DOI: 10.5281/zenodo.14756889
title: Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria

description: Hyperspectral imaging data. All directories contain the hyperspectral imaging data for the corresponding experiments.

ecoli_blvda_pellets_1_and_WT_ctrl.zip: E. coli pYC302 expressing biliverdin IXa cell pellets exposed to varying concentrations of pC-HSL and wildtype E. coli cell pellets. Required to reproduce Figures 3c,d, and e and Supplementary Figure 7.

ecoli_blvda_pellets_2_rg_bchla_pellets_3.zip: E. coli pYC302 expressing biliverdin IXa cell pellets exposed to varying concentrations of pC-HSL and R. gelatinosus YF10 expressing bacteriochlorophyll a cell pellets exposed to varying concentrations of 3-OH-C14-HSL. Required to reproduce Figures 3c,d, and e and Supplementary Figure 7.

ecoli_blvda_ecoli_yfp_pellets_3.zip: E. coli pYC302 expressing biliverdin IXa and strain pYC303 expressing YFP cell pellets exposed to varying concentrations of pC-HSL. Required to reprodtuce Figures 3c,d, and e and Supplementary Figure 7.

rg_wt_aj1_yf6_pellets.zip: R. gelatinosus wild-type, AJ1, and YF6 pellets. Required to reproduce figures 3f and g and Supplementary Figure 7.

rg_yf10_gradient_varied_soils.zip: R. gelatinosus YF6 and AJ1 applied to varied soil types. Required to reproduce figures 3a, b, and h and Extended Figure 6.

pputida_WT_pellets_ctrl.zip: P. putida YC302 wild-type pellet control imaging dataset. Required to reproduce Supplementary Figure 9.

pputida_blvda_pellets_1.zip: P. putida pYC302 expressing biliverdin IXa cell pellets exposed to varying concentrations of pC-HSL. Required to reproduce Supplementary Figure 9.

pputida_blvda_pellets_2.zip: P. putida pYC302 expressing biliverdin IXa cell pellets exposed to varying concentrations of pC-HSL. Required to reproduce Supplementary Figure 9.

pputida_blvda_pellets_3.zip: P. putida pYC302 expressing biliverdin IXa cell pellets exposed to varying concentrations of pC-HSL. Required to reproduce Supplementary Figure 9.

rg_bchla_pellets_ctrl.zip: R. gelatinosus YF10 pellets exposed to varying levels of 3OH-C14-HSL and wild-type and AJ1 pellet control. Required to reproduce Supplementary Figure 13.

rg_bchla_pellets_2.zip: R. gelatinosus YF10 pellets exposed to varying levels of 3OH-C14-HSL. Required to reproduce Supplementary Figure 13.

pputida_blvda_pchsl_sand_1,2.zip: P. putida pYC302 expressing biliverdin IXa on sand with varying concentration of pC-HSL buried under sand. Required to reproduce Figure 4d and Supplementary Figure 12.

pputida_blvda_pchsl_sand_3.zip: P. putida pYC302 expressing biliverdin IXa on sand with varying concentration of pC-HSL buried under sand. Required to reproduce FIgure 4d and Supplementary Figure 12.

pputida_on_sand_iphone.jpg: iPhone camera image of pYC302 expressing biliverdin IXa on sand with varying concentration of pC-HSL buried under sand. Required to reproduce Extended Figure 7.

rg_on_sand_24m.zip: R. gelatinosus YF6 and WT on sand imaged with a UAV from 24 m. Required to reproduce Extended Figure 8.

rg_on_sand_induction_54m.zip: R. gelatinosus YF10 on sand exposed to varying levels of 3-OH-C14-HSL, imaged with a UAV from 54 m. Required to reproduce Figures 4f and g and Extended Figure 9.

rg_in_field_sensing.zip: R. gelatinosus YF10 on sand with 3OH-C14-HSL buried under sand, imaged with a roof-mounted hyperspectral camera. Required to reproduce Figure 4h, i, and j and Extended Figure 10.

rg_in_field_sensing_replicates.zip: Replicate image of R. gelatinosus YF10 on sand with 3OH-C14-HSL buried under sand, imaged with a roof-mounted hyperspectral camera. Required to reproduce Supplementary Figure 14.

ecoli_blvda_filters_colsFGH.zip: E. coli pYC301 applied to filter surfaces at varying concentrations to measure limit of detection. Required to reproduce Extended Figures 3a, b, c, and d.

rg_yf6_filters.zip: R. gelatinosus YF6 applied to filter surfaces at varying concentrations to measure limit of detection. Required to reproduce Extended Figures 3e, f, g, and h.