cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.28622073
title: PTK6 drives HNRNPH1 phase separation to activate autophagy and suppress apoptosis in colorectal cancer

description: Macroautophagy/autophagy is the principal mechanism that mediates the delivery of various cellular cargoes to lysosomes for degradation and recycling, and has been reported to play a crucial role in colorectal cancer (CRC) pathogenesis and progression. Targeting autophagy may be a promising therapeutic strategy for CRC. However, the specific functions and potential mechanisms of autophagy in CRC remain unclear. In the present study, we discovered that PTK6 (protein tyrosine kinase 6) could activate autophagy and inhibit CRC apoptosis. PTK6 physically interacted with HNRNPH1 and mediated tyrosine phosphorylation at Y210 of HNRNPH1, which promoted the latter’s liquid-liquid phase separation (LLPS). Furthermore, LLPS of HNRNPH1 formed biomolecular condensates and triggered splicing-switching of the <i>NBR1</i> exon 10 inclusion transcript, thereby activating autophagy and suppressing apoptosis of CRC. Additionally, PDO and CDX models indicated that tilfrinib, an inhibitor targeting PTK6, could inhibit CRC growth. Overall, our findings reveal the novel PTK6-HNRNPH1-NBR1 regulatory autophagy axis and provide a potential therapy target for CRC. <b>Abbreviation</b>: 1,6HD: 1,6-hexanediol, CQ: chloroquine, CRC: colorectal cancer, DFS: disease-free survival, FRAP: fluorescence recovery afterphotobleaching, GSEA: Gene Set Enrichment Analysis, GTEx: Genotype-Tissue Expression, HNRNPH1: heterogeneous nuclearribonucleoprotein H1, IDRs: intrinsically disordered regions, IHC: immunohistochemical, KEGG: Kyoto Encyclopedia of Genes and Genomes,LLPS: liquid-liquid phase separation, NBR1: NBR1 autophagy cargoreceptor, OS: overall survival, PDO: patient-derivedorganoid, PTK6: protein tyrosine kinase 6, PTMs: post-translationalmodifications, SE: skipped exon, TCGA: The Cancer Genome Atlas, TEM: transmission electron microscopy, TMA: tissue microarray, TyrKc: tyrosine kinase catalytic.
DOI: 10.6084/m9.figshare.28622073.v1
title: PTK6 drives HNRNPH1 phase separation to activate autophagy and suppress apoptosis in colorectal cancer

description: Macroautophagy/autophagy is the principal mechanism that mediates the delivery of various cellular cargoes to lysosomes for degradation and recycling, and has been reported to play a crucial role in colorectal cancer (CRC) pathogenesis and progression. Targeting autophagy may be a promising therapeutic strategy for CRC. However, the specific functions and potential mechanisms of autophagy in CRC remain unclear. In the present study, we discovered that PTK6 (protein tyrosine kinase 6) could activate autophagy and inhibit CRC apoptosis. PTK6 physically interacted with HNRNPH1 and mediated tyrosine phosphorylation at Y210 of HNRNPH1, which promoted the latter’s liquid-liquid phase separation (LLPS). Furthermore, LLPS of HNRNPH1 formed biomolecular condensates and triggered splicing-switching of the <i>NBR1</i> exon 10 inclusion transcript, thereby activating autophagy and suppressing apoptosis of CRC. Additionally, PDO and CDX models indicated that tilfrinib, an inhibitor targeting PTK6, could inhibit CRC growth. Overall, our findings reveal the novel PTK6-HNRNPH1-NBR1 regulatory autophagy axis and provide a potential therapy target for CRC. <b>Abbreviation</b>: 1,6HD: 1,6-hexanediol, CQ: chloroquine, CRC: colorectal cancer, DFS: disease-free survival, FRAP: fluorescence recovery afterphotobleaching, GSEA: Gene Set Enrichment Analysis, GTEx: Genotype-Tissue Expression, HNRNPH1: heterogeneous nuclearribonucleoprotein H1, IDRs: intrinsically disordered regions, IHC: immunohistochemical, KEGG: Kyoto Encyclopedia of Genes and Genomes,LLPS: liquid-liquid phase separation, NBR1: NBR1 autophagy cargoreceptor, OS: overall survival, PDO: patient-derivedorganoid, PTK6: protein tyrosine kinase 6, PTMs: post-translationalmodifications, SE: skipped exon, TCGA: The Cancer Genome Atlas, TEM: transmission electron microscopy, TMA: tissue microarray, TyrKc: tyrosine kinase catalytic.
DOI: 10.6084/m9.figshare.28637321.v1
title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease

description: Microplastics (MPs) induce mitochondrial dysfunction and iron accumulation, contributing to mitochondrial macroautophagy/autophagy and ferroptosis, which has increased susceptibility to the exacerbation of chronic obstructive pulmonary disease (COPD); however, the underlying mechanism remains unclear. We demonstrated that MPs intensified inflammation in COPD by enhancing autophagy-dependent ferroptosis (ADF) in vitro and in vivo. In the lung tissues of patients with COPD, the concentrations of MPs, especially polystyrene microplastics (PS-MPs), were significantly higher than that of the control group, as detected by pyrolysis gas chromatography mass spectrometry (Py-GCMS), with increased iron accumulation. The exposure to PS-MPs, 2 μm in size, resulted in their being deposited in the lungs of COPD model mice detected by optical in vivo imaging, and observed in bronchial epithelial cells traced by GFP-labeled PS-MPs. There were mitochondrial impairments accompanied by mitochondrial reactive oxygen species (mito-ROS) overproduction and significantly increased levels of lysosome biogenesis and acidification in pDHBE cells with PS-MP stimulation, triggering occurrence of ferritinophagy and enhancing ADF in COPD, which triggered acute exacerbation of COPD (AECOPD). Reestablishing autophagy-dependent ferroptosis via mitochondria-specific ROS scavenging or ferroptosis inhibition alleviated excessive inflammation and ameliorated AECOPD induced by PS-MPs. Collectively, our data initially revealed that MPs exacerbate ferroptosis via mito-ROS-mediated autophagy in COPD, which sheds light on further hazard assessments of MPs on human respiratory health and potential therapeutic agents for patients with COPD.
DOI: 10.6084/m9.figshare.28605357
title: The alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy

description: The zoonotic transmission of coronaviruses continues to pose a considerable threat to humans. Swine acute diarrhea syndrome coronavirus (SADS-CoV), a bat coronavirus related to HKU2, causes severe economic losses in the pig industry and has the potential to trigger outbreaks in humans. However, our understanding of how SADS-CoV evades the host’s innate immunity remains limited, hindering effective responses to potential human outbreaks. In this study, we demonstrate that the SADS-CoV envelope protein (E) inhibits type I interferon (IFN-I) signaling by inducing the degradation of STAT2 via the macroautophagy/autophagy-lysosome pathway. Mechanistically, the E protein evades host innate immunity by promoting STAT2 degradation through autophagy, mediated by the NBR1 and OPTN receptors. Notably, ubiquitination of E protein is required for the autophagic degradation of STAT2. Additionally, lysine residue K61 of the E protein is crucial for its stable expression; however, it is not involved in its ubiquitination. In conclusion, our study reveals a novel mechanism by which the E protein disrupts IFN-I signaling by targeting STAT2 via autophagy, enhancing our understanding of SADS-CoV’s immune evasion strategies and providing potential drug targets for controlling viral infections. <b>Abbreviations</b>: 3-MA: 3-methyladenine; ATG: autophagy related; BafA1: bafilomycin A<sub>1</sub>; BSA: bovine serum albumin; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; CC: coiled-coil; CHX: cycloheximide; Co-IP: co-immunoprecipitation; DAPI: 4’,6-diamidino-2-phenylindole; DBD: DNA-binding domain; DMEM: Dulbecco’s Modified Eagle’s medium; DMSO: dimethyl sulfoxide; E, Envelope. FW: four-tryptophan; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HA: hemagglutinin; hpt: hours post-treatment; IF: indirect immunofluorescence; IFNB/IFN-β: interferon beta; IgG: immunoglobulin G; ISG: IFN-stimulated genes; ISRE: interferon-stimulated response element; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MOI: multiplicity of infection; NBR1: NBR1 autophagy cargo receptor; OPTN: optineurin; PBS: phosphate-buffered saline; PRRs: pattern recognition receptors; qPCR: quantitative polymerase chain reaction; SAR: selective autophagy receptor; SQSTM1/p62: sequestosome 1; STAT: signal transduction and activator of transcription; TBS-T: Tris-buffered saline with Tween 20; TCID50: 50% tissue culture infective dose; TOLLIP: toll interacting protein; Ub: ubiquitin; UBA: C-terminal ubiquitin-associated; VSV: vesicular stomatitis virus; WB: western blotting. WT: wild type.
DOI: 10.6084/m9.figshare.28605357.v2
title: The alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy

description: The zoonotic transmission of coronaviruses continues to pose a considerable threat to humans. Swine acute diarrhea syndrome coronavirus (SADS-CoV), a bat coronavirus related to HKU2, causes severe economic losses in the pig industry and has the potential to trigger outbreaks in humans. However, our understanding of how SADS-CoV evades the host’s innate immunity remains limited, hindering effective responses to potential human outbreaks. In this study, we demonstrate that the SADS-CoV envelope protein (E) inhibits type I interferon (IFN-I) signaling by inducing the degradation of STAT2 via the macroautophagy/autophagy-lysosome pathway. Mechanistically, the E protein evades host innate immunity by promoting STAT2 degradation through autophagy, mediated by the NBR1 and OPTN receptors. Notably, ubiquitination of E protein is required for the autophagic degradation of STAT2. Additionally, lysine residue K61 of the E protein is crucial for its stable expression; however, it is not involved in its ubiquitination. In conclusion, our study reveals a novel mechanism by which the E protein disrupts IFN-I signaling by targeting STAT2 via autophagy, enhancing our understanding of SADS-CoV’s immune evasion strategies and providing potential drug targets for controlling viral infections. <b>Abbreviations</b>: 3-MA: 3-methyladenine; ATG: autophagy related; BafA1: bafilomycin A<sub>1</sub>; BSA: bovine serum albumin; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; CC: coiled-coil; CHX: cycloheximide; Co-IP: co-immunoprecipitation; DAPI: 4’,6-diamidino-2-phenylindole; DBD: DNA-binding domain; DMEM: Dulbecco’s Modified Eagle’s medium; DMSO: dimethyl sulfoxide; E, Envelope. FW: four-tryptophan; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HA: hemagglutinin; hpt: hours post-treatment; IF: indirect immunofluorescence; IFNB/IFN-β: interferon beta; IgG: immunoglobulin G; ISG: IFN-stimulated genes; ISRE: interferon-stimulated response element; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MOI: multiplicity of infection; NBR1: NBR1 autophagy cargo receptor; OPTN: optineurin; PBS: phosphate-buffered saline; PRRs: pattern recognition receptors; qPCR: quantitative polymerase chain reaction; SAR: selective autophagy receptor; SQSTM1/p62: sequestosome 1; STAT: signal transduction and activator of transcription; TBS-T: Tris-buffered saline with Tween 20; TCID50: 50% tissue culture infective dose; TOLLIP: toll interacting protein; Ub: ubiquitin; UBA: C-terminal ubiquitin-associated; VSV: vesicular stomatitis virus; WB: western blotting. WT: wild type.
DOI: 10.6084/m9.figshare.28605357.v1
title: The Alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy

description: The zoonotic transmission of coronaviruses continues to pose a considerable threat to humans. Swine acute diarrhea syndrome coronavirus (SADS-CoV), a bat coronavirus related to HKU2, causes severe economic losses in the pig industry and has the potential to trigger outbreaks in humans. However, our understanding of how SADS-CoV evades the host’s innate immunity remains limited, hindering effective responses to potential human outbreaks. In this study, we demonstrate that the SADS-CoV envelope protein (E) inhibits type I interferon (IFN-I) signaling by inducing the degradation of STAT2 via the macroautophagy/autophagy-lysosome pathway. Mechanistically, the E protein evades host innate immunity by promoting STAT2 degradation through autophagy, mediated by the NBR1 and OPTN receptors. Notably, ubiquitination of E protein is required for the autophagic degradation of STAT2. Additionally, lysine residue K61 of the E protein is crucial for its stable expression; however, it is not involved in its ubiquitination. In conclusion, our study reveals a novel mechanism by which the E protein disrupts IFN-I signaling by targeting STAT2 via autophagy, enhancing our understanding of SADS-CoV’s immune evasion strategies and providing potential drug targets for controlling viral infections.
DOI: 10.15468/dl.uh432h
title: Occurrence Download

description: A dataset containing 1616 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Isachne arundinacea"
 ]
}

 The dataset includes 1616 records from 99 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0033329-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.b6tdjr
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Anemiaceae"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204128-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vgteke
title: Occurrence Download

description: A dataset containing 47 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Salviniaceae"
 ]
}

 The dataset includes 47 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204120-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5mpnck
title: Occurrence Download

description: A dataset containing 63 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Marsileaceae"
 ]
}

 The dataset includes 63 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204107-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7z8wd6
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Culcitaceae"
 ]
}

 The dataset includes 6 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204100-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.sdkw7m
title: Occurrence Download

description: A dataset containing 225 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Plagiogyriaceae"
 ]
}

 The dataset includes 225 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204093-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.57892/100-42
title: Impedance and compliance surfaces for twin adjacent foundations
DOI: 10.15468/dl.4tbz93
title: Occurrence Download

description: A dataset containing 254 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Dicksoniaceae"
 ]
}

 The dataset includes 254 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204069-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.r5ahyq
title: Occurrence Download

description: A dataset containing 155 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Cibotiaceae"
 ]
}

 The dataset includes 155 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204088-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yys9qd
title: Occurrence Download

description: A dataset containing 2152 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Cyatheaceae"
 ]
}

 The dataset includes 2152 records from 44 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204055-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.p27mkp
title: Occurrence Download

description: A dataset containing 100 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Saccolomataceae"
 ]
}

 The dataset includes 100 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0204043-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9v5ynp
title: Occurrence Download

description: A dataset containing 4972 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Thelypteridaceae"
 ]
}

 The dataset includes 4972 records from 55 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0210949-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dupsx5
title: Occurrence Download

description: A dataset containing 3969 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "TaxonKey is Asplenium L."
 ]
}

 The dataset includes 3969 records from 50 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0022324-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5517/cc5fnw3
title: CCDC 162095: Experimental Crystal Structure Determination

description: Related Article: M.I.Alcalde, A.J.Carty, Y.Chi, E.Delgado, B.Donnadieu, E.Hernandez, K.Dallmann, J.Sanchez-Nieves|2001|J.Chem.Soc.,Dalton Trans.||2502|doi:10.1039/b103202j
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc5fny5
title: CCDC 162097: Experimental Crystal Structure Determination

description: Related Article: M.I.Alcalde, A.J.Carty, Y.Chi, E.Delgado, B.Donnadieu, E.Hernandez, K.Dallmann, J.Sanchez-Nieves|2001|J.Chem.Soc.,Dalton Trans.||2502|doi:10.1039/b103202j
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc58tx4
title: CCDC 157446: Experimental Crystal Structure Determination

description: Related Article: C.Dieleman, S.Steyer, C.Jeunesse, D.Matt|2001|J.Chem.Soc.,Dalton Trans.||2508|doi:10.1039/b101369f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc5fnx4
title: CCDC 162096: Experimental Crystal Structure Determination

description: Related Article: M.I.Alcalde, A.J.Carty, Y.Chi, E.Delgado, B.Donnadieu, E.Hernandez, K.Dallmann, J.Sanchez-Nieves|2001|J.Chem.Soc.,Dalton Trans.||2502|doi:10.1039/b103202j
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc5f9jd
title: CCDC 161743: Experimental Crystal Structure Determination

description: Related Article: R.Stahler, W.Bensch|2001|J.Chem.Soc.,Dalton Trans.||2518|doi:10.1039/b102273n
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc5f9kf
title: CCDC 161744: Experimental Crystal Structure Determination

description: Related Article: R.Stahler, W.Bensch|2001|J.Chem.Soc.,Dalton Trans.||2518|doi:10.1039/b102273n
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc5kgqw
title: CCDC 165748: Experimental Crystal Structure Determination

description: Related Article: E.M.Barranco, O.Crespo, M.C.Gimeno, P.G.Jones, A.Laguna, C.Sarroca|2001|J.Chem.Soc.,Dalton Trans.||2523|doi:10.1039/b102636b
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc5kgrx
title: CCDC 165749: Experimental Crystal Structure Determination

description: Related Article: E.M.Barranco, O.Crespo, M.C.Gimeno, P.G.Jones, A.Laguna, C.Sarroca|2001|J.Chem.Soc.,Dalton Trans.||2523|doi:10.1039/b102636b
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.