cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20012860
title: Urea and salt as supplementary diet for crossbreed milk cows

description: This study aimed to evaluate the use of supplementary feed with different percentages of urea and mineral salt for crossbred milk cows. Ten animals were used, mean body weight 480 kg ± 9,7, distributed in a 5 x 5 double Latin square, with treatments of supplements with different urea and mineral salt percentages, namely, 10:10; 10:20; 20:10 and 20:20, completed to 100% with corn meal. Data underwent statistical analysis by SAS at 0.05 significance level. The use of supplements increased (p &lt; 0.05) the total dry matter (DM) intake of diet. The increase in the level of urea in the supplement decreased DM intake of supplement. The use of supplements increased (p &lt; 0.05) DM digestibility. The treatments with presence of supplement showed higher (p &lt; 0.05) milk production. Supplemented diets may be used to correct nutritional deficiencies of sugar cane, with higher intake, digestibility and milk production. Treatments with 20% urea in the composition of the supplement caused a lower DM consumption with the same performance in the animals. The above-mentioned treatments had the best efficiency.
DOI: 10.6084/m9.figshare.20012860.v1
title: Urea and salt as supplementary diet for crossbreed milk cows

description: This study aimed to evaluate the use of supplementary feed with different percentages of urea and mineral salt for crossbred milk cows. Ten animals were used, mean body weight 480 kg ± 9,7, distributed in a 5 x 5 double Latin square, with treatments of supplements with different urea and mineral salt percentages, namely, 10:10; 10:20; 20:10 and 20:20, completed to 100% with corn meal. Data underwent statistical analysis by SAS at 0.05 significance level. The use of supplements increased (p &lt; 0.05) the total dry matter (DM) intake of diet. The increase in the level of urea in the supplement decreased DM intake of supplement. The use of supplements increased (p &lt; 0.05) DM digestibility. The treatments with presence of supplement showed higher (p &lt; 0.05) milk production. Supplemented diets may be used to correct nutritional deficiencies of sugar cane, with higher intake, digestibility and milk production. Treatments with 20% urea in the composition of the supplement caused a lower DM consumption with the same performance in the animals. The above-mentioned treatments had the best efficiency.
DOI: 10.6084/m9.figshare.20012859
title: Propolis extract and sodium monensin on ruminal fermentation and hematological parameters in sheep

description: This study evaluated the effects of different concentrations of 30% propolis ethanol extract (PEE) and sodium monensin on the intake of dry matter, nutrient digestibility and ruminal fermentation and hematological parameters in sheep. Six adult castrated male sheep fistulated in the rumen were assigned to a 6 x 6 Latin square design. The addition of PEE or sodium monensin to the diet did not change the digestibility coefficients of dry matter (79.4%), crude protein (77.0%), neutral detergent fiber (76.1%), acid detergent fiber (69.7%), hemicellulose (80.9%), and organic matter (79.4%). Sheep fed diets with inclusion of sodium monensin showed 11.3% reduction in dry matter intake (1.76 kg day-1) compared to those which received PEE (2.00 kg day-1). The inclusion of additives to the diet influenced ruminal pH: higher values were observed in sheep fed sodium monensin (6.1). Mean levels of ammonia nitrogen (7.3 mg dL-1) were similar between treatments. Biochemical serum parameters of glucose (59.4 mg dL-1), urea (8.9 mg dL-1) total protein (6.8 g dL-1) and albumin (2.5 g dL-1) were within the reference range for the ovine species. Sodium monensin was more efficient to maintain ruminal pH at higher levels and to reduce the dry matter intake. However, the addition of PEE did not affect the nutrient digestibility in sheep fed 50:50 forage: concentrate ratio.
DOI: 10.6084/m9.figshare.20012859.v1
title: Propolis extract and sodium monensin on ruminal fermentation and hematological parameters in sheep

description: This study evaluated the effects of different concentrations of 30% propolis ethanol extract (PEE) and sodium monensin on the intake of dry matter, nutrient digestibility and ruminal fermentation and hematological parameters in sheep. Six adult castrated male sheep fistulated in the rumen were assigned to a 6 x 6 Latin square design. The addition of PEE or sodium monensin to the diet did not change the digestibility coefficients of dry matter (79.4%), crude protein (77.0%), neutral detergent fiber (76.1%), acid detergent fiber (69.7%), hemicellulose (80.9%), and organic matter (79.4%). Sheep fed diets with inclusion of sodium monensin showed 11.3% reduction in dry matter intake (1.76 kg day-1) compared to those which received PEE (2.00 kg day-1). The inclusion of additives to the diet influenced ruminal pH: higher values were observed in sheep fed sodium monensin (6.1). Mean levels of ammonia nitrogen (7.3 mg dL-1) were similar between treatments. Biochemical serum parameters of glucose (59.4 mg dL-1), urea (8.9 mg dL-1) total protein (6.8 g dL-1) and albumin (2.5 g dL-1) were within the reference range for the ovine species. Sodium monensin was more efficient to maintain ruminal pH at higher levels and to reduce the dry matter intake. However, the addition of PEE did not affect the nutrient digestibility in sheep fed 50:50 forage: concentrate ratio.
DOI: 10.6084/m9.figshare.20012862
title: Maize kernel size and texture: production parameters, quality of eggs of the laying hens and electricity intake

description: The influence of maize corn size and texture on the performance parameters of laying hens and power consumption required for grinding maize corn were evaluated. The experiment was carried out on 384 Isa Brown hens, 36 weeks old, penned in a conventional aviary with 562.5 cm2 bird-1 stocking rate. The treatments were distributed in a completely randomized 2 x 3 factorial design (maize textures: flint and dent; and milling degree: fine, medium and coarse) with eight replicates of eight birds per plot. Data were evaluated with SISVAR and means were compared by Tukey's test at 5% probability. Difference was reported for the variable texture and flint increased the variables feed intake and egg weight. Significant difference in the characteristics of egg quality occurred only for the colorof the yolk. Larger corn sizes consumed less electricity during grinding. The maize flint cultivar had a lower 31.7% power consumption when compared to that of the dent cultivar.
DOI: 10.6084/m9.figshare.20012862.v1
title: Maize kernel size and texture: production parameters, quality of eggs of the laying hens and electricity intake

description: The influence of maize corn size and texture on the performance parameters of laying hens and power consumption required for grinding maize corn were evaluated. The experiment was carried out on 384 Isa Brown hens, 36 weeks old, penned in a conventional aviary with 562.5 cm2 bird-1 stocking rate. The treatments were distributed in a completely randomized 2 x 3 factorial design (maize textures: flint and dent; and milling degree: fine, medium and coarse) with eight replicates of eight birds per plot. Data were evaluated with SISVAR and means were compared by Tukey's test at 5% probability. Difference was reported for the variable texture and flint increased the variables feed intake and egg weight. Significant difference in the characteristics of egg quality occurred only for the colorof the yolk. Larger corn sizes consumed less electricity during grinding. The maize flint cultivar had a lower 31.7% power consumption when compared to that of the dent cultivar.
DOI: 10.6084/m9.figshare.20012863
title: Milk production and economic assessment of cassava bagasse in the feed of dairy cows

description: The addition of 0; 5; 10 and 15% cassava bagasse, based on the dry matter of the total diet of crossbred Holstein v. Zebu cows, was evaluated on milk production and composition and on the impacts of diet costs. The animals, weighing an average of 478.5 kg, were in the middle third lactation period. Diet with 15% cassava bagasse provided a 13.2% increase in production when compared to control. Feed conversion had a quadratic effect with minimum point at 4.2% of cassava bagasse inclusion. Crude protein, the only milk component that changed, increased linearly with the inclusion of cassava bagasse levels. Treatment with 15% cassava bagasse caused a more effective operational cost (42.8% higher when compared to control) and the highest leveling point for milk production and price. The lowest leveling points were treatments with 5 and 10% inclusion of cassava bagasse, which had the best economic results. Concentrates caused cost increase, particularly when roughage : concentrate ratio decreased due to higher cassava bagasse inclusion levels.
DOI: 10.6084/m9.figshare.20012863.v1
title: Milk production and economic assessment of cassava bagasse in the feed of dairy cows

description: The addition of 0; 5; 10 and 15% cassava bagasse, based on the dry matter of the total diet of crossbred Holstein v. Zebu cows, was evaluated on milk production and composition and on the impacts of diet costs. The animals, weighing an average of 478.5 kg, were in the middle third lactation period. Diet with 15% cassava bagasse provided a 13.2% increase in production when compared to control. Feed conversion had a quadratic effect with minimum point at 4.2% of cassava bagasse inclusion. Crude protein, the only milk component that changed, increased linearly with the inclusion of cassava bagasse levels. Treatment with 15% cassava bagasse caused a more effective operational cost (42.8% higher when compared to control) and the highest leveling point for milk production and price. The lowest leveling points were treatments with 5 and 10% inclusion of cassava bagasse, which had the best economic results. Concentrates caused cost increase, particularly when roughage : concentrate ratio decreased due to higher cassava bagasse inclusion levels.
DOI: 10.5281/zenodo.6350922
title: MD simulation of POPC bilayer with OPLS3e force field, 500 mM CaCl2 part 1

description: MD simulation of POPC bilayer with OPLS3e force field, 500 mM CaCl<sub>2</sub> part 1 Dataset contains trajectories (_trj) for the first 500ns of the 1000ns trajectory, topology (-out.cms) and input files (.cfg, .msj, .cms). For the ease of the upload, trajectory files (_trj) are divided to 100ns pieces and tarred (named desmond_md_cacl500_x-xns.tar.gz) Dataset also contains Gromacs converted files (.xtc, .gro and .top). Converted trajectories are as 100 ns pieces for analysis since simulation did not equilibrate during 1000 ns. System: POPC bilayer in water Number of lipids: 200 (100/leaflet) Number of waters: 8880 Salt: CaCl<sub>2</sub> Concentration: 500 mM Number of cations: 80 Simulation time: 1000 ns (in this dataset 0-500ns) Simulation engine: Desmond 2019-4 Temperature: 300 K Related dataset: MD simulation of POPC bilayer with OPLS3e force field, 500 mM CaCl<sub>2</sub> part 2
DOI: 10.6084/m9.figshare.20012865.v1
title: Feeding Nile tilapia with artificial diets and dried bioflocs biomass

description: ABSTRACT. The effects of supplying dried bioflocs biomass to Nile tilapia juveniles were investigated in the present work. Fish were stocked in experimental rearing tanks for eight weeks. Selected variables of water quality, growth performance and effluent quality were evaluated. Fish were given different combinations of artificial diets and biofloc biomass, in wet (in natura) or dry form. As the supply of artificial diet decreased, the allowance of biofloc biomass increased in the same proportion. Biofloc biomass was produced in two 500-L tanks that received daily applications of dry molasses to adjust the C: N ratio of the water to 15: 1. The concentration of nitrite in water was only reduced in the tanks receiving wet bioflocs. The fish growth performance was poorer in the tanks that received dried biofloc biomass than in the tanks with wet biofloc biomass. Given the lack of positive results, there is no justification to dry the biofloc biomass aiming at delivering it as a dry biomass to reared fish.
DOI: 10.6084/m9.figshare.20012865
title: Feeding Nile tilapia with artificial diets and dried bioflocs biomass

description: ABSTRACT. The effects of supplying dried bioflocs biomass to Nile tilapia juveniles were investigated in the present work. Fish were stocked in experimental rearing tanks for eight weeks. Selected variables of water quality, growth performance and effluent quality were evaluated. Fish were given different combinations of artificial diets and biofloc biomass, in wet (in natura) or dry form. As the supply of artificial diet decreased, the allowance of biofloc biomass increased in the same proportion. Biofloc biomass was produced in two 500-L tanks that received daily applications of dry molasses to adjust the C: N ratio of the water to 15: 1. The concentration of nitrite in water was only reduced in the tanks receiving wet bioflocs. The fish growth performance was poorer in the tanks that received dried biofloc biomass than in the tanks with wet biofloc biomass. Given the lack of positive results, there is no justification to dry the biofloc biomass aiming at delivering it as a dry biomass to reared fish.
DOI: 10.6084/m9.figshare.20012869
title: Histological characterization of oocyte developmental stages of suruvi Steindachneridion scriptum kept in captivity

description: ABSTRACT. Stages of oocyte development were described for suruvi females (Steindachneridion scriptum) kept in captivity. Oocytes of 21 mature or maturing females and ovaries of 12 immature females were fixed in Karnovsky solution for 4 and 12 hours, respectively. After, they were processed for routine histology, embedded in paraffin and stained with hematoxylin-eosin. Slides were examined under a light microscope and the oocytes were measured using the LAS EZ software, using the average between the largest and the smallest diameter of each oocyte for representation. There were six types of oocytes: Chromatin nucleolus: found in immature females, with average diameter of 9.5 ± 4.46 μm; Perinucleolar: present in all developmental stages with average diameter of 67.6 ± 15.61 μm; Cortical alveolar: measuring 175.6 ± 56.58 μm; Vitellogenic: average diameter of 797.65 ± 136.10 μm; Mature: with average diameter of 1184.98 ± 171.13 μm and Atretic, which were found in mature or maturing females of suruvi.
DOI: 10.6084/m9.figshare.20012869.v1
title: Histological characterization of oocyte developmental stages of suruvi Steindachneridion scriptum kept in captivity

description: ABSTRACT. Stages of oocyte development were described for suruvi females (Steindachneridion scriptum) kept in captivity. Oocytes of 21 mature or maturing females and ovaries of 12 immature females were fixed in Karnovsky solution for 4 and 12 hours, respectively. After, they were processed for routine histology, embedded in paraffin and stained with hematoxylin-eosin. Slides were examined under a light microscope and the oocytes were measured using the LAS EZ software, using the average between the largest and the smallest diameter of each oocyte for representation. There were six types of oocytes: Chromatin nucleolus: found in immature females, with average diameter of 9.5 ± 4.46 μm; Perinucleolar: present in all developmental stages with average diameter of 67.6 ± 15.61 μm; Cortical alveolar: measuring 175.6 ± 56.58 μm; Vitellogenic: average diameter of 797.65 ± 136.10 μm; Mature: with average diameter of 1184.98 ± 171.13 μm and Atretic, which were found in mature or maturing females of suruvi.
DOI: 10.6084/m9.figshare.20012871
title: Additives in ensiling palisade grass managed under grazing intensities

description: ABSTRACT. Conservation of summer forage excess represents a management strategy to meet animals' needs for dry matter in the shortage period, but has been poorly studied. Silage can be used for this purpose. This study analyzed the production of palisade grass silage from pasture subjected to different grazing intensities with and without additive, determining losses by gases and effluents and chemical composition of silage. The experiment was a 4 x 3 factorial completely randomized design, with four replications. The factors were: 1st - herbage allowance of 5% (5 kg dry matter 100 kg-1 of animal weight day-1), 10, 15 and 20%. The pasture was managed under rotational stocking with 35-day grazing cycles (7 days of occupation and 28 days of rest) and 2nd - additives: a) control; b) citrus pulp pellets; c) biological inoculant for grass silage. The forage of palisade grass harvested from pastures subjected to low-intensity grazing showed quantitative and qualitative characteristics for ensiling. However, high humidity and low fermentable carbohydrate require the use of additive, favor the fermentation process and increase the nutritional quality of silage.
DOI: 10.6084/m9.figshare.20012871.v1
title: Additives in ensiling palisade grass managed under grazing intensities

description: ABSTRACT. Conservation of summer forage excess represents a management strategy to meet animals' needs for dry matter in the shortage period, but has been poorly studied. Silage can be used for this purpose. This study analyzed the production of palisade grass silage from pasture subjected to different grazing intensities with and without additive, determining losses by gases and effluents and chemical composition of silage. The experiment was a 4 x 3 factorial completely randomized design, with four replications. The factors were: 1st - herbage allowance of 5% (5 kg dry matter 100 kg-1 of animal weight day-1), 10, 15 and 20%. The pasture was managed under rotational stocking with 35-day grazing cycles (7 days of occupation and 28 days of rest) and 2nd - additives: a) control; b) citrus pulp pellets; c) biological inoculant for grass silage. The forage of palisade grass harvested from pastures subjected to low-intensity grazing showed quantitative and qualitative characteristics for ensiling. However, high humidity and low fermentable carbohydrate require the use of additive, favor the fermentation process and increase the nutritional quality of silage.
DOI: 10.6084/m9.figshare.20012874
title: Internal quality of laying hen eggs fed on protease at different storage and stocking conditions

description: ABSTRACT. Effects on diets with reduction of crude protein and methionine + cystine (RCP) and protease supplementation (PRO) for laying hens at different ages and their influence on the internal quality of eggs at different conditions (STO) and storage times (TM) are evaluated. Four hundred and twenty eggs from commercial Hy-Line W36 laying hens collected at 32, 44 and 58 weeks of age were used. Experimental design was completely randomized in a factorial scheme 3 x 2 x 2 x 2: RCP (control, enhancement of one and two times the enzyme protease), PRO (with and without protease), STO (room temperature and refrigerated) and TM (14 and 28 days), with seven replications of one egg per experimental unit. The factorial scheme 3 x 2 x 2 was used at 58 weeks of age, because TM could not be evaluated. The percentages of egg weight loss, Haugh unit, albumen percentage, percentage and yolk index were evaluated after each storage time. RCP and PRO had no effect on internal egg quality whereas eggs under refrigeration preserved their internal quality regardless of the different storage periods.
DOI: 10.6084/m9.figshare.20012874.v1
title: Internal quality of laying hen eggs fed on protease at different storage and stocking conditions

description: ABSTRACT. Effects on diets with reduction of crude protein and methionine + cystine (RCP) and protease supplementation (PRO) for laying hens at different ages and their influence on the internal quality of eggs at different conditions (STO) and storage times (TM) are evaluated. Four hundred and twenty eggs from commercial Hy-Line W36 laying hens collected at 32, 44 and 58 weeks of age were used. Experimental design was completely randomized in a factorial scheme 3 x 2 x 2 x 2: RCP (control, enhancement of one and two times the enzyme protease), PRO (with and without protease), STO (room temperature and refrigerated) and TM (14 and 28 days), with seven replications of one egg per experimental unit. The factorial scheme 3 x 2 x 2 was used at 58 weeks of age, because TM could not be evaluated. The percentages of egg weight loss, Haugh unit, albumen percentage, percentage and yolk index were evaluated after each storage time. RCP and PRO had no effect on internal egg quality whereas eggs under refrigeration preserved their internal quality regardless of the different storage periods.
DOI: 10.6084/m9.figshare.20012877.v1
title: Additional file 1 of Low-cost sample preservation methods for high-throughput processing of rumen microbiomes

description: Additional file1. Supplementary tables and figures. Table S1. Dry matter (DM) of the GRC method samples using the freeze dryer. Table S2. DNA yield and DNA quality for rumen samples preserved using the GRC method, TNx2, GHx2 and EtOH. Table S3. DNA yield and quality of sheep rumen samples using four sample preservation methods (expanded). Table S4 Differences in relative abundance of genera using four different preservation methods. Fig. S1 PCR plate layout for the four different libraries.
DOI: 10.6084/m9.figshare.20012880.v1
title: Additional file 2 of Low-cost sample preservation methods for high-throughput processing of rumen microbiomes

description: Additional file2. Supplementary data. Data S1. Sample and library information of 1208 samples preserved using the four preservation methods and 32 positive controls sequenced using RE-RRS. Data S2. Number of reads generated from RE-RRS for 1208 samples preserved using the four preservation methods and 72 control sample (32 positive and 40 negative). Data S3. Count matrix of reads assigned to genus level taxonomy using the RB approach for 1208 samples preserved using the four preservation methods and 72 control sample (32 positive and 40 negative).
DOI: 10.6084/m9.figshare.20012880
title: Additional file 2 of Low-cost sample preservation methods for high-throughput processing of rumen microbiomes

description: Additional file2. Supplementary data. Data S1. Sample and library information of 1208 samples preserved using the four preservation methods and 32 positive controls sequenced using RE-RRS. Data S2. Number of reads generated from RE-RRS for 1208 samples preserved using the four preservation methods and 72 control sample (32 positive and 40 negative). Data S3. Count matrix of reads assigned to genus level taxonomy using the RB approach for 1208 samples preserved using the four preservation methods and 72 control sample (32 positive and 40 negative).
DOI: 10.6084/m9.figshare.20012886
title: Additional file 4 of Low-cost sample preservation methods for high-throughput processing of rumen microbiomes

description: Additional file4. Supplementary Code. R code used to produce the analysis.
DOI: 10.6084/m9.figshare.20012877
title: Additional file 1 of Low-cost sample preservation methods for high-throughput processing of rumen microbiomes

description: Additional file1. Supplementary tables and figures. Table S1. Dry matter (DM) of the GRC method samples using the freeze dryer. Table S2. DNA yield and DNA quality for rumen samples preserved using the GRC method, TNx2, GHx2 and EtOH. Table S3. DNA yield and quality of sheep rumen samples using four sample preservation methods (expanded). Table S4 Differences in relative abundance of genera using four different preservation methods. Fig. S1 PCR plate layout for the four different libraries.
DOI: 10.6084/m9.figshare.20012886.v1
title: Additional file 4 of Low-cost sample preservation methods for high-throughput processing of rumen microbiomes

description: Additional file4. Supplementary Code. R code used to produce the analysis.