DOI: 10.5061/dryad.6t1g1jx4x
title: Data from: Blood pressure pulsations modulate central neuronal activity via mechanosensitive ion channels

description: The transmission of heartbeat through the cerebral vascular system is
 known to cause intracranial pressure pulsations. Here we report that
 arterial pressure pulsations can directly modulate central neuronal
 activity. In a semi-intact rat brain preparation, vascular pressure
 pulsations elicit correlated local field oscillations in the olfactory
 bulb (OB) mitral cell layer. These oscillations do not require synaptic
 transmission, but reflect baroreceptive transduction in mitral cells. This
 transduction is mediated by an excitatory mechanosensitive ion channel and
 modulates neuronal spiking activity. Indeed, in awake animals, the
 heartbeat entrains the activity of a subset of OB neurons within ~20 ms.
 Thus, we propose that this fast intrinsic interoceptive mechanism can
 modulate perception, e.g. during arousal, within the OB and also possibly
 across various brain areas.
description: See Methods section of associated manuscript.
description: # Data from: Blood pressure pulsations modulate central neuronal activity
 via mechanosensitive ion channels
 The dataset consists of
 tables that list (1) all the data points displayed in cumulative graphs in
 figures; these are the results of analyses such as the power of local
 field potential oscillations across different experimental conditions (2)
 the original data traces and their fast Fourier transforms (if applicable)
 shown in figure panels with exemplary recordings. These are either local
 field potential recordings over time from the phrenic nerve and the
 olfactory bulb brain tissue in our semi-intact preparation of juvenile
 rats, and/or recordings of pressure over time at the cannula (see Fig.
 1A). In addition, for Fig. 7 and Fig. S6, there are matlab files provided
 that contain the time stamps for heartbeats, inspiration and neuronal
 spiking activity from in vivo extracellular recordings of spiking activity
 in adult head fixed mice. ## Description of the data and file structure
 Field potentials are recorded in mV (millivolts) or µV (microVolts). The
 sampling rate in these recordings is 10 kHz (kiloHertz), thus the time
 resolution is 0.1 ms (milliseconds). Fourier transforms of local field
 potential recordings (LFP) are given in arbitrary units (a.u.), since they
 were normalized to the durations of the respective field potential
 recordings. The frequency resolution is given in mHz (milliHertz); it
 differs between Fourier transforms because of the different field
 potential recording times. The FFT sampling rate ∆xFFT for an LFP data
 interval of 360 s sampled at 1 kHz amounts to ∆xFFT = 2*500 Hz/360000 =
 2.8 mHz and is accordingly reduced for shorter LFP recordings. Fourier
 transforms generated for two or more different conditions within the same
 experiment were always normalized in amplitude to the same duration for
 comparison. Pressure is recorded and displayed in units of mmHg
 (millimetres Mercury), the most commonly used unit in physiology and
 medicine. mmHg can be converted into the SI unit Pa (Pascal, kg/ms2): 1
 mmHg = 133.322 Pa All tables contain the information on the type of data,
 units and sampling rate/interval of time or frequency. Abbreviations used
 in the tables (all common and also explained in the text of the paper): OB
 Olfactory Bulb LFP Local Field Potential PNA Phrenic Nerve Activity FFT
 Fast Fourier Transform, always noted with frequency range a.u. arbitrary
 units (usually refers to FFT values) GL Glomerular Layer (site of OB-LFP
 recording) EPL External Plexiform Layer (site of OB-LFP recording) MCL
 Mitral Cell Layer (site of OB-LFP recording) GCL Granule Cell Layer (site
 of OB-LFP recording) P0, P1: power amplitude of the fundamental peak P0
 and the first harmonic P1 in the FFT of the OB-LFP RMS Root Mean Square of
 the LFP, common measure for LFP activity FWHM Full Width Half Maximum,
 used to analyse the width of FFT peaks Description of individual files:
 Figure 1: Fig1 cumulative_data.xls: contains 4 sheets with recordings
 shown in Panels B,C,D,E,F (denoted in the name of the respective sheet)
 and 1 sheet with the cumulative data shown in Panel E, F (denoted as
 “Figure 1E,F cumulative”; data points shown in the panels: cells in table
 filled in blue) Figure 2: Fig2 cumulative_data.xls: contains 2 sheets with
 recordings shown in Panels A,B (denoted in the name of the respective
 sheet), 2 sheets with the cumulative data shown in Panels A,C (denoted in
 the name of the respective sheet), 1 sheet with the inverted FFTs shown in
 Panel D (denoted in the name of the respective sheet) Figure 3: Fig3
 cumulative_data.xls: contains 3 sheets with FFTs shown in top Panels A,B,C
 (denoted in the name of the respective sheet), 1 sheet with the cumulative
 data shown in Panels A,B,C bottom, including both all the data points from
 the individual experiments and their means and standard deviations
 (denoted in the name of the respective sheet) Figure 4: Fig4
 cumulative_data.xls: contains 1 sheet with the individual and mean data
 points for the 4 different pharmacological conditions in Panels A,B,C,D.
 Spike count data are missing for two of the experiments using the compound
 GsMTx because in these experiments spikes could not be detected properly.
 Figure 5: Fig5 cumulative_data.xls: contains 1 sheet with the functions
 shown in Panel A (denoted in the name of the respective sheet; the
 functions were generated in IGOR software, but any other mathematical
 graphing software could be used), and 1 sheet with the individual data
 points shown in Panel B (denoted in the name of the respective sheet)
 Figure 6: Fig6 cumulative_data.xls: contains 1 sheet with the LFP
 recording data and the spike phase histograms shown in Panel A (denoted in
 the name of the respective sheet), and 1 sheet with the individual data
 points shown in Panel C,D (denoted in the name of the respective sheet)
 Figure 7: two files: Fig7 cumulative_data.xls: contains 1 sheet with the
 cumulative data shown in Panel F,G,I (denoted in the name of the
 respective sheet), and 1 sheet with the total numbers of analysed
 heartbeats per in vivo experiment in Fig. 7 and Fig. S6 (denoted in the
 name of the respective sheet); Fig7 datainfo_invivo.mat: The file contains
 the matlab structure array 'summary' with the fields
  .Nexperiment - ID of the experiment  .animal - animal ID  .sex - sex of
 the animal  .age - age in days at the day of the recording  .type -
 experiment type         ob_ecg - recordings from ventral olfactory bulb +
 electrocardiogram         ob_ecg_NarisOcclusion - same as ob_ecg with
 naris occluded ipsilateral to the recording site        
 ob_ecg_NarisOcclusionRemoved - same as ob_ecg with naris occlusion removed
  .numsamples - number of samples per recording  .samplingrate - Sampling
 rate in Hz  .respidx - indices of inhalations in samples  .ecgidx -
 indices of heartbeats in samples  .ch_reversal - reversal channel of
 respiration related activity in the olfactory bulb (corresponds to mitral
 cell layer)  .sp - structure containing information of extracellular
 recorded spiking activity recorded from the olfactory bulb sorted into
 single units         .ts - indices of spikes in samples         .cl -
 cluster for each spike         .cids - cluster IDs         .ch - recording
 channel with peak amplitude         .fr - firing rate per single unit
         .ns - spike number per single unit         .cgs - unit
 classification (1 means good single unit; only good units contained in the
 data structure) Supplement: Figure S1: FigS1 cumulative_data.xls: contains
 1 sheet with the cumulative data sorted into the histograms shown in Panel
 A (denoted in the name of the respective sheet) Figure S2: FigS2
 cumulative_data.xls: contains 1 sheet with the cumulative data sorted into
 the histogram shown in Panel B (denoted in the name of the sheet) Figure
 S3: FigS3 cumulative_data.xls: contains 2 sheets with the cumulative data
 shown in Panels A and B,C (denoted in the name of the respective sheets)
 Figure S4: FigS4 cumulative_data.xls: contains 1 sheet with the cumulative
 data shown in the right parts of Panels A and C (denoted in the name of
 the sheet) Figure S5: FigS5 cumulative_data.xls: contains 1 sheet with the
 cumulative data shown in Panels A, B, E, G, H, I, J (denoted in the name
 of the sheet) Figure S6: two files: FigS6 cumulative_data.xls: contains 1
 sheet with the cumulative data shown in Panels A, B, D, E, G, H, J, K
 (denoted in the name of the sheet); Fig S6 datainfo_invivo_hp-pfc.mat: The
 file contains the matlab structure array 'summary' with the
 fields  .Nexperiment - ID of the experiment  .animal - animal ID  .sex -
 sex of the animal  .age - age in days at the day of the recording  .type -
 experiment type         hp_pfc_ecg - recordings from intermediate CA1 of
 the hippocampus + medial prefrontal cortex + electrocardiogram
  .numsamples - number of samples per recording  .samplingrate - Sampling
 rate in Hz  .respidx - indices of inhalations in samples  .ecgidx -
 indices of heartbeats in samples  .sp_CA1 - structure containing
 information of extracellular recorded spiking activity recorded from the
 intermediate CA1 of the hippocampus sorted into single units         .ts -
 indices of spikes in samples         .cl - cluster for each spike        
 .cids - cluster IDs         .ch - recording channel with peak amplitude
         .fr - firing rate per single unit         .ns - spike number per
 single unit         .cgs - unit classification (1 means good single unit;
 only good units contained in the data structure) .sp_PFC - structure
 containing information of extracellular recorded spiking activity recorded
 from the medial prefrontal cortex sorted into single units         .ts -
 indices of spikes in samples         .cl - cluster for each spike        
 .cids - cluster IDs         .ch - recording channel with peak amplitude
         .fr - firing rate per single unit         .ns - spike number per
 single unit         .cgs - unit classification (1 means good single unit;
 only good units contained in the data structure) ### Code/Software Matlab
 files associated with Fig7 and Fig S6: Matlab Version R2021a. Analysis
 steps are described in detail in the Methods section of the associated
 manuscript.