cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7513106.v1
title: CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION

description: Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P&lt; 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values close to those found for motility (P&gt; 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology.
DOI: 10.6084/m9.figshare.7507676
title: CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR β MERCAPTOETHANOL

description: Cryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and β-mercaptoethanol (BM) was evaluated. After vitrification, oocytes were maturated in: TCM-199 (control); BM (24h TCM-199+100µM BM); LF (6h in LF+18h TCM-199), and LF+BM (6h LF+18h TCM-199+100µM BM). There was not difference (p&gt;0.05) in blastocysts rate in TCM (6.4%), BM (4.0%) and LF (3.4%) treatments. The hatching rates and cell density of hatched embryos did not differ (p&gt;0.05) among treatments. In Experiment 2, hatched blastocysts (Bx) obtained in D7 or D8 were vitrified and evaluated according to its expanding and hatching rates. The expanding rate was similar (p&gt;0.05), being observed a distinct pathway in hatching rate in D7 and D8 Bx. Higher hatching rate was observed in D7 Bx from control (TCM-54.2%) compared to BM (40.32%) and LF+BM (33.89%) treatments. The D8 Bx showed lower hatching rate in control (TCM-199) compared with D7 Bx. In BM, LF and LF+BM treatments, the hatching rate was similar for D7 or D8 embryos. Maturation with addition of LF and/or BM does not increase the oocyte or IVP embryo cryotolerance. Expanded blastocysts (D7) have higher cryotolerance and show a distinct pathway when added with LF or BM, in comparison with D8 embryos.
DOI: 10.6084/m9.figshare.7507676.v1
title: CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR β MERCAPTOETHANOL

description: Cryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and β-mercaptoethanol (BM) was evaluated. After vitrification, oocytes were maturated in: TCM-199 (control); BM (24h TCM-199+100µM BM); LF (6h in LF+18h TCM-199), and LF+BM (6h LF+18h TCM-199+100µM BM). There was not difference (p&gt;0.05) in blastocysts rate in TCM (6.4%), BM (4.0%) and LF (3.4%) treatments. The hatching rates and cell density of hatched embryos did not differ (p&gt;0.05) among treatments. In Experiment 2, hatched blastocysts (Bx) obtained in D7 or D8 were vitrified and evaluated according to its expanding and hatching rates. The expanding rate was similar (p&gt;0.05), being observed a distinct pathway in hatching rate in D7 and D8 Bx. Higher hatching rate was observed in D7 Bx from control (TCM-54.2%) compared to BM (40.32%) and LF+BM (33.89%) treatments. The D8 Bx showed lower hatching rate in control (TCM-199) compared with D7 Bx. In BM, LF and LF+BM treatments, the hatching rate was similar for D7 or D8 embryos. Maturation with addition of LF and/or BM does not increase the oocyte or IVP embryo cryotolerance. Expanded blastocysts (D7) have higher cryotolerance and show a distinct pathway when added with LF or BM, in comparison with D8 embryos.
DOI: 10.6084/m9.figshare.7509707.v1
title: SERUM LIPID PROFILE OF SPAYED AND NON-SPAYED FEMALE DOGS ASSOCIATED WITH THE BODY CONDITION SCORE

description: Neutering females may be associated to weight gain in dogs. In order to diagnose possible alterations, such as diabetes and hyperlipidemias, laboratory assessment of carbohydrate and lipid metabolism is important. The aim of this study was to verify glycemia and serum lipid profile in non-spayed and spayed female dogs, and associate these results with the body condition score. Thirty-two spayed (n=16) and non-spayed (n=16) female dogs had their blood collected for biochemical analysis, and were classified as to their body condition score (BCS). Nine had normal BCS, 10 were overweight, and 13 were obese. Little difference was observed among spayed and non-spayed dogs. Glycemia of spayed canine females is higher than that of non-spayed dogs, regardless of the body condition score. Likewise, total cholesterol levels are higher in non-spayed females. The HDL is higher in non-spayed obese dogs than in spayed dogs with the same body condition, this parameter did not differ in other weight groups. Contrary to what happens in post-menopausal women, spaying dogs does not seem to have such a strong effect on the metabolic parameters assessed. This study revealed unique results, where neither spaying nor weight group had a strong association with metabolic alterations.
DOI: 10.6084/m9.figshare.7509707
title: SERUM LIPID PROFILE OF SPAYED AND NON-SPAYED FEMALE DOGS ASSOCIATED WITH THE BODY CONDITION SCORE

description: Neutering females may be associated to weight gain in dogs. In order to diagnose possible alterations, such as diabetes and hyperlipidemias, laboratory assessment of carbohydrate and lipid metabolism is important. The aim of this study was to verify glycemia and serum lipid profile in non-spayed and spayed female dogs, and associate these results with the body condition score. Thirty-two spayed (n=16) and non-spayed (n=16) female dogs had their blood collected for biochemical analysis, and were classified as to their body condition score (BCS). Nine had normal BCS, 10 were overweight, and 13 were obese. Little difference was observed among spayed and non-spayed dogs. Glycemia of spayed canine females is higher than that of non-spayed dogs, regardless of the body condition score. Likewise, total cholesterol levels are higher in non-spayed females. The HDL is higher in non-spayed obese dogs than in spayed dogs with the same body condition, this parameter did not differ in other weight groups. Contrary to what happens in post-menopausal women, spaying dogs does not seem to have such a strong effect on the metabolic parameters assessed. This study revealed unique results, where neither spaying nor weight group had a strong association with metabolic alterations.
DOI: 10.6084/m9.figshare.7514606
title: ORIGIN AND ANTIMERIC DISTRIBUTION OF THE FEMORAL NERVES IN NEWBORNS GOATS OF THE SAANEN BREED

description: The femoral nerves were studied in newborn goats of Saanen breed (22 males and 11 females) that were collected after natural death and fixed with 10% formaldehyde solution. In males the femoral nerve arose from the ventral spinal branches of L4 and L5 in eight animals (36%); in six animals (27%) it arose from the ventral spinal branches of L5 and L6; in five animals (23%) it arose from the ventral spinal branches of L5; in two animals (9%) it arose from the ventral spinal branches of L4, L5 and L6 and in one animal (5%) it arose from the ventral spinal branches of L5 and L6 and the ventral spinal branches of S1. In females the femoral nerve arose from the ventral spinal branches of L4 and L5 in seven animals (64%); in three animals (27%) it arose from the ventral spinal branches of L5 and L6 and in one animal (9%) it arose from the ventral spinal branches of L4, L5 and L6. In all animals, the femoral nerves were distributed in different branches to the major and minor psoas, femoris quadriceps, sartorius and pectinius muscles.
DOI: 10.6084/m9.figshare.7514606.v1
title: ORIGIN AND ANTIMERIC DISTRIBUTION OF THE FEMORAL NERVES IN NEWBORNS GOATS OF THE SAANEN BREED

description: The femoral nerves were studied in newborn goats of Saanen breed (22 males and 11 females) that were collected after natural death and fixed with 10% formaldehyde solution. In males the femoral nerve arose from the ventral spinal branches of L4 and L5 in eight animals (36%); in six animals (27%) it arose from the ventral spinal branches of L5 and L6; in five animals (23%) it arose from the ventral spinal branches of L5; in two animals (9%) it arose from the ventral spinal branches of L4, L5 and L6 and in one animal (5%) it arose from the ventral spinal branches of L5 and L6 and the ventral spinal branches of S1. In females the femoral nerve arose from the ventral spinal branches of L4 and L5 in seven animals (64%); in three animals (27%) it arose from the ventral spinal branches of L5 and L6 and in one animal (9%) it arose from the ventral spinal branches of L4, L5 and L6. In all animals, the femoral nerves were distributed in different branches to the major and minor psoas, femoris quadriceps, sartorius and pectinius muscles.
DOI: 10.6084/m9.figshare.7518323
title: COMPARISON BETWEEN THE LABORATORY AND PORTABLE METHODS IN THE GLYCEMIC ANALYSIS IN CATS WITH CENTRAL VENOUS AND CAPILLARY BLOOD SAMPLES

description: The objective of this study was to compare the efficacy of portable blood glucose measurement method in relation to the laboratory reference method, using central venous and capillary blood samples from 20 cats. The value obtained by the laboratory method using central venous blood sample was considered as the standard reference value. Other two values were obtained from the portable blood glucose meter, in which a sample from the same central venous blood and another one sample from capillary blood were used. For the 20 cats, the blood glucose results obtained by the laboratory method had an average of 80mg/dL; and the results found by the glucometer had an average of 84.1mg/dL with central venous blood and of 73.1mg/dL with capillary blood. The glycemic averages obtained by the glucometer with both blood samples compared to that achieved by the laboratory method resulted in variations below the maximum established (20%) by associations that regulate portable glucometers and by the organization for standardization, as well as the variation between the glycemic averages obtained by the glucometer using different blood samples. In the comparative analysis, there was no significant difference between the methods (p&lt;0.05). In conclusion, the portable glucometer is effective in measuring blood glucose in cats, both from central venous blood and capillary blood samples.
DOI: 10.6084/m9.figshare.7518323.v1
title: COMPARISON BETWEEN THE LABORATORY AND PORTABLE METHODS IN THE GLYCEMIC ANALYSIS IN CATS WITH CENTRAL VENOUS AND CAPILLARY BLOOD SAMPLES

description: The objective of this study was to compare the efficacy of portable blood glucose measurement method in relation to the laboratory reference method, using central venous and capillary blood samples from 20 cats. The value obtained by the laboratory method using central venous blood sample was considered as the standard reference value. Other two values were obtained from the portable blood glucose meter, in which a sample from the same central venous blood and another one sample from capillary blood were used. For the 20 cats, the blood glucose results obtained by the laboratory method had an average of 80mg/dL; and the results found by the glucometer had an average of 84.1mg/dL with central venous blood and of 73.1mg/dL with capillary blood. The glycemic averages obtained by the glucometer with both blood samples compared to that achieved by the laboratory method resulted in variations below the maximum established (20%) by associations that regulate portable glucometers and by the organization for standardization, as well as the variation between the glycemic averages obtained by the glucometer using different blood samples. In the comparative analysis, there was no significant difference between the methods (p&lt;0.05). In conclusion, the portable glucometer is effective in measuring blood glucose in cats, both from central venous blood and capillary blood samples.
DOI: 10.6084/m9.figshare.7513148
title: BONE AND MUSCULAR ANATOMY OF THE SCAPULAR CINGULUM AND ARM IN Tapirus terrestris (PERISSODACTYLA: TAPIRIDAE)

description: Tapirus terrestris (Linneaus, 1758) is a mammal found in South America and in almost all Brazilian biomes. The aim of this study was to describe the anatomy of bone and muscle of the scapular cingulum and arm of Tapirus terrestris and compare it with other species of mammals, especially equines. We used four animals donated to the Laboratory of Education and Research of Wild Animals of the Federal University of Uberlândia, after their death with no trauma. The bones were carefully analyzed, described and the muscles were dissected, analyzed and described in accordance with the usual techniques of gross anatomy. The skeleton of the scapular cingulum and arm of Tapirus terrestris is formed by scapula and humerus bones, the lateral muscles of the scapula are subclavian m., deltoid m. supraspinatus m. and infraspinatus, teres minor m., subscapularis m., teres major m., coracobrachialis m., shoulder joint m., biceps brachii m., brachial m. triceps, forearm tensor fasciae m., anconeus m. The muscular and bone standard found is similar to the horse (Equus caballus) T. terrestris has bone and muscle characteristics of a suitable animal to displacement and eventual swimming with obvious bone accidents and developed muscles .
DOI: 10.6084/m9.figshare.7513148.v1
title: BONE AND MUSCULAR ANATOMY OF THE SCAPULAR CINGULUM AND ARM IN Tapirus terrestris (PERISSODACTYLA: TAPIRIDAE)

description: Tapirus terrestris (Linneaus, 1758) is a mammal found in South America and in almost all Brazilian biomes. The aim of this study was to describe the anatomy of bone and muscle of the scapular cingulum and arm of Tapirus terrestris and compare it with other species of mammals, especially equines. We used four animals donated to the Laboratory of Education and Research of Wild Animals of the Federal University of Uberlândia, after their death with no trauma. The bones were carefully analyzed, described and the muscles were dissected, analyzed and described in accordance with the usual techniques of gross anatomy. The skeleton of the scapular cingulum and arm of Tapirus terrestris is formed by scapula and humerus bones, the lateral muscles of the scapula are subclavian m., deltoid m. supraspinatus m. and infraspinatus, teres minor m., subscapularis m., teres major m., coracobrachialis m., shoulder joint m., biceps brachii m., brachial m. triceps, forearm tensor fasciae m., anconeus m. The muscular and bone standard found is similar to the horse (Equus caballus) T. terrestris has bone and muscle characteristics of a suitable animal to displacement and eventual swimming with obvious bone accidents and developed muscles .
DOI: 10.6084/m9.figshare.7516703
title: USE OF DIFFERENT EXTENDERS TO CRYOPRESERVATION OF MANGALARGA MARCHADOR SPERM

description: AbstractThe techniques applied to animal reproduction such as artificial insemination, transfer and in vitro production of embryos, heat synchronization and induction, and gametes cryopreservation have been more utilized in veterinary practice each day. Nevertheless, some techniques have not achieved their full technical capacity within the equine reproduction field, such as semen cryopreservation. The aim of this study was to evaluate the characteristics of post-thawing semen (total motility, strength, plasmatic and acrosomal membrane integrity) of Mangalarga Marchador breed stallions, using three different extenders (Botucrio, FR5 and FR6). Ejaculates from five stallions were collected and the gel-free semen was diluted in a 1:1 dilution in skim milk extender, and centrifuged at 600 g for 10 minutes. After the centrifugation the supernatant was removed and sperm pellet was divided and re-suspended using three different extenders to a concentration of 200 x 10<sup>6</sup> cells/mL. The samples were packed into 0.5 mL straws, placed in a stainless steel support and kept inside the refrigerator (5 ºC) for one hour. Subsequently, these straws were kept at a height of 6 cm from liquid nitrogen for 15 minutes in an isotherm box and, after that, plunged into liquid nitrogen (-196 ºC) and stored in a liquid nitrogen holding tank. There were no differences in the parameters evaluated when extenders using mixed amides and glycerol (Botucrio and FR6) were used (P &gt; 0.05). All parameters evaluated were lower for the extender containing only glycerol (P&lt;0.05). The use of cryoprotectants (methylformamide and dimethylformamide) in association with glycerol concentrations around 1 to 2% is an alternative for semen cryopreservation of Mangalarga Marchador breed stallions.
DOI: 10.6084/m9.figshare.7516703.v1
title: USE OF DIFFERENT EXTENDERS TO CRYOPRESERVATION OF MANGALARGA MARCHADOR SPERM

description: AbstractThe techniques applied to animal reproduction such as artificial insemination, transfer and in vitro production of embryos, heat synchronization and induction, and gametes cryopreservation have been more utilized in veterinary practice each day. Nevertheless, some techniques have not achieved their full technical capacity within the equine reproduction field, such as semen cryopreservation. The aim of this study was to evaluate the characteristics of post-thawing semen (total motility, strength, plasmatic and acrosomal membrane integrity) of Mangalarga Marchador breed stallions, using three different extenders (Botucrio, FR5 and FR6). Ejaculates from five stallions were collected and the gel-free semen was diluted in a 1:1 dilution in skim milk extender, and centrifuged at 600 g for 10 minutes. After the centrifugation the supernatant was removed and sperm pellet was divided and re-suspended using three different extenders to a concentration of 200 x 10<sup>6</sup> cells/mL. The samples were packed into 0.5 mL straws, placed in a stainless steel support and kept inside the refrigerator (5 ºC) for one hour. Subsequently, these straws were kept at a height of 6 cm from liquid nitrogen for 15 minutes in an isotherm box and, after that, plunged into liquid nitrogen (-196 ºC) and stored in a liquid nitrogen holding tank. There were no differences in the parameters evaluated when extenders using mixed amides and glycerol (Botucrio and FR6) were used (P &gt; 0.05). All parameters evaluated were lower for the extender containing only glycerol (P&lt;0.05). The use of cryoprotectants (methylformamide and dimethylformamide) in association with glycerol concentrations around 1 to 2% is an alternative for semen cryopreservation of Mangalarga Marchador breed stallions.
DOI: 10.6084/m9.figshare.7510190
title: PONDERAL AND REPRODUCTIVE PERFORMANCE DURING RAIN SEASON OF NELLORE PRIMIPAROUS HEIFERS PREVIOUSLY SUPPLEMENTED WITH PROTEIN IN DRY SEASON

description: AbstractThe objective of this study was to evaluate, in rainy season, the effect of protein complementation during dry season on Nellore primiparous heifers. We used 39 primiparous heifers, with an average age of 24-36 months, reared on Brachiaria decumbens and Brachiaria Brizantha pasture, for 205days. The animals were weighed every 28 days, after fasting for 12 hours. The experimental design was a split plot, the two treatments were distributed in two installments, the six periods in subplots and the animals were the replications. The following variables were analyzed: weight, hip height, body condition score, average daily weight gain and average monthly growth, presenting the respective values of 318.5 kg, 142 cm, 3.24, 0.580 kg and 2.55 cm respectively for previously supplemented animals, and 309.6 kg, 141 cm, 3.23, 0.584 and 2.29 kg cm for non-supplemented animals. There were no significant differences between the two experimental groups. Pregnancy rate of supplemented animals was 57% and it did not differ from non-supplemented animals' rate (45%). Calving rate was 100% for both groups. In the rainy season, after supplementation, heifers that did not receive supplementation during the dry period of the previous year had similar reproductive and weight performance as the supplemented ones.
DOI: 10.6084/m9.figshare.7510190.v1
title: PONDERAL AND REPRODUCTIVE PERFORMANCE DURING RAIN SEASON OF NELLORE PRIMIPAROUS HEIFERS PREVIOUSLY SUPPLEMENTED WITH PROTEIN IN DRY SEASON

description: AbstractThe objective of this study was to evaluate, in rainy season, the effect of protein complementation during dry season on Nellore primiparous heifers. We used 39 primiparous heifers, with an average age of 24-36 months, reared on Brachiaria decumbens and Brachiaria Brizantha pasture, for 205days. The animals were weighed every 28 days, after fasting for 12 hours. The experimental design was a split plot, the two treatments were distributed in two installments, the six periods in subplots and the animals were the replications. The following variables were analyzed: weight, hip height, body condition score, average daily weight gain and average monthly growth, presenting the respective values of 318.5 kg, 142 cm, 3.24, 0.580 kg and 2.55 cm respectively for previously supplemented animals, and 309.6 kg, 141 cm, 3.23, 0.584 and 2.29 kg cm for non-supplemented animals. There were no significant differences between the two experimental groups. Pregnancy rate of supplemented animals was 57% and it did not differ from non-supplemented animals' rate (45%). Calving rate was 100% for both groups. In the rainy season, after supplementation, heifers that did not receive supplementation during the dry period of the previous year had similar reproductive and weight performance as the supplemented ones.
DOI: 10.6084/m9.figshare.7506317
title: HEMATOLOGICAL PROFILE OF SANTA INÊS EWES SUPPLEMENTED ON PASTURE AT THE LAST THIRD OF PREGNANCY AND POSTPARTUM

description: AbstractThe purpose of this study was to evaluate the influence of concentrate supplementation level on hematological profile of Santa Ines ewes, at the last third of pregnancy and postpartum, and of their lambs. We used eleven ewes at the last 75 days of pregnancy and the first 75 days of lactation and eight lambs born from these ewes, allotted in a completely randomized design and split-plot arrangement in time. Blood samples were collected every fourteen days. Neither the erythrocyte nor the leukocyte profile was affected by the different levels of supplementation (P&gt;0.05) and the blood components evaluated were within the reference range for adult sheep. The animal category affected (P&lt;0.05) erythrocytes, and the highest values (11.72 x 106/p.l) were observed in lambs. Lambs showed lower values (3073.3/µL) for neutrophils in comparison with ewes, during pregnancy (4957.6/µL) or postpartum (4067.3/µL); these values did not differ (P&gt;0.05). For the lymphocyte count, the values found in lambs (2858.8 /µL) were similar to those in pregnant ewes (2,982.0/µL); lactating ewes (4119.8/µL) showed the highest values. Concentrate supplementation (0.5% BW) at pre and postpartum does not alter the erythrocyte and leukocyte profile of Santa Inês sheep, with the largest differences between the two sheep categories and lambs.