cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/vwkbf-bb664
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-09-25</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.T</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-129/rawdata/</p>Cb_vero_201709250129.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-129/rawdata/Cb_vero_201709250129.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_201709250129_part61_7.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-129/3dimage_88633/Cb_vero_201709250129_part61_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a3rh2z
title: Occurrence Download

description: A dataset containing 281 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry MULTIPOLYGON(((-74.8602 4.33541,-74.86816 4.3265,-74.87403 4.31603,-74.87739 4.30491,-74.87833 4.29412,-74.8769 4.28239,-74.87297 4.27125,-74.86686 4.26138,-74.85864 4.25292,-74.84862 4.24636,-74.83779 4.24226,-74.8226 4.24056,-74.80763 4.24317,-74.7946 4.23527,-74.77885 4.23122,-74.75994 4.23267,-74.7432 4.24024,-74.73118 4.24984,-74.7225 4.2615,-74.71579 4.27826,-74.70259 4.30216,-74.69858 4.31798,-74.68868 4.32212,-74.67319 4.32138,-74.65834 4.325,-74.63405 4.33872,-74.62258 4.34886,-74.60963 4.36735,-74.59536 4.40282,-74.58497 4.40706,-74.57606 4.41289,-74.56809 4.42057,-74.56146 4.43011,-74.5547 4.44617,-74.55302 4.46338,-74.53517 4.47096,-74.5214 4.47176,-74.50887 4.47573,-74.49689 4.48323,-74.48795 4.49263,-74.48069 4.50591,-74.4774 4.52041,-74.46897 4.52864,-74.46228 4.54189,-74.46023 4.5566,-74.46455 4.57529,-74.47606 4.5956,-74.48616 4.60697,-74.49945 4.61433,-74.51443 4.61685,-74.52938 4.61424,-74.55246 4.6058,-74.5754 4.59142,-74.59321 4.57726,-74.60448 4.57657,-74.61514 4.57364,-74.63593 4.59724,-74.65118 4.60882,-74.66801 4.61479,-74.70186 4.61942,-74.728 4.61396,-74.74655 4.60714,-74.76411 4.60366,-74.77816 4.59785,-74.7963 4.58672,-74.81862 4.57637,-74.82833 4.56779,-74.83565 4.5573,-74.84011 4.54615,-74.84205 4.53351,-74.84117 4.52172,-74.83722 4.50914,-74.8306 4.4982,-74.82131 4.48888,-74.81147 4.48274,-74.80133 4.47904,-74.78987 4.47739,-74.77917 4.47804,-74.75689 4.4831,-74.73076 4.49768,-74.7079 4.5051,-74.70538 4.48655,-74.73292 4.46867,-74.74784 4.45595,-74.75794 4.45104,-74.78667 4.44173,-74.80373 4.43027,-74.82725 4.39982,-74.84015 4.38687,-74.84976 4.37396,-74.85538 4.36075,-74.8602 4.33541)))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Mammalia"
 ]
}

 The dataset includes 281 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002735-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/q88z6-9ny98
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-76/rawdata/</p>20150511_AK07_10076.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-76/rawdata/20150511_AK07_10076.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10076_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-76/3dimage_56508/20150511_AK07_10076_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0h7qf-r1883
title: perfusiondcrp_fixd_good track

description: MOh and Sahand
description: <strong>Tilt Series Date:</strong> 2015-08-24</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The sample was grown in perfusion chamber and fixed for 30 minutes with 4% formaldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1.5ul gold beads.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Lm is taken before and after fixing. In the LM after fixing the nanowires were shrunk. Although the cells looked good with formaldehyde we had lost long nanowires. 2 squares were imaged with correlation anyway.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-24-10/3dimage_60707/</p>perfusiondcrp_sq130002_part121_56.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-24-10/3dimage_60707/perfusiondcrp_sq130002_part121_56.rec"
        > <i class="download icon"></i> Download </a></p>

        perfusiondcrp_sq130002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-24-10/rawdata/perfusiondcrp_sq130002.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/znkc0-qmh33
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-09-25</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.T</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-128/rawdata/</p>Cb_vero_201709250128.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-128/rawdata/Cb_vero_201709250128.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_201709250128_part61_7.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-128/3dimage_88632/Cb_vero_201709250128_part61_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2y5en-g9g34
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-77/rawdata/</p>20150511_AK07_10077.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-77/rawdata/20150511_AK07_10077.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10077_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-77/3dimage_56510/20150511_AK07_10077_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mpe5v-mqt35
title: Dividing Caulobacter

description: Dividing Caulobacter, low-quality tomograms
description: <strong>Tilt Series Date:</strong> 2016-02-28</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -11.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500.</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 180/A2. defocus: -10um. magnification: 27500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-02-28-27/rawdata/</p>view80027.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-02-28-27/rawdata/view80027.mrc"
        > <i class="download icon"></i> Download </a></p>

        view80027_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-02-28-27/3dimage_73474/view80027_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.wxfazn
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ulmus glabra",
 "Country is Libya",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002739-250827131500795/datasets/export for details.
DOI: 10.22002/44tm2-36034
title: V. cholerae CK2188 delta-tcpR

description: Classical strain with ctxA deletionStrain name CK2188 (tcpR gene knonkout)Cell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2016-08-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Gabriela Kovacikova</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-06-13/rawdata/</p>20160806_YWC218_10013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-06-13/rawdata/20160806_YWC218_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160806_YWC218_10013_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-06-13/3dimage_76417/20160806_YWC218_10013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7v8zr7
title: Occurrence Download

description: A dataset containing 143 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ulmus glabra",
 "Country is Canada",
 "HasCoordinate is true"
 ]
}

 The dataset includes 143 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002738-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/hym0b-1mx17
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-08-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Take the sedimented cell clumps.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-40/3dimage_73158/</p>20150810_AK20_20040_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 14.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-40/3dimage_73158/20150810_AK20_20040_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150810_AK20_20040.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-40/rawdata/20150810_AK20_20040.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dvf3h-2k443
title: Lp02-fixed

description: WT Lp02 cells were fixed with GA and re-suspended in  PBS.
description: <strong>Tilt Series Date:</strong> 2017-11-21</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Shuman Lab</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-21-51/rawdata/</p>Legionella_fixed_20171121a0043.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-21-51/rawdata/Legionella_fixed_20171121a0043.mrc"
        > <i class="download icon"></i> Download </a></p>

        Legionella_fixed_20171121a0043_part61_8.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-21-51/3dimage_90496/Legionella_fixed_20171121a0043_part61_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.y4qc29
title: Occurrence Download

description: A dataset containing 17 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ulmus glabra",
 "Country is Armenia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 17 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002736-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/12t4z-m6r34
title: S. cerevisiae - 6210 + pAM21

description: Raw data files of S. cerevisiae - 6210 + pAM21
description: <strong>Tilt Series Date:</strong> 2016-09-24</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Babst Lab (Markus Babst and Akshay Moharir) - Provided strain with the plasmid transformed.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 6210 + pAM21(Vps36-V5-Strep in Ura plasmid). Grown to saturation overnight and cut back to 0.2 OD and grown until 0.6 OD. Media -> -URA YNB</p>
<strong>Sample Preparation:</strong> Pull down of P13 fraction using Dynabeads® M-280 Streptavidin from 5801D trial kit. B1 fraction (1st eluent of bound fraction - see Jensen Lab Protocols page 14).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-09-24-2/rawdata/</p>MVB1_NS.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.8 GB  
        
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        MVB1_NS_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 171.0 MB  
        
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        > <i class="download icon"></i> Download </a></p>