cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14307155.v1
title: Nitrogen rates on the agronomic performance of second-crop corn single and intercropped with ruzigrass or showy rattlebox

description: ABSTRACT Topdressing nitrogen (N) rates may alter the second-crop corn performance, and this response may vary if the corn is grown single or intercropped with grass or leguminous species. This study aimed to evaluate the effect of topdressing N rates on the growth and productive performance of second-crop corn grown single or intercropped with ruzigrass (Urochloa ruziziensis) or showy rattlebox (Crotalaria spectabilis). The experiment was carried out in a randomized block design, with a split-plot arrangement and four replications. The plots consisted of corn grown single or intercropped with ruzigrass or showy rattlebox, while the subplots consisted of N rates (0, 60, 120, 180 and 240 kg ha-1). The evaluated variables were: SPAD index at R1; normalized difference vegetation index at V4 and V7; leaf area at R1; leaf and total corn dry mass at R1; grain and total corn dry mass at R6; number of ears per plant and grains per ear; 1,000-grain mass; and grain yield. In the absence of N fertilization and with topdressing of 60 kg ha-1 of N, the intercropping with U. ruziziensis reduces the growth and productive performance of second-crop corn. The topdressing N fertilization for second-crop corn intercropped with U. ruziziensis minimizes the forage competition on corn growth and provides a productive performance similar to monocropping from 120 kg ha-1 of N. The intercropping with C. spectabilis does not change the corn growth and productive performance, as well as the response to the topdressing N rates, when compared to monocropping, in the second crop cultivation.
DOI: 10.6084/m9.figshare.14307152.v1
title: Manganese fertilization for sweet cassava production under organic management system

description: ABSTRACT Organic management farms in the Federal District, Brazil, usually present overlimed and overfertilized soils, with high content of organic matter and high pH. These conditions lead to a low availability of manganese to crops. Many sweet cassava (Manihot esculenta Crantz.) crops were diagnosed with Mn deficiency in the region, presenting low yields. In order to overcome such condition, an experiment was established comprising the following treatments: application of MnSO4 banded in rows; treatment of cassava cuttings with a MnSO4 solution before planting; application of MnSO4 and elemental sulfur (S0) banded in the rows, to acidify the soil, increasing the Mn solubility; and leaf spraying of a Mn solution. The best results were revealed when the Mn was applied by foliar spraying and using a combination of methods (treatment of cassava cuttings with Mn, and Mn and S0 applied to the soil before planting). This treatments provided a fresh root yield of 19.5 Mg ha-1, contrasting with the control treatment (3.6 Mg ha-1).
DOI: 10.6084/m9.figshare.14307149.v1
title: Agro-economic performance of bur gherkin under staking systems

description: ABSTRACT The bur gherkin cultivation is predominantly carried out without a staking system, but problems such as the "white belly" and fruit rot occur in this system. This study aimed to evaluate staking systems for bur gherkin plants. The experimental design was randomized blocks, with five treatments (no staking, agricultural netting staking, Mexican staking, vertical ribbon with total secondary stem thinning and vertical ribbon with alternate secondary stem thinning) and four replicates. The fresh mass and number of fruits per plant, fruit length and average diameter, number and fresh mass of fruits with white belly per plant, fresh mass per fruit and total yield were evaluated, as well as the economic indicators gross income, net income, rate of return and profitability index. For all the staking systems, the incidence of fruits with white belly was lower. The agricultural netting (43.75 t ha-1), Mexican (35.94 t ha-1) and vertical ribbon with alternate secondary stem thinning (22.24 t ha-1) systems promoted yields superior to that for no staking. The best economic indicator (US$ 21,031.46 ha-1) was observed for the agricultural netting staking, which is the most recommended for the bur gherkin cultivation.
DOI: 10.6084/m9.figshare.14307147.v1
title: Genetic divergence and path analysis in wheat cultivars under heat stress

description: ABSTRACT One of the biggest limitations for the wheat crop expansion to Brazilian tropical regions is the high temperature in the rainy season. This study aimed to select, based on genetic divergence and path analysis, cultivars that tolerate cultivation in the summer (heat stress). Nine wheat cultivars were sown, using a randomized block design with three replications, with plots consisting of 10 rows of 6 m and row spacing of 0.15 m. The highest grain yield and number of ears m-2 were observed for the CD 150 cultivar and the largest 1,000-grain weight for CD 116. BRS 220, IPR 136, IPR 144 and BRS Pardela had the highest number of sterile spikelets. The highest number of grains per ear was observed for CD 150, CD 116, BRS Pardela, IPR 130 and IPR 85, showing direct effects on grain yield, evidenced by the path analysis. CD 116 and CD 150 were the most productive cultivars and can be considered as possible parents in wheat breeding programs. IPR 85, CD 116, CD 108 and CD 150 showed a greater tolerance to high temperatures.
DOI: 10.6084/m9.figshare.14307146.v1
title: Neoseiulus californicus preying on Tenuipalpus heveae

description: ABSTRACT The spread of Tenuipalpus heveae Baker (Acari: Tenuipalpidae) can be controlled by managing predatory species such as Neoseiulus californicus (McGregor) (Acari: Phytoseiidae). This study aimed to evaluate the predation of N. californicus at different biological phases on T. heveae at different development stages. The experiments were carried out under laboratory-controlled conditions, counting the number of predated individuals by development stage after 24, 48 and 72 h. N. californicus, in all phases, consumed T. heveae, with a higher consumption of nymphs and larvae by adult predators. Both sexes of adult predators exhibited predation efficiency, but females showed a greater acceptance of adult preys than males. The daily rate of adult predation decreased over time; however, the highest consumption took place in the first 24 h.
DOI: 10.6084/m9.figshare.14307143.v1
title: Yield and profitability of flooded rice genotypes in relation to nitrogen doses and phosphorus and potassium application

description: ABSTRACT The use of hybrid genotypes is a good alternative for increasing the flooded rice yield. However, there is a need for validation of the fertilizer management under different edaphoclimatic conditions and its greater profitability. This study aimed to evaluate the grain yield and profitability of rice hybrids in relation to N doses and fertilization with P and K. Five hybrid cultivars and the conventional IRGA 424 CL cultivar combined with four N doses (0, 100, 150 and 200 kg ha-1) were evaluated, all with fixed doses of 90 kg ha-1 of P2O5 and 150 kg ha-1 of K2O, besides an additional treatment without the addition of fertilizers. The N application increases, on average, by 10 and 35 % the flooded rice yield in years with favorable and unfavorable climatic conditions, respectively. Regardless of the year and genotype, the highest yields are obtained with doses of N between 106 and 200 kg ha-1, with the most profitable dose being the 150 kg ha-1 one (22 % higher than for the absence of N). The hybrids produce an average of 20 % more grains than the IRGA 424 CL cultivar, increasing the profitability by 18 %. In soil with adequate P and K availability, there is no increase in yield due to the application of these nutrients. However, in deficient soil, the fertilization with P and K increases the grain yield by 21 %.
DOI: 10.6084/m9.figshare.14307141.v1
title: Management of liquid swine manure: impact on mineral nitrogen dynamics and corn yield

description: ABSTRACT The agronomic efficiency of liquid swine manure (LSM) depends on the use of strategies to mitigate nitrogen (N) losses after its application to the soil, and thus preserve its fertilizing potential. In order to evaluate the injection of LSM into the soil and the addition of a nitrification inhibitor, as ways to increase its efficiency as a N source to the corn crop in the no-tillage system, an experiment was carried out under an Aluminofferic Red Latosol. The used treatments were: one control, surface application (total area) of LSM, injection of LSM in furrows, surface application (total area) of LSM + dicyandiamide (DCD), injection of LSM + DCD in furrows, and mineral fertilization. The mineral N in the soil and number of rows per ear, number of grains per row, 1,000-grain mass and grain yield for corn were evaluated. One day after the application of the treatments, the recovery of the mineral N in the soil with the injection of LSM exceeded by 10 kg ha-1 that observed with the superficial application. With DCD, the recovery of anionic forms (NO2- and NO3-) in the soil was lower in the first 20 days after the application, with the change in the N dynamics caused by DCD resulting in a higher content of mineral N at 30 days after sowing. The transformation rate for the anionic forms of mineral N in the soil is reduced with the addition of DCD. The injection of LSM with DCD does not differ from mineral fertilization, in terms of corn grain yield.
DOI: 10.6084/m9.figshare.14307140.v1
title: Macronutrient accumulation curves in potato genotypes in the Brazilian Savanna

description: ABSTRACT Knowledge on the nutritional demands of different potato genotypes is necessary for the development of fertilizing recommendations. This study aimed to determine and compare the macronutrient accumulation curves in the Agata, Asterix, BRSIPR Bel and F183-08-01 potato genotypes, in order to subsidize the split fertilization that could meet the specific demands of each genotype. A randomized block design was used, with the treatments (genotypes × plant stages) arranged in a split-plot scheme, with three replications. The genotypes were grown in plots containing 75 plants each. The subplots within a plot contained four plants each and corresponded to the number of days after planting, when destructive samples of whole plants were collected. The plants from the subplots were harvested at 24, 38, 52, 66 and 80 days after planting, to estimate the dry mass accumulated by different parts of the plant (shoots + roots, tubers and whole plants), as well as to determine the macronutrient accumulation curves. F183-08-01 presented the highest total accumulation of N, Ca, Mg and S (141.37, 29.21, 25.16 and 22.36 kg ha-1, respectively). The highest rates of macronutrient accumulation for all the potato genotypes occurred at 24 days after planting, coinciding with the beginning of the tubers formation.
DOI: 10.6084/m9.figshare.14290794.v1
title: Development and validation of a HPTLC method for analysis of Sunitinib malate

description: ABSTRACT A simple high performance thin layer chromatography (HPTLC) has been developed and validated for determination of sunitinib malate and possible impurities. The samples were applied in forms of bands on an aluminum TLC plate pre-coated with silica gel and were separated using dichloromethane: methanol: toluene: ammonia solution as the mobile phase. Sunitinib malate was thoroughly separated from impurities including E-isomer, sunitinib N-oxide and impurity B with a retention factor (RF) of 0.35±0.02. Quantitative analysis of sunitinib was carried out using a mobile phase consisting of dichloromethane:methanol:ammonia solution, RF value was 0.53±0.02 for Z isomer. Detection was performed densitometrically in absorbance mode at 430 nm. This method was found to produce sharp, symmetrical, and well resolved peaks. Linear relationship with the coefficients of determination &gt; 0.99 was achieved over the concentration range of 27.34 to 437.5 ng/spot. This method provides robust, replicable and accurate results with acceptable sensitivity.
DOI: 10.6084/m9.figshare.6272807
title: Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis

description: ABSTRACT Our present investigation deals with the phytochemical screening, estimation of total flavonoids, terpenoids and tannin contents to evaluate the anti-diabetic activities of Salacia oblonga stem followed by GC-MS analysis. It explores the natural compounds and the potential α-amylase and α-glucosidase inhibitory actions of stem extracts. The aqueous stem extract was selected from other extracts (ethanol, acetone, petroleum ether and chloroform) for the in vitro study of anti-diabetic activity by alpha amylase and alpha glucosidase inhibitory assays. The stem extract was also analyzed by gas chromatography mass spectrometry to identify the natural chemical components. Phytochemical analysis of aqueous stem extract showed major classes of secondary metabolites such as phenols, flavonoids, alkaloids, terpenoids, tannins, saponins. The total flavonoid, terpenoid, and tannin contents were quantified as 19.82±0.06 mg QE/g, 96.2±0.20 mg/g and 11.25±0.03 mg TAE/g respectively. The percentage inhibition of assays showed maximum inhibitory effects (59.46±0.04% and 68.51±0.01%) at a concentration of 100 mg/mL. The IC50 values of stem extract was found to be 73.56 mg/mL and 80.90 mg/mL for alpha amylase and alpha glucosidase inhibition. Fifteen chemical constituents were found by GC-MS analysis. This study suggest the aqueous stem extract of Salacia oblonga might be considered as potential source of bio active constituents with excellent antidiabetic activity.
DOI: 10.6084/m9.figshare.6272807.v1
title: Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis

description: ABSTRACT Our present investigation deals with the phytochemical screening, estimation of total flavonoids, terpenoids and tannin contents to evaluate the anti-diabetic activities of Salacia oblonga stem followed by GC-MS analysis. It explores the natural compounds and the potential α-amylase and α-glucosidase inhibitory actions of stem extracts. The aqueous stem extract was selected from other extracts (ethanol, acetone, petroleum ether and chloroform) for the in vitro study of anti-diabetic activity by alpha amylase and alpha glucosidase inhibitory assays. The stem extract was also analyzed by gas chromatography mass spectrometry to identify the natural chemical components. Phytochemical analysis of aqueous stem extract showed major classes of secondary metabolites such as phenols, flavonoids, alkaloids, terpenoids, tannins, saponins. The total flavonoid, terpenoid, and tannin contents were quantified as 19.82±0.06 mg QE/g, 96.2±0.20 mg/g and 11.25±0.03 mg TAE/g respectively. The percentage inhibition of assays showed maximum inhibitory effects (59.46±0.04% and 68.51±0.01%) at a concentration of 100 mg/mL. The IC50 values of stem extract was found to be 73.56 mg/mL and 80.90 mg/mL for alpha amylase and alpha glucosidase inhibition. Fifteen chemical constituents were found by GC-MS analysis. This study suggest the aqueous stem extract of Salacia oblonga might be considered as potential source of bio active constituents with excellent antidiabetic activity.
DOI: 10.6084/m9.figshare.14290785.v1
title: Effects of Arctium lappa aqueous extract on lipid profile and hepatic enzyme levels of sucrose-induced metabolic syndrome in female rats

description: ABSTRACT Arctium lappa is known to have antioxidant and antidiabetic effects in traditional medicine. Objectives: The aim of this paper was to study the effects of A. lappa root extract (AE) on lipid profile and hepatic enzyme levels in sucrose-induced metabolic syndrome (MS) in female rats. The study used 40 adult female Wistar rats weighing 150 g-250 g randomly divided into five groups: control, metabolic syndrome (MS), metabolic syndrome+AE at 50,100, 200 mg/kg. MS was induced by administering 50% sucrose in drinking water for 6 weeks. AE was intra-peritoneally administered daily at doses of 50,100, and 200 mg/kg for two sequential weeks at the end of the fourth week in metabolic syndrome rats. Twenty-four hours after the last administration of AE, blood was collected and centrifuged, and then the serum was used for the measurement of lipid profile and hepatic enzyme. Serum glucose, insulin, fasting insulin resistance index, body weight, water intake, lipid profile, and hepatic enzymes were significantly increased although food intake was decreased in MS rats compared to the control rats. The lipids and liver enzymes were reduced by AE extracts in the MS group. This study showed that the A. lappa root aqueous extract exhibits a hypolipidemic activity of hyperlipidemic rats. This activity is practically that of a triple-impact antioxidant, hypolipidemic, and hepatoprotective.
DOI: 10.6084/m9.figshare.6272804.v1
title: Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity

description: ABSTRACT Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional (Middlebrook 7H9, Ogawa Kudoh-OK) and in nitrate-supplemented media (Middlebrook 7H9-N, Ogawa Kudoh-N). An aliquot from the media directly reacted with Griess reagent (7H9-N and OK-N) every five days, or transferred to a nitrate substrate solution (7H9, OK). Nitrate to nitrite reduction was considered positive, revealed by the pink color, indicating bacterial growth. As reference method, the Mycobacteria Growth Indicator Tube (MGIT) was used for sensitivity calculations and statistical analysis. 7H9-N and OK-N assays proved to perform better in detecting M. tuberculosis than conventional assays (7H9 and OK). Indeed, broth nitrate-supplemented medium (7H9-N) was comparable to MGIT to detect M. tuberculosis, except in growth detection time. Results show that 7H9-N may be used as an alternative tool particularly in low-income countries since it is a simple and cheap technique, and does not restrict diagnosis to single-source products.
DOI: 10.6084/m9.figshare.14307137.v1
title: Nitrogen fertilization management in white oat using spectral indices

description: ABSTRACT Remote sensing techniques have been considered a new technology in worldwide agriculture for diagnosing the plant nutritional demand. Fertilizer management efficiency is a goal to be achieved, and modern tools based on remote sensing are promising for monitoring the crop needs. This study aimed to evaluate the agronomic performance and relative economic return of white oat under nitrogen rates, as well as to verify whether the normalized difference vegetation index (NDVI) and leaf chlorophyll index (LCI) could be used for topdressing nitrogen fertilization management, in white oat. Treatments consisted of five topdressing nitrogen fertilization strategies: T1 - 160 kg ha-1 (reference rate); T2 - 90 kg ha-1 (recommended rate); T3 - 60 kg ha-1 (economic rate); T4 - 30 kg ha-1 (when NDVI &lt; 90 % of T1); and T5 - 30 kg ha-1 (when LCI &lt; 90 % of T1). The white oat did not respond to the topdressing nitrogen fertilization. Its temporal monitoring using spectral indices allowed dispensing the topdressing nitrogen fertilization without reducing the grain and biomass yields and the leaf nitrogen content, when compared to the recommended management (90 kg ha-1 of N as topdressing), with no differences between the evaluated spectral indices. Thus, both the NDVI and LCI spectral indices are promising tools for the topdressing nitrogen fertilization management in the white oat crop.
DOI: 10.6084/m9.figshare.14290811.v1
title: Identification of neprilysin as a potential target of arteannuin using computational drug repositioning

description: ABSTRACT The discovery of arteannuin (qinghaosu) in the 20th Century was a major advance for medicine. Besides functioning as a malaria therapy, arteannuin is a pharmacological agent in a range of other diseases, but its mechanism of action remains obscure. In this study, the reverse docking server PharmMapper was used to identify potential targets of arteannuin. The results were checked using the chemical-protein interactome servers DRAR-CPI and DDI-CPI, and verified by AutoDock Vina. The results showed that neprilysin (also known as CD10), a common acute lymphoblastic leukaemia antigen, was the top disease-related target of arteannuin. The chemical-protein interactome and docking results agreed with those of PharmMapper, further implicating neprilysin as a potential target. Although experimental verification is required, this study provides guidance for future pharmacological investigations into novel clinical applications for arteannuin.
DOI: 10.6084/m9.figshare.6504287
title: Augmented cytotoxic, mutagenic and genotoxic response triggered by carvedilol and celecoxib combinations

description: Abstract It is understood that drugs regardless of their order of administration can exhibit drug interactions. Established on the fact that treatment of hypertension may last for decades and prolong usage of multiple drug regimen may induce substantial pathophysiological changes. Hence, This study was designed to evaluate the possible synergistic toxic effects of anti-hypertensive (carvedilol), and anti-inflammatory drug (celecoxib) alone and in combinations. Well-established MTT assay, Single Cell Gel Electrophoresis (SCGE) and Ames assay were employed to evaluate the toxicity at cellular level. Results from MTT assay on Vero cell line revealed that drug combinations have more pronounced anti-proliferative activity with combine IC50 value of 13.7:47.8 µg/mL. Likewise, exposure of peripheral blood mononuclear cells with drug combinations revealed significant (P&lt;0.05) DNA damage (Class 3) in a dose dependent manner at concentrations ≥ 0.78: 2.34 µg/mL. However, carvedilol and celecoxib were non mutagenic against either mutant strain (TA 100 and TA 98) and combinations have also shown mild to moderate mutagenic potential. Nevertheless, upon addition of metabolic activation enzyme, concentration &lt;12.5:37.5 µg/plate exhibited significant (P&lt;0.05) mutagenicity against both tester strains. In conclusion, this study provides additional genotoxicity and mutagenicity data that could be used in considering options for formulating regimens with reduced mutagenic potential.