DOI: 10.5061/dryad.dbrv15f3j
title: Nitrogen availability and plant functional composition modify biodiversity-multifunctionality relationships

description: The ability of an ecosystem to deliver multiple functions at high levels
 (multifunctionality) typically increases with biodiversity but there is
 substantial variation in the strength and direction of biodiversity
 effects, suggesting context-dependency. A better understanding of the
 drivers of this context dependency is essential to predict effects of
 global change on ecosystems. To determine how different factors modulate
 the effect of diversity on multifunctionality, we established a large
 grassland experiment with 216 communities, crossing a manipulation of
 plant species richness (1, 4, 8, 20 species) with manipulations of
 resources (nitrogen enrichment), plant functional composition (gradient in
 mean specific leaf area [SLA] to manipulate abundances of exploitative,
 fast-growing vs. conservative, slow-growing species), plant functional
 diversity (variance in SLA) and enemy abundance (foliar fungal pathogen
 removal). We measured ten above- and belowground functions, related to
 productivity, nutrient cycling and energy transfer between trophic levels,
 and calculated ecosystem multifunctionality. Plant species richness and
 functional diversity both increased multifunctionality, but their effects
 were context dependent. Species richness increased multifunctionality only
 when communities were assembled with fast growing (high SLA) species. This
 was because slow species were more redundant in their functional effects,
 whereas different fast species tended to promote different functions.
 Functional diversity also increased multifunctionality but this effect was
 dampened by nitrogen enrichment. However, unfertilised, functionally
 diverse communities still delivered more functions than low diversity,
 fertilised communities. Our study suggests that a shift towards
 fast-growing exploitative communities will not only alter ecosystem
 functioning but also the strength of biodiversity-functioning
 relationships, which highlights the potentially complex effects of global
 change on multifunctionality.
description: Datasets used in "Nitrogen availability and plant functional
 composition modify biodiversity-multifunctionality
 relationships"  from Noémie A. Pichon, Seraina L.
 Cappelli, Tosca Mannall, Thu Zar Nwe, Norbert Hölzel, Valentin H. Klaus,
 Till Kleinebecker, Santiago Soliveres, Hugo Vincent and Eric
 Allan Initial manuscript can be found on bioRxiv:
 https://www.biorxiv.org/content/10.1101/2020.08.17.254086v1 
 This experiment manipulates in a full factorial design species
 richness (1, 4, 8 species), nitrogen enrichment (0, 100 kg ha-1 year-1),
 fugicide spraying (unsprayed, sprayed) and initial community composition
 in SLA (gradient in growth strategies). See methods for
 more info on the design. If you have further questions:
 noemie.pichon@oulu.fi
description: All_BigData.txt contains information on the ecosystem functions
 measured sp_presence contains information on the
 species present in each plot Mean_SLA.txt contains
 information on the specific leaf area of each species
description: # Nitrogen availability and plant functional composition modify
 biodiversity-multifunctionality relationships
 MULTIFUNCTIONALITY
 DATASETS Datasets used in "Nitrogen availability and plant functional
 composition modify biodiversity-multifunctionality relationships"
 from Noémie A. Pichon, Seraina L. Cappelli, Tosca Mannall, Thu Zar Nwe,
 Norbert Hölzel, Valentin H. Klaus, Till Kleinebecker, Santiago Soliveres,
 Hugo Vincent and Eric Allan Initial manuscript can be found on bioRxiv:
 https://www.biorxiv.org/content/10.1101/2020.08.17.254086v1 This
 experiment manipulates in a full factorial design species richness (1, 4,
 8 species), nitrogen enrichment (0, 100 kg ha-1 year-1), fugicide spraying
 (unsprayed, sprayed) and initial community composition in SLA (gradient in
 growth strategies). See methods for more info on the design. If you have
 further questions: noemie.pichon@wsl.ch These datasets can be used with
 code on GitHub: https://github.com/noemiepichon/multifunctionality To
 calculate multifunctionality, please use the code in GitHub
 "Megamodel github.R" To calculate species effect on functioning,
 please use the code in "Sp effect on function github.R"
 Attention: the functions were transformed before calculating
 multifunctionality. See methods. In the first dataset
 "All_BigData.txt", we put together the ten functions measured on
 each plot of the experiment, and each plot community metrics (SLA,
 richness etc). All_BigData.txt: Plot_Nr Block 1 to 4 Species_richness 1 to
 20 Functional_composition F, M, S, for Fast, mixed or slow initial sown
 composition Nitrogen 0 or 1 Fungicide 0 or 1 Combination unique
 combination of species, growing once in control conditions, once with
 nitrogen, once with fungicide, once with both CWM_SLA Community specific
 leaf area calculated with monoculture SLA values and plot-specific
 abundances MPD_SLA_abundance Mean pairwise distance in specific leaf area
 calculated with monoculture SLA values and plot-specific abundances
 MPD_SLA_presence Mean pairwise distance in specific leaf area calculated
 with monoculture SLA values equally weighted Aboveground_biomass (will be
 sqrt transformed) Herbivory (will be sqrt transformed) Pathogens (no
 transformation needed) Soil_respiration (will be log transformed)
 Plant_N_uptake (will be log transformed) Plant_P_uptake (will be log
 transformed) Belowground_biomass (will be log transformed) BGlucosidase
 (will be sqrt transformed) Phosphatase (will be log transformed)
 Carbon_storage (will be sqrt+1 transformed) The second dataset
 "Mean_SLA.txt" is the mean SLA per control monoculture, averaged
 over the years. Plot_Nr CWM_SLA Community specific leaf area calculated
 with monoculture SLA values and plot-specific abundances Species
 Abbreviation for species identity The last dataset
 "species_presence.txt" indicates if a species is present (1) or
 absent (0) of a plot. Am Achillea millefolium Ao Anthoxanthum odoratum As
 Anthriscus sylvestris Be Bromus erectus Cb Crepis biennis Cj Centaurea
 jacea Dc Daucus carotta Dg Dactylis glomerata Fr Festuca rubra Ga Galium
 album Hl Holcus lanatus Hp Helictotrichon pubescens Hs Heracleum
 sphondylium Lp Lolium perenne Pg Prunella grandiflora Pm Plantago media Pt
 Poa trivialis Ra Rumex acetosa Sp Salvia pratensis To Taraxacum officinale
 Block 1 to 4 Plot_Nr