DOI: 10.5061/dryad.8h5p7p00
title: Data from: The phylogenetics of Anguillicolidae (Nematoda: Anguillicolidea), swimbladder parasites of eels

description: BACKGROUND: Anguillicolidae Yamaguti, 1935 is a family of nematode
 parasites infecting fresh-water eels of the genus Anguilla, with five
 species in the genera Anguillicola and Anguillicoloides. Anguillicolidae
 is part of Spirurina, a diverse clade made up of only animal parasites.
 Anguillicoloides crassus is of particular importance, as it has recently
 spread from its endemic range in the Eastern Pacific to Europe and North
 America, where it poses a significant threat to new, naïve hosts such as
 the economic important eel species Anguilla anguilla and Anguilla
 rostrata. The Anguillicolidae are therefore all potentially invasive taxa,
 but the relationships of the described species remain unclear. RESULTS: We
 generated an extensive DNA sequence dataset from three loci (the 5'
 one-third of the nuclear small subunit ribosomal RNA, the D2-D3 region of
 the nuclear large subunit ribosomal RNA and the 5' half of the
 mitochondrial cytochrome c oxidase I gene) for the five species of
 Anguillicolidae and used this to investigate species and generic
 boundaries within the family, and the relationship of Anguillicolidae to
 other spirurine nematodes. Neither nuclear nor mitochondrial sequences
 supported monophyly of the genera Anguillicola and Anguillicoloides.
 Genetic diversity within the African species Anguillicoloides papernai was
 suggestive of cryptic taxa, as was the finding of divergent lineages of
 Anguillicoloides novaezelandiae in New Zealand and Tasmania. Phylogenetic
 analysis of the Spirurina grouped the Anguillicolidae together with
 members of the Gnathostomatidae and Seuratidae. CONCLUSIONS: The
 Anguillicolidae is part of a complex radiation of vertebrate parasitic
 nematodes with wide host diversity (cartilaginous and bony fish, reptiles
 and mammals), most closely related to other parasites of marine
 vertebrates that also have complex lifecycles. Molecular analyses do not
 support the recent division of Anguillicolidae into two genera. The
 described species may hide cryptic taxa, identified here by DNA taxonomy,
 and this DNA barcoding approach may assist in tracking species invasions.
 The propensity for host switching, and thus the potential for invasive
 behaviour, is found in A. crassus, A. novaezelandiae and A. papernai, and
 thus may be common to the group.
description: Phylogeny of the
 Anguillicolidae Supplementary DataSequence data files and
 keys thereto as a single compressed file, expandable to a folder
 containing: Cytochrome oxidase 1 data, COX1.haplotypes.nex, NEXUS format
 file of aligned COXI haplotype sequences, COX1.haplotypes.labels.txt, text
 file describing the assignment of individual COXI sequences to
 hapolotypes, COX1.haplotypes.crassus.nex, NEXUS format file of aligned A.
 crassus COXI haplotype sequences, COX1.haplotypes.crassus.labels.txt, text
 file describing the assignment of individual A. crassus COXI sequences to
 hapolotypes, COX1.nex, NEXUS format file of all aligned COXI haplotype
 sequences, Nuclear large subunit ribosomal RNA data, 28_S.haplotypes.nex
 NEXUS format file of aligned nLSU or 28S haplotype sequences, 28_S.nex,
 NEXUS format file of all aligned nLSU or 28S haplotype sequences, Nuclear
 small subunit ribosomal RNA data, 18_S.haplotypes.nex, NEXUS format file
 of aligned nSSU or 18S haplotype sequences, 18_S.spirurina_B.nex, NEXUS
 format file of aligned nSSU or 18S haplotype sequences, 18_S.nex, NEXUS
 format file of all aligned nSSU or 18S haplotype
 sequences