DOI: 10.5061/dryad.d2547d872
title: Phylogenetic analysis of the new species Dictyanthus reflexiflorus

description: A new species of Dictyanthus from the state of Michoacán, Mexico, is
 described and illustrated: Dictyanthus reflexiflorus sp. nov. It grows in
 tropical deciduous forest located in the municipality of Tacámbaro. A
 phylogenetic analysis based on molecular data (plastid regions trnL-trnF
 and rps 16) was conducted to explore the relationships of the new species.
 Results place the new species in Dictyanthus, congruent with its
 morphology. Characters that support this relationship are the presence of
 uncinate hairs, a reticulate corolla, and a gynostegial corona basally
 fused to the stipe of the gynostegium and partially or completely fused to
 the corolla tube. The species has unique characteristics within the genus,
 such as a reflexed corolla and the presence of slits along the corolla
 lobes. A key to distinguish Dictyanthus reflexiflorus from congeners in
 the state of Michoacán is included.
description: <strong>Phylogenetic analysis</strong>—To assess the
 phylogenetic position of <em>Dictyanthus</em>, we employed
 sequences which had been used successfully in previous phylogenetic
 studies of Gonolobinae (Liede-Schumann et al. 2005; Krings et al. 2008;
 Mangelsdorff et al. 2016). The sequences were aligned separately using
 MAFFT v. 7 (Katoh and Standley 2013) with FFT–NS–i iterative refinement
 method, and the rest of the parameters selected by default. Subsequently,
 the alignments were manually edited using PhyDE v. 0.9971
 (Müller et al. 2010). For the substitution model of
 each marker, we used Model Finder (Kalyaanamoorthy et al. 2017) employing
 default parameters and chosen under Akaike information criteria (AIC).
 Maximum Likelihood Analysis (ML) was performed in IQ-TREE web server
 (Trifinopoulos et al. 2016) with ultrafast bootstrapping (Hoang
 <em>et al.</em> 2018) of 1000 replicates and the rest of
 parameters selected by default. Consensus tree and bootstrap (BS) were
 visualized and edited in Fig Tree v. 1.4.3 (Rambaut 2016). Bayesian
 Inference (BI) was performed using Mr. Bayes v. 3.2.6 (Ronquist et
 al.<em> </em>2012) with two independent runs and four Markov
 chains Monte Carlo (MCMC) each. Each chain ran for 10 million generations
 and was sampled every 1,000 generations. The convergence of the chains was
 checked using Tracer v. 1.7.1 (Rambaut et al.<em> </em>2018),
 and according to the performance of the chains, 25% of the trees were
 eliminated as burn-in. The remaining topologies were used to build a
 majority rule consensus tree, which was viewed using FigTree v. 1.4.3
 (Rambaut 2016).
description: # Dataset of the new species Dictyanthus reflexiflorus --- The dataset
 contains the alignment of markers rps16, trnL and the trnL-trnF spacer for
 the subtribe Gonolobinae. In addition to the phylogenies resulting from ML
 and BI analyses. ## Description of the data and file structure The
 alignment in NEXUS format was used to obtain the ML and BI trees in .tree
 files. ## Sharing/Access information Most of the sequences were obtained
 through GenBank, for accession numbers see: (Lozada-Prez et al. 2023). The
 sequences were aligned separately using MAFFT v. 7, Maximum Likelihood
 Analysis was performed in IQ-TREE web server and Bayesian Inference was
 performed using Mr. Bayes v. 3.2.6