DOI: 10.5061/dryad.h18931ztf
title: Data from: Can females differentially allocate resources to offspring sired by different males?

description: The viviparity-driven conflict hypothesis postulates that the evolution of
 matrotrophy (postfertilization maternal provisioning) will result in a
 shift from a pre- to postcopulatory mate choice and thus accelerate the
 evolution of postcopulatory reproductive isolation. Here, we perform
 artificial insemination experiments on Heterandria formosa, a matrotrophic
 poeciliid fish, to probe for evidence of postcopulatory female choice. We
 established laboratory populations from Wacissa River (WR) and Lake
 Jackson (LJ). The WR females normally produce larger offspring than the LJ
 females. We artificially inseminated females with sperm from each
 population or from both populations simultaneously. When LJ females were
 inseminated with sperm from WR and LJ males, they allocated fewer
 resources to WR-sired offspring than when they were inseminated with WR
 sperm alone. The LJ females carrying developing offspring sired by males
 from different populations were thus able to discriminate against
 non-resident males when allocating resources to developing young. The WR
 females, which normally produce larger offspring than LJ females, did not
 discriminate among males from different localities. These findings provide
 insights into the ability of females from one population to exercise a
 form of postcopulatory mate selection.
description: We inseminated female <em>Heterandria formosa</em>
 with spermatozeugmata from either two or four males, comprising four
 treatments: (A) female × 2 WR males; (B) female × 2 LJ males; (C)
 female × 1 WR male + 1 LJ male; and (D) female × 2 WR males + 2 LJ males.
 These treatments were later combined into ‘Single’ male population and
 ‘Mixed’ male population insemination treatments. These data include
 offspring weights and genotypes from fish born in this experiment. The
 data associated with each offspring included: days since insemination,
 number of broods per female, and number of offspring per brood. Any
 offspring born within a 48 h interval were counted towards a single brood,
 because there is typically a 3- to 5-day interval between broods, and two
 broods born within 48 h is highly unlikely.
description: # Can females differentially allocate resources to offspring sired by
 different males?
 [https://doi.org/10.5061/dryad.h18931ztf](https://doi.org/10.5061/dryad.h18931ztf) We inseminated female *Heterandria formosa* with spermatozeugmata from either two or four males, comprising four treatments: (A) female × 2 WR males; (B) female × 2 LJ males; (C) female × 1 WR male + 1 LJ male; and (D) female × 2 WR males + 2 LJ males. These treatments were later combined into ‘Single’ male population and ‘Mixed’ male population insemination treatments. These data include offspring weights and genotypes from fish born in this experiment. The data associated with each offspring included: days since insemination, number of broods per female, and number of offspring per brood. Any offspring born within a 48 h interval were counted towards a single brood, because there is typically a 3- to 5-day interval between broods, and two broods born within 48 h is highly unlikely. ## Description of the data and file structure Here is a key to the .csv file (mg = milligrams, g=grams): ofid = Offspring identification number offscount = the number of offspring represented in the row (for summing purposes) ETOHWeight = ethanol weight in mg wet_wt = wet weight in g wet_wt_mg = wet weight in mg dam_id = the dam's identification number dam_pop = the originating population (Wacissa River/WR or Lake Jackson/LJ) of the dam Treatment = (A) female × 2 WR males; (B) female × 2 LJ males; (C) female × 1 WR male + 1 LJ male; and (D) female × 2 WR males + 2 LJ males MomWTI = dam weight at the beginning of the experiment brood = the brood from which the offspring originated sire_id = the sire's identification number as determined by genotyping treat_type = S for single with two males from the same population or M for Mixed insemination with one male from each population sire_pop = the originating population (Wacissa River/WR or Lake Jackson/LJ) of the sire determined by genotyping SireTLI = total length for the sire as determined by genotyping Note: cells containing NA mean there is no relevant or recorded data available for that cell.  ## Code/Software An annotated R script is included. At the time of publication, version R4.2.2 was used. All loaded packages are included in the script, as well as here: tidyverse lme4 car emmeans plotrix modelbased ggpubr