DOI: 10.5061/dryad.fttdz0969
title: Data from: Ejaculate investment differs by population, but not wing morph or perceived sperm competition risk, in Pacific field crickets

description: We raised crickets from two populations in Hawaii (O'ahu and
 Kaua'i) and two wing morphs (flatwing males, which cannot sing; and
 normal-wing males, which can sing), under conditions of perceived sperm
 competition risk (presence of male advertisement song) or its absence
 (quiet). In adulthood, males were euthanized, frozen, measured for body
 size and mass; their accessory glands (which produce seminal fluid) were
 dissected out and preserved for quantitative PCR (qPCR) analysis. We
 examined the effects of population, wing morph, and presence or absence of
 perceived sperm competition risk on male testes mass and production of
 three seminal fluid proteins (SFPs) implicated in male paternity success.
 The dataset includes a spreadsheet reporting data, including: male
 population of origin, wing morph, experimental acoustic treatment (song
 present or absent), body mass, pronotum width, and qPCR values for each of
 the three SFPs. It also includes R code used to analyze the data and
 generate the figures. Finally, it includes acoustic files and MATLAB code
 that were used to generate the stimulus we used to experimentally induce
 the perceived threat of sperm competition.
description: # Data from: Ejaculate investment differs by population, but not wing
 morph or perceived sperm competition risk, in Pacific field crickets
 Dataset DOI:
 [10.5061/dryad.fttdz0969](https://doi.org/10.5061/dryad.fttdz0969) ##
 Description of the data and file structure We studied seminal fluid
 expression in Pacific field crickets (*Teleogryllus oceanicus*) from
 Hawaii using quantitative PCR. We manipulated the presence and absence of
 song as a cue about sperm competition risk and replicated the study in
 males of two morphs (normal-wing and flatwing), populations (O'ahu
 and Kaua'i). We used a synthetic chorus; individual songs used to
 make the chorus, as well as MATLAB code to assemble a synthetic chorus,
 are provided. ### Files and variables #### File:
 qPCR_Results\_Master\_wide.csv **Description:**  ##### Variables * ID: The
 cricket's ID number. Numbers beginning with O denote crickets from
 O'ahu while numbers beginning with W denote males from Wailua,
 Kaua'i. * Plate: The number of the plate/batch this cricket was
 processed with. * CTMean_Actin: Mean cycle threshold value for actin, the
 housekeeping gene. * CTMean_Gene1262: Mean cycle threshold value for
 isotig1262. * DeltaCTMean1262: Difference in CTMean between this gene and
 actin. * TwoToMinusDCT1262: Transformed CTMean value according to
 convention (2^-DeltaCTMean) * CTMean_Gene11709: Mean cycle threshold value
 for isotig1709. * DeltaCTMean1709: Difference in CTMean between this gene
 and actin. * TwoToMinusDCT1709: Transformed CTMean value according to
 convention (2^-DeltaCTMean) * CTMean_Gene1832: Mean cycle threshold value
 for isotig1832. * DeltaCTMean1832: Difference in CTMean between this gene
 and actin. * TwoToMinusDCT1832: Transformed CTMean value according to
 convention (2^-DeltaCTMean) * Population: The cricket's population of
 origin, either O'ahu or Kaua'i. * AcousticTreatment: The
 presence or absence of synthetic cricket chorus (cue about sperm
 competition risk) in the environment during development. Possible values
 are Song (present) and NoSong (absent). * Morph: Male wing morph. Possible
 values are normal-wing or flatwing. * AgeDAE: The cricket's age in
 days after eclosion. We sacrificed all males on Day 14 after eclosion, so
 all of these values are 14. * Pronotum width (mm) 1: Measurement of the
 pronotum width in millimeters by observer 1. * Pronotum width (mm) 2:
 Measurement of the pronotum width in millimeters by observer 2. *
 BodyMass_mg: The cricket's body mass in milligrams. * TestesMass_mg:
 The cricket's testes mass in milligrams. #### File:
 SFP\_Data\_Analysis\_CommentedForDryad.R **Description:** The R script
 used to produce the analyses used in the manuscript. Comments are provided
 to explain each section. Includes figure generation code. #### File:
 Individual_Songs.zip **Description:** A compressed file containing all the
 individual songs made to mimic songs with O'ahu and Kaua'i
 population-specific properties. These songs were used to generate the
 synthetic cricket chorus. #### File:
 Synthetic\_Toceanicus_Chorus_-\_Kauai\_and\_Oahu\_mix\_-\_12\_hrs\_-\_corrected.wav **Description:** A .wav file containing 12 hours of synthetic chorus (1 unique hour looped to create a 12 hour stimulus). #### File: Toceanicus\_SFP\_Acoustic\_Treatment\_Generation.m **Description:** The MATLAB code used to create the 12-hour acoustic stimulus we used to manipulate perceived sperm competition risk. ## Code/software MATLAB version 2023b R version 4.0.4 (2021-02-15) "Lost Library Book". All required packages are listed and loaded in the header.