cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6949310
title: Supplementary Material for: Sex Differences in the Prevalence of and Risk Factors for Nonvascular Cognitive Function in Rural, Low-Income Elderly in Tianjin, China

description: <b><i>Background:</i></b> At the global level, dementia is the leading cause of dependence and disability among the elderly. Although the preponderant prevalence in women has been identified, the sex differences in risk factors were unclear. We aimed to evaluate the sex differences in the prevalence of nonvascular cognitive impairment and the risk factors among the elderly in rural China screened with the Mini-Mental State Examination (MMSE). <b><i>Methods:</i></b> Between 2014 and 2015, a population-based cross-section study was conducted to collect basic information among the elderly aged 60 years and over. Those participants with the previous history of stroke or heart disease were excluded in this study. Nonvascular cognitive impairment was assessed using the MMSE scores. <b><i>Results:</i></b> The prevalence of cognitive impairment was 32.4% overall, 25.6% in men and 38.1% in women. In the multivariate analysis, older age and lower education were risk factors both in men and in women; older, large waist circumference was a protective factor for cognitive function in men; higher blood pressure was the risk factor in women. <b><i>Conclusion:</i></b> These findings suggest that it is crucial to manage and control hypertension and improve educational attainment in order to reduce the prevalence and burden of nonvascular cognitive impairment among low-income residents, both men and women, in rural China.
DOI: 10.6084/m9.figshare.6949310.v1
title: Supplementary Material for: Sex Differences in the Prevalence of and Risk Factors for Nonvascular Cognitive Function in Rural, Low-Income Elderly in Tianjin, China

description: <b><i>Background:</i></b> At the global level, dementia is the leading cause of dependence and disability among the elderly. Although the preponderant prevalence in women has been identified, the sex differences in risk factors were unclear. We aimed to evaluate the sex differences in the prevalence of nonvascular cognitive impairment and the risk factors among the elderly in rural China screened with the Mini-Mental State Examination (MMSE). <b><i>Methods:</i></b> Between 2014 and 2015, a population-based cross-section study was conducted to collect basic information among the elderly aged 60 years and over. Those participants with the previous history of stroke or heart disease were excluded in this study. Nonvascular cognitive impairment was assessed using the MMSE scores. <b><i>Results:</i></b> The prevalence of cognitive impairment was 32.4% overall, 25.6% in men and 38.1% in women. In the multivariate analysis, older age and lower education were risk factors both in men and in women; older, large waist circumference was a protective factor for cognitive function in men; higher blood pressure was the risk factor in women. <b><i>Conclusion:</i></b> These findings suggest that it is crucial to manage and control hypertension and improve educational attainment in order to reduce the prevalence and burden of nonvascular cognitive impairment among low-income residents, both men and women, in rural China.
DOI: 10.6084/m9.figshare.6949316.v1
title: Supplementary Material for: Estimation of the Prevalence of Amyotrophic Lateral Sclerosis in the United States Using National Administrative Healthcare Data from 2002 to 2004 and Capture-Recapture Methodology

description: <b><i>Background:</i></b> National administrative healthcare data may be used as a case-finding method for prevalence studies of chronic disease in the United States, but the completeness of ascertainment likely varies depending on the disease under study. <b><i>Methods:</i></b> We used 3 case-finding sources (Medicare, Medicaid, and Veterans Administration data) to estimate the prevalence of amyotrophic lateral sclerosis (ALS) in the United States for 2002–2004, and applied the capture-recapture methodology to estimate the degree of under-ascertainment when relying solely on these sources for case identification. <b><i>Results:</i></b> Case-finding completeness was 76% overall and did not vary by race, but was lower for males (77%) than for females (88%), and lower for patients under age 65 (66%) than patients over age 65 (79%). The uncorrected ALS prevalence ratio was 2.8/100,000 in 2002, 3.3/100,000 in 2003, and 3.7/100,000 in 2004. After correcting for under-ascertainment, the annual prevalence increased by approximately 1 per 100,000 to 3.7/100,000 in 2002 (95% CI 3.66–3.80), 4.4/100,000 in 2003 (95% CI 4.34–4.50), and 4.8/100,000 in 2004 (95% CI 4.76–4.91). <b><i>Conclusions:</i></b> Federal healthcare claims databases ascertained are a very efficient method for identifying the majority of ALS-prevalent cases in the National ALS Registry, and may be enhanced by having patients self-register through the registry web portal.
DOI: 10.6084/m9.figshare.6949304
title: Supplementary Material for: Serum Vitamin D Concentrations and Cognitive Change Over 20 Years: The Atherosclerosis Risk in Communities Neurocognitive Study

description: <b><i>Background/Aims:</i></b> 25-hydroxyvitamin D (25[OH]D) concentrations have been associated with cognitive decline and incident dementia in elderly populations; however, these relationships are susceptible to reverse causation. Less is known about the association of midlife 25(OH)D with long-term cognitive decline. <b><i>Methods:</i></b> This was a prospective cohort study of 13,044 participants (mean age 57 years at baseline) in the Atherosclerosis Risk in Communities Study. 25(OH)D was measured from serum collected at baseline (1990–1992) using liquid chromatography tandem high-sensitivity mass spectrometry. Cognition was assessed using 3 neuropsychological tests at 3 time points, which were combined into a composite cognitive <i>Z</i>-score. Multivariable-adjusted linear mixed-effects models with random intercepts and slopes were used to estimate associations between 25(OH)D and cognitive change over 20 years. <b><i>Results:</i></b> Compared to persons with sufficient 25(OH)D (≥30 ng/mL), those with deficient (&lt; 20 ng/mL) and intermediate (20–&lt; 30 ng/mL) 25(OH)D concentrations had similar cognitive decline in composite cognitive <i>Z</i>-scores (deficient versus sufficient: –0.035 [95% CI –0.104 to 0.033] and intermediate versus sufficient: –0.029 [95% CI –0.080 to 0.023]). <b><i>Conclusions:</i></b> Lower concentrations of 25(OH)D measured in midlife were not significantly associated with more rapid cognitive decline over a 20-year follow-up period. The results of this prospective study are less susceptible to reverse causation than prior studies.
DOI: 10.6084/m9.figshare.6949304.v1
title: Supplementary Material for: Serum Vitamin D Concentrations and Cognitive Change Over 20 Years: The Atherosclerosis Risk in Communities Neurocognitive Study

description: <b><i>Background/Aims:</i></b> 25-hydroxyvitamin D (25[OH]D) concentrations have been associated with cognitive decline and incident dementia in elderly populations; however, these relationships are susceptible to reverse causation. Less is known about the association of midlife 25(OH)D with long-term cognitive decline. <b><i>Methods:</i></b> This was a prospective cohort study of 13,044 participants (mean age 57 years at baseline) in the Atherosclerosis Risk in Communities Study. 25(OH)D was measured from serum collected at baseline (1990–1992) using liquid chromatography tandem high-sensitivity mass spectrometry. Cognition was assessed using 3 neuropsychological tests at 3 time points, which were combined into a composite cognitive <i>Z</i>-score. Multivariable-adjusted linear mixed-effects models with random intercepts and slopes were used to estimate associations between 25(OH)D and cognitive change over 20 years. <b><i>Results:</i></b> Compared to persons with sufficient 25(OH)D (≥30 ng/mL), those with deficient (&lt; 20 ng/mL) and intermediate (20–&lt; 30 ng/mL) 25(OH)D concentrations had similar cognitive decline in composite cognitive <i>Z</i>-scores (deficient versus sufficient: –0.035 [95% CI –0.104 to 0.033] and intermediate versus sufficient: –0.029 [95% CI –0.080 to 0.023]). <b><i>Conclusions:</i></b> Lower concentrations of 25(OH)D measured in midlife were not significantly associated with more rapid cognitive decline over a 20-year follow-up period. The results of this prospective study are less susceptible to reverse causation than prior studies.
DOI: 10.6084/m9.figshare.6949298
title: Leaf trichomes affect caterpillar feeding in an instar-specific manner

description: Leaf trichomes play well-established roles in defense against insect herbivores, both as a physical barrier that impedes herbivore movement and by mediating chemical defenses. However, little work has examined how different trichome types influence herbivory by herbivores at different stages of development. We examined whether caterpillar instar and trichome type (glandular or non-glandular) affected the ability of the specialist herbivore caterpillar <i>Manduca sexta</i> to initiate feeding on 11 Solanaceous species exhibiting variation in the density and type of leaf trichomes. Our results suggest that non-glandular trichomes are far more effective than glandular trichomes in deterring the initiation of feeding by first- and second-instar caterpillars. Meanwhile, neither glandular nor non-glandular trichomes significantly affected the ability of third-instar caterpillars to commence feeding. These findings suggest that while non-glandular trichomes deter feeding initiation by early instar caterpillars, the contribution of both trichomes on later instars may depend on effects after feeding initiation.
DOI: 10.6084/m9.figshare.6949298.v1
title: Leaf trichomes affect caterpillar feeding in an instar-specific manner

description: Leaf trichomes play well-established roles in defense against insect herbivores, both as a physical barrier that impedes herbivore movement and by mediating chemical defenses. However, little work has examined how different trichome types influence herbivory by herbivores at different stages of development. We examined whether caterpillar instar and trichome type (glandular or non-glandular) affected the ability of the specialist herbivore caterpillar <i>Manduca sexta</i> to initiate feeding on 11 Solanaceous species exhibiting variation in the density and type of leaf trichomes. Our results suggest that non-glandular trichomes are far more effective than glandular trichomes in deterring the initiation of feeding by first- and second-instar caterpillars. Meanwhile, neither glandular nor non-glandular trichomes significantly affected the ability of third-instar caterpillars to commence feeding. These findings suggest that while non-glandular trichomes deter feeding initiation by early instar caterpillars, the contribution of both trichomes on later instars may depend on effects after feeding initiation.
DOI: 10.6084/m9.figshare.6949289
title: Barbastelle survey Ashridge Pictures

description: Pictures of radio tracking survey<br>
DOI: 10.6084/m9.figshare.6949289.v1
title: Barbastelle survey Ashridge Pictures

description: Pictures of radio tracking survey<br>
DOI: 10.6084/m9.figshare.6949286
title: Dataset for: Granulocyte-macrophage colony-stimulating factor (GM-CSF) improves mouse peripheral nerve regeneration following sciatic nerve crush

description: Peripheral nerve injuries severely impair patients’ quality of life since full recovery is seldom achieved. Upon axonal disruption, the distal nerve stump undergoes fragmentation, and myelin breaks down; the subsequent regeneration progression is dependent on cell debris removal. In addition to tissue clearance, macrophages release angiogenic and neurotrophic factors that contribute to axon growth. Based on the importance of macrophages for nerve regeneration, especially during the initial response to injury, we treated mice with granulocyte-macrophage colony-stimulating factor (GM-CSF) at various intervals after sciatic nerve crushing. Sciatic nerves were histologically analyzed at different time intervals after injury for the presence of macrophages and indicators of regeneration. Functional recovery was followed by an automated walking track test. We found that GM-CSF potentiated early axon growth, as indicated by the enhanced expression of growth-associated protein at 7 days postinjury. Inducible nitric oxide synthase expression increased at the beginning and at the end of the regenerative process, suggesting that nitric oxide is involved in axon growth and pruning. As expected, GM-CSF treatment stimulated macrophage infiltration, which increased at 7 and 14 days; however, it did not improve myelin clearance. Instead, GM-CSF stimulated early brain-derived neurotrophic factor (BDNF) production, which peaked at 7 days. Locomotor recovery pattern was not improved by GM-CSF treatment. The present results suggest that GM-CSF may have beneficial effects on early axonal regeneration.
DOI: 10.6084/m9.figshare.6949286.v1
title: Dataset for: Granulocyte-macrophage colony-stimulating factor (GM-CSF) improves mouse peripheral nerve regeneration following sciatic nerve crush

description: Peripheral nerve injuries severely impair patients’ quality of life since full recovery is seldom achieved. Upon axonal disruption, the distal nerve stump undergoes fragmentation, and myelin breaks down; the subsequent regeneration progression is dependent on cell debris removal. In addition to tissue clearance, macrophages release angiogenic and neurotrophic factors that contribute to axon growth. Based on the importance of macrophages for nerve regeneration, especially during the initial response to injury, we treated mice with granulocyte-macrophage colony-stimulating factor (GM-CSF) at various intervals after sciatic nerve crushing. Sciatic nerves were histologically analyzed at different time intervals after injury for the presence of macrophages and indicators of regeneration. Functional recovery was followed by an automated walking track test. We found that GM-CSF potentiated early axon growth, as indicated by the enhanced expression of growth-associated protein at 7 days postinjury. Inducible nitric oxide synthase expression increased at the beginning and at the end of the regenerative process, suggesting that nitric oxide is involved in axon growth and pruning. As expected, GM-CSF treatment stimulated macrophage infiltration, which increased at 7 and 14 days; however, it did not improve myelin clearance. Instead, GM-CSF stimulated early brain-derived neurotrophic factor (BDNF) production, which peaked at 7 days. Locomotor recovery pattern was not improved by GM-CSF treatment. The present results suggest that GM-CSF may have beneficial effects on early axonal regeneration.
DOI: 10.6084/m9.figshare.6949274
title: Supporting Online Material - DNA sequence differences are determinants of meiotic recombination outcome

description: Raw data and plasmid sequences accompanying Brown SD, Mpaulo SJ, Asogwa MN, Jezequel M, Whitby MC, Lorenz A (2019) DNA sequence differences are determinants of meiotic recombination outcome. <br>
DOI: 10.5281/zenodo.4006014
title: Test 1. Geo-electrically monitored milli-fluidic experiment

description: Test 1 for uploading data corresponding to: Geoelectrically monitored milli-fluidic tracer test. For more details please refer to the Metadata_file.txt
DOI: 10.5281/zenodo.4006013
title: Test 1. Geo-electrically monitored milli-fluidic experiment

description: Test 1 for uploading data corresponding to: Geoelectrically monitored milli-fluidic tracer test. For more details please refer to the Metadata_file.txt
DOI: 10.6084/m9.figshare.6949190
title: "Boom, headshot!"

description: Data set for the following article:<br>Bushman, B. J.(in press). “Boom, headshot!”: Violent first-person shooter (FPS) video games that reward headshots train individuals to aim for the head when shooting a realistic firearm. <i>Aggressive Behavior</i>.
DOI: 10.6084/m9.figshare.6949079
title: Supplementary Material for: <b><i>Xenopus</i></b>: An Undervalued Model Organism to Study and Model Human Genetic Disease

description: The function of normal and defective candidate genes for human genetic diseases, which are rapidly being identified in large numbers by human geneticists and the biomedical community at large, will be best studied in relevant and predictive model organisms that allow high-speed verification, analysis of underlying developmental, cellular and molecular mechanisms, and establishment of disease models to test therapeutic options. We describe and discuss the pros and cons of the frog <i>Xenopus</i>, which has been extensively used to uncover developmental mechanisms in the past, but which is being underutilized as a biomedical model. We argue that <i>Xenopus</i> complements the more commonly used mouse and zebrafish as a time- and cost-efficient animal model to study human disease alleles and mechanisms.
DOI: 10.6084/m9.figshare.6949088
title: Supplementary Material for: Utility of Horizontal Sections of Scalp Biopsies in Differentiating between Androgenetic Alopecia and Alopecia Areata

description: <b><i>Background:</i></b> Androgenetic alopecia (AGA) and alopecia areata (AA) are common causes of alopecia which can sometimes be difficult to differentiate clinically. Horizontal sections of scalp biopsies are used to study non-cicatricial alopecias due to the ability to perform both quantitative and morphometric analysis of hair follicles on them. <b><i>Methods:</i></b> It was a prospective, cross-sectional study conducted to assess the utility of horizontal sections to differentiate between the alopecias. Fifty-two cases were included: 20 cases of male AGA, 11 of female AGA and 21 cases of AA. After clinical examination and dermoscopy, a skin biopsy was taken and subjected to transverse sectioning. Histopathological assessment was done by two dermatopathologists blinded to clinical details. <b><i>Results:</i></b> Among the quantitative parameters, terminal:vellus hair ratio (3.08 in AGA and 1.83 in AA, <i>p</i> = 0.0091) and anagen:non-anagen hair ratio (9.25 in AGA and 3.56 in AA, <i>p</i> = 0.0021) were significantly lower in AA. In qualitative parameters, peribulbar inflammation was seen in 63% of AA cases (<i>p</i> = 0.0001). Pigment casts were seen in twice the number of AA (57%) than AGA (26%) cases. Broad avascular stelae and focal trichomalacia were seen in 9.5% of AA cases. <b><i>Conclusion:</i></b> Besides peribulbar inflammation, we found a lower anagen:non-anagen hair ratio and presence of pigment casts in transverse sections of scalp biopsies favouring AA over AGA.
DOI: 10.6084/m9.figshare.6949064
title: Supplementary Material for: Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant <b><i>Escherichia coli</i></b> from South India

description: <b><i>Objectives:</i></b> The aim of this study was to analyze the prevalence of the CTX-M, TEM, SHV, VIM, NDM, and OXA genes in carbapenemase-producing <i>Escherichia coli</i> and their transmissibility at a tertiary care hospital in south India. <b><i>Materials and Methods:</i></b> Twenty-one carbapenem-resistant <i>E. coli</i> (carbapenem-resistant Enterobacteriaceae; CRE) were collected from the Sri Sathya Sai Institute of Higher Medical Sciences (Puttaparthi India). Resistance to antibiotics was analyzed by Vitek-2, and the identity of the isolates was confirmed by 16S rDNA sequencing. RAPD and enterobacterial repetitive intergenic consensus (ERIC)-PCR were performed for molecular typing. Metallo-β-lactamase production was confirmed by a double disc synergy test. The presence of the extended-spectrum β-lactamases CTX-M, TEM, and SHV and of the carbapenemases NDM, VIM, and OXA was determined by PCR. Carbapenemase variants were further confirmed by sequencing. The transmissibility of the genes was tested by conjugation. <b><i>Results:</i></b> Twelve of the 21 (57%) carbapenem-resistant <i>E. coli</i> isolates were community acquired, indicating the spread of CRE in environmental samples. TEM and NDM-5 were found to be the major β-lactamases produced by the pathogens. OXA-181 was found in 5 of the isolates. All 21 isolates were found to harbor more than one of the tested β-lactamases, and all of the isolates were found to have the capacity to participate in conjugation; 15 of the transconjugants were found to have acquired the tested β-lactamases, substantiating their ability to be transferred to other strains of bacteria. <b><i>Conclusion:</i></b> Monitoring of community-acquired carbapenem-resistant bacteria is very important as the association of resistance determinants with mobile genetic elements would present a serious clinical challenge.
DOI: 10.6084/m9.figshare.6948977.v1
title: Rare and phylogenetically distinct plant species exhibit less diverse root-associated pathogen communities

description: Raw ITS sequences for 529 root tip samples collected from 45 plant species in a subtropical forest dynamic plot, southern China
DOI: 10.6084/m9.figshare.6948965
title: RGE.xlsx

description: this is relative gene expression level of myb28 over expressed and wild type
DOI: 10.6084/m9.figshare.6948956
title: PONE-D-18-14488_Newborn careseeking.dta

description: Endline Household Survey of a paired cluster-randomised controlled trial (cRCT) which was conducted in 14 rural sub-districts of Bangladesh.
DOI: 10.6084/m9.figshare.6948965.v1
title: RGE.xlsx

description: this is relative gene expression level of myb28 over expressed and wild type
DOI: 10.6084/m9.figshare.6948929
title: GLs .xlsx

description: Glucosinolate content was analyzed using 4 weeks old plants. Five plants of each line were used as biological replicates. Plants were cut from top of the soil, put in liquid nitrogen to stop chemical reactions and transferred to freeze-drier for 3 days. Freeze-dried samples were finely ground using micro-dismemberator U (B. Braun Biotech International). Finely grounded material was sent to NIOO for dedicated glucosinolate analysis. Briefly, samples were boiled in 70% MeOH followed by washing two times in 1 ml of 70% MeOH and two times with 1 mL MQ water and one times in 2mL of NaOAC 16 hr ahead of treatment with 20 μl arylsulfatase. Following arylsulfatase treatment freeze dried elute was redissolved in 1mL MQ water and filtered over a 20 μm filter. Separation and detection of glucosinolates were performed using reversed phase C-18 column and photodiode array detector respectively. Glucosinolates measurement and concentration calculation was done following the procedure of Kabouw et al., (2010). A list of measured glucosinolates and their abbreviated names are given in
DOI: 10.6084/m9.figshare.6948956.v1
title: PONE-D-18-14488_Newborn careseeking.dta

description: Endline Household Survey of a paired cluster-randomised controlled trial (cRCT) which was conducted in 14 rural sub-districts of Bangladesh.
DOI: 10.6084/m9.figshare.6948929.v1
title: GLs .xlsx

description: Glucosinolate content was analyzed using 4 weeks old plants. Five plants of each line were used as biological replicates. Plants were cut from top of the soil, put in liquid nitrogen to stop chemical reactions and transferred to freeze-drier for 3 days. Freeze-dried samples were finely ground using micro-dismemberator U (B. Braun Biotech International). Finely grounded material was sent to NIOO for dedicated glucosinolate analysis. Briefly, samples were boiled in 70% MeOH followed by washing two times in 1 ml of 70% MeOH and two times with 1 mL MQ water and one times in 2mL of NaOAC 16 hr ahead of treatment with 20 μl arylsulfatase. Following arylsulfatase treatment freeze dried elute was redissolved in 1mL MQ water and filtered over a 20 μm filter. Separation and detection of glucosinolates were performed using reversed phase C-18 column and photodiode array detector respectively. Glucosinolates measurement and concentration calculation was done following the procedure of Kabouw et al., (2010). A list of measured glucosinolates and their abbreviated names are given in