cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11310854.v2
title: A phytosociological analysis of the <i>Olea europaea</i> L. var. <i>sylvestris</i> (Mill.) Lehr. forests in Sicily

description: This paper presents a phytosociological study on the forest vegetation of <i>Olea europaea</i> var. <i>sylvestris</i> of Sicily and of the smaller minor islands. In Sicily, Oleaster formations show considerable climacic potentiality in the bioclimactic belts between the infra- and the thermomediterranean with single edapho-climacic penetrations that are also in the mesomediterranean; however, these were largely destroyed by man in order to make room for crops. Furthermore, the residual expressions of the Oleaster forests are limited, and often exist as regenerated woodland made possible by the abandonment of agricultural land; they are often small forest nuclei – high maquis, woods and micro-woods – with a more or less discontinuous distribution within the agricultural landscape. The fieldwork is supported by 120 phytosociological surveys carried out in various areas of Sicily, which were statistically analyzed and compared with other published data from surveys of other communities that have been described for the Tyrrhenian (Sardinia and Corsica) and Balearic Islands up until now. The phytosociological and statistical analysis has led to the description of three new associations two of which are classified in the <i>Oleo-Ceratonion</i> alliance (<i>Ruto chalepensis-Oleetum sylvestris</i> and <i>Chamaeropo humilis-Oleetum sylvestris</i>), the third in the <i>Erico-Quercion ilicis</i> alliance (<i>Calicotomo infestae-Oleetum sylvestris</i>).
DOI: 10.6084/m9.figshare.5634802.v1
title: Radio telescope construction for analysis of 12 GHz solar microwaves

description: This work deals the construction of a radio telescope, its characteristics and functioning. All the equipment, materials, software and adjustments necessary are specified step by step for the analysis of solar microwaves at the 12 GHz frequency. In the prototype construction, materials of easy acquisition and relatively low cost were used in order to have an affordable and versatile equipment. For the adjustments and calibration of the assembled prototype, the radiation emitted by a fluorescent lamp was measured in a closed environment. In the second test the measurement of the solar transit was made. The equipment showed up to be easy to assemble and calibration, allowing its use in the classroom, laboratory and in open environments for the detection of solar radiation and other celestial bodies, as well as for the discussion of different physical concepts.
DOI: 10.6084/m9.figshare.10322096
title: Identification of the areas of endemism (AOEs) of the genus <i>Acantholimon</i> (Plumbaginaceae) in Iran

description: This study was conducted to identify areas of endemism for <i>Acantholimon</i> species using parsimony analysis of endemicity (PAE) and to detect endemic species richness of the genus in the region. The results obtained from the two methods used in this study were used in determining the priorities for the conservation of <i>Acantholimon</i> species in Iran. The distribution database of 62 endemic species belonging to this genus was formed by 1250 georeferenced observations in Iran. The study area was divided into 1̊×1̊ grids of operative geographical units (OGUs) and the species × area matrix including presence/absence data was created. The endemic species richness was calculated using circular neighborhood with a radius of 50 km in 10 × 10 km<sup>2</sup> raster cells using DIVA-GIS software. The results of PAE analysis have shown four areas of endemism (AOEs) in Iran. AOE1: including Alborz and Zagros mountains, the mountains of central Iran. AOE2 and AOE3 are located in Khorassan subregion and AOE4 contains parts of western Iran. The map of endemic species richness indicated that the highest number of endemic species occurs in central Alborz region as well as Kerman, Chahar-Mahal and Bakhtiari, and Isfahan provinces.
DOI: 10.6084/m9.figshare.5634799
title: Effect of temperature and nitrogen concentration on biomass composition of Heterochlorella luteoviridis

description: Abstract The interest in microalga as a food supplement has grown due their high contents of carotenoids, polyunsaturated fatty acids and proteins. This study evaluated the effect of different temperatures (22, 27 or 32 °C) and sodium nitrate concentrations (12, 24, 36, 48 or 60 mg L-1 of N-NO3) in culture medium on Heterochlorella luteoviridis biomass production and composition. The highest biomass concentration (3.35 g L-1) was observed at the highest N-NO3 concentration. The N-NO3 concentration positively affected protein, carbohydrate and carotenoids contents of biomass. On the other hand, cells cultured at the lowest N-NO3 concentration showed a slight increment in lipid content. The major carotenoid was lutein (30.7 ± 1.4% of total carotenoids), and the polyunsaturated fatty acids were 37 ± 2% of total fatty acids. Low temperature improved the biosynthesis of ω3 type fatty acids by lowering the ω6:ω3 ratio. Overall, our results indicate H. luteoviridis can yield high biomass concentration under autotrophic growth, resulting in a biomass rich in carotenoids, mainly lutein, and ω3 polyunsaturated fatty acids.
DOI: 10.6084/m9.figshare.11310854.v1
title: A phytosociological analysis of the <i>Olea europaea</i> L. var. <i>sylvestris</i> (Mill.) Lehr. forests in Sicily

description: This paper presents a phytosociological study on the forest vegetation of <i>Olea europaea</i> var. <i>sylvestris</i> of Sicily and of the smaller minor islands. In Sicily, Oleaster formations show considerable climacic potentiality in the bioclimactic belts between the infra- and the thermomediterranean with single edapho-climacic penetrations that are also in the mesomediterranean; however, these were largely destroyed by man in order to make room for crops. Furthermore, the residual expressions of the Oleaster forests are limited, and often exist as regenerated woodland made possible by the abandonment of agricultural land; they are often small forest nuclei – high maquis, woods and micro-woods – with a more or less discontinuous distribution within the agricultural landscape. The fieldwork is supported by 120 phytosociological surveys carried out in various areas of Sicily, which were statistically analyzed and compared with other published data from surveys of other communities that have been described for the Tyrrhenian (Sardinia and Corsica) and Balearic Islands up until now. The phytosociological and statistical analysis has led to the description of three new associations two of which are classified in the <i>Oleo-Ceratonion</i> alliance (<i>Ruto chalepensis-Oleetum sylvestris</i> and <i>Chamaeropo humilis-Oleetum sylvestris</i>), the third in the <i>Erico-Quercion ilicis</i> alliance (<i>Calicotomo infestae-Oleetum sylvestris</i>).
DOI: 10.6084/m9.figshare.5634799.v1
title: Effect of temperature and nitrogen concentration on biomass composition of Heterochlorella luteoviridis

description: Abstract The interest in microalga as a food supplement has grown due their high contents of carotenoids, polyunsaturated fatty acids and proteins. This study evaluated the effect of different temperatures (22, 27 or 32 °C) and sodium nitrate concentrations (12, 24, 36, 48 or 60 mg L-1 of N-NO3) in culture medium on Heterochlorella luteoviridis biomass production and composition. The highest biomass concentration (3.35 g L-1) was observed at the highest N-NO3 concentration. The N-NO3 concentration positively affected protein, carbohydrate and carotenoids contents of biomass. On the other hand, cells cultured at the lowest N-NO3 concentration showed a slight increment in lipid content. The major carotenoid was lutein (30.7 ± 1.4% of total carotenoids), and the polyunsaturated fatty acids were 37 ± 2% of total fatty acids. Low temperature improved the biosynthesis of ω3 type fatty acids by lowering the ω6:ω3 ratio. Overall, our results indicate H. luteoviridis can yield high biomass concentration under autotrophic growth, resulting in a biomass rich in carotenoids, mainly lutein, and ω3 polyunsaturated fatty acids.
DOI: 10.6084/m9.figshare.5634796
title: Didatic approach of ultrasonographic examination for evaluation of the carpal joint in horses

description: ABSTRACT: The carpus is a complex articulation, which is often involved in injuries in equine athletes. The objective of this study was to suggest a didactic approach for ultrasonography training for the examination of the carpal joint in horses. Ultrasonograhy examination was performed in a healthy 14-year-old horse. The images were compared with those of a dissected anatomic specimen of the carpal region of a senior horse and with those reported in the literature. Identifiable structures were as follows: (dorsal) tendon of the muscle extensor carpi radialis, tendon of the muscle extensor carpi obliquus, tendon of the muscle commom digital extensor, dorsal synovial outpouchings, joint capsule and fat cushion, (lateral) tendon of the muscle lateral digital extensor, lateral collateral ligament (deep and superficial components), (medial) medial collateral ligament (deep and superficial components) and (palmar) palmar intercarpal ligament, carpal sheath, carpal tunnel, superficial digital flexor tendon, proximal origin of the deep digital flexor tendon, and palmar carpal ligament. Prior knowledge of the anatomy in combination with the study of anatomical specimens is essential for recognizing musculoskeletal structures during ultrasound examination, contributing to the training and learning processes.
DOI: 10.6084/m9.figshare.5634796.v1
title: Didatic approach of ultrasonographic examination for evaluation of the carpal joint in horses

description: ABSTRACT: The carpus is a complex articulation, which is often involved in injuries in equine athletes. The objective of this study was to suggest a didactic approach for ultrasonography training for the examination of the carpal joint in horses. Ultrasonograhy examination was performed in a healthy 14-year-old horse. The images were compared with those of a dissected anatomic specimen of the carpal region of a senior horse and with those reported in the literature. Identifiable structures were as follows: (dorsal) tendon of the muscle extensor carpi radialis, tendon of the muscle extensor carpi obliquus, tendon of the muscle commom digital extensor, dorsal synovial outpouchings, joint capsule and fat cushion, (lateral) tendon of the muscle lateral digital extensor, lateral collateral ligament (deep and superficial components), (medial) medial collateral ligament (deep and superficial components) and (palmar) palmar intercarpal ligament, carpal sheath, carpal tunnel, superficial digital flexor tendon, proximal origin of the deep digital flexor tendon, and palmar carpal ligament. Prior knowledge of the anatomy in combination with the study of anatomical specimens is essential for recognizing musculoskeletal structures during ultrasound examination, contributing to the training and learning processes.
DOI: 10.6084/m9.figshare.5634793
title: Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

description: ABSTRACT: The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P&lt;0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P&lt;0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.
DOI: 10.6084/m9.figshare.5634793.v1
title: Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

description: ABSTRACT: The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P&lt;0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P&lt;0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.
DOI: 10.6084/m9.figshare.5634787
title: Physical and chemical characteristics of cheese bread, using fermented broken rice

description: Abstract Development of new food products, taking as raw material the subproducts obtained during industrial process become an economic and nutritious alternative, since these are usually discarded, caning be a significant nutritional source good. This research aimed to develop cheese bread using fermented broken rice instead of sour starch in four different concentrations (0%, 25%, 50%, 75% and 100%). After the development of formulations, was performed physics and chemicals characterization of products obtained, performing analysis of: proximate composition, dietary fiber, acidity, pH, ºBrix, total soluble sugars, reducing and sucrose. The increased formulations didn’t present significant differences, highlighting the average values of protein 7%, dietary fiber 9% and ash 1.9%. Broken rice, after fermentation process, becomes a profitable alternative instead of the sour starch on cheese breads, saving all the physical and chemical characteristics and being inexpensive.
DOI: 10.6084/m9.figshare.5634787.v1
title: Physical and chemical characteristics of cheese bread, using fermented broken rice

description: Abstract Development of new food products, taking as raw material the subproducts obtained during industrial process become an economic and nutritious alternative, since these are usually discarded, caning be a significant nutritional source good. This research aimed to develop cheese bread using fermented broken rice instead of sour starch in four different concentrations (0%, 25%, 50%, 75% and 100%). After the development of formulations, was performed physics and chemicals characterization of products obtained, performing analysis of: proximate composition, dietary fiber, acidity, pH, ºBrix, total soluble sugars, reducing and sucrose. The increased formulations didn’t present significant differences, highlighting the average values of protein 7%, dietary fiber 9% and ash 1.9%. Broken rice, after fermentation process, becomes a profitable alternative instead of the sour starch on cheese breads, saving all the physical and chemical characteristics and being inexpensive.
DOI: 10.6084/m9.figshare.5634781
title: Complete sequence and comparative analysis of the chloroplast genome of Plinia trunciflora

description: Abstract Plinia trunciflora is a Brazilian native fruit tree from the Myrtaceae family, also known as jaboticaba. This species has great potential by its fruit production. Due to the high content of essential oils in their leaves and of anthocyanins in the fruits, there is also an increasing interest by the pharmaceutical industry. Nevertheless, there are few studies focusing on its molecular biology and genetic characterization. We herein report the complete chloroplast (cp) genome of P. trunciflora using high-throughput sequencing and compare it to other previously sequenced Myrtaceae genomes. The cp genome of P. trunciflora is 159,512 bp in size, comprising inverted repeats of 26,414 bp and single-copy regions of 88,097 bp (LSC) and 18,587 bp (SSC). The genome contains 111 single-copy genes (77 protein-coding, 30 tRNA and four rRNA genes). Phylogenetic analysis using 57 cp protein-coding genes demonstrated that P. trunciflora, Eugenia uniflora and Acca sellowiana form a cluster with closer relationship to Syzygium cumini than with Eucalyptus. The complete cp sequence reported here can be used in evolutionary and population genetics studies, contributing to resolve the complex taxonomy of this species and fill the gap in genetic characterization.
DOI: 10.6084/m9.figshare.5634781.v1
title: Complete sequence and comparative analysis of the chloroplast genome of Plinia trunciflora

description: Abstract Plinia trunciflora is a Brazilian native fruit tree from the Myrtaceae family, also known as jaboticaba. This species has great potential by its fruit production. Due to the high content of essential oils in their leaves and of anthocyanins in the fruits, there is also an increasing interest by the pharmaceutical industry. Nevertheless, there are few studies focusing on its molecular biology and genetic characterization. We herein report the complete chloroplast (cp) genome of P. trunciflora using high-throughput sequencing and compare it to other previously sequenced Myrtaceae genomes. The cp genome of P. trunciflora is 159,512 bp in size, comprising inverted repeats of 26,414 bp and single-copy regions of 88,097 bp (LSC) and 18,587 bp (SSC). The genome contains 111 single-copy genes (77 protein-coding, 30 tRNA and four rRNA genes). Phylogenetic analysis using 57 cp protein-coding genes demonstrated that P. trunciflora, Eugenia uniflora and Acca sellowiana form a cluster with closer relationship to Syzygium cumini than with Eucalyptus. The complete cp sequence reported here can be used in evolutionary and population genetics studies, contributing to resolve the complex taxonomy of this species and fill the gap in genetic characterization.
DOI: 10.6084/m9.figshare.5634778
title: Validation of spectrophotometric microplate methods for polyphenol oxidase and peroxidase activities analysis in fruits and vegetables

description: Abstract Enzymes polyphenol oxidase (PPO) and peroxidase (POD) play important roles in the processing of fruits and vegetables, since they can produce undesirable changes in color, texture and flavor. Classical methods of activity assessment are based on cuvette spectrophotometric readings. This work aims to propose, to validate and to test microplate spectrophotometric methods. Samples of apple juice and lyophilized enzymes from mushroom and horseradish were analyzed by the cuvette and microplate methods and it was possible to validate the microplate assays with satisfactory results regarding linearity, repeatability, accuracy along with quantitation and detection limits. The proposed microplate methods proved to be reliable and reproducible as the classical methods besides having the advantages of allowing simultaneous analysis and requiring a reduced amount of samples and reactants, which can beneficial to the study of enzyme inactivation in the processing of fruits and vegetables.
DOI: 10.6084/m9.figshare.5634778.v1
title: Validation of spectrophotometric microplate methods for polyphenol oxidase and peroxidase activities analysis in fruits and vegetables

description: Abstract Enzymes polyphenol oxidase (PPO) and peroxidase (POD) play important roles in the processing of fruits and vegetables, since they can produce undesirable changes in color, texture and flavor. Classical methods of activity assessment are based on cuvette spectrophotometric readings. This work aims to propose, to validate and to test microplate spectrophotometric methods. Samples of apple juice and lyophilized enzymes from mushroom and horseradish were analyzed by the cuvette and microplate methods and it was possible to validate the microplate assays with satisfactory results regarding linearity, repeatability, accuracy along with quantitation and detection limits. The proposed microplate methods proved to be reliable and reproducible as the classical methods besides having the advantages of allowing simultaneous analysis and requiring a reduced amount of samples and reactants, which can beneficial to the study of enzyme inactivation in the processing of fruits and vegetables.
DOI: 10.6084/m9.figshare.5634775
title: Charles Miller Fisher: the 65th anniversary of the publication of his groundbreaking study “Transient Monocular Blindness Associated with Hemiplegia”

description: ABSTRACT Charles Miller Fisher is considered the father of modern vascular neurology and one of the giants of neurology in the 20th century. This historical review emphasizes Prof. Fisher's magnificent contribution to vascular neurology and celebrates the 65th anniversary of the publication of his groundbreaking study, “Transient Monocular Blindness Associated with Hemiplegia.”
DOI: 10.6084/m9.figshare.5634775.v1
title: Charles Miller Fisher: the 65th anniversary of the publication of his groundbreaking study “Transient Monocular Blindness Associated with Hemiplegia”

description: ABSTRACT Charles Miller Fisher is considered the father of modern vascular neurology and one of the giants of neurology in the 20th century. This historical review emphasizes Prof. Fisher's magnificent contribution to vascular neurology and celebrates the 65th anniversary of the publication of his groundbreaking study, “Transient Monocular Blindness Associated with Hemiplegia.”
DOI: 10.6084/m9.figshare.5634772
title: CURRENT CHALLENGES ON THE PRODUCTION AND USE OF CELLULOLYTIC ENZYMES IN THE HYDROLYSIS OF LIGNOCELLULOSIC BIOMASS

description: The second generation (2G) ethanol obtained from lignocellulosic biomass has been considered as a potential alternative source of energy for the transportation sector. However, the technologies to convert lignocellulose into fermentable sugars still face several challenges. One of the major challenges of this process is related to the cost of the cellulolytic enzymes. Thus, the development of bioprocesses for enzyme production and strategies to increase the final yield of the enzymatic hydrolysis reactions are required to ensure the economic feasibility of the biomass conversion process. In this review, it is discussed the progress related to fungal enzyme technology over the past few years with major emphasis on the biocatalysts needed for cellulose degradation as well as on cultivation strategies for the production of enzymes on-site. The potential of applying secretome analysis as a tool for the characterization of the enzymatic complexes and some limitations on the use of enzymatic cocktails in the saccharification step, such as the unproductive adsorption of enzymes onto lignin are also discussed in light of the recent literature.