DOI: 10.5061/dryad.5p7647r
title: Data from: Environmental concentrations of antibiotics may diminish Wolbachia infections in Aedes aegypti (Diptera: Culicidae)

description: Wolbachia-infected Aedes aegypti (L.) mosquitoes for control of dengue
 transmission are being released experimentally in tropical regions of
 Australia, south-east Asia, and South America. To become established, the
 Wolbachia Hertig (Rickettsiales: Rickettsiaceae) strains used must induce
 expression of cytoplasmic incompatibility (CI) in matings between infected
 males and uninfected females so that infected females have a reproductive
 advantage, which will drive the infection through field populations.
 Wolbachia is a Rickettsia-like alphaproteobacterium which can be affected
 by tetracycline antibiotics. We investigated whether exposure of
 Wolbachia-infected mosquitoes to chlortetracycline at environmentally
 relevant levels during their aquatic development resulted in loss or
 reduction of infection in three strains, wAlbB, wMel, and wMelPop.
 Wolbachia density was reduced for all three strains at the tested
 chlortetracycline concentrations of 5 and 50 µg/liter. Two of the strains,
 wMel and wMelPop, showed a breakdown in CI. The wAlbB strain maintained CI
 and may be useful at breeding sites where tetracycline contamination has
 occurred. This may include drier regions where Ae. aegypti can utilize
 subterranean water sources and septic tanks as breeding sites.
description: Antibiotic trial
 development data for Aedes aegyptiCount data from Aedes
 aegypti antibiotic trial - cumulative pupae counts, number of emerged
 adults from each treatment.20190207 Antibiotic trial
 development data.xlsxAntibiotic trial fitness
 data for Aedes aegyptiEgg counts and
 percentage hatch from antibiotic trial on Aedes
 aegypti.20190207 Antibiotic trial
 fitness data.xlsxWolbachia screening of
 TSV strain females in antibiotic trial of Aedes aegyptiWolbachia-screening data
 for females Aedes aegypti from an uninfected line (control). Colony
 derived from eggs collected in Townsville, Queensland, Australia. Data
 collected on the Roche LightCycler 480 with an HRM
 assay.20190206 TSV ABT
 Analysis.xlsxWolbachia screen of
 Aedes aegypti wMel females from antibiotic trialWolbachia-screening data
 from female Aedes aegypti infected with wMel (Walker et al. 2011) strain.
 High Resolution Melt Assay on Roche LightCycler480.20190206
 wMel_ABT_HRM_females.xlsxWolbachia screen of
 female Aedes aegypti wMelPop-CLA strain in antibiotic
 trialWolbachia-screening data
 from female Aedes aegypti infected with wMelPop-CLA (McMeniman et al.
 2009) strain. High Resolution Melt Assay on Roche
 LightCycler480.20190206_wMelPop_ABT_HRM_females.xlsxWolbachia screen of Aedes aegypti wAlbB females in antibiotic trialWolbachia-screening data from female Aedes aegypti infected with wAlbB (Xi et al. 2005) strain in antibiotic trial. High Resolution Melt Assay on Roche LightCycler480.20190206_wAlbB_ABT_HRM_females.xlsxAntibiotic trial male Aedes aegypti data - ddPCR for WolbachiaMale Aedes aegypti from four lines in antibiotic trial. Wolbachia-screening data obtained via QX100™ Droplet Digital™ PCR (ddPCR™) system (Bio-Rad Laboratories Pty., Ltd. Hercules, CA USA) with a hydrolysis probe assay (PrimePCR™, Bio-Rad Laboratories, Inc. Cat. No. 10031261).20180816 Antibiotic ddPCR male data.xlsx