DOI: 10.5061/dryad.pzgmsbcxn
title: Data from: TNFAIP9 protects against the development of the early stage of chronic kidney disease: Focus on inflammation and fibrosis

description: Tumor necrosis factor alpha-induced protein 9 (TNFAIP9) is a crucial
 effector molecule that protects cells from inflammatory and metabolic
 damage. This study focuses on investigating the role and regulatory
 mechanisms of TNFAIP9 in the progression of chronic kidney disease (CKD).
 By analyzing CKD-related datasets from the GEO database, we discovered
 that TNFAIP9 was upregulated in CKD patients and CKD mice compared to
 their normal controls. To elucidate the functional role of TNFAIP9, we
 established a mouse model of CKD through a two-step 5/6 nephrectomy (Nx).
 The experimental mice were transduced with an adenoviral vector to express
 TNFAIP9. The results showed that mice undergoing 5/6-Nx developed evident
 renal impairment, inflammation, and fibrosis. Overexpression of TNFAIP9
 resulted in the remission of renal impairment, a decreased inflammatory
 response, and a reduced expression of fibrotic markers. In vitro, human
 renal tubular epithelial human kidney-2 (HK-2) cells were exposed to tumor
 necrosis factor-alpha (TNF-α) or transforming growth factor-beta (TGF-β)
 to simulate inflammatory and fibrotic conditions, respectively. Then, the
 overexpression plasmid or small interfering RNA (siRNA) targeting TNFAIP9
 was transfected into HK-2 cells to either overexpress or knock down the
 target protein. Overexpression of TNFAIP9 reduced the TNF-α-induced
 inflammatory response, while its knockdown amplified it. Likewise,
 overexpression of TNFAIP9 decreased the TGF-β-induced fibrosis, whereas
 its knockdown heightened it. In summary, it is suggested that TNFAIP9
 plays a protective role against the early stage of CKD by suppressing
 renal inflammation and fibrosis. Therefore, targeting TNFAIP9 could be a
 promising therapeutic approach for CKD.
description: The current dataset provides raw data that can be used to form
 the histogram, including quantitative results of
 immunofluorescence/immunohistochemistry/pathology scores, kit results, and
 normalized quantitative results of PCR and Western blot.
description: # Data from: TNFAIP9 protects against the development of the early stage
 of chronic kidney disease: focus on inflammation and fibrosis
 **Introduction** This dataset includes raw data that can be used to form
 the histogram, including quantitative results of
 immunofluorescence/immunohistochemistry/pathology scores, kit results, and
 normalized quantitative results of PCR and Western blot to support the
 study “TNFAIP9 protects against the development of the early stage of
 chronic kidney disease: focus on inflammation and fibrosis”, which is
 currently in press. **File list** Original Data, in pptx format. **Author
 Information** Name: Ying Chen Institution: First Hospital of China Medical
 University, Shenyang, China Co-investigator 1 Name: Yanqiu Li Institution:
 First Hospital of China Medical University, Shenyang, China Corresponding
 author Name: Dr. Deyu Zhang Institution: Shengjing Hospital of China
 Medical University, Shenyang, China **Description of the data and file
 structure** The data presented in this dataset underpins the quantitative
 statistics for each graph featured in the publication, thereby ensuring
 that the dataset is derived from the figures reported in the publication.
 The included data encompasses the following quantitative results: Kit
 tests yielded serum levels of creatinine and urea nitrogen; Masson’s
 trichrome staining quantified the percentage of fibrotic areas relative to
 the total area. ELISA assays determined concentrations of TNF-α, IL-6, and
 MCP-1 within kidney tissue or cell supernatants. Western blot analysis
 provided relative quantification values based on grayscale measurements
 for α-SMA, Fibronectin, TNFAIP9, iNOS, COX-2, p-p65ser536, and Collagen
 III. The percentage of positively stained areas for TNFAIP9, Collagen III,
 and NGAL was assessed through immunohistochemistry. Real-time PCR
 facilitated measurement of relative mRNA content for TNFAIP9. Biochemical
 assays recorded arterial pressure as well as 24-hour urinary protein
 excretion from the animals involved in this study. Hematoxylin-eosin (HE)
 staining offered a quantitative score reflecting tissue injury severity.
 Additionally, immunofluorescence techniques were employed to quantify the
 percentage of positively stained cells. **Acknowledgments** This study was
 funded by the Postdoctoral Science Foundation of China (2021M703608). The
 funders had no role in study design, data collection and analysis,
 decision to publish, or preparation of the manuscript.