DOI: 10.5061/dryad.j3tx95xnr
title: Adhesive droplets made from plant-derived oils for control of western flower thrips

description: Arthropod pests cause significant problems in agricultural crops all
 around the world. As chemical pesticide use becomes less desired, there is
 a need for alternative methods of pest control. Inspired by the natural
 adhesiveness of arthropod trapping plants, we examined the effectiveness
 of adhesive droplets made from oxidised and cross-linked plant-derived
 oils for control of western flower thrips. Two filter paper droplet
 adhesiveness assays and three detached chrysanthemum leaf assays were
 carried out to test efficacy against thrips. Suspensions containing
 adhesive droplets and other constituents were applied to filter papers and
 leaves via spraying or dipping. On filter papers, droplets made from
 oxidised rice germ oil (RGO) of different sizes caught 40–93% of thrips.
 Droplets made of a mixture of sunflower, olive, and linseed oil (MIX)
 caught up to 94% of thrips. Likewise, adhesive droplet-treated filter
 papers showed higher thrips mortality than untreated or control
 solution-treated filter papers. On chrysanthemum leaves, thrips were
 caught by both RGO (up to 40%) and MIX droplets (up to 20%) and thrips
 damage and reproduction were reduced. On MIX-treated leaves, thrips
 mortality was also increased. Within treatments, droplets of different
 size classes occurred and larger droplets were more effective at catching
 thrips in general. Droplets were also robust to rinsing with water, which
 is of importance for their application in horticulture. In conclusion,
 adhesive droplets made from edible plant oils show potential for use in
 control of western flower thrips.
description: <em>Data type: </em>experimental data, various
 measurements of Insect-bioassays performed in Petri-dishes.
 <em>Aim:</em> to assess if adhesive droplets made
 from natural materials could trap thrips (tiny insects and a major
 pest) <em>Methodolgy:</em>
 Exp1: filter papers were sprayed with different sizes (R180,
 R360, R720) adhesive droplets made from oxidized rice germ oil. Thrips
 were added and at several timepoints the number of thrips stuck in the
 droplets was counted. Exp2: Same as experiment 1, but
 with 2 different droplet prototypes (Mix, made from a mixture of vegetable
 oils) (RGO360-90, same as RGO360, but temperature used during oxidation
 set at 90C) Exp3: same as experiment 1, but using
 detached chrysanthemum leaves that are placed in agar. only a single
 measurement after 5 days. damage to the leaf by thrips feeding was also
 measured, as well as reproduction of the thrips. Exp4:
 same type of detached leaf assay as Exp3, but using the samples Mix and
 RGO360-90), this time particle coverage was also measured. application of
 droplets via dipping. Exp5, same as Exp4, but this time
 droplets were sprayed on the leaf. Excel files with
 experimental data were obtained by manual labor. For
 details on the methods see the associated
 publication: https://doi.org/10.1007/s10340-024-01755-4
description: # Adhesive droplets made from plant-derived oils for control of western
 flower thrips
 [https://doi.org/10.5061/dryad.j3tx95xnr](https://doi.org/10.5061/dryad.j3tx95xnr) ## Description of the data and file structure This dataset belongs to a study on the effectiveness of adhesive droplets made from natural materials against western flower thrips (Frankliniella occidentalis) The following data is included: Excel files of all 5 bioassays performed in the study, containing various types of measurements. Images from experiments 1,2,4,5, which were used to determine droplet coverage in the study. There are 5 excel datasets. **Datasets:** *Dataset1: 20220308_TB_JPS_exp1_WUR_180360720_filterpaper_V5* Sheet1: General information Sheet2: Exp1 thriplength rearing RAW, contains measurements on the length in mm of 100 thrips from the thrips colony used in the experiments. Sheet3: Exp1 thriplength in particle, contains measurements of the droplets on filterpapers that caught thrips (area in mm2) and the number and length of thrips caught in them. This is a subset of the data in sheet4. Sheet4: Exp1 dropcount thriplength RAW, the complete dataset of all droplets on filter papers. Sheet5: Exp1 filterpaper assay RAW, this sheet contains various measurements of the bioassay, such as coverage with droplets, number of thrips stuck and alive per filter paper for each timepoint. Sheet6: Exp1filterpaper Longformat Spss, same data as sheet 5, but in long format for analysis in spss Sheet7: Fig1 and S1 calculation data, contains the data and calculations used to make figure 1 and S1 of the publication. The aim of this sheet was to calculate the effectiveness of the droplets relative to the total number of droplets and relative to their total area. Some spots in the dataset are empty, this is because no thrips was caught in that size (droplets are binned by size per 1mm2 area) of droplets Sheet8: Fig1 RegressionSpss data, same data as in the rightmost columns of sheet7, used for analysis in spss. *Dataset2: 20220308_TB_JPS_exp3_WUR_180360720_ChrysBaltica_leafassay_thrips* Sheet1: general information Sheet2: Exp3 detachedleaf RAW, contains data of the detached leaf assay with RGO (rice germ oil) droplets. There were three types of RGO-derived adhesive droplets tested, made using different grind settings (180, 360, 720). These droplets were applied to detached chrysanthemum leaves, which were placed in agar in a 90mm Petri-dish. Thrips were added to the Petri-dishes and the experiment was left to run for 5 days as described in the publication. The type of data is similar to Sheet5 of dataset1. Totaldamage is the total amount of thrips damage in mm2, L1 larvae are thrips larvae of the firs stage, which were be observed after 3-5 days at 25C. *Dataset3 20221213_TB_JPS_exp2_WUR_MIX_90_filterpaper_V6* Sheet1 General information Sheet2 Exp2 dropcount thriplength RAW, contains information about the particles sprayed on filter papers in experiment 2. 'mix' are the first type of particles, '90' are the second type of particles (named RGO 360-90 in the publication). Sheet3 Exp2 thripslength data, a subset of the data on sheet2. Sheet4 Exp2 filterpaper assay RAW, data on the second filterpaper assay. Sheet5 Fig1_S1 calculations graphs, contains the data and calculations used to make figures 1 and S1 of the publication. Similar type of data as in Sheet7 of dataset 1. Sheet6 Fig1 regression data, data of the rightmost columns of sheet 5, used for analysis in spss. *Dataset 4: 20230331_TB_JPS_exp5_WUR_detachedleaf_spray_MIX* Sheet1: General information Sheet2: Exp5 dropcount thriplength RAW, contains data on the area of droplets on detached leaves (as analyzed by imageJ) and the number of thrips caught in the droplets. Sheet3: Exp5_detachedleaf_MIX_spray_RAW, contains various measurements on the second leaf assay (adhesive droplets applied to leaf via spraying). Abaxial is the upside of the leaf, adaxial is the bottom side. The rightmost columns contain details on the area of the droplets that caught thrips. *Dataset 5: 20230503_TB_JPS_exp4_WUR_detachedleaf_dip_MIX* Sheet1: General information Sheet2: exp4 det_leaf_dip_droplets_RAW, similar type of data as Sheet2 of dataset 4. Sheet3: exp4_leafdip_MIX_sol_wat_RAW, contains data on various measurements of the Petri-dish detached leaf bioassay of leaves that had adhesive MIX (a mixture of different vegetable oil) droplets on them (applied via dipping). 'Solution' is a soltion of aglinic acid, pluronic F108, and CaCl2 (a gel-like fluid that helps to suspend the MIX adhesive droplets), here it serves as a control treatment for the MIX droplets. Data is similar to data of Sheet3 of dataset 4. Note: Some cells are intentionally left blank as they contain summary information, calculations, or are void of data because they are associated with control treatments where no measurements could be done on certain parameters, or only a few observations were made within a column for specific replicates. Within the context of the dataset we provide more clarity and explanations under the info tab on each sheet regarding the data, in the context of which it becomes clear what the missing values mean. **Images** *Images_Exp1_Petridish_WUR_180360720* *Images_Exp2_Petridish_WUR_RGO90_MIX* *Images_Exp4_leafassay_WUR_MIX3_dip* *Images_Exp5_leafassay_WUR_MIX3_spray* These four folders contain RAW images (as taken with the camera) and other images related to particle analysis. Each time the filter paper or leaf is cut out and the particles are selected using ImageJ (method as described in the supplement belonging to the paper). ## Sharing/Access information Data may also be obtained from Leiden University.