cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.46c7wv
title: Occurrence Download

description: A dataset containing 154591 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ciconia ciconia",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Geometry POLYGON ((-6.6 29.26705, -6.6 50.94, 40.81373 50.94, 40.81373 29.26705, -6.6 29.26705))",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Observation, Material sample)"
 ]
}

 The dataset includes 154591 records from 34 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005780-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/h98pp-6mt63
title: MG1655 Sacculus

description: E. coli MG1655 sacculus .
description: <strong>Tilt Series Date:</strong> 2007-08-11</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655 sacculi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown to exponential phase in LB; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 4/2, BSA treated 5nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-08-11-21/rawdata/</p>MG1655_25.mrc,  Tilt Series (Pixel Size 0.78 nm), 394.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-08-11-21/rawdata/MG1655_25.mrc"
        > <i class="download icon"></i> Download </a></p>

        MG1655_25.rec,  Reconstruction (Pixel Size 0.78 nm), 379.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-08-11-21/3dimage_3214/MG1655_25.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jjwsj-vjr22
title: XL-10 Sacculus

description: E. coli XL-10 Gold sacculus .
description: <strong>Tilt Series Date:</strong> 2007-06-27</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> XL10-Gold</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown to exponential phase in LB; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 4/2, BSA treated 5nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-06-27-1/rawdata/</p>EC_04.mrc,  Tilt Series (Pixel Size 0.78 nm), 587.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-06-27-1/rawdata/EC_04.mrc"
        > <i class="download icon"></i> Download </a></p>

        EC_04.rec,  Reconstruction (Pixel Size 0.78 nm), 242.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-06-27-1/3dimage_3024/EC_04.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16990182
title: Mesoscale precipitation tracking to detect tropical cyclone extreme rainfall

description: This is an application of Large-Scale Precipitation Tracking (LPT) to the mesoscale. The averating period is reduced to 3 h, and the spatial filter width is reduced to 0.2 deg. (two IMERG grid points). It is aimed at tracking tropical cyclone (TC) rainfall prior to, during, and after the best track period. Specifically, it can detect post-TC TC remnant and extratropical transition (ET) rainfall after the storm is no longer tracked in best track. So far it has been applied to IMERG data.
DOI: 10.5281/zenodo.16990183
title: Mesoscale precipitation tracking to detect tropical cyclone extreme rainfall

description: This is an application of Large-Scale Precipitation Tracking (LPT) to the mesoscale. The averating period is reduced to 3 h, and the spatial filter width is reduced to 0.2 deg. (two IMERG grid points). It is aimed at tracking tropical cyclone (TC) rainfall prior to, during, and after the best track period. Specifically, it can detect post-TC TC remnant and extratropical transition (ET) rainfall after the storm is no longer tracked in best track. So far it has been applied to IMERG data.
DOI: 10.22002/gyrsr-9p217
title: Caulobacter, McpA fixed

description: correlated LM/EM .
description: <strong>Tilt Series Date:</strong> 2007-11-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, Photoshop</p>
<strong>Collaborators and Roles:</strong> Zemer Gitai</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells fixed after induction of McpA-mcherry fusion</p>
<strong>Sample Preparation:</strong> H2 London finder grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2007-11-07-4/rawdata/</p>MCPAcorrfix_box257Y_2.st,  Tilt Series (Pixel Size 1.25 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2007-11-07-4/rawdata/MCPAcorrfix_box257Y_2.st"
        > <i class="download icon"></i> Download </a></p>

        MCPAcorrfix_box257Y_2_full.rec,  Reconstruction (Pixel Size 2.5 nm), 604.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2007-11-07-4/3dimage_1134/MCPAcorrfix_box257Y_2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eyz72-7f616
title: Pva 111908 921

description: Prosthecobacter vanneervenii, 08dec03a_pb_005,
description: <strong>Tilt Series Date:</strong> 2008-12-03</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Prosthecobacter vanneervenii</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
  mpa2008-12-03-5_slicer10395.jpg: Prosthecobacter vanneervenii .  flat vesicle that resembles membrane mpa2008-12-03-5_slicer10398.jpg: Prosthecobacter vanneervenii .  pili
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2008-12-03-5/rawdata/</p>08dec03a_pb_005.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2008-12-03-5/rawdata/08dec03a_pb_005.mrc"
        > <i class="download icon"></i> Download </a></p>

        08dec03a_pb_005_full.rec,  Reconstruction (Pixel Size 1.9 nm), 1.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2008-12-03-5/3dimage_13222/08dec03a_pb_005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16990343
title: Pierce et al. (2025). Magnetostratigraphic constraints on the late Ediacaran paleomagnetic enigma.

description: Paleomagnetic data for the study of Pierce et al. (2025) Magnetostratigraphic constraints on the late Ediacaran paleomagnetic enigma. Science Advances.
DOI: 10.5281/zenodo.16990344
title: Pierce et al. (2025). Magnetostratigraphic constraints on the late Ediacaran paleomagnetic enigma.

description: Paleomagnetic data for the study of Pierce et al. (2025) Magnetostratigraphic constraints on the late Ediacaran paleomagnetic enigma. Science Advances.
DOI: 10.22002/rb6w5-jrw22
title: Hyphomonas 1st session 22.5kx

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2008-07-28</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
  jsa2008-07-28-26_slicer9490.jpg: Hyphomonas neptunium  .  Nested vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2008-07-28-26/rawdata/</p>08jul28b_box64_stack49.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2008-07-28-26/rawdata/08jul28b_box64_stack49.mrc"
        > <i class="download icon"></i> Download </a></p>

        08jul28b_box64_stack49_1k.rec,  Reconstruction (Pixel Size 1.9 nm), 78.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2008-07-28-26/3dimage_5254/08jul28b_box64_stack49_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6dqz4-tfc04
title: Hyphomonas session2 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division, potential pili
description: <strong>Tilt Series Date:</strong> 2008-08-29</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
  jsa2008-08-29-37_slicer9500.jpg: Hyphomonas neptunium  .  REZ
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2008-08-29-37/rawdata/</p>08aug29b_a1_081.mrc,  Tilt Series (Pixel Size 0.632 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2008-08-29-37/rawdata/08aug29b_a1_081.mrc"
        > <i class="download icon"></i> Download </a></p>

        08aug29b_a1_081_1k.rec,  Reconstruction (Pixel Size 1.264 nm), 91.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2008-08-29-37/3dimage_5390/08aug29b_a1_081_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g20gh-kp912
title: O. tauri @ G1

description: O. tauri cell synchronization .
description: <strong>Tilt Series Date:</strong> 2007-09-27</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Treated with HU for 12h, then PP for 3h, then fresh MK</p>
<strong>Sample Preparation:</strong> Q-foil R3.5/1, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-09-27-28/rawdata/</p>RCC745_HUPP_3h_8AM_01.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-09-27-28/rawdata/RCC745_HUPP_3h_8AM_01.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_HUPP_3h_8AM_01.rec,  Reconstruction (Pixel Size 2.52 nm), 308.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-09-27-28/3dimage_3274/RCC745_HUPP_3h_8AM_01.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mqxyx-6r964
title: Legionella_delta-lspHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-7/rawdata/</p>DG7_20190516_tomo0050.mrc,  Tilt Series (Pixel Size 0.26 nm), 882.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-7/rawdata/DG7_20190516_tomo0050.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG7_20190516_tomo0050_part31_11.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-7/3dimage_102615/DG7_20190516_tomo0050_part31_11.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/53xhe-fnn58
title: Legionella_delta-lspGHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS major and minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-18</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture, OD~4.8); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-18-11/rawdata/</p>DG8_201905180005.mrc,  Tilt Series (Pixel Size 0.26 nm), 882.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-18-11/rawdata/DG8_201905180005.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG8_201905180005_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-18-11/3dimage_102878/DG8_201905180005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/feqz0-jba23
title: Legionella_JV9246

description: Legionella_JV9246: DotF-sfGFP fusion to the wild-type gene on the chromosome (thyA) and ΔdotO.
description: <strong>Tilt Series Date:</strong> 2019-05-28</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-18/rawdata/</p>JV9246_area1_tomo0009.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-18/rawdata/JV9246_area1_tomo0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9246_area1_tomo0009_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-18/3dimage_103401/JV9246_area1_tomo0009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8vqr7-fp312
title: HeLa (Kyoto) - PM damaged

description: HeLa cell stably expresses CHMP4B-EGFP at low levels (courtesy MPI). grid - WY38_grid1
description: <strong>Tilt Series Date:</strong> 2018-04-01</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, caused plasma membrane damage using Laser and froze. Shrawan performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-04-01-1/rawdata/</p>TS3.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-04-01-1/rawdata/TS3.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS3_4bin_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-04-01-1/3dimage_102065/TS3_4bin_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>