cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0st7pv0
title: CIL:12559, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dissociated postmitotic neuron grown in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Presynaptic contacts are abundant on both cell bodies and the dendritic arbor of cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (green) and synapsin I (red), a presynaptic vesicle protein. With the exception of the immunostained presynaptic terminals, axons are not visible in this preparation. A relatively low power objective (20X) was used to capture the entire dendritic arbor of individual cells.
Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (monoclonal HM2, Sigma, with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and synapsin I (from P. DeCamilli, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]). Images were acquired with a Leica DMRA microscope with a 20X (HC Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Individual images of each fluorophore were colorized and assembled as a a stack file using MetaMorph software.
DOI: 10.6075/j00c4vsd
title: CIL:12558, Rattus, multipolar neuron. In Cell Image Library

description: Presynaptic contacts are abundant on both cell bodies and the dendritic arbor of cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (green) and synapsin I (red), a presynaptic vesicle protein. With the exception of the immunostained presynaptic terminals, axons are not visible in this preparation. A relatively low power objective (20X) was used to capture the entire dendritic arbor of individual cells.
Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (monoclonal HM2, Sigma, with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and synapsin I (from P. DeCamilli, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]). Images were acquired with a Leica DMRA microscope with a 20X (HC Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Individual images of each fluorophore were colorized and assembled as a a stack file using MetaMorph software.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dissociated postmitotic neuron grown in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0542npz
title: CIL:12557, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dissociated postmitotic neuron grown in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Presynaptic contacts are abundant on both cell bodies and the dendritic arbor of cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (green) and synapsin I (red), a presynaptic vesicle protein. With the exception of the immunostained presynaptic terminals, axons are not visible in this preparation. A relatively low power objective (20X) was used to capture the entire dendritic arbor of individual cells.
Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (monoclonal HM2, Sigma, with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and synapsin I (from P. DeCamilli, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]). Images were acquired with a Leica DMRA microscope with a 20X (HC Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Individual images of each fluorophore were colorized and assembled as a a stack file using MetaMorph software.
DOI: 10.6075/j0vh5nx7
title: CIL:12555, Rattus, multipolar neuron. In Cell Image Library

description: Presynaptic contacts are abundant on both cell bodies and the dendritic arbor of cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (green) and synapsin I (red), a presynaptic vesicle protein. With the exception of the immunostained presynaptic terminals, axons are not visible in this preparation. A relatively low power objective (20X) was used to capture the entire dendritic arbor of individual cells.
Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (monoclonal HM2, Sigma, with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and synapsin I (from P. DeCamilli, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]). Images were acquired with a Leica DMRA microscope with a 20X (HC Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Individual images of each fluorophore were colorized and assembled as a a stack file using MetaMorph software.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dissociated postmitotic neuron grown in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0qv3kmt
title: CIL:12595, Trypanosoma brucei, cell by organism, eukaryotic cell, Eukaryotic Protist, Flagellated Protist. In Cell Image Library

description: The flagellar pocket of a Trypanosoma brucei bloodstream form cell, with short flagellum. The image shows a new flagellum starting to grow from the flagellar pocket, in preparation for the next cell division. Cells were high pressure frozen and freeze substituted (1hr freeze substitution in 2% uranyl acetate in acetone and 8% methanol). An 80nm thick section was stained for 5 minutes with uranyl acetate, one minute with lead citrate and viewed using a Philips CM100 transmission electron microscope. The image was captured with an Advantage HS-B camera (2000 x 2000 pixels; AMT, Danvers, MA, USA). Contrast adjusted using Photoshop. Magnification indicated by scale bar.
description: Preparation: microtome-sectioned tissue; freeze substitution
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; electron bombardment CCD (EBCCD)
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt
Processing history: recorded image; electron bombardment CCD (EBCCD); Contrast enhanced in Photoshop.
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0vt1qsv
title: CIL:12617, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: High resolution image of an early endosome in planar view that contains HRP-reaction product. This cell was exposed to HRP for 6 min. and then fixed. This early endosome had two small coated evaginations arising from its rim and also had two large HRP-containing uncoated vesicles docked next to it presumably ready to fuse with it (for this picture and a serial section showing these two vesicles and the second evagination see Fig. 14C in Allen, Schroeder and Fok, J. Cell Sci. 101:449-461, 1992). Mitochondria have an endogenous peroxidase activity. This image was adapted with permission from Fig. 14. TEM taken on 10/20/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt; horseradish peroxidase
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.15468/dl.vtqfpt
title: Occurrence Download

description: A dataset containing 64931307 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is India",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 64931307 records from 3466 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009380-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5517/cc43mvp
title: CCDC 122662: Experimental Crystal Structure Determination

description: Related Article: F.Toda, K.Tanaka, H.Miyamoto, H.Koshima, I.Miyahara, K.Hirotsu|1997|J.Chem.Soc.,Perkin Trans.2||1877|doi:10.1039/a606561i
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k1zp
title: CCDC 135562: Experimental Crystal Structure Determination

description: Related Article: T.Funaioli, F.Marchetti, G.Fachinetti|1999|Chem.Commun.||2043|doi:10.1039/a905798f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k20r
title: CCDC 135563: Experimental Crystal Structure Determination

description: Related Article: T.Funaioli, F.Marchetti, G.Fachinetti|1999|Chem.Commun.||2043|doi:10.1039/a905798f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc46tvz
title: CCDC 125731: Experimental Crystal Structure Determination

description: Related Article: M.J.Hannon, C.L.Painting, N.W.Alcock|1999|Chem.Commun.||2023|doi:10.1039/a905795a
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4jx5q
title: CCDC 135413: Experimental Crystal Structure Determination

description: Related Article: J.Kowalik, D.VanDerveer, C.Clower, L.M.Tolbert|1999|Chem.Commun.||2007|doi:10.1039/a905940g
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k1xm
title: CCDC 135560: Experimental Crystal Structure Determination

description: Related Article: D.S.Wragg, I.Bull, G.B.Hix, R.E.Morris|1999|Chem.Commun.||2037|doi:10.1039/a906544j
description: Related Article: D.S.Wragg, A.M.Z.Slawin, R.E.Morris|2001|J.Mater.Chem.|11|1850|doi:10.1039/b100471i
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4jx4p
title: CCDC 135412: Experimental Crystal Structure Determination

description: Related Article: M.Ohkita, T.Suzuki, T.Tsuji|1999|Chem.Commun.||1999|doi:10.1039/a906239d
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4jx6r
title: CCDC 135414: Experimental Crystal Structure Determination

description: Related Article: J.Kowalik, D.VanDerveer, C.Clower, L.M.Tolbert|1999|Chem.Commun.||2007|doi:10.1039/a905940g
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k23v
title: CCDC 135566: Experimental Crystal Structure Determination

description: Related Article: U.Abram, D.Belli Dell'Amico, F.Calderazzo, C.Della Porta, U.Englert, F.Marchetti, A.Merigo|1999|Chem.Commun.||2053|doi:10.1039/a904792a
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k24w
title: CCDC 135567: Experimental Crystal Structure Determination

description: Related Article: U.Abram, D.Belli Dell'Amico, F.Calderazzo, C.Della Porta, U.Englert, F.Marchetti, A.Merigo|1999|Chem.Commun.||2053|doi:10.1039/a904792a
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc3jm8h
title: CCDC 105346: Experimental Crystal Structure Determination

description: Related Article: N.Burzlaff, W.A.Schenk|1999|Eur.J.Inorg.Chem.|1999|1435|doi:10.1002/(SICI)1099-0682(199909)1999:9<1435::AID-EJIC1435>3.3.CO;2-L
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc3v4jl
title: CCDC 114499: Experimental Crystal Structure Determination

description: Related Article: A.Salzer, A.Hosang, J.Knuppertz, U.Englert|1999|Eur.J.Inorg.Chem.|1999|1497|doi:10.1002/(SICI)1099-0682(199909)1999:9<1497::AID-EJIC1497>3.3.CO;2-Y
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k25x
title: CCDC 135568: Experimental Crystal Structure Determination

description: Related Article: U.Abram, D.Belli Dell'Amico, F.Calderazzo, C.Della Porta, U.Englert, F.Marchetti, A.Merigo|1999|Chem.Commun.||2053|doi:10.1039/a904792a
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k2vl
title: CCDC 135589: Experimental Crystal Structure Determination

description: Related Article: D.Bellamy, N.C.Brown, N.G.Connelly, A.G.Orpen|1999|J.Chem.Soc.,Dalton Trans.||3191|doi:10.1039/a902481f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k32v
title: CCDC 135596: Experimental Crystal Structure Determination

description: Related Article: L.King, M.Motevalli, A.C.Sullivan|1999|J.Chem.Soc.,Dalton Trans.||3225|doi:10.1039/a904429i
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k33w
title: CCDC 135597: Experimental Crystal Structure Determination

description: Related Article: L.King, M.Motevalli, A.C.Sullivan|1999|J.Chem.Soc.,Dalton Trans.||3225|doi:10.1039/a904429i
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k34x
title: CCDC 135598: Experimental Crystal Structure Determination

description: Related Article: L.King, M.Motevalli, A.C.Sullivan|1999|J.Chem.Soc.,Dalton Trans.||3225|doi:10.1039/a904429i
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k35y
title: CCDC 135599: Experimental Crystal Structure Determination

description: Related Article: A.Houlton, C.J.Isaac, A.E.Gibson, B.R.Horrocks, W.Clegg, M.R.J.Elsegood|1999|J.Chem.Soc.,Dalton Trans.||3229|doi:10.1039/a905168f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k36z
title: CCDC 135600: Experimental Crystal Structure Determination

description: Related Article: A.Houlton, C.J.Isaac, A.E.Gibson, B.R.Horrocks, W.Clegg, M.R.J.Elsegood|1999|J.Chem.Soc.,Dalton Trans.||3229|doi:10.1039/a905168f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc4k370
title: CCDC 135601: Experimental Crystal Structure Determination

description: Related Article: A.Houlton, C.J.Isaac, A.E.Gibson, B.R.Horrocks, W.Clegg, M.R.J.Elsegood|1999|J.Chem.Soc.,Dalton Trans.||3229|doi:10.1039/a905168f
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.