cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/e028s-29r33
title: CLEM of TRIM5a-YFP in HeLa cells

description: HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads 1/300. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS
description: <strong>Tilt Series Date:</strong> 2015-01-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 234.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-11/rawdata/</p>tomgraphytest20.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-11/rawdata/tomgraphytest20.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest20.rec,  Reconstruction (Pixel Size 2.4 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-11/3dimage_51229/tomgraphytest20.rec"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest20_full.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-11/3dimage_56135/tomgraphytest20_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kgg67-04g45
title: Adipocyte Cell

description: FGF21Dose-symmetrical dose scheme
description: <strong>Tilt Series Date:</strong> 2017-11-30</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Primary adipocyte cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 50000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Roger Kornberg and Maia</p>
<strong>Purification / Growth Conditions / Treatment:</strong> https://elifesciences.org/articles/43146#s4</p>
<strong>Sample Preparation:</strong> https://elifesciences.org/articles/43146#s4</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-27/rawdata/</p>tilt_series_20_sorted_orig.mrc,  Tilt Series (Pixel Size 2.241 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-27/rawdata/tilt_series_20_sorted_orig.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_20_sorted_orig_part61_15.rec,  Reconstruction (Pixel Size 8.964 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-27/3dimage_117269/tilt_series_20_sorted_orig_part61_15.rec"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_1_sorted_full.rec,  Reconstruction (Pixel Size 0.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-27/3dimage_118717/tilt_series_1_sorted_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hd82c-jd718
title: Hela-cryoFIB

description: Raw data files of Hela-cryoFIB
description: <strong>Tilt Series Date:</strong> 2017-03-27</p>
<strong>Data Taken By:</strong> Wei Zhao</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Hela cells grown on Au finder grids for 28-48 hours.Grids coated with PL.</p>
<strong>Sample Preparation:</strong> Sample Preparation:  Cells were plunge frozen using the vitrobot III blotted manually from back(drain time 1s).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-03-27-5/rawdata/</p>TS3.mrc,  Tilt Series (Pixel Size 0.372 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-03-27-5/rawdata/TS3.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS5_bin4_sirt8_flipint.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-03-27-5/3dimage_92897/TS5_bin4_sirt8_flipint.rec"
        > <i class="download icon"></i> Download </a></p>

        TS5_bin4_full.rec,  Reconstruction (Pixel Size 0.0 nm), 26.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-03-27-5/3dimage_92898/TS5_bin4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wh5ph-9r009
title: Adipocyte Cell

description: FGF21Dose-symmetrical dose scheme
description: <strong>Tilt Series Date:</strong> 2017-11-30</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Primary adipocyte cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 50000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Roger Kornberg and Maia</p>
<strong>Purification / Growth Conditions / Treatment:</strong> https://elifesciences.org/articles/43146#s4</p>
<strong>Sample Preparation:</strong> https://elifesciences.org/articles/43146#s4</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-26/rawdata/</p>tilt_series_16_sorted_orig.mrc,  Tilt Series (Pixel Size 2.241 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-26/rawdata/tilt_series_16_sorted_orig.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_16_sorted_orig_full.rec,  Reconstruction (Pixel Size 8.964 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-26/3dimage_117270/tilt_series_16_sorted_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_1_sorted_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-26/3dimage_118715/tilt_series_1_sorted_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/27h1t-abq57
title: #92+E.coli bamA purified

description: Raw data files of #92+E.coli bamA purified
description: <strong>Tilt Series Date:</strong> 2015-04-30</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chris Hayes and David Low UCSB</p>
<strong>Sample Preparation:</strong> These are E.cloacae mini cells (#92) mixed with purified E.coli BamA protein receptor. Both cells and BamA were blocked with BSA- 100g/ml for 30 minutes before mixing for 20 minutes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-30-13/rawdata/</p>PS96A-atlas0021.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-30-13/rawdata/PS96A-atlas0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        PS96A-atlas0021_full.rec,  Reconstruction (Pixel Size 1.32 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-30-13/3dimage_55357/PS96A-atlas0021_full.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2015-04-30-13_slicer19375.png, , 893.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2015-04-30-13_slicer19375.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.57830/2303487
title: Yellow-billed Cuckoo Inventory at Petrified Forest National Park 2023 - Open Format Dataset

description: Petrified Forest National Park is planning on removing invasive vegetation and restoring native plants along the Rio Puerco. Prior to removal, we conducted surveys for the federally Threatened Yellow-billed Cuckoo, following established survey protocols. Over the course of four surveys, we encountered two Yellow-billed cuckoos in each of two survey periods (four total detections, though possibly representing fewer than four birds). We did not detect any evidence of breeding behavior. We recommend continued monitoring for this species in the park, and proceeding with riparian restoration in accordance with methods that avoid negative impacts to Yellow-billed Cuckoos.
DOI: 10.57830/2304524
title: Fisheries Inventory, Invert Data, at Rocky Mountain National Park 2021-2022 - Open Format Dataset

description: The Cameron Peak Fire and East Troublesome Fire of 2020 were the two largest wildfires in Colorado history. They burned approximately 9% of the Rocky Mountain National Park, raising a concern for trout populations that currently support recreational fishing and success of on-going and future efforts to conserve native trout populations. In this study, we surveyed habitat characteristics and biological communities at 19 sites in summer of 2021 and a subset of 11 sites in summer of 2022 to characterize wildfire impacts on aquatic resources. We were primarily interested in trout population structure and abundance, which were compared to available pre-fire data. In addition, we collected information on physical habitat, water quality, algae and benthic macroinvertebrates because wildfire effects are complex and this array of data helps assist identifying ecological mechanisms that affect aquatic top predators (i.e., trout). The greenback cutthroat trout is listed as state and federally threatened and is the Colorado State Fish. The Colorado River cutthroat trout is a state species of concern and two distinct lineages occur in the burned areas of ROMO. Quantifying wildfire effects on stream ecosystems and trout populations is needed for ROMO to decide whether (1) ongoing conservation actions should continue including the Poudre Headwaters Project, (2) recreational fishing should be regulated, and (3) waters should require trout stocking or will naturally recover via trout immigration from connected populations. For more information see the report here: https://irma.nps.gov/DataStore/Reference/Profile/2304877
DOI: 10.22002/7zknm-3eb93
title: cryo-CLEM TDP-43

description: Osmotic shock (ask Qing Yao)Over expression of TDP-43-GFP
description: <strong>Tilt Series Date:</strong> 2019-06-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> U2OS cells</p>
<strong>Strain:</strong> TDP-43 expression</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Qing Yao</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on gold funder EM grids</p>
<strong>Sample Preparation:</strong> Manual Botting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-12/rawdata/</p>tomography5_orig.mrc,  Tilt Series (Pixel Size 2.678 nm), 5.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-12/rawdata/tomography5_orig.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography5_orig.rec,  Reconstruction (Pixel Size 10.712 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-12/3dimage_117272/tomography5_orig.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography5_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-12/3dimage_118739/tomography5_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qwq3q-68096
title: STIM1-YFP

description: hSTIM1-YFP stable STIM1 KO MEF
description: <strong>Tilt Series Date:</strong> 2020-10-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-58.0°, 58.0°), step: 2°, constant angular increment, dosage: 128.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter, Fang Yu, Khaled Machaca</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth on grid for 2 days, 5 ug Tg treated for 10min</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Gold finder, 10nm gold, 200 nm fluorescent bead, 95% . humidity, 37 deg C, twice manual blotting.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-13/rawdata/</p>tomo5.mrc,  Tilt Series (Pixel Size 0.265 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-13/rawdata/tomo5.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomo5_part59_4.rec,  Reconstruction (Pixel Size 1.06 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-13/3dimage_114918/tomo5_part59_4.rec"
        > <i class="download icon"></i> Download </a></p>

        tomo5_part59_5.rec,  Reconstruction (Pixel Size 1.06 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-28-13/3dimage_114968/tomo5_part59_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4r8tt-dx738
title: cryo-CLEM TDP-43

description: Osmotic shock (ask Qing Yao)Over expression of TDP-43-GFP
description: <strong>Tilt Series Date:</strong> 2019-06-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> U2OS cells</p>
<strong>Strain:</strong> TDP-43 expression</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Qing Yao</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on gold funder EM grids</p>
<strong>Sample Preparation:</strong> Manual Botting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-3/rawdata/</p>tomography6_orig.mrc,  Tilt Series (Pixel Size 2.678 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-3/rawdata/tomography6_orig.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography6_orig.rec,  Reconstruction (Pixel Size 10.712 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-3/3dimage_117242/tomography6_orig.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography6__bin8.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2019-06-19-3/3dimage_118740/tomography6__bin8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5wytn-4en65
title: wn19-2_sq1_cell2

description: WN19-2, sq1, IT 1s
description: <strong>Tilt Series Date:</strong> 2018-04-18</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 50.0°), step: 1°, constant angular increment, dosage: 168.0 eV/Å²,  defocus: -4.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> IDEM</p>
<strong>Sample Preparation:</strong> IDEM</p>
  wnj2018-04-18-2_slicer19450.jpg: Green arrows : stacked endomembranes and end of division density between the two cells. What is this? .   .  Blue and orange arrows : periplasmic densities
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-18-2/rawdata/</p>sq1_cell2.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-18-2/rawdata/sq1_cell2.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq1_cell2_full.rec,  Reconstruction (Pixel Size 1.2 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-18-2/3dimage_92453/sq1_cell2_full.rec"
        > <i class="download icon"></i> Download </a></p>

        sq1_cell2.rec,  Reconstruction (Pixel Size 2.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-18-2/3dimage_92489/sq1_cell2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qgbn5-rs024
title: Nitrosocaldus yellowstonensis

description: Raw data files of Nitrosocaldus yellowstonensis
description: <strong>Tilt Series Date:</strong> 2019-07-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Candidatus Nitrosocaldus yellowstonensis</p>
<strong>Strain:</strong> HL72</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jose R De La Torre and students, SF state, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R0.6/1 copper 300 mesh, 10nm gold, 22deg C, 100% humidity, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-7/3dimage_105631/</p>20190715_Ny_stationary_13_part59_5.rec,  Reconstruction (Pixel Size 1.0712 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-7/3dimage_105631/20190715_Ny_stationary_13_part59_5.rec"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_13.mrc,  Tilt Series (Pixel Size 0.2678 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-7/rawdata/20190715_Ny_stationary_13.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_13.mrc.mdoc,  31.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-7/rawdata/20190715_Ny_stationary_13.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>