cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/xnk2t-afa84
title: JV6781_ΔdotLOB

description: ΔdotLOB (Deletion of Dot/Icm ATPases dotL, dotO, dotB)
description: <strong>Tilt Series Date:</strong> 2017-07-17</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~2.6</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain at OD~ 2.6, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-17-31/rawdata/</p>20170717_JV67810035.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-17-31/rawdata/20170717_JV67810035.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170717_JV67810035_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-17-31/3dimage_86941/20170717_JV67810035_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9gk0e-hzd23
title: Agro_AD1484

description: Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid).Cb resistance. Grown on induction plate pH5.8
description: <strong>Tilt Series Date:</strong> 2015-10-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid). Cells were scraped off the induction plate (particularly from the immediate surface of the plate: this is to ensure that if something is happening on the real surface of the plate, we do not miss it), dissolved in induction media, spun for 15 min in a table top centrifuge, supernatant thrown away, cells redissolved in PBS. Add gold to this: 1ul of gold to 4 ul of cells and freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-74/3dimage_70947/</p>20151026_AD1484_30005_full.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-74/3dimage_70947/20151026_AD1484_30005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20151026_AD1484_30005.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-74/rawdata/20151026_AD1484_30005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hxm8j-20934
title: Agro_AD1484

description: Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid).Cb resistance. Grown on induction plate pH5.8
description: <strong>Tilt Series Date:</strong> 2015-10-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid). Cells were scraped off the induction plate (particularly from the immediate surface of the plate: this is to ensure that if something is happening on the real surface of the plate, we do not miss it), dissolved in induction media, spun for 15 min in a table top centrifuge, supernatant thrown away, cells redissolved in PBS. Add gold to this: 1ul of gold to 4 ul of cells and freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-9/3dimage_70805/</p>20151026_AD1484_20001_full.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-9/3dimage_70805/20151026_AD1484_20001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20151026_AD1484_20001.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-10-26-9/rawdata/20151026_AD1484_20001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.30007459
title: Source Data

description: This is the dataset used in the paper titled "scDILT: A Model-Based and Constrained Deep Learning Framework for Single-Cell Data Integration, Label Transferring, and Clustering", including the following SLN208.h5, SLN111.h5, PBMC.h5, HumanLung.h5 and Granulocyte.h5.
DOI: 10.6084/m9.figshare.30007459.v1
title: Source Data

description: This is the dataset used in the paper titled "scDILT: A Model-Based and Constrained Deep Learning Framework for Single-Cell Data Integration, Label Transferring, and Clustering", including the following SLN208.h5, SLN111.h5, PBMC.h5, HumanLung.h5 and Granulocyte.h5.
DOI: 10.22002/9scdt-jp245
title: JV7091

description: JV7091 is a double mutant that lacks dotE and dotP (Lp02).Cell density was quite low on the grid!
description: <strong>Tilt Series Date:</strong> 2016-01-12</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV7091 at OD= 2.9 Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-12-43/3dimage_72548/</p>7091_area_atlas11_100010001.rec,  Reconstruction (Pixel Size 1.68 nm), 861.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-12-43/3dimage_72548/7091_area_atlas11_100010001.rec"
        > <i class="download icon"></i> Download </a></p>

        7091_area_atlas11_100010001.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-12-43/rawdata/7091_area_atlas11_100010001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0s75z-23d53
title: Agro_AD1408_from plate_20150831

description: Agro_AD1408_GFP-VirB11 (Kan+AS plates, Mes pH5.8). Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD1408) and wash the plate by gently rolling the liquid. I had 20ug/ml Kan to keep the cells happier. Add gold to this: 1ul of gold to 4 ul of cells and freeze.
description: <strong>Tilt Series Date:</strong> 2015-08-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow AD1408 cells on AB solid medium (pH 7) at 30C. For induction, use one single colony and spread on induction plates (AB Mes pH 5.8) containing Kan (20ug/ml)+AS (200µM). Grow at 19C for 48 hr. Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD1408) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-31-12/3dimage_61037/</p>AD1408_plate0009_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-31-12/3dimage_61037/AD1408_plate0009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        AD1408_plate0009.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-31-12/rawdata/AD1408_plate0009.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0z7bm-ja091
title: JV1644 Legionella thyA ΔdotO

description: JV1644 Legionella thyA ΔdotO. Lacks dotO ATPase.
description: <strong>Tilt Series Date:</strong> 2016-06-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0.</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the JV1644 strain at OD~2.9. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-06-14-98/rawdata/</p>JV1644_150620164th0021.mrc,  Tilt Series (Pixel Size 3.9 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-06-14-98/rawdata/JV1644_150620164th0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV1644_150620164th0021.rec,  Reconstruction (Pixel Size 15.6 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-06-14-98/3dimage_75768/JV1644_150620164th0021.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16976592
title: Sindhi WordNet Dataset

description:  











Sindhi WordNet Dataset

Developed By

Abdul Majid Bhurgri Institute of Language Engineering (AMBILE), HyderabadUnder the administrative control of the Culture, Tourism, Antiquities & Archives Department, Government of Sindh.

Overview

The Sindhi WordNet Dataset contains a collection of Sindhi words, annotated with various linguistic features such as categories, tenses, synonyms, antonyms, and more. This dataset is designed for natural language processing (NLP) tasks, particularly for tasks such as word sense disambiguation, semantic analysis, and syntactic tagging for the Sindhi language.

Dataset Structure

The dataset is provided in CSV format and contains the following columns:



word_id: A unique identifier for each word entry.

word: The Sindhi word.

category: The part of speech or syntactic category of the word (e.g., noun, verb, adjective).

gender: The gender associated with the word (if applicable).

invariants: Information about whether the word is invariant (e.g., whether it has plural or singular forms).

tags: The syntactic or semantic tag associated with the word (e.g., conjunction, preposition).

tenses: The tense information for the word (if applicable).

hyp: Any hypernyms associated with the word.

antonyms: Antonyms for the word (if available).

synonyms: Synonyms for the word (if available).


Example




word_id
word
category
gender
invariants
tags
tenses
hyp
antonyms
synonyms




1
۽
-
-
-
con
-
-
-
-


2
۾
-
-
-
pp
-
-
-
-


3
اَبَدُ
-
-
singular
noun,adv
-
-
-
-


4
اَبَدِي
-
-
singular
adj
-
-
-
-


5
اَبَدِيت
-
-
singular
noun
-
-
-
-




Features



Comprehensive word annotations for various linguistic categories.

Multiple syntactic and semantic features such as tense, gender, synonyms, and antonyms.

Designed for Sindhi NLP tasks, helping improve language processing for the Sindhi language.


Usage

This dataset can be used for a wide range of NLP tasks such as:



Part-of-speech tagging.

Word sense disambiguation.

Semantic analysis.


You can load and process this dataset using any standard CSV reader in your preferred programming language (e.g., Python's pandas).

Acknowledgments

Special thanks to the AMBILE team for their efforts in data curation, cleaning, formatting, and tagging.

Data Source

The dataset is sourced from the AMBILE WordNet project.

License

This dataset is released under the Creative Commons Attribution-NonCommercial 4.0 License. It is intended for educational and research purposes only.

Contact

For any queries, collaboration opportunities, or contributions, please contact:

Email: datasets@sindh.ai
DOI: 10.5281/zenodo.16976593
title: Sindhi WordNet Dataset

description:  











Sindhi WordNet Dataset

Developed By

Abdul Majid Bhurgri Institute of Language Engineering (AMBILE), HyderabadUnder the administrative control of the Culture, Tourism, Antiquities & Archives Department, Government of Sindh.

Overview

The Sindhi WordNet Dataset contains a collection of Sindhi words, annotated with various linguistic features such as categories, tenses, synonyms, antonyms, and more. This dataset is designed for natural language processing (NLP) tasks, particularly for tasks such as word sense disambiguation, semantic analysis, and syntactic tagging for the Sindhi language.

Dataset Structure

The dataset is provided in CSV format and contains the following columns:



word_id: A unique identifier for each word entry.

word: The Sindhi word.

category: The part of speech or syntactic category of the word (e.g., noun, verb, adjective).

gender: The gender associated with the word (if applicable).

invariants: Information about whether the word is invariant (e.g., whether it has plural or singular forms).

tags: The syntactic or semantic tag associated with the word (e.g., conjunction, preposition).

tenses: The tense information for the word (if applicable).

hyp: Any hypernyms associated with the word.

antonyms: Antonyms for the word (if available).

synonyms: Synonyms for the word (if available).


Example




word_id
word
category
gender
invariants
tags
tenses
hyp
antonyms
synonyms




1
۽
-
-
-
con
-
-
-
-


2
۾
-
-
-
pp
-
-
-
-


3
اَبَدُ
-
-
singular
noun,adv
-
-
-
-


4
اَبَدِي
-
-
singular
adj
-
-
-
-


5
اَبَدِيت
-
-
singular
noun
-
-
-
-




Features



Comprehensive word annotations for various linguistic categories.

Multiple syntactic and semantic features such as tense, gender, synonyms, and antonyms.

Designed for Sindhi NLP tasks, helping improve language processing for the Sindhi language.


Usage

This dataset can be used for a wide range of NLP tasks such as:



Part-of-speech tagging.

Word sense disambiguation.

Semantic analysis.


You can load and process this dataset using any standard CSV reader in your preferred programming language (e.g., Python's pandas).

Acknowledgments

Special thanks to the AMBILE team for their efforts in data curation, cleaning, formatting, and tagging.

Data Source

The dataset is sourced from the AMBILE WordNet project.

License

This dataset is released under the Creative Commons Attribution-NonCommercial 4.0 License. It is intended for educational and research purposes only.

Contact

For any queries, collaboration opportunities, or contributions, please contact:

Email: datasets@sindh.ai
DOI: 10.22002/syd0k-13062
title: Agro_AD1526_GFP-VirB11_20150826

description: Agro_AD1526_GFP-VirB11 (Cb+Kan+AS plates, Mes pH5.8). Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1526) and wash the plate by gently rolling the liquid. Take this liquid and add to the other plate. Repeat the same. This should get enough cells dissolved in the liquid.Add gold to this: 1ul of gold to 4 ul of cells.
description: <strong>Tilt Series Date:</strong> 2015-08-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow AD1526 cells on AB solid medium (pH 7) at 30C. For induction, use one single colony and spread on induction plates (AB Mes pH 5.8) containing  Cb (50ug/ml)+Kan (50ug/ml)+AS (200µM). Grow at 19C for 48 hr. Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1526) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-57/3dimage_60871/</p>AD1526tomo0032_part61_1.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-57/3dimage_60871/AD1526tomo0032_part61_1.rec"
        > <i class="download icon"></i> Download </a></p>

        AD1526tomo0032.mrc,  Tilt Series (Pixel Size 0.42 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-57/rawdata/AD1526tomo0032.mrc"
        > <i class="download icon"></i> Download </a></p>