cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1267559.v2
title: Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, <i>Eisenia andrei</i>, using expressed sequence tags

description: The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. To gain insight into the expression profiles of coelomic cells of the earthworm, <i>Eisenia andrei</i>, we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs. From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of <i>E. andrei</i> were upregulated after the injection of <i>Bacillus subtilis</i> bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.
DOI: 10.6084/m9.figshare.1267559.v4
title: Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, <i>Eisenia andrei</i>, using expressed sequence tags

description: The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. To gain insight into the expression profiles of coelomic cells of the earthworm, <i>Eisenia andrei</i>, we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs. From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of <i>E. andrei</i> were upregulated after the injection of <i>Bacillus subtilis</i> bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.
DOI: 10.6084/m9.figshare.1267559.v3
title: Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, <i>Eisenia andrei</i>, using expressed sequence tags

description: The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. To gain insight into the expression profiles of coelomic cells of the earthworm, <i>Eisenia andrei</i>, we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs. From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of <i>E. andrei</i> were upregulated after the injection of <i>Bacillus subtilis</i> bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.
DOI: 10.6084/m9.figshare.1279394.v226
title: Early ovariectomy reveals the germline encoding of the “natural” mammalian anti-A-reactive IgM reflecting developmental malignancy.

description: The germline encoding of a non-immune immunoglobulin M (IgM) molecule in mammals was experimentally documented for the first time as a result of the specifically timed ovariectomy of C57BL/10 mice. The target of this innate antibody involves a trans-species developmental antigen that signifies malignancy when expressed in any non-developmental tissue. Although ovariectomy and castration result in uncontrolled and/or enhanced humoral and cellular immunity involving increased weights of the spleen and thymus with pronounced B and T cell production, the development of mercaptoethanol-sensitive, complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after an ovariectomy that was performed in C57BL/10 mice prior to the onset of puberty. This non-immune murine anti-A, which is complementary to the trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from the cross-reactive adaptive anti-A antibody, was strongly downregulated or did not appear in plasma of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All of the murine tissues expressed the species-specific Forssman<i> </i>reactivity, and further A-like structures were identified in the male and female reproductive and endodermal organs by reaction with innate human anti-A antibody, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by reactivity to the lectin from <i>Dolichos biflorus</i> and the agglutinin from <i>Helix pomatia </i>indicating the functional involvement of the <i>O</i>-GalNAc-determined mucin-type A-like, Tn and T (Thomsen-Friedenreich) epitopes that signify malignancy when accumulated and expressed in non-developmental tissues.
DOI: 10.6084/m9.figshare.1267558.v11
title: Cell 1

description: This is the raw image data from simultaneous Traction Force Microscopy and Particle Image Velocimetry experiments performed on migrating Physarum plasmodia
DOI: 10.6084/m9.figshare.1279394.v227
title: Early ovariectomy reveals the germline encoding of the “natural” mammalian anti-A-reactive IgM reflecting developmental malignancy.

description: The germline encoding of a non-immune immunoglobulin M (IgM) molecule in mammals was experimentally documented for the first time as a result of the specifically timed ovariectomy of C57BL/10 mice. The target of this innate antibody involves a trans-species developmental antigen that signifies malignancy when expressed in any non-developmental tissue. Although ovariectomy and castration result in uncontrolled and/or enhanced humoral and cellular immunity involving increased weights of the spleen and thymus with pronounced B and T cell production, the development of mercaptoethanol-sensitive, complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after an ovariectomy that was performed in C57BL/10 mice prior to the onset of puberty. This non-immune murine anti-A, which is complementary to the trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from the cross-reactive adaptive anti-A antibody, was strongly downregulated or did not appear in plasma of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All of the murine tissues expressed the species-specific Forssman<i> </i>reactivity, and further A-like structures were identified in the male and female reproductive and endodermal organs by reaction with innate human anti-A antibody, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by reactivity to the lectin from <i>Dolichos biflorus</i> and the agglutinin from <i>Helix pomatia </i>indicating the functional involvement of the <i>O</i>-GalNAc-determined mucin-type A-like, Tn and T (Thomsen-Friedenreich) epitopes that signify malignancy when accumulated and expressed in non-developmental tissues.
DOI: 10.6084/m9.figshare.1267543
title: Ovoviviparity in <i>Macrocheles glaber</i> (Müller) (Acari: Macrochelidae), with notes on parental care and egg cannibalism

description: In <i>Macrocheles glaber</i> (Müller), oviposition can involve either oviparity or egg production followed by immediate hatching of the larva, a process that may be called ovoviviparity. We observed three phases in the process of ovoviviparity: preoviposition behaviour, oviposition followed by holding the egg under the gnathosoma and hatching of the larva assisted by the female. Two factors appear to favour the occurrence of ovoviviparity in this species: poor feeding and poor substrate quality. We witnessed egg cannibalism four times in two of the four females that we observed. We discuss the consequences of variable oviposition strategy, parental care and egg cannibalism in <i>M. glaber</i> in the context of maximization of reproductive success.
DOI: 10.6084/m9.figshare.1267558.v12
title: Cell 1

description: This is the raw image data from simultaneous Traction Force Microscopy and Particle Image Velocimetry experiments performed on migrating Physarum plasmodia
DOI: 10.6084/m9.figshare.1267542
title: Pooling ANOVA Results from Multiply Imputed Datasets - Supplemental Materials - Example Dataset

description: Example dataset for the analysis example provided in the online supplemental material of the corresponding article.
DOI: 10.6084/m9.figshare.1279394.v228
title: Early ovariectomy reveals the germline encoding of the “natural” mammalian anti-A-reactive IgM reflecting developmental malignancy.

description: The germline encoding of a non-immune immunoglobulin M (IgM) molecule in mammals was experimentally documented for the first time as a result of the specifically timed ovariectomy of C57BL/10 mice. The target of this innate antibody involves a trans-species developmental antigen that signifies malignancy when expressed in any non-developmental tissue. Although ovariectomy and castration result in uncontrolled and/or enhanced humoral and cellular immunity involving increased weights of the spleen and thymus with pronounced B and T cell production, the development of mercaptoethanol-sensitive, complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after an ovariectomy that was performed in C57BL/10 mice prior to the onset of puberty. This non-immune murine anti-A, which is complementary to the trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from the cross-reactive adaptive anti-A antibody, was strongly downregulated or did not appear in plasma of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All of the murine tissues expressed the species-specific Forssman<i> </i>reactivity, and further A-like structures were identified in the male and female reproductive and endodermal organs by reaction with innate human anti-A antibody, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by reactivity to the lectin from <i>Dolichos biflorus</i> and the agglutinin from <i>Helix pomatia </i>indicating the functional involvement of the <i>O</i>-GalNAc-determined mucin-type A-like, Tn and T (Thomsen-Friedenreich) epitopes that signify malignancy when accumulated and expressed in non-developmental tissues.
DOI: 10.6084/m9.figshare.1267542.v1
title: Pooling ANOVA Results from Multiply Imputed Datasets - Supplemental Materials - Example Dataset

description: Example dataset for the analysis example provided in the online supplemental material of the corresponding article.
DOI: 10.6084/m9.figshare.1267543.v2
title: Ovoviviparity in <i>Macrocheles glaber</i> (Müller) (Acari: Macrochelidae), with notes on parental care and egg cannibalism

description: In <i>Macrocheles glaber</i> (Müller), oviposition can involve either oviparity or egg production followed by immediate hatching of the larva, a process that may be called ovoviviparity. We observed three phases in the process of ovoviviparity: preoviposition behaviour, oviposition followed by holding the egg under the gnathosoma and hatching of the larva assisted by the female. Two factors appear to favour the occurrence of ovoviviparity in this species: poor feeding and poor substrate quality. We witnessed egg cannibalism four times in two of the four females that we observed. We discuss the consequences of variable oviposition strategy, parental care and egg cannibalism in <i>M. glaber</i> in the context of maximization of reproductive success.
DOI: 10.6084/m9.figshare.1267543.v1
title: Ovoviviparity in <i>Macrocheles glaber</i> (Müller) (Acari: Macrochelidae), with notes on parental care and egg cannibalism

description: In <i>Macrocheles glaber</i> (Müller), oviposition can involve either oviparity or egg production followed by immediate hatching of the larva, a process that may be called ovoviviparity. We observed three phases in the process of ovoviviparity: preoviposition behaviour, oviposition followed by holding the egg under the gnathosoma and hatching of the larva assisted by the female. Two factors appear to favour the occurrence of ovoviviparity in this species: poor feeding and poor substrate quality. We witnessed egg cannibalism four times in two of the four females that we observed. We discuss the consequences of variable oviposition strategy, parental care and egg cannibalism in <i>M. glaber</i> in the context of maximization of reproductive success.
DOI: 10.6084/m9.figshare.1267558.v13
title: Cell 1

description: This is the raw image data from simultaneous Traction Force Microscopy and Particle Image Velocimetry experiments performed on migrating Physarum plasmodia
DOI: 10.6084/m9.figshare.1279394.v229
title: Early ovariectomy reveals the germline encoding of the “natural” mammalian anti-A-reactive IgM reflecting developmental malignancy.

description: The germline encoding of a non-immune immunoglobulin M (IgM) molecule in mammals was experimentally documented for the first time as a result of the specifically timed ovariectomy of C57BL/10 mice. The target of this innate antibody involves a trans-species developmental antigen that signifies malignancy when expressed in any non-developmental tissue. Although ovariectomy and castration result in uncontrolled and/or enhanced humoral and cellular immunity involving increased weights of the spleen and thymus with pronounced B and T cell production, the development of mercaptoethanol-sensitive, complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after an ovariectomy that was performed in C57BL/10 mice prior to the onset of puberty. This non-immune murine anti-A, which is complementary to the trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from the cross-reactive adaptive anti-A antibody, was strongly downregulated or did not appear in plasma of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All of the murine tissues expressed the species-specific Forssman<i> </i>reactivity, and further A-like structures were identified in the male and female reproductive and endodermal organs by reaction with innate human anti-A antibody, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by reactivity to the lectin from <i>Dolichos biflorus</i> and the agglutinin from <i>Helix pomatia </i>indicating the functional involvement of the <i>O</i>-GalNAc-determined mucin-type A-like, Tn and T (Thomsen-Friedenreich) epitopes that signify malignancy when accumulated and expressed in non-developmental tissues.
DOI: 10.6084/m9.figshare.1267541
title: New geological and geochronological data of the Placer de Guadalupe uplift, Mexico: a new piece of the Late Triassic–Jurassic Nazas Arc?

description: Basement exposed in the Placer de Guadalupe–Plomosas uplift in northern Mexico provides important clues for the geologic evolution of the region. The stratigraphic units form stacked thrust sheets of psammitic and calcareous formations, interlayered with magmatic rock. The eastern calcareous and quartzite formations exhibit structures associated with ductile deformation, whereas the upper stratigraphic units only contain structures formed via younger brittle deformation. Porphyry interlayered in the upper Plomosas Formation has a U-Pb zircon age of 171 ± 1 Ma. This age is consistent with its stratigraphic position, interbedded quartzarenites with a maximum depositional age of ~168 Ma. Granite flakes within the Horquilla Formation are dated at 209 ± 3 Ma, and the La Viñata quartzite exhibits a maximum age of ~193 Ma. The Upper Plomosas Formation correlates well with the arc-related Middle Jurassic Nazas Formation of northeastern Mexico, constituting the first report of a Jurassic continental margin arc outcrop in the ‘Central Mexican Gap zone’. We document Late Norian to Bajocian ages for the stratigraphic units cropping out in the Placer de Guadalupe area. The Jurassic age cluster indicates that the Nazas Arc magmatism in the region occurred during the Late Triassic and ended in the Middle Jurassic times. Permian ages previously assigned to these rocks and the occurrence of a Permo–Triassic deformation event have to be dismissed.
DOI: 10.6084/m9.figshare.1267558.v14
title: Cell 1

description: This is the raw image data from simultaneous Traction Force Microscopy and Particle Image Velocimetry experiments performed on migrating Physarum plasmodia
DOI: 10.6084/m9.figshare.1279394.v230
title: Early ovariectomy reveals the germline encoding of the “natural” mammalian anti-A-reactive IgM reflecting developmental malignancy.

description: The germline encoding of a non-immune immunoglobulin M (IgM) molecule in mammals was experimentally documented for the first time as a result of the specifically timed ovariectomy of C57BL/10 mice. The target of this innate antibody involves a trans-species developmental antigen that signifies malignancy when expressed in any non-developmental tissue. Although ovariectomy and castration result in uncontrolled and/or enhanced humoral and cellular immunity involving increased weights of the spleen and thymus with pronounced B and T cell production, the development of mercaptoethanol-sensitive, complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after an ovariectomy that was performed in C57BL/10 mice prior to the onset of puberty. This non-immune murine anti-A, which is complementary to the trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from the cross-reactive adaptive anti-A antibody, was strongly downregulated or did not appear in plasma of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All of the murine tissues expressed the species-specific Forssman<i> </i>reactivity, and further A-like structures were identified in the male and female reproductive and endodermal organs by reaction with innate human anti-A antibody, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by reactivity to the lectin from <i>Dolichos biflorus</i> and the agglutinin from <i>Helix pomatia </i>indicating the functional involvement of the <i>O</i>-GalNAc-determined mucin-type A-like, Tn and T (Thomsen-Friedenreich) epitopes that signify malignancy when accumulated and expressed in non-developmental tissues.
DOI: 10.6084/m9.figshare.1267558.v15
title: Traction Force Microscopy and Particle Image Velocimetry of migrating Physarum

description: This is the raw image data from simultaneous TFM and PIVy experiments performed on migrating Physarum plasmodia
DOI: 10.6084/m9.figshare.1279394.v231
title: Early ovariectomy reveals the germline encoding of the “natural” mammalian anti-A-reactive IgM reflecting developmental malignancy.

description: The germline encoding of a non-immune immunoglobulin M (IgM) molecule in mammals was experimentally documented for the first time as a result of the specifically timed ovariectomy of C57BL/10 mice. The target of this innate antibody involves a trans-species developmental antigen that signifies malignancy when expressed in any non-developmental tissue. Although ovariectomy and castration result in uncontrolled and/or enhanced humoral and cellular immunity involving increased weights of the spleen and thymus with pronounced B and T cell production, the development of mercaptoethanol-sensitive, complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after an ovariectomy that was performed in C57BL/10 mice prior to the onset of puberty. This non-immune murine anti-A, which is complementary to the trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from the cross-reactive adaptive anti-A antibody, was strongly downregulated or did not appear in plasma of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All of the murine tissues expressed the species-specific Forssman<i> </i>reactivity, and further A-like structures were identified in the male and female reproductive and endodermal organs by reaction with innate human anti-A antibody, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by reactivity to the lectin from <i>Dolichos biflorus</i> and the agglutinin from <i>Helix pomatia </i>indicating the functional involvement of the <i>O</i>-GalNAc-determined mucin-type A-like, Tn and T (Thomsen-Friedenreich) epitopes that signify malignancy when accumulated and expressed in non-developmental tissues.
DOI: 10.6084/m9.figshare.1267541.v1
title: New geological and geochronological data of the Placer de Guadalupe uplift, Mexico: a new piece of the Late Triassic–Jurassic Nazas Arc?

description: Basement exposed in the Placer de Guadalupe–Plomosas uplift in northern Mexico provides important clues for the geologic evolution of the region. The stratigraphic units form stacked thrust sheets of psammitic and calcareous formations, interlayered with magmatic rock. The eastern calcareous and quartzite formations exhibit structures associated with ductile deformation, whereas the upper stratigraphic units only contain structures formed via younger brittle deformation. Porphyry interlayered in the upper Plomosas Formation has a U-Pb zircon age of 171 ± 1 Ma. This age is consistent with its stratigraphic position, interbedded quartzarenites with a maximum depositional age of ~168 Ma. Granite flakes within the Horquilla Formation are dated at 209 ± 3 Ma, and the La Viñata quartzite exhibits a maximum age of ~193 Ma. The Upper Plomosas Formation correlates well with the arc-related Middle Jurassic Nazas Formation of northeastern Mexico, constituting the first report of a Jurassic continental margin arc outcrop in the ‘Central Mexican Gap zone’. We document Late Norian to Bajocian ages for the stratigraphic units cropping out in the Placer de Guadalupe area. The Jurassic age cluster indicates that the Nazas Arc magmatism in the region occurred during the Late Triassic and ended in the Middle Jurassic times. Permian ages previously assigned to these rocks and the occurrence of a Permo–Triassic deformation event have to be dismissed.