DOI: 10.5061/dryad.b5mkkwhmq
title: MTT raw data for respective ganoderma chromatography fractions

description: Ganoderma is a genus of medically important fungus that contains at least
 80 species, many of which have not been properly evaluated for their
 anticancer potential.  This study was conducted to assess the
 cytotoxic activity of the mycelial biomass of Ganoderma
 weberianum-sichuanese isolated from the Lower Volta River Basin of
 Ghana.  The nuclear ribosomal internal transcribed spacer (ITS)
 region was analyzed to determine the phylogenetic position of this native
 ganoderma isolate. We then tested its cytotoxic activity against the human
 carcinoma cell line PC-3 (human prostate), Jurkat (human T lymphoblastoid
 cell line), derived from an acute T cell leukemia, and PMDC05, a
 plasmacytoid dendritic cell (pDC) derived from acute leukemia using the
 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)
 assay. The ITS phylogenetic analysis demonstrated that this native
 Ghanaian ganoderma isolate belongs to the Ganoderma weberianum-sichuanese
 species complex.  Fractions of the mycelial biomass were found to
 inhibit significantly (≤ 0.05%) the proliferation and survival of the
 three cancer cell lines, PC-3, PMDC05, and Jurkat with increasing
 concentrations and with IC50 values of 27.73 ± 5.25, 21.31 ± 2.40 and
 17.09 ± 0.86 μg/mL, respectively compared to Chang liver cells (CVCL_0238)
 with an IC50 value of 75.41 ± 1.95 μg/mL. To the best of our knowledge,
 these findings demonstrated for the first time, that specific constituents
 of Ganoderma weberianum-sichuanese are selectively cytotoxic to the three
 human cancer cell lines, suggesting their potential efficacy in the
 treatment of malignancies. Future studies to isolate and characterize the
 biologically active molecules are warranted.
description: Cell viability upon treatment with respective chromatography
 fractions of <em>Ganoderma weberianum-sichuanese</em> extracts
 was established using the MTT assay.
description: # MTT raw data for respective ganoderma chromatography fractions
 [https://doi.org/10.5061/dryad.b5mkkwhmq](https://doi.org/10.5061/dryad.b5mkkwhmq) The data presented here is the raw data for the MTT assay in an excel format. Each cell line and the respective concentrations of the respective ganoderma chromatography fractions and their concentrations are listed in the respective columns. ## Description of the data and file structure Mean and standard deviation are given for each respective fraction. **Description**  MTT Data of Extract and Fractions of *Ganoderma weberianum-sichuanese* ·      S/N: Sample number ·      Concentration (μg/ml): Concentration of dried extract and fractions in micrograms per milliliter ·      Cell Viability Percentage (%):  Cell growth or survival effect of extract and fractions measured by MTT assay ·      Chang: Chang liver cells (CVCL_0238) ·      Jurkat: human T lymphoblastoid cell line, derived from e derived from an acute T cell leukemia ·      PMDCO5: a plasmacytoid dendritic cell (pDC) derived from acute leukemia ·      PC-3: human carcinoma cell line PC-3 (human prostate) ·      Mean:  Mean values of MTT assay performed in triplicate on Jurkat, PC-3, PMDCO5, and Chang Liver cells. ·      STDEV:  standard deviations of MTT assay performed in triplicate on Jurkat, PC-3, PMDCO5, and Chang Liver cells. ·      GL-CO1: Crude ethanol extract of *Ganoderma weberianum-sichuanese* ·      GL-C1to C7: Seven fractions from crude ethanol extract of *Ganoderma weberianum-sichuanese* obtained by silica gel column chromatography using differing mixtures of solvents of increasing polarity