DOI: 10.5061/dryad.2z34tmpr5
title: Data from: House mouse Slx and Sly CNV and expression data

description: A remarkable gene copy number (CN) arms race system has recently been
 described in laboratory mice, where Slx;Slxl1 and Sly genes compete over
 transmission by altering fertilisation success of X and Y
 chromosome-bearing sperm, respectively. Here, we focus on this system in
 nature, where natural selection can counter CN/gene product escalation.
 Our model is house mouse subspecies hybridising in Europe. In some
 regions, Y chromosomes of the Eastern subspecies have introgressed onto
 Western genomic backgrounds, accompanied by sex ratio distortion in favour
 of males, consistent with the inbred-lines suggested mechanism:
 overabundance of SLY protein expressed by invading Y chromosomes. We take
 Slx as representative of the X side of this arms race and measure Slx|Sly
 CN and expression across an ‘Invasion’ transect where Ys introgress and a
 ‘Control’ transect with negligible introgression. Since we found similar
 Slx|Sly ratios in both transects, SLY overabundance is unlikely to explain
 the introgression. However, Slx CN is relatively low in the introgression
 area, suggesting Slx is less able to combat Sly effects here. Further,
 deterministic changes in Slx;Sly expression proportions versus CN
 proportions suggest standing variation for trans regulation of Slx|Sly is
 being co-opted in nature where their arms race reduces population fitness.
description: Sampling of the Brandenburg transect was carried out in
 cooperation with E. Heitlinger of the Humboldt University Berlin. Primary
 steps of ddPCR optimising were done in collaboration with D. Tautz during
 the stay of Z.H. at the Max-Planck Institute for Evolutionary Biology
 (Plön, Germany), with the help of A. Yanchukov and N. Thomsen. We also
 acknowledge CF Genomics CEITEC, Masaryk University (Brno, Czech Republic),
 for their support while generating all individual CN estimates presented
 in this paper. In total, we analysed 252 male mice
 live-trapped in two distinct transect regions across the HMHZ, the first
 one stretching from north-eastern Bavaria (Germany) to western Bohemia
 (Czech Republic) where Eastern Y chromosomes introgress across the zone
 deep into the Western territory (hereafter ‘Invasion transect’; N = 180)
 and the other situated in north-eastern Brandenburg (Germany) with no or
 negligible introgression (hereafter ‘Control transect’; N = 72) (Figure
 1). Most of the captured males were dissected immediately in a field
 laboratory, whereas others were dissected in External Research Facility
 Studenec, IVB. One testis and the spleen were either placed in ethanol
 (when only CNV was being estimated) or snap-frozen in liquid nitrogen
 immediately after dissection and then stored at – 80 °C until use (when
 both CNV and expression were measured). Total DNA and RNA were extracted
 using AllPrep DNA/RNA Mini Kit (Qiagen GmbH, Hilden, Germany). DNeasy® 96
 Tissue Kit (Qiagen) and Invisorb® Spin DNA Extraction Kit (Stratec
 Biomedical AG, Birkenfeld, Germany) were used for DNA extraction from
 ethanol-preserved samples (CNV analysis only). All the procedures followed
 the manufacturers’ instructions.