DOI: 10.5061/dryad.8gtht7710
title: Spatial variance of organic matter respiration stoichiometry in the Arctic Ocean

description: This dataset supports the findings and conclusions presented by Gerace et
 al. in "Spatial variance of organic matter respiration stoichiometry
 in the Arctic Ocean." The data was measured from seawater samples
 collected aboard the US Coast Guard Cutter Healy as part of a
 Synoptic Arctic Survey cruise (HLY2202/AWS2022). The cruise started at
 Port of Dutch Harbor, Unalaska, Alaska on 4 Sept. 2022, went up to the
 geographic North Pole (90°N), and then returned to Dutch Harbor on 24 Oct.
 2022. Variables of interest featured in this dataset include particulate
 chemical oxygen demand (PCOD), organic carbon (POC), organic nitrogen
 (PON), temperature, salinity, nutrients, light availability, dissolved
 oxygen, and ice coverage. Included as well are the relative frequencies of
 plankton taxonomic compositions and gene modules (KEGG) from DNA sampling
 and short-read metagenomic sequencing.
description: # Spatial variance of organic matter respiration stoichiometry in the
 Arctic Ocean (Dataset S1) Dataset DOI:
 [10.5061/dryad.8gtht7710](10.5061/dryad.8gtht7710) ## Description of the
 data and file structure We deployed a Sea-Bird Electronics 9/11
 Conductivity, Temperature, and Depth (CTD) system fitted with a rosette of
 24 Niskin bottles (10 L capacity). Conductivity (SBE 4C), temperature (SBE
 3+), dissolved oxygen (SBE 43), and photosynthetic active radiation (PAR,
 Biospherical QSP 2350) were directly measured throughout the water
 column. We estimated values for every tenth degree of latitude and
 longitude and 1 dbar of pressure. We recorded the interpolated value at 10
 dbar and 50 dbar to represent the surface and subsurface waters,
 respectively, for each station. Sea surface temperature was also measured
 by the underway system and recorded for 110 out of 113 stations and was
 used to confirm the accuracy of the interpolated data points. Seawater was
 collected by the Niskin bottles for determining particulate organic matter
 (POM), plankton DNA, oxygen isotopes, and inorganic nutrients. The bottle
 measurements were spatially interpolated like the CTD data and the
 concentrations at 10 dbar were treated as surface water at each station. 
 Sea ice concentration was remotely-sensed from passive microwave data and
 was collected using the SMMR, SSM/I, and SSMIS satellite sensors and
 provided by the U.S. National Snow & Ice Data Center. Concentrations
 were provided in a 25 km x 25 km spatial resolution and were averaged to
 the nearest whole-degrees of latitude and longitude to estimate the daily
 concentration at each station. ### Files and variables For every file,
 "NaN" values are to be considered as not applicable or missing
 entries. #### File: POMstations\_Domain.csv **Description:** Relative
 frequency (%) of plankton domains for each of the 113 POM stations #####
 Variables * POM stations (rows), plankton domains (columns) #### File:
 POMstations\_Phylum.csv **Description:** Relative frequency (%) of
 plankton phyla for each of the 113 POM stations ##### Variables * POM
 stations (rows), plankton phyla (columns) #### File:
 POMstations\_Genus.csv **Description:** Relative frequency (%) of plankton
 genera for each of the 113 POM stations ##### Variables * POM stations
 (rows), plankton genera (columns) #### File: POMstations\_KEGGmodules.csv
 **Description:** Relative frequency (%) of KEGG modules for each of the
 113 POM stations ##### Variables * POM stations (rows), KEGG modules
 (columns) #### File: CTD\_data.csv **Description:** CTD data used for the
 spatial interpolations defining the surface and subsurface values at each
 of the 113 POM stations ##### Variables * StationID: Station ID as denoted
 by the HLY2202 cruise report * BottleNum: Niskin bottle ID number * year:
 sampling year * month: sampling month * day: sampling day (UTC) * hour:
 sampling hour (UTC) * minute: sampling minute (UTC) * second: sampling
 second (UTC) * Pressure: pressure where measurement occurred (dbar) *
 Depth: depth where measurement occurred (m) * Temperature: sea temperature
 (degrees Celsius) * O2: dissolved oxygen concentration (umol/kg) * PAR:
 photosynthetically active radiation (umol per cm squared per s) *
 Latitude: degrees latitude (N) * Longitude: degrees longitude (E) *
 Salinity: sea salinity (psu) * Silicate: silicate concentration (umol/kg)
 * Nitrite + Nitrate: nitrite and nitrate concentration (umol/kg) *
 Phosphate: phosphate concentration (umol/kg) * Ammonia: ammonia
 concentration (umol/kg) * O18: delta-oxygen-18 (parts per thousand) ####
 File: POM\_Replicates.csv **Description:** Sampling times and locations of
 each POM filter ##### Variables * POM_filter: POM filter number *
 POM_station: POM station number * PCOD: particulate chemical oxygen demand
 (uM) * POC: particulate organic carbon (uM) * PON: particulate organic
 nitrogen (uM) * Lat: degrees latitude (N) * Lon: degrees longitude (E) *
 Year_UTC: sampling year * Month_UTC: sampling month * Day_UTC: sampling
 day * Hour_UTC: sampling hour in UTC * Hour_Local: sampling hour in local
 time * Underway_temp: sea temperature from underway (degrees Celsius) *
 POCN_vol: volume of seawater filtered for POC/N (L) * PCOD_vol: volume of
 seawater filtered for PCOD (L) * Prefilter: 0 = no prefilter, 1 = seawater
 was prefiltered with a 30 um mesh #### File: POM\_Master.csv
 **Description:** Master sheet of POM concentrations, respiration
 stoichiometry, and environmental conditions for each of the 113 POM
 sampling stations (surface depth only) ##### Variables * POM_station: POM
 station number * Lat: degrees latitude (N) * Lon: degrees longitude (E) *
 PCOD: particulate chemical oxygen demand (uM) * POC: particulate organic
 carbon (uM) * PON: particulate organic nitrogen (uM) * RQ: respiration
 quotient (PCOD:POC) * CN: C:N ratio (POC:PON) * totalRQ: total respiration
 quotient [(PCOD + 2PON):POC] * NO3: nitrite + nitrate (umol/kg) * NH3:
 ammonium (umol/kg) * PO4: phosphate (umol/kg) * SiO4: silicate (umol/kg) *
 O18: delta-oxygen-18 (parts per thousand) * SST: sea surface temperature
 (degrees Celsius) * SSS: sea surface salinity (psu) * PAR:
 photosynthetically active radiation (umol per cm squared per s) * O2:
 dissolved oxygen (umol/kg) * CellSize: cell size proxy from the relative
 percent of prokaryotes divided by relative percent of eukaryotes
 [log10(%:%)] * Ice: sea ice concentration (factor of 100%) #### File:
 DNA\_info.csv **Description:** Correspondence between each POM and DNA
 sampling, and necessary information for understanding the raw DNA sequence
 files in NCBI ##### Variables * POM_station: POM station number *
 POM_filter_match: corresponding POM filter (same sampling location and
 time) * DNA_volume: seawater filtered (L) * biosample_accession: BioSample
 Accession number on NCBI * library_ID: library identification name *
 title: sample title * library_strategy: SRA library strategy *
 library_source: SRA library source * library_selection: SRA library
 selection * library_layout: SRA library layout * platform: instrument make
 * instrument_model: instrument model * design_description: condensed
 methods of DNA sampling and sequencing * filetype: file type * filename:
 first replicate file name * filename2: second replicate file name