DOI: 10.5061/dryad.qfttdz0qs
title: Data from: Interacting proteome of PtDAL10 and PtDAL1 in Pinus tabuliformis

description: The optimal timing of the transition from vegetative growth to
 reproductive growth is critical for plant reproductive success, and the
 underlying regulatory mechanisms have been well studied in angiosperm
 model species, but relatively little in gymnosperms. Recently, DAL1, a
 MADS domain transcription factor (TF) gene that shows a conserved
 age-related expression profile in conifers, was found to serve as an age
 timer. However, how the DAL1 mediates the onset of reproductive growth
 remains poorly understood. Here, we have shown that the PtDAL1 directly
 regulates the PtDAL10 transcription by binding to its promoter region,
 resulting in a ternary MADS-domain TF complex with PtDAL10 and PtMADS11,
 two potential candidate regulators of the vegetative to reproductive
 transition in Chinese pine (Pinus tabuliformis). The PtDAL10 was
 progressively induced in new shoots with age and highly accumulated in
 male and female cones. Overexpression of PtDAL10 rescued the flowering of
 ft-10 and soc1-1-2 mutants in Arabidopsis. We provide insight into the
 molecular components associated with the PtDAL1, which integrates the
 vegetative to reproductive phase transition into age-mediated progressive
 development of the whole plant in conifers.
description: The Y2H-seq screening method was established and described as
 previously (Chen et al., 2021). The BD-PtDAL10 construct was used as bait
 and the yeast library as prey to screen for the interacting proteins. Each
 monoclonal colony was considered as an event. One thousand events were
 selected and sequenced by next generation sequencing (NGS). Sequencing was
 performed on the Illumina NovaSeq 6000 platform, which was provided by
 Beijing Genewiz Biotechnology Co (https://www.genewiz.com.cn/). The final
 non-redundant proteins interacting with PtDAL10 were identified.
description: # Data from: Interacting proteome of PtDAL10 and PtDAL1 in Pinus
 tabuliformis
 [https://doi.org/10.5061/dryad.qfttdz0qs](https://doi.org/10.5061/dryad.qfttdz0qs) ## Description of the data and file structure The Y2H-seq screening method was established and described as previously (Chen et al., 2021). The BD-PtDAL10 construct was used as bait and the yeast library as prey to screen for the interacting proteins. Each monoclonal colony was considered as an event. One thousand events were selected and sequenced by next generation sequencing (NGS). Sequencing was performed on the Illumina NovaSeq 6000 platform, which was provided by Beijing Genewiz Biotechnology Co ([https://www.genewiz.com.cn/](https://www.genewiz.com.cn/). The final 84 non-redundant TFs/TRs interacting with PtDAL10 were showed in the paper. We put all the potential interaction factors of DAL10 in this table, and annotated the function of each gene. **The meaning of the headers in the data set is as follows:** Target_id, refers to the numbered locus of the Chinese pine gene. est_counts-1, is the number of reads of the interacting genes screened in the first repeated experiment, (the higher it is, the more times it has been screened). Est_counts-2, is the number of reads of the interacting genes screened in the second repeated experiment. Gene ID, refers to the name of the Chinese pine gene. Function description, refers to the corresponding function of the Chinese pine gene. Function, refers to the category of the Chinese pine gene, which is divided into TF (transcription factor) and TR (transcription regulation factor). Pathway, refers to the development regulatory pathway to which the Chinese pine gene belongs. Arabidopsis_homolog, refers to the locus number of the most similar gene in Arabidopsis of the Chinese pine gene. At_Evalue, refers to the Evalue value of the blast of the Chinese pine gene and the homologous gene in Arabidopsis. Arabidopsis_gene_symbol, refers to the name of the most similar gene in Arabidopsis of the Chinese pine gene. "_", is a placeholder to replace the space in the file operation and has no actual meaning."