DOI: 10.5061/dryad.wwpzgmswd
title: Adolescent alcohol exposure, pain, and synaptic function at BLA inputs onto prelimbic neurons

description: This dataset covers mechanical and thermal sensitivity in PV-Cre rats
 (RRID:RRRC_00773) beginning in adolescence and continuing into adulthood.
 The rats were tested weekly for eight weeks. After the first test, half
 the rats began adolescent intermittent ethanol vapor exposure which
 continued for 4 weeks. Rats then underwent surgery to express
 channelrhodopsin in the BLA, and a viral cre-depedent mCherry tag in the
 prelimbic cortex. Retrobeads were injected in the ventrolateral
 periaqueductal gray. This allowed for slice electrophysiology recordings
 from prelimbic parvalbumin interneurons and prelimbic pyramidal neurons
 projecting to the ventrolateral periaqueductal gray. After recovering from
 the surgery, rats underwent a carrageenan inflammatory paw pain challenge.
 After assessing the effects of adolescent alcohol exposure in conjuction
 with the pain challenge on mechanical and thermal sensitivity, slice
 electrophysiology recordings were obtained from neurons in the prelimbic
 cortex. Parameters of interest for these recordings included
 excitatory/inhibitory balance, AMPA/NMDA ratio, and aEPSCs at BLA inputs
 onto PrL parvalbumin interneurons and periaqueductal gray projecting
 pyramidal neurons. Measures of intrinsic excitability were also obtained.
description: # Adolescent alcohol exposure, pain, and synaptic function at BLA inputs
 onto prelimbic neurons Dataset DOI:
 [10.5061/dryad.wwpzgmswd](10.5061/dryad.wwpzgmswd) ## Description of the
 data and file structure These data include both behavioral assessments of
 mechanical and thermal pain sensitivity as well as data from slice
 electrophysiology recordings measuring synaptic function at basolateral
 amygdala inputs onto prelimbic parvalbumin positive interneurons and
 pyramidal neurons projecting from the prelimbic cortex to the
 ventrolateral periaqueductal gray. For the behavioral assessments, the
 electronic Von Frey and Hargreaves tests were used. Data were collected
 weekly for 8 weeks beginning at postnatal day 24 (PD 24) and continuing
 until PD 80. Half the rats began alcohol vapor exposure at PD 28. Rats
 underwent final reflexive pain assessments at approximately PD 109. This
 involved a baseline pain assessment followed by a carrageenan or saline
 (control) injection into the hindpaw. The effects of carrageenan on
 mechanical and thermal sensitivity were then measured at 2, 6, and 24
 hours postinjection. Brain slices containing the prelimbic cortex were
 then prepared and electrophysiological recordings performed. Data from
 these recordings includes measures of intrinsic excitability,
 excitatory/inhibitory balance, AMPA/NMDA ratio, and asynchronous EPSCs.
 ### Files and variables #### File:
 Longitudinal\_mechanical\_and\_thermal\_thresholds.csv
 **Description:** Longitudinal data on mechanical and thermal sensitivity
 in PV-Cre rats. ##### Variables * Sex: Sex of the rat. * Tx Group: Air
 (control) or AIE (adolescent intermittent ethanol) * Age (Postnatal Day):
 Age of the rat at the time of the data collection. * Withdrawal Threshold
 (gram-force): Amount of force required to elicit a paw withdrawal
 response. Reported in gram-force as measured using an electronic von Frey
 apparatus. * Hargreaves Withdrawal Latency (s): Amount of time (in
 seconds) that elapsed before the hindpaw was withdraw during the
 Hargreaves test. #### File: Membrane\_properties.csv
 **Description:** Membrane properties of prelimbic neurons. ##### Variables
 * Sex: Sex of the rat. * Challenge Group: Saline (control) or carrageenan
 (inflammatory paw pain). * Tx Group: Air (control) or AIE (adolescent
 intermittent ethanol) * Neuron Type: PRL-PAG (Prelimbic pyramidal neuron
 projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic
 parvalbumin positive interneuron). * rm (MOhm): Membrane resistance in
 megaohms. * vm (mV): Resting Membrane Potential in millivolts. #### File:
 PD109\_Baseline\_mechanical\_and\_thermal.csv **Description:** Baseline
 mechanical and thermal sensitivity prior to the carrageenan paw pain
 challenge. The test occurred at approximately PD109. ##### Variables *
 Sex: Sex of the rat. * Tx Group: Air (control) or AIE (adolescent
 intermittent ethanol) * Withdrawal Threshold (gram-force): Amount of force
 required to elicit a paw withdrawal response. Reported in gram-force as
 measured using an electronic von Frey apparatus. * Hargreaves Withdrawal
 Latency (s): Amount of time (in seconds) that elapsed before the hindpaw
 was withdraw during the Hargreaves test. #### File: Sag\_Ratio.csv
 **Description:** Sag ratio of prelimbic neurons. ##### Variables *
 Sex: Sex of the rat. * Challenge Group: Saline (control) or carrageenan
 (inflammatory paw pain). * Tx Group: Air (control) or AIE (adolescent
 intermittent ethanol) * Neuron Type: PRL-PAG (Prelimbic pyramidal neuron
 projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic
 parvalbumin positive interneuron). * sag ratio (%): Neuronal sag ratio.
 #### File: PD31-80\_average\_mechanical\_and\_thermal\_threshold.csv
 **Description:** Averaged mechanical and thermal threshold for rats during
 and after adolescent alcohol vapor exposure. ##### Variables * Sex: Sex of
 the rat. * Tx Group: Air (control) or AIE (adolescent intermittent
 ethanol) * Withdrawal Threshold (gram-force): Average amount of force
 required to elicit a paw withdrawal response. Reported in gram-force as
 measured using an electronic von Frey apparatus. The average was taken
 across values beginning at PD31 and continuing until PD80.
 See Longitudinal_mechanical_and_thermal_thresholds.csv for unaveraged
 values. * Hargreaves Withdrawal Latency (s): Average amount of time (in
 seconds) that elapsed before the hindpaw was withdraw during the
 Hargreaves test. The average was taken across values beginning at PD31 and
 continuing until PD80.
 See Longitudinal_mechanical_and_thermal_thresholds.csv for unaveraged
 values. #### File: Rheobase.csv **Description:** Rheobase of prelimbic
 pyramidal neurons projecting to the ventrolateral periaqueductal gray.
 ##### Variables * Sex: Sex of the rat. * Challenge Group: Saline (control)
 or carrageenan (inflammatory paw pain). * Tx Group: Air (control) or AIE
 (adolescent intermittent ethanol) * Neuron Type: PRL-PAG (Prelimbic
 pyramidal neuron projecting to the ventrolateral periaqueductal gray) or
 PVIN (prelimbic parvalbumin positive interneuron). * rheobase (pA):
 Neuronal rheobase.  #### File: AMPA-NMDA\_Ratio.csv
 **Description:** Monosynaptic optically evoked AMPA and NMDA currents at
 basolateral amygdala inputs onto prelimbic neurons. ##### Variables * Sex:
 Sex of the rat. * Challenge Group: Saline (control) or carrageenan
 (inflammatory paw pain). * Tx Group: Air (control) or AIE (adolescent
 intermittent ethanol) * Neuron Type: PRL-PAG (Prelimbic pyramidal neuron
 projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic
 parvalbumin positive interneuron). * oAMPA_+40 (pA): Peak amplitude of the
 optically evoked AMPA current measured while holding the cell at +40 mV. *
 oAMPA_-70 (pA): Peak amplitude of the optically evoked AMPA current
 measured while holding the cell at -70 mV. * oNMDA (pA): Peak amplitude of
 the optically evoked NMDA current measured while holding the cell at +40
 mV. * AMPA_+40/NMDA ratio: Ratio of amplitude of the oAMPA (+40 mV)
 current to the NMDA current. * AMPA_-70/NMDA ratio: Ratio of the amplitude
 of the oAMPA (-70 mV) current to the NMDA current. * rectification index:
 AMPA rectification index computed as the ratio of the oAMPA (+40 mV) /
 oAMPA (-70 mV) peak amplitudes. #### File: AP\_Threshold\_\_\_AHP.csv
 **Description:** Action potential threshold and afterhyperpolarization of
 neurons in the prelimbic cortex. ##### Variables * Sex: Sex of the rat. *
 Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
 * Tx Group: Air (control) or AIE (adolescent intermittent ethanol) *
 Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the
 ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive
 interneuron). * AP threshold (mV): Action potential threshold measured in
 millivolts. * afterhyperpolarization (mV): Neuronal afterhyperpolarization
 measured in millivolts. #### File:
 Carrageenan\_average\_mechanical\_and\_thermal.csv
 **Description:** Average mechanical and thermal sensitivity of rats
 following a saline or carrageenan injection in the right hindpaw. #####
 Variables * Sex: Sex of the rat. * Challenge Group: Saline (control) or
 carrageenan (inflammatory paw pain). * Tx Group: Air (control) or AIE
 (adolescent intermittent ethanol) * Withdrawal Threshold (% Baseline):
 Average withdrawal threshold expressed as a percentage of each rats
 baseline withdrawal force. The average was taken across the 2-, 6-, and
 24-hr postinjection timepoints. 
 See PD109_Baseline_mechanical_and_thermal.csv for baseline values.
 See Carrageenan_longitudinal_mechanical_and_thermal.csv for the values
 that were averaged. * Hargreaves Withdrawal Latency (% Baseline): Average
 amount of time that elapsed before the hindpaw was withdraw during the
 Hargreaves test. The withdrawal latency is expressed as a percentage of
 the baseline withdrawal latency. The average was taken across the 2-, 6-,
 and 24-hr postinjection timepoints.
 See PD109_Baseline_mechanical_and_thermal.csv for baseline
 values. See Carrageenan_longitudinal_mechanical_and_thermal.csv for the
 values that were averaged. #### File:
 Carrageenan\_longitudinal\_mechanical\_and\_thermal.csv
 **Description:** Measurements of mechanical and thermal sensitivity taken
 at 2-, 6-, and 24-hr following an injection with saline or carrageenan in
 the right hindpaw. ##### Variables * Sex: Sex of the rat. * Challenge
 Group: Saline (control) or carrageenan (inflammatory paw pain). * Tx
 Group: Air (control) or AIE (adolescent intermittent ethanol) * Time (hr):
 Time postinjection. * Withdrawal Threshold (% Baseline): The withdrawal
 threshold expressed as a percentage of the baseline
 value. See PD109_Baseline_mechanical_and_thermal.csv for baseline values.
 The withdrawal threshold was obtained using an electronic von Frey
 apparatus. * Hargreaves Withdrawal Latency (% Baseline): The withdrawal
 latency expressed as a percentage of the baseline
 value. See PD109_Baseline_mechanical_and_thermal.csv for baseline values.
 The withdrawal latency was obtained using a Hargreaves apparatus. ####
 File: Intrinsic\_Excitability.csv **Description:** Number of evoked action
 potentials elicited by increasing current injection steps. Recordings were
 obtained from prelimbic neurons. ##### Variables * Sex: Sex of the rat. *
 Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
 * Tx Group: Air (control) or AIE (adolescent intermittent ethanol) * Step
 (pA): Amount of current injected. The duration of current injection was 1
 second. * prl_pag: Number of action potentials generated by a prelimbic
 pyramidal neuron projecting to the ventrolateral periaqueductal gray in
 response to an injection of a known amount of current.  * pvin: Number of
 action potentials generated by a prelimbic parvalbumin positive
 interneuron in response to an injection of a known amount of current. 
 #### File: aEPSCs.csv **Description:** Monosynaptic optically evoked
 asynchronous excitatory postsynaptic currents. Slice electrophysiology
 recordings were obtained from prelimbic neurons while optically
 stimulating the terminals of neurons projecting to the prelimbic cortex
 from the basolateral amygdala. ##### Variables * Sex: Sex of the rat. *
 Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
 * Tx Group: Air (control) or AIE (adolescent intermittent ethanol) *
 Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the
 ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive
 interneuron). * peak (pA): Average peak amplitude of the aEPSCs. *
 interevent interval (ms): Average interevent interval of the aEPSCs. ####
 File: Excitation-Inhibition\_Ratio.csv **Description:** Polysynaptic
 optically evoked excitatory (recorded while holding the cell at +70 mV)
 and inhibitory (recorded while holding the cell at +10 mV) postsynaptic
 currents at prelimbic neurons. These currents were obtained by optically
 stimulating terminals from the basolateral amygdala. ##### Variables *
 Sex: Sex of the rat. * Challenge Group: Saline (control) or carrageenan
 (inflammatory paw pain). * Tx Group: Air (control) or AIE (adolescent
 intermittent ethanol) * Neuron Type: PRL-PAG (Prelimbic pyramidal neuron
 projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic
 parvalbumin positive interneuron). * oEPSC (pA): Peak amplitude of the
 optically evoked excitatory postsynaptic current. * oIPSC (pA): Peak
 amplitude of the optically evoked inhibitory postsynaptic current. * E/I
 Ratio: Ratio of the oEPSC/oIPSC peak amplitudes.