DOI: 10.5061/dryad.7s7s7
title: Data from: Reed frog diversification in the Gulf of Guinea: overseas dispersal, the progression rule, and in situ speciation

description: Oceanic islands accumulate endemic species when new colonists diverge from
 source populations or by in situ diversification of resident island
 endemics. The relative importance of dispersal versus in situ speciation
 in generating diversity on islands varies with a number of archipelago
 characteristics including island size, age, and remoteness. Here we
 characterize inter-island dispersal and in situ speciation in frogs
 endemic to the Gulf of Guinea islands. Using mitochondrial sequence and
 genome-wide SNP data we demonstrate that dispersal proceeded from the
 younger island (São Tomé) to the older island (Príncipe) indicating that
 for organisms that disperse overseas on rafts, dispersal between islands
 may be determined by ocean currents and not island age. We find that
 dispersal between the islands is not ongoing, resulting in genotypically
 distinct but phenotypically similar lineages on the two islands. Finally,
 we demonstrate that in situ diversification on São Tomé Island likely
 proceeded in allopatry due to the geographic separation of breeding sites,
 resulting in phenotypically distinct species. We find evidence of
 hybridization between the species where their ranges are sympatric and the
 hybrid zone coincides with a transition from agricultural land to primary
 forest, indicating that anthropogenic development may have facilitated
 secondary contact between previously allopatric species.
description: Structure Main Data
 FileThis text file contains
 SNP data at 3644 loci for 78 samples of Hyperolius molleri and H.
 thomensis from São Tomé and Príncipe. The dataset includes only the first
 SNP from loci that are present in at least 75% of individuals in each of
 the three lineages (H. molleri Príncipe, H. molleri São Tomé, H. thomensis
 São Tomé). The first row of the file is the locus name, which corresponds
 to the locus ID in the catalog of loci generated by STACKS. Missing data
 indicated by “0".Islands_3644SNPs.txtSNAPP Input FileThis xml file contains SNP data at 467 loci for 5 samples of Hyperolius molleri from São Tomé, 5 samples of H. molleri from Príncipe, 5 H. thomensis from São Tomé, and 6 samples of the out group H. cinnamomeoventris from Gabon. The dataset includes biallelic SNPs present in at least two individuals in each of the four lineages. Homozygous positions indicated by 0 or 2, heterozygous positions indicated by 1, missing data indicated by “-“.IslandsOutGroup_467SNPs.xmlNewHybrids Input FileThis text file contains SNP data for 61 samples of Hyperolius molleri and H. thomensis from São Tomé. The complete set of 3644 SNPs used in the STRUCTURE analysis was pruned to only include SNPs with a minor allele frequency &gt; 0.2 (386 SNPs). The fifth row of the file is the locus name, which corresponds to the locus ID in the catalog of loci generated by STACKS. Samples with assignment probabilities &gt; 0.99 to either the H. molleri or H. thomensis deme in the STRUCTURE analysis are designated as “parental” genotypes with either the z0 or z1 flags. Missing data indicated by “00".SaoTomeHybrids_386SNPs.txt