cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7941662
title: Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing

description: Transforming growth factor beta 1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractured tibias were measured by immunohistochemistry and quantitative reverse-transcription polymerase chain reaction, weekly for the first 5 weeks post-fracture. Mechanical parameters (bending rigidity, torsional rigidity, destruction torque) of the healing bones were also assessed at 3, 4, and 5 weeks post-fracture, after the rats were sacrificed. The bending rigidity, torsional rigidity and destruction torque of the two groups increased continuously during the healing process. The diabetes group had lower mean values for bending rigidity, torsional rigidity and destruction torque compared with the control group (P&lt;0.05). TGF-β1 and BMP-2 expression were significantly lower (P&lt;0.05) in the control group than in the diabetes group at postoperative weeks 1, 2, and 3. Peak levels of TGF-β1 and BMP-2 expression were delayed by 1 week in the diabetes group compared with the control group. Our results demonstrate that there was a delayed recovery in the biomechanical function of the fractured bones in diabetic rats. This delay may be associated with a delayed expression of the growth factors TGF-β1 and BMP-2.
DOI: 10.6084/m9.figshare.7941668.v1
title: Biomedical knowledge in Mexico during the Cold War and its impact in pictorial representations of Homo sapiens and racial hierarchies

description: Abstract This paper provides an overview of the state of Mexican genetics and biomedical knowledge during the second half of the twentieth century, as well as its impact on the visual representation of human groups and racial hierarchies, based on social studies of scientific imaging and visualization (SIV) and theoretical concepts and methods. It also addresses the genealogy and shifts of the concept of race and racialization of Mexican bodies, concluding with the novel visual culture that resulted from genetic knowledge merged with the racist phenomenon in the second half of the twentieth century in Mexico.
DOI: 10.6084/m9.figshare.7941674.v1
title: Nurse researchers and nursing assistants: construction and projection of polymorphous identities

description: ABSTRACT Objective: To understand the meanings that nurse researchers and nursing assistants attribute to one another, and to the development of research produced by Nursing. Method: Qualitative research. The Grounded Theory and the Complexity Theory were used as theoretical and methodological frameworks, respectively. Ten nursing assistants and six research nurses participated in the research. The semi-structured interview was used as a data collection technique. The results were categorized by comparative analysis and validated by ten judges. Results: The valuation of nursing care research as well as interactions between research development and nursing care may be related to the way nursing assistants and nurse researchers perceive themselves. These perceptions are multifaceted. Conclusion: The meanings nurse researchers and nursing assistants attribute to each other reveal mechanisms of closeness and distance between these actors, as well as between research and nursing care.
DOI: 10.6084/m9.figshare.7941656
title: Negative emotions affect postoperative scores for evaluating functional knee recovery and quality of life after total knee replacement

description: This study aimed to determine whether psychological factors affect health-related quality of life (HRQL) and recovery of knee function in total knee replacement (TKR) patients. A total of 119 TKR patients (male: 38; female: 81) completed the Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), State Trait Anxiety Inventory (STAI), Eysenck Personality Questionnaire-revised (EPQR-S), Knee Society Score (KSS), and HRQL (SF-36). At 1 and 6 months after surgery, anxiety, depression, and KSS scores in TKR patients were significantly better compared with those preoperatively (P&lt;0.05). SF-36 scores at the sixth month after surgery were significantly improved compared with preoperative scores (P&lt;0.001). Preoperative Physical Component Summary Scale (PCS) and Mental Component Summary Scale (MCS) scores were negatively associated with extraversion (E score) (B=-0.986 and -0.967, respectively, both P&lt;0.05). Postoperative PCS and State Anxiety Inventory (SAI) scores were negatively associated with neuroticism (N score; B=-0.137 and -0.991, respectively, both P&lt;0.05). Postoperative MCS, SAI, Trait Anxiety Inventory (TAI), and BAI scores were also negatively associated with the N score (B=-0.367, -0.107, -0.281, and -0.851, respectively, all P&lt;0.05). The KSS function score at the sixth month after surgery was negatively associated with TAI and N scores (B=-0.315 and -0.532, respectively, both P&lt;0.05), but positively associated with the E score (B=0.215, P&lt;0.05). The postoperative KSS joint score was positively associated with postoperative PCS (B=0.356, P&lt;0.05). In conclusion, for TKR patients, the scores used for evaluating recovery of knee function and HRQL after 6 months are inversely associated with the presence of negative emotions.
DOI: 10.6084/m9.figshare.7941662.v1
title: Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing

description: Transforming growth factor beta 1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractured tibias were measured by immunohistochemistry and quantitative reverse-transcription polymerase chain reaction, weekly for the first 5 weeks post-fracture. Mechanical parameters (bending rigidity, torsional rigidity, destruction torque) of the healing bones were also assessed at 3, 4, and 5 weeks post-fracture, after the rats were sacrificed. The bending rigidity, torsional rigidity and destruction torque of the two groups increased continuously during the healing process. The diabetes group had lower mean values for bending rigidity, torsional rigidity and destruction torque compared with the control group (P&lt;0.05). TGF-β1 and BMP-2 expression were significantly lower (P&lt;0.05) in the control group than in the diabetes group at postoperative weeks 1, 2, and 3. Peak levels of TGF-β1 and BMP-2 expression were delayed by 1 week in the diabetes group compared with the control group. Our results demonstrate that there was a delayed recovery in the biomechanical function of the fractured bones in diabetic rats. This delay may be associated with a delayed expression of the growth factors TGF-β1 and BMP-2.
DOI: 10.6084/m9.figshare.7941665.v1
title: Microglia and neurons in the hippocampus of migratory sandpipers

description: The semipalmated sandpiper Calidris pusilla and the spotted sandpiper Actitis macularia are long- and short-distance migrants, respectively. C. pusilla breeds in the sub-arctic and mid-arctic tundra of Canada and Alaska and winters on the north and east coasts of South America. A. macularia breeds in a broad distribution across most of North America from the treeline to the southern United States. It winters in the southern United States, and Central and South America. The autumn migration route of C. pusilla includes a non-stop flight over the Atlantic Ocean, whereas autumn route of A. macularia is largely over land. Because of this difference in their migratory paths and the visuo-spatial recognition tasks involved, we hypothesized that hippocampal volume and neuronal and glial numbers would differ between these two species. A. macularia did not differ from C. pusilla in the total number of hippocampal neurons, but the species had a larger hippocampal formation and more hippocampal microglia. It remains to be investigated whether these differences indicate interspecies differences or neural specializations associated with different strategies of orientation and navigation.
DOI: 10.6084/m9.figshare.7941653
title: Effect of (+)-dehydrofukinone on GABAA receptors and stress response in fish model

description: (+)-Dehydrofukinone (DHF) is a major component of the essential oil of Nectandra grandiflora (Lauraceae), and exerts a depressant effect on the central nervous system of fish. However, the neuronal mechanism underlying DHF action remains unknown. This study aimed to investigate the action of DHF on GABAA receptors using a silver catfish (Rhamdia quelen) model. Additionally, we investigated the effect of DHF exposure on stress-induced cortisol modulation. Chemical identification was performed using gas chromatography-mass spectrometry and purity was evaluated using gas chromatography with a flame ionization detector. To an aquarium, we applied between 2.5 and 50 mg/L DHF diluted in ethanol, in combination with 42.7 mg/L diazepam. DHF within the range of 10-20 mg/L acted collaboratively in combination with diazepam, but the sedative action of DHF was reversed by 3 mg/L flumazenil. Additionally, fish exposed for 24 h to 2.5-20 mg/L DHF showed no side effects and there was sustained sedation during the first 12 h of drug exposure with 10-20 mg/L DHF. DHF pretreatment did not increase plasma cortisol levels in fish subjected to a stress protocol. Moreover, the stress-induced cortisol peak was absent following pretreatment with 20 mg/L DHF. DHF proved to be a relatively safe sedative or anesthetic, which interacts with GABAergic and cortisol pathways in fish.
DOI: 10.6084/m9.figshare.7941656.v1
title: Negative emotions affect postoperative scores for evaluating functional knee recovery and quality of life after total knee replacement

description: This study aimed to determine whether psychological factors affect health-related quality of life (HRQL) and recovery of knee function in total knee replacement (TKR) patients. A total of 119 TKR patients (male: 38; female: 81) completed the Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), State Trait Anxiety Inventory (STAI), Eysenck Personality Questionnaire-revised (EPQR-S), Knee Society Score (KSS), and HRQL (SF-36). At 1 and 6 months after surgery, anxiety, depression, and KSS scores in TKR patients were significantly better compared with those preoperatively (P&lt;0.05). SF-36 scores at the sixth month after surgery were significantly improved compared with preoperative scores (P&lt;0.001). Preoperative Physical Component Summary Scale (PCS) and Mental Component Summary Scale (MCS) scores were negatively associated with extraversion (E score) (B=-0.986 and -0.967, respectively, both P&lt;0.05). Postoperative PCS and State Anxiety Inventory (SAI) scores were negatively associated with neuroticism (N score; B=-0.137 and -0.991, respectively, both P&lt;0.05). Postoperative MCS, SAI, Trait Anxiety Inventory (TAI), and BAI scores were also negatively associated with the N score (B=-0.367, -0.107, -0.281, and -0.851, respectively, all P&lt;0.05). The KSS function score at the sixth month after surgery was negatively associated with TAI and N scores (B=-0.315 and -0.532, respectively, both P&lt;0.05), but positively associated with the E score (B=0.215, P&lt;0.05). The postoperative KSS joint score was positively associated with postoperative PCS (B=0.356, P&lt;0.05). In conclusion, for TKR patients, the scores used for evaluating recovery of knee function and HRQL after 6 months are inversely associated with the presence of negative emotions.
DOI: 10.6084/m9.figshare.7941659.v1
title: Overexpression of myeloid differentiation protein 88 in mice induces mild cardiac dysfunction, but no deficit in heart morphology

description: Cardiac remodeling involves changes in heart shape, size, structure, and function after injury to the myocardium. The proinflammatory adaptor protein myeloid differentiation protein 88 (MyD88) contributes to cardiac remodeling. To investigate whether excessive MyD88 levels initiate spontaneous cardiac remodeling at the whole-organism level, we generated a transgenic MyD88 mouse model with a cardiac-specific promoter. MyD88 mice (male, 20-30 g, n=∼80) were born at the expected Mendelian ratio and demonstrated similar morphology of the heart and cardiomyocytes with that of wild-type controls. Although heart weight was unaffected, cardiac contractility of MyD88 hearts was mildly reduced, as shown by echocardiographic examination, compared with wild-type controls. Moreover, the cardiac dysfunction phenotype was associated with elevation of ANF and BNP expression. Collectively, our data provide novel evidence of the critical role of balanced MyD88 signaling in maintaining physiological function in the adult heart.
DOI: 10.6084/m9.figshare.7941644
title: COMBUSTION SIMULATION OF HYDROXYL-TERMINATED LIQUID POLYBUTADIENE, AMMONIUM PERCHLORATE AND ALUMINUM IN COMPOSITE SOLID PROPELLANT FORMULATIONS

description: The present work presents combustion simulations of hydroxyl-terminated liquid polybutadiene loaded with ammonium perchlorate and aluminum, using Chemkin software and the “Plug Flow” type reactor. These materials are widely used in solid composite propellant formulations. Through this study, the effect of parameters of axial velocity, equivalence ratio, pressure and temperature during the firing of the propellant were discussed. The objective of this work was to discuss how to define the best parameter value based on the obtained results, and thus generate optimized formulations.
DOI: 10.6084/m9.figshare.7941647
title: A Review of Using Conductive Composite Materials in Solving Lightening Strike and Ice Accumulation Problems in Aviation

description: ABSTRACT: There are many problems facing aircraft in the air during flight, such as lightning strikes and ice accumulation on aircraft surfaces. These problems usually reduce aircraft efficiency and lead to serious accidents and fatalities. However, the current protection systems used to solve these problems of aircraft represent excessive energy usage, a hazard to the environment, and they are generally bulky, heavy and costly. Therefore, there are new conductive composites containing an embedded layer of conductive fibers such as graphene and carbon nanotube designed to carry lightning currents, in addition to that, there is a new deicing heater element made of graphene nanoribbons films to be used in ice protection systems. This paper presents a review of some problems facing aircraft in the air, such as lightning and ice accumulation on the surfaces of the aircraft and the significant efforts that have been exerted to address and solve these issues. Also, this paper reviews the contribution of composite materials in reducing the weight of the aircraft and fuel consumption as well as increasing the efficiency of aircraft. This paper also will review the conductive composite materials and its application for aviation, in addition to their contribution to solving the most important problems in aviation.
DOI: 10.6084/m9.figshare.7941650
title: MONITORING OF BIOSURFACTANT PRODUCTION BY Bacillus subtilis USING BEET PEEL AS CULTURE MEDIUM VIA THE DEVELOPMENT OF A NEURAL SOFT-SENSOR IN AN ELECTRONIC SPREADSHEET

description: ABSTRACT This work investigated the combination of agitation and aeration conditions in a bench-bioreactor to identify the optimal biosurfactant production from substrate based on beet peel and glycerol from a biodiesel process. Thus, a central composite rotatable design (CCRD) and responses were evaluated by response surface methodology (RSM) modeling. The optimal operation values determined were 200 rpm (agitation) and 0.5 vvm (aeration), reaching values of 1931.2 mg/L of crude biosurfactant concentration and 28.37 mN/m of surface tension. For the development of a mathematical model based on an artificial neural network (ANN), the experimental data from each run (CCRD) of the bioreactor were used. The results indicated a topology of 6-6-1 neurons with an excellent predictive capacity of biosurfactant concentration: dispersion plot with R2 of 0.995, and error criteria SSE of 0.31, MSE of 7.29×10-4 and RSME of 2.7×10-2. A soft sensor was then designed in an electronic spreadsheet, computing the biosurfactant production from secondary measurements. Furthermore, the produced biosurfactant showed the ability to remediate oil spreading, evaluated through the appearance of clear zones on the surface of water covered with oil, and also from high emulsification indexes obtained on most of the solvents tested, such as toluene (~65%).
DOI: 10.6084/m9.figshare.7941647.v1
title: A Review of Using Conductive Composite Materials in Solving Lightening Strike and Ice Accumulation Problems in Aviation

description: ABSTRACT: There are many problems facing aircraft in the air during flight, such as lightning strikes and ice accumulation on aircraft surfaces. These problems usually reduce aircraft efficiency and lead to serious accidents and fatalities. However, the current protection systems used to solve these problems of aircraft represent excessive energy usage, a hazard to the environment, and they are generally bulky, heavy and costly. Therefore, there are new conductive composites containing an embedded layer of conductive fibers such as graphene and carbon nanotube designed to carry lightning currents, in addition to that, there is a new deicing heater element made of graphene nanoribbons films to be used in ice protection systems. This paper presents a review of some problems facing aircraft in the air, such as lightning and ice accumulation on the surfaces of the aircraft and the significant efforts that have been exerted to address and solve these issues. Also, this paper reviews the contribution of composite materials in reducing the weight of the aircraft and fuel consumption as well as increasing the efficiency of aircraft. This paper also will review the conductive composite materials and its application for aviation, in addition to their contribution to solving the most important problems in aviation.
DOI: 10.6084/m9.figshare.7941653.v1
title: Effect of (+)-dehydrofukinone on GABAA receptors and stress response in fish model

description: (+)-Dehydrofukinone (DHF) is a major component of the essential oil of Nectandra grandiflora (Lauraceae), and exerts a depressant effect on the central nervous system of fish. However, the neuronal mechanism underlying DHF action remains unknown. This study aimed to investigate the action of DHF on GABAA receptors using a silver catfish (Rhamdia quelen) model. Additionally, we investigated the effect of DHF exposure on stress-induced cortisol modulation. Chemical identification was performed using gas chromatography-mass spectrometry and purity was evaluated using gas chromatography with a flame ionization detector. To an aquarium, we applied between 2.5 and 50 mg/L DHF diluted in ethanol, in combination with 42.7 mg/L diazepam. DHF within the range of 10-20 mg/L acted collaboratively in combination with diazepam, but the sedative action of DHF was reversed by 3 mg/L flumazenil. Additionally, fish exposed for 24 h to 2.5-20 mg/L DHF showed no side effects and there was sustained sedation during the first 12 h of drug exposure with 10-20 mg/L DHF. DHF pretreatment did not increase plasma cortisol levels in fish subjected to a stress protocol. Moreover, the stress-induced cortisol peak was absent following pretreatment with 20 mg/L DHF. DHF proved to be a relatively safe sedative or anesthetic, which interacts with GABAergic and cortisol pathways in fish.
DOI: 10.6084/m9.figshare.7941644.v1
title: COMBUSTION SIMULATION OF HYDROXYL-TERMINATED LIQUID POLYBUTADIENE, AMMONIUM PERCHLORATE AND ALUMINUM IN COMPOSITE SOLID PROPELLANT FORMULATIONS

description: The present work presents combustion simulations of hydroxyl-terminated liquid polybutadiene loaded with ammonium perchlorate and aluminum, using Chemkin software and the “Plug Flow” type reactor. These materials are widely used in solid composite propellant formulations. Through this study, the effect of parameters of axial velocity, equivalence ratio, pressure and temperature during the firing of the propellant were discussed. The objective of this work was to discuss how to define the best parameter value based on the obtained results, and thus generate optimized formulations.
DOI: 10.6084/m9.figshare.7941650.v1
title: MONITORING OF BIOSURFACTANT PRODUCTION BY Bacillus subtilis USING BEET PEEL AS CULTURE MEDIUM VIA THE DEVELOPMENT OF A NEURAL SOFT-SENSOR IN AN ELECTRONIC SPREADSHEET

description: ABSTRACT This work investigated the combination of agitation and aeration conditions in a bench-bioreactor to identify the optimal biosurfactant production from substrate based on beet peel and glycerol from a biodiesel process. Thus, a central composite rotatable design (CCRD) and responses were evaluated by response surface methodology (RSM) modeling. The optimal operation values determined were 200 rpm (agitation) and 0.5 vvm (aeration), reaching values of 1931.2 mg/L of crude biosurfactant concentration and 28.37 mN/m of surface tension. For the development of a mathematical model based on an artificial neural network (ANN), the experimental data from each run (CCRD) of the bioreactor were used. The results indicated a topology of 6-6-1 neurons with an excellent predictive capacity of biosurfactant concentration: dispersion plot with R2 of 0.995, and error criteria SSE of 0.31, MSE of 7.29×10-4 and RSME of 2.7×10-2. A soft sensor was then designed in an electronic spreadsheet, computing the biosurfactant production from secondary measurements. Furthermore, the produced biosurfactant showed the ability to remediate oil spreading, evaluated through the appearance of clear zones on the surface of water covered with oil, and also from high emulsification indexes obtained on most of the solvents tested, such as toluene (~65%).
DOI: 10.6084/m9.figshare.7941632
title: gVCF_NA12827

description: 1000 Genomes gVCF mapped to hs37d5 for NA12827. Complete collection: https://doi.org/10.6084/m9.figshare.c.4414307
DOI: 10.6084/m9.figshare.7941638
title: Overview of Zika virus (ZIKV) infection in regards to the Brazilian epidemic

description: Zika virus (ZIKV), a mosquito-borne flavivirus, belongs to the Flaviviridae family, genus Flavivirus. ZIKV was initially isolated in 1947 from a sentinel monkey in the Zika forest, Uganda. Little clinical importance was attributed to ZIKV, once only few symptomatic cases were reported in some African and Southeast Asiatic countries. This situation changed in 2007, when a large outbreak was registered on the Yap Island, Micronesia, caused by the Asian ZIKV lineage. Between 2013 and 2014, ZIKV spread explosively and caused many outbreaks in different islands of the Southern Pacific Ocean and in 2015 autochthonous transmission was reported in Brazil. Currently, Brazil is the country with the highest number of ZIKV-positive cases in Latin America. Moreover, for the first time after the discovery of ZIKV, the Brazilian scientists are studying the possibility for the virus to cause severe congenital infection related to microcephaly and serious birth defects due to the time-spatial coincidence of the alarming increase of newborns with microcephaly and the Brazilian ZIKV epidemic. The present review summarizes recent information for ZIKV epidemiology, clinical picture, transmission, diagnosis and the consequences of this emerging virus in Brazil.