DOI: 10.5061/dryad.p8cz8wb15
title: Data from: Ledebouria mavelii (Asparagaceae), a new species from Palakkad gap, Kerala, India

description: Ledebouria mavelii, a new species from Asparagaceae, is described and
 compared with L. hyderabadensis, L. karnatakensis, and L. revoluta. L.
 mavelii is confined to Palakkad District, Kerala. Detailed description,
 distribution, phenology, photographs, and key to Ledebouria species in
 India are provided. A phylogenetic analysis was conducted using sequences
 of two plastid genes, the maturase-coding gene (matK) and the large
 subunit of ribulose 1,5-bisphosphate carboxylase-coding gene (rbcL) as DNA
 markers. The phylogenetic trees constructed from matK and rbcL data
 successfully distinguished each species from one another.
description: The sequence data was obtained thorugh sanger sequencing of the
 sample of the plants collected form the type location
description: # Ledebouria mavelii (Asparagaceae), a new species from Palakkad gap,
 Kerala, India
 [https://doi.org/10.5061/dryad.p8cz8wb15](https://doi.org/10.5061/dryad.p8cz8wb15) ## Description of the data and file structure We conducted Sanger sequencing on **Ledebouri sp. nov.**, a newly discovered plant species from Palakkad, to obtain its DNA sequence data. Leaf samples were collected from specimens in their natural habitat. Genomic DNA was extracted using standard molecular biology protocols. Specific gene regions were amplified via PCR and sequenced to aid in the taxonomic classification and phylogenetic analysis of this new species. ### Files and variables #### File: SR3781-MATKF\_E07.fsta **Description:** Maturase Kinase - Forward of Ledebouria sp nov #### File: SR3781-MATKR\_E08.fsta **Description:** Maturase Kinase - Reverse of Ledebouria sp nov #### File: SR3781-RBCLF\_F07.fsta **Description:** rbcL forwared of Ledebouria sp nov #### File: SR3781-RBCLR\_F08.fsta **Description:** rbcL reverse of Ledebouria sp nov ## Code/software The dataset consists of four FASTA files containing DNA sequences obtained from Sanger sequencing of *Ledebouri* sp. nov. These files can be accessed and analyzed using the following free or open-source software: 1. **Text Editors (for Viewing):** * **Notepad++** (Version 7.9.5) * **Description**: A free source code editor that supports several languages and is suitable for viewing and editing plain text files, including FASTA files. * **Download Link**: [Notepad++](https://notepad-plus-plus.org/) * **Dependencies**: Windows OS. * **Sublime Text** (Version 3.2.2) * **Description**: A sophisticated text editor for code, markup, and prose. * **Download Link**: [Sublime Text](https://www.sublimetext.com/) * **Dependencies**: Cross-platform (Windows, macOS, Linux). 2. **Bioinformatics Software (for Analysis):** * **MEGA X** (Version 10.2.6) * **Description**: MEGA X (Molecular Evolutionary Genetics Analysis) is an integrated tool for conducting sequence alignment and phylogenetic analysis. * **Download Link**: [MEGA Software](https://www.megasoftware.net/) * **Dependencies**: None. * **BioEdit** (Version 7.2.6) * **Description**: A user-friendly biological sequence alignment editor with numerous functions for sequence manipulation and analysis. * **Download Link**: BioEdit * **Dependencies**: Windows OS. * **UGENE** (Version 38.1) * **Description**: An open-source cross-platform bioinformatics software for molecular biologists. * **Download Link**: [UGENE](https://ugene.net/) * **Dependencies**: None. ## Access information Other publicly accessible locations of the data: * n/a Data was derived from the following sources: *