cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0571c27
title: CIL:10109, Rattus, multipolar neuron. In Cell Image Library

description: This multi-layer image shows the spatial relationship between filamentous actin (red) and microtubule array (green) in cultured hippocampal neurons, grown for 3 days in vitro. Actin staining (with rhodamine phalloidin) highlights the growing tips and filopodial extensions along axons and dendrites, while microtubule staining reveals the stable shafts of these processes. Some nonneuronal cells may also appear in the field.
Detailed Methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, 37°C, 15 minutes), permeabilized (0.25% Triton, 7 minutes) and immunostained for tubulin (monoclonal DM1A, Sigma, with Alexa 488 conjugated secondary, Molecular Probes, excitation, 494, emission, 519) and rhodamine-conjugated phalloidin (Molecular Probes, excitation, 540, emission, 565). Fluorescent and phase images were acquired with a Leica DMRA microscope with a mercury arc lamp, a 20X lens (HC PL Fluotar, NA 0.5), Leica GFP filter set (excitation, BP 470/40; dichromatic mirror, 500, suppression filter, BP 525/50); Leica N3 filter set (excitation, BP546/12; dichromatic mirror, 565, suppression filter, BP 600/40), Photometrics CoolSnap ES CCD camera and MetaMorph software.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
DOI: 10.6075/j0445kkm
title: CIL:10103, Homo sapiens, epithelial cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody; Fluorescein (FITC)
Processing history: unprocessed raw data
description: Cultured retinal pigment epithelial cells immunofluorescently labeled for adaptor protein-2 (AP2) (green) and nucleus (blue). The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA. AP2 was detected with AP-6 primary antibody and secondary FITC antibody. The nucleus was detected with DAPI staining. Images were collected on an Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for AP2 and 50ms exposure for DAPI (67nm/pixel).
DOI: 10.6075/j0c24vmg
title: CIL:10102, Homo sapiens, epithelial cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody; Fluorescein (FITC)
Processing history: unprocessed raw data
description: Cultured retinal pigment epithelial cells immunofluorescently labeled for clathrin (green) and nucleus (blue). The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA. Clathrin was detected with X22 primary antibody and secondary FITC antibody. The nucleus was detected with DAPI staining. Images were collected on an Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for clathrin and 50ms exposure for DAPI (67nm/pixel).
DOI: 10.6075/j0vx0fnd
title: CIL:10060, Mus musculus, tracheal epithelial cell. In Cell Image Library

description: Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed using ice-cold methanol for 10 min. Samples were stained using antibodies against acetylated tubulin to mark cilia (green), the centriole protein Cep120 to mark basal bodies (red) and cell-cell junctions using ZO-1 (blue). Secondary antibodies were Alexa 488 for acetylated tubulin, Alexa 594 for Cep120, and Cy5 for ZO-1. Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) and a 100X 1.3 NA objective.
description: Preparation: methanol fixed tissue
Relation to intact cell: detergent permeabilized
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific nucleic acid sequence
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; Alexa Fluor 594; Cy5
Processing history: unprocessed raw data
DOI: 10.6075/j0zc8217
title: CIL:10055, Rana catesbeiana, barrier epithelial cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Processing history: unprocessed raw data
description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system. The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation. The coils appear to stack in loose hexagonal arrays (CIL:9301). The plasma membrane of these cells has many caveolae, as illustrated in this micrograph, which also shows several large intermediate filament bundles and desmosomes - insertion sites at the plasma membrane for the intermediate filaments. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6075/j0ks6qq1
title: CIL:10054, Rana catesbeiana, barrier epithelial cell. In Cell Image Library

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system. The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation, and some coils are closely associated with the nuclear envelope, as is shown in this micrograph. The coils appear to stack in loose hexagonal arrays (see CIL:9301). The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0s75ff3
title: CIL:10051, Rana catesbeiana, barrier epithelial cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Processing history: unprocessed raw data
description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6075/j04q7t83
title: CIL:1011, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 1 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
DOI: 10.6075/j0r210md
title: CIL:1010, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 1 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j0p84bwq
title: CIL:10097, Rattus, multipolar neuron. In Cell Image Library

description: This multi-layer image shows the spatial relationship between filamentous actin (red) and microtubule array (green) in cultured hippocampal neurons, grown for 1 day in vitro. Actin staining (with rhodamine phalloidin) highlights the growing tips and filopodial extensions along axons and dendrites, while microtubule staining reveals the stable shafts of these processes. Some nonneuronal cells may also appear in the field.
Detailed Methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, 37°C, 15 minutes), permeabilized (0.25% Triton, 7 minutes) and immunostained for tubulin (monoclonal DM1A, Sigma, with Alexa 488 conjugated secondary, Molecular Probes, excitation, 494, emission, 519) and rhodamine-conjugated phalloidin (Molecular Probes, excitation, 540, emission, 565). Fluorescent and phase images were acquired with a Leica DMRA microscope with a mercury arc lamp, a 20X lens (HC PL Fluotar, NA 0.5), Leica GFP filter set (excitation, BP 470/40; dichromatic mirror, 500, suppression filter, BP 525/50); Leica N3 filter set (excitation, BP546/12; dichromatic mirror, 565, suppression filter, BP 600/40), Photometrics CoolSnap ES CCD camera and MetaMorph software.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
DOI: 10.6075/j0251hbv
title: CIL:10101, Homo sapiens, epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: mCherryFP; EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell time lapse images were collected on a Nikon TiE TIRF with a 100x1.49 NA objective (~67nm/pixel) using 120 ms exposures. This single dual color image part of an image series that contains the time lapse for the mCherry-clathrin light chain and the EGFP-epsin movies as separate files.
DOI: 10.15468/dl.j55bnf
title: Occurrence Download

description: A dataset containing 625 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is The vascular plants collection (P) at the Herbarium of the Muséum national d'Histoire Naturelle (MNHN - Paris)",
 "TaxonKey is Afzelia"
 ]
}

 The dataset includes 625 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009635-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0jh3mjb
title: CIL:57234, Ebola virus - Mayinga, Zaire, 1976, Huh7. In Cell Image Library

description: Pixel size: 83.92 Å; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
description: Ebola virus Huh7 infected cells 14 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
DOI: 10.6075/j01j9b4m
title: CIL:57263. In Cell Image Library

description: Ebola virus Huh7 infected cells 18 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Pixel size: 8.25 Å ; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
DOI: 10.6075/j0p84c82
title: CIL:57232, Ebola virus - Mayinga, Zaire, 1976, Huh7. In Cell Image Library

description: Ebola virus Huh7 infected cells 14 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
description: Pixel size: 83.92 Å; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
DOI: 10.6075/j05b02t1
title: CIL:57272. In Cell Image Library

description: Pixel size: 8.25 Å ; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
description: Ebola virus Huh7 infected cells 18 hours post infection, anti-nucleocapsid immuno-golg labelling.
DOI: 10.6075/j0ws8tmg
title: CIL:57267. In Cell Image Library

description: Ebola virus Huh7 infected cells 18 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
description: Pixel size: 8.25 Å ; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
DOI: 10.6075/j0n87b4p
title: CIL:57262. In Cell Image Library

description: Ebola virus Huh7 infected cells 18 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
description: Pixel size: 8.25 Å ; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
DOI: 10.6075/j0dr2vv2
title: CIL:57233, Ebola virus - Mayinga, Zaire, 1976, Huh7. In Cell Image Library

description: Ebola virus Huh7 infected cells 14 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Pixel size: 83.92 Å; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
description: Imaging mode: transmission electron microscopy (TEM); thin-section
DOI: 10.6075/j02j6c63
title: CIL:57231, Ebola virus - Mayinga, Zaire, 1976, Huh7. In Cell Image Library

description: Imaging mode: transmission electron microscopy (TEM); thin-section
description: Ebola virus Huh7 infected cells 14 hours post infection, anti-nucleocapsid immuno-golg labelling.
description: Pixel size: 83.92 Å; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
DOI: 10.6075/j0xs5vrv
title: CIL:57227, Ebola virus - Mayinga, Zaire, 1976, Huh7. In Cell Image Library

description: Imaging mode: transmission electron microscopy (TEM); thin-section
description: Pixel size: 83.92 Å; image size: 2048x2048; Samples were chemically fixed, high-pressure frozen and freeze substituted in HM20, thin sections 100 nm thickness.
description: Ebola virus Huh7 infected cells 14 hours post infection, anti-nucleocapsid immuno-golg labelling.
DOI: 10.6075/j0bz64ws
title: CIL:11372, Chinchilla, spermatid. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; embedded tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Data qualification: Processed
description: Figures 206 (upper) and 207 (lower) from Chapter 6 (Golgi Apparatus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. During development of the acrosome in the chinchilla spermatid, the transitional zone between the endoplasmic reticulum and the Golgi apparatus is particularly apparent. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0cv4gjz
title: CIL:11237, Macaca mulatta, Limulus polyphemus, non-pigmented ciliary epithelial cell, epithelial cell. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: Representative examples of gap junctions from vertebrates (ciliary epithelium from the eye of a Macaca mulatta, upper) and invertebrates (inverted gap junctions from cells of the mid-gut of the horseshoe crab, Limulus polyphemus, lower). Gap junctions in vertebrates consist of closely aggregated 8-9nm particles on the P-face, whereas those in invertebrate tissues have somewhat larger intramembranous particles associated with the E-face in freeze fracture preparations. Figures 104 (upper, by Giuseppina Raviola) and 105 (lower, from Lane, J. Cell Sci. 32: 293-305, 1978,reprinted with permission in Chapter 3 (Junctional Specializations) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0930s0s
title: CIL:11376, Rattus, hepatocyte. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; embedded tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Data qualification: Processed
description: Figure 209 from Chapter 6 (Golgi Apparatus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Portions of two hepatic cells and the intervening bile canaliculus from rat liver. Two stacks of Golgi cisternae near the bile canaliculus contain vacuoles with multiple very low density lipoprotein particles at the secretory face (arrows). Image by Robert Bolender. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0bg2msw
title: CIL:11384, Cavia porcellus, Rattus, Leydig cell, acinar cell. In Cell Image Library

description: Figures 213 (upper) and 214 (lower) from Chapter 6 (Golgi Apparatus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. The Golgi complex of a Leydig cell from guinea pig testis (upper) and from and acinar cell from rat pancreas (lower). Content of the Golgi lumen has been extracted from the steroid-secreting Leydig cell, whereas it has been preserved in the protein-secreting acinar cell and appears as a dense, granular particulate. Image in the lower panel by Daniel Friend. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; embedded tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Data qualification: Processed