cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14272/tzkirfqawqsrbv-uhfffaoysa-l/chmo0000156
title: X-ray diffraction (XRD) ()
title: InChI=1S/3C17H14NP.Ag.Cu.2HI/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;;;/h3*1-14H;;;2*1H/q;;;2*+1;;/p-2

description: dataset for X-ray diffraction (XRD)
DOI: 10.14272/tzkirfqawqsrbv-uhfffaoysa-l/chmo0000563
title: fast-atom bombardment mass spectrometry (FABMS) ()
title: InChI=1S/3C17H14NP.Ag.Cu.2HI/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;;;/h3*1-14H;;;2*1H/q;;;2*+1;;/p-2

description: dataset for fast-atom bombardment mass spectrometry (FABMS)
 
 MS (FAB, 3-NBA), m/z (%): 589 (1) [CuL2]+, 516 (1) [Cu2LI]+, 460 (5) [3-NBA], 307 (33) [3-NBA], 262 (1) [L-H]+, 154 (100) [3-NBA], 137 (68) [3-NBA]. Due to the poor solubility of the complex, only some bulk signals could be assigned.
DOI: 10.25502/1k81-d724/d
title: BD West-Bangladesh Household processed survey 2023 CIMMYT

description: Dataset processed of a household level survey to describe the main farm characteritics, production and resources allocation in three regions of country in West (Nawabganj, Rajshashi), collected on December 2022 -June 2023.
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000889.1
title: proton-decoupled 31P nuclear magnetic resonance spectroscopy (31P{1H}-NMR) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for proton-decoupled 31P nuclear magnetic resonance spectroscopy (31P{1H}-NMR)
 
 31P{1H} NMR (162 MHz, DMSO-d6, ppm) δ = -4.38 (bs, 3P). Impurities: 3% of the corresponding phosphine oxide at 18.6 ppm.
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0001075
title: elemental analysis (EA) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for elemental analysis (EA)
 
 Anal. calcd. for C51H42ClCuN3P3 (%): C, 68.92; H, 4.76; N, 4.73. Found: C, 67.73; H, 4.71; N, 4.72.
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000630
title: infrared absorption spectroscopy (IR) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for infrared absorption spectroscopy (IR)
 
 IR (ATR, ṽ) = 3043 (vw), 1571 (w), 1481 (w), 1448 (w), 1433 (m), 1419 (w), 1185 (vw), 1153 (vw), 1093 (w), 1047 (vw), 1027 (vw), 987 (w), 766 (w), 740 (m), 692 (m), 618 (w), 505 (m), 444 (w), 409 (w) cm–1.
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000596.1
title: distortionless enhancement with polarization transfer (DEPT) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for distortionless enhancement with polarization transfer (DEPT)
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000595
title: 13C nuclear magnetic resonance spectroscopy (13C NMR) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for 13C nuclear magnetic resonance spectroscopy (13C NMR)
 
 13C NMR (101 MHz, CDCl3, ppm) δ = 160.0 (d, JCP = 35.1 Hz, 3C, CqL(Pyr)), 150.4 (d, JCP = 12.7 Hz, 3C, CL(Pyr)), 135.8 (d, JCP = 6.2 Hz, 3C, CL(Pyr)), 134.4 (d, JCP = 15.5 Hz, 12C, CL(Ph)), 133.7 (d, JCP = 19.0 Hz, 6C, CqL(Ph)), 129.8 (d, JCP = 23.0 Hz, 3C, CL(Pyr)), 129.5 (s, 6C, CL(Ph)), 128.5 (d, JCP = 8.6 Hz, 12C, CL(Ph)), 123.1 (s, 3C, CL(Pyr)).
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000593.1
title: 1H nuclear magnetic resonance spectroscopy (1H NMR) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for 1H nuclear magnetic resonance spectroscopy (1H NMR)
 
 1H NMR (400 MHz, CDCl3, ppm) δ = 8.52 (bs, 3H, HL(Pyr)), 7.45–7.41 (m, 18H, HL(Pyr), HL(Ph)), 7.31–7.27 (m, 6H, HL(Ph)), 7.21–7.17 (m, 12H, HL(Ph)), 7.12–7.08 (m, 3H, HL(Pyr)). Impurities: 8% DCM at 5.29 ppm, 4% water at 1.65 and 10% pentane in the range 0.75-1.40 ppm.
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000596
title: distortionless enhancement with polarization transfer (DEPT) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for distortionless enhancement with polarization transfer (DEPT)
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000726
title: high resolution fast-atom bombardment mass spectrometry (HRFABMS) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for high resolution fast-atom bombardment mass spectrometry (HRFABMS)
 
 MS (FAB, 3-NBA), m/z (%): 950 (3) [M+Cu]+, 852 (9) [M−Cl]+, 687 (7) [Cu2ClL2]+, 589 (83) [CuL2], 460 (5) [3-NBA], 423 (5) [Cu2ClL]+, 326 (52) [CuL]+, 307 (37) [3-NBA], 264 (40) [L+H]+, 185 (13) [PPh2]+, 154 (100) [3-NBA], 137 (63) [3-NBA].
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000889
title: proton-decoupled 31P nuclear magnetic resonance spectroscopy (31P{1H}-NMR) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for proton-decoupled 31P nuclear magnetic resonance spectroscopy (31P{1H}-NMR)
 
 31P{1H} NMR (162 MHz, CDCl3, ppm) δ = -3.20 (s, 3P).
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000593
title: 1H nuclear magnetic resonance spectroscopy (1H NMR) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for 1H nuclear magnetic resonance spectroscopy (1H NMR)
 
 1H NMR (400 MHz, DMSO-d6 [2.50 ppm], ppm) δ = 8.61 (bs, 3H, HL(Pyr)), 7.69 (t, JHH = 7.4 Hz, 3H, HL(Pyr)), 7.41–7.28 (m, 36H, HL(Pyr), HL(Ph)). Impurities: 8% DCM at 5.73 ppm, 4% water at 3.31 ppm and 10% pentane in the range 0.70-1.35.
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m.1
title: C51H42ClCuN3P3
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: This is a physical chemical entity[CHEBI_24431] associated with a molecule[CHEBI_25367].
 The molecule[CHEBI_25367] can be described by the following structural desciptors[cheminf_000085]:
 InChI descriptor[cheminf_000113]: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1, and canonical SMILES descriptor[cheminf_000007]: c1ccc(cc1)P(c1ccccn1)c1ccccc1.c1ccc(cc1)P(c1ccccn1)c1ccccc1.c1ccc(cc1)P(c1ccccn1)c1ccccc1.Cl[Cu], and by the IUPAC name[cheminf_000107]: .
 
 The physical chemical entity[CHEBI_24431] has a component solvent[CHEBI_46787] which is described by the canonical SMILES descriptor[cheminf_000007]:
 
 The physical chemical entity[CHEBI_24431] has the following Sample ID as registered in the research data repository chemotion (www.chemotion-repository.net, https://doi.org/10.25504/FAIRsharing.iagXcR): CRS-36036

 The physical chemical entity[CHEBI_24431] can be described by the physical descriptors [CHEMINF_000025]:
 Melting point descriptor[CHEMINF_000256]: 172.0 (°C)
 Boiling point descriptor[CHEMINF_000257]: 
 Refractive index descriptor[CHEMINF_000253]:

 The physical chemical entity[CHEBI_24431] can be further described by the following assays[OBI:0000070][CHMO:0001133]:
 
 CHMO:0000889 | proton-decoupled 31P nuclear magnetic resonance spectroscopy (31P{1H}-NMR)
 
 CHMO:0000596 | distortionless enhancement with polarization transfer (DEPT)
 
 CHMO:0000889 | proton-decoupled 31P nuclear magnetic resonance spectroscopy (31P{1H}-NMR)
 
 CHMO:0001075 | elemental analysis (EA)
 
 CHMO:0000595 | 13C nuclear magnetic resonance spectroscopy (13C NMR)
 
 CHMO:0000596 | distortionless enhancement with polarization transfer (DEPT)
 
 CHMO:0000630 | infrared absorption spectroscopy (IR)
 
 CHMO:0000726 | high resolution fast-atom bombardment mass spectrometry (HRFABMS)
 
 CHMO:0000156 | X-ray diffraction (XRD)
 
 CHMO:0000593 | 1H nuclear magnetic resonance spectroscopy (1H NMR)
 
 CHMO:0000593 | 1H nuclear magnetic resonance spectroscopy (1H NMR)
 
 The physical chemical entity[CHEBI_24431] was deposited to the Molecule Archive of the Karlsruhe Insitute of Technology (KIT) with the following Sample ID: 

 Used ontologies:
 CHEBI - Chemical Entities of Biological Interest
 CHEMINF - chemical information ontology (information entities about chemical entities)
 CHMO - Chemical Methods Ontology
 OBI - Ontology for Biomedical Investigations
DOI: 10.14272/pyupnhhjwpnkiz-uhfffaoysa-m/chmo0000156
title: X-ray diffraction (XRD) ()
title: InChI=1S/3C17H14NP.ClH.Cu/c3*1-3-9-15(10-4-1)19(16-11-5-2-6-12-16)17-13-7-8-14-18-17;;/h3*1-14H;1H;/q;;;;+1/p-1

description: dataset for X-ray diffraction (XRD)
DOI: 10.6084/m9.figshare.25053224.v1
title: genome annotation of <i>Beaufortia pingi</i>

description: genome annotation of <i>Beaufortia pingi</i>
DOI: 10.4121/e0d75aad-6cd1-45dd-a5ec-985e399337b4
title: Morality is Non-Binary: Building a Pluralist Moral Sentence Embedding Space using Contrastive Learning - models

description: We train embedding spaces with the MFTC corpus, to see how an embedding space can learn the distribution of pluralist morality. We compare off-the-shelf, unsupervised, and supervised approaches, showing that a supervised approach is necessary. Here, you can find the models we trained with unsupervised and supervised approaches.
DOI: 10.4121/e0d75aad-6cd1-45dd-a5ec-985e399337b4.v1
title: Morality is Non-Binary: Building a Pluralist Moral Sentence Embedding Space using Contrastive Learning - models

description: We train embedding spaces with the MFTC corpus, to see how an embedding space can learn the distribution of pluralist morality. We compare off-the-shelf, unsupervised, and supervised approaches, showing that a supervised approach is necessary. Here, you can find the models we trained with unsupervised and supervised approaches.
DOI: 10.5281/zenodo.10497977
title: Cut&Run H3K9ac inVivo HFD Control

description: For CUT&RUN against H3K27ac in mouse liversamples, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-9521048) according to the manufacturer’s instructions with minor modifications.For in vivo experiments, approximately 200 mg of liver tissue was homogenized in DMEM(Gibco, 41966-029) using a potter tube and a glass pestle with 12 strokes. The resulting cellsuspension was then centrifuged at 1000 xg for 5 minutes at 4°C. The samples weresubsequently washed with 1 ml PBS (Sigma D8537) supplemented with cOmplete proteaseinhibitor cocktail (Roche, CO-RO SKU 11697498001) and 10 mM sodium butyrate (SigmaAldrich, 303410), followed by centrifugation under the same conditions. The following stepswere performed in presence of protease inhibitors and sodium butyrate. Washed cells wereresuspended in 1 ml of NIB with 0.3% IGEPAL CA-630 (Sigma, I8896) and incubated on icefor 5 minutes. The nuclear suspension was then centrifuged at 1000 xg for 5 minutes at 4°C.The resulting nuclear pellet was washed with 1 ml of nucleus isolation buffer (NIB) (15 mMTris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mMCaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (CarlRoth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth,Page 37Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) without IGEPAL CA-630,and the nuclei were subsequently filtered through a 30 μm strainer. An additional washing stepwas performed with 1 ml of NIB, and after centrifugation, and the nuclei resuspended in 100μl of NIB without IGEPAL CA-630. The nuclei were counted, and 0.5 x 106 nuclei were usedfor the subsequent procedure, either in step 7 for ACLY ChIC (Version 1.0 EpiCypher, SKU:14-1048) or in step 14 for H3K9ac and H3K27ac ChIC (Version 3.3 EpiCypher, SKU: 14-1048)of the manufacturer's protocol.For each reaction, 2 μg of antibody was used (Diagenode C15410196). Sequencinglibraries were prepared using NEBNext Ultra II library preparation procedure (New EngladBiolabs, E7645L) with modifications according to NGS Library preparation of CUTANAChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048, Version 1.0), and then assessed for qualityand quantity by BioAnalyzer (Agilent). Generated libraries were sequenced on an IlluminaNovaSeq instrument (150 bp, paired-end)
DOI: 10.5281/zenodo.10497978
title: Cut&Run H3K9ac inVivo HFD Control

description: For CUT&RUN against H3K27ac in mouse liversamples, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-9521048) according to the manufacturer’s instructions with minor modifications.For in vivo experiments, approximately 200 mg of liver tissue was homogenized in DMEM(Gibco, 41966-029) using a potter tube and a glass pestle with 12 strokes. The resulting cellsuspension was then centrifuged at 1000 xg for 5 minutes at 4°C. The samples weresubsequently washed with 1 ml PBS (Sigma D8537) supplemented with cOmplete proteaseinhibitor cocktail (Roche, CO-RO SKU 11697498001) and 10 mM sodium butyrate (SigmaAldrich, 303410), followed by centrifugation under the same conditions. The following stepswere performed in presence of protease inhibitors and sodium butyrate. Washed cells wereresuspended in 1 ml of NIB with 0.3% IGEPAL CA-630 (Sigma, I8896) and incubated on icefor 5 minutes. The nuclear suspension was then centrifuged at 1000 xg for 5 minutes at 4°C.The resulting nuclear pellet was washed with 1 ml of nucleus isolation buffer (NIB) (15 mMTris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mMCaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (CarlRoth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth,Page 37Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) without IGEPAL CA-630,and the nuclei were subsequently filtered through a 30 μm strainer. An additional washing stepwas performed with 1 ml of NIB, and after centrifugation, and the nuclei resuspended in 100μl of NIB without IGEPAL CA-630. The nuclei were counted, and 0.5 x 106 nuclei were usedfor the subsequent procedure, either in step 7 for ACLY ChIC (Version 1.0 EpiCypher, SKU:14-1048) or in step 14 for H3K9ac and H3K27ac ChIC (Version 3.3 EpiCypher, SKU: 14-1048)of the manufacturer's protocol.For each reaction, 2 μg of antibody was used (Diagenode C15410196). Sequencinglibraries were prepared using NEBNext Ultra II library preparation procedure (New EngladBiolabs, E7645L) with modifications according to NGS Library preparation of CUTANAChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048, Version 1.0), and then assessed for qualityand quantity by BioAnalyzer (Agilent). Generated libraries were sequenced on an IlluminaNovaSeq instrument (150 bp, paired-end)
DOI: 10.5281/zenodo.10592704
title: A billion years of evolution manifest in nanosecond protein dynamics - DATA

description: Data supporting: "A billion years of evolution manifest in nanosecond protein dynamics"; Proceedings of the National Academy of Sciences (PNAS), 2024.
DOI: 10.5281/zenodo.10592705
title: A billion years of evolution manifest in nanosecond protein dynamics - DATA

description: Data supporting: "A billion years of evolution manifest in nanosecond protein dynamics"; Proceedings of the National Academy of Sciences (PNAS), 2024.
DOI: 10.25502/m81e-sw32/d
title: BD South-Bangladesh Household processed survey 2023 CIMMYT

description: Dataset processed of a household level survey to describe the main farm characteritics, production and resources allocation in three regions of country in South (Barguna, Barisal, Patuakhali), collected on December 2022 -June 2023.
DOI: 10.5281/zenodo.10041137
title: Mountain birch ozone stress bvoc emission
DOI: 10.5281/zenodo.10041138
title: Mountain birch ozone stress bvoc emission
DOI: 10.5281/zenodo.10592071
title: Duferco forecast results using Kernel Ridge

description: The data uploaded represent thirteen months of Duferco forecast (June 2021 - June 2022) using Kernel Ridge regression with hourly granularity for the Calabria wind farm.

The three csv files represents the three different methods developed and tested during the project:



BL: Kernel Ridge regression with gaussian kernel (Baseline).

PC: The Baseline method with the pre-processing of the data using the power curve.

PC+K: The Baseline method with the pre-processing of the data using the power curve, and the post-processing of the forecast using Kalman smoothing filter.
DOI: 10.5281/zenodo.6523894
title: Duferco forecast results using Kernel Ridge

description: The data uploaded represent thirteen months of Duferco forecast (June 2021 - June 2022) using Kernel Ridge regression with hourly granularity for the Calabria wind farm.

The three csv files represents the three different methods developed and tested during the project:



BL: Kernel Ridge regression with gaussian kernel (Baseline).

PC: The Baseline method with the pre-processing of the data using the power curve.

PC+K: The Baseline method with the pre-processing of the data using the power curve, and the post-processing of the forecast using Kalman smoothing filter.
DOI: 10.5281/zenodo.6523895
title: Duferco forecast results using Kernel Ridge

description: Seven months of Duferco forecast (June 2021 - December 2021) using Kernel Ridge regression with hourly granularity for the wind farms of Calabria and Sardinia.
DOI: 10.5281/zenodo.10497985
title: Cut&Run H3K9ac inVivo HFD BA 10mg

description: For CUT&RUN against H3K27ac in mouse liversamples, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-9521048) according to the manufacturer’s instructions with minor modifications.For in vivo experiments, approximately 200 mg of liver tissue was homogenized in DMEM(Gibco, 41966-029) using a potter tube and a glass pestle with 12 strokes. The resulting cellsuspension was then centrifuged at 1000 xg for 5 minutes at 4°C. The samples weresubsequently washed with 1 ml PBS (Sigma D8537) supplemented with cOmplete proteaseinhibitor cocktail (Roche, CO-RO SKU 11697498001) and 10 mM sodium butyrate (SigmaAldrich, 303410), followed by centrifugation under the same conditions. The following stepswere performed in presence of protease inhibitors and sodium butyrate. Washed cells wereresuspended in 1 ml of NIB with 0.3% IGEPAL CA-630 (Sigma, I8896) and incubated on icefor 5 minutes. The nuclear suspension was then centrifuged at 1000 xg for 5 minutes at 4°C.The resulting nuclear pellet was washed with 1 ml of nucleus isolation buffer (NIB) (15 mMTris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mMCaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (CarlRoth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth,Page 37Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) without IGEPAL CA-630,and the nuclei were subsequently filtered through a 30 μm strainer. An additional washing stepwas performed with 1 ml of NIB, and after centrifugation, and the nuclei resuspended in 100μl of NIB without IGEPAL CA-630. The nuclei were counted, and 0.5 x 106 nuclei were usedfor the subsequent procedure, either in step 7 for ACLY ChIC (Version 1.0 EpiCypher, SKU:14-1048) or in step 14 for H3K9ac and H3K27ac ChIC (Version 3.3 EpiCypher, SKU: 14-1048)of the manufacturer's protocol.For each reaction, 2 μg of antibody was used (Diagenode C15410196). Sequencinglibraries were prepared using NEBNext Ultra II library preparation procedure (New EngladBiolabs, E7645L) with modifications according to NGS Library preparation of CUTANAChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048, Version 1.0), and then assessed for qualityand quantity by BioAnalyzer (Agilent). Generated libraries were sequenced on an IlluminaNovaSeq instrument (150 bp, paired-end)
DOI: 10.5281/zenodo.10497986
title: Cut&Run H3K9ac inVivo HFD BA 10mg

description: For CUT&RUN against H3K27ac in mouse liversamples, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-9521048) according to the manufacturer’s instructions with minor modifications.For in vivo experiments, approximately 200 mg of liver tissue was homogenized in DMEM(Gibco, 41966-029) using a potter tube and a glass pestle with 12 strokes. The resulting cellsuspension was then centrifuged at 1000 xg for 5 minutes at 4°C. The samples weresubsequently washed with 1 ml PBS (Sigma D8537) supplemented with cOmplete proteaseinhibitor cocktail (Roche, CO-RO SKU 11697498001) and 10 mM sodium butyrate (SigmaAldrich, 303410), followed by centrifugation under the same conditions. The following stepswere performed in presence of protease inhibitors and sodium butyrate. Washed cells wereresuspended in 1 ml of NIB with 0.3% IGEPAL CA-630 (Sigma, I8896) and incubated on icefor 5 minutes. The nuclear suspension was then centrifuged at 1000 xg for 5 minutes at 4°C.The resulting nuclear pellet was washed with 1 ml of nucleus isolation buffer (NIB) (15 mMTris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mMCaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (CarlRoth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth,Page 37Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) without IGEPAL CA-630,and the nuclei were subsequently filtered through a 30 μm strainer. An additional washing stepwas performed with 1 ml of NIB, and after centrifugation, and the nuclei resuspended in 100μl of NIB without IGEPAL CA-630. The nuclei were counted, and 0.5 x 106 nuclei were usedfor the subsequent procedure, either in step 7 for ACLY ChIC (Version 1.0 EpiCypher, SKU:14-1048) or in step 14 for H3K9ac and H3K27ac ChIC (Version 3.3 EpiCypher, SKU: 14-1048)of the manufacturer's protocol.For each reaction, 2 μg of antibody was used (Diagenode C15410196). Sequencinglibraries were prepared using NEBNext Ultra II library preparation procedure (New EngladBiolabs, E7645L) with modifications according to NGS Library preparation of CUTANAChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048, Version 1.0), and then assessed for qualityand quantity by BioAnalyzer (Agilent). Generated libraries were sequenced on an IlluminaNovaSeq instrument (150 bp, paired-end)
DOI: 10.6084/m9.figshare.25111832
title: SDATA-23-02045-T.zip

description: These are conflict exposure data with country or conflict level plus year delineations from ACLED with WorldPop annual population estimatesUpdated data are available at www.acleddata.com/conflict-exposure
DOI: 10.6084/m9.figshare.25111832.v1
title: SDATA-23-02045-T.zip

description: These are conflict exposure data with country or conflict level plus year delineations from ACLED with WorldPop annual population estimatesUpdated data are available at www.acleddata.com/conflict-exposure