DOI: 10.5281/zenodo.1133329
title: Seven Fluorescent Proteins Profile In Chlamydomonas Reinhardtii And R Code Statistic For Analysis

description: <strong>Overview</strong>

Data points present in this dataset were obtained following the protocol described in dx.doi.org/10.17504/protocols.io.kfnctme.  We picked transformed colonies and cultured in 400 μL TAP medium for 7 days in Deep-well plates (Corning Axygen®, No.: PDW500CS, Thermo Fisher Scientific Inc., Waltham, MA), covered with Breathe-Easy® (Sigma-Aldrich®). Cultivation was performed on a rotary shaker, set to 150 rpm, under constant illumination (50 μmol photons/m<sup>2</sup>s). Then 100 μL sample were transferred clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA) and fluorescence was measured using an Infinite® M200 PRO plate reader (Tecan, Männedorf, Switzerland). Supernatant samples were obtained by spinning Deep-well plates at 3000 × <em>g</em> for 10 min and transferring 100 μL from each well to the clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA), followed by fluorescence measurement. To compare the constructs, R Statistic version 3.3.3 was used to perform one-way ANOVA (with Tukey's test), and to test statistical hypotheses, the significance level was set at 0.05. Graphs were generated in RStudio v1.0.136. The codes are deposit herein.

<strong>Info</strong>

ANOVA_Turkey_Sub.R           -&gt; code for ANOVA analysis in R statistic 3.3.3

Anova_Output_Summary_Guide.pdf -&gt; Explain the ANOVA files content

Analysis_Raw_FP.xlsx  -&gt; File with raw values organized in a spreadsheet 

pRFU_<strong>FLUORESCENT PROTEIN</strong>_+_bk.csv                     -&gt; relative supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii </em>

sup_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.csv                         -&gt;  supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> 

who_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.csv                       -&gt; whole culture mCherry  fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> 

pRFU_<strong>FLUORESCENT PROTEIN</strong>_+_bk.doc      -&gt; ANOVA of relative supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> 

sup_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.doc          -&gt; ANOVA of supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> 

who_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.doc          -&gt; ANOVA of whole culture mCherry  fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> 

 

<strong>Consider citing our work. </strong>

1. Molino JVD, de Carvalho JCM, Mayfield S. Evaluation of secretion reporters to microalgae biotechnology: blue to red fluorescent proteins. Algal Res. 2018;31: 252–261. doi:10.1016/j.algal.2018.02.018