cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/nkzza-8nw47
title: EPEC_8770_U2OS

description: EPEC cells strain 8770 incubated with U2OS. The aim is to see bacterial nanotubes connected  .  to the eukaryotic cell.
description: <strong>Tilt Series Date:</strong> 2020-02-14</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8770, EPEC 8612 + Δbfp ΔtraD-ygeB ΔfinO-traI</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The strain EPEC 8770 is obtained from Ritesh (Ilan Rosenshine lab, the Hebrew university) and . Amit (Sigal ben Yehuda lab). U2OS was obtained from Wei Zhao in the Jensen lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS were grown on gold finders grids overnight in DMEM medium at 37 C. next day the . grids were washed with PBS and incubated with DMEM+FCS and bacteria were added (1:100) . and the grids were cultured for 3.5 hours at 37C. Then the grids were washed twice with PBS . and incubated with starvation buffer for 1 hour. The samples were plunge frozen.</p>
<strong>Sample Preparation:</strong> Samples were blotted manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-5/rawdata/</p>EPEC_U2OS_tilt8.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-5/rawdata/EPEC_U2OS_tilt8.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_U2OS_tilt8_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-5/3dimage_111033/EPEC_U2OS_tilt8_full.rec"
        > <i class="download icon"></i> Download </a></p>

        EPEC_U2OS_tilt8.mrc.mdoc,  34.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-5/rawdata/EPEC_U2OS_tilt8.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ekkhr-0qd58
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-88/rawdata/</p>atlas_30029.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-88/rawdata/atlas_30029.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30029_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-88/3dimage_88971/atlas_30029_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30029_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-88/3dimage_90286/atlas_30029_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rt2dtv
title: Occurrence Download

description: A dataset containing 74 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus viminalis",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 74 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045070-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/3pt98-fz217
title: Exp phase S. acidocaldarius

description: Collected sm2017-12-21-x dataset on Caltech Krios
description: <strong>Tilt Series Date:</strong> 2017-12-21</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD.</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-5/rawdata/</p>TS10.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-5/rawdata/TS10.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS10_4bin_SIRT90rot_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-5/3dimage_91671/TS10_4bin_SIRT90rot_DB.rec"
        > <i class="download icon"></i> Download </a></p>

        TS10_4bin_WBP_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 311.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-5/3dimage_91672/TS10_4bin_WBP_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.227ahc
title: Occurrence Download

description: A dataset containing 4750 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Phoca largha",
 "Year 2000-2025"
 ]
}

 The dataset includes 4750 records from 22 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045071-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/d1xc0-5hf33
title: LCV_Legionella pneumophila

description: THP1 cells were infected with Legionella wild type cells and thereafter phagosomes (LCV) were purified by sucrose density gradient method. Phagoseomes were fixed before shipment.
description: <strong>Tilt Series Date:</strong> 2017-06-01</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Phagosome isolation method as described by Joelle Gabay (Endnote).</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-1/rawdata/</p>LCV_201706010001.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-1/rawdata/LCV_201706010001.mrc"
        > <i class="download icon"></i> Download </a></p>

        LCV_201706010001_part55_11.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-1/3dimage_84923/LCV_201706010001_part55_11.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-06-01-1_slicer18445.png, , 696.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-06-01-1_slicer18445.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x52qc-42e21
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-121/rawdata/</p>atlas_20002.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-121/rawdata/atlas_20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_20002_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-121/3dimage_89165/atlas_20002_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_20002_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-121/3dimage_90303/atlas_20002_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z1tbm-enr32
title: Exp phase S. acidocaldarius

description: Collected sm2017-12-21-x dataset on Caltech Krios
description: <strong>Tilt Series Date:</strong> 2017-12-21</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD.</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-4/rawdata/</p>TS6_4bin_orig.mrc,  Tilt Series (Pixel Size 0.541 nm), 106.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-4/rawdata/TS6_4bin_orig.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS6_4bin_SIRT90rot_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-4/3dimage_91669/TS6_4bin_SIRT90rot_DB.rec"
        > <i class="download icon"></i> Download </a></p>

        TS6_4bin_WBP_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 347.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-4/3dimage_91670/TS6_4bin_WBP_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wq7jz-hzw46
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-89/rawdata/</p>atlas_30026.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-89/rawdata/atlas_30026.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30026_part51_4.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-89/3dimage_88970/atlas_30026_part51_4.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30026_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-89/3dimage_90287/atlas_30026_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z0rmq-4ff14
title: Exp phase S. acidocaldarius

description: Collected sm2017-12-21-x dataset on Caltech Krios. Date wrongly noted. Actual date - Dec 02, 2017
description: <strong>Tilt Series Date:</strong> 2017-12-22</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD.</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-2/rawdata/</p>TS2.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-2/rawdata/TS2.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS2_4bin_SIRT90rot_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-2/3dimage_91679/TS2_4bin_SIRT90rot_DB.rec"
        > <i class="download icon"></i> Download </a></p>

        TS2_4bin_WBP_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 320.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-2/3dimage_91680/TS2_4bin_WBP_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3hj6d-a6415
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-102/rawdata/</p>atlas_30035.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-102/rawdata/atlas_30035.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30035_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-102/3dimage_88984/atlas_30035_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30035_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-102/3dimage_90296/atlas_30035_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>