DOI: 10.22002/enq5d-qkt05
title: Caulobacter crescentus

description: Yves Brun strain 2845: CB15-del-hfsD. 1 of 5, holdfast mutants from Yves Brun, had to convert data type with Priism. 'possible 'paired' filament; short comet-tail-type filament near concave side of cell, no other features
description: <strong>Tilt Series Date:</strong> 2005-10-04</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.5°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft, Priism</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> PYE media (RB Jensen protocol);  5ml cultures in 14ml tubes; exponential phase; see DPD notebook Sep 28-30, 2005</p>
<strong>Sample Preparation:</strong> box 642, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -1, blot 1 sec, drain 1 sec; see DPD notebook Sep 30, 2005</p>
  pdd2005-10-04-1_slicer5276.jpg: S-layer pdd2005-10-04-1_slicer5277.jpg: Peptidoglycan pdd2005-10-04-1_slicer5278.jpg: Chemoreceptor pdd2005-10-04-1_slicer5279.jpg: Filament pdd2005-10-04-1_slicer5280.jpg: Rings pdd2005-10-04-1_slicer5281.jpg: Tube pdd2005-10-04-1_slicer5283.jpg: Peptidoglycan pdd2005-10-04-1_slicer5284.jpg: Chemoreceptor pdd2005-10-04-1_slicer5285.jpg: Filament pdd2005-10-04-1_slicer5287.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-1/rawdata/</p>YB2845_05Oct04t01.mrc,  Tilt Series (Pixel Size 1.2 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-1/rawdata/YB2845_05Oct04t01.mrc"
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        05Oct04t01.rec,  Reconstruction (Pixel Size 2.4 nm), 326.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-1/3dimage_243/05Oct04t01.rec"
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        05Oct04t01_full.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-1/3dimage_244/05Oct04t01_full.rec"
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        SAVE_3k_x-24y-370.dm3,  file associated to 3D Image #244, 8.5 MB  
        
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        SAVE_3k_x-24y-370.tif,  file associated to 3D Image #244, 8.4 MB  
        
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        SAVE_3k_x101y-551.dm3,  file associated to 3D Image #244, 8.5 MB  
        
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        SAVE_3k_x101y-551.tif,  file associated to 3D Image #244, 8.4 MB  
        
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        SAVE_3k_x237y-291.dm3,  file associated to 3D Image #244, 8.6 MB  
        
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        SAVE_3k_x237y-291.tif,  file associated to 3D Image #244, 8.4 MB  
        
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        SAVE_x265y-28.tif,  file associated to 3D Image #244, 8.4 MB  
        
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        x265y-28.dm3,  file associated to 3D Image #244, 8.5 MB  
        
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