DOI: 10.5061/dryad.3ffbg79vf
title: Phenotypic measurements of STERILE APETALA mutants in Mimulus verbenaceus

description: In Mimulus verbenaceus, we identified three mutants with abnormal corolla
 development and significantly reduced nectar volume through EMS
 mutagenesis screening, designated as MV00081, MV00017, and NJ00356-1. BSA
 sequencing revealed that MV00081 corresponds to the homolog of the
 Arabidopsis STERILE APETALA. These mutants were named sap-1,
 sap-2, and sap-3, respectively. Among them, sap-1, caused by a premature
 termination mutation, exhibited the most pronounced phenotypic defects
 compared to the other two mutants. We measured leaf, flower, and nectar
 sugar content in sap-1 and found that it significantly inhibited leaf and
 petal development, reduced nectar production, and decreased the fructose
 and glucose content in nectar.
description: # Phenotypic Measurements Dataset ## General Information This dataset
 contains phenotypic measurements of different plant samples. The data
 (Supplemental_Table3.Phenotype_Measurements) is structured into six
 separate sheets, each representing a different aspect of plant morphology
 and physiology. ## Dataset Description ### 1. Nectar Volume *
 **Description**: This sheet records the nectar volume of different plant
 samples. * **Units**: Microliters (μL). * **Columns**: Each column
 represents a different plant sample, including MvBL, MV00081, MV00017, and
 NJ00356-1. ### 2. Leaf Measurements * **Description**: This sheet provides
 measurements of leaf length and width. * **Units**: Millimeters (mm). *
 **Columns**: Each plant sample (e.g., MvBL, MV00081) has two associated
 columns: length and width. ### 3. Flower Width Measurements *
 **Description**: This sheet contains data on the width of different flower
 parts. * **Units**: Millimeters (mm). * **Columns**: * Dorsal Petal Width
 * Lateral Petal Width * Ventral Petal Width * Corolla Tube Width * Data is
 provided for multiple plant samples, including MvBL and MV00081. * **Data
 Organization**: * Each flower was measured for **17 biological
 replicates**. * Since each flower has **two dorsal petals** and **two
 lateral petals**, there are **34 data points** for these measurements. *
 Each flower has only **one ventral petal** and **one corolla tube**,
 resulting in **17 data points** for these measurements. * The lower number
 of data points for ventral petal width and corolla tube width is due to
 the biological structure of the flower, not missing data. ### 4. Flower
 Length Measurements * **Description**: This sheet records the length of
 different floral structures. * **Units**: Millimeters (mm). * **Columns**:
 * Calyx Length * Dorsal Corolla Tube Length (DCT) * Lateral Corolla Tube
 Length (LCT) * Ventral Corolla Tube Length (VCT) * Pistil Length * Stamen
 Length * Ovary Length * Data is provided for multiple plant samples,
 including MvBL and MV00081. ### 5. Sugar Content * **Description**: This
 sheet measures the concentration of sugars in different plant samples. *
 **Units**: Milligrams per milliliter (mg/mL). * **Columns**: * Fructose *
 Glucose * Sucrose * Data is available for samples such as MvBL and sap-1.
 ### 6. Length of 10 Contiguous Cells * **Description**: This sheet records
 the length of 10 contiguous cells in different plant samples. * **Units**:
 Micrometers (μm). * **Columns**: * Cell lengths measured for multiple
 samples, including MvBL and MV00081. * **Handling Missing Data**: *
 Missing data in the dataset is marked as **"null"**. * Some
 missing values are due to biological differences rather than data
 collection issues. For example, wild-type samples typically have 280
 cells, whereas mutant samples have 220 cells. Missing values in these
 cases indicate absent cells in mutants rather than unrecorded data. ### 7.
 Flowering Time * **Description**: This sheet records the number of days
 from sowing to floral bud emergence (defined as the appearance of a
 visible flower bud approximately 1 mm in size), as well as the number of
 leaves at that time point. * **Units**: Days (for flowering time)，Count
 (for leaf number). * **Columns**: * Days to floral bud emergence. * Leaf
 number at floral bud emergence. **File Format** * The dataset is provided
 as an Excel file (.xlsx) containing six sheets. * Each sheet has column
 headers indicating the measurement type and corresponding plant sample.
 **Citation** If using this dataset in your research, please cite
 appropriately. **Contact Information** For further inquiries, please
 contact the dataset creator or the corresponding author: Lisheng
 Zhai zhai.lisheng@njau.edu.cn #### Software: This Leaf.R script reads and
 processes leaf measurement data, summarizes it by treatment and index, and
 generates a bar plot with error bars and significance markers to compare
 leaf traits between two treatments. This mv81_MvBL.R script analyzes
 floral organ length data from two treatments (MvBL and mv81), summarizes
 it by organ type, and generates a bar plot with error bars and
 significance markers to visually compare differences. This mv81_MvBL_2.R
 script processes and summarizes petal width data from two treatments (MvBL
 and mv81), and generates a bar plot with error bars and significance
 markers to compare organ-level differences between treatments.