DOI: 10.22002/w0y1q-gyv74
title: Lp02 ΔlspDE

description: Raw data files of Lp02 ΔlspDE
description: <strong>Tilt Series Date:</strong> 2017-03-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Nicholas Cianciotto at Northwestern University Medical School Data collected and processed by Debnath Ghosal</p>
<strong>Sample Preparation:</strong> 4 grids were frozen for this strain at OD= 3 Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-02-40/rawdata/</p>Lg_DeltaDE_201703020001.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
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        Lg_DeltaDE_201703020001_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
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        Lg_DeltaDE_201703020001_peak.mod,  61.7 kB  
        
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        Lg_DeltaDE_201703020001_fid.tlt,  648.0 B  
        
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        Lg_DeltaDE_201703020001.tlt,  648.0 B  
        
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        Lg_DeltaDE_201703020001.ali,  577.3 MB  
        
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