DOI: 10.5061/dryad.v41ns1sb1
title: Data from: Dual pH-and thermoresponsive nano-objects with tunable morphologies and dye encapsulation prepared via aqueous emulsion PISA

description: Poly(triethylene glycol methyl ether methacrylate)-b-poly
 [2-(diisopropylamino)ethyl methacrylate] (PTEGMAb-PDPA) nano-objects,
 synthesized via polymerization-induced self-assembly (PISA), exhibit dual
 thermo-and pH-responsive behavior. The thermoresponsiveness originates
 from the lower critical solution temperature (LCST) of the PTEGMA block,
 while the pH-responsiveness arises from protonation of the PDPA block
 under acidic conditions. The hydrophilic PTEGMA block (degree of
 polymerization, DP = 120) was first synthesized to serve as a macro-chain
 transfer agent. This was followed by RAFT-mediated PISA emulsion
 polymerization of the pH-sensitive PDPA block (DP = 35-265) in aqueous
 solution at a solid content of 10 wt%. During polymerization, the block
 copolymers self-assembled in situ into diverse and tunable nanostructures,
 including micelles, branched worms, "jellyfish", and
 thick-walled vesicles. These nano-objects were thoroughly characterized
 using cryo-transmission electron microscopy (cryo-TEM), dynamic light
 scattering (DLS), and small-angle X-ray scattering (SAXS). The in situ
 drug encapsulation capability of these dual-responsive nanoformulations
 was demonstrated using Nile Red (NR), a hydrophobic fluorescent model
 compound. Specifically, thick-walled vesicles (PTEGMA 120 -b-PDPA 265 )
 successfully encapsulated NR during RAFT-PISA synthesis and released it
 under acidic conditions and/or at mildly elevated temperatures. These
 results highlight the potential of tailorable PTEGMA-b-PDPA nano-objects
 as "smart" nanocarriers for targeted and stimuli-responsive drug
 delivery applications.
description: # Data from: Dual pH-and thermoresponsive nano-objects with tunable
 morphologies and dye encapsulation prepared via aqueous emulsion PISA
 Dataset DOI:
 [10.5061/dryad.v41ns1sb1](https://doi.org/10.5061/dryad.v41ns1sb1) ##
 Description of the data and file structure This folder contains raw DLS,
 cryo-TEM, NMR, SEC, and SAXS data forthe article titled "Dual pH- and
 Thermoresponsive Nano-Objects with Tunable Morphologies and Dye
 Encapsulation Prepared via Aqueous Emulsion PISA." ### Files and
 variables #### File: PTEGMA120-PDPA35_SAXS.txt **Description:** SAXS raw
 data for the sample PTEGMA120-PDPA35 ##### Variables * Q/nm ^-1^ *
 Intensity/a.u. #### File: PTEGMA120-PDPA75_SAXS.txt **Description:** SAXS
 raw data for the sample PTEGMA120-PDPA75 ##### Variables * Q/nm ^-1^ *
 Intensity/a.u. #### File: PTEGMA120-PDPA265_SAXS.txt **Description:** SAXS
 raw data for the sample PTEGMA120-PDPA265 ##### Variables * Q/nm ^-1^ *
 Intensity/a.u. #### File: PTEGMA120-PDPA265-NR_65C_SAXS.txt
 **Description:** SAXS raw data for the sample PTEGMA120-PDPA265-NR at 65
 °C ##### Variables * Q/nm ^-1^ * Intensity/a.u. #### File:
 PTEGMA120-PDPA135_SAXS.txt **Description:** SAXS raw data for the sample
 PTEGMA120-PDPA135 ##### Variables * Q/nm ^-1^ * Intensity/a.u. #### File:
 PTEGMA120-PDPA265-NR_25C_SAXS.txt **Description:** SAXS raw data for the
 sample PTEGMA120-PDPA265-NR at 25 °C ##### Variables * Q/nm ^-1^ *
 Intensity/a.u. #### File: NMR_Figure_S3.txt **Description:** proton NMR
 raw data for the sample PTEGMA120-b-PDPA265 ##### Variables * Chemical
 shift / ppm * Frequency / Hz #### File: NMR_Figure_S2.txt
 **Description:** proton NMR raw data for the sample PTEGMA ##### Variables
 * Chemical shift / ppm * Frequency / Hz #### File: NMR_Figure_S1.txt
 **Description:** proton NMR raw data for the sample CETPA ##### Variables
 * Chemical shift / ppm * Frequency / Hz #### File: SEC_-_Figure_S4.opju
 **Description:** Origin project containing raw SEC data for this
 manuscript #### File: cryoTEM_Fig.3_(B).tif **Description:** raw cryo-TEM
 picture for PTEGMA120-PDPA265-NR at 65C #### File: cryoTEM_Fig.3_(C).tif
 **Description:** raw cryo-TEM picture for PTEGMA120-PDPA265-NR at pH 5.5
 #### File: cryoTEM_Figure_S6.tif **Description:** raw cryo-TEM image of
 PTEGMA125-*b*-PDPA135 `File: cryoTEM_Fig._3_(A).tif` **Description:** raw
 cryo-TEM picture for PTEGMA120-PDPA265-NR at 25 °C #### File:
 cryoTEM_Fig.1_(G).tif **Description:** PTEGMA120-PDPA265 #### File:
 cryoTEM_Fig.1_(C).tif **Description:** PTEGMA120-PDPA75 #### File:
 cryoTEM_Fig.1_(E).tif **Description:** PTEGMA120-PDPA135 #### File:
 cryoTEM_Fig.1_(A).tif **Description:** PTEGMA120-PDPA35 #### File:
 DLS_Figure_2_(B).opju **Description:** Origin project containing raw DLS
 data for temperature dependence of the *D*~H~ of PTEGMA~120~-*b*-PDPA~n~
 nano-objects #### File: DLS_Figure2.(A).opju **Description:** Origin
 project containing raw DLS data for temperature dependence of
 pH-responsive behavior #### File: DLS_Figure_S7.opju
 **Description:** Origin project containing raw DLS data for temperature
 dependence of the *D*~H~ of PTEGMA~120~-*b*-PDPA~265~-NR Vs #### File:
 DLS_Figure_S5.opju **Description:** Origin project containing raw DLS data
 for particle size distributions obtained for PTEGMA~120~-*b*-PDPA~n~ (n =
 35, 75, 135, and 265) #### File: DLS_Figure_3_(D).opju
 **Description:** Origin project containing raw DLS data for
 PTEGMA~120~-*b*-PDPA~265~-NR at 25 °C, 40.1 °C and under acidic conditions
 (pH = 5.5) ## Code/software SAXS and NMR data can be opened using Notepad.
 DLS and SEC data can be opened using Origin. cryo-TEM pictures can be
 opened using IrfanView. ## Access information