DOI: 10.6078/d15t4m
title: Disease scores and microbial densities for seedlings in phage pre-treatment trials

description: This dataset contains two files of results associated with a project
 testing phage pre-treatment of tomato seedlings prior to infection with
 Pseudomonas syringae pv. tomato strain DC3000. One file contains
 the disease scores of each seedling over time, and the other contains
 bacteria and phage densities at the endpoint of sampling. Bacterial
 densities were quantified using two methods (colony forming units and
 droplet digital PCR), and phage densities were quantified solely with
 droplet digital PCR.
description: <strong>Disease scores file</strong>: This file
 contains the disease scores for inoculated seedlings in all three trials.
 Inoc_date is the inoculation date, Sample is the seedling/tube number
 within a given trial, Treatment is the inoculation treatment, Day is day
 post-inoculation, and Disease_score is the disease score on the specified
 day for each sample (following the scoring scheme of Morella et al. 2019).
 Treatment abbreviations are as follows: DC3000 only is “DC3000”,  DC3000
 with phage FRS is “DC_FRS”, DC3000 with phage SHL is “DC_SHL”, DC3000 with
 autoclaved phage FRS is “DC_Autoclaved_FRS”, phage FRS only is “FRS”,
 phage SHL only is “SHL”, autoclaved phage FRS only is
 “AutoclavedFRS_ONLY”, and the magnesium chloride control is
 “MG_NEG”. <strong>Densities
 file: </strong>This file contains the bacterial densities (in colony
 forming units and droplet digital PCR copies) and phage densities (in
 droplet digital PCR copies) adjusted for seedling weights in all trials.
 Inoc_date is the inoculation date, Sample is the seedling/tube number
 within a given trial, and Treatment is the inoculation treatment.
 Treatment abbreviations are as follows: DC3000 only is “DC3000”,  DC3000
 with phage FRS is “DC_FRS”, DC3000 with phage SHL is “DC_SHL”, DC3000 with
 autoclaved phage FRS is “DC_Autoclaved_FRS”, phage FRS only is “FRS”,
 phage SHL only is “SHL”, autoclaved phage FRS only is
 “AutoclavedFRS_ONLY”, and the magnesium chloride control is “MG_NEG”.
 Colony forming unit (CFU) values are expressed per milliliter of seedling
 homogenate (“CFU_nonadjusted_permlhomog”), and per gram and milligram of
 seedling (“CFU_pergram” and “CFU_permg” respectively). ddPCR columns
 specify if they represent the value for bacteria (any column beginning
 with “ddPCR_B”) or phage P1 (any column beginning with “ddPCR_P”). ddPCR
 columns with “raw” at the end are copies per µL reaction from the droplet
 reader. ddPCR values with “adj_pergram” at the end have had the following
 calculation performed: raw values from reader x 10 (for sample dilution in
 water) x 10 (for dilution of sample in ddPCR reaction) x 1000 (to
 calculate copies per mL) * volume (in mL) seedling was homogenized in /
 weight of seedling in grams. “adj_permg” columns are the “adj_pergram”
 values divided by 1000. “rawpermg” columns are the copies per uL reaction
 from the droplet reader (“raw” columns), multiplied by the volume (in mL)
 the seedlings were homogenized in, divided by the seedling weight in
 milligrams.   In the June 25 trial
 (trial 1), two samples were removed due to caps falling off during
 seedling scoring.  Sample 13 was removed on the first day, and has no
 disease scores or densities associated with it. Sample 3 has disease
 scores for the first 3 days, but no further scores or densities.