DOI: 10.5683/sp2/jl7y2i
title: Phenotyping and genotyping of recombinant inbred lines and related parents of common bean (Phaseolus vulgaris L.) segregating for seed protein composition

description: The present dataset includes data acquired in support of a study whose objective was to determine whether a variation in seed protein composition can be used to improve the composition of essential amino acids, particularly the sulphur amino acids, methionine and cysteine, in the seed of common bean (Phaseolus vulgaris L.). The dataset includes a file (Table1_SuppInfo) combining phenotypic data on storage protein profile and amino acid concentrations with genotyping data obtained with the Infinium II BARCBean6K_3 BeadChip (Song et al. 2015 G3 5, 2285; doi:10.1534/g3.115.020594) for a population of recombinant inbred lines derived from a cross between Morden003 and SMARC1N-PN1, along with the following parental and related genotypes: Sanilac, SARC1, SMARC1-PN1, Great Northern US 1400, three Phaseolus coccineus accessions deficient in phaseolin and the wild P. vulgaris accession G12882. The following files are included: Dataset1_SuppInfo. SDS-PAGE of protein profiles of parents and recombinant inbred lines. M: Morden003; S: SMARC1N-PN1. Pop1 is indicated by the prefix F08-18-01 followed by a number; Pop2 by the prefix F08-18-02; and Pop3 by the prefix W09-01-01. The protein genotype is indicated by a color-coded arrow: blue, Morden003 genotype at both phaseolin and lectin loci (MM); green, Morden003 genotype at the phaseolin locus and SMARC1N-PN1 genotype at the lectin locus (MS); red, SMARC1N-PN1 genotype at the phaseolin locus and Morden003 genotype at the lectin locus (SM); and black, SMARC1N-PN1 genotype at both phaseolin and lectin loci (SS). Table1_SuppInfo. Genotypic and phenotypic information of parents, RILs and related genotypes. High-density SNP genotyping information is provided, along with the genotype at the phaseolin and lectin loci, and the concentration of cysteine, methionine, cysteine + methionine and S-methylcysteine. Average; n = 3. Table2_SuppInfo. Concentration of cysteine, methionine, cysteine +methionine and S-methylcysteine for a subgroup of RILs that was determined at the SPARC BioCentre of SickKids Hospital, Toronto, ON. Figure1_SuppInfo. Graphical representation of the genetic map of the Morden003 × SMARC1N-PN1 recombinant inbred population. Table3_SuppInfo. Information on SNP markers used to track phaseolin or lectin deficiency. Top panel provides genotyping results. Lower panel provides allele information. Table4_SuppInfo. Primer information of SNP markers used to track phaseolin or lectin deficiency. Dataset2_SuppInfo. Genotyping data obtained during the validation of marker ss715646356. RFU: relative fluorescence units. Dataset3_SuppInfo. Genotyping data obtained during the validation of marker ss715647037. RFU: relative fluorescence units. Dataset4_SuppInfo. Genotyping data obtained during the validation of marker ss715648961. RFU: relative fluorescence units. Dataset5_SuppInfo. Melt curves of SNP primers used with the (Tm)-shift SNP genotyping method. Dataset6_SuppInfo. Amino acid and protein profiles of each field plot for 7 SS lines at Morden (MF) and London (LF) (2014 and 2015).