DOI: 10.5061/dryad.ksn02v7b0
title: Data from: Heterogeneous patterns of heterozygosity loss in isolated populations of the threatened eastern barred bandicoot (Perameles gunnii)

description: Perameles gunnii is an endangered terrestrial marsupial on mainland
 Australia, but comparitively widespread in Tasmania. No research has yet
 aimed to assess population structure or genetic diversity in this state
 until now, where we assess genomic similarity and diversity using a ddRAD
 dataset of 117 Tasmanian animals. First, we assigned all samples to
 populations (or demes), according to their coordinate metadata and known
 geographic boundaries. We then test this hypothesis with
 population-ignorant genetic clustering analyses: PCA, Structure, and
 fineRADstructure. Validating seven (7) populations, four in a distinct
 "northern" group and three in a "southern" groupd, and
 also identify two populations displaying significant and unexpected
 isolation. We then looked to directly assess migration between these
 populations find little evidence, although genetic similarity within
 nothern and southern groups suggests that gradual mixing is ongoing within
 these areas. We assessed autosomal heterozygosity of individuals using a
 custom bioinformatic pipeline, and use a Kriging approach to plot regions
 of Tasmania where higher and lower heterozygosity individuals occur,
 finding unequal distribution of heterozygosity across geographic barriers
 that did not appear to affect genetic similarit-based analyses,
 potentially indicating emerging substructure within populations. We found
 individual heterozygosities varied within demes by up to a factor of two,
 and demes with low heterozygosity individuals also still contained those
 with high heterozygosity.  Our results provide novel insights
 into the relationship between isolation-driven genetic structure and local
 heterozygosity patterns. These may help improve translocation efforts, by
 identifying populations in need of assistance, and by providing an
 individualized metric for identifying source animals for translocation.
description: # *Perameles gunnnii* Tasmania 2008-2011
 Genotypic data and
 metadata for 117 Tasmanian Perameles gunnii, sampled from the wild between
 2008-2011, and 17 Victorian *Perameles gunnii* from Mt Rothwell
 Biodiversity Interpretation Centre, sampled from 2016. Samples provided as
 demultiplexed raw reads in .fq.gz format, and a draft scaffold genome is
 provided in .fa format. Metadata contains incomplete coordinate data
 approximated to two (2) decimal places to protect a sensitive species, as
 per The Global Biodiversity Information Facility (Category 3 sensitive
 species). Where exact coordinate data was unavailable, approximate data
 was inferred from the recorded locality, and whether coordinates are exact
 or approximate is indicated in the metadata. Metadata also contains
 incomplete sex data; where sex was unknown, NA has been used to indicate
 this. Additionally, publicly available, post-1990 sighing data for *P.
 gunnii* in Tasmania is available in ALA. Also, the distribution map figure
 that was generated using this data is available in the manuscript related
 to this repository. ## Description of the data and file structure
 Processing details for the raw reads and draft genome are in the
 associated manuscript. All programs used to process data, including
 version, are also indicated in the manuscript. All software used is open
 source. ## Code/Software This manuscript uses an autosomal heterozygosity
 pipeline available at