DOI: 10.5061/dryad.k53g5
title: Data from: Hybridisation in closely related Rhododendron species: half of all species differentiating markers experience serious transmission ratio distortion

description: An increasing number of studies of hybridization in recent years have
 revealed that complete reproductive isolation between species is
 frequently not finalized in more or less closely related organisms. Most
 of these species do, however, seem to retain their phenotypical
 characteristics despite the implication of gene flow, highlighting the
 remaining gap in our knowledge of how much of an organism's genome is
 permeable to gene flow, and which factors promote or prevent
 hybridization. We used AFLP markers to investigate the genetic composition
 of three populations involving two interfertile Rhododendron species: two
 sympatric populations, of which only one contained hybrids, and a further
 hybrid-dominated population. No fixed differences between the species were
 found, and only 5.8% of the markers showed some degree of species
 differentiation. Additionally, 45.5% of highly species-differentiating
 markers experienced significant transmission distortion in the hybrids,
 which was most pronounced in F1 hybrids, suggesting that factors conveying
 incompatibilities are still segregating within the species. Furthermore,
 the two hybrid populations showed stark contrasting composition of
 hybrids; one was an asymmetrically backcrossing hybrid swarm, while in the
 other, backcrosses were absent, thus preventing gene flow.
description: Original AFLP data
 matrixthis file has the
 following structure: - data for individuals are in rows, and loci are in
 columns - additionally, there are two columns in the beginning giving the
 population (pop), and species (species) of each individual; pop is either
 the population identifier [aga1 = aga-1, aga3 = aga-3, hyb1 = hyb-1, hyb2
 = hyb-2, pha1 = pha-1, pha3 = pha-3] or indicates individuals repeated for
 error estimation [con (control) = individuals repeated on each plate, with
 A-E as part of the identifier indicating the plate; test = individuals
 repeated on a different plate] - the last row (quality) gives an
 indication of the peak quality for each locus (separation from other
 peaks, clarity of peak) [A - very good, B - good, C - bad, X - extremely
 bad; intermediate categories, e.g. A-B for between A and B are used as
 well; generally A and B are good for use]ORIGINAL_AFLP_data.csvsimulated hybrids (R objects)7-zip archive containing the originally simulated hybrid populations; (sim.hyb.RData, sim.parent.RData) to use it copy the contents to /R/hybsimoriginal_data_R_hybsim.7zoriginal introgress results7-zip archive containing the original simulation results from introgress; (introgress.RData, sim.introgress.RData, introgress.test.l.locus.RData) to use it copy the contents to /R/introgressoriginal_data_R_introgress.7zoriginal Structure results7-zip archive containing the results from the structure runs for the original data; to use copy to /R/structure/resultsoriginal_results_structure.7zStructure results (simulations)7-zip archive containing the results from the structure runs for the simulated hybrids; to use copy to /R/structure/results_simoriginal_results_structure_sim.7zR and Structure scriptsScripts.7z