cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.3364204.v1
title: Marine yeast biodiversity on seaweeds in New Zealand waters

description: Little is known about the biodiversity of marine yeast from New Zealand. Marine yeasts were isolated from various seaweed surfaces sampled at three locations in the Wellington Region. DNA sequencing of the internal transcribed spacer (ITS) regions was conducted, and the resultant sequence data were used in isolate identification. Yeasts isolated during this investigation were not unique; 71 isolates that are frequently detected in marine and terrestrial environments worldwide were identified from a range of seaweed genera. Furthermore, high ITS sequence similarity was observed between yeasts isolated during this investigation and those from geographically distant locations. These findings may indicate that marine yeasts are ubiquitous at a global level, although evidence is insufficient as to whether yeasts demonstrate biogeographic distribution patterns. Yeasts isolated during this investigation may have ecological implications in New Zealand's marine environment. Yeast isolates identified from seaweed genera have been demonstrated to have beneficial properties and applications and their potential should be explored.
DOI: 10.6084/m9.figshare.3364033
title: 2015 My Journey Into Glass

description: This article published in the IEEE Consumer Electronics Magazine, Volume 5, No. 1 January 2016 is a series of excerpts from 10 differing Google Glass Explorers who were interviewed by Alexander Hayes through 2014. More information and the entire web accessible interview playlist is available at https://goo.gl/PTCnhw
DOI: 10.6084/m9.figshare.3364033.v1
title: 2015 My Journey Into Glass

description: This article published in the IEEE Consumer Electronics Magazine, Volume 5, No. 1 January 2016 is a series of excerpts from 10 differing Google Glass Explorers who were interviewed by Alexander Hayes through 2014. More information and the entire web accessible interview playlist is available at https://goo.gl/PTCnhw
DOI: 10.6084/m9.figshare.3363979
title: Synthesis of pyrrol-pyridazyl-triazolyl-pyridines via Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction

description: Conjugated alternative donor–acceptor type pyrrol-pyridazyl-triazolyl-pyridine <i>N</i>-heterocyclic systems were synthesized via Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction of pyrrol-pyridazylacetylene with azidopyridines.
DOI: 10.6084/m9.figshare.3363979.v1
title: Synthesis of Pyrrol-Pyridazyl-Triazolyl-Pyridines via Cu(I) Catalyzed Azide-Alkyne 1,3-Dipolar Cycloaddition Reaction

description: Conjugated alternative donor-acceptor type pyrrol-pyridazyl-triazolyl-pyridine<i>N</i>-heterocyclic systems were synthesized via Cu(I) catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction of pyrrol-pyridazylacetylene with azidopyridines.
DOI: 10.6084/m9.figshare.3363979.v2
title: Synthesis of pyrrol-pyridazyl-triazolyl-pyridines via Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction

description: Conjugated alternative donor–acceptor type pyrrol-pyridazyl-triazolyl-pyridine <i>N</i>-heterocyclic systems were synthesized via Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction of pyrrol-pyridazylacetylene with azidopyridines.
DOI: 10.6084/m9.figshare.3363967
title: Synthesis of three putative kairomones of the beech leaf-mining weevil <i>Orchestes fagi</i> (L.)

description: The beech leaf-mining weevil, <i>Orchestes fagi</i> (L.), also known as the beech flea weevil, is a common and widespread pest of beech, <i>Fagus sylvatica</i> L., in its native Europe. It now appears to be well established in Nova Scotia, Canada. We report a novel synthesis of 9-geranyl-p-cymene and syntheses of 9-geranyl-α-terpinene and 1,1-dimethyl-3-methylene-2-vinylcyclohexane, making partial use of known methods. All three of these compounds are found in beech leaf volatiles and/or wood and are putative kairomones of the beech leaf-mining weevil.
DOI: 10.6084/m9.figshare.3363967.v1
title: Synthesis of Three Putative Kairomones of the Beech Leaf-Mining Weevil <i>Orchestes fagi</i> (L.)

description: The beech leaf-mining weevil, Orchestes fagi (L.), also known as the beech flea weevil, is a common and widespread pest of beech, Fagus sylvatica L., in its native Europe. It now appears to be well established in Nova Scotia, Canada. We report a novel synthesis of 9-geranyl-p-cymene, and syntheses of 9-geranyl-α -terpinene and 1,1-dimethyl-3-methylene-2-vinylcyclohexane making partial use of known methods. All three of these compounds are found in beech leaf volatiles and / or wood, and are putative kairomones of the beech leaf-mining weevil.
DOI: 10.6084/m9.figshare.3363967.v2
title: Synthesis of three putative kairomones of the beech leaf-mining weevil <i>Orchestes fagi</i> (L.)

description: The beech leaf-mining weevil, <i>Orchestes fagi</i> (L.), also known as the beech flea weevil, is a common and widespread pest of beech, <i>Fagus sylvatica</i> L., in its native Europe. It now appears to be well established in Nova Scotia, Canada. We report a novel synthesis of 9-geranyl-p-cymene and syntheses of 9-geranyl-α-terpinene and 1,1-dimethyl-3-methylene-2-vinylcyclohexane, making partial use of known methods. All three of these compounds are found in beech leaf volatiles and/or wood and are putative kairomones of the beech leaf-mining weevil.
DOI: 10.6084/m9.figshare.3363961
title: Lavalatte_distribution_occurrence_examples.rar

description: Examples of occurrence of the Lavalette distributionSAU population for Nigeria.TAU population in Spain.Amino acid to amino acid mutation rates for missense variants.Plant organelle codon usage.Non rodent mammalian nucleus codon usage.<br>
DOI: 10.6084/m9.figshare.3363961.v2
title: Lavalatte_distribution_occurrence_examples.rar

description: Examples of occurrence of the Lavalette distributionSAU population for Nigeria.TAU population in Spain.Amino acid to amino acid mutation rates for missense variants.Plant organelle codon usage.Non rodent mammalian nucleus codon usage.<br>
DOI: 10.6084/m9.figshare.3363961.v1
title: Data.rar

description: SAU population for Nigeria.TAU population in Spain.Amino acid to amino acid mutation rates for missense variants.Plant organelle codon usage.Non rodent mammalian nucleus codon usage.<br>
DOI: 10.6084/m9.figshare.3363958.v1
title: JUB1 suppresses <i>Pseudomonas syringae</i>-induced defense responses through accumulation of DELLA proteins

description: Phytohormones act in concert to coordinate plant growth and the response to environmental cues. Gibberellins (GAs) are growth-promoting hormones that recently emerged as modulators of plant immune signaling. By regulating the stability of DELLA proteins, GAs intersect with the signaling pathways of the classical primary defense hormones, salicylic acid (SA) and jasmonic acid (JA), thereby altering the final outcome of the immune response. DELLA proteins confer resistance to necrotrophic pathogens by potentiating JA signaling and raise the susceptibility to biotrophic pathogens by attenuating the SA pathway. Here, we show that JUB1, a core element of the GA - brassinosteroid (BR) - DELLA regulatory module, functions as a negative regulator of defense responses against <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 (<i>Pst</i> DC3000) and mediates the crosstalk between growth and immunity.
DOI: 10.6084/m9.figshare.3363952
title: Synthesis of GR 125487, a selective 5-HT<sub>4</sub> receptor antagonist

description: The 5-HT<sub>4</sub> receptor (5-HT<sub>4</sub>R) agonists are important in treating gastrointestinal motility disorders. Their role is currently being evaluated for the treatment of cognitive and mood disorders. A selective 5-HT<sub>4</sub>R antagonist GR 125487 is used in many biological assays to cross confirm the 5-HT<sub>4</sub>R agonist’s activity. A practical synthesis of GR 125487 is developed so as to have it in desired quantities. The synthesis consists of seven steps starting from commercially available methyl 5-fluoroindole 3-carboxylate. The GR 125487 thus synthesized was used in blocking the activity of 5-HT<sub>4</sub>R agonist compound in animal models of cognition.
DOI: 10.6084/m9.figshare.3363952.v2
title: Synthesis of GR 125487, a selective 5-HT<sub>4</sub> receptor antagonist

description: The 5-HT<sub>4</sub> receptor (5-HT<sub>4</sub>R) agonists are important in treating gastrointestinal motility disorders. Their role is currently being evaluated for the treatment of cognitive and mood disorders. A selective 5-HT<sub>4</sub>R antagonist GR 125487 is used in many biological assays to cross confirm the 5-HT<sub>4</sub>R agonist’s activity. A practical synthesis of GR 125487 is developed so as to have it in desired quantities. The synthesis consists of seven steps starting from commercially available methyl 5-fluoroindole 3-carboxylate. The GR 125487 thus synthesized was used in blocking the activity of 5-HT<sub>4</sub>R agonist compound in animal models of cognition.
DOI: 10.6084/m9.figshare.3363952.v1
title: Synthesis of GR 125487 a Selective 5-HT<sub>4</sub> Receptor Antagonist

description: The 5-HT<sub>4</sub> receptor (5-HT<sub>4</sub>R) agonists are important in treating gastrointestinal motility disorders. Their role is currently being evaluated for the treatment of cognitive and mood disorders. A selective 5-HT<sub>4</sub>R antagonist GR 125487 is used in many biological assays to cross confirm the 5-HT<sub>4</sub>R agonist's activity. A practical synthesis of GR 125487 is developed so as to have it in desired quantities. The synthesis consists of seven steps starting from commercially available methyl 5-fluoroindole 3-carboxylate. The GR 125487 thus synthesized was used in blocking the activity of 5-HT<sub>4</sub>R agonist compound in animal models of cognition.
DOI: 10.6084/m9.figshare.3363949
title: Synthesis, photophysical, electrochemical and single-crystal x-ray diffraction study of (<i>Z</i>)-2-phenyl-3-(5-(4-(thiophen-2-yl)benzo[c][1,2,5]thiadiazol-7-yl)thiophen-2-yl)acrylonitrile

description: The optical characteristics, redox properties, thermogravimetric stability and single-crystal X-ray diffraction study of (<i>Z</i>)-2-phenyl-3-(5-(4-(thiophen-2-yl)benzo[c][1,2,5]thiadiazol-7-yl)thiophen-2-yl)acrylonitrile are examined using ultraviolet–visible spectrophotometry, cyclic voltammetry, thermal gravimetric analysis–diffraction scanning calorimetry analysis, single-crystal X-ray diffraction and density functional theory calculations. Evidently, the crystal structure of compound <b>6</b> is sustained by a number of weak nonconventional intermolecular forces of attraction such as C-H … N, C-H … π donor–acceptor interactions.
DOI: 10.6084/m9.figshare.3363949.v2
title: Synthesis, photophysical, electrochemical and single-crystal x-ray diffraction study of (<i>Z</i>)-2-phenyl-3-(5-(4-(thiophen-2-yl)benzo[c][1,2,5]thiadiazol-7-yl)thiophen-2-yl)acrylonitrile

description: The optical characteristics, redox properties, thermogravimetric stability and single-crystal X-ray diffraction study of (<i>Z</i>)-2-phenyl-3-(5-(4-(thiophen-2-yl)benzo[c][1,2,5]thiadiazol-7-yl)thiophen-2-yl)acrylonitrile are examined using ultraviolet–visible spectrophotometry, cyclic voltammetry, thermal gravimetric analysis–diffraction scanning calorimetry analysis, single-crystal X-ray diffraction and density functional theory calculations. Evidently, the crystal structure of compound <b>6</b> is sustained by a number of weak nonconventional intermolecular forces of attraction such as C-H … N, C-H … π donor–acceptor interactions.
DOI: 10.6084/m9.figshare.3363949.v1
title: Synthesis, Photophysical, Electrochemical and Single Crystal X-Ray Diffraction Study of (<i>Z</i>)-2-phenyl-3-(5-(4-(thiophen-2-yl)benzo[c][1,2,5]thiadiazol-7-yl)thiophen-2-yl)acrylonitrile

description: The optical characteristics, redox properties, thermogravimetric stability and single-crystal X-ray diffraction study of (<i>Z</i>)-2-phenyl-3-(5-(4-(thiophen-2-yl)benzo[c][1,2,5]thiadiazol-7-yl)thiophen-2-yl)acrylonitrile are examined using UV-Vis spectrophotometry, cyclic voltammetry, TGA-DSC analysis, single crystal X-ray diffraction and density functional theory calculations. Evidently, the crystal structure of compound <b>6</b> is sustained by a number of weak non-conventional intermolecular forces of attraction such as C-H … N, C-H…π donor-acceptor interactions.
DOI: 10.6084/m9.figshare.3363940
title: Defective recognition of LC3B by mutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism in ALS-FTLD

description: Growing evidence implicates impairment of autophagy as a candidate pathogenic mechanism in the spectrum of neurodegenerative disorders which includes amyotrophic lateral sclerosis and frontotemporal lobar degeneration (ALS-FTLD). <i>SQSTM1</i>, which encodes the autophagy receptor SQSTM1/p62, is genetically associated with ALS-FTLD, although to date autophagy-relevant functional defects in disease-associated variants have not been described. A key protein-protein interaction in autophagy is the recognition of a lipid-anchored form of LC3 (LC3-II) within the phagophore membrane by SQSTM1, mediated through its LC3-interacting region (LIR), and notably some ALS-FTLD mutations map to this region. Here we show that although representing a conservative substitution and predicted to be benign, the ALS-associated L341V mutation of SQSTM1 is defective in recognition of LC3B. We place our observations on a firm quantitative footing by showing the L341V-mutant LIR is associated with a ∼3-fold reduction in LC3B binding affinity and using protein NMR we rationalize the structural basis for the effect. This functional deficit is realized in motor neuron-like cells, with the L341V mutant EGFP-mCherry-SQSTM1 less readily incorporated into acidic autophagic vesicles than the wild type. Our data supports a model in which the L341V mutation limits the critical step of SQSTM1 recruitment to the phagophore. The oligomeric nature of SQSTM1, which presents multiple LIRs to template growth of the phagophore, potentially gives rise to avidity effects which amplify the relatively modest impact of any single mutation on LC3B binding. Over the lifetime of a neuron, impaired autophagy could expose a vulnerability, which ultimately tips the balance from cell survival toward cell death.
DOI: 10.6084/m9.figshare.3363940.v1
title: Defective recognition of LC3B by mutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism in ALS-FTLD

description: Growing evidence implicates impairment of autophagy as a candidate pathogenic mechanism in the spectrum of neurodegenerative disorders which includes amyotrophic lateral sclerosis and frontotemporal lobar degeneration (ALS-FTLD). <i>SQSTM1</i>, which encodes the autophagy receptor SQSTM1/p62, is genetically associated with ALS-FTLD, although to date autophagy-relevant functional defects in disease-associated variants have not been described. A key protein-protein interaction in autophagy is the recognition of a lipid-anchored form of LC3 (LC3-II) within the phagophore membrane by SQSTM1, mediated through its LC3-interacting region (LIR), and notably some ALS-FTLD mutations map to this region. Here we show that although representing a conservative substitution and predicted to be benign, the ALS-associated L341V mutation of SQSTM1 is defective in recognition of LC3B. We place our observations on a firm quantitative footing by showing the L341V-mutant LIR is associated with a ∼3-fold reduction in LC3B binding affinity and using protein NMR we rationalize the structural basis for the effect. This functional deficit is realized in motor neuron-like cells, with the L341V mutant EGFP-mCherry-SQSTM1 less readily incorporated into acidic autophagic vesicles than the wild type. Our data supports a model in which the L341V mutation limits the critical step of SQSTM1 recruitment to the phagophore. The oligomeric nature of SQSTM1, which presents multiple LIRs to template growth of the phagophore, potentially gives rise to avidity effects which amplify the relatively modest impact of any single mutation on LC3B binding. Over the lifetime of a neuron, impaired autophagy could expose a vulnerability, which ultimately tips the balance from cell survival toward cell death.
DOI: 10.6084/m9.figshare.3363925
title: Prediction of preeclampsia with angiogenic biomarkers. Results from the prospective Odense Child Cohort

description: <i>Objective</i>: We aimed to investigate how maternal serum soluble Fms-like kinase 1 (sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio prospectively associate to preeclampsia (PE) and clinical subtypes. <i>Methods</i>: In an unselected cohort of 1909 pregnant women, sFlt-1 and PlGF were measured with KRYPTOR assays in gestational weeks (GW) 8–14 and 20–34. Associations to PE were assessed by receiver operating characteristics and logistic regression. <i>Results</i>: Concentrations of sFlt-1, PlGF, and sFlt-1/PlGF in GW20-34 were predictive of PE development, but not in GW8-14. PlGF outperformed sFlt-1/PlGF ratio with an area under curve (AUC) of 0.755 vs. 0.704, <i>p</i> = 0.002. The highest AUC values for PlGF and sFlt-1/PlGF ratio were seen for severe early-onset PE (0.901 and 0.883). Negative predictive values were high for all PE types, but positive predictive values were low. <i>Conclusion</i>: PlGF and sFlt-1/PlGF had good predictive value for PE at GW20-34 in a population-based unselected cohort, however with low positive predictive value.
DOI: 10.6084/m9.figshare.3363925.v1
title: Prediction of preeclampsia with angiogenic biomarkers. Results from the prospective Odense Child Cohort

description: <i>Objective</i>: We aimed to investigate how maternal serum soluble Fms-like kinase 1 (sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio prospectively associate to preeclampsia (PE) and clinical subtypes. <i>Methods</i>: In an unselected cohort of 1909 pregnant women, sFlt-1 and PlGF were measured with KRYPTOR assays in gestational weeks (GW) 8–14 and 20–34. Associations to PE were assessed by receiver operating characteristics and logistic regression. <i>Results</i>: Concentrations of sFlt-1, PlGF, and sFlt-1/PlGF in GW20-34 were predictive of PE development, but not in GW8-14. PlGF outperformed sFlt-1/PlGF ratio with an area under curve (AUC) of 0.755 vs. 0.704, <i>p</i> = 0.002. The highest AUC values for PlGF and sFlt-1/PlGF ratio were seen for severe early-onset PE (0.901 and 0.883). Negative predictive values were high for all PE types, but positive predictive values were low. <i>Conclusion</i>: PlGF and sFlt-1/PlGF had good predictive value for PE at GW20-34 in a population-based unselected cohort, however with low positive predictive value.
DOI: 10.6084/m9.figshare.3363913
title: Wip1 phosphatase modulates both long-term potentiation and long-term depression through the dephosphorylation of CaMKII

description: Synaptic plasticity is an important mechanism that underlies learning and cognition. Protein phosphorylation by kinases and dephosphorylation by phosphatases play critical roles in the activity-dependent alteration of synaptic plasticity. In this study, we report that Wip1, a protein phosphatase, is essential for long-term potentiation (LTP) and long-term depression (LTD) processes. Wip1-deletion suppresses LTP and enhances LTD in the hippocampus CA1 area. Wip1 deficiency-induced aberrant elevation of CaMKII T286/287 and T305 phosphorylation underlies these dysfunctions. Moreover, we showed that Wip1 modulates CaMKII dephosphorylation. Wip1<sup>−/−</sup> mice exhibit abnormal GluR1 membrane expression, which could be reversed by the application of a CaMKII inhibitor, indicating that Wip1/CaMKII signaling is crucial for synaptic plasticity. Together, our results demonstrate that Wip1 phosphatase plays a vital role in regulating hippocampal synaptic plasticity by modulating the phosphorylation of CaMKII.
DOI: 10.6084/m9.figshare.3363913.v1
title: Wip1 phosphatase modulates both long-term potentiation and long-term depression through the dephosphorylation of CaMKII

description: Synaptic plasticity is an important mechanism that underlies learning and cognition. Protein phosphorylation by kinases and dephosphorylation by phosphatases play critical roles in the activity-dependent alteration of synaptic plasticity. In this study, we report that Wip1, a protein phosphatase, is essential for long-term potentiation (LTP) and long-term depression (LTD) processes. Wip1-deletion suppresses LTP and enhances LTD in the hippocampus CA1 area. Wip1 deficiency-induced aberrant elevation of CaMKII T286/287 and T305 phosphorylation underlies these dysfunctions. Moreover, we showed that Wip1 modulates CaMKII dephosphorylation. Wip1<sup>−/−</sup> mice exhibit abnormal GluR1 membrane expression, which could be reversed by the application of a CaMKII inhibitor, indicating that Wip1/CaMKII signaling is crucial for synaptic plasticity. Together, our results demonstrate that Wip1 phosphatase plays a vital role in regulating hippocampal synaptic plasticity by modulating the phosphorylation of CaMKII.
DOI: 10.6084/m9.figshare.3363895
title: Characterization of bacterial communities associated with <i>Brassica napus</i> L. growing on a Zn-contaminated soil and their effects on root growth

description: The interaction between plant growth-promoting bacteria (PGPB) and plants can enhance biomass production and metal tolerance of the host plants. This work aimed at isolating and characterizing the cultivable bacterial community associated with <i>Brassica napus</i> growing on a Zn-contaminated site, for selecting cultivable PGPB that might enhance biomass production and metal tolerance of energy crops. The effects of some of these bacterial strains on root growth of <i>B. napus</i> exposed to increasing Zn and Cd concentrations were assessed. A total of 426 morphologically different bacterial strains were isolated from the soil, the rhizosphere, and the roots and stems of <i>B. napus</i>. The diversity of the isolated bacterial populations was similar in rhizosphere and roots, but lower in soil and stem compartments. <i>Burkoholderia, Alcaligenes, Agrococcus, Polaromonas, Stenotrophomonas, Serratia, Microbacterium</i>, and <i>Caulobacter</i> were found as root endophytes exclusively. The inoculation of seeds with <i>Pseudomonas</i> sp. strains 228 and 256, and <i>Serratia</i> sp. strain 246 facilitated the root development of <i>B. napus</i> at 1,000 µM Zn. <i>Arthrobacter</i> sp. strain 222, <i>Serratia</i> sp. strain 246, and <i>Pseudomonas</i> sp. 228 and 262 increased the root length at 300 µM Cd.
DOI: 10.6084/m9.figshare.3363895.v2
title: Characterization of bacterial communities associated with <i>Brassica napus</i> L. growing on a Zn-contaminated soil and their effects on root growth

description: The interaction between plant growth-promoting bacteria (PGPB) and plants can enhance biomass production and metal tolerance of the host plants. This work aimed at isolating and characterizing the cultivable bacterial community associated with <i>Brassica napus</i> growing on a Zn-contaminated site, for selecting cultivable PGPB that might enhance biomass production and metal tolerance of energy crops. The effects of some of these bacterial strains on root growth of <i>B. napus</i> exposed to increasing Zn and Cd concentrations were assessed. A total of 426 morphologically different bacterial strains were isolated from the soil, the rhizosphere, and the roots and stems of <i>B. napus</i>. The diversity of the isolated bacterial populations was similar in rhizosphere and roots, but lower in soil and stem compartments. <i>Burkoholderia, Alcaligenes, Agrococcus, Polaromonas, Stenotrophomonas, Serratia, Microbacterium</i>, and <i>Caulobacter</i> were found as root endophytes exclusively. The inoculation of seeds with <i>Pseudomonas</i> sp. strains 228 and 256, and <i>Serratia</i> sp. strain 246 facilitated the root development of <i>B. napus</i> at 1,000 µM Zn. <i>Arthrobacter</i> sp. strain 222, <i>Serratia</i> sp. strain 246, and <i>Pseudomonas</i> sp. 228 and 262 increased the root length at 300 µM Cd.