DOI: 10.5061/dryad.7c7b561
title: Data from: TSPO PET Using [18F]PBR111 reveals persistent neuroinflammation following acute diisopropylfluorophosphate intoxication in the rat

description: Acute intoxication with organophosphates (OPs) can trigger status
 epilepticus followed by persistent cognitive impairment and/or
 electroencephalographic abnormalities. Neuroinflammation is widely posited
 to influence these persistent neurological consequences. However, testing
 this hypothesis has been challenging, in part because traditional
 biometrics preclude longitudinal measures of neuroinflammation within the
 same animal. Therefore, we evaluated the performance of non-invasive
 positron emission tomography (PET), using the translocator protein (TSPO)
 radioligand [18F]PBR111 against classic histopathologic measures of
 neuroinflammation in a preclinical model of acute intoxication with the OP
 diisopropylfluorophosphate (DFP). Adult male Sprague Dawley rats
 administered pyridostigmine bromide (0.1 mg/kg, im) 30 min prior to
 administration of DFP (4 mg/kg, sc), atropine sulfate (2 mg/kg, im) and
 2-pralidoxime (25 mg/kg, im) exhibited moderate-to-severe seizure
 behavior. TSPO PET performed prior to DFP exposure and at 3, 7, 14, 21 and
 28 days post-exposure (DPE) revealed distinct lesions, as defined by
 increased standardized uptake values (SUV). Increased SUV showed high
 spatial correspondence to immunohistochemical evidence of
 neuroinflammation, which was corroborated by cytokine gene and protein
 expression. Regional SUV metrics varied spatiotemporally with DPE and
 correlated with the degree of neuroinflammation detected
 immunohistochemically. Furthermore, SUV metrics were highly correlated
 with seizure severity, suggesting that early termination of OP-induced
 seizures may be critical for attenuating subsequent neuroinflammatory
 responses. Normalization of SUV values to a cerebellar reference region
 improved correlations to all outcome measures and seizure severity.
 Collectively, these results establish TSPO PET using [18F]PBR111 as a
 robust, non-invasive tool for longitudinal monitoring of neuroinflammation
 following acute OP intoxication.
description: Anatomic_T2w_MRI_DFP&VehicleThis .zip file contains T2-weighted MRI scans used for anatomic registration of the corresponding PET data sets. Data is stored in Analyze format. Additional information for each animal (eg: treatment) can be found in the meta data spreadsheet.AnatomicT2wMRI.zip18FPBR111PET_01of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_02of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_03of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_04of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_05of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_06of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_07of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_08of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_09of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_10of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_11of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_12of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_13of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_14of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_15of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.18FPBR111PET_16of16This .zip file, one of sixteen parts (see file name), contains dynamic PET scans of DFP or vehicle exposed rats. Data is stored in raw format (4 byte little endian float) with image parameters stored within a header file. Additional information for each animal (eg: treatment, injected radiotracer dose, weight) can be found in the meta data spreadsheet.Scan_Meta_Data_SpreadsheetThis .csv file contains details required for analysis and interpretation of [18F]PBR111 PET data. Specifically: treatment group, animal weight at the time of scanning, and injected radiotracer dose.LuminexResultsHippocampusPiriformCortex_PCRResultsThis .xlsx file contains results from multiplex immunoassays for cytokine protein levels and PCR assessment of cytokine gene expression. Data sets are differentiated by the naming of spreadsheet tabs. The associated README file contains information regarding materials and methods.