cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19905116.v1
title: Influence of caries activity and number of saliva donors: mineral and microbiological responses in a microcosm biofilm model

description: Abstract Objective this study evaluated the mineral and microbiological response of biofilms originating from different types of saliva inoculum with distinct levels of caries activity. Methodology the biofilms grown over enamel specimens originated from saliva collected from a single donor or five donors with two distinct levels of caries activity (caries-active and caries-free) or from pooling saliva from ten donors (five caries-active and five caries-free). The percentage surface hardness change (%SHC) and microbiological counts served as outcome variables. Results the caries activity of donors did not affect the %SHC values. Inoculum from five donors compared to a single donor showed higher %SHC values (p=0.019). Higher lactobacilli counts were observed when saliva from caries-active donors was used as the inoculum (p=0.017). Pooled saliva from both caries activity levels showed higher mutans streptococci counts (p&lt;0.017). Conclusion Overall, pooled saliva increased the mineral response of the derived biofilms, but all the inoculum conditions formed cariogenic biofilms and caries lesions independently of caries activity.
DOI: 10.6084/m9.figshare.19905121
title: Armed stem to stinger: a review of the ecological roles of scorpion weapons

description: Abstract Scorpions possess two systems of weapons: the pincers (chelae) and the stinger (telson). These are placed on anatomically and developmentally well separated parts of the body, that is, the oral appendages and at the end of the body axis. The otherwise conserved body plan of scorpions varies most in the shape and relative dimensions of these two weapon systems, both across species and in some cases between the sexes. We review the literature on the ecological function of these two weapon systems in each of three contexts of usage: (i) predation, (ii) defense and (iii) sexual contests. In the latter context, we will also discuss their usage in mating. We first provide a comparative background for each of these contexts of usage by giving examples of other weapon systems from across the animal kingdom. Then, we discuss the pertinent aspects of the anatomy of the weapon systems, particularly those aspects relevant to their functioning in their ecological roles. The literature on the functioning and ecological role of both the chelae and the telson is discussed in detail, again organized by context of usage. Particular emphasis is given on the differences in morphology or usage between species or higher taxonomic groups, or between genders, as such cases are most insightful to understand the roles of each of the two distinct weapon systems of the scorpions and their evolutionary interactions. We aimed to synthesize the literature while minimizing conjecture, but also to point out gaps in the literature and potential future research opportunities.
DOI: 10.6084/m9.figshare.19905121.v1
title: Armed stem to stinger: a review of the ecological roles of scorpion weapons

description: Abstract Scorpions possess two systems of weapons: the pincers (chelae) and the stinger (telson). These are placed on anatomically and developmentally well separated parts of the body, that is, the oral appendages and at the end of the body axis. The otherwise conserved body plan of scorpions varies most in the shape and relative dimensions of these two weapon systems, both across species and in some cases between the sexes. We review the literature on the ecological function of these two weapon systems in each of three contexts of usage: (i) predation, (ii) defense and (iii) sexual contests. In the latter context, we will also discuss their usage in mating. We first provide a comparative background for each of these contexts of usage by giving examples of other weapon systems from across the animal kingdom. Then, we discuss the pertinent aspects of the anatomy of the weapon systems, particularly those aspects relevant to their functioning in their ecological roles. The literature on the functioning and ecological role of both the chelae and the telson is discussed in detail, again organized by context of usage. Particular emphasis is given on the differences in morphology or usage between species or higher taxonomic groups, or between genders, as such cases are most insightful to understand the roles of each of the two distinct weapon systems of the scorpions and their evolutionary interactions. We aimed to synthesize the literature while minimizing conjecture, but also to point out gaps in the literature and potential future research opportunities.
DOI: 10.6084/m9.figshare.19905136
title: A correlation study between a corneal endothelial count in patients with different stages of keratoconus using specular microscopy

description: ABSTRACT Objective: To evaluate the corneal endothelial count in patients with keratoconus (KCN) by specular microscopy and correlate with the stage of keratoconus. Methods: Ninety-three eyes from 61 patients with KCN were included in this cross-sectional study. The eyes were classified into KCN stages 1 to 4 according to the Amsler-Krumeich classification using keratometry obtained by corneal topography and pachymetry readings obtained by specular microscopy. Results: Age ranged from 12 to 43 years, mean ± (standard deviation) 22.1 ± 6.7 years. The average keratometry ranged from 42.25 to 71.4 D, (53.0 ± 6.1 D). Pachymetry ranged from 350 to 606 µm, (461.7 ± 47.1 µm). Regarding the Amsler classification, 23 patients (24.7%) had stage 1, 24 (25.8%) stage 2, 5 (6.5%) stage 3 and 41 patients (44.1%) stage 4. No linear correlation was observed between mean keratometry and endothelial cell count (Pearson's correlation coefficient = -0.05). In the early to moderate stages of KCN, the mean endothelial cell count was 2738.3 ± 285.4 cell / mm2, while in the advanced KCN group (stages 3 and 4) it was 2670.6 ± 262.7 cell / mm2 , p = 0.24. Conclusions: No correlation was found between the endothelial cell count and the KCN stage.
DOI: 10.6084/m9.figshare.19905136.v1
title: A correlation study between a corneal endothelial count in patients with different stages of keratoconus using specular microscopy

description: ABSTRACT Objective: To evaluate the corneal endothelial count in patients with keratoconus (KCN) by specular microscopy and correlate with the stage of keratoconus. Methods: Ninety-three eyes from 61 patients with KCN were included in this cross-sectional study. The eyes were classified into KCN stages 1 to 4 according to the Amsler-Krumeich classification using keratometry obtained by corneal topography and pachymetry readings obtained by specular microscopy. Results: Age ranged from 12 to 43 years, mean ± (standard deviation) 22.1 ± 6.7 years. The average keratometry ranged from 42.25 to 71.4 D, (53.0 ± 6.1 D). Pachymetry ranged from 350 to 606 µm, (461.7 ± 47.1 µm). Regarding the Amsler classification, 23 patients (24.7%) had stage 1, 24 (25.8%) stage 2, 5 (6.5%) stage 3 and 41 patients (44.1%) stage 4. No linear correlation was observed between mean keratometry and endothelial cell count (Pearson's correlation coefficient = -0.05). In the early to moderate stages of KCN, the mean endothelial cell count was 2738.3 ± 285.4 cell / mm2, while in the advanced KCN group (stages 3 and 4) it was 2670.6 ± 262.7 cell / mm2 , p = 0.24. Conclusions: No correlation was found between the endothelial cell count and the KCN stage.
DOI: 10.6084/m9.figshare.19905139
title: Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury

description: Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect.
DOI: 10.6084/m9.figshare.19905139.v1
title: Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury

description: Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect.
DOI: 10.18112/openneuro.ds004134.v1.0.0
title: Experience Sampling in Resting State
DOI: 10.6084/m9.figshare.19905148
title: Effect of different Pilates workout endings on the kyphotic and lumbar lordotic angles

description: Abstract Introduction: There are no studies that investigate whether different workout endings of the Pilates method (PM), either prioritizing the activation of powerhouse muscles or the relaxation of muscles trained during the session, affects spinal curvature. Objective: Identify the immediate effect of a PM session that ends with soft tissue mobilization or wall exercises on the kyphotic and lumbar lordotic angles. Methods: A crossover clinical trial with 19 female Pilates practitioners that used a Flexicurve ruler to evaluate thoracic and lumbar spine curvature before and after a PM exercise protocol, and after endings that involved soft tissue mobilization using massage balls or wall exercises. The order of the different endings was chosen by random draw and the study volunteers participated in two interventions, with intervals of seven to ten days between them. Two two-way ANOVAs were performed (one for each postural variable), using the factors “assessment time” (pre-session; post-PE; post-ending) and “type of ending” (soft tissue or wall exercises). Results: There was no significant difference between the assessment times or endings for the kyphotic (p = 0.972; p = 0.747) and lumbar lordotic angles (p = 0.574; p = 0.627), respectively. Conclusion: Pilates instructors can opt for more relaxing or more active endings without affecting the kyphotic and lumbar lordotic angles.
DOI: 10.6084/m9.figshare.19905148.v1
title: Effect of different Pilates workout endings on the kyphotic and lumbar lordotic angles

description: Abstract Introduction: There are no studies that investigate whether different workout endings of the Pilates method (PM), either prioritizing the activation of powerhouse muscles or the relaxation of muscles trained during the session, affects spinal curvature. Objective: Identify the immediate effect of a PM session that ends with soft tissue mobilization or wall exercises on the kyphotic and lumbar lordotic angles. Methods: A crossover clinical trial with 19 female Pilates practitioners that used a Flexicurve ruler to evaluate thoracic and lumbar spine curvature before and after a PM exercise protocol, and after endings that involved soft tissue mobilization using massage balls or wall exercises. The order of the different endings was chosen by random draw and the study volunteers participated in two interventions, with intervals of seven to ten days between them. Two two-way ANOVAs were performed (one for each postural variable), using the factors “assessment time” (pre-session; post-PE; post-ending) and “type of ending” (soft tissue or wall exercises). Results: There was no significant difference between the assessment times or endings for the kyphotic (p = 0.972; p = 0.747) and lumbar lordotic angles (p = 0.574; p = 0.627), respectively. Conclusion: Pilates instructors can opt for more relaxing or more active endings without affecting the kyphotic and lumbar lordotic angles.
DOI: 10.6084/m9.figshare.19905152
title: “Lessons for teachers:” the Health Education (Hygiene and Preventive Medicine) course and teacher training in Rio de Janeiro, 1929-1930

description: Abstract This article analyzes the course in health education (Hygiene and Preventive Medicine) developed in 1929 by Oscar Clark, head doctor of the Medical Inspection and School Dental Care Service. We investigate the concept of teacher training implemented in schools in Rio de Janeiro (then Brazil’s capital) to create a “health crusade.” We start from the understanding that the course emerged in locus and produced lessons on education and hygiene, acting as a prescriptive space for medicine, pedagogy, and science, remembering that the course was part of the strategies of the Board of Instruction (1927-1930) to establish a normative and educational ethos for everyday teaching practices.
DOI: 10.6084/m9.figshare.19905152.v1
title: “Lessons for teachers:” the Health Education (Hygiene and Preventive Medicine) course and teacher training in Rio de Janeiro, 1929-1930

description: Abstract This article analyzes the course in health education (Hygiene and Preventive Medicine) developed in 1929 by Oscar Clark, head doctor of the Medical Inspection and School Dental Care Service. We investigate the concept of teacher training implemented in schools in Rio de Janeiro (then Brazil’s capital) to create a “health crusade.” We start from the understanding that the course emerged in locus and produced lessons on education and hygiene, acting as a prescriptive space for medicine, pedagogy, and science, remembering that the course was part of the strategies of the Board of Instruction (1927-1930) to establish a normative and educational ethos for everyday teaching practices.
DOI: 10.17632/m53xds7yvt.1
title: EXPERIMENTAL RECORD-Slug Attenuation

description: Two phase Flow Loop Experimental Data recordings obtained during Slug flow attenuation experiment as described in the included schematic.
the raw data, the summary and its corresponding graphs and flow regime map are included in the excel file.
The recorded data show that using a closed end pipe as a dampening volume for the slug flow the recorded data, show that dampening volumes are effective in reducing the slug severity.
DOI: 10.6084/m9.figshare.19905154
title: Perception about aspects of the disease and its treatment in patients with glaucoma

description: ABSTRACT Objective: Compare, through structured questionnaires, the knowledge about disease, management of eye drops and adherence to treatment of glaucoma patients disposed in two groups according to educational levels and socioeconomic levels. Methods: A cross-sectional analytical study was carried out applying structured questionnaires based on an exploratory study to assess the level of Glaucoma patients' knowledge relationated with the disease in two different audiences: the single health system (SUS) and private health plans. The questionnaires were used by doctors residents in Ophthalmology. A sample was composed of 202 patients among which 100 were attended by SUS and the others 102 patients were holders of private health plans. All questionnaires have a free and informed consent form signed by the participant and the responsible researcher. Results: Patients were divided into two groups, consisting of: 100 SUS patients and 102 private health plans. The results revealed that: 58.6% of SUS patients had incomplete elementary schooling and 25.5% of private health insurance patients had some level of higher education; 49% of the SUS group had an income with less than 2 minimum wages while the health insurance group presented 39.4% with more than 4 minimum wages (p &lt;0.001); 51.5% of the SUS group has no spending on eye drops and 67.4% of the health insurance group spends more than R $ 30.00 (p &lt;0.001) so, 77% of the SUS group receives financial aid and 52.5% of the health insurance group does not receive any financial support (p &lt;0.001); 63.6% of the health insurance group believes that the bigger amount of instillations than eyedrops does not improve glaucoma, while approximately 50% of the SUS group reports that there is an improvement when increasing instillations or has no idea (p = 0.030); Both groups obtained a similar level of general knowledge of the disease, with no statistical difference. Conclusion: We conclude that regardless of educational and socioeconomic level if prevails a good doctor-patient relationship, in addition to close monitoring, it is possible to transmit adequate knowledge about the disease, increasing levels of treatment adherence.
DOI: 10.6084/m9.figshare.19905154.v1
title: Perception about aspects of the disease and its treatment in patients with glaucoma

description: ABSTRACT Objective: Compare, through structured questionnaires, the knowledge about disease, management of eye drops and adherence to treatment of glaucoma patients disposed in two groups according to educational levels and socioeconomic levels. Methods: A cross-sectional analytical study was carried out applying structured questionnaires based on an exploratory study to assess the level of Glaucoma patients' knowledge relationated with the disease in two different audiences: the single health system (SUS) and private health plans. The questionnaires were used by doctors residents in Ophthalmology. A sample was composed of 202 patients among which 100 were attended by SUS and the others 102 patients were holders of private health plans. All questionnaires have a free and informed consent form signed by the participant and the responsible researcher. Results: Patients were divided into two groups, consisting of: 100 SUS patients and 102 private health plans. The results revealed that: 58.6% of SUS patients had incomplete elementary schooling and 25.5% of private health insurance patients had some level of higher education; 49% of the SUS group had an income with less than 2 minimum wages while the health insurance group presented 39.4% with more than 4 minimum wages (p &lt;0.001); 51.5% of the SUS group has no spending on eye drops and 67.4% of the health insurance group spends more than R $ 30.00 (p &lt;0.001) so, 77% of the SUS group receives financial aid and 52.5% of the health insurance group does not receive any financial support (p &lt;0.001); 63.6% of the health insurance group believes that the bigger amount of instillations than eyedrops does not improve glaucoma, while approximately 50% of the SUS group reports that there is an improvement when increasing instillations or has no idea (p = 0.030); Both groups obtained a similar level of general knowledge of the disease, with no statistical difference. Conclusion: We conclude that regardless of educational and socioeconomic level if prevails a good doctor-patient relationship, in addition to close monitoring, it is possible to transmit adequate knowledge about the disease, increasing levels of treatment adherence.
DOI: 10.6084/m9.figshare.19905160
title: Bioactivity effects of extracellular matrix proteins on apical papilla cells

description: Abstract Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology Different concentrations (1, 5, and 10 µg/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5×103 cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; α=0.05). Results We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 µg/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 µg/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 µg/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion We conclude that FN at 10 µg/mL concentration exerted the most intense bioactive effects on hAPCs.
DOI: 10.6084/m9.figshare.19905160.v1
title: Bioactivity effects of extracellular matrix proteins on apical papilla cells

description: Abstract Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology Different concentrations (1, 5, and 10 µg/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5×103 cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; α=0.05). Results We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 µg/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 µg/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 µg/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion We conclude that FN at 10 µg/mL concentration exerted the most intense bioactive effects on hAPCs.