DOI: 10.7272/q6vx0dsd
title: Multiplex IgG antibody response and malaria parasitemia among children ages 1-59 months in the MORDOR Niger trial, 2015-2018

description: We measured IgG responses to several malaria, bacterial, and protozoan
 pathogens using a multiplex bead assay in pre-specified substudy of 30
 communities in the rural, MORDOR Niger placebo-controlled trial over a
 three-year period (n=5,642 blood specimens, n=3,814 children ages 1-59
 months). We compared seroprevalence and serological force of
 infection between 15 communities that received biannual mass distribution
 of azithromycin versus placebo. The study included antigens to: Plasmodium
 falciparum (MSP-1, AMA1, GLURP-Ro, LSA1, CSP, HRP2), P. vivax (MSP-1), P.
 ovale (MSP-1), P. malariae (MSP-1), Campylobacter jejuni (p18, p39),
 enterotoxigenic Echerichia coli ( ETEC LTB), Vibrio cholerae (CTB),
 Salmonella enterica (LPS serogroups B, D), Cryptosporidium parvum (Cp17,
 Cp23), Giardia duodenalis (VSP-3, VSP-5), and Streptococcus group A
 (SPEB).
description: Communities with 200 to 2,000 inhabitants based on the Niger 2012
 census were eligible for inclusion in the trial, and children ages 1–59
 months who weighed &gt;3.8 kg were eligible for treatment. An
 intensive morbidity monitoring trial enrolled 30 communities and
 randomized them 1:1 to receive either biannual azithromycin or placebo to
 all children 1-59 months old (NCT02048007). The trial used a repeated
 cross-sectional design, whereby 40 children per community were randomly
 sampled in each measurement round and invited to participate in a
 monitoring visit. The trial’s open cohort design meant that children aged
 in and out of the study based on their age at the time of treatment. Field
 staff collected dried blood spots from participating children at baseline
 and annually thereafter at 12, 24, and 36 months of follow-up. The
 antibody substudy included a supplemental visit at 6 months, following the
 malaria season. Children who were randomly selected in multiple survey
 rounds contributed to longitudinal analyses. Dried
 blood spots were tested using a multiplex bead assay on the Luminex
 platform for IgG responses. Separate blood specimens were assessed by
 microscopy for malaria parasitemia. The associated manuscript includes
 specimen testing details. The study included antigens to:
 <em>Plasmodium falciparum</em> (MSP-1, AMA1, GLURP-Ro, LSA1,
 CSP, HRP2), <em>P. vivax</em> (MSP-1), <em>P.
 ovale</em> (MSP-1), <em>P. malariae</em> (MSP-1),
 <em>Campylobacter jejuni</em> (p18, p39), enterotoxigenic
 <em>Escherichia coli</em> ( ETEC LTB), <em>Vibrio
 cholera</em>e (CTB), <em>Salmonella enterica</em> (LPS
 serogroups B, D), <em>Cryptosporidium parvum </em>(Cp17,
 Cp23), <em>Giardia </em><i>duodenalis</i> (VSP-3,
 VSP-5), and <em>Streptococcus </em>group A (SPEB). In addition
 to pathogens included in the analysis, the dataset additionally includes
 antibody responses to <em>Chylamydia trachomatis </em>(Pgp3,
 CT694) and <em>Strongyloides stercoralis </em>(NIE), which
 showed no evidence of seropositivity in this setting but have been
 included for completeness.
description: This repository includes data that underlies the analyses in the
 manuscript entitled <em>Biannual azithromycin distribution and
 antibody responses to malaria, bacterial, and protozoan pathogens among
 Nigerien children</em> There are two
 datasets: mordor-ab-analysis-public.csv/.rds includes
 all antibody data in long format (unique observation is
 child-visit-antigen)
 mordor-ab-treatment-public.csv/.rds includes treatment status for
 all children 1-59 months in the 30 communities. There were more children
 (n=9,066) who were measured for treatment status than the random sample of
 up to 40 children per community who were enrolled in the antibody substudy
 in each visit. Full replication materials are
 cross-linked in the Open Science Framework (https://osf.io/954bt/)
 and GitHub (https://github.com/proctor-ucsf/mordor-antibody). To replicate the study's analysis, we recommend these steps. (1) Clone the GitHub repository to your local drive (mordor-antibody); (2) download the datasets from Dryad (here) or OSF; (3) create a mordor-antibody/<strong>data</strong> subdirectory and copy the two datasets (.csv / .rds) into it; (4) finally, create a mordor-antibody/<strong>figures</strong> subdirectory to store output. All of the analysis scripts should run smoothly. The first two data processing scripts will not run because they read in our internal datasets with PII. We have included them for transparency and completeness. Should you have any questions about the files in this repository, please contact Ben Arnold at UCSF (ben.arnold@ucsf.edu).