DOI: 10.21950/lpnfcx
title: Socialization, and its modulation by sex, on the development and recovery of activity-based anorexia in rats

description: &lt;p&gt;&lt;b&gt;Description of the project&lt;/b&gt;&lt;/p&gt;

&lt;p&gt;Activity-based anorexia (ABA) is the main animal model used in the laboratory to study the role of restricted food intake and the central importance of excessive physical activity on the severe weight loss observed in the human disorder of anorexia nervosa. The procedure is usually carried out by individual isolation of animals, usually rats or mice, in a cage equipped with an activity wheel that they can access 23 hours a day and where food intake is limited to 1 h per day. Under these conditions, animals progressively perform more exercise despite eating less, and they lose weight until death unless they are withdrawn from the procedure. It is known that the impact that social stress (e.g. isolation) exerts on health is common to humans and other social mammals, where it has been observed that adverse social consequences cause pathologies with patterns parallel to those that occur in humans. In the present study, the animals’ social condition was manipulated to observe the effect of socialization in ABA development, its recovery, and the possible different influence of the variable sex on ABA development. The results allow us to deepen our knowledge of this phenomenon and how variables related to the social environment could affect the development and recovery of anorexia nervosa, in the hope that better prevention and therapy will be given to the patients that need them.&lt;/p&gt;
description: &lt;p&gt;&lt;b&gt;Description of the dataset&lt;/b&gt;&lt;/p&gt;
&lt;p&gt;Dataset contains daily measurements of weight (percentage in relation to weight on day 0), food intake (number of grams of food consumed), food anticipatory activity (FAA) (number of wheel turns during the 2 hours before food time), postprandial activity (PPA) (number of wheel turns during the 2 hours after food time), total activity (FAA+PPA), activity distribution (number of wheel turns in each of the 1-minute intervals in which time of wheel access was divided), the days that each rat remained in the procedure until reached withdrawal criteria (survival), and the days necessary to recover the initial weight of each rat. The data were obtained from the 8 groups of rats in the experiment, with n = 10 subjects per group.&lt;/p&gt;
description: &lt;p&gt;&lt;b&gt;Methodology&lt;/b&gt;&lt;/p&gt;

&lt;p&gt;The methodology of this study was experimental. The subjects were 80 Wistar Han rats 4 or 5 weeks old. After one week of quarantine and 4 days of acclimatization, the animals were semi-randomly assigned to the groups in a 2x2x2 design. The independent variables were: sex (male/female), social condition (individualization/group), and access to a running wheel (yes/no). Dependent variables were: 1) the percentage of weight, 2) grams of food intake, 3) the number of wheel turns during FAA and PPA, 4) the number of total wheel turns (FAA+PPA), 5) the number of days until reach withdrawal criteria, and 6) the number of days it took each withdrawn rat to regain its body weight in session 0.&lt;/p&gt;

&lt;p&gt;Daily weight and food intake were manually recorded in an Excel datasheet to calculate the values of independent variables. The percentage of weight loss was calculated as the bodyweight of an animal in a particular day divided for its initial weight on session 0 and multiplied by 100. Total food consumed was calculated as the amount of food supplied minus the amount of food leftover. The number of wheel turns in FAA and PPA was automatically recorded through a MED PC IV program in individual *.txt files per subject and day. The distribution of activity was registered in 1-minute intervals. These measures were repeated during the fifteen days that the experiment lasted. The number of days to withdrawal was calculated as the number of days from session 0 to the day that a rat's weight was equal to or less than 75% of its initial weight. The number of days to recovery was calculated from the withdrawal day to the day the animal's weight was equal to or greater than its weight at session 0.&lt;/p&gt;

&lt;/p&gt;The Excel *.xlsx files has been saved as CSV UTF-8 (coma-separated values) (*.csv)&lt;/p&gt;
description: MS Excel 365
description: MED-PC IV for Windows
description: SPSS 25
description: Due to translation from Spanish to the English language, the name of some groups in "Activity distribution (ZIP)" file must be interpreted as is stated below:

WGM: RGM (Group of male rats living in groups of 5 subjects with access to the running wheel)
WIM: RIM (Male rats living in isolation with access to the running wheel)
WGF: RGH (Group of female rats living in groups of 5 subjects with access to the running wheel)
WIF: RIH (Female rats living in isolation with access to the running wheel)
NGF: NGH (Group of female rats living in groups of 5 subjects without access to the running wheel)
NIF: NIH (Female rats living in isolation without access to the running wheel)
NGM (Group of male rats living in groups of 5 subjects without access to the running wheel)
NIM (Male rats living in isolation without access to the running wheel)

All groups had the same diet consisting of 1 hour of access to food per day. The groups with access to a running wheel had access to the activity two hours before and two hours after food time (FAA and PPA respectively).