cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25345/c5vx3q
title: MassIVE MSV000085613 - Glycoproteomic Characterization of Human High-Grade Serous Ovarian Cancer
title: Inter-tumor heterogeneity is a reflective of the coalescence of a myriad of genomic, transcriptional, translational, and post-translational molecular features. To investigate the contribution of protein glycosylation in tumor heterogeneity in high-grade serous ovarian carcinoma (HGSC), we performed mass-spectrometry (MS)-based glycoproteomic characterization of 119 TCGA HGSC tissues. These HGSC tumor tissues have been previously characterized at the genomic and transcriptomic levels, as well as the proteomic and phosphoproteomic levels, by The Cancer Genomic Atlas (TCGA) consortia and the Clinical Proteomic Tumor Analysis Consortium (CPTAC), respectively. Employing two glycoproteomic strategies, Solid-Phase Extraction of Glycosite-containing peptides (SPEG) for glycosite analysis, and intact glycopeptides for investigation of glycosite-specific glycans (IGPs), we profiled the N-linked glycoproteome in HGSCs, identifying and quantifying glycosites and attached glycan structures.
DOI: 10.25345/c5wt8w
title: MassIVE Reanalysis RMSV000000178.1 - Reanalysis of MSV000080846 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5xt9k
title: MassIVE Reanalysis RMSV000000170.1 - Reanalysis of MSV000080812 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c50n1f
title: MassIVE Reanalysis RMSV000000185.1 - Reanalysis of MSV000080874 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c53h78
title: MassIVE MSV000085619 - Streptomyces clavuligerus Tahlan Lab
title: Metabolomics of S. clavuligerus and some engineered strains
DOI: 10.25345/c54b2z
title: MassIVE Reanalysis RMSV000000184.1 - Reanalysis of MSV000080700 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5772v
title: MassIVE MSV000085618 - Lupine rhizosphere culture collection - Streptomyces sp. S29 coculture dataset
title: Desferrioxamine rich sec-butanol fractions from Streptomyces sp. S29 (Atacama Desert) cocultured with A. niger and B. cinerea. (ED24-26) S29 + A. niger, (ED36-38) S29 + B. cinerea, (ED103-105) S29 monoculture. SJ-123 is a ISP2 medium blank and 'BLANK' is an instrument blank.
DOI: 10.25345/c58458
title: MassIVE Reanalysis RMSV000000183.1 - Reanalysis of MSV000080731 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5c13d
title: MassIVE MSV000085617 - GNPS_Enniatins_and_Beauvericins_from_Fungal_cultures
title: This data set comprises MS2 files obtained from crude extract of fungal cultures as well as purified beauvericin and enniatins A, A1, B, B1, B4 and F.
DOI: 10.25345/c5ct87
title: MassIVE Reanalysis RMSV000000182.1 - Reanalysis of MSV000080788 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5gn0d
title: MassIVE Reanalysis RMSV000000189.1 - Reanalysis of MSV000080803 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5gq6c
title: MassIVE MSV000085616 - Changes in the ECM during mouse kidney development
title: Wildtype C57BL/6 mice kidneys from E14.5, E18.5, P3, and adult were isolated. Proteins were sequentially fractionated and the CS and IN fractions were processed and analyzed using mass spectrometry.
DOI: 10.25345/c5hn0q
title: MassIVE Reanalysis RMSV000000181.1 - Reanalysis of MSV000080827 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5mh7x
title: MassIVE MSV000085615 - GNPS_Enniatins_and_Beauvericins_from_Fungal_culture
title: This data set comprises MS2 files for the crude extract of several fungal species and purfied beauvericin and enniatins A, A1, B, B1, B4, G and F.
DOI: 10.25345/c5nb2m
title: MassIVE Reanalysis RMSV000000180.1 - Reanalysis of MSV000080848 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5r447
title: MassIVE Reanalysis RMSV000000187.1 - Reanalysis of MSV000080770 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5r72h
title: MassIVE MSV000085614 - Tandem MS-MS data of colipterins produced by Escherichia coli Nissle1917
title: LC-MS/MS data set of colipterin metabolites produced by Escherichia coli Nissle1917. These metabolites are stimulated in the presence of sub-lethal concentrations of sulfamethoxazole antibiotic.
DOI: 10.25345/c5vt8k
title: MassIVE Reanalysis RMSV000000186.1 - Reanalysis of MSV000080834 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5zq5b
title: MassIVE MSV000085620 - Hyperlipidemia caused by a missense mutation of A1CF is linked to elevated cellular vesicle transport
title: Xu Y, Stanclift C, Jourdan A, Nagai TH, Yu H, Vellarikkal SK, Deik A, Bullock K, Schenone M, Cowan C, Clish CB, Mootha V, Carr SA, and Kathiresan S. 2020. Recent population genetic studies highlight a missense variant (G398S) of A1CF that is strongly associated with higher levels of blood triglycerides (TG) and total cholesterol (TC). Functional analyses suggest that the A1CF mutation accelerates the secretion of very low-density lipoprotein (VLDL) from the livers. But the underlying mechanism is not clear. Here, we used metabolomics analysis to validate the lipid phenotypes, and proteomics and RNA-seq analyses to interrogate the functional difference between the WT and mutant A1CF.
DOI: 10.25345/c53b31
title: MassIVE Reanalysis RMSV000000192.1 - Reanalysis of MSV000080254 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5744k
title: MassIVE Reanalysis RMSV000000191.1 - Reanalysis of MSV000080765 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5bt99
title: MassIVE Reanalysis RMSV000000190.1 - Reanalysis of MSV000080880 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5kh80
title: MassIVE MSV000085623 - Test Test Test Test Test Test Test
title: htdrhtdhfdsvbsdfvbsdfbsfbgdfgbhdgbgdfgbertbertbdrgbdftgb
DOI: 10.25345/c5mb29
title: MassIVE Reanalysis RMSV000000188.1 - Reanalysis of MSV000080775 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5q44x
title: MassIVE Reanalysis RMSV000000195.1 - Reanalysis of MSV000080750 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5q71t
title: MassIVE MSV000085622 - GNPS Scopalina hapalia fractions
title: Scopalina hapalia from Mayotte, polar fractions. Data acquired using Bruker Impact II and RP-C18 UHPLC Dionex Ultimate 3000 positive mode
DOI: 10.25345/c5tx6j
title: MassIVE Reanalysis RMSV000000194.1 - Reanalysis of MSV000080757 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5v144
title: MassIVE MSV000085621 - GNPS - MS/MS spectra of colipterins produced by E. coli
title: High-resolution tandem MS spectra of colipterin metabolites produced by Escherichia coli. These metabolites are stimulated in the presence of sub-lethal levels of sulfamethoxazole antibiotic.
DOI: 10.25345/c5zm9t
title: MassIVE Reanalysis RMSV000000193.1 - Reanalysis of MSV000080746 - Spectral Library Creation Search - HCD Human
DOI: 10.26066/rds.35990
title: Wheat Sown Areas in Afghanistan 2017

description: Wheat sown areas in Afghanistan for the year 2017. The estimation is performed by harnessing the power of time series sentinel 1 and 2 (Optical and SAR datasets) from November 2016 to August 2017. Phenology based thershold approach along with machine learing classifier is utlized for estimating wheat areas. The validation is performed by calculating confusion matrix, overall accuracy, user accuracy, producers accuracy, Kappa statistics. Total of 4678 ground control points is utlizing from wheat, orchards, vegetables, vineyards, poppy etc. in the accuracy assessment process.
DOI: 10.25345/c52f3r
title: MassIVE Reanalysis RMSV000000200.1 - Reanalysis of MSV000080620 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c56448
title: MassIVE Reanalysis RMSV000000199.1 - Reanalysis of MSV000080744 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c59x6w
title: MassIVE Reanalysis RMSV000000198.1 - Reanalysis of MSV000080741 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5fn03
title: MassIVE Reanalysis RMSV000000197.1 - Reanalysis of MSV000079841 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5jf2q
title: MassIVE Reanalysis RMSV000000204.1 - Reanalysis of MSV000080867 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5kf21
title: MassIVE Reanalysis RMSV000000196.1 - Reanalysis of MSV000080854 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5p450
title: MassIVE Reanalysis RMSV000000203.1 - Reanalysis of MSV000080862 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5sx7m
title: MassIVE Reanalysis RMSV000000202.1 - Reanalysis of MSV000080869 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5xn0f
title: MassIVE Reanalysis RMSV000000201.1 - Reanalysis of MSV000080747 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c51f22
title: MassIVE Reanalysis RMSV000000208.1 - Reanalysis of MSV000080763 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5545b
title: MassIVE Reanalysis RMSV000000207.1 - Reanalysis of MSV000080715 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c58x6k
title: MassIVE Reanalysis RMSV000000206.1 - Reanalysis of MSV000080643 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5cq9k
title: MassIVE Reanalysis RMSV000000213.1 - Reanalysis of MSV000080828 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5dn15
title: MassIVE Reanalysis RMSV000000205.1 - Reanalysis of MSV000080791 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5n74b
title: MassIVE Reanalysis RMSV000000211.1 - Reanalysis of MSV000080712 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5rx6x
title: MassIVE Reanalysis RMSV000000210.1 - Reanalysis of MSV000080844 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5wn04
title: MassIVE Reanalysis RMSV000000209.1 - Reanalysis of MSV000080794 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c50f2r
title: MassIVE Reanalysis RMSV000000216.1 - Reanalysis of MSV000080857 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c54752
title: MassIVE Reanalysis RMSV000000215.1 - Reanalysis of MSV000080851 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c57x68
title: MassIVE Reanalysis RMSV000000214.1 - Reanalysis of MSV000080783 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5bq8w
title: MassIVE Reanalysis RMSV000000221.1 - Reanalysis of MSV000080736 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5gf23
title: MassIVE Reanalysis RMSV000000220.1 - Reanalysis of MSV000080801 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5hf2d
title: MassIVE Reanalysis RMSV000000212.1 - Reanalysis of MSV000080849 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5m741
title: MassIVE Reanalysis RMSV000000219.1 - Reanalysis of MSV000080776 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5qx70
title: MassIVE Reanalysis RMSV000000218.1 - Reanalysis of MSV000080771 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5vq9x
title: MassIVE Reanalysis RMSV000000217.1 - Reanalysis of MSV000080833 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5374c
title: MassIVE Reanalysis RMSV000000223.1 - Reanalysis of MSV000080764 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c56160
title: MassIVE Reanalysis RMSV000000230.1 - Reanalysis of MSV000080694 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c56x7b
title: MassIVE Reanalysis RMSV000000222.1 - Reanalysis of MSV000080688 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c59q9z
title: MassIVE Reanalysis RMSV000000229.1 - Reanalysis of MSV000080709 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5fj1f
title: MassIVE Reanalysis RMSV000000228.1 - Reanalysis of MSV000080842 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5k74q
title: MassIVE Reanalysis RMSV000000227.1 - Reanalysis of MSV000080749 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5q16n
title: MassIVE Reanalysis RMSV000000226.1 - Reanalysis of MSV000080754 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5tq87
title: MassIVE Reanalysis RMSV000000225.1 - Reanalysis of MSV000080695 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5zf2f
title: MassIVE Reanalysis RMSV000000224.1 - Reanalysis of MSV000079562 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c5273p
title: MassIVE Reanalysis RMSV000000231.1 - Reanalysis of MSV000080816 - Spectral Library Creation Search - HCD Human
DOI: 10.25345/c58q9n
title: MassIVE MSV000085647 - Callichilia inaequalis roots alkaloid extract
title: LC-MS/MS data of Callichilia inaequalis roots alkaloid extract DDA TOP3, ESI positive mode
DOI: 10.25345/c5dj0r
title: MassIVE Reanalysis RMSV000000234.3 - Offical Proteogenomic CPTAC Search Results
DOI: 10.25345/c5j74d
title: MassIVE Reanalysis RMSV000000233.2 - MODa Re-analysis of CPTAC Patient 3518
DOI: 10.25345/c5p16b
title: MassIVE Reanalysis RMSV000000232.5 - Reanalysis_Peak_UCSD_Courses_Test.04.27.2017
DOI: 10.25345/c5sq99
title: MassIVE Reanalysis RMSV000000232.4 - MAESTRO Re-analysis of CPTAC Patient 3518
DOI: 10.25345/c5xj1s
title: MassIVE Reanalysis RMSV000000232.1 - ENOSI Re-analysis of CPTAC Patient 3518
DOI: 10.25345/c5516p
title: MassIVE MSV000085648 - JH_multiherb_TCM_Formula_200630_V1
title: Study of a ten-herb TCM formula based on FBMN in both ionization modes. Raw data file from UHPLC-UV-PDA-HRMSMS and UHPLC-UV-PDA-QMS-ELSD. HRMS/MS processed data files from MZmine 2. Cytoscape files grouping all metadata (GNPS libraries, in silico spectral database from DNP).
DOI: 10.25345/c51754
title: MassIVE MSV000085649 - Stem Cell Derived Secretome Renders Therapeutic Potential in Steroid Induced and Genetic Glaucoma Models
title: TCA-precipitated proteins were urea-denatured, reduced, alkylated and digested with endoproteinase Lys-C followed by modified trypsin. Peptide mixtures were loaded onto 250 um fused silica microcapillary columns packed with strong cation exchange resin and 5-um C18 reverse phase, and then connected to a 100 um fused silica microcapillary column packed with 5-um C18 reverse phase. Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ Orbitrap Elite mass spectrometer equipped with a nano-LC electrospray ionization source. Fully automated 10-step MudPIT runs were carried out on the electrosprayed peptides. Tandem mass (MS/MS) spectra were interpreted using ProluCID against a database consisting of 79096 nonredundant Human proteins (NCBI, 2016-06-10 release), 193 usual contaminants. To estimate false discovery rates (FDR)s, the amino acid sequence of each non-redundant proteinentry was randomized to generate a virtual library. This resulted in a total library of 158578 non-redundant sequences against which the spectra were matched. All cysteines were considered as fully carboxamidomethylated, while methionine oxidation was searched as a differential modification. DTASelect and swallow, an in-house developed software, were used to filter ProLuCID search results at given FDRs at the spectrum, peptide, and protein levels. Here all controlled FDRs were less than 5%. All 4 data sets were contrasted against their merged data set, respectively, using Contrast v 1.9 and in house developed sandmartin v0.0.1. Our in-house developed software, NSAF7 v0.0.1, was used to generate spectral count-based label free quantitation results.
DOI: 10.25345/c5cj1t
title: MassIVE MSV000085655 - GNPS L1 and adult metabolome analysis raw data
title: MS/MS analysis of exometabolome of WT and mutant C. elegans
DOI: 10.25345/c5hb3r
title: MassIVE MSV000085653 - THP1 OGlcNAc Enrichment by LWAC
title: O-GlcNAc modified peptide enrichment from human THP1 cells using lectin weak affinity chromatography
DOI: 10.25345/c5n161
title: MassIVE MSV000085652 - Stem Cell Derived Secretome Renders Therapeutic Potential in Steroid Induced and Genetic Glaucoma Models
title: TCA-precipitated proteins were urea-denatured, reduced, alkylated and digested with endoproteinase Lys-C followed by modified trypsin. Peptide mixtures were loaded onto 250 um fused silica microcapillary columns packed with strong cation exchange resin and 5-um C18 reverse phase, and then connected to a 100 um fused silica microcapillary column packed with 5-um C18 reverse phase. Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ Orbitrap Elite mass spectrometer equipped with a nano-LC electrospray ionization source. Fully automated 10-step MudPIT runs were carried out on the electrosprayed peptides. Tandem mass (MS/MS) spectra were interpreted using ProluCID against a database consisting of 79096 nonredundant Human proteins (NCBI, 2016-06-10 release), 193 usual contaminants. To estimate false discovery rates (FDR)s, the amino acid sequence of each non-redundant proteinentry was randomized to generate a virtual library. This resulted in a total library of 158578 non-redundant sequences against which the spectra were matched. All cysteines were considered as fully carboxamidomethylated, while methionine oxidation was searched as a differential modification. DTASelect and swallow, an in-house developed software, were used to filter ProLuCID search results at given FDRs at the spectrum, peptide, and protein levels. Here all controlled FDRs were less than 5%. All 4 data sets were contrasted against their merged data set, respectively, using Contrast v 1.9 and in house developed sandmartin v0.0.1. Our in-house developed software, NSAF7 v0.0.1, was used to generate spectral count-based label free quantitation results.
DOI: 10.25345/c5rt78
title: MassIVE MSV000085651 - GNPS L1 and adult metabolome analysis of C. elegans
title: analysis of exometabolome of C. elegans of mutants and wildtype
DOI: 10.25345/c5wj1g
title: MassIVE MSV000085650 - gender-related secretome of Plasmodium berghei sexual stages
title: Plasmodium, the malaria parasite, undergoes a complex life cycle, which alternates between a vertebrate host and a mosquito vector of the genus Anopheles. In red blood cells of the vertebrate host, Plasmodium multiplies asexually or differentiates into gamete precursors, the male and female gametocytes, responsible for parasite transmission. Sexual stage maturation occurs in the midgut of the mosquito vector, where male and female gametes egress from the host erythrocyte to form a zygote. Gamete egress entails the successive rupture of two membranes surrounding the parasite, the parasitophorous vacuole membrane and the erythrocyte plasma membrane. In this study, we applied a proteomic approach to identify proteins differentially released/secreted by female and male gametocytes of the rodent Plasmodium berghei activated to form gametes. We compared secreted molecules of the wild type gametocytes with those of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive dataset of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules.
DOI: 10.25345/c50b33
title: MassIVE MSV000085662 - Quantitative proteomic comparison of brains from migratory versus resident Junco
title: Quantitative proteomic comparison of brains from migratory versus resident Junco
DOI: 10.25345/c54150
title: MassIVE MSV000085660 - Systematic evaluation and modulation of HLA class I downregulation in Merkel cell carcinoma
title: Viruses avoid immune surveillance through an array of mechanisms, including perturbation of HLA class I (HLA I) antigen presentation. Merkel cell carcinoma (MCC) is a neuroendocrine skin cancer often caused by the Merkel cell polyomavirus (MCPyV) that exhibits low HLA class I surface expression. Through the characterization of 11 newly generated MCC cell lines, we identified multiple class I defects including transcriptional suppression of HLA I genes and NLRC5 alterations. To systematically identify regulators of HLA I loss in MCC, we performed parallel genome-scale gain- and loss-of-function screens in an MCPyV-positive line and identified the noncanonical Polycomb repressive complex PRC1.1 and MYCL as HLA I suppressors. Both hits interact with MCPyV viral antigens, and pharmacologic inhibition of PRC1.1 component USP7 resulted in HLA I upregulation. These findings establish a mechanism for MCPyV-mediated HLA class I suppression and identify therapeutic targets for HLA restoration in MCC.
DOI: 10.25345/c57t80
title: MassIVE MSV000085658 - Neutral endopeptidase-induced chronic diabetic wound model in B6.Cg-lepob/J mice
title: Chronic wounds are a serious and debilitating complication of diabetes. A better understanding of the dysregulated molecular mechanisms at specific stages following injury is crucial for the development of more effective treatments. This study aimed to develop a Neutral endopeptidase (NEP)-induced chronic wound model that mimic the clinical characteristics of diabetic wounds and to subsequently identify dysregulated healing responses. Full thickness excisional wounds were induced on the dorsal surface of obese diabetic (B6.Cg-lepob/J, ob/ob, n=36) (10-12 weeks; 46.1+or-4.2 g) (blood glucose 423,25+or-127,92 mg/dL) and wild-type control (C57BL/6J, n=14) (10-12 weeks; 27.3+or-3.1 g) (blood glucose 186,67+or-24,5 mg/dL) mice. To determine the optimal concentration of NEP required to induce a chronic wound in ob/ob mice, tissue sections (days 0, 2) were analysed for MMP-9 expression (ELISA), Substance P expression (IHC), NEP enzymatic activity (kinetic assay) and the cytokine profile (multiplex bead-array) was determined in wound fluid. The wound healing dynamics (histology: H&E, Massons' Trichrome) as well as the proteomic profile (LC-MS/MS) of acute (WT + saline) and chronic wounds (ob/ob + NEP high) were compared on day 2 and 7 post wounding. Subcutaneous injection of NEP high (1.02 mg/uL; 150 pmol/hour/ug) in the wound edges of ob/ob mice on day 0, reduced Substance P expression (NEP high 9.1+or-5.5% vs NEP low 14.8+or-6.3% wound area, p=0.0064), increased MMP-9 expression (4-8 fold, p=0.0297) and reduced (&gt;2-fold) the cytokine levels (IL1beta, IL6, IL9, IL12p40, IL12p70, IL17, IFNgama, TNFalpha, Eotaxin, G-CSF, GM-CSF, KC, MIP1alpha, MIP1beta, IL3, IL4, IL5, IL10, IL13) within wound fluid 2 days post wounding. Significant wound closure (73.5+or-3.2%), re-epithelization and granulation tissue formation (thickness 1015+or-177 um; cellularity 11.4+or-1.1%) was evident on day 7 in acute wounds (WT + saline) whereas no signs of healing were evident in the NEP-induced chronic wounds. LC-MS/MS identified 906 proteins, of which 23 (Actn3, Itga6, Epb41, Sncg, Nefm, Rsp18, Rsp19, Rpl22, Macroh2a1, Rpn1, Ppib, Snrnp70, Ddx5, Eif3g, Tpt1, FABP5, Cavin1, Stfa1, Stfa3, Cycs, Tkt, Mb, Chmp2a) were differentially expressed on day 2 (p&lt;0.05; &gt;2 fold) and 6 (Cfd, Ptms, Hp, Hmga1, Cbx3, Syap1) (p&lt;0.05; &gt;2 fold) on day 7 post wounding between acute (WT + saline) and chronic wounds (ob/ob +NEP high). The proteomic approach highlighted the complexity of chronic wounds in which the dysregulation involves multiple regulatory pathways and biological processes. A large number of dysregulated proteins in the chronic wounds during the initial stages post wounding was associated with an inability to progress into the proliferative stage of healing and suggest that early intervention might be pivotal for effective healing outcomes. However, given the complexity of dysregulation, therapeutic agents targeting a single pathway is unlikely to be successful.