DOI: 10.5061/dryad.p24q3
title: Data from: Pollinator identity and spatial isolation influence multiple paternity in an annual plant

description: The occurrence and extent of multiple paternity is an important component
 of variation in plant mating dynamics. However, links between pollinator
 activity and multiple paternity are generally lacking, especially for
 plant species that attract functionally diverse floral visitors. In this
 study, we separated the influence of two functionally distinct floral
 visitors (hawkmoths and solitary bees) and characterized their impacts on
 multiple paternity in a self-incompatible, annual forb, Oenothera
 harringtonii (Onagraceae). We also situated pollinator-mediated effects in
 a spatial context by linking variation in multiple paternity to variation
 in plant spatial isolation. We documented pronounced differences in the
 number of paternal sires as function of pollinator identity: on average,
 the primary pollinator (hawkmoths) facilitated mating with nearly twice as
 many pollen donors relative to the secondary pollinator (solitary bees).
 This effect was consistent for both isolated and non-isolated individuals,
 but spatial isolation imposed pronounced reductions on multiple paternity
 regardless of pollinator identity. Considering that pollinator abundance
 and pollen dispersal distance did not vary significantly with pollinator
 identity, we attribute variation in realized mating dynamics primarily to
 differences in pollinator morphology and behavior as opposed to pollinator
 abundance or mating incompatibility arising from underlying spatial
 genetic structure. Our findings demonstrate that functionally distinct
 pollinators can have strongly divergent effects on polyandry in plants and
 further suggest that both pollinator identity and spatial heterogeneity
 have important roles in plant mating dynamics.
description: Oenothera harringtonii
 microsatellite genotypesMicrosatellite genotypes
 for parental and offspring individuals included in this study. Each row
 represents a unique O. harringtonii individual whose identifier is given
 in the first column. In the "parents" sheet, the parentID column
 gives the unique identifier for each parental individual. In the
 "offspring" sheet, the offspringID column gives the unique
 identifier for each offspring individual, which consists of the maternal
 identifier followed by an indication of pollinator exclusion treatment (C
 = control, D = day-excluded, N = night-excluded) and an integer that
 distinguishes that individual from others in the same maternal sibship. In
 both sheets, subsequent columns contain allele sizes for each of seven
 diploid loci.genotypes.xlsxpollinator exclusion, spatial isolation and multiple paternityPollinator exclusion treatment, individual-level spatial isolation and correlated paternity estimates for the seed families included in this study. Each row represents a sample of seeds taken from a single fruit. For a given seed family, "plantID" gives the maternal line identifier, "treatment" gives the pollinator exclusion treatment (c = control, de = day-excluded, ne = night-excluded), "isolation20" gives the sum of Euclidean distances (m) between the maternal plant and its twenty nearest neighbors, and "correlatedPaternity" gives the MLTR estimate of correlated paternity.multiplePaternity.csvpollen dispersal distancesPollen dispersal distances analyzed in this study. Each row represents a single seed for which a high-confidence paternity assignment was obtained. For each such seed, "treatment" gives the pollinator exclusion treatment (C = control, DE = day-excluded, NE = night-excluded) and "distance" gives the straight-line distance (m) between the maternal plant and the assigned paternal plant.pollenDispersal.csvmorning pollinator visitationPollinator visitation data for morning observation periods during our study. Each row represents the visitation frequency of a certain type of pollinator during a given observation period. For each observation period, "abundance" gives the number of foraging bouts per observed flower, and "pollinator" gives the pollinator identity (bee or moth).amPollinators.csvevening pollinator visitationPollinator visitation data for evening observation periods during our study. Each row represents the visitation frequency of a certain type of pollinator during a given observation period. For each observation period, "abundance" gives the number of foraging bouts per observed flower, and "pollinator" gives the pollinator identity (bee or moth).pmPollinators.csvcombined pollinator visitationThis file contains the morning and evening pollinator visitation data combined into one file, and was used in the pooled analysis of pollinator abundance.overallPollinators.csvpollinator foraging boutsThis file contains the number of different flowers visited during individual pollinator foraging bouts during our study. Each row represents a unique foraging bout. For each foraging bout, "time" gives the observation time (morning or evening), "visitor" gives the pollinator identity (hawkmoth or solitary bee) and "flowersVisited" gives the number of different flowers visited during that foraging bout.pollinatorBouts.csvdataframe for Figure 2This file contains a simple table used to create Figure 2, which summarizes patterns of pollinator abundance during our study.obs.csvR scriptR script to reproduce analyses and figures presented in this study.Rhodes et al. 2017.R