cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20846916
title: Exopolysaccharides from Klebsiella oxytoca: anti-inflammatory activity

description: Abstract Exopolysaccharides (EPS) produced by Klebsiella oxytoca are of environmental, pharmaceutical, and medicinal interest. However, studies about the anti-inflammatory activity of EPS produced by this microorganism still remain limited. The aim of this study was to produce, characterize, and evaluate the anti-inflammatory activity of EPS from K. oxytoca in a pleurisy model. Colorimetric analysis revealed that precipitated crude exopolysaccharides (KEPSC) and deproteinated exopolysaccharides (KEPS) present high levels of total carbohydrates (65.57% and 62.82%, respectively). Analyses of uronic acid (7.90% in KEPSC and 6.21% in KEPS) and pyruvic acid (3.01% in KEPSC and 1.68% in KEPS) confirm that the EPS are acidic. Gas chromatography-mass spectrometry analyses demonstrated that the EPS consisted of rhamnose (29.83%), glucose (11.21%), galactose (52.45%), and mannose (6.50%). The treatment of an experimental pleurisy model in rats through subcutaneous administration of 50, 100, 200, and 400 mg/kg of KEPS decreased both the volume of inflammatory exudate and the number of leukocytes recruited to the pleural cavity. The present data showed that EPS production by K. oxytoca using the method described is easy to perform and results in a good yield. In addition, we show that KEPS exhibit anti-inflammatory activity when administered subcutaneously in rats.
DOI: 10.6084/m9.figshare.20846949
title: Resistance Exercise Training Mitigates Left Ventricular Dysfunctions in Pulmonary Artery Hypertension Model

description: Abstract Background The right ventricular hypertrophy and dilation observed in pulmonary artery hypertension (PAH) damages the left ventricle (LV) dynamics by flattening the interventricular septum. Objective To investigate whether low- to moderate-intensity resistance exercise training (RT) is beneficial to LV and cardiomyocyte contractile functions in rats during the development of monocrotaline (MCT)-induced PAH. Methods Male Wistar rats (Body weight: ~ 200 g) were used. To assess the time to potential heart failure onset (i.e., end point), rats were divided into sedentary hypertension until failure (SHF, n=6) and exercise hypertension until failure (EHF, n=6) groups. To test RT effects, rats were divided into sedentary control (SC, n = 7), sedentary hypertension (SH, n=7), and exercise hypertension (EH, n=7) groups. PAH was induced by two MCT injections (20 mg/kg, with 7 days interval). Exercise groups were submitted to an RT protocol (Ladder climbing; 55-65% of carrying maximal load), 5 times/week. Statistical significance was assumed at P &lt; 0.05. Results RT prolonged the end point (~25 %), enhanced the physical effort tolerance (~ 55%), and mitigated the LV and cardiomyocyte contractility dysfunctions promoted by MCT by preserving the ejection fraction and fractional shortening, the amplitude of shortening, and the velocities of contraction and relaxation in cardiomyocytes. RT also prevented increases in left ventricle fibrosis and type I collagen caused by MCT, and maintained the type III collagen and myocyte dimensions reduced by MCT. Conclusion Low- to moderate-intensity RT benefits LV and cardiomyocyte contractile functions in rats during the development of MCT-induced PAH.
DOI: 10.6084/m9.figshare.20846949.v1
title: Resistance Exercise Training Mitigates Left Ventricular Dysfunctions in Pulmonary Artery Hypertension Model

description: Abstract Background The right ventricular hypertrophy and dilation observed in pulmonary artery hypertension (PAH) damages the left ventricle (LV) dynamics by flattening the interventricular septum. Objective To investigate whether low- to moderate-intensity resistance exercise training (RT) is beneficial to LV and cardiomyocyte contractile functions in rats during the development of monocrotaline (MCT)-induced PAH. Methods Male Wistar rats (Body weight: ~ 200 g) were used. To assess the time to potential heart failure onset (i.e., end point), rats were divided into sedentary hypertension until failure (SHF, n=6) and exercise hypertension until failure (EHF, n=6) groups. To test RT effects, rats were divided into sedentary control (SC, n = 7), sedentary hypertension (SH, n=7), and exercise hypertension (EH, n=7) groups. PAH was induced by two MCT injections (20 mg/kg, with 7 days interval). Exercise groups were submitted to an RT protocol (Ladder climbing; 55-65% of carrying maximal load), 5 times/week. Statistical significance was assumed at P &lt; 0.05. Results RT prolonged the end point (~25 %), enhanced the physical effort tolerance (~ 55%), and mitigated the LV and cardiomyocyte contractility dysfunctions promoted by MCT by preserving the ejection fraction and fractional shortening, the amplitude of shortening, and the velocities of contraction and relaxation in cardiomyocytes. RT also prevented increases in left ventricle fibrosis and type I collagen caused by MCT, and maintained the type III collagen and myocyte dimensions reduced by MCT. Conclusion Low- to moderate-intensity RT benefits LV and cardiomyocyte contractile functions in rats during the development of MCT-induced PAH.
DOI: 10.6084/m9.figshare.20846959
title: Leaf inoculation of Azospirillum brasilense and Trichoderma harzianum in hydroponic arugula improve productive components and plant nutrition and reduce leaf nitrate

description: ABSTRACT Using beneficial fungi and bacteria to plant growth may reduce the leaf nitrate content and improve the quality of produced food. This study aimed to evaluate the isolated and combined effect of inoculation with Azospirillum brasilense and Trichoderma hazianum at two electrical conductivities on the nutrition and production of hydroponic arugula cultivation. The experiment was designed in randomized blocks, in a 4 x 2 factorial scheme, with five replications. The treatments consisted of inoculations (non-inoculated, A. brasilense, T. harzianum and co-inoculation) and two electrical conductivities (1.4 and 1.6 dS m-1). The isolated inoculation of T. harzianum and A. brasilense produced a higher root fresh mass, while the leaf chlorophyll index was higher with the inoculation of A. brasilense, concerning the other treatments. The inoculation of A. brasilense reduced the nitrate content in the arugula leaves. The inoculations and co-inoculation of A. brasilense and T. harzianum improved the yield components and plant nutrition, reduced the leaf nitrate content and promoted the biofortification of arugula leaves with Zn and Fe. In addition, the inoculation with T. harzianum increased the P and S leaf content.
DOI: 10.6084/m9.figshare.20846959.v1
title: Leaf inoculation of Azospirillum brasilense and Trichoderma harzianum in hydroponic arugula improve productive components and plant nutrition and reduce leaf nitrate

description: ABSTRACT Using beneficial fungi and bacteria to plant growth may reduce the leaf nitrate content and improve the quality of produced food. This study aimed to evaluate the isolated and combined effect of inoculation with Azospirillum brasilense and Trichoderma hazianum at two electrical conductivities on the nutrition and production of hydroponic arugula cultivation. The experiment was designed in randomized blocks, in a 4 x 2 factorial scheme, with five replications. The treatments consisted of inoculations (non-inoculated, A. brasilense, T. harzianum and co-inoculation) and two electrical conductivities (1.4 and 1.6 dS m-1). The isolated inoculation of T. harzianum and A. brasilense produced a higher root fresh mass, while the leaf chlorophyll index was higher with the inoculation of A. brasilense, concerning the other treatments. The inoculation of A. brasilense reduced the nitrate content in the arugula leaves. The inoculations and co-inoculation of A. brasilense and T. harzianum improved the yield components and plant nutrition, reduced the leaf nitrate content and promoted the biofortification of arugula leaves with Zn and Fe. In addition, the inoculation with T. harzianum increased the P and S leaf content.
DOI: 10.6084/m9.figshare.20846985
title: The Dilemmas of Growing with Purpose: The Mancha Orgânica Case

description: ABSTRACT The case presents the dilemma faced in November 2018 by Mancha Orgânica, a startup from Rio de Janeiro that produces organic paints. Mancha Orgânica is guided by principles of sustainability, producing a non-toxic paint from natural elements. Despite the recognition and good commercial results, the founding partners Amon and Pedro along with P&D consultant Martina wondered how to stimulate growth without losing the purpose of a more conscious consumption, considering the productive challenges. The case of Mancha Orgânica aims to propose a discussion about purpose and the challenges faced by purpose-driven companies. The case can be used in the disciplines of Branding or Marketing of lato or stricto sensu graduate programs. With the case, students are expected to develop the necessary skills to examine and differentiate the positioning and challenges of purpose-driven companies.
DOI: 10.6084/m9.figshare.20846985.v1
title: The Dilemmas of Growing with Purpose: The Mancha Orgânica Case

description: ABSTRACT The case presents the dilemma faced in November 2018 by Mancha Orgânica, a startup from Rio de Janeiro that produces organic paints. Mancha Orgânica is guided by principles of sustainability, producing a non-toxic paint from natural elements. Despite the recognition and good commercial results, the founding partners Amon and Pedro along with P&D consultant Martina wondered how to stimulate growth without losing the purpose of a more conscious consumption, considering the productive challenges. The case of Mancha Orgânica aims to propose a discussion about purpose and the challenges faced by purpose-driven companies. The case can be used in the disciplines of Branding or Marketing of lato or stricto sensu graduate programs. With the case, students are expected to develop the necessary skills to examine and differentiate the positioning and challenges of purpose-driven companies.
DOI: 10.34740/kaggle/dsv/4155000
title: AutoXGB
DOI: 10.6084/m9.figshare.20677106
title: MicroCT evaluation for CAD/CAM occlusal veneer fit using two materials and three cement space settings

description: Abstract This study was aimed to evaluate the fit of occlusal veneer restoration for two CAD/CAM materials with different cement space settings, using microCT scans. Sixty resin dies were made and divided into two groups (n=30) according to the materials, (I): Hybrid all-ceramic, and (II): zirconia-reinforced lithium silicate glass-ceramic. Each group was subdivided into three subgroups (n=10) according to the cement space parameters (30, 40, and 50 µm). Occlusal veneers for the six subgroups were milled. A circle with 20 different sections was placed at the center of every scanned specimen to measure four different locations (Occlusal, Axial, Marginal, and Absolute marginal discrepancy). Data were analyzed using two-way ANOVA at a 0.05 level of significance. There was no statistically significant effect of material type on the mean values of internal and marginal gaps for the three cement space parameters (P&gt;0.05). There were no statistically significant differences in the occlusal and axial gap between the cement space parameters, furthermore, there were statistically significant differences in marginal gap distances and absolute marginal discrepancies (P&gt;0.05). Hybrid all-ceramic showed smaller marginal and internal discrepancies than zirconia-reinforced lithium silicate glass-ceramic without statistically significant differences, and, for both materials, 50 µm cement space significantly improved the marginal fit and absolute marginal discrepancy.
DOI: 10.6084/m9.figshare.20677106.v1
title: MicroCT evaluation for CAD/CAM occlusal veneer fit using two materials and three cement space settings

description: Abstract This study was aimed to evaluate the fit of occlusal veneer restoration for two CAD/CAM materials with different cement space settings, using microCT scans. Sixty resin dies were made and divided into two groups (n=30) according to the materials, (I): Hybrid all-ceramic, and (II): zirconia-reinforced lithium silicate glass-ceramic. Each group was subdivided into three subgroups (n=10) according to the cement space parameters (30, 40, and 50 µm). Occlusal veneers for the six subgroups were milled. A circle with 20 different sections was placed at the center of every scanned specimen to measure four different locations (Occlusal, Axial, Marginal, and Absolute marginal discrepancy). Data were analyzed using two-way ANOVA at a 0.05 level of significance. There was no statistically significant effect of material type on the mean values of internal and marginal gaps for the three cement space parameters (P&gt;0.05). There were no statistically significant differences in the occlusal and axial gap between the cement space parameters, furthermore, there were statistically significant differences in marginal gap distances and absolute marginal discrepancies (P&gt;0.05). Hybrid all-ceramic showed smaller marginal and internal discrepancies than zirconia-reinforced lithium silicate glass-ceramic without statistically significant differences, and, for both materials, 50 µm cement space significantly improved the marginal fit and absolute marginal discrepancy.
DOI: 10.6084/m9.figshare.20847027
title: Evaluation of farmers’ wheat production technical efficiency based on income increasing

description: ABSTRACT: Based on the goal of income increasing, this research uses the DEA-BCC model under the input and the output orientation to estimate the differences and changes in the technical efficiency of wheat production by farmers of different scales based on the survey data of 1669 farmers’ wheat production from China’s main wheat-producing regions. Results showed that there are significant differences in the technical efficiency of farmer’s wheat production and the optimal scale of efficiency under the input and the output orientation. The relationship between farmers’ planting scale and efficiency is characterized by U-shaped curves and inverted U-shaped curves respectively. The wheat production technical efficiency of farmers in most major grain production areas is in a DEA ineffective state, and agricultural technical efficiency needs to be improved.
DOI: 10.6084/m9.figshare.20847043
title: “Advanced” or “precipitate”? About the Advanced/Open Access Model in Primary Health Care

description: Abstract The Advanced/Open Access model has been encouraged by managers and valued by Brazilian family and community medicine as a model of clinical management in the Family Health Team. This integrative review article discusses how this technology can qualify or hinder Primary Health Care. Although it helps to promote necessary changes in agendas traditionally focused on programmatic actions, the model has a strong managerialist bias. By disregarding basic premises, its implementation may result in professional suffering and alienation from the territory and integral health care, besides reinforcing the biomedical model and social medicalization. We point out ways for ‘advanced’ not to mean ‘precipitate’, highlighting that an implementation based on dialogue among workers, managers, and users seems more coherent with the literature on Advanced Access and with the national production on Welcoming in the Unified Health System.
DOI: 10.6084/m9.figshare.20847043.v1
title: “Advanced” or “precipitate”? About the Advanced/Open Access Model in Primary Health Care

description: Abstract The Advanced/Open Access model has been encouraged by managers and valued by Brazilian family and community medicine as a model of clinical management in the Family Health Team. This integrative review article discusses how this technology can qualify or hinder Primary Health Care. Although it helps to promote necessary changes in agendas traditionally focused on programmatic actions, the model has a strong managerialist bias. By disregarding basic premises, its implementation may result in professional suffering and alienation from the territory and integral health care, besides reinforcing the biomedical model and social medicalization. We point out ways for ‘advanced’ not to mean ‘precipitate’, highlighting that an implementation based on dialogue among workers, managers, and users seems more coherent with the literature on Advanced Access and with the national production on Welcoming in the Unified Health System.
DOI: 10.6084/m9.figshare.20847103
title: Asymmetric Opening of Mitochondrial Permeability Transition Pore in Mouse Brain Hemispheres: A Link to the Mitochondrial Calcium Uniporter Complex

description: Abstract The prolonged entry of large amounts of calcium into the mitochondria through the mitochondrial calcium uniporter complex (MCUC) may cause the permeability transition pore (mPTP) to open, which contributes to the pathogenesis of several diseases. Tissue-specific differences in mPTP opening due to variable expression of MCUC components may contribute to disease outcomes. We designed this study to determine differential mPTP opening in mitochondria isolated from different regions of mouse brain and kidney and to compare it with the expression of MCUC components. mPTP opening was measured using mitochondria isolated from the left/right brain hemispheres (LH/RH, respectively) and from kidney cortex/medulla, while the expression level of MCUC components was assessed from total cellular RNA. Interestingly, LH mitochondria showed less calcium-induced mPTP opening as compared to RH mitochondria at two different calcium concentrations. Conversely, mPTP opening was similar in the renal cortex and renal medulla mitochondria. However, the kidney mitochondria demonstrated bigger and faster mPTP opening as compared to the brain mitochondria. Furthermore, asymmetric mPTP opening in the LH and RH mitochondria was not associated with the expression of MCUC components. In brief, this study demonstrates thus far unreported asymmetric mPTP opening in mouse brain hemispheres that is not associated with the mRNA levels of MCUC components.
DOI: 10.6084/m9.figshare.20847103.v1
title: Asymmetric Opening of Mitochondrial Permeability Transition Pore in Mouse Brain Hemispheres: A Link to the Mitochondrial Calcium Uniporter Complex

description: Abstract The prolonged entry of large amounts of calcium into the mitochondria through the mitochondrial calcium uniporter complex (MCUC) may cause the permeability transition pore (mPTP) to open, which contributes to the pathogenesis of several diseases. Tissue-specific differences in mPTP opening due to variable expression of MCUC components may contribute to disease outcomes. We designed this study to determine differential mPTP opening in mitochondria isolated from different regions of mouse brain and kidney and to compare it with the expression of MCUC components. mPTP opening was measured using mitochondria isolated from the left/right brain hemispheres (LH/RH, respectively) and from kidney cortex/medulla, while the expression level of MCUC components was assessed from total cellular RNA. Interestingly, LH mitochondria showed less calcium-induced mPTP opening as compared to RH mitochondria at two different calcium concentrations. Conversely, mPTP opening was similar in the renal cortex and renal medulla mitochondria. However, the kidney mitochondria demonstrated bigger and faster mPTP opening as compared to the brain mitochondria. Furthermore, asymmetric mPTP opening in the LH and RH mitochondria was not associated with the expression of MCUC components. In brief, this study demonstrates thus far unreported asymmetric mPTP opening in mouse brain hemispheres that is not associated with the mRNA levels of MCUC components.
DOI: 10.6084/m9.figshare.20847117
title: Ginsenoside Rd inhibits migration and invasion of tongue cancer cells through H19/miR-675-5p/CDH1 axis

description: Abstract Objective Tongue squamous cell carcinoma (TSCC) is an oral cancer, with high malignancy and frequent early migration and invasion. Only a few drugs can treat tongue cancer. Ginsenoside Rd is a ginseng extract with anti-cancer effects. Many noncoding RNAs are abnormally expressed in tongue cancer, thus influencing its occurrence and development. H19 and miR-675-5p can promote cancer cell growth. This study aimed to analyze the regulation effect of ginsenoside Rd on H19 and miR-675-5p in tongue cancer. Methodology We used CCK8 and flow cytometry to study the growth and apoptosis. Transwell assay was used to assess invasion; wound-healing assay to assess migration; and colony formation assays to test the ability of cells to form colonies. H19, miR-675-5p, and CDH1 expressions were analyzed by qPCR. E-cadherin expression was detected using western blot. CRISPR/cas9 system was used for CDH1 knockout. Results Ginsenoside Rd inhibited the growth and increased the apoptosis of SCC9 cells. Ginsenoside Rd also inhibited the migration and invasion of SCC9 cells. H19 and miR-675-5p were highly expressed, while CDH1 and E-cadherin expressions were low. H19 and miR-675-5p promoted SCC9 metastasis. In contrast, CDH1 and E-cadherin inhibited the metastasis of SCC9 cells. Bioinformatics analysis showed that miR-675-5p was associated with CDH1. H19 and miR-675-5p expressions decreased after ginsenoside Rd treatment, while CDH1 and E-cadherin expressions increased. Conclusions Ginsenoside Rd inhibits tongue cancer cell migration and invasion via the H19/miR-675-5p/CDH1 axis.
DOI: 10.6084/m9.figshare.20847117.v1
title: Ginsenoside Rd inhibits migration and invasion of tongue cancer cells through H19/miR-675-5p/CDH1 axis

description: Abstract Objective Tongue squamous cell carcinoma (TSCC) is an oral cancer, with high malignancy and frequent early migration and invasion. Only a few drugs can treat tongue cancer. Ginsenoside Rd is a ginseng extract with anti-cancer effects. Many noncoding RNAs are abnormally expressed in tongue cancer, thus influencing its occurrence and development. H19 and miR-675-5p can promote cancer cell growth. This study aimed to analyze the regulation effect of ginsenoside Rd on H19 and miR-675-5p in tongue cancer. Methodology We used CCK8 and flow cytometry to study the growth and apoptosis. Transwell assay was used to assess invasion; wound-healing assay to assess migration; and colony formation assays to test the ability of cells to form colonies. H19, miR-675-5p, and CDH1 expressions were analyzed by qPCR. E-cadherin expression was detected using western blot. CRISPR/cas9 system was used for CDH1 knockout. Results Ginsenoside Rd inhibited the growth and increased the apoptosis of SCC9 cells. Ginsenoside Rd also inhibited the migration and invasion of SCC9 cells. H19 and miR-675-5p were highly expressed, while CDH1 and E-cadherin expressions were low. H19 and miR-675-5p promoted SCC9 metastasis. In contrast, CDH1 and E-cadherin inhibited the metastasis of SCC9 cells. Bioinformatics analysis showed that miR-675-5p was associated with CDH1. H19 and miR-675-5p expressions decreased after ginsenoside Rd treatment, while CDH1 and E-cadherin expressions increased. Conclusions Ginsenoside Rd inhibits tongue cancer cell migration and invasion via the H19/miR-675-5p/CDH1 axis.