DOI: 10.6084/m9.figshare.14130065
title: Drought adaptation modifies soil respiration and microbial community responses to phytohormones

description: Datasets used in: Sayer EJ, Crawford JA, Edgerley J, Askew AP, Hahn CZ, Whitlock R and Dodd IC (2021) Adaptation to chronic drought modifies soil microbial community responses to phytohormones. <i>Communications Biology</i> MS COMMSBIO-20-2294A <br> <b>File 1:</b> SayerEtAl2021_CommsBiol_SoilResp.csv <br> Respiration rates (CO<sub>2</sub> efflux) following phytohormone additions at three molar concentrations or a standard root exudate solution, applied to soils from long-term climate change treatments at the Buxton Climate Change Impacts Study, UK. Respiration rates were measured using the Microresp method during 6-h incubations. <br> Column headings: Block -&gt; replicate block, determined by original experimental design in the field Climate.Plot -&gt; climate treatment applied to field plots RE.add -&gt; addition of standard root exudate solution (RE) or no addition (noRE) Hormone.Trt -&gt; phytohormone treatment applied to the soils, where ABA is abscisic acid, ACC is 1-aminocyclopropane-1-carboxylic acid and JA is jasmonic acid Concentration-&gt; molar concentration of the phytohormone solutions applied to soils, where 0M is a procedural control (deionised water) CO2efflux-&gt;total soil respiration in micrograms (ug) C <br> <b>File 2:</b> SayerEtAl2021_CommsBiol_PLFAs.csv Soil properties and soil microbial biomarker groups in field soils (inc.Time = 0) and soil microbial biomarker groups after 24h incubation with phytohormone additions at 1M concentration; soils were sampled from long-term climate change treatments at the Buxton Climate Change Impacts Study, UK; biomarker functional groups were determined by phospholipid fatty acid analysis and are given as relative abundances. <br> Column headings: Block -&gt; replicate block, determined by original experimental design in the field Climate.Plot -&gt; climate treatment applied to field plots Hormone.Trt -&gt; phytohormone treatment applied to the soils at 1M concentration, where ABA is abscisic acid, ACC is 1-aminocyclopropane-1-carboxylic acid and JA is jasmonic acid; CT is field soils before incubation and H2O is a procedural control (incubated with deionised water only). Inc.Time -&gt; incubation time in hours, where 0 is field soils (pre-incubation) Total.Biomass -&gt; total PLFA biomass in nM/g soil AM.Fungi-&gt; arbuscular mycorrhizal fungi (% relative abundance) Gram.Negative -&gt; Gram negative bacteria (% relative abundance) Eukaryote-&gt; Eukaryotes (% relative abundance) Fungi -&gt; saprophytic fungi (% relative abundance) Gram.Positive -&gt; Gram positive bacteria (% relative abundance) Actinomycetes -&gt; Actinomycetes (% relative abundance) FBratio -&gt; Fungal to bacterial ratio nMfungi -&gt; saprophytic fungal biomass in nM/g soil nMbacteria -&gt; bacterial biomass in nM/g soil Gram.Ratio -&gt; ratio of Gram positive to Gram negative bacteria SWC.percent -&gt; soil water content (%) at the time of sampling ExtrC.mg.g -&gt; K2SO4-extractable soil carbon (mg/g dry weight) ExtrN.mg.g-&gt; K2SO4-extractable soil nitrogen (mg/g dry weight) <br> <br>