cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.11123247
title: Single-cell transcriptomic profiling of human pancreatic islets reveals genes responsive to glucose exposure over 24 hours

description: Aims/hypothesis: Disruption of pancreatic islet function and glucose homeostasis can lead to the development of sustained hyperglycemia, beta cell glucotoxicity, and subsequently type 2 diabetes. In this study, we explored the effects of in vitro hyperglycemic conditions on human pancreatic islet gene expression across 24 hours in six pancreatic cell types: alpha, beta, gamma, delta, ductal, and acinar cells. We hypothesized that genes associated with hyperglycemic conditions may be relevant to the onset and progression of diabetes.

Methods: We exposed human pancreatic islets from two donors to low (2.8 mmol/l) and high (15.0 mmol/l) glucose concentrations over 24 hours in vitro. To assess the transcriptome, we performed single-cell RNA sequencing (scRNA-seq) at seven time points. We modeled time as both a discrete and continuous variable to determine momentary and longitudinal changes in transcription associated with islet time in culture or glucose exposure. Additionally, we integrated genomic features and genetic summary statistics to nominate candidate effector genes. For three of these genes, we functionally characterized the effect on insulin production and secretion using CRISPR interference to knockdown gene expression in EndoC-βH1 cells, followed by a glucose-stimulated insulin secretion assay.

Results: Across all cell types, we identified 1,447 genes associated with time, 680 genes associated with glucose exposure, and 418 genes associated with interaction effects between time and glucose. By integrating these expression profiles with summary statistics from genetic association studies, we identified 2,449 candidate effector genes for type 2 diabetes, HbA1c, random blood glucose, and fasting blood glucose. Of these candidate effector genes, we showed that three—ERO1B, HNRNPA2B1, and RHOBTB3—exhibited an effect on glucose-stimulated insulin secretion and production in EndoC-βH1 cells.

Conclusions/interpretation: The findings of our study provide an in-depth characterization of the 24-hour transcriptomic response of human pancreatic islets to glucose exposure at a single-cell resolution. By integrating differentially expressed genes with genetic signals for type 2 diabetes and glucose-related traits, we provide insights into the molecular mechanisms underlying glucose homeostasis. Finally, we provide functional evidence to support the role of three candidate effector genes in insulin secretion and production.
DOI: 10.5281/zenodo.11123248
title: Single-cell transcriptomic profiling of human pancreatic islets reveals genes responsive to glucose exposure over 24 hours

description: Aims/hypothesis: Disruption of pancreatic islet function and glucose homeostasis can lead to the development of sustained hyperglycemia, beta cell glucotoxicity, and subsequently type 2 diabetes. In this study, we explored the effects of in vitro hyperglycemic conditions on human pancreatic islet gene expression across 24 hours in six pancreatic cell types: alpha, beta, gamma, delta, ductal, and acinar cells. We hypothesized that genes associated with hyperglycemic conditions may be relevant to the onset and progression of diabetes.

Methods: We exposed human pancreatic islets from two donors to low (2.8 mmol/l) and high (15.0 mmol/l) glucose concentrations over 24 hours in vitro. To assess the transcriptome, we performed single-cell RNA sequencing (scRNA-seq) at seven time points. We modeled time as both a discrete and continuous variable to determine momentary and longitudinal changes in transcription associated with islet time in culture or glucose exposure. Additionally, we integrated genomic features and genetic summary statistics to nominate candidate effector genes. For three of these genes, we functionally characterized the effect on insulin production and secretion using CRISPR interference to knockdown gene expression in EndoC-βH1 cells, followed by a glucose-stimulated insulin secretion assay.

Results: Across all cell types, we identified 1,447 genes associated with time, 680 genes associated with glucose exposure, and 418 genes associated with interaction effects between time and glucose. By integrating these expression profiles with summary statistics from genetic association studies, we identified 2,449 candidate effector genes for type 2 diabetes, HbA1c, random blood glucose, and fasting blood glucose. Of these candidate effector genes, we showed that three—ERO1B, HNRNPA2B1, and RHOBTB3—exhibited an effect on glucose-stimulated insulin secretion and production in EndoC-βH1 cells.

Conclusions/interpretation: The findings of our study provide an in-depth characterization of the 24-hour transcriptomic response of human pancreatic islets to glucose exposure at a single-cell resolution. By integrating differentially expressed genes with genetic signals for type 2 diabetes and glucose-related traits, we provide insights into the molecular mechanisms underlying glucose homeostasis. Finally, we provide functional evidence to support the role of three candidate effector genes in insulin secretion and production.
DOI: 10.15468/dl.pdztv3
title: Occurrence Download

description: A dataset containing 226 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is one of (Cerastium nemorale M.Bieb.)"
 ]
}

 The dataset includes 226 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008874-240506114902167/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.zz56g6
title: Occurrence Download

description: A dataset containing 48475 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is one of (Cerastium tomentosum L.)"
 ]
}

 The dataset includes 48475 records from 492 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008875-240506114902167/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6q43a7
title: Occurrence Download

description: A dataset listing the 38471 species recorded in GBIF matching the query:
{
 "Country" : [
 "is Sri Lanka"
 ]
}

 The dataset's 38471 records were derived from 1130 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0180866-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rqstjt
title: Occurrence Download

description: A dataset containing 292 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 292 records from 48 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0194766-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9f3s3s
title: Occurrence Download

description: A dataset containing 216377 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Zosteraceae",
 "HasCoordinate is true"
 ]
}

 The dataset includes 216377 records from 463 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0180862-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.lswmul
title: Occurrence Download

description: A dataset containing 8165 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Enteropogon acicularis (Lindl.) Lazarides",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 8165 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000469-170925132151566/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.1flwhr
title: Occurrence Download

description: A dataset containing 302 species occurrences available in GBIF matching the query:
{
 "and" : [
 "InstitutionCode is SANT",
 "TaxonKey is Jasione montana L."
 ]
}

 The dataset includes 302 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011037-191105090559680/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.s9wu7x
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Spiraea robusta Hand.-Mazz."
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117576-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bfq7nw
title: Occurrence Download

description: A dataset containing 17 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tachiadenus antaisaka Humbert"
 ]
}

 The dataset includes 17 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117577-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.g3fp4n
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Stenogyne calycosa Sherff"
 ]
}

 The dataset includes 21 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117578-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.y94x2k
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Syzygium cardiophyllum (Merr.) Merr."
 ]
}

 The dataset includes 4 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117579-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ec62zv
title: Occurrence Download

description: A dataset containing 1741 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Symplocos adenophylla Wall. ex G.Don"
 ]
}

 The dataset includes 1741 records from 50 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117580-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wn643p
title: Occurrence Download

description: A dataset containing 1039 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Spiraea rosthornii E.Pritz."
 ]
}

 The dataset includes 1039 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117581-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9t6vzf
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tachiadenus boivinii Humbert"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117582-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nrmqnx
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Syzygium caroli Diels"
 ]
}

 The dataset includes 2 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117584-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2evup6
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Stenogyne campanulata Weller & Sakai"
 ]
}

 The dataset includes 21 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117583-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.djqd74
title: Occurrence Download

description: A dataset containing 650 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Symplocos adenopus Hance"
 ]
}

 The dataset includes 650 records from 28 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117585-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ytsmj4
title: Occurrence Download

description: A dataset containing 487 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tachiadenus carinatus (Desr.) Griseb."
 ]
}

 The dataset includes 487 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117586-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5gcu5n
title: Occurrence Download

description: A dataset containing 113 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Syzygium carolinense (Koidz.) Hosok."
 ]
}

 The dataset includes 113 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117587-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.g9z74a
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Stenogyne cinerea Hillebr."
 ]
}

 The dataset includes 2 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117589-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.mnfhpt
title: Occurrence Download

description: A dataset containing 14323 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Spiraea salicifolia L."
 ]
}

 The dataset includes 14323 records from 416 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117588-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2t7ccw
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Symplocos ambangensis Noot."
 ]
}

 The dataset includes 3 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117591-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.reuaaf
title: Occurrence Download

description: A dataset containing 27 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Stenogyne cranwelliae Sherff"
 ]
}

 The dataset includes 27 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117592-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dcnr62
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Syzygium carrii T.G.Hartley & L.M.Perry"
 ]
}

 The dataset includes 5 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117594-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.u877nf
title: Occurrence Download

description: A dataset containing 189 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tachiadenus gracilis Griseb."
 ]
}

 The dataset includes 189 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117593-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vqttfv
title: Occurrence Download

description: A dataset containing 103 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Spiraea sargentiana Rehder"
 ]
}

 The dataset includes 103 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117595-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ms89tc
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Symplocos anamallayana Bedd."
 ]
}

 The dataset includes 4 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117596-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.suapeu
title: Occurrence Download

description: A dataset containing 27 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Stenogyne haliakalae Wawra"
 ]
}

 The dataset includes 27 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117597-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vjm5rv
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Syzygium cartilagineum Merr. & L.M.Perry"
 ]
}

 The dataset includes 3 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117598-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ha78ep
title: Occurrence Download

description: A dataset containing 912703335 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is North America",
 "Country is United States of America",
 "OccurrenceStatus is Present",
 "Year 1734-2024"
 ]
}

 The dataset includes 912703335 records from 2989 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0117527-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.29xkyn
title: Occurrence Download

description: A dataset containing 5262 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Kuhliidae"
 ]
}

 The dataset includes 5262 records from 130 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0055796-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.pkesfr
title: Occurrence Download

description: A dataset containing 10142 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Tangara gyrola (Linnaeus, 1758)",
 "HasGeospatialIssue is false",
 "HasCoordinate is true"
 ]
}

 The dataset includes 10142 records from 44 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002969-150922153815467/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bec85j
title: Occurrence Download

description: A dataset containing 599063 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Pycnonotus sinensis (Gmelin, 1789)"
 ]
}

 The dataset includes 599063 records from 96 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048561-231002084531237/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bp7k2h
title: Occurrence Download

description: A dataset containing 51 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Parus pallidiventris Reichenow, 1885"
 ]
}

 The dataset includes 51 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0055789-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ts8ptg
title: Occurrence Download

description: A dataset containing 21480 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Olyra L."
 ]
}

 The dataset includes 21480 records from 433 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0049584-231002084531237/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.egw0ek
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Dypsis vonitrandambo Rakotoarin. & J.Dransf."
 ]
}

 The dataset includes 3 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002969-160623103540712/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.x89gwh
title: Occurrence Download

description: A dataset containing 414 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-28.71360 30.82233,59.71360 30.82233,59.71360 66.17767,-28.71360 66.17767,-28.71360 30.82233))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Cheilosia soror (Zetterstedt, 1843)"
 ]
}

 The dataset includes 414 records from 46 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0063577-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.k7w2rs
title: Occurrence Download

description: A dataset containing 108 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tetramicra tenera (A.Rich.) Griseb. ex Benth."
 ]
}

 The dataset includes 108 records from 24 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0193650-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.