DOI: 10.5061/dryad.mgqnk998t
title: Data from: Enhanced competitive advantage of invasive plants by growth-defense trade-off: Evidence from phytohormone metabolism and transcriptomic analysis

description: Invasive plants often exhibit a competitive advantage over native species,
 posing a serious threat to them. Phytohormone signaling can regulate the
 entire process of invasive plant growth and defense in ways that have
 largely unexplored for invaders in competitive conditions. Here, we
 planted an invasive species, Flaveria bidentis L., and three native
 species (Setaria viridis, Artemisia annua, and Xanthium sibiricum) as
 monocultures (two seedlings each) or mixtures (one F. bidentis with one
 native) in the greenhouse, then employed phytohormone metabolomics and
 transcriptomics to investigate the changes in hormone content and their
 gene expression patterns in F. bidentis during competitive processes with
 native plants. Our results revealed that during competition processes with
 three native species, the levels of growth-promoting hormones, auxin (IAA)
 and gibberellins (GA3), significantly increased in F. bidentis. These
 hormone increases were accompanied by the key up-regulation of genes in
 the auxin and gibberellin signaling pathways (e.g., ARF and GID1), which
 effectively spurred increases in plant height and biomass. Conversely, the
 levels of defense-related hormones, jasmonic acid (JA) and salicylic acid
 (SA), were significantly reduced, along with the down-regulation of genes
 in their signaling pathways, including COI1 and TGA. These decreases in
 defense-related hormones and gene expression alleviated the antagonistic
 effects on growth-promoting hormones, enabling F. bidentis to grow more
 rapidly and enhance its competitive advantage. Synthesis and applications.
 Our findings reveal that trade-offs between growth and defense hormone
 signaling pathways enhance the competitive advantage of invasive alien
 species over native ones. This insight into the invasive success of exotic
 species provides a foundation for developing targeted control strategies.
 By specifically regulating hormone signaling pathways, the competitiveness
 of invasive species could be dampened, thereby mitigating the threat to
 native ecosystems.
description: # Enhanced competitive advantage of invasive plants by growth-defense
 trade-off: Evidence from phytohormone metabolism and transcriptomic
 analysis
 [https://doi.org/10.5061/dryad.mgqnk998t](https://doi.org/10.5061/dryad.mgqnk998t) ## Description of the data and file structure This dataset contains nine .xlsx files, each focusing on different plant parameters. The files are named and structured as follows: Files and variables ##### File: ##### File 1: Data1\_Plant height : This file records plant height measurements * **Variable**: Plant Height - **Unit**: Centimeters (cm) ##### File 2: Data2\_Total biomass: This file records plant biomass measurements * **Variable**: Biomass * **Unit**: Grams (g) ##### File 3: Data3\_Relative growth rate: This file records plant growth rate measurements * **Variable**: Growth Rate * **Unit**: Milligrams per day (mg/day) ##### File 4: Data4\_Auxin content: This file records plant auxin content * **Variable**: Auxin content * **Unit**: Nanograms per gram (ng/g) ##### File 5: Data5\_Gibberellin content: This file records plant gibberellin content * **Variable**: Gibberellin content * **Unit**: Nanograms per gram (ng/g) ##### File 6: Data6\_Jasmonic acid content: This file records plant jasmonic acid content * **Variable**: Jasmonic content * **Unit**: Nanograms per gram (ng/g) ##### File 7: Data7\_Salicylic acid content: This file records plant salicylic acid content * **Variable**: Salicyc content * **Unit**: Nanograms per gram (ng/g) ##### File 8: Data8\_Transcriptome: This file records plant gene expression * **Variable**: Gene Expression - **Unit**: Fragments Per Kilobase of transcript per Million mapped fragments (FPKM) - Column 1:Gene ID Column 2-7: Gene expression (FPKM) Column 8: log2FoldChange Column 9: p-value Column 10: Regulation ##### File 9: Data9\_qRT-PCR: This file contains quantified gene expression levels measured by qRT-PCR * **Variable**: Gene Expression - **Unit**: Relative Quantification (2^(-ΔΔCt)) **Description:**  **Abbreviations:** Fm: *F. bidentis* monoculture. Sm: *S. viridis* monoculture. Am: *A. annua* monoculture. Xm: *X. sibiricum *monoculture. F/F:S：*F. bidentis* in the mixture between *F. bidenti*s and *S. viridis*. S/F:S: *S. viridis* in the mixture between *F. bidentis* and *S. viridis*. F/F:A: *F. bidentis* in the mixture between *F. bidentis* and *A. annua*. A/F:A: *A. annua* in the mixture between *F. bidentis* and *A. annua*. F/F:X: *F. bidentis* in the mixture between F. bidentis and *X. sibiricum*. X/F:X: X. sibiricum in the mixture between F. bidentis and *X. sibiricum* The overall variables used in the dataset are as follows: * Plant height (cm) - Total biomass (g) * Relative growth rate (mg/day) - auxin content (ng/g) * Gibberellin content (ng/g) - Jasmonic acid content (ng/g) * Salicylic acid content (ng/g) - log2FoldChange of gene expression * Relative Expression ( 2^(-ΔΔCt)) ## Access information Other publicly accessible locations of the data: * No Data was derived from the following sources: * no