DOI: 10.22002/nt1t9-xht78
title: Halobacterium salinarum

description: Archaella and plate
description: <strong>Tilt Series Date:</strong> 2017-05-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Halobacterium salinarum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard H. salinarum medium</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-11/rawdata/</p>Halobacterium_20170525d0003.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-11/rawdata/Halobacterium_20170525d0003.mrc"
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        Halobacterium_20170525d0003_part61_3.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
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        dga2017-05-26-11_slicer18086.png,  Halobacterium archaealla. .   .  A plate-like support (?) just below., 1.1 MB  
        
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        dga2017-05-26-11_slicer18087.png,  Plate absent here, 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-11_slicer18087.png"
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        dga2017-05-26-11_slicer18088.png,  Both the archaella are emanating from top of this structure, 1.5 MB  
        
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        dga2017-05-26-11_slicer18089.png,  Both the archaella are emanating from top of this structure, 1.6 MB  
        
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        dga2017-05-26-11_slicer18090.png, , 1.4 MB  
        
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        dga2017-05-26-11_slicer18091.png, , 1.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-11_slicer18091.png"
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        dga2017-05-26-11_slicer18092.png,  Spread of the curved plate (pink-green), 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-11_slicer18092.png"
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        dga2017-05-26-11_slicer18093.png,  Plate below the archaeal flagella, 1.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-11_slicer18093.png"
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        dga2017-05-26-11_slicer18094.png,  Plate below the archaeal flagella, 1.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-05-26-11_slicer18094.png"
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        dga2017-05-26-11_slicer18095.png,  Plate below the archaeal flagella, 1.9 MB  
        
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