DOI: 10.5061/dryad.tdz08kq5h
title: Toward three-dimensional DNA industrial nanorobots

description: Nanoscale industrial robots have potential as manufacturing platforms,
 capable of automatically performing repetitive tasks to handle and produce
 nanomaterials with consistent precision and accuracy. We demonstrate a DNA
 industrial nanorobot, that fabricates a three-dimensional (3D) optically
 active, chiral structure from optically inactive parts. By making use of
 externally controlled temperature and ultraviolet (UV) light, our
 programmable robot, ~ 100 nanometers in size, grabs different parts,
 positions and aligns them so that they can be "welded", releases
 the construct and returns to its original configuration ready for its next
 operation. Our robot can also self-replicate its 3D structure and
 functions, surpassing single-step templating (restricted to
 two-dimensional (2D)) by using folding to access the third dimension and
 more degrees of freedom. Our introduction of multiple-axis precise folding
 and positioning as a tool/technology for nanomanufacturing will open the
 door to more complex and useful nano-micro devices.
description: # Toward three-dimensional DNA industrial nanorobots
 [https://doi.org/10.5061/dryad.tdz08kq5h](https://doi.org/10.5061/dryad.tdz08kq5h) ## Description of the data and file structure All data needed to support the conclusions of this manuscript are included in the main text or the supplementary materials. Raw data is available here. 1\. Fig_1D_lane78_20180712-4s-4.bip is the gel image to explain the mobility difference between 2D trimer and 3D robot trimer. The lane 8 and 9, from left, were used in the main text. 2\. Fig_1G_CD spectrum_left handed.xls is the data for the CD spectrum of figure 1G, left-handed. The column B was the CD spectrum of buffer alone, used as the baseline. The column C to Y were the CD intensity data for different samples. The column Z is the average data of the CD data from column C to Y. The data used to draft the plots in the main text was column AA, substration between the average and the baseline. 3\. Fig_1G_CD spectrum_right handed.xls is the data for the CD spectrum of figure 1G, left-handed. The column B was the CD spectrum of the sample. The column C was the buffer CD, used as the baseline. The data used to draft the plots in the main text was column D, substration between the B and the baseline. 4\. Fig_2_2020-07-24-ladder-rt-4s.bip is the gel image for figure 2. We used the whole image. 5\. Fig_3B_lane56_3Cii_lane78_20180705-set1-2s.bip and Fig_3C_ilane34_iiilane12_ivlane56_20180706-set2.bip were used in Figure 3. (a) For Fig. 3B, that's the lane 5 and 6 in Fig_3B_lane56_3Cii_lane78_20180705-set1-2s.bip. (b) For Fig. 3C-i, that's the lane 3 and 4 in Fig_3C_ilane34_iiilane12_ivlane56_20180706-set2.bip. (c) For Fig. 3C-ii, that's the lane 7 and 8 in Fig_3B_lane56_3Cii_lane78_20180705-set1-2s.bip. (d) For Fig. 3C-iii, that's the lane 1 and 2 in Fig_3C_ilane34_iiilane12_ivlane56_20180706-set2.bip. (e) For Fig. 3C-iv, that's the lane 5 and 6 in Fig_3C_ilane34_iiilane12_ivlane56_20180706-set2.bip. 6\. For Fig. 4B, that's the lane 1 to 9 in Fig_4B_lane19_20191118-3-2s.bip. 7\. Fig_S7A_CD spectrum.xls is the data for the CD spectrum of AuNR buffer solution in Fig. S7A. 8\. Fig_S7B_CD spectrum_0316-fg-au.xls is the data for the CD spectrum of FG-AuNR solution in Fig. S7B. 9\. Fig_S7C_CD spectrum.xls is the data for the CD spectrum of ABC-AuNR solution in Fig. S7C. 10\. For Fig. S9, that's the lane 3 to 4 in Fig_S9_lane34_20200703-20oC-4s-2-10min.bip. 11\. For Fig. S15A, that's the lane 1 to 8 in Fig_S15A_lane18_20200702-2T-REPL-T3-48-2S-1.bip. 12\. For Fig. S16A, that's the lane 1 to 5 in Fig_S16A_lane15_20200611-TEMPERATURE-C0-2S1.bip. 13\. For Fig. S19A, that's from Fig_S19A_20230512-4s.bip. We used the whole image. 14\. For Fig. S20A, that's the lane 5 to 8 in Fig_S20A_20230523-4s.bip. 15\. For Fig. S20B, that's the lane 4 to 8 in Fig_S20B_20230525-4s.bip 16\. For Fig. S21A, C0 is from the lane 10 of Fig_S21A_lane10_20200611-TEMPERATURE-C0-2S1.bip, and C1-C4 are from lane 5 to lane 8 of the Fig_S21A_lane58_20200615-2d-c1-c4-2s-x0.bip. 17\. For Fig. S22A, that's the lane 8 to 9 in Fig_S22A_20230630-4s.bip. Software to process the data: .bip files are gel images, which can be processed by ImageJ. .xls and .txt files are circular dichoism data, which can be processed by excel and Origin. ## Code/Software The raw data underlying the results in this paper and the code to operate Adafruit feather cortex M0 microcontroller are available here.