DOI: 10.5061/dryad.hmgqnk9wv
title: Data from: Developmental temperature, not inbreeding, shapes life history and locomotor behaviors in juvenile guppies (Poecilia reticulata)

description: Inbreeding is thought to play a strong role in shaping life-history traits
 and behaviors.  Supporting evidence for this role often comes
 from observational studies. Experiments that establish causality and
 formally test how environmental factors moderate any effects of inbreeding
 remain underutilized. We ran an experiment to test how developmental
 temperature and inbreeding influence key life-history traits (growth rate,
 size, and age at maturity, survival, sex ratio), and locomotor behaviors
 (boldness and habituation) in juvenile guppies (Poecilia reticulata). We
 used a controlled breeding design to generate inbred and outbred
 individuals that were then reared under a control (26°C) or an elevated
 temperature (30°C) until maturity. Developmental temperature strongly
 affected life-history traits; both sexes matured earlier at 30°C, but only
 males exhibited slower early growth and reduced size at maturity. Female
 growth and size at maturity were unaffected. The higher developmental
 temperature reduced boldness in both sexes; however, only females
 habituated to the novel test environment, but this trend was only
 significant for females that developed at 26°C. In contrast, inbreeding
 had no significant effects on any of the measured traits, nor did it
 significantly interact with temperature. In sum, under our experimental
 conditions, developmental temperature is the primary driver of phenotypic
 plasticity in guppies, generating sex-specific responses in both life
 history traits and behavior, while inbreeding between siblings had no
 detectable effects on any of the measured traits, even under thermal
 stress. Our findings highlight the key role of temperature in shaping
 developmental and behavioral trajectories and reveal that a single
 generation of inbreeding may not always affect life history traits, even
 under environmental stress.
description: # Developmental temperature, not inbreeding, shapes life history and
 locomotor behaviors in juvenile guppies (Poecilia reticulata) **File
 name:** **life-history-data-chp2remm.csv** * baby_id = ID of individual
 offspring - dob = date of birth of individual offspring * father.id = id
 of father - mother.id = id of mother * crosstype = type of crossing -
 breeding = type of breeding * block = number of block - temp = temperature
 * gestation = days of gestation period - survival.maturity = if survived
 till maturity (1 = yes, 0 = no) * sex = male or female - date_maturity =
 day when one matured * age_at_maturity = number of days one took to mature
 - size_maturity_cm = size at maturity in centimetre * size_maturity_mm =
 size at maturity in millimetre - size0_cm = size at birth (cm) * size0_mm
 = size at birth (mm) - size28_cm = size at day 28 (cm) * size28_mm = size
 at day 28 (mm) - growth_rate_lncm/d = instantaneous growth rate Lncm per
 day * growth_rate_lnmm/d = instantaneous growth rate Lnmm per day -
 growth_rate_28tillmaturity = growth rate from day 28 till maturity **File
 name:** **final_locomotion_data.csv** * baby_id = id of individual
 offspring - habituation_rate = rate of habituation (slope) * boldness_rate
 = rate of boldness (slope) - breed = type of breeding * block = number of
 blocks - treatment = T is 30C, C is 26C * sex = male or female - mother_id
 = ID of mother * body_size_mm = size on the day behaviour measured (mm) -
 age_at_maturity = number of daysit took onek to mature * size_maturity_mm
 = size at maturity in millimeters - growth_rate = instantaneous growth
 rate * inzone_duration (sec)= total duration (in seconds) an individual
 stayed ithe n central open zone as a measure of his/her boldness **File
 name: Locomotor_behavior_videos.zip** Uploaded all the recorded videos of
 individualswhoe were assessed for tracking behaviours **File name:
 New_born_fish_photos.zip** Uploaded all thenewbornn fish photos (day 0),
 those were used to calculate growth. NB: There are repeated photos of the
 same individual. We measured the best one from a visual perspective. The
 ID of individual is seen in the photo (i.e. baby 5 or baby 1705, etc).
 **File name: Four_week_old_fish_photos.zip** Uploaded all the four week
 old fish photos (day 28) those were used to calculate growth. NB: there
 are repeated photos of same individual. We measured the best one from
 visual perspective. The id of individual is seen in the photo (i.e. baby 5
 or baby 1705, etc). **File name: Matured_fish_photos.zip** Uploaded all
 the matured fish photos (the day they matured) those were used measure the
 size at maturity. NB: there are repeated photos of same individual. We
 measured the best one from visual perspective. The id of individual is
 seen in the photo (i.e. baby 5 or baby 1705, etc). #### Code/Software
 **File name:** **R_code.txt** This analysis investigates how developmental
 temperature and inbreeding affect juvenile guppy growth rates. Using
 linear mixed-effects models (lmer), with random effects for mother and
 block, the study finds that temperature—more than inbreeding—shapes growth
 patterns, with further sex-specific analyses confirming notable
 temperature effects. Model assumptions were assessed using residual
 diagnostics and fit comparisons.