cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.23584943
title: Development of inclusion complex based on cyclodextrin and oxazolidine derivative

description: Abstract Oxazolidine derivatives (OxD) have been described as third-line antibiotics and antitumoral agents. The inclusion complexes based on cyclodextrin could improve the solubility and bioavailability of these compounds. A novel synthetic OxD was used, and its inclusion complexes were based on 2-hydroxy-beta-cyclodextrin (2-HPβCD). We conducted an in silico study to evaluate the interaction capacity between OxD and 2-HPβCD. Characterization studies were performed through scanning electron microscopy (SEM), Fourier-transformed infrared (FTIR), nuclear magnetic resonance spectroscopy (1H-NMR), X-ray diffraction (XRD), and thermal analyses. A kinetic study of the OxD was performed, including a cytotoxicity assay using peripheral blood mononuclear cells (PBMCs). The maximum increment of solubility was obtained at 70 mM OxD using 400 mM 2-HPβCD. SEM analyses and FTIR spectra indicated the formation of inclusion complexes. 1H-NMR presented chemical shifts that indicated 1:1 stoichiometry. Different thermal behaviors were obtained. The pharmacokinetic profile showed a short release time. Pure OxD and its inclusion complex did not exhibit cytotoxicity in PBMCs. In silico studies provided a foremost insight into the interactions between OxD and 2-HPβCD, including a higher solubility in water and an average releasing profile without toxicity in normal cells.
DOI: 10.6084/m9.figshare.23584943.v1
title: Development of inclusion complex based on cyclodextrin and oxazolidine derivative

description: Abstract Oxazolidine derivatives (OxD) have been described as third-line antibiotics and antitumoral agents. The inclusion complexes based on cyclodextrin could improve the solubility and bioavailability of these compounds. A novel synthetic OxD was used, and its inclusion complexes were based on 2-hydroxy-beta-cyclodextrin (2-HPβCD). We conducted an in silico study to evaluate the interaction capacity between OxD and 2-HPβCD. Characterization studies were performed through scanning electron microscopy (SEM), Fourier-transformed infrared (FTIR), nuclear magnetic resonance spectroscopy (1H-NMR), X-ray diffraction (XRD), and thermal analyses. A kinetic study of the OxD was performed, including a cytotoxicity assay using peripheral blood mononuclear cells (PBMCs). The maximum increment of solubility was obtained at 70 mM OxD using 400 mM 2-HPβCD. SEM analyses and FTIR spectra indicated the formation of inclusion complexes. 1H-NMR presented chemical shifts that indicated 1:1 stoichiometry. Different thermal behaviors were obtained. The pharmacokinetic profile showed a short release time. Pure OxD and its inclusion complex did not exhibit cytotoxicity in PBMCs. In silico studies provided a foremost insight into the interactions between OxD and 2-HPβCD, including a higher solubility in water and an average releasing profile without toxicity in normal cells.
DOI: 10.6084/m9.figshare.23584944
title: URBAN GRADIENT

description: Abstract As in many other countries, official measures of urbanization in Brazil have been unable to properly distinguish spatial patterns of rural and urban organization. The literature discusses various classification systems and recognizes the existence of a “rural-urban continuum”, as well as theoretical frameworks and alternative categorizations that aim to look beyond the urban-rural dichotomy. However, there are no practical solutions to properly formalize or operationalize them, especially at the micro-level such as census tracts. We propose a methodology that assumes a measurable rural-urban continuum. The “Urban Gradient Index” uses simple and comprehensive variables to quantify the degree to which a certain area is urban based on demographic and spatial features. We build it at the level of census tracts for both Brazil and the Belo Horizonte Metropolitan Region to verify consistency at distinct aggregation levels. Results indicate that the index provides a much more nuanced picture of settlement patterns, unveiling a spatial gradient between rural and urban in distinct spatial extents. It offers an advantage over the traditional measure “degree of urbanization” by revealing “hidden ruralities” in predominantly urban areas that require specific territorial planning and public policy interventions.
DOI: 10.6084/m9.figshare.23584944.v1
title: URBAN GRADIENT

description: Abstract As in many other countries, official measures of urbanization in Brazil have been unable to properly distinguish spatial patterns of rural and urban organization. The literature discusses various classification systems and recognizes the existence of a “rural-urban continuum”, as well as theoretical frameworks and alternative categorizations that aim to look beyond the urban-rural dichotomy. However, there are no practical solutions to properly formalize or operationalize them, especially at the micro-level such as census tracts. We propose a methodology that assumes a measurable rural-urban continuum. The “Urban Gradient Index” uses simple and comprehensive variables to quantify the degree to which a certain area is urban based on demographic and spatial features. We build it at the level of census tracts for both Brazil and the Belo Horizonte Metropolitan Region to verify consistency at distinct aggregation levels. Results indicate that the index provides a much more nuanced picture of settlement patterns, unveiling a spatial gradient between rural and urban in distinct spatial extents. It offers an advantage over the traditional measure “degree of urbanization” by revealing “hidden ruralities” in predominantly urban areas that require specific territorial planning and public policy interventions.
DOI: 10.6084/m9.figshare.23584946
title: Medical preceptorship: perceptions and perspectives of new family doctors in Brazil

description: Abstract: Introduction: Learning in practical environments consists in an essential component of medical education. In recent decades, medical schools have sought to immerse their students in professional settings since the early years of studying. In these scenarios, the preceptors, more experienced physicians, are responsible for supporting their students in the development of professional knowledge, skills, and attitudes. Objective: This study aimed to analyze the perceptions and perspectives of participants of the Preceptorship Training Course (PTC) in Family Medicine - UNA-SUS about preceptorship and medical education. Methods: The quantitative descriptive study was conducted in two phases: ‘Population Outlining’ phase, including participants’ academic data, and ‘Survey Questionnaire’ phase, performed by applying a questionnaire through a digital platform. Results: The ‘Population Outlining’ identified 2,530 participants in the PTC, with the predominance of females and southeastern residents. The ‘Survey Questionnaire’ phase constituted of 232 respondents, of whom 73.4% reported acknowledging their preceptors’ contributions, and more than 90% expressed interest in teaching activities, valuing their curriculum enhancement and academic acknowledge. Conclusion: Our findings evidenced positive perceptions about preceptorship and a propensity to engage in teaching activities among the participants, reinforcing the importance of debates about qualification, recruiting and retention of preceptors.
DOI: 10.6084/m9.figshare.23584946.v1
title: Medical preceptorship: perceptions and perspectives of new family doctors in Brazil

description: Abstract: Introduction: Learning in practical environments consists in an essential component of medical education. In recent decades, medical schools have sought to immerse their students in professional settings since the early years of studying. In these scenarios, the preceptors, more experienced physicians, are responsible for supporting their students in the development of professional knowledge, skills, and attitudes. Objective: This study aimed to analyze the perceptions and perspectives of participants of the Preceptorship Training Course (PTC) in Family Medicine - UNA-SUS about preceptorship and medical education. Methods: The quantitative descriptive study was conducted in two phases: ‘Population Outlining’ phase, including participants’ academic data, and ‘Survey Questionnaire’ phase, performed by applying a questionnaire through a digital platform. Results: The ‘Population Outlining’ identified 2,530 participants in the PTC, with the predominance of females and southeastern residents. The ‘Survey Questionnaire’ phase constituted of 232 respondents, of whom 73.4% reported acknowledging their preceptors’ contributions, and more than 90% expressed interest in teaching activities, valuing their curriculum enhancement and academic acknowledge. Conclusion: Our findings evidenced positive perceptions about preceptorship and a propensity to engage in teaching activities among the participants, reinforcing the importance of debates about qualification, recruiting and retention of preceptors.
DOI: 10.6084/m9.figshare.23584887
title: The diversity and political bias of personalized news

description: This data is a measurement of variables on the diversity and political bias of Internet portal news.
DOI: 10.6084/m9.figshare.23584952
title: Effects of Chitosan Nanoparticles with Long Synthetic siRNAs Targeting VEGF in Triple-Negative Breast Cancer Cells

description: Abstract Vascular endothelial growth factor (VEGF) is an essential angiogenic factor in breast cancer development and metastasis. Small interfering RNAs (siRNAs) can specifically silence genes via the RNA interference pathway, therefore were investigated as cancer therapeutics. In this study, we investigated the effects of siRNAs longer than 30 base pairs (bp) loaded into chitosan nanoparticles in triple-negative breast cancer cells, compared with conventional siRNAs. 35 bp long synthetic siRNAs inhibited VEGF gene expression by 51.2% and increased apoptosis level by 1.75-fold in MDA-MB-231 cell lines. Furthermore, blank and siRNA-loaded chitosan nanoparticles induced expression of IFN-γ in breast cancer cells. These results suggest that long synthetic siRNAs can be as effective as conventional siRNAs, when introduced into cells with chitosan nanoparticles.
DOI: 10.6084/m9.figshare.23584952.v1
title: Effects of Chitosan Nanoparticles with Long Synthetic siRNAs Targeting VEGF in Triple-Negative Breast Cancer Cells

description: Abstract Vascular endothelial growth factor (VEGF) is an essential angiogenic factor in breast cancer development and metastasis. Small interfering RNAs (siRNAs) can specifically silence genes via the RNA interference pathway, therefore were investigated as cancer therapeutics. In this study, we investigated the effects of siRNAs longer than 30 base pairs (bp) loaded into chitosan nanoparticles in triple-negative breast cancer cells, compared with conventional siRNAs. 35 bp long synthetic siRNAs inhibited VEGF gene expression by 51.2% and increased apoptosis level by 1.75-fold in MDA-MB-231 cell lines. Furthermore, blank and siRNA-loaded chitosan nanoparticles induced expression of IFN-γ in breast cancer cells. These results suggest that long synthetic siRNAs can be as effective as conventional siRNAs, when introduced into cells with chitosan nanoparticles.
DOI: 10.6084/m9.figshare.23584955
title: Simultaneous Estimation and Validation of Four Antiepileptic Drugs from Bulk and Formulations Using Reverse Phase HPLC

description: Abstract Epilepsy is a disorder of the central nervous system, in which the nerve cell activity in the brain is disturbed causing seizures. The objective was to develop an RP-HPLC method for consistent simultaneous quantitation of four antiepileptic drugs Levetiracetam (LVT), Lamotrigine (LTG), Phenobarbital (PBT) and Phenytoin (PTY). An isocratic method was developed on C18 column in JASCO HPLC using 5 mM potassium phosphate buffer (pH 6) and acetonitrile as the mobile phase at a flow rate of 1ml/min and detected at 230 nm using UV detector. The mean retention time for LVT, LTG, PBT and PTY were found as 2.55, 3.55, 4.65 and 5.99 minutes respectively. The method was validated as per ICH guidelines and was found to be acceptable. The %RSD value was &lt;2.0 % thus stating the developed method was precise for the drugs in the given range. The accuracy values were within 85-115% of the recovery range. The specificity of the method was evaluated by an assay of marketed formulation, and it showed a percent content between 90-110% w/w for all the four drugs. The proposed analytical method was simple, accurate and robust and was precisely able to resolve the four major antiepileptic drugs. Hence, the current method can be applied successfully for routine examination of these drugs.
DOI: 10.6084/m9.figshare.23584955.v1
title: Simultaneous Estimation and Validation of Four Antiepileptic Drugs from Bulk and Formulations Using Reverse Phase HPLC

description: Abstract Epilepsy is a disorder of the central nervous system, in which the nerve cell activity in the brain is disturbed causing seizures. The objective was to develop an RP-HPLC method for consistent simultaneous quantitation of four antiepileptic drugs Levetiracetam (LVT), Lamotrigine (LTG), Phenobarbital (PBT) and Phenytoin (PTY). An isocratic method was developed on C18 column in JASCO HPLC using 5 mM potassium phosphate buffer (pH 6) and acetonitrile as the mobile phase at a flow rate of 1ml/min and detected at 230 nm using UV detector. The mean retention time for LVT, LTG, PBT and PTY were found as 2.55, 3.55, 4.65 and 5.99 minutes respectively. The method was validated as per ICH guidelines and was found to be acceptable. The %RSD value was &lt;2.0 % thus stating the developed method was precise for the drugs in the given range. The accuracy values were within 85-115% of the recovery range. The specificity of the method was evaluated by an assay of marketed formulation, and it showed a percent content between 90-110% w/w for all the four drugs. The proposed analytical method was simple, accurate and robust and was precisely able to resolve the four major antiepileptic drugs. Hence, the current method can be applied successfully for routine examination of these drugs.
DOI: 10.6084/m9.figshare.23584958
title: ANALYSIS OF THE TOURIST TERRITORIES OF THE PIAUÍ COAST USING THE ANALYTICAL HIERARCHY PROCESS

description: Abstract The article applies the Analytic Hierarchy Process (AHP) to evaluate tourism development on the coast of Piauí, Brazil. The assessment of the tourism territories identified on the coast of Piauí was viable due to a quantitative analysis of the region's tourism activity. By quantifying the importance of the variables "means of accommodation," "food services," "tourist attractions," and "access routes" related to the criteria of "cost," "availability," and "quality," it was possible to evaluate each territory. The negative impacts caused by problems related to infrastructure were evident, especially the difficulty of access. AHP proves to be a methodology with the potential to be incorporated into the planning and management process for tourism, as it is effective in characterizing and evaluating territories used by tourism. This study is expected to support the decision-making of municipal and state public authorities concerning the social and environmental impacts of tourism activity on the coast of Piauí, mainly in fragmented territories.
DOI: 10.6084/m9.figshare.23584958.v1
title: ANALYSIS OF THE TOURIST TERRITORIES OF THE PIAUÍ COAST USING THE ANALYTICAL HIERARCHY PROCESS

description: Abstract The article applies the Analytic Hierarchy Process (AHP) to evaluate tourism development on the coast of Piauí, Brazil. The assessment of the tourism territories identified on the coast of Piauí was viable due to a quantitative analysis of the region's tourism activity. By quantifying the importance of the variables "means of accommodation," "food services," "tourist attractions," and "access routes" related to the criteria of "cost," "availability," and "quality," it was possible to evaluate each territory. The negative impacts caused by problems related to infrastructure were evident, especially the difficulty of access. AHP proves to be a methodology with the potential to be incorporated into the planning and management process for tourism, as it is effective in characterizing and evaluating territories used by tourism. This study is expected to support the decision-making of municipal and state public authorities concerning the social and environmental impacts of tourism activity on the coast of Piauí, mainly in fragmented territories.
DOI: 10.25919/t5v7-yw46
title: Factors affecting canola establishment in Australia

description: This report summarises three activities to identify current best management practices for canola establishment across Australia and knowledge gaps and industry priorities for further research. 1. A literature review was conducted on management and environmental factors affecting canola establishment. This built upon existing reviews but included grey literature such as on-farm trials not normally included in formal literature reviews. The main research opportunities identified were (i) improved understanding of how moisture and temperature interact to reduce establishment when sowing early; (ii) the impact of sowing depth on canola establishment and the ability to “moisture-seek” with deeper sowing; (iii) the impact of crop residue/stubble on establishment under early sowing; (iv) the effect of soil crusting. 2. An online industry survey was carried out between 9 Feb and 28 March 2023. In total there were 126 respondents across the key canola growing regions in Australia. The results showed that farmers generally aimed for 20-40 plants/m2 and were achieving estimated field establishment rates of 60-80%. The issues contributing to poor establishment were: (i) marginal soil moisture, (ii) seeding depth and furrow infill, (iii) surface crusting and (iv) stubble/residue issues. A range of establishment pests were also identified as currently reducing establishment. In terms of farmer and consultant research priorities the most mentioned keywords were ‘depth’, ‘hypocotyl’, ‘moisture’, ‘stubble’ and ‘vigour’. 3. Direct industry consultation was sub-contracted to ‘AgInnovate’. Initially we planned farmer/consultant workshops but found limited interest. Instead AgInnovate contacted grower groups directly and interviewed them. The key conclusions were that the nature of the seasonal break (timing, and amount of rainfall) is a main contributor to establishment success of canola and seed survival in marginal breaks was a research priority; Vic, NSW and SA groups said their establishment rates were &lt; 50-60%; Queensland growers wanted the research to provide answers regarding fungicide applications and seeding depths. Clearly the consistent themes emerging from these activities regarding poor canola establishment and areas to focus further research were: • Optimum sowing date and sowing decisions. Information and tools are required to make decisions around early sowing with respect to soil moisture and temperature thresholds. • More information on optimum sowing depths under different conditions is required, particularly in the context of early seeding. • The impact of stubble on moisture retention, temperature and canola establishment should be re-examined. • The impact of factors leading to soil crusting and contributing factors needs more research.
DOI: 10.6084/m9.figshare.23584968
title: Pre-and postpartum supplementation strategies on the performance and metabolic status of grazing beef cows

description: Abstract The objective of this work was to evaluate the effect of energy-protein supplementation in preand postpartum periods on the productive and metabolic responses of grazing beef cows on tropical pasture. A group of 48 multiparous Nellore cows, with an initial gestation period of 6.4 months, body weight (BW) of 514.9 kg, and body condition score (BCS) of 5.4, was distributed in a completely randomized design, in a 2×2 factorial arrangement. The evaluated treatments were: UNS-UNS, unsupplemented during prepartum and postpartum; UNS-SUP, unsupplemented during prepartum and supplemented during postpartum; SUP-UNS, supplemented during prepartum and unsupplemented during postpartum; and SUP-SUP, supplemented during preand postpartum. The energy-protein supplement was offered at an amount of 1.5 kg per animal per day. Prepartum supplementation increased average daily gain and BCS at calving. Prepartum supplementation reduced non-esterified fatty acids (NEFA) and increased serum concentrations of total proteins and globulins during postpartum. Postpartum supplementation increased the BCS of cows and BW of calves at the end of the experiment. Postpartum supplementation increased blood concentrations of progesterone, while NEFA decreased. Supplementation with 1.5 kg per day of the energy-protein supplement during the last 84 days of gestation improves productive performance and metabolic status during the preand postpartum periods of grazing cows.
DOI: 10.6084/m9.figshare.23584968.v1
title: Pre-and postpartum supplementation strategies on the performance and metabolic status of grazing beef cows

description: Abstract The objective of this work was to evaluate the effect of energy-protein supplementation in preand postpartum periods on the productive and metabolic responses of grazing beef cows on tropical pasture. A group of 48 multiparous Nellore cows, with an initial gestation period of 6.4 months, body weight (BW) of 514.9 kg, and body condition score (BCS) of 5.4, was distributed in a completely randomized design, in a 2×2 factorial arrangement. The evaluated treatments were: UNS-UNS, unsupplemented during prepartum and postpartum; UNS-SUP, unsupplemented during prepartum and supplemented during postpartum; SUP-UNS, supplemented during prepartum and unsupplemented during postpartum; and SUP-SUP, supplemented during preand postpartum. The energy-protein supplement was offered at an amount of 1.5 kg per animal per day. Prepartum supplementation increased average daily gain and BCS at calving. Prepartum supplementation reduced non-esterified fatty acids (NEFA) and increased serum concentrations of total proteins and globulins during postpartum. Postpartum supplementation increased the BCS of cows and BW of calves at the end of the experiment. Postpartum supplementation increased blood concentrations of progesterone, while NEFA decreased. Supplementation with 1.5 kg per day of the energy-protein supplement during the last 84 days of gestation improves productive performance and metabolic status during the preand postpartum periods of grazing cows.