DOI: 10.5061/dryad.7h44j0zrd
title: Belowground fungi, soil, and root chemistry in tropical landuse systems

description: The data were collected in the humid tropical climate on Sumatra
 (Indonesia) in rain forests, in jungle rubber (rubber planted into
 forests), in rubber, and oil palm plantations. A total of 44 plots were
 sampled. The data set consists of two tables. The table Environmental_data
 contains plot information, geographic coordinates of the plots, and data
 on soil properties (pH, nitrogen, carbon, C/N, potassium, calcium,
 magnesium, manganese, iron, phosporous, soil resource index, soil PC1,
 soil PC2) and on root traits (biomass, nitrogen, carbon, C/N, potassium,
 calcium, magnesium, manganese, iron, phosporous, root resource index, root
 PC1, soil PC, root vitality). Table 2 contain abundance data (OTU counts)
 for fungi associated with roots or with soil and their phylogenetic and
 functional assignments.
description: Sampling: Root and soil
 were collected in 44 plots in different landuse systems on Sumatra. In
 each plot three replicates were sampled and analysed individually.
 Environmental_data are means of n = 3 per plot. Fungal_data contain the
 sum of the OTU counts of three plots. Environmental_data: Soil and root
 tissue were dried and milled. Carbon and nitrogen were determined in a C/N
 analyzer (Vario MICRO analyzer, Elementar, Langensbold, Germany). Other
 elements were extracted  in 65% HNO<sub>3</sub> in a digestion
 microwave (Ethos Start, Milestone, Sorisole, Italy) for 30 min at 210 ℃
 and measured using inductively coupled plasma mass spectrometry (ICP-OES,
 iCap 7000, Thermo Fisher Scientific, Waltham, USA). pH was determined in
 0.1 M KCl. Root tips were inspected under a microscope and classified as
 vital or dead. Fungal_data: DNA was extracted
 from freeze-dried, milled soil and root samples using kits (uPREP Plant
 DNA kits Analytik Jena AG, Jena, Germany, DNeasy PowerSoil Kit Qiagen,
 Venlo, Netherlands). For amplication of the fungal ITS1 marker region, the
 ITS1f-KYO2 and ITS2 primer pair with specific overhang adapters for
 Illumina sequencing were used. Amplicon sequencing and amplicon sequence
 variants (ASV) assembly were conducted on the MiSeq platform using the
 MiSeq Reagent Kit v3 (Illumina Inc., San Diego, USA). OTU clustering
 occurred at a 97% similarity threshold. Reads were mapped to the library
 and used to generate a count table. The count table was rarefied. OTUs
 were taxonomically classified using the BLAST algorithm against the UNITE
 v.7.2 public database. OTUs were functionally annotated as symbiotroph,
 pathotroph, and saprotroph using the FUNGuild public database.
description: <strong>Abbreviations</strong>: H = harapan landscape; B = bukit landscape; F = forest; J = jungle-rubber; R = rubber plantation; O = oil palm plantation; c = core plot (well drained soil); r = riparian site. <strong>Units: </strong>Elements are indicated in the units: mg g<sup>-1</sup> dry mass of soil or dry mass of roots. Root biomass (g m<sup>-2</sup> to a depth of 0.15 m), Vitality (% of vital root tips per total root tips). <strong>Table Environmental_data</strong>: Landscape, land-use type and coordinates of all research plots as well as all measured soil chemical properties and root community traits. RI = Resource Index (sum of standardized calcium, magnesium, potassium, iron and manganese concentrations), PC = principle component axis scores from the principle component analysis for soil and roots. <strong>Table Fungal_data</strong>: Read counts of fungal operational taxonomic units (OTU; clustered at 97 % similarity threshold) in soil and root samples of each plot (columns 1 – 89) as well as taxonomic (columns 90 – 92) and functional (column 93 – 98) classification;