cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8143034.v1
title: N3ICD with the transmembrane domain can effectively inhibit EMT by correcting the position of tight/adherens junctions

description: EMT allows a polarized epithelium to lose epithelial integrity and acquire mesenchymal characteristics. Previously, we found that overexpression of the intracellular domain of Notch3 (N3ICD) can inhibit EMT in breast cancer cells. In this study, we aimed to elucidate the influence of N3ICD or N3ICD combined with the transmembrane domain (TD+N3ICD) on the expression and distribution of TJs/AJs and polar molecules. We found that although N3ICD can upregulate the expression levels of the above-mentioned molecules, TD+N3ICD can inhibit EMT more effectively than N3ICD alone. TD+N3ICD overexpression upregulated the expression of endogenous full-length Notch3 and contributed to correcting the position of TJs/AJs molecules and better acinar structures formation. Co-immunoprecipitation results showed that the upregulated endogenous full-length Notch3 could physically interact with E-ca in MDA-MB-231/pCMV-(TD+N3ICD) cells. Collectively, our data indicate that overexpression of TD+N3ICD can effectively inhibit EMT, resulting in better positioning of TJs/AJs molecules and cell-cell adhesion in breast cancer cells.
DOI: 10.6084/m9.figshare.8143037
title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase

description: PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.
DOI: 10.6084/m9.figshare.8143037.v2
title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase

description: PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.
DOI: 10.6084/m9.figshare.8143037.v1
title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase

description: We previously showed that the RNA of platelet endothelial aggregation receptor-1(PEAR1) is highly expressed at early stages of bovine muscle satellite cells（MDSCs）differentiation. However, its biological function remains unclear. Here, we first utilized western blotting and immunofluorescence techniques to detect the expression of PEAR1 in bovine MDSCs. The expression level of PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, a scratch test showed that the wound healing rate of MDSCs increased after PEAR1 overexpression and decreased upon its suppression. At the same time, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and differentiation marker gene, myogenin (MyoG), and the myotube fusion rate increased. However, when the expression of PEAR1 was suppressed, opposite results were obtained. Protein co-immunoprecipitation experiments revealed an association between PEAR1 and integrin beta-1 (ITGB1). Notably, inhibition of focal adhesion kinase (FAK) and ITGB1 repressed bovine MDSC differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation, and may provide a basis for further exploration of PEAR1 function in bovine muscle development.
DOI: 10.6084/m9.figshare.24022851
title: Additional file 1 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 1: Supplementary Table S1. GO Enrichment of ClHSP20s.
DOI: 10.6084/m9.figshare.24022851.v1
title: Additional file 1 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 1: Supplementary Table S1. GO Enrichment of ClHSP20s.
DOI: 10.6084/m9.figshare.21774547
title: Molecular characterization of chicken infectious anaemia virus (CIAV) in China during 2020–2021

description: Chicken infectious anaemia virus (CIAV) has been identified as the causative agent of chicken infectious anaemia (CIA), causing huge economic losses to the poultry industry globally. In this study, a total of 573 clinical samples were collected from 197 broiler farms in 17 provinces of China during 2020-2021. Among them, 375 samples (375/573, 65.4%) were positive for CIAV by real-time PCR. The positive rate of CIAV detection between different regions of China ranged from 46.67% (North China) to 81.25% (Central China). The nucleotide sequences of the VP1 gene were obtained for 91 CIAV strains, whole genome sequencing was successful for 72 out of 91 strains. Phylogenetic analysis based on the VP1 gene revealed that 91 CIAV strains currently circulating in China belong to three genotypes (II, IIIa and IIIb), and most of the CIAV strains belong to genotype IIIa. Phylogenetic analysis of the whole genome showed that 71 CIAV strains belong to genotype IIIa, and one strain belongs to genotype II. Sequence analysis showed several amino acid substitutions in both the VP1, VP2 and VP3 proteins. Our results enhance the understanding of the molecular characterization of CIAV infection in China. <b>RESEARCH HIGHLIGHTS</b>A molecular systematic survey of CIAV in China during 2020-2021.CIAV genotype IIIa is the predominant genotype in China. A molecular systematic survey of CIAV in China during 2020-2021. CIAV genotype IIIa is the predominant genotype in China.
DOI: 10.6084/m9.figshare.21774547.v1
title: Molecular characterization of chicken infectious anaemia virus (CIAV) in China during 2020–2021

description: Chicken infectious anaemia virus (CIAV) has been identified as the causative agent of chicken infectious anaemia (CIA), causing huge economic losses to the poultry industry globally. In this study, a total of 573 clinical samples were collected from 197 broiler farms in 17 provinces of China during 2020-2021. Among them, 375 samples (375/573, 65.4%) were positive for CIAV by real-time PCR. The positive rate of CIAV detection between different regions of China ranged from 46.67% (North China) to 81.25% (Central China). The nucleotide sequences of the VP1 gene were obtained for 91 CIAV strains, whole genome sequencing was successful for 72 out of 91 strains. Phylogenetic analysis based on the VP1 gene revealed that 91 CIAV strains currently circulating in China belong to three genotypes (II, IIIa and IIIb), and most of the CIAV strains belong to genotype IIIa. Phylogenetic analysis of the whole genome showed that 71 CIAV strains belong to genotype IIIa, and one strain belongs to genotype II. Sequence analysis showed several amino acid substitutions in both the VP1, VP2 and VP3 proteins. Our results enhance the understanding of the molecular characterization of CIAV infection in China. <b>RESEARCH HIGHLIGHTS</b>A molecular systematic survey of CIAV in China during 2020-2021.CIAV genotype IIIa is the predominant genotype in China. A molecular systematic survey of CIAV in China during 2020-2021. CIAV genotype IIIa is the predominant genotype in China.
DOI: 10.6084/m9.figshare.24022854
title: Additional file 2 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 2: Supplementary Table S2. KEGG Enrichment of ClHSP20s.
DOI: 10.6084/m9.figshare.24022854.v1
title: Additional file 2 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 2: Supplementary Table S2. KEGG Enrichment of ClHSP20s.
DOI: 10.6084/m9.figshare.24022857
title: Additional file 3 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 3: Supplemental Table S3. Critical cis-regulatory elements distributed in the promoter of ClHSP20 genes.
DOI: 10.6084/m9.figshare.24022857.v1
title: Additional file 3 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 3: Supplemental Table S3. Critical cis-regulatory elements distributed in the promoter of ClHSP20 genes.
DOI: 10.6084/m9.figshare.24022860
title: Additional file 4 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 4: Supplemental Table S4. The TPM values for different tissues of the ClHSP20 genes base on RNA-seq.
DOI: 10.6084/m9.figshare.8143094
title: Reconstruction ensemble median and 95% range

description: This file contains the the median and 95% range (2.5th and 97.5th percentiles) of the full 7000-member ensemble accross all methods of the PAGES2k Consortium 2019 global mean temperature reconstructions.<br>We strongly recommend working with the full ensemble provided in the other files in this data compilation for a more robust analysis of GMST.The columns in this table are: Year CE, raw instrumental target data, reconstruction ensemble 50th, 2.5th and 97.5th percentiles, 31-year butterworth filtered instrumental target data, 31-year butterworth filtered reconstruction 50th, 2.5th and 97.5th percentilesAll values are °C wrt 1961-1990 over the May-April annual average.
DOI: 10.6084/m9.figshare.24022860.v1
title: Additional file 4 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 4: Supplemental Table S4. The TPM values for different tissues of the ClHSP20 genes base on RNA-seq.
DOI: 10.6084/m9.figshare.24022863
title: Additional file 5 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 5: Supplemental Table S5. The TPM valuesof the ClHSP20 genes under heat and drought tratment based on RNA-seq data.
DOI: 10.6084/m9.figshare.24022863.v1
title: Additional file 5 of Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

description: Additional file 5: Supplemental Table S5. The TPM valuesof the ClHSP20 genes under heat and drought tratment based on RNA-seq data.
DOI: 10.6084/m9.figshare.8143136
title: Sensitivity of disease cluster detection to spatial scales: an analysis with the spatial scan statistic method

description: The spatial scan statistic method has been widely used for detecting disease clusters. Its results may be affected by scales, including the aggregation level of the input data and the population threshold used in the detection. Previous studies offered inconsistent findings, and few had considered both types of scales at the same time. Using 24 simulated datasets and two real disease datasets, we investigated the method’s sensitivity to the two types of scales. We aggregated the individual-level data into areal units of three levels, including county, town, and a 900 m grid. We detected clusters with three population thresholds, including 10%, 25%, and 50%. We used two measurements, distance between cluster centres and the Jaccard index, to quantify the consistency of clusters detected with different scale settings. We find: (1) the method is not greatly sensitive to the data aggregation level when the cluster is strong and in a place with high population density; (2) the method’s sensitivity to the population threshold is determined by the actual size of the true cluster; and (3) a regular grid with fine resolution is advantageous over the subjectively defined areal units. The process and findings may have broader meanings to similar spatial analyses.
DOI: 10.6084/m9.figshare.8143136.v1
title: Sensitivity of disease cluster detection to spatial scales: an analysis with the spatial scan statistic method

description: The spatial scan statistic method has been widely used for detecting disease clusters. Its results may be affected by scales, including the aggregation level of the input data and the population threshold used in the detection. Previous studies offered inconsistent findings, and few had considered both types of scales at the same time. Using 24 simulated datasets and two real disease datasets, we investigated the method’s sensitivity to the two types of scales. We aggregated the individual-level data into areal units of three levels, including county, town, and a 900 m grid. We detected clusters with three population thresholds, including 10%, 25%, and 50%. We used two measurements, distance between cluster centres and the Jaccard index, to quantify the consistency of clusters detected with different scale settings. We find: (1) the method is not greatly sensitive to the data aggregation level when the cluster is strong and in a place with high population density; (2) the method’s sensitivity to the population threshold is determined by the actual size of the true cluster; and (3) a regular grid with fine resolution is advantageous over the subjectively defined areal units. The process and findings may have broader meanings to similar spatial analyses.
DOI: 10.6084/m9.figshare.21774722
title: LncRNA-SNHG1 promotes paclitaxel resistance of gastric cancer cells through modulating the miR-216b-5p-hexokianse 2 axis

description: Gastric cancer (GC) is one of the most malignant tumors with high incidence and poor prognosis. Currently, the combination of surgery with chemo- or radiotherapy is widely applied therapeutic strategy against GC. However, development of drug resistance severely limited the clinical application of chemotherapy. Small nucleolar RNA host gene 1 (SNHG1) has been reported to be frequently overexpressed in diverse human tumors. Yet, the biological roles and mechanisms of SNHG1 in chemoresistant GC remain unclear. Expressions of lncRNA and miRNA were detected by qRT-PCR. Responses of GC cells to Taxol treatments were evaluated by cell viability assay and apoptosis assay. Glucose metabolism rate was examined by glucose uptake and extracellular acidification rate (ECAR). The lncRNA–miRNA interaction was validated by RNA pull-down assay and luciferase assays. This study reports that expressions of SNHG1 were significantly elevated in patients with GC and gastric cancer cell lines. Silencing SNHG1 effectively suppressed GC cells migration and increased the Taxol sensitivity of GC cells. Moreover, we detected remarkedly upregulated SNHG1 expression and increased glucose metabolism in Taxol resistant cell line, MKN-45 TXR. Low glucose supply rendered Taxol resistant cells more susceptible to Taxol treatment compared with that from MKN-45 parental cells. Bioinformatical analysis, RNA pull-down and luciferase assays verified that SNHG1 functioned as a ceRNA of miR-216b-5p in GC cells. Consistently, we detected miR-216b-5p was significantly downregulated in GC tumor specimens and Taxol resistant GC cells. The hexokinase 2 (<i>HK2</i>), a glucose metabolism key enzyme, was predicted and validated as a direct target of miR-216b-5p in GC cells. Finally, restoration of miR-216b-5p in SNHG1-overexpressing MKN-45 TXR cells successfully overrode the SNHG1-promoted Taxol resistance through targeting the <i>HK2-</i>glycolysis axis. This study uncovered new biological roles and molecular mechanisms of the lncRNA-SNHG1-mediated Taxol resistance of gastric cancer, suggesting targeting the SNHG1-miR-216b-5p-<i>HK2</i> axis could be a potentially therapeutic approach against chemoresistant gastric cancer.
DOI: 10.15468/dl.hbbtk9
title: Occurrence Download

description: A dataset containing 66 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bupleurum rotundifolium",
 "Country is Poland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 66 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014753-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13145/bacdive132713.20220920.7
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132713.20221219.7.1
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132714.20220920.7
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132714.20221219.7.1
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132715.20220920.7
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132715.20221219.7.1
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132716.20220920.7
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132716.20221219.7.1
title: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132717.20220920.7
title: Blastococcus colisei Hezbri et al. 2017

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132717.20221219.7.1
title: Blastococcus colisei Hezbri et al. 2017

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132718.20220920.7
title: Bordetella sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132718.20221219.7.1
title: Bordetella sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132719.20220920.7
title: Bordetella sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132719.20221219.7.1
title: Bordetella sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132720.20220920.7
title: Bradyrhizobium sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132720.20221219.7.1
title: Bradyrhizobium sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132721.20220920.7
title: Bradyrhizobium sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132721.20221219.7.1
title: Bradyrhizobium sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132722.20220920.7
title: Brevundimonas nasdae Li et al. 2004

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132722.20221219.7.1
title: Brevundimonas nasdae Li et al. 2004

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132723.20220920.7
title: Burkholderia paludis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132723.20221219.7.1
title: Burkholderia paludis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132724.20220920.7
title: Burkholderia sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132724.20221219.7.1
title: Burkholderia sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132725.20220920.7
title: Campylobacter jejuni subsp. jejuni (Jones et al. 1931) Steele and Owen 1988

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132725.20221219.7.1
title: Campylobacter jejuni subsp. jejuni (Jones et al. 1931) Steele and Owen 1988

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive132726.20220920.7
title: Chryseobacterium sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.