DOI: 10.5061/dryad.96m2c
title: Data from: Strong premating reproductive isolation drives incipient speciation in Mimulus aurantiacus

description: Determining which forms of reproductive isolation have the biggest impact
 on the process of divergence is a major goal of speciation research. These
 barriers are often divided into those that affect the potential for
 hybridization (premating isolation), and those that occur after mating
 (postmating isolation), and much debate has surrounded the relative
 importance of these categories. Within the species Mimulus aurantiacus,
 red- and yellow-flowered ecotypes occur in the southwest corner of
 California, and a hybrid zone occurs where their ranges overlap. We show
 that premating barriers are exclusively responsible for isolation in this
 system, with both ecogeographic and pollinator isolation contributing
 significantly to total isolation. Postmating forms of reproductive
 isolation have little or no impact on gene flow, indicating that hybrids
 likely contribute to introgression at neutral loci. Analysis of molecular
 variation across thousands of RAD-seq markers reveals that the genomes of
 these taxa are largely undifferentiated. However, structure analysis shows
 that these taxa are distinguishable genetically, likely due to the impact
 of loci underlying differentiated adaptive phenotypes. These data exhibit
 the power of divergent natural selection to maintain highly differentiated
 phenotypes in the face of gene flow during the early stages of speciation.
description: env_layersenv_layers.zip this file contains the environmental data used in species distribution modeling in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution Within the folder, there are 10 .asc files that contain the gridded environmental data specific to San Diego County, CA, USA. Files come from the following sources: 1) bias.asc: a bias layer incorporated into distribution modeling to account for variation in grid size of Worldclim layers across relatively large spans of latitude. 2) bio1.asc: Annual Mean Temperature 3) bio4.asc: Temperature Seasonality 4) bio5.asc: Max Temperature of Warmest Month 5) bio6.asc: Min Temperature of Coldest Month 6) bio12.asc: Annual Precipitation 7) bio15.asc: Precipitation Seasonality 8) bio16.asc: Precipitation of Wettest Quarter 9) bio17.asc: Precipitation of Driest Quarter 10) geology.asc: basic geologic bedrock material climate data are from: 8 Bioclim layers from the publicly available Worldclim current conditions (~1950-2000) 30 arc-second resolution Global Climate Data database address: http://www.worldclim.org/bioclim accessed: February 1, 2012 Geology data were from: US Geologic Survey address: www.usgs.gov accessed: February 1, 2012 Geology data are coded as numerical categories, with the following key: VALUE ROCKTYPE 0 andesite 1 argillite 2 greenschist 3 sandstone 4 diorite 5 peridotite 6 intermediate volcanic rock 7 gabbro 8 alluvium 9 water 10 slate 11 schist 12 mafic volcanic rock 13 serpentinite 14 landslide 15 dune sand 16 blueschist 17 glacial drift 18 conglomerate 19 phyllite 20 limestone 21 mudstone 22 shale 23 dacite 24 granodiorite 25 gneiss 26 sand 27 greenstone 28 plutonic rock 29 metasedimentary rock 30 chert 31 melange 32 quartzite 33 basalt 34 rhyolite 35 siltstone 36 hornfels 37 metavolcanic rock 38 felsic volcanic rock 39 tephrite (basanite) 40 dolostone 41 trachybasalt 42 peraluminous granite 43 mica schist 44 quartz monzonite 45 orthoquartzite 46 granite 47 marble 48 anorthosite 49 tonalite 50 quartz diorite 51 pelitic schistvariable_extractionvariable_extraction_readme this file contains environmental data for locations of Mimulus aurantiacus populations in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution each row represents an individual population record. the columns represents 1) an abbreviated name given to each population, 2) the ecotype of the population, 3) longitude of the population, 4) latitude of the population, 5-13) values for each environmental variable. see env_var_buffers_readme for description of variables.pollinator_visitationpollinator_visitation_readme This file contains data used to calculate pollinator reproductive isolation in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution each row represents a different bout by a specific pollinator the columns represent: 1) a name for the array site, 2) the day observations were performed, 3) the kind of pollinator [HB=hummingbird; HM=hawkmoth], 4) the number of transitions from one red-flowered plant to another, 5) the number of transitions from one red-flowered plant to a yellow-flowered plant, 6) the number of transitions from one yellow-flowered plant to a red-flowered plant, 7) the number of transitions from one yellow-flowered plant to anotherpollen_comppollen_comp_readme This file contains data used to calculate gametic reproductive isolation in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution each row represents a specific pollen competition cross the columns represent: 1) an identification number for the cross, 2) the flower color of the maternal plant, 3) the name of the maternal population, 4) the name of the paternal population from the alternate ecotype, 5) the count of homozygous offsrping, 6) the count of heterzygous offspring, 7) the frequency of heterozygous offspringwered plant to anotherseed_setseed_set_readme This file contains data used to calculate seed set isolation in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution each row represents a specific cross the columns represent: 1) an identification number for the cross, 2) the category of cross, 3) the population of the maternal parent, 4) the ecotype of the maternal parent, 5) the family of the maternal parent, 6) the individual of the maternal parent within the family, 7) the population of the paternal parent, 8) the ecotype of the paternal parent, 9) the family of the paternal parent, 10) the individual of the paternal parent within the family, 11) the number of seeds producedhybrid_viabilityhybrid_viability_readme This file contains data used to calculate intrinsic postzygotic isolation due to relative hybrid viability in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution each row represents a specific cross, and 32 offspring were assayed per cross the columns represent: 1) the combination of populations involved in the cross (see key below), 2) the category of the cross, 3) the number of individuals that germinated out of 32, 4) the frequency of germination, 5) the number of dead individuals out of 32, 6) the frequency of mortality, 7) the frequency that survived key to population_combo 1 = red ecotype; ucsd population 2 = red ecotype; elf population 3 = red ecotype; lh population 4 = yellow exotype; lo population 5 = yellow ecotype; pct population 6 = yellow ecotype; potr populationpollen_viabilitypollen_viability_readme This file contains data used to calculate intrinsic postzygotic isolation due to relative hybrid fertility in "STRONG PREMATING REPRODUCTIVE ISOLATION DRIVES INCIPIENT SPECIATION IN MIMULUS AURANTIACUS" Sobel and Streisfeld, Evolution each row represents one replicate the columns represent: 1) the category of the cross, 2) the combination of populations involved in the cross (see key below), 3) the cross level replicate number, 4) the specific offspring of each cross replicate, 5) the sample replicate number within a plant, 6) the count of inviable pollen grains out of 200 total counted, 7) the frequency of inviable pollen grains key to population_combo 1 = red ecotype; ucsd population 2 = red ecotype; elf population 3 = red ecotype; lh population 4 = yellow exotype; lo population 5 = yellow ecotype; pct population 6 = yellow ecotype; potr populationSRA212479Raw reads from a 100bp single-end Illumina 2000 sequencing run of a Pst-I generated RAD-seq library. Barcodes are sorted into individual fastq files for each member of the 6 populations sequenced. Filenames begin with the population name, followed by a unique individual number. Red-ecotype populations sequenced are: ucsd, elf, and lh; yellow-ecotype populations are: lo, pct, and potr.