DOI: 10.22002/yav35-v7f41
title: Caulobacter crescentus

description: wild type CB15N. 3 of 4, not warped. pre-divisional cell, non-stalk end; S-layer intact; 2 amorphous blobs at ends of visible area of cell; short filament seen at non-stalk end + 2 fainter, less convincing filaments; 1 well-defined storage granule & 1 small fuzzy one; 'proto-blebs' in OM on either side of non-stalk end; possible crystalline density near main filament
description: <strong>Tilt Series Date:</strong> 2006-08-15</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; 7 days growth; see DPD notebook Aug 07-14, 2006</p>
<strong>Sample Preparation:</strong> box 705, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006</p>
  SAVE_cryst1_xy2x_01.jpg: file associated to 3D Image #323 SAVE_cryst1_yz2x_01.jpg: file associated to 3D Image #323 SAVE_fil1_xy2x_01.jpg: file associated to 3D Image #323 SAVE_fil1_xy2x_02.jpg: file associated to 3D Image #323 SAVE_fil2_xy2x_01.jpg: file associated to 3D Image #323 SAVE_fil2_xy2x_02.jpg: file associated to 3D Image #323 SAVE_fil3_xy2x_01.jpg: file associated to 3D Image #323 pdd2006-08-15-3_slicer10056.jpg: Caulobacter crescentus .  inclusion body? .  unknown density pdd2006-08-15-3_slicer10057.jpg: Caulobacter crescentus .  granule
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-08-15-3/rawdata/</p>CC06Aug15e150t03.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
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        06Aug15t03bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
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        SAVE_06Aug15t03_18kx..dm3,  file associated to 3D Image #323, 8.6 MB  
        
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        SAVE_cryst1_xy2x_01.tif,  file associated to 3D Image #323, 5.9 MB  
        
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        SAVE_cryst1_yz2x_01.tif,  file associated to 3D Image #323, 5.9 MB  
        
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        SAVE_fil1_xy2x_01.tif,  file associated to 3D Image #323, 5.9 MB  
        
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        SAVE_fil1_xy2x_02.tif,  file associated to 3D Image #323, 5.9 MB  
        
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        SAVE_fil2_xy2x_01.tif,  file associated to 3D Image #323, 5.6 MB  
        
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        SAVE_fil2_xy2x_02.tif,  file associated to 3D Image #323, 5.6 MB  
        
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        SAVE_fil3_xy2x_01.tif,  file associated to 3D Image #323, 5.6 MB  
        
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