cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/05vwk-7ay43
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-207/rawdata/</p>JV9082_201808290207.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-207/rawdata/JV9082_201808290207.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_201808290207_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-207/3dimage_96805/JV9082_201808290207_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vfwnd-0zt46
title: Legionella IcmQ71::TevS 42C_On

description: Legionella IcmQ71::TevS 42C_On (HS004 E)
description: <strong>Tilt Series Date:</strong> 2019-05-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Howard Shuman (University of Chicago). Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-53/rawdata/</p>HS004E-area8-tomo0006.mrc,  Tilt Series (Pixel Size 0.26 nm), 996.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-53/rawdata/HS004E-area8-tomo0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        HS004E-area8-tomo0006_part35_2.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-53/3dimage_103748/HS004E-area8-tomo0006_part35_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7q882d
title: Occurrence Download

description: A dataset listing the 15 species recorded in GBIF matching the query:
{
 "DatasetKey" : [
 "is Forestry Inventory 2015"
 ]
}

 The dataset's 15 records were derived from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007978-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.16997885
title: Mapping Nursing Contributions to Early-Stage Breast Cancer Survivorship: A Bibliometric Review
DOI: 10.5281/zenodo.16997886
title: Mapping Nursing Contributions to Early-Stage Breast Cancer Survivorship: A Bibliometric Review
DOI: 10.22002/prbtt-hjk52
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-266/rawdata/</p>JV9082_20180901a0040.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-266/rawdata/JV9082_20180901a0040.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0040_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-266/3dimage_96949/JV9082_20180901a0040_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8zgjnu
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Copadichromis diplostigma, Copadichromis geertsi, Copadichromis ilesi, Copadichromis insularis, Copadichromis jacksoni, Copadichromis likomae, Copadichromis mbenjii, Copadichromis melas, Copadichromis mloto, Copadichromis nkatae, Copadichromis parvus, Copadichromis pleurostigma, Copadichromis pleurostigmoides, Copadichromis quadrimaculatus, Copadichromis trewavasae, Copadichromis trimaculatus, Copadichromis verduyni, Copadichromis virginalis, Coptodon bakossiorum, Coptodon bemini, Coptodon bythobates, Coptodon cameronensis, Coptodon camerunensis, Coptodon coffea, Coptodon congica, Coptodon dageti, Coptodon deckerti, Coptodon discolor, Coptodon ejagham, Coptodon flava, Coptodon fusiforme, Coptodon guineensis, Coptodon gutturosa, Coptodon imbriferna, Coptodon ismailiaensis, Coptodon kottae, Coptodon louka, Coptodon margaritacea, Coptodon nigrans, Coptodon nyongana, Coptodon rendalli, Coptodon rheophila, Coptodon snyderae, Coptodon spongotroktis, Coptodon tholloni, Coptodon thysi, Coptodon walteri, Coptodon zillii, Coptostomabarbus bellcrossi, Coptostomabarbus wittei, Corematodus shiranus, Corematodus taeniatus, Coryogalops william, Cotylopus acutipinnis, Cotylopus rubripinnis, Crenimugil buchanani, Crenimugil crenilabis, Crenimugil seheli, Cromeria nilotica, Cromeria occidentalis, Ctenochromis horei, Ctenochromis luluae, Ctenochromis oligacanthus, Ctenochromis polli, Ctenogobius lepturus, Ctenopharyngodon idella, Ctenopharynx intermedius, Ctenopharynx nitidus, Ctenopharynx pictus, Ctenopoma acutirostre, Ctenopoma ashbysmithi, Ctenopoma kingsleyae, Ctenopoma machadoi, Ctenopoma multispine, Ctenopoma maculatum, Ctenopoma muriei, Ctenopoma nebulosum, Ctenopoma nigropannosum, Ctenopoma ocellatum, Ctenopoma petherici, Ctenopoma weeksii, Cunningtonia longiventralis, Cyathochromis obliquidens, Cyathopharynx furcifer, Cyclopharynx fwae, Cyclopharynx schwetzi, Cynotilapia afra, Cynotilapia aurifrons, Cynotilapia axelrodi, Cynotilapia chilundu, Cynotilapia pulpican, Cynotilapia zebroides, Microchromis zebroides, Cyphomyrus discorhynchus, Cyphomyrus macrops, Hippopotamyrus macrops, Cyphomyrus psittacus, Hippopotamyrus psittacus, Cyphomyrus wilverthi, Hippopotamyrus wilverthi)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((56.2582 -7.6751,56.5989 -7.542,56.789 -7.1822,56.7426 -6.9401,55.8366 -6.9401,55.7807 -7.0342,55.8667 -7.4864,56.2582 -7.6751))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007994-250827131500795/datasets/export for details.
DOI: 10.22002/j6rze-4hp34
title: Ecoli_EPEC_8612

description: These are E coli cells (EPEC 8612) obtained from Ritesh (Ilan Rosenshine lab at the Hebrew  .  university). The idea is to image bacterial nanotubes in these cells.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> These cells were prepared with Ritesh in Ilan Rosenshine lab at the Hebrew University. </p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. Then washed with PBS and blotted and then plunge . frozen. Yuxi helped in sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-39/rawdata/</p>EPEC_8612_tilt31.mrc,  Tilt Series (Pixel Size 0.449 nm), 480.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-39/rawdata/EPEC_8612_tilt31.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt31.mrc.mdoc,  8.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-39/rawdata/EPEC_8612_tilt31.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3wwee-fdy52
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-323/rawdata/</p>JV9082_20180901a0097.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-323/rawdata/JV9082_20180901a0097.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0097_part37_4.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-323/3dimage_97030/JV9082_20180901a0097_part37_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9eezr-9yw65
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-289/rawdata/</p>JV9082_20180901a0063.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-289/rawdata/JV9082_20180901a0063.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0063_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-289/3dimage_96996/JV9082_20180901a0063_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5kjdp-ghc34
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-31/rawdata/</p>PginWT_8_014.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-31/rawdata/PginWT_8_014.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_014_part41_3.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-31/3dimage_114430/PginWT_8_014_part41_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xv3fm-zat37
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-46/rawdata/</p>PginWT_8_078.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-46/rawdata/PginWT_8_078.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_078_part40_1.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-46/3dimage_114456/PginWT_8_078_part40_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jsr27-q6997
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-84/rawdata/</p>PginWT_8_097.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-84/rawdata/PginWT_8_097.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_097_full.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-84/3dimage_114530/PginWT_8_097_full.rec"
        > <i class="download icon"></i> Download </a></p>