DOI: 10.5061/dryad.9133
title: Data from: Adaptive landscapes in evolving populations of Pseudomonas fluorescens

description: The repeatability of adaptive evolution depends on the ruggedness of the
 underlying adaptive landscape. We contrasted the relative ruggedness of
 adaptive landscapes across two environments by measuring the variance in
 fitness and metabolic phenotype within and among genetically distinct
 strains of Pseudomonas fluorescens in two environments differing only in
 the carbon source provided (glucose vs. xylose). Fitness increased in all
 lines, plateauing in one environment but not the other. The pattern of
 variance in fitness among replicate lines was unique to the selection
 environment; it increased over the course of the experiment in xylose but
 not in glucose. Metabolic phenotypes displayed two results: (1)
 populations adapted via changes that were distinctive to their selection
 environment, and (2) endpoint phenotypes were less variable in glucose
 than in xylose. These results indicate that although the response to
 selection is highly repeatable at the level of fitness, the underlying
 genetic routes taken were different for each environment and more variable
 in xylose. We suggest that this reflects a more rugged adaptive landscape
 in xylose compared to glucose. Our study demonstrates the utility of using
 replicate selection lines with different evolutionary starting points to
 try and quantify the relative ruggedness of adaptive landscapes.
description: BOTHRelWRelative fitness values collected in the lab via competition between evolved and ancestral strain of P. fluorescens. Column headings are: SelE=selection environment, Geno=starting genotype, replicate=replicate population number, Relw=relative fitnessGsRinitialThis data file are the initial OD readings from the BIOLOG plates of glucose evolved populations. It was used to form the adjusted BIOLOG data by subtracting it from the values in "GsRfinal.csv". Column headings are time=generation; rep=replicate population; geno=original starting genotype; A1 through H12 correspond to wells on the 96 well BIOLOG plate (see BIOLOG for carbon substrates).GsRfinalThis data file are the final OD readings from the BIOLOG plates of glucose evolved populations. It was used to form the adjusted BIOLOG data by subtracting from the values in "GsRinitial.csv". Column headings are time=generation; rep=replicate population; geno=original starting genotype; A1 through H12 correspond to wells on the 96 well BIOLOG plate (see BIOLOG for carbon substrates).XsRinitialThis data file are the initial OD readings from the BIOLOG plates of xylose evolved populations. It was used to form the adjusted BIOLOG data by subtracting it from the values in "XsRfinal.csv". Column headings are time=generation; rep=replicate population; geno=original starting genotype; A1 through H12 correspond to wells on the 96 well BIOLOG plate (see BIOLOG for carbon substrates).XsRfinalThis data file are the final OD readings from the BIOLOG plates of xylose evolved populations. It was used to form the adjusted BIOLOG data by subtracting it from the values in "XsRinitial.csv". Column headings are time=generation; rep=replicate population; geno=original starting genotype; A1 through H12 correspond to wells on the 96 well BIOLOG plate (see BIOLOG for carbon substrates).