DOI: 10.5061/dryad.p72p0
title: Data from: Galega orientalis is more diverse than Galega officinalis in Caucasus – whole-genome AFLP analysis and phylogenetics of symbiosis-related genes

description: Legume plants can obtain combined nitrogen for their growth in an
 efficient way through symbiosis with specific bacteria. The symbiosis
 between Rhizobium galegae and its host plant Galega is an interesting case
 where the plant species G. orientalis and G. officinalis form effective,
 nitrogen fixing, symbioses only with the appropriate rhizobial
 counterpart, R. galegae bv. orientalis and R. galegae bv. officinalis
 respectively. There is plenty of information available on the symbiotic
 properties of nitrogen fixing rhizobia, while more information is needed
 on the properties of the host plants. The Caucasus region in Eurasia has
 been identified as the gene centre (centre of origin) of G. orientalis,
 although both G. orientalis and G. officinalis can be found in this
 region. In this study, the diversity of these two Galega species in
 Caucasus was investigated to test the hypothesis that in this region G.
 orientalis is more diverse than G. officinalis. The amplified fragment
 length polymorphism (AFLP) fingerprinting performed here showed that the
 populations of G. orientalis and R. galegae bv. orientalis are more
 diverse than those of G. officinalis and R. galegae bv. officinalis
 respectively. These results are consistent with the centre of origin
 status of Caucasus for G. orientalis. Phylogenies of the symbiosis-related
 plant genes NORK and Nfr5 were congruent with the AFLP result from a
 diversity point of view. Finally, the results of this work indicate that
 the NORK and Nfr5 genes of Galega follow the same evolutionary pattern as
 conserved plant genes.
description: Plant AFLP
 patternsThis file contains the
 normalized AFLP patterns of the Galega accessions from Caucasus, created
 with BioNumerics. These patterns functioned as the basis for the
 curve-based analysis, used to construct the similarity
 matrix.Plant_AFLP.pdfRhizobium AFLP patternsThis file contains the normalized gel picture of the AFLP patterns for the Rhizobium galegae strains representing the R. galegae population in Caucasus. The file was created with BioNumerics and used in the curve-based analysis to construct the similarity matrix of the rhizobial AFLP analysis.Rhizobium_AFLP.pdfGalega AFLP similarity matrixThe similarity matrix obtained from the BioNumerics analysis of the Galega AFLP data.Galega_AFLP_similarity_matrix.txtRhizobium AFLP similarity matrixThe similarity matrix obtained from the BioNumerics analysis of the Rhizobium galegae AFLP data.Rhizobium_AFLP_similarity_matrix.txtNORK neighbor-joining tree of complete datasetThe neighbor-joining tree used for the codeml analyses of the NORK gene. The tree was constructed in MEGA 4, with the substitution model of Tamura-Nei + gamma 1.0 and complete deletion of gaps. Bootstrap values &gt; 70 % are shown.NORK_NJ_tree_All_Galegas.pdfNfr5 neighbor-joining tree of complete datasetThe neighbor-joining tree used for the codeml analyses of the Nfr5 gene. The tree was constructed in MEGA 4, with the substitution model of Tamura-Nei + gamma 1.0 and pairwise deletion of gaps and missing data. Bootstrap values &gt; 70 % are shown.Nfr5_NJ_tree_All_Galegas.pdf