DOI: 10.5061/dryad.5x69p8dj6
title: Data from: Reversible plasticity in the communication system of the cricket <em>Gryllus rubens</em>

description: Animals display phenotypic plasticity that can lead to the evolution of
 novel traits. The evolutionary potential of plasticity is heightened if
 traits are involved that affect reproduction. It is typically not known if
 phenotypic changes due to plasticity are permanent or reversible. I tested
 whether the plastically induced changes in mating songs and song
 preferences of the cricket, Gryllus rubens were permanent or reversible. I
 reared males and females as juveniles until adulthood at one temperature
 (‘cool’ or ‘warm’), recorded males and tested female preferences for the
 songs, and then kept the animals for one week at the other temperature
 (‘warm’ or ‘cool’) and rerecorded males and retested females to test if
 the initially induced songs and preferences changed after exposure to the
 other temperature. The pulse rate of male songs increased significantly
 after increasing the rearing temperature from ‘cool’ to ‘warm’ and
 decreased significantly when decreasing the temperature from ‘warm’ to
 ‘cool’. Female preference changed significantly, and in parallel with the
 songs, i.e., female attraction for the ‘warm’ song increased, and that of
 the cool song decreased after increasing the rearing temperature from
 ‘cool’ to ‘warm’ for one week. Females displayed the opposite attraction
 pattern to the stimuli when reducing the temperature from ‘warm’ to
 ‘cool’. The here deposited files provide the raw data of the song
 recordings and female preferences and the applied statistical analyses of
 these data.
description: # Data from: Reversible plasticity in the communication system of the
 cricket *Gryllus rubens* Dataset DOI:
 [10.5061/dryad.5x69p8dj6](https://doi.org/10.5061/dryad.5x69p8dj6) ##
 Description of the data and file structure Oliver M Beckers Contact:
 [obeckers@murraystate.edu](mailto:obeckers@murraystate.edu) Explanation of
 used data sets. 'Female preference data.xlxs' contains the data
 from the female phototaxis trials before and after switching the rearing
 temperatures and 'male song data' those of the male calls
 recorded before and after switching rearing temperatures. ### Explanation
 of 'Female_preference_data-final.xlsx' tables Tabs: 'warm
 to cool': females were reared in 'warm' condition until
 first set of behavioral tests, and then transferred to 'cool'
 conditions before tested again with the same two song stimuli. 'Cool
 to warm': Same as before but reared 'cool' first and then
 transferred to 'warm' rearing conditions. Data sheets:
 'warm to cool' and 'cool to warm': each row is data
 from the same animal. Columns in each data sheet: * animal: ID number of
 individual tested. * test1: First set of trials. * circlecool1: Time spent
 within loudspeaker circle in response to 'cool' stimulus in the
 first set of trials. Time in seconds. * circlewarm1: Time spent within
 loudspeaker circle in response to 'warm' stimulus in the first
 set of trials. Time in seconds. * exposure1: Time as adult exposed to
 first rearing temperature. Exposure in days. * test2: Second set of
 trials. * circlecool2: Time spent within loudspeaker circle in response to
 'cool' stimulus in the second set of trials. Time in seconds. *
 circlewarm2: Time spent within loudspeaker circle in response to
 'warm' stimulus in the second set of trials. Time in seconds. *
 exposure2: Time exposed to second rearing temperature. Exposure in days.
 ### Explanation of 'Male_song_data-final.xlsx' tables Tabs:
 'warm to cool': males were reared in 'warm' condition
 until first recording, and then transferred to 'cool' conditions
 before recorded again. 'Cool' to warm': Same as before but
 reared 'cool' first and then transferred to 'warm'
 rearing conditions. Columns in each data sheet: * individual: ID number of
 individual tested. * treatment: 1= 'cool to warm' temperature
 order, 2= 'warm to cool' temperature order. * file1: Id of first
 song recording * pulse.duration: average pulse duration of analyzed first
 recorded song. Time in ms. * interval.duration: average interval duration
 of analyzed first recorded song. Time in ms. * pulse.rate: average pulse
 rate of analyzed first recorded song. Rate in pulses/s. * exposure.1: Time
 as adult exposed to first rearing temperature. Exposure in days. * file2:
 Id of second song recording. * pulse.duration2: average pulse duration of
 analyzed second recorded song. Time in ms. * interval.duration2: average
 interval duration of analyzed second recorded song. Time in ms. *
 generation: generation of individual male. * pulse.rate2: average pulse
 rate of analyzed second recorded song. Rate in pulses/s. * exposure.2:
 Time exposed to second rearing temperature. Exposure in days. ###
 Explanation of file 'ReversiblePlasticityModels.R' This file
 contains the models used for analysis of the male call and female
 preference data. ## Code/software To run the statistical analysis, install
 R (version 4.4.2) and the package lme4 (lmer function) was used for
 analyses. ## Access information NA