DOI: 10.5061/dryad.s4mw6m9hp
title: Data from: Legume life history interacts with land use degradation of rhizobia: Implications for restoration success

description: Restoration of soil microbial communities, and microbial mutualists in
 particular, is increasingly recognized as critical for the successful
 restoration of grassland plant communities. Although the positive effects
 of restoring arbuscular mycorrhizal fungi during restoration of these
 systems have been well-documented, less is known about the potential
 importance of nitrogen-fixing rhizobium bacteria, which associate with
 legume plant species that comprise an essential part of grassland plant
 communities, to restoration outcomes. In a series of greenhouse and field
 experiments, we examined the effects of disturbance on rhizobium
 communities, how plant interactions with these mutualists changed with
 disturbance, and whether rhizobia can be used to enhance the establishment
 of desirable native legume species in degraded grasslands. We found that
 agricultural disturbance alters rhizobium communities in ways that affect
 the growth and survival of legume species. Native legume species derived
 more benefit from interacting with rhizobia than did non-native species,
 regardless of rhizobia disturbance history. Additionally, slow-growing,
 long-lived legume species received more benefits from associating with
 rhizobia from undisturbed native grasslands than from associating with
 rhizobia from more disturbed sites. Together, this suggests that native
 rhizobia may be key to enhancing the restoration success of legumes in
 disturbed habitats.
description: # Data from: Legume life history interacts with land use degradation of
 rhizobia: implications for restoration success
 [https://doi.org/10.5061/dryad.s4mw6m9hp](https://doi.org/10.5061/dryad.s4mw6m9hp) ## Description of the data and file structure \# Data sets used in: "Legume life history interacts with land use degradation of rhizobia: implications for restoration success" Author list: Susan M. Magnoli James D. Bever Description of the experimental efforts: Data were collected from two greenhouse experiments, one field experiment, and one observational study. ### Files and variables #### File: Magnoli\_Bever\_Greenhouse1.csv **Description:** This dataset contains all data points used in our analysis of greenhouse experiment 1. The variables are described as follows: ##### Variables * plant: individual plant * experiment: which experiment (a or b) an individual plant was in * site: site where microbial inoculant originated * type: land use type * species: plant species * lifehist: plant life history * alive: whether a plant was alive at the time of harvest * nodules: number of root nodules a plant produced; n/a values for dead plants * nodweight: mass (g) of 10 nodules (if plant produced less than 10 nodules, mass of that many nodules); n/a values for dead plants * estweight: estimated mass (g) of nodules based on nodules and nodweight; n/a values for dead plants * aboveground: aboveground plant biomass (g); n/a values for dead plants * belowground: belowground plant biomass (g); n/a values for dead plants * totalbiomass: aboveground + belowground (g); n/a values for dead plants #### File: Magnoli\_Bever2024\_Greenhouse2.csv **Description:** This dataset contains all data points used in our analysis of greenhouse experiment 2. The variables are described as follows: ##### Variables * plant: individual plant * Species: plant species * LifeHistory: plant life history * Rhizobia: land use type from where rhizobia originated * Replicate: plant replicate within Species/Rhizobia combination * alive: whether a plant was alive at the time of harvest * nodules: number of root nodules a plant produced; n/a values for dead plants * contamination: whether a control plant (uninoculated) produced nodules; n/a values for dead plants * nodweight: mass (g) of 10 nodules (if plant produced less than 10 nodules, mass of that many nodules); n/a values for dead plants or uninoculated control plants * estnodweight: estimated mass (g) of nodules based on nodules and nodweight; n/a values for dead plants or uninoculated control plants * shoot: aboveground plant biomass (g); n/a values for dead plants * root: belowground plant biomass (g); n/a values for dead plants * totalbiomass: totalbiomass: aboveground + belowground (g); n/a values for dead plants #### File: Magnoli\_Bever2024\_RhizobiaRelAb.csv **Description:** This dataset contains all data points used in our analysis of nitrogen fixer relative abundance. The variables are described as follows: ##### Variables * site: site * land_use: land use type * abundance: total abundance of N-fixer OTUs in a given site/land sample * fixer_relab: relative abundance of N-fixer OTUs in a given site/land use sample #### File: Magnoli\_Bever2024\_RhizobiaOTU.csv **Description:** This dataset is an OTU matrix. The first column contains sample IDs. All following columns contain counts of individual OTUs. ##### Variables * sample: * 0b9c0aec02c50d6707695c8fdeb9367b: * c847f0718bc04a2c7dd9af2b123c9974: * 6b2b5f5a8e66afcaa442555f41d28cdc: * 98c686f7caf06f4aa2d9387da5f02b70: * 42eabf92fd1ef412a8f1471d675b5f18: * f9daa1567c9798eb1a14635b005a0817: * 2d42ce473d2b03a0120c12db61e4dcae: * c4a8ea85a855e282d69ba97a00453b20: * 12ae77f47a0f38edbbf6a84a2972d155: * 267d23917503a126cb810f09348ba09c: * 98fc74d168c51a7ac9784b9f6e5b7dcb: * 953c1e47352b55c89dcaf1689f341b2e: * 2d5b915aab686f696c61a176e2d4c5ef: * af955694e0efeb1a2357bcde3b9b46b6: * de0e648a38655ca3a56ac00cbf542dc2: * df225bc21b49ade0cd132b882d8f32f5: * b06f187da400600c43f2450e54ee3e21: * cb46a0e634a74884144f4c9a714a23a7: * a5d161e279d3182510e7aed2c76206b0: #### File: Magnoli\_Bever2024\_OTU\_metadata.csv **Description:** This dataset contains metadata for the Magnoli_Bever2024_RhizobiaOTU.csv file. The variables are described as follows: ##### Variables * sample: sample ID * sample_id: sample ID * site: site from which sample was taken * landuse: land us type at sample site #### File: Magnoli\_Bever2024\_field.csv **Description:** This dataset contains all data points used in the analysis of our field experiment. The variables are described as follows: ##### Variables * block: experimental block * plot: plot within block * plant: individual plant number within plot * amf: AM fungal treatment * rhiz: Rhizobia treatment * inoc: whether a plant was inoculated with rhizobia * rhizsource: site and land use origin of rhizobia inoculants * site: site origin of rhizobia inoculants * species: plant species * alive: whether a plant was alive or dead * leafnumber: number of leaves on plant; n/a values for dead plants or species for which this trait was not measured * stemdiam: stem diameter (mm) of plant; n/a values for dead plants or species for which this trait was not measured * biomass: aboveground biomass (g) of plant; n/a values for dead plants or species for which this trait was not measured * rootmass: belowground biomass (g) of plant; n/a values for dead plants or species for which this trait was not measured * alive_year2: whether plant was alive in year 2 of experiment; n/a values for species that were harvested in year 1 * alive_year3: whether plant was alive in year 3 of experiment; n/a values for species that were harvested in year 1