cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22785495
title: Asteraceae of Serra da Confusão do Rio Preto, Quirinópolis, Goiás, Brazil

description: Abstract A floristic inventory and a taxonomic treatment of Asteraceae from Serra da Confusão do Rio Preto in the state of Goiás are provided. Collections were carried out from July 2017 to December 2018 in a fragment of cerrado sensu stricto with transition to cerrado rupestre (rocky cerrado), for a total of 38 expeditions covering all seasons. Taxonomic treatment of the resulting specimens includes identification keys, diagnoses and descriptions, conservation status, endemism and examined material. A total of 59 species were found, belonging to 32 genera and 10 tribes. The predominant genera were Lessingianthus (8 spp.) and Chromolaena (5 spp.) while the predominant tribes were Vernonieae (19 spp.), Eupatorieae (13 spp.) and Heliantheae (7 spp.). The richness pattern for tribes was the same as recorded in other floristic inventories in the Cerrado domain, especially cerrado sensu stricto. Twenty-one of the species (35.6%) are restricted to Brazil while only Isostigma resupinatum is restricted to the state of Goiás. Conyza primulifolia, Lepidaploa cuiabensis and Lepidaploa sororia were new occurrences for the state.
DOI: 10.6084/m9.figshare.22785495.v1
title: Asteraceae of Serra da Confusão do Rio Preto, Quirinópolis, Goiás, Brazil

description: Abstract A floristic inventory and a taxonomic treatment of Asteraceae from Serra da Confusão do Rio Preto in the state of Goiás are provided. Collections were carried out from July 2017 to December 2018 in a fragment of cerrado sensu stricto with transition to cerrado rupestre (rocky cerrado), for a total of 38 expeditions covering all seasons. Taxonomic treatment of the resulting specimens includes identification keys, diagnoses and descriptions, conservation status, endemism and examined material. A total of 59 species were found, belonging to 32 genera and 10 tribes. The predominant genera were Lessingianthus (8 spp.) and Chromolaena (5 spp.) while the predominant tribes were Vernonieae (19 spp.), Eupatorieae (13 spp.) and Heliantheae (7 spp.). The richness pattern for tribes was the same as recorded in other floristic inventories in the Cerrado domain, especially cerrado sensu stricto. Twenty-one of the species (35.6%) are restricted to Brazil while only Isostigma resupinatum is restricted to the state of Goiás. Conyza primulifolia, Lepidaploa cuiabensis and Lepidaploa sororia were new occurrences for the state.
DOI: 10.25919/5q31-xj77
title: Parkes observations for project P456 semester 2023APRS_02

description: The Parkes Pulsar Timing Array (PPTA) project has three primary goals: (a) detection of gravitational waves from astronomical sources, (b) establishment of a pulsar timescale, and (c) improvement of our understanding of Solar-system dynamics. We are now in an exciting regime where a signal detected in our (and other data sets) has the form expected for a gravitational wave background. We are therefore now attempting to confirm, or deny, this exciting result. With this proposal we aim to maintain our pre-eminent position in the field. Unlike most observing proposals, this is a continuing proposal for which the observations will continue to improve bounds on ultra-low-frequency gravitational waves until they are finally detected. Continued Parkes observations will remain valuable at least until the first stage of the SKA is able to improve on our sensitivity and observing cadence. Even after the gravitational wave background is detected we will want to continue observations in support of the nascent field of gravitational wave astronomy!
DOI: 10.6084/m9.figshare.22785496
title: Qualitative vulnerability of the waters of a surface reservoir subjected to drought in a tropical semi-arid region

description: ABSTRACT Irregular rainfall and the occurrence of droughts in tropical semi-arid regions have a negative impact on the quality and availability of water stored in reservoirs. The aim of this study was to evaluate the quality of water stored in an artificial reservoir in the tropical semi-arid region of Brazil during years of drought. The study area was the Pereira de Miranda reservoir, located in the district of Pentecoste, Ceará, Brazil. Seven water-sampling campaigns were carried out between April 2015 and September 2016. The variables to be analysed were chlorophyll-a (Cl-a), hydrogen potential (pH), electrical conductivity (EC), total nitrogen (Ntotal), total phosphorus (Ptotal), transparency (SD), fixed/volatile/total suspended solids (FSS/VSS/TSS), and temperature (T0); the trophic state index (TSI) was then calculated. The variables which better explained the variance in water quality were identified by principal component analysis (PCA). The waters of the Pereira de Miranda reservoir were classified as hyper-eutrophic throughout the study period and at each of the collection points. The PCA identified that the variability in water quality was determined by suspended sediment and nutrients, showing that the reduction in variables made it possible to obtain high explainability of the conditions of the reservoir during monitoring, saving time and resources on the analyses. In addition, the occurrence of severe drought resulted in low accumulated volumes, which intensified and made vulnerable the quality of the waters. As such, the Pereira de Miranda reservoir proved to be in a process of degradation, mainly due to anthropogenic action, intensified by climate factors.
DOI: 10.6084/m9.figshare.22785496.v1
title: Qualitative vulnerability of the waters of a surface reservoir subjected to drought in a tropical semi-arid region

description: ABSTRACT Irregular rainfall and the occurrence of droughts in tropical semi-arid regions have a negative impact on the quality and availability of water stored in reservoirs. The aim of this study was to evaluate the quality of water stored in an artificial reservoir in the tropical semi-arid region of Brazil during years of drought. The study area was the Pereira de Miranda reservoir, located in the district of Pentecoste, Ceará, Brazil. Seven water-sampling campaigns were carried out between April 2015 and September 2016. The variables to be analysed were chlorophyll-a (Cl-a), hydrogen potential (pH), electrical conductivity (EC), total nitrogen (Ntotal), total phosphorus (Ptotal), transparency (SD), fixed/volatile/total suspended solids (FSS/VSS/TSS), and temperature (T0); the trophic state index (TSI) was then calculated. The variables which better explained the variance in water quality were identified by principal component analysis (PCA). The waters of the Pereira de Miranda reservoir were classified as hyper-eutrophic throughout the study period and at each of the collection points. The PCA identified that the variability in water quality was determined by suspended sediment and nutrients, showing that the reduction in variables made it possible to obtain high explainability of the conditions of the reservoir during monitoring, saving time and resources on the analyses. In addition, the occurrence of severe drought resulted in low accumulated volumes, which intensified and made vulnerable the quality of the waters. As such, the Pereira de Miranda reservoir proved to be in a process of degradation, mainly due to anthropogenic action, intensified by climate factors.
DOI: 10.17632/zgr9bj57r4.1
title: snRNAseq- Neuroectodermal IL-12R signaling in neuroinflammation/EAE

description: We performed single-nucleus RNA sequencing (Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 protocol, 10X Genomics) of FANS-isolated Hoechst+ nuclei from the cerebellum, brainstem and cervical spinal cord of NestinCre/+Il12rb2fl/fl mice and their Il12rb2fl/fl littermates at the steady-state and onset of clinical EAE symptoms (10 dpi) . 
We performed two independent sequencing experiments and successfully sequenced 5 samples. The first experiment included two samples: Il12rb2fl_naive, NestinCre_Il12rb2fl_naive.  The second experiment included three samples: Il12rb2fl_EAE, NestinCre_Il12rb2fl_EAE and Il12rb2_naive_AUG. Sample Il12rb2_naive_AUG was only an internal control to assess reproducibility and batch effects between the two sequencing experiments and is excluded from the final visualizations displayed in our manuscript. After quality control and doublet exclusion, snRNA-seq yielded a total of 87,076 single-nucleus transcriptomic profiles, among which 22,018 distinct genes were detected. Unsupervised clustering of these data identified 16 clusters that were assigned to diverse neuronal, glial and other cell types on the basis of known lineage marker genes. This allowed us to assess how the transcriptional profile of the neuroectoderm differed in the presence or absence of IL-12 receptor signaling by interrogating differentially expressed genes (DEGs). This revealed pronounced alterations particularly in excitatory neurons, granule cells, mature oligodendrocytes (MOLs) and myelin forming oligodendrocytes (MFOLs1) suggesting a specific/concentrated action of IL-12 on these populations in the inflamed CNS. 
Our data suggest IL-12 to be critically involved in neuroprotection and shaping the trophic factor milieu within the inflamed CNS. Conversely, dysregulation or loss of trophic factor release by neurons in the absence of IL-12 may further propagate the degeneration of neurons, oligodendrocytes and possibly other cell types. 

Sequencing raw data and processed gene expression data will be deposited into the GEO repository upon publication. Code for analyses will be available upon contacting the corresponding authors: becher@immunology.uzh.ch and mundt@immunology.uzh.ch
DOI: 10.17632/zgr9bj57r4.2
title: snRNAseq- Neuroectodermal IL-12R signaling in neuroinflammation/EAE

description: We performed single-nucleus RNA sequencing (Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 protocol, 10X Genomics) of FANS-isolated Hoechst+ nuclei from the cerebellum, brainstem and cervical spinal cord of NestinCre/+Il12rb2fl/fl mice and their Il12rb2fl/fl littermates at the onset of clinical EAE symptoms (10 dpi) . 
We performed two independent sequencing experiments and successfully sequenced 8 samples. The first experiment included two samples: Il12rb2fl, NestinCre_Il12rb2fl.  The second experiment included six samples: three Il12rb2fl mice and three NestinCre_Il12rb2fl mice. After quality control and doublet exclusion, snRNA-seq yielded a total of 133,151 single-nucleus transcriptomic profiles, among which 21,552 distinct genes were detected. Batches were integrated using SCVI. Unsupervised clustering of these data identified clusters that were assigned to diverse neuronal, glial and other cell types on the basis of known lineage marker genes. This allowed us to assess how the transcriptional profile of the neuroectoderm differed in the presence or absence of IL-12 receptor signaling by interrogating differentially expressed genes (DEGs). This revealed pronounced alterations particularly in excitatory neurons, granule cells, mature oligodendrocytes (MOLs) and myelin forming oligodendrocytes (MOLs1) suggesting a specific/concentrated action of IL-12 on these populations in the inflamed CNS. 
Our data suggest IL-12 to be critically involved in neuroprotection and shaping the trophic factor milieu within the inflamed CNS. Conversely, dysregulation or loss of trophic factor release by neurons in the absence of IL-12 may further propagate the degeneration of neurons, oligodendrocytes and possibly other cell types. 

Sequencing raw data and processed gene expression data will be deposited into the GEO repository upon publication. Code for analyses will be available upon contacting the corresponding authors: becher@immunology.uzh.ch and mundt@immunology.uzh.ch
DOI: 10.6084/m9.figshare.22785498
title: How do impeachments influence foreign policies? Lessons from South America

description: Abstract Although we have a vast understanding of the causes and institutional impacts of impeachments, we know little about their policy outcomes. As such events occur during crises, new incumbents are expected to promote policy reforms. To identify how they influence foreign policy, I applied a most-different-system design to compare the cases of Lugo in Paraguay (2012) and Rousseff in Brazil (2016). Internally, impeachments politicize foreign policy, linking the external agenda to domestic politics. Externally, they may restrict foreign policy alternatives, depending on political actors’ perceptions of process legitimacy, ideological proximity, and state resources. I found that politicization after impeachments does not necessarily bring decision-making decentralization. Instead, actors capture the foreign policy agenda for political purposes.
DOI: 10.6084/m9.figshare.22785498.v1
title: How do impeachments influence foreign policies? Lessons from South America

description: Abstract Although we have a vast understanding of the causes and institutional impacts of impeachments, we know little about their policy outcomes. As such events occur during crises, new incumbents are expected to promote policy reforms. To identify how they influence foreign policy, I applied a most-different-system design to compare the cases of Lugo in Paraguay (2012) and Rousseff in Brazil (2016). Internally, impeachments politicize foreign policy, linking the external agenda to domestic politics. Externally, they may restrict foreign policy alternatives, depending on political actors’ perceptions of process legitimacy, ideological proximity, and state resources. I found that politicization after impeachments does not necessarily bring decision-making decentralization. Instead, actors capture the foreign policy agenda for political purposes.
DOI: 10.6084/m9.figshare.22785499
title: Histogram analysis in the differentiation between adrenal adenomas and pheochromocytomas: the value of a single measurement

description: Abstract Objective: To assess the diagnostic accuracy of histogram analysis on unenhanced computed tomography (CT) for differentiating between adrenal adenomas and pheochromocytomas (PCCs). Materials and Methods: We retrospectively identified patients with proven PCCs who had undergone CT examinations between January 2009 and July 2019 at one of two institutions. For each PCC, we selected one or two adenomas diagnosed within two weeks of the date of diagnosis of the PCC. For each lesion, two readers scored the size, determined the mean attenuation, and generated a voxel histogram. The 10th percentile (P10) was obtained from the conventional histogram analysis, as well as being calculated with the following formula: P10 = mean attenuation – (1.282 × standard deviation). The mean attenuation threshold, histogram analysis (observed) P10, and calculated P10 (calcP10) were compared in terms of their diagnostic accuracy. Results: We included 52 adenomas and 29 PCCs. The sensitivity, specificity, and accuracy of the mean attenuation threshold were 75.0%, 100.0%, and 82.5%, respectively, for reader 1, whereas they were 71.5%, 100.0%, and 81.5%, respectively, for reader 2. The sensitivity, specificity, and accuracy of the observed P10 and calcP10 were equal for both readers: 90.4%, 96.5%, and 92.6%, respectively, for reader 1; and 92.3%, 93.1%, and 92.6%, respectively, for reader 2. The increase in sensitivity was significant for both readers (p = 0.009 and p = 0.005, respectively). Conclusion: For differentiating between adenomas and PCCs, the histogram analysis (observed P10 and calcP10) appears to outperform the mean attenuation threshold as a diagnostic criterion.
DOI: 10.6084/m9.figshare.22785499.v1
title: Histogram analysis in the differentiation between adrenal adenomas and pheochromocytomas: the value of a single measurement

description: Abstract Objective: To assess the diagnostic accuracy of histogram analysis on unenhanced computed tomography (CT) for differentiating between adrenal adenomas and pheochromocytomas (PCCs). Materials and Methods: We retrospectively identified patients with proven PCCs who had undergone CT examinations between January 2009 and July 2019 at one of two institutions. For each PCC, we selected one or two adenomas diagnosed within two weeks of the date of diagnosis of the PCC. For each lesion, two readers scored the size, determined the mean attenuation, and generated a voxel histogram. The 10th percentile (P10) was obtained from the conventional histogram analysis, as well as being calculated with the following formula: P10 = mean attenuation – (1.282 × standard deviation). The mean attenuation threshold, histogram analysis (observed) P10, and calculated P10 (calcP10) were compared in terms of their diagnostic accuracy. Results: We included 52 adenomas and 29 PCCs. The sensitivity, specificity, and accuracy of the mean attenuation threshold were 75.0%, 100.0%, and 82.5%, respectively, for reader 1, whereas they were 71.5%, 100.0%, and 81.5%, respectively, for reader 2. The sensitivity, specificity, and accuracy of the observed P10 and calcP10 were equal for both readers: 90.4%, 96.5%, and 92.6%, respectively, for reader 1; and 92.3%, 93.1%, and 92.6%, respectively, for reader 2. The increase in sensitivity was significant for both readers (p = 0.009 and p = 0.005, respectively). Conclusion: For differentiating between adenomas and PCCs, the histogram analysis (observed P10 and calcP10) appears to outperform the mean attenuation threshold as a diagnostic criterion.
DOI: 10.6084/m9.figshare.22785501.v1
title: Development and validation of liquid chromatography-tandem mass spectrometry method to quantify dasatinib in plasma and its application to a pharmacokinetic study

description: Abstract Dasatinib, a potent oral multi-targeted kinase inhibitor against Src and Bcr-Abl, can decrease inflammatory response in sepsis. A simple and cost-effective method for determination of an effective dose dasatinib was established. This method was validated in human plasma, with the aim of reducing the number of animals used, thus, avoiding ethical problems. Dasatinib and internal standard lopinavir were extracted from 180 uL of plasma using liquid-liquid extraction with methyl tert-butil ether, followed by liquid chromatography coupled to triple quadrupole mass spectrometry in multiple reaction monitoring mode. For the pharmacokinetic study, 1 mg/kg of dasatinib was administered to mice with and without sepsis. The method was linear over the concentration range of 1-98 ng/mL for DAS in mice and human plasma, with r2&gt;0.99 and presented intra- and interday precision within the range of 2.3 - 6.2 and 4.3 - 7.0%, respectively. Further intra- and interday accuracy was within the range of 88.2 - 105.8 and 90.6 - 101.7%, respectively. The mice with sepsis showed AUC0-t = 2076.06 h*ng/mL and Cmax =102.73 ng/mL and mice without sepsis presented AUC0-t = 2128.46 h*ng/mL. Cmax = 164.5 ng/mL. The described analytical method was successfully employed in pharmacokinetic study of DAS in mice.
DOI: 10.6084/m9.figshare.22785501
title: Development and validation of liquid chromatography-tandem mass spectrometry method to quantify dasatinib in plasma and its application to a pharmacokinetic study

description: Abstract Dasatinib, a potent oral multi-targeted kinase inhibitor against Src and Bcr-Abl, can decrease inflammatory response in sepsis. A simple and cost-effective method for determination of an effective dose dasatinib was established. This method was validated in human plasma, with the aim of reducing the number of animals used, thus, avoiding ethical problems. Dasatinib and internal standard lopinavir were extracted from 180 uL of plasma using liquid-liquid extraction with methyl tert-butil ether, followed by liquid chromatography coupled to triple quadrupole mass spectrometry in multiple reaction monitoring mode. For the pharmacokinetic study, 1 mg/kg of dasatinib was administered to mice with and without sepsis. The method was linear over the concentration range of 1-98 ng/mL for DAS in mice and human plasma, with r2&gt;0.99 and presented intra- and interday precision within the range of 2.3 - 6.2 and 4.3 - 7.0%, respectively. Further intra- and interday accuracy was within the range of 88.2 - 105.8 and 90.6 - 101.7%, respectively. The mice with sepsis showed AUC0-t = 2076.06 h*ng/mL and Cmax =102.73 ng/mL and mice without sepsis presented AUC0-t = 2128.46 h*ng/mL. Cmax = 164.5 ng/mL. The described analytical method was successfully employed in pharmacokinetic study of DAS in mice.
DOI: 10.6084/m9.figshare.22785504
title: DEVELOPMENT OF A METHODOLOGY FOR THE DETERMINATION OF CANNABINOIDS IN CANNABIS-BASED PRODUCTS FOR MEDICINAL PURPOSES

description: Medicinal products based on the Cannabis sativa plant are available in Brazil, among them, drug, cannabis products, individual import products, and those provided by patient associations. Despite this, there is no monograph for the evaluation of cannabinoids in Brazilian Pharmacopoeia (BP) or other compendia accepted. Therefore, this work aimed to develop a quick and easy method to quantify cannabinoids in authorized products for medicinal purposes. The method was developed by HPLC-UV, gradient elution composed of formic acid in acetonitrile and water, the flow rate of 0.6 mL min-1 and 220 nm detection. It was possible to resolve the peaks of cannabidiol (CBD), tetrahydrocannabinol (THC), cannabidiolic acid and cannabinol and to quantify CBD (105.35%; 104.07%) and THC (106.62%). The developed method was applied to two products and met the established requirements of precision, accuracy, linearity, and selectivity. This procedure has been tested on four cannabis-based products to attest to its effectiveness. Considering the importance of establishing minimum quality requirements for medicinal products and considering the available products in Brazil, it was found that it is important to evaluate the implementation of other products in the method developed for future validation and later inclusion in the BP.
DOI: 10.6084/m9.figshare.22785504.v1
title: DEVELOPMENT OF A METHODOLOGY FOR THE DETERMINATION OF CANNABINOIDS IN CANNABIS-BASED PRODUCTS FOR MEDICINAL PURPOSES

description: Medicinal products based on the Cannabis sativa plant are available in Brazil, among them, drug, cannabis products, individual import products, and those provided by patient associations. Despite this, there is no monograph for the evaluation of cannabinoids in Brazilian Pharmacopoeia (BP) or other compendia accepted. Therefore, this work aimed to develop a quick and easy method to quantify cannabinoids in authorized products for medicinal purposes. The method was developed by HPLC-UV, gradient elution composed of formic acid in acetonitrile and water, the flow rate of 0.6 mL min-1 and 220 nm detection. It was possible to resolve the peaks of cannabidiol (CBD), tetrahydrocannabinol (THC), cannabidiolic acid and cannabinol and to quantify CBD (105.35%; 104.07%) and THC (106.62%). The developed method was applied to two products and met the established requirements of precision, accuracy, linearity, and selectivity. This procedure has been tested on four cannabis-based products to attest to its effectiveness. Considering the importance of establishing minimum quality requirements for medicinal products and considering the available products in Brazil, it was found that it is important to evaluate the implementation of other products in the method developed for future validation and later inclusion in the BP.