cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.57760/sciencedb.j00210.00081
title: Raw RNA-seq data of Mingxian169(MX169), Langdon x Ae. mutica amphiploid and four translocation lines
title: Raw RNA-seq data of Mingxian169(MX169), Langdon x Ae. mutica amphiploid and four translocation lines

description: This study comprises a total of six transcriptome sequencing libraries. The sequencing strategy for all libraries is RNA-Seq, with a transcriptomic source. Library construction employed cDNA selection, with a paired-end sequencing layout. Sequencing was performed on the DNBSEQ platform using the DNBSEQ-T7 instrument model. The details for each sample are as follows:Sample Mingxian169(MX169): The corresponding sequencing data files are MX169-1.fq.gz and MX169-2.fq.gz.Sample Langdon-Ae. mutica amphiploid: The corresponding sequencing data files are LDNmutica-1.fq.gz and LDNmutica-2.fq.gz.Sample YW24-788-203: The corresponding sequencing data files are 788-203-1.fq.gz and 788-203-2.fq.gz.Sample YW24-788-309: The corresponding sequencing data files are 788-309-1.fq.gz and 788-309-2.fq.gz.Sample YW24-788-300: The corresponding sequencing data files are 788-300-1.fq.gz and 788-300-2.fq.gz.Sample YW24-788-223: The corresponding sequencing data files are 788-223-1.fq.gz and 788-223-2.fq.gz.All data files are stored in the .fq.gz format.
description: This study comprises a total of six transcriptome sequencing libraries. The sequencing strategy for all libraries is RNA-Seq, with a transcriptomic source. Library construction employed cDNA selection, with a paired-end sequencing layout. Sequencing was performed on the DNBSEQ platform using the DNBSEQ-T7 instrument model. The details for each sample are as follows:Sample Mingxian169(MX169): The corresponding sequencing data files are MX169-1.fq.gz and MX169-2.fq.gz.Sample Langdon-Ae. mutica amphiploid: The corresponding sequencing data files are LDNmutica-1.fq.gz and LDNmutica-2.fq.gz.Sample YW24-788-203: The corresponding sequencing data files are 788-203-1.fq.gz and 788-203-2.fq.gz.Sample YW24-788-309: The corresponding sequencing data files are 788-309-1.fq.gz and 788-309-2.fq.gz.Sample YW24-788-300: The corresponding sequencing data files are 788-300-1.fq.gz and 788-300-2.fq.gz.Sample YW24-788-223: The corresponding sequencing data files are 788-223-1.fq.gz and 788-223-2.fq.gz.All data files are stored in the .fq.gz format.
DOI: 10.15468/dl.79bp7b
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((-93.7629964000000058 32.4310649999999967, -93.7629964000000058 32.4334894100000000, -93.7629964000000058 32.4355905600000014, -93.7629964000000058 32.4384998399999986, -93.7629964000000058 32.4406009900000001, -93.7629964000000058 32.4438335299999991, -93.7629964000000058 32.4472276999999991, -93.7591105999999996 32.4472276999999991, -93.7558721900000052 32.4472276999999991, -93.7516622499999954 32.4472276999999991, -93.7480999999999938 32.4472276999999991, -93.7458333999999951 32.4472276999999991, -93.7458333999999951 32.4428637700000024, -93.7458333999999951 32.4399544899999981, -93.7458333999999951 32.4373684500000010, -93.7458333999999951 32.4344591699999967, -93.7458333999999951 32.4310649999999967, -93.7482619200000045 32.4310649999999967, -93.7527956899999992 32.4310649999999967, -93.7558721900000052 32.4310649999999967, -93.7600821200000070 32.4310649999999967, -93.7629964000000058 32.4310649999999967))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1932",
 "BasisOfRecord is one of (Human Observation, Living Specimen, Observation, Specimen, Machine Observation)"
 ]
}

 The dataset includes 8 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0091922-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.11530374
title: THE PROSPECTS OF UZBEKISTAN'S MEMBERSHIP IN THE WORLD TRADE ORGANIZATION
DOI: 10.5281/zenodo.11530375
title: THE PROSPECTS OF UZBEKISTAN'S MEMBERSHIP IN THE WORLD TRADE ORGANIZATION
DOI: 10.5281/zenodo.11530398
title: YUQORI DARAJALI TENGLAMALARNI YECHISH USULLARI.
DOI: 10.15468/dl.5a32zm
title: Occurrence Download

description: A dataset containing 12851 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bison bison (Linnaeus, 1758)",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 }
 ]
}

 The dataset includes 12851 records from 57 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0072293-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.11530397
title: YUQORI DARAJALI TENGLAMALARNI YECHISH USULLARI.
DOI: 10.15468/dl.sg3h6b
title: Occurrence Download

description: A dataset containing 147 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Leuresthes sardina (Jenkins & Evermann, 1889)"
 ]
}

 The dataset includes 147 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052252-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4m4yzc
title: Occurrence Download

description: A dataset containing 31661 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((-71.1628828000000055 42.4239342999999991, -71.1628828000000055 42.4258489799999978, -71.1628828000000055 42.4288899299999969, -71.1628828000000055 42.4313677499999997, -71.1628828000000055 42.4332824199999976, -71.1628828000000055 42.4351971000000034, -71.1608557299999944 42.4351971000000034, -71.1587160699999970 42.4351971000000034, -71.1552250299999969 42.4351971000000034, -71.1529727500000035 42.4351971000000034, -71.1504952399999979 42.4351971000000034, -71.1486933999999991 42.4351971000000034, -71.1486933999999991 42.4331698000000017, -71.1486933999999991 42.4304667199999983, -71.1486933999999991 42.4291151899999974, -71.1486933999999991 42.4274257699999993, -71.1486933999999991 42.4258489799999978, -71.1486933999999991 42.4239342999999991, -71.1513961499999965 42.4239342999999991, -71.1542115000000024 42.4239342999999991, -71.1562385600000056 42.4239342999999991, -71.1589412900000013 42.4239342999999991, -71.1602926599999961 42.4239342999999991, -71.1628828000000055 42.4239342999999991))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1932",
 "BasisOfRecord is one of (Human Observation, Living Specimen, Observation, Specimen, Machine Observation)"
 ]
}

 The dataset includes 31661 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0091926-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.cshbbg
title: Occurrence Download

description: A dataset containing 20109951 species occurrences available in GBIF matching the query:
{
 "Country" : [
 "is Colombia"
 ]
}

 The dataset includes 20109951 records from 3819 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052301-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.31120366
title: S1D Figure

description: S1D Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i+anti-IFN Abs samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORFK5 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 2 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.31119388.v1
title: S1A Figure

description: S1A Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORF50 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 1 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.31119520
title: S1B Figure

description: S1B Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i+anti-IFN Abs samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORF50 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 2 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.31119520.v1
title: S1B Figure

description: S1B Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i+anti-IFN Abs samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORF50 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 2 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.31120360
title: S1C Figure

description: S1C Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORFK5 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 1 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.31120360.v1
title: S1C Figure

description: S1C Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORFK5 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 1 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.31120366.v1
title: S1D Figure

description: S1D Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i+anti-IFN Abs samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORFK5 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 2 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.6084/m9.figshare.30577934
title: Figure 5H-I primary data

description: Figure 5H-I legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) to block IFN-β paracrine signaling. Protein was isolated from BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs based on tdTomato expression. Western blots were probed for total RelA and phosphorylated RelA (Ser536) and β-actin as a loading control. Levels of phosphorylated RelA (Ser536) were quantified by normalizing to total RelA and plotted relative to bulk lytic+casp-i+ anti-IFN Abs samples.
DOI: 10.6084/m9.figshare.30577934.v1
title: Figure 5H-I primary data

description: Figure 5H-I legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) to block IFN-β paracrine signaling. Protein was isolated from BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs based on tdTomato expression. Western blots were probed for total RelA and phosphorylated RelA (Ser536) and β-actin as a loading control. Levels of phosphorylated RelA (Ser536) were quantified by normalizing to total RelA and plotted relative to bulk lytic+casp-i+ anti-IFN Abs samples.
DOI: 10.6084/m9.figshare.31119388
title: S1A Figure

description: S1A Fig. Viral gene expression heterogeneity does not completely explain IFN-β expression. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Cells from the lytic+casp-i samples were collected both without sorting (“bulk”) and after sorting based on tdTomato expression (tdTomato + and -). RNA was extracted and KSHV ORF50 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. The fold enrichment of mRNA levels compared to unsorted (“bulk”) samples are plotted in log2 scale. Results in this figure were obtained from the same samples used in Fig 1 and are thus directly comparable to IFN-β enrichment shown in those Figure. ns = p&gt;0.05, ** = p&lt;0.01. One-Way ANOVA followed by Tukey’s post hoc multiple comparisons test. n≥ 4 for all experiments.
DOI: 10.15468/dl.sucjur
title: Occurrence Download

description: A dataset containing 3280 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Balanidae, Serpulidae, Watersiporidae, Styelidae, Pennariidae, Mytilidae, Teredinidae, Ulvaceae, Caulerpaceae, Bougainvilliidae, Syllidae, Hydractiniidae, Varunidae, Bolinopsidae)",
 "Geometry POLYGON ((33.925781 30.297018, 30.849609 30.221102, 36.738281 17.978733, 42.539063 11.867351, 46.054688 9.362353000000001, 53.613281 12.297068, 64.6875 21.616579, 66.269531 25.403585, 65.302734 29.993002, 50.976563 32.398516, 33.925781 30.297018))"
 ]
}

 The dataset includes 3280 records from 86 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010806-260129131611470/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.18476942
title: YIGIRISH KORXONALARIDAGI CHIQINDILAR TURLARI VA ULARNI QAYTA ISHLASHNING IQTISODIY SAMARADORLIGI

description: Ushbu maqolada tabiiy toladan chiqadigan chiqindilarni qayta ishlash va tabiiy toladan chiqindi chiqish miqdorini kamaytirish, xom ashyolardan oqilona foydalanish va ularni tejash masalalari ko‘rilgan. Yigirish korxonalarida hosil bo‘ladigan asosiy chiqindi turlari, ularning kelib chiqish sabablari hamda chiqindilarni qayta ishlash orqali iqtisodiy samaradorlikni oshirish masalalari yoritilgan. Shuningdek, ikkilamchi xomashyo sifatida chiqindilardan foydalanishning ishlab chiqarish tannarxini kamaytirish va resurs tejamkorligini ta’minlashdagi ahamiyati tahlil qilingan.
DOI: 10.5281/zenodo.18476941
title: YIGIRISH KORXONALARIDAGI CHIQINDILAR TURLARI VA ULARNI QAYTA ISHLASHNING IQTISODIY SAMARADORLIGI

description: Ushbu maqolada tabiiy toladan chiqadigan chiqindilarni qayta ishlash va tabiiy toladan chiqindi chiqish miqdorini kamaytirish, xom ashyolardan oqilona foydalanish va ularni tejash masalalari ko‘rilgan. Yigirish korxonalarida hosil bo‘ladigan asosiy chiqindi turlari, ularning kelib chiqish sabablari hamda chiqindilarni qayta ishlash orqali iqtisodiy samaradorlikni oshirish masalalari yoritilgan. Shuningdek, ikkilamchi xomashyo sifatida chiqindilardan foydalanishning ishlab chiqarish tannarxini kamaytirish va resurs tejamkorligini ta’minlashdagi ahamiyati tahlil qilingan.
DOI: 10.6084/m9.figshare.30577154
title: Figure 4H-J primary data

description: Figure 4H-J legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”). Where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Protein lysates were probed for phosphorylated IRF3 (Ser386), total IRF3, phosphorylated TBK1 (Ser172), total TBK1, and β-actin as a loading control. As a positive control for IRF3 and TBK1 activation/phosphorylation, A549 cells were treated with poly(I:C) for 6 and 48 hours before protein lysates were collected. Protein was isolated from treated BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs sample based on tdTomato expression.Western blot for phosphorylated TBK1 and phosphorylated IRF3 for bulk lytic+casp-i+anti-IFN Abs treated samples and sorted tdTomato+ and tdTomato- samples were quantified by normalizing the density of phosphorylated protein to unphosphorylated protein bands. Phosphorylation levels are plotted relative to the bulk lytic+casp-i or lytic+casp-i+anti-IFN Abs treated samples.
DOI: 10.6084/m9.figshare.30577154.v1
title: Figure 4H-J primary data

description: Figure 4H-J legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”). Where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. Protein lysates were probed for phosphorylated IRF3 (Ser386), total IRF3, phosphorylated TBK1 (Ser172), total TBK1, and β-actin as a loading control. As a positive control for IRF3 and TBK1 activation/phosphorylation, A549 cells were treated with poly(I:C) for 6 and 48 hours before protein lysates were collected. Protein was isolated from treated BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs sample based on tdTomato expression.Western blot for phosphorylated TBK1 and phosphorylated IRF3 for bulk lytic+casp-i+anti-IFN Abs treated samples and sorted tdTomato+ and tdTomato- samples were quantified by normalizing the density of phosphorylated protein to unphosphorylated protein bands. Phosphorylation levels are plotted relative to the bulk lytic+casp-i or lytic+casp-i+anti-IFN Abs treated samples.
DOI: 10.6084/m9.figshare.30577679
title: Figure 5D-F primary data

description: Figure 5D-F legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) to block IFN-β paracrine signaling. Protein was isolated from BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs based on tdTomato expression. Western blots were probed for ATF2, c-Jun, and β-actin as a loading control. Levels of ATF2 and c-Jun in sorted samples were quantified from the western blots, normalized to β-actin, and plotted relative to bulk lytic+casp-i+ anti-IFN Abs samples.