DOI: 10.22002/mmv0p-xth61
title: NHP2L1 siRNA virus particles

description: HIV particles from producer cells treated with NHP2L1 siRNA
description: <strong>Tilt Series Date:</strong> 2012-09-17</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Gaelle Mercenne (Sundquist lab) generated virus. Viral particles were shipped to Caltech to be frozen and imaged by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Virus made in presence of NHP2L1 siRNA</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-09-17-1/3dimage_69443/</p>12sep17_c141_2_AT1BT1_0001_BIN_full.rec,  Reconstruction (Pixel Size 2.0 nm), 593.6 MB  
        
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        12sep17_c141_2_AT1BT1_0001_BIN.st,  Tilt Series (Pixel Size 0.5 nm), 862.2 MB  
        
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        c141_2_0001_particle_2.tiff,  file associated to 3D Image #69443, 672.1 kB  
        
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        c141_2_0001_particle_1.tiff,  file associated to 3D Image #69443, 1.0 MB  
        
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        12sep17_c141_2_AT1BT1_0001_BIN_full.tiff,  file associated to 3D Image #69443, 943.1 kB  
        
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        12sep17_c141_2_AT1BT1_0001_BIN_full_b.tiff,  file associated to 3D Image #69443, 855.3 kB  
        
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