DOI: 10.5061/dryad.6vp96
title: Data from: A population genomic scan in Chorthippus grasshoppers unveils previously unknown phenotypic divergence

description: Understanding the genetics of speciation and the processes that drive it
 is a central goal of evolutionary biology. Grasshoppers of the Chorthippus
 species group differ strongly in calling song (and corresponding female
 preferences) but are exceedingly similar in other characteristics such as
 morphology. Here, we performed a population genomic scan on three
 Chorthippus species (Chorthippus biguttulus, C. mollis and C. brunneus) to
 gain insight into the genes and processes involved in divergence and
 speciation in this group. Using an RNA-seq approach, we examined
 functional variation between the species by calling SNPs for each of the
 three species pairs and using FST-based approaches to identify outliers.
 We found approximately 1% of SNPs in each comparison to be outliers.
 Between 37% and 40% of these outliers were nonsynonymous SNPs (as opposed
 to a global level of 17%) indicating that we recovered loci under
 selection. Among the outliers were several genes that may be involved in
 song production and hearing as well as genes involved in other traits such
 as food preferences and metabolism. Differences in food preferences
 between species were confirmed with a behavioural experiment. This
 indicates that multiple phenotypic differences implicating multiple
 evolutionary processes (sexual selection and natural selection) are
 present between the species.
description: TranscriptomeReference transcriptome for C. biguttulus as a fasta fileCbig_consRef.fastafinalfiltered.molbru.males.recodeVCF file containing filtered SNPs from the C. mollis / C. brunneus male comparisonfinalfiltered.molbru.females.recodeVCF file containing filtered SNPs from the C. mollis / C. brunneus female comparison.finalfiltered_brumol_maf10.recodeVCF file containing filtered SNPs from the C. mollis / C. brunneus comparison.finalfiltered.molbig.good.recodeVCF file containing filtered SNPs from the C. mollis / C. biguttulus comparison.finalfiltered.molbig.good.males.recodeVCF file containing filtered SNPs from the C. mollis / C. biguttulus male comparison.finalfiltered.molbig.good.females.recodeVCF file containing filtered SNPs from the C. mollis / C. biguttulus female comparisonfinalfiltered_bigbru_maf10.recodeVCF file containing filtered SNPs for the C. biguttulus / C. brunneus comparisonfinalfiltered.bigbru.males.recodeVCF file with filtered SNPs for the C. biguttulus / C. brunneus male comparisonfinalfiltered.bigbru.females.recodeVCF file with filtered SNPs from the C. biguttulus / C. brunneus female comparisonLositan files 2Compressed folder containing input files for LOSITAN. Bigbru - C. biguttulus / C. brunneus, Molbru - C. mollis / C. brunneus, Molbig - C. mollis / C. biguttulusBayescan input filesCompressed folder containing input files for BayeScan. Bigbru - C. biguttulus / C. brunneus, Molbru - C. mollis / C. brunneus, Molbig - C. mollis / C. biguttulusStructure FilesCompressed folder containing input files for Structure. Bigbru - C. biguttulus / C. brunneus, Molbru - C. mollis / C. brunneus, Molbig - C. mollis / C. biguttulusPopGen_grasshopper_IDsSpecies, sex, and population information for the IDs used in the VCF, Bayescan, Lositan, and Structure files.