DOI: 10.5061/dryad.gs45f
title: Data from: Herbivory-induced volatiles function as defenses increasing fitness of the native plant Nicotiana attenuata in nature

description: From an herbivore’s first bite, plants release herbivory-induced plant
 volatiles (HIPVs) which can attract enemies of herbivores. However, other
 animals and competing plants can intercept HIPVs for their own use, and it
 remains unclear whether HIPVs serve as an indirect defense by increasing
 fitness for the emitting plant. In a two-year field study, HIPV-emitting
 N. attenuata plants produced twice as many buds and flowers as
 HIPV-silenced plants, but only when native Geocoris spp. predators reduced
 herbivore loads (by 50%) on HIPV-emitters. In concert with HIPVs, plants
 also employ antidigestive trypsin protease inhibitors (TPIs), but
 TPI-producing plants were not fitter than TPI-silenced plants. TPIs
 weakened a specialist herbivore’s behavioral evasive responses to
 simulated Geocoris spp. attack, indicating that TPIs function against
 specialists by enhancing indirect defense.
description: F1A_SchumanBarthelBaldwin2012TPIactivityTrypsin protease inhibitor activity in systemic leaves of herbivore-attacked plants to accompany Figure 1A.F1B_SchumanBarthelBaldwin2012PItranscriptsQuantitative PCR (SYBR GREEN) data and analysis of PI gene transcript abundance, to accompany Figure 1B.F2A_SchumanBarthelBaldwin2012chromatogramsText files of GC-MS chromatograms shown in Figure 2A.F2B_SchumanBarthelBaldwin2012chromatogramsText files of GC-FID chromatograms shown in Figure 2B.F3A_SchumanBarthelBaldwin2012GLVpoolsGC-FID peak areas and data analysis to accompany Figure 3A.F3B_SchumanBarthelBaldwin2012GLVheadspaceGC-MS and Z-Nose (tm) peak areas and data analysis to accompany Figure 3B.F3C_SchumanBarthelBaldwin2012LOX2transcriptsQuantitative PCR (SYBR Green) data and analysis of LOX2 gene transcript abundance to accompany Figure 3C.F5BC_SchumanBarthelBaldwin2012predationLarval and egg predation data and analysis to accompany Figure 5 B and C.F6AB_SchumanBarthelBaldwin2012growth_reproduction2011Growth and reproduction data from plants in the 2011 field experiment to accompany Figure 6 A and B.F6AC_SchumanBarthelBaldwin2012growth_reproduction2010Growth and reproduction data from plants in the 2010 field experiment to accompany Figure 6 A and C.F7A_SchumanBarthelBaldwin2012herbivoreDamage2010Herbivore damage data and analysis for plants in the 2010 field experiment, to accompany Figure 7A.F7BC_SchumanBarthelBaldwin2012herbivoreDamage2011Herbivore damage data and analysis for plants in the 2011 field experiment, to accompany Figure 7 B and C.F8_SchumanBarthelBaldwin2012tripletsGrowth, reproduction, herbivore damage and health data and analysis from plants matched for assay M4 (see timeline in Figure 1 of the publication), to accompany Figure 8.F9A_SchumanBarthelBaldwin2012data2010Manduca spp. larval mortality and plant reproduction data and analysis from plants in the 2010 field experiment, to accompany Figure 9A.F9B_SchumanBarthelBaldwin2012data2011Manduca spp. larval mortality and plant reproduction data and analysis for plants in the 2011 field experiment, to accompany Figure 9B.F10_SchumanBarthelBaldwin2012ManducaManduca spp. behavior and performance data and analysis, to accompany Figure 10.T3_SchumanBarthelBaldwin2012growth_reproduction2011Additonal analysis of the growth and reproduction data for plants in the 2011 field experiment, to accompany Table 4; referred to in the caption for Figure 6.Video-1On-plant assay, plant 7u, WT, June 18, 2011. H.264/MPEG-4 AVC video.Video-2On-plant assay, plant 2o, irPI, June 18, 2011. H.264/MPEG-4 AVC video.Video-3Off-plant assay, replicate 3, WT, June 24, 2011. H.264/MPEG-4 AVC video.Video-4Off-plant assay, replicate 3, irPI, 24th June 2011. H.264/MPEG-4 AVC video.