DOI: 10.22002/414jv-2s602
title: Agrobacterium tumefaciens A139

description: Bald strain with GFP tagged VirB8
description: <strong>Tilt Series Date:</strong> 2014-01-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown on plate, scraped off and grown o/n in a drop</p>
<strong>Sample Preparation:</strong> Vitrobot manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-4/rawdata/</p>A139_drop0004.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-4/rawdata/A139_drop0004.mrc"
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        A139_drop0004_full.rec,  Reconstruction (Pixel Size 0.0 nm), 623.6 MB  
        
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        slicer000.tif,  2.5 MB  
        
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        slicer001.tif,  2.5 MB  
        
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        slicer002.tif,  2.5 MB  
        
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        slicer003.tif,  2.5 MB  
        
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        slicer004.tif,  2.5 MB  
        
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        slicer005.tif,  2.5 MB  
        
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        slicer006.tif,  2.5 MB  
        
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