DOI: 10.22002/mt372-7x280
title: Bdellovibrio attack-phase

description: Bdellovibrio in the mixture with Vibrio cholerae MKW1383.Imaged by Janelia farm Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2013-05-01-10_slicer4860.jpg: Pillus .  Intermembrane vesicle .  Nucleoid ycw2013-05-01-10_slicer5017.jpg: Nucleoid ycw2013-05-01-10_slicer5019.jpg: Unknown intermembrane ring ycw2013-05-01-10_slicer5021.jpg: Flagellar motor ycw2013-05-01-10_slicer5022.jpg: Unknown ycw2013-05-01-10_slicer5025.jpg: Protein-decorated intermembrane granule ycw2013-05-01-10_slicer5029.jpg: Unknown filament ycw2013-05-01-10_slicer5031.jpg: Unknown filament ycw2013-05-01-10_slicer5033.jpg: Chemoreceptor ycw2013-05-01-10_slicer5035.jpg: Pilli ycw2013-05-01-10_slicer5405.jpg: Nucleoid ycw2013-05-01-10_slicer5406.jpg: Pilli ycw2013-05-01-10_slicer9414.jpg: periplasmic vesicles .  Bdellovibrio in the mixture with Vibrio cholerae .  snapshot by Megan ycw2013-05-01-10_slicer9416.jpg: tube end-on view, 8.7 nm diameter .  Bdellovibrio attack phase .  snapshot by Megan ycw2013-05-01-10_slicer9417.jpg: tube end-on view, 8.5 nm diameter .  Bdellovibrio attack phase .  snapshot by Megan ycw2013-05-01-10_slicer9418.jpg: tube long view, about 9 nm diameter .  Bdellovibrio attack phase .  snapshot by Megan ycw2013-05-01-10_slicer9419.jpg: tube end on view, Bdellovibrio attack phase .  about 9nm diameter .  snapshot by Megan
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-10/rawdata/</p>grid3-tiltseries0010.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        grid3-tiltseries0010_part131_2.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
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        grid3-tiltseries0010_mode1_1k_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 258.4 MB  
        
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