cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ccsvx-vn742
title: Agro_AD1484

description: Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid).Cb resistance. Grown on induction plate pH5.8
description: <strong>Tilt Series Date:</strong> 2015-10-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid). Cells were scraped off the induction plate (particularly from the immediate surface of the plate: this is to ensure that if something is happening on the real surface of the plate, we do not miss it), dissolved in induction media, spun for 15 min in a table top centrifuge, supernatant thrown away, cells redissolved in PBS. Add gold to this: 1ul of gold to 4 ul of cells and freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-70</p>20151026_AD1484_30001_part81_10.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-70/3dimage_70940/20151026_AD1484_30001_part81_10.rec"
        > <i class="download icon"></i> Download </a></p>

        20151026_AD1484_30001.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-70/rawdata/20151026_AD1484_30001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3eree-qdq42
title: dpil

description: This is a mutant in which pil system, a type 4 pili in Shewanella has been knocked down. This was done to test whether the pili I see in wt are msh or pilsystem. Turns out, what I see in wt is msh because in the msh knockdown they are absent and pil knockdown they are present. Pil M-Q has been knocked down. This is the complete biosynthetic machinery.
description: <strong>Tilt Series Date:</strong> 2015-07-09</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in serum bottles</p>
<strong>Sample Preparation:</strong> blotting conditions 6 - blot force,3 blot time,1s-drain time</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-09-8</p>atlas4_dpil0008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-09-8/rawdata/atlas4_dpil0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas4_dpil0008_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-09-8/3dimage_58960/atlas4_dpil0008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hx7y5-2rm13
title: stable HAMP mutant

description: UU2612 plus stable HAMP
description: <strong>Tilt Series Date:</strong> 2015-04-15</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sandy Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB, Gent Amp 50µl 10 mM IPTGPenicillin lysed</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-8</p>stableHAMP1set0003.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-8/rawdata/stableHAMP1set0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        stableHAMP1set0003_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-8/3dimage_54076/stableHAMP1set0003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jb6v1-z4e64
title: Cos7 cells

description: Looking for midbodies and tunneling nanotubes
description: <strong>Tilt Series Date:</strong> 2015-02-27</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Cos7</p>
<strong>Strain:</strong> Cos7 cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Sample Preparation:</strong> Cos7 cells + Thymidine 2mM synchronization.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-02-27-2</p>cos7pagfp_atlas10002.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-02-27-2/rawdata/cos7pagfp_atlas10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        cos7pagfp_atlas10002_part121_57.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-02-27-2/3dimage_52343/cos7pagfp_atlas10002_part121_57.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25919/5mpx-cn87
title: Parkes observations for project P737 semester 2023APRS_02

description: The aim of this proposal is to carry out tests of the new backend instruments which cannot be carried out during standard maintenance periods. The scheduled time is used to measure beam shapes, determine polarisation calibration solutions and commission the National Facility observing modes for the new wide-band receiver.
DOI: 10.22002/qn641-0t762
title: Myxococcus xanthus SA6888

description: Strain SA6888 (minor pilin cluster 1+2 deletion, Δ mxan1021-1017;  Δmxan0364-0359).Grid box YWC224
description: <strong>Tilt Series Date:</strong> 2016-09-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-15-53</p>20160915_YWC224_10053.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-15-53/rawdata/20160915_YWC224_10053.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160915_YWC224_10053_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-15-53/3dimage_79203/20160915_YWC224_10053_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7nz49-qcy90
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-10-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-13</p>Cb_vero_201710190011.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-13/rawdata/Cb_vero_201710190011.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_201710190011_part61_1.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-13/3dimage_89462/Cb_vero_201710190011_part61_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nq2sp-9g538
title: Pombe Cryosections

description: These cells were high pressure frozen after the majority of the rings were around mid-division.
description: <strong>Tilt Series Date:</strong> 2015-05-30</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Manual/Raptor</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-30-32</p>053015_cryosection1_square6_tomo0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-30-32/rawdata/053015_cryosection1_square6_tomo0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        053015_cryosection1_square6_tomo0004.rec,  Reconstruction (Pixel Size 2.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-05-30-32/3dimage_57701/053015_cryosection1_square6_tomo0004.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e3mmp-kfb55
title: P41+P41

description: Raw data files of P41+P41
description: <strong>Tilt Series Date:</strong> 2016-03-15</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Mycoplasma pneumoniae</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Edward Sheppard from Duncan Krause's lab prepared the cell culture</p>
  scy2016-03-15-20_slicer16783.jpg: attachment organelle? scy2016-03-15-20_slicer16784.jpg: 2 attachment organelles scy2016-03-15-20_slicer16785.jpg: attachment organelle
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-03-15-20</p>atlas10019.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-03-15-20/rawdata/atlas10019.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10019_full.rec,  Reconstruction (Pixel Size 1.56 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-03-15-20/3dimage_73616/atlas10019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25919/vbm9-0m12
title: Parkes observations for project P737 semester 2023APRS_02

description: The aim of this proposal is to carry out tests of the new backend instruments which cannot be carried out during standard maintenance periods. The scheduled time is used to measure beam shapes, determine polarisation calibration solutions and commission the National Facility observing modes for the new wide-band receiver.
DOI: 10.25919/kmek-6k98
title: Parkes observations for project P737 semester 2023APRS_02

description: The aim of this proposal is to carry out tests of the new backend instruments which cannot be carried out during standard maintenance periods. The scheduled time is used to measure beam shapes, determine polarisation calibration solutions and commission the National Facility observing modes for the new wide-band receiver.
DOI: 10.22002/k4be6-va953
title: AD348_delVirB_Agro

description: Mutant Agro cells, delVirB (whole of the VirB system deleted). Grown on induction plate pH5.8 in exactly similar way as the AD1484 strain.
description: <strong>Tilt Series Date:</strong> 2016-01-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow at 19C for 4 days on induction plate. Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD348_delVirB) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-06-23</p>AD348_delVirB0023.rec,  Reconstruction (Pixel Size 19.2 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-06-23/3dimage_72213/AD348_delVirB0023.rec"
        > <i class="download icon"></i> Download </a></p>

        AD348_delVirB0023.mrc,  Tilt Series (Pixel Size 4.8 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-06-23/rawdata/AD348_delVirB0023.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s7pdb-4zk48
title: Xanthomonas vs minicells

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Ec-mini cells
description: <strong>Tilt Series Date:</strong> 2017-04-27</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-53.0°, 53.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the strains, data collected by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Ec-mini cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 sec</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-48</p>Xac-mini-20170427x30001.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-48/rawdata/Xac-mini-20170427x30001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac-mini-20170427x30001_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-48/3dimage_109254/Xac-mini-20170427x30001_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        Xac-mini-20170427x30001.ali,  , 484.6 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-48/file_26259/Xac-mini-20170427x30001.ali"
        > <i class="download icon"></i> Download </a></p>

        Xac-mini-20170427x30001.tlt,  , 544.0 B  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-48/file_26260/Xac-mini-20170427x30001.tlt"
        > <i class="download icon"></i> Download </a></p>

        Xac-mini-20170427x30001_fid.tlt,  , 544.0 B  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-48/file_26261/Xac-mini-20170427x30001_fid.tlt"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.22262196
title: Chronic jetlag accelerates pancreatic neoplasia in conditional <i>Kras</i>-mutant mice

description: Misalignment of the circadian clock compared to environmental cues causes circadian desynchrony, which is pervasive in humans. Clock misalignment can lead to various pathologies including obesity and diabetes, both of which are associated with pancreatic ductal adenocarcinoma – a devastating cancer with an 80% five-year mortality rate. Although circadian desynchrony is associated with an increased risk of several solid-organ cancers, the correlation between clock misalignment and pancreas cancer is unclear. Using a chronic jetlag model, we investigated the impact of clock misalignment on pancreas cancer initiation in mice harboring a pancreas-specific activated <i>Kras</i> mutation. We found that chronic jetlag accelerated the development of pancreatic cancer precursor lesions, with a concomitant increase in precursor lesion grade. Cell-autonomous knock-out of the clock in pancreatic epithelial cells of <i>Kras</i>-mutant mice demonstrated no acceleration of precursor lesion formation, indicating non-cell-autonomous clock dysfunction was responsible for the expedited tumor development. Therefore, we applied single-cell RNA sequencing over time and identified fibroblasts as the cell population manifesting the greatest clock-dependent changes, with enrichment of specific cancer-associated fibroblast pathways due to circadian misalignment.