DOI: 10.5061/dryad.50kt0
title: Data from: Systems biology of tissue-specific response to Anaplasma phagocytophilum reveals differentiated apoptosis in the tick vector Ixodes scapularis

description: Anaplasma phagocytophilum is an emerging pathogen that causes human
 granulocytic anaplasmosis. Infection with this zoonotic pathogen affects
 cell function in both vertebrate host and the tick vector, Ixodes
 scapularis. Global tissue-specific response and apoptosis signaling
 pathways were characterized in I. scapularis nymphs and adult female
 midguts and salivary glands infected with A. phagocytophilum using a
 systems biology approach combining transcriptomics and proteomics.
 Apoptosis was selected for pathway-focused analysis due to its role in
 bacterial infection of tick cells. The results showed tissue-specific
 differences in tick response to infection and revealed differentiated
 regulation of apoptosis pathways. The impact of bacterial infection was
 more pronounced in tick nymphs and midguts than in salivary glands,
 probably reflecting bacterial developmental cycle. All apoptosis pathways
 described in other organisms were identified in I. scapularis, except for
 the absence of the Perforin ortholog. Functional characterization using
 RNA interference showed that Porin knockdown significantly increases tick
 colonization by A. phagocytophilum. Infection with A. phagocytophilum
 produced complex tissue-specific alterations in transcript and protein
 levels. In tick nymphs, the results suggested a possible effect of
 bacterial infection on the inhibition of tick immune response. In tick
 midguts, the results suggested that A. phagocytophilum infection inhibited
 cell apoptosis to facilitate and establish infection through up-regulation
 of the JAK/STAT pathway. Bacterial infection inhibited the intrinsic
 apoptosis pathway in tick salivary glands by down-regulating Porin
 expression that resulted in the inhibition of Cytochrome c release as the
 anti-apoptotic mechanism to facilitate bacterial infection. However, tick
 salivary glands may promote apoptosis to limit bacterial infection through
 induction of the extrinsic apoptosis pathway. These dynamic changes in
 response to A. phagocytophilum in I. scapularis tissue-specific
 transcriptome and proteome demonstrated the complexity of the tick
 response to infection and will contribute to characterize gene regulation
 in ticks.
description: File nymphs_replica
 1.raw.zipThis file contains the
 mass spectrometry data of iTRAQ labeled nymph sample (A.
 phagocytophilum-infected and uninfected control) replicate 1 (RAW.ZIP).
 Files may be viewed with Thermo Scientific Qual
 Browser.nymphs_replica
 1.raw.zipFile nymphs_replica
 2.raw.zipThis file contains the
 mass spectrometry data of iTRAQ labeled nymph sample (A.
 phagocytophilum-infected and uninfected control) replicate 2 (RAW.ZIP).
 Files may be viewed with Thermo Scientific Qual
 Browser.nymphs_replica
 2.raw.zipFile tissues_ replica
 1.raw.zipThis file contains the
 mass spectrometry data of iTRAQ labeled adult female gut and salivary
 gland sample (A. phagocytophilum-infected and uninfected control)
 replicate 1 (RAW.ZIP). Files may be viewed with Thermo Scientific Qual
 Browser.tissues_ replica
 1.raw.zipFile tissues_ replica
 2.raw.zipThis file contains the
 mass spectrometry data of iTRAQ labeled adult female gut and salivary
 gland sample (A. phagocytophilum-infected and uninfected control)
 replicate 2 (RAW.ZIP). Files may be viewed with Thermo Scientific Qual
 Browser.tissues_ replica
 2.raw.zipFolder RNAseq
 analysisThis folder contains
 RNAseq data analysis for A. phagocytophilum-infected and uninfected
 nymphs, guts and salivary glands samples replicates 1 and
 2.RNAseq
 analysis.zipFile
 Sample_Tissues_Ixo_all_samples.fastq.zipThis file contains the
 reads from I. scapularis adult female tissue samples replicates 1 and 2
 (FASTQ.ZIP).Sample_Tissues_Ixo_all_samples.fastq.zipFile Sample_Nymphs_Ixo_all_samples.fastq.zipThis file contains the reads from I. scapularis nymph samples replicates 1 and 2 (FASTQ.ZIP).Sample_Nymphs_Ixo_all_samples.fastq.zip