cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.dxm228
title: Occurrence Download

description: A dataset containing 548 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Costa Rica",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Eira barbara"
 ]
}

 The dataset includes 548 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001830-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/af260-k6c07
title: INS-1E PBC

description: Untransfected INS-1E cells
description: <strong>Tilt Series Date:</strong> 2018-07-15</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rat INS-1E pancreatic beta cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Ray Stevens</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids, stimulated with glucose.</p>
<strong>Sample Preparation:</strong> Manual Blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-14/rawdata/</p>tomography14.mrc,  Tilt Series (Pixel Size 3.26 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-14/rawdata/tomography14.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography14.rec,  Reconstruction (Pixel Size 13.04 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-14/3dimage_117031/tomography14.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography14_full.rec,  Reconstruction (Pixel Size 0.0 nm), 4.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-14/3dimage_118517/tomography14_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qnqqd-j1363
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.F30 Polara
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> mc2_155</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor  F30 Polara</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP3  M. smegmatis mc2_155.7H9 media </p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-1/rawdata/</p>kp3_1.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-1/rawdata/kp3_1.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp3_1_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-1/3dimage_27527/kp3_1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        amw2013-06-10-1_slicer16496.png,  Unambiguous REZ .  (possibly some faint flow lines) .  -andrew, 542.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/amw2013-06-10-1_slicer16496.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.fpgvnh
title: Occurrence Download

description: A dataset containing 1266 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Clematis viticella",
 "Country is France",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1266 records from 56 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001829-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/9hgh2-eze17
title: Paeruginosa_dfliG

description: These are Pseudomonas aeruginosa dfliG (flagellar motor switch protein) cells obtained from  .  the transposon library.
description: <strong>Tilt Series Date:</strong> 2019-06-23</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells are provided by Shannon Park (in Diannae Newman lab) from the transposon library.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were plated on LB agar plate with 100 ug/ml gentamicin. Then, single colony was . inoculated in LB culture (with 100 ug/ml gentamicin) at 37C overnight. After 16 hours, the cells . were added to grids and blotted.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-52/rawdata/</p>Paeruginosa_dFliG17.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-52/rawdata/Paeruginosa_dFliG17.mrc"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG17_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-52/3dimage_104226/Paeruginosa_dFliG17_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Paeruginosa_dFliG17.mrc.mdoc,  32.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-52/rawdata/Paeruginosa_dFliG17.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/27ahy-n6s90
title: Mycobacter smegmatis pneumonia

description: KP48s M. smegmatis Δ3877 (ΔeccD1)
description: <strong>Tilt Series Date:</strong> 2013-07-02</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3877</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sauton media @ 37C</p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-02-7/rawdata/</p>KP48s0007.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-02-7/rawdata/KP48s0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        KP48s0007_part121_62.rec,  Reconstruction (Pixel Size 1.68 nm), 602.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-02-7/3dimage_27876/KP48s0007_part121_62.rec"
        > <i class="download icon"></i> Download </a></p>

        amw2013-07-02-7_slicer16500.png,  REZ  (visible in spite of the fact that image reconstruction may have partially failed in this .rec file) .  -andrew, 755.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/amw2013-07-02-7_slicer16500.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5h4gd-bfa34
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP52s
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3871</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP52 M. smegmatisΔ3871   (ΔEcccA1) sauton media @ 37C</p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-27_slicer6445.jpg: Unknown membrane
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-27/rawdata/</p>kp52s0004.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-27/rawdata/kp52s0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp52s0004_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-27/3dimage_27582/kp52s0004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        amw2013-06-10-27_slicer16505.png,  REZ .  -andrew, 403.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/amw2013-06-10-27_slicer16505.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rqaqtd
title: Occurrence Download

description: A dataset containing 214 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "GadmGid is USA.37.10_1",
 "TaxonKey is one of (Crocodylia, Testudines, Squamata)"
 ]
}

 The dataset includes 214 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001831-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/kdnzx-mb447
title: HeLa cells expressing TRIM5α-YFP

description: HeLa cell line 25 expressing stable TRIM5α-YFP. Correlated light electron microscopy (PolaraSep22015). Concentration of 500 nm blue beads (phosphorex) 4/150. 20 nm gold highly concentrated. Manual blotting with Whatman 40.HeLa cell line 25 expressing stable TRIM5α-YFP.
description: <strong>Tilt Series Date:</strong> 2015-09-02</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cell line 25 and VSV-G pseudotyped virus Vpr-Tomato provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells infected with pseudovirus Vpr-Tomato (in box). 500 µl of media and 500 µl of virus. Incubated with MG-132 (Campbell et al 2008) 6hrs. Cells were washed with warm media and incubated at 37°C before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-09-02-8/3dimage_61108/</p>tomography9.rec,  Reconstruction (Pixel Size 24.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-09-02-8/3dimage_61108/tomography9.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography9.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-09-02-8/rawdata/tomography9.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography9_new.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-09-02-8/3dimage_76061/tomography9_new.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c214q-rny37
title: Saccharomyces cerevisiae - 6210

description: Raw data files of Saccharomyces cerevisiae - 6210
description: <strong>Tilt Series Date:</strong> 2017-10-25</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain from Markus Babst @ University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to ~0.6 OD from overnight cultures in YPD.</p>
<strong>Sample Preparation:</strong> added ~3.5 µL of ~0.2 OD cells for each grid and blotted from behind for ~3 sec. FIB milled on Sep25_2017.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-6/rawdata/</p>TS5.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-6/rawdata/TS5.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS5_4bin_trunc_fid_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 285.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-6/3dimage_91651/TS5_4bin_trunc_fid_DB.rec"
        > <i class="download icon"></i> Download </a></p>

        TS5_4bin_trunc_patch_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 270.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-6/3dimage_91652/TS5_4bin_trunc_patch_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/533fg-0ka82
title: hns_overexpression_PS152

description: Andrew's DNA project
description: <strong>Tilt Series Date:</strong> 2016-08-24</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown for 27h from glycerol stock. At OD 0.51 induced with ~3mM IPTG and grown for 4h and froze as such, no spinning. OD was 0.52. Cell density and distribution looked good.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-47/rawdata/</p>hnsM9_PS152_atlas10005.mrc,  Tilt Series (Pixel Size 0.6 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-47/rawdata/hnsM9_PS152_atlas10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        hnsM9_PS152_atlas10005_full.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-47/3dimage_78548/hnsM9_PS152_atlas10005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2016-08-24-47_slicer17031.png,  blue arrow: dense blob .  (the other less-dense blobs were also seen in many of the other tomograms), 572.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-47_slicer17031.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jez7m-d9k38
title: U2OS-Tubulin Tracker Green

description: U2OS cells stained with tubulin tracker to locatemidbodies using LM. Cryo-FIB milling is used to make the sample thinner.
description: <strong>Tilt Series Date:</strong> 2017-07-14</p>
<strong>Data Taken By:</strong> Wei Zhao</p>
<strong>Species / Specimen:</strong> U2OS</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-62.0°, 62.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD-patch tracking</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL.</p>
<strong>Sample Preparation:</strong> Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-07-14-7/rawdata/</p>TS8.mrc,  Tilt Series (Pixel Size 0.4569 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-07-14-7/rawdata/TS8.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS8_bin2.rec,  Reconstruction (Pixel Size 0.0 nm), 216.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-07-14-7/3dimage_86876/TS8_bin2.rec"
        > <i class="download icon"></i> Download </a></p>

        wza2017-07-14-7_slicer18631.png,  mitochondrial and autophagosome, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/wza2017-07-14-7_slicer18631.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jzmby-ehx28
title: U2OS-Tubulin Tracker Green

description: U2OS cells stained with tubulin tracker to locatemidbodies using LM. Cryo-FIB milling is used to make the sample thinner.
description: <strong>Tilt Series Date:</strong> 2017-07-15</p>
<strong>Data Taken By:</strong> Wei Zhao</p>
<strong>Species / Specimen:</strong> U2OS</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-62.0°, 62.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD-patch tracking</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL.</p>
<strong>Sample Preparation:</strong> Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-07-15-1/rawdata/</p>P_TS3.mrc,  Tilt Series (Pixel Size 0.4569 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-07-15-1/rawdata/P_TS3.mrc"
        > <i class="download icon"></i> Download </a></p>

        P_TS3_bin2.rec,  Reconstruction (Pixel Size 0.0 nm), 171.0 MB  
        
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        wza2017-07-15-1_slicer18636.png, , 1.3 MB  
        
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