cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.13951769
title: Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking

description: This upload contains raw data and simulation software underlying the results presented in a manuscript submitted for peer-review, with the title:

 

Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking

 

Authored by:

C Venugopal Srambickal1,*, H Esmaeeli1,*, J Piguet1, L Reinkensmeier2, R Siegmund2, M Bates2, A Egner2, J Widengren1,**

1 Experimental Biomolecular Physics, Bio-Opto-Nano Unit, Department of Applied Physics, Royal Institute of Technology, SE-10691 Stockholm, Sweden

2 Department of Optical Nanoscopy, Institute for Nanophotonics, D-37077 Göttingen, Germany

* Contributed equally

** Corresponding author: jwideng@kth.se

 

ABSTRACT

MINimal photon FLUXes (MINFLUX) offers super-resolution microscopy (SRM) with nanometer localization precision, with lower fluorophore brightness and photostability requirements than for other SRM techniques. Nonetheless, low localization probabilities have been reported in several MINFLUX studies, and a broader use of less bright and photostable fluorophores, including near-infrared (NIR) fluorophores has been difficult to realize. In this work, we identified fluorophore blinking as a main cause of erroneous (and dismissed) fluorophore localizations in MINFLUX imaging and devised strategies to overcome these effects. We systematically studied the blinking/switching properties of cyanine fluorophores emitting in the far-red or NIR range, over typical time scales (µs-10ms), sample and excitation conditions used in MINFLUX imaging. Subsequent simulations of representative MINFLUX localization procedures showed that trans-cis isomerization, and in particular photo-reduction of the fluorophores, can generate significant localization errors. These localization errors, however, could be suppressed by balanced redox buffers and repetitive excitation beam scans. Implementing these strategies, and replacing the slower, intrinsic switching of the fluorophores needed for the localization by transient binding of fluorophore-labelled DNA strands to complementary DNA strands attached to the targets (DNA-PAINT), we could for the first time demonstrate NIR-MINFLUX imaging with nanometer localization precision. This work presents an overall strategy, where fluorophore blinking characterization and subsequent simulations make it possible to design optimal sample and excitation conditions, opening for NIR-MINFLUX imaging, as well as for a broader use of fluorophores in MINFLUX and related SRM studies.

Acknowledgements:

This study was supported by the European Union's Horizon 2020 research and innovation program under grant agreement 101017180 (NanoVIB).

 

Files with raw data on which the manuscript is based are grouped into folders according to the figures/tables in the manuscript where the extracted results are presented. Additionally, software developed and used for the simulations are arranged into a separate folder.
DOI: 10.15468/dl.wb6639
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "TaxonKey is one of (Lapsanastrum uncinatum (Stebbins) Pak & K.Bremer)"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028411-241107131044228/datasets/export for details.
DOI: 10.5281/zenodo.10996742
title: Schiff Base Crosslinked Hyaluronic Acid Hydrogels with Tunable and Cell Instructive Time-Dependent Mechanical Properties

description: This folder contains raw data for the paper titled "Schiff Base Crosslinked Hyaluronic Acid Hydrogels with Tunable and Cell Instructive Time-Dependent Mechanical Properties" authored by Taha Behroozi Kohlan, Yanru Wen, Carina Milena Mini, and Anna Finne-Wistrand, published in Carbohydrate Polymers journal. The raw data presented here contains NMR, FTIR, SEC, swelling and stability, and rheology data used to create figures.

Abstract:

The dynamic interplay between cells and their native extracellular matrix (ECM) influences cellular behavior, imposing a challenge in biomaterial design. Dynamic covalent hydrogels are viscoelastic and show self-healing ability, making them a potential scaffold for recapitulating native ECM properties. We aimed to implement kinetically and thermodynamically distinct crosslinkers to prepare self-healing dynamic hydrogels to explore the arising properties and their effects on cellular behavior. To do so, aldehyde-substituted hyaluronic acid (HA) was synthesized to generate imine, hydrazone, and oxime crosslinked dynamic covalent hydrogels. Differences in equilibrium constants of these bonds yielded distinct properties including stiffness, stress relaxation, and self-healing ability. The effects of degree of substitution (DS), polymer concentration, crosslinker to aldehyde ratio, and crosslinker functionality on hydrogel properties were evaluated. The self-healing ability of hydrogels was investigated on samples of the same and different crosslinkers and DS to obtain hydrogels with gradient properties. Subsequently, human dermal fibroblasts were cultured in 2D and 3D to assess the cellular response considering the dynamic properties of the hydrogels. Moreover, assessing cell spreading and morphology on hydrogels having similar modulus but different stress relaxation rates showed the effects of matrix viscoelasticity with higher cell spreading in slower relaxing hydrogels.
DOI: 10.5281/zenodo.10996743
title: Schiff Base Crosslinked Hyaluronic Acid Hydrogels with Tunable and Cell Instructive Time-Dependent Mechanical Properties

description: This folder contains raw data for the paper titled "Schiff Base Crosslinked Hyaluronic Acid Hydrogels with Tunable and Cell Instructive Time-Dependent Mechanical Properties" authored by Taha Behroozi Kohlan, Yanru Wen, Carina Milena Mini, and Anna Finne-Wistrand, published in Carbohydrate Polymers journal. The raw data presented here contains NMR, FTIR, SEC, swelling and stability, and rheology data used to create figures.

Abstract:

The dynamic interplay between cells and their native extracellular matrix (ECM) influences cellular behavior, imposing a challenge in biomaterial design. Dynamic covalent hydrogels are viscoelastic and show self-healing ability, making them a potential scaffold for recapitulating native ECM properties. We aimed to implement kinetically and thermodynamically distinct crosslinkers to prepare self-healing dynamic hydrogels to explore the arising properties and their effects on cellular behavior. To do so, aldehyde-substituted hyaluronic acid (HA) was synthesized to generate imine, hydrazone, and oxime crosslinked dynamic covalent hydrogels. Differences in equilibrium constants of these bonds yielded distinct properties including stiffness, stress relaxation, and self-healing ability. The effects of degree of substitution (DS), polymer concentration, crosslinker to aldehyde ratio, and crosslinker functionality on hydrogel properties were evaluated. The self-healing ability of hydrogels was investigated on samples of the same and different crosslinkers and DS to obtain hydrogels with gradient properties. Subsequently, human dermal fibroblasts were cultured in 2D and 3D to assess the cellular response considering the dynamic properties of the hydrogels. Moreover, assessing cell spreading and morphology on hydrogels having similar modulus but different stress relaxation rates showed the effects of matrix viscoelasticity with higher cell spreading in slower relaxing hydrogels.
DOI: 10.15468/dl.7y6pd7
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Hieracium pilosum Schleich., 1815)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 2000"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028412-241107131044228/datasets/export for details.
DOI: 10.15468/dl.nsppk6
title: Occurrence Download

description: A dataset containing 2148 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is POL.9.5.1_1",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 2148 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028413-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9wm2k9
title: Occurrence Download

description: A dataset containing 6450 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Oxyura jamaicensis (J.F.Gmelin, 1789)",
 "Country is France",
 "Year is greater than or equal to 1500"
 ]
}

 The dataset includes 6450 records from 81 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028414-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.g3k63f
title: Occurrence Download

description: A dataset containing 2008 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is United States of America",
 "DatasetKey is iNaturalist Research-grade Observations",
 "GadmGid is USA.44_1",
 "TaxonKey is Bombus sonorus Say, 1837"
 ]
}

 The dataset includes 2008 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0199278-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gmfudu
title: Occurrence Download

description: A dataset containing 11984 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is United States of America",
 "DatasetKey is iNaturalist Research-grade Observations",
 "GadmGid is USA.44_1",
 "TaxonKey is Bombus pensylvanicus (De Geer, 1773)"
 ]
}

 The dataset includes 11984 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0199285-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.sng27x
title: Occurrence Download

description: A dataset containing 9 species occurrences available in GBIF matching the query:
{
 "and" : [
 "CatalogNumber is one of (136987135, 140707549, 184026925, 112189588, 22625025, 132718729, 83936705, 79218064, 72197699)",
 "PublishingOrg is 28eb1a3f-1c15-4a95-931a-4af90ecb574d"
 ]
}

 The dataset includes 9 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046787-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fwppxw
title: Occurrence Download

description: A dataset containing 7 species occurrences available in GBIF matching the query:
{
 "and" : [
 "CatalogNumber is one of (229991368, 224474910, 18656953, 3811835, 223820554, 165365765, 221162238)",
 "PublishingOrg is 28eb1a3f-1c15-4a95-931a-4af90ecb574d"
 ]
}

 The dataset includes 7 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046779-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.u8u39w
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "CatalogNumber is 140995169",
 "PublishingOrg is 28eb1a3f-1c15-4a95-931a-4af90ecb574d"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0033933-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.254q7x
title: Occurrence Download

description: A dataset containing 7151 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Hieracium villosum Jacq.)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 2000"
 ]
}

 The dataset includes 7151 records from 74 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028416-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gn8p3e
title: Occurrence Download

description: A dataset containing 707 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Polistes marginalis (Fabricius, 1775)"
 ]
}

 The dataset includes 707 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028417-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.27890052
title: <b>Prefrontal Cortex Contribution in Transitive Inference</b>

description: Prefrontal Cortex Contribution in Transitive Inference Task Through the Interplay of Beta and Gamma oscillations
DOI: 10.6084/m9.figshare.27890052.v1
title: <b>Prefrontal Cortex Contribution in Transitive Inference</b>

description: Prefrontal Cortex Contribution in Transitive Inference Task Through the Interplay of Beta and Gamma oscillations
DOI: 10.15468/dl.vuetj9
title: Occurrence Download

description: A dataset containing 44502 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Homogyne alpina (L.) Cass.)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 2000"
 ]
}

 The dataset includes 44502 records from 198 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028418-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3886/e119803
title: National Neighborhood Data Archive (NaNDA): Parks by ZIP Code Tabulation Area, United States, 2018

description: This dataset describes the number and area of parks in each ZIP code tabulation area (ZCTA) in the United States. Measures include the total number of parks, park area, and proportion of park area within each ZCTA.
description: A ZIP code to ZCTA crosswalk must be used to combine this dataset with ZIP code geocoded data must use a ZIP code to ZCTA crosswalk. Such a crosswalk is available on the UDS Mapper website at https://www.udsmapper.org/zcta-crosswalk.cfm. Sample code for merging the UDS Mapper crosswalk with NaNDA datasets is available on the NaNDA repository at https://www.openicpsr.org/openicpsr/project/120088/.
description: ZCTAs in the United States, including Alaska and Hawaii. Smallest Geographic Unit: ZIP code tabulation area
DOI: 10.3886/e119803v1-159461
title: National Neighborhood Data Archive (NaNDA): Parks by ZIP Code Tabulation Area, United States, 2018

description: This dataset describes
the number and area of parks in each ZIP code tabulation area (ZCTA) in the United States.
Measures include the total number of parks, park area, and proportion of park
area within each ZCTA.<br><br>A curated version of this data is available through ICPSR at https://www.icpsr.umich.edu/web/ICPSR/studies/38586/versions/V1<br>
description: ZCTAs in the United States, including Alaska and Hawaii. Smallest Geographic Unit: ZIP code tabulation area
description: A ZIP code to ZCTA crosswalk must be used to combine this dataset with ZIP code geocoded data must use a ZIP code to ZCTA crosswalk. Such a crosswalk is
available on the UDS Mapper website at https://udsmapper.org/zip-code-to-zcta-crosswalk/. Sample code for merging the UDS Mapper crosswalk with
NaNDA datasets is available on the NaNDA repository at https://www.openicpsr.org/openicpsr/project/120088/.
DOI: 10.15468/dl.m855be
title: Occurrence Download

description: A dataset containing 5 records in GBIF matching the query:
SELECT specieskey, countrycode AS countries FROM occurrence WHERE specieskey IS NOT NULL AND occurrencestatus = 'PRESENT' GROUP BY specieskey, countrycode LIMIT 5

 The dataset's 5 records were derived from 63382 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028365-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25582/data.2024-02.3034793/2548780
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034794/2548781
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034795/2548782
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034796/2548783
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034797/2548784
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034798/2548785
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034799/2548786
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034800/2548787
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034801/2548788
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034802/2548789
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034803/2548790
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034804/2548791
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034805/2548792
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034806/2548793
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034807/2548794
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-02.3034808/2548795
title: Data for EMSL Project 60579 from February 2024
DOI: 10.25582/data.2024-04.3083409/2548796
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083410/2548797
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083412/2548798
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083413/2548799
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083414/2548800
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083415/2548801
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083417/2548802
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083419/2548803
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083420/2548804
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083421/2548805
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083424/2548806
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083425/2548807
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083426/2548808
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083429/2548809
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083430/2548810
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083436/2548811
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083438/2548812
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083442/2548813
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083443/2548814
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083445/2548815
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083446/2548816
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083447/2548817
title: Data for EMSL Project 51287 from April 2024
DOI: 10.25582/data.2024-04.3083448/2548818
title: Data for EMSL Project 51287 from April 2024
DOI: 10.15468/dl.rwkyb5
title: Occurrence Download

description: A dataset containing 573163 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Amaranthus blitum L., Amaranthus crassipes Schltdl., Amaranthus crispus (Lesp. & Thévenau) N.Terracc., Amaranthus muricatus (Gillies ex Moq.) Hieron., Amaranthus thunbergii Moq., Amaranthus tricolor L., Amaryllis belladonna L., Amberboa moschata (L.) DC., Momordica balsamina L., Momordica charantia L., Pontederia vaginalis Burm.f., Monstera deliciosa Liebm., Montanoa hibiscifolia (Benth.) K.Koch, Moorochloa eruciformis (Sm.) Veldkamp, Moraea collina Thunb., Moraea polystachya (Thunb.) Ker Gawl., Myrica cerifera Bigelow, Myrica faya (Dryand.) Aiton, Myrica rubra A.Chev., Morinda citrifolia L., Moringa oleifera Lam., Broussonetia papyrifera (L.) L'Hér. ex Vent., Morus nigra L., Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim., Mucuna pruriens (L.) DC., Muehlenbeckia axillaris (Hook.fil.) Walp., Muehlenbeckia complexa (A.Cunn.) Meisn., Muhlenbergia paniculata (Nutt.) P.M.Peterson, Mummenhoffia alliacea (L.) Esmailbegi & Al-Shehbaz, Muntingia calabura L., Murdannia keisak (Hassk.) Hand.-Mazz., Murdannia nudiflora (L.) Brenan, Murdannia spirata (L.) G.Brückn., Murraya paniculata (L.) Jacq., Musa acuminata Colla, Musa troglodytarum L., Musa velutina H.Wendl. & Drude, Muscari armeniacum H.J.Veitch, Myagrum perfoliatum L., Myoporum laetum G.Forst., Myosotis azorica H.C.Watson, Myosotis latifolia Poir., Myosotis sylvatica Ehrh. ex Hoffm., Stellaria aquatica (L.) Scop., Myrciaria floribunda (West ex Willd.) O.Berg, Myriophyllum aquaticum (Vell.) Verdc., Myrrhis odorata (L.) Scop., Myrtus communis L., Najas graminea Delile, Najas wrightiana A.Braun)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 573163 records from 2764 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009488-250402121839773/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9fqmhh
title: Occurrence Download

description: A dataset containing 27804 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Venezuela (Bolivarian Republic of)",
 "HasCoordinate is true",
 "TaxonKey is Mammalia",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 27804 records from 38 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017208-190415153152247/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bclj3d
title: Occurrence Download

description: A dataset containing 390244 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Linaria Mill."
 ]
}

 The dataset includes 390244 records from 916 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028254-200221144449610/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.27leyb
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-180 -90,-180 30,0 30,0 -90,-180 -90))",
 "HasCoordinate is true",
 "TaxonKey is Dracula astuta (Rchb.f.) Luer",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 5 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0015048-180131172636756/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.