DOI: 10.5061/dryad.kwh70rz9j
title: Data from: Male responses to sperm competition risk associated with increased macronutrient intake and reduced lifespan

description: Increased expenditure on the ejaculate is a taxonomically widespread male
 response to sperm competition. Increased ejaculate expenditure is assumed
 to come at a cost to future reproduction, otherwise males should always
 invest maximally. However, the life-history costs of strategic ejaculation
 are not well documented. Macronutrient intake is known to affect the
 trade-off between reproduction and lifespan. Intakes of protein and
 carbohydrate that maximise reproduction are often differ from those that
 maximise lifespan. Here, we asked whether strategic expenditure on the
 ejaculate by male crickets, Teleogryllus oceanicus, is mediated by
 macronutrient intake, and whether it comes at a cost of reduced lifespan.
 Males were exposed to rival song throughout their lifespan or were held in
 a silent non-competitive environment. Males exposed to song had a higher
 intake of both protein and carbohydrate, they reached adulthood sooner,
 produced ejaculates of higher quality, and died sooner than males living
 in a silent environment. Our findings provide a rare example of both the
 mechanisms and life-history costs associated with strategic ejaculation.
description: This excel file contains three data sheets with data necessary to
 reproduce the analyses and figures provided within Simmons & Chan
 2023. Sheet 1, intake summary & trait data Column - description A
 - Cricket - ID of individual cricket coded by treatment and a unique
 number (e.g. A1 or B1). A and B refers to two occasions on which 8th
 instar nymphs were drawn from the stock population.. B - Treatment - NS,
 silent or no-song environment; S, song environment C - Date eclosed to
 adult D - Death - date of death E - weight at adult eclosion in grams F -
 total amount of Carbohydrate consumed during lifetime (from sheet C
 intake) G- total amount of Protein consumed during lifetime (from sheet P
 intake) H - intake of C per gram of cricket per day of life I - intake of
 P per gram of cricket per day of life J - lifespan (days alive) K - days
 from 9th instar moult to adult moult L - spermatophore (sphor 1) on day
 14, yes=1, no=0 M - number of live sperm stained green in first
 spermatophore (blank cells are missing values because the male did not
 produce a spermatophore) N - number of dead sperm stained red in first
 spermatophore (blank cells are missing values because the male did not
 produce a spermatophore) O - spermatophore (sphor 2) on day 28, yes=1,
 no=0 P - number of live sperm stained green in second spermatophore (blank
 cells are missing values because the male did not produce a spermatophore)
 Q - number of dead sperm stained red in second spermatophore (blank cells
 are missing values because the male did not produce a spermatophore) R -
 total sperm counted in both first and second sperm sample = 500. Column
 used to calculate sperm viability SV1 and SV2 S - proportion live sperm,
 sperm viability in spermatophore 1 (day 14) T - proportion live sperm,
 sperm viability in spermatophore 2 (day 28) U - ration of Protein to
 Carbohydrate eaten over lifespan Sheets 2 and 3 The weights of
 carbohydrate (sheet C intake) and protein (sheet P intake) diet consumed
 by each cricket on each successive exchange of food dishes throughout
 lifespan. Note that cricket NSA18 has two cells of missing data (n/a in
 sheet C intake) for the first C food dish, which was dropped while being
 placed into the crickets enclosure. No spare dishes of carbohydrate were
 available, so that on the first feeding occasion this cricket was provided
 with the protein diet only. Blank cells reflect the fact that the
 individual had died and was no longer in the experiment.