cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.29617871.v1
title: Supplementary Material for: Allometric Brain Development and Neuroecological Adaptations in Juvenile and Adult Raja polistigma

description: Chondrichthyans represent some of the earliest diverging lineages of jawed vertebrates, making them valuable models for studying vertebrate evolution. Many studies have explored how variations in brain anatomy relate to behaviour and ecological roles. This study examines the postnatal brain development and neuroecological adaptations in the speckled skate, Raja polystigma, which is an endemic species of the Mediterranean Sea, an understudied biodiversity hotspot for Chondrichthyan’ brain evolution. Its distinct life-history traits, including bathymetric segregation and shifts in feeding habits, made it a valuable model for studying how ecological factors shape brain development in benthic cartilaginous fishes. We focused on the allometry between brain mass and body mass, as well as the relative size of specific brain regions and the brain mass. Our analysis shows that as body mass increases, brain growth slows down relative to body growth, which may be linked to the skate reaching adulthood and sexual maturity. We also found that certain brain regions, like the olfactory bulbs, medulla oblongata, and cerebellum grow faster than the rest of the brain, suggesting these areas enhance sensory and motor functions as the skate grows. In contrast, the optic tectum tends to grow slower, possibly indicating a reduced reliance on vision in adults. The telencephalon and diencephalon grow at the same rate as the rest of the brain, which may support consistent cognitive and sensory functions throughout postnatal development. These findings help us to understand how the species adapts to its environment, with important implications for sensory specialization during its growth.
DOI: 10.6084/m9.figshare.29617874
title: Supplementary Material for: Low-dose aspirin and prevention of spontaneous preterm birth: is it worthwhile? A systematic review and meta-analysis

description: ABSTRACT
Introduction: Due to its anti-inflammatory properties, low-dose aspirin (LDA) has been theorized to mitigate spontaneous preterm birth (PTB). This systematic review and meta-analysis aims to report the available evidence to determine whether LDA administration prevents any spontaneous PTB. 
Methods: Embase, PubMed, Scopus, Cochrane Central Register, and Web of Science databases were searched, and the protocol was registered within the PROSPERO database (CRD42021288021). Data were examined using Review Manager 5.4 Software.
Results: The search yielded 261 relevant articles, from which 11 pertinent studies were included in this meta-analysis. While LDA showed a reduction of spontaneous PTB rates (Odds Ratio OR 0.93; 95% CI 0.84-1.04), the results lacked statistical significance. Nevertheless, LDA significantly reduced the rate of spontaneous PTB &lt; 34 weeks (6 studies; OR 0.85; 95% CI 0.78-0.93). Also, sub-group analysis of high-risk women (OR 0.96; 95% CI 0.78-1.17) and healthy women (OR 0.91; 95% CI 0.79-1.04) showed that LDA had a non-significant trend to reduce spontaneous PTB rates. Moreover, there was a statistically significant reduction in spontaneous PTB when LDA administration was initiated before 16 weeks (5 studies; OR 0.91; 95% CI 0.83-0.98), although not for LDA dosages below 100 mg (9 studies; OR 0.97; 95% CI 0.91-1.03).
Conclusion: This meta-analysis indicates a statistically significant reduction in spontaneous PTB rates associated with LDA administration only in spontaneous PTB pregnancies before 34 weeks (not any spontaneous PTB) and for LDA administration starting before 16 weeks. Although LDA can potentially prevent any spontaneous PTB, further well-conducted trials focusing specifically on this subgroup are warranted to provide robust evidence of its efficacy.
DOI: 10.6084/m9.figshare.29617874.v1
title: Supplementary Material for: Low-dose aspirin and prevention of spontaneous preterm birth: is it worthwhile? A systematic review and meta-analysis

description: ABSTRACT
Introduction: Due to its anti-inflammatory properties, low-dose aspirin (LDA) has been theorized to mitigate spontaneous preterm birth (PTB). This systematic review and meta-analysis aims to report the available evidence to determine whether LDA administration prevents any spontaneous PTB. 
Methods: Embase, PubMed, Scopus, Cochrane Central Register, and Web of Science databases were searched, and the protocol was registered within the PROSPERO database (CRD42021288021). Data were examined using Review Manager 5.4 Software.
Results: The search yielded 261 relevant articles, from which 11 pertinent studies were included in this meta-analysis. While LDA showed a reduction of spontaneous PTB rates (Odds Ratio OR 0.93; 95% CI 0.84-1.04), the results lacked statistical significance. Nevertheless, LDA significantly reduced the rate of spontaneous PTB &lt; 34 weeks (6 studies; OR 0.85; 95% CI 0.78-0.93). Also, sub-group analysis of high-risk women (OR 0.96; 95% CI 0.78-1.17) and healthy women (OR 0.91; 95% CI 0.79-1.04) showed that LDA had a non-significant trend to reduce spontaneous PTB rates. Moreover, there was a statistically significant reduction in spontaneous PTB when LDA administration was initiated before 16 weeks (5 studies; OR 0.91; 95% CI 0.83-0.98), although not for LDA dosages below 100 mg (9 studies; OR 0.97; 95% CI 0.91-1.03).
Conclusion: This meta-analysis indicates a statistically significant reduction in spontaneous PTB rates associated with LDA administration only in spontaneous PTB pregnancies before 34 weeks (not any spontaneous PTB) and for LDA administration starting before 16 weeks. Although LDA can potentially prevent any spontaneous PTB, further well-conducted trials focusing specifically on this subgroup are warranted to provide robust evidence of its efficacy.
DOI: 10.6084/m9.figshare.29617880
title: Supplementary Material for: Oral isotretinoin for facial discoid dermatosis (FDD): effectiveness, safety, and clinical suitability. Case report of complete response and literature review

description: Introduction: Facial discoid dermatosis (FDD) is a rare dermatological condition, first described in 2010. Its aetiology remains unknown and it is distinguished by its resistance to several treatments, both topical and systemic. The lack of documentation in the medical literature makes this disease a significant diagnostic and therapeutic challenge.
Case presentation: A 51-year-old woman consulted for the appearance of pruritic skin lesions on the face, neck and upper chest of 2 months' duration. She underwent various topical and systemic therapies without complete resolution over a period of 9 months. Histological examination revealed psoriasiform irregular epidermal hyperplasia, mild spongiosis, parakeratosis and hypogranulosis. In the superficial dermis, perivascular lymphocytic infiltrates were observed. Given the findings described and the refractoriness to multiple treatments employed, the diagnosis of FDD was established. Low-dose isotretinoin administration led to complete resolution of the skin lesions, with no associated adverse effects observed during the course of treatment. 
Conclusion: We present the first reported case of FDD in a middle-aged woman who was successfully treated with low-dose isotretinoin, resulting in complete resolution of the condition. This finding underlines the potential of isotretinoin as an effective and well-tolerated therapeutic option, especially in women of childbearing age. We also provide a comprehensive review of the treatments for FDD that have been documented in the literature.
DOI: 10.6084/m9.figshare.29617880.v1
title: Supplementary Material for: Oral isotretinoin for facial discoid dermatosis (FDD): effectiveness, safety, and clinical suitability. Case report of complete response and literature review

description: Introduction: Facial discoid dermatosis (FDD) is a rare dermatological condition, first described in 2010. Its aetiology remains unknown and it is distinguished by its resistance to several treatments, both topical and systemic. The lack of documentation in the medical literature makes this disease a significant diagnostic and therapeutic challenge.
Case presentation: A 51-year-old woman consulted for the appearance of pruritic skin lesions on the face, neck and upper chest of 2 months' duration. She underwent various topical and systemic therapies without complete resolution over a period of 9 months. Histological examination revealed psoriasiform irregular epidermal hyperplasia, mild spongiosis, parakeratosis and hypogranulosis. In the superficial dermis, perivascular lymphocytic infiltrates were observed. Given the findings described and the refractoriness to multiple treatments employed, the diagnosis of FDD was established. Low-dose isotretinoin administration led to complete resolution of the skin lesions, with no associated adverse effects observed during the course of treatment. 
Conclusion: We present the first reported case of FDD in a middle-aged woman who was successfully treated with low-dose isotretinoin, resulting in complete resolution of the condition. This finding underlines the potential of isotretinoin as an effective and well-tolerated therapeutic option, especially in women of childbearing age. We also provide a comprehensive review of the treatments for FDD that have been documented in the literature.
DOI: 10.6084/m9.figshare.29617883
title: Supplementary Material for: Cannabinoid Receptor Modulation in Focal Ischemic Stroke: A Systematic Review and Meta-Analysis of Infarct Volume and Behavioral Deficits in Animal Models

description: Introduction: Stroke is a leading cause of morbidity and mortality worldwide, with ischemic stroke accounting for the majority of cases. The endocannabinoid system (ECS), comprising cannabinoid receptors CB1 and CB2, has been implicated in regulating physiological processes and has shown potential neuroprotective effects in preclinical studies. However, the precise impact of cannabinoid receptor modulation on stroke outcomes remains unclear. This systematic review and meta-analysis aimed to evaluate the impact of cannabinoid receptor modulation on infarct volume and behavioral deficits in animal models of focal ischemic stroke, with a primary focus on infarct outcomes.
Method: A comprehensive literature search was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, yielding 29 eligible studies for inclusion. 
Results: The analysis revealed significant reductions in infarct volume with cannabinoid agonists, particularly CB1 and CB2 agonists, indicating their potential as neuroprotective agents. Subgroup analyses further highlighted specific agonists, such as ACEA and KN38-72717, as particularly effective in reducing infarct volume. Additionally, CB antagonists, particularly CB1 antagonists, such as SR141716, showed promising results in reducing infarct volume. Although improvements in neurological scores were observed with both agonists and antagonists, statistical significance was not reached, indicating the need for further investigation. 
Conclusion: These results highlight the potential of cannabinoid receptor modulation as a neuroprotective strategy in ischemic strokes and underscore the need for further research to elucidate the underlying mechanisms and optimize therapeutic approaches.
DOI: 10.6084/m9.figshare.29617883.v1
title: Supplementary Material for: Cannabinoid Receptor Modulation in Focal Ischemic Stroke: A Systematic Review and Meta-Analysis of Infarct Volume and Behavioral Deficits in Animal Models

description: Introduction: Stroke is a leading cause of morbidity and mortality worldwide, with ischemic stroke accounting for the majority of cases. The endocannabinoid system (ECS), comprising cannabinoid receptors CB1 and CB2, has been implicated in regulating physiological processes and has shown potential neuroprotective effects in preclinical studies. However, the precise impact of cannabinoid receptor modulation on stroke outcomes remains unclear. This systematic review and meta-analysis aimed to evaluate the impact of cannabinoid receptor modulation on infarct volume and behavioral deficits in animal models of focal ischemic stroke, with a primary focus on infarct outcomes.
Method: A comprehensive literature search was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, yielding 29 eligible studies for inclusion. 
Results: The analysis revealed significant reductions in infarct volume with cannabinoid agonists, particularly CB1 and CB2 agonists, indicating their potential as neuroprotective agents. Subgroup analyses further highlighted specific agonists, such as ACEA and KN38-72717, as particularly effective in reducing infarct volume. Additionally, CB antagonists, particularly CB1 antagonists, such as SR141716, showed promising results in reducing infarct volume. Although improvements in neurological scores were observed with both agonists and antagonists, statistical significance was not reached, indicating the need for further investigation. 
Conclusion: These results highlight the potential of cannabinoid receptor modulation as a neuroprotective strategy in ischemic strokes and underscore the need for further research to elucidate the underlying mechanisms and optimize therapeutic approaches.
DOI: 10.6084/m9.figshare.29617889
title: Supplementary Material for: Identification of Immune Candidate Genes in Post-Sepsis Syndrome: Linking Innate Immunity to Long-Term Autoimmune Responses

description: Introduction: Post-Sepsis Syndrome (PSS) is marked by persistent immune dysregulation, leading to long-term complications that overlap with autoimmune responses. Uncovering key immune-related candidate genes during PSS recovery can enhance our understanding of immune mechanisms involved in post-sepsis complications and inform targeted therapeutic strategies.
Methods: Analyze the GSE46955 dataset containing 24 peripheral blood mononuclear cell (PBMC) samples: 8 from the sepsis stage, 8 from the recovery phase, and 6 from healthy controls. Use the Linear Models for Microarray Data (limma) and Weighted Gene Co-expression Network Analysis (WGCNA) to identify differentially expressed genes (DEGs). Further explore key genes and pathways in sepsis recovery through protein–protein interaction (PPI) networks, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and a lipopolysaccharide (LPS)-induced mouse model.
Results: A total of 537 DEGs were identified, showing significant expression differences between sepsis and healthy controls. CD4, C1QA, and HLA-DRA were key hub genes in the PPI network, with increased expression in recovery samples, indicating roles in immune regulation. CD4 silencing worsened sepsis and reduced survival in mice, while CD4 overexpression improved outcomes.
Conclusion: Our findings highlight immune candidate genes that could serve as diagnostic and therapeutic targets in PSS, shedding light on the prolonged immune responses underlying sepsis recovery. These insights support the development of interventions targeting immune dysregulation in PSS, potentially applicable to other autoimmune conditions.
DOI: 10.6084/m9.figshare.29617889.v1
title: Supplementary Material for: Identification of Immune Candidate Genes in Post-Sepsis Syndrome: Linking Innate Immunity to Long-Term Autoimmune Responses

description: Introduction: Post-Sepsis Syndrome (PSS) is marked by persistent immune dysregulation, leading to long-term complications that overlap with autoimmune responses. Uncovering key immune-related candidate genes during PSS recovery can enhance our understanding of immune mechanisms involved in post-sepsis complications and inform targeted therapeutic strategies.
Methods: Analyze the GSE46955 dataset containing 24 peripheral blood mononuclear cell (PBMC) samples: 8 from the sepsis stage, 8 from the recovery phase, and 6 from healthy controls. Use the Linear Models for Microarray Data (limma) and Weighted Gene Co-expression Network Analysis (WGCNA) to identify differentially expressed genes (DEGs). Further explore key genes and pathways in sepsis recovery through protein–protein interaction (PPI) networks, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and a lipopolysaccharide (LPS)-induced mouse model.
Results: A total of 537 DEGs were identified, showing significant expression differences between sepsis and healthy controls. CD4, C1QA, and HLA-DRA were key hub genes in the PPI network, with increased expression in recovery samples, indicating roles in immune regulation. CD4 silencing worsened sepsis and reduced survival in mice, while CD4 overexpression improved outcomes.
Conclusion: Our findings highlight immune candidate genes that could serve as diagnostic and therapeutic targets in PSS, shedding light on the prolonged immune responses underlying sepsis recovery. These insights support the development of interventions targeting immune dysregulation in PSS, potentially applicable to other autoimmune conditions.
DOI: 10.6084/m9.figshare.29617898
title: Supplementary Material for: Neonatal Thymic Hemorrhage Secondary to Vitamin K Deficiency: A Case Report

description: Abstract
Introduction
 Vitamin K deficiency bleeding (VKDB) in newborns remains a preventable yet potentially devastating condition. Maternal malabsorption disorders such as Crohn’s disease may place infants at increased risk of coagulopathy due to impaired transplacental vitamin K transfer. This report describes a rare and severe case of VKDB in a preterm infant and his postpartum mother, highlighting the potential consequences of undiagnosed maternal vitamin K deficiency and emphasizing the importance of heightened awareness in high-risk pregnancies.
Case Report
 A 32-year-old woman with Crohn’s disease presented at 34+6 weeks’ gestation with preeclampsia and was delivered via urgent cesarean at 35+3 due to non-reassuring fetal testing. Her newborn son required resuscitation and developed severe coagulopathy with bruising, bleeding, and a large anterior mediastinal mass, later confirmed as hemorrhagic thymic tissue without neoplasm. Laboratory evaluation revealed markedly elevated INR and PIVKA-II, which improved with vitamin K and plasma transfusions. The mother experienced significant postpartum hemorrhage with a pelvic hematoma and coagulopathy, requiring transfusions, embolization, and vitamin K supplementation. Both mother and infant recovered following appropriate interventions. At 13 months, the infant had mild left-hand weakness but normal neurodevelopmental scores.
Conclusion
 This case highlights a rare but serious complication of maternal vitamin K deficiency resulting in neonatal coagulopathy and thymic hemorrhage. Given the increased risk in mothers with malabsorptive disorders such as Crohn’s disease, screening and supplementation protocols should be considered. Further studies are needed to guide optimal management of at-risk pregnancies to prevent VKDB in both mother and newborn.
DOI: 10.6084/m9.figshare.29617898.v1
title: Supplementary Material for: Neonatal Thymic Hemorrhage Secondary to Vitamin K Deficiency: A Case Report

description: Abstract
Introduction
 Vitamin K deficiency bleeding (VKDB) in newborns remains a preventable yet potentially devastating condition. Maternal malabsorption disorders such as Crohn’s disease may place infants at increased risk of coagulopathy due to impaired transplacental vitamin K transfer. This report describes a rare and severe case of VKDB in a preterm infant and his postpartum mother, highlighting the potential consequences of undiagnosed maternal vitamin K deficiency and emphasizing the importance of heightened awareness in high-risk pregnancies.
Case Report
 A 32-year-old woman with Crohn’s disease presented at 34+6 weeks’ gestation with preeclampsia and was delivered via urgent cesarean at 35+3 due to non-reassuring fetal testing. Her newborn son required resuscitation and developed severe coagulopathy with bruising, bleeding, and a large anterior mediastinal mass, later confirmed as hemorrhagic thymic tissue without neoplasm. Laboratory evaluation revealed markedly elevated INR and PIVKA-II, which improved with vitamin K and plasma transfusions. The mother experienced significant postpartum hemorrhage with a pelvic hematoma and coagulopathy, requiring transfusions, embolization, and vitamin K supplementation. Both mother and infant recovered following appropriate interventions. At 13 months, the infant had mild left-hand weakness but normal neurodevelopmental scores.
Conclusion
 This case highlights a rare but serious complication of maternal vitamin K deficiency resulting in neonatal coagulopathy and thymic hemorrhage. Given the increased risk in mothers with malabsorptive disorders such as Crohn’s disease, screening and supplementation protocols should be considered. Further studies are needed to guide optimal management of at-risk pregnancies to prevent VKDB in both mother and newborn.
DOI: 10.6084/m9.figshare.29617913
title: Supplementary Material for: Mitochondrial Glycosylation in Neuroinflammation Models

description: Introduction: Mitochondria are cellular energy factories, but their function declines with age in many tissues as well as disease pathophysiology. Mitochondrial proteins have sugar modifications called glycans, which regulate their function and localization. There is a knowledge gap on the impact of mitochondrial protein glycosylation on mitochondrial function and mediating neuroinflammation. We hypothesize that stimuli-specific neuroinflammatory treatments in microglia induce pathological changes in mitochondrial protein glycosylation and compromise mitochondrial function. 

Methods: The aim of this study was to establish a detailed microglial mitochondrial glycoprofile in different models of inflammation using lectins to identify the glycan-based markers of mitochondrial dysfunction. We use three different pathways of microglial activation: lipopolysaccharide (LPS), cytokines, and oxygen-glucose deprivation (OGD), revealing differences in mitochondrial glycosylation in different models of inflamed microglia. Mitochondrial lectin blots and lectin flow analysis were used to quantify the glycosylation changes due to different neuroinflammatory conditions. Seahorse Mito Stress assay was performed to assess mitochondrial function in each of these conditions.

Results: Lectin immunoblots of mitochondrial proteins and lectin flow studies with intact mitochondria were performed in three different neuroinflammation models using BV-2 microglial cells, revealing considerable stimuli-specific, differential mitochondrial glycosylation between these models and healthy controls. It was found that several glycans associated with mitochondria were differentially regulated during microglial activation. The observed changes in glycosylation trends were more drastic in OGD treatment as compared to other treatments, especially for complex and sialylated glycans. 

Conclusion: This study represents the first functional investigation of mitochondrial glycosylation in microglial inflammation models towards identifying glycosylation-based therapeutic targets.