DOI: 10.5061/dryad.bf74g
title: Data from: Phylogenetic analyses reveal molecular signatures associated with functional divergence among Subtilisin like Serine Proteases are linked to lifestyle transitions in Hypocreales

description: Background: Subtilisin-like serine proteases or Subtilases in fungi are
 important for penetration and colonization of host. In Hypocreales, these
 proteins share several properties with other fungal, bacterial, plant and
 mammalian homologs. However, adoption of specific roles in
 entomopathogenesis may be governed by attainment of unique biochemical and
 structural features during the evolutionary course. Due to such functional
 shifts Subtilases coded by different family members of Hypocreales acquire
 distinct features according to respective hosts and lifestyle. We
 conducted phylogenetic and DIVERGE analyses and identified important
 protein residues that putatively assign functional specificity to
 Subtilases in fungal families/species under the order Hypocreales.
 Results: A total of 161 Subtilases coded by 10 species from five different
 families under the fungal order Hypocreales was included in the analysis.
 Based on the presence of conserved domains, the Subtilase genes were
 divided into three subfamilies, Subtilisin (S08.005), Proteinase K
 (S08.054) and Serine-carboxyl peptidases (S53.001). These subfamilies were
 investigated for phylogenetic associations, protein residues under
 positive selection and functional divergence among paralogous clades. The
 observations were co-related with the life-styles of the fungal
 families/species. Phylogenetic and Divergence analyses of Subtilisin
 (S08.005) and Proteinase K (S08.054) families of proteins revealed that
 the paralogous clades were clear-cut representation of familial origin of
 the protein sequences. We observed divergence between the paralogous
 clades of plant-pathogenic fungi (Nectriaceae), insect-pathogenic fungi
 (Cordycipitaceae/Clavicipitaceae) and nematophagous fungi
 (Ophiocordycipitaceae). In addition, Subtilase genes from the
 nematode-parasitic fungus Purpureocillium lilacinum made a unique cluster
 which putatively indicated that the fungus might have developed
 distinctive mechanisms for nematode-pathogenesis. Our evolutionary
 genetics analysis revealed evidence of positive selection on the
 Subtilisin (S08.005) and Proteinase K (S08.054) protein sequences of the
 entomopathogenic and nematophagous species belonging to Cordycipitaceae,
 Clavicipitaceae and Ophiocordycipitaceae families of Hypocreales.
 Conclusions: Our study provided new insights into the evolution of
 Subtilisin like serine proteases in Hypocreales, a fungal order largely
 consisting of biological control species. Subtilisin (S08.005) and
 Proteinase K (S08.054) proteins seemed to play important roles during life
 style modifications among different families and species of Hypocreales.
 Protein residues found significant in functional divergence analysis in
 the present study may provide support for protein engineering in future.
description: Protein Sequence of
 Serine Proteases of Purpureocillium_lilacinum_TERI_BC1A total of 44 serine
 protease sequences present in Purpureocillium lilacinum genome are
 provided here.serine_protease.fasta