cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25402/bio.23266760.v1
title: Effect of chronopharmacology and food on in vivo pharmacokinetic profile of mavacamten: supplementary materials

description: Aim: This study investigated the impact of food intake and circadian rhythms on the pharmacokinetics of
mavacamten. Materials & methods: A sensitive bioanalytical method for quantifying mavacamten in rat
plasma was developed and validated. Thismethod was applied to assess the effect of chronopharmacology
and food intake on the pharmacokinetics of mavacamten in rats. Results: A circadian variation at two
doses resulted in significant changes in the volume of distribution, clearance and time ofmaximum plasma
concentration of mavacamten (p &lt; 0.05). In addition, food intake had an insignificant impact on the
pharmacokinetic parameters except for the time of maximum plasma concentration (p&lt;0.05). Conclusion:
These pharmacokinetic changes and human chronotype findings will help optimize dosing time.
DOI: 10.25549/examiner-c44-75500
title: Spring School Dance Festival (Cowan Avenue School), 1954

description: 12 images. Spring School Dance Festival (Cowan Avenue School), 21 May 1954. Laurel Goldsmith - 6 years; Kenny Blake - 6 years; Tommy Stephens; John O'Neill; Jim Thompson; June Hadsell; Jimmy McKenzie; Marvia Brown; Jeffrey Olsen.; Caption slip reads: "Photographer: Olmo. Date: 1954-05-21. Reporter: Petty. Assignment: School Dance Festival. 63-64: First graders in a 'Children's Polka'. 65-66: Second graders in 'Little Brown Jug' folk dance. 67-68: Fifth graders in square dance. 70: Third graders in 'La Raspa' Mexican dance. 69: Tommy Stephens, John O'Neill & Jim Thompson accompanying dance. 71-72: Long shots of crowd. 73: (R) Laurel Goldsmith and (L) Kenny Blake, both 6 years old, first graders, in polka. 74: June Hadsell and Jimmy McKenzie (foreground) and Marvia Brown and Jeffrey Olsen, back couple.".
DOI: 10.25549/examiner-c44-74582
title: Kiwanis Rose Bowl Kick-Off Luncheon in Pasadena, 1956

description: 4 images. Kiwanis Rose Bowl Kick-Off Luncheon in Pasadena, 31 December 1956. Reed Culp; Tommy Prothro (coach); Forest Evashevski (coach).; Caption slip reads: "Photographer: Jensen. Date: 1956-12-31. Reporter: Kiwanis Rose Bowl Kick-off Luncheon, Pasadena. L/R Reed Culp, Pres. Kiwanis International; Coach Tommy Prothro; Coach Forest Evashevski. L/R Coaches alone; Tommy Prothro; Forest Evashevski.
DOI: 10.25402/bio.23500713
title: Volumetric absorptive microsampling as an effective microsampling technique for LC–MS/MS bioanalysis of biomarkers in drug discovery: Supplementary data

description: Supplementary tables
DOI: 10.25402/bio.23500713.v1
title: Volumetric absorptive microsampling as an effective microsampling technique for LC–MS/MS bioanalysis of biomarkers in drug discovery: Supplementary data

description: Supplementary tables
DOI: 10.25402/bio.23574444
title: An efficient LC–MS/MS method for simultaneous determination of four substances in the Hcy metabolism pathway: supplementary materials

description: Aim: This study aimed to develop and validate an efficient LC–MS/MS method for the simultaneous
determination of Hcy, Cys, Met and 5-methyltetrahydrofolate in human serum and to apply this method
to patients with coronary artery disease. Methodology and results: Serum samples were prepared by
reduction with dithiothreitol followed by protein precipitation. The analytical run time was 2.2 min.
The linearity was good in the range of 2–100 μmol/l-1 for Hcy and Met, 10–500 μmol/l-1 for Cys, and
1–50 ng/ml-1 for 5-methyltetrahydrofolate. Conclusion: An accurate and precise method that was rapid,
robust and with high-throughput for the routine clinical monitoring of patients with coronary artery
disease was developed.
DOI: 10.25402/bio.23574444.v1
title: An efficient LC–MS/MS method for simultaneous determination of four substances in the Hcy metabolism pathway: supplementary materials

description: Aim: This study aimed to develop and validate an efficient LC–MS/MS method for the simultaneous
determination of Hcy, Cys, Met and 5-methyltetrahydrofolate in human serum and to apply this method
to patients with coronary artery disease. Methodology and results: Serum samples were prepared by
reduction with dithiothreitol followed by protein precipitation. The analytical run time was 2.2 min.
The linearity was good in the range of 2–100 μmol/l-1 for Hcy and Met, 10–500 μmol/l-1 for Cys, and
1–50 ng/ml-1 for 5-methyltetrahydrofolate. Conclusion: An accurate and precise method that was rapid,
robust and with high-throughput for the routine clinical monitoring of patients with coronary artery
disease was developed.
DOI: 10.25549/examiner-c44-19914
title: United States Naval Base, boxing, bowling, basketball, 1948

description: 14 images. United States Naval Base, boxing, bowling, basketball, 17 November 1948. Linwood Bowser; Perry Eastep; Robert Tuthill; Richard Freeman; George Jones; Coach Jake Shugrue; Jack Nelson; Billie Hodger; Lieutenant John Popov.
DOI: 10.25402/bio.23574450
title: Preclinical pharmacokinetics of 4-hydroxy isoleucine using LC–MS/MS: a potential polycystic ovary syndrome phytopharmaceutical therapeutics: supplementary tables

description: Aim: To study the preclinical pharmacokinetics of 4-hydroxy isoleucine (4-HIL) targeted for polycystic
ovary syndrome. Methodology: The quantitative bioanalysis of 4-HIL in different biological matrices
in female Sprage-Dawley rats using LC–MS/MS. Results: At 50 mg/kg, 4-HIL had 56.8% absolute
oral bioavailability. It was quickly absorbed and distributed in various tissues in order of small
intestine &gt; kidney &gt; ovary &gt; spleen &gt; lung &gt; liver &gt; heart &gt; brain after oral administration. Moreover,
11.07% of 4-HIL was recovered in urine and feces within 72 h. Conclusion: 4-HIL levels in vital organs were
found safe, as per tissue distribution results. Hence, 4-HIL could be used as promising therapeutics for
management of polycystic ovary syndrome.
DOI: 10.25402/bio.23574450.v1
title: Preclinical pharmacokinetics of 4-hydroxy isoleucine using LC–MS/MS: a potential polycystic ovary syndrome phytopharmaceutical therapeutics: supplementary tables

description: Aim: To study the preclinical pharmacokinetics of 4-hydroxy isoleucine (4-HIL) targeted for polycystic
ovary syndrome. Methodology: The quantitative bioanalysis of 4-HIL in different biological matrices
in female Sprage-Dawley rats using LC–MS/MS. Results: At 50 mg/kg, 4-HIL had 56.8% absolute
oral bioavailability. It was quickly absorbed and distributed in various tissues in order of small
intestine &gt; kidney &gt; ovary &gt; spleen &gt; lung &gt; liver &gt; heart &gt; brain after oral administration. Moreover,
11.07% of 4-HIL was recovered in urine and feces within 72 h. Conclusion: 4-HIL levels in vital organs were
found safe, as per tissue distribution results. Hence, 4-HIL could be used as promising therapeutics for
management of polycystic ovary syndrome.
DOI: 10.25549/examiner-c44-15090
title: Trombino-Brancato murder suspect (Hollywood), 1951

description: 2 images. Trombino-Brancato murder suspect (Hollywood), 25 August 1951. Joe Flever (suspect); Detective E.M. Goosen; Detective G.G. Greeley.
DOI: 10.25402/bio.23660082
title: Gas chromatography properties and mass spectrometry fragmentation of anabolic androgenic steroids in doping control: Supplementary data

description: Aim: This study aimed to investigate the gas chromatographic properties and mass spectrometric
fragmentations of anabolic androgenic steroids (AASs) after trimethylsilylated derivatization. Materials
& methods: A total of 113 AASs were analyzed through gas chromatography-mass spectrometry in the
full-scan mode. Results: New fragmentation pathways yielding m/z 129, 143 and 169 ions were analyzed.
Based on the characteristics of the A-ring, seven classes of drugs were identified and analyzed. Conclusion:
The fragmentation pathway of a new classification of 4-en-3-hydroxyl was reported for the first time. The
relationship between the chemical structures of AASs and their retention time, along with their molecular
ion peak abundance, was also reported herein for the first time.
DOI: 10.25402/bio.23660082.v1
title: Gas chromatography properties and mass spectrometry fragmentation of anabolic androgenic steroids in doping control: Supplementary data

description: Aim: This study aimed to investigate the gas chromatographic properties and mass spectrometric
fragmentations of anabolic androgenic steroids (AASs) after trimethylsilylated derivatization. Materials
& methods: A total of 113 AASs were analyzed through gas chromatography-mass spectrometry in the
full-scan mode. Results: New fragmentation pathways yielding m/z 129, 143 and 169 ions were analyzed.
Based on the characteristics of the A-ring, seven classes of drugs were identified and analyzed. Conclusion:
The fragmentation pathway of a new classification of 4-en-3-hydroxyl was reported for the first time. The
relationship between the chemical structures of AASs and their retention time, along with their molecular
ion peak abundance, was also reported herein for the first time.
DOI: 10.25549/examiner-c44-18855
title: San Fernando fair races, 1951

description: 4 images. San Fernando fair races, 31 August 1951.; Caption slip reads: "Photographer: Jensen. Date: 08-31. Assignment: San Fernando fair races. H-9: First race into first turn: #1-2-3-4. H-10: Second race, first turn: 1-2-3-4. J96: Third race. J-97: First quarter horse race".; Supplementary material reads: "Official program, quarter horse races, Thursday, August 30, 1951, Devonshire Downs, Northridge, California...First, Second and Third Races with the horse names, number and color; the jockey riding each horse and the owners are listed. (For a complete list, please see the original)".
DOI: 10.25402/bio.23660088
title: A smartphone digital image colorimetric method based on nanoparticles for determination of lamotrigine: Supplementary material

description: Aim: A colorimetric approach for quantification of lamotrigine using spectrophotometric and smartphone
image analysis is described in this study. Methods: For full optimization and validation procedures, UVvisible
spectroscopy was used, and image analysis was carried out with the help of an app (PhotoMetrix
PRO R ? ). Then, as a multivariate calibration method, parallel factor analysis was used for data analysis.
Results: The results demonstrated the capacity of these methods to estimate lamotrigine concentrations
in the range of 0.1–7.0 μg.ml-1 in exhaled breath condensate, indicating the value of using digital images
and smartphone applications in combination with chemometric tools. Conclusion: The image analysis can
be superior for its fast and reliable lamotrigine analysis in biological samples.
DOI: 10.25402/bio.23660088.v1
title: A smartphone digital image colorimetric method based on nanoparticles for determination of lamotrigine: Supplementary material

description: Aim: A colorimetric approach for quantification of lamotrigine using spectrophotometric and smartphone
image analysis is described in this study. Methods: For full optimization and validation procedures, UVvisible
spectroscopy was used, and image analysis was carried out with the help of an app (PhotoMetrix
PRO R ? ). Then, as a multivariate calibration method, parallel factor analysis was used for data analysis.
Results: The results demonstrated the capacity of these methods to estimate lamotrigine concentrations
in the range of 0.1–7.0 μg.ml-1 in exhaled breath condensate, indicating the value of using digital images
and smartphone applications in combination with chemometric tools. Conclusion: The image analysis can
be superior for its fast and reliable lamotrigine analysis in biological samples.
DOI: 10.25549/examiner-c44-20021
title: Chemical Society commemorative stamp, 1951

description: 5 images. Chemical Society Commemorative Stamp, 5 September 1951. Stamp (copy); Sheldon F. Wirth (clerk); Donna Martel (Movie Actress); Michael D. Fanning (Postmaster).; Supplementary material reads: Stewart. 1951-05-09. Memo to City Desk: Larry Anderson, Ma-7411, Ex-258, says there will be a photo set up in the Postmaster Fanning's office today at 11 a.m. Fanning will sell the first American Chemical Society Commemorative Stamp to Mongram movie actress Donna Martel. Officials of the Chemical Society will be on hand. 9:50 a.m.".; Additional supplementary material reads: "September 5. Photo Room: In pictures coming in on Chemical Society commemoration stamp, in background, is a clerk whose name not included in story. If want to identify him, he is Sheldon F. Wirth. Gus Newman".
DOI: 10.25402/bio.23967264
title: A sensitive LC–MS/MS assay to quantitatefree payload Aur0101 from ADC PYX-201 inrat and monkey plasma: supplementary materials

description: Aim: Aur0101 is a cytotoxic and small-molecule microtubule depolymerizing agent, and is the payload conjugated to antibody–drug conjugate PYX-201. Developing and validating a sensitive bioanalytical method to quantitate Aur0101 was novel and crucial in preclinical PYX-201 studies. Materials & methods: Reference standard Aur0101 and its stable isotope labelled internal standard Aur0101-d8 were used in this LC–MS/MS method. Results: This sensitive assay was validated at a lower limit of quantitation of 15 pg/ml and successfully applied to support preclinical rat and monkey toxicology studies. Preclinical plasma toxicokinetic parameters were presented. Conclusion: A sensitive and robust LC–MS/MS assay was validated for Aur0101 in rat and monkey plasma.
DOI: 10.25402/bio.23967264.v1
title: A sensitive LC–MS/MS assay to quantitatefree payload Aur0101 from ADC PYX-201 inrat and monkey plasma: supplementary materials

description: Aim: Aur0101 is a cytotoxic and small-molecule microtubule depolymerizing agent, and is the payload conjugated to antibody–drug conjugate PYX-201. Developing and validating a sensitive bioanalytical method to quantitate Aur0101 was novel and crucial in preclinical PYX-201 studies. Materials & methods: Reference standard Aur0101 and its stable isotope labelled internal standard Aur0101-d8 were used in this LC–MS/MS method. Results: This sensitive assay was validated at a lower limit of quantitation of 15 pg/ml and successfully applied to support preclinical rat and monkey toxicology studies. Preclinical plasma toxicokinetic parameters were presented. Conclusion: A sensitive and robust LC–MS/MS assay was validated for Aur0101 in rat and monkey plasma.
DOI: 10.25549/examiner-c44-9354
title: Premature twins, 1951

description: 4 images. Premature twins, May 6, 1951. Mrs. Leona Shannon -- 16 years (mother); Maurice Shannon -- 19 years; Barbara Shannon; Diane and Sue Shannon -- 1 month old.
DOI: 10.25402/bio.24042366
title: Evaluating the use of locked nucleicacid capture probes in hybrid LC–MS/MSanalysis of siRNA analytes: supplementary materials

description: Background: Hybrid LC–MS assays for oligonucleotides rely on capture probes to develop assays with high sensitivity and specificity. Locked nucleic acid (LNA) probes are thermodynamically superior to existing capture probes, but are not currently used for hybrid LC–MS assays. Materials & methods: Using two lipid-conjugated double-stranded siRNA compounds as model analytes, hybrid LC–MS/MS assays using LNA probes were developed. Results: The workflows demonstrated the superiority of the LNA probes, optimized sample preparation conditions to maximize analyte recovery, evaluated the need for analytespecific internal standards, and demonstrated that advanced mass spectrometric technology can increase assay sensitivity by up to 20-fold. Conclusion: The workflow can be used in future bioanalytical studies to develop effective hybrid LC–MS/MS methods for siRNA analytes.
DOI: 10.25549/examiner-c44-10774
title: Questionable death, 1951

description: 4 images. Questionable death, 24 July 1951. David Lauren -- 22 years (victim, copy); Rita Lauren -- 21 years (informed of husband's death).
DOI: 10.25402/bio.24042366.v1
title: Evaluating the use of locked nucleicacid capture probes in hybrid LC–MS/MSanalysis of siRNA analytes: supplementary materials

description: Background: Hybrid LC–MS assays for oligonucleotides rely on capture probes to develop assays with high sensitivity and specificity. Locked nucleic acid (LNA) probes are thermodynamically superior to existing capture probes, but are not currently used for hybrid LC–MS assays. Materials & methods: Using two lipid-conjugated double-stranded siRNA compounds as model analytes, hybrid LC–MS/MS assays using LNA probes were developed. Results: The workflows demonstrated the superiority of the LNA probes, optimized sample preparation conditions to maximize analyte recovery, evaluated the need for analytespecific internal standards, and demonstrated that advanced mass spectrometric technology can increase assay sensitivity by up to 20-fold. Conclusion: The workflow can be used in future bioanalytical studies to develop effective hybrid LC–MS/MS methods for siRNA analytes.
DOI: 10.25549/examiner-c44-74610
title: Town hall, 1957

description: 2 images. Town hall, 15 January 1957. J. Kenneth Galbraith; Horace N. Gilbert (President of Town Hall).; Caption slip reads: "Photographer: Rustan. Date: 1957-01-15. Assignment: Town hall. 51/52: L to R - J. Kenneth Galbraith & Horace N. Gilbert, Pres of Town Hall. 35/36: J. Kenneth Galbraith".
DOI: 10.25402/bio.24085350
title: A blood-based, metabolite and demographiccharacteristic markers panel for thediagnosis of Alzheimer’s disease: supplementary material

description: Aims: This work was designed to provide early diagnosis strategies for Alzheimer’s disease (AD) based onthe identification of blood metabolic biomarkers. Patients & methods: A total of 90 subjects aged 60 yearsor older were included in this study; 45 patients were assigned to the case group and control group,respectively. A total of 31 targetmetabolites were quantitatively analyzed by parallel reaction monitoringbetween the two groups. Results & conclusion: Three metabolites were screened out, including cystine,serine and alanine/sarcosine. Logistic regression and random forest analysis were used to establish ADdiagnosis models, and the model combining metabolic biomarkers and demographic variables had higherdetection efficiency (area under the curve = 0.869). A combination diagnostic model to provide a scientificreference for early screening and diagnosis of AD was constructed.
DOI: 10.25402/bio.24085350.v1
title: A blood-based, metabolite and demographiccharacteristic markers panel for thediagnosis of Alzheimer’s disease: supplementary material

description: Aims: This work was designed to provide early diagnosis strategies for Alzheimer’s disease (AD) based onthe identification of blood metabolic biomarkers. Patients & methods: A total of 90 subjects aged 60 yearsor older were included in this study; 45 patients were assigned to the case group and control group,respectively. A total of 31 targetmetabolites were quantitatively analyzed by parallel reaction monitoringbetween the two groups. Results & conclusion: Three metabolites were screened out, including cystine,serine and alanine/sarcosine. Logistic regression and random forest analysis were used to establish ADdiagnosis models, and the model combining metabolic biomarkers and demographic variables had higherdetection efficiency (area under the curve = 0.869). A combination diagnostic model to provide a scientificreference for early screening and diagnosis of AD was constructed.
DOI: 10.25549/examiner-c44-20390
title: Society...Saint Vincent's Hospital Auxiliary Party, 1951

description: 2 images. Society...Saint Vincent's Hospital Auxiliary Party, 10 September 1951. Countess Estelle Doheny; Mrs. John B. Rauen; Mrs. W.R. Molony, Junior; Mrs. Harold B. Meloth; Miss Ann O'Brien. (Sleeve reads: S9302).; Caption slip reads: "Photographer: Merc[urio]. Assignment: Society. 12-109: Mrs. Harold B. Meloth; Miss Ann O'Brien; Countess Estelle Doheny; Mrs. John B. Rauen; and Mrs. W.R. Molony, Jr.".
DOI: 10.25549/examiner-c44-74520
title: Baby found in auto, 1956

description: 6 images. Baby found in auto, 27 February 1956. Doctor Nick Famularo; Thomas Benjamin Bernard (20 months); Mrs. Bernice Bernard.; Caption slip reads: "Photographer: Olmo. Date: 1956-02-27. Reporter: Brown. Assignment: Baby found in auto. Dr. Nick Famularo holds Thomas Benjamin Bernard, 20 months, at Hollywood Receiving while awaiting Mom. Mrs. Bernice Bernard, 23, reunited with son, Thomas Benjamin, 20 months".
DOI: 10.25402/bio.24106062
title: Volumetric absorptive microsamplingcoupled with hybridization LC–MS/MS forquantitation of antisense oligonucleotides: supplementary materials

description: Background: Volumetric absorptive microsampling has emerged as a less invasive alternative to venoussampling for small-molecule pharmacokinetic studies, but its application to novel therapeutics such asantisense oligonucleotides (ASOs) is not well-established. Results: A workflow was developed usingMitra microsampling coupled with hybridization LC–MS/MS for accurate determination of fomivirsen,a 21-mer ASO, in human blood. Quantitative recovery was achieved regardless of blood hematocritlevel or microsample age by implementing impact-assisted extraction. A thorough method evaluationconfirmed sensitivity, linearity, precision/accuracy,matrix effect, metabolite interference and four monthsof microsample stability. Conclusion: The combined impact-assisted extraction and hybridization LC–MS/MS workflow demonstrated the successful quantitation of fomivirsen, establishing the validity andapplicability of the approach for ASO drug candidates.
DOI: 10.25402/bio.24106062.v1
title: Volumetric absorptive microsamplingcoupled with hybridization LC–MS/MS forquantitation of antisense oligonucleotides: supplementary materials

description: Background: Volumetric absorptive microsampling has emerged as a less invasive alternative to venoussampling for small-molecule pharmacokinetic studies, but its application to novel therapeutics such asantisense oligonucleotides (ASOs) is not well-established. Results: A workflow was developed usingMitra microsampling coupled with hybridization LC–MS/MS for accurate determination of fomivirsen,a 21-mer ASO, in human blood. Quantitative recovery was achieved regardless of blood hematocritlevel or microsample age by implementing impact-assisted extraction. A thorough method evaluationconfirmed sensitivity, linearity, precision/accuracy,matrix effect, metabolite interference and four monthsof microsample stability. Conclusion: The combined impact-assisted extraction and hybridization LC–MS/MS workflow demonstrated the successful quantitation of fomivirsen, establishing the validity andapplicability of the approach for ASO drug candidates.
DOI: 10.25549/examiner-c44-20141
title: Contempt of court, 1951

description: 2 images. Contempt of court, 6 September 1951. Helen O'Connell (Smith) -- 31 years; Clifford Smith, Junior.; Caption slip reads: "Photographer: Gaze. Date: 1951-09-06. Reporter: Lumen. Assignment: Helen O'Connell. #33: Helen O'Connell, 31, singer, and husband, Clifford Smith, Jr., leave conference room following Santa Monica Superior Court session in which he was held in contempt of court for failure to keep up temporary maintenance and child support payments pending hearing on her suit for separate maintenance. #34: Helen O'Connell Smith".