cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/9c3y4-4ga82
title: HUVECs infx pNLGFPBglI

description: Raw data files of HUVECs infx pNLGFPBglI
description: <strong>Tilt Series Date:</strong> 2013-06-15</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFPBglI obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen 24h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-06-15-11/rawdata/</p>13jun14_C160_2_HUVECspNLBglI24hPI_AT5BT1_0003.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-06-15-11/rawdata/13jun14_C160_2_HUVECspNLBglI24hPI_AT5BT1_0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jun14_C160_2_HUVECspNLBglI24hPI_AT5BT1_0003_part121_15.rec,  Reconstruction (Pixel Size 2.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-06-15-11/3dimage_27480/13jun14_C160_2_HUVECspNLBglI24hPI_AT5BT1_0003_part121_15.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fh5dz-8aj10
title: Crosslinked HIV-1 cores TRIM5a

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5alpha.
description: <strong>Tilt Series Date:</strong> 2013-05-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor and SIRT reconstruction</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-8/3dimage_42959/</p>atlas0008_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-8/3dimage_42959/atlas0008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas0008.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-8/rawdata/atlas0008.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/atk9h-a4z22
title: Vibrio pH 7.5

description: Vibrio cholerae, stationary phase, pH 7.5, 37°C
description: <strong>Tilt Series Date:</strong> 2013-12-18</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MKW1383 grown overnight in LB, pH 7.5 at 37°C</p>
  aba2013-12-18-12_slicer9987.jpg: Vibrio cholerae .  peptidoglycan aba2013-12-18-12_slicer9988.jpg: Vibrio cholerae .  pili aba2013-12-18-12_slicer9989.jpg: Vibrio cholerae .  extracellular vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-18-12/rawdata/</p>VibriopH7setB0006.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-18-12/rawdata/VibriopH7setB0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibriopH7setB0006_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-12-18-12/3dimage_36264/VibriopH7setB0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p9r3m-tdb72
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2012-11-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 82.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-36/rawdata/</p>Bdellovibrio_wt_20121114_0020001.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.4 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        Bdellovibrio_wt_20121114_0020001_part122_2.rec,  Reconstruction (Pixel Size 1.32 nm), 441.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h3535-rw240
title: Alongum

description: Alongum_periplasmiclayers
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-21/rawdata/</p>alongLys_20001.mrc,  Tilt Series (Pixel Size 0.95 nm), 512.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-21/rawdata/alongLys_20001.mrc"
        > <i class="download icon"></i> Download </a></p>

        alongLys_20001_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-21/3dimage_56762/alongLys_20001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j9gt0-keq79
title: Hyphomonas session14 27.5KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-05-22</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, overnight at 4degree...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-46/rawdata/</p>09may22b_bx22gr1_021.mrc,  Tilt Series (Pixel Size 0.774 nm), 520.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-46/rawdata/09may22b_bx22gr1_021.mrc"
        > <i class="download icon"></i> Download </a></p>

        09may22b_bx22gr1_021_1k.rec,  Reconstruction (Pixel Size 1.548 nm), 68.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-46/3dimage_7664/09may22b_bx22gr1_021_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f2xwp-zcf78
title: BsubtilisMJ007 germinating spores

description: Bsubtilis MJ007 germinating spores
description: <strong>Tilt Series Date:</strong> 2011-08-24</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-24-27/3dimage_24985/</p>MJgermspore0007_full.rec,  Reconstruction (Pixel Size 1.9 nm), 908.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-24-27/3dimage_24985/MJgermspore0007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        MJgermspore0007.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-24-27/rawdata/MJgermspore0007.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ntse2-gj887
title: Hyphomonas EB session21 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-07-14</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, EB 0.02mg/ml overnight 4degree...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
  jsa2009-07-14-102_slicer8944.jpg: Hyphomonas neptunium .  DNA treated with EtBr?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-07-14-102/rawdata/</p>09jul14a_b200c_103.mrc,  Tilt Series (Pixel Size 0.632 nm), 511.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-07-14-102/rawdata/09jul14a_b200c_103.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jul14a_b200c_103_part.rec,  Reconstruction (Pixel Size 1.264 nm), 367.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-07-14-102/3dimage_8378/09jul14a_b200c_103_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pmarh-e2x44
title: Hyphomonas session9 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-03-07</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-07-15/rawdata/</p>09mar07a_36c_015.mrc,  Tilt Series (Pixel Size 0.632 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-07-15/rawdata/09mar07a_36c_015.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar07a_36c_015_full.rec,  Reconstruction (Pixel Size 1.264 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-07-15/3dimage_6899/09mar07a_36c_015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ys2waa
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Odonata",
 "Geometry POLYGON ((38.1793 -46.1562, 37.7677 -46.1194, 37.2426 -46.5425, 37.1382 -46.9324, 37.1382 -47.1134, 37.4683 -47.4451, 38.1018 -47.4039, 38.3904 -47.0323, 38.4703 -46.4598, 38.1793 -46.1562))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045461-250811113504898/datasets/export for details.
DOI: 10.22002/sc3ds-rf585
title: MamJ deletion (and might contain

description: Raw data files of MamJ deletion (and might contain
description: <strong>Tilt Series Date:</strong> 2010-02-18</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum</p>
<strong>Strain:</strong> AMB-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR and Peach</p>
<strong>Collaborators and Roles:</strong> Cell culture were from Arash Komeili. Upon receiving, cells were frozen in 2 hours. Meanwhile they were kept in 30C. Data collected and processed by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Stationary phase. </p>
<strong>Sample Preparation:</strong> R2/2, 10nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-02-18-59/rawdata/</p>10feb18a_dJnofe5_059.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-02-18-59/rawdata/10feb18a_dJnofe5_059.mrc"
        > <i class="download icon"></i> Download </a></p>

        10feb18a_dJnofe5_059_full.rec,  Reconstruction (Pixel Size 2.384 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-02-18-59/3dimage_9465/10feb18a_dJnofe5_059_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z3r1y-2qg87
title: HUVECs infx pNLBg1I_endptvirus

description: Raw data files of HUVECs infx pNLBg1I_endptvirus
description: <strong>Tilt Series Date:</strong> 2013-11-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLGFPBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBg1I. Sample was frozen after endpoint of virus infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-53/rawdata/</p>13nov04_Endptvirus_montage_21x21tgt_0012.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-53/rawdata/13nov04_Endptvirus_montage_21x21tgt_0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        13nov04_Endptvirus_montage_21x21tgt_0012_part121_4.rec,  Reconstruction (Pixel Size 2.24 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-53/3dimage_33925/13nov04_Endptvirus_montage_21x21tgt_0012_part121_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a6c7a8
title: Occurrence Download

description: A dataset containing 280 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Arrhamphus sclerolepis)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 280 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045464-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/0p821-n8m61
title: HeLa cells

description: Looking for ESCRT around midbodies
description: <strong>Tilt Series Date:</strong> 2014-11-26</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> Cells provided by Doug Mckarty</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA nup153+Thymidine 2mM for 14 h.</p>
  rra2014-11-26-15_slicer10622.jpg: Midbody stalk
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-15/rawdata/</p>midbody30005.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
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        midbody30005_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
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