cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25549/examiner-c44-82274
title: Ned Redding family home at 8803 South Wilton Place, 1954

description: 6 images. Ned Redding family home at 8803 South Wilton Place, 7 April 1954. Ned Redding -- 48 years (candidate for Congress in 26th district); Audrey Redding (wife); Marion Redding (daughter) -- 19 years; Ned R. Redding -- 17 years (son).; Caption slip reads: "Photographer: Miller. Date: 1954-04-07. Reporter: Scott. Assignment: Ned Redding family home -- 8803 South Wilson Place. Ned Redding, 48, Democratic candidate for Congress in 26th district. Wife, Audrey Redding; Daughter, Marion, 19; Son, Ned R. Redding, 17. Family layout Monday page".; Supplementary material reads: "#46: Ned Redding drives long shot, as he and son, Ned, 17, (both golf enthusiasts) and daughter, Marion, 19, watch admiringly. #45: Ned Redding and his daughter, Marion, 19, check over music at piano where two of them sometimes play duets. #34/42: Ned, 17, winds up to pitch a fast one to father on neighborhood playground. Father and son have been taking in baseball games regularly together for years. #37: Ned, 17, family "comic," mimics a bit of political oratory in family den to amusement of rest of family. #34/42: Ned, 17, winds up to pitch a fast one to father on neighborhood playground. Father and son have been taking in baseball games regularly together for years. #37: Ned, 17, family "comic," mimics a bit of political oratory in family den to amusement of rest of family. #16/19: Ned Redding plants affectionate kiss on cheek of wife, Audrey, under rose arbor in their garden. #51: Ned Redding takes stroll with son, Ned, 17, and daughter, Marion, 19. #33: Ned Redding shows daughter, Marion, 19, some of the fine points of golf".; Supplementary material continues: #77: In family den, Marion leafs through ballet programs as her mother, who taught ballet for years, strikes a ballet pose. (Marion also studied ballet and the pair go to many programs together. #26?: Ned Redding and wife, Audrey, say goodbye to Ned, 17, and Marion, 19, at front door as they leave to work together on their publications. #18: Family conference on one of the Redding community newspapers. #21/22: Everybody helps in the Redding family -- including cooking chores. #42/7: Ned Redding and wife Audrey try not to 'bring work home with them,' but in emergency take over children's homework desk in hallway cove. #42: Redding family gather for music session as Ned, 17, and Marion, 19, choose their records to play on den phonograph. #38: Measuring up, a regular occurence in Redding household. Marion, 19, checks father's 5'8 1/2" inches against brother Ned's 6'1".
DOI: 10.25549/examiner-c44-52399
title: Dodgers arrival, International Airport, 1959

description: 13 images. Dodgers arrival, International Airport, 9 October 1959. Crowd shots; Duke Snider; Larry Sherry; Rosalind Wyman; Chuck Essegian; Walter Alston; Mr. and Mrs. Walter O'Malley.; Caption slip reads: "Photographer: Snow. Date: 10-09. Assignment: Dodgers arrive. 140-109: Walter Alston greets crowd. 94-93, L to R: Duke Snider and Larry Sherry sign autographs. 62-61: Larry Sherry greets crowd".
DOI: 10.25549/examiner-c44-44106
title: Desert circus (Palm Springs), 1952

description: 16 images. Desert circus (Palm Springs), 6 April 1952. Adrian Booth; David Brian; Marilyn Meyer -- 17 years (queen); Frank Bogert; Jack Klohr.
DOI: 10.25549/examiner-c44-51822
title: Dodgers welcome and parade, 1958

description: 41 images. Dodgers welcome and parade, 18 April 1958. Charles Dressen; Walter Alston; Mayor and Mrs. George Christopher; George Hearst; Walter O'Malley; Mayor Norris Poulson; Norris Brandt (Poulson's grandson); F.M. Parker; Danny Parker -- 3 1/2 years; Bobby Parker -- 4 1/2 years. (Negatives by Paegel, Olmo, Snow, Mitchell).; Caption slip reads: "Photographer: Mitchell. Date: 1958-04-18. Reporter: Bernstein. Assignment: Baseball at City Hall. 7: Charles Dressen; Walter Alston. 8: Parade looking north from First Street and Beaudry. 47: Mayor George Christopher and his wife from San Francisco. 48. Mayor George Christopher; Mrs. Christopher; George Hearst. 9: Walter O'Malley wears Serape and sombrero, gift of Olvera Street. 10: Walter O'Malley with (monkey) Gino. 57: Mayor Norris Poulson; Walter O'Malley; Home plate. 58: L to R: Mayor Norris Poulson; grandson Norris Brandt; Mrs. Poulson. 59: Walter O'Malley; Foghorn Murphy on horse patches. 60: Mayor Norris Poulson".; Other caption slip reads: "Photographer: Paegel. Date: 1958-04-18. Reporter: Thackrey. Assignment: Crowd sidebar. F.M. Parker of Alhambra and his grandsons, Danny, 3 1/2, who caught a ball, and Bobby, 4 1/2, who didn't".
DOI: 10.25549/examiner-c44-52360
title: Side bar of Coliseum (Dodgers baseball), 1959

description: 9 images. Side bar of Coliseum (Dodgers baseball), 3 October 1959. Sam Sansone; Hal Jensen; Ernest Lisi; Jerry Buttice; Ray Martin -- 12 years; Jim Espositio; Lenny Hahn -- 13 years; Jimmy White -- 12 years; Steve Weber -- 11 years; Robin Day -- 11 years; Bill Stunkard -- 13 years; Mr. and Mrs. Jacob Olshansky; Dan Olshansky -- 6 years; Ellen Olshansky -- 9 years; Tom Arthur. (Negatives of traffic signals to be used at the game).; Caption slip reads: "Photographer: Miller. Date: 1959-10-03. Reporter: Donoghue. Assignment: Concessions, Coliseum. Sunday. 35-36: Tom Arthur, concession manager at Coliseum, with kingsize hotdog, the like of which will sell by the tens of thousands at opening of World Series home game. 121: Dodgers camera stars test new equipment for first home World Series game -- from left: Chief Photographer Sam Sansone and prize-winning lensman Hal Jensen. 50: Traffic signs all lined up for what is expected to be the granddaddy of all traffic treks to giant oval. 49: Western Union technicians get some of the 56 press teletypes at Coliseum in order for Sunday's classic -- from left: Ernest Lisi, Pacoima; and Jerry Buttice. 105: Ray Martin, 12, Ascension Lutheran Parochial School, is champ autograph collector. Walt Alston okayed spot for him at clubhouse door. 106: Dodgers Groundkeeper Jim Espositio. 122: Junior attendance champs, who saw total of 107 games at Coliseum this year: Lenny Hahn, 13; Jimmy White, 12; Steve Weber, 11. Bats are gifts of Don Demeter".
DOI: 10.25549/examiner-c44-81845
title: County sponsored pet show (Rosewood Park, 5600 East Harbor Street, East Los Angeles), 1952

description: 4 images. County sponsored pet show (Rosewood Park, 5600 East Harbor Street, East Los Angeles), 25 July 1952. Barbara Lynn Gold; Judy Schindewolf; Gary Priebe; Gloria Maese; Rosemary Dejuilio; Ann Marie Kantz; Dianne Dominguez.; Caption slip reads: "Photographer: Snow. Assignment: For Dave Brandman. 82: Barbara Lynn Gold; 'Beulah'; 'Candy'; 'Johnny Ray'. 81: Crowd. 31: Judy Schindewolf (Helly Pelly). 32: Gary Priebe ('Daisey'). 29: Gloria Maese (Coco). #30: Rosemary DeJuilio ('Tiny'). 7: Anne Marie Kantz; Dianne Dominguez".; Supplementary material reads: "Memo from Dave Brandman. July 23. City Desk: Please assign a photographer to be at Rosewood Park (5600 E. Harbor St., East L.A.) at 11 a.m. Friday, 7/25, to get some shots of a County sponsored Pet Show being held there".
DOI: 10.25549/examiner-c44-50666
title: Dillingham wedding, 1958

description: 3 images. Dillingham wedding, 14 February 1958. Walter Dillingham; Elizabeth Carr. (Contacts and negatives to Carter Ludlow). (Sleeve reads S-12310).; Caption slip reads: "Photographer: Wilson. Date: 02-14. Assignment: Dillingham wedding. 58/03/04: Walter Dillingham and Elizabeth Carr".; Supplemental material reads: "Photo assignment for Friday February 14 at 4:30 p.m. Please go to home of Betty Carr, 301 Via Nicolas, Pacific Palisades (Sunset Boulevard almost to Coast Highway, turn right on 17300 block at Los Liones Drive, right on Via Nicolas) GL 4-1703, get shots of wedding of Walter Dillingham and Betty Carr. Candid, Close together, for two col cut. Bride and groom only. Couple of set ups, animated, unaware of camera. Contacts Monday. Thanks, Carter Ludlow".
DOI: 10.25549/examiner-c44-44718
title: Physical Education conference at San Fernando Valley... square dancing... groups and individuals, 1952

description: 17 images. Physical Education conference at San Fernando Valley... square dancing... groups and individuals, 7 April 1952. Darrel Browne, caller; Darrin Tollin, child; John Scribner, child, partners.
DOI: 10.25549/examiner-c44-79495
title: Christmas parking tickets in San Fernando, 1954

description: 2 images. Christmas parking tickets in San Fernando, 22 December 1954. Officer George E. Jones; Mrs. Linda Taccona (accepting Christmas greeting parking ticket).; Caption slip reads: “Photographer: Glickman. Date: 1954-12-22. Assignment: Xmas parking tickets San Fernando. 2 shots of Officer George E. Jones as he hands Xmas greeting parking ticket to Xmas shopper, Mrs. Linda Taccona in San Fernando”.
DOI: 10.25549/examiner-c44-79508
title: Narcotic shakedown plunged against power pole, 1954

description: 3 images. Narcotic shakedown plunged against power pole, 15 December 1954. Bernabe Sena (suspect); Fredrico Munoz (suspect); Officer Jack Smith (investigating).; Supplementary material reads: “Gershon. City desk. Car used by four former felons to evade routine Narc. shakedown is shown after it plunged against power pole. Vice Squad Officer Werth Harvey searching car as above. He sought narcs. L to R: Bernabe Sena, 31, 915 Averill Avenue, Fredrico Munoz, 21, 445 West 13th street, and Vice Squad Officer Jack Smith. Latter is searching Munoz’ clothing for possible narc hideaway”.
DOI: 10.25549/examiner-c44-79517
title: Gallstones (Cedars of Lebanon Hospital), 1954

description: 4 images. Gallstones (Cedars of Lebanon Hosp), 15 December 1954. Mrs. Walter Small; Dulcie Small- 20 yrs. (both had Gallstones operation).; Caption slip reads: “Photographer: Mack. Date: 1954-12-15. Reporter: Clausen. Assignment: Gallstone kids. 19/20/35/36: Mrs. Walter Small, right, and daughter, Dulcie, 20, in Cedars of Lebanon Hospital where they both had gallstones removed”.
DOI: 10.25549/examiner-c44-79691
title: Sex hearing (teacher), 1959

description: 2 images. Sex hearing (teacher), 4 August 1959. Mrs. Carol Cook; Cecil Cook (teacher).; Caption slip reads: "Photographer: Miller. Reporter: Slation. Assignment: Sex hearing. 221/222: Mrs. Carol Cook. 17/18: Cecil Cook.
DOI: 10.5281/zenodo.10982175
title: ELITE SLWR: global 1km 5-min SLUR at SIN Grid-Terra (2011_350-365)

description: The Essential thermal Infrared remoTe sEnsing (ELITE) product suite currently has four types of products, including land surface temperature (LST: clear-sky and all-sky), emissivity (NBE: narrowband emissivity; BBE: broadband emissivity; and spectral emissivity), the component of surface radiation and energy budget (SLUR: surface longwave upwelling radiation; SLDR: surface longwave downward radiation SLDR; SLNR: surface longwave net radiation), and the component of Earth’s radiation budget (OLR; outgoing longwave radiation; RSR: reflected solar radiation). The spatial-temporal resolutions of the ELITE products are mainly determined by the employed satellite data sources. For more information about ELITE products, please refer to the website (https://elite.bnu.edu.cn).

This dataset is ELITE surface longwave upward radiation (SLUR) product at 1km spatial resolution and 5-min temporal resolution. It is provided on a Sinusoidal grid and ranges from 2000 to 2022 (continuously updated). This dataset was generated from the Moderate Resolution Imaging Spectrometer (MODIS) data using the algorithms developed by Cheng et al. (2016).

This is the ELITE SLUR product in 2011 (DOY: 350-365). Please click here to download the ELITE SLUR product in 2012 (DOY: 001-016).

Dataset Characteristics:



Spatial Coverage: Global

Temporal Coverage: 2011-12-16 ~ 2011-12-31

Spatial Resolution: 1 km

Temporal Resolution: 5-min

Data Format: h5

Projection: Sinusoidal

Scale: 0.05

Offset: -1000


Citation(Please cite these papers when using the data):



Cheng, J., & Liang, S. (2016). Global estimates for high spatial resolution clear-sky land surface upwelling longwave radiation from MODIS data. IEEE Transactions on Geoscience and Remote Sensing, 54, 4115-4129.


If you have any questions, please contact Prof. Jie Cheng (eliteqrs@126.com).
DOI: 10.5281/zenodo.10982176
title: ELITE SLWR: global 1km 5-min SLUR at SIN Grid-Terra (2011_350-365)

description: The Essential thermal Infrared remoTe sEnsing (ELITE) product suite currently has four types of products, including land surface temperature (LST: clear-sky and all-sky), emissivity (NBE: narrowband emissivity; BBE: broadband emissivity; and spectral emissivity), the component of surface radiation and energy budget (SLUR: surface longwave upwelling radiation; SLDR: surface longwave downward radiation SLDR; SLNR: surface longwave net radiation), and the component of Earth’s radiation budget (OLR; outgoing longwave radiation; RSR: reflected solar radiation). The spatial-temporal resolutions of the ELITE products are mainly determined by the employed satellite data sources. For more information about ELITE products, please refer to the website (https://elite.bnu.edu.cn).

This dataset is ELITE surface longwave upward radiation (SLUR) product at 1km spatial resolution and 5-min temporal resolution. It is provided on a Sinusoidal grid and ranges from 2000 to 2022 (continuously updated). This dataset was generated from the Moderate Resolution Imaging Spectrometer (MODIS) data using the algorithms developed by Cheng et al. (2016).

This is the ELITE SLUR product in 2011 (DOY: 350-365). Please click here to download the ELITE SLUR product in 2012 (DOY: 001-016).

Dataset Characteristics:



Spatial Coverage: Global

Temporal Coverage: 2011-12-16 ~ 2011-12-31

Spatial Resolution: 1 km

Temporal Resolution: 5-min

Data Format: h5

Projection: Sinusoidal

Scale: 0.05

Offset: -1000


Citation(Please cite these papers when using the data):



Cheng, J., & Liang, S. (2016). Global estimates for high spatial resolution clear-sky land surface upwelling longwave radiation from MODIS data. IEEE Transactions on Geoscience and Remote Sensing, 54, 4115-4129.


If you have any questions, please contact Prof. Jie Cheng (eliteqrs@126.com).
DOI: 10.25549/examiner-c44-52356
title: Side bar of Coliseum (Dodgers baseball), 1959

description: 9 images. Side bar of Coliseum (Dodgers baseball), 3 October 1959. Sam Sansone; Hal Jensen; Ernest Lisi; Jerry Buttice; Ray Martin -- 12 years; Jim Espositio; Lenny Hahn -- 13 years; Jimmy White -- 12 years; Steve Weber -- 11 years; Robin Day -- 11 years; Bill Stunkard -- 13 years; Mr. and Mrs. Jacob Olshansky; Dan Olshansky -- 6 years; Ellen Olshansky -- 9 years; Tom Arthur. (Negatives of traffic signals to be used at the game).; Caption slip reads: "Photographer: Miller. Date: 1959-10-03. Reporter: Donoghue. Assignment: Concessions, Coliseum. Sunday. 35-36: Tom Arthur, concession manager at Coliseum, with kingsize hotdog, the like of which will sell by the tens of thousands at opening of World Series home game. 121: Dodgers camera stars test new equipment for first home World Series game -- from left: Chief Photographer Sam Sansone and prize-winning lensman Hal Jensen. 50: Traffic signs all lined up for what is expected to be the granddaddy of all traffic treks to giant oval. 49: Western Union technicians get some of the 56 press teletypes at Coliseum in order for Sunday's classic -- from left: Ernest Lisi, Pacoima; and Jerry Buttice. 105: Ray Martin, 12, Ascension Lutheran Parochial School, is champ autograph collector. Walt Alston okayed spot for him at clubhouse door. 106: Dodgers Groundkeeper Jim Espositio. 122: Junior attendance champs, who saw total of 107 games at Coliseum this year: Lenny Hahn, 13; Jimmy White, 12; Steve Weber, 11. Bats are gifts of Don Demeter".
DOI: 10.25549/examiner-c44-82633
title: Divorce, 1954

description: 2 images. Divorce, 15 April 1954. Mrs. Marjorie Grossel (wife of Jeff Chandler).; Caption slip reads: "Photographer: Brunk. Date: 1954-04-15. Reporter: Simmons. Assignment: Divorce on Cruelty, Dept. 53. b 25/26: Mrs. Marjorie Grossel, wife of Jeff Chandler".
DOI: 10.25549/examiner-c44-81445
title: Zeta Tau Alpha Sorority Convention, 1952

description: 4 images. Zeta Tau Alpha Sorority Convention, 6 July 1952. Mrs. Jack Stanley; Mrs. Ray Crawford; Mrs. Rodney R. Garner; Mrs. Sherman A. Todd. (Sleeve reads: S-9680).; Caption slip reads: "Photographer: Monte Verde. Assignment: Zeta Tau Alpha Sorority Convention. #80, 81, 31, 32, L to R: Mrs. Jack Stanley; Mrs. Ray Crawford; Mrs. Rodney R. Garner; Mrs. Sherman A. Todd".; Supplementary material reads: "Ward club photo. June 6. Friday noon, Huntington Hotel in Pasadena. Zeta Tau Alpha sorority, group will meet photog. at desk in front lobby. Picture is for June 15 release with story of sorority's annual convention at Huntington June 23".
DOI: 10.25549/examiner-c44-81974
title: Beach Club, Santa Monica, 1952

description: 14 images. Beach Club, Santa Monica, 24 May 1952. Mrs. Clifford Lord; Virginia Haley; Mrs. John Goggin; Mrs. Woodward Taylor, Junior; Mrs. Henry Thompson; Erin Considine; Mrs. Van Buren Jarvis; Mrs. Robert Bundy, Junior; Mrs. Ralph Young, Junior; Bill Davidson; Mrs. Robert Jewell; Mrs. Guy Cochran; Mrs. Robert Angell. (Sleeve reads: S-9680).; Caption slip reads: "Photographer: Miller. Date: 1952-05-24. Assignment: Beach Club, Santa Monica. 37/38: Mrs. Clifford W. Lord; Virginia Haley; Mrs. John Goggin; Mrs. Woodward Taylor, Jr. 9/10/43: Mrs. Henry Thompson; Erin Considine; Mrs. Van Buren Jarvis. 44/21/22: Mrs. Van Buren Jarvis; Mrs. Henry Thompson; Erin Considine. 11/12: Mrs. Robert Bundy, Jr.; Mrs. Ralph Young, Jr.; Mrs. Robert Jewell; Mrs. Guy Cochran; Mrs. Robert Angell. 31/32: Mrs. Robert Bundy, Jr.; Mrs. Ralph Young, Jr.; Mrs. Robert Jewell. 23/24: Bill Davidson; Virginia Haley; Mrs. Clifford W. Lord; Mrs. John Goggin; Mrs. Woodward Taylor, Jr. 45/46: Virginia Haley; Mrs. Clifford W. Lord; Mrs. John Goggin; Mrs. Woodward Taylor, Jr."
DOI: 10.25549/examiner-c44-81955
title: Beverly Hills burglar, 1952

description: 8 images. Beverly Hills burglar, 3 August 1952. Detective Sergeant Ray Borders; Harvey Allen (suspect); Sergeant F.H. Coldford.
DOI: 10.57745/mnn7cs
title: datasociality.tab

description: :unav
DOI: 10.6084/m9.figshare.25816495
title: Supplementary Material for: The role of PD-L1 expression in pituitary tumours: lessons from the current literature

description: Background: Programmed cell death-1 (PD-1) and PD ligand-1 (PD-L1) expression predicts the biological behaviour, aggres-siveness and response to immune checkpoint inhibitors in different cancers. We reviewed the published data on PD-L1 ex-pression in pituitary tumours, from the perspective of its biological role and prognostic usefulness.
Summary: A literature review focused on PD-L1 expression in pituitary tumours was performed. Six immunohistochemistry-based studies which assessed PD-L1 positivity in pituitary tumours were included, encompassing 704 patients. The cohort consisted of 384 (54.5%) non-functioning tumours and 320 (43.5%) functioning pituitary tumours. PD-L1 expression was posi-tive in 248 cases (35.2%). PD-L1 positivity rate was higher in functioning than in non-functioning tumours (46.3% vs 26.0%; p&lt;0.001), but also higher in growth hormone-secreting tumours (56.7%) and prolactinomas (53.6%) than in thyrotroph (33.3%) or corticotroph tumours (20.6%). While proliferative pituitary tumours showed higher rate of PD-L1 positivity than non-proliferative tumours (p&lt;0.001), no association with invasion or recurrence was found. 
Key messages: PD-L1 is expressed in a substantial number of pituitary tumours, predominantly in the functioning ones. PD-L1 positivity rates were significantly higher in proliferative pituitary tumours in comparison to non-proliferative tumours, but no differences were found concerning invasive or recurrent pituitary tumours. More studies following homogeneous and standardised methodologies are needed to fully elucidate the role and usefulness of PD-L1 expression in pituitary tumours.
DOI: 10.25549/examiner-c44-79404
title: Parrish divorce, 1954

description: 2 images. Parrish divorce, 1 September 1954. Attorney Max Gilford; Muzzy Marcelino.; Caption slip reads: “Photographer: Rustan. Date: 1954-09-01. Assignment: Parrish divorce. 67: Atty. Max Gilford; Muzzy Marcelino. 68: Muzzy Marcelino”.
DOI: 10.25549/examiner-c44-44488
title: Divorce, 1952

description: 3 images. Divorce, 8 April 1952. Gloria Ross -- 25 years.
DOI: 10.25549/examiner-c44-44459
title: Hit -- run suspect ...Georgia Street Receiving Hospital, 1952

description: 2 images. Hit -- run suspect ...Georgia Street Receiving Hospital, 6 April 1952. Aron Junior -- 22 years (Professional boxer of 1716 14th Street, Santa Monica following accident in which he was booked on felony manslaughter charge. He left scene of accident... woman in car in which he collided with died from injuries).
DOI: 10.25549/examiner-c44-50897
title: Orange show princesses, 1958

description: 3 images. Orange show princesses, 15 March 1958. Linda Belanger -- 17 years; Doris Rau -- 17 years; Joanne Cobb -- 19 years; Anne Davis -- 17 years; Anne Davis -- 17 years; Linda Cowie -- 19 years.; Caption slip reads: "Photographer: Rustan. Date: 1958-03-15. Reporter: Waymire. Assignment: Orange show princesses. 47/48/65: Left to right: Princess (one of whom will be queen) appeal to 'Citrus Sam', the midget, for the queen's crown. From left: Linda Belanger, 17: Doris Rau, 17; Joanne Cobb, 19; Anne Davis, 17; Linda Cowie, 19".
DOI: 10.25549/examiner-c44-81969
title: Beach Club, Santa Monica, 1952

description: 14 images. Beach Club, Santa Monica, 24 May 1952. Mrs. Clifford Lord; Virginia Haley; Mrs. John Goggin; Mrs. Woodward Taylor, Junior; Mrs. Henry Thompson; Erin Considine; Mrs. Van Buren Jarvis; Mrs. Robert Bundy, Junior; Mrs. Ralph Young, Junior; Bill Davidson; Mrs. Robert Jewell; Mrs. Guy Cochran; Mrs. Robert Angell. (Sleeve reads: S-9680).; Caption slip reads: "Photographer: Miller. Date: 1952-05-24. Assignment: Beach Club, Santa Monica. 37/38: Mrs. Clifford W. Lord; Virginia Haley; Mrs. John Goggin; Mrs. Woodward Taylor, Jr. 9/10/43: Mrs. Henry Thompson; Erin Considine; Mrs. Van Buren Jarvis. 44/21/22: Mrs. Van Buren Jarvis; Mrs. Henry Thompson; Erin Considine. 11/12: Mrs. Robert Bundy, Jr.; Mrs. Ralph Young, Jr.; Mrs. Robert Jewell; Mrs. Guy Cochran; Mrs. Robert Angell. 31/32: Mrs. Robert Bundy, Jr.; Mrs. Ralph Young, Jr.; Mrs. Robert Jewell. 23/24: Bill Davidson; Virginia Haley; Mrs. Clifford W. Lord; Mrs. John Goggin; Mrs. Woodward Taylor, Jr. 45/46: Virginia Haley; Mrs. Clifford W. Lord; Mrs. John Goggin; Mrs. Woodward Taylor, Jr."
DOI: 10.6084/m9.figshare.25816486
title: Supplementary Material for: Murine Ribonuclease 6 Limits Bacterial Dissemination During Experimental Urinary Tract Infection

description: Introduction: The Ribonuclease (RNase) A superfamily encodes cationic antimicrobial proteins with potent microbicidal activity toward uropathogenic bacteria. Ribonuclease 6 (RNase6) is an evolutionarily conserved, leukocyte-derived antimicrobial peptide with potent microbicidal activity toward uropathogenic Escherichia coli (UPEC), the most common cause of bacterial urinary tract infections (UTI). In this study, we generated Rnase6 deficient mice to investigate the hypothesis that endogenous RNase 6 limits host susceptibility to UTI. 

Methods: We generated a Rnase6EGFP knock-in allele to identify cellular sources of Rnase6 and determine the consequences of homozygous Rnase6 deletion on antimicrobial activity and UTI susceptibility. 

Results: We identified monocytes and macrophages as the primary cellular sources of Rnase6 in bladders and kidneys of Rnase6EGFP/+ mice. Rnase6 deficiency (i.e., Rnase6EGFP/EGFP) resulted in increased upper urinary tract UPEC burden during experimental UTI, compared to Rnase6+/+ controls. UPEC displayed increased intracellular survival in Rnase6 deficient macrophages. 

Conclusion: Our findings establish that RNase6 prevents pyelonephritis by promoting intracellular UPEC killing in monocytes and macrophages and reinforce the overarching contributions of endogenous antimicrobial RNase A proteins to host UTI defense.