DOI: 10.5061/dryad.37pvmcvmc
title: Data from: Virus infection and host plant suitability affect feeding behaviors of cannabis aphid (Hemiptera: Aphididae), a newly described vector of potato virus Y

description: Aphids are the most prolific vectors of plant viruses resulting in
 significant yield losses to crops worldwide. Potato virus Y (PVY) is
 transmitted in a non-persistent manner by 65 species of aphids. With the
 increasing acreage of hemp (Cannabis sativa L.) (Rosales: Cannabaceae) in
 the U.S, we were interested to know if the cannabis aphid (Phorodon
 cannabis Passerini) (Hemiptera: Aphididae) is a potential vector of PVY.
 Here, we conduct transmission assays and utilize the electrical
 penetration graph (EPG) technique to determine whether cannabis aphids can
 transmit PVY to hemp (host) and potato (non-host) (Solanum tuberosum L.)
 (Solanales: Solanaceace). We show for the first time that the cannabis
 aphid is an efficient vector of PVY to hemp (96%) and potato (91%) using
 cohorts of aphids. In contrast, individual aphids transmitted the virus
 more efficiently to hemp (63%) compared to potato (19%). During the
 initial 15 minutes of EPG recordings, aphids demonstrated lower number and
 time spent performing intracellular punctures on potato compared to hemp,
 which may in part explain low virus transmission to potato using
 individual aphids. During the entire 8-hour recording, viruliferous aphids
 spent less time ingesting phloem compared to non-viruliferous aphids on
 hemp. This reduced host suitability could potentially cause aphids to
 disperse to more suitable hosts thereby increasing virus transmission.
 Overall, our study shows that cannabis aphid is an efficient vector of
 PVY, and that virus infection and host plant suitability affect feeding
 behaviors of the cannabis aphid in ways which may increase virus
 transmission.
description: This dataset was collected from electrical penetration graph
 (EPG) analysis experiments which were utilized to observe differences in
 feeding behaviors of cannabis aphids which were subjected to four
 treatments. The treatments were chosen in order to observe effects of
 virus presence in the aphid and host plant suitability on feeding
 behaviors of the cannabis aphid. The treatments were 1) non-viruliferous
 aphid probing on hemp, 2) non-viruliferous aphid probing on potato,
 3) viruliferous aphid probing on hemp, 4) viruliferous aphid probing on
 potato. This dataset includes grids (.ana files) obtained from analysis of
 recorded EPG waveforms in Stylet software; R files containing statistical
 analysis between treatments; R files containing construction of figures;
 .csv files containing the outputs from the EPG excel macro worksheet
 developed by Sarria et al. (2009). Three different time periods of the EPG
 recording were analyzed (initial 15 minutes, initial 30 minutes, and
 entire 8 hours). This dataset was also collected from
 individual transmission assays (using one cannabis aphid per plant) and
 cohort transmission assays (using groups of 20 cannabis aphids per plant).
 Data for both individual and cohort assays are contained as separate .xlsx
 files. Additionally, this dataset includes data from a
 population growth experiment utilizing cannabis aphids and potato plants
 to confirm potato as a non-host for the cannabis aphid. This data is
 presented in supplementary_table_1.xlsx.
description: There are not R files associated with the treatment of
 viruliferous aphid probing on potato because there were not enough
 replicates to allow for statistical analysis. However, the .csv files and
 .ana grids have been included in this dataset. The reason for different
 sample sizes in different time periods of the same treatments (i.e.
 15 minutes non-viruliferous aphid probing on hemp n=21 and 8 hours
 non-viruliferous aphid probing on hemp n=23) is because analysis was only
 performed on aphids which probed, and some aphids made their first probe
 after the initial 15 minutes had passed, so they were excluded from the 15
 minute analysis.