cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20170700.v1
title: Table S1.docx

description: <strong>TABLE S1. </strong>Sequence information for all samples used in present study. “-” indicates missing data. Sequences downloaded from GenBank are marked in bold. Herbarium abbreviations are listed after the vouchers.
DOI: 10.6084/m9.figshare.20170712
title: Table S4.docx

description: <strong>TABLE S4. </strong>Nutlet morphology of <em>Isodon</em> species sampled in present study. Herbarium acronyms are listed in bracts after vouchers.
DOI: 10.6084/m9.figshare.20170712.v1
title: Table S4.docx

description: <strong>TABLE S4. </strong>Nutlet morphology of <em>Isodon</em> species sampled in present study. Herbarium acronyms are listed in bracts after vouchers.
DOI: 10.6084/m9.figshare.20170715
title: Table S2.docx

description: <strong>TABLE S2. </strong>Slope and <em>R</em><sup><em>2</em></sup> values of the linear regressions between patristic and uncorrected pairwise distances for the maximum likelihood tree from each locus.
DOI: 10.6084/m9.figshare.20170715.v1
title: Table S2.docx

description: <strong>TABLE S2. </strong>Slope and <em>R</em><sup><em>2</em></sup> values of the linear regressions between patristic and uncorrected pairwise distances for the maximum likelihood tree from each locus.
DOI: 10.6084/m9.figshare.20170718
title: Table S3.docx

description: <strong>TABLE S3. </strong>Features of plastomes of <em>Isodon </em>species.
DOI: 10.6084/m9.figshare.20170718.v1
title: Table S3.docx

description: <strong>TABLE S3. </strong>Features of plastomes of <em>Isodon </em>species.
DOI: 10.6084/m9.figshare.20102681
title: Defeat Recording Data

description: Main Behavior Pickle File: <br> Overall dictionary from (mouse,defeat_day)--&gt;data data.keys() # body_part_tracking, extracted_features, fiber_photometry <br> data['body_part_tracking] is a dictionary from body part to x,y coordinates over time (T frame, 120 Hz) <br> data['extracted_features'] is a 12xT matrix of feature traces (120 Hz) <br> data['fiber_photometry'] is a dictionary from 'fpts' or 'fpnac' to raw GCaMP recordings from the tail striatum or nucleus accumbens respectively (1017 Hz) <br> <br> Model Pickle Files: - 2 sets of random forest classifiers (trained RF model, cutoff for probability of behavior ocurring) dictionary of behavior name: (trained <code><strong>sklearn.ensemble</strong></code><strong>.RandomForestClassifier</strong>, classification probability cutoff) <br> - t-SNE model: dictionary: 'training': features in training set 'projection': <code><strong>sklearn.neural_network</strong></code><strong>.MLPClassifier </strong>maps raw features to t-SNE space 'labeled_map': where clusters are in t-SNE space <br> <br>
DOI: 10.6084/m9.figshare.20102681.v2
title: Defeat Recording Data

description: Main Behavior Pickle File: <br> Overall dictionary from (mouse,defeat_day)--&gt;data data.keys() # body_part_tracking, extracted_features, fiber_photometry <br> data['body_part_tracking] is a dictionary from body part to x,y coordinates over time (T frame, 120 Hz) <br> data['extracted_features'] is a 12xT matrix of feature traces (120 Hz) <br> data['fiber_photometry'] is a dictionary from 'fpts' or 'fpnac' to raw GCaMP recordings from the tail striatum or nucleus accumbens respectively (1017 Hz) <br> <br> Model Pickle Files: - 2 sets of random forest classifiers (trained RF model, cutoff for probability of behavior ocurring) dictionary of behavior name: (trained <code><strong>sklearn.ensemble</strong></code><strong>.RandomForestClassifier</strong>, classification probability cutoff) <br> - t-SNE model: dictionary: 'training': features in training set 'projection': <code><strong>sklearn.neural_network</strong></code><strong>.MLPClassifier </strong>maps raw features to t-SNE space 'labeled_map': where clusters are in t-SNE space <br> <br>
DOI: 10.6084/m9.figshare.16566474
title: Data and code for Bayesian analysis of changes in horizontal and vertical jump after different modes of resistance training. Accompanies the paper/manuscript Bayesian analysis of changes in standing horizontal and vertical jump after different modes of resistance training by Wilson et al (submitted to JSS 2022).

description: R files (R markdown & code) and data for paper detailing Bayesian analysis of jumper performance after 3 different resistance training protocols.
DOI: 10.6084/m9.figshare.16566474.v1
title: Data and code for Bayesian analysis of changes in horizontal and vertical jump after different modes of resistance training. Accompanies the paper/manuscript Bayesian analysis of changes in standing horizontal and vertical jump after different modes of resistance training by Wilson et al (submitted to JSS 2022).

description: R files (R markdown & code) and data for paper detailing Bayesian analysis of jumper performance after 3 different resistance training protocols.
DOI: 10.5281/zenodo.6528394
title: Training data for submitted paper "Wildfire Danger Prediction and Understanding with Deep Learning"

description: Training data for submitted paper "Wildfire Danger Prediction and Understanding with Deep Learning". To run the code in https://github.com/Orion-AI-Lab/wildfire_forecasting
description: Data for https://github.com/Orion-AI-Lab/wildfire_forecasting
DOI: 10.5281/zenodo.6528393
title: Training data for submitted paper "Wildfire Danger Prediction and Understanding with Deep Learning"

description: Training data for submitted paper "Wildfire Danger Prediction and Understanding with Deep Learning". To run the code in https://github.com/Orion-AI-Lab/wildfire_forecasting
description: Data for https://github.com/Orion-AI-Lab/wildfire_forecasting
DOI: 10.6084/m9.figshare.20097353
title: Factors affecting chronic low back pain among high school baseball players in Japan a pilot study

description: This study data included high school baseball players in Fukui Prefecture, Japan, which was used in the study on Nakao ,Imai, Hamada (2022).
DOI: 10.6084/m9.figshare.20097353.v2
title: Factors affecting chronic low back pain among high school baseball players in Japan a pilot study

description: This study data included high school baseball players in Fukui Prefecture, Japan, which was used in the study on Nakao ,Imai, Hamada (2022).
DOI: 10.6084/m9.figshare.20097353.v1
title: Factors affecting chronic low back pain among high school baseball players in Japan a pilot study

description: This study data included high school baseball players in Fukui Prefecture, Japan, which was used in the study on Nakao ,Imai, Hamada (2022).
DOI: 10.5281/zenodo.6593174
title: Prioritization of semantic over visuo- perceptual aspects in multi-item working memory

description: All data and code supporting Prioritization of semantic over visuo- perceptual aspects in multi-item working memory
DOI: 10.5281/zenodo.6593173
title: Prioritization of semantic over visuo- perceptual aspects in multi-item working memory

description: All data and code supporting Prioritization of semantic over visuo- perceptual aspects in multi-item working memory
DOI: 10.5281/zenodo.6772234
title: Supplementary Information for "G-type Halohydrin Dehalogenases Catalyze Ring Opening Reactions of Cyclic Epoxides with Diverse Anionic Nucleophiles"

description: This is the external Supplementary Information for our publication "G-type Halohydrin Dehalogenases Catalyze Ring Opening Reactions of Cyclic Epoxides with Diverse Anionic Nucleophiles". <strong>IMPORTANT: This is only the zero version. To access the full dataset, see the most recent version</strong>(s) (either via the banner above or the version navigator to the right). The .pdf file contains the Supplementary Information: author contributions, experimental procedures, additional discussions and supplementary items, among others. The .zip file contains the raw NMR data for all compounds described in the manuscript.
DOI: 10.22008/fk2/q81rjv/dh0vdj
title: SCO_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/7puerz
title: NUK_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/xqnbkf
title: NUK_K_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/twayqw
title: TAS_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/sicxin
title: KAN_B_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/v79rps
title: UPE_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/urod3r
title: KPC_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/wzq1gz
title: KAN_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/bvhjca
title: UPE_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/5lvr1a
title: KPC_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/pexhcn
title: NUK_K_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/oohfp6
title: UPE_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/a0w98u
title: NUK_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/ezfrdm
title: NUK_N_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/gjkvio
title: NUK_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/4by8py
title: MIT_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/wgcaux
title: KAN_M_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/sic6ee
title: QAS_A_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/oxdwgi
title: NUK_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/v0arrp
title: QAS_M_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/mf3m5q
title: KAN_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/jxbjyt
title: THU_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/ricxw0
title: QAS_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/vcvu4u
title: UPE_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/3ltelw
title: EGP_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/n6nemn
title: KAN_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/cuce37
title: KPC_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/56hzak
title: readme PROMICE AWS data.pdf

description: :unav
DOI: 10.22008/fk2/q81rjv/cbenei
title: SCO_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/agmmeu
title: QAS_M_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/w6awx3
title: KAN_M_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/cqpgcy
title: KPC_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/meuhsw
title: THU_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/vsgwpp
title: TAS_A_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/r52slt
title: THU_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/hignkk
title: SCO_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/jy9alu
title: TAS_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/dk3haa
title: QAS_A_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/w7zpp1
title: TAS_A_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/yqevqw
title: THU_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/9y0ye8
title: SCO_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/t7eljz
title: QAS_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/zqxb8k
title: TAS_A_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/ljwsgl
title: QAS_A_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/2amu4l
title: KPC_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/2ashr9
title: KAN_M_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/xv3lsk
title: EGP_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/d6ow6q
title: EGP_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/fmeyrt
title: UPE_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/cvthyh
title: NUK_N_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/awa5ul
title: KAN_B_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/e9iw1k
title: SCO_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/phjfem
title: QAS_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/8os92q
title: TAS_U_month_v02.txt

description: :unav
DOI: 10.6084/m9.figshare.20170826
title: data

description: Zircon U-Pb ages of granitic rocks from the Diancang Shan complex, Ar-Ar geochronology of rocks from the southern Chong Shan complex.
DOI: 10.6084/m9.figshare.20170826.v1
title: data

description: Zircon U-Pb ages of granitic rocks from the Diancang Shan complex, Ar-Ar geochronology of rocks from the southern Chong Shan complex.
DOI: 10.22008/fk2/q81rjv/sybchi
title: TAS_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/vo9uuc
title: QAS_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/ubxofl
title: SCO_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/noffaq
title: KAN_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/t7sxas
title: QAS_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/o6hvz8
title: TAS_U_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/lfqwyw
title: NUK_N_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/twjyif
title: TAS_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/em6hao
title: MIT_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/746vjo
title: NUK_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/icvz7v
title: QAS_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/hzkhwy
title: THU_U_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/nxbdjx
title: THU_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/kblmyl
title: NUK_K_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/uvagzz
title: KPC_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/yvyiq5
title: KAN_L_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/sa6xvk
title: KAN_B_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/v4jnj7
title: KAN_U_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/pfrpvm
title: NUK_L_month_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/9sca9t
title: UPE_L_day_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/len3qv
title: QAS_M_hour_v02.txt

description: :unav
DOI: 10.22008/fk2/q81rjv/1gpul5
title: MIT_month_v02.txt

description: :unav
DOI: 10.22002/nkmbg-kmr58
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-08-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Take the sedimented cell clumps.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2015-08-10-28_slicer16531.jpg: periodic structure ycw2015-08-10-28_slicer16530.jpg: TCP basal body
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-28/3dimage_73145/</p>20150810_AK20_20028_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 14.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-28/3dimage_73145/20150810_AK20_20028_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150810_AK20_20028.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-28/rawdata/20150810_AK20_20028.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jeq1r-k2653
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-08-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Take the sedimented cell clumps.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-33/3dimage_73150/</p>20150810_AK20_20033_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 14.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-33/3dimage_73150/20150810_AK20_20033_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150810_AK20_20033.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-33/rawdata/20150810_AK20_20033.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y9zfp-4j865
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-08-15</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-45/rawdata/</p>Cb_20170816_Cb1pH4d50044.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-45/rawdata/Cb_20170816_Cb1pH4d50044.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_20170816_Cb1pH4d50044_part61_3.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-45/3dimage_88034/Cb_20170816_Cb1pH4d50044_part61_3.rec"
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DOI: 10.22002/sn3z4-j6y79
title: LCV_Legionella pneumophila

description: THP1 cells were infected with Legionella wild type cells and thereafter phagosomes (LCV) were purified by sucrose density gradient method. Phagoseomes were fixed before shipment.
description: <strong>Tilt Series Date:</strong> 2017-04-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Phagosome isolation method as described by Joelle Gabay (Endnote).</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-36/rawdata/</p>LCV_wt_20170413abc0001.mrc,  Tilt Series (Pixel Size 0.39 nm), 911.4 MB  
        
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        LCV_wt_20170413abc0001_part32_6.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
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