DOI: 10.6084/m9.figshare.30814493
title: The tiff files of "<b>Spatiotemporal Characterization of Single-Stranded DNA Intermediates after UV Irradiation:</b><b><i> </i></b><b>II. Effects of </b><b><i>recA</i></b><b> and </b><b><i>recJ</i></b>"

description: Supporting raw tiff data of:<br>Spatiotemporal Characterization of Single-Stranded DNA Intermediates after UV Irradiation: II. Effects of recA and recJRemy A. A. Ripandelli1, Elizabeth A. Wood2, Andrew Robinson1, Antoine M. van Oijen1, and Michael M. Cox2*1Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, Australia2College of Agricultural and Life Sciences, Department of Biochemistry, University of Wisconsin-Madison, Wisconsin, USA Short title: Disruption of postreplication gap processing dynamics in recJ mutants*Correspondence should be addressed to this author. Current address: College of Agricultural and Life Sciences, Department of Biochemistry, University of Wisconsin-Madison, Wisconsin, USA. Email: cox@biochem.wisc.edu<br>This dataset provides a representative subset of the raw fluorescence microscopy data used to study postreplication gap formation and repair in Escherichia coli following UV irradiation at 5 J/m². Because the full dataset comprises hundreds of gigabytes of time-lapse microscopy files, this Figshare deposit includes 2–3 example positions per strain. Each example corresponds to a single microfluidic chip position and contains the complete time-lapse recording from that position. For every position, both the pre-UV treatment time-lapse and its corresponding post-UV time-lapse are provided.Imaging was performed using two excitation wavelengths: 568 nm for visualizing fluorescently labeled cells and 458 nm for detecting SSB-based fluorescent gap-marker signals. The folder structure reflects the experimental layout. At the top level, the pre-UV and post-UV directories each contain subfolders for every E. coli strain included in the subset. Within each strain, subfolders correspond to individual microfluidic chip positions, each housing the full time series of:- 568 nm channel TIFF files (cell visualization),- 458 nm channel TIFF files (SSB gap-marker fluorescence), and- CSV files containing automated foci-detection results for the corresponding 458 nm frames.Note that these tiff files are already corrected for the laser beam profile.<br>Although this is a curated subset rather than the full dataset, these examples accurately represent the imaging workflow, channel configuration, and biological behaviors observed in the complete data collection. The time-lapses capture UV-induced increases in SSB-marked gaps, their resolution in repair-proficient strains, and the long-lived, high-intensity foci characteristic of recF, recO, and recA mutants. They also illustrate RecJ-dependent gap enlargement in RecFOR-deficient backgrounds and SSB-rich repair intermediates that accumulate in strains lacking RecA or related factors.This subset enables transparency, reproducibility, and methodological clarity while keeping file sizes manageable for Figshare. The full multi-hundred-gigabyte dataset remains available from the corresponding author upon reasonable request.