DOI: 10.5281/zenodo.10102338
title: Neuro.OralCard Database

description: INTRODUCTION: This study focuses (Martins, 2021) on a database literature review (Sousa, Martins et al., 2016) of the neuronal proteome found both in neurotypical subjects and in the ICD-11 chapters 'Diseases of the Nervous System' and "Mental and Behavior Disorders", the Neuro.OralCard. This database offers an update of the OralCard (Arrais et al., 2013), revising 2416 neuronal proteins identified in neuroproteomics, from which 765 are present, detected, and identifiable in the saliva proteome. The saliva proteome database, published as the bioinformatics tool OralCard (Arrais et al., 2013), was obtained from SalivaTec, CIS, UCP (Rosa et al., 2012). This database, as a set of UniprotKB codes, was cross-mapped to the set of UniprotKB obtaining the Neuro.OralCard, the neuronal proteome database.
METHODOLOGY: A systematic bibliographic search was performed using the search tool at PubMed's portal, complemented by Google Scholar searching utilities (Falagas et al., 2008). The following keywords were used as filters: "MeSH (Medical Subject Headings)", "neurological diseases", "psychiatric diseases", and "salivary diagnosis." In order to perform an ordered review based on the publications on molecular biology and the nervous system, additional keyword correlations and associations were searched. Only the publications from 2000 to 2021 were selected. Likewise, in order to perform an ordered review based on the correlation between the salivary proteins/nervous system proteins and physiological and pathologic conditions of neuropsychiatric conditions, the following search filters were also applied: "Humans" and "Clinical trial" in order to include, exclusively, articles of an experimental nature concerning the species Homo sapiens. Based on the extensive analysis (Neuro.OralCard References (2020). https://tinyurl.com/Neuro-OralCardReferences) of the 79 filtered and compiled articles, a survey of a representative sample of neuronal and salivary proteins identified in subjects with (i) neuropsychiatric conditions and (ii) healthy mental functioning was carried out. With the results obtained from this analysis, it was possible to create a neuronal proteome database named Neuro.OralCard. The full database has not yet been deposited in the oral proteome databank of the OralCard (Arrais, 2013). This specific analysis enables the updating of the OralCard, which is allusive to the human Oralia in different pathologic states. Meanwhile, the full neuroproteome (UniprotKB) was annotated in a non-restricted access server in the LIMMIT Lab, Faculty of Medicine, University of Lisbon, and accessible via: https://www.limmit.org/uploads/2/6/8/4/26841837/neuro.oralcard.xlsx (retrieved August 01, 2023).
RESULTS: The final neuronal database, named Neuro.OralCard, revised not only neuronal produced but also peripheral proteins. These neuronal proteins ultimately aim to represent not only epigenome, transcriptome, proteome, and metabolome analysis but also the main findings of functional cellular assays. The results of this neurobiological approach (Martins et al., 2023) imply that alterations in neurotransmission, hormonal regulation, metabolism, the cell cycle, and the immune system may be partially responsible for neuronal and mental pathophysiology. The Neuro.OralCard database comprises 2416 proteins concerning ICD-10 neuropsychiatric conditions and mental health functioning.
CONCLUSION: The Neuro.OralCard database aims to be used as a molecular data reference and support for biomarker discovery in neuropsychiatric illnesses and nonpathologic conditions of the nervous system.