cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/2cvdg-ze968
title: CenK Sacculus

description: C. crescentus CenK sacculus .
description: <strong>Tilt Series Date:</strong> 2007-05-05</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 2/2, BSA treated 10nm gold, 100% humidity, -4mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-6/rawdata/</p>CenK_07.mrc,  Tilt Series (Pixel Size 0.96 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-6/rawdata/CenK_07.mrc"
        > <i class="download icon"></i> Download </a></p>

        CenK_07.rec,  Reconstruction (Pixel Size 0.96 nm), 299.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-6/3dimage_3018/CenK_07.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6wawv-3rp47
title: Myxococcus xanthus PilO-sfGFP

description: Grid box YWC110Strain SA4088
description: <strong>Tilt Series Date:</strong> 2014-07-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-57.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor/BatchRunTomo</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 31 hr in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.6</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-40/rawdata/</p>20140716_YWC110_50002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-40/rawdata/20140716_YWC110_50002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140716_YWC110_50002_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-40/3dimage_44909/20140716_YWC110_50002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.6mksgn
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Symphyotrichum novi-belgii",
 "Country is Armenia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048413-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.mpsmrv
title: Occurrence Download

description: A dataset containing 135 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Symphyotrichum novi-belgii",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 135 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048414-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/pdh68-r2539
title: CheA-GE8-L2

description: silver binding peptide in linker 2 on CheAData collected on TITAN HHMI
description: <strong>Tilt Series Date:</strong> 2012-10-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: °, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> FEI Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Carly Carter, Dan Feldheim</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB, induced with 14 mM IPTG for 3 hours, lysed with penicillin for 1 hour, add DNAse buffer, DNAse for 20 min, add AgNO3 (0.014g AgNO3 in 1ml HEPES pH 7.2) and 50µl to 1 ml, incubated 20 min, spin down at 6krpm</p>
<strong>Sample Preparation:</strong> plunge freeze on Quantifoil R2/2 in liquid ethane/propane mix</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-26/rawdata/</p>CheA_AG-1_35_orig.st,  Tilt Series (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-26/rawdata/CheA_AG-1_35_orig.st"
        > <i class="download icon"></i> Download </a></p>

        CheA_AG-1_35.rec,  Reconstruction (Pixel Size 0.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-26/3dimage_23732/CheA_AG-1_35.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.424u68
title: Occurrence Download

description: A dataset containing 34 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Symphyotrichum novi-belgii",
 "Country is Belarus",
 "HasCoordinate is true"
 ]
}

 The dataset includes 34 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048415-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/jgx8z-ymx56
title: Caulobacter crescentus

description: wild type CB15N. 3 of 6, 30 degrees missing b/c identical images; very low contrast. Leginon dataset; lightly fixed cells; contrast too low
description: <strong>Tilt Series Date:</strong> 2006-02-08</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 31.0°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; exponential phase; glutaraldehyde-fixed on grid; see DPD notebook Feb 2-3, 2006</p>
<strong>Sample Preparation:</strong> box 177, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1 sec, drain 1.5 sec; see DPD notebook Aug 14, 2006</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-02-08-3/rawdata/</p>06feb08t03.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-02-08-3/rawdata/06feb08t03.mrc"
        > <i class="download icon"></i> Download </a></p>

        06Feb08t03trbn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-02-08-3/3dimage_280/06Feb08t03trbn_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ycyvk-3e827
title: Hylemonella gracilis

description: Hylemonella gracilis.  The rest of the files are in ~/Images/060816.  This date's files are not good enough for motor averaging.
description: <strong>Tilt Series Date:</strong> 2006-08-16</p>
<strong>Data Taken By:</strong> Gavin Murphy</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> Cells provided by Jared Leadbetter; Data collected and processed by Gavin Murphy</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in dilute media without salt.  0.5 g/L of Tryptone, 0.5 g/L YE. Cells grown for 2 days.  OD never gets above 0.005.  Centrifuged lightly, then concentrated 100x fold.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2006-08-16-11/rawdata/</p>S12_S474G3_90e10d22k.mrc,  Tilt Series (Pixel Size 0.98 nm), 964.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2006-08-16-11/rawdata/S12_S474G3_90e10d22k.mrc"
        > <i class="download icon"></i> Download </a></p>

        S12.mrc,  Reconstruction (Pixel Size 0.0 nm), 138.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2006-08-16-11/3dimage_71/S12.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w3qb4-mp476
title: E.coli "strain 2" P221D

description: E.coli "strain 2" P221D chromosomal tsr mutant locked in the CHeA inactive (CCWA) state
description: <strong>Tilt Series Date:</strong> 2012-02-21</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown at 30 degree C in TB/gentamycin to OD600: 0.38.culture was concentrated (x4) and resuspended in TB/gentamycin penicillin. Incubation 1 hour at 30 degrees.</p>
<strong>Sample Preparation:</strong> frozen with Vitrobot settings:-4 offset3.5s blot timeno drain 3 µl sample100% humiditypure ethane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-21-12/rawdata/</p>strain2day10015.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-21-12/rawdata/strain2day10015.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain2day10015_part.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-21-12/3dimage_16399/strain2day10015_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4mmd4-f9h51
title: Hippocampal Neurons (cultured)

description: Plunge frozen at DIV 14
description: <strong>Tilt Series Date:</strong> 2014-08-21</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-12/rawdata/</p>082014_neurons0019.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-12/rawdata/082014_neurons0019.mrc"
        > <i class="download icon"></i> Download </a></p>

        082014_neurons0019_full.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-12/3dimage_45582/082014_neurons0019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.3882n7
title: Occurrence Download

description: A dataset containing 10806 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Malus baccata"
 ]
}

 The dataset includes 10806 records from 287 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048411-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/7hn82-57d28
title: Caulobacter crescentus

description: CB15N-LS101. 1 of 1, very low contrast. non-mutated basic lab strain of Caulobacter; not processed because contrast too low
description: <strong>Tilt Series Date:</strong> 2004-02-16</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Dual Axis, tilt range: (-62.0°, 62.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -2.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> </p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep &data collection: D. Prabha Dias (not processed)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ATCC media (made by A.Frangakis);  0.5ml culture in perforated 1.5ml microfuge tube; extended stationary phase; see DPD notebook Jan 14-16, 2004</p>
<strong>Sample Preparation:</strong> box 79, grid 3; Lacy carbon; 10nm gold on grids & in cell suspension; 6 ul per grid; offset -3, blot 1 sec, no drain time; see DPD notebook Jan 14-16, 2004</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-02-16-1/rawdata/</p>CC_A2_60e_axis1.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-02-16-1/rawdata/CC_A2_60e_axis1.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC_A2_60e_axis2.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-02-16-1/rawdata/CC_A2_60e_axis2.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hnqyc-gkp67
title: E.cloacae-typeV-CdiA+

description: Type V secretion system
description: <strong>Tilt Series Date:</strong> 2014-03-03</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Drs. Davod Low and Chris Hayes, UC Santa Barbara</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning</p>
<strong>Sample Preparation:</strong> Blot time 3s, Blot off-set -4, 3 ul culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-8/rawdata/</p>atlas10008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-8/rawdata/atlas10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10008_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-8/3dimage_39528/atlas10008_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5zvg0-kv613
title: Myxococcus xanthus delta-pilC

description: grid box YWC060
description: <strong>Tilt Series Date:</strong> 2013-12-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-17/rawdata/</p>20131226_Janelia_YWC060_10041.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-17/rawdata/20131226_Janelia_YWC060_10041.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131226_Janelia_YWC060_10041_part131_1.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-17/3dimage_37096/20131226_Janelia_YWC060_10041_part131_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vxrq4-99w23
title: Secretogranin II-YFP

description: Correlated light electron microscopy (CLEM) of secretogranin II-YFP. The aim of the experiment was to determine whether ribosome associated granules contained secretogranin II.
description: <strong>Tilt Series Date:</strong> 2014-08-26</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Secretoganin II-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -25.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Friedberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-26-10/rawdata/</p>tomography3.mrc,  Tilt Series (Pixel Size 0.48 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-26-10/rawdata/tomography3.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography3_part121_1.rec,  Reconstruction (Pixel Size 1.92 nm), 223.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-26-10/3dimage_45815/tomography3_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>