DOI: 10.5061/dryad.ns8kp8j
title: Data from: Molecular and Morphological Variation among Populations of Pediomelum tenuiflorum (Pursh) A.N. Egan (Fabaceae) in Nebraska, USA

description: Individuals of Pediomelum tenuiflorum, “wild alfalfa”, from disjunct
 populations in Nebraska vary extensively in their overall gestalt. Those
 in the western and central part of the state have a very slender growth
 habit, with thin stems and few, small flowers; whereas, those in the
 southeast have a very robust growth habit with heavy-looking stems and
 many tightly clustered flowers. For nearly 200 years, taxonomists have
 alternated between splitting P. tenuiflorum into two species, with the
 many-flowered morphotype named P. floribundum, and lumping all the
 morphological variants into one species as they are now. In this study, we
 investigated morphological and molecular characters that could be used to
 clarify taxonomic classifications of these morphotypes. We measured 10
 morphological characters on 51 specimens and sequenced nearly 300,000
 nucleotide characters on the Illumina platform from three cellular genomes
 in seven samples of Pediomelum plus an outgroup taxon. Results revealed
 six significantly different morphological characters but ambiguous
 evolutionary histories of the plastid and mitochondrial genomes in P.
 tenuiflorum. Our complete plastid genomes and genes and noncoding regions
 of the mitochondrial genome may be used as a foundation for studying the
 evolutionary histories of these genomes. Additionally, we identified seven
 highly variable genomic regions in the chloroplast genome upon which a
 molecular phylogenetic investigation on an expanded set of samples from
 across the species’ geographic distribution can be conducted to further
 define the taxonomic placements of P. tenuiflorum and P. floribundum.
description: Aln-Ped-cpDNAAlignment of complete plastid genomes for eight samples in Fabaceeae (Ladeania lanceolata, Pediomelum argophylum, (2) Pediomelum digitatum, Pediomelum esculentum, (3) Pediomelum tenuiflorum)Aln-Ped-nrDNAAlignment of nrDNA (ITS1, 5.8S, and ITS2) for eight samples in Fabaceeae (Ladeania lanceolata, Pediomelum argophylum, (2) Pediomelum digitatum, Pediomelum esculentum, (3) Pediomelum tenuiflorum)Aln-Ped-mtDNAAlignment of 38 regions of the mitochondrial genome for eight samples in Fabaceeae (Ladeania lanceolata, Pediomelum argophylum, (2) Pediomelum digitatum, Pediomelum esculentum, (3) Pediomelum tenuiflorum)Aln-Ped-8taxa-3genomes-FINALAlignment of complete plastid genomes, nrDNA (ITS1, 5.8S, and ITS2), and 38 regions of the mitochondrial genome for eight samples in Fabaceeae (Ladeania lanceolata, Pediomelum argophylum, (2) Pediomelum digitatum, Pediomelum esculentum, (3) Pediomelum tenuiflorum)Aln-Pediomelum_32taxa-6regionsAlignment of a concatenation the following genomic regions: nrDNA ITS1, 5.8S, and ITS2 and five plastid regions (matK, rpoB-trnC, trnD-trnY, trnL-F, and trnS-trnG). trnL-F includes a portion of the trnL intron, trnL-UAA exon 2, and the trnL-trnF intergenic spacer (IGS). Alignment includes 32 samples as follows: Ladeania juncea, (2) Ladeania lanceolata, Orbexilum melanocarpum, Orbexilum virgatum, (2) Pediomelum argophyllum, Pediomelum aromaticum, Pediomelum californicum, Pediomelum canescens, Pediomelum castoreum, Pediomelum cuspidatum, Pediomelum cyphocalyx, (2) Pediomelum digitatum, (2) Pediomelum esculentum, Pediomelum humile, Pediomelum hypogaeum, Pediomelum latestipulatum, Pediomelum linearifolium, Pediomelum megalanthum, Pediomelum pariense, Pediomelum pentaphyllum, Pediomelum piedmontanum, Pediomelum reverchonii, (4) Pediomelum tenuiflorum, Rupertia hallii, Rupertia physodes