cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ktx4m-yby20
title: JV1644 Legionella thyA ΔdotO

description: JV1644 Legionella thyA ΔdotO. Lacks dotO ATPase.
description: <strong>Tilt Series Date:</strong> 2016-06-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0.</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the JV1644 strain at OD~2.9. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-06-14-20/rawdata/</p>DG1644_150620160020.mrc,  Tilt Series (Pixel Size 3.9 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-06-14-20/rawdata/DG1644_150620160020.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG1644_150620160020.rec,  Reconstruction (Pixel Size 15.6 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-06-14-20/3dimage_75622/DG1644_150620160020.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0a1b9-0yy21
title: JV5443

description: JV5443 Legionella (Super-deletion+CDHFG), no Thy
description: <strong>Tilt Series Date:</strong> 2016-08-09</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate and cysteine hydrochloride  (no thymidine). Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0.</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the JV5443 strain at OD~3.2. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-17/rawdata/</p>JV5443a0069.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-17/rawdata/JV5443a0069.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV5443a0069_full.rec,  Reconstruction (Pixel Size 1.68 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-17/3dimage_77608/JV5443a0069_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r50j8-vj512
title: H-pylori_dFliM_GC

description: These are fast tilt series taken by Georges Chreifi on the Krios for dFliM mutant of Helicobacter  .  pylori.
description: <strong>Tilt Series Date:</strong> 2019-10-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild type cells but they . don't express complete FliP protein. Therefore, they don't assemble full flagella. We knocked out FliM protein . of the flagellar motor to study the early assembly states of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100% humidity. Cu grids. 10 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-28-3/rawdata/</p>Hpylori_FliM_004_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-28-3/rawdata/Hpylori_FliM_004_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_FliM_004_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-28-3/3dimage_107690/Hpylori_FliM_004_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ktzx6-0vm46
title: H_pylori_dFliO_GC

description: These are fast tilt series taken by Georges Chreifi on the Krios for dFliO mutant of Helicobacter  .  pylori.
description: <strong>Tilt Series Date:</strong> 2019-10-25</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . . flagella. We knocked out FliO protein of the flagellar motor to study the early assembly states . of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100% humidity. Cu grids. 10 nm . . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-25-208/rawdata/</p>Hpylori_FliO_212_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-25-208/rawdata/Hpylori_FliO_212_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_FliO_212_af_part41_1.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-25-208/3dimage_107649/Hpylori_FliO_212_af_part41_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0ehx0-hn894
title: lip10-2 (+ megacomplex)

description: Exposure 1-1.3s
description: <strong>Tilt Series Date:</strong> 2020-09-29</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK2, BL21 dHisdHA RQABpCDF+EFGpRSF</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Å²,  defocus: -8.0 μm,  magnification: 11000x. </p>
<strong>Microscope:</strong> IECB Talos Artica</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition</p>
<strong>Purification / Growth Conditions / Treatment:</strong> BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex.. . Selection done with Kanamycin and Streptomycin.. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane . fractions. . Liposomes are then generated from the membrane fractions by . processing through Potter and sonicator.. </p>
<strong>Sample Preparation:</strong> Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-29/rawdata/</p>lip10-2-sq2-tg2.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-29/rawdata/lip10-2-sq2-tg2.mrc"
        > <i class="download icon"></i> Download </a></p>

        lip10-2-sq2-tg2_full.rec,  Reconstruction (Pixel Size 0.78 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-29/3dimage_115770/lip10-2-sq2-tg2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.17012347
title: اخطار المحكمة الدستورية قانون المالية 2025 نموذج
title: اخطار
title: المحكمة الدستورية
title: قانون المالية
title: اخطار المحكمة الدستورية
title: البرلمان

description: الملخص

 

تشكل المحكمة الدستورية أحد الدعائم الأساسية في النظام الدستوري الجزائري، باعتبارها الهيئة العليا المكلفة بالسهر على مدى مطابقة القوانين للدستور، ومراقبة دستورية مختلف النصوص. ومن أبرز مهامها الجديدة، حق الإخطار، إذ أقرّ المشرع الجزائري آلية الإخطار كوسيلة قانونية تمكّن الهيئات المخولة، وعلى رأسها رئيس الجمهورية، من إخطار المحكمة الدستورية للنظر في مدى مطابقة مشروع قانون المالية لأحكام الدستور، وذلك وفق معايير وآليات محددة.

 

وبما أن هذه الدراسة تنصب حول "إخطار المحكمة الدستورية – قانون المالية 2025 أنموذجًا"، فقد ارتأينا تسليط الضوء على هذه الإشكالية المهمة من خلال:

 

التطرق إلى التطور التاريخي لاختصاصات المحكمة الدستورية.

 

استعراض آلية الإخطار في دستور 2020، الذي عزّز صلاحيات المحكمة الدستورية، وكرّس مبدأ الرقابة القبلية على مشاريع القوانين.

 

دراسة حالة إخطار المحكمة من طرف مجلس الأمة والمجلس الشعبي الوطني، وكذلك من طرف رئيس الجمهورية بخصوص مشروع قانون المالية 2025.

 

وقد بيّنت هذه الدراسة أن الإخطار في مجال صلاحيات المحكمة الدستورية يُعد ضمانة أساسية لتفادي إصدار قوانين غير دستورية، وفقًا لإجراءات دقيقة وفعّالة. كما أظهرت النتائج أن آلية الإخطار تساهم في ترسيخ دولة القانون، وتعزيز مبدأ سمو الدستور، وتحقيق التوازن بين السلطات.

 

كما خلصنا إلى جملة من الملاحظات التي يمكن أن تسهم في تطوير آلية الإخطار لدى المحكمة الدستورية، خاصة فيما يتعلق بضرورة توسيع قائمة الهيئات والأشخاص المخوّلين لطلب الإخطار، إلى جانب تفعيل الآليات الإجرائية التي تضمن سرعة البتّ في الإخطارات.

 

وتؤكد الدراسة على أن المحكمة الدستورية، من خلال ممارسة صلاحية الإخطار، تُعد صمام أمان للنظام الدستوري الجزائري، وضمانة لحماية الحقوق والحريات، وتحقيق التوازن السياسي والاقتصادي والاجتما

عي داخل الدولة.
DOI: 10.5281/zenodo.17012695
title: اخطار المحكمة الدستورية قانون المالية 2025 نموذج
title: اخطار
title: المحكمة الدستورية
title: قانون المالية
title: اخطار المحكمة الدستورية
title: البرلمان

description: الملخص

 

تشكل المحكمة الدستورية أحد الدعائم الأساسية في النظام الدستوري الجزائري، باعتبارها الهيئة العليا المكلفة بالسهر على مدى مطابقة القوانين للدستور، ومراقبة دستورية مختلف النصوص. ومن أبرز مهامها الجديدة، حق الإخطار، إذ أقرّ المشرع الجزائري آلية الإخطار كوسيلة قانونية تمكّن الهيئات المخولة، وعلى رأسها رئيس الجمهورية، من إخطار المحكمة الدستورية للنظر في مدى مطابقة مشروع قانون المالية لأحكام الدستور، وذلك وفق معايير وآليات محددة.

 

وبما أن هذه الدراسة تنصب حول "إخطار المحكمة الدستورية – قانون المالية 2025 أنموذجًا"، فقد ارتأينا تسليط الضوء على هذه الإشكالية المهمة من خلال:

 

التطرق إلى التطور التاريخي لاختصاصات المحكمة الدستورية.

 

استعراض آلية الإخطار في دستور 2020، الذي عزّز صلاحيات المحكمة الدستورية، وكرّس مبدأ الرقابة القبلية على مشاريع القوانين.

 

دراسة حالة إخطار المحكمة من طرف مجلس الأمة والمجلس الشعبي الوطني، وكذلك من طرف رئيس الجمهورية بخصوص مشروع قانون المالية 2025.

 

وقد بيّنت هذه الدراسة أن الإخطار في مجال صلاحيات المحكمة الدستورية يُعد ضمانة أساسية لتفادي إصدار قوانين غير دستورية، وفقًا لإجراءات دقيقة وفعّالة. كما أظهرت النتائج أن آلية الإخطار تساهم في ترسيخ دولة القانون، وتعزيز مبدأ سمو الدستور، وتحقيق التوازن بين السلطات.

 

كما خلصنا إلى جملة من الملاحظات التي يمكن أن تسهم في تطوير آلية الإخطار لدى المحكمة الدستورية، خاصة فيما يتعلق بضرورة توسيع قائمة الهيئات والأشخاص المخوّلين لطلب الإخطار، إلى جانب تفعيل الآليات الإجرائية التي تضمن سرعة البتّ في الإخطارات.

 

وتؤكد الدراسة على أن المحكمة الدستورية، من خلال ممارسة صلاحية الإخطار، تُعد صمام أمان للنظام الدستوري الجزائري، وضمانة لحماية الحقوق والحريات، وتحقيق التوازن السياسي والاقتصادي والاجتما

عي داخل الدولة.
DOI: 10.22002/s70h6-c8g10
title: Ecoli_EPEC_8612

description: Bacterial nanotubes as described by the work of Ilan Rosenshine and Sigal Ben Yehuda labs at  .  the Hebrew university).
description: <strong>Tilt Series Date:</strong> 2020-02-14</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These cells were obtained from Ritesh (Ilan Rosenshine lab) and Amit (Sigal ben Yehuda lab, . both from the Hebrew university). They were present during sample preparation.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> An overnight culture from -80C stock was made in 2ml LB and was grown at 37C in static conditions. The next day, 1 ml of cells were spun down at 4000 rpm for six minutes and . concentrated 10 times. 3 ul of cells were spotted on glow-. discharged grids located on a DMEM high-glucose agar plates (1.5% agar) for 3 hours. Then, the grids were washed twice in PBS and transferred to a starvation-medium agar plates (Starvation medium:DMEM without phenol red, vitamins and . amino acids) and incubated for 1 hour at 37C. Subsequently, the samples were washed with PBS and plunge-frozen.</p>
<strong>Sample Preparation:</strong> The samples were hold with a tweezer from the starvation plates and washed with PBS. Blotted . . with different conditions (B</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-25/rawdata/</p>EPEC_8612_124_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-25/rawdata/EPEC_8612_124_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_124_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-25/3dimage_111090/EPEC_8612_124_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9sqnq-gab90
title: E_coli_EPEC_8612

description: The aim is to look for bacterial nanotubes (See Pal et al., 2019 Cell)
description: <strong>Tilt Series Date:</strong> 2020-02-13</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 105.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These cells were obtained from Ritesh (Ilan Rosenshine lab) and Amit (Sigal ben Yehuda lab, . both from the Hebrew university). They were present during sample preparation.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> An overnight culture from -80C stock was made in 2ml LB and was grown at 37C in static . conditions. The next day, 1 ml of cells were spun down at 4000 rpm for six minutes and . concentrated 10 times. 3 ul of cells were spotted on glow-discharged grids located on a . DMEM high-glucose agar plates (1.5% agar) for 3 hours. Then, the grids were washed twice . in PBS and transferred to a starvation-medium agar plates (Starvation medium:DMEM without . phenol red, vitamins and amino acids). and incubated for 1 hour at 37C. Subsequently,the . samples were washed with PBS and plunge-frozen. </p>
<strong>Sample Preparation:</strong> The samples were hold with a tweezer from the starvation plates and washed with PBS. Blotted . with different conditions (B</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-13-99/rawdata/</p>EPEC_8612_107_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-13-99/rawdata/EPEC_8612_107_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_107_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
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DOI: 10.22002/affnm-4w473
title: Helicobacter_Pylori_dFlgs

description: These are H. pylori cells that don't have a complete FliP. In addition, FlgS was knocked out in  .  these cells. The aim is to study the assembly of the flagellar motor and the core complex.
description: <strong>Tilt Series Date:</strong> 2019-09-22</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . flagella. We knocked out FlgS protein of the flagellar motor to study the early assembly . states of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1. 100% humidity. Cu grids. 10 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-22-9/rawdata/</p>Hpylori_dFlgS8.mrc,  Tilt Series (Pixel Size 0.4494 nm), 2.4 GB  
        
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        Hpylori_dFlgS8.mrc.mdoc,  27.9 kB  
        
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