cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.279
title: Sensitivity in error detection of patient-specific QA tools for IMRT plans

description: Currently, there are many patient specific QA tools that are available in the commercial. The configuration of each device and their efficiencies are also difference from each other. Thus the patient specific QA tools that used in the clinical fields need to be chosen according to their efficiency and daily routine needs. The purpose of this study is to investigate the error detection capability of the portal dosimetry system, MapCHECK 2 system and MatriXX system in IMRT plans. These three devices used for the 2D patient specific QA tools in this study. The 4 head and neck plans (the complicated plan) and 4 prostate plans (the simple plan) were studied for error detection of QA devices. The 6 MV beams were employed with 9 field arrangement for head and neck IMRT cases while 10 MV beams with 7 fields were optimized and calculated for prostate IMRT plans. Measurements were undertaken for the original plan and the modified plans with errors introduced in order to check the sensitivity of the QA tools system. The intentional errors composed of increasing and decreasing of prescribe dose (±2, ±4 and ±6%) and position shifting in X-axis and Y-axis ( ±1, ±2, ±3 and ±5 mm) both representing the setup uncertainty. After measurement, the results were compared between calculated and measured values of the original plan using gamma analysis at 3%/3mm criteria. Then the gamma pass between original and the intentional error were compared. The average gamma pass of original head and neck plans analyzed by 3%/3mm were 96.9, 98.6 and 98.8 for portal dosimetry, MapCHECK 2 system and MatirXX system, respectively. The average gamma pass for prostate plans were 99.4, 99.0 and 99.7 for portal dosimetry, MapCHECK 2 system and MatirXX system, respectively. In head and neck plans, the shift error detections were 1mm for portal dosimetry, 2mm for MapCHECK 2 and 3mm for MatriXX system, respectively. In prostate plan, the shift error detections were 2mm for portal dosimetry, 3mm for MapCHECK 2 and 5mm for MatriXX system, respectively. For the dose error detection, the portal dosimetry could detect 2% dose in head and neck plan and 4% dose in prostate plan. The other two devices could detect 4% dose in both head and neck and prostate plans. The error detection depends on the detector resolution and the type of plan. If the QA device has higher resolution, it can have more sensitivity to small error detection than less resolution detector.
DOI: 10.13145/bacdive137641.20191129.4.1
title: Erysipelothrix rhusiopathiae

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive137642.20191129.4.1
title: Lactobacillus acidophilus

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.280
title: Lactate non-clearance versus lactate clearance : a comparison of hospital mortality in high-risk surgical patients

description: Objective: The optimal endpoints of resuscitation in high-risk surgical patients remain controversial. Specifically, it is difficult to establish the effective predictive markers as the endpoints of resuscitation in this patient group. Therefore, the study was conducted to assess the predictive value of early lactate non-clearance condition on hospital mortality in high-risk surgical patients. Materials and Methods: The study is a prospective analytic study. The data was collected in one university-based surgical intensive care unit (SICU) over a 5-month period. All consecutive adult high-risk surgical patients admitted to SICU in postoperative period were recruited to the study. Blood lactate levels were measured on SICU admission (0-hour), 12 hours later, and then calculated for 12-hour blood lactate clearance. The authors categorized the patients into two groups: lactate clearance (LC) and lactate non-clearance (LNC). After that, the patients were monitored until hospital discharge or in-hospital death. Results: 122 high-risk surgical patients were recruited to the study. The most common indication for SICU admission was extensive ablative surgery for carcinoma. As concerns the factors of interest, higher incidences of suspected or confirmed infection and mechanical ventilation were found among the LNC group. Regarding the main outcomes, hospital mortality was 5.3% among the LNC group and 3.9% among the LC group (p = 0.578), with no statistical significant differences in hospital mortality, hospital length of stay and SICU length of stay. The independent risk factors associated with LNC condition were considered. The factor of interest was suspected or confirmed infection by multiple logistic regression analysis after adjustment for age and sex revealed that the adjusted odds ratio was 2.70 with a 95% confidence interval of 0.85-8.55, p = 0.092. Conclusion: 12-hour LNC in high-risk surgical patients cannot demonstrate the prognostic value for hospital morbidity and mortality. However, there is a trend for the suspected or confirmed infection group to associate with the LNC condition but with no statistical significance.
DOI: 10.13145/bacdive137643.20191129.4.1
title: Acinetobacter baumannii

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.281
title: Development of multiplex PCR for diagnosis of scrub typhus, rickettsial disease and leptospirosis

description: Scrub typhus, leptospirosis, and murine typhus are life-threatening zoonosis worldwide. The causative agent of these diseases is Orientia tsutsugamushi, Leptospira interrogans, and Rickettsia typhi, respectively. Transmission of these bacteria occurs through vector biting or directly bacteria contraction. Clinical features of these diseases are as acute undifferentiated fever such as high fever, headache, muscular pain, and anorexia, resulting to delay diagnosis and mortality. Although a serological laboratory test is gold standard for scrub typhus, leptospirosis, and murine typhus diagnosis, it has own limitation because inadequate antibody in the early phase. In this study, we aimed to develop the multiplex PCR on the causative agents of scrub typhus, leptospirosis, and murine typhus detection and evaluate the performance of multiplex PCR compared to the serological tests. In the experiment, we optimized the appropriate multiplex PCR for detection of tested bacteria. Subsequently, we evaluated the specificity, sensitivity, and performance of the developed multiplex PCR. Our results showed only positive detectable PCR product from tested bacteria. The sensitivity and specificity of the developed multiplex PCR was 100% and 71.67% compared to the serological tests. And also, the assay found the co-infection of scrub typhus and leptospirosis. The designed multiplex PCR assay is sensitive, specific and rapid. The assay can be used for identifying the causative agent in the early phase of these diseases.
DOI: 10.13145/bacdive137644.20191129.4.1
title: Lactobacillus rhamnosus

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.282
title: Urinary cellular biomarkers as an early diagnostic test for treatment of active lupus nephritis compare with kidney biopsy guided therapy

description: Background: Both patient and renal survival in lupus nephritis depend mainly on treatment response. Early management increase remission rate and improve renal survival. Objective: To demonstrate the better renal remission by the early non-invasive biomarkers testing compared to the conventional approach. Material and method: patients who met proteinuric or nephritic flare criteria were recruited. Biomarker group (N=14) used urine IP-10 to guide treatment. Induction immunosuppression was initiated without waiting for renal pathology. Whereas in the historical cohort (conventional group, N=26) induction therapy was initiated based on renal biopsy results. Primary outcome was overall renal remission. Secondary outcomes were time to remission, adverse event and immunosuppressive dosage Results: The HR of overall renal remission in biomarker group compared to conventional group was 1.23 (95% 0.57-2.67; P=0.595). The mean steroid dosage was 13.1±6.8 vs. 20.7±9.1 P=0.015 and the median time from renal flare to renal remission was 16 weeks (95%CI 9.89-22.1 weeks) vs. 25 weeks (95% CI 13.8-36.2 weeks) in biomarker arm vs. conventional arm respectively. Conclusion: Using urinary IP-10 as LN biomarker couldn’t show better overall renal remission, but can shorten time from renal flare to renal response and decrease net prednisolone dosage. Further larger study is needed to confirm this results.
DOI: 10.13145/bacdive137645.20191129.4.1
title: Lactobacillus paracasei

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive137646.20191129.4.1
title: Haemophilus influenzae

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.283
title: Potentiating effect of cepharanthine in combination with 5-fluorouracil on human colorectal cancer cells

description: 5-Fluorouracil (5-FU) is widely used as a first line drug for patients with colorectal cancer. The use of this drug has however been limited by drug resistance and serious side effects. Several preclinical studies reported that cepharanthine (CEP), a biscoclaurine alkaloid, possesses not only anticancer activity but also chemopotentiation effect. This study intended to determine the potentiating effect of CEP on anticancer effect of 5-FU in colorectal cancer cells. The results clearly demonstrated that CEP significantly enhanced cytotoxic activity of 5-FU in HT-29 cells. Although treatment with 5-FU alone could trigger cancer cells to undergo apoptosis, an increase in necrotic cell death was detected following treatment with 5-FU in combination with CEP. Real-time PCR analysis indicated that down-regulation of Bcl-2 gene expression caused by 5-FU alone was further decreased following treatment with 5-FU and CEP. In addition, the combination treatment could significantly inhibit cell cycle progression in S phase. CEP however did not alter mRNA levels and activities of P-gp, MRP1, or BCRP drug efflux transporters in HT-29 cells. Taken together, the results from this study suggest that the chemopotentiation of 5-FU by CEP may be associated with induction of necrosis, down-regulation of Bcl-2, as well as alteration of cell cycle progression and CEP may potentially be used as an adjuvant agent for enhancing 5-FU potency in colorectal cancer treatment.
DOI: 10.13145/bacdive137647.20191129.4.1
title: Haemophilus influenzae

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.284
title: In vitro study of the effect of gold nanoparticles on SW 620 and HCT 116 colon cancer cell lines

description: Colon cancer acts as a third most leading role in causing death among all the cancer types. Because cancer cells developed resistance against the chemotherapy, radiotherapy, and inability of using surgical treatment methods for advanced metastasis colon cancer, there is a higher demand of more targeted treatment method. Parameter plays a role in cancer metastasis as angiogenesis; vascular endothelial growth factor (VEGF) is as a pro-angiogenic factor for angiogenesis. Colorectal cancer cells have VEGF in very high concentration when compared to normal colon epithelial cells. Based on those findings, scientists developed Bevacizumab, humanized monoclonal antibody, which is capable of binds to all the isomers of VEGF-A. Even though patients treat with targeted therapies, cancer cells show advanced metastasis either by developing new pathways for metastasis or by developing resistance against the treatment. Gold Nanoparticles are a better candidate in various medical applications such as treating cancer as a treatment, drug or gene delivery system, and molecular detection system. Because of its ability to bind with VEGF, gold nanoparticles become the better candidate for cancer treatment. With all these understandings, this study aimed to determine the effect of gold nanoparticles (GNPs) on SW620 and HCT116 colon cancer cell lines. Using SW620 and HCT116 cell lines, treatment with various sizes of Sodium borate (SB) and Sodium citrate (SC) stabilized GNPs for 24h. In our findings, SB stabilized GNPs decreased the cell viability, cell migration and induce cell necrosis. Moreover, SB stabilized GNPs decreased VEGF expression levels in both the cancer cell lines. But, only HCT116 showed decreased HIF-1-alpha expression levels when treated with SB stabilized GNPs. Our results conclude that effect of GNPs on colon cancer cell lines by stabilizing agent dependent pattern. These findings suggest that GNP-SB can be a promising alternative treatment for colorectal malignancy in medicine.
DOI: 10.13145/bacdive137648.20191129.4.1
title: Pseudomonas marginalis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive137649.20191129.4.1
title: Lactobacillus johnsonii

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.285
title: The alternation pattern of estrous cycle modulating electrophysiological properties in primary-cultured trigeminal ganglia neurons

description: Migraine occurs more frequently in women than men. Many women suffer from migraine attacks during menstruation, which are known as menstrual migraine. The aim of this study was to investigate whether different stages of the estrous cycle are involved in migraine development by comparing the excitability and sensitivity of trigeminal ganglion (TG) neurons in four different stages of the estrous cycle by using action potential (AP) parameter assessments performed with whole-cell patch clamp recordings. The result showed that TG neurons in the proestrus and estrus stage had lower AP threshold, lower rheobase, higher AP height, shorter AP falling time and deeper after-hyperpolarization (AHP) depth. In addition, The summation of AP development indicated that TG neurons in proestrus and estrus stages exhibited significantly lower thresholds and rheobase of stimulation, and significant increase of total spikes compared with the TG neurons in the diestrus stage. Our results revealed that fluctuation of estrogen levels in the proestrus and estrus stages altered the single AP properties and summation of AP development of TG neurons. High estrogen levels in proestrus and estrus stages of the estrous cycle may induce increases of neuronal excitability and sensitivity in TG neurons that result in modulation of voltage-gated ion channels and peripheral sensitization. These findings may provide an explanation for the correlation of estrogen fluctuations during the menstrual cycle with the pathogenesis of menstrual migraine.
DOI: 10.13145/bacdive137650.20191129.4.1
title: Lactobacillus gasseri

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.286
title: Using whole exome sequencing to identify mutations of four different human diseases

description: Whole exome sequencing (WES) is an application of the next generation sequencing (NGS). With this technique, the target regions such as coding sequences, splice site, non-coding RNA and highly conserved regions which are about 1 percent of the genome harboring about 85 percent of mutations with large effects on disease-related traits are sequenced. Here, two different Mendelian disorders were studied. The first is familial comedones, a rare autosomal dominant skin disorder. Two unrelated families affected with familial comedones were included. WES combined with whole genome linkage analysis using a single nucleotide polymorphism (SNP) array was conducted in the first family which identified a heterozygous mutation, c.84_85insT in the PSENEN gene. This mutation was also identified in the second family. Quantitative real-time PCR indicated increased expression of PSENEN mRNA in the patients. Another disease included in this study is an undiagnosed syndrome with intellectual disability in a non-consanguineous family. Two siblings were affected. Exome sequencing was performed in both patients and their parents. Neither pathogenic copy number variations nor SNVs/indels were identified. In summary, conducting WES led us to identify a novel gene underlying familial comedones. However, using WES to find a gene underlying a disease with genetic heterogeneity as intellectual disability remains a challenge.
DOI: 10.13145/bacdive137651.20191129.4.1
title: Lactobacillus gasseri

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive137652.20191129.4.1
title: Lactobacillus nantensis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.287
title: Determination of effective doses in image-guided radation therapy system

description: This study determined the imaging doses starting from the planning to the verification process involved in IGRT for clinical protocols of head, chest and pelvis regions. The dose calculation from Varian Acuity simulator and on-board image were performed to obtain the entrance surface air kerma (ESAK). The product of ESAK with the conversion coefficient was resulted as the effective dose. The organ doses from electronic portal imaging device (EPID) were calculated by Varian Eclipse treatment planning system. The tissue weighting factors were applied according to the ICRP 103 for calculating the total effective dose. For the 3D imaging system, computed tomography dose index (CTDI) and cone-beam CT dose index (CBDI) measurements were performed using a 100 mm pencil ionization chamber with Accu-Pro dosimeter and CTDI phantoms. Then, effective doses were calculated by ImPACT software. Moreover, the organ and effective doses from CBCT pelvic protocol was measured by using the thermoluminescent dosimeters inserted in Rando phantom to verify the accuracy of ImPACT calculation. The total effective doses from planning imaging modalities for head, chest and pelvic protocols were 0.03, 0.31 and 0.43 mSv, respectively, from the 2D conventional simulator and 3.3, 13 and 7.2 mSv, from the 3D CT. From the verification imaging modalities, the total effective doses for head, chest and pelvis protocols were 0.018, 0.12 and 0.16 mSv from OBI system and 3.54, 6.24 and 4.86 mSv, respectively from EPID. In kV CBCT, the total effective doses were 0.14 mSv for head, 2.4 mSv for chest and 4.99 mSv for pelvis from the ImPACT calculation and 5.17 mSv for pelvis protocol from TLD measurement. Among the 2D imaging modalities, EPID effective doses were higher than the others because of using the treatment megavoltage beam. In 3D, planning CT doses were higher than those from the CBCT. For CBCT, ImPACT dose calculation result showed 35 % of variation from TLD measurement and it can be applied with acceptable result. Based on 30 fractions of treatment course, total effective doses (3D CT, 2D setup verification and 6 times CBCT) of head, thorax and pelvis were 4.17, 27.71and 37.57mSv, respectively. Therefore, IGRT should be applied carefully to optimize the dose.
DOI: 10.13145/bacdive137653.20191129.4.1
title: Staphylococcus aureus subsp. anaerobius

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.14457/cu.the.2015.288
title: Patient radiation dose from computed tomography angiography and digital subtraction angiography of the brain

description: Computed Tomography Angiography (CTA) and Digital Subtraction Angiography (DSA) are the types of cerebrovascular disorders examination. The 64-row multidetector CTA (64-MDCTA) provides vascular image quality of the brain similar to the DSA, but the effective dose of CTA is 1/5 lower than DSA studied in phantom. The purpose of this study was to evaluate patient effective dose from 64-MDCTA and DSA examination. In this study, the effective dose according to ICRP 103 received by 30 patients (15 male, 15 female, mean age 49 yrs, range 23-89 yrs, mean BMI 24 kg/m2) underwent CTA examination of the brain were determined by the Dose Length Product (DLP) values in the unit of mGy.cm from a 64-row MDCT (Somatom Definition AS; Siemens, Erlangen, Germany) multiplied by the k-factor of 0.0019 mSv/mGy.cm. The effective dose from 30 patients (14 male and 16 female, mean age 47 yrs, range 24-81 yrs, mean BMI 23 kg/m2) from diagnostic cerebral DSA procedure calculated by using Dose Area Product (DAP) values displayed from DAP meter attached in a biplane angiography equipment (Axiom Artis; Siemens, Erlangen, Germany) multiplied by dose conversion coefficient of 0.087 mSv/mGy.cm2. For CTA, the mean effective dose was 3.70 (2.82- 5.19) mSv. The effective dose of CTA depends on patient characteristics (weight, height, and BMI), exposure technique (mAs and kVp) and scan length when fixing other parameters (slice collimation, slice acquisition, pitch, and tube rotation time). In DSA procedure, the mean effective dose was 5.94 (3.30-10.06) mSv. A number of 2D radiography and a number of 3-Dimentional rotational angiography were the key factors affecting the effective dose. The number of vessels selective catheterization and fluoroscopic time were moderate affects the effective dose. The experience of radiologist was no significant correlation with the effective dose. The mean effective dose from DSA procedure was 1.5 time of CTA examination of the brain.
DOI: 10.13145/bacdive137654.20191129.4.1
title: Staphylococcus pasteuri

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive137655.20191129.4.1
title: Salmonella enterica

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.