DOI: 10.5061/dryad.tc5g4
title: Data from: Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells

description: Although a great deal is known about the signaling events that promote
 nuclear translocation of NF‐κB, how cellular biophysics and the
 microenvironment might regulate the dynamics of this pathway is poorly
 understood. In this study, we used high‐content image analysis and
 Bayesian network modeling to ask whether cell shape and context features
 influence NF‐κB activation using the inherent variability present in
 unperturbed populations of breast tumor and non‐tumor cell lines.
 Cell–cell contact, cell and nuclear area, and protrusiveness all
 contributed to variability in NF‐κB localization in the absence and
 presence of TNFα. Higher levels of nuclear NF‐κB were associated with
 mesenchymal‐like versus epithelial‐like morphologies, and RhoA‐ROCK‐myosin
 II signaling was critical for mediating shape‐based differences in NF‐κB
 localization and oscillations. Thus, mechanical factors such as cell shape
 and the microenvironment can influence NF‐κB signaling and may in part
 explain how different phenotypic outcomes can arise from the same chemical
 cues.
description: SUM159Files are 16-bit tiffs
 (which means they will appear black if opened in Preview, Photoshop, or
 similar program). Each file is a Multi-plane tiff, containing three
 fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB
 (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 =
 DHE (dihydroethidium, hydroethidine; Sigma) File names refer to
 [row][column]-[field] See ReadMe file for culture and staining
 information.ZR75.1Files are 16-bit tiffs
 (which means they will appear black if opened in Preview, Photoshop, or
 similar program). Each file is a Multi-plane tiff, containing three
 fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB
 (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 =
 DHE (dihydroethidium, hydroethidine; Sigma) File names refer to
 [row][column]-[field] See ReadMe file for culture and staining
 information.T47DFiles are 16-bit tiffs
 (which means they will appear black if opened in Preview, Photoshop, or
 similar program). Each file is a Multi-plane tiff, containing three
 fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB
 (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 =
 DHE (dihydroethidium, hydroethidine; Sigma) File names refer to
 [row][column]-[field] See ReadMe file for culture and staining
 information.SUM149Files are 16-bit tiffs
 (which means they will appear black if opened in Preview, Photoshop, or
 similar program). Each file is a Multi-plane tiff, containing three
 fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB
 (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 =
 DHE (dihydroethidium, hydroethidine; Sigma) File names refer to
 [row][column]-[field] See ReadMe file for culture and staining
 information.SKBR3Files are 16-bit tiffs
 (which means they will appear black if opened in Preview, Photoshop, or
 similar program). Each file is a Multi-plane tiff, containing three
 fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB
 (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 =
 DHE (dihydroethidium, hydroethidine; Sigma) File names refer to
 [row][column]-[field] See ReadMe file for culture and staining
 information.MDA-MB-453Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.MDA-MB-231Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]MCF12A-no EGFFiles are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]MDA-MB-157Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]MCF10A-no EGFFiles are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]MCF7Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]JIMT1Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]hs578TFiles are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]HCC1954Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]HCC1143Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]HCC70Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]CAMA1Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]BT474Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]AU565Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]Hep3BFiles are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]HeLaFiles are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]AG1573 fibroblastFiles are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]