cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6083888
title: Electronic supplementary material tables and figures from Genome-wide single nucleotide polymorphism scan suggests adaptation to urbanization in an important pollinator, the red-tailed bumblebee (<i>Bombus lapidarius</i> L.)

description: Urbanization is considered a global threat to biodiversity; the growth of cities results in an increase in impervious surfaces, soil and air pollution, fragmentation of natural vegetation and invasion of non-native species along with numerous environmental changes, including the heat island phenomenon. The combination of these effects constitutes a challenge for both the survival and persistence of many native species, while also imposing altered selective regimes. Here, using 110 314 single nucleotide polymorphisms generated by restriction site-associated DNA sequencing, we investigated the genome-wide effects of urbanization on putative neutral and adaptive genomic diversity in a major insect pollinator, <i>Bombus lapidarius</i>, collected from nine German cities and nine paired rural sites. Overall, genetic differentiation among sites was low and there was no obvious genome-wide genetic structuring, suggesting the absence of strong effects of urbanization on gene flow. We, nevertheless, identified several loci under directional selection, a subset of which was associated with urban land use, including the percentage of impervious surface, surrounding each sampling site. Overall, our results provide evidence of local adaptation to urbanization in the face of gene flow in a highly mobile insect pollinator.
DOI: 10.6084/m9.figshare.6083837
title: Patterns of Reproductive and Seed Dispersal and Ecological Significance of the Clonal Spring Ephemeroid Plant Carex physodes in the Gurbantuggut Desert

description: ABSTRACT: Carex physodes is an ephemeral species in the cold desert of Gurbantunggut in Northwest China. It has both asexual and sexual reproductive patterns. The primary aims of this study were to characterize the reproduction systems and identify the role of fruit dispersal in the sexual reproduction of C. physodes. Aboveground and underground biomass, root-shoot ratio, inflorescence biomass, fruit-set of C. physodes were measured and dispersal of perigynia and achenes in the natural habitat and indoor condition were studied. The underground biomass of C. physodes was approximately 10 times more than the aboveground biomass. The most parts of aboveground biomass is allocated to the inflorescence, which suggests that C. physodes allocates most biomass to the reproductive part. C. physodes produces perigynium with a pericarp containing one achene. The perigynia disperse at a much greater distance than achenes at both 1 and 4 m s-1 wind velocity, and the floating time of perigynia in water was much longer than that of achenes. Perigynia can hold more water and adher soil much more easily than achenes, which suggests that perigynia are suitable for wind dispersal, and they also adapt to spread at a long distance by occasionally rainfall. However, achenes may remain near the mother plants and only disperse at short distances. C. physodes is morphologically and physiologically adapted to the cold desert environment via a combination of characters associated with the rhizomatous and perigynium. This adaption may increase the opportunity of survival and expansion of population of C. physodes.
DOI: 10.6084/m9.figshare.6083837.v1
title: Patterns of Reproductive and Seed Dispersal and Ecological Significance of the Clonal Spring Ephemeroid Plant Carex physodes in the Gurbantuggut Desert

description: ABSTRACT: Carex physodes is an ephemeral species in the cold desert of Gurbantunggut in Northwest China. It has both asexual and sexual reproductive patterns. The primary aims of this study were to characterize the reproduction systems and identify the role of fruit dispersal in the sexual reproduction of C. physodes. Aboveground and underground biomass, root-shoot ratio, inflorescence biomass, fruit-set of C. physodes were measured and dispersal of perigynia and achenes in the natural habitat and indoor condition were studied. The underground biomass of C. physodes was approximately 10 times more than the aboveground biomass. The most parts of aboveground biomass is allocated to the inflorescence, which suggests that C. physodes allocates most biomass to the reproductive part. C. physodes produces perigynium with a pericarp containing one achene. The perigynia disperse at a much greater distance than achenes at both 1 and 4 m s-1 wind velocity, and the floating time of perigynia in water was much longer than that of achenes. Perigynia can hold more water and adher soil much more easily than achenes, which suggests that perigynia are suitable for wind dispersal, and they also adapt to spread at a long distance by occasionally rainfall. However, achenes may remain near the mother plants and only disperse at short distances. C. physodes is morphologically and physiologically adapted to the cold desert environment via a combination of characters associated with the rhizomatous and perigynium. This adaption may increase the opportunity of survival and expansion of population of C. physodes.
DOI: 10.6084/m9.figshare.6083834
title: Transcatheter Aortic Valve Replacement: The Experience of One Brazilian Health Care Center

description: Abstract Objective: Transcatheter aortic valve replacement has been an alternative to invasive treatment for symptomatic severe aortic stenosis in high risk patients. The primary endpoint was 30-day and 1-year mortality from any cause. Secondary endpoints were to compare the clinical and echocardiographic variation pre-and post- transcatheter aortic valve replacement, and the occurrence of complications throughout a 4-year follow-up period. Methods: This prospective cohort, nestled to a multicenter study (Registro Brasileiro de Implante de Bioprótese por Cateter), describes the experience of a public tertiary center in transcatheter aortic valve replacement. All patients who underwent this procedure between October 2011 and February 2016 were included. Results: Fifty-eight patients underwent transcatheter aortic valve replacement. The 30-day all-cause mortality was 5.2% (n=3) and after 1 year was 17.2% (n=10). A significant improvement in New York Heart Association functional classification was observed when comparing pre-and post- transcatheter aortic valve replacement (III or IV 84.4% versus 5.8%; P&lt;0.001). A decline in peak was observed (P&lt;0.001) and mean (P&lt;0.001) systolic transaortic gradient. The results of peak and mean post-implant transaortic gradient were sustained after one year (P=0.29 and P=0.36, respectively). Left ventricular ejection fraction did not change significantly during follow-up (P=0.41). The most frequent complications were bleeding (28.9%), the need for permanent pacemaker (27.6%) and acute renal injury (20.6%). Conclusion: Mortality and complications in this study were consistent with worldwide experience. Transcatheter aortic valve replacement had positive clinical and hemodynamic results, when comparing pre-and post-procedure, and the hemodynamic profile of the prosthesis was sustained throughout follow-up.
DOI: 10.6084/m9.figshare.6083834.v1
title: Transcatheter Aortic Valve Replacement: The Experience of One Brazilian Health Care Center

description: Abstract Objective: Transcatheter aortic valve replacement has been an alternative to invasive treatment for symptomatic severe aortic stenosis in high risk patients. The primary endpoint was 30-day and 1-year mortality from any cause. Secondary endpoints were to compare the clinical and echocardiographic variation pre-and post- transcatheter aortic valve replacement, and the occurrence of complications throughout a 4-year follow-up period. Methods: This prospective cohort, nestled to a multicenter study (Registro Brasileiro de Implante de Bioprótese por Cateter), describes the experience of a public tertiary center in transcatheter aortic valve replacement. All patients who underwent this procedure between October 2011 and February 2016 were included. Results: Fifty-eight patients underwent transcatheter aortic valve replacement. The 30-day all-cause mortality was 5.2% (n=3) and after 1 year was 17.2% (n=10). A significant improvement in New York Heart Association functional classification was observed when comparing pre-and post- transcatheter aortic valve replacement (III or IV 84.4% versus 5.8%; P&lt;0.001). A decline in peak was observed (P&lt;0.001) and mean (P&lt;0.001) systolic transaortic gradient. The results of peak and mean post-implant transaortic gradient were sustained after one year (P=0.29 and P=0.36, respectively). Left ventricular ejection fraction did not change significantly during follow-up (P=0.41). The most frequent complications were bleeding (28.9%), the need for permanent pacemaker (27.6%) and acute renal injury (20.6%). Conclusion: Mortality and complications in this study were consistent with worldwide experience. Transcatheter aortic valve replacement had positive clinical and hemodynamic results, when comparing pre-and post-procedure, and the hemodynamic profile of the prosthesis was sustained throughout follow-up.
DOI: 10.6084/m9.figshare.6083831
title: Oxidative stability of crude palm oil after deep frying akara (Fried Bean Paste)

description: Abstract For this study 47 samples of crude palm oil (CPO) were collected, consisted of what had been used for 4 hours around the city of Salvador-Bahia. To evaluate the quality of CPO were analyzed: Total Polar Compound (TPC; %) by Adsorption Chromatography (AC), Testo 270 and Fricheck; Free fatty acids (FFA; % oleic acid), Color (CIELab); Total Carotenoids (TC; ppm), induction time (IT, h) and fatty acids composition (g 100 g-1). The TPC analysis by Testo 270 and Fricheck showed a poor linear correlation with AC (0.69 and 0.60 respectively). Otherwise 44.70% and 36.95% of samples were completely oxidized, according to CA and IT methods. All samples presented TC (11.01-331.45 ppm) and yellow color predominance. Trans fatty acids, C18:1t in 17.02% and C18:3t in 65.65% of the samples were identified. The results demonstrate the need in the deep frying control, respect to techniques used during process. As well the improvement of the quality of palm oil fresh, can minimize the degradation process during th deep frying.
DOI: 10.6084/m9.figshare.6083831.v1
title: Oxidative stability of crude palm oil after deep frying akara (Fried Bean Paste)

description: Abstract For this study 47 samples of crude palm oil (CPO) were collected, consisted of what had been used for 4 hours around the city of Salvador-Bahia. To evaluate the quality of CPO were analyzed: Total Polar Compound (TPC; %) by Adsorption Chromatography (AC), Testo 270 and Fricheck; Free fatty acids (FFA; % oleic acid), Color (CIELab); Total Carotenoids (TC; ppm), induction time (IT, h) and fatty acids composition (g 100 g-1). The TPC analysis by Testo 270 and Fricheck showed a poor linear correlation with AC (0.69 and 0.60 respectively). Otherwise 44.70% and 36.95% of samples were completely oxidized, according to CA and IT methods. All samples presented TC (11.01-331.45 ppm) and yellow color predominance. Trans fatty acids, C18:1t in 17.02% and C18:3t in 65.65% of the samples were identified. The results demonstrate the need in the deep frying control, respect to techniques used during process. As well the improvement of the quality of palm oil fresh, can minimize the degradation process during th deep frying.
DOI: 10.6084/m9.figshare.6083828
title: Attenuation of sound radiation in concrete structure through the reduction of mechanical vibration

description: Abstract The efficiency of sound irradiance in structure has direct relation with its vibratory movement. Dynamic vibration absorbers (DVAs) are a low cost viable option for reducing vibrations in passive structures. Secondary systems attached to the primary system (structure) in order to reduce vibration. In this work it was used an experimental modal analysis procedure (EMA) for vibratory responses through impulsive excitations to determine the natural frequencies and the location of points suitable for attachment of DVAs in a concrete beam. Later it was designed and built DVAs to reduce vibration in a frequency band where the response of the human auditory system is more sensitive. The best project configuration for DVAs was evaluated for sensitivity thereof with respect to the change of the loss factor of the viscoelastic material used. Obtained reduction of more than 36% over the considered frequency band and over 70% in the region of the resonance frequency in which the DVAs were tuned.
DOI: 10.6084/m9.figshare.6083828.v1
title: Attenuation of sound radiation in concrete structure through the reduction of mechanical vibration

description: Abstract The efficiency of sound irradiance in structure has direct relation with its vibratory movement. Dynamic vibration absorbers (DVAs) are a low cost viable option for reducing vibrations in passive structures. Secondary systems attached to the primary system (structure) in order to reduce vibration. In this work it was used an experimental modal analysis procedure (EMA) for vibratory responses through impulsive excitations to determine the natural frequencies and the location of points suitable for attachment of DVAs in a concrete beam. Later it was designed and built DVAs to reduce vibration in a frequency band where the response of the human auditory system is more sensitive. The best project configuration for DVAs was evaluated for sensitivity thereof with respect to the change of the loss factor of the viscoelastic material used. Obtained reduction of more than 36% over the considered frequency band and over 70% in the region of the resonance frequency in which the DVAs were tuned.
DOI: 10.6084/m9.figshare.6083825
title: The Role of Plant Growth Regulators in the Development of in vitro Flowering, Histology and Ultrastructural Studies in Impatiens balsamina cv. Dwarf Bush

description: ABSTRACT: An efficient protocol for in vitro flowering was successfully established for Impatiens balsamina cv Dwarf Bush, an important medicinal plant, through tissue culture techniques. Shoot, stem and petiole explants obtained from 4 week-old aseptic seedlings cultured on MS medium supplemented with different concentrations of plant growth regulator (PGR) were used for in vitro flower induction. Gibberellic acid (GA3), benzylaminopurine (BAP) and kinetin (Kin) treatment singly applied in MS media (pH 5.8), could all stimulate flowering at 23-26 oC with photoperiod of 16 hours light and 8 hours dark. It was observed that shoot explants were more responsive than stem explants in floral formation. Regeneration was achieved via direct organogenesis. For shoot explants, the treatment that induced the highest rate of in vitro flowering (7.30 ± 0.16 flowers per plantlet) was 1.0 mg L-1 GA3. Ultrastructural and histological analysis of in vivo and in vitro flowers were done to discover any somaclonal variation. This research described a simple protocol for rapid in vitro flowering that will be very beneficial for further breeding, cytological and molecular biology research.
DOI: 10.6084/m9.figshare.6083825.v1
title: The Role of Plant Growth Regulators in the Development of in vitro Flowering, Histology and Ultrastructural Studies in Impatiens balsamina cv. Dwarf Bush

description: ABSTRACT: An efficient protocol for in vitro flowering was successfully established for Impatiens balsamina cv Dwarf Bush, an important medicinal plant, through tissue culture techniques. Shoot, stem and petiole explants obtained from 4 week-old aseptic seedlings cultured on MS medium supplemented with different concentrations of plant growth regulator (PGR) were used for in vitro flower induction. Gibberellic acid (GA3), benzylaminopurine (BAP) and kinetin (Kin) treatment singly applied in MS media (pH 5.8), could all stimulate flowering at 23-26 oC with photoperiod of 16 hours light and 8 hours dark. It was observed that shoot explants were more responsive than stem explants in floral formation. Regeneration was achieved via direct organogenesis. For shoot explants, the treatment that induced the highest rate of in vitro flowering (7.30 ± 0.16 flowers per plantlet) was 1.0 mg L-1 GA3. Ultrastructural and histological analysis of in vivo and in vitro flowers were done to discover any somaclonal variation. This research described a simple protocol for rapid in vitro flowering that will be very beneficial for further breeding, cytological and molecular biology research.
DOI: 10.6084/m9.figshare.6083822
title: Inhibitory effect of Dendrobium officinale polysaccharide on human gastric cancer cell xenografts in nude mice

description: Abstract This study investigated the inhibitory effect of Dendrobium officinale polysaccharide (DOPA) on human gastric cancer cell SGC-7901 xenografts in nude mice. The nude mice with SGC-7901 xenografts were randomly divided into model, 5-fluorouracil (5-Fu), low-dose DOPA, middle-dose DOPA and high-dose DOPA group. The later four groups were intragastrically administrated with 100, 200 and 400 mg·kg–1·day–1 DOPA, 400 mg·kg–1·day–1 5-Fu and normal saline, respectively. After treatment for 20 days, the tumor inhibition rate of in high-dose DOPA group was basically equivalent to 5-Fu group. Compared with 5-Fu, DOPA had no obvious toxic side effect on spleen or thymus indexes, routine blood indexes or liver and kidney functions of nude mice. Compared with model group, the serum tumor necrosis factor-α and interleukin-2 levels in middle- and high-dose DOPA group were significantly increased (P &lt; 0.05), Bax protein expression was significantly increased (P &lt; 0.05), and Bcl-2 protein expression was significantly decreased (P &lt; 0.05). DOPA can inhibit the growth of SGC-7901 cell xenografts in nude mice. The mechanism may be related to its increase of serum TNF-α and IL-2 levels, up-regulation of Bax protein expression and down-regulation of Bcl-2 protein expression.
DOI: 10.6084/m9.figshare.6083822.v1
title: Inhibitory effect of Dendrobium officinale polysaccharide on human gastric cancer cell xenografts in nude mice

description: Abstract This study investigated the inhibitory effect of Dendrobium officinale polysaccharide (DOPA) on human gastric cancer cell SGC-7901 xenografts in nude mice. The nude mice with SGC-7901 xenografts were randomly divided into model, 5-fluorouracil (5-Fu), low-dose DOPA, middle-dose DOPA and high-dose DOPA group. The later four groups were intragastrically administrated with 100, 200 and 400 mg·kg–1·day–1 DOPA, 400 mg·kg–1·day–1 5-Fu and normal saline, respectively. After treatment for 20 days, the tumor inhibition rate of in high-dose DOPA group was basically equivalent to 5-Fu group. Compared with 5-Fu, DOPA had no obvious toxic side effect on spleen or thymus indexes, routine blood indexes or liver and kidney functions of nude mice. Compared with model group, the serum tumor necrosis factor-α and interleukin-2 levels in middle- and high-dose DOPA group were significantly increased (P &lt; 0.05), Bax protein expression was significantly increased (P &lt; 0.05), and Bcl-2 protein expression was significantly decreased (P &lt; 0.05). DOPA can inhibit the growth of SGC-7901 cell xenografts in nude mice. The mechanism may be related to its increase of serum TNF-α and IL-2 levels, up-regulation of Bax protein expression and down-regulation of Bcl-2 protein expression.
DOI: 10.6084/m9.figshare.6083819
title: Does the Interference of GR® Volunteer Corn Alters Stress Metabolism on Soybean?

description: ABSTRACT: The cultivation of GR® corn prior to soybean favors the occurrence of GR® volunteer corn plants interfering in soybean crops. The interference of volunteer corn causes the soybean yield losses, and the magnitude of losses varies with the corn density. The soybean yield losses can be partially explained by the occurrence of oxidative stress, which occurs by the higher content of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2). The objective of this study was to quantify H2O2 content and the activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) on soybean as a function of interference of populations of GR® volunteer corn originated from individual plants and clumps (clumps are seven corn plants emerged at the same point) in different times, as well as to determine wheter this interference alters stress metabolism on soybean. Quantification was performed at 20, 35 and 46 days after emergence (DAE) of soybean. The mean volunteer corn populations were 0, 0.5, 1, 2, 4, 8, 10 and 12 plants or clumps m-2. The results show changes in H2O2 content and SOD, CAT and APX activity as a response to interference with volunteer corn populations and origins. The higher activity was observated for SOD. Soybean yield reduce with the increase of populations of volunteer corn originated from individual plants and clumps.
DOI: 10.6084/m9.figshare.6083819.v1
title: Does the Interference of GR® Volunteer Corn Alters Stress Metabolism on Soybean?

description: ABSTRACT: The cultivation of GR® corn prior to soybean favors the occurrence of GR® volunteer corn plants interfering in soybean crops. The interference of volunteer corn causes the soybean yield losses, and the magnitude of losses varies with the corn density. The soybean yield losses can be partially explained by the occurrence of oxidative stress, which occurs by the higher content of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2). The objective of this study was to quantify H2O2 content and the activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) on soybean as a function of interference of populations of GR® volunteer corn originated from individual plants and clumps (clumps are seven corn plants emerged at the same point) in different times, as well as to determine wheter this interference alters stress metabolism on soybean. Quantification was performed at 20, 35 and 46 days after emergence (DAE) of soybean. The mean volunteer corn populations were 0, 0.5, 1, 2, 4, 8, 10 and 12 plants or clumps m-2. The results show changes in H2O2 content and SOD, CAT and APX activity as a response to interference with volunteer corn populations and origins. The higher activity was observated for SOD. Soybean yield reduce with the increase of populations of volunteer corn originated from individual plants and clumps.
DOI: 10.6084/m9.figshare.6083816
title: Morphometry and Germination of Passovia pyrifolia, Struthanthus marginatus and Phoradendron mucronatum Diaspores

description: ABSTRACT: The goal of this work was to characterize morphometrically diaspores from three species of mistletoes (Passovia pyrifolia, Struthanthus marginatus e Phoradendron mucronatum), as well as to study its germination behavior according to temperature and luminosity. In germination tests, the completely randomized design was used, in factor scheme 4 x 2, with 4 replications of 15 diaspores. The factors for the first test were different temperatures and two diaspore conditions (intact and without epicarp). In the second test, factors were different light qualities and two diaspore conditions. The analyzed variables were: germination percentage, germination rate index (GRI) and mean germination time (MGT). Mistletoe’s diaspores present an elliptic shape with viscin layer, an essential characteristic for the dispersion of the species. In addition to this, they present a distinction as for the moisture content when compared to the Loranthaceae and Viscaceae, where the latter presents the highest content. Germination is greater at the alternated temperature of 20-30 oC for P. pyrifolia and P. mucronatum diaspores and 20 oC for S. marginatus. Luminosity interfered in the germination of P. mucronatum and S. marginatus but did not influence P. pyrifolia. The diaspore condition influenced the germination of the three studied species, observing a significant increase in their germination when the epicarp was removed. Temperature and luminosity interfere directly in the GRI and MGT of the three mistletoes species.