cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11839857.v1
title: Antibacterial and Antioxidant Activities of Slender Amaranth Weed

description: ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.
DOI: 10.6084/m9.figshare.11839839.v1
title: Application of Various Herbicides on Controlling Large and Narrow Leaf Weeds and Their Effects on Physiological and Agronomic Traits of Wheat

description: ABSTRACT: In order to examine application of various herbicides for controlling weeds in wheat crop, a field trial was conducted at the Agronomic Research Area, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa (KPK) during the year 2015-2016. The experiment was laid out in a randomized complete block design (RCBD) with split-plot arrangements having three replications. The main plot was comprised application time of herbicides while the use of herbicides was assigned to sub-plots. The data revealed that most of the weed parameters, physiological traits and agronomic attributes of crop plants were significantly affected by weed management practices including the use of herbicides at different time intervals over the weedy check. Hand weeding technique excelled all other weed management practices in almost all the parameters studied. This technique was found to be the most effective treatment against all prevailing weeds and obtained the highest grain yield of wheat under climatic conditions of Dera Ismail Khan.
DOI: 10.6084/m9.figshare.11839839
title: Application of Various Herbicides on Controlling Large and Narrow Leaf Weeds and Their Effects on Physiological and Agronomic Traits of Wheat

description: ABSTRACT: In order to examine application of various herbicides for controlling weeds in wheat crop, a field trial was conducted at the Agronomic Research Area, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa (KPK) during the year 2015-2016. The experiment was laid out in a randomized complete block design (RCBD) with split-plot arrangements having three replications. The main plot was comprised application time of herbicides while the use of herbicides was assigned to sub-plots. The data revealed that most of the weed parameters, physiological traits and agronomic attributes of crop plants were significantly affected by weed management practices including the use of herbicides at different time intervals over the weedy check. Hand weeding technique excelled all other weed management practices in almost all the parameters studied. This technique was found to be the most effective treatment against all prevailing weeds and obtained the highest grain yield of wheat under climatic conditions of Dera Ismail Khan.
DOI: 10.6084/m9.figshare.11839860
title: Bioactive Constituents of Shoot Extracts of Sisymbrium irio L. Against Fusarium oxysporum f. sp. cepae

description: ABSTRACT: The present study was carried out to check the antifungal potential of Sisymbrium irio L. shoot extract against Fusarium oxysporum f. sp. cepae (FOC). In preliminary bioassays, different concentrations (1 to 5%) of leaf, stem and fruit extracts were evaluated against FOC. All the extracts were effective against the pathogen. However, the leaf extract was found the most effective causing 25-41% decrease in FOC biomass. The fractionation of methanolic leaf extract was done by two organic solvents namely n-hexane and chloroform. Different concentrations (1.56 to 200 mg mL-1) of these fractions were tested against FOC. The n-hexane and chloroform fractions showed inhibitory activity against the pathogen and resulted in 77-93% and 80-96% reduction in biomass of FOC, respectively. GC-MS analysis showed the presence of 24 compounds in n-hexane and 4 compounds in chloroform fraction. In n-hexane fraction, β-sitosterol (18.64%) was the most abundant compound followed by orotic acid, bis(tert-butyldimethylsilyl)-, tert-butyldimethylsilyl ester (12.18%), 10-octadecenoic acid, methyl ester (7.90%) and 1,2-benzenedicarboxylic acid, diisooctyl ester (6.05%). Major compounds identified in chloroform fraction were 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (50.82%) and di-n-octyl phthalate (33.00%). This study concludes that n-hexane and chloroform fractions of methanolic leaf extract of S. irio contain potent antifungal constituents for the management of FOC.
DOI: 10.6084/m9.figshare.11839860.v1
title: Bioactive Constituents of Shoot Extracts of Sisymbrium irio L. Against Fusarium oxysporum f. sp. cepae

description: ABSTRACT: The present study was carried out to check the antifungal potential of Sisymbrium irio L. shoot extract against Fusarium oxysporum f. sp. cepae (FOC). In preliminary bioassays, different concentrations (1 to 5%) of leaf, stem and fruit extracts were evaluated against FOC. All the extracts were effective against the pathogen. However, the leaf extract was found the most effective causing 25-41% decrease in FOC biomass. The fractionation of methanolic leaf extract was done by two organic solvents namely n-hexane and chloroform. Different concentrations (1.56 to 200 mg mL-1) of these fractions were tested against FOC. The n-hexane and chloroform fractions showed inhibitory activity against the pathogen and resulted in 77-93% and 80-96% reduction in biomass of FOC, respectively. GC-MS analysis showed the presence of 24 compounds in n-hexane and 4 compounds in chloroform fraction. In n-hexane fraction, β-sitosterol (18.64%) was the most abundant compound followed by orotic acid, bis(tert-butyldimethylsilyl)-, tert-butyldimethylsilyl ester (12.18%), 10-octadecenoic acid, methyl ester (7.90%) and 1,2-benzenedicarboxylic acid, diisooctyl ester (6.05%). Major compounds identified in chloroform fraction were 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (50.82%) and di-n-octyl phthalate (33.00%). This study concludes that n-hexane and chloroform fractions of methanolic leaf extract of S. irio contain potent antifungal constituents for the management of FOC.
DOI: 10.6084/m9.figshare.11840010
title: Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

description: ABSTRACT: In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five groups, being one group formed just by the susceptible biotype and in the others grouped by biotypes from distinct locations in the same group The high genetic diversity of C. sumatrensis indicates that the biotypes may show different responses to different management strategies, and that the mechanisms of resistance to herbicides and characteristics of evolution of populations due to adaptability may be some of the factors involved in the genetic variability of the species.
DOI: 10.6084/m9.figshare.11840010.v1
title: Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

description: ABSTRACT: In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five groups, being one group formed just by the susceptible biotype and in the others grouped by biotypes from distinct locations in the same group The high genetic diversity of C. sumatrensis indicates that the biotypes may show different responses to different management strategies, and that the mechanisms of resistance to herbicides and characteristics of evolution of populations due to adaptability may be some of the factors involved in the genetic variability of the species.
DOI: 10.6084/m9.figshare.11839701
title: Understanding the Competitive Effects of Blessed Milkthistle Densities on Wheat

description: ABSTRACT: Weed-induced yield loss in wheat crop is a great threat to food security in Pakistan. A comprehensive understanding of weed-crop competition is very important to develop sustainable and cost-effective weed management. For this purpose, two-year field studies were conducted to determine the effect of different blessed milkthistle densities on the phenology and yield of wheat crop in a rice-wheat cropping scheme in Sargodha, Pakistan during 2013-2014 and 2014-2015. The experiment comprised seven treatments: control (weed free), weedy check (weedy without any control) and blessed milkthistle densities of 5, 10, 15, 20 and 25 plants m-2. In response to increasing weed density, a gradual reduction in yield and yield-related traits of wheat was noted. Compared to the weed-free control, a significant reduction in number of productive tillers m-2 (20% and 18%), plant height (15% and 18%), spike length (19% and 26%), number of grains spike-1 (23% and 26%), 1000 grain weight (28% and 28%), grain (29% and 30%) and biological (20% and 24%) yields of wheat occurred at and beyond blessed milkthistle density of 5 plants m-2 during 2013-2014 and 2014-2015 respectively. It can be concluded that blessed milkthistle weed must be controlled if its population density reaches 5 plants m-2 in order to avoid significant grain yield losses in wheat.
DOI: 10.6084/m9.figshare.11839701.v1
title: Understanding the Competitive Effects of Blessed Milkthistle Densities on Wheat

description: ABSTRACT: Weed-induced yield loss in wheat crop is a great threat to food security in Pakistan. A comprehensive understanding of weed-crop competition is very important to develop sustainable and cost-effective weed management. For this purpose, two-year field studies were conducted to determine the effect of different blessed milkthistle densities on the phenology and yield of wheat crop in a rice-wheat cropping scheme in Sargodha, Pakistan during 2013-2014 and 2014-2015. The experiment comprised seven treatments: control (weed free), weedy check (weedy without any control) and blessed milkthistle densities of 5, 10, 15, 20 and 25 plants m-2. In response to increasing weed density, a gradual reduction in yield and yield-related traits of wheat was noted. Compared to the weed-free control, a significant reduction in number of productive tillers m-2 (20% and 18%), plant height (15% and 18%), spike length (19% and 26%), number of grains spike-1 (23% and 26%), 1000 grain weight (28% and 28%), grain (29% and 30%) and biological (20% and 24%) yields of wheat occurred at and beyond blessed milkthistle density of 5 plants m-2 during 2013-2014 and 2014-2015 respectively. It can be concluded that blessed milkthistle weed must be controlled if its population density reaches 5 plants m-2 in order to avoid significant grain yield losses in wheat.
DOI: 10.6084/m9.figshare.19962517
title: Meloidogyne Species Associated with Weeds in Rio Grande do Sul

description: ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was detected in 15.6% of the samples. M. incognita Est. I2 (Rm: 1.0, 1.1), M. ethiopica Est. E3 (Rm: 0.9, 1.15, 1.30), M. enterolobii Est. M2 (Rm: 0.7, 0.75, 0.9, 0.95) and M. hapla Est. H1 (Rm: 1.17) in 13.3%, 8.9%, 6.7% and 2.2% of the samples, respectively. Therefore, knowledge of the range of host plants to different species of the root-knot nematode can positively contribute to the adoption of management practices that allow the reduction of their populations in the soil.
DOI: 10.6084/m9.figshare.19962149
title: Safe ultrasound-guided percutaneous tracheostomy in eight steps and necessary precautions in COVID-19 patients

description: ABSTRACT Percutaneous tracheostomy has been considered the standard method today, the bronchoscopy-guided technique being the most frequently performed. A safe alternative is ultrasound-guided percutaneous tracheostomy, which can be carried out by the surgeon, avoiding the logistical difficulties of having a specialist in bronchoscopy. Studies prove that the efficacy and safety of the ultrasound-guided technique are similar when compared to the bronchoscopy-guided one. Thus, it is of paramount importance that surgeons have ultrasound-guided percutaneous tracheostomy as a viable and beneficial alternative to the open procedure. In this article, we describe eight main steps in performing ultrasound-guided percutaneous tracheostomy, highlighting essential technical points that can reduce the risk of complications from the procedure. Furthermore, we detail some precautions that one must observe to reduce the risk of aerosolization and contamination of the team when percutaneous tracheostomy is indicated in patients with COVID-19.
DOI: 10.6084/m9.figshare.19962577
title: Ziyuglycoside II-induced apoptosis in human gastric carcinoma BGC-823 cells by regulating Bax/Bcl-2 expression and activating caspase-3 pathway

description: Ziyuglycoside II is an active compound of Sanguisorba officinalis L. that has anti-inflammation, antioxidation, antibiosis, and homeostasis properties. We report here on the anticancer effect of ziyuglycoside II on human gastric carcinoma BGC-823 cells. We investigated the effects of ziyuglycoside II on cell growth, cell cycle, and cell apoptosis of this cell line. Our results revealed that ziyuglycoside II could inhibit the proliferation of BGC-823 cells by inducing apoptosis but not cell cycle arrest, which was associated with regulation of Bax/Bcl-2 expression, and activation of the caspase-3 pathway. Our study is the first to report the antitumor potential of ziyuglycoside II in BGC-823 gastric cancer cells. Ziyuglycoside II may become a potential therapeutic agent against gastric cancer in the future.
DOI: 10.6084/m9.figshare.19962577.v1
title: Ziyuglycoside II-induced apoptosis in human gastric carcinoma BGC-823 cells by regulating Bax/Bcl-2 expression and activating caspase-3 pathway

description: Ziyuglycoside II is an active compound of Sanguisorba officinalis L. that has anti-inflammation, antioxidation, antibiosis, and homeostasis properties. We report here on the anticancer effect of ziyuglycoside II on human gastric carcinoma BGC-823 cells. We investigated the effects of ziyuglycoside II on cell growth, cell cycle, and cell apoptosis of this cell line. Our results revealed that ziyuglycoside II could inhibit the proliferation of BGC-823 cells by inducing apoptosis but not cell cycle arrest, which was associated with regulation of Bax/Bcl-2 expression, and activation of the caspase-3 pathway. Our study is the first to report the antitumor potential of ziyuglycoside II in BGC-823 gastric cancer cells. Ziyuglycoside II may become a potential therapeutic agent against gastric cancer in the future.
DOI: 10.6084/m9.figshare.5669857
title: Potential benefit of (-)-epigallocatechin-3-gallate for macrovascular complications in diabetes

description: Vascular problems are the most common complications in diabetes. Substantial evidence from epidemiological and pathophysiological studies show that hyperglycemia is a major risk factor for macrovascular complications in patients with diabetes. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, is known to exert a variety of cardiovascular beneficial effects. The protective effects of EGCG in diabetes are also evident. However, whether EGCG is beneficial against macrovascular complications that occur in diabetes remains unknown. Our previous studies demonstrated that treatment of EGCG inhibits high glucose-induced vascular smooth muscle cell proliferation and suppresses high glucose-mediated vascular inflammation in human umbilical vein endothelial cells. Therefore, we hypothesize that EGCG might be an effective potential candidate to reduce the macrovascular complications in diabetes.
DOI: 10.6084/m9.figshare.5669857.v1
title: Potential benefit of (-)-epigallocatechin-3-gallate for macrovascular complications in diabetes

description: Vascular problems are the most common complications in diabetes. Substantial evidence from epidemiological and pathophysiological studies show that hyperglycemia is a major risk factor for macrovascular complications in patients with diabetes. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, is known to exert a variety of cardiovascular beneficial effects. The protective effects of EGCG in diabetes are also evident. However, whether EGCG is beneficial against macrovascular complications that occur in diabetes remains unknown. Our previous studies demonstrated that treatment of EGCG inhibits high glucose-induced vascular smooth muscle cell proliferation and suppresses high glucose-mediated vascular inflammation in human umbilical vein endothelial cells. Therefore, we hypothesize that EGCG might be an effective potential candidate to reduce the macrovascular complications in diabetes.
DOI: 10.6084/m9.figshare.7899875
title: Physical exercise-induced fatigue: the role of serotonergic and dopaminergic systems

description: Brain serotonin and dopamine are neurotransmitters related to fatigue, a feeling that leads to reduced intensity or interruption of physical exercises, thereby regulating performance. The present review aims to present advances on the understanding of fatigue, which has recently been proposed as a defense mechanism instead of a “physiological failure” in the context of prolonged (aerobic) exercises. We also present recent advances on the association between serotonin, dopamine and fatigue. Experiments with rodents, which allow direct manipulation of brain serotonin and dopamine during exercise, clearly indicate that increased serotoninergic activity reduces performance, while increased dopaminergic activity is associated with increased performance. Nevertheless, experiments with humans, particularly those involving nutritional supplementation or pharmacological manipulations, have yielded conflicting results on the relationship between serotonin, dopamine and fatigue. The only clear and reproducible effect observed in humans is increased performance in hot environments after treatment with inhibitors of dopamine reuptake. Because the serotonergic and dopaminergic systems interact with each other, the serotonin-to-dopamine ratio seems to be more relevant for determining fatigue than analyzing or manipulating only one of the two transmitters. Finally, physical training protocols induce neuroplasticity, thus modulating the action of these neurotransmitters in order to improve physical performance.
DOI: 10.6084/m9.figshare.7899875.v1
title: Physical exercise-induced fatigue: the role of serotonergic and dopaminergic systems

description: Brain serotonin and dopamine are neurotransmitters related to fatigue, a feeling that leads to reduced intensity or interruption of physical exercises, thereby regulating performance. The present review aims to present advances on the understanding of fatigue, which has recently been proposed as a defense mechanism instead of a “physiological failure” in the context of prolonged (aerobic) exercises. We also present recent advances on the association between serotonin, dopamine and fatigue. Experiments with rodents, which allow direct manipulation of brain serotonin and dopamine during exercise, clearly indicate that increased serotoninergic activity reduces performance, while increased dopaminergic activity is associated with increased performance. Nevertheless, experiments with humans, particularly those involving nutritional supplementation or pharmacological manipulations, have yielded conflicting results on the relationship between serotonin, dopamine and fatigue. The only clear and reproducible effect observed in humans is increased performance in hot environments after treatment with inhibitors of dopamine reuptake. Because the serotonergic and dopaminergic systems interact with each other, the serotonin-to-dopamine ratio seems to be more relevant for determining fatigue than analyzing or manipulating only one of the two transmitters. Finally, physical training protocols induce neuroplasticity, thus modulating the action of these neurotransmitters in order to improve physical performance.