cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7743470
title: Water-Soluble Benzazole Dyes Fluorescent by ESIPT: Structural Characterization, Photophysical Properties and Its Application as a Probe for Direct Staining of Helminths

description: A series of four water-soluble benzazole dyes that emit fluorescence by the excited state intramolecular proton transfer (ESIPT) mechanism were structurally characterized by Fourier transform infrared spectroscopy (FTIR), 1H and 13C nuclear magnetic resonance (NMR) attached proton test (APT) and mass spectrometry. Their photophysical properties were systematically studied by UV-absorption and fluorescence emission. Some photophysical parameters were obtained by semi-empirical PM3 and ZINDO methods, and related to experimental photophysical data. Changes in the absorption and fluorescence emission spectra as well as conformational equilibrium between different species were investigated in solvents of different polarities and under different pH conditions. These benzazole dyes emit dual fluorescence emission in the blue-orange region with a Stokes shift between 2617-12337 cm-1. In general, these dyes are potentially interesting for studies of biological systems in an aqueous environment due to the presence of groups that increase aqueous solubility and reactivity with biomolecules. The present dyes were successfully used as new probes by means of direct staining of larvae. The obtained results indicate that these fluorescent dyes permit a quick, easy and selective visualization of larvae.
DOI: 10.6084/m9.figshare.7743476
title: Serotonin induces or inhibits neuritic regeneration of leech CNS neurons depending on neuronal identity

description: Recovery of motor function after central nervous system (CNS) injury is dependent on the regeneration capacity of the nervous system, which is a multifactorial process influenced, among other things, by the role of neuromodulators such as serotonin. The neurotransmitter serotonin can promote neuronal regeneration but there are also reports of it causing restriction, so it is important to clarify these divergent findings in order to understand the direct scope and side effects of potential pharmacological treatments. We evaluated the effect of serotonin on the extent of neuritic outgrowth and morphology of three different neuronal types in the leech Haementeria officinalis during their regeneration in vitro: Retzius interneurons (Rz), annulus erector (AE) motoneurons, and anterolateral number 1 (AL1) CNS neurons. Neurons were isolated and cultured in L15 medium, with or without serotonin. Growth parameters were registered and quantified, and observed differences were analyzed. The addition of serotonin was found to induce AL1 neurons to increase their average growth dramatically by 8.3-fold (P=0.02; n=5), and to have no clear effect on AE motoneurons (P=0.44; n=5). For Rz interneurons, which normally do not regenerate their neurites, the addition of concanavaline-A causes substantial growth, which serotonin was found to inhibit on average by 98% (P=0.02; n=5). The number of primary neurites and their branches were also affected. These results reveal that depending on the neuronal type, serotonin can promote, inhibit, or have no effect on neuronal regeneration. This suggests that after CNS injury, non-specific pharmacological treatments affecting serotonin may have different effects on different neuronal populations.
DOI: 10.7284/107538
title: GNSS (Global Navigation Satellite System) data collected during research cruise AMAT02RR using a Ashtech ADU2 instrument system onboard the platform RV Roger Revelle

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743464
title: The Effects of Trimetazidine on QT-interval Prolongation and Cardiac Hypertrophy in Diabetic Rats

description: Abstract Background: Trimetazidine (TMZ) is an anti-ischemic drug. In spite of its protective effects on cardiovascular system, there is no scientific study on the usefulness of TMZ treatment for prolonged QT interval and cardiac hypertrophy induced by diabetes. Objectives: To evaluate the effects of TMZ on QT interval prolongation and cardiac hypertrophy in the diabetic rats. Methods: Twenty-four male Sprague-Dawley rats (200-250 g) were randomly assigned into three groups (n = 8) by simple random sampling method. Control (C), diabetic (D), and diabetic administrated with TMZ at 10 mg/kg (T10). TMZ was administrated for 8 weeks. The echocardiogram was recorded before isolating the hearts and transfer to a Langendorff apparatus. Hemodynamic parameters, QT and corrected QT interval (QTc) intervals, heart rate and antioxidant enzymes were measured. The hypertrophy index was calculated. The results were evaluated by one-way ANOVA and paired t-test using SPSS (version 16) and p &lt; 0.05 was regarded as significant. Results: The diabetic rats significantly indicated increased hypertrophy, QT and QTc intervals and decreased Left ventricular systolic pressure (LVSP), Left ventricular developed pressure (LVDP), rate pressure product (RPP), Max dp/dt, and min dp/dt (±dp/dt max), heart rate, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase in the heart. Treatment with TMZ in the diabetic animals was significantly improved these parameters in comparison to the untreated diabetic group. Conclusions: TMZ improves QTc interval prolongation and cardiac hypertrophy in diabetes.
DOI: 10.6084/m9.figshare.7743467.v1
title: Effects of regulatory changes in microcredit on the financial and social performance of Brazilian credit unions

description: ABSTRACT This study investigates whether the performance of credit unions that offer microcredit in Brazil was affected by the advent of Crescer - the National Microcredit Program. This research fills a gap in the literature because few papers investigate credit unions that work with microcredit and also the effects of governmental interventions related to microcredit operations. Studies of this type may help evaluate the impact of governmental interventions on the performance of the institutions that are directly or indirectly affected. Our results add to the debate about microcredit and about the inclusion of credit unions in this market. In particular, our evidence may influence the design of public policies and the strategies of microfinance institutions, which typically combine economic and social objectives. Based on the literature, we calculate fifteen indicators for each credit union, related to their financial and social performance. The inferences are based on the implementation of the difference in differences estimator using the advent of Crescer, in 2011, as the exogenous event of interest and including in the control group the credit unions that did not provide microcredit loans throughout the sample period. This research presents evidence that the volume of clients and microcredit operations performed by Brazilian credit unions was positively affected by regulatory changes that took place in 2011, consistently with the objectives of the governmental intervention. The evidence also suggests that the governmental intervention did not harm the financial sustainability of the credit unions. The main changes are: a substantial reduction of interest rates and transaction costs, the implementation of subsidies to participants in the program, and a push for public banks to enhance their supply of productive and oriented microcredit.
DOI: 10.6084/m9.figshare.7743476.v1
title: Serotonin induces or inhibits neuritic regeneration of leech CNS neurons depending on neuronal identity

description: Recovery of motor function after central nervous system (CNS) injury is dependent on the regeneration capacity of the nervous system, which is a multifactorial process influenced, among other things, by the role of neuromodulators such as serotonin. The neurotransmitter serotonin can promote neuronal regeneration but there are also reports of it causing restriction, so it is important to clarify these divergent findings in order to understand the direct scope and side effects of potential pharmacological treatments. We evaluated the effect of serotonin on the extent of neuritic outgrowth and morphology of three different neuronal types in the leech Haementeria officinalis during their regeneration in vitro: Retzius interneurons (Rz), annulus erector (AE) motoneurons, and anterolateral number 1 (AL1) CNS neurons. Neurons were isolated and cultured in L15 medium, with or without serotonin. Growth parameters were registered and quantified, and observed differences were analyzed. The addition of serotonin was found to induce AL1 neurons to increase their average growth dramatically by 8.3-fold (P=0.02; n=5), and to have no clear effect on AE motoneurons (P=0.44; n=5). For Rz interneurons, which normally do not regenerate their neurites, the addition of concanavaline-A causes substantial growth, which serotonin was found to inhibit on average by 98% (P=0.02; n=5). The number of primary neurites and their branches were also affected. These results reveal that depending on the neuronal type, serotonin can promote, inhibit, or have no effect on neuronal regeneration. This suggests that after CNS injury, non-specific pharmacological treatments affecting serotonin may have different effects on different neuronal populations.
DOI: 10.6084/m9.figshare.7743458
title: Factors associated with the structural liquidity of banks in Brazil

description: ABSTRACT This study aimed to identify the relationship between the Structural Liquidity Index (SLI) and macroeconomic variables, bank characteristics, and the validity period of the Basel III Agreement. Although the academic discussion on bank liquidity essentially addresses short-term issues, monitoring long-term liquidity helps assess any excessive dependency of banks on unstable resources, thus contributing to mitigating the risks of systemic liquidity crises such as that of 2008. As it provides evidence of the relationship between the SLI and the selected explanatory variables, the study can be included in the context of the discussions involving the Basel III Agreement, which establishes the implementation of the long-term liquidity index regulatory requirement as of 2018. The model was formulated based on fourteen research hypotheses, tested using panel data regressions estimated via pooled ordinary least squares, least squares with fixed effects, and two-stage least squares with fixed effects. The dependent variable was constructed based on the accounting data of 184 conglomerates and individual financial institutions operating in the country between June 2002 and December 2014. The SLI presented a positive relationship with changes in the exchange rate, international reserves, and reserve requirements, as well as with the profitability, size, and main specialization of the institution. On the other hand, we found a negative relationship between the SLI and the basic interest rate, country risk, balance of trade, validity period of the Basel III Agreement, type of equity control (private vs. government), and the bank being publicly listed on the São Paulo Stock, Commodities, and Futures Exchange (BM&FBovespa) or privately held. The validation of the relationships between these explanatory variables and the SLI provides a broader understanding of the risks to which financial institutions are exposed, contributing to the preventive analysis of bank liquidity risk - an antecedent indicator of financial crises, diminished confidence, and economic instability.
DOI: 10.6084/m9.figshare.7743464.v1
title: The Effects of Trimetazidine on QT-interval Prolongation and Cardiac Hypertrophy in Diabetic Rats

description: Abstract Background: Trimetazidine (TMZ) is an anti-ischemic drug. In spite of its protective effects on cardiovascular system, there is no scientific study on the usefulness of TMZ treatment for prolonged QT interval and cardiac hypertrophy induced by diabetes. Objectives: To evaluate the effects of TMZ on QT interval prolongation and cardiac hypertrophy in the diabetic rats. Methods: Twenty-four male Sprague-Dawley rats (200-250 g) were randomly assigned into three groups (n = 8) by simple random sampling method. Control (C), diabetic (D), and diabetic administrated with TMZ at 10 mg/kg (T10). TMZ was administrated for 8 weeks. The echocardiogram was recorded before isolating the hearts and transfer to a Langendorff apparatus. Hemodynamic parameters, QT and corrected QT interval (QTc) intervals, heart rate and antioxidant enzymes were measured. The hypertrophy index was calculated. The results were evaluated by one-way ANOVA and paired t-test using SPSS (version 16) and p &lt; 0.05 was regarded as significant. Results: The diabetic rats significantly indicated increased hypertrophy, QT and QTc intervals and decreased Left ventricular systolic pressure (LVSP), Left ventricular developed pressure (LVDP), rate pressure product (RPP), Max dp/dt, and min dp/dt (±dp/dt max), heart rate, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase in the heart. Treatment with TMZ in the diabetic animals was significantly improved these parameters in comparison to the untreated diabetic group. Conclusions: TMZ improves QTc interval prolongation and cardiac hypertrophy in diabetes.
DOI: 10.6084/m9.figshare.7743452
title: Limnology of shallow lakes of the semi-arid Central Pampa of Argentina

description: Abstract Aim The Central Pampa of Argentina has three recognized phytogeographic regions that arise due to the decrease in rainfall towards the west. The area has numerous lakes that are mainly temporary, with hydroperiods that relate to climatic cycles, although some of them have changed due to anthropogenic influence. Some of these lakes have been studied with special reference to zooplankton, but information on their physical and chemical aspects is scarce. Consequently, managing and evaluating the anthropogenic effects on these ecosystems is challenging. The objective of this study was to explore the limnological characteristics of lakes in different regions in the Central Pampa that experience different anthropic influences. Methods Ten lakes were sampled seasonally (January, April, July, and October) during 2007. In situ measurements included transparency, pH, temperature, and dissolved oxygen concentration, and 2-L water samples were collected to determine salinity, ion composition, suspended solids, nutrient concentration, and phytoplankton chlorophyll- a concentration. Results Salinity ranged from 0.32-136.72 g L-1, with Na+ being the dominant ion in nine lakes and Cl- and HCO3- predominating in the higher- and lower-salinity lakes respectively. Nutrient concentrations were high (total Kjeldahl nitrogen: 7.97-34.69 mg L-1; total phosphorous: 4.07-14.82 mg L-1), and all lakes were hypertrophic. We determined three lake classes: i) lakes transformed from low-salinity lakes into hypersaline ones through human inactivation of the fluvial system that fed it; ii) mesosaline temporary lakes lacking fish, with low concentrations of chlorophyll-a and influenced by agricultural activities, and iii) subsaline and hyposaline lakes, highly modified by urban sewage, converted in permanent lakes (which allowed fish fauna development) and with reduced water transparency (due to high concentrations of phytoplankton chlorophyll-a). Conclusions The chemical diversity of the studied lakes is low, and their predominance of Na+ and Cl- indicated that evaporation and crystallization control the water chemistry. Additionally, this study showed the consequences of the anthropic impact, which alter water chemical composition, trophic structure and, thus, the ecological characteristics of lakes.
DOI: 10.6084/m9.figshare.7743458.v1
title: Factors associated with the structural liquidity of banks in Brazil

description: ABSTRACT This study aimed to identify the relationship between the Structural Liquidity Index (SLI) and macroeconomic variables, bank characteristics, and the validity period of the Basel III Agreement. Although the academic discussion on bank liquidity essentially addresses short-term issues, monitoring long-term liquidity helps assess any excessive dependency of banks on unstable resources, thus contributing to mitigating the risks of systemic liquidity crises such as that of 2008. As it provides evidence of the relationship between the SLI and the selected explanatory variables, the study can be included in the context of the discussions involving the Basel III Agreement, which establishes the implementation of the long-term liquidity index regulatory requirement as of 2018. The model was formulated based on fourteen research hypotheses, tested using panel data regressions estimated via pooled ordinary least squares, least squares with fixed effects, and two-stage least squares with fixed effects. The dependent variable was constructed based on the accounting data of 184 conglomerates and individual financial institutions operating in the country between June 2002 and December 2014. The SLI presented a positive relationship with changes in the exchange rate, international reserves, and reserve requirements, as well as with the profitability, size, and main specialization of the institution. On the other hand, we found a negative relationship between the SLI and the basic interest rate, country risk, balance of trade, validity period of the Basel III Agreement, type of equity control (private vs. government), and the bank being publicly listed on the São Paulo Stock, Commodities, and Futures Exchange (BM&FBovespa) or privately held. The validation of the relationships between these explanatory variables and the SLI provides a broader understanding of the risks to which financial institutions are exposed, contributing to the preventive analysis of bank liquidity risk - an antecedent indicator of financial crises, diminished confidence, and economic instability.
DOI: 10.7284/107544
title: Gravimeter data collected during research cruise AMAT02RR using a Bell BGM-3 instrument system onboard the platform RV Roger Revelle

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743449
title: Karyotype diversity between species of Crenicichla (Perciformes, Cichlidae) from different Brazilian hydrographic basins

description: Abstract Crenicichla is the largest genus in the Cichlidae family in South America. The genus includes 100 valid species that are popularly known in Brazil as jacundás or joaninhas and are widely distributed in rivers east of the Andes. Cytogenetic analyses were carried out on seven species in this genus. All species showed a diploid number of 48 with interspecific differences in karyotype formulas and AgNORs located in interstitial position on the short arm of the largest metacentric pair, except for the two populations from C. britskii. Population A showed terminal markings on the long arm of the fifth pair of the complement, and population B showed up to two marked chromosome pairs. FISH with an 18S rDNA probe was coincident with AgNORs and CMA3, except for pair 6 from population B of C. britskii that did not presented positive CMA3 sites. This work presents first cytogenetic data for C. haroldoi, C. maculata, and C. punctata, and the results show karyotypic patterns similar to those in the literature. However, the diversity found in populations of C. britskii represents new information about the evolution of the karyotype of the Cichlidae family, which has been conservative. Furthermore, the data could assist in phylogenetic studies of Crenicichla.
DOI: 10.6084/m9.figshare.7743452.v1
title: Limnology of shallow lakes of the semi-arid Central Pampa of Argentina

description: Abstract Aim The Central Pampa of Argentina has three recognized phytogeographic regions that arise due to the decrease in rainfall towards the west. The area has numerous lakes that are mainly temporary, with hydroperiods that relate to climatic cycles, although some of them have changed due to anthropogenic influence. Some of these lakes have been studied with special reference to zooplankton, but information on their physical and chemical aspects is scarce. Consequently, managing and evaluating the anthropogenic effects on these ecosystems is challenging. The objective of this study was to explore the limnological characteristics of lakes in different regions in the Central Pampa that experience different anthropic influences. Methods Ten lakes were sampled seasonally (January, April, July, and October) during 2007. In situ measurements included transparency, pH, temperature, and dissolved oxygen concentration, and 2-L water samples were collected to determine salinity, ion composition, suspended solids, nutrient concentration, and phytoplankton chlorophyll- a concentration. Results Salinity ranged from 0.32-136.72 g L-1, with Na+ being the dominant ion in nine lakes and Cl- and HCO3- predominating in the higher- and lower-salinity lakes respectively. Nutrient concentrations were high (total Kjeldahl nitrogen: 7.97-34.69 mg L-1; total phosphorous: 4.07-14.82 mg L-1), and all lakes were hypertrophic. We determined three lake classes: i) lakes transformed from low-salinity lakes into hypersaline ones through human inactivation of the fluvial system that fed it; ii) mesosaline temporary lakes lacking fish, with low concentrations of chlorophyll-a and influenced by agricultural activities, and iii) subsaline and hyposaline lakes, highly modified by urban sewage, converted in permanent lakes (which allowed fish fauna development) and with reduced water transparency (due to high concentrations of phytoplankton chlorophyll-a). Conclusions The chemical diversity of the studied lakes is low, and their predominance of Na+ and Cl- indicated that evaporation and crystallization control the water chemistry. Additionally, this study showed the consequences of the anthropic impact, which alter water chemical composition, trophic structure and, thus, the ecological characteristics of lakes.
DOI: 10.6084/m9.figshare.7743440
title: Linkage disequilibrium and past effective population size in native Tunisian cattle

description: Abstract To carry out effective genome-wide association studies, information about linkage disequilibrium (LD) is essential. Here, we used medium-density SNP chips to provide estimates of LD in native Tunisian cattle. The two measures of LD that were used, mean r2 and D’, decreased from 0.26 to 0.05 and from 0.73 to 0.40, respectively, when the distance between markers increased from less than 20 Kb to 200 Kb. The decay in LD over physical distance occurred at a faster rate than that reported for European and other indigenous breeds, and reached background levels at less than 500 Kb distance. This is consistent with the absence of strong selective pressure within the Tunisian population and suggests that, in order to be effective, any potential genome-wide association mapping studies will need to use chips with higher marker density. An analysis of effective population size (Ne) based on LD data showed a decline in past Ne, with a sudden drop starting about eight generations ago. This finding, combined with the high levels of recent inbreeding revealed by runs of homozygosity (ROH) analysis, indicate that this population is endangered and may be in urgent need of a conservation plan that includes a well-designed genetic management program.