DOI: 10.5281/zenodo.13378540
title: Macroscopic, histological and stereological image dataset of Four-Spot megrim (Lepidorhombus boscii) ovaries from the ICES Celtic Seas and Bay of Biscay Ecoregions

description: Contents: 

This dataset contains the macroscopic and histological images of the ovaries of 68 Four-spot megrim (female, Lepidorhombus boscii (Risso, 1810)) collected from the ICES Celtic Seas or Bay of Biscay Ecoregions (Eco) in November 2019 (n=25; Eco=7j), November 2020 (n=16, Eco=7h & 7j), October 2021 (n=12, Eco=8a,b,c) and November 2021 (n=15, Eco=7j) during the annual scientific campaign EVHOE (Evaluation Halieutique Ouest de l'Europe).

 

Images:



Macroscopic_pictures.zip: archive in zip format of 139 pictures (.JPG; 2Mo-6Mo; JPG; 350pp) from 51 female Four-spot megrim dissected during this study. Each photo was taken with a digital camera (no flash). For each individual, up to three pictures were taken when possible (Le Meleder et al., 2022) with : 



one picture of the entire fish with its abdominal cavity open with the ovaries in view

one picture of the whole fish with the ovaries outside of the abdominal cavity

one picture of the ovaries

the name of the picture is the same as the fish's ID number.


Histology_slides.zip : archive in zip format containing the ovarian histological slides digitized using an Aperio CS (Scan Scope Console software, v.10.2.0.2352), x20 lens. The whole slide images (.svs) are of the 218 histological slides acquired during this study. 


 

Data:



QuPath.zip : archive in zip format containing the reading grids for the stereology readings of the histological cross sections of the ovaries under QuPath. For more information on QuPath stereology readings, see Dubroca et al. (2023) in References.



Stereo_BOS_read_me.txt : a text file (.txt) listing the acronyms used in the stereology reading grids under QuPath, as well as their meaning


Macro_BOS_read_me.txt : a text file (.txt) listing the acronyms used in the Macro_BOS.xlsx file, as well as their meaning.

Macro_BOS.csv : Data file (.csv) containing measurements of macroscopic parameters for all 68 fish sampled during this study. The information contained in this table is as follows: 



Fish_id: identification of the fish. This id is identical to the name given to the pictures of the full ovaries (Macroscopic_pictures_Data)

ICES _Division: International Council for the Exploration of the Sea (ICES) division where the fish was sampled in the Food and agricultural Organization of the United nations (FAO) fishing area 27

ICES_statistical_rectangle : Statistical rectangle where the fish was sampled within the FAO fishing area 27

Date: date the fish was caught (dd/mm/yyyy)

Total_fish_length: total length of the fish (cm)

Ungutted_fish_weight: total weight of the fish (g)

Otolith_ID: unique identification number given to each sampled fish through the Imagine (Ellebode et al., 2022) software used by IFREMER

Parasite: presence (Y) or absence (N) of parasite in or on the fish

age: age (in years) of the fish after analysis of the fish's otolith. The IFREMER laboratory of Boulogne-sur-Mer (FRANCE) executed this analysis

Visual_maturity : visually estimated maturity, after observation macroscopic criteria of the fish's gonad with the naked eye, following the WKASMSF (ICES, 2018) scale

Liver_weight: liver weight (g)

Droite_gonad_weight : gonad weight (g) of right ovary

Gauche_gonad_weight : gonad weight (g) of left ovary

Sections: number of cross sections sampled for the individual



 

Contact :

This dataset was established under the MATO (MATurité Objectif des poissons par l'histologie quantitative) project, during the PhD of Carine Sauger (October 2021-2023), financed by France Fillière Pêche (FFP/2020/AM/MF/109), under the supervision of IFREMER (Institut Français de Recherche pour l'Exploitation de la Mer) and BOREA (Biologie des Organismes et Ecosystèmes Aquatiques), and with the collaboration of a research facility from the University of Caen-Normandie : CMABIO3 (Centre de Microscopie Appliquée à la Biologie). For any enquiries, please contact: carine.sauger@gmail.com or laurent.dubroca@ifremer.fr