DOI: 10.5061/dryad.5h1q6mc
title: Data from: Repeated evolution of asymmetric genitalia and right-sided mating behavior in the Drosophila nannoptera species group

description: Background: Male genitals have repeatedly evolved left-right asymmetries,
 and the causes of such evolution remain unclear. The Drosophila nannoptera
 group contains four species, among which three exhibit left-right
 asymmetries of distinct genital organs. In the most studied species,
 Drosophila pachea, males display asymmetric genital lobes and they mate
 right-sided on top of the female. Copulation position of the other species
 is unknown. Results: To assess whether the evolution of genital asymmetry
 could be linked to the evolution of one-sided mating, we examined phallus
 morphology and copulation position in D. pachea and closely related
 species. The phallus was found to be symmetric in all investigated species
 except D. pachea, which display an asymmetric phallus with a right-sided
 gonopore, and D. acanthoptera, which harbor an asymmetrically bent
 phallus. In all examined species, males were found to position themselves
 symmetrically on top of the female, except in D. pachea and D. nannoptera,
 where males mated right-sided, in distinctive, species-specific positions.
 In addition, the copulation duration was found to be increased in
 nannoptera group species compared to closely related outgroup species.
 Conclusion: Our study shows that gains, and possibly losses, of asymmetry
 in genital morphology and mating position have evolved repeatedly in the
 nannoptera group. Current data does not allow us to conclude whether
 genital asymmetry has evolved in response to changes in mating position,
 or vice versa.
description: Data - Figure
 1Series of Scanning
 Electron Microscopy (SEM) images, phallus D. pachea, two
 individualsdata-Figure-1.tar.gzData - Additional File 1-2Light Microscopy images, phallus preparations of Drosophila acanthoptera, Drosophila pachea, Drosophila nannoptera, Drosophila machalilla and Drosophila bromeliaedata-additional-file-1-2.tar.gzData - Additional File 3Sequence data: data-additional-file-3.nex for the molecular phylogeny presented in supplementary Figure 6; BEAST input file: phylo-180912.xmldata-additional-file-3.tar.gzData - Additional File 4, Part 1 (Daca_Dbro_Dbuz)Movies, multi-species mating posture analysis, data used to prepare additional datafile 4. Files were converted to .webm format in order to reduce file size.data-additional-file-4_part1_Daca_Dbro_Dbuz.tar.gzData - Additional File 4, Part 2 (Dmac)Movies, multi-species mating posture analysis, data used to prepare additional datafile 4. Files were converted to .webm format in order to reduce file size.data-additional-file-4_part2_Dmac.tar.gzData - Additional File 4, Part 3 (Dmel_Dmoj_Dnan_Dtri_Dwil)Movies, multi-species mating posture analysis, data used to prepare additional datafile 4. Files were converted to .webm format in order to reduce file size.data-additional-file-4_part3_Dmel_Dmoj_Dnan_Dtri_Dwil.tar.gzData - Additional File 7-8Movies, Drosophila nannoptera, frontal view of copulation, data used to prepare additional datafiles 7 and 8. Files were converted to .webm format in order to reduce file size.data-additional-file-7-8.tar.gz