DOI: 10.22002/xh28v-x0d35
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-43/3dimage_69085/</p>12jan20_C110_3_pNL_INFalpha_AT1BT10019_SIRT.rec,  Reconstruction (Pixel Size 2.0 nm), 2.9 GB  
        
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        12jan20_C110_3_pNL_INFalpha_AT1BT10019_full.rec,  Reconstruction (Pixel Size 2.0 nm), 660.6 MB  
        
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        12jan20_C110_3_pNL_INFalpha_AT1BT10019.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        12jan20_C110_3_pNL_INFalpha_AT1BT10019_SIRT_2a.tiff,  file associated to 3D Image #69085, 373.0 kB  
        
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        12jan20_C110_3_pNL_INFalpha_AT1BT10019_SIRT_1c.tiff,  file associated to 3D Image #69085, 638.7 kB  
        
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        12jan20_C110_3_pNL_INFalpha_AT1BT10019_SIRT_1.tiff,  file associated to 3D Image #69085, 613.0 kB  
        
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