cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6318584.v1
title: PREOPERATIVE ELECTROCARDIOGRAPHIC STUDY OF DOGS AT THE VETERINARY HOSPITAL OF PONTIFÍCIA UNIVERSIDADE CATÓLICA DO PARANÁ

description: Abstract Electrocardiogram is considered a substantial instrument in the clinical evaluation of patients with heart disease. This tool can be used to determine the origin and frequency of the impulse as well as the cardiac conduction disorders. The aim of this study was to evaluate the frequency and type of electrocardiographic abnormalities in the immediate preoperative period of 60 dogs seen in the surgery department of the Veterinary Hospital of Pontifícia Universidade Católica do Paraná - PUCPR, campus Toledo, between September 2015 and June 2017, and to analyze its correlation with the different breeds, sex, age, and weight of each patient. Alterations in the preoperative electrocardiogram have been shown to be frequent and their occurrence is independent of age, breed, size, and sex of the animal. The most frequent findings in the present study were the following: respiratory sinus arrhythmia (30%), atrial fibrillation (16,7%), sinus tachycardia (10%), increase in R wave amplitude (10%), and ST segment depression (10%). Therefore, electrocardiography proved to be an auxiliary diagnostic method of great relevance in the dogs' preoperative period, guaranteeing greater anesthesia safety and lower risks of cardiovascular complications.
DOI: 10.6084/m9.figshare.6318596
title: DIGITAL CONTRAST VENOGRAPHY IN VIVO OF THE FORELIMB AND HINDLIMB IN HEALTHY SHEEP AND GOAT

description: Abstract The venographic examination allows diagnosing and treating foot diseases. It can be the basis of studies about the administration of medication in the hoof region. The aim of the study was to describe the venographic pattern in the distal region of the forelimb and hindlimbs of healthy sheep and goats, as well as to compare vascularization between them. Ten clinically healthy sheep and goats, five male and five female of different breeds, aged 2 to 4 years and weighing 45 to 63 kg were used. The forelimbs and hindlimbs were subjected to contrast venography of the distal region. The venograms were taken in a digital X -ray machine and analyzed in the EcoView® software. The veins of the metacarpus and metatarsus; and the proximal, middle and distal phalanges of the forelimbs and hindlimbs of sheep and goats were identified 25-30 seconds after contrast injection. The technique error observed during the venography was the contrast perivascular extravasation. The venograms showed the vascularization in the distal region of forelimbs and hindlimbs and the communication of vessels between them. The better radiographic view to evaluate the vessels was the lateral projection. The normal venous pattern of the distal region of forelimbs and hindlimbs of sheep and goats could be observed by venography.
DOI: 10.6084/m9.figshare.6318596.v1
title: DIGITAL CONTRAST VENOGRAPHY IN VIVO OF THE FORELIMB AND HINDLIMB IN HEALTHY SHEEP AND GOAT

description: Abstract The venographic examination allows diagnosing and treating foot diseases. It can be the basis of studies about the administration of medication in the hoof region. The aim of the study was to describe the venographic pattern in the distal region of the forelimb and hindlimbs of healthy sheep and goats, as well as to compare vascularization between them. Ten clinically healthy sheep and goats, five male and five female of different breeds, aged 2 to 4 years and weighing 45 to 63 kg were used. The forelimbs and hindlimbs were subjected to contrast venography of the distal region. The venograms were taken in a digital X -ray machine and analyzed in the EcoView® software. The veins of the metacarpus and metatarsus; and the proximal, middle and distal phalanges of the forelimbs and hindlimbs of sheep and goats were identified 25-30 seconds after contrast injection. The technique error observed during the venography was the contrast perivascular extravasation. The venograms showed the vascularization in the distal region of forelimbs and hindlimbs and the communication of vessels between them. The better radiographic view to evaluate the vessels was the lateral projection. The normal venous pattern of the distal region of forelimbs and hindlimbs of sheep and goats could be observed by venography.
DOI: 10.6084/m9.figshare.7510622
title: SEROEPIDEMIOLOGICAL INQUIRY OF ENZOOTIC BOVINE LEUKOSIS IN CATTLE HERDS IN THE STATE OF SERGIPE

description: Abstract The state of Sergipe, located in the Northeast of Brazil, is the only state in the region with absence of studies on the seroprevalence of Enzootic Bovine Leukosis. Therefore, there is a need of research to verify the current status of the disease. The aim of this study was to conduct a seroepidemiological inquiry of enzootic bovine leukosis in cattle herds in the state of Sergipe, which was divided into three mesoregions: East Sergipe, Agreste Sergipe (dry and wild area of Sergipe) and Sertão Sergipe (arid, badlands of Sergipe). In each mesoregion, we selected the five counties with the largest cattle population, totaling 780 samples collected from males and females at all ages, from 52 farms of the visited counties. All serum samples were submitted to the test of agar gel immunodiffusion (AGID) and positive results were obtained for anti-virus antibodies of bovine leukosis (anti-VLB) in 11.92% (93/780) of the animals. Among the farms, 59.61% (31/52) presented seropositive animals, and from the 15 counties visited, only two of them did not show seropositive animals. According to the results, the presence of the etiologic agent in cattle in Sergipe was confirmed, reinforcing the need to institute effective measures of control and prevention against Enzootic bovine, in order to control the disease in the state and better understand its epidemiology.
DOI: 10.6084/m9.figshare.7510622.v1
title: SEROEPIDEMIOLOGICAL INQUIRY OF ENZOOTIC BOVINE LEUKOSIS IN CATTLE HERDS IN THE STATE OF SERGIPE

description: Abstract The state of Sergipe, located in the Northeast of Brazil, is the only state in the region with absence of studies on the seroprevalence of Enzootic Bovine Leukosis. Therefore, there is a need of research to verify the current status of the disease. The aim of this study was to conduct a seroepidemiological inquiry of enzootic bovine leukosis in cattle herds in the state of Sergipe, which was divided into three mesoregions: East Sergipe, Agreste Sergipe (dry and wild area of Sergipe) and Sertão Sergipe (arid, badlands of Sergipe). In each mesoregion, we selected the five counties with the largest cattle population, totaling 780 samples collected from males and females at all ages, from 52 farms of the visited counties. All serum samples were submitted to the test of agar gel immunodiffusion (AGID) and positive results were obtained for anti-virus antibodies of bovine leukosis (anti-VLB) in 11.92% (93/780) of the animals. Among the farms, 59.61% (31/52) presented seropositive animals, and from the 15 counties visited, only two of them did not show seropositive animals. According to the results, the presence of the etiologic agent in cattle in Sergipe was confirmed, reinforcing the need to institute effective measures of control and prevention against Enzootic bovine, in order to control the disease in the state and better understand its epidemiology.
DOI: 10.6084/m9.figshare.7515887
title: ANTIHELMINTHIC EFFICACY OF IVERMECTIN IN HORSES: COPROPARASITOLOGICAL AND HEMATOLOGICAL EXAMS

description: Abstract The aim of the present study was to evaluate the efficacy of ivermectin at 2% in horses, through coproparasitological and hematological exams. The experiment was performed at a farm in the city of Uberlândia, Minas Gerais State. Twenty-four horses were used, ranging from 3 to 8 years of age, divided into 2 groups according to EPG values. EPG counts and coprocultures were performed on days D0, D+7 and D+14. The animals were treated with the ivermectin 2%-based paste formulation at a dose of 200 mcg/kg, orally. Blood samples were collected on days D0, D+7 and D+14 for evaluation of haematological parameters. The presence of eggs that are characteristic of the Strongylidae family and the identification of larvae that are characteristic of cyathostomins demonstrated the parasitism of the animals by small strongyles. At D+7 and D+14 there was a significant reduction of EPG in the treated group with an efficacy of 99.5 and 99.7%, respectively. A tendency of the erythrocyte framework to remain close to the lower limits was observed, besides a slight leukocytosis, with neutrophils close to the highest values. It can be inferred that ivermectin is effective against cyastostomines, and no significant haematological changes were observed.
DOI: 10.6084/m9.figshare.7515887.v1
title: ANTIHELMINTHIC EFFICACY OF IVERMECTIN IN HORSES: COPROPARASITOLOGICAL AND HEMATOLOGICAL EXAMS

description: Abstract The aim of the present study was to evaluate the efficacy of ivermectin at 2% in horses, through coproparasitological and hematological exams. The experiment was performed at a farm in the city of Uberlândia, Minas Gerais State. Twenty-four horses were used, ranging from 3 to 8 years of age, divided into 2 groups according to EPG values. EPG counts and coprocultures were performed on days D0, D+7 and D+14. The animals were treated with the ivermectin 2%-based paste formulation at a dose of 200 mcg/kg, orally. Blood samples were collected on days D0, D+7 and D+14 for evaluation of haematological parameters. The presence of eggs that are characteristic of the Strongylidae family and the identification of larvae that are characteristic of cyathostomins demonstrated the parasitism of the animals by small strongyles. At D+7 and D+14 there was a significant reduction of EPG in the treated group with an efficacy of 99.5 and 99.7%, respectively. A tendency of the erythrocyte framework to remain close to the lower limits was observed, besides a slight leukocytosis, with neutrophils close to the highest values. It can be inferred that ivermectin is effective against cyastostomines, and no significant haematological changes were observed.
DOI: 10.6084/m9.figshare.7509197
title: FETAL BOVINE PRIMARY CULTURE CELLS SUBMITTED TO DIFFERENT NEGATIVE PRESSURES BEFORE STRAW FREEZING

description: Abstract Cell cryopreservation is an important tool in the preservation of endangered species. Fetal bovine primary culture-obtained cells were subjected to negative pressure (NP) 200, 500 or 800 mbar, just before (NP0h) or 3 hours before (NP3h) freezing into fine (0.25 mL) straws, using 10% DMSO as cryoprotectant. Fresh and frozen fibroblasts non submitted to NP were used as controls. We evaluated cell viability after freezing, cell proliferation curve, and population doubling time (PDT) every 24 hours during 8 days. Data were submitted to Tukey or Chi square test (P≤ 0.05). The average survival of control group (89.8%) and NP500-0h (88.1%) was higher than other groups; the time of PDT was similar in the fresh (27.5 ± 0.35 h), frozen control (30.1 ± 2.3 h) and NP500-0h groups (32.4 ± 1.6 h). The lowest time was observed in the NP800-0h (21.9 h) group. Freezing of bovine fibroblasts into 0.25 mL straws with 10% DMSO enables high survival rates after thawing. The NP modifies the growth curve of cryopreserved cells and the intensities of 200 or 500 mbar, applied just before freezing the cells, allow proliferation curves similar to those obtained with fresh cells.
DOI: 10.6084/m9.figshare.7509197.v1
title: FETAL BOVINE PRIMARY CULTURE CELLS SUBMITTED TO DIFFERENT NEGATIVE PRESSURES BEFORE STRAW FREEZING

description: Abstract Cell cryopreservation is an important tool in the preservation of endangered species. Fetal bovine primary culture-obtained cells were subjected to negative pressure (NP) 200, 500 or 800 mbar, just before (NP0h) or 3 hours before (NP3h) freezing into fine (0.25 mL) straws, using 10% DMSO as cryoprotectant. Fresh and frozen fibroblasts non submitted to NP were used as controls. We evaluated cell viability after freezing, cell proliferation curve, and population doubling time (PDT) every 24 hours during 8 days. Data were submitted to Tukey or Chi square test (P≤ 0.05). The average survival of control group (89.8%) and NP500-0h (88.1%) was higher than other groups; the time of PDT was similar in the fresh (27.5 ± 0.35 h), frozen control (30.1 ± 2.3 h) and NP500-0h groups (32.4 ± 1.6 h). The lowest time was observed in the NP800-0h (21.9 h) group. Freezing of bovine fibroblasts into 0.25 mL straws with 10% DMSO enables high survival rates after thawing. The NP modifies the growth curve of cryopreserved cells and the intensities of 200 or 500 mbar, applied just before freezing the cells, allow proliferation curves similar to those obtained with fresh cells.
DOI: 10.6084/m9.figshare.7510616
title: OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES

description: Abstract The objective of this study was to identify the occurrence of genes expressing staphylococcal enterotoxin (sea, seb, sec, and seg) and the toxin gene 1 of toxic shock syndrome (tst) in Staphylococcus aureus coming from cases of bovine mastitis in the state of Pernambuco, Brazil. We analyzed 93 isolates and we observed the presence of toxigenic gene in 20 (21.6%) samples, of which 11 (55.0%) were positive for tst gene, seven (35.0%) for the sec gene, and two (10.0%) for the seg gene. Of the 20 isolates amplified in PCR for the presence of the sec gene, seg and tst, 16 (80.0%) were positive for only one gene and four (20.0%) were positive for two genes (sec and tst). Of the 17 properties from which the samples originated, seven (41.2%) had positive samples for at least one of the genes sec, seg, and tst. This is the first record of the occurrence of the genes encoding the enterotoxin SEC and TST-1 in milk samples from cows with mastitis in the state of Pernambuco, Brazil.
DOI: 10.6084/m9.figshare.7510616.v1
title: OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES

description: Abstract The objective of this study was to identify the occurrence of genes expressing staphylococcal enterotoxin (sea, seb, sec, and seg) and the toxin gene 1 of toxic shock syndrome (tst) in Staphylococcus aureus coming from cases of bovine mastitis in the state of Pernambuco, Brazil. We analyzed 93 isolates and we observed the presence of toxigenic gene in 20 (21.6%) samples, of which 11 (55.0%) were positive for tst gene, seven (35.0%) for the sec gene, and two (10.0%) for the seg gene. Of the 20 isolates amplified in PCR for the presence of the sec gene, seg and tst, 16 (80.0%) were positive for only one gene and four (20.0%) were positive for two genes (sec and tst). Of the 17 properties from which the samples originated, seven (41.2%) had positive samples for at least one of the genes sec, seg, and tst. This is the first record of the occurrence of the genes encoding the enterotoxin SEC and TST-1 in milk samples from cows with mastitis in the state of Pernambuco, Brazil.
DOI: 10.6084/m9.figshare.7510631
title: MORPHOMETRIC ASSESSMENT OF CANINE HEART WITHOUT MACROSCOPICALLY VISIBLE CHANGES CAUSED BY CARDIAC DISEASE

description: Abstract The purpose of this study was to collect data on the cardiac morphometry of thirty adult male and female dogs without macroscopic changes indicative of heart disease, regardless of animal's body weight and score. The dogs were weighed, grouped according to their body weight, and necropsied for a macroscopic evaluation of their cardiac measurements. The data obtained were included in a database. The following cardiac measures were evaluated: vertical and horizontal axes, weight, volume, thickness of the right and left atria and ventricles, and horizontal and vertical axes of the atrial and ventricular chambers. The heart weight was found to represent 0.6 to 1.1% of the animal's body weight. No differences were found in cardiac size and weight as a function of sex. The ratio between the vertical and horizontal cardiac axes was 1.11. The left ventricle was found to be 1.6 to threefold thicker than the right ventricle. The results were considered standard for dogs and can be used for macroscopic evaluations of cardiac hypertrophy or dilation.
DOI: 10.6084/m9.figshare.7510631.v1
title: MORPHOMETRIC ASSESSMENT OF CANINE HEART WITHOUT MACROSCOPICALLY VISIBLE CHANGES CAUSED BY CARDIAC DISEASE

description: Abstract The purpose of this study was to collect data on the cardiac morphometry of thirty adult male and female dogs without macroscopic changes indicative of heart disease, regardless of animal's body weight and score. The dogs were weighed, grouped according to their body weight, and necropsied for a macroscopic evaluation of their cardiac measurements. The data obtained were included in a database. The following cardiac measures were evaluated: vertical and horizontal axes, weight, volume, thickness of the right and left atria and ventricles, and horizontal and vertical axes of the atrial and ventricular chambers. The heart weight was found to represent 0.6 to 1.1% of the animal's body weight. No differences were found in cardiac size and weight as a function of sex. The ratio between the vertical and horizontal cardiac axes was 1.11. The left ventricle was found to be 1.6 to threefold thicker than the right ventricle. The results were considered standard for dogs and can be used for macroscopic evaluations of cardiac hypertrophy or dilation.
DOI: 10.6084/m9.figshare.7045868
title: PRE AND POST-COLOSTRAL DYNAMICS OF ERYTHRO-LEUKOGRAM, TOTAL PLASMA PROTEIN, AND FIBRINOGEN OF LAMBS

description: Abstract The aim of this study was to characterize the dynamics of some hematological parameters in healthy newborn lambs before and after colostrum intake. Blood samples of 28 newborn lambs were collected, determining the erythro-leukogram, the total plasma protein (TPP) and fibrinogen concentrations in pre and post-colostrum moments. The data were analyzed comparing the variability of the parameters between the two moments. The packed cell volume (P&lt;0.001), the hemoglobin concentration (P&lt;0.05) and the mean corpuscular volume (MCV) (P&lt;0.05) decreased after colostrum intake, while the mean corpuscular hemoglobin concentration (MCHC) increased (P&lt;0.01) at the post-colostrum moment. There was an increase in the total white blood cell count (P&lt;0.001) and in the absolute counts of segmented neutrophils (P&lt;0.05) and lymphocytes (P&lt;0.01) after colostrum intake. The TPP and fibrinogen concentrations also increased at the post-colostrum moment (P&lt;0.001 and P&lt;0.01, respectively). The erythro-leukogram, the TPP and fibrinogen concentrations vary considerably before and after colostrum intake, being modified according to the own colostrum intake and/or the adaptation of the physiological processes of the animal to the extrauterine life. Therefore, it is recommended that the interpretation of complete blood cell counts performed in newborn lambs be made based on the specific reference intervals for the neonatal period.
DOI: 10.6084/m9.figshare.7045868.v1
title: PRE AND POST-COLOSTRAL DYNAMICS OF ERYTHRO-LEUKOGRAM, TOTAL PLASMA PROTEIN, AND FIBRINOGEN OF LAMBS

description: Abstract The aim of this study was to characterize the dynamics of some hematological parameters in healthy newborn lambs before and after colostrum intake. Blood samples of 28 newborn lambs were collected, determining the erythro-leukogram, the total plasma protein (TPP) and fibrinogen concentrations in pre and post-colostrum moments. The data were analyzed comparing the variability of the parameters between the two moments. The packed cell volume (P&lt;0.001), the hemoglobin concentration (P&lt;0.05) and the mean corpuscular volume (MCV) (P&lt;0.05) decreased after colostrum intake, while the mean corpuscular hemoglobin concentration (MCHC) increased (P&lt;0.01) at the post-colostrum moment. There was an increase in the total white blood cell count (P&lt;0.001) and in the absolute counts of segmented neutrophils (P&lt;0.05) and lymphocytes (P&lt;0.01) after colostrum intake. The TPP and fibrinogen concentrations also increased at the post-colostrum moment (P&lt;0.001 and P&lt;0.01, respectively). The erythro-leukogram, the TPP and fibrinogen concentrations vary considerably before and after colostrum intake, being modified according to the own colostrum intake and/or the adaptation of the physiological processes of the animal to the extrauterine life. Therefore, it is recommended that the interpretation of complete blood cell counts performed in newborn lambs be made based on the specific reference intervals for the neonatal period.
DOI: 10.6084/m9.figshare.7509185
title: EFFECT OF DIFFERENT ENVIRONMENTAL ENRICHMENT TECHNIQUES IN CAPTIVITY OF Puma concolor

description: Abstract: The objective of this study was to evaluate the effects of the implementation of different environmental enrichment techniques on the behavior of Puma concolor kept in captivity. Five Puma concolor, four males and one female, belonging to the Municipal Zoo of Ribeirão Preto-SP, were used for this study. For purposes of environmental enrichment, vine and sisal balls and cardboard boxes containing beef or lemon grass and cinnamon were introduced into the enclosures of the felines. The behavioral data were submitted to descriptive analysis. Even in captivity, the animals showed territorial demarcation behavior, such as urinating and roaring when feeling threatened. In addition, there was a greater interest of the Puma concolor for the cardboard boxes with beef to the detriment of those that contained lemon grass and cinnamon. The cougars also showed to be entertained by the vine and sisal balls. These findings suggest success of the environmental enrichment strategies studied, which was evidenced by the interest of the felines in the different evaluated materials. In addition, the enrichment strategies used in this study represent simple and low-cost strategies that contribute to the well-being of Puma concolor and can be applied in other institutions that house these animals.
DOI: 10.6084/m9.figshare.7509185.v1
title: EFFECT OF DIFFERENT ENVIRONMENTAL ENRICHMENT TECHNIQUES IN CAPTIVITY OF Puma concolor

description: Abstract: The objective of this study was to evaluate the effects of the implementation of different environmental enrichment techniques on the behavior of Puma concolor kept in captivity. Five Puma concolor, four males and one female, belonging to the Municipal Zoo of Ribeirão Preto-SP, were used for this study. For purposes of environmental enrichment, vine and sisal balls and cardboard boxes containing beef or lemon grass and cinnamon were introduced into the enclosures of the felines. The behavioral data were submitted to descriptive analysis. Even in captivity, the animals showed territorial demarcation behavior, such as urinating and roaring when feeling threatened. In addition, there was a greater interest of the Puma concolor for the cardboard boxes with beef to the detriment of those that contained lemon grass and cinnamon. The cougars also showed to be entertained by the vine and sisal balls. These findings suggest success of the environmental enrichment strategies studied, which was evidenced by the interest of the felines in the different evaluated materials. In addition, the enrichment strategies used in this study represent simple and low-cost strategies that contribute to the well-being of Puma concolor and can be applied in other institutions that house these animals.