cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/8wtaf-56191
title: JPA 1558 LMEM

description: JPA 1558 TlpT - YFPlabelled cytoplasmic receptor correlative cryo-LMECT experiment (LM from 051512)
description: <strong>Tilt Series Date:</strong> 2012-05-16</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -12.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Sux media at 30 degrees, Ampicillin treated for one hour (2.57ml sux plus 0.0113g Ampicillin)membrane dye FM64X added 10 min at room temp before antibiotic treatment for one hour</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-5/rawdata/</p>1563_LMEM1.mrc,  Tilt Series (Pixel Size 0.78 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-5/rawdata/1563_LMEM1.mrc"
        > <i class="download icon"></i> Download </a></p>

        1563_LMEM1_part122_18.rec,  Reconstruction (Pixel Size 1.56 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-5/3dimage_18637/1563_LMEM1_part122_18.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c5n3m-8xe36
title: CheA-Ge8-L2

description: This strain lacks a chromosomal CheA, and carries plasmid pj207 containing a CheA with a trimer of the silver peptide in linker region  two.
description: <strong>Tilt Series Date:</strong> 2012-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Dan Feldheim and Carly Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB plus Kanamycin, induced with 14nM IPTG for one hour, penicillin treated for an additional hour</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-9/rawdata/</p>CheAmutantmanual5.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-9/rawdata/CheAmutantmanual5.mrc"
        > <i class="download icon"></i> Download </a></p>

        CheAmutantmanual5_part128_28.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-9/3dimage_17934/CheAmutantmanual5_part128_28.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ztwwx-phn47
title: Rhodopseudomonas palustris TIE-1

description: Raw data files of Rhodopseudomonas palustris TIE-1
description: <strong>Tilt Series Date:</strong> 2012-05-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Rhodopseudomonas palustris</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Nicholas ShikumaDianne Newman</p>
<strong>Purification / Growth Conditions / Treatment:</strong> wild type and shc mutantgrown by photoheterotrophic anaerobic exponential and stationary</p>
<strong>Sample Preparation:</strong> vitrobot</p>
  amw2012-05-09-11_slicer4841.jpg: Intracellular vesicle .  Granules .  Parallel membrane sheet
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-11/rawdata/</p>WT_expo0008.mrc,  Tilt Series (Pixel Size 0.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-11/rawdata/WT_expo0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        WT_expo0008_part122_4.rec,  Reconstruction (Pixel Size 1.8 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-11/3dimage_18470/WT_expo0008_part122_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bgcbk-8em84
title: Agro pJW58

description: wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter.  Kanamycin-resistant (150 to 300 ug/mL)
description: <strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-3/rawdata/</p>Agro_pJW580003.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-3/rawdata/Agro_pJW580003.mrc"
        > <i class="download icon"></i> Download </a></p>

        Agro_pJW580003_part121_2.rec,  Reconstruction (Pixel Size 1.6 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-3/3dimage_39679/Agro_pJW580003_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jtksf-z0n06
title: Vibrio deltaCheA2

description: V. cholerae strain deleted for CheA2
description: <strong>Tilt Series Date:</strong> 2014-04-10</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Ikuro Kawagishi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C</p>
  aba2014-04-10-16_slicer3831.jpg: short polar array in deltaCHeA2 strain
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-04-10-16/rawdata/</p>VibrioDeltaA20014.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-04-10-16/rawdata/VibrioDeltaA20014.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioDeltaA20014_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-04-10-16/3dimage_41257/VibrioDeltaA20014_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r490j-c5842
title: tar-CF-EEEE on vesicles

description: Tar-CF, EEEE, vesicles
description: <strong>Tilt Series Date:</strong> 2014-01-02</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Lynmarie Thompson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Tar-CF-EEEE assembled on vesicles</p>
  aba2014-01-02-10_slicer9533.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-02-10/rawdata/</p>Vesicle4E02Jan0012.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-02-10/rawdata/Vesicle4E02Jan0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        Vesicle4E02Jan0012_part121_14.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-02-10/3dimage_37379/Vesicle4E02Jan0012_part121_14.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cr93s-x8s38
title: UU 2803- A413T delta P1P2

description: E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB medium, lysed with penicillin for 1 hour</p>
<strong>Sample Preparation:</strong> plunge frozen in R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-8/rawdata/</p>2803atlas10008.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-8/rawdata/2803atlas10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        2803atlas10008_part130_5.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-8/3dimage_20515/2803atlas10008_part130_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.angqyt
title: Occurrence Download

description: A dataset containing 962856 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Collomia biflora, Colocasia esculenta, Colubrina asiatica, Colutea arborescens, Combretum indicum, Commelina benghalensis, Commelina communis, Commelina diffusa, Commelina obliqua, Conicosia pugioniformis, Conium maculatum, Conocarpus erectus, Conringia orientalis, Consolida orientalis, Convallaria majalis, Convolvulus arvensis, Conyza bonariensis, Corchorus aestuans, Corchorus fascicularis, Corchorus hirtus)",
 "BasisOfRecord is one of (Machine Observation, Human Observation, Observation)",
 "HasCoordinate is true",
 "Year is greater than or equal to 1990"
 ]
}

 The dataset includes 962856 records from 3281 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001263-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/p55bb-bjq86
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-80/rawdata/</p>RCC745_RELtovis_087.mrc,  Tilt Series (Pixel Size 1.26 nm), 570.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-80/rawdata/RCC745_RELtovis_087.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_087.rec,  Reconstruction (Pixel Size 1.26 nm), 622.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-80/3dimage_12501/RCC745_RELtovis_087.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a8b2wj
title: Occurrence Download

description: A dataset containing 33 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Material sample, Specimen)",
 "OccurrenceStatus is Present",
 "TaxonKey is Heliococcus summervillei"
 ]
}

 The dataset includes 33 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005929-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/vw8zm-y1j82
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-03-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-10-15_slicer7248.jpg: filaments
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-15/rawdata/</p>20140310_YWC092_10015.mrc,  Tilt Series (Pixel Size 3.9 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-10-15/rawdata/20140310_YWC092_10015.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140310_YWC092_10015_part131_7.rec,  Reconstruction (Pixel Size 15.6 nm), 712.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0w3gv-kc783
title: Chromogranin A-GFP

description: CLEM of chromogranin A-GFP expressing INS-1E cells
description: <strong>Tilt Series Date:</strong> 2014-08-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Chromogranin A GFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 68.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Zach Friedberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both side pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads added to grids before freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-15/rawdata/</p>tomogram15.mrc,  Tilt Series (Pixel Size 0.96 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-15/rawdata/tomogram15.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomogram15.rec,  Reconstruction (Pixel Size 3.84 nm), 443.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-15/3dimage_45785/tomogram15.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x7a2p-ye123
title: Hippocampal Neurons (cultured)

description: Plunge frozen at DIV 14
description: <strong>Tilt Series Date:</strong> 2014-10-18</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-10-18-10/rawdata/</p>101814_hipp_neuron_27kx0011.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-10-18-10/rawdata/101814_hipp_neuron_27kx0011.mrc"
        > <i class="download icon"></i> Download </a></p>

        101814_hipp_neuron_27kx0011_full.rec,  Reconstruction (Pixel Size 1.64 nm), 534.5 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qsqv1-85x08
title: Myxococcus xanthus delta-pilQ

description: grid box YWC061
description: <strong>Tilt Series Date:</strong> 2013-12-30</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-93/rawdata/</p>20131230_Janelia_YWC061_10031.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        20131230_Janelia_YWC061_10031_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
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