cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.1r875
title: Data from: Chlorpyrifos interacts with other agricultural stressors to alter stream communities in laboratory microcosms

description: Chlorpyrifos is one of the most widely used agricultural insecticides in
 the world, but to date there is limited empirical information about its
 potential to interact with other common agricultural stressors. We
 conducted a 15-day, community-level microcosm experiment evaluating
 individual and combined effects of chlorpyrifos, nutrient enrichment, and
 sedimentation on stream invertebrate communities (abundance, biomass,
 richness, size structure, composition) and ecosystem processes (primary
 productivity and leaf decomposition). We found that sedimentation was the
 most detrimental stressor, with significant negative impacts on most
 invertebrate community and ecosystem function variables. Even though
 chlorpyrifos did not cause significant invertebrate mortality in the
 microcosms, it still altered ecosystem function by lowering leaf
 decomposition rates, probably through sublethal inhibition of invertebrate
 shredders. Furthermore, we observed a significant reversal interaction
 between chlorpyrifos and sediment for small-sized invertebrates collected
 in gravel (abundance in sediment x insecticide microcosms was 2.4 times
 lower than predicted by additivity), as well as an antagonistic
 interaction with nutrients on invertebrate richness in the same
 microhabitat (richness in nutrient x insecticide microcosms was 1.6 times
 higher than predicted by additivity). Our results suggest that
 chlorpyrifos has the potential to alter freshwater ecosystem function and
 interact non-additively with other common agricultural stressors. These
 findings are in keeping with a growing body of research highlighting that
 multiple stressor interactions and ecosystem processes should be
 considered when evaluating the impacts of organic toxicants on freshwater
 ecosystems.
description: Invertebrate community
 response variablesinvertebrate_metrics.xlsxFunctional response variablesfunction_metrics.xlsxWater quality parameterswater_quality.xlsxNutrient concentrationnutrients.xlsx
DOI: 10.15468/dl.mub2ck
title: Occurrence Download

description: A dataset containing 130 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Nepal",
 "OccurrenceStatus is Present",
 "TaxonKey is Testudines"
 ]
}

 The dataset includes 130 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000965-260126135527185/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5061/dryad.rg25909
title: Data from: Spatial variation in leaf nutrient traits of dominant desert riparian plant species in an arid inland river basin of China

description: Understanding how patterns of leaf nutrient traits respond to groundwater
 depth is crucial for modeling the nutrient cycling of desert riparian
 ecosystems and forecasting the responses of ecosystems to global changes.
 In this study, we measured leaf nutrients along a transect across a
 groundwater depth gradient in the downstream Heihe River to explore the
 response of leaf nutrient traits to groundwater depth and soil properties.
 We found that leaf nutrient traits of dominant species showed different
 responses to groundwater depth gradients. Leaf C, leaf N, leaf P and leaf
 K decreased significantly with groundwater depth, whereas patterns of leaf
 C/N and leaf N/P followed quadratic relationships with groundwater depth.
 Meanwhile, leaf C/P did not vary significantly along the groundwater depth
 gradient. Variations in leaf nutrient traits were associated with soil
 properties (e.g. soil bulk density, soil pH). Groundwater depth and soil
 pH jointly regulated the variation of leaf nutrient traits, however,
 groundwater depth explained the variation of leaf nutrient traits better
 than did soil pH. At the local scale in the typical desert riparian
 ecosystem, the dominant species was characterized by low leaf C, leaf N
 and leaf P, but high leaf N/P and leaf C/P, indicating that, desert
 riparian plants might be more limited by P than N in the growing season.
 Our observations will help to reveal specific adaptation patterns in
 relation to the groundwater depth gradient for dominant desert riparian
 species, provide insights into adaptive trends of leaf nutrient traits,
 and add information relevant to understanding the adaptive strategies of
 desert riparian forest vegetation to moisture gradients.
description: Zhang et al.
 data.xlsMicrosoft Excel was used
 to create this data file, which contains data for Leaf nutrient traits of
 dominant species and soil properties in a desert riparian ecosystem along
 the lower reaches of the Heihe River, China.
DOI: 10.5061/dryad.933v0
title: Data from: Searching for diamonds in the apomictic rough: a case study involving Boechera lignifera (Brassicaceae)

description: The genus Boechera is one of the most difficult species complexes in North
 America, with about 70 sexual diploids and hundreds of apomictic taxa
 representing diverse combinations of nearly every known sexual genome. In
 this study, we set out to clarify the taxonomy of Boechera lignifera,
 which currently includes a small number of sexual diploid populations in
 addition to the widespread apomictic diploid upon which the name is based.
 Using data from cytological studies, microsatellite DNA analyses,
 geography, and morphology, we demonstrate that the apomictic populations
 are genetically quite divergent from the sexual diploids. We propose the
 name Boechera kelseyana to accommodate the sexual diploid taxon, which
 occurs entirely south of the geographic range of B. lignifera. Boechera
 kelseyana is consistently separable from B. lignifera based on pollen and
 seed morphology, the length and proximal orientation of fruiting pedicels,
 differences in the branching and orientation of trichomes on the lowers
 stems, and the number of flowers and cauline leaves on unbranched fertile
 stems.
description: Microsatellite genotypes
 for the 60 samples analyzed in this study.Microsatellite genotypes
 for the 60 samples analyzed in this study. Each microsatellite locus is
 presented in diploid format with the size of each allele in bp. Missing
 data is noted with a -1. Asterisks denote samples that were exact
 multilocus duplicates of other samples at resolved loci. DNA#s correspond
 to Appendix 1.B_kelseyana_lignifera_SSR_data.xlsx
DOI: 10.5061/dryad.836
title: Data from: Sampling strategies for delimiting species: genes, individuals, and populations in the Liolaemus elongatus-kriegi complex (Squamata: Liolaemidae) in Andean-Patagonian South America

description: Recovery of evolutionary history and delimiting species boundaries in
 widely distributed, poorly-known groups requires extensive geographic
 sampling, but this is difficult to design a priori because evolutionary
 diversity is often "hidden" by an inadequate taxonomy. Large
 data sets are needed, and these provide unique challenges for analysis
 when they span intra and inter-specific levels of divergence. Protocols
 have been designed to combine methods of analysis for DNA sequences that
 exhibit both very shallow and relatively deeper divergences (Crandall and
 Fitzpatrick, 1996). In this study we combine several tree-based phylogeny
 reconstruction methods with nested clade analysis, to extract maximum
 historical signal at various levels, in the poorly-known Liolaemus
 elongatus-kriegi complex in temperate South America. We implement the
 basic protocol of Wiens and Penkrot (2002) to test for species boundaries,
 and propose modifications to accommodate large data sets and gene regions
 with heterogeneous substitution rates. Combining haplotype trees with
 nested-clade analyses allowed testing of species boundaries on the basis
 of a priori defined criteria, and this approach suggests that the number
 of putative species could be doubled. We discuss these findings in the
 context of the advantages and limitations of a combined approach for
 retrieval of maximum historical information in large data sets, in the
 context of the yet formidable unresolved issues of sampling strategies.
description: Morando et al Appendix
 1Morando_APPENDIX1.htmMorando et al Appendix 2Morando_APPENDIX2.htm
DOI: 10.5281/zenodo.18239778
title: TIDE I: Evidence for an Effective Geometric Contribution to Gravity in Virialized Galaxy Clusters

description: This record contains the full manuscript and supplementary material for the paper “Rey-TIDE I: Evidence for an Effective Geometric Contribution to Gravity in Virialized Galaxy Clusters.”Included files:• Main manuscript (PDF, v38)• Complete supplementary package (ZIP, v1.0) with datasets, figures, and analysis scripts.

The materials correspond to version 1 of the study (January 2026) and reproduce all results shown in the paper.DOI: 10.5281/zenodo.18239779
DOI: 10.5061/dryad.s0n20
title: Data from: Spatially and temporally varying selection on intrapopulation quantitative trait loci for a life history trade-off in Mimulus guttatus

description: Why do populations remain genetically variable despite strong continuous
 natural selection? Mutation reconstitutes variation eliminated by
 selection and genetic drift, but theoretical and experimental studies each
 suggest that mutation-selection balance insufficient to explain extant
 genetic variation in most complex traits. The alternative hypothesis of
 balancing selection, wherein selection maintains genetic variation, is an
 aggregate of multiple mechanisms (spatial and temporal heterogeneity in
 selection, frequency-dependent selection, antagonistic pleiotropy, etc.).
 Most of these mechanisms have been demonstrated for Mendelian traits, but
 there is little comparable data for loci affecting quantitative
 characters. Here, we report a three-year field study of selection on
 intra-population Quantitative Trait Loci (QTL) of flower size, a highly
 polygenic trait of Mimulus guttatus. The QTL exhibit antagonistic
 pleiotropy: alleles that increase flower size reduce viability but
 increase fecundity. The magnitude and direction of selection fluctuates
 yearly and on a spatial scale of meters. This study provides direct
 evidence of balancing selection mechanisms on QTL of an ecologically
 relevant trait.
description: Phenotype and Fitness
 dataThis file provides the
 raw phenotype and fitness (survivorship and fecundity) data for each of
 the transplanted Nearly Isogenic Lines as described in Mojica et al
 2012.Dryad
 Phenotype and Fitness data_Mojica et al.csv
DOI: 10.5061/dryad.34q04288
title: Data from: Effect of habitat fragmentation on the genetic diversity of peripheral populations of beech in Central Italy

description: Fragmentation can affect the demographic and genetic structure of
 populations near the boundary of their bio-geographic range. Higher
 genetic differentiation among populations coupled with lower level of
 within population variability is expected as a consequence of reduced
 population size and isolation. The effects of these two factors have been
 rarely disentangled. Given their high gene flow, anemophilous forest trees
 should be more affected, in terms of loss of genetic diversity, by small
 population size rather than geographic isolation alone. We studied the
 impact of distance from the main range (a measure of isolation) and
 reduced population size on the within and among population components of
 genetic variability. We assayed 11 isozyme loci in 27 marginal populations
 of European beech (Fagus sylvatica L.) in Central Italy. Populations were
 divided in three groups with an increasing level of fragmentation. In the
 most fragmented group the within population genetic variability was
 slightly smaller and the among population differentiation significantly
 larger than in the other two groups. These results support the role of
 random genetic drift having a larger impact on the most fragmented group,
 while gene flow seems to balance genetic drift in the two less fragmented
 ones. Given that average distance from the main range is not different
 between the intermediate and the most fragmented group, but average
 population size is smaller, we can conclude that gene flow is effective,
 even at relatively long distances, in balancing the effect of
 fragmentation if population size is not too small.
description: Data and
 Readmeperdryad2.txt
DOI: 10.5061/dryad.4gv44
title: Data from: Optimal management strategy of insecticide resistance under various insect life histories: heterogeneous timing of selection and inter-patch dispersal

description: Although theoretical studies have shown that the mixture strategy, which
 uses multiple toxins simultaneously, can effectively delay the evolution
 of insecticide resistance, whether it is the optimal management under
 different insect life histories and insecticide types remains unknown. To
 test the robustness of the management strategy over the life histories, we
 developed a series of simulation models which cover almost all the diploid
 insect types and have the same basic structure describing pest population
 dynamics and resistance evolution with discrete time-steps. For each of
 two insecticidal toxins, one-locus two-allele autosomal inheritance of
 resistance was assumed. The simulations demonstrated the optimality of the
 mixture strategy either when insecticide efficacy was incomplete or when
 some part of the population disperses between patches before mating. The
 rotation strategy, which uses one insecticide on one pest generation and a
 different one on the next, did not differ from sequential usage in the
 time to resistance, except when dominance was low. It was the optimal
 strategy when insecticide efficacy was high and pre-mating selection and
 dispersal occur.
description: ResistanceDLDP_1.1.tarR package source to define functions for the simulation of resistance evolution.how_to_run_simulationProcedures to run the simulation of the resistance evolution and draw plots (Figure 2, 3 and 4 on the article) on your R environment. Installation of "ResistanceDLDP" package is required.run_simulation.R
DOI: 10.5061/dryad.1731
title: Data from: Multiple hybridization events between Drosophila simulans and Drosophila mauritiana are supported by mtDNA introgression

description: The study of speciation has advanced considerably in the last decades due
 to the increased application of molecular tools. In particular the
 quantification of gene flow between recently diverged species could be
 addressed. D. simulans and D. mauritiana diverged, probably
 allopatrically, from a common ancestor approximately 250,000 years ago.
 However, these species share one mitochondrial DNA (mtDNA) haplotype
 indicative of a recent episode of introgression. To study the extent of
 gene flow between these species, we took advantage of a large sample of D.
 mauritiana and employed a range of different markers, i.e. nuclear and
 mitochondrial sequences, and microsatellites. This allowed us to detect
 two new mtDNA haplotypes (MAU3 and MAU4). These haplotypes diverged quite
 recently from haplotypes of the siII group present in cosmopolitan
 populations of D. simulans. The mean divergence time of the most diverged
 haplotype (MAU4) is approximately 127,000 years, which is more than
 100,000 years before the assumed speciation time. Interestingly we also
 found some evidence for gene flow at the nuclear level since an excess of
 putatively neutral loci show significantly reduced differentiation between
 D. simulans and D. mauritiana. Our results suggest that these species are
 exchanging genes more frequently than previously thought.
description: Nunesetal2010MSAmatrixMolEcol.txt
DOI: 10.15468/dl.ufwa86
title: Occurrence Download

description: A dataset containing 44 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pantacantha ameghinoi",
 "HasCoordinate is true",
 "BasisOfRecord is one of (Specimen)"
 ]
}

 The dataset includes 44 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000966-260126135527185/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5061/dryad.9nv1k
title: Data from: Hidden female physiological resistance to male accessory gland substances in a simultaneous hermaphrodite

description: To increase fertilization chances compared to rivals, males are favoured
 to transfer accessory gland proteins to females during mating. These
 substances, by influencing female physiology, cause alteration of her
 sperm usage and remating rate. Simultaneously hermaphroditic land snails
 with love-darts are a case in point. During courtship, a love-dart is
 pierced through the partner's body wall, thereby introducing
 accessory mucous gland products. This mucus physiologically increases
 paternity by inhibiting the digestion of donated sperm. The sperm, which
 are packaged in a spermatophore, are exchanged and received in an organ
 called the diverticulum. Because of its length, this organ was previously
 proposed to be a female anatomical adaptation that may limit the dart
 interference with the recipient's sperm usage. For reproductive
 success of the donor, an anatomically long spermatophore, relative to the
 partner's diverticulum, is beneficial since sperm can avoid digestion
 by exiting through the spermatophore's tail safely. However, the
 snail Eobania vermiculata possesses a diverticulum that is three times
 longer than the spermatophore it receives. Here we report that the
 love-dart mucus of this species contains a contraction-inducing substance
 that shortens the diverticulum, an effect that is only properly revealed
 when the mucus is applied to another helicid species, Cornu aspersum. This
 latter finding suggests that E. vermiculata may have evolved a
 physiological resistance to the manipulative substance received via the
 love-dart by becoming insensitive to it. This provides useful insight into
 evolution of female resistance to male manipulations, indicating that it
 can remain hidden if tested on a single-species.
description: Dose-time
 testMean length of C.
 aspersum’s diverticulum over time when four doses of love-dart mucus of
 the species E. vermiculata and one dose of saline solution were
 applied.Occurence and
 specificity of shortening effectMean length of the
 diverticulum of C. aspersum and E. vermiculata before (0 min.) and after
 (5 min.) five tested substances were applied.Length SPO and
 SROMean length of
 spermatophore- producing and receiving organs of C. aspersum and E.
 vermiculata
DOI: 10.5061/dryad.f352f
title: Data from: Pollution-tolerant invertebrates enhance greenhouse gas flux in urban wetlands

description: One of the goals of urban ecology is to link community structure to
 ecosystem function in urban habitats. Pollution-tolerant wetland
 invertebrates have been shown to enhance greenhouse gas (GHG) flux in
 controlled laboratory experiments, suggesting that they may influence
 urban wetland roles as sources or sinks of GHG. However, it is unclear if
 their effects can be detected in highly variable conditions in a field
 setting. Here we use an extensive dataset on carbon dioxide (CO2), methane
 (CH4), and nitrous oxide (N2O) flux in sediment cores (n = 103) collected
 from ten urban wetlands in Melbourne, Australia during summer and winter
 in order to test for invertebrate enhancement of GHG flux. We detected
 significant multiplicative enhancement effects of temperature, sediment
 carbon content, and invertebrate density on CH4 and CO2 flux. Each
 doubling in density of oligochaete worms or large benthic invertebrates
 (oligochaete worms and midge larvae) corresponded to ~42% and ~15%
 increases in average CH4 and CO2 flux, respectively. However, despite
 exceptionally high densities, invertebrates did not appear to enhance N2O
 flux. This was likely due to fairly high organic carbon content in
 sediments (range: 2.1-12.6%), and relatively low nitrate availability
 (median 1.96 μM NO3--N), which highlights the context-dependent nature of
 community structural effects on ecosystem function. The invertebrates
 enhancing GHG flux in this study are ubiquitous, and frequently dominate
 faunal communities in impaired aquatic ecosystems. Therefore, invertebrate
 effects on CO2 and CH4 flux may be common in wetlands impacted by
 urbanization, and urban wetlands may make greater contributions to the
 total GHG budgets of cities if the negative impacts of urbanization on
 wetlands are left unchecked.
description: isotope data
 4-25-17δ15N and δ13C data for
 sediments and invertebrates collected from urban stormwater treatment
 wetlands and golf course ponds during the winter sampling season.
 Invertebrates were stored overnight in glass vials with water from the
 same sampling site in order to allow for clearance of gut contents, before
 being dried at 60 degrees C for isotope analysis.sediment core data
 4-25-17This file contains
 incubation temperatures, fluxes of dissolved oxygen and greenhouse gases,
 sediment porosity, C and N content, and invertebrate (Oligochaeta and
 Chironomidae) densities for each sediment core collected from urban
 stormwater treatment wetlands and golf course ponds. Corresponding
 environmental data (nutrients, chlorophyll, dissolved oxygen, pH) are
 included as well.Oligochaete subfamily
 densities - selected samples 4-25-17For one
 randomly-selected core per site, Oligochaeta are identified to subfamily
 (occasionally lower taxonomic level), and densities are provided. No worms
 were present in sediment cores collected from the Huntingdale Golf Club
 during the summer sampling season, hence the missing row of
 data.
DOI: 10.5061/dryad.8883h
title: Data from: Environmental variables explain genetic structure in a beetle-associated nematode

description: The distribution of a species is a complex expression of its ecological
 and evolutionary history and integrating population genetic,
 environmental, and ecological data can provide new insights into the
 effects of the environment on the population structure of species.
 Previous work demonstrated strong patterns of genetic differentiation in
 natural populations of the hermaphroditic nematode Pristionchus pacificus
 in its La Réunion Island habitat, but gave no clear understanding of the
 role of the environment in structuring this variation. Here, we present
 what is to our knowledge the first study to statistically evaluate the
 role of the environment in shaping the structure and distribution of
 nematode populations. We test the hypothesis that genetic structure in P.
 pacificus is influenced by environmental variables, by combining
 population genetic analyses of microsatellite data from 18 populations and
 370 strains, with multivariate statistics on environmental data, and
 species distribution modelling. We assess and quantify the relative
 importance of environmental factors (geographic distance, altitude,
 temperature, precipitation, and beetle host) on genetic variation among
 populations. Despite the fact that geographic populations of P. pacificus
 comprise vast genetic diversity sourced from multiple ancestral lineages,
 we find strong evidence for local associations between environment and
 genetic variation. Further, we show that significantly more genetic
 variation in P. pacificus populations is explained by environmental
 variation than by geographic distances. This supports a strong role for
 environmental heterogeneity vs. genetic drift in the divergence of
 populations, which we suggest may be influenced by adaptive forces.
description: dryad_LGThis data file contains the microsatellite (STR) genotypes for 176 Pristionchus pacificus strains collected from La Reunion Island. These strains were genotyped at 19 loci (which included 14 di-nucleotide, four tri-nucleotide, one tetra-nucleotide and one penta-nucleotide repeat), using the primer pairs, protocol and cycling conditions detailed in (Morgan et al., 2012).