cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11313545.v1
title: Effect of endoparasites occurrence in sows from intensive production system

description: Abstract The goal of this study was to assess the effect of farm size (FS) and farrowing order (FO) on the occurrence of endoparasites eggs in commercial sows housed in maternity and gestation areas during the period from May to July 2014. Forty-three piglet production units were classified by FS: small (100 to 250 sows), medium (251 to 510 sows), large (511 to 1,000 sows) and very large (more than 1,000 sows). Sows were classified by FO: up to two, three to five or more than five parturitions. Faecal samples were processed using the simple flotation technique in a hypersaturated salt solution (30-35% NaCl). The results revealed that the overall prevalence of gastrointestinal endoparasites obtained in this study was 12.47%, in that 4.64% were positive for Ascaris suum, 0.56% for Trichuris suis and 8.27% for coccidia oocysts. The prevalence of endoparasites obtained for small and medium size farm, and for large and very large farm was 34.58% and 15.52%, respectively. In conclusion, the study shows that more than half of the farms were positive for A. suum and coccidia oocysts, but mainly for younger females. In general, sows with up to two parturitions and small farms showed a higher endoparasites percentage.
DOI: 10.6084/m9.figshare.11313524
title: Comparative study of the chemical composition, fatty acid profile, and nutritional quality of Lophiosilurus alexandri (Siluriformes: Pseudopimelodidae), a Brazilian carnivorous freshwater fish, grown in lotic, lentic, and aquaculture environments

description: ABSTRACT Lophiosilurus alexandri is a carnivorous freshwater fish endemic of São Francisco basin and an endangered species. In this study, we analysed the chemical composition (moisture, protein, ash and lipid), fatty acid profile, and nutritional quality (atherogenic index, thrombogenecity index, ratio between hypocholesterolemic and hypercholesterolemic fatty acids and ω6/ω3 = ratio) of L. alexandri in lotic (river), lentic (hydroelectric dams) environments, under natural fed, and in laboratory controlled conditions fed with commercial diets. Cultured and lentic fish had significantly higher lipid levels (1.5 and 1.9- fold, respectively) than lotic fish. Lentic L. alexandri had significantly higher eicosapentaenoic acid (EPA) levels (4×) than cultured or lotic L. alexandri. Docosahexaenoic acid (DHA) levels were the highest in lentic fish, followed by lotic fish. Lentic fish had greater proportion of fatty acid ω6/ω3 than lotic or cultured fish. The results of this study showed that L. alexandri is a lean fish (1-2% of total lipids) and that the environment has a great influence on the fatty acid profile. These results may be a reference for further studies, primarily as a source of information for conservation L. alexandri through restocking and the development of commercial projects of aquaculture.
DOI: 10.6084/m9.figshare.11313530
title: Upstream dam impacts on gilded catfish Brachyplatystoma rousseauxii (Siluriformes: Pimelodidae) in the Bolivian Amazon

description: ABSTRACT This paper explores the effects of two run-of-river dams (Jirau and Santo Antônio) built in cascade in the middle Madeira River (Brazil) on the interruption of long-distance migration routes of the gilded catfish (Brachyplatystoma rousseauxii). A participative monitoring system was set up to compare capture by commercial fishers approximately 1500 km upstream of the dams in Bolivia, before (1998-2007) and after (2015-2017) dam closure. A significant decrease in gilded catfish catches and in catch per unit effort was observed after dam closure, whereas no significant difference in mean weight was found. Back-estimation of age suggests that the few individuals remaining after dam closure in 2009 are a mixture of old homers that returned upstream before dam closure, and residents hatched after dam closure and trapped in the upper Madeira. Unless fishways in the Madeira River dams improve their efficiency, the gilded catfish might become rare and in danger of regional extinction in the upper Madeira basin in the next few years, negatively affecting river fisheries.
DOI: 10.6084/m9.figshare.11313521
title: Covariation in Precalculus Textbooks Tasks: a Case of Trigonometric Ratios

description: Abstract Variation learning has been recommended in several academic curricula around the world; however, the manner in which university textbooks carry out this purpose is unclear. We carried out an analysis of the textbook tasks in order to identify the way in which covariation is present in the trigonometry tasks. Content analysis tools were used to analyze the tasks of five university precalculus textbooks. The study shows a special emphasis on tasks that miss opportunities to mathematize the covariation. This article offers a typology of tasks and also suggests the possibility of reorganizing some of them, in order to promote covariation studies.
DOI: 10.6084/m9.figshare.11313530.v1
title: Upstream dam impacts on gilded catfish Brachyplatystoma rousseauxii (Siluriformes: Pimelodidae) in the Bolivian Amazon

description: ABSTRACT This paper explores the effects of two run-of-river dams (Jirau and Santo Antônio) built in cascade in the middle Madeira River (Brazil) on the interruption of long-distance migration routes of the gilded catfish (Brachyplatystoma rousseauxii). A participative monitoring system was set up to compare capture by commercial fishers approximately 1500 km upstream of the dams in Bolivia, before (1998-2007) and after (2015-2017) dam closure. A significant decrease in gilded catfish catches and in catch per unit effort was observed after dam closure, whereas no significant difference in mean weight was found. Back-estimation of age suggests that the few individuals remaining after dam closure in 2009 are a mixture of old homers that returned upstream before dam closure, and residents hatched after dam closure and trapped in the upper Madeira. Unless fishways in the Madeira River dams improve their efficiency, the gilded catfish might become rare and in danger of regional extinction in the upper Madeira basin in the next few years, negatively affecting river fisheries.
DOI: 10.6084/m9.figshare.11313512
title: VALUE FOCUSED PERFORMANCE INDICATORS FOR GRADUATE PROGRAMS

description: ABSTRACT Performance assessment is addressed in the literature as means for continuous improvement. The objective of this study was to identify the most valued performance criteria for a Graduate Program of Biotechnology in a University of Brazil through the Value Focused Thinking (VFT) approach. The choice of the VFT was due to the holistic vision provided by it and the focus on the identification of organizational values. Among the objectives found, it is possible to notice an expansion of the vision of performance that is more focused on publications regarding the regulatory body Coordination of Improvement of Higher Level Personnel (CAPES), and contemplating among others the quality in the student’s training as evidence of other stakeholders. The VFT approach met the established assumptions, efficiently allowing strategic, fundamental and media objectives to be identified, as well as measurable attributes to be used by the program to enable more efficient management.
DOI: 10.6084/m9.figshare.11313521.v1
title: Covariation in Precalculus Textbooks Tasks: a Case of Trigonometric Ratios

description: Abstract Variation learning has been recommended in several academic curricula around the world; however, the manner in which university textbooks carry out this purpose is unclear. We carried out an analysis of the textbook tasks in order to identify the way in which covariation is present in the trigonometry tasks. Content analysis tools were used to analyze the tasks of five university precalculus textbooks. The study shows a special emphasis on tasks that miss opportunities to mathematize the covariation. This article offers a typology of tasks and also suggests the possibility of reorganizing some of them, in order to promote covariation studies.
DOI: 10.6084/m9.figshare.11313524.v1
title: Comparative study of the chemical composition, fatty acid profile, and nutritional quality of Lophiosilurus alexandri (Siluriformes: Pseudopimelodidae), a Brazilian carnivorous freshwater fish, grown in lotic, lentic, and aquaculture environments

description: ABSTRACT Lophiosilurus alexandri is a carnivorous freshwater fish endemic of São Francisco basin and an endangered species. In this study, we analysed the chemical composition (moisture, protein, ash and lipid), fatty acid profile, and nutritional quality (atherogenic index, thrombogenecity index, ratio between hypocholesterolemic and hypercholesterolemic fatty acids and ω6/ω3 = ratio) of L. alexandri in lotic (river), lentic (hydroelectric dams) environments, under natural fed, and in laboratory controlled conditions fed with commercial diets. Cultured and lentic fish had significantly higher lipid levels (1.5 and 1.9- fold, respectively) than lotic fish. Lentic L. alexandri had significantly higher eicosapentaenoic acid (EPA) levels (4×) than cultured or lotic L. alexandri. Docosahexaenoic acid (DHA) levels were the highest in lentic fish, followed by lotic fish. Lentic fish had greater proportion of fatty acid ω6/ω3 than lotic or cultured fish. The results of this study showed that L. alexandri is a lean fish (1-2% of total lipids) and that the environment has a great influence on the fatty acid profile. These results may be a reference for further studies, primarily as a source of information for conservation L. alexandri through restocking and the development of commercial projects of aquaculture.
DOI: 10.6084/m9.figshare.11313512.v1
title: VALUE FOCUSED PERFORMANCE INDICATORS FOR GRADUATE PROGRAMS

description: ABSTRACT Performance assessment is addressed in the literature as means for continuous improvement. The objective of this study was to identify the most valued performance criteria for a Graduate Program of Biotechnology in a University of Brazil through the Value Focused Thinking (VFT) approach. The choice of the VFT was due to the holistic vision provided by it and the focus on the identification of organizational values. Among the objectives found, it is possible to notice an expansion of the vision of performance that is more focused on publications regarding the regulatory body Coordination of Improvement of Higher Level Personnel (CAPES), and contemplating among others the quality in the student’s training as evidence of other stakeholders. The VFT approach met the established assumptions, efficiently allowing strategic, fundamental and media objectives to be identified, as well as measurable attributes to be used by the program to enable more efficient management.
DOI: 10.6084/m9.figshare.11313494
title: The quantification of testicular cells during the postnatal development in two Caviomorph rodents: the guinea pig (Cavia porcellus) and the cutia (Dasyprocta agouti)

description: ABSTRACT The germinative, Sertoli and Leydig cells of two caviomorph rodents (Cavia porcellus and Dasyprocta agouti) were counted as well as the estimation of the total volume of the testis and the total volume of seminiferous tubules and interstitium in prepubertal, pubertal and adult animals. The number of spermatogonia, spermatocytes and spermatids cells increased during the pubertal phase in both rodents, notably the spermatid cells. The spermatocyte and spermatid slightly decreased in the adult of both rodents, but the increment in spermatogonia cells number was seen, mainly in cutias. The number of Sertoli cells increased in pubertal rodents, but in the adult the number reduced. Substantial number of Leydig cells was counted in pubertal and adult guinea pigs. In cutias, the number of Leydig cells increased in pubertal phase and decline in adults. The design-based stereological method has proven to be unbiased and reliable to be applied in reproduction studies.
DOI: 10.6084/m9.figshare.11313500
title: Leaf area estimation from linear measurements in different ages of Crotalaria juncea plants

description: ABSTRACT The goal of this study was to estimate the leaf area of Crotalaria juncea according to the linear dimensions of leaves from different ages. Two experiments were conducted with C. juncea cultivar IAC-KR1, in the 2014/2015 sowing seasons. At 59, 82, 102, 129 days after sowing (DAS) of the first and 61, 80, 92, 104 DAS of the second experiment, 500 leaves were collected, totaling 4,000 leaves. In each leaf, the linear dimensions were measured (length, width, length/width ratio and length × width product) and the specific leaf area was determined through Digimizer and Sigma Scan Pro software, after scanning images. Then, 3,200 leaves were randomly separated to generate mathematical models of leaf area (Y) in function of linear dimension (x), and 800 leaves for the models validation. In C. juncea, the leaf areas determined by Digimizer and Sigma Scan Pro software are identical. The estimation models of leaf area as a function of length × width product showed superior adjustments to those obtained based on the evaluation of only one linear dimension. The linear model Ŷ=0.7390x (R2=0.9849) of the real leaf area (Y) as a function of length × width product (x) is adequate to estimate the C. juncea leaf area.
DOI: 10.6084/m9.figshare.11313491
title: An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef

description: Abstract The aim of this study is to optimize a rapid and specific method for detection of E. coli O157:H7 (EHEC) from food samples with high background flora using an immunomagnetic separation-real-time polymerase chain reaction assay (IMS-RTiPCR). For this, EHEC cells were recovered from raw milk and ground meat samples that were artificially contaminated to the final concentrations of 101, 103 and 105 cfu EHEC/mL or g, after a non-selective pre-enrichment at 35 °C for 8 h following either with or without capturing by micro-sized beads. Then, EHEC cells were identified by RTiPCR. The study was also carried out without any enrichment to evaluate the enrichment efficiency of the assay. By comparing the assay with and without pre-enrichment, we showed that even the usage of specific micro-sized beads did not improve EHEC detection in raw milk and ground beef samples and so gave false negative results unless an 8-h pre-enrichment was applied. Besides, the assay can be completed in less than 9 h with a minimum detection limit of 103 cfu EHEC/mL or g in foods and hence was found to be simple routine-based method that exhibits great potential for implementing as a rapid screening of EHEC in microbial tests as compared to existing techniques.
DOI: 10.6084/m9.figshare.11313494.v1
title: The quantification of testicular cells during the postnatal development in two Caviomorph rodents: the guinea pig (Cavia porcellus) and the cutia (Dasyprocta agouti)

description: ABSTRACT The germinative, Sertoli and Leydig cells of two caviomorph rodents (Cavia porcellus and Dasyprocta agouti) were counted as well as the estimation of the total volume of the testis and the total volume of seminiferous tubules and interstitium in prepubertal, pubertal and adult animals. The number of spermatogonia, spermatocytes and spermatids cells increased during the pubertal phase in both rodents, notably the spermatid cells. The spermatocyte and spermatid slightly decreased in the adult of both rodents, but the increment in spermatogonia cells number was seen, mainly in cutias. The number of Sertoli cells increased in pubertal rodents, but in the adult the number reduced. Substantial number of Leydig cells was counted in pubertal and adult guinea pigs. In cutias, the number of Leydig cells increased in pubertal phase and decline in adults. The design-based stereological method has proven to be unbiased and reliable to be applied in reproduction studies.
DOI: 10.6084/m9.figshare.11313488
title: Biochemical and Molecular Mechanisms of Glucose Uptake Stimulated by Physical Exercise in Insulin Resistance State: Role of Inflammation

description: Abstract Obesity associated with systemic inflammation induces insulin resistance (IR), with consequent chronic hyperglycemia. A series of reactions are involved in this process, including increased release of proinflammatory cytokines, and activation of c-Jun N-terminal kinase (JNK), nuclear factor-kappa B (NF-κB) and toll-like receptor 4 (TLR4) receptors. Among the therapeutic tools available nowadays, physical exercise (PE) has a known hypoglycemic effect explained by complex molecular mechanisms, including an increase in insulin receptor phosphorylation, in AMP-activated protein kinase (AMPK) activity, in the Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) pathway, with subsequent activation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), Rac1, TBC1 domain family member 1 and 4 (TBC1D1 and TBC1D4), in addition to a variety of signaling molecules, such as GTPases, Rab and soluble N-ethylmaleimide-sensitive factor attached protein receptor (SNARE) proteins. These pathways promote greater translocation of GLUT4 and consequent glucose uptake by the skeletal muscle. Phosphoinositide-dependent kinase (PDK), atypical protein kinase C (aPKC) and some of its isoforms, such as PKC-iota/lambda also seem to play a fundamental role in the transport of glucose. In this sense, the association between autophagy and exercise has also demonstrated a relevant role in the uptake of muscle glucose. Insulin, in turn, uses a phosphoinositide 3-kinase (PI3K)-dependent mechanism, while exercise signal may be triggered by the release of calcium from the sarcoplasmic reticulum. The objective of this review is to describe the main molecular mechanisms of IR and the relationship between PE and glucose uptake.
DOI: 10.6084/m9.figshare.11313500.v1
title: Leaf area estimation from linear measurements in different ages of Crotalaria juncea plants

description: ABSTRACT The goal of this study was to estimate the leaf area of Crotalaria juncea according to the linear dimensions of leaves from different ages. Two experiments were conducted with C. juncea cultivar IAC-KR1, in the 2014/2015 sowing seasons. At 59, 82, 102, 129 days after sowing (DAS) of the first and 61, 80, 92, 104 DAS of the second experiment, 500 leaves were collected, totaling 4,000 leaves. In each leaf, the linear dimensions were measured (length, width, length/width ratio and length × width product) and the specific leaf area was determined through Digimizer and Sigma Scan Pro software, after scanning images. Then, 3,200 leaves were randomly separated to generate mathematical models of leaf area (Y) in function of linear dimension (x), and 800 leaves for the models validation. In C. juncea, the leaf areas determined by Digimizer and Sigma Scan Pro software are identical. The estimation models of leaf area as a function of length × width product showed superior adjustments to those obtained based on the evaluation of only one linear dimension. The linear model Ŷ=0.7390x (R2=0.9849) of the real leaf area (Y) as a function of length × width product (x) is adequate to estimate the C. juncea leaf area.
DOI: 10.6084/m9.figshare.11313482
title: Assessment of the impacts of the consumers’ awareness of organic food on consumption behavior

description: Abstract Consumer demand for organic foods has increased significantly in recent years in the world. Consumers are influenced by various internal/external factors when purchasing food products. The aim of this study is to determine the effects of knowledge variable on consumers’ purchasing or consuming behavior for organic foods together with other factors in Turkey (Hatay City). For this purpose, the effects of consumers’ organic product knowledge levels are examined within the frame of Theory of Planned Behavior. The data of 431 consumers analyzed in the model were collected in 2017 with a 21 scale questionnaire and analyzed with Structural Equation Model. According to the results of the model analysis, a significant part of the variance in consumers' organic food demand (61%) was explained by the organic food knowledge variable. Reducing consumption obstacles, along with efforts to increase the organic food knowledge of consumers, will increase organic demand. While trying to increase the supply of organic products especially in regions where consumers are concentrated, the food production sector should also focus on informing, raising awareness and promoting. The actual buying behavior in organic food products should be examined in more detail in terms of motivation sources and obstacles.
DOI: 10.6084/m9.figshare.11313485
title: First record of Scybalocanthon nigriceps (Harold, 1868) (Coleoptera: Scarabaeidae: Scarabaeinae) in Rio Grande do Sul state, southern Brazil

description: ABSTRACT The dung beetle, Scybalocanthon nigriceps (Harold, 1868), is recorded in Rio Grande do Sul state, Brazil, for the first time, at the Moreno Fortes Biological Reserve, municipality of Dois Irmãos das Missões, northwest region of the state, expanding the area of occurrence and distribution of this species in the country.
DOI: 10.6084/m9.figshare.11313491.v1
title: An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef

description: Abstract The aim of this study is to optimize a rapid and specific method for detection of E. coli O157:H7 (EHEC) from food samples with high background flora using an immunomagnetic separation-real-time polymerase chain reaction assay (IMS-RTiPCR). For this, EHEC cells were recovered from raw milk and ground meat samples that were artificially contaminated to the final concentrations of 101, 103 and 105 cfu EHEC/mL or g, after a non-selective pre-enrichment at 35 °C for 8 h following either with or without capturing by micro-sized beads. Then, EHEC cells were identified by RTiPCR. The study was also carried out without any enrichment to evaluate the enrichment efficiency of the assay. By comparing the assay with and without pre-enrichment, we showed that even the usage of specific micro-sized beads did not improve EHEC detection in raw milk and ground beef samples and so gave false negative results unless an 8-h pre-enrichment was applied. Besides, the assay can be completed in less than 9 h with a minimum detection limit of 103 cfu EHEC/mL or g in foods and hence was found to be simple routine-based method that exhibits great potential for implementing as a rapid screening of EHEC in microbial tests as compared to existing techniques.
DOI: 10.6084/m9.figshare.11313479
title: Post-harvest conservation of Passiflora alata fruits under ambient and refrigerated condition

description: Abstract Brazil is the world's largest producer of passion fruit, a perishable fruit with reduced post-harvest shelf life, which limits its commercialization. For sweet passion fruit, Passiflora alata, there is little information about its postharvest conservation and shelf life under different storage conditions. Thus, the objective of the present study was to determine the post-harvest conservation of P. alata fruits packed on styrofoam trays rapped with 10 μm PVC film and HDPE 30 μm thick, coated with 18% carnauba wax and without any packaging; stored under ambient conditions (25 °C and 70% relative humidity) and refrigerated (10 °C and 90% relative humidity) for 14 days. The best results were obtained with P. alata fruits packaged on styrofoam trays rapped with 10 μm PVC flexible film and kept under refrigeration in a cold room at a temperature of 10 °C and relative humidity between 85% and 90% for a period of 10 to 14 days. Fruits of P. alata , for commercialization “in natura”, kept under ambient conditions, should not be packaged and have a shelf life of up to seven days.