DOI: 10.5061/dryad.m4d38rp
title: Data from: A mouse model of cochlear implantation with chronic electric stimulation

description: Objectives: Cochlear implants provide an effective treatment option for
 those with severe hearing loss, including those with preserved low
 frequency hearing. However, certain issues can reduce implant efficacy
 including intracochlear tissue response and delayed loss of residual
 acoustic hearing. We describe a mouse model of cochlear implantation with
 chronic electric stimulation that can be used to study cochlear implant
 biology and related pathologies. Methods: Twelve normal hearing adult
 CBA/J mice underwent unilateral cochlear implantation and were evenly
 divided into one group receiving electric stimulation and one not. Serial
 impedance and neural response telemetry (NRT) measurements were made to
 assess implant functionality. Functionality was defined as having at least
 one electrode with an impedance  35 kOhms. Mouse cochleae were harvested
 for histology and 3D X-ray microscopy 21 days post-operatively, or, in
 case the implant was still functional, at a later time point when the
 implant failed. A separate experiment measured the hearing preservation
 rate in 7 adult CBA/J mice undergoing unilateral cochlear implantation
 with serial auditory brainstem response (ABR) and distortion product
 otoacoustic emissions (DPOAE). Results: Implants maintained functionality
 for a mean of 35 days in the non-stimulated group and 19.8 days in the
 stimulated group. Reliable NRT and behavioral responses to electric
 stimulation were recorded. A robust intracochlear peri-implant tissue
 response with neo-ossification was seen in all cochleae. Six of seven mice
 maintained intact low frequency hearing up to 6 weeks following cochlear
 implantation. Conclusions: We demonstrate the feasibility of cochlear
 implantation and behaviorally significant electric stimulation in the
 mouse, with the potential for hearing preservation. This model may be
 combined with established mouse models of hearing loss and the large
 genetic and molecular research toolkit unique to the mouse for mechanistic
 and therapeutic investigations of cochlear implant biology.
description: ABR and
 DPOAEData for hearing
 preservation experimentsfibrosis
 quantificationQuantified 3D volume
 segmentation dataImpedance and
 NRTImpedance and NRT data
 for chronic stimulation experimentsHistologyRaw Histology ImagesWire breaksIn-vivo x-ray images of CINon-stimulated 3D Xray MS5-6Non-simtulated 3D Xray MS5-6.zipNon-stimulated 3D Xray MS5-5Non-stimulated 3D Xray MS5-8Non-stimulated 3D Xray MS5-8 raw imageNon-simtulated 3D Xray MS5-8.zipNon-simtulated 3D Xray MS5-7Non-stimulated 3D Xray MS5-2 part 1Non-stimulated 3D Xray MS5-2 part 1/2, file split by HJSplit and can be combined in that program. Raw imageNon-simtulated 3D Xray MS5-2.zip.001Non-stimulated 3D Xray MS5-2 part 2Non-stimulated 3D Xray MS5-2 part 2/2 - file split in HJSplit, can be combined in same program, raw imageNon-simtulated 3D Xray MS5-2.zip.002Stimulated 3D X-ray MS5-17Stimulated 3D X-ray MS5-17 raw imagebjg0080_s2_cochlea_mouse_no wire_MS5-17_80keV_2.5um_recon.zipStimulated 3D X-ray MS5-13 part 1/2Stimulated 3D X-ray MS5-13 part 1/2 split with HJSplit, can be combined with this program. raw imagebjg0070_s2_cochlea_mouse_no wire_MS5-13_80keV_2.5um_recon.zip.001Stimulated 3D X-ray MS5-13 part 2/2Stimulated 3D X-ray MS5-13 part 2/2, split with HJSplit program, can be recombined with this program, raw imagebjg0070_s2_cochlea_mouse_no wire_MS5-13_80keV_2.5um_recon.zip.002Stimulated 3D X-ray MS5-15 part 1/2Stimulated 3D X-ray MS5-15 part 1/2 split file with HJSplit, can be combined with this program, raw imagebjg0075_s2_cochlea_mouse_no wire_MS5-15_80keV_2.5um_recon.zip.001Stimulated 3D X-ray MS5-15 part 2/2Stimulated 3D X-ray MS5-15 part 2/2, file splitw ith HJSplit, can be combined with this program, raw imagebjg0075_s2_cochlea_mouse_no wire_MS5-15_80keV_2.5um_recon.zip.002Stimulated 3D X-ray MS5-19 part 1/2Stimulated 3D X-ray MS5-19 part 1/2 raw image, split by HJSplitbjg0078_s3_cochlea_mouse_no wire_MS5-19_80keV_2.5um_recon.zip.001Stimulated 3D X-ray MS5-19 part 2/2Stimulated 3D X-ray MS5-19 part 2/2 raw image, split with HJSplitbjg0078_s3_cochlea_mouse_no wire_MS5-19_80keV_2.5um_recon.zip.002Stimulated 3D X-ray MS5-20 part 1/2Stimulated 3D X-ray MS5-20 part 1/2 raw image, split with HJSplitbjg0079_s3_cochlea_mouse_no wire_MS5-20_80keV_2.5um_recon.zip.001Stimulated 3D X-ray MS5-20 part 2/2Stimulated 3D X-ray MS5-20 part 2/2 raw image, split with HJSplitbjg0079_s3_cochlea_mouse_no wire_MS5-20_80keV_2.5um_recon.zip.002Stimulated 3D X-ray MS5-16 part 1/2Stimulated 3D X-ray MS5-16 part 1/2 raw image, split with HJSplitbjg0081_s1_cochlea_mouse_no wire_MS5-16_80keV_2.5um_recon.zip.001Stimulated 3D X-ray MS5-16 part 2/2Stimulated 3D X-ray MS5-16 part 2/2 raw image, split with HJSplitbjg0081_s1_cochlea_mouse_no wire_MS5-16_80keV_2.5um_recon.zip.002