cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/43g82-enc38
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC065
description: <strong>Tilt Series Date:</strong> 2014-05-12</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2014-05-12-2_slicer7303.jpg: external vesicle ycw2014-05-12-2_slicer7304.jpg: Type VI secretion system
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-2/3dimage_42382/</p>20140512_YWC065_20002_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-2/3dimage_42382/20140512_YWC065_20002_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20140512_YWC065_20002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-2/rawdata/20140512_YWC065_20002.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w8sk1-sqd03
title: Hyphomonas "SW57"

description: ParB-YFP, stalk, chromosome segregation, chromosome origin
description: <strong>Tilt Series Date:</strong> 2012-02-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in Marine Broth (MB) medium at 30 degrees. 1:20 diluted into GAMS-P medium and incubated for 48 hr at 30 degrees. 10 ml cells were pelleted and resuspended into 10 ml MB, incubate for 5 hr at 30 degrees. Further concentration was made by pelleted down whole culture solution and resuspended into 50 ul fresh MB.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nM BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-02-25-11/rawdata/</p>20120225_20004.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-02-25-11/rawdata/20120225_20004.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120225_20004_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-02-25-11/3dimage_17198/20120225_20004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7mbcm-mrs62
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-04-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-04-15-14_slicer7459.jpg: T6SS in Myxococcus xanthus PilO-sfGFP ycw2014-04-15-14_slicer7460.jpg: unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-14/rawdata/</p>20140415_YWC092_10014.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-14/rawdata/20140415_YWC092_10014.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140415_YWC092_10014_part131_3.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-14/3dimage_41654/20140415_YWC092_10014_part131_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fmdjr-xta05
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-03-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-10-26_slicer8392.jpg: Myxococcus Xanthus  .  filament ycw2014-03-10-26_slicer8393.jpg: Myxococcus Xanthus  .  extracellular vesicle .  pili
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-26/rawdata/</p>20140310_YWC092_10026.mrc,  Tilt Series (Pixel Size 3.9 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-26/rawdata/20140310_YWC092_10026.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140310_YWC092_10026_part131_40.rec,  Reconstruction (Pixel Size 15.6 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-26/3dimage_39954/20140310_YWC092_10026_part131_40.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ze2qm-c5k66
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-25-86_slicer10219.jpg: Helicobacter pylori .  flagellar motor .  chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-86/rawdata/</p>20131025_Janelia_YWC043_20032.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-86/rawdata/20131025_Janelia_YWC043_20032.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_20032_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-86/3dimage_33561/20131025_Janelia_YWC043_20032_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v14v1-z9125
title: magnetosome induced t1

description: Raw data files of magnetosome induced t1
description: <strong>Tilt Series Date:</strong> 2014-06-19</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum </p>
<strong>Strain:</strong> deltaQ 167</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
  psa2014-06-19-9_slicer4032.jpg: magnetosomes psa2014-06-19-9_slicer4033.jpg: magnetosomes t1 connected to membrane??? psa2014-06-19-9_slicer4034.jpg: magnetosomes t1 mamk with alligned vescicle???? psa2014-06-19-9_slicer4035.jpg: magnetosomes t1 in the cytoplasm?? psa2014-06-19-9_slicer4036.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4037.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4038.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4039.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4040.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4041.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4042.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4043.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer4044.jpg: magnetosomes t1 vescicle?? psa2014-06-19-9_slicer6692.jpg: unknown psa2014-06-19-9_slicer6693.jpg: peptidoglycan psa2014-06-19-9_slicer6694.jpg: filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-06-19-9/rawdata/</p>atlas40005.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-06-19-9/rawdata/atlas40005.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas40005_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-06-19-9/3dimage_43759/atlas40005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wr6te-6dq76
title: Myxococcus xanthus DK1622 wt

description: For T4P averaging
description: <strong>Tilt Series Date:</strong> 2013-08-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-08-26-13_slicer7649.jpg: pili in Myxococcus xanthus ycw2013-08-26-13_slicer7653.jpg: T6SS in Myxococcus xanthus, 20.379 nm
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-13/rawdata/</p>20130826_DK1622_WT_10013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-13/rawdata/20130826_DK1622_WT_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130826_DK1622_WT_10013_part131_4.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-13/3dimage_30130/20130826_DK1622_WT_10013_part131_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kdmcm-mcq35
title: Sulfolobus solfac.P2+STIV - 41KX

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 10 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-02-07</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-07-12/rawdata/</p>atlas8stiv100009.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-07-12/rawdata/atlas8stiv100009.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas8stiv100009_part111_108.rec,  Reconstruction (Pixel Size 1.04 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-07-12/3dimage_38526/atlas8stiv100009_part111_108.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qqzpk-x7n74
title: Vibrio cholerae MKW1383

description: In the mixture with Bdellovibrio.Something wrong with the dosage setting.
description: <strong>Tilt Series Date:</strong> 2013-09-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with overnight Vibrio cholarea MKW1383 for 90 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-10-20/rawdata/</p>20130909_Janelia_Bdellovibrio_Vibrio_10020.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-10-20/rawdata/20130909_Janelia_Bdellovibrio_Vibrio_10020.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130909_Janelia_Bdellovibrio_Vibrio_10020_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-10-20/3dimage_30756/20130909_Janelia_Bdellovibrio_Vibrio_10020_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dcv1f-3k020
title: Vibrio cholerae MKW1383

description: In the mixture with Bdellovibrio for 4 hr at 30C. Cells seemed lost PG layer without Bdellovibrio inside.
description: <strong>Tilt Series Date:</strong> 2013-10-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 4 hr at 30 C before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-43/rawdata/</p>montage_9x90016.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-43/rawdata/montage_9x90016.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_9x90016_part131_1.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-43/3dimage_32545/montage_9x90016_part131_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/k4p78-egy74
title: H. pylori 26695 / Hs.746T

description: Grid box YWC057
description: <strong>Tilt Series Date:</strong> 2013-12-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-17/rawdata/</p>20131216_YWC057_10011.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.5 GB  
        
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        20131216_YWC057_10011_part87_2.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
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