DOI: 10.6084/m9.figshare.22615033.v2
title: Additional file 1 of Lifestyle-, environmental-, and additional health factors associated with an increased sperm DNA fragmentation: a systematic review and meta-analysis

description: Additional file 1: Supplementary Appendix 1. Risk of bias assessment methodology. Supplementary Table 1. PRISMA 2020 checklist. Supplementary Table 2. Basic characteristics of the included article. Supplementary Table 3. Eligibility criteria in each included article. Supplementary Table 4. Risk factor and population definitions in each included article. Supplementary Table 5. Risk of bias assessment using the QUIPS tools. Supplementary Table 6. Articles also looking at pregnancy or birth as an outcome. Supplementary Figure 1. Comparison of patients’ sperm DNA fragmentation values with and without varicocele subdivided based on sperm DNA fragmentation assays used (continuous data). Supplementary Figure 2. Comparison of patients’ sperm DNA fragmentation values with and without varicocele subdivided based on different cut-off values. Supplementary Figure 3. Supplementary Figure 4. Comparison of patients’ sperm DNA fragmentation values with and without varicocele subdivided based on the fertility status of patients (continuous data). Supplementary Figure 5. Comparison of patients’ sperm DNA fragmentation values with impaired and normal glucose tolerance (continuous data). Supplementary Figure 6. Comparison of patients’ sperm DNA fragmentation values with and without testicular tumors subdivided based on sperm DNA fragmentation assays used (continuous data). Supplementary Figure 7. Comparison of patients’ sperm DNA fragmentation values with and without Hodgkin-lymphoma (HL) (continuous data). Supplementary Figure 8. Comparison of patients’ sperm DNA fragmentation values with and without non-Hodgkin lymphoma (NHL) (continuous data). Supplementary Figure 9. Comparison of patients’ sperm DNA fragmentation values with and without lymphomas (continuous data). Supplementary Figure 10. Comparison of patients’ sperm DNA fragmentation values with and without leukemia (continuous data). Supplementary Figure 11. Comparison of patients’ sperm DNA fragmentation values with and without human papilloma virus (HPV) infections (continuous data). Supplementary Figure 12. Supplementary Figure 13. Comparison of patients’ sperm DNA fragmentation values with and without viral infections (continuous data). Supplementary Figure 14. Supplementary Figure 15. Supplementary Figure 16. Comparison of smokers and non-smokers’ sperm DNA fragmentation values subdivided based on sperm DNA fragmentation assays used (continuous data). Supplementary Figure 17. Comparison of heavy smokers with non-smokers, and moderate smokers with non-smokers’ sperm DNA fragmentation values (continuous data). Supplementary Figure 18. Comparison of smokers and non-smokers’ sperm DNA fragmentation values subdivided based on the fertility status of patients (continuous data). Supplementary Figure 19. Comparison of drinkers’ and abstainers’ sperm DNA fragmentation values (continuous data). Supplementary Figure 20. Comparison of heavy drinkers with abstainers, and moderate drinkers with abstainers’ sperm DNA fragmentation values (continuous data). Supplementary Figure 21. Comparison of sperm DNA fragmentation values of patients with different BMI categories (continuous data). Supplementary Figure 22. Supplementary Figure 23. Supplementary Figure 24. Supplementary Figure 25. Supplementary Figure 26. Supplementary Figure 27. Supplementary Figure 28. Supplementary Figure 29. Supplementary Figure 30. Supplementary Figure 31. Supplementary Figure 32. Supplementary Figure 33. Supplementary Figure 34. Supplementary Figure 35. Supplementary Figure 36. Comparison of patients’ sperm DNA fragmentation values of different age groups (continuous data). Supplementary Figure 37. Comparison of patients’ sperm DNA fragmentation values of different age groups (continuous data). Supplementary Figure 38. Comparison of patients’ sperm DNA fragmentation values of different age groups with cut-off values being at 30% DNA fragmentation. Supplementary Figure 39. Comparison of patients’ sperm DNA fragmentation values of age groups over and below 40 years of age with different cut-off values for DNA fragmentation. Supplementary Figure 40. Supplementary Figure 41. Comparison of patients’ sperm DNA fragmentation values with exposures to different types of pollutants (continuous data). Supplementary Figure 42. Supplementary Figure 43. Supplementary Figure 44. Comparison of patients’ sperm DNA fragmentation values with more or less reactive oxygen species (ROS) (continuous data). Supplementary Figure 45. Comparison of patients’ sperm DNA fragmentation values with more vs. less reactive oxygen species (ROS) – reduced model (continuous data). Supplementary Figure 46. Comparison of patients’ sperm DNA fragmentation values within the risk group and control group (continuous data). Supplementary Figure 47. Comparison of patients’ sperm DNA fragmentation values within the risk group and control group (continuous data, same subjects in the two groups). Supplementary Figure 48. Comparison of patients’ sperm DNA fragmentation values within the risk group and control group (cut-off values).