DOI: 10.60507/fk2/pp2mpb
title: Image data related to publication "Developmental programming of Kupffer cells by maternal obesity causes fatty liver disease in the offspring"

description: File List: 

“Maternal Obesity Oil-red-O WT cohort.zip” contains all Oil-red-O (ORO) staining pictures taken with a 40X object. Mice were of C57BL/6 Jrcc WT background.There are in total 6 diet groups indicated with CD meaning control diet, HFD standing for high fat diet. The first diet is the diet of the bearing dam, the second is the diet of the foster mother, and the third is the diet the mice received after weaning. All pictures were used to quantify the lipid content of the liver, shown as “ORO Area (%)” in the figure of the linked publication.

“Maternal Obesity Oil-red-O Hif1a cohort.zip” contains all Oil-red-O (ORO) staining pictures taken with a 40X object. There are in total 4 diet groups indicated with CD meaning control diet, HFD standing for high fat diet. The first diet is the diet of the bearing dam and foster mother, and the second is the diet the mice received after weaning. There are 2 genotypes, WT is Hif1a-f/f; LysM-Cre/+ and KO is Hif1a-f/f; LysM-+/+ litter mate. All pictures were used to quantify the lipid content of the liver, shown as “ORO Area (%)” in the figure of the linked publication.

“Maternal Obesity Oil-red-O Clec4f-DTR cohort.zip” contains all Oil-red-O (ORO) staining pictures taken with a 40X object. All mice were born to HFD-fed dam, fostered by CD-fed mother, and received CD after weaning. There are 2 genotypes, Clec4f-+/+ and Clec4f-DTR/+. All mice received diphtheria toxin at postnatal day (P) 0, which induces apoptosis of Kupffer cells, and Clec4f-+/+ mice and a group Clec4f-DTR/+ mice recieved monocyte/hematopoietic stem and progenitor cell transfer at P1 (indicated as DT + cells). The group of Clec4f-DTR/+ mice receiving DT only is marked with “DT only”. All pictures were used to quantify the lipid content of the liver, shown as “ORO Area (%)” in the figure of the linked publication.

“Hif1a staining postnatal day (P) 0 liver macrophages born to high fat diet (HFD)-fed or control diet (CD)-fed dams.zip” contains immunofluorescence staining pictures of P0 liver macrophages (stained by F4/80) and the Hif1a staining signal within the liver macrophages. Individual pictures are individual cells used to quantify the ratio of Hif1a signal between nuclei and cytoplasm. Cells are either from P0 mice born to HFD-fed or CD-fed dams as indicated on the files.

“Incucyte pictures of hepatocyte lipid accumulation (LD540 signal) cultured with TNFa, ApoE or ApoA1 addition .zip” contain images of hepatocytes and their LD540 signal 2 hours after the addition of TNFa, ApoE or ApoA1. Bright field imaged were used to quantify the amount of hepatocytes. LD540 images were used to quantify the LD540 intensity, i.e. lipid content in hepatocytes. Excel file in the compressed file shows the layout of the 24-well cell culture plate for the experiment.

“Incucyte pictures of hepatocyte lipid droplet formation (LD540 signal accumulation) cultured with Kupffer cells.zip” contain images of hepatocytes and their LD540 signal at base-line (0h) or 3 or 4 hours after the addition of Kupffer cells isolated from CDCDCD or HFDCDCD mice. Bright field imaged were used to quantify the amount of hepatocytes. LD540 images were used to quantify the LD540 intensity, i.e. lipid content in hepatocytes. Excel file in the compressed file shows the layout of the 24-well cell culture plate for the experiment.