cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j02j69tb
title: CIL:36731, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: In more extreme cases microfilamentous material builds up into a thick layer surrounding the DV-I membrane as well as the fusion sites between the acidosomes and the DV-I. A similar thick meshwork was not observed between lysosomes and the DV-II. TEM taken on 5/3/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,000X. Bar = 0.5µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0j38rh9
title: CIL:36730, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Higher magnification of a QF-DE image of an acidosome with a large inward indentation of its membrane. The fractured P-face of the indentation shows many large IMPs. The acidosome lumen is honeycombed where the vitreous ice was etched away. TEM taken on 5/18/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 40,500X. Bar = 0.1µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0rj4hdw
title: CIL:36765, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Stacks of 4 or 5 kinetodesmal fibers occupy the longitudinal ridges in a cross-sectioned cell. The kds lie along the right side of each ridge (cell‚Äôs left side), and as the kds are tapered, the smallest cross sections lie closest to the ridge tip and these sections are also closest to the distal tips of the kds. Such a kd arose from basal bodies 4 or 5 cortical units posterior to the plane of the cross-section. Lateral ridges do not contain kds. TEM taken on 12/28/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 17,500X. Bar = 0.25µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38901) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0sn07wp
title: CIL:36764, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Kinetodesmal fibers arise from the proximal ends of the basal bodies and curve upward and to the cell‚Äôs right of the kinety. Together with kds arising from more posterior basal bodies they form a stack of overlapping fibers in the longitudinal ridge. A ribbon of microtubules also arises from the proximal end of the basal body and curves posteriorly passing to the right of the kinety. The plasma membrane and outer alveolar membrane were fragmented in this cell. TEM taken on 11/2/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.2µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38900) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j008648q
title: CIL:36762, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38898) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Cross section of a cilium showing the 9 + 2 axoneme. Two dynein arms, inner and outer, of heterogeneous appearance arise from the complete microtubule of each doublet. Spokes extend from the doublets toward the central pair. Other densities extend from the central pair and between some of the outer doublets and the membrane. A heavy glycocalyx (polysaccharide coat) is found on the outside of the ciliary membrane as well as the plasma membrane. TEM taken on 2/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 47,100X. Bar = 0.1µm.
DOI: 10.6075/j0tt4pv5
title: CIL:36752, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Following digestion, that only occurs in the phagolysosome (DV-III), the membrane of these DVs undergoes extensive tubulation. These tubules are ~45nm in diameter and arise from the cytosolic surface of the DV-III in clumps of 3 to 6 tubules per clump. The tubules contain a glycocalyx-like lining in their lumens which leaves only a 10nm diameter opening in the center of the tubule (see Allen and Fok, J. Cell Biol. 99:1955-1959, 1984). TEM taken on 4/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µm.
DOI: 10.6075/j0h70dqw
title: CIL:36740, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: Secondary lysosomes in quick-freeze deep-etch images lie against the smooth E-face of a phagoacidosome (DV-II) membrane. The P-fracture face of one fairly large lysosome lies close to the DV-II but no fusion pore is seen. The P-face of the lysosome has a moderate number of IMPs, more IMPs than on its E-fracture face. TEM taken on 5/25/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0ns0stj
title: CIL:36737, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: Secondary lysosomes contain a lumen in quick-freeze deep-etch (QF-DE) images resembling etched matrix proteins rather than the honeycomb appearance of acidosomes. Lysosomes often have one or more paracrystalline layers in their lumens. The brush of filaments lining the insides of lysosomal membranes seen in thin sections is not particularly evident in these QF-DE views of fractured lysosomes. TEM taken on 5/20/95 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0zw1jv9
title: CIL:36735, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Cytochalasin B blocks DV release from cytopharynx. In this micrograph a very large nascent vacuole has been retained at the cytopharynx of this cell that had been exposed to cytochalasin B. Many acidosomes are docked at the membrane of this vacuole. Some acidosomes contain HRP reaction product and some are free of HRP. The cell had been exposed to HRP and cytochalasin B for 10 minutes during which no new DV-I had been released. During this exposure to CB, some discoidal vesicles continue to move to the cytopharynx and fuse, acidosomes continue to move to the nascent phagosome and dock, and endosome and carrier vesicle formation continues and they continue to fuse with acidosomes. CB has done two things, it has prevented normal phagosome release from the cytopharynx and it has also inhibited acidosome fusion with the phagosomes (Allen and Fok, J. Cell Biol. 97:566-570, 1983) once they form. For this study cells were exposed to 0.3mM of CB for 10-20 min. The lack of acidosome fusion with the few phagosomes that do pinch off during CB exposure was supported by the fact that the phagosomes became only mildly acid. TEM taken on 8/29/84 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 1,610X. Bar = 2µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j00863z8
title: CIL:36745, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The entire membrane of the phagolysosome does appear to have been changed into that similar to the lysosome membrane which bears a thick glycocalyx (polysaccharide coat) and no longer appears to be like the phagoacidosome which does not have a thick glycocalyx. As no patches of acidosome-like membrane remain it can be assumed that the acidosome-like membrane has been removed. Fibrous material lines the cytosolic side of the early DV-III similar to that found at docking sites between the lysosomes and the DV-II. TEM taken on 3/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. Bar = 0.2µm.
DOI: 10.6075/j0bk1b8h
title: CIL:36744, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: As with acidosome fusion with the DV-I, lysosome fusion with the DV-II also seems to occur nearly all at once resulting in a phagolysosome (DV-III) with a highly irregular surface. However, unlike the transformation of DV-I into DV-II where the DV-I membrane is removed by immunogold-labeled tubules we have very little immunological evidence that the DV-II membrane is removed during the transition from phagoacidosome to phagolysosome. Although there is some morphological indication for tubular and vesicular formation around these early phagolysosomes that might indicate a similar transformation. The vacuole contents seen here are thought to be the lipid micelles of the axenic medium. TEM taken on 2/27/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 7,000X. Bar = 1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0ft8jzz
title: CIL:36761, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38897) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: A high magnification view of the above septum in planar view and the septal pores that connect adjacent alveoli. The pores always lie next to the junction of the septum with the outer alveolar membrane. TEM taken on 11/2/68 by R. Allen with Philips 300 operating at 60kV. Neg. 39,900X. Bar = 0.1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0765d9k
title: CIL:36742, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Acid phosphatase is synthesized in the biosynthetic pathway in the ER and packaged in the TGN into primary lysosomes which are passed on to secondary lysosomes. Primary lysosomes are small vesicles of 75-100nm filled with acid phosphatase and no substrate. They probably fuse with secondary lysosomes before the secondary lysosomes fuse with the DV-II. Small vesicles of the size of primary lysosomes are near secondary lysosomes. TEM taken on 3/26/79 by J. Muraoka with Hitachi HU11A operating at 75kV. Neg. 25,500X. Bar = 0.2µm.
description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Glutaraldehyde fixation followed by Gomori Staining, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j03777c8
title: CIL:36753, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40565) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The tubules arising from late DV-III expand at their distal ends into rounded shapes. These expanded ends contain a glycocalyx like the secondary lysosomes and large IMPs also like secondary lysosomes. TEM taken on 8/7/80 by R. Allen with Hitachi HU11A TEM. Neg. 12,250X. Bar = 0.5µm. Part published in J. Cell Biol. 99:1955-1959, 1984. Adapted with permission.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed