DOI: 10.5061/dryad.r841282
title: Data from: Teratological and behavioral screening of the National Toxicology Program 91-compound library in zebrafish (Danio rerio)

description: To screen the tens of thousands of chemicals for which no toxicity data
 currently exists, it is necessary to move from in vivo rodent models to
 alternative models, such as zebrafish. Here, we used dechorionated
 Tropical 5D wildtype zebrafish embryos to screen a 91-compound library
 provided by the National Toxicology Program (NTP) for developmental
 toxicity. This library contained 86 unique chemicals that included
 negative controls, flame retardants, polycyclic aromatic hydrocarbons
 (PAHs), drugs, industrial chemicals and pesticides. Fish were exposed to
 five concentrations of each chemical or an equal amount of vehicle (0.5%
 DMSO) in embryo medium from 6 h post-fertilization (hpf) to 5 d
 post-fertilization (dpf). Fish were examined daily for mortality and
 teratogenic effects and photomotor behavior was assessed at 4 and 5 dpf.
 Of the five negative control compounds in the library, none caused
 mortality/teratogenesis, but two altered behavior. Chemicals provided in
 duplicate produced similar outcomes. Overall, 13 compounds caused
 mortality/teratology but not behavioral abnormalities, 24 only affected
 behavior, and 18 altered both endpoints, with behavior affected at
 concentrations that did not cause mortality/teratology (55/86 hits). Of
 the compounds that affected behavior, 52% caused behavioral abnormalities
 at either 4 or 5 dpf. Compounds within the same functional group caused
 different behavioral abnormalities, while similar behavioral patterns were
 caused by compounds from different groups. Our data suggest that behavior
 is a sensitive endpoint for developmental toxicity screening that
 integrates multiple modes of toxic action and is influenced by the age of
 the larval fish at the time of testing.
description: Behavior
 ScreenZip file containing
 Excel files of photomotor behavior data assessed at 4 and 5 days
 post-fertilization for each individual chemical. The sheets titled “4 dpf”
 and “5 dpf” show the total distance moved in mm per 1 min bins and per
 light cycle for individual larvae. Sheets “4 dpf Behavior,” “4 dpf AUC,”
 “5 dpf Behavior” and “5 dpf AUC” are graphical depictions of this data.
 Sheet “Sheet1” is the statistical summary of the analysis and sheets
 “Sheet2” through “Sheet 13” are the cycle-specific results of Equation 1
 (see section titled "Behavior screen" in
 paper).Behavior.zipMortality/teratology screenAt 1, 2, 3, 4, and 5 dpf, embryos were examined for mortality and developmental malformations. Traditional endpoints of zebrafish teratology were scored, including arrested development, yolk sac edema, pericardial edema, red heart, deformities of the body axis, notochord aberrations, and malformations of craniofacial structures or the caudal fin (Fig. 2). Morphological assessments were quantified using a binary scale of either normal or abnormal morphology. For each exposure and each endpoint in the screen, the number of fish that were dead or exhibited a teratogenic effect was divided by the total number of fish within each group (n = 32 per exposure). If the incidence of mortality or of any individual teratogenic endpoint within any exposure group was more than 30% greater than that observed for the DMSO controls within the same spawn, it was considered a hit.Morphology_Dryad.xlsx