DOI: 10.22002/sh131-t8m26
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-45/3dimage_69090/</p>12jan20_C110_3_pNL_INFalpha_man0002_SIRT.rec,  Reconstruction (Pixel Size 2.0 nm), 2.5 GB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 557.4 MB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_6.tiff,  file associated to 3D Image #69090, 826.0 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_5.tiff,  file associated to 3D Image #69090, 571.7 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_4.tiff,  file associated to 3D Image #69090, 593.7 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_3.tiff,  file associated to 3D Image #69090, 554.9 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_2.tiff,  file associated to 3D Image #69090, 590.1 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_1a.tiff,  file associated to 3D Image #69090, 154.7 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_SIRT_1.tiff,  file associated to 3D Image #69090, 741.9 kB  
        
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        12jan20_C110_3_pNL_INFalpha_man0002_full.tiff,  file associated to 3D Image #69089, 1.2 MB  
        
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