DOI: 10.5061/dryad.73n5tb383
title: Warm waters undermine cryptic female choice

description: (1)  Understanding the thermal sensitivity of reproductive
 interactions is crucial given global warming. Previous studies have almost
 exclusively focused on interactions before mating, even though important
 interactions between the sexes also occur after mating (e.g., gamete
 interactions), which are likely affected by temperature. (2) 
 Thus, it remains unknown how temperature affects the influence of female
 reproductive fluid on sperm performance, thereby altering female control
 over fertilization (cryptic female choice). This gap limits our
 understanding of how sexual selection changes with seasonal temperature
 fluctuations and temperatures outside the range of historical norms.
 (3)  We tested how temperatures relevant to current conditions
 and climate change projections influence the mechanisms underlying cryptic
 female choice in a marine fish, Symphodus ocellatus. Under typical thermal
 conditions, female reproductive fluid enhances sperm velocity and biases
 fertilization dynamics to favor preferred, dominant males over sneaker
 males. (4)  We find that warmer temperatures decrease female
 influence on sperm velocity, especially for dominant males. This results
 in dominant males having slower sperm than sneaker males at warmer
 temperatures, reducing the expected paternity of preferred, dominant
 males. (5)  Our results highlight that considering the thermal
 sensitivity of female-male interactions that occur after mating will be
 essential for understanding how seasonal variation and climate change can
 influence fertility, reproduction, and sexual selection.
description: Description of code and data files affiliated with "*Warm waters
 undermine cryptic female choice*" by Matthew C. Kustra, Louise M.
 Alissa, Michaela M. Rogers, Megan M. Molinari, Kelly A. Stiver, Susan
 Marsh-Rollo, Jennifer K. Hellmann, and Suzanne H. Alonzo For questions,
 contact: matthewckustra@gmail.com To properly run the code, make sure all
 data files are in the same directory as the code
 *"Figures_Analysis.R*"* Below is a description of all the code
 and data files broken down by the directories in which code and data files
 should be organized in. All code for figures and analysis are in a single
 file: **Figures_Analysis.R** uploaded to **Zenodo**. There is also the
 **"SessionInfo.txt"** file that provides information about
 hardware and package versions code was run with. Here is a summary of the
 different Data files used by **Figures_Analysis.R** *
 "**Temp_CFC_Data.csv**": Data file that contains experimental
 data used in the paper. Here are the descriptions of the column variables:
 * ***Sperm.number***: Individual ID of the sperm being tracked. *
 ***VCL***: Curvillinear sperm velocity (μm/s) * ***Second_view***: Time
 since activation (s) that the sperm was recorded. * ***Field***: The field
 number or short video clip ID that the sperm was measured in. All sperm in
 the same field of the same file have the same second_view. *
 ***filename***: Name of the file. * ***frames***: How many frames an
 individual sperm was tracked for * ***Male***: Male ID * ***Female***:
 Female ID * ***Trial***: Trial ID * ***Temp***: Test temperature of the
 experiment (16,22,28; Celsius). * ***OF***: Activating fluid used in the
 experiment. Either "OF" (diluted ovarian fluid) or
 "SW" (seawater). * ***MaleT***: Male reproductive type. Either
 "NM" for nesting male or "SN" for sneaker. *
 "**Alonzo_et_al_Nature_Comm_2016_data.csv"**": Data file
 from Alonzo et. al 2016
 [https://doi.org/10.1038/ncomms12452](https://doi.org/10.1038/ncomms12452). This data file is a reduced version of "Alonzo_et_al_Nature_Comm_2016_Expt1.csv" on Dryad ([https://doi.org/10.5061/dryad.1m056](https://doi.org/10.5061/dryad.1m056)) * ***NM_sperm_number***: Average number of nesting male sperm. * ***SN_sperm_number***: Average number of sneaker male sperm. * ***NM1.VCL***: Nesting male sperm velocity at 40 seconds. * ***SN1.VCL***: Sneaker male sperm velocity at 40 seconds. * ***Number_Assigned_NM***: The number of offspring assigned to the nesting male in the genetic paternity analyses. * ***Number_Genotyped***: The number of offspring genotyped. * ***Ovarian_Fluid_Treatment***: Ovarian fluid treatment. Either "Baseline" for ovarian fluid or "Rinsed" for no ovarian fluid. * "**SST_1981_2023_08-09_raw.rds**": Data file of longterm temperature data collected from STARESO presented in Figure 1A,b. Data was provided by Lovina Fullgrabe (Fullgrabe et al. 2020. [https://doi.org/10.1016/j.marenvres.2020.104962](https://doi.org/10.1016/j.marenvres.2020.104962)) . Long-term acquisition of temperature data was supported by the STARESO marine station, the Rhone-Mediterranean and Corsica Water Agency and the Collectivity of Corsica under the STARECAPMED project. **Data set is not posted on this depository**.  The column names and descriptions are below: * ***tssta_codesta*** Device that recorded the temperature measurment. * ***daytime***: Date and time. Date is in the formate of year-month-day * ***Temperature***: Temperature recorded (C) * ***Pression abs. kPA***: Absolute pressure in kPA. * "**DateEX.csv**": Data file that contains information about the date that experiments were conducted.The column names and descriptions are below: * ***Trial***: Experimental ID to match with "**Temp_CFC_Data.csv**" * ***Date***: Date of the experiment in Year-Month-Day format. * "**STARESO_2.5mDeep_HOBO_Corse23.csv**": Data file of sea tempearture data collected by the authors of this paper during the course of the experiment near nests of the ocellated wrasse. The column names and descriptions are below: * ***year***: Year of temperature data collection. * ***day***: Date of temperature data collection in month/day/year format. * ***time***: Time of data collection in Hour:Minute:Second * ***temp***: Temperature recorded (C)