DOI: 10.5061/dryad.mcvdnck83
title: Data from: Timing-dependent effects of elevated temperature on reproductive traits in the European corn borer moth

description: Elevated temperature often has life stage-specific effects on ectotherms
 because thermal tolerance varies throughout ontogeny. Impacts of elevated
 temperature may extend beyond the exposed life stage, if developmental
 plasticity causes early exposure to carry over, or if exposure at multiple
 life stages cumulatively produces effects. Reproductive traits may be
 sensitive to different thermal environments experienced during
 development, but such effects have not been comprehensively measured in
 Lepidoptera. In this study, we investigate how elevated temperature at
 different life stages alters reproduction in the European corn borer moth,
 Ostrinia nubilalis. We tested effects of exposure to elevated temperature
 (28°C) separately or additively during larval, pupal, and adult life
 stages compared to control temperatures (23°C). We found that exposure to
 elevated pupal and adult temperature decreased the number of egg clusters
 produced, but exposure limited to a single stage did not significantly
 impact reproductive output. Furthermore, elevated temperature during the
 pupal stage led to a faster transition to the adult stage and elevated
 larval temperature altered synchrony of adult eclosion, either by itself
 or combined with pupal temperature exposure. These results suggest that
 exposure to elevated temperature during development alters reproduction in
 corn borers in multiple ways, including through carry-over and additive
 effects. Additive effects of temperature across life stages are thought to
 be less common than stage-specific or carry-over effects, but our results
 suggest thermal environments experienced at all life stages need to be
 considered when predicting reproductive responses of insects to heatwaves.
description: ## Different.Life.Stages.Final2024.csv contains mating pair data and
 column identifiers are as follows: * MonthYear = Categorical variable of
 year and month pair data collected (included as a random effect in the
 mixed model) * Month = Month pair data collected * Year = Year pair data
 collected * CrossID = ID Number (unique for each pair) * CrossType =
 Within Line or Between Line pairing * Female = Female Line (BE = E line,
 UZ = Z line) * Male = Male Line (BE = E line, UZ = Z line) * EarlyLarval =
 Temperature pair experienced as early larvae (23 degrees, 26 degrees, or
 28 degrees C) * Larval = Temperature pair experienced as larvae: 23
 degrees (control) or 28 degrees(elevated) * PupalTemp = Temperature pair
 experienced as pupae: 23 degrees (control) or 28 degrees (elevated) *
 AdultTemp = Temperature pair experienced as adults: 23 degrees (control)
 or 28 degrees (elevated) * TempID = 3 letter combination indicating
 temperature exposure of pair, using L for 23 degrees and H for 28 degrees
 at each life stage (LLL = Control, HLL = Larval, LHL = Pupal, HLL = Adult,
 LHH = Pupal/Adult, HHH = All) * Day1 = Number of egg clusters 1 day after
 pairing * Day2 = Number of egg clusters 2 days after pairing * Day3 =
 Number of egg clusters 3 days after pairing * Day4 = Number of egg
 clusters 4 days after pairing * Latency5 = Number of days before egg
 clusters were produced with those that never produced eggs being assigned
 day 5 * Day2YN = Did the pair produce eggs by day 2 (0 = No, 1 = Yes) *
 Day3YN = Did the pair produce eggs by day 3 (0 = No, 1 = Yes) * AgeF = Age
 of female at pairing in days * AgeFC = Categorical age of female at
 pairing counted based on day of eclosion as day 0 ("Day1" &lt;=
 1 day, "Day 2"= 2 days, "Plus" &gt;= 3 days) * AgeM =
 Age of male at pairing in days * AgeMC = Categorical age of male at
 pairing counted based on day of eclosion as day 0 ("Day1" &lt;=
 1 day, "Day 2"= 2 days, "Plus" &gt;= 3 days) ##
 BE.Pup.Eclose.Full.Data.txt and UZ.Pup.Eclose.Full.Data.txt contain
 reproductive maturation/eclosion data and column identifiers are as
 follows: * Pupated = Date individual pupated * Eclosed = Date individual
 eclosed * TimePE = Duration of pupal development in days * Line = Line of
 individual (E or Z line) * Sex = Sex of individual (F = female, M = Male)
 * LarvalTemp = Temperature individual experienced as larva: 23 degrees or
 28 degrees (elevated) * PupalTemp = Temperature individual experienced as
 pupa: 23 degrees or 28 degrees (elevated) * TempID = 2 letter combination
 indicating temperature exposure of individual, using L for 23 degrees and
 H for 28 degrees at each life stage (LL = Control, HL = Larval, LH =
 Pupal, HH = All) * FirstDayE = Date first individual eclosed from each
 unique line and temperature exposure combination * DayFromFirst = The
 number of days an individual eclosed after the first individual of its
 given line and temperature exposure combination ##
 BE.Pup.Prob.MF.By.Day.csv and UZ.Pup.Prob.MF.By.Day.csv contain eclosion
 percentages by sex and column identifiers are as follows: * Day = The
 number of days after the first individual of its given line and
 temperature exposure combination * Prob = Survival model estimate of
 percentage of moths that had eclosed on that day * Sex = Sex of individual
 (F = female, M = male) * Line = Line of individual (E or Z line) ##
 BE.Pup.Prob.Temp.By.Day.csv and UZ.Prop.Temp.By.Day.csv contain eclosion
 percentages by temperature exposure and column identifiers are as follows:
 * Day = The number of days after the first individual of its given line
 and temperature exposure combination * Prob = Survival model estimate of
 percentage of moths that had eclosed on that day * TempID = Temperature
 exposure of individual using L for 23 degrees and H for 28 degrees at each
 life stage (LL = Control, HL = Larval, LH = Pupal, HH = ALL) * Line = Line
 of individual (E or Z line)