cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ty2cq-zvt62
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-27/rawdata/</p>PseudoJT2944Tilt6.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-27/rawdata/PseudoJT2944Tilt6.mrc"
        > <i class="download icon"></i> Download </a></p>

        PseudoJT2944Tilt6_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-27/3dimage_94417/PseudoJT2944Tilt6_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.79rbe3
title: Occurrence Download

description: A dataset containing 73 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Galinsoga quadriradiata",
 "Country is Cameroon",
 "HasCoordinate is true"
 ]
}

 The dataset includes 73 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048100-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9uk9af
title: Occurrence Download

description: A dataset containing 721 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Galinsoga quadriradiata",
 "Country is Canada",
 "HasCoordinate is true"
 ]
}

 The dataset includes 721 records from 23 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048101-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/8vagc-3zm19
title: Legionella_delta-lspHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-95/rawdata/</p>DG7_20190516_tomo0095.mrc,  Tilt Series (Pixel Size 0.26 nm), 882.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-95/rawdata/DG7_20190516_tomo0095.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG7_20190516_tomo0095_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-95/3dimage_102813/DG7_20190516_tomo0095_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gdwyg-3c909
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-02</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-02-21/rawdata/</p>Test_Pseudomonas_dfliF_70_fix.mrc,  Tilt Series (Pixel Size 0.37 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-02-21/rawdata/Test_Pseudomonas_dfliF_70_fix.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_70_fix_part101_8.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-02-21/3dimage_97468/Test_Pseudomonas_dfliF_70_fix_part101_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.86rgp3
title: Occurrence Download

description: A dataset containing 565518 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Plant Resources Center"
 ]
}

 The dataset includes 565518 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048087-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/8p3gd-rpn22
title: JV9298

description: DotF-sfGFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2019-10-14</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> PNCC Krios 3</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential/Early stationary</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper. Vitrobot 22 deg C, 100% humidity, 4uL of 2X concentrated culture, blot force 6, blot time 3 sec, drain time 1 sec, wait time 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-10-14-49/rawdata/</p>JV9298_69_D4.mrc,  Tilt Series (Pixel Size 0.1323 nm), 14.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-10-14-49/rawdata/JV9298_69_D4.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_69_D4_full.rec,  Reconstruction (Pixel Size 1.0584 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-10-14-49/3dimage_109509/JV9298_69_D4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.awr6xs
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "PublishingCountry is Colombia",
 {
 "and" : [
 "Country is Colombia",
 {
 "not" : {
 "Issue" : [
 "is Country coordinate mismatch"
 ]
 }
 }
 ]
 }
 ]
 },
 "TaxonKey is one of (Strabomantis anatipes [GBIF Backbone Taxonomy])"
 ]
}

 The dataset includes 13 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048104-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/tww5w-8wz62
title: MEF cells - Septin2 knock down

description: Septin2 was knockdown using retroviral delivery of shRNA. Purpose - mitochondrial division in the absence of Septin2.
description: <strong>Tilt Series Date:</strong> 2019-01-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun-Shik from Chan lab helped design the shRNA against Septin2 and provided transduction protocol. Shrawan and Danielle Grotjahn created cell line. Danielle FIB-milled the cells and Shrawan performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were treated with 10µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-14/rawdata/</p>TS7.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-14/rawdata/TS7.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS7_bin4_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 741.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-14/3dimage_102028/TS7_bin4_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qbngt-88595
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-01</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-49/rawdata/</p>Test_Pseudomonas_dfliF_49_fix.mrc,  Tilt Series (Pixel Size 0.37 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-49/rawdata/Test_Pseudomonas_dfliF_49_fix.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_49_fix_part101_45.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-49/3dimage_97364/Test_Pseudomonas_dfliF_49_fix_part101_45.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.wcqsm8
title: Occurrence Download

description: A dataset containing 20124 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Aphyllon arizonicum, Aphyllon, Aphyllon californicum, Aphyllon corymbosum, Aphyllon fasciculatum, Aphyllon ludovicianum, Aphyllon multiflorum, Aphyllon parishii, Aphyllon pinorum, Aphyllon riparium, Aphyllon robbinsii, Aphyllon uniflorum, Aphyllon validum, Aphyllon vallicolum)",
 "HasCoordinate is TRUE"
 ]
}

 The dataset includes 20124 records from 236 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048103-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bh9cw-5rg70
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-364/rawdata/</p>JV9298_PD-1-3_337.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-364/rawdata/JV9298_PD-1-3_337.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_PD-1-3_337_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-364/3dimage_124375/JV9298_PD-1-3_337_SIRT_1k.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4hsj6-8yh96
title: Legionella_JV9246

description: Legionella_JV9246: DotF-sfGFP fusion to the wild-type gene on the chromosome (thyA) and ΔdotO.
description: <strong>Tilt Series Date:</strong> 2019-05-28</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-42/rawdata/</p>JV9246_area1_tomo0021.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-42/rawdata/JV9246_area1_tomo0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9246_area1_tomo0021_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-42/3dimage_103463/JV9246_area1_tomo0021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xnj6s-k9y77
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-104/rawdata/</p>9082_box1_No1_181.mrc,  Tilt Series (Pixel Size 0.2612 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-104/rawdata/9082_box1_No1_181.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_box1_No1_181_part37_2.rec,  Reconstruction (Pixel Size 1.0448 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-104/3dimage_110259/9082_box1_No1_181_part37_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z1t6e-3bq21
title: ec5-4

description: EC5-4, sq 1, IT 1.2s
description: <strong>Tilt Series Date:</strong> 2018-07-11</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 1094</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 172.5 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Started an ON culture of EColi 1094 in 3mL of liquid LB and induced cellulose production with method #1: Spotted 3uL drops on low salt agar plate and let overnight Scrapped of 1 macrocolony and resuspended in 1mL of liquid low salt LB before freezing</p>
<strong>Sample Preparation:</strong> Manual backside blotting (no glare seen)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-07-11-7/rawdata/</p>sq1_cell4.mrc,  Tilt Series (Pixel Size 0.54 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-07-11-7/rawdata/sq1_cell4.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq1_cell4_full.rec,  Reconstruction (Pixel Size 1.08 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-07-11-7/3dimage_93165/sq1_cell4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.15469673
title: c150_10Gb

description: Synthetic Metagenomic Dataset

Number of samples: 10  - Total size: 88 GB  - Complexity: 60  - Depth: 10 Gb per sample  

Contents:- `processed_read/sample?.?.fastq.gz`: Anonymous processed reads (paired)- `contig/sample?.contigs.fasta`: Gold standard contigs- `gsa_mapping/sample?_gsa_mapping.tsv`: GSA mapping files

Split parts:- Part 1: https://doi.org/10.5281/zenodo.15392822- Part 2: https://doi.org/10.5281/zenodo.15310546- Part 3: https://doi.org/10.5281/zenodo.15380527- Part 4: https://doi.org/10.5281/zenodo.15311049

To reconstruct:cat c150_10Gb_part_* > c150_10Gb.tar.gztar -xzf c150_10Gb.tar.gz
DOI: 10.5281/zenodo.15469674
title: c150_10Gb

description: Synthetic Metagenomic Dataset

Number of samples: 10  - Total size: 88 GB  - Complexity: 60  - Depth: 10 Gb per sample  

Contents:- `processed_read/sample?.?.fastq.gz`: Anonymous processed reads (paired)- `contig/sample?.contigs.fasta`: Gold standard contigs- `gsa_mapping/sample?_gsa_mapping.tsv`: GSA mapping files

Split parts:- Part 1: https://doi.org/10.5281/zenodo.15392822- Part 2: https://doi.org/10.5281/zenodo.15310546- Part 3: https://doi.org/10.5281/zenodo.15380527- Part 4: https://doi.org/10.5281/zenodo.15311049

To reconstruct:cat c150_10Gb_part_* > c150_10Gb.tar.gztar -xzf c150_10Gb.tar.gz