DOI: 10.5061/dryad.gmsbcc2rq
title: Data from: Genetic analysis reveals the putative native range and widespread double‐clonal reproduction in the invasive longhorn crazy ant

description: Supporting datasets for "Genetic analysis reveals the putative native
 range and widespread double-clonal reproduction system in the invasive
 longhorn crazy ant." Clonal reproduction can provide an advantage for
 invasive species to establish as it can circumvent inbreeding depression
 which often plagues introduced populations. The world's most
 widespread invasive ant, Paratrechina longicornis, was previously
 found to display a double-clonal reproduction system, whereby both males
 and queens are produced clonally, resulting in separate male and queen
 lineages, while workers are produced sexually. Under this unusual
 reproduction mode, inbreeding is avoided in workers as they carry hybrid
 interlineage genomes. Despite the ubiquitous distribution of P.
 longicornis, the significance of this reproductive system for the
 ant's remarkable success remains unclear, as its prevalence is still
 unknown. Further investigation into the controversial native origin
 of P. longicornis is also required to reconstruct the
 evolutionary histories of double-clonal lineages. Here, we examine genetic
 variation and characterize the reproduction mode of P.
 longicornis populations sampled worldwide using microsatellites
 and mitochondrial DNA sequences to infer the ant's putative native
 range and the distribution of the double-clonal reproductive system.
 Analyses of global genetic variations indicate that the Indian
 subcontinent is a genetic diversity hotspot of this species, suggesting
 that P. longicornis probably originates from this
 geographical area. Our analyses revealed that both the inferred native and
 introduced populations exhibit double-clonal reproduction, with queens and
 males around the globe belonging to two separate, nonrecombining clonal
 lineages. By contrast, workers are highly heterozygous because they are
 first-generation interlineage hybrids. Overall, these data indicate a
 worldwide prevalence of double clonality in P. longicornis and
 support the prediction that the unusual genetic system may have
 pre-adapted this ant for global colonization by maintaining heterozygosity
 in the worker force and alleviating genetic bottlenecks.
description: # Genetic analysis reveals the putative native range and widespread
 double‐clonal reproduction in the invasive longhorn crazy ant
 [https://doi.org/10.5061/dryad.gmsbcc2rq](https://doi.org/10.5061/dryad.gmsbcc2rq) ## Description of the data and file structure Dataset: Genetic Analysis of the Longhorn Crazy Ant (Paratrechina longicornis) This dataset accompanies the manuscript "Genetic analysis reveals the putative native range and widespread double-clonal reproduction in the invasive longhorn crazy ant" (Tseng et al., 2022).  Dataset Overview The dataset contains genetic data from a worldwide sampling of the longhorn crazy ant (Paratrechina longicornis), including: 1\. Microsatellite genotypes from across 256 localities 2\. Data from reproductive individuals (queens and males) from 22 localities ### Files and variables #### Data S1: Clonal Reproduction Analysis of clonal reproduction patterns #### Data S2: Global Genotypes Comprehensive genotype data from global sampling #### Data S3: Colony mtDNA Haplotype mtDNA Haplotype information for each colony #### Data S4: List of Microsatellite Primers Details of microsatellite loci used for genotyping #### Data S5: Laboratory Genotypes Genotypes from laboratory colony experiments Queens, males and sperm samples DNA Analysis Microsatellite genotyping: 40 loci mtDNA sequencing: COI, COII, tRNA-Leu genes Detailed protocols are available in the manuscript methods section **Usage Notes** Missing data indicated by "x" or "NA"\ Empty cells in files S2 and S5 are not missing data but represent haploid individuals, which do not have two alleles in the respective columns Localities coded by region abbreviations (details in manuscript Table 1) **Citation** Citation for the original manuscript: Tseng, S.-P., Darras, H., Hsu, P.-W., Yoshimura, T., Lee, C.-Y., Wetterer, J.K., Keller, L., & Yang, C.-C. (2022). Genetic analysis reveals the putative native range and widespread double-clonal reproduction in the invasive longhorn crazy ant. Molecular Ecology. [https://doi.org/10.1111/mec.16827](https://doi.org/10.1111/mec.16827) **Data Availability** The mtDNA sequences are available in GenBank (accession numbers: MZ398110–MZ398127). ## Access information Contact Information For questions about the dataset, please contact: Shu-Ping Tseng ([shupingt@ntu.edu.tw](mailto:shupingt@ntu.edu.tw))