DOI: 10.5061/dryad.f7m0cfz6w
title: EEG Recordings for: Optogenetic stimulation of the dorsal striatum bidirectionally controls seizures

description: Engagement of the basal ganglia experimental seizures was first observed
 almost 75 years ago. However, the role of the basal ganglia’s input
 nucleus, the striatum, in seizure control is relatively understudied. To
 address this gap, we used an optogenetic approach to activate and
 inactivate neurons in the dorsal striatum of rats submitted to the
 gamma-butyrolactone (GBL) model of absence epilepsy, amygdala kindling
 model of temporal lobe epilepsy, and pilocarpine-induced Status
 Epilepticus (SE).  Open-loop (continuous light delivery)
 optogenetic activation of  dorsal striatal neurons robustly
 suppressed seizures in all models. By contrast, open-loop optogenetic
 silencing increased absence seizure expression and facilitated SE onset
 but had no effect on kindled seizures. In the GBL model, we also tested
 the effects of closed-loop modulation (light delivery in response to
 seizure detection). Closed-loop activation reduced duration of spike-wave
 discharges (SWDs), while closed-loop inhibition increased SWD duration.
 This dataset includes electroencephalographic recordings for each subject
 and test session in the manuscript are included as european data format
 (.edf) files.  These results demonstrated previously unrecognized
 anti-absence effects associated with striatal neuromodulation. These
 findings demonstrate a robust, bidirectional role of the dorsal striatum
 in the control of multiple seizure types, suggesting that the striatum is
 a site that can exert broad-spectrum control of seizures. 
description: # EEG Recordings for "Optogenetic stimulation of the dorsal striatum
 bidirectionally controls seizures" Dataset DOI:
 [10.5061/dryad.f7m0cfz6w](10.5061/dryad.f7m0cfz6w) ## Description of the
 data and file structure ### Files and variables #### File: Kindling.zip
 **Description:** A zip archive with folders containing European Data
 Format (EDF) electroencephalography (EEG) files for each test session for
 each subject, organized by group, stimulation frequency, and wavelength of
 light. Files show a single kindling session for each animal with
 electrographic activity recorded from a depth electrode implanted into the
 basolateral amygdala and stimulator feedback (when relevant) showing the
 timing of light delivery. Data are organized into folders. *
 "Kindling - GFP" are recordings from animals injected with a
 control AAV virus expressing green flourescent protein but no opsin. *
 "Kindling - ChR2" are recordings from animals injected with AAV
 coding for channelrhodopsin-2 (ChR2) to activate the striatum. *
 "Kindling - ArchT" are recordings from animals injected with AAV
 coding for archaerhodopsin (ArchT) to inhibit the striatum. * Subfolders
 within each of these folders indicate the light stimulation delivered to
 the striatum ("No light" - no light was delivered; "Blue
 Light" indicating 450 nm light was delivered into striatum;
 "Green Light" indicating that 532 nm light was delivered into
 the striatum. * Each file is named following the convention ANIMAL
 IDENTIFIER-VIRUS-Session.  #### File: GBL.zip **Description:** A zip
 archive with folders containing European Data Format (EDF)
 electroencephalography (EEG) files for each test session for each subject,
 organized by group, stimulation frequency, and wavelength of light. Files
 show a single session for each animal with electrographic activity
 recorded from surface electrodes and stimulator feedback (when relevant)
 showing the timing of light delivery.  Data are organized into folders.  *
 Closed Loop - GBL * "ArchT" - folder containing recordings from
 animals injected with AAV expressing ArchT to inhibit the striatum.  *
 "ChR2" - folder containing recordings from animals injected with
 AAV expressing ChR2 to activate the striatum.  * "5 Hz" -
 subfolder containing recordings from sessions with 5 Hz optogenetic
 stimulation of striatum * "100 Hz"  - subfolder containing
 recordings from sessions with 100 Hz optogenetic stimulation of striatum *
 "GFP" - folder containing recordings from animals injected with
 AAV expressing GFP as a control.   * "Blue 5 Hz" - subfolder
 containing recordings from sessions with 5 Hz light delivery (450nm
 wavelength light) * "Blue 100 Hz" - subfolder containing
 recordings from sessions with 100 Hz light delivery (450nm wavelength
 light) * "Green 100 Hz" - subfolder containing recordings from
 sessions with 100 Hz light delivery (532nm wavelength light) * Open Loop -
 GBL * "GBL - ArchT" - folder containing recordings from animals
 injected with AAV expressing ArchT to inhibit the striatum.  * "Green
 Light" subfolder containing recordings from sessions with 100 Hz
 light delivery (532nm wavelength light) to inhibit the striatum. *
 "No Light" - subfolder containing recordings from sessions with
 no light delivery as a control * "GBL - ChR2" - folder
 containing recordings from animals injected with AAV expressing ChR2 to
 activate the striatum.  * "5 Hz" - subfolder containing
 recordings from sessions with 5 Hz optogenetic stimulation of striatum *
 "100 Hz"  - subfolder containing recordings from sessions with
 100 Hz optogenetic stimulation of striatum * "No
 Light" - subfolder containing recordings from sessions with no light
 delivery as a control * "GBL - FP" - folder containing
 recordings from animals injected with AAV expressing GFP as a control.   *
 Blue Light - subfolder containing subfolders for different test sessions *
 "Blue 5 Hz" - subfolder containing recordings from sessions with
 5 Hz light delivery (450nm wavelength light) * "Blue 100 Hz"
 - subfolder containing recordings from sessions with 100 Hz light delivery
 (450nm wavelength light) * "Green 100 Hz" - subfolder containing
 recordings from sessions with 100 Hz light delivery (532nm wavelength
 light) * "No Light" - subfolder containing recordings from
 sessions with no light delivery as a control #### File: Pilocarpine.zip
 **Description:** A zip archive with folders containing European Data
 Format (EDF) electroencephalography (EEG) files for each test session for
 each subject, organized by group, stimulation frequency, and wavelength of
 light. Files show the complete session from pilocarpine administration
 through 90 minutes post treatment, for each animal with electrographic
 activity recorded from surface electrodes and stimulator feedback (when
 relevant) showing the timing of light delivery.    Data are organized into
 folders: * "Pilocarpine - GFP" are recordings from animals
 injected with a control AAV virus expressing green flourescent protein but
 no opsin. * "Pilocarpine - ChR2" are recordings from animals
 injected with AAV coding for channelrhodopsin-2 (ChR2) to activate the
 striatum. * "Pilocarpine - ArchT" are recordings from animals
 injected with AAV coding for archaerhodopsin (ArchT) to inhibit the
 striatum. * Files are named according to the convention ANIMAL
 ID-VIRUS-TYPE OF LIGHT DELIVERED. #### File: Quantification.xlsx
 **Description:** An excel file showing the values obtained following
 quantification of seizures for each animal present in the parent study.
 The file has four sheets:  * GBL-open loop: Quantification of
 spike-and-wave discharges during open loop neuromodulation (and paired
 control trials) for all animals included in the study * GBL-closed loop:
 Quantification of spike-and-wave discharges during closed loop
 neuromodulation (with within-session control (non stimulated)
 spike-and-wave discharges) for all animals included in the study. *
 kindling: Quantification of electrographic seizure activity
 (Afterdischarge [AD] duration and behavioral seizure score) for kindling
 experiments for all animals in the study.  * pilocarpine: Quantification 
 of latency to status epilepticus (SE) for pilocarpine experiments for all
 animals in the study.  For each of the above, animal data is organized in
 rows with subject identifiers (e.g., M3L) followed by data for the range
 of conditions the animal was tested under for each model.  ## Usage Notes
 Several options are available for viewing EDF files:  *
 https://github.com/kostasrotas/EDFViewer-Desktop * A stand alone EDF
 Viewer available for Windows, MacOS, and Ubuntu *
 https://github.com/holgern/pyedflib * A python-based EDF Viewer *
 https://www.mathworks.com/help/signal/ref/edfread.html * Matlab natively
 supports EDF viewing through the edfread command ## Code/software N/A ##
 Access information Other publicly accessible locations of the data: * N/A
 Data was derived from the following sources: * N/A