cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30067420.v3
title: <b>Role of the Chaperone ClpB in</b><b> </b><b>Mycoplasma bovis</b><b> Virulence: Implications for </b><b>stress response, a</b><b>dhesion, </b><b>b</b><b>iofilm </b><b>d</b><b>evelopment, and </b><b>secreted n</b><b>uclease </b><b>activity</b>

description: ClpB, an ATP-dependent molecular chaperone belonging to the Hsp100/Clp subfamily of AAA+ ATPases, plays a crucial role in protein disaggregation, thereby enhancing bacterial survival under stress conditions. Despite its well-conserved function in prokaryotes, the specific contributions of ClpB to the pathogenesis of the ruminant pathogen Mycoplasma bovis remain largely unexplored. In this study, we identified and functionally characterized a ClpB homolog in M. bovis. Biochemical assays confirmed that the recombinant ClpB protein exhibits intrinsic ATPase activity and, in cooperation with the DnaK chaperone system, efficiently mediates protein disaggregation in vitro. Through genome-wide transposon mutagenesis of the M. bovis HB0801 strain, we generated ClpB-deficient mutants that maintained normal growth kinetics and morphology at 37°C but exhibited significant growth defects under thermal (42°C) and osmotic stress conditions. Phenotypic characterization revealed that ClpB disruption attenuated key virulence attributes, including: impaired host cell adhesion capacity, significant reduction in biofilm formation (p&lt;0.01), diminished induction of proinflammatory cytokines (IL-1β, IL-6, TNF-α) in infected BoMac cells, decreased extracellular nuclease activity. Furthermore, the reduced virulence of the ClpB mutant was investigated by DIA proteomic analyses, which revealed that the ClpB mutant strain altered distinct protein expression patterns related to protein degration, including serine-type peptidase activity, serine hydrolase activity, and chaperone-mediated protein folding that contribute to the stress response and virulence. These findings collectively demonstrate that ClpB serves as a multifunctional virulence determinant in M. bovis, orchestrating stress adaptation, host-pathogen interactions, and pathogenic potential through modulation of both protein quality control systems and virulence-associated pathways.
DOI: 10.6084/m9.figshare.30067420.v2
title: <b>Role of the Chaperone ClpB in</b><b> </b><b>Mycoplasma bovis</b><b> Virulence: Implications for </b><b>stress response, a</b><b>dhesion, </b><b>b</b><b>iofilm </b><b>d</b><b>evelopment, and </b><b>secreted n</b><b>uclease </b><b>activity</b>

description: ClpB, an ATP-dependent molecular chaperone belonging to the Hsp100/Clp subfamily of AAA+ ATPases, plays a crucial role in protein disaggregation, thereby enhancing bacterial survival under stress conditions. Despite its well-conserved function in prokaryotes, the specific contributions of ClpB to the pathogenesis of the ruminant pathogen Mycoplasma bovis remain largely unexplored. In this study, we identified and functionally characterized a ClpB homolog in M. bovis. Biochemical assays confirmed that the recombinant ClpB protein exhibits intrinsic ATPase activity and, in cooperation with the DnaK chaperone system, efficiently mediates protein disaggregation in vitro. Through genome-wide transposon mutagenesis of the M. bovis HB0801 strain, we generated ClpB-deficient mutants that maintained normal growth kinetics and morphology at 37°C but exhibited significant growth defects under thermal (42°C) and osmotic stress conditions. Phenotypic characterization revealed that ClpB disruption attenuated key virulence attributes, including: impaired host cell adhesion capacity, significant reduction in biofilm formation (p&lt;0.01), diminished induction of proinflammatory cytokines (IL-1β, IL-6, TNF-α) in infected BoMac cells, decreased extracellular nuclease activity. Furthermore, the reduced virulence of the ClpB mutant was investigated by DIA proteomic analyses, which revealed that the ClpB mutant strain altered distinct protein expression patterns related to protein degration, including serine-type peptidase activity, serine hydrolase activity, and chaperone-mediated protein folding that contribute to the stress response and virulence. These findings collectively demonstrate that ClpB serves as a multifunctional virulence determinant in M. bovis, orchestrating stress adaptation, host-pathogen interactions, and pathogenic potential through modulation of both protein quality control systems and virulence-associated pathways.
DOI: 10.15468/dl.erjuqz
title: Occurrence Download

description: A dataset containing 325 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium acuminatum",
 "Country is Russian Federation",
 "HasCoordinate is true"
 ]
}

 The dataset includes 325 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024603-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.xwjcj6
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "TaxonKey is Eupsophus insularis"
 ]
}

 The dataset includes 6 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024604-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ye7nu3
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium bonus-henricus",
 "Country is Bulgaria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 13 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024605-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.975u7y
title: Occurrence Download

description: A dataset containing 20 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium bonus-henricus",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 20 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024606-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.w8x6r8
title: Occurrence Download

description: A dataset containing 34303 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Elagatis bipinnulata"
 ]
}

 The dataset includes 34303 records from 240 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024607-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c3x58w
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium multifidum",
 "Country is Bulgaria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024608-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wf3brp
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium murale",
 "Country is Bulgaria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024610-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.kmmj9y
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium murale",
 "Country is Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024612-250920141307145/datasets/export for details.
DOI: 10.15468/dl.955fjq
title: Occurrence Download

description: A dataset containing 19 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium murale",
 "Country is Egypt",
 "HasCoordinate is true"
 ]
}

 The dataset includes 19 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024611-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fakua5
title: Occurrence Download

description: A dataset containing 14854117 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Continent is one of (Africa, Asia, Oceania, South America)",
 "Geometry POLYGON((-180 -58,180 -58,180 84,-180 84,-180 -58))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "License is one of (CC-BY 4.0, CC-BY-NC 4.0)",
 "OccurrenceStatus is Present",
 "TaxonKey is Animalia",
 "Year is 2016"
 ]
}

 The dataset includes 14854117 records from 652 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024560-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nypg3r
title: Occurrence Download

description: A dataset containing 44 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium murale",
 "Country is South Africa",
 "HasCoordinate is true"
 ]
}

 The dataset includes 44 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024614-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.w2uvvs
title: Occurrence Download

description: A dataset containing 12 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium murale",
 "Country is Yemen",
 "HasCoordinate is true"
 ]
}

 The dataset includes 12 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024615-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dqm584
title: Occurrence Download

description: A dataset containing 30781 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Pseudocaranx dentex"
 ]
}

 The dataset includes 30781 records from 171 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024616-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.n8svee
title: Occurrence Download

description: A dataset containing 1334 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-70.25171 -15.93754,-69.65942 -15.93754,-69.65942 -15.57731,-70.25171 -15.57731,-70.25171 -15.93754))",
 "TaxonKey is Gallinula chloropus"
 ]
}

 The dataset includes 1334 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024617-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qyyym6
title: Occurrence Download

description: A dataset containing 1086 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-70.26472 -15.98417,-69.65385 -15.98417,-69.65385 -15.35604,-70.26472 -15.35604,-70.26472 -15.98417))",
 "OccurrenceStatus is Present",
 "TaxonKey is Rollandia microptera"
 ]
}

 The dataset includes 1086 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024618-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.my6j5c
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium polyspermum",
 "Country is Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024620-250920141307145/datasets/export for details.
DOI: 10.15468/dl.ax7mwf
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium polyspermum",
 "Country is Bulgaria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024619-250920141307145/datasets/export for details.
DOI: 10.15468/dl.5dj5n2
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium polyspermum",
 "Country is South Africa",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024621-250920141307145/datasets/export for details.
DOI: 10.15468/dl.caakcv
title: Occurrence Download

description: A dataset containing 43 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chenopodium polyspermum",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 43 records from 20 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024622-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ztq4t2
title: Occurrence Download

description: A dataset listing the 2 species recorded in GBIF matching the query:
{
 "DatasetKey" : [
 "is Revisiting and rediscovering the tarantulas (Araneae, Theraphosidae) of Culapnitan (Libmanan) Caves in the Philippines: troglomorphism, taxonomy, phylogeny and ecological niche"
 ]
}

 The dataset's 2 records were derived from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024613-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.mkdwun
title: Occurrence Download

description: A dataset containing 23785 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Seriola dumerili"
 ]
}

 The dataset includes 23785 records from 220 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024623-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ux8th7
title: Occurrence Download

description: A dataset containing 22225510 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Continent is one of (Africa, Asia, Oceania, Europe, South America)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "License is one of (CC-BY-NC 4.0, CC-BY 4.0)",
 "Month is one of (July, August, September, October, November, December)",
 "OccurrenceStatus is Present",
 "TaxonKey is Animalia",
 "Year is 2017"
 ]
}

 The dataset includes 22225510 records from 3344 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024563-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.26232722
title: Isotope hydrology of the intermontane Elk Valley, British Columbia: an assessment of water resources around coal mining operations

description: This study aimed to synthesise and interpret stable isotopic data (<i>δ</i><sup>2</sup>H and <i>δ</i><sup>18</sup>O) from various sources to understand the isotope hydrology around coal mine operations in Elk Valley, B.C., Canada. The data, including precipitation, groundwaters, seeps, and mine rock drains, were used to construct a local meteoric water line (LMWL) for the Elk Valley, evaluate the spatiotemporal isotopic composition of its groundwater, and assess mine seepage and mine rock drain discharge. The study revealed a robust LMWL relation (<i>δ</i><sup>2</sup>H = 7.4 ± 0.2 · <i>δ</i><sup>18</sup>O – 4.3 ± 4.1). The groundwater and seep data indicated a winter season bias and a north–south latitudinal gradient, suggesting rapid near-surface groundwater flow without significant post-precipitation evaporation. Porewater isotope samples from unsaturated mine rock piles (MRPs) showed site-specific evaporation patterns, potentially due to convective air flows or exothermic sulphide oxidation. This research revealed the influence of groundwater and meltwater on rock drain discharge. Based on evaporative mass balance calculations, MRPs seasonally contributed ca. 5 %(December base flow) and 22 % (snowmelt) to drain discharge. The findings underscore the value of stable isotope data collections in the Elk Valley to help better define and quantify the hydrology–hydrogeology, including a better understanding of evaporative conditions in MRPs.
DOI: 10.6084/m9.figshare.26232722.v1
title: Isotope hydrology of the intermontane Elk Valley, British Columbia: an assessment of water resources around coal mining operations

description: This study aimed to synthesise and interpret stable isotopic data (<i>δ</i><sup>2</sup>H and <i>δ</i><sup>18</sup>O) from various sources to understand the isotope hydrology around coal mine operations in Elk Valley, B.C., Canada. The data, including precipitation, groundwaters, seeps, and mine rock drains, were used to construct a local meteoric water line (LMWL) for the Elk Valley, evaluate the spatiotemporal isotopic composition of its groundwater, and assess mine seepage and mine rock drain discharge. The study revealed a robust LMWL relation (<i>δ</i><sup>2</sup>H = 7.4 ± 0.2 · <i>δ</i><sup>18</sup>O – 4.3 ± 4.1). The groundwater and seep data indicated a winter season bias and a north–south latitudinal gradient, suggesting rapid near-surface groundwater flow without significant post-precipitation evaporation. Porewater isotope samples from unsaturated mine rock piles (MRPs) showed site-specific evaporation patterns, potentially due to convective air flows or exothermic sulphide oxidation. This research revealed the influence of groundwater and meltwater on rock drain discharge. Based on evaporative mass balance calculations, MRPs seasonally contributed ca. 5 %(December base flow) and 22 % (snowmelt) to drain discharge. The findings underscore the value of stable isotope data collections in the Elk Valley to help better define and quantify the hydrology–hydrogeology, including a better understanding of evaporative conditions in MRPs.
DOI: 10.6084/m9.figshare.27715455
title: Table S1

description: This study evaluates the environmental and CO₂ reduction potential of three microalgae-based products—biodiesel, fish feed, and biofertilizer—using a life cycle assessment across nine scenarios with varying electricity profiles (current Thai mix, 50% renewable hybrid, 100% renewable). Results showed that increased renewable energy use enhances the environmental benefits of all products. Fish feed, with a potential revenue of USD 560 million, had the lowest environmental impacts and could reduce CO₂ emissions by 1.1 million tonnes. Biodiesel could generate USD 3.5 billion and cut CO₂ emissions by 30 million tonnes, while biofertilizer could yield USD 2 billion, reducing CO₂ by 6 million tonnes. Collectively, these products could offset 37 million tonnes of CO₂, achieving 14% of Thailand's CO₂ reduction target and supporting national climate goals.
DOI: 10.15468/dl.j874fx
title: Occurrence Download

description: A dataset containing 748 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Oldenlandia capensis L.f."
 ]
}

 The dataset includes 748 records from 59 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013717-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3ddn4m
title: Occurrence Download

description: A dataset containing 10119 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is North America",
 "Country is Mexico",
 "DatasetKey is Flora de las playas y dunas costeras de México",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 10119 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013716-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.exfgf7
title: Occurrence Download

description: A dataset containing 1184 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hygrophila polysperma (Roxb.) T.Anderson"
 ]
}

 The dataset includes 1184 records from 77 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013718-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z8bqr6
title: Occurrence Download

description: A dataset containing 230142 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Lota lota (Linnaeus, 1758))",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Machine Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 230142 records from 171 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013711-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2853yb
title: Occurrence Download

description: A dataset containing 1829 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hygrophila ringens (L.) R.Br."
 ]
}

 The dataset includes 1829 records from 104 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013719-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wh6kvc
title: Occurrence Download

description: A dataset containing 337 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ompok bimaculatus (Bloch, 1794))",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Machine Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 337 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013720-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.