DOI: 10.5061/dryad.dbrv15f7m
title: Data from: Fine-scale genetic structure in the orchid Gymnadenia conopsea is not associated with local density of flowering plants

description: Premise: Density-dependent pollinator visitation can lead to
 density-dependent mating patterns and within-population genetic structure.
 In Gymnadenia conopsea, individuals in low-density patches receive more
 self-pollen than individuals in high-density patches, suggesting higher
 relatedness at low density. Ongoing fragmentation is also expected to
 cause more local matings, potentially leading to biparental inbreeding
 depression. Methods: To evaluate whether relatedness decreases with local
 density, we analysed 1315 SNP loci in 113 individuals within two large
 populations. We quantified within-population genetic structure in one of
 the populations, recorded potential habitat barriers, and visualized gene
 flow using estimated effective migration surfaces (EEMS). We further
 estimated the magnitude of biparental inbreeding depression that would
 result from matings restricted to within 5 m. Results: There was no
 significant relationship between local density and relatedness in any
 population. We detected significant fine-scale genetic structure
 consistent with isolation-by-distance, with positive kinship coefficients
 at distances below 10 m. Kinship coefficients were low, and predicted
 biparental inbreeding depression resulting from matings within the closest
 5 m was a modest 1–3%. EEMS suggested that rocks and bushes may act as
 barriers to gene flow within a population. Conclusions: The results
 suggest that increased self-pollen deposition in sparse patches does not
 necessarily cause higher selfing rates, or that inbreeding depression
 results in low establishment success of inbred individuals. The modest
 relatedness suggests that biparental inbreeding depression is unlikely to
 be an immediate problem following fragmentation of large populations. The
 results further indicate that habitat structure may contribute to
 governing fine-scale genetic structure in G. conopsea.
description: # Data from: Fine-scale genetic structure in the orchid Gymnadenia
 conopsea is not associated with local density of flowering plants
 The excel file includes
 spatial positions and pairwise kinship coefficients estimated with SPAGeDI
 for two Öland populations of Gymnadenia conopsea ## Description of the
 data and file structure The first tab in the file is called
 'Legend', and contains the same information as given here. | Tab
 'all pairs' includes kinship coefficients for all possible
 pairwise combinations, including all individulas | |
 -------------------------------------------------------------------------------------------------------------------- | | Tab 'distance pairs' includes kinship coefficients for pairs with information on distance between individuals | | Tab 'coordinates Alb' includes spatial position of all individuals within a 50 by 30 m plot in the Alböke population | * Pop=Population [Alb=Alböke, Sto=Störlinge] * Ind=Individual ID within population [Alb=1-90, Sto=1-36] * Pair=Pair ID within population [Alb=1-45, Sto=1-18] * Kinship=pairwise kinship coefficient * Distance=pairwise spatial distance (in cm) * Density=number of flowering individuals within one square meter * X and Y=spatial coordinates of each individual within a 50 by 3 m plot in the Alböke population