cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.dbrv15f4t
title: Data for: Mercury contamination challenges the behavioral response of a keystone species to Arctic climate change

description: Combined effects of multiple, climate change-associated stressors are of
 mounting concern, especially in Arctic ecosystems. Elevated mercury (Hg)
 exposure in Arctic animals could affect behavioural responses to changes
 in foraging landscapes linked to climate change, generating interactive
 effects on behaviour and population resilience. We investigated this
 hypothesis in the little auk (Alle alle), a keystone Arctic seabird. We
 compiled behavioural data using accelerometers, and quantified blood
 mercury and environmental conditions (sea surface temperature (SST), sea
 ice coverage (SIC)) across multiple years. Warm SST and low SIC reshaped
 time activity budgets (TABs) and diving patterns, causing decreased
 resting, increased flight, and longer dives. Mercury contamination was not
 associated with TABs. However, highly contaminated birds lengthened
 inter-dive breaks when making long dives, suggesting mercury-induced
 physiological limitations. As dive durations increased with warm
 SST, subtle toxicological effects threaten to increasingly
 constrain diving and foraging efficiency as climate change progresses,
 with ecosystem-wide repercussions.
description: We used triaxial accelerometers to collect detailed behavioural
 records from little auks (<em>Alle alle</em>) at Ukaleqarteq,
 East Greenland, during the chick rearing period (July–August) of 2017 to
 2021, and linked this information to environmental conditions and blood
 mercury (Hg) contamination. Environmental conditions considered were sea
 surface temperature (SST) and sea ice coverage (SIC), which have major
 influences on the favourability of foraging conditions for little auks. We
 determined time activity budgets (TABs) and behavioural performance traits
 (dive and inter-dive durations, flight durations) from accelerometer data
 using custom functions in Igor Pro, as described in full in our
 manuscript. 
description: Datasets can be opened in Excel and R.
DOI: 10.5061/dryad.5hqbzkh99
title: Phylogenetic revision of Dennstaedtioideae (Dennstaedtiaceae: Polypodiales) with description of Mucura , gen. nov.

description: We undertook a molecular phylogenetic revision of hayscented ferns
 (Dennstaedtiaceae: Dennstaedtioideae) using four plastid markers. Our
 sampling represents ca. 40% of the extant diversity and includes the type
 species for each of the relevant segregate genera. We coded 20 discrete
 morphological characters which we used to find diagnosable clades. We show
 that Dennstaedtia is polyphyletic, with the majority of species forming
 three morphologically distinct clades, but its type (D.flaccida) is nested
 within Microlepia. As such, we support the conservation of
 Dennstaedtiawith a new type, D.dissecta. Following our results, we develop
 a classification of four genera: Dennstaedtia, Microlepia, Mucura (gen.
 nov.), and Sitobolium. Beyond the inclusion of D.flaccida, we propose to
 maintain Microlepia with its current circumscription. Except for a single
 adventive species in the Neotropics, Microlepia is a Paleotropical genus
 of about 60 species diagnosed by their distinctive perispore ornamentation
 of rodlets, and by petioles that lack epipetiolar buds. Mucura is a
 Neotropical genus of two species that differ from all other
 Dennstaedtiaceae by the combination of dichotomously branching rhizomes,
 petioles that lack epipetiolar buds, marginal sori with both abaxial and
 adaxial indusia, and trilete spores with a unique perispore ornamentation.
 As defined here, Dennstaedtia is a pantropical genus of about 55 species
 recognized by having unbranched rhizomes, petioles bearing epipetiolar
 buds, and by often bearing proliferous buds upon the leaves. Sitobolium is
 a small clade of ca. five species distinguished by their relatively small
 leaves that have elongate catenate hairs. These hairs often bear a
 capitate non-glandular terminal cell. In support of our classification, we
 provide a key to the eleven genera of Dennstaedtiaceae, and for the four
 genera of Dennstaedtioideae we provide morphological and geographic
 synopses, a list of constituent species, and necessary new combinations.
description: Nucleotide alignment of plastid markers rbcL, rpl16, rps4-trnS,
 and trnL-trnF
DOI: 10.5281/zenodo.17550554
title: Forward Head Posture in individuals with and without chronic neck pain

description:  ABSTRACT

Background: Chronic neck pain (CNP) is a leading cause of disability worldwide. Forward head posture (FHP) is a prevalent craniocervical posture, with controversy regarding its relationship with CNP. The craniovertebral angle (CVA) is commonly used to assess FHP.

Objectives: to determine whether significant differences in head posture, assessed through the CVA, exist between adults with and without CNP. Secondary objectives include conducting an exploratory analysis of variables, evaluating intra- and interobserver reliability of CVA by novice evaluators and analyzing CVA correlations between sitting and standing.

Design: Two-group cross-sectional study.

Methods: 92 participants were evaluated (50 with CNP, 42 asymptomatic). CVA was measured in sitting and standing using photogrammetry. Sociodemographic data, musculoskeletal pain prevalence, pain intensity, general health, physical activity, and neck disability were collected. CVA intra- and interobserver reliability was assessed using Kinovea. Factors associated with CVA were identified using multiple lineal regression analysis.

Results: No association was found between FHP and CNP. Gender and BMI were associated with CVA. Women had significantly lower CVA than men seated (B= -2.5, p= 0.024) and standing (B= -2.0, p = 0.031). Overweight individuals had significantly lower CVA than normal-weight individuals seated and standing (B= -0.6, p < 0.001 for both). Inter- and intra-reliability in both positions was excellent (all ICC > 0.90). A correlation was observed between sitting and standing measurements.

Conclusion: No significant association was found between CNP and FHP based on CVA. Sex, age and BMI demonstrated a substantial influence on head posture. CVA photogrammetry is reliable for novice evaluators.
DOI: 10.5281/zenodo.17550555
title: Forward Head Posture in individuals with and without chronic neck pain

description:  ABSTRACT

Background: Chronic neck pain (CNP) is a leading cause of disability worldwide. Forward head posture (FHP) is a prevalent craniocervical posture, with controversy regarding its relationship with CNP. The craniovertebral angle (CVA) is commonly used to assess FHP.

Objectives: to determine whether significant differences in head posture, assessed through the CVA, exist between adults with and without CNP. Secondary objectives include conducting an exploratory analysis of variables, evaluating intra- and interobserver reliability of CVA by novice evaluators and analyzing CVA correlations between sitting and standing.

Design: Two-group cross-sectional study.

Methods: 92 participants were evaluated (50 with CNP, 42 asymptomatic). CVA was measured in sitting and standing using photogrammetry. Sociodemographic data, musculoskeletal pain prevalence, pain intensity, general health, physical activity, and neck disability were collected. CVA intra- and interobserver reliability was assessed using Kinovea. Factors associated with CVA were identified using multiple lineal regression analysis.

Results: No association was found between FHP and CNP. Gender and BMI were associated with CVA. Women had significantly lower CVA than men seated (B= -2.5, p= 0.024) and standing (B= -2.0, p = 0.031). Overweight individuals had significantly lower CVA than normal-weight individuals seated and standing (B= -0.6, p < 0.001 for both). Inter- and intra-reliability in both positions was excellent (all ICC > 0.90). A correlation was observed between sitting and standing measurements.

Conclusion: No significant association was found between CNP and FHP based on CVA. Sex, age and BMI demonstrated a substantial influence on head posture. CVA photogrammetry is reliable for novice evaluators.
DOI: 10.5061/dryad.08kprr55z
title: Data from: A k-mer-based approach for phylogenetic classification of taxa in environmental genomic data

description: In the age of genome sequencing, whole genome data is readily and
 frequently generated, leading to a wealth of new information that can be
 used to advance various fields of research. New approaches, such as
 alignment-free phylogenetic methods that utilize k-mer-based distance
 scoring, are becoming increasingly popular given their ability to rapidly
 generate phylogenetic information from whole genome data. However, these
 methods have not yet been tested using environmental data, which often
 tends to be highly fragmented and incomplete. Here we compare the results
 of one alignment-free approach (which utilizes the D­2 statistic) to
 traditional multi-gene maximum likelihood trees in three algal groups that
 have high-quality genome data available. In addition, we simulate
 lower-quality, fragmented genome data using these algae to test method
 robustness to genome quality and completeness. Finally, we apply the
 alignment-free approach to environmental metagenome assembled genome data
 of unclassified Saccharibacteria and Trebouxiophyte algae, and single-cell
 amplified data from uncultured marine stramenopiles to demonstrate its
 utility with real datasets. We find that in all instances, the
 alignment-free method produces phylogenies that are comparable, and often
 more informative, than those created using the traditional multi-gene
 approach. The k-mer-based method performs well even when there is
 significant missing data, that includes marker genes traditionally used
 for tree reconstruction. Our results demonstrate the value of
 alignment-free approaches for classifying novel, often cryptic or rare,
 species, that may not be culturable or are difficult to access using
 single-cell methods but fill important gaps in the tree of life.
DOI: 10.15468/dl.56ayj3
title: Occurrence Download

description: A dataset containing 1014 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Germany",
 "TaxonKey is Habrophlebia lauta"
 ]
}

 The dataset includes 1014 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003358-260126135527185/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5061/dryad.12jm63z25
title: Bird-building collisions increase with weather conditions that favor nocturnal migration and with inclement and changing weather

description: Collisions with building windows are a top bird mortality source, but few
 studies have evaluated how bird-window collisions are influenced by the
 weather. By monitoring collisions daily at 21 buildings in Minneapolis,
 Minnesota, over 4 migration seasons, we show that weather influences the
 number of window collisions of nocturnal migrants in spring and fall,
 indicating that collisions may be forecastable based on weather
 conditions. Collisions increased with weather favoring migration, such as
 consecutive nights of south wind in spring and even short periods of north
 wind in fall. We also found evidence that spring and fall collisions
 increase with weather changes that impede migration, such as changes from
 fair conditions and tailwinds early in the night to headwinds near
 sunrise. Our study suggests complex weather effects never before
 considered in the context of bird collisions, including possible time lag
 effects of conditions two and three nights before collisions occur,
 effects of multi-day sequences of conditions, and interactions between
 conditions at different times of the night. More research is needed to
 determine if the accuracy of weather-based collision prediction systems
 improves by integrating such nuances and to clarify mechanisms through
 which these complex effects operate, such as influences of weather on
 migration intensity and collision avoidance behavior. Weather-based
 forecasts may allow refinement of collision mitigation approaches (e.g.,
 reducing building lighting on certain nights or using temporary glass
 coverings or treatments). However, because challenges remain to
 communicating such temporally targeted actions and implementing them in a
 timely manner, other bird-friendly practices (e.g., season-long lighting
 reduction and permanent glass treatments) should continue to be
 prioritized.
DOI: 10.5061/dryad.0vt4b8h35
title: Abdominal vagus nerve stimulation alleviates collagen-induced arthritis in rats

description: Rheumatoid arthritis (RA) is a chronic, autoimmune inflammatory disease.
 Despite therapeutic advances, a significant proportion of RA patients are
 resistant to pharmacological treatment. Stimulation of the cervical vagus
 nerve is a promising alternative bioelectric neuromodulation therapeutic
 approach. However, recent clinical trials show cervical vagus nerve
 stimulation (VNS) was not effective in a significant proportion of
 drug-resistant RA patients. Here we aim to assess if abdominal vagus nerve
 stimulation reduces disease severity in a collagen-induced arthritis (CIA)
 rat model. The abdominal vagus nerve of female Dark Agouti rats was
 implanted and CIA-induced using collagen type II injection. VNS (1.6 mA,
 200 µs pulse width, 50 µs interphase gap, 27 Hz frequency) was applied to
 awake freely moving rats for 3 h/day (days 11–17). At 17 days following
 the collagen injection, unstimulated CIA rats (n = 8) had significantly
 worse disease activity index, tumour necrosis factor-alpha (TNF) and
 receptor activator of NFkB ligand (RANKL) levels, synovitis and cartilage
 damage than normal rats (n = 8, Kruskal-Wallis: P &lt; 0.05). However,
 stimulated CIA rats (n = 5-6) had significantly decreased inflammatory
 scores and ankle swelling (Kruskal-Wallis: P &lt; 0.05) compared to
 unstimulated CIA rats (n = 8). Levels of tumour necrosis factor-alpha
 (TNFα) remained at undetectable levels in stimulated CIA rats while levels
 of receptor activator of NFkB ligand (RANKL) were significantly less in
 stimulated CIA rats compared to unstimulated CIA rats (P &lt; 0.05).
 Histopathological score of inflammation and cartilage loss in stimulated
 CIA rats were no different from that of normal (P &gt; 0.05). In
 conclusion, abdominal VNS alleviates CIA and could be a promising therapy
 for patients with RA.
description: Microsoft Excel and Prism GraphPad
DOI: 10.5061/dryad.0vt4b8h36
title: Data from: Fast drug rotation reduces bacterial resistance evolution in a microcosm experiment

description: Drug rotation (cycling), in which multiple drugs are administrated
 alternatively, has the potential for limiting resistance evolution in
 pathogens. The frequency of drug alternation could be a major factor to
 determine the effectiveness of drug rotation. Drug rotation practices
 often have low frequency of drug alternation, with an expectation of
 resistance reversion. Here we, based on evolutionary rescue and
 compensatory evolution theories, suggest that fast drug rotation can limit
 resistance evolution in the first place. This is because fast drug
 rotation would give little time for the evolutionarily rescued populations
 to recover in population size and genetic diversity, and thus decrease the
 chance of future evolutionary rescue under alternate environmental
 stresses. We experimentally tested this hypothesis using the
 bacterium Pseudomonas fluorescens and two antibiotics
 (chloramphenicol and rifampin). Increasing drug rotation frequency reduced
 the chance of evolutionary rescue, and most of the final surviving
 bacterial populations are resistant to both drugs. Drug resistance
 incurred significant fitness costs, which did not differ among the drug
 treatment histories. A link between population sizes during the early
 stages of drug treatment and the end-point fates of populations
 (extinction versus survival) suggested that population size recovery and
 compensatory evolution before drug shift increase the chance of population
 survival. Our results therefore advocate fast drug rotation as a promising
 approach to reduce bacterial resistance evolution, which in particular
 could be a substitute for drug mixing when the latter has safety risks.
description: This dataset was collected from the experiment we performed. See
 the article for details.
DOI: 10.5061/dryad.c866t1g9x
title: Does pre-sorting by colour using visible and high-energy violet light improve the detection of plant species in honey bee pollen baskets?

description: Premise Pollen collected by honey bees from different plant species often
 differs in color, and this has been used as a basis for plant
 identification. The objective of this study was to develop a new, low-cost
 protocol to sort pollen pellets by color using high-energy violet light
 and visible light to determine whether pollen pellet color is associated
 with variations in plant species identity. Methods and Results We
 identified 35 distinct colors and found that 52% of pollen subsamples
 (n = 200) were dominated by a single taxon. Among these near-pure pellets,
 only one color consistently represented a single pollen taxon (Asteraceae:
 Cichorioideae). Across the spectrum of colors spanning yellows, oranges,
 and browns, similarly colored pollen pellets contained pollen from
 multiple plant families ranging from two to 13 families per color.
 Conclusions Sorting pollen pellets illuminated under high-energy violet
 light lit from four directions within a custom-made light box aided in
 distinguishing pellet composition, especially in pellets within the same
 color.
description: <em>Apis mellifera</em> pollen pellets were sorted by
 colour to investigate whether pollen pellet colour correlates to
 palynological identity. Pollen pellets were collected using hive traps,
 then dried in a dehydrator. Hive samples were sorted by colour, and three
 pellets from each colour group were acetolysed and mounted on slides.
 Palynological identity was assigned after viewing slides through a
 compound microscope. <br><br>Dataset includes sample number,
 subsample (colour group) number, palynological identity (e.g., family,
 subfamily, genus), pollen count, pollen volume, and pollen proportion per
 sample.
description: Microsoft Excel
DOI: 10.5061/dryad.tmpg4f52k
title: Data from: A surface acoustic wave (SAW)-based lab-on-chip for the detection of active α-glycosidase

description: Enzyme detection in liquid samples is a complex laboratory procedure,
 based on assays that are generally time- and cost-consuming, and require
 specialized personnel. Surface acoustic wave sensors can be used for this
 application, overcoming the cited limitations. To give our contribution,
 in this work we present the bottom-up development of a surface acoustic
 wave biosensor to detect active α-glycosidase in aqueous solutions. Our
 device, optimized to work at an ultra-high frequency (around 740 MHz), is
 functionalized with a newly synthesized probe
 7-mercapto-1-eptyl-D-maltoside, bringing one maltoside terminal moiety.
 The probe is designed ad hoc for this application and tested in-cuvette to
 analyze the enzymatic conversion kinetics at different times, temperatures
 and enzyme concentrations. Preliminary data are used to optimize the
 detection protocol with the SAW device. In around 60 min, the SAW device
 is able to detect the enzymatic conversion of the maltoside unit into
 glucose in the presence of the active enzyme. We obtained successful
 α-glycosidase detection in the concentration range 0.15–150 U/mL, with an
 increasing signal in the range up to 15 U/mL. We also checked the sensor
 performance in the presence of an enzyme inhibitor as a control test, with
 a signal decrease of 80% in the presence of the inhibitor. The results
 demonstrate the synergic effect of our SAW Lab-on-a-Chip and probe design
 as a valid alternative to conventional laboratory tests.
description: UV data were acquired via UV-Vis spectroscopy with a JASCO V550
 spectrophotometer (JASCO Europe, Cremello, Italy); the raw data were used
 without any further processing. QCM data were acquired
 with a QCM-D E4 model (Q-Sense AB, Sweden); the raw data were processed by
 MATLAB to eliminate any offset or drift. SAW-LoC data
 were acquired with a vectorial network analyzer E5071C (Agilent
 Technologies) connected to an RF switch 34980A (Agilent Technologies); an
 in-house software based on LabView® is used to pilot the RF-switch and the
 vectorial network analyzer.
description: Data files are uploaded as Plain text or as .csv files. The .csv
 files can be opened with Microsoft Excel or Google Sheets.
DOI: 10.5061/dryad.bnzs7h4fb
title: Testing the predictability of morphological evolution in contrasting thermal environments

description: Gaining the ability to predict population responses to climate change is a
 pressing concern. Using a ‘natural experiment’, we show that testing for
 divergent evolution in wild populations from contrasting thermal
 environments provides a powerful approach, and likely an enhanced
 predictive power for responses to climate change. Specifically, we used a
 unique study system in Iceland, where freshwater populations of threespine
 sticklebacks (Gasterosteus aculeatus) are found in waters warmed by
 geothermal activity, adjacent to populations in ambient-temperature water.
 We focused on morphological traits across six pairs from warm and cold
 habitats. We found that fish from warm habitats tended to have a deeper
 mid-body, a sub-terminally orientated jaw, steeper craniofacial profile,
 and deeper caudal region relative to fish from cold habitats. Our common
 garden experiment showed that most of these differences were heritable.
 Population age did not appear to influence the magnitude or type of
 thermal divergence, but similar types of divergence between thermal
 habitats were more prevalent across allopatric than sympatric population
 pairs. These findings suggest that morphological divergence in response to
 thermal habitat, despite being relatively complex and multivariate, are
 predictable to a degree. Our data also suggests that the potential for
 migration of individuals between different thermal habitats may enhance
 non-parallel evolution and reduce our ability to predict responses to
 climate change.
description: The data is comprised of 2d landmark data from the morphology of
 stickleback (Gasterosteus aculeatus). These were collected from digital
 photos taken of individuals derived from wild/field sites, as well as F1
 individuals reared under common conditions in the lab. 
 Data comprised of linear distances were also collected from
 digital photos. Pythagoras theorem was used to calculate a distance
 between landmarks to gather spine length, and pector fin
 lengths.  All landmark data were collected using the
 software tpsDig2. We provide linear distances as they were calculated,
 while landmark data are provided as coordinates in .tps forma with a file
 for accompanying variables for centroid size (cs), population (pop),
 population pair (pair), lab or wild derivation, thermal habitat (temp),
 and whether the population was found in sympatry or allopatry with respect
 to thermal habitat (allosym).
description: We recommend the use of the geomorph package in R for the
 analysis of 2 morphometric data.