cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0zk5g14
title: CIL:39406, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: 11 micrographs of views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 5 food vacuoles and the edges of 2 more. The connection of the stalk with the cell body and the myonemes merging into the spasmoneme that passes into the contractile stalk are seen. The aboral end of the elongated macronucleus now appears. TEM taken on 4/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 2,150X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0th8kj9
title: CIL:39468, Drosophila. In Cell Image Library

description: Confocal micrograph of a Drosophila embryo stained for Pax3/7 (cyan), eve, horse radish peroxidase (red) and counterstained with DAPI (blue). Pax3/7 genes encode a class of transcription factors that play diverse developmental roles in most, if not all, metazoan taxa. Eve (even skipped gene) has a primary role in segmentation.
description: B0008300 Drosophila embryo. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); horseradish peroxidase
Processing history: unprocessed raw data
DOI: 10.6075/j0cc1001
title: CIL:39507, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The contractile vacuole pore is supported on its cytosolic side by a coil of microtubules and other microtubules extend from the pore to the CV membrane. The plasma membrane and CV membrane cover the bottom of the pore. Bacteria reside in the chamber. TEM taken on 2/14/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j03r0rqg
title: CIL:39465, lymphocyte, human immunodeficiency virus. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: detection of electrons
Source of contrast: differences in adsorption or binding of stain
Processing history: colorized
description: Colorized transmission electron micrograph of human immunodeficiency virus (HIV) particles (blue) budding from the surface of a T cell (a type of lymphocyte).The viruses replicate inside the cell with the different components gathering at the cell membrane to be assembled into new virus particles.
description: B0005750 HIV particles budding from a lymphocyte . Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j0dz07ms
title: CIL:39463, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Food vacuoles arise from the single membrane that lines the cytopharynx. Vesicles, similar to discoidal vesicles, attach to the cytopharyngeal microtubules (lamellar clumps) that arise from the dorsal ribbed wall of the peristome and that pass beyond the cytosome. The cytopharynx swells and a food vacuole balloons out through the ribs that are produced by the folded cytopharyngeal membrane and the attached lamellae. How, why and when a food vacuole pinches off needs further study. A myoneme equivalent to the cytostomal cord in Paramecium lies along the origins of the lamellae (called cytopharyngeal ribbons in Paramecium). TEM taken on 3/30/71 by R. Allen with Hitachi HU11A operating at 75kV. Mag. 9,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j00k27bf
title: CIL:39462, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The linkage complex consists of a spindle shaped mid piece bordered, in one plane, by several rails that have subunits that pass through the ER membrane. Thin filaments splay out from both tips of the mid piece and pass toward the surface of the pellicle. The molecular biology or biochemistry of these elements remains to be done. We can only guess that they are involved in calcium movement between the myoneme and the ER and thus in regulating myonemal contraction and relaxation. TEM taken on 4/15/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 24,000X. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0v40t3v
title: CIL:39466, Ascidiacea, embryonic cell. In Cell Image Library

description: B0008314 Ascidian embryo. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Early ascidian (sea squirt) embryos visualized by differential interface contrast (DIC) microscopy. Ascidians are used as a model for developmental research. Their simple embryonic development is rapid and can be easily manipulated. In addition, each embryo contains only a few cells therefore complex cellular processes can be studied while remaining part of an intact embryo. The embryos are also transparent, which is ideal for fluorescent microscopy thus allowing scientists to visualize different developmental stages. A higher magnification image is available as CIL 39471.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j0q81bxb
title: CIL:39461, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: V. convallaria preserved in collidine buffered fixative (not cacodylate) has a less dense cytoplasm in which oranelles are easier to recognize. The rough ER has vesiculated but this vesiculation enhances the identification of loci where ER remains bound to the myonemes. These loci on the ER are called ‚Äúlinkage complexes." These complexes are unique and have not been reported outside the peritrichs. Macronucleus with nucleolus and cytopharynx at its distal end. TEM taken on 3/30/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0rv0n16
title: CIL:39509, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: The spasmoneme contains membranous tubules that are known to store calcium (see Favard, P. and N. Carasso, J. Microscopie 3:671-696, 1964) and to be modified ER. The alveoli only partially surround the spasmoneme. TEM taken on 2/15/72 by R. Allen with Hitachi HU11A. Neg. 19,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0w958j0
title: CIL:39307, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Striated bands in a transverse ridge (in which kinetodesmal fibers are lacking) at high magnification. The longer band has seven stripes rather than five as in the shorter band. This suggests three regions of overlap rather than two. The filaments may have a set length so that for longer distances two sets of overlapping filaments may be needed to span the distance between the filaments arising from the epiplasm. TEM taken on 2/21/69 by R. Allen with Philips 300 operating at 60kV. Neg. 47,100X.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0xw4j55
title: CIL:39239, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: A glancing view of the cortex of Conchophthirus reveals details of cortical structure. A near serial section with CIL:39232. This includes the nature of the cell membrane and the alveolar membranes that reside immediately below the surface; fibrillar and microtubular structures that support this ciliated cell, and in the case of Conchophthirus, the cortical mitochondria that may represent a single mitochondrion that has finger-like extensions that lie immediately between the rows of basal bodies and are independent of more typical vesicular mitochondria that reside in the inner cytoplasm. The negative magnification is 14,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
DOI: 10.6075/j00z71zq
title: CIL:39255, Mus musculus, myoblast. In Cell Image Library

description: Mouse myoblasts stably transfected with GFP-PCNA to label replication foci and the 3D distribution of the GFP signal recorded over time using a confocal microscope. The time series illustrates the dynamic changes in relative strength of the GFP signal in individual replication factories.
description: Living C2C12 cells stably expressing GFP-PCNA were observed with a Leica TCS confocal microscope and z-series collected at 0.5 micrometer intervals over a 90 min period. 

See: Fig 6B in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT); charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: montage
Data qualification: Processed
DOI: 10.6075/j0bv7fx9
title: CIL:39249, Didinium nasutum, Paramecium sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: chemically fixed tissue; lyophilized specimen; Parducz fixative; Au/Pd metal sputter-coat
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: illumination by electrons; secondary_electron imaging
Parameter imaged: illumination by electrons; secondary electron generation; secondary_electron imaging
Source of contrast: detection of electrons
Visualization methods: shadowing and plating
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Didinium captures Paramecium. At midingestion and showing metachronous waves of cilia within the two characteristic ciliary girdles of Didinium nasutum. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 485X. Further details are available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270.
DOI: 10.6075/j0r49q2n
title: CIL:39241, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A transverse section of the cortex of Conchophthirus curtus reveals details of cortical structure. This includes the nature of the cell membrane and the alveolar membranes that reside immediately below the surface; fibrillar and microtubular structures that support this ciliated cell, and in the case of Conchophthirus, the cortical mitochondria that may represent a single mitochondrion that has finger-like extensions that lie immediately between the rows of basal bodies and are independent of more typical vesicular mitochondria that reside in the inner cytoplasm. Details of the basal bodies are revealed. The negative magnification is 15,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
DOI: 10.6075/j0hx1bbd
title: CIL:39252, Mus musculus, myoblast. In Cell Image Library

description: C2C12 cells stably expressing GFP-PCNA were fixed,stained for DNA ligase and DNA and optical sections acquired with a Leica TCS confocal microscope. 

See: Fig 2 in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
description: Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for the DNA methylase Dnmt1. DNA was localized using Hoechst staining. The image shows two different typical S-phase patterns displaying GFP-PCNA (left), Dnmt1 (center) and DNA (right). Dnmt1 co-localizes with PCNA at replication foci.
description: Preparation: formaldehyde fixed tissue; living tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT); charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Fluorescein (FITC); Hoechst 33258
Processing history: montage
Data qualification: Processed
DOI: 10.6075/j0zk5g0p
title: CIL:39247, Didinium nasutum, Paramecium sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Didinium captures Paramecium. After reeling in the prey with cyclosis tugging at the attached toxicysts the proboscis opens to engage the Paramecium. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 520X. Further details are available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270.
description: Preparation: chemically fixed tissue; lyophilized specimen; Parducz fixative; Au/Pd metal sputter-coat
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: illumination by electrons; secondary_electron imaging
Parameter imaged: illumination by electrons; secondary electron generation; secondary_electron imaging
Source of contrast: detection of electrons
Visualization methods: shadowing and plating
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation