cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.48370/ofd/momxvs
title: Stokłosa miękka (Bromus hordeaceus L.), 2832

description: Herbarium
DOI: 10.48370/ofd/momxvs/9c36rs
title: HMW_Bromus_hordeaceus_L_H_2832.jpg

description: :unav
DOI: 10.48370/ofd/yjssqi
title: Stokłosa miękka (Bromus hordeaceus L.), 2834

description: Herbarium
DOI: 10.48370/ofd/yjssqi/iqgueg
title: HMW_Bromus_hordeaceus_L_H_2834.jpg

description: :unav
DOI: 10.48370/ofd/c26qvu
title: Stokłosa miękka (Bromus hordeaceus L.), 4239

description: Herbarium
DOI: 10.48370/ofd/c26qvu/m1rzxp
title: HMW_Bromus_hordeaceus_L_H_4239.jpg

description: :unav
DOI: 10.48370/ofd/uaplfo
title: Stokłosa miękka (Bromus hordeaceus L.), 4765

description: Herbarium
DOI: 10.48370/ofd/uaplfo/o4coof
title: HMW_Bromus_hordeaceus_L_H_4765.jpg

description: :unav
DOI: 10.48370/ofd/aetenz
title: Stokłosa miękka (Bromus hordeaceus L.), 3862

description: Herbarium
DOI: 10.48370/ofd/aetenz/1a5xlg
title: HMW_Bromus_hordeaceus_L_H_3862.jpg

description: :unav
DOI: 10.48370/ofd/sioxoe
title: Stokłosa miękka (Bromus hordeaceus L.), 4833

description: Herbarium
DOI: 10.48370/ofd/sioxoe/coezt7
title: HMW_Bromus_hordeaceus_L_H_4833.jpg

description: :unav
DOI: 10.48370/ofd/hfsudd
title: Stokłosa miękka (Bromus hordeaceus L.), 4874

description: Herbarium
DOI: 10.48370/ofd/hfsudd/r1ahaa
title: HMW_Bromus_hordeaceus_L_H_4874.jpg

description: :unav
DOI: 10.48370/ofd/tmpsgr
title: Stokłosa miękka (Bromus hordeaceus L.), 7261

description: Herbarium
DOI: 10.48370/ofd/tmpsgr/3svrjd
title: HMW_Bromus_hordeaceus_L_H_7261.jpg

description: :unav
DOI: 10.48370/ofd/qiszpk
title: Stokłosa miękka (Bromus hordeaceus L.), 4875

description: Herbarium
DOI: 10.48370/ofd/qiszpk/9naiio
title: HMW_Bromus_hordeaceus_L_H_4875.jpg

description: :unav
DOI: 10.48370/ofd/udhl6r
title: Stokłosa miękka (Bromus hordeaceus L.), 7267

description: Herbarium
DOI: 10.48370/ofd/udhl6r/lzp7ti
title: HMW_Bromus_hordeaceus_L_H_7267.jpg

description: :unav
DOI: 10.48370/ofd/pqxdnf
title: Stokłosa miękka (Bromus hordeaceus L.), 7939

description: Herbarium
DOI: 10.48370/ofd/pqxdnf/lor1kx
title: HMW_Bromus_hordeaceus_L_H_7939.jpg

description: :unav
DOI: 10.48370/ofd/eidkh2
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 3205

description: Herbarium
DOI: 10.48370/ofd/eidkh2/kb73xc
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_3205.jpg

description: :unav
DOI: 10.48370/ofd/cg86jg
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 2983

description: Herbarium
DOI: 10.48370/ofd/cg86jg/akdw98
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_2983.jpg

description: :unav
DOI: 10.48370/ofd/l1x3ys
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 4902

description: Herbarium
DOI: 10.48370/ofd/l1x3ys/u8xz6h
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_4902.jpg

description: :unav
DOI: 10.48370/ofd/bksazu
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 4961

description: Herbarium
DOI: 10.48370/ofd/bksazu/ep6te5
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_4961.jpg

description: :unav
DOI: 10.48370/ofd/fhjt1u
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 6922

description: Herbarium
DOI: 10.48370/ofd/fhjt1u/qjgcs6
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_6922.jpg

description: :unav
DOI: 10.48370/ofd/k2y5uw
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 4429

description: Herbarium
DOI: 10.48370/ofd/k2y5uw/ehequ2
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_4429.jpg

description: :unav
DOI: 10.48370/ofd/3ruls0
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 7881

description: Herbarium
DOI: 10.48370/ofd/3ruls0/9ennhq
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_7881.jpg

description: :unav
DOI: 10.17632/s25nk43cx5.1
title: Data for: Impact of socioeconomic status on survival following ST-elevation myocardial infarction in a universal healthcare system

description: Database
DOI: 10.48370/ofd/kcnpia
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 5338

description: Herbarium
DOI: 10.48370/ofd/kcnpia/vkwfn5
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_5338.jpg

description: :unav
DOI: 10.48370/ofd/0ejcv7
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 7936

description: Herbarium
DOI: 10.48370/ofd/0ejcv7/w030pa
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_7936.jpg

description: :unav
DOI: 10.48370/ofd/diynw9
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 8582

description: Herbarium
DOI: 10.48370/ofd/diynw9/v4wyo7
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_8582.jpg

description: :unav
DOI: 10.48370/ofd/xzhxho
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 8431

description: Herbarium
DOI: 10.48370/ofd/xzhxho/ssdhbe
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_8431.jpg

description: :unav
DOI: 10.48370/ofd/nvrxbe
title: Rajgras wyniosły (Arrhenatherum elatius (L.) P.Beauv. ex J.Presl C.Presl), 8591

description: Herbarium
DOI: 10.48370/ofd/nvrxbe/juxv4a
title: HMW_Arrhenatherum_elatius_L_P_Beauv_ex_J_Presl_&_C_Presl_H_8591.jpg

description: :unav
DOI: 10.48370/ofd/v6tyxn
title: Rdest ostrogorzki (Polygonum hydropiper L.), 8817

description: Herbarium
DOI: 10.48370/ofd/v6tyxn/otqadz
title: HMW_Polygonum_hydropiper_L_H_8817.jpg

description: :unav
DOI: 10.48370/ofd/xenh83
title: Rdest ostrogorzki (Polygonum hydropiper L.), 8690

description: Herbarium
DOI: 10.48370/ofd/xenh83/ftbmrv
title: HMW_Polygonum_hydropiper_L_H_8690.jpg

description: :unav
DOI: 10.48370/ofd/arpadj
title: Rdest ostrogorzki (Polygonum hydropiper L.), 8167

description: Herbarium
DOI: 10.48370/ofd/arpadj/wyfssu
title: HMW_Polygonum_hydropiper_L_H_8167.jpg

description: :unav
DOI: 10.48370/ofd/vuawzz
title: Rdest ostrogorzki (Polygonum hydropiper L.), 7502

description: Herbarium
DOI: 10.48370/ofd/vuawzz/88b5eo
title: HMW_Polygonum_hydropiper_L_H_7502.jpg

description: :unav
DOI: 10.48370/ofd/oc4qmh
title: Rdest ostrogorzki (Polygonum hydropiper L.), 6693

description: Herbarium
DOI: 10.48370/ofd/oc4qmh/whhfao
title: HMW_Polygonum_hydropiper_L_H_6693.jpg

description: :unav
DOI: 10.48370/ofd/30ohny
title: Rdest ostrogorzki (Polygonum hydropiper L.), 2043

description: Herbarium
DOI: 10.48370/ofd/30ohny/aieqho
title: HMW_Polygonum_hydropiper_L_H_2043.jpg

description: :unav
DOI: 10.48370/ofd/pspwtb
title: Rdest ostrogorzki (Polygonum hydropiper L.), 2039

description: Herbarium
DOI: 10.48370/ofd/pspwtb/36tigo
title: HMW_Polygonum_hydropiper_L_H_2039.jpg

description: :unav
DOI: 10.48370/ofd/7txgkl
title: Rdest ostrogorzki (Polygonum hydropiper L.), 3547

description: Herbarium
DOI: 10.48370/ofd/7txgkl/hgczoq
title: HMW_Polygonum_hydropiper_L_H_3547.jpg

description: :unav
DOI: 10.48370/ofd/ob6pcp
title: Rdest ostrogorzki (Polygonum hydropiper L.), 1523

description: Herbarium
DOI: 10.48370/ofd/ob6pcp/zqk5ld
title: HMW_Polygonum_hydropiper_L_H_1523.jpg

description: :unav
DOI: 10.48370/ofd/mbhcac
title: Rdest ostrogorzki (Polygonum hydropiper L.), 916

description: Herbarium
DOI: 10.48370/ofd/mbhcac/d4a82b
title: HMW_Polygonum_hydropiper_L_H_0916.jpg

description: :unav
DOI: 10.48370/ofd/k4udu8
title: Rdest ostrogorzki (Polygonum hydropiper L.), 7447

description: Herbarium
DOI: 10.48370/ofd/k4udu8/eijzbc
title: HMW_Polygonum_hydropiper_L_H_7447.jpg

description: :unav
DOI: 10.48370/ofd/iijckg
title: Rdest ostrogorzki (Polygonum hydropiper L.), 2042

description: Herbarium
DOI: 10.48370/ofd/iijckg/trggcc
title: HMW_Polygonum_hydropiper_L_H_2042.jpg

description: :unav
DOI: 10.48370/ofd/qkkfol
title: Rdest ostrogorzki (Polygonum hydropiper L.), 3657

description: Herbarium
DOI: 10.48370/ofd/qkkfol/vwbbzx
title: HMW_Polygonum_hydropiper_L_H_3657.jpg

description: :unav
DOI: 10.48370/ofd/ucivi1
title: Rdest ostrogorzki (Polygonum hydropiper L.), 2041

description: Herbarium
DOI: 10.48370/ofd/ucivi1/qzhx3f
title: HMW_Polygonum_hydropiper_L_H_2041.jpg

description: :unav
DOI: 10.6084/m9.figshare.13643147
title: Supplementary Information of "Guidelines for biomarker discovery in endometrium: Correcting for menstrual cycle bias reveals new uterine disorders associated genes"

description: <b>Table SII. Top uterine disorder biomarkers previously masked by the menstrual cycle effect. </b>Gene symbol, average expression, t-Student statistic, p-value, false discovery rate (FDR), fold change (FC) and absolute percentage of increased/decreased expression between cases and controls after differential expression analysis with menstrual cycle effect correction are provided for the top 20 (in terms of absolute FC) endometrial biomarkers of A) eutopic endometriosis (Burney2007, n=685), B) ectopic ovarian endometriosis (Hawkins2011, n=302), and C) recurrent implantation failure (Koot2016, n=30) that were previously masked by the menstrual cycle effect. Included new uterine disorder biomarkers (544 for eutopic endometriosis, 158 for ectopic ovarian endometriosis, and 27 for RIF) that were not previously reported in the original articles or in the databases Disgenet v.6 (Piñero <i>et al.</i>, 2019), Phenopedia v.6.2.3 (Yu <i>et al.</i>, 2010), and/or GeneCards v.4.14.0 (Stelzer <i>et al.</i>, 2016) are underlined.<b>Table SIII. Functional annotation for the newly discovered potential uterine disorder biomarkers. </b>For the new potential biomarkers of eutopic endometriosis (n=544) (B), ectopic endometriosis (n=158) (C) and RIF (n=27) (D) not previously reported in the literature, gene names, GeneCards description (Stelzer <i>et al.</i>, 2016), annotated GO biological processes, molecular functions and cellular components (Ashburner <i>et al</i>., 2000) and annotated KEGG pathways (Ogata <i>et al</i>., 1999) are indicated. For GO annotation, only experimental-evidenced GO-gene associations were included, a propagated GO version was used for considering the whole GO-tree structure, and annotated GO terms were filtered by those having more than five and less than 500 associated genes. Obtained GO and KEGG annotated terms were then grouped in broader functional categories (A). Table SIIIA shows, for each defined functional group, literature references supporting its role in the pathophysiology of endometriosis and/or RIF; together with the number of associated genes and gene names by study/uterine disorder. Keywords used in PubMed to search for functions altered in endometriosis and RIF patients included “endometriosis”, “RIF”, “recurrent implantation failure”, “function”, “pathway”, “gene ontology” and “KEGG”. GO, Gene Ontology. KEGG, Kyoto Encyclopedia of Genes and Genomes.
DOI: 10.5281/zenodo.4486586
title: Supplementary files from: Evolution of recombination landscapes in diverging populations of bread wheat

description: Supplementary files from: <strong>Evolution of recombination landscapes in diverging populations of bread wheat</strong> <strong>Abstract</strong> Reciprocal exchanges of DNA (crossovers) that occur during meiosis are mandatory to ensure the production of fertile gametes in sexually reproducing species. They also contribute to shuffle parental alleles into new combinations, thereby fuelling genetic variation and evolution. However, due to biological constraints, the recombination landscape is highly heterogenous along the genome, which limits the range of allelic combinations and the adaptability of populations. An approach to better understand the constraints on the recombination process is to study how it evolved in the past. In this work we tackled this question by constructing recombination profiles in four diverging bread wheat (<em>Triticum aestivum L.</em>) populations established from 371 landraces genotyped at 200,062 SNPs. We used linkage disequilibrium (LD) patterns to estimate in each population the past distribution of recombination along the genome and characterize its fine-scale heterogeneity. At the megabase scale, recombination rates derived from LD patterns were consistent with family-based estimates obtained from a population of 406 recombinant inbred lines. Among the four populations, recombination landscapes were positively correlated to each other and shared a statistically significant proportion of highly recombinant intervals. However, this comparison also highlighted that the similarity in recombination landscapes between populations was significantly decreasing with their genetic differentiation in most regions of the genome. This observation was found to be robust to SNP ascertainment and demography and suggests a relatively rapid evolution of factors determining the fine-scale localization of recombination in bread wheat.
DOI: 10.5281/zenodo.4486585
title: Supplementary files from: Evolution of recombination landscapes in diverging populations of bread wheat

description: Supplementary files from: <strong>Evolution of recombination landscapes in diverging populations of bread wheat</strong> <strong>Abstract</strong> Reciprocal exchanges of DNA (crossovers) that occur during meiosis are mandatory to ensure the production of fertile gametes in sexually reproducing species. They also contribute to shuffle parental alleles into new combinations, thereby fuelling genetic variation and evolution. However, due to biological constraints, the recombination landscape is highly heterogenous along the genome, which limits the range of allelic combinations and the adaptability of populations. An approach to better understand the constraints on the recombination process is to study how it evolved in the past. In this work we tackled this question by constructing recombination profiles in four diverging bread wheat (<em>Triticum aestivum L.</em>) populations established from 371 landraces genotyped at 200,062 SNPs. We used linkage disequilibrium (LD) patterns to estimate in each population the past distribution of recombination along the genome and characterize its fine-scale heterogeneity. At the megabase scale, recombination rates derived from LD patterns were consistent with family-based estimates obtained from a population of 406 recombinant inbred lines. Among the four populations, recombination landscapes were positively correlated to each other and shared a statistically significant proportion of highly recombinant intervals. However, this comparison also highlighted that the similarity in recombination landscapes between populations was significantly decreasing with their genetic differentiation in most regions of the genome. This observation was found to be robust to SNP ascertainment and demography and suggests a relatively rapid evolution of factors determining the fine-scale localization of recombination in bread wheat.
DOI: 10.48370/ofd/jfcc2f
title: Krzyżownica czubata (Polygala comosa Schkuhr), 269

description: Herbarium
DOI: 10.48370/ofd/jfcc2f/oal3aq
title: HMW_Polygala_comosa_Schkuhr_H_0269.jpg

description: :unav
DOI: 10.48370/ofd/0chdbl
title: Krzyżownica czubata (Polygala comosa Schkuhr), 3052

description: Herbarium
DOI: 10.48370/ofd/0chdbl/kwffql
title: HMW_Polygala_comosa_Schkuhr_H_3052.jpg

description: :unav
DOI: 10.48370/ofd/irdsy1
title: Krzyżownica czubata (Polygala comosa Schkuhr), 2349

description: Herbarium
DOI: 10.48370/ofd/irdsy1/e44csn
title: HMW_Polygala_comosa_Schkuhr_H_2349.jpg

description: :unav
DOI: 10.48370/ofd/n7epfx
title: Krzyżownica czubata (Polygala comosa Schkuhr), 3051

description: Herbarium
DOI: 10.48370/ofd/n7epfx/hvp0rn
title: HMW_Polygala_comosa_Schkuhr_H_3051.jpg

description: :unav
DOI: 10.48370/ofd/f2g9ve
title: Krzyżownica czubata (Polygala comosa Schkuhr), 3588

description: Herbarium
DOI: 10.48370/ofd/f2g9ve/93mpip
title: HMW_Polygala_comosa_Schkuhr_H_3588.jpg

description: :unav
DOI: 10.48370/ofd/rgdzgt
title: Krzyżownica czubata (Polygala comosa Schkuhr), 5339

description: Herbarium
DOI: 10.48370/ofd/rgdzgt/tpftwd
title: HMW_Polygala_comosa_Schkuhr_H_5339.jpg

description: :unav
DOI: 10.48370/ofd/jjweou
title: Krzyżownica czubata (Polygala comosa Schkuhr), 3213

description: Herbarium
DOI: 10.48370/ofd/jjweou/5cehjc
title: HMW_Polygala_comosa_Schkuhr_H_3213.jpg

description: :unav
DOI: 10.48370/ofd/vyj9kq
title: Krzyżownica czubata (Polygala comosa Schkuhr), 4842

description: Herbarium
DOI: 10.48370/ofd/vyj9kq/7z7d1z
title: HMW_Polygala_comosa_Schkuhr_H_4842.jpg

description: :unav
DOI: 10.48370/ofd/ceoltz
title: Krzyżownica czubata (Polygala comosa Schkuhr), 5358

description: Herbarium
DOI: 10.48370/ofd/ceoltz/cawsyv
title: HMW_Polygala_comosa_Schkuhr_H_5358.jpg

description: :unav
DOI: 10.48370/ofd/sr4evd
title: Krzyżownica czubata (Polygala comosa Schkuhr), 7276

description: Herbarium
DOI: 10.48370/ofd/sr4evd/r09u0v
title: HMW_Polygala_comosa_Schkuhr_H_7276.jpg

description: :unav
DOI: 10.48370/ofd/abuepk
title: Krzyżownica czubata (Polygala comosa Schkuhr), 5397

description: Herbarium
DOI: 10.48370/ofd/abuepk/mlvq2d
title: HMW_Polygala_comosa_Schkuhr_H_5397.jpg

description: :unav
DOI: 10.48370/ofd/aofua8
title: Krzyżownica czubata (Polygala comosa Schkuhr), 7392

description: Herbarium
DOI: 10.48370/ofd/aofua8/usucor
title: HMW_Polygala_comosa_Schkuhr_H_7392.jpg

description: :unav
DOI: 10.48370/ofd/2znlbl
title: Wierzbownica bladoróżowa (Epilobium roseum Schreb.), 2514

description: Herbarium
DOI: 10.48370/ofd/2znlbl/un1iah
title: HMW_Epilobium_roseum_Schreb_H_2514.jpg

description: :unav
DOI: 10.48370/ofd/xex5mi
title: Krzyżownica czubata (Polygala comosa Schkuhr), 8185

description: Herbarium
DOI: 10.48370/ofd/xex5mi/9o3auf
title: HMW_Polygala_comosa_Schkuhr_H_8185.jpg

description: :unav
DOI: 10.48370/ofd/cstjul
title: Wierzbownica bladoróżowa (Epilobium roseum Schreb.), 2573

description: Herbarium
DOI: 10.48370/ofd/cstjul/2bibaj
title: HMW_Epilobium_roseum_Schreb_H_2573.jpg

description: :unav
DOI: 10.48370/ofd/iigoep
title: Wierzbownica bladoróżowa (Epilobium roseum Schreb.), 3572

description: Herbarium
DOI: 10.48370/ofd/iigoep/ukdtdh
title: HMW_Epilobium_roseum_Schreb_H_3572.jpg

description: :unav
DOI: 10.48370/ofd/hd0ytt
title: Wierzbownica bladoróżowa (Epilobium roseum Schreb.), 3818

description: Herbarium
DOI: 10.48370/ofd/hd0ytt/rl3tnj
title: HMW_Epilobium_roseum_Schreb_H_3818.jpg

description: :unav
DOI: 10.5281/zenodo.4540605
title: Dataset related to article "Metformin and Everolimus: A Promising Combination for Neuroendocrine Tumors Treatment"

description: This record contains data related to article: Metformin and Everolimus: A Promising Combination for Neuroendocrine Tumors Treatment Introduction: Treatment options for neuroendocrine tumors (NETs) are rarely curative, as NETs frequently show resistance to medical therapy. The use of everolimus, an mTOR inhibitor, is limited by the development of resistance, probably due to the activation of Akt signaling. In this context, the antidiabetic drug metformin is able to inhibit mTOR, providing a rationale for the use of metformin and everolimus in combination. Methods: We investigated the effects of the metformin and everolimus combination on NET cell proliferation, apoptosis, colony formation, cell viability, NET spheroids growth and the involvement of the Akt and mTOR pathways, and also developed everolimus-resistant NET cells to further study this combination. Results: Metformin and everolimus in combination are more effective than monotherapy in inhibiting pancreatic NET (PAN-NET) cell proliferation (−71% ± 13%, <em>p</em> &lt; 0.0001 vs. basal), whereas no additive effects were observed on pulmonary neuroendocrine tumor (PNT) cell proliferation. The combinatorial treatment is more effective than monotherapy in inhibiting colony formation, cell viability, NET spheroids growth rate and mTOR phosphorylation in both NET cell lines. In a PAN-NET cell line, metformin did not affect Akt phosphorylation; conversely, it significantly decreased Akt phosphorylation in a PNT cell line. Using everolimus-resistant NET cells, we confirmed that metformin maintained its effects, acting by two different pathways: Akt-dependent or independent, depending on the cell type, with both leading to mTOR suppression. Conclusions: Considering the promising effects of the everolimus and metformin combination in NET cells, our results provide a rationale for its use in NET patients.
DOI: 10.5281/zenodo.4540604
title: Dataset related to article "Metformin and Everolimus: A Promising Combination for Neuroendocrine Tumors Treatment"

description: This record contains data related to article: Metformin and Everolimus: A Promising Combination for Neuroendocrine Tumors Treatment Introduction: Treatment options for neuroendocrine tumors (NETs) are rarely curative, as NETs frequently show resistance to medical therapy. The use of everolimus, an mTOR inhibitor, is limited by the development of resistance, probably due to the activation of Akt signaling. In this context, the antidiabetic drug metformin is able to inhibit mTOR, providing a rationale for the use of metformin and everolimus in combination. Methods: We investigated the effects of the metformin and everolimus combination on NET cell proliferation, apoptosis, colony formation, cell viability, NET spheroids growth and the involvement of the Akt and mTOR pathways, and also developed everolimus-resistant NET cells to further study this combination. Results: Metformin and everolimus in combination are more effective than monotherapy in inhibiting pancreatic NET (PAN-NET) cell proliferation (−71% ± 13%, <em>p</em> &lt; 0.0001 vs. basal), whereas no additive effects were observed on pulmonary neuroendocrine tumor (PNT) cell proliferation. The combinatorial treatment is more effective than monotherapy in inhibiting colony formation, cell viability, NET spheroids growth rate and mTOR phosphorylation in both NET cell lines. In a PAN-NET cell line, metformin did not affect Akt phosphorylation; conversely, it significantly decreased Akt phosphorylation in a PNT cell line. Using everolimus-resistant NET cells, we confirmed that metformin maintained its effects, acting by two different pathways: Akt-dependent or independent, depending on the cell type, with both leading to mTOR suppression. Conclusions: Considering the promising effects of the everolimus and metformin combination in NET cells, our results provide a rationale for its use in NET patients.
DOI: 10.48370/ofd/4ozpcu
title: Wierzbownica bladoróżowa (Epilobium roseum Schreb.), 4683

description: Herbarium
DOI: 10.48370/ofd/4ozpcu/54zqbx
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