DOI: 10.5061/dryad.zw3r228dq
title: Local adaptation in trait-mediated trophic cascades dataset

description: Predator-induced changes in prey foraging can indirectly influence
 community dynamics by increasing the abundance of basal resources via a
 trait-mediated trophic cascade. The strength of these cascades may be
 altered by eco-evolutionary relationships between predators and prey, but
 the role of basal resources has received limited attention. We
 hypothesized that the strength of trait-mediated trophic cascades may be
 shaped by selection from trophic levels above and below prey. Our field
 and laboratory experiments utilized snails (Nucella
 lapillus) from two regions in the Gulf of Maine (GoM) that vary
 substantially in basal resource (e.g., barnacles, mussels) availability
 and invasion history with the predatory green crab, Carcinus
 maenas. Nucella from both regions were provided with
 mussels as a basal resource and exposed to the presence or absence of
 green crab risk cues. In both experiments, Nucella from
 the northern GoM, where basal resources are scarce, displayed weaker
 responses to risk cues than southern Nucella. In the lab,
 however, northern Nucella foraged more than southern snails under
 risk, but foraging rates among the two regions did not differ without
 risk. Our results suggest that adaptation to basal resource availability
 may contribute to geographic variation in the strength of trait-mediated
 trophic cascades.
description: # **Local adaptation in trait-mediated trophic cascades dataset**
 ### **Description of the
 data and file structure** The dataset contains seven Excel spreadsheets
 which encompass all of the data used in statistical analyses mentioned in
 this manuscript. All data are presented in tabular form. Spreadsheet
 descriptions and column definitions are provided below.
 **FieldExperiment_ConsumptionData** - Contains the data used in analyses
 of mussel consumption per capita during the field experiment.
 FieldExperiment_ConsumptionData: *Column names and definitions* *
 **Row_Id** – Numbers each row in the dataset excluding the headers. *
 **Region** – Denotes the region of the Gulf of Maine that *Nucella* were
 collected from and outplanted to for the field experiment. * **Site** –
 Indicates the site (population) that *Nucella* were collected from and
 outplanted to for the field experiment. * **Treatment** – Indicates
 whether *Nucella* received green crab risk cue treatment or control
 treatment (absence of risk cue) during the field experiment. *
 **ReplicateChamber** - Denotes the specific stimulus chamber that
 *Nucella* were placed in for a given Site x Treatment combination. For
 instance, “NHT.C1” indicates that the chamber placed at Nahant, received
 green crab risk cue treatment, and was #1 of four chambers within this
 Site x Treatment group. * **ConsumptionPerCapita** **–** The number of
 mussels consumed per capita in each replicate chamber. This metric was
 calculated by dividing the total number of mussels consumed in a given
 chamber by the average number of *Nucella* in the replicate chamber (4/#
 survived).  **FieldExperiment_GrowthData** - Contains the data used in
 analyses of growth in shell length, shell mass, and tissue mass during the
 field experiment. FieldExperiment_GrowthData: *Column names and
 definitions* * **Row_Id** – Numbers each row in the dataset excluding the
 headers. * **Region** – Denotes the region of the Gulf of Maine that
 *Nucella* were collected from and outplanted to for the field experiment.
 * **Site** – Represents the site that *Nucella* were collected from and
 outplanted to for the field experiment. * **Treatment** – Indicates
 whether *Nucella* received green crab risk cue treatment or control
 treatment (absence of risk cue) during the field experiment. *
 **Replicate** - Denotes the specific stimulus chamber that *Nucella* were
 placed in for a given Site x Treatment combination. * **LengthGrowth**
 **–** Represents the amount of shell length growth (mm) exhibited by a
 given snail over the course of the field experiment. * **ShellGrowth**
 **–** Represents the amount of shell mass growth (g) exhibited by a given
 snail over the course of the field experiment. * **TissueGrowth** **–**
 Represents the amount of tissue mass growth (g) exhibited by a given snail
 over the course of the field experiment.
 **FieldExperiment_TemperatureData** - Contains the data used in the
 analysis of water temperature across the two regions.
 FieldExperiment_TemperatureData: *Column names and definitions* *
 **Row_Id** – Numbers each row in the dataset excluding the headers. *
 **Region** – Denotes each region in the Gulf of Maine (northern or
 southern) where seawater temperature (°C) was recorded during the field
 experiment. * **Site** - The site where seawater temperature (°C) was
 recorded during the field experiment. * **Temperature** – Refers to the
 seawater temperature (°C) recorded at each high tide (± 10 minutes) during
 the field experiment. * **Date** – The date that a given seawater
 temperature (°C) was recorded on. **FieldExperiment_TempTraitData** –
 Contains the data used in the analyses of mussel consumption and tissue
 mass growth as a function of water temperature.
 FieldExperiment_TempTraitData: *Column names and definitions* * **Region**
 – Denotes the region of the Gulf of Maine that *Nucella* were collected
 from and outplanted to for the field experiment. * **Treatment** –
 Indicates whether *Nucella* received green crab risk cue treatment or
 control treatment (absence of risk cue) during the field experiment. *
 **Site** - The site (population) that *Nucella* were collected from and
 outplanted to for the field experiment. * **SiteNumber** – Numerically
 orders sites used in the field experiment from south to north (1-8
 respectively) to make the dataset easier to arrange and visualize as a
 function of latitude. * **TissueGrowth** – The least-squares mean for
 tissue growth (g) within a given Site by Treatment combination. *
 **SE.TissueGrowth** – The standard error for tissue mass growth (g) within
 a given Site by Treatment combination. * **Consumption** – The
 least-squares mean for mussel consumption per capita within a given Site
 by Treatment combination. * **SE.Consumption** – The standard error for
 mussel consumption per capita within a given Site by Treatment
 combination. **LabExperiment_ConsumptionData** - Contains the data used in
 analyses of mussel consumption per capita during the lab experiment.
 LabExperiment_ConsumptionData: *Column names and definitions* * **Row_Id**
 – Numbers each row in the dataset excluding the headers. * **Region** –
 Denotes the region of the Gulf of Maine that *Nucella* were collected from
 for the lab experiment. * **Site** – Indicates the site (population) that
 *Nucella* were collected from for the lab experiment. * **Treatment** –
 Indicates whether *Nucella* received green crab risk cue treatment or
 control treatment (absence of risk cue) during the lab experiment. *
 **ReplicateChamber** - Denotes the specific stimulus chamber that
 *Nucella* were placed in for a given Site x Treatment combination. *
 **ConsumptionPerCapita** **–** The number of mussels consumed per capita
 in each replicate chamber. This metric was calculated by dividing the
 total number of mussels consumed by the average number of *Nucella* in the
 replicate chamber (4/# survived). **LabExperiment_GrowthData** - Contains
 the data used in analyses of growth in shell length, shell mass, and
 tissue mass during the lab experiment. LabExperiment_GrowthData: *Column
 names and definitions* * **Row_Id** – Numbers each row in the dataset
 excluding the headers. * **Region** – Denotes the region of the Gulf of
 Maine that *Nucella* were collected from for the lab experiment. *
 **Site** – Represents the site (population) that *Nucella* were collected
 from for the lab experiment. * **Treatment** – Indicates whether *Nucella*
 received green crab risk cue treatment or the control treatment (absence
 of risk cue) during the lab experiment. * **Replicate** - Denotes the
 specific stimulus chamber that *Nucella* were placed in for a given Site
 (population) x Treatment combination. * **LengthGrowth** **–** Represents
 the amount of shell length growth (mm) exhibited by a given snail over the
 course of the lab experiment. * **ShellGrowth** **–** Represents the
 amount of shell mass growth (g) exhibited by a given snail over the course
 of the lab experiment. * **TissueGrowth** **–** Represents the amount of
 tissue mass growth (g) exhibited by a given snail over the course of the
 lab experiment. **LabExperiment_BehaviorData** - Contains the data used in
 analyses of refuge habitat use during the lab experiment.
 LabExperiment_BehaviorData: *Column names and definitions* * **Row_Id** –
 Numbers each row in the dataset excluding the headers. * **Region** –
 Denotes the region of the Gulf of Maine (northern or southern) that
 *Nucella* were collected from for the lab experiment. * **Site** –
 Represents the site (population) that *Nucella* were collected from for
 the lab experiment. * **Treatment** – Indicates whether *Nucella* received
 green crab risk cue treatment or control treatment (absence of risk cue)
 during the lab experiment. * **Replicate** - Denotes the specific stimulus
 chamber that *Nucella* were placed in for each Site (population) x
 Treatment combination. * **Week** – Represents the week (numbered 1-6)
 that behavior measurements were recorded during over the course of the lab
 experiment. * **Behavior** – Mean refuge use (1 = all snails using refuge,
 0 = none in refuge) in a given stimulus chamber at a given point in time
 (recorded every 3 days).   **Sharing/Access information** N/A
 **Code/Software** All statistical analyses were conducted in JMP 15 Pro