cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3886/e239456
title: HIFLD OPEN Landmark Areas (USACE IENC)

description: The USACE IENCs coverage area consists of 7,260 miles across 21 rivers primarily located in the Central United States. IENCs apply to inland waterways that are maintained for navigation by USACE for shallow-draft vessels (e.g., maintained at a depth of 9-14 feet, dependent upon the waterway project authorization). Generally, IENCs are produced for those commercially navigable waterways which the National Oceanic and Atmospheric Administration (NOAA) does not produce Electronic Navigational Charts (ENCs). However, Special Purpose IENCs may be produced in agreement with NOAA.
description: Archived from the HIFLD Open GIS data repository, which was deactivated on August 26, 2025. (Downloaded 08/26)
DOI: 10.3886/e239456v1
title: HIFLD OPEN Landmark Areas (USACE IENC)

description: The USACE IENCs coverage area consists of 7,260 miles across 21 rivers primarily located in the Central United States. IENCs apply to inland waterways that are maintained for navigation by USACE for shallow-draft vessels (e.g., maintained at a depth of 9-14 feet, dependent upon the waterway project authorization). Generally, IENCs are produced for those commercially navigable waterways which the National Oceanic and Atmospheric Administration (NOAA) does not produce Electronic Navigational Charts (ENCs). However, Special Purpose IENCs may be produced in agreement with NOAA.
description: Archived from the HIFLD Open GIS data repository, which was deactivated on August 26, 2025. (Downloaded 08/26)
DOI: 10.6075/j0tx3d1r
title: CIL:37257, Neurospora crassa. In Cell Image Library

description: An electron micrograph of a thin section of a hypha of the fungal ascomycete Neurospora crassa. The cell wall, mitochondria and cytoplasmic ribosomes are prominent. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Reference Luck, D.J.L. 1963. Formation of mitochondria in Neurospora crassa: A quantitative radioautographic study. J. Cell Biol. 16:483-499. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 20,000X.
description: Preparation: chemically fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Processing history: unprocessed raw data
DOI: 10.6075/j0qj7gbd
title: CIL:7625, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A high resolution image of the detail of cell surface structures. The cell is covered by the plasma membrane and outer alveolar sac membrane which is separated from the inner alveolar sac membrane by an extensive alveolar space. Cilia extend through this alveolus to the true cell surface. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0vq31v6
title: CIL:18453, damselfly, flight muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: unembedded tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: Most images show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. This image is part of the group image CFA00512. TEM magnification 8100X. The negative film was scanned at LBL then reduced in size for upload. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer. pH 7.2. stored at 4C for several days. Muscle dissected out. Infiltrated in 30% glycerol in water. Frozen in liquid nitrogen cooled Freon, fractured in Balzers 400 Freeze-fracture, shadowed with platinum at 45o angle. Replicas were cleaned with Clorox (to remove tissue and with either sulfuric acid or NaOH to try removing the tracheolae. Replicas examined in a Philips 410 Microscope. Reference for these images: 
Takekura, H. and Franzini-Armstrong C. The structure of Ca2+ release units in arthropod body muscle indicates an indirect mechanism for excitation-contraction coupling. Biophys. J. 83, 83, 2742-2753, 2002.
DOI: 10.6075/j0qr4w7z
title: CIL:10249, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print scanned for Photoshop
Data qualification: Processed
description: The DNA replication band in macronucleus of Halteria. Like hypotrichs the macronucleus of this oligotrich has a DNA replication band where DNA is synthesized. The nucleolus is evident. TEM taken on 3/9/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7601) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0q81cn5
title: CIL: 52806, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: One z layer of a z-stack at the given tile position
description: Tile x=1, y=3, z=4 from PS11_167511L_Myc_Terc
DOI: 10.6084/m9.figshare.30498419
title: original data Ne Zha 2 Overseas Chinese Primary Students Survey

description: This study investigates the psychological and cultural mechanisms underlying the cross-cultural communication of the Chinese animated film Ne Zha: The Demon Boy Churns the Sea (hereafter Ne Zha 2) among Chinese primary school students living in New Zealand. Drawing upon Hofstede’s theory of cultural dimensions, the research constructs and empirically tests a structural model to explore how cognitive, emotional, and behavioral factors interact to shape communication outcomes. Based on 253 valid questionnaire responses, the findings reveal that Ne Zha 2 achieved a high viewing rate (98.02%) but only moderate communication intention (M = 3.366), indicating a coexistence of “high exposure and moderate willingness to share.” The relative importance of influencing factors was ranked as follows: comprehension level (β=0.210), viewing habits (β=0.200), cultural cognition (β=0.157), emotional resonance (β=0.156), and language barriers (β=−0.160). Furthermore, significant differences were observed across gender, age, duration of residence, and birthplace. The results demonstrate that Hofstede’s cultural dimensions exert both direct and indirect effects on cross-cultural media reception through mediating psychological variables. This research provides new empirical evidence for understanding cultural adaptation and identity negotiation in transnational media communication and contributes to the broader discussion on cultural psychology and global media effects.
DOI: 10.6084/m9.figshare.30498419.v1
title: original data Ne Zha 2 Overseas Chinese Primary Students Survey

description: This study investigates the psychological and cultural mechanisms underlying the cross-cultural communication of the Chinese animated film Ne Zha: The Demon Boy Churns the Sea (hereafter Ne Zha 2) among Chinese primary school students living in New Zealand. Drawing upon Hofstede’s theory of cultural dimensions, the research constructs and empirically tests a structural model to explore how cognitive, emotional, and behavioral factors interact to shape communication outcomes. Based on 253 valid questionnaire responses, the findings reveal that Ne Zha 2 achieved a high viewing rate (98.02%) but only moderate communication intention (M = 3.366), indicating a coexistence of “high exposure and moderate willingness to share.” The relative importance of influencing factors was ranked as follows: comprehension level (β=0.210), viewing habits (β=0.200), cultural cognition (β=0.157), emotional resonance (β=0.156), and language barriers (β=−0.160). Furthermore, significant differences were observed across gender, age, duration of residence, and birthplace. The results demonstrate that Hofstede’s cultural dimensions exert both direct and indirect effects on cross-cultural media reception through mediating psychological variables. This research provides new empirical evidence for understanding cultural adaptation and identity negotiation in transnational media communication and contributes to the broader discussion on cultural psychology and global media effects.
DOI: 10.6075/j0r210sn
title: CIL: 50731, Drosophila melanogaster. In Cell Image Library

description: Item type: Digital
Imaging mode: CM12 TEM equipped with AMT digital camera
description: Specimen preparation: Larval brains (LBs) were prepared from starved (amino acid-restricted) first instar (L1) larvae to enrich for quiescent neural stem cells. The LBs were glutaraldehyde-fixed, tannic acid and OsO4-stained, embedded in resin, thin-sectioned (60-70 nm) and stained with uranyl acetate/lead citrate, then observed by TEM.
description: We show in this study that brain and intestinal stem cells of Drosophila contain a new type of cytoplasmic protrusion which is enriched in mitochondria and that the mitochondria are probably dependent on microtubules for their distribution. The protrusions form mitochondria-enriched cytoplasmic bridges from the stem cells to their niche that we speculate are important in regulating stem cell quiescence and activation.
DOI: 10.6075/j0rr1xxh
title: CIL: 53955. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: Visualization methods: confocal microscopy
description: RGC family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
DOI: 10.6075/j0571b0x
title: CIL:35722, Saccharomyces cerevisiae. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Images were acquired using a 60x 1.42 NA objective lens on a Delta Vision system equipped with a CoolSNAP_HQ2 / HQ2-ICX285. Please read the microscopy section of the referenced paper for details before image analysis.
description: This is a time lapse image series of Saccharomyces cerevisiae expressing GFP tagged Bub2, a GTPase activator involves in mitotic cell cycle spindle orientation checkpoint and regulation of exit from mitosis. These phase and GFP images are part of an image group containing CIL 35720-35739. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.
DOI: 10.6075/j0kh0mbt
title: CIL:7807, saccharomyces cerevisiae. In Cell Image Library

description: This animation shows a nucleosome located in its putative binding site on the SWI-SNF remodeling complex from yeast. The atomic structure of the DNA component of the nucleosome based on x-ray diffraction (red and green) is shown, together with the 3D envelope of SWI-SNF derived from cryo electron microscopy (gold). The location was inferred from crosslinking and other studies (see the PMID reference). The animation shows rotation about an arbitrary vertical axis. Rotation about the horizontal axis is shown in the grouped animation.
description: SWI-SNF structure was obtained from TEM images of material negatively stained with uranyl acetate, single particle reconstruction techniques used to derive the 3D volume, which was then thresholded to provide a surface representation. Different angular views of the 3D surface were used to create the movie.
See: Smith CL et al. 2003 Structural analysis of the yeast SWI/SNF chromatin remodeling complex.
Nat Struct Biol.10:141-5.
description: Item type: animation; color graphic
Processing history: Animation based on 3D envelope
DOI: 10.6075/j06m3695
title: CIL: 51949, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library

description: A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding drug slowly in 1/4 increments over 90 minutes.
description: Imaging mode: DICM
Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective
description: ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C

Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files:
1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html)
2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/)
3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory
4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK"
5. The 16 bit image will typically look dark and will require brightness adjustment.
6. In the ImageJ toolbar, select Image &gt;&gt; Adjust &gt;&gt; Brightness/Contrast and select "Auto", or manually set the brightness and contrast.
DOI: 10.6075/j02807b6
title: CIL: 54122. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: Visualization methods: confocal microscopy
description: TK family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
DOI: 10.6075/j0qf8sx3
title: CIL:35881, Saccharomyces cerevisiae. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Lte1. 
 
 LTE1 is essential for termination of M phase at low temperatures, along with TEM1, and CDC15, all part of the mitotic exit network which is an elaborate signaling system comprising at least eight essential genes: CDC5, CDC14, CDC15, DBF2, DBF20, LTE1, MOB1, and TEM1. LTE1 was originally discovered as a locus essential for growth at 8 C, and was named for this trait as Low Temperature Essential 1. It was later inferred from sequence similarities that LTE1 must code for a guanine nucleotide exchange factor. Genetic evidence suggests that Lte1p activates the GTPase Tem1p which activates the protein kinase Cdc15p. Cdc15p then relieves the inhibition of the protein phosphatase Cdc14p by Net1p, thereby allowing exit from mitosis. In a normal cell cycle, Bub2p bound to Tem1p restrains mitotic exit until the daughter-bound centrosome (with which both Bub2p and Tem1p preferentially associate) comes into contact with Lte1p in the bud, effectively coupling mitotic exit with nuclear segregation. Some evidence suggests that the spatial separation of Tem1p and Lte1p is a key mechanism for controlling the timing of mitotic exit.
 
 These phase,gfp images are part of an image group that ranges from CIL:35880-35899. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
DOI: 10.6075/j0t43scv
title: CIL:1449, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data
description: Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:1448
DOI: 10.5281/zenodo.17490888
title: Peer Pings and Self-Control: WhatsApp Group Norms, Short- Video Algorithms, and Risky Adolescent Behaviour in Indonesia

description: This dataset comprises survey and trace-based measures from 1024 Indonesian secondary students nested within 52 classes across 18 schools, including multi-item scales of self-control, WhatsApp-based peer injunctive and descriptive norms, and risky online/offline behaviors, alongside smartphone-derived notification density and detailed short-video platform usage (TikTok/Reels/Shorts, autoplay status, and algorithmic-feed share). Variables are organized for multilevel analysis (student/class), with item-level Likert responses and constructed indices (e.g., algorithmic exposure composite), enabling CFA and MSEM to examine how peer norms, self-control, and algorithmic curation jointly predict adolescent risk outcomes.