cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/jy0-cqf-eet
title: Oliva reticulata (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/q7h-r12-0en
title: Merica melanostoma (G. B. Sowerby II, 1849)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gdt-0zy-h41
title: Retusa umbilicata (Montagu, 1803)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/b23-k7d-qrx
title: Gastropoda Cuvier, 1795

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/h4b-rbl-tpr
title: Conus inscriptus Reeve, 1843

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/pbm-6q7-3zm
title: Conus generalis Linnaeus, 1767

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yzw-qwk-xn1
title: Turbonilla Risso, 1826

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/2qg-3np-gdd
title: Ithycythara Woodring, 1928

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/bze-pme-cc3
title: Weinkauffia turgidula (Forbes, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/88b-gjh-ylz
title: Nassarius fenistratus (Marrat, 1877)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gg2-z4h-nl4
title: Bostryx solutus (Troschel, 1847)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/61h-pmn-qkr
title: Pleurotomella A. E. Verrill, 1872

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/86l-1cj-4ys
title: Turridae H. Adams & A. Adams, 1853 (1838)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/d7l-a3f-3vk
title: Penion sulcatus (Lamarck, 1816)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.15468/dl.gwu4x4
title: Occurrence Download

description: A dataset containing 10 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Jassa marmorata",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 10 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009486-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/c73-cmg-epg
title: Oliva vidua (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gw3-kej-yqt
title: Ersilia mediterranea (Monterosato, 1869)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fwc-zts-3fp
title: Pseudofusus pulchellus (R. A. Philippi, 1840)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ty5-8x8-2ch
title: Jaspidella jaspidea (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/eds-rw7-tvv
title: Olivella olssoni van Regteren Altena, 1971

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/rrz-g3y-5mq
title: Amoria maculata (Swainson, 1822)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4sa-ave-e0n
title: Crisilla depicta (Manzoni, 1868)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/tja-3nw-ngx
title: Janthina exigua Lamarck, 1816

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/cz8-hq9-gfa
title: Tritia mutabilis (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/eat-zvh-t2w
title: Alia carinata (Hinds, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s5l-bs8-gvw
title: Bullia diluta (Krauss, 1848)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hch-70p-7a2
title: Pyramidellidae J. E. Gray, 1840

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/kyy-63h-qe4
title: Crassispira fuscescens (Reeve, 1843)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/710-0q6-jl8
title: Williamia gussoni (O. G. Costa, 1829)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/nj4-tj5-8zl
title: Mitrella lincolnensis (Reeve, 1859)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s8x-aks-276
title: Chrysallida pyrgulina Peñas & Rolán, 1998

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9hk-7m5-w3a
title: Tritia reticulata (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vc0-xv8-zqb
title: Oliva carneola (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jjk-n3r-kd2
title: Isara glabra (Swainson, 1821)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/xmy-26c-qzy
title: Scalptia foveolata (G. B. Sowerby II, 1849)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/adc-2fv-gyv
title: Tritia incrassata (Strøm, 1768)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/sew-geh-49b
title: Onoba moreleti Dautzenberg, 1889

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.15468/dl.k7jadv
title: Occurrence Download

description: A dataset containing 36 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Jassa marmorata",
 "Country is Chile",
 "HasCoordinate is true"
 ]
}

 The dataset includes 36 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009487-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/vx9-vcs-v5s
title: Turbonilla pusilla (R. A. Philippi, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9lt-gny-rd4
title: Spiralina alpinoligustica (Sacco, 1892)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/exe-q40-dk7
title: Parthenina interstincta (J. Adams, 1797)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/8sb-dk3-ys0
title: Retusidae Thiele, 1925

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s9j-lgg-gk4
title: Mitrella nympha (Kiener, 1841)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/lw5-jbx-ynv
title: Cylichna Lovén, 1846

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/nhr-7fq-cha
title: Marginella cleryi Petit de la Saussaye, 1836

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/pyt-leb-abp
title: Nassarius capensis (Dunker, 1846)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/3y6-wnk-v2s
title: Duplicaria ustulata (Deshayes, 1857)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/7g5-ryz-mdr
title: Leucotina A. Adams, 1860

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0zz-fwm-k6a
title: Cantharus spiralis Gray, 1839

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ckz-bl4-fdv
title: Conus litteratus Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/tcx-03x-nqm
title: Conus hieroglyphus Duclos, 1833

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/3jdcpr5mb2
title: Jing manuscript original data_ Visualization of CYP3A4 Expression at both mRNA and Protein Levels Simultaneously at Single Cell Resolution in Various Conditions Using RNAscope Combined with Immunofluorescence

description: Research Hypothesis and Summary

We hypothesized that CYP3A4 expression is dynamically regulated at both mRNA and protein levels during hepatic differentiation and under various treatment conditions, and that a combined RNAscope and immunofluorescence (IF) approach would enable simultaneous, spatially resolved detection of CYP3A4 transcription and translation at single-cell resolution.

To test this, we employed a dual-modality imaging method using RNAscope for mRNA detection and IF for protein visualization in HepaRG cells across four time points (day 7, 14, 21, 28) and under five treatment conditions (glucose, FFAs, rifampicin, APAP, control). Two quantification strategies were used: whole-field intensity to mimic bulk assays (RT-qPCR, WB, FC, ELISA), and cell-specific intensity in CYP3A4-positive cells.

Key Findings

Cell Model Comparison: Among three hepatic models, only differentiated HepaRG cells robustly expressed CYP3A4 in HLCs, with expression absent in CLCs, HepG2, and Huh7 cells.

Differentiation Dynamics: CYP3A4 mRNA levels gradually increased in whole-field measurements but did not show significant differences between adjacent time points. Protein levels significantly increased from day 14 onward. In positive cells, mRNA increased progressively through day 28, while protein levels plateaued after day 14.

Single-Cell Patterns: Five distinct CYP3A4 expression patterns were identified, reflecting different stages from transcription initiation to translation and downregulation. These patterns revealed asynchronous mRNA-protein expression in individual cells.

Treatment Effects:

Glucose (50 mM): No effect on mRNA or protein levels.

FFAs (500/1000 µM): Both doses reduced CYP3A4 mRNA and protein levels; higher FFA caused a greater reduction in mRNA.

Rifampicin (10 µM): Significantly increased mRNA, but not protein levels.

APAP (10 mM): Decreased both mRNA and protein, with visible loss of CYP3A4-positive HLCs likely due to selective cytotoxicity.

Interpretation and Application

This study demonstrates that CYP3A4 expression is regulated in a cell-type- and condition-specific manner, with transcription and translation not always aligned. The combined RNAscope-IF technique allows dual detection of CYP3A4 mRNA and protein within the same cells, offering both spatial and quantitative insights. This platform provides a valuable tool for studying hepatic functional heterogeneity, xenobiotic metabolism, and drug-induced toxicity at the single-cell level, and can inform preclinical drug screening, toxicology, and pharmacokinetic modeling.
DOI: 10.3535/p45-11b-8np
title: Conus eburneus Hwass, 1792

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/sej-5l6-wjy
title: Olivella Swainson, 1831

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/p9v-f69-b54
title: Conus lividus Hwass, 1792

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vhs-rht-nc3
title: Conus lischkeanus Weinkauff, 1875

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/3jdcpr5mb2.1
title: Jing manuscript original data_ Visualization of CYP3A4 Expression at both mRNA and Protein Levels Simultaneously at Single Cell Resolution in Various Conditions Using RNAscope Combined with Immunofluorescence

description: Research Hypothesis and Summary

We hypothesized that CYP3A4 expression is dynamically regulated at both mRNA and protein levels during hepatic differentiation and under various treatment conditions, and that a combined RNAscope and immunofluorescence (IF) approach would enable simultaneous, spatially resolved detection of CYP3A4 transcription and translation at single-cell resolution.

To test this, we employed a dual-modality imaging method using RNAscope for mRNA detection and IF for protein visualization in HepaRG cells across four time points (day 7, 14, 21, 28) and under five treatment conditions (glucose, FFAs, rifampicin, APAP, control). Two quantification strategies were used: whole-field intensity to mimic bulk assays (RT-qPCR, WB, FC, ELISA), and cell-specific intensity in CYP3A4-positive cells.

Key Findings

Cell Model Comparison: Among three hepatic models, only differentiated HepaRG cells robustly expressed CYP3A4 in HLCs, with expression absent in CLCs, HepG2, and Huh7 cells.

Differentiation Dynamics: CYP3A4 mRNA levels gradually increased in whole-field measurements but did not show significant differences between adjacent time points. Protein levels significantly increased from day 14 onward. In positive cells, mRNA increased progressively through day 28, while protein levels plateaued after day 14.

Single-Cell Patterns: Five distinct CYP3A4 expression patterns were identified, reflecting different stages from transcription initiation to translation and downregulation. These patterns revealed asynchronous mRNA-protein expression in individual cells.

Treatment Effects:

Glucose (50 mM): No effect on mRNA or protein levels.

FFAs (500/1000 µM): Both doses reduced CYP3A4 mRNA and protein levels; higher FFA caused a greater reduction in mRNA.

Rifampicin (10 µM): Significantly increased mRNA, but not protein levels.

APAP (10 mM): Decreased both mRNA and protein, with visible loss of CYP3A4-positive HLCs likely due to selective cytotoxicity.

Interpretation and Application

This study demonstrates that CYP3A4 expression is regulated in a cell-type- and condition-specific manner, with transcription and translation not always aligned. The combined RNAscope-IF technique allows dual detection of CYP3A4 mRNA and protein within the same cells, offering both spatial and quantitative insights. This platform provides a valuable tool for studying hepatic functional heterogeneity, xenobiotic metabolism, and drug-induced toxicity at the single-cell level, and can inform preclinical drug screening, toxicology, and pharmacokinetic modeling.
DOI: 10.3535/d9q-7d4-e91
title: Conus asiaticus da Motta, 1985

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/sn2-880-9s0
title: Cystiscidae W. Stimpson, 1865

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/96a-yt4-7q2
title: Xanthodaphne heterogramma (Odhner, 1960)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/47z-g0v-fnw
title: Terebra lima Deshayes, 1857

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/451-1r9-8vj
title: Psilaxis radiatus (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/knx-jnb-em3
title: Ringicula Deshayes, 1838

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/9sykj5s75c
title: Demographic Factors Affect the BMAC Proteome Dataset

description: CSV file is the result of each text file. 
 
Wet work: BMAC samples were spun to searate out cellular and serum component. The serum was then filtered through an IgG and albumin exclusion column before performing a bicinchoninic acid assay to determine each samples concentration. All samples were diluted to a uniform concentration and applied to one of four antibody-microarrays. 

Data processing: Microarrays were scanned in GenePix4000b scanner using the GenePix Pro 6.0.1.25 software. Raw data was exported and the 'F52 Mean- - B532' data was used. Proteins were analyzed in quadruplicate, all spots greater than 20% of the median value of their sister spots were excluded. Spots which met the inclusion criteria were averaged and a log2 transform was applied before normalizing all spots to the average of two control samples run on each array. 

*Note features in excel file have been obfuscated, all values remain, but names have be anonymized for future publication purposes*
DOI: 10.3535/38z-64m-jzf
title: Nassarius scissuratus (Dall, 1889)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/bqs-wzb-r6d
title: Architectonica perspectiva (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hzl-hw8-4fd
title: Ringicula Deshayes, 1838

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/9sykj5s75c.1
title: Demographic Factors Affect the BMAC Proteome Dataset

description: CSV file is the result of each text file. 
 
Wet work: BMAC samples were spun to searate out cellular and serum component. The serum was then filtered through an IgG and albumin exclusion column before performing a bicinchoninic acid assay to determine each samples concentration. All samples were diluted to a uniform concentration and applied to one of four antibody-microarrays. 

Data processing: Microarrays were scanned in GenePix4000b scanner using the GenePix Pro 6.0.1.25 software. Raw data was exported and the 'F52 Mean- - B532' data was used. Proteins were analyzed in quadruplicate, all spots greater than 20% of the median value of their sister spots were excluded. Spots which met the inclusion criteria were averaged and a log2 transform was applied before normalizing all spots to the average of two control samples run on each array. 

*Note features in excel file have been obfuscated, all values remain, but names have be anonymized for future publication purposes*
DOI: 10.3535/9zl-9m7-vh0
title: Brachystomia eulimoides (Hanley, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ps2-gnf-rdk
title: Turbonilla Risso, 1826

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4af-0vt-xeq
title: Strigatella scutulata (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/5hk-lcv-1dp
title: Conus magus Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ppe-vgn-dev
title: Oliva vidua (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/6vb-8av-mjb
title: Costellariidae MacDonald, 1860

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/83l-cpr-r73
title: Conus aemulus Reeve, 1844

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qq3-5qk-tct
title: Tritia varicosa (W. Turton, 1825)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/lwk-g2e-w1d
title: Acteonidae A. d'Orbigny, 1842

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/y5k-t6p-gbf
title: Marginella cleryi Petit de la Saussaye, 1836

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/53l-21x-s3f
title: Granulina guancha (A. d'Orbigny, 1840)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/2tncgrz4hh
title: Safety and efficacy of topical imiquimod use in allogeneic hematopoietic stem cell transplant recipients: a multicenter retrospective analysis

description: Supplemental Table 1. Patient demographics and details of allogeneic HSCT for the patient cohort. 
Supplemental Table 2: Response rates and recurrence rates across various indications after imiquimod treatment in allo-HSCT recipients.
DOI: 10.3535/40e-tpw-xc4
title: Ericusa fulgetrum (G. B. Sowerby I, 1825)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/7pa-je6-gqr
title: Cylichnidae H. Adams & A. Adams, 1854

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/2tncgrz4hh.1
title: Safety and efficacy of topical imiquimod use in allogeneic hematopoietic stem cell transplant recipients: a multicenter retrospective analysis

description: Supplemental Table 1. Patient demographics and details of allogeneic HSCT for the patient cohort. 
Supplemental Table 2: Response rates and recurrence rates across various indications after imiquimod treatment in allo-HSCT recipients.
DOI: 10.3535/3l7-vpn-xxn
title: Tritia denticulata (A. Adams, 1852)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/953-adg-gwh
title: Tritia corniculum (Olivi, 1792)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/t4j-aev-ejw
title: Hastula hectica (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/w40-jn3-af4
title: Nassarius splendidulus (Dunker, 1846)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/5l1-pl1-8a0
title: Strioturbonilla sigmoidea (Monterosato, 1880)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gmb-rf2-1wm
title: Vexillum oleaceum (Reeve, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qm4-vty-mra
title: Nassarius vinctus (Marrat, 1877)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/1q0-lrm-g4r
title: Conus marmoreus Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/d9g-g5l-bkc
title: Austroginella johnstoni (Petterd, 1884)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.