DOI: 10.5061/dryad.gqnk98sv9
title: Data for: Fabrication and characterization of antibacterial coatings using an amphoteric condensed tannin,Tanfloc

description: This dataset presents comprehensive information on the development and
 characterization of antibacterial polyelectrolyte multilayer (PEM)
 coatings incorporating tanfloc, a plant-derived condensed tannin polymer
 with inherent antimicrobial properties. The dataset encompasses
 experimental data related to the fabrication of PEMs using tanfloc as
 either a polyanion or a polycation, expanding the versatility of this
 amphoteric polymer in PEM coatings. Typically, PEMs are formed by
 combining a polycation and a polyanion with complementary ionic groups.
 However, the unique amphoteric nature of tanfloc allows for the creation
 of PEMs utilizing only one of its functional groups, leaving the other
 functional group available for imparting antibacterial activity. The
 dataset includes details on the assembly of tanfloc-containing PEMs
 employing various counter-polyelectrolytes, including three poly-anionic
 glycosaminoglycans with varying charge densities, as well as the
 polycations N,N,N-trimethyl chitosan and polyethylenimine. The
 layer-by-layer assembly of PEMs is monitored using in situ
 Fourier-transform surface plasmon resonance (FT-SPR), confirming stable
 layer-by-layer construction. Surface chemistry is assessed through X-ray
 photoelectron spectroscopy (XPS). Furthermore, this dataset provides
 insights into the biocompatibility of tanfloc-containing PEMs,
 demonstrating their support for mammalian cells. Most notably, the dataset
 includes extensive data on the antiadhesive and antibacterial properties
 of these coatings against common implant-associated pathogens, such as
 Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial
 effects observed are attributed to electrostatic interactions and the
 polyphenolic nature of tanfloc.
description: <strong>1H Nuclear Magnetic Resonance (1H
 NMR):</strong> Tanfloc (TAN) was characterized using 1H NMR
 spectroscopy, recording spectra on a Bruker Neo US 400 NMR spectrometer
 operating at 400 MHz. <strong>Dynamic Light
 Scattering (DLS):</strong> Solutions of TAN (tanfloc) with varying
 pH values (5, 6, 7.4, 8.4, and 9.3) were prepared and subjected to dynamic
 light scattering (DLS) to determine the hydrodynamic diameter and
 polydispersity index (PDI). This characterization was performed using a
 Zetasizer Nano ZS instrument from Malvern.
 <strong>Fourier-Transform Surface Plasmon Resonance
 (FT-SPR):</strong> The layer-by-layer (LBL) assembly of PEMs on
 gold-coated glass chips was monitored in real-time using Fourier-Transform
 Surface Plasmon Resonance (FT-SPR). This involved modifying the
 gold-coated chips, flowing polycation, polyanion, and rinse solutions, and
 collecting data using an SPR-100 module coupled to a Nicolet 8700 FT-IR
 spectrometer. <strong>X-ray Photoelectron
 Spectroscopy (XPS): </strong>Composition analysis of the PEMs was
 performed using X-ray Photoelectron Spectroscopy (XPS). A survey scan was
 conducted over the energy range of 10 to 1100 eV, with high-resolution
 spectra obtained for the carbon (C1s), nitrogen (N1s), oxygen (O1s), and
 sulfur (S2p) envelopes. <strong>LDH Cytotoxicity
 Assay (ADSC cells):</strong> Human adipose-derived stem cells
 (ADSCs) at passage 6 were cultured in Dulbecco's Modified Eagle
 Medium (DMEM) supplemented with 10% fetal bovine serum and 1.0%
 penicillin/streptomycin. Sterilized samples were prepared and cells were
 seeded onto them. After 24 hours, culture media from each well were
 collected and subjected to the lactate dehydrogenase (LDH) cytotoxicity
 assay. This involved measuring absorbance at 490 nm and 680 nm using a
 plate reader (BMG LABTECH FLUOstar Omega). Control samples on polystyrene
 (PS) were included for comparison.
 <strong>Bacterial Growth Inhibition Assay:
 </strong>The antibacterial activity of different PEMs was assessed
 against both Gram-negative<em> Pseudomonas aeruginosa</em>
 (<em>P. aeruginosa</em>) and Gram-positive
 <em>Staphylococcus aureus (S. aureus)</em>. Bacteria were
 cultured in nutrient broth media (NBM) and diluted to a concentration of
 106 CFU/mL. Subsequently, 500 μL of the bacterial solution was exposed to
 the surfaces for 6 and 24 hours. After incubation, a 200 μL aliquot of the
 solution was extracted, and absorbance at 560 nm was measured using a
 plate reader. Control samples of glass and polystyrene (PS) were also
 included under identical conditions.
 <strong>Live-Dead Bacterial Cell
 Quantification:</strong> After incubation for 6 and 24 hours,
 bacterial adhesion to surfaces was visualized using fluorescence
 microscopy. The surfaces were stained and fixed before analysis. Live and
 dead bacterial percentages were determined using ImageJ software (n =
 3).
description: # Dataset for Fabrication and Characterization of Antibacterial Coatings
 using an Amphoteric Condensed Tannin, Tanfloc.
 The dataset contains
 experimental data detailing the development and characterization of
 antibacterial polyelectrolyte multilayer (PEM) coatings containing
 tanfloc, a natural condensed tannin polymer, as an amphoteric biomaterial.
 It includes results from biocompatibility assessments using human
 adipose-derived stem cells (ADSCs) and antibacterial activity assays
 against *Pseudomonas aeruginosa* and *Staphylococcus aureus*. Moreover,
 the dataset provides insights into tanfloc hydrodynamic properties, the
 real-time assembly of PEMs via Fourier-Transform Surface Plasmon Resonance
 (FT-SPR), and PEM compositional analysis by X-ray Photoelectron
 Spectroscopy (XPS). N,N,N-Trimethyl chitosan is also characterized via 1H
 Nuclear Magnetic Resonance (1H NMR) spectroscopy. Additionally,
 fluorescence microscopy data quantifies live and dead bacterial adhesion,
 demonstrating the antibacterial efficacy of the coatings. These diverse
 datasets are valuable for research in biomaterials and antimicrobial
 coatings. ## Description of the data and file structure We've
 organized the data into seven main folders, each containing valuable
 information related to various experiments and analyses. **Folder
 Structure** **1. H NMR** **Content:** This folder contains 1H Nuclear
 Magnetic Resonance (NMR) spectra, to confirm the synthesis of
 N,N,N-trimethyl chitosan. **File Format:** Spectra data is stored in .csv
 files. **File Name:** TMC H_NMR.csv **2. DLS** **Content:** In this
 folder, you'll find data related to the hydrodynamic diameter and
 polydispersity index of tanfloc solutions at different pH values (5, 6,
 7.4, 8.4, and 9.3) collected using Dynamic Light Scattering (DLS). **File
 Format:** Data is provided in .txt format. **File Name:** DLS_TAN.txt **3.
 FT_SPR** **Content:** Here, you'll discover real-time monitoring data
 for the deposition of Polyelectrolyte Multilayers (PEMs) using
 Fourier-Transform Surface Plasmon Resonance (FT-SPR). **File Format:**
 Data is stored in .csv files. **File Name:** FT_SPR.csv **4. XPS**
 **Content:** The X-ray Photoelectron Spectroscopy (XPS) folder is divided
 into two subfolders: **4.1. Survey:** **Content:** Contains XPS survey
 spectra related to various PEMs (TAN-HEP, TAN-HA, TAN-CS, TMC-TAN, and
 PEI-TAN), each assessed in 12 and 13 layers. **File Format:** Data is
 stored in .csv files. **File Names:** Survey.csv **4.2. High Resolution:**
 **Content:** Contains detailed high-resolution spectra for carbon (C1s),
 nitrogen (N1), oxygen (O1), and sulfur (S2p) envelopes of the same PEMs.
 **Subfolders:** There are ten subfolders corresponding to different PEMs,
 each with two layers (12 and 13). **TAN_HEP12** **File Format:** Data is
 stored in .csv files. **File Names:** C1.csv N1.csv O1.csv S2p.csv
 **TAN_HEP13** **File Format:** Data is stored in .csv files. **File
 Names:** C1.csv N1.csv O1.csv S2p.csv **TAN_HA12** **File Format:** Data
 is stored in .csv files. **File Names:** C1.csv N1.csv O1.csv **TAN_HA13**
 **File Format:** Data is stored in .csv files. **File Names:** C1.csv
 N1.csv O1.csv **TAN_CS12** **File Format:** Data is stored in .csv files.
 **File Names:** C1.csv N1.csv O1.csv S2p.csv **TAN_CS13** **File Format:**
 Data is stored in .csv files. **File Names:** C1.csv N1.csv O1.csv S2p.csv
 **TMC_TAN12** **File Format:** Data is stored in .csv files. **File
 Names:** C1.csv N1.csv O1.csv **TMC_TAN13** **File Format:** Data is
 stored in .csv files. **File Names:** C1.csv N1.csv O1.csv **PEI_TAN12**
 **File Format:** Data is stored in .csv files. **File Names:** C1.csv
 N1.csv O1.csv **PEI_TAN13** **File Format:** Data is stored in .csv files.
 **File Names:** C1.csv N1.csv O1.csv **5. LDH** **Content:** Lactate
 Dehydrogenase Assay (LDH) data related to absorbance at 490 nm and 680 nm,
 which indicates cytotoxicity. **File Format:** Data is provided in .csv
 format. **File Name:** LDH.csv **6. Bacterial Growth Inhibition**
 **Content:** This folder is further divided into two subfolders: **6.1.
 Staphylococcus aureus** **6.2. Pseudomonas aeruginosa** Each subfolder
 contains data files related to absorbance at 560 nm using a plate reader,
 indicating bacterial growth inhibition at two different time points (6 and
 24 hours). **File Format:** Data is stored in .csv files. **File Names:**
 bacteria_inhibition_6hr.csv bacteria_inhibition_24hr.csv **7. Live-Dead
 Bacterial Cell** **Content:** Similar to the Bacterial Growth Inhibition
 folder, this folder is also divided into two subfolders: **7.1.
 Staphylococcus aureus** **7.2. Pseudomonas aeruginosa** Each subfolder
 contains data files related to live and dead bacterial percentages
 determined using ImageJ software at two different time points (6 and 24
 hours). **File Format:** Data is stored in .csv files. **File Names:**
 6hr.csv 24hr.csv **Note:** 1\. FT_SPR Data Explanation: The
 'FT_SPR' data sheet contains numerical values reflecting
 measurements obtained from specific software during the designated period.
 These values vary across different samples due to variations in sample
 properties and measurement conditions. It is important to note that null
 values in this data sheet correspond to cells in the Excel file that were
 not reported by the software. These null values indicate a lack of
 reported data for those specific cells. 2\. Bacterial Growth Inhibition
 Data: The 'Bacterial Growth Inhibition' data is quantified using
 absorbance values, which are obtained through a plate reader. This
 instrument measures the absorbance of solutions placed in a 96-well plate.
 It is essential to understand that empty cells in this dataset should not
 be filled by users. These empty cells represent instances where
 measurements were either not applicable or not obtained, and thus should
 be left as is to maintain the integrity of the dataset. 3\. Live-Dead
 Bacterial Cell Data: For the 'Live-Dead Bacterial Cell' data, an
 effort was made to measure the number of cells in at least five different
 areas for each sample. Depending on the sample characteristics, this
 measurement could either be limited to exactly five points or include
 more. To maintain clarity and consistency in our dataset, empty cells,
 where measurements were not possible or not taken, have been filled with
 null values by the authors.