cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22008/fk2/7dcj0u/f89i4k
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/avdhdp
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/3l7uj4
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/hkyj0m
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/kkvmap
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/wukcfx
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/euhj33
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/7rct30
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/d8yrfu
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/axkvg9
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/6oitji
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/gyxuis
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/ielryh
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/um3eah
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/8og5tm
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/mnctyh
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/adx9ag
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/9bueyk
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/chsk1a
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/nespdy
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/jsdisr
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/gnq6ki
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/xvb0yl
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/0d9g4k
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/dz4sns
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/mqyawz
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/fokvnk
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/k9vtku
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/ogz8ez
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/eq4y8i
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/sczevq
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/4f6axs
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/dnhvl5
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/yuceev
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/dowerg
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/nj05su
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/xcv0fi
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/cflc0l
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/ainqng
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/7pjqgh
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/qv2xbg
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/hzntfd
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/h7j1iw
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/p3isbg
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/8ll7js
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/boos9l
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/opfduu
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/6s3jpd
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/6mbvhh
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/kuf7md
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/wm890s
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/jh1kh5
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/3o6v88
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.34740/kaggle/dsv/3877631
title: Russian Invasion of Ukraine
DOI: 10.22008/fk2/7dcj0u/beaocg
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/7onfeu
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/lmjptp
title: snow_specific_surface_area.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/baqtos
title: SCDA_final.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/xih2ck
title: SCDA_v20.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/amvdvz
title: BBA_combination.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/1djydm
title: r_TOA_01.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/re9yt0
title: r_TOA_06.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/tcghxn
title: r_TOA_17.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/iwi60i
title: r_TOA_21.tif

description: SICE NRT
DOI: 10.22008/fk2/7dcj0u/jcinl4
title: albedo_bb_planar_sw.tif

description: SICE NRT
DOI: 10.15468/dl.hjzk78
title: Occurrence Download

description: A dataset containing 7228 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Orcinus orca (Linnaeus, 1758)"
 ]
}

 The dataset includes 7228 records from 161 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0062271-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.azetv2
title: Occurrence Download

description: A dataset containing 805 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-98.52786 -28.10660,-45.47214 -28.10660,-45.47214 -6.89340,-98.52786 -6.89340,-98.52786 -28.10660))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Pleurodema marmoratum (Duméril & Bibron, 1840)"
 ]
}

 The dataset includes 805 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0298889-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qwqdme
title: Occurrence Download

description: A dataset containing 9665 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-103.67651 -47.76346,-22.32349 -47.76346,-22.32349 -15.23654,-103.67651 -15.23654,-103.67651 -47.76346))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Drymornis bridgesii (Eyton, 1850)"
 ]
}

 The dataset includes 9665 records from 22 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0230684-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yju7se
title: Occurrence Download

description: A dataset containing 467 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lavoisiera confertiflora Naudin"
 ]
}

 The dataset includes 467 records from 40 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0365719-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rnfvsw
title: Occurrence Download

description: A dataset containing 23 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Psilocybe zapotecorum R.Heim"
 ]
}

 The dataset includes 23 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067253-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2hd7jt
title: Occurrence Download

description: A dataset containing 83 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "TaxonKey is Ainsliaea grossedentata Franch."
 ]
}

 The dataset includes 83 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0287374-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.cxuq7b
title: Occurrence Download

description: A dataset containing 89 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Ecuador",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Cyrtochilum macranthum (Lindl.) Kraenzl."
 ]
}

 The dataset includes 89 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0272119-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8crqev
title: Occurrence Download

description: A dataset containing 202 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Guibourtia tessmannii (Harms) J.Léonard"
 ]
}

 The dataset includes 202 records from 19 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0283398-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4xclg5
title: Occurrence Download

description: A dataset containing 69 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Chinese Taipei",
 "TaxonKey is Scutellaria playfairii Kudô"
 ]
}

 The dataset includes 69 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008522-180824113759888/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.e56ejq
title: Occurrence Download

description: A dataset containing 3392 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((5.875 51.425,5.925 51.425,5.925 51.475,5.875 51.475,5.875 51.425))",
 "DatasetKey is Observation.org, Nature data from around the World",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 3392 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0306901-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ymk9qy
title: Occurrence Download

description: A dataset containing 2031 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Tagetes erecta L."
 ]
}

 The dataset includes 2031 records from 74 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0038394-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ut6749
title: Occurrence Download

description: A dataset containing 108 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Argentina",
 "Geometry POLYGON((-61.10596 -36.59789,-61.23779 -37.75334,-59.39209 -38.27269,-58.29346 -38.37612,-57.10693 -38.58253,-56.31592 -37.54458,-57.72217 -36.84446,-59.47998 -36.38591,-61.10596 -36.59789))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Lepidoptera"
 ]
}

 The dataset includes 108 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0075157-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.b774vw
title: Occurrence Download

description: A dataset containing 15829 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((5.925 53.225,5.975 53.225,5.975 53.275,5.925 53.275,5.925 53.225))",
 "DatasetKey is Observation.org, Nature data from around the World",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 15829 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0306923-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.y7knqk
title: Occurrence Download

description: A dataset containing 76 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "BasisOfRecord is Specimen",
 "BasisOfRecord is Material sample"
 ]
 },
 "TaxonKey is Parnassius maharaja Avinoff, 1916"
 ]
}

 The dataset includes 76 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0074669-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qhqecz
title: Occurrence Download

description: A dataset containing 479 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Mexico",
 "Geometry POLYGON((-96.02051 18.81272,-81.95801 17.72776,-80.55176 24.1267,-84.24316 30.2211,-93.2959 30.06909,-98.56934 28.53627,-98.48145 23.4834,-96.02051 18.81272))",
 "TaxonKey is Chelonia mydas (Linnaeus, 1758)",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 479 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010169-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.en9a5y
title: Occurrence Download

description: A dataset containing 16946 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "MULTIPOLYGON(((5.241862535476685 43.294803095683314,5.241862535476685 43.25493380778211,5.340739488601685 43.25493380778211,5.340739488601685 43.294803095683314,5.241862535476685 43.294803095683314)))"
 ]
}

 The dataset includes 16946 records from 68 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0084671-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.u4h5ad
title: Occurrence Download

description: A dataset containing 12839 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((5.875 50.975,5.925 50.975,5.925 51.025,5.875 51.025,5.875 50.975))",
 "DatasetKey is Observation.org, Nature data from around the World",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 12839 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0306912-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wyrk4f
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Xanthocalanus propinquus Sars G.O., 1902"
 ]
}

 The dataset includes 4 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0134614-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5ra97d
title: Occurrence Download

description: A dataset containing 30012 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Lepus timidus Linnaeus, 1758",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 30012 records from 116 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006049-180131172636756/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.x9ugrn
title: Occurrence Download

description: A dataset containing 489799 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Poa annua L."
 ]
}

 The dataset includes 489799 records from 1156 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0084686-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/9dnz5-2x979
title: P_aeruginosa_PA14_DfliF_Krios

description: Raw data files of P_aeruginosa_PA14_DfliF_Krios
description: <strong>Tilt Series Date:</strong> 2018-09-29</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (50.0°, -50.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> I (mohammed) obtained the cells from Shannon Park (Dianne Newman lab). The cells are available in their library of Pseudomonas aeruginosa PA14.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated one colony from an LB agar plate and grew the cells overnight in LB with 100 ug/ml of Gentamycin. Then I concentrated the cells three times and mixed them with gold nanoparticles and added 3 ul on to thick carbon R2 grids and blotted them and plunge froze them (force either 6 or 3, time either 3 or 2 seconds respectively, drain time 1 second).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-21/rawdata/</p>Pseudomonas_DfliF14.mrc,  Tilt Series (Pixel Size 0.53 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-21/rawdata/Pseudomonas_DfliF14.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudomonas_DfliF14_full.rec,  Reconstruction (Pixel Size 2.12 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-21/3dimage_97169/Pseudomonas_DfliF14_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yfr6h-zd495
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-387/rawdata/</p>Hpylori_DFliF_FISE_392.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-387/rawdata/Hpylori_DFliF_FISE_392.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_392_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-387/3dimage_114252/Hpylori_DFliF_FISE_392_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4e9jb-nbx55
title: Exp phase S. acidocaldarius

description: Grid box 85 - grid1. Located dividing cells using projection images in a previous TEM session.
description: <strong>Tilt Series Date:</strong> 2018-02-04</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were harvested at 0.1 OD and kept at 4 C for further steps until freezing. Spun cells at 6000 xg for 3 min to concentrated cells to ~1 OD.</p>
<strong>Sample Preparation:</strong> Cu finder grids - glow discharged. Coated with 4 µL of 10 nm Au beads resuspended in 0.01 % BSA (7 µL of 10 nm Au + 93 µL of 0.01 % BSA) and dried. Added 3.5 µL of suspension and blotted for 2-3 sec from behind manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-02-04-8/rawdata/</p>TS8_610.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-02-04-8/rawdata/TS8_610.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS8_4bin_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-02-04-8/3dimage_102314/TS8_4bin_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>