cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14285963.v1
title: Evaluation of phytochemicals and antioxidant activity of gamma irradiated quinoa (Chenopodium quinoa)

description: Abstract Phytochemical and antioxidant activity of quinoa flour was evaluated after subjected to gamma irradiation processes at dose 3 and 6 kGy. Both non-irradiated and irradiated quinoa samples were subjected to successive extractions in ethanol solvent. The antioxidant activity after gamma irradiation treatment was investigated via Ferric reducing antioxidant power (FRAP) and radical-scavenging activity (RSA) using 2,2-diphenyl-1-picrylhydrazyl (DPPH).Total phenolic and flavonoid content were analyzed using Folin–Ciocalteu micro-method, aluminium chloride (AlCl3) method and High Performance Liquid Chromatography (HPLC). As well as, effect of irradiation treatment on saponin was also evaluated. Irradiation treatment showed slight differences in the saponin content after exposure to 3 and 6 kGy. Irradiation process enhanced both total phenolic content (TPC) and Total flavonoid content (TFC), TPC were 34.52 and 30.92 mg Gallic Acid Equivalent (GAE)/100g compared to 26.25 mg GAE/100g in non-irradiated quinoa. TFC were 67.44 and 62.89 mg Quercetin Equivalents (QE)/100g compared to 53.15 mg QE/100g. Irradiation dose 3 kGy significantly (p&gt; 0.05) decreased the IC50 as DPPH-RSA and increased the FRAP.
DOI: 10.6084/m9.figshare.14285961.v1
title: Proximal composition and fatty acid profile of Hemigrapsus crenulatus (H. Milne Edwards, 1837) as one of the main foods of “patagonian blenny”Eleginops maclovinus (Cuvier, 1830)

description: Abstract The Patagonian blenny (Eleginops maclovinus) is species endemic to South America with physiological characteristics that would facilitate its incorporation into Chilean aquaculture. However, there is currently no specific artificial food that can be used to raise E. maclovinus. In light of this problem, this study describes the proximal composition and fatty acid profile of the crab Hemigrapsus crenulatus, one of the main foods of E. maclovinus. The purpose of the study is to serve as basic information for the development of a specific artificial diet for juveniles of this fish species. The proximal analysis of the complete body of H. crenulatus indicates that it is mainly composed of ash (35.9%), proteins (32.2%), glucides (19.8%) and minor lipids (3.6%). The fatty acid profile is 40.7% PUFAs, 29.7% MUFAs and 29.5% SAFAs, and the most abundant acids are Eicosapentaenoic (18.8%), Oleic (6.8%) and Palmitic (16.6%), respectively. H. crenulatus has highest level of proteins, lipids and PUFAs among the species of the Brachyura infraorder.
DOI: 10.6084/m9.figshare.14285960.v1
title: Evaluation of flour protein for different bread wheat genotypes

description: Abstract Six different bread wheat genotypes; two Egyptian commercial varieties (control); Giza-168 and Gemmeiza-11, and four promising lines; L84 and L148, resulted via hybridization and M10 and M34 via radiation mutation program) were rheologically evaluated using extensograph and for protein, analysis using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The radiation mutant M10 and M34 had the highest maximum resistance which is a very good indicator of strong gluten. The amount of gluten content was higher in M10, L148, and M34 compared to the control samples Gz168 and Gm11. Sulfide amino acids (CYS and MET) are slightly higher in M10. The electrophoretic results and amino acid analyzers show that the best technological quality was exhibited by M10. Radiation mutants wheat genotypes have a protein with good characteristics, mainly gluten which is significantly higher compared to control samples. The rheological properties measured as extensograph and gel electrophoresis were much better in irradiated lines M10 and M34.
DOI: 10.6084/m9.figshare.14285957.v1
title: Phytochemical analysis and biological activities of ethanolic extract of Curcuma longa rhizome

description: Abstract Curcuma longa is an important dietary plant which possess several pharmacological activities, including antioxidant, antimicrobial, anti-inflamatory, anticancer and anti clotting etc. The aim of the present study was to determine the phenolic profile of Curcuma longa and in vitro antioxidant and antidiabetic activities. In HPLC chromatogram of Curcuma longa rhizome extract 15 phenolic compounds were identified namely Digalloyl-hexoside, Caffeic acid hexoside, Curdione, Coumaric, Caffeic acid, Sinapic acid, Qurecetin-3-D-galactoside, Casuarinin, Bisdemethoxycurcumin, Curcuminol, Demethoxycurcumin, and Isorhamnetin, Valoneic acid bilactone, Curcumin, Curcumin-O-glucuronide respectively. The ethanolic extract displayed an IC50 value of 37.1±0.3 µg/ml against alpha glucosidase. The IC50 value of DPPH radical scavenging activity was 27.2 ± 1.1 μg/mL. It is concluded that ethanolic extract of Curcuma long is rich source of curcumin and contain several important phenolics. The in vitro antioxidant and alpha glucosidase inhibitory effect of the plant justifies its popular use in traditional medicine.
DOI: 10.6084/m9.figshare.14285955.v1
title: Co-relationship between Escherichia coli in broiler cellulitis and liver lesions

description: Abstract Pathogenic strains of Escherichia coli may invade the subcutaneous tissue of poultry and cause cellulitis, whilst the pathogen may also cause lesions in internal organs such as the liver. Current paper co-relates Escherichia coli and virulence genes characteristic of Avian Pathogenic Escherichia coli (APEC) in broilers´ cellulitis and liver lesions. One hundred carcasses were retrieved from the production chain in an avian abattoir in the state of Bahia, Brazil, between August 2013 and January 2014, due to detection of cellulitis lesions. Cellulitis and liver samples were retrieved aseptically to quantify E. coli by Petrifilm™ count fast method (3M Company) (AOAC 998.8). Virulent genes iss and iutA were removed from E. coli isolates by Polymerase Chain Reaction (PCR). Escherichia coli was isolated from 82.0% of broilers removed from the production chain and the bacterium was concomitantly detected in cellulitis and liver lesions in 40.0% of broilers. E. coli counts ranged between 1.00 and 4.73 log CFU/g in liver lesions and between 2.00 and 9.00 log UFC/g in cellulitis lesions. Virulent genes iutA and iss were detected in 97.56% and 89.02% of E. coli isolates, respectively. Genotype analysis demonstrated the concomitant amplification of genes iutA and iss in 60.0% (n=40) of samples of cellulitis and liver lesions in which the simultaneous isolation of E. coli occurred. There was a positive and significant co-relationship (r=0.22; p&lt;0.05) between the variables occurrence of E. coli isolated from liver samples and the occurrence of E. coli isolated from cellulitis lesions. There were also positive and significant co-relationships between populations of E. coli from liver isolates and cellulitis lesions (r=0.46; p&lt;0.05) when E. coli isolated in the liver and in cellulitis lesions was detected. Since results showed a relationship between E. coli in cellulitis and liver lesions and possible systemic infection, the occurrence of cellulitis lesions as a criterion for total discarding of carcass may be suggested.
DOI: 10.6084/m9.figshare.14285952.v1
title: Chemical composition and biological activities of essential oil from flowers of Psidium guajava (Myrtaceae)

description: Abstract Xylella fastidiosa is a plant-pathogenic bacterium that lives inside host xylem vessels, where it forms biofilm which is believed to be responsible for disrupting the passage of water and nutrients. Pectobacterium carotovorum is a Gram-negative plant-specific bacterium that causes not only soft rot in various plant hosts, but also blackleg in potato by plant cell wall degradation. Chagas disease, which is caused by Trypanosoma cruzi, has been commonly treated with nifurtimox and benzonidazole, two drugs that cause several side effects. As a result, the use of natural products for treating bacterial and neglected diseases has increased in recent years and plants have become a promising alternative to developing new medicines. Therefore, this study aimed to determine, for the first time, the chemical composition of essential oil from Psidium guajava flowers (PG-EO) and to evaluate its in vitro anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi and cytotoxic activities. PG-EO was obtained by hydrodistillation in a Clevenger apparatus while its chemical composition was determined by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Major compounds identified in PG-EO were α-cadinol (37.8%), β-caryophyllene (12.2%), nerolidol (9.1%), α-selinene (8.8%), β-selinene (7.4%) and caryophyllene oxide (7.2%). Results showed that the PG-EO had strong trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi (IC50 = 14.6 μg/mL), promising antibacterial activity against X. fastidiosa (MIC = 12.5 μg/mL) and P. carotovorum (MIC = 62.5 μg/mL), and moderate cytotoxicity against LLCMK2 adherent epithelial cells in the concentration range (CC50 = 250.5 μg/mL). In short, the PG-EO can be considered a new source of bioactive compounds for the development of pesticides and trypanocide drugs.
DOI: 10.6084/m9.figshare.14285949.v1
title: Actin filaments altered distribution in wheat (Triticum aestivum) “Bending Root” to respond to enhanced Ultraviolet-B radiation

description: Abstract Plants adjust their shoot growth to acclimate to changing environmental factors, such as to enhanced Ultraviolet-B (UV-B) radiation. However, people have ignored that plant roots can also respond to UV-B light. Here, we find the morphology curled wheat roots under UV-B radiation, that we call, “bending roots.” The curly region is the transition zone of the root after observed at the cellular level. After exposed to enhanced UV-B radiation for 2 d (10.08 KJ/m2/d), cell size decreased and actin filaments gathered in wheat roots. We also find that H2O2 production increased and that content of the indole-3-acetic acid (IAA) increased remarkably. The pharmacological experiment revealed that actin filaments gathered and polymerized into bundles in the wheat root cells after irrigated H2O2 and IAA. These results indicated that actin filaments changed their distribution and formed the “bending root,” which was related to H2O2 production and increase in IAA. Overall, actin filaments in wheat root cells could be a subcellular target of UV-B radiation, and its disruption determines root morphology.
DOI: 10.6084/m9.figshare.14285948.v1
title: Zooplankton communities and their relationship with water quality in eight reservoirs from the midwestern and southeastern regions of Brazil

description: Abstract Zooplankton are widely recognised as being regulated primarily by predators and food availability. In reservoirs, the quantity and quality of food resources are generally affected by the characteristics of the water, which in turn are controlled by the flow pulse generated by operation of the dams. In this study, we investigated the relationship between zooplankton, water quality and food availability (phytoplankton) in eight hydroelectric reservoirs located in Brazil. Samples were collected during the rainy and dry periods between 2008 and 2009. In general, the reservoirs exhibited mesotrophic conditions and Cyanobacteria were the predominant phytoplankton. The results showed that the rotifers Kellicottia bostoniensis, Hexarthra mira, Keratella spp., and Polyarthra vulgaris were present, indicating nutrient-rich environments. In addition, the copepod Thermocyclops decipiens occurred in eutrophic environments. In contrast, the cladoceran Daphnia gessneri and copepod Notodiaptomus henseni were considered indicators of more desirable water quality, owing to their relationship with waters with lower levels of nutrients and suspended solids. The results support the use of these organisms as a useful tool for understanding changes in water quality and in the ecosystem processes involved.
DOI: 10.6084/m9.figshare.14285947.v1
title: Seed-dispersing bird assemblages in riparian environments of a partially urbanized river basin, Southeastern Brazil

description: Abstract Birds play a key role in ecosystem dynamics, including urban and rural areas, bringing environmental quality improvements and ecological stability. Species contribute directly to natural regeneration of vegetation and succession processes, by offering ecosystem services as seed dispersal, an important role in human-modified areas. We studied the assemblages of fruit-eating birds in riparian environments of Monjolinho basin, central São Paulo state, southeastern Brazil. Birds were recorded in 41 points distributed in riparian ecosystems alongside waterbodies, in landscapes with five types of surrounding matrices: urban, periurban, farmland, and native vegetation. We described how assemblages are structured aiming to evaluate the possible influence of seasonality and landscape type. We recorded 39 bird species that can play a role as seed-dispersers, 32 in wet season and 32 in dry season. There were no significant differences in the diversity and dominance of species between seasons considering the entire area, indicating stability of basic assemblage structure. However, total number of individuals of all species recorded in different landscapes were influenced by seasonality. Also, the composition and abundance of species significantly changed between seasons, leading to a high dissimilarity with almost 50% of the species contributing with almost 90% of the observed variation. A higher taxonomic diversity and distinctness pointed to a wider array of possible seed dispersal services in natural areas, while the lowest values of indexes were found in human-modified areas. The higher number of non-related bird species during dry season contrasted with the higher number of individuals during wet season, indicating that there is more possible ecosystem services offered by frugivorous birds in driest period of the year, while in the rainy period the carrying capacity of the riparian environments was increased.
DOI: 10.5281/zenodo.4279220
title: Test dataset for separation of speech, traffic sounds, wind noise, and general sounds

description: The dataset was generated as part of the paper:<br> Deep Complex U-Net Ensemble for Outdoor Urban Sound Source Separation,<br> K. Arendt, A. Szumaczuk, B. Jasik, P. Masztalski, K. Piaskowski, M. Matuszewski, K. Nowicki, P. Zborowski. It contains various sounds from the Audio Set [1] and spoken utterances from VCTK [2] and DNS [3] datasets. Contents:<br> sr_8k/<br> mix_clean/<br> s1/<br> s2/<br> s3/<br> s4/<br> sr_16k/<br> mix_clean/<br> s1/<br> s2/<br> s3/<br> s4/<br> sr_48k/<br> mix_clean/<br> s1/<br> s2/<br> s3/<br> s4/ Each directory contains 512 audio samples in different sampling rate (sr_8k - 8 kHz, sr_16k - 16 kHz, sr_48k - 48 kHz).<br> The audio samples for each sampling rate are different as they were generated randomly and separately.<br> Each directory contains 5 subdirectories:<br> - mix_clean - mixed sources,<br> - s1 - source #1 (general sounds),<br> - s2 - source #2 (speech),<br> - s3 - source #3 (traffic sounds),<br> - s4 - source #4 (wind noise). The sound mixtures were generated by adding s2, s3, s4 to s1 with SNR ranging from -10 to 10 dB w.r.t. s1. <br> REFERENCES: [1] Jort F. Gemmeke, Daniel P. W. Ellis, Dylan Freedman,<br> Aren Jansen, Wade Lawrence, R. Channing Moore,<br> Manoj Plakal, and Marvin Ritter, “Audio set: An ontology<br> and human-labeled dataset for audio events,” in<br> Proc. IEEE ICASSP 2017, New Orleans, LA, 2017. [2] Christophe Veaux, Junichi Yamagishi, and Kirsten Mac-<br> Donald, “CSTR VCTK corpus: English multi-speaker<br> corpus for CSTR voice cloning toolkit, [sound],”<br> https://doi.org/10.7488/ds/1994, University of Edinburgh.<br> The Centre for Speech Technology Research<br> (CSTR). 2017. [3] Chandan K. A. Reddy, Ebrahim Beyrami, Harishchandra<br> Dubey, Vishak Gopal, Roger Cheng, Ross Cutler,<br> Sergiy Matusevych, Robert Aichner, Ashkan Aazami,<br> Sebastian Braun, Puneet Rana, Sriram Srinivasan, and<br> Johannes Gehrke, “The interspeech 2020 deep noise<br> suppression challenge: Datasets, subjective speech<br> quality and testing framework,” 2020.
DOI: 10.6084/m9.figshare.14285946.v1
title: Optimized broad-range real-time PCR-based method for bacterial screening of platelet concentrates

description: Abstract Bacterial contamination of blood components remains a major challenge in transfusion medicine, particularly, platelet concentrates (PCs) due to the storage conditions that support bacterial proliferation. In this study, we develop a rapid, sensitive and specific real-time PCR protocol for bacterial screening of PCs. An internally controlled real-time PCR-based method was optimized and validated with our proprietary 16S Universal PCR Master Mix (IBMP/Fiocruz), which targets a conserved region of the bacterial 16S rRNA gene. Nonspecific background DNA was completely eliminated by treating the PCR Master Mix with ethidium monoazide (EMA). A lower limit of detection was observed for 10 genome equivalents with an observed Ct value of 34±1.07 in calibration curve generated with 10-fold serial dilutions of E. coli DNA. The turnaround time for processing, including microbial DNA purification, was approximately 4 hours. The developed method showed a high sensitivity with no non-specific amplification and a lower time-to-detection than traditional microbiological methods, demonstrating it to be an efficient means of screening pre-transfusion PCs.
DOI: 10.5281/zenodo.4279219
title: Test dataset for separation of speech, traffic sounds, wind noise, and general sounds

description: The dataset was generated as part of the paper:<br> Deep Complex U-Net Ensemble for Outdoor Urban Sound Source Separation,<br> K. Arendt, A. Szumaczuk, B. Jasik, P. Masztalski, K. Piaskowski, M. Matuszewski, K. Nowicki, P. Zborowski. It contains various sounds from the Audio Set [1] and spoken utterances from VCTK [2] and DNS [3] datasets. Contents:<br> sr_8k/<br> mix_clean/<br> s1/<br> s2/<br> s3/<br> s4/<br> sr_16k/<br> mix_clean/<br> s1/<br> s2/<br> s3/<br> s4/<br> sr_48k/<br> mix_clean/<br> s1/<br> s2/<br> s3/<br> s4/ Each directory contains 512 audio samples in different sampling rate (sr_8k - 8 kHz, sr_16k - 16 kHz, sr_48k - 48 kHz).<br> The audio samples for each sampling rate are different as they were generated randomly and separately.<br> Each directory contains 5 subdirectories:<br> - mix_clean - mixed sources,<br> - s1 - source #1 (general sounds),<br> - s2 - source #2 (speech),<br> - s3 - source #3 (traffic sounds),<br> - s4 - source #4 (wind noise). The sound mixtures were generated by adding s2, s3, s4 to s1 with SNR ranging from -10 to 10 dB w.r.t. s1. <br> REFERENCES: [1] Jort F. Gemmeke, Daniel P. W. Ellis, Dylan Freedman,<br> Aren Jansen, Wade Lawrence, R. Channing Moore,<br> Manoj Plakal, and Marvin Ritter, “Audio set: An ontology<br> and human-labeled dataset for audio events,” in<br> Proc. IEEE ICASSP 2017, New Orleans, LA, 2017. [2] Christophe Veaux, Junichi Yamagishi, and Kirsten Mac-<br> Donald, “CSTR VCTK corpus: English multi-speaker<br> corpus for CSTR voice cloning toolkit, [sound],”<br> https://doi.org/10.7488/ds/1994, University of Edinburgh.<br> The Centre for Speech Technology Research<br> (CSTR). 2017. [3] Chandan K. A. Reddy, Ebrahim Beyrami, Harishchandra<br> Dubey, Vishak Gopal, Roger Cheng, Ross Cutler,<br> Sergiy Matusevych, Robert Aichner, Ashkan Aazami,<br> Sebastian Braun, Puneet Rana, Sriram Srinivasan, and<br> Johannes Gehrke, “The interspeech 2020 deep noise<br> suppression challenge: Datasets, subjective speech<br> quality and testing framework,” 2020.
DOI: 10.6084/m9.figshare.14285943.v1
title: Validation of a documentary on Chagas disease by a population living in an endemic area

description: Abstract Educational interventions may trigger actions that contribute to prevent parasitic diseases, such as Chagas disease (CD). This study aimed at investigating the impact of an instructional video named “Documentary on Chagas Disease” on knowledge about CD and its vectors displayed by a population that lives in an endemic area in Brazil, so as to validate it as an educational tool. The video was shown to 226 subjects, divided into two groups. Group 1 was composed of users of Basic Health Units (BHU) in Pelotas and Pinheiro Machado, cities located in Rio Grande do Sul (RS) state, Brazil, where CD is endemic. Group 2 consisted of students who attend three public schools located in the rural area in Pinheiro Machado, RS. Two questionnaires with questions about their knowledge about triatomines and CD were applied, before and after the documentary was shown. After the video was shown, there was significant increase in individuals’ knowledge (in both groups) about “kissing bugs”, their notification, the disease and its prevention. Besides, watchers considered that the quality of the material was satisfactory. Since the “Documentary on CD” can be easily accessed on the internet and was effective in teaching the population that lives in endemic areas, its use should be encouraged in places and meetings connected to health that aim at fighting against triatominae and at exposing an updated view of CD.
DOI: 10.6084/m9.figshare.14285942.v1
title: Evaluation of eight protocols for genomic DNA extraction of Hypostomus commersoni Valenciennes, 1836 (Loricariidae: Siluriformes)

description: Abstract The principle and the techniques applied in DNA extraction play a pivotal role in the obtention of a purified genetic material. The present study investigates the efficiency of eight protocols in the DNA extraction of Hypostomus commersoni, an essential component of South American freshwater ichthyofauna. The quality of samples was assessed through spectrophotometry, gel electrophoresis, and PCR-RAPD markers amplification. The efficiency of DNA extraction was influenced both by the method applied and the target-tissue of choice. Higher concentrations and yield of DNA were obtained from ocular tissue, with a positive spectrum of incubation in lysis buffer for up to 36 hours after sample collection, using fresh tissues and in the presence of a high concentration of Proteinase K (20 mg.ml-1). In these conditions, samples were successfully amplified. To date, there is no record of description for the parameters analyzed in this work, neither the description of RAPD markers for the species H. commersoni.