DOI: 10.5061/dryad.j3tx95xqn
title: Data from: <em>In vitro</em> anti-tick effect of <em>Eucalyptus globulus</em> oil and its nano-emulsion against unfed adults of the brown dog tick <em>Rhipicephalus sanguineus</em> sensu lato (Acari: Ixodidae)

description: Rhipicephalus sanguineus Latreille (Ixodida: Ixodidae) has an economic
 impact as it is a blood-feeding ectoparasite transmitting numerous
 pathogens to humans and other animals. The present study evaluated the
 acaricidal effect of eucalyptus essential oil (EO) with the concentrations
 (40 %, 30 %, 20 %, 10 %, 5 %, and 2.5 %), eucalyptus nano-emulsion (ENE)
 with the concentrations (30 %, 20 %, 15 %, 10 %, 5 %, and 2.5 %),
 eucalyptus nano-emulsion combined with silver nanoparticles (ENE &
 SNPs) with the concentrations (5 %, 4%, 3 %, 2 %, and 1 %) and silver
 nanoparticles (SNPs) with the concentrations (5 %, 4 %, 3 %, 2 %, and 1 %)
 on unfed adults of R. sanguineus sensu lato using the adult immersion
 test. There were two controls, one with tween 80 and the other without
 tween 80, while Bravecto® was used as an acaricidal reference. The
 nanoformulations were analyzed by particle size, UV-visible spectroscopy,
 and transmission electron microscopy (TEM). The mortality of treated R.
 sanguineus was recorded daily for 7 days. The mortality of ENE &
 SNPs, EO, and SNPs at 7 days was 96.6%, 90%, and 80%, at concentrations of
 5 %, 40 %, and 5 %, respectively. The lethal concentration to 50%
 mortality (LC50) of EO, ENE, ENE & SNPs, and SNPs at 7 days was
 6.65, 9.49, 2.23, and 4.08%, respectively. The particle size of
 nanoformulations was (83.08, 10.59, and 223.4 nm) for ENE, a mixture of
 SNPs, and ENE & SNPs, respectively. Scanning electron microscopy
 of treated adult R. sanguineus revealed numerous alterations in aeropyles
 of spiracular plates, anal grooves, and mouthparts. The histopathological
 examination of the treated adult R. sanguineus showed degeneration in the
 cuticle, midgut, and salivary gland. These findings demonstrate that
 eucalyptus oil could be used as part of an integrated pest management
 program for the control of R. sanguineus.
description: # Data from: *In vitro* anti-tick effect of *Eucalyptus globulus* oil and
 its nano-emulsion against unfed adults of the brown dog tick
 *Rhipicephalus sanguineus* sensu lato (Acari: Ixodidae)
 [https://doi.org/10.5061/dryad.j3tx95xqn](https://doi.org/10.5061/dryad.j3tx95xqn) ## Description of the data and file structure One-way ANOVA test was used to analyze the data, then Tukey’s test using SPSS program v.20. Regression equation analysis was used to compute the values of lethal concentration to 50% mortality (LC50) based on the mortality data that had been probit converted. The probit approach was utilized to examine the concentration-response data. ### Files and variables #### File: Table_3-6_Bioassay_raw_data_INPOUT.sav **Description:** Raw Data of Table 3 #### File: Table_3_Eucaliptus_oil_OUTPUT.xls **Description:** analysis of Table 3 #### File: Table_4_Eucaliptus_nanoemulsion_OUTPUT.xls **Description:** analysis of Table 4 #### File: Table_5_Eucaliptus_nanoemulsion_with_silver_OUTPUT.xls **Description:** Data of table 5 #### File: Table_7_LC50_treatment_raw_data_INPUT.sav **Description:** Data of table 7  #### File: Table_6_sIlver_nanoparticles_OUTPUT.xls **Description:**  Data of table 6  #### File: Table_7__LC50_on_bilogical_parameters_OUTPUT.xls **Description:** analysis Data of table 7  #### File: TABLE_2._Mortality_percentages_(mean±SE)_of_unfed_Rhipicephalus_sanguineus_adults_treated_with_eucalyptus_oil_(EO)_through_a_week_post_immersion.md **Description:** This file provides detailed documentation of the eucalyptus oil bioassay results. It includes: Overview of the study design and treatment groups.Descriptive statistics (mean, standard deviation, confidence intervals).ANOVA results for significance testing.Post Hoc Tukey HSD comparisons among concentrations. Mortality percentages across 7 days for all treatments. #### File: TABLE_3._Mortality_percentages_(mean±SE)_of_unfed_Rhipicephalus_sanguineus_adults_treated_with_eucalyptus_nano-emulsion_(ENE)_through_a_week_post-treatment[...].md **Description:** This file documents the bioassay results for eucalyptus nanoemulsion treatments. It includes: Overview of the study design and methodology. Treatment groups (control, various nanoemulsion concentrations, Bravecto). Measured parameters (mortality % over 7 days, statistical significance).Statistical outputs: Descriptive statistics, ANOVA tables, and Tukey HSD post hoc tests. #### File: TABLE_4.Mortality_percentages_(mean±SE)_of_unfed_Rhipicephalus_sanguineus_adults_treated_with_eucalyptus_nano-emulsion_combined_with_silver_nanoparticles_(ENE_SNPs)_through_a_week_post_immersion[...].md **Description:** This file documents the bioassay results for eucalyptus nanoemulsion combined with silver. It includes: Overview of the study design and methodology. Treatment groups (control, various concentrations of eucalyptus nanoemulsion with silver, and reference treatment). Measured parameters: mortality percentage across multiple days. Statistical outputs: Descriptive statistics, ANOVA tables, and Tukey HSD post hoc tests. #### File: TABLE_5._Mortality_percentages_(mean±SE)_of_unfed_Rhipicephalus_sanguineus_adults_treated_with_silver_nanoparticles_(SNPs)_through_a_week_post_immersion.md **Description:** This file summarizes the bioassay results for silver nanoparticles. It includes: Overview of the study design and methodology. Treatment groups (control, various concentrations of silver nanoparticles, and Bravecto as reference). Measured parameters: mortality percentage over 7 days. Statistical outputs: Descriptive statistics, ANOVA tables, and Tukey HSD post hoc tests. #### File: TABLE_6._Reproductive_performance_of_Rhipicephalus_sanguineus_females_treated_with_lethal_concentration_to_50__mortality_(LC50)_of_eucalyptus_oil_(EO)__eucalyptus_nanoemulsion_(ENE)__eucalyptus_nanoemulsion_combined_with[...].md **Description:** This file summarizes the LC50 biological parameters analysis. It includes: Overview of the study and methodology for determining LC50 values. Treatment groups (control and various concentrations).Measured parameters such as mortality rates, weight of engorged females, egg mass weight, reproductive index, egg number, hatching percentage, oviposition period, and incubation period. Statistical outputs: Descriptive statistics, ANOVA results, and Tukey HSD post hoc tests.