DOI: 10.5061/dryad.v72q6
title: Data from: Loss of adaptation following reversion suggests trade-offs in host use by a seed beetle

description: Experimental evolution has provided little support for the hypothesis that
 the narrow diets of herbivorous insects reflect trade-offs in performance
 across hosts; selection lines can sometimes adapt to an inferior novel
 host without a decline in performance on the ancestral host. An
 alternative approach for detecting trade-offs would be to measure
 adaptation decay after selection is relaxed, i.e., when populations newly
 adapted to a novel host are reverted to the ancestral one. Lines of the
 seed beetle Callosobruchus maculatus rapidly adapted to a poor host
 (lentil); survival in lentil seeds increased from 2% to &gt;90% in
 &lt;30 generations. After the lines had reached a plateau with respect
 to survival in lentil, sublines were reverted to the ancestral host, mung
 bean. Twelve generations of reversion had little effect on performance in
 lentil, but after 25-35 generations, the reverted lines exhibited lower
 survival, slower development, and smaller size. The most divergent pair of
 lines was then assayed on both lentil and mung bean. Performance on lentil
 was again much poorer in the reverted line than in the non-reverted one,
 but the lines performed equally well on mung bean. Moreover, the
 performance of the non-reverted line on mung bean remained comparable to
 that of the original mung-bean population. Our results thus present a
 paradox: loss of adaptation to lentil following reversion implies a
 trade-off, but the continued strong performance of lentil-adapted lines on
 mung bean does not. Genomic comparisons of the reverted, non-reverted, and
 ancestral lines may resolve this paradox and determine the importance of
 selection vs. drift in causing a loss of adaptation following reversion.
description: Generation 12
 assayJournal of Evolutionary
 Biology "Loss of adaptation following reversion suggests trade-offs
 in host use by a seed beetle" Messina and Durham The data for this
 paper are placed in four Excel files, one for each assay - after 12, 25,
 35, and 45 generations of reversion. For the first three files (Generation
 12-35 assays), the coding variables for the two dependent variables,
 development time and weight, are Line (1-3), Subline (NonReverted and
 Reverted), and Sex (F or M). These columns are followed by coding
 variables for the third dependent variable Survival: Line, Subline, and
 Survival (Yes or No). These columns are followed by the actual counts in
 each Y or N column. All of the dependent variables were measured solely on
 lentil seeds. For the Generation 45 assay, only the L1 and L1R lines were
 used, but beetle performance was measured on both lentil seeds and mung
 beans. The coding variables for development time and weight are Subline
 (NR or R), Host (MUNG OR LENTIL), and Sex (F or M). These columns are
 followed by coding variables for Survival: Subline (NonReverted and
 Reverted), Host (MUNG OR LENTIL), and Survival (Yes or No). These columns
 are followed by the actual counts in each Y or N
 column.Generation 25
 assayGeneration 35
 assayGeneration 45
 assay