cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.1594/pangaea.920269
title: Seawater carbonate chemistry and mass and fine-scale morphological changes of Coccolith Gephyrocapsa oceanica

description: Coccolithophores have been extensively studied to understand the environmental control on calcification in a key biological group influencing the alkalinity of seawater. Previous studies have established that bulk calcification scales with cell division rates under a wide range of pH conditions. Yet, the fine scale ultrastructural changes of the coccoliths and therefore the pH‐sensitive underlying mechanisms altering biomineralization of the coccoliths remain largely under‐constrained. Using circularly polarized light and high resolution microscopy, we have generated mass estimates of cultured Gephyrocapsa oceanica coccoliths grown in medium with pH values ranging from 7.4 to 9.0. These mass estimates representing a bulk calcification response were related to the morphological changes within the coccoliths. From optimal (pH 8.6) down to pH 7.4 conditions, we have observed that impaired cell growth and lower calcite quota are accompanied by a 35% decrease in mean coccolith mass. The data further show that seawater acidification does not homogenously affect calcification of the coccoliths, as a clear reduction in the breadth of the tube (a structure surrounding the central area of the coccoliths) was detected, whereas all other ultrastructural components were far less impacted. We discuss this specific sensitivity to acidification as the consequence of the altered interaction of the acidic polysaccharides used for biomineralization and ambient concentration of protons released by calcification that substantially modify the growth patterns, the morphology and ultimately the mass of the coccoliths.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.
DOI: 10.1594/pangaea.919708
title: Seawater carbonate chemistry and total wet weight, metabolically active tissue, ingestion rate and oocyte diameter of Adult Mussels Mytilus chilensis

description: The effect of CO2-driven ocean acidification (OA) on marine biota has been extensively studied mostly on a single stage of the life cycle. However, the cumulative and population-level response to this global stressor may be biased due to transgenerational effects and their impacts on physiological plasticity. In this study, we exposed adult mussels Mytilus chilensis undergoing gametogenesis to two pCO2 levels (550 and 1200 μatm) for 16 weeks, aiming to understand if prolonged exposure of reproductive individuals to OA can affect the performance of their offspring, which, in turn, were reared under multiple stressors (pCO2, temperature, and dissolved cadmium). Our results indicate dependence between the level of pCO2 of the broodstock (i.e., parental effect) and the performance of larval stages in terms of growth and physiological rates, as a single effect of temperature. While main effects of pCO2 and cadmium were observed for larval growth and ingestion rates, respectively, the combined exposure to stressors had antagonistic effects. Moreover, we found a suppression of feeding activity in post-spawning broodstock upon high pCO2 conditions. Nevertheless, this observation was not reflected in the final weight of the broodstock and oocyte diameter. Due to the ecological and socioeconomic importance of mussels' species around the globe, the potential implications of maternal effects for the physiology, survival, and recruitment of larvae under combined global-change stressors warrant further investigation.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-06-12.
DOI: 10.1594/pangaea.927463
title: Geochemistry of Puscizna Mala peat core from Poland

description: Geochemistry data of a high-resolution peat core from the Past Global Changes - Carbon in Peat on EArth through Time (PAGES_C-PEAT) Project.
DOI: 10.1594/pangaea.929313
title: Calibrated ages of Puscizna Mala peat core from Poland

description: Calibrated ages of a high-resolution peat core from the Past Global Changes - Carbon in Peat on EArth through Time (PAGES_C-PEAT) Project.
DOI: 10.1594/pangaea.929683
title: Abundance of benthic peracarids sampled in the Atlantic Sector of the Southern Ocean and Weddell Sea during expeditions JR15005, JR275, JR17003a, PS118

description: Peracarid abundance and composition were studied in the Atlantic Sector of the Southern Ocean and the Weddell Sea at a depth range of 403-2021 m. Samples were collected using an epibenthic sledge (EBS) during expeditions on board the RRS James Clarke Ross in the Filchner Trough (JR275), the South Orkney Islands (JR15005), the Prince Gustav Channel (JR17003a) and on board the RV Polarstern in the Eastern Antarctic Peninsula (PS118). Expeditions took place in February-March 2012, 2016, 2018 and 2019 respectively. Since the trawling distance between stations was not always the same, in order to make data comparable between different stations, numbers of individuals were standardized to 1000 m haul distances.In total 64766 peracarids were found and sorted into five different orders (Amphipoda, Cumacea, Isopoda, Mysidacea and Tanaidacea). Amphipods were the most abundant group representing the 32% of the total abundances. The Filchner Trough had the highest abundance of peracarids, while the South Orkney Islands showed the lowest abundance compared to other areas.
DOI: 10.1594/pangaea.930555
title: Seawater carbonate chemistry and growth of Prochlorococcus and Synechococcus strains

description: Anthropogenic CO2 emissions are projected to lower the pH of the ocean 0.3 units by 2100. Previous studies suggested that Prochlorococcus and Synechococcus, the numerically dominant phytoplankton in the oceans, have different responses to elevated CO2 that may result in a dramatic shift in their relative abundances in future oceans. Here we showed that the exponential growth rates of these two genera respond to future CO2 conditions in a manner similar to other cyanobacteria, but Prochlorococcus strains had significantly lower realized growth rates under elevated CO2 regimes due to poor survival after exposure to fresh culture media. Despite this, a Synechococcus strain was unable to outcompete a Prochlorococcus strain in co‐culture at elevated CO2. Under these conditions, Prochlorococcus' poor response to elevated CO2 disappeared, and Prochlorococcus' relative fitness showed negative frequency dependence, with both competitors having significant fitness advantages when initially rare. These experiments suggested that the two strains should be able to co‐exist indefinitely in co‐culture despite sharing nearly identical nutritional requirements. We speculate that negative frequency dependence exists due to reductive Black Queen evolution that has resulted in a passively mutualistic relationship analogous to that connecting Prochlorococcus with the “helper” heterotrophic microbes in its environment.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-19.
DOI: 10.1594/pangaea.934120
title: Age determination of Puscizna Mala peat core from Poland

description: 14C and 210Pb age determination of a high-resolution peat core from the Past Global Changes - Carbon in Peat on EArth through Time (PAGES_C-PEAT) Project
DOI: 10.1594/pangaea.936123
title: Heat flow data at station HF2020 during RV SONNE cruise SO278

description: Results of heat flow station HF2020, penetrations 1 - 10, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936230
title: Heat flow data at station HF2021 during RV SONNE cruise SO278

description: Station HF2021 pentration 1 - 11 temperature-time raw data in ASCII Format. Temperature in Milli Kelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2021, penetrations 1 - 13, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set.
DOI: 10.1594/pangaea.936437
title: Heat flow data at station HF2027 during RV SONNE cruise SO278

description: Heat flow station HF2027 penetration 1 – 12, temperature-time raw data in ASCII Format. Temperature in Millikelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2027, penetrations 1 - 12, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936438
title: Heat flow data at station HF2028 during RV SONNE cruise SO278

description: Heat flow station HF2028 penetration 1 – 10, temperature-time raw data in ASCII Format. Temperature in Millikelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2028, penetrations 1 - 10, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936439
title: Heat flow data at station HF2029 during RV SONNE cruise SO278

description: Heat flow station HF2029 penetration 1 – 12, temperature-time raw data in ASCII Format. Temperature in Millikelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2029, penetrations 1 - 12, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936235
title: Heat flow data at station HF2022 during RV SONNE cruise SO278

description: Heat flow station HF2022 pentration 1 - 6 temperature-time raw data in ASCII Format. Temperature in Milli Kelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2022, penetrations 1 - 6, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936256
title: Heat flow data at station HF2023 during RV SONNE cruise SO278

description: Heat flow station HF2023 penetration 1 - 8, temperature-time raw data in ASCII Format. Temperature in milli Kelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2023, penetrations 1 - 8, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936274
title: Heat flow data at station HF2024 during RV SONNE cruise SO278

description: Heat flow station HF2024 penetration 1 – 10, temperature-time raw data in ASCII Format. Temperature in milli Kelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2024, penetrations 1 - 10, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936341
title: Heat flow data at station HF2025 during RV SONNE cruise SO278

description: Heat flow station HF2025 penetration 1 – 8, temperature-time raw data in ASCII Format. Temperature in milli Kelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2025, penetrations 1 - 8, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.936345
title: Heat flow data at station HF2026 during RV SONNE cruise SO278

description: Heat flow station HF2026 penetration 1 – 11, temperature-time raw data in ASCII Format. Temperature in milli Kelvin (mK) and time in GMT, 1 second sample interval. Results of heat flow station HF2026, penetrations 1 - 11, results of in-situ temperatures and thermal conductivities in depth dependent order. Temperature is in Degree Celsius (°C), thermal conductivity in (W/m*K). Thermal conductivity can be measured or set. Data presentation is ASCII (.dat files) and graphic (.PDF files).
DOI: 10.1594/pangaea.946416
title: Processed crosstab showing identified DOM molecular formulae and corresponding relative intensities of mesocosm samples from sediment core GeoB 19339-1
DOI: 10.1594/pangaea.946418
title: Calculated intensity-weighted parameters and relative assignments to compound groups of FT-MS measurements of DOM for each sample from sediment core GeoB 19339-1

description: Based on molecular formulae assigned to ESI-FT-ICR-MS peaks (Electrospray ionization Fourier-transform ion cyclotron resonance mass spectrometry).Compound group assignments after Seidel et al. 2014; https://doi.org/10.1016/j.gca.2014.05.038
DOI: 10.1594/pangaea.946419
title: Excitation and emission of PARAFAC model loadings for a four component model
DOI: 10.1594/pangaea.949041
title: Fluid geochemical data from batch experiments simulating hydrothermal troctolite alteration at 300 and 400°C (40 MPa)

description: *Values were modelled analogue to those for samples taken at elevated temperatures for visual comparison.
DOI: 10.1594/pangaea.949046
title: Mineral chemistry data from batch experiments simulating hydrothermal troctolite alteration at 300°C (40 MPa)

description: 1XMg = Mg / (Mg + Fe)2XCa = Ca / (Ca + Na) *Initial characterization of the reactants
DOI: 10.1594/pangaea.949054
title: Mineral chemistry data from batch experiments simulating hydrothermal troctolite alteration at 400°C (40 MPa)

description: *Iron for andradite-grossular solid solutions was calculated as ferric Fe3+1Mg = Mg / (Mg + Fe) 2XCa = Ca / (Ca + Na) 3XFe = Fe / (Fe + Al)
DOI: 10.1594/pangaea.949079
title: Mass balance and comparative thermodynamic reaction path data for batch experiments simulating hydrothermal troctolite alteration at 300 and 400°C (40 MPa)

description: *Exact compositions derived from EMP based WDS analyses were used in the regression algorithm**Resulting mineral proportions for a reaction turnover of 77.49% (300 °C) and 52.23% (400 °C) by weight - Modelling was conducted at 50 MPa1Sum of serpentine and chlorite equals the fraction derived by TG 2Molar weight from the 300 °C run was used to calculate the mole equivalent for the model run3Brucite fraction equals the amount derived by TG plus 1 wt.% dissolved fraction4Magnetite determined via AGFM
DOI: 10.1594/pangaea.950850
title: Incubation chamber volume and algal biomass, surface area and epiphyte biomass of Fucus vesiculosus specimen sampled at approx 1.5 m depth in the coastal Baltic Sea at Tvärminne Zoological Station, Hanko, Finland, in August 2020

description: For the algae incubations on August 18, 2020, transparent gas impermeable plastic vacuum bags (Packing24, PA/PE 20/70, 90my) with a single F. vesiculosus thallus, were closed with rubber bands around PVC end cap fitting of 12 cm diameter and attached to a bottom-anchored line at ~1 m depth. Thalli of ~50 cm length were detached close to the base by cutting the few millimeters thick, old stipe. Half of the incubations were covered with black bags to simulate dark conditions. Immediately prior to starting the incubations, water was sampled from the ambient water to determine initial conditions. At the end of the incubation, all algal tissue was collected. Water from within chambers was collected for analyses and the total volume measured. In the laboratory, the algal thallus was spread out across a cutting mat with a grid for surface area estimation. Epiphytes and epifauna were scraped off thalli using a razor blade, and collected separately from the algal biomass. All biomass was frozen and subsequently freeze-dried for 24 hours, and weighed to determine dry weight.
DOI: 10.1594/pangaea.950857
title: Above- and belowground biomass, number of blades and surface area of Zostera marina specimen sampled at approx 3 m depth in the coastal Baltic Sea at Tvärminne Zoological Station, Hanko, Finland, in August 2020

description: For seagrass incubations, August 5, 2020, seagrass shoots were enclosed in transparent gas impermeable plastic vacuum bags (Packing24, PA/PE 20/70, 90my) with approx. 6 L of the surrounding water. The bags were sealed onto the PVC tubes of ~12 cm diameter installed in the sediment one day earlier. Half of the incubations were covered with black bags to simulate dark conditions. Immediately prior to starting the incubations, water was sampled from the ambient water to determine initial conditions. At the end of the incubation, all seagrass tissue, above- and below-ground, was collected. Water from within chambers was collected for analyses. In the laboratory, the length and width of each seagrass shoot was measured. Epiphytes and epifauna were scraped off seagrass shoots using a razor blade, and collected separately from the seagrass biomass. All biomass was frozen and subsequently freeze-dried for 24 hours, and weighed to determine dry weight.
DOI: 10.1594/pangaea.950862
title: Oxygen concentrations, dissolved organic carbon concentrations, total dissolved nitrogen concentrations, absorbance and fluorescence of 0.7 µm-filtered seawater from incubation experiments with Fucus vesiculosus and Zostera marina specimen

description: Water column samples from seagrass incubations were collected into acid washed single use, sterile, 60 mL syringes (Thermo Fisher Scientific, USA) via water column ports on the PVC tubes. Water column samples from algae incubations were collected into acid washed 60 mL syringes after opening incubation bags on board. Water column samples were filtered immediately through a precombusted 45 mm diameter glass microfiber GF/F filter (Whatman, UK). For each sample, 30 mL were pushed through the filter for rinsing before starting collection. To determine the concentration of dissolved organic carbon (DOC) and total dissolved nitrogen (TDN), 15 mL sample were filtered into a precombusted 24 mL glass vial containing 60 µL 25% hydrochloric acid and the vial sealed with an acid washed teflon-lined cap. Another 10 mL of sample were filtered into a precombusted 12 mL glass vial and the vial sealed with an acid washed teflon-lined cap for colored dissolved organic matter (CDOM) and fluorescent dissolved organic matter (FDOM) analysis. Subsamples for DOC were frozen at -20°C and samples for CDOM/FDOM were stored at 4°C until analysis within two weeks. Optical FireSting O2 sensors (Pyroscience GmbH, Germany) were used to determine oxygen concentrations in water samples. Calibration of the sensors was achieved using MilliQ water. Oxygen concentrations were measured in 600 mL subsamples from water column within two hours of sample collection. For algae incubations, oxygen concentrations could also be determined in water from incubations without prior filtration. For DOC and TDN concentrations, the samples were analyzed using a Shimadzu TOC-VCPH-analyzer and an autoanalyzer (Aquakem 250). CDOM absorption was measured using a Shimadzu 2401PC spectrophotometer with 5 cm quartz cuvette over the spectral range from 200 to 800 nm with 1 nm resolution. Ultrapure water was used as the blank for all samples. Excitation-emission matrices (EEMs) of FDOM were measured with a Varian Cary Eclipse fluorometer (Agilent). Processing of the EEMs was done using the eemR package for R software. A blank sample of ultrapure water was subtracted from the EEMs, and the Rayleigh and Raman scattering bands were removed from the spectra after calibration. EEMs were calibrated by normalizing to the area under the Raman water scatter peak (excitation wavelength of 350 nm) of an ultrapure water sample run on the same session as the samples, and were corrected for inner filter effects with absorbance spectra.
DOI: 10.1594/pangaea.950868
title: Glycan-specific analyses conducted on water samples from incubation experiments with seagrass and algae sampled from Tvärminne Zoological Station, Hanko Finland, in August 2020

description: This data was collected to investigate dissolved organic molecules that are released by seagrass and brown algae within the framework of an Assemble Plus Transnational Access project called Contributions of organic carbon from vegetated coastal ecosystems. The field experiments and water sample analyses were conducted in August 2020 at Tvärminne Zoological Station, Hanko Finland. The binary file glycan_data_ELISA contains results from antibody staining of seawater samples from algae incubations. Two fucoidan-targeting monoclonal antibodies were used, namely BAM1 and BAM2. Signal intensity indicating recognition of the substrate target in a sample by the antibody is given as relative intensity normalized to 1. The binary file glycan_data_HPAEC-PAD contains unprocessed results from high performance anion exchange chromatography which was used to quantify monosaccharides. Glycans in the samples were degraded to monosaccharides unspecifically using acid hydrolysis, or in a targeted manner using an enzyme cocktail specific to fucoidan. The binary file glycan_data_Fig2c contains data on fucoidan extraction from seawater using anion exchange chromatography. During the chromatography, two consecutive elution steps first with 2 M ammonium bicarbonate followed by 5 M sodium chloride were performed. The two eluted fractions were analyzed separately and results for monosaccharide content are given in this table. These glycan analyses were performed in 2020 and 2021 at the Max-Planck-Institute for marine microbiology in Bremen, Germany.