cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5066/p91hb8gi
title: Peak-flow frequency analyses for selected streamgages in Carbon County, Montana, based on data through water year 2018

description: The USGS Wyoming-Montana Water Science Center (WY-MT WSC) documented (Sando and McCarthy, 2018) methods for peak-flow frequency analysis following implementation of the Bulletin 17C guidelines. The methods are used to provide estimates of peak-flow quantiles for 50-, 42.9-, 20-, 10-, 4-, 2-, 1-, 0.5-, and 0.2-percent annual exceedance probabilities (AEPs) for selected streamgages operated by the WY-MT WSC. This data release presents peak-flow frequency analyses for selected streamgages in Carbon County, Montana, that were based on methods described by Sando and McCarthy (2018).
DOI: 10.21258/1441400
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897012
DOI: 10.21258/1471743
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897694
DOI: 10.21258/1458815
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 898815
DOI: 10.5281/zenodo.4311814
title: data

description: The original data for this data set can be accessed HERE. This data repository was created to facilitate the reproducibility of the work Learning function from structure in neuromorphic networks.
DOI: 10.21258/1471606
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 899186
DOI: 10.5281/zenodo.4269695
title: UKCA model output using the CRI-Strat mechanism

description: Processed UKCA model output data to accompany "The Common Representative Intermediates Mechanism version 2 in the United<br> Kingdom Chemistry and Aerosols Model" by Scott Archer-Nicholls et al., to be published in the Journal of Advances in Modeling Earth Systems (JAMES), submitted 2020. Preprint available: https://www.essoar.org/doi/10.1002/essoar.10505092.1 Data has monthly averages over the 9 years of model integration (2010-2018) for all prognostic and diagnostic variables used for analysis in the main paper. The model was run with four separate suites, corresponding to the four main scenarios evaluated in the main paper and summarised in Table 4 of the paper. The suite codes match with the scenarios as follows: * bt518: CRI-Strat, the Base CRI scenario * bt564: StratTrop, the Base stratTrop scenario * bt680: CRI_Emiss_ST, the CRI-Strat scenario with StratTrop emissions, used for isolating the effect of chemical representation without changes to emissions * bp480: CRI_Emiss_C2C3, the CRI-Strat scenario with speciated C2-C3 emissions, used for isolating the effect of NMVOC speciation without changing the total emitted mass. Please see the README in the package for more information.
description: {"references": ["https://www.essoar.org/doi/10.1002/essoar.10505092.1"]}
DOI: 10.21258/1448619
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897164
DOI: 10.5519/qd.hww2tent
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (2314 records)
DOI: 10.21258/1440528
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897782
DOI: 10.5519/qd.vcbzt1hv
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (2290 records)
DOI: 10.21258/1476997
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 893071
DOI: 10.21258/1448257
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 902251
DOI: 10.21258/1441015
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 899417
DOI: 10.21258/1458559
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 893020
DOI: 10.21258/1476073
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 891761
DOI: 10.5066/p9so2fvm
title: Seepage meter efficiency in highly permeable settings source data (2020)

description: Efficiency of seepage meters, long considered a fixed property associated with the meter design, is not constant in highly permeable sediments. Instead, results from this study indicate that efficiency varies substantially with seepage-bag fullness, duration of bag attachment, depth of meter insertion into the sediments, and seepage velocity. Efficiency also varies substantially in response to variable hydraulic conductivity. The first worksheet titled &quot;Literature efficiency&quot; presents seepage-meter efficiency values from the literature to show the progression of efficiency versus time. The remaining 7 worksheets present time-series data showing seepage-meter efficiency related to several different tests: (1) flow response to a series of bag attachments, (2) effect of bag fullness during upward flow at several seepage rates, (3) efficiency response during long bag attachments at a range of seepage rates, (4) efficiency during upward and downward seepage at a range of seepage rates, (5) efficiency in response to different insertion depths of the seepage cylinder, (6) efficiency in response to variable hydraulic conductivity, and (7) efficiency reduction during a prolonged seepage-meter installation.
DOI: 10.21258/1466169
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897401
DOI: 10.21258/1436282
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897992
DOI: 10.21258/1469976
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897646
DOI: 10.15468/wtukkj
title: The wild bees (Hymenoptera: Apoidea) of Morocco

description: This dataset contains the digitized treatments in Plazi based on the original journal article Lhomme, Patrick (2020): The wild bees (Hymenoptera: Apoidea) of Morocco. Zootaxa 4892 (1): 1-159, DOI: https://doi.org/10.11646/zootaxa.4892.1.1
DOI: 10.21258/1439332
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 898221
DOI: 10.21258/1454614
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 898225
DOI: 10.21258/1439381
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 898200
DOI: 10.5281/zenodo.4311513
title: hg38 Jannovar data files

description: Transcript files for Jannovar (PMID:24677618).
DOI: 10.21258/1465719
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 898719
DOI: 10.21258/1459503
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897203
DOI: 10.21258/1451899
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 898590
DOI: 10.21258/1441958
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897196
DOI: 10.21258/1471456
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897475
DOI: 10.21258/1459476
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897644
DOI: 10.5517/ccdc.csd.cc2460hw
title: CCDC 1971987: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460jx
title: CCDC 1971988: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460ky
title: CCDC 1971989: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460lz
title: CCDC 1971990: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460m0
title: CCDC 1971991: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460n1
title: CCDC 1971992: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460p2
title: CCDC 1971993: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460q3
title: CCDC 1971994: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460r4
title: CCDC 1971995: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460s5
title: CCDC 1971996: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460t6
title: CCDC 1971997: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460v7
title: CCDC 1971998: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460w8
title: CCDC 1971999: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460x9
title: CCDC 1972000: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460yb
title: CCDC 1972001: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2460zc
title: CCDC 1972002: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24610f
title: CCDC 1972003: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24611g
title: CCDC 1972004: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24612h
title: CCDC 1972005: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24613j
title: CCDC 1972006: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24614k
title: CCDC 1972007: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24615l
title: CCDC 1972008: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24616m
title: CCDC 1972009: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24617n
title: CCDC 1972010: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24618p
title: CCDC 1972011: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc24619q
title: CCDC 1972012: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2461br
title: CCDC 1972013: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc2461cs
title: CCDC 1972014: Experimental Crystal Structure Determination

description: Related Article: Matthew R. Gyton, Anthony R. Leverett, Marcus L. Cole, Alasdair I. McKay|2020|Dalton Trans.|49|5653|doi:10.1039/D0DT00285B
DOI: 10.5517/ccdc.csd.cc25v28d
title: CCDC 2021053: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v29f
title: CCDC 2021054: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2bg
title: CCDC 2021055: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2ch
title: CCDC 2021056: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2dj
title: CCDC 2021057: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2fk
title: CCDC 2021058: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.21258/1452514
title: Schweizerisches Landesforstinventar - Ergebnistabelle Nr. 897601
DOI: 10.5517/ccdc.csd.cc25v2gl
title: CCDC 2021059: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2hm
title: CCDC 2021060: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2jn
title: CCDC 2021061: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2kp
title: CCDC 2021062: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5517/ccdc.csd.cc25v2lq
title: CCDC 2021063: Experimental Crystal Structure Determination

description: Related Article: Ingo Koehne, Artur Lik, Miriam Gerstel, Clemens Bruhn, Johann Peter Reithmaier, Mohamed Benyoucef, Rudolf Pietschnig|2020|Dalton Trans.|49|16683|doi:10.1039/D0DT03047C
DOI: 10.5281/zenodo.2646539
title: Homologous membrane protein structures (HOMEP) dataset version v2

description: <strong>Protein structures from the dataset of Homologous MEmbrane Protein structures (HOMEP)</strong> version v2 created in 2010, published in 2013. A more automated version of HOMEP v1: 10.5281/zenodo.2646534<br>
 

<strong>Table 1</strong> = List of protein databank structure entries<br>
From Stamm et al, PLOS One 2013, https://www.ncbi.nlm.nih.gov/pubmed/23469223, Supplementary Table 1, with the following entries:<br>
Family grouping, Protein databank identifier, Name, Source organism, Resolution (Å)

 

<strong>Table 2</strong> = List of pairs of structures<br>
From Stamm et al, PLOS One 2013, https://www.ncbi.nlm.nih.gov/pubmed/23469223, Supplementary Table 2, with the following entries:<br>
Family grouping, PDB code for first structure, Chain ID from PDB1, PDB for second structure, Chain ID from PDB2, protein structural difference (PSD), % sequence identity

 

<strong>File S2 HOMEP2 Dataset.tar.gz</strong> = Protein databank format files (PDB) are attached in the Dataset tar zipped file, organized by family. From Stamm et al, PLOS One 2013, https://www.ncbi.nlm.nih.gov/pubmed/23469223, Supplementary dataset.
description: Funded by the Max Planck Gesellschaft and the German Research Foundation (DFG; www.dfg.de/en) Collaborative Research Center 807 "Transport and Communication across Biological Membranes".
description: {"references": ["Stamm M, Staritzbichler R, Khafizov K, Forrest LR. Alignment of helical membrane protein sequences using AlignMe. PLoS One. 2013;8(3):e57731. doi: 10.1371/journal.pone.0057731. Epub 2013 Mar 4. PubMed PMID: 23469223; PubMed Central PMCID: PMC3587630"]}
DOI: 10.25407/jacbts.8253722.v1
title: Figure 1: Dynamics of GPVI-Fc Binding and Platelet Adhesion to Collagen Under Flow

description: Glycoprotein VI–fragment crystallizable (GPVI-Fc) (50 μg/ml final concentration; 333 nM) labeled with phycoerythrin (PE)-conjugated anti–human-Fc antibody <b>(red)</b> was added to blood containing abciximab (to inhibit platelet aggregation and allow only platelet adhesion) before perfusion over collagen (550/s). Differential interference contrast (DIC) and fluorescence images were taken by video microscopy (1 frame/2 s) using a 100× NA1.4 oil objective. Images are representative for 6 individual experiments. <b>Upper rows:</b> overlay of DIC (collagen/platelets) and fluorescence (PE-labeled GPVI-Fc) images. <b>Bottom rows:</b> fluorescence images of PE-labeled GPVI-Fc. See also Video 1. <b>(A)</b> A single platelet <b>(thick arrow)</b> rolls over PE-labeled GPVI-Fc <b>(thin arrows in row below)</b> bound to collagen. Two platelets <b>(asterisks)</b> adhere to segments of collagen fibers that contain less fluorescent GPVI-Fc. <b>(B)</b> A single platelet <b>(arrow, top row)</b> attaching downstream of the fiber and moving back against the blood flow displaces GPVI-Fc-PE from collagen <b>(arrows in bottom row)</b>. <b>(C)</b> Structured illumination microscopy imaging. Platelets adhere to collagen segments carrying little GPVI-Fc. Collagen coated onto glass coverslips was stained with anti–collagen type I and type III antibody (Ab) and AlexaFluor405-conjugated secondary Ab <b>(blue)</b>. PE-labeled GPVI-Fc (50 μg/ml) was added to blood containing Arg-Gly-Asp-Ser (to inhibit platelet aggregation and allow only platelet adhesion) before the start of perfusion (shear rate 550/s). After 4 min of flow, platelets were fixed and stained with anti-CD41 Ab and DyLight 488-conjugated secondary Ab <b>(green)</b>. The fluorescence micrograph shows a maximum intensity projection of 0.15 μm z sections (total z 2.5 μm) of a structured illumination microscopy image (ELYRA PS.1; Carl Zeiss MicroImaging GmbH, Jena, Germany). <b>i</b> and <b>ii</b>, Identical pictures. <b>i,</b> Binding of GPVI-Fc <b>(red)</b> onto collagen fibers <b>(blue)</b>. Green channel (platelets) was omitted. <b>ii,</b> Picture as in <b>i</b> but with platelets <b>(green)</b>. Image is representative of 5 others from different experiments. Video 2 presents a 3-dimensional representation.
DOI: 10.25407/jacbts.8253722
title: Figure 1: Dynamics of GPVI-Fc Binding and Platelet Adhesion to Collagen Under Flow

description: Glycoprotein VI–fragment crystallizable (GPVI-Fc) (50 μg/ml final concentration; 333 nM) labeled with phycoerythrin (PE)-conjugated anti–human-Fc antibody <b>(red)</b> was added to blood containing abciximab (to inhibit platelet aggregation and allow only platelet adhesion) before perfusion over collagen (550/s). Differential interference contrast (DIC) and fluorescence images were taken by video microscopy (1 frame/2 s) using a 100× NA1.4 oil objective. Images are representative for 6 individual experiments. <b>Upper rows:</b> overlay of DIC (collagen/platelets) and fluorescence (PE-labeled GPVI-Fc) images. <b>Bottom rows:</b> fluorescence images of PE-labeled GPVI-Fc. See also Video 1. <b>(A)</b> A single platelet <b>(thick arrow)</b> rolls over PE-labeled GPVI-Fc <b>(thin arrows in row below)</b> bound to collagen. Two platelets <b>(asterisks)</b> adhere to segments of collagen fibers that contain less fluorescent GPVI-Fc. <b>(B)</b> A single platelet <b>(arrow, top row)</b> attaching downstream of the fiber and moving back against the blood flow displaces GPVI-Fc-PE from collagen <b>(arrows in bottom row)</b>. <b>(C)</b> Structured illumination microscopy imaging. Platelets adhere to collagen segments carrying little GPVI-Fc. Collagen coated onto glass coverslips was stained with anti–collagen type I and type III antibody (Ab) and AlexaFluor405-conjugated secondary Ab <b>(blue)</b>. PE-labeled GPVI-Fc (50 μg/ml) was added to blood containing Arg-Gly-Asp-Ser (to inhibit platelet aggregation and allow only platelet adhesion) before the start of perfusion (shear rate 550/s). After 4 min of flow, platelets were fixed and stained with anti-CD41 Ab and DyLight 488-conjugated secondary Ab <b>(green)</b>. The fluorescence micrograph shows a maximum intensity projection of 0.15 μm z sections (total z 2.5 μm) of a structured illumination microscopy image (ELYRA PS.1; Carl Zeiss MicroImaging GmbH, Jena, Germany). <b>i</b> and <b>ii</b>, Identical pictures. <b>i,</b> Binding of GPVI-Fc <b>(red)</b> onto collagen fibers <b>(blue)</b>. Green channel (platelets) was omitted. <b>ii,</b> Picture as in <b>i</b> but with platelets <b>(green)</b>. Image is representative of 5 others from different experiments. Video 2 presents a 3-dimensional representation.