cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5907484.v1
title: Identification of Anti-Inflammatory and Anti-Hypertensive Drugs as Inhibitors of Bacterial Diguanylate Cyclases

description: Biofilms are widely present in many human chronic infections, often more resistant to treatment with antibiotics. Bacterial diguanylate cyclases (DGCs) synthesize cyclic dimeric guanosine monophosphate (c-di-GMP) from two guanosine-5'-triphosphate (GTP) molecules. c-di-GMP is a central second messenger controlling biofilm formation, turning this class of enzymes an attractive target to prevent and disrupt biofilms of pathogenic bacteria. Here, we apply an in silico ligand- and target-based hybrid method to screen potential DGC inhibitors from an FDA-approved drug databank. Mass spectrometry assays confirmed that seven screened compounds selectively bound to the GTP active site of P. aeruginosa WspR GGDEF domain. Four out of those, including the anti-inflammatory sulfasalazine and the anti-hypertensive eprosartan, inhibited distinct DGCs (P. aeruginosa WspR and E. coli YdeH) in the micromolar range. Sulfasalazine and eprosartan reduced aggregation in solution of E. coli overexpressing WspR or YdeH. Similar anti-aggregation effects were also observed for sulfasalazine-related anti-inflammatory drugs sulfadiazine and sulfathiazole, the latter a previously described anti-biofilm agent. The optimized pharmacokinetic properties and toxicological profiles of the DGC inhibitors could be promising candidates for new anti-microbial agents based on the drug reposition strategy.
DOI: 10.6084/m9.figshare.5907475
title: Policies to support the oil and gas industry in Brazil: a study of public actions to develop the value chain

description: Abstract Because of its strategic importance to the economy and politics, the Oil and Gas Industry played a central role, especially from the 1970s onwards, in an intense involvement of the Brazilian state. In this context, the objective of this article is to analyze the Value Chain Development Policy of the Oil and Natural Gas Industry between 1999 and 2014, especially the Repetro programs, Local Content Policy and the BNDES O&G Program. A qualitative method of “Coherence Postulate”, based on the interrelation of three components, Organizations-Methods-Purposes with specific elements of each policy, is used. Thus, the method is intended to assist in evaluating the consistency between the components and the planned policy results. It is concluded that the policies are inconsistent and do not make it possible to develop public actions and achieve the results required to increase the competitiveness of domestic enterprises.
DOI: 10.6084/m9.figshare.5907475.v1
title: Policies to support the oil and gas industry in Brazil: a study of public actions to develop the value chain

description: Abstract Because of its strategic importance to the economy and politics, the Oil and Gas Industry played a central role, especially from the 1970s onwards, in an intense involvement of the Brazilian state. In this context, the objective of this article is to analyze the Value Chain Development Policy of the Oil and Natural Gas Industry between 1999 and 2014, especially the Repetro programs, Local Content Policy and the BNDES O&G Program. A qualitative method of “Coherence Postulate”, based on the interrelation of three components, Organizations-Methods-Purposes with specific elements of each policy, is used. Thus, the method is intended to assist in evaluating the consistency between the components and the planned policy results. It is concluded that the policies are inconsistent and do not make it possible to develop public actions and achieve the results required to increase the competitiveness of domestic enterprises.
DOI: 10.6084/m9.figshare.5907469
title: Morpho/Proteomic Comparative between High Grade Pleomorphic Sarcoma and Metastasis Diagnosed in an Old Captive Common Hippo

description: Old age is a risk factor for cancer development in humans and animals, and studies have shown that tumors in animals are acceptable models for studying human cancers, considering the similarities between their factors. This work was conducted in a 53-year-old captive female common hippo (Hippopotamus amphibious) with a left leg tumor and metastatic mass. Histopathological and immunohistochemical analyses were carried out with a final diagnosis of a high grade pleomorphic sarcoma. A proteomic study using mass spectrometry was added in order to identify further aspects of the primary tumor and metastasis which could improve our understanding, and each tissue showed a proteomic profile indicative of its pathologic state with significant differences between healthy tissue, primary and metastatic tumors. Low levels of β-actin in primary tumors were identified, and this may be associated with a possible consequence of cytoskeleton dynamic modification. In metastatic tissue, these dynamics may be affected by the presence of HSP chaperone 60.
DOI: 10.6084/m9.figshare.5907469.v1
title: Morpho/Proteomic Comparative between High Grade Pleomorphic Sarcoma and Metastasis Diagnosed in an Old Captive Common Hippo

description: Old age is a risk factor for cancer development in humans and animals, and studies have shown that tumors in animals are acceptable models for studying human cancers, considering the similarities between their factors. This work was conducted in a 53-year-old captive female common hippo (Hippopotamus amphibious) with a left leg tumor and metastatic mass. Histopathological and immunohistochemical analyses were carried out with a final diagnosis of a high grade pleomorphic sarcoma. A proteomic study using mass spectrometry was added in order to identify further aspects of the primary tumor and metastasis which could improve our understanding, and each tissue showed a proteomic profile indicative of its pathologic state with significant differences between healthy tissue, primary and metastatic tumors. Low levels of β-actin in primary tumors were identified, and this may be associated with a possible consequence of cytoskeleton dynamic modification. In metastatic tissue, these dynamics may be affected by the presence of HSP chaperone 60.
DOI: 10.6084/m9.figshare.5907466
title: A Terceirização Precariza as Relações de Trabalho? O Impacto Sobre Acidentes e Doenças

description: In march 2017, a new regulamentation on outsourced contract relations passed presidential sanction. This bill increased the debate over the consequences of that kind of contract. The discussion, however, was not structurated on empirical basis, once it’s difficult to obtain data on brazilian outsourced workers. In this paper we try to fill this gap and test if outsourcing diminishes work safety, affecting the accidents suffered by the workers. We conclude that outsourcing decreases the probability of the worker having an accident, but increase the severity of the accidents. Further estimates suggest that our results were not driven for unobserved individual characteristics.
DOI: 10.6084/m9.figshare.5907466.v1
title: A Terceirização Precariza as Relações de Trabalho? O Impacto Sobre Acidentes e Doenças

description: In march 2017, a new regulamentation on outsourced contract relations passed presidential sanction. This bill increased the debate over the consequences of that kind of contract. The discussion, however, was not structurated on empirical basis, once it’s difficult to obtain data on brazilian outsourced workers. In this paper we try to fill this gap and test if outsourcing diminishes work safety, affecting the accidents suffered by the workers. We conclude that outsourcing decreases the probability of the worker having an accident, but increase the severity of the accidents. Further estimates suggest that our results were not driven for unobserved individual characteristics.
DOI: 10.6084/m9.figshare.5907463
title: Lack of association between rrl and erm(41) mutations and clarithromycin resistance in Mycobacterium abscessus complex

description: BACKGROUND Mycobacterium abscessus complex (MABC) includes species with high resistance rates among mycobacterial pathogens. In fact, MABC infections may not respond to clarithromycin treatment, which has historically been very effective against MABC infection. Molecular markers have been proposed to detect both acquired (rrl polymorphisms) and inducible (erm(41) polymorphisms) clarithromycin resistance in MABC isolates. OBJECTIVES This study aimed to evaluate the susceptibility profile and molecular markers of clarithromycin resistance in MABC. METHODS The clarithromycin susceptibility profile was determined by broth microdilution with reads on days 3, 5, 7 and 14. Mutations in the rrl and erm(41) genes were evaluated by polymerase chain reaction (PCR) using specific primers, followed by sequencing. FINDINGS A total of 14 M. abscessus subsp. abscessus isolates and 28 M. abscessus subsp. massiliense isolates were evaluated, and clarithromycin resistance was observed in all isolates for up to three days of incubation. None of the 42 isolates exhibited a point mutation in the rrl gene, while all the isolates had a T28 polymorphism in the erm(41) gene. Moreover, all 28 M. abscessus subsp. massiliense isolates had a deletion in the erm(41) gene. MAIN CONCLUSIONS While all the MABC isolates exhibited acquired clarithromycin resistance, no isolates exhibited a point mutation in the rrl gene in this study. The M. abscessus subsp. massiliense isolates demonstrated clarithromycin resistance, which is an uncommon phenotype. The molecular data for the rrl and erm(41) genes were not consistent with the phenotypic test results of clarithromycin susceptibility, indicating a lack of correlation between molecular clarithromycin resistance markers for both acquired and inducible resistance.
DOI: 10.6084/m9.figshare.5907463.v1
title: Lack of association between rrl and erm(41) mutations and clarithromycin resistance in Mycobacterium abscessus complex

description: BACKGROUND Mycobacterium abscessus complex (MABC) includes species with high resistance rates among mycobacterial pathogens. In fact, MABC infections may not respond to clarithromycin treatment, which has historically been very effective against MABC infection. Molecular markers have been proposed to detect both acquired (rrl polymorphisms) and inducible (erm(41) polymorphisms) clarithromycin resistance in MABC isolates. OBJECTIVES This study aimed to evaluate the susceptibility profile and molecular markers of clarithromycin resistance in MABC. METHODS The clarithromycin susceptibility profile was determined by broth microdilution with reads on days 3, 5, 7 and 14. Mutations in the rrl and erm(41) genes were evaluated by polymerase chain reaction (PCR) using specific primers, followed by sequencing. FINDINGS A total of 14 M. abscessus subsp. abscessus isolates and 28 M. abscessus subsp. massiliense isolates were evaluated, and clarithromycin resistance was observed in all isolates for up to three days of incubation. None of the 42 isolates exhibited a point mutation in the rrl gene, while all the isolates had a T28 polymorphism in the erm(41) gene. Moreover, all 28 M. abscessus subsp. massiliense isolates had a deletion in the erm(41) gene. MAIN CONCLUSIONS While all the MABC isolates exhibited acquired clarithromycin resistance, no isolates exhibited a point mutation in the rrl gene in this study. The M. abscessus subsp. massiliense isolates demonstrated clarithromycin resistance, which is an uncommon phenotype. The molecular data for the rrl and erm(41) genes were not consistent with the phenotypic test results of clarithromycin susceptibility, indicating a lack of correlation between molecular clarithromycin resistance markers for both acquired and inducible resistance.
DOI: 10.6084/m9.figshare.5907460
title: Improvement of Titanium Corrosion Resistance by Coating with Poly-Caprolactone and Poly-Caprolactone/Titanium Dioxide: Potential Application in Heart Valves

description: Heart diseases affect part of world population and generally involves the functioning of valves. Valves replacement is the most common treatment and the choice between synthetic or natural/biological implants depends on several factors. Synthetic implants have greater durability, whereas biological ones are more biocompatible. This work proposes the use of polymeric coating on titanium metal surface to increase implant biocompatibility. Poly-caprolactone (PCL) has demonstrated greater efficacy for biomedical applications due to its biocompatibility. It can easily form films and coat surfaces. Titanium discs were submitted to alkaline and thermal treatments and coated with 1%PCL and 1%PCL+TiO2. For both conditions, PCL crystals were found in titanium coated surface (SEM and EDX) and X-ray diffractogram confirmed PCL coating. Infrared Spectroscopy spectra showed both PCL and TiO2 characteristic peaks. Moreover, corrosion resistance of coated disc has considerably increased, proving the effectiveness of PCL as coating material and its potential application in cardiac valves.
DOI: 10.6084/m9.figshare.5907460.v1
title: Improvement of Titanium Corrosion Resistance by Coating with Poly-Caprolactone and Poly-Caprolactone/Titanium Dioxide: Potential Application in Heart Valves

description: Heart diseases affect part of world population and generally involves the functioning of valves. Valves replacement is the most common treatment and the choice between synthetic or natural/biological implants depends on several factors. Synthetic implants have greater durability, whereas biological ones are more biocompatible. This work proposes the use of polymeric coating on titanium metal surface to increase implant biocompatibility. Poly-caprolactone (PCL) has demonstrated greater efficacy for biomedical applications due to its biocompatibility. It can easily form films and coat surfaces. Titanium discs were submitted to alkaline and thermal treatments and coated with 1%PCL and 1%PCL+TiO2. For both conditions, PCL crystals were found in titanium coated surface (SEM and EDX) and X-ray diffractogram confirmed PCL coating. Infrared Spectroscopy spectra showed both PCL and TiO2 characteristic peaks. Moreover, corrosion resistance of coated disc has considerably increased, proving the effectiveness of PCL as coating material and its potential application in cardiac valves.
DOI: 10.6084/m9.figshare.5907457
title: Is Composting a Route to Solubilize Low-Grade Phosphate Rocks and Improve MAP-Based Composts?

description: ABSTRACT: In alkalinized and Ca-rich composts, solubilization of apatite from phosphate rocks (PRs) is not guaranteed; however, chelating agents and humified substances produced during composting may alter the soluble contents and P forms of monoammonium phosphate (MAP)-based composts. These effects may depend on the proportions of organic wastes and P source used in the compost piles. The aim of this study was to evaluate the effect of composting chicken manure, coffee husk, and Araxá PR, Bayóvar PR, or MAP in different proportions on the organic matter decomposition, total N, Ca contents, and soluble P fractions in the composts. The treatments consisted of a 3 × 4 × 2 factorial, through the combination of three P sources [Araxá PR, Bayóvar PR, and MAP], with four mixtures in the respective proportions: 25, 40, 50, and 75 % of P source with 37.5, 40, 25, and 12.5 % of chicken manure, and 37.5, 20, 25, and 12.5 % of coffee husk, composted or not for 150 days. The composts with PRs showed greater reductions in total C and water-soluble C and lower dry mass yields than MAP-based composts. The use of MAP in mixtures ensured lower N losses compared to composts formulated with PRs. Regardless of the mixture among chicken manure, coffee husk, and PRs, composting increased the pH and total Ca contents and did not alter the fractions of soluble P in water, in citric acid, and in neutral ammonium citrate plus water in the final PR-based composts. Composting of these mixtures was not an efficient route to solubilize P from Araxá and Bayóvar PRs. Values of pH above 8 and high Ca contents were the main factors explaining the stability and non-solubilization of the apatite of PRs in the composts. Composting with MAP, mixed in different proportions with chicken manure and coffee husk, reduced water-soluble P, maintained the pH of the mixtures in the range of 5 to 7, and enriched the composts with N and P.
DOI: 10.6084/m9.figshare.5907457.v1
title: Is Composting a Route to Solubilize Low-Grade Phosphate Rocks and Improve MAP-Based Composts?

description: ABSTRACT: In alkalinized and Ca-rich composts, solubilization of apatite from phosphate rocks (PRs) is not guaranteed; however, chelating agents and humified substances produced during composting may alter the soluble contents and P forms of monoammonium phosphate (MAP)-based composts. These effects may depend on the proportions of organic wastes and P source used in the compost piles. The aim of this study was to evaluate the effect of composting chicken manure, coffee husk, and Araxá PR, Bayóvar PR, or MAP in different proportions on the organic matter decomposition, total N, Ca contents, and soluble P fractions in the composts. The treatments consisted of a 3 × 4 × 2 factorial, through the combination of three P sources [Araxá PR, Bayóvar PR, and MAP], with four mixtures in the respective proportions: 25, 40, 50, and 75 % of P source with 37.5, 40, 25, and 12.5 % of chicken manure, and 37.5, 20, 25, and 12.5 % of coffee husk, composted or not for 150 days. The composts with PRs showed greater reductions in total C and water-soluble C and lower dry mass yields than MAP-based composts. The use of MAP in mixtures ensured lower N losses compared to composts formulated with PRs. Regardless of the mixture among chicken manure, coffee husk, and PRs, composting increased the pH and total Ca contents and did not alter the fractions of soluble P in water, in citric acid, and in neutral ammonium citrate plus water in the final PR-based composts. Composting of these mixtures was not an efficient route to solubilize P from Araxá and Bayóvar PRs. Values of pH above 8 and high Ca contents were the main factors explaining the stability and non-solubilization of the apatite of PRs in the composts. Composting with MAP, mixed in different proportions with chicken manure and coffee husk, reduced water-soluble P, maintained the pH of the mixtures in the range of 5 to 7, and enriched the composts with N and P.
DOI: 10.6084/m9.figshare.5907454
title: ALTERNATIVE SOURCE APPORTIONMENT IN THE SURROUNDING REGION OF A LARGE STEEL INDUSTRY APPLYING Tillandsia usneoides AS BIOMONITOR

description: From the beginning of its operation, this large steel industrial complex in the Santa Cruz Industrial District, Rio de Janeiro, Brazil, with an annual capacity of 5 million tons, has been at the center of controversy related to its atmospheric emissions. Since the air filter used for its routine air particulate monitoring network is not appropriate for a source apportionment study, biomonitoring was tested as an alternative way to carry out this evaluation. Thus, the bromeliad species Tillandsia usneoides was used as a bioindicator in the Santa Cruz Industrial District, Rio de Janeiro, Brazil. Six samplings were performed over a period of approximately one year. The results showed that the sampling point located inside the industrial complex presented higher elemental concentration values for all samples. Among the quantifiable elements found in the biomonitor samples, iron seems to be the element that best represents the emissions from the steelwork complex, which was corroborated based on the analysis of dust jar samples collected inside the complex area.
DOI: 10.6084/m9.figshare.5907454.v1
title: ALTERNATIVE SOURCE APPORTIONMENT IN THE SURROUNDING REGION OF A LARGE STEEL INDUSTRY APPLYING Tillandsia usneoides AS BIOMONITOR

description: From the beginning of its operation, this large steel industrial complex in the Santa Cruz Industrial District, Rio de Janeiro, Brazil, with an annual capacity of 5 million tons, has been at the center of controversy related to its atmospheric emissions. Since the air filter used for its routine air particulate monitoring network is not appropriate for a source apportionment study, biomonitoring was tested as an alternative way to carry out this evaluation. Thus, the bromeliad species Tillandsia usneoides was used as a bioindicator in the Santa Cruz Industrial District, Rio de Janeiro, Brazil. Six samplings were performed over a period of approximately one year. The results showed that the sampling point located inside the industrial complex presented higher elemental concentration values for all samples. Among the quantifiable elements found in the biomonitor samples, iron seems to be the element that best represents the emissions from the steelwork complex, which was corroborated based on the analysis of dust jar samples collected inside the complex area.
DOI: 10.6084/m9.figshare.5907451
title: Surface Imprinting of Silica Gel by Methyldopa and Its Application in the Solid Phase Extraction Procedure

description: An imprinted amino-functionalized silica gel material was prepared by surface molecular imprinting technique combined with a sol-gel process on the supporter of activated silica gel and used as a solid-phase extraction for the extraction and determination of methyldopa (MTD). Non-imprinted silica adsorbent was synthesized without the addition of MTD using the same process. MTD-imprinted and non-imprinted silica adsorbents were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and the static adsorption experiments. The prepared MTD-imprinted silica sorbent demonstrated high adsorption capacity and significant selectivity for MTD in the presence of competitive substances. The maximum adsorption capacity of surface imprinted and non-imprinted silica adsorbent for MTD were 14565.81 and 2990.56 µg g -1, respectively. The imprinted 3-aminopropyl triethoxysilane (APS) modified silica gel adsorbent provided fast kinetics for the adsorption of MTD. In the operational mode of solid phase extraction (SPE), calibration curve of MTD solution was linear in the range of about 15 to 100 µg mL -1. The limit of detection (LOD) and precision (RSD) during five replicates were 2.31 µg mL -1 and 5.69%, respectively. At last, the MTD-imprinted material was used as SPE adsorbent to determine the MTD in non-spiked and spiked pharmaceutical samples with satisfactory recovery higher than 94%.
DOI: 10.6084/m9.figshare.5907451.v1
title: Surface Imprinting of Silica Gel by Methyldopa and Its Application in the Solid Phase Extraction Procedure

description: An imprinted amino-functionalized silica gel material was prepared by surface molecular imprinting technique combined with a sol-gel process on the supporter of activated silica gel and used as a solid-phase extraction for the extraction and determination of methyldopa (MTD). Non-imprinted silica adsorbent was synthesized without the addition of MTD using the same process. MTD-imprinted and non-imprinted silica adsorbents were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and the static adsorption experiments. The prepared MTD-imprinted silica sorbent demonstrated high adsorption capacity and significant selectivity for MTD in the presence of competitive substances. The maximum adsorption capacity of surface imprinted and non-imprinted silica adsorbent for MTD were 14565.81 and 2990.56 µg g -1, respectively. The imprinted 3-aminopropyl triethoxysilane (APS) modified silica gel adsorbent provided fast kinetics for the adsorption of MTD. In the operational mode of solid phase extraction (SPE), calibration curve of MTD solution was linear in the range of about 15 to 100 µg mL -1. The limit of detection (LOD) and precision (RSD) during five replicates were 2.31 µg mL -1 and 5.69%, respectively. At last, the MTD-imprinted material was used as SPE adsorbent to determine the MTD in non-spiked and spiked pharmaceutical samples with satisfactory recovery higher than 94%.