DOI: 10.5061/dryad.76hdr7t8r
title: Data from: Excitation-contraction coupling, cardiomyocyte electrophysiology, and transcriptome profiles in two HFpEF murine models: Etiology and sex-dependent differences

description: This dataset contains the source data supporting the article entitled
 Excitation-contraction coupling, cardiomyocyte electrophysiology, and
 transcriptome profiles in two HFpEF murine models: Etiology and
 sex-dependent differences. The dataset supports key findings in recent
 two-hit translational animal models of heart failure with preserved
 ejection fraction (HFpEF), including the hypertensive,
 nitrosative-stressed mice fed with high-fat diet and L-NAME (HFD+L-NAME)
 and the obese-diabetic leptin receptor-deficient db/db mice with excess
 aldosterone (db/db+Aldo), which may phenocopy select subgroups of HFpEF.
 Morphometry, echocardiography, electrophysiology (action potentials and
 ionic currents), fluorescent Ca imaging, and RNA sequencing data are
 included in separate Excel sheets with individual cell data and group
 statistics. The dataset is structured to follow the sequence of data
 presented in the Figures in the article and to ease the navigation between
 the uploaded data files.
description: # Data from: Excitation-contraction coupling, cardiomyocyte
 electrophysiology, and transcriptome profiles in two HFpEF murine models:
 Etiology and sex-dependent differences Dataset DOI:
 [10.5061/dryad.76hdr7t8r](https://doi.org/10.5061/dryad.76hdr7t8r) ##
 Description of the data and file structure This dataset contains
 morphometry, echocardiography, electrophysiology (action potentials and
 ionic currents), fluorescent Ca imaging, and RNA sequencing data in
 separate Excel sheets with individual cell data and group statistics.
 Files are compressed to keep the folder structure and to ease navigation
 between the uploaded documents. For a full description of the materials
 and methods, including experimental procedures and instrumentation, please
 refer to the article on the American Journal of Physiology - Heart and
 Circulatory Physiology website. #### File: Action_potential.zip
 Description: This compressed file contains two Excel files with action
 potential (AP) data in 3 groups of experimental animals, including (1)
 control sham, (2) leptin receptor-deficient db/db mice with chronic
 aldosterone infusion (db/db+Aldo), and (3) high-fat diet combined with
 nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME). Male and
 female data are reported separately in each group.
 "Fig3_data_AP-parameters.xlsx" provides ventricular
 cardiomyocyte AP data at 1 Hz steady-state pacing frequency. Sheet
 "Panel A" provides typical AP waveforms data (time in
 milliseconds and membrane voltage in millivolts). Sheet "Panel
 B" provides AP repolarization parameters, including AP duration at
 20, 50, and 90% of repolarization (APD20, APD50, and APD90, respectively)
 in milliseconds. Sheet "Panel C" provides additional AP
 parameters, including resting membrane potential (RMP, in millivolts),
 maximal rate of AP upstroke velocity (dV/dtmax, in volts per second), and
 maximal rate of AP phase 3 repolarization (-dV/dtmax, in volts per
 second). "Fig4_data_Frequency-STV-Alternans.xlsx" provides
 ventricular cardiomyocyte AP data at various pacing rates from 1 Hz to 10
 Hz. Sheet "Panel A" provides data about the frequency dependence
 of AP duration at 90% of repolarization (APD90, in milliseconds). Sheet
 "Panel B" provides typical AP waveforms data at a 10 Hz pacing
 rate (time in milliseconds and membrane voltage in millivolts). Sheet
 "Panel C" provides fifty consecutive APD90 data at a 1 Hz pacing
 rate (time in seconds, APD90 in milliseconds). Sheet "Panel D"
 provides the short-term variability of APD90 at 1 Hz pacing (STV, in
 milliseconds), the magnitude of APD90 alternans (difference between long
 and short beats) at 10 Hz pacing (in milliseconds). #### File:
 Ca_imaging.zip Description: This compressed file contains two Excel files
 with fluorescent intracellular Ca2+ imaging (using Fluo-4) data in 3
 groups of experimental animals, including (1) control sham, (2) leptin
 receptor-deficient db/db mice with chronic aldosterone infusion
 (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase
 inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported
 separately in each group. "Fig10_data_CaT.xlsx" provides
 ventricular cardiomyocyte intracellular Ca2+ transient (CaT) data. Sheet
 "Panel B" provides typical caffeine-induced Ca2+ transient (Caff
 T) data (time in milliseconds and fluorescence expressed as background
 corrected F/F0, where F0 is the non-paced baseline fluorescence). Sheet
 "Panel C" provides CaT parameters data at 1 Hz steady-state
 pacing, including CaT amplitude (difference between baseline and peak
 fluorescence, normalized to non-paced F0), CaT tau decay (in
 milliseconds), and sarcoplasmic reticulum (SR) Ca2+ load assessed by rapid
 caffeine application (difference between baseline and peak fluorescence,
 normalized to non-paced F0). Sheet "Panel D" provides CaT
 parameters at 2 Hz steady-state pacing, including CaT amplitude
 (difference between baseline and peak fluorescence, normalized to
 non-paced F0), CaT tau decay (in milliseconds), and Caff T decay slope
 (change in fluorescence per second).
 "Fig11_data_Ca-sparks-and-waves.xlsx" provides diastolic Ca2+
 spark data in ventricular cardiomyocytes. Sheet "Panel B"
 provides Ca2+ spark frequency (number of Ca2+ sparks per 100 micrometers
 per second) and Ca2+ wave frequency (number of waves per minute). ####
 File: Echocardiography.zip Description: This compressed file contains one
 Excel file with transthoracic echocardiography data in 3 groups of
 experimental animals, including (1) control sham, (2) leptin
 receptor-deficient db/db mice with chronic aldosterone infusion
 (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase
 inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported
 separately in each group. "Fig2_data_Echocardiography.xlsx"
 provides key echocardiographic parameters to characterize systolic and
 diastolic heart function. Sheet "Panel B" provides systolic
 heart function parameters: ejection fraction (EF, %), left ventricular
 (LV) mass (in milligrams), and left ventricular remodeling index (LVRI,
 milligram/millimeter) data. Sheet "Panel C" provides diastolic
 heart function parameters: early to late filling velocity ratio (ratio
 between mitral E wave and A wave, E/A), ratio between mitral E wave and e’
 wave (E/e'), and left atrial (LA) area (in square millimeters) data.
 #### File: Ionic_currents.zip Description: This compressed file contains
 five Excel files with cellular electrophysiology data in 3 groups of
 experimental animals, including (1) control sham, (2) leptin
 receptor-deficient db/db mice with chronic aldosterone infusion
 (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase
 inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported
 separately in each group. "Fig5_data_Capacitance.xlsx" provides
 cell membrane capacitance (in picofarads) "Fig6_data_IK1.xlsx"
 provides data on the inward rectifier potassium current (IK1). Sheet
 "Panel A" provides typical IK1 traces (time in milliseconds and
 IK1 current in picoampere per picofarad). Sheet "Panel B"
 provides the net magnitude of IK1 at -140 millivolts and at -40 millivolts
 (in picoamperes). Sheet "Panel C" provides the density of IK1
 (IK1 magnitude normalized to cell capacitance) at -140 millivolts and at
 -40 millivolts (in picoampere per picofarad).
 "Fig7_data_IKv.xlsx" provides data on the voltage-gated
 potassium currents (IKv). Sheet "Panel A" provides typical IKv
 traces (time in milliseconds and IKv current in picoampere per picofarad).
 Sheet "Panel B" provides the net magnitude of the transient
 outward K+ current (Ito), the slowly inactivating potassium current (IK,
 slow), and the sustained potassium current (Isus) at 60 millivolts (in
 picoamperes). These potassium currents were separated by biexponential
 fitting to I~Kv~ traces. Sheet "Panel C" provides the density of
 Ito, IK, slow, and Isus (magnitude normalized to cell capacitance) at 60
 millivolts (in picoampere per picofarad). "Fig8_data_ICa,L.xlsx"
 provides data on the L-type calcium current (ICa,L). Sheet "Panel
 A" provides typical ICa,L traces (time in milliseconds and ICa,L
 current in picoampere per picofarad). Sheet "Panel B" provides
 the voltage-dependence of ICa,L (current-voltage relationship, I-V).
 Voltage steps are expressed in millivolts, and currents are expressed in
 picoampere per picofarad. Sheet "Panel C" provides the net
 magnitude (in picoamperes) and the density (magnitude normalized to cell
 capacitance, in picoampere per picofarad) of ICa,L.
 "Fig9_data_INa,L.xslx" provides data on the late sodium current
 (INa,L). Sheet "Panel A" provides typical INa,L traces (time in
 milliseconds and INa,L current in picoampere per picofarad). Sheet
 "Panel B" provides the net magnitude (in picoamperes) and the
 density (magnitude normalized to cell capacitance, in picoampere per
 picofarad) of INa,L. #### File: Morphometry.zip Description: This
 compressed file contains one Excel file with morphometric data in 3 groups
 of experimental animals, including (1) control sham, (2) leptin
 receptor-deficient db/db mice with chronic aldosterone infusion
 (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase
 inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported
 separately in each group. "Fig1_Data_Morphometry.xlsx" provides
 key morphometric data. Sheet "Panel B" provides body weight data
 (in grams). Sheet "Panel C" provides blood glucose data (in
 milligrams/deciliter). Sheet "Panel D" provides heart weight to
 tibial length ratio (HW/Tl, in gram/centimeter). Sheet "Panel E"
 provides wet to wet-to-dry lung weight ratio. Sheet "Panel F"
 provides liver weight (in grams). #### File: RNA-sequencing.zip
 Description: This compressed file contains 5 Excel files with
 RNA-sequencing data. "Data Set
 S1_dbdb+Aldo_vs_Vehicle_Control_DEGenes.xslx" provides differentially
 expressed genes (DEGs) in leptin receptor-deficient db/db mice with
 chronic aldosterone infusion (db/db+Aldo) murine hearts versus wild-type
 sham controls. "Data Set
 S2_HFD+L-NAME_vs_Chow_Control_DEGenes.xlsx" provides differentially
 expressed genes (DEGs) in high-fat diet combined with nitric oxide
 synthase inhibitor L-NAME treatment (HFD+L-NAME) murine hearts versus
 normal chow controls. "Data Set S3_Model Comparison Genes
 Labelled.xlsx" provides differentially expressed genes (DEGs)
 comparison between two HFpEF murine models: leptin receptor-deficient
 db/db mice with chronic aldosterone infusion (DB), and high-fat diet
 combined with nitric oxide synthase inhibitor L-NAME treatment (HFD).
 "Data Set S4_dbdb+Aldo_female_vs_male_DEGenes.xlsx"
 provides differentially expressed genes (DEGs) in female versus male
 hearts in the leptin receptor-deficient db/db mice with chronic
 aldosterone infusion (db/db+Aldo) model. "Data Set
 S5_HFD+L-NAME_female_vs_male_DEGenes.xlsx" provides differentially
 expressed genes (DEGs) in female versus male hearts in the high-fat diet
 combined with nitric oxide synthase inhibitor L-NAME treatment
 (HFD+L-NAME) model. ## Code/software Microsoft Excel can be used to view
 the data files.