cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14740690.v1
title: The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China

description: ST59 is the predominant pathotype of community-associated methicillin-resistant <i>Staphylococcus aureus</i> (CA-MRSA) in China. As a variant of ST59, there is relatively little known about the detailed information of ST338. To address this issue, here, we described thirteen ST338 CA-MRSA strains isolated from severe bloodstream infection cases, and focused on their epidemiology, genetic features and virulence potential. Phylogenetic analysis showed the earliest isolated strain of this study is likely a predecessor of recent ST338 lineage (after year of 2014). Furthermore, the phylogenetic reconstruction and time estimation suggested that ST338 evolved from ST59 in 1991. Notably, the carrying patten of virulence factors of all ST338 strains were similar, and the genomic islands νSaα, νSaγ and SaPI and the core virulence factors like <i>hla</i> and <i>psm</i> were detected in ST338 isolates. However, all ST338 isolates lacked some adhesion factors such as <i>clfA</i>, <i>clfB</i>, <i>eap</i>, <i>cna</i> and <i>icaD</i>. Additionally, among these ST338 strains, one PVL-negative ST338 isolate was detected. Experiment on mice nose and human alveolar epithelial cell showed that the nasal colonization ability of ST338 was weaker than that of CA-MRSA MW2. In a mouse bloodstream infection model and skin infection model, PVL+ and PVL− strains had the similar virulence, which was dependent on upregulation of toxin genes rather than the presence of mobile genetic elements such as ΦSa2 carrying PVL. Our findings provide important insight into the epidemiology and pathogenicity of the novel and highly virulent ST338-SCC<i>mec</i> Vb clone.
DOI: 10.6084/m9.figshare.10025189
title: Analysis on genetic diversity of 51 Grape germplasm resources

description: ABSTRACT: The objective of this study is to research the genetic diversity of the ‘ Zuijinxiang ’ grape and its mutant breeding F1 plants, we screened the excellent mutant plants with potential breeding value. 50 mutated single plants obtained from 137Cs-γ irradiated ‘Zuijinxiang’ grape seeds were used as research objects, and SCoT molecular marker technology was used for genetic diversity and variation analysis, and clustering research was carried out. The results showed that: (1) 36 SCoT primers produced abundant polymorphisms, and the amplification results showed obvious bright bands, and the amplification efficiency and polymorphism rate were 100%. (2) A total of 221 bands were amplified by 36 primers, of which 175 were rich in polymorphism, the average polymorphic percentage was 80.3%, and the average genetic similarity coefficient was 0.916. (3) The number of observed alleles (Na) ranged from 4 to 8, with an average of 6.1389; the number of effective alleles (Ne) ranged from 1.2772 to 5.6322 with an average of 3.5968; the desired heterozygosity (He) The range is from 0.2192 to 0.8344, the average is 0.6965; the observed heterozygosity (Ho) ranges from 0.1656 to 0.7808 with an average of 0.3035; the Nei’s gene diversity index (H) ranges from 0.2170 to 0.8224 with an average of 0.6863; Shannon-Wiener The index (I) ranges from 0.5186 to 1.8597 with an average of 1.4517. (4) UPGMA clustering of 51 materials showed that the test materials could be divided into three groups when the genetic distance was 0.856. The experiment shows that the genetic diversity of the ‘Zuijinxiang’ radiation variation germplasm resources is rich. In addition, SCoT molecular marker technology can distinguish the materials with close genetic distance, and can be used for early identification techniques of grape mutant materials. This study provides a theoretical basis for the development of excellent mutant germplasm of ‘Zuijinxiang’ grapes.
DOI: 10.6084/m9.figshare.10025189.v1
title: Analysis on genetic diversity of 51 Grape germplasm resources

description: ABSTRACT: The objective of this study is to research the genetic diversity of the ‘ Zuijinxiang ’ grape and its mutant breeding F1 plants, we screened the excellent mutant plants with potential breeding value. 50 mutated single plants obtained from 137Cs-γ irradiated ‘Zuijinxiang’ grape seeds were used as research objects, and SCoT molecular marker technology was used for genetic diversity and variation analysis, and clustering research was carried out. The results showed that: (1) 36 SCoT primers produced abundant polymorphisms, and the amplification results showed obvious bright bands, and the amplification efficiency and polymorphism rate were 100%. (2) A total of 221 bands were amplified by 36 primers, of which 175 were rich in polymorphism, the average polymorphic percentage was 80.3%, and the average genetic similarity coefficient was 0.916. (3) The number of observed alleles (Na) ranged from 4 to 8, with an average of 6.1389; the number of effective alleles (Ne) ranged from 1.2772 to 5.6322 with an average of 3.5968; the desired heterozygosity (He) The range is from 0.2192 to 0.8344, the average is 0.6965; the observed heterozygosity (Ho) ranges from 0.1656 to 0.7808 with an average of 0.3035; the Nei’s gene diversity index (H) ranges from 0.2170 to 0.8224 with an average of 0.6863; Shannon-Wiener The index (I) ranges from 0.5186 to 1.8597 with an average of 1.4517. (4) UPGMA clustering of 51 materials showed that the test materials could be divided into three groups when the genetic distance was 0.856. The experiment shows that the genetic diversity of the ‘Zuijinxiang’ radiation variation germplasm resources is rich. In addition, SCoT molecular marker technology can distinguish the materials with close genetic distance, and can be used for early identification techniques of grape mutant materials. This study provides a theoretical basis for the development of excellent mutant germplasm of ‘Zuijinxiang’ grapes.
DOI: 10.24403/jp.1333624
title: Das Herbarium Haussknecht - Geschichte und Gegenwart / Friedrich Karl Meyer
DOI: 10.6084/m9.figshare.11598081
title: MOESM2 of Does Bangkok have a central role in the dengue dynamics of Thailand?

description: Additional file 2: Table S1. The names of the 716 districts included in this study and the provinces which these districts belong to.
DOI: 10.6084/m9.figshare.11598081.v1
title: MOESM2 of Does Bangkok have a central role in the dengue dynamics of Thailand?

description: Additional file 2: Table S1. The names of the 716 districts included in this study and the provinces which these districts belong to.
DOI: 10.6084/m9.figshare.10025240
title: U-Pb SHRIMP dating of basement rocks of the Iriri-Xingu domain, Central Amazonian province, Amazonian craton, Brazil

description: Abstract The Iriri-Xingu domain, located in the central part of the Amazonian craton, consists of extensive occurrences of Paleoproterozoic volcanic rocks and granites with published ages of ca. 1990 to 1840 Ma, which show a strong crustal contribution for their magmas. Exposures of basement rocks are small and rare. Samples from two areas were dated in this work by U-Pb SHRIMP in zircon. In the northern Maribel area, a high-grade pelitic paragneiss presented an age of 2160 ± 8 Ma and the leucosome of a migmatitic orthogneiss, probably a diatexite, crystallized at 2149 ± 20 Ma. These Rhyacian ages suggest that it is part of the Bacajá domain, related to the Trans-Amazonian cycle. The Morro Grande area occurs in the central part of Iriri-Xingu domain, where a high-grade muscovite gneiss with a protolith of 2120-2180 Ma is dated at 1982 ± 7 Ma, and the leucosome of a migmatitic orthogneiss is crystallized at 1979 ± 8 Ma. These are the first records of high-grade metamorphism at ca. 1980 Ma in the Central Brazil shield and may be related to the generation of this Orosirian felsic magmatism, which is widespread throughout the nearby Ventuari-Tapajós province.
DOI: 10.6084/m9.figshare.10025240.v1
title: U-Pb SHRIMP dating of basement rocks of the Iriri-Xingu domain, Central Amazonian province, Amazonian craton, Brazil

description: Abstract The Iriri-Xingu domain, located in the central part of the Amazonian craton, consists of extensive occurrences of Paleoproterozoic volcanic rocks and granites with published ages of ca. 1990 to 1840 Ma, which show a strong crustal contribution for their magmas. Exposures of basement rocks are small and rare. Samples from two areas were dated in this work by U-Pb SHRIMP in zircon. In the northern Maribel area, a high-grade pelitic paragneiss presented an age of 2160 ± 8 Ma and the leucosome of a migmatitic orthogneiss, probably a diatexite, crystallized at 2149 ± 20 Ma. These Rhyacian ages suggest that it is part of the Bacajá domain, related to the Trans-Amazonian cycle. The Morro Grande area occurs in the central part of Iriri-Xingu domain, where a high-grade muscovite gneiss with a protolith of 2120-2180 Ma is dated at 1982 ± 7 Ma, and the leucosome of a migmatitic orthogneiss is crystallized at 1979 ± 8 Ma. These are the first records of high-grade metamorphism at ca. 1980 Ma in the Central Brazil shield and may be related to the generation of this Orosirian felsic magmatism, which is widespread throughout the nearby Ventuari-Tapajós province.
DOI: 10.6084/m9.figshare.24895308
title: Visual opsin multiple sequence alignment file and output files from IQ-TREE

description: Multiple sequence alignment and phylogenetic analysis of visual opsins and their close relatives in vertebrates.Alignment was created using ClustalO with standard settings. The phylogenetic tree was created using IQ-TREE (v. 1.6.12) using the following settings: -t BIONJ -nt 8 -quiet -keep-ident -bb 10000 -alrt 10000 -m TEST -bnni.
DOI: 10.6084/m9.figshare.24895317
title: GNAT multiple sequence alignment and output files from IQ-TREE

description: Multiple sequence alignment and phylogenetic analysis of GNAT sequences from vertebrates.Alignment was created using ClustalO with standard settings. The phylogenetic tree was created using IQ-TREE (v. 1.6.12) using the following settings: -t BIONJ -nt 8 -quiet -keep-ident -bb 10000 -alrt 10000 -m TEST -bnni.
DOI: 10.6084/m9.figshare.24895515
title: Concatenated multiple sequence alignments of flanking genes to the visual opsins and output files from IQ-TREE

description: Multiple sequence alignment and phylogenetic analysis of concatenated flanking gene alignments of visual opsins in lampreys.Alignment was created using ClustalO with standard settings. The phylogenetic tree was created using IQ-TREE (v. 1.6.12) using the following settings: -t BIONJ -nt 8 -quiet -keep-ident -bb 10000 -alrt 10000 -m TEST -bnni.
DOI: 10.24403/jp.1333625
title: Joachim Burs[ch]er, der bedeutendste Florist im Europa des 17. Jahrhunderts / Hans-Peter Fuchs-Eckert
DOI: 10.25345/c5nk36g62
title: MassIVE MSV000094029 - GNPS C48 E48 RLAC CRG SS SAmple
title: College Research Grant Project Sample RLAC C48 and E48 ESKAPE
DOI: 10.22000/1919
title: Polyacrylonitrile Coated SiO2 Nanoparticles: Coating Thickness Characterization via Size Exclusion Chromatography
DOI: 10.6084/m9.figshare.24181095
title: Additional file 2 of Nuclear receptor RXRα binds the precursor of miR-103 to inhibit its maturation

description: Additional file 2. The individual data values for Fig. 1a-d; Fig. 2b-d; Fig. 3b-d; Fig. 4a-e; Fig. 5a-d; Fig. 6a, c, and e; Fig. 7a and b.
DOI: 10.6084/m9.figshare.24181095.v1
title: Additional file 2 of Nuclear receptor RXRα binds the precursor of miR-103 to inhibit its maturation

description: Additional file 2. The individual data values for Fig. 1a-d; Fig. 2b-d; Fig. 3b-d; Fig. 4a-e; Fig. 5a-d; Fig. 6a, c, and e; Fig. 7a and b.
DOI: 10.25592/uhhfdm.13976
title: Illumina sequencing of BnGRP7 enriched phloem RNAs

description: The RNA binding protein glycine-rich RNA binding protein 7 (GRP7) was immobilized and incubated with total phloem RNA of Brassica napus. Bound RNA was eluted in three steps and the third elution as well as total phloem RNA used as input was sent for library preparation and poly-A enriched and small RNA sequencing with Novogene (Cambridge, UK). Here, you can find the raw sequencing data of this sequencing run. GRP7 elutions were sequenced in biological triplicates for poly-A library and in duplicates for small RNAs. Input RNA was sequenced once.
DOI: 10.25592/uhhfdm.13975
title: Illumina sequencing of BnGRP7 enriched phloem RNAs

description: The RNA binding protein glycine-rich RNA binding protein 7 (GRP7) was immobilized and incubated with total phloem RNA of Brassica napus. Bound RNA was eluted in three steps and the third elution as well as total phloem RNA used as input was sent for library preparation and poly-A enriched and small RNA sequencing with Novogene (Cambridge, UK). Here, you can find the raw sequencing data of this sequencing run. GRP7 elutions were sequenced in biological triplicates for poly-A library and in duplicates for small RNAs. Input RNA was sequenced once.
DOI: 10.6084/m9.figshare.22604836
title: Additional file 1 of Identification of biomarkers predictive of metastasis development in early-stage colorectal cancer using network-based regularization

description: Additional file 1. Performance results and comparison of classifiers.
DOI: 10.6084/m9.figshare.22604836.v1
title: Additional file 1 of Identification of biomarkers predictive of metastasis development in early-stage colorectal cancer using network-based regularization

description: Additional file 1. Performance results and comparison of classifiers.
DOI: 10.24403/jp.1333626
title: John Sibthorp und John Hawkins auf den ionischen Inseln / H. Walter Lack
DOI: 10.25919/eg48-sz07
title: Parkes observations for project P456 semester 2008APRS_01

description: The Parkes Pulsar Timing Array (PPTA) project has three primary goals: (a) detection of gravitational waves from astronomical sources, (b) establishment of a pulsar timescale, and (c) improvement of our understanding of Solar-system dynamics. There are many secondary goals, some astrophysical and some instrumental/technique oriented. Achievement of these ambitious primary goals requires frequent observations of at least 20 MSPs at two or preferably three widely spaced frequencies over several years. We wish to continue observing the PPTA sample at intervals of 2-3 weeks using both the 10/50cm and Multibeam receivers. The digital filterbanks (PDFB3, PDFB4) and the baseband systems (CPSR2; APSR) are used for data recording. With the new instruments and development of an efficient pipeline processing system, we have achieved the world's best pulsar timing precision, with three pulsars having rms residuals of less than or about 100 ns and more than half less than 1 microsecond. We are collaborating with the European and North American pulsar timing array groups (EPTA and NANOGrav, respectively) to obtain more frequent observations and a larger pulsar sample. Because of the high sensitivity and wide bandwidths required, RFI mitigation is an important part of the project.
DOI: 10.24403/jp.1333628
title: Rapid isolation of total cell DNA from milligram plant tissue / Frank H. Hellwig, Martin Nolte, Jörg Ochsmann und Volker Wissemann
DOI: 10.6084/m9.figshare.14740689
title: Germline IGHV3-53-encoded RBD-targeting neutralizing antibodies are commonly present in the antibody repertoires of COVID-19 patients

description: Monoclonal antibodies (mAbs) encoded by IGHV3-53 (VH3-53) targeting the spike receptor-binding domain (RBD) have been isolated from different COVID-19 patients. However, the existence and prevalence of shared VH3-53-encoded antibodies in the antibody repertoires is not clear. Using antibody repertoire sequencing, we found that the usage of VH3-53 increased after SARS-CoV-2 infection. A highly shared VH3-53-J6 clonotype was identified in 9 out of 13 COVID-19 patients. This clonotype was derived from convergent gene rearrangements with few somatic hypermutations and was evolutionary conserved. We synthesized 34 repertoire-deduced novel VH3-53-J6 heavy chains and paired with a common IGKV1-9 light chain to produce recombinant mAbs. Most of these recombinant mAbs (23/34) possess RBD binding and virus-neutralizing activities, and recognize ACE2 binding site via the same molecular interface. Our computational analysis, validated by laboratory experiments, revealed that VH3-53 antibodies targeting RBD are commonly present in COVID-19 patients’ antibody repertoires, indicating many people have germline-like precursor sequences to rapidly generate SARS-CoV-2 neutralizing antibodies. Moreover, antigen-specific mAbs can be digitally obtained through antibody repertoire sequencing and computational analysis.
DOI: 10.17028/rd.lboro.25160099.v1
title: Supplementary information files for Impact of unhealthy food and beverage consumption on children’s risk of dental caries: a systematic review

description: © the Authors, CC-BY 4.0Supplementary files for article Impact of unhealthy food and beverage consumption on children’s risk of dental caries: a systematic reviewContext The impact of unhealthy foods and beverages, namely those high in sugar, salt, and saturated or trans fats, has been studied extensively in relation to weight, body composition, and noncommunicable diseases, but less so in relation to the risk of dental caries. Few previous reviews have examined the evidence from all countries globally. ObjectiveA systematic review was conducted to assess the impact of unhealthy food and beverage consumption on the risk of dental caries in children aged ≤10 years, commissioned by the World Health Organization to inform updated complementary feeding recommendations. Data Sources Systematic searches were conducted in the PubMed, Cochrane, and Embase databases for articles meeting the inclusion criteria dating from January 1971 to March 2022; supplementary searches were undertaken for articles from that period to June 2022. Data Extraction Unhealthy foods and beverages were identified using nutrient- and food-based approaches. Risk of bias was assessed using the Risk Of Bias In Non-randomized Studies of Interventions (ROBINS-I). Data Analysis A total of 30 023 unique citations were screened, yielding 37 studies for inclusion. Studies were conducted in high-income (n = 23 [62.2%]) or middle-income countries (n = 14 [37.8%]). Evidence synthesis was performed narratively, stratified by age (0 years to &lt;2 years, 2 years to &lt;5 years, and 5 years to ≤10 years) and exposure (unhealthy foods and unhealthy beverages). The heterogeneity of the exposures and comparators across studies was high. Almost all studies (n = 34) reported positive associations between the consumption of sugar-sweetened beverages or foods high in free sugars and dental caries. However, 67.6% of studies were assessed as having serious risk of bias. Conclusion The evidence indicates that the consumption of unhealthy food and beverages in children ≤10 years appears to increase the risk of dental caries. Further longitudinal studies with high-quality dietary assessments, including studies in low-income countries and children aged &gt;5 years at baseline, are recommended in order to build a more robust evidence base for use in the development of policy recommendations.
DOI: 10.6084/m9.figshare.22601660
title: Additional file 1 of Recovery from diabetes mellitus in primary aldosteronism patients after adrenalectomy

description: Additional file 1: Table 1. The drugs used to treat diabetes mellitus before and after adrenalectomy.
DOI: 10.25919/b6ae-xe61
title: Parkes observations for project P456 semester 2008OCTS_01

description: The Parkes Pulsar Timing Array (PPTA) project has three primary goals: (a) detection of gravitational waves from astronomical sources, (b) establishment of a pulsar timescale, and (c) improvement of our understanding of Solar-system dynamics. There are many secondary goals, some astrophysical and some instrumental/technique oriented. Achievement of these ambitious primary goals requires frequent observations of at least 20 MSPs at two or preferably three widely spaced frequencies over several years. We wish to continue observing the PPTA sample at intervals of 2-3 weeks using both the 10/50cm and Multibeam receivers. The digital filterbanks (PDFB3, PDFB4) and the baseband systems (CPSR2; APSR) are used for data recording. With the new instruments and development of an efficient pipeline processing system, we have achieved the world's best pulsar timing precision, with three pulsars having rms residuals of less than or about 100 ns and more than half less than 1 microsecond. We are collaborating with the European and North American pulsar timing array groups (EPTA and NANOGrav, respectively) to obtain more frequent observations and a larger pulsar sample. Because of the high sensitivity and wide bandwidths required, RFI mitigation is an important part of the project.
DOI: 10.6084/m9.figshare.23650163.v1
title: Additional file 2 of Astrocyte-derived SerpinA3N promotes neuroinflammation and epileptic seizures by activating the NF-κB signaling pathway in mice with temporal lobe epilepsy

description: Additional file 2: Table S1. Differential expression genes of hippocampus in temporal lobe epilepsy mice.
DOI: 10.24403/jp.1333629
title: Rosa ˣmangii, eine neue intersektionelle Hybride charakterisiert durch morphologische und genetische Untersuchungen / Annemarie Eigner und Volker Wissemann
DOI: 10.24403/jp.1333631
title: Felsmispeln (Cotoneaster sect. Cotoneaster) in Europa I / Tamara Krügel
DOI: 10.6084/m9.figshare.22604971.v1
title: Additional file 1 of GhAAO2 was observed responding to NaHCO3 stress in cotton compared to AAO family genes

description: Additional file 1.
DOI: 10.6084/m9.figshare.11598426
title: MOESM2 of Genetic architecture of quantitative traits in beef cattle revealed by genome wide association studies of imputed whole genome sequence variants: I: feed efficiency and component traits

description: Additional file 2. This file contains information of all suggestive significant SNPs/INDELs at P-value &lt; 0.005 based on the 7.8 M sequence variant GWAS for RFI, DMI, ADG, and MWT.
DOI: 10.6084/m9.figshare.11598426.v1
title: MOESM2 of Genetic architecture of quantitative traits in beef cattle revealed by genome wide association studies of imputed whole genome sequence variants: I: feed efficiency and component traits

description: Additional file 2. This file contains information of all suggestive significant SNPs/INDELs at P-value &lt; 0.005 based on the 7.8 M sequence variant GWAS for RFI, DMI, ADG, and MWT.
DOI: 10.24403/jp.1333632
title: Vervollständigung und Bestätigung der Chromosomenzahlen ausgewählter mitteleuropäischer Orobanche-Sippen / Focke Albers und Jürgen Pusch
DOI: 10.24403/jp.1333633
title: Chromosomenzahlen einiger europäischer Centaurea-Sippen (Asteraceae) / Jörg Ochsmann
DOI: 10.24403/jp.1333635
title: Validierung zweier Namen in Festuca L. für die Flora von Thüringen / Jochen Müller
DOI: 10.24403/jp.1333639
title: Zur Situation der Gerstensegge (Carex hordeistichos VILL.) in Thüringen / H. Korsch
DOI: 10.6084/m9.figshare.22604950
title: Additional file 3 of Uptight responses between clenching and forearm raising with factors of visual feedback and maintenance effort in healthy young women: An experimental study on factorial design

description: Additional file 3. Raw data of SEMG.
DOI: 10.6084/m9.figshare.22604947.v1
title: Additional file 2 of Uptight responses between clenching and forearm raising with factors of visual feedback and maintenance effort in healthy young women: An experimental study on factorial design

description: Additional file 2. Raw data of  VAS.
DOI: 10.6084/m9.figshare.22604947
title: Additional file 2 of Uptight responses between clenching and forearm raising with factors of visual feedback and maintenance effort in healthy young women: An experimental study on factorial design

description: Additional file 2. Raw data of  VAS.