cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/hsecz-t1z78
title: Shewanella oneidensis dFlgH

description: Raw data files of Shewanella oneidensis dFlgH
description: <strong>Tilt Series Date:</strong> 2018-05-27</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Jeff Granlick (Uni. of Minnesota) supplied the mutant cells.</p>
<strong>Sample Preparation:</strong> Samples were prepared from overnight LB culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-35/rawdata/</p>tilt41.mrc,  Tilt Series (Pixel Size 0.57 nm), 1.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-35/rawdata/tilt41.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt41_full.rec,  Reconstruction (Pixel Size 2.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-35/3dimage_93357/tilt41_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8gdgf-qmc98
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-17</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-91/rawdata/</p>targ89.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-91/rawdata/targ89.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ89_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-91/3dimage_99666/targ89_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cxm49-qfy79
title: Legionella delta DotOB

description: DotF-GFP background
description: <strong>Tilt Series Date:</strong> 2019-02-13</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph Vogel</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase</p>
<strong>Sample Preparation:</strong> 10 nm gold beads, broad vitrobot, R2/2 200 mesh copper quantifoil grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-62/rawdata/</p>20190213_deltaOB_97.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-62/rawdata/20190213_deltaOB_97.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190213_deltaOB_97_part61_8.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-62/3dimage_101512/20190213_deltaOB_97_part61_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/95hej-t6x18
title: cem1-1_dummy_3

description: CEM1-1 - Arabidopsis cultured cells cell wall Titan w/ phase plate
description: <strong>Tilt Series Date:</strong> 2018-04-04</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Arabidopsis thaliana</p>
<strong>Strain:</strong> Col-0</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 0.0 eV/Å²,  defocus: -3.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> FEI Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mark Ladinsky: cryosectionning Songye: data acquisition</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cryosectioning done by Mark Ladinsky</p>
<strong>Sample Preparation:</strong> Feb21st HPF - Cultured PSD cells - 40% dextran</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-04-4/rawdata/</p>Dummy_3_orig.st,  Tilt Series (Pixel Size 0.35 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-04-4/rawdata/Dummy_3_orig.st"
        > <i class="download icon"></i> Download </a></p>

        Dummy_3.rec,  Reconstruction (Pixel Size 1.46 nm), 216.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-04-4/3dimage_101694/Dummy_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8tbss-gkq89
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-20</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, WT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-31/rawdata/</p>targ67.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-31/rawdata/targ67.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ67_part123_3.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-31/3dimage_101220/targ67_part123_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6q5dz-mzs85
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-4/rawdata/</p>caulobacter_na1000_wt_004_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-4/rawdata/caulobacter_na1000_wt_004_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_004_af.mrc.mdoc,  814.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-4/rawdata/caulobacter_na1000_wt_004_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zg2hr-x3p46
title: ec1-1_sq1_cell1

description: IT 1.1s
description: <strong>Tilt Series Date:</strong> 2018-06-28</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 1094</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 165.0 eV/Å²,  defocus: -4.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 3ml culture 37deg, 2hours, OD 1</p>
<strong>Sample Preparation:</strong> On Yiwei's advice: 4uL drop, BF -6, BT 4s, both sides, 100% hum. A lot of the bacterias were blotted away</p>
  wnj2018-06-28-15_slicer19713.jpg: Periplasmic density
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-06-28-15/rawdata/</p>sq1_cell1.mrc,  Tilt Series (Pixel Size 0.54 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-06-28-15/rawdata/sq1_cell1.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq1_cell1_full.rec,  Reconstruction (Pixel Size 2.16 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-06-28-15/3dimage_93075/sq1_cell1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bfsag-2na59
title: P. gingivalis wild type

description: Raw data files of P. gingivalis wild type
description: <strong>Tilt Series Date:</strong> 2020-11-06</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown and harvested from plates</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-216/rawdata/</p>PginWT9_215.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-216/rawdata/PginWT9_215.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT9_215_part41_16.rec,  Reconstruction (Pixel Size 1.332 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-11-06-216/3dimage_115402/PginWT9_215_part41_16.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hm0az-qx744
title: cryo-CLEM TDP-43

description: Osmotic shock (ask Qing Yao)Over expression of TDP-43-GFP
description: <strong>Tilt Series Date:</strong> 2019-06-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> U2OS cells</p>
<strong>Strain:</strong> TDP-43 expression</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Qing Yao</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on gold funder EM grids</p>
<strong>Sample Preparation:</strong> Manual Botting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-7/rawdata/</p>tomography16.mrc,  Tilt Series (Pixel Size 2.678 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-7/rawdata/tomography16.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography16_full.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-7/3dimage_118731/tomography16_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.zxqhdn
title: Occurrence Download

description: A dataset containing 3349 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Fumaria officinalis",
 "Country is Russian Federation",
 "HasCoordinate is true"
 ]
}

 The dataset includes 3349 records from 34 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045294-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/5yxpz-08r39
title: U937 JV9082

description: Legionella pneumophila JV9298 DotF-GFP
description: <strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-3/3dimage_123615/</p>U937_9082_tomo_3_SIRT_1k.rec,  Reconstruction (Pixel Size 1.06 nm), 4.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-3/3dimage_123615/U937_9082_tomo_3_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        U937_9082_tomo_3_bin2.st,  Tilt Series (Pixel Size 0.53 nm), 624.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-3/rawdata/U937_9082_tomo_3_bin2.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2twv7n
title: Occurrence Download

description: A dataset containing 85 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Fumaria officinalis",
 "Country is Slovakia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 85 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045295-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/d0n3t-n2w71
title: U937 JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-12/3dimage_123632/</p>U937_9298_tomo_6_bin2_SIRT_1k.rec,  Reconstruction (Pixel Size 1.06 nm), 4.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-12/3dimage_123632/U937_9298_tomo_6_bin2_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        U937_9298_tomo_6_bin2.st,  Tilt Series (Pixel Size 0.53 nm), 436.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-12/rawdata/U937_9298_tomo_6_bin2.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xjk89-tkf50
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-153/rawdata/</p>caulobacter_na1000_wt_154_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-153/rawdata/caulobacter_na1000_wt_154_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_154_af.mrc.mdoc,  814.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-153/rawdata/caulobacter_na1000_wt_154_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t7y9f-24c56
title: Legionella delta DotO

description: Strain from Yale
description: <strong>Tilt Series Date:</strong> 2019-01-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jun Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase, broad vitrobot, R2/2 quantifoil 200 mesh copper grid</p>
<strong>Sample Preparation:</strong> 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-10/rawdata/</p>tilt_47.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
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        tilt_47_full.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
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