease was 29.5% (n=86), with a sensitivity of 95.3% and specificity of 97.5%. There was an excellent correlation between the methods (r= 0.985, p&lt;0.001). Conclusion ECG-synchronized CT is well correlated with the non-ECG-synchronized CT for CS determination, without statistical difference between the methods. (Arq Bras Cardiol. 2020; 115(3):493-500)
DOI: 10.6084/m9.figshare.21755586
title: Theophylline attenuates bleomycin-induced oxidative stress in rats: The role of IL-6, NF-κB, and antioxidant enzymes

description: Abstract The purpose of this study was to evaluate the antifibrotic and antioxidant roles of theophylline (Theo), a bioactive compound, in bleomycin (BLM)-induced pulmonary fibrosis in Wistar albino rats. Assigned into 4 groups were 32 Wistar albino rats, comprising the control group (administered 0.9% isotonic saline), BLM group (treated with BLM at a dose of 2.5 mg/kg), BLM+Theo group (treated with Theo at a dose of 75 mg/kg + BLM at a dose of 2.5 mg/kg), and Theo group (treated with Theo at a dose of 75 mg/kg). In the BLM group, a significant decrease was observed in the catalase and glutathione peroxidase enzyme activities, and reduced glutathione (GSH) (p &lt; 0.05, p&lt; 0.05, p&lt; 0.001, respectively), while the malondialdehyde (MDA) levels (p&lt; 0.001) were significantly elevated when compared to the control group. However, the MDA levels in the BLM+Theo group were also significantly higher than in the control group (p&lt; 0.01). Similarly, the GSH levels were significantly higher in the BLM+Theo group than in the BLM group (p&lt; 0.05). The results indicated that Theo reduced the BLM-induced activation of nuclear factor-kappaB (NF-κB) and decreased interleukin-6 (IL-6) levels, together with significant amelioration of the immunohistochemical and histopathological architecture in the lung tissues. It was concluded that the administration of Theo had a positive effect on the GSH level, and activation of NF-κB and IL-6 expression, which were significant proinflammatory markers in the BLM-treated rats.
DOI: 10.6084/m9.figshare.21755586.v1
title: Theophylline attenuates bleomycin-induced oxidative stress in rats: The role of IL-6, NF-κB, and antioxidant enzymes

description: Abstract The purpose of this study was to evaluate the antifibrotic and antioxidant roles of theophylline (Theo), a bioactive compound, in bleomycin (BLM)-induced pulmonary fibrosis in Wistar albino rats. Assigned into 4 groups were 32 Wistar albino rats, comprising the control group (administered 0.9% isotonic saline), BLM group (treated with BLM at a dose of 2.5 mg/kg), BLM+Theo group (treated with Theo at a dose of 75 mg/kg + BLM at a dose of 2.5 mg/kg), and Theo group (treated with Theo at a dose of 75 mg/kg). In the BLM group, a significant decrease was observed in the catalase and glutathione peroxidase enzyme activities, and reduced glutathione (GSH) (p &lt; 0.05, p&lt; 0.05, p&lt; 0.001, respectively), while the malondialdehyde (MDA) levels (p&lt; 0.001) were significantly elevated when compared to the control group. However, the MDA levels in the BLM+Theo group were also significantly higher than in the control group (p&lt; 0.01). Similarly, the GSH levels were significantly higher in the BLM+Theo group than in the BLM group (p&lt; 0.05). The results indicated that Theo reduced the BLM-induced activation of nuclear factor-kappaB (NF-κB) and decreased interleukin-6 (IL-6) levels, together with significant amelioration of the immunohistochemical and histopathological architecture in the lung tissues. It was concluded that the administration of Theo had a positive effect on the GSH level, and activation of NF-κB and IL-6 expression, which were significant proinflammatory markers in the BLM-treated rats.
DOI: 10.6084/m9.figshare.21744152
title: Tangency and multiple factors of violence against lecturer: nuances of the experience in pedagogical practices in health education

description: ABSTRACT Objective: to identify factors that lead the teacher to experience violence in their pedagogical practice in health education. Method: research with a qualitative approach, based on the Grounded Theory, conducted with 11 professors of the nursing course of a public university in the central region of Brazil in 2020 and 2021. Online semi-structured interviews were analyzed partially in the light of the Constructivist Grounded Theory. Results: factors that lead lecturer to experience violence are characterized by institutional culture, gender, professor’s perception of violence, and the triggers that drive students to commit violence. Social status and inequalities lead to positions of domination and, consequently, create a fertile ground for violence. Final Considerations: analyzing violence under Bourdieu’s theory, it is clear that student violence towards lecturer and the reports contained in this study deserve pedagogical reflection. However, it is necessary to include these discussions as a background in teaching environments.
DOI: 10.6084/m9.figshare.21744152.v1
title: Tangency and multiple factors of violence against lecturer: nuances of the experience in pedagogical practices in health education

description: ABSTRACT Objective: to identify factors that lead the teacher to experience violence in their pedagogical practice in health education. Method: research with a qualitative approach, based on the Grounded Theory, conducted with 11 professors of the nursing course of a public university in the central region of Brazil in 2020 and 2021. Online semi-structured interviews were analyzed partially in the light of the Constructivist Grounded Theory. Results: factors that lead lecturer to experience violence are characterized by institutional culture, gender, professor’s perception of violence, and the triggers that drive students to commit violence. Social status and inequalities lead to positions of domination and, consequently, create a fertile ground for violence. Final Considerations: analyzing violence under Bourdieu’s theory, it is clear that student violence towards lecturer and the reports contained in this study deserve pedagogical reflection. However, it is necessary to include these discussions as a background in teaching environments.