DOI: 10.5061/dryad.pc4d6
title: Data from: The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane

description: Msp1 is a conserved AAA ATPase in budding yeast localized to mitochondria
 where it prevents accumulation of mistargeted tail-anchored (TA) proteins,
 including the peroxisomal TA protein Pex15. Msp1 also resides on
 peroxisomes but it remains unknown how native TA proteins on mitochondria
 and peroxisomes evade Msp1 surveillance. We used live-cell quantitative
 cell microscopy tools and drug-inducible gene expression to dissect Msp1
 function. We found that a small fraction of peroxisomal Pex15, exaggerated
 by overexpression, is turned over by Msp1. Kinetic measurements guided by
 theoretical modeling revealed that Pex15 molecules at mitochondria display
 age-independent Msp1 sensitivity. By contrast, Pex15 molecules at
 peroxisomes are rapidly converted from an initial Msp1-sensitive to an
 Msp1-resistant state. Lastly, we show that Pex15 interacts with the
 peroxisomal membrane protein Pex3, which shields Pex15 from Msp1-dependent
 turnover. In sum, our work argues that Msp1 selects its substrates on the
 basis of their solitary membrane existence.
description: Weir_2017_Dryad_READMEREADME with descriptive information and instructions for use of this dataset. Also contains links to GitHub repositories containing Python and R analysis code.Weir_fig_1cMicroscopy source images for Figure 1C. See Weir_2017_Dryad_README.md for additional information.Weir_fig_2c_4dMicroscopy source images for Figures 2C and 4D. See Weir_2017_Dryad_README.md for additional information.Weir_fig_3c_4fMicroscopy source images for Figures 3C and 4F. See Weir_2017_Dryad_README.md for additional information.Weir_fig_3eMicroscopy source images for Figure 3E. See Weir_2017_Dryad_README.md for additional information.Weir_fig_5cMicroscopy source images for Figure 5C. See Weir_2017_Dryad_README.md for additional information.Weir_fig_6cMicroscopy source images for Figure 6C. See Weir_2017_Dryad_README.md for additional information.Weir_fig_6eMicroscopy source images for Figure 6E. See Weir_2017_Dryad_README.md for additional information.Weir_fig_7bMicroscopy source images for Figure 7B. See Weir_2017_Dryad_README.md for additional information.Weir_fig_7cMicroscopy source images for Figure 7C. See Weir_2017_Dryad_README.md for additional information.Weir_fig_7eMicroscopy source images for Figure 7E. See Weir_2017_Dryad_README.md for additional information.Weir_fig_s1a_s1cMicroscopy source images for Figure 1-Figure Supplement 1A and 1C. See Weir_2017_Dryad_README.md for additional information.Weir_fig_s6aMicroscopy source images for Figure 6-Figure Supplement 1A. See Weir_2017_Dryad_README.md for additional information.Weir_fig_s6g_s6hMicroscopy source images for Figure 6-Figure Supplement 1G and 1H. See Weir_2017_Dryad_README.md for additional information.Weir_fig_s6j_pex11Microscopy source images for Figure 6-Figure Supplement 1J (Pex11 portion). See Weir_2017_Dryad_README.md for additional information.Weir_fig_s6j_pex12Microscopy source images for Figure 6-Figure Supplement 1J (Pex12 portion). See Weir_2017_Dryad_README.md for additional information.