cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14061215
title: Estimating epidemiological and economic burden and community derived disability weights for snakebite in Kerala: A study protocol

description: The documents relate to informed consent, questionnaire, and VAS tool for administration for the epidemiological and economic burden study of snakebites in Kerala state.
DOI: 10.6084/m9.figshare.14061215.v2
title: Estimating epidemiological and economic burden and community derived disability weights for snakebite in Kerala: A study protocol

description: The documents relate to informed consent, questionnaire, and VAS tool for administration for the epidemiological and economic burden study of snakebites in Kerala state.
DOI: 10.25919/xmra-nj40
title: Parkes observations for project P1050 semester 2021APRS_04

description: This is a request for observing time for the timing campaign of new pulsar discoveries from the reprocessing of the low-latitude Galactic plane section of the HTRU survey (P630). We have now completed 80 % of the survey with GPU based coherent acceleration search, template bank and fast folding algorithm pipelines, and now have discovered 68 previously unknown pulsars and 21 "class A" pulsars awaiting confirmation. Interesting science can usually only be derived from a new pulsar after confirmation and a follow-up timing campaign is carried out. One year of initial timing is the minimum timespan required to fully characterise any newly-discovered pulsars, essential for deriving pulsar parameters such as the characteristic age, magnetic field strength, spin-down rate, as well as to detect any unexpected behaviour of the pulsar which might result from emission instabilities. This timing project is necessary for the timing of the new double neutron star (DNS) system J1325-6253 discovered last semester and for following up our confirmed/to-be confirmed new pulsars from the HTRU Galactic plane survey. Pulsars on the observing list here have been confirmed and remaining candidates will be confirmed in the current 2020OCTS semester, the follow-up timing in 2021APRS will produce completely new and exciting results. In addition, this timing project will allow us to measure the advance of periastron of PSR J1325-6253, true Galactic plane pulsar population and boundaries of pulsar parameter space in the galactic plane.
DOI: 10.6084/m9.figshare.13041961.v1
title: <i>Corallina</i> (Corallinales, Rhodophyta) from Tristan da Cunha and the Falkland Islands: implications for South Atlantic biogeography

description: A molecular and morphological taxonomic study of <i>Corallina</i> (Corallinales, Rhodophyta) from Tristan da Cunha and the Falkland Islands revealed <i>Corallina chamberlainiae</i> J.Brodie & R.Mrowicki sp. nov. from both South Atlantic archipelagos, and <i>Corallina</i> cf. <i>caespitosa</i> only in the Falkland Islands. Analysis of mitochondrial COI-5P and plastid <i>psb</i>A resolved <i>C. chamberlainiae</i> as a distinct clade composed of specimens from Tristan and the Falkland Islands (COI-5P), in addition to two matching New Zealand samples (<i>psb</i>A). In <i>psb</i>A analyses, <i>C</i>. cf. <i>caespitosa</i> was close to two species from Japan (as <i>C. pilulifera</i> and <i>C. melobesioides</i>) but was separate from <i>C. caespitosa</i> sensu stricto from Britain and <i>C. ferreyrae</i> (isotype) from Peru. In COI-5P analyses, <i>C</i>. cf. <i>caespitosa</i> was in a clade with <i>C. caespitosa</i> (holotype) and close to, but distinct from, <i>C. ferreyrae</i> and an unidentified South African <i>Corallina</i> species. Application of the Automatic Barcode Gap Discovery (ABGD) and Poisson tree processes (bPTP) for COI-5P lumped <i>C. chamberlainiae</i> into one clade, whereas the Generalized Mixed Yule Coalescent (GMYC) model split it into two well-supported groups, one of which only contained Falkland Island specimens. All three models lumped <i>C</i>. cf. <i>caespitosa</i> with <i>C. caespitosa. C. chamberlainiae</i> closely resembles <i>C. officinalis</i>, the generitype, but is distinguished by smaller size and a more compressed thallus towards the apex. <i>Corallina</i> cf. <i>caespitosa</i> resembles <i>C. caespitosa</i> but is smaller and has fused, palm-like upper fronds. Neither of these taxa is conspecific with <i>C. ferreyrae</i> although <i>C. caespitosa</i> was recently synonymized with <i>C. ferreyrae. C. chamberlainiae</i> and <i>C</i>. cf. <i>caespitosa</i> occupy similar intertidal habitats to their respective, closely related counterparts <i>C. officinalis</i> and <i>C. caespitosa</i>. The results indicate cryptic diversity and suggest that there are many misidentified <i>Corallina</i> species. The paucity of South Atlantic studies of the Corallinales points to the need for a much greater taxonomic effort in this biogeographic region.
DOI: 10.6084/m9.figshare.12853569
title: Morphology and molecular phylogeny of <i>Umbraulva</i> spp. (Ulvales, Ulvophyceae), and proposal of <i>Ryuguphycus</i> gen. nov. and <i>R. kuaweuweu</i> comb. nov.

description: The reproductive structures, early development and carotenoid composition of <i>Umbraulva kuaweuweu</i>, which is a deep-water ulvacean species recently reported from Hawaii, are reported for the first time using the specimens collected by dredging at ~30 m depth from Ogasawara Islands and Mageshima in southern Japan. Although <i>Umbraulva kuaweuweu</i> had siphonaxanthin as in <i>U. japonica</i>, a characteristic carotenoid of <i>Umbraulva, U. kuaweuweu</i> lacked α-carotene and β-carotene. <i>U. kuaweuweu</i> differed markedly from <i>U. japonica</i> in thallus morphology and reproductive structures: cell shape, presence/absence of inner rhizoidal filaments in the upper parts of thalli, solid/partly saccate thalli, and developmental processes of zoids. Multigene concatenated DNA sequences of chloroplast <i>rbc</i>L and <i>tuf</i>A genes, and nuclear 18S, 5.8S, 28S rDNA and rDNA ITS1 and ITS2 regions indicated separation of <i>U. kuaweuweu</i> from the clade comprised of the generitype <i>U. japonica, U. amamiensis, U. dangeardii</i> and <i>U. kaloakulau</i>. In conclusion, based on the distinctive morphology, life history, carotenoid composition and molecular phylogeny, we propose the establishment of a new genus <i>Ryuguphycus</i> gen. nov. to accommodate <i>U. kuaweuweu</i>, as <i>R. kuaweuweu</i> comb. nov.
DOI: 10.6084/m9.figshare.12853566.v1
title: Zinc-induced stress on cells of <i>Halamphora luciae</i> (Bacillariophyceae)

description: The aim of the present paper was to study the effect of zinc on the benthic diatom <i>Halamphora luciae</i>. Bioassays using the metal concentration corresponding to the IC<sub>zn</sub>50 were terminated in two selected endpoints: day 4 (acute phase) and day 9 (chronic phase). A 1.4- and 2.2-fold reduction in Zn<sup>2+</sup> with respect to the initial cation concentration was estimated by atomic absorption at days 4 and 9, respectively and was attributed both to precipitation of insoluble carbonates and oxides at alkaline pH and to biosorption. Carrying capacity of Zn-exposed cultures diminished even when growth rate remained unchanged, suggesting some degree of acclimation of the strain to metal stress. Zn stimulated cell bleaching together with the shift into the lipogenic phase, denoted by total lipid quantification and bigger lipid bodies volumes. Lipid peroxidation and phenolic compounds dosage revealed no major metal-induced oxidative stress. On the other hand, metal exposure had an antagonistic effect on the activation of alkaline phosphatase activity after phosphorus starvation. Superoxide dismutase (SOD) activity, cell motility and mucilage secretion were enhanced only during acute exposure. Soluble extracellular polymeric substances (S-EPS) from Zn-exposed cultures denoted a higher heterogeneity in monosaccharide composition. Frustules from metal-exposed cells presented a reduction in areolae diameter together with a lower area:volume ratio of the cells due to the addition of cingular bands.
DOI: 10.6084/m9.figshare.13041961
title: <i>Corallina</i> (Corallinales, Rhodophyta) from Tristan da Cunha and the Falkland Islands: implications for South Atlantic biogeography

description: A molecular and morphological taxonomic study of <i>Corallina</i> (Corallinales, Rhodophyta) from Tristan da Cunha and the Falkland Islands revealed <i>Corallina chamberlainiae</i> J.Brodie & R.Mrowicki sp. nov. from both South Atlantic archipelagos, and <i>Corallina</i> cf. <i>caespitosa</i> only in the Falkland Islands. Analysis of mitochondrial COI-5P and plastid <i>psb</i>A resolved <i>C. chamberlainiae</i> as a distinct clade composed of specimens from Tristan and the Falkland Islands (COI-5P), in addition to two matching New Zealand samples (<i>psb</i>A). In <i>psb</i>A analyses, <i>C</i>. cf. <i>caespitosa</i> was close to two species from Japan (as <i>C. pilulifera</i> and <i>C. melobesioides</i>) but was separate from <i>C. caespitosa</i> sensu stricto from Britain and <i>C. ferreyrae</i> (isotype) from Peru. In COI-5P analyses, <i>C</i>. cf. <i>caespitosa</i> was in a clade with <i>C. caespitosa</i> (holotype) and close to, but distinct from, <i>C. ferreyrae</i> and an unidentified South African <i>Corallina</i> species. Application of the Automatic Barcode Gap Discovery (ABGD) and Poisson tree processes (bPTP) for COI-5P lumped <i>C. chamberlainiae</i> into one clade, whereas the Generalized Mixed Yule Coalescent (GMYC) model split it into two well-supported groups, one of which only contained Falkland Island specimens. All three models lumped <i>C</i>. cf. <i>caespitosa</i> with <i>C. caespitosa. C. chamberlainiae</i> closely resembles <i>C. officinalis</i>, the generitype, but is distinguished by smaller size and a more compressed thallus towards the apex. <i>Corallina</i> cf. <i>caespitosa</i> resembles <i>C. caespitosa</i> but is smaller and has fused, palm-like upper fronds. Neither of these taxa is conspecific with <i>C. ferreyrae</i> although <i>C. caespitosa</i> was recently synonymized with <i>C. ferreyrae. C. chamberlainiae</i> and <i>C</i>. cf. <i>caespitosa</i> occupy similar intertidal habitats to their respective, closely related counterparts <i>C. officinalis</i> and <i>C. caespitosa</i>. The results indicate cryptic diversity and suggest that there are many misidentified <i>Corallina</i> species. The paucity of South Atlantic studies of the Corallinales points to the need for a much greater taxonomic effort in this biogeographic region.
DOI: 10.6084/m9.figshare.12853569.v1
title: Morphology and molecular phylogeny of <i>Umbraulva</i> spp. (Ulvales, Ulvophyceae), and proposal of <i>Ryuguphycus</i> gen. nov. and <i>R. kuaweuweu</i> comb. nov.

description: The reproductive structures, early development and carotenoid composition of <i>Umbraulva kuaweuweu</i>, which is a deep-water ulvacean species recently reported from Hawaii, are reported for the first time using the specimens collected by dredging at ~30 m depth from Ogasawara Islands and Mageshima in southern Japan. Although <i>Umbraulva kuaweuweu</i> had siphonaxanthin as in <i>U. japonica</i>, a characteristic carotenoid of <i>Umbraulva, U. kuaweuweu</i> lacked α-carotene and β-carotene. <i>U. kuaweuweu</i> differed markedly from <i>U. japonica</i> in thallus morphology and reproductive structures: cell shape, presence/absence of inner rhizoidal filaments in the upper parts of thalli, solid/partly saccate thalli, and developmental processes of zoids. Multigene concatenated DNA sequences of chloroplast <i>rbc</i>L and <i>tuf</i>A genes, and nuclear 18S, 5.8S, 28S rDNA and rDNA ITS1 and ITS2 regions indicated separation of <i>U. kuaweuweu</i> from the clade comprised of the generitype <i>U. japonica, U. amamiensis, U. dangeardii</i> and <i>U. kaloakulau</i>. In conclusion, based on the distinctive morphology, life history, carotenoid composition and molecular phylogeny, we propose the establishment of a new genus <i>Ryuguphycus</i> gen. nov. to accommodate <i>U. kuaweuweu</i>, as <i>R. kuaweuweu</i> comb. nov.
DOI: 10.6084/m9.figshare.12853593
title: Taxonomic investigation of <i>Ralfsia</i>-like (Ralfsiales, Phaeophyceae) taxa in the North Atlantic Ocean based on molecular and morphological data, with descriptions of <i>Pseudoralfsiaceae fam</i>. nov., <i>Pseudoralfsia azorica</i> gen. et sp. nov. and <i>Nuchella vesicularis</i> gen. et sp. nov.

description: In this study we investigated <i>Ralfsia</i>-like crusts (i.e. excluding <i>Ralfsia</i> sensu stricto, <i>Stragularia</i> spp. and/or Scytosiphonaceae crustose phases) with an emphasis on the North Atlantic Ocean using molecular data (COI-5P, ITS and <i>rbc</i>L-3P) combined with morpho-anatomical comparisons of type material and contemporary specimens. Of the four species of <i>Ralfsia</i> previously reported in Europe, only <i>R. fungiformis</i> is presently recognized as belonging to <i>Ralfsia</i> sensu stricto, Ralfsiaceae, with the remaining species, <i>R. lucida, R. ovata</i> and <i>R. verrucosa</i>, of uncertain taxonomic status. Our study revealed 11 independent genetic lineages of <i>Ralfsia</i>-like taxa, which were not assignable to any of the recognized families of the Ralfsiales. To accommodate this diversity, we propose Pseudoralfsiaceae Parente, Fletcher & G.W.Saunders fam. nov., including two new genera <i>Pseudoralfsia</i> Parente, Fletcher & G.W.Saunders gen. nov. and <i>Nuchella</i> Parente, Fletcher & G.W.Saunders gen. nov. The first genus includes <i>Pseudoralfsia verrucosa</i> (Areschoug) Parente, Fletcher & G.W.Saunders comb. nov. as the generitype (the only species of the three remaining European species of uncertain taxonomic status assigned to <i>Ralfsia</i> that was reassessed here), <i>P. azorica</i> Parente, Fletcher & G.W.Saunders sp. nov. and seven undescribed genetic groups, which require additional vouchers for description. The second genus has as generitype <i>Nuchella vesicularis</i> Parente, Fletcher & G.W.Saunders sp. nov., and also includes <i>N</i>. sp._1MP, which was represented by a single sterile specimen from Swanage, UK and consequently not characterized. Three characteristics differentiate <i>Nuchella</i> from <i>Pseudoralfsia</i> – the former typically with numerous vesicles, non-synchronous development of the plurangia and hair pits arising from both middle and lower cells of the erect filaments. Species of Pseudoralfsiaceae can be distinguished from <i>Ralfsia</i> sensu stricto (Ralfsiaceae) mainly by DNA sequences and by consistently having frequent hair pits, and typically unsymmetrical thalli.
DOI: 10.6084/m9.figshare.12853593.v2
title: Taxonomic investigation of <i>Ralfsia</i>-like (Ralfsiales, Phaeophyceae) taxa in the North Atlantic Ocean based on molecular and morphological data, with descriptions of <i>Pseudoralfsiaceae fam</i>. nov., <i>Pseudoralfsia azorica</i> gen. et sp. nov. and <i>Nuchella vesicularis</i> gen. et sp. nov.

description: In this study we investigated <i>Ralfsia</i>-like crusts (i.e. excluding <i>Ralfsia</i> sensu stricto, <i>Stragularia</i> spp. and/or Scytosiphonaceae crustose phases) with an emphasis on the North Atlantic Ocean using molecular data (COI-5P, ITS and <i>rbc</i>L-3P) combined with morpho-anatomical comparisons of type material and contemporary specimens. Of the four species of <i>Ralfsia</i> previously reported in Europe, only <i>R. fungiformis</i> is presently recognized as belonging to <i>Ralfsia</i> sensu stricto, Ralfsiaceae, with the remaining species, <i>R. lucida, R. ovata</i> and <i>R. verrucosa</i>, of uncertain taxonomic status. Our study revealed 11 independent genetic lineages of <i>Ralfsia</i>-like taxa, which were not assignable to any of the recognized families of the Ralfsiales. To accommodate this diversity, we propose Pseudoralfsiaceae Parente, Fletcher & G.W.Saunders fam. nov., including two new genera <i>Pseudoralfsia</i> Parente, Fletcher & G.W.Saunders gen. nov. and <i>Nuchella</i> Parente, Fletcher & G.W.Saunders gen. nov. The first genus includes <i>Pseudoralfsia verrucosa</i> (Areschoug) Parente, Fletcher & G.W.Saunders comb. nov. as the generitype (the only species of the three remaining European species of uncertain taxonomic status assigned to <i>Ralfsia</i> that was reassessed here), <i>P. azorica</i> Parente, Fletcher & G.W.Saunders sp. nov. and seven undescribed genetic groups, which require additional vouchers for description. The second genus has as generitype <i>Nuchella vesicularis</i> Parente, Fletcher & G.W.Saunders sp. nov., and also includes <i>N</i>. sp._1MP, which was represented by a single sterile specimen from Swanage, UK and consequently not characterized. Three characteristics differentiate <i>Nuchella</i> from <i>Pseudoralfsia</i> – the former typically with numerous vesicles, non-synchronous development of the plurangia and hair pits arising from both middle and lower cells of the erect filaments. Species of Pseudoralfsiaceae can be distinguished from <i>Ralfsia</i> sensu stricto (Ralfsiaceae) mainly by DNA sequences and by consistently having frequent hair pits, and typically unsymmetrical thalli.
DOI: 10.6084/m9.figshare.12853566
title: Zinc-induced stress on cells of <i>Halamphora luciae</i> (Bacillariophyceae)

description: The aim of the present paper was to study the effect of zinc on the benthic diatom <i>Halamphora luciae</i>. Bioassays using the metal concentration corresponding to the IC<sub>zn</sub>50 were terminated in two selected endpoints: day 4 (acute phase) and day 9 (chronic phase). A 1.4- and 2.2-fold reduction in Zn<sup>2+</sup> with respect to the initial cation concentration was estimated by atomic absorption at days 4 and 9, respectively and was attributed both to precipitation of insoluble carbonates and oxides at alkaline pH and to biosorption. Carrying capacity of Zn-exposed cultures diminished even when growth rate remained unchanged, suggesting some degree of acclimation of the strain to metal stress. Zn stimulated cell bleaching together with the shift into the lipogenic phase, denoted by total lipid quantification and bigger lipid bodies volumes. Lipid peroxidation and phenolic compounds dosage revealed no major metal-induced oxidative stress. On the other hand, metal exposure had an antagonistic effect on the activation of alkaline phosphatase activity after phosphorus starvation. Superoxide dismutase (SOD) activity, cell motility and mucilage secretion were enhanced only during acute exposure. Soluble extracellular polymeric substances (S-EPS) from Zn-exposed cultures denoted a higher heterogeneity in monosaccharide composition. Frustules from metal-exposed cells presented a reduction in areolae diameter together with a lower area:volume ratio of the cells due to the addition of cingular bands.
DOI: 10.6084/m9.figshare.12853593.v1
title: Taxonomic investigation of Ralfsia-like (Ralfsiales, Phaeophyceae) taxa in the North Atlantic Ocean based on molecular and morphological data, with descriptions of Pseudoralfsiaceae fam. nov., Pseudoralfsia azorica gen. et sp. nov. and Nuchella vesicularis gen. et sp. nov.

description: In this study we investigated <i>Ralfsia</i>-like crusts (i.e. excluding <i>Ralfsia</i> sensu stricto, <i>Stragularia</i> spp. and/or Scytosiphonaceae crustose phases) with an emphasis on the North Atlantic Ocean using molecular data (COI-5P, ITS and <i>rbc</i>L-3P) combined with morpho-anatomical comparisons of type material and contemporary specimens. Of the four species of <i>Ralfsia</i> previously reported in Europe, only <i>R. fungiformis</i> is presently recognized as belonging to <i>Ralfsia</i> sensu stricto, Ralfsiaceae, with the remaining species, <i>R. lucida, R. ovata</i> and <i>R. verrucosa</i>, of uncertain taxonomic status. Our study revealed 11 independent genetic lineages of <i>Ralfsia</i>-like taxa, which were not assignable to any of the recognized families of the Ralfsiales. To accommodate this diversity, we propose Pseudoralfsiaceae Parente, Fletcher & G.W.Saunders fam. nov., including two new genera <i>Pseudoralfsia</i> Parente, Fletcher & G.W.Saunders gen. nov. and <i>Nuchella</i> Parente, Fletcher & G.W.Saunders gen. nov. The first genus includes <i>Pseudoralfsia verrucosa</i> (Areschoug) Parente, Fletcher & G.W.Saunders comb. nov. as the generitype (the only species of the three remaining European species of uncertain taxonomic status assigned to <i>Ralfsia</i> that was reassessed here), <i>P. azorica</i> Parente, Fletcher & G.W.Saunders sp. nov. and seven undescribed genetic groups, which require additional vouchers for description. The second genus has as generitype <i>Nuchella vesicularis</i> Parente, Fletcher & G.W.Saunders sp. nov., and also includes <i>N</i>. sp._1MP, which was represented by a single sterile specimen from Swanage, UK and consequently not characterized. Three characteristics differentiate <i>Nuchella</i> from <i>Pseudoralfsia</i> – the former typically with numerous vesicles, non-synchronous development of the plurangia and hair pits arising from both middle and lower cells of the erect filaments. Species of Pseudoralfsiaceae can be distinguished from <i>Ralfsia</i> sensu stricto (Ralfsiaceae) mainly by DNA sequences and by consistently having frequent hair pits, and typically unsymmetrical thalli.
DOI: 10.6084/m9.figshare.12853581.v1
title: Flagellar motility during sperm chemotaxis and phototaxis in fucalean algae

description: The swimming of male gametes of brown algae is controlled by two heterokont flagella, regardless of reproductive system, but the patterns of flagellar movement can vary. We investigated sperm chemotaxis and phototaxis in four oogamous Australasian fucalean species. Sperm morphology was similar to that of male gametes of isogamous and anisogamous species but their chemotactic behaviours were much closer to those of other oogamous species than those of isogamous and anisogamous species. Moreover, unlike anisogamous species, sperm chemotaxis of the oogamous species, <i>Hormosira banksii</i>, was not inhibited by a phosphodiesterase inhibitor, theophylline, suggesting variability in regulatory mechanisms of sperm chemotaxis amongst brown algae. In our previous work, lower extracellular Ca<sup>2+</sup>, supernatant of female gametes and theophylline induced the reversal of phototactic signs in anisogamous male gametes. In contrast, lower extracellular Ca<sup>2+</sup> and theophylline did not significantly affect phototaxis in sperm of the oogamous <i>H. banksii</i>. Moreover, high extracellular Ca<sup>2+</sup> and supernatant of eggs disrupted the negative phototaxis but did not reverse their phototactic sign. These results suggest that the regulation of phototaxis also varies amongst brown algal gametes. Further investigation of brown algal species is warranted to assess the generality of these patterns of chemotaxis and phototaxis among anisogamous and oogamous gametes.