cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/tch37-fgk51
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-65/rawdata/</p>64.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-65/rawdata/64.mrc"
        > <i class="download icon"></i> Download </a></p>

        64_part122_12.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-65/3dimage_24443/64_part122_12.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hgk9q-2qw78
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-32/rawdata/</p>33.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-32/rawdata/33.mrc"
        > <i class="download icon"></i> Download </a></p>

        33_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-32/3dimage_24408/33_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2nfvh-49m42
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-12/rawdata/</p>12.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-12/rawdata/12.mrc"
        > <i class="download icon"></i> Download </a></p>

        12_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-12/3dimage_24387/12_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vtezf-gdy59
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-28/rawdata/</p>2.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-28/rawdata/2.mrc"
        > <i class="download icon"></i> Download </a></p>

        2_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-28/3dimage_24404/2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xn0hd-nz948
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-5/rawdata/</p>73.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-5/rawdata/73.mrc"
        > <i class="download icon"></i> Download </a></p>

        73_part122_42.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-5/3dimage_24386/73_part122_42.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/thyg9-a2g22
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-69/rawdata/</p>68.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-69/rawdata/68.mrc"
        > <i class="download icon"></i> Download </a></p>

        68_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-69/3dimage_24445/68_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4cf7v-4em61
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-35/rawdata/</p>36.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-35/rawdata/36.mrc"
        > <i class="download icon"></i> Download </a></p>

        36_part122_11.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-35/3dimage_24417/36_part122_11.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kqmr8-qt591
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-62/rawdata/</p>61.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-62/rawdata/61.mrc"
        > <i class="download icon"></i> Download </a></p>

        61_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-62/3dimage_24439/61_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/062q1-bdy13
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-55/rawdata/</p>54.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-55/rawdata/54.mrc"
        > <i class="download icon"></i> Download </a></p>

        54_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-55/3dimage_24436/54_full.rec"
        > <i class="download icon"></i> Download </a></p>