cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6214331
title: 1035 georeferenced locality records and associated Worldclim bioclimatic variables for North American Bombus huntii

description: Georeferenced locality records of <i>Bombus huntii </i>in North America and associated WorldClim bioclimatic variables (http://www.worldclim.org/): BIO1 = Annual Mean Temperature, BIO2 = Mean Diurnal Range (Mean of monthly (max temp - min temp)), BIO3 = Isothermality (BIO2/BIO7) (* 100), BIO4 = Temperature Seasonality (standard deviation *100), BIO5 = Max Temperature of Warmest Month, BIO6 = Min Temperature of Coldest Month, BIO7 = Temperature Annual Range (BIO5-BIO6), BIO8 = Mean Temperature of Wettest Quarter, BIO9 = Mean Temperature of Driest Quarter, BIO10 = Mean Temperature of Warmest Quarter, BIO11 = Mean Temperature of Coldest Quarter, BIO12 = Annual Precipitation, BIO13 = Precipitation of Wettest Month, BIO14 = Precipitation of Driest Month, BIO15 = Precipitation Seasonality (Coefficient of Variation), BIO16 = Precipitation of Wettest Quarter, BIO17 = Precipitation of Driest Quarter, BIO18 = Precipitation of Warmest Quarter, BIO19 = Precipitation of Coldest Quarter<br>
DOI: 10.6084/m9.figshare.6214331.v1
title: 1035 georeferenced locality records and associated Worldclim bioclimatic variables for North American Bombus huntii

description: p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; line-height: 15.0px; font: 12.0px 'Times New Roman'; color: #3b3b3b; -webkit-text-stroke: #3b3b3b} span.s1 {font-kerning: none; color: #000000; -webkit-text-stroke: 0px #000000} span.s2 {font-kerning: none; background-color: #ffffff} Georeferenced locality records of <i>Bombus huntii </i>in North America and associated WorldClim bioclimatic variables (http://www.worldclim.org/): BIO1 = Annual Mean Temperature, BIO2 = Mean Diurnal Range (Mean of monthly (max temp - min temp)), BIO3 = Isothermality (BIO2/BIO7) (* 100), BIO4 = Temperature Seasonality (standard deviation *100), BIO5 = Max Temperature of Warmest Month, BIO6 = Min Temperature of Coldest Month, BIO7 = Temperature Annual Range (BIO5-BIO6), BIO8 = Mean Temperature of Wettest Quarter, BIO9 = Mean Temperature of Driest Quarter, BIO10 = Mean Temperature of Warmest Quarter, BIO11 = Mean Temperature of Coldest Quarter, BIO12 = Annual Precipitation, BIO13 = Precipitation of Wettest Month, BIO14 = Precipitation of Driest Month, BIO15 = Precipitation Seasonality (Coefficient of Variation), BIO16 = Precipitation of Wettest Quarter, BIO17 = Precipitation of Driest Quarter, BIO18 = Precipitation of Warmest Quarter, BIO19 = Precipitation of Coldest Quarter<br>
DOI: 10.6084/m9.figshare.6214130.v1
title: Mask-modulated lensless imaging with multi-angle illuminations

description: Supplementary materials for the paper: Mask-modulated lensless imaging with multi-angle illuminations.
DOI: 10.6084/m9.figshare.6214127
title: Microsatellite genotypes for 26 Bombus huntii populations

description: Eleven microsatellite genotypes for 350 <i>Bombus huntii </i>(Hymenoptera: Apidae) across 26 populations. This is the final dataset used for analyses by Koch et al. "Quaternary climate instability is correlate with patterns of population genetic variability in Bombus huntii". This filtered dataset accounts for linkage disequilibrium, null alleles, HWE, and sibship relationships. This dataset in in GenAlEx 6.5 format (Peakall R. & Smouse, P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, 28, 2537-2539)
DOI: 10.6084/m9.figshare.6214127.v1
title: Kochetal_BhuntiiMicroSats.xlsx

description: Eleven microsatellite genotypes for 350 <i>Bombus huntii </i>(Hymenoptera: Apidae) across 26 populations. This is the final dataset used for analyses by Koch et al. "Quaternary climate instability is correlate with patterns of population genetic variability in Bombus huntii". This filtered dataset accounts for linkage disequilibrium, null alleles, HWE, and sibship relationships. This dataset in in GenAlEx 6.5 format (Peakall R. & Smouse, P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, 28, 2537-2539)
DOI: 10.6084/m9.figshare.6214127.v2
title: Microsatellite genotypes for 26 North American Bombus huntii populations

description: Eleven microsatellite genotypes for 350 <i>Bombus huntii </i>(Hymenoptera: Apidae) across 26 populations. This is the final dataset used for analyses by Koch et al. "Quaternary climate instability is correlate with patterns of population genetic variability in Bombus huntii". This filtered dataset accounts for linkage disequilibrium, null alleles, HWE, and sibship relationships. This dataset in in GenAlEx 6.5 format (Peakall R. & Smouse, P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, 28, 2537-2539)
DOI: 10.6084/m9.figshare.6214127.v3
title: Microsatellite genotypes for 26 Bombus huntii populations

description: Eleven microsatellite genotypes for 350 <i>Bombus huntii </i>(Hymenoptera: Apidae) across 26 populations. This is the final dataset used for analyses by Koch et al. "Quaternary climate instability is correlate with patterns of population genetic variability in Bombus huntii". This filtered dataset accounts for linkage disequilibrium, null alleles, HWE, and sibship relationships. This dataset in in GenAlEx 6.5 format (Peakall R. & Smouse, P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, 28, 2537-2539)
DOI: 10.6084/m9.figshare.6214043
title: raw data_Qiuxiang Tian.xlsx

description: Raw data of site description and key soil properties along a topographic transect with ridge, middle slope, lower slope and valley positions in a subtropical mountain forest of China.
DOI: 10.6084/m9.figshare.6214043.v1
title: raw data_Qiuxiang Tian.xlsx

description: Raw data of site description and key soil properties along a topographic transect with ridge, middle slope, lower slope and valley positions in a subtropical mountain forest of China.
DOI: 10.6084/m9.figshare.6214037
title: INQDatabase.sav

description: Validation of the interpersonal needs questionnaire of young male adults in Singapore
DOI: 10.17608/k6.auckland.6213965
title: WoRMS_Amphipoda_RawData.xlsx

description: This dataset contains the world list of amphipod crustaceans in the World Register of Marine Species (WoRMS)
DOI: 10.17608/k6.auckland.6213965.v1
title: WoRMS_Amphipoda_RawData.xlsx

description: This dataset contains the world list of amphipod crustaceans in the World Register of Marine Species (WoRMS)
DOI: 10.6084/m9.figshare.6213899
title: Morphological and molecular data support recognition of a new rupicolous species of <i>Pinguicula</i> (<i>Lentibulariaceae</i>) from the Iberian Peninsula

description: A new rupicolous species, <i>Pinguicula saetabensis</i>, belonging to <i>P.</i> sect. <i>Pinguicula</i> is described from calcareous cliffs of central-southern Valencia province, in the eastern Iberian Peninsula. It has previously been confused with <i>P. mundi</i> and <i>P. vallisneriifolia</i>, two close allies endemic to southern Spain which share some morphological traits and a similar habitat. However, some peculiarities allow recognition of those Valencian plants at the specific rank. Data on morphology, ecology, biogeography and conservation are reported for the new species, and its taxonomic affinities are discussed on the basis of phylogenetic analyses of the internal transcribed spacer region of nuclear ribosomal DNA. Furthermore, the presence of one population of <i>P. vallisneriifolia</i> is confirmed in that province, also based on morphological and molecular data.
DOI: 10.6084/m9.figshare.6213899.v1
title: Morphological and molecular data support recognition of a new rupicolous species of <i>Pinguicula</i> (<i>Lentibulariaceae</i>) from the Iberian Peninsula

description: A new rupicolous species, <i>Pinguicula saetabensis</i>, belonging to <i>P.</i> sect. <i>Pinguicula</i> is described from calcareous cliffs of central-southern Valencia province, in the eastern Iberian Peninsula. It has previously been confused with <i>P. mundi</i> and <i>P. vallisneriifolia</i>, two close allies endemic to southern Spain which share some morphological traits and a similar habitat. However, some peculiarities allow recognition of those Valencian plants at the specific rank. Data on morphology, ecology, biogeography and conservation are reported for the new species, and its taxonomic affinities are discussed on the basis of phylogenetic analyses of the internal transcribed spacer region of nuclear ribosomal DNA. Furthermore, the presence of one population of <i>P. vallisneriifolia</i> is confirmed in that province, also based on morphological and molecular data.
DOI: 10.6084/m9.figshare.6213878
title: Flu Vaccine Tweets

description: This project contains two data files in tab-separated values format.<br>annotations.tsv - The first column is the tweet ID, and the second column is the annotation ('yes' or 'no') for whether the tweet indicates that the someone received or intended to receive a flu vaccine. <br>data.tsv - This file contains over 1.2 million tweets containing at least one influenza-related term and one vaccination-related term. The second and third columns are the classifier probabilities that the tweet is positive for vaccine intent (using logistic regression and convolutional neural network, respectively). The remaining columns are the metadata we extracted or inferred (time, location, and gender). Tweets corresponding to the IDs can be downloaded using the Twitter API.
DOI: 10.6084/m9.figshare.6213785
title: Beaulieu C, Lavoie C, Proulx R. 2018. Bookkeeping of insect herbivory trends in herbarium specimens of purple loosestrife (Lythrum salicaria).

description: The potential use of herbarium specimens to detect herbivory trends is enormous but largely untapped. The objective of this study was to reconstruct the long-term herbivory pressure on an invasive plant, purple loosestrife (<i>Lythrum salicaria</i>), by evaluating leaf damages over 1323 specimens from southern Québec (Canada). Historical trends suggest a gradual increase in hole feeding and margin feeding damages from 1883 to around 1940, followed by a period of relative stability. The percentage of specimens with window feeding damages did not begin to increase until the end of the 20th century, from 3% (2 ̶ 6%) in 1990 to 45% (14 ̶ 81%) in 2015. Temporal changes in the frequency of window feeding damages support the hypothesis of an increasing herbivory pressure by recently introduced insects. This study shows that leaf damages made by insects introduced for the biocontrol of purple loosestrife, such as coleopterans of the <i>Neogalerucella</i> genus, can be assessed from voucher specimens. Herbaria are a rich source in information that can be used to answer questions related to plant-insect interactions in the context of biological invasions and biodiversity changes.
DOI: 10.6084/m9.figshare.6213785.v1
title: Beaulieu C, Proulx R, Lavoie C. 2018. Bookkeeping of insect herbivory trends in herbarium specimens of purple loosestrife (Lythrum salicaria).

description: The potential use of herbarium specimens to detect herbivory trends is enormous but largely untapped. The objective of this study was to reconstruct the long-term herbivory pressure on an invasive plant, purple loosestrife (<i>Lythrum salicaria</i>), by evaluating leaf damages over 1323 specimens from southern Québec (Canada). Historical trends suggest a gradual increase in hole feeding and margin feeding damages from 1883 to around 1940, followed by a period of relative stability. The percentage of specimens with window feeding damages did not begin to increase until the end of the 20th century, from 3% (2 ̶ 6%) in 1990 to 45% (14 ̶ 81%) in 2015. Temporal changes in the frequency of window feeding damages support the hypothesis of an increasing herbivory pressure by recently introduced insects. This study shows that leaf damages made by insects introduced for the biocontrol of purple loosestrife, such as coleopterans of the <i>Neogalerucella</i> genus, can be assessed from voucher specimens. Herbaria are a rich source in information that can be used to answer questions related to plant-insect interactions in the context of biological invasions and biodiversity changes.
DOI: 10.6084/m9.figshare.6213785.v3
title: Beaulieu C, Lavoie C, Proulx R. 2018. Bookkeeping of insect herbivory trends in herbarium specimens of purple loosestrife (Lythrum salicaria).

description: The potential use of herbarium specimens to detect herbivory trends is enormous but largely untapped. The objective of this study was to reconstruct the long-term herbivory pressure on an invasive plant, purple loosestrife (<i>Lythrum salicaria</i>), by evaluating leaf damages over 1323 specimens from southern Québec (Canada). Historical trends suggest a gradual increase in hole feeding and margin feeding damages from 1883 to around 1940, followed by a period of relative stability. The percentage of specimens with window feeding damages did not begin to increase until the end of the 20th century, from 3% (2 ̶ 6%) in 1990 to 45% (14 ̶ 81%) in 2015. Temporal changes in the frequency of window feeding damages support the hypothesis of an increasing herbivory pressure by recently introduced insects. This study shows that leaf damages made by insects introduced for the biocontrol of purple loosestrife, such as coleopterans of the <i>Neogalerucella</i> genus, can be assessed from voucher specimens. Herbaria are a rich source in information that can be used to answer questions related to plant-insect interactions in the context of biological invasions and biodiversity changes.
DOI: 10.6084/m9.figshare.6213785.v2
title: Beaulieu C, Lavoie , Proulx R. 2018. Bookkeeping of insect herbivory trends in herbarium specimens of purple loosestrife (Lythrum salicaria).

description: The potential use of herbarium specimens to detect herbivory trends is enormous but largely untapped. The objective of this study was to reconstruct the long-term herbivory pressure on an invasive plant, purple loosestrife (<i>Lythrum salicaria</i>), by evaluating leaf damages over 1323 specimens from southern Québec (Canada). Historical trends suggest a gradual increase in hole feeding and margin feeding damages from 1883 to around 1940, followed by a period of relative stability. The percentage of specimens with window feeding damages did not begin to increase until the end of the 20th century, from 3% (2 ̶ 6%) in 1990 to 45% (14 ̶ 81%) in 2015. Temporal changes in the frequency of window feeding damages support the hypothesis of an increasing herbivory pressure by recently introduced insects. This study shows that leaf damages made by insects introduced for the biocontrol of purple loosestrife, such as coleopterans of the <i>Neogalerucella</i> genus, can be assessed from voucher specimens. Herbaria are a rich source in information that can be used to answer questions related to plant-insect interactions in the context of biological invasions and biodiversity changes.
DOI: 10.6084/m9.figshare.6213698.v1
title: Tissue residue depletion of moxidectin in lambs (<i>Ovis aries</i>) following subcutaneous administration

description: To date, a tissue depletion study of moxidectin (MOX) in lambs is not available. Thus, considering that lamb meat is of great commercial interest in the world, the aim of the present study was to determine the residue levels of MOX in lamb target-tissues (muscle, liver, kidney, and fat) and subsequently calculate the MOX withdrawal period. For this purpose, the target-tissues were analysed by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Method validation was performed based on Commission Decision 2002/657/EC and VICH GL49. To quantify the analyte, matrix-matched analytical curves were constructed with spiked blank tissues. The limits of detection and quantitation were 1.5 ng g<sup>−1</sup> and 5 ng g<sup>−1</sup>, respectively for all matrices. The linearity, decision limit, detection capability accuracy, and inter- and intra-day precision of the method are reported. The lambs were treated with a single subcutaneous (s.c.) dose of 0.2 mg MOX kg<sup>−1</sup> body weight (bw), and were slaughtered in accordance with accepted animal care protocols. Samples of target-tissues were collected on 2, 4, 7, 14, 28, and 42 days after MOX administration. During the whole study, the highest drug residue level occurred in the fat. For the other target-tissues (muscle, liver, and kidney), MOX concentrations were below the maximum residue limit (MRL). Considering the MRL value of 500 µg kg<sup>−1</sup> for MOX residues in sheep fat, our results in lambs allowed the estimation of a MOX withdrawal period of 31 days. This indicates that the withdrawal period established for MOX in adult sheep (28 days) do not apply for lambs.
DOI: 10.6084/m9.figshare.6213623
title: High-throughput behavioral assay to investigate seizure sensitivity in zebrafish implicates <i>ZFHX3</i> in epilepsy

description: Epilepsy, which affects ∼1% of the population, is caused by abnormal synchronous neural activity in the central nervous system (CNS). While there is a significant genetic contribution to epilepsy, the underlying causes for the majority of genetic cases remain unknown. The NIH Undiagnosed Diseases Project (UDP) utilized exome sequencing to identify genetic variants in patients affected by various conditions with undefined etiology, including epilepsy. Confirming the functional relevance of the candidate genes identified by exome sequencing in a timely manner is crucial to translating exome data into clinically useful information. To this end, we developed a high throughput version of a seizure-sensitivity assay in zebrafish (<i>Danio rerio</i>) to rapidly evaluate candidate genes found by exome sequencing. We developed open access software, Studying Epilepsy In Zebrafish using R (SEIZR), to efficiently analyze the data. SEIZR was validated by disrupting function of a known epilepsy gene, <i>prickle 1</i>. Next, using SEIZR, we analyzed a candidate gene from the UDP screen (<i>Zinc Finger Homeobox 3</i>, <i>ZFHX3</i>), and showed that reduced <i>ZFHX3</i> function in zebrafish results in a significant hyperactive response to the convulsant drug pentylenetetrazol (PTZ). We find that <i>ZFHX3</i> shows strong expression in the CNS during neurogenesis including in the pallium, thalamus, tegmentum, reticular formation, and medulla oblongata – all regions which have roles in motor control and coordination. Our findings in the zebrafish confirm human <i>ZFHX3</i> is a strong candidate for further neurological studies. We offer SEIZR to other researchers as a tool to rapidly and efficiently analyze large behavioral data sets.
DOI: 10.6084/m9.figshare.6213623.v1
title: High-throughput behavioral assay to investigate seizure sensitivity in zebrafish implicates <i>ZFHX3</i> in epilepsy

description: Epilepsy, which affects ∼1% of the population, is caused by abnormal synchronous neural activity in the central nervous system (CNS). While there is a significant genetic contribution to epilepsy, the underlying causes for the majority of genetic cases remain unknown. The NIH Undiagnosed Diseases Project (UDP) utilized exome sequencing to identify genetic variants in patients affected by various conditions with undefined etiology, including epilepsy. Confirming the functional relevance of the candidate genes identified by exome sequencing in a timely manner is crucial to translating exome data into clinically useful information. To this end, we developed a high throughput version of a seizure-sensitivity assay in zebrafish (<i>Danio rerio</i>) to rapidly evaluate candidate genes found by exome sequencing. We developed open access software, Studying Epilepsy In Zebrafish using R (SEIZR), to efficiently analyze the data. SEIZR was validated by disrupting function of a known epilepsy gene, <i>prickle 1</i>. Next, using SEIZR, we analyzed a candidate gene from the UDP screen (<i>Zinc Finger Homeobox 3</i>, <i>ZFHX3</i>), and showed that reduced <i>ZFHX3</i> function in zebrafish results in a significant hyperactive response to the convulsant drug pentylenetetrazol (PTZ). We find that <i>ZFHX3</i> shows strong expression in the CNS during neurogenesis including in the pallium, thalamus, tegmentum, reticular formation, and medulla oblongata – all regions which have roles in motor control and coordination. Our findings in the zebrafish confirm human <i>ZFHX3</i> is a strong candidate for further neurological studies. We offer SEIZR to other researchers as a tool to rapidly and efficiently analyze large behavioral data sets.
DOI: 10.6084/m9.figshare.6213614
title: An RNAi-mediated screen identifies novel targets for next-generation antiepileptic drugs based on increased expression of the homeostatic regulator pumilio

description: Despite availability of a diverse range of anti-epileptic drugs (AEDs), only about two-thirds of epilepsy patients respond well to drug treatment. Thus, novel targets are required to catalyse the design of next-generation AEDs. Manipulation of neuron firing-rate homoeostasis, through enhancing Pumilio (Pum) activity, has been shown to be potently anticonvulsant in <i>Drosophila</i>. In this study, we performed a genome-wide RNAi screen in S2R + cells, using a luciferase-based dPum activity reporter and identified 1166 genes involved in dPum regulation. Of these genes, we focused on 699 genes that, on knock-down, potentiate dPum activity/expression. Of this subgroup, 101 genes are activity-dependent based on comparison with genes previously identified as activity-dependent by RNA-sequencing. Functional cluster analysis shows these genes are enriched in pathways involved in DNA damage, regulation of cell cycle and proteasomal protein catabolism. To test for anticonvulsant activity, we utilised an RNA-interference approach <i>in vivo</i>. RNAi-mediated knockdown showed that 57/101 genes (61%) are sufficient to significantly reduce seizure duration in the characterized seizure mutant, <i>para<sup>bss</sup></i>. We further show that chemical inhibitors of protein products of some of the genes targeted are similarly anticonvulsant. Finally, to establish whether the anticonvulsant activity of identified compounds results from increased <i>dpum</i> transcription, we performed a luciferase-based assay to monitor <i>dpum</i> promoter activity. Third instar larvae exposed to sodium fluoride, gemcitabine, metformin, bestatin, WP1066 or valproic acid all showed increased <i>dpum</i> promoter activity. Thus, this study validates Pum as a favourable target for AED design and, moreover, identifies a number of lead compounds capable of increasing the expression of this homeostatic regulator.