cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8292518.v1
title: Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected Colossoma macropomum (Cuvier, 1818) exposed to hypoxia

description: Abstract The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2
DOI: 10.6084/m9.figshare.8292509
title: Mineral content, phenolic compounds and bioactive amines of cheese bread enriched with cowpea

description: ABSTRACT Cheese bread presents great acceptance and market expansion. Due to the lack of standardization in its manufacturing, various ingredients can be added. It was aimed to determine the content of minerals, bioactive compounds and antioxidant activity in cheese bread enriched with biofortified cowpea. Two formulations were prepared, standard cheese bread (without cowpea) and enriched cheese bread. The minerals were analyzed using the plasma emission spectrometry technique with inductive coupling, phenolic compounds and antioxidant activity were determined by spectrophotometry and the amines by High Performance Liquid Chromatography. All analyzes were performed in triplicate and for the comparison of the means between two variables, Student's t-test was applied and for multiple comparisons, the Tukey test. It was observed that the enriched cheese bread is source of copper, zinc and sodium and presents high content of calcium, phosphorus and magnesium, considering the recommendations for the children's audience. The concentrations of total phenolics, flavonoids and condensed tannins increased significantly with the introduction of cowpea. It was also observed an significant increase of spermidine, cadaverine and agmatine. The antioxidant activity was statistically higher in enriched cheese bread. It is concluded, therefore, that cheese bread with cowpea contributes to the supply of minerals and bioactive compounds.
DOI: 10.6084/m9.figshare.8292500
title: Wastewater infrastructure and the ecology and management of freshwater systems

description: Abstract Globally, freshwater resources are influenced by inputs of energy, nutrients, and pollutants from human wastewater. Local resource managers and policy-makers are tasked to address ecological and human-health concerns associated with aging and obsolete water infrastructure using limited financial resources. Nevertheless, there is limited information available describing how waste streams vary in their pollutant load or their subsequent effects on ecosystem structure and function in streams and rivers. Consequently, as wastewater systems degrade, local resource managers and policy makers are forced to develop watershed management strategies to deal with increasing effluent discharge without an understanding of how their decisions will influence local ecological processes or the structural and functional integrity of downstream habitats. Here, I discuss some of the ecological implications of obsolete or absent wastewater treatment, and describe how mismatches between the governance of wastewater management and watershed ecology may exacerbate environmental problems.
DOI: 10.6084/m9.figshare.8292503
title: Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi

description: Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps.
DOI: 10.6084/m9.figshare.8292509.v1
title: Mineral content, phenolic compounds and bioactive amines of cheese bread enriched with cowpea

description: ABSTRACT Cheese bread presents great acceptance and market expansion. Due to the lack of standardization in its manufacturing, various ingredients can be added. It was aimed to determine the content of minerals, bioactive compounds and antioxidant activity in cheese bread enriched with biofortified cowpea. Two formulations were prepared, standard cheese bread (without cowpea) and enriched cheese bread. The minerals were analyzed using the plasma emission spectrometry technique with inductive coupling, phenolic compounds and antioxidant activity were determined by spectrophotometry and the amines by High Performance Liquid Chromatography. All analyzes were performed in triplicate and for the comparison of the means between two variables, Student's t-test was applied and for multiple comparisons, the Tukey test. It was observed that the enriched cheese bread is source of copper, zinc and sodium and presents high content of calcium, phosphorus and magnesium, considering the recommendations for the children's audience. The concentrations of total phenolics, flavonoids and condensed tannins increased significantly with the introduction of cowpea. It was also observed an significant increase of spermidine, cadaverine and agmatine. The antioxidant activity was statistically higher in enriched cheese bread. It is concluded, therefore, that cheese bread with cowpea contributes to the supply of minerals and bioactive compounds.
DOI: 10.6084/m9.figshare.8292500.v1
title: Wastewater infrastructure and the ecology and management of freshwater systems

description: Abstract Globally, freshwater resources are influenced by inputs of energy, nutrients, and pollutants from human wastewater. Local resource managers and policy-makers are tasked to address ecological and human-health concerns associated with aging and obsolete water infrastructure using limited financial resources. Nevertheless, there is limited information available describing how waste streams vary in their pollutant load or their subsequent effects on ecosystem structure and function in streams and rivers. Consequently, as wastewater systems degrade, local resource managers and policy makers are forced to develop watershed management strategies to deal with increasing effluent discharge without an understanding of how their decisions will influence local ecological processes or the structural and functional integrity of downstream habitats. Here, I discuss some of the ecological implications of obsolete or absent wastewater treatment, and describe how mismatches between the governance of wastewater management and watershed ecology may exacerbate environmental problems.
DOI: 10.6084/m9.figshare.8292503.v1
title: Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi

description: Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps.
DOI: 10.6084/m9.figshare.8292491
title: Visual symptoms and nutritional deficiencies in olive plants subjected to nutrient deprivation

description: ABSTRACT. As the fourth largest importer of olive oil and the fifth largest importer of olives, Brazil is one of the largest importers of olive (Olea europaea L.) products in the world. In recent decades, the introduction and growth of olive cultivars with lower chilling requirements in the south and southeast regions of Brazil have made olive production viable in the country. However, there is a dearth of information about the management of olive crops in Brazil, especially in relation to studies about the nutritional needs of olive trees grown in subtropical regions, which may enable advances in the productivity of this fruit. The aim of this study was to evaluate the growth, dry matter production and nutritional status of the olive tree under the effect of nutrient omissions, as well as to establish visual diagnostic parameters of nutrient deficiencies. We used a completely randomized design with ten treatments and three replicates grown in the Hoagland-Arnon nutrient solution and solutions with individual omissions of N, P, K, Ca, Mg, S, B, Fe, and Zn. The treatments that most limited the growth of olive trees were the N, Ca, and B omission treatments.
DOI: 10.6084/m9.figshare.8292497
title: Intercropping Between Corn and Urochloa brizantha Managed with Mesotrione Underdoses

description: ABSTRACT: Corn and forage intercropping system has been commonly used for pasture establishment or renewal. In addition, the use of reduced doses of herbicides selective to corn has facilitated coexistence by reducing competitiveness against forage species. This study aimed to evaluate the effects of mesotrione underdoses in a corn and Urochloa brizantha intercropping on weed population dynamics, corn yield, and forage performance and nutritional quality. For this purpose, five mesotrione doses were tested (0.0, 9.6, 19.2, 38.4, and 57.6 g ha-1) in corn and forage intercropping and monocultures. Individual density and weed dry matter were influenced by mesotrione doses, intercropping, and precipitation distribution over the evaluated period in the intercropping and pasture. In intercropping, Alternanthera tenella and Commelina benghalensis were the most important species because they could not be controlled by the herbicide. After intercropping, A. tenella and C. benghalensis presented higher relative importance (RI) in the formed pasture during the rainy season, while Conyza bonariensis and A. tenella presented the highest RI during the dry season. Corn grain yield was not affected by the presence of grass, but forage yield was affected by the competition with corn. Mesotrione doses did not affect the yield and nutritional quality of the forage. The effects on mineral matter, crude protein, and acid detergent fiber in the forage were due to the coexistence with corn when compared to the control grown in monoculture.
DOI: 10.6084/m9.figshare.8292485
title: Allelopathic Potential of Ethanolic Extract and Phytochemical Analysis of Paspalum maritimum Trind

description: ABSTRACT: Allelopathy is defined as the ability of certain plants and microorganisms to interfere with the metabolism of other species through substances released into the environment, being an alternative to control weeds and diseases. In this context, this study aimed to evaluate the allelopathic potential and identify groups of secondary metabolites of Paspalum maritimum Trind. The species Lactuca sativa, Digitaria insularis, Emilia coccinea, and Portulaca oleracea were used as recipient plants. The ethanolic extract was obtained from shoot and root of donor species to evaluate the allelopathic potential. Potentially allelopathic effects were evaluated by means of germination tests, germination speed index, and initial seedling growth. A phytochemical analysis of the extract was also performed to identify the secondary metabolites. The ethanolic extract from both plant parts had an allelopathic effect on recipient species. The presence of condensed tannins, chalcones and aurones, flavonones, steroids, and saponins were detected in the most active fraction of the shoot of P. maritimum.
DOI: 10.6084/m9.figshare.8292488
title: Bioassay for Determining Persistence of the Herbicide Saflufenacil in an Oxisol

description: ABSTRACT: Persistence of an herbicide is defined as the ability to maintain the integrity of its molecule and chemical, physical, and phytotoxic properties in the environment where it was applied. Knowledge of persistence has implications both for the toxicity generated on species grown in succession and for the period in which the herbicide controls weeds. This study aimed to assess the persistence of the herbicide saflufenacil in an Oxisol by means of bioassays. Two experiments with the application of 29.4 g ha-1 of saflufenacil were carried out under field conditions: the first in the 2011/2012 season and the second in the 2012/2013 season. Soil samples were collected at depths of up to 10 cm at different times after application. Subsequently, two bioassays were carried out in a greenhouse by using beet (Beta vulgaris L.) and cucumber (Cucumis sativus L.) as bioindicators. The studies were carried out in a completely randomized design with four replications. Treatments consisted of periods after saflufenacil application (0, 5, 10, 15, 25, 35, 50, and 100 days). The crop was also considered as a factor. Stand, height, and phytotoxicity of plants were assessed in both agricultural seasons at 14, 21, and 28 days after sowing (DAS), as well as shoot fresh (SFM) and dry matter (SDM) at 28 DAS. In general, saflufenacil persistence was between 25 and 35 days. Beet was more sensitive to the presence of saflufenacil in the soil when compared to cucumber. Herbicide effects were higher in the 2012/2013 season when compared to the 2011/2012 season.
DOI: 10.6084/m9.figshare.8292488.v1
title: Bioassay for Determining Persistence of the Herbicide Saflufenacil in an Oxisol

description: ABSTRACT: Persistence of an herbicide is defined as the ability to maintain the integrity of its molecule and chemical, physical, and phytotoxic properties in the environment where it was applied. Knowledge of persistence has implications both for the toxicity generated on species grown in succession and for the period in which the herbicide controls weeds. This study aimed to assess the persistence of the herbicide saflufenacil in an Oxisol by means of bioassays. Two experiments with the application of 29.4 g ha-1 of saflufenacil were carried out under field conditions: the first in the 2011/2012 season and the second in the 2012/2013 season. Soil samples were collected at depths of up to 10 cm at different times after application. Subsequently, two bioassays were carried out in a greenhouse by using beet (Beta vulgaris L.) and cucumber (Cucumis sativus L.) as bioindicators. The studies were carried out in a completely randomized design with four replications. Treatments consisted of periods after saflufenacil application (0, 5, 10, 15, 25, 35, 50, and 100 days). The crop was also considered as a factor. Stand, height, and phytotoxicity of plants were assessed in both agricultural seasons at 14, 21, and 28 days after sowing (DAS), as well as shoot fresh (SFM) and dry matter (SDM) at 28 DAS. In general, saflufenacil persistence was between 25 and 35 days. Beet was more sensitive to the presence of saflufenacil in the soil when compared to cucumber. Herbicide effects were higher in the 2012/2013 season when compared to the 2011/2012 season.
DOI: 10.6084/m9.figshare.8292491.v1
title: Visual symptoms and nutritional deficiencies in olive plants subjected to nutrient deprivation

description: ABSTRACT. As the fourth largest importer of olive oil and the fifth largest importer of olives, Brazil is one of the largest importers of olive (Olea europaea L.) products in the world. In recent decades, the introduction and growth of olive cultivars with lower chilling requirements in the south and southeast regions of Brazil have made olive production viable in the country. However, there is a dearth of information about the management of olive crops in Brazil, especially in relation to studies about the nutritional needs of olive trees grown in subtropical regions, which may enable advances in the productivity of this fruit. The aim of this study was to evaluate the growth, dry matter production and nutritional status of the olive tree under the effect of nutrient omissions, as well as to establish visual diagnostic parameters of nutrient deficiencies. We used a completely randomized design with ten treatments and three replicates grown in the Hoagland-Arnon nutrient solution and solutions with individual omissions of N, P, K, Ca, Mg, S, B, Fe, and Zn. The treatments that most limited the growth of olive trees were the N, Ca, and B omission treatments.
DOI: 10.6084/m9.figshare.8292497.v1
title: Intercropping Between Corn and Urochloa brizantha Managed with Mesotrione Underdoses

description: ABSTRACT: Corn and forage intercropping system has been commonly used for pasture establishment or renewal. In addition, the use of reduced doses of herbicides selective to corn has facilitated coexistence by reducing competitiveness against forage species. This study aimed to evaluate the effects of mesotrione underdoses in a corn and Urochloa brizantha intercropping on weed population dynamics, corn yield, and forage performance and nutritional quality. For this purpose, five mesotrione doses were tested (0.0, 9.6, 19.2, 38.4, and 57.6 g ha-1) in corn and forage intercropping and monocultures. Individual density and weed dry matter were influenced by mesotrione doses, intercropping, and precipitation distribution over the evaluated period in the intercropping and pasture. In intercropping, Alternanthera tenella and Commelina benghalensis were the most important species because they could not be controlled by the herbicide. After intercropping, A. tenella and C. benghalensis presented higher relative importance (RI) in the formed pasture during the rainy season, while Conyza bonariensis and A. tenella presented the highest RI during the dry season. Corn grain yield was not affected by the presence of grass, but forage yield was affected by the competition with corn. Mesotrione doses did not affect the yield and nutritional quality of the forage. The effects on mineral matter, crude protein, and acid detergent fiber in the forage were due to the coexistence with corn when compared to the control grown in monoculture.
DOI: 10.6084/m9.figshare.8292479
title: Use of water treatment sludge (WTS) as fine aggregate in concretes: evaluation of physical-mechanical properties

description: ABSTRACT The present work has an objective to investigate the use of water treatment plant sludge (WTS) as fine aggre-gate in concretes. Were evaluated the effects of different sand replacement levels (2.5%, 5%, 7.5% and 10% by mass) by WTS and the w/c ratio (0.4, 0.5 and 0.6) in physical (bulk density, porosity and water absorption by immersion) and mechanical properties (compressive and splitting tensile strength) at different ages (7, 28 and 91 days). All the collected data were statistically analyzed using analysis of variance (ANOVA). The results showed that, despite the minimization of the properties in comparison with the reference concrete, all concretes showed values of compressive strength greater than 25 MPa at 91 days considering the highest w/c ratio (0.60). Therefore, the WTS can be used in concretes, considering the properties analyzed. In this way, the present study shows that the WTS can be used as fine aggregate in concrete, being a valid and sustainable alternative for use in construction.
DOI: 10.6084/m9.figshare.8292485.v1
title: Allelopathic Potential of Ethanolic Extract and Phytochemical Analysis of Paspalum maritimum Trind

description: ABSTRACT: Allelopathy is defined as the ability of certain plants and microorganisms to interfere with the metabolism of other species through substances released into the environment, being an alternative to control weeds and diseases. In this context, this study aimed to evaluate the allelopathic potential and identify groups of secondary metabolites of Paspalum maritimum Trind. The species Lactuca sativa, Digitaria insularis, Emilia coccinea, and Portulaca oleracea were used as recipient plants. The ethanolic extract was obtained from shoot and root of donor species to evaluate the allelopathic potential. Potentially allelopathic effects were evaluated by means of germination tests, germination speed index, and initial seedling growth. A phytochemical analysis of the extract was also performed to identify the secondary metabolites. The ethanolic extract from both plant parts had an allelopathic effect on recipient species. The presence of condensed tannins, chalcones and aurones, flavonones, steroids, and saponins were detected in the most active fraction of the shoot of P. maritimum.
DOI: 10.6084/m9.figshare.8292479.v1
title: Use of water treatment sludge (WTS) as fine aggregate in concretes: evaluation of physical-mechanical properties

description: ABSTRACT The present work has an objective to investigate the use of water treatment plant sludge (WTS) as fine aggre-gate in concretes. Were evaluated the effects of different sand replacement levels (2.5%, 5%, 7.5% and 10% by mass) by WTS and the w/c ratio (0.4, 0.5 and 0.6) in physical (bulk density, porosity and water absorption by immersion) and mechanical properties (compressive and splitting tensile strength) at different ages (7, 28 and 91 days). All the collected data were statistically analyzed using analysis of variance (ANOVA). The results showed that, despite the minimization of the properties in comparison with the reference concrete, all concretes showed values of compressive strength greater than 25 MPa at 91 days considering the highest w/c ratio (0.60). Therefore, the WTS can be used in concretes, considering the properties analyzed. In this way, the present study shows that the WTS can be used as fine aggregate in concrete, being a valid and sustainable alternative for use in construction.