cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0d21wg8
title: CIL:38946, Puccinia graminis. In Cell Image Library

description: B0004474 TTS wheat rust uredosori. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Light micrograph of a transverse section through wheat stem showing uredosori of wheat rust (Puccinia graminis). These sori appear as rust-coloured streaks on the wheat. The released uredospores will infect other wheat plants.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: bright-field microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in adsorption or binding of stain
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j09g5knh
title: CIL:38944, mast cell. In Cell Image Library

description: B0004150 Mast cell showing histamine granules. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Colorized transmission electron microscope image of a mast cell full of histamine granules (pink). Histamine is released as part of an allergic reaction. The diameter of the cell is approx. 8.4 micrometers.
description: Preparation: chemically fixed tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Processing history: colorized
DOI: 10.6075/j0kk99ms
title: CIL:38945. In Cell Image Library

description: Colorized scanning electron micrograph showing the stacked membrane discs of the Golgi complex. The Golgi is the area within a cell where many carbohydrates are synthesised, which can be used to modify proteins that pass through the Golgi on the way to other parts of the cell.
description: Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
description: B000434. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j09885v9
title: CIL:38941, Peziza, ascospore. In Cell Image Library

description: B0006300 Ascospores in a Peziza. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: A light micrograph of a thin slice through a cup fungus called Peziza. It grows on decaying wood and organic matter and reproduces itself by producing ascospores. This section shows the fungus' spore containers (asci) each with eight ascospores (shown in brown). As the spores mature, fluid builds up behind them; when the pressure gets too much the tops of the asci open and the spores are flung out into the air. They can cause allergy and irritation in humans including conditions such as dermatitis and hypersensitivity pneumonitis (a condition of the lungs).
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: modes of light microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in intrinsic optical density
Visualization methods: visualization of contiguous regions
DOI: 10.6075/j05d8qpt
title: CIL:38940, Dipsacaceae. In Cell Image Library

description: B0006295 Section through a teasel bud. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: This light micrograph of a section through a teasel bud shows a complex pattern controlled by the same family of genes that controls the layout of animal and human bodies as well. Teasels are thistle-like wild flowers that have long been used in traditional herbal medicine. Teasel root tea was once used to stimulate the appetite, reduce fluid retention and help some liver complaints. Bruised roots were used in an ointment to treat warts, and teasels were even used to ward off witches.
description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: bright-field microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in adsorption or binding of stain
Processing history: unprocessed raw data
DOI: 10.6075/j0v986xx
title: CIL:38814, prostate cancer cells. In Cell Image Library

description: A scanning electron color-enhanced image showing a clump of prostate cancer cells.
description: B0006629 2007 Collection: Medical Photographic Library Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of cont
Processing history: color enhanced
DOI: 10.6075/j08c9vjn
title: CIL:38827, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: a high resolution longitudinal section of four kinetal rows of Coleps showing transverse sections of cilia and basal bodies. Parasomal sacs/clathrin coated pits penetrate through the plasma membrane into the cytoplasm adjacent to each basal body. An accumulation of early endosome-associated vesicles are near each parasomal sac. Cortical tubular mitochondria are evident. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/23/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0wm1cq2
title: CIL:38861, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The raw film was scanned with a Nikon Coolscan 9000ED. This digitized image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: A high resolution view of the cytopharynx but in a dividing Didinium. This cytopharynx is in the proter (anterior) daughter cell. The lamellae, each consisting of a set of perpendicularly arranged microtubules cover this food-vacuole forming region. Vesicles lie near the cytopharyngeal membrane and fuse with the membrane. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X.
DOI: 10.6075/j05m652f
title: CIL:38864, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Lamellae of cytopharynx in the opisthe of a dividing cell. This cytopharynx is not yet functional and lacks most of the medium dense vesicles that accumulate between the lamellae. Toxicysts are present. One toxicyst has myelin-like layers at the level it was sectioned. Bacteria align adjacent to the cell. they may be symbiotic. TEM taken on 5/9/69 by M. Sage with Philips 300 operating at 60kV. Neg. 9,250X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0416wc0
title: CIL:38866, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A high resolution image of a layer of mucocysts docked under the pellicle. The pellicle has a plasma membrane and an alveolar sac system. Both basal bodies of a dikinetid are ciliated, and the food vacuoles are plentiful and filled with bacteria. TEM taken on 6/5/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0qr4wg3
title: CIL:38889, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The anterior end of Euplotes tilts upward and contains triplet rows of cilia which are elements of the AZM. Ridges between the membranelles of the AZM contain mitochondria and electron transparent vesicles, with or without electron opaque granules, lie below the membranelles. The cytosol at the dorsal surface of the cell has a compact layer of mitochondria. TEM taken on 7/29/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,800X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0df6qhb
title: CIL:38830, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A high resolution image of the surface of Coleps hirtus. Alveoli have longitudinal margins along the ciliary rows as well as lateral margins spaced at intervals of one or two basal bodies along the rows. Margins of adjacent alveoli form septa and pores pass through both the lateral and longitudinal septa. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/24/69 by R. Allen with Philips 300 operating at60kV. Print from negative scanned for Photoshop. Neg. 5,000X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0mw2fsf
title: CIL:38855, Arabidopsis thaliana, leaf. In Cell Image Library

description: Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics. Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. See S. Vitha et al. 2001 J Cell Biol 153:111-119.
description: Leaves from wildtype Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ1-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin. The protein is localized at the site of division which occurs by constricting at the chloroplast mid-point. The upper images show the fluorescence signal, the lower panels, the corresponding DIC images.
description: Preparation: formaldehyde fixed tissue; tissue in wax embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of epitope; boundaries between regions with different refractive index
Visualization methods: Fluorescein (FITC)
Data qualification: Processed
DOI: 10.6075/j0wm1c7s
title: CIL:38817, Vaginicola. In Cell Image Library

description: B0005973 Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Processing history: colorized
description: Scanning electron micrograph of a Vaginicola, a single-celled creature found in pond water. The cell (green) secretes and lives within a protective casing called a lorica into which it can contract. The cell is attached to the bottom of the lorica and has hair-like cilia at the end, which it uses for feeding. Scanning electron micrograph 2000 Collection Wellcome Images.
DOI: 10.6075/j0639nk7
title: CIL:38811, blood cell, red blood cell. In Cell Image Library

description: B0006424 2006 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Scanning electron micrograph of red blood cells clearly showing their biconcave disc shape. Human red blood cells are typically 8 microns x 2 microns in size.
description: Preparation: dispersed cells in vitro
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
DOI: 10.6075/j0v40tjp
title: CIL:38825, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity; differences in adsorption or binding of stain
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: High resolution image of the macronucleus of Coleps hirtus. Heterochromatin and nucleoli are seen as smaller and larger stained bodies, respectively, in the macronucleus. Many nuclear pores exist in the nuclear envelope.
description: The material was prepared by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/23/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0rv0n0r
title: CIL:38818, Mus musculus, neuron of the dorsal spinal cord, dorsal root ganglion. In Cell Image Library

description: Dorsal root ganglion cells from embryonic mice were cultured and stimulated to produce cyclooxygenase-2 (COX-2). Cultures were immuno-stained for neuron-associated tubulin (green), COX-2 (red) and DNA (blue). COX-2 is primarily expressed in neurons.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Alexa Fluor 488; Rhodamine; 4',6-diamidino-2-phenylindole (DAPI)
description: After induction of COX-2, cultures were fixed with paraformaldehyde overnight, then immunostained with a mouse antibody to neuronal class III beta-tubulin followed by an Alexa 488-conjugated goat anti-mouse to identify neurons. COX-2 was imaged using Fluorocoxib A, a selected COX-2 inhibitor conjugated to carboxy-X-rhodamine (see http://cancerres.aacrjournals.org/content/70/9/3618.full). Preparations were counterstained with DAPI, and imaged with a Leica CMS microscope using a 40x 0.8 NA objective lens. Images were recorded with a Qimaging Qi CCD camera.
DOI: 10.6075/j0rf5swt
title: CIL:38816, Cavia porcellus, auditory hair cell. In Cell Image Library

description: B0004625 Collection: Wellcome Images Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
description: A scanning electron microscope image of the sensory hair bundle of an inner hair cell from a guinea pig's hearing organ in the inner ear. Vibrations made by sound cause the hairs to be moved back and forth, alternately stimulating and inhibiting the cell. When the cell is stimulated it causes nerve impulses to form in the auditory nerve, sending messages to the brain.
DOI: 10.6075/j0hd7tzc
title: CIL:38860, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Contractile vacuole in the opisthe of a dividing cell. This is presumably the mature CV of the original ‚Äúmother‚Äù cell. Spongiome tubules form large regions of additional membrane on two sides of the CV. The CV contains membrane-like layers that may be lipid. A food vacuole is observed containing expanded trichocysts of Paramecium. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).