cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1568612.v2
title: Economic resources consumption structure in severe hypoglycemia episodes: a systematic review and meta-analysis

description: Diabetes mellitus (DM) is associated with severe hypoglycemia events (SHEs) that vary in severity and resource consumption. Here we perform a systematic review in Medline of studies evaluating SHE-related health resource use. Eligible studies investigated patients with DM and included ≥10 SHEs. We also assessed studies identified in another systematic review, and through references from the included studies. We identified 14 relevant studies and used data from 11 (encompassing 6075 patients). Study results were interpreted to fit our definitions, which sometimes required assumptions. SHE type structure was synthesized using Bayesian modeling. Estimating Type 1 & 2 DM separately revealed only small differences; therefore, we used joint results. Of the analyzed SHEs, 9.97% were hospital-treated, 22.3% medical professional-treated, and 67.73% family-treated. These meta-analysis results help in understanding the structure of resource consumption following SHE and can be used in economic studies.
DOI: 10.6084/m9.figshare.1568612.v1
title: Economic resources consumption structure in severe hypoglycemia episodes: a systematic review and meta-analysis

description: Diabetes mellitus (DM) is associated with severe hypoglycemia events (SHEs) that vary in severity and resource consumption. Here we perform a systematic review in Medline of studies evaluating SHE-related health resource use. Eligible studies investigated patients with DM and included ≥10 SHEs. We also assessed studies identified in another systematic review, and through references from the included studies. We identified 14 relevant studies and used data from 11 (encompassing 6075 patients). Study results were interpreted to fit our definitions, which sometimes required assumptions. SHE type structure was synthesized using Bayesian modeling. Estimating Type 1 & 2 DM separately revealed only small differences; therefore, we used joint results. Of the analyzed SHEs, 9.97% were hospital-treated, 22.3% medical professional-treated, and 67.73% family-treated. These meta-analysis results help in understanding the structure of resource consumption following SHE and can be used in economic studies.
DOI: 10.6084/m9.figshare.1568592
title: Genetic diversity in cytochrome c oxidase I gene of <i>Anopheles</i> mosquitoes

description: Genetic diversity in cytochrome c oxidase I (coxI) among 7 species of <i>Anopheles</i> mosquitoes from Pakistan, and 37 species from different geographical regions of the world, was recorded. Automatic Barcode Gap Discovery (ABGD) analysis revealed a clear gap between intraspecific and interspecific distances of 7 species from Pakistan. However, genetic distances of 37 <i>Anopheles</i> species failed to adequately differentiate species in a global context. Intraspecific and interspecific divergences for 7 <i>Anopheles</i> species of Pakistan varied from 0.0% to 2.5% (mean = 0.49%) and 8% to 22.3% (mean = 12.77%), respectively. Similarly, intraspecific distances for 37 species from different parts of world ranged from 0.0% to 11.2% (mean = 0.65%) while values of interspecific divergences ranged from 3.4% to 35% (mean = 11.75%). Although phylogenetic tree revealed separate clades for 7 <i>Anopheles</i> species of Pakistan, it failed to produce separate clades for 37 species of the world. It is concluded that although standard barcode region is helpful for identifying <i>Anopheles</i> mosquitoes, combination of multi-locus approaches and morphology may be required to accurately identify species in this genus.
DOI: 10.6084/m9.figshare.1568591
title: Identification of cardiac stem cells within mature cardiac myocytes

description: Cardiac stem cells are described in a number of mammalian species including humans. Cardiac stem cell clusters consisting of both lineage-negative and partially committed cells are generally identified between contracting cardiac myocytes. In the present study, c-kit<sup>+</sup>, Sca<sup>+</sup>, and Isl1<sup>+</sup> stem cells were revealed to be located <i>inside</i> the sarcoplasm of cardiac myocytes in myocardial cell cultures derived from newborn, 20-, and 40-day-old rats. Intracellularly localized cardiac stem cells had a coating or capsule with a few pores that opened into the host cell sarcoplasm. The similar structures were also identified in the suspension of freshly isolated myocardial cells (ex vivo) of 20- and 40-day-old rats. The results from this study provide direct evidence for the replicative division of encapsulated stem cells, followed by their partial cardiomyogenic differentiation. The latter is substantiated by the release of multiple transient amplifying cells following the capsule rupture. In conclusion, functional cardiac stem cells can reside not only exterior to but also within cardiomyocytes.
DOI: 10.6084/m9.figshare.1568592.v2
title: Genetic diversity in cytochrome c oxidase I gene of <i>Anopheles</i> mosquitoes

description: Genetic diversity in cytochrome c oxidase I (coxI) among 7 species of <i>Anopheles</i> mosquitoes from Pakistan, and 37 species from different geographical regions of the world, was recorded. Automatic Barcode Gap Discovery (ABGD) analysis revealed a clear gap between intraspecific and interspecific distances of 7 species from Pakistan. However, genetic distances of 37 <i>Anopheles</i> species failed to adequately differentiate species in a global context. Intraspecific and interspecific divergences for 7 <i>Anopheles</i> species of Pakistan varied from 0.0% to 2.5% (mean = 0.49%) and 8% to 22.3% (mean = 12.77%), respectively. Similarly, intraspecific distances for 37 species from different parts of world ranged from 0.0% to 11.2% (mean = 0.65%) while values of interspecific divergences ranged from 3.4% to 35% (mean = 11.75%). Although phylogenetic tree revealed separate clades for 7 <i>Anopheles</i> species of Pakistan, it failed to produce separate clades for 37 species of the world. It is concluded that although standard barcode region is helpful for identifying <i>Anopheles</i> mosquitoes, combination of multi-locus approaches and morphology may be required to accurately identify species in this genus.
DOI: 10.6084/m9.figshare.1568592.v1
title: Genetic diversity in cytochrome c oxidase I gene of <i>Anopheles</i> mosquitoes

description: Genetic diversity in cytochrome c oxidase I (coxI) among 7 species of <i>Anopheles</i> mosquitoes from Pakistan, and 37 species from different geographical regions of the world, was recorded. Automatic Barcode Gap Discovery (ABGD) analysis revealed a clear gap between intraspecific and interspecific distances of 7 species from Pakistan. However, genetic distances of 37 <i>Anopheles</i> species failed to adequately differentiate species in a global context. Intraspecific and interspecific divergences for 7 <i>Anopheles</i> species of Pakistan varied from 0.0% to 2.5% (mean = 0.49%) and 8% to 22.3% (mean = 12.77%), respectively. Similarly, intraspecific distances for 37 species from different parts of world ranged from 0.0% to 11.2% (mean = 0.65%) while values of interspecific divergences ranged from 3.4% to 35% (mean = 11.75%). Although phylogenetic tree revealed separate clades for 7 <i>Anopheles</i> species of Pakistan, it failed to produce separate clades for 37 species of the world. It is concluded that although standard barcode region is helpful for identifying <i>Anopheles</i> mosquitoes, combination of multi-locus approaches and morphology may be required to accurately identify species in this genus.
DOI: 10.6084/m9.figshare.1568588
title: Using checklists and algorithms to improve qualitative exposure judgment accuracy

description: Most exposure assessments are conducted without the aid of robust personal exposure data and are based instead on qualitative inputs such as education and experience, training, documentation on the process chemicals, tasks and equipment, and other information. Qualitative assessments determine whether there is any follow-up, and influence the type that occurs, such as quantitative sampling, worker training, and implementing exposure and risk management measures. Accurate qualitative exposure judgments ensure appropriate follow-up that in turn ensures appropriate exposure management. Studies suggest that qualitative judgment accuracy is low. A qualitative exposure assessment Checklist tool was developed to guide the application of a set of heuristics to aid decision making. Practicing hygienists (n = 39) and novice industrial hygienists (n = 8) were recruited for a study evaluating the influence of the Checklist on exposure judgment accuracy. Participants generated 85 pre-training judgments and 195 Checklist-guided judgments. Pre-training judgment accuracy was low (33%) and not statistically significantly different from random chance. A tendency for IHs to underestimate the true exposure was observed. Exposure judgment accuracy improved significantly (p &lt;0.001) to 63% when aided by the Checklist. Qualitative judgments guided by the Checklist tool were categorically accurate or over-estimated the true exposure by one category 70% of the time. The overall magnitude of exposure judgment precision also improved following training. Fleiss’ κ, evaluating inter-rater agreement between novice assessors was fair to moderate (κ = 0.39). Cohen's weighted and unweighted κ were good to excellent for novice (0.77 and 0.80) and practicing IHs (0.73 and 0.89), respectively. Checklist judgment accuracy was similar to quantitative exposure judgment accuracy observed in studies of similar design using personal exposure measurements, suggesting that the tool could be useful in developing informed priors and further demonstrating its usefulness in producing accurate qualitative exposure judgments.
DOI: 10.6084/m9.figshare.1568588.v1
title: Using checklists and algorithms to improve qualitative exposure judgment accuracy

description: Most exposure assessments are conducted without the aid of robust personal exposure data and are based instead on qualitative inputs such as education and experience, training, documentation on the process chemicals, tasks and equipment, and other information. Qualitative assessments determine whether there is any follow-up, and influence the type that occurs, such as quantitative sampling, worker training, and implementing exposure and risk management measures. Accurate qualitative exposure judgments ensure appropriate follow-up that in turn ensures appropriate exposure management. Studies suggest that qualitative judgment accuracy is low. A qualitative exposure assessment Checklist tool was developed to guide the application of a set of heuristics to aid decision making. Practicing hygienists (n = 39) and novice industrial hygienists (n = 8) were recruited for a study evaluating the influence of the Checklist on exposure judgment accuracy. Participants generated 85 pre-training judgments and 195 Checklist-guided judgments. Pre-training judgment accuracy was low (33%) and not statistically significantly different from random chance. A tendency for IHs to underestimate the true exposure was observed. Exposure judgment accuracy improved significantly (p &lt;0.001) to 63% when aided by the Checklist. Qualitative judgments guided by the Checklist tool were categorically accurate or over-estimated the true exposure by one category 70% of the time. The overall magnitude of exposure judgment precision also improved following training. Fleiss’ κ, evaluating inter-rater agreement between novice assessors was fair to moderate (κ = 0.39). Cohen's weighted and unweighted κ were good to excellent for novice (0.77 and 0.80) and practicing IHs (0.73 and 0.89), respectively. Checklist judgment accuracy was similar to quantitative exposure judgment accuracy observed in studies of similar design using personal exposure measurements, suggesting that the tool could be useful in developing informed priors and further demonstrating its usefulness in producing accurate qualitative exposure judgments.
DOI: 10.6084/m9.figshare.1568591.v1
title: Identification of cardiac stem cells within mature cardiac myocytes

description: Cardiac stem cells are described in a number of mammalian species including humans. Cardiac stem cell clusters consisting of both lineage-negative and partially committed cells are generally identified between contracting cardiac myocytes. In the present study, c-kit<sup>+</sup>, Sca<sup>+</sup>, and Isl1<sup>+</sup> stem cells were revealed to be located <i>inside</i> the sarcoplasm of cardiac myocytes in myocardial cell cultures derived from newborn, 20-, and 40-day-old rats. Intracellularly localized cardiac stem cells had a coating or capsule with a few pores that opened into the host cell sarcoplasm. The similar structures were also identified in the suspension of freshly isolated myocardial cells (ex vivo) of 20- and 40-day-old rats. The results from this study provide direct evidence for the replicative division of encapsulated stem cells, followed by their partial cardiomyogenic differentiation. The latter is substantiated by the release of multiple transient amplifying cells following the capsule rupture. In conclusion, functional cardiac stem cells can reside not only exterior to but also within cardiomyocytes.
DOI: 10.6084/m9.figshare.1568591.v3
title: Identification of cardiac stem cells within mature cardiac myocytes

description: Cardiac stem cells are described in a number of mammalian species including humans. Cardiac stem cell clusters consisting of both lineage-negative and partially committed cells are generally identified between contracting cardiac myocytes. In the present study, c-kit<sup>+</sup>, Sca<sup>+</sup>, and Isl1<sup>+</sup> stem cells were revealed to be located <i>inside</i> the sarcoplasm of cardiac myocytes in myocardial cell cultures derived from newborn, 20-, and 40-day-old rats. Intracellularly localized cardiac stem cells had a coating or capsule with a few pores that opened into the host cell sarcoplasm. The similar structures were also identified in the suspension of freshly isolated myocardial cells (ex vivo) of 20- and 40-day-old rats. The results from this study provide direct evidence for the replicative division of encapsulated stem cells, followed by their partial cardiomyogenic differentiation. The latter is substantiated by the release of multiple transient amplifying cells following the capsule rupture. In conclusion, functional cardiac stem cells can reside not only exterior to but also within cardiomyocytes.
DOI: 10.6084/m9.figshare.1568591.v2
title: Identification of cardiac stem cells within mature cardiac myocytes

description: Cardiac stem cells are described in a number of mammalian species including humans. Cardiac stem cell clusters consisting of both lineage-negative and partially committed cells are generally identified between contracting cardiac myocytes. In the present study, c-kit<sup>+</sup>, Sca<sup>+</sup>, and Isl1<sup>+</sup> stem cells were revealed to be located <i>inside</i> the sarcoplasm of cardiac myocytes in myocardial cell cultures derived from newborn, 20-, and 40-day-old rats. Intracellularly localized cardiac stem cells had a coating or capsule with a few pores that opened into the host cell sarcoplasm. The results from this study provide direct evidence for the replicative division of encapsulated stem cells, followed by their partial cardiomyogenic differentiation. The latter is substantiated by the release of multiple transient amplifying cells following the capsule rupture. In conclusion, functional cardiac stem cells can reside not only exterior to but also within cardiomyocytes.
DOI: 10.6084/m9.figshare.1568579
title: Challenges facing European agriculture and possible biotechnological solutions

description: Agriculture faces many challenges to maximize yields while it is required to operate in an environmentally sustainable manner. In the present study, we analyze the major agricultural challenges identified by European farmers (primarily related to biotic stresses) in 13 countries, namely Belgium, Bulgaria, the Czech Republic, France, Germany, Hungary, Italy, Portugal, Romania, Spain, Sweden, UK and Turkey, for nine major crops (barley, beet, grapevine, maize, oilseed rape, olive, potato, sunflower and wheat). Most biotic stresses (BSs) are related to fungi or insects, but viral diseases, bacterial diseases and even parasitic plants have an important impact on yield and harvest quality. We examine how these challenges have been addressed by public and private research sectors, using either conventional breeding, marker-assisted selection, transgenesis, cisgenesis, RNAi technology or mutagenesis. Both national surveys and scientific literature analysis followed by text mining were employed to evaluate genetic engineering (GE) and non-GE approaches. This is the first report of text mining of the scientific literature on plant breeding and agricultural biotechnology research. For the nine major crops in Europe, 128 BS challenges were identified with 40% of these addressed neither in the scientific literature nor in recent European public research programs. We found evidence that the private sector was addressing only a few of these “neglected” challenges. Consequently, there are considerable gaps between farmer’s needs and current breeding and biotechnology research. We also provide evidence that the current political situation in certain European countries is an impediment to GE research in order to address these agricultural challenges in the future. This study should also contribute to the decision-making process on future pertinent international consortia to fill the identified research gaps.
DOI: 10.6084/m9.figshare.1568579.v2
title: Challenges facing European agriculture and possible biotechnological solutions

description: Agriculture faces many challenges to maximize yields while it is required to operate in an environmentally sustainable manner. In the present study, we analyze the major agricultural challenges identified by European farmers (primarily related to biotic stresses) in 13 countries, namely Belgium, Bulgaria, the Czech Republic, France, Germany, Hungary, Italy, Portugal, Romania, Spain, Sweden, UK and Turkey, for nine major crops (barley, beet, grapevine, maize, oilseed rape, olive, potato, sunflower and wheat). Most biotic stresses (BSs) are related to fungi or insects, but viral diseases, bacterial diseases and even parasitic plants have an important impact on yield and harvest quality. We examine how these challenges have been addressed by public and private research sectors, using either conventional breeding, marker-assisted selection, transgenesis, cisgenesis, RNAi technology or mutagenesis. Both national surveys and scientific literature analysis followed by text mining were employed to evaluate genetic engineering (GE) and non-GE approaches. This is the first report of text mining of the scientific literature on plant breeding and agricultural biotechnology research. For the nine major crops in Europe, 128 BS challenges were identified with 40% of these addressed neither in the scientific literature nor in recent European public research programs. We found evidence that the private sector was addressing only a few of these “neglected” challenges. Consequently, there are considerable gaps between farmer’s needs and current breeding and biotechnology research. We also provide evidence that the current political situation in certain European countries is an impediment to GE research in order to address these agricultural challenges in the future. This study should also contribute to the decision-making process on future pertinent international consortia to fill the identified research gaps.
DOI: 10.6084/m9.figshare.1568576
title: The postcranial anatomy of <i>Yacarerani boliviensis</i> and the phylogenetic significance of the notosuchian postcranial skeleton

description: ABSTRACTIn this contribution, we describe new specimens of <i>Yacarerani boliviensis</i> from the Cajones Formation (Upper Cretaceous) of Bolivia. We focus on the postcranial anatomy of <i>Y. boliviensis</i>, because this anatomical region is completely unknown in other sphagesaurid notosuchians up to the present. At least eight individuals representing almost the entire postcranial skeleton are described. Although the postcranial anatomy of <i>Yacarerani</i> resembles that of other notosuchians in many features, five autapomorphic characters were identified: pedicles of the atlas bearing a lateral bulge; absence of rounded depressions on the dorsal surface of the anterior to middle dorsal vertebrae; coracoid bearing an oblique crest on its lateral surface; lateromedially compressed anterior ungual phalanges; and femur with a shallow depression for the M. puboischiofemoralis internus 1 and M. caudifemoralis longus. Observed variation in the postcranial anatomy of basal mesoeucrocodylians was incorporated as new characters in a phylogenetic analysis, expanding the postcranial information used in current phylogenetic data sets. The phylogenetic analysis depicts <i>Yacarerani</i> forming a clade with <i>Adamantinasuchus</i>, which is positioned at the base of Sphagesauridae. This family is well nested among a clade of ’advanced notosuchians,’ and <i>Mariliasuchus</i> is recovered as its sister group. The cladistic analysis recovered new postcranial synapomorphies for Notosuchia and less inclusive clades, although the fact that many taxa lack postcranial remains limits the number of unambiguous postcranial synapomorphies within Notosuchia.SUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVPCitation for this article: Leardi, J. M., D. Pol, F. E. Novas, and M. Suarez Riglos. 2015. The postcranial anatomy of Yacarerani boliviensis and the phylogenetic significance of the notosuchian postcranial skeleton. Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2014.995187.