cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19923098
title: Calcium supplementation in nutrient solutions to mitigate salt stress in fertigated okra

description: ABSTRACT The objective of this study was to evaluate the effect of calcium supplementation on saline water as a minimizing agent of saline stress in okra cultivars. The experiment was carried out in a greenhouse in Mossoró-RN using a completely randomized experimental design in a 2x5 factorial scheme with four replications. Two okra cultivars (Santa Cruz 47 and Valença) were tested fertigated with five fertigation managements [F1= irrigation with non-saline water (0.5 dS m-1) and fertigation with a standard dose of calcium (4,733.8 mg plant-1); F2, F3, F4 and F5= irrigation with saline water (3.5 dS m-1) and fertigation with increasing doses of calcium (4,733.8; 7,100.7; 9,467.7 and 11,834.5 mg plant-1, respectively)]. Plants were collected at 120 days after emergence and evaluated for growth variables (plant height, leaf number, stem diameter, leaf dry mass, stem, fruit and total) and yield (fruit number, average length of fruits, average diameter and yield). Cultivar Santa Cruz 47 presented the highest vegetative development but cultivar Valença was earlier and presented the largest number of harvests (19), allowing more harvested fruits (27 fruits per plant) and the highest yield (659 g plant-1). The cultivars were not affected by the saline level and showed little response to extra calcium fertilization.
DOI: 10.6084/m9.figshare.19923098.v1
title: Calcium supplementation in nutrient solutions to mitigate salt stress in fertigated okra

description: ABSTRACT The objective of this study was to evaluate the effect of calcium supplementation on saline water as a minimizing agent of saline stress in okra cultivars. The experiment was carried out in a greenhouse in Mossoró-RN using a completely randomized experimental design in a 2x5 factorial scheme with four replications. Two okra cultivars (Santa Cruz 47 and Valença) were tested fertigated with five fertigation managements [F1= irrigation with non-saline water (0.5 dS m-1) and fertigation with a standard dose of calcium (4,733.8 mg plant-1); F2, F3, F4 and F5= irrigation with saline water (3.5 dS m-1) and fertigation with increasing doses of calcium (4,733.8; 7,100.7; 9,467.7 and 11,834.5 mg plant-1, respectively)]. Plants were collected at 120 days after emergence and evaluated for growth variables (plant height, leaf number, stem diameter, leaf dry mass, stem, fruit and total) and yield (fruit number, average length of fruits, average diameter and yield). Cultivar Santa Cruz 47 presented the highest vegetative development but cultivar Valença was earlier and presented the largest number of harvests (19), allowing more harvested fruits (27 fruits per plant) and the highest yield (659 g plant-1). The cultivars were not affected by the saline level and showed little response to extra calcium fertilization.
DOI: 10.6084/m9.figshare.19918837
title: stickels2021highly_Slide-seqV2_Mouse_Cerebellum

description: stickels2021highly_Slide-seqV2_Mouse_Cerebellum_SCP948.h5ad
DOI: 10.6084/m9.figshare.19918861
title: stickels2021highly_Slide-seqV2_Mouse_Hippocampus

description: stickels2021highly_Slide-seqV2_Mouse_Hippocampus(2 files)
DOI: 10.6084/m9.figshare.19918867
title: stickels2021highly_Slide-seqV2_Mouse_SomatosensoryCortex

description: stickels2021highly_Slide-seqV2_Mouse_SomatosensoryCortex_Puck_200306_03.h5ad
DOI: 10.6084/m9.figshare.19923103
title: Biodegradable mulch controls weeds and increases water use efficiency in lettuce crops

description: ABSTRACT Vegetable cultivation requires high water use and weed control. Soil cover using recycled paper, can be an alternative to polyethylene film to reduce weed incidence, soil temperature and increase water use efficiency beyond reduces costs and environmental pollutions. The objective of this study was to evaluate the use of biodegradable mulch in weed management and water use efficiency (WUE) in lettuce crop. The treatments were composed of brown recycled paper (RP), black polyethylene film (PF) and soil without cover with weed removal (WR) and without weed removal (WW). RP and PF were efficient to control weeds. The soil temperature with RP was 8.2 and 2.1ºC lower than with PF and WR, respectively. The lettuce yield with RP was 14.5 and 28.3% higher than WR, and with PF, respectively. The water volume applied with RP was 26.5% lower, and WUE was 55.6% higher compared to WR. Soil cover with recycled paper controlled weeds, reduced soil temperature and water consumption and increased yield and water use efficiency in lettuce crop.
DOI: 10.6084/m9.figshare.19918867.v1
title: stickels2021highly_Slide-seqV2_Mouse_SomatosensoryCortex

description: stickels2021highly_Slide-seqV2_Mouse_SomatosensoryCortex_Puck_200306_03.h5ad
DOI: 10.6084/m9.figshare.19923103.v1
title: Biodegradable mulch controls weeds and increases water use efficiency in lettuce crops

description: ABSTRACT Vegetable cultivation requires high water use and weed control. Soil cover using recycled paper, can be an alternative to polyethylene film to reduce weed incidence, soil temperature and increase water use efficiency beyond reduces costs and environmental pollutions. The objective of this study was to evaluate the use of biodegradable mulch in weed management and water use efficiency (WUE) in lettuce crop. The treatments were composed of brown recycled paper (RP), black polyethylene film (PF) and soil without cover with weed removal (WR) and without weed removal (WW). RP and PF were efficient to control weeds. The soil temperature with RP was 8.2 and 2.1ºC lower than with PF and WR, respectively. The lettuce yield with RP was 14.5 and 28.3% higher than WR, and with PF, respectively. The water volume applied with RP was 26.5% lower, and WUE was 55.6% higher compared to WR. Soil cover with recycled paper controlled weeds, reduced soil temperature and water consumption and increased yield and water use efficiency in lettuce crop.
DOI: 10.17632/3n83mdt4cd.1
title: Caring for frail older people living alone in Italy: future housing solutions and responsibilities of family and public services

description: Data come from the study "Inclusive ageing in place” (IN-AGE), funded by Fondazione Cariplo (Italy), Grant N. 2017-0941, and regard a purposive sample of 120 individuals aged 65 years and over (males and females). It was a cross-sectional qualitative survey which in 2019 was conducted by face-to-face interviews to frail older people with functional/physical limitations, but without cognitive impairment, and living at home, alone or with a private personal care assistant (PCA), in three Italian Regions (Lombardy in the North, Marche in the Centre, and Calabria in the South). Both peripheral/degraded areas of urban sites and fragile rural locations were included, with regard to social and material vulnerability aspects (e.g. high presence of older people, high level of unemployment, low level of education, poor provision of services). The dataset regards in particular preference of participants on possible future housing solutions, and their opinion/orientation on care responsibilities of family and public services, also in the light of available/used care arrangements. A semi-structured interview was administered by expert interviewers. The issues were explored mainly with ad hoc open questions for the survey, adapted from previous studies. The difficulties in carrying out the activities of daily life were detected by administering the ADLs and IADLs scales. In order to provide the quantitative analysis (by means of Microsoft Excel 2019), qualitative dimensions were codified, in terms of absence/presence/frequency of the investigated aspects (e.g. help from the family and public services; possible future housing solutions, i.e., home, home with PCA, nursing home, cohabitation/proximity with children; caring responsibility. i.e., family, family and public service, public service). Regarding the level of physical limitations, four grades were provided, corresponding in turn to four levels of functional frailty (mild, moderate, high, very high). Moreover, socio-demographic dimensions are included. The verbatim transcriptions of the qualitative interviews are not publicly available due to privacy issues. Results showed that majority of seniors would like ageing at home, at least with a PCA, whereas moving to a nursing home is mainly a last chance. Also, they consider firstly the family as responsible of taking care of them, at least with the support of public services. In addition, some territorial differences emerged. An integrated model of LTC provision for older people, with both formal and informal supports, could allow ageing in place, with interventions based also on needs and preferences of both seniors and respective families. The dataset is provided in open format (Microsoft Excel) and includes the following: a “numeric” dataset regarding the un-labelled dimensions used for statistics elaboration; a codebook with the complete variables list and variables labels used.
DOI: 10.5281/zenodo.6477288
title: PMD hypomethylation human (hg19) neural network scores

description: <strong>Global loss of DNA methylation in mammalian genomes occurs cumulatively as a mitotic process during aging and cancer, primarily in Partially Methylated Domains (PMDs). It has been shown that local sequence context (100bp) has a strong effect on the rate of demethylation of individual CpG dinucleotides within PMDs. Here, we train a deep learning model to characterize this sequence dependence further, finding that methylation loss can be predicted from a CpG’s 150bp sequence context alone with an AUC of 0.95. We use re-methylation rates of newly synthesized DNA to show that CpGs with fast-loss sequence context are inefficiently re-methylated. Interestingly, we find that the 10% of CpGs predicted to have the “slowest” rate of loss lose almost no DNA methylation in healthy cell types. These same slow-loss CpGs lose a significant amount of DNA methylation in cancer, suggesting that they could be responsible for deregulation of genes and transposable elements that are associated with DNA hypomethylation in cancer.</strong> <strong>This directory contains the Sep. 20, 2020 version of the human (hg19) CpG hypomethylation Neural network scores in one gzip-compressed tsv file per chromosome.</strong> <strong>The Sep. 2020 Neural network score provides a prediction of the probability of each sequence to be a fast hypomethylation CpG, which was produced by a neural network model that used two independent input training datasets.</strong> <strong>Files included in this directory:</strong> <strong> - chr*. tsv.gz: Neural network score of each CpG in each chromosome, using hg19 coordinates. chrX and chrY are omitted.</strong> <strong> <br> Each row is a CG which provides (1) chromosome, (2) the corresponding C coordinate on the forward (watson) strand of the reference genome in one-based coordinates, (3) Neural network score, (4) number of CpGs within the 150bp sequence centered on this CpG, including the center CpG, (5) CpG is within a CpG island (0, no; 1, yes), CpG is within ENCODE blacklist (0, no; 1, yes)</strong> <strong> Here the CpG islands are the union set of Irizarry (Irizarry et al. 2009, Nat Genet), Takai-Jones (Takai et al. 2002, PNAS), Gardner-Gardin CGIs (Gardner-Gardin et al. 1987, J Mol Biol.). The blacklist was downloaded from https://github.com/Boyle-Lab/Blacklist/tree/master/lists.</strong>
DOI: 10.6084/m9.figshare.19923106.v1
title: Fluoride bioavailability on demineralized enamel by commercial mouthrinses

description: Abstract The ability of mouthrinses, available in the international market, to form reaction products on demineralized enamel (bioavailability test) was evaluated in vitro. Nine mouthrinses purchased in Chile were evaluated; eight formulated with NaF (one containing 100 µg F/mL and seven containing 226) and one with Na2FPO3 (226 µg F/mL as ion FPO3 ). Demineralized enamel slabs (n=15 per mouthrinse) were sectioned; one half was subjected to the assigned mouthrinse treatment for 10 min and the other half was used to obtain baseline data. Loosely bound and firmly bound fluoride formed on enamel were determined with an ion-specific electrode and the values were expressed in µg F/cm2. The concentration of fluoride and the pH of the mouthrinses were previously determined. Concentrations of loosely bound and firmly bound fluoride formed on enamel were independently analyzed by ANOVA and Tukey’s test (α=5%). The loosely bound and firmly bound fluoride concentrations formed ranged from 3.2 to 36.2 and 0.4 to 1.7, respectively. Loosely bound fluoride formed on enamel was significantly more effective in discriminating the effect of different commercial mouthrinses than firmly bound fluoride. Mouthrinses with 226 ppm F as NaF and low pH presented significantly greater bioavailability of fluoride on enamel than those with higher pH or lower NaF concentration. The mouthrinse with Na2FPO3 showed low reactivity. Although further studies are necessary, the findings showed that commercial fluoride-containing mouthrinses have important variations in enamel fluoride bioavailability, which may result in differences on anticaries efficacy.
DOI: 10.6084/m9.figshare.19923106
title: Fluoride bioavailability on demineralized enamel by commercial mouthrinses

description: Abstract The ability of mouthrinses, available in the international market, to form reaction products on demineralized enamel (bioavailability test) was evaluated in vitro. Nine mouthrinses purchased in Chile were evaluated; eight formulated with NaF (one containing 100 µg F/mL and seven containing 226) and one with Na2FPO3 (226 µg F/mL as ion FPO3 ). Demineralized enamel slabs (n=15 per mouthrinse) were sectioned; one half was subjected to the assigned mouthrinse treatment for 10 min and the other half was used to obtain baseline data. Loosely bound and firmly bound fluoride formed on enamel were determined with an ion-specific electrode and the values were expressed in µg F/cm2. The concentration of fluoride and the pH of the mouthrinses were previously determined. Concentrations of loosely bound and firmly bound fluoride formed on enamel were independently analyzed by ANOVA and Tukey’s test (α=5%). The loosely bound and firmly bound fluoride concentrations formed ranged from 3.2 to 36.2 and 0.4 to 1.7, respectively. Loosely bound fluoride formed on enamel was significantly more effective in discriminating the effect of different commercial mouthrinses than firmly bound fluoride. Mouthrinses with 226 ppm F as NaF and low pH presented significantly greater bioavailability of fluoride on enamel than those with higher pH or lower NaF concentration. The mouthrinse with Na2FPO3 showed low reactivity. Although further studies are necessary, the findings showed that commercial fluoride-containing mouthrinses have important variations in enamel fluoride bioavailability, which may result in differences on anticaries efficacy.
DOI: 10.6084/m9.figshare.19923107
title: Feldspathic and Lithium Disilicate Onlays with a 2-Year Follow-Up: Split-Mouth Randomized Clinical Trial

description: Abstract The present study was a prospective, controlled, randomized, clinical short-term trial aiming to evaluate the clinical performance of adhesively luted, lithium disilicate and feldspathic glass-ceramics onlays over a period up to 2 years. A total of 11 patients (7 female, 4 male; age range: 18-60 years, mean age: 39 years) were selected for this study. Each patient received a maximum of two restorations per group in a split-mouth-design. LD: Eleven onlays, performed with lithium disilicate-based ceramic (IPS e.max CAD, Ivoclar Vivadent, Schaan, Liechtenstein), and FP: Eleven onlays, performed with feldspathic ceramic (Vita Mark II, Vita Zanhfabrik, Bad Säckingen). Recalls were performed at 2 weeks (baseline = R1), 1 year (R2) and 2 years (R3) after the cementation by three calibrated blinded independent investigators using mirrors, magnifying eyeglasses, probes and bitewing radiographs. The postoperative sensitivity, secondary caries, marginal integrity, marginal discoloration, color match, surface roughness, tooth integrity, and restoration integrity were evaluated. The Friedman test was used to determine if there was a statistically significant difference in time-to-time comparison of the parameters in the ceramics restorations. A total of 95.4% of the restorations were clinically acceptable at the 2-year recall, without a difference for any evaluation parameter for both ceramic materials. Based on the 2-year data, the CAD-CAM onlays manufactured with feldspathic and lithium-disilicate based ceramics showed similar clinical performance.
DOI: 10.6084/m9.figshare.19923107.v1
title: Feldspathic and Lithium Disilicate Onlays with a 2-Year Follow-Up: Split-Mouth Randomized Clinical Trial

description: Abstract The present study was a prospective, controlled, randomized, clinical short-term trial aiming to evaluate the clinical performance of adhesively luted, lithium disilicate and feldspathic glass-ceramics onlays over a period up to 2 years. A total of 11 patients (7 female, 4 male; age range: 18-60 years, mean age: 39 years) were selected for this study. Each patient received a maximum of two restorations per group in a split-mouth-design. LD: Eleven onlays, performed with lithium disilicate-based ceramic (IPS e.max CAD, Ivoclar Vivadent, Schaan, Liechtenstein), and FP: Eleven onlays, performed with feldspathic ceramic (Vita Mark II, Vita Zanhfabrik, Bad Säckingen). Recalls were performed at 2 weeks (baseline = R1), 1 year (R2) and 2 years (R3) after the cementation by three calibrated blinded independent investigators using mirrors, magnifying eyeglasses, probes and bitewing radiographs. The postoperative sensitivity, secondary caries, marginal integrity, marginal discoloration, color match, surface roughness, tooth integrity, and restoration integrity were evaluated. The Friedman test was used to determine if there was a statistically significant difference in time-to-time comparison of the parameters in the ceramics restorations. A total of 95.4% of the restorations were clinically acceptable at the 2-year recall, without a difference for any evaluation parameter for both ceramic materials. Based on the 2-year data, the CAD-CAM onlays manufactured with feldspathic and lithium-disilicate based ceramics showed similar clinical performance.
DOI: 10.6084/m9.figshare.19923109.v1
title: Differential growth and yield responses of local potato cultivars from basic seed tubers

description: ABSTRACT Potato quality of seed tubers is a determinant factor that allows obtaining high yields, and its use must be a priority for a country or a producing region. Lack of information about how local cultivars perform differentially according to the environment has been identified in order to support potential seeds production programs. The research was carried out in 2018 at the Obonuco Research Center of Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), with the aim of analyzing the growth and yield of basic tuber seeds of five potato (Solanum tuberosum spp. andigena) cultivars. 1,080 seed tubers from each cultivar (treatments) were planted in a completely randomized block design and three replications. Mean comparison Tukey-Kramer (p≤0.05) test was performed for the yield analysis. From emergency to final harvest, destructive sampling was carried out every fifteen days in order to calculate dry matter and leaf area and establish growth models based on the accumulated degree-days (DD). The yield and proportion of seed tubers per plant were determined according to local resolution for seed certification in Colombia. Growth variables fitted better with the Hoerl, Gaussian, and third-degree polynomial models. The leaf area index of the potato cultivars reached the maximum value at 876 DD, with values ranging from 2.91 to 6.11 DD. The highest yield per plant was obtained by the Ica Única cultivar (2.73 kg plant-1). However, this cultivar showed the lowest percentage of seed tubers, with 50.59% compared to the others, which ranged between 80.82 and 87.40%. Differential potato growth responses through models based on the DD would explain the differences in final yield and seeds tuber production.
DOI: 10.6084/m9.figshare.19923110
title: Quality characterization, phenolic and carotenoid content of new orange, cream and yellow-fleshed sweetpotato genotypes

description: ABSTRACT Sweetpotato (Ipomoea batatas) is a root crop grown in many countries. This tuberous root is a source of energy, nutrients, and phytochemicals. In this study, bioactive compounds and physical and physicochemical qualities of sweetpotato genotypes were evaluated. Eight new genotypes of sweetpotato produced by Embrapa Hortaliças (orange-fleshed: MD09026-OF and MD09024-OF; cream-fleshed: MD09011-CF, MD09004-CF, MD10039-CF, and MD10004-CF; yellow-fleshed: MD09017-YF and MD12002-YF) and two cultivars used as controls (Beauregard and Brazlândia Roxa) were evaluated for color, soluble solids, dry matter, phenolic compounds, total carotenoids and β-carotene. Hue angles differed even between those sweetpotatoes with the same flesh color. The orange-fleshed genotypes MD09024-OF, MD09026-OF, and Beauregard, had the lowest L*, showing to be darker than the others. These sweetpotatoes also had the brightest flesh colors with higher C*. The orange-fleshed genotypes MD09026-OF and MD09024-OF were sweeter (10.55oBrix and 9.23oBrix) than Beauregard (5.12oBrix). Brazlândia Roxa had the highest dry matter content (38.05%), followed by the genotypes MD10004-CF, MD09017-YF, MD09026-OF MD10039-CF, and MD09011-CF, which showed similarity, ranging from 32.33% to 29.12%. The highest contents of total carotenoids were found for the orange-fleshed genotypes MD09026-OF (80.06 mg g-1) and MD09024-OF (70.56 mg g-1) and Beauregard (73.12 mg g-1). These same genotypes showed the highest total phenolic compounds (0.815 mg g-1 and 0.686 mg g-1, respectively). MD09026-OF showed the highest content of β-carotene (46.47 mg g-1). MD09026-OF was the most prominent genotype among those evaluated, as it showed the highest total carotenoid, β-carotene, phenolic compounds, and soluble solids content, in addition to a high dry matter content.
DOI: 10.6084/m9.figshare.19923109
title: Differential growth and yield responses of local potato cultivars from basic seed tubers

description: ABSTRACT Potato quality of seed tubers is a determinant factor that allows obtaining high yields, and its use must be a priority for a country or a producing region. Lack of information about how local cultivars perform differentially according to the environment has been identified in order to support potential seeds production programs. The research was carried out in 2018 at the Obonuco Research Center of Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), with the aim of analyzing the growth and yield of basic tuber seeds of five potato (Solanum tuberosum spp. andigena) cultivars. 1,080 seed tubers from each cultivar (treatments) were planted in a completely randomized block design and three replications. Mean comparison Tukey-Kramer (p≤0.05) test was performed for the yield analysis. From emergency to final harvest, destructive sampling was carried out every fifteen days in order to calculate dry matter and leaf area and establish growth models based on the accumulated degree-days (DD). The yield and proportion of seed tubers per plant were determined according to local resolution for seed certification in Colombia. Growth variables fitted better with the Hoerl, Gaussian, and third-degree polynomial models. The leaf area index of the potato cultivars reached the maximum value at 876 DD, with values ranging from 2.91 to 6.11 DD. The highest yield per plant was obtained by the Ica Única cultivar (2.73 kg plant-1). However, this cultivar showed the lowest percentage of seed tubers, with 50.59% compared to the others, which ranged between 80.82 and 87.40%. Differential potato growth responses through models based on the DD would explain the differences in final yield and seeds tuber production.