DOI: 10.5061/dryad.mj062
title: Data from: Weak sinusoidal electric fields entrain spontaneous Ca transients in the dendritic tufts of CA1 pyramidal cells in rat hippocampal slice preparations

description: Neurons might interact via electric fields and this notion has been
 referred to as ephaptic interaction. It has been shown that various types
 of ion channels are distributed along the dendrites and are capable of
 supporting generation of dendritic spikes. We hypothesized that generation
 of dendritic spikes play important roles in the ephaptic interactions
 either by amplifying the impact of electric fields or by providing current
 source to generate electric fields. To test if dendritic activities can be
 modulated by electric fields, we developed a method to monitor local
 Ca-transients in the dendrites of a neuronal population in acute rat
 hippocampal slices by applying spinning-disk confocal microscopy and
 multi-cell dye loading technique. In a condition in which the dendrites of
 CA1 pyramidal neurons show spontaneous Ca-transients due to added 50 μM
 4-aminopyridine to the bathing medium and adjusted extracellular potassium
 concentration, we examined the impact of sinusoidal electric fields on the
 Ca-transients. We have found that spontaneously occurring
 fast-Ca-transients in the tufts of the apical dendrites of CA1 pyramidal
 neurons can be blocked by applying 1 μM tetrodotoxin, and that the timing
 of the transients become entrained to sub-threshold 1-4 Hz electric fields
 with an intensity as weak as 0.84 mV/mm applied parallel to the
 somato-dendritic axis of the neurons. The extent of entrainment increases
 with intensity below 5 mV/mm, but does not increase further over the range
 of 5-20 mV/mm. These results suggest that population of pyramidal cells
 might be able to detect electric fields with biologically relevant
 intensity by modulating the timing of dendritic spikes.
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