cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30550920.v1
title: ACT001 alleviates MASLD through gut microbiota–bile acid–FXR axis in mice

description: ACT001 is a novel sesquiterpene lactone derivative with noteworthy antineoplastic and anti-inflammatory properties. This study aimed to investigate the efficacy of ACT001 against metabolic dysfunction associated steatotic liver disease (MASLD) pathogenesis in two established murine models, and to elucidate its regulatory role in the gut microbiota–bile acid–FXR axis. A high-fat diet (HFD) and a methionine–choline-deficient (MCD) diet induced two MASLD models in C57BL/6J mice. Histological analysis was performed, biochemical indices were measured, and the expression of tight junctions and farnesoid X receptor (FXR)-related pathways was detected. Gut microbiota was assessed by 16S rDNA sequencing, while bile acids (BAs) in fecal samples were analyzed using UPLC/MS–MS. ACT001 decreased liver injury, alleviated hepatic lipid accumulation, and restored intestinal barrier integrity. Furthermore, 16S rDNA illustrated that ACT001 could partially rebalance intestinal dysbacteriosis and upregulate the relative abundance of various bacteria, especially <i>Clostridium</i> and <i>Erysipelotrichaceae</i>_<i>Clostridium</i>. Moreover, ACT001 facilitated the generation of uncombined BAs and accumulated specific BAs within the ileum, such as 23-Nor-deoxycholic acid (23-DCA) and lithocholic acid-3-sulfate (LCA-3S), which had a downregulation effect on the expression of the enteral FXR–fibroblast growth factor 15 (FGF15) pathway. ACT001 exerted a multifactorial therapeutic effect on MASLD, potentially linked to the regulation of the gut microbiota–BAs–FXR axis. Thus, it has the potential to be an effective treatment for MASLD.
DOI: 10.6084/m9.figshare.29875234
title: Effect of hypertension on clinical outcomes in ischemic colitis

description: The aim of this retrospective study was to uncover the effect of hypertension on ischemic colitis (IC). Patients with IC were divided into two groups—hypertension (HP) and nonhypertension (NHP) groups—on the basis of their history of hypertension. Clinical features, biochemical indicators, endoscopic findings, abdominal pain relief time (APRT), hospital stay, operation rate, incidence of gangrenous IC, and 30-day mortality of IC were evaluated. Factors associated with ulcers and severe pain were assessed by multivariable logistic regression analysis. A total of 213 patients were enrolled in this retrospective study. The mean age in the HP group was significantly higher than in the NHP group (mean, 68.4 years vs 60.9 years; <i>p</i> = 0.000), while no statistically significant difference in gender distribution was observed between the two groups (% female: 59.3% vs 66.4%; <i>p</i> = 0.507). Patients in the HP group were more likely to suffer from moderate (52.7% VS 37.7%; <i>p</i> = 0.036) and severe (22.0% vs 9.8%; <i>p</i> = 0.019) abdominal pain than those in the NHP group. The incidence of ulceration was significantly greater in the HP group than in the NHP group (22.0% vs 10.7%; <i>p</i> = 0.034). APRT (mean, 2.8 days vs 2.7 days; <i>p</i> = 0.585), hospital stay (mean, 6.5 days vs 6.2 days; <i>p</i> = 0.276), operation rate (3.3% vs 1.6%; <i>p</i> = 0.739), incidence of gangrenous IC (5.5% vs 2.5%; <i>p</i> = 0.430), and 30-day mortality (2.2% vs 0.8%; <i>p</i> = 0.797) of IC were not significantly different between the two groups. Hypertension worsens clinical symptoms and endoscopic findings of IC but does not affect clinical outcomes.
DOI: 10.6084/m9.figshare.29875234.v1
title: Effect of hypertension on clinical outcomes in ischemic colitis

description: The aim of this retrospective study was to uncover the effect of hypertension on ischemic colitis (IC). Patients with IC were divided into two groups—hypertension (HP) and nonhypertension (NHP) groups—on the basis of their history of hypertension. Clinical features, biochemical indicators, endoscopic findings, abdominal pain relief time (APRT), hospital stay, operation rate, incidence of gangrenous IC, and 30-day mortality of IC were evaluated. Factors associated with ulcers and severe pain were assessed by multivariable logistic regression analysis. A total of 213 patients were enrolled in this retrospective study. The mean age in the HP group was significantly higher than in the NHP group (mean, 68.4 years vs 60.9 years; <i>p</i> = 0.000), while no statistically significant difference in gender distribution was observed between the two groups (% female: 59.3% vs 66.4%; <i>p</i> = 0.507). Patients in the HP group were more likely to suffer from moderate (52.7% VS 37.7%; <i>p</i> = 0.036) and severe (22.0% vs 9.8%; <i>p</i> = 0.019) abdominal pain than those in the NHP group. The incidence of ulceration was significantly greater in the HP group than in the NHP group (22.0% vs 10.7%; <i>p</i> = 0.034). APRT (mean, 2.8 days vs 2.7 days; <i>p</i> = 0.585), hospital stay (mean, 6.5 days vs 6.2 days; <i>p</i> = 0.276), operation rate (3.3% vs 1.6%; <i>p</i> = 0.739), incidence of gangrenous IC (5.5% vs 2.5%; <i>p</i> = 0.430), and 30-day mortality (2.2% vs 0.8%; <i>p</i> = 0.797) of IC were not significantly different between the two groups. Hypertension worsens clinical symptoms and endoscopic findings of IC but does not affect clinical outcomes.
DOI: 10.6084/m9.figshare.29815011
title: Effects of proprioceptive training intervention on knee function in patients with anterior cruciate ligament reconstruction: a systematic review and meta-analysis

description: This study aimed to systematically evaluate the impact of proprioceptive training on the rehabilitation of patients after anterior cruciate ligament reconstruction (ACLR) including subjective knee functional scores, knee proprioception, knee range of motion, flexion strength and single-leg hopping ability. PubMed, Web of Science, Cochrane Library, Embase, and China National Knowledge Infrastructure were searched for randomized controlled trials (RCTs) on proprioceptive training for ACLR patients from the inception of the databases to December 2, 2024. The Cochrane Risk of Bias tool was used to assess, Grading of Recommendations Assessment, Development and Evaluation evaluated evidence levels, and the effect size combination, subgroup analysis, and sensitivity analysis were performed using Stata 17. Thirteen studies with a total of 529 patients were included. The meta-analysis indicated that proprioceptive training significantly improved subjective knee functional scores (Hedge’s <i>g</i> = 0.58, 95% CI: 0.26 to 0.9, <i>p</i> = 0.00), single-leg hopping ability (Hedge’s <i>g</i> = 0.44, 95% CI: 0.15 to 0.72, <i>p</i> = 0.00), and knee proprioception (Hedge’s <i>g</i> = −0.8, 95% CI: −1.33 to −0.28, <i>p</i> = 0.00) in ACLR patients. However, proprioceptive training did not have a significant effect on knee range of motion or flexion strength. Proprioceptive training improves subjective knee functional scores, single-leg hopping ability and proprioception but does not significantly enhance knee range of motion or flexion strength in ACLR patients. Proprioceptive training enhanced subjective functional scores, and single-leg hopping ability, and knee proprioceptive function in ACLR patients.Proprioceptive training did not affect knee range of motion or knee flexion strength.The sample size significantly moderates the effects of proprioceptive training on improving knee proprioception. Future research should focus on the impact of sample size. Proprioceptive training enhanced subjective functional scores, and single-leg hopping ability, and knee proprioceptive function in ACLR patients. Proprioceptive training did not affect knee range of motion or knee flexion strength. The sample size significantly moderates the effects of proprioceptive training on improving knee proprioception. Future research should focus on the impact of sample size.
DOI: 10.6084/m9.figshare.29815011.v1
title: Effects of proprioceptive training intervention on knee function in patients with anterior cruciate ligament reconstruction: a systematic review and meta-analysis

description: This study aimed to systematically evaluate the impact of proprioceptive training on the rehabilitation of patients after anterior cruciate ligament reconstruction (ACLR) including subjective knee functional scores, knee proprioception, knee range of motion, flexion strength and single-leg hopping ability. PubMed, Web of Science, Cochrane Library, Embase, and China National Knowledge Infrastructure were searched for randomized controlled trials (RCTs) on proprioceptive training for ACLR patients from the inception of the databases to December 2, 2024. The Cochrane Risk of Bias tool was used to assess, Grading of Recommendations Assessment, Development and Evaluation evaluated evidence levels, and the effect size combination, subgroup analysis, and sensitivity analysis were performed using Stata 17. Thirteen studies with a total of 529 patients were included. The meta-analysis indicated that proprioceptive training significantly improved subjective knee functional scores (Hedge’s <i>g</i> = 0.58, 95% CI: 0.26 to 0.9, <i>p</i> = 0.00), single-leg hopping ability (Hedge’s <i>g</i> = 0.44, 95% CI: 0.15 to 0.72, <i>p</i> = 0.00), and knee proprioception (Hedge’s <i>g</i> = −0.8, 95% CI: −1.33 to −0.28, <i>p</i> = 0.00) in ACLR patients. However, proprioceptive training did not have a significant effect on knee range of motion or flexion strength. Proprioceptive training improves subjective knee functional scores, single-leg hopping ability and proprioception but does not significantly enhance knee range of motion or flexion strength in ACLR patients. Proprioceptive training enhanced subjective functional scores, and single-leg hopping ability, and knee proprioceptive function in ACLR patients.Proprioceptive training did not affect knee range of motion or knee flexion strength.The sample size significantly moderates the effects of proprioceptive training on improving knee proprioception. Future research should focus on the impact of sample size. Proprioceptive training enhanced subjective functional scores, and single-leg hopping ability, and knee proprioceptive function in ACLR patients. Proprioceptive training did not affect knee range of motion or knee flexion strength. The sample size significantly moderates the effects of proprioceptive training on improving knee proprioception. Future research should focus on the impact of sample size.
DOI: 10.6084/m9.figshare.30579357
title: Mid-infrared photons relieve tinnitus-like behavior of mice by regulating HCN2 ion channels in the medial geniculate body

description: Subjective tinnitus is an auditory perception occurring without an external sound source. The medial geniculate body (MGB) plays a critical role in tinnitus pathology. The order of this study is to investigate whether mid-infrared (MIR) modulation of the MGB can mitigate tinnitus-like behavior in mice. RNA sequencing was employed to analyze and compare gene expression levels in the MGB of mice with tinnitus to those of control mice. Golgi staining and transmission electron microscopy were used to confirm alterations in the structural plasticity of neurons. The whole-cell patch-clamp technique was applied to assess changes in neuronal functional plasticity. MIR optical fibers were employed to modulate MGB neuronal activity. Molecular dynamics simulations were performed to investigate the regulatory effect of MIR on hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channel function. The results of this study illustrates that MIR modulation reversed the abnormal electrophysiological properties of neurons associated with tinnitus-like behavior. Furthermore, molecular dynamics simulations revealed that MIR regulates HCN channel function, reducing the increased firing frequency of MGB neurons in tinnitus mice. MIR intervention may alleviate the abnormal increase in neuronal firing frequency in the MGB of tinnitus mice by modulating HCN channel function. This regulatory mechanism involves influencing the secondary structure of HCN channels, enhancing hyperpolarization-activated current amplitude, and restoring reduced amplitudes observed in tinnitus mice.
DOI: 10.6084/m9.figshare.30579357.v1
title: Mid-infrared photons relieve tinnitus-like behavior of mice by regulating HCN2 ion channels in the medial geniculate body

description: Subjective tinnitus is an auditory perception occurring without an external sound source. The medial geniculate body (MGB) plays a critical role in tinnitus pathology. The order of this study is to investigate whether mid-infrared (MIR) modulation of the MGB can mitigate tinnitus-like behavior in mice. RNA sequencing was employed to analyze and compare gene expression levels in the MGB of mice with tinnitus to those of control mice. Golgi staining and transmission electron microscopy were used to confirm alterations in the structural plasticity of neurons. The whole-cell patch-clamp technique was applied to assess changes in neuronal functional plasticity. MIR optical fibers were employed to modulate MGB neuronal activity. Molecular dynamics simulations were performed to investigate the regulatory effect of MIR on hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channel function. The results of this study illustrates that MIR modulation reversed the abnormal electrophysiological properties of neurons associated with tinnitus-like behavior. Furthermore, molecular dynamics simulations revealed that MIR regulates HCN channel function, reducing the increased firing frequency of MGB neurons in tinnitus mice. MIR intervention may alleviate the abnormal increase in neuronal firing frequency in the MGB of tinnitus mice by modulating HCN channel function. This regulatory mechanism involves influencing the secondary structure of HCN channels, enhancing hyperpolarization-activated current amplitude, and restoring reduced amplitudes observed in tinnitus mice.
DOI: 10.6084/m9.figshare.29348152
title: Effect of regional nerve block on tourniquet-related injury in pediatric patients undergoing lower limb surgery: a randomized controlled study

description: The efficacy of regional nerve block for pain management has garnered considerable attention. However, few studies have explored their effectiveness in mitigating tourniquet-related injuries and promoting functional recovery in lower limb surgery patients. : Sixty-four pediatric patients aged 6–12 undergoing knee arthroscopic surgery under general anesthesia. All patients were randomized to receive either femoral nerve block combined with sciatic nerve block (the PNB group) or standard general anesthesia only (the control group). The primary endpoint was the incidence of tourniquet-induced hypertension. Secondary endpoints included the cumulative oral morphine equivalent consumption within 24 h post-surgery, the perioperative pain intensity, inflammation and oxidative stress levels (assessed via IL-6, IL-10, SOD, and MDA), plasma levels of circulating adipokines (FABP-4 and Apelin-13), time to first ambulation, and hospital stay duration. The PNB group showed a lower incidence of tourniquet-induced hypertension compared with the control group (33.33% vs 73.33%, relative risk [95% confidence interval] of 0.182 [0.06–0.55]; <i>p</i> = 0.004). Patients in the PNB group demonstrated a 39% reduction in oral morphine equivalent consumption at 24 h postoperatively (<i>p</i> &lt; 0.001). In comparison to the control group, the PNB group also exhibited reduced postoperative pain intensity, inflammation, and oxidative stress levels, as well as lower blood glucose fluctuation. Additionally, patients in the PNB group had a significantly shorter time to first ambulation and a shorter hospital length of stay. The present study demonstrated that combining femoral nerve block and sciatic nerve block can alleviate tourniquet-related injuries, decrease opioid consumption, facilitate a quicker and less painful recovery period. The combination of femoral and sciatic nerve blocks can alleviate tourniquet-related injuries, such as reducing tourniquet-induced hypertension, ischemia–reperfusion injury, and maintaining more stable hemodynamics.The combination of femoral and sciatic nerve blocks reduced postoperative 24-h morphine consumption and the pain intensity.Regional nerve block techniques facilitate early ambulation and discharge of patients undergoing lower limb surgery. The combination of femoral and sciatic nerve blocks can alleviate tourniquet-related injuries, such as reducing tourniquet-induced hypertension, ischemia–reperfusion injury, and maintaining more stable hemodynamics. The combination of femoral and sciatic nerve blocks reduced postoperative 24-h morphine consumption and the pain intensity. Regional nerve block techniques facilitate early ambulation and discharge of patients undergoing lower limb surgery.
DOI: 10.6084/m9.figshare.29348152.v1
title: Effect of regional nerve block on tourniquet-related injury in pediatric patients undergoing lower limb surgery: a randomized controlled study

description: The efficacy of regional nerve block for pain management has garnered considerable attention. However, few studies have explored their effectiveness in mitigating tourniquet-related injuries and promoting functional recovery in lower limb surgery patients. : Sixty-four pediatric patients aged 6–12 undergoing knee arthroscopic surgery under general anesthesia. All patients were randomized to receive either femoral nerve block combined with sciatic nerve block (the PNB group) or standard general anesthesia only (the control group). The primary endpoint was the incidence of tourniquet-induced hypertension. Secondary endpoints included the cumulative oral morphine equivalent consumption within 24 h post-surgery, the perioperative pain intensity, inflammation and oxidative stress levels (assessed via IL-6, IL-10, SOD, and MDA), plasma levels of circulating adipokines (FABP-4 and Apelin-13), time to first ambulation, and hospital stay duration. The PNB group showed a lower incidence of tourniquet-induced hypertension compared with the control group (33.33% vs 73.33%, relative risk [95% confidence interval] of 0.182 [0.06–0.55]; <i>p</i> = 0.004). Patients in the PNB group demonstrated a 39% reduction in oral morphine equivalent consumption at 24 h postoperatively (<i>p</i> &lt; 0.001). In comparison to the control group, the PNB group also exhibited reduced postoperative pain intensity, inflammation, and oxidative stress levels, as well as lower blood glucose fluctuation. Additionally, patients in the PNB group had a significantly shorter time to first ambulation and a shorter hospital length of stay. The present study demonstrated that combining femoral nerve block and sciatic nerve block can alleviate tourniquet-related injuries, decrease opioid consumption, facilitate a quicker and less painful recovery period. The combination of femoral and sciatic nerve blocks can alleviate tourniquet-related injuries, such as reducing tourniquet-induced hypertension, ischemia–reperfusion injury, and maintaining more stable hemodynamics.The combination of femoral and sciatic nerve blocks reduced postoperative 24-h morphine consumption and the pain intensity.Regional nerve block techniques facilitate early ambulation and discharge of patients undergoing lower limb surgery. The combination of femoral and sciatic nerve blocks can alleviate tourniquet-related injuries, such as reducing tourniquet-induced hypertension, ischemia–reperfusion injury, and maintaining more stable hemodynamics. The combination of femoral and sciatic nerve blocks reduced postoperative 24-h morphine consumption and the pain intensity. Regional nerve block techniques facilitate early ambulation and discharge of patients undergoing lower limb surgery.
DOI: 10.6084/m9.figshare.30921070
title: Interpretable machine learning model integrating CT radiomics, CTR, and clinical features for EGFR mutation prediction in ≤3 cm lung adenocarcinoma nodules

description: Non-invasive prediction of EGFR mutation status in lung adenocarcinoma (LUAD) is critical for treatment planning, particularly in small pulmonary nodules where tissue genotyping is limited. However, the consolidation-to-tumor ratio (CTR), a clinically relevant imaging biomarker, has rarely been incorporated into radiomics-based models. To develop and validate an interpretable CT radiomics model incorporating CTR and clinical features for predicting EGFR mutation status in LUAD patients with nodules ≤3 cm. In this retrospective study included 492 patients with pathologically confirmed LUAD who underwent preoperative non-contrast chest CT between January 2017 and December 2022. Tumors were manually segmented for radiomic feature extraction, and CTR was measured for each lesion. Radiomic textures were computed with PyRadiomics using a fixed gray-level bin width. Feature selection was performed using analysis of variance and mutual information filtering followed by RFE with a random-forest base estimator. Three random forest classifiers were constructed: a radiomics-only model, a clinical-only model, and a combined radiomics-clinical model. Model performance was assessed by AUC with 95% CI, and interpretability was evaluated using SHapley Additive exPlanations (SHAP). The combined model achieved the best performance (AUC, 0.74 [95% CI: 0.69–0.79] in training; 0.76 [95% CI: 0.66–0.85] in testing), outperforming the radiomics-only (AUC, 0.69) and clinical-only (AUC, 0.60) models in the testing cohort. CTR was the most influential feature according to SHAP analysis. A interpretable radiomics model integrating CTR and clinical features enables effective non-invasive prediction of EGFR mutation status in small LUAD nodules, supporting molecular risk stratification when tissue genotyping is unavailable.
DOI: 10.6084/m9.figshare.30921070.v1
title: Interpretable machine learning model integrating CT radiomics, CTR, and clinical features for EGFR mutation prediction in ≤3 cm lung adenocarcinoma nodules

description: Non-invasive prediction of EGFR mutation status in lung adenocarcinoma (LUAD) is critical for treatment planning, particularly in small pulmonary nodules where tissue genotyping is limited. However, the consolidation-to-tumor ratio (CTR), a clinically relevant imaging biomarker, has rarely been incorporated into radiomics-based models. To develop and validate an interpretable CT radiomics model incorporating CTR and clinical features for predicting EGFR mutation status in LUAD patients with nodules ≤3 cm. In this retrospective study included 492 patients with pathologically confirmed LUAD who underwent preoperative non-contrast chest CT between January 2017 and December 2022. Tumors were manually segmented for radiomic feature extraction, and CTR was measured for each lesion. Radiomic textures were computed with PyRadiomics using a fixed gray-level bin width. Feature selection was performed using analysis of variance and mutual information filtering followed by RFE with a random-forest base estimator. Three random forest classifiers were constructed: a radiomics-only model, a clinical-only model, and a combined radiomics-clinical model. Model performance was assessed by AUC with 95% CI, and interpretability was evaluated using SHapley Additive exPlanations (SHAP). The combined model achieved the best performance (AUC, 0.74 [95% CI: 0.69–0.79] in training; 0.76 [95% CI: 0.66–0.85] in testing), outperforming the radiomics-only (AUC, 0.69) and clinical-only (AUC, 0.60) models in the testing cohort. CTR was the most influential feature according to SHAP analysis. A interpretable radiomics model integrating CTR and clinical features enables effective non-invasive prediction of EGFR mutation status in small LUAD nodules, supporting molecular risk stratification when tissue genotyping is unavailable.