DOI: 10.5061/dryad.nzs7h452g
title: nrDNA internal and external transcribed spacer sequences for investigating the systematics of Dieteria

description: The objectives of this research were to assess variation in nrDNA internal
 and external transcribed spacer (ITS and ETS) sequences of the
 morphologically diverse genus Dieteria and to determine how well the
 results of phylogenetic analysis corresponded with morphological
 variation, taxonomy, and geographic distribution. The first of two
 analyses included 39 samples from throughout the range of the genus
 representing 13 of the 16 currently accepted varieties, with ITS and ETS
 sequences combined to obtain the greatest resolution possible. This
 analysis produced phylogenies containing three major clades. One of the
 three consisted of samples from Washington, Oregon, and northern
 California. The second one included samples from southern California and
 western Arizona, plus additional ones from northern California, northern
 Nevada, and southeastern Idaho. The third clade was the largest and
 consisted of samples from the remaining part of the distribution of
 Dieteria. Comparison of the results with current taxonomy and
 morphological characteristics showed little correspondence between the
 molecular phylogeny and either taxonomy or morphology, suggesting multiple
 occurrences of convergent evolution. There were some correlations with
 geographic distributions. Except for the second and third clades’
 distributions overlapping in some areas, the distributions of the three
 were mostly distinct from one another; smaller clades within them were
 also correlated with distributions. The second analysis included cloned
 sequences of five additional samples that were shown by initial sequencing
 efforts to contain many polymorphic nucleotide positions. In the results,
 the ITS and/or ETS for all five samples were placed in multiple positions
 some distance apart, indicating that these samples obtained their ITS
 and/or ETS sequences from distantly related ancestors through
 hybridization between members of two of the three major clades.
description: # nrDNA internal and external transcribed spacer sequences for
 investigating the systematics of Dieteria Dataset DOI:
 [10.5061/dryad.nzs7h452g](10.5061/dryad.nzs7h452g) ## Description of the
 data and file structure Data consist of DNA sequences of nuclear rDNA
 internal and external transcribed spacers from a large number of samples
 of Dieteria collected in the western United States. The sequences were
 subjected to phylogenetic analyses representing many species and varieties
 of Dieteria to investigate their relationships and evolutionary history.
 ### Files and variables #### File:
 MorganDec24\_Dieteria\_data\_matrix\_1.txt **Description:** Combined
 internal and external transcribed spacer DNA sequences from 50 Dieteria
 samples Variables: Nucleotides A, C, G, and T; missing data indicated by
 "N" #### File: MorganDec24\_Dieteria\_data\_matrix\_2.txt
 **Description:** Combined internal and external transcribed spacer DNA
 sequences from 86 Dieteria samples Variables: Nucleotides A, C, G, and T;
 missing data indicated by "N" #### File:
 MorganDec24\_Dieteria\_ETS\_data\_matrix.txt **Description:** External
 transcribed spacer DNA sequences from 69 Dieteria samples Variables:
 Nucleotides A, C, G, and T; missing data indicated by "N" ####
 File: MorganDec24\_Dieteria\_ITS\_data\_matrix.txt
 **Description:** Internal transcribed spacer DNA sequences from 46
 Dieteria samples Variables: Nucleotides A, C, G, and T; missing data
 indicated by "N" ## Code/software All files are unformatted
 text-only files, and can be opened by many types of software, including
 word processors. ## Access information Other publicly accessible locations
 of the data: [https://ncbi.nlm.nih.gov](https://ncbi.nlm.nih.gov);
 accession numbers PQ082737-PQ082832 and PQ094007-PQ094074