DOI: 10.5061/dryad.dncjsxmcb
title: Data from: Neuronal migration depends on blood flow in the adult mammalian brain

description: In animal tissues, several cell types migrate along blood vessels, raising
 the possibility that blood flow influences cell migration. Here, we show
 that blood flow promotes the migration of new olfactory-bulb neurons in
 the adult brain. Neuronal migration is facilitated by blood flow, leading
 to the accumulation of new neurons near blood vessels with abundant blood
 flow. Blood flow inhibition attenuates blood vessel-guided neuronal
 migration, suggesting that blood contains factors beneficial to neuronal
 migration. We found that ghrelin, which is increased in the blood by
 hunger, directly influences neuronal migration. Ghrelin signaling promotes
 somal translocation by activating actin cytoskeleton contraction at the
 rear of the cell soma. New neurons mature in the olfactory bulb and
 contribute to the olfactory function for sensing odorants from food.
 Finally, we show that neuronal migration is increased by calorie
 restriction and that ghrelin signaling is involved in the process. This
 study suggests that blood flow promotes neuronal migration through
 blood-derived ghrelin signaling in the adult brain, which could be one of
 the mechanisms that improve the olfactory function for food-seeking
 behavior during starvation.
description: # Data from: Neuronal migration depends on blood flow in the adult
 mammalian brain Dataset DOI:
 [10.5061/dryad.dncjsxmcb](https://doi.org/10.5061/dryad.dncjsxmcb) ##
 Description of the data and file structure We performed in vitro and in
 vivo experiments by immunohistochemistry and fluorescent live imaging. ###
 Files and variables #### File: p_value.csv Description: p-value from all
 data #### File: Figure5-figure_supplement_4.csv Description: Proportion of
 NeuN+/Dcx- cells among total BrdU+ cells in the OB in the ad libitum (AL)
 and 70% calorie restriction (CR) groups #### File:
 Figure5-figure_supplement_3B.csv Description: Proportion of Ghsr1a-KD
 cells in the OB in the Sham and bilateral carotid artery stenosis (BCAS)
 groups analyzed by immunohistochemistry in 6 to 12-week-old adult mice
 #### File: Figure5-figure_supplement_2.csv Description: Quantification of
 Ki67+ cells in the control/ghsr1a-KD groups in vivo #### File:
 Figure5-figure_supplement_1.csv Description: Analysis of migration speed
 in control/ghsr1a-KD new neurons in vivo #### File:
 Figure5-figure_supplement_3C.csv Description: Normalized density of
 Control/Ghsr1a-KD in perivascular regions of endomucin-positive/-negative
 vessels in the RMS in the Sham and bilateral carotid artery stenosis
 (BCAS) groups analyzed by immunohistochemistry in 6 to 12-week-old adult
 mice #### File: Figure3-figure_supplement_1.csv
 Description: Quantification of Ghsr1a mRNA puncta per Dcx+ cell in the OB
 of the AL and CR mice #### File: Figure2-figure_supplement_1.csv
 Description: Analysis of the effect of bilateral carotid artery stenosis
 groups (BCAS) on cell proliferation and survival in the RMS and VSVZ,
 analyzed by immunohistochemistry in 6 to 12-week-old adult mice #### File:
 Figure1-figure_supplement_2H.csv Description: Density of BrdU+/Dcx+ cells
 in the vicinity of endomucin-positive and endomucin-negative vessels in
 the GL, GCL, and RMS was analyzed by immunohistochemistry in 6 to
 12-week-old adult mice #### File: Figure1-figure_supplement_3.csv
 Description: Density of immature neurons in the vicinity of
 SLC16A1-positive and -negative vessels in the mice and common marmosets
 brain #### File: Figure_5.csv Description: Analysis of migration speed in
 control/ghsr1a-KD new neurons in vivo #### File: Figure4D-J.csv
 Description: Analysis of migration speed, percentage of migratory phase,
 migration cycle, length/speed of leading process extension, and
 stride/speed of somal translocation in neuronal migration in vitro ####
 File: Figure1-figure_supplement_2E-G.csv Description: Density of
 BrdU+/Dcx+ cells in the vicinity of endomucin-positive and
 endomucin-negative vessels in the GL, GCL, and RMS was analyzed by
 immunohistochemistry in 6 to 12-week-old adult mice #### File:
 Figure1-figure_supplement_2C.csv Description: Average RBC flow in
 endomucin-positive and endomucin-negative vessels analyzed by two-photon
 imaging and immunohistochemistry in 6 to 12-week-old adult mice #### File:
 Figure3D.csv Description: Normalized fluorescence signal intensity in
 vascular endothelial cells analyzed by immunohistochemistry in 6 to
 12-week-old adult mice #### File: Figure4B.csv Description: Analysis for
 examining the effect of ghrelin on neuronal migration in vitro #### File:
 Figure1P.csv Description: Density of newly added neurons in the
 perivascular region analyzed by two-photon imaging in 6 to 12-week-old
 adult VGAT-Venus mice, in which GABAergic neurons are labeled with Venus
 #### File: Figure2G__H.csv Description: Comparison of migration speed
 before and after laser irradiation in the control (G)  and photothrombosis
 groups (H), analyzed by two-photon imaging in 6 to 12-week-old adult
 Dcx-EGFP mice #### File: Figure3F.csv Description: Comparison of signal
 gradient in extra-vessel regions analyzed by immunohistochemistry in 6 to
 12-week-old adult mice #### File: Figure1N.csv Description: Density of
 BrdU+/Dcx+ cells in the perivascular region of arteriole-side and
 venule-side capillaries analyzed by immunohistchemistry in NG2-DsRed mice
 #### File: Figure1K.csv Description: Maximum migration speed of new
 neurons analyzed by two-photon imaging in 6 to 12-week-old adult mice ####
 File: Figure1-figure_supplement_1.csv Description: Classification of
 neuron-vessel interaction analyzed by two-photon imaging in 6 to
 12-week-old adult Dcx-EGFP mice #### File: Figure1J.csv
 Description: Percentage of migratory period analyzed by two-photon imaging
 in 6 to 12-week-old adult mice #### File: Figure1I.csv
 Description: Average migration speed analyzed by two-photon imaging in 6
 to 12-week-old adult mice #### File: Figure1H.csv Description: Density of
 perivascular migrating cells analyzed by two-photon imaging in 6 to
 12-week-old adult mice #### File: Figure4L__M.csv Description: Analysis of
 the duration of actin cups and migration distance during actin cup
 formation in new neurons in vitro #### File: Figure1D.csv
 Description: Distance between new neurons and nearest vessels in the
 olfactory bulb and RMS analyzed by three-dimensional imaging in 6 to
 12-week-old adult mice #### File: Figure1G.csv Description: Average
 distance between migrating cells and nearest blood vessels analyzed by
 two-photon imaging in 6 to 12-week-old adult mice #### File: Figure2C.csv
 Description: Proportion of lentivirally-labeled cells in the OB in the
 Sham and bilateral carotid artery stenosis groups analyzed by
 immunohistochemistry in 6 to 12-week-old adult mice