DOI: 10.5061/dryad.qnk98sfpp
title: Data from: Homeostatic control of deep sleep and molecular correlates of sleep pressure in Drosophila

description: Homeostatic control of sleep is typically addressed through mechanical
 stimulation-induced forced wakefulness and the measurement of subsequent
 increases in sleep. A major confound attends this approach: biological
 responses to deprivation may reflect a direct response to the mechanical
 insult rather than to the loss of sleep. Similar confounds accompany all
 forms of sleep deprivation and represent a major challenge to the field.
 Here we describe a new paradigm for sleep deprivation in Drosophila that
 fully accounts for sleep-independent effects. Our results reveal that deep
 sleep states are the primary target of homeostatic control and establish
 the presence of multi-cycle sleep rebound following deprivation.
 Furthermore, we establish that specific deprivation of deep sleep state
 results in state-specific homeostatic rebound. Finally, by accounting for
 the molecular effects of mechanical stimulation during deprivation
 experiments, we show that serotonin levels track sleep pressure in the
 fly’s central brain. Our results illustrate the critical need to control
 for sleep-independent effects of deprivation when examining the molecular
 correlates of sleep pressure and call for a critical reassessment of work
 that has not accounted for such non-specific effects.
description: All behavioral data were collected using either the Drosophila
 Activity Monitors or the Ethoscope platform (as described in the methods
 section of our manuscript). MALDI-TOF MS data were collected using the
 Bruker Autoflex Speed TOF Machine. Processed data are uploaded as a
 compressed file.
description: # Homeostatic control of deep sleep and molecular correlates of sleep
 pressure in Drosophila The zipped file contains 4 folders. The folders
 are: Figure1, Figures 2 to 4, Figure5 and Figure6. # Description of the
 data and file structure The folder 'Figure1' has three
 subfolders, i.e., '6h deprivation', '12h deprivation'
 and '24h deprivation'. Within each of these folders, there are
 .txt files with the raw sleep data for all individual replicates of the
 deprived and undisturbed control flies reported in Figure 1 of the
 manuscript. For instance, Figure1 &gt; 6h deprivation &gt;
 Control_20sec_SleepData.txt contains the sleep data for undisturbed flies
 for the 20 second trigger experiment. Similar files for the deprived flies
 and for different trigger frequencies are uplaoded within each of the
 three subfolders. The first column in all of these text files is the time
 information in Zeitgeber Time (ZT) units. Each subsequent column has the
 individual fly's sleep data. The folder 'Figures 2 to 4'
 has .txt files for Canton-S (CS) and w1118 flies for all treatments
 (focal, yoked, and undisturbed). These were collected through the
 Ethoscope platform and the deprivation trigger for all these experiments
 was 220-seconds. For w1118 flies, data for three cycles and 5-min
 definition of sleep are provided. For Canton-S (CS), data for five cycles
 and both 5-min and 25-min definition of sleep are provided. These data
 were used in Figures 2 to 4 of the corresponding manuscript. The first
 column in each of these text files is the time information in Zeitgeber
 Time (ZT) units. Each subsequent column has the individual fly's
 sleep data. The folder 'Figure5' has .txt files for Canton-S
 (CS) flies for all treatments (focal, yoked, and undisturbed). These were
 collected through the Ethoscope platform and the deprivation trigger for
 all these experiments was 22-minutes. For Canton-S, data for five cycles
 and both 5-min and 25-min definition of sleep are provided. These data
 were used in Figure 5 of the corresponding manuscript. The first column in
 each of these text files is the time information in Zeitgeber Time (ZT)
 units. Each subsequent column has the individual fly's sleep data.
 The folder 'Figure6' has two .txt files, one each from the two
 independent MALDI-TOF experiments. Each text file has three columns. The
 first column reports the m/z (mz) value, the second column reports the
 treatment condition, and the third column reports the intensity of each of
 these detected molecules. # Code/Software All these files are executable
 in MS Excel, any spreadsheet program, or a text editor.