cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7513412
title: Construction and use of coagulation diagrams as a tool for continuous monitoring of flocculation in water supply

description: In this work coagulation diagrams were constructed from bench tests with the aid of jartest equipment in order to evaluate the remaining turbidity of water prepared by adding kaolin suspension (until reaching a turbidity of 25 ± 2 uT). The water was then subjected to coagulation, flocculation and sedimentation. Iron-free aluminum sulfate was used as a coagulant in dosages varying from 5 mg L-1 to 100 mg L-1 and coagulation pH varying from 3 to 9. Three different rates of sedimentation velocities were investigated (3.0; 1,5; and 0.5 cm min-1). Based upon the coagulation diagrams, satisfactory results for turbidity removal were observed with coagulation pH values between 6.5 and 7.5 and coagulant dosages greater than 15 mg L-1; high dosages of coagulant did not necessarily lead to lower remaining turbidity values. Turbidity removal efficiency was higher in lower rates of sedimentation velocities, reaching values less than 1 uT at the lowest sedimentation rate studied. The construction of coagulation diagrams using jartest equipment proved to be an effective tool for the optimization of the coagulation phases, flocculation and sedimentation in water treatment, producing flocs whose characteristics were more easily analyzed using techniques of continuous monitoring flocculation.
DOI: 10.6084/m9.figshare.7512674
title: Use of biomarkers for monitoring of waters from the Arara Stream in the city of Rio Brilhante, MS, Brazil

description: This study evaluated the mutagenicity, genotoxicity and cytotoxicity of water samples from the Arara Stream in the city of Rio Brilhante, MS, Brazil, by means of toxicological tests using biomarkers. The micronuclei test was used to evaluate mutagenicity, and the comet assay was used to evaluate genotoxicity; both tests used Astyanax altiparanae. The Allium cepa test was used to analyze cytotoxicity. The collections were performed in April and June, 2013, at three different points (points 1, 2 and 3) of the Arara Stream and at the Irrigation Tank of UFGD (point 4). The results indicated greater frequency of piscine micronucleus in the June collection than in that of April. In the Allium cepa test, the cytotoxic effect was significantly greater in the collection of April, but the genotoxicity of water showed no statistical difference between the months of collection. The greatest amount of damage in the comet assay occurred at point 4, in both collection periods. The chemical analyses revealed that the levels of cadmium, chromium, copper and nickel were higher than the values allowed by legislation. The presence of pollutants above the permitted level in the analyzed samples of the Arara Stream shows possible potential mutagenic, genotoxic and cytotoxic effects to the water bodies due to these pollutants which may or may not be caused by human action. This work may thus contribute an additional tool to the quality control of water of the Arara Stream, considering its use and importance to the city.
DOI: 10.6084/m9.figshare.7512674.v1
title: Use of biomarkers for monitoring of waters from the Arara Stream in the city of Rio Brilhante, MS, Brazil

description: This study evaluated the mutagenicity, genotoxicity and cytotoxicity of water samples from the Arara Stream in the city of Rio Brilhante, MS, Brazil, by means of toxicological tests using biomarkers. The micronuclei test was used to evaluate mutagenicity, and the comet assay was used to evaluate genotoxicity; both tests used Astyanax altiparanae. The Allium cepa test was used to analyze cytotoxicity. The collections were performed in April and June, 2013, at three different points (points 1, 2 and 3) of the Arara Stream and at the Irrigation Tank of UFGD (point 4). The results indicated greater frequency of piscine micronucleus in the June collection than in that of April. In the Allium cepa test, the cytotoxic effect was significantly greater in the collection of April, but the genotoxicity of water showed no statistical difference between the months of collection. The greatest amount of damage in the comet assay occurred at point 4, in both collection periods. The chemical analyses revealed that the levels of cadmium, chromium, copper and nickel were higher than the values allowed by legislation. The presence of pollutants above the permitted level in the analyzed samples of the Arara Stream shows possible potential mutagenic, genotoxic and cytotoxic effects to the water bodies due to these pollutants which may or may not be caused by human action. This work may thus contribute an additional tool to the quality control of water of the Arara Stream, considering its use and importance to the city.
DOI: 10.6084/m9.figshare.7509167
title: Contamination vulnerability of groundwater in the municipality of Nova Palma, Rio Grande do Sul, Brazil

description: While often imperceptible to the human eye, groundwater has a relevant role as a very important component of the hydrological cycle. This research presents a case study of the GOD system assessing the vulnerability of the aquifer to contamination. The study surveyed thirty-six groundwater extraction wells in the municipality of Palma Nova, registered and located on the edge of the Paraná Sedimentary Basin in the central region of Rio Grande do Sul, Brazil. Two aquifer systems occur in this area in both outcropping and confined zones; one is composed of volcanic flows known as the Serra Geral Aquifer System (SGAS) and the other of sedimentary sandstone rocks, known as the Guarani Aquifer System (SAG). The GOD method indicates that 8% of the wells are in the insignificant class of vulnerability, 15% in the low class, 31% in the moderate class, 31% in the high class and 15% in the extreme vulnerability class. Seven cemeteries and two gas stations were identified as pollution source points. These results could be used for land use planning by the municipality's administration as well as for the preservation of groundwater quality.
DOI: 10.6084/m9.figshare.7509167.v1
title: Contamination vulnerability of groundwater in the municipality of Nova Palma, Rio Grande do Sul, Brazil

description: While often imperceptible to the human eye, groundwater has a relevant role as a very important component of the hydrological cycle. This research presents a case study of the GOD system assessing the vulnerability of the aquifer to contamination. The study surveyed thirty-six groundwater extraction wells in the municipality of Palma Nova, registered and located on the edge of the Paraná Sedimentary Basin in the central region of Rio Grande do Sul, Brazil. Two aquifer systems occur in this area in both outcropping and confined zones; one is composed of volcanic flows known as the Serra Geral Aquifer System (SGAS) and the other of sedimentary sandstone rocks, known as the Guarani Aquifer System (SAG). The GOD method indicates that 8% of the wells are in the insignificant class of vulnerability, 15% in the low class, 31% in the moderate class, 31% in the high class and 15% in the extreme vulnerability class. Seven cemeteries and two gas stations were identified as pollution source points. These results could be used for land use planning by the municipality's administration as well as for the preservation of groundwater quality.
DOI: 10.6084/m9.figshare.7515107
title: Probabilistic analysis of the distribution of daily rainfall in the State of Paraná

description: Rainfall in Brazil has a different distribution compared with northern hemisphere countries where hydrological research employs climatic data simulators developed and calibrated for Europe and/or the USA. Thus, these simulators do not produce very satisfactory results when applied to data of Brazilian weather stations. With the aim of introducing the Mixed Exponential probability distribution as an alternative to model rainfall data in Brazil, this work probabilistically analyzed the distribution of daily rainfall data in the State of Paraná, by determining which probability density functions best fit the historical monthly series. The historical series of thirty years (1980-2009) of 29 locations were used, in order to evaluate the fit of the Exponential, Gamma, Weibull, Log-Normal, Mixed Exponential and Generalized Pareto probability distributions, based on the non-parametric Anderson-Darling and Chi-Square tests. In the analyses without the Mixed Exponential distribution, the largest p-value in the two tests occurred most frequently in the Gamma distribution, followed by the Weibull distribution. When the Mixed Exponential was included in the analysis, the largest p-value occurred most frequently in the tests of adhesion, reaching 73.85% of the time in the Anderson-Darling test and 71.84% of the time in the Chi-Square test.
DOI: 10.6084/m9.figshare.7515107.v1
title: Probabilistic analysis of the distribution of daily rainfall in the State of Paraná

description: Rainfall in Brazil has a different distribution compared with northern hemisphere countries where hydrological research employs climatic data simulators developed and calibrated for Europe and/or the USA. Thus, these simulators do not produce very satisfactory results when applied to data of Brazilian weather stations. With the aim of introducing the Mixed Exponential probability distribution as an alternative to model rainfall data in Brazil, this work probabilistically analyzed the distribution of daily rainfall data in the State of Paraná, by determining which probability density functions best fit the historical monthly series. The historical series of thirty years (1980-2009) of 29 locations were used, in order to evaluate the fit of the Exponential, Gamma, Weibull, Log-Normal, Mixed Exponential and Generalized Pareto probability distributions, based on the non-parametric Anderson-Darling and Chi-Square tests. In the analyses without the Mixed Exponential distribution, the largest p-value in the two tests occurred most frequently in the Gamma distribution, followed by the Weibull distribution. When the Mixed Exponential was included in the analysis, the largest p-value occurred most frequently in the tests of adhesion, reaching 73.85% of the time in the Anderson-Darling test and 71.84% of the time in the Chi-Square test.
DOI: 10.6084/m9.figshare.7515599
title: Manganese, zinc, cadmium, lead, mercury and chromium in leachate from a landfill in Dourados, MS, Brazil

description: This study analyzed concentrations of manganese, zinc, cadmium, lead, mercury and chrome in leachate samples collected in the landfill of Dourados, Mato Grosso do Sul, Brazil. Samples from two ponds were taken, one undergoing aerobic treatment and the other undergoing anaerobic treatment. Four collections were made between 2012 and 2013, during dry seasons and rainy seasons. Metal concentrations were determined by atomic absorption spectrometry, flame mode. Comparison between the results for both ponds and between the sample collections was done using analysis of variance with a 95% accuracy level. Analysis results revealed differences in metal concentration within the samples, with the elements manganese, zinc and lead displaying higher concentrations during rainy periods and the other elements during droughts periods. Only the pluviometric index interfered in the studied metal concentrations and there were no variations in pH and electrical conductivity noticed throughout the experiment.
DOI: 10.6084/m9.figshare.7515599.v1
title: Manganese, zinc, cadmium, lead, mercury and chromium in leachate from a landfill in Dourados, MS, Brazil

description: This study analyzed concentrations of manganese, zinc, cadmium, lead, mercury and chrome in leachate samples collected in the landfill of Dourados, Mato Grosso do Sul, Brazil. Samples from two ponds were taken, one undergoing aerobic treatment and the other undergoing anaerobic treatment. Four collections were made between 2012 and 2013, during dry seasons and rainy seasons. Metal concentrations were determined by atomic absorption spectrometry, flame mode. Comparison between the results for both ponds and between the sample collections was done using analysis of variance with a 95% accuracy level. Analysis results revealed differences in metal concentration within the samples, with the elements manganese, zinc and lead displaying higher concentrations during rainy periods and the other elements during droughts periods. Only the pluviometric index interfered in the studied metal concentrations and there were no variations in pH and electrical conductivity noticed throughout the experiment.
DOI: 10.6084/m9.figshare.7518551
title: NPK fertilization and irrigation of sunflowers in Luvisol: Vegetative behavior

description: The sunflower is one of the most important crops used for the production of biofuels. While the literature does contain some information, the hydric and nutritional requirements of sunflowers are still not completely defined. In order to correct this deficiency, an experiment was conducted at the Federal University of Campina Grande, Campina Grande, Paraíba State, Brazil, with the aim of evaluating the effect of nitrogen, phosphate, and potassium fertilization and available soil water (AW) on the behavior of Sunflower cv. Embrapa 122 V2000. The experiment consisted of 44 treatments with compound fertilizers using a Baconian Matrix (1: 0-0-0, 2: 0-80-80, 3: 80-80-80, 4: 100-80-80, 5: 60-0-80, 6: 60-100-80, 7: 60-120-80, 8: 60-80-0, 9: 60-80-80, 10: 60-80-100, 11: 60-80-120 kg ha-1 of N, P and K, respectively, and four difference quantities of available soil water (55, 70, 85 and 100%). The 9th compound fertilizer treatment was considered the reference since its doses are those used by the region's sunflower growers in Rio Grande do Norte State. The treatments were arranged in a completely randomized design with three replications. The height, stem diameter, number of leaves and leaf area of the plants were evaluated at 40 and 60 days after sowing. The results showed that the effects of available soil water and NPK fertilization on sunflower crop occurred independently for all variables except for the number of leaves. All variables increased linearly as a function of available soil water, except for leaf area. For the conditions studied, the doses 100, 80 and 80 kg ha-1 of N, P2O5 and K2O resulted in the highest growth rates, respectively.
DOI: 10.6084/m9.figshare.7518551.v1
title: NPK fertilization and irrigation of sunflowers in Luvisol: Vegetative behavior

description: The sunflower is one of the most important crops used for the production of biofuels. While the literature does contain some information, the hydric and nutritional requirements of sunflowers are still not completely defined. In order to correct this deficiency, an experiment was conducted at the Federal University of Campina Grande, Campina Grande, Paraíba State, Brazil, with the aim of evaluating the effect of nitrogen, phosphate, and potassium fertilization and available soil water (AW) on the behavior of Sunflower cv. Embrapa 122 V2000. The experiment consisted of 44 treatments with compound fertilizers using a Baconian Matrix (1: 0-0-0, 2: 0-80-80, 3: 80-80-80, 4: 100-80-80, 5: 60-0-80, 6: 60-100-80, 7: 60-120-80, 8: 60-80-0, 9: 60-80-80, 10: 60-80-100, 11: 60-80-120 kg ha-1 of N, P and K, respectively, and four difference quantities of available soil water (55, 70, 85 and 100%). The 9th compound fertilizer treatment was considered the reference since its doses are those used by the region's sunflower growers in Rio Grande do Norte State. The treatments were arranged in a completely randomized design with three replications. The height, stem diameter, number of leaves and leaf area of the plants were evaluated at 40 and 60 days after sowing. The results showed that the effects of available soil water and NPK fertilization on sunflower crop occurred independently for all variables except for the number of leaves. All variables increased linearly as a function of available soil water, except for leaf area. For the conditions studied, the doses 100, 80 and 80 kg ha-1 of N, P2O5 and K2O resulted in the highest growth rates, respectively.
DOI: 10.6084/m9.figshare.7519541
title: Determination of toxicity assays, trophic state index, and physicochemical parameters on Piracicaba River and Itapeva Stream

description: Anthropogenic activity has a great impact on aquatic environments, causing changes in biodiversity and the environment. In an attempt to determine pollution levels, we established physicochemical parameters, a trophic state index and toxicity assays. The Piracicaba River is an important water body that receives xenobiotic waste from industry, domestic activities and agriculture. These pollutants are released directly into the river or by streams like Itapeva Stream, which discharges into the river. The goals of this work were to analyze the toxicity factor for Daphnia magna (TFD), trophic state index (TSI), pH, conductivity, temperature and dissolved oxygen in the Piracicaba River and in the Itapeva Stream from one monthly collection in the months of May, June and August 2011. In the Piracicaba River was not found toxicity, while in May, June and August the TFD was 1, 8 and 1, respectively. The TSI varied from mesotrophic to eutrophic in the river and in the stream from ultraoligotrophic to mesotrophic. The medium of conductivity for the Itapeva Stream was 479.5 µS.cm-1 and for the Piracicaba River was 219.8 µS.cm-1. The dissolved oxygen in the Piracicaba River varied from 6.89 to11.36 mg.L-1 and in the Itapeva Stream from 0.92 to 6.31 mg.L-1. Based upon the results, both hydric bodies were eutrophic, and the Itapeva Stream was classified as unsuitable for maintaining aquatic life.
DOI: 10.6084/m9.figshare.7519541.v1
title: Determination of toxicity assays, trophic state index, and physicochemical parameters on Piracicaba River and Itapeva Stream

description: Anthropogenic activity has a great impact on aquatic environments, causing changes in biodiversity and the environment. In an attempt to determine pollution levels, we established physicochemical parameters, a trophic state index and toxicity assays. The Piracicaba River is an important water body that receives xenobiotic waste from industry, domestic activities and agriculture. These pollutants are released directly into the river or by streams like Itapeva Stream, which discharges into the river. The goals of this work were to analyze the toxicity factor for Daphnia magna (TFD), trophic state index (TSI), pH, conductivity, temperature and dissolved oxygen in the Piracicaba River and in the Itapeva Stream from one monthly collection in the months of May, June and August 2011. In the Piracicaba River was not found toxicity, while in May, June and August the TFD was 1, 8 and 1, respectively. The TSI varied from mesotrophic to eutrophic in the river and in the stream from ultraoligotrophic to mesotrophic. The medium of conductivity for the Itapeva Stream was 479.5 µS.cm-1 and for the Piracicaba River was 219.8 µS.cm-1. The dissolved oxygen in the Piracicaba River varied from 6.89 to11.36 mg.L-1 and in the Itapeva Stream from 0.92 to 6.31 mg.L-1. Based upon the results, both hydric bodies were eutrophic, and the Itapeva Stream was classified as unsuitable for maintaining aquatic life.
DOI: 10.25345/c5pz24
title: MassIVE MSV000087114 - Proteomic Analysis of Huntington Disease Medium Spiny Neurons Identifies Impairment in Interferon-Gamma Signaling
title: Huntington disease (HD) is a neurodegenerative disease caused by an expanded CAG repeat expansion in the Huntingtin (HTT) gene, which produces a polyglutamine (polyQ) tract that alters the function of the HTT protein. Although HTT is expressed throughout different organs, the medium spiny neurons (MSN) in the striatum are one of cell types most affected in HD. The pathogenesis of HD has not been fully characterized and very few studies have focused on defining the proteome of human HD patient-derived neurons. Here, we differentiated HD72-iPSCs and isogenic controls into MSNs and carried out quantitative proteomic analysis used two independent approaches: data-dependent acquisitions with FAIMS (FAIMS-DDA) for label free quantification (LFQ) on the Orbitrap Lumos. Using FAIMS, we identified 6,323 proteins with at least two peptides (FDR &lt; 0.01). Of these, 497 proteins were significantly altered (q-value &lt;0.05 and fold change 1.5) in the HD72-MSN compared to isogenic controls. Subsequently, we quantitatively validated protein candidates by comprehensive data-independent acquisitions (DIA) on a TripleTOF 6600 quantifying 3,106 proteins. Proteins previously known to have altered expression in HD include DNM1L, GPX7, GPD2, CACNA2D1, PKM, MDH2, CAPN1, IGF2R, MMP2, MMP14, TIMP2 and CAV1. Importantly, we identified signaling pathways not previously known to be dysregulated in human HD neurons including major histocompatibility complex class (MHC) proteins, interferon-gamma signaling, cellular senescence, ApoE signaling and regulation of cellular response to heat. Moreover, we showed that proteins involved in formation and maintenance of axons, dendrites and synapses such as Septin protein members are dysregulated in HD-MSN. Unexpectly, treating HD MSNs with recombinant IFN-gamma resulted in an increase MHC-II and DARPP-32 levels in HD MSNs suggesting a role in differentiation and survival. Our omics analysis using HD-MSN identified relevant pathways that are altered in MSN and confirm current and new therapeutic targets for HD.