cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/89xj9-cwf77
title: Adipocyte Cell

description: FGF21Dose-symmetrical dose scheme
description: <strong>Tilt Series Date:</strong> 2017-11-30</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Primary adipocyte cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 50000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Roger Kornberg and Maia</p>
<strong>Purification / Growth Conditions / Treatment:</strong> https://elifesciences.org/articles/43146#s4</p>
<strong>Sample Preparation:</strong> https://elifesciences.org/articles/43146#s4</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-23/rawdata/</p>tilt_series_12_sorted.mrc,  Tilt Series (Pixel Size 2.241 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-23/rawdata/tilt_series_12_sorted.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_12_sorted_part61_4.rec,  Reconstruction (Pixel Size 8.964 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-23/3dimage_117266/tilt_series_12_sorted_part61_4.rec"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_1_sorted_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-23/3dimage_118712/tilt_series_1_sorted_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f1scv-zeh28
title: hns_overexpression_PS152

description: Andrew's DNA project
description: <strong>Tilt Series Date:</strong> 2016-08-24</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown for 27h from glycerol stock. At OD 0.51 induced with ~3mM IPTG and grown for 4h and froze as such, no spinning. OD was 0.52. Cell density and distribution looked good.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-46/rawdata/</p>hnsM9_PS152_atlas10004.mrc,  Tilt Series (Pixel Size 0.6 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-46/rawdata/hnsM9_PS152_atlas10004.mrc"
        > <i class="download icon"></i> Download </a></p>

        hnsM9_PS152_atlas10004_part81_2.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-46/3dimage_78545/hnsM9_PS152_atlas10004_part81_2.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2016-08-24-46_slicer17030.png,  706.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-46_slicer17030.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7acea5
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Zosterops splendidus",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044016-250811113504898/datasets/export for details.
DOI: 10.15468/dl.cwxqy3
title: Occurrence Download

description: A dataset containing 76 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Linaria vulgaris",
 "Country is Bulgaria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 76 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044017-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/02rqk-c2z76
title: Adipocyte Cell

description: FGF21Dose-symmetrical dose scheme
description: <strong>Tilt Series Date:</strong> 2017-11-30</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Primary adipocyte cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 50000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Roger Kornberg and Maia</p>
<strong>Purification / Growth Conditions / Treatment:</strong> https://elifesciences.org/articles/43146#s4</p>
<strong>Sample Preparation:</strong> https://elifesciences.org/articles/43146#s4</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-21/rawdata/</p>tilt_series_9_sorted.mrc,  Tilt Series (Pixel Size 2.241 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-21/rawdata/tilt_series_9_sorted.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_9_sorted.rec,  Reconstruction (Pixel Size 8.964 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-21/3dimage_117263/tilt_series_9_sorted.rec"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_1_sorted_orig_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-21/3dimage_118722/tilt_series_1_sorted_orig_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8z7n6-8jk20
title: Crosslinked HIV-1 cores TRIM5α

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5α
description: <strong>Tilt Series Date:</strong> 2015-01-03</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 84.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> the TRIM5α-core complexes will be purified through a sucrose cushion to remove excess unbound TRIM5alpha before crosslinking and freezing</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores decorated with TRIM5α. 0.3 µM sulfo-EGS</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-52/3dimage_73527/</p>tomography0008_full.rec,  Reconstruction (Pixel Size 0.0 nm), 355.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-52/3dimage_73527/tomography0008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography0008.rec,  Reconstruction (Pixel Size 0.0 nm), 346.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-52/3dimage_73526/tomography0008.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography0008.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-52/rawdata/tomography0008.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.5scerk
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Linaria vulgaris",
 "Country is Chile",
 "HasCoordinate is true"
 ]
}

 The dataset includes 3 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044019-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/sgjjz-m3373
title: Cell line25 incubated MG-132

description: 4 hours incubation MG-132 (0.4 ul) 1 μg/ml. Added 500 ul of Vpr-tomato pseudovirus to each well.
description: <strong>Tilt Series Date:</strong> 2016-02-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown on R2/2 London finder grids coated with fibronectin</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-5/rawdata/</p>tomography5.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-5/rawdata/tomography5.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography5_part121_24.rec,  Reconstruction (Pixel Size 2.4 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-5/3dimage_91257/tomography5_part121_24.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography5_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-5/3dimage_91462/tomography5_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.dp3wzb
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Melanotaenia ogilbyi)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044015-250811113504898/datasets/export for details.
DOI: 10.22002/m8gmm-0w422
title: HeLa cells expressing TRIM5α-YFP

description: 4 hours incubation MG-132 (0.4 ul) 1 μg/ml.
description: <strong>Tilt Series Date:</strong> 2016-05-05</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown on R2/2 London finder grids coated with fibronectin</p>
<strong>Sample Preparation:</strong> Manual Blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-05-05-9/rawdata/</p>tomography9.mrc,  Tilt Series (Pixel Size 6.167 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-05-05-9/rawdata/tomography9.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography9.rec,  Reconstruction (Pixel Size 24.668 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-05-05-9/3dimage_118365/tomography9.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography9secondjoinnewnew_full.rec,  Reconstruction (Pixel Size 0.0 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-05-05-9/3dimage_118478/tomography9secondjoinnewnew_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jjyr9-60a82
title: CLEM of TRIM5a-YFP in HeLa cells

description: HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads 1/300. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS
description: <strong>Tilt Series Date:</strong> 2015-01-21</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 234.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-5/rawdata/</p>tomgraphytest4.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-5/rawdata/tomgraphytest4.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest4.rec,  Reconstruction (Pixel Size 2.4 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-5/3dimage_51197/tomgraphytest4.rec"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest4_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-5/3dimage_56132/tomgraphytest4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7qxtxd
title: Occurrence Download

description: A dataset containing 7856 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Linaria vulgaris",
 "Country is Canada",
 "HasCoordinate is true"
 ]
}

 The dataset includes 7856 records from 41 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044018-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/agaqb-w3856
title: Adipocyte Cell

description: FGF21Dose-symmetrical dose scheme
description: <strong>Tilt Series Date:</strong> 2017-11-30</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Primary adipocyte cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 50000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Roger Kornberg and Maia</p>
<strong>Purification / Growth Conditions / Treatment:</strong> https://elifesciences.org/articles/43146#s4</p>
<strong>Sample Preparation:</strong> https://elifesciences.org/articles/43146#s4</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-22/rawdata/</p>tilt_series_11_sorted.mrc,  Tilt Series (Pixel Size 2.241 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-22/rawdata/tilt_series_11_sorted.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_11_sorted_part61_9.rec,  Reconstruction (Pixel Size 8.964 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-22/3dimage_117262/tilt_series_11_sorted_part61_9.rec"
        > <i class="download icon"></i> Download </a></p>

        tilt_series_3_sortednew_full.rec,  Reconstruction (Pixel Size 0.0 nm), 977.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2017-11-30-22/3dimage_118711/tilt_series_3_sortednew_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bnd7b-jb097
title: hns_overexpression_PS152

description: Andrew's DNA project
description: <strong>Tilt Series Date:</strong> 2016-08-24</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown for 27h from glycerol stock. At OD 0.51 induced with ~3mM IPTG and grown for 4h and froze as such, no spinning. OD was 0.52. Cell density and distribution looked good.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-50/rawdata/</p>hnsM9_PS152_atlas10008.mrc,  Tilt Series (Pixel Size 0.6 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-50/rawdata/hnsM9_PS152_atlas10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        hnsM9_PS152_atlas10008_part81_37.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-50/3dimage_78553/hnsM9_PS152_atlas10008_part81_37.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2016-08-24-50_slicer17032.png,  filaments with approximately 10nm spacing (10.154nm) -andrew, 518.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-50_slicer17032.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4yf06-1hh97
title: Cell line25 incubated MG-132

description: 4 hours incubation MG-132 (0.4 ul) 1 μg/ml. Added 500 ul of Vpr-tomato pseudovirus to each well.
description: <strong>Tilt Series Date:</strong> 2016-02-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown on R2/2 London finder grids coated with fibronectin</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-2/rawdata/</p>tomography2.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-2/rawdata/tomography2.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography2_part121_3.rec,  Reconstruction (Pixel Size 2.4 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-2/3dimage_91252/tomography2_part121_3.rec"
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        tomography2_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
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