DOI: 10.22002/58eqf-fxn09
title: Caulobacter crescentus

description: wild type CB15N. 2 of 8, no warping. mid-divisional cell constriction site + stalk end; In Curv filament + FtsZ filaments; very dense 'medusa-head' feature; amorphous density near stalk
description: <strong>Tilt Series Date:</strong> 2006-06-22</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; 5 days growth; see DPD notebook Jun8-12, 2006</p>
<strong>Sample Preparation:</strong> box 694, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006</p>
  SAVE_blackmass_xy_01.jpg: file associated to 3D Image #304 SAVE_blackmass_yz_01.jpg: file associated to 3D Image #304 SAVE_ftsZ_yz_01.jpg: file associated to 3D Image #304 SAVE_InCurv_xy_01.jpg: file associated to 3D Image #304 SAVE_InCurv_xy_02.jpg: file associated to 3D Image #304 SAVE_InCurv_xz_01.jpg: file associated to 3D Image #304 SAVE_InCurv_xz_02.jpg: file associated to 3D Image #304 pdd2006-06-22-2_slicer10039.jpg: Caulobacter crescentus .  peptidoglycan .  s-layer pdd2006-06-22-2_slicer10040.jpg: Caulobacter crescentus .  extracellular vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-2/rawdata/</p>06Jun22t02.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-2/rawdata/06Jun22t02.mrc"
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        06Jun22t02bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 671.1 MB  
        
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        trim.rec,  Reconstruction (Pixel Size 2.4 nm), 10.5 MB  
        
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        06Jun22t02_13kx.dm3,  * associated to rawdata, 8.6 MB  
        
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        SAVE_blackmass_xy_01.tif,  file associated to 3D Image #304, 6.0 MB  
        
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        SAVE_blackmass_yz_01.tif,  file associated to 3D Image #304, 6.0 MB  
        
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        SAVE_ftsZ_yz_01.tif,  file associated to 3D Image #304, 6.1 MB  
        
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        SAVE_InCurv_xy_01.tif,  file associated to 3D Image #304, 6.1 MB  
        
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        SAVE_InCurv_xy_02.tif,  file associated to 3D Image #304, 6.0 MB  
        
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        SAVE_InCurv_xz_01.tif,  file associated to 3D Image #304, 6.0 MB  
        
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        SAVE_InCurv_xz_02.tif,  file associated to 3D Image #304, 5.9 MB  
        
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        06Jun22t02.mov,  file associated to 3D Image #304, 537.0 MB  
        
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        Jun22t02sm.mov,  file associated to 3D Image #304, 20.4 MB  
        
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