cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1466767.v29
title: Program for keeping into account the solar reflections from obstructions in ESP-r on the basis of the irradiance ratios

description: Modish is a program for bringing into account the reflective effect of the solar obstructions on solar gains in an ESP-r building model on the basis of irradiance ratios. Those ratios are obtained combining the direct radiation on a surface and the total radiation on it calculated by the means of Radiance. Modish is written in Perl and can be installed from the CPAN with the command: sudo cpanm Sim::OPT::Modish
DOI: 10.6084/m9.figshare.1464988.v1
title: Gel-filtration chromatography data for L35Ae samples

description: Gel-filtration chromatography data for L35Ae samples (3.5-3.7 mg/ml) and molecular mass standards at room temperature (Superdex 75 10/300 GL column). The elution profiles of L35Ae samples are shown in the inset. The open circles corresponding to elution times of the mass standards were described by linear function (solid line). The apparent molecular masses of L35Ae samples were determined by comparison of their elution times with the calibration line, as indicated by dashed arrowed lines.
DOI: 10.6084/m9.figshare.1464990.v1
title: flow cytometry data

description: Flow cytometry data for the HEK293 cells versus protein concentration. HEK293 cells were incubated with 6´His-tagged L35Ae or anti-hCCR7 Fab17 used as a negative control, followed by immunostaining. FACS buffer contained PBS, 150 mM NaCl, pH 7.0, 1-1.5% calf serum, and 0.01% NaN3. R-Phycoerythrin (PE) fluorescence was excited at 532 nm and detected at 580-583 nm.
DOI: 10.6084/m9.figshare.1464992.v2
title: Stability of L35Ae samples towards chemical denaturation

description: GuHCl-induced changes in position of tryptophan fluorescence spectrum maximum of L35Ae. Theoretical fits to the experimental data using Boltzmann function were made. Buffer contained PBS, 150 mM NaCl, pH 7.0. Protein concentration was 3 µM.
DOI: 10.17632/3t8f68pphv.1
title: 53BP1-ACLY-SLBP-coordinated activation of replication-dependent histone homeostasis maintains genomic integrity .

description: Original imaging data and dataset of graphs.
DOI: 10.6084/m9.figshare.1464987
title: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm ( 10X)

description: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm.Buffer conditions: PBS, 150 mM NaCl, pH 7.0.
DOI: 10.6084/m9.figshare.1464986
title: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm

description: GuHCl-induced unfolding of rWT L35Ae samples followed by fluorescence intensity at 314 nm.Buffer conditions: PBS, 150 mM NaCl, pH 7.0.
DOI: 10.6084/m9.figshare.1464990.v2
title: Analysis of L35Ae interaction with HEK293 cells

description: Flow cytometry data for the HEK293 cells versus protein concentration. HEK293 cells were incubated with 6´His-tagged L35Ae or anti-hCCR7 Fab17 used as a negative control, followed by immunostaining. FACS buffer contained PBS, 150 mM NaCl, pH 7.0, 1-1.5% calf serum, and 0.01% NaN3. R-Phycoerythrin (PE) fluorescence was excited at 532 nm and detected at 580-583 nm.
DOI: 10.6084/m9.figshare.1465014.v5
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.6084/m9.figshare.1464990.v4
title: L35Ae interaction with HEK293 cells

description: HEK293 cells were incubated with 6xHis-tagged L35Ae or anti-hCCR7 Fab17 used as a negative control, followed by immunostaining and flow cytometry analysis. FACS buffer contained PBS, 150 mM NaCl, pH 7.0, 1-1.5% calf serum, and 0.01% NaN3. R-Phycoerythrin fluorescence was excited at 532 nm and detected at 580-583 nm.
DOI: 10.6084/m9.figshare.1466767.v30
title: Program for keeping into account the solar reflections from obstructions in ESP-r on the basis of the irradiance ratios

description: Modish is a program for bringing into account the reflective effect of the solar obstructions on solar gains in an ESP-r building model on the basis of irradiance ratios. Those ratios are obtained combining the direct radiation on a surface and the total radiation on it calculated by the means of Radiance. Modish is written in Perl and can be installed from the CPAN with the command: sudo cpanm Sim::OPT::Modish
DOI: 10.6084/m9.figshare.1464986.v1
title: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm

description: GuHCl-induced unfolding of rWT L35Ae samples followed by fluorescence intensity at 314 nm.Buffer conditions: PBS, 150 mM NaCl, pH 7.0.
DOI: 10.6084/m9.figshare.1464987.v1
title: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm ( 10X)

description: GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm.Buffer conditions: PBS, 150 mM NaCl, pH 7.0.
DOI: 10.6084/m9.figshare.1464990.v3
title: Analysis of L35Ae interaction with HEK293 cells

description: HEK293 cells were incubated with 6xHis-tagged L35Ae or anti-hCCR7 Fab17 used as a negative control, followed by immunostaining and flow cytometry analysis. FACS buffer contained PBS, 150 mM NaCl, pH 7.0, 1-1.5% calf serum, and 0.01% NaN3. R-Phycoerythrin fluorescence was excited at 532 nm and detected at 580-583 nm.
DOI: 10.6084/m9.figshare.1465014.v6
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.17632/fmhtdcs5mf.1
title: Major and Trace Elements in Moldavian Soils and Fruits by Epithermal Neutron Activation Analysis

description: Original data concerning the content of 39 elements (Na. Mg. Al. Ca. Si. K. Mn. Fe Sc. Ti. V. Cr. Co. Ni. Zn. As. Br. Rb. Sr. Zr. Mo. Cd. Sb. Cs. Ba. La. Ce. Nd. Sm. Eu. Tb. Dy. Tm. Yb. Hf. Ta. W. Th and U) as determined by Epithermal Neutron Activation Analysis in soil and in three sorts of fruits: apple. grapes and plums. all collected from four orchards located in the Republic of Moldova
DOI: 10.6084/m9.figshare.1464985
title: Stability of rWT L35Ae and L35Ae 10X towards chemical denaturation

description: GuHCl-induced changes in position of tryptophan fluorescence spectrum maximum of L35Ae. Buffer contained PBS, 1500 mM NaCl, pH 7.0. Protein concentration was 3 µM.
DOI: 10.6084/m9.figshare.1465014.v7
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.6084/m9.figshare.1466767.v31
title: Program for keeping into account the solar reflections from obstructions in ESP-r on the basis of the irradiance ratios

description: Modish is a program for bringing into account the reflective effect of solar obstructions on solar gains in an ESP-r building model on the basis of irradiance ratios combining the direct radiation on a surface, as calculated by ESP-r, and the total radiation on the same surface, as calculated by the means of Radiance. Modish is written in Perl and can be installed from the CPAN with the command: sudo cpanm Sim::OPT::ModishWhen Modish is installed, its documentation can be opened with the command:perldoc Sim::OPT::Modish<br>
DOI: 10.6084/m9.figshare.1464985.v2
title: Stability of rWT L35Ae and L35Ae 10X towards chemical denaturation

description: GuHCl-induced changes in position of tryptophan fluorescence spectrum maximum of L35Ae. Buffer contained PBS, 1500 mM NaCl, pH 7.0. Protein concentration was 3 µM.
DOI: 10.6084/m9.figshare.1464985.v1
title: L35AE

description: GuHCl-induced changes in position of tryptophan fluorescence spectrum maximum of L35Ae. Buffer contained PBS, 1500 mM NaCl, pH 7.0. Protein concentration was 3 µM.
DOI: 10.6084/m9.figshare.1466767.v32
title: Program for keeping into account the solar reflections from obstructions in ESP-r on the basis of the irradiance ratios

description: Modish is a program for bringing into account the reflective effect of solar obstructions on solar gains in an ESP-r building model on the basis of irradiance ratios combining the direct radiation on a surface, as calculated by ESP-r, and the total radiation on the same surface, as calculated by the means of Radiance. Modish is written in Perl and can be installed from the CPAN with the command: sudo cpanm Sim::OPT::ModishWhen Modish is installed, its documentation can be opened with the command:perldoc Sim::OPT::Modish<br>
DOI: 10.6084/m9.figshare.1464979
title: Study 2 Data Seeing Red

description: Data for PLOSONE Manuscript "Seeing Red: Anger Increases How Much Republican Identification Predicts Partisan Attitudes and Perceived Polarization"
DOI: 10.6084/m9.figshare.1465014.v8
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.17632/szg8nkhpgz.1
title: A data set of proximity of mandibular third molar roots with Inferior Alveolar Canal.

description: OPG’s are inexpensive, low radiation and easily available in smaller dental units and hospitals also however, a real three-dimensional picture and buccolingual relationship is unknown. Dearth of studies on third molar roots and inferior alveolar canal in Nepalese population persuaded us to carry out this study to describe the proximity of mandibular third molar roots to the inferior alveolar canal and nerve. A single centre cross-sectional study was designed with sample size of 475 patients collected from July 2017 to July 2019, at the department of Oral and maxillofacial surgery of College of Medical Sciences and Teaching Hospital (COMS-TH), Bharatpur, Nepal. Ethical approval was obtained from institutional review committee of COMS-TH. All the patients who reported the signs and symptoms of third molar pain and difficulty were included. Pregnant patients and those who didn’t provide consent were excluded. Orthopantomogram (OPG) of patients were retrieved from imaging software records. Baseline demographic data was obtained from patient’s entry in OPG imaging software program (Orthoralix Vixwin software) and signs of proximity of mandibular third molar roots to inferior alveolar canal as described by Rood and Shebab were ascertained. The seven radiographic signs were observed on digitally magnified OPG’s using Orthoralix Vixwin software (Gendex, USA) and presence or absence of signs were entered. Presence of simultaneous multiple signs were entered. Explicit prior criteria have been used to designate each of the seven panoramic radiographic signs as “positive” when present or “negative” when absent. All observations and demographic datas were entered in Microsoft excel and descriptive analysis was performed with SPSS Version 20. Frequency was used to describe presence of radiographic signs and means were used to describe demographic variables. Cross tabulation was done to analyse the pattern of combination of signs. One or more radiographic signs were observed in 49.6% of mandibular third molars. Interruption of white line followed by narrowing of canal and darkening of roots was observed in decreasing order of frequency and no statistically significant association between sex, age and side of impacted third molar with presence of radiographic signs. Absence of any radiographic sign was observed in 245 cases (51.6%). When only one sign was observed (n=71), the most common sign was, interruption of white line of canal followed by deflection of roots. The next most common pattern of combination was 3 signs (n=60) followed by two signs together (n=55).
DOI: 10.6084/m9.figshare.1465014.v9
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.6084/m9.figshare.1464978
title: Study 1 Data Seeing Red

description: Data for PLOSONE Manuscript "Seeing Red: Anger Increases How Much Republican Identification Predicts Partisan Attitudes and Perceived Polarization"
DOI: 10.6084/m9.figshare.1464979.v2
title: Study 2 Data Seeing Red

description: Data for PLOSONE Manuscript "Seeing Red: Anger Increases How Much Republican Identification Predicts Partisan Attitudes and Perceived Polarization"
DOI: 10.6084/m9.figshare.1466767.v33
title: Program for keeping into account the solar reflections from obstructions in ESP-r on the basis of the irradiance ratios

description: Modish is a program for bringing into account the reflective effect of solar obstructions on solar gains in an ESP-r building model on the basis of irradiance ratios combining the direct radiation on a surface, as calculated by ESP-r, and the total radiation on the same surface, as calculated by the means of Radiance. Modish is written in Perl and can be installed from the CPAN with the command: sudo cpanm Sim::OPT::ModishWhen Modish is installed, its documentation can be opened with the command:perldoc Sim::OPT::Modish<br>
DOI: 10.6084/m9.figshare.1465014.v11
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.
DOI: 10.6084/m9.figshare.1464976
title: Thermal denaturation of L35Ae samples, monitored by DSC

description: The temperature dependencies of excess specific heat capacity for rWT L35Ae and L35Ae 10X, fitted by a single Gaussian function (J/(g*K) units). Protein concentrations were 0.65 mg/ml and 1.5 mg/ml, for rWT L35Ae and L35Ae 10X samples, respectively. Buffer conditions: 20 mM H3BO3–NaOH, 300 mM NaCl, pH 8.5. Heating rate was 1 K/min.
DOI: 10.6084/m9.figshare.1464979.v1
title: Study 1 Data

description: Data for PLOSONE Manuscript "Seeing Red: Anger Increases How Much Republican Identification Predicts Partisan Attitudes and Perceived Polarization"
DOI: 10.6084/m9.figshare.1465014.v10
title: Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

description: Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.