DOI: 10.5061/dryad.jm58p
title: Data from: Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding

description: Global biodiversity in freshwater and the oceans is declining at high
 rates. Reliable tools for assessing and monitoring aquatic biodiversity,
 especially for rare and secretive species, are important for efficient and
 timely management. Recent advances in DNA sequencing have provided a new
 tool for species detection from DNA present into the environment. In this
 study, we tested if an environmental DNA (eDNA) metabarcoding approach,
 using water samples, can be used for addressing significant questions in
 ecology and conservation. Two key aquatic vertebrate groups were targeted:
 amphibians and bony fish. The reliability of this method was cautiously
 validated in silico, in vitro, and in situ. When compared with traditional
 surveys or historical data, eDNA metabarcoding showed a much better
 detection probability overall. For amphibians, the detection probability
 with eDNA metabarcoding was 0.97 (CI = 0.90-0.99) versus 0.58 (CI =
 0.50-0.63) for traditional surveys. For fish, in 89% of the studied sites,
 the number of taxa detected using the eDNA metabarcoding approach was
 higher or identical to the number detected using traditional methods. We
 argue that the proposed DNA-based approach has the potential to become the
 next-generation tool for ecological studies and standardized biodiversity
 monitoring in a wide range of aquatic ecosystems.
description: OTU
 tablesSequence reference
 databaseFiles containing the
 sequences for the reference databases for Batrachia and Teleostei
 speciesRun1Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run2Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run3Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run4Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run5Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run6Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run7Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.Run8Compressed file
 containing: 1) Forward sequence file 2) Reverse sequence file 3) File with
 experiment name, sample name and the tags, the forward primer and the
 reverse primer sequence associated.