cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.65
title: HBS1L-MYB INTERGENIC REGION EXPRESSION IS ASSOCIATED WITH FETAL HEMOGLOBIN LEVEL IN β-THALASSEMIA/HbE ERYTHROBLASTS

description: β-thalassemia/HbE is a hematological disease caused by impairment of β-globin production. The degree of diseases severity depends on several factors including the expression of fetal globin (α2γ2) which can compensate for an accumulation of excess α-globin chains. GWAS analysis were performed to identify loci that are linked to reduction in disease severity. A subset of key loci is located at or near the genes encoding transcription factors functionally associated with globin gene expression. The polymorphisms at the intergenic region between HBS1L and MYB gene or HMIR was shown to be one of the significant severity modifying factor. A set of short transcripts corresponding to the HBS1L-MYB genes intergenic region was found in RNA-seq analyses of K562 erythroid cell line, indicating that this intergenic region is actively transcribed. Recently, the non-coding RNA was shown to play roles in gene transcription control. Quantitative PCR was carried out to determine and quantify the non-coding HBS1L-MYB transcripts in primary human erythroblasts derived from normal individuals and β-thalassemia/HbE patients. Three clusters of HMIR transcripts were identified. Interestingly, erythroblasts from β-thalassemia/HbE patients have significantly lower levels of the HMIR transcripts than those of normal erythroblasts. In order to characterize these HMIR transcripts, the 5ʹ and 3ʹ rapid amplification of cDNA ends (RACE) were carried out to detect the full length of the transcripts. Amplifying results of RACE products show that the 5ʹend of each transcripts are more than 200 base pairs. The clones were selected for DNA sequencing, however, they do not contain HMIR sequences. Hence, these transcripts are the candidate for being a small non-coding RNAs that might responsible for modifying the patient’s severity.
DOI: 10.14457/cu.the.2015.66
title: THE STUDY OF SEAT PRESSURE DISTRIBUTION PATTERN IN OFFICE WORKER WITH AND WITHOUT CHRONIC LOW BACK PAIN

description: This study aimed to investigate seat pressure distribution characteristics, i.e. average pressure (AP), peak pressure ratio (PP ratio), frequency of postural shift, and body perceived discomfort (BPD) during 1-hour sitting in office workers with and without chronic low back pain (LBP). Forty-six participants (chronic LBP = 23 and control = 23) typed a standardized text passage at a computer work station for an hour. A seat pressure mat device was used to collect the seat pressure distribution data. The results showed that workers with chronic LBP sat significantly more asymmetrically than their healthy counterparts. During 1-hour sitting, all workers assumed slumped sitting postures after 20 minutes of sitting. Healthy workers had significantly more frequent postural shifts than chronic LBP workers during prolonged sitting. The findings suggest that a person should avoid an asymmetrical sitting posture, change postures frequently, and have a short break from sitting every 20 minutes.
DOI: 10.14457/cu.the.2015.67
title: The preventive effect of isoferulic acid on monosaccharides and methylglyoxal-mediated glycation

description: Protein glycation is a non-enzymatic modification of protein by reducing sugars (such as glucose and fructose) or dicarbonyl molecules (such as methylglyoxal), resulting in the formation of advanced glycation end products (AGEs) which have been considered to be a significant contributor of age-related diseases and diabetic complications. Isoferulic acid (IFA), one of cinnamic acid derivatives in plants and rhizome of Cimicifuga species, has been shown various pharmacological activities including anti-oxidation and anti-hyperglycemia. The objectives of the present study were to investigate anti-glycation property of IFA in different models including bovine serum albumin (BSA), deoxyribonucleic acid (DNA) and pancreatic β cell. The results showed that IFA (1.25-5 mM) inhibited formation of fluorescent and non-fluorescent AGE, Amadori product (fructosamine), protein carbonyl content, loss of protein thiol groups, and amyloid cross β structure in BSA glycation induced by glucose, fructose and methylglyoxal. IFA (0.1-1 mM) prevented oxidative DNA strand breakage and suppressed superoxide anion and hydroxyl radical production induced by MG and lysine, however, the result from HPLC indicated that IFA did not directly trap MG. In pancreatic β cell, IFA (0.1 mM) prevented β-cell dysfunction by reducing intracellular reactive oxygen species (ROS) and uncoupling protein 2 (UCP2) expression, and improving insulin secretion. Although IFA could not elevate ATP level, it increased glyoxalase I activity in MG-detoxification system and β-cell viability, and also inhibited caspase-3 activity in β cell induced by MG. From these findings, IFA might be considered or applied to use in prevention of AGE-mediated diabetic complications.
DOI: 10.14457/cu.the.2015.68
title: Evaluation efficacy of dried blood spot filter paper for HIV-1 viral load and Drug resistance genotyping detection

description: Currently, although a number of HIV-1 infected people increases continuously, the number of death people caused by HIV-1 disease reduces as compared to the death people in the past. Because patient is monitored continually by detecting the HIV-1 viral load and HIV-1 drug resistance, which is used to guide a HIV-1 therapy. In addition, a dried blood spot (DBS), which is used for collecting sample, is used for detecting the viral load and drug resistance genotyping because it is convenience to be collected and transported to both urban and rural area. Whatman 903 (W-903) filter paper has been used to detect HIV-1 viral load and HIV-1 drug resistance. However, the cost of Whatman 903 filter paper is still expensive. The objective of this research was to evaluate the performance of two new dried blood spot filter papers, nitrocellulose membrane and cellulose fiber sample pad, which cost is cheaper than Whatman 903 filter paper for collecting the dried blood spot in order to be used to detect HIV-1 load and HIV-1 drug resistance. The dried blood spot specimens were collected from 70 blood samples which HIV-1 viral load and HIV-1 drug resistance were measured by NucliSENS EasyQ HIV-1 v2.0 and ViroSeq HIV-1 Genotyping system, respectively. Moreover, we also evaluated an ability of gold nanoparticles and anion polymer-coated magnetic beads in order to increase the sensitivity of HIV-1 viral load detection of filter papers. The experimental results showed that nitrocellulose membrane was not capable of absorbing the dried blood spot therefore only Whatman 903 and cellulose fiber sample pad performance were evaluated. On the other hand, when used anion polymer-coated magnetic beads with filter papers, the HIV-1 viral load could not be interpreted. This is because the anion polymer-coated magnetic beads interfere the reaction in HIV-1 viral load detection. Although gold nanoparticles did not interfere the HIV-1 viral load detection reaction, the detected HIV-1 viral load from Whatman 903 with/without gold nanoparticles and cellulose fiber sample pad with/without gold nanoparticles were related. This means that gold nanoparticles did not affect an increasing in an ability of HIV-1 viral load detection. Since cellulose fiber sample pad could be used to detect the HIV-1 viral load similarly to Whatman 903, we therefore evaluated the performance of Whatman 903 and cellulose fiber sample pad to detect HIV-1 drug resistance from dried blood spot. The experimental results showed that these filters detected the amino sequencing which was the cause of virus mutation. Therefore, the performance of cellulose fiber sample pad should be investigated comprehensively in order to be used in practical.
DOI: 10.14457/cu.the.2015.69
title: Effects of herbal plant extracts on oxidative stress and neuroinflammatory mechanisms in cultured neuronal cells : a model for alzheimer's disease prevention

description: Traditional medicine employs a wide range of native herbs containing a variety of substances which are used to treat several disorders. Amaranthus plants or spinach are rich in antioxidant compounds which play a role in scavenging free radicals and are known to possess medicinal properties. Oxidative stress caused by aberrant production of reactive oxygen species (ROS) and neuroinflammation represents important mechanisms for neuronal dysfunction and cell loss of many neurodegenerative diseases such as Alzheimer's disease (AD). Advanced glycation endproducts (AGEs) can lead to the pathological changes of AD by interaction with their receptor (RAGE) elicits the formation of ROS that are believed to occur early in AD pathology. So far no work has been reported on neuroprotective effect of Amaranthus extract on AGEs-induced AD pathology. In the present study, petroleum ether, dichloromethane and methanol were used to extract leaves of Amaranthus lividus and Amaranthus tricolor; the extracts were analyzed for antioxidant activity which was found to be the highest in the methanol fraction of both kinds of the plants. Human neuroblastoma cell lines, SH-SY5Y, were induced to oxidative damage upon incubation with AGEs as shown by an increase in oxidative stress as well as a significantly upregulated oxidative gene, HMOX-1 resulting in reducing cell viability and increasing cell toxicity in a dose dependent manner. AGEs could induce RAGE and the consequent activation of NF-κB genes expression and were able to upregulate BACE1, PS1 expression that involved in amyloid beta production, pathologic hallmark of AD, and gene expression of pro-inflammatory cytokines, TNF-α, IL-1 and IL-6. Upon incubation with A. lividus and A. tricolor extracts, they were effective at reducing oxidative stress and were dose dependently capable to attenuate the neuron toxicity caused by AGEs treatment. Interestingly, the extracts significantly decreased the expression of the HMOX-1, RAGE and NF-κB genes. Moreover, the results showed BACE1, PS1 and proinflammatory cytokine genes expression were significantly downregulated when AGEs-induced cells were treated with the plant extracts. The present data suggest that A. lividus and A. tricolor extracts not only have neuroprotective effect against AGEs-induced oxidative damage but also have anti-inflammatory activity by reducing pro-inflammatory cytokine gene expression and attenuate Alzheimer-like pathophysiological changes by down-regulating the key enzyme for amyloid beta production. The neuroprotective effects of these plants may be associated with their inhibitory actions via the RAGE/NF-kB pathway. The present data support the utilization of these plants for beneficial effect on the cognitive performance from AD and may provide a new to the possibility of using the herbal extracts for potential therapy of AD.
DOI: 10.14457/cu.the.2015.70
title: PLEANG : WINDOW DESIGN METHODOLOGY FOR PRINCE NARIS LEARNING CENTER

description: H.R.H. Prince Narisaranuvativongse (1863-1947) worked in various art fields, including architecture during the reign of King Chulalongkorn (King Rama V, r. 1868- 1910) until the reign of King Prajadhipok (King Rama VII, r. 1925-1935). Under various design constraints, such as political issues and socio-acceptance, he accomplished the mission with remarkable solutions. Although, Prince Naris was an autodidact in the art field, he collaborated with numerous Italian artists, sculptures, engineers and architects who came to work for the government of Thailand; enabling him to think unconventionally. Prince Naris is not a traditionalist. The thesis is to study of Prince Naris’ window design methodology, so called by him in Thai as Pleang (แผลง). The thesis is especially about the reconfiguration of prefabricated windows used at his house/studio, at Baan Plainern. The objective of this thesis is to reconfigure the windows from the study, and to create connection between Baan Plainern and general public through architecture. In addition, Baan Plainern is where Prince Naris had lived for the last phase of his life. Also, the Naris Foundation is a charitable organisation that support Thai arts and supplement Prince Naris’ artifacts. The remarkable of the Naris Foundation can be along with the statement of architecture which represent it. The proposition of the design is the selection of materials that gives contrast between the old and the new, heaviness and lightness, darkness and brightness. The final result reflects the study of Prince Naris’ window design methodology through the building as it catches light and cast shadow in the space.
DOI: 10.14457/cu.the.2015.71
title: STRENGTHS AND LIMITATIONS ANALYSIS OF SECOND-HANDED REAL ESTATE DATABASE SYSTEM UNDER THAI REAL ESTATE BROKER ASSOCIATION (TREBA)

description: This research aims to study and analyze strengths and limitations of a second-handed real estate database system under Thai Real Estate Broker Association (TREBA) including input data, output data and its source of data by comparing it with the standard dataset from Real Estate Transaction Standard (RETS) under National Associations of REALTORS® (NAR). The data collection is from the existing database systems for the real estate brokers in 10 ASEAN countries and top 6 world’s economic influencers by attending special seminars, TREBA’s monthly meetings and an annual conference together with the in-depth interview with the TREBA executive committees, TREBA members and the system developer and, in addition, with the expert interview with local linguistics experts. The study found that (1) limitations that cause the MLS trial system under TREBA to be inactive are a lack of knowledge and understanding about the concept and working process of the MLS. However, the potential improvement is to allow TREBA starting realizing the advantages of the use of MLS including the affiliating with NAR to coordinate with the other countries that plan to implement this concept (2) The standard dataset of 10 groups with 889 words set by RETS is applicable with all 16 sampling countries, but the difference of the language, perception, law and the economic, social and cultural background has an impact on the terminology definition. (3) To define the terminology should co-operate with the experts from linguistics, architecture, law and the related fields in order to apply it in accordance with the correct meaning. This study illustrates the limitations of the MLS improvement under TREBA to be linked with the other countries, typically the terminology and its definition. Accordingly, the correct knowledge and understanding of the MLS system. If TREBA is a pioneer to launch this project, it should promote the correct understanding and knowledge of the MLS concept, clarify the user requirement to the system designer, set up the standard of the data restriction, and take the action to improve the MLS under TREBA
DOI: 10.14457/cu.the.2015.72
title: Adaptive Reuse of Old Shophouses in Bangkok

description: Currently in central Bangkok where land value is high, the demand for space in various forms has been increasing steadily. This is a result of the urban development that has grown rapidly over the last 10 years. Today, there are many shophouses in Bangkok that have been in vacant condition or underutilized. The demolition of particular slot and reconstruction in the same footprint as original building is impossible because of the setback requirement. Therefore, the reuse of old shophouse is necessary. Thus, this research aims to provide guidelines for reusing the old shophouse to support the changes in physical components, spatial organization and adaptability. The research methods include 1) reviewing the academic documents related to the shophouse and theory related to adaptability and 2) surveying spatial and physical characteristic of the shophouse in Siam Square area. Photographic documentation and hand sketch are the primary method of collecting information in order to analyze the usage patterns. The findings reveal that the spatial characteristic of shophouse shows irregularity and obscurity of the usage patterns which depends on the type of businesses. While, the physical characteristic has high rigidity in structural layout, dimension and supporting utility. This physical characteristic has already determined the circulation, location of sewage system and floor slab that are difficult to modify to follow the usage patterns. This contrary is the key reason that make shophouse difficult to adapt. Therefore, the analysis from the survey has led to the proposal of design guideline for reusing old shophouse in Bangkok. In order to accommodate changes in the physical and spatial configuration in the future, the supporting utility should be placed along with the structural columns that is distributed throughout. The stair and slab should be designed as a modular unit that can be filled in or moved out easily. The façade should be designed as a frame with adaptable module that corresponds to the interior space. This design guideline is implemented in the vacant shophouse in Asoke to study the possibility, as well as advantages and disadvantages of the design proposal. Finally, it is anticipated that the knowledge gained from this study will provide architects, building owners, and users of old shophouse with appropriate adaptive reuse design alternatives.
DOI: 10.14457/cu.the.2015.73
title: ECO-INDUSTRIAL ESTATE DEVELOPMENT IN THAILAND: A CASE STUDY OF AMATA NAKORN INDUSTRIAL ESTATE

description: Greening industrial activities is one of a key success for sustainable development. This research aims to investigate the status of eco-industrial estate development in Thailand and to identify the significant factors affecting eco-industrial development using Amata Nakorn Industrial Estate as a case study. There have been several attempts between Thai and Japanese government to transform existing Thai industrial estates into eco-industrial estate since there is a significant proportion of Japanese factories in Amata Nakorn industrial estate and in Thailand. However, previous attempts have stranded or with very little progress. There have been not studies to investigate the key factors impacting the transformation. Several factors were identified during the One Stop Service (OSS) project implementation by using questionnaire survey. The questionnaires were distributed to all tenant companies in Amata Nakorn Industrial Estate to identify key factors affecting their decision to participate in eco-industrial project. Key factors were identified for improving potential implementation of OSS project. The research result found out that the government’s active involvement is needed for the development. The most important expectation of tenants from OSS project is a cost reduction on waste management and most important factor affecting making decision on using OSS is a reasonable service fee. A foreign company involvement has no significant impact on decision making process. The tenant factories preferred more involvement of the government in the development of the project. In summary, Strategies to promote future eco-industrial estate development in Thailand and Amata Nakorn were suggested based on key factors and SWOTs analysis and the actual project observation experiences.
DOI: 10.14457/cu.the.2000.35
title: การประยุกต์เทคนิคคิวเอฟดีเพื่อปรับปรุงคุณภาพของงานบริการในฝ่ายขาย ของบริษัทจำหน่ายรถบรรทุก

description: วัตถุประสงค์ของการวิจัยนี้ เพื่อปรับปรุงคุณภาพของงานบริการของฝ่ายขายบริษัทกรณีศึกษาให้สนองต่อความต้องการและความพึงพอใจของลูกค้าทั้งภายในและภายนอก ซึ่งในปัจจัยด้านคุณภาพของงานบริการระหว่างการขาย สามารถปรับปรุงได้ในส่วนขายตรง ฝ่ายขายเอง เทคนิคหลักที่ใช้ในการวิจัยได้แก่เทคนิคคิวเอฟดี หรือเทคนิคการแปรหน้าที่คุณภาพ ( Quality Function Deployment ) โดยเทคนิคนี้แบ่งออกเป็น 4 ช่วงได้แก่ (1) การวางแผนผลิตภัณฑ์หรือบ้านแห่งคุณภาพ ( Product Planning or House of Quality ) เป็นขั้นตอนในการเก็บข้อมูลความต้องการของลูกค้าที่เป็นคำพูด แปรออกมาเป็นข้อกำหนดทางเทคนิคที่ใช้กันในบริษัท (2) การออกแบบผลิตภัณฑ์ ( Product Design ) เป็นขั้นตอนที่แปรจากข้อกำหนดทางเทคนิค ออกมาให้ เป็นคุณสมบัติต่าง ๆ ที่ต้องการ (3) การวางแผนกระบวนการ ( Process Planning ) เป็นการวิเคราะห์และวางแผนเลือกกระบวนการที่จะใช้ปรับปรุง โดยพิจารณาว่ากระบวนการดังกล่าวต้องสามารถตอบสนองคุณสมบัติได้ (4) การวางแผนขั้นตอนการผลิต และควบคุมกระบวนการ ( Production Operations Planning and Process Control ) เป็นการนำเอากระบวนการในช่วงที่ 3 ไปกำหนดวิธีการควบคุม วิธีการตรวจสอบ ผู้รับผิดชอบในการดำเนินงาน ผลลัพธ์ที่ได้จากเทคนิคคิวเอฟดีที่นำมาใช้ในการปรับปรุงคุณภาพของงานบริการ ประกอบด้วย 4 กระบวนการหลัก โดยเรียงตามลำดับความสำคัญคือ(1) การบริการอย่างเป็นมิตรกับลูกค้า ( Customer Friendly Service ) เป็นกระบวนการที่เน้นการเอาใจใส่ ติดตามดูแลลูกค้าอย่างใกล้ชิด (2) การควบคุมข้อมูลลูกค้า ( Customer Database Control ) เป็นการนำเทคโนโลยีสารสนเทศในการจัดหมวดหมู่รวบรวมข้อมูลและวิเคราะห์ลูกค้า(3) การวิเคราะห์คุณภาพงานบริการ( SQS )และการตรวจติดตามคุณภาพงานบริการ( SQA ) เป็นการจัดให้มีทั้งมาตราฐานในการปฏิบัติงานและตรวจสอบงานในทุกจุดบริการ(4) การบริการสนับสนุนบุคลากร ( Employee Support Service) เป็นการสร้างความพึงพอใจให้แก่บุคลากร ซึ่งก็คือลูกค้าภายในของบริษัทเพื่อขยายผลไปยังกลุ่มลูกค้าภายนอก ซึ่งเป็นกลุ่มลูกค้าหลักของบริษัทต่อไป จากการปรับปรุงดังกล่าวสามารถทำให้ติดต่อกับลูกค้าได้เร็วขึ้น, ได้รับข่าวสารข้อมูลจากลูกค้าละเอียดมากขึ้น , ลดเวลาในการรอการบริการลง , สร้างภาพลักษณ์ใหม่ในงานบริการ , มีระบบการพัฒนางานบริการอย่างต่อเนื่อง , มีการทำงานที่เป็นมาตราฐานและตรวจสอบได้ ตลอดจนสร้างบรรยากาศที่ดีระหว่างลูกค้าและพนักงานขาย หลังจากที่ได้ทดลองนำไปปฏิบัติจริงเป็นเวลา 2 เดือนที่ส่วนขายตรง ฝ่ายขาย แล้วทำการวัดผลโดยเทคนิคกระบวนการลำดับขั้นเชิงวิเคราะห์( AHP ) พบว่าลูกค้ามีความพึงพอใจต่อระบบงานบริการใหม่มากขึ้น
DOI: 10.14457/cu.the.2000.36
title: Cosmic ray transport and acceleration near an oblique, spherical shock

description: A suitable Fokker-Planck transport equation has been developed to describe the behavior of charged particles for an Archimedean spiral magnetic configuration and spherically symmetric fluid flow. Simulations of the pitch angle transport and acceleration of cosmic rays nearby an oblique, spherical shock have been performed. As part of this study, we have developed a generalized total variation diminishing (TVD) method based on one of the well-known numerical techniques to handle the advection problems. By using our developed spatial transport technique, the program running speed can be improved to be 1-2 orders of magnitude faster than a prototype that was developed by Ruffolo (1995), with an acceptable numerical error. The spatial step size can be fully adjusted to a desired general, Courant number (γ = u∆t/∆z), which is restricted not to exceed unity in the standard TVD scheme.
DOI: 10.14457/cu.the.2000.37
title: Folate status in healty women, pregnancy and women taking oral contraceptives in Health Promotion Center region 4 Ratchaburi

description: Folate, a B vitamin is necessary for metabolic function in human body. This study determined folate content in serum and red blood cell of healthy women, non-pregnancy taking oral contraceptives, pregnancy who have never taken oral contraceptives before pregnant and pregnancy using oral contraceptives before pregnant in HEALTH PROMOTION CENTER REGION 4 by microbiological method using Lactobacillus casei, ATCC. No.7469. The folate levels in serum and red blood cell of 38 healthy women were 12.29 ± 5.91 and 123.22 ± 46.04 ng/ml., respectively. In 34 women taking oral contraceptives were 8.88 ± 4.73 and 79.53 ± 30.46 ng/ml., respectively. In 35 pregnancy who have never taken oral contraceptives before pregnancy were 8.91 ± 5.17 and 98.44 ± 38.60 ng/ml., respectively and in 31 pregnancy who had taken oral contraceptives before pregnancy were 8.50 ± 4.82 and 76.03 ± 20.29 mg/ml., respectively. This study showed the folate levels in serum and red blood cell of pregnancy and non-pregnancy taking oral contraceptives were significant lower than those in healthy women, (P&lt;0.05). This groups should be supplemented with folic acid.