cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5360416.v2
title: Generation of genome-modified <i>Drosophila</i> cell lines using SwAP

description: The ease of generating genetically modified animals and cell lines has been markedly increased by the recent development of the versatile CRISPR/Cas9 tool. However, while the isolation of isogenic cell populations is usually straightforward for mammalian cell lines, the generation of clonal <i>Drosophila</i> cell lines has remained a longstanding challenge, hampered by the difficulty of getting <i>Drosophila</i> cells to grow at low densities. Here, we describe a highly efficient workflow to generate clonal Cas9-engineered <i>Drosophila</i> cell lines using a combination of cell pools, limiting dilution in conditioned medium and PCR with allele-specific primers, enabling the efficient selection of a clonal cell line with a suitable mutation profile. We validate the protocol by documenting the isolation, selection and verification of eight independently Cas9-edited <i>armadillo</i> mutant <i>Drosophila</i> cell lines. Our method provides a powerful and simple workflow that improves the utility of <i>Drosophila</i> cells for genetic studies with CRISPR/Cas9.
DOI: 10.6084/m9.figshare.5361007.v1
title: The complexity of high-frequency electric fields impairs jamming avoidance: a potential trade-off in electric sensing

description: Accompanying data to "The complexity of high-frequency electric fields impairs jamming avoidance: a potential trade-off in electric sensing" (submitted)
DOI: 10.6084/m9.figshare.5358562.v1
title: Complete data for 96 countries

description: Contains data on UV radiation, complex cognitive ability, genetic distance from South Africa and other variables.<br><br>
DOI: 10.6084/m9.figshare.5360416.v1
title: Generation of genome-modified Drosophila cell lines using SwAP

description: The ease of generating genetically modified animals and cell lines has been markedly increased by the recent development of the versatile CRISPR/Cas9 tool. However, while the isolation of isogenic cell populations is usually straightforward for mammalian cell lines, the generation of clonal <i>Drosophila</i> cell lines has remained a longstanding challenge, hampered by the difficulty of getting <i>Drosophila</i> cells to grow at low densities. Here, we describe a highly efficient workflow to generate clonal Cas9-engineered <i>Drosophila</i> cell lines using a combination of cell pools, limiting dilution in conditioned medium and PCR with allele-specific primers, enabling the efficient selection of a clonal cell line with a suitable mutation profile. We validate the protocol by documenting the isolation, selection and verification of eight independently Cas9-edited <i>armadillo</i> mutant <i>Drosophila</i> cell lines. Our method provides a powerful and simple workflow that improves the utility of <i>Drosophila</i> cells for genetic studies with CRISPR/Cas9.
DOI: 10.6084/m9.figshare.5358325
title: USGS Current Conditions Scraper

description: These two scripts can be used to pull all of the water quality and flow data from the USGS "Current Conditions" page for a given state. You will first need to build a list of URLs using the URL script, and then you can use the second script to pull all of the data from each URL. The scraper is configured to use the maximum available date range for each variable of interest (e.g. discharge, conductivity...), meaning that file sizes can be anywhere from very small to very large. If you're finding that your data is being cut-off, you'll need to extend the "timeout" parameter within the url request call (currently set to 199 s), so that the total extent of the data may load. <br>The USGS prefers automated data retrieval take place between 12 am - 6 am. Please adhere to these guidelines or your connection may be blocked. To facilitate friendly scraping, I've added a start time parameter to the second script. Set this variable ('then') to the time you want to start running the scraper. <br>Another common issue is the parameter code dictionary. The USGS maintains a large list of parameters assigned to 5 digit codes. If a code is not present in the dictionary as is (i.e. dictionary 'd'), the URL will be saved to Broken_links.csv and data will not be collected. To remedy this, follow these URLs in your browser, find the new variables/codes, and then add them to the dictionary.
DOI: 10.6084/m9.figshare.5358058
title: Hydrographic CTD data from 1993 to 2016 in the East Sea (Japan Sea) through 19 cruise surveys supported by CREAMS or EAST-I programs.

description: Using highly accurate conductivity-temperature-depth (CTD) instruments, 922 profiles of water temperature and salinity were collected in a semi-enclosed miniature ocean, the East Sea (Japan Sea), through total 19 international joint surveys between 1993­ ­and 2016 as part of the Circulation Research of the East Asian Marginal Seas program and with supports from the East Asian Seas Time-series I project.
DOI: 10.6084/m9.figshare.5358325.v1
title: USGS Surface Water Data Scraper

description: These two scripts can be used to pull all of the water quality and flow data from the USGS "Surface-Water Daily Data for the Nation" page. They are currently configured to only consider data types in coastal states and within the categories of ocean, coastal, estuary, and tidal stream. The first script concerns pulling relevant URL parameters to the location of these data, and the second script cleans the URLs and retrieves the data.
DOI: 10.6084/m9.figshare.5357977
title: Does power mobility training impact a child’s mastery motivation and spectrum of EEG activity? An exploratory project

description: <b>Purpose:</b> The purposes of this exploratory project were: (1) to evaluate the impact of power mobility training with a child who has multiple, severe impairments and (2) to determine if the child’s spectrum of electroencephalography (EEG) activity changed during power mobility training. <b>Study design:</b> A single-subject A–B–A–B research design was conducted with a four-week duration for each phase. Two target behaviours were explored: (1) mastery motivation assessed via the dimensions of mastery questionnaire (DMQ) and (2) EEG data collected under various conditions. Power mobility skills were also assessed. <b>Methods:</b> The participant was a three-year, two-month-old girl with spastic quadriplegic cerebral palsy, gross motor function classification system level V. Each target behaviour was measured weekly. During intervention phases, power mobility training was provided. <b>Results:</b> Improvements were noted in subscale scores of the DMQ. Short-term and long-term EEG changes were also noted. Improvements were noted in power mobility skills. <b>Conclusions:</b> The participant in this exploratory project demonstrated improvements in power mobility skill and function. EEG data collection procedures and variability in an individual’s EEG activity make it difficult to determine if the participant’s spectrum of EEG activity actually changed in response to power mobility training. Additional studies are needed to investigate the impact of power mobility training on the spectrum of EEG activity in children who have multiple, severe impairments.Implications for RehabilitationPower mobility training appeared to be beneficial for a child with multiple, severe impairments though the child may never become an independent, community-based power wheelchair user.Electroencephalography may be a valuable addition to the study of power mobility use in children with multiple, severe impairments.Power mobility training appeared to impact mastery motivation (the internal drive to solve complex problems and master new skills) in a child who has multiple, severe impairments. Power mobility training appeared to be beneficial for a child with multiple, severe impairments though the child may never become an independent, community-based power wheelchair user. Electroencephalography may be a valuable addition to the study of power mobility use in children with multiple, severe impairments. Power mobility training appeared to impact mastery motivation (the internal drive to solve complex problems and master new skills) in a child who has multiple, severe impairments.
DOI: 10.6084/m9.figshare.5358058.v1
title: Data_submit_Scientific_Data.zip

description: Using highly accurate conductivity-temperature-depth (CTD) instruments, 922 profiles of water temperature and salinity were collected in a semi-enclosed miniature ocean, the East Sea (Japan Sea), through total 19 international joint surveys between 1993­ ­and 2016 as part of the Circulation Research of the East Asian Marginal Seas program and with supports from the East Asian Seas Time-series I project.
DOI: 10.6084/m9.figshare.5358325.v2
title: USGS Surface Water Data Scraper

description: These two scripts can be used to pull all of the water quality and flow data from the USGS "Surface-Water Daily Data for the Nation" page. They are currently configured to only consider data types in coastal states and within the categories of ocean, coastal, estuary, and tidal stream. The first script concerns pulling relevant URL parameters to the location of these data, and the second script cleans the URLs and retrieves the data.
DOI: 10.6084/m9.figshare.5357671
title: Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers

description: Supplemental material obtained from the paper "Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers".<br>In the file "Supplementay material 1" is available the questionnaire from the social research. In the file " Supplementary material 2" is the raw data from 140 surveys. In the file "Supplementary material 3_Table S1" is the list of aquatic species and their status in Asturias together with the results from the official inventory, electrofishing results and social survey in the 4 rivers. And in file "Supplementary material 4_Table S2" is the compilation of data from official inventory, electrofishing results, social survey and releases in public media regarding non indigenous species.<br>
DOI: 10.6084/m9.figshare.5357698
title: color_figure1.mat

description: good data
DOI: 10.6084/m9.figshare.5357698.v1
title: color_figure1.mat

description: good data
DOI: 10.6084/m9.figshare.5358325.v6
title: USGS Current Conditions Scraper

description: These two scripts can be used to pull all of the water quality and flow data from the USGS "Current Conditions" page for a given state. You will first need to build a list of URLs using the URL script, and then you can use the second script to pull all of the data from each URL. The scraper is configured to use the maximum available date range for each variable of interest (e.g. discharge, conductivity...), meaning that file sizes can be anywhere from very small to very large. If you're finding that your data is being cut-off, you'll need to extend the "timeout" parameter within the url request call (currently set to 199 s), so that the total extent of the data may load. <br>The USGS prefers automated data retrieval take place between 12 am - 6 am. Please adhere to these guidelines or your connection may be blocked. To facilitate friendly scraping, I've added a start time parameter to the second script. Set this variable ('then') to the time you want to start running the scraper. <br>Another common issue is the parameter code dictionary. The USGS maintains a large list of parameters assigned to 5 digit codes. If a code is not present in the dictionary as is (i.e. dictionary 'd'), the URL will be saved to Broken_links.csv and data will not be collected. To remedy this, follow these URLs in your browser, find the new variables/codes, and then add them to the dictionary.
DOI: 10.6084/m9.figshare.5357668
title: Cockburn et al - Syringomyelia data

description: Data accompanying paper by Cockburn et al. which investigates behavioural indicators of pain in Cavalier King Charles Spaniels with or without a diagnosis of syringomyelia. The data are in one excel file which contains the following variables:<br>Dog (dog id); SyringDiagnosis (0=no syringomyelia; 1=syringomyelia); SyrinxDiam (syrinx diameter (mm)); Medication (0=no medication; 1=medication); AgeMonth (age in months); Scratching (owner VAS scratching score); EyeTemperature (degrees celsius); JumpUpAv (average time to jump up to stool (s)); JumpDownAv (average time to jump down from stool (s)); PMeanAv, NPMeanAv, MmeanAV, NNmeanAV, NmeanAv (average latencies (s) to P,NP,M,NN,N locations during judgement bias tests); AdjustedNP, AdjustedM, AdjustedNN (average adjusted latencies to ambiguous NP,M,NN locations during judgement bias tests); AdjustedAv (average adjusted latency to all 3 ambiguous locations during judgement bias tests); ArenaArea (area (width x length) of test arena); FalseNegative (latency to N location during False Negative test); FalsePositive (latency to P location during False Positive test); TrialCriterion (trials to criterion in the judgement bias test); RL1-RL12 (latencies to P location in the 12 trials of the reward loss sensitivity test); RL1minusPmeanAv (latencies to P location in the 12 trials of the reward loss sensitivity test - average latency to the P location ('baseline') during the judgement bias test); RL1to12minusPmeanAv (mean increase in latency relative to baseline across all 12 trials of the reward loss sensitivity test); RLtrialscompleted (number of RL trials completed by each dog).
DOI: 10.6084/m9.figshare.5357671.v1
title: Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers

description: Supplemental material obtained from the paper "Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers".<br>In the file "Supplementay material 1" is available the questionnaire from the social research. In the file " Supplementary material 2" is the raw data from 140 surveys. In the file "Supplementary material 3_Table S1" is the list of aquatic species and their status in Asturias together with the results from the official inventory, electrofishing results and social survey in the 4 rivers. And in file "Supplementary material 4_Table S2" is the compilation of data from official inventory, electrofishing results, social survey and releases in public media regarding non indigenous species.<br>
DOI: 10.6084/m9.figshare.5357593
title: Novel approach of laparoscopic and endoscopic cooperative surgery (LECS) for cholecystectomy

description: <b>Background:</b> Endoscopic submucosal dissection (ESD) techniques, such as generating an artificial space between digestive tract layers for safer dissection, were thought to be safer for the resection of organs in cholecystectomy. We investigated whether combinations of endoscopic techniques and laparoscopic techniques could be performed more safely and rapidly. <b>Material and methods:</b> Laparoscopic and endoscopic cooperative-cholecystectomy (LEC-chole) and conventional laparoscopic cholecystectomy (Lapa-chole) were performed in six dogs. Operation time was defined as the time from the creation of the first port to the retrieval of the resected gallbladder (GB); and GB bed dissection time was the time from local injection of natural saline to the clipping of the cystic duct. The main roles of the endoscope in LEC-chole were to obtain a sufficient cutting space via local injection of natural saline to the GB bed and to monitor the operative view without laparoscopic camera, thus omitting the umbilical port. <b>Results:</b> The operation times were 60 ± 18.3 minutes for LEC-chole and 95 ± 7.0 for Lapa-chole (<i>p</i> = .036). The GB bed dissection times were 31 ± 8.54 minutes in LEC-chole and 50.6 ± 7.37 minutes in Lapa-chole (<i>p</i> = 0.048). There were significant differences in liver damage and bleeding (<i>p</i> = 0.116), but there were no significant differences in one-month survival. <b>Conclusions:</b> The application of LEC-chole may be expanded to cholecystectomy.
DOI: 10.6084/m9.figshare.5358325.v5
title: USGS Surface Water Data Scraper

description: These two scripts can be used to pull all of the water quality and flow data from the USGS "Current Conditions" page for a given state. You will first need to build a list of URLs using the URL script, and then you can use the second script to pull all of the data from each URL. The scraper is configured to use the maximum available date range for each variable of interest (e.g. discharge, conductivity...), meaning that file sizes can be anywhere from very small to very large. If you're finding that your data is being cut-off, you'll need to extend the "timeout" parameter within the url request call (currently set to 199 s), so that the total extent of the data may load. <br>The USGS prefers automated data retrieval take place between 12 am - 6 am. Please adhere to these guidelines or your connection may be blocked. To facilitate friendly scraping, I've added a start time parameter to the second script. Set this variable ('then') to the time you want to start running the scraper. <br>Another common issue is the parameter code dictionary. The USGS maintains a large list of parameters assigned to 5 digit codes. If a code is not present in the dictionary as is (i.e. dictionary 'd'), the URL will be saved to Broken_links.csv and data will not be collected. To remedy this, follow these URLs in your browser, find the new variables/codes, and then add them to the dictionary.
DOI: 10.6084/m9.figshare.5357575
title: Raw data for sex ratios of offspring from different aged tert heterozygous parents.

description: Raw data for sex ratios of offspring from different aged tert<i><sup>sa6541/+</sup></i> heterozygous parents.<br>Numbers of male and female fish of each genotype are given for 7 lines. Parental age at time of conception is shown.<br><br>
DOI: 10.6084/m9.figshare.5357578
title: Raw data for weights of adult fish.

description: Weights of 8 month old homozygous, heterozygous and wild-type tert<i><sup>sa6541</sup></i> adult males from parents aged 4 or 16 months at time of conception and which were raised at the same time in the nursery are provided. Weights of 6 month old wild-type fish raised at the same time from parents aged 5 or 30 months at time of conception are shown.<br><br>
DOI: 10.6084/m9.figshare.5357587
title: A tree ring based summer precipitation reconstruction for eastern Finland (1550 AD to 1993 AD)

description: A tree ring based summer precipitation reconstruction for eastern Finland (1550 AD to 1993 AD), developed for the article: Aakala et al. Multiscale variation in drought controlled historical forest fire activity in the boreal forests of eastern Fennoscandia. Ecological Monographs (in press). <br>
DOI: 10.6084/m9.figshare.5357671.v2
title: Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers

description: Supplemental material obtained from the paper "Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers".<br>In the file "Supplementay material 1" is available the questionnaire from the social research. In the file " Supplementary material 2" is the raw data from 140 surveys. In the file "Supplementary material 3_Table S1" is the list of aquatic species and their status in Asturias together with the results from the official inventory, electrofishing results and social survey in the 4 rivers. And in file "Supplementary material 4_Table S2" is the compilation of data from official inventory, electrofishing results, social survey and releases in public media regarding non indigenous species.<br>
DOI: 10.6084/m9.figshare.5357593.v1
title: Novel approach of laparoscopic and endoscopic cooperative surgery (LECS) for cholecystectomy

description: <b>Background:</b> Endoscopic submucosal dissection (ESD) techniques, such as generating an artificial space between digestive tract layers for safer dissection, were thought to be safer for the resection of organs in cholecystectomy. We investigated whether combinations of endoscopic techniques and laparoscopic techniques could be performed more safely and rapidly. <b>Material and methods:</b> Laparoscopic and endoscopic cooperative-cholecystectomy (LEC-chole) and conventional laparoscopic cholecystectomy (Lapa-chole) were performed in six dogs. Operation time was defined as the time from the creation of the first port to the retrieval of the resected gallbladder (GB); and GB bed dissection time was the time from local injection of natural saline to the clipping of the cystic duct. The main roles of the endoscope in LEC-chole were to obtain a sufficient cutting space via local injection of natural saline to the GB bed and to monitor the operative view without laparoscopic camera, thus omitting the umbilical port. <b>Results:</b> The operation times were 60 ± 18.3 minutes for LEC-chole and 95 ± 7.0 for Lapa-chole (<i>p</i> = .036). The GB bed dissection times were 31 ± 8.54 minutes in LEC-chole and 50.6 ± 7.37 minutes in Lapa-chole (<i>p</i> = 0.048). There were significant differences in liver damage and bleeding (<i>p</i> = 0.116), but there were no significant differences in one-month survival. <b>Conclusions:</b> The application of LEC-chole may be expanded to cholecystectomy.
DOI: 10.6084/m9.figshare.5357671.v4
title: Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers

description: Supplemental material obtained from the paper "Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers".<br>In the file "Supplementay material 1" is available the questionnaire from the social research. In the file " Supplementary material 2" is the raw data from 140 surveys. In the file "Supplementary material 3_Table S1" is the list of aquatic species and their status in Asturias together with the results from the official inventory, electrofishing results and social survey in the 4 rivers. And in file "Supplementary material 4_Table S2" is the compilation of data from official inventory, electrofishing results, social survey and releases in public media regarding non indigenous species.<br>
DOI: 10.6084/m9.figshare.5357575.v1
title: Raw data for sex ratios of offspring from different aged tert heterozygous parents.

description: Raw data for sex ratios of offspring from different aged tert<i><sup>sa6541/+</sup></i> heterozygous parents.<br>Numbers of male and female fish of each genotype are given for 7 lines. Parental age at time of conception is shown.<br><br>
DOI: 10.6084/m9.figshare.5357578.v1
title: Raw data for weights of adult fish.

description: Weights of 8 month old homozygous, heterozygous and wild-type tert<i><sup>sa6541</sup></i> adult males from parents aged 4 or 16 months at time of conception and which were raised at the same time in the nursery are provided. Weights of 6 month old wild-type fish raised at the same time from parents aged 5 or 30 months at time of conception are shown.<br><br>
DOI: 10.6084/m9.figshare.5357569
title: Survival data for intercrosses of tert parents of different ages.

description: Survival data for intercrosses of tert<i><sup>sa6541/+</sup></i> parents of different ages.<br>
DOI: 10.6084/m9.figshare.5357671.v3
title: Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers

description: Supplemental material obtained from the paper "Public knowledge of alien species: a case study on aquatic biodiversity in North Iberian rivers".<br>In the file "Supplementay material 1" is available the questionnaire from the social research. In the file " Supplementary material 2" is the raw data from 140 surveys. In the file "Supplementary material 3_Table S1" is the list of aquatic species and their status in Asturias together with the results from the official inventory, electrofishing results and social survey in the 4 rivers. And in file "Supplementary material 4_Table S2" is the compilation of data from official inventory, electrofishing results, social survey and releases in public media regarding non indigenous species.<br>