cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j05b01g4
title: CIL:35403, Drosophila melanocephala, salivary gland. In Cell Image Library

description: Preparation: chemically fixed tissue; coagulating-fixative fixed tissue
Relation to intact cell: whole mounted tissue; chromosome squash
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: High Voltage (1MeV) TEM image of a squash preparation of polytene chromosomes from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 40 degrees. Grouped with it is an image of the same region taken at a tilt angle of 60 degrees, providing an oblique stereo view of the structure.
description: Salivary glands were dissected, exposed to 0.07 M KCl, fixed in 3:1 acetic acid:ethanol and squashed in 50% acetic acid. Slides were frozen in liquid nitrogen, the cover slip removed, and the slide immersed in 1% uranyl acetate for 5 min followed by dehydration in ethanol and transfer to amyl acetate. 2% parlodion in amyl acetate was spread over the slide and allowed to air dry. Areas for observation were scored, released by water immersion, picked up on formvar-coated grids and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
DOI: 10.6075/j04b308v
title: CIL:35402, Drosophila melanocephala, salivary gland. In Cell Image Library

description: Preparation: chemically fixed tissue; coagulating-fixative fixed tissue
Relation to intact cell: whole mounted tissue; chromosome squash
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Salivary glands were dissected, exposed to 0.07 M KCl, fixed in 3:1 acetic acid:ethanol and squashed in 50% acetic acid. Slides were frozen in liquid nitrogen, the cover slip removed, and the slide immersed in 1% uranyl acetate for 5 min followed by dehydration in ethanol and transfer to amyl acetate. 2% parlodion in amyl acetate was spread over the slide and allowed to air dry. Areas for observation were scored, released by water immersion, picked up on formvar-coated grids and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
description: High Voltage (1MeV) TEM image of a squash preparation of polytene chromosomes from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization.
DOI: 10.6075/j0v123fc
title: CIL:35416, Danio rerio. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label
Visualization methods: fluorescent dextran fill; bodipy ceramide
Processing history: unprocessed raw data
Data qualification: Raw
description: Start: 18 h (18 ss) End: older than 26 h Time: 10.5 h 
Objective: 20x oil immersion with electronic zoom. 
Temperature: room temperature (22 C)
description: Zebrafish midbrain hindbrain morphogenesis as seen with bodipy-ceramide and fluorescent dextran labeling. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6075/j0s46qkj
title: CIL:35414, Danio rerio. In Cell Image Library

description: Zebrafish midbrain hindbrain morphogenesis as seen with bodipy-ceramide and fluorescent dextran labeling. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label
Visualization methods: fluorescent dextran fill; bodipy ceramide
Processing history: unprocessed raw data
Data qualification: Raw
description: Start: 18 h (18 ss) End: older than 26 h Time: 10.5 h 
Objective: 20x oil immersion with electronic zoom. 
Temperature: room temperature (22 C)
DOI: 10.6075/j0hh6hq5
title: CIL:35415, Danio rerio. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label
Visualization methods: fluorescent dextran fill; bodipy ceramide
Processing history: unprocessed raw data
Data qualification: Raw
description: Start: 18 h (18 ss) End: older than 26 h Time: 10.5 h 
Objective: 20x oil immersion with electronic zoom. 
Temperature: room temperature (22 C)
description: Zebrafish midbrain hindbrain morphogenesis as seen with bodipy-ceramide and fluorescent dextran labeling. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6075/j0h41q3w
title: CIL:35412, Danio rerio, neurecto-epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label
Visualization methods: bodipy ceramide
Processing history: unprocessed raw data
description: A 4D visualization of zebrafish somitogenesis and neural keel formation. The Quicktime movie shows progressively dorsal optical sections in a segmenting embryo. Note the midline alignment of neuroepithelial cells in the presumptive floorplate region. This alignment reflects the location of the neural midline and is the first overt morphological sign of bilateral symmetry in the neural rudiment. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6075/j0n58mcg
title: CIL:35430, Mus musculus, oocyte. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2).
 
 Figure 7a, second row. doi:10.1371/journal.pone.0003338.g007
description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
DOI: 10.6075/j0k64j2t
title: CIL:35429, Mus musculus, oocyte. In Cell Image Library

description: This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue).
 
 Figure 7a, first row. doi:10.1371/journal.pone.0003338.g007
description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
DOI: 10.6075/j0tq61j0
title: CIL:35426, Mus musculus, oocyte. In Cell Image Library

description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed
Processing history: unprocessed raw data
Data qualification: Raw
description: Time-lapse microscopy of histone-RFP expressing mouse oocytes injected with siTPX2-5h, a doublestranded siRNA against TPX2, (targeting protein for Xenopus kinesin-like protein, xklp2). Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.
 
 Video S6 Found at: doi:10.1371/journal.pone.0003338.s006
DOI: 10.6075/j0cn73wz
title: CIL:35433, Mus musculus, oocyte. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
description: This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was also injected with siTACC3, a doublestranded siRNA against TACC2.
 
 Figure 7a, fifth row. doi:10.1371/journal.pone.0003338.g007
DOI: 10.6075/j07w6c63
title: CIL:35432, Mus musculus, oocyte. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2)and rescued with the coinjection of YFP-TPX2DN, a truncated version of TPX2, which lacks the 118 first amino acids.
 
 
 Figure 7a, fourth row. doi:10.1371/journal.pone.0003338.g007
description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
DOI: 10.6075/j0hd7vnr
title: CIL:35431, Mus musculus, oocyte. In Cell Image Library

description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
description: This 3 panel image displays meiosis in a mouse oocyte triple labelled for microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2)and rescued with the coinjection of YFP-TPX2.
 
 Figure 7a, third row. doi:10.1371/journal.pone.0003338.g007
description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0zg6s8b
title: CIL:35425, Mus musculus, oocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy; bright-field microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed-monomerFP
Processing history: unprocessed raw data
Data qualification: Raw
description: Time-lapse microscopy of histone-RFP expressing
 mouse oocytes injected with siCo-5h, a control siRNA. Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.
 Video S5. Found at: doi:10.1371/journal.pone.0003338.s005
description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
DOI: 10.6075/j07081fw
title: CIL:35422, Mus musculus, oocyte. In Cell Image Library

description: Time-lapse microscopy of mouse oocytes injected with a cRNA encoding YFP-TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2), expressed at high levels. Up is the transmitted light and bottom corresponds to the YFP signal. Images were taken every 15 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.
 
 Video S2. Found at: doi:10.1371/journal.pone.0003338.s002
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy; bright-field microscopy
Parameter imaged: fluorescence emission; absorption of illumination
Source of contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization methods: Yellow fluorescent proteins from Aequorea; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw
description: Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
DOI: 10.6075/j0jq0zxb
title: CIL:35444, Drosophila melanocephala, salivary gland. In Cell Image Library

description: Preparation: chemically fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment; paraformaldehyde fixation
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 0.5 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246
description: High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 55 degrees. Grouped with it is an image of the same region taken at a tilt angle of 45 degrees, providing an oblique stereo view of the structure.
DOI: 10.6075/j0dn43xk
title: CIL:35452, Drosophila melanocephala, salivary gland. In Cell Image Library

description: Salivary gland cells were squashed in 45% acetic acid, slides frozen in liquid nitrogen, cover slips removed, stained with uranyl magnesium acetate, dehydrated with ethanol, transferred to formvar coated grids, critical point dried, and imaged at 1MeV with the Wisconsin HVEM. 
 See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.
description: Preparation: coagulating-fixative fixed tissue
Relation to intact cell: whole mounted tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Low magnification of whole polytene chromosome from 3rd instar larva of Drosophila melanogaster imaged with high voltage EM at 1MeV showing the characteristic pattern of bands and interbands.
DOI: 10.6075/j0vq31m2
title: CIL:35449, Drosophila melanocephala, salivary gland. In Cell Image Library

description: Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 0.5 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246
description: Preparation: chemically fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment; paraformaldehyde fixation
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 38 degrees. Grouped with it is an image of the same region taken at a tilt angle of 46 degrees, providing an oblique stereo view of the structure.
DOI: 10.6075/j0xd10nv
title: CIL:35448, Drosophila melanocephala, salivary gland. In Cell Image Library

description: High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 46 degrees. Grouped with it is an image of the same region taken at a tilt angle of 38 degrees, providing an oblique stereo view of the structure.
description: Preparation: chemically fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment; paraformaldehyde fixation
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 0.5 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246
DOI: 10.6075/j0251h3q
title: CIL:35447, Drosophila melanocephala, salivary gland. In Cell Image Library

description: Salivary glands were dissected, exposed to 0.07 M KCl, fixed in 3:1 acetic acid:ethanol and squashed in 50% acetic acid. Slides were frozen in liquid nitrogen, the cover slip removed, and the slide immersed in 1% uranyl acetate for 5 min followed by dehydration in ethanol and transfer to amyl acetate. 2% parlodion in amyl acetate was spread over the slide and allowed to air dry. Areas for observation were scored, released by water immersion, picked up on formvar-coated grids and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246.
description: High Voltage (1MeV) TEM image of a squash preparation of polytene chromosomes from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 55 degrees. Grouped with it is an image of the same region taken at a tilt angle of 45 degrees, providing an oblique stereo view of the structure.
description: Preparation: chemically fixed tissue; coagulating-fixative fixed tissue
Relation to intact cell: whole mounted tissue; chromosome squash
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data