cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21340615
title: CERRADO ENCLAVE AND THE UPDATE OF THE MAPPING OF LANDSCAPE UNITS IN THE STATE OF RIO GRANDE DO NORTE

description: Abstract The mapping of landscape units is a dynamic process in which constant modifications are needed. Based on this, this article sought to update the mapping of landscape units in the state of Rio Grande do Norte. This was possible due to the combination of field activities and new methodological procedures, with analyzes and refinements of previous mappings conducted by Silva (2018), Bernardino (2019), and Souza (2020). The main result is the update of a mapping that includes the (re-) delimitation and (re-) classification of Morphoclimatic Domains, Natural Regions, and Geocomplexes in the territory of this state. A new unit was identified and inserted: a cerrado enclave, which, until then, had not been described and analyzed as such in the established literature; consequently, one more natural region and two geocomplexes were mapped and delimited. New territorial occurrences of units already identified/classified in the original work were also mapped and, therefore, there was a resizing of landscapes of different taxa, which resulted from these modifications and of the refinement of their limits, due to the greater scalar detail, with the deepening of the research.
DOI: 10.6084/m9.figshare.21340615.v1
title: CERRADO ENCLAVE AND THE UPDATE OF THE MAPPING OF LANDSCAPE UNITS IN THE STATE OF RIO GRANDE DO NORTE

description: Abstract The mapping of landscape units is a dynamic process in which constant modifications are needed. Based on this, this article sought to update the mapping of landscape units in the state of Rio Grande do Norte. This was possible due to the combination of field activities and new methodological procedures, with analyzes and refinements of previous mappings conducted by Silva (2018), Bernardino (2019), and Souza (2020). The main result is the update of a mapping that includes the (re-) delimitation and (re-) classification of Morphoclimatic Domains, Natural Regions, and Geocomplexes in the territory of this state. A new unit was identified and inserted: a cerrado enclave, which, until then, had not been described and analyzed as such in the established literature; consequently, one more natural region and two geocomplexes were mapped and delimited. New territorial occurrences of units already identified/classified in the original work were also mapped and, therefore, there was a resizing of landscapes of different taxa, which resulted from these modifications and of the refinement of their limits, due to the greater scalar detail, with the deepening of the research.
DOI: 10.6084/m9.figshare.21340618
title: Screening of cyanotoxin producing genes in Ecuadorian freshwater systems

description: Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.
DOI: 10.6084/m9.figshare.21340618.v1
title: Screening of cyanotoxin producing genes in Ecuadorian freshwater systems

description: Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.
DOI: 10.6084/m9.figshare.21340621
title: An updated gene regulatory network reconstruction of multidrug-resistant Pseudomonas aeruginosa CCBH4851

description: BACKGROUND Healthcare-associated infections due to multidrug-resistant (MDR) bacteria such as Pseudomonas aeruginosa are significant public health issues worldwide. A system biology approach can help understand bacterial behaviour and provide novel ways to identify potential therapeutic targets and develop new drugs. Gene regulatory networks (GRN) are examples of in silico representation of interaction between regulatory genes and their targets. OBJECTIVES In this work, we update the MDR P. aeruginosa CCBH4851 GRN reconstruction and analyse and discuss its structural properties. METHODS We based this study on the gene orthology inference methodology using the reciprocal best hit method. The P. aeruginosa CCBH4851 genome and GRN, published in 2019, and the P. aeruginosa PAO1 GRN, published in 2020, were used for this update reconstruction process. FINDINGS Our result is a GRN with a greater number of regulatory genes, target genes, and interactions compared to the previous networks, and its structural properties are consistent with the complexity of biological networks and the biological features of P. aeruginosa. MAIN CONCLUSIONS Here, we present the largest and most complete version of P. aeruginosa GRN published to this date, to the best of our knowledge.
DOI: 10.6084/m9.figshare.21340621.v1
title: An updated gene regulatory network reconstruction of multidrug-resistant Pseudomonas aeruginosa CCBH4851

description: BACKGROUND Healthcare-associated infections due to multidrug-resistant (MDR) bacteria such as Pseudomonas aeruginosa are significant public health issues worldwide. A system biology approach can help understand bacterial behaviour and provide novel ways to identify potential therapeutic targets and develop new drugs. Gene regulatory networks (GRN) are examples of in silico representation of interaction between regulatory genes and their targets. OBJECTIVES In this work, we update the MDR P. aeruginosa CCBH4851 GRN reconstruction and analyse and discuss its structural properties. METHODS We based this study on the gene orthology inference methodology using the reciprocal best hit method. The P. aeruginosa CCBH4851 genome and GRN, published in 2019, and the P. aeruginosa PAO1 GRN, published in 2020, were used for this update reconstruction process. FINDINGS Our result is a GRN with a greater number of regulatory genes, target genes, and interactions compared to the previous networks, and its structural properties are consistent with the complexity of biological networks and the biological features of P. aeruginosa. MAIN CONCLUSIONS Here, we present the largest and most complete version of P. aeruginosa GRN published to this date, to the best of our knowledge.
DOI: 10.6084/m9.figshare.21340626
title: MOBILE APPLICATION FOR ADJUSTING AIR-BLAST SPRAYERS IN COFFEE PLANTATION

description: ABSTRACT Mobile application development advances, particularly for smartphones and tablets, have allowed farmers to make decisions more assertively in their agrobusiness management. This article addresses the development and evaluation of an app aimed at people who deal with the pesticide application technology in coffee farming, more specifically, adjustment and calibration of sprayers. This mobile app provides the main data necessary for a correct calibration of air-blast sprayers to apply pesticides in coffee planting. Its functionalities include calculation of the application rate for each situation (L ha-1) based on data obtained in the field, such as canopy volume. The app, called SprayCafé, was developed for the Android platform using the Java programming language in the integrated development environment Android Studio. After the development, the application was evaluated, based on a questionnaire answered by 139 users, who ranked the following requirements: ease of use, loading time, adequacy of screen resolution, data relevance, sequence of information, and applicability, among others. The system proved to be simple and robust; it was thus assessed as adequate to the field and to be of great value for coffee planting, especially because it allows safer and more adequate pesticide application. The graphical user interface is interactive and easy to use.
DOI: 10.6084/m9.figshare.21340626.v1
title: MOBILE APPLICATION FOR ADJUSTING AIR-BLAST SPRAYERS IN COFFEE PLANTATION

description: ABSTRACT Mobile application development advances, particularly for smartphones and tablets, have allowed farmers to make decisions more assertively in their agrobusiness management. This article addresses the development and evaluation of an app aimed at people who deal with the pesticide application technology in coffee farming, more specifically, adjustment and calibration of sprayers. This mobile app provides the main data necessary for a correct calibration of air-blast sprayers to apply pesticides in coffee planting. Its functionalities include calculation of the application rate for each situation (L ha-1) based on data obtained in the field, such as canopy volume. The app, called SprayCafé, was developed for the Android platform using the Java programming language in the integrated development environment Android Studio. After the development, the application was evaluated, based on a questionnaire answered by 139 users, who ranked the following requirements: ease of use, loading time, adequacy of screen resolution, data relevance, sequence of information, and applicability, among others. The system proved to be simple and robust; it was thus assessed as adequate to the field and to be of great value for coffee planting, especially because it allows safer and more adequate pesticide application. The graphical user interface is interactive and easy to use.
DOI: 10.6084/m9.figshare.21340635
title: Methods to quantify Bacillus simplex-based inoculant and its effect as a seed treatment on field-grown corn and soybean in Brazil

description: Abstract: Growth-promoting bacteria in agriculture have become an important tool to improve crop performance and productivity in the face of climate change and deteriorating soil conditions. Bacillus simplex is a recently developed active ingredient for the growth promotion of corn and soybean in Brazil. This study compared three methods to quantify B. simplex colony-forming units in the inoculant product and evaluated the treatment effects of four different concentrations of a B. simplex-based inoculant on corn and soybean root and shoot dry weight, the Normalized Difference Vegetation Index (NDVI), and yield. Field trials were performed at four different locations for each crop, in Mato Grosso do Sul and Paraná for corn, and in Mato Grosso do Sul, Minas Gerais, and Paraná for soybean. The performance of B. simplex was compared to an Azospirillum brasilense-based inoculant, a polymer seed treatment, and untreated controls. The results showed that the official MAPA method for quantifying microbes in inoculants recovered the highest number of B. simplex colonies. However, all three evaluated quantification methods recovered over 100 million colony-forming units per mL (108 CFU.mL-1). The field results showed that the B. simplex inoculant generally increased corn and soybean yields as much or more as the A. brasilense product and that the polymer seed treatment had no impact on yield. The treatment effect on root and shoot weight, and NDVI, was inconsistent. This research shows that B. simplex is quantifiable with three different methods and that it can improve yield in corn and soy. The Bacillus simplex-based inoculant has the potential to become widely used in Brazil.
DOI: 10.6084/m9.figshare.21340635.v1
title: Methods to quantify Bacillus simplex-based inoculant and its effect as a seed treatment on field-grown corn and soybean in Brazil

description: Abstract: Growth-promoting bacteria in agriculture have become an important tool to improve crop performance and productivity in the face of climate change and deteriorating soil conditions. Bacillus simplex is a recently developed active ingredient for the growth promotion of corn and soybean in Brazil. This study compared three methods to quantify B. simplex colony-forming units in the inoculant product and evaluated the treatment effects of four different concentrations of a B. simplex-based inoculant on corn and soybean root and shoot dry weight, the Normalized Difference Vegetation Index (NDVI), and yield. Field trials were performed at four different locations for each crop, in Mato Grosso do Sul and Paraná for corn, and in Mato Grosso do Sul, Minas Gerais, and Paraná for soybean. The performance of B. simplex was compared to an Azospirillum brasilense-based inoculant, a polymer seed treatment, and untreated controls. The results showed that the official MAPA method for quantifying microbes in inoculants recovered the highest number of B. simplex colonies. However, all three evaluated quantification methods recovered over 100 million colony-forming units per mL (108 CFU.mL-1). The field results showed that the B. simplex inoculant generally increased corn and soybean yields as much or more as the A. brasilense product and that the polymer seed treatment had no impact on yield. The treatment effect on root and shoot weight, and NDVI, was inconsistent. This research shows that B. simplex is quantifiable with three different methods and that it can improve yield in corn and soy. The Bacillus simplex-based inoculant has the potential to become widely used in Brazil.
DOI: 10.6084/m9.figshare.21340639
title: Complications of lung transplantation on computed tomography: pictorial essay

description: Abstract Lung transplantation is becoming increasingly more common as an alternative treatment for end-stage lung disease. Despite advances in laboratory testing, surgical technique, and donor/recipient selection, lung transplantation is still associated with significant mortality, due to postoperative complications. This paper consists of a brief review of postoperative complications in lung transplant recipients, illustrating those complications with computed tomography images.
DOI: 10.6084/m9.figshare.21340639.v1
title: Complications of lung transplantation on computed tomography: pictorial essay

description: Abstract Lung transplantation is becoming increasingly more common as an alternative treatment for end-stage lung disease. Despite advances in laboratory testing, surgical technique, and donor/recipient selection, lung transplantation is still associated with significant mortality, due to postoperative complications. This paper consists of a brief review of postoperative complications in lung transplant recipients, illustrating those complications with computed tomography images.
DOI: 10.6084/m9.figshare.21340641
title: STAKEHOLDER MANAGEMENT AND PROJECT MANAGEMENT OFFICE: EFFECT ON PROJECT RESULTS

description: ABSTRACT Managing stakeholders is relevant for project management, as they affect project results. Likewise, the support of a Project Management Office (PMO) improves these results. This study analyzes the positive influence of stakeholder management on project results, specifically on the likelihood of concluding projects within the predicted time and cost, together with the moderating effect of PMOs. A conceptual model was validated through logistic regression, with data collected through a survey responded by 216 experienced professionals studying in graduate programs on project management. Findings indicate that stakeholder management improves project results in terms of time and cost and confirm that PMO enhances the influence of stakeholder management on these results. As its contribution, the study reinforces stakeholder management and PMO as key elements for successful project management. Sampling was sufficient for research replicability but restricted to professionals of project management living in Peru. Thus, future studies could target a broader population.
DOI: 10.6084/m9.figshare.21340641.v1
title: STAKEHOLDER MANAGEMENT AND PROJECT MANAGEMENT OFFICE: EFFECT ON PROJECT RESULTS

description: ABSTRACT Managing stakeholders is relevant for project management, as they affect project results. Likewise, the support of a Project Management Office (PMO) improves these results. This study analyzes the positive influence of stakeholder management on project results, specifically on the likelihood of concluding projects within the predicted time and cost, together with the moderating effect of PMOs. A conceptual model was validated through logistic regression, with data collected through a survey responded by 216 experienced professionals studying in graduate programs on project management. Findings indicate that stakeholder management improves project results in terms of time and cost and confirm that PMO enhances the influence of stakeholder management on these results. As its contribution, the study reinforces stakeholder management and PMO as key elements for successful project management. Sampling was sufficient for research replicability but restricted to professionals of project management living in Peru. Thus, future studies could target a broader population.
DOI: 10.6084/m9.figshare.21310530
title: Canopeo and GreenSeeker applications as tools to support tropical pasture management

description: ABSTRACT: This study determined whether Canopeo and GreenSeeker measurements in Megathyrsus maximus can estimate plant height, dry matter mass, morphological components, and content of crude protein and neutral detergent fiber at different days of growth. Five plots of 5 × 25m M. maximus grass were defined: subplots of 1×1m were evaluated every three days, in which the pasture shows 3, 6, 9, 12, 15, 18, 21, 24, 27, and 30 days of regrowth. The subplot was evaluated for canopy height and mass accumulation. The canopeo index (CI) obtained at a higher height was lower than those obtained at a smaller height. Higher measurement height increased the normalized difference vegetation index (NDVI) relative to 0.10 m. The highest indexes were observed since 18 d of regrowth. Except for the CI evaluated at 0.10 m of height, the indexes were not correlated to the chemical composition of the forage. The CI and NDVI were positively correlated to plant height, dry matter mass, and leaf index, whereas both were negatively correlated with stalk index. Thus, lower evaluation heights for CI and NDVI can be a good predictor of forage height. Values of 0.83 and 85.8 for NDVI and CI, respectively, indicated an appropriate time to start the grazing of M. maximus.
DOI: 10.6084/m9.figshare.21310530.v1
title: Canopeo and GreenSeeker applications as tools to support tropical pasture management

description: ABSTRACT: This study determined whether Canopeo and GreenSeeker measurements in Megathyrsus maximus can estimate plant height, dry matter mass, morphological components, and content of crude protein and neutral detergent fiber at different days of growth. Five plots of 5 × 25m M. maximus grass were defined: subplots of 1×1m were evaluated every three days, in which the pasture shows 3, 6, 9, 12, 15, 18, 21, 24, 27, and 30 days of regrowth. The subplot was evaluated for canopy height and mass accumulation. The canopeo index (CI) obtained at a higher height was lower than those obtained at a smaller height. Higher measurement height increased the normalized difference vegetation index (NDVI) relative to 0.10 m. The highest indexes were observed since 18 d of regrowth. Except for the CI evaluated at 0.10 m of height, the indexes were not correlated to the chemical composition of the forage. The CI and NDVI were positively correlated to plant height, dry matter mass, and leaf index, whereas both were negatively correlated with stalk index. Thus, lower evaluation heights for CI and NDVI can be a good predictor of forage height. Values of 0.83 and 85.8 for NDVI and CI, respectively, indicated an appropriate time to start the grazing of M. maximus.
DOI: 10.6084/m9.figshare.21340644.v1
title: Beyond eNOS: Genetic influence in NO pathway affecting drug response

description: Abstract Nitric Oxide (NO) has important biological functions, and its production may be influenced by genetic polymorphisms. Since NO mediates the drug response, the same genetic polymorphism that alter NO levels may also impact drug therapy. The vast majority of studies in the literature that assess the genetic influence on NO-related drug response focus on NOS3 (which encodes endothelial nitric oxide synthase), however several other proteins are interconnected in the same pathway and may also impact NO availability and drug response. The aim of this study was to review the literature regarding genetic polymorphisms that influence NO in response to pharmacological agents located in genes other than NOS3. Articles were obtained from Pubmed and consisted of 17 manuscripts that assessed polymorphisms of the following targets: Arginases 1 and 2 (ARG1 and ARG2), dimethylarginine dimethylaminohydrolases 1 and 2 (DDAH1 and DDAH2), and vascular endothelial growth factor (VEGF). Here we analyze the main results of these articles, which show promising evidences that may suggest that the NO-driven pharmacological response is affected by more than the eNOS gene. The search for genetic markers may result in better understanding of the variability of drug response and turn pharmacotherapy involving NO safer and more effective.
DOI: 10.6084/m9.figshare.21340644
title: Beyond eNOS: Genetic influence in NO pathway affecting drug response

description: Abstract Nitric Oxide (NO) has important biological functions, and its production may be influenced by genetic polymorphisms. Since NO mediates the drug response, the same genetic polymorphism that alter NO levels may also impact drug therapy. The vast majority of studies in the literature that assess the genetic influence on NO-related drug response focus on NOS3 (which encodes endothelial nitric oxide synthase), however several other proteins are interconnected in the same pathway and may also impact NO availability and drug response. The aim of this study was to review the literature regarding genetic polymorphisms that influence NO in response to pharmacological agents located in genes other than NOS3. Articles were obtained from Pubmed and consisted of 17 manuscripts that assessed polymorphisms of the following targets: Arginases 1 and 2 (ARG1 and ARG2), dimethylarginine dimethylaminohydrolases 1 and 2 (DDAH1 and DDAH2), and vascular endothelial growth factor (VEGF). Here we analyze the main results of these articles, which show promising evidences that may suggest that the NO-driven pharmacological response is affected by more than the eNOS gene. The search for genetic markers may result in better understanding of the variability of drug response and turn pharmacotherapy involving NO safer and more effective.