DOI: 10.5061/dryad.9cnp5hqwm
title: Data from: Transplantation of exogenous mitochondria mitigates myocardial dysfunction after cardiac arrest

description: The incidence of post-cardiac arrest myocardial dysfunction (PAMD) is
 high, and there is currently no effective treatment available. This study
 aims to investigate the protective effects of exogenous mitochondrial
 transplantation. Exogenous mitochondrial transplantation can enhance
 myocardial function and improve the survival rate. Mechanistic studies
 suggest that mitochondrial transplantation can limit impairment in
 mitochondrial morphology, augment the activity of mitochondrial complexes
 II and IV, and raise ATP level. As well, mitochondrial therapy ameliorated
 oxidative stress imbalance, reduced myocardial injury, and thus improved
 PAMD after cardiopulmonary resuscitation (CPR). Our study aims to
 investigate the protective effects of exogenous mitochondrial
 transplantation on the myocardium following cardiac arrest. We utilize a
 rat model of asphyxiation-induced cardiac arrest. Once the model is
 established, the rats are humanely euthanized for sample collection or
 survival analysis. 
description: # Data from: Transplantation of exogenous mitochondria mitigates
 myocardial dysfunction after cardiac arrest Dataset DOI:
 [10.5061/dryad.9cnp5hqwm ](10.5061/dryad.9cnp5hqwm) ## Description of the
 data The following files are available: Apoptosis: The level of the
 apoptosis in rat myocardium was measured as described in the Methods
 section: Flow Cytometry. (file format: fcs; file name: Apoptosis)
 mPTP: The level of the mitochondrial permeability transition pore (mPTP)
 in rat myocardium was measured as described in the Methods section:
 Mitochondrial Swelling Assay. (file format: fcs; file name: *mPTP*). ROS:
 The level of mitochondrial reactive oxygen species (ROS) in rat myocardium
 was measured as described in the Methods section: Determination of
 ROS. (file format: fcs; file name: *ROS*) Data: The Excel spreadsheet
 containing the underlying data numerical values that were used to generate
 the Table / Figures in this publication. (file format: xlsx; file name:
 Data) ## File structure Excel file Name: Data The Excel spreadsheet
 containing the underlying data numerical values that were used to generate
 the Table / Figures in this publication.  Folder Name: Flow cytometry
 Content (3 sub-folders):  1\) Sub-folder 1: Apoptosis Sub-sub-folder1:
 Mito Mito1.fcs: The first repeated detection of myocardial apoptosis in
 the Mito group. Mito2.fcs: The second repeated detection of myocardial
 apoptosis in the Mito group. Mito3.fcs: The third repeated detection of
 myocardial apoptosis in the Mito group. Sub-sub-folder2: NS NS1.fcs: The
 first repeated detection of myocardial apoptosis in the NS group. NS2.fcs:
 The second repeated detection of myocardial apoptosis in the NS group.
 NS3.fcs: The third repeated detection of myocardial apoptosis in the NS
 group. Sub-sub-folder3: Sham Sham1.fcs: The first repeated detection of
 myocardial apoptosis in the Sham group. Sham2.fcs: The second repeated
 detection of myocardial apoptosis in the Sham group. Sham3.fcs: The third
 repeated detection of myocardial apoptosis in the Sham group.
 Sub-sub-folder4: Vehicle Vehicle1.fcs: The first repeated detection of
 myocardial apoptosis in the Vehicle group. Vehicle2.fcs: The second
 repeated detection of myocardial apoptosis in the Vehicle group.
 Vehicle3.fcs: The third repeated detection of myocardial apoptosis in the
 Vehicle group. 2\) Sub-folder 2: mPTP Sub-sub-folder1: Mito Calcein
 AM+CoCl2 1.fcs: The first repeated detection of Calcein AM+CoCl2
 fluorescence in the Mito group. Calcein AM+CoCl2 2.fcs: The second
 repeated detection of Calcein AM+CoCl2 fluorescence in the Mito group.
 Calcein AM+CoCl2 3.fcs: The third repeated detection of Calcein AM+CoCl2
 fluorescence in the Mito group. Calcein AM+CoCl2+lonomycin1.fcs: The first
 repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Mito
 group. Calcein AM+CoCl2+lonomycin2.fcs: The second repeated detection of
 Calcein AM+CoCl2+lonomycin fluorescence in the Mito group. Calcein
 AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the Mito group. Calcein AM1.fcs: The
 first repeated detection of Calcein AM fluorescence in the Mito group.
 Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence
 in the Mito group. Calcein AM3.fcs: The third repeated detection of
 Calcein AM fluorescence in the Mito group. Negative1.fcs: The first
 repeated detection of negative fluorescence in the Mito group.
 Negative2.fcs: The second repeated detection of negative fluorescence in
 the Mito group. Negative3.fcs: The third repeated detection of negative
 fluorescence in the Mito group. Sub-sub-folder2: NS Calcein AM+CoCl2
 1.fcs: The first repeated detection of Calcein AM+CoCl2 fluorescence in
 the NS group. Calcein AM+CoCl2 2.fcs: The second repeated detection of
 Calcein AM+CoCl2 fluorescence in the NS group. Calcein AM+CoCl2 3.fcs: The
 third repeated detection of Calcein AM+CoCl2 fluorescence in the NS group.
 Calcein AM+CoCl2+lonomycin1.fcs: The first repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the NS group. Calcein
 AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the NS group. Calcein
 AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the NS group. Calcein AM1.fcs: The
 first repeated detection of Calcein AM fluorescence in the NS group.
 Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence
 in the NS group. Calcein AM3.fcs: The third repeated detection of Calcein
 AM fluorescence in the NS group. Negative1.fcs: The first repeated
 detection of negative fluorescence in the NS group. Negative2.fcs: The
 second repeated detection of negative fluorescence in the NS group.
 Negative3.fcs: The third repeated detection of negative fluorescence in
 the NS group. Sub-sub-folder3: Sham Calcein AM+CoCl2 1.fcs: The first
 repeated detection of Calcein AM+CoCl2 fluorescence in the Sham group.
 Calcein AM+CoCl2 2.fcs: The second repeated detection of Calcein
 AM+CoCl2 fluorescence in the Sham group. Calcein AM+CoCl2 3.fcs: The third
 repeated detection of Calcein AM+CoCl2 fluorescence in the Sham group.
 Calcein AM+CoCl2+lonomycin1.fcs: The first repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the Sham group. Calcein
 AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the Sham group. Calcein
 AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the Sham group. Calcein AM1.fcs: The
 first repeated detection of Calcein AM fluorescence in the Sham group.
 Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence
 in the Sham group. Calcein AM3.fcs: The third repeated detection of
 Calcein AM fluorescence in the Sham group. Negative1.fcs: The first
 repeated detection of negative fluorescence in the Sham group.
 Negative2.fcs: The second repeated detection of negative fluorescence in
 the Sham group. Negative3.fcs: The third repeated detection of negative
 fluorescence in the Sham group. Sub-sub-folder4: Vehicle Calcein AM+CoCl2
 1.fcs: The first repeated detection of Calcein AM+CoCl2 fluorescence in
 the Vehicle group. Calcein AM+CoCl2 2.fcs: The second repeated detection
 of Calcein AM+CoCl2 fluorescence in the Vehicle group. Calcein AM+CoCl2
 3.fcs: The third repeated detection of Calcein AM+CoCl2 fluorescence in
 the Vehicle group. Calcein AM+CoCl2+lonomycin1.fcs: The first repeated
 detection of Calcein AM+CoCl2+lonomycin fluorescence in the Vehicle group.
 Calcein AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the Vehicle group. Calcein
 AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein
 AM+CoCl2+lonomycin fluorescence in the Vehicle group. Calcein AM1.fcs: The
 first repeated detection of Calcein AM fluorescence in the Vehicle group.
 Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence
 in the Vehicle group. Calcein AM3.fcs: The third repeated detection of
 Calcein AM fluorescence in the Vehicle group. Negative1.fcs: The first
 repeated detection of negative fluorescence in the Vehicle group.
 Negative2.fcs: The second repeated detection of negative fluorescence in
 the Vehicle group. Negative3.fcs: The third repeated detection of negative
 fluorescence in the Vehicle group. 3\) Sub-folder 3: ROS Sub-sub-folder1:
 Mito Mito1.fcs: The first repeated detection of mitochondrial ROS in the
 Mito group. Mito2.fcs: The second repeated detection of mitochondrial ROS
 in the Mito group. Mito3.fcs: The third repeated detection of
 mitochondrial ROS in the Mito group. Sub-sub-folder2: Negative control
 Tube1.fcs: The negative fluorescence detection.  Sub-sub-folder3: NS
 NS1.fcs: The first repeated detection of mitochondrial ROS in the NS
 group. NS2.fcs: The second repeated detection of mitochondrial ROS in the
 NS group. NS3.fcs: The third repeated detection of mitochondrial ROS in
 the NS group. Sub-sub-folder4: Sham Sham1.fcs: The first repeated
 detection of mitochondrial ROS in the Sham group. Sham2.fcs: The second
 repeated detection of mitochondrial ROS in the Sham group. Sham3.fcs: The
 third repeated detection of mitochondrial ROS in the Sham group.
 Sub-sub-folder5: Vehicle Vehicle1.fcs: The first repeated detection of
 mitochondrial ROS in the Vehicle group. Vehicle2.fcs: The second repeated
 detection of mitochondrial ROS in the Vehicle group. Vehicle3.fcs: The
 third repeated detection of mitochondrial ROS in the Vehicle group. Links
 to other publicly accessible locations of the data: N/a **Code/Software**
 The FCS file can be opened and analyzed using various bioinformatics tools
 and software. One of the most common options is FlowJo.  Other software
 options include FCS Express, Kaluza, WinList, and several others.