DOI: 10.5061/dryad.gf1vhhn0m
title: A bioprinted model of pregnant human uterine myometrium

description: We have utilized primary human uterine smooth muscle cells to bioprint
 myometrial tissue rings containing &gt;75% viable cells with
 elongated, smooth muscle morphology. Immunofluorescence confirmed
 expression of smooth muscle markers (caldesmon, alpha smooth muscle actin,
 and smooth muscle myosin), contractile-associated proteins (oxytocin
 receptor, prostaglandin receptors and connexin-43), and steroid hormone
 receptors (estrogen and progesterone receptors) characteristic of pregnant
 human uterine myometrium. Bioprinted tissue ring area contracted in
 response to physiological agonists oxytocin (p&lt;0.001),
 prostaglandin F2 alpha (p=0.003), and prostaglandin E2 (p&lt;0.001),
 and relaxed in response to the nitric oxide donor S-nitrosoglutathione
 (p=0.0004). 
description: # A bioprinted model of pregnant human uterine myometrium Dataset DOI:
 [10.5061/dryad.gf1vhhn0m](10.5061/dryad.gf1vhhn0m) ## Description of the
 data and file structure Total proteomic profiles (n=3 for each group) of
 parent myometrial tissue, primary uterine smooth muscle cells grown in
 2-dimensional culture, and synthetic myometrial tissues were collected and
 analyzed via TMT mass tag quantitative proteomics ### Files and variables
 #### File: TMT\_Proteomics\_Primary\_vs\_Parent\_Tissue.txt.xlsx
 **Description:** Tandem Mass Tag Quantitative Proteomic Analysis of
 Primary Cells vs Parent Tissue ##### Variables * **Column A (ID)– UNIPROT
 ID** * **Column B (Description)- Protein Description** * **Column C
 (CoveragePerc)- Percentage of protein coverage mapped** * **Column D
 (NumPep)- Number of Peptides Mapped** * **Column E (NumPSMSs)- Number of
 Peptide Scoring Matches Mapped** * **Column F (NumUniqPep)- Number of
 Unique Peptides Mapped** * **Column G (imput)- Where Imputed Peptides Used
 (Yes/NO)** * **Column H (numImpute)- Number of Imputed Peptides** *
 **Column I (protein)- UNIPROT ID** * **Column J (mean_P)- Mean Expression
 Level from TMT Analysis of Primary Cells** * **Column K (mean_T)- Mean
 Expression Level from TMT Analysis of Parent Tissue** * **Column L (pval)-
 P-value** * **Column M (adjpval)- Adjusted P-value** * **Column N (FC\_
 PvsT)- Fold Change Primary Cells vs Parent Tissue** #### File:
 TMT\_Proteomics\_Ring\_vs\_Parent\_Tissue.xlsx **Description:** Tandem
 Mass Tag Quantitative Proteomic Analysis of Ring vs Parent Tissue #####
 Variables * **Column A (ID)– UNIPROT ID** * **Column B (Description)-
 Protein Description** * **Column C (CoveragePerc)- Percentage of protein
 coverage mapped** * **Column D (NumPep)- Number of Peptides Mapped** *
 **Column E (NumPSMSs)- Number of Peptide Scoring Matches Mapped** *
 **Column F (NumUniqPep)- Number of Unique Peptides Mapped** * **Column G
 (imput)- Where Imputed Peptides Used (Yes/NO)** * **Column H (numImpute)-
 Number of Imputed Peptides** * **Column I (protein)- UNIPROT ID** *
 **Column J (mean_R)- Mean Expression Level from TMT Analysis of Ring** *
 **Column K (mean_T)- Mean Expression Level from TMT Analysis of Parent
 Tissue** * **Column L (pval)- P-value** * **Column M (adjpval)- Adjusted
 P-value** * **Column N (FC\_ RvsT)- Fold Change Ring vs Parent Tissue**
 #### File: TMT\_Proteomics\_Ring\_vs\_Primary.txt.xlsx
 **Description:** Tandem Mass Tag Quantitative Proteomic Analysis of Ring
 vs Primary Cells ##### Variables * **Column A (ID)– UNIPROT ID** *
 **Column B (Description)- Protein Description** * **Column C
 (CoveragePerc)- Percentage of protein coverage mapped** * **Column D
 (NumPep)- Number of Peptides Mapped** * **Column E (NumPSMSs)- Number of
 Peptide Scoring Matches Mapped** * **Column F (NumUniqPep)- Number of
 Unique Peptides Mapped** * **Column G (imput)- Where Imputed Peptides Used
 (Yes/NO)** * **Column H (numImpute)- Number of Imputed Peptides** *
 **Column I (protein)- UNIPROT ID** * **Column J (mean_R)- Mean Expression
 Level from TMT Analysis of Ring** * **Column K (mean_P)- Mean Expression
 Level from TMT Analysis of Primary Cells** * **Column L (pval)- P-value**
 * **Column M (adjpval)- Adjusted P-value** * **Column N (FC\_ RvsP)- Fold
 Change Ring vs Primary Cells** #### File: Live Dead 220516LB
 3DAnimation.avi **Description:** 3D cross section of human uterine smooth
 muscle rings stained to identify live cells (Calcein-AM Green) and dead
 cells (Propidium Iodide Red) with the nuclei labeled in blue (Hoechst).
 ##### Variables ## Code/software These are Excel Spreadsheets and .avi
 movie files that can be opened with any free software that supports this
 file type.