cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9976991.v1
title: SheepRedBloodCell_Immunized_spleen_746_1

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9977000.v1
title: AIDGFP_NonImmunized_spleen_746_3

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976985
title: NonImmunized_spleen_746_2

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976988
title: AIDGFP_SheepRedBloodCell_Immunized_spleen_725_1

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976973
title: NonImmunized_spleen_737_3

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976970
title: SheepRedBloodCell_Immunized_spleen725_2

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976973.v1
title: NonImmunized_spleen_737_3

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976988.v1
title: AIDGFP_SheepRedBloodCell_Immunized_spleen_725_1

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976967
title: NonImmunized_spleen_725_3

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976985.v1
title: NonImmunized_spleen_746_2

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976958
title: AIDGFP_SheepRedBloodCell_Immunized_spleen_737_1

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976961
title: SheepRedBloodCell_Immunized_spleen_737_2

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976958.v1
title: AIDGFP_SheepRedBloodCell_Immunized_spleen_737_1

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976970.v1
title: SheepRedBloodCell_Immunized_spleen725_2

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976961.v1
title: SheepRedBloodCell_Immunized_spleen_737_2

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976967.v1
title: NonImmunized_spleen_725_3

description: Negative ion mode IMS data were acquired with a 9.4T Bruker FT-ICR Solarix mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 2 kHz Nd: YAG (wavelength: 355nm) laser and an Infinity ion cyclotron resonance (ICR) cell. Laser power was optimized for each sample by adjusting in the range of 18-20%. Data were collected in negative ion mode from <i>m/z</i> 200-2,000 with a raster step of 30 µm and a data size of 1 MB per spectrum with a free induction decay (FID) length of 0.8389 s. Each pixel consisted of 500 laser shots with the minimum laser energy focus setting (~10 µm) at a frequency of 2000 Hz, and a smart walk of 25 µm was enabled to increase sensitivity. The mass-selecting quadrupole was set to <i>m/z</i> 350 and TOF of 0.8 ms. The resolving power of all imaging experiments was ~80,000 at <i>m/z</i> 766.54. External calibration was performed prior to analysis and internal lock-mass calibration was performed using <i>m/z</i> 885.5494 ([PI(18:0_20:4)-H]<sup>-</sup>) during image acquisition.
DOI: 10.6084/m9.figshare.9976883
title: Disparate responses to salinity across species and organizational levels in anchialine shrimps

description: Abstract:<br>Environmentally-induced plasticity in gene expression is one of the underlying mechanisms of adaptation to habitats with variable environments. For example, euryhaline crustaceans show predictable changes in the expression of ion-transporter genes during salinity transfers, although studies have typically been limited to specific genes, taxa, and ecosystems of interest. Here, we investigated responses to salinity change at multiple organizational levels in five species of shrimps representing at least three independent invasions of the anchialine ecosystem, defined as habitats with marine and freshwater influences with spatial and temporal fluctuations in salinity. Although all five species were generally strong osmoregulators, salinity-induced changes in gill physiology and gene expression were highly species-specific. While some species exhibited patterns similar to previously studied euryhaline crustaceans, instances of distinct and atypical patterns were recovered from closely related species. Species-specific patterns were found when examining: 1) numbers and identities of differentially expressed genes, 2) salinity-induced expression of genes predicted a priori to play a role in osmoregulation, and 3) salinity-induced expression of orthologs shared among all species. Notably, ion transport genes were unchanged in the atyid Halocaridina rubra while ones normally associated with vision and light perception were among those most highly upregulated. Potential reasons for species-specific patterns are discussed, including variation among anchialine habitats in salinity regimes and divergent evolution in anchialine taxa. Underexplored mechanisms of osmoregulation in crustaceans revealed here by the application of transcriptomic approaches to ecologically and taxonomically understudied systems are also explored.<br>Supplementary datasets and information:<br>Here:<br>you will find all the supplementary information referred to in the paper (tables, methods, figures are in the file SI_101319.pdf) as well as two different datasets on gene expression, which are described in the pdf and in the README sheets of the datasets.
DOI: 10.6084/m9.figshare.9976883.v1
title: Disparate responses to salinity across species and organizational levels in anchialine shrimps

description: Abstract:<br>Environmentally-induced plasticity in gene expression is one of the underlying mechanisms of adaptation to habitats with variable environments. For example, euryhaline crustaceans show predictable changes in the expression of ion-transporter genes during salinity transfers, although studies have typically been limited to specific genes, taxa, and ecosystems of interest. Here, we investigated responses to salinity change at multiple organizational levels in five species of shrimps representing at least three independent invasions of the anchialine ecosystem, defined as habitats with marine and freshwater influences with spatial and temporal fluctuations in salinity. Although all five species were generally strong osmoregulators, salinity-induced changes in gill physiology and gene expression were highly species-specific. While some species exhibited patterns similar to previously studied euryhaline crustaceans, instances of distinct and atypical patterns were recovered from closely related species. Species-specific patterns were found when examining: 1) numbers and identities of differentially expressed genes, 2) salinity-induced expression of genes predicted a priori to play a role in osmoregulation, and 3) salinity-induced expression of orthologs shared among all species. Notably, ion transport genes were unchanged in the atyid Halocaridina rubra while ones normally associated with vision and light perception were among those most highly upregulated. Potential reasons for species-specific patterns are discussed, including variation among anchialine habitats in salinity regimes and divergent evolution in anchialine taxa. Underexplored mechanisms of osmoregulation in crustaceans revealed here by the application of transcriptomic approaches to ecologically and taxonomically understudied systems are also explored.<br>Supplementary datasets and information:<br>Here:<br>you will find all the supplementary information referred to in the paper (tables, methods, figures are in the file SI_101319.pdf) as well as two different datasets on gene expression, which are described in the pdf and in the README sheets of the datasets.
DOI: 10.6084/m9.figshare.9976790
title: SM Table 4. Summary of SHRIMP U–Pb zircon results for sample 14020. Mid-crustal deformation in a continental margin orogen: structural evolution and timing of the Famatinian Orogeny, NW Argentina

description: SM Table 4. Summary of SHRIMP U–Pb zircon results for sample 14020.
DOI: 10.6084/m9.figshare.9976787
title: SM Table 5. Summary of Ar–Ar mica results. Mid-crustal deformation in a continental margin orogen: structural evolution and timing of the Famatinian Orogeny, NW Argentina

description: SM Table 5. Summary of Ar–Ar mica results
DOI: 10.6084/m9.figshare.9976784
title: SM Table 3. U–Pb LA–MC-ICP–MS geochronology. Mid-crustal deformation in a continental margin orogen: structural evolution and timing of the Famatinian Orogeny, NW Argentina

description: SM Table 3. U–Pb LA–MC-ICP–MS geochronology.
DOI: 10.6084/m9.figshare.9976790.v1
title: SM Table 4. Summary of SHRIMP U–Pb zircon results for sample 14020. Mid-crustal deformation in a continental margin orogen: structural evolution and timing of the Famatinian Orogeny, NW Argentina

description: SM Table 4. Summary of SHRIMP U–Pb zircon results for sample 14020.
DOI: 10.6084/m9.figshare.9976787.v1
title: SM Table 5. Summary of Ar–Ar mica results. Mid-crustal deformation in a continental margin orogen: structural evolution and timing of the Famatinian Orogeny, NW Argentina

description: SM Table 5. Summary of Ar–Ar mica results
DOI: 10.6084/m9.figshare.9976784.v1
title: SM Table 3. U–Pb LA–MC-ICP–MS geochronology. Mid-crustal deformation in a continental margin orogen: structural evolution and timing of the Famatinian Orogeny, NW Argentina

description: SM Table 3. U–Pb LA–MC-ICP–MS geochronology.