cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/7asj3-kee04
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-8_slicer4336.jpg: Parallel flagella aba2008-07-17-8_slicer4343.jpg: DNA supercoil
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-8/rawdata/</p>Borrelia_15.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-8/rawdata/Borrelia_15.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_15.rec,  Reconstruction (Pixel Size 1.328 nm), 171.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-8/3dimage_2627/Borrelia_15.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e7nbh-1dv95
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-40_slicer4739.jpg: S layer
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-40/rawdata/</p>Borrelia_78.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-40/rawdata/Borrelia_78.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_78.rec,  Reconstruction (Pixel Size 1.328 nm), 226.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-40/3dimage_3540/Borrelia_78.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.aaupv5
title: Occurrence Download

description: A dataset containing 9052 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spodoptera litura"
 ]
}

 The dataset includes 9052 records from 109 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044332-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/f6f5e-njw03
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-46_slicer4774.jpg: S layer .  Peptidoglycan aba2008-07-17-46_slicer4775.jpg: Flagellar motors
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-46/rawdata/</p>Borrelia_86.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-46/rawdata/Borrelia_86.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_86.rec,  Reconstruction (Pixel Size 1.328 nm), 143.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-46/3dimage_3552/Borrelia_86.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.uqvtgh
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pomatorhinus horsfieldii",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044334-250811113504898/datasets/export for details.
DOI: 10.6084/m9.figshare.29983774
title: Regulation of major histocompatibility complex class-I (MHC-I) gene methylation using DNA methyltransferase inhibitor and PD-L1 inhibitor in triple negative breast cancer

description: The relationship between the expression of MHC-I and DNA methylation was analyzed using data from The Cancer Genome Atlas (TCGA). The expression of DNMTs and MHC-I was analyzed using qRT-PCR, and DNA methylation status of MHC-I genes was analyzed using methylation specific PCR in TNBC cell lines. TNBC cell lines were treated with DNMT inhibitors and programmed cell death-ligand 1 (PD-L1) inhibitor. Then, changes in the expression of DNMT and MHC-I, and methylation patterns of MHC-I genes were analyzed.
DOI: 10.6084/m9.figshare.29983774.v1
title: Regulation of major histocompatibility complex class-I (MHC-I) gene methylation using DNA methyltransferase inhibitor and PD-L1 inhibitor in triple negative breast cancer

description: The relationship between the expression of MHC-I and DNA methylation was analyzed using data from The Cancer Genome Atlas (TCGA). The expression of DNMTs and MHC-I was analyzed using qRT-PCR, and DNA methylation status of MHC-I genes was analyzed using methylation specific PCR in TNBC cell lines. TNBC cell lines were treated with DNMT inhibitors and programmed cell death-ligand 1 (PD-L1) inhibitor. Then, changes in the expression of DNMT and MHC-I, and methylation patterns of MHC-I genes were analyzed.
DOI: 10.22002/f8b6b-x6d84
title: Caulobacter CC1319

description: Caulobacter CC1319
description: <strong>Tilt Series Date:</strong> 2007-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christine Jacobs Wagner</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2007-07-17-7/rawdata/</p>CC1319_071707_8.st,  Tilt Series (Pixel Size 0.961 nm), 990.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2007-07-17-7/rawdata/CC1319_071707_8.st"
        > <i class="download icon"></i> Download </a></p>

        CC1319_071707_8_full.rec,  Reconstruction (Pixel Size 1.922 nm), 597.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2007-07-17-7/3dimage_869/CC1319_071707_8_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t70wn-sht80
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-10_slicer4351.jpg: Parallel flagella
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-10/rawdata/</p>Borrelia_17.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-10/rawdata/Borrelia_17.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_17.rec,  Reconstruction (Pixel Size 1.328 nm), 262.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-10/3dimage_2631/Borrelia_17.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g0nf7-cn489
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-35_slicer4691.jpg: Unknown patch aba2008-07-17-35_slicer4693.jpg: DNA supercoil
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-35/rawdata/</p>Borrelia_71.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-35/rawdata/Borrelia_71.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_71.rec,  Reconstruction (Pixel Size 1.328 nm), 218.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-35/3dimage_3530/Borrelia_71.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ccsm8-4kr88
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-39_slicer4727.jpg: peptidoglycan
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-39/rawdata/</p>Borrelia_77.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-39/rawdata/Borrelia_77.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_77.rec,  Reconstruction (Pixel Size 1.328 nm), 231.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-39/3dimage_3538/Borrelia_77.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bjzz8-7jx58
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-42_slicer4743.jpg: Unknown patch
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-42/rawdata/</p>Borrelia_81.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-42/rawdata/Borrelia_81.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_81.rec,  Reconstruction (Pixel Size 1.328 nm), 147.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-42/3dimage_3544/Borrelia_81.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/k9pqp-1yt45
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-16_slicer4402.jpg: Parallel flagella aba2008-07-17-16_slicer4404.jpg: Flagellar motor .  DNA supercoil
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-16/rawdata/</p>Borrelia_44.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-16/rawdata/Borrelia_44.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_44.rec,  Reconstruction (Pixel Size 1.328 nm), 204.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-16/3dimage_2641/Borrelia_44.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b8sb9-5v003
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-41_slicer4740.jpg: s layer aba2008-07-17-41_slicer4742.jpg: Extracellular vesicles
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-41/rawdata/</p>Borrelia_80.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-41/rawdata/Borrelia_80.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_80.rec,  Reconstruction (Pixel Size 1.328 nm), 234.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-41/3dimage_3542/Borrelia_80.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5v3a6-trg15
title: Borrelia burgdorferi

description: Borrelia burgdorferi
description: <strong>Tilt Series Date:</strong> 2008-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Borrelia burgdorferi</p>
<strong>Strain:</strong> B31</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
  aba2008-07-17-37_slicer4711.jpg: extracellular vesicles aba2008-07-17-37_slicer4712.jpg: Peptidoglycan
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-37/rawdata/</p>Borrelia_74.st,  Tilt Series (Pixel Size 0.664 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-37/rawdata/Borrelia_74.st"
        > <i class="download icon"></i> Download </a></p>

        Borrelia_74.rec,  Reconstruction (Pixel Size 1.328 nm), 188.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2008-07-17-37/3dimage_3534/Borrelia_74.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g6fp4-v4w47
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-27</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-27-60/rawdata/</p>atlas_10001.mrc,  Tilt Series (Pixel Size 0.39 nm), 363.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-27-60/rawdata/atlas_10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_10001.rec,  Reconstruction (Pixel Size 1.56 nm), 222.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-27-60/3dimage_88903/atlas_10001.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_10009_full.rec,  Reconstruction (Pixel Size 1.615 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-27-60/3dimage_90275/atlas_10009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_10001_full.rec,  Reconstruction (Pixel Size 1.615 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-27-60/3dimage_90276/atlas_10001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/934pc-3ep30
title: V. cholerae CF59 - ∆tcpB pMT5

description: Classical strain with ctxA deletion.Minor pilin tcpB knockout.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-06-17</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-17-31/3dimage_73291/</p>20150617_AK08_10031_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
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        20150617_AK08_10031.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
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        20150617_AK08_10031_SIRT_2K_cropped.rec,  magenta arrow: strange hollow spheres .   .  green arrow: filamentous structure (from some viewing angles resembles a twisted ladder or a washboard).  This structure may contain DNA. .   .  Both structures are also visible in: ab2015-06-02-1 ab2015-06-02-29 .  [2015-11-21 00:18:08] -andrew, 2.1 GB  
        
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        ycw2015-06-17-31_slicer14024.png,  556.5 kB  
        
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