DOI: 10.5061/dryad.mw6m90650
title: Data from: Water availability and temperature as modifiers of evaporative water loss in tropical frogs

description: Water plays a notable role in the ecology of most terrestrial organisms
 due to the risks associated with water loss. Specifically, water loss in
 terrestrial animals happens through evaporation across respiratory tissues
 or epidermis. Amphibians are ideal systems for studying how abiotic
 factors impact water loss since their bodies often respond quickly to
 environmental changes. While the effect of temperature on water loss is
 well known across many taxa, we are still learning how temperature in
 combination with humidity or water availability affects water loss. Here,
 we tested how standing water sources (availability) and temperature (26
 and 36°C) together affect water loss in anuran amphibians using a Bayesian
 framework. We also present a conceptual model for considering how water
 availability and temperature may interact, resulting in body mass changes.
 After accounting for phylogenetic and time autocorrelation, we determined
 how different variables (water loss and uptake rates, temperature, and
 body size) affect body mass in three species of tropical frogs (Rhinella
 marina, Phyllobates terribilis, and Xenopus tropicalis). We found that all
 variables impacted body mass changes with greater similarities between P.
 terribilis and X. tropicalis, but only temperature showed a notable effect
 in P. terribilis. Furthermore, we describe how the behavior of P.
 terribilis might affect its water budget. This study shows how organisms
 might manage water budgets across different environments and is important
 for developing our models of evaporative water loss and species
 distributions.
description: The dataset was collected at Stanford University as described in
 doi.org/10.1093/icb/icae057. The dataset contains body mass data
 corresponding to frogs exposed to various treatments, along with
 associated metadata. The dataset was processed by imputing some missing
 values, fitting a longitudinal phylogenetic linear mixed model in a
 Bayesian framework, and plotting the raw data, results, and
 diagnostics. 
description: # Data and code for Water availability and temperature as modifiers of
 evaporative water loss in tropical frogs This repository contains 12 files
 total. The dataset contains 3 raw data and metadata files
 (amph_shl_new_Consensus_7238.tre, trees.zip, and raw_dehydration.csv.zip,
 raw_dehydration_METADATA.txt), 2 analysis files (analysis_github.R,
 model_github.stan), and 6 files for plotting data and results
 (fit.RDS.zip, res.RData, write_up12github.qmd, plots_github.R, Fig_1.R,
 plot_data.R).  ## Description of the data and file structure The tree
 files (.tre and .nex within trees.zip) were obtained
 from [https://vertlife.org/phylosubsets/](https://vertlife.org/phylosubsets/). raw_dehydration.csv.zip contains newly generated data. The two analysis files output the model results and associated diagnostics, summaries, and plots (two exceptions to this are found at the end of this section). fit.RDS.zip contains the posterior distributions resulting from the Bayesian analysis, res.RData is a summary of fit.RDS.zip. write_up12github.qmd is a summary of the model fit. plots_github.R contains relevant diagnostic and effects plots.  plot_data.R takes raw_dehydration.csv.zip as input to plot the raw data. Fig_1.R is a standalone conceptual figure with no inputs. ## Sharing/Access information Data was derived from the following sources: * [https://vertlife.org/phylosubsets/](https://vertlife.org/phylosubsets/) ## Code/Software All analyses use R. The .stan file is called within analysis_github.R. Versions of all packages are described in the associated manuscript.