DOI: 10.5061/dryad.c68cj62
title: Data from: Lower bumblebee colony reproductive success in agricultural compared to urban environments

description: Urbanisation represents a rapidly growing driver of land-use change. While
 it is clear that urbanisation impacts species abundance and diversity,
 direct effects of urban land-use on animal reproductive success are rarely
 documented. Here we show that urban land-use is linked to long-term colony
 reproductive output in a key pollinator. We reared colonies from
 wild-caught bumblebee (Bombus terrestris) queens, placed them at sites
 characterised by varying degrees of urbanisation from inner city to rural
 farmland, and monitored the production of sexual offspring across the
 entire colony cycle. Our land-use cluster analysis identified three site
 categories, and this categorization was a strong predictor of colony
 performance. Crucially, colonies in the two clusters characterized by
 urban development produced more sexual offspring than those in the cluster
 dominated by agricultural land. These colonies also reached higher peak
 size, had more food stores, encountered fewer parasite invasions and
 survived for longer. Our results show a link between urbanisation and
 bumblebee colony reproductive success, supporting the theory that urban
 areas provide a refuge for pollinator populations in an otherwise barren
 agricultural landscape.
description: ASamuelson_UrbanBumblebee_ColonyDataColony totals data file with data collected from field. Lat = Latitude; Lon = Longitude; Col = Colony identifier; Site = site name; Crith_suc & Apic_suc = Crithidia bombi and Apicystis bombi positive samples; Crith_fail & Apic_fail = Crithidia bombi and Apicystis bombi negative samples; Syntretus, Crithidia, Apicystis = parasitised (binary); Countave = peak no. workers; Tot_cuck = number of B. vestalis invasions; Cu_bin = B. vestalis invasions (binary), Tot_male = toal males; Tot_gyne = total gynes; Tot_rep = total males + gynes; G/M_thorave = gyne/male mean thorax width (mm); G/M_wmass = gyne/male mean wet mass (g); G/M_dmass = gyne/male mean dry mass (g); Q_week = week queen left experiment due to death or colony moribundity; Q_died = queen died (binary); Col_death_week = week colony became moribund; Col_status = status for survival analysis; Rep_wk = Week colony produced reproductives or became moribund; Rep_status = produced reproductives (binary); Ave_temp = average temperature (C); Ave_hum = average humidity (%); Sum_prec = total rainfall during experiment (mm); LU750, LU500, LU250 & LU100 = 750m, 500m, 250m & 100m radius clustered land-use variables; Prop_... = Proportion land-use variables for each radius; ...PC1 = Principle component scores for each radius.ASamuelson_UrbanBumblebee_WeekDataWeekly colony measurements data file with data collected from field. Week = week number; Date = date; Time= time; Col = Colony identifier; Site = site name; Site.ID = unique colony; Tot_cuck = number of B. vestalis invasions; Cu_bin = B. vestalis invasions (binary); LU750, LU500, LU250 & LU100 = 750m, 500m, 250m & 100m radius clustered land-use variables; . Lat = Latitude; Lon = Longitude; Removed = colony moribund; Count1/2/3 = Repeated worker counts; Countave = mean worker count; Q_larv = gyne larvae count; Q_pup = gyne pupae count; Q_open = emerged gyne pupae count; Q_alive = queen alive (binary); Usurper = usurper B. terrestris present (binary); Cuck_A = alive B. vestalis; Cuck_I = injured B. vestalis; Cuck_d = dead B. vestalis; Cuck_tot = total B. vestalis; ET_clos = closed egg towers; ET_open = open egg towers; Male = male count; gyne = gyne count; Dead_W = dead worker count; Pollen/Nectar = pollen/nectar present in pots (binary); Wax_cover = wax cover (N = none, P = partial, F = full); Weight = colony weight (kg); Scale_malf = scale malfunction (binary); Ave_temp = average temperature (C); Ave_hum = average humidity (%); Sum_prec = total rainfall during experiment (mm); Prop_... = Proportion land-use variables for each radius; ...PC1 = Principle component scores for each radius.ASamuelson_UrbanBumblebee_FatData.csvFat analysis data file with data collected from bee samples analysed in the lab, with sheets for males, gynes and workers. Week = week number; Date = date; Col = Colony identifier; Site = site name; Sitewk = Site and week; no = bee number; Thor1/2/3 = repeated thorax width measurements (mm); Thor_ave = mean thorax width (mm); Wet_wt_whole = wet mass whole bee (g); Dry_wt_whole = dry mass whole bee (g); Dry_wt_ab_BE = dry mass abdomen before ether (g); Dry_wt_ab_AE = dry mass abdomen after ether (g); Fat_wt_g = Fat mass (g); Fat_wt_mg = Fat mass (mg); Fat_mg.mm = fat mg/mm; Prop_fat = proportion fat content