cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5980183
title: Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator

description: ABSTRACT Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents.
DOI: 10.6084/m9.figshare.5980183.v1
title: Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator

description: ABSTRACT Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents.
DOI: 10.6084/m9.figshare.5980177
title: Equivalence Classes: Effects of the Redundant Element and the Order of Tests

description: ABSTRACT It was investigated the effect of the order of testing on equivalence class formation and stimulus control after teaching simple discriminations that included a redundant element (black spot). Nineteen college students were exposed to conditional relations training, as also testing of equivalence classes and stimulus control with mask. The order of testing varied in two experimental phases: (1) tests alternated with training, and (2) tests after training. A black spot was included in one of the compound stimuli. More accurate performances occurred when tests were conducted after training. Higher scores were related to control by S + and the S- and lower scores to multiple controls that included the black spot. These results suggest that the order of testing interact with the effects of the redundant element.
DOI: 10.6084/m9.figshare.5980177.v1
title: Equivalence Classes: Effects of the Redundant Element and the Order of Tests

description: ABSTRACT It was investigated the effect of the order of testing on equivalence class formation and stimulus control after teaching simple discriminations that included a redundant element (black spot). Nineteen college students were exposed to conditional relations training, as also testing of equivalence classes and stimulus control with mask. The order of testing varied in two experimental phases: (1) tests alternated with training, and (2) tests after training. A black spot was included in one of the compound stimuli. More accurate performances occurred when tests were conducted after training. Higher scores were related to control by S + and the S- and lower scores to multiple controls that included the black spot. These results suggest that the order of testing interact with the effects of the redundant element.
DOI: 10.6084/m9.figshare.5980174
title: Normative values for optical coherence tomography parameters in healthy children and interexaminer agreement for choroidal thickness measurements

description: ABSTRACT Purpose: To (a) determine the normative values for optical coherence tomography (OCT) parameters such as central macular thickness, retinal nerve fiber layer thickness, and choroidal thickness in healthy children; (b) investigate the relationships of these parameters with axial length, central corneal thickness, refractive errors, and intraocular pressure; and (c) determine interexaminer agreement for choroidal thickness measurements. Methods: In this cross-sectional study, 120 healthy children aged 8-15 years underwent detailed ophthalmological examination and OCT measurements. Choroidal thickness was measured at three separate locations by two independent examiners. Results: The mean global retinal nerve fiber layer thickness was 98.75 ± 9.45 μm (79.0-121.0). The mean central macular thickness was 232.29 ± 29.37 μm (190.0-376.0). The mean subfoveal choroidal thickness obtained by examiner 1 was 344.38 ± 68.83 μm and that obtained by examiner 2 was 344.04 ± 68.92 μm. Interexaminer agreement was between 99.6%-99.8% for choroidal thickness at three separate locations. Central macular thickness increased with axial length (r=0.245, p=0.007). Choroidal thickness increased with age (r=0.291, p=0.001) and decreased with axial length (r=-0.191, p=0.037). Global retinal nerve fiber layer thickness decreased with axial length (r=-0.247, p=0.007) and increased with central corneal thickness (r=0.208, p=0.022). Global retinal nerve fiber layer thickness positively correlated with choroidal thickness (r=0.354, p&lt;0.001). Global retinal nerve fiber layer thickness (r=0.223, p=0.014) and choroidal thickness (r=0.272, p=0.003) increased with the spherical equivalent (D). Conclusions: Optical coherence tomography parameters showed a wide range of variability in children. Retinal nerve fiber layer thickness, central macular thickness, and choroidal thickness were found to be either inter-related or correlated with age, central corneal thickness, axial length, and refractive errors. Furthermore, manual measurements of choroidal thickness showed high interexaminer agreement. Because normative values for optical coherence tomography parameters differed in children, the measurements should be interpreted according to an age-appropriate database.
DOI: 10.6084/m9.figshare.5980174.v1
title: Normative values for optical coherence tomography parameters in healthy children and interexaminer agreement for choroidal thickness measurements

description: ABSTRACT Purpose: To (a) determine the normative values for optical coherence tomography (OCT) parameters such as central macular thickness, retinal nerve fiber layer thickness, and choroidal thickness in healthy children; (b) investigate the relationships of these parameters with axial length, central corneal thickness, refractive errors, and intraocular pressure; and (c) determine interexaminer agreement for choroidal thickness measurements. Methods: In this cross-sectional study, 120 healthy children aged 8-15 years underwent detailed ophthalmological examination and OCT measurements. Choroidal thickness was measured at three separate locations by two independent examiners. Results: The mean global retinal nerve fiber layer thickness was 98.75 ± 9.45 μm (79.0-121.0). The mean central macular thickness was 232.29 ± 29.37 μm (190.0-376.0). The mean subfoveal choroidal thickness obtained by examiner 1 was 344.38 ± 68.83 μm and that obtained by examiner 2 was 344.04 ± 68.92 μm. Interexaminer agreement was between 99.6%-99.8% for choroidal thickness at three separate locations. Central macular thickness increased with axial length (r=0.245, p=0.007). Choroidal thickness increased with age (r=0.291, p=0.001) and decreased with axial length (r=-0.191, p=0.037). Global retinal nerve fiber layer thickness decreased with axial length (r=-0.247, p=0.007) and increased with central corneal thickness (r=0.208, p=0.022). Global retinal nerve fiber layer thickness positively correlated with choroidal thickness (r=0.354, p&lt;0.001). Global retinal nerve fiber layer thickness (r=0.223, p=0.014) and choroidal thickness (r=0.272, p=0.003) increased with the spherical equivalent (D). Conclusions: Optical coherence tomography parameters showed a wide range of variability in children. Retinal nerve fiber layer thickness, central macular thickness, and choroidal thickness were found to be either inter-related or correlated with age, central corneal thickness, axial length, and refractive errors. Furthermore, manual measurements of choroidal thickness showed high interexaminer agreement. Because normative values for optical coherence tomography parameters differed in children, the measurements should be interpreted according to an age-appropriate database.
DOI: 10.6084/m9.figshare.5980171
title: Termite Mounds Effects on Soil Properties in the Atlantic Forest Biome

description: ABSTRACT Termites have peculiar activities in the soil, inducing significant changes in the soil properties. The objective of this study was to assess physical and chemical properties and soil organic matter to evaluate the effect of termite activity and termite mounds on the soil. Two toposequences were selected and divided in slope thirds (shoulder, backslope, and footslope). In each of these, four termite mounds were selected. Samples were taken from the soils and termite mounds (top, center, and base) along with a variety of termites for identification. Analyses were carried out for physical, soil texture, and chemical properties, as well as for particle size and chemical fractioning of organic matter. The species Cornitermes cumulans was found in all mounds. Soil with termite mound presented higher clay content, acidity, and Al3+ content. Phosphorus contents differed considerably between mound material and soil. Sum of bases and cation exchange capacity of the soil were higher in mounds, and differed within the mounds, according to the sampling height. Total organic carbon and particulate carbon content were highest at the mound base. A marked disparity was observed between the contents of humic substances in the mounds and surrounding soil, with humin fraction differences in distinct topographic position. The high nutrient contents detected in the termite mounds confirm the importance of termites in concentrating nutrients.
DOI: 10.6084/m9.figshare.5980171.v1
title: Termite Mounds Effects on Soil Properties in the Atlantic Forest Biome

description: ABSTRACT Termites have peculiar activities in the soil, inducing significant changes in the soil properties. The objective of this study was to assess physical and chemical properties and soil organic matter to evaluate the effect of termite activity and termite mounds on the soil. Two toposequences were selected and divided in slope thirds (shoulder, backslope, and footslope). In each of these, four termite mounds were selected. Samples were taken from the soils and termite mounds (top, center, and base) along with a variety of termites for identification. Analyses were carried out for physical, soil texture, and chemical properties, as well as for particle size and chemical fractioning of organic matter. The species Cornitermes cumulans was found in all mounds. Soil with termite mound presented higher clay content, acidity, and Al3+ content. Phosphorus contents differed considerably between mound material and soil. Sum of bases and cation exchange capacity of the soil were higher in mounds, and differed within the mounds, according to the sampling height. Total organic carbon and particulate carbon content were highest at the mound base. A marked disparity was observed between the contents of humic substances in the mounds and surrounding soil, with humin fraction differences in distinct topographic position. The high nutrient contents detected in the termite mounds confirm the importance of termites in concentrating nutrients.
DOI: 10.6084/m9.figshare.5980165
title: Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

description: Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.
DOI: 10.6084/m9.figshare.5980165.v1
title: Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

description: Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.
DOI: 10.6084/m9.figshare.5980162
title: Risk factors and infection due to Cryptosporidium spp. in dogs and cats in southern Rio Grande do Sul

description: Abstract This study investigated the frequency of oocysts of Cryptosporidium spp. in feces from dogs and cats in five municipalities in the southern region of the state of Rio Grande do Sul. The risk factors associated with infection were also investigated. Feces samples from 110 dogs and 18 cats were stained using the auramine method. At the time of feces sampling, a questionnaire with semi-open-ended questions was applied to the animal guardians and all data obtained underwent statistical analysis. The real frequency of oocysts of Cryptosporidium spp. was 24.63% (27 dogs and two cats). Only four samples of dog feces were diarrheic and no presence of oocysts was observed in any of them. Variables that represented risk factors for infection were: homemade food, untreated water, circulation of animals on grassy terrain and living in the same environment as other animals (cattle). The results made it possible to inferring that within the population studied, the frequency of parasitism due to Cryptosporidium spp. in dogs was relevant and emphasize the asymptomatic nature of this infection. The adopting control measures are highlighted, particularly in relation to variables that represent risk factors for this infection.
DOI: 10.6084/m9.figshare.5980162.v1
title: Risk factors and infection due to Cryptosporidium spp. in dogs and cats in southern Rio Grande do Sul

description: Abstract This study investigated the frequency of oocysts of Cryptosporidium spp. in feces from dogs and cats in five municipalities in the southern region of the state of Rio Grande do Sul. The risk factors associated with infection were also investigated. Feces samples from 110 dogs and 18 cats were stained using the auramine method. At the time of feces sampling, a questionnaire with semi-open-ended questions was applied to the animal guardians and all data obtained underwent statistical analysis. The real frequency of oocysts of Cryptosporidium spp. was 24.63% (27 dogs and two cats). Only four samples of dog feces were diarrheic and no presence of oocysts was observed in any of them. Variables that represented risk factors for infection were: homemade food, untreated water, circulation of animals on grassy terrain and living in the same environment as other animals (cattle). The results made it possible to inferring that within the population studied, the frequency of parasitism due to Cryptosporidium spp. in dogs was relevant and emphasize the asymptomatic nature of this infection. The adopting control measures are highlighted, particularly in relation to variables that represent risk factors for this infection.
DOI: 10.6084/m9.figshare.5980159
title: Fruit quality attributes of low chilling requirement ‘Snowchaser’ blueberry cultivated in Brazil

description: Abstract In Brazil there are still few studies on the post-harvest quality of low-chill blueberry cultivars, which have been recently introduced in the country. ‘Snowchaser’ blueberries were evaluated during a six-day storage period regarding fruit physical and chemical properties, and its antioxidant capacity. During fruit storage there was an increased weight loss and maintenance of some skin color properties such as luminosity (L*) and b*, whereas the values of parameter a* decreased and chroma (C) values increased. The levels of anthocyanins and flavonoids in the pulp increased, as well as the antioxidant activity. Maximum fruit shelf life at room temperature was six days without reduction on the antioxidant activity along the period, which is beneficial to consumers’ health.
DOI: 10.6084/m9.figshare.5980159.v1
title: Fruit quality attributes of low chilling requirement ‘Snowchaser’ blueberry cultivated in Brazil

description: Abstract In Brazil there are still few studies on the post-harvest quality of low-chill blueberry cultivars, which have been recently introduced in the country. ‘Snowchaser’ blueberries were evaluated during a six-day storage period regarding fruit physical and chemical properties, and its antioxidant capacity. During fruit storage there was an increased weight loss and maintenance of some skin color properties such as luminosity (L*) and b*, whereas the values of parameter a* decreased and chroma (C) values increased. The levels of anthocyanins and flavonoids in the pulp increased, as well as the antioxidant activity. Maximum fruit shelf life at room temperature was six days without reduction on the antioxidant activity along the period, which is beneficial to consumers’ health.
DOI: 10.6084/m9.figshare.5980141
title: Bilingual education policies for deaf students: contributions to academic literacy in higher education

description: ABSTRACT The systematic enrollment of deaf students in higher education in the last decade demanded important institutional changes regarding the right to a bilingual education, that is, to provide access and production of knowledge in Brazilian sign language and in Portuguese in written form. In this sense, this article aims to discuss the process of bilingual education of deaf students in higher education, presenting actions developed within the Universidade Federal do Paraná (Federal University of Paraná, UFPR), highlighting the contributions brought to the process of bilingual academic literacy in undergraduate and post-graduate programs. We understand that the focus attributed to the figure of the interpreter of Brazilian sign language and the specialized educational service in the national policy of inclusive education does not respond to the needs of adult working deaf students who reach higher education with difficulties in reading and writing Portuguese and little significant experiences in sign language. As a positive result, we point out the contributions to the bilingual academic literacy of deaf students, the development of a specific methodology for the elaboration of bilingual material - in different textual genres - and the amplification of the translator's performance references in the inclusion process. The inclusive process consists in dialogue-based interaction between researchers and specialized professionals, with focus on the protagonism of deaf students, subjects of the educational policy, in the planning of actions and decisions that involve their access and permanence at UFPR.