cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1568972
title: Regional deglaciation and postglacial lake development as reflected in a 74 m sedimentary record from Lake Vättern, southern Sweden

description: The withdrawal of the Late Weichselian ice sheet and rapid isostatic uplift in southern Scandinavia led to the entrainment of large volumes of melt water within the proglacial Baltic Ice Lake (BIL). The eventual western outpost of BIL, Lake Vättern, has been a focal point for studying the dynamic retreat history of the Late Weichselian ice sheet in south central Sweden. This part of the deglacial history is described from an abundance of terrestrial studies, but, to date, no complimentary long sediment cores from Lake Vättern have been available. Here, we present the results from a unique, 74 m borehole in southern Lake Vättern that recovered a Late Pleistocene to Holocene sedimentary sequence. Physical and chemical analyses of the sediment and pore water, together with geophysical mapping, reveal glacial as well as postglacial imprints implying an oscillating ice sheet margin, evidence for neotectonic activity and one or more marine incursions into the lake during deglaciation. We attribute the glaciotectonic deformation of the sediments at 54 m below the lake floor to an ice readvance that likely occurred at the same time or before the advance that formed the Levene moraine (∼13.8–13.4 cal. ka BP). After this event, potential readvances were likely restricted to a more northerly position in the basin. We identify the final drainage of the BIL, but find evidence for an earlier marine incursion into the Vättern basin (∼13.0 cal. ka BP), indicating water exchange between the North Atlantic and the Baltic Ice Lake during the late Alleröd.
DOI: 10.6084/m9.figshare.1568972.v2
title: Regional deglaciation and postglacial lake development as reflected in a 74 m sedimentary record from Lake Vättern, southern Sweden

description: The withdrawal of the Late Weichselian ice sheet and rapid isostatic uplift in southern Scandinavia led to the entrainment of large volumes of melt water within the proglacial Baltic Ice Lake (BIL). The eventual western outpost of BIL, Lake Vättern, has been a focal point for studying the dynamic retreat history of the Late Weichselian ice sheet in south central Sweden. This part of the deglacial history is described from an abundance of terrestrial studies, but, to date, no complimentary long sediment cores from Lake Vättern have been available. Here, we present the results from a unique, 74 m borehole in southern Lake Vättern that recovered a Late Pleistocene to Holocene sedimentary sequence. Physical and chemical analyses of the sediment and pore water, together with geophysical mapping, reveal glacial as well as postglacial imprints implying an oscillating ice sheet margin, evidence for neotectonic activity and one or more marine incursions into the lake during deglaciation. We attribute the glaciotectonic deformation of the sediments at 54 m below the lake floor to an ice readvance that likely occurred at the same time or before the advance that formed the Levene moraine (∼13.8–13.4 cal. ka BP). After this event, potential readvances were likely restricted to a more northerly position in the basin. We identify the final drainage of the BIL, but find evidence for an earlier marine incursion into the Vättern basin (∼13.0 cal. ka BP), indicating water exchange between the North Atlantic and the Baltic Ice Lake during the late Alleröd.
DOI: 10.6084/m9.figshare.1568974.v1
title: Exploring the Association between Working Memory and Driving Performance in Parkinson's disease

description: <i>Objective</i>: The aim of this studywas to explore whether varying levels of operational and tactical driving task demand differentially affect drivers with Parkinson's disease (PD) and control drivers in their sign recall. <i>Methods</i>: Study participants aged between 50 and 70 years included a group of drivers with PD (n = 10) and a group ofage- and sex-matched control drivers(n = 10). Their performance in a sign recall task was measured using a driving simulator. <i>Results</i>: Drivers of the control group performed better than drivers with PD in a sign recall task, but this trend was not statistically significant (p = 0.43).Also, regardless of group membership, subjects' performance differed according to varying levels of task demand. Performance in the sign recall task was more likely to drop with increasing task demand (p = 0.03).This difference was significant when the variation in task demand was associated with a cognitive task, i.e., when drivers were required to apply the instructions from working memory. <i>Conclusions</i>: Although the conclusions drawn from this study are tentative, the evidence presented here is encouraging with regard to the use of a driving simulator to examine isolated cognitive functions underlying driving performance in PD. With an understanding of its limitations, such driving simulation in combination with functional assessment batteries measuring physical, visual and cognitive abilities could comprise one component of a multi-tiered system to evaluate medical fitness to drive.
DOI: 10.6084/m9.figshare.1568969.v2
title: Induction of autophagy by valproic acid enhanced lymphoma cell chemosensitivity through HDAC-independent and IP3-mediated PRKAA activation

description: Autophagy is closely related to tumor cell sensitivity to anticancer drugs. The HDAC (histone deacetylase) inhibitor valproic acid (VPA) interacted synergistically with chemotherapeutic agents to trigger lymphoma cell autophagy, which resulted from activation of AMPK (AMP-activated protein kinase) and inhibition of downstream MTOR (mechanistic target of rapamycin [serine/threonine kinase]) signaling. In an HDAC-independent manner, VPA potentiated the effect of doxorubicin on lymphoma cell autophagy via reduction of cellular inositol 1,4,5 trisphosphate (IP3), blockade of calcium into mitochondria and modulation of PRKAA1/2-MTOR cascade. In murine xenograft models established with subcutaneous injection of lymphoma cells, dual treatment of VPA and doxorubicin initiated IP3-mediated calcium depletion and PRKAA1/2 activation, induced in situ autophagy and efficiently retarded tumor growth. Aberrant genes involving mitochondrial calcium transfer were frequently observed in primary tumors of lymphoma patients. Collectively, these findings suggested an HDAC-independent chemosensitizing activity of VPA and provided an insight into the clinical application of targeting autophagy in the treatment of lymphoma.
DOI: 10.6084/m9.figshare.1568972.v1
title: Regional deglaciation and postglacial lake development as reflected in a 74 m sedimentary record from Lake Vättern, southern Sweden

description: The withdrawal of the Late Weichselian ice sheet and rapid isostatic uplift in southern Scandinavia led to the entrainment of large volumes of melt water within the proglacial Baltic Ice Lake (BIL). The eventual western outpost of BIL, Lake Vättern, has been a focal point for studying the dynamic retreat history of the Late Weichselian ice sheet in south central Sweden. This part of the deglacial history is described from an abundance of terrestrial studies, but, to date, no complimentary long sediment cores from Lake Vättern have been available. Here, we present the results from a unique, 74 m borehole in southern Lake Vättern that recovered a Late Pleistocene to Holocene sedimentary sequence. Physical and chemical analyses of the sediment and pore water, together with geophysical mapping, reveal glacial as well as postglacial imprints implying an oscillating ice sheet margin, evidence for neotectonic activity and one or more marine incursions into the lake during deglaciation. We attribute the glaciotectonic deformation of the sediments at 54 m below the lake floor to an ice readvance that likely occurred at the same time or before the advance that formed the Levene moraine (∼13.8–13.4 cal. ka BP). After this event, potential readvances were likely restricted to a more northerly position in the basin. We identify the final drainage of the BIL, but find evidence for an earlier marine incursion into the Vättern basin (∼13.0 cal. ka BP), indicating water exchange between the North Atlantic and the Baltic Ice Lake during the late Alleröd.
DOI: 10.6084/m9.figshare.1568969.v1
title: Induction of autophagy by valproic acid enhanced lymphoma cell chemosensitivity through HDAC-independent and IP3-mediated PRKAA activation

description: Autophagy is closely related to tumor cell sensitivity to anticancer drugs. The HDAC (histone deacetylase) inhibitor valproic acid (VPA) interacted synergistically with chemotherapeutic agents to trigger lymphoma cell autophagy, which resulted from activation of AMPK (AMP-activated protein kinase) and inhibition of downstream MTOR (mechanistic target of rapamycin [serine/threonine kinase]) signaling. In an HDAC-independent manner, VPA potentiated the effect of doxorubicin on lymphoma cell autophagy via reduction of cellular inositol 1,4,5 trisphosphate (IP3), blockade of calcium into mitochondria and modulation of PRKAA1/2-MTOR cascade. In murine xenograft models established with subcutaneous injection of lymphoma cells, dual treatment of VPA and doxorubicin initiated IP3-mediated calcium depletion and PRKAA1/2 activation, induced in situ autophagy and efficiently retarded tumor growth. Aberrant genes involving mitochondrial calcium transfer were frequently observed in primary tumors of lymphoma patients. Collectively, these findings suggested an HDAC-independent chemosensitizing activity of VPA and provided an insight into the clinical application of targeting autophagy in the treatment of lymphoma.
DOI: 10.6084/m9.figshare.1568950
title: Genotoxic and cytotoxic effects and gene expression changes induced by fixed orthodontic appliances in oral mucosa cells of patients: a systematic review

description: <i>Context</i>: The accumulation of chronic or severe acute DNA and cellular damage in oral mucosa cells is one of the main factors that help initiate a wide range of malignant lesions in the oral cavity. There has been considerable controversy in the literature about the effect of such sustained genotoxic and cytotoxic damage to oral mucosa cells.<i>Objective</i>: The aim of this systematic review, reported in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, is to investigate the effects of such interventions.<i>Methods</i>: Electronic and manual searches were performed (15 May 2015) for Randomized Clinical Trials/quasi-Randomized Clinical Trials that analyzed the genotoxic/cytotoxic effects of these types of oral appliances in humans. A primary outcome (cell/DNA damage) and a number of secondary outcomes were examined. Two reviewers carried out the study selection and performed a “risk of bias” assessment [Cochrane Collaboration's tool]. Wherever possible the meta-analysis was conducted on homogenous groups.<i>Results</i>: From the electronic search (2797), 6 studies met the eligibility criteria. Most studies (5/6) observed significant differences in most comparisons at the short-term (1-3 months) and long-term (24–48 months) evaluations, with respect to critically acute genotoxic/cytotoxic effects. Some of the studies (2/3) concluded that the post-removable effects at DNA/cellular levels were not significant (<i>p</i> &gt; 0.05) with respect to the controls.<i>Conclusions</i>: Acute DNA/cellular damage in oral mucosa cells is induced by orthodontic appliances. Nevertheless, even though these effects were no longer detected after removing the appliances, more rigorous RCTs are needed to explore the extent to which acquired damage can be observed in the oral mucosa.
DOI: 10.6084/m9.figshare.1568950.v1
title: Genotoxic and cytotoxic effects and gene expression changes induced by fixed orthodontic appliances in oral mucosa cells of patients: a systematic review

description: <i>Context</i>: The accumulation of chronic or severe acute DNA and cellular damage in oral mucosa cells is one of the main factors that help initiate a wide range of malignant lesions in the oral cavity. There has been considerable controversy in the literature about the effect of such sustained genotoxic and cytotoxic damage to oral mucosa cells.<i>Objective</i>: The aim of this systematic review, reported in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, is to investigate the effects of such interventions.<i>Methods</i>: Electronic and manual searches were performed (15 May 2015) for Randomized Clinical Trials/quasi-Randomized Clinical Trials that analyzed the genotoxic/cytotoxic effects of these types of oral appliances in humans. A primary outcome (cell/DNA damage) and a number of secondary outcomes were examined. Two reviewers carried out the study selection and performed a “risk of bias” assessment [Cochrane Collaboration's tool]. Wherever possible the meta-analysis was conducted on homogenous groups.<i>Results</i>: From the electronic search (2797), 6 studies met the eligibility criteria. Most studies (5/6) observed significant differences in most comparisons at the short-term (1-3 months) and long-term (24–48 months) evaluations, with respect to critically acute genotoxic/cytotoxic effects. Some of the studies (2/3) concluded that the post-removable effects at DNA/cellular levels were not significant (<i>p</i> &gt; 0.05) with respect to the controls.<i>Conclusions</i>: Acute DNA/cellular damage in oral mucosa cells is induced by orthodontic appliances. Nevertheless, even though these effects were no longer detected after removing the appliances, more rigorous RCTs are needed to explore the extent to which acquired damage can be observed in the oral mucosa.
DOI: 10.6084/m9.figshare.1568948.v1
title: Variable presence of 5-methylcytosine in commercial RNA and DNA

description: 5-methylcytosine (m<sup>5</sup>C, mC) is a naturally occurring nucleoside modification in both RNA and DNA. Its presence in DNA is a widely accepted epigenetic mark for transcription inactivation. In RNA, its appearance in different coding as well as non-coding RNA implies multiple functions, with regulation of gene expression as a common denominator. Here we report on the serendipitous discovery of m<sup>5</sup>C in synthetic oligonucleotides, which prompted a systematic quantification in synthetic DNA and RNA of academic as well as of commercial origin. For both types of oligonucleotides, m<sup>5</sup>C was identified by comparison of fragmentation pattern and retention time with authentic standards by highly sensitive LC-MS/MS analysis with limit-of-quantification values in the attomol range. Quantification revealed m<sup>5</sup>C contents ranging from the ppm range to single digit percentiles for certain commercial suppliers, while others remained below the single digit promille mark. The only oligonucleotides without m<sup>5</sup>C were entirely devoid of cytosines in their sequence, thus tying m<sup>5</sup>C occurance to the use of cytosine phosphoramidites during synthesis. These findings may have important bearings for the use of synthetic oligonucleotides under such diverse aspects as gene expression, immunostimulation, or biophysical studies of duplex stability.
DOI: 10.6084/m9.figshare.1568948.v2
title: Variable presence of 5-methylcytosine in commercial RNA and DNA

description: Variable presence of 5-methylcytosine in commercial RNA and DNA
DOI: 10.6084/m9.figshare.1568936
title: Assessment of testicular dose during preoperative radiotherapy for rectal cancer

description: <i>Background</i>: Radiotherapy (RT) for rectal cancer can have adverse effects on testicular function resulting in azoospermia and low testosterone levels. Variability of testicular dose (TD) due to differences in position of testes has been assessed with scrotal dosimeters and resulted in substantial variability of delivered TD. The aim of this study was to estimate planned and delivered TD using a treatment planning system (TPS). <i>Methods</i>: In 101 men treated with RT for rectal cancer the cumulative mean TD (mTD) was calculated by TPS based on plan-computed tomography (CT) to evaluate the effect of different predictors on planned TD. The delivered TD was estimated by TPS based on repeated cone-beam CTs in 32 of 101 men to assess within-person variability of planned and delivered TD in a longitudinal analysis. <i>Results</i>: The median planned mTD for short course RT was 0.57 Gy (range 0.06–14.37 Gy) and 0.81 Gy (range 0.36–10.80 Gy) for long course RT. The median planned mTD was similar to the median delivered mTD in the 32 men analysed over the entire course of RT (p=0.84). The mTD did not change significantly over time of planning and delivering RT. The variation in proximity between testes and planning target volume (PTV) was related to within-person variability of mTD in men on the 50th and 75th percentile of mTD and as expected the absolute difference between planned and delivered mTD increased with higher mTD. <i>Conclusion</i>: Testicular doses calculated based on plan-CT are an accurate estimation of delivered TD based on repeated cone beam (CB)CT. The within-person variability of TD is related to variation in proximity between testes and PTV in men with moderate to high TD.
DOI: 10.6084/m9.figshare.1568948.v3
title: Variable presence of 5-methylcytosine in commercial RNA and DNA

description: Variable presence of 5-methylcytosine in commercial RNA and DNA
DOI: 10.6084/m9.figshare.1568936.v2
title: Assessment of testicular dose during preoperative radiotherapy for rectal cancer

description: <i>Background</i>: Radiotherapy (RT) for rectal cancer can have adverse effects on testicular function resulting in azoospermia and low testosterone levels. Variability of testicular dose (TD) due to differences in position of testes has been assessed with scrotal dosimeters and resulted in substantial variability of delivered TD. The aim of this study was to estimate planned and delivered TD using a treatment planning system (TPS). <i>Methods</i>: In 101 men treated with RT for rectal cancer the cumulative mean TD (mTD) was calculated by TPS based on plan-computed tomography (CT) to evaluate the effect of different predictors on planned TD. The delivered TD was estimated by TPS based on repeated cone-beam CTs in 32 of 101 men to assess within-person variability of planned and delivered TD in a longitudinal analysis. <i>Results</i>: The median planned mTD for short course RT was 0.57 Gy (range 0.06–14.37 Gy) and 0.81 Gy (range 0.36–10.80 Gy) for long course RT. The median planned mTD was similar to the median delivered mTD in the 32 men analysed over the entire course of RT (p=0.84). The mTD did not change significantly over time of planning and delivering RT. The variation in proximity between testes and planning target volume (PTV) was related to within-person variability of mTD in men on the 50th and 75th percentile of mTD and as expected the absolute difference between planned and delivered mTD increased with higher mTD. <i>Conclusion</i>: Testicular doses calculated based on plan-CT are an accurate estimation of delivered TD based on repeated cone beam (CB)CT. The within-person variability of TD is related to variation in proximity between testes and PTV in men with moderate to high TD.
DOI: 10.6084/m9.figshare.1568936.v1
title: Assessment of testicular dose during preoperative radiotherapy for rectal cancer

description: <i>Background</i>: Radiotherapy (RT) for rectal cancer can have adverse effects on testicular function resulting in azoospermia and low testosterone levels. Variability of testicular dose (TD) due to differences in position of testes has been assessed with scrotal dosimeters and resulted in substantial variability of delivered TD. The aim of this study was to estimate planned and delivered TD using a treatment planning system (TPS).<i>Methods</i>: In 101 men treated with RT for rectal cancer the cumulative mean TD (mTD) was calculated by TPS based on plan-computed tomography (CT) to evaluate the effect of different predictors on planned TD. The delivered TD was estimated by TPS based on repeated cone-beam CTs in 32 of 101 men to assess within-person variability of planned and delivered TD in a longitudinal analysis.<i>Results</i>: The median planned mTD for short course RT was 0.57 Gy (range 0.06–14.37 Gy) and 0.81 Gy (range 0.36–10.80 Gy) for long course RT. The median planned mTD was similar to the median delivered mTD in the 32 men analysed over the entire course of RT (p=0.84). The mTD did not change significantly over time of planning and delivering RT. The variation in proximity between testes and planning target volume (PTV) was related to within-person variability of mTD in men on the 50th and 75th percentile of mTD and as expected the absolute difference between planned and delivered mTD increased with higher mTD.<i>Conclusion</i>: Testicular doses calculated based on plan-CT are an accurate estimation of delivered TD based on repeated cone beam (CB)CT. The within-person variability of TD is related to variation in proximity between testes and PTV in men with moderate to high TD.
DOI: 10.6084/m9.figshare.1568948
title: Variable presence of 5-methylcytosine in commercial RNA and DNA

description: Variable presence of 5-methylcytosine in commercial RNA and DNA
DOI: 10.6084/m9.figshare.1568948.v5
title: Variable presence of 5-methylcytosine in commercial RNA and DNA

description: Variable presence of 5-methylcytosine in commercial RNA and DNA
DOI: 10.6084/m9.figshare.1568948.v4
title: Variable presence of 5-methylcytosine in commercial RNA and DNA

description: Variable presence of 5-methylcytosine in commercial RNA and DNA