cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7105796
title: Impaired copper transport in schizophrenia results in a copper-deficient brain state: A new side to the dysbindin story

description: <b>Objectives: </b>Several schizophrenia brain regions exhibit decreased dysbindin. Dysbindin modulates copper transport crucial for myelination, monoamine metabolism and cellular homeostasis. Schizophrenia patients (SZP) exhibit increased plasma copper, while copper-decreasing agents produce schizophrenia-like behavioural and pathological abnormalities. Therefore, we sought to determine dysbindin and copper transporter protein expression and copper content in SZP. <b>Methods: </b>We studied the copper-rich substantia nigra (SN) using Western blot and inductively-coupled plasma mass spectrometry. We characterised specific protein domains of copper transporters ATP7A, CTR1, ATP7B and dysbindin isoforms 1 A and 1B/C in SZP (<i>n</i> = 15) and matched controls (<i>n</i> = 11), and SN copper content in SZP (<i>n</i> = 14) and matched controls (<i>n</i> = 11). As a preliminary investigation, we compared medicated (ON; <i>n</i> = 11) versus unmedicated SZP (OFF; <i>n</i> = 4). <b>Results: </b>SZP exhibited increased C terminus, but not N terminus, ATP7A. SZP expressed less transmembrane CTR1 and dysbindin 1B/C than controls. ON exhibited increased C terminus ATP7A protein versus controls. OFF exhibited less N terminus ATP7A protein than controls and ON, suggesting medication-induced rescue of the ATP7A N terminus. SZP exhibited less SN copper content than controls. <b>Conclusions: </b>These results provide the first evidence of disrupted copper transport in schizophrenia SN that appears to result in a copper-deficient state. Furthermore, copper homeostasis may be modulated by specific dysbindin isoforms and antipsychotic treatment.
DOI: 10.6084/m9.figshare.7105685
title: Ferroxidase activity assays on Rhodospirillum rubrum, Pyrococcus furiosus, and Haliangium ochraceum Encapsulated ferritins

description: Ferritin ferroxidase centre (FOC) catalytically converts Fe(II) into less toxic Fe(III). Ferroxidase assays were conducted to detect formation of iron(III) species at 315 nm by UV-vis spectroscopy. Moreover, inhibition of the enzymatic reaction by addition of competitive Zn(II) was tested in the same way.Protein samples (10 uM) were prepared by diluting purified protein fractions in 10 mM Hepes pH 8.0, 150 mM NaCl under anaerobic conditions. Oxygen-free aliquots of 1mM Fe(II) and Zn(II) were prepared by anaerobically dissolving FeSO4.7H2O or ZnSO4 in 0.1% (v/v) HCl, respectively.Metal and protein samples were then added to a quartz cuvette, aerobically, at the final concentration of 10 uM (protein), 50 uM (Fe(II)), and various Zn(II) concentrations (0, 10, 25, 30, 40, 50, 70 ,100 uM). Absorbance at 315 nm was monitored by UV-vis spectrophotometer every seconds for 1500 s by TimeDrive software. Negative controls were also included, assaying 50 uM Fe(II) in the absence of proteins to test background oxidation, and with each protein plus 100 uM Zn(II) to test the maximum concentration of zinc used i the assay.Data presented here represents three or more technical replicates per condition, means and standard deviations.Data from zinc inhibition experiments were also fitted using a Dose-Response (log(inhibitor) vs. response (three parameters) equation by GraphPad software to calculate IC50 and logIC50 values. Average of three or more technical replicates per condition was used as response.
DOI: 10.6084/m9.figshare.7105655
title: Shared ancestry analysis, TGP

description: Cumulative coalescent function (CCF) profiles between every pair of haploid genomes in data from the 1000 Genomes Project (TGP). Profiles were inferred for Chromosome 20.<br><b>See README for instructions.</b>
DOI: 10.6084/m9.figshare.7105685.v1
title: Ferroxidase activity assays on Rhodospirillum rubrum, Pyrococcus furiosus, and Haliangium ochraceum Encapsulated ferritins

description: Ferritin ferroxidase centre (FOC) catalytically converts Fe(II) into less toxic Fe(III). Ferroxidase assays were conducted to detect formation of iron(III) species at 315 nm by UV-vis spectroscopy. Moreover, inhibition of the enzymatic reaction by addition of competitive Zn(II) was tested in the same way.Protein samples (10 uM) were prepared by diluting purified protein fractions in 10 mM Hepes pH 8.0, 150 mM NaCl under anaerobic conditions. Oxygen-free aliquots of 1mM Fe(II) and Zn(II) were prepared by anaerobically dissolving FeSO4.7H2O or ZnSO4 in 0.1% (v/v) HCl, respectively.Metal and protein samples were then added to a quartz cuvette, aerobically, at the final concentration of 10 uM (protein), 50 uM (Fe(II)), and various Zn(II) concentrations (0, 10, 25, 30, 40, 50, 70 ,100 uM). Absorbance at 315 nm was monitored by UV-vis spectrophotometer every seconds for 1500 s by TimeDrive software. Negative controls were also included, assaying 50 uM Fe(II) in the absence of proteins to test background oxidation, and with each protein plus 100 uM Zn(II) to test the maximum concentration of zinc used i the assay.Data presented here represents three or more technical replicates per condition, means and standard deviations.Data from zinc inhibition experiments were also fitted using a Dose-Response (log(inhibitor) vs. response (three parameters) equation by GraphPad software to calculate IC50 and logIC50 values. Average of three or more technical replicates per condition was used as response.
DOI: 10.6084/m9.figshare.7105655.v1
title: Shared ancestry analysis, TGP

description: Cumulative coalescent function (CCF) profiles between every pair of haploid genomes in data from the 1000 Genomes Project (TGP). Profiles were inferred for Chromosome 20.<br><b>See README for instructions.</b>
DOI: 10.6084/m9.figshare.7105613
title: Shared ancestry analysis, SGDP

description: Cumulative coalescent function (CCF) profiles between every pair of haploid genomes in data from the Simons Genome Diversity Project (SGDP). Profiles were inferred separately for each chromosome, and aggregated by diploid individual, as well as by SGDP population group.<br><b>See README for instructions.</b>
DOI: 10.6084/m9.figshare.7105613.v1
title: Shared ancestry analysis, SGDP

description: Cumulative coalescent function (CCF) profiles between every pair of haploid genomes in data from the Simons Genome Diversity Project (SGDP). Profiles were inferred separately for each chromosome, and aggregated by diploid individual, as well as by SGDP population group.<br><b>See README for instructions.</b>
DOI: 10.15161/oar.it/30651
title: CovidStat project data

description: This record contains the daily updated data of the CovidStat project. CovidStat is a project carried out by the CovidStat Working Group at INFN, whose creation was promoted within the Italian National Institute of Nuclear Physics with the aim of making a statistical analysis of the data provided daily by the Civil Protection on the spread of the pandemic in Italy. CovidStat data can interactively be browsed at https://covid19.infn.it.
description: The software to process this Root TTree is in the related identifier. Be sure to use the right corresponding version, based on the data in which Root TTree was generated.
DOI: 10.6084/m9.figshare.7105433
title: Supplementary Material for: lncRNA DLG1-AS1 Promotes Cell Proliferation by Competitively Binding with miR-107 and Up-Regulating ZHX1 Expression in Cervical Cancer

description: <b><i>Background/Aims:</i></b> Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in the occurrence and development of various tumors, thereby attracting increasing attention from researchers. The important biological functions of lncRNAs have been recognized gradually, but their mechanism in cervical cancer remains unclear. <b><i>Methods:</i></b> Differentially expressed lncRNAs in cervical cancer and para-carcinoma tissues were identified by screening using an lncRNA array, and candidate lncRNAs were verified by quantitative real-time PCR. A series of bioinformatics and molecular biological methods were adopted to investigate the interactions among lncRNAs, microRNAs (miRNAs), and miRNA target genes in cervical cancer. Cell viability was measured using a Cell Counting Kit-8 assay. <b><i>Results:</i></b> DLG1-AS1 was the most significantly up-regulated lncRNA in cervical cancer tissues, and it was confirmed that cervical cancer patients with high DLG1-AS1 expression had a poor prognosis. Down-regulation of DLG1-AS1 expression suppressed the proliferation of cervical cancer cells. Further investigation revealed that DLG1-AS1 eliminated the inhibition of miR-107 on the expression of its target gene ZHX1 by competitively binding to miR-107. Moreover, rescue assays proved that the effect of DLG1-AS1 on the proliferation of cervical cancer cells was dependent on miR-107. <b><i>Conclusion:</i></b> DLG1-AS1/miR-107/ZHX1 can form a competitive endogenous RNA network that regulates the proliferation of cervical cancer cells, resulting in tumor progression.
DOI: 10.6084/m9.figshare.7105211
title: Supplementary Material for: Using the Kinetic Estimating Glomerular Filtration Rate Equation for Estimating Glomerular Filtration Rate and Detecting Acute Kidney Injury: A Pilot Study

description: <b><i>Background:</i></b> Estimating the glomerular filtration rate (eGFR) when the creatinine (Cr) is rapidly changing, as in acute kidney injury (AKI), has been a challenge. The Kinetic Estimated Glomerular Filtration Rate (KeGFR) formula by S. Chen estimates the GFR in the acute state by factoring the time interval between rising Cr values and the volume of distribution (V<sub>D</sub>). It provides the clinician with an eGFR value for each non-steady state Cr value. We applied the KeGFR formula to detect AKI in an adult non-ICU inpatient setting. We then compared KeGFR with the current standard Acute Kidney Injury Network (AKIN criteria) and Risk Injury Failure Loss End-Stage Kidney Disease (RIFLE criteria) and new criteria (Waikar-Bonventre, Delta check) for AKI detection. <b><i>Methods:</i></b> A total of 250 consecutive adult patients admitted to the Medical wards were screened. Patient episodes with a change in Cr of &gt; 4.3% (Biological Variation) were included in the study (<i>n</i> = 80). The KeGFR equation was applied to this cohort after calculating the V<sub>D</sub> individually after estimating the initial GFR by using MDRD equation. A fall in KeGFR of 25% or more was considered as AKI. The AKIN, Waikar-Bonventre, RIFLE, and Delta Check criteria were also applied to this cohort and compared with the KeGFR criterion. Clinical adjudication was performed when there was discordance between AKI episodes detected by AKIN and KeGFR criteria. <b><i>Results:</i></b> There were 50 episodes of AKI by AKIN classification and 31 episodes by KeGFR criterion. All but 1 (30) episode detected by KeGFR criterion fulfilled the AKI definition by AKIN. AKIN diagnosed an additional 20 episodes. However, all of these had an elevated Cr level on admission; thus, requiring the incorporation of baseline Cr by AKIN which is not part of the KeGFR formula. Five of these 20 patients were deemed as not having AKI by clinical adjudication. All patients with in-hospital AKI and ongoing AKI were detected by both the criteria. The KeGFR criteria detected almost all (24/25) episodes of AKI identified by the Waikar-Bonventre method. The latter method detected 77% (24/31) of the AKI episodes identified by KeGFR. <b><i>Conclusion:</i></b> The KeGFR equation can be readily applied to estimate GFR in the non-steady state. A KeGFR-based criterion successfully detected ongoing and in-hospital AKI in this study. Community acquired AKI that did not progress after admission was not detected by the KeGFR criterion.
DOI: 10.6084/m9.figshare.7105211.v1
title: Supplementary Material for: Using the Kinetic Estimating Glomerular Filtration Rate Equation for Estimating Glomerular Filtration Rate and Detecting Acute Kidney Injury: A Pilot Study

description: <b><i>Background:</i></b> Estimating the glomerular filtration rate (eGFR) when the creatinine (Cr) is rapidly changing, as in acute kidney injury (AKI), has been a challenge. The Kinetic Estimated Glomerular Filtration Rate (KeGFR) formula by S. Chen estimates the GFR in the acute state by factoring the time interval between rising Cr values and the volume of distribution (V<sub>D</sub>). It provides the clinician with an eGFR value for each non-steady state Cr value. We applied the KeGFR formula to detect AKI in an adult non-ICU inpatient setting. We then compared KeGFR with the current standard Acute Kidney Injury Network (AKIN criteria) and Risk Injury Failure Loss End-Stage Kidney Disease (RIFLE criteria) and new criteria (Waikar-Bonventre, Delta check) for AKI detection. <b><i>Methods:</i></b> A total of 250 consecutive adult patients admitted to the Medical wards were screened. Patient episodes with a change in Cr of &gt; 4.3% (Biological Variation) were included in the study (<i>n</i> = 80). The KeGFR equation was applied to this cohort after calculating the V<sub>D</sub> individually after estimating the initial GFR by using MDRD equation. A fall in KeGFR of 25% or more was considered as AKI. The AKIN, Waikar-Bonventre, RIFLE, and Delta Check criteria were also applied to this cohort and compared with the KeGFR criterion. Clinical adjudication was performed when there was discordance between AKI episodes detected by AKIN and KeGFR criteria. <b><i>Results:</i></b> There were 50 episodes of AKI by AKIN classification and 31 episodes by KeGFR criterion. All but 1 (30) episode detected by KeGFR criterion fulfilled the AKI definition by AKIN. AKIN diagnosed an additional 20 episodes. However, all of these had an elevated Cr level on admission; thus, requiring the incorporation of baseline Cr by AKIN which is not part of the KeGFR formula. Five of these 20 patients were deemed as not having AKI by clinical adjudication. All patients with in-hospital AKI and ongoing AKI were detected by both the criteria. The KeGFR criteria detected almost all (24/25) episodes of AKI identified by the Waikar-Bonventre method. The latter method detected 77% (24/31) of the AKI episodes identified by KeGFR. <b><i>Conclusion:</i></b> The KeGFR equation can be readily applied to estimate GFR in the non-steady state. A KeGFR-based criterion successfully detected ongoing and in-hospital AKI in this study. Community acquired AKI that did not progress after admission was not detected by the KeGFR criterion.
DOI: 10.6084/m9.figshare.7105205
title: Table S1: List of 126 genes Hac1-dependant genes, no longer upregulated during UPR when HAC1 is deleted

description: List of genes hac1-dependant in the yeast <i>Candida parapsilosis.</i>The data have been generated after RNA-seq on wild type <i>C. parapsilosis</i> and hac1-/- mutant <i>C. parapsilosis </i>exposed to DTT (or to water as a control) for 1h.
DOI: 10.6084/m9.figshare.7105205.v1
title: Table S1: List of 126 genes Hac1-dependant genes, no longer upregulated during UPR when HAC1 is deleted

description: List of genes hac1-dependant in the yeast <i>Candida parapsilosis.</i>The data have been generated after RNA-seq on wild type <i>C. parapsilosis</i> and hac1-/- mutant <i>C. parapsilosis </i>exposed to DTT (or to water as a control) for 1h.
DOI: 10.6084/m9.figshare.7105166
title: Kalinkat, Schneider et al 2013 data

description: Data on a series of laboratory feeding experiments with ground beetles, cursorial spiders and centipedes of different sizes as predators. Each trial contained one predator individual and varying densities of a single prey species of different body sizes.
DOI: 10.6084/m9.figshare.7105181
title: Supplementary Material for: Non-Antibiotic Herbal Therapy (BNO 1045) versus Antibiotic Therapy (Fosfomycin Trometamol) for the Treatment of Acute Lower Uncomplicated Urinary Tract Infections in Women: A Double-Blind, Parallel-Group, Randomized, Multicentre, Non-Inferiority Phase III Trial

description: <b><i>Introduction:</i></b> This randomized, controlled, Phase III non-inferiority clinical trial aimed to determine whether herbal therapy with Canephron® N (BNO 1045) is non-inferior to fosfomycin trometamol (FT) in treating acute lower uncomplicated urinary tract infections (uUTIs). <b><i>Materials and Methods:</i></b> Women aged 18–70 years with typical symptoms of newly diagnosed acute lower uUTIs were randomized to BNO 1045 (<i>n</i> = 325) or FT (<i>n</i> = 334), with corresponding matched placebo. The primary endpoint was the proportion of patients who received additional antibiotics (ABs) to treat uUTIs between Days 1 and 38 ±3. <b><i>Results:</i></b> Between Days 1 and 38, 238 (83.5%) patients in the BNO 1045 group and 272 (89.8%) patients in the FT group received no additional ABs. At a 15% non-inferiority margin, BNO 1045 was non-inferior to FT in treating uUTIs (non-AB rate difference: –6.26%; 95% CI –11.99 to –0.53%; 2-sided <i>p</i> = 0.0014). Adverse event rates were similar between groups, with higher rates of gastrointestinal disorders in the FT group and pyelonephritis in the BNO 1045 group. During the trial, no patient died or discontinued due to a treatment-related adverse event. <b><i>Conclusions:</i></b> BNO 1045 has the potential to reduce outpatient use of ABs for uUTIs and thus may have a significant impact on antimicrobial stewardship strategies. Trial registration: NCT02639520, EudraCT number 2013-004529-99.
DOI: 10.6084/m9.figshare.7105163
title: Tutor of Resilience school-based program in schools affected by the 2016 Italian earthquake

description: Database where we assessed a group of children victims of the 2017 Italian earthquake who took part to a teachers delivered resilience-focused intervention.
DOI: 10.6084/m9.figshare.7105166.v1
title: Kalinkat, Schneider et al 2013 data

description: Data on a series of laboratory feeding experiments with ground beetles, cursorial spiders and centipedes of different sizes as predators. Each trial contained one predator individual and varying densities of a single prey species of different body sizes.
DOI: 10.6084/m9.figshare.7105163.v1
title: Tutor of Resilience school-based program in schools affected by the 2016 Italian earthquake

description: Database where we assessed a group of children victims of the 2017 Italian earthquake who took part to a teachers delivered resilience-focused intervention.
DOI: 10.6084/m9.figshare.7105130
title: Dataset for: Vacuolar sucrose cleavage prevents limitation of cytosolic carbohydrate metabolism and stabilizes photosynthesis under abiotic stress

description: Stabilization of central carbohydrate metabolism plays a key role in plant stress response. Carbohydrates are substrate for numerous metabolic and stress-responsive reactions and have been shown to be involved in diverse signalling processes on a whole plant level. Regulation of enzymatic sucrose synthesis and degradation is well-known to be central to many stress-related processes as it significantly impacts stress tolerance. Leaf sucrose metabolism involves sucrose cleavage by invertases and ATP-consuming re-synthesis catalysed by hexokinase and sucrose phosphate synthase. These reactions establish a metabolic cycle. To study the physiological role of sucrose cycling, a kinetic model was developed to simulate dynamics of subcellular sugar concentrations in Arabidopsis thaliana under combined cold and high light stress. Model simulation revealed that subcellular reprogramming of invertase-driven sucrose cleavage varies substantially between natural accessions of Arabidopsis which differ in their cold tolerance levels. A stress-induced shift of sucrose cleavage from the cytosol into the vacuole could only be observed for the tolerant accession while the susceptible accession increased the cytosolic proportion of sucrose cleavage. Under stress, reduction of vacuolar invertase activity significantly affected maximum quantum yield of photosystem II and CO2 assimilation rates. While wild type plants circumvented a limitation of sucrose cleavage by increasing vacuolar invertase activity, mutant plants were not able to compensate their deficiency of vacuolar by cytosolic activity. Consequently, the capacity for cytosolic hexose generation was lower than for enzymatic hexose phosphorylation suggesting a role of vacuolar invertase activity in preventing a limitation in cytosolic hexose metabolism under stress.
DOI: 10.6084/m9.figshare.7105097
title: FIGURE 13. The patterns, surface elements and wettability on the prepared PAN (a) and PVC (b) nanofibers in the presence inclusions (at 0, 4, 8, 16, and 32wt %).

description: shows the water contact angles of PAN fibers incorporated with 16wt % of PVP polymers at intervals of 0 and 2 seconds
DOI: 10.6084/m9.figshare.7105082
title: Figure 9, 10, 11, Humidifier test DMF in PAN/PVC/PVP, 90:10 at Room Temperature.

description: humidifier test results conducted on PVC nanofibers incorporated with 16wt % of PVP. The moisture gain was relatively high up to 60 minutes of the fog tests and then was reduced considerably.
DOI: 10.6084/m9.figshare.7105097.v1
title: FIGURE 13. The patterns, surface elements and wettability on the prepared PAN (a) and PVC (b) nanofibers in the presence inclusions (at 0, 4, 8, 16, and 32wt %).

description: shows the water contact angles of PAN fibers incorporated with 16wt % of PVP polymers at intervals of 0 and 2 seconds
DOI: 10.6084/m9.figshare.7105079
title: FIGURE 8. FTIR spectra of 32%wt. PEG in PAN and PVP in PAN.

description: Figure 8 shows the<b> </b>FTIR absorption spectra of PAN powder incorporated with 32wt% of PVP powder. The vibration characteristics of this combination are like the organic group equivalent to 1103 cm<sup>-1</sup>.
DOI: 10.6084/m9.figshare.7105082.v1
title: Figure 9, 10, 11, Humidifier test DMF in PAN/PVC/PVP, 90:10 at Room Temperature.

description: humidifier test results conducted on PVC nanofibers incorporated with 16wt % of PVP. The moisture gain was relatively high up to 60 minutes of the fog tests and then was reduced considerably.
DOI: 10.6084/m9.figshare.7105073
title: FIGURE 7. FTIR spectra of 16%wt. Chitosan in PVC, Chitosan in PAN, PVC in PVP.

description: Figure 7<b> </b>shows the FTIR absorption spectra of PAN powder incorporated with 16wt% chitosan powder. The FTIR spectra of this combination provide some nitrile groups, equivalent to 669 cm-1.
DOI: 10.6084/m9.figshare.7105079.v1
title: FIGURE 8. FTIR spectra of 32%wt. PEG in PAN and PVP in PAN.

description: Figure 8 shows the<b> </b>FTIR absorption spectra of PAN powder incorporated with 32wt% of PVP powder. The vibration characteristics of this combination are like the organic group equivalent to 1103 cm<sup>-1</sup>.
DOI: 10.6084/m9.figshare.7105064
title: FIGURE 6. FTIR spectra of PAN, PVC, PVP and Chitosan Powder.

description: Figure 6 shows the FTIR absorption spectra of PVP powder only without any inclusions. The vibration characteristics of the PVP powder resemble with the nitrile group