DOI: 10.5061/dryad.x0k6djhs3
title: Data from: Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells

description: We present a study of the cellular uptake and the toxicity of core-shell
 maghemite-silica magnetic nanoparticles (MNPs) with a diameter of 9 nm.
 The uptake of the nanoparticles by mouse neuroblastoma 2a cells was
 studied quantitatively by Graphite Furnace Atomic Absorption Spectroscopy
 and SQUID magnetometry with good agreement between the methods on the
 amount of Fe oxide internalised by the cells. We were thus able to study
 the toxicity of the nanoparticles for well quantified MNP uptake in terms
 of nanoparticle density within the cell in the range of 1.7 x 105 to 1.72
 x 107 MNPs per cell. At all the exposures tested (from 0.1 ng/mL to 100
 µg/mL, for 24 hours), there was no significant variation of cell viability
 and growth rate, but studies of intracellular effects showed an increase
 in the amount of mitochondrial superoxide and increasing lysosomal
 activity due to exposure to MNPs (at 100 µg/mL). These results suggest
 that, under the most extreme conditions, we could begin to detect a
 disruption in ROS homeostasis which may lead to an impairment in
 antioxidant responses. Overall, the results demonstrate that the use of
 these MNPs, in a wide range of concentrations, is not toxic under our
 culture conditions.
description: # Data from: Toxicity and magnetometry evaluation of the uptake of
 core-shell maghemite-silica nanoparticles by neuroblastoma cells
 [https://doi.org/10.5061/dryad.x0k6djhs3](https://doi.org/10.5061/dryad.x0k6djhs3) The dataset contains 8 different files (Excel files and Origin files). The name of such files refers to the Figures in the manuscript and Supplementary Information file. Here is a brief description of the different files: **Fig1.opju** (Origin file) contains the histogram from the TEM measurements of the NPs and the fitting of the datapoints to a lognormal distribution. The file contains one worksheet (Book1) and two images from it: `Graph1` with the fitting and `Figure` with the figure used in the manuscript. **Fig2.opju** (Origin file) contains the magnetometry data for the samples under study. The worksheets’ names are self-explanatory and indicate the concentration of MNP and time of exposure. **Figure3.xlsx** (Excel file) contains the data of cell quantification after MNPs (‘Data’ sheet). The counts were performed 12 times for each sample and the counts normalised by the counts at the beginning of the experiment. **Figure5.xlsx** (Excel file) contains the counts of cells stained in four independent experiments, corresponding to the 4 different sheets of the file. Highlighted cells (B29 to B40) in ''N2A P26 21.07.2020'' sheet are unavailable. **Figure7.xlsx** (Excel file) contains the counts of the fluorescence experiments in Figure 7 of the manuscript. For the different stains, the counts of the different organelles (normalised) are shown for the control and different samples (named after the concentration of magnetic nanoparticles and exposure time), **GFAAS_data.ods** (Excel/OpenDocument file) contains the calibration curves for different wavelengths (described in the name of the sheets) and the measurements for the different samples. The samples are denoted by an ‘M ’when referring to the actual samples and ‘C’ when referring to the control samples. In the third sheet ‘Results’ the concentration of Fe in micrograms. **Negative_controls_magnetometry_(for Table_2 and Fig S1).opju** contains the magnetometry data for the negative control of the samples under study. The worksheets’ names are self-explanatory and indicate the concentration of MNP and time of exposure. **Fig S2.opju** contains the magnetic response at 300K for oleic acid-coated maghemite nanoparticles.