DOI: 10.5061/dryad.0k6djhbfs
title: Data from: Complete mitochondrial genome of <em>Gyrodactylus nigeri</em> (Platyhelminthes: Monogenea)

description: Gyrodactylus nigeri Zhou & Chen, 2024 was merely distributed in
 Yunnan Province, Southwest China; meanwhile, its mitochondrial genome
 remains unclear. This study aims to sequence the mitogenome of G. nigeri
 and clarify its phylogenetic relationship within the Gyrodactylidea. The
 mitogenome of G. nigeri was sequenced using the next-generation sequencing
 (NGS) method, annotated, and analyzed using bioinformatic tools. The
 mitochondrial genome of G. nigeri is 14,903 bp in length, containing 12
 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal
 RNA genes (rRNAs), and two major non-coding regions (NCR: NC1 and NC2).
 The overall A+T content of the mitogenome is 76.6%, a higher content
 compared with all reported mitochondrial genomes of monogeneans. The
 mitogenome of G. nigeri presents a clear bias in nucleotide composition
 with a negative AT-skew and a positive GC-skew. All tRNAs have the typical
 cloverleaf secondary structure except for tRNACys, tRNASer1, and tRNASer2,
 which lack the dihydrouridine (DHU) arm. Furthermore, two different
 repetitive non-coding regions of 88 bp repeats occurred in the NCR regions
 (NC1 and NC2) with a poly-T stretch, two stem-loop structures with obvious
 differences in the first loop, and a G(A)n motif. The gene order is
 identical to the mitochondrial genomes reported from other Gyrodactylus
 species except Gyrodactylus sp. FZ-2021.Co-phylogenetic analyses showed
 divergence patterns of Gyrodactylus correspond to those of their fish
 hosts, and the overall coevolutionary fit between the parasites and hosts
 was consistently significant. Meanwhile, the results supported the sister
 relationship between G. nigeri and Gyrodactylus sp. FY-2015 from the hosts
 within the Nemacheilidae cluster together with high nodal support based on
 12 PCGs sequences and amino acid sequences. Gyrodactylidae forms an
 independent and monophyletic clade within Gyrodactylidea.
description: # Data from: Complete mitochondrial genome of *Gyrodactylus nigeri*
 (Platyhelminthes: Monogenea) Dataset DOI:
 [10.5061/dryad.0k6djhbfs](https://doi.org/10.5061/dryad.0k6djhbfs) ##
 Description of the data and file structure This GenBank flat file
 describes the complete circular mitochondrial genome (14,903 bp) of
 *Gyrodactylus nigeri*. It includes organism details, sequencing and
 assembly information, and full annotation of genes. The genome contains
 typical mitochondrial protein-coding genes (e.g., **cox**, **nad**,
 **cob**, **atp6**), along with 22 tRNAs and 2 rRNAs. The ORIGIN section
 provides the complete nucleotide sequence of the genome. ### Files and
 variables #### File: Gyrodactylus_nigeri_mitochondrion.gb
 **Description:** The mitogenome sequencing and assembly were performed by
 Sangon Biotech (Shanghai, China). Next-generation sequencing was used to
 sequence the entire mitogenome of *G. nigeri* from a single individual. *
 A 14,903 bp circular mitochondrial genome * From the parasite
 *Gyrodactylus nigeri* * Contains: * 12–13 protein-coding genes typical of
 metazoan mitochondria * 22 tRNA genes * 2 rRNA genes * Assembled using
 Illumina sequencing ## Code/software ``` The mitogenome was assembled
 using GetOrganelle v1.7.7.1 ``` ## Access information Other publicly
 accessible locations of the data: * None Data was derived from the
 following sources: * The complete mitochondrial genome of *Gyrodactylus
 nigeri* was deposited to the GenBank of NCBI
 ([https://www.ncbi.nlm.nih.gov](https://www.ncbi.nlm.nih.gov)) and
 obtained the accession number (GenBank accession no. PX562927).