cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14291196.v1
title: The importance of Serra do Mar State Park for liverworts conservation in the Atlantic Rainforest

description: Abstract Serra do Mar State Park (PESM) is located in southeastern São Paulo state, Brazil, and is the largest Atlantic Rainforest conservation area in the country. The park is divided into 10 nuclei, and the Itutinga-Pilões nucleus (NIP) was selected for study since no survey of liverworts species richness had yet been conducted there. The floristic similarities between the NIP and different areas of Atlantic Rainforest in São Paulo state were also assessed. One hundred and eighty liverworts species were identified in the three vegetation types (lowland, submontane, and montane ombrophilous forests) encountered in the NIP, distributed among 62 genera and 21 families. Lejeuneaceae showed the highest species richness with 80 species. The high number of epiphyllous species found in the study area (26% of all species) was notable. In terms of their worldwide distributions, 57% of the species are Neotropical. Clustering analysis showed that the areas of the PESM and Jureia-Itatins Ecological Station were grouped together with the highest similarity values. The liverworts flora of the NIP demonstrates the importance of that nucleus for the conservation of liverworts diversity in the Atlantic Rainforest of the São Paulo state, as well as in Brazil.
DOI: 10.6084/m9.figshare.14291195.v1
title: Estimation of leaf area of Mesosphaerum suaveolens from allometric relations

description: Abstract The determination of leaf area is of fundamental importance in ecophysiological studies that seek to understand the plant-environment relationship. The objective of this research was to determine an allometric equation to estimate the leaf area of Mesosphaerum suaveolens from linear measures of leaf blade (length and/or width). There were collected 900 random leaf limbs of different shapes and sizes, in an anthropic area of caatinga, located in the municipality of Santa Luzia, state of Paraíba, Northeast of Brazil. For the construction of the allometric equations the following regression models were used: linear, linear without intercept, quadratic, cubic, power, and exponential. The linear model without intercept using the product between length and width (C.L) was the most recommended to estimate the leaf area of M. suaveolens, with greater coefficient of determination (R2) and Willmott concordance index (d), lesser mean absolute error (MAE), Akaike information criterion (AIC) and root-mean-square error (RQME), and the closer the BIAS ratio is to zero (R2 = 0.9971; d = 0.9989; MAE = 0.422; AIC = 2094.69; RQME = 0.770; BIAS = -0.0105). The leaf area of M. suaveolens can be estimated satisfactorily by equation AF = 0.6787*(C.L).
DOI: 10.6084/m9.figshare.14291190.v1
title: Bromeliaceae in Atlantic Forest fragments of Juiz de Fora, Minas Gerais, Brazil

description: Abstract The vegetation fragments of the municipality of Juiz de Fora, in the Zona da Mata de Minas Gerais region, are part of the Atlantic Forest Domain, and considered priority for the conservation of the flora in the state. The present work presents a taxonomic study of the family Bromeliaceae in the forest fragments of the municipality. Bromeliaceae is represented in this region by 10 genera and 23 species. Tillandsia is the most representative genus, followed by Aechmea and Billbergia. We also report the first occurrence of Vriesea grandiflora for the Minas Gerais state. Included in this study are a key identification, descriptions, illustrations, taxonomic comments, as well as information on the geographic distribution and conservation status of the species.
DOI: 10.6084/m9.figshare.14291189.v1
title: Chrysobalanaceae in the East part of the Brazilian Northeastern

description: Abstract This study provides descriptions, identification keys, distribution maps, illustrations, and comments about the Chrysobalanaceae species occurring in the East part of the Brazilian Northeastern, which includes the states of Alagoas, Ceará, Paraíba, Pernambuco, and Rio Grande do Norte. Twenty-six species in eight genera were found: Hirtella (eight spp.), Couepia (six spp.), Leptobalanus and Parinari (both with three spp.), Licania and Moquilea (both with two species), and Chrysobalanus and Microdesmia (both with one specie). Hirtella sprucei and Moquilea silvatica are new records for the area and although most species are widely distributed, Couepia impressa, C. pernambucensis, C. rufa, Parinari littoralis, Hirtella sprucei, H. insignis and H. santosii are restricted to the Atlantic Forest, the latter two being considered as Endangered.
DOI: 10.6084/m9.figshare.14291187.v1
title: Piperaceae of Brazilian northeast II: Alagoas state

description: Abstract This study includes the taxonomic treatment of the Piperaceae species present in the Alagoas state, aiming to increase knowledge about the Alagoas flora and continuing the studies with the family in the Brazilian northeast. Thirty species of the family were recorded, distributed in the genus Peperomia (9 spp.) and Piper (21 spp.), of which eight are for the first time reported for the Alagoas state (3 and 5 spp. respectively). Although most of the taxa occur in forested areas, some of them can be found in restinga, brejo de altitude, agreste, cerrado and caatinga. Identification key for the occurring taxa, descriptions for the analyzed species, besides comments about geographical distribution, habitat, biological activity and illustrations of those poorly known, are provided.
DOI: 10.6084/m9.figshare.14291184.v1
title: Adaptive leaf strategies of Miconia nervosa (Melastomataceae) in Amazon of Mato Grosso state

description: Abstract The phenotype plasticity usually observed in morphological and anatomical leaf traits may be influenced by several factors, such as luminosity, soil nutrient availability, humidity and herbivory, among others. We tested leaf plasticity in Miconia nervosa (Melastomataceae) in a forest fragment in Southern Amazonia, Mato Grosso State - Brazil, seeking to verify morpho-anatomical strategies of light acclimation. We collected fully expanded leaves of 15 individuals exposed directly to sunlight, on a lake edge, and 15 under canopy shading. We evaluated quantitatively eight morphological variables and six anatomical variables, of which six and four, respectively, differed significantly between sun and shade leaves. Morphologically, sun leaves showed larger leaf and petiole sizes and anatomically, epidermal cells with slightly thickened walls, higher stomatal index and density. Shade leaves showed significantly thicker epidermis on both leaf faces. Leaves of M. nervosa exhibited medium to high degree of plasticity in morpho-anatomical traits, according to the plasticity index, with mixed characteristics of sun and shade environments. We suggest that the distribution of M. nervosa individuals in the forest is related to the availability of light and soil moisture.
DOI: 10.6084/m9.figshare.14291182.v1
title: Protocol for asymbiotic germination and initial protocorm development of Brazilian Cerrado native orchids

description: Abstract Due to the extractivism Orchidaceae presents high vulnerability, needing measures for the conservation of its species. The aim of this study was to determine a protocol for germination and initial protocorm development of Cattleya nobilior, Cattleya lundii and Brassavola tuberculata for seed disinfestation and the use of culture media. Seeds were disinfested in 0.8% NaClO solution by 5 min or 15 min, receiving or not triple-wash. They were then sowing in MS, 1/2 MS, K or VW culture media. Subsequently, they were transferred to growth room with controlled temperature and photoperiod. The percentage of germination and initial development of the species studied were evaluated 45 days after sowing. For all species, the seeds disinfested by 5 min and submitted to the triple-wash showed higher germination percentage. Thus, is recommended the use of the 5 min disinfestation time, followed by the practice of triple-wash and sowing in 1/2 MS medium, for the germination and in vitro initial establishment of C. nobilior and B. tuberculata (%G = 100 and 72.8% and seedlings in stages 3 and 2, respectively). For C. lundii, sowing should be done in KC medium, with 5 min disinfestation and triple-wash (%G = 95.8% and seedlings in stage 2).
DOI: 10.6084/m9.figshare.14291180.v1
title: Flora of Espírito Santo: Simaroubaceae

description: Abstract We present the taxonomic treatment of Simaroubaceae species occurring in Espírito Santo state, Brazil. This study is the result of the combination of fieldwork and analysis of specimens deposited in herbaria that house relevant collections of the Espírito Santo. The family is represented in the state by six species belonging to three genera: Homalolepis (4 spp.), Picrasma (1 sp.) and Simarouba (1 sp.). All species are forest trees, recorded in submontane dense ombrophilous forest, tabuleiro forest and restinga forest. The treatment includes identification keys, detailed morphological descriptions, illustrations, photographs, and comments on taxonomy, distribution, habitat and phenology of each species.
DOI: 10.6084/m9.figshare.14291178.v1
title: Morpho-physiological classification of seeds and morphology of fruits and seedlings of Richeria grandis

description: Abstract The objective was to describe and characterize the fruit morphology, seeds, seedlings and germination of Richeria grandis. Mature fruits were collected from matrix trees located in gallery forests. After, morphological descriptions were performed. Biometric measurement of fruits and seeds were carried out. A total of 650 seeds were randomly separated, with 20% being used for germination and humidity testing on the same day of collection and the others stored for 5, 10, 15 and 20 days. The germination test was performed in a gerbox, conducted in a laboratory chamber of the type B.O.D (biochemical oxygen demand) with a photoperiod of 12h and a temperature of 25 ºC ± 1 ºC. Periodic observations were made to monitor the development of the seedlings until the first germinated seeds reached the full seedling stage. The fruit of R. grandis is a simple, dry, capsule type septifraga, dehiscent and schizocarpic, with average length of 16.24 mm and an average width of 8.57 mm. The seed is an ellipsoid shape, containing a sarcotesta and has an average length of 7.40 mm and an average width of 4.57 mm. R. grandis presents recalcitrant seeds, which emerge in a Phanero-epigene-foliaceous manner. The radicle breaks the integument on the second day after sowing, the collection is evidenced on the 10th day, separating the primary root from the hypocotyl. At day 16, the cotyledons expand from the forehead, positioned at a 90º angle to the hypocotyl. The protophyll and the epicotyl appear only after the 50th day, characterizing the complete seedling.
DOI: 10.6084/m9.figshare.14291175.v1
title: Leaf anatomy of Rubiaceae species in a semiarid area of Brazil

description: Abstract Considering the importance of anatomical characters for delimiting Rubiaceae subgroups, the difficulties involved in identifying certain taxa and the lack of studies on that group in the semiarid region, this work aimed to characterize the anatomy of the leaves of 15 species belonging to the genera: Borreria (2), Cordiera (1), Eumachia (1), Hexasepalum (4), Mitracarpus (4), Richardia (1), Staelia (1), and Tocoyena (1) collected in the Serra Branca/Raso da Catarina Environmental Protection Area (Jeremoabo-BA, Brazil), and describe useful elements supporting the group’s taxonomy. Variations were found in the shapes and contours of epidermal cells, the presence/absence and types of trichomes, mesophyll type, stomatal type and position, subsidiary cell shapes, vascular system organization, and the occurrence of collector cells and twinned stomata. The results also demonstrated that the anatomical characteristics, when considered together, represent good taxonomic tools for separating the genera and species of Rubiaceae.
DOI: 10.6084/m9.figshare.14291172.v1
title: Technological properties of woods used in boat’s production in the Southeast of Pará, Brazil

description: Abstract The aim of this study was to identify, anatomically characterize, and determine some of the physical properties of wood species that are used for boat production in the southeast region of the state of Pará in Brazil. The samples were collected during visits to shipyards in the region, then it was identified at the specie level and technologically characterized. Eight species belonging to four distinct botanical families were identified. All identified species had presented some type of deposition in its vessels and 87% of them had had medium density (0.40 to 0.75 g.cm-3). The species with higher frequency of occurrence and that had more parts used for boat production were Caryocar villosum and Apuleia leiocarpa. It was observed two different group arrangements of species, “piquiá” (Caryocar villosum and Alexa grandiflora) and “tatajuba” (Bagassa guianensis and Enterolobium schomburgkii). It was also identified the illegal commercialization of “castanheira’s” wood (Bertholletia excelsa). The results can indicate anatomical and physical similarities in woods used in boat production and can contribute as a component of a database in wood anatomy of specie used for boat production in the Amazon region, besides to subside practices that contribute to an effective supervision of the logging market in the region.
DOI: 10.6084/m9.figshare.14291171.v1
title: Montane seasonal wetlands: an inventory of its associated flora in Parque Estadual do Ibitipoca, southeast Brazil

description: Abstract The aim of the present study was to perform the floristic survey of the wetlands of the Parque Estadual do Ibitipoca (PEIB), comparing them regarding richness, floristic composition and life forms, based on monthly expeditions to collect vascular plants. Eighty-one (81) species were found in the moist grassland, most of them being endemic to Brazil and included in 55 genera and 21 families. The most representative families were Asteraceae (22 spp.), Melastomataceae (nine spp.), Xyridaceae (seven spp.), Poaceae and Orchidaceae (six spp. each), totalizing 62% of the sampled species. Twenty-seven (27) species were found in the seasonal pond, distributed in 23 genera and 11 families. Families with the highest specific richness were Poaceae (eight spp.), Cyperaceae and Asteraceae (five spp. each), totalizing 67% of the sampled species. There was a predominance of hemicryptophyte life form (nearly 43% of the moist grasslands and 63% of the seasonal pond) in both areas. The floristic analysis results showed great importance to conservation with 22% of species under some threatened category, 12 species endemics to Minas Gerais state and one new species of Cyperaceae. Furthermore, only five species occur in both wetland types (5.1% of the species richness).
DOI: 10.6084/m9.figshare.14291168.v1
title: Trichocline minuana (Compositae, Mutisieae), a new species endemic to the Pampas in Southern Brazil and Uruguay

description: Abstract A new species of the genus Trichocline is described. Trichocline minuana is restricted to the state of Rio Grande do Sul, Brazil and northern Uruguay, where is found in lowland pampean grasslands, generally associated to rocky and dry soils. The main characters that distinguish this species from the other sympatric and morphologically close species T. humilis and T. incana are the presence of a small scape and strongly pinnatisect leaves that have lobes with acute apex, and a large, lanceolate terminal lobe. This study provides a detailed description of the species, information about distribution, conservation status assessment and information about its ecological aspects.
DOI: 10.6084/m9.figshare.14291166.v1
title: Reproductive biology and pollination of Palicourea crocea (Rubiaceae), the distylous and ornithophilous specie in the Cerrado of Goiás, Brazil

description: Abstract The heterostyly is a floral polimorphysm genetically controlled where populations of plants present two or three morphs with reproductive structures in reciprocal heights among the morphs. In natural populations, a balanced ratio between morphs (isoplethy) is expected. The objective of the study was to characterize the breeding system, the floral biology, the characteristics of heterostyly, including reciprocity of reproductive parts, the phenology and pollination in Palicourea crocea, a shrub species of the understory of forest formations of Cerrado. For such measures were floral structures, held controlled crossings, observed flower visitors, in addition to phenological monitoring. Palicourea crocea is proved to be a typical distylous species, self-incompatible, with high levels of reciprocal herkogamy between the morphs. Preferentially pollinated by hummingbirds, followed by moths and bees. There was a higher fruit formation in cross-pollination intermorph (&gt; 50%) and lower fruit formation intramorph pollination (&lt; 20%), and self-pollination (&lt; 13%). These data were confirmed by the not growth of pollen tubes in self-pollination and pollinated intramorph pistils. Flowering is annual and occurs between the months of November and February, with peak flowering in December and January. Reproductive data evaluated to show similar to other species of Rubiaceae of the understory of forest formations of Cerrado.
DOI: 10.6084/m9.figshare.14291165.v1
title: An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

description: Abstract Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome. The methods tested consisted of: (i) TRIzol® reagent, (ii) TRIzol® reagent with modifications, (iii) CTAB buffer, and (iv) Modified CTAB buffer, initially for leaf samples of Xylopia aromatica and Piper arboreum. Later the procedure with the best results was used to obtain purified RNA from 17 other native species. Based on A260/A280 absorbance ratio the Modified CTAB method was the best for total RNA extraction for those woody species. Ten out of eleven species tested through RT-PCR generated fragments of the expected size from the total RNA extracted by the selected method, confirming it as the best option to obtain high-quality RNA for molecular analyses and for use in the detection of viruses infecting these tree species.
DOI: 10.6084/m9.figshare.14291163.v1
title: Redescription and extended geographical distribution of Peltaea chiquitana (Malvaceae, Malvoideae)

description: Abstract Peltaea chiquitana has been a name of dubious identity due to the incomplete original description and original materials lacking fruits. Recent herbaria and field work studies have uncovered new data for this species. Thus, we present a redescription of P. chiquitana, including, for the first time, details of column, fruit, and seed morphology. An extended geographical distribution of P. chiquitana is presented, based on the first records of the species to Brazil. Furthermore, line drawings, scanning electronic microscope photographs, a distribution map, and a conservation assessment are presented here.
DOI: 10.6084/m9.figshare.14291162.v1
title: The genus Cladonia (Lichenized Ascomycota) from Restinga Vegetation of Espírito Santo state, Brazil: Supergroups Crustaceae and Perviae

description: Abstract In this paper we recognize a total of 11 species of Cladonia, five belonging to Supergroup Crustaceae and six to Supergroup Perviae, occurring in the Restinga vegetation of Espírito Santo state, Brazil. We confirm the occurrence of C. sprucei and C. rangiferina to Espírito Santo state Restinga, being their most austral record in Brazil so far. Four species are new records to the state: C. consimilis, C. dendroides, C. salzmannii, and C. sphacelata. Here we expand the number of known species of Cladonia of Supergroup Perviae from three to five, and of Supergroup Crustaceae from three to six in the studied environment. An identification key, comments and illustrations are provided.
DOI: 10.6084/m9.figshare.14291160.v1
title: The tribe Bignonieae (Bignoniaceae) in the Itatiaia National Park, Southeastern Brazil

description: Abstract This study consists of a floristic inventory of the species of Bignonieae (Bignoniaceae) recorded for the Itatiaia National Park, Rio de Janeiro, Brazil. The tribe is represented in the region by 12 genera and 18 species: Fridericia (4 spp.), Adenocalymma (2 spp.), Anemopaegma (2 spp.), Dolichandra (2 spp.), Amphilophium (1 sp.), Bignonia (1 sp.), Callichlamys (1 sp.), Lundia (1 sp.), Mansoa (1 sp.), Pleonotoma (1 sp.), Pyrostegia (1 sp.), and Xylophragma (1 sp.). We present keys for the identification of genera and species, descriptions, taxonomic comments and illustrations. Four species are recorded for region for the first time, i.e., Anemopaegma prostratum, Callichlamys latifolia, Dolichandra unguiculata, and Pleonotoma tetraquetra. We compare the floristic similarity among the Bignonieae from the Itatiaia National Park and surrounding areas using Jaccard’s coefficient of similarity and a clustering analysis based on an UPGMA algorithm. The flora of Bignonieae from Itatiaia is more similar to areas of the Atlantic rainforest, such as Picinguaba and Rio Doce, than to areas of Cerrado, as the Serra do Cipó and Grão Mogol. These results suggest that vegetation type is more important than geographic proximity in the composition of the Bignonieae from Itatiaia.
DOI: 10.6084/m9.figshare.14291159.v1
title: The genus Gongora (Orchidaceae, Stanhopeinae) from state of Mato Grosso, Brazil

description: Abstract The taxonomic study of Gongora for Mato Grosso state is presented here. The only species found was Gongora nigrita, a yet unpublished record for the central-west region of Brazil and for Mato Grosso, representing a considerable extension of the austral limits of the species. G. nigrita was found in four municipalities, both in the Juruena and Teles Pires river basins, within the Amazon Rainforest biome. A wide variation in flower colour, ranging from brown to yellow with brown spots was recorded. Flower scent resembles indian cloves, and an analysis of the essence revealed eugenol as the predominant chemical substance. Description, taxonomic and ecological comments, distribution and illustrations are provided.
DOI: 10.6084/m9.figshare.14291158.v1
title: Navicula (Naviculaceae) in the periphyton of streams and new occurrences for Goiás state

description: Abstract This study aimed to describe and inventory the species of Navicula present in the periphyton of the Goiás state streams, and to contribute to state records with the identification of new taxonomic characteristics. Samples were collected from nine lotic environments, where the periphytic material was removed from submerged Gramineae and stones. Afterwards, to facilitate the observation, identification, and description of specimens, slides were prepared from oxidized material. As a result, ten species were identified, four of which were newly recorded in the state: Navicula leptostriata, Navicula symmetrica, Navicula neomundana, Jarlman & and Navicula parablis. The most common taxon identified in this study was Navicula leptostriata, followed by Navicula parablis.