cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j00z7211
title: CIL:13915, Homo sapiens, permanent cell line cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue; cultured cells in vitro
Item type: recorded image; charge coupled device (CCD)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; Red fluorescent proteins from Anthozoa
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation
description: Image indicative of 24 h autophagic activity of human colon carcinoma HCT 116 cell co-transfected with a RFP-LC3-encoding plasmid and a SIRT1 fused to GFP plasmid. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8C, middle panel, in Morselli et al. J Cell Biol 192: 615-629
DOI: 10.6075/j0xp73n4
title: CIL:13917, Mus musculus, hepatocyte. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; embedded tissue; cryostat-sectioned tissue
Relation to intact cell: sectioned tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation
description: Transgenic C57BL/6 mice expressing GFP-LC3 fusion protein, uninjected as control for injection with Resveratrol and/or spermidine. To avoid postmortem autophagy induction, dead mice were immediately perfused with 4% paraformaldehyde (wt/vol in PBS, pH 7.4). Tissues were then harvested and further fixed with the same solution for ≥4 h followed by treatment with 15% sucrose (wt/vol in PBS) for 4 h and with 30% sucrose (wt/vol in PBS) overnight. Tissue samples were embedded in Tissue-Tek OCT compound (Sakura) and stored at −70C. 5-µm-thick tissue sections were generated with a cryostat (CM3050 S; Leica), air dried for 1 h, washed in PBS for 5 min, dried at RT for 30 min, and mounted with Vectashield antifading medium. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 40× 1.15 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 9C, bottom left panel (liver/Co), in Morselli et al. J Cell Biol 192: 615-629
DOI: 10.6075/j0xg9pvj
title: CIL:13912, Homo sapiens, permanent cell line cell. In Cell Image Library

description: Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with 100 µM Resveratrol for 4 h. Cytoplasts were still able to accumulate GFP-LC3 puncta in response to spermidine or resveratrol treatment, indicating that nuclei (and by extension transcription) are not required for short-term autophagy stimulation by these two agents. Nuclei were counterstained by Hoechst. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8A, middle panel, in Morselli et al. J Cell Biol 192: 615-629
description: Preparation: isolated subcellular component
Relation to intact cell: enucleated cell
Item type: recorded image; charge coupled device (CCD)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization methods: EGFP; membrane-permeant probe; Hoechst
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation
DOI: 10.6075/j0cv4gd5
title: CIL:13911, Homo sapiens, permanent cell line cell. In Cell Image Library

description: Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with vehicle (control for Resveratrol and Spermidine experiments). Nuclei were counterstained by Hoechst. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8A, left panel, in Morselli et al. J Cell Biol 192: 615-629
description: Preparation: isolated subcellular component
Relation to intact cell: enucleated cell
Item type: recorded image; charge coupled device (CCD)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization methods: EGFP; membrane-permeant probe; Hoescht
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation
DOI: 10.6075/j0611058
title: CIL:36984, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

description: This is IMAGE #2 (day 2) of a time lapse series contained with in this image group. It shows 2 developing neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. 
 
 The images were acquired daily over 3 days and show dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions.
 
 The cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.
description: Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in: 
Ruthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603. 

The methods of cell transfection with electroporation can be found here: 
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272. 

Further explanation can be found here:
Bestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.

Bestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EYFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.48719/belab.ide1325_01
title: Foreign Direct Investment Microdata (IDE)

description: BELab provides the following Foreign Direct Investment data: assets and liabilities (stocks, transactions and other changes in volume) of direct investment in listed equities, unlisted equities and other equity, loans and other debt.<br><br><br>Universe: population of Spanish resident investors in non-resident companies and non-resident investors in Spanish resident companies as long as they have a stake equal to or greater than 10% in the capital of the direct investment company (the one receiving the investment), also included are all sister companies and mutual cross-investments of subsidiaries in their parents.<br><br>Geographic Coverage: IDE assets: Direct investors resident in Spain and direct investment companies of any country except Spain. <br>IDE liabilities: Direct investors of any country and direct investment enterprises resident in Spain. <br><br>Time Coverage: 2013-12-31/2025-06-30<br><br>Sampling Methods: <br><br>Availability: OnSite/Remote<br><br>Frequency: Annual<br><br>
DOI: 10.6075/j02z147w
title: CIL:13899, Saccharomyces cerevisiae S288c. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation
description: Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) undergoing chronological aging on small synthetic 2% glucose media (SMD) with supplementation of 4 mM spermidine. Spermidine was added to stationary cultures at day 1 of the aging experiments. Image represents EGFP-Atg8p localization, visualized by fluorescence microscopy. Yeast cells undergoing autophagy exhibit a prominent vacuolar localization of EGFP-Atg8p. Epifluorescence image was captured using a widefield microscope (Axioskop; Carl Zeiss, Inc) fitted with a Plan Neofluar 63× 1.25 NA oil immersion objective. Image acquired with a camera (SPOT 9.0 Monochrome 6; Diagnostic Instruments, Inc) using Metamorph software (Universal Imaging Corp) and processed with IrfanView and Photoshop (CS2; Adobe) software. Specifically, picture processing involved coloring and cropping of representative areas and was performed with IrfanView. In addition, linear adjustments of contrast and brightness were applied with Photoshop (using equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 2A, WT/Spd/EGFP-Atg8p panel, in Morselli et al. J Cell Biol 192: 615-629
DOI: 10.6075/j0kd1wm2
title: CIL:13898, Saccharomyces cerevisiae S288c. In Cell Image Library

description: Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0), transformed with EGFP-Atg8p, undergoing chronological aging on small synthetic 2% glucose media (SMD) with supplementation of 4 mM spermidine. Spermidine was added to stationary cultures at day 1 of the aging experiments. Image represents yeast morphology, visualized by DIC. DIC image was captured using a widefield microscope (Axioskop; Carl Zeiss, Inc) fitted with a Plan Neofluar 63× 1.25 NA oil immersion objective. Image acquired with a camera (SPOT 9.0 Monochrome 6; Diagnostic Instruments, Inc) using Metamorph software (Universal Imaging Corp) and processed with IrfanView and Photoshop (CS2; Adobe) software. Specifically, picture processing involved coloring and cropping of representative areas and was performed with IrfanView. In addition, linear adjustments of contrast and brightness were applied with Photoshop (using equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 2A, WT/Spd/DIC panel, in Morselli et al. J Cell Biol 192: 615-629
description: Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Processing history: brightness and contrast adjusted
Data qualification: Processed
DOI: 10.15468/dl.5vd7s7
title: Occurrence Download

description: A dataset containing 146709 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Crocodylus"
 ]
}

 The dataset includes 146709 records from 280 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009618-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nmjwe8
title: Occurrence Download

description: A dataset containing 98 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Wollastonia biflora (L.) DC.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 98 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0192549-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fhmccp
title: Occurrence Download

description: A dataset containing 37 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Abelmoschus moschatus (L.) Medik.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 37 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198448-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.h2cv2e
title: Occurrence Download

description: A dataset containing 8846991 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is United States of America",
 "DatasetKey is iNaturalist Research-grade Observations",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Tracheophyta",
 "Year 2018-2023"
 ]
}

 The dataset includes 8846991 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0244859-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.82fdk5
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Gossypium hirsutum L.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198452-220831081235567/datasets/export for details.
DOI: 10.15468/dl.8uk3cb
title: Occurrence Download

description: A dataset containing 57 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Clematis courtoisii Hand.-Mazz."
 ]
}

 The dataset includes 57 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0247527-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.btkmst
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Malvastrum americanum (L.) Torr.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198455-220831081235567/datasets/export for details.
DOI: 10.15468/dl.dhtfdk
title: Occurrence Download

description: A dataset containing 2620 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Polystichum tsus-simense (Hook.) J.Sm."
 ]
}

 The dataset includes 2620 records from 105 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0248021-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.e9tuce
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cuphea cyanea Moc. & Sessé ex DC.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198457-220831081235567/datasets/export for details.
DOI: 10.15468/dl.bmwbnu
title: Occurrence Download

description: A dataset containing 59 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "Country is one of (Guyana, Peru, French Guiana, Bolivia (Plurinational State of), Ecuador, Venezuela (Bolivarian Republic of), Brazil)",
 "DatasetKey is one of (Bee Biology and Systematics Laboratory, Entomology Division, Yale Peabody Museum, Snow Entomological Museum Collection)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Eufriesea pulchra (Smith, 1854)"
 ]
}

 The dataset includes 59 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0250521-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.a5bbfc
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Melastoma sanguineum Sims",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198462-220831081235567/datasets/export for details.
DOI: 10.15468/dl.a6h4du
title: Occurrence Download

description: A dataset containing 40864 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Observation, Machine Observation, Human Observation, Occurrence evidence)",
 "CoordinateUncertaintyInMeters 0.0-1000000.0",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "IucnRedListCategory is CR",
 "OccurrenceStatus is Present",
 "TaxonKey is Plantae",
 "Year 1970-2022"
 ]
}

 The dataset includes 40864 records from 210 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0229778-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3bvfkv
title: Occurrence Download

description: A dataset containing 100 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aster glehnii F.Schmidt",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 100 records from 19 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0192547-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.96mr6v
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Epilobium fauriei Lév.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 21 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198463-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.u7ewfp
title: Occurrence Download

description: A dataset containing 285028 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Phalaropus tricolor (Vieillot, 1819)",
 "Year 2010-2022"
 ]
}

 The dataset includes 285028 records from 39 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0229783-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z9tbsm
title: Occurrence Download

description: A dataset containing 324 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Cymbaria daurica L."
 ]
}

 The dataset includes 324 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0247529-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.uhxymt
title: Occurrence Download

description: A dataset containing 30668520 species occurrences available in GBIF matching the query:
{
 "Continent" : [
 "is Africa"
 ]
}

 The dataset includes 30668520 records from 1099 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0244865-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.txx97z
title: Occurrence Download

description: A dataset containing 300 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Observation, Machine Observation, Human Observation, Material sample, Material citation, Specimen, Living Specimen, Occurrence evidence)",
 "Country is New Zealand",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Metrosideros carminea W.R.B.Oliv."
 ]
}

 The dataset includes 300 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198466-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.khsc8e
title: Occurrence Download

description: A dataset containing 377 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lycocerus Gorham",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 377 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0192558-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.xpk8cm
title: Occurrence Download

description: A dataset containing 90 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pemphis acidula J.R.Forst. & G.Forst.",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 90 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198458-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.