cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/9djqh-wxe21
title: H. pylori 26695 WT / AGS

description: Grid box YWC229
description: <strong>Tilt Series Date:</strong> 2016-11-02</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-11-02-21/rawdata/</p>20161102_YWC229_20019.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-11-02-21/rawdata/20161102_YWC229_20019.mrc"
        > <i class="download icon"></i> Download </a></p>

        20161102_YWC229_20019_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-11-02-21/3dimage_79532/20161102_YWC229_20019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6k6a6-g2z83
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2015-10-01</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> AZ145, ΔldpF Pvan-dipM gentR</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Zielinska, A., Billini, M., Moll, A., Kremer, K., Briegel, A., Izquierdo Martinez, A., Jensen, G. J. and Thanbichler, M. (2017). Lytm factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106, 419-438.grown with 0.5 mM Vanillat</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-45/3dimage_69820/</p>CaulobacterAZ145firstset0003_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-45/3dimage_69820/CaulobacterAZ145firstset0003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        CaulobacterAZ145firstset0003.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-45/rawdata/CaulobacterAZ145firstset0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.dde2ey
title: Occurrence Download

description: A dataset containing 234 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acar plicata",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 234 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007369-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/mty6x-4rs93
title: IRE1α mNeon-Green MEF

description: Cryo-CLEM
description: <strong>Tilt Series Date:</strong> 2018-07-15</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Mouse Embryonic Fibroblasts</p>
<strong>Strain:</strong> IRE1α-mNeonGreen stable expression</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD and EMAN2</p>
<strong>Collaborators and Roles:</strong> Peter Walter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Prior to cell plating, gold Quantifoil London finder grids (EMS R2/2 LF-Au-NH2) were UV treated to sterilize and coated with cell adhesion matrix. For MEFs-IRE1-mNG cell line, grids were coated in droplets of 500 ug/mL Fibronectins (Sigma-Aldrich S5171-.5MG) for 5 minutes on each side, washed in PBS, blotted and air-dried. For U2OS-IRE1-mNG cell line, grids were coated with ~4 mg/mL undiluted Collagen type I (Corning 354236) droplets for 20 minutes, washed in PBS, blotted and air-dried. Cells were seeded at 15% confluence and allowed to adhere for 8 hours and induced with Doxycycline (500 nM) for 6 or 18 hours for MEFs and U-2 OS cell lines, respectively. ER stress was then induced by treatment with 1.5 ug/mL of Tunicamycin for 2 hours.</p>
<strong>Sample Preparation:</strong> Manual Blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-27/rawdata/</p>tomography16.mrc,  Tilt Series (Pixel Size 3.7126 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-27/rawdata/tomography16.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomographysirt.rec,  Reconstruction (Pixel Size 0.0 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-27/3dimage_118528/tomographysirt.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography16_full.rec,  Reconstruction (Pixel Size 0.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-27/3dimage_118529/tomography16_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/153k1-73078
title: Pseudomonas_aeruginosa_DfliP

description: This sample was imaged to check for the presence of the tentative flagellar inner-membrane- .  embedded structures in FliP knockout
description: <strong>Tilt Series Date:</strong> 2019-05-25</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newman lab from the transposon library (mutant 10239 from . the full library excel file). Shannon Park helped me to get the cells.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were first grown on LB plate with 100 ug/ml gentamicin over night (from -80 C stock). . Next dat, one colony was inoculated in 2 ml of LB (with 100 ug/ml gentamicin) and incubated . for 16 hours at 37 C with 175 rpm. After cells were mixed with gold beads (coated with BSA), . 4 ul were added to the grids, blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> Different plunge-freezing parameters: 1) blot force 6, blot time 3s, drain time 1s. 2) blot force 3, . blot time 2s, drain time 1s. 3) blot force -5, blot time 4s, drain time 1s. 4) blot force -3, blot time . 2s, drain time 1s. All conditions were at room temperature with 100 % humidity.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-26/rawdata/</p>P_aeruginosa_DfliP34.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-26/rawdata/P_aeruginosa_DfliP34.mrc"
        > <i class="download icon"></i> Download </a></p>

        P_aeruginosa_DfliP34_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-26/3dimage_103344/P_aeruginosa_DfliP34_full.rec"
        > <i class="download icon"></i> Download </a></p>

        P_aeruginosa_DfliP34.mrc.mdoc,  32.1 kB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.yzhzyb
title: Occurrence Download

description: A dataset containing 2793 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Chrysichthys ogooensis, Chrysichthys okae, Chrysichthys ornatus, Chrysichthys platycephalus, Chrysichthys polli, Chrysichthys punctatus, Chrysichthys sharpii, Chrysichthys stappersii, Chrysichthys teugelsi, Chrysichthys thonneri, Chrysichthys thysi, Chrysichthys turkana, Chrysichthys uniformis, Chrysichthys wagenaari, Chrysichthys walkeri, Cirrhinus cirrhosus, Citharichthys stampflii, Citharidium ansorgii, Citharinops distichodoides, Citharinus citharus, Citharinus congicus, Citharinus eburneensis, Citharinus gibbosus, Citharinus latus, Citharinus macrolepis, Clariallabes attemsi, Clariallabes brevibarbis, Clariallabes centralis, Clariallabes heterocephalus, Clariallabes laticeps, Clariallabes longicauda, Clariallabes manyangae, Clariallabes melas, Clariallabes mutsindoziensis, Clariallabes petricola, Clariallabes platyprosopos, Clariallabes simeonsi, Clariallabes teugelsi, Clariallabes uelensis, Clariallabes variabilis, Clarias agboyiensis, Clarias albopunctatus, Clarias alluaudi, Clarias angolensis, Clarias anguillaris, Clarias buettikoferi, Clarias buthupogon, Clarias camerunensis, Clarias cavernicola, Clarias dhonti, Clarias dumerilii, Clarias ebriensis, Clarias engelseni, Clarias gabonensis, Clarias gariepinus, Clarias hilli, Clarias jaensis, Clarias laeviceps, Clarias lamottei, Clarias liocephalus, Clarias longior, Clarias maclareni, Clarias macromystax, Clarias ngamensis, Clarias nigromarmoratus, Clarias pachynema, Clarias platycephalus, Clarias salae, Clarias stappersii, Clarias submarginatus, Clarias theodorae, Clarias werneri, Clarotes laticeps, Clupeocharax schoutedeni, Clypeobarbus bellcrossi, Clypeobarbus bomokandi, Clypeobarbus congicus, Clypeobarbus hypsolepis, Clypeobarbus matthesi, Clypeobarbus pleuropholis, Clypeobarbus pseudognathodon, Clypeobarbus schoutedeni, Cobitis maroccana, Coelotilapia joka, Congocharax olbrechtsi, Congochromis dimidiatus, Congochromis pugnatus, Congochromis sabinae, Congochromis squamiceps, Congoglanis alula, Congolapia bilineata, Congothrissa gossei, Congresox talabonoides, Copadichromis atripinnis, Copadichromis azureus, Copadichromis borleyi, Copadichromis chizumuluensis, Copadichromis chrysonotus, Copadichromis cyaneus, Copadichromis cyanocephalus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((11.8118 -6.3344,12.1951 -6.9401,19.1382 -6.9401,19.1382 2.0599,10.1382 2.0599,10.1382 -3.5292,11.2947 -4.7801,11.675 -5.4229,11.8118 -6.3344))"
 ]
}

 The dataset includes 2793 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007371-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gk7tzv
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acar plicata",
 "Country is Israel",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007372-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/1be6s-ktd90
title: IRE1α mNeon-Green MEF

description: Cryo-CLEM
description: <strong>Tilt Series Date:</strong> 2018-07-15</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Mouse Embryonic Fibroblasts</p>
<strong>Strain:</strong> IRE1α-mNeonGreen stable expression</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD and EMAN2</p>
<strong>Collaborators and Roles:</strong> Peter Walter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Prior to cell plating, gold Quantifoil London finder grids (EMS R2/2 LF-Au-NH2) were UV treated to sterilize and coated with cell adhesion matrix. For MEFs-IRE1-mNG cell line, grids were coated in droplets of 500 ug/mL Fibronectins (Sigma-Aldrich S5171-.5MG) for 5 minutes on each side, washed in PBS, blotted and air-dried. For U2OS-IRE1-mNG cell line, grids were coated with ~4 mg/mL undiluted Collagen type I (Corning 354236) droplets for 20 minutes, washed in PBS, blotted and air-dried. Cells were seeded at 15% confluence and allowed to adhere for 8 hours and induced with Doxycycline (500 nM) for 6 or 18 hours for MEFs and U-2 OS cell lines, respectively. ER stress was then induced by treatment with 1.5 ug/mL of Tunicamycin for 2 hours.</p>
<strong>Sample Preparation:</strong> Manual Blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-28/rawdata/</p>tomography17.mrc,  Tilt Series (Pixel Size 3.7126 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-28/rawdata/tomography17.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography17new_full2k.rec,  Reconstruction (Pixel Size 0.0 nm), 5.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-28/3dimage_118530/tomography17new_full2k.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography17new_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2018-07-15-28/3dimage_118531/tomography17new_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jpy6b-5s723
title: Pseudomonas_aeruginosa_DfliP

description: This sample was imaged to check for the presence of the tentative flagellar inner-membrane- .  embedded structures in FliP knockout
description: <strong>Tilt Series Date:</strong> 2019-05-25</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newman lab from the transposon library (mutant 10239 from . the full library excel file). Shannon Park helped me to get the cells.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were first grown on LB plate with 100 ug/ml gentamicin over night (from -80 C stock). . Next dat, one colony was inoculated in 2 ml of LB (with 100 ug/ml gentamicin) and incubated . for 16 hours at 37 C with 175 rpm. After cells were mixed with gold beads (coated with BSA), . 4 ul were added to the grids, blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> Different plunge-freezing parameters: 1) blot force 6, blot time 3s, drain time 1s. 2) blot force 3, . blot time 2s, drain time 1s. 3) blot force -5, blot time 4s, drain time 1s. 4) blot force -3, blot time . 2s, drain time 1s. All conditions were at room temperature with 100 % humidity.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-27/rawdata/</p>P_aeruginosa_DfliP16.mrc,  Tilt Series (Pixel Size 0.449 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-27/rawdata/P_aeruginosa_DfliP16.mrc"
        > <i class="download icon"></i> Download </a></p>

        P_aeruginosa_DfliP16_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-27/3dimage_103324/P_aeruginosa_DfliP16_full.rec"
        > <i class="download icon"></i> Download </a></p>

        P_aeruginosa_DfliP16.mrc.mdoc,  32.2 kB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e7wcy-94c46
title: T. primitia lysozyme

description: Cells were digested with lysozyme after the OM was removed
description: <strong>Tilt Series Date:</strong> 2012-05-13</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Treponema primitia</p>
<strong>Strain:</strong> ZAS-2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-40.0°, 40.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-13-25/rawdata/</p>trepLys10006.mrc,  Tilt Series (Pixel Size 1.9 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-13-25/rawdata/trepLys10006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w817c-7ce79
title: B. subtilis delta DacA

description: wt B. subtilis with a DacA deletion mutant
description: <strong>Tilt Series Date:</strong> 2012-11-17</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-40.0°, 40.0°), step: 1°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-11-17-10/rawdata/</p>dacA0013.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-11-17-10/rawdata/dacA0013.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vkdnn-f0e89
title: P. gingivalis wild type

description: Type IX secretion system, t9ss
description: <strong>Tilt Series Date:</strong> 2020-10-16</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.</p>
<strong>Sample Preparation:</strong> Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-127/rawdata/</p>PginWT_8_125.mrc,  Tilt Series (Pixel Size 0.333 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-127/rawdata/PginWT_8_125.mrc"
        > <i class="download icon"></i> Download </a></p>

        PginWT_8_125_full.rec,  Reconstruction (Pixel Size 1.332 nm), 884.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>