DOI: 10.5061/dryad.ngf1vhj39
title: Data from: Micronuclear collapse from oxidative damage

description: Chromosome-containing micronuclei are a hallmark of aggressive cancers.
 Micronuclei frequently undergo irreversible collapse exposing their
 enclosed chromatin to the cytosol. Micronuclear rupture catalyzes
 chromosomal rearrangements, epigenetic abnormalities, and inflammation,
 yet mechanisms governing micronuclear integrity are poorly understood.
 Here we show that mitochondria-derived reactive oxygen species (ROS)
 disrupt micronuclei by promoting a noncanonical function of the ESCRT-III
 nuclear membrane repair complex protein, CHMP7. ROS retain CHMP7 in
 micronuclei while disrupting its interaction with other ESCRT-III
 components. Instead, ROS-induced cysteine oxidation promotes CHMP7
 oligomerization and binding to the nuclear membrane protein LEMD2
 disrupting micronuclear envelopes. We show that this ROS-CHMP7 axis
 promotes chromosome shattering known to result from micronuclear rupture.
 It also mediates micronuclear rupture under hypoxic conditions, linking
 tumor hypoxia with downstream processes driving cancer progression.
description: Flow cytometry files (.fcs) represent the distribution of cells
 in the cell cycle (DAPI signal) after double thymidine blockade or CDK1
 inhibition or a combination of both. Excel tables
 represent the source data for each figure and supplementary figure of the
 article-refer to online Materials and Methods for more information on how
 they have been collected. They are data underlying graphs and original
 immunoblot membranes. Every piece of data has a small caption near to it,
 to help identification.
description: # Data from:Micronuclear collapse from oxidative damage
 [https://doi.org/10.5061/dryad.ngf1vhj39](https://doi.org/10.5061/dryad.ngf1vhj39) ## Description of the data and file structure Flow cytometry (.fcs) files obtained by flow cytometry analysis of DAPI stained HeLa cells collected after double thymidine block, with or without 1uM CDK1 inhibition, and after 1 and 10uM CDK1 inhibition. The following files pertain to figure S12 F of the paper: 0h; 4h; 8h; 12h; 16h; 20h; 24h are the distribution of cells collected at the indicated time points after double thymidine block, while Unstained 1, 2 and 3 and DAPI 1, 2 and 3 are their negative control. The following files pertain to figure S12 J and K of the paper: DMSO1, 2, 3; Ro1 1,2,3; Ro10 1,2,3 are the distribution of cells in the cell cycle (DAPI stained) collected after 24h incubation with DMSO or Ro at 1uM or 10uM. The files Unstained1Ro2 and Unstained 2Ro2 are the negative controls. The following files pertain to figure S12 L of the paper: DTBDMSO 0,4,8,12; DTBRO 0,4,8,12; are cells collected at 0, 4, 8 or 12h after double thymidine blockade and treated after the blockade with either DMSO or RO 1uM. UnsyncDMSO 0 and 12 and UnsyncRO 0 and 12 are cells collected at 0 and 12 hours without double thymidine blockade and treated with DMSO or RO 1uM. The negative controls are* *DTB_Unstained All of the excel files are source data for each of the figures of the manuscript. Refer to Materials and Methods for more information on collection procedures. They are the raw data underlying graphs and original immunoblot membranes. Near each data set is a small caption to help identifying the specific graph they refer to. Each excel sheet refer to a figure or supplementary figure, while each tab refers to the specific panel in that figure (i.e. A, B, C..).  Cells marked with "null" correspond to cells in which no data were collected; while cells marked with "NA" correspond to cells without data because the measurement did not start yet or was completed already-for example, live cell imaging measurements that are normalized to a specific time point.