DOI: 10.5061/dryad.mcvdnck7c
title: Cortical VIP neurons as a critical node for dopamine actions

description: Although dopamine modulates diverse cognitive processes of the prefrontal
 cortex including working memory, its underlying mechanisms are unclear. We
 investigated the roles of prefrontal vasoactive intestinal polypeptide
 (VIP)-expressing neurons, which are interneurons rich in D1 receptors
 (D1Rs), in working memory in mice engaged in a delayed match-to-sample
 task. VIP neurons conveyed robust working-memory signals and their
 inactivation profoundly impaired behavioral performance. Also, selective
 D1R knockdown in VIP neurons but not in pyramidal neurons impaired
 behavioral performance, indicating that VIP neurons are the primary
 mediators of dopamine effects on working memory. Additionally, we found
 that delay-period dopamine release dynamics vary depending on target
 location and that dopaminergic terminal stimulation increases prefrontal
 neuronal target selectivity in a laterality-dependent manner. These
 results indicate dopamine enhances working memory based on target
 laterality. These findings shed light on dopamine-modulated prefrontal
 neural processes underlying higher-order cognitive functions.
description: We implanted a GRIN lens with prism targeting layer 2/3 (VIP
 neuronal calcium imaging and dopamine-sensor imaging) or layer 5
 (pyramidal neuronal calcium imaging) in the mPFC (2.0 mm ventral to brain
 surface). And recorded the Calcium signals and GRAB sensor signals in the
 mPFC during the delayed match-to-sample task. 
description: # Cortical VIP neurons as a critical node for dopamine actions These
 datasets include raw calcium traces of VIP neurons and pyramidal neurons
 and raw GRAB-DA sensor traces of VIP-Cre mice in the mPFC during the
 delayed match-to-sample task. ## Description of the data and file
 structure 1. VIP recording data 1_VIP_Training, 208 cells
 1_VIP_Well-trained, 684 cells First column: Each cell calcium raw trace.
 Trials x time (refer time.mat file) Second column: choice information (1,
 right correct; -1, left correct; 0.5, right error; -0.5, left error) time,
 time 0 from delay onset (s), 4-s delay, delayed match-to-sample task. 2.
 GRAB-DA data 2_GRAB_Training, 7 mice 2_GRAB_Well-trained, 10 mice
 2_GRAB_Well-trained (15 s), 7 mice First column: Each mouse GRAB raw
 trace. Trials x time (refer time.mat file) Second column: choice
 information (1, right correct; -1, left correct; 0.5, right error; -0.5,
 left error) time, time 0 from delay onset (s), 4-s delay, delayed
 match-to-sample task. time_15s, time 0 from delay onset (s), 15-s delay,
 delayed match-to-sample task. 3. DTS data 3_DTS_VIP_4s, 427 cells
 3_DTS_PYR_4s, 3105 cells 3_DTS_VIP_15s, 174 cells 3_DTS_PYR_15s, 1579
 cells First column: Each cell calcium raw trace. Trials x time (refer
 time.mat file) Second column: choice information (1, right correct; -1,
 left correct; 0.5, right error; -0.5, left error) Third column: Light
 stimulation (1,n ON-trial; 0, OFF-trial) ``` 4-s delay session, entire 4-s
 delay was stimulated; 15-s delay session, second-half delay 7.5 s was
 stimulated. ``` time, time 0 from delay onset (s), 4-s delay, delayed
 match-to-sample task. time_15s, time 0 from delay onset (s), 15-s delay,
 delayed match-to-sample task. ## Sharing/Access information Links to other
 publicly accessible locations of the data: *
 [https://www.dropbox.com/scl/fo/4rvq5r0zehton53r3fmd0/h?rlkey=7fqn72zqnpoka5bsjnb78w9so&dl=0](https://www.dropbox.com/scl/fo/4rvq5r0zehton53r3fmd0/h?rlkey=7fqn72zqnpoka5bsjnb78w9so&dl=0)