cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22828240
title: Immunoexpression profile of hypoxia-inducible factor (HIF) targets in potentially malignant and malignant oral lesions: a pilot study

description: Abstract Oral potentially malignant disorders (OPMD) are associated with an increased risk of oral squamous cell carcinoma (OSCC). OSCC has an aggressive profile and is the most prevalent among different head and neck malignancies. Most OSCC patients are diagnosed with advanced stage tumors and have a poor prognosis. Cancer cells are able to reprogram their metabolism, even in the presence of oxygen, enhancing the conversion of glucose to lactate via the glycolytic pathway, a phenomenon mainly regulated by hypoxia-inducible factor (HIF) signaling. Thus, several glycometabolism-related biomarkers are upregulated. Objectives This study aimed to evaluate the immunoexpression of the HIF targets GLUT1, GLUT3, HK2, PFKL, PKM2, pPDH, LDHA, MCT4, and CAIX in OPMD and OSCC samples, in order to identify potential correlations between biomarkers’ immunoexpression, clinicopathological features, and prognostic parameters. Methodology OSCC and OPMD samples from 21 and 34 patients (respectively) were retrospectively collected and stained for the different biomarkers by immunohistochemistry. Results CAIX and MCT4 expressions were significantly higher in OSCC samples when compared with OPMD samples, while the rest were also expressed by OPMD. GLUT3 and PKM2 alone, and the concomitant expression of more than four glycometabolism-related biomarkers were significantly correlated with the presence of dysplasia in OPMD. When considering OSCC cases, a trend toward increased expression of biomarkers and poor clinicopathological features was observed, and the differences regarding HK2, PFKL, LDHA and MCT4 expression were significant. Moreover, HK2 and CAIX were correlated with low survival rates. GLUT1 and GLUT3 were significantly associated with poor outcome when their expression was observed in the hypoxic region of malignant lesions. Conclusion OPMD and OSCC cells overexpress glycolysis-related proteins, which is associated with aggressive features and poor patient outcome. Further research is needed to deeply understand the glycolic phenotype in the process of oral carcinogenesis.
DOI: 10.6084/m9.figshare.22828240.v1
title: Immunoexpression profile of hypoxia-inducible factor (HIF) targets in potentially malignant and malignant oral lesions: a pilot study

description: Abstract Oral potentially malignant disorders (OPMD) are associated with an increased risk of oral squamous cell carcinoma (OSCC). OSCC has an aggressive profile and is the most prevalent among different head and neck malignancies. Most OSCC patients are diagnosed with advanced stage tumors and have a poor prognosis. Cancer cells are able to reprogram their metabolism, even in the presence of oxygen, enhancing the conversion of glucose to lactate via the glycolytic pathway, a phenomenon mainly regulated by hypoxia-inducible factor (HIF) signaling. Thus, several glycometabolism-related biomarkers are upregulated. Objectives This study aimed to evaluate the immunoexpression of the HIF targets GLUT1, GLUT3, HK2, PFKL, PKM2, pPDH, LDHA, MCT4, and CAIX in OPMD and OSCC samples, in order to identify potential correlations between biomarkers’ immunoexpression, clinicopathological features, and prognostic parameters. Methodology OSCC and OPMD samples from 21 and 34 patients (respectively) were retrospectively collected and stained for the different biomarkers by immunohistochemistry. Results CAIX and MCT4 expressions were significantly higher in OSCC samples when compared with OPMD samples, while the rest were also expressed by OPMD. GLUT3 and PKM2 alone, and the concomitant expression of more than four glycometabolism-related biomarkers were significantly correlated with the presence of dysplasia in OPMD. When considering OSCC cases, a trend toward increased expression of biomarkers and poor clinicopathological features was observed, and the differences regarding HK2, PFKL, LDHA and MCT4 expression were significant. Moreover, HK2 and CAIX were correlated with low survival rates. GLUT1 and GLUT3 were significantly associated with poor outcome when their expression was observed in the hypoxic region of malignant lesions. Conclusion OPMD and OSCC cells overexpress glycolysis-related proteins, which is associated with aggressive features and poor patient outcome. Further research is needed to deeply understand the glycolic phenotype in the process of oral carcinogenesis.
DOI: 10.6084/m9.figshare.22828241
title: The Antonio Gonçalves Peryassú mosquito collection at the Museu Nacional, in Rio de Janeiro: retrieving the memory of lost heritage

description: Abstract Collections formed and studies conducted in the early decades of the twentieth century in Rio de Janeiro, Brazil, were crucial for the study of the systematization and natural history of mosquitoes in Brazil. One key figure in this context was Antonio Gonçalves Peryassú. The history of a collection he organized at Museu Nacional [National Museum] in Rio de Janeiro between 1918 and 1922 is analyzed.
DOI: 10.6084/m9.figshare.22828241.v1
title: The Antonio Gonçalves Peryassú mosquito collection at the Museu Nacional, in Rio de Janeiro: retrieving the memory of lost heritage

description: Abstract Collections formed and studies conducted in the early decades of the twentieth century in Rio de Janeiro, Brazil, were crucial for the study of the systematization and natural history of mosquitoes in Brazil. One key figure in this context was Antonio Gonçalves Peryassú. The history of a collection he organized at Museu Nacional [National Museum] in Rio de Janeiro between 1918 and 1922 is analyzed.
DOI: 10.6084/m9.figshare.22828243
title: Association between the Phytochemical Index and Risk Factors for Cardiovascular Disease in Adults

description: Abstract Background There are few publications about the association between the phytochemical index (PI) and risk factors for cardiovascular disease. Objective To evaluate the association between the PI and risk factors for cardiovascular disease in adults. Methods This was a cross-sectional study with 141 adults, between 20 and 59 years of age. We analyzed lifestyle (physical activity), sociodemographic data (sex, age), anthropometric data (body mass index [BMI], waist circumference [WC]), biochemical data (lipid profile, blood glucose), food consumption, and phytochemical intake (expressed as PI = daily energy derived from phytochemical-rich foods ÷ total daily energy intake × 100). We performed bivariate analyses (Spearman’s correlation) and multiple linear regression adjusted for potential confounders, considering p &lt; 0.05 as significant. Results The median PI was 9.80 (interquartile range: 11.45). PI was inversely correlated with BMI (rs: −0.43) and WC (rs: −0.36) and positively correlated with high-density lipoprotein cholesterol (HDL-cholesterol) (rs: 0.25), all with p &lt; 0.05. In multiple regression analysis, PI was inversely associated with BMI (B: −0.08; 95%CI: −0.15, −0.01) after adjusting for total energy, sex, age, and physical activity; and positively associated with HDL-cholesterol (Model 1 adjusted for total energy, sex, age, and physical activity [B: 0.21; 95% CI: 0.02, 0.41]; Model 2 adjusted for BMI, sex, age, and physical activity [B: 0.21; 95% CI: 0.008, 0.40]; Model 3 adjusted for WC, sex, age, and physical activity [B: 0.20; 95%CI: 0.01, 0.40]), all with p &lt; 0.05. Conclusion The results have demonstrated that higher phytochemical intake, expressed by PI, was inversely associated with BMI and positively associated with HDL-cholesterol.
DOI: 10.6084/m9.figshare.22828243.v1
title: Association between the Phytochemical Index and Risk Factors for Cardiovascular Disease in Adults

description: Abstract Background There are few publications about the association between the phytochemical index (PI) and risk factors for cardiovascular disease. Objective To evaluate the association between the PI and risk factors for cardiovascular disease in adults. Methods This was a cross-sectional study with 141 adults, between 20 and 59 years of age. We analyzed lifestyle (physical activity), sociodemographic data (sex, age), anthropometric data (body mass index [BMI], waist circumference [WC]), biochemical data (lipid profile, blood glucose), food consumption, and phytochemical intake (expressed as PI = daily energy derived from phytochemical-rich foods ÷ total daily energy intake × 100). We performed bivariate analyses (Spearman’s correlation) and multiple linear regression adjusted for potential confounders, considering p &lt; 0.05 as significant. Results The median PI was 9.80 (interquartile range: 11.45). PI was inversely correlated with BMI (rs: −0.43) and WC (rs: −0.36) and positively correlated with high-density lipoprotein cholesterol (HDL-cholesterol) (rs: 0.25), all with p &lt; 0.05. In multiple regression analysis, PI was inversely associated with BMI (B: −0.08; 95%CI: −0.15, −0.01) after adjusting for total energy, sex, age, and physical activity; and positively associated with HDL-cholesterol (Model 1 adjusted for total energy, sex, age, and physical activity [B: 0.21; 95% CI: 0.02, 0.41]; Model 2 adjusted for BMI, sex, age, and physical activity [B: 0.21; 95% CI: 0.008, 0.40]; Model 3 adjusted for WC, sex, age, and physical activity [B: 0.20; 95%CI: 0.01, 0.40]), all with p &lt; 0.05. Conclusion The results have demonstrated that higher phytochemical intake, expressed by PI, was inversely associated with BMI and positively associated with HDL-cholesterol.
DOI: 10.6084/m9.figshare.22828244
title: Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation

description: Abstract Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments.
DOI: 10.6084/m9.figshare.22828244.v1
title: Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation

description: Abstract Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments.
DOI: 10.6084/m9.figshare.22828246
title: Selectivity of post-emergence herbicides in soybean and their efficacy on the control of Conyza spp.

description: ABSTRACT The management of Conyza spp. is becoming increasingly challenging in soybean, especially post-emergence; therefore, it must be linked to the effectiveness of control with selectivity. This study aimed to evaluate the phytotoxic effects on soybean and the control of Conyza spp. at an advanced phenological stage through different post-emergence herbicides registered for soybean. The experimental design was randomized blocks with plots divided in time with 4 replications, consisting of 14 treatments: cloransulam at three different doses (30, 35, and 40 g a.i. ha-1); fomesafen (250 g a.i. ha-1); imazethapyr (100 g a.i. ha-1); chlorimuron in three different doses (15, 18, and 20 g a.i. ha-1); flumetsulam (108 g a.i. ha-1); lactofen (180 g a.i. ha-1); bentazon (720 g a.i. ha-1); and flumiclorac (60 g a.i. ha-1). All were associated with glyphosate (1080 g a.i. ha-1), in addition to two controls, one weeded and the other without. Visual assessments of phytotoxicity in the soybean crop and weed control were performed at 7, 14, 21, 28, and 35 days after treatment (DAT). Bentazon stood out among all treatments, showing adequate selectivity in soybean, in addition to effective weed control at 14 DAT. No dose of cloransulam or chlorimuron herbicide was effective in controlling Conyza spp. The phenological stage of Conyza spp. and water availability impacted weed control of post-emergence herbicides and selectivity in soybean.
DOI: 10.6084/m9.figshare.22828246.v1
title: Selectivity of post-emergence herbicides in soybean and their efficacy on the control of Conyza spp.

description: ABSTRACT The management of Conyza spp. is becoming increasingly challenging in soybean, especially post-emergence; therefore, it must be linked to the effectiveness of control with selectivity. This study aimed to evaluate the phytotoxic effects on soybean and the control of Conyza spp. at an advanced phenological stage through different post-emergence herbicides registered for soybean. The experimental design was randomized blocks with plots divided in time with 4 replications, consisting of 14 treatments: cloransulam at three different doses (30, 35, and 40 g a.i. ha-1); fomesafen (250 g a.i. ha-1); imazethapyr (100 g a.i. ha-1); chlorimuron in three different doses (15, 18, and 20 g a.i. ha-1); flumetsulam (108 g a.i. ha-1); lactofen (180 g a.i. ha-1); bentazon (720 g a.i. ha-1); and flumiclorac (60 g a.i. ha-1). All were associated with glyphosate (1080 g a.i. ha-1), in addition to two controls, one weeded and the other without. Visual assessments of phytotoxicity in the soybean crop and weed control were performed at 7, 14, 21, 28, and 35 days after treatment (DAT). Bentazon stood out among all treatments, showing adequate selectivity in soybean, in addition to effective weed control at 14 DAT. No dose of cloransulam or chlorimuron herbicide was effective in controlling Conyza spp. The phenological stage of Conyza spp. and water availability impacted weed control of post-emergence herbicides and selectivity in soybean.
DOI: 10.6084/m9.figshare.22828247
title: Body condition factor and diet the endemic ichthyofauna in streams of the Atlantic Forest inside and outside in an environmental protection area

description: ABSTRACT This is paper we analyze the diet and body condition factor (Mi) of three endemic species inside and outside an environmental protection area, to compare the effectiveness of the protection area in providing a greater spectrum of food items and greater environmental well-being to ichthyofauna. The study area comprised streams of the Ribeira do Iguape River basin, Campos Gerais National Park (CGNP), Paraná state and its surroundings. Eleven streams were sampled inside and outside, using electric fishing. A Permutational Multivariate Analysis of Variance to investigate the composition of food items and a Permutational Analysis of Multivariate Dispersions was used to investigate the variability between food items. The Scaled Mass Index (Mi) of the body condition was used to determine the relative condition factor of each individual of each species. Regarding the diet, there were no significant differences between inside and outside in the composition and variability of food items, this similarity may be associated with existing environmental degradation inside. However, the Mi was significant, and only one species presented greater well-being inside, because inside streams receive greater allochthonous intake of riparian vegetation, but, when it comes to the two species that perform the link of the aquatic food web with the terrestrial, the opposite occurred. These results indicate that the studied fragment, under anthropic pressures, is currently unable to conserve its biodiversity. The inside has no Management Plan, the manager must elaborate and implement it as a first step so that the situation can be reverted and the conservation effectiveness ensured in this highly threatened area, characterized by being one of the most important remnants of the Atlantic Forest, having relevant ecosystem services from a rare humid forest of Araucaria.
DOI: 10.6084/m9.figshare.22828247.v1
title: Body condition factor and diet the endemic ichthyofauna in streams of the Atlantic Forest inside and outside in an environmental protection area

description: ABSTRACT This is paper we analyze the diet and body condition factor (Mi) of three endemic species inside and outside an environmental protection area, to compare the effectiveness of the protection area in providing a greater spectrum of food items and greater environmental well-being to ichthyofauna. The study area comprised streams of the Ribeira do Iguape River basin, Campos Gerais National Park (CGNP), Paraná state and its surroundings. Eleven streams were sampled inside and outside, using electric fishing. A Permutational Multivariate Analysis of Variance to investigate the composition of food items and a Permutational Analysis of Multivariate Dispersions was used to investigate the variability between food items. The Scaled Mass Index (Mi) of the body condition was used to determine the relative condition factor of each individual of each species. Regarding the diet, there were no significant differences between inside and outside in the composition and variability of food items, this similarity may be associated with existing environmental degradation inside. However, the Mi was significant, and only one species presented greater well-being inside, because inside streams receive greater allochthonous intake of riparian vegetation, but, when it comes to the two species that perform the link of the aquatic food web with the terrestrial, the opposite occurred. These results indicate that the studied fragment, under anthropic pressures, is currently unable to conserve its biodiversity. The inside has no Management Plan, the manager must elaborate and implement it as a first step so that the situation can be reverted and the conservation effectiveness ensured in this highly threatened area, characterized by being one of the most important remnants of the Atlantic Forest, having relevant ecosystem services from a rare humid forest of Araucaria.
DOI: 10.6084/m9.figshare.22828249
title: On the genus Diphya: notes on the Brazilian D. bicolor and a new species from Ecuador (Araneae: Tetragnathidae)

description: ABSTRACT In this paper a new species of Diphya Nicolet, 1849, D. napo sp. nov. is described based on males and females from Ecuador. The female of Diphya bicolor Vellard, 1926 is described and illustrated for the first time and new records are presented. Oarces ornatus Mello-Leitão, 1935 and Azilia eximia (Mello-Leitão, 1940) are synonymized with D. bicolor, being A. eximia removed from the synonym of Azilia Keyserling, 1881 and being Cardimia Mello-Leitão, 1940 considered synonym of Diphya.
DOI: 10.6084/m9.figshare.22828249.v1
title: On the genus Diphya: notes on the Brazilian D. bicolor and a new species from Ecuador (Araneae: Tetragnathidae)

description: ABSTRACT In this paper a new species of Diphya Nicolet, 1849, D. napo sp. nov. is described based on males and females from Ecuador. The female of Diphya bicolor Vellard, 1926 is described and illustrated for the first time and new records are presented. Oarces ornatus Mello-Leitão, 1935 and Azilia eximia (Mello-Leitão, 1940) are synonymized with D. bicolor, being A. eximia removed from the synonym of Azilia Keyserling, 1881 and being Cardimia Mello-Leitão, 1940 considered synonym of Diphya.
DOI: 10.6084/m9.figshare.22828252
title: Organochlorine and organophosphate pesticides in soils of a vulnerable area from an aquifer in Northern Colombia

description: ABSTRACT The Morroa aquifer, which is an essential source of water supply in Sucre, Colombia, with more than 500,000 users, may be at risk because of pesticide contamination that comes from different agricultural activities. The aim of this research was to evaluate the relationship between the presence of pesticides and the physicochemical properties of soils in the most vulnerable areas of the Morroa aquifer. Therefore, farmers were surveyed about pesticide usage and soil samples which were taken for analysis of pesticide and physicochemical properties. The results demonstrated that soils in these areas were relatively homogeneous in terms of texture, pH, cation exchange capacity and organic matter content. Pesticides detected in these soils were malathion, β-BCH, p-p’DDE, m-p’DDD, p-p’DDT, endosulfan, endrincetone, chlorpyrifos, dieldrin, endosulfan-sulfate, heptachlor-epoxide, and parathion. Physicochemical properties such as pH and texture of soils and intrinsic characteristics of the pesticide were found as the most influential variables according to statistical results. This study also presents a map of the spatial distribution of pesticide concentration according to the concentration of pesticides, which constitutes an important tool for the planning of agricultural activities that supports decision-making and the implementation of measures to mitigate potential impacts caused by pesticide pollution.
DOI: 10.6084/m9.figshare.22828252.v1
title: Organochlorine and organophosphate pesticides in soils of a vulnerable area from an aquifer in Northern Colombia

description: ABSTRACT The Morroa aquifer, which is an essential source of water supply in Sucre, Colombia, with more than 500,000 users, may be at risk because of pesticide contamination that comes from different agricultural activities. The aim of this research was to evaluate the relationship between the presence of pesticides and the physicochemical properties of soils in the most vulnerable areas of the Morroa aquifer. Therefore, farmers were surveyed about pesticide usage and soil samples which were taken for analysis of pesticide and physicochemical properties. The results demonstrated that soils in these areas were relatively homogeneous in terms of texture, pH, cation exchange capacity and organic matter content. Pesticides detected in these soils were malathion, β-BCH, p-p’DDE, m-p’DDD, p-p’DDT, endosulfan, endrincetone, chlorpyrifos, dieldrin, endosulfan-sulfate, heptachlor-epoxide, and parathion. Physicochemical properties such as pH and texture of soils and intrinsic characteristics of the pesticide were found as the most influential variables according to statistical results. This study also presents a map of the spatial distribution of pesticide concentration according to the concentration of pesticides, which constitutes an important tool for the planning of agricultural activities that supports decision-making and the implementation of measures to mitigate potential impacts caused by pesticide pollution.