DOI: 10.5061/dryad.10qj1
title: Data from: Cryptic species in the mountaintops: species delimitation and taxonomy of the Bembidion breve species group (Coleoptera: Carabidae) aided by genomic architecture of a century-old type specimen

description: The breve species group includes closely related Bembidion Latreille
 ground beetles commonly found at high elevation in the mountains of
 western North America. For several decades, the group has been considered
 to consist of two species. Here, we present evidence from morphological,
 molecular and geographic data that the group contains nine species:
 Bembidion ampliatum, B. breve, B. geopearlis, B. laxatum, B. lividulum, B.
 oromaia, B. saturatum, B. testatum and B. vulcanix. We describe three
 species (B. geopearlis, B. oromaia and B. vulcanix) as new and resurrect
 four previously synonymized names (B. ampliatum, B. lividulum, B.
 saturatum and B. testatum). Species diversity is highest throughout the
 Cascades in Oregon and Washington, and Sierra Nevada of California, where
 up to seven species can occur in sympatry. We resolved challenging
 nomenclatural issues through analysis of sequences obtained from
 century-old type specimens by using a novel application of rDNA copy
 number analysis – an approach that may prove useful for other historical
 specimens.
description: breve.group.revision.10The Mesquite file used to infer gene trees for the five genes analyzed in the study.Beauti.breve.STACEY.6.500MInput file used in STACEY species delimitation analysis as implemented in BEAST (file in readable by BEAUTI under the STACEY template option).Bembidion_aeruginosum_DNA3890_rDNAReference sequence of the ribosomal DNA cistron taken from a de novo assembly of the sister taxon to the breve group, Bembidion aeruginosum. This reference sequence was used in the read mapping analyses to produce copy number profiles of the rDNA.ReadMapping.LIB0208.B.laxatum.DNA4918A SAM file with the results of mapping shotgun sequencing reads (available on SRA) from Bembidion laxatum (Maddison voucher DNA4918) to a reference sequence (included in this submission) of B. aeruginosum.ReadMapping.LIB0248.B.laxatum.DNA4149A SAM file with the results of mapping shotgun sequencing reads (available on SRA) from Bembidion laxatum (Maddison voucher DNA4149) to a reference sequence (included in this submission) of B. aeruginosum.ReadMapping.LIB0249.B.ampliatum.DNA4245A SAM file with the results of mapping shotgun sequencing reads (available on SRA) from Bembidion ampliatum (Maddison voucher DNA4245) to a reference sequence (included in this submission) of B. aeruginosum.ReadMapping.LIB0250.B.ampliatum.DNA3593A SAM file with the results of mapping shotgun sequencing reads (available on SRA) from Bembidion ampliatum (Maddison voucher DNA3593) to a reference sequence (included in this submission) of B. aeruginosum.ReadMapping.LIB0245.B.lividulum.DNA4165A BAM file with the results of mapping shotgun sequencing reads (available on SRA) from Bembidion lividulum (Maddison voucher DNA4165) to a reference sequence (included in this submission) of B. aeruginosum.ReadMapping.LIB0243.B.lividulum.DNA4165.BAMReadMapping.LIB0245.B.lividulum.DNA5032A BAM file with the results of mapping shotgun sequencing reads (available on SRA) from Bembidion lividulum (Maddison voucher DNA5032) to a reference sequence (included in this submission) of B. aeruginosum.ReadMapping.LIB0182.B.lividulum.Lectotype.DNA4889A SAM file with the results of mapping shotgun sequencing reads (available on SRA) from the electrotype of Bembidion lividulum Casey 1918 (Maddison voucher DNA4889) to a reference sequence (included in this submission) of B. aeruginosum.