DOI: 10.5061/dryad.qbzkh18x1
title: Transcriptome profiling of WT and <em>GbMBL1.1A</em>-overexpressing cotton roots upon <em>V. dahliae</em> infection

description: Cotton, a major economic crop, suffers severe yield losses due to
 Verticillium wilt caused by Verticillium dahliae Kleb., Although the
 lectin receptor-like protein GbMBL1.1A has been identified as a key
 activator of hypersensitive cell death in cotton resistance to this
 pathogen, its downstream defense mechanisms remain unknown. Here, we
 performed comparative transcriptome analyses between the
 GbMBL1.1A-overexpressing (OE) cotton line and its wild-type (WT) recipient
 counterpart (WC) under both normal (non-infected) and V. dahliae-infected
 conditions. A total of 760 differentially expressed genes (DEGs) were
 identified by comparing GbMBL1.1A-OE and WT cotton lines under normal
 condition. Upon pathogen infection across three timepoints, the counts of
 1,679 (1,063 up, 616 down) and 1,648 (633 up, 1,015 down) DEGs were
 identified uniquely from GbMBL1.1A-OE or WT cotton line, respectively,
 relative to their mock controls. Further analysis of these DEGs revealed
 three aspects of changes due to GbMBL1.1A overexpression: 1) Pre-infection
 priming via selective PRR modulation, mitochondrial cell death activation,
 and reactive oxygen species (ROS) antioxidant perturbation under normal
 condition; 2) Significant enhancement of plant defense against pathogen
 through the remodeling of immune receptor activity, alteration of signal
 transduction and regulation, and modulation of secondary metabolic
 processes, with the most significant alteration occurred in lignin and
 phenylpropanoid metabolism. 3) Dynamic redox homeostasis regulation​​
 through metabolite interconversion enzymes during infection process.
 Collectively, these findings ​​strengthen​ the theoretical foundation and
 provide novel candidate targets for developing improved control strategies
 to enhance cotton resistance against V. dahliae.
description: # Transcriptome profiling of WT and GbMBL1.1A-overexpressing cotton roots
 upon V. dahliae infection ## Description of data: File:
 gene.tpm.matrix.annot.xlsx Description: RNA-sequencing expression values
 across all samples in TPM values Number of variables: 24 Number of
 genes/rows: 72761 Raw data expression of 48 samples:
 L1EFC270007--WC_mock_1.R1.raw.fastq.gz
 L1EFC270007--WC_mock_1.R2.raw.fastq.gz
 L1EFC270008--WC_mock_2.R1.raw.fastq.gz
 L1EFC270008--WC_mock_2.R2.raw.fastq.gz
 L1EFC270009--WC_mock_3.R1.raw.fastq.gz
 L1EFC270009--WC_mock_3.R2.raw.fastq.gz
 L1EFC270010--WC_v12h_1.R1.raw.fastq.gz
 L1EFC270010--WC_v12h_1.R2.raw.fastq.gz
 L1EFC270011--WC_v12h_2.R1.raw.fastq.gz
 L1EFC270011--WC_v12h_2.R2.raw.fastq.gz
 L1EFC270012--WC_v12h_3.R1.raw.fastq.gz
 L1EFC270012--WC_v12h_3.R2.raw.fastq.gz
 L1EFC270013--WC_v24h_1.R1.raw.fastq.gz
 L1EFC270013--WC_v24h_1.R2.raw.fastq.gz
 L1EFC270014--WC_v24h_2.R1.raw.fastq.gz
 L1EFC270014--WC_v24h_2.R2.raw.fastq.gz
 L1EFC270015--WC_v24h_3.R1.raw.fastq.gz
 L1EFC270015--WC_v24h_3.R2.raw.fastq.gz
 L1EFC270016--WC_v48h_1.R1.raw.fastq.gz
 L1EFC270016--WC_v48h_1.R2.raw.fastq.gz
 L1EFC270017--WC_v48h_2.R1.raw.fastq.gz
 L1EFC270017--WC_v48h_2.R2.raw.fastq.gz
 L1EFC270018--WC_v48h_3.R1.raw.fastq.gz
 L1EFC270018--WC_v48h_3.R2.raw.fastq.gz
 L1EFC270019--T36_mock_1.R1.raw.fastq.gz
 L1EFC270019--T36_mock_1.R2.raw.fastq.gz
 L1EFC270020--T36_mock_2.R1.raw.fastq.gz
 L1EFC270020--T36_mock_2.R2.raw.fastq.gz
 L1EFC270021--T36_mock_3.R1.raw.fastq.gz
 L1EFC270021--T36_mock_3.R2.raw.fastq.gz
 L1EFC270022--T36_v12h_1.R1.raw.fastq.gz
 L1EFC270022--T36_v12h_1.R2.raw.fastq.gz
 L1EFC270023--T36_v12h_2.R1.raw.fastq.gz
 L1EFC270023--T36_v12h_2.R2.raw.fastq.gz
 L1EFC270024--T36_v12h_3.R1.raw.fastq.gz
 L1EFC270024--T36_v12h_3.R2.raw.fastq.gz
 L1EFC270025--T36_v24h_1.R1.raw.fastq.gz
 L1EFC270025--T36_v24h_1.R2.raw.fastq.gz
 L1EFC270026--T36_v24h_2.R1.raw.fastq.gz
 L1EFC270026--T36_v24h_2.R2.raw.fastq.gz
 L1EFC270027--T36_v24h_3.R1.raw.fastq.gz
 L1EFC270027--T36_v24h_3.R2.raw.fastq.gz
 L1EFC270028--T36_v48h_1.R1.raw.fastq.gz
 L1EFC270028--T36_v48h_1.R2.raw.fastq.gz
 L1EFC270029--T36_v48h_3.R1.raw.fastq.gz
 L1EFC270029--T36_v48h_3.R2.raw.fastq.gz
 L1EFC270030--T36_v48h_4.R1.raw.fastq.gz
 L1EFC270030--T36_v48h_4.R2.raw.fastq.gz