cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/wph17-rgj36
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-90/rawdata/</p>Hpylori_DFliF_FISE_091.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-90/rawdata/Hpylori_DFliF_FISE_091.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_091_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-90/3dimage_113660/Hpylori_DFliF_FISE_091_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3k4yk-gvt63
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-12/rawdata/</p>Hpylori_DFliF_FISE_006.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-12/rawdata/Hpylori_DFliF_FISE_006.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_006_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-12/3dimage_113508/Hpylori_DFliF_FISE_006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1tk3d-p5095
title: pk1-2-3_lam5_ts1

description: .  [added 2019-10-15 12:18:28] IT 0.5s
description: <strong>Tilt Series Date:</strong> 2019-07-20</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK1, BL21 dHisdHA RQABpCDF+EFGpRSF S2-ZC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: °, constant angular increment, dosage: 139.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Petya Krasteva provided the over-expressing lines</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Stock #24 was used to inoculate 3mL of TB + Kana (40ug/mL final) and Strepto (70ug/mL final) at 10am. Cultured 37C - 220rpm. At OD600 1.2 (4:45pm), IPTG (0.7mM final) was added to induce OE of the BCS machinery and cultured ON 15C - 220rpm. At 10:45am (day +1), OD600 was 2.5. Spun down the cells and concentrated at OD600 20.</p>
<strong>Sample Preparation:</strong> Vitrobot set at 27C 95% humidity Manual.3uL of culture backblotting 6s (glare at 4s) DT1'' Grid was FIB milled</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-07-20-7/rawdata/</p>pk1-2-3_lam5_ts1.st,  Tilt Series (Pixel Size 0.45 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-07-20-7/rawdata/pk1-2-3_lam5_ts1.st"
        > <i class="download icon"></i> Download </a></p>

        pk1-2-3_lam5_ts1.rec,  Reconstruction (Pixel Size 1.8 nm), 194.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-07-20-7/3dimage_106859/pk1-2-3_lam5_ts1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x8138-j1836
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-27/rawdata/</p>Hpylori_DFliF_FISE_060.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-27/rawdata/Hpylori_DFliF_FISE_060.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_060_part41_10.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-27/3dimage_113541/Hpylori_DFliF_FISE_060_part41_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/adjfz-raq04
title: Shewanella MR1_Iron cores_Chemos

description: Raw data files of Shewanella MR1_Iron cores_Chemos
description: <strong>Tilt Series Date:</strong> 2018-08-09</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These are Shewanella oneidensis MR1 prepared by Sahand (USC) by Chemostat method grown for 2 days and chemically fixed. I (Mohammed) imaged these cells on the Polara to see if the have the iron cores in order to send them to Israel (Sharon Wolf) to perform EDX and confirm the components of the iron cores.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Chemostat 2 days growth and chemically fixed. The samples were prepared by Sahand (USC).</p>
<strong>Sample Preparation:</strong> I prepared these samples by blot force 6, blot time 3s and drain time 1. I also used the Bdellovibrio conditions (force -5, time 4 s). 10 nm Gold nanoparticles were added.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-56/rawdata/</p>SheweTilt214.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-56/rawdata/SheweTilt214.mrc"
        > <i class="download icon"></i> Download </a></p>

        SheweTilt214_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-56/3dimage_94132/SheweTilt214_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zjw54-d9e16
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-16/rawdata/</p>Bsubtilis_293_008_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-16/rawdata/Bsubtilis_293_008_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_008_af_part41_6.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-16/3dimage_111417/Bsubtilis_293_008_af_part41_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16990945
title: Supplementary S1: Methodology for Calculating Localized Energy Contribution of Spatial-Climatic Design.  Supplementary S2: Derivation of Fenestration System Contribution and Error Propagation.  Supplementary S3: Independent Simulation and Calculation of the Synergy Factor Γ
DOI: 10.5281/zenodo.16990946
title: Supplementary S1: Methodology for Calculating Localized Energy Contribution of Spatial-Climatic Design.  Supplementary S2: Derivation of Fenestration System Contribution and Error Propagation.  Supplementary S3: Independent Simulation and Calculation of the Synergy Factor Γ
DOI: 10.15468/dl.5zkup2
title: Occurrence Download

description: A dataset containing 1651 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Gallus gallus",
 "Country is Indonesia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1651 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006205-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/81430-2mq54
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-104/rawdata/</p>Hpylori_DFliF_FISE_104.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-104/rawdata/Hpylori_DFliF_FISE_104.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_104_part41_4.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-104/3dimage_113692/Hpylori_DFliF_FISE_104_part41_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mtr58-47648
title: pk1-2-3_lam8_ts2

description: .  [added 2019-10-15 12:18:28] IT 0.5s
description: <strong>Tilt Series Date:</strong> 2019-07-20</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK1, BL21 dHisdHA RQABpCDF+EFGpRSF S2-ZC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: °, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Petya Krasteva provided the over-expressing lines</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Stock #24 was used to inoculate 3mL of TB + Kana (40ug/mL final) and Strepto (70ug/mL final) at 10am. Cultured 37C - 220rpm. At OD600 1.2 (4:45pm), IPTG (0.7mM final) was added to induce OE of the BCS machinery and cultured ON 15C - 220rpm. At 10:45am (day +1), OD600 was 2.5. Spun down the cells and concentrated at OD600 20.</p>
<strong>Sample Preparation:</strong> Vitrobot set at 27C 95% humidity Manual.3uL of culture backblotting 6s (glare at 4s) DT1'' Grid was FIB milled</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-07-20-12/rawdata/</p>pk1-2-3_lam8_ts2.st,  Tilt Series (Pixel Size 0.45 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-07-20-12/rawdata/pk1-2-3_lam8_ts2.st"
        > <i class="download icon"></i> Download </a></p>

        pk1-2-3_lam8_ts2.rec,  Reconstruction (Pixel Size 1.8 nm), 328.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-07-20-12/3dimage_106867/pk1-2-3_lam8_ts2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mw5k7-vz208
title: EPEC_8770_U2OS

description: EPEC cells strain 8770 incubated with U2OS. The aim is to see bacterial nanotubes connected  .  to the eukaryotic cell.
description: <strong>Tilt Series Date:</strong> 2020-02-15</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8770, EPEC 8612 + Δbfp ΔtraD-ygeB ΔfinO-traI</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The strain EPEC 8770 is obtained from Ritesh (Ilan Rosenshine lab, the Hebrew university) and . Amit (Sigal ben Yehuda lab). U2OS was obtained from Wei Zhao in the Jensen lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS were grown on gold finders grids overnight in DMEM medium at 37 C. next day the grids were washed with PBS and incubated with DMEM+FCS and bacteria were added . (1:100)  and the grids were cultured for 3.5 hours at 37C. Then the grids were washed twice with PBS and incubated with starvation buffer for 1 hour. The samples were plunge . frozen.</p>
<strong>Sample Preparation:</strong> Samples were blotted manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-15-6/rawdata/</p>EPEC_8770_U2OS_006_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-15-6/rawdata/EPEC_8770_U2OS_006_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8770_U2OS_006_af_part41_1.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-15-6/3dimage_111323/EPEC_8770_U2OS_006_af_part41_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qnfks-hsc21
title: EPEC_8770_U2OS

description: EPEC cells strain 8770 incubated with U2OS. The aim is to see bacterial nanotubes connected  .  to the eukaryotic cell.
description: <strong>Tilt Series Date:</strong> 2020-02-15</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8770, EPEC 8612 + Δbfp ΔtraD-ygeB ΔfinO-traI</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The strain EPEC 8770 is obtained from Ritesh (Ilan Rosenshine lab, the Hebrew university) and . Amit (Sigal ben Yehuda lab). U2OS was obtained from Wei Zhao in the Jensen lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS were grown on gold finders grids overnight in DMEM medium at 37 C. next day the grids were washed with PBS and incubated with DMEM+FCS and bacteria were added . (1:100)  and the grids were cultured for 3.5 hours at 37C. Then the grids were washed twice with PBS and incubated with starvation buffer for 1 hour. The samples were plunge . frozen.</p>
<strong>Sample Preparation:</strong> Samples were blotted manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-15-30/rawdata/</p>EPEC_8770_U2OS_044_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-15-30/rawdata/EPEC_8770_U2OS_044_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8770_U2OS_044_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-15-30/3dimage_111351/EPEC_8770_U2OS_044_af_full.rec"
        > <i class="download icon"></i> Download </a></p>