cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2018.96
title: Comprehensive proteomics identification of IFN-λ3-regulated antiviral proteins in HBV-transfected cells

description: Interferon (IFN)-λ is a relatively unexplored, yet promising anti-viral agent. IFN-λ has recently been tested in clinical trials of chronic hepatitis B, with the advantage that side effects may be limited compared with IFN-α, as IFN-λ receptors are found only in epithelial cells. To date, IFN-λ downstream signaling pathway remains largely unelucidated, particularly via proteomics methods. Here, we report that IFN-λ3 inhibits HBV replication in HepG2.2.15 cells, reducing levels of both HBV transcripts and intracellular HBV DNA. Quantitative proteomic analysis of HBV-transfected cells was performed following 24-hour IFN-λ3 treatment, with parallel IFN-α2a and PBS treatments for comparison using a dimethyl labeling method. The depth of the study allowed us to map the induction of anti-viral proteins to multiple points of the viral life cycle, as well as facilitating the identification of anti-viral proteins not previously known to be elicited upon HBV infection. This study also shows up-regulation of many effectors involved in antigen processing/presentation indicating that this cytokine exerted immunomodulatory effects through a number of essential molecules for these processes. Interestingly, immunoproteasome caps were up-regulated while cap components of the constitutive proteasome were down-regulated upon IFN-λ3 treatment, suggesting coordinated modulation towards the antigen processing/presentation mode. Furthermore, we reveal that IFN-λ3 restored levels of RIG-I and RIG-G, proteins known to be suppressed by HBV. Enrichment analysis demonstrated that several biological processes including RNA metabolism, translation, and ER-targeting were differentially regulated upon treatment with IFN-λ3 vs. IFN-α2a. Our proteomic data suggests that IFN-λ3 regulates an array of cellular processes to control HBV replication.
DOI: 10.14457/cu.the.2018.97
title: Rethinking inclusive and sustainable educational development for Moken children

description: Despite numerous education readjustments, efforts for equal opportunity in education, and an increase in present enrollment, many indigenous children in Thailand continue to fall behind in the formal education system. This study focuses on the Moken Chao Lay (sea people) communities in southern Thailand and looks to analyze and understand situations and shortcomings of Moken children’s education and why they are still underperforming in the formal education system. Traditional ways of knowing, especially knowing through nature, are used as a platform to rethink the way we look at indigenous children’s education in Thailand. Interview data was collected from school teachers, NGOs staff working on children and education, and academics on Moken and other indigenous peoples’ issues as well as from Moken students, community members, and elders in the communities. In addition, observation of Moken daily activities and Moken children in school/learning centers was made during each field trip. The results showed a gap between a wider open policy and practices when it came to “inclusiveness” in formal education, and that Moken and other indigenous learners were still being left behind despite educational readjustments with actual opportunities not matching the perceived opportunities. The review of alternative education movement in Thailand, especially in learning centers in several hill-tribe communities, reveals progressive curriculum and unconventional teaching methods that place a high value on indigenous ecological knowledge. One Moken learning center and two curriculums found in Moken-only schools under this study encourage hands-on learning style, life skills and livelihood skills. This study identifies alternate approaches to sustainable and inclusive education for Moken children in the Thai education context. Traditional practices of knowledge transmission are looked at to help bridge the gap between Moken learners and the formal education system in order contextualize and make relevant the curriculum that is taught. While this study only focuses on the Moken, findings and conclusions may be drawn to encompass a larger scope in terms of implications for other indigenous learners in Thailand.
DOI: 10.14457/cu.the.2018.98
title: HSV-1 induces filopodia formation in activated T lymphocytes

description: Herpes simplex virus type 1 (HSV-1) is a widespread pathogen, which has diverse clinical manifestations from mild localized infection to severe systemic infection. The tissue tropism of HSV-1 is mainly epithelial and mucosal cells. However, it can also multiply in T lymphocytes suggesting a cause of viremia. In this study, CD3+CD4-CD8- Jurkat T lymphocytes were used. Under activation, up-regulation of CD69 molecules on cell surface was observed. Increase yield of HSV-1 production in activated T lymphocytes was demonstrated. In addition, most of HSV-1 virion progenies from infected T lymphocytes were released extracellularly, suggesting the cause of dissemination. The mechanism which supports HSV-1 infection in activated T lymphocytes is unclear. In epithelial cells, HSV-1 induce filopodia formation which constructed by actin polymerization to support their infectivity. Moreover, in those type of cell, filopodia formation are regulated by Cdc42, a member of Rho GTPase protein. In this study, the trend of β-actin mRNA expression was increased after HSV-1 infection in both activated and non-activated cell while the statistically different was found at protein level. β-actin expression was overexpressed after HSV-1 infection in activated T lymphocytes and decreased in cytochalasin D (cyto D), an inhibitor of actin polymerization and depolymerization of actin filaments formed, treated cells. This result indicated that actin plays role during HSV-1 entry in activated T cells. Moreover, Cdc42 was induced by HSV-1 whereas the expression of other Rho GTPase proteins was not changed. During viral entry, filopodia formation was observed in HSV-1-infected activated T lymphocytes suggesting filopodia formation might play role in HSV-1 entry in T-lymphocytes similar to epithelial cells. Observation under electron microscope suggested that HSV-1 entered into activated T lymphocytes via fusion and endocytosis pathway. In addition, HSV-1 titer in cyto D treated activated T lymphocytes was decreased indicating that actin polymerization plays role in HSV-1 infection of activated T lymphocytes. In conclusion, in activated T lymphocytes, filopodia formation, induced by HSV-1 infection was constructed by actin polymerization through Cdc42 which then resulted in increased yield of HSV-1 production.
DOI: 10.14457/cu.the.2018.99
title: The relationship between attitudes toward the Test of English for International Communication (TOEIC) and scores of Thai test takers

description: Attitudes have played a pivotal role affecting not only English language learning but also language test performance. There have been some studies exhibiting that students’ attitudes in English language learning were significantly related to their English test proficiency and their scores. The objectives of this study were to examine Thai test takers’ attitudes toward the Test of English for International Communication (TOEIC) and explored the relationship between Thai test takers’ attitudes toward the TOEIC and their scores. This study used quantitative and qualitative methods to collect and analyze the data under the research framework adapted from the ABC model of Attitudes (Solomon, 2010) and the CAC model (Schiffman and Kanuk, 2004). The participants were 389 Thai TOEIC test takers who were sampled by the purposive sampling technique. Descriptive statistics were used in the analysis of the quantitative part. Ten voluntary participants representing different levels of the English proficiency were interviewed to explore the in-depth information. The qualitative data were analyzed by content analysis. The findings from both qualitative and quantitative data analysis illustrated the Thai test takers’ attitudes toward the Test of English for International Communication (TOEIC) in three aspects that were affective, behavioral, and cognitive components. The overall attitudes of the participants toward the TOEIC test were positive. Moreover, there was a weak significant positive relationship between attitudes and the test takers’ TOEIC scores. Overall, the results of this study will contribute to teaching and learning English, especially for the TOEIC test that will lead test takers to achieve their goal.
DOI: 10.14457/cu.the.2018.100
title: Application of reduced graphene oxide/ natural rubber composite for petroleum removal in sea water

description: In this study, the natural rubber (NR) composite foam was prepared to enhance oil adsorption performance of NR foam. The reduced graphene oxide (rGO) was synthesized from graphite waste by oxidation and reduction processes. In the oxidation step, different oxidation conditions (thermal-sonication and stirring-sonication) were studied in order to achieve the highest interlayer spacing in graphite structure. Then, the reduction was carried out using L-ascorbic acid (L-AA) as a reducing agent. Furthermore, morphology and surface properties of the synthesized rGO were investigated. To prepare the NR composite foams, NR latex was mixed with various contents of rGO (0.25, 0.5, 1.0 and 1.5 parts by weight per hundred part of rubber: phr). The results showed that the amount of rGO affected the morphology and the oil adsorption capacity of NR composite foams. The optimum amount of rGO in the NR composites foam which exhibited the highest oil adsorption performance was 0.5 phr. The oil adsorption capacity of NG-0.5 for gasohol, kerosene, crude oil, diesel and fuel was 21.50, 19.31, 17.04, 16.53 and 10.09 g g-1, respectively. In addition, the effect of temperature and turbulence on the oil adsorption capacity of the NR composite foams was determined. Three kinetic models, i.e. pseudo-first-order, pseudo-second-order and Elovich equation, were examined. The pseudo-second-order presented the better fit with the experimental data. The Langmuir isotherm was an appropriated model to predict the oil adsorption behavior of the NR composite foams. Finally, reusability feature of the NR composite foams was also studied through fifteen oil adsorption-desorption cycles. The result showed that the NR composite foams could be reused in many times.
DOI: 10.14457/cu.the.2018.101
title: Comparative analysis of community solar projects between Thailand and Japan

description: Community-solar (CS) has appeared as a new solar PV ownership model in the word, in which the business model of CS should vary depending on each country’s context. Various unique factors of each country such as the design of the government’s support, characteristics of the community, and business custom may have an impact on how community members benefit from CS projects. The objective of this research is to compare the implemented CS projects in Thailand and Japan and to provide policy recommendation on CS for both countries based on comparison for contribution to further development of CS. The selected case study in this research was conducted in the CS projects at Chon Buri and Prachuap Khiri Khan in Thailand and Kitakyushu and Awaji in Japan. Here, I performed comparative analysis on business models and financial parameters on the current existing two CS projects each in Thailand and Japan considering own energy situation. Also, interviews with the CS projects relevant people were conducted. The economic indicators of CS projects were evaluated by the System Advisor Model, which can calculate net present value, levelized cost of energy (LCOE) and so on. From the data collected through the interviews, it is clarified that the investments in Thailand's CS projects are only about one-third of the investments in Japanese CS projects. The main reasons are the difference in the labor cost, the price of the instruments. As the results of analyses, policy recommendations to both Japanese and Thailand government were made. For the Japanese government, the extension of project lifetime is suggested. Regarding Thailand, FiT price and lifetime should be determined carefully.
DOI: 10.14457/cu.the.2018.102
title: Local knowledge framework for sustainable utilization of mangrove forest : a case study of Klong Khone sub-district, Mueang district, Samut Songkhram province, Thailand

description: A development of local knowledge or local knowledge life cycle for locally appropriate sustainable utilization of mangrove ecosystem services was influenced by factors that were specific to ecological and social-cultural conditions and varied relying a change of the targeted mangrove ecosystem services for utilization. This study aims 1) to identify the factors in the local knowledge life cycle during two phases of mangrove stand initiation and young forest regrowth at Klong Khone sub-district, Samut Songkhram province, Thailand and 2) to propose the local knowledge framework for the sustainable utilization of mangrove ecosystem services and lessons learned through the local knowledge life cycle in the study area. The data was collected by field observation, questionnaires survey totally 160 respondents who were 140 fishermen, and in-depth interview with 20 key stakeholders who had roles and responsibilities involving the local knowledge life cycle during the mangrove forest development. They included former and current village chiefs, local governmental agencies, community enterprises, local philosophers, and mangrove forest conservation groups. Descriptive statistical analysis and event analysis were employed to analyze the collected data. The results showed that the factors in local knowledge life cycle during two phases were ecological, managerial, emotional, and social factors. The ecological factors, especially a reduction of aquatic animals which affected the local people’s income and livelihood motivated the local people’s perception and led them to restore mangrove forest for accruing aquatic animals, solving poverty and sustaining their livelihood. A trust and faith of local people in HRH Princess Maha Chakri Sirindhorn and former village chiefs majorly influenced the local people’s voluntary participation in the local knowledge life cycle during two phases of mangrove forest development. A common property right was a key factor motivating local people’s perception and participation as it indirectly created the local people’s sense of ownership and land tenure and resources security. A mangrove use zoning, and an application of local knowledge and local livelihood also found in a plan validation and monitoring the local people’s utilization of the mangrove ecosystem services. These major factors in the local knowledge life cycle were used to create a local knowledge framework which can be applied for community capacity building to sustain local livelihood under the change of ecological, social, cultural and economic conditions.
DOI: 10.14457/cu.the.2018.103
title: Plastic waste utilization as macro fiber in concrete

description: The use of plastics has increased over the years, thus resulting in a large volume of plastic waste being generated and accumulated in the environment. Due to its non-biodegradability and persistence, recycling processes have become one of the sustainable solutions for preventing environmental deterioration. Plastic wastes, including high density polyethylene (HDPE), low density polyethylene (LDPE), polypropylene (PP), and polyethylene terephthalate (PET), were collected from industrial sector and used as additional ingredients (macro fiber) to improve mortar properties. Prior to mortar processing, an increase in wettability of plastic fibers using nonionic surfactant, Dehydol LS-12, was investigated. At the optimal concentration of 10 times of the critical micelle concentration (CMC), an interfacial tension and a contact angle were reduced to 31–32 mN/m and 65–68 degree, respectively. Properties of synthetic fiber reinforced mortar were determined and compared to those of the conventional mortar samples. Porosity was found to increase ((-8%)-44%) with higher volume fraction of plastic fibers, whereas decreases in workability ((-91%)-52%), bulk density ((-14.7%)-(-0.3%)), thermal conductivity ((-31%)-(-2%)), splitting tensile strength ((-77%)-7%), and compressive strength ((-79%)-(-6%)) were encountered. The lowest thermal conductivity (-31%) was recorded for mortar samples prepared with 30% by volume of LDPE fibers, and the rest in descending order were HDPE, PP, and PET, respectively. The degradations of plastic fibers immersed in alkaline solution (7-180 days) did not reduce their tensile strength significantly while UV-A radiation could decrease their tensile strength with increase time exposure. Furthermore, the maximal inclusions of plastic fibers were 5% for HDPE and LDPE, 10% for PP, and 50% for PET so as to satisfy the precast concrete wall requirements.
DOI: 10.14457/cu.the.2018.104
title: The Role of Cytokine Response Signatures in the Pathogenesis of Leptospirosis Associated Acute Kidney Injury

description: Background: Acute Kidney Injury (AKI) is one of the most serious complications of leptospirosis, an important zoonosis in the tropics. Host pathogen interaction is one of the potential key factor in development of leptospirosis associated AKI. In this multicenter study, we aimed to study the association of cytokines, leptospiral burden, and anti-leptospira antibody and AKI in leptospirosis. Method: In the first cohort, patients who presented with clinical suspiciousness of leptospirosis were prospectively enrolled in 9 centers from August 2012 to November 2014. In the second cohort, we prospectively recruited patients from 15 centers from February 2016 to July 2017. The first day of enrollment was the first day of clinical suspicious leptospirosis. Blood samples were serially collected on the first day and day 7 after enrollment. We used three standard techniques (microscopic agglutination test, direct culture, and PCR technique) to confirm the diagnosis of leptospirosis. KDIGO criteria were used for AKI diagnosis. Results: Of the 206 recruited cases, 113 cases were leptospirosis confirmed cases. Thirty seven percent developed AKI. Median MCP-1 and TNF-a on the first day in those developing AKI were significantly higher than in patients not developing AKI [309.3vs138.8 pg/mL, P = 0.003], and [492.3 vs 77.5 ng/ml, P = 0.003], respectively. MCP-1 and TNF-a levels associated with AKI had AUC-ROC of 0.67, and 0.67, respectively. Only low IL-6 showed strong association with AKI by a covariate-adjusted model. High leptospiral burden and high anti-leptospira antibody associated with severe AKI.Conclusion: From this multicenter study, MCP-1, TNF- a appears to be useful markers for detecting AKI in leptospirosis patients. In the adjusted model for severity, only low IL-6 is associated with AKI. Our data suggest that high leptospiremia and high anti-leptospira antibody correlate with the severity of AKI.
DOI: 10.14457/cu.the.2018.105
title: Molecular Epidemiology And Characterization Of Rotavirus Group A, Group C And Porcine Epidemic Diarrhea Virus In Pigs With Gastroenteritis Among The Commercial Swine Farms In Thailand, 2011-2016

description: The most frequent viruses associated with pig gastroenteritis have been previously reported as porcine epidemic diarrhea virus (PEDV) and rotavirus (RV). Rotavirus is an important cause of diarrhea in piglets and pigs worldwide, and group A (RVA) and group C (RVC) are mostly affected. In Thailand, studies on RVA and PEDV have been reported periodically, whereas information on RVC is still limited. In this study, 769 samples (fecal and intestinal content) from pigs with diarrhea were collected from pig herds located in difference provinces throughout Thailand between 2011 and 2016. The specimens were tested using virus-specific RT-PCR to detect the gene encoding capsid protein VP7 and VP4 for RVC, complete 11 gene segments of RVA and S, ORF3, N genes for PEDV. Sequencing analyses showed that 6.6% (51/769) of samples were positive for RVC, 9.5% (73/769) for RVA and 19.9% (153/769) for PEDV. Co-infections of RVA/RVC accounted for 21.6% (11/51) of samples and of PEDV/RVC for 7.8% (4/51) of samples, while only three samples (3/51) or 5.8% tested positive for all three viruses. RV was detected in piglets up to 8 weeks old, while PEDV was often demonstrated from newborn up to 4 weeks of age. Infection severities were not associated with seasonality, since the virus was detected throughout the year. From our study, the G9 and P[13] as the dominant genotypes for RVA, while predominant genotypes of RVC were G1 and P[5]. Furthermore, genome constellation of the Thai RVA strains showed the predominance of Wa-like genotype with significant of reassortment between the porcine and human RVA strains. Comparison of 95 PEDV-positive samples indicated those Thai strains had close genetic relationship and resembled to previously identified PEDV from Thailand and China. Interestingly, eight Thai PEDV strains possessed the amino acid deletions in the N protein. Our findings provide substaintial information in regional rotavirus and PEDV strains in field circulations and may assist inclusions of suitable strains for future vaccine development.
DOI: 10.14457/cu.the.2018.107
title: Key Success Factors Of Starbucks Coffee In South Korea

description: Food and beverage is one of the industries that have been contributing economic growth toward South Korea economy for a decades; however, coffee market due to a relatively high competition and fierceness, coffee war, between international brands and local brands. This paper is trying to find the key success factors of Starbucks through various dimensions in South Korea market. This study is in order to find competitive advantages of Starbucks internationally and domestically. Observation is also held to find an insight data. The outcome shows that Korean coffee market has strength in it character conditions. The coffee quality and coffee experience with Americanization, third place, reward and class, and familiarity are key success factor of Starbucks in South Korea.
DOI: 10.14457/cu.the.2018.108
title: Decolorization of Reactive Black 5 using Soil Bacterial Consortium under a Combined Dynamic and Static Condition

description: Decolorization of Reactive Black 5 (RB-5) dye by isolated soil bacterial consortium had been conducted in this research. This study aims to determine the effect of operating conditions and kinetics of RB-5 decolorization by single strains and the consortium. Bacterial strains capable of RB-5 decolorization were isolated and identified as Micrococcus sp., Janibacter sp. and Rhodococcus sp. The RB-5 synthetic wastewater was used in the experiments with five days of incubation time. The studied conditions include combinations of shaking (dynamic) and static period of incubation, bacterial concentration, initial pH, temperature, and initial dye concentration. The results showed that the combined dynamic and static condition was important to RB-5 decolorization by the isolated bacteria. The decolorization with highest efficiency for all cultures was obtained at pH 7 with 2-day shaking period continued with 3-day static period for single strains and 1-day continued with 4-day for the consortium. The highest efficiency was given by Janibacter sp. (94.8% at 100 mg/L), which exhibited a good performance up to pH 11. All the decolorizations of RB-5 by single strains followed the first-order kinetics (4/-5 concentrations of 200 to 1,000 mg/L) but this was not observed with the consortium. Even though the decolorization efficiency of the consortium (88%) was not as high as the standalone Janibacter sp., the shaking period could be reduced from 2 days to 1 day without losing high ability to decolorize at pH 11. This suggests that working with consortium could give a benefit of reducing the aeration period compared to working with single strain, which will be useful for wastewater treatment application.