cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11351318
title: Protective effects of Ginger Essential Oil (GEO) against chemically-induced cutaneous inflammation

description: Abstract The inhibitory effects of ginger essential oil (GEO) on chemically-induced cutaneous inflammation were investigated. Three kind of experimental murine models, such as xylene-induced ear edema model, carrageenan-induced paw edema model and 2,4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis (ACD) model were exploited to evaluate the anti-inflammatory effect of GEO. Results showed that GEO lowered the ear swelling rate induced by xylene and decreased paw edema rate induced by carrageenan of mice with cutaneous inflammation significantly. Treatment with GEO significantly inhibited DNFB-induced ear swelling degree and rate, and alleviated the inflammatory symptoms of ear skin lesions of ACD model mice markedly. Treatment with GEO decreased the gene expression of the inflammatory cytokines of auricular tissues such as TLR-2, TLR-4, TNF-α, IFN-γ, IL-1β, IL-8, and increased the gene expression of cytokine IL-4 significantly. In conclusion, GEO has anti-inflammatory and protective effect on chemically-induced cutaneous inflammation.
DOI: 10.6084/m9.figshare.11351321
title: Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos

description: BACKGROUND The Yellow Fever (YF) vaccine is produced by the inoculation of embryonated chicken eggs with YF17DD virus on the ninth day of development. Full embryos are collected on the twelfth day of development for vaccine formulation. Skeletal muscle tissue is the main site where biosynthesis of viral particles occurs. OBJECTIVES This study aimed to analyse the experimental infection of skeletal muscle cells of chicken embryos by the 17DD Yellow Fever virus (YFV) in vivo and in vitro. METHODS Chicken embryos infected with YF17DD virus were analysed by immunofluorescence using confocal and super-resolution microscopes. Primary cultures of skeletal muscle cells of non-infected chicken embryos were evaluated for susceptibility and permissiveness to YF17DD virus using different protocols. This evaluation was performed based on morphological, viral titration, molecular biology, and colorimetric techniques. FINDINGS The present work phenotypically characterises embryonic chicken skeletal muscle cells as myogenic precursors expressing the Pax7 transcription factor in some cases. We demonstrated that these cells are susceptible to in vitro infection at different multiplicities of infection (MOIs), reproducing the same infection pattern observed in vivo. Furthermore, myogenic precursors and myoblasts are preferred infection targets, but establishment of infection does not depend on the presence of these cells. The peak of viral production occurred at 48 hpi, with decay occurring 72 hpi, when the cytopathic effect can be observed. MAIN CONCLUSIONS In conclusion, the primary culture of chicken skeletal muscle cells is a good model for studying muscle cells infected with YF17DD virus. This culture system displays satisfactory emulation of the in vitro phenomenon observed, contributing to our understanding of virus infection dynamics and leading to the development of alternative methods of vaccine production.
DOI: 10.6084/m9.figshare.11351327.v1
title: Trends of rainfall and temperature in Northeast Brazil

description: ABSTRACT For climate change scenarios, there is a high degree of complexity, with impacts on the future availability of water resources. In this context, studies related to changes in rainfall time series are essential in order to identify environmental vulnerability. The objective of this study was to analyze trends in the rainfall regime, number of rainy days and temperature for stations located at different continentality and altitude conditions in Northeast Brazil. Meteorological data of the Instituto Nacional de Meteorologia were used, being classified according to the location in relation to the continent: coastal strip (14 stations), strip of 150-300 km up to 300 m altitude (14 stations) and above 300 m (five stations), between 400 and 600 km from the coast up to 300 m (four stations) and above 300 m (eight stations). All the 45 stations used have a historical series with a data period of more than 30 years. The trend of rainfall and rainy days was obtained through the Mann-Kendall and regression analyses, at significance levels of 0.01 and 0.05, respectively. There were trends of reduction in the number of rainy days, in the coastal strip, as well as reduction in rainfall and rainy days, both in the strip of 150-300 km from the coast and in the Sertão region, with no significant effect of continentality in the strip of 400-600 km from the coast. For temperature, except for Maceió, AL, Brazil, there is a trend of increase in near future.
DOI: 10.6084/m9.figshare.11351309
title: Potential of pepper plant accessions for ornamental purposes using diallel analysis

description: Abstract: The genus Capsicum is cultivated in various parts of the world, with C. annuum showing high diversity and potential for ornamental purposes and genetic improvement. The aim of this work was to evaluate the combining ability and heterosis of seedling and plant traits in accessions of ornamental pepper and their hybrids. Seven accessions of C. annuum were handcrossed and twelve quantitative plant traits were evaluated. The data were subjected to analysis of variance, the Scott-Knott test and diallel analysis using Griffing’s method I (fixed model). Heterosis and its components were also determined. Non-additive effects were found for seedling height, cotyledonous leaf length and width; stem diameter and chlorophyll b content. All other traits showed predominance of additive effects. Heterosis effects were found for some hybrids and UFPB134 x UFPB137, UFPB134 x UFPB390, UFPB134 x UFPB004 and UFPB099 x UFPB004 showed seedlings that are more vigorous.
DOI: 10.6084/m9.figshare.11351315
title: AN INVESTIGATION OF THE HEAT TRANSFER PERFORMANCE OF DUAL IMPROVED INTERMIG IMPELLERS IN A STIRRED TANK WITH AN INNER HEATING COIL

description: Abstract The heat transfer process in a stirred tank of diameter T=0.5m equipped with dual-layer improved Intermig impellers and helical coils was investigated by experiment and numerical simulation methods. The temperature field, the temperature boundary layer lateral to the coil and the heat transfer coefficient were measured at different rotational speeds. The standard k-ε turbulence model and multiple reference frames combined with a sliding mesh method were adopted in the numerical simulation. The results show that the temperature errors between numerical simulation and experimental measurement were within 2K. The temperature in the stirred tank gradually rose from top to bottom and inside to outside, and the maximum temperature difference was within 2K. The average thickness of the temperature boundary layer outside the helical coil is 3.66 mm. According to the experiments and numerical simulations, the heat transfer coefficient correlations, including Nu and Re, Nu and ε of the helical coil outer side, were obtained, and the trends of heat transfer coefficients are consistent and regular. The correlation of the heat transfer coefficient lateral to the coil was acquired from the experimentally measured data. The research results can serve as a guide for the design and engineering application of mass and heat transfer processes in stirred tanks with improved Intermig impellers.
DOI: 10.6084/m9.figshare.11351318.v1
title: Protective effects of Ginger Essential Oil (GEO) against chemically-induced cutaneous inflammation

description: Abstract The inhibitory effects of ginger essential oil (GEO) on chemically-induced cutaneous inflammation were investigated. Three kind of experimental murine models, such as xylene-induced ear edema model, carrageenan-induced paw edema model and 2,4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis (ACD) model were exploited to evaluate the anti-inflammatory effect of GEO. Results showed that GEO lowered the ear swelling rate induced by xylene and decreased paw edema rate induced by carrageenan of mice with cutaneous inflammation significantly. Treatment with GEO significantly inhibited DNFB-induced ear swelling degree and rate, and alleviated the inflammatory symptoms of ear skin lesions of ACD model mice markedly. Treatment with GEO decreased the gene expression of the inflammatory cytokines of auricular tissues such as TLR-2, TLR-4, TNF-α, IFN-γ, IL-1β, IL-8, and increased the gene expression of cytokine IL-4 significantly. In conclusion, GEO has anti-inflammatory and protective effect on chemically-induced cutaneous inflammation.
DOI: 10.6084/m9.figshare.11351324.v1
title: Mineral and centesimal contents, antioxidant activity and antimicrobial action of phenolic compounds from Eugenia Brasiliensis Lam. Pulp

description: Abstract Phenolic compounds (PC) have presented antioxidant potential as well as antimicrobial action against several bacteria. This study aimed to extract PC from grumixama (Eugenia brasiliensis Lam.) pulp using solid phase extraction (SPE), then quantify the PC by Folin-Ciocalteu assay, and to evaluate the antioxidant activity (AA) from PC by DPPH, ABTS and FRAP methods as well as its antimicrobial activity by plate diffusion assay (PDA) and Minimal Inhibitory Concentration (MIC) assays. Additionally, the centesimal composition of the fruit and its mineral content by Total Reflection X-Ray Fluorescence Spectroscopy were determined. The fruit is a good source of PC which presented a higher contribution on AA from pulp than NPHRC. Calcium was the trace element in highest concentration in the pulp, followed by iron. PC from grumixama inhibited the growth of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa but not Escherichia coli and Listeria monocytogenes in the PDA. PC showed antimicrobial activity against the five microorganisms tested in the MIC assay, in which S. aureus and B. cereus were the most sensitive. This study highlights the antioxidant and antimicrobial potential of Eugenia brasiliensis Lam. as natural agent in the development of functional foods and nutraceuticals.
DOI: 10.6084/m9.figshare.11351306
title: Microstructural Evolution and Phase Transformation of a Ti-5Nb-5Al Alloy During Annealing Treatment

description: The microstructural evolution and phase transformation of a Ti-5Nb-5Al alloy during isothermal annealing treatment were studied in this paper. The microstructural evolution was analyzed by X-ray diffraction (XRD), scanning electron microscope (SEM) equipped with X-ray energy dispersive spectroscopy (EDS) and electron backscattering diffraction (EBSD). The first-principle calculation by density functional theory method was performed to analyze the effect of niobium diffusion on the α to β transformation. The results showed that the α phase initially grew by merging neighboring grains and then by boundary splitting with increasing the solution time below the β-transus temperature. However, the α phase disappeared absolutely above the β-transus temperature. According to the thermodynamic analysis by DFT calculations, the diffusion of niobium from β to α phase can promote the α to β transformation. After quenching in water, the acicular α’ phase precipitated from β matrix with an orientation relationship of {110}bcc||{0001}hcp and &lt;111&gt;bcc||&lt;1120&gt;hcp, and thus increased the hardness of the alloy.
DOI: 10.6084/m9.figshare.11351309.v1
title: Potential of pepper plant accessions for ornamental purposes using diallel analysis

description: Abstract: The genus Capsicum is cultivated in various parts of the world, with C. annuum showing high diversity and potential for ornamental purposes and genetic improvement. The aim of this work was to evaluate the combining ability and heterosis of seedling and plant traits in accessions of ornamental pepper and their hybrids. Seven accessions of C. annuum were handcrossed and twelve quantitative plant traits were evaluated. The data were subjected to analysis of variance, the Scott-Knott test and diallel analysis using Griffing’s method I (fixed model). Heterosis and its components were also determined. Non-additive effects were found for seedling height, cotyledonous leaf length and width; stem diameter and chlorophyll b content. All other traits showed predominance of additive effects. Heterosis effects were found for some hybrids and UFPB134 x UFPB137, UFPB134 x UFPB390, UFPB134 x UFPB004 and UFPB099 x UFPB004 showed seedlings that are more vigorous.
DOI: 10.6084/m9.figshare.11351321.v1
title: Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos

description: BACKGROUND The Yellow Fever (YF) vaccine is produced by the inoculation of embryonated chicken eggs with YF17DD virus on the ninth day of development. Full embryos are collected on the twelfth day of development for vaccine formulation. Skeletal muscle tissue is the main site where biosynthesis of viral particles occurs. OBJECTIVES This study aimed to analyse the experimental infection of skeletal muscle cells of chicken embryos by the 17DD Yellow Fever virus (YFV) in vivo and in vitro. METHODS Chicken embryos infected with YF17DD virus were analysed by immunofluorescence using confocal and super-resolution microscopes. Primary cultures of skeletal muscle cells of non-infected chicken embryos were evaluated for susceptibility and permissiveness to YF17DD virus using different protocols. This evaluation was performed based on morphological, viral titration, molecular biology, and colorimetric techniques. FINDINGS The present work phenotypically characterises embryonic chicken skeletal muscle cells as myogenic precursors expressing the Pax7 transcription factor in some cases. We demonstrated that these cells are susceptible to in vitro infection at different multiplicities of infection (MOIs), reproducing the same infection pattern observed in vivo. Furthermore, myogenic precursors and myoblasts are preferred infection targets, but establishment of infection does not depend on the presence of these cells. The peak of viral production occurred at 48 hpi, with decay occurring 72 hpi, when the cytopathic effect can be observed. MAIN CONCLUSIONS In conclusion, the primary culture of chicken skeletal muscle cells is a good model for studying muscle cells infected with YF17DD virus. This culture system displays satisfactory emulation of the in vitro phenomenon observed, contributing to our understanding of virus infection dynamics and leading to the development of alternative methods of vaccine production.
DOI: 10.6084/m9.figshare.11351306.v1
title: Microstructural Evolution and Phase Transformation of a Ti-5Nb-5Al Alloy During Annealing Treatment

description: The microstructural evolution and phase transformation of a Ti-5Nb-5Al alloy during isothermal annealing treatment were studied in this paper. The microstructural evolution was analyzed by X-ray diffraction (XRD), scanning electron microscope (SEM) equipped with X-ray energy dispersive spectroscopy (EDS) and electron backscattering diffraction (EBSD). The first-principle calculation by density functional theory method was performed to analyze the effect of niobium diffusion on the α to β transformation. The results showed that the α phase initially grew by merging neighboring grains and then by boundary splitting with increasing the solution time below the β-transus temperature. However, the α phase disappeared absolutely above the β-transus temperature. According to the thermodynamic analysis by DFT calculations, the diffusion of niobium from β to α phase can promote the α to β transformation. After quenching in water, the acicular α’ phase precipitated from β matrix with an orientation relationship of {110}bcc||{0001}hcp and &lt;111&gt;bcc||&lt;1120&gt;hcp, and thus increased the hardness of the alloy.
DOI: 10.6084/m9.figshare.11351315.v1
title: AN INVESTIGATION OF THE HEAT TRANSFER PERFORMANCE OF DUAL IMPROVED INTERMIG IMPELLERS IN A STIRRED TANK WITH AN INNER HEATING COIL

description: Abstract The heat transfer process in a stirred tank of diameter T=0.5m equipped with dual-layer improved Intermig impellers and helical coils was investigated by experiment and numerical simulation methods. The temperature field, the temperature boundary layer lateral to the coil and the heat transfer coefficient were measured at different rotational speeds. The standard k-ε turbulence model and multiple reference frames combined with a sliding mesh method were adopted in the numerical simulation. The results show that the temperature errors between numerical simulation and experimental measurement were within 2K. The temperature in the stirred tank gradually rose from top to bottom and inside to outside, and the maximum temperature difference was within 2K. The average thickness of the temperature boundary layer outside the helical coil is 3.66 mm. According to the experiments and numerical simulations, the heat transfer coefficient correlations, including Nu and Re, Nu and ε of the helical coil outer side, were obtained, and the trends of heat transfer coefficients are consistent and regular. The correlation of the heat transfer coefficient lateral to the coil was acquired from the experimentally measured data. The research results can serve as a guide for the design and engineering application of mass and heat transfer processes in stirred tanks with improved Intermig impellers.
DOI: 10.6084/m9.figshare.11351291
title: Agronomic yield and quality of industrial tomatoes under NPK doses

description: ABSTRACT High doses of nitrogen, phosphorus and potassium in irrigated systems may lead to an increase in the productivity of industrial tomatoes, but may also alter the final quality of the fruits. The objective of this was to evaluate the agronomic yield and post-harvest quality of industrial tomatoes, under NPK doses for climatic conditions of Northern Minas Gerais state, Brazil. The study was conducted in the city of Jaíba, MG, Brazil, in randomized block design with four repetitions in a 3 x 3 factorial scheme, consisting of three doses of N (90, 135 and 180 kg ha-1), of P (270, 405 and 540 kg ha-1) and of K (225, 337 and 450 kg ha-1), corresponding to 50, 75 and 100% of the fertilization recommendation; and three tomato hybrids: BRS Sena, Heinz 9553 and BHN 0574. The following variables were evaluated: number of bunches, number and weight of fruits, productivity and fertilizer efficiency index; besides the physico-chemical characteristics: firmness, soluble solids concentration, pH and pulp industrial yield. The hybrid Heinz 9553 showed higher weight, number of fruits, productivity and industrial yield at doses of 75 and 100% of NPK recommendation. The fertilizer doses did not interfere on the soluble solids, pH and fruit firmness of the three hybrids. The hybrid BRS Sena was not influenced by the increase of NPK and there was lower weight, number of fruits, productivity and fertilization efficiency. The hybrid BHN and Heinz hybrids can be indicated for cultivation at doses of 100 and 75% of NPK recommendation, because they promote higher agronomic yield and do not alter the post-harvest quality of the tomatoes.
DOI: 10.6084/m9.figshare.11351294
title: Functional capacity and quality of life of hospitalized octogenarians

description: ABSTRACT Objective: To evaluate the ability to perform activities of daily living (ADL) and to correlate functional capacity with quality of life (QoL) of hospitalized octogenarians. Method: A cross-sectional study with 128 patients using the quality of life instruments WHOQOL-OLD and WHOQOL-BREF and the Katz Scale. Results: The majority of patients was fully dependent; patients with higher schooling had less independence; older adults with partial dependence and independence had higher scores in perceived QoL; in the domains of autonomy, past, present and future activities; and better overall QoL than those with full dependence. Partially dependent patients had higher scores in the death and dying domain than independent patients. Conclusion: Older adults’ QoL is associated with the maintenance of autonomy and functional capacity. Older adults experience a health decline during the hospitalization process, due to the limitation in performing their ADL and lack of encouragement by the health team.
DOI: 10.6084/m9.figshare.11351297
title: Functional capacity assessment of long-lived older adults from Amazonas

description: ABSTRACT Objective: To evaluate the functional capacity of long-lived older adults from Amazonas. Method: A cross-sectional epidemiological study was carried out with 116 older adults aged 80 years or older, registered in a primary health care unit in Belém, in the state of Pará, Brazil. The Functional Independence Measure (FIM) was used for functional capacity assessment and the Mini-Mental State Examination (MMSE) for cognitive screening. Univariate and bivariate analyses were carried out, in addition to the Pearson’s chi-square test. Results: The older adults presented modified independence in the self-care, sphincter control and locomotion dimensions, and needed supervision for mobility/transfers. In mobility, men presented complete independence. Modified independence was found in the 80-89 age group. It was observed that, the lower the education level, the worse the cognitive performance. Conclusion: In spite of their advanced age, long-lived older adults still present functional capacity for activities of daily living, even though they required supervision for high energy expenditure tasks, such as mobility and transfers.
DOI: 10.6084/m9.figshare.11351300
title: Optimization of extraction process for efficient imino acids recovery and purification from low-value sea cucumber

description: Abstract For high recoveries and purities of L-proline (L-Pro) and L-hydroxyproline (L-Hyp) from the low-value sea cucumber Acaudina leucoprocta, the extraction process was systematically investigated and optimized. The results indicated that, in decoloration of A. leucoprocta hydrolysate, activated carbon powdered W660 was more suitable due to the low addition amount and short treatment time compared with macroporous resin HZ816 offering comparable decoloration ratio and imino acids recovery yields. For oxidative deamination, HCl-HNO3 exhibited better performance than NaNO2 with complete primary amino acids broken-down and fewer imino acids loss. After decoloration and oxidative deamination under their optimized conditions, an extraction solution was obtained with L-Pro and L-Hyp recovery yields of 76.3% and 78.8% from A. leucoprocta hydrolysate, which was directly used for separation of L-Pro and L-Hyp with ion-exchange chromatograph. This work may be referenced for the large-scale L-Pro and L-Hyp production from other low-value materials.