cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5126110.v1
title: Supplementary Material for: Pre-End-Stage Renal Disease Care Not Associated with Dialysis Facility Neighborhood Poverty in the United States

description: <b><i>Background:</i></b> Receipt of nephrology care prior to end-stage renal disease (ESRD) is a strong predictor of decreased mortality and morbidity, and neighborhood poverty may influence access to care. Our objective was to examine whether neighborhood poverty is associated with lack of pre-ESRD care at dialysis facilities. <b><i>Methods:</i></b> In a multi-level ecological study using geospatially linked 2007-2010 Dialysis Facility Report and 2006-2010 American Community Survey data, we examined whether<b> </b>high<b> </b>neighborhood poverty (≥20% of households in census tract living below poverty) was associated with dialysis facility-level lack of pre-ESRD care (percentage of patients with no nephrology care prior to dialysis start) in mixed-effects models, adjusting for facility and neighborhood confounders and allowing for neighborhood and regional random effects. <b><i>Results:</i></b> Among the 5,184 facilities examined, 1,778 (34.3%) were located in a high-poverty area. Lack of pre-ESRD care was similar in poverty areas (30.8%) and other neighborhoods (29.6%). With adjustment, the absolute increase in percentage of patients at a facility with no pre-ESRD care associated with facility location in a poverty area versus other neighborhood was only 0.08% (95% CI -1.32, 1.47; p = 0.9). Potential effect modification by race and income inequality was detected. <b><i>Conclusion:</i></b> Despite previously reported detrimental effects of neighborhood poverty on health, facility neighborhood poverty was not associated with receipt of pre-ESRD care, suggesting no need to target interventions to increase access to pre-ESRD care at facilities in poorer geographic areas.
DOI: 10.6084/m9.figshare.5126098
title: Supplementary Material for: Self-Reported Skin Morbidities and Health-Related Quality of Life: A Population-Based Nested Case-Control Study

description: <b><i>Background:</i></b> Impaired dermatological health-related quality of life (HRQoL) has been observed in hospital-based studies, but little is known on a population-based level. <b><i>Objective:</i></b> To investigate self-reported dermatological HRQoL in the general population. <b><i>Methods:</i></b> Hidradenitis suppurativa, psoriasis, pimples, hand rash or atopic eczema were identified using questionnaires in a 15,177 person population sample. A nested case-control study of 180 cases and 259 controls was made using the Dermatology Life Quality Index (DLQI), Skindex-29 and EQ-5D. <b><i>Results:</i></b> Cases had higher scores in DLQI and Skindex-29 and a lower score in EQ-5D, suggesting lower HRQoL. Adjusting for age and sex, the differences in Skindex-29 and DLQI were significant (p &lt; 0.001). <b><i>Conclusions:</i></b> Persons with self-reported skin morbidity had lower HRQoL than the general population. The impairment is not as significant as in studies of hospital-based cases, but considering the high prevalence of skin diseases it may still represent a significant burden of disease on society in aggregate.
DOI: 10.6084/m9.figshare.5126101.v1
title: Supplementary Material for: Functional Closure of the Ductus Arteriosus at Birth: Evidence against an Intermediary Role of Angiotensin II

description: The fetal ductus arteriosus (DA) closes postnatally first functionally and then structurally. Normal rise in blood oxygenation is regarded as a prime trigger, but closure may occur more slowly without this stimulus. Here, our aim was<b> </b>to assess the role of angiotensin II (Ang II) in functional closure of DA since its action may not be conditioned by oxygen. Experiments were performed with wild-type fetal and neonatal mice, using whole-body freezing technique to assess DA caliber in vivo. Transcripts for Ang II type 1 (AT<sub>1</sub>R) and type 2 (AT<sub>2</sub>R) receptors were also examined. We found that<b> </b>the AT<sub>1</sub>R antagonist olmesartan had no effect in the fetus, but delayed ductus closure in the neonate. However, this response was short-lived and disappeared upon concomitant treatment with the AT<sub>2</sub>R antagonist PD123319. Coincidentally, olmesartan promoted the <i>Agtr2 </i>transcript. We conclude that AT<sub>1</sub>R-based Ang II has no role in the functional closure of DA. Conversely, the compound may modulate this process through AT<sub>2</sub>R-mediated vasodilatation.
DOI: 10.6084/m9.figshare.5126107.v1
title: Supplementary Material for: Low Expression of Transforming Growth Factor Beta-1 in Cancer Tissue Predicts a Poor Prognosis for Patients with Stage III Rectal Cancers

description: <b><i>Objective:</i></b> Transforming growth factor beta (TGF-β) plays an important role in tumorigenesis and metastasis. It works as a tumor suppressor in the normal colon, but acts as a cancer promoter during the late stages of colorectal carcinogenesis. High expression of TGF-β is known to be associated with advanced stages, tumor recurrence and decreased survival of patients. We investigated the expression of TGF-β and its signaling axis molecules and evaluated their prognostic significance in patients with stage III rectal cancers. <b><i>Methods:</i></b> Tissues from 201 cases of stage III rectal cancer were subjected to immunohistochemistry for TGF-β1, type II TGF-β receptor, Smad3, Smad4 and Smad7 proteins. The immunoactivities of these molecules were evaluated and the results were compared with clinicopathological variables including patient survival. <b><i>Results:</i></b> Low expression of TGF-β1 protein was correlated with a decreased disease-free survival in univariate Kaplan-Meier (p = 0.003) and multivariate Cox regression (HR 9.188 and 95% CI 1.256-67.198, p = 0.029) analyses. The loss of Smad4 protein expression was associated with a reduction in disease-free survival in the univariate analysis, but this finding was not significant after the multivariate analysis. <b><i>Conclusion:</i></b> Low expression of TGF-β1 protein is associated with a poor prognosis for patients with stage III rectal cancers.
DOI: 10.6084/m9.figshare.5126092
title: Supplementary Material for: ABCA1 rs1883025 Polymorphism Shows No Association with Neovascular Age-Related Macular Degeneration or Polypoidal Choroidal Vasculopathy in a Northern Chinese Population

description: <b><i>Purpose:</i></b> To analyze the association between ABCA1 rs1883025 variants with neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV) in a northern Chinese population. <b><i>Methods:</i></b> The study enrolled 900 subjects, including 300 controls, 300 cases with nAMD and 300 cases with PCV. Genomic DNA was extracted from venous blood leukocytes. Single-nucleotide polymorphisms in the ABCA1 (rs1883025) gene were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. <b><i>Results:</i></b> The ABCA1 rs1883025 polymorphism was not significantly associated with nAMD (22.5%; p &gt; 0.05) or PCV (20.8%; p &gt; 0.05) in a northern Chinese population. The association remained insignificant after adjustment for age and gender differences (p &gt; 0.05). <b><i>Conclusions:</i></b> This study suggests that ABCA1 rs1883025 variants are not associated with nAMD or PCV in a Chinese population, which is likely due to an ethnic difference.
DOI: 10.6084/m9.figshare.5126098.v1
title: Supplementary Material for: Self-Reported Skin Morbidities and Health-Related Quality of Life: A Population-Based Nested Case-Control Study

description: <b><i>Background:</i></b> Impaired dermatological health-related quality of life (HRQoL) has been observed in hospital-based studies, but little is known on a population-based level. <b><i>Objective:</i></b> To investigate self-reported dermatological HRQoL in the general population. <b><i>Methods:</i></b> Hidradenitis suppurativa, psoriasis, pimples, hand rash or atopic eczema were identified using questionnaires in a 15,177 person population sample. A nested case-control study of 180 cases and 259 controls was made using the Dermatology Life Quality Index (DLQI), Skindex-29 and EQ-5D. <b><i>Results:</i></b> Cases had higher scores in DLQI and Skindex-29 and a lower score in EQ-5D, suggesting lower HRQoL. Adjusting for age and sex, the differences in Skindex-29 and DLQI were significant (p &lt; 0.001). <b><i>Conclusions:</i></b> Persons with self-reported skin morbidity had lower HRQoL than the general population. The impairment is not as significant as in studies of hospital-based cases, but considering the high prevalence of skin diseases it may still represent a significant burden of disease on society in aggregate.
DOI: 10.6084/m9.figshare.5126083
title: Supplementary Material for: Comparison of Body Surface Area versus Weight-Based Growth Hormone Dosing for Girls with Turner Syndrome

description: <b><i>Background/Aims:</i></b> Growth Hormone (GH) dosage in childhood is adjusted for body size, but there is no consensus whether body weight (BW) or body surface area (BSA) should be used. We aimed at comparing the biological effect and cost-effectiveness of GH treatment dosed per m<sup>2</sup> BSA in comparison with dosing per kg BW in girls with Turner syndrome (TS). <b><i>Methods:</i></b> Serum IGF-I, GH dose, and adult height gain (AHG) from girls participating in two Dutch and five Swedish studies on the efficacy of GH were analyzed, and the cumulative GH dose and costs were calculated for both dose adjustment methods. Additional medication included estrogens (if no spontaneous puberty occurred) and oxandrolone in some studies. <b><i>Results:</i></b> At each GH dose, the serum IGF-I standard deviation score remained stable over time after an initial increase after the start of treatment. On a high dose (at 1 m<sup>2</sup> equivalent to 0.056-0.067 mg/kg/day), AHG was at least equal on GH dosed per m<sup>2</sup> BSA compared with dosing per kg BW. The cumulative dose and cost were significantly lower if the GH dose was adjusted for m<sup>2</sup> BSA. <b><i>Conclusion:</i></b> Dosing GH per m<sup>2</sup> BSA is at least as efficacious as dosing per kg BW, and is more cost-effective.
DOI: 10.6084/m9.figshare.5126086
title: Supplementary Material for: Changes in the Bacterial Community Structure in Stored Wormbed Leachate

description: Organic wastes, such as cow manure, are often composted with earthworms (vermicomposting) while excess water is drained and collected. This wormbed leachate is nutrient-rich and it has been extensively used to fertilize plants. However, it is derived partially from a not yet finished compost process and could exhibit phytotoxicity or contain potentially hazardous microorganisms. The bacterial community in wormbed leachate derived from vermicomposting of cow manure was studied by pyrosequencing the 16S rRNA gene. The fresh wormbed leachate was rich in Mollicutes, particularly the genus <i>Acholeplasma</i> which contain phytopathogen species. The abundance of the Mollicutes decreased when the leachate was stored, while that of the Rhizobiales and the genus <i>Pseudomonas</i> increased. The bacterial communities changed rapidly in the leachate during storage. The changes in ammonium, nitrate and inorganic carbon content of the wormbed leachate when stored were correlated to changes in the bacterial community structure. It was found that storage of the wormbed leachate might be required before it can be applied to crops as large proportions of potentially plant pathogens were found in the fresh leachate.
DOI: 10.6084/m9.figshare.5126089
title: Supplementary Material for: Prevalence and Determinants of Parathyroid Dysfunction in Elderly Patients on Hemodialysis

description: <b><i>Background:</i></b> The goal of this study was to investigate underlying factors of parathyroid dysfunction in elderly patients undergoing maintenance hemodialysis. <b><i>Methods:</i></b> A total of 286 patients on maintenance hemodialysis were included. Hemoglobin, serum creatinine (Scr), blood urea nitrogen (BUN), serum calcium, serum phosphorus (P), intact parathyroid hormone (iPTH), and serum albumin (Alb) were measured and analyzed both before and after dialysis. <b><i>Results:</i></b> A higher incidence of low iPTH level (&lt;150 pg/l) was observed in the elderly group than that in the non-elderly group (55.8 vs. 36.7%, p &lt; 0.05). Elderly patients had a shorter dialysis duration, lighter dry weight, lower concentrations of BUN, Scr, P, iPTH, Alb and standard protein nitrogen present rate (nPNA) compared to that of non-elderly group patients (p &lt; 0.05). <b><i>Conclusions:</i></b> Low iPTH level occurs more frequently in elderly hemodialysis patients. Furthermore, age, serum P, serum Alb and nPNA were independently associated with a low iPTH level.
DOI: 10.6084/m9.figshare.5126080
title: Supplementary Material for: Immunoreactivity Distribution of Vasotocin and Vasoactive Intestinal Peptide in Brain Nuclei of Two Songbird Species with Different Breeding Systems

description: Vasopressin influences social behaviour in mammals, in particular social recognition and bonding. However, much less is known about its avian analogue, vasotocin, although vasotocin appears to modulate singing behaviour and agonistic interactions together with vasoactive intestinal peptide (VIP) in some songbirds. The objectives of our study were to compare the expression of vasotocin and VIP in brain nuclei hypothetised to be part of the social behavioural network, i.e. septal areas, bed nucleus of the stria terminalis and medial preoptic nucleus (POM), in two songbird species in the wild: the blue tit <i>(Cyanistes caeruleus)</i> and European penduline tit <i>(Remiz pendulinus)</i>. These two closely related passerine birds differ in their pair bonding and mating systems: blue tits are socially monogamous with extensive pair bond lasting for several months, whereas in the European penduline tit, pair bond is short and it dissolves during or after laying of the eggs. The two species did not differ in the distribution of vasotocin in the observed brain regions; however, VIP was more abundant in all three regions of penduline tits than in blue tits. We found a sex difference in favour of males in the distribution of vasotocin- and VIP-immunoreactive neurones, fibres and terminals in all three regions in penduline tits. In blue tits, such gender differences were only observed in the POM. The limited differences between the two species suggest that the levels of vasotocin and VIP in the socially relevant brain regions are likely influenced by many other social or environmental factors than just by differences in the duration of pair bonding.
DOI: 10.6084/m9.figshare.5126092.v1
title: Supplementary Material for: ABCA1 rs1883025 Polymorphism Shows No Association with Neovascular Age-Related Macular Degeneration or Polypoidal Choroidal Vasculopathy in a Northern Chinese Population

description: <b><i>Purpose:</i></b> To analyze the association between ABCA1 rs1883025 variants with neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV) in a northern Chinese population. <b><i>Methods:</i></b> The study enrolled 900 subjects, including 300 controls, 300 cases with nAMD and 300 cases with PCV. Genomic DNA was extracted from venous blood leukocytes. Single-nucleotide polymorphisms in the ABCA1 (rs1883025) gene were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. <b><i>Results:</i></b> The ABCA1 rs1883025 polymorphism was not significantly associated with nAMD (22.5%; p &gt; 0.05) or PCV (20.8%; p &gt; 0.05) in a northern Chinese population. The association remained insignificant after adjustment for age and gender differences (p &gt; 0.05). <b><i>Conclusions:</i></b> This study suggests that ABCA1 rs1883025 variants are not associated with nAMD or PCV in a Chinese population, which is likely due to an ethnic difference.
DOI: 10.6084/m9.figshare.5126077
title: Supplementary Material for: Visual Acuity in Mammals: Effects of Eye Size and Ecology

description: Previous comparative research has attributed interspecific variation in eye size among mammals to selection related to visual acuity. Mammalian species have also been hypothesized to differ in visual acuity partly as a result of differences in ecology. While a number of prior studies have explored ecological and phylogenetic effects on eye shape, a broad comparative analysis of the relationships between visual acuity, eye size and ecology in mammals is currently lacking. Here we use phylogenetic comparative methods to explore these relationships in a taxonomically and ecologically diverse sample of 91 mammal species. These data confirm that axial eye length and visual acuity are significantly positively correlated in mammals. This relationship conforms to expectations based on theoretical optics and prior analyses of smaller comparative samples. Our data also demonstrate that higher visual acuity in mammals is associated with: (1) diurnality and (2) predatory habits once the effects of eye size and phylogeny have been statistically controlled. These results suggest that interspecific variation in mammalian visual acuity is the result of a complex interplay between phylogenetic history, visual anatomy and ecology.
DOI: 10.6084/m9.figshare.5126083.v1
title: Supplementary Material for: Comparison of Body Surface Area versus Weight-Based Growth Hormone Dosing for Girls with Turner Syndrome

description: <b><i>Background/Aims:</i></b> Growth Hormone (GH) dosage in childhood is adjusted for body size, but there is no consensus whether body weight (BW) or body surface area (BSA) should be used. We aimed at comparing the biological effect and cost-effectiveness of GH treatment dosed per m<sup>2</sup> BSA in comparison with dosing per kg BW in girls with Turner syndrome (TS). <b><i>Methods:</i></b> Serum IGF-I, GH dose, and adult height gain (AHG) from girls participating in two Dutch and five Swedish studies on the efficacy of GH were analyzed, and the cumulative GH dose and costs were calculated for both dose adjustment methods. Additional medication included estrogens (if no spontaneous puberty occurred) and oxandrolone in some studies. <b><i>Results:</i></b> At each GH dose, the serum IGF-I standard deviation score remained stable over time after an initial increase after the start of treatment. On a high dose (at 1 m<sup>2</sup> equivalent to 0.056-0.067 mg/kg/day), AHG was at least equal on GH dosed per m<sup>2</sup> BSA compared with dosing per kg BW. The cumulative dose and cost were significantly lower if the GH dose was adjusted for m<sup>2</sup> BSA. <b><i>Conclusion:</i></b> Dosing GH per m<sup>2</sup> BSA is at least as efficacious as dosing per kg BW, and is more cost-effective.
DOI: 10.6084/m9.figshare.5126071
title: Supplementary Material for: Identification of Novel Transmembrane Proteins in Scirrhous-Type Gastric Cancer by the <b><i>Escherichia coli</i></b> Ampicillin Secretion Trap (CAST) Method: <b><i>TM9SF3</i></b> Participates in Tumor Invasion and Serves as a Prognostic Factor

description: <b><i>Objective:</i></b> Scirrhous-type gastric cancer (GC) is highly aggressive and has a poor prognosis due to rapid cancer cell infiltration accompanied by extensive stromal fibrosis. The aim of this study is to identify genes that encode transmembrane proteins frequently expressed in scirrhous-type GC. <b><i>Methods:</i></b> We compared <i>Escherichia coli</i> ampicillin secretion trap (CAST) libraries from 2 human scirrhous-type GC tissues with a normal stomach CAST library. By sequencing 2,880 colonies from scirrhous CAST libraries, we identified a list of candidate genes. <b><i>Results:</i></b> We focused on the TM9SF3 gene because it has the highest clone count, and immunohistochemical analysis demonstrated that 46 (50%) of 91 GC cases were positive for TM9SF3, which was observed frequently in scirrhous-type GC. TM9SF3 expression showed a significant correlation with the depth of invasion, tumor stage and undifferentiated GC. There was a strong correlation between TM9SF3 expression and poor patient outcome, which was validated in two separate cohorts by immunostaining and quantitative RT-PCR, respectively. Transient knockdown of the TM9SF3 gene by siRNA showed decreased tumor cell-invasive capacity. <b><i>Conclusion:</i></b> Our results indicate that TM9SF3 might be a potential diagnostic and therapeutic target for scirrhous-type GC.
DOI: 10.6084/m9.figshare.5126074
title: Supplementary Material for: Creativity and Psychopathology: A Systematic Review

description: <b><i>Background:</i></b> The possible link between creativity and psychopathology has been a long-time focus of research up to the present day. However, the research results in this field are heterogeneous and contradictory. Links between creativity and specific psychiatric disorders have been confirmed and refuted in different studies. This disparity is partly explained by the methodological challenges peculiar to this field. <b><i>Methods:</i></b> In this systematic review of the literature from 1950, research articles in the field of creativity and psychopathology are presented, focusing on the methodology and results of the collected studies. <b><i>Results:</i></b> This review confirms the methodological problems and the heterogeneity of the study designs and results. The assessment of psychopathology, but more so of creativity, remains a fundamental challenge. On the whole, study results cautiously confirm an association between creativity and both bipolar disorder and schizotypy. <b><i>Conclusion:</i></b> The research on creativity and psychopathology is hampered by serious methodological problems. Study results are to be interpreted with caution and future research needs more methodological rigor.
DOI: 10.6084/m9.figshare.5126068
title: Supplementary Material for: Proteomic Analysis of Whole Glomeruli in Patients with IgA Nephropathy Using Microsieving

description: <b><i>Background:</i></b> To promote understanding of immunoglobulin A nephropathy (IgAN) pathophysiology, we tried to elucidate glomerular protein profiles in IgAN, using microsieving that we established recently to isolate glomeruli from renal biopsy samples and proteomic approaches. <b><i>Methods:</i></b> Glomeruli were isolated from renal biopsy samples of patients with IgAN (n = 5) and with minimal change nephrotic syndrome (MCNS; n = 5) using microsieving. Proteins extracted from the isolated glomeruli were separated by 2-dimensional differential gel electrophoresis (2D-DIGE). Proteins with different amounts between the two groups were identified by mass spectrometry. One of the identified proteins, α-actinin-4 (ACTN4), was further analyzed by Western blotting, RT-polymerase chain reaction (PCR), and immunohistochemistry. <b><i>Results:</i></b> By 2D-DIGE, 72 out of the detected 1,170 protein spots showed significantly different intensity between the two groups (p &lt; 0.05). Thirty-four out of the 72 protein spots showed more than 1.5-fold or less than 1/1.5-fold intensity, out of which 16 protein spots were successfully identified. No microbial protein was identified. ACTN4 molecules with a low molecular weight of approximately 77 kDa were found to increase in the IgAN group. Lack of an N-terminal part of ACTN4 was demonstrated by Western blotting. No defect of mRNA for ACTN4 was evidenced by RT-PCR. Predominant existence of ACTN4 in capillary walls of glomeruli of IgAN patients was demonstrated by immunohistochemistry in glomerular sections of patients with IgAN. <b><i>Conclusion:</i></b> Use of microsieving enabled us to biochemically analyze glomerular proteins in renal biopsy samples from patients with glomerular diseases. With this method, we demonstrated skewed glomerular protein profiles in IgAN.