DOI: 10.5061/dryad.x69p8czrf
title: Data from: Seasonal diet partition among top predators of a small island, Iriomotejima island in the Ryukyu Archipelago, Japan

description: In general, small islands lack predators because species at higher trophic
 levels often cannot survive. However, two predators—the Iriomote cat
 Prionailurus bengalensis iriomotensis, and the Crested Serpent Eagle
 Spilornis cheela perplexus—live on Iriomotejima Island in the Ryukyu
 Archipelago, which covers an area of approximately 284 square kilometers.
 To understand how these two top predators coexist on such a small island
 with limited resources, we focused on their seasonal feeding habits which
 are considered crucial for survival in such an island ecosystem. To
 compare the diets of the Iriomote cat and Crested Serpent Eagle, we used
 DNA metabarcoding analysis of their fecal samples. In the summer, we
 identified 16 prey items from Iriomote cat fecal samples, and 15 Crested
 Serpent Eagle fecal samples. In the winter, we identified 37 and 14 prey
 items, respectively. Using a non-metric multidimensional scaling (NMDS)
 and a permutational multivariate analysis of variance (PERMANOVA), our
 study reveals significant differences in the diet composition at the order
 level between the predators during both seasons. Furthermore, although
 some prey items at the species-to-order level overlapped between the two
 predators, the frequency of occurrence of most prey items differed between
 them in both seasons. These results suggest that this difference in diets
 was one of the reasons why the Iriomote cat and the Crested Serpent Eagle
 coexisted on such a small island.
description: # Data from: Seasonal diet partition among top predators of a small
 island, Iriomotejima island in the Ryukyu Archipelago, Japan
 This folder contains the
 data, computer code, and document files for the article. Please address
 any comments or requests to Alisa Tobe at .
 All materials are free to use, but I would appreciate being notified and
 the dataset and matching paper cited if appropriate. ## Description of the
 data and file structure "no_sequencing_reads_primer name.csv"
 contains the number of sequencing reads for each prey taxon in each fecal
 sample from each primer set after taxonomic profiling. These data were
 used to calculate the values described in Figure 3.
 "presence_absence_taxonomic level.csv" includes the
 presence/absence data of each prey taxon integrated with all primer
 information at each taxonomic level. "metrix.pdf" shows how to
 calculate FOO and wPOO using above csv files.
 "presence_absence_class.csv" level was used to calculate the
 values descripted in Figure 4, 5, 7, 8, and Table S2.
 "rf_rarefaction_species_season.csv" contains data on the
 presence/absence of each prey order for each species during each season.
 "R-script#1.txt" demonstrates how to plot the accumulation and
 extrapolation curve described in Figure S1 using
 "rf_rarefaction_species_season.csv".
 "rf_nmds_season.csv" includes data of the presence/absence of
 each prey order for each species during each season.
 "R-script#2.txt" demonstrates how to analyze the NMDS and
 PERMANOVA descripted in Figure 6 using "rf_nmds_season.csv". ##
 Sharing/Access information The MiSeq-generated raw sequence reads from
 this study can be found in the DDBJ Sequence Read Archive (DRA) under the
 accession numbers DRA017514. The link is below: *
 ##
 Software All statistical analyses were performed using *R* version 4.3.0
 (*R* Core Team 2023).