DOI: 10.5061/dryad.k7t20
title: Data from: A combination of sexual and ecological divergence contributes to rearrangement spread during initial stages of speciation

description: Chromosomal rearrangements between sympatric species often contain
 multiple loci contributing to assortative mating, local adaptation, and
 hybrid sterility. When and how these associations arise during the process
 of speciation remains a subject of debate. Here, we address the relative
 roles of local adaptation and assortative mating on the dynamics of
 rearrangement evolution by studying how a rearrangement co-varies with
 sexual and ecological trait divergence within a species. Previously, a
 chromosomal rearrangement that suppresses recombination on the Z (sex)
 chromosome was identified in European corn borer moths (Ostrinia
 nubilalis). We further characterize this recombination suppressor and
 explore its association with variation in sex pheromone communication and
 seasonal ecological adaptation in pairs of populations that are divergent
 in one or both of these characteristics. Direct estimates of recombination
 suppression in pedigree mapping families indicated that more than 39% of
 the Z chromosome (encompassing up to ~10 megabases and ~ 300 genes)
 resides within a non-recombining unit, including pheromone olfactory
 receptor (OR) genes and a major quantitative trait locus (QTL) that
 contributes to ecotype differences (Pdd). Combining direct and indirect
 estimates of recombination suppression, we found that the rearrangement
 was occasionally present between sexually isolated strains (E versus Z)
 and between divergent ecotypes (univoltine versus bivoltine). However, it
 was only consistently present when populations differed in both sexual and
 ecological traits. Our results suggest that independent of the forces that
 drove the initial establishment of the rearrangement, a combination of
 sexual and ecological divergence is required for rearrangement spread
 during speciation.
description: longestReadsMFTMale and female reproductive tissues de novo transcriptome. Longest transcript for each component selected.longestReadsRCLFemale head tissue de novo transcriptome. Longest transcript for each component selected.longestReadsDipDiapausing larval tissue de novo transcriptome. Longest transcript for each component selected.longestReadsMHPMale hairpencil tissues de novo transcriptome. Longest transcript for each component selected.RS_ampliconsFST values for Rock Springs E and Z using the amplicon reference.Land_ampliconsFST values for Landisville E and Z using the amplicon reference.RS_transcriptsFST values for Rock Springs E and Z using the Z chromosome transcript reference.Land_transcriptsFST values for Landisville E and Z using the Z chromosome transcript reference.Transcripts_Bombyx_LocationsSummary file of prediction locations of transcripts on the Z chromosome using blastn against Bombyx mori.All_BM_ORF_MCRP_dup_rem7.txtPIXY_RS_ampliconsPIXY statistic for Rock Springs E and Z using amplicon reference.PIXY_LandEZ_ampliconsPIXY statistic for Landisville E and Z using amplicon reference.Amplicon_Sequence_Jan2017Amplicon mapping markers reference sequencesLink_Sequence_Jan2017.txtMale_Counts_2016Male reproductive tissue raw transcript counts for 6 E and 6 Z RNA-seq libraries.