DOI: 10.5061/dryad.vmcvdnd2j
title: Natural variation in root exudate composition in the genetically structured Arabidopsis thaliana in the Iberian Peninsula

description: Plant root exudates are involved in nutrient acquisition, microbial
 partnerships, and inter-organism signaling. Yet, little is known about the
 genetic and environmental drivers of root exudate variation at large
 geographical scales, which may help understand evolutionary trajectories
 of plants in heterogeneous environments. We quantified natural variation
 in chemical composition of Arabidopsis thaliana root exudates in 105
 Iberian accessions. We identified up to 373 putative compounds using
 ultra-high performance liquid chromatography coupled with mass
 spectrometry. We estimated broad-sense heritability of compounds and
 conducted a genome-wide association (GWA) study. We associated variation
 in root exudates to variation in geographic, environmental, life history,
 and genetic attributes of Iberian accessions. Only 25 of 373 compounds
 exhibited broad-sense heritability values significantly different from
 zero. GWA analysis identified polymorphisms associated to 12 root exudate
 compounds and 26 known genes involved in metabolism, defense, signaling,
 and nutrient transport. The genetic structure influenced root exudate
 composition involving terpenoids. We detected five terpenoids related to
 plant defense significantly varying in mean abundances in two genetic
 clusters. Our study provides first insights into the extent of root
 exudate natural variation at a regional scale depicting a diversified
 evolutionary trajectory among A. thaliana genetic clusters chiefly
 mediated by terpenoid composition.
description: # Natural variation in root exudate composition in the genetically
 structured Arabidopsis thaliana in the Iberian Peninsula
 [https://doi.org/10.5061/dryad.vmcvdnd2j](https://doi.org/10.5061/dryad.vmcvdnd2j) ## Description of the data and file structure Root exudates in 105 Iberian Arabidopsis thaliana accessions. The file has two sheets including features detected with negative and positive ionization modes using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. ### Files and variables #### File: Raw\_root\_exudate\_data\_9sep24.xlsx **Description:** Sheet #1: Data from 'Negative ionization mode' ##### Variables * 1st row: Variable name * 1st column: Accession (accession name; 3-letter code plus number) * 2nd column: Rosette diameter (plant size at sampling; cm) * 3rd column: Number of leaves (plant size at sampling; count) * 4th-1145th columns: Features codes (from N.V1 to N.V1142; absolute peak area of each feature obtained from ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS)) * Note: Each accession has 3-4 replicates **Description:** Sheet #2: Data from 'Positive ionization mode' ##### Variables * 1st row: Variable name * 1st column: Accession (accession name; 3-letter code plus number) * 2nd column: Rosette diameter (plant size at sampling; cm) * 3rd column: Number of leaves (plant size at sampling; count) * 4th-129th columns: Features codes (from P.V1 to P.V126; absolute peak area; absolute peak area of each feature obtained from ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS)) * Note: Each accession has 3-4 replicates