DOI: 10.5061/dryad.qv9s4mwq3
title: Data from: Inferring long-distance movements of insects using combined hydrogen isotope and genetic analyses: A case study of the African edible bush-cricket

description: Stable hydrogen (H) isotopes and mitochondrial COI sequence data can be
 used to investigate long-distance movements of species. We collected
 African bush-cricket (Ruspolia differens) specimens from Uganda, Kenya and
 Zambia from 2016 to 2020. We analysed the stable H isotope ratios from
 nymphal and adult wing chitin (specimens collected from 12 study regions).
 We used cytochrome c oxidase subunit I (COI) genetic sequences for
 population genetic analysis (specimens collected from 17 study regions).
 For our analyses, we recorded the specimen's sex, phase of
 development (nymph, non-swarming, or swarming), and collection date and
 region.
description: For detailed information on the methods, please consult our
 paper: Valtonen et al. Inferring long-distance movements of insects using
 combined hydrogen isotope and genetic analyses: a case study of the
 African edible bush-cricket. <em>Ecological Entomology</em>,
 https://doi.org/10.1111/een.13410.
description: # Data from: Inferring long-distance movements of insects using combined
 hydrogen isotope and genetic analyses: A case study of the African edible
 bush-cricket
 [https://doi.org/10.5061/dryad.qv9s4mwq3](https://doi.org/10.5061/dryad.qv9s4mwq3) ## Description of the data and file structure We collected African bush-cricket (*Ruspolia* *differens*) specimens from Uganda, Kenya and Zambia from 2016 to 2020. We analysed the stable H isotope ratios from nymphal and adult wing chitin (specimens collected from 12 study regions). We used cytochrome c oxidase subunit I (COI) genetic sequences for population genetic analysis (specimens collected from 17 study regions). For our analyses, we recorded the specimen's sex, phase of development (nymph, non-swarming, or swarming), and collection date and region. Individuals used in stable isotope and genetic analyses are different from each other. Please, consult our article Valtonen et al. Inferring long-distance movements of insects using combined hydrogen isotope and genetic analyses: a case study of the African edible bush-cricket. *Ecological Entomology*, [https://doi.org/10.1111/een.13410](https://doi.org/10.1111/een.13410) for details of the methods and analyses. This file describes the datasets used in the above article. The files consist of two .csv files containing: 1\)the stable hydrogen isotope data of *Ruspolia differens* and 2\)individuals included in the population genetic study. Individuals used in stable isotope and genetic analyses are different from each other. The methods used for obtaining the data and the terminology used in the data files are described in detail in the article (DOI: 10.1111/een.13410). The COI sequence data used in our work can be accessed in the BOLD Data Portal ([www.boldsystems.org/](http://www.boldsystems.org/))  using individual Bold ID access codes (see the genetic_study_rdifferens.csv file). ### Files and variables #### File: genetic\_study\_rdifferens.csv **Description:** *Ruspolia differens* individuals (non-swarming and swarming adults) included in the population genetic study. Please consult our paper for detailed definitions of the terms and methods used. ##### Variables * Bold ID: code for [BOLD Data Portal](https://www.boldsystems.org/) to access specimens COI sequence and other specimen information. * Country: country, where the specimen was collected (Uganda, Kenya, or Zambia) * Study region: municipality or district, where the specimen was collected * Non-swarming/swarming: describes which developmental phase (non-swarming or swarming) the individual exhibited when collected * Sex: specimen's sex (male or female) based on morphology * CollectionDay: day of collection * CollectionMonth: month of collection * CollectionYear: year of collection Missing data codes: NA (data not available). #### File: h\_isotopes\_rdifferens.csv **Description:** Stable H (hydrogen) isotope data of *Ruspolia differens* individuals. Please consult our paper for detailed definitions of the terms and methods used. ##### Variables * Country: country, where the specimen was collected (Uganda, Kenya, or Zambia) * Study region: municipality or district, where the specimen was collected * Non-swarming/swarming/nymph: describes which developmental phase (non-swarming or swarming) the individual exhibited when collected * Sex: specimen's sex (male or female) based on morphology * CollectionDay: day of collection * CollectionMonth: month of collection * CollectionYear: year of collection * d 2Hn: the stable H isotope ratio (delta per mil) of the sample (*R. differens* wing chitin) to the international reference ## Code/software The .csv files can be viewed with any spreadsheet program. ## Access information Other publicly accessible locations of the data: * The COI sequences can be accessed in [BOLD Data Portal](https://www.boldsystems.org/) using the 'Bold ID' code in genetic_study_rdifferens.csv file.