cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.26008/1912/bco-dmo.907393.1
title: Daily growth data of the Karlodinium veneficum growth experiment conducted in the Horn Point Laboratory between June 2021- January 2022.

description: Using a multifactorial design, we studied the interactive effects of temperature (15, 20, 25, 28 and 30 0C), salinity (5, 10, 15, 20 and 30) and light (low-100 and high-300 μmol photons m−2 s−1) on growth, thermal niche properties and cellular carbon (C) and nitrogen (N) of the toxic dinoflagellate, Karlodinium veneficum, originated from the Chesapeake Bay. Here we report the daily growth data measured as raw fluorescence at each experimental combination  in quadruplicates and the C and N cell quota data measured in the final samples for each experimental combination in duplicates.
DOI: 10.26008/1912/bco-dmo.907463.1
title: Cellular carbon (C) and nitrogen (N) data of the Karlodinium veneficum growth experiment conducted in the Horn Point Laboratory between June 2021- January 2022.

description: Using a multifactorial design, we studied the interactive effects of temperature (15, 20, 25, 28 and 30 0C), salinity (5, 10, 15, 20 and 30) and light (low-100 and high-300 μmol photons m−2 s−1) on growth, thermal niche properties and cellular carbon (C) and nitrogen (N) of the toxic dinoflagellate, Karlodinium veneficum, originated from the Chesapeake Bay. Here we report the daily growth data measured as raw fluorescence at each experimental combination  in quadruplicates and the C and N cell quota data measured in the final samples for each experimental combination in duplicates.
DOI: 10.26008/1912/bco-dmo.906317.1
title: Kelp growth data from seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species

description: This kelp growth dataset results from two, month-long global change laboratory experiments conducted in Sitka, Alaska from February 12 to March 18, 2020 (&quot;winter&quot; experiment) and August 15 to September 16, 2020 (&quot;summer&quot; experiment). These experiments were used to tease apart the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species (Macrocystis pyrifera, Hedophyllum nigripes, and Neoagarum fimbriatum) to pH and temperature. Experimental controls were designed to approximate current environmental conditions in Sitka Sound, and treatments were based on projected end-of-century scenarios of ocean acidification (OA) and warming (OW) for this region. At the end of the experiments, the investigators assessed the seasonal impact of OW and OA on kelp growth rates, thallus nitrogen content, and carbon acquisition strategy based on thallus δ13C values. Finally, to test whether kelp palatability was impacted by future warming and acidification, tissue of H. nigripes and N. fimbriatum grown during the experiments was used to perform feeding assays with a common kelp forest consumer. 

These experiments were performed to improve our understanding of how global change will alter marine primary producer resources by integrating natural variation in environmental drivers. This project was overseen by Dr. Kristy Kroeker and her Ph.D. student Lauren Bell, both at the University of California Santa Cruz. Experiments took place in the basement laboratory at the Sitka Sound Science Center in Sitka, AK. This work was supported by the National Science Foundation (OCE-1752600), the David and Lucile Packard Foundation, the North Pacific Research Board's Graduate Student Research Award (1748-02), the UCSC Physical and Biological Sciences 2019 Future Leaders in Coastal Science Award, the Seymour Marine Discovery Center's Student Research and Education Award, and the California State University Monterey Bay Undergraduate Research Opportunities Center.
DOI: 10.26008/1912/bco-dmo.906416.1
title: Results from feeding assays conducted as part of seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species

description: These data are from feeding assays determined during two, month-long global change laboratory experiments conducted in Sitka, Alaska from February 12 to March 18, 2020 (&quot;winter&quot; experiment) and August 15 to September 16, 2020 (&quot;summer&quot; experiment). These experiments were used to tease apart the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species (Macrocystis pyrifera, Hedophyllum nigripes, and Neoagarum fimbriatum) to pH and temperature. Experimental controls were designed to approximate current environmental conditions in Sitka Sound, and treatments were based on projected end-of-century scenarios of ocean acidification (OA) and warming (OW) for this region. At the end of the experiments, the investigators assessed the seasonal impact of OW and OA on kelp growth rates, thallus nitrogen content, and carbon acquisition strategy based on thallus δ13C values. Finally, to test whether kelp palatability was impacted by future warming and acidification, tissue of H. nigripes and N. fimbriatum grown during the experiments was used to perform feeding assays with a common kelp forest consumer. 

These experiments were performed to improve our understanding of how global change will alter marine primary producer resources by integrating natural variation in environmental drivers. This project was overseen by Dr. Kristy Kroeker and her Ph.D. student Lauren Bell, both at the University of California Santa Cruz. Experiments took place in the basement laboratory at the Sitka Sound Science Center in Sitka, AK. This work was supported by the National Science Foundation (OCE-1752600), the David and Lucile Packard Foundation, the North Pacific Research Board's Graduate Student Research Award (1748-02), the UCSC Physical and Biological Sciences 2019 Future Leaders in Coastal Science Award, the Seymour Marine Discovery Center's Student Research and Education Award, and the California State University Monterey Bay Undergraduate Research Opportunities Center.
DOI: 10.26008/1912/bco-dmo.845039.2
title: Coral associated microbes on coral, sediment, and water sampled from coral reefs in Mo'orea, French Polynesia from 2017 to 2020

description: Three species of coral, plus water and sediment, were sampled at 21 sites around the island of Mo’orea, French Polynesia during the dry and rainy seasons during 2017 to 2020.  Coral associated microbes (bacteria and archaea) were investigated and their community composition characterized through sequencing of the 16S rRNA gene.
DOI: 10.26008/1912/bco-dmo.906469.1
title: Carbon and nitrogen data from kelp determined during seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species

description: These carbon and nitrogen data from dried kelp were determined during two, month-long global change laboratory experiments conducted in Sitka, Alaska from February 12 to March 18, 2020 (&quot;winter&quot; experiment) and August 15 to September 16, 2020 (&quot;summer&quot; experiment). These experiments were used to tease apart the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species (Macrocystis pyrifera, Hedophyllum nigripes, and Neoagarum fimbriatum) to pH and temperature. Experimental controls were designed to approximate current environmental conditions in Sitka Sound, and treatments were based on projected end-of-century scenarios of ocean acidification (OA) and warming (OW) for this region. At the end of the experiments, the investigators assessed the seasonal impact of OW and OA on kelp growth rates, thallus nitrogen content, and carbon acquisition strategy based on thallus δ13C values. Finally, to test whether kelp palatability was impacted by future warming and acidification, tissue of H. nigripes and N. fimbriatum grown during the experiments was used to perform feeding assays with a common kelp forest consumer. 

These experiments were performed to improve our understanding of how global change will alter marine primary producer resources by integrating natural variation in environmental drivers. This project was overseen by Dr. Kristy Kroeker and her Ph.D. student Lauren Bell, both at the University of California Santa Cruz. Experiments took place in the basement laboratory at the Sitka Sound Science Center in Sitka, AK. This work was supported by the National Science Foundation (OCE-1752600), the David and Lucile Packard Foundation, the North Pacific Research Board's Graduate Student Research Award (1748-02), the UCSC Physical and Biological Sciences 2019 Future Leaders in Coastal Science Award, the Seymour Marine Discovery Center's Student Research and Education Award, and the California State University Monterey Bay Undergraduate Research Opportunities Center.
DOI: 10.26008/1912/bco-dmo.904721.1
title: Bottle-calibrated dissolved oxygen profiles from yearly turn-around cruises for the Ocean Observations Initiative (OOI) Irminger Sea Array 2014 – 2022

description: This dataset contains bottle-calibrated dissolved oxygen (DO) profiles collected from Conductivity Temperature Depth (CTD) casts on turn-around cruises performed yearly to maintain the Ocean Observations Initiative (OOI) Global Irminger Sea Array (60.46°N, 38.44°W). DO profiles were used in conjunction with oxygen bottle measurements (Winklers) to produce a post-cruise oxygen-calibrated CTD product for scientific use. Bottle-calibrated CTD salinity products were used to produce post-cruise oxygen-calibrated CTD profiles starting in 2018 (Year 5). This document contains overviews of CTD data collection and processing and post-processing oxygen sensor calibration method. Reports for each cruise include a summary of relevant cruise events, oxygen sensor calibration results, and issues/problems associated with oxygen data collected on each cruise. This dataset has been created for end-users that require field-calibrated oxygen data products that are currently not provided by OOI through its standard data dissemination.
DOI: 10.26008/1912/bco-dmo.904722.1
title: Supplementary discrete sample measurements of dissolved oxygen, dissolved inorganic carbon, and total alkalinity from Ocean Observatories Initiative (OOI) cruises to the Irminger Sea Array 2018-2019

description: This dataset contains discrete sample measurements of dissolved oxygen, dissolved inorganic carbon, and total alkalinity collected during yearly Ocean Observatories Initiative (OOI) turn-around cruises to maintain the Irminger Sea Array (60.46°N, 38.44°W). Samples in this dataset were collected as part of an ancillary research project that joined the OOI turn-around cruises in June 2018 and August 2019 as part of ongoing efforts to enable OOI biogeochemical sensor data to be used to address scientific questions about ocean carbon cycling and the biological carbon pump. 

Discrete sample data collected and analyzed by this research team complement data collected by the OOI program as part of routine turn-around cruise activities. We provide the supplementary measurements made by our team alongside salinity- and oxygen- calibrated Conductivity Temperature Depth (CTD) and oxygen sensor data from the depths where Niskin bottles were closed for sample collection and additional discrete oxygen measurements made by the OOI team.
DOI: 10.26008/1912/bco-dmo.905306.1
title: Transcriptomes of co-cultured marine microbes (Emiliania huxleyi, Thalassiosira pseudonana, and Synechococcus) and Ruegeria pomeroyi DSS-3

description: These data are from three laboratory studies of co-cultured temperature-acclimated marine microbes and Ruegeria pomeroyi DSS-3.

Model systems were established in which temperature-acclimated microbial strains were co-cultured with the heterotrophic bacterium Ruegeria pomeroyi. Cultures of the coccolithophore Emiliania huxleyi CCMP151 were pre-acclimated to three temperature treatments (15°, 20°, and 28° Celsius (C)). Cultures of the diatom Thalassiosira pseudonana CCMP1335 were pre-acclimated to 14°, 20°, and 28°C. Cultures of Synechococcus sp. WH8102 were pre-acclimated to 20°, 24°, and 28°C.

The investigators characterized the transcriptomes of both the phytoplankter and heterotrophic bacterium at initial and late exponential growth at the three temperatures.
DOI: 10.26008/1912/bco-dmo.855139.4
title: DOP concentration observations from the global ocean between 1990 and 2021 (DOP N2 fixation and export production project)

description: DOP (Dissolved Organic Phosphorus) concentration observations in the global ocean between 1990 and 2021 over multiple deployments. DOP concentrations in this study were calculated as the difference between TDP concentrations and SRP concentrations of samples. For DOP concentrations reported here, TDP concentrations were measured by the ash/hydrolysis method based on Monaghan and Ruttenberg, 1999.
DOI: 10.26008/1912/bco-dmo.906070.1
title: Fluorescence dissolved organic matter (fDOM) analysis of inhalant and exhalent water samples from sponges studied at an artificial reef off the coast of the Florida Keys, USA in August 2021

description: Coral reefs are biodiverse and productive ecosystems that are found in typically oligotrophic (low nutrient) environments. The focus of research on nutrient acquisition and partitioning among coral reef organisms has, historically, been focused on corals and their symbiotic zooxanthellae. For example, different clades of zooxanthellae are adapted to different irradiance levels which facilitates coexisting species of corals. Comparatively very few studies have asked if and how coexisting sponges and their symbiotic microbial communities partition nutrients (i.e., utilization of different nutrient pools across species). To address this question, this study set up an artificial reef off the coast of the Florida Keys using dominant emergent sponges found in the Caribbean and Florida Keys. Inhalant and exhalent water samples were collected using Vacusip. Samples were filtered with a 0.2-micrometer supor filter and then processed for dissolved nutrients using fluorescence dissolved organic matter (fDOM). This is the first application of fDOM analysis to sponges. 

In this study, we found that the microbial abundance (i.e. the commonly used high and low microbial abundance (HMA/LMA) classification) was not an effective indicator for the way in which nutrients are processed by these sponge species. Additionally, the use of fDOM analysis indicated nuance in DOM utilization across species with differential consumption of fDOM components across sponge species. In summary, rather than microbial abundance alone, a combination of sponge species identity and the composition of the symbiotic microbial community members (e.g., presence of photosymbionts) appears to explain the most variation in nutrient processing by sponges. These results provide the first support for resource partitioning of dissolved nutrients across coexisting sponge species and provide support for the evolutionary importance of microbial communities in sponges.
DOI: 10.26008/1912/bco-dmo.880924.1
title: Population changes in Halobacteriovorax cultured with protist & prey

description: Here, we investigate the growth trajectory and predation dynamics of protists, and HBx micro-predators in co-culture with a common prey, V. parahaemolyticus, in a time-series study in marine water microcosms. The microcosms were established with water samples collected from the Apalachicola Bay in northwest Florida, USA and amended with a suspension of prey bacteria. Samples were taken at a high temporal resolution (3-hour intervals for 5 days) to capture detailed measurements and changes in the growth responses of HBx and protists, using both culture- and molecular-based methods. The protists were counted by qPCR and flow cytometry. The HBx were counted by qPCR and a culture plating method
DOI: 10.26008/1912/bco-dmo.905357.1
title: Measured pH and nutrient data acquired during the pH internal consistency experiment.

description: These data include the measured pHt, total alkalinity, dissolved inorganic carbon, raw pHt absorbance, and calculated pHt of 25 batches of seawater as a function of temperature, salinity, and pCO2. These data were used to evaluate the internal consistency of 120 different possible combinations of CO2 system constants. 

The marine inorganic carbon system can be calculated with two measured parameters due to thermodynamic relationships. However, there are many different parameterizations for the required constants and the most accurate or best is not known. These data were used to evaluate the constants and make recommendations for which constants to use, and how to perform CO2 system calculations.
DOI: 10.26008/1912/bco-dmo.905985.1
title: Near-surface abundance information for phytoplankton and zooplankton in the Mid-Atlantic bight determined from surveys made by the continuous plankton recorder from 2014 to 2017

description: This dataset contains four years of data for the continuous plankton recorder survey program's mid-Atlantic bight transect. This dataset contains relative near-surface abundance information for phytoplankton and zooplankton across a transect crossing the continental shelf from Sandy Hook, New Jersey, USA to the Southeast ending at the shelf break. This survey is organized by the Marine Biological Association (formerly the Sir Alister Hardy Foundation). Sample processing for these four years was funded by the National Science Foundation under award number 1851866.
DOI: 10.26008/1912/bco-dmo.905235.1
title: Raw pH data acquired during the pH internal consistency experiment.

description: These data include the measured pHt, total alkalinity, dissolved inorganic carbon, raw pHt absorbance, and calculated pHt of 25 batches of seawater as a function of temperature, salinity, and pCO2. These data were used to evaluate the internal consistency of 120 different possible combinations of CO2 system constants.

The marine inorganic carbon system can be calculated with two measured parameters due to thermodynamic relationships. However, there are many different parameterizations for the required constants and the most accurate or best is not known. These data were used to evaluate the constants and make recommendations for which constants to use, and how to perform CO2 system calculations.
DOI: 10.26008/1912/bco-dmo.905278.1
title: Total alkalinity and dissolved inorganic carbon data measured during the pH internal consistency experiment.

description: These data include the measured pHt, total alkalinity, dissolved inorganic carbon, raw pHt absorbance, and calculated pHt of 25 batches of seawater as a function of temperature, salinity, and pCO2. These data were used to evaluate the internal consistency of 120 different possible combinations of CO2 system constants. 

The marine inorganic carbon system can be calculated with two measured parameters due to thermodynamic relationships. However, there are many different parameterizations for the required constants and the most accurate or best is not known. These data were used to evaluate the constants and make recommendations for which constants to use, and how to perform CO2 system calculations.
DOI: 10.26008/1912/bco-dmo.821268.2
title: Nitrous oxide (N2O) concentrations and associated physicochemical parameters from R/V Atlantis cruise AT15-61 in Jan-Feb 2010 and R/V Melville cruise MV1104 in Mar-Apr 2011 in the Eastern Tropical South Pacific (ETSP)

description: This dataset includes nitrous oxide (N2O) concentrations and associated physicochemical parameters from the CTD sensor package collected on R/V Atlantis cruise AT15-61 in January-February 2010 and R/V Melville cruise MV1104 in March-April 2011 in the Eastern Tropical South Pacific (ETSP). These data were published as Figure 5 in Santoro et al., 2020 (doi:10.1002/essoar.10503499.1).
DOI: 10.26008/1912/bco-dmo.897484.1
title: Noble gas isotope data from field campaigns sampling groundwater in Umatilla, Oregon during September 2020 and in Tucson, Arizona during November 2021

description: This project developed a novel seawater and groundwater noble gas isotope extraction technique, building on an approach pioneered by Dr. Steve Emerson that involves equilibration of headspace and water followed by vacuum removal and disposal of almost all the water. Corrections are then made for the lost noble gas in the water, using precisely measured mass and volume. The method uses 6-liter stainless steel flasks with Nupro valves for improved leak-tightness. This extraction technique made it possible to measure neon as well as the heavier noble-gas isotopes, all from the same sample, providing a constraint on &quot;excess air&quot; using the neon. Field campaigns sampling groundwater were conducted in Oregon and Arizona. Data from these campaigns are deposited here.
DOI: 10.26008/1912/bco-dmo.903891.1
title: Nitrate (NO3-) and Nitrite (NO2-) d15N and d18O from R/V Atlantis cruise AT15-61 in Jan-Feb 2010 and R/V Melville cruise MV1104 in Mar-Apr 2011 in the Eastern Tropical South Pacific (ETSP)

description: This dataset includes nitrate (NO3-) and nitrite (NO2-) d15N and d18O from R/V Atlantis cruise AT15-61 in January-February 2010 and R/V Melville cruise MV1104 in March-April 2011 in the Eastern Tropical South Pacific (ETSP). Accompanying physiochemical data can be found in a related BCO-DMO dataset, https://www.bco-dmo.org/dataset/821268.
DOI: 10.26008/1912/bco-dmo.904358.1
title: Nitrate plus nitrite isotopic compositions from Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov from December 2016 to January 2017

description: Here we present nitrate plus nitrite isotopic compositions from Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov. This cruise sampled surface and water-column profiles of the summertime Indian Sector of the Southern Ocean, starting in Cape Town, South Africa and ending in Hobart, Australia from December 2016 to January 2017. The data collected focused on potential drivers of the nitrate plus nitrite isotope effect.