DOI: 10.22002/bjmkh-tbb88
title: NR1769pKM101+NR1769

description: Skinny E. coli NR1769 with conjugative plasmid plus skinny E. coli NR1769
description: <strong>Tilt Series Date:</strong> 2014-11-25</p>
 
<strong>Data Taken By:</strong> Elitza Tocheva</p>
 
<strong>Species / Specimen:</strong> Escherichia coli</p>
 
<strong>Strain:</strong> NR1769 pKM101</p>
 
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å², defocus: -10.0 μm, magnification: 22500x. </p>
 
<strong>Microscope:</strong> Caltech Polara</p>
 
<strong>Acquisition Software:</strong> UCSFTomo</p>
 
<strong>Upload Method:</strong> reupload_downloaded</p>
 
<strong>Processing Software Used:</strong> IMOD</p>
 
<strong>Collaborators and Roles:</strong> Christian Baron</p>
 
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown to log phase and mixed 1:1 ratio for 30 min to induce conjugation</p>
 
<strong>Sample Preparation:</strong> Vitrobot</p>
 
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/rawdata/</p>
 
4plus7_M13a0003.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/rawdata/4plus7_M13a0003.mrc" > <i class="download icon"></i> Download </a>
</p>
 
 4plus7_M13a0003.rec, Reconstruction (Pixel Size 0.0 nm), 711.2 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/3dimage_49625/4plus7_M13a0003.rec" > <i class="download icon"></i> Download </a>
</p>
 
 slicer000.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12113/slicer000.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer001.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12114/slicer001.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer002.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12115/slicer002.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer003.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12116/slicer003.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer004.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12117/slicer004.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer005.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12118/slicer005.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer006.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12119/slicer006.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer007.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12120/slicer007.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer008.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12121/slicer008.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer009.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12122/slicer009.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer010.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12123/slicer010.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer011.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12124/slicer011.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer012.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12125/slicer012.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer013.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12126/slicer013.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer014.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12127/slicer014.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer015.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12128/slicer015.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer016.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12129/slicer016.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer017.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12130/slicer017.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer018.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12131/slicer018.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer019.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12132/slicer019.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer020.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12133/slicer020.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer021.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12134/slicer021.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer022.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12135/slicer022.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer023.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12136/slicer023.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer024.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12137/slicer024.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer025.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12138/slicer025.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer026.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12139/slicer026.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer027.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12140/slicer027.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer028.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12141/slicer028.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer029.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-14/file_12142/slicer029.tif" > <i class="download icon"></i> Download </a>
</p>