DOI: 10.5061/dryad.120
title: Data from: The contributions of sex, genotype and age to transcriptional variance in Drosophila melanogaster

description: Here we present a statistically rigorous approach to quantifying
 microarray expression data that allows the relative effects of multiple
 classes of treatment to be compared and incorporates analytical methods
 that are common to quantitative genetics. From the magnitude of gene
 effects and contributions of variance components, we find that gene
 expression in adult flies is affected most strongly by sex, less so by
 genotype and only weakly by age (for 1- and 6-wk flies); in addition, sex
 x genotype interactions may be present for as much as 10% of the
 Drosophila transcriptome. This interpretation is compromised to some
 extent by statistical issues relating to power and experimental design.
 Nevertheless, we show that changes in expression as small as 1.2-fold can
 be highly significant. Genotypic contributions to transcriptional variance
 may be of a similar magnitude to those relating to some quantitative
 phenotypes and should be considered when assessing the significance of
 experimental treatments.
description: MFCS15a2 Data
 SetOriginal ScanAlyze
 fileMFCS15a2.DATRR Slide 11b_Cy3RRSlide11b.Cy3.TIFMFCS3b Data SetOriginal ScanAlyze fileMFCS3b.DATMFCS1a Data SetOriginal ScanAlyze fileMFCS1a.DATRR Slide 16b_Cy3RRSlide16b.Cy3.TifREAD ME for AgingOriginal ScanAlyze fileREAD ME for Aging.docMFCS6a Data SetOriginal ScanAlyze fileMFCS6a.DATMFCS5a Data SetOriginal ScanAlyze fileMFCS5a.DATMFCS9a Data SetOriginal ScanAlyze fileMFCS9a.DATMFCS7a Data SetOriginal ScanAlyze fileMFCS7a.DATMFCS4a Data SetOriginal ScanAlyze fileMFCS4a.DATMFCS11a Data SetOriginal ScanAlyze fileMFCS11a.DATMFCS4b Data SetOriginal ScanAlyze filesMFCS4b.DATUMFOS data setGene Expression Profiling Results; this is an excel file with all of the extracted raw fluorescence intensities for Jin et al at the top, log2 at the bottomUMFOS.xlsRR Slide 10b_Cy5RRSlide10b.Cy5.TIFSGA sig Data SetGene Expression Profiling ResultsSGAsig.xlsMFCS10b Data SetOriginal ScanAlyze fileMFCS10b.DATMFCS15b2 Data SetOriginal ScanAlyze fileMFCS15b2.DATRR Slide 2a Data SetOriginal ScanAlyze fileRRslide2a.DATMFCS11b Data SetOriginal ScanAlyze fileMFCS11b.DATMFCS10a Data SetOriginal ScanAlyze fileMFCS10a.DATMFCS16a2 Data SetOriginal ScanAlyze fileMFCS16a2.DATRR Slide 16b_Cy5RRSlide16b.Cy5.TifMFCS9b Data SetOriginal ScanAlyze fileMFCS9b.DATRR Slide 16a_Cy3RRSlide16a.Cy3.TifMFCS5b Data SetOriginal ScanAlyze fileMFCS5b.DATRR Slide 10b_Cy3RRSlide10b.Cy3.TIFRR Slide 11b_Cy5RRSlide11b.Cy5.TIFMFCS12a2 Data SetOriginal ScanAlyze fileMFCS12a2.DATMFCS3a Data SetOriginal ScanAlyze fileMFCS3a.DATTemplate Data SetThis is a pdf showing the location of the 48 12x8 grids used to collect the data. Note that there are only 8 clones in the last grid of each sector [12, 24, 36, and 28]Template.pdfSex Specific List Data SetGene Expression Profiling ResultsSexSpecificList.txtRR Slide 16a_Cy5RRSlide16a.Cy5.TifRR Slide 2b Data SetOriginal ScanAlyze fileRRslide2b.DATMFCS7b Data SetOriginal ScanAlyze fileMFCS7b.DATUMFOS text fileGene Expression Profiling Results; this file contains the normalized relative fluorescence intensities used in the gene-specific models.UMFOS.txtMFCS6b Data SetOriginal ScanAlyze fileMFCS6b.DATMFCS1b Data SetOriginal ScanAlyze fileMFCS1b.DATClone LUT Data SetGene Expression Profiling Results; an Excel file listing the clones, their identity, and whether the sequence was reconfirmed after arrayingCloneLUT.xls