cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j07s7mfg
title: CIL:12216, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase in INCENP-OFF cells expressing TrAP-INCENP W766G. Histone H3 modification H3-S10ph shown in green. DAPI (DNA) is blue. See Supplementary Fig 3F panels 5-7 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0ks6qd0
title: CIL:41639, Drosophila melanogaster, ovary. In Cell Image Library

description: A heat map (color intensity look up) of a confocal image of a fruit fly (Drosophila) ovary stained with Dapi. Honorable Mention, 2011 Olympus BioScapes Digital Imaging Competition©.
description: Item type: recorded image; portrayed image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI)
Processing history: heat map indicating intensity
DOI: 10.6075/j08914m4
title: CIL:12219, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase in INCENP-OFF cells expressing TrAP-INCENP WT. Histone H3 modification H3-T3ph shown in red and H3-S28ph in green. DAPI (DNA) is blue. See Supplementary Fig 3E panels 8-11 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0668bvr
title: CIL:12207, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2B panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0kh0m5k
title: CIL:41631, Mus musculus, fibroblast. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label
Visualization methods: CeruleanFP; EGFP; VenusFP; tdTomatoFP; mCherryFP
description: Confocal micrograph of NIH-3T3 cells co-transduced with 5 fluorescent proteins. The cells were marked by co-transduction with Lentiviral Gene Ontology (LeGO) vectors expressing Cerulean (cyan), EGFP (green), Venus (yellow), tdTomato (magenta) or mCherry (red) fluorescent proteins to provide combinatorial colors for cell lineage tracing. These are cytosolic-untargeted FPs (just to mark the cells). The image was collected on live cells with a Leica SP5 confocal system. Honorable Mention, 2011 Olympus BioScapes Digital Imaging Competition©.
DOI: 10.6075/j0k35sdj
title: CIL:12206, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2B panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0ht2n6p
title: CIL:41630, Mus musculus, astrocyte. In Cell Image Library

description: Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
description: Confocal micrograph of a mouse retina showing retinal astrocytes in red and blood vessels in green. Honorable Mention, 2011 Olympus BioScapes Digital Imaging 
 Competition©.
DOI: 10.6075/j04m93dx
title: CIL:41628, Xenopus, melanocyte. In Cell Image Library

description: Fluorescent micrograph of a Xenopus melanophore, showing microtubules (green), microtubule plus ends (red) and nucleus (blue). Honorable Mention, 2011 Olympus 
 BioScapes Digital Imaging Competition©.
description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
DOI: 10.6075/j0m907hr
title: CIL:41623, Asplenium trichomanes, spore. In Cell Image Library

description: A brightfield, multi-focus stack image of a sorus of Asplenium trichomanes (maidenhair spleenwort), a small fern. A sorus is a cluster of sporangia, which produce and contain spores. Honorable Mention, 2011 
 Olympus BioScapes Digital Imaging Competition©.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: bright-field microscopy; multi-focus image
Parameter imaged: absorption of illumination
Source of contrast: differences in intrinsic optical density
DOI: 10.6075/j05q4tz7
title: CIL:41635, Craterium minutum, spore. In Cell Image Library

description: Fluorescent image of the sporangium, an enclosure in which spores are formed, of the slime mold Craterium minutum. Honorable Mention, 2011 Olympus BioScapes Digital Imaging Competition©.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
DOI: 10.6075/j0sq8z64
title: CIL:41402, Paramecium. In Cell Image Library

description: Differential interference contrast image of a Paramecium (unicellular ciliate protozoa) surface. The image shows three water vacuoles contracting. Specimen observed at 100x. Judges' Special Award for Technical Merit, 2010 Olympus BioScapes Digital Imaging Competition©.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
DOI: 10.6075/j0cz35wc
title: CIL:12233, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP WT and treated with ZM44749. Endogenous CENP-E is green and DAPI (DNA) is blue. See Supplementary Fig 5C panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0cf9ntc
title: CIL:12167, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Aurora B localizes to centromeres in early mitosis, but fails to transfer to the spindle at anaphase in wild type clone 18 cells grown in the Aurora B inhibitor ZM447439. Cells were stained for Aurora B (green), plus alpha-tubulin (red) and DAPI for DNA (blue). See Fig 6E panel 5 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0rv0mdq
title: CIL:13999, Homo sapiens. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: single-spot confocal microscopy
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Hoechst 33342; Alexa Fluor 488; Alexa Fluor 568
description: Hela cells were treated with anti-SENP6 siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red, Alexa Flour 568), CenpI antibody (green, Alexa Fluor 488) and Hoechst 33342 (blue).

Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm).

Image Reference: PMID 20212317
DOI: 10.6075/j0vm4b3d
title: CIL:41466, Sus scrofa domestica, epithelial cell. In Cell Image Library

description: Confocal image of a mitotic spindle in a dividing cell. The spindle is shown in yellow and the surrounding actin cytoskeleton is in blue. Images were acquired using a 100X NA 1.4 phase objective lens and a Perkin Elmer spinning disc confocal scan head. A Hammamatsu Orca ER cooled CCD camera was used to collect images. Image collection was driven using Metamorph software. Complete Z-series of images were collected and deconvolved using AutoQuant software. The image is a maximum intensity projection of a subset of the entire Z-stack; the same subset of the Z-stack is used in each channel. Sixth Prize, 2007 Olympus BioScapes Digital Imaging Competition.
description: Preparation: glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody; Fluorescein (FITC); Rhodamine
Processing history: deconvolution; maximum projection
DOI: 10.6075/j0h130xp
title: CIL:41465, Rattus, glial cell, purkinje neuron. In Cell Image Library

description: Photomicrograph of a midsaggital section showing the different components of the rat cerebellum, including Purkinje neurons in green, glia (non-neuronal cells) in red, and cell nuclei in blue.This image is part of an ongoing effort to develop methods to enable the creation large-scale 
 digital atlases of the brain. Fifth Prize, 2007 Olympus BioScapes Digital Imaging.
description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
DOI: 10.6075/j08051f2
title: CIL:41462, diatom. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
description: Star-like colonial diatom imaged with fluorescence microscopy. Green indicates newly depositing silica, red is chlorophyll. Tenth Prize, 2008 Olympus BioScapes Digital Imaging Competition©.
DOI: 10.6075/j05t3j75
title: CIL:12177, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENPOFF cells expressing TrAP-INCENP WT. See Fig 6A panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0df6q2h
title: CIL:41467, Mus musculus, fibroblast. In Cell Image Library

description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Processing history: images stitched together
description: Fluorescent image of serum starved fibroblasts created from four images stitched together. Image shows the microtubules (green) and nucleus (red). Ninth Prize, 2007 Olympus BioScapes Digital Imaging Competition.
DOI: 10.6075/j0542m85
title: CIL:12187, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) to outer kinetochores during mitosis in INCENP OFF cells expressing T TrA_-INCENP (red). DAPI (DNA) is blue. See Fig 8C panels 1-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0k072zr
title: CIL:13372, Homo sapiens. In Cell Image Library

description: Z focal series of Bod-1 depleted HeLa cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). Bod1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. This image is the Bod-1 depletion experiment for the control CIL: 13374.
description: Cells were fixed with 3.7% PFA or in methanol for 2 min at −20°C. Mouse anti–α-tubulin DM1A (Sigma-Aldrich), and mouse anti–Aurora B antibody AIM-1 (BD Biosciences) were used at 1:500. Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Image corresponds to Figure 5A from JCB vol. 179 no. 2 187-197.
description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: constrained iterative deconvolution
DOI: 10.6075/j0pz57nm
title: CIL:41568, Homo sapiens, macrophage. In Cell Image Library

description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
description: Video showing HIV-1‚Äìinfected human macrophage sensing its environment. Images were collected on a spinning disk confocal microscope using a 100X objective. Honorable Mention, 2011 Olympus BioScapes Digital Imaging Competition©.
DOI: 10.6075/j0ht2n3b
title: CIL:12155, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. In inner centromere protein INCENP-OFF cells, levels of phosphorylated histone H3 (H3-S10ph, red, and H3-S28ph, green) are low compared to INCENP-ON cells. DAPI stained chromosomes shown in blue. See Fig 2A panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.15468/dl.7xgadx
title: Occurrence Download

description: A dataset containing 26459 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Europe",
 "Country is Russian Federation",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Heracleum sosnowskyi"
 ]
}

 The dataset includes 26459 records from 28 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009737-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0fb51qn
title: CIL:12181, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) in INCENP OFF cells expressing TrAP-INCENPW766G (red). DAPI (DNA) is blue. See Fig 8D panels 1-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j00r9n43
title: CIL:12250, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. CENP-E(green)is reduced by deoxycycline treatment (INCENP-OFF). Shown is an anaphase cell. Alpha-tubulin is red and DAPI (DNA) is blue. See Supplementary Fig 1B panels 13-16 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0028q8f
title: CIL:12200, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A . Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2C panels 5-8 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed