DOI: 10.5061/dryad.tht76hf98
title: Data from: The embryonic DPPA3 gene stimulates the expression of pregnancy-related genes in bovine endometrial cells

description: This dataset contains supplementary materials associated with the study
 investigating extracellular vesicle (EV) mRNA transfer from bovine embryos
 to endometrial cells. The dataset includes (1) a table of primers used for
 quantitative PCR (qPCR), specifying primer sequences and target genes; (2)
 an image demonstrating the uptake of GFP mRNA by bovine endometrial
 epithelial cells following EV supplementation, confirming successful mRNA
 translation; and (3) a table of differentially expressed genes from RNA
 sequencing analysis, detailing gene names, log fold changes (logFC),
 p-values, and functional annotations. This dataset provides valuable
 resources for researchers studying EV-mediated communication,
 embryo-maternal interactions, and gene expression changes in response to
 EV-derived mRNA. No ethical or legal restrictions apply to the dataset,
 and it can be freely used for comparative analyses, validation studies, or
 further exploration of transcriptomic changes in reproductive biology.
description: # The embryonic DPPA3 gene stimulates the expression of pregnancy-related
 genes in bovine endometrial cells Dataset DOI:
 [10.5061/dryad.tht76hf98](10.5061/dryad.tht76hf98) ## Description of the
 data and file structure This dataset was generated as part of a study
 investigating the role of extracellular vesicle (EV)-derived mRNA in
 embryo-maternal communication in cattle. The experimental efforts focused
 on identifying full-length mRNA transcripts within EVs secreted by bovine
 embryos and assessing their impact on gene expression in maternal
 endometrial epithelial cells. To achieve this, in vitro fertilized bovine
 embryos were cultured, and EVs were isolated from the culture media. RNA
 sequencing was performed to identify mRNA transcripts within these EVs. A
 key transcript, DPPA3, was selected for further functional analysis.
 Endometrial epithelial cells were supplemented with either embryo-derived
 EVs or in vitro transcribed DPPA3 mRNA to evaluate gene expression changes
 via RNA sequencing and proteomic analysis. This dataset includes: 1. A
 table of primers used for qPCR validation of gene expression changes. 2.
 An image showing GFP mRNA uptake by bovine endometrial epithelial cells
 following supplementation of GFP transfected EVs 3. A table of
 differentially expressed genes identified from RNA sequencing of cells
 transfected with DPPA3 mRNA, including gene names, log fold change
 (logFC), p-values, and functional annotations. This dataset supports
 research on EV-mediated signaling, reproductive biology, and
 transcriptomic changes in embryo-maternal interactions. ### Files and
 variables #### File: Primers\_transcript \_validation.csv
 **Description:** Primers used for transcript validation  #### File:
 DEGsRNASeqDPPA3transfectedVScontrol.csv **Description:** Differential
 expression analysis of RNA-Seq from control vs DPPA3 transfected cells. 
 #### File: EVsTransfectedWithGFP\_inEndoCells.png **Description:** IVF
 derived EVs were transfected with GFP mRNA and subsequently supplemented
 to bovine endometrial epithelial cells. Cells were imaged 24 hours after
 EV supplementation to ensure successful uptake of the mRNA and translation
 into green fluorescent protein.   ## Code/software n/a ## Access
 information Other publicly accessible locations of the data: * RNA
 sequencing data has been deposited in National Center for Biotechnology
 Information (NCBI)’s  Gene Expression Omnibus (GEO) and is accessible
 through GEO Series accession number GSE269049. * All LC-MS/MS RAW files
 and results can be found at the MassIVE database
 ([https://massive.ucsd.edu](https://massive.ucsd.edu)) under the following
 accession MassIVE 000096795.