cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647868.v1
title: CIL:9691

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Measuring membrane tension of the in vitro CV. To measure the build up of tension by the CV membrane an in vitro CV surrounded by a thin layer of cytosol containing ATP was mounted on the tip of a holding pipette and a thin glass rod or sphere, mounted on the tip of a microcantilever, was rested on the CV's membrane. During rounding of the CV the sphere or glass rod was pushed upward which is then recorded as an output voltage of a position sensor. This can be converted into force which, in turn, can be converted into tension. The tension of the rounded CV membrane increased 35-fold over the tension of the relaxed membrane. A DVD resolution version of this is available at CIL:10409.
DOI: 10.6084/m9.figshare.647869
title: CIL:9692

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. In vivo CVC filling/expulsion cycles. A CV in a compressed cell is observed in side view undergoing cycles of filling (diastole) and expulsion (systole) of its fluid content as the CV membrane fuses then separates from the plasma membrane and with one radial arm. The radial arm fuses with the CV after systole then just before systole it again separates from the CV. As the CV empties fluid will flow into the end of the radial arm next to the CV that is called the ampullus. This fluid comes from the collecting canal of the same radial arm. There are actually 5 to 10 radial arms connected to one CV but the others are out of the focus plane or out of the field of focus. Movies were taken by Dr. Tomomi Tani with differential interference contrast optics attached to a Leica DMIRB inverted microscope. A Leica Fluotar x63 objective lens (NA = 0.7) was used. A DVD resolution version of this is available at CIL:10411.
DOI: 10.6084/m9.figshare.647866
title: CIL:9689

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Effects of applying suction to an in vitro CV. Effect of suction on the rounding/relaxing cycle of an in vitro CV of Paramecium. When a suction micropipette is placed against the membrane of the in vitro CV and a small amount of suction is applied the CV will immediately undergo rounding even though it is still in its relaxing phase. Suction induced an extra rounding irrespective of the time in the rounding/relaxing cycle that suction was applied. Suction always produces maximum rounding. A DVD resolution version of this is available at CIL:10414.
DOI: 10.6084/m9.figshare.647867
title: CIL:9690

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Profile view of the rounding/relaxing of an in vitro CV. Side view of an in vitro CV of Paramecium spontaneously rounding and relaxing while confined under mineral oil. The isolated CV will continue to round and relax for as long as 30 min if the cytosolic fluid is not diluted too extensively and there is an adequate supply of ATP available. A DVD resolution version of this is available at CIL:10412.
DOI: 10.6084/m9.figshare.647867.v1
title: CIL:9690

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Profile view of the rounding/relaxing of an in vitro CV. Side view of an in vitro CV of Paramecium spontaneously rounding and relaxing while confined under mineral oil. The isolated CV will continue to round and relax for as long as 30 min if the cytosolic fluid is not diluted too extensively and there is an adequate supply of ATP available. A DVD resolution version of this is available at CIL:10412.
DOI: 10.6084/m9.figshare.647864
title: CIL:9687

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. In vitro CVC rounding/relaxing cycles. An in vitro CV in a ruptured Paramecium cell is surrounded by cytoplasmic vesicles and partially diluted cytoplasmic fluid. The CV periodically rounds and relaxes. One radial arm is visible which can swell as it is still able to accumulate fluid and it then fuses with the CV as the CV relaxes. A DVD resolution version of this is available at CIL:10416.
DOI: 10.6084/m9.figshare.647865
title: CIL:9688

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Activity of the two parts of a dissected CV. Division and subsequent fusion of an in vitro CV extruded from a ruptured Paramecium cell. A microneedle is used to pinch the CV into two parts. Both CV parts will undergo rounding and relaxing but these cycles soon go out of phase with each other. When the microneedle is removed the two parts of the CV will again fuse and the single CV will continue to undergo rounding and relaxing. A DVD resolution version of this is available at CIL:10415.
DOI: 10.6084/m9.figshare.647869.v1
title: CIL:9692

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. In vivo CVC filling/expulsion cycles. A CV in a compressed cell is observed in side view undergoing cycles of filling (diastole) and expulsion (systole) of its fluid content as the CV membrane fuses then separates from the plasma membrane and with one radial arm. The radial arm fuses with the CV after systole then just before systole it again separates from the CV. As the CV empties fluid will flow into the end of the radial arm next to the CV that is called the ampullus. This fluid comes from the collecting canal of the same radial arm. There are actually 5 to 10 radial arms connected to one CV but the others are out of the focus plane or out of the field of focus. Movies were taken by Dr. Tomomi Tani with differential interference contrast optics attached to a Leica DMIRB inverted microscope. A Leica Fluotar x63 objective lens (NA = 0.7) was used. A DVD resolution version of this is available at CIL:10411.
DOI: 10.6084/m9.figshare.647863
title: CIL:9311

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647865.v1
title: CIL:9688

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Activity of the two parts of a dissected CV. Division and subsequent fusion of an in vitro CV extruded from a ruptured Paramecium cell. A microneedle is used to pinch the CV into two parts. Both CV parts will undergo rounding and relaxing but these cycles soon go out of phase with each other. When the microneedle is removed the two parts of the CV will again fuse and the single CV will continue to undergo rounding and relaxing. A DVD resolution version of this is available at CIL:10415.
DOI: 10.6084/m9.figshare.647861
title: CIL:9302

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647862
title: CIL:9303

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647864.v1
title: CIL:9687

description: Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. In vitro CVC rounding/relaxing cycles. An in vitro CV in a ruptured Paramecium cell is surrounded by cytoplasmic vesicles and partially diluted cytoplasmic fluid. The CV periodically rounds and relaxes. One radial arm is visible which can swell as it is still able to accumulate fluid and it then fuses with the CV as the CV relaxes. A DVD resolution version of this is available at CIL:10416.
DOI: 10.6084/m9.figshare.647860
title: CIL:10543

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This data stack corresponds to data stack: CIL# 10538, deconvolved data stack: CIL# 10539 and maximum intensity projections: CIL# 10540 and CIL# 10541. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647861.v1
title: CIL:9302

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647862.v1
title: CIL:9303

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647863.v1
title: CIL:9311

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and lie parallel to the outer surface of the central nervous system, such that sections cut tangential to the membrane surface (see CIL:9301) show the spirals but transverse sections such as this one do not. The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647858
title: CIL:10538

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to deconvolved stack: CIL# 10539, maximum intensity projection stacks: CIL# 10540 and CIL#1 10541 and raw data stack with DIC: CIL#10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647859
title: CIL:10539

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to raw data stack: CIL# 10538, maximum intensity projection stacks: CIL# 10540 and CIL# 10541 and raw data stack with DIC: CIL# 10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647860.v1
title: CIL:10543

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This data stack corresponds to data stack: CIL# 10538, deconvolved data stack: CIL# 10539 and maximum intensity projections: CIL# 10540 and CIL# 10541. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647856
title: CIL:39786

description: Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] at the gastrula stage. Embryo was split open to reveal a nice cross-section through the outer epithelial layer and the blastocoel cavity. The fertilization envelope dissolves during swimming blastula stage; therefore, cilia and microvilli can be seen at the apical end of the epithelial layer. A few migrating primary mesenchyme cells can be seen within the blastocoel matrix. This is a lower magnification of CIL:39790. This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.
DOI: 10.6084/m9.figshare.647856.v1
title: CIL:39786

description: Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] at the gastrula stage. Embryo was split open to reveal a nice cross-section through the outer epithelial layer and the blastocoel cavity. The fertilization envelope dissolves during swimming blastula stage; therefore, cilia and microvilli can be seen at the apical end of the epithelial layer. A few migrating primary mesenchyme cells can be seen within the blastocoel matrix. This is a lower magnification of CIL:39790. This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.
DOI: 10.6084/m9.figshare.647857
title: CIL:41647

description: Confocal micrograph of a Halammohydra schulzei, which is a hydra-type fresh-water cnidarian less than 1 millimeter long. 2011 Olympus BioScapes Digital Imaging Competition®.
DOI: 10.6084/m9.figshare.647858.v1
title: CIL:10538

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to deconvolved stack: CIL# 10539, maximum intensity projection stacks: CIL# 10540 and CIL#1 10541 and raw data stack with DIC: CIL#10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647859.v1
title: CIL:10539

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to raw data stack: CIL# 10538, maximum intensity projection stacks: CIL# 10540 and CIL# 10541 and raw data stack with DIC: CIL# 10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647857.v1
title: CIL:41647

description: Confocal micrograph of a Halammohydra schulzei, which is a hydra-type fresh-water cnidarian less than 1 millimeter long. 2011 Olympus BioScapes Digital Imaging Competition®.
DOI: 10.6084/m9.figshare.647851
title: CIL:35126

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of ufd-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 4 of Mouysset et al. Movie is CIL 25631 and original data file is CIL 35126.
DOI: 10.6084/m9.figshare.647852
title: CIL:35146

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.
DOI: 10.6084/m9.figshare.647853
title: CIL:35147

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 and atl-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. Downregulation of atl-1 suppressed the P1 cell division delay phenotype of cdc-48, ufd-1, and npl-4 RNAi embryos, suggesting that the DNA replication checkpoint is responsible for the delayed P1 cell division. The series corresponds to experiments shown in Fig. 2A and Movie 6 of Mouysset et al. Movie is CIL 25633 and original data file is CIL 35147.
DOI: 10.6084/m9.figshare.647854
title: CIL:35148

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The series corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 7 of Mouysset et al. Movie is CIL 25634 and original data is CIL 35148.
DOI: 10.6084/m9.figshare.647851.v1
title: CIL:35126

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of ufd-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 4 of Mouysset et al. Movie is CIL 25631 and original data file is CIL 35126.