cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25934/pr00007744
title: Inferential reproducibility of therapeutic research: a registered report for a cross-sectional study of RCTs available on major data-sharing platforms 2

description: Randomized Controlled Trials (RCTs) are of major importance in providing information about health practices and policies. Ideally, the general public and scientists feel more confident when their methods and results can be reproduced. This registered report introduces a cross-sectional study aiming to explore inferential reproducibility (i.e. Individual Patient Data is available and qualitatively similar conclusions can be drawn from a re-analysis of the original trials) for RCTs, for which there is available data on major data-sharing platforms. 
This study will include RCTs identified on 3 repositories (Clinical Study Data Request, VIVLI and Yale University Open Data Access). Eligible RCTs will be phase III studies conducted in the field of therapeutics. 62 of these studies will be randomly sampled, ensuring a precision of ± 7.5% to estimate our primary outcome, i.e. the proportion of studies where the conclusions are reproduced (we hypothesize that more than 90 % of studies could be reproduced). One researcher will then retrieve the Individual Patient Data for these studies and other necessary documents for re-analysis by contacting the platforms and the study sponsors. For each study he will prepare a dossier containing the Individual Patient Data (IPD), the protocol and information on the conduct of the study. A second researcher who will have no access to study reports (including publications) or analytical codes will use the dossier to run an independent reanalysis of each trial. All results from these re-analyses will be reported in terms of the conclusions, p-values, effect sizes and changes from the initial protocol in each study.  Then a team of two researchers not involved in the re-analysis will compare results of the re-analysis with the published results of the trial. In case of conflicting results, a statistician will re-analyse the data independently before concluding to a lack of inferential reproducibility. Key issues that arose in the course of data requests and reanalysis will be qualitatively described
This registered report is part of a wider project called “Reproducibility in Therapeutic  Research”, funded by the Agence Nationale de la Recherche, and a complementary registered report aims to explore the reproducibility of trials used for decision-making on Marketing Authorizations for new medicines in the European Medicine Agency
DOI: 10.5517/ccdc.csd.cc1zyxxr
title: CCDC 1846016: Experimental Crystal Structure Determination

description: Related Article: Ilya P. Filippov, Anastasiya V. Agafonova, Gleb D. Titov, Ilia A. Smetanin, Nikolai V. Rostovskii, Alexander F. Khlebnikov, Mikhail S. Novikov|2022|J.Org.Chem.|87|6514|doi:10.1021/acs.joc.2c00514
DOI: 10.5517/ccdc.csd.cc27ddbd
title: CCDC 2067493: Experimental Crystal Structure Determination

description: Related Article: Manoj Kumar, Shalini Verma, Vivek Mishra, Oliver Reiser, Akhilesh K. Verma|2022|J.Org.Chem.|87|6263|doi:10.1021/acs.joc.2c00491
DOI: 10.5517/ccdc.csd.cc27ddcf
title: CCDC 2067494: Experimental Crystal Structure Determination

description: Related Article: Manoj Kumar, Shalini Verma, Vivek Mishra, Oliver Reiser, Akhilesh K. Verma|2022|J.Org.Chem.|87|6263|doi:10.1021/acs.joc.2c00491
DOI: 10.5517/ccdc.csd.cc27v4x5
title: CCDC 2080717: Experimental Crystal Structure Determination

description: Related Article: Jogendra Kumar, Sukalyan Bhadra|2021|CSD Communication|||
description: Related Article: Jogendra Kumar, Anupam Kumar Singh, Aniket Gupta, Sukalyan Bhadra|2022|J.Org.Chem.|87|6330|doi:10.1021/acs.joc.1c02935
DOI: 10.5517/ccdc.csd.cc2855n9
title: CCDC 2090350: Experimental Crystal Structure Determination

description: Related Article: Xiaoling Liu, Haimei Liu, Chang Bian, Ke-Hu Wang, Junjiao Wang, Danfeng Huang, Yingpeng Su, Xiaobo Lv, Yulai Hu|2022|J.Org.Chem.|87|5882|doi:10.1021/acs.joc.2c00176
DOI: 10.5517/ccdc.csd.cc28gdbh
title: CCDC 2099206: Experimental Crystal Structure Determination

description: Related Article: Wenjun Yang, Tejas Y. Kalavalapalli, Annika M. Krieger, Taras A. Khvorost, Ivan Yu. Chernyshov, Manuela Weber, Evgeny A. Uslamin, Evgeny A. Pidko, Georgy A. Filonenko|2022|J.Am.Chem.Soc.|144|8129|doi:10.1021/jacs.2c00548
DOI: 10.5517/ccdc.csd.cc28gdcj
title: CCDC 2099207: Experimental Crystal Structure Determination

description: Related Article: Wenjun Yang, Tejas Y. Kalavalapalli, Annika M. Krieger, Taras A. Khvorost, Ivan Yu. Chernyshov, Manuela Weber, Evgeny A. Uslamin, Evgeny A. Pidko, Georgy A. Filonenko|2022|J.Am.Chem.Soc.|144|8129|doi:10.1021/jacs.2c00548
DOI: 10.5517/ccdc.csd.cc28gddk
title: CCDC 2099208: Experimental Crystal Structure Determination

description: Related Article: Wenjun Yang, Tejas Y. Kalavalapalli, Annika M. Krieger, Taras A. Khvorost, Ivan Yu. Chernyshov, Manuela Weber, Evgeny A. Uslamin, Evgeny A. Pidko, Georgy A. Filonenko|2022|J.Am.Chem.Soc.|144|8129|doi:10.1021/jacs.2c00548
DOI: 10.5517/ccdc.csd.cc28t46g
title: CCDC 2109525: Experimental Crystal Structure Determination

description: Related Article: Ilya P. Filippov, Anastasiya V. Agafonova, Gleb D. Titov, Ilia A. Smetanin, Nikolai V. Rostovskii, Alexander F. Khlebnikov, Mikhail S. Novikov|2022|J.Org.Chem.|87|6514|doi:10.1021/acs.joc.2c00514
DOI: 10.5517/ccdc.csd.cc28t4fp
title: CCDC 2109532: Experimental Crystal Structure Determination

description: Related Article: Ilya P. Filippov, Anastasiya V. Agafonova, Gleb D. Titov, Ilia A. Smetanin, Nikolai V. Rostovskii, Alexander F. Khlebnikov, Mikhail S. Novikov|2022|J.Org.Chem.|87|6514|doi:10.1021/acs.joc.2c00514
DOI: 10.5517/ccdc.csd.cc28t4hr
title: CCDC 2109534: Experimental Crystal Structure Determination

description: Related Article: Ilya P. Filippov, Anastasiya V. Agafonova, Gleb D. Titov, Ilia A. Smetanin, Nikolai V. Rostovskii, Alexander F. Khlebnikov, Mikhail S. Novikov|2022|J.Org.Chem.|87|6514|doi:10.1021/acs.joc.2c00514
DOI: 10.5517/ccdc.csd.cc28yw6b
title: CCDC 2114082: Experimental Crystal Structure Determination

description: Related Article: Renyu Guo, Yu-Che Chang, Loic Herter, Christophe Salome, Sarah E. Braley, Thomas C. Fessard, M. Kevin Brown|2022|J.Am.Chem.Soc.|144|7988|doi:10.1021/jacs.2c02976
DOI: 10.5517/ccdc.csd.cc299n4f
title: CCDC 2124434: Experimental Crystal Structure Determination

description: Related Article: Jogendra Kumar|2021|CSD Communication|||
description: Related Article: Jogendra Kumar, Anupam Kumar Singh, Aniket Gupta, Sukalyan Bhadra|2022|J.Org.Chem.|87|6330|doi:10.1021/acs.joc.1c02935
DOI: 10.5517/ccdc.csd.cc28ykzs
title: CCDC 2113796: Experimental Crystal Structure Determination

description: Related Article: Weiqing Lu, Hui Wang, Weigang Fan, Shengyu Dai|2022|Inorg.Chem.|61|6799|doi:10.1021/acs.inorgchem.1c03969
DOI: 10.5517/ccdc.csd.cc27212g
title: CCDC 2057534: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc27213h
title: CCDC 2057535: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc29dn9p
title: CCDC 2127322: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc29dncr
title: CCDC 2127324: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc29dnds
title: CCDC 2127325: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc29wnpk
title: CCDC 2141749: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnql
title: CCDC 2141750: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnsn
title: CCDC 2141752: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wntp
title: CCDC 2141753: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnzv
title: CCDC 2141758: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wp0x
title: CCDC 2141759: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wp2z
title: CCDC 2141761: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29x0t2
title: CCDC 2142094: Experimental Crystal Structure Determination

description: Related Article: Zhou Tong, Xinju Peng, Lifen Peng, Wei Deng, Zhiqing Wang, Hao Lu, Weijun Yang, Shuang-Feng Yin, Nobuaki Kambe, Renhua Qiu|2022|J.Org.Chem.|87|6064|doi:10.1021/acs.joc.2c00325
DOI: 10.5517/ccdc.csd.cc29wzbj
title: CCDC 2142048: Experimental Crystal Structure Determination

description: Related Article: Zhou Tong, Xinju Peng, Lifen Peng, Wei Deng, Zhiqing Wang, Hao Lu, Weijun Yang, Shuang-Feng Yin, Nobuaki Kambe, Renhua Qiu|2022|J.Org.Chem.|87|6064|doi:10.1021/acs.joc.2c00325
DOI: 10.5517/ccdc.csd.cc29wz4b
title: CCDC 2142042: Experimental Crystal Structure Determination

description: Related Article: Zhou Tong, Xinju Peng, Lifen Peng, Wei Deng, Zhiqing Wang, Hao Lu, Weijun Yang, Shuang-Feng Yin, Nobuaki Kambe, Renhua Qiu|2022|J.Org.Chem.|87|6064|doi:10.1021/acs.joc.2c00325
DOI: 10.5517/ccdc.csd.cc21q161
title: CCDC 1898012: Experimental Crystal Structure Determination

description: Related Article: Pablo Hermosilla, Pilar García-Orduña, Pablo J. Sanz Miguel, Víctor Polo, Miguel A. Casado|2022|Inorg.Chem.|61|7120|doi:10.1021/acs.inorgchem.2c00617
DOI: 10.5517/ccdc.csd.cc2b59hb
title: CCDC 2150051: Experimental Crystal Structure Determination

description: Related Article: Ci-Ci Sun, Wang Li, De-Xuan Liu, Zi-Yi Du, Chun-Ting He, Wei-Xiong Zhang, Xiao-Ming Chen|2022|Inorg.Chem.|61|7201|doi:10.1021/acs.inorgchem.2c00886
DOI: 10.5517/ccdc.csd.cc29wnnj
title: CCDC 2141748: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnrm
title: CCDC 2141751: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnvq
title: CCDC 2141754: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnwr
title: CCDC 2141755: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnxs
title: CCDC 2141756: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wnyt
title: CCDC 2141757: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wp1y
title: CCDC 2141760: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc29wp30
title: CCDC 2141762: Experimental Crystal Structure Determination

description: Related Article: Chun-Fai Ng, Hak-Fun Chow, Thomas C. W. Mak|2022|Cryst.Growth Des.|22|3365|doi:10.1021/acs.cgd.2c00164
DOI: 10.5517/ccdc.csd.cc27214j
title: CCDC 2057536: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc27215k
title: CCDC 2057537: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc29dnbq
title: CCDC 2127323: Experimental Crystal Structure Determination

description: Related Article: Mei-Yan Gao, Zirui Wang, Qiao-Hong Li, Dejing Li, Yayong Sun, Yassin H. Andaloussi, Chao Ma, Chenghua Deng, Jian Zhang, Lei Zhang|2022|J.Am.Chem.Soc.|144|8153|doi:10.1021/jacs.2c00765
DOI: 10.5517/ccdc.csd.cc2b59f8
title: CCDC 2150049: Experimental Crystal Structure Determination

description: Related Article: Ci-Ci Sun, Wang Li, De-Xuan Liu, Zi-Yi Du, Chun-Ting He, Wei-Xiong Zhang, Xiao-Ming Chen|2022|Inorg.Chem.|61|7201|doi:10.1021/acs.inorgchem.2c00886
DOI: 10.5517/ccdc.csd.cc2b59g9
title: CCDC 2150050: Experimental Crystal Structure Determination

description: Related Article: Ci-Ci Sun, Wang Li, De-Xuan Liu, Zi-Yi Du, Chun-Ting He, Wei-Xiong Zhang, Xiao-Ming Chen|2022|Inorg.Chem.|61|7201|doi:10.1021/acs.inorgchem.2c00886
DOI: 10.25934/pr00003474.1
title: Available IPD datapackage for study 'Efficacy {FEV1, COPD Exacerbations & HRQoL} & Safety of 5mcg Tiotropium Respimat in COPD'

description: The objective of the study is to evaluate the long-term (one year) efficacy and safety of tiotropium delivered by the Respimat inhaler in patients with COPD. Specifically, the study will examine the effect of treatment on COPD exacerbations.
DOI: 10.25934/pr00003541.1
title: Available IPD datapackage for study 'Randomised, Double-blind, Placebo-controlled, Parallel Group Study to Assess the Efficacy and Safety of 48 Weeks of Once Daily Treatment of Orally Inhaled BI 1744 CL (5 mcg [2 Actuations of 2.5 mcg] and 10 mcg [2 Actuations of 5 mcg]) Delivered by the Respimat® Inhaler, in Patients With Chronic Obstructive Pulmonary Disease (COPD)'

description: This primary objective of this study is to compare two doses of BI 1744 CL inhalation solution delivered by the Respimat® inhaler once daily to placebo in patients with chronic obstructive pulmonary disease (COPD). The safety of BI 1744 CL inhalation solution delivered through the Respimat inhaler will also be compared to placebo.
DOI: 10.5281/zenodo.6545219
title: Dataset for "Sensitivity of subregional distribution of socioeconomic conditions to the global assessment of water scarcity"

description: This dataset contains the data related to the final analysis for "Sensitivity of subregional distribution of socioeconomic conditions to the global assessment of water scarcity".
DOI: 10.5281/zenodo.6545218
title: Dataset for "Sensitivity of subregional distribution of socioeconomic conditions to the global assessment of water scarcity"

description: This dataset contains the data related to the final analysis for "Sensitivity of subregional distribution of socioeconomic conditions to the global assessment of water scarcity".
DOI: 10.48512/xcv8468830
title: Dataset of Antemortem Tooth Loss and Carious Lesions at Deir el-Medina

description: These data are from observations on antemortem tooth loss and the presence of carious lesions from the site of Deir el-Medina, Egypt. The data were collected by Anne Austin and Mélie Louys as part of the mission of the Institut Français d’Archéologie Orientale at the site during the 2012-14 and 2019-20 field seasons. These data were derived from human remains found in Theban Tombs 6, 217, 290, and 298. They are estimated to date from 1550-945 BCE. These data include macroscopic observations from 32 mandibles and 44 maxillae consisting of 485 observable teeth and 1,052 observable tooth sockets. Any mandibles or maxillae with deciduous dentition present or unerupted permanent dentition are excluded. Estimations of sex are included for each element. Sex estimation is based on cranial and pelvic morphology (Buikstra and Ubelaker, 1994; Walker, 2005). These estimations include the following categories: Unknown, Female – Definitely, Female – Possibly, Ambiguous, Male – Possibly, and Male – Definitely. Given the degree of commingling, however, sex estimates were not always feasible. Isolated mandibles are not assigned sex estimations as the mental eminence alone is not consistently reliable for sex estimation (Lewis and Garvin, 2016). Estimations of age are also provided. Age estimation is based on observations of cranial suture closure (Buikstra and Ubelaker, 1994), the pubic symphysis (Brooks and Suchey, 1990; Todd, 1921a, 1921b), epiphyseal fusion (Scheuer and Black, 2004), and the auricular surface (Buckberry and Chamberlain, 2002) when observable. These estimates are then categorized as unknown, under 20 years old, 20-35 years old, 36-50 years old, and over 50 years old. As mandibles were often found with no articulating crania, many do not have an age and/or sex estimation. All mandibles and maxillae were observed for antemortem tooth loss (AMTL) and the presence of carious lesions. For each element, the number of teeth or tooth sockets showing the absence or presence of AMTL is listed. Tooth sockets were noted as having the presence of AMTL if they showed evidence of partial or full healing. The number of teeth with carious lesions absent or present are also provided for each element. Carious lesions were marked as present only if “a stained, irregular walled cavity is evident from visual inspection” (Steckel et al., 2019, p. 406). All identifications were made without the aid of a microscope or radiographs. This definition excludes precavitated carious lesions (i.e., any detected through discoloration of enamel alone) as well as early pit and fissure caries which can be difficult to distinguish due to morphological variation in molar cusps.
DOI: 10.5281/zenodo.6542674
title: Sequences of Orthorectified Images of the Water Surface of Two Rivers: River Sheaf (Sheffield, UK), and River Calder (Todmorden, UK)

description: This data set contains sequences of orthorectified images of the free surface of two rivers: River Sheaf, Sheffield, United Kingdom (Latitude: 53.373056° Longitude: -1.463913° (WGS 84)), recorded between January 2019 and February 2020; River Calder, Todmorden, United Kingdom (Latitude: 53.716198° Longitude: -2.097028°), recorded between 6 and 13 October 2020. The images are extracted from videos of the water surface also included in the data set. They are complemented by Matlab files containing the corresponding average space-time Fourier spectra. Each measurement is associated with the corresponding measurement of the river depth and estimates of the discharge. Please refer to Documentation.pdf for further details.
DOI: 10.25934/pr00005221.1
title: Available IPD datapackage for study 'A Randomised, Double-blind, Placebo-controlled, Parallel-group Trial to Evaluate Efficacy and Safety of Tiotropium Inhalation Solution (2.5 mcg and 5 mcg) Delivered Via Respimat® Inhaler Once Daily in the Evening Over 48 Weeks in Children (6 to 11 Years Old) With Moderate Persistent Asthma'

description: The overall purpose of the trial is to evaluate efficacy and safety of tiotropium inhalation solution delivered via Respimat® inhaler (2.5 mcg and 5 mcg once daily in the evening) over 48 weeks, compared to placebo, in children (6 to 11 years old) with moderate persistent asthma.
DOI: 10.6084/m9.figshare.17089196
title: Metabarcoding analysis of marine zooplankton confirms the ecological role of a sheltered bight along an exposed continental shelf.

description: Zooplankton plays an essential role in marine ecosystems as the link between primary producers (phytoplankton) and higher trophic levels in food webs, and as a dynamic pool of recruits for invertebrates and fish. Zooplankton communities are diverse with a patchy distribution at different spatial scales, influenced by oceanographic processes. The continental shelf of eastern South Africa is narrow and exposed to the western-boundary Agulhas Current, with some shelter against strong directional flow provided by the broader KwaZulu-Natal (KZN) Bight, a coastal offset adjacent to an estuary. We compared zooplankton species richness, diversity and relative abundance of key taxa among sheltered and exposed shelf areas using metabarcoding and community analysis, to explore the ecological role of the bight in a highly dynamic ocean region. Metabarcoding recovered higher richness and diversity at a finer resolution than could previously be achieved with traditional microscopy. Of 271 operational taxonomic units (OTUs) recovered through metabarcoding, 63% could be matched with &gt;95% sequence similarity to reference barcodes. OTUs were dominated by malacostracan crustaceans (161 spp.), ray-finned fishes (45 spp.) and copepods (28 spp.). Species richness, diversity and the relative abundance of key taxa differed between sheltered and exposed shelf areas. Lower species richness in the bight was partly attributed to structurally homogenous benthic habitats, and an associated reduction of meroplanktonic species originating from local benthic-pelagic exchange. High relative abundance of a ray-finned fish in the bight, as observed fish eggs and read counts, confirmed that the bight is an important fish spawning area. Overall, zooplankton metabarcoding outputs were congruent with findings of earlier ecological research using more traditional methods of observation.