cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.8003136
title: [IO Islamic 383] لیلی و مجنون

description: <strong>Lailâ-u-Majnûn.</strong> <strong>This manuscript is now IO Islamic 1204 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 383 here with notations and hyperlinks].</strong> 1204 Lailâ u Majnûn. Another excellent copy of the <em>third</em> (in No. 1199 above the <em>second</em>) part of Amîr Khusrau’s Khamsah, dated by Sulṭân ‘Alî almashhadî the first of Ṣafar, A.H. 912 (A.D. 1506, June 23). The right order of ff. 89-94 is: 89, 91, 90, 93, 92, 94. No. 383, ff. 111, 2 coll., each ll. 12: very large and distinct Nasta’lîḳ; the first two pages splendidly illuminated; the margin throughout speckled with gold; size, 10¾ in. by 7 in.
DOI: 10.34740/kaggle/dsv/5842674
title: COVID Vaccination in World (updated daily)
DOI: 10.5281/zenodo.8003135
title: [IO Islamic 383] لیلی و مجنون

description: <strong>Lailâ-u-Majnûn.</strong> <strong>This manuscript is now IO Islamic 1204 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 383 here with notations and hyperlinks].</strong> 1204 Lailâ u Majnûn. Another excellent copy of the <em>third</em> (in No. 1199 above the <em>second</em>) part of Amîr Khusrau’s Khamsah, dated by Sulṭân ‘Alî almashhadî the first of Ṣafar, A.H. 912 (A.D. 1506, June 23). The right order of ff. 89-94 is: 89, 91, 90, 93, 92, 94. No. 383, ff. 111, 2 coll., each ll. 12: very large and distinct Nasta’lîḳ; the first two pages splendidly illuminated; the margin throughout speckled with gold; size, 10¾ in. by 7 in.
DOI: 10.5281/zenodo.8003144
title: [IO Islamic 736] هشت بهشت

description: <strong>Hasht Bihisht and Â’îna-i-Iskandarî.</strong> <strong>This manuscript is now IO Islamic 1205 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 736 here with notations and hyperlinks].</strong> 1205 Hasht Bihisht and Â’îna-i-Iskandarî. Another copy of the <em>fourth</em> and <em>fifth</em> part of Amîr Khusrau’s Khamsah, without a date. 1.Hasht Bihisht, on fol. 270<sup>b</sup>. 2.Â’îna-i-Iskandarî (here simply styled اسكندر نامه), on fol. 306<sup>b</sup>. All the chapter-headings are left blank. No. 736, ff. 270-353, 4 coll., each ll. 25; distinct Nasta’lîḳ; an illuminated frontispiece at the beginning of either mathnawî; size, 15<sup>1/8</sup> in. by 9<sup>7/8</sup> in.
DOI: 10.5281/zenodo.8003143
title: [IO Islamic 736] هشت بهشت

description: <strong>Hasht Bihisht and Â’îna-i-Iskandarî.</strong> <strong>This manuscript is now IO Islamic 1205 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 736 here with notations and hyperlinks].</strong> 1205 Hasht Bihisht and Â’îna-i-Iskandarî. Another copy of the <em>fourth</em> and <em>fifth</em> part of Amîr Khusrau’s Khamsah, without a date. 1.Hasht Bihisht, on fol. 270<sup>b</sup>. 2.Â’îna-i-Iskandarî (here simply styled اسكندر نامه), on fol. 306<sup>b</sup>. All the chapter-headings are left blank. No. 736, ff. 270-353, 4 coll., each ll. 25; distinct Nasta’lîḳ; an illuminated frontispiece at the beginning of either mathnawî; size, 15<sup>1/8</sup> in. by 9<sup>7/8</sup> in.
DOI: 10.6069/c0qa-zg66
title: Evaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data Independent Acquisition

description: We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data independent acquisition, the Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Orbitrap mass spectrometers, which have long been the gold standard for high resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer is capable of producing high quality quantitative measurements across a wide dynamic range. We also use a newly developed extra-cellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5,000 plasma proteins in a 60-minute gradient with the Orbitrap Astral mass spectrometer.
DOI: 10.22002/caw8h-mmq67
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-30/rawdata/</p>Bsubtilis_293_015_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-30/rawdata/Bsubtilis_293_015_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_015_af_part39_4.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-30/3dimage_111635/Bsubtilis_293_015_af_part39_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/6es-y8f-yfz
title: Plantae

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/g7m-n2v-dhc
title: Bovidae Gray, 1821

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.15468/dl.7xumeh
title: Occurrence Download

description: A dataset containing 414544 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry MULTIPOLYGON(((-74.34115 4.60362,-74.33492 4.59475,-74.33016 4.57565,-74.32007 4.55961,-74.29404 4.53671,-74.28034 4.52885,-74.26164 4.52387,-74.24585 4.52392,-74.20895 4.53092,-74.1984 4.53572,-74.18969 4.54213,-74.18117 4.55191,-74.17521 4.56345,-74.17215 4.57608,-74.17215 4.58888,-74.17528 4.6017,-74.18117 4.61305,-74.18983 4.62297,-74.20055 4.63045,-74.19048 4.63521,-74.16331 4.64368,-74.14758 4.65439,-74.13626 4.66862,-74.13026 4.68065,-74.11824 4.69311,-74.11164 4.70284,-74.1062 4.71843,-74.10559 4.73406,-74.06164 4.76064,-74.04761 4.77562,-74.03935 4.7956,-74.02152 4.80359,-74.00722 4.81756,-73.99802 4.83617,-73.9933 4.85156,-73.99187 4.86329,-73.99537 4.8832,-74.00562 4.90015,-74.01343 4.90735,-74.02249 4.91289,-74.03245 4.91655,-74.04412 4.91824,-74.0616 4.91596,-74.07747 4.90814,-74.09092 4.90401,-74.10827 4.89331,-74.11671 4.88613,-74.13296 4.86545,-74.14304 4.84284,-74.15467 4.8317,-74.16548 4.82691,-74.1754 4.81977,-74.19509 4.79767,-74.21215 4.80238,-74.22978 4.80226,-74.2512 4.79438,-74.2654 4.7848,-74.27658 4.77003,-74.29376 4.75881,-74.30496 4.74596,-74.31179 4.72961,-74.31448 4.70767,-74.32648 4.69658,-74.33238 4.695,-74.34433 4.68717,-74.35324 4.67599,-74.35822 4.66257,-74.35796 4.64267,-74.35303 4.62522,-74.34634 4.61085,-74.34115 4.60362)))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 414544 records from 303 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0012037-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/5ptc5-2jf78
title: Shewanella MR1_Iron cores_Chemos

description: Raw data files of Shewanella MR1_Iron cores_Chemos
description: <strong>Tilt Series Date:</strong> 2018-08-09</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These are Shewanella oneidensis MR1 prepared by Sahand (USC) by Chemostat method grown for 2 days and chemically fixed. I (Mohammed) imaged these cells on the Polara to see if the have the iron cores in order to send them to Israel (Sharon Wolf) to perform EDX and confirm the components of the iron cores.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Chemostat 2 days growth and chemically fixed. The samples were prepared by Sahand (USC).</p>
<strong>Sample Preparation:</strong> I prepared these samples by blot force 6, blot time 3s and drain time 1. I also used the Bdellovibrio conditions (force -5, time 4 s). 10 nm Gold nanoparticles were added.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-70/rawdata/</p>SheweTilt34.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-70/rawdata/SheweTilt34.mrc"
        > <i class="download icon"></i> Download </a></p>

        SheweTilt34_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-70/3dimage_94163/SheweTilt34_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jey84-xae66
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-118/rawdata/</p>Hpylori_DFliF_FISE_118.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-118/rawdata/Hpylori_DFliF_FISE_118.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_118_part41_13.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-118/3dimage_113716/Hpylori_DFliF_FISE_118_part41_13.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/ls8-v88-xls
title: Pteropoda Cuvier, 1804

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/644tn-es578
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-5/rawdata/</p>Bsubtilis_293_044_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-5/rawdata/Bsubtilis_293_044_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_044_af_part41_11.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-5/3dimage_111392/Bsubtilis_293_044_af_part41_11.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/3lg-err-6jv
title: Lithothamnion Heydrich, 1897

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qzr-mzr-w86
title: Bourgueticrinina Sieverts-Doreck, 1953

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/a8p3f-fa626
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-92/rawdata/</p>Bsubtilis_293_093_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-92/rawdata/Bsubtilis_293_093_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_093_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-92/3dimage_111551/Bsubtilis_293_093_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/vsy-qdk-q97
title: Echinoidea Schumacher, 1817

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/a33f5-1dw13
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-180/rawdata/</p>Hpylori_DFliF_FISE_183.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-180/rawdata/Hpylori_DFliF_FISE_183.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_183_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-180/3dimage_113843/Hpylori_DFliF_FISE_183_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/096x2-g8h74
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-196/rawdata/</p>Hpylori_DFliF_FISE_200.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-196/rawdata/Hpylori_DFliF_FISE_200.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_200_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-196/3dimage_113864/Hpylori_DFliF_FISE_200_full.rec"
        > <i class="download icon"></i> Download </a></p>