DOI: 10.5061/dryad.mq2p20k
title: Data from: ANDe™ : a fully integrated environmental DNA sampling system

description: 1. Species monitoring from environmental DNA (eDNA) is a powerful new
 technique for natural resource scientists and the number of research
 groups employing eDNA detection is growing rapidly. However, current eDNA
 sampling technologies consist mainly of do-it-yourself solutions, and the
 lack of purpose-built sampling equipment is limiting the efficiency and
 standardization of eDNA studies. 2. Here, we describe the first
 fully-integrated sampling system (ANDeTM) designed by a team of molecular
 ecologists and engineers for high-throughput eDNA sample collection. It
 consists of a backpack portable pump that integrates sensor feedback, a
 pole extension with remote pump controller, custom-made filter housings in
 single-use packets for each sampling site, and onboard sample storage. The
 system is optimized for sampling speed and replicability, while minimizing
 risk of contamination. 3. We present an example pilot study designed to
 identify optimal ANDeTM system parameter values (i.e., pump pressure, flow
 rate, filter pore size, sample volume) in a new sampling environment. We
 identified a peak in filtration efficiency at a flow rate threshold of 1.0
 L/m, and found that 5 µm filters captured significantly more target eDNA
 than 1 µm filters. Results also suggest that high filtration pressures may
 reduce eDNA retention, which implies that pressure should be standardized
 to avoid biasing detection data. 4. Similar to the technological evolution
 of backpack electrofishers, eDNA sampling technology is in the process of
 transitioning from a nascent phase to professionally engineered research
 tools. Such innovations will be essential as eDNA monitoring becomes one
 of the industry standard methods used for species detection and
 management.
description: Flow_DataFlow_Data.csv – Data produced from the Flow Experiment. Fields: pore_size – the filter pore size in µm. particle – the level of suspended particulate matter on the sampling day (high vs low). flow_rate – the user-input flow rate threshold value in L/min replicate – the filter replicate number for each combination of flow_rate, particle, and pore size. vol_filtered – the volume of water filtered in Liters prior to the low-flow alarm. minutes – the elapsed time between filtration initiation and the low-flow alarm (in minutes).Pressure_DataPressure_Data.csv – Data produced from the Pressure Experiment. Fields: pore_size – the filter pore size in µm. pressure – the user-input pressure threshold value in -psi. filter_replicate – the filter replicate number for each combination of pressure and pore size. SQ – The average calculated starting quantity (DNA copies/reaction) from 3 replicate qPCR reactions per sample. total_eDNA – The calculated total mudsnail eDNA per filter sample (DNA copies per filter). vol_filtered – The volume of water filtered in Liters prior to system registering a low-flow alarm. total_eDNA/L – Total eDNA captured per Liter of water filtered (DNA copies per Liter).qPCR_DataqPCR_Data.csv – qPCR results from the Pressure Experiment for each individual qPCR replicate. Fields: pore_size – the filter pore size in µm. pressure – the user-input pressure threshold value in -psi. filter_replicate – the filter replicate number for each combination of pressure and pore size. qPCR_replicate - the qPCR replicate number for each filter sample. SQ – The calculated starting quantity (DNA copies/reaction) from each qPCR replicate. Cq – The Cq value resulting from each qPCR replicate.