cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/tzb3e-hfm27
title: nanowire+type4pili+Chemo

description: Raw data files of nanowire+type4pili+Chemo
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Sample Preparation:</strong> This sample is from the chemostat. Nothing has been mutated. So its a wt chemostat sample</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-05-11-5/rawdata/</p>051115_nano0005.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-05-11-5/rawdata/051115_nano0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        051115_nano0005_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-05-11-5/3dimage_56338/051115_nano0005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pyby4-qsq34
title: Cos7 cells

description: Synchronized Cos7 cells, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2015-01-20</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Cos7</p>
<strong>Strain:</strong> Cos7 cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Sample Preparation:</strong> Synchronized Cos7 cells with siRNA NUP 153 and Thymidine 2mM</p>
  rra2015-01-20-1_slicer12707.jpg: Microtubules tightly packed in midbody rra2015-01-20-1_slicer12710.jpg: Microtubules packed in midbody rra2015-01-20-1_slicer12711.jpg: microtubule end rra2015-01-20-1_slicer12722.jpg: entering endosome?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-20-1/rawdata/</p>cos100001.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-20-1/rawdata/cos100001.mrc"
        > <i class="download icon"></i> Download </a></p>

        cos100001_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-20-1/3dimage_51128/cos100001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qdt2j-5sy78
title: Starving E-coli (cbpA- knockout)

description: keywords: late stage stationary phase, starvation, MC105 cbpA-
description: <strong>Tilt Series Date:</strong> 2015-08-12</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strains prepared by James Haycocks in the David Grainger Lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in LB broth at 33 degrees while shaken at 400 rpm for 11 days</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-08-12-16/rawdata/</p>cbpAminustomogramAugust0006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-08-12-16/rawdata/cbpAminustomogramAugust0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        cbpAminustomogramAugust0006_part121_43.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-08-12-16/3dimage_60391/cbpAminustomogramAugust0006_part121_43.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.d5ufgq
title: Occurrence Download

description: A dataset containing 87 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster horizontalis",
 "Country is Luxembourg",
 "HasCoordinate is true"
 ]
}

 The dataset includes 87 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002175-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/pvbky-ppz31
title: TM7 tomogram data

description: TM7  human oral cavity
description: <strong>Tilt Series Date:</strong> 2017-05-07</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Candidatus Saccharibacteria</p>
<strong>Strain:</strong> TM7</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-53.0°, 53.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Wenyuan Shi</p>
<strong>Sample Preparation:</strong> Force 6/ time 4/ duration 2  2 ul loading with 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-07-6/3dimage_84128/</p>1tgt0006.rec,  Reconstruction (Pixel Size 2.32 nm), 155.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-07-6/3dimage_84128/1tgt0006.rec"
        > <i class="download icon"></i> Download </a></p>

        1tgt0006.mrc,  Tilt Series (Pixel Size 0.58 nm), 761.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-07-6/rawdata/1tgt0006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.29998846
title: 2D thermo-gravity modeling for Southwest Indian Ridge 13–14°E

description: Support data for the manuscript "Does hydrothermal cooling cause extremely thick lithosphere at nearly amagmatic ultraslow-spreading Southwest Indian Ridge 13–14°E?"
DOI: 10.6084/m9.figshare.29998846.v1
title: 2D thermo-gravity modeling for Southwest Indian Ridge 13–14°E

description: Support data for the manuscript "Does hydrothermal cooling cause extremely thick lithosphere at nearly amagmatic ultraslow-spreading Southwest Indian Ridge 13–14°E?"
DOI: 10.22002/ztwsn-02h29
title: stable HAMP mutant

description: UU2612 with stable HAMP
description: <strong>Tilt Series Date:</strong> 2015-03-12</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sandy Parkinson University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB plus gent, Amp, 50 µl 10mM IPTG overnight, penicillin lysis</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-14/rawdata/</p>stableHAMPgrid20007.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-14/rawdata/stableHAMPgrid20007.mrc"
        > <i class="download icon"></i> Download </a></p>

        stableHAMPgrid20007_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-14/3dimage_53216/stableHAMPgrid20007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xrtey-v9979
title: PW1312

description: Pseudomonas aerugionsa strain PW 1312.Acquired from the Transposon mutant collection. (F7 short receptor- PW1312  phoAwp09q2F12)
description: <strong>Tilt Series Date:</strong> 2015-02-23</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
  aba2015-02-23-40_slicer12787.jpg: membrane-bound-cytoplasmic chemoreceptor array (magenta) .  pilus (green) .  flagellar motor (blue)
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-40/rawdata/</p>PseudomonasdeltaF7shortreceptorFeb0004.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-40/rawdata/PseudomonasdeltaF7shortreceptorFeb0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        PseudomonasdeltaF7shortreceptorFeb0004_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-40/3dimage_52261/PseudomonasdeltaF7shortreceptorFeb0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ta2u2k
title: Occurrence Download

description: A dataset containing 8959 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Euonymus hamiltonianus"
 ]
}

 The dataset includes 8959 records from 229 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002176-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/qvzcp-9gk74
title: Vibrio DeltaVC1403

description: Vibrio cholerae strain CO35 (delta VC1403)cytoplasmic chemoreceptor in chemotaxis operon with CheA1(F9 chemotaxis system)
description: <strong>Tilt Series Date:</strong> 2015-01-16</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain generated by Catherine Oikonomou</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown 24 hrs in LB at 30C, diluted 150 µl into 2 ml Ca-HEPES buffer, grown 16 hours at 30C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-01-16-2/rawdata/</p>VibrioCO35tomo0002.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-01-16-2/rawdata/VibrioCO35tomo0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioCO35tomo0002_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-01-16-2/3dimage_50822/VibrioCO35tomo0002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ddw0w-vs548
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-441/rawdata/</p>T4SSWTminiNov18F003_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-441/rawdata/T4SSWTminiNov18F003_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTminiNov18F003_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-441/3dimage_105172/T4SSWTminiNov18F003_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ya5fs-vmm92
title: pED208_27.5k

description: F pilus and t4ss in E. coli
description: <strong>Tilt Series Date:</strong> 2015-01-18</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in LB o/n at 37C</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-18-19/rawdata/</p>pED208_275k0020.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-18-19/rawdata/pED208_275k0020.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED208_275k0020_part121_5.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-18-19/3dimage_50982/pED208_275k0020_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4mrbn-47t66
title: T9_wt

description: This was a wt sample prepared with Paul when he was visiting
description: <strong>Tilt Series Date:</strong> 2016-04-30</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Paul Veith and Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> See pages 84-86</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-04-30-109/rawdata/</p>atlas10099.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-04-30-109/rawdata/atlas10099.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10099.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-04-30-109/3dimage_74723/atlas10099.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7zjt6-r5e89
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-504/rawdata/</p>T4SSWTminiNov21C008_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-504/rawdata/T4SSWTminiNov21C008_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTminiNov21C008_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-504/3dimage_105228/T4SSWTminiNov21C008_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bkbzg-nyr67
title: Vibrio cholerae delta TcpB

description: Raw data files of Vibrio cholerae delta TcpB
description: <strong>Tilt Series Date:</strong> 2015-10-16</p>
<strong>Data Taken By:</strong> Andreas Kjaer</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown ON in LB with ampicillin and IPTG, refreshed in LB shortly prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-114/3dimage_73348/</p>10162015_TcpB_10114_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-114/3dimage_73348/10162015_TcpB_10114_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        10162015_TcpB_10114.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-114/rawdata/10162015_TcpB_10114.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xmde6-cqe75
title: nanochemowt_2ndday

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-11/rawdata/</p>nanochemowt2day_atlas10011.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
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        nanochemowt2day_atlas10011_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
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