cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/x3tnn-pe448
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-4/3dimage_6793/</p>010.rec,  Reconstruction (Pixel Size 1.922 nm), 189.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-4/3dimage_6793/010.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/va9wg-39809
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-20/3dimage_6809/</p>032.rec,  Reconstruction (Pixel Size 1.922 nm), 13.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-20/3dimage_6809/032.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/snzn8-t9895
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-19/3dimage_6808/</p>031.rec,  Reconstruction (Pixel Size 1.922 nm), 2.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-19/3dimage_6808/031.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0gm3y-8yc62
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-15/3dimage_6804/</p>022.rec,  Reconstruction (Pixel Size 1.922 nm), 4.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-15/3dimage_6804/022.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dyeh6-hsf26
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-7/3dimage_6796/</p>014.rec,  Reconstruction (Pixel Size 1.922 nm), 64.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-7/3dimage_6796/014.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5284/1134885
title: Digital Archive from an Archaeological Evaluation at St Paul's House, Walton Street, Oxford, Oxfordshire, November 2021

description: This collection consists of site images and records, a report and GIS data from an archaeological evaluation at St Paul's House, Walton Street, Oxford, Oxfordshire. The works were conducted by Oxford Archaeology in November 2021 ahead of proposed residential development. The works comprised monitoring of the removal of concrete foundations and excavation of two trenches. A single pit was uncovered in the south-western part of the site. Finds recovered from the fill date to the 17th-18th centuries.
DOI: 10.22002/s541d-bpd16
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-8/3dimage_6797/</p>015.rec,  Reconstruction (Pixel Size 1.922 nm), 49.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-8/3dimage_6797/015.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cv5xr-ktd93
title: JV1181

description: Legionella thyA ΔdotUF  (JV1181)
description: <strong>Tilt Series Date:</strong> 2017-02-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-22/rawdata/</p>JV1181_02022017a0016.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-22/rawdata/JV1181_02022017a0016.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2q4jy-gez28
title: JV1180_ΔdotUF

description: Legionella thyA ΔdotUF (JV1180). JV1180 is an independent isolate of the UF deletion. JV1180 and JV1181 are same thing.
description: <strong>Tilt Series Date:</strong> 2017-08-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time it was ~2.4.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-16-73/rawdata/</p>20170814_JV1180a0072.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-16-73/rawdata/20170814_JV1180a0072.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ybqc7-k3m74
title: Agro_piliprep7_highmag_16.09.15

description: AD1484:A348dB/pAD2079, virB on a multicopy-plasmidFor pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate.Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to process the data. Grids were little contaminated and data is not very high quality.
description: <strong>Tilt Series Date:</strong> 2015-09-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Patch Tracking</p>
<strong>Collaborators and Roles:</strong> Pili prep from induced strain (AD1484) was done by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> For pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: incubate at 100% humidity for at least 15 min, 4µl sample, blot force 6, blot time 3 sec. Wait time 30 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to process the data.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-16-40/rawdata/</p>AD1484_piliprep7Tile02.mrc,  Tilt Series (Pixel Size 0.32 nm), 786.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-16-40/rawdata/AD1484_piliprep7Tile02.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nhyst-2ww56
title: wt_perfusion_fixed

description: Raw data files of wt_perfusion_fixed
description: <strong>Tilt Series Date:</strong> 2015-09-17</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 145.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> The cells look good on scope. Next time use 1.5ul gold.</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> This sample is wt cells grown in perfusion set-up. It is fixed with 2.5% glutaraldehyde for 15 minutes. The grid was prepared by Sahand on 9/10/15. The grid was triple rinsed in distilled water and saved in distilled water until freezing on 9/15/15 at USC using manual plunger.</p>
<strong>Sample Preparation:</strong> This is gold grid with honey C. The grid was pulled out quickly from media. 1.2ul of 10nm gold was added immediately to the cells on the C side -same side as cells were present. The grid was loaded on manual plunger and blotted from behind- opposite of C side. And then plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-09-17-10/rawdata/</p>091615_perfusionwtfixed_sq50010.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-09-17-10/rawdata/091615_perfusionwtfixed_sq50010.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0p284-f4a10
title: wt_perfusion_fixed

description: Raw data files of wt_perfusion_fixed
description: <strong>Tilt Series Date:</strong> 2015-09-17</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 145.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> The cells look good on scope. Next time use 1.5ul gold.</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> This sample is wt cells grown in perfusion set-up. It is fixed with 2.5% glutaraldehyde for 15 minutes. The grid was prepared by Sahand on 9/10/15. The grid was triple rinsed in distilled water and saved in distilled water until freezing on 9/15/15 at USC using manual plunger.</p>
<strong>Sample Preparation:</strong> This is gold grid with honey C. The grid was pulled out quickly from media. 1.2ul of 10nm gold was added immediately to the cells on the C side -same side as cells were present. The grid was loaded on manual plunger and blotted from behind- opposite of C side. And then plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-09-17-17/rawdata/</p>091615_perfusionwtfixed_sq50017.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-09-17-17/rawdata/091615_perfusionwtfixed_sq50017.mrc"
        > <i class="download icon"></i> Download </a></p>