DOI: 10.5281/zenodo.5728018
title: Dataset of ""Statistical atlases and automatic labelling strategies to accelerate the analysis of social insect brain evolution"

description: Dataset of <em>Statistical atlases and automatic labelling strategies to accelerate the analysis of social insect brain evolution</em> by Sara Arganda, Ignacio Arganda-Carreras, Darcy G. Gordon, Andrew P. Hoadley, Alfonso Pérez-Escudero, Martin Giurfa and James F. A. Traniello. In this dataset, we are presenting: 10 confocal brain images from <em>Pheidole spadonia </em>minors, with manually segmented labels of 8 subregions (Optic Lobes, OL; Antennal Lobes, AL; Mushroom Body Medial Calyx, MB-MC; Mushroom Body Lateral Calyx, MB-LC; Mushroom Body Peduncle, MB-P; Central Complex, CX; Subesophageal zone, SEZ; and Rest of Central Brain, ROCB) from one expert annotator. 12 confocal brain images from <em>P. spadonia</em>, <em>P. rhea</em>, <em>P. tepicana</em> and <em>P. obtusospinosa</em> minors, with manually segmented labels of the same 8 subregions (OL; AL; MB-MC; MB-LC; MB-P; CX; SEZ; and ROCB) from one expert annotator. 5 confocal brain images from <em>Pheidole spadonia </em>minors (“test brains”), with five sets of manually segmented labels of the same 8 subregions (OL; AL; MB-MC; MB-LC; MB-P; CX; SEZ; and ROCB) from three expert annotators (one set from annotator 1, one set from annotator 2 and three sets from annotator 3, to evaluate inter and intra person differences). 1 group-wise template generated from the 10 confocal brain images from <em>Pheidole spadonia </em>minors, with three sets of manually segmented labels of the same 8 subregions (OL; AL; MB-MC; MB-LC; MB-P; CX; SEZ; and ROCB). 5 group-wise templates generated from the 9 confocal brain images from <em>Pheidole spadonia </em>minors, with consensus labels of the same 8 subregions (OL; AL; MB-MC; MB-LC; MB-P; CX; SEZ; and ROCB). 1 group-wise template generated from 12 confocal brain images from <em>P. spadonia</em>, <em>P. rhea</em>, <em>P. tepicana</em> and <em>P. obtusospinosa</em> minors, with consensus labels of the same 8 subregions (OL; AL; MB-MC; MB-LC; MB-P; CX; SEZ; and ROCB). 7 sets of automatic labels for the 5 “test brains”: 3 sets of “Direct Labels”, 3 sets of “Consensus Labels”, 1 set of “Multispecies Template Labels”. Brain of minor workers were dissected from the ant head capsule in ice cold HEPES-buffered saline and were fixed and immunohistochemically stained using SYNORF1 (a monoclonal <em>Drosophila</em> synapsin I antibody obtained from the Developmental Studies Hybridoma Bank, catalog 3C11) and secondarily stained using Alexa Fluor 488 for visualization of neuropil (slightly modified from Ott, 2008). Later, brains were mounted in methyl salicylate and imaged on an Olympus Fluoview BX50 laser scanning confocal microscope with a ×20 objective at a resolution of ~0.7 × 0.7 × 5µm/voxel. All brain tissue manipulation, staining and recording was performed by Darcy G. Gordon. Manual segmentation of each brain was done using Amira (version 6.0 or 2019.2). Labels were traced on eight compartments in only one brain hemisphere, except for the CX, SEZ and ROCB, which lack a clear subdivision between hemispheres.