DOI: 10.6084/m9.figshare.28891307.v1
title: <b>Limited thermal tolerance in tropical insects and its genomic signature</b>

description: Datasets from a study on thermal limits of terrestrial insects measured along two tropical elevation gradients. Includes physiological measurements of heat and cold tolerance, as well as comprehensive environmental data and predicted protein melting temperatures of insects.<b>Files and variables</b><b>File: Meltdata_FINAL.xlsx</b><b>Description:</b> All protein melting temperatures predicted with the deep learning model DeepSTABp from genomes of insect species downloaded from InsectBase 2.0<b>Variables</b>Protein: code for the proteinspcode: code for the speciesSpecies, Order, Family, Genus: taxonomic classificationGenome_size: total length of the assembled genome in base pairsN50_kb: length such that 50% of the total assembly length is contained in scaffolds/contigs equal to or longer than this lengthBUSCO_v4: Benchmarking Universal Single-Copy Orthologs, easure of genome completeness based on evolutionarily conserved genesSource: source of genomeAnnotation: protein information<br><b>File: Ecostress_Kenya.xlsx</b><b>Description:</b> Surface temperatures for all study plots in East Africa were derived from the ECOSTRESS sensor, a radiometer mounted on the International Space Station.<b>Variables</b>PointID (surface reference point)PlotID (study plot name)LatitudeLongitudeDateTemperature (in °C)Elevation (in meters above sea level)<br><b>File: Ecostress_Peru.xlsx</b><b>Description:</b> Surface temperatures for all study plots in the Neotropics were derived from the ECOSTRESS sensor<b>Variables</b>PointID (surface reference point)PlotID (study plot name)LatitudeLongitudeDateTemperature (in °C)Elevation (in meters above sea level)<br><b>File: ThermalLimits_Barcoded.xlsx</b><b>Description:</b> All physiological, environmental and taxonomic data for thermal limit analyses of barcoded insects<b>Variables</b>Sampleid (individual insect ID)Plot (name of collection site)Season (sampling period)Light (sampling condition)Weather (sampling condition)CTmin (critical thermal minimum)CTmax (critical thermal maximum)Regain_mov (check if insect regained movement within 1 h after experiment)ObserverExperimentIncrease_rate (time after which temperature was increased by 1°C in min)Shock (indicates heat/cold shock before experiment)TMS (Air temperature in Peru from temperature loggers, in Kenya from CHELSA satellite climate)LatitudeLongitudeElevation (in meters above sea level)bio1 (mean annual temperature from CHELSA)bio5 (maximum temperature of warmest month from CHELSA)bio6 (maximum temperature of coldest month from CHELSA)Processid (barcode process ID)Current.ID (preliminary field identification)Current.Order (preliminary field identification)Match Overlap.(bp) (taxonomic identification from BOLD)Match.Phylum (taxonomic identification from BOLD)Match.Class (taxonomic identification from BOLD)Match.Order (taxonomic identification from BOLD)Match.Family (taxonomic identification from BOLD)NoteMatch.Genus (taxonomic identification from BOLD)Match.Species (taxonomic identification from BOLD)Match.Subspecies (taxonomic identification from BOLD)Match.Processid (matching barcoding ID from BOLD)Match.BIN (matching BIN from BOLD)OTUNr (operational taxonomic unit from sequence clustering)<b>File: ThermalLimits_All.xlsx</b><b>Description:</b> All physiological, environmental and taxonomic data for thermal limit analyses of all collected insects (including samples without genetic data)<b>Variables</b>Sample_ID (individual insect ID)Plot (name of collection site)Season (sampling period)Order (field identification)Light (sampling condition)Weather (sampling condition)CTmin (critical thermal minimum)CTmax (critical thermal maximum)Regain_mov (check if insect regained movement within 1 h after experiment)ObserverExperimentIncrease_rate (time after which temperature was increased by 1°C in min)Shock (indicates heat/cold shock before experiment)TMS (Air temperature in Peru from temperature loggers, in Kenya from CHELSA satellite climate)LatitudeLongitudeElevation (in meters above sea level)bio1 (mean annual temperature from CHELSA)bio5 (maximum temperature of warmest month from CHELSA)bio6 (maximum temperature of coldest month from CHELSA)<b>File: Analysis.r</b><b>Description:</b> R code to analyse and create plots included in the publicationPackage installation usually &lt;3 min<br><b>Code/software</b>Excel v. 2503R v.4.5.0 (min 4.4.3)<br>