DOI: 10.5061/dryad.1g1jwsv4s
title: Data from: Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict

description: Spatial structure is a common feature of all naturally occurring
 populations. Theoretically, spatial structuring of a habitat could
 modulate the intensity of interlocus sexual conflict (ISC) in a
 population, possibly by modulating intersexual encounter rate. We tested
 this theory using laboratory populations of Drosophila melanogaster by
 measuring male-induced decline in female fitness in three-patch habitat
 systems with two alternative habitat types: structured–interconnected and
 unstructured. Our results on reproductive and survival costs in females
 suggested significant costs attributable to male presence (i.e. ISC) and
 to living on structured habitat. However, there was only weak evidence
 supporting the theory of modulation of ISC by habitat structuring only.
 Through a follow-up experiment, we also showed that the effect of habitat
 on ISC is robust to the alteration of female conditions. Therefore, it
 appears that spatial structuring per se is unlikely to modulate ISC but
 can impose additional survival costs. We also showed that this survival
 cost could be sex specific, possibly reflecting female-biased spontaneous
 dispersal.
description: Two randomized block design experiments were conducted on
 laboratory adapted populations of <em>Drosophila
 melanogaster</em>. The details of these populations were reported
 first in Nandy et al. 2016 (https://doi.org/10.1016/j.anbehav.2016.01.019). Laboratory structured habitat was set up by using interconnected plastic vials (for three-patch setups) or one vial and one bottle (for two-patch setups). All traits reported in this dataset were measured in the laboratory - close to the populations' usual natural holding condition (i.e., the laboratory conditions in which they are usually maintained). For full experimental details, see the pre-print - https://www.biorxiv.org/content/10.1101/2022.10.29.514349v3. 
description: # Data from: Spatial structure imposes sex-specific costs but does not
 reduce interlocus sexual conflict **Key to reading the data file:** Data
 from all the experiments reported in the manuscript titled “Spatial
 structure imposes sex-specific costs but does not reduce interlocus sexual
 conflict” can be found in the spreadsheet file. Data of different assays
 from all the experiments have been separated into different tabs in the
 spreadsheet. Data are arranged vertically with factors/treatments
 separated in columns. Columns are clearly labeled. The details of the tabs
 are following. Tab - Ex1_Progeny count (**Tab 1**): Data on progeny output
 (MPO/Mean Progeny Output) under two types of Male exposures – limited
 exposure (LE), continuous exposure (CE), and two levels of Habitat i.e.,
 Structured and Unstructured is from Experiment 1(Ex 1). ‘BLOCK’ refers to
 three randomized blocks mentioned as B_2, B_3, B_5. Count data is unit
 less. Tab - Ex1_Starvation survival time (**Tab 2**): Data on starvation
 survival time (MST/ Mean Starvation survival time) under two types of Male
 exposures – limited exposure (LE), continuous exposure (CE) and two levels
 of Habitat i.e., Structured and Unstructured is from Experiment 1(Ex 1).
 ‘BLOCK’ refers to three randomized block mentioned as B_2, B_3, B_5. Unit
 of starvation survival time measure is in hours Tab - Dietary treatment
 and dispersal (**Tab 3**): ‘Treatment’ refers to the levels of dietary
 manipulation (Control, 20%, 40% and 60%). ‘Sex’ refers to Male (M) and
 Female (F). ‘BLOCK’ refers to three randomized block mentioned as B_4,
 B_2, B_3.  ‘Trial’ refers to total number of one particular sex i.e., 15
 and ‘Dispersal’ refers to the absolute number of flies that dispersed. Tab
 - Ex2_Progeny count (**Tab 4**): Data on progeny output (MPO/Mean Progeny
 Output) for two types of ‘Female’- Poor (i.e., larval development in 40%
 diluted food) and Standard under two types of Male exposures – limited
 exposure (LE), continuous exposure (CE) and two levels of Habitat i.e.,
 Structured and Unstructured is from Experiment 2(Ex 2). ‘BLOCK’ refers to
 three randomized block mentioned as BL_1, BL_2, BL_3. Count data is unit
 less. Tab - Ex2_Starvation survival time (**Tab 5**): Data on starvation
 survival time (MST/ Mean Starvation survival time) for two types of
 ‘Female’- Poor and Standard under two types of under two types of Male
 exposures – limited exposure (LE), continuous exposure (CE) and two levels
 of Habitat i.e., Structured and Unstructured is from Experiment 2(Ex 2).
 ‘BLOCK’ refers to three randomized block mentioned as BL_1, BL_2, BL_3.
 Unit of starvation survival time measure is in hours. Tab - Ex2_MST_Male
 (**Tab 6**): Data on starvation survival time (MST/ Mean Starvation
 survival time) for two types of ‘Female’- Poor and Standard and two levels
 of Habitat i.e., Structured and Unstructured is from Experiment 2(Ex 2).
 ‘BLOCK’ refers to three randomized block mentioned as BL_1, BL_2, BL_3. 
 Unit of starvation survival time measure is in hours. Tab - Distribution
 in 3 patch system (**Tab 7**): Proportion of Female: ‘Time’ refers to each
 time point (1H, 2H, 3H,5H,6H,12H,18H,24H,30H,36H,42H,48H) counting was
 done. P1SR, P2SR, P3SR refers to Sex Ratio (SR) from Patch 1, 2 and 3
 respectively. Here, sex ratio indicates proportion of females. Total
 number of flies:P1O, P2O, P3O refers to observed number of flies in patch
 1, 2, 3 respectively. P1E, P2E, P3E refers to expected number of flies in
 patch 1, 2, 3 respectively.  Number of flies in corridor:No’ refers to
 absolute count of flies, ‘Sex’ refers to Male and Female. Tab - Sex
 specific dispersal (**Tab 8**): ‘Sex’ refers to Male (M) and Female (F).
 ‘Time’ refers to each time point i.e., 2H, 4H and 6H when dispersal was
 counted ‘Trial’ refers to total number of one particular sex present in
 the source patch and ‘Dispersal’ refers to the absolute number of flies
 that dispersed. Tab - Relative Reduction (**Tab 9**): ‘Female’ refers Poor
 (i.e., larval development in 40% diluted food) and Standard. ‘LE’ refers
 to progeny output at limited exposure. ‘CE’ refers to progeny output at
 continuous exposure. ‘Habitat’ refers to two levels of habitat type-
 Structured and Unstructured. ‘RR’ refers to Relative Reduction. ‘Block’
 refers to three randomized block -1, 2 and 3.