cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1470178.v1
title: Brook Trout Distribution, Genetics, and Population Characteristics in the Driftless Area of Minnesota

description: The Driftless Area in southeastern Minnesota is on the southwestern edge of the native range of Brook Trout <i>Salvelinus fontinalis</i>. It was assumed that native Brook Trout were extirpated from this region in the early 1900s due to degraded stream conditions and stockings of eastern-origin Brook Trout and European Brown Trout <i>Salmo trutta</i>. Our objectives were to examine Brook Trout populations in the region to determine their spatial and genetic distribution and quantify population characteristics. Information on presence or absence of Brook Trout was gathered by electrofishing 174 streams in southeastern Minnesota. Brook Trout were present in 68% of coldwater streams compared with only in 3% in the early 1970s. The increase is likely due to increasing stream discharge throughout the Driftless Area, enabling recolonization or successful establishment of stocked populations. Streams with higher base flow discharge also had higher abundance, larger size at maturity, and larger Brook Trout present. Genetic data on 74 populations were analyzed to characterize genetic variation within populations, assess genetic structure among populations, and determine possible origins. Numerous populations were not associated with known hatchery sources but were primarily composed of geographic groupings that could represent remnant lineages. Although population characteristics were similar among genetic origins, potentially remnant populations should be given conservation priority because they have proven their ability to sustain themselves in this region. Management actions that emphasize maintaining or increasing stream base flows throughout the region will likely enhance remnant Brook Trout populations in the Driftless Area.Received July 25, 2014; accepted March 17, 2015
DOI: 10.6084/m9.figshare.1470172
title: SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin

description: DNA topoisomerase II (TopoII) regulates DNA topology by its strand passaging reaction, which is required for genome maintenance by resolving tangled genomic DNA. In addition, TopoII contributes to the structural integrity of mitotic chromosomes and to the activation of cell cycle checkpoints in mitosis. Post-translational modification of TopoII is one of the key mechanisms by which its broad functions are regulated during mitosis. SUMOylation of TopoII is conserved in eukaryotes and plays a critical role in chromosome segregation. Using <i>Xenopus laevis</i> egg extract, we demonstrated previously that TopoIIα is modified by SUMO on mitotic chromosomes and that its activity is modulated via SUMOylation of its lysine at 660. However, both biochemical and genetic analyses indicated that TopoII has multiple SUMOylation sites in addition to Lys660, and the functions of the other SUMOylation sites were not clearly determined. In this study, we identified the SUMOylation sites on the C-terminal domain (CTD) of TopoIIα. CTD SUMOylation did not affect TopoIIα activity, indicating that its function is distinct from that of Lys660 SUMOylation. We found that CTD SUMOylation promotes protein binding and that Claspin, a well-established cell cycle checkpoint mediator, is one of the SUMOylation-dependent binding proteins. Claspin harbors 2 SUMO-interacting motifs (SIMs), and its robust association to mitotic chromosomes requires both the SIMs and TopoIIα-CTD SUMOylation. Claspin localizes to the mitotic centromeres depending on mitotic SUMOylation, suggesting that TopoIIα-CTD SUMOylation regulates the centromeric localization of Claspin. Our findings provide a novel mechanistic insight regarding how TopoIIα-CTD SUMOylation contributes to mitotic centromere activity.
DOI: 10.6084/m9.figshare.1470170
title: The Explorer Islamabad Issue 6

description: The Explorer islamabad 6 issue
DOI: 10.6084/m9.figshare.1470160
title: Data for: A Single Bout of Moderate Aerobic Exercise Improves Motor Skill Acquisition

description: Individual subject data for: A Single Bout of Moderate Aerobic Exercise Improves Motor Skill Acquisition
DOI: 10.6084/m9.figshare.1470170.v1
title: The Explorer Islamabad Issue 6

description: The Explorer islamabad 6 issue
DOI: 10.6084/m9.figshare.1470160.v1
title: Data for: A Single Bout of Moderate Aerobic Exercise Improves Motor Skill Acquisition

description: Individual subject data for: A Single Bout of Moderate Aerobic Exercise Improves Motor Skill Acquisition
DOI: 10.6084/m9.figshare.1470151
title: Standalone Hybrid Generation System

description: This thesis proposes a standalone hybrid generation system in Thar area of Pakistan combining solar and wind energy with provision of a storage bank and controlled using a bidirectional boost DC-DC converter (BDC) to maintain constant dc-link voltage. A threephase complex vector control scheme voltage source inverter (VSI) is used to control the load side voltage in terms of the frequency and voltage amplitude. The simulation results obtained from Matlab/Simulink show that the overall hybrid framework is capable of working under the variable weather and load conditions.
DOI: 10.6084/m9.figshare.1470151.v1
title: Standalone Hybrid Generation System

description: This thesis proposes a standalone hybrid generation system in Thar area of Pakistan combining solar and wind energy with provision of a storage bank and controlled using a bidirectional boost DC-DC converter (BDC) to maintain constant dc-link voltage. A threephase complex vector control scheme voltage source inverter (VSI) is used to control the load side voltage in terms of the frequency and voltage amplitude. The simulation results obtained from Matlab/Simulink show that the overall hybrid framework is capable of working under the variable weather and load conditions.
DOI: 10.6084/m9.figshare.1470172.v1
title: SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin

description: DNA topoisomerase II (TopoII) regulates DNA topology by its strand passaging reaction, which is required for genome maintenance by resolving tangled genomic DNA. In addition, TopoII contributes to the structural integrity of mitotic chromosomes and to the activation of cell cycle checkpoints in mitosis. Post-translational modification of TopoII is one of the key mechanisms by which its broad functions are regulated during mitosis. SUMOylation of TopoII is conserved in eukaryotes and plays a critical role in chromosome segregation. Using <i>Xenopus laevis</i> egg extract, we demonstrated previously that TopoIIα is modified by SUMO on mitotic chromosomes and that its activity is modulated via SUMOylation of its lysine at 660. However, both biochemical and genetic analyses indicated that TopoII has multiple SUMOylation sites in addition to Lys660, and the functions of the other SUMOylation sites were not clearly determined. In this study, we identified the SUMOylation sites on the C-terminal domain (CTD) of TopoIIα. CTD SUMOylation did not affect TopoIIα activity, indicating that its function is distinct from that of Lys660 SUMOylation. We found that CTD SUMOylation promotes protein binding and that Claspin, a well-established cell cycle checkpoint mediator, is one of the SUMOylation-dependent binding proteins. Claspin harbors two SUMO-interacting motifs (SIMs), and its robust association to mitotic chromosomes requires both the SIMs and TopoIIα-CTD SUMOylation. Claspin localizes to the mitotic centromeres depending on mitotic SUMOylation, suggesting that TopoIIα-CTD SUMOylation regulates the centromeric localization of Claspin. Our findings provide a novel mechanistic insight regarding how TopoIIα-CTD SUMOylation contributes to mitotic centromere activity.
DOI: 10.6084/m9.figshare.1470149
title: Example slice of diffusion tensor image

description: Example 2D slice of DTI in human brain
DOI: 10.6084/m9.figshare.1470172.v2
title: SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin

description: DNA topoisomerase II (TopoII) regulates DNA topology by its strand passaging reaction, which is required for genome maintenance by resolving tangled genomic DNA. In addition, TopoII contributes to the structural integrity of mitotic chromosomes and to the activation of cell cycle checkpoints in mitosis. Post-translational modification of TopoII is one of the key mechanisms by which its broad functions are regulated during mitosis. SUMOylation of TopoII is conserved in eukaryotes and plays a critical role in chromosome segregation. Using <i>Xenopus laevis</i> egg extract, we demonstrated previously that TopoIIα is modified by SUMO on mitotic chromosomes and that its activity is modulated via SUMOylation of its lysine at 660. However, both biochemical and genetic analyses indicated that TopoII has multiple SUMOylation sites in addition to Lys660, and the functions of the other SUMOylation sites were not clearly determined. In this study, we identified the SUMOylation sites on the C-terminal domain (CTD) of TopoIIα. CTD SUMOylation did not affect TopoIIα activity, indicating that its function is distinct from that of Lys660 SUMOylation. We found that CTD SUMOylation promotes protein binding and that Claspin, a well-established cell cycle checkpoint mediator, is one of the SUMOylation-dependent binding proteins. Claspin harbors 2 SUMO-interacting motifs (SIMs), and its robust association to mitotic chromosomes requires both the SIMs and TopoIIα-CTD SUMOylation. Claspin localizes to the mitotic centromeres depending on mitotic SUMOylation, suggesting that TopoIIα-CTD SUMOylation regulates the centromeric localization of Claspin. Our findings provide a novel mechanistic insight regarding how TopoIIα-CTD SUMOylation contributes to mitotic centromere activity.
DOI: 10.6084/m9.figshare.1470149.v1
title: Example slice of diffusion tensor image

description: Example 2D slice of DTI in human brain
DOI: 10.6084/m9.figshare.1470172.v3
title: SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin

description: DNA topoisomerase II (TopoII) regulates DNA topology by its strand passaging reaction, which is required for genome maintenance by resolving tangled genomic DNA. In addition, TopoII contributes to the structural integrity of mitotic chromosomes and to the activation of cell cycle checkpoints in mitosis. Post-translational modification of TopoII is one of the key mechanisms by which its broad functions are regulated during mitosis. SUMOylation of TopoII is conserved in eukaryotes and plays a critical role in chromosome segregation. Using <i>Xenopus laevis</i> egg extract, we demonstrated previously that TopoIIα is modified by SUMO on mitotic chromosomes and that its activity is modulated via SUMOylation of its lysine at 660. However, both biochemical and genetic analyses indicated that TopoII has multiple SUMOylation sites in addition to Lys660, and the functions of the other SUMOylation sites were not clearly determined. In this study, we identified the SUMOylation sites on the C-terminal domain (CTD) of TopoIIα. CTD SUMOylation did not affect TopoIIα activity, indicating that its function is distinct from that of Lys660 SUMOylation. We found that CTD SUMOylation promotes protein binding and that Claspin, a well-established cell cycle checkpoint mediator, is one of the SUMOylation-dependent binding proteins. Claspin harbors 2 SUMO-interacting motifs (SIMs), and its robust association to mitotic chromosomes requires both the SIMs and TopoIIα-CTD SUMOylation. Claspin localizes to the mitotic centromeres depending on mitotic SUMOylation, suggesting that TopoIIα-CTD SUMOylation regulates the centromeric localization of Claspin. Our findings provide a novel mechanistic insight regarding how TopoIIα-CTD SUMOylation contributes to mitotic centromere activity.
DOI: 10.6084/m9.figshare.1470149.v2
title: Example slice of diffusion tensor image

description: Example 2D slice of DTI in human brain
DOI: 10.6084/m9.figshare.1470035
title: Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Figures S1-S7

description: <strong>Estrogen receptor alpha (<em>ESR1</em>) gene amplification is frequent in breast cancer. Subsequent supplementary material.</strong> Frederik Holst & Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (<em>ESR1</em>) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). <strong>Subsequent Supplementary Figures S1-S7</strong> Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented <em>ESR1</em> copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of <em>ESR1</em> FISH analysis signal appearance of tumors with <em>ESR1</em> amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 <strong>Subsequent Supplementary Video Clips S1+S2</strong> The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 <strong>Subsequent Supplementary Optical Dataset S1</strong> The optical dataset consists of 94 photographs that show the appearance of <i>ESR1</i> FISH signals of 20 out of 22 tumors in regard to the GeneChip analysis that is illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 <strong>Methods References</strong> Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. & van Diest, P.J. <em>ESR1</em> Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of <em>ESR1</em> Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).
DOI: 10.6084/m9.figshare.1470172.v4
title: SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin

description: DNA topoisomerase II (TopoII) regulates DNA topology by its strand passaging reaction, which is required for genome maintenance by resolving tangled genomic DNA. In addition, TopoII contributes to the structural integrity of mitotic chromosomes and to the activation of cell cycle checkpoints in mitosis. Post-translational modification of TopoII is one of the key mechanisms by which its broad functions are regulated during mitosis. SUMOylation of TopoII is conserved in eukaryotes and plays a critical role in chromosome segregation. Using <i>Xenopus laevis</i> egg extract, we demonstrated previously that TopoIIα is modified by SUMO on mitotic chromosomes and that its activity is modulated via SUMOylation of its lysine at 660. However, both biochemical and genetic analyses indicated that TopoII has multiple SUMOylation sites in addition to Lys660, and the functions of the other SUMOylation sites were not clearly determined. In this study, we identified the SUMOylation sites on the C-terminal domain (CTD) of TopoIIα. CTD SUMOylation did not affect TopoIIα activity, indicating that its function is distinct from that of Lys660 SUMOylation. We found that CTD SUMOylation promotes protein binding and that Claspin, a well-established cell cycle checkpoint mediator, is one of the SUMOylation-dependent binding proteins. Claspin harbors 2 SUMO-interacting motifs (SIMs), and its robust association to mitotic chromosomes requires both the SIMs and TopoIIα-CTD SUMOylation. Claspin localizes to the mitotic centromeres depending on mitotic SUMOylation, suggesting that TopoIIα-CTD SUMOylation regulates the centromeric localization of Claspin. Our findings provide a novel mechanistic insight regarding how TopoIIα-CTD SUMOylation contributes to mitotic centromere activity.
DOI: 10.6084/m9.figshare.1470130
title: Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent supplementary optical dataset S1

description: <strong>Estrogen receptor alpha (<em>ESR1</em>) gene amplification is frequent in breast cancer. Subsequent supplementary material.</strong> Frederik Holst & Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (<em>ESR1</em>) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). <strong>Subsequent Supplementary Figures S1-S7</strong> Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented <em>ESR1</em> copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of <em>ESR1</em> FISH analysis signal appearance of tumors with <em>ESR1</em> amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 <strong>Subsequent Supplementary Video Clips S1+S2</strong> The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 <strong>Subsequent Supplementary Optical Dataset S1</strong> The optical dataset consists of 94 photographs that show the appearance of <i>ESR1</i> FISH signals of 20 out of 22 tumors in regard to the GeneChip analysis that is illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 <strong>Methods References</strong> Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. & van Diest, P.J. <em>ESR1</em> Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of <em>ESR1</em> Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).
DOI: 10.6084/m9.figshare.1469935
title: Can static habitat protection encompass critical areas for highly mobile marine top predators? Insights from coastal East Africa

description: This file contains sightings and group size data collected by GVI and KWS on the Indo-Pacific bottlenose dolphin population from 2006 to 2009 in southern Kenya . The data is organized by grids of 1km2. The latitude and longitude are the centroid of each grid. A total of 10 environmental variables (5 dynamic and 5 static) are included for each grid during each season. Only grids with a minimum effort on each season are included on this file and for this reason different grids were sampled over the study period. Sightings and group size data are provided on absolute number and binomial (presence/absence).
DOI: 10.6084/m9.figshare.1470034
title: Questionnaire on RadioMarche Usage Nov/Oct 2012

description: RadioMarche feedback report.
DOI: 10.6084/m9.figshare.1469935.v1
title: Can static habitat protection encompass critical areas for highly mobile marine top predators? Insights from coastal East Africa

description: This file contains sightings and group size data collected by GVI and KWS on the Indo-Pacific bottlenose dolphin population from 2006 to 2009 in southern Kenya . The data is organized by grids of 1km2. The latitude and longitude are the centroid of each grid. A total of 10 environmental variables (5 dynamic and 5 static) are included for each grid during each season. Only grids with a minimum effort on each season are included on this file and for this reason different grids were sampled over the study period. Sightings and group size data are provided on absolute number and binomial (presence/absence).
DOI: 10.6084/m9.figshare.1470035.v2
title: Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Figures S1-S7

description: <strong>Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Material.</strong> Frederik Holst & Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (ESR1) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). <strong>Subsequent Supplementary Figures S1-S7</strong> Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented <em>ESR1</em> copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of <em>ESR1</em> FISH analysis signal appearance of tumors with <em>ESR1</em> amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 <strong>Subsequent Supplementary Video Clips S1+S2</strong> The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 <strong>Subsequent Supplementary Optical Dataset S1</strong> The optical dataset consists of 94 photographs documenting appearance of <em>ESR1</em> FISH analysis signals of 20 out of 22 tumors regarding SNP GeneChip analysis illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 <strong>Methods References</strong> Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. & van Diest, P.J. ESR1 Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of ESR1 Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).
DOI: 10.6084/m9.figshare.1469659
title: Studies of wild bird populations have shown that exploratory behaviour is associated with polymorphisms in DRD4. Additionally high levels of anxiety, a characteristic of bold behaviour, and exploratory behaviour have been associated with polymorphisms in SERT.

description: Studies of wild bird populations have shown that exploratory behaviour is associated with polymorphisms in DRD4. Additionally high levels of anxiety, a characteristic of bold behaviour, and exploratory behaviour have been associated with polymorphisms in SERT. Here we tested for polymorphisms in DRD4 and SERT in the Seychelles warbler (Acrocephalus sechellensis) population on Cousin Island, Seychelles, and then investigated correlations between personality and polymorphisms in these genes.