DOI: 10.5061/dryad.8gtht76zh
title: Data from: Increased nitrogen deposition may promote plant invasion through interfering plant-pathogen interactions

description: Intensified anthropogenic activities have led to a documented increase in
 atmospheric nitrogen deposition, which has been shown to impact the growth
 and herbivore resistance of invasive plant species, thereby contributing
 to their invasion success. However, the extent to which elevated nitrogen
 deposition influences plant-pathogen interactions within the context of
 biological invasions remains unexplored. This study initially investigated
 the differences in leaf lesion areas between the invasive weed
 Alternanthera philoxeroides and its local congener, A. sessilis, in
 different terrestrial ecosystems that experienced various levels of
 atmospheric nitrogen deposition. Additionally, a laboratory experiment was
 conducted to assess the changes in pathogen resistance of these two
 species in response to simulated nitrogen deposition, following artificial
 infection with a leaf pathogen. The study also investigated alterations in
 leaf defensive chemicals and phyllosphere microbial communities between
 the two species to elucidate the underlying mechanisms. The findings
 revealed that A. philoxeroides demonstrated superior pathogen resistance
 compared to A. sessilis under both field and laboratory conditions. Leaf
 lesion areas of both species exhibited inverse correlations with
 atmospheric nitrogen deposition levels in the field, as well as with leaf
 nitrogen content in the laboratory. Further correlation analyses and in
 vitro toxicity assessments indicated that the observed differences in
 pathogen resistance between the two species could be attributed to
 opposing changes in leaf total flavonoid concentrations and shifts in the
 composition of the endophytic phyllosphere microbiome in response to
 increased nitrogen deposition. Subsequent microbiome analyses have
 revealed that the phyllosphere of A. philoxeroides accumulated a greater
 abundance of ecologically beneficial microbes within its endophytes, which
 function as biological protectants for the plant. These findings imply
 that elevated atmospheric nitrogen deposition may enhance the invasiveness
 of A. philoxeroides, mediated through interactions between the plant and
 its associated microbes.
description: # Title of Dataset: Increased nitrogen deposition may promote plant
 invasion through interfering plant-pathogen interactions of an invasive
 plant species and its local congener --- Brief summary of dataset
 contents, contextualized in experimental procedures and results. Increased
 nitrogen deposition may promote plant invasion through interfering
 plant-pathogen interactions of an invasive plant species and its local
 congener ## Description of the Data and file structure In this file, there
 are six sheets, which include field survey data, plant growth-related
 traits, leaf chemicals, leaf defensive traits, in vitro toxicity test,
 Shannon index, and competition results from this study. The treatment
 codes were listed as below: * KCK - *A. philoxeroides* under control
 treatment * LCK - *A. sessilis* under control treatment * KN - *A.
 philoxeroides* under simulated nitrogen deposition * LN - *A. sessilis*
 under simulated nitrogen deposition The units of field survey data in
 sheet 1 were percentage of leaf lesion area (%) total amount of atmosphere
 nitrogen deposition (Kg N Ha-1 yr-1) The units of each plant-growth
 related traits in sheet 2 were leaf dry mass(g), Stem dry mass(g), root
 dry mass(g), total dry mass(g), number of leaves, total leaf area(mm2),
 leaf nitrogen content%, leaf carbon content%, leaf water content (%) The
 units of leaf defensive chemicals in sheet 3 were Leaf total flavonoids
 (mg·g-1), leaf tannins content (mg·g-1), leaf total phenolics
 content(mg·g-1) The units of in vitro inhibition test in sheet 4 were
 diameter of *F. oxysporum* (cm) In sheet 5 the Shannon and Chao index did
 not have units The units of competition results in sheet 6 were leaf dry
 mass (g) ## Sharing/access Information Links to other publicly accessible
 locations of the data: The final fungal and bacterial sequence data were
 deposited in the National Center for Biotechnology Information (NCBI)
 database, assigned the SRA accession numbers PRJNA1161130 and
 PRJNA1161039, respectively. Was data derived from another source? No