DOI: 10.5061/dryad.gmsbcc2v0
title: Experimental peritoneal dialysis in Sprague-Dawley rats using conventional or low sodium dialysis fluids

description: During peritoneal dialysis, sodium is transferred in a paracellular
 fashion via inter-endothelial nanochannels from the blood plasma water to
 the dialysate and, initially, dialysate sodium concentrations are also
 diluted by free water transport across endothelial aquaporin-1 channels.
 These experiments were performed to evaluate the relative contributions of
 free water transport, convective- and diffusive transfer on dialysate
 sodium. Based on our findings, we devised a simple method to quantify
 ultrafiltration by analyzing the initial decline in dialysate sodium
 concentration, commonly referred to as the "sodium dip."
description: Experimental peritoneal dialysis was performed in healthy
 anesthetized 9 weeks old Sprague-Dawley rats using either 20 mL
 conventional (134 mmol/L) or low-sodium (125 mmol/L) peritoneal dialysis
 fluids utilizing a dwell time of 120 minutes. Blood plasma was analysed
 before and after dialysis to measure Na<sup>+</sup>,
 K<sup>+,</sup> Cl<sup>-</sup>.
 Ca<sup>++</sup>, glucose, urea, creatinine, and total
 CO<sub>2</sub>. <sup>125</sup>I-albumin and
 <sup>51</sup>Cr-EDTA were added to the dialysate to estimate
 intra-peritoneal volume and to assess small solute diffusion capacity,
 respectively. Samples (20 μL) of dialysate and blood were collected at
 multiple time-points during the dialysis session and analyzed on a gamma
 counter. All data in this dataset are unprocessed.
description: # Experimental peritoneal dialysis in Sprague-Dawley rats using
 conventional or low sodium dialysis fluids ## GENERAL INFORMATION 1. Title
 of Dataset: Experimental Peritoneal Dialysis in Sprague Dawley Rats: Low
 Sodium versus Conventional Dialysis Fluids 2. Author Information A.
 Principal Investigator Contact Information Name: Carl berg Institution:
 Lund University, Medical Faculty Address: Box 117, SE-221 00 Email:
 3. Date of data collection March 2022 to
 April 2022 4. Geographic location of data collection: Lund, Sweden 5.
 Information about funding sources that supported the collection of the
 data: Funding by Lund University Medical Faculty Foundation grant YF
 2020-YF0056, and The Inga-Britt and Arne Lundbergs Research Foundation
 (all to C.M. berg). ## SHARING/ACCESS INFORMATION 1. Licenses/restrictions
 placed on the data: - 2. Links to publications that cite or use the data:
 - 3. Links to other publicly accessible locations of the data: - 4.
 Links/relationships to ancillary data sets: - 5. Was data derived from
 another source? No 6. Recommended citation for this dataset: - ## DATA
 & FILE OVERVIEW 1. File List: lowna.csv 2. Relationship between files,
 if important: - 3. Additional related data collected that was not included
 in the current data package: - 4. Are there multiple versions of the
 dataset? NO ## METHODOLOGICAL INFORMATION 1. Description of methods used
 for collection/generation of data: - 2. Methods for processing the data: -
 3. Instrument- or software-specific information needed to interpret the
 data: - 4. Standards and calibration information, if appropriate: - 5.
 Environmental/experimental conditions: - 6. Describe any quality-assurance
 procedures performed on the data: - 7. People involved with sample
 collection, processing, analysis and/or submission: - ## DATA-SPECIFIC
 INFORMATION FOR: lowna.csv 1. Number of variables: 148 2. Number of
 cases/rows: 24 3. Variable List: animal, "unique number assigned to
 each animal" treatment, "Dialysis fluid used, e.g. LowNa 1.5%
 represents low sodium and 1.5% glucose strength" weight, "Body
 weight in grams before dialysis" date, "Date of the experiment
 YYYY-MM-DD" vin, "Peritoneal dialysis fluid volume instilled in
 milliliters" vout, "Peritoneal dialysis fluid volume extracted
 in milliliters (see manuscript for details)" wo_in, "Wash-out
 fluid instilled (same type of fluid as used for dialysis) in
 milliliters" wo_out, "Wash-out fluid extracted in
 milliliters" wo_alb, "Wash-out fluid 125-I activity in
 Bq/mL" p_na_before, "Plasma sodium concentration before dialysis
 in mmol/L" p_k_before, "Plasma potassium concentration before
 dialysis in mmol/L" p_cl_before, "Plasma chloride concentration
 before dialysis in mmol/L" p_ica_before, "Plasma ionized calcium
 concentration before dialysis in mmol/L" p_tco2_before, "Plasma
 total carbon dioxide concentration before dialysis in mmol/L"
 p_glu_before, "Plasma glucose concentration before dialysis in
 mmol/L" p_urea_before, "Plasma urea concentration before
 dialysis in mmol/L" p_crea_before, "Plasma creatinine
 concentration before dialysis in mmol/L" map_before, "Mean
 arterial pressure before dialysis in mmol/L" hr_before, "Heart
 rate before dialysis in mmol/L" p_na_after, "Plasma sodium
 concentration after dialysis in mmol/L" p_k_after, "Plasma
 potassium concentration after dialysis in mmol/L" p_cl_after,
 "Plasma chloride concentration after dialysis in mmol/L"
 p_ica_after, "Plasma ionized calcium concentration after dialysis in
 mmol/L" p_tco2_after, "Plasma total carbon dioxide concentration
 before dialysis in mmol/L" p_glu_after, "Plasma glucose
 concentration after dialysis in mmol/L" p_urea_after, "Plasma
 urea concentration after dialysis in mmol/L" p_crea_after,
 "Plasma creatinine concentration after dialysis in mmol/L"
 map_after, "Mean arterial pressure before dialysis in mmol/L"
 hr_after, "Heart rate before dialysis in mmol/L" n_p_samples,
 "Number of blood samples obtained during dialysis" pt1,
 "Time point for blood sample #1 in minutes post start of
 dialysis" pt2, "Time point for blood sample #2 in minutes post
 start of dialysis" pt3, "Time point for blood sample #3 in
 minutes post start of dialysis" pt4, "Time point for blood
 sample #4 in minutes post start of dialysis" pt5, "Time point
 for blood sample #5 in minutes post start of dialysis" pt6,
 "Time point for blood sample #6 in minutes post start of
 dialysis" p_hct_1, "Hematocrit for blood sample #1"
 p_hct_2, "Hematocrit for blood sample #2" p_hct_3,
 "Hematocrit for blood sample #3" p_hct_4, "Hematocrit for
 blood sample #4" p_hct_5, "Hematocrit for blood sample #5"
 p_hct_6, "Hematocrit for blood sample #6" p_edta_1, "Plasma
 51Cr activity (blood sample #1) in Bq/mL" p_edta_2, "Plasma 51Cr
 activity (blood sample #2) in Bq/mL" p_edta_3, "Plasma 51Cr
 activity (blood sample #3) in Bq/mL" p_edta_4, "Plasma 51Cr
 activity (blood sample #4) in Bq/mL" p_edta_5, "Plasma 51Cr
 activity (blood sample #5) in Bq/mL" p_edta_6, "Plasma 51Cr
 activity (blood sample #6) in Bq/mL" p_alb_1, "Plasma 125I
 activity (blood sample #1) in Bq/mL" p_alb_2, "Plasma 125I
 activity (blood sample #2) in Bq/mL" p_alb_3, "Plasma 125I
 activity (blood sample #3) in Bq/mL" p_alb_4, "Plasma 125I
 activity (blood sample #4) in Bq/mL" p_alb_5, "Plasma 125I
 activity (blood sample #5) in Bq/mL" p_alb_6, "Plasma 125I
 activity (blood sample #6) in Bq/mL" n_d_samples, "Number of
 dialysate samples obtained during dialysis" d_sv_1, "Sampling
 volume for dialysate sample #1 in milliliters" d_sv_2, "Sampling
 volume for dialysate sample #2 in milliliters" d_sv_3, "Sampling
 volume for dialysate sample #3 in milliliters" d_sv_4, "Sampling
 volume for dialysate sample #4 in milliliters" d_sv_5, "Sampling
 volume for dialysate sample #5 in milliliters" d_sv_6, "Sampling
 volume for dialysate sample #6 in milliliters" d_sv_7, "Sampling
 volume for dialysate sample #7 in milliliters" d_edta_0, "51Cr
 activity in fresh dialysis fluid (before instillation) in Bq/mL"
 d_edta_1, "51Cr activity in dialysate sample #1 in Bq/mL"
 d_edta_2, "51Cr activity in dialysate sample #2 in Bq/mL"
 d_edta_3, "51Cr activity in dialysate sample #3 in Bq/mL"
 d_edta_4, "51Cr activity in dialysate sample #4 in Bq/mL"
 d_edta_5, "51Cr activity in dialysate sample #5 in Bq/mL"
 d_edta_6, "51Cr activity in dialysate sample #6 in Bq/mL"
 d_edta_7, "51Cr activity in dialysate sample #7 in Bq/mL"
 d_alb_0, "125I activity in fresh dialysis fluid (before instillation)
 in Bq/mL" d_alb_1, "125I activity in dialysate sample #1 in
 Bq/mL" d_alb_2, "125I activity in dialysate sample #2 in
 Bq/mL" d_alb_3, "125I activity in dialysate sample #3 in
 Bq/mL" d_alb_4, "125I activity in dialysate sample #4 in
 Bq/mL" d_alb_5, "125I activity in dialysate sample #5 in
 Bq/mL" d_alb_6, "125I activity in dialysate sample #6 in
 Bq/mL" d_alb_7, "125I activity in dialysate sample #7 in
 Bq/mL" d_glu_0, "Measured glucose concentration in fresh
 dialysis fluid (before instillation) in mmol/L" d_glu_1,
 "Measured glucose concentration in dialysate sample #1 in
 mmol/L" d_glu_2, "Measured glucose concentration in dialysate
 sample #2 in mmol/L" d_glu_3, "Measured glucose concentration in
 dialysate sample #3 in mmol/L" d_glu_4, "Measured glucose
 concentration in dialysate sample #4 in mmol/L" d_glu_5,
 "Measured glucose concentration in dialysate sample #5 in
 mmol/L" d_glu_6, "Measured glucose concentration in dialysate
 sample #6 in mmol/L" d_glu_7, "Measured glucose concentration in
 dialysate sample #7 in mmol/L" d_na_0, "Measured sodium
 concentration in fresh dialysis fluid (before instillation) in
 mmol/L" d_na_1, "Measured glucose concentration in dialysate
 sample #1 in mmol/L" d_na_2, "Measured glucose concentration in
 dialysate sample #2 in mmol/L" d_na_3, "Measured glucose
 concentration in dialysate sample #3 in mmol/L" d_na_4,
 "Measured glucose concentration in dialysate sample #4 in
 mmol/L" d_na_5, "Measured glucose concentration in dialysate
 sample #5 in mmol/L" d_na_6, "Measured glucose concentration in
 dialysate sample #6 in mmol/L" d_na_7, "Measured glucose
 concentration in dialysate sample #7 in mmol/L" d_k_0, "Measured
 potassium concentration in fresh dialysis fluid (before instillation) in
 mmol/L" d_k_1, "Measured potassium concentration in dialysate
 sample #1 in mmol/L" d_k_2, "Measured potassium concentration in
 dialysate sample #2 in mmol/L" d_k_3, "Measured potassium
 concentration in dialysate sample #3 in mmol/L" d_k_4, "Measured
 potassium concentration in dialysate sample #4 in mmol/L" d_k_5,
 "Measured potassium concentration in dialysate sample #5 in
 mmol/L" d_k_6, "Measured potassium concentration in dialysate
 sample #6 in mmol/L" d_k_7, "Measured potassium concentration in
 dialysate sample #7 in mmol/L" d_cl_0, "Measured chloride
 concentration in fresh dialysis fluid (before instillation) in
 mmol/L" d_cl_1, "Measured chloride concentration in dialysate
 sample #1 in mmol/L" d_cl_2, "Measured chloride concentration in
 dialysate sample #2 in mmol/L" d_cl_3, "Measured chloride
 concentration in dialysate sample #3 in mmol/L" d_cl_4,
 "Measured chloride concentration in dialysate sample #4 in
 mmol/L" d_cl_5, "Measured chloride concentration in dialysate
 sample #5 in mmol/L" d_cl_6, "Measured chloride concentration in
 dialysate sample #6 in mmol/L" d_cl_7, "Measured chloride
 concentration in dialysate sample #7 in mmol/L" d_ica_0,
 "Measured ionized calcium concentration in fresh dialysis fluid
 (before instillation) in mmol/L" d_ica_1, "Measured ionized
 calcium concentration in dialysate sample #1 in mmol/L" d_ica_2,
 "Measured ionized calcium concentration in dialysate sample #2 in
 mmol/L" d_ica_3, "Measured ionized calcium concentration in
 dialysate sample #3 in mmol/L" d_ica_4, "Measured ionized
 calcium concentration in dialysate sample #4 in mmol/L" d_ica_5,
 "Measured ionized calcium concentration in dialysate sample #5 in
 mmol/L" d_ica_6, "Measured ionized calcium concentration in
 dialysate sample #6 in mmol/L" d_ica_7, "Measured ionized
 calcium concentration in dialysate sample #7 in mmol/L" d_tco2_0,
 "Measured total carbon dioxide concentration in fresh dialysis fluid
 (before instillation) in mmol/L" d_tco2_1, "Measured total
 carbon dioxide concentration in dialysate sample #1 in mmol/L"
 d_tco2_2, "Measured total carbon dioxide concentration in dialysate
 sample #1 in mmol/L" d_tco2_3, "Measured total carbon dioxide
 concentration in dialysate sample #1 in mmol/L" d_tco2_4,
 "Measured total carbon dioxide concentration in dialysate sample #1
 in mmol/L" d_tco2_5, "Measured total carbon dioxide
 concentration in dialysate sample #1 in mmol/L" d_tco2_6,
 "Measured total carbon dioxide concentration in dialysate sample #1
 in mmol/L" d_tco2_7, "Measured total carbon dioxide
 concentration in dialysate sample #1 in mmol/L" d_urea_1,
 "Measured urea concentration in dialysate sample #1 in mmol/L"
 d_urea_2, "Measured urea concentration in dialysate sample #2 in
 mmol/L" d_urea_3, "Measured urea concentration in dialysate
 sample #3 in mmol/L" d_urea_4, "Measured urea concentration in
 dialysate sample #4 in mmol/L" d_urea_5, "Measured urea
 concentration in dialysate sample #5 in mmol/L" d_urea_6,
 "Measured urea concentration in dialysate sample #6 in mmol/L"
 d_urea_7, "Measured urea concentration in dialysate sample #7 in
 mmol/L" d_crea_0, "Measured creatinine concentration in fresh
 dialysis fluid (before instillation) in mol/L" d_crea_1,
 "Measured creatinine concentration in dialysate sample #2 in
 mol/L" d_crea_2, "Measured creatinine concentration in dialysate
 sample #2 in mol/L" d_crea_3, "Measured creatinine concentration
 in dialysate sample #2 in mol/L" d_crea_4, "Measured creatinine
 concentration in dialysate sample #2 in mol/L" d_crea_5,
 "Measured creatinine concentration in dialysate sample #2 in
 mol/L" d_crea_6, "Measured creatinine concentration in dialysate
 sample #2 in mol/L" d_crea_7, "Measured creatinine concentration
 in dialysate sample #2 in mol/L" dt1, "Time point for dialysate
 sample #1 in minutes post start of dialysis" dt2, "Time point
 for dialysate sample #2 in minutes post start of dialysis" dt3,
 "Time point for dialysate sample #3 in minutes post start of
 dialysis" dt4, "Time point for dialysate sample #4 in minutes
 post start of dialysis" dt5, "Time point for dialysate sample #5
 in minutes post start of dialysis" dt6, "Time point for
 dialysate sample #6 in minutes post start of dialysis" dt7,
 "Time point for dialysate sample #7 in minutes post start of
 dialysis" 1. Missing data codes: NA 2. Specialized formats or other
 abbreviations used: -