DOI: 10.5061/dryad.1vn46
title: Data from: A comparative assessment of SNP and microsatellite markers for assigning parentage in a socially monogamous bird

description: Single-nucleotide polymorphisms (SNPs) are preferred over microsatellite
 markers in many evolutionary studies, but have only recently been applied
 to studies of parentage. Evaluations of SNPs and microsatellites for
 assigning parentage have mostly focused on special cases that require a
 relatively large number of heterozygous loci, such as species with low
 genetic diversity or with complex social structures. We developed 120 SNP
 markers from a transcriptome assembled using RNA-sequencing of a songbird
 with the most common avian mating system—social monogamy. We compared the
 effectiveness of 97 novel SNPs and six previously described
 microsatellites for assigning paternity in the black-throated blue
 warbler, Setophaga caerulescens. We show that the full panel of 97 SNPs
 (mean Ho = 0.19) was as powerful for assigning paternity as the panel of
 multiallelic microsatellites (mean Ho = 0.86). Paternity assignments using
 the two marker types were in agreement for 92% of the offspring. Filtering
 individual samples by a 50% call rate and SNPs by a 75% call rate
 maximized the number of offspring assigned with 95% confidence using SNPs.
 We also found that the 40 most heterozygous SNPs (mean Ho = 0.37) had
 similar power to assign paternity as the full panel of 97 SNPs. These
 findings demonstrate that a relatively small number of variable SNPs can
 be effective for parentage analyses in a socially monogamous species. We
 suggest that the development of SNP markers is advantageous for studies
 that require high-throughput genotyping or that plan to address a range of
 ecological and evolutionary questions.
description: 2011 genotypes msat
 50Microsatellite genotypes
 of offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;50%.2011 genotypes snps
 50SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;50%.2012 genotypes msats
 50Microsatellite genotypes
 of offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;50%.2012 genotypes snps
 50SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;50%.2011 genotypes snps
 75SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci call rate
 &gt;75%.2012 genotypes snps
 75SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci call rate
 &gt;75%.2011 genotypes snps
 90SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci call rate
 &gt;90%.2012 genotypes snps
 90SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci call rate
 &gt;90%.2011 genotypes ind
 75SNPs genotypes of
 offspring and adult black-throated blue warblers. Sample call rate
 &gt;75%.2012 genotypes ind
 75SNPs genotypes of
 offspring and adult black-throated blue warblers. Sample call rate
 &gt;75%.2011 genotypes ind
 90SNPs genotypes of
 offspring and adult black-throated blue warblers. Sample call rate
 &gt;90%.2012 genotypes ind
 90SNPs genotypes of
 offspring and adult black-throated blue warblers. Sample call rate
 &gt;90%.2011 genotypes ind snps
 75SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;75%.2012 genotypes ind snps
 75SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;75%.2011 genotypes ind snps
 90SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;90%.2012 genotypes ind snps
 90SNPs genotypes of
 offspring and adult black-throated blue warblers. Loci and sample call
 rate &gt;90%.2011 genotypes 77
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 77 Loci with the highest
 call rates.2012 genotypes 77
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 77 Loci with the highest
 call rates.2011 genotypes 57
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 57 Loci with the highest
 call rates.2012 genotypes 57
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 57 Loci with the highest
 call rates.2011 genotypes 37
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 37 Loci with the highest
 call rates.2012 genotypes 37
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 37 Loci with the highest
 call rates.2011 genotypes 27
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 27 Loci with the highest
 call rates.2012 genotypes 27
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 27 Loci with the highest
 call rates.2011 genotypes 17
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 17 Loci with the highest
 call rates.2012 genotypes 17
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 17 Loci with the highest
 call rates.2011 genotypes 7
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 7 Loci with the highest
 call rates.2012 genotypes 7
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 7 Loci with the highest
 call rates.2011 genotypes 77H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 77 most heterozygous
 loci.2012 genotypes 77H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 77 most heterozygous
 loci.2011 genotypes 57H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 57 most heterozygous
 loci.2012 genotypes 57H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 57 most heterozygous
 loci.2011 genotypes 37H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 37 most heterozygous
 loci.2012 genotypes 37H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 37 most heterozygous
 loci.2011 genotypes 27H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 27 most heterozygous
 loci.2012 genotypes 27H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 27 most heterozygous
 loci.2011 genotypes 17H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 17 most heterozygous
 loci.2012 genotypes 17H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 17 most heterozygous
 loci.2011 genotypes 7H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 7 most heterozygous
 loci.2012 genotypes 7H
 snpsSNPs genotypes of
 offspring and adult black-throated blue warblers. 7 most heterozygous
 loci.