cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4580752.v1
title: Digestive cell lysosomes as main targets for Ag accumulation and toxicity in marine mussels, <i>Mytilus galloprovincialis</i>, exposed to maltose-stabilised Ag nanoparticles of different sizes

description: Bioavailability and toxicity of maltose-stabilised AgNPs of different sizes (20, 40 and 100 nm) in mussels were compared with bulk and aqueous forms of the metal through a two-tier experimental approach. In the first tier, mussels were exposed for 3 d to a range of concentrations (0.75, 75, 750 μg Ag/l) in the form of Ag20-Mal, Ag40-Mal, Ag100-Mal, bulk Ag and aqueous Ag (as AgNO<sub>3</sub>), as well as to the concentrations of maltose used in the formulation of NPs. Mortality, bioaccumulation, tissue and cell distribution and lysosomal responses were investigated. In the second tier, mussels were exposed for 21 d to Ag20-Mal, Ag100-Mal, bulk Ag and aqueous Ag at the lowest effective concentration selected after <i>Tier 1</i> (0.75 μg Ag/l), biomarkers and toxicopathic effects were investigated. Aqueous Ag was lethal within 3 d at 75 μg Ag/l; Ag NPs or bulk Ag did not produce significant mortality at 750 μg Ag/l. Ag accumulation was limited and metallothionein gene transcription was not regulated although metal accumulation occurred in digestive, brown and stomach epithelial cells and in gut lumen after exposure to AgNPs and aqueous Ag starting at low concentrations after 1 d. Electrondense particles (&lt;10 nm) in lysosomes and residual bodies after exposure to AgNPs contained Ag and S (X-ray). Intralysosomal metal accumulation and lysosomal membrane destabilisation were enhanced after exposure to all the forms of Ag and more marked after exposure to Ag20-Mal than to larger NPs. 21 d exposure to AgNPs provoked digestive cell loss and loss of digestive gland integrity, resulting in atrophy-necrosis in digestive alveoli and oedema/hyperplasia in gills (Ag NP), vacuolisation in digestive cells (aqueous Ag) and haemocyte infiltration of connective tissue (all treatments). Intralysosomal metal accumulation, lysosomal responses and toxicopathic effects are enhanced at decreasing sizes and appear to be caused by Ag<sup>+ </sup>ions released from NPs, although the metal was not substantially accumulated.
DOI: 10.6084/m9.figshare.4580695
title: Zircon Lu–Hf isotopic compositions. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S2: Zircon Lu-Hf isotope-4 samples
DOI: 10.6084/m9.figshare.4580698
title: U–Pb geochronological data. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S1: Dating data of the pluton-7 settings
DOI: 10.6084/m9.figshare.4580701.v2
title: <sup>40</sup>Ar/<sup>39</sup>Ar ages. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S4: Summary of Early Mesozoic metamorphic, deformation and granite-related Nb–Ta–W–Sn.
DOI: 10.6084/m9.figshare.4580698.v2
title: U–Pb geochronological data. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S1: Dating data of the pluton-7 settings
DOI: 10.6084/m9.figshare.4580701.v1
title: <sup>40</sup>Ar/<sup>39</sup>Ar ages. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S4: Summary of Early Mesozoic metamorphic, deformation and granite-related Nb–Ta–W–Sn.
DOI: 10.6084/m9.figshare.4580704.v2
title: Major and trace element data. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S3: Major REEs Trace of 15 samples.
DOI: 10.6084/m9.figshare.4580704.v1
title: Major and trace element data. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S3: Major REEs Trace of 15 samples.
DOI: 10.6084/m9.figshare.4580695.v1
title: Zircon Lu–Hf isotopic compositions. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S2: Zircon Lu-Hf isotope-4 samples
DOI: 10.6084/m9.figshare.4580698.v1
title: U–Pb geochronological data. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S1: Dating data of the pluton-7 settings
DOI: 10.6084/m9.figshare.4580593
title: Figure 8

description: Figure 8
DOI: 10.6084/m9.figshare.4580593.v1
title: Figure 8

description: Figure 8
DOI: 10.6084/m9.figshare.4580587
title: Figure 6

description: Figure 6
DOI: 10.6084/m9.figshare.4580590
title: Figure 7

description: Figure 7
DOI: 10.6084/m9.figshare.4580590.v1
title: Figure 7

description: Figure 7
DOI: 10.6084/m9.figshare.4580575
title: Figure 4

description: Figure 4
DOI: 10.6084/m9.figshare.4580581
title: Figure 5

description: Figure 5
DOI: 10.6084/m9.figshare.4580569
title: Figure 2

description: Figure 2
DOI: 10.6084/m9.figshare.4580572
title: Figure 3

description: Figure 3
DOI: 10.6084/m9.figshare.4580581.v1
title: Figure 5

description: Figure 5
DOI: 10.6084/m9.figshare.4580569.v1
title: Figure 2

description: Figure 2
DOI: 10.6084/m9.figshare.4580563
title: cafe recapitulatif

description: Ce projet contient des données de comptage d'une expérience réalisée avec acinetobacter baylyi, ainsi que les métadonnées dans un autre fichier csv, et les valeurs de DO associées dans un troisième fichier csv.
DOI: 10.6084/m9.figshare.4580575.v1
title: Figure 4

description: Figure 4
DOI: 10.6084/m9.figshare.4580563.v1
title: cafe recapitulatif

description: Ce projet contient des données de comptage d'une expérience réalisée avec acinetobacter baylyi, ainsi que les métadonnées dans un autre fichier csv, et les valeurs de DO associées dans un troisième fichier csv.
DOI: 10.6084/m9.figshare.4580572.v1
title: Figure 3

description: Figure 3
DOI: 10.6084/m9.figshare.4580518
title: The role of 5-arylalkylamino- and 5-piperazino- moieties on the 7-aminopyrazolo[4,3-<i>d</i>]pyrimidine core in affecting adenosine A<sub>1</sub> and A<sub>2A</sub> receptor affinity and selectivity profiles

description: New 7-amino-2-phenylpyrazolo[4,3-<i>d</i>]pyrimidine derivatives, substituted at the 5-position with aryl(alkyl)amino- and 4-substituted-piperazin-1-yl- moieties, were synthesized with the aim of targeting human (h) adenosine A<sub>1</sub> and/or A<sub>2A</sub> receptor subtypes. On the whole, the novel derivatives <b>1</b>–<b>24</b> shared scarce or no affinities for the off-target hA<sub>2B</sub> and hA<sub>3</sub> ARs. The 5-(4-hydroxyphenethylamino)- derivative <b>12</b> showed both good affinity (<i>K<sub>i</sub></i> = 150 nM) and the best selectivity for the hA<sub>2A</sub> AR while the 5-benzylamino-substituted <b>5</b> displayed the best combined hA<sub>2A</sub> (<i>K<sub>i</sub></i> = 123 nM) and A<sub>1</sub> AR affinity (<i>K<sub>i</sub></i> = 25 nM). The 5-phenethylamino moiety (compound <b>6</b>) achieved nanomolar affinity (<i>K<sub>i</sub></i> = 11 nM) and good selectivity for the hA<sub>1</sub> AR. The 5-(N<sup>4</sup>-substituted-piperazin-1-yl) derivatives <b>15</b>–<b>24</b> bind the hA<sub>1</sub> AR subtype with affinities falling in the high nanomolar range. A structure-based molecular modeling study was conducted to rationalize the experimental binding data from a molecular point of view using both molecular docking studies and Interaction Energy Fingerprints (IEFs) analysis.
DOI: 10.6084/m9.figshare.4580518.v1
title: The role of 5-arylalkylamino- and 5-piperazino- moieties on the 7-aminopyrazolo[4,3-<i>d</i>]pyrimidine core in affecting adenosine A<sub>1</sub> and A<sub>2A</sub> receptor affinity and selectivity profiles

description: New 7-amino-2-phenylpyrazolo[4,3-<i>d</i>]pyrimidine derivatives, substituted at the 5-position with aryl(alkyl)amino- and 4-substituted-piperazin-1-yl- moieties, were synthesized with the aim of targeting human (h) adenosine A<sub>1</sub> and/or A<sub>2A</sub> receptor subtypes. On the whole, the novel derivatives <b>1</b>–<b>24</b> shared scarce or no affinities for the off-target hA<sub>2B</sub> and hA<sub>3</sub> ARs. The 5-(4-hydroxyphenethylamino)- derivative <b>12</b> showed both good affinity (<i>K<sub>i</sub></i> = 150 nM) and the best selectivity for the hA<sub>2A</sub> AR while the 5-benzylamino-substituted <b>5</b> displayed the best combined hA<sub>2A</sub> (<i>K<sub>i</sub></i> = 123 nM) and A<sub>1</sub> AR affinity (<i>K<sub>i</sub></i> = 25 nM). The 5-phenethylamino moiety (compound <b>6</b>) achieved nanomolar affinity (<i>K<sub>i</sub></i> = 11 nM) and good selectivity for the hA<sub>1</sub> AR. The 5-(N<sup>4</sup>-substituted-piperazin-1-yl) derivatives <b>15</b>–<b>24</b> bind the hA<sub>1</sub> AR subtype with affinities falling in the high nanomolar range. A structure-based molecular modeling study was conducted to rationalize the experimental binding data from a molecular point of view using both molecular docking studies and Interaction Energy Fingerprints (IEFs) analysis.
DOI: 10.6084/m9.figshare.4579792
title: Figure 1

description: Figure 1 data
DOI: 10.6084/m9.figshare.4579690
title: Daily Integrated Eddy Data

description: Daily sums for NEE, CH4 and mean data from meteorological variables. Also, included are flux rates converted to mass units and cumulative fluxes used to create annual greenhouse gas budgets.
DOI: 10.6084/m9.figshare.4579681
title: Ancient environmental DNA of eukaryotes, organohalogene pesticides, DNA extraction kits and laboratory issues

description: Supporting files for the paper "Proper experimental design requires randomization/balancing of molecular ecology experiments".
DOI: 10.6084/m9.figshare.4579792.v1
title: Figure 1

description: Figure 1 data
DOI: 10.6084/m9.figshare.4579654
title: Quilt plot functions

description: Functions for Quilt plot
DOI: 10.6084/m9.figshare.4579690.v1
title: Daily Integrated Eddy Data

description: Daily sums for NEE, CH4 and mean data from meteorological variables. Also, included are flux rates converted to mass units and cumulative fluxes used to create annual greenhouse gas budgets.
DOI: 10.6084/m9.figshare.4579681.v1
title: Ancient environmental DNA of eukaryotes, organohalogene pesticides, DNA extraction kits and laboratory issues

description: Supporting files for the paper "Proper experimental design requires randomization/balancing of molecular ecology experiments".
DOI: 10.6084/m9.figshare.4579654.v1
title: Quilt plot functions

description: Functions for Quilt plot
DOI: 10.6084/m9.figshare.4579543
title: Effect of porcine placental extract on the mild menopausal symptoms of climacteric women

description: <b>Objectives:</b> This study assessed the effects of oral porcine placental extract (PPE) on the mild menopausal symptoms of climacteric women. <b>Methods:</b> In this 12-week, multicenter, randomized, double-blind, placebo-controlled, parallel-group study, 50 climacteric Japanese women were randomized 1 : 1 to oral PPE (300 mg/day) or placebo. Menopausal symptoms were evaluated by using the Simplified Menopausal Index (SMI), as were serum estradiol (E2) and follicle stimulating hormone (FSH) levels. Blood biochemical and cellular and urinary tests were done to evaluate safety aspects of repeated oral administration of PPE. <b>Results:</b> The total SMI score of the PPE group was significantly more improved after 12 weeks than that of the placebo group (<i>p</i> = 0.031). This score and three subscores (vasomotor, psychological, and somatic symptoms) were significantly improved at 8 and/or 12 weeks compared with the initial values in the PPE group (<i>p</i> <b>Conclusions:</b> Oral PPE at 300 mg/day improved the mild menopausal symptoms of climacteric women. Since oral PPE did not improve serum E2 and FSH levels, PPE is thought not to ameliorate hormonal balance itself but to improve subjective feelings of climacteric women.
DOI: 10.6084/m9.figshare.4579474
title: Data.rar

description: Here we include the data generated by the simulations of the model presented in the research paper "A Detailed Heterogeneous Agent Model for a Single Asset Financial Market With Trading Via an Order Book" to be published in PLOS ONE.<br>Price time series as well as power law fit results and hypothesis tests results are included.<br>There is a text file inside each folder with a detailed description of the nature of the data.
DOI: 10.6084/m9.figshare.4579540
title: Tolerance for Audiovisual Onset Asynchrony

description: Please read the ReadMe.doc file for description
DOI: 10.6084/m9.figshare.4579474.v1
title: Data.rar

description: Here we include the data generated by the simulations of the model presented in the research paper "A Detailed Heterogeneous Agent Model for a Single Asset Financial Market With Trading Via an Order Book" to be published in PLOS ONE.<br>Price time series as well as power law fit results and hypothesis tests results are included.<br>There is a text file inside each folder with a detailed description of the nature of the data.
DOI: 10.6084/m9.figshare.4579540.v1
title: Tolerance for Audiovisual Onset Asynchrony

description: Please read the ReadMe.doc file for description
DOI: 10.6084/m9.figshare.4579543.v1
title: Effect of porcine placental extract on the mild menopausal symptoms of climacteric women

description: <b>Objectives:</b> This study assessed the effects of oral porcine placental extract (PPE) on the mild menopausal symptoms of climacteric women. <b>Methods:</b> In this 12-week, multicenter, randomized, double-blind, placebo-controlled, parallel-group study, 50 climacteric Japanese women were randomized 1 : 1 to oral PPE (300 mg/day) or placebo. Menopausal symptoms were evaluated by using the Simplified Menopausal Index (SMI), as were serum estradiol (E2) and follicle stimulating hormone (FSH) levels. Blood biochemical and cellular and urinary tests were done to evaluate safety aspects of repeated oral administration of PPE. <b>Results:</b> The total SMI score of the PPE group was significantly more improved after 12 weeks than that of the placebo group (<i>p</i> = 0.031). This score and three subscores (vasomotor, psychological, and somatic symptoms) were significantly improved at 8 and/or 12 weeks compared with the initial values in the PPE group (<i>p</i> <b>Conclusions:</b> Oral PPE at 300 mg/day improved the mild menopausal symptoms of climacteric women. Since oral PPE did not improve serum E2 and FSH levels, PPE is thought not to ameliorate hormonal balance itself but to improve subjective feelings of climacteric women.
DOI: 10.6084/m9.figshare.4579348
title: Supplementary table 3: Pairwise Fst values (below diagonal) and corresponding p-values (above diagonal) calculated using13 microsatellites genotyped in 45 individuals from RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity

description: The field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (<i>Pecten maximus</i>) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher <i>F</i><sub>st</sub> values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying both population structure and phenotypic plasticity in natural populations.
DOI: 10.6084/m9.figshare.4579345
title: Supplementary table 2: Pairwise Fst values (below diagonal) and corresponding p-values (above diagonal) calculated using (a) 13 microsatellites genotyped in 180 individuals and (b) 10,539 SNPs genotyped in 45 individuals from RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity

description: The field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (<i>Pecten maximus</i>) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher <i>F</i><sub>st</sub> values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying both population structure and phenotypic plasticity in natural populations.
DOI: 10.6084/m9.figshare.4579348.v1
title: Supplementary table 3: Pairwise Fst values (below diagonal) and corresponding p-values (above diagonal) calculated using13 microsatellites genotyped in 45 individuals from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: The field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (<i>Pecten maximus</i>) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher <i>F</i><sub>st</sub> values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying both population structure and phenotypic plasticity in natural populations.
DOI: 10.6084/m9.figshare.4579345.v1
title: Supplementary table 2: Pairwise Fst values (below diagonal) and corresponding p-values (above diagonal) calculated using (a) 13 microsatellites genotyped in 180 individuals and (b) 10,539 SNPs genotyped in 45 individuals from ddRAD sequencing resolves fine-scale population structure in a benthic invertebrate with implications for understanding phenotypic plasticity

description: The field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (<i>Pecten maximus</i>) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher <i>F</i><sub>st</sub> values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying both population structure and phenotypic plasticity in natural populations.