cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15156/bio/sh0826389.10fu
title: SH0826389.10FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15151/esrf-es-1018398452
title: The Israeli BAG

description: The BAG is quite large with 24 principal investigators covering almost all groups in Israel. The scientific topics are diverse and so different that depicting them in a short Abstract is extremely difficult. In this context BAG members are studying the parameters that govern protein assemblage, structural impacts of signaling processes in different cascades. There are several projects that are directed towards molecular recognition with implementation towards drug design. In addition, we still have the ribosome project and the design of unique antibiotics with specific functional interactions from the mycinamycins family and their resistance related mechanism compared with other macrolides and the photosystem and how it converts light into energy. Since Covid-19 is still a relevant topic as are other viral infections BAG members are and have been involved in the understanding of the infection process and the means to hinder it.
DOI: 10.15151/esrf-es-1025803720
title: Scanning and In-situ MLL Development for X-ray Nanodiffraction on Thin Film, Space and Additively Manufactured Applications
DOI: 10.6084/m9.figshare.21936622
title: Attitude to death and opinion of medical students on the top training

description: Abstract: Introduction: Currently, most people are inserted in a sociohistorical context of denial of death, including physicians who deal with dying daily who consider themselves unprepared in the face of the death of a patient. The discomfort of these professionals in the face of the death process is due in part to the lack of the training that occurred during graduation. Objective: This study aimed to analyze the opinion of medical students at the Faculdade Pernambucana de Saúde (FPS) about teaching and perceptions on death and dying during graduation, verify the importance in medical education and describe this approach through the students’ perspective. It is also objective to find aspects that influence the belief of undergraduates about the theme. Method: This is an exploratory cross-sectional study with quantitative method, conducted between August 2020 and July 2021. Data collection was carried out through online questionnaires, with a sample of 365 subjects, after acceptance and marking on the free and informed consent form, sent to students by e-mail. For analysis, epi-info software was used using relative and absolute frequencies to describe qualitative variables and position and dispersion measures to describe quantitative variables. The chi-square test was used to verify the chi-square test, considering a significance level of 5%. The study followed all the precepts of Resolution nº 510/2016 of the National Council of Ethics in Research and received approval from the ethics committee of the college under opinion number 4,228,016. Result: Most students associate anguish, fear, and sadness with the process of death and agree that the discussion of this process in graduation impacts on the practice of medicine and on the psychosocial well-being of the physician. The students agree that the approach to the death process happened in rare moments during graduation and consider it necessary to include more disciplines that address it. Conclusion: The discussion about the process of death and dying process during medical graduation is necessary, and the insertion of modules that approach it is recommended in the medical course of FPS to contribute to the students’ understanding of this process and in the psychological preparation of these students to deal with death in the exercise of the profession.
DOI: 10.6084/m9.figshare.21936622.v1
title: Attitude to death and opinion of medical students on the top training

description: Abstract: Introduction: Currently, most people are inserted in a sociohistorical context of denial of death, including physicians who deal with dying daily who consider themselves unprepared in the face of the death of a patient. The discomfort of these professionals in the face of the death process is due in part to the lack of the training that occurred during graduation. Objective: This study aimed to analyze the opinion of medical students at the Faculdade Pernambucana de Saúde (FPS) about teaching and perceptions on death and dying during graduation, verify the importance in medical education and describe this approach through the students’ perspective. It is also objective to find aspects that influence the belief of undergraduates about the theme. Method: This is an exploratory cross-sectional study with quantitative method, conducted between August 2020 and July 2021. Data collection was carried out through online questionnaires, with a sample of 365 subjects, after acceptance and marking on the free and informed consent form, sent to students by e-mail. For analysis, epi-info software was used using relative and absolute frequencies to describe qualitative variables and position and dispersion measures to describe quantitative variables. The chi-square test was used to verify the chi-square test, considering a significance level of 5%. The study followed all the precepts of Resolution nº 510/2016 of the National Council of Ethics in Research and received approval from the ethics committee of the college under opinion number 4,228,016. Result: Most students associate anguish, fear, and sadness with the process of death and agree that the discussion of this process in graduation impacts on the practice of medicine and on the psychosocial well-being of the physician. The students agree that the approach to the death process happened in rare moments during graduation and consider it necessary to include more disciplines that address it. Conclusion: The discussion about the process of death and dying process during medical graduation is necessary, and the insertion of modules that approach it is recommended in the medical course of FPS to contribute to the students’ understanding of this process and in the psychological preparation of these students to deal with death in the exercise of the profession.
DOI: 10.6084/m9.figshare.21936621
title: Physico-Chemical Parameters, Oxidative Stress, and Fatty Acid Profile of American Pekin Ducks (Anas Platyrhynchos Domesticus) Raised under Different Production Systems

description: ABSTRACT Rearing of American Pekin ducks in different production systems plays a vital role in the determination of the internal climatic conditions of the house for optimum health and meat quality parameters. The experiment was designed to evaluate the influence of different rearing systems fed on kitchen waste on meat quality parameters of American Pekin ducks. A total of 180 ducklings (10 days old) were distributed randomly into three experimental groups; intensive production system (IPS), free-range production system (FRPS), and pool with yard production system (PYPS). We investigate the physicochemical parameters, meat coloration, oxidative stress, and fatty acid (FA) profile of Pekin ducks. Ducks reared in PYPS showed better physico-chemical parameters and meat coloration than those of ducks reared in IPS and FRPS. Drip loss % and cooking loss % are significantly low in FPRS while (L*) l-lightness, (a*) redness, and (b*) yellowness are significantly higher in IPS (p≤0.05). The oxidative stress was reduced in PYPS due to the natural behavior of ducks. Moreover, the fatty acid profile was improved in PYPS fed with 100% kitchen waste. In conclusion, this experiment confirmed that ducks reared in PYPS improve their meat quality parameters, fatty acid, and oxidative stress.
DOI: 10.6084/m9.figshare.21936621.v1
title: Physico-Chemical Parameters, Oxidative Stress, and Fatty Acid Profile of American Pekin Ducks (Anas Platyrhynchos Domesticus) Raised under Different Production Systems

description: ABSTRACT Rearing of American Pekin ducks in different production systems plays a vital role in the determination of the internal climatic conditions of the house for optimum health and meat quality parameters. The experiment was designed to evaluate the influence of different rearing systems fed on kitchen waste on meat quality parameters of American Pekin ducks. A total of 180 ducklings (10 days old) were distributed randomly into three experimental groups; intensive production system (IPS), free-range production system (FRPS), and pool with yard production system (PYPS). We investigate the physicochemical parameters, meat coloration, oxidative stress, and fatty acid (FA) profile of Pekin ducks. Ducks reared in PYPS showed better physico-chemical parameters and meat coloration than those of ducks reared in IPS and FRPS. Drip loss % and cooking loss % are significantly low in FPRS while (L*) l-lightness, (a*) redness, and (b*) yellowness are significantly higher in IPS (p≤0.05). The oxidative stress was reduced in PYPS due to the natural behavior of ducks. Moreover, the fatty acid profile was improved in PYPS fed with 100% kitchen waste. In conclusion, this experiment confirmed that ducks reared in PYPS improve their meat quality parameters, fatty acid, and oxidative stress.
DOI: 10.6084/m9.figshare.21936625
title: Acquisition of endoscopic nasal surgery skills with a lamb’s head model

description: Abstract Objectives: To evaluate the acquisition of surgical skills by otolaryngology residents and established the minimum number of dissections of a lamb’s model to be performed before practicing on human patients. Methods: Nineteen second-year otolaryngology residents performed ten dissections each, five on each nasal cavity, always practicing the same three surgical procedures on the lamb model. Each student’s training lasted 2-months, and the entire training intervention lasted 4-years, over four generations of residents. All dissections were recorded and were selected at random for examination by two independent otolaryngology surgeons, who were otherwise not involved in the research. Assessment of the 190 dissections used an instrument validated for surgical training of medical residents. Results: To a 1% significance level, statistical analysis revealed increased performance and satisfactory results were observed after the sixth dissection. Furthermore, after the eighth dissection, skill acquisition was relevant and sustained. Conclusion: Training in endoscopic nasal surgery on a lamb’s head model improves surgical skills and handling of surgical instruments. Our results showed the relevance of the lamb model for training in otolaryngology surgery, impacting on patient safety. Level of evidence: 2.
DOI: 10.6084/m9.figshare.21936625.v1
title: Acquisition of endoscopic nasal surgery skills with a lamb’s head model

description: Abstract Objectives: To evaluate the acquisition of surgical skills by otolaryngology residents and established the minimum number of dissections of a lamb’s model to be performed before practicing on human patients. Methods: Nineteen second-year otolaryngology residents performed ten dissections each, five on each nasal cavity, always practicing the same three surgical procedures on the lamb model. Each student’s training lasted 2-months, and the entire training intervention lasted 4-years, over four generations of residents. All dissections were recorded and were selected at random for examination by two independent otolaryngology surgeons, who were otherwise not involved in the research. Assessment of the 190 dissections used an instrument validated for surgical training of medical residents. Results: To a 1% significance level, statistical analysis revealed increased performance and satisfactory results were observed after the sixth dissection. Furthermore, after the eighth dissection, skill acquisition was relevant and sustained. Conclusion: Training in endoscopic nasal surgery on a lamb’s head model improves surgical skills and handling of surgical instruments. Our results showed the relevance of the lamb model for training in otolaryngology surgery, impacting on patient safety. Level of evidence: 2.
DOI: 10.6084/m9.figshare.21936628
title: Feasibility of a snowball sampling survey to study active surveillance for thyroid microcarcinoma treatment among endocrinologists and surgeons of Brazil

description: Abstract Objectives: This study aims to investigate if a sampling method using virtual networks is feasible to survey AS adoption among this “hard-to-reach” population of Brazilian doctors. Methods: An online piloted 11-point structured survey questionnaire (designed using Googleforms®) probed the actual treatment patterns for adult patients with PTMCs, including treatment decision-making nonoperative options, was undertaken between 10 November and 30 November 2020. Participants were reached by the mobile phone Application (APP) and a snowball sampling strategy was used to recruit a total of 4783 members (maximum number of potential reach), which is the total of doctors of the all 21 social media WhatsApp® groups. Results: From a total of 4783 members (maximum number of potential reach), there were 657 (13.7%) doctors (actual reach) who clicked the web link of the questionnaire, out of whom 512 (10.7%) fully completed the online survey. Among the survey respondents, 361 were endocrinologists (70.5%) and 151 were surgeons (29.5%). Overall, for low-risk PTMCs in an elderly patient, 118 responders (23%) recommend AS, while 390 (76%) recommend immediate surgery as the management, including lobectomy (18.5%) and Total Thyroidectomy (58.2%). The present responders tended to recommend surgery for PTMCs that were located adjacent to the dorsal surface of the thyroid, were multiple, or raised the size during the follow-up. Conclusion: Using snowball sampling strategy as an innovative route to conduct surveys was feasible and applicable but the rate of response was still very low. Our data also suggests the need to investigate if AS is embraced by Brazilian doctors.
DOI: 10.6084/m9.figshare.21936628.v1
title: Feasibility of a snowball sampling survey to study active surveillance for thyroid microcarcinoma treatment among endocrinologists and surgeons of Brazil

description: Abstract Objectives: This study aims to investigate if a sampling method using virtual networks is feasible to survey AS adoption among this “hard-to-reach” population of Brazilian doctors. Methods: An online piloted 11-point structured survey questionnaire (designed using Googleforms®) probed the actual treatment patterns for adult patients with PTMCs, including treatment decision-making nonoperative options, was undertaken between 10 November and 30 November 2020. Participants were reached by the mobile phone Application (APP) and a snowball sampling strategy was used to recruit a total of 4783 members (maximum number of potential reach), which is the total of doctors of the all 21 social media WhatsApp® groups. Results: From a total of 4783 members (maximum number of potential reach), there were 657 (13.7%) doctors (actual reach) who clicked the web link of the questionnaire, out of whom 512 (10.7%) fully completed the online survey. Among the survey respondents, 361 were endocrinologists (70.5%) and 151 were surgeons (29.5%). Overall, for low-risk PTMCs in an elderly patient, 118 responders (23%) recommend AS, while 390 (76%) recommend immediate surgery as the management, including lobectomy (18.5%) and Total Thyroidectomy (58.2%). The present responders tended to recommend surgery for PTMCs that were located adjacent to the dorsal surface of the thyroid, were multiple, or raised the size during the follow-up. Conclusion: Using snowball sampling strategy as an innovative route to conduct surveys was feasible and applicable but the rate of response was still very low. Our data also suggests the need to investigate if AS is embraced by Brazilian doctors.
DOI: 10.6084/m9.figshare.21936627
title: Prognostic importance of expression of mini-chromosome maintenance proteins (MCMs) in patients with nasopharyngeal cancer treated with curative radiotherapy

description: Abstract Objective: The prognostic importance of minichromosome maintenance complex expression in nasopharyngeal cancer is still unknown. We aimed to find whether minichromosome maintenance complex 2–7 expression may potentially be used to predict the prognosis of nasopharyngeal cancer patients treated with definitive radiotherapy. Methods: Between April 2007 and July 2020, patients with nasopharyngeal cancer treated with radiotherapy were identified. Immunohistochemical analysis was performed on formalin-fixed paraffin-embedded tissues of cases. A single pathologist analyzed the histologic specimens of all patients. Results: Totally, 67 patients were included. The median followup was 75.3 months. Higher tumor (T) stage was correlated with minichromosome maintenance complex 2 overexpression. Minichromosome maintenance complex s expression was also associated with histopathologic subgroups. According to univariate analysis, AJCC stage, histopathological subgroups, tumor response after treatment, minichromosome maintenance complex 2, 3, 5, 6 and 7 expression were the prognostic factors that predict overall survival. According to multivariate analysis minichromosome maintenance complex 7 expression was the only prognostic marker for both progression-free survival and overall survival. Conclusion: The overexpression of minichromosome maintenance complex 2, 3, 5, 6 and 7 indicated bad prognosis. Minichromosome maintenance complex 7 was an independent prognostic factor for survival outcomes in nasopharyngeal cancer and may be a potential therapeutic target for treatment.
DOI: 10.6084/m9.figshare.21936627.v1
title: Prognostic importance of expression of mini-chromosome maintenance proteins (MCMs) in patients with nasopharyngeal cancer treated with curative radiotherapy

description: Abstract Objective: The prognostic importance of minichromosome maintenance complex expression in nasopharyngeal cancer is still unknown. We aimed to find whether minichromosome maintenance complex 2–7 expression may potentially be used to predict the prognosis of nasopharyngeal cancer patients treated with definitive radiotherapy. Methods: Between April 2007 and July 2020, patients with nasopharyngeal cancer treated with radiotherapy were identified. Immunohistochemical analysis was performed on formalin-fixed paraffin-embedded tissues of cases. A single pathologist analyzed the histologic specimens of all patients. Results: Totally, 67 patients were included. The median followup was 75.3 months. Higher tumor (T) stage was correlated with minichromosome maintenance complex 2 overexpression. Minichromosome maintenance complex s expression was also associated with histopathologic subgroups. According to univariate analysis, AJCC stage, histopathological subgroups, tumor response after treatment, minichromosome maintenance complex 2, 3, 5, 6 and 7 expression were the prognostic factors that predict overall survival. According to multivariate analysis minichromosome maintenance complex 7 expression was the only prognostic marker for both progression-free survival and overall survival. Conclusion: The overexpression of minichromosome maintenance complex 2, 3, 5, 6 and 7 indicated bad prognosis. Minichromosome maintenance complex 7 was an independent prognostic factor for survival outcomes in nasopharyngeal cancer and may be a potential therapeutic target for treatment.
DOI: 10.6084/m9.figshare.21936633
title: Tracing the relationship among HIV-1 sub-subtype F1 strains: a phylodynamic perspective

description: BACKGROUND The human immunodeficiency virus type 1, F1 sub-subtype (HIV-1 F1) circulates in three continents: Africa, Europe, and South America. In Brazil, this sub-subtype co-circulates with subtypes B and C and several recombinant forms, mainly BF1 variants. OBJECTIVES This study aimed to reconstruct the dynamic history of HIV-1 F1 in Brazil. METHODS HIV-1 near full-length genome and pol gene nucleotide sequences available in public databases were assembled in two datasets (POL671 and NFLG53) to cover the largest number of F1 sub-subtype sequences. Phylodynamic and temporal analyses were performed. FINDINGS Two main strains of the F1 sub-subtype are circulating worldwide. The first (F1.I) was found among Brazilian samples (75%) and the second (F1.II) among Romanian (62%) and other European and African isolates. The F1 subtype epidemic in Brazil originated from a single entry into the country around 1970. This ancestral sample is related to samples isolated in European countries (France, Finland, and Belgium), which are possibly of African origin. Moreover, further migration (1998 CI: 1994-2003) of strains from Brazil to Europe (Spain and the UK) was observed. Interestingly, all different recombinant BF patterns found, even those from outside Brazil, present the same F1 lineage (F1.I) as an ancestor, which could be related to the acquisition of adaptive advantages for the recombinant progenies. MAIN CONCLUSIONS These findings are important for the understanding of the origin and dynamics of the F1 sub-subtype and a consequent better and greater understanding of the HIV-1 F1 and BF epidemic that still spreads from Brazil to other countries.
DOI: 10.6084/m9.figshare.21936633.v1
title: Tracing the relationship among HIV-1 sub-subtype F1 strains: a phylodynamic perspective

description: BACKGROUND The human immunodeficiency virus type 1, F1 sub-subtype (HIV-1 F1) circulates in three continents: Africa, Europe, and South America. In Brazil, this sub-subtype co-circulates with subtypes B and C and several recombinant forms, mainly BF1 variants. OBJECTIVES This study aimed to reconstruct the dynamic history of HIV-1 F1 in Brazil. METHODS HIV-1 near full-length genome and pol gene nucleotide sequences available in public databases were assembled in two datasets (POL671 and NFLG53) to cover the largest number of F1 sub-subtype sequences. Phylodynamic and temporal analyses were performed. FINDINGS Two main strains of the F1 sub-subtype are circulating worldwide. The first (F1.I) was found among Brazilian samples (75%) and the second (F1.II) among Romanian (62%) and other European and African isolates. The F1 subtype epidemic in Brazil originated from a single entry into the country around 1970. This ancestral sample is related to samples isolated in European countries (France, Finland, and Belgium), which are possibly of African origin. Moreover, further migration (1998 CI: 1994-2003) of strains from Brazil to Europe (Spain and the UK) was observed. Interestingly, all different recombinant BF patterns found, even those from outside Brazil, present the same F1 lineage (F1.I) as an ancestor, which could be related to the acquisition of adaptive advantages for the recombinant progenies. MAIN CONCLUSIONS These findings are important for the understanding of the origin and dynamics of the F1 sub-subtype and a consequent better and greater understanding of the HIV-1 F1 and BF epidemic that still spreads from Brazil to other countries.