DOI: 10.5281/zenodo.16737205
title: Data sets for Dissecting the Phenotypic Complexity of Biofilm Development and Quorum Sensing in Salmonella enterica Serovars- Figure 2,3,4,5 and 6 growth curves, CV assay and OD measurements  raw data.
title: Growth Curve OD570 Measurements of Salmonella enterica Strains Treated With Quorum Sensing and Quenching Molecules Figure 3
title: Crystal Violet Biofilm Assay OD570 Measurements of Salmonella enterica and E. coli Treated With AHLs and Farnesol at Varying Concentrations Figure 4
title: Growth Curve OD570 Measurements of Salmonella and Control Strains With and Without C8HSL Treatment- Figure 2
title: SE Biofilm Growth on Abiotic Surface ± QSM (Figure 5)
title: Biofilm Formation on Caco-2 Cells ± Quorum Sensing and Quenching Molecules (Figure 6)

description: Figure 2- This dataset contains OD570 absorbance values measured over a 14-hour period to assess bacterial growth kinetics in triplicate for seven strains: Salmonella enterica serovars (SE, ST14028, STA1357, STWT, SV), E. coli, and P. aeruginosa. Data includes two experimental conditions: untreated control and treatment with the quorum sensing molecule N-octanoyl homoserine lactone (C8HSL). Measurements were taken hourly and include biological triplicates. The dataset includes raw values and clearly labeled treatment groups. 

Figure 3- This dataset presents OD570 absorbance measurements of Salmonella enterica serovar Typhimurium 14028 and S. enteritidis strains grown under treatment with various quorum sensing (QS) and quorum quenching (QQ) molecules. Each strain was exposed to C6HSL, C8HSL, C12HSL (AHL-type QS signals), and farnesol (a known quorum quencher), alongside untreated controls. Measurements were taken every hour for 10 hours, with three biological replicates per condition. OD570 values reflect bacterial growth kinetics and provide insight into how different signaling molecules influence growth and QS-mediated behavior.

Figure 4- This dataset presents OD570 absorbance values from crystal violet biofilm quantification assays conducted on Salmonella enterica strains (SE, ST14028, SV, STWT) and E. coli. Each strain was treated with one of five signaling molecules: C4HSL, C6HSL, C8HSL, C12HSL (acyl-homoserine lactones), or the quorum quenching compound farnesol. Treatments were applied at three concentrations (0.05 mM, 0.2 mM, and 1 mM), and each condition includes three biological replicates. The data supports analysis of biofilm modulation in response to quorum sensing and quorum quenching agents across multiple bacterial strains.

Fgiure 5- This dataset contains OD570 measurements from a crystal violet biofilm assay evaluating Salmonella Enteritidis (SE) biofilm formation on an abiotic surface. SE was treated with quorum sensing molecules (QSM), specifically C4-homoserine lactone (C4HSL) and C6-homoserine lactone (C6HSL), at two concentrations (0.2 mM and 0.05 mM). Each condition includes three biological replicates. The dataset allows comparative analysis of biofilm biomass formation in response to QSM treatment versus untreated controls. Statistical significance indicators (ns, *, **) are included based on visual interpretation of the original figure.

Figure 6- This dataset contains OD590 absorbance values from a crystal violet biofilm assay assessing biofilm formation on Caco-2 epithelial cells. The experiment evaluates the effect of quorum sensing molecules (C4HSL, C6HSL) and the quorum quenching compound farnesol at three concentrations: 95%, 0.2 mM, and 0.05 mM. Controls without treatment are included for comparison. Each condition includes three biological replicates. The dataset supports analysis of quorum sensing influence on host cell-associated biofilm development and the potential biofilm-disruptive effect of farnesol.