cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21856131.v1
title: Effect of preparation methods on tosufloxacin tosylate/ hydroxypropyl-β-cyclodextrin inclusion complex

description: Abstract The main purpose of this work was to compare the effects of the four preparation methods on the TFLX/HP-β-CD inclusion complex. The effects of different preparation methods on the inclusion complex were investigated by SEM, DSC, PXRD, FT-IR and 1H NMR. All the characterization information indicated that the four preparation methods could cause interaction between TFLX and HP-β-CD, but the inclusion complex prepared by solvent evaporation has more reaction sites. Phase solubility experiments demonstrated that the inclusion reaction was spontaneous. In vitro dissolution experiments showed that the dissolution of the inclusion complex in water was: solvent evaporation method (64.39%) &gt; grinding method (42.37%) &gt; ultrasonic method (40.00%) &gt; freezing method (36.08%), and all higher than pure TFLX and physical mixture. These results suggest that the solvent evaporation is the most suitable method for preparing TFLX/HP-β-CD inclusion complexes.
DOI: 10.6084/m9.figshare.21856162
title: Pharmacokinetics and pharmacodynamics of Astragali Radix-Corni Fructus Herb-pair Extract, in kidney-yin deficiency model

description: Abstract To investigate the pharmacokinetics and pharmacodynamics of Astragali Radix-Corni Fructus herb-pair in kidney-yin deficiency model, which was made by continuously oral gavage of thyroxine. A simple and rapid LC-MS/MS method was developed and validated for the determination of loganin and morroniside in rat plasma and used for the pharmacokinetics study. The kidney-yin deficiency significantly changed the AUC(0-∞), Cmax and CLz/F of loganin and morroniside. The T1/2z of morroniside increased significantly in the kidney-yin deficiency rats. For the pharmacodynamics study, the liver index, kidney index, and ALT, TBIL, UREA, CREA level in the kidney-yin deficiency mice were examined. The herb-pair had been proved to be effective for the treatment of kidney-yin deficiency by affecting the liver, kidney, ALT, UREA and CREA, which showed positively correlated with the dose. The pharmacokinetics and pharmacodynamics studies in the pathological status could offer more valuable information for the future application of Astragali Radix-Corni Fructus herb-pair.
DOI: 10.6084/m9.figshare.16613337.v1
title: Long noncoding RNA SATB1-AS1 contributes to the chemotherapy resistance through the microRNA-580/ 2’-5’-oligoadenylate synthetase 2 axis in acute myeloid leukemia

description: Acute myeloid leukemia (AML) represents a hematopoietic cancer with an invasive property. Chemoresistance blunts the therapeutic effect of chemotherapeutics in AML. Long noncoding RNAs (lncRNAs) have been implicated in chemotherapy resistance in AML. Transcriptome sequencing in the current study was applied to clarify the differentially expressed lncRNAs between peripheral blood mononuclear cells of AML and normal samples. The expression of special AT-rich sequence binding protein 1 antisense RNA 1 (SATB1-AS1) and 2ʹ-5ʹ-oligoadenylate synthetase 2 (OAS2) in AML patients was evaluated by qRT-PCR. The relationships among SATB1-AS1, microRNA-580 (miR-580) and OAS2 were investigated by dual-luciferase reporter assay. We observed that SATB1-AS1 and OAS2 were upregulated, while miR-580 was downregulated in AML patients. SATB1-AS1 depletion suppressed proliferation, and enhanced apoptosis and sensitivity of AML cells. Additionally, SATB1-AS1 promoted the expression of OAS2 by acting as a molecular sponge of miR-580 in AML. miR-580 downregulation, OAS2 overexpression and a selective glycogen synthase kinase (GSK)-3β inhibitor AR-A014418 abolished the effects of SATB1-AS1 deletion on the chemosensitivity of AML cells. In conclusion, SATB1-AS1 knockdown promotes the sensitivity of AML cells by upregulating miR-580 and downregulating OAS2 through the GSK3β/β-catenin pathway, providing new insights into the function of SATB1-AS1 as a miRNA sponge in AML.
DOI: 10.6084/m9.figshare.25249291
title: Mineral analysis of developing fetal humerus bone- EDS data

description: The study aimed to analyze the mineral composition of the developing humerus bone at different trimesters of pregnancy
DOI: 10.6084/m9.figshare.25249291.v1
title: Mineral analysis of developing fetal humerus bone- EDS data

description: The study aimed to analyze the mineral composition of the developing humerus bone at different trimesters of pregnancy
DOI: 10.6084/m9.figshare.13466949
title: Additional file 5 of Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus

description: Additional file 5: Figure S3. The negative controls of MIP, FRWa, FxFa and FILa in U. unicinctus larvae detected by whole-mount in situ hybridization.
DOI: 10.6084/m9.figshare.16613337
title: Long noncoding RNA SATB1-AS1 contributes to the chemotherapy resistance through the microRNA-580/ 2’-5’-oligoadenylate synthetase 2 axis in acute myeloid leukemia

description: Acute myeloid leukemia (AML) represents a hematopoietic cancer with an invasive property. Chemoresistance blunts the therapeutic effect of chemotherapeutics in AML. Long noncoding RNAs (lncRNAs) have been implicated in chemotherapy resistance in AML. Transcriptome sequencing in the current study was applied to clarify the differentially expressed lncRNAs between peripheral blood mononuclear cells of AML and normal samples. The expression of special AT-rich sequence binding protein 1 antisense RNA 1 (SATB1-AS1) and 2ʹ-5ʹ-oligoadenylate synthetase 2 (OAS2) in AML patients was evaluated by qRT-PCR. The relationships among SATB1-AS1, microRNA-580 (miR-580) and OAS2 were investigated by dual-luciferase reporter assay. We observed that SATB1-AS1 and OAS2 were upregulated, while miR-580 was downregulated in AML patients. SATB1-AS1 depletion suppressed proliferation, and enhanced apoptosis and sensitivity of AML cells. Additionally, SATB1-AS1 promoted the expression of OAS2 by acting as a molecular sponge of miR-580 in AML. miR-580 downregulation, OAS2 overexpression and a selective glycogen synthase kinase (GSK)-3β inhibitor AR-A014418 abolished the effects of SATB1-AS1 deletion on the chemosensitivity of AML cells. In conclusion, SATB1-AS1 knockdown promotes the sensitivity of AML cells by upregulating miR-580 and downregulating OAS2 through the GSK3β/β-catenin pathway, providing new insights into the function of SATB1-AS1 as a miRNA sponge in AML.
DOI: 10.6084/m9.figshare.21856162.v1
title: Pharmacokinetics and pharmacodynamics of Astragali Radix-Corni Fructus Herb-pair Extract, in kidney-yin deficiency model

description: Abstract To investigate the pharmacokinetics and pharmacodynamics of Astragali Radix-Corni Fructus herb-pair in kidney-yin deficiency model, which was made by continuously oral gavage of thyroxine. A simple and rapid LC-MS/MS method was developed and validated for the determination of loganin and morroniside in rat plasma and used for the pharmacokinetics study. The kidney-yin deficiency significantly changed the AUC(0-∞), Cmax and CLz/F of loganin and morroniside. The T1/2z of morroniside increased significantly in the kidney-yin deficiency rats. For the pharmacodynamics study, the liver index, kidney index, and ALT, TBIL, UREA, CREA level in the kidney-yin deficiency mice were examined. The herb-pair had been proved to be effective for the treatment of kidney-yin deficiency by affecting the liver, kidney, ALT, UREA and CREA, which showed positively correlated with the dose. The pharmacokinetics and pharmacodynamics studies in the pathological status could offer more valuable information for the future application of Astragali Radix-Corni Fructus herb-pair.
DOI: 10.6084/m9.figshare.12943339.v1
title: Anatomy of a new specimen of <i>Piscivorenantiornis inusitatus</i> (Aves: Enantiornithes) from the Lower Cretaceous Jehol Biota

description: We describe a new enantiornithine specimen from the Lower Cretaceous Jiufotang Formation of the Jehol Biota, northeastern China, which can be assigned to the species <i>Piscivorenantiornis inusitatus</i>. The new specimen confirms the presence of the unusual articulation of the cervical vertebrae that characterizes <i>P. inusitatus</i>. The disarticulated bony elements in the new specimen, some of which are preserved in different views or completely missing from the holotype, enable a more comprehensive morphological reconstruction and a refined phylogenetic assessment of <i>P. inusitatus</i>. The new fossil demonstrates that the occipitals are recessed by a pair of fossae lateral to the foramen magnum, a feature otherwise unknown among Mesozoic and extant birds. Our phylogenetic analysis tentatively resolves <i>P. inusitatus</i> as the sister taxon to the clade consisting of <i>Pterygornis dapingfangensis</i> and <i>Mirusavis parvus</i>, which is recovered deeply nested within the Enantiornithes. We hypothesize that the craniolateral processes of the sternum are a derived feature in enantiornithines.
DOI: 10.6084/m9.figshare.13467599.v1
title: Additional file 2 of Mitochondrial dysfunction and consequences in calpain-3-deficient muscle

description: Additional file 2: FFold changes in WT and calpain-3 deficient myoblasts and myotubes.
DOI: 10.6084/m9.figshare.21856309
title: Development and optimization of metoclopramide containing polymeric patches: impact of permeation enhancers

description: Abstract The study is aimed to develop a monolithic controlled matrix transdermal patches containing Metoclopramide as a model drug by solvent casting method. Eudragit L100, Polyvinylpyrrolidone K-30, and Methylcellulose were used in different ratios and Polyethylene glycol 400 added as a plasticizer. Resulting patches were evaluated for their physicochemical characters like organoleptic characters, weight variation, folding endurance, thickness, swelling index, flatness, drug content, swelling index, percentage erosion, moisture content, water vapor transmission rate and moisture uptake. Formed patches were also evaluated through Fourier transform spectroscopy (FT-IR), X-ray diffraction (XRD), Differential Scanning calorimetry (DSC) and Scanning Electron Microscopy (SEM). Results of SEM unveiled smooth surface of drug-loaded patches. In-vitro dissolution studies were conducted by using dissolution medium phosphate buffer saline pH 7.4. Effect of natural permeation enhancers was elucidated on two optimized formulations (Z4 and Z9). Different concentrations (5%-10 %) of permeation enhancers i.e. Olive oil, Castor oil and Eucalyptus oil were evaluated on Franz diffusion cell using excised abdominal rat skin. Z4-O2 (Olive oil 10%) had enhanced sustain effect and flux value (310.72) close to the desired flux value. Z4-O2 followed Higuchi release model (R2= 0.9833) with non-fickian diffusion release mechanism (n=0.612).
DOI: 10.6084/m9.figshare.13466947
title: Additional file 4 of Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus

description: Additional file 4: Figure S2. Expression trends of the neuropeptide precursor genes in U. unicinctus larval transcriptome.
DOI: 10.6084/m9.figshare.14516297
title: Additional file 2 of Identification of a ferroptosis-related gene signature predictive model in colon cancer

description: Additional file 2. The detailed results of GO analysis.
DOI: 10.6084/m9.figshare.13466947.v1
title: Additional file 4 of Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus

description: Additional file 4: Figure S2. Expression trends of the neuropeptide precursor genes in U. unicinctus larval transcriptome.
DOI: 10.6084/m9.figshare.13467027
title: Additional file 8 of Loss of exosomal miR-146a-5p from cancer-associated fibroblasts after androgen deprivation therapy contributes to prostate cancer metastasis

description: Additional file 8: Table S1. Sequences of primers for PCR and reverse transcript.
DOI: 10.6084/m9.figshare.21856270.v1
title: Effects of polyvinylpyrrolidone and poly (ethylene glycol) on preparation of ibuprofen pharmaceutical cocrystal

description: Abstract In this study, we investigated the effects of polymers on the pharmaceutical cocrystal formation process. Ibuprofen (IBU) was selected as the active pharmaceutical ingredient (API), nicotinamide (NIC) and saccharin (SAC) as the cocrystal coformer (CCF), ethanol/water as the solvent, polyvinylpyrrolidone (PVP) and poly (ethylene glycol) (PEG) as the representative polymers. We prepared IBU-NIC and IBU-SAC cocrystals in ethanol-water cosolvent in the absence or presence of polymers. Cocrystal screening products were characterized by FTIR, DSC, PXRD, and HPLC. The results showed that the mixture of IBU and IBU-NIC cocrystal can be prepared in ethanol-water cosolvent without polymers. The addition of PVP facilitates the formation of pure IBU-NIC cocrystal; however, no cocrystal was formed in PEG solutions. SAC could not cocrystallize with IBU in the ethanol-water solvent in the absence of polymers. Neither PVP nor PEG could facilitate the formation of the IBU-SAC cocrystal.
DOI: 10.6084/m9.figshare.16614016
title: China's GHGs and APs data

description: This dataset contains an ensemble mean of China's GHGs and APs emissions for both sectorial and regional aspects.<br>
DOI: 10.6084/m9.figshare.9798305
title: Age-related DNA hydroxymethylation is enriched for gene expression and immune system processes in human peripheral blood

description: DNA methylation (DNAm) has a well-established association with age in many tissues, including peripheral blood mononuclear cells (PBMCs). Compared to DNAm, the closely related epigenetic modification known as DNA hydroxymethylation (DNAhm) was much more recently discovered in mammals. Preliminary investigations have observed a positive correlation between gene body DNAhm and cis-gene expression. While some of these studies have observed an association between age and global DNAhm, none have investigated region-specific age-related DNAhm in human blood samples. In this study, we investigated DNAhm and gene expression in PBMCs of 10 young and 10 old, healthy female volunteers. Thousands of regions were differentially hydroxymethylated in the old vs. young individuals in gene bodies, exonic regions, enhancers, and promoters. Consistent with previous work, we observed directional consistency between age-related differences in DNAhm and gene expression. Further, age-related DNAhm and genes with high levels of DNAhm were enriched for immune system processes which may support a role of age-related DNAhm in immunosenescence.
DOI: 10.6084/m9.figshare.9798305.v2
title: Age-related DNA hydroxymethylation is enriched for gene expression and immune system processes in human peripheral blood

description: DNA methylation (DNAm) has a well-established association with age in many tissues, including peripheral blood mononuclear cells (PBMCs). Compared to DNAm, the closely related epigenetic modification known as DNA hydroxymethylation (DNAhm) was much more recently discovered in mammals. Preliminary investigations have observed a positive correlation between gene body DNAhm and cis-gene expression. While some of these studies have observed an association between age and global DNAhm, none have investigated region-specific age-related DNAhm in human blood samples. In this study, we investigated DNAhm and gene expression in PBMCs of 10 young and 10 old, healthy female volunteers. Thousands of regions were differentially hydroxymethylated in the old vs. young individuals in gene bodies, exonic regions, enhancers, and promoters. Consistent with previous work, we observed directional consistency between age-related differences in DNAhm and gene expression. Further, age-related DNAhm and genes with high levels of DNAhm were enriched for immune system processes which may support a role of age-related DNAhm in immunosenescence.
DOI: 10.6084/m9.figshare.9798305.v1
title: Age-related DNA hydroxymethylation is enriched for gene expression and immune system processes in human peripheral blood.

description: DNA methylation (DNAm) has a well-established association with age in many tissues, including peripheral blood mononuclear cells (PBMCs). Compared to DNAm, the closely related epigenetic modification known as DNA hydroxymethylation (DNAhm) was much more recently discovered in mammals. Preliminary investigations have observed a positive correlation between gene body DNAhm and cis-gene expression. While some of these studies have observed an association between age and global DNAhm, none have investigated region-specific age-related DNAhm in human blood samples. In this study, we investigated DNAhm and gene expression in PBMCs of 10 young and 10 old, healthy female volunteers. Thousands of regions were differentially hydroxymethylated in the old vs. young individuals in gene bodies, exonic regions, enhancers, and promoters. Consistent with previous work, we observed directional consistency between age-related differences in DNAhm and gene expression. Further, age-related DNAhm and genes with high levels of DNAhm were enriched for immune system processes which may support a role of age-related DNAhm in immunosenescence.
DOI: 10.6084/m9.figshare.14516146.v1
title: Additional file 9 of Characteristics of epigenetic aging across gestational and perinatal tissues

description: Additional file 9. Table S3: Median (MED) coefficients, 95% confidence intervals (lcl = lower confidence limit; ucl = upper confidence limit) and % of non-zero of predictor variables across 1000 bootstrap samples for the final elastic net models.
DOI: 10.6084/m9.figshare.14516297.v1
title: Additional file 2 of Identification of a ferroptosis-related gene signature predictive model in colon cancer

description: Additional file 2. The detailed results of GO analysis.
DOI: 10.6084/m9.figshare.14516146
title: Additional file 9 of Characteristics of epigenetic aging across gestational and perinatal tissues

description: Additional file 9. Table S3: Median (MED) coefficients, 95% confidence intervals (lcl = lower confidence limit; ucl = upper confidence limit) and % of non-zero of predictor variables across 1000 bootstrap samples for the final elastic net models.
DOI: 10.6084/m9.figshare.14516125.v1
title: Additional file 2 of Characteristics of epigenetic aging across gestational and perinatal tissues

description: Additional file 2. Table S2: Mean estimated cell-type proportions (%) and SD in every data set.
DOI: 10.6084/m9.figshare.13467489.v1
title: Additional file 5 of Transcriptome analysis reveals modulation of the STAT family in PEDV-infected IPEC-J2 cells

description: Additional file 5: Supplementary Table 4. List of qRT-PCR primers.
DOI: 10.6084/m9.figshare.21856270
title: Effects of polyvinylpyrrolidone and poly (ethylene glycol) on preparation of ibuprofen pharmaceutical cocrystal

description: Abstract In this study, we investigated the effects of polymers on the pharmaceutical cocrystal formation process. Ibuprofen (IBU) was selected as the active pharmaceutical ingredient (API), nicotinamide (NIC) and saccharin (SAC) as the cocrystal coformer (CCF), ethanol/water as the solvent, polyvinylpyrrolidone (PVP) and poly (ethylene glycol) (PEG) as the representative polymers. We prepared IBU-NIC and IBU-SAC cocrystals in ethanol-water cosolvent in the absence or presence of polymers. Cocrystal screening products were characterized by FTIR, DSC, PXRD, and HPLC. The results showed that the mixture of IBU and IBU-NIC cocrystal can be prepared in ethanol-water cosolvent without polymers. The addition of PVP facilitates the formation of pure IBU-NIC cocrystal; however, no cocrystal was formed in PEG solutions. SAC could not cocrystallize with IBU in the ethanol-water solvent in the absence of polymers. Neither PVP nor PEG could facilitate the formation of the IBU-SAC cocrystal.
DOI: 10.6084/m9.figshare.14516300.v1
title: Additional file 3 of Identification of a ferroptosis-related gene signature predictive model in colon cancer

description: Additional file 3. The detailed results of GSEA.
DOI: 10.6084/m9.figshare.11370624
title: Mass spectrometry data from Protein aggregate formation permits millennium-old brain preservation

description: Supplementary Table: Proteins identified in the Helsington brain after subtration of proteins present in gel blank. Columns are: Accession = Swiss Prot accession number; "X10lgp" = protein identification score from the PEAKS Studio Software. Sequence.Coverage.Cx = percent of protein amino acid sequence covered by the identified peptides in cortex; Sequence.Coverage.WM = percent of protein amino acid sequence covered by the identified peptides in white matter; PTM = post-translational modifications identified in protein; Avg.Mass = average molecular mass of protein; Description = protein description.
DOI: 10.6084/m9.figshare.13466949.v1
title: Additional file 5 of Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus

description: Additional file 5: Figure S3. The negative controls of MIP, FRWa, FxFa and FILa in U. unicinctus larvae detected by whole-mount in situ hybridization.
DOI: 10.6084/m9.figshare.14516300
title: Additional file 3 of Identification of a ferroptosis-related gene signature predictive model in colon cancer

description: Additional file 3. The detailed results of GSEA.
DOI: 10.6084/m9.figshare.21856359
title: Efficacy and safety of fluticasone propionate nasal spray in treatment ofadenoidal hypertrophic snoring in children

description: Abstract This study investigatedthe efficacy and safety offluticasone propionate nasal spray in treatment of adenoidal hypertrophic snoring in children.Fifty-six children with adenoidal hypertrophic snoring were enrolled. According to adenoidal-nasopharyngeal ratio (ANR) in lateral nasal X-ray examination,the children were assigned in moderategroup (23 cases) and severegroup (33 cases).The fluticasone propionate nasal spray was used for all patientsfor 4 weeks.In 56 patients, after treatment, compared with before treatment, the snoring, sleep apneaand nasal obstruction scores in moderategroupand the nasal obstruction score in severegroupwere significantly decreased,respectively (P &lt; 0.05).The decreases of snoring, sleep apnea, mouth breathing and nasal obstruction scores after treatment in moderate group were significantly higher than those in severe group, respectively (P &lt;0.001).After treatment, in 18 patients with ateral nasal X-ray examination,the adenoid size was obviously reduced, and the nasopharynx airway was obviously enlarged. The meanANRdropped from 0.76±0.10 to 0.72±0.09(P &lt;0.001).Duringtreatment, only 2 of 56 patients were reported with intranasal dryness and occasional epistaxis, which were self-healed without treatment.Fluticasone propionate nasal spray is effective and safe for treatment of children’s snoring caused by adenoidal hypertrophy.