DOI: 10.5061/dryad.zcrjdfncd
title: Mating systems and predictors of relative reproductive success in a Cutthroat Trout subspecies of conservation concern

description: Mating systems and patterns of reproductive success in fishes play an
 important role in ecology and evolution. While information on the
 reproductive ecology of many anadromous salmonids (Oncorhynchus spp.) is
 well-detailed, there is less information for non-anadromous species
 including the Yellowstone Cutthroat Trout (O. clarkii bouvieri), a
 subspecies of recreational angling importance and conservation concern.
 Using data from a parentage-based tagging study, we described the genetic
 mating system of a migratory population of Yellowstone Cutthroat Trout,
 tested for evidence of sexual selection, and identified predictors of
 mating and reproductive success. The standardized variance in mating
 success (i.e., opportunity for sexual selection) was significantly greater
 for males relative to females, and while the relationship between mating
 success and reproductive success (i.e., Bateman gradient) was
 significantly positive for both sexes, a greater proportion of
 reproductive success was explained by mating success for males (r2 = 0.80)
 than females (r2 = 0.59). Overall, the population displayed a
 polygynandrous mating system, whereby both sexes experienced variation in
 mating success due to multiple mating, and sexual selection was variable
 across sexes. Tests for evidence of sexual selection indicated the
 interaction between mating success and total length best predicted
 relative reproductive success. We failed to detect a signal of inbreeding
 avoidance among breeding adults, but the group of parents that produced
 progeny were on average slightly less related than adults that did not
 produce progeny. Lastly, we estimated the effective number of breeders
 (Nb) and effective population size (Ne) and identified while Nb was lower
 than Ne, both are sufficiently high to suggest Yellowstone Cutthroat Trout
 in Burns Creek represent a genetically stable and diverse population.
description: The genetic data set provided was generated via sampling of
 Yellowstone Cutthroat Trout at a weir on a spawning tributary (Burns
 Creek) of the South Fork Snake River, Idaho, USA. Each migrating adult was
 sampled for a genetic sample and the length and date of sampling was
 recorded for each individual. Juveniles emigrating from the same system
 later the same year were also sampled for genetics. In the lab, DNA was
 extracted and each individual was amplified at a panel of 134 single
 nucleotide polymorphisms (SNPs) using a Genotyping-in-Thousands (GT-seq)
 protocol. Parentage analysis was performed using the
 algorithms implemented in SNPPIT, specifically juveniles were assigned to
 parent-pairs. We created reproductive profiles for each adult including
 the number of offspring assigned, number of mates. Analysis of genetic
 mating systems involved summarizing the means and variances of
 reproductive success (offspring assigned) and mating success (number of
 mates) for each sex. We compared phenotypic traits of fish that did and
 did not mate to test for evidence of sexual selection.
description: Please see the attached README file for a detailed description of
 the data included in the dataset and how data were analyzed