DOI: 10.6084/m9.figshare.12707903
title: Drosophila_plate01_induced

description: COLLECTION TITLE:<br>* 2020_ACC_RP_DrosophilaBloodCells<br><br>ARTICLE (when using these files, please, cite the following article):<br>* A. Szkalisity, F. Piccinini, A. Beleon, T. Balassa, I.G. Varga, E. Migh, C. Molnar, L. Paavolainen, S. Timonen, I. Banerjee, E. Ikonen, Y. Yamauchi, I. Ando, J. Peltonen, V. Pietiäinen, V. Honti, P. Horvath, Regression plane concept for analysing continuous cellular processes with machine learning. Nature Communications, 2021<br><br>KEYWORDS:<br>* High-Throughput Screening, Fluorescence Microscopy, Drosophila Blood Cells, Machine Learning, Differentiation, Hematopoiesis<br><br>CATEGORIES:<br>* Bioinformatics, Biological Techniques<br><br>BIOLOGICAL APPLICATION:<br>* In the blood cell differentiation study, the immune response of Drosophila melanogaster larvae - the differentiation of lamellocytes - were induced by wounding with an insect pin.<br>* The larvae were bled 12 hours after the wounding, and hemocytes were cultured in a well of an 8 well µ-slide (Ibidi, Cat:80826) in Schneider’s medium (Lonza, Cat: 04-351 Q) supplemented with 10% FBS (Gibco, Cat: 10270), 0,01 mg/ml gentamicin (Sigma, Cat: G3632), 0,065 mg/ml penicillin (Sigma, Cat: P7794) and 0,1 mg/ml streptamicin (Sigma, Cat: S6501) at 25 °C.<br>* The transdifferentiation of plasmatocytes into lamellocytes was monitored by cell type specific transgenes (eaterGFP for plasmatocytes, and MSNF9MOmCherry for lamellocytes as described in Anderl et al., Transdifferentiation and proliferation in two distinct hemocyte lineages in Drosophila melanogaster larvae after wasp infection. PLoS Pathogens, 12, 7, e1005746 2016).<br><br>IMAGES:<br>* 15-frame RGB image sequence/field (141 fields), representing the brightfield, mCherry, EGFP channels, with 2 hours gap between subsequent frames. <br>* Images were acquired with an high-content screening microscope (Operetta, Perkin Elmer) with a 60x high-numeric-aperture objective and a digital high resolution 14-bit CCD camera, for a total of 4230 images (2 plates, 2115 images in each).<br>* The image size is 1360x1024 pixels and 8-bit per channel RGB TIFF format.<br>* In this dataset the images are already prepared for being directly analysed in Advanced Cell Classifier (ACC).<br><br>FORMAT OF THE FILES:<br>* The files in this collection follow the Advanced Cell Classifier (ACC) standard.<br><br>HOW TO OPEN THE DATASET IN ACC:<br>* Download a plate ("plate01_induced" or "plate02_control") into a folder (this parent folder is termed ACC Project Folder)<br>* Download the corresponding trained project file ("Test_ACC_Project.mat").<br>* Launch ACC (freely available for download at cellclassifier.org)<br>* Click 'Open project' in the toolbar and select the downloaded trained project file. When you are asked for a new datapath specify your ACC project folder ("plate01_induced" or "plate02_control").<br><br>IMAGING INFO:<br>* These images were generated by Viktor Honti, Istvan Gergely Varga, and Istvan Ando at the Biological Research Centre (BRC), Szeged, Hungary.<br>* Image features were extracted by Peter Horvath at BRC.<br>* Please contact Viktor Honti (viktor.honti@brc.hu) or Peter Horvath (peter.horvath@brc.hu) for more information.<br><br>MAIN CONTACTS:<br>* Viktor Honti, Biological Research Centre (BRC), Szeged, Hungary. Email: viktor.honti@brc.hu<br>* Peter Horvath, Biological Research Centre (BRC), Szeged, Hungary. Email: horvath.peter@brc.hu<br><br>COPYRIGHT:<br>* Copyright (c) 2020, Viktor Honti, Istvan Gergely Varga, Istvan Ando, Peter Horvath<br>* Biological Research Centre (BRC), Szeged, Hungary<br>* All rights reserved.<br>*<br>* Redistribution and use of the material, with or without modification, is provided for academic research purpose only.<br>*<br>* This material is free; you can redistribute it and/or modify it under the terms of the GNU General Public License version 3 (or higher) as published by the Free Software Foundation.<br>* This material is distributed WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.<br>* See the GNU General Public License for more details.