cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5241277.v1
title: Erratum: Cerebral Amyloid Angiopathy, Blood-Brain Barrier Disruption and Amyloid Accumulation in SAMP8 Mice

description: Cerebrovascular dysfunction and β-amyloid peptide deposition on the walls of cerebral blood vessels might be an early event in the development of Alzheimer’s disease. Here we studied the time course of amyloid deposition in blood vessels and blood-brain barrier (BBB) disruption in the CA1 subzone of the hippocampus of SAMP8 mice and the association between these two variables. We also studied the association between the amyloid deposition in blood vessels and the recently described amyloid clusters in the parenchyma, as well as the association of these clusters with vessels in which the BBB is disrupted. SAMP8 mice showed greater amyloid deposition in blood vessels than age-matched ICR-CD1 control mice. Moreover, at 12 months of age the number of vessels with a disrupted BBB had increased in both strains, especially SAMP8 animals. At this age, all the vessels with amyloid deposition showed BBB disruption, but several capillaries with an altered BBB showed no amyloid on their walls. Moreover, amyloid clusters showed no spatial association with vessels with amyloid deposition, nor with vessels in which the BBB had been disrupted. Finally, we can conclude that vascular amyloid deposition seems to induce BBB alterations, but BBB disruption may also be due to other factors.
DOI: 10.6084/m9.figshare.5241280.v1
title: Erratum: A New Expectation-Maximization Statistical Test for Case-Control Association Studies Considering Rare Variants Obtained by High-Throughput Sequencing

description: Genome-wide association studies (GWAS) have been successful in identifying common genetic variation reproducibly associated with disease. However, most associated variants confer very small risk and after meta-analysis of large cohorts a large fraction of expected heritability still remains unexplained. A possible explanation is that rare variants currently undetected by GWAS with SNP arrays could contribute a large fraction of risk when present in cases. This concept has spurred great interest in exploring the role of rare variants in disease. As the cost of sequencing continue to plummet, it is becoming feasible to directly sequence case-control samples for testing disease association including rare variants. We have developed a test statistic that allows for association testing among cases and controls using data directly from sequencing reads. In addition, our method allows for random errors in reads. We determine the probability of a true genotype call based on the observed base pair reads using the expectation-maximization algorithm. We apply the <i>SumStat</i> procedure to obtain a single statistic for a group of multiple rare variant loci. We document the validity of our method through simulations. Our results suggest that our statistic maintains the correct type I error rate, even in the presence of differential misclassification for sequence reads, and that it has good power under a number of scenarios. Finally, our <i>SumStat</i> results show power at least as good as the maximum single locus results.
DOI: 10.6084/m9.figshare.5241283.v1
title: Erratum: Impact of Intravitreal Injection of Tissue Plasminogen Activator on Full-Field Electroretinogram in Patients with Macular Oedema Secondary to Retinal Vein Occlusion

description: <i>Purpose:</i> To evaluate the retinal toxicity of intravitreal tissue plasminogen activator (tPA) injection for branch and central retinal vein occlusion (BRVO and CRVO) using the electroretinogram (ERG). <i>Procedures:</i> Ten BRVO patients and 5 CRVO patients were enrolled. A complete examination including full-field ERG, visual acuity, central retinal thickness (CRT), and evaluation of systemic and ocular complications was performed before and after intravitreal tPA injection. <i>Results:</i> No significant differences were found in the amplitude or implicit time of any ERG component after tPA injection, and no systemic or ocular complication was observed. The improvement of visual acuity was significant at month 3 in the BRVO group (p &lt; 0.05) but not in the CRVO group. CRT significantly decreased over the course of 3 months in both groups (p &lt; 0.01). <i>Conclusion:</i> Intravitreal tPA injection seems to be a safe and effective treatment option for macular oedema caused by retinal vein occlusions.
DOI: 10.6084/m9.figshare.5241262
title: Erratum: Motor Cortex Stimulation for Trigeminal Neuropathic or Deafferentation Pain: An Institutional Case Series Experience

description: <i>Background:</i> Trigeminal neuropathy is a rare, devastating condition that can be intractable and resistant to treatment. When medical treatment fails, invasive options are limited. Motor cortex stimulation (MCS) is a relatively recent technique introduced to treat central neuropathic pain. The use of MCS to treat trigeminal neuropathic or deafferentation pain is not widespread and clinical data in the medical literature that demonstrate efficacy are limited. <i>Method:</i> We retrospectively reviewed patients with trigeminal neuropathic or trigeminal deafferentation pain who were treated at the Oregon Health & Science University between 2001 and 2008 by 1 neurosurgeon using MCS. <i>Results:</i> Eight of 11 patients (3 male, 8 female) underwent successful permanent implantation of an MCS system. All 8 patients reported initial satisfactory pain control. Three failed to experience continued pain control (6 months of follow-up). Five continued to experience long-term pain control (mean follow-up, 33 months). Average programming sessions were 2.2/year (all 8 patients) and 1.55/year (5 patients who sustained long-term pain control). Patients with anesthesia dolorosa or trigeminal deafferentation pain who had previously undergone ablative trigeminal procedures responded poorly to MCS. We encountered no perioperative complications. <i>Conclusion:</i> MCS is a safe and potentially effective therapy in certain patients with trigeminal neuropathy.
DOI: 10.6084/m9.figshare.5241265
title: Erratum: Chromosome Mapping of H1 Histone and 5S rRNA Gene Clusters in Three Species of <i>Astyanax </i>(Teleostei, Characiformes)

description: We report here on the physical mapping of the H1 histone genes (hisDNA) and the 5S ribosomal DNA (rDNA) in 3 Neotropical fish species of the genus <i>Astyanax</i><i>(A. altiparanae</i>,<i> A. bockmanni </i>and<i> A. fasciatus)</i> and the comparative analysis of the chromosomes bearing these genes. Nucleotide analyses by sequencing of both genes were also performed. The distribution of the H1 histone genes was more conserved than that of the rRNA genes, since these were always located in the pericentromeric regions of 2 chromosome pairs. 5S rDNA was found on one of the pairs that presented an H1 histone cluster; this seems to be a conserved chromosomal feature of the genus <i>Astyanax</i>. In addition, individuals of <i>A. bockmanni </i>and<i> A. fasciatus</i> showed clusters of 5S rDNA on 1 pair of acrocentric chromosomes, not found in <i>A. altiparanae</i>. The results obtained by chromosome mapping as well as by sequencing of both genes showed that <i>A.</i><i>bockmanni</i> is more closely related to <i>A. fasciatus</i> than to <i>A. altiparanae</i>. The results allow the characterization of cytogenetic markers for improved elucidation of the processes involved in karyotype differentiation of fish genomes.
DOI: 10.6084/m9.figshare.5241268
title: Erratum: The Relationship between Insight and Uncertainty in Obsessive-Compulsive Disorder

description: <i>Background:</i> The aim of this study was to investigate the relationship between the levels of insight and checking-related uncertainty in patients with obsessive-compulsive disorder (OCD). <i>Sampling and Methods:</i> Twenty OCD patients with checking compulsions and without current comorbidity were recruited. We used an experimental paradigm that gave subjects the opportunity to check during a decision-making task, thereby allowing for the calculation of a response time index (RTI) as the ‘uncertainty cost’ during decision-making. The level of insight was assessed with the Brown Assessment of Beliefs Scale (BABS). <i>Results:</i> Regression analyses indicated a significant positive correlation between RTI and BABS scores (r = 0.49). <i>Conclusions:</i> The level of insight is related to cognitive characteristics underlying OCD symptoms, in particular, checking-related uncertainty in checking OCD patients. <i>Study Limitations:</i> The absence of a comparison group and the low number of included patients are the main limitations of the present study.
DOI: 10.6084/m9.figshare.5241259
title: Erratum: Skeletal Muscle Metabolic Gene Expression Is Not Affected by Dichloroacetate-Mediated Modulation of Substrate Utilisation

description: <i>Aim:</i> This study investigated whether changing fuel use, by increasing pyruvate dehydrogenase complex (PDC) flux, independently of plasma substrate availability and insulin signalling, would alter metabolic gene expression. <i>Methods:</i> The PDC activator, dichloroacetate (DCA), was administered as an intravenous infusion in healthy male subjects at a rate of 50 mg kg<sup>–1</sup> min<sup>–1</sup>, for 90 min. Saline was infused as a control (CON) on a separate occasion in a randomised sequence. Muscle biopsies were taken from the vastus lateralis at 0 and 30 min into the infusion and 90 min after infusion. Gene expression was quantified using RT-qPCR, and immunoblotting was used to confirm that there were no changes in insulin signalling via the PI3K/Akt pathway. <i>Results:</i> Blood glucose concentrations fell during both trials but 3 h after the start of the infusion they were lower in DCA (p &lt; 0.05) than CON. Blood lactate concentrations also declined in both trials (p &lt; 0.01), however, this decrease was also more pronounced in DCA than CON (p &lt; 0.001). Carbohydrate oxidation was increased by DCA, 0.037 ± 0.017 g min<sup>–1</sup> (p &lt; 0.05) at 3 h with no change observed in CON. UCP3 and PGC1α mRNA expression were induced in CON (as a response to continued fasting) but this was attenuated by DCA. Akt phosphorylation and the expression of other metabolic genes and transcription factors were unchanged throughout the intervention. <i>Conclusion:</i> It is concluded that PDC flux can be increased independently of plasma substrate availability, without causing downstream alterations to metabolic gene expression in the short term.
DOI: 10.6084/m9.figshare.5241262.v1
title: Erratum: Motor Cortex Stimulation for Trigeminal Neuropathic or Deafferentation Pain: An Institutional Case Series Experience

description: <i>Background:</i> Trigeminal neuropathy is a rare, devastating condition that can be intractable and resistant to treatment. When medical treatment fails, invasive options are limited. Motor cortex stimulation (MCS) is a relatively recent technique introduced to treat central neuropathic pain. The use of MCS to treat trigeminal neuropathic or deafferentation pain is not widespread and clinical data in the medical literature that demonstrate efficacy are limited. <i>Method:</i> We retrospectively reviewed patients with trigeminal neuropathic or trigeminal deafferentation pain who were treated at the Oregon Health & Science University between 2001 and 2008 by 1 neurosurgeon using MCS. <i>Results:</i> Eight of 11 patients (3 male, 8 female) underwent successful permanent implantation of an MCS system. All 8 patients reported initial satisfactory pain control. Three failed to experience continued pain control (6 months of follow-up). Five continued to experience long-term pain control (mean follow-up, 33 months). Average programming sessions were 2.2/year (all 8 patients) and 1.55/year (5 patients who sustained long-term pain control). Patients with anesthesia dolorosa or trigeminal deafferentation pain who had previously undergone ablative trigeminal procedures responded poorly to MCS. We encountered no perioperative complications. <i>Conclusion:</i> MCS is a safe and potentially effective therapy in certain patients with trigeminal neuropathy.
DOI: 10.6084/m9.figshare.5241268.v1
title: Erratum: The Relationship between Insight and Uncertainty in Obsessive-Compulsive Disorder

description: <i>Background:</i> The aim of this study was to investigate the relationship between the levels of insight and checking-related uncertainty in patients with obsessive-compulsive disorder (OCD). <i>Sampling and Methods:</i> Twenty OCD patients with checking compulsions and without current comorbidity were recruited. We used an experimental paradigm that gave subjects the opportunity to check during a decision-making task, thereby allowing for the calculation of a response time index (RTI) as the ‘uncertainty cost’ during decision-making. The level of insight was assessed with the Brown Assessment of Beliefs Scale (BABS). <i>Results:</i> Regression analyses indicated a significant positive correlation between RTI and BABS scores (r = 0.49). <i>Conclusions:</i> The level of insight is related to cognitive characteristics underlying OCD symptoms, in particular, checking-related uncertainty in checking OCD patients. <i>Study Limitations:</i> The absence of a comparison group and the low number of included patients are the main limitations of the present study.
DOI: 10.6084/m9.figshare.5241265.v1
title: Erratum: Chromosome Mapping of H1 Histone and 5S rRNA Gene Clusters in Three Species of <i>Astyanax </i>(Teleostei, Characiformes)

description: We report here on the physical mapping of the H1 histone genes (hisDNA) and the 5S ribosomal DNA (rDNA) in 3 Neotropical fish species of the genus <i>Astyanax</i><i>(A. altiparanae</i>,<i> A. bockmanni </i>and<i> A. fasciatus)</i> and the comparative analysis of the chromosomes bearing these genes. Nucleotide analyses by sequencing of both genes were also performed. The distribution of the H1 histone genes was more conserved than that of the rRNA genes, since these were always located in the pericentromeric regions of 2 chromosome pairs. 5S rDNA was found on one of the pairs that presented an H1 histone cluster; this seems to be a conserved chromosomal feature of the genus <i>Astyanax</i>. In addition, individuals of <i>A. bockmanni </i>and<i> A. fasciatus</i> showed clusters of 5S rDNA on 1 pair of acrocentric chromosomes, not found in <i>A. altiparanae</i>. The results obtained by chromosome mapping as well as by sequencing of both genes showed that <i>A.</i><i>bockmanni</i> is more closely related to <i>A. fasciatus</i> than to <i>A. altiparanae</i>. The results allow the characterization of cytogenetic markers for improved elucidation of the processes involved in karyotype differentiation of fish genomes.
DOI: 10.6084/m9.figshare.5241250
title: Collected raw field data

description: Contains:<br><br>- Collected timed bird count estimates (10min ~ 50m radius) in the Taita hills ( Species.xls).<br><br>- Site information including local land use, date, location (lon/lat) all within the SiteData sheet (SiteData.xls). <br><br>Feel free to contact me if in doubt or unsure about any value.<br><br>If you reuse this data for any analysis, please cite the original paper:<br>Jung, M., Hill, S. L. L., Platts, P. J., Marchant, R., Siebert, S., Fournier, A., Munyekenye, F. B., Purvis, A., Burgess, N. D. and Newbold, T. (2016), Local factors mediate the response of biodiversity to land use on two African mountains. Anim Conserv. doi:10.1111/acv.12327
DOI: 10.6084/m9.figshare.5241253
title: Erratum: Lobular Breast Cancer in Men: Case Report and Review of the Literature

description: <i>Background:</i> Lobular breast cancer in men is an extremely infrequent occurrence due to the lack of lobules and acini in the male breast. Such a rare case is described here. <i>Case Report:</i> A 74-year-old patient presented with a sizeable lesion in the right breast, which proved to be a lobular carcinoma. Genetic studies excluded Klinefelter’s syndrome, though revealing an interesting genetic multiformity feature. This case represented a lobular carcinoma in a genotypically male patient under no exogenous or endogenous estrogenic influence. <i>Conclusions:</i> The increasing number of male lobular breast cancer cases should be explored more extensively with particular emphasis placed on causally related genetic and hormonal factors.
DOI: 10.6084/m9.figshare.5241256
title: Erratum: Sentinel Lymph Node Biopsy Status Is a Key Parameter to Stratify the Prognostic Heterogeneity of Malignant Melanoma in High-Risk Tumors &gt;4.0 mm

description: <i>Background:</i> The value of sentinel lymph node biopsy (SLNB) as a useful strategy to assess the risk of future metastasis in high-risk melanomas (&gt;4.0 mm) is controversially discussed. <i>Objectives:</i> In a single-center retrospective study, the prognostic relevance of SLNB and other risk factors in the subgroup of melanomas &gt;4.0 mm was investigated and compared to previously published results. <i>Methods:</i> Using Kaplan-Meier estimates and Cox regressions, we assessed the prognostic relevance of SLNB in our subcohort of 87 patients with thick melanomas &gt;4.0 mm (T4). The mean follow-up for this subgroup was 51 months. We compared SLN value as compared to ulceration. <i>Results:</i> SLN and ulceration, analyzed as separate risk factors as well as their combination, predicted a highly reduced life expectancy in terms of recurrence-free survival (RFS) in our cohort of patients. SLN, but not ulceration, also predicted overall survival (OS). <i>Conclusions:</i> Positive SLNB is an essential predictor of RFS and OS in T4 melanoma patients, whereas ulceration lacked significance with respect to OS in our cohort. Our data thus suggest the routine use of SLNB also for T4 melanoma and may therefore allow to optimize risk-stratified therapeutic regimens.
DOI: 10.6084/m9.figshare.5241247
title: Erratum: β-Globin Gene Cluster Haplotypes in a Cohort of 221 Children with Sickle Cell Anemia or Sβ⁰-Thalassemia and Their Association with Clinical and Hematological Features

description: <i>Background/Aims:</i> β<sup>S</sup>-Haplotype prevalence and its associations with clinical and hematological characteristics were assessed in Brazilian children with sickle cell anemia or Sβ⁰-thalassemia. <i>Methods:</i> A retrospective randomized cohort study was undertaken with 208 SS and 13 Sβ⁰-thalassemia children derived from the Newborn Screening Program of the state of Minas Gerais. β<sup>S</sup>-Haplotypes were determined by PCR-RFLP. <i>Results:</i> Thirty-nine percent of the SS subjects had the CAR/CAR genotype, 33% had CAR/Ben, 24% had Ben/Ben, 1% had CAR/Atp, 1% had Ben/Atp, and 1% had Arab-Indian/Ben; 1% could not be characterized. Of the Sβ⁰-thalassemia children, 5 were CAR/undefined, 2 were Ben/undefined, and 1 was CAM/undefined. There was no significant association between β<sup>S</sup>-haplotypes and the total Hb, Hb F, MCV, MCH, WBC, and reticulocyte count among the SS children. Likewise, no significant association was detected between β<sup>S</sup>-haplotypes and the frequency of acute chest syndrome episodes, blood transfusions, splenic sequestration, or cerebrovascular disease (high-risk/conditional transcranial Doppler ultrasonography or clinical stroke). A limited number of Sβ⁰-thalassemia children precluded valid analyses. <i>Conclusions:</i> The prevalence of β<sup>S</sup>-haplotypes in this study is in agreement with the historical records of African slaves brought to the state of Minas Gerais. Furthermore, β<sup>S</sup>-haplotypes CAR and Ben were not associated with any analyzed feature of children with sickle cell anemia.
DOI: 10.6084/m9.figshare.5241244
title: Erratum: Significant Associations between <i>CCL5 </i>Gene Polymorphisms and Post-Transplantational Diabetes Mellitus in Korean Renal Allograft Recipients

description: <i>Background:</i> Post-transplantational diabetes mellitus (PTDM) is a serious metabolic complication that may follow renal transplantation. The expression of chemokine (C-C motif) ligand 5 (CCL5) is inversely related to pancreatic β-cell function; thus, specific <i>CCL5</i> gene polymorphisms are considered to be risk factors for diabetes. In this study, we investigated the association between <i>CCL5</i> gene polymorphisms and the occurrence of PTDM in Korean patients who had undergone renal transplants. <i>Methods:</i> A total of 311 patients who had received kidney transplants without a prior history of diabetes were included. Three single nucleotide polymorphisms (SNPs) of the <i>CCL5 </i>gene were genotyped from genomic DNA with direct sequencing. <i>Results:</i> PTDM developed in 56 patients (18.0%). The results showed that the allele frequencies of <i>CCL5</i> gene polymorphisms, rs2107538*T, rs2280789*C and rs3817655*A were significantly higher in the patients with PTDM than in those without PTDM. In multiple logistic regression analysis, 3 SNPs (rs2107538, rs2280789 and rs3817655) of the <i>CCL5</i> gene were significantly associated with the development of PTDM in the codominant 2 and recessive models. Among haplotypes of the 3 polymorphisms, the frequency of the TCA haplotype was significantly higher in patients with PTDM than in those without PTDM. <i>Conclusions:</i> Our results indicated that genetic polymorphisms of the <i>CCL5</i> gene were associated with PTDM, suggesting that the <i>CCL5</i> gene might confer susceptibility to PTDM in patients who receive renal transplants.
DOI: 10.6084/m9.figshare.5241253.v1
title: Erratum: Lobular Breast Cancer in Men: Case Report and Review of the Literature

description: <i>Background:</i> Lobular breast cancer in men is an extremely infrequent occurrence due to the lack of lobules and acini in the male breast. Such a rare case is described here. <i>Case Report:</i> A 74-year-old patient presented with a sizeable lesion in the right breast, which proved to be a lobular carcinoma. Genetic studies excluded Klinefelter’s syndrome, though revealing an interesting genetic multiformity feature. This case represented a lobular carcinoma in a genotypically male patient under no exogenous or endogenous estrogenic influence. <i>Conclusions:</i> The increasing number of male lobular breast cancer cases should be explored more extensively with particular emphasis placed on causally related genetic and hormonal factors.
DOI: 10.6084/m9.figshare.5241256.v1
title: Erratum: Sentinel Lymph Node Biopsy Status Is a Key Parameter to Stratify the Prognostic Heterogeneity of Malignant Melanoma in High-Risk Tumors &gt;4.0 mm

description: <i>Background:</i> The value of sentinel lymph node biopsy (SLNB) as a useful strategy to assess the risk of future metastasis in high-risk melanomas (&gt;4.0 mm) is controversially discussed. <i>Objectives:</i> In a single-center retrospective study, the prognostic relevance of SLNB and other risk factors in the subgroup of melanomas &gt;4.0 mm was investigated and compared to previously published results. <i>Methods:</i> Using Kaplan-Meier estimates and Cox regressions, we assessed the prognostic relevance of SLNB in our subcohort of 87 patients with thick melanomas &gt;4.0 mm (T4). The mean follow-up for this subgroup was 51 months. We compared SLN value as compared to ulceration. <i>Results:</i> SLN and ulceration, analyzed as separate risk factors as well as their combination, predicted a highly reduced life expectancy in terms of recurrence-free survival (RFS) in our cohort of patients. SLN, but not ulceration, also predicted overall survival (OS). <i>Conclusions:</i> Positive SLNB is an essential predictor of RFS and OS in T4 melanoma patients, whereas ulceration lacked significance with respect to OS in our cohort. Our data thus suggest the routine use of SLNB also for T4 melanoma and may therefore allow to optimize risk-stratified therapeutic regimens.