DOI: 10.5061/dryad.kh18932kn
title: Data for: Getting over it? A proteomic analysis of mechanisms driving multigenerational acclimation to organic ultraviolet filters in Daphnia magna

description: Organic ultraviolet filters (UVFs) such as avobenzone, octocrylene, and
 oxybenzone are contaminants of concern due to their widespread occurrence
 in aquatic environments. Previous research has demonstrated that these
 UVFs are toxic to aquatic invertebrates over single-generation exposures;
 however, data regarding long-term outcomes across generations of exposure
 are lacking. This study sought to identify the mechanisms of toxicity to
 novel UVF exposure in D. magna and subsequent responses across 5
 generations of continuous exposure by quantifying proteomic changes at the
 end of the F0, F1, and F3 generations. A parallel study observed toxicity
 to novel UVF exposure (&gt; 40 % mortality, 46 % decreased
 reproduction); however, toxic effects were absent after 3 generations of
 continuous exposure. Impaired metabolism and immune response processes
 were observed in the F0 generation, with decreased abundance in &gt;
 80% of altered proteins in octocrylene and oxybenzone exposures.
 Impairment of these processes was gradually reversed over subsequent
 generations, with &gt; 60 % of altered proteins demonstrating
 increased abundance by the F3 generation. An increase in chitin production
 that could reduce membrane permeability to xenobiotics and pathogens,
 along with subtle changes in metabolic processes may allow exposed
 populations to negate many of the negative effects associated with UVF
 exposure. These results offer mechanistic insights into the gradual
 acclimation of continuously exposed D. magna populations that have been
 observed in response to a variety of contaminants and further serve to
 highlight the importance of utilizing a long-term approach for studies
 seeking to model contamination risks in wild populations.
description: The "Boyd_2025_ENVIROPOLL_raw_data" file contains the raw data
 for the publication titled "Getting over it? A proteomic analysis of
 mechanisms driving multigenerational acclimation to organic ultraviolet
 filters in *Daphnia magna*", published in *Environmental Pollution*,
 2025. ### Corresponding author: Aaron Boyd
 [boyd2@ualberta.ca](mailto:boyd2@ualberta.ca) University of Alberta All
 experiments were performed in the Biological Sciences building at the
 University of Alberta in Edmonton, Canada. ## Below are the column
 descriptions for the sheet "21 d data" #### Column A
 ("Treatment") OECD CTRL = OECD water without additions (water
 control) DMSO CTRL = OECD water with 0.003% v/v dimethyl sulfoxide
 (solvent control) AVO = Avobenzone (CAS: 70356-09-1) OCT = Octocrylene
 (CAS: 6197-30-4) OXY = Oxybenzone (CAS: 131-57-7) #### Column B
 ("Replicate") Assigned sample replicate number within a
 treatment and generation (1-5) #### Column C ("Generation")
 Generation identifier of each individual daphnid. F0 = Generation 1
 (parents, first exposed generation) F1 = Generation 2 (second exposed
 generation) F2 = Generation 3 (third exposed generation) F3 = Generation 4
 (fourth exposed generation) F4 = Generation 5 (fifth exposed generation)
 #### Column D ("Concentration") Target nominal exposure
 concentration of each treatment, expressed in micrograms/litre #### Column
 E ("Start_Date") Date that each replicate began exposure, in
 DD/MM/YYYY #### Columns F-CC These columns contain the data for each
 endpoint assessed daily (reproduction, mortality) DayX_n columns contain
 the number of surviving daphnids that day. DayX_notes columns contain
 comments regarding the daily collection of data. N/A values in notes
 columns indicate that no additional observations were recorded for that
 individual beyond the data already present in the datasheet. DayX_neonates
 columns represent the number of living neonates released from the parents
 that day DayX_eggs columns represent the number of unhatched eggs that
 were released from the parents that day DayX_stillbirths columns represent
 the number of neonates released from the parents that were dead at the
 time of scoring. N/A ("not applicable") values in neonates, eggs
 and stillbirth columns indicate instances where the exposures were
 terminated early. #### Column CD ("Totalneonates") The sum of
 living neonates produced over the 21 day experiment #### Column CE
 ("Totaleggs") The sum of unhatched eggs released over the 21 day
 experiment #### Column CF ("Totalstillbirths") The sum of dead
 neonates at the time of scoring released over the 21 day experiment ####
 Column CG ("Reproductiveeffort") The sum of columns CK-CM,
 representing all reproduction over the 21 day experiment #### Column CH
 ("Male") The number of male daphnids present on day 0 ####
 Column CI ("Female") The number of female daphnids present on
 day 0 ## Below are the column descriptions for the sheet "Offspring
 LC50 data" #### Column A ("Exposure_Generation") Generation
 identifier of the daphnids exposed for the offspring LC50 experiments. F0
 = Generation 1 (first exposed generation of offspring, from unexposed
 parents) F1 = Generation 2 (second exposed generation of offspring, from
 F0 generation parents) F2 = Generation 3 (third exposed generation of
 offspring, from F1 generation parents) F3 = Generation 4 (fourth exposed
 generation of offspring, from F2 generation parents) F4 = Generation 5
 (fifth exposed generation of offspring, from F3 generation parents) F5 =
 Generation 6 (sixth exposed generation of offspring, from F4 generation
 parents) #### Column B ("Parent_generation") Generation
 identifier of the parents of the daphnids exposed for the offspring LC50
 experiments. F-1 = Unexposed generation (Parents of F0 generation) F0 =
 Generation 1 (first exposed generation of parents) F1 = Generation 2
 (second exposed generation of parents) F2 = Generation 3 (third exposed
 generation of parents) F3 = Generation 4 (fourth exposed generation of
 parents) F4 = Generation 5 (fifth exposed generation of parents) ####
 Column C ("Parent_Treatment") Treatment that the daphnid parents
 were exposed to. Corresponds to the same treatments on the "21 d data
 tab" OECD CTRL = OECD water without additions (water control) DMSO
 CTRL = OECD water with 0.003% v/v dimethyl sulfoxide (solvent control) AVO
 = Avobenzone (CAS: 70356-09-1) OCT = Octocrylene (CAS: 6197-30-4) OXY =
 Oxybenzone (CAS: 131-57-7) Unexposed = Parents from the daphnid colony
 that all other treatments of the F0 generation were produced from. ####
 Column D ("LC50_Treatment") Treatment that the daphnid offspring
 were exposed to for the LC50 tests. Copper = Copper (II) sulfate (CAS:
 7758-98-7) AVO = Avobenzone (CAS: 70356-09-1) OCT = Octocrylene (CAS:
 6197-30-4) OXY = Oxybenzone (CAS: 131-57-7) #### Column E
 ("Concentration") Target nominal exposure concentration of each
 treatment, expressed in micrograms/litre #### Column F
 ("Replicate") Assigned sample replicate number within a
 treatment and generation (1-4) #### Column G ("Time_hr") The
 time since the start of exposure that observations were recorded. ####
 Column H ("Immobilized") The number of daphnids that were alive,
 but unable to move #### Column I ("Dead") The number of dead
 daphnids #### Column J ("Effect") The sum of immobilized and
 dead daphnids (Column H + I) #### Column K ("n") The total
 number of exposed daphnids present at time = 0