cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.16946583
title: Communication Dataset from Distributed Model Predictive Control

description: This repository contains the dataset created and used for the work Reducing the Communication of Distributed Model Predictive Control: Autoencoders and Formation Control and consists of



`dataset.csv` - recorded data from inter-agent communication

`metadata.txt` - information regarding the training tasks

`plotdataset.py` - a Python script to visualize the data


The dataset consists of post-processed sequences of candidate inputs along the prediction horizon of inter-agents communication in 2000 simulated scenarios using distributed model predictive control. The dataset is stored in the csv format and the velocities alternate between linear velocity $v$ and angular velocity $\omega$ in the columns, i.e., they are stored following the prediction horizon. All scenarios used two robots in a DMPC setting. The linear velocites are denoted as `v_1` to  `v_20` and the angular velocities similarly with `omega_1` to `omega_20`. The higher the number, the further away from the current state the prediction of the input is. The metadata file `metadata.txt` contains setup information for each scenario including, for example, the number of robots, the simulation duration `tend`, and the initial state `x0` as well as the desired output. The initial state is given in absolute coordinates in the inertial frame of reference in the format $\begin{bmatrix}x_1 & y_1 & \theta_1 & x_2 & y_2 & \theta_2 \end{bmatrix}^\top$, where $x_i$ and $y_i$ are the position, and $\theta_i$ the orientation of robot $i$. The desired output consist of the absolute values for the position and orientation of the geometric center of the formation, the positions of the robots relative to the geometric center and the desired orientation of the formation.

A metadata entry has the following structure:




Number of robots: 2
x0: [-5.024750007781551, 6.088088095621365, 2.604305537257288, -6.0247588076953615, 6.088107912584617, 2.6041282948414115]
Desired state: [0.0, 0.0, 0.0, 0.5, 0.0, 0.0, -0.5, 6.123233995736766e-17, 0.0]
Prediction horizon: 20
Time: step size = 0.25, tend = 40
Weights: [1.0, 5.0, 0.1, 0.125, 0.0125]
Message timeout = 200
Optimizer: alpha = 1, beta = 0.1, sigma = 0.5
Number of Iterations per time step: 3



Plot the Candidate Inputs
The repository contains a Python script, which allows to easily visualize the candidate input sequences for each sample.

Requirements



matplotlib

pandas


To install the required packages run, for example,




pip install pandas matplotlib


 

in a terminal.


Run
To run the script, use the following command, in Linux, e.g., in bash:



python plotdataset.py <path_to_csv_file>


where `<path_to_csv_file>` is the path to the dataset file.

Running the script plots a figure with slider allowing to slide through the samples in the dataset.
DOI: 10.5281/zenodo.16946584
title: Communication Dataset from Distributed Model Predictive Control

description: This repository contains the dataset created and used for the work Reducing the Communication of Distributed Model Predictive Control: Autoencoders and Formation Control and consists of



`dataset.csv` - recorded data from inter-agent communication

`metadata.txt` - information regarding the training tasks

`plotdataset.py` - a Python script to visualize the data


The dataset consists of post-processed sequences of candidate inputs along the prediction horizon of inter-agents communication in 2000 simulated scenarios using distributed model predictive control. The dataset is stored in the csv format and the velocities alternate between linear velocity $v$ and angular velocity $\omega$ in the columns, i.e., they are stored following the prediction horizon. All scenarios used two robots in a DMPC setting. The linear velocites are denoted as `v_1` to  `v_20` and the angular velocities similarly with `omega_1` to `omega_20`. The higher the number, the further away from the current state the prediction of the input is. The metadata file `metadata.txt` contains setup information for each scenario including, for example, the number of robots, the simulation duration `tend`, and the initial state `x0` as well as the desired output. The initial state is given in absolute coordinates in the inertial frame of reference in the format $\begin{bmatrix}x_1 & y_1 & \theta_1 & x_2 & y_2 & \theta_2 \end{bmatrix}^\top$, where $x_i$ and $y_i$ are the position, and $\theta_i$ the orientation of robot $i$. The desired output consist of the absolute values for the position and orientation of the geometric center of the formation, the positions of the robots relative to the geometric center and the desired orientation of the formation.

A metadata entry has the following structure:




Number of robots: 2
x0: [-5.024750007781551, 6.088088095621365, 2.604305537257288, -6.0247588076953615, 6.088107912584617, 2.6041282948414115]
Desired state: [0.0, 0.0, 0.0, 0.5, 0.0, 0.0, -0.5, 6.123233995736766e-17, 0.0]
Prediction horizon: 20
Time: step size = 0.25, tend = 40
Weights: [1.0, 5.0, 0.1, 0.125, 0.0125]
Message timeout = 200
Optimizer: alpha = 1, beta = 0.1, sigma = 0.5
Number of Iterations per time step: 3



Plot the Candidate Inputs
The repository contains a Python script, which allows to easily visualize the candidate input sequences for each sample.

Requirements



matplotlib

pandas


To install the required packages run, for example,




pip install pandas matplotlib


 

in a terminal.


Run
To run the script, use the following command, in Linux, e.g., in bash:



python plotdataset.py <path_to_csv_file>


where `<path_to_csv_file>` is the path to the dataset file.

Running the script plots a figure with slider allowing to slide through the samples in the dataset.
DOI: 10.22002/x7hjm-zzc73
title: nanochemowt_2ndday

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-28/rawdata/</p>nanochemowt2day_atlas10028.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-28/rawdata/nanochemowt2day_atlas10028.mrc"
        > <i class="download icon"></i> Download </a></p>

        nanochemowt2day_atlas10028_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-28/3dimage_58125/nanochemowt2day_atlas10028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zqxt0-gkp82
title: pED208_pilated

description: E coli strain with plasmid pED208 overexpresses T4SS and F-like pili
description: <strong>Tilt Series Date:</strong> 2015-01-14</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were obtained from Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> o/n liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-121/rawdata/</p>pED208b_6um0021.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-121/rawdata/pED208b_6um0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED208b_6um0021_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-121/3dimage_50817/pED208b_6um0021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zyken-8a577
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-27/3dimage_71450/</p>atlas1_sq40030_full.rec,  Reconstruction (Pixel Size 1.28 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-27/3dimage_71450/atlas1_sq40030_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40030.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-27/rawdata/atlas1_sq40030.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zawy2-bry56
title: Planktonic cell of Proteus

description: Planktonic cell, PG layer, Proteus mirabillis
description: <strong>Tilt Series Date:</strong> 2015-12-26</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 195.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Purification / Growth Conditions / Treatment:</strong> OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-26-53/3dimage_71941/</p>proteus30053_part121_3.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-26-53/3dimage_71941/proteus30053_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>

        proteus30053.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-26-53/rawdata/proteus30053.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/snm87-n8363
title: pED208_pilated

description: E coli strain with plasmid pED208 overexpresses T4SS and F-like pili
description: <strong>Tilt Series Date:</strong> 2015-01-14</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were obtained from Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> o/n liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-54/rawdata/</p>pED208a0054.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-54/rawdata/pED208a0054.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED208a0054_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-54/3dimage_50682/pED208a0054_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hvq6s-ab667
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-31/3dimage_71458/</p>atlas1_sq40034_full.rec,  Reconstruction (Pixel Size 1.28 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-31/3dimage_71458/atlas1_sq40034_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40034.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-31/rawdata/atlas1_sq40034.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yd7dk-hc027
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-317/rawdata/</p>T4SSWTNov26A024_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-317/rawdata/T4SSWTNov26A024_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov26A024_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-317/3dimage_105066/T4SSWTNov26A024_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pkg5b-4qv98
title: INS-1E with Mito-DsRed

description: CLEM and ECT.
description: <strong>Tilt Series Date:</strong> 2016-07-08</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cells frozen by Zachary Freyberg at University of Pittsburgh. Collaboration established through Stephen Carter. I performed CryoLM, CLEM and ECT experiments.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-07-08-8/rawdata/</p>MitoTS7.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-07-08-8/rawdata/MitoTS7.mrc"
        > <i class="download icon"></i> Download </a></p>

        MitoTS7_forced-rot-ang78-fidless_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 210.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-07-08-8/3dimage_82094/MitoTS7_forced-rot-ang78-fidless_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2t7bw-ya789
title: T5_wt_control_Aumalemaide_5nm

description: This is a wt sample in which Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. This is a control sample. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-02-14</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-13/rawdata/</p>sq90013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-13/rawdata/sq90013.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq90013.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-13/3dimage_82415/sq90013.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/df7sb-w9715
title: PAOI delta aer2 pJN105-aer2

description: Pseudomonas aeruginosa strain PAOI delta aer2 pJN105-aer2
description: <strong>Tilt Series Date:</strong> 2015-02-12</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts (Loma Linda)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> induced pJN105-aer2, use 0.5% L-arabinoseStreak on LB plates and incubate overnight at 37°CInoculate 5 mL MOPS Minimal Media Limited Nitrogen (+ gentamicin for plasmid) from plate and grow at 30°C for ~24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-12-13/rawdata/</p>Pseudoaerplusinduced0016.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-12-13/rawdata/Pseudoaerplusinduced0016.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudoaerplusinduced0016_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-12-13/3dimage_51845/Pseudoaerplusinduced0016_full.rec"
        > <i class="download icon"></i> Download </a></p>