DOI: 10.5061/dryad.dk68t
title: Data from: Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity

description: The somatosensory nervous system is critical for the organism’s ability to
 respond to mechanical, thermal, and nociceptive stimuli. Somatosensory
 neurons are functionally and anatomically diverse but their molecular
 profiles are not well-defined. Here, we used transcriptional profiling to
 analyze the detailed molecular signatures of dorsal root ganglion (DRG)
 sensory neurons. We used two mouse reporter lines and surface IB4 labeling
 to purify three major non-overlapping classes of neurons:
 1)IB4+SNS-Cre/TdTomato+, 2)IB4-SNS-Cre/TdTomato+, and 3)Parv-Cre/TdTomato+
 cells, encompassing the majority of nociceptive, pruriceptive, and
 proprioceptive neurons. These neurons displayed distinct expression
 patterns of ion channels, transcription factors, and GPCRs. Highly
 parallel qRT-PCR analysis of 334 single neurons selected by membership of
 the three populations demonstrated further diversity, with unbiased
 clustering analysis identifying six distinct subgroups. These data
 significantly increase our knowledge of the molecular identities of known
 DRG populations and uncover potentially novel subsets, revealing the
 complexity and diversity of those neurons underlying somatosensation.The
 somatosensory nervous system is critical for the organism’s ability to
 respond to mechanical, thermal, and nociceptive stimuli. Somatosensory
 neurons are functionally and anatomically diverse but their molecular
 profiles are not well-defined. Here, we used transcriptional profiling to
 analyze the detailed molecular signatures of dorsal root ganglion (DRG)
 sensory neurons. We used two mouse reporter lines and surface IB4 labeling
 to purify three major non-overlapping classes of neurons:
 1)IB4+SNS-Cre/TdTomato+, 2)IB4-SNS-Cre/TdTomato+, and 3)Parv-Cre/TdTomato+
 cells, encompassing the majority of nociceptive, pruriceptive, and
 proprioceptive neurons. These neurons displayed distinct expression
 patterns of ion channels, transcription factors, and GPCRs. Highly
 parallel qRT-PCR analysis of 334 single neurons selected by membership of
 the three populations demonstrated further diversity, with unbiased
 clustering analysis identifying six distinct subgroups. These data
 significantly increase our knowledge of the molecular identities of known
 DRG populations and uncover potentially novel subsets, revealing the
 complexity and diversity of those neurons underlying somatosensation.
description: Comparison of
 SNS-Cre/TdT vs. Parv-Cre/TdT neuron expression profilesDifferential expression
 analysis of microarray data from SNS-Cre/TdTomato+ neurons (n=4) vs.
 Parv-Cre/TdTomato+ neurons (n=4). Transcripts are ranked by fold-change,
 with the following information given: Affymetrix ID, genebank accession
 number, gene symbol, description, average RMA normalized levels, standard
 deviation, fold-change, p-value and FDR.Supplementary File
 1.xlsxComparison of IB4
 positive vs. IB4 negative SNS-Cre/TdT neuron profilesDifferential expression
 analysis of microarray data from IB4+SNS-Cre/TdTomato+ neurons (n=3) vs.
 IB4-SNS-Cre/TdTomato+ neurons (n=3). These cells were sorted from the same
 animals. Transcripts are ranked by fold-change, with the following
 information given: Affymetrix ID, genebank accession number, gene symbol,
 description, average RMA normalized levels, standard deviation,
 fold-change, p-value and FDR.Supplementary File
 2.xlsx