cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/8x2fq-3zp88
title: pED208_6um

description: t4ss in E.colli with F pilus
description: <strong>Tilt Series Date:</strong> 2015-02-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> LB o/n 37C</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-02-11-50/rawdata/</p>pED-6um-20016.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-02-11-50/rawdata/pED-6um-20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED-6um-20016_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-02-11-50/3dimage_51805/pED-6um-20016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2gd6t-mxs63
title: nanochemowt_2ndday

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-2/rawdata/</p>nanochemowt2day_atlas10002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-2/rawdata/nanochemowt2day_atlas10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        nanochemowt2day_atlas10002_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-2/3dimage_58079/nanochemowt2day_atlas10002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vh9pd-3xz86
title: Mycobacterium marinum

description: Grown on 2x LB to induce sporulation
description: <strong>Tilt Series Date:</strong> 2015-10-12</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Mycobacterium marinum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Will DePas from Dianne Newman's lab grew the cells</p>
<strong>Sample Preparation:</strong> Cells were frozen with Vitrobot Mark 4. Grids were positively glow discharged.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-12-14/3dimage_70136/</p>Mmarinum_oct20150014_part121_6.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-12-14/3dimage_70136/Mmarinum_oct20150014_part121_6.rec"
        > <i class="download icon"></i> Download </a></p>

        Mmarinum_oct20150014.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-12-14/rawdata/Mmarinum_oct20150014.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1z0v8-4rx80
title: stable HAMP mutant

description: UU2612 with stable HAMP
description: <strong>Tilt Series Date:</strong> 2015-03-12</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sandy Parkinson University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB plus gent, Amp, 50 µl 10mM IPTG overnight, penicillin lysis</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-8/rawdata/</p>stableHAMPgrid20001.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-8/rawdata/stableHAMPgrid20001.mrc"
        > <i class="download icon"></i> Download </a></p>

        stableHAMPgrid20001_part121_46.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-8/3dimage_53204/stableHAMPgrid20001_part121_46.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nth1f-8pa38
title: Crosslinked HIV-1 cores TRIM5α

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5α
description: <strong>Tilt Series Date:</strong> 2015-01-03</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 109.0 eV/Å²,  defocus: -5.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores decorated with TRIM5α. 0.3 µM sulfo-EGS</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-9/rawdata/</p>tomography0010.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-9/rawdata/tomography0010.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography0010_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-9/3dimage_50116/tomography0010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hfe9f-fka84
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-348/rawdata/</p>T4SSWTNov26A055_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-348/rawdata/T4SSWTNov26A055_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov26A055_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-348/3dimage_105095/T4SSWTNov26A055_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/16eqw-kkd46
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-90/rawdata/</p>LP02May18B026_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-90/rawdata/LP02May18B026_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        LP02May18B026_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-90/3dimage_104889/LP02May18B026_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gms97-kjt45
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-431/rawdata/</p>T4SSWTminiNov18C019_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-431/rawdata/T4SSWTminiNov18C019_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTminiNov18C019_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-431/3dimage_105160/T4SSWTminiNov18C019_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3a8g1-hsn12
title: TM7 tomogram data

description: TM7 human oral cavity
description: <strong>Tilt Series Date:</strong> 2017-05-05</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Candidatus Saccharibacteria</p>
<strong>Strain:</strong> TM7</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-53.0°, 53.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Wenyuan Shi</p>
<strong>Sample Preparation:</strong> Force 6/ time 4/ duration 2 2 ul loading with 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-05-35/3dimage_84265/</p>8tgt_2.rec,  Reconstruction (Pixel Size 1.92 nm), 155.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-05-35/3dimage_84265/8tgt_2.rec"
        > <i class="download icon"></i> Download </a></p>

        8tgt_2.mrc,  Tilt Series (Pixel Size 0.48 nm), 761.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-05-35/rawdata/8tgt_2.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ak4d7-vw480
title: stable HAMP mutant

description: UU2612 with stable HAMP
description: <strong>Tilt Series Date:</strong> 2015-03-12</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sandy Parkinson University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB plus gent, Amp, 50 µl 10mM IPTG overnight, penicillin lysis</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-12/rawdata/</p>stableHAMPgrid20005.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-12/rawdata/stableHAMPgrid20005.mrc"
        > <i class="download icon"></i> Download </a></p>

        stableHAMPgrid20005_part121_29.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-12-12/3dimage_53212/stableHAMPgrid20005_part121_29.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r9tj6-sjw16
title: nanochemowt_2ndday

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-61/rawdata/</p>nanochemowt2day_atlas30021.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-61/rawdata/nanochemowt2day_atlas30021.mrc"
        > <i class="download icon"></i> Download </a></p>

        nanochemowt2day_atlas30021_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-61/3dimage_58189/nanochemowt2day_atlas30021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qfg73-nkv17
title: Vibrio cholerae

description: Raw data files of Vibrio cholerae
description: <strong>Tilt Series Date:</strong> 2015-10-01</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> PM22, lacZ- Δvc1397 Δvc1402</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Simon Ringgaard</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Briegel, A., Ortega, D. R., Mann, P., Kjaer, A., Ringgaard, S. and Jensen, G. J. (2016). Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM. Proc Natl Acad Sci U S A 113, 10412-10417.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-12/3dimage_69754/</p>PM22deltaCheA1trippleW0012_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-12/3dimage_69754/PM22deltaCheA1trippleW0012_full.rec"
        > <i class="download icon"></i> Download </a></p>

        PM22deltaCheA1trippleW0012.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-12/rawdata/PM22deltaCheA1trippleW0012.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/st9dr-v2913
title: nanochemowt

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-02</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 16h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-02-5/rawdata/</p>nanochemo_atlas10016.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-02-5/rawdata/nanochemo_atlas10016.mrc"
        > <i class="download icon"></i> Download </a></p>

        nanochemo_atlas10016.rec,  Reconstruction (Pixel Size 1.92 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-02-5/3dimage_57753/nanochemo_atlas10016.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rf0t3-c0453
title: Cos7 cells

description: Synchronized Cos7 cells, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2015-01-20</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Cos7</p>
<strong>Strain:</strong> Cos7 cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Sample Preparation:</strong> Synchronized Cos7 cells with siRNA NUP 153 and Thymidine 2mM</p>
  rra2015-01-20-7_slicer12705.jpg: Microtubules and multilamelar body .  [2015-02-17 12:43:30] Autophagosome rra2015-01-20-7_slicer12717.jpg:  .  [2015-02-17 12:43:05] Autophagosome
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-20-7/rawdata/</p>cos10002.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-20-7/rawdata/cos10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        cos10002_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-20-7/3dimage_51139/cos10002_full.rec"
        > <i class="download icon"></i> Download </a></p>