DOI: 10.5061/dryad.v55p5
title: Data from: Screen for mitochondrial DNA copy-number maintenance genes reveals essential role for ATP synthase

description: The machinery of mitochondrial DNA (mtDNA) maintenance is only partially
 characterized and is of wide interest due to its involvement in disease.
 To identify novel components of this machinery, plus other cellular
 pathways required for mtDNA viability, we implemented a genome-wide RNAi
 screen in Drosophila S2 cells, assaying for loss of fluorescence of mtDNA
 nucleoids stained with the DNA-intercalating agent PicoGreen. In addition
 to previously characterized components of the mtDNA replication and
 transcription machineries, positives included many proteins of the
 cytosolic proteasome and ribosome (but not the mitoribosome), three
 proteins involved in vesicle transport, some other factors involved in
 mitochondrial biogenesis or nuclear gene expression, &gt; 30 mainly
 uncharacterized proteins and most subunits of ATP synthase (but no other
 OXPHOS complex). ATP synthase knockdown precipitated a burst of
 mitochondrial ROS production, followed by copy number depletion involving
 increased mitochondrial turnover, not dependent on the canonical autophagy
 machinery. Our findings will inform future studies of the apparatus and
 regulation of mtDNA maintenance, and the role of mitochondrial
 bioenergetics and signaling in modulating mtDNA copy number.
description: Microsoft Excel table
 containing information about the images.This Excel file contains
 information about the RNAi screening, including the gene IDs corresponding
 to each well.Drosophila dsRNA_lot
 L002.xlsImages from the
 screenThis is the first part
 of a 37-part 7-zip archive. All of the files need to be downloaded to open
 the archive. A program to open it can be downloaded from
 http://www.7-zip.org/. The folder names correspond to well plates, and the
 image names give the rows and columns of the well plate (e.g. image A1.tif
 is from row A, column 1). Information about each well is given in the file
 "Drosophila dsRNA_lot L002.xls".L002.7z.001Part of image archiveL002.7z.002Part of image archiveL002.7z.003Part of image archiveL002.7z.004Part of image archiveL002.7z.005Part of image archiveL002.7z.006Part of image archiveL002.7z.007Part of image archiveL002.7z.008Part of image archiveL002.7z.009Part of image archiveL002.7z.010Part of image archiveL002.7z.011Part of image archiveL002.7z.012Part of image archiveL002.7z.013Part of image archiveL002.7z.014Part of image archiveL002.7z.015Part of image archiveL002.7z.016Part of image archiveL002.7z.017Part of image archiveL002.7z.018Part of image archiveL002.7z.019Part of image archiveL002.7z.020Part of image archiveL002.7z.021Part of image archiveL002.7z.022Part of image archiveL002.7z.023Part of image archiveL002.7z.024Part of image archiveL002.7z.025Part of image archiveL002.7z.026Part of image archiveL002.7z.027Part of image archiveL002.7z.028Part of image archiveL002.7z.029Part of image archiveL002.7z.030Part of image archiveL002.7z.031Part of image archiveL002.7z.032Part of image archiveL002.7z.033Part of image archiveL002.7z.034Part of image archiveL002.7z.035Part of image archiveL002.7z.036Part of image archiveL002.7z.037