cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.26008/1912/bco-dmo.929459.1
title: Laboratory-cultured Synechococcus (WH8102 and WH5701) MUF-P hydrolysis inhibition by dissolved organic phosphorus (DOP) from experiments between 2018-2023

description: The affinity of Synechococcus (WH8102 and WH5701) alkaline phosphatases for different DOP model substrates was examined in laboratory experiments through its ability to inhibit the hydrolysis of the fluorogenic substrate 4-methylumbelliferyl phosphate (MUF-P). These data were collected as part of a study of &quot;Dissolved organic phosphorus bond-class utilization by Synechococcus&quot; (Waggoner et al., 2024).
 
Study Abstract:
Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
DOI: 10.26008/1912/bco-dmo.929471.1
title: In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023

description: Marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory culture in vivo fluorescence and flow cytometry cell counts following growth in high and low phosphate media, as part of the DOP hydrolysis experiments in a study of &quot;Dissolved organic phosphorus bond-class utilization by Synechococcus&quot; (Waggoner et al. 2024). 

Study Abstract:
Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
DOI: 10.26008/1912/bco-dmo.928984.1
title: Hydrolysis rates from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023

description: Dissolved organic phosphorus (DOP) hydrolysis rates from marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory cultures.  These data were collected as part of a study of &quot;Dissolved organic phosphorus bond-class utilization by Synechococcus&quot; (Waggoner et al., 2024).

Study Abstract:
Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
DOI: 10.26008/1912/bco-dmo.929212.1
title: Laboratory-cultured Synechococcus (WH8102 and WH5701) growth (vivo fluorescence) on dissolved organic phosphorus (DOP) from experiments between 2018-2023

description: Laboratory culture Synechococcus (WH8102 and WH5701) growth on dissolved organic phosphorus (DOP). These data were collected as part of a study of &quot;Dissolved organic Phosphorus bond-class utilization by Synechococcus&quot; (Waggoner et al. 2024). 

Study Abstract:
Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
DOI: 10.26008/1912/bco-dmo.907880.1
title: Post-diapause Neocalanus flemingeri females morphometric measurements and calculations of lipid fullness and lipid volume taken from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13

description: Morphometric data on Neocalanus flemingeri females were made on individuals collected in diapause in June and September, 2019 and after incubation in the laboratory for up to 4.5 weeks. Collections were made from depth in Prince William Sound in the Gulf of Alaska during two NGA LTER cruises. After sorting, females were incubated in flasks at 4-6ºC for up to 4.5 weeks. Individuals were removed for imaging and experimental incubations to study oocyte production. Morphometric analysis was used to measure prosome length and area as well as lipid sac area for lipid fullness and lipid volume calculations. Lipid consumption during diapause was minimal and modest initially.
DOI: 10.26008/1912/bco-dmo.908514.1
title: Oocytes formation in post-diapause Neocalanus flemingeri females from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13

description: These data are from a study on the formation of oocytes in post-diapause Neocalanus flemingeri females collected from depth in Prince William Sound in the Gulf of Alaska. Collections were made during two NGA LTER cruises. After sorting, females were incubated in flasks and removed for experimental incubations and imaging. Oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2’-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU labeled cells peaking at 72 hours following diapause termination. Cells labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. By limiting DNA replication to the initial phase, the females effectively separate oocyte production from oocyte provisioning.
DOI: 10.26008/1912/bco-dmo.928039.1
title: Bacterial transcriptional response to picoeukaryote Micromonas commoda

description: Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. However, direct influences of bacteria on phytoplankton physiology are poorly known. In this study, three marine bacteria (Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, and Polaribacter dokdonensis MED152) were co-cultured with green alga Micromonas commoda, and the phytoplankter's transcriptome was studied by RNASeq. The presence of each bacterium invoked transcriptomic remodeling by M. commoda after 8 h in co-culture. Some aspects of the algal transcriptomic response were conserved across all three bacteria, while others were restricted to a single bacterium. M. commoda had both rapid and extensive responses to heterotrophic bacteria.
DOI: 10.26008/1912/bco-dmo.880760.2
title: Sample stations for the Neotrypaea COP (Community, Oxygen, & Productivity) Effects ground-truth cruises in 2021 and 2022

description: Benthic invertebrate communities, that the highly productive US West Coast fishery species and marine mammals rely on for food, are being increasingly impacted by low oxygen conditions. This project addresses the potential ecological consequences of a new member to these benthic communities, the ghost shrimp Neotrypaea. In estuaries, Neotrypaea continuously rework the sediment via their burrowing activities. Their high abundances and geological effects make them important in mitigating impacts of nutrient run-off (natural and human-induced), which can exacerbate low oxygen conditions. Neotrypaea are also considered threats to the oyster industry because of their sediment-excavating activities. An expansion of their distribution beyond estuaries may have additional consequences for the Dungeness crab fishery (regionally valued at $33-74M/y) as Neotrypaea sp. are both competitors with juveniles and prey for larger Dungeness crab. This project will ground-truth predictions of Neotrypaea’s new offshore distribution with video and sample collections. Using box core samples we will document differences in potentially interacting benthic communities within the Neotrypaea beds as compared to areas not colonized by the shrimp. These new data are needed to determine whether the existing species composition is altered by the recruitment of Neotrypaea. We will estimate the shrimp’s contribution to benthic oxygen and nutrient fluxes by using aquatic eddy covariance (EC) methods and core incubations in shelf areas with and without abundant shrimp. This dataset includes the sampling locations for the model ground-truthing/community changes (box core collections) and the EC lander deployment and slow core collections. Coastal waters along the OR-WA shelf are subject to growing human related management, extractive, cultural, and recreational activities. This research is particularly needed for commercial fisheries stakeholders in decisions regarding ocean-use planning and be valuable to oyster growers concerned over burrowing shrimp pest management.
DOI: 10.26008/1912/bco-dmo.924852.1
title: Sample collection and sequence accession information for Zostera marina whole genome resequencing from specimens collected at 16 geographic locations worldwide in 2017

description: This dataset includes sample collection and sequence accession information for Zostera marina whole genome resequencing from specimens collected at 16 geographic locations worldwide in 2017. Sequence accessions are housed in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA).
DOI: 10.26008/1912/bco-dmo.924671.1
title: Temperature data recorded using HOBO Pendant MX2201 loggers deployed at 14 sites in Tomales Bay and Bodega Harbor during August 2019

description: To record water temperature, we deployed HOBO Pendant MX2201 loggers (fastened to PVC pipe) in the area from the 14 sites in Tomales and Bodega Harbors from which we collected genetic samples. The pipe was driven into the sediment until the logger was approximately &lt;15 centimeters (cm) above the sediment surface, positioned to rarely be emersed except during low spring tides. We recorded water temperature at 15-minute intervals during a two-week period at all sites from 16 August to 29 August in 2019.
DOI: 10.26008/1912/bco-dmo.924786.1
title: Sample collection information and sequence accessions at the National Center for Biotechnology Information (NCBI) for whole genome sequencing of eelgrass (Zostera marina) collected at Bodega and Tomales Bay, CA, USA from July to September 2019

description: This dataset includes sample collection information and sequence accessions at the National Center for Biotechnology Information (NCBI) for whole genome sequencing of eelgrass (Zostera marina) collected at Bodega and Tomales Bay, California, USA from July and September of 2019. Sequence Read Archive (SRA) Experiments and BioSamples can be accessed from the NCBI BioProject PRJNA887384 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA887384/).

Results summary as described in Scheibelhut, et al. (2023): We examine genomic signals of selection in the eelgrass Zostera marina across temperature gradients in adjacent embayments. Although we find many genomic regions with signals of selection within each bay there is very little overlap in signals of selection at the SNP level, despite most polymorphisms being shared across bays. We do find overlap at the gene level, potentially suggesting multiple mutational pathways to the same phenotype. Using polygenic models we find that some sets of candidate SNPs are able to predict temperature across both bays, suggesting that small but parallel shifts in allele frequencies may be missed by independent genome scans. Together, these results highlight the continuous rather than binary nature of parallel evolution in polygenic traits and the complexity of evolutionary predictability.
DOI: 10.26008/1912/bco-dmo.924808.1
title: Shoot measurements (sheath length and width) for the eelgrass (Zostera marina) shoots sampled for whole genome sequencing collected from Bodega and Tomales Bay, CA, USA from July to September 2019

description: This dataset provides shoot measurements for the shoots sampled for whole genome sequencing for Tomales Bay and Bodega Harbor (see related datasets for access to sequence data). Sheath width is measured on the terminal shoot using calipers at the abscission point (point where leaves emerge from the sheath). Sheath length is measured using a ruler to the nearest mm from the base to the abscission point. These data were used to record any differences in plant phenotype among the populations sampled.  Shoot Length was not measured since it is susceptible to breakage and density was not measured as we could not ascribe density to a genetic individual.
DOI: 10.26008/1912/bco-dmo.904417.1
title: Depth profiles of pore water constituents from sediment cores collected on the Louisiana Shelf of the Northern Gulf of Mexico during November 2020 on R/V Savannah cruise SAV-20-07

description: This data set reports depth profiles of pore water concentrations of dissolved inorganic carbon (DIC), orthophosphate (PO43-), ammonium (NH4+), total alkalinity (TA), carbon isotopic fractionation of DIC (13C-DIC), dissolved manganese (Mnd), dissolved calcium (Cad), reduced iron (Fe(II)), total dissolved Fe (Fed), and dissolved Fe(III) (Fe(III)d) by the difference of total dissolved Fe and Fe(II). These pore water data were obtained from sediment cores collected at five different stations on the Louisiana Shelf on the Northern Gulf of Mexico during the week of November 3-6, 2020 using an MC-800 multi-corer. These stations extend from the middle of the shelf offshore from Cocodrie, LA to the mouth of the Mississippi River North West Pass. All cores were processed within a few hours after collection. The field sampling was conducted during R/V Savannah cruise SAV-20-07.

The pore waters were first extracted by slicing sediment cores of &lt; 25 centimeters (cm) long and centrifugation of the pore waters under N2 atmosphere to avoid air contamination. Pore waters were then immediately filtered through 0.22-micrometer (um) PSE syringe filters under N2 atmosphere and either analyzed immediately onboard (Fe speciation, orthophosphate, DIC), preserved acidified with hydrochloric acid at 4 degrees Celsius (Cad, Mnd), frozen (ammonium), or preserved at 4 degrees Celsius after addition of HgCl2 (TA) until analysis. In addition, samples for TA and carbon isotopic analyses were preserved in glass bottles, whereas other samples were preserved in polypropylene containers. DIC was analyzed by flow injection analysis (Hall and Aller, 1992), orthophosphate, ammonium, and Fe speciation by spectrophotometry (Murphy and Riley, 1962; Strickland and Parsons, 1972; Stookey, 1970), TA by Gran titration (Gran, 1952),  the carbon isotopic signature by isotope ratio mass spectrometry (Wang et al, 2018), and Mnd and Cad by ICP-MS (Magette et al., 2023 In preparation). &quot;nd&quot; in the spreadsheet is provided when samples were not analyzed for these species (&quot;nd&quot; = not determined).
DOI: 10.26008/1912/bco-dmo.904782.1
title: Electrochemical data from sediment cores collected on the Louisiana Shelf of the Northern Gulf of Mexico during November 2020 on R/V Savannah cruise SAV-20-07

description: This data set reports sediment depth profiles of dissolved O2, Mn2+, Fe2+, and total dissolved sulfide (SH2S) concentrations, as well as pH and current intensities of organic-Fe(III) complexes and aqueous clusters of FeS (FeS(aq)) that cannot be quantified directly. These data were obtained by profiling intact sediment cores using voltammetric and pH microelectrodes deployed on a computer-operated micromanipulator. Sediment cores were collected at five different stations on the Louisiana Shelf on the Northern Gulf of Mexico during the week of November 3-6, 2020 using an MC-800 multi-corer. These stations extend from the middle of the shelf offshore from Cocodrie, LA to the mouth of the Mississippi River North West Pass. All depth profiles were started within 30 minutes after collection. The field sampling was conducted during R/V Savannah cruise SAV-20-07.
DOI: 10.26008/1912/bco-dmo.931248.1
title: Concentrations of dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater collected during the Indian GEOTRACES Transect (SK324; GI05) in September-October 2015

description: This dataset contains concentrations of dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater collected during the Indian GEOTRACES Transect (SK324; GI05; September 22 – October 21, 2015), in the western Indian Ocean, on the ORV Sagar Kanya. Similar data from SK312 (GI02) are available in a separate dataset. These data were generated at the Lamont-Doherty Earth Observatory of Columbia University (LDEO).
DOI: 10.26008/1912/bco-dmo.928222.1
title: In Situ Amphipod and Copepod Video Output Captured by the Hoodsport ORCA Profiling Mooring Mounted SPC-2 Zoocam in the Hood Canal, Puget Sound, Washington from August to September 2018 (Zooplankton Swimming project)

description: This dataset consists of videos of zooplankton swimming taken by an in-situ camera system (the SPC UW ZooCam) that was deployed on the Hoodsport ORCA profiling mooring in Hood Canal (Puget Sound), WA in summer 2018. Understanding zooplankton population dynamics is challenging, largely because traditional methods for quantifying zooplankton distributions are costly, limited in scope, and require extended analysis by trained analysts. We developed a novel methodology that combined remotely deployed camera systems, Machine Learning-based identification of zooplankton, and video-based tracking technology to quantify copepods’ and amphipods’ in situ swimming behaviors in a seasonally hypoxic and acidified fjord.
DOI: 10.26008/1912/bco-dmo.930347.1
title: Concentrations of dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater collected during the Indian GEOTRACES Transect (SK312; GI02) in April-May 2014

description: This dataset contains concentrations of dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater collected during the Indian GEOTRACES Transect (SK312; GI02; April 27 - May 30, 2014), in the western Indian Ocean, on the ORV Sagar Kanya. Similar data from SK324 (GI05) are available in a separate dataset. These data were generated at the Lamont-Doherty Earth Observatory of Columbia University (LDEO).