DOI: 10.5061/dryad.r4n35
title: Data from: Loss of growth homeostasis by genetic decoupling of cell division from biomass growth: implication for size control mechanisms

description: Growing cells adjust their division time with biomass accumulation to
 maintain growth homeostasis. Size control mechanisms, such as the size
 checkpoint, provide an inherent coupling of growth and division by gating
 certain cell cycle transitions based on cell size. We describe genetic
 manipulations that decouple cell division from cell size, leading to the
 loss of growth homeostasis, with cells becoming progressively smaller or
 progressively larger until arresting. This was achieved by modulating
 glucose influx independently of external glucose. Division rate followed
 glucose influx, while volume growth was largely defined by external
 glucose. Therefore, the coordination of size and division observed in
 wild‐type cells reflects tuning of two parallel processes, which is only
 refined by an inherent feedback‐dependent coupling. We present a class of
 size control models explaining the observed breakdowns of growth
 homeostasis.
description: wildtype cells grown in
 0%, 0.01%, 0.1% or 0.2% glucoseThis .nd2 file can be
 viewed with the Nikon Viewer, available at
 http://www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer. The file contains 80 fields of view (FOVs), images were taken every 10 minutes. The data corresponds to figures 1A, 1B and 1C. Wildtype cells were grown in SC media supplemented with either no glucose (FOV 1 to 20, up to timepoint 144), 0.01% glucose (FOV 21 to 40), 0.1% glucose (FOV 41 to 60) or 0.2% glucose (FOV 61 to 80).121205_WT.nd2wildtype cells grown 2% glucoseWildtype cells grown in SC with 2% glucose. Fields of view 27 to 41. Images were taken every 10 minutes. Corresponds to figure 1B, 1C.ma10_WT_KD_1condition001.nd2Transporterless cells with sensors in 0% glucoseTransporterless cells with sensors were grown in 0% glucose. Fields of view 1 to 20. Images were taken every 10 minutes.121017_sys_D1_Dox0.nd2Transporterless cells without sensors in 0% or 2% glucoseTransporterless cells without sensors were grown in 0% (Fields of view 1 to 20) or 2% (Fields of view 61 to 80) glucose. Corresponds to figures 1H (2%) and 1I (0%) Images were taken every 10 minutes.121123_Y1.nd2single-HXT2 with sensors in 0.1% glucose, 125ng/ml DOXsingle-HXT2 with sensors were grown in 0.1% glucose, with 125ng/ml DOX. Fields of view 41 to 60.121115_D2_Dox125ng002.nd2single-HXT2 with sensors in 0.1% glucose, 250ng/ml DOXsingle-HXT2 with sensors were grown in 0.1% glucose, with 250ng/ml DOX. Fields of view 41 to 60.121111_D2_Dox250ng.nd2single-HXT2 with sensors in 0.1% glucose, 1250ng/ml DOXsingle-HXT2 with sensors were grown in 0.1% glucose, with 1250ng/ml DOX. Field of view 41 to 60.121114_D2_Dox1p25ug001.nd2single-HXT2 with sensors in 0.1% glucose, 2500ng/ml DOXsingle-HXT2 with sensors were grown in 0.1% glucose, with 2500ng/ml DOX. Field of view 41 to 60.121113_D2_Dox2p5ug.nd2single-HXT2 without sensors in 0.1% glucose, no DOXsingle-HXT2 without sensors were grown in 0.1% glucose, with DOX. Fields of view 1 to 20130116_Y4noDox.nd2single-HXT2 without sensors in 0.1% glucose, 2500 ng/ml DOX AND single-HXT4 without sensors in 0.1% or 2% glucose 2500ng/ml DOXSingle-HXT2 without sensors were grown in 0.1% glucose, with 2500ng/ml DOX. Field of view 41 to 60. Single-HXT4 without sensors were grown in 0.1% (Fields of view 41 to 60) or 2% (Fields of view 61 to 80) glucose, with 2500ng/ml DOX.130121_Y4_2p5Dox001.nd2