cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14326778
title: Fig 2 source data.xlsxCritical roles for the LINC01119-SOCS5 axis as a novel theranostic in triple-negative breast cancersThe LINC01119-SOCS5 axis as a critical theranostic in triple-negative breast cancer

description: The development of triple-negative breast cancer (TNBC) is critically regulated by certain tumor-microenvironment-associated cells called mesenchymal stem/stromal cells (MSCs), which we and others have shown promoted TNBC progression by activating pro-malignant signaling in neighboring cancer cells. Characterization of these cascades will better our understanding of TNBC biology, and stands to bring about therapeutics that can eliminate the morbidity and mortality associated with advanced disease. Here, we focused on the emerging class of non-coding RNAs called long non-coding RNAs or lncRNAs, and utilized a MSC-supported TNBC progression model to identify specific family members of functional relevance to TNBC pathogenesis. Indeed, although some have been described to play functional roles in TNBC, activities for lncRNAs as mediators of tumor-microenvironment-driven TNBC development remain to be fully explored. We report that MSCs stimulated robust expression of LINC01119 in TNBC cells, which in turn induced suppressor of cytokine signaling 5 (SOCS5), leading to accelerated cancer cell growth and tumorigenesis. We show that LINC01119 and SOCS5 exhibited tight correlation across multiple breast cancer gene sets and that they were highly enriched in TNBC patient cohorts. Importantly, we present evidence that the LINC01119-SOCS5 axis served as a powerful prognostic indicator of adverse outcome in TNBC patients, and we demonstrate that its repression severely impaired cancer cell growth. Altogether, our findings identify LINC01119 as a major driver of TNBC development, and delineate critical non-coding RNA theranostics of potential translational utility in the management of advanced TNBC, a class of tumors in most need of effective and targeted therapy.
DOI: 10.6084/m9.figshare.14326775
title: Supplementary Fig 5 source data.xlsxCritical roles for the LINC01119-SOCS5 axis as a novel theranostic in triple-negative breast cancersThe LINC01119-SOCS5 axis as a critical theranostic in triple-negative breast cancer

description: The development of triple-negative breast cancer (TNBC) is critically regulated by certain tumor-microenvironment-associated cells called mesenchymal stem/stromal cells (MSCs), which we and others have shown promoted TNBC progression by activating pro-malignant signaling in neighboring cancer cells. Characterization of these cascades will better our understanding of TNBC biology, and stands to bring about therapeutics that can eliminate the morbidity and mortality associated with advanced disease. Here, we focused on the emerging class of non-coding RNAs called long non-coding RNAs or lncRNAs, and utilized a MSC-supported TNBC progression model to identify specific family members of functional relevance to TNBC pathogenesis. Indeed, although some have been described to play functional roles in TNBC, activities for lncRNAs as mediators of tumor-microenvironment-driven TNBC development remain to be fully explored. We report that MSCs stimulated robust expression of LINC01119 in TNBC cells, which in turn induced suppressor of cytokine signaling 5 (SOCS5), leading to accelerated cancer cell growth and tumorigenesis. We show that LINC01119 and SOCS5 exhibited tight correlation across multiple breast cancer gene sets and that they were highly enriched in TNBC patient cohorts. Importantly, we present evidence that the LINC01119-SOCS5 axis served as a powerful prognostic indicator of adverse outcome in TNBC patients, and we demonstrate that its repression severely impaired cancer cell growth. Altogether, our findings identify LINC01119 as a major driver of TNBC development, and delineate critical non-coding RNA theranostics of potential translational utility in the management of advanced TNBC, a class of tumors in most need of effective and targeted therapy.
DOI: 10.6084/m9.figshare.14326781.v1
title: Supplementary Fig 7 source data.xlsxCritical roles for the LINC01119-SOCS5 axis as a novel theranostic in triple-negative breast cancersThe LINC01119-SOCS5 axis as a critical theranostic in triple-negative breast cancer

description: The development of triple-negative breast cancer (TNBC) is critically regulated by certain tumor-microenvironment-associated cells called mesenchymal stem/stromal cells (MSCs), which we and others have shown promoted TNBC progression by activating pro-malignant signaling in neighboring cancer cells. Characterization of these cascades will better our understanding of TNBC biology, and stands to bring about therapeutics that can eliminate the morbidity and mortality associated with advanced disease. Here, we focused on the emerging class of non-coding RNAs called long non-coding RNAs or lncRNAs, and utilized a MSC-supported TNBC progression model to identify specific family members of functional relevance to TNBC pathogenesis. Indeed, although some have been described to play functional roles in TNBC, activities for lncRNAs as mediators of tumor-microenvironment-driven TNBC development remain to be fully explored. We report that MSCs stimulated robust expression of LINC01119 in TNBC cells, which in turn induced suppressor of cytokine signaling 5 (SOCS5), leading to accelerated cancer cell growth and tumorigenesis. We show that LINC01119 and SOCS5 exhibited tight correlation across multiple breast cancer gene sets and that they were highly enriched in TNBC patient cohorts. Importantly, we present evidence that the LINC01119-SOCS5 axis served as a powerful prognostic indicator of adverse outcome in TNBC patients, and we demonstrate that its repression severely impaired cancer cell growth. Altogether, our findings identify LINC01119 as a major driver of TNBC development, and delineate critical non-coding RNA theranostics of potential translational utility in the management of advanced TNBC, a class of tumors in most need of effective and targeted therapy.
DOI: 10.6084/m9.figshare.14326781
title: Supplementary Fig 7 source data.xlsxCritical roles for the LINC01119-SOCS5 axis as a novel theranostic in triple-negative breast cancersThe LINC01119-SOCS5 axis as a critical theranostic in triple-negative breast cancer

description: The development of triple-negative breast cancer (TNBC) is critically regulated by certain tumor-microenvironment-associated cells called mesenchymal stem/stromal cells (MSCs), which we and others have shown promoted TNBC progression by activating pro-malignant signaling in neighboring cancer cells. Characterization of these cascades will better our understanding of TNBC biology, and stands to bring about therapeutics that can eliminate the morbidity and mortality associated with advanced disease. Here, we focused on the emerging class of non-coding RNAs called long non-coding RNAs or lncRNAs, and utilized a MSC-supported TNBC progression model to identify specific family members of functional relevance to TNBC pathogenesis. Indeed, although some have been described to play functional roles in TNBC, activities for lncRNAs as mediators of tumor-microenvironment-driven TNBC development remain to be fully explored. We report that MSCs stimulated robust expression of LINC01119 in TNBC cells, which in turn induced suppressor of cytokine signaling 5 (SOCS5), leading to accelerated cancer cell growth and tumorigenesis. We show that LINC01119 and SOCS5 exhibited tight correlation across multiple breast cancer gene sets and that they were highly enriched in TNBC patient cohorts. Importantly, we present evidence that the LINC01119-SOCS5 axis served as a powerful prognostic indicator of adverse outcome in TNBC patients, and we demonstrate that its repression severely impaired cancer cell growth. Altogether, our findings identify LINC01119 as a major driver of TNBC development, and delineate critical non-coding RNA theranostics of potential translational utility in the management of advanced TNBC, a class of tumors in most need of effective and targeted therapy.
DOI: 10.6084/m9.figshare.14326998
title: Rumen parameters and intake in goats fed cassava chips and alfalfa

description: ABSTRACT Pasture-based production systems and alternative foods can be used to reduce goat milk production costs by around 20%.The objective of this study was to examine the effect of substituting the corn and soybean meal of the concentrate with cassava chips and alfalfa, respectively, on the feed intake, fermentation, and rumen degradability parameters of adult, dry, empty, rumen-cannulated Saanen goats reared in a feedlot. The experiment was conducted as a Latin square design in which the following diets were tested: ground corn and soybean meal; cassava chips and soybean meal; ground corn and alfalfa; and cassava chips and alfalfa. The following variables were evaluated: feed intake, rumen parameters, short-chain fatty acid (SCFA) production, and feed degradation kinetics. Inclusion of cassava chips and alfalfa did not influence feed intake or ammonia production. Rumen pH and SCFA production were influenced by the collection times, presenting a high pH (6.29) at the first measurement (07 h 00) and then decreasing, thus influencing SCFA production throughout the day.Acetic acid and total SCFA production were influenced by the diets, with the highest values for the cassava/alfalfa diet (68.03 and 93.64 mM 100 mM-1, respectively) and the lowest values for the corn/soybean diet (55.40 and 76.03 mM 100 mM-1, respectively).Nutrient digestibility was also influenced by diets, with those containing cassava and cassava/alfalfa, providing the greatest effective degradability (57.57 to 0.02 h-1 and 53.49 to 0.05 h-1; 57.25 to 0.02 h-1and 53.26 to 0.05 h-1, respectively). This finding demonstrates that cassava and alfalfa can substitute the corn and soybean meal of concentrates without changing feed intake or the ruminal environment of goats. Rather, this substitution improves acetic acid production and diet digestibility.
DOI: 10.6084/m9.figshare.14326996
title: Photosynthetic pigments and gas exchange in red and green cabbage under no-tillage and conventional systems

description: ABSTRACT The aim of the present study was to verify changes in the levels of photosynthetic pigments as well as gas exchange in the Fuyutoyo and Red Cabbage cultivars, grown under no-tillage (NT) and conventional (CT) systems. A randomised block design with four replications was used, in a 2 x 2 factorial scheme, where the Fuyutoyo and Red Cabbage cultivars were evaluated under two tillage systems. The levels of chlorophyll a, chlorophyll b, total chlorophyll and anthocyanins in the leaves were evaluated in triplicate. Localised gas exchange was measured at different times of the day, together with different photosynthetic photon flux density curves (PPFD). The Fuyutoyo cultivar showed a higher mean value for chlorophyll a (34.58%), chlorophyll b (35.09%) and total chlorophyll (34.76%), however, no difference was seen between cultivars for the levels of anthocyanin. Greater mean values for chlorophyll a (76.07%), chlorophyll b (77.87%), total chlorophyll (76.74%) and anthocyanin (72.24%) were found under CT compared to the cultivars grown under NT. The relative chlorophyll content of the Red Cabbage plants was influenced by adoption of the no-till system, showing a higher value for the SPAD index. The Fuyutoyo cabbage cultivar showed greater photosynthetic capacity during the day, promoting greater instantaneous carboxylation efficiency. All the cultivars and tillage systems gave a linear reduction in water use efficiency during this period. Under the conventional system, despite presenting higher rates of stomatal conductance, the Red Cabbage cultivar achieved lower values for net photosynthesis when submitted to light-curve analysis.
DOI: 10.6084/m9.figshare.14326996.v1
title: Photosynthetic pigments and gas exchange in red and green cabbage under no-tillage and conventional systems

description: ABSTRACT The aim of the present study was to verify changes in the levels of photosynthetic pigments as well as gas exchange in the Fuyutoyo and Red Cabbage cultivars, grown under no-tillage (NT) and conventional (CT) systems. A randomised block design with four replications was used, in a 2 x 2 factorial scheme, where the Fuyutoyo and Red Cabbage cultivars were evaluated under two tillage systems. The levels of chlorophyll a, chlorophyll b, total chlorophyll and anthocyanins in the leaves were evaluated in triplicate. Localised gas exchange was measured at different times of the day, together with different photosynthetic photon flux density curves (PPFD). The Fuyutoyo cultivar showed a higher mean value for chlorophyll a (34.58%), chlorophyll b (35.09%) and total chlorophyll (34.76%), however, no difference was seen between cultivars for the levels of anthocyanin. Greater mean values for chlorophyll a (76.07%), chlorophyll b (77.87%), total chlorophyll (76.74%) and anthocyanin (72.24%) were found under CT compared to the cultivars grown under NT. The relative chlorophyll content of the Red Cabbage plants was influenced by adoption of the no-till system, showing a higher value for the SPAD index. The Fuyutoyo cabbage cultivar showed greater photosynthetic capacity during the day, promoting greater instantaneous carboxylation efficiency. All the cultivars and tillage systems gave a linear reduction in water use efficiency during this period. Under the conventional system, despite presenting higher rates of stomatal conductance, the Red Cabbage cultivar achieved lower values for net photosynthesis when submitted to light-curve analysis.
DOI: 10.6084/m9.figshare.14326999.v1
title: Semen cryopreservation in Piaractus mesopotamicus: the effect of different diluents and freezing equipment

description: ABSTRACT Piaractus mesopotamicus (Holmberg, 1887) is a fish native to the Basin of the River Plate and which has great economic importance. In view of this, there has been interest in developing biotechnologies that aim to optimise reproduction in captivity. The aim of this study was to verify the effect of freezing the semen of the pacu using different diluents and freezing equipment. The semen, after pooling, was diluted in the proportion 1:3 (semen: diluent) in two different freezing media, resulting from combining 10% DMSO with Glucose 5% or with ACP-104; it was then cryopreserved in a Dry Shipper or programmable freezer (PF). The samples were later thawed and evaluated for sperm kinetics, membrane integrity and sperm morphology using the Computer Assisted Sperm Analyser (CASA). The data were submitted to ANOVA, and the mean values compared by F-test (P &lt;0.05). There was no interaction between the Glucose 5% or ACP-104 diluents, and the Dry Shipper or PF equipment. However, Glucose 5% showed better results for sperm kinetics (motility: 40.52 ± 7.1%; VCL: 28.53 ± 4.07 µm s-1; VSL: 9.38 ± 3.54 µm s-1 and VAP: 15.24 ± 4.23 µm s-1) compared to ACP-104 (motility: 31.19 ± 3.17%; VCL: 22.76 ± 1.86 µm s-1; VSL: 5.4 ± 1.16 µm s-1 and VAP: 10.06 ± 1.53 µm s-1). It can therefore be concluded that Glucose 5% gives the best results compared to ACP-104, regardless of the equipment used, Dry Shipper or PF, in freezing the semen of P. mesopotamicus.
DOI: 10.6084/m9.figshare.14326998.v1
title: Rumen parameters and intake in goats fed cassava chips and alfalfa

description: ABSTRACT Pasture-based production systems and alternative foods can be used to reduce goat milk production costs by around 20%.The objective of this study was to examine the effect of substituting the corn and soybean meal of the concentrate with cassava chips and alfalfa, respectively, on the feed intake, fermentation, and rumen degradability parameters of adult, dry, empty, rumen-cannulated Saanen goats reared in a feedlot. The experiment was conducted as a Latin square design in which the following diets were tested: ground corn and soybean meal; cassava chips and soybean meal; ground corn and alfalfa; and cassava chips and alfalfa. The following variables were evaluated: feed intake, rumen parameters, short-chain fatty acid (SCFA) production, and feed degradation kinetics. Inclusion of cassava chips and alfalfa did not influence feed intake or ammonia production. Rumen pH and SCFA production were influenced by the collection times, presenting a high pH (6.29) at the first measurement (07 h 00) and then decreasing, thus influencing SCFA production throughout the day.Acetic acid and total SCFA production were influenced by the diets, with the highest values for the cassava/alfalfa diet (68.03 and 93.64 mM 100 mM-1, respectively) and the lowest values for the corn/soybean diet (55.40 and 76.03 mM 100 mM-1, respectively).Nutrient digestibility was also influenced by diets, with those containing cassava and cassava/alfalfa, providing the greatest effective degradability (57.57 to 0.02 h-1 and 53.49 to 0.05 h-1; 57.25 to 0.02 h-1and 53.26 to 0.05 h-1, respectively). This finding demonstrates that cassava and alfalfa can substitute the corn and soybean meal of concentrates without changing feed intake or the ruminal environment of goats. Rather, this substitution improves acetic acid production and diet digestibility.
DOI: 10.6084/m9.figshare.14326999
title: Semen cryopreservation in Piaractus mesopotamicus: the effect of different diluents and freezing equipment

description: ABSTRACT Piaractus mesopotamicus (Holmberg, 1887) is a fish native to the Basin of the River Plate and which has great economic importance. In view of this, there has been interest in developing biotechnologies that aim to optimise reproduction in captivity. The aim of this study was to verify the effect of freezing the semen of the pacu using different diluents and freezing equipment. The semen, after pooling, was diluted in the proportion 1:3 (semen: diluent) in two different freezing media, resulting from combining 10% DMSO with Glucose 5% or with ACP-104; it was then cryopreserved in a Dry Shipper or programmable freezer (PF). The samples were later thawed and evaluated for sperm kinetics, membrane integrity and sperm morphology using the Computer Assisted Sperm Analyser (CASA). The data were submitted to ANOVA, and the mean values compared by F-test (P &lt;0.05). There was no interaction between the Glucose 5% or ACP-104 diluents, and the Dry Shipper or PF equipment. However, Glucose 5% showed better results for sperm kinetics (motility: 40.52 ± 7.1%; VCL: 28.53 ± 4.07 µm s-1; VSL: 9.38 ± 3.54 µm s-1 and VAP: 15.24 ± 4.23 µm s-1) compared to ACP-104 (motility: 31.19 ± 3.17%; VCL: 22.76 ± 1.86 µm s-1; VSL: 5.4 ± 1.16 µm s-1 and VAP: 10.06 ± 1.53 µm s-1). It can therefore be concluded that Glucose 5% gives the best results compared to ACP-104, regardless of the equipment used, Dry Shipper or PF, in freezing the semen of P. mesopotamicus.
DOI: 10.6084/m9.figshare.14327001
title: Agronomic and economic feasibility indicators for cowpea-vegetable under green manure in a semiarid environment

description: ABSTRACT Two experiments were conducted in different areas of the Rafael Fernandes Experimental Farm, located in Lagoinha district, 20 km from Mossoró municipality (RN), in the period from June to September of the years of 2015 and 2016 with the objective of evaluating and estimating agronomic and economic feasibility indicators of cowpea-vegetable as a function of amounts of hairy woodrose (Merremia aegyptia (L.) Urb.) biomass incorporated into the soil in two crops. Treatments consisted of the following amounts of hairy woodrose biomass: 20, 35, 50 and 65 t ha-1 on a dry basis. The cultivar of cowpea-vegetable planted was “BRS Itaim”. The evaluated characteristics were: length of green pods, number of green pods per m2, productivity and dry mass of green pods, weight of 100 green grains, yield and dry mass of green grains. In addition, the following economic indicators were evaluated: gross income, net income, rate of return and profitability index. The optimization of the agronomic efficiency of the cowpea-vegetable was obtained in the amount of 45 t ha-1 of hairy woodrose biomass incorporated into the soil and the optimization of the agroeconomic viability of the cowpea-vegetable cultivation was reached in the quantity of hairy woodrose biomass of 37.70 t ha-1 added to the ground. The first growing crop yielded better agroeconomic efficiency of cowpea-vegetable than the second crop. The use of hairy woodrose as green manure is economically feasible and recommended to the farmer in the production of cowpea-vegetable under the evaluated conditions.
DOI: 10.6084/m9.figshare.14327004
title: Study on the growth curve of the internal cavity of ‘Dwarf green’ coconut fruits

description: ABSTRACT The aim of the work was to evaluate the adjustment of the logistic and gompertz model with structure of first-order autoregressive errors in the study on the ‘Dwarf green’ coconut fruit growth based on longitudinal and cross-sectional internal cavity diameter data (DLCI and DTCI). Model adjustments showed positive residual autocorrelation, according to the Durbin-Watson test and for both variables, DLCI and DTCI, the residue was modeled according to first-order autoregressive process (AR1). The analysis was performed using the least squares method in the PROC MODEL of the SAS software and results indicated that for both characteristics under study, the logistic model was the most appropriate in describing fruit growth and, according to the model, fully developed ‘Dwarf green’ coconut fruits have longitudinal and cross-sections internal cavity diameter of approximately 7.39 cm and 7.60 cm, respectively.
DOI: 10.6084/m9.figshare.14327004.v1
title: Study on the growth curve of the internal cavity of ‘Dwarf green’ coconut fruits

description: ABSTRACT The aim of the work was to evaluate the adjustment of the logistic and gompertz model with structure of first-order autoregressive errors in the study on the ‘Dwarf green’ coconut fruit growth based on longitudinal and cross-sectional internal cavity diameter data (DLCI and DTCI). Model adjustments showed positive residual autocorrelation, according to the Durbin-Watson test and for both variables, DLCI and DTCI, the residue was modeled according to first-order autoregressive process (AR1). The analysis was performed using the least squares method in the PROC MODEL of the SAS software and results indicated that for both characteristics under study, the logistic model was the most appropriate in describing fruit growth and, according to the model, fully developed ‘Dwarf green’ coconut fruits have longitudinal and cross-sections internal cavity diameter of approximately 7.39 cm and 7.60 cm, respectively.