cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2011.217
title: Preparation of polyethylenemine-coated carbon nanotubed modified with methyl thioglycolate for extraction of gold (III) ions

description: Polyethyleneimine-coated carbon nanotubes modified with methyl thioglycolate (CNTs-PEI-MTG) were prepared and used as adsorbent for Au(III) extraction. Carbon nanotubes (CNTs) were coated with polyethyleneimine (PEI), via physical adsorption. The PEI molecules were attached together using glutaraldehyde as crosslinking agent and subsequently modified with methyl thioglycolate (MTG) to improve the adsorption selectivity toward gold ions. The obtained products were characterized by scanning electron microscope equipped with energy dispersive X-ray analyzer (SEM-EDX), Fourier transforms infrared spectrometer (FTIR), Raman spectrometer (FT-Raman), thermal gravimetric analyzer (TGA), and ninhydrin test. The optimum amounts of PEI and MTG for CNTs coating were 2.00×10-4 and 17.86 mmol/g CNTs, respectively. The effect of certain parameters on Au(III) adsorption by the adsorbents was evaluated. Adsorption equilibrium was reached in 10 minutes. The solution pH in the range of 1.5-7 did not have any effect on the adsorption efficiency. The adsorption behavior followed Langmuir isotherm with the calculated maximum adsorption capacity of 203.7 mg Au/g. The desorption of Au(III) ions from the used adsorbent was achieved using 0.5 M thiourea in 2%(v/v) HCl solution.
DOI: 10.14457/cu.the.2011.218
title: Preparation of mixed-mode monolithic silica capillary column with octadecyl and aminopropyl groups for liquid chromatography

description: The silica monolith has been a substantial material used as stationary phase in chromatographic separation. Easy of preparation, versatile surface modification, better permeability and higher efficiency were the advantages of monolithic silica column comparing to silica packed column. In this work, The bared monolithic silica capillary column was first prepared via the sol-gel technique and followed by the post- modification with octadecylmethoxysilane and aminopropyltrimetho xysilane (C18-AP column). The chromatographic behaviors of the C18-AP column and monolithic silica capillary column modified with octadecyltrimethoxysilane (C18 column) were examined and compared according to Tanaka’s test. The hydrophobicity of the C18-AP column and the C18 column was similar while the shape selectivity of C18-AP column was better than C18 column. For ion exchange capacity and silanophilic interactions, C18-AP column showed repulsive electrostatic interactions due to the functionalized of aminopropyl groups on the stationary phase. Therefore, C18-AP column could exhibit a reversed phase and a weak anion exchange separation behavior. The separation performances of C18-AP column were then examined and compared to those of C18 column. The separation of alkylbenzenes and benzoic acid derivatives on both columns were similar which separated based on reversed phase mechanism. Inorganic anions and benzoate anions which were not separated on C18 column were successfully separated on C18-AP column because of a weak anion exchange characteristic. Moreover, the improvement of peak tailing problem for separation of basic compounds such as aniline derivatives on C18-AP column was observed. Lastly, the resolution of polyaromatic hydrocarbons (PAHs) separation on C18-AP column was better than C18 column due to the better shape selectivity of C18-AP column.
DOI: 10.14457/cu.the.2011.219
title: Thermoresponsive chitosan derivative for controlled release of fragrances

description: The amphiphilic thermoresponsive chitosan derivative was synthesized for using as a fragrance controlled release system. The obtained polymer could self-assemble in aqueous media to form polymeric particles with spherical shape. Poly(ethylene glycol), a hydrophilic polymer, was grafted onto chitosan backbone in order to act as thermoresponsive residue in which upon self-assembling became particles’ coronas, whereas perfumery aldehyde (in this case vanillin and citral) was grafted onto the chitosan to inherit hydrophobicity which upon self-assembling became particles’ core. The synthesized thermoresponsive polymer showed aggregation-dissociation behavior, corresponding with temperature trigger, leading to temperature dependence of the fragrance controlled release; significantly release of aldehyde from the particles was observed when the temperature was lower than lower critical solution temperature (LCST) whereas slower release was obvious at the temperature beyond the LCST. The LCST of system could be tuned by adjusting through salt concentration. This delivery system is completely biocompatible, therefore, it can be used in various applications.
DOI: 10.7910/dvn/oqgbgc/byx5z0
title: Jeehp_16_21_raw data.tab

description: :unav
DOI: 10.14457/cu.the.2011.220
title: Method development for determination of methylparaben, ethylparaben and propylparaben by ultra-performance liquid chromatography coupled with electrochemical detection

description: In this study, a new technique was developed using rapid ultra-performance liquid chromatography (UPLC)-based separation coupled with electrochemical detection by a boron-doped diamond (BDD) electrode for the detection and quantification of three commonly used parabens (methylparaben (MP), ethylparaben (EP) and propylparaben (PP)). The ultimate goal is aimed to reduce the analysis time by using UPLC coupled with a short reverse phase C18 monolithic column (25 mm x 4.6 mm). Operating the monolithic column at low back-pressure resulted in high flow rates. A mobile phase consisting of a 25:75 (v/v) ratio of acetonitrile: 0.05 M phosphate buffer (pH 5) at a flow rate of 2.5 mL min-1 was used to perform the separation. The amperometric detection with the BDD electrode was found to be optimal and reliably reproducible at a detection potential of 1.5 V vs. Ag/AgCl. Under these conditions, the separation of the three target analytes (MP, EP and PP) was achieved in 2 min and was linear within a sample concentration range of 0.1 to 50.0 mg L-1 (r2 &gt; 0.997). This method was successfully applied to determine the concentrations of each paraben in six real samples with the recoveries ranging from of 80.3 - 98.9% for all three parabens from samples spiked at 12, 22 and 32 mg L-1. Therefore, the proposed method can be used as an alternative rapid and selective method for the determination of paraben levels in real samples.
DOI: 10.14457/cu.the.2011.221
title: Investigating population and genetic structure in giant honey bee Apis dorsata Fabricius in Thailand

description: The giant honey bee (Apis dorsata) is an important pollinator of Asian lowland forests. Across its range, the species is impacted by heavy hunting and habitat disturbance. In this thesis, it was investigated how these pressures impact the connectivity and viability of the A. dorsata population of Thailand. In Chapter III, a morphometric analysis of forewing shape that can accurately identify any of the four species of honey bee present in Thailand was described, regardless of sex. Thus, geometric morphometry of the wing alone can be used to identify Asian honey bee species in most circumstances. In Chapter IV, the procedure to characterize 73 A. dorsata colonies collected from 31 different localities in Thailand was applied. Multivariate analysis of variance (MANOVA) demonstrated no significant differences between the bees sampled from five geographic regions. Therefore, this suggests that the A. dorsata populations of mainland Thailand are a single population. In Chapter VI, the genetic structure and colony relatedness of A. dorsata populations based on microsatellite analysis of 54 nests in 3 aggregations was examined. Also, it was shown that the population has high levels of heterozygosity and that FST values between aggregations were not significantly different from zero (P &gt; 0.05). The analysis also showed that no colonies were related as mother-daughter. Thus, if reproduction occurred at the study site, daughter colonies dispersed. This suggests that rapid increases in A. dorsata colony numbers during general flowering events most likely occur by swarms arriving from other areas rather than by in situ reproduction. In Chapter VII, queen mating frequency and allelic diversity between colonies sampled in disturbed and undisturbed areas in Thailand was compared. Microsatellite analysis of 18 colonies in 6 aggregations showed no significant difference in queen mating frequency at disturbed and undisturbed habitats. This suggests that the mating behaviour of A. dorsata is robust to anthropogenic changes to the landscape. It could be concluded that despite the formidable anthropogenic pressures that the A. dorsata population endures in Thailand, the species continues to enjoy a large effective population size and has high connectedness. Furthermore, this finding suggests that habitat disturbance has no effect on mating frequency or genetic diversity. It was concluded that A. dorsata is currently able to tolerate habitat fragmentation and annual harvesting.
DOI: 10.14457/cu.the.2011.222
title: Discrimination of nucleotides by supramolecular sensors in aqueous solution

description: This research aims to design and synthesize the molecular sensors for nucleotides sensing. In the case of the energy transfer, gold nanoparticles were used to study on the complexation related two processes including i) the complexation of nucleotides with the modified gold nanoparticles and fluorophore and ii) the fluorophore replacement on the gold nanoparticles by nucleotides. Unfortunately, the detection of nucleotides using the concepts of the fluorescence quenching by gold nanoparticles under both processes has not been achieved. For nucleotides discrimination using Principal Component Analysis (PCA), the molecular sensors containing pyrene and coumarin derivatives (Pydpa and Es) were used for discrimination of various nucleotides in aqueous solution. Initially, the complexation of Pydpa-Zn, Pydpa-Cu, Es-Zn, and Es-Cu were prepared as the sensory molecules to recognize the phosphate anion based nucleotides undergone the electrostatic interaction. The complexation studies of these sensors were carried out by fluorescence spectrophotometry. It was found that Pydpa-Zn, Es-Zn, and Es-Cu showed a high selectivity to PPi with the log K values of 8.83, 5.23 and 4.64, respectively. In addition, the PCA method was applied to classify the types of nucleotides by creation of the sensor arrays (the combinatorial data) and the mixed sensors (the combinatorial sensors). Interestingly, the combinatorial sensors of Pydpa-Zn+Es-Cu showed a highly potential discrimination of PPi, ATP, UTP, ADP, UDP and GDP with 92% classification accuracy.
DOI: 10.5281/zenodo.3385273
title: NGS data produced in 'Rapid selection and identification of functional CD8+ T-cell epitopes from large peptide-coding libraries'; Nature Communications (2019)

description: Sharma, G et al. Rapid selection and identification of functional CD8+ T-cell epitopes from large peptide-coding libraries. <em>Nature Communications</em>. Accepted (August 2019) <strong>Abstract:</strong> Cytotoxic CD8+ T-cells recognize and eliminate infected or malignant cells that present, at their cell surfaces, short peptide epitopes derived from intracellularly processed antigens. However, broadly searching for specific major histocompatibility complex (MHC)-bound peptide epitopes that are naturally processed and capable of eliciting a functional T-cell response has been challenging. Here, we report a method for deep and unbiased T-cell epitope profiling, which is done by using <em>in vitro</em> co-culture of CD8+ T-cells and target cells transduced with high-complexity epitope-encoding minigene libraries. Target cells that are subject to cytotoxic attack from T-cells in co-culture are isolated, before they are lost to apoptosis, by fluorescence-activated cell sorting and characterized by sequencing the minigenes encoded within. In the present study, we validate this highly parallelized method using known murine T-cell receptor/peptide-MHC pairs and diverse minigene-encoded epitope libraries to identify naturally processed and MHC-presented peptide epitopes unambiguously and with high sensitivity.
DOI: 10.5281/zenodo.3385274
title: NGS data produced in 'Rapid selection and identification of functional CD8+ T-cell epitopes from large peptide-coding libraries'; Nature Communications (2019)

description: Sharma, G et al. Rapid selection and identification of functional CD8+ T-cell epitopes from large peptide-coding libraries. <em>Nature Communications</em>. Accepted (August 2019) <strong>Abstract:</strong> Cytotoxic CD8+ T-cells recognize and eliminate infected or malignant cells that present, at their cell surfaces, short peptide epitopes derived from intracellularly processed antigens. However, broadly searching for specific major histocompatibility complex (MHC)-bound peptide epitopes that are naturally processed and capable of eliciting a functional T-cell response has been challenging. Here, we report a method for deep and unbiased T-cell epitope profiling, which is done by using <em>in vitro</em> co-culture of CD8+ T-cells and target cells transduced with high-complexity epitope-encoding minigene libraries. Target cells that are subject to cytotoxic attack from T-cells in co-culture are isolated, before they are lost to apoptosis, by fluorescence-activated cell sorting and characterized by sequencing the minigenes encoded within. In the present study, we validate this highly parallelized method using known murine T-cell receptor/peptide-MHC pairs and diverse minigene-encoded epitope libraries to identify naturally processed and MHC-presented peptide epitopes unambiguously and with high sensitivity.
DOI: 10.14457/cu.the.2011.223
title: Seasonal variation in emergence and activity pattern at roosting site of lyle's flying Pteropus lylei Andersen, 1908 at Pho, Chachoengsao province

description: This study addressed the question of how environmental factors influence the variation in emergence and daytime behavior of Lyle's flying fox, Pteropus lylei. Therefore, the colony-wide emergence, environmental factors and daytime behavior were investigated directly at roosting site of Lyle's flying fox (Wat Pho, Amphoe Bang Khla, Chachoengsao Province) for 12 months. It was found that the onset of colony-wide emergence time had significantly affected by sunset time and relative humidity. The duration of colony-wide emergence varied throughout the year, and tended to increase during parturition and early lactation periods. However, the duration was weakly correlated with seasonal change in twilight duration, and lunar phase had no influence on the colony-wide emergence of Lyle’s flying fox. The observation on individual emergence was conducted by filming. The results showed that males, female and juvenile tended to emergence later during reproductive periods, especially in parturition and early lactation. During that time, lactating females were the last to emerge. The later emergence of lactating females can be reflected from the enlargement of wing loading, which reduce flight performance and increase risk of predation. In conclusion, environmental factors and reproductive status play as crucial roles in emergence of Lyle's flying fox. From daytime behavior observations using scan-sampling technique, the most common behaviors performing by bats were sleeping, grooming and wing flapping. Variation in sleeping, grooming, wing flapping and mating/courtship between times of day and times of the year were apparently observed. Furthermore, wing spreading, movement and mating/courtship were observed in males more frequently than in females, whereas females exhibited grooming and aggressive behaviors more frequently than males. In conclusion, time of day, season and sex have influence on daytime behavior of Lyle's flying fox.
DOI: 10.14457/cu.the.2011.224
title: Thermoresponsive polymer-stabilized gold nanoparticles for protein detection

description: Gold nanoparticles stabilized by thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM-AuNPs), were prepared by surface grafting of thiol-terminated PNIPAM (having two targeted molecular weight 6 and 8 kDa) onto citrate-stabilized AuNPs. The color change of the PNIPAM-AuNPs solution from red to blue-purple without precipitation when the solution was heated to 40 ºC, above the lower critical solution temperature (LCST) of PNIPAM indicated the thermoresponsive property of the synthesized AuNPs. In this work, PNIPAM-AuNPs were used to detect proteins by chemical nose approach based on fluorescence quenching of fluorophore by AuNPs. In the detection, upon an addition of protein analyte, quenched fluorophore absorbed on the AuNPs surface can be attracted by protein and released from the AuNPs surface resulting in the recovery of fluorescence signal of the fluorophore. An array-based sensing platform for detection of six proteins can be developed from the PNIPAM-AuNPs having different molecular weight and conformation in combination with a G0 dendritic phenylene-ethynylene fluorophore having positively charged N(CH3)3+ groups. From Linear Discriminant Analysis (LDA), it was found that 100% accuracy of protein classification can be achived by using only two types of PNIPAM-AuNPs.
DOI: 10.14457/cu.the.2011.225
title: Antiproliferative activity against cancer cells of apis mellifera propolis from nan province, Thailand

description: Propolis is one of honeybee products. It provides a lot of bioactivities such as antibacterial, antifungal, anti-inflammatory, anti-tumor, etc. In this research, propolis of Apis mellifera was focused. It was collected from Nan province, Thailand. Propolis was initially extracted by 96% (v/v) EtOH and water. The antiproliferative activity of crude extract was performed against 5 cancer cell lines which were human duetol carcinoma (BT474), human lung undifferentiated (Chago), human liver hepatoblastoma (Hep-G2), human gastric carcinoma (KATO-III), and human colon adenocarcinoma (SW620). Also, fibroblast cells (Hs27) were used as control. It was found that, by MTT assay, crude EtOH extract of propolis (CEE) had the good antiproliferative activity against those selected cells. Later, CEE was partitioned by hexane, CH2Cl2, and MeOH in order to separate compounds based on their polarities. Each part was tested against those selected cells. The result indicated that crude hexane extract of propolis (CHE) had the best antiproliferative activity against cancer cells. After that, CHE was further purified by various chromatographies. All obtained fractions were tested against those selected cells by MTT assay again. It was presented that fractions 3 (compound 1) and 5 (compound 2) had the good antiproliferative activity. The value of inhibition concentration at 50% (IC50) of compound 1 for SW620, Hep-G2, BT474, KATO-III, Chago, and Hs27 were 10.76, 21.52, 13.95, 10.93, 29.30, and 21.35 µg/ml, respectively. The IC50 values of compound 2 for SW620, Hep-G2, KATO-III, Chago, BT474, and Hs27 were &lt;3.125, 5.97, 4.03, 5.78, 4.41, and 5.97 µg/ml, respectively. After being analyzed by Nuclear Magnetic Resonance and Mass Spectroscopy, it was shown that compound 1 was in a cardanol group and compound 2 was in a cardol group.
DOI: 10.14457/cu.the.2011.226
title: Semi-interpenetration of Poly(ethylene glycol) into Poly(L-lactic acid) network

description: Radical crosslinking of methacrylate-endcapped PLLA (PLLA-Meth) with PEG dispersed in the crosslinked PLLA network was carried out in order to prepare hydrophilic PLLA/PEG semi-interpenetrating network (semi-IPN). PLLA was first prepared by direct condensation polymerization from L-lactic acid. The number average molecular weight of PLLA was around 3,000-5,000 Da. PLLA with one or two methacrylate end groups were prepared by reacting PLLA or PLLA-diol chain with methacrylic anhydride. The degree of substitution of methacrylate on PLLA as determined by 1H NMR was over 90% when 40 equivalents (relative to PLLA chains) of methacrylate was added in the reaction. The gel content of thermal cure using benzoyl peroxide as an initiator was 62% while no crosslinking occurred by 2-hydroxy-2-methylpropiophenone initiator induced by UV. The PLLA/PEG semi-IPN was carried out by heating the PLLA-Meth mixed with PEG (Mw = 20,000 Da) at 150 °C in the presence of 10% of benzoyl peroxide. Water uptake of the prepared PLLA/PEG semi-IPN was as high as 9% for the composite having 40% (by weight) of PEG.
DOI: 10.14457/cu.the.2011.227
title: Hollow fiber supported liquid membrane for inorganic arsenic speciation

description: Hollow fiber supported liquid membrane (HF-SLM) was developed for speciation and preconcentration of inorganic arsenic in drinking water. The three-phase extraction mode was applied, where the sample solution was a donor phase; the Aliquat 336 in kerosene impregnated in the pores of the membrane was an extracting phase; and NaOH was an acceptor phase. The sample solution was adjusted to pH 6, so As(V) was in anion form (H2AsO4-) while As(III) was in neutral form (H3AsO3). The As(V) in the donor phase was extracted into the acceptor phase via anion-exchange mechanism. After extraction, As(V) was determined by the flow-based molybdenum blue method. For the determination of As(III), As(III) was oxidized to As(V) by KMnO4 and determined as total As. Then the amount of As(III) was obtained by subtracting of As(V) from the total amount of As. The method was applied for speciation of inorganic arsenic in drinking water from local market. The enrichment factor more than 250 was obtained. The linear range was 30-110 μg L-1, which provided the correlation coefficients (R2) of more than 0.99. The detection limit was about 5 µg L-1. The recoveries for determination of As(III) and As(V) were 86 to 102% and 92 to 112%, respectively and relative standard deviations of As(III) and As(V) were less than 5%. The method was compared with the ICP-AES method for the determination of total As. The concentration of total As from both methods were not significantly different (P &gt; 0.05, Paired t-Test). This method is simple, low cost, uses less solvent and can be used for determination of inorganic arsenic at trace level.
DOI: 10.14457/cu.the.2011.228
title: Structural effects of bispyrenyl ureas linked by polyethylene glycol and ethyne spacers towoed anion binding abilities

description: Anion sensors possessing bispyrenyl derivatives have been designed and synthesized. Compound L1 containing amide groups for binding anions and benzyl ring as spacer was synthesized in 41% yield. Compound L2 containing thiourea groups as anion binding sites and polyethyleneglycol chains linked with benzyl ring as spacer was synthesized in 51% yield. Compound L3 containing urea as anion binding sites and ethyne as spacer could not be synthesized. Therefore, we have studied the anion abilities of L1 and L2 using fluorescence spectrophotometry. Compound L1 did not interact with any anions as suggested by an insignificant change of the emission spectra. Emission spectra of compound L2 changed from monomer band (410 nm) to excimer band (515 nm) upon addition of anions. The order of intensity ratio between excimer and monomer bands (IE/IM) in L2 varied as F- &gt; OH- &gt;&gt; AcO- &gt; BzO- &gt; H2PO4- &gt; Cl- &gt; Br-≈ I-. Due to a smaller size and more basicity of F-, it could form H-bonding interactions with protons of N-H thiourea of L2. A large amount of F- and OH- induced the deprotonation of N-H thiourea while AcO- formed H-bonding interactions only. Complexation of L2 and F- occurred via intermolecular pyrene stacking while that of L2 and AcO- as well as OH- occurred via intramolecular pyrene stacking.