cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j01j98cx
title: CIL:37148, Cavia porcellus, pancreatic cell. In Cell Image Library

description: Transmission electron micrograph of ribonucleic (RNP) particles with EDTA from a cell fractionation from a guinea pig pancreas. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: cell fractionation
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x42,000.
DOI: 10.6075/j0zp44qm
title: CIL:37194, Cavia porcellus. In Cell Image Library

description: Preparation: osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
description: References:
Palade, G.E. 1952. J. Exp. Med. 95:285-298.
Palade, G.E. 1952. Anat. Rec. 114:427-451.
Sabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58. 
Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X25,000.
description: Transmission electron micrograph of a mitochondria and a lipid droplet from a guinea pig pancreas. The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0h41q84
title: CIL:37288, Actinobacillus pleuropneumoniae, bacteria. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi.

Related citation: White, D.C., G. Leidy, J.D. Jamieson and R.E.Shope. P, J. Exp. Med. 120:1-12, 1964.
description: Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
description: Transmission electron micrograph of bacteria hemophilus pleuropneumoniae. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0x065qk
title: CIL:37146, Cavia porcellus, pancreatic cell. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x10,400.
description: Transmission electron micrograph of exocytosis in a guinea pig pancreas. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j03b5xtn
title: CIL:37191, Rattus, hepatocyte. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X22,000.
description: Transmission electron micrograph of liver from a 1-day old rat showing mitochondria, rough endoplasmic reticulum and rosettes of glycogen granules. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.5281/zenodo.17132490
title: Dataset for: Three-dimensional nanoscale control of magnetism in crystalline Yttrium Iron Garnet

description: This repository contains the data supporting the findings reported in the article "Three-dimensional nanoscale control of magnetism in crystalline Yttrium Iron Garnet" by V. Levati, M. Vitali, et al.
DOI: 10.5281/zenodo.17493161
title: Dataset for: Three-dimensional nanoscale control of magnetism in crystalline Yttrium Iron Garnet

description: This repository contains the data supporting the findings reported in the article "Three-dimensional nanoscale control of magnetism in crystalline Yttrium Iron Garnet" by V. Levati, M. Vitali, et al.
DOI: 10.6075/j01r6pwj
title: CIL:41717, Cricetulus griseus, ovary. In Cell Image Library

description: High Voltage (1MeV) EM image of metaphase chromosomes isolated from CHO cells using a non-aqueous technique, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 55 degrees. The accompanying image has a tilt of 45 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.
description: Cells were metaphase arrested with thymidine followed by colcemid, then exposed to a low ionic strength swelling medium (0.07M phosphate buffer). Chromosomes were isolated by the non-aqueous hexylene glycol technique of Wray and Stubblefield, squashed in 1:1 acetic acid:ethanol,stained, and transferred to an EM grid.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: Preparation: chemically fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j09p308v
title: CIL:37263, Bos taurus. In Cell Image Library

description: Preparation: osmium tetroxide fixed tissue; n-butyl methacrylate embedded tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: lead salt
Processing history: scanned negative
description: Zymogen granule fraction Calf. 
 Zymogen granules show their homogeneous content and limiting membrane. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x17,500.
DOI: 10.6075/j0k64hdw
title: CIL:41454, Cricetulus griseus, epithelial cell. In Cell Image Library

description: High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section through a Chinese hamster ovary cell at metaphase showing a chromatid pair, with kinetochores at the centromere. The image was taken with a specimen tilt of 62 degrees and is one of a group of 10 tilts from 77 degrees to 32 degrees at 5 degree intervals. Pairs of images provide oblique stereo views of the chromosome.
description: CHO cells at metaphase were fixed with paraformaldehyde followed by OsO4, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranly acetate for HVEM observation.
See also:
H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96
H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0891582
title: CIL:41727, Cricetulus griseus, ovary. In Cell Image Library

description: High Voltage EM image of CHO metaphase chromosomes from a 'thick' section of metaphase-arrested cells treated with D20 to stabilize microtubules. This image was recorded with a specimen tilt of 45 degrees. The accompanying grouped image had a tilt of 55 degrees to create a stereo pair providing an oblique 3D view of the chromosomes.
description: Preparation: chemically fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation at 1 MeV. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
DOI: 10.6075/j0d21x1v
title: CIL:41714, Cricetulus griseus, ovary. In Cell Image Library

description: High Voltage (1MeV) EM image of metaphase arrested CHO cell isolated using a non-aqueous technique, spread on a buffer surface and transferred to an EM grid. A single chromatid pair is seen in the center of the image. This micrograph was captured at a specimen tilt of 50 degrees. The accompanying image has a tilt of 60 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.
description: Preparation: chemically fixed tissue
Relation to intact cell: squash
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Cells were metaphase arrested with colcemid, then exposed to a low ionic strength medium (0.07M phosphate buffer). Chromosomes were prepared by a non-aqueous technique, spread on the surface of a low ionic strength buffer, transferred to an EM grid for examination in the HVEM.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
DOI: 10.6075/j0bc3xxr
title: CIL:41603, Cricetulus griseus, epithelial cell, ovary. In Cell Image Library

description: CHO cells arrested at metaphase with colcemid were treated with 67% D2O, in 0.07M phosphate buffer and squashed in 50% acetic acid. Preparations were transferred to EM grids and examined with the Wisconsin HVEM operated at 1MeV.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: High Voltage Transmission EM of whole mitotic metaphase chromosomes from Chinese hamster ovary cells. This image was recorded at a specimen tilt of 48 degrees and is grouped with one taken at 58 degrees, creating a stereo pair giving an oblique 3D view of the chromosomes.
description: Preparation: chemically fixed tissue
Relation to intact cell: squash preparation
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j05h7f6w
title: CIL:35278, Rattus rattus, hepatocyte. In Cell Image Library

description: Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus.
 
 This is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists.
description: Image was collected with 1 60X, 63X, or 100X oil immersion objective with a numerical aperture of 1.25 such that each pixel size was 0.23 X 0.23 um. The image was collected as part of a Z series with step size of 0.53 um. A Photometrics cooled CCD camera based on the KAF1400 chip was used.
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Rhodamine; primary antibody plus labeled secondary antibody
Processing history: 3D-deconvolution
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0416vq2
title: CIL:37241, fibroblast. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. The original magnification was 1,000X.
description: Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
description: This phase contrast image of a fibroblast was made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0cc0zbq
title: CIL:37167, Rattus, goblet cell. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x33,600.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Scanning electron micrograph of rat colon mucosa goblet cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0zg6sb7
title: CIL:37154, Rattus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: time lapse microscopy
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Confocal images were collected on an Olympus Fluoview 1000 microscope (IX81 base) equipped with a 60X/1.35 NA (oil)UPLSAPO 60X objective (Olympus). DsRed was excited with the 543 nm laser line of a He-Ne laser. Fluorescence emission was collected using the following dichroic mirror/filter: SDM640/BA560–620.
description: This is Video S1 and it corresponds to Figure 2A.
 It shows that local blebbistatin micropipetting, but not DMSO, increases spine formation and extension (arrowheads). Equal volumes of blebbistatin and DMSO were used. Images were acquired every ~6 seconds using confocal microscopy. 20 frames/sec shown.
 
 A DIV 7 cortical neuron expressing DsRed2 was locally micropipetted with either DMSO or 100 mM blebbistatin at the indicated times. Note the increase in the fraction of spines that appear and extend in response to blebbistatin. Arrowheads indicate either nascent or elongating spines.
DOI: 10.6075/j0qv3kwd
title: CIL:41709, Cricetulus griseus, ovary. In Cell Image Library

description: Cells were metaphase arrested with thymidine followed by colcemid, then exposed to a low ionic strength swelling medium (0.07M phosphate bufferin 50% D2O). Chromosomes were isolated by the non-aqueous hexylene glycol technique of Wray and Stubblefield, squashed in 1:1 acetic acid:ethanol,stained, and transferred to an EM grid.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: Preparation: chemically fixed tissue
Relation to intact cell: squash
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: High Voltage (1MeV) EM image of a metaphase chromosome isolated from CHO cells using a non-aqueous technique, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 45 degrees. The accompanying image has a tilt of 55 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.
DOI: 10.6075/j00k27m1
title: CIL:9062, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: The events of meiosis I in a living insect spermatocyte beginning at metaphase with meiotic bivalents at the spindle equator followed by anaphase , segregation of bivalents to the spindle poles and the near completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 90 min by a QImaging Retiga EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
DOI: 10.6075/j0v69hmz
title: CIL:9154, Homo sapiens, permanent cell line cell, epithelial cell. In Cell Image Library

description: Preparation: unfixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: maximum projection
description: Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). 
Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points.

BFA was added according to the following protocol:
Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart)
Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart) 
Timepoint3: 238 seconds (11 Z-slices at 1 um apart) Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point) 

Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.
DOI: 10.6075/j000014n
title: CIL:9153, Rattus, Purkinje cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; cryofixed tissue; tissue in plastic embedment; microtome-sectioned tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); immuno-electron microscopy
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt
Processing history: photoshop enhancement of gold
Data qualification: Processed;spatialmeasurements
description: EM micrograph demonstrating the colocalization of GABA neurotransmitter (30 nm gold) and delta 1/2 glutamate receptors (10 nm gold) in the cerebellar cortex molecular layer at P10. It shows Delta labeling (arrowheads) is abundant at the postsynaptic membrane of parallel fiber (pf) /Purkinje cell spine synapses. These postsynaptic spines are filled with GABA neurotransmitter (Purkinje neurons are GABAergic). Scale bar is 500 nm. Gold particles have been highlighted using Adobe Photoshop. The figure is modified from figure 4a in Zhao et al., J. Neurosci. 18:5517-28, 1998.
DOI: 10.6075/j0p849xr
title: CIL:9151, Rattus, interneuron. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: primary antibody plus labeled secondary antibody; gold
Processing history: unprocessed raw data
description: Transmission electron micrograph using immuno-gold labeling to show the location of PSD-95 in 3 glutamatergic synapses on an interneuron dendrite from the CA1 stratum radiatum of the hippocampus of an adult male rat (postnatal day 37). PSD-95 is labeled with a new rabbit monoclonal antibody (Cell Signaling Technology, Inc. - cat. #3450) and 10 nm immunogold (Ted Pella, GFAR). pre=presynaptic terminal, mit=mitochondrion, scale bar is 100 nm
DOI: 10.6075/j08k784j
title: CIL:9067, Nephrotoma suturalis, spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
description: The events of meiosis I in a living insect spermatocyte beginning at metaphase I with bivalents at the spindle equator followed by anaphase, segregation of half-bivalents diakinesis and commencement of cytokinesis. Time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 105 min by a QImaging Retigo EXi CCD camera. Raw images were processed using 4-frame algorithm (Shribak and Oldenbourg, 2003).A movie prepared from this image stack is included in this grouped set.