cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25380/iastate.13175180
title: Salt-induced coil-to-globular transition and interfacial assembly in poly(N-isopropylacrylamide)-capped gold nanoparticles

description: This dataset contains the transmission electron microscopy imaging acquired in the liquid phase, video files acquired form the electron microscope screen, accompanied by the image analysis data, and with the x-ray reflectivity and electron density profiles along with the data analysis obtained form the air/liquid interface, of the aqueous solution containing poly(N-isopropylacrylamide)-capped gold nanoparticles in response to addition of NaCl solution to the system.
DOI: 10.25380/iastate.13175180.v1
title: Salt-induced coil-to-globular transition and interfacial assembly in poly(N-isopropylacrylamide)-capped gold nanoparticles

description: This dataset contains the transmission electron microscopy imaging acquired in the liquid phase, video files acquired form the electron microscope screen, accompanied by the image analysis data, and with the x-ray reflectivity and electron density profiles along with the data analysis obtained form the air/liquid interface, of the aqueous solution containing poly(N-isopropylacrylamide)-capped gold nanoparticles in response to addition of NaCl solution to the system.
DOI: 10.6084/m9.figshare.22606904.v1
title: Additional file 3 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 3. The correlation matrix of three biological replicates of the EIF5A2-OE group and the control group.
DOI: 10.6084/m9.figshare.22606910.v1
title: Additional file 5 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 5. The upregulated DEGs of EIF5A2-OE group vs Control group.
DOI: 10.6084/m9.figshare.22606922.v1
title: Additional file 7 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 7. Correlation of UPR-related genes and EIF5A2.
DOI: 10.6084/m9.figshare.22606931
title: Additional file 10 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 10. Correlation analysis between differentially expressed TFs and the validated UPR-related genes.
DOI: 10.6084/m9.figshare.22606913.v1
title: Additional file 6 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 6. Representative GO biological processes of down-regulated DEGs.
DOI: 10.6084/m9.figshare.22606922
title: Additional file 7 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 7. Correlation of UPR-related genes and EIF5A2.
DOI: 10.6084/m9.figshare.22606925.v1
title: Additional file 8 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 8. Differentially expressed TFs in EIF5A2-OE group.
DOI: 10.6084/m9.figshare.22606901
title: Additional file 2 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 2. RNA-seq reads of SH-SY5Y cells from control group and EIF5A2-OE group.
DOI: 10.6084/m9.figshare.22606910
title: Additional file 5 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 5. The upregulated DEGs of EIF5A2-OE group vs Control group.
DOI: 10.6084/m9.figshare.22606925
title: Additional file 8 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 8. Differentially expressed TFs in EIF5A2-OE group.
DOI: 10.6084/m9.figshare.22606934
title: Additional file 11 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 11. Correlation analysis between DEGs and EIF5A2 in human glial cell tissue database.
DOI: 10.6084/m9.figshare.22606928
title: Additional file 9 of EIF5A2 specifically regulates the transcription of aging-related genes in human neuroblastoma cells

description: Additional file 9. Analysis of the potential target genes regulated by BHLHE40, RHOXF1, and TBX20.
DOI: 10.6084/m9.figshare.22607105
title: Additional file 4 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 4. KEGG enrichment analysis of DEGs.
DOI: 10.6084/m9.figshare.22607102
title: Additional file 3 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 3.  GO enrichment analysis of DEGs.
DOI: 10.6084/m9.figshare.22607102.v1
title: Additional file 3 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 3.  GO enrichment analysis of DEGs.
DOI: 10.6084/m9.figshare.22607108.v1
title: Additional file 5 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 5. DEGs involved in carbohydrate metabolism.
DOI: 10.6084/m9.figshare.22607120
title: Additional file 8 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 8. DEGs involved in the development ovule of Arabidopsis in Paeonia ludlowii.
DOI: 10.6084/m9.figshare.22607108
title: Additional file 5 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 5. DEGs involved in carbohydrate metabolism.
DOI: 10.6084/m9.figshare.22607096
title: Additional file 1 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 1. qRT-PCR primers information.
DOI: 10.6084/m9.figshare.22607096.v1
title: Additional file 1 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 1. qRT-PCR primers information.
DOI: 10.6084/m9.figshare.22607105.v1
title: Additional file 4 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 4. KEGG enrichment analysis of DEGs.
DOI: 10.6084/m9.figshare.22607111
title: Additional file 6 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 6. DEGs involved in phytohormones signaling of Paeonia ludlowii.
DOI: 10.6084/m9.figshare.22607114
title: Additional file 7 of Transcriptome sequencing and gene expression analysis revealed early ovule abortion of Paeonia ludlowii

description: Additional file 7. DEGs involved in PCD of Paeonia ludlowii.
DOI: 10.6084/m9.figshare.8848361
title: Cooking effects on bioactive compounds and sensory acceptability in pumpkin (Cucurbita moschata cv. Leite)

description: ABSTRACT The present study aimed to compare the changes in the bioactive compounds, color parameters and to determine consumer opinions through sensory analysis of the pumpkin using different cooking methods (boiling, steaming, microwaving and sous vide). The cooking methods reduced the ascorbic acid content (49.73-50.42%) and polyphenols (49.68-64.94%). Microwaving increased the carotenoid content by 24.58%. The brightness (L*), red color (a*) and yellow color (b*) were reduced and there was also the loss of vivid color (chroma). The color of samples was affected after cooking process. All cooking methods were well accepted by the tasters, representing different market niches and many consumers expressed the intention of purchasing these products. The main component analysis also indicated the acceptance of different treatments for three distinct groups of assessors, one formed by boiling and steam treatments and two others formed by sous vide and microwave alone. Pumpkins contain high levels of functional components, and microwaving is the preferred cooking method to promote their retention, which can be performed with enough guarantees that the product will always be purchased by consumers.
DOI: 10.6084/m9.figshare.8848361.v1
title: Cooking effects on bioactive compounds and sensory acceptability in pumpkin (Cucurbita moschata cv. Leite)

description: ABSTRACT The present study aimed to compare the changes in the bioactive compounds, color parameters and to determine consumer opinions through sensory analysis of the pumpkin using different cooking methods (boiling, steaming, microwaving and sous vide). The cooking methods reduced the ascorbic acid content (49.73-50.42%) and polyphenols (49.68-64.94%). Microwaving increased the carotenoid content by 24.58%. The brightness (L*), red color (a*) and yellow color (b*) were reduced and there was also the loss of vivid color (chroma). The color of samples was affected after cooking process. All cooking methods were well accepted by the tasters, representing different market niches and many consumers expressed the intention of purchasing these products. The main component analysis also indicated the acceptance of different treatments for three distinct groups of assessors, one formed by boiling and steam treatments and two others formed by sous vide and microwave alone. Pumpkins contain high levels of functional components, and microwaving is the preferred cooking method to promote their retention, which can be performed with enough guarantees that the product will always be purchased by consumers.
DOI: 10.6084/m9.figshare.8848148
title: Action of two endocrine disrupters on the sexual differenciation of Nile tilapia

description: ABSTRACT Endocrine disruptors are exogenous substances that can impair one or more functions of the endocrine system and cause adverse effects to the health of an intact organism. The main goal of this study was to evaluate the effect of the endocrine disruptors 17β-estradiol (E2) and 17α-ethinyl estradiol (EE2) on the sexual differentiation of Nile tilapia during spawning. Six treatments and one control with three replications, totalizing 21 experimental units, were assessed in a completely randomized design. Hormones were dosed at concentrations of 250, 500, and 1,000 ng L−1 (treatments E2-250, E2-500, E2-1,000, EE2-250, EE2-500, and EE2-1,000). Exposure to hormones occurred during the first 28 days of life. After this period, water was completely renewed and individuals kept in their respective experimental units for another 60 days, totaling 88 days. At the end of this period, animals were euthanized by ice desensitization for gonad extraction, slide preparation, and observation under an optical microscope. At the end of the experiment, the results indicated that the type of hormone and hormone concentration differentially affected the parameters of zootechnical performance. A significant difference (p&lt;0.05) was observed in the condition factor between control and treatments. Intersex individuals were observed in treatments of both hormones, except for the control treatment. In addition, malformations were observed in individuals submitted to treatments, mainly in the head region. Thus, the presence of the chemical compounds 17β-estradiol and 17α-ethinylestradiol severely affected the development and sexual differentiation of Nile tilapia.
DOI: 10.6084/m9.figshare.8848148.v1
title: Action of two endocrine disrupters on the sexual differenciation of Nile tilapia

description: ABSTRACT Endocrine disruptors are exogenous substances that can impair one or more functions of the endocrine system and cause adverse effects to the health of an intact organism. The main goal of this study was to evaluate the effect of the endocrine disruptors 17β-estradiol (E2) and 17α-ethinyl estradiol (EE2) on the sexual differentiation of Nile tilapia during spawning. Six treatments and one control with three replications, totalizing 21 experimental units, were assessed in a completely randomized design. Hormones were dosed at concentrations of 250, 500, and 1,000 ng L−1 (treatments E2-250, E2-500, E2-1,000, EE2-250, EE2-500, and EE2-1,000). Exposure to hormones occurred during the first 28 days of life. After this period, water was completely renewed and individuals kept in their respective experimental units for another 60 days, totaling 88 days. At the end of this period, animals were euthanized by ice desensitization for gonad extraction, slide preparation, and observation under an optical microscope. At the end of the experiment, the results indicated that the type of hormone and hormone concentration differentially affected the parameters of zootechnical performance. A significant difference (p&lt;0.05) was observed in the condition factor between control and treatments. Intersex individuals were observed in treatments of both hormones, except for the control treatment. In addition, malformations were observed in individuals submitted to treatments, mainly in the head region. Thus, the presence of the chemical compounds 17β-estradiol and 17α-ethinylestradiol severely affected the development and sexual differentiation of Nile tilapia.
DOI: 10.6084/m9.figshare.8848508
title: Germination and biochemical changes in West Indian gherkin seeds under water stress at different temperatures

description: ABSTRACT Information on the effects of climate conditions on the survival of species grown in semi-arid regions is essential and at the same time scarce, especially regarding rustic species such as West Indian gherkin (Cucumis anguria L.). Thus, this study aimed to evaluate germination, growth and biochemical alterations in C. anguria seeds under water stress and different temperature regimes. The experimental design was completely randomized, in 5 x 5 factorial scheme, composed of five osmotic potentials (0.0; -0.1; -0.2; -0.3 and -0.4 MPa) and four temperatures (20; 25; 30 °C and alternating temperatures of 20-30 ºC), with each treatment composed of four replicates of 50 seeds. The seeds were sown on paper towel (Germitest®) and germinated in Biochemical Oxygen Demand (B.O.D.) germinators, under 8-h photoperiod. Germination percentage, germination speed index, mean time of germination, seedling length and contents of chlorophylls, carotenoids, proline, free amino acids and free sugars were determined along the experiment. Decrease in osmotic potential reduced germination, growth and contents of chloroplast pigments of C. anguria seedlings, but was more drastic at levels lower than -0.2 MPa. Low temperatures intensify the effects of water stress on the germination of C. anguria seeds. The synthesis of protecting osmolytes increased in C. anguria seedlings but these components did not promote efficient osmotic adjustment in their initial development stage.
DOI: 10.6084/m9.figshare.8848508.v1
title: Germination and biochemical changes in West Indian gherkin seeds under water stress at different temperatures

description: ABSTRACT Information on the effects of climate conditions on the survival of species grown in semi-arid regions is essential and at the same time scarce, especially regarding rustic species such as West Indian gherkin (Cucumis anguria L.). Thus, this study aimed to evaluate germination, growth and biochemical alterations in C. anguria seeds under water stress and different temperature regimes. The experimental design was completely randomized, in 5 x 5 factorial scheme, composed of five osmotic potentials (0.0; -0.1; -0.2; -0.3 and -0.4 MPa) and four temperatures (20; 25; 30 °C and alternating temperatures of 20-30 ºC), with each treatment composed of four replicates of 50 seeds. The seeds were sown on paper towel (Germitest®) and germinated in Biochemical Oxygen Demand (B.O.D.) germinators, under 8-h photoperiod. Germination percentage, germination speed index, mean time of germination, seedling length and contents of chlorophylls, carotenoids, proline, free amino acids and free sugars were determined along the experiment. Decrease in osmotic potential reduced germination, growth and contents of chloroplast pigments of C. anguria seedlings, but was more drastic at levels lower than -0.2 MPa. Low temperatures intensify the effects of water stress on the germination of C. anguria seeds. The synthesis of protecting osmolytes increased in C. anguria seedlings but these components did not promote efficient osmotic adjustment in their initial development stage.
DOI: 10.6084/m9.figshare.19690819
title: Supplementary Material for: Examining the combination of cefixime and amoxicillin/clavulanate against extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates

description: Introduction: Community-acquired urinary tract infections (UTIs) caused by extended spectrum beta-lactamase (ESBL)-producing Escherichia coli have limited oral therapeutic options and pose significant clinical challenges. The goal of this study was to evaluate the in vitro synergy between CFM and AMC against ESBL E. coli with aims to identify an oral treatment option for UTIs. Methods: Minimum inhibitory concentrations (MICs) of CFM in the presence of AMC were determined for 46 clinical isolates by placing a CFM Etest on a plate with AMC impregnated in the agar. Isolates with CFM MIC ≤1 µg/ml in the presence of AMC were considered susceptible to the CFM and AMC combination. Five isolates were then selected for further testing using time-kill analysis in the presence of CFM, AMC, and CFM with AMC. Time-kill curves were plotted to determine synergy over 24 hours. Results: AMC improved the activity of CFM against ESBL E. coli isolates by 128-fold in the Etest analysis with 85% of tested isolates being susceptible to the combination. Four-fold or greater reduction in CFM MIC was exhibited in 44 of 46 (96%) isolates when in the presence of AMC. Synergy and bactericidal activity between CFM and AMC were exhibited in each of the five isolates tested by time-kill analysis. Discussion/Conclusion: This study found that AMC improves the activity of CFM against ESBL E. coli and that this antibiotic combination has potential as an oral therapeutic option to treat ESBL E. coli UTIs.
DOI: 10.6084/m9.figshare.19690819.v1
title: Supplementary Material for: Examining the combination of cefixime and amoxicillin/clavulanate against extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates

description: Introduction: Community-acquired urinary tract infections (UTIs) caused by extended spectrum beta-lactamase (ESBL)-producing Escherichia coli have limited oral therapeutic options and pose significant clinical challenges. The goal of this study was to evaluate the in vitro synergy between CFM and AMC against ESBL E. coli with aims to identify an oral treatment option for UTIs. Methods: Minimum inhibitory concentrations (MICs) of CFM in the presence of AMC were determined for 46 clinical isolates by placing a CFM Etest on a plate with AMC impregnated in the agar. Isolates with CFM MIC ≤1 µg/ml in the presence of AMC were considered susceptible to the CFM and AMC combination. Five isolates were then selected for further testing using time-kill analysis in the presence of CFM, AMC, and CFM with AMC. Time-kill curves were plotted to determine synergy over 24 hours. Results: AMC improved the activity of CFM against ESBL E. coli isolates by 128-fold in the Etest analysis with 85% of tested isolates being susceptible to the combination. Four-fold or greater reduction in CFM MIC was exhibited in 44 of 46 (96%) isolates when in the presence of AMC. Synergy and bactericidal activity between CFM and AMC were exhibited in each of the five isolates tested by time-kill analysis. Discussion/Conclusion: This study found that AMC improves the activity of CFM against ESBL E. coli and that this antibiotic combination has potential as an oral therapeutic option to treat ESBL E. coli UTIs.
DOI: 10.6084/m9.figshare.8848187
title: Physiological maturity of Physalis angulata L. seeds

description: ABSTRACT Determination of seed physiological maturity allows predicting the most appropriate time for harvest, aiming to obtain seeds with high physiological potential. Physalis angulata (Solanaceae) has pharmacological and agro-industrial potential, but there is little information on its reproductive phenology. Thus, this study aimed to evaluate the physiological maturity of P. angulata seeds according to fruit age. Fruits were harvested at 7, 14, 21, 28 and 35 days after anthesis (DAA) and the following evaluations were conducted: fruit weight and diameter, seed moisture content, 100-seed dry weight, 1000-seed weight, electrical conductivity, germination, germination first count, emergence, and seedling dry weight and length. The experimental design was completely randomized. Fruit weight and diameter increased until 35 DAA and seed dry weight increased until 21 DAA. Highest seed physiological potential occurred at 35 DAA, with highest germination percentage (97%). The results allow concluding that P. angulata seeds become physiologically viable from 21 DAA, with highest physiological potential at 35 DAA. However, harvest should be carried out around 28 DAA, before fruits fall.
DOI: 10.6084/m9.figshare.8848250
title: The use of X-ray to evaluate Brachiaria brizantha seeds quality during seed processing

description: ABSTRACT The X-ray test permits the visualization of the internal structures of seeds in a non-destructive way making thus possible the diagnosis of causes of seed low germination and purity. The objective of this project was to evaluate the quality of Brachiaria brizantha seeds by means of the X-ray test and the effect of the processing stage. The seeds were sampled before processing and after going through the air and screen machine, and the first and the second gravity tables. Seeds were evaluated as to water content, purity, weight of a thousand seeds, germination, and the X-ray test. The treatment replications were distributed according to a complete random design with four replications. The X-ray test permit to verify the completely full seeds as well as deteriorated, hollow, mechanically damaged seeds and empty spikelets. The processing procedure caused no damage to the seeds. The analysis by means of X-ray is a quality control procedure resulting in information that can be used in the adjustments of processing machines.
DOI: 10.6084/m9.figshare.8848250.v1
title: The use of X-ray to evaluate Brachiaria brizantha seeds quality during seed processing

description: ABSTRACT The X-ray test permits the visualization of the internal structures of seeds in a non-destructive way making thus possible the diagnosis of causes of seed low germination and purity. The objective of this project was to evaluate the quality of Brachiaria brizantha seeds by means of the X-ray test and the effect of the processing stage. The seeds were sampled before processing and after going through the air and screen machine, and the first and the second gravity tables. Seeds were evaluated as to water content, purity, weight of a thousand seeds, germination, and the X-ray test. The treatment replications were distributed according to a complete random design with four replications. The X-ray test permit to verify the completely full seeds as well as deteriorated, hollow, mechanically damaged seeds and empty spikelets. The processing procedure caused no damage to the seeds. The analysis by means of X-ray is a quality control procedure resulting in information that can be used in the adjustments of processing machines.