cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.26126/intervals.wyj5h2
title: Cellular transepithelial electrical resistance (TEER) [Individual measurements]

description: This dataset was generated in an in vitro study where 3 cell lines (Caco-2, HT29-MTX-E12, THP-1) were cocultured in 6.5 mm Transwell® with 0.4 µm Pore Polyester Membrane Insert to mimick the intestinal environment in order to assess the potential anti-inflammatory effects of several chemical compounds.
Transepithelial electrical resistance was measured in using chopstick electrodes (STX-2) connected to an EVOM Epithelial Voltohmmeter (World Precision Instruments, Berlin, Germany). Values are expressed either in Ω.cm2 or as a percentage to control.
This dataset includes single measurements for each sample.
DOI: 10.26126/intervals.qpd2hh
title: Cell viability assay [Individual measurements]

description: This dataset was generated in an in vitro study where 3 cell lines (Caco-2, HT29-MTX-E12, THP-1) were cocultured in 6.5 mm Transwell® with 0.4 µm Pore Polyester Membrane Insert to mimick the intestinal environment in order to assess the potential anti-inflammatory effects of several chemical compounds.
The permeability of the epithelial layer was calculated by measuring the quantity of FITC-Dextran 4kDa (FD4) in the basolateral compartment. Values are expressed either in ng/mL or as a percentage to control.
This dataset includes single measurements for each sample.
DOI: 10.26126/intervals.a5yeo2
title: IL-8 and TNFalpha release in the cellular medium [Individual measurements]

description: This dataset was generated in an in vitro study where 3 cell lines (Caco-2, HT29-MTX-E12, THP-1) were cocultured in 6.5 mm Transwell® with 0.4 µm Pore Polyester Membrane Insert to mimick the intestinal environment in order to assess the potential anti-inflammatory effects of several chemical compounds.
IL-8 and TNFalpha levels were measured from the collected medium of cells using specific ELISA kits. Values are expressed either as a concentration or as a percentage to control.
This dataset includes single measurements for each sample.
DOI: 10.26126/intervals.z2btyx
title: Permeability of the epithelial layer [Individual measurements]

description: "This dataset was generated in an in vitro study where 3 cell lines (Caco-2, HT29-MTX-E12, THP-1) were cocultured in 6.5 mm Transwell® with 0.4 µm Pore Polyester Membrane Insert to mimick the intestinal environment in order to assess the potential anti-inflammatory effects of several chemical compounds.
The cell viability was calculated using a Cell Counting Kit-8 (CCK-8, Sigma) by measuring WST-8 bioreduction in single cell lines.
This dataset includes single measurements for each sample."
DOI: 10.6084/m9.figshare.12993127
title: Bluetongue in India: a systematic review and meta-analysis with emphasis on diagnosis and seroprevalence

description: Bluetongue (BT) is an infectious viral disease which affects a wide range of ruminants and was first reported in India in 1964. In view of the absence of comprehensive information on the BT status in India, this study presents the seroprevalence on BT in farm animals of India based-on a systematic review and meta-analysis. A systematic review was conducted to identify the published articles (2001–2018) reporting the seroprevalence of BT in sheep, goats, cattle, buffalo, camels, and Mithun (<i>Bos frontalis</i>) from India. From 409 research articles, 71 fulfilled the inclusion criteria and meta-analysis for proportions was carried out targeting the eligible studies. From these, 144 strata level data were extracted with a sample size of 14048 sheep, 14696 goats, 5218 cattle, 2653 buffaloes, 2062 camels, and 222 Mithun. Overall, the analyses showed that the BT seroprevalence of 43% (95% CI: 38–49%) in goats, 39% (95% CI: 33–46%) in sheep, 38% (95% CI: 25–45%) in cattle, 34% (95% CI: 20–51%) in buffaloes, 16% (95% CI: 10–22%) in camels, and 66% (95% CI: 17–95%) in Mithun. Furthermore, the meta-regression analysis suggested that serological tests, geographical region, and sample size were the prime moderators. Meta-analytic study indicates the BT seropositivity in 25.35 million sheep (95% CI: 21.5–29.9), 58 million goats (95% CI: 51.3–66.2), 66.8 million cattle (95% CI: 47.7–86), 37.0 million buffaloes (95% CI: 21.7–55.4), 0.06 million camels (95% CI: 0.04–0.09), and 0.19 million Mithun (95% CI: 0.05–0.28). The findings highlight the variation of BT seropositivity in different geographical regions of India.
DOI: 10.6084/m9.figshare.13347125.v1
title: Effects of sex, age, and season on the variation of blood analytes in a clinically healthy <i>ex situ</i> population of bottlenose dolphins (<i>Tursiops</i> spp.)

description: A comprehensive evaluation of the effects of sex, age, and season on blood analytes in a robust population size of <i>ex situ</i> bottlenose dolphins (<i>Tursiops</i> spp.) has not been investigated to date. To define the variation in hematological and biochemical analytes of dolphins due to sex, age, and season. 1,426 blood samples collected from 156 clinically normal dolphins consisting of 59 males and 97 females in which 37 analytes were measured were retrospectively identified. The dolphins were categorized by age, sex, and season, and categories were compared. About 23 (64%) analytes differed by age. The number of differences between adjacent age groups decreased with advancing age. MPV, glucose, BUN, globulins, GGT and Cl progressively increased with age, whereas Abs lymphs, total protein, ALP, CK and Ca progressively decreased with age. Three (8%) of analytes differed between sex, whereas 16 (44%) analytes differed by season. Female dolphins had higher median iron (33 µmol/L) than male dolphins (25 µmol/L). Female dolphins also had higher Abs lymphs and MCHC, but Abs lymphs and MCHC also differed between age and season, respectively. Sex inconsistently and relatively infrequently influences analytes. Delphinids of advancing age experience immune senescence and decreasing renal perfusion or clearance. These results demonstrate the importance of considering the influences of sex, age, and season on blood data, provide a baseline for accurate interpretation of clinicopathological analytes of delphinids in managed care, and will be useful for investigations into health, disease, and stressors of wild delphinids.
DOI: 10.6084/m9.figshare.11912166
title: Emerging novel coronavirus (2019-nCoV)—current scenario, evolutionary perspective based on genome analysis and recent developments

description: Coronaviruses are the well-known cause of severe respiratory, enteric and systemic infections in a wide range of hosts including man, mammals, fish, and avian. The scientific interest on coronaviruses increased after the emergence of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) outbreaks in 2002-2003 followed by Middle East Respiratory Syndrome CoV (MERS-CoV). This decade’s first CoV, named 2019-nCoV, emerged from Wuhan, China, and declared as ‘Public Health Emergency of International Concern’ on January 30<sup>th</sup>, 2020 by the World Health Organization (WHO). As on February 4, 2020, 425 deaths reported in China only and one death outside China (Philippines). In a short span of time, the virus spread has been noted in 24 countries. The zoonotic transmission (animal-to-human) is suspected as the route of disease origin. The genetic analyses predict bats as the most probable source of 2019-nCoV though further investigations needed to confirm the origin of the novel virus. The ongoing nCoV outbreak highlights the hidden wild animal reservoir of the deadly viruses and possible threat of spillover zoonoses as well. The successful virus isolation attempts have made doors open for developing better diagnostics and effective vaccines helping in combating the spread of the virus to newer areas.
DOI: 10.6084/m9.figshare.12993127.v1
title: Bluetongue in India: a systematic review and meta-analysis with emphasis on diagnosis and seroprevalence

description: Bluetongue (BT) is an infectious viral disease which affects a wide range of ruminants and was first reported in India in 1964. In view of the absence of comprehensive information on the BT status in India, this study presents the seroprevalence on BT in farm animals of India based-on a systematic review and meta-analysis. A systematic review was conducted to identify the published articles (2001–2018) reporting the seroprevalence of BT in sheep, goats, cattle, buffalo, camels, and Mithun (<i>Bos frontalis</i>) from India. From 409 research articles, 71 fulfilled the inclusion criteria and meta-analysis for proportions was carried out targeting the eligible studies. From these, 144 strata level data were extracted with a sample size of 14048 sheep, 14696 goats, 5218 cattle, 2653 buffaloes, 2062 camels, and 222 Mithun. Overall, the analyses showed that the BT seroprevalence of 43% (95% CI: 38–49%) in goats, 39% (95% CI: 33–46%) in sheep, 38% (95% CI: 25–45%) in cattle, 34% (95% CI: 20–51%) in buffaloes, 16% (95% CI: 10–22%) in camels, and 66% (95% CI: 17–95%) in Mithun. Furthermore, the meta-regression analysis suggested that serological tests, geographical region, and sample size were the prime moderators. Meta-analytic study indicates the BT seropositivity in 25.35 million sheep (95% CI: 21.5–29.9), 58 million goats (95% CI: 51.3–66.2), 66.8 million cattle (95% CI: 47.7–86), 37.0 million buffaloes (95% CI: 21.7–55.4), 0.06 million camels (95% CI: 0.04–0.09), and 0.19 million Mithun (95% CI: 0.05–0.28). The findings highlight the variation of BT seropositivity in different geographical regions of India.
DOI: 10.6084/m9.figshare.13347125
title: Effects of sex, age, and season on the variation of blood analytes in a clinically healthy <i>ex situ</i> population of bottlenose dolphins (<i>Tursiops</i> spp.)

description: A comprehensive evaluation of the effects of sex, age, and season on blood analytes in a robust population size of <i>ex situ</i> bottlenose dolphins (<i>Tursiops</i> spp.) has not been investigated to date. To define the variation in hematological and biochemical analytes of dolphins due to sex, age, and season. 1,426 blood samples collected from 156 clinically normal dolphins consisting of 59 males and 97 females in which 37 analytes were measured were retrospectively identified. The dolphins were categorized by age, sex, and season, and categories were compared. About 23 (64%) analytes differed by age. The number of differences between adjacent age groups decreased with advancing age. MPV, glucose, BUN, globulins, GGT and Cl progressively increased with age, whereas Abs lymphs, total protein, ALP, CK and Ca progressively decreased with age. Three (8%) of analytes differed between sex, whereas 16 (44%) analytes differed by season. Female dolphins had higher median iron (33 µmol/L) than male dolphins (25 µmol/L). Female dolphins also had higher Abs lymphs and MCHC, but Abs lymphs and MCHC also differed between age and season, respectively. Sex inconsistently and relatively infrequently influences analytes. Delphinids of advancing age experience immune senescence and decreasing renal perfusion or clearance. These results demonstrate the importance of considering the influences of sex, age, and season on blood data, provide a baseline for accurate interpretation of clinicopathological analytes of delphinids in managed care, and will be useful for investigations into health, disease, and stressors of wild delphinids.
DOI: 10.6084/m9.figshare.12490079.v2
title: Some pathological observations on the naturally infected dromedary camels (Camelus dromedarius) with the Middle East respiratory syndrome coronavirus (MERS-CoV) in Saudi Arabia 2018–2019

description: The natural MERS-CoV infection in dromedary camels is understudied. Recent experimental studies showed no obvious clinical signs in the infected dromedary camels. To study the pathological changes associated with natural MERS-CoV infection in dromedary camels. Tissues from three MERS-CoV positive animals as well as two negative animals were collected and examined for the presence of pathological changes. The screening of the animals was carried out first by the rapid agglutination test and then confirmed by the RT-PCR. The selected animals ranged from six to twelve months in age. The sensitivity of the latter technique was much higher in the detection of MERS-CoV than the Rapid test (14 out of 75 animals positive or 18% versus 31 out of 75 positive or 41%). MERS-CoV induced marked desquamation of the respiratory epithelium accompanied by lamina propria and submucosal mononuclear cells infiltration, epithelial hyperplasia in the respiratory tract, and interstitial pneumonia. Ciliary cell loss was seen in the trachea and turbinate. In addition, degeneration of glomerular capillaries with the complete destruction of glomerular tufts that were replaced with fibrinous exudate in renal corpuscles in the renal cortex were noticed. Expression of the MERS-CoV-S1 and MERS-CoV-N proteins was revealed in respiratory tract, and kidneys. To our knowledge, this is the first study describing the pathological changes of MERS-CoV infection in dromedary camels under natural conditions. In contrast to experimental infection in case of spontaneous infection interstitial pneumonea is evident at least in some affected animals.
DOI: 10.6084/m9.figshare.13325877
title: Genomic characterization of enteropathogenic <i>Escherichia coli</i> (EPEC) of avian origin and rabbit ileal loop response; a pet macaw (<i>Ara chloropterus</i>) as a possible zoonotic reservoir

description: Enteropathogenic <i>Escherichia coli</i> (EPEC) constitutes one of the main causes of mortality in children in low- to medium-income countries. Diverse animal species have been linked as reservoirs, including birds. The aim of this study was to describe the genomic and phylogenetic features of an EPEC recovered from a pet macaw and further characterizing the macro and microscopic lesion in a rabbit ileal loop experimental model. The isolate was whole-genome sequenced (WGS) obtaining its genotypic and phenotypic <i>in silico</i> characteristics and inoculated in a rabbit experimental model with subsequently evaluating the strain’s pathogenicity by scanning electron microscopy (SEM) and histopathology. The isolate was characterized as O109:H21-B1-ST40 typical EPEC, harboring several virulence factors of diarrheagenic <i>E. coli</i>. The macaw EPEC genome was located in a monophyletic clade of human and animal ST40 EPEC sequences. <i>In vivo</i> inoculation demonstrated severe hemorrhage with SEM and histopathological analysis confirming these lesions to be associated with intra-epithelial lymphocytes. Therefore, the isolate not only shared several genotypic and phylogenetic similarities with EPEC that affects humans and animals, but was able to induce severe tissue injury in a mammal model. These findings highlight the underrated role of pet birds as zoonotic reservoirs and the diversity in virulence factors being unraveled by new WGS studies.
DOI: 10.6084/m9.figshare.12490079
title: Some pathological observations on the naturally infected dromedary camels (Camelus dromedarius) with the Middle East respiratory syndrome coronavirus (MERS-CoV) in Saudi Arabia 2018–2019

description: The natural MERS-CoV infection in dromedary camels is understudied. Recent experimental studies showed no obvious clinical signs in the infected dromedary camels. To study the pathological changes associated with natural MERS-CoV infection in dromedary camels. Tissues from three MERS-CoV positive animals as well as two negative animals were collected and examined for the presence of pathological changes. The screening of the animals was carried out first by the rapid agglutination test and then confirmed by the RT-PCR. The selected animals ranged from six to twelve months in age. The sensitivity of the latter technique was much higher in the detection of MERS-CoV than the Rapid test (14 out of 75 animals positive or 18% versus 31 out of 75 positive or 41%). MERS-CoV induced marked desquamation of the respiratory epithelium accompanied by lamina propria and submucosal mononuclear cells infiltration, epithelial hyperplasia in the respiratory tract, and interstitial pneumonia. Ciliary cell loss was seen in the trachea and turbinate. In addition, degeneration of glomerular capillaries with the complete destruction of glomerular tufts that were replaced with fibrinous exudate in renal corpuscles in the renal cortex were noticed. Expression of the MERS-CoV-S1 and MERS-CoV-N proteins was revealed in respiratory tract, and kidneys. To our knowledge, this is the first study describing the pathological changes of MERS-CoV infection in dromedary camels under natural conditions. In contrast to experimental infection in case of spontaneous infection interstitial pneumonea is evident at least in some affected animals.
DOI: 10.6084/m9.figshare.11912166.v1
title: Emerging novel coronavirus (2019-nCoV)—current scenario, evolutionary perspective based on genome analysis and recent developments

description: Coronaviruses are the well-known cause of severe respiratory, enteric and systemic infections in a wide range of hosts including man, mammals, fish, and avian. The scientific interest on coronaviruses increased after the emergence of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) outbreaks in 2002-2003 followed by Middle East Respiratory Syndrome CoV (MERS-CoV). This decade’s first CoV, named 2019-nCoV, emerged from Wuhan, China, and declared as ‘Public Health Emergency of International Concern’ on January 30<sup>th</sup>, 2020 by the World Health Organization (WHO). As on February 4, 2020, 425 deaths reported in China only and one death outside China (Philippines). In a short span of time, the virus spread has been noted in 24 countries. The zoonotic transmission (animal-to-human) is suspected as the route of disease origin. The genetic analyses predict bats as the most probable source of 2019-nCoV though further investigations needed to confirm the origin of the novel virus. The ongoing nCoV outbreak highlights the hidden wild animal reservoir of the deadly viruses and possible threat of spillover zoonoses as well. The successful virus isolation attempts have made doors open for developing better diagnostics and effective vaccines helping in combating the spread of the virus to newer areas.
DOI: 10.6084/m9.figshare.13325877.v1
title: Genomic characterization of enteropathogenic <i>Escherichia coli</i> (EPEC) of avian origin and rabbit ileal loop response; a pet macaw (<i>Ara chloropterus</i>) as a possible zoonotic reservoir

description: Enteropathogenic <i>Escherichia coli</i> (EPEC) constitutes one of the main causes of mortality in children in low- to medium-income countries. Diverse animal species have been linked as reservoirs, including birds. The aim of this study was to describe the genomic and phylogenetic features of an EPEC recovered from a pet macaw and further characterizing the macro and microscopic lesion in a rabbit ileal loop experimental model. The isolate was whole-genome sequenced (WGS) obtaining its genotypic and phenotypic <i>in silico</i> characteristics and inoculated in a rabbit experimental model with subsequently evaluating the strain’s pathogenicity by scanning electron microscopy (SEM) and histopathology. The isolate was characterized as O109:H21-B1-ST40 typical EPEC, harboring several virulence factors of diarrheagenic <i>E. coli</i>. The macaw EPEC genome was located in a monophyletic clade of human and animal ST40 EPEC sequences. <i>In vivo</i> inoculation demonstrated severe hemorrhage with SEM and histopathological analysis confirming these lesions to be associated with intra-epithelial lymphocytes. Therefore, the isolate not only shared several genotypic and phylogenetic similarities with EPEC that affects humans and animals, but was able to induce severe tissue injury in a mammal model. These findings highlight the underrated role of pet birds as zoonotic reservoirs and the diversity in virulence factors being unraveled by new WGS studies.
DOI: 10.5281/zenodo.4674256
title: CROSSBOW Weather data forecasts

description: Weather data forecasts are used for forecasting dynamic line rating of overhead lines. Source of data: SKIRON, the regional forecasting model created by the National & Kapodistrian University of Athens - School of Physics, Division of Physics of Environment Files contain weather forecast data (temperature, wind direction, wind speed and GHI) for 48 hours for six locations (Ernestinovo, Žerjavinec, Tumbri, Konjsko, Velebit and Melina) in Croatia.
DOI: 10.5281/zenodo.4674255
title: CROSSBOW Weather data forecasts

description: Weather data forecasts are used for forecasting dynamic line rating of overhead lines. Source of data: SKIRON, the regional forecasting model created by the National & Kapodistrian University of Athens - School of Physics, Division of Physics of Environment Files contain weather forecast data (temperature, wind direction, wind speed and GHI) for 48 hours for six locations (Ernestinovo, Žerjavinec, Tumbri, Konjsko, Velebit and Melina) in Croatia.
DOI: 10.25375/uct.12826538
title: Mammalian herbivore movement into drought refugia has cascading effects on savanna insect communities.

description: Abstract<br>1. Global climate change is predicted to increase the frequency of droughts, with major impacts on tropical savannas. It has been suggested that during drought, increased soil moisture and nutrients on termite mounds could benefit plants but it is unclear how such benefits could cascade to affect insect communities.<br>2. Here, we describe the effects of drought on vegetation structure, the cascading implications for invertebrates and how termite mounds influence such effects. We compared how changes in grass biomass affected grasshopper and ant diversity on and off Macrotermes mounds before (2012) and during a drought (2016) at two locations that experienced large variation in drought severity (Skukuza and Pretoriuskop) in the Kruger National Park, South Africa.<br>3. The 2013-2016 drought was not ubiquitous across the study site, with rainfall decreasing at Skukuza and being above average at Pretoriuskop. However, grass biomass declined at both locations. Grasshopper abundance decreased at droughted Skukuza both on and off mounds but decreased on mounds and increased off mounds at non-droughted Pretoriuskop. Ant abundance and species richness increased at Skukuza but remained the same on mounds and decreased off mounds at Pretoriuskop.<br>4. Our results demonstrate the spatially extensive effects of drought. Despite above average rainfall in 2016 at Pretoriuskop, grass biomass decreased, likely due to an influx of large mammalian herbivores from drought-affected areas. This decrease in grass biomass cascaded to affect grasshoppers and ants, further illustrating the effects of drought on invertebrates in adjoining areas with higher rainfall. Our grasshopper results also suggest that increased drought in savannas will contribute to overall declines in insect abundance. Moreover, our recorded increase in ant abundance was primarily in the form of increases in dominant species, illustrating how drought-<br>induced shifts in relative abundance will likely influence ecosystem structure and function. <br>5. Our study highlights the phenomenon of spill-over drought effects and suggests rather than mitigating drought, termite mounds can instead become the focus for more intense grazing, with important consequences for insect communities.<br>
DOI: 10.6084/m9.figshare.14392841
title: Dataset 3

description: Data of KL and KW of AM RILs in six environments
DOI: 10.6084/m9.figshare.14392841.v1
title: Dataset 3

description: Data of KL and KW of AM RILs in six environments
DOI: 10.5281/zenodo.4674258
title: Corpus of Novels of the Spanish Silver Age (CoNSSA). First release (April 2021)

description: A first release of the CoNSSA, in preparation with the publication of the thesis.
DOI: 10.5281/zenodo.4674257
title: Corpus of Novels of the Spanish Silver Age (CoNSSA). First release (April 2021)

description: A first release of the CoNSSA, in preparation with the publication of the thesis.