cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20522907.v1
title: Delivery of active minocycline hydrochloride by local sustained-release system of complex and thermoresponsive hydrogel for dogs

description: ABSTRACT The objective of this study was to develop a novel subgingival sustained-release system for local delivery of bioactive minocycline hydrochloride for periodontal disease treatment in dogs. The system incorporated the Minocycline hydrochloride-Calcium-Dextran sulfate sodium into a thermoresponsive Pluronic F127 hydrogel. Minocycline hydrochloride was sustained release from the system for up to 10 days and the release kinetics fit the power law model. The release medium had a significant statistical difference in antimicrobial activity to Aggregatibacter actinomycetemcomitans. The results showed the system was a promising subgingival sustained-release minocycline hydrochloride delivery system for periodontal disease treatment in dogs.
DOI: 10.6084/m9.figshare.6891305.v1
title: MOESM12 of CRX directs photoreceptor differentiation by accelerating chromatin remodeling at specific target sites

description: Additional file 12. De novo motif analysis identifies sequences enriched in different sets of CRX-bound regions. Data presented in columns A–C & E–I are generated by HOMER (see “Methods”). Column D represents the scanning of the identified sequence back across the 6 classes of CRX sites (+/− 1kb from peak center). The plot in line 1 depicts the layout of the plot, and the y axis quantifies ‘motifs per bp.’
DOI: 10.5281/zenodo.10302622
title: Data in support of the article by Guerrieri et al. published in Nature Geoscience

description: Data shared here were used in the four main Figures, Extended Data Figures and Supplementary Figures showed in the article 'Substantial contribution of tree canopy nitrifiers to nitrogen fluxes in European forests' by Rossella Guerrieri, Joan Cáliz, Stefania Mattana, Anna Barceló, Marco Candela, David Elustondo, Heike Fortmann, Sofie Hellsten, Nils Koenig, Antti-Jussi Lindroos, Giorgio Matteucci, Päivi Merilä, Greg Michalski, Manuel Nicolas, Anne Thimonier, Silvia Turroni, Elena Vanguelova, Arne Verstraeten, Peter Waldner, Mirai Watanabe, Emilio O. Casamayor, Josep Peñuelas, Maurizio Mencuccini, published in Nature Geoscience (DOI 10.1038/s41561-023-01364-3).

Data is provided as Excel files. For methodological information, please refer to the article.
DOI: 10.5281/zenodo.10302621
title: Data in support of the article by Guerrieri et al. published in Nature Geoscience

description: Data shared here were used in the four main Figures, Extended Data Figures and Supplementary Figures showed in the article 'Substantial contribution of tree canopy nitrifiers to nitrogen fluxes in European forests' by Rossella Guerrieri, Joan Cáliz, Stefania Mattana, Anna Barceló, Marco Candela, David Elustondo, Heike Fortmann, Sofie Hellsten, Nils Koenig, Antti-Jussi Lindroos, Giorgio Matteucci, Päivi Merilä, Greg Michalski, Manuel Nicolas, Anne Thimonier, Silvia Turroni, Elena Vanguelova, Arne Verstraeten, Peter Waldner, Mirai Watanabe, Emilio O. Casamayor, Josep Peñuelas, Maurizio Mencuccini, published in Nature Geoscience (DOI 10.1038/s41561-023-01364-3).

Data is provided as Excel files. For methodological information, please refer to the article.
DOI: 10.6084/m9.figshare.22619947.v1
title: Additional file 2 of circACTR2 attenuates gemcitabine chemoresiatance in pancreatic cancer through PTEN mediated PI3K/AKT signaling pathway

description: Supplementary Material 2: Table S2. List of predicted miRNA targets of circACTR2 based on TargetScan & miRanda in this study
DOI: 10.6084/m9.figshare.22619947
title: Additional file 2 of circACTR2 attenuates gemcitabine chemoresiatance in pancreatic cancer through PTEN mediated PI3K/AKT signaling pathway

description: Supplementary Material 2: Table S2. List of predicted miRNA targets of circACTR2 based on TargetScan & miRanda in this study
DOI: 10.6084/m9.figshare.14708104.v1
title: Additional file 3 of A designathon to co-create community-driven HIV self-testing services for Nigerian youth: findings from a participatory event

description: Additional file 3: Supplement 3. Judges' scores for all teams at the designathon: Nigeria 2019*.
DOI: 10.25345/c5gx4555d
title: MassIVE MSV000094038 - LC-IMS-MS test dataset of 32 substances
title: LC IMS MS dataset of 32 standard compounds. Compounds were prepared in acn/water 1:1 and analysed using a ultimate 3000 and a waters acquity c18 beh column. Gradient elution started at 5% B and was increased to 95% B at 10 min, held until 14min, decreased to 5% at 14.01 min and held until 17.5 min. The flow rate was 0.4 mL/min.
DOI: 10.6084/m9.figshare.20521379
title: Additional file 2 of Comparative transcriptome analysis of rice cultivars resistant and susceptible to Rhizoctonia solani AG1-IA

description: Additional file 2. Number of expressed genes in all samples (FPKM&gt;0.5).
DOI: 10.6084/m9.figshare.12134277
title: Additional file 6 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 6. List of 37 candidate signature piRNAs captured from 44 candidate signature after sex related gene ontology (GO) enrichment and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway enrichment analysis.
DOI: 10.6084/m9.figshare.12134280
title: Additional file 7 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 7. The expression profiles of 37 signature piRNAs by small RNA sequencing in males and pseudomales
DOI: 10.6084/m9.figshare.12134274
title: Additional file 5 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 5. List of Target genes of the 44 candidate signature piRNAs that differentially expressed in males and pseudo males.
DOI: 10.6084/m9.figshare.12134274.v1
title: Additional file 5 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 5. List of Target genes of the 44 candidate signature piRNAs that differentially expressed in males and pseudo males.
DOI: 10.6084/m9.figshare.20522907
title: Delivery of active minocycline hydrochloride by local sustained-release system of complex and thermoresponsive hydrogel for dogs

description: ABSTRACT The objective of this study was to develop a novel subgingival sustained-release system for local delivery of bioactive minocycline hydrochloride for periodontal disease treatment in dogs. The system incorporated the Minocycline hydrochloride-Calcium-Dextran sulfate sodium into a thermoresponsive Pluronic F127 hydrogel. Minocycline hydrochloride was sustained release from the system for up to 10 days and the release kinetics fit the power law model. The release medium had a significant statistical difference in antimicrobial activity to Aggregatibacter actinomycetemcomitans. The results showed the system was a promising subgingival sustained-release minocycline hydrochloride delivery system for periodontal disease treatment in dogs.
DOI: 10.6084/m9.figshare.12134283
title: Additional file 8 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 8. List of 53 DNA methylation related target genes identified that predicted to interact with 15 regulating piRNAs
DOI: 10.25345/c5c53fc1k
title: MassIVE MSV000094039 - LC-MS test dataset of 32 substances
title: LC MS dataset of 32 standard compounds. Compounds were prepared in acn/water 1:1 and analysed using a ultimate 3000 and a waters acquity c18 beh column. Gradient elution started at 5% B and was increased to 95% B at 10 min, held until 14min, decreased to 5% at 14.01 min and held until 17.5 min. The flow rate was 0.4 mL/min.
DOI: 10.6084/m9.figshare.20521407.v1
title: Additional file 2 of Weighted single-step GWAS and RNA sequencing reveals key candidate genes associated with physiological indicators of heat stress in Holstein cattle

description: Additional file 2: Table S1. Differential gene expression between non-heat stress group and heat stress group.
DOI: 10.6084/m9.figshare.12134280.v1
title: Additional file 7 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 7. The expression profiles of 37 signature piRNAs by small RNA sequencing in males and pseudomales
DOI: 10.6084/m9.figshare.6891470.v1
title: MOESM2 of Regulatory network of circRNA–miRNA–mRNA contributes to the histological classification and disease progression in gastric cancer

description: Additional file 2: Fig. S1. Prediction of circHIPK3- miR-124/miR-29b pathway. (A) Statistically significant correlations were revealed between miR-29b or miR-124 and their mediated pathways by P-value (log scaled) in heatmap. Red represents high significance. (B) The relevant pathways of miR-29b mediated, miR-124 mediated, and miR-29b and miR-124 jointly mediated. (C) The magnitudes of the significant correlation about Pathways among miR-29b mediated, miR-124 mediated, and miR-29b and miR-124 jointly mediated by P-value (−log2 scaled).
DOI: 10.6084/m9.figshare.12134277.v1
title: Additional file 6 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 6. List of 37 candidate signature piRNAs captured from 44 candidate signature after sex related gene ontology (GO) enrichment and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway enrichment analysis.
DOI: 10.6084/m9.figshare.12134283.v1
title: Additional file 8 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 8. List of 53 DNA methylation related target genes identified that predicted to interact with 15 regulating piRNAs
DOI: 10.6084/m9.figshare.20521376.v1
title: Additional file 1 of Comparative transcriptome analysis of rice cultivars resistant and susceptible to Rhizoctonia solani AG1-IA

description: Additionalfile 1: Figure S1. Phenotype identification of sheath blight diseasedetected in Gangyuan8 and Yanfeng47. FigureS2. The relative area of disease spot in different inoculation time point. Figure S3. The overall relatedness oftranscriptomes of different times. FigureS4. Assess the similarity between samples across conditions by PCA analyse.Figure S5. Functional classificationof different express genes (DEGs) in the Gangyuan8 (GG) and Yanfeng47 (YY). Figure S6. GO enrichment analysis ofdifferentially expressed genes. Figure S7.GO function enrichment analysis of clusters. Figure S8. KEGG enrichment analysis of differentially expressedgenes. Figure S9. Verification ofdifferentially expressed genes by qRT-PCR. Supplementary Table S1. Statistics on the number of differentially expressedgenes. Supplementary Table S2.Specific primers of differential gene sequences for qRT-PCR.
DOI: 10.6084/m9.figshare.12134286.v1
title: Additional file 9 of Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

description: Additional file 9. List of 16 target genes related to transposition, especially heterochromatin formation identified together with their 10 interacting piRNAs.
DOI: 10.6084/m9.figshare.6891470
title: MOESM2 of Regulatory network of circRNA–miRNA–mRNA contributes to the histological classification and disease progression in gastric cancer

description: Additional file 2: Fig. S1. Prediction of circHIPK3- miR-124/miR-29b pathway. (A) Statistically significant correlations were revealed between miR-29b or miR-124 and their mediated pathways by P-value (log scaled) in heatmap. Red represents high significance. (B) The relevant pathways of miR-29b mediated, miR-124 mediated, and miR-29b and miR-124 jointly mediated. (C) The magnitudes of the significant correlation about Pathways among miR-29b mediated, miR-124 mediated, and miR-29b and miR-124 jointly mediated by P-value (−log2 scaled).
DOI: 10.6084/m9.figshare.20523012.v1
title: Circular RNA circENTPD7 suppresses the accumulation of PTEN to promote cell proliferation in non-small cell lung cancer

description: Abstract The oncogenic role of circular RNA ENTPD7 (circENTPD7) in cancer biology has been reported in glioblastoma, while its role in non-small cell lung cancer (NSCLC) is unknown. This study was performed to investigate the involvement of circENTPD7 in NSCLC. NSCLC tissues and paired non-tumor tissues were collected from 64 NSCLC patients and the expression of circENTPD7 and PTEN were determined by RT-qPCR. Expression levels of PTEN protein in these tissue samples were measured by ELISA. The 64 NSCLC patients were subjected to a follow-up study to explore the role of circENTPD7 in predicting the survival of NSCLC. Overexpression of circENTPD7 was achieved in NSCLC cells, and the effects of overexpression of circENTPD7 on the expression of PTEN were measured by RT-qPCR and Western blot at mRNA and protein level, respectively. Cell proliferation was assessed by CCK-8 assay. CircENTPD7 was upregulated in NSCLC and high expression levels of circENTPD7 predicts the poor survival rate of NSCLC cells. In NSCLC tissues, circENTPD7 was inversely correlated with PTEN protein but not mRNA. In NSCLC tissues, overexpression of circENTPD7 resulted in downregulation of PTEN, but did not alter the expression of PTEN mRNA. Cell proliferation analysis showed that overexpression of circENTPD7 promoted the proliferation of NSCLC cells and reduced the inhibitory effects of overexpression of PTEN on cell proliferation. CircENTPD7 may suppress the accumulation of PTEN to promote cell proliferation in NSCLC.
DOI: 10.6084/m9.figshare.20523012
title: Circular RNA circENTPD7 suppresses the accumulation of PTEN to promote cell proliferation in non-small cell lung cancer

description: Abstract The oncogenic role of circular RNA ENTPD7 (circENTPD7) in cancer biology has been reported in glioblastoma, while its role in non-small cell lung cancer (NSCLC) is unknown. This study was performed to investigate the involvement of circENTPD7 in NSCLC. NSCLC tissues and paired non-tumor tissues were collected from 64 NSCLC patients and the expression of circENTPD7 and PTEN were determined by RT-qPCR. Expression levels of PTEN protein in these tissue samples were measured by ELISA. The 64 NSCLC patients were subjected to a follow-up study to explore the role of circENTPD7 in predicting the survival of NSCLC. Overexpression of circENTPD7 was achieved in NSCLC cells, and the effects of overexpression of circENTPD7 on the expression of PTEN were measured by RT-qPCR and Western blot at mRNA and protein level, respectively. Cell proliferation was assessed by CCK-8 assay. CircENTPD7 was upregulated in NSCLC and high expression levels of circENTPD7 predicts the poor survival rate of NSCLC cells. In NSCLC tissues, circENTPD7 was inversely correlated with PTEN protein but not mRNA. In NSCLC tissues, overexpression of circENTPD7 resulted in downregulation of PTEN, but did not alter the expression of PTEN mRNA. Cell proliferation analysis showed that overexpression of circENTPD7 promoted the proliferation of NSCLC cells and reduced the inhibitory effects of overexpression of PTEN on cell proliferation. CircENTPD7 may suppress the accumulation of PTEN to promote cell proliferation in NSCLC.
DOI: 10.57932/5223fd82-10ce-490c-a4b0-1106b5511554
title: Year-round daily Meteopole-Flux weather, energy, carbon and soil-plant variables

description: Since 2012, Meteo-France and CNRM have been measuring meteorological variables, energy, carbon and water fluxes, as well as soil moisture and soil temperature at various depths at its Meteopole site in Toulouse. This is a daily subset from 2016 to 2022 of the full half-hourly database available on the ACTRIS and ICOS (FR-Tou site) portals, including infrared land surface temperature and leaf area index derived from a sunphotometer. In this subset, the observations are close to solar noon.
DOI: 10.24377/ljmu.d.00000175
title: “It's a big added stress on top of being so ill”: The challenges facing people prescribed cannabis in the UK

description: A short animated film based on the real stories of UK medical cannabis patients. Developed from qualitative academic research by Dr Lindsey Metcalf McGrath & Dr Helen Beckett Wilson, LJMU.
description: Qualitative Interviews
DOI: 10.6084/m9.figshare.22620187
title: Additional file 5 of Human menstrual blood-derived stem cells reverse sorafenib resistance in hepatocellular carcinoma cells through the hyperactivation of mitophagy

description: Additional file 5. Test information and values supporting the statistical graphs.
DOI: 10.17630/1640cfe0-43b2-4221-b997-8497d5e055bf
title: Molecular analysis of the eggshell and cuticle surface of the potato cyst nematode, Globodera rostochiensis (dataset)

description: Plant-parasitic nematodes regulate their interactions with the external environment and their host. The surface of the nematodes, whether the cuticle or the eggshell, plays a key role in this process. Work undertaken in this project aimed to exploit new genome resources for potato cyst nematodes (PCN) to allow characterisation of these structures. The eggshell is a key survival structure for PCN. Hatching of juvenile potato cyst nematodes occurs in response to host derived hatching factors. Little is known about the molecular mechanisms that underpin hatching in response to these host diffusates and nothing is known about the molecular composition of the PCN eggshell. Methods that allow extraction of proteins and lipids from large numbers of isolated PCN eggshells were developed. These extraction techniques permitted identification of a range of proteins present in the eggshell, including chondroitin proteoglycans and a calcium dependent phospholipid-binding annexin. The annexin was focused on due to the apparent major role of calcium in PCN hatching. This annexin was localised to the eggshells of Globodera rostochiensis by immunofluorescence. To our knowledge, this makes the annexin the first eggshell protein to be identified and localised in any plant-parasitic nematode. The annexin was shown to bind to phospholipids and RNAi of the annexin showed that reducing expression of the eggshell annexin alters nematode hatching patterns in response to host hatching factors. The eggshell permeability barrier is essential for the development of a juvenile within the embryo. Eggshell lipid analysis has given insight into components of the permeability barrier. Identified lipid species highlight potential function within the nematode hatching cascade through calcium interaction or down stream signalling. Attempts to identify the presence of nematode specific glycolipids, ascarosides, within the PCN eggshell showed that ascarosides or ascarylose were not detectable within PCN eggs, cysts or juveniles. It has previously been shown that the infective juveniles are able to alter their surface composition to avoid damage from host defence mechanisms. Here, methods that allow labelling of proteins present on the cuticle surface of PCN were developed. Three proteins; Galectin-5, Cri-2 and a nematode specific hypothetical protein that are present on the surface of PCN second stage juveniles were further investigated. These proteins were tested for their potential to suppress host defences through a variety of protein-small molecule interactions. This analysis showed that surface coat associated galectins could offer protection to an invasive juvenile through sequestering cell wall breakdown products, silencing detection of the pathogen.
DOI: 10.6084/m9.figshare.22620187.v1
title: Additional file 5 of Human menstrual blood-derived stem cells reverse sorafenib resistance in hepatocellular carcinoma cells through the hyperactivation of mitophagy

description: Additional file 5. Test information and values supporting the statistical graphs.
DOI: 10.17630/84c3a74e-eb89-4b37-a3ed-cd1cf0feeae3
title: Asymmetric base pair opening drives helicase unwinding dynamics (dataset)

description: The opening of a Watson-Crick double helix is required for crucial cellular processes, including replication, repair and transcription. It has long been assumed that RNA or DNA base pairs are broken by the concerted symmetric movement of complementary nucleobases. By analyzing thousands of base-pair opening and closing events from molecular simulations, here we uncover a systematic stepwiseprocessdrivenbytheasymmetricﬂipping-outprobability of paired nucleobases. We demonstrate experimentally that such asymmetry strongly biases the unwinding efﬁciency of DNA helicases towards substrates that bear highly-dynamic nucleobases, such as pyrimidines, on the displaced strand. Duplex substrates with identical thermodynamic stability are thus shown to be more easilyunwoundfromonesidethantheother,inaquantiﬁableand predictable manner. Our results indicate a possible layer of gene regulation coded in the direction-dependent unwindability of the double helix.
DOI: 10.6084/m9.figshare.6891629
title: Additional file 1: of Three-dimensional printing models in congenital heart disease education for medical students: a controlled comparative study

description: Questionnaire and quiz scores. (XLSX 13 kb)
DOI: 10.17630/330d75dc-5fee-4728-921e-8c2af53ccb6a
title: Nonlinear Mach-Zehnder interferometer with ultrabroadband squeezed light (dataset)
DOI: 10.17630/289e627f-5177-4b34-8c62-976384401ae0
title: Diode-pumped Tm3+-doped sesquioxide lasers for ultrashort pulse applications in the 2µm region (thesis data)

description: The processed experimental data presented in the thesis as titled above is provided in .txt file formats. Importing the files into widely available data analysis packages, e.g., Excel, as tab separated values should allow for any necessary analysis.
DOI: 10.17630/2b3d2f0e-7add-46e0-9b2e-db94fbbbc0e1
title: Structure and dynamics of iron pentacarbonyl (dataset)
DOI: 10.6084/m9.figshare.6891629.v1
title: Additional file 1: of Three-dimensional printing models in congenital heart disease education for medical students: a controlled comparative study

description: Questionnaire and quiz scores. (XLSX 13 kb)