cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.x7dv8s
title: Occurrence Download

description: A dataset containing 634257 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Inventario Español de Especies Terrestres (MAGRAMA)"
 ]
}

 The dataset includes 634257 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002764-250214102907787/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.28428980
title: Supplementary Material for: Genetic and epigenetic insights into Werner Syndrome

description: Background: 
Werner syndrome is an autosomal recessive disorder characterized by premature aging and cancer predisposition, caused by loss of function mutations in WRN gene. To date, more than 70 different pathogenic variants have been identified across the WRN locus, with an increasing number of newly reported mutations. Even if the clinical phenotypes of WS seem to be indistinguishable among the different WRN mutation types, a certain genotype/phenotype correlation has been identified, especially regarding the predisposition to certain type of malignant disease. Along this line, the knowledge of the genetic aspects related to WRN is a fascinating land still object of intensive studies.

Summary and key messages: 
This review discusses both the genetics and epigenetic regulation of WRN gene, with a special focus on the pathogenic variants that have been identified in the WRN locus across different populations. Indeed, we think that investigating these aspects is the basis starting from which is it possible to depict WRN role in aging and cancer development processes, with the final goal of opening new perspectives for future therapeutic strategies directed to the treatment not only of this syndrome, for which, to date, there is no cure, but also of many types of malignant diseases and all those disturbs related to the physiological aging.
DOI: 10.6084/m9.figshare.28428983
title: Supplementary Material for: Stage I Non-Seminomatous Testicular Cancer. Long-term follow-up with surveillance approach post-orchiectomy.

description: Introduction: Adjuvant treatment for patients with stage I non-seminomatous germ cell tumors (NSGCT) could be active surveillance (AS), chemotherapy, or retro-peritoneal lymph node dissection (RLND). AS is the preferred option in most cas-es. The aim of this study was to evaluate long-term survival and prognostic factors in our population with AS approach.
Methods: We collected information from patients with stage I NSGCT of the testis in medical records from 1995 to 2016. Patients had negative serum tumor mark-ers and imaging of chest, abdomen, and pelvis with no evidence of metastasis. At relapse, if occurs, patients were treated with chemotherapy, surgery, or both. Kaplan-Meier method was used to estimate survival. Relationships with outcomes were analyzed using multivariable Cox regression and log-rank analysis.
Results: 457 patients were included. The median age at diagnosis was 25 years. The median follow-up was 65.3 meses (range 12 to 270 months). Relapses were detected in 92 (20%) patients with a median time to recurrence of 7.1 months (range 1.1 to 123 months). Retroperitoneal lymph nodes were the most common site of relapsed (41.3%), and most patients presented biochemical and imaging recurrence (67.4%). Vascular invasion (VI) was significantly associated with re-currence [HR 2.38 (CI 95%, 1.24-4.56), p=0.008] in the multivariate analysis, and rete testis invasion in the univariate analysis (p=0.027). After salvage treatment, 83 patients (91.1%) were disease-free. The overall survival was 98.25% at 20 years. 
Conclusions: AS is an effective non-adapted risk-based approach in patients with stage I NSGCT. Almost 100% are alive at 20 years. Nearly all relapses were cured with salvage therapy. Toxicity related to adjuvant treatments, as well as overtreatment, could be avoided.
DOI: 10.6084/m9.figshare.28428986
title: Supplementary Material for: The Association Between Tumor Necrosis Factor-Alpha Inhibitor Use and Dementia Risk in Patients with Rheumatoid Arthritis: A Systematic Review and Meta-analysis

description: Introduction: Tumor necrosis factor-alpha inhibitors (TNFi), commonly prescribed for rheumatoid arthritis (RA), have been studied for their potential association with dementia risk. However, previous findings are inconclusive. This study aims to evaluate the impact of TNFi use on dementia in patients with RA.
Methods: Systematical search of MEDLINE, Embase, and CENTRAL databases from inception to October 1, 2023, was conducted. Longitudinal comparative studies investigating the association between TNFi use and risk of dementia in patients with RA were included. Pooled adjusted risks of dementia, and meta-analysis conducted to synthesize relative estimates with 95% confidence intervals (CIs). 
Results: Seven observational studies involving 633,089 patients with RA were included, of which six were included in the meta-analysis. The pooled meta-analysis comparing the risk of dementia (hazard ratio [HR] = 0.77, 95% CI 0.64–0.93) and Alzheimer's disease (AD) (odds ratio = 0.31, 95% CI 0.23–0.43) between TNFi users and non-users showed a significant association. However, the pooled HR for AD risk was inconsistent. Also, the subgroup analyses indicated that TNFi use was associated with a decreased dementia risk in older adult patients with a mean age of ≥65 years at enrollment (HR = 0.86, 95% CI 0.80-0.92) and in TNFi new users (HR = 0.86, 95% CI 0.80-0.92).
Conclusions: Systematic review and meta-analysis suggest that lowering the level of systemic TNF-alpha by using TNFi could lower the risk of dementia. However, given the retrospective nature of the included studies, further prospective studies are needed to evaluate the role of TNFi in dementia onset.
DOI: 10.6084/m9.figshare.28428989.v1
title: Supplementary Material for: Impact of the COVID-19 pandemic on psychosocial distress in adolescents with obesity compared to those with type1 diabetes: Results from the KICK-COVID Study in Germany

description: Introduction: The aim of this study is to investigate the impact of the COVID-19 pandemic on psychosocial well-being in adolescents with obesity compared to those with type 1 diabetes. 
Methods: As part of the German KICK-COVID Study, adolescents aged 12-21 with overweight or obesity from the German/Austrian Adiposity-Follow-up-Registry (APV) completed well-being, anxiety, and depression questionnaires (WHO-5; GAD-7; PHQ-9) during routine visits amidst the COVID-19 pandemic. By multivariable linear regression models, adjusted for age, gender, and immigration background, the association between psychosocial distress, anthropometrics, and cardiometabolic risk factors was analysed. Data were compared to those of youth with type 1 diabetes from the German/Austrian Diabetes-Follow-up-Registry (DPV) and normative values from the general population. Additionally, a mediation analysis examined the impact of loneliness on mental health through media consumption. 
Results: From June 2021 to September 2023, 235 adolescents from 6 German and 1 Austrian paediatric obesity centres were enrolled. Results were compared to 235 age- and gender-matched participants from the DPV registry (54.04% males; mean age 15.21±1.66 years) and normative values. Youth with type-1 diabetes were more anxious about their health risk, but distress factors were more pronounced in the APV group (p&lt;0.001). Girls from the APV group showed higher mental distress than boys across all applied questionnaires, but not for age, BMI-SDS, and migration background as predictors. Perception of loneliness correlated with poorer mental health outcomes, but it was not associated to media consumption. Comparisons with normative values revealed significantly higher depression and anxiety scores (p&lt;0.001) and lower well-being scores in the APV group (p&lt;0.01). 
Conclusions: Youth with obesity and diabetes experienced significant psychosocial distress during the COVID-19 pandemic. Disease-specific differences were observed on the level of single items: Adolescents with type 1 diabetes expressed heightened concern about their health risks, while those with obesity reported lower self-esteem, increased suicidal thoughts, and fluctuating appetite. Female gender appeared to pose an additional risk factor. Media consumption was notably higher in the APV cohort. Healthcare providers should be vigilant regarding psychological comorbidities in youth with chronic conditions, particularly during periods of heightened stress.
DOI: 10.6084/m9.figshare.28428995
title: Supplementary Material for: Effects of ophthalmic medications on conjunctival goblet cell density in New Zealand white rabbits.

description: Introduction: The conjunctiva contains numerous specialized cells called conjunctival goblet cells (CGCs). The topical application of specific eye drops to the ocular surface has conclusively been linked to cause a reduction in CGCs, a condition which has been associated with dry eye and other ocular surface disorders. The purpose of this study was to assess if the use of benzalkonium chloride as a preservative in common ophthalmic medications affects CGCs density in New Zealand White (NZW) rabbit conjunctivas. Methods: Data from seven preclinical studies conducted between March 2016 and April 2021 were analyzed, involving 146 male New Zealand White (NZW) rabbits aged 2 to 3 months. Prior to study participation, rabbits underwent a seven-day quarantine period in individual housing, during which their general health was monitored. Rabbits had ad libitum access to water and food, with intake data recorded. Comprehensive ophthalmological examinations were performed on all eyes prior to and throughout the studies. The density of corneal endothelial cells (CGCs) was specifically assessed using AB/PAS staining and quantified with a high-power (40X) field objective (Ocular 18x22), expressed as a percentage relative to epithelial cells. Results: No statistically significant differences were found between the with-BAK group and without-BAK group. The mean density in the 30-day group presented a statistically significant higher density than the &gt;30-day group (p=0.005). Analysis of the treatment revealed that antibiotic/steroid combination group had a higher average number of CGCs compared to both the antihistaminic group (p=0.004) and hypotensive agent group (p=0.047). Conclusion: Exposure to BAK-preserved medications for 30 days results in a higher density of CGCs compared to prolonged exposure to BAK-preserved medications exceeding 30 days. The pharmacological effects and associated cellular damage induced by BAK vary depending on the specific medication with which it is combined.
DOI: 10.6084/m9.figshare.28428998.v1
title: Supplementary Material for: Surgical Preferences and Fears: Misconceptions About Robotic-Assisted Surgery

description: Introduction and objective
Robotic-assisted surgery (RAS) is widely adopted across surgical fields, notably urology, but patient knowledge remains limited, often shaped by misconceptions. Previous research indicates factors like age, profession, and technology use influence RAS perceptions. This study investigates public knowledge, preferences, and misconceptions about RAS within a German cohort.
Methods
A cross-sectional survey at a university hospital’s open house gathered responses from 339 participants prior to an RAS exhibition. The questionnaire assessed demographics, surgical preferences, and RAS knowledge. Statistical analyses, including t-tests, chi-squared tests, ANOVA, and multivariate logistic regression, identified key associations.
Results
Results showed 71% (234) of participants favored RAS over conventional surgery, yet misconceptions persisted in 38% (122), particularly among pensioners (48% (46) , p &lt; 0.01). Misconceptions were linked to a preference for conventional surgery (43% (52) vs. 19% (36), p &lt; 0.01). Surgical preference emerged as a significant predictor of misconception. Concerns included surgeon skill (41%, 141) and machine malfunction (39%, 132), with younger participants fearing human error and older individuals fearing technical failure (p &lt; 0.01).
Conclusion
This local study reveals strong public support for RAS but underscores prevalent misconceptions, especially among older adults, suggesting that addressing misconceptions could foster acceptance and informed decision-making.
DOI: 10.6084/m9.figshare.28429007.v1
title: Supplementary Material for: The Long-Term Behavioural Effects of Maternal Creatine Supplementation in a Spiny Mouse Model of Birth Asphyxia

description: Introduction: Birth asphyxia-induced encephalopathy is a major cause of long-term neurological morbidity, including cognitive and motor deficits. A proposed treatment is maternal creatine supplementation for prophylactic neuroprotection. This study examined how maternal creatine supplementation with or without birth asphyxia affected the behaviour of spiny mice offspring. 

Methods: On day 20 of gestation (mid-gestation; term=39 days), dams were randomly allocated to either a daily diet containing 5% w/w creatine monohydrate or remained on standard rodent chow. On gestational day 38, dams underwent either control caesarean section where offspring were delivered and recovered immediately, or birth asphyxia whereby the pregnant uterus was excised and placed in a saline bath for 7.5 min, inducing global hypoxia. All offspring were then cross-fostered to a lactating dam. Behavioural assessments were then completed on recovered offspring from neonatal to adolescent/adult ages (postnatal day [PND]3–41) using the open-field, elevated plus maze and novel object recognition test was conducted. 

Results: Offspring that underwent birth asphyxia displayed locomotor deficits and increased anxiety-like behaviour at PND3-7 in the open-field test (p&lt;0.05) and impaired novel object discrimination at PND18 (p&lt;0.05). Antenatal creatine exposure reduced anxiety-like behaviour irrespective of asphyxia in pups at PND3, indicating an amelioration of the asphyxia-induced anxiety-like behaviour. In adolescence/adulthood, creatine and asphyxia-exposed offspring showed reduced object exploration (p&lt;0.0001). Antenatal creatine led to sustained reductions in anxiety-like behaviour in the elevated plus maze at adolescence and increased body weight, regardless of birth asphyxia exposure (p&lt;0.05). 

Conclusion: Antenatal creatine exposure following maternal dietary creatine supplementation decreased anxiety-like behaviour in spiny mice offspring. This change negated behavioural abnormalities caused by birth asphyxia in the neonatal period, though it may have broader influences on long-term emotional and information processing in offspring which warrants further investigation.
DOI: 10.6084/m9.figshare.28429010.v1
title: Supplementary Material for: Adjunctive active vitamin D (AVD) decreases kidney function during treatment of secondary hyperparathyroidism (SHPT) with extended-release calcifediol (ERC) in non-dialysis chronic kidney disease (ND-CKD)

description: Introduction: Sustained 30% reductions of intact parathyroid hormone (iPTH) with ERC are associated with slower decline in estimated glomerular filtration rate (eGFR) in ND-CKD patients with SHPT. Such iPTH reductions usually require elevation of serum total 25-hydroxyvitamin D (25D) to ≥50 ng/mL, but achieving these reductions can be limited by the ERC dose ceiling (60 μg/day), raising the question of whether adjunctive (adj) AVD might be appropriate to further reduce iPTH.

Methods: This randomized controlled trial (RCT) examined whether adj AVD could safely increase iPTH reductions achieved with ERC and further reduce the rate of eGFR decline in 78 ND-CKD adults treated with ERC for 38 weeks. Participants had mean age of 66 years, body mass index of 35 kg/m2, 41% were female, 63% White, 36% Black, 19% Hispanic. At ERC initiation, participants had plasma iPTH 85-&lt;500 pg/mL, eGFR 15-&lt;60 mL/min/1.73 m2, serum 25D 10-&lt;30 ng/mL, corrected serum calcium (Ca) 8.4-&lt;9.8 mg/dL, serum phosphorus (P) 2.0-&lt;5.0 mg/dL, and absence of macroalbuminuria (&gt;300 mg/g creatinine). At baseline (BL; week 38), participants had plasma iPTH &gt;70 pg/mL and serum Ca &lt;9.8 mg/dL and were randomized 3:1:1:1 to daily ERC (60 μg) for 14 additional weeks with (n=40) or without (n=38) adj daily oral calcitriol (0.25 μg), doxercalciferol (0.5 μg) or paricalcitol (1.0 μg). Measurements of eGFR, iPTH, 25D, Ca, P and fibroblast growth factor 23 (FGF23) were obtained at BL and through end of treatment (EOT). 

Results: No significant intergroup differences were observed at BL. Mean 25D at BL was 65 ng/mL and rose 14 ng/mL by EOT in both groups (p&lt;0.001). Mean BL iPTH was 137 pg/mL and fell by a further 35.4% (p&lt;0.001) with adj AVD therapy versus 2.2% without. Mean Ca, P and FGF23 increased with adj AVD by 0.40 mg/dL (p&lt;0.001), 0.27 mg/dL (p&lt;0.01) and 49.1 pg/mL (155%; p&lt;0.001), respectively, but remained unchanged with ERC alone. Mean BL eGFR was 25.4 mL/min/1.73m2 and fell by 11.8% (p&lt;0.05) with adj AVD versus 3.0% without. 

Conclusion: Adj AVD at these doses enabled 35% more iPTH reduction in ND-CKD patients with mild to moderate SHPT on long-term ERC treatment but increased mean serum Ca and P by 0.40 and 0.27 mg/dL, respectively, FGF23 by more than 2-fold and eGFR decline by 4-fold, suggesting that adding AVD to ERC has untoward effects that override the nephrosparing impact of iPTH reductions with ERC treatment alone. Corroboration is warranted with a larger, longer RCT.
DOI: 10.6084/m9.figshare.28429013.v1
title: Supplementary Material for: Development and validation of a Japanese outcome tool for perceptual assessment of speech in patients with cleft palate

description: Introduction: The lack of a standardized evaluation method for cleft palate speech makes it difficult to exchange information at the domestic and global levels. We aimed to develop and validate a Japanese outcome tool for the perceptual assessment of speech in patients with cleft palate.
Methods: The tool we developed was based on the Cleft Audit Protocol for Speech-Augmented (CAPS-A) with modifications to some speech parameters. We developed Japanese speech samples and parameters for the Japanese Cleft Speech Outcome Tool (CAPS-A-Japan: CAPS-A-JP). This study was conducted in the Japancleft speech working group at the Japanese Cleft Palate Association. In Phase 1, we constructed and developed the speech sampling protocol. In Phase 2, After the revision of the definitions and criteria for several cleft speech characteristics and the speech sample, a total of 20 audio recordings were tested. In Phase 3, criterion validity was assessed by comparing the CAPS-A-JP resonance outcomes reported for 40 cases with the outcomes of the clinical investigations. In Phase 4, a listening experiment with six Japanese speech-language-hearing therapists was conducted twice to test the intra- and inter-rater reliabilities for 20 cases. Intra-class correlation coefficients (ICCs) were used to analyze reliability.
Results: The mean percentage agreement on criterion validity for resonance was 71% on high-pressure sentences but 54% on low-pressure sentences. The inter-rater reliability was rated moderate/good (ICC: 0.45–0.80), except for developmental immaturities/errors that were rated fair. The ICC was very low or incalculable for hyponasality and syllable error patterns because there were few cases of hyponasality and syllable errors. The intra-rater reliability was rated as good/very good. The ICC was very low or incalculable in hyponasality, nasal emission, and syllable error patterns.
Conclusions: The standardized outcome tool, CAPS-A-JP, was developed for the first time in Japan. Future research is required to optimize this tool based on the inclusion of a training protocol.
DOI: 10.6084/m9.figshare.28429016.v1
title: Supplementary Material for: Behavior Risk Factors for Non-Communicable Diseases and Caries in Adolescents: A Population Study

description: Introduction: Caries is the most widespread non-communicable disease (NCD) globally and can predict fatality NCDs in the future, such as diabetes and cardiovascular diseases. Therefore, we hypothesized that the behavioral risk factors for those NCDs may be present in adolescents with caries. Objective: To analyze the association of unhealthy diet, tabagism, and alcohol with caries in adolescents. Methods: This population-based study with a cross-sectional design used data from 2515 adolescents aged 18-19 yo, born in São Luís-MA, Brazil, and part of the RPS Cohort Consortium. The exposures were the latent variables of the Unhealthy Diet Pattern (sugars, fast foods, and salty snacks) and Addictive Behaviors (tabagism and risk of alcohol dependence). The outcome was the number of decayed teeth. The model adjustment considered the Socioeconomic Status (head of household education, adolescent education, economic class, and monthly family income) as a distal determinant and the oral biofilm as a mediator between the exposures and the outcome, analyzed by Structural Equation Modeling (SEM), using the Mplus 8.0 software. Results: Unhealthy Diet Pattern was associated with a higher number of decayed teeth directly (SC=0.092, p≤0.001) and indirectly via dental biofilm (SC=0.020, p≤0.001). Addictive Behaviors were associated with a higher number of decayed teeth indirectly via dental biofilm (SC=0.019, p=0.013). The Unhealthy Diet Pattern and Addictive Behaviors were associated with each other (CP=0,199; p&lt;0,001). Conclusion: Besides sugars, other behavioral risk factors for NCDs underlie caries, emphasizing the importance of comprehensive adolescent health care.
DOI: 10.6084/m9.figshare.28429019
title: Supplementary Material for: Sex Differences for Social Determinants Associated with Lewy Body Dementia Onset and Diagnosis

description: Introduction: Multiple studies report sex and gender differences in Lewy body dementia (LBD); however, there is a paucity of research investigating social determinants associated with LBD. 

Methods: Participants with LBD (51 females, 79 males) and controls with similar age (64 females, 60 males) completed remote surveys assessing various social and demographic variables, and age at LBD onset for LBD group. Sex-stratified comparisons for LBD and control groups; comparisons of females and males with LBD were done for social determinants. Sex differences for onset age were further analyzed with linear models adjusting for significantly differing social variables between the sexes.

Results: LBD group had lower years of education, income, subjective social status than controls; with larger differences for males than females (p&lt;.05 for all). Higher percentage of females with LBD were living alone (p=.016) and not married/partnered (p=.002) compared to males with LBD. Adjusting for social variables that differed between the sexes, females were younger at cognitive impairment onset (p=.037) and diagnosis (p=.032). For the overall cohort, being ethnoracially minoritized, sexual and gender minoritized, and having lower education quality were associated with younger age at symptom onset (p&lt;.049 for all). For females, lower childhood subjective social status (p=.037); for males, being ethnoracially minoritized (p&lt;.001) and lower years of education (p=.031) were associated with younger age at diagnosis.

Conclusion: Social determinants, even during childhood can impact the LBD onset differently for females and males. Interactions between biological and social factors need to be investigated further with inclusive and diverse cohorts in LBD.