DOI: 10.5061/dryad.5m4dn73
title: Data from: Faster speciation of fig-wasps than their host figs leads to decoupled speciation dynamics

description: The genus Ficus (Moraceae) is best known for its obligate mutualism with
 pollinating fig-wasps (Agaonidae), where species are thought to
 reciprocally trigger genetic differentiation resulting in tight
 co-speciation. Here, we used nextRAD DNA sequencing to study the
 population structure of multiple fig species and their corresponding
 fig-wasps along an elevational gradient in Papua New Guinea. Contrary to
 the expected one-to-one species specificity in this mutualism we find
 evidence of multiple pollinating wasp species, which through limited
 dispersal abilities along the gradient, likely limit pollen flow and
 influence fig population structure along these slopes. In two cases, where
 the fig species studied have wide distribution along the mountain, we
 found between three and four wasp species pollinating closely related
 populations of fig species. In the case of one fig subspecies complex, we
 identified two fig-wasp species according to the distribution of their
 host subspecies. Finally, in a parapatric, three sister species complex,
 we identified three individual wasp species, each corresponding to its
 host fig species. Fig-wasps appear to speciate more rapidly through faster
 generation times, faster rates of local adaptation and/or weak dispersal
 abilities compared to figs. This in turn restricts pollen movement between
 fig ecotypes, strengthening reproductive barriers and so facilitating
 their speciation. Fig speciation along the gradient and wasp lineage
 extinction may eventually restore the one-to-one rule in this mutualism
 through split and sort speciation dynamics.
description: F. arfarkensis vcf
 fileVCF file for Ficus
 arfakensis samples used for calculating FST using VCFTools, and
 FineRADstructure (after conversion to TSV file according to custom
 script).arffig.vcfCeratosolen ex F. arfakensisVCF file for Ceratosolen sp. samples from F. arfakensis used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).arf.vcfwasfigVCF file for Ficus wassa samples used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).Kradibia ex F. wassaVCF file for Kradibia spp. samples from Ficus wassa used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).was.vcfF. trichocerasa VCF fileVCF file for Ficus trichocerasa samples used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).trifig.vcfCeratosolen ex F. trichocerasaVCF file for Ceratosolen sp. samples from Ficus trichocerasa used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).tri.vcfF. itoana complex VCF fileVCF file for Ficus itoana complex samples used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).itofig.vcfCeratosolen ex F. itoana complexVCF file for Ceratosolen sp. samples from the F. itoana complex used for calculating FST using VCFTools, and FineRADstructure (after conversion to TSV file according to custom script).ito.vcfKradibia wasps FASTA alignmentKradibia wasps alignment used for RAxML phylogeniessequenceAlignment.fastaCeratosolen wasps FASTA alignmentCeratosolen wasps alignment used for RAxML phylogeniessequenceAlignment.fastaCeratosolen ex F. arfakensis COI alignmentCOI sequences from Ceratosolen wasps from F. arfakensis for jMOTU analysisarf.fasCeratosolen ex F. trichocerasa COI alignmentCOI sequences from Ceratosolen wasps from F. trichocerasa for jMOTU analysistri_2.fasKradibia ex F. wassa COI alignmentCOI sequences from Kradibia wasps from F. wassa for jMOTU analysiswas_2.fas