DOI: 10.5061/dryad.8582
title: Data from: An integrative approach to delimiting species in a rare but widespread mycoheterotrophic orchid.

description: In the spirit of recent calls for species delimitation studies to become
 more pluralistic, incorporating multiple sources of evidence, we adopted
 an integrative, phylogeographic approach to delimiting species and
 evolutionarily significant units (ESU) in the Corallorhiza striata species
 complex. This rare, North American, mycoheterotrophic orchid has been a
 taxonomic challenge with regard to species boundaries, displaying complex
 patterns of variation and reduced vegetative morphology. We employed
 plastid DNA, nuclear DNA, and morphometrics, treating the C. striata
 complex as a case study for integrative species delimitation. We found
 evidence for differentiation of the endangered C. bentleyi (eastern USA) +
 C. striata var. involuta (Mexico) from the remaining C. striata (= C.
 striata s.s.; USA, Canada, Mexico). Corallorhiza striata involuta and C.
 bentleyi, disjunct by thousands of kilometers (Mexico-Appalachia), were
 genetically identical but morphologically distinct. In light of these
 findings, the C. striata complex represents three species: C. bentleyi, C.
 involuta, and a widespread C. striata s.s under the phylogenetic species
 concept. Bayesian coalescent estimation delimited four species, but more
 informative loci and a definitive species tree will be needed to place
 higher confidence in future analyses. Three groupings were identified
 within C. striata s.s., corresponding to C. striata striata, C. striata
 vreelandii, and Californian accessions, but these were not delimited as
 phylogenetic species. Each, however, comprises an ESU, based on morphology
 and DNA sequences from two genomes, warranting conservation
 considerations.
description: plastid_comb_mcicPlastid DNA sequence matrix: interleaved nexus alignment including rbcL and rpl32-trnL plastid spacer sequences, along with coded insertion/deletion characters. Data are in both standard DNA format (ACGT) and binary (0,1) format. Data collected by Craig Barrett [cfb66@cornell.edu].F3HnewInterleaved nexus alignment file including Flavanone 3-hydroxylase intron sequences. Data are in standard DNA format (ACGT)format. Data collected by Craig Barrett [cfb66@cornell.edu].5p_RPB2Interleaved nexus alignment file including the 5' region of RNA Polymerase II subunit 2 intron 23 for Corallorhiza striata complex. Data are in standard DNA format (ACGT). Data collected by Craig Barrett [cfb66@cornell.edu].3p_RPB2Interleaved nexus alignment file including the 3' region of RNA Polymerase II subunit 2 intron 23 for Corallorhiza striata complex. Data are in standard DNA format (ACGT). Data collected by Craig Barrett [cfb66@cornell.edu].ITSInterleaved nexus alignment file including the Internal Transcribed Spacer region for Corallorhiza striata complex. Data are in standard DNA format (ACGT). Data collected by Craig Barrett [cfb66@cornell.edu].C.striata_morphology_11_15_10Floral morphometric measurement data (units = mm) for the Corallorhiza striata complex. Column headings correspond to each individual measured floral part. Row labels correspond to voucher numbers for each individual. Data collected by Craig Barrett [cfb66@cornell.edu].C.striata_landmarksMicrosoft Excel spreadsheet containing raw coordinate data of labellum landmarks for the Corallorhiza striata complex In the first row are labels of each landmark (each appears twice, next each other). The row below these are the the X and Y-coordinate labels corresponding to each landmark (e.g. X2, Y2). Data represent positions (i.e. coordinates) on a pixel grid, and are not standard units of measurement. Row labels are voucher numbers for individuals. Data were colleced by Craig Barrett [cfb66@cornell.edu].