cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.p8cz8w9ss
title: Reconstitution of phase-separated signaling clusters and actin polymerization on supported lipid bilayers

description: Liquid–liquid phase separation driven by weak interactions between
 multivalent molecules contributes to the cellular organization by
 promoting the formation of biomolecular condensates. At membranes, phase
 separation can promote the assembly of transmembrane proteins with their
 cytoplasmic binding partners into micron-sized membrane-associated
 condensates. For example, phase separation promotes clustering of nephrin,
 a transmembrane adhesion molecule, resulting in increased Arp2/3
 complex-dependent actin polymerization. In vitro reconstitution is a
 powerful approach to understanding phase separation in biological systems.
 With a bottom-up approach, we can determine the molecules necessary and
 sufficient for phase separation, map the phase diagram by quantifying
 de-mixing over a range of molecular concentrations, assess the material
 properties of the condensed phase using fluorescence recovery after
 photobleaching (FRAP), and even determine how phase separation impacts
 downstream biochemical activity. Here, we describe a detailed protocol to
 reconstitute nephrin clusters on supported lipid bilayers with purified
 recombinant protein. We also describe how to measure Arp2/3
 complex-dependent actin polymerization on bilayers using fluorescence
 microscopy. These different protocols can be performed independently or
 combined as needed. These general techniques can be applied to
 reconstitute and study phase-separated signaling clusters of many
 different receptors or to generally understand how actin polymerization is
 regulated at membranes.
description: ImageJ or FIJI were used to open the images and conducting some
 of the analyses.
DOI: 10.22008/promice/data/ice_discharge/d/v02/kucaz3
title: GIS_err.csv

description: Greenland error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/v1jo4n
title: GIS.nc

description: Greenland discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/1vsjnj
title: region_coverage.csv

description: Regional coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/9wifrf
title: region_D.csv

description: Regional discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/8w2nsc
title: region_err.csv

description: Regional error. Git hash: 8d24438
DOI: 10.7280/d17d6w
title: Using remote sensing to quantify the additional climate benefits of California forest carbon offset projects

description: Nature-based climate solutions are a vital component of many climate
 mitigation strategies, including California’s, which aims to achieve
 carbon neutrality by 2045. Most carbon offsets in California’s
 cap-and-trade program come from improved forest management projects
 (IFMs). Since 2012, various landowners have set up IFMs following the
 California Air Resources Board’s IFM protocol. As many of these projects
 approach their tenth year, we now have the opportunity to assess their
 effectiveness, identify best practices, and suggest improvements toward
 future protocol revisions. In this study, we used remote sensing-based
 datasets to evaluate the carbon trends and harvest histories of 37 IFMs in
 California. Despite some current limitations and biases, these datasets
 can be used to quantify carbon accumulation and harvest rates in offset
 project lands relative to nearby similar “control” lands before and after
 the projects began. Five lines of evidence suggest that the carbon
 accumulated in offset projects to date has generally not been additional
 to what might have otherwise occurred: (1) most forests in northwestern
 California have been accumulating carbon since at least the mid-1980s and
 continue to accumulate carbon, whether enrolled in offset projects or not;
 (2) harvest rates were high in large timber company project lands before
 IFM initiation, suggesting they are earning carbon credits for forests in
 recovery; (3) projects are often located on lands with higher densities of
 low-timber-value species; (4) carbon accumulation rates have not yet
 increased on lands that enroll as offset projects, relative to their
 pre-enrollment levels; and (5) harvest rates have not decreased on most
 project lands since offset project initiation. These patterns suggest that
 the current protocol should be improved to robustly measure and reward
 additionality. In general, our framework of geospatial analyses offers an
 important and independent means to evaluate the effectiveness of the
 carbon offsets program, especially as these data products continue
 improving and as offsets receive attention as a climate mitigation
 strategy.
description: Data are compiled from a variety of sources in the public domain,
 detailed in the readme.docx. We assemble geospatial datasets for
 remote-sensing-estimated carbon and harvest in Google Earth Engine,
 extract for regions of interest (California offset projects and control
 regions), and generate figures comparing these datasets in Python. See
 "Methods" section of paper for details. 
description: Google Earth Engine Python v3
 Code also available on GitHub: https://github.com/scoffiel/carbon_offsets
DOI: 10.22008/promice/data/ice_discharge/d/v02/ohofkg
title: sector_coverage.csv

description: Sector coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/pb1qch
title: region.nc

description: Regional discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/6yavdg
title: sector_err.csv

description: Sector error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/ebf0iw
title: sector_D.csv

description: Sector discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/4i1xpy
title: sector.nc

description: Sector discharge, coverage, and error. Git hash: 8d24438
DOI: 10.5061/dryad.xd2547dkc
title: Flash sequence duration

description: Unlike light input for forming images, non-image forming retinal pathways
 are optimized to convey information about the total light environment,
 integrating over time and space. It has been demonstrated in a variety of
 species that the retinohypothalamic pathway underlying circadian
 entrainment can effectively integrate the impact of individual flashes
 over time and convey this information as if continuous light exposure had
 occurred. In this study, we examine the extent to which this temporal
 integration can occur. A group of healthy, young (N=20) individuals took
 part in a series of 16-d protocols in which we examined the impact of
 different lengths of light flash exposure sequences on circadian timing.
 We find that a sequence as short as 15 minutes can engender as much change
 in circadian timing as sequences that last for 3.5 hours. Acute
 suppression of melatonin was also observed during short (15-minute)
 exposures, but not in exposures over one hour. Our data are consistent
 with the theory that responses to light flashes are mediated by the
 extrinsic, rod/cone pathway and saturate the response of this pathway
 within 15 minutes. Further excitation leads to no greater change in
 circadian timing and no longer can acutely suppress melatonin, indicating
 that this pathway may be in a refractory state following this brief light
 stimulation. As the light flash sequences can be administered while
 individuals are sleeping, and without waking an individual, these short,
 15-minute exposures may be especially beneficial as a clinical
 intervention for a variety of circadian-based sleep disorders.
description: The phase shift data were obtained through calculation of
 salivary melatonin onset on subsequent nights, with a light intervention
 between the two assessments. The melatonin suppression data were obtained
 as the difference in acute salivary melatonin levels from immediately
 before and at the end of the light intervention. The change in median
 reaction time was obtained from a 10-minute auditory psychomotor vigilance
 task given immediately before and at the end of the light
 intervention. The light intervention was a sequence of
 light flashes. Each light flash last 2 ms, was 2200 lux in illuminance,
 and given with 8 s of darkness between. The duration of the sequence
 varied as indicated, from 15 minutes to 3.5 hours. The light flash
 sequence was centered at 1.5 hours prior to mid-sleep (verified with post
 hoc melatonin phase determination). Melatonin phase was determined on a
 constant routine in dim light.
description: Data are uploaded as a .csv file, which can be opened by a wide
 array of programs.
DOI: 10.22008/promice/data/ice_discharge/d/v02/9cie7q
title: gate_coverage.csv

description: Gate coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/vdahvw
title: gate_D.csv

description: Gate discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/c9ub58
title: gate_err.csv

description: Gate errors. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/qn9ryt
title: gate.nc

description: Gate discharge, coverage, error, and metadata. Git hash: 8d24438
DOI: 10.5061/dryad.m63xsj44h
title: Climate change threatens the future of rainforest ringtail possums by 2050

description: Aim The increasing frequency and intensity of extreme weather escalate the
 pressure of global warming on biodiversity. Globally, synergistic effects
 of multiple components of climate change have driven local extinctions and
 community collapses, raising concern about the irreversible deterioration
 of ecosystems. Here, we disentangle the pressure of different climatic
 components on the population dynamics of a tropical community of
 marsupials in a World Heritage Area. Location The Australian Wet Tropics.
 Method We analyse the potential influence of climate change in different
 dimensions, quantifying the effect of spatial differences in temperature
 exposure and observed increases in temperature and frequency of extreme
 heatwaves. Results We find a strong negative effect of climate change on
 population dynamics, particularly extreme heatwaves, resulting in a rapid
 and severe decline in ringtails’ population size in the last three
 decades. Main conclusions Forecasted increases in temperature and
 heatwaves threaten the collapse of the community by 2050, with ringtail
 possums falling below population viability thresholds within two decades.
DOI: 10.22008/promice/data/ice_discharge/d/v02/rwsagk
title: GIS_D.csv

description: Greenland discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/tihrk5
title: GIS_coverage.csv

description: Greenland coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/ei7z8c
title: GIS_err.csv

description: Greenland error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/kdwnyf
title: region_coverage.csv

description: Regional coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/lcjr4z
title: GIS.nc

description: Greenland discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/bo50s0
title: region_D.csv

description: Regional discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/prtfm1
title: region_err.csv

description: Regional error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/4losey
title: sector_coverage.csv

description: Sector coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/uqfjdz
title: region.nc

description: Regional discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/8x5wez
title: sector_D.csv

description: Sector discharge. Git hash: 8d24438
DOI: 10.5061/dryad.0rxwdbs3b
title: Data from: Maternal effects and the legacy of extreme environmental events for wild mammals

description: Nutrition underpins population dynamics of large herbivores. The
 environmental, physiological, and nutritional state of a mother can have a
 lifetime effect on her offspring. For ungulates, nutrition of the mother
 during gestation can have an important and often underappreciated effect
 on the lifetime phenotype, behavior, and success of her offspring.
 Research in captive settings has shown even with animals that are closely
 related (i.e., have similar genetic makeups), maternal condition can have
 serious lifetime implications for an animal’s offspring; mothers in poor
 condition give birth to sons that exhibit stunted growth compared with
 sons born to mothers in good condition. Yet, identifying the role of
 maternal effects in wild animals can be difficult. It requires information
 on the nutritional legacy of a mother in combination with information on
 current environmental conditions. We evaluated how nutrition of a mother
 potentially affected her male offspring's lifetime trajectory using
 ingesta-free body fat measurements of mule deer (Odocoileus hemionus) in
 the Wyoming Range of western Wyoming, USA. Following a very harsh winter,
 one female gave birth to a male who remained small for the entirety of his
 lifetime, which may be attributed to the nutritional stress his mother
 experienced during that winter. This dataset includes the means and
 standard errors of IFBFat for female deer captured in March from 2013–2021
 and for male deer captured in December from 2018–2021.
description: Nutritional condition of individuals was determined using
 developed protocols for mule deer which include measuring subcutaneous
 rump fat via ultrasonography and body palpation to estimate a body
 condition score (Stephenson et al. 2002; Cook et al. 2007). Using body
 mass, body condition score, and maximum thickness of rump fat via
 ultrasonography, we then estimated ingesta-free body fat (i.e., IFBFat)
 for all individuals (Cook et al. 2010). This dataset
 includes the means and standard errors of IFBFat for female deer captured
 in March from 2013–2021 and for male deer captured in December from
 2018–2021. Cook, R.
 C., Stephenson, T. R., Myers, W. L., Cook, J. G., & Shipley, L. A.
 (2007). Validating Predictive Models of Nutritional Condition for Mule
 Deer. Journal of Wildlife Management, 71(6).
 https://doi.org/10.2193/2006-262 Stephenson, T. R.,
 Bleich, V. C., Pierce, B. M., & Mulcahy, G. P. (2002). Validation
 of mule deer body composition using in vivo and post-mortem indices of
 nutritional condition. Wildlife Society Bulletin, 30(2),
 557–564.
DOI: 10.22008/promice/data/ice_discharge/d/v02/lnhv1o
title: sector_err.csv

description: Sector error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/x42dke
title: sector.nc

description: Sector discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/sfb6va
title: gate_coverage.csv

description: Gate coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/tgmhgo
title: gate_D.csv

description: Gate discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/j1yfld
title: gate_err.csv

description: Gate errors. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/8smfrh
title: gate.nc

description: Gate discharge, coverage, error, and metadata. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/tfwd1v
title: GIS_coverage.csv

description: Greenland coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/ndfxca
title: GIS_D.csv

description: Greenland discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/t5aqri
title: GIS_err.csv

description: Greenland error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/0ygdku
title: GIS.nc

description: Greenland discharge, coverage, and error. Git hash: 8d24438
DOI: 10.5061/dryad.qv9s4mwhh
title: Experimental comparison of fish mortality and injuries at innovative and conventional small hydropower plants - raw data

description: Resolving the controversy about hydropower is only possible based on
 reliable data on its ecological effects, particularly fish welfare.
 Herein, we propose a comprehensive assessment of conventional and
 innovative hydropower using a data set of 52 250 fish. The effects of
 hydropower on fish were most harmful at sites with Kaplan turbines,
 showing £ 83% mortality. Innovative hydropower, often termed
 ‘fish-friendly’, caused £ 64% mortality. Our findings suggested that the
 runner peripheral speed, number of turbine blades, turbulence and gas
 oversaturation at turbine outlets were the most important factors.
 Synthesis and Applications: To reduce the impact of hydropower on fish,
 site-specific characteristics such as head drop, bypass options and
 river-specific species composition need to be more intensively considered
 in optimal turbine technologies and operation modes.
description: These data were generated in a large field experiment to
 investigate the effects of innovative and conventional hydropower turbines
 on fish. The data contain information on external and internal injuries of
 individual fish (Spreadsheet 1) and <br>physical parameters for each
 net emptying interval (Spreadsheet 2).
 <br>External and internal fish injuries caused by
 hydropower turbine passage were classified following the protocols
 published in <br><br>M. Mueller, J. Pander, J. Geist,
 Evaluation of external fish injury caused by hydropower plants based on a
 novel field-based protocol. Fish. Manag. Ecol. 24, 240–255
 (2017).<br>M. Mueller, K. Sternecker, S. Milz, J. Geist, Assessing
 turbine passage effects on internal fish injury and delayed mortality
 using X-ray imaging. PeerJ 8, e9977 (2020).
DOI: 10.22008/promice/data/ice_discharge/d/v02/pphixr
title: region_coverage.csv

description: Regional coverage. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/uhrbq8
title: region_D.csv

description: Regional discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/qym9av
title: region.nc

description: Regional discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/sisbsd
title: region_err.csv

description: Regional error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/cclp6d
title: sector_coverage.csv

description: Sector coverage. Git hash: 8d24438
DOI: 10.5061/dryad.nk98sf7w9
title: Size-selective harvesting affects the immunocompetence of guppies exposed to the parasite Gyrodactylus

description: Harvesting is typically positively size-selective, targeting large
 individuals. This is expected to lead to reduced average body size and
 earlier maturation, i.e., faster life histories. Such changes can also
 affect traits seemingly unrelated to harvesting, including
 immunocompetence. The pace-of-life syndrome predicts that faster life
 histories are correlated with decreased immunocompetence, i.e., a negative
 association between positively size-selective harvesting and
 immunocompetence. However, the energetic trade-off between early growth
 and immunocompetence suggests the opposite pattern. Here, we empirically
 evaluated these predictions using an experimental system consisting of the
 ectoparasite Gyrodactylus turnbulli and lines of guppies Poecilia
 reticulata that had been subjected to either positively, randomly, or
 negatively size-selective harvest. We followed the infection progression
 of individually infected fish for 15 days. We found significant
 differences between the harvested lines: fish from the negative
 size-selection lines had the highest parasite loads. During the early
 phase of the infection, parasite loads were the lowest in the
 positive-harvested lines, whereas the terminal loads were the lowest for
 the randomly harvested lines. These results agree with the predictions
 from the energetic trade-off hypothesis but contradict with the
 pace-of-life syndrome ones. To our knowledge, this is the first
 demonstration of the consequences of size-selective harvesting on
 immunocompetence.
DOI: 10.22008/promice/data/ice_discharge/d/v02/skylbe
title: sector_err.csv

description: Sector error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/yzrhfm
title: sector_D.csv

description: Sector discharge. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/92rfiq
title: sector.nc

description: Sector discharge, coverage, and error. Git hash: 8d24438
DOI: 10.22008/promice/data/ice_discharge/d/v02/kemx9m
title: gate_coverage.csv

description: Gate coverage. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/djpygu
title: gate_D.csv

description: Gate discharge. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/7ksmeq
title: gate_err.csv

description: Gate errors. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/vu0l2w
title: gate.nc

description: Gate discharge, coverage, error, and metadata. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/nrqk7g
title: GIS_coverage.csv

description: Greenland coverage. Git hash: e2d64b1
DOI: 10.5061/dryad.9zw3r22h3
title: Data from: Glacial ice supports a distinct and undocumented polar bear subpopulation persisting in late 21st-century sea-ice conditions

description: Polar bears are susceptible to climate warming because of their dependence
 on sea ice, which is declining rapidly. We present the first evidence for
 a genetically distinct and functionally isolated group of polar bears in
 Southeast Greenland. These bears occupy sea-ice conditions resembling
 those projected for the High Arctic in the late 21st century, with an
 annual ice-free period that is &gt;100 days longer than the estimated
 fasting threshold for the species. Whereas polar bears in most of the
 Arctic depend on annual sea ice to catch seals, Southeast Greenland bears
 have a year-round hunting platform in the form of freshwater glacial
 mélange. This suggests that marine-terminating glaciers, although of
 limited availability, may serve as previously unrecognized climate
 refugia. Conservation of Southeast Greenland polar bears, which meet the
 criteria for recognition as the world’s 20th polar bear subpopulation, is
 necessary to preserve the genetic diversity and evolutionary potential of
 the species.
description: Detailed methods are found in the main text and Supplemental
 material of Laidre et al. (2022). Citation: Kristin L. Laidre, Megan A.
 Supple, Erik W. Born, Eric V. Regehr, Øystein Wiig, Fernando Ugarte, Jon
 Aars, Rune Dietz, Christian Sonne, Peter Hegelund, Carl Isaksen, Geir B.
 Akse, Benjamin Cohen, Harry L. Stern, Twila Moon, Christopher Vollmers,
 Russ Corbett-Detig, David Paetkau, Beth Shapiro. 2022. Data from: Glacial
 ice supports a distinct and undocumented polar bear subpopulation
 persisting in late 21st-century sea-ice conditions, <em>Science
 </em>376 (6599): 1333-1338. https://doi.org/10.1126/science.abk2793
DOI: 10.22008/promice/data/ice_discharge/d/v02/dp3y9u
title: GIS_err.csv

description: Greenland error. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/jgcsdw
title: GIS_D.csv

description: Greenland discharge. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/aik1ti
title: region_coverage.csv

description: Regional coverage. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/dhwkau
title: GIS.nc

description: Greenland discharge, coverage, and error. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/azt7bv
title: region_err.csv

description: Regional error. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/kqjh2s
title: region_D.csv

description: Regional discharge. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/mp0zzo
title: region.nc

description: Regional discharge, coverage, and error. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/ascagm
title: sector_coverage.csv

description: Sector coverage. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/a9zcqi
title: sector_D.csv

description: Sector discharge. Git hash: e2d64b1
DOI: 10.22008/promice/data/ice_discharge/d/v02/zkblm5
title: sector_err.csv

description: Sector error. Git hash: e2d64b1