cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/z6m19-5mw47
title: Rhesus TRIM5α decorated HIV-1

description: Non infectious hyperstable HIV-1 cores decorated with Rhesus TRIM5α. 0.3 µM sulfo-EGS. Cores were heavily coated.
description: <strong>Tilt Series Date:</strong> 2015-06-14</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -5.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Wes Sunquist, Barbie Ganser-Pornillos</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Yen-Li (Wes Sundquist Lab)</p>
<strong>Sample Preparation:</strong> Quantifoil 300 mesh R2/2</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-14/rawdata/</p>tomographycores0014.mrc,  Tilt Series (Pixel Size 2.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-14/rawdata/tomographycores0014.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomographycores0014_full.rec,  Reconstruction (Pixel Size 0.0 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-14/3dimage_76029/tomographycores0014_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cbvb0-ztj42
title: T5_wt_control_Aumalemaide_5nm

description: This is a wt sample in which Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. This is a control sample. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-02-14</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-5/rawdata/</p>sq90005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-5/rawdata/sq90005.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq90005.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-5/3dimage_82400/sq90005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r08xp-mv040
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-34/3dimage_71465/</p>atlas1_sq40037.rec,  Reconstruction (Pixel Size 1.28 nm), 711.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-34/3dimage_71465/atlas1_sq40037.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40037.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-34/rawdata/atlas1_sq40037.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q1hbb-kf036
title: INS-1E with Calreticulin-YFP

description: CLEM and ECT
description: <strong>Tilt Series Date:</strong> 2016-04-17</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 0.47368421 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zachary Freyberg from University of Pittsburgh provided the frozen cells on grids. Collaboration via Stephen Carter. CLEM and ECT done by me.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-10/rawdata/</p>Calreticulin-TS25.st,  Tilt Series (Pixel Size 0.6 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-10/rawdata/Calreticulin-TS25.st"
        > <i class="download icon"></i> Download </a></p>

        Calreticulin-TS25_full.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-10/3dimage_74338/Calreticulin-TS25_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b2dwt-ze210
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-312/rawdata/</p>T4SSWTNov26A019_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-312/rawdata/T4SSWTNov26A019_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov26A019_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-312/3dimage_105065/T4SSWTNov26A019_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.dd3hvx
title: Occurrence Download

description: A dataset containing 1185 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "TaxonKey is Gentiana rhodantha"
 ]
}

 The dataset includes 1185 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045146-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wfssua
title: Occurrence Download

description: A dataset containing 337 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "Geometry POLYGON((93 29,112 29,112 43,93 43,93 29))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Artemisia sieversiana"
 ]
}

 The dataset includes 337 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045145-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/gmqpg-4dp06
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-484/rawdata/</p>T4SSWTminiNov21A019_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-484/rawdata/T4SSWTminiNov21A019_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTminiNov21A019_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-484/3dimage_105206/T4SSWTminiNov21A019_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2kkvq-3w247
title: T5_wt_control_Aumalemaide_5nm

description: This is a wt sample in which Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. This is a control sample. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-02-14</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-20/rawdata/</p>sq90020.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-20/rawdata/sq90020.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq90020.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-14-20/3dimage_82429/sq90020.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bxz5m-jz925
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-257/rawdata/</p>T4SSWTDec12B007_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-257/rawdata/T4SSWTDec12B007_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTDec12B007_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-257/3dimage_105013/T4SSWTDec12B007_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ykmtg9
title: Occurrence Download

description: A dataset containing 20 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Actinodura nipalensis",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 20 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045147-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/53k4p-eye80
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-5/3dimage_71406/</p>atlas1_sq40008_full.rec,  Reconstruction (Pixel Size 1.28 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-5/3dimage_71406/atlas1_sq40008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40008.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-5/rawdata/atlas1_sq40008.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.qjkqaz
title: Occurrence Download

description: A dataset containing 264910 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Phoca vitulina",
 "Year 2000-2025"
 ]
}

 The dataset includes 264910 records from 282 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045060-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/jrpmk-7ft51
title: T9_wt

description: This was a wt sample prepared with Paul when he was visiting
description: <strong>Tilt Series Date:</strong> 2016-04-30</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Paul Veith and Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> See pages 84-86</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-04-30-88/rawdata/</p>atlas10080.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-04-30-88/rawdata/atlas10080.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10080_part121_13.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-04-30-88/3dimage_74680/atlas10080_part121_13.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/175kt-jqf17
title: PW3654

description: Pseudomonas aeruginosa, strain PW3654.Acquired from the Transposon mutant collection. PW3654- lacZwp06q3 (CheW of F6 system)
description: <strong>Tilt Series Date:</strong> 2015-02-23</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
  aba2015-02-23-49_slicer12788.jpg: short array (magenta) .  long array (green) .  pilus (blue)
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-49/rawdata/</p>PseudomonasdeltaFF6CheWNEWFEB0007.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-49/rawdata/PseudomonasdeltaFF6CheWNEWFEB0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        PseudomonasdeltaFF6CheWNEWFEB0007_part121_10.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-49/3dimage_52279/PseudomonasdeltaFF6CheWNEWFEB0007_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7t92k-w3n23
title: nanochemowt_2ndday

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-37/rawdata/</p>nanochemowt2day_manual1.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-37/rawdata/nanochemowt2day_manual1.mrc"
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        nanochemowt2day_manual1_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
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