DOI: 10.5281/zenodo.6401552
title: Elevated temperature and carbon dioxide levels alter growth rates and shell composition in the fluted giant clam, Tridacna squamosa

description: We investigated the effects of 60-d exposure to end-of-the-century projections for seawater temperature (+3 °C) and <em>p</em>CO<sub>2</sub> (+500 µatm) on growth, mineralogy, and organic content of shells and scutes in juvenile <em>Tridacna squamosa </em>clams. The provided excel file contains tables supplying the following raw datasets: (1) Concentrations of trace minerals (Data_Minerals) in shells and scutes <strong>IndividualID</strong> - unique ID for each juvenile <em>Tridacna squamosa</em> clam<br> <strong>Type</strong> - type of skeletal material sampled (New: newly-formed, Old: older-growth, Shell: shell, Scute: scute).<br> <strong>Exposure</strong> - experimental treatments (Ambient: ambient seawater conditions, High Temp: elevated temperature alone, High pCO2: elevated <em>p</em>CO<sub>2</sub> alone, Multistressor: elevated temperature and <em>p</em>CO<sub>2</sub> in combination).<br> <strong>SampleMass_mg</strong> - mass (in mg) of powdered skeletal material analyzed.<br> <strong>X_ppb</strong> - concentration (in parts per billion) of element X (Mg: Magnesium, Si: Silica, P: Phosphorus, K: Potassium, Mn: Manganese, As: Arsenic, Sr: Strontium, Ba: Barium, Ca: Calcium) in the sample.<br> <strong>X_mmol_kg-1</strong> - concentration (in mmol kg<sup>-1</sup>) of element X in the sample.<br> <strong>XCaRatio_mmol_mol-1</strong> - ratio of the concentrations of element X (in mmol) and calcium (in mol).<br> <strong>BaCaRatio_µmol_mol-1 </strong>- ratio of the concentrations of barium (in µmol) and calcium (in mol). (2) Morphometric characteristics (Data_Morphometry) of shells/scutes <strong>IndividualID</strong> - unique ID for each juvenile <em>Tridacna squamosa</em> clam<br> <strong>Exposure</strong> - experimental treatments (Ambient: ambient seawater conditions, High Temp: elevated temperature alone, High pCO2: elevated <em>p</em>CO<sub>2</sub> alone, Multistressor: elevated temperature and <em>p</em>CO<sub>2</sub> in combination).<br> <strong>X_pre_mm</strong> - measurement value (in millimeters) prior to exposure to experimental treatments (APM: anterior-posterior margin or shell length, DVM: dorsal-ventral margin or shell height, OrnamentationMaxWidth: maximum width of the shell across the scutes, Width: shell width).<br> <strong>X_pre_g</strong> - measurement value (in grams) prior to exposure to experimental treatments (WetMass: whole animal wet mass).<br> <strong>X_post_mm</strong> - measurement value (in millimeters) after 60-d exposure to experimental treatments.<br> <strong>X_post_g</strong> - measurement value (in grams) after 60-d exposure to experimental treatments.<br> <strong>X_gain_mm</strong> - change in measurment value (in millimeters) over the course of the 60-d experiment.<br> <strong>X_gain_%change</strong> - change in measurment value over the course of the 60-d experiment as a percentage of the initial, pre-exposure value (i.e., % change). (3) Organic content (Data_Organics) of shells <strong>IndividualID</strong> - unique ID for each juvenile <em>Tridacna squamosa</em> clam<br> <strong>Exposure</strong> - experimental treatments (Ambient: ambient seawater conditions, High Temp: elevated temperature alone, High pCO2: elevated <em>p</em>CO<sub>2</sub> alone, Multistressor: elevated temperature and <em>p</em>CO<sub>2</sub> in combination).<br> <strong>X-newgrowth_weight%</strong> - concentration of element X (Nitrogen, Carbon, or Hydrogen) as a percentage of the weight of sample analyzed in newly-formed shell.<br> <strong>X-oldgrowth_weight% </strong>- concentration of element X as a percentage of the weight of sample analyzed in older-growth shell.