cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0h130rf
title: CIL:26574, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); TexasRed
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Immunofluorescence images used to investigate stabilization of microtubules (MTs) by Von Hippel-Lindau tumor suppressor protein (pVHL). VHL-deficient RCC-4 cells expressing wild-type VHL30 protein have a higher alpha-tubulin (MTs; green) signal relative to GSK-3beta (red; an internal fluorescence control), compared with RCC-4 control cells, suggesting that VHL stabilizes MTs. RCC-4 cells were infected with a retroviral vector to express pVHL30, fixed in 3% paraformaledehyde and permeabilized in 0.2% Triton X-100. Primary antibodies used were: monoclonal anti-GSK3beta and polyclonal anti-alpha tubulin. Secondary antibodies used were: anti-rabbit FITC and anti-mouse Texas-Red. Stacks of 5 z planes were acquired with a Leica SP2 system using the 63x 1.4NA, Oil, HCX Plan-Apo Objective and the distance between z planes is 1 micron. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--Fig 1C (right panels) and the images of the control are in Fig 1C (left panels) and CIL# 31263.
DOI: 10.6075/j0b56hkc
title: CIL:26571, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library

description: Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP comets throughout the cell. This video compares the number of EB3-GFP comets detected after nocodazole perturbation in an RCC-4 cell with active VHL. Compare with control video (RCC-4 alone) in CIL # 32024; there is an increased number of growing MTs when VHL is active. RCC-4 cells were infected with a retroviral vector to express EB3-GFP. Cells on round coverslips were transferred to a homemade holding device, and 600 µl of the appropriate medium, containing 40 nM nocodazole was added. The video was acquired with a microscope (IX70 Delta Vision Spectris; Olympus), temperature-controlled at 37C, using a 60× NA 1.4 differential interference contrast (DIC) oil Plan-Apochromat objective, ex 470 em 520, and a camera (CoolSNAP HQ; Roper Industries) with an exposure time of 100 ms and a frame rate of 0.5 s. Acquisition software used was SoftWoRx version 3.3.4 (Applied Precision). This is the raw image file for the right half of Video 5 in J Cell Biol. 2010. 190: 991-1003.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: widefield illumination; time lapse microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0cz3621
title: CIL:26586, Homo sapiens, cervical epithelium. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: frame averaging
Data qualification: Processed
description: HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein Snu114 and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The Snu114 is confined to the nucleoplasm. From Fig 1A in Huranova et al. 2010 J Cell Biol 191:75-86.
DOI: 10.6075/j0862fbm
title: CIL:26604, Homo sapiens, cervical epithelium. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: line averaging
Data qualification: Processed
description: HeLa cells stably expressing the BAC-encoded GFP-labeled U2B snRNP and MS2-mRED protein and treated with doxycholine to express the E3 transgene. Shown is the GFP signal. Cells imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. From Fig 5C in Huranova et al. 2010 J Cell Biol 191:75-86.
DOI: 10.6084/m9.figshare.30496796
title: <b>Nationwide larval mosquito sampling in Cambodian households: Vector species in anthropogenic breeding sites</b>

description: Vector control remains a key strategy in reducing mosquito-borne disease transmission. Understanding mosquito species distribution, diversity, and breeding habitat ecology is crucial for effective surveillance and to define targeted vector control interventions. We conducted a study to understand the diversity and habitat preferences of mosquito larvae across Cambodia. Mosquito larvae were collected from a variety of breeding habitats located around households across all 25 provinces. The national sampling was conducted once during the rainy season in urban (city) and rural (village) areas within each province. Collected larvae were reared to adult emergence in the insectarium for morphological identification, further confirmed with molecular techniques. We found 37 mosquito species in the households, of which 12 are vectors of pathogens such as dengue and Japanese encephalitis viruses, and <i>Plasmodium</i> species, representing 93% of all collected mosquitoes. Larvae were predominantly found in anthropized artificial breeding habitats, accounting for 98% of all larvae collected. Notably, the two primary dengue vectors, <i>Aedes aegypti</i> and <i>Ae. albopictus</i>, were recorded from all 52 sampling locations. In addition, our study identified the presence of <i>Aedes vittatus </i>in 12 provinces, a new confirmed vector of dengue in Cambodia. We also recorded eight Japanese encephalitis vectors, with at least one species from all sampling sites. There were no statistically significant differences in larval mosquito biodiversity (relative abundance, number of species, Shannon and Simpson diversity indices) between cities and villages, with 15 species occurring in both environments, representing 41% of the species and 99% of all mosquitoes collected. The widespread and predominance of dengue and Japanese encephalitis vectors in every household confirms the endemic circulation of these diseases in Cambodia.
DOI: 10.6084/m9.figshare.30496796.v1
title: <b>Nationwide larval mosquito sampling in Cambodian households: Vector species in anthropogenic breeding sites</b>

description: Vector control remains a key strategy in reducing mosquito-borne disease transmission. Understanding mosquito species distribution, diversity, and breeding habitat ecology is crucial for effective surveillance and to define targeted vector control interventions. We conducted a study to understand the diversity and habitat preferences of mosquito larvae across Cambodia. Mosquito larvae were collected from a variety of breeding habitats located around households across all 25 provinces. The national sampling was conducted once during the rainy season in urban (city) and rural (village) areas within each province. Collected larvae were reared to adult emergence in the insectarium for morphological identification, further confirmed with molecular techniques. We found 37 mosquito species in the households, of which 12 are vectors of pathogens such as dengue and Japanese encephalitis viruses, and <i>Plasmodium</i> species, representing 93% of all collected mosquitoes. Larvae were predominantly found in anthropized artificial breeding habitats, accounting for 98% of all larvae collected. Notably, the two primary dengue vectors, <i>Aedes aegypti</i> and <i>Ae. albopictus</i>, were recorded from all 52 sampling locations. In addition, our study identified the presence of <i>Aedes vittatus </i>in 12 provinces, a new confirmed vector of dengue in Cambodia. We also recorded eight Japanese encephalitis vectors, with at least one species from all sampling sites. There were no statistically significant differences in larval mosquito biodiversity (relative abundance, number of species, Shannon and Simpson diversity indices) between cities and villages, with 15 species occurring in both environments, representing 41% of the species and 99% of all mosquitoes collected. The widespread and predominance of dengue and Japanese encephalitis vectors in every household confirms the endemic circulation of these diseases in Cambodia.
DOI: 10.6075/j04f1pmw
title: CIL:26603, Homo sapiens, cervical epithelium. In Cell Image Library

description: HeLa cells stably expressing the BAC-encoded GFP-labeled U1-70K snRNP and MS2-mRED protein and treated with doxycholine to express the E3 transgene. Shown is the GFP signal. Cells imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. From Fig 5C in Huranova et al. 2010 J Cell Biol 191:75-86.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: line averaging
Data qualification: Processed
DOI: 10.6075/j0q23z8z
title: CIL:2664, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
DOI: 10.6075/j00p0xw5
title: CIL:26602, Homo sapiens, cervical epithelium. In Cell Image Library

description: HeLa cells stably expressing the BAC-encoded GFP-labeled U2B snRNP and Ms2-mRED protein and treated with doxycholine to express the E3 transgene. Shown is the MS2-mRED signal. Cells imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. From Fig 5C in Huranova et al. 2010 J Cell Biol 191:75-86.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; pDsRED-Monomer-N1
Processing history: frame averaging
Data qualification: Processed
DOI: 10.6075/j0tt4px2
title: CIL:2661, Caenorhabditis elegans. In Cell Image Library

description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j0cc0zxb
title: CIL:266, Mus musculus, skeletal muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: uranyl salt; lead salt
Visualization methods: detection of electrons
Processing history: unprocessed raw data
description: Thin section electron microscopy of diaphragm (skeletal) muscle from a wild type mouse.

Sample was viewed with a Hitachi 7600 electron microscope (accelerating voltage 80 KV) and imaged with an AMT digital camera. Image taken at 10,000X magnification.

This image is part of a set of wild type diaphragm muscle images that served as controls for the study of the delta-sarcoglycan knockout mouse. Both the set of wild-type and delta-sarcoglycan knockout mouse images are part of The CELL: an image library.

Reference: Nat Med. 2008 Apr;14(4):442-7. 
Genetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy.
Millay DP, Sargent MA, Osinska H, Baines CP, Barton ER, Vuagniaux G, Sweeney HL, Robbins J, Molkentin JD (tissue preparation described in Fewell JG, et al. A treadmill exercise regimen for identifying cardiovascular phenotypes in transgenic mice. Am J Physiol. 1997;273:H1595–H1605)
DOI: 10.6075/j0765d7p
title: CIL:26596, Homo sapiens, cervical epithelium. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Processed
description: HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U1-70K and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The U1-70K protein is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). From Fig 4A in Huranova et al. 2010 J Cell Biol 191:75-86.
DOI: 10.6075/j0s46qtp
title: CIL:26595, Homo sapiens, cervical epithelium. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Processed
description: HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U1-70K and treated with isoginkgetin. The protein localizes to enlarged splicing factor compartments. Cells imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. From Fig 4A in Huranova et al. 2010 J Cell Biol 191:75-86.
DOI: 10.6075/j06972mz
title: CIL:2667, Caenorhabditis elegans. In Cell Image Library

description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j0w0951f
title: CIL:2666, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j0bg2n1f
title: CIL:2665, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
DOI: 10.6075/j06d5s2r
title: CIL:2672, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
DOI: 10.6075/j03j3c11
title: CIL:2663, Caenorhabditis elegans. In Cell Image Library

description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data