DOI: 10.5061/dryad.8sf7m0cwr
title: Vegetation variables and rhizosphere bacterial communities from ten paired sites in southwestern Finland, 2021

description: Plant invasions can have major impacts on ecosystems not only in
 aboveground but also in belowground through changes in microbiota.
 Particularly legumes, which often host nitrogen-fixing symbionts
 (rhizobia), can be expected to modify soil bacterial communities. We
 examined the effect of the invasive herbaceous legume, Lupinus
 polyphyllus, on the alpha diversity and community composition of soil
 bacteria. We also explored whether vegetation cover, the cover of other
 (non-invasive) legumes or number of vascular plants was associated with
 soil bacterial communities. We sampled rhizosphere soil and surveyed
 vegetation from ten paired sites (invaded more than ten years ago and
 uninvaded locations) in southwestern Finland, and identified bacterial DNA
 by using 16S rRNA gene amplicon sequencing. The plant invader and the
 three vegetation variables considered had no effect on soil bacterial
 alpha diversity in terms of bacterial richness, Shannon and Inverse
 Simpson diversity indices. The composition of soil bacterial communities
 differed between invaded and uninvaded soils at four out of the ten sites.
 However, the differences in the relative abundances of top bacterial
 families between the two soil origins were inconsistent across sites,
 including rhizobia in the family of Bradyrhizobiaceae that the study
 species is hosting. Moreover, vegetation cover, legume cover after
 excluding the study species, and number of plant species explained a small
 proportion of the variation in the bacterial community composition. Our
 findings indicate that L. polyphyllus has the potential to modify the soil
 bacterial community composition locally, at least at sites where it has
 been present for more than a decade.
description: To examine the impact of the herbaceous legume <em>Lupinus
 polyphyllus</em> on soil bacterial communities, vegetation variables
 and rhizosphere samples were collected from ten paired sites (ten invaded
 and ten uninvaded locations) around the Turku area in southwestern Finland
 in June 2021. The uninvaded locations were at least 30 m apart from the
 invaded locations, and the distances between the ten sample sites varied
 from 1.7 to 32.7 km. Each location contained four sampling points of 1 × 1
 m. Bacterial DNA was identified by using 16S rRNA gene
 amplicon sequencing, resulting in 9897 amplicon sequence variants (ASVs)
 for analysis. To characterise soil bacterial diversity, bacterial richness
 (i.e., number of bacterial ASVs per sample), Shannon and inverse Simpson
 diversity indices were calculated based on the bacterial ASVs.
description: This Community_data2021_README file was generated on 2024-01-09 by Satu
 Ramula () 1. Title of Dataset: Vegetation
 variables and rhizosphere bacterial communities from ten paired sites in
 southwestern Finland, 2021 2. Date of data collection: June 2021 (range:
 2021-06-07 - 2021-06-10) 3. Geographic location of data collection: Turku
 area, Finland (latitude: 60.357-60.521, longitude: 22.168-22.740) 4.
 Information about funding sources that supported the collection of the
 data: Research Council of Finland (#331046) 5. Description of methods used
 for collection/generation of data: To examine the impact of the invasive
 herbaceous legume Lupinus polyphyllus on soil bacterial communities,
 vegetation variables and rhizosphere samples were collected from ten
 paired sites (ten invaded and ten uninvaded locations) around the Turku
 area in southwestern Finland in June 2021. The uninvaded locations were at
 least 30 m apart from the invaded locations, and the distances between the
 ten sample sites varied from 1.7 to 32.7 km. Each location contained four
 sampling points of 1 x 1 m. Bacterial DNA was identified by using 16S rRNA
 gene amplicon sequencing, resulting in 9897 amplicon sequence variants
 (ASVs) for final analysis. To characterise soil bacterial alpha diversity,
 bacterial richness (i.e., number of bacterial ASVs per sample), Shannon
 (H) and inverse Simpson diversity (1/D) indices were calculated based on
 the bacterial ASVs. 6. Data files: A data file contains all variables used
 for the analyses presented in Mousavi SA and Ramula S "The invasive
 legume Lupinus polyphyllus has minor site-specific impacts on the
 composition of soil bacterial communities", submitted for Ecology and
 Evolution ## Data ### Community_data2021.xls Vegetation variables and
 rhizosphere bacterial communities from invaded and uninvaded locations at
 ten sites. The invaded locations refer to invasions by the herbaceous
 legume Lupinus polyphyllus. Variable list: Sample - a unique sample code
 for the recorded variables (4 samples per soil origin at each site, 80
 samples in total) Soil.origin - two levels (Invaded by Lupinus
 polyphyllus, Uninvaded) Site - ten levels (range: A-J) Replicate - 4
 samples per soil origin at each site (range: 1-4) Distance.road.m -
 distance of the sample from the closest main road in m Vegetation.cover -
 total vegetation cover per quadrat (range: 0-1) Legume.cover - legume
 cover per quadrat after excluding L. polyphyllus (range: 0-1)
 No.plant.species - number of vascular plant species per quadrat
 Lupine.cover -cover of L. polyphyllus per quadrat (range: 0-1)
 Bacterial.richness - number of bacterial ASVs per sample Shannon - Shannon
 diversity index (H) calculated from the bacterial ASVs Inverse.Simpson -
 inverse Simpson diversity index (1/D) calculated from the bacterial ASVs
 ASV1-ASV11197 - columns containing relative abundances of bacterial ASVs
 (range: 0-1), 9897 ASVs in total