DOI: 10.5281/zenodo.17136488
title: Microbiological and Chemical Characterisation of Sludges

description: Last updated: 2025-09-17DOI: 10.5281/zenodo.17136488

______Contact______ * Jana Rihova Ambrozova* jana.ambrozova@vscht.cz* +420 220 445 123* ORCID: 0000-0002-1503-409X* Dept. of Water Technology and Environmental Engineering, Faculty of Environmental Technology, University of chemistry and Technology, Prague* Technicka 5, 166 28, Prague 6, Czech Republic

______About the dataset______ In SBR reactors with activated sludge, laboratory testing of 12 types of PUR materials was carried out with the aim of determining the effect of PUR materials on the biocenosis of activated sludge. The reactors were operated so that 3 reactors were equipped with PUR materials and the fourth served as a control, in a total of 6 series PUR materials were tested: PUR_SA_DEG, PUR_SA_4EG, PUR_SA_TEG, PUR_A_2020, PUR_SA_UO.6, PUR_0,8_am, PUR_0.6, PUR_0.6-15S, PUR_0.8-15S, PUR_0.6-s05, PUR_0.6-s10, PUR_0.6-m05. A sample of activated sludge was taken from each reactor for chemical and microbiological analysis once a week. After the completion of each stage, PUR carriers were taken and leached. To determine the biochemical activity of microorganisms cultivated in individual reactors, kinetic tests of nitrification and denitrification were performed once a week. The development of biomass concentration in individual reactors was evaluated based on the determination of the concentration of suspended solids, also once a week. The pH value in the reactors was monitored every day. If necessary, the pH value was adjusted by dosing acid or alkali solutions. The influence of PUR materials on the biocenosis of activated sludge was evaluated by cultivation, selected groups of microorganisms were determined (thermotolerant coliform bacteria, E. coli, Cl. perfringens, P. aeruginosa, colony counts at 36 °C, nitrifying and denitrifying bacteria). The chemical indicators determined in the samples were inorganic forms of nitrogen (Namon, N-NO3-, N-NO2-), phosphorus (P-PO43-) and the concentration of organic substrate as a parameter CODCr.

Based on microbiological and chemical analyses, the PUR_0.8_am type carrier was installed in the real pilot plant of the Olovnice and Kladno WWTPs and monitored for a year.

While the data are in its esence numerical, the headers (and partially also file names) are in Czech language.

______Methods of data collection______ *For microbiological analyses (i.e. cultivation methods), sludge samples were diluted with physiological solution, selected groups of microorganisms were determined by cultivation (thermotolerant coliform bacteria, E. coli, Cl. perfringens, P. aeruginosa, colony counts at 36 °C, nitrifying and denitrifying bacteria). Microbiological analyses were performed in accordance with applicable national standards: thermotolerant coliform bacteria, E. coli (ČSN 75 7835), Cl. perfringens (Directive No. 252/2004 Coll., Appendix No. 6), P. aeruginosa (ČSN EN ISO 16266-2), colony counts at 36 °C (ČSN EN ISO 6222), nitrifying bacteria (Vinogradskij) nitrifying bacteria (Daubner). The number of KTJ or MPN in 1 ml of sludge sample was determined. The final result was determined by conversion to 1 g of dry matter.

Chemical analyses were performed in accordance with applicable national standards:* CHSK (ČSN ISO 15705)* Namon (ČSN ISO 7150-1)* N-NO3 (ČSN 75 7455)* N-NO2 (ČSN EN 26777)* P-PO4 (ČSN EN ISO 6875)* NL (ČSN EN 872)* The measurement was performed using a WTW PhotoLab 7100 spectrophotometer.* The results were expressed in mg/l for wastewater samples or in g/l for the determination of suspended solids in sludge suspension samples.

______Methods of data processing______ The dataset contains only raw data, therefore no processing is described.

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______File name structure_____ * ProjectName_AnalysesType_Descriptions.FileExtention* Example: NITRIPLAST_Microbiology_PilotPlant.csv

______File formats______* Tabular data – converted from XSLX to CSV(Files encoded in: Central European (Windows Latin 2))

______Date formats______* DD-MM-YYYY

______Units and abbreviations______ * KTJ/ml (CFU) – colony forming units per 1 mL* MPN/ml – most probable number per 1 mL* CLOP – Clostridium perfringens* DENITRIF – denitrifying bacteria* TCOLI – total coliforms* ECOLI – Escherichia coli* NITRIF – nitrifying bacteria* PSA – Pseudomonas aeruginosa* TB36 – culturable bacteria at 36 °C* TCOLI – thermotolerant bacteria* R1 – R4 – experimental reactor number* KT – kinetic test,* SBR – Sequencing Batch Reactor

* CHSK – chemical oxygen demand* NL – suspended solid* X – total biomass concentration* ZŽ – loss by burning* rV – process volume rate* rX, rX,o – specific process rates