cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5634730.v1
title: EVALUATION OF DIFFERENT ROUTES OF LIQUID EXFOLIATION FOR THE OBTENTION OF HEXAGONAL BORON NITRIDE NANOSHEETS

description: Hexagonal boron nitride (h-BN), graphite analogous structure, has many interesting applications due to its excellent thermal, electrical and mechanical properties. In this study, different routes of liquid exfoliation using solvent and ultrasound were used to obtain two-dimensional (2D) nanosheets of h-BN. The efficiency of the exfoliative routes was evaluated by Transmission electron microscopy (TEM) and UV-Vis spectroscopy. Results demonstrated that the technique was effective in the exfoliation of h-BN bulk and that the solvent's choice, considering an optimum surface tension of the solvent-solute system, and the rotation of the centrifugation in the selection of the exfoliated nanosheets, are crucial for the performance of the technique. The most promising exfoliation route has been found to be the dispersion of h-BN in solvent mixture (20% IPA in water) for 3 h on ultrasound, followed by centrifugation at 1500 or 3000 rpm.
DOI: 10.6084/m9.figshare.5634727
title: A model for implementation of HACCP system for prevention and control of mycotoxins during the production of red dried chili pepper

description: Abstract Hazard analysis and critical control points (HACCP) is a process control system describes the potential hazards during food production flow chart. In contrast to the conventional way of ensuring product safety by end-product testing, HACCP establishes control systems, focuses mainly on preventive measures. Quality of red dried chili pepper (RDCP) should be assured by taking special care from harvesting of the peppers to retailing of the packaged product. RDCP is susceptible to fungi growth and mycotoxin contamination depending on the atmospheric and processing conditions such as humidity, temperature, storage and drying. Mycotoxins, are toxic metabolites to human health and strict control measures are required for preventing their occurrence in RDCP. In this study, HACCP system was implemented during the production of RDCP to ensure the food safety and quality characteristics of products and to expand the market. The most important critical control points (CCPs) during the production of RDCP was determined as sorting and drying. It is evident that development and use of the GMP, GAP, GSP and hygienic rules as well as HACCP during handling and processing of dry chili pepper are a must for processing plants.
DOI: 10.6084/m9.figshare.5634727.v1
title: A model for implementation of HACCP system for prevention and control of mycotoxins during the production of red dried chili pepper

description: Abstract Hazard analysis and critical control points (HACCP) is a process control system describes the potential hazards during food production flow chart. In contrast to the conventional way of ensuring product safety by end-product testing, HACCP establishes control systems, focuses mainly on preventive measures. Quality of red dried chili pepper (RDCP) should be assured by taking special care from harvesting of the peppers to retailing of the packaged product. RDCP is susceptible to fungi growth and mycotoxin contamination depending on the atmospheric and processing conditions such as humidity, temperature, storage and drying. Mycotoxins, are toxic metabolites to human health and strict control measures are required for preventing their occurrence in RDCP. In this study, HACCP system was implemented during the production of RDCP to ensure the food safety and quality characteristics of products and to expand the market. The most important critical control points (CCPs) during the production of RDCP was determined as sorting and drying. It is evident that development and use of the GMP, GAP, GSP and hygienic rules as well as HACCP during handling and processing of dry chili pepper are a must for processing plants.
DOI: 10.6084/m9.figshare.5634721
title: Sedentary behavior as discriminator of common mental disorders in elderly

description: ABSTRACT Objective To analyze sedentary behavior (SB) as a discriminator of the Common Mental Disorders (CMD) among the elderly. Methods A cross-sectional study including 310 elderly individuals living in the municipality of Ibicuí-BA. Data were collected using a questionnaire addressing sociodemographic characteristics, physical inactivity during free time and self-reported questions about the time spent sitting during a typical weekday and weekend. A Receiver Operating Characteristic (ROC) curve was used to estimate the cut-off point of SB as a discrimination of CMD. Results The general prevalence of CMD was 55.8%, and was higher among women (66,7%). The highest values for the area under the ROC curves among SB and CMD was for males: SB/day 0,58 (95% IC = 0,49-0,67). The proposed cutoff points for SB/day were: men: &gt; 330 min/day, women: &gt; 300 min/day. Conclusion Sedentary behavior is a valid indicator for the identification of suspected CMD in the elderly, showing to be a risk factor that should be observed by health professionals.
DOI: 10.6084/m9.figshare.5634721.v1
title: Sedentary behavior as discriminator of common mental disorders in elderly

description: ABSTRACT Objective To analyze sedentary behavior (SB) as a discriminator of the Common Mental Disorders (CMD) among the elderly. Methods A cross-sectional study including 310 elderly individuals living in the municipality of Ibicuí-BA. Data were collected using a questionnaire addressing sociodemographic characteristics, physical inactivity during free time and self-reported questions about the time spent sitting during a typical weekday and weekend. A Receiver Operating Characteristic (ROC) curve was used to estimate the cut-off point of SB as a discrimination of CMD. Results The general prevalence of CMD was 55.8%, and was higher among women (66,7%). The highest values for the area under the ROC curves among SB and CMD was for males: SB/day 0,58 (95% IC = 0,49-0,67). The proposed cutoff points for SB/day were: men: &gt; 330 min/day, women: &gt; 300 min/day. Conclusion Sedentary behavior is a valid indicator for the identification of suspected CMD in the elderly, showing to be a risk factor that should be observed by health professionals.
DOI: 10.6084/m9.figshare.5634715
title: INVESTIGATIVE CASE STUDIES FOR THE PROMOTION OF CSCL IN UNDERGRADUATE CHEMISTRY TEACHING

description: This paper addresses a didactic activity based on computer-supported collaborative learning (CSCL) principles applied to a scientific communication course offered to undergraduate chemistry students. We investigated the dynamics of interactions among the students within the groups focusing on skills of conversation described in the Collaborative Learning Model developed by Soller et al. In a virtual learning environment named eduqui.info students solved investigative case studies related to environmental pollution caused by plastic and oil, replacement of aviation gasoline by ethanol and replacement of trans fat found in foods. The messages posted on eduqui.info Forum were analyzed for the identification of the students' collaboration effectiveness. The major subskills related to Collaborative Learning Model found in the students' messages were inform, argue and request. Activities like the one addressed in this study can help undergraduate chemistry students to learn and apply core scientific concepts and skills.
DOI: 10.6084/m9.figshare.5634715.v1
title: INVESTIGATIVE CASE STUDIES FOR THE PROMOTION OF CSCL IN UNDERGRADUATE CHEMISTRY TEACHING

description: This paper addresses a didactic activity based on computer-supported collaborative learning (CSCL) principles applied to a scientific communication course offered to undergraduate chemistry students. We investigated the dynamics of interactions among the students within the groups focusing on skills of conversation described in the Collaborative Learning Model developed by Soller et al. In a virtual learning environment named eduqui.info students solved investigative case studies related to environmental pollution caused by plastic and oil, replacement of aviation gasoline by ethanol and replacement of trans fat found in foods. The messages posted on eduqui.info Forum were analyzed for the identification of the students' collaboration effectiveness. The major subskills related to Collaborative Learning Model found in the students' messages were inform, argue and request. Activities like the one addressed in this study can help undergraduate chemistry students to learn and apply core scientific concepts and skills.
DOI: 10.6084/m9.figshare.5634712
title: The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil

description: Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied.
DOI: 10.6084/m9.figshare.5634712.v1
title: The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil

description: Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied.
DOI: 10.6084/m9.figshare.5634706
title: First evidence of Zika virus venereal transmission in Aedes aegypti mosquitoes

description: BACKGROUND Aedes aegypti is considered the main Zika virus (ZIKV) vector, and is thought to be responsible for the 2015-2016 outbreak in Brazil. Zika positive Ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of ZIKV may occur. OBJECTIVES In this study, we aimed to demonstrate that venereal transmission of ZIKV by Ae. aegypti can occur under laboratory conditions. METHODS Ae. aegypti collected in the city of Manaus, confirmed as negative for Zika, Dengue and Chikungunya virus by reverse transcription real-time polymerase chain reaction (RT-qPCR) (AaM3V- strain), were reared under laboratory conditions and used for the experiments. The ZIKV used in this study was isolated from a patient presenting with symptoms; ZIKV was confirmed by RT-qPCR. Experiment 1: virgin male mosquitoes of AaM3V- strain were intrathoracically inoculated with a ZIKV suspension; four days after injection, they were transferred to a cage containing virgin females of AaM3V- strain and left to copulate for five days. Experiment 2: virgin female mosquitoes of AaM3V- strain were orally infected with a ZIKV suspension by blood feeding membrane assay; nine days after blood feeding, they were placed in cages with Ae. aegypti AaM3V- virgin males and left to copulate for four days. After copulation, all mosquitoes were individually evaluated for viral infection by RT-qPCR. FINDINGS The mean infection rate in Experiment 1 and Experiment 2 was 45% and 35%, respectively. In both experiments, cycle threshold values ranged from 13 to 35, indicating the presence of viral genomes. MAIN CONCLUSION Ae. aegypti males intrathoracically inoculated with a ZIKV suspension are infected and can transmit the virus to uninfected females by mating. Moreover, Ae. aegypti females orally infected with a ZIKV suspension can transmit the virus to uninfected males by copulation. This study shows that ZIKV infection of Ae. aegypti mosquitoes occurs not only during blood feeding, but also during copulation.
DOI: 10.6084/m9.figshare.5634706.v1
title: First evidence of Zika virus venereal transmission in Aedes aegypti mosquitoes

description: BACKGROUND Aedes aegypti is considered the main Zika virus (ZIKV) vector, and is thought to be responsible for the 2015-2016 outbreak in Brazil. Zika positive Ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of ZIKV may occur. OBJECTIVES In this study, we aimed to demonstrate that venereal transmission of ZIKV by Ae. aegypti can occur under laboratory conditions. METHODS Ae. aegypti collected in the city of Manaus, confirmed as negative for Zika, Dengue and Chikungunya virus by reverse transcription real-time polymerase chain reaction (RT-qPCR) (AaM3V- strain), were reared under laboratory conditions and used for the experiments. The ZIKV used in this study was isolated from a patient presenting with symptoms; ZIKV was confirmed by RT-qPCR. Experiment 1: virgin male mosquitoes of AaM3V- strain were intrathoracically inoculated with a ZIKV suspension; four days after injection, they were transferred to a cage containing virgin females of AaM3V- strain and left to copulate for five days. Experiment 2: virgin female mosquitoes of AaM3V- strain were orally infected with a ZIKV suspension by blood feeding membrane assay; nine days after blood feeding, they were placed in cages with Ae. aegypti AaM3V- virgin males and left to copulate for four days. After copulation, all mosquitoes were individually evaluated for viral infection by RT-qPCR. FINDINGS The mean infection rate in Experiment 1 and Experiment 2 was 45% and 35%, respectively. In both experiments, cycle threshold values ranged from 13 to 35, indicating the presence of viral genomes. MAIN CONCLUSION Ae. aegypti males intrathoracically inoculated with a ZIKV suspension are infected and can transmit the virus to uninfected females by mating. Moreover, Ae. aegypti females orally infected with a ZIKV suspension can transmit the virus to uninfected males by copulation. This study shows that ZIKV infection of Ae. aegypti mosquitoes occurs not only during blood feeding, but also during copulation.
DOI: 10.6084/m9.figshare.5634700.v1
title: Simple protocol for population (Sanger) sequencing for Zika virus genomic regions

description: BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE The aim of this study was to develop a simple and robust Sanger sequencing protocol targeting ZIKV relevant genetic regions, as envelope protein and nonstructural protein 5 (NS5). In addition, phylogenetic analysis of the ZIKV strains obtained using the present protocol and their comparison with previously published NGS sequences were also carried out. METHODS Six Vero cells isolates from serum and one urine sample were available to develop the procedure. Primer sets were designed in order to conduct a nested RT-PCR and a Sanger sequencing protocols. Bayesian analysis was used to infer phylogenetic relationships. FINDINGS Seven complete ZIKV envelope protein (1,571 kb) and six partial NS5 (0,798 Kb) were obtained using the protocol, with no amplification of NS5 gene from urine sample. Two NS5 sequences presented ambiguities at positions 495 and 196. Nucleotide analysis of a Sanger sequence and consensus sequence of previously NGS study revealed 100% identity. ZIKV strains described here clustered within the Asian lineage. MAIN CONCLUSIONS The present study provided a simple and low-cost Sanger protocol to sequence relevant genes of the ZIKV genome. The identity of Sanger generated sequences with published consensus NGS support the use of Sanger method for ZIKV population studies. The regions evaluated were able to provide robust phylogenetic signals and may be used to conduct molecular epidemiological studies and monitor viral evolution.
DOI: 10.6084/m9.figshare.5634697
title: Systemic modelling and simulation: management strategy in the User Experience Design Process (UXD)

description: Abstract The gathering, analysis and evaluation of heterogeneous data, derived from the interaction of users with a myriad of different technologies and proposed solutions are critical to the Design Process for the User Experience (UXD). Hence, it is important to have a clear picture of the initial design of the interdisciplinary teams involved in the process through common and collaborative semantics, as well as a better use of the data collected from users’ assessments. Based on it, this article presents theoretical and practical support, corresponding to the DAfetU systemic modelling, a hybrid conceptual framework, structured by the existing triad in experiential contexts, aiming to gain cooperation among interdisciplinary teams to retrofit the UXD while reaping the benefits from the systemic modelling and the simulation.
DOI: 10.6084/m9.figshare.5634697.v1
title: Systemic modelling and simulation: management strategy in the User Experience Design Process (UXD)

description: Abstract The gathering, analysis and evaluation of heterogeneous data, derived from the interaction of users with a myriad of different technologies and proposed solutions are critical to the Design Process for the User Experience (UXD). Hence, it is important to have a clear picture of the initial design of the interdisciplinary teams involved in the process through common and collaborative semantics, as well as a better use of the data collected from users’ assessments. Based on it, this article presents theoretical and practical support, corresponding to the DAfetU systemic modelling, a hybrid conceptual framework, structured by the existing triad in experiential contexts, aiming to gain cooperation among interdisciplinary teams to retrofit the UXD while reaping the benefits from the systemic modelling and the simulation.
DOI: 10.6084/m9.figshare.5634691
title: Programming and scheduling sugarcane harvesting fronts: model and solution methods for large-scale problems

description: Abstract: In a recent study, optimization models were proposed for programming and scheduling sugarcane harvesting fronts. This is a complex agricultural and logistic problem comprising various factors, such as raw material maturation stage, harvesting at the agricultural unit, transporting of raw material to the plant, and milling capacities of the plant. In this study, one of the optimization models previously studied was used to represent this problem using Mixed Integer Programming (MIP) of a lot sizing and scheduling model in parallel machines with sequence dependent setup times and costs. The proposed methods are based on MIP heuristics to solve this model in a real situation of a harvest season of a typical company from this sector inspired by harvest block aggregation heuristics, relax-and-fix constructive heuristics, and fix-and-optimize improvement heuristics. To compare the performance of the heuristic methods, various experiments were conducted using different combinations and variations of these methods. Three approaches were able to produce good quality solutions. One of them is described in detail and analyzed in this study, showing promising results in terms of making programming and scheduling decisions concerning sugarcane harvesting fronts.
DOI: 10.6084/m9.figshare.5634691.v1
title: Programming and scheduling sugarcane harvesting fronts: model and solution methods for large-scale problems

description: Abstract: In a recent study, optimization models were proposed for programming and scheduling sugarcane harvesting fronts. This is a complex agricultural and logistic problem comprising various factors, such as raw material maturation stage, harvesting at the agricultural unit, transporting of raw material to the plant, and milling capacities of the plant. In this study, one of the optimization models previously studied was used to represent this problem using Mixed Integer Programming (MIP) of a lot sizing and scheduling model in parallel machines with sequence dependent setup times and costs. The proposed methods are based on MIP heuristics to solve this model in a real situation of a harvest season of a typical company from this sector inspired by harvest block aggregation heuristics, relax-and-fix constructive heuristics, and fix-and-optimize improvement heuristics. To compare the performance of the heuristic methods, various experiments were conducted using different combinations and variations of these methods. Three approaches were able to produce good quality solutions. One of them is described in detail and analyzed in this study, showing promising results in terms of making programming and scheduling decisions concerning sugarcane harvesting fronts.
DOI: 10.6084/m9.figshare.5634688
title: Optimization approaches to a routing and scheduling problem of oil tankers

description: Abstract This study analyzes a routing and scheduling problem of cabotage oil ships motivated by the actual operation of an oil company along the Brazilian coast. Maritime transportation costs from offshore platforms to coastal terminals are an important issue in the search for operational excellence in the oil industry, and the prospects for growth in oil exploration in Brazil have made operations more demanding for agile and effective decision support systems (DSS). This paper presents an optimization approach to deal with this problem consisting of a mixed integer linear (MIP) programming model and an MIP heuristic known as relax and fix. The problem is formulated as a pickup and delivery vessel routing with time windows and heterogeneous fleet which minimizes the costs of fuel consumption of ships and freight contracts. In addition to the usual routing constraints, it also considers specific restrictions of oil maritime transportation problems. Numerical experiments with this approach are presented for a set of real data of the company, confirming that the optimization method is able to find good solutions for moderate-size problem instances.