DOI: 10.7267/8623j475z
title: Dataset: Genetic diversity, reproductive capacity and alkaloids content in three endemic Alkanna species

description: This data includes studies on three endemic species of Alkanet, Alkanna (Alkanna primuliflora, A. stribrnyi and A. graeca) from the family Boraginacea, distributed in Bulgaria. We have presented raw data (photographs, chromatograms and graphs) from embryological, genetic and phytochemical studies. The three Alkanna species are Balkan endemics and have a limited distribution on the Balkan Peninsula. The limited distribution of the species is often associated with problems in the reproductive capacity of the species, which is why the embryological examination was performed. The morphological features that distinguish the three species vary, they are unclear, and sometimes transitional forms are observed between the three species, which is why DNA extraction and Inter-simple sequence repeat (ISSR) amplification product analysis were used. The data included GC of the crude mixtures of alkaloids of Alkanna primuliflora, A. graeca and A. stribrnyi and MS spectra of the identified pyrrolizidine alkaloids (PAs). 1) For the embryological examination (male and female generative sphere, pollen viability and seed germ) a classical paraffin methodology was applied. For the embryological study, we collected flowers and buds at different stages of development from the three species from different populations. The material was processed according to classical methods and documented with photographic material. The main embryological structures and processes in the male and female generative sphere were established after observations using LM “Olympus” CX2. The microphotographs were made using an “Infinity lite” digital camera 1.4 Mpx. The presented photos are unprocessed and are used to describe the male and female and male generative spheres of the three species. The boards in the article are based on these photos. A description is provided to the attached photos in this document. 2) DNA extraction and Inter-simple sequences repeat (ISSR) amplification product analysis of the collected leaves of the three species and applied ISSR patterns of 15 Alkanna individuals belonging to Alkanna primuliflora (1-5), Alkanna graeca (6-10) and Alkanna stribrnyi 11-15) generated by primers (AC) 8YT (left) and (AG) 10C (right), respectively. The DNA marker is GeneRuler 100 bp plus. Based on statistical data processing (Principal Coordinate Analysis (PCoA) and Hierarchical Analysis of Molecular Variance (AMOVA) in GenALEx software) were conducted to show the genetic relationships among the Alkanna populations on the basis of Nei’s genetic distances. The DNA fragments were visualized under UV light and further analyzed with a video image analyzer (BioImaging Systems, Cambridge, UK). 3) Extraction of plant material and qualitative analysis of alkaloids. From GC-MS Analysis of alkaloids identified from Alkanna primuliflora, A. graeca and A. stribrnyi, chromatograms were used, which were not used in the article. They represent a crude alkaloid mixture of the three species. Based on mass spectral fragmentation with standard reference spectra from the database, alkaloids were identified in all three species.