cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21810309
title: ExtFig6B_RACE_STOML2_R2_LG391.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810309.v1
title: ExtFig6B_RACE_STOML2_R2_LG391.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810312
title: ExtFig6B_RACE_STOML2_R3_LG395_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810312.v1
title: ExtFig6B_RACE_STOML2_R3_LG395_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810315
title: ExtFig6B_RACE_STOML2_R3_LG395.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810315.v1
title: ExtFig6B_RACE_STOML2_R3_LG395.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810249
title: ExtFig6A_RACE_BCAP31_R1_AI20_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810249.v1
title: ExtFig6A_RACE_BCAP31_R1_AI20_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810252
title: ExtFig6A_RACE_BCAP31_R1_AI20.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810252.v1
title: ExtFig6A_RACE_BCAP31_R1_AI20.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810255
title: ExtFig6A_RACE_BCAP31_R2_LG341.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810255.v1
title: ExtFig6A_RACE_BCAP31_R2_LG341.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810258
title: ExtFig6A_RACE_BCAP31_R2_LG341_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810258.v1
title: ExtFig6A_RACE_BCAP31_R2_LG341_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810261
title: ExtFig6A_RACE_SLC7A5_R1_AI21.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810261.v1
title: ExtFig6A_RACE_SLC7A5_R1_AI21.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810264
title: ExtFig6A_RACE_SLC7A5_R2_LG342.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810264.v1
title: ExtFig6A_RACE_SLC7A5_R2_LG342.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810267
title: ExtFig6A_RACE_SLC7A5_R1_AI21.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810267.v1
title: ExtFig6A_RACE_SLC7A5_R1_AI21.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810270
title: ExtFig6A_RACE_SLC7A5_R2_LG342_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810270.v1
title: ExtFig6A_RACE_SLC7A5_R2_LG342_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810273
title: ExtFig6A_RACE_BCAP31_R2_LG341.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810273.v1
title: ExtFig6A_RACE_BCAP31_R2_LG341.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810234.v1
title: ExtFig5E_qPCR_R2_LG302.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810276
title: ExtFig6A_RACE_SLC7A5_R2_LG342.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810276.v1
title: ExtFig6A_RACE_SLC7A5_R2_LG342.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810279
title: ExtFig6A_RACE_SLC7A5_R1_AI21_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810279.v1
title: ExtFig6A_RACE_SLC7A5_R1_AI21_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810204
title: ExtFig4B_RACE_STOML2_YKT6_R1_LG307_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810204.v1
title: ExtFig4B_RACE_STOML2_YKT6_R1_LG307_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810207.v1
title: ExtFig4B_RACE_STOML2_YKT6_R1_LG307.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810234
title: ExtFig5E_qPCR_R2_LG302.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810240
title: ExtFig5E_qPCR_R1_LG301.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810240.v1
title: ExtFig5E_qPCR_R1_LG301.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810207
title: ExtFig4B_RACE_STOML2_YKT6_R1_LG307.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810213.v1
title: ExtFig4B_RACE_STOML2_YKT6_R2_LG308_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810216
title: ExtFig4B_RACE_STOML2_YKT6_R2_LG308.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810216.v1
title: ExtFig4B_RACE_STOML2_YKT6_R2_LG308.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810237
title: ExtFig5E_qPCR_R3_LG303.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810237.v1
title: ExtFig5E_qPCR_R3_LG303.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810210
title: ExtFig4B_RACE_STOML2_YKT6_R1_LG307.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810210.v1
title: ExtFig4B_RACE_STOML2_YKT6_R1_LG307.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810213
title: ExtFig4B_RACE_STOML2_YKT6_R2_LG308_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810219
title: ExtFig4B_RACE_STOML2_YKT6_R2_LG308.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities