cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.23622679.v1
title: Use of immunohistochemistry and prion protein gene genotyping for detection of Scrapie in sheep in the state of Santa Catarina, Brazil

description: ABSTRACT: Scrapie is a contagious disease of sheep and goats caused by prions (PrPSc). This study described an outbreak of Scrapie in sheep in the state of Santa Catarina, Brazil. An 1-year and 3-month-old sheep developed clinical signs characterized by motor incoordination of the pelvic limbs, pruritus and alopecia for three days. The 38 sheep from the flock that were over 1 year of age underwent biopsies of the third eyelid and rectal mucosa, in addition to anti-PrPsc immunohistochemistry (IHC). Blood containing EDTA was collected for PRNP gene genotyping from these sheep. Of the 38, 16 (42.10%) had immunostaining againstPrPSc. IHC-positive animals were euthanized and necropsied, as well as lambs from positive mothers. Different organs of the 19 necropsied animals were collected in 10% buffered formalin for histopathological examination and anti-PrPSc IHC of the obex. The histopathology of the obex of the female with neurological signs presented discrete multifocal vacuolization of the cytoplasm of neurons and neuropil. The anti-PrPSc IHC showed that two out of the 19 obex samples had cytoplasmic immunostaining in neurons. The genotypes reported were ARQ/ARQ in 47.36%, ARR/ARQ in 36.84%, ARQ/VRQ in 10.52% and ARQ/VRR in 5.28%. The genotyping helps to identify susceptible animals and select animals more resistant to the development of Scrapie. The anti-PrPSc IHC from lymphoid biopsies, and genotyping demonstrated the high number of positive sheep classified in susceptible group.
DOI: 10.6084/m9.figshare.23622683
title: Does exergaming promote neurofunctional changes in Parkinson´s disease? A pilot clinical study

description: Abstract Introduction Previous studies have demonstrated beneficial effects in people with Parkinson´s disease trained with exergames. However, to the best of our knowledge, none of them evaluated whether these effects are sustained by neurofunctional changes. Objective To evaluate neurofunctional effects of a training, by means of functional magnetic resonance imaging, in people with Parkinson´s disease. Methods This study was a blind, randomized, and controlled pilot clinical trial with crossover design. The participants were submitted to an evaluation including cognitive performance and functional magnetic resonance imaging before and after the WiiTM or control trainings. Trainings were applied for 10 days, in two consecutive weeks. Participants starting with WiiTM training were then moved to the control training and vice versa. A wash-out period of 45 days between the trainings was respected. Results Memory, executive and visuo-spatial functions, and attention were significantly improved compared to baseline (p &lt; 0.05). No differences were observed in cognition compared to the control training. Though not significant, results of functional magnetic resonance imaging analyses suggested that WiiTM training could promote improvements on the brain functional connectivity especially in areas involved in motor execution, planning, visual, memory and somatosensory functions. Conclusion In people with Parkinson´s disease, an intensive WiiTM training improved cognitive performance that underlined neurofunctional changes in areas involved in cognitive processing.
DOI: 10.6084/m9.figshare.23622683.v1
title: Does exergaming promote neurofunctional changes in Parkinson´s disease? A pilot clinical study

description: Abstract Introduction Previous studies have demonstrated beneficial effects in people with Parkinson´s disease trained with exergames. However, to the best of our knowledge, none of them evaluated whether these effects are sustained by neurofunctional changes. Objective To evaluate neurofunctional effects of a training, by means of functional magnetic resonance imaging, in people with Parkinson´s disease. Methods This study was a blind, randomized, and controlled pilot clinical trial with crossover design. The participants were submitted to an evaluation including cognitive performance and functional magnetic resonance imaging before and after the WiiTM or control trainings. Trainings were applied for 10 days, in two consecutive weeks. Participants starting with WiiTM training were then moved to the control training and vice versa. A wash-out period of 45 days between the trainings was respected. Results Memory, executive and visuo-spatial functions, and attention were significantly improved compared to baseline (p &lt; 0.05). No differences were observed in cognition compared to the control training. Though not significant, results of functional magnetic resonance imaging analyses suggested that WiiTM training could promote improvements on the brain functional connectivity especially in areas involved in motor execution, planning, visual, memory and somatosensory functions. Conclusion In people with Parkinson´s disease, an intensive WiiTM training improved cognitive performance that underlined neurofunctional changes in areas involved in cognitive processing.
DOI: 10.6084/m9.figshare.23622685
title: Effects of indirect vocal therapy via telehealth in speech-language therapy on self-assessment and vocal quality of physical education professionals

description: ABSTRACT Purpose: this study aimed to verify the effects of indirect vocal therapy (IVT), via telehealth, in physical education professionals in voice self-assessment, as well as vocal quality. Methods: 16 physical education professionals, aged between 18 and 50 years, both sexes, participated in the study. All participants were submitted to eight IVT sessions twice a week, 30 minutes each, remotely. The assessments happened remotely, too. Vocal health was explained to modify vocal habits and general health, coping strategies, stress management, and therapeutic interaction. The following protocols were applied: Vocal Disorders Screening Index, Voice Symptoms Scale (VoiSS), Voice Handicap Index (VHI), and vocal recording (/a/ and counting) was performed, evaluating the general degree of vocal quality, pre and post-IVT. The Wilcoxon Test (p&lt;0.05) was applied to verify the findings. Results: there was a significant decrease in the values of the VoiSS protocol (total p=0.041; limitation p=0.032), and VHI (total p=0.012; p=0.005), and in the general degree of vocal quality in vowel /a/ (p=0.011). Conclusion: IVT applied through telehealth in physical education professionals, has positive effects on vocal and laryngopharyngeal symptoms, improving the self-perception of voice handicaps. IVT significantly improved the general degree of vocal quality, only in the emission of the sustained vowel.
DOI: 10.6084/m9.figshare.23622685.v1
title: Effects of indirect vocal therapy via telehealth in speech-language therapy on self-assessment and vocal quality of physical education professionals

description: ABSTRACT Purpose: this study aimed to verify the effects of indirect vocal therapy (IVT), via telehealth, in physical education professionals in voice self-assessment, as well as vocal quality. Methods: 16 physical education professionals, aged between 18 and 50 years, both sexes, participated in the study. All participants were submitted to eight IVT sessions twice a week, 30 minutes each, remotely. The assessments happened remotely, too. Vocal health was explained to modify vocal habits and general health, coping strategies, stress management, and therapeutic interaction. The following protocols were applied: Vocal Disorders Screening Index, Voice Symptoms Scale (VoiSS), Voice Handicap Index (VHI), and vocal recording (/a/ and counting) was performed, evaluating the general degree of vocal quality, pre and post-IVT. The Wilcoxon Test (p&lt;0.05) was applied to verify the findings. Results: there was a significant decrease in the values of the VoiSS protocol (total p=0.041; limitation p=0.032), and VHI (total p=0.012; p=0.005), and in the general degree of vocal quality in vowel /a/ (p=0.011). Conclusion: IVT applied through telehealth in physical education professionals, has positive effects on vocal and laryngopharyngeal symptoms, improving the self-perception of voice handicaps. IVT significantly improved the general degree of vocal quality, only in the emission of the sustained vowel.
DOI: 10.6084/m9.figshare.23622688
title: DGAT and LEP gene polymorphisms and their association with carcass characteristics and the lipid profile of meat from Nellore cattle

description: ABSTRACT The objective of this study was to evaluate the association of polymorphisms in the diacylglycerol acyltransferase ( DGAT ) and leptin ( LEP ) genes with the performance, carcass characteristics, meat quality, and lipid profile of Nellore cattle. A total of 100 intact male Nelore cattle were used to analyze the performance, carcass, physicochemical and centesimal composition, and fatty acid profile of beef. To identify the polymorphisms, the PCR–single-strand conformation polymorphism (SSCP) technique was applied to genomic DNA extracted from muscle tissue. The SSCP technique revealed the presence of four band patterns for the DGAT gene (AC, AD, AE and BB) with five alleles (A, B, C, D and E). For the LEP gene, five band patterns (AA, AB, AC, BB and BC) with three alleles (A, B and C) were observed. For the LEP gene, the AB genotype was associated with higher backfat thickness and ribs weight, while the BB genotype was associated with lower ribs yield; higher hindquarter yield was associated with AC and BB genotypes. Higher contents of C17:0, C18:0 and lower contents of C18:2ω6C, total polyunsaturated fatty acids, total ω6 and ratio of polyunsaturated and saturated fatty acids (PUFA/SFA) were verified for the AC genotype of the LEP gene. The AC and AA genotypes of the LEP gene were associated with higher means of C15:0 and C18:1ω9t. For the DGAT gene, the highest C24:0 content was associated with the AE genotype and the lowest with the AD and BB genotypes. Polymorphisms in the DGAT and LEP genes influence carcass parameters and the lipid profile of the meat of Nellore cattle.
DOI: 10.6084/m9.figshare.23622688.v1
title: DGAT and LEP gene polymorphisms and their association with carcass characteristics and the lipid profile of meat from Nellore cattle

description: ABSTRACT The objective of this study was to evaluate the association of polymorphisms in the diacylglycerol acyltransferase ( DGAT ) and leptin ( LEP ) genes with the performance, carcass characteristics, meat quality, and lipid profile of Nellore cattle. A total of 100 intact male Nelore cattle were used to analyze the performance, carcass, physicochemical and centesimal composition, and fatty acid profile of beef. To identify the polymorphisms, the PCR–single-strand conformation polymorphism (SSCP) technique was applied to genomic DNA extracted from muscle tissue. The SSCP technique revealed the presence of four band patterns for the DGAT gene (AC, AD, AE and BB) with five alleles (A, B, C, D and E). For the LEP gene, five band patterns (AA, AB, AC, BB and BC) with three alleles (A, B and C) were observed. For the LEP gene, the AB genotype was associated with higher backfat thickness and ribs weight, while the BB genotype was associated with lower ribs yield; higher hindquarter yield was associated with AC and BB genotypes. Higher contents of C17:0, C18:0 and lower contents of C18:2ω6C, total polyunsaturated fatty acids, total ω6 and ratio of polyunsaturated and saturated fatty acids (PUFA/SFA) were verified for the AC genotype of the LEP gene. The AC and AA genotypes of the LEP gene were associated with higher means of C15:0 and C18:1ω9t. For the DGAT gene, the highest C24:0 content was associated with the AE genotype and the lowest with the AD and BB genotypes. Polymorphisms in the DGAT and LEP genes influence carcass parameters and the lipid profile of the meat of Nellore cattle.
DOI: 10.6084/m9.figshare.23622689
title: Mechanisms of property rights protection in dairy agro-industrial systems: A study in Paraná, Brazil, and old Midi-Pyrénées, France

description: Abstract Purpose: This study aims to understand the mechanisms of property rights guarantee in the dairy agro-industrial system (DAGS) in Paraná, Brazil, and in the old Midi-Pyrénées region, France. Originality/value: From a complementary perspective of Transaction Cost Theory and Measurement Cost Theory, the proposal in the study is that the use of mechanisms to guarantee property rights through safeguards, parameters, measurement, agreements, or litigation contributes to the transaction of differentiated products and also to the protection of these rights, favoring systems improvement. Design/method/approach: A descriptive qualitative study was done, encompassing 25 semi-structured interviews with producers, processors, and key agents of the DAGS in the state of Paraná in Brazil and in the old Midi-Pyrénées region of France, in 2016 and 2017. Qualitative content analysis was performed with NVivo Pro software. Findings: It was identified that in France and in the central-eastern and western regions of Paraná, the use of complementary protection property rights mechanisms (safeguards, parameters, measurement, agreements, or litigation) allows higher quality milk transactions and reinvestments in production by agents, contributing to system improvement. In the northern region of Paraná, the limited use of property rights mechanisms (verbal agreement, measurement, unreliable information sharing, and the absence of ex post protection) allows minimal quality milk trans actions and restricts reinvestments in production, limiting systems improvement.
DOI: 10.6084/m9.figshare.23622689.v1
title: Mechanisms of property rights protection in dairy agro-industrial systems: A study in Paraná, Brazil, and old Midi-Pyrénées, France

description: Abstract Purpose: This study aims to understand the mechanisms of property rights guarantee in the dairy agro-industrial system (DAGS) in Paraná, Brazil, and in the old Midi-Pyrénées region, France. Originality/value: From a complementary perspective of Transaction Cost Theory and Measurement Cost Theory, the proposal in the study is that the use of mechanisms to guarantee property rights through safeguards, parameters, measurement, agreements, or litigation contributes to the transaction of differentiated products and also to the protection of these rights, favoring systems improvement. Design/method/approach: A descriptive qualitative study was done, encompassing 25 semi-structured interviews with producers, processors, and key agents of the DAGS in the state of Paraná in Brazil and in the old Midi-Pyrénées region of France, in 2016 and 2017. Qualitative content analysis was performed with NVivo Pro software. Findings: It was identified that in France and in the central-eastern and western regions of Paraná, the use of complementary protection property rights mechanisms (safeguards, parameters, measurement, agreements, or litigation) allows higher quality milk transactions and reinvestments in production by agents, contributing to system improvement. In the northern region of Paraná, the limited use of property rights mechanisms (verbal agreement, measurement, unreliable information sharing, and the absence of ex post protection) allows minimal quality milk trans actions and restricts reinvestments in production, limiting systems improvement.
DOI: 10.6084/m9.figshare.23622694
title: The influence of familial environment, parental perception and economic level on the receptive vocabulary of children

description: ABSTRACT Purpose: to examine the influence of familial environment, parental perception, and socioeconomic conditions on the receptive vocabulary of elementary school children. Methods: an analytical cross-sectional observational study. The students were selected by stratified proportional sampling, using the Receptive Vocabulary Test. Those responsible for the children answered the anamnesis and the inventory of family environmental resources. Initially, a bivariate analysis was performed using the chi-square test, considering p&lt;0.20. In the multivariate analysis of binary logistic regression, p&lt;0.05 was considered, and the quality of the model was evaluated through the Hosmer-Lemeshow method. Results: out of the 263 students, 131 were males, 142 were in the third grade and were on average 7.6 years old (±0.57), and 111 had a low receptive vocabulary. In the bivariate analysis, there was a relationship between parental perception about learning to read and write, school failure, and the outcome (p&lt;0.05). The variables environment, resources, and stability of family life were also used for multivariate analysis (p&lt;0.20). Conclusion: Parental perception of difficulty in reading and writing and a lack of family stability were factors associated with poor receptive vocabulary.
DOI: 10.6084/m9.figshare.23622694.v1
title: The influence of familial environment, parental perception and economic level on the receptive vocabulary of children

description: ABSTRACT Purpose: to examine the influence of familial environment, parental perception, and socioeconomic conditions on the receptive vocabulary of elementary school children. Methods: an analytical cross-sectional observational study. The students were selected by stratified proportional sampling, using the Receptive Vocabulary Test. Those responsible for the children answered the anamnesis and the inventory of family environmental resources. Initially, a bivariate analysis was performed using the chi-square test, considering p&lt;0.20. In the multivariate analysis of binary logistic regression, p&lt;0.05 was considered, and the quality of the model was evaluated through the Hosmer-Lemeshow method. Results: out of the 263 students, 131 were males, 142 were in the third grade and were on average 7.6 years old (±0.57), and 111 had a low receptive vocabulary. In the bivariate analysis, there was a relationship between parental perception about learning to read and write, school failure, and the outcome (p&lt;0.05). The variables environment, resources, and stability of family life were also used for multivariate analysis (p&lt;0.20). Conclusion: Parental perception of difficulty in reading and writing and a lack of family stability were factors associated with poor receptive vocabulary.
DOI: 10.6084/m9.figshare.22308810
title: Genome-wide association study revealed genetic loci for resistance to fusarium wilt in tomato germplasm

description: Abstract Tomato Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici (Fol) constrains tomato production worldwide. Three hundred forty tomato accessions were evaluated for Fusarium wilt resistance and single nucleotide polymorphisms (SNPs) associated with resistance. The disease resistance evaluation revealed that 15, 13, and 15 accessions were identified as Fusarium wilt resistant in Test 1, 2, and Mean data, respectively, with the disease severity index (DSI) ranging from 0-16.7%. A genome-wide association study (GWAS) identified SNPs associated with resistance. Eighteen common SNPs were detected in at least two tests and located on chromosomes 4, 6, 7, 9, and 12. Six unique significant SNPs were found in either Test 1 or 2, located on chromosomes 2, 4, and 7. Candidate genes associated with Fusarium wilt resistance were identified. Notably, two genes encoding leucine-rich repeat-like protein and disease-resistance protein were predicted from the two unique SNPs, solDsnp10606 and solDsnp6266, respectively.
DOI: 10.6084/m9.figshare.22308810.v1
title: Genome-wide association study revealed genetic loci for resistance to fusarium wilt in tomato germplasm

description: Abstract Tomato Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici (Fol) constrains tomato production worldwide. Three hundred forty tomato accessions were evaluated for Fusarium wilt resistance and single nucleotide polymorphisms (SNPs) associated with resistance. The disease resistance evaluation revealed that 15, 13, and 15 accessions were identified as Fusarium wilt resistant in Test 1, 2, and Mean data, respectively, with the disease severity index (DSI) ranging from 0-16.7%. A genome-wide association study (GWAS) identified SNPs associated with resistance. Eighteen common SNPs were detected in at least two tests and located on chromosomes 4, 6, 7, 9, and 12. Six unique significant SNPs were found in either Test 1 or 2, located on chromosomes 2, 4, and 7. Candidate genes associated with Fusarium wilt resistance were identified. Notably, two genes encoding leucine-rich repeat-like protein and disease-resistance protein were predicted from the two unique SNPs, solDsnp10606 and solDsnp6266, respectively.
DOI: 10.6084/m9.figshare.23622701.v1
title: The Definition and Typological Model of a Dogwhistle

description: Abstract A formal, speaker-based definition for the linguistic trope known as a ‘dogwhistle’ is provided. This definition is supported by an 11-part typological model for distinguishing dogwhistles from similar linguistic tropes (i.e., puns, innuendo, inside jokes) and other speech acts. The model is applied to many data examples from a variety of sources. The model allows for data input, filtering against the criteria, and classification of the speech act as a dogwhistle or not. Additionally, the model can highlight how well the data example adheres to certain criteria. This informs interpretations about whether the speech act is a successfully constructed dogwhistle as well as provide possible reasons for failed dogwhistles. This analysis deepens our understanding of political and social discourse and the ways it can be manipulated for personal gains, resulting in new insight that helps dismantle strategic racism (Haney-López, 2014) and other threats to democracy.
DOI: 10.6084/m9.figshare.23622701
title: The Definition and Typological Model of a Dogwhistle

description: Abstract A formal, speaker-based definition for the linguistic trope known as a ‘dogwhistle’ is provided. This definition is supported by an 11-part typological model for distinguishing dogwhistles from similar linguistic tropes (i.e., puns, innuendo, inside jokes) and other speech acts. The model is applied to many data examples from a variety of sources. The model allows for data input, filtering against the criteria, and classification of the speech act as a dogwhistle or not. Additionally, the model can highlight how well the data example adheres to certain criteria. This informs interpretations about whether the speech act is a successfully constructed dogwhistle as well as provide possible reasons for failed dogwhistles. This analysis deepens our understanding of political and social discourse and the ways it can be manipulated for personal gains, resulting in new insight that helps dismantle strategic racism (Haney-López, 2014) and other threats to democracy.