DOI: 10.6084/m9.figshare.5929540
title: Drosophila images from predation experiment with the jumping spider salticus scenicus.

description: Experimental Design for Analysis of Phenotypic Selection on <i>Drosophila melanogaster</i> Survival During Episodic Selection from <i>Salticus Scenicus</i> Predation.<br><br>Data collected by Cody Porter in the lab of Ian Dworkin at Michigan State University in 2011.<br><br>Images are organized in folders by whether the flies survived the experiment, or were predated upon by the spiders (dead). I have zipped up the images. Each morphological structure (wings, legs, thorax) are in their own images. For survivors each leg (1st - 3rd) has its own folder.<br><br>***How experiment was performed***<br><br>Fenn Valley (Michigan) wild type population of <i>Drosophila melanogaster</i> will be used in assays (flies aged between 3 and 6 days only).<br><br>24 flies per container (12 males and 12 females) randomly assorted with respect to age.<br> <br>Flies spent at least 24 hours recovering from CO<sub><sup>2</sup></sub> exposure while acclimating in the 24C incubator.<br>Flies that are collected (in vials) must be assorted with respect to sex.<br>*Virgin and non-virgin flies used.<br><br>A.M. Set-up <br><br>Plastic “cages” washed with 70 % ethanol followed by a rinsing with RO H<sub>2</sub>0.<br>1 Spider per cage (begin with adults only and use juveniles as excess flies allow).<br>Acclimate spider in plastic cage with synthetic plant and moist kim wipe for 24 hours in 24c incubator.<br>Introduce flies (that have been prepared and assorted as described above) into “cages” containing spiders using plastic funnel. *Be sure to make note of any dead flies in vial or escapees during this process.<br>Set containers in trays and into 24c incubator and allow assay to run for 24 hours.<br>Add one “cap” of fly food with a single yeast granule per container.<br>*Record everything carefully in notebook.<br>*Label each individual cage with a number that corresponds to the spider present in that cage (from the spider’s vial number).<br><br>A.M. (72 hours later) Take-down<br><br>Place all containers simultaneously in the 4c refrigerator for 30 minutes.<br>After 45 minutes, take 2 cages out of the refrigerator at a time.<br>For each container, count and record the number of survivors and their sex.<br>Place all survivor carcasses (from all containers) into a single Eppendorf vial with ethanol and all dead carcasses (from all containers) into a single, separate Eppendorf vial with ethanol. <br>*If juveniles are used, place carcasses in separate, labeled vials with ethanol, just as with adults.<br><br>Miscellaneous Notes: <br><br>Have everyone remove mite nets prior to placing cages into incubator.<br>Deprive spiders of food (<i>D. melanogaster)</i> for 96 hours prior to assay.<br><br>***How traits were imaged (zoom, scope, etc...)***<br><br>Microscope - Leica M125 with a Leica DFC400 Camera<br><br>- Zoom Drive (1.00X)<br><br>Magnifications used<br>50.0x -- All three legs on left side (5.0)<br>40.0x -- Both wings (4.0)<br>25.0x -- Thorax (2.5)<br><br>-Image Formats<br><br>Captured Format -- 1392x1040, Full Frame HQ<br><br>Live Format -- 1392x1040, Full Frame<br><br>Image Type -- Greyscale<br><br>Iris Manual -- 27% <br><br>***Naming convention for images***<br><br>Salticus scenicus_Survivorship_Sex_Experiment#_Trait_Side(or leg #)<br><br>Example - Ss_S_F_1_W_L<br><br>=Salticus scenicus;survivor;female;Experiment#1;Wing;Left<br><br>**A 'X' next to, or in place of, any piece of information denotes missing information.<br><br>Example - Ss_D_X_2_LX_2<br><br>=Salticus scenicus;dead;sex unknown; Experiment#2;Leg (side of body unknown);leg#2