cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5128681.v1
title: Supplementary Material for: Stability of Attitudes to the Ethical Issues Raised by the Return of Incidental Genomic Research Findings in Children: A Follow-Up Study

description: <b><i>Objective:</i></b> We explore the stability of parental attitudes to the ethical issues raised by the return of genomic research results. <b><i>Methods:</i></b> A 19-item questionnaire was mailed to participants in a large genome research consortium 18 months following a baseline survey. We describe the stability of parental attitudes to (a) sharing of genomic research results, (b) endorsement of children in genomic research, (c) responsibilities of researchers, and (d) responsibilities to extended family. We also explore their experience in receiving results. <b><i>Results:</i></b> Of 170 original participants, 154 (91%) responded. Most participants expressed positive rights to receive incidental genomic research findings (85%), including when ameliorative therapy was unknown (85%). Only 3% found it acceptable to delegate the decision to return results to an independent committee. Researchers, either with a parent (42%) or physician (17%), were felt to be responsible to convey research results to children when they reach adulthood. Most participants (74%) indicated that results should be shared with potentially affected extended family. These results are very similar to those of the baseline survey. All participants who received genomic results would do so again and reported actions similar to their expressed attitudes. <b><i>Conclusions:</i></b> The opinions of parents regarding genomic research remain stable over time. Guidelines on the return of results should incorporate these findings.
DOI: 10.6084/m9.figshare.5128666
title: Supplementary Material for: Downregulation of Integrins in Cancer Cells and Anti-Platelet Properties Are Involved in Holothurian Glycosaminoglycan-Mediated Disruption of the Interaction of Cancer Cells and Platelets in Hematogenous Metastasis

description: Activated platelets have been recognized as an accessory character in the cascade of tumor hematogenous metastasis, and intervention of tumor cell attachment to the activated platelets or microemboli formation might be a leading strategy to prevent tumor cells surviving in the blood vessels and sequential metastasis. Recently, we have demonstrated that holothurian glycosaminoglycan (hGAG), a sulfated polysaccharide with potent anticoagulant activity extracted from the sea cucumber <i>Holothuria leucospilota</i> Brandt, was highly efficacious against tumor metastasis. In this study, we identified the potential effects of hGAG on the disruption of interactions of cancer cells and platelets and the underlying mechanisms, which were supported by the following evidence: hGAG (1) inhibited thrombin-induced platelet activation and aggregation, (2) reduced adhesion between platelet and breast cancer cells, and abrogated platelets/cancer cells adhering to fibrinogen, (3) attenuated platelet-cancer cell complex formation (the number and size of aggregates) and (4) suppressed both mRNA and protein levels of β<sub>1</sub> and β<sub>3</sub> integrins, matrix metalloproteinase (MMP)-2 and MMP-9, while increasing the expression of the MMP inhibitor, tissue inhibitor of metalloproteinase (TIMP)-1 in MDA-MB-231 cells. These results suggested that both the antiplatelet properties and mitigation of the levels of cellular adhesion molecules contributed to the anticancer effects of hGAG, and might thus be exploited for clinical adjuvant therapy to attenuate tumor hematogenous metastasis.
DOI: 10.6084/m9.figshare.5128672
title: Supplementary Material for: Cytidine Deaminase as a Molecular Predictor of Gemcitabine Response in Patients with Biliary Tract Cancer

description: <b><i>Objective:</i></b> Gemcitabine-based chemotherapy is regarded as the standard treatment for biliary tract cancer (BTC). Potential biomarkers for gemcitabine response include the activities of cytidine deaminase (CDA), human equilibrative nucleoside transporter 1 (hENT1), deoxycytidine kinase (DCK), and ribonucleotide reductase M1 (RRM1). Here, we investigated whether single nucleotide polymorphisms (SNPs) in their encoding genes were associated with the efficacy of gemcitabine chemotherapy in treating BTC. <b><i>Methods:</i></b> We retrospectively evaluated 11 SNPs in the <i>CDA, hENT1, DCK, </i>human concentrative nucleoside transporter 3 <i>(hCNT3)</i>, and<i> RRM1</i> genes in 80 patients with unresectable, metastatic, or recurrent BTC who were treated with gemcitabine plus cisplatin. <b><i>Results:</i></b> After the results were adjusted for clinical predictors, the variant allele of rs1048977 in the <i>CDA </i>gene was associated with tumor response in a dominant model (OR, 0.23; 95% CI, 0.06-0.93; p = 0.039). No significant association was detected between the 11 SNPs and grade 3/4 toxicity. <b><i>Conclusions:</i></b> Our findings suggest that the polymorphism of <i>CDA</i> may be a potential predictive marker for the efficacy of gemcitabine-based chemotherapy in patients with BTC.
DOI: 10.6084/m9.figshare.5128675
title: Supplementary Material for: What Dyadic Reparation Is Meant to Do: An Association with Infant Cortisol Reactivity

description: <b><i>Background:</i></b> The latency to reparation of interactive mismatches (interactive repair) is argued to regulate infant distress on a psychobiological level, and maternal anxiety disorders might impair infant regulation. <b><i>Sampling and Methods:</i></b> A total of 46 dyads (19 mothers with an anxiety disorder, 27 controls) were analyzed for associations between interactive repair and infant cortisol reactivity during the Face-to-Face-Still-Face paradigm 3-4 months postpartum. Missing cortisol values (n = 16) were imputed. Analyses were conducted on both the original and the pooled imputed data. <b><i>Results:</i></b> Interactive repair during the reunion episode was associated with infant cortisol reactivity (original data: p &lt; 0.01; pooled data: p &lt; 0.01) but not maternal anxiety disorder (p &gt; 0.23). Additional stepwise regression analyses found that latency to repair during play (p &lt; 0.01), an interaction between distress during the first trimester of pregnancy and latency to repair during reunion (p &lt; 0.01) and infant self-comforting behaviors during the reunion episode (p = 0.04) made independent contributions to cortisol reactivity in the final regression model. <b><i>Conclusions:</i></b> This is the first study demonstrating that interactive repair is related to infant psychobiological stress reactivity. The lack of a relation to maternal anxiety disorder may be due to the small sample size. However, this result emphasizes that infants respond to what they experience and not to the maternal diagnostic category.
DOI: 10.6084/m9.figshare.5128669
title: Supplementary Material for: Methods in Neuroepidemiology Characterization of European Longitudinal Cohort Studies in Parkinson's Disease - Report of the JPND Working Group BioLoC-PD

description: <b><i>Background:</i></b> Enormous effort is being put into the identification and characterization of symptoms that may be used as predictive and progression markers in Parkinson's disease (PD). An impressive number of PD patients and individuals at risk for or in the prodromal stage of PD are currently followed in longitudinal studies; however, there does not exist an overview on the kind of markers evaluated and the assessments used. <b><i>Methods:</i></b> Information on the design, sample size, evaluated markers and assessments of 21 studies of the Joint Programme - Neurodegenerative Disease Research BioLoC-PD working group were collected by questionnaire. The studies were classified into at risk/prodromal or clinical PD cohorts. The assessments were grouped into quantitative assessments, investigator-rated assessments, investigator interviews, patient-rated questionnaires and caregiver-rated questionnaires. <b><i>Results:</i></b> Compilation of these data revealed an interesting consensus on evaluated markers, but there was an enormous variability of assessments. Furthermore, there is a remarkable similarity in the markers assessed and evaluation methods applied in the risk/prodromal and clinical PD cohorts. <b><i>Conclusions:</i></b> The inventory of the longitudinal cohorts that are part of the BioLoC-PD consortium reveals that there is a growing consensus on the markers that should be assessed in longitudinal cohort studies in PD. However, controversy still exists on the specific type of assessment. To allow comparison of data and common analyses it will be essential to harmonize scales and assessment outcomes.
DOI: 10.6084/m9.figshare.5128669.v1
title: Supplementary Material for: Methods in Neuroepidemiology Characterization of European Longitudinal Cohort Studies in Parkinson's Disease - Report of the JPND Working Group BioLoC-PD

description: <b><i>Background:</i></b> Enormous effort is being put into the identification and characterization of symptoms that may be used as predictive and progression markers in Parkinson's disease (PD). An impressive number of PD patients and individuals at risk for or in the prodromal stage of PD are currently followed in longitudinal studies; however, there does not exist an overview on the kind of markers evaluated and the assessments used. <b><i>Methods:</i></b> Information on the design, sample size, evaluated markers and assessments of 21 studies of the Joint Programme - Neurodegenerative Disease Research BioLoC-PD working group were collected by questionnaire. The studies were classified into at risk/prodromal or clinical PD cohorts. The assessments were grouped into quantitative assessments, investigator-rated assessments, investigator interviews, patient-rated questionnaires and caregiver-rated questionnaires. <b><i>Results:</i></b> Compilation of these data revealed an interesting consensus on evaluated markers, but there was an enormous variability of assessments. Furthermore, there is a remarkable similarity in the markers assessed and evaluation methods applied in the risk/prodromal and clinical PD cohorts. <b><i>Conclusions:</i></b> The inventory of the longitudinal cohorts that are part of the BioLoC-PD consortium reveals that there is a growing consensus on the markers that should be assessed in longitudinal cohort studies in PD. However, controversy still exists on the specific type of assessment. To allow comparison of data and common analyses it will be essential to harmonize scales and assessment outcomes.
DOI: 10.6084/m9.figshare.5128672.v1
title: Supplementary Material for: Cytidine Deaminase as a Molecular Predictor of Gemcitabine Response in Patients with Biliary Tract Cancer

description: <b><i>Objective:</i></b> Gemcitabine-based chemotherapy is regarded as the standard treatment for biliary tract cancer (BTC). Potential biomarkers for gemcitabine response include the activities of cytidine deaminase (CDA), human equilibrative nucleoside transporter 1 (hENT1), deoxycytidine kinase (DCK), and ribonucleotide reductase M1 (RRM1). Here, we investigated whether single nucleotide polymorphisms (SNPs) in their encoding genes were associated with the efficacy of gemcitabine chemotherapy in treating BTC. <b><i>Methods:</i></b> We retrospectively evaluated 11 SNPs in the <i>CDA, hENT1, DCK, </i>human concentrative nucleoside transporter 3 <i>(hCNT3)</i>, and<i> RRM1</i> genes in 80 patients with unresectable, metastatic, or recurrent BTC who were treated with gemcitabine plus cisplatin. <b><i>Results:</i></b> After the results were adjusted for clinical predictors, the variant allele of rs1048977 in the <i>CDA </i>gene was associated with tumor response in a dominant model (OR, 0.23; 95% CI, 0.06-0.93; p = 0.039). No significant association was detected between the 11 SNPs and grade 3/4 toxicity. <b><i>Conclusions:</i></b> Our findings suggest that the polymorphism of <i>CDA</i> may be a potential predictive marker for the efficacy of gemcitabine-based chemotherapy in patients with BTC.
DOI: 10.6084/m9.figshare.5128678.v1
title: Supplementary Material for: Two Novel <b><i>HOGA1</i></b> Splicing Mutations Identified in a Chinese Patient with Primary Hyperoxaluria Type 3

description: <b><i>Background:</i></b> Twenty-six <i>HOGA1</i> mutations have been reported in primary hyperoxaluria (PH) type 3 (PH3) patients with c.700 + 5G&gt;T accounting for about 50% of the total alleles. However, PH3 has never been described in Asians. <b><i>Methods:</i></b> A Chinese child with early-onset nephrolithiasis was suspected of having PH. We searched for <i>AGXT</i>, <i>GRHPR</i> and <i>HOGA1</i> gene mutations in this patient and his parents. All coding regions, including intron-exon boundaries, were analyzed using PCR followed by direct sequence analysis. <b><i>Results:</i></b> Two heterozygous mutations not previously described in the literature about <i>HOGA1</i> were identified (compound heterozygous). One mutation was a successive 2 bp substitution at the last nucleotide of exon 6 and at the first nucleotide of intron 6, respectively (c.834_834 + 1GG&gt;TT), while the other one was a guanine to adenine substitution of the last nucleotide of exon 6 (c.834G&gt;A). Direct sequencing analysis failed to find these mutations in 100 unrelated healthy subjects and the functional role on splicing of both variants found in this study was confirmed by a minigene assay based on the pSPL3 exon trapping vector. In addition, we found a SNP in this family (c.715G&gt;A, p.V239I). There were no mutations detected in <i>AGXT</i> and <i>GRHPR</i>. <b><i>Conclusion:</i></b> Two novel <i>HOGA1</i> mutations were identified in association with PH3. This is the first description and investigation on mutant gene analysis of PH3 in an Asian.
DOI: 10.6084/m9.figshare.5128657
title: Supplementary Material for: Von Freiberg nach Northampton: Der Weg des Naturheilkundigen Dr. Carl Munde in die USA

description: Der Sprachlehrer und Hydropath Dr. phil. Carl Munde wurde durch politische Umstände dazu veranlasst, nach einer bis dahin bunten Karriere seine deutsche Heimat 1849 zu verlassen, um in den USA als Leiter und Besitzer einer Kaltwasser-Heilanstalt, einer sogenannten «Water-Cure», in Florence/Northampton im US-Bundesstaat Massachusetts eineinhalb Jahrzehnte lang sein Glück zu machen. Munde war in Deutschland vor allem durch seine Schriften über die Prießnitzsche Wasserkur und entsprechende eigene Interpretationen bekannt geworden. Auch in den USA wurde sein Name weithin bekannt und ist bis heute mit dem Ortsteil Florence von Northampton verbunden. Munde, der sich als naturalisierter US-Bürger Charles Munde nannte, kehrte nach dem tragischen Verlust seiner Heilanstalt durch einen Brand im Jahr 1866 mit seiner Familie zurück nach Deutschland, um nach einer Zwischenstation in der Schweiz und einer Tätigkeit als Leiter einer Wasserheilanstalt bei Laibach (slowenisch: Ljubljana) schliesslich im damals österreichischen Görz am Isonzo seinen Alterswohnsitz zu nehmen.
DOI: 10.6084/m9.figshare.5128660
title: Supplementary Material for: The <b><i>MTHFR</i></b> C677T Polymorphism Is Related to Plasma Concentration of Oxidized Low-Density Lipoprotein in Adolescents with Cardiovascular Risk Factors

description: <b><i>Aim:</i></b> The aim of this study was to investigate possible relationships among the A1298C (rs1801131) and C677T (rs1801133) polymorphisms in the methylenetetrahydrofolate reductase <i>(MTHFR)</i> gene and levels of homocysteine, vitamins B<sub>6</sub>, B<sub>12</sub>, folic acid and lipid profile, including oxidized low-density lipoprotein (ox-LDL), of adolescents at cardiovascular risk. <b><i>Methods:</i></b> We recruited 115 adolescents (10-19 years old), 58.3% (n = 67) female, from a public school in Brazil who underwent anthropometric, biochemical and genetic tests as well as food consumption evaluation. <b><i>Results:</i></b> An important prevalence of hyperhomocysteinemia (19.1%) and alterations in triacylglycerol (17.4%), total cholesterol (26.9%) and high-density lipoprotein (HDL) cholesterol (48.0%) concentrations were observed, as well as low vitamin B<sub>6</sub> concentrations (23.5%). The categorization of homocysteine concentrations into tertiles revealed significant differences in serum concentrations of folate, vitamin B<sub>12</sub> and HDL, waist circumference and intake of total and saturated fat among the tertiles. The presence of variant alleles regarding the <i>MTHFR</i> C677T polymorphism interfered with vitamin B<sub>6</sub> and ox-LDL cholesterol concentrations. There was a trend for higher waist circumference values in T carriers (C677T), but not in C carriers (A1298C). <b><i>Conclusions:</i></b> The <i>MTHFR</i> C677T allele was associated with higher plasma vitamin B<sub>6</sub> and ox-LDL compared to the CC genotype.
DOI: 10.6084/m9.figshare.5128663
title: Supplementary Material for: Remote Ischemic Preconditioning May Attenuate Renal Ischemia-Reperfusion Injury in a Porcine Model of Supraceliac Aortic Cross-Clamping

description: <b><i>Aim:</i></b> The effect of remote ischemic preconditioning (RIPC) in decreasing renal ischemia-reperfusion injury (IRI) during a suprarenal aortic cross-clamping was examined in a swine model. <b><i>Materials and Methods:</i></b> Four groups of pigs were examined: (a) ischemia-reperfusion (IR) group, renal IRI produced by 30 min of supraceliac aortic cross-clamping; (b) RIPC I group, the same renal IRI following RIPC by brief occlusion of the infrarenal aorta (15 min ischemia and 15 min reperfusion); (c) RIPC II group, the same renal IRI following RIPC by brief occlusion of the infrarenal aorta (3 cycles of 5 min ischemia and 5 min reperfusion); (d) sham group. Renal function was assessed before and after IRI by examining creatinine, neutrophil gelatinase-associated lipocalin (NGAL), TNF-α, malondialdehyde (MDA), cystatin C and C-reactive protein (CRP) from renal vein blood samples at specific time intervals. <b><i>Results:</i></b> Both RIPC groups presented significantly less impaired results compared to the IR group when considering MDA, cystatin C, CRP and creatinine. Between the two RIPC groups, RIPC II presented a better response with regard to CRP, NGAL, TNF-α, MDA and cystatin C. <b><i>Conclusions:</i></b> Remote IR protocols and mainly repetitive short periods of cycles of IR ameliorate the biochemical kidney effects of IRI in a model of suprarenal aortic aneurysm repair.
DOI: 10.6084/m9.figshare.5128666.v1
title: Supplementary Material for: Downregulation of Integrins in Cancer Cells and Anti-Platelet Properties Are Involved in Holothurian Glycosaminoglycan-Mediated Disruption of the Interaction of Cancer Cells and Platelets in Hematogenous Metastasis

description: Activated platelets have been recognized as an accessory character in the cascade of tumor hematogenous metastasis, and intervention of tumor cell attachment to the activated platelets or microemboli formation might be a leading strategy to prevent tumor cells surviving in the blood vessels and sequential metastasis. Recently, we have demonstrated that holothurian glycosaminoglycan (hGAG), a sulfated polysaccharide with potent anticoagulant activity extracted from the sea cucumber <i>Holothuria leucospilota</i> Brandt, was highly efficacious against tumor metastasis. In this study, we identified the potential effects of hGAG on the disruption of interactions of cancer cells and platelets and the underlying mechanisms, which were supported by the following evidence: hGAG (1) inhibited thrombin-induced platelet activation and aggregation, (2) reduced adhesion between platelet and breast cancer cells, and abrogated platelets/cancer cells adhering to fibrinogen, (3) attenuated platelet-cancer cell complex formation (the number and size of aggregates) and (4) suppressed both mRNA and protein levels of β<sub>1</sub> and β<sub>3</sub> integrins, matrix metalloproteinase (MMP)-2 and MMP-9, while increasing the expression of the MMP inhibitor, tissue inhibitor of metalloproteinase (TIMP)-1 in MDA-MB-231 cells. These results suggested that both the antiplatelet properties and mitigation of the levels of cellular adhesion molecules contributed to the anticancer effects of hGAG, and might thus be exploited for clinical adjuvant therapy to attenuate tumor hematogenous metastasis.
DOI: 10.6084/m9.figshare.5128651
title: Supplementary Material for: Prognostic Impact of Placental Growth Factor on Mortality and Cardiovascular Events in Dialysis Patients

description: <b><i>Background:</i></b> Placental growth factor (PlGF), a member of the vascular endothelial growth factor (VEGF) family, has recently emerged as a predictor of survival and cardiovascular risk. Along with others, we have shown an independent association between PlGF and cardiovascular events in CKD patients, but not much is known about patients receiving dialysis. <b><i>Methods:</i></b> We studied 205 dialysis patients undergoing cardiac catheterization at the Nara Medical University between April 1, 2004, and December 31, 2012. Serum levels of PlGF and VEGF were measured with ELISA in all the patients. <b><i>Results:</i></b> During a median follow-up of 20 months, 121 participants died from any cause or experienced a cardiovascular event. In the fully adjusted analysis, having an above-median PlGF or VEGF level was associated with a hazards ratio for adverse outcomes of 2.55 (1.72-3.83) and 1.39 (0.95-2.04), respectively. Using a multimarker strategy in a model with age, serum albumin, history of coronary artery disease, brain natriuretic peptide and PlGF, patients with 2, 3 and 4 positive markers had a 3.82-, 5.77- and 6.59-fold higher risk of mortality or a cardiovascular event, respectively, compared to those with no positive markers. The model with PlGF had a significantly higher c-statistic, integrated discrimination improvement index and category-free net reclassification improvement index than the model without PlGF. <b><i>Conclusion:</i></b> PlGF is independently associated with mortality and cardiovascular events, but the association between VEGF and adverse events was attenuated with covariate adjustment. The addition of PlGF to models with established clinical predictors provides additional useful prognostic information in patients receiving dialysis.
DOI: 10.6084/m9.figshare.5128654
title: Supplementary Material for: Role of Aurora-A in Ovarian Cancer: A Meta-Analysis

description: <b><i>Background:</i></b> Recently, several studies have examined associations between Aurora-A expression and clinical outcome in patients with ovarian cancer, but yielded conflicting results with respect to survival. Therefore, the aim of this study was to evaluate the prognostic significance of Aurora-A in ovarian cancer by performing a meta-analysis. <b><i>Methods:</i></b> PubMed, Cochrane library, Web of Science, Embase, Medline and Chinese BioMed Database (CBM) databases were searched systematically and only articles in which Aurora-A expression was detected by immunohistochemical staining were included. Hazard ratios (HRs) with 95% confidence intervals (CIs) were extracted and pooled for overall survival (OS) and disease-free survival (DFS). <b><i>Results:</i></b> Our results show that the pooled HR for OS was 1.40 (95% CI 0.82-1.98, p &lt; 0.01) by univariate analysis in 7 articles (1,028) and 0.32 (95% CI 0.04-0.615, p = 0.23) by multivariate analysis in 3 studies (155). The association between Aurora-A expression and DFS was also statistically significant in 5 studies (HR = 1.14, 95% CI 0.50-1.78, p &lt; 0.01). <b><i>Conclusion:</i></b> This present meta-analysis suggests that the Aurora-A expression may be associated with poor prognosis in patients with ovarian cancer. Furthermore, studies of larger scale and well-matched regimes are warranted to confirm the findings in the future.