DOI: 10.15131/shef.data.7088906
title: Data for "Competitive species interactions constrain abiotic adaptation in a bacterial soil community"

description: This record contains .csv-formatted Supplementary Data tables associated with 'Competitive species interactions constrain abiotic adaptation in a bacterial soil community' by J. P. J. Hall et al. (2018). Sample analysis scripts for this data are provided in another ORDA record, doi:10.15131/shef.data.7043279.<br>SuppTab3.csv: .csv version of Supplementary Table 3. Fitness of evolved pQBR57-bearing <i>Pseudomonas fluorescens</i> SBW25 clones relative to their ancestor in soil microcosms. Six or seven clones were tested from populations evolved with or without mercury, and with or without <i>Pseudomonas putida</i> KT2440 competitors. Fitness was measured both with and without mercury. evo_rep = evolutionary replicate; cln_rep = clone reference; mrk = marker; cul = single-species (ss) or co-culture (co); evo_mer = mercury treatment in evolution experiment; test_mer = mercury in test conditions; fitness = relative fitness compared with ancestor (per day); fitness (generation) = relative fitness compared with ancestor (per generation).<br>SuppTab4.csv: .csv version of Supplementary Table 4. Mutations in <i>actP</i> detected in evolved <i>Pseudomonas fluorescens</i> SBW25 clones. sample_number = sample number in sequencing run; cln_name = code referring to that clone; evo_rep = evolutionary replicate; cln_rep = clone reference; pla = P if clone has pQBR57; evo_mer = mercury treatment in evolution experiment; pla_trt = plasmid treatment; mrk = marker; cul = cul = single-species (ss) or co-culture (co); mutation = <i>actP</i> mutation; effect = effect of mutation; impact = impact of mutation (defined by SNPeff); mutation_position = bp affected by mutation; effect_position = codon affected by position<br>SuppTab5.csv: .csv version of Supplementary Table 5. Growth of sequenced evolved <i>Pseudomonas fluorescens</i> SBW25 clones in soil microcosms with and without <i>Pseudomonas putida</i> KT2440 competitors. sample_number = sample number in sequencing run; cln_name = code referring to that clone; evo_rep = evolutionary replicate; cln_rep = clone reference; evo_mer = mercury treatment in evolution experiment; pla_trt = plasmid treatment; mrk = marker; cul = cul = single-species (ss) or co-culture (co); mutation = <i>actP</i> mutation; effect = effect of mutation; impact = impact of mutation (defined by SNPeff); mutation_position = bp affected by mutation; effect_position = codon affected by position; tech_rep = replicate experiment in which this clone was tested; m.with_putida = growth rate in co-culture (per day); m.alone = growth rate in single-species culture (per day); w = m.with_putida/m.alone<br>SuppTab6.csv: .csv version of Supplementary Table 6. Population density at transfer of <i>Pseudomonas fluorescens </i>SBW25 from the evolution experiment. rep = evolutionary replicate; tra = transfer; spc = species; cul = single-species (ss) or co-culture (co); pla_trt = plasmid treatment; mer = mercury treatment; mrk = marker; tot = cfu/g at transfer<br>SuppTab7.csv: .csv version of Supplementary Table 7. Relative growth of evolved <i>Pseudomonas fluorescens</i> SBW25 clones in acetate minimal media relative to glycerol minimal media. sample_number = sample number in sequencing run; cln_name = code referring to that clone; actp.status = disrupted or wild-type; actp.mutation = impact of the mutation on <i>actP</i>; technical.rep = replicate experiment in which this clone was tested; ratio = m_acetate/m_glycerol; difference = m_acetate - m_glycerol.<br>SuppTab8.csv: .csv version of Supplementary Table 8. Zones of inhibition produced by tellurite-impregnated discs for evolved <i>Pseudomonas fluorescens</i> SBW25 clones. sample_number = sample number in sequencing run; cln_name = code referring to that clone; tech_rep = replicate experiment in which this clone was tested; evo_mer = mercury treatment in evolution experiment; plasmid_trt = plasmid treatment; cul = single-species (ss) or co-culture (co); mutation = <i>actP</i> mutation; effect = effect of mutation; impact = impact of mutation (defined by SNPeff); actp_status = disrupted or wild-type; area = area of zone of inhibition (mm^2)<br>SuppTab9.csv: .csv version of Supplementary Table 9. Growth of evolved <i>Pseudomonas fluorescens</i> SBW25 clones in various concentrations of tellurite. sample_number = sample number in sequencing run; cln_name = code referring to that clone; tech_rep = replicate experiment in which this clone was tested; tell_conc = tellurite concentration (µg/ml); OD600_scaled = OD600 scaled between 0 and 1