cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0rj4h2d
title: CIL:46077, Homo sapiens. In Cell Image Library

description: Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities.

The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.
description: Plate 26685 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.
 
Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).
 
This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.
DOI: 10.6075/j0nc5zsx
title: CIL:46073, Homo sapiens. In Cell Image Library

description: Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities.

The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.
description: Plate 26681 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.
 
Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).
 
This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.
DOI: 10.6075/j0cj8c2v
title: CIL:46070, Homo sapiens. In Cell Image Library

description: Plate 26678 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.
 
Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).
 
This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.
description: Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities.

The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.
DOI: 10.6075/j06q1w31
title: CIL:42752, Mus musculus, neuron. In Cell Image Library

description: Confocal image of an axonal rainbow of oculomotor nerve motor axons from a "Brainbow" mouse brain, with each neuron expressing a distinct color. In Brainbow mice, neurons randomly choose combinations of red, yellow and cyan fluorescent proteins, so that they each glow a particular color. This provides a way to distinguish neighboring neurons and visualize brain circuits. 2007 Olympus BioScapes Digital Imaging Competition.
For additional details see: Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW.
Nature. 2007 Nov 1;450(7166):56-62.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Yellow fluorescent proteins from Aequorea; Cyan fluorescent proteins from Aequorea; Red fluorescent proteins from Anthozoa
DOI: 10.6075/j09g5km2
title: CIL:42751, Mus musculus, axon. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Yellow fluorescent proteins from Aequorea; Cyan fluorescent proteins from Aequorea; Red fluorescent proteins from Anthozoa
description: Confocal image of "Brainbow" mouse cerebral cortex. 

In Brainbow mice, neurons randomly choose combinations of red, yellow and cyan fluorescent proteins, so that they each glow a particular color. This provides a way to distinguish neighboring neurons and visualize brain circuits. Honorable Mention, 2007 Olympus BioScapes Digital Imaging Competition.
 
For additional details see: Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW. Nature. 2007 Nov 1;450(7166):56-62.
DOI: 10.6075/j0h9941q
title: CIL:42803, lung cancer. In Cell Image Library

description: Colorized scanning electron micrograph of a cell cultured lung cancer cell.This image is part of an image group, CIL 42801-42803, showing several colorized scanning electron micrographs of cell cultured lung cancer cells.
description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: colorized
description: B0008520 Lung cancer cell. Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://images.wellcome.ac.uk/indexplus/page/Prices.html
DOI: 10.25420/britishmuseum.30478874
title: Name of the straw used during firing of pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the straw used to cover the pots during firing, called “rơm”. The potter grow the straw in their rice fields.
DOI: 10.25420/britishmuseum.30478877
title: Name of the bark used to fire pottery in Quê An village (Vietnamese language)

description: Giang Ngọc Xanh and Võ Thị Sương say the name of the bark used as fuel during firing: “vỏ tràm”, where “vỏ” means bark and “tràm” refers to “cây keo lá tràm”, vietnamese name of Acacia auriculiformi.
DOI: 10.25420/britishmuseum.30478877.v1
title: Name of the bark used to fire pottery in Quê An village (Vietnamese language)

description: Giang Ngọc Xanh and Võ Thị Sương say the name of the bark used as fuel during firing: “vỏ tràm”, where “vỏ” means bark and “tràm” refers to “cây keo lá tràm”, vietnamese name of Acacia auriculiformi.
DOI: 10.6075/j03x85hh
title: CIL:42754, Mus musculus, neuron. In Cell Image Library

description: Confocal"Brainbow" mouse hippocampus and cerebral cortex neurons, with several fields montaged together. In Brainbow mice, neurons randomly choose combinations of red, yellow and cyan fluorescent proteins, so that they each glow a particular color. This provides a way to distinguish neighboring neurons and visualize brain circuits. Honorable Mention, 2007 Olympus BioScapes Digital Imaging Competition.
For additional details see: Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW.
Nature. 2007 Nov 1;450(7166):56-62.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Yellow fluorescent proteins from Aequorea; Cyan fluorescent proteins from Aequorea; Red fluorescent proteins from Anthozoa
Processing history: montage
DOI: 10.25420/britishmuseum.30478862.v1
title: Name of the action of shaping pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action of shaping pots, nặn, which means to knead, to model.
DOI: 10.25420/britishmuseum.30478865
title: Name of the action of kneading clay in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action of kneading clay, which she calls “nhồi đất”, ‘đất’ being the word for “clay”. Nhồi, however doesn’t have any related meaning.
DOI: 10.25420/britishmuseum.30478865.v1
title: Name of the action of kneading clay in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action of kneading clay, which she calls “nhồi đất”, ‘đất’ being the word for “clay”. Nhồi, however doesn’t have any related meaning.
DOI: 10.25420/britishmuseum.30478871
title: Name of the cooking pot in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of a kind of cooking pot, called “om”.
DOI: 10.25420/britishmuseum.30478871.v1
title: Name of the cooking pot in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of a kind of cooking pot, called “om”.
DOI: 10.25420/britishmuseum.30478874.v1
title: Name of the straw used during firing of pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the straw used to cover the pots during firing, called “rơm”. The potter grow the straw in their rice fields.
DOI: 10.25420/britishmuseum.30478853.v1
title: Name of the bamboo tool used to scrape pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the tool made of a slice of bamboo with both ends tied together, called cái “khót”, where “cái” is the classifier for objects. It is used to scrape pottery, both when preforming on wet clay and when preforming on leather hard clay. The use of scrapers makes the edge blunt. Those that are too blunt to be used during preforming are used during smoothing.
DOI: 10.25420/britishmuseum.30478856
title: Name of the action of smoothing the outside of pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action of smoothing the outside of the pot after it has been scraped. This action is called “lặng”. The potter moistens the surface of the pots by hand. She then uses an old circle of bamboo with blunt edges to rub the walls of the pot to smooth the surface. The water re-moistens the walls. The clay particles on the surface become mobile again and settle into the small cavity created by the scraping.
DOI: 10.25420/britishmuseum.30478856.v1
title: Name of the action of smoothing the outside of pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action of smoothing the outside of the pot after it has been scraped. This action is called “lặng”. The potter moistens the surface of the pots by hand. She then uses an old circle of bamboo with blunt edges to rub the walls of the pot to smooth the surface. The water re-moistens the walls. The clay particles on the surface become mobile again and settle into the small cavity created by the scraping.
DOI: 10.25420/britishmuseum.30478859
title: Name of the structure to fire pot in Quê An village

description: Võ Thị Sương says the name of the firing structure in Vietnamese: lò gốm (lit. furnace pottery). In this village, the structure a simple circular structure, about 1m50 high and 45cm wide, made mostly of brick and clay. It has two openings. In the centre there is a raised circular platform 1m10 wide. Metal bars connect the walls and the top of the platform to let in the heat of the fire.
DOI: 10.25420/britishmuseum.30478859.v1
title: Name of the structure to fire pot in Quê An village

description: Võ Thị Sương says the name of the firing structure in Vietnamese: lò gốm (lit. furnace pottery). In this village, the structure a simple circular structure, about 1m50 high and 45cm wide, made mostly of brick and clay. It has two openings. In the centre there is a raised circular platform 1m10 wide. Metal bars connect the walls and the top of the platform to let in the heat of the fire.
DOI: 10.25420/britishmuseum.30478862
title: Name of the action of shaping pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action of shaping pots, nặn, which means to knead, to model.
DOI: 10.6075/j03j3btx
title: CIL:42257, Drosophila melanogaster, muscle cell, neuron. In Cell Image Library

description: Confocal micrograph showing the innervation of the posterior portion of the digestive tract of an adult Drosophila fly. The circular visceral muscles are labelled in red with phalloidin staining of actin. The main nerves are stained in green (with 22c10, a MAP1B antibody), and a subset of gut-innervating insulin-producing neurons is highlighted in green.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
DOI: 10.25420/britishmuseum.30478844
title: Name of the string used to cut the pots off the turntable in Quê An village

description: Võ Thị Sương says the name of the wire attached to two small sticks, cái dây. She uses this tool to cut off the lids of the turntable.
DOI: 10.25420/britishmuseum.30478847
title: Name of the sieve in Vietnamese language (Quê An village)

description: Giang Ngọc Xanh says the name of the traditional bamboo fibre sieve called cái giần. It was used in the past to sieve the ground clay. It has been nowadays replaced by a machine. However, it is still used to sieve the sand that is added to the clay for handles.
DOI: 10.25420/britishmuseum.30478847.v1
title: Name of the sieve in Vietnamese language (Quê An village)

description: Giang Ngọc Xanh says the name of the traditional bamboo fibre sieve called cái giần. It was used in the past to sieve the ground clay. It has been nowadays replaced by a machine. However, it is still used to sieve the sand that is added to the clay for handles.
DOI: 10.25420/britishmuseum.30478850
title: Name of the clay in Vietnamese language (Quê An village)

description: Võ Thị Sương says the name of the clay in Vietnamese. It is called “đất”
DOI: 10.25420/britishmuseum.30478850.v1
title: Name of the clay in Vietnamese language (Quê An village)

description: Võ Thị Sương says the name of the clay in Vietnamese. It is called “đất”
DOI: 10.25420/britishmuseum.30478853
title: Name of the bamboo tool used to scrape pottery in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the tool made of a slice of bamboo with both ends tied together, called cái “khót”, where “cái” is the classifier for objects. It is used to scrape pottery, both when preforming on wet clay and when preforming on leather hard clay. The use of scrapers makes the edge blunt. Those that are too blunt to be used during preforming are used during smoothing.
DOI: 10.25420/britishmuseum.30478838
title: Name of the action to fire pots in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action to fire pots. This process is called "chín" in Vietnamese, which means “ripe, cooked”. The potter light the fire on both sides and let it burn for about 3 hours. Throughout the firing process, they take turns watching and loading the fire.
DOI: 10.25420/britishmuseum.30478841
title: Name of the action to pre-fire pots in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action to pre-fire pots. This process is called cứng, which means 'hard, tough, rigid’. The aim of this step is to dry the pots completely and make them 'hard' so that they will not break during the firing process. The firing lasts for 30 minutes until the pots get black strains.
DOI: 10.25420/britishmuseum.30478841.v1
title: Name of the action to pre-fire pots in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action to pre-fire pots. This process is called cứng, which means 'hard, tough, rigid’. The aim of this step is to dry the pots completely and make them 'hard' so that they will not break during the firing process. The firing lasts for 30 minutes until the pots get black strains.
DOI: 10.25420/britishmuseum.30478844.v1
title: Name of the string used to cut the pots off the turntable in Quê An village

description: Võ Thị Sương says the name of the wire attached to two small sticks, cái dây. She uses this tool to cut off the lids of the turntable.
DOI: 10.6075/j0125rf8
title: CIL:42256, Drosophila melanogaster, muscle cell, neuron. In Cell Image Library

description: Confocal micrograph of the posterior portion of the digestive tract of an adult Drosophila fly. The circular visceral muscles are labelled in red with phalloidin staining of actin. The main nerves are stained in blue (with 22c10, a MAP1B antibody), and an insulin-like peptide (Ilp7), released by a subset of gut-innervating neurons, is labelled in green.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; phalloidin
DOI: 10.25420/britishmuseum.30478832
title: Name of the turntable used to make pot in Quê An village

description: Võ Thị Sương says the name of the turntable that she uses to make pottery. She calls it “cái chuay”, althout the word “chuay” has no related meaning (cái being the classifier for objects).
DOI: 10.25420/britishmuseum.30478832.v1
title: Name of the turntable used to make pot in Quê An village

description: Võ Thị Sương says the name of the turntable that she uses to make pottery. She calls it “cái chuay”, althout the word “chuay” has no related meaning (cái being the classifier for objects).
DOI: 10.25420/britishmuseum.30478835
title: Name of the action to scrape the inside of the pot in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action to scrape the inside of the pot using a ring of bamboo. This time the aim is to thin the walls. A lot of clay is removed in the process. She calls this action "cạo", which translates as "to shave" or "to scrape".
DOI: 10.25420/britishmuseum.30478835.v1
title: Name of the action to scrape the inside of the pot in Vietnamese (Quê An village)

description: Võ Thị Sương says the name of the action to scrape the inside of the pot using a ring of bamboo. This time the aim is to thin the walls. A lot of clay is removed in the process. She calls this action "cạo", which translates as "to shave" or "to scrape".