cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11889951
title: Supplementary Material for: Statins Directly Regulate Pituitary Cell Function and Exert Antitumor Effects in Pituitary Tumors

description: <b><i>Introduction:</i></b> Pituitary neuroendocrine tumors (PitNETs), the most abundant of all intracranial tumors, entail severe comorbidities. First-line therapy is transsphenoidal surgery, but subsequent pharmacological therapy is often required. Unfortunately, many patients are/become unresponsive to available drugs (somatostatin analogues [SSAs]/dopamine agonists), underscoring the need for new therapies. Statins are well-known drugs commonly prescribed to treat hyperlipidemia/cardiovascular diseases, but can convey additional beneficial effects, including antitumor actions. The direct effects of statins on normal human pituitary or PitNETs are poorly known. Thus, we aimed to explore the direct effects of statins, especially simvastatin, on key functional parameters in normal and tumoral pituitary cells, and to evaluate the combined effects of simvastatin with metformin (MF) or SSAs. <b><i>Methods:</i></b> Effects of statins in cell proliferation/viability, hormone secretion, and signaling pathways were evaluated in normal pituitary cells from a primate model (<i>Papio anubis</i>), tumor cells from corticotropinomas, somatotropinomas, nonfunctioning pituitary tumors, and PitNET cell-lines (AtT20/GH3-cells). <b><i>Results:</i></b> All statins decreased AtT20-cell proliferation, simvastatin showing stronger effects. Indeed, simvastatin reduced cell viability and/or hormone secretion in all PitNETs subtypes and cell-lines, and ACTH/GH/PRL/FSH/LH secretion (but not expression), in primate cell cultures, by modulating MAPK/PI3K/mTOR pathways and expression of key receptors (GH-releasing hormone-receptor/ghrelin-R/Kiss1-R) regulating pituitary function. Addition of MF or SSAs did not enhance simvastatin antitumor effects. <b><i>Conclusion:</i></b> Our data reveal direct antitumor effects of simvastatin on PitNET-cells, paving the way to explore these compounds as a possible tool to treat PitNETs.
DOI: 10.6084/m9.figshare.11889963.v1
title: Monitoring Cocoa Expansion into Croplands in Ghana Cocoa Forest Mosaic Landscape: A Case Study of Juabeso District

description: This research provides useful insights into land use dynamics in the cocoa-forest mosaic landscape and other contexts of cocoa expansion, which are often overlooked in the policy discourse, as well as the formulation of sustainability interventions in the cocoa landscape and value chain. This study draws on remote sensing applications in crop land area mapping in cocoa landscapes to focus greater attention on the implications of cropland area loss, due to cocoa expansion. This will inform policy and interventions in the landscape to minimize possible negative effects of cropland area loss on food security and livelihoods within cocoa landscapes.<br>A survey of 407 farmers in the Juabeso District to unravel the dynamics, reasons and complexities of croplands conversion to cocoa and its implications on food security. Semi-structured questionnaires were used for the survey. The communities selected were - Juabeso Nkwanta, Kwaokrom, Antobia, Adiembra, Helehele, Gyato, Eluibo, Abono, Mafia, Abrokofe, Brekrom, Boinzan, Seniagyokrom, and Adwumam.
DOI: 10.6084/m9.figshare.11889951.v1
title: Supplementary Material for: Statins Directly Regulate Pituitary Cell Function and Exert Antitumor Effects in Pituitary Tumors

description: <b><i>Introduction:</i></b> Pituitary neuroendocrine tumors (PitNETs), the most abundant of all intracranial tumors, entail severe comorbidities. First-line therapy is transsphenoidal surgery, but subsequent pharmacological therapy is often required. Unfortunately, many patients are/become unresponsive to available drugs (somatostatin analogues [SSAs]/dopamine agonists), underscoring the need for new therapies. Statins are well-known drugs commonly prescribed to treat hyperlipidemia/cardiovascular diseases, but can convey additional beneficial effects, including antitumor actions. The direct effects of statins on normal human pituitary or PitNETs are poorly known. Thus, we aimed to explore the direct effects of statins, especially simvastatin, on key functional parameters in normal and tumoral pituitary cells, and to evaluate the combined effects of simvastatin with metformin (MF) or SSAs. <b><i>Methods:</i></b> Effects of statins in cell proliferation/viability, hormone secretion, and signaling pathways were evaluated in normal pituitary cells from a primate model (<i>Papio anubis</i>), tumor cells from corticotropinomas, somatotropinomas, nonfunctioning pituitary tumors, and PitNET cell-lines (AtT20/GH3-cells). <b><i>Results:</i></b> All statins decreased AtT20-cell proliferation, simvastatin showing stronger effects. Indeed, simvastatin reduced cell viability and/or hormone secretion in all PitNETs subtypes and cell-lines, and ACTH/GH/PRL/FSH/LH secretion (but not expression), in primate cell cultures, by modulating MAPK/PI3K/mTOR pathways and expression of key receptors (GH-releasing hormone-receptor/ghrelin-R/Kiss1-R) regulating pituitary function. Addition of MF or SSAs did not enhance simvastatin antitumor effects. <b><i>Conclusion:</i></b> Our data reveal direct antitumor effects of simvastatin on PitNET-cells, paving the way to explore these compounds as a possible tool to treat PitNETs.
DOI: 10.6084/m9.figshare.11889933
title: Produção tecnológica

description: Esse conjunto de dados refere-se a produção de aplicativos, patentes e produtos desenvolvidos pelos programas de pós-graduação brasileiros no período de 2013-2016. Esses datasets foram utilizados para cálculo do índice de produção tecnológica - IPT <br>
DOI: 10.6084/m9.figshare.11889942
title: Resultado do IPT

description: Este <i>Dataset</i> refere-se ao cálculo de Índice de Produção Tecnológica (IPT) da tese de Vívian Costa Alves <br>
DOI: 10.6084/m9.figshare.11889933.v1
title: Produção tecnológica

description: Esse conjunto de dados refere-se a produção de aplicativos, patentes e produtos desenvolvidos pelos programas de pós-graduação brasileiros no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889942.v1
title: Resultado do IPT

description: Este <i>Dataset</i> refere-se ao cálculo de Índice de Produção Tecnológica (IPT) da tese de Vívian Costa Alves <br>
DOI: 10.6084/m9.figshare.11889927
title: Membros de projetos

description: Este dataset refere-se aos membros dos projetos executados no período de 2013-2016 pelos programas de pós-graduação brasileiros.
DOI: 10.6084/m9.figshare.11889924
title: Differential gene expression profiling of the goose pineal gland

description: 1. The present study was conducted to obtain a better understanding of the molecular mechanisms underlying broodiness in a commercial breed, Tianfu geese, as little is known about the role of the pineal gland in this period. The aim was to identify genes which are differentially expressed in the pineal gland between the laying and broodiness periods by performing a transcriptome screen. 2. After sequencing <i>cDNA</i> derived from the pineal gland and annotation of the results, a sequencing depth of 14.82 and 18.17 million mapped tags was obtained during the laying and broodiness periods, respectively, and a total of 120 differentially expressed genes were identified. Of these, 32 genes showing up-regulated expression and 88 genes showing down-regulated expression were identified in broodiness period <i>vs</i>. laying period libraries. 3. Gene ontology (GO) analyses showed that these genes were related to the visual process, phototransduction, and lipoprotein metabolism. Kyoto Encyclopaedia of Genes and Genome (KEGG) analyses showed that phototransduction and tryptophan metabolism pathways exhibited the largest enrichment factors. The reliability of the RNA sequence data was confirmed by quantitative real-time PCR analysis of five genes, and the results were mostly consistent with those from the high-throughput RNA sequencing. 4. The goose transcriptome and the identification of differentially expressed genes provided comprehensive gene expression information that enables a better understanding of the molecular mechanisms underlying the broodiness period of geese.
DOI: 10.6084/m9.figshare.11889924.v1
title: Differential gene expression profiling of the goose pineal gland

description: 1. The present study was conducted to obtain a better understanding of the molecular mechanisms underlying broodiness in a commercial breed, Tianfu geese, as little is known about the role of the pineal gland in this period. The aim was to identify genes which are differentially expressed in the pineal gland between the laying and broodiness periods by performing a transcriptome screen. 2. After sequencing <i>cDNA</i> derived from the pineal gland and annotation of the results, a sequencing depth of 14.82 and 18.17 million mapped tags was obtained during the laying and broodiness periods, respectively, and a total of 120 differentially expressed genes were identified. Of these, 32 genes showing up-regulated expression and 88 genes showing down-regulated expression were identified in broodiness period <i>vs</i>. laying period libraries. 3. Gene ontology (GO) analyses showed that these genes were related to the visual process, phototransduction, and lipoprotein metabolism. Kyoto Encyclopaedia of Genes and Genome (KEGG) analyses showed that phototransduction and tryptophan metabolism pathways exhibited the largest enrichment factors. The reliability of the RNA sequence data was confirmed by quantitative real-time PCR analysis of five genes, and the results were mostly consistent with those from the high-throughput RNA sequencing. 4. The goose transcriptome and the identification of differentially expressed genes provided comprehensive gene expression information that enables a better understanding of the molecular mechanisms underlying the broodiness period of geese.
DOI: 10.6084/m9.figshare.11889927.v1
title: Membros de projetos

description: Este dataset refere-se aos membros dos projetos executados no período de 2013-2016 pelos programas de pós-graduação brasileiros.
DOI: 10.6084/m9.figshare.11889909
title: Phylogenetic relationships and palaeobiology of a new xenungulate (Mammalia: Eutheria) from the Palaeogene of Argentina

description: Xenungulates are a peculiar group of bilophodont ungulates, widespread over South America during the Paleocene and early Eocene. Here we describe, illustrate and analyse a new Xenungulata, <i>Rodcania kakan</i> gen. et sp. nov., the first from the lower latitudes of Argentina. It was recovered from the Río Loro Formation (Tucumán Province), for which a Paleocene age was estimated. The material comprises a fragment of the left dentary with an incomplete m2, eroded over the enamel surface, and an almost complete m3. Both teeth share with Xenungulata strong enamel, vertically oriented Hunter–Schreger bands (HSB), and wear facets sloping distally. <i>Rodcania kakan</i> differs from other xenungulates in the m3 trigonid, simplified and mesiodistally short; absence of a paraconid; more oblique protolophid in relation to the mesiodistal axis and a straight cristid obliqua, pointing to the protoconid position; and has a wider and longer talonid. A phylogenetic analysis of 13 species and 36 craniodental characters recovered <i>R. kakan</i> as a basal member of the Carodniidae, supported by 10 synapomorphies in the four most parsimonious trees. The body mass estimation of the Río Loro Fauna and of the rest of the xenungulate taxa revealed that <i>R. kakan</i> is the largest mammal of the Río Loro assemblage, weighing around 165 kg and with a size comparable to <i>Carodnia feruglioi</i>. The increase in body mass showed no clear relation with evolutionary trends in xenungulates. http://zoobank.org/urn:lsid:zoobank.org:pub:FFCB8A36-1CE1-4D5F-90D7-A5D773CF121B
DOI: 10.6084/m9.figshare.11889909.v1
title: Phylogenetic relationships and palaeobiology of a new xenungulate (Mammalia: Eutheria) from the Palaeogene of Argentina

description: Xenungulates are a peculiar group of bilophodont ungulates, widespread over South America during the Paleocene and early Eocene. Here we describe, illustrate and analyse a new Xenungulata, <i>Rodcania kakan</i> gen. et sp. nov., the first from the lower latitudes of Argentina. It was recovered from the Río Loro Formation (Tucumán Province), for which a Paleocene age was estimated. The material comprises a fragment of the left dentary with an incomplete m2, eroded over the enamel surface, and an almost complete m3. Both teeth share with Xenungulata strong enamel, vertically oriented Hunter–Schreger bands (HSB), and wear facets sloping distally. <i>Rodcania kakan</i> differs from other xenungulates in the m3 trigonid, simplified and mesiodistally short; absence of a paraconid; more oblique protolophid in relation to the mesiodistal axis and a straight cristid obliqua, pointing to the protoconid position; and has a wider and longer talonid. A phylogenetic analysis of 13 species and 36 craniodental characters recovered <i>R. kakan</i> as a basal member of the Carodniidae, supported by 10 synapomorphies in the four most parsimonious trees. The body mass estimation of the Río Loro Fauna and of the rest of the xenungulate taxa revealed that <i>R. kakan</i> is the largest mammal of the Río Loro assemblage, weighing around 165 kg and with a size comparable to <i>Carodnia feruglioi</i>. The increase in body mass showed no clear relation with evolutionary trends in xenungulates. http://zoobank.org/urn:lsid:zoobank.org:pub:FFCB8A36-1CE1-4D5F-90D7-A5D773CF121B
DOI: 10.6084/m9.figshare.11889903
title: Formação docentes

description: O conjunto de dados refere-se a formação dos docentes permanentes vinculados aos programas de pós-graduação brasileiros no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889903.v1
title: Formação docentes

description: O conjunto de dados refere-se a formação dos docentes permanentes vinculados aos programas de pós-graduação brasileiros no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889897
title: Cranial and postcranial remains of a new species of <i>Prochelidella</i> (Testudines: Pleurodira: Chelidae) from ‘La Buitrera’ (Cenomanian of Patagonia, Argentina), with comments on the monophyly of this extinct chelid genus from southern Gondwana

description: The genus <i>Prochelidella</i> is the oldest known panchelid taxon. It is widely distributed in several Cretaceous basins throughout Patagonia, Argentina. It was previously known from three named species. A fourth species presented herein, <i>Prochelidella buitreraensis</i> sp. nov., preserves both cranial and postcranial material from the Candeleros Formation (La Buitrera Area) of Río Negro Province, Argentina. Features observed in the nuchal bone (i.e. wide, trapezoidal cervical vertebrae, shallow nuchal notch) allow us to assign this specimen to the genus <i>Prochelidella</i>. Several specific traits (i.e. a large open retropterygoideum foramen, and a strongly developed muscle attachment site on the basisphenoid) suggest that the form from the Candeleros Formation is a new species of the genus <i>Prochelidella</i>. A phylogenetic analysis that includes the three best-preserved species of <i>Prochelidella</i> recovers this taxon as monophyletic. The Cenomanian age of this new taxon allows us to compare the panchelid turtles from the Patagonian (Neuquen) and Australian (Surat) basins. http://zoobank.org/urn:lsid:zoobank.org:pub:E9D194C7-126D-46B6-BF75-BD1A51A74971
DOI: 10.6084/m9.figshare.11889897.v1
title: Cranial and postcranial remains of a new species of <i>Prochelidella</i> (Testudines: Pleurodira: Chelidae) from ‘La Buitrera’ (Cenomanian of Patagonia, Argentina), with comments on the monophyly of this extinct chelid genus from southern Gondwana

description: The genus <i>Prochelidella</i> is the oldest known panchelid taxon. It is widely distributed in several Cretaceous basins throughout Patagonia, Argentina. It was previously known from three named species. A fourth species presented herein, <i>Prochelidella buitreraensis</i> sp. nov., preserves both cranial and postcranial material from the Candeleros Formation (La Buitrera Area) of Río Negro Province, Argentina. Features observed in the nuchal bone (i.e. wide, trapezoidal cervical vertebrae, shallow nuchal notch) allow us to assign this specimen to the genus <i>Prochelidella</i>. Several specific traits (i.e. a large open retropterygoideum foramen, and a strongly developed muscle attachment site on the basisphenoid) suggest that the form from the Candeleros Formation is a new species of the genus <i>Prochelidella</i>. A phylogenetic analysis that includes the three best-preserved species of <i>Prochelidella</i> recovers this taxon as monophyletic. The Cenomanian age of this new taxon allows us to compare the panchelid turtles from the Patagonian (Neuquen) and Australian (Surat) basins. http://zoobank.org/urn:lsid:zoobank.org:pub:E9D194C7-126D-46B6-BF75-BD1A51A74971
DOI: 10.6084/m9.figshare.11889846
title: Financiadores dos projetos

description: Esse conjunto de dados refere-se aos financiadores de projetos apoiados no âmbito da pós-graduação brasileira no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889855
title: Formação discente

description: O conjunto de dados apresenta a formação dos discentes matriculados nos programas de pós-graduação brasileiros no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889846.v1
title: Financiadores dos projetos

description: Esse conjunto de dados refere-se aos financiadores de projetos apoiados no âmbito da pós-graduação brasileira no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889855.v1
title: Formação discente

description: O conjunto de dados apresenta a formação dos discentes matriculados nos programas de pós-graduação brasileiros no período de 2013-2016.
DOI: 10.6084/m9.figshare.11889843
title: Supplementary Material for: Kisspeptin Neuron-Specific and Self-Sustained Calcium Oscillation in the Hypothalamic Arcuate Nucleus of Neonatal Mice: Regulatory Factors of its Synchronization

description: <b><i>Introduction:</i></b> Synchronous and pulsatile neural activation of kisspeptin neurons in the arcuate nucleus (ARN) are important components of the gonadotropin-releasing hormone pulse generator, the final common pathway for central regulation of mammalian reproduction. However, whether ARN kisspeptin neurons can intrinsically generate self-sustained synchronous oscillations from the early neonatal period and how they are regulated remain unclear. <b><i>Objective:</i></b> This study aimed to examine the endogenous rhythmicity of ARN kisspeptin neurons and its neural regulation using a neonatal organotypic slice culture model. <b><i>Methods:</i></b> We monitored calcium (Ca<sup>2+</sup>) dynamics in real-time from individual ARN kisspeptin neurons in neonatal organotypic explant cultures of <i>Kiss1</i>-IRES-Cre mice transduced with genetically encoded Ca<sup>2+</sup> indicators. Pharmacological approaches were employed to determine the regulations of kisspeptin neuron-specific Ca<sup>2+</sup> oscillations. A chemogenetic approach was utilized to assess the contribution of ARN kisspeptin neurons to the population dynamics. <b><i>Results:</i></b> ARN kisspeptin neurons in neonatal organotypic cultures exhibited a robust synchronized Ca<sup>2+</sup> oscillation with a period of approximately 3 min. Kisspeptin neuron-specific Ca<sup>2+</sup> oscillations were dependent on voltage-gated sodium channels and regulated by endoplasmic reticulum-dependent Ca<sup>2+</sup> homeostasis. Chemogenetic inhibition of kisspeptin neurons abolished synchronous Ca<sup>2+</sup> oscillations, but the autocrine actions of the neuropeptides were marginally effective. Finally, neonatal ARN kisspeptin neurons were regulated by N-methyl-D-aspartate and gamma-aminobutyric acid receptor-mediated neurotransmission. <b><i>Conclusion:</i></b> These data demonstrate that ARN kisspeptin neurons in organotypic cultures can generate synchronized and self-sustained Ca<sup>2+</sup> oscillations. These oscillations controlled by multiple regulators within the ARN are a novel ultradian rhythm generator that is active during the early neonatal period.
DOI: 10.6084/m9.figshare.11889825
title: Áreas de Atuação profissional - Docentes

description: Conjunto de dados referente a atuação profissional dos docentes dos PPG brasileiros, extraídos da plataforma Lattes.
DOI: 10.6084/m9.figshare.11889843.v1
title: Supplementary Material for: Kisspeptin Neuron-Specific and Self-Sustained Calcium Oscillation in the Hypothalamic Arcuate Nucleus of Neonatal Mice: Regulatory Factors of its Synchronization

description: <b><i>Introduction:</i></b> Synchronous and pulsatile neural activation of kisspeptin neurons in the arcuate nucleus (ARN) are important components of the gonadotropin-releasing hormone pulse generator, the final common pathway for central regulation of mammalian reproduction. However, whether ARN kisspeptin neurons can intrinsically generate self-sustained synchronous oscillations from the early neonatal period and how they are regulated remain unclear. <b><i>Objective:</i></b> This study aimed to examine the endogenous rhythmicity of ARN kisspeptin neurons and its neural regulation using a neonatal organotypic slice culture model. <b><i>Methods:</i></b> We monitored calcium (Ca<sup>2+</sup>) dynamics in real-time from individual ARN kisspeptin neurons in neonatal organotypic explant cultures of <i>Kiss1</i>-IRES-Cre mice transduced with genetically encoded Ca<sup>2+</sup> indicators. Pharmacological approaches were employed to determine the regulations of kisspeptin neuron-specific Ca<sup>2+</sup> oscillations. A chemogenetic approach was utilized to assess the contribution of ARN kisspeptin neurons to the population dynamics. <b><i>Results:</i></b> ARN kisspeptin neurons in neonatal organotypic cultures exhibited a robust synchronized Ca<sup>2+</sup> oscillation with a period of approximately 3 min. Kisspeptin neuron-specific Ca<sup>2+</sup> oscillations were dependent on voltage-gated sodium channels and regulated by endoplasmic reticulum-dependent Ca<sup>2+</sup> homeostasis. Chemogenetic inhibition of kisspeptin neurons abolished synchronous Ca<sup>2+</sup> oscillations, but the autocrine actions of the neuropeptides were marginally effective. Finally, neonatal ARN kisspeptin neurons were regulated by N-methyl-D-aspartate and gamma-aminobutyric acid receptor-mediated neurotransmission. <b><i>Conclusion:</i></b> These data demonstrate that ARN kisspeptin neurons in organotypic cultures can generate synchronized and self-sustained Ca<sup>2+</sup> oscillations. These oscillations controlled by multiple regulators within the ARN are a novel ultradian rhythm generator that is active during the early neonatal period.