cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0xp750f
title: CIL:36183, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (polyclonal Ab266, S. Halpain, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]) and tubulin (DM1A, Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]). Images were acquired with a Leica DMRA microscope with a 20X lens (Fluotar, NA 0.5), Photometrics CoolSnap ES CCD camera and MetaMorph software. This color was generated using the MetaMorph "color combine" function.
description: Development of cytoskeletal polarity in cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (red) and tubulin, which is present in both axons and dendrites (green).
DOI: 10.6075/j00r9nvh
title: CIL: 51789, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library

description: A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding drug slowly in 1/4 increments over 90 minutes.
description: Imaging mode: DICM
Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective
description: ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C

Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files:
1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html)
2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/)
3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory
4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK"
5. The 16 bit image will typically look dark and will require brightness adjustment.
6. In the ImageJ toolbar, select Image &gt;&gt; Adjust &gt;&gt; Brightness/Contrast and select "Auto", or manually set the brightness and contrast.
DOI: 10.6075/j070811h
title: CIL: 52866, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: Tile x=4, y=5, z=1 from PS11_167511L_Myc_Terc
description: One z layer of a z-stack at the given tile position
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
DOI: 10.6075/j0mk6bvh
title: CIL:25403, Drosophila melanogaster, fat body cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; acetic acid treatment
Relation to intact cell: squashed preparation
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Whole mount squash of Drosophila polytene chromosomes viewed with high voltage EM showing a series of bands and interbands. This micrograph was recorded with a tilt angle of 46 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.
description: Fat body cells from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid containing 0.1% Triton-X 100, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated and transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film, are transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.
DOI: 10.6075/j0862g3w
title: CIL: 53863, Homo sapiens, HeLa. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: OTHER family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Visualization methods: confocal microscopy
DOI: 10.6075/j0959h9m
title: CIL: 54574, Xenopus Laevis, Entire Organism. In Cell Image Library

description: Video of African clawed frog embryonic development. Video filename format is as follows organism_temperature_batch. Data was collected at 16.4 degrees Celcius
description: Imaging mode: RGB
Visualization methods: Captured on a microscope using a Canon Rebel Ti5/6 or a one of the following eyepiece cameras: Swiftcam 3 Megapixel, OMAX 9.0 MP, AmScope MU300
description: 16.4 degrees C
DOI: 10.15468/dl.7zgfhu
title: Occurrence Download

description: A dataset containing 2284 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Festuca elegans"
 ]
}

 The dataset includes 2284 records from 80 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009488-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j00z72h9
title: CIL:2236, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 6 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j0m9089g
title: CIL: 53505. In Cell Image Library

description: AGC family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Visualization methods: confocal microscopy
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
DOI: 10.6075/j0tq60bw
title: CIL:11043, Notophthalmus viridescens, oocyte. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Micrograph originally published in Miller & Beatty, Science 164:955-957, and reprinted with permission as Figure 144 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Transmission electron micrograph of transcription of tandemly arranged ribosomal RNA genes. Amphibian oocyte nuclei contain numerous extra-chromosomal nucleoli, each of which contains a tandem array of genes encoding ribosomal RNA. Here, using the methods developed by O.L. Miller Jr. and colleagues, the extrachromosomal nucleoli from a newt oocyte have been dispersed in low salt, and centrifuged through a sucrose cushion containing formaldehyde on to a carbon coated grid and stained for TEM imaging. Active transcription is seen by the tree-like structures in which each branch represents a growing transcript. Untranscribed spacer DNA is seen between each transcript.
DOI: 10.6075/j02n510x
title: CIL:12071, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: This high resolution EM features the striated bands that link the membranes under the surface depressions of the cortex. The bands insert into the epiplasm, and in this case, they separate to pass around the parasomal sacs which lie to the right of a row of basal body/cilium complexes. The bands have relatively electron transparent zones next to the epiplasm that lie next to more opaque zones which are in turn separated by a central medium electron transparent zone. This differential banding suggests an overlap of filaments in the two more opaque zones, but there is no evidence of a muscle-like actomyosin system. These bands probably provide tension and keep the cortical ridges from flattening. If contractile they could account for the cell's ability to alter its shape when swimming through constricted regions. For details on the striated bands in Paramecium see Allen, J. Cell Biol. 49:1-20, 1971 and Allen, Aihara and Fok, J. Euk . Microbiol. 45:202-209, 1998. This image is adapted with permission from Fig. 14 in J. Cell Biol. 49:1-20, 1971. TEM taken on 12/18/68 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.5291/ill-data.3-14-438
title: UCN storage in small cryogenic cells
DOI: 10.5291/ill-data.5-31-3131
title: Unusual effect of crystal and magnetic structure transformation in the multi-valent La0.5Ba0.5CoO2.85 irradiated by pulsed electrons & ions
DOI: 10.5291/ill-data.5-31-2951
title: Ultra High Resolution SANS Study of the IMS Domain Morphology with Transport Current
DOI: 10.15468/dl.cj35r4
title: Occurrence Download

description: A dataset containing 359 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Spain",
 "TaxonKey is Apium repens"
 ]
}

 The dataset includes 359 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009489-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5291/ill-data.5-31-2911
title: Continuation:  Self Organisation of the IMS under the Application of a Current
DOI: 10.5291/ill-data.5-31-2748
title: Self Organisation of Mixed Superconducting States under the Application of a Current
DOI: 10.5291/ill-data.5-31-2750
title: Investigating Radial Dependence of  Field and Current Distribution in a Superconducting Niobium Wire
DOI: 10.5291/ill-data.5-41-1325
title: Antiferromagnetic origin of giant anomalous Hall conductivity in TaMnP
DOI: 10.5291/ill-data.5-42-579
title: Stop-and-go Kinetic Neutron Scattering of Current-driven Vortex Clusters
DOI: 10.5291/ill-data.5-42-573
title: Unveiling the Self-organisation Mechanism of the Intermediate Mixed State
DOI: 10.5291/ill-data.5-61-46
title: Investigation of Meta Vortex Physics in a superconducting Corbino disk
DOI: 10.15468/dl.kcztaj
title: Occurrence Download

description: A dataset containing 247 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Spain",
 "TaxonKey is Nuphar lutea"
 ]
}

 The dataset includes 247 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009490-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5291/ill-data.9-11-2271
title: Probing water dynamics in organic PANI-based cathode materials
DOI: 10.5291/ill-data.dir-284
title: SEARCH FOR THE VORTEX LATTICE IN A ROOM TEMPERATURE SUPERCONDUCTOR
DOI: 10.5291/ill-data.easy-568
title: The effects of current on the intermediate mixed stat in niobium
DOI: 10.15468/dl.q4skgc
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is CHN.28_1",
 "TaxonKey is Erigeron schmalhausenii"
 ]
}

 The dataset includes 21 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009492-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vyumyg
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is CHN.28_1",
 "TaxonKey is Achillea alpina"
 ]
}

 The dataset includes 2 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009493-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.e3rzdz
title: Occurrence Download

description: A dataset containing 36515 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Taxon occurrence data for the FungalRoot database"
 ]
}

 The dataset includes 36515 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009491-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9zakk3
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is CHN.28_1",
 "TaxonKey is Neottia listeroides"
 ]
}

 The dataset includes 3 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009494-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/13564173
title: Bangladeshi Teachers Dataset
DOI: 10.15468/dl.ythsrq
title: Occurrence Download

description: A dataset containing 793 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Pinus bungeana"
 ]
}

 The dataset includes 793 records from 121 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009496-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dsd62x
title: Occurrence Download

description: A dataset containing 87543 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Observation, Machine Observation, Human Observation, Occurrence evidence)",
 "DatasetKey is one of (iNaturalist Research-grade Observations, 2021_nsmk_biodiversity_observations_bi_eagle_113068, 2021_nsmk_biodiversity_observations_bird_197960, 2021_nsmk_biodiversity_observations_bi_eagle_43792, Taiwan New Year Bird Count, The Taiwan Breeding Bird Survey Data, Observation.org, Nature data from around the World, 2021_nsmk_biodiversity_observations_bi_eagle_81252, 2021_nsmk_biodiversity_observations_bird_136784, 2020_biodiversity_information_observation, 2021_nsmk_biodiversity_observations_bi_eagle_12000, 2021_nsmk_biodiversity_observations_bird_8611, Occurrence dataset of birds in the Sihong Hongze Lake Wetland National Nature Reserve,Jiangsu, China, NATURING_CS_Observation_2020, 成龍溼地鳥類生態紀錄)",
 "Geometry POLYGON((136.2409 48.25909,136.0935 49.71547,129.7755 51.96353,123.8523 56.6137,117.5179 53.38808,109.0445 44.53851,104.2731 44.1065,97.69184 45.1487,92.859 47.24349,89.43675 50.39491,86.56293 50.62786,82.17543 48.39635,81.25405 46.78444,78.95061 46.23386,78.29248 42.9243,73.30169 41.62048,72.22675 39.56453,72.86843 36.80013,76.83638 34.02585,77.64807 30.41091,85.57848 26.89501,89.11134 26.22055,90.57841 26.78684,91.53818 25.82537,93.92389 25.82537,95.98053 26.91048,96.61124 26.42441,95.73373 22.24479,101.9311 19.76072,103.5764 21.21963,105.0572 21.44189,107.7555 20.30733,106.8149 18.09667,109.8999 16.73974,119.429 22.45741,134.5339 44.40329,136.2409 48.25909))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Aves",
 "Year is 2020"
 ]
}

 The dataset includes 87543 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009482-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.30498881
title: Supplementary Material for: Cutaneous Metastasis from Renal Cell Carcinoma (RCC): A Case Report

description: Introduction: 
Cutaneous metastases from RCC is an uncommon and poorly understood clinical phenomenon that frequently predicts a poor prognosis. Case presentation: A 69-year-old male with a history of type 2 diabetes and benign prostatic hyperplasia (BPH) was diagnosed with stage 4 renal cell carcinoma (RCC) and various cutaneous lesions. A dermatological checkup during his hospitalization revealed asymptomatic subcutaneous nodules and purpuric areas. Biopsy and immunohistochemistry (IHC) staining confirmed the presence of cutaneous metastatic carcinoma, which is consistent with renal cell origin. The immunohistochemistry profile was positive for pancytokeratin, CD10, and vimentin but negative for PAX8, indicating a distinct appearance in RCC patients. 
Conclusions: This case emphasizes the necessity of recognizing cutaneous metastases as an uncommon presentation of advanced RCC, as well as PAX8's potential role as a diagnostic marker.
DOI: 10.6084/m9.figshare.30498881.v1
title: Supplementary Material for: Cutaneous Metastasis from Renal Cell Carcinoma (RCC): A Case Report

description: Introduction: 
Cutaneous metastases from RCC is an uncommon and poorly understood clinical phenomenon that frequently predicts a poor prognosis. Case presentation: A 69-year-old male with a history of type 2 diabetes and benign prostatic hyperplasia (BPH) was diagnosed with stage 4 renal cell carcinoma (RCC) and various cutaneous lesions. A dermatological checkup during his hospitalization revealed asymptomatic subcutaneous nodules and purpuric areas. Biopsy and immunohistochemistry (IHC) staining confirmed the presence of cutaneous metastatic carcinoma, which is consistent with renal cell origin. The immunohistochemistry profile was positive for pancytokeratin, CD10, and vimentin but negative for PAX8, indicating a distinct appearance in RCC patients. 
Conclusions: This case emphasizes the necessity of recognizing cutaneous metastases as an uncommon presentation of advanced RCC, as well as PAX8's potential role as a diagnostic marker.