cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14280408.v1
title: [Metabolic profile of captive Amazonian manatee calves (Trichechus inunguis), fed different substitutes for maternal milk]

description: ABSTRACT Ten Amazonian manatee calves who live in captivity were fed with different substitutes for maternal milk in two phases. During the first phase, the calves were fed substitute 1 (S1), which is what was routinely used by INPA, and during the second phase they were fed substitute 2 (S2), which was formulated based on the nutritional composition of T. inunguis maternal milk. Each phase lasted 62 days, when blood samples were collected for analysis of values of total protein (PT), albumin (ALB), globulin (GLO), glucose (GLI), total lipids (LT), cholesterol (COL), triglycerides (TRI), calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), hematocrit (Ht) and hemoglobin (Hb). There was a decrease in PT, ALB, GLO, Ca, P and K after feeding the animals with S2 when compared to values obtained with S1. Sodium levels were higher when S2 was administered (148.16±1.15mmol/L) than when S1 was given (143.47±0.99 mmol/l). Values of Ht and Mg did not differ between treatments. The metabolic profile was within normal levels for this species.
DOI: 10.6084/m9.figshare.14280416.v1
title: [Dispersion of lidocaine epidurally administered to dogs positioned on lateral or external decubitus]

description: ABSTRACT The aim of this study was to clinically verify the dispersion of lidocaine in the epidural space of dogs placed in different positions. Sixteen dogs with an average weight of 17.5 kilograms were used. These were tranquilized with acepromazine, anesthetized with propofol and allocated to two groups: sternal decubitus (GE) and right lateral decubitus (GLD). Both groups received 2% of lidocaine in the volume of 0.25mL/kg and remained in the same position for 20 minutes. The blocking of the pelvic limbs and the extension of it from the seventh lumbar vertebra were evaluated by means of interdigital and paravertebral panniculus clamping. Orchiectomy surgery was then performed. Afterwards, the total blocking time of the pelvic limbs was evaluated. All dogs presented bilateral blocking, with no differences in cranial extension between groups, with a median of 7.5 (1-14) vertebrae for GE and 4 (1-14) for GLD. The blocking time of the right and left limbs were 123 ± 26 and 130 ± 20 minutes for GE, and 120 ± 21 and 121 ± 20 minutes for GLD with no difference between groups or between limbs. It is concluded that the decubitus does not interfere with the epidural lidocaine distribution.
DOI: 10.6084/m9.figshare.14280414
title: [A critical-historical analysis of the continuous development of Brazilian legislation related to artisanal cheeses]

description: ABSTRACT Different artisanal cheeses are produced, commercialized and consumed in Brazil, leading to a constant development of related rules by the MAPA and other official agencies. The establishment of two national programs (SUASA and SISBI-POA) allowed an equivalence in inspection system and an expanded distribution. Also, MAPA allowed ripening time lower than 60 days for artisanal raw milk cheeses, based on scientific studies that assure their safety. However, lowering the ripening period is still controversial, once there are no proper established criteria for such scientific studies, leading to potential multiple interpretation of data. The newly established ARTE certification transferred the inspection responsibilities of artisanal products to secretaries of agriculture, livestock and health, in support of what was already predicated by MAPA and SISBI-POA. Based on ARTE certification, the inspection service must also provide orientation guidance to producers, which should be done specifically by extension organs and associations. The norms that guide the production and commercialization of these artisanal products often need to be updated, but based on well-established methodologies and procedures, to ensure the distribution of suitable products to consumers.
DOI: 10.6084/m9.figshare.14280417
title: [Efficiency of two techniques for recovering epididymal spermatozoids from dogs and seminal evaluation post cryopreservation]

description: ABSTRACT The objective of this work was to evaluate the recovery of epididymal spermatozoa from castrated dogs using retrograde flow (FL) and flotation (FL) techniques in Tris-egg yolk diluent, before and after cryopreservation. Thirty testicle-epididymal complexes (CTE) were collected, 15 for FR and 15 for FL and soon after spermatozoid recovery, morphological changes in these spermatic cells were analyzed. After addition of the diluent, the parameters of total motility (MOT) and vigor (V) were evaluated. The post-cryopreserved semen was submitted to thermoresistance (TTR) test at T0, T30, T60 and T90 minutes, as well as the plasma and acrosomal membrane evaluation by fluorescent probes. There was no statistically significant difference between techniques tested for MOT and vigor in the diluted semen (FR-MOT: 82.3% and V: 3.4, FL-MOT: 79.6% and V: 3.2) and post-cryopreserved (FR-MOT: 34% and V: 2.8, FL-MOT: 30% and V: 2.7). From the T30 there was a significant difference regarding MOT and vigor in the used techniques, and the time also damaged the spermatic acrosome from the T30. It is concluded that the epididymal spermatozoa recovering techniques from castrated dogs, tested in this study, can be used for semen refrigeration and cryopreservation.
DOI: 10.6084/m9.figshare.14280417.v1
title: [Efficiency of two techniques for recovering epididymal spermatozoids from dogs and seminal evaluation post cryopreservation]

description: ABSTRACT The objective of this work was to evaluate the recovery of epididymal spermatozoa from castrated dogs using retrograde flow (FL) and flotation (FL) techniques in Tris-egg yolk diluent, before and after cryopreservation. Thirty testicle-epididymal complexes (CTE) were collected, 15 for FR and 15 for FL and soon after spermatozoid recovery, morphological changes in these spermatic cells were analyzed. After addition of the diluent, the parameters of total motility (MOT) and vigor (V) were evaluated. The post-cryopreserved semen was submitted to thermoresistance (TTR) test at T0, T30, T60 and T90 minutes, as well as the plasma and acrosomal membrane evaluation by fluorescent probes. There was no statistically significant difference between techniques tested for MOT and vigor in the diluted semen (FR-MOT: 82.3% and V: 3.4, FL-MOT: 79.6% and V: 3.2) and post-cryopreserved (FR-MOT: 34% and V: 2.8, FL-MOT: 30% and V: 2.7). From the T30 there was a significant difference regarding MOT and vigor in the used techniques, and the time also damaged the spermatic acrosome from the T30. It is concluded that the epididymal spermatozoa recovering techniques from castrated dogs, tested in this study, can be used for semen refrigeration and cryopreservation.
DOI: 10.6084/m9.figshare.14280419
title: [Ponderal development and hematological and biochemical parameters of Pantaneiro lambs subjected to different suckling regimens]

description: ABSTRACT This study aimed to evaluate the weight development and the dynamics of hematological and biochemical parameters of Pantaneiro lambs submitted to different breastfeeding management from 15 to 43 days. First, 30 lambs were separated into three different groups (n = 10). The groups were characterized according to the length of stay of ewes with their young in different breastfeeding systems: MAM24 - ewes and lambs 24 hours together. MAM12- Sheep and lambs 12 hours together at night. MAM2x30 - sheep and lambs 30 minutes in the morning and 30 minutes in the afternoon together. Blood samples were collected and weighed every seven days. Significant increase in weight was observed with the age of the lambs in the three managements, but the treatments did not differ for weight gain. Differences were identified between the three treatments for the hematological variable CHGM and for the biochemical variables AST, glucose, urea and total protein. Correlating the age of the lambs with the biochemical variables, there was variation influenced by the age of the animals. The different management of breastfeeding did not influence the weight development of the lambs. Hematological and biochemical variations did not represent pathological changes. Some hematological and biochemical parameters may be influenced by the age development of lambs.
DOI: 10.6084/m9.figshare.14280414.v1
title: [A critical-historical analysis of the continuous development of Brazilian legislation related to artisanal cheeses]

description: ABSTRACT Different artisanal cheeses are produced, commercialized and consumed in Brazil, leading to a constant development of related rules by the MAPA and other official agencies. The establishment of two national programs (SUASA and SISBI-POA) allowed an equivalence in inspection system and an expanded distribution. Also, MAPA allowed ripening time lower than 60 days for artisanal raw milk cheeses, based on scientific studies that assure their safety. However, lowering the ripening period is still controversial, once there are no proper established criteria for such scientific studies, leading to potential multiple interpretation of data. The newly established ARTE certification transferred the inspection responsibilities of artisanal products to secretaries of agriculture, livestock and health, in support of what was already predicated by MAPA and SISBI-POA. Based on ARTE certification, the inspection service must also provide orientation guidance to producers, which should be done specifically by extension organs and associations. The norms that guide the production and commercialization of these artisanal products often need to be updated, but based on well-established methodologies and procedures, to ensure the distribution of suitable products to consumers.
DOI: 10.6084/m9.figshare.14280416
title: [Dispersion of lidocaine epidurally administered to dogs positioned on lateral or external decubitus]

description: ABSTRACT The aim of this study was to clinically verify the dispersion of lidocaine in the epidural space of dogs placed in different positions. Sixteen dogs with an average weight of 17.5 kilograms were used. These were tranquilized with acepromazine, anesthetized with propofol and allocated to two groups: sternal decubitus (GE) and right lateral decubitus (GLD). Both groups received 2% of lidocaine in the volume of 0.25mL/kg and remained in the same position for 20 minutes. The blocking of the pelvic limbs and the extension of it from the seventh lumbar vertebra were evaluated by means of interdigital and paravertebral panniculus clamping. Orchiectomy surgery was then performed. Afterwards, the total blocking time of the pelvic limbs was evaluated. All dogs presented bilateral blocking, with no differences in cranial extension between groups, with a median of 7.5 (1-14) vertebrae for GE and 4 (1-14) for GLD. The blocking time of the right and left limbs were 123 ± 26 and 130 ± 20 minutes for GE, and 120 ± 21 and 121 ± 20 minutes for GLD with no difference between groups or between limbs. It is concluded that the decubitus does not interfere with the epidural lidocaine distribution.
DOI: 10.6084/m9.figshare.14280418
title: Supplementation of lamb ewes with different protein sources in deferred marandu palisadegrass (Brachiaria brizantha cv. marandu) pasture

description: ABSTRACT The objective of this study was to evaluate the effect of different protein sources on the performance of lamb ewes and their offspring supplemented in deferred pasture. The treatments were the different protein sources: soybean meal, cotton cake, babassu cake and coconut cake. The ewes were weighed and evaluated for their body condition score during the gestation, parturition and weaning phases. The lambs were weighed at birth and at weaning. The structural variables and chemical composition of the pasture were evaluated during the usage period. Grass height and forage mass were higher when the ewes were in the gestation and weaning stages. The leaf mass was not altered during the pasture usage with a mean value of 566.1kg ha-1 DM. The highest levels of crude protein in the leaf blade were observed during the parturition and weaning phases. The body weight of the ewes did not differ between the sources with averages 45.0, 39.9 and 36.6kg for the gestation, parturition and weaning phases, respectively. Weights at birth and at weaning had an effect from protein sources, with the highest values observed in the lambs of ewes who received soybean meal and babassu cake supplementation. The lambs of ewes supplemented with soybean had higher average daily gain and total gain. Babassu, coconut and cotton cakes can replace soybean meal in supplementation of ewes in deferred pasture.
DOI: 10.6084/m9.figshare.14280422
title: Glutamine use in feeding juvenile pirarucu, Arapaima gigas (Schinz, 1822)

description: ABSTRACT This study aimed to evaluate glutamine supplementation effects on variables of growth performance, body composition, intestinal morphology and enzymatic aspects of juvenile Arapaima gigas. Research was conducted at the Fish Nutrition and Feeding Laboratory, where 60 examples of pirarucu (initial average weight of 82.12g) were distributed over 15 polyethylene tanks (310L), in a completely randomized design, with five treatments and three repetitions (four fish per experimental unit). Experimental diets were prepared containing five inclusion levels of the amino acid glutamine (0.0, 0.5, 1.0, 1.5 and 2.0%), supplied three times a day for 45 days. Quadratic effect was observed for the variables of growth performance, weight gain, food consumption, food conversion, and specific growth and protein efficiency rates. A significant effect was observed on intestinal villi at the height of the anterior portion and on activity of the enzyme’s alkaline proteases, lipase, amylase and aspartate aminotransferase. However, glutamine supplementation had no significant effect on survival rate. Inclusion of 1.02% of glutamine in the diets of juvenile pirarucu improved growth performance and influenced intestinal villi height and activity of important digestive enzymes, favoring nutrient digestion and absorption.
DOI: 10.6084/m9.figshare.14280418.v1
title: Supplementation of lamb ewes with different protein sources in deferred marandu palisadegrass (Brachiaria brizantha cv. marandu) pasture

description: ABSTRACT The objective of this study was to evaluate the effect of different protein sources on the performance of lamb ewes and their offspring supplemented in deferred pasture. The treatments were the different protein sources: soybean meal, cotton cake, babassu cake and coconut cake. The ewes were weighed and evaluated for their body condition score during the gestation, parturition and weaning phases. The lambs were weighed at birth and at weaning. The structural variables and chemical composition of the pasture were evaluated during the usage period. Grass height and forage mass were higher when the ewes were in the gestation and weaning stages. The leaf mass was not altered during the pasture usage with a mean value of 566.1kg ha-1 DM. The highest levels of crude protein in the leaf blade were observed during the parturition and weaning phases. The body weight of the ewes did not differ between the sources with averages 45.0, 39.9 and 36.6kg for the gestation, parturition and weaning phases, respectively. Weights at birth and at weaning had an effect from protein sources, with the highest values observed in the lambs of ewes who received soybean meal and babassu cake supplementation. The lambs of ewes supplemented with soybean had higher average daily gain and total gain. Babassu, coconut and cotton cakes can replace soybean meal in supplementation of ewes in deferred pasture.
DOI: 10.6084/m9.figshare.14280422.v1
title: Glutamine use in feeding juvenile pirarucu, Arapaima gigas (Schinz, 1822)

description: ABSTRACT This study aimed to evaluate glutamine supplementation effects on variables of growth performance, body composition, intestinal morphology and enzymatic aspects of juvenile Arapaima gigas. Research was conducted at the Fish Nutrition and Feeding Laboratory, where 60 examples of pirarucu (initial average weight of 82.12g) were distributed over 15 polyethylene tanks (310L), in a completely randomized design, with five treatments and three repetitions (four fish per experimental unit). Experimental diets were prepared containing five inclusion levels of the amino acid glutamine (0.0, 0.5, 1.0, 1.5 and 2.0%), supplied three times a day for 45 days. Quadratic effect was observed for the variables of growth performance, weight gain, food consumption, food conversion, and specific growth and protein efficiency rates. A significant effect was observed on intestinal villi at the height of the anterior portion and on activity of the enzyme’s alkaline proteases, lipase, amylase and aspartate aminotransferase. However, glutamine supplementation had no significant effect on survival rate. Inclusion of 1.02% of glutamine in the diets of juvenile pirarucu improved growth performance and influenced intestinal villi height and activity of important digestive enzymes, favoring nutrient digestion and absorption.
DOI: 10.6084/m9.figshare.14280423
title: [Detoxification and nutritional profile of the detoxified castor by different alkaline solutions]

description: ABSTRACT This study aimed to evaluate the detoxification of crude castor (DCC) through two alkaline products in different concentrations and their effects on the chemical composition, in situ degradability of DM and the fractionation of proteins. We used the calcium hydroxide [Ca(OH)2] and sodium hydroxide (NaOH) in two concentrations (60 and 90 grams) diluted in 4 quantities of water (1,000; 1,500; 2,000 and 2,500ml of water per kilo of DCC). It was observed that in the different concentrations used, only the use of 90 and 60 grams of Ca(OH)2 and NaOH, respectively managed to detoxify 100% of the DCC, both diluted in 2,000ml of water. On the other hand, when assessing the minimum time of contact of the reagents with the DCC for maximum detoxification, it was observed that with three hours of contact is the time required for the reagents decrease in 100% of the cytotoxic proteins, in addition to not leave haemagglutinating activity in this material. The detoxification with NaOH provided greater degradation of soluble proteins and degradation of dry matter, favoring the provision of non-protein nitrogen, while its application on an industrial scale is in the dependence of studies on operational feasibility and cost.
DOI: 10.6084/m9.figshare.14280426
title: Reproductive traits in different Nile tilapia genetic groups

description: ABSTRACT The aim of this study was to evaluate the reproductive traits of the non-inbred and inbred AquaAmérica, GIFT and AquaAmérica × GIFTgenetic groups. Six fish from each genetic group were used (2 females:1 male). Females were examined for the presence of eggs in their mouth at every four days, for 12 weeks. Reproduction occurred in all genetic groups (GIFT: 100%; non-inbred AquaAmérica and AquaAmérica ×GIFT: 75%; inbred AquaAmérica: 50%). Female weight, female standard length, total spawning weight, absolute fecundity, relative fecundity, spawn index and hatching rate did not differ significantly between the genetic groups. However, the non-inbred AquaAmérica variety showed lower values (P&lt;0.05) for egg diameter (2.4mm) and egg weight (4.2mg) and higher values (P&lt;0.05) for relative number of eggs (247.6 eggs/g of egg) than GIFT (egg diameter: 2.8mm; egg weight: 5.7mg; relative number of eggs: 175.4 eggs/g of egg) and AquaAmérica ×GIFT (egg diameter: 2.8mm; egg weight: 5.9mg; relative number of eggs: 168.8 eggs/g of egg). In conclusion, the non-inbred AquaAmérica variety produces smaller, lighter eggs but a higher relative number of eggs than the GIFT variety and the AquaAmérica ×GIFT cross; and inbreeding negatively affects spawning rate.
DOI: 10.6084/m9.figshare.14280428
title: Genetic parameters for weight, morphometry and oviposition of Africanized honeybee queens using simulated data

description: ABSTRACT This study aimed to estimate genetic parameters for simulated data of body weight (BW), abdominal width (AW), abdominal length (AL), and oviposition. Simulation was performed based on real data collected at apiaries in the region of Campo das Vertentes, Minas Gerais, Brazil. Genetic evaluations were performed using single- and two-trait models and (co)variance components were estimated by the restricted maximum likelihood method. The heritability for BW, AW, AL and oviposition were 0.54, 0.47, 0.31 and 0.66, respectively. Positive genetic correlations of high magnitude were obtained between BW and AW (0.80), BW and oviposition (0.69), AW and oviposition (0.82), and AL and oviposition (0.96). The genetic correlations between BW and AL (0.11) and between AW and AL (0.26) were considered moderate and low. In contrast, the phenotypic correlations were positive and high between BW and AW (0.97), BW and AL (0.96), and AW and AL (0.98). Phenotypic correlations of low magnitude and close to zero were obtained for oviposition with AL (0.02), AW (-0.02), and BW (-0.03). New studies involving these characteristics should be conducted on populations with biological data in order to evaluate the impact of selection on traits of economic interest.
DOI: 10.6084/m9.figshare.14280419.v1
title: [Ponderal development and hematological and biochemical parameters of Pantaneiro lambs subjected to different suckling regimens]

description: ABSTRACT This study aimed to evaluate the weight development and the dynamics of hematological and biochemical parameters of Pantaneiro lambs submitted to different breastfeeding management from 15 to 43 days. First, 30 lambs were separated into three different groups (n = 10). The groups were characterized according to the length of stay of ewes with their young in different breastfeeding systems: MAM24 - ewes and lambs 24 hours together. MAM12- Sheep and lambs 12 hours together at night. MAM2x30 - sheep and lambs 30 minutes in the morning and 30 minutes in the afternoon together. Blood samples were collected and weighed every seven days. Significant increase in weight was observed with the age of the lambs in the three managements, but the treatments did not differ for weight gain. Differences were identified between the three treatments for the hematological variable CHGM and for the biochemical variables AST, glucose, urea and total protein. Correlating the age of the lambs with the biochemical variables, there was variation influenced by the age of the animals. The different management of breastfeeding did not influence the weight development of the lambs. Hematological and biochemical variations did not represent pathological changes. Some hematological and biochemical parameters may be influenced by the age development of lambs.