cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7835357
title: Sowing operation direction and population densities in corn growing

description: ABSTRACT Among the factors that may influence corn yield are sowing operation direction and plant population, since they alter the shade index between crop rows, and consequently the efficiency of interception of the photosynthetically active radiation by the leaves of plants. The aim of this study was to evaluate the influence of sowing direction and plant populations on P2830VYH hybrid corn yield. The experiment was conducted at the facilities of FCAV-UNESP, latitude 21º 14’ S and longitude 48º 16’ W in Jaboticabal (SP). The treatments were composed of three sowing directions (North-South, Northeast-Southwest, and East-West) and three population densities (40,000, 60,000 and 80,000 plants ha-1). The treatment with 80,000 plants ha-1 had the lowest cost per sack of corn and profitability 21% higher than that of the treatment with 60,000 plants ha-1, and 45% higher than that of the treatment with 40,000 plants ha-1. Sowing direction had no influence on grain yield when environmental conditions were favorable to the development of the crop. Lower plant densities lead to higher individual results in relation to the number of cobs, length of cobs and number of grains per row.
DOI: 10.6084/m9.figshare.7835351.v1
title: Family farming and school meals in Rio Grande do Sul, Brazil

description: ABSTRACT: In Brazil, at least 30% of the total financial resources provided by the federal government for school feeding should be used to buy products from family farming. The present study examines the profile of the purchase of these products in municipalities of the state of Rio Grande do Sul (RS), south Brazil, in 2014. A cross-sectional descriptive study was carried out with information obtained through a questionnaire sent to the municipalities and filled by respondents. Of the 371 participating municipalities, 367 acquired products from family farming, and 86.8% of these municipalities achieved the minimum percentage required. Nutritionists and farmers were the main components involved in the articulation of this process. Difficulties faced by these individuals included the lack of organization of the producers, failure to meet the demand, and lack of interest. More than 80% of the municipalities bought food from the “Vegetables”, “Cereals, Breads, Pasta, and Tubers” and “Fruits” groups. Only 8% purchased products from socio-biodiversity. There was a great diversity of food purchased, most of which were in natura, despite the low acquisition of socio-biodiversity products. Results of this study indicated the concern and tendency of the Brazilian municipalities to support the local economy with incentives to consumption and production of regional foods.
DOI: 10.6084/m9.figshare.7835357.v1
title: Sowing operation direction and population densities in corn growing

description: ABSTRACT Among the factors that may influence corn yield are sowing operation direction and plant population, since they alter the shade index between crop rows, and consequently the efficiency of interception of the photosynthetically active radiation by the leaves of plants. The aim of this study was to evaluate the influence of sowing direction and plant populations on P2830VYH hybrid corn yield. The experiment was conducted at the facilities of FCAV-UNESP, latitude 21º 14’ S and longitude 48º 16’ W in Jaboticabal (SP). The treatments were composed of three sowing directions (North-South, Northeast-Southwest, and East-West) and three population densities (40,000, 60,000 and 80,000 plants ha-1). The treatment with 80,000 plants ha-1 had the lowest cost per sack of corn and profitability 21% higher than that of the treatment with 60,000 plants ha-1, and 45% higher than that of the treatment with 40,000 plants ha-1. Sowing direction had no influence on grain yield when environmental conditions were favorable to the development of the crop. Lower plant densities lead to higher individual results in relation to the number of cobs, length of cobs and number of grains per row.
DOI: 10.6084/m9.figshare.7835345
title: Influence of Pregnancy on the Inflammatory Process Following Direct Pulp Capping: a Preliminary Study in Rats

description: Abstract The purpose of this study was to evaluate the inflammatory process following direct pulp capping during pregnancy. This experimental study involved 48 maxillary first molars of female Wistar rats. The procedures were performed in pregnant and non-pregnant animals (n =20 each). Direct pulp capping with mineral trioxide aggregate (MTA) and restoration with a light-cured resin composite was performed in half of exposed pulp specimens. In the other half of specimens, light-cured composite was placed directly on the exposed pulp. In the control groups (n=4 each), no intervention was performed. Animals were euthanized at 3 and 7 days. All sections (three per slide) were viewed under an optical microscope. One previously calibrated pathologist performed descriptive analysis and assigned scores for inflammatory response and tissue organization adjacent to the pulp exposure. The Kappa value for intra-examiner variability was 0.91. At 3 days, in animals treated with MTA, inflammatory infiltrate was absent in non-pregnant animals while mild inflammatory infiltrate was observed in some pregnant animals. The inflammatory response ranged from mild to severe in both groups treated with composite alone. At 7 days, the inflammatory response was more intense in pregnant than in non-pregnant animals treated with MTA; while this difference were not evident in animals treated with composite alone. In conclusion, pregnancy may not influence the inflammatory process following direct pulp capping with light-cured resin composite, which was always harmful to the pulp; while the tissue response after the direct pulp with MTA were more favorable in non-pregnant animals.
DOI: 10.6084/m9.figshare.7835348
title: Pharmacokinetics of neutron-irradiated meglumine antimoniate in Leishmania amazonensis-infected BALB/c mice

description: Abstract Background: Cutaneous leishmaniasis (CL) is a parasitic disease caused by the protozoan Leishmania spp. Pentavalent antimonial agents have been used as an effective therapy, despite their side effects and resistant cases. Their pharmacokinetics remain largely unexplored. This study aimed to investigate the pharmacokinetic profile of meglumine antimoniate in a murine model of cutaneous leishmaniasis using a radiotracer approach. Methods: Meglumine antimoniate was neutron-irradiated inside a nuclear reactor and was administered once intraperitoneally to uninfected and L. amazonensis-infected BALB/c mice. Different organs and tissues were collected and the total antimony was measured. Results: Higher antimony levels were found in infected than uninfected footpad (0.29% IA vs. 0.14% IA, p = 0.0057) and maintained the concentration. The animals accumulated and retained antimony in the liver, which cleared slowly. The kidney and intestinal uptake data support the hypothesis that antimony has two elimination pathways, first through renal excretion, followed by biliary excretion. Both processes demonstrated a biphasic elimination profile classified as fast and slow. In the blood, antimony followed a biexponential open model. Infected mice showed a lower maximum concentration (6.2% IA/mL vs. 11.8% IA/mL, p = 0.0001), a 2.5-fold smaller area under the curve, a 2.7-fold reduction in the mean residence time, and a 2.5-fold higher clearance rate when compared to the uninfected mice. Conclusions: neutron-irradiated meglumine antimoniate concentrates in infected footpad, while the infection affects antimony pharmacokinetics.
DOI: 10.6084/m9.figshare.7835339
title: Abiotic effects on the cover and richness of corticolous lichens on Araucaria angustifolia trunks

description: ABSTRACT Lichens play a key role in natural ecosystems, as they can function as primary producers, recycle minerals and fix nitrogen. Despite their environmental importance, little is known about lichen ecology in Brazil, and especially about how abiotic factors may influence their spatial distribution. In this study, we aimed to verify how the cover and richness of corticolous lichens on Araucaria angustifolia trunks vary between two different habitats (Forest and Grassland). The photoquadrat sampling method was applied to A. angustifolia trunks. The Coral Point Count software with Excel extensions (CPCe) was used to analyze photographs for lichen cover and richness. Additionally, a redundancy analysis was conducted to estimate how five abiotic and two biotic variables affected the spatial distribution of lichens. Twenty-five morphospecies were identified, none of them occurring exclusively in the Grassland habitat. Canopy openness, air humidity and tree trunk rugosity were important parameters influencing lichen distribution; therefore, spatial segregation of growth forms can be explained by environmental selectivity. Foliose lichens require more air humidity, which explains their predominance in the Forest habitat. Canopy openness in Grassland habitat favors fruticose lichens, which depend on factors such as wind for reproduction.
DOI: 10.6084/m9.figshare.7835348.v1
title: Pharmacokinetics of neutron-irradiated meglumine antimoniate in Leishmania amazonensis-infected BALB/c mice

description: Abstract Background: Cutaneous leishmaniasis (CL) is a parasitic disease caused by the protozoan Leishmania spp. Pentavalent antimonial agents have been used as an effective therapy, despite their side effects and resistant cases. Their pharmacokinetics remain largely unexplored. This study aimed to investigate the pharmacokinetic profile of meglumine antimoniate in a murine model of cutaneous leishmaniasis using a radiotracer approach. Methods: Meglumine antimoniate was neutron-irradiated inside a nuclear reactor and was administered once intraperitoneally to uninfected and L. amazonensis-infected BALB/c mice. Different organs and tissues were collected and the total antimony was measured. Results: Higher antimony levels were found in infected than uninfected footpad (0.29% IA vs. 0.14% IA, p = 0.0057) and maintained the concentration. The animals accumulated and retained antimony in the liver, which cleared slowly. The kidney and intestinal uptake data support the hypothesis that antimony has two elimination pathways, first through renal excretion, followed by biliary excretion. Both processes demonstrated a biphasic elimination profile classified as fast and slow. In the blood, antimony followed a biexponential open model. Infected mice showed a lower maximum concentration (6.2% IA/mL vs. 11.8% IA/mL, p = 0.0001), a 2.5-fold smaller area under the curve, a 2.7-fold reduction in the mean residence time, and a 2.5-fold higher clearance rate when compared to the uninfected mice. Conclusions: neutron-irradiated meglumine antimoniate concentrates in infected footpad, while the infection affects antimony pharmacokinetics.
DOI: 10.6084/m9.figshare.7835333
title: Comparison of equipment for grain sampling

description: ABSTRACT Grain sampling requires the use of appropriate and accurate equipment. This study aimed to compare grain samplers, manual and mechanical, used in the sampling of soybean loads, during their reception by storage units. The used devices were the manual sampler with 1.80 m length and three opening stages, 2.10 m length and three opening stages; and 2.10 m length and one opening stage, besides the mechanical sampler (pneumatic) and the pelican sampler. The analyzed parameters were the contents of impurity, broken grains, pods, immature grains, and moisture. The significance of effect of treatment was determined by F Test and the means were compared by Tukey test (p &lt; 0.05). The devices used for sampling of soybean grains in vehicles, during their reception by storage units, affect the determination of broken grains, pods and immature grains. However, there was no difference between the types of sampling equipment in the determination of impurity content, and the pelican sampler collected greater percentages of pods and immature grains from the sampled vehicles.
DOI: 10.6084/m9.figshare.7835336
title: Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

description: ABSTRACT Presence of toxic compounds in marine coastal waters has increased exponentially since Industrial Revolution. In this way, we aimed to evaluate biochemical and physiological changes occurring within Hypnea musciformis after short-term exposure to gasoline. Hypnea musciformis was cultivated without gasoline and then exposed to various concentrations of it (0.001 % - 1.0 %, v/v) for periods of 30 min, 1 h, 12 h and 24 h. A Pricncipal Compound Analysis of UV-vis spectral window (200-700 nm) was able to discriminate gasoline-exposed samples according to both exposure time and gasoline concentration. Changes in carotenoid profile composition were observed. Decreased carotenoid content was associated to gasoline exposure time, being lutein and trans-β-carotene the major compounds found. Higher gasoline concentrations negatively interfered with phenolic compounds accumulation. In addition, increased gasoline concentrations corresponded to decreased intracellular starch grains content as well as increased its deposition on cell wall external surface. Data obtained allow us to conclude that gasoline can damage Hypnea musciformis physiology and cell morphology. This is important, considering Hypnea musciformis carotenoids and phenolics are potential biomarkers of environmental stress investigated, as well as its increased cell wall thickness to avoid gasoline diffusion.
DOI: 10.6084/m9.figshare.7835345.v1
title: Influence of Pregnancy on the Inflammatory Process Following Direct Pulp Capping: a Preliminary Study in Rats

description: Abstract The purpose of this study was to evaluate the inflammatory process following direct pulp capping during pregnancy. This experimental study involved 48 maxillary first molars of female Wistar rats. The procedures were performed in pregnant and non-pregnant animals (n =20 each). Direct pulp capping with mineral trioxide aggregate (MTA) and restoration with a light-cured resin composite was performed in half of exposed pulp specimens. In the other half of specimens, light-cured composite was placed directly on the exposed pulp. In the control groups (n=4 each), no intervention was performed. Animals were euthanized at 3 and 7 days. All sections (three per slide) were viewed under an optical microscope. One previously calibrated pathologist performed descriptive analysis and assigned scores for inflammatory response and tissue organization adjacent to the pulp exposure. The Kappa value for intra-examiner variability was 0.91. At 3 days, in animals treated with MTA, inflammatory infiltrate was absent in non-pregnant animals while mild inflammatory infiltrate was observed in some pregnant animals. The inflammatory response ranged from mild to severe in both groups treated with composite alone. At 7 days, the inflammatory response was more intense in pregnant than in non-pregnant animals treated with MTA; while this difference were not evident in animals treated with composite alone. In conclusion, pregnancy may not influence the inflammatory process following direct pulp capping with light-cured resin composite, which was always harmful to the pulp; while the tissue response after the direct pulp with MTA were more favorable in non-pregnant animals.
DOI: 10.6084/m9.figshare.7835330
title: BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells

description: Abstract Background: The use of animal venoms and their toxins as material sources for biotechnological applications has received much attention from the pharmaceutical industry. L-amino acid oxidases from snake venoms (SV-LAAOs) have demonstrated innumerous biological effects and pharmacological potential against different cancer types. Hepatocellular carcinoma has increased worldwide, and the aberrant DNA methylation of liver cells is a common mechanism to promote hepatic tumorigenesis. Moreover, tumor microenvironment plays a major role in neoplastic transformation. To elucidate the molecular mechanisms responsible for the cytotoxic effects of SV-LAAO in human cancer cells, this study aimed to evaluate the cytotoxicity and the alterations in DNA methylation profiler in the promoter regions of cell-cycle genes induced by BjussuLAAO-II, an LAAO from Bothrops jaracussu venom, in human hepatocellular carcinoma (HepG2) cells in monoculture and co-culture with endothelial (HUVEC) cells. Methods: BjussuLAAO-II concentrations were 0.25, 0.50, 1.00 and 5.00 μg/mL. Cell viability was assessed by MTT assay and DNA methylation of the promoter regions of 22 cell-cycle genes by EpiTect Methyl II PCR array. Results: BjussuLAAO-II decreased the cell viability of HepG2 cells in monoculture at all concentrations tested. In co-culture, 1.00 and 5.00 μg/mL induced cytotoxicity (p &lt; 0.05). BjussuLAAO-II increased the methylation of CCND1 and decreased the methylation of CDKN1A in monoculture and GADD45A in both cell-culture models (p &lt; 0.05). Conclusion: Data showed BjussuLAAO-II induced cytotoxicity and altered DNA methylation of the promoter regions of cell-cycle genes in HepG2 cells in monoculture and co-culture models. We suggested the analysis of DNA methylation profile of GADD45A as a potential biomarker of the cell cycle effects of BjussuLAAO-II in cancer cells. The tumor microenvironment should be considered to comprise part of biotechnological strategies during the development of snake-toxin-based novel drugs.
DOI: 10.6084/m9.figshare.7835336.v1
title: Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

description: ABSTRACT Presence of toxic compounds in marine coastal waters has increased exponentially since Industrial Revolution. In this way, we aimed to evaluate biochemical and physiological changes occurring within Hypnea musciformis after short-term exposure to gasoline. Hypnea musciformis was cultivated without gasoline and then exposed to various concentrations of it (0.001 % - 1.0 %, v/v) for periods of 30 min, 1 h, 12 h and 24 h. A Pricncipal Compound Analysis of UV-vis spectral window (200-700 nm) was able to discriminate gasoline-exposed samples according to both exposure time and gasoline concentration. Changes in carotenoid profile composition were observed. Decreased carotenoid content was associated to gasoline exposure time, being lutein and trans-β-carotene the major compounds found. Higher gasoline concentrations negatively interfered with phenolic compounds accumulation. In addition, increased gasoline concentrations corresponded to decreased intracellular starch grains content as well as increased its deposition on cell wall external surface. Data obtained allow us to conclude that gasoline can damage Hypnea musciformis physiology and cell morphology. This is important, considering Hypnea musciformis carotenoids and phenolics are potential biomarkers of environmental stress investigated, as well as its increased cell wall thickness to avoid gasoline diffusion.
DOI: 10.6084/m9.figshare.7835339.v1
title: Abiotic effects on the cover and richness of corticolous lichens on Araucaria angustifolia trunks

description: ABSTRACT Lichens play a key role in natural ecosystems, as they can function as primary producers, recycle minerals and fix nitrogen. Despite their environmental importance, little is known about lichen ecology in Brazil, and especially about how abiotic factors may influence their spatial distribution. In this study, we aimed to verify how the cover and richness of corticolous lichens on Araucaria angustifolia trunks vary between two different habitats (Forest and Grassland). The photoquadrat sampling method was applied to A. angustifolia trunks. The Coral Point Count software with Excel extensions (CPCe) was used to analyze photographs for lichen cover and richness. Additionally, a redundancy analysis was conducted to estimate how five abiotic and two biotic variables affected the spatial distribution of lichens. Twenty-five morphospecies were identified, none of them occurring exclusively in the Grassland habitat. Canopy openness, air humidity and tree trunk rugosity were important parameters influencing lichen distribution; therefore, spatial segregation of growth forms can be explained by environmental selectivity. Foliose lichens require more air humidity, which explains their predominance in the Forest habitat. Canopy openness in Grassland habitat favors fruticose lichens, which depend on factors such as wind for reproduction.
DOI: 10.6084/m9.figshare.7835333.v1
title: Comparison of equipment for grain sampling

description: ABSTRACT Grain sampling requires the use of appropriate and accurate equipment. This study aimed to compare grain samplers, manual and mechanical, used in the sampling of soybean loads, during their reception by storage units. The used devices were the manual sampler with 1.80 m length and three opening stages, 2.10 m length and three opening stages; and 2.10 m length and one opening stage, besides the mechanical sampler (pneumatic) and the pelican sampler. The analyzed parameters were the contents of impurity, broken grains, pods, immature grains, and moisture. The significance of effect of treatment was determined by F Test and the means were compared by Tukey test (p &lt; 0.05). The devices used for sampling of soybean grains in vehicles, during their reception by storage units, affect the determination of broken grains, pods and immature grains. However, there was no difference between the types of sampling equipment in the determination of impurity content, and the pelican sampler collected greater percentages of pods and immature grains from the sampled vehicles.
DOI: 10.6084/m9.figshare.7835318
title: Fire records in tree rings of Moquiniastrum polymorphum: potential for reconstructing fire history in the Brazilian Atlantic Forest

description: ABSTRACT Fire disturbance affects the composition, structure and dynamics of vegetation. Historical records of fire events exist in some places, but they are generally limited in temporal and spatial extent. Tree-ring research is a useful tool for fire history reconstruction and can contribute important long-term ecological data. We tested the hypotheses that Moquiniastrum polymorphum (Less.) G. Sancho, a widespread species in Brazil that occurs in burnt areas of Atlantic Forest, produces annual growth rings and that its wood can record fire incidence by datable fire scaring. Our results corroborate these hypotheses and indicate that the species has potential for fire history reconstruction.
DOI: 10.6084/m9.figshare.7835318.v1
title: Fire records in tree rings of Moquiniastrum polymorphum: potential for reconstructing fire history in the Brazilian Atlantic Forest

description: ABSTRACT Fire disturbance affects the composition, structure and dynamics of vegetation. Historical records of fire events exist in some places, but they are generally limited in temporal and spatial extent. Tree-ring research is a useful tool for fire history reconstruction and can contribute important long-term ecological data. We tested the hypotheses that Moquiniastrum polymorphum (Less.) G. Sancho, a widespread species in Brazil that occurs in burnt areas of Atlantic Forest, produces annual growth rings and that its wood can record fire incidence by datable fire scaring. Our results corroborate these hypotheses and indicate that the species has potential for fire history reconstruction.