DOI: 10.5061/dryad.70rxwdbxm
title: Dataset for Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors

description: Use of normothermic ex vivo lung perfusion (EVLP) was adopted in clinical
 practice to assess the quality of marginal donor lungs. Subnormothermic
 perfusion temperatures are in use among other solid organs to improve
 biochemical, clinical and immunological parameters. In a rat EVLP model of
 donation after circulatory death (DCD) lung donors, we tested the effect
 of four subnormothermic EVLP temperatures that could further improve organ
 preservation. Warm ischemic time was of 2 hours. EVLP time was of 4 hours.
 Lung physiological data were recorded and metabolic parameters were
 assessed. Lung oxygenation at 21°C and 24°C were significantly improved
 whereas pulmonary vascular resistance and edema formation at 21°C EVLP
 were significantly worsened when compared to 37°C EVLP. The perfusate
 concentrations of potassium ions and lactate exiting the lungs with 28°C
 EVLP were significantly lower whereas sodium and chlorine ions with 32°C
 EVLP were significantly higher when compared to 37°C EVLP. Also compared
 to 37°C EVLP, the pro-inflammatory chemokines MIP2, MIP-1α, GRO-α, the
 cytokine IL-6 were significantly lower with 21°C, 24°C and 28°C EVLP, the
 IL-18 was significantly lower but only with 21°C EVLP and IL-1β was
 significantly lower at 21°C and 24°C EVLP. Compared to the 37°C EVLP, the
 lung tissue ATP content after 21°C, 24°C and 28°C EVLP were significantly
 higher, the carbonylated protein content after 28°C EVLP was significantly
 lower and we measured significantly higher myeloperoxidase activities in
 lung tissues with 21°C, 24°C and 32°C. The 28°C EVLP demonstrated
 acceptable physiological variables, significantly higher lung tissue ATP
 content and decreased tissue carbonylated proteins with reduced release of
 pro-inflammatory cytokines. In conclusion, the 28°C EVLP is a non inferior
 setting in comparison to the clinically approved 37°C EVLP and
 significantly improve biochemical, clinical and immunological parameters
 and may reduce I/R injuries of DCD lung donors.
description: This Readme text Dataset for Subnormothermic ex vivo lung.txt
 file was generated on 20210717 by Stephan Arni GENERAL
 INFORMATION 1. Title of Dataset:  Dataset for
 Subnormothermic ex vivo lung 2. Author
 Information<br>     A. Principal Investigator Contact
 Information<br>         Name: Ilhan Inci.<br>       
  Institution: Department of Thoracic Surgery, University Hospital Zurich,
 Zurich, Switzerland.<br>         Address: Rämistrasse 100 8091
 Zurich, Switzerland.<br>         Email: Ilhan.inci@usz.ch
     B. Associate or Co-investigator Contact Information<br>
         Name: Stephan Arni<br>         Institution: Department of
 Thoracic Surgery, University Hospital Zurich, Zurich,
 Switzerland.<br>         Address: Rämistrasse 100 8091 Zurich,
 Switzerland.<br>         Email: Stephan.arni@usz.ch
 3. Date of data collection: 20171113 to 20180517.
 4. Geographic location of data collection: irrelevant.
 5. Information about funding sources that supported the
 collection of the data: This study was founded by the Swiss Lung
 Foundation, the Fondation A.P. Naef, the Jubi-läumsstiftung der Lungen
 Liga Graubünden. SHARING/ACCESS INFORMATION
 1. Licenses/restrictions placed on the data: none
 2. Links to publications that cite or use the data:
 Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion
 injury from donation after circulatory death donors
 https://doi:10.1371/journal.pone.0255155   3. Links to
 other publicly accessible locations of the data: none.
 4. Links/relationships to ancillary data sets:
 https://datadryad.org/stash/share/8qGUI1W6S5h3aba4t5HVXQTdmneQ2v-xnBSBmbB37A0   5. Was data derived from another source? no 6. Recommended citation for this dataset: Arni, S.; Maeyashiki, T.; Opitz, I.; Inci, I. Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors. PLoS One 2021, 10.1371/journal.pone.0255155, Accepted. In press.  DATA & FILE OVERVIEW 1. File List: <br> Figure_1.csv Are all the data of the figure 1 Physiological variables and oxygenation parameters.<br> Figure_2.csv Are all the data of the figure 2 Clinical biochemistry parameters.<br> Figure_3.csv Are all the data of the figure 3 Cytokines and chemokines in the perfusate.<br> Figure_4.csv Are all the data of the figure 4 TUNEL assay, Myeloperoxidase activity, ATP and carbonylated protein content in lung tissues.<br> 2. Relationship between files, if important: Data files are independent from each other.<br> 3. Additional related data collected that was not included in the current data package: none<br> 4. Are there multiple versions of the dataset? no<br>     A. If yes, name of file(s) that was updated: <br>         i. Why was the file updated? <br>         ii. When was the file updated?  METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: <br> Arni, S.; Maeyashiki, T.; Opitz, I.; Inci, I. Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors. PLoS One 2021, 10.1371/journal.pone.0255155, Accepted. In press. 2. Methods for processing the data: <br> Arni, S.; Maeyashiki, T.; Opitz, I.; Inci, I. Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors. PLoS One 2021, 10.1371/journal.pone.0255155, Accepted. In press. 3. Instrument- or software-specific information needed to interpret the data: <br> Graph pad prism V8.0. 4. Standards and calibration information, if appropriate: none 5. Environmental/experimental conditions: irrelevant 6. Describe any quality-assurance procedures performed on the data: raw data  7. People involved with sample collection, processing, analysis and/or submission: Stephan Arni DATA-SPECIFIC INFORMATION FOR: Figure_1.csv<br> 1. Number of variables: 4<br> 2. Number of cases/rows: 26 cases <br> 3. Variable List: <br> Figure 1A Pulmonary vascular resistance (cmH2O/ml/min)<br> Figure 1B Dynamic compliance (ml/cmH2O)<br> Figure 1C oxygenation delta O2 (mmHg)<br> Figure 1D Lung percent weight gain/loss (%)<br> 4. Missing data codes: none<br> 5. Specialized formats or other abbreviations used: none DATA-SPECIFIC INFORMATION FOR: Figure_2.csv<br> 1. Number of variables: 6<br> 2. Number of cases/rows: 26 cases <br> 3. Variable List: <br> Figure 2A Potassium from pulmonary vein (mmol/L)<br> Figure 2B Lactate from pulmonary vein (mmol/L)<br> Figure 2C Calcium from pulmonary vein (mmol/L)<br> Figure 2D Glucose from pulmonary vein (mg/dL)<br> Figure 2E Sodium from pulmonary vein (mmol/L)<br> Figure 2F Chlorine from pulmonary vein (mmol/L)<br> 4. Missing data codes: none<br> 5. Specialized formats or other abbreviations used: none DATA-SPECIFIC INFORMATION FOR: Figure_3.csv<br> 1. Number of variables: 9<br> 2. Number of cases/rows: 26 cases <br> 3. Variable List: <br> Figure 3A MIP1 alpha (pg/mL)<br> Figure 3B MIP2 (pg/mL)<br> Figure 3C GRO alpha (pg/mL))<br> Figure 3D IL6 (pg/mL)<br> Figure 3E IL18 (pg/mL)<br> Figure 3F IL1Beta (pg/mL)<br> Figure 3G IL1alpha (pg/mL)<br> Figure 3H MCP1 (pg/mL)<br> Figure 3I VEGF (pg/mL)<br> 4. Missing data codes: none<br> 5. Specialized formats or other abbreviations used: none DATA-SPECIFIC INFORMATION FOR: Figure_4.csv<br> 1. Number of variables: 4<br> 2. Number of cases/rows: 26 cases <br> 3. Variable List: <br> Figure 4A Tissue ATP (nmol/mg protein)<br> Figure 4B Tissue MPO (microUl/mg protein)<br> Figure 4C Tissue carbonyl proteins (nmol/mL)<br> Figure 4D Tunel positive cells (average number of 10 fields per slide)<br> 4. Missing data codes: none<br> 5. Specialized formats or other abbreviations used: none