DOI: 10.5061/dryad.h44j0zpsj
title: Polystyrene-induced changes in adipose transcriptome

description: Microplastics (MP) derived from the weathering of polymers, or synthesized
 in this size range, have become widespread environmental contaminants and
 have found their way into water supplies and the food chain. Despite this
 awareness, little is known about the health consequences of MP ingestion.
 We have previously shown that the consumption of polystyrene (PS) beads
 was associated with intestinal dysbiosis, diabetes, and obesity in mice.
 To further evaluate the systemic metabolic effects of PS on the
 gut-liver-adipose tissue axis, we supplied C57BL/6J mice with normal water
 or that containing two sizes of PS beads (0.5mm and 5mm) at a
 concentration of 1mg/ml. After 13wk, we evaluated several indices of
 metabolism and liver function. As previously observed, mice drinking the
 PS-containing water had a potentiated weight gain and adipose expansion.
 Here we found that this was associated with an increased abundance of
 adipose F4/80+ macrophages. These exposures did not cause non-alcoholic
 fatty liver disease (NAFLD) but were associated with decreased liver:body
 weight ratios and an enrichment in hepatic FXR and LXR signaling. PS also
 increased hepatic cholesterol and altered both hepatic and cecal bile
 acids. Mice consuming PS beads and treated with the berry anthrocyanin,
 delphinidin, demonstrated an attenuated weight gain compared to those mice
 receiving a control intervention and also exhibited a down-regulation of
 cAMP and PPAR signaling pathways. This study highlights the obesogenic
 role of PS in perturbing the gut-liver-adipose axis and altering nuclear
 receptor signaling and intermediary metabolism. Dietary interventions may
 limit the adverse metabolic effects of PS consumption.  
description: To perform the adipose transcriptomic analysis, RNA was prepared
 from tissue sections using a miRNeasy kit (Qiagen). Concentrations were
 determined using a NanoDrop instrument (Thermo Scientific). RNAseq and
 data analysis utilizing the Kyoto Encyclopedia of Genes and Genomes (KEGG,
 Gene Ontology classifications (GO), and the Reactome Database was
 performed by Novogene.
description: # adipose RNA seq data 
 RNAseq data from epidydimal white adipose tissue isolated from 2 mouse
 treatment groups: group1-mice consuming water containing 0.5 micron
 polystyrene beads and injected with DMSO group2- mice consuming water
 containing 0.5 micron polystyrene beads and injected with delphinidin ##
 Description of the data and file structure Two Excel files listing the
 differentially expressed genes (DEGs) are supplied. One file lists those
 genes which are up-regulated in the delphinidin group versus the DMSO
 group (TO-DELPvsDMSO_up.xlsx). The second file lists those genes which are
 down-regulated in the delphinidin group versus the DMSO group
 (TO-DELPvsDMSO_down.xlsx). In each Excel file, there are two tabs listing
 the DEGs. One tab lists in order of fold change, while the other lists in
 order of p-value. As columns in each tab and in each file, there are: A:
 level in the delphinidin group; B: level in the DMSO group; C:log2 fold
 change; D: p value; E: adjusted p value; F: gene name; G: gene biotype; H:
 gene description. ## Sharing/Access information n/a ## Code/Software n/a