DOI: 10.5061/dryad.hh375
title: Data from: Quantitative multi-parametric magnetic resonance imaging of tumor response to photodynamic therapy

description: Objective: The aim of this study was to characterize response to
 photodynamic therapy (PDT) in a mouse cancer model using a
 multi-parametric quantitative MRI protocol and to identify MR parameters
 as potential biomarkers for early assessment of treatment outcome.
 Methods: CT26.WT colon carcinoma tumors were grown subcutaneously in the
 hind limb of BALB/c mice. Therapy consisted of intravenous injection of
 the photosensitizer Bremachlorin, followed by 10 min laser illumination
 (200 mW/cm2) of the tumor 6 h post injection. MRI at 7 T was performed at
 baseline, directly after PDT, as well as at 24 h, and 72 h. Tumor
 relaxation time constants (T1 and T2) and apparent diffusion coefficient
 (ADC) were quantified at each time point. Additionally, Gd-DOTA dynamic
 contrast-enhanced (DCE) MRI was performed to estimate transfer constants
 (Ktrans) and volume fractions of the extravascular extracellular space
 (ve) using standard Tofts-Kermode tracer kinetic modeling. At the end of
 the experiment, tumor viability was characterized by histology using
 NADH-diaphorase staining. Results: The therapy induced extensive cell
 death in the tumor and resulted in significant reduction in tumor growth,
 as compared to untreated controls. Tumor T1 and T2 relaxation times
 remained unchanged up to 24 h, but decreased at 72 h after treatment.
 Tumor ADC values significantly increased at 24 h and 72 h. DCE-MRI derived
 tracer kinetic parameters displayed an early response to the treatment.
 Directly after PDT complete vascular shutdown was observed in large parts
 of the tumors and reduced uptake (decreased Ktrans) in remaining tumor
 tissue. At 24 h, contrast uptake in most tumors was essentially absent.
 Out of 5 animals that were monitored for 2 weeks after treatment, 3 had
 tumor recurrence, in locations that showed strong contrast uptake at 72 h.
 Conclusion: DCE-MRI is an effective tool for visualization of vascular
 effects directly after PDT. Endogenous contrast parameters T1, T2, and
 ADC, measured at 24 to 72 h after PDT, are also potential biomarkers for
 evaluation of therapy outcome.
description: Animal
 overviewOverview of animals in
 the study.animal_overview.xlsx13-MI11515-01MMagnetic resonance imaging data of animal 13-MI11515-01M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11515-02MMagnetic resonance imaging data of animal 13-MI11515-02M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11515-03MMagnetic resonance imaging data of animal 13-MI11515-03M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11515-04MMagnetic resonance imaging data of animal 13-MI11515-04M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11515-05MMagnetic resonance imaging data of animal 13-MI11515-05M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11993-01MMagnetic resonance imaging data of animal 13-MI11993-01M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11993-02MMagnetic resonance imaging data of animal 13-MI11993-02M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11993-03MMagnetic resonance imaging data of animal 13-MI11993-03M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11993-04MMagnetic resonance imaging data of animal 13-MI11993-04M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.13-MI11993-05MMagnetic resonance imaging data of animal 13-MI11993-05M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI11768-01MMagnetic resonance imaging data of animal 14-MI11768-01M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI11768-02MMagnetic resonance imaging data of animal 14-MI11768-02M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI11768-03MMagnetic resonance imaging data of animal 14-MI11768-03M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI11768-04MMagnetic resonance imaging data of animal 14-MI11768-04M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI11768-05MMagnetic resonance imaging data of animal 14-MI11768-05M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI11768-07MMagnetic resonance imaging data of animal 14-MI11768-07M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-01MMagnetic resonance imaging data of animal 14-MI12026-01M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-02MMagnetic resonance imaging data of animal 14-MI12026-02M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-03MMagnetic resonance imaging data of animal 14-MI12026-03M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-04MMagnetic resonance imaging data of animal 14-MI12026-04M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-05MMagnetic resonance imaging data of animal 14-MI12026-05M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-06MMagnetic resonance imaging data of animal 14-MI12026-06M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-07MMagnetic resonance imaging data of animal 14-MI12026-07M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-08MMagnetic resonance imaging data of animal 14-MI12026-08M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-09MMagnetic resonance imaging data of animal 14-MI12026-09M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-10MMagnetic resonance imaging data of animal 14-MI12026-10M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-11MMagnetic resonance imaging data of animal 14-MI12026-11M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.14-MI12026-12MMagnetic resonance imaging data of animal 14-MI12026-12M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.15-MI10230-01MMagnetic resonance imaging data of animal 15-MI10230-01M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.15-MI10230-02MMagnetic resonance imaging data of animal 15-MI10230-02M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.15-MI10230-03MMagnetic resonance imaging data of animal 15-MI10230-03M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.15-MI10230-04MMagnetic resonance imaging data of animal 15-MI10230-04M, acquired with a Bruker 7 T magnet, with ParaVision 5.1 software. Each subfolder contains a ParaVisions study. The name of the subfolder refers to the date of data acquisition. An overview of all animals is provided in 'animal_overview.xlsx'.