cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7508960.v1
title: Effects of Isaria (Persoon) isolates on the subterranean termite Coptotermes gestroi (Wasmann) (Isoptera: Rhinotermitidae)

description: Coptotermes gestroi (Wasmann) is considered to be one of the most destructive species of subterranean termites because of the damage caused on edifications, urban trees, and crops. One alternative to control it is the use of biocontrol agents, such as the entomopathogenic fungi. The genus Isaria (Persoon) has been suggested for the control of subterranean termites, including the Coptotermes genus. Therefore, the present work aim was to select isolates of Isaria pathogenic to C. gestroi. The workers were sprayed with fungi suspensions of I. farinosa, I. fumosoroseaand I. javanica, and evaluated every day to determine mortality. All isolates were pathogenic towards C. gestroi with mortality rates higher than 70%, and virulent, with mean of survival of 2.0 up to 3.9 days. Among the isolates, the ESALQ-1205 isolate of I. farinosa, the ESALQ-1296 isolate of I. fumosorosea, URM-4995 and URM-4993 isolates of I. javanica showed the highest virulence. The estimated CL50 for the ESALQ-1205 isolate of I. farinosa, URM-4995 isolate of I. javanica and ESALQ-1296 isolate of I. fumosorosea was 3.7 x 105, 1.4 x 106and 2.7 x 106 conidia mL-1, respectively. These results suggest high efficiency of these isolates towards the C. gestroi workers. Nevertheless, further studies are necessary to determine a way of using them as well as their efficacy in field.
DOI: 10.6084/m9.figshare.7508960
title: Effects of Isaria (Persoon) isolates on the subterranean termite Coptotermes gestroi (Wasmann) (Isoptera: Rhinotermitidae)

description: Coptotermes gestroi (Wasmann) is considered to be one of the most destructive species of subterranean termites because of the damage caused on edifications, urban trees, and crops. One alternative to control it is the use of biocontrol agents, such as the entomopathogenic fungi. The genus Isaria (Persoon) has been suggested for the control of subterranean termites, including the Coptotermes genus. Therefore, the present work aim was to select isolates of Isaria pathogenic to C. gestroi. The workers were sprayed with fungi suspensions of I. farinosa, I. fumosoroseaand I. javanica, and evaluated every day to determine mortality. All isolates were pathogenic towards C. gestroi with mortality rates higher than 70%, and virulent, with mean of survival of 2.0 up to 3.9 days. Among the isolates, the ESALQ-1205 isolate of I. farinosa, the ESALQ-1296 isolate of I. fumosorosea, URM-4995 and URM-4993 isolates of I. javanica showed the highest virulence. The estimated CL50 for the ESALQ-1205 isolate of I. farinosa, URM-4995 isolate of I. javanica and ESALQ-1296 isolate of I. fumosorosea was 3.7 x 105, 1.4 x 106and 2.7 x 106 conidia mL-1, respectively. These results suggest high efficiency of these isolates towards the C. gestroi workers. Nevertheless, further studies are necessary to determine a way of using them as well as their efficacy in field.
DOI: 10.6084/m9.figshare.14327102
title: Climatic drivers of Sphagnum species distributions environmental layers, presences and output rasters

description: Output rasters, presences and climate rasters at all resolutions from Climatic drivers of <i>Sphagnum </i>species distributions
DOI: 10.6084/m9.figshare.7517573
title: Bioecological and behavioral genetic aspects involved in the conservation of the stingless bee Melipona subnitida Ducke (Apidae, Meliponini) and the use of molecular tools in studies of diversity

description: The stingless bee Melipona subnitida is now present almost everywhere in the Brazilian Northeastern, as a consequence of its good adaptability to the semiarid and economic and ecological potential offered by the honey production and pollination of commercial crops under confined conditions. Nevertheless, it is an endangered species due to environmental degradation processes, among which are: deforestation, indiscriminate use of pesticides and honey extraction. Such interference tends to isolate populations of Jandaíra causing a decrease in genetic variability, and therefore a reduction in the adaptive capacity of the species. However, advanced Molecular Biology techniques have been used allowing such populations to be assessed for their degree of genetic variability. Molecular markers such as microsatellite DNA are widely applied to genetic diversity studies. However, due to the high costs required for their development, several studies have been focused on the use of microsatellites transferred from closely related taxa with much success in studies on the genetic characterization of species and their populations. Therefore, this review aimed to evaluate the most relevant ecological and behavioral aspects in order to assist the population genetic studies of the stingless bee M. subnitida.
DOI: 10.6084/m9.figshare.7517573.v1
title: Bioecological and behavioral genetic aspects involved in the conservation of the stingless bee Melipona subnitida Ducke (Apidae, Meliponini) and the use of molecular tools in studies of diversity

description: The stingless bee Melipona subnitida is now present almost everywhere in the Brazilian Northeastern, as a consequence of its good adaptability to the semiarid and economic and ecological potential offered by the honey production and pollination of commercial crops under confined conditions. Nevertheless, it is an endangered species due to environmental degradation processes, among which are: deforestation, indiscriminate use of pesticides and honey extraction. Such interference tends to isolate populations of Jandaíra causing a decrease in genetic variability, and therefore a reduction in the adaptive capacity of the species. However, advanced Molecular Biology techniques have been used allowing such populations to be assessed for their degree of genetic variability. Molecular markers such as microsatellite DNA are widely applied to genetic diversity studies. However, due to the high costs required for their development, several studies have been focused on the use of microsatellites transferred from closely related taxa with much success in studies on the genetic characterization of species and their populations. Therefore, this review aimed to evaluate the most relevant ecological and behavioral aspects in order to assist the population genetic studies of the stingless bee M. subnitida.
DOI: 10.6084/m9.figshare.7510694
title: Technical feasibility of the QuickStix kit™ in identifying Phakopsora pachyrhizi captured by the spores collector

description: The aim of this study was to test the efficiency of QuickStix™ kit use for identification of Phakopsora pachyrhizi spores, which are the causal agents of Asian soybean rust, collected by "SIGA" spores collector. This kit is capable of detecting the presence of Phakopsora pachyrhizi in the early stages of leaf infection by determining the presence or absence of pathogen in the sample. It captures propagules of fungi present in the air that are identified or quantified by scanning blades in ordinary optical microscope. Different numbers of spores were tested with 4, 8 and 12 drops of extraction solution. For positive results, intensity of the test line and time for its occurrence were evaluated. The kit was able to detect small amounts of spores and the proportion of positive results was directly proportional to the number of spores and inversely proportional to the amount of drops. The proper portion of drops of extraction solution was four, as it showed more positive results for all classes of numbers of spores. No difference was found between the time to occurrence of positive results for the different treatments. The intensity of the test line was higher for classes with larger numbers of spores.
DOI: 10.6084/m9.figshare.7510694.v1
title: Technical feasibility of the QuickStix kit™ in identifying Phakopsora pachyrhizi captured by the spores collector

description: The aim of this study was to test the efficiency of QuickStix™ kit use for identification of Phakopsora pachyrhizi spores, which are the causal agents of Asian soybean rust, collected by "SIGA" spores collector. This kit is capable of detecting the presence of Phakopsora pachyrhizi in the early stages of leaf infection by determining the presence or absence of pathogen in the sample. It captures propagules of fungi present in the air that are identified or quantified by scanning blades in ordinary optical microscope. Different numbers of spores were tested with 4, 8 and 12 drops of extraction solution. For positive results, intensity of the test line and time for its occurrence were evaluated. The kit was able to detect small amounts of spores and the proportion of positive results was directly proportional to the number of spores and inversely proportional to the amount of drops. The proper portion of drops of extraction solution was four, as it showed more positive results for all classes of numbers of spores. No difference was found between the time to occurrence of positive results for the different treatments. The intensity of the test line was higher for classes with larger numbers of spores.
DOI: 10.6084/m9.figshare.7508141
title: Occurrence of Cryptosporidium spp. in calves buffaloes in the state of Pará

description: Cryptosporidiosis is a zoonosis caused by opportunistic protozoa of the genus Cryptosporidium spp. that attacks various animal species, including humans. This study had the objective of determining the occurrence of infection by Cryptosporidium spp. in buffalo raised in the state of Pará, Brazil, and establishing the relations between Cryptosporidium spp., the age of the animal and the presence of clinical signs of diarrhea. A total of 253 samples of feces from one day to 6-month old buffalo were analyzed. The fecal samples, collected directly from the rectum, were classified as diarrheal and non-diarrheal and analyzed with an optical microscope after centrifuge-concentration and staining with Kinyoun stain. Laboratory analysis revealed the presence of Cryptosporidium spp. in 2.37% (6/253) samples, all of which had a firm consistency. No significant relation was established between infection by Cryptosporidium spp., the animals age from one day to 6-month and clinical symptoms of diarrhea.
DOI: 10.6084/m9.figshare.7508141.v1
title: Occurrence of Cryptosporidium spp. in calves buffaloes in the state of Pará

description: Cryptosporidiosis is a zoonosis caused by opportunistic protozoa of the genus Cryptosporidium spp. that attacks various animal species, including humans. This study had the objective of determining the occurrence of infection by Cryptosporidium spp. in buffalo raised in the state of Pará, Brazil, and establishing the relations between Cryptosporidium spp., the age of the animal and the presence of clinical signs of diarrhea. A total of 253 samples of feces from one day to 6-month old buffalo were analyzed. The fecal samples, collected directly from the rectum, were classified as diarrheal and non-diarrheal and analyzed with an optical microscope after centrifuge-concentration and staining with Kinyoun stain. Laboratory analysis revealed the presence of Cryptosporidium spp. in 2.37% (6/253) samples, all of which had a firm consistency. No significant relation was established between infection by Cryptosporidium spp., the animals age from one day to 6-month and clinical symptoms of diarrhea.
DOI: 10.6084/m9.figshare.6992771
title: Occurrence and antimicrobial resistance profile of Salmonella spp. serotypes isolated from poultry farms in Paraná, Brazil

description: Te present study was carried to verify the resistance profile of different Salmonella spp. serotypes isolated from a poultry broiler house against antimicrobial agents. Tree-hundred and forty two drag swabs from poultry farms in western Paraná were processed in the period from January 2010 to January 2011, and 39 Salmonella spp. Strains were isolated. The serovars were mostly: S. Heidelberg, S. Mbandaka, S. Newport, S. Schwarzengrund, S. Enteritidis, S. Livingstone, S. Orion, S. Give and S. Infantis. The determination of the resistance profile to the 19 identified Salmonella serotypes was evaluated against 12 antimicrobial commercials. The results indicate that 51% of Salmonella serotypes showed resistance to on or more antimicrobials, with 12 different resistance patterns. The highest percentage of resistance was related to tetracycline (30,8%) and the lowest one to gentamicin and chloramphenicol (2,6%). The resistance levels indicate that antimicrobials should be used on poultry farms more carefuly, thus seeking to minimize the spread of resistant strains.
DOI: 10.6084/m9.figshare.6992771.v1
title: Occurrence and antimicrobial resistance profile of Salmonella spp. serotypes isolated from poultry farms in Paraná, Brazil

description: Te present study was carried to verify the resistance profile of different Salmonella spp. serotypes isolated from a poultry broiler house against antimicrobial agents. Tree-hundred and forty two drag swabs from poultry farms in western Paraná were processed in the period from January 2010 to January 2011, and 39 Salmonella spp. Strains were isolated. The serovars were mostly: S. Heidelberg, S. Mbandaka, S. Newport, S. Schwarzengrund, S. Enteritidis, S. Livingstone, S. Orion, S. Give and S. Infantis. The determination of the resistance profile to the 19 identified Salmonella serotypes was evaluated against 12 antimicrobial commercials. The results indicate that 51% of Salmonella serotypes showed resistance to on or more antimicrobials, with 12 different resistance patterns. The highest percentage of resistance was related to tetracycline (30,8%) and the lowest one to gentamicin and chloramphenicol (2,6%). The resistance levels indicate that antimicrobials should be used on poultry farms more carefuly, thus seeking to minimize the spread of resistant strains.
DOI: 10.25345/c5zf8q
title: MassIVE MSV000087112 - Baylor-vaccine-mice-heart-tissue-positive-RA-03-17-2021
title: baylor vaccine project, mice heart tissue RA, LC MS run data, 03 17 2021
DOI: 10.6084/m9.figshare.6992780
title: Activity of plant extracts on the carpogenic germination and mycelial growth of Sclerotinia sclerotiorum

description: Te efect of methanolic extracts of Annona cacans, A. coriacea, A. crassiflora, A. dioica, A. sylvatica, Geophila repens, Guettarda viburnoides, Palicourea crocea, Schinus terebinthifolius e Trichilia silvatica, and A. cacans hexane, ethyl etila, aqueous and chloroform fractions and the essential oil of S. terebinthifolius on mycelial growth and carpogenic germination of Sclerotinia sclerotiorum was evaluated. Te concentrations are 1,000 ppm for the extracts and 100 ppm for the fractions. To evaluate the germination, carpogenic, extracts and fractions were incorporated in agar-water that was poured into gerboxes where 20 sclerotia were distributed. To evaluate the mycelial growth, essential oil of S. terebinthifolius in concentrations of 0, 100 and 1,000 ppm was incorporated into the PDA and then poured into Petri dishes, to where pathogen mycelial discs were transferred. Extracts of G. repens, P. crocea and S. terebinthifolius and fractions ethyl acetate and chloroform of Annona cacans reduced the carpogenic germination of sclerotia of S. sclerotiorum and the extract of A. cacans reduced the number of apothecia formed. Mycelial growth showed 10% inhibition at the highest concentration of essential oil of S. terebinthifolius.
DOI: 10.6084/m9.figshare.6992780.v1
title: Activity of plant extracts on the carpogenic germination and mycelial growth of Sclerotinia sclerotiorum

description: Te efect of methanolic extracts of Annona cacans, A. coriacea, A. crassiflora, A. dioica, A. sylvatica, Geophila repens, Guettarda viburnoides, Palicourea crocea, Schinus terebinthifolius e Trichilia silvatica, and A. cacans hexane, ethyl etila, aqueous and chloroform fractions and the essential oil of S. terebinthifolius on mycelial growth and carpogenic germination of Sclerotinia sclerotiorum was evaluated. Te concentrations are 1,000 ppm for the extracts and 100 ppm for the fractions. To evaluate the germination, carpogenic, extracts and fractions were incorporated in agar-water that was poured into gerboxes where 20 sclerotia were distributed. To evaluate the mycelial growth, essential oil of S. terebinthifolius in concentrations of 0, 100 and 1,000 ppm was incorporated into the PDA and then poured into Petri dishes, to where pathogen mycelial discs were transferred. Extracts of G. repens, P. crocea and S. terebinthifolius and fractions ethyl acetate and chloroform of Annona cacans reduced the carpogenic germination of sclerotia of S. sclerotiorum and the extract of A. cacans reduced the number of apothecia formed. Mycelial growth showed 10% inhibition at the highest concentration of essential oil of S. terebinthifolius.
DOI: 10.6084/m9.figshare.7508771
title: Evaluation of antibiotic residues in milk samples from cows after dry cow therapy

description: The present study aimed to assess antibiotic residues in milk from animals treated in the pre-term period. A total of 47 animals from dairy farms in Rondônia, Brazil, were used for treatment with commercially available antibiotics especific to the dry period (cloxacillinbenzathine and spiramycin associated to neomycin) and during the lactation period (ceftiofur hydrochloride) at 60 and 30 days after delivery, respectively, in a single application. The detection of antimicrobial residues was performed using the commercial microbiologic kit Eclipse 50 (ZEU-Inmunotec®, Zaragoza). The results showed antibiotic residues in 3.44% of animals treated with cloxacillinbenzathine, in 12.50% of animals treated with spiramycin/neomycin, and in 10% of animals treated with ceftiofur hydrochloride. The period indicated for milk consumption after therapy was 30 days for lactation antibiotic products and 60 days for dry cow therapy. The occurrence of the antibiotic residues in milk is a risk to public health and for the contamination of milk products.
DOI: 10.6084/m9.figshare.7508771.v1
title: Evaluation of antibiotic residues in milk samples from cows after dry cow therapy

description: The present study aimed to assess antibiotic residues in milk from animals treated in the pre-term period. A total of 47 animals from dairy farms in Rondônia, Brazil, were used for treatment with commercially available antibiotics especific to the dry period (cloxacillinbenzathine and spiramycin associated to neomycin) and during the lactation period (ceftiofur hydrochloride) at 60 and 30 days after delivery, respectively, in a single application. The detection of antimicrobial residues was performed using the commercial microbiologic kit Eclipse 50 (ZEU-Inmunotec®, Zaragoza). The results showed antibiotic residues in 3.44% of animals treated with cloxacillinbenzathine, in 12.50% of animals treated with spiramycin/neomycin, and in 10% of animals treated with ceftiofur hydrochloride. The period indicated for milk consumption after therapy was 30 days for lactation antibiotic products and 60 days for dry cow therapy. The occurrence of the antibiotic residues in milk is a risk to public health and for the contamination of milk products.
DOI: 10.6084/m9.figshare.6992789
title: Evaluation of the Atibaia River water quality using Lecane bulla as a test organism

description: In view of the diversity of environments found in the Brazilian territory, it is understandable that the use of native species can provide more relevant information for ecotoxicological studies. The purpose of this work was to evaluate the quality of water samples from the Atibaia River in an area that is under the influence of petroleum refinery using a native test-organism and submitting the data to PCA statistical analysis. Therefore, acute toxicity assays with Lecane bulla (Rotifera) were performed in four locations of the river, as well as physical-chemical analyses. Sampling was drawn in the dry and rainy seasons. The bioassays were static and lasted 48 hours; dead organisms were quantified at the end of the tests. Toxicological differences among the samples/per location and control were compared by means of the Analysis of Variance. Physical-chemical and mortality variables were simultaneously analyzed by multivariate analysis of the principal components and the Pearson correlation coefficient. Water samples from the exit of the refinery stabilization pond (location S.1) were toxic to L. bulla in both seasons, with significant differences in relation to the control and between the seasons. The statistical treatment of data showed that mortality was strong and positively correlated with total hardness, chlorides and EC, which together with pH presented higher values in location S.1, in the dry and in the rainy seasons. Due to its sensibility to the quality of the Atibaia river water samples, the potential use of L. bulla for ecotoxicological studies as an alternative test organism could be demonstrated.
DOI: 10.6084/m9.figshare.6992789.v1
title: Evaluation of the Atibaia River water quality using Lecane bulla as a test organism

description: In view of the diversity of environments found in the Brazilian territory, it is understandable that the use of native species can provide more relevant information for ecotoxicological studies. The purpose of this work was to evaluate the quality of water samples from the Atibaia River in an area that is under the influence of petroleum refinery using a native test-organism and submitting the data to PCA statistical analysis. Therefore, acute toxicity assays with Lecane bulla (Rotifera) were performed in four locations of the river, as well as physical-chemical analyses. Sampling was drawn in the dry and rainy seasons. The bioassays were static and lasted 48 hours; dead organisms were quantified at the end of the tests. Toxicological differences among the samples/per location and control were compared by means of the Analysis of Variance. Physical-chemical and mortality variables were simultaneously analyzed by multivariate analysis of the principal components and the Pearson correlation coefficient. Water samples from the exit of the refinery stabilization pond (location S.1) were toxic to L. bulla in both seasons, with significant differences in relation to the control and between the seasons. The statistical treatment of data showed that mortality was strong and positively correlated with total hardness, chlorides and EC, which together with pH presented higher values in location S.1, in the dry and in the rainy seasons. Due to its sensibility to the quality of the Atibaia river water samples, the potential use of L. bulla for ecotoxicological studies as an alternative test organism could be demonstrated.
DOI: 10.6084/m9.figshare.6992801
title: Chromatographic detection of nitrofurans in foods of animal origin

description: Nitrofurans are antibacterials banned in livestock by different countries due to its relationship with the production of carcinogenic metabolites. Several studies have been conducted to find the best methodology to identify these residues. Te objectives of this review work were to show the risk of nitrofuran metabolites (furazolidone; nitrofurazone; nitrofurantoin, furaltadone and nifursol); to explain the application of liquid chromatography and mass spectrometry to determine the presence of these residues in foods of animal origin; and, finally, to report some methodologies that were recently used in different foods of animal origin.