cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7591379.v1
title: Performance of rapid tests compared to conventional tests used for HIV diagnosis

description: ABSTRACT Introduction: Early diagnosis is an important tool for strategies to fight human immunodeficiency virus (HIV) infection. Objective: The main objective of this study is to evaluate the comparability of the results of rapid tests (RT) used for the HIV diagnosis in relation to conventional molecular and serological tests in blood samples from a population of men who have sex with men (MSM), from 12 Brazilian capitals. Material and methods: 591 HIV-1 reactive test samples from 4176 MSM participants were submitted to Instituto Adolfo Lutz (IAL) for evaluation by conventional laboratory tests. From these samples with at least one RT reagent, 522 samples were analyzed, and in 493 (94.4%) the HIV positivity was confirmed, with 33% HIV-1 viral load above 5,000 copies/mL and 67% by the serological tests. A total of 336 (10%) samples with a non-reactive RT result were evaluated by standard serology, four (1.2%) tested positive for HIV. Results and conclusion: The results showed a high percentage of samples with confirmed HIV positivity in the conventional laboratory tests, as well as some non-reactive results that were confirmed positive, indicating some limitations of the RT single-step method. Therefore, the serological tests had a fundamental role in clarifying the diagnosis.
DOI: 10.6084/m9.figshare.7591373
title: Synthesis and Cytotoxicity Evaluation of a Series of 3-Alkenyl-2-Hydroxy-1,4-Naphthoquinones Obtained by an Efficient Knoevenagel Condensation

description: A modified and efficient Knoevenagel condensation procedure was developed to synthesize the title compounds using β-alanine and acetic acid as catalysts, showing good to excellent yields. We used lawsone with suitable aliphatic aldehydes including isobutyraldehyde, 3-methyl-butyraldehyde, 2-ethylbutyraldehyde, phenylacetaldehyde, 2-phenyl-propionaldehyde, among others. These compounds were submitted to cytotoxic screening against three tumor cell lines presenting good to excellent cytotoxic profiles.
DOI: 10.15468/dl.7d2fsm
title: Occurrence Download

description: A dataset containing 1760 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus macranthus Maxim."
 ]
}

 The dataset includes 1760 records from 93 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042011-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.j78ace
title: Occurrence Download

description: A dataset containing 34 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Laggera elatior R.E.Fr."
 ]
}

 The dataset includes 34 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042012-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8cjdx3
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Argentina)"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042013-240321170329656/datasets/export for details.
DOI: 10.15468/dl.g5548k
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus mongolicus V.I.Krecz. & Kuprian."
 ]
}

 The dataset includes 14 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042015-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ynb2dw
title: Occurrence Download

description: A dataset containing 317 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Justicia anselliana (Nees) T.Anderson"
 ]
}

 The dataset includes 317 records from 47 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042014-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6f9b2u
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Laggera lecomteana O.Hoffm. & Muschl."
 ]
}

 The dataset includes 4 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042016-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.25486951
title: <b>Massive heterogeneity revealed by comprehensive mapping of the adult mammalian forebrain neurogenic ependyma</b>

description: For single cell RNA sequencing dataset, the 10× Genomics platform was used to perform massive single cell mRNA profiling from the lateral walls of the lateral ventricle (Enface) of adult mouse. Only male C57BL/6 mice were used in the analyses, whole mount enface tissue were dissociated into single cells through our proprietary method developed based on published studies<sup>1</sup>. Ependymal cells with high CD133 protein expression were enriched by soft Fluorescence-activated cell sorting (FACS) using the WOLF cell sorter from Nano Collector Inc.. CD133 positive cells were labeled as “PE” cells and CD133 negative cells were labeled as “NC” cells. Both “PE” and “NC” cells were subjected to single-cell transcriptomic sequencing using the 10× Genomics platform. [1] Liu J, Wang J, Xu J, et al. Comprehensive investigations revealed consistent pathophysiological alterations after vaccination with COVID-19 vaccines. Cell Discov. 2021 Oct 26;7(1):99.
DOI: 10.6084/m9.figshare.25486951.v1
title: <b>Massive heterogeneity revealed by comprehensive mapping of the adult mammalian forebrain neurogenic ependyma</b>

description: For single cell RNA sequencing dataset, the 10× Genomics platform was used to perform massive single cell mRNA profiling from the lateral walls of the lateral ventricle (Enface) of adult mouse. Only male C57BL/6 mice were used in the analyses, whole mount enface tissue were dissociated into single cells through our proprietary method developed based on published studies<sup>1</sup>. Ependymal cells with high CD133 protein expression were enriched by soft Fluorescence-activated cell sorting (FACS) using the WOLF cell sorter from Nano Collector Inc.. CD133 positive cells were labeled as “PE” cells and CD133 negative cells were labeled as “NC” cells. Both “PE” and “NC” cells were subjected to single-cell transcriptomic sequencing using the 10× Genomics platform. [1] Liu J, Wang J, Xu J, et al. Comprehensive investigations revealed consistent pathophysiological alterations after vaccination with COVID-19 vaccines. Cell Discov. 2021 Oct 26;7(1):99.
DOI: 10.15468/dl.htzcd7
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Argentina)"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042018-240321170329656/datasets/export for details.
DOI: 10.15468/dl.8vptum
title: Occurrence Download

description: A dataset containing 40 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus panzerioides Popov"
 ]
}

 The dataset includes 40 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042017-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.kdqru5
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Justicia antsingensis Benoist"
 ]
}

 The dataset includes 4 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042019-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z58cuu
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Laggera squarrosa Oliv. & Hiern"
 ]
}

 The dataset includes 4 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042020-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.74bsmf
title: Occurrence Download

description: A dataset containing 5337 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Benin",
 "OccurrenceStatus is Present",
 "TaxonKey is Afzelia africana Sm. ex Pers.",
 "Year 2004-2024"
 ]
}

 The dataset includes 5337 records from 61 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042021-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qek43x
title: Occurrence Download

description: A dataset containing 29 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus persicus Boiss."
 ]
}

 The dataset includes 29 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042023-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.10885433
title: Problems with nanoparticle tracking analysis (NTA) of urine extracellular vesicles (uEVs)

description: Urinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein composition using fluorescence measurements. However, despite much interest in the clinical application of uEVs, their analysis using the NTA method is poorly described and requires meticulous sample preparation, experimental adjustment of instrument settings, and above all, an understanding of the limitations of the method. We present the problems encountered during analysis with possible solutions: the choice of sample dilution, the method of the presentation and comparison of results, photobleaching, and the adjustment of instrument settings for a specific analysis.

 

Figure 1. Expressions of specific markers CD63 in protein-standardized samples detected with Western blot analysis; anti-CD 63 (HPA010088, Sigma-Aldrich, Saint Louis, MO, USA, 1:1000); secondary antibodies conjugated to horseradish peroxidase (554021, BD Pharmingen (BD Biosciences, San Jose, CA, USA) 1:10000).

 

Nanoparticle-Tracking Analysis of uEVs. A NanoSight NS300 instrument (Malvern Panalytical, Malvern, UK) was used to determine the concentrations and sizes of the uEVs in the samples. The total number of extracellular vesicles was measured during the continuous flow of samples delivered from a syringe pump.

Figure 2. Determination of the size and concentration of uEVs: dilution factor—1:100; laser—405 nm.

Figure 3. Effect of dilution on total number of particles per milliliter and size of uEVs in nanoparticle tracking analysis: sample dilutions—1:100, 1:500, and 1:1000; laser—488 nm.

Figure 5. Fluorescence-based nanoparticle-tracking analysis of CD 63 expression in uEVs: without 500 nm long-pass filter; with 500 nm long-pass filter; comparison of sizes and concentrations of uEVs without and with 500 nm long-pass filter; dilution factor—1:100; laser—488 nm; anti-CD 63 (HPA010088, Sigma-Aldrich); secondary antibodies conjugated to Alexa Fluor 488 fluorescent dye (ab150073-500, Abcam, Cambridge, MA, USA).

Figure 6. Fluorescence-based nanoparticle-tracking analysis of podocin expression in uEVs: without 500 nm long-pass filter; with 500 nm long-pass filter; comparison of sizes and concentrations of uEVs without and with 500 nm long-pass filter; dilution factor—1:100; laser—488 nm; anti-podocin (P0372, Sigma-Aldrich); secondary antibodies conjugated to Alexa Fluor 488 fluorescent dye (ab150073-500, Abcam, Cambridge, MA, USA).
DOI: 10.5281/zenodo.10885434
title: Problems with nanoparticle tracking analysis (NTA) of urine extracellular vesicles (uEVs)

description: Urinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein composition using fluorescence measurements. However, despite much interest in the clinical application of uEVs, their analysis using the NTA method is poorly described and requires meticulous sample preparation, experimental adjustment of instrument settings, and above all, an understanding of the limitations of the method. We present the problems encountered during analysis with possible solutions: the choice of sample dilution, the method of the presentation and comparison of results, photobleaching, and the adjustment of instrument settings for a specific analysis.

 

Figure 1. Expressions of specific markers CD63 in protein-standardized samples detected with Western blot analysis; anti-CD 63 (HPA010088, Sigma-Aldrich, Saint Louis, MO, USA, 1:1000); secondary antibodies conjugated to horseradish peroxidase (554021, BD Pharmingen (BD Biosciences, San Jose, CA, USA) 1:10000).

 

Nanoparticle-Tracking Analysis of uEVs. A NanoSight NS300 instrument (Malvern Panalytical, Malvern, UK) was used to determine the concentrations and sizes of the uEVs in the samples. The total number of extracellular vesicles was measured during the continuous flow of samples delivered from a syringe pump.

Figure 2. Determination of the size and concentration of uEVs: dilution factor—1:100; laser—405 nm.

Figure 3. Effect of dilution on total number of particles per milliliter and size of uEVs in nanoparticle tracking analysis: sample dilutions—1:100, 1:500, and 1:1000; laser—488 nm.

Figure 5. Fluorescence-based nanoparticle-tracking analysis of CD 63 expression in uEVs: without 500 nm long-pass filter; with 500 nm long-pass filter; comparison of sizes and concentrations of uEVs without and with 500 nm long-pass filter; dilution factor—1:100; laser—488 nm; anti-CD 63 (HPA010088, Sigma-Aldrich); secondary antibodies conjugated to Alexa Fluor 488 fluorescent dye (ab150073-500, Abcam, Cambridge, MA, USA).

Figure 6. Fluorescence-based nanoparticle-tracking analysis of podocin expression in uEVs: without 500 nm long-pass filter; with 500 nm long-pass filter; comparison of sizes and concentrations of uEVs without and with 500 nm long-pass filter; dilution factor—1:100; laser—488 nm; anti-podocin (P0372, Sigma-Aldrich); secondary antibodies conjugated to Alexa Fluor 488 fluorescent dye (ab150073-500, Abcam, Cambridge, MA, USA).
DOI: 10.15468/dl.qpsg8s
title: Occurrence Download

description: A dataset containing 51 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Laggera tomentosa (A.Rich.) Sch.Bip. ex Oliv. & Hiern"
 ]
}

 The dataset includes 51 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042025-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.usf3pm
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Justicia aquatica Benoist"
 ]
}

 The dataset includes 13 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042024-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bzdtsq
title: Occurrence Download

description: A dataset containing 592 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus pseudomacranthus Kitag."
 ]
}

 The dataset includes 592 records from 31 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042026-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.edvprn
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Laggera volkensii O.Hoffm."
 ]
}

 The dataset includes 5 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042027-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bgqjnz
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lathyrus lycicus Boiss. & Heldr."
 ]
}

 The dataset includes 4 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042028-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.w5pfyk
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Justicia arbuscula Benoist"
 ]
}

 The dataset includes 3 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042029-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4nkrzq
title: Occurrence Download

description: A dataset containing 19 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus pseudopanzerioides Krestovsk."
 ]
}

 The dataset includes 19 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042030-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vvm2kt
title: Occurrence Download

description: A dataset containing 34 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lagochilus acutilobus (Ledeb.) Fisch. & C.A.Mey."
 ]
}

 The dataset includes 34 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042031-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.x342rs
title: Occurrence Download

description: A dataset containing 101 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Justicia areysiana Deflers"
 ]
}

 The dataset includes 101 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042032-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rw4yp5
title: Occurrence Download

description: A dataset containing 647 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lathyrus marmoratus Boiss. & Balansa"
 ]
}

 The dataset includes 647 records from 31 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042033-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/7956069
title: ⏳ endoflife.date database (duckdb)
DOI: 10.15468/dl.btqmkb
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leonurus pubescens Benth."
 ]
}

 The dataset includes 15 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042034-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.77fv8v
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lagochilus alutaceus Bunge"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042035-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.82jexf
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Justicia argyrostachya (Nees) T.Anderson"
 ]
}

 The dataset includes 8 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042036-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8e5dn9
title: Occurrence Download

description: A dataset containing 589 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lathyrus miniatus M.Bieb. ex Steven"
 ]
}

 The dataset includes 589 records from 32 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0042038-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.