DOI: 10.5061/dryad.v56g3
title: Data from: Characterization and distribution of mating-type genes of the turfgrass pathogen Sclerotinia homoeocarpa on a global scale

description: Sclerotinia homoeocarpa F.T. Bennett is a filamentous member of Ascomycota
 that causes dollar spot, the most economically important disease of
 turfgrass worldwide. We sequenced and characterized the mating-type (MAT)
 locus of four recently-collected contemporary strains causing dollar spot,
 four historical type strains used to describe the fungus, and three
 species of Rutstroemiaceae. Moreover, we developed a multiplex PCR assay
 to screen 1019 contemporary isolates for mating-type. The organization of
 the MAT loci of all strains examined could be classified into one of four
 categories: (1) putatively heterothallic, as exemplified by all
 contemporary strains and three of four historical type strains; (2)
 putatively heterothallic with a deleted putative gene in the MAT1-2
 idiomorph, as detected in strains from two recently-collected populations
 in the United Kingdom that show more similarity to historical strains; (3)
 putatively homothallic with close physical linkage between MAT1-1-1 and
 MAT1-2-1, as found in one historical type strain of S. homoeocarpa and two
 strains of Rutstroemia cuniculi; and (4) an unresolved but apparently
 homothallic organization in which strains contained both MAT1-1-1 and
 MAT1-2-1 but linkage between these genes and between the two flanking
 genes could not be confirmed, as identified in R. paludosa and Poculum
 henningsianum. In contemporary S. homoeocarpa populations there was no
 significant difference in the frequency of the two mating types in
 clone-corrected samples when analyzed on regional and local scales,
 suggesting sex may be possible in this pathogen. However, two isolates
 from Italy and twenty from California were heterokaryotic for both
 complete heterothallic MAT idiomorphs. Results from this study contribute
 to knowledge about mating systems in filamentous fungi and enhance our
 understanding of the evolution and biology of an important plant pathogen.
description: Size and features of
 Ascomycota mating type lociCursory literature
 review of the size and features of Ascomycota mating type
 lociPutman_MAT-Sh_FGB_MAT-locus-lit-search.xlsxAnnotation of the Sclerotinia homoeocarpa mating type locusRepeatModeler, RepeatMasker, and GeneMark commands; repeat library; and gene models output from GeneMark-ES v2.3c betaFGB-14-335R2_Sh-MAT-locus_annotation.zipAnalysis of worldwide distribution of Sclerotinia homoeocarpa mating type genesAnalysis of clone-corrected ratios of mating type using binomial tests with simple continuity correctionPutman_MAT-Sh_FGB_Tbl-4-5_MAT-distribution-analysis.xlsxAnalysis of duplicated MAT1-2-1 of Sclerotinia homoeocarpaAmino acid sequences, alignments, and MetaPIGA run parameters used to compare the truncated copy of MAT1-2-1 found outside the mating type locus of the MAT1-1 Sclerotinia homoeocarpa strain MB-01FGB-14-335R2_Sh-MAT-locus_duplicated.zipPhylogeny of the mating type and flanking genes.Sequences, alignments, and MetaPIGA run files used to infer the phylogenies of the mating type (MAT1-2-1, MAT1-2-4, MAT1-1-5, and MAT1-1-1) and flanking (APN2 and SLA2) genes.FGB-14-335R2_Sh-MAT-locus_phylogeny.zipRepeat features of the Sclerotinia homoeocarpa mating type locusShort sequence segments used to analyze the inverted and direct repeat features bordering the mating type idiomorphs.FGB-14-335R2_Sh-MAT-locus_repeat_features.zipSanger sequencing readsSanger reads used to sequence the mating type loci.sequencing_reads.zip