cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/5f1fd-q5k94
title: Bdellovibrio2492/E.coli minicell

description: Bdellovibrio HI Strain 2492 in mixture with E. coli WM3433 minicellsGrid box YWC107
description: <strong>Tilt Series Date:</strong> 2014-07-18</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio HI 2492 grown from frozen stocks in liquid PY media at 37C for 7 days, subculturing half of culture into equal volume of fresh media every two days. E. coli minicells WM3433 grown O/N to high OD and spun down 7 mins at 6krpm.  Supernatant spun 10 mins at 14krpm to pellet minicells.  Minicells resuspended in 20ul media and combined in 1:1 v/v ratio with Bdellovibrio.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. Manual blotting, 3 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-9/rawdata/</p>20140718_Janelia_YWC107_10003.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-9/rawdata/20140718_Janelia_YWC107_10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140718_Janelia_YWC107_10003_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-9/3dimage_45093/20140718_Janelia_YWC107_10003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/628c3-3xd81
title: A413T cooverexpression

description: co overexpression strain UU1607/pJC3/pPM25  with A413T mutation in tsr (mutation made by Cathereine, strain and plasmids published. Sent from Heather Hodges (Kiessling lab)
description: <strong>Tilt Series Date:</strong> 2013-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew UU1607/pJC3/pPM25 overnight in 5mL TB + 3.8µl Cm + 5µl CarbDiluted back 1:50 into 200mL TB + 152µl Cm + 200µl Carb + 50µl 1M IPTG + 200µl Na-salicylateGrew ~2 hours to mid-log phaseSpun down 10' at 5,000 x gResuspended in 300µl lysis buffer + 0.1µl PMSF + 0.75 µl 1,10-phenanthrolineAdded 33.3µl (2mg/ml) fresh lysozyme solution (20mg/ml in 10mM Tris-HCl, pH 8.0)Incubated 45' at 37°CAdded 1.67µl 1M MgCl2, 0.2µl 1M CaCl2, 0.33µl DNase IIncubated 30' at 37°CSpun down 4000 g 5 min Resuspended in activity buffer (cold room) without glycerol</p>
  aba2013-08-30-34_slicer9549.jpg: Escherichia coli .  vesicle aba2013-08-30-34_slicer9550.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-34/rawdata/</p>A413tCoover0035.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-34/rawdata/A413tCoover0035.mrc"
        > <i class="download icon"></i> Download </a></p>

        A413tCoover0035_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-08-30-34/3dimage_30379/A413tCoover0035_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/apy5q-j7n05
title: E. coli CusCFBA-CusC-K98H

description: Overexpressed CusCFBA efflux pump in E. coli C43 (DE3).Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-06-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Edward Yu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.</p>
  ycw2013-06-07-7_slicer7864.jpg: Ring .  Escherichia coli ycw2013-06-07-7_slicer7865.jpg: filament .  Escherichia coli ycw2013-06-07-7_slicer7869.jpg: filament .  Escherichia coli ycw2013-06-07-7_slicer7873.jpg: Unknown filament .  DNA Toroid? .  Escherichia coli ycw2013-06-07-7_slicer7879.jpg: pili .  Escherichia coli .  10.2964 nm ycw2013-06-07-7_slicer8005.jpg: pili .  Escherichia coli .  10 nm
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-7/rawdata/</p>CusCFBA_CusCK98H_20004.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-7/rawdata/CusCFBA_CusCK98H_20004.mrc"
        > <i class="download icon"></i> Download </a></p>

        CusCFBA_CusCK98H_20004_part121_9.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-7/3dimage_27235/CusCFBA_CusCK98H_20004_part121_9.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t1mne-05584
title: Vibrio cholerae MKW1383

description: In the mixture with Bdellovibrio for 16 hr at 30C.
description: <strong>Tilt Series Date:</strong> 2013-11-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 16 hr at 30 C before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu R2/2 200 grid</p>
  ycw2013-11-06-1_slicer5944.jpg: Chemoreceptor ycw2013-11-06-1_slicer5946.jpg: unknown filament ycw2013-11-06-1_slicer5947.jpg: Flagellar motor? ycw2013-11-06-1_slicer5949.jpg: Unknown ycw2013-11-06-1_slicer5950.jpg: Unknown ycw2013-11-06-1_slicer5951.jpg: Unknown ycw2013-11-06-1_slicer5952.jpg: Chemoreceptor ycw2013-11-06-1_slicer6036.jpg: unknown coil
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-06-1/rawdata/</p>02-montage_5x50006.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-06-1/rawdata/02-montage_5x50006.mrc"
        > <i class="download icon"></i> Download </a></p>

        02-montage_5x50006_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-06-1/3dimage_34097/02-montage_5x50006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ej8vd-nmg74
title: H. pylori 26695 / AGS

description: Grid box YWC054
description: <strong>Tilt Series Date:</strong> 2013-11-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
  ycw2013-11-25-27_slicer17892.jpg: cag T4SS worm pili ycw2013-11-25-27_slicer17893.jpg: cag T4SS worm pili
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-27/rawdata/</p>20131125_YWC054_10027.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-27/rawdata/20131125_YWC054_10027.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131125_YWC054_10027_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-27/3dimage_35069/20131125_YWC054_10027_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5b7qx-v3t90
title: Pdb 121509 MP35

description: Prosthecobacter debontii, 09dec18a_MP35_II_Pdb_085,
description: <strong>Tilt Series Date:</strong> 2009-12-18</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Prosthecobacter debontii</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
  mpa2009-12-18-60_slicer7900.jpg: Aliens .  Prosthecobacter debontii mpa2009-12-18-60_slicer7903.jpg: Aliens .  Prosthecobacter debontii mpa2009-12-18-60_slicer7904.jpg: Aliens .  Prosthecobacter debontii mpa2009-12-18-60_slicer8049.jpg: Aliens Prosthecobacter debontii mpa2009-12-18-60_slicer8050.jpg: Aliens Prosthecobacter debontii mpa2009-12-18-60_slicer8051.jpg: Aliens Prosthecobacter debontii mpa2009-12-18-60_slicer8053.jpg: Aliens Prosthecobacter debontii mpa2009-12-18-60_slicer8055.jpg: Aliens Prosthecobacter debontii mpa2009-12-18-60_slicer8056.jpg: Aliens Prosthecobacter debontii mpa2009-12-18-60_slicer8058.jpg: Prosthecobacter debontii .  Alien mpa2009-12-18-60_slicer8060.jpg: Prosthecobacter debontii .  Alien mpa2009-12-18-60_slicer8062.jpg: Prosthecobacter debontii .  Alien
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-12-18-60/rawdata/</p>09dec18a_MP35_II_Pdb_085.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-12-18-60/rawdata/09dec18a_MP35_II_Pdb_085.mrc"
        > <i class="download icon"></i> Download </a></p>

        09dec18a_MP35_II_Pdb_085_part.rec,  Reconstruction (Pixel Size 1.9 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-12-18-60/3dimage_14355/09dec18a_MP35_II_Pdb_085_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.34894/6ohrod
title: Neural mechanisms underlying trust to friends, community members, and unknown peers in adolescence (Tasks and questions)

description: &lt;p&gt;This item contains the tasks and questions for Sweijen et al (2023). Neural mechanisms underlying trust to friends, community members, and unknown peers in adolescence. Journal of Cognitive Neuroscience.&lt;/p&gt;
&lt;p&gt;Trust plays an important role during adolescence for developing social relations. While prior developmental studies give us insight into adolescents’ development of differentiation between close (e.g., friends) and unknown (e.g., unknown peers) targets in trust choices, less is known about the development of trust to societal targets (e.g., members of a community organization), and its underlying neural mechanisms. Using a modified version of the Trust Game, our preregistered fMRI study examined the underlying neural mechanisms of trust to close (friend), societal (community member), and unknown others (unknown peer) during adolescence in 106 participants (aged 12-23). Adolescents showed most trust to friends, less trust to community members, and the least trust to unknown peers. Neural results show that target differentiation in adolescents’ trust behavior is associated with activity in social brain regions implicated during mentalizing, reward processing, and cognitive control. Recruitment of the medial prefrontal cortex (mPFC) and orbitofrontal cortex was higher for closer targets (i.e., friend and community member). For the mPFC, this effect was most pronounced during no trust choices. Trust to friends was additionally associated with increased activity in the precuneus and bilateral temporal parietal junction. In contrast, bilateral dorsolateral prefrontal cortex and anterior cingulate cortex were most active for trust to unknown peers. The mPFC showed increased activity with age and consistent relations with individual differences in feeling needed/useful.&lt;/p&gt;
&lt;p&gt;This publication package contains the tasks and questions used in the study. In this study, a modified version of the Trust Game was used, which we introduced to participants as the ‘Color game’ (in Dutch: ‘kleurenspel’) to avoid influencing participants’ trust choices.&lt;/p&gt;

&lt;p&gt;
&lt;b&gt;Embargo Justification:&lt;/b&gt; This publication package contains the tasks and questions, which are confidential. Files are accessible to the authors of the study. Interested parties can request access by e-mailing sweijen@essb.eur.nl. When requesting data, please explain why you would like to access the files.
&lt;/p&gt;
description: This entry is a twenty-two-file data package totaling 72.4 MB, containing files in .ebs3, .es3, .png, .mp4, .wndpos, .pptx and .docx formats.If you use this dataset, please cite: Sweijen, Sophie; van de Groep, Suzanne; te Brinke, Lysanne; Fuligni, Andrew; Crone, Eveline A. (2023). Neural mechanisms underlying trust to friends, community members, and unknown peers in adolescence (Tasks and questions). Erasmus University Rotterdam (EUR). Dataset. https://doi.org/10.25397/eur.24050028
DOI: 10.15468/dl.dhv9js
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Mexico",
 "ScientificName is Hypothenemus eruditus"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006214-250827131500795/datasets/export for details.
DOI: 10.22002/3qd5z-v5354
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC065
description: <strong>Tilt Series Date:</strong> 2014-05-12</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2014-05-12-26_slicer3905.jpg: Myxococcus xanthus delta-tsaP. Cross-section of a big filament bundle. ycw2014-05-12-26_slicer3906.jpg: Myxococcus xanthus delta-tsaP. Big filament bundle. ycw2014-05-12-26_slicer7647.jpg: filament bundle in Myxococcus xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-26/3dimage_42428/</p>20140512_YWC065_40007_part121_2.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-26/3dimage_42428/20140512_YWC065_40007_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        20140512_YWC065_40007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-26/rawdata/20140512_YWC065_40007.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7d8ye-t2043
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-27/rawdata/</p>20140331_YWC097_10027.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-27/rawdata/20140331_YWC097_10027.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10027_full.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-27/3dimage_40672/20140331_YWC097_10027_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nxnqq-vm340
title: H.pylori 26695 delta-PAI / AGS

description: Grid box: YWC098
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-25/rawdata/</p>20140401_YWC098_10025.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.3 GB  
        
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        20140401_YWC098_10025_full.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
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