cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0639ngw
title: CIL:11951, Chrysochraon japonicas, spermatocyte. In Cell Image Library

description: The time-lapse movie made in 1974 shows meiosis in spermatocytes of Chrysochraon japonicas (grasshopper) as seen with the polarizing microscope. The principal birefringent components are the spindle microtubules. Their dynamic assembly and disassembly creates the flickering 'Northern Lights' effect.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
DOI: 10.6075/j0jm28z7
title: CIL:40304, Petunia, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Petunia pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j0mw2gvr
title: CIL: 53985. In Cell Image Library

description: STE family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Visualization methods: confocal microscopy
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
DOI: 10.6075/j0j1029m
title: CIL:10706, Notophthalmus viridescens, oocyte. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Oocytes were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 5 KX.
description: High voltage TEM image of meiotic newt oocyte nuclei showing transcription on looped lampbrush chromosomes. This micrograph, was recorded at a tilt angle of 40 degrees. The image is grouped with one of the same sample area recorded at a 50 degree tilt. A stereo examination of the pair provides an oblique 3D view.
DOI: 10.6075/j07m06kx
title: CIL:35411, Danio rerio, neurecto-epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: bodipy ceramide
Processing history: unprocessed raw data
description: The interstitial space of the embryo has been labeled with the fluorescent lipid Bodipy-ceramide; anterior left.
description: Zebrafish Notogenesis and Early Neurulation. Mediolateral intercalation produces convergent-extension of the notochordal rudiment. Cells within the center of the notochord elongate and are incorporated into the notochordal-somitic boundary. The medial thickening of the neural plate becomes visible along the embryonic midline. As the neural plate converges toward the embryonic midline, the notochord becomes depressed ventrally. Oriented neuroepithelial cell divisions first take place in the ventral medial thickening (i.e. presumptive floorplate) near the neural midline after mediolateral intercalation is well-advanced in the notochordal rudiment. The otic placode comes visible as the embryo undergoes a torsional rotation.
DOI: 10.6075/j0qf8rtb
title: CIL:34616, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Most wild type Tetrahymena have a macronucleus and a micronucleus. The micronucleus, seen here, is essentially nonfunctional during interphase. Its function is to replicate and pass a complete copy of the genome to each daughter cell and to give rise to a new macronucleus following sexual reproduction. Many laboratory strains, unlike strain W which is shown here, have lost their micronucleus and seem to be able to live indefinitely without a micronucleus, however, they can no longer undergo meiosis and sexual reproduction. TEM taken on 4/8/65 by R. Allen with RCA EMU3F operating at 50kV. Neg. 9,100X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34534) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0319tfw
title: CIL:48816, Mus musculus, MC57-Cerulean. In Cell Image Library

description: To determine the origin (local vs. circulating) of tumor associated fibroblasts, a parabiotic pair of mice was created where only one mouse expressed EGFP under the control of the type I collagen alpha-1 promoter (COL-EGFP), and the other mouse expressed DsRed ubiquitously. On each mouse of the parabiotic pair, MC38-Cerulean cancer cells were injected on the distal flank. This folder contains images from the tumor grown in the COL-EGFP mouse, freshly explanted and analyzed ex vivo. The blue channel shows cancer cells, the red channel shows DsRed cells, the green channel shows COL-EGFP+ fibroblasts. Image name: 120513 Parabiot Rag MC57 COLEGFP tumor flank.lif - 6 

Project: 20389
description: Item type: confocal microscopy
description: Mice were euthanized and tumors were freshly explanted. 1-2 mm thick sections were cut at different depths and kept in PBS during the imaging session. Fluorescent confocal microscopy was done using a Leica SP5 II TCS tandem scanner 2-photon spectral confocal microscope with XY motorized stage, using a 25X/NA 0.95 water lens from Leica.
DOI: 10.6075/j0fx78s2
title: CIL:39228, Psophus stridulus, spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: film
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: The movie was originally recorded on 35mm film, and later transferred to CD format. The text at the beginning of the movie translates approximately as follows:

Meiosis division II, metaphase (ca. 4-5 hr) and anaphase (ca. 1 hr).

See also:
Michel, K. (1941): Die Darstellung von Chromosomen mittels des Phasenkontrturwissenschaften 29, 61-62 
Michel, K. (1958): Die Reifeteilungen (Meiose) bei der Spermatogenese der Schnarrheuschrecke (Psophus stridulus l.) Institut für den Wissenschaftlichen Film, Göttingen
description: Time lapse movie of meiosis II in spermatocytes of the Locust Psophus stridulus showing two sequences that include metaphase and anaphase.
 This is a historic movie made in the early 40s by Kurt Michel of the Zeiss company in Jena using Zernike's newly invented phase contrast microscope. 
 Despite its early date, the movie and the others in the group remain some of the clearest examples of chromosome behavior during meiosis.
DOI: 10.6075/j0w958cr
title: CIL:10713, Notophthalmus viridescens, oocyte. In Cell Image Library

description: Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 10 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 45 degrees. The image is grouped with one of the same sample area recorded at a 55 degree tilt. A stereo examination of the pair provides an oblique 3D view.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j0qv3kvz
title: CIL:40165, Mus musculus, peripheral neuron, myelinating Schwann cell. In Cell Image Library

description: Tissue was obtained from a postnatal day 3 male mouse. Images were obtained using a JEOL 4000 microscope. Single tilt tomogram spanned -60 to 60° at 2° increments. Magnification: 25000X, accelerating voltage: 400.0 KeV.
description: Single slice through a single tilt tomogram of the Node of Ranvier from mouse sciatic nerve prepared by high pressure freezing and freeze substitution of aldehyde fixed material. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Sosinsky et al. (2005) Development of a model for microphysiological simulations: small nodes of ranvier from peripheral nerves of mice reconstructed by electron tomography. Neuroinformatics. 3(2):133-62. PMID: 15988042
description: Preparation: crosslinking-fixative fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Data qualification: Processed
DOI: 10.6075/j0wm1cgx
title: CIL:2810, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j0s1819d
title: CIL:35968, Cavia porcellus, sperm. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Visualization methods: osmium tetroxide; uranyl salt
Data qualification: Processed
description: Figures 335 (upper) and 336 (lower) from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Upper, cross-section of guinea pig sperm tail at the level of the midpiece; lower, cross-section at the level of the principal piece. Both samples were prepared using aldehyde fixation in the presence of tannic acid. When followed with osmium and uranyl acetate staining, this procedure improves contrast but may result in thickening of the doublets, arms and spokes. Upper image by Daniel Friend; lower from David Phillips. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j00z72mn
title: CIL:39343, Helianthus annuus. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating
Processing history: unprocessed raw data
description: Instrument: FEI XL-30 FEG ESEM. Technical protocol available at http://remf.dartmouth.edu:8080/EM-Wiki/36
description: Scanning electron microscope image of sunflower lower leaf surface. Note the stomata (pore used for gas exchange) near the center of the image. This is part of an image series containing images CIL:39341-39343, and 39345.
DOI: 10.6075/j0j67gfr
title: CIL: 52317, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: Tile x=4, y=2, z=19 from BL_024216_Myc_Terc
description: One z layer of a z-stack at the given tile position
DOI: 10.6075/j0h1321v
title: CIL:39640, Saccharomyces cerevisiae. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo
PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage, automatic focusing, motorized illumination shutters and a CoolSNAP_HQ2 camera.
description: 10-hour timelapse of the double mutant Clb2dbŒî, Clb5Œî, which has the destruction box region of the CLB2 gene, on the cell cycle control system in budding yeast. The yeast, Saccharomyces cerevisia, are growing in 2% raffinose, since this mutation is inviable in glucose. The images from two experiments done with Wild-type Saccharomyces cerevisiae as a control are available as CIL:39606-39625 and CIL:39646-39665. The images from two experiments done with the double mutant Clb2dbŒî, Clb5Œî are available as CIL:39626-39645 and CIL:39666-39685.
DOI: 10.6075/j0h13100
title: CIL:36039, damselfly, flight muscle cell. In Cell Image Library

description: Negatives scanned at LBL then reduced in size for upload TEM magnifications not currently available. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron. 

Heads and tail were removed, the thorax was bisected and immersed in 100 mM KCl, 10 mM EDTA, 10 Triton X for a few hrs, to depolarize and skin the fibers, then maintained at -20 C in 50% glycerol in the same solution for several weeks to induce rigor by eliminating ATP. The muscle was gently homogenized in a waring blender in 0.9% NaCl. The fibrils were deposited on glass and sequentially fixed in 0.1% glutaraldehyde in 0.1 M cacodylate buffer, rinsed in 100 mM ammonium acetate, treated with 1% uranyl acetate for 30 seconds, rinsed in 40% methanol and frozen in liquid nitrogen. The fibrils were freeze dried at -90 C for 30 minutes and rotary shadowed with platinum either at 25C or 45C in a Balzer's 400 freeze fracture. The glass was dissolved in hydrofluoric acid and the replicas were mounted on copper grids and examined in a Philips 410 Microscope.
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: unembedded tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: The image shows the surface of the ribbon-like myofibrils, with some remnants of the SR attached mostly at the Z lines. The regular disposition of attached cross bridges is visible in many areas. Most images of this group show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. 
 
References for the image: 
Loesser, K.E. and Franzini-Armstrong, C. A simple method for freeze-drying of macromolecules and macromolecular complexes. J. Struct. Biol. 103: 48-56, 1990. Bard, F., Franzini-Armstrong, C. and Ip W. Rigor cross bridges are double headed in fast muscle from crayfish. J. Cell Biol. 105: 2225-2234, 1987.
DOI: 10.6075/j0db80gx
title: CIL:35184, Danio rerio, early embryonic cell. In Cell Image Library

description: 3 hr zebrafish embryo labeled with anti-phosphotyrosine antibody (green) and anti-phosphoserine (red). Images made available by Mark Cooper thru The Zebrafish A Living Laboratory.
description: Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Processing history: projection; rotation
DOI: 10.5281/zenodo.17490485
title: Korea Welfare Panel Study (2014-2023) by KoWePS

description: Korea Welfare Panel Study (2014-2023) by KoWePS
DOI: 10.5281/zenodo.17490486
title: Korea Welfare Panel Study (2014-2023) by KoWePS

description: Korea Welfare Panel Study (2014-2023) by KoWePS
DOI: 10.6075/j0ff3s7r
title: CIL: 54710, Bacteria E coli, MC1061. In Cell Image Library

description: mEos3.2 with TIR-STD site, scale bar 4 um.
description: Live cell bacterial imaging, expressing fluroescent protein in the cytoplasm.
description: Imaging mode: EPI on a Nikon TiE2 Hamamatsu ORCA Flash 4.0 sCMOS Camera and Nikon SR Apo TIRF 100x objective lens (NA 1.49)
DOI: 10.6075/j0tm79gr
title: CIL:39323, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Vorticella convallaria fixed for EM and buffered in cacodylate. The rough ER is not vesiculated as it is in collidine buffered fixatives but appears as sheets from which Golgi dictyosomes arise. A food vacuole is surrounded by primary lysosomes or cupulas. At the surface a continuous sheet of ER lies against the myonemes. TEM taken on 2/11/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).