cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ppdec-74d85
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2012-11-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 115.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-28/rawdata/</p>Bdellovibrio_wt_20121114_0010013.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-28/rawdata/Bdellovibrio_wt_20121114_0010013.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdellovibrio_wt_20121114_0010013_full.rec,  Reconstruction (Pixel Size 1.6 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-28/3dimage_23534/Bdellovibrio_wt_20121114_0010013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qvs4k-wde58
title: UU 2801- P221D delta P1P2

description: Strain P221D (low kinase activity) with P1P2 deletion in CheA
description: <strong>Tilt Series Date:</strong> 2012-09-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase, then lysed for one hour with Penicillin</p>
<strong>Sample Preparation:</strong> cells plunge frozen on Quantifoul R2/2 in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-9/rawdata/</p>Coli2801atlas0917_10005.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-9/rawdata/Coli2801atlas0917_10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        Coli2801atlas0917_10005_part130_1.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-9/3dimage_20824/Coli2801atlas0917_10005_part130_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8y5ces
title: Occurrence Download

description: A dataset containing 23262 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Potentilla indica",
 "Country is Belgium",
 "HasCoordinate is true"
 ]
}

 The dataset includes 23262 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045816-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/5dz0r-ba820
title: CheA-Ge8-L2

description: This strain lacks a chromosomal CheA, and carries plasmid pj207 containing a CheA with a trimer of the silver peptide in linker region  two.
description: <strong>Tilt Series Date:</strong> 2012-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Dan Feldheim and Carly Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB plus Kanamycin, induced with 14nM IPTG for one hour, penicillin treated for an additional hour</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-7/rawdata/</p>CheAmutantmanual3.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-7/rawdata/CheAmutantmanual3.mrc"
        > <i class="download icon"></i> Download </a></p>

        CheAmutantmanual3_part128_7.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-7/3dimage_17933/CheAmutantmanual3_part128_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s6dfx-sq392
title: Agro VirB8-GFP

description: Raw data files of Agro VirB8-GFP
description: <strong>Tilt Series Date:</strong> 2013-10-03</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown in liquid at 19C and induced with 200uM AS for 24 hours</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-12/rawdata/</p>virb8_24h_2750013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-12/rawdata/virb8_24h_2750013.mrc"
        > <i class="download icon"></i> Download </a></p>

        virb8_24h_2750013_full.rec,  Reconstruction (Pixel Size 1.56 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-12/3dimage_31836/virb8_24h_2750013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0xzz3-mgt20
title: HeLa cells

description: Looking for ESCRT around midbodies
description: <strong>Tilt Series Date:</strong> 2014-11-26</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> Cells provided by Doug Mckarty</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA nup153+Thymidine 2mM for 14 h.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-14/rawdata/</p>midbody30004.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-14/rawdata/midbody30004.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody30004_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-14/3dimage_48363/midbody30004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yydmx-jns21
title: Crosslinked HIV-1 cores TRIM5a

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5alpha.
description: <strong>Tilt Series Date:</strong> 2013-05-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor and SIRT reconstruction</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-9/3dimage_42961/</p>atlas0009_part121_62.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-9/3dimage_42961/atlas0009_part121_62.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas0009.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-9/rawdata/atlas0009.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g8j71-y9839
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2012-11-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 82.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-37/rawdata/</p>Bdellovibrio_wt_20121114_0030002.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-37/rawdata/Bdellovibrio_wt_20121114_0030002.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdellovibrio_wt_20121114_0030002_full.rec,  Reconstruction (Pixel Size 1.32 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-37/3dimage_23550/Bdellovibrio_wt_20121114_0030002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b9gb1-7cq77
title: A. longum ProteinaseK digest

description: A. longum's cell wall was digested with proteinase K to determine the periplasmic layer composition
description: <strong>Tilt Series Date:</strong> 2012-04-26</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Wesley Chen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown anaerobically and digested with 5mg/ml proteinase K enzyme</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-26-2/rawdata/</p>alongprotk0002.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-26-2/rawdata/alongprotk0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        alongprotk0002_full.rec,  Reconstruction (Pixel Size 3.8 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-26-2/3dimage_18240/alongprotk0002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p02j1-wh897
title: Agrobacterium C58 25C plate liq

description: C58 wild type
description: <strong>Tilt Series Date:</strong> 2014-05-30</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown at 25 on plates for 2 days then recovered in liquid for 5 h</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
  etl2014-05-30-13_slicer6234.jpg: filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-30-13/3dimage_43074/</p>c58_25_pl_liq0006_part121_10.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-30-13/3dimage_43074/c58_25_pl_liq0006_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>

        c58_25_pl_liq0006.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-30-13/rawdata/c58_25_pl_liq0006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6c05v-j4n23
title: Agrobacterium tumefaciens

description: Bald strain, flagellin A, B and C missing. D is present
description: <strong>Tilt Series Date:</strong> 2013-10-30</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on AB medium and induced with AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-6/rawdata/</p>bald20004.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-6/rawdata/bald20004.mrc"
        > <i class="download icon"></i> Download </a></p>

        bald20004_full.rec,  Reconstruction (Pixel Size 1.6 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-6/3dimage_33664/bald20004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/khae0-gj350
title: Vibrio delta-CheA1

description: Vibrio cholerae strain deleted for CheA1
description: <strong>Tilt Series Date:</strong> 2014-03-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Ikuro Kawagishi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-04-18/rawdata/</p>deltaA1VibrioC0018.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-04-18/rawdata/deltaA1VibrioC0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        deltaA1VibrioC0018_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-04-18/3dimage_39655/deltaA1VibrioC0018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6m77d-2qc52
title: Crosslinked HIV-1 cores TRIM5a

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5alpha.
description: <strong>Tilt Series Date:</strong> 2013-05-24</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 99.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-24-4/rawdata/</p>montage0004.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-24-4/rawdata/montage0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage0004.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 711.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-24-4/3dimage_47841/montage0004.sirt.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fgnr7-4kf56
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-05-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-22/3dimage_69241/</p>12may04_C126_3_pnlDN_Cos7_AT4BT1_0004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
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        12may04_C126_3_pnlDN_Cos7_AT4BT1_0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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