DOI: 10.5061/dryad.k0p2ngfgm
title: Medium spiny neuron electrophysiological properties across male rats and female rats in different estrous cycle phases in the nucleus accumbens core: Excitatory synaptic input, action potential, and intrinsic properties

description: This dataset presents electrophysiological properties from whole-cell
 patch clamped nucleus accumbens core medium spiny neurons from male rats
 and female rats recorded in different estrous cycle phases. 
 Excitatory synaptic input, action potential, and intrinsic properties are
 presented in an analyzed format, as well as the original
 electrophysiological recordings of each neuron. These data were originally
 presented across two different papers (Proaño et al., 2018, 2020) and
 further analyzed in an additional paper (Long et al., 2024), all of which
 are cited below. These data were key for establishing that nucleus
 accumbens neuron electrical function changed across the estrous cycle in
 response to its associated hormones, programming a phase-dependent
  sex difference in adulthood. This finding provided a new
 framework for understanding how biological sex and hormone cyclicity
 regulate the neurons implicated in motivated behaviors and related
 functions and disorders.
description: This dataset was collected using whole-cell patch clamp
 recordings of medium spiny neurons in adult male and female rat nucleus
 accumbens core. The recordings were analyzed to generate standard
 attributes describing the biophysical properties of these neurons and were
 compared by sex and female estrous cycle phase. Please see the two
 originating articles for more details: Proano et al., 2018 (https://pubmed.ncbi.nlm.nih.gov/29947588/) and Proano et al., 2020  (https://pubmed.ncbi.nlm.nih.gov/32401164/).
description: ReadMe Document and data spreadsheets finalized 11/22/24  by Armaan Raina
 and John Meitzen, for data originally presented in these publications:
 Proaño SB, Morris HJ, Kunz LM, Dorris DM, Meitzen J. Estrous cycle-induced
 sex differences in medium spiny neuron excitatory synaptic transmission
 and intrinsic excitability in adult rat nucleus accumbens core. J
 Neurophysiol. 2018 Sep 1;120(3):1356-1373. doi: 10.1152/jn.00263.2018.
 Epub 2018 Jun 27. PMID: 29947588; PMCID: PMC6171053. Proaño SB, Krentzel
 AA, Meitzen J. Differential and synergistic roles of 17β-estradiol and
 progesterone in modulating adult female rat nucleus accumbens core medium
 spiny neuron electrophysiology. J Neurophysiol. 2020 Jun
 1;123(6):2390-2405. doi: 10.1152/jn.00157.2020. Epub 2020 May 13. PMID:
 32401164; PMCID: PMC7311720. At the time of reposition data were further
 analyzed in this publication: Long AS, Reich BJ, Staicu AM, Meitzen J. A
 nonparametric test of group distributional differences for hierarchically
 clustered functional data. Biometrics. 2023 Dec;79(4):3778-3791. doi:
 10.1111/biom.13846. Epub 2023 Mar 13. PMID: 36805970; PMCID: PMC10695330.
 The excel spreadsheet features whole-cell patch clamp electrophysiology
 data of nucleus accumbens core medium spiny neuron properties of adult
 male and female rats.  Data were collected in males and in females of
 different estrous cycle phases in females.  Some pieces of data are not
 available and are indicated in the spreadsheet with ‘N/A’. For ‘N/A’
 values in mEPSC of amplitude, frequency, and decay, values are not present
 because these neurons were not recorded in this analysis. For values not
 available in delay to first action potential, greater than two spikes were
 recorded and thus not included in the study.  Please see the cited papers
 for methodological details. There are two types of files associated with
 this repository.  The excel spreadsheet contains the analyzed
 electrophysiological characteristics of all neurons. The name of the excel
 spreadsheet is: Neuron data. The second type of file contains the raw data
 obtained from the whole-cell patch clamp recordings of individual neurons.
 These files are generated from PClamp and have an axon binary format
 (.abf) extension. The files can be read by PClamp, Clampfit, or can be
 accessed through the pyABF, ABFview, AbfConvert, abftools, python or R
 packages/toolkits
 ([https://github.com/topics/abf](https://github.com/topics/abf)). The ABF
 files associated with individual neurons are named starting at Excel file
 sheet 3. File Overview: A)     Neuron data.xlsx This file is an excel
 spreadsheet containing analyzed neuron electrophysiological data. Sheets
 within the file: 1\) Excel file sheet 1 – All Cells: Summary of all
 collected data for electrophysiological properties of medium spiny neurons
 obtained from adult male and female rat nucleus accumbens core. 2\) Excel
 file sheet 2 – Analysis Template: Blank template used to analyze data
 collected from individual medium spiny neurons. 3\) Excel file sheets
 3-End -  Starting at Excel file sheet 3 are sheets dedicated to the
 analysis of recordings from each individual Medium Spiny Neuron for this
 project.  Each tab is named by the date (MMDDYY) plus N and a number
 indicating the number of the cell recorded on that date. For example,
 040419N1 would be the first cell recorded on 04/04/19, whereas 040419N2
 would be the second cell recorded on 04/04/19.  B) .abf Files: There are
 1483 .abf files associated with this project.  Data are organized into two
 zipped folders, one containing mEPSC data and the other containing
 additional electrophysiological properties. Within the zipped folder,
 individual folders are named by the date of recording: take folder 040419
 as an example (Cells recorded on 04/04/19). Within each date folder, is a
 folder dedicated to an individual neuron recorded on that date, in the
 040419 folder are 040419N1, 040419N2, …, 040419N6 (Each folder contains
 the files for the respective recording). The folders for each individual
 neuron are named as stated on the “Neuron data” excel spreadsheet.  Within
 each of these folders are the .abf files associated with the recordings of
 that individual neuron.  The identity of the .abf files used for analyzing
 specific neuron electrophysiological properties are defined on the
 respective excel sheet. Version Changes: 11/22/24: Added abf files
 "neuron_electrophysiology_mEPSC_abf_files.zip" which contain the
 mEPSC data. Updated Excel file sheet to include names of files containing
 mEPSC data for each respective recorded neuron.