DOI: 10.5061/dryad.v72m2
title: Data from: Characterization of transcriptomes from sexual and asexual lineages of a New Zealand snail (Potamopyrgus antipodarum)

description: Understanding the evolution and maintenance of sexual reproduction is one
 of the central challenges of evolutionary biology, yet we know very little
 about how sex influences molecular evolution. The New Zealand freshwater
 snail Potamopyrgus antipodarum is ideally suited to address this knowledge
 gap because obligately sexual individuals often coexist with multiple
 independently derived obligately asexual lineages. This unusual situation
 allows direct comparisons both between sexual and asexual P. antipodarum
 and across populations that differ in the relative frequency of sexual
 individuals. As such, P. antipodarum has received a great deal of
 attention as a model system for the maintenance of sex in nature and is
 also used as a model for environmental toxicology and biological
 invasions. Molecular genetic resources for P. antipodarum will thus be
 useful to investigators in a variety of biological fields. We used 454
 sequencing of cDNA libraries to generate transcriptomes from two sexual
 and two asexual P. antipodarum lineages. A de novo assembly of 116.7 Mb of
 sequence reads produced 41 396 contigs, and sequence similarity-based Gene
 Ontology annotations were obtained for 3740 contigs. We detected 408 315
 SNP loci and 7315 microsatellite loci, which together represent the first
 genome-scale resource available for P. antipodarum.
description: P. antipodarum 454 read
 sequencesRead sequences from 454
 sequencing of two sexual and two asexual lineages of the New Zealand snail
 Potamopyrgus antipodarum. RNA was extracted from four
 independently-isolated P. antipodarum lineages descended from wild-caught
 females originally sampled from three New Zealand South Island lakes: Lake
 Alexandrina (one sexual diploid lineage and one asexual triploid lineage),
 Lady Lake (sexual diploid), and Lake Mapourika (asexual triploid). Each
 lineage was represented by four arbitrarily selected adult females. For
 details on extraction and sequencing efforts, see the associated
 publication.P_antipodarum_454_reads_FASTA.tar.gzP. antipodarum contig annotationsBLAST results and Gene Ontology annotations of contig sequences from transcriptome sequencing of two sexual and two asexual lineages of New Zealand snail Potamopyrgus antipodarum. BLAST annotations were made using the Tera-BLASTX implementation of the BLASTX algorithm. GO annotations were made using the software BLAST2GO; BLAST2GO was provided with BLASTX results of a search against the NCBI ns database, and default values were used for all cutoff parameters.P_antipodarum_annotations.tar.gzP. antipodarum contig assembliesAssemblies of reads from the transcriptome sequencing of two sexual and two asexual lineages of New Zealand snail Potamopyrgus antipodarum. Read assembly was carried out using the sotware MIRA3. The assemblies are in .ace format.P_antipodarum_contig_assemblies.tar.gzP. antipodarum contig sequencesAssembled contig sequences from the 454 transcriptome sequencing of two sexual and two asexual lineages of New Zealand snail Potamopyrgus antipodarum. Contig sequences were produced by the software MIRA3.P_antipodarum_contig_sequences.fastaP. antipodarum microsatellite lociThese files describe the locations (flanking sequence) of putative microsatellite loci in the New Zealand snail Potamopyrgus antipodarum. The loci were identified by scanning contig sequences from a 454 transcriptome sequencing experiment with the software MSATCOMMANDER.P_antipodarum_microsatellites.tar.gzP. antipodarum SNP lociLocations of SNP loci from 454 transcriptome sequencing of two sexual and two asexual lineages the New Zealand snail Potamopyrgus antipodarum. Positions of SNPs are relative to contig sequences assembled in this sequencing experiment. The software MIRA3 was used for both read alignment (i.e., contig generation) and SNP discovery. Both padded and unpadded positions are given.P_antipodarum_SNPs.tar.gz