cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20012671.v1
title: Thermodymnamic properties of crambe fruits

description: ABSTRACT. This study aimed to determine and evaluate the thermodynamic properties of crambe fruit at different equilibrium moisture contents. The dynamic-gravimetric method was used to collect experimental data. Crambe fruits with an initial moisture content of approximately 26% db (dry basis) were used. The thin-layer desorption of the product was conducted at different controlled temperatures (25, 30, 35, 40, and 45°C) and at water activity levels ranging from 0.10 to 0.89 until the product reached the equilibrium moisture content at the specified air condition. In conclusion, the thermodynamic properties of crambe fruit are affected by temperature and moisture content. The isokinetic theory is valid for the desorption process, which is controlled by enthalpy, and the Gibbs free energy is positive at all of the tested temperatures, which indicates that water desorption is not a spontaneous process.
DOI: 10.6084/m9.figshare.20012676
title: Identification of QTL underlying agronomic, morphological and physiological traits in barley under rainfed conditions using SNP markers

description: ABSTRACT. Barley (Hordeum vulgare L.) is considered a good genetic model for evaluating mechanisms of drought tolerance, and it is the most important threat to crop production worldwide. This study aimed to identify single-nucleotide polymorphisms (SNPs) associated with agronomic, morphological and physiological traits in a population of 137 recombinant chromosome substitution lines (RCSL) of barley, which were evaluated under rainfed conditions, in Cauquenes, southern Chile (35°58’ S, 72°17’ W). The annual precipitation was 299 mm during the growing season. Fifty-two significant QTLs were detected for the studied traits, which explained between 5% and 13.8% of the phenotypic variation. A genomic region on chromosome 1H (that comprises SNPs 2711-234 and 1923-265) accounted for 13.4% and 13.8%, respectively, of the grain yield variation. In addition, SNPs 8388-578 and 7639-122 on the chromosome 5H had a moderate effect, explaining 12.8% of the plant height variation. Moreover, some SNPs were associated with more than one trait, and clusters of QTLs for yield and related traits were also found. Finally, the QTLs identified in the present study are of particular interest for barley-breeding purposes under rainfed conditions.
DOI: 10.6084/m9.figshare.20012676.v1
title: Identification of QTL underlying agronomic, morphological and physiological traits in barley under rainfed conditions using SNP markers

description: ABSTRACT. Barley (Hordeum vulgare L.) is considered a good genetic model for evaluating mechanisms of drought tolerance, and it is the most important threat to crop production worldwide. This study aimed to identify single-nucleotide polymorphisms (SNPs) associated with agronomic, morphological and physiological traits in a population of 137 recombinant chromosome substitution lines (RCSL) of barley, which were evaluated under rainfed conditions, in Cauquenes, southern Chile (35°58’ S, 72°17’ W). The annual precipitation was 299 mm during the growing season. Fifty-two significant QTLs were detected for the studied traits, which explained between 5% and 13.8% of the phenotypic variation. A genomic region on chromosome 1H (that comprises SNPs 2711-234 and 1923-265) accounted for 13.4% and 13.8%, respectively, of the grain yield variation. In addition, SNPs 8388-578 and 7639-122 on the chromosome 5H had a moderate effect, explaining 12.8% of the plant height variation. Moreover, some SNPs were associated with more than one trait, and clusters of QTLs for yield and related traits were also found. Finally, the QTLs identified in the present study are of particular interest for barley-breeding purposes under rainfed conditions.
DOI: 10.6084/m9.figshare.20012677
title: Inheritance of resistance to Meloidogyne incognita race 3 in cotton accession TX 25

description: ABSTRACT. Cotton producers worldwide suffer with the losses caused by the presence of phytonematodes. The aim of the present study was to investigate the inheritance of resistance to Meloidogyne incognita race 3 in Gossypium hirsutum variety punctatum accession TX 25. Accessions of Gossypium sp. were obtained from the germplasm bank of Embrapa Cotton. Two experiments were performed in two consecutive years. In the first experiment, a susceptible parental line, FiberMax 966, a resistant parental line, TX 25, and their F1, F2 and backcross generations were tested. In the second experiment, parental lines FiberMax 966 and TX 25, their F2 generation, and genotypes M315 (resistant), LA887 and DeltaOpal (moderately resistant) were tested. In both experiments, plants were inoculated with 2000 eggs and J2 of M. incognita race 3. The gall index, egg mass index and reproduction factor were evaluated 120 days following inoculation. In the first experiment, plants from the F1 and backcross generations were susceptible. Plants from the F2 generation presented a 3:1 resistant-to-susceptible ratio in the two experiments, indicating oligogenic resistance.
DOI: 10.6084/m9.figshare.20012677.v1
title: Inheritance of resistance to Meloidogyne incognita race 3 in cotton accession TX 25

description: ABSTRACT. Cotton producers worldwide suffer with the losses caused by the presence of phytonematodes. The aim of the present study was to investigate the inheritance of resistance to Meloidogyne incognita race 3 in Gossypium hirsutum variety punctatum accession TX 25. Accessions of Gossypium sp. were obtained from the germplasm bank of Embrapa Cotton. Two experiments were performed in two consecutive years. In the first experiment, a susceptible parental line, FiberMax 966, a resistant parental line, TX 25, and their F1, F2 and backcross generations were tested. In the second experiment, parental lines FiberMax 966 and TX 25, their F2 generation, and genotypes M315 (resistant), LA887 and DeltaOpal (moderately resistant) were tested. In both experiments, plants were inoculated with 2000 eggs and J2 of M. incognita race 3. The gall index, egg mass index and reproduction factor were evaluated 120 days following inoculation. In the first experiment, plants from the F1 and backcross generations were susceptible. Plants from the F2 generation presented a 3:1 resistant-to-susceptible ratio in the two experiments, indicating oligogenic resistance.
DOI: 10.6084/m9.figshare.20012679
title: Use of wild yeasts as a biocontrol agent against toxigenic fungi and OTA production

description: ABSTRACT. This study evaluated the antagonistic potential of 32 wild yeast isolates from coffee and cocoa bean fermentation. These yeasts were inoculated in co-cultivation with Aspergillus carbonarius (CCDCA 10608 and CCDCA 10408) and Aspergillus ochraceus (CCDCA 10612) isolated from grapes and coffee beans. The mycelial growth and ochratoxin A (OTA) production were evaluated, and the spores were counted after cultivation at 28°C for seven days. The yeasts presented higher inhibitory effects (53% in relation to the control) over the mycelial growth of the isolated A. ochraceus (CCDCA10612). Pichia anomala CCMA0148 and Saccharomyces cerevisiae CCMA0159 provided the greatest inhibition of the growth of all fungal strains. All Pichia species presented the highest inhibitory effects on the production of spores, and S. cerevisiae CCMA 0159 at concentrations of both 104 and 107 mL-1 cells inhibited the production of spores by 100%. Rhodotorula mucilaginosa was effective at inhibiting OTA production by the three isolates of Aspergillus. S. cerevisiae CCMA0159 and Pichia anomala CCMA0148 showed high potential as biocontrol agents in the conditions tested.
DOI: 10.6084/m9.figshare.20012679.v1
title: Use of wild yeasts as a biocontrol agent against toxigenic fungi and OTA production

description: ABSTRACT. This study evaluated the antagonistic potential of 32 wild yeast isolates from coffee and cocoa bean fermentation. These yeasts were inoculated in co-cultivation with Aspergillus carbonarius (CCDCA 10608 and CCDCA 10408) and Aspergillus ochraceus (CCDCA 10612) isolated from grapes and coffee beans. The mycelial growth and ochratoxin A (OTA) production were evaluated, and the spores were counted after cultivation at 28°C for seven days. The yeasts presented higher inhibitory effects (53% in relation to the control) over the mycelial growth of the isolated A. ochraceus (CCDCA10612). Pichia anomala CCMA0148 and Saccharomyces cerevisiae CCMA0159 provided the greatest inhibition of the growth of all fungal strains. All Pichia species presented the highest inhibitory effects on the production of spores, and S. cerevisiae CCMA 0159 at concentrations of both 104 and 107 mL-1 cells inhibited the production of spores by 100%. Rhodotorula mucilaginosa was effective at inhibiting OTA production by the three isolates of Aspergillus. S. cerevisiae CCMA0159 and Pichia anomala CCMA0148 showed high potential as biocontrol agents in the conditions tested.
DOI: 10.5519/qd.c4iigx8u
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (795 records)
DOI: 10.6084/m9.figshare.20012682.v1
title: Nitrogen management effects on soil mineral nitrogen, plant nutrition and yield of super early cycle common bean genotypes

description: ABSTRACT. This study was conducted to measure the effects of the timing of nitrogen fertilization (NF) on soil N-NH4 + and N-NO3 -, macronutrient contents in the leaves and grains, and the grain yield of super early genotypes (SEG) of common bean. Field experiments were performed for four growing seasons in an Oxisol soil in Santo Antônio de Goiás, Brazil, using a randomized block experimental design in a split plot scheme. The plots were planted with common bean SEG, and the subplots were the NF timings, including a treatment without N fertilization. The available soil N with improved fertility and medium to high levels of organic matter were enough to meet the N demands of the common bean crop in order to achieve yields of up to 3,000 kg ha-1. Fertilization with N, regardless of the time of application, provided no increase in the yield components, grain yield and the N, P and K contents in the leaves and grains of the common bean SEG. The genotypes CNFC 15873 and CNFC 15875 had higher grain yields in the rainfed summer, and the genotype CNFC 15874 had higher grain yields in the irrigated winter growing season. It can be inferred that the use of common bean SEG, with life cycles ranging from 65 to 77 days, can be a promising technology, providing grain yields similar to the control (IPR Colibri).
DOI: 10.6084/m9.figshare.20012682
title: Nitrogen management effects on soil mineral nitrogen, plant nutrition and yield of super early cycle common bean genotypes

description: ABSTRACT. This study was conducted to measure the effects of the timing of nitrogen fertilization (NF) on soil N-NH4 + and N-NO3 -, macronutrient contents in the leaves and grains, and the grain yield of super early genotypes (SEG) of common bean. Field experiments were performed for four growing seasons in an Oxisol soil in Santo Antônio de Goiás, Brazil, using a randomized block experimental design in a split plot scheme. The plots were planted with common bean SEG, and the subplots were the NF timings, including a treatment without N fertilization. The available soil N with improved fertility and medium to high levels of organic matter were enough to meet the N demands of the common bean crop in order to achieve yields of up to 3,000 kg ha-1. Fertilization with N, regardless of the time of application, provided no increase in the yield components, grain yield and the N, P and K contents in the leaves and grains of the common bean SEG. The genotypes CNFC 15873 and CNFC 15875 had higher grain yields in the rainfed summer, and the genotype CNFC 15874 had higher grain yields in the irrigated winter growing season. It can be inferred that the use of common bean SEG, with life cycles ranging from 65 to 77 days, can be a promising technology, providing grain yields similar to the control (IPR Colibri).
DOI: 10.6084/m9.figshare.20012683
title: Developing an in vitro optimized protocol to sweet potato landraces conservation

description: ABSTRACT. This study aimed to develop a protocol for the in vitro conservation of 30 accessions of sweet potato collected in the northern region of Rio de Janeiro State, Brazil, aiming to extend the period between subcultures for this species. An in vitro minimal growth experiment was established with accession UENF 1931 in a factorial arrangement consisting of four concentrations of mineral salts in MS medium and four concentrations of sucrose, at two temperatures. The others accessions were grown in medium containing 100% MS salts and 2% sucrose, which was the medium that allowed the in vitro conservation of accession UENF 1931 for a longer time period. For this second experiment, 30 accessions of sweet potato and two temperatures were used. Plant height, number of leaves, and survival rate were measured every 30 days for 12 months, in both experiments. In the last stage, the sweet potato plantlets originating from in vitro minimal growth were acclimatized and exhibited 100% survival. The recommended in vitro minimal growth conditions for the studied accessions are MS medium with 100% concentration of mineral salts and 2% sucrose, at a temperature of 27±2°C, with subculture performed every 180 days.
DOI: 10.6084/m9.figshare.20012683.v1
title: Developing an in vitro optimized protocol to sweet potato landraces conservation

description: ABSTRACT. This study aimed to develop a protocol for the in vitro conservation of 30 accessions of sweet potato collected in the northern region of Rio de Janeiro State, Brazil, aiming to extend the period between subcultures for this species. An in vitro minimal growth experiment was established with accession UENF 1931 in a factorial arrangement consisting of four concentrations of mineral salts in MS medium and four concentrations of sucrose, at two temperatures. The others accessions were grown in medium containing 100% MS salts and 2% sucrose, which was the medium that allowed the in vitro conservation of accession UENF 1931 for a longer time period. For this second experiment, 30 accessions of sweet potato and two temperatures were used. Plant height, number of leaves, and survival rate were measured every 30 days for 12 months, in both experiments. In the last stage, the sweet potato plantlets originating from in vitro minimal growth were acclimatized and exhibited 100% survival. The recommended in vitro minimal growth conditions for the studied accessions are MS medium with 100% concentration of mineral salts and 2% sucrose, at a temperature of 27±2°C, with subculture performed every 180 days.
DOI: 10.5519/qd.iuemq92n
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (779 records)
DOI: 10.6084/m9.figshare.5671285
title: Isolation and characterization of Bacillus thuringiensis strains active against Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae)

description: ABSTRACT. Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae) is an insect pest of 60 economically important crops, including sugarcane, wheat, soybean, rice, beans, sorghum, peanuts, and cotton. The aim of this work was to select and characterize Bacillus thuringiensis isolates with insecticidal activity against E. Lignosellus that could be used as an alternative method of control. Selective bioassays were done to evaluate the toxicity of 47 isolates against first instar larvae of E. lignosellus. For the most toxic bacterial strains, the lethal concentration (LC50) was estimated and morphological, biochemical and molecular methods were used to characterize the isolates. Among the 47 isolates tested, 12 caused mortality above 85% and showed LC50 values from 0.038E+8 to 0.855E+8 spores mL-1. Isolates BR83, BR145, BR09, BR78, S1534, and S1302 had the lowest LC50 values and did not differ from the standard HD-1 strain; the exception was BR83.The protein profiles produced bands with molecular masses of 60-130 kDa. The genes cry1, cry2, cry3, and cry11 were identified in the molecular characterization. The morphological analysis identified three different crystal inclusions: bipyramidal, spherical and cuboidal. Among the tested isolates, 12 isolates have potential for biotechnological control of E. Lignosellus by development of new biopesticides or genetically modified plants.
DOI: 10.6084/m9.figshare.5671285.v1
title: Isolation and characterization of Bacillus thuringiensis strains active against Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae)

description: ABSTRACT. Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae) is an insect pest of 60 economically important crops, including sugarcane, wheat, soybean, rice, beans, sorghum, peanuts, and cotton. The aim of this work was to select and characterize Bacillus thuringiensis isolates with insecticidal activity against E. Lignosellus that could be used as an alternative method of control. Selective bioassays were done to evaluate the toxicity of 47 isolates against first instar larvae of E. lignosellus. For the most toxic bacterial strains, the lethal concentration (LC50) was estimated and morphological, biochemical and molecular methods were used to characterize the isolates. Among the 47 isolates tested, 12 caused mortality above 85% and showed LC50 values from 0.038E+8 to 0.855E+8 spores mL-1. Isolates BR83, BR145, BR09, BR78, S1534, and S1302 had the lowest LC50 values and did not differ from the standard HD-1 strain; the exception was BR83.The protein profiles produced bands with molecular masses of 60-130 kDa. The genes cry1, cry2, cry3, and cry11 were identified in the molecular characterization. The morphological analysis identified three different crystal inclusions: bipyramidal, spherical and cuboidal. Among the tested isolates, 12 isolates have potential for biotechnological control of E. Lignosellus by development of new biopesticides or genetically modified plants.
DOI: 10.6084/m9.figshare.20012685
title: Stevia rebaudiana (Bert) Bertoni: influence of osmotic stress and seed priming on seed germination under laboratory conditions

description: ABSTRACT. The foremost factor necessary for plant growers cultivating large acreages of Stevia rebaudiana (Bert) Bertoni is the production of qualitative bedding plants. The objective of this study was to evaluate the influence of osmotic-priming on the uniformity of seed germination. First, we evaluated the percentage of normal seedlings from two seed samples harvested in 2011 and 2012. The seeds harvested in 2012 produced 71.4% normal seedlings and thus they were used in the next experiments. The seeds were subjected to osmotic stress using five concentrations of polyethylene glycol (PEG-6000) at -0.2, -0.4, -0.6, -0.8, and -1.0 MPa in contrast with distilled water. Based on these first results, only -0.8 and -1.0 MPa were evaluated in the third experiment. The seeds were immersed in both concentrations of polyethylene glycol (PEG-6000) for imbibing at 20ºC for four, five, six, and seven days. Thereafter, we evaluated the time to the first normal seedling (Ti), time to the last normal seedling (Tf), percentage normal seedlings at the initial time (Pi) and percentage of normal seedlings at the end of every treatment (Pf). Osmotic priming increased the percentage of normal seedlings of the Stevia rebaudiana and reduced the time to the first and last germination events.
DOI: 10.6084/m9.figshare.20012685.v1
title: Stevia rebaudiana (Bert) Bertoni: influence of osmotic stress and seed priming on seed germination under laboratory conditions

description: ABSTRACT. The foremost factor necessary for plant growers cultivating large acreages of Stevia rebaudiana (Bert) Bertoni is the production of qualitative bedding plants. The objective of this study was to evaluate the influence of osmotic-priming on the uniformity of seed germination. First, we evaluated the percentage of normal seedlings from two seed samples harvested in 2011 and 2012. The seeds harvested in 2012 produced 71.4% normal seedlings and thus they were used in the next experiments. The seeds were subjected to osmotic stress using five concentrations of polyethylene glycol (PEG-6000) at -0.2, -0.4, -0.6, -0.8, and -1.0 MPa in contrast with distilled water. Based on these first results, only -0.8 and -1.0 MPa were evaluated in the third experiment. The seeds were immersed in both concentrations of polyethylene glycol (PEG-6000) for imbibing at 20ºC for four, five, six, and seven days. Thereafter, we evaluated the time to the first normal seedling (Ti), time to the last normal seedling (Tf), percentage normal seedlings at the initial time (Pi) and percentage of normal seedlings at the end of every treatment (Pf). Osmotic priming increased the percentage of normal seedlings of the Stevia rebaudiana and reduced the time to the first and last germination events.
DOI: 10.6084/m9.figshare.5671084
title: Genetic admixture in species of Conyza (Asteraceae) as revealed by microsatellite markers

description: ABSTRACT. The distinction among Conyza canadensis, C. bonariensis, and C. sumatrensis is a challenge for weed science. In the current study, primers for microsatellite (SSR) loci were used to investigate the molecular divergence among the three species, the genetic structure of the populations at the molecular level and the level of genetic admixture among Conyza plants from southern Brazil. Twelve primers amplified well-defined DNA segments for all 88 samples of the three Conyza species. The estimated proportion of SSR polymorphic loci, number of alleles, and mean expected heterozygosity were higher in samples of C. bonariensis than in samples of C. sumatrensis or C. canadensis. Conyza canadensis was the species with the lowest molecular diversity. High genetic divergence was observed among the three species. The well-defined ancestral groups for each species led to the identification of samples of Conyza with ancestral genomes from the three species. Hybridization events between pairs of these species may have occurred in crop fields from southern Brazil. The high molecular diversity in resistant biotypes of C. sumatrensis indicated that these biotypes have a high potential to colonize new areas, which increases its potential as a weed.
DOI: 10.6084/m9.figshare.5671084.v1
title: Genetic admixture in species of Conyza (Asteraceae) as revealed by microsatellite markers

description: ABSTRACT. The distinction among Conyza canadensis, C. bonariensis, and C. sumatrensis is a challenge for weed science. In the current study, primers for microsatellite (SSR) loci were used to investigate the molecular divergence among the three species, the genetic structure of the populations at the molecular level and the level of genetic admixture among Conyza plants from southern Brazil. Twelve primers amplified well-defined DNA segments for all 88 samples of the three Conyza species. The estimated proportion of SSR polymorphic loci, number of alleles, and mean expected heterozygosity were higher in samples of C. bonariensis than in samples of C. sumatrensis or C. canadensis. Conyza canadensis was the species with the lowest molecular diversity. High genetic divergence was observed among the three species. The well-defined ancestral groups for each species led to the identification of samples of Conyza with ancestral genomes from the three species. Hybridization events between pairs of these species may have occurred in crop fields from southern Brazil. The high molecular diversity in resistant biotypes of C. sumatrensis indicated that these biotypes have a high potential to colonize new areas, which increases its potential as a weed.