cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/t5419-gbr46
title: Pva 121509 MP43

description: Prosthecobacter vanneervenii, 10feb07a_MP43_Pva_stat54h_003,
description: <strong>Tilt Series Date:</strong> 2010-02-07</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Prosthecobacter vanneervenii</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
  mpa2010-02-07-20_slicer6024.jpg: Periplasmic vesicle mpa2010-02-07-20_slicer6026.jpg: Tube? mpa2010-02-07-20_slicer6029.jpg: Filament mpa2010-02-07-20_slicer6032.jpg: Filament mpa2010-02-07-20_slicer10156.jpg: periplasmic vesicle .  snapshot by Megan .  Prosthecobacter vanneervenii
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-02-07-20/rawdata/</p>10feb07a_MP43_Pva_stat54h_003.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        10feb07a_MP43_Pva_stat54h_003_full.rec,  Reconstruction (Pixel Size 1.9 nm), 838.9 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.vaetat
title: Occurrence Download

description: A dataset containing 525 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Symphyotrichum novae-angliae",
 "Country is Switzerland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 525 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048405-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/xwmyk-k9e46
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-09-30</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-68.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-73/rawdata/</p>RCC745_G1tovis_228.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-73/rawdata/RCC745_G1tovis_228.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_228.rec,  Reconstruction (Pixel Size 1.26 nm), 273.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-73/3dimage_12233/RCC745_G1tovis_228.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/54t8g-hn241
title: CCcenK_glu

description: C. crescentus CenK, glucose depletion .
description: <strong>Tilt Series Date:</strong> 2006-09-24</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.7°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 36 hours in PYE + 0.1% glucose</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-09-24-1/rawdata/</p>CCcenR_glu14.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-09-24-1/rawdata/CCcenR_glu14.mrc"
        > <i class="download icon"></i> Download </a></p>

        CCcenR_glu14.rec,  Reconstruction (Pixel Size 1.34 nm), 349.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-09-24-1/3dimage_3000/CCcenR_glu14.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5g363-d9j92
title: CCcenR_glu

description: C. crescentus CenR, glucose depletion .
description: <strong>Tilt Series Date:</strong> 2006-07-06</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 56.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-9/rawdata/</p>CCcenR_glu04.mrc,  Tilt Series (Pixel Size 1.2 nm), 956.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-9/rawdata/CCcenR_glu04.mrc"
        > <i class="download icon"></i> Download </a></p>

        CCcenR_glu04.rec,  Reconstruction (Pixel Size 2.4 nm), 267.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-9/3dimage_2960/CCcenR_glu04.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ww8cpc
title: Occurrence Download

description: A dataset containing 2263 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Albizia kalkora"
 ]
}

 The dataset includes 2263 records from 83 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048404-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/scx5n-34x93
title: E.coli   "strain 4" I241E

description: E.coli chemotaxis mutant  "strain 4" I241EReceptors (tsr on chromosome) locked in a CheA activating state
description: <strong>Tilt Series Date:</strong> 2012-03-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain generated by John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown in TB (gentamycin) overnight at 30 degrees. Diluted in fresh buffer and grown to log phase. Then incubated with penicillin for one hour.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: -4 offset, 3.5 s blot time, no drain, 100 percent humidity</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-07-17/rawdata/</p>strain4marchsevenparttwo0003.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-07-17/rawdata/strain4marchsevenparttwo0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain4marchsevenparttwo0003_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-07-17/3dimage_17546/strain4marchsevenparttwo0003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.zdg9sq
title: Occurrence Download

description: A dataset containing 16500 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-109.5594 33.77787,-97.2912 34.35572,-98.31355 47.42402,-103.82535 48.84641,-110.11284 46.00808,-112.18194 44.49032,-115.1601 40.00087,-112.71535 35.46697,-109.5594 33.77787))",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Bison bison"
 ]
}

 The dataset includes 16500 records from 38 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048406-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/4yd9x-p2321
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-53/rawdata/</p>20141022_YWC121_40023.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-53/rawdata/20141022_YWC121_40023.mrc"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_40023_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-53/3dimage_47497/20141022_YWC121_40023_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7dhx5-6wd43
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC106
description: <strong>Tilt Series Date:</strong> 2014-07-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-2/rawdata/</p>20140707_YWC106_10002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-2/rawdata/20140707_YWC106_10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140707_YWC106_10002_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-2/3dimage_44381/20140707_YWC106_10002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4bqtb-f6f40
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-82/rawdata/</p>20131221_YWC063_20039.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-82/rawdata/20131221_YWC063_20039.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_20039_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-82/3dimage_36504/20131221_YWC063_20039_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7jv7z-ty343
title: Rhodobacter sphaeroides JPA 1558

description: cryosections of R sphaeroides strain JPA1558 (TlpT-YFP)
description: <strong>Tilt Series Date:</strong> 2013-06-03</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grow  (1 day) in SUX medium + Naldixic acid (6.25 µl of 20mg/ml stock) + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*Spin down 5ml per strain, resuspend pellets (barely visible) in SUX medium + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*grow overnight.</p>
<strong>Sample Preparation:</strong> cryosectioning by MArk LAdinsky</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-45/rawdata/</p>atlas_grid-2_7x7cryosections0018.mrc,  Tilt Series (Pixel Size 0.38 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-45/rawdata/atlas_grid-2_7x7cryosections0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_grid-2_7x7cryosections0018_full.rec,  Reconstruction (Pixel Size 0.76 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-45/3dimage_27279/atlas_grid-2_7x7cryosections0018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2h5we-zs556
title: Hippocampal Neurons (cultured)

description: Plunge frozen at DIV 14
description: <strong>Tilt Series Date:</strong> 2014-08-21</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-4/rawdata/</p>082014_neurons0007.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-4/rawdata/082014_neurons0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        082014_neurons0007_full.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-4/3dimage_45571/082014_neurons0007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/03s4g-pr792
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-12/3dimage_11635/</p>RCC745_MGtovis_324.rec,  Reconstruction (Pixel Size 1.26 nm), 266.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-12/3dimage_11635/RCC745_MGtovis_324.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_324.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-12/rawdata/RCC745_MGtovis_324.mrc"
        > <i class="download icon"></i> Download </a></p>