cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4056555.v1
title: Supplementary Material for: Histaminergic H3-Heteroreceptors as a Potential Mediator of Betahistine-Induced Increase in Cochlear Blood Flow

description: <br><strong><em>Objective:</em></strong> Betahistine is a histamine-like drug that is considered beneficial in Ménière's disease by increasing cochlear blood flow. Acting as an agonist at the histamine H<sub>1</sub>-receptor and as an inverse agonist at the H<sub>3</sub>-receptor, these receptors as well as the adrenergic α<sub>2</sub>-receptor were investigated for betahistine effects on cochlear blood flow. <b><i>Materials and Methods:</i></b> A total of 54 Dunkin-Hartley guinea pigs were randomly assigned to one of nine groups treated with a selection of H<sub>1</sub>-, H<sub>3</sub>- or α<sub>2</sub>-selective agonists and antagonists together with betahistine. Cochlear blood flow and mean arterial pressure were recorded for 3 min before and 15 min after infusion. <b><i>Results:</i></b> Blockage of the H<sub>3</sub>- or α<sub>2</sub>-receptors caused a suppression of betahistine-mediated typical changes in cochlear blood flow or blood pressure. Activation of H<sub>3</sub>-receptors caused a drop in cochlear blood flow and blood pressure. H<sub>1</sub>-receptors showed no involvement in betahistine-mediated changes of cochlear blood flow. <b><i>Conclusion:</i></b> Betahistine most likely affects cochlear blood flow through histaminergic H<sub>3</sub>-heteroreceptors.
DOI: 10.7910/dvn/v1rjpf/dsacij
title: blc20151009_03.txt.gz

description: :unav
DOI: 10.7910/dvn/v1rjpf/2yxp0i
title: blc20151024_03.txt.gz

description: :unav
DOI: 10.7910/dvn/v1rjpf/kj0n6t
title: blc20150315_03.txt.gz

description: :unav
DOI: 10.7910/dvn/v1rjpf/xfkrxs
title: blc20151124_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056528
title: Supplementary Material for: Effect of Different Respiratory Modes on Return of Spontaneous Circulation in a Newborn Piglet Model of Hypoxic Cardiac Arrest

description: <br><strong><em>Background:</em></strong> There are no clear evidence-based recommendations on the use of different techniques of respiratory support and chest compressions (CC) during neonatal cardiopulmonary resuscitation (CPR). <b><i>Objectives:</i></b> To determine the effects of different respiratory support strategies along with CC representing clinical practice on the return of spontaneous circulation (ROSC) in hypoxic newborn piglets with cardiac arrest. We hypothesized that use of a T-piece resuscitator (TPR) providing positive end-expiratory pressure (PEEP) reduces time to ROSC as compared to a self-inflating bag (SIB) without PEEP. Furthermore, we explored the effects of a ventilator providing inflations without synchrony to CC. <b><i>Methods:</i></b> Thirty-three newborn piglets were exposed to hypoxia until asystole occurred and randomized into three groups and resuscitated according to ILCOR guidelines: group 1 = TPR [peak inspiratory pressure (PIP)/PEEP of 25/5 cm H<sub>2</sub>O, rate 30/min], inflations interposed between CC (3:1 ratio); group 2 = SIB (PIP of 25 cm H<sub>2</sub>O without PEEP, rate 30/min), inflations interposed between CC (3:1 ratio), and group 3 = ventilator (PIP/PEEP of 25/5 cm H<sub>2</sub>O, rate 30/min), CC were applied with a rate of 120/min without synchrony to inflations. Animals were supported for 120 min after ROSC. Primary outcome was time to ROSC. <b><i>Results:</i></b> All animals achieved ROSC. We found no significant difference in time to ROSC between groups [median (IQR); TPR: 150 s (150-210); SIB: 150 s (120-180); ventilator: 180 s (150-345)]. There was no difference in use of epinephrine, in blood gases or hemodynamic parameters during the 120-min observation time after ROSC. <b><i>Conclusions:</i></b> We found no significant effect of different respiratory support strategies during CPR on ROSC.
DOI: 10.7910/dvn/v1rjpf/cmcibd
title: blc20150801_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056528.v1
title: Supplementary Material for: Effect of Different Respiratory Modes on Return of Spontaneous Circulation in a Newborn Piglet Model of Hypoxic Cardiac Arrest

description: <br><strong><em>Background:</em></strong> There are no clear evidence-based recommendations on the use of different techniques of respiratory support and chest compressions (CC) during neonatal cardiopulmonary resuscitation (CPR). <b><i>Objectives:</i></b> To determine the effects of different respiratory support strategies along with CC representing clinical practice on the return of spontaneous circulation (ROSC) in hypoxic newborn piglets with cardiac arrest. We hypothesized that use of a T-piece resuscitator (TPR) providing positive end-expiratory pressure (PEEP) reduces time to ROSC as compared to a self-inflating bag (SIB) without PEEP. Furthermore, we explored the effects of a ventilator providing inflations without synchrony to CC. <b><i>Methods:</i></b> Thirty-three newborn piglets were exposed to hypoxia until asystole occurred and randomized into three groups and resuscitated according to ILCOR guidelines: group 1 = TPR [peak inspiratory pressure (PIP)/PEEP of 25/5 cm H<sub>2</sub>O, rate 30/min], inflations interposed between CC (3:1 ratio); group 2 = SIB (PIP of 25 cm H<sub>2</sub>O without PEEP, rate 30/min), inflations interposed between CC (3:1 ratio), and group 3 = ventilator (PIP/PEEP of 25/5 cm H<sub>2</sub>O, rate 30/min), CC were applied with a rate of 120/min without synchrony to inflations. Animals were supported for 120 min after ROSC. Primary outcome was time to ROSC. <b><i>Results:</i></b> All animals achieved ROSC. We found no significant difference in time to ROSC between groups [median (IQR); TPR: 150 s (150-210); SIB: 150 s (120-180); ventilator: 180 s (150-345)]. There was no difference in use of epinephrine, in blood gases or hemodynamic parameters during the 120-min observation time after ROSC. <b><i>Conclusions:</i></b> We found no significant effect of different respiratory support strategies during CPR on ROSC.
DOI: 10.6084/m9.figshare.4056522
title: Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum

description: <em>Species in the genus Cercospora cause economically devastating diseases in sugar beet, maize, rice, soy bean and other major food crops</em>. Here we sequenced the genome of the sugar beet pathogen <i>C. beticola<em> and found it </em></i>encodes 63 putative secondary metabolite gene clusters, including the cercosporin toxin biosynthesis (<i>CTB</i>) cluster. We show that the <em>CTB gene cluster</em> has experienced multiple duplications and horizontal transfers across a spectrum of plant pathogenic fungi, including the wide-host range <i>Colletotrichum</i> genus as well as the rice pathogen <i>Magnaporthe oryzae</i>. Although cercosporin biosynthesis has been thought to-date to rely on an eight gene <i>CTB</i> cluster, our phylogenomic analysis revealed gene collinearity adjacent to the established cluster in all <i>CTB </i>cluster-harboring species. We demonstrate that the <i>CTB</i> cluster is larger than previously recognized and includes <i>cercosporin facilitator protein </i>(<i>CFP</i>), previously shown to be involved with cercosporin auto-resistance, and four additional genes required for cercosporin biosynthesis, including the final pathway enzymes that install the unusual cercosporin methylenedioxy bridge. Finally, we demonstrate production of cercosporin by <i>Colletotrichum fioriniae</i>, the first known cercosporin producer within this agriculturally important genus. Thus, our results provide new insight into the intricate evolution and biology of a toxin critical to agriculture and broaden the production of cercosporin to another fungal genus containing many plant pathogens of important crops worldwide.
DOI: 10.7910/dvn/v1rjpf/rxqqud
title: blc20150808_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056525
title: Supplementary Material for: Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes

description: <br>5S and 45S rDNA sites are the best mapped chromosome regions in eukaryotic chromosomes. In this work, a database was built gathering information about the position and number of 5S rDNA sites in 784 plant species, aiming to identify patterns of distribution along the chromosomes and its correlation with the position of 45S rDNA sites. Data revealed that in most karyotypes (54.5%, including polyploids) two 5S rDNA sites (a single pair) are present, with 58.7% of all sites occurring in the short arm, mainly in the proximal region. In karyotypes of angiosperms with only 1 pair of sites (single sites) they are mostly found in the proximal region (52.0%), whereas in karyotypes with multiple sites the location varies according to the average chromosome size. Karyotypes with multiple sites and small chromosomes (&lt;3 µm) often display proximal sites, while medium-sized (between 3 and 6 µm) and large chromosomes (&gt;6 µm) more commonly show terminal or interstitial sites. In species with holokinetic chromosomes, the modal value of sites per karyotype was also 2, but they were found mainly in a terminal position. Adjacent 5S and 45S rDNA sites were often found in the short arm, reflecting the preferential distribution of both sites in this arm. The high frequency of genera with at least 1 species with adjacent 5S and 45S sites reveals that this association appeared several times during angiosperm evolution, but it has been maintained only rarely as the dominant array in plant genera.
DOI: 10.7910/dvn/v1rjpf/mycz1i
title: blc20150514_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056522.v2
title: Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum

description: <em>Species in the genus Cercospora cause economically devastating diseases in sugar beet, maize, rice, soy bean and other major food crops</em>. Here we sequenced the genome of the sugar beet pathogen <i>C. beticola<em> and found it </em></i>encodes 63 putative secondary metabolite gene clusters, including the cercosporin toxin biosynthesis (<i>CTB</i>) cluster. We show that the <em>CTB gene cluster</em> has experienced multiple duplications and horizontal transfers across a spectrum of plant pathogenic fungi, including the wide-host range <i>Colletotrichum</i> genus as well as the rice pathogen <i>Magnaporthe oryzae</i>. Although cercosporin biosynthesis has been thought to-date to rely on an eight gene <i>CTB</i> cluster, our phylogenomic analysis revealed gene collinearity adjacent to the established cluster in all <i>CTB </i>cluster-harboring species. We demonstrate that the <i>CTB</i> cluster is larger than previously recognized and includes <i>cercosporin facilitator protein </i>(<i>CFP</i>), previously shown to be involved with cercosporin auto-resistance, and four additional genes required for cercosporin biosynthesis, including the final pathway enzymes that install the unusual cercosporin methylenedioxy bridge. Finally, we demonstrate production of cercosporin by <i>Colletotrichum fioriniae</i>, the first known cercosporin producer within this agriculturally important genus. Thus, our results provide new insight into the intricate evolution and biology of a toxin critical to agriculture and broaden the production of cercosporin to another fungal genus containing many plant pathogens of important crops worldwide.
DOI: 10.6084/m9.figshare.4056525.v1
title: Supplementary Material for: Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes

description: <br>5S and 45S rDNA sites are the best mapped chromosome regions in eukaryotic chromosomes. In this work, a database was built gathering information about the position and number of 5S rDNA sites in 784 plant species, aiming to identify patterns of distribution along the chromosomes and its correlation with the position of 45S rDNA sites. Data revealed that in most karyotypes (54.5%, including polyploids) two 5S rDNA sites (a single pair) are present, with 58.7% of all sites occurring in the short arm, mainly in the proximal region. In karyotypes of angiosperms with only 1 pair of sites (single sites) they are mostly found in the proximal region (52.0%), whereas in karyotypes with multiple sites the location varies according to the average chromosome size. Karyotypes with multiple sites and small chromosomes (&lt;3 µm) often display proximal sites, while medium-sized (between 3 and 6 µm) and large chromosomes (&gt;6 µm) more commonly show terminal or interstitial sites. In species with holokinetic chromosomes, the modal value of sites per karyotype was also 2, but they were found mainly in a terminal position. Adjacent 5S and 45S rDNA sites were often found in the short arm, reflecting the preferential distribution of both sites in this arm. The high frequency of genera with at least 1 species with adjacent 5S and 45S sites reveals that this association appeared several times during angiosperm evolution, but it has been maintained only rarely as the dominant array in plant genera.
DOI: 10.7910/dvn/v1rjpf/t8atwv
title: blc20151101_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056519
title: Supplementary Material for: Perioperative Blood Transfusion, Age at Surgery, and Prognosis in a Database of 526 Upper Gastrointestinal Cancers

description: <br><strong><em>Aims:</em></strong> It is demonstrated that older animals have significantly weaker responses to new alloantigen stimulation than young animals, but the effect on prognosis of perioperative blood transfusion in relation to patient age is unknown. This study is retrospective review to investigate the relationship between perioperative blood transfusion, age at surgery, and clinical outcome in upper gastrointestinal cancer patients. <b><i>Methods:</i></b> We analyzed data of 526 upper gastrointestinal cancer patients who underwent curative resection from 2005 to 2010. <b><i>Results:</i></b> In esophageal cancer patients, patients with blood transfusion experienced significantly shorter overall survival (OS; univariate HR 2.50, p = 0.0006) and disease-free survival (DFS; univariate HR 1.71, p = 0.016) than patients without. Similar results were observed in gastric cancer patients (OS; univariate HR 3.35, p = 0.0001 and DFS; univariate HR = 3.18, p &lt; 0.0001). Furthermore perioperative blood transfusion may be an independent prognostic factor in esophageal cancer patients (multivariate HR = 2.07, p = 0.026). Interestingly, age at surgery significantly affected the influence of blood transfusion on patient outcome in esophageal cancer patients (p for interaction = 0.022). <b><i>Conclusion:</i></b> The negative effect of perioperative blood transfusion was particularly evident among younger patients with esophageal cancer.
DOI: 10.6084/m9.figshare.4056522.v1
title: Conservation of a gene cluster reveals novel cercosporin biosynthetic mechanisms and extends production to the genus Colletotrichum

description: Species in the genus Cercospora cause economically devastating diseases in sugar beet, maize, rice, soy bean and other major food crops. Here we sequenced the genome of the sugar beet pathogen C. beticola and found it encodes 63 putative secondary metabolite gene clusters, including the cercosporin toxin biosynthesis (CTB) cluster. We show that the CTB gene cluster has experienced multiple duplications and horizontal transfers across a spectrum of plant pathogenic fungi, including the wide-host range Colletotrichum genus as well as the rice pathogen Magnaporthe oryzae. Although cercosporin biosynthesis has been thought to-date to rely on an eight gene CTB cluster, our phylogenomic analysis revealed gene collinearity adjacent to the established cluster in all CTB cluster-harboring species. We demonstrate that the CTB cluster is larger than previously recognized and includes cercosporin facilitator protein (CFP) previously shown to be involved with cercosporin auto-resistance, and four additional genes required for cercosporin biosynthesis including the final pathway enzymes that install the unusual cercosporin methylenedioxy bridge. Finally, we demonstrate production of cercosporin by Colletotrichum fioriniae, the first known cercosporin producer within this agriculturally important genus. Thus, our results provide new insight into the intricate evolution and biology of a toxin critical to agriculture and broaden the production of cercosporin to another fungal genus containing many plant pathogens of important crops worldwide.
DOI: 10.6084/m9.figshare.4056516
title: Supplementary Material for: Formamide-Free Genomic in situ Hybridization Allows Unambiguous Discrimination of Highly Similar Parental Genomes in Diploid Hybrids and Allopolyploids

description: <br>Polyploidy and hybridization play an important role in plant diversification and speciation. The application of genomic in situ hybridization (GISH) allows the identification of parental genomes in hybrids, thus elucidating their origins and allowing for analysis of their genomic evolution. The performance of GISH depends on the similarity of the parental genomes and on the age of hybrids. Here, we present the formamide-free GISH (ff-GISH) protocol applied to diploid and polyploid hybrids of monocots (<i>Prospero</i>, Hyacinthaceae) and dicots (<i>Melampodium</i>, Asteraceae) differing in similarity of the parental genomes and in chromosome and genome sizes. The efficiency of the new protocol is compared to the standard GISH protocol. As a result, ff-GISH allowed efficient labeling and discrimination of the parental chromosome sets in diploid and allopolyploid hybrids in <i>Prospero autumnale</i> species complex. In contrast, the standard GISH protocol failed to differentiate the parental genomes due to high levels of similar repetitive DNA. Likewise, an unambiguous identification of parental genomes in allotetraploid <i>Melampodium nayaritense</i> (Asteraceae) was possible after ff-GISH, whereas the standard GISH hybridization performance was suboptimal. The modified method is simple and non-toxic and allows the discrimination of very similar parental genomes in hybrids. This method lends itself to modifications and improvements and can also be used for FISH.
DOI: 10.7910/dvn/v1rjpf/n6r2og
title: blc20150326_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056513
title: Latent semantic analysis cosines as a cognitive similarity measure: Evidence from priming studies

description: Data sets, variable descriptions and R scripts for the following article:<br> Günther, F., Dudschig, C., & Kaup, B. (2016). Latent Semantic Analysis cosines as a cognitive similarity measure: Evidence from priming studies. <i>Quarterly Journal of Experimental Psychology, 69, </i>626-653<i>.</i>doi: http://dx.doi.org/10.1080/17470218.2015.1038280<i><br></i> <br>
DOI: 10.6084/m9.figshare.4056516.v1
title: Supplementary Material for: Formamide-Free Genomic in situ Hybridization Allows Unambiguous Discrimination of Highly Similar Parental Genomes in Diploid Hybrids and Allopolyploids

description: <br>Polyploidy and hybridization play an important role in plant diversification and speciation. The application of genomic in situ hybridization (GISH) allows the identification of parental genomes in hybrids, thus elucidating their origins and allowing for analysis of their genomic evolution. The performance of GISH depends on the similarity of the parental genomes and on the age of hybrids. Here, we present the formamide-free GISH (ff-GISH) protocol applied to diploid and polyploid hybrids of monocots (<i>Prospero</i>, Hyacinthaceae) and dicots (<i>Melampodium</i>, Asteraceae) differing in similarity of the parental genomes and in chromosome and genome sizes. The efficiency of the new protocol is compared to the standard GISH protocol. As a result, ff-GISH allowed efficient labeling and discrimination of the parental chromosome sets in diploid and allopolyploid hybrids in <i>Prospero autumnale</i> species complex. In contrast, the standard GISH protocol failed to differentiate the parental genomes due to high levels of similar repetitive DNA. Likewise, an unambiguous identification of parental genomes in allotetraploid <i>Melampodium nayaritense</i> (Asteraceae) was possible after ff-GISH, whereas the standard GISH hybridization performance was suboptimal. The modified method is simple and non-toxic and allows the discrimination of very similar parental genomes in hybrids. This method lends itself to modifications and improvements and can also be used for FISH.
DOI: 10.6084/m9.figshare.4056513.v1
title: Latent semantic analysis cosines as a cognitive similarity measure: Evidence from priming studies

description: Data sets, variable descriptions and R scripts for the following article:<br> Günther, F., Dudschig, C., & Kaup, B. (2016). Latent Semantic Analysis cosines as a cognitive similarity measure: Evidence from priming studies. <i>Quarterly Journal of Experimental Psychology, 69, </i>626-653<i>.</i>doi: http://dx.doi.org/10.1080/17470218.2015.1038280<i><br></i> <br>
DOI: 10.7910/dvn/v1rjpf/1hpxt0
title: blc20150202_03.txt.gz

description: :unav
DOI: 10.6084/m9.figshare.4056504
title: Supplementary Material for: Paricalcitol, Microvascular and Endothelial Function in Non-Diabetic Chronic Kidney Disease: A Randomized Trial

description: <br><strong><em>Background:</em></strong> Vitamin D deficiency, sympathetic activation and endothelial dysfunction are associated with increased cardiovascular risk in patients with chronic kidney disease (CKD). Studies have so far failed to establish the role of vitamin D and vitamin D receptor activator (VDRA) treatment in moderate CKD. This trial was designed to assess whether VDRA treatment can ameliorate sympathetic activation and macro- and microvascular dysfunction in non-diabetic patients with moderate CKD. <b><i>Methods:</i></b> We conducted a randomized controlled double-blind trial using placebo, 1 or 2 μg of paricalcitol, a VDRA, for 3 months. We assessed muscle sympathetic nerve activity (MSNA) by microneurography, pulse wave velocity (PWV) by tonometry, flow mediated vasodilatation (FMD) by brachial ultrasound, skin microcirculation assessed by iontophoresis and capillary blood velocity (CBV) by videophotometric capillaroscopy. <b><i>Results:</i></b> Thirty-six patients with a mean age of 65 years and mean estimated glomerular filtration rate of 40 ml/min/1.73 m<sup>2</sup> were included. We found a significant decline in endothelial function after 3 months, except in the group receiving 2 μg of paricalcitol. The higher dose (2 μg) seemed to attenuate the decline in microvascular endothelial function, assessed by iontophoresis of acetylcholine (p = 0.06 for all groups, p = 0.65 for the 2 μg group) and for FMD (p = 0.006 for all groups, p = 0.54 for the 2 μg group). We found a borderline significance (p = 0.05) for improved CBV in the treated groups. We found no significant changes between treatments in MSNA, PWV or albuminuria. <b><i>Conclusions:</i></b> Endothelial function declined significantly over 3 months in patients with moderate CKD, and this decline could be ameliorated by VDRA treatment (NCT01204528).