cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j00p0xrc
title: CIL:11845, Potorous tridactylus, epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation
description: Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is reduced. Retrograde flow in the lamellum is almost stopped. The cell was microinjected with X-rhodamine-conjugated actin and observed by spinning disk confocal microscopy. Fluorescent speckle time-lapse series were acquired on a spinning disk confocal microscope with a 100/1.4 NA Plan Apo objective lens. Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 5B of associated manuscript (J Cell Biol, 161:845-851, 2003).
DOI: 10.6075/j04b3025
title: CIL:11844, Potorous tridactylus, epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation
description: Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell. Fast retrograde flow occurs in the lamellipodium and slow retrograde flow in the lamellum. The cell was microinjected with X-rhodamine-conjugated actin and observed by spinning disk confocal microscopy. Fluorescent speckle time-lapse series were acquired on a spinning disk confocal microscope with a 100/1.4 NA Plan Apo objective lens. Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 5B of associated manuscript (J Cell Biol, 161:845-851, 2003).
DOI: 10.6075/j0s75fsk
title: CIL: 51784, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library

description: A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding drug slowly in 1/4 increments over 90 minutes.
description: ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C

Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files:
1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html)
2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/)
3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory
4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK"
5. The 16 bit image will typically look dark and will require brightness adjustment.
6. In the ImageJ toolbar, select Image &gt;&gt; Adjust &gt;&gt; Brightness/Contrast and select "Auto", or manually set the brightness and contrast.
description: Imaging mode: DICM
Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective
DOI: 10.6075/j0dn4543
title: CIL:10263, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
description: Early stages of dendritic development and synapse formation in cultured hippocampal neurons. This multilayer image shows neurons fixed at 7 days in vitro and immunostained for the dendritically localized protein MAP2 (green) and the presynaptic vesicle protein Synapsin I (red). Unstained axons in the field are evident in the phase channel, which is hidden but can be turned on in the viewer. Neurons at 3, 5 and 7 days in vitro are represented in this image group. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, warmed to 37°C prior to fixation, 15 minutes), permeabilized with 0.25% Triton (7 minutes) and immunostained for MAP2 (monoclonal HM2, Sigma, with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and synapsin I (from P. DeCamilli, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]). Images were acquired with a Leica DMRA microscope with a mercury arc lamp, a 40X lens (HCX PL Fluotar, NA 0.75), Leica GFP filter set (excitation, BP 470/40; dichromatic mirror, 500; suppression filter, BP 525/50); Leica N3 filter set (excitation, BP546/12; dichromatic mirror, 565, suppression filter, BP 600/40), Photometrics CoolSnap ES CCD camera and MetaMorph software.
DOI: 10.6075/j0np235g
title: CIL:11839, Potorous tridactylus, epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation
description: Microtubule fragments exhibiting net treadmilling in a Rac1(Q61L)-expressing PtK1 cell. Microtubule plus ends undergo dynamic instability while their minus ends depolymerize, resulting in net treadmilling of the microtubule lattice. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). For still images, see Fig. 2 D of associated paper (J Cell Biol, 161:845-851, 2003)
DOI: 10.6075/j00z722g
title: CIL:11837, Potorous tridactylus, epithelial cell. In Cell Image Library

description: Microtubules in a dominant-negative Rac1(T17N)-expressing PtK1 cell. Microtubules do not undergo retrograde flow, and microtubules rapidly switch between growth and shortening phases with little net change in length over time. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(T17N) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 3 of associated paper (J Cell Biol, 161:845-851, 2003).
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation
DOI: 10.6075/j0c53jmd
title: CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation
description: Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and remain close to the edge. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(Q61L) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 2 of associated paper (J Cell Biol, 161:845-851, 2003).
DOI: 10.6075/j0zc81pb
title: CIL:11050, Cavia porcellus, spermatocyte. In Cell Image Library

description: See: Fawcett and Chemes (1979) Tissue Cell 11:147-162
description: Transmission electron micrograph of surface of nuclear envelope of guinea pig spermatocyte prepared by the Freeze-fracture technique shows the unusual clustering of nuclear pores in these cells. From Fawcett and Chemes (1979) Tissue Cell 11:147-162, and reprinted with permission as Figure 150 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: replica of fracture surface
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j0g15zn4
title: CIL:11039, Cavia porcellus, Cricetulus griseus, Sertoli cell. In Cell Image Library

description: The micrographs, courtesy of David Phillips, appear as Figures 138 (upper) and 139 (lower) from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Transmission electron micrographs showing the unusual configuration of nucleoli in Sertoli cells, with smaller, highly dense spherical bodies flanking a large central body. Upper micrograph from guinea pig; lower from Chinese hamster.
DOI: 10.6075/j0zp44x9
title: CIL:11027, Didelphimorphia, spermatogonium. In Cell Image Library

description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Transmission electron micrographs of Opossum spermatogonia showing different regions within the nucleolus. One region is more lightly staining and fine textured, the other denser and more coarsely textured.
description: These images are figures 132 (upper) and 133 (lower) from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0jm28fh
title: CIL:11021, Homo sapiens. In Cell Image Library

description: Transmission electron micrograph of isolated HeLa cell metaphase chromosome decondensed by removal of histones with dextran sulfate and heparin, centrifuged onto a carbon film and stained with uranyl acetate. The residual chromosome 'scaffold' is surrounded by extended free DNA loops 20 to 24 micrometers long. Area boxed is shown at higher magnification in the grouped CIL image 11023.
description: Micrograph from J.R. Paulson and U.K. Laemmli (1977) Cell, 12:817-828, and republished with permission as Figure 130 from Chapter 4 (Nucleus) of 'The Cell' by Don W. Fawcett M.D.
description: Preparation: unfixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j0c53jp9
title: CIL:11048, Cavia porcellus, Rattus rattus, Sertoli cell, pancreatic A cell. In Cell Image Library

description: Preparation: cryofixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: See: Fawcett and Chemes (1979) Tissue Cell 11:147-162.
description: Nuclear pores are clearly seen in these transmission electron micrographs of cells after cryo-fixation, freeze-fracture and surface replication. Upper panel shows the outer surface of of the nuclear envelope from a guinea pig Sertoli cell. Lower panel shows the inner surface of the nuclear envelope of of a rat pancreatic acinar cell. Upper micrograph from Fawcett and Chemes (1979) Tissue Cell 11:147-162, reprinted as Figure 148 with permission; lower micrograph courtesy of Bernard Gilula is Figure 149 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0nc5zxq
title: CIL:11041, Barbus tauricus, Homo sapiens, Sertoli cell, glandular cell of endometrium. In Cell Image Library

description: Transmission electron micrographs showing the unusual nucleolar structures in Sertoli cells of ruminants, with varying numbers of vesicles and tubules; the upper panel illustrates such an organization in the domestic bull, Bos taurus. Lower panels show the unusual nucleolar structures present in human endothelium, where a similar system of convoluted tubules is detected during the secretory phase of the menstrual cycle.
description: Upper panel is Figure 140; Lower panels appeared in Tersakis (1965) J. Cell Biol. 27: 158-61, reprinted with permission as Figures 141-3, from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j0rx99xt
title: CIL:11058, Amphiuma tridactylum, epithelial cell, intestinal epithelial cell. In Cell Image Library

description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Transmission electron micrograph of the dense fibrous nuclear lamina (here labeled 'nuclear sheath')closely adpressed to the inner membrane of the nuclear envelope(arrow)in an intestinal epithelial cell of the salamander Amphiuma tridactylum. Compact heterochromatin is seen lining the inner nuclear envelope and nuclear lamina. Figure 156 from Chapter 4 (Nucleus) of 'The Cell' by Don W. Fawcett M.D.
DOI: 10.6075/j02j69n3
title: CIL:40484, Homo sapiens, epidermoid carcinoma. In Cell Image Library

description: Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw
description: Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA031801 (shown in green). HPA031801 is a polyclonal Anti-RPS25 antibody that stains the cytoplasm. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).
description: The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met:

The publication and/or presentation are solely for informational and non-commercial purposes.

The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
DOI: 10.6075/j0t43rw5
title: CIL:11037, Sus scrofa scrofa, Leydig cell. In Cell Image Library

description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Transmission electron micrograph of Leydig cell nucleus from the domestic boar contains a region (arrow) thought to represent the single X chromosome which is largely heterochromatic. The chromosome is closely associated with the prominent nucleolus. Figure 137 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j00r9n60
title: CIL:11046, Cavia sp., erythroblast. In Cell Image Library

description: Transmission electron micrographs showing details of nuclear pores in thin sections of an erythroblast (above) and endothelial cell (below). Pores appear to be filled with dark staining material in these thin sections. Accumulations of heterochromatin at the nuclear envelope are excluded from pore-containing regions.
Figures 146 and 147 from Chapter 4 (Nucleus) of 'The Cell' by Don W. Fawcett M.D.
description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j02f7m7s
title: CIL:11023, Homo sapiens. In Cell Image Library

description: Transmission electron micrograph showing part of a decondensed mitotic chromosome. The residual protein 'scaffold' (at bottom) is surrounded by a 'halo' of DNA loops 20 to 24 micrometers long. The entire chromosome is seen in the grouped CIL image 11021.
description: Micrograph from J.R. Paulson and U.K. Laemmli (1977) Cell, 12:817-828, and republished with permission as Figure 131 from Chapter 4 (Nucleus) of 'The Cell' by Don W. Fawcett M.D. This image is the enlarged inset panel from CIL:11021 in this image group.
description: Preparation: unfixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j0t72g70
title: CIL:11059, Homo sapiens, Rodentia, epidermal cell, Leydig cell. In Cell Image Library

description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Upper panel shows a transmission electron micrograph featuring the nuclear envelope with the nuclear lamina seen here as a uniform layer between the inner nuclear membrane and more darkly staining blocks of heterochromatin in this human skin cell. The lamina appears to be discontinuous over the nuclear pore. Lower panel shows a thinner and more darkly staining lamina in a rodent Leydig cell. Figures 157 (upper, courtesy of George Szabo) and 158 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.