cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12936810
title: Probabilistic model for assessing occupational risk during the handling of nanomaterials

description: Exposure to nanomaterials (NMs) can be considered as human, occupational or environmental. Occupational exposure may be experienced by the workers and/or researchers who develop and produce these products and the hazards inherent to exposure are not yet fully known. Quantitative and qualitative methods are available to estimate the occupational risks associated with the handling of NMs, however, both have limitations. In this context, the objective of this study was to create a Bayesian network (BN) that will allow an assessment of the occupational risk associated with the handling of NMs in research laboratories. The BN was developed considered variables related to exposure, the hazards associated with NMs and also the existing control measures in the work environment, such as collective protection equipment (CPE), administrative measures and personal protection equipment (PPE). In addition to assessing the occupational risk, simulations were carried out by the laboratory manager to obtain information on which actions should be taken to reduce the risk. The development of a BN to assess the occupational risk associated with the handling of NMs is a novel aspect of this study. As a distinctive feature, the BN has measurement control variables in addition to considering CPE, administrative measures and PPE. An advantage of this network in relation to other risk assessment models is that it allows the easy execution of simulations and provides a guide for a decision making by identifying which actions should be taken to minimize the risk.
DOI: 10.6084/m9.figshare.13406783
title: Inhaled multi-walled carbon nanotubes differently modulate global gene and protein expression in rat lungs

description: Inhalation of multi-walled carbon nanotubes (MWCNTs) induces lung inflammation. Depending on industrial applications, CNTs with different physicochemical characteristics are produced and workers can potentially be exposed. This raises concerns about the long-term health effects of these nanomaterials. Because of the wide variety of MWCNTs, it is essential to study the toxicological effects of CNTs of various shapes and to better understand the impact physical and chemical properties have on their toxicity. In this study, rats were exposed by nose-only to two pristine MWCNTs with different morphologies: the long and thick NM-401 or the short and thin NM-403. After four weeks of inhalation, animals were euthanized at four different times during the recovery period: three days (short-term), 30 and 90 days (intermediate-term) and 180 days (long-term). Analyses of the transcriptome in the whole lung and the proteome in the bronchoalveolar lavage fluid of exposed animals were performed to understand the MWCNT underlying mechanisms of toxicity. Following inhalation of NM-401, we observed a dose-dependent increase in the number of differentially expressed genes and proteins, whereas there is no clear difference between the two concentrations of NM-403. After NM-403 inhalation, the number of differentially expressed genes and proteins varied less between the four post-exposure times compared to NM-401, which supports the postulation of a persistent effect of this type of CNT. Our toxicogenomics approaches give insights into the different toxicological profile following MWCNT exposure.
DOI: 10.6084/m9.figshare.13406783.v1
title: Inhaled multi-walled carbon nanotubes differently modulate global gene and protein expression in rat lungs

description: Inhalation of multi-walled carbon nanotubes (MWCNTs) induces lung inflammation. Depending on industrial applications, CNTs with different physicochemical characteristics are produced and workers can potentially be exposed. This raises concerns about the long-term health effects of these nanomaterials. Because of the wide variety of MWCNTs, it is essential to study the toxicological effects of CNTs of various shapes and to better understand the impact physical and chemical properties have on their toxicity. In this study, rats were exposed by nose-only to two pristine MWCNTs with different morphologies: the long and thick NM-401 or the short and thin NM-403. After four weeks of inhalation, animals were euthanized at four different times during the recovery period: three days (short-term), 30 and 90 days (intermediate-term) and 180 days (long-term). Analyses of the transcriptome in the whole lung and the proteome in the bronchoalveolar lavage fluid of exposed animals were performed to understand the MWCNT underlying mechanisms of toxicity. Following inhalation of NM-401, we observed a dose-dependent increase in the number of differentially expressed genes and proteins, whereas there is no clear difference between the two concentrations of NM-403. After NM-403 inhalation, the number of differentially expressed genes and proteins varied less between the four post-exposure times compared to NM-401, which supports the postulation of a persistent effect of this type of CNT. Our toxicogenomics approaches give insights into the different toxicological profile following MWCNT exposure.
DOI: 10.6084/m9.figshare.13383005
title: ‘Trojan-Horse’ stress-granule formation mediated by manganese oxide nanoparticles

description: Exposure to nanomaterials is considered as one of the risk factors for neurodegenerative pathology. <i>In vitro</i> inorganic nanoparticles (NPs) absorb intrinsically disordered proteins, many of which are the constituents of stress-granules (SGs). SGs normally form in response to cellular stress and, here, we addressed whether selected inorganic NPs could trigger SGs formation in cells. To this end, we have tested a series of inorganic NPs for their ability to induce SGs formation in human glioblastoma and fibroblast cell lines. Among tested NPs, only Mn<sub>3</sub>O<sub>4</sub> NPs triggered SGs formation in cell-type-specific and metabolic-dependent manner. In human glioblastoma U87 MG cell line, Mn<sub>3</sub>O<sub>4</sub> NPs entered cells within minutes and resided inside intracellular vesicles for at least 48 h. Mn<sub>3</sub>O<sub>4</sub> NPs induced a strong reduction in oxidative phosphorylation rate, but not glycolysis. We showed that Mn<sub>3</sub>O<sub>4</sub> NPs slowly dissolve producing a local net of Mn<sup>2+</sup> cations, which are known to inhibit oxidative phosphorylation. Indeed, direct incubation of cells with equimolar amounts of Mn<sup>2+</sup> cations triggered SGs formation and reduced cellular respiration rate. However, while SGs formed in response to Mn<sub>3</sub>O<sub>4</sub> NPs persisted for hours, SGs formation by Mn<sup>2+</sup> peaked and dropped within minutes. Finally, Mn<sub>3</sub>O<sub>4</sub> NPs mediated SGs formation via the phosphorylation of eIF2α. Thus, we conclude that exposure of U87 MG cells to Mn<sub>3</sub>O<sub>4</sub> NPs caused a ‘Trojan-horse’ prolonged SGs response.
DOI: 10.6084/m9.figshare.13383005.v1
title: ‘Trojan-Horse’ stress-granule formation mediated by manganese oxide nanoparticles

description: Exposure to nanomaterials is considered as one of the risk factors for neurodegenerative pathology. <i>In vitro</i> inorganic nanoparticles (NPs) absorb intrinsically disordered proteins, many of which are the constituents of stress-granules (SGs). SGs normally form in response to cellular stress and, here, we addressed whether selected inorganic NPs could trigger SGs formation in cells. To this end, we have tested a series of inorganic NPs for their ability to induce SGs formation in human glioblastoma and fibroblast cell lines. Among tested NPs, only Mn<sub>3</sub>O<sub>4</sub> NPs triggered SGs formation in cell-type-specific and metabolic-dependent manner. In human glioblastoma U87 MG cell line, Mn<sub>3</sub>O<sub>4</sub> NPs entered cells within minutes and resided inside intracellular vesicles for at least 48 h. Mn<sub>3</sub>O<sub>4</sub> NPs induced a strong reduction in oxidative phosphorylation rate, but not glycolysis. We showed that Mn<sub>3</sub>O<sub>4</sub> NPs slowly dissolve producing a local net of Mn<sup>2+</sup> cations, which are known to inhibit oxidative phosphorylation. Indeed, direct incubation of cells with equimolar amounts of Mn<sup>2+</sup> cations triggered SGs formation and reduced cellular respiration rate. However, while SGs formed in response to Mn<sub>3</sub>O<sub>4</sub> NPs persisted for hours, SGs formation by Mn<sup>2+</sup> peaked and dropped within minutes. Finally, Mn<sub>3</sub>O<sub>4</sub> NPs mediated SGs formation via the phosphorylation of eIF2α. Thus, we conclude that exposure of U87 MG cells to Mn<sub>3</sub>O<sub>4</sub> NPs caused a ‘Trojan-horse’ prolonged SGs response.
DOI: 10.6084/m9.figshare.13089435
title: Responses to iron oxide and zinc oxide nanoparticles in echinoderm embryos and microalgae: uptake, growth, morphology, and transcriptomic analysis

description: We investigated the toxicity of Iron oxide and Zinc oxide engineered nanoparticles (ENPs) on <i>Paracentrotus lividus</i> sea urchin embryos and three species of microalgae. Morphological responses, internalization, and potential impacts of Fe<sub>2</sub>O<sub>3</sub> and ZnO ENPs on physiology and metabolism were assessed. Both types of ENPs affected <i>P. lividus</i> larval development, but ZnO ENPs had a much stronger effect. While growth of the alga <i>Micromonas commoda</i> was severely impaired by both ENPs, <i>Ostreococcus tauri</i> or <i>Nannochloris sp.</i> were unaffected. Transmission electron microscopy showed the internalization of ENPs in sea urchin embryonic cells while only nanoparticle interaction with external membranes was evidenced in microalgae, suggesting that marine organisms react in diverse ways to ENPs. Transcriptome-wide analysis in <i>P. lividus</i> and <i>M. commoda</i> showed that many different physiological pathways were affected, some of which were common to both species, giving insights about the mechanisms underpinning toxic responses.
DOI: 10.6084/m9.figshare.13089435.v3
title: Responses to iron oxide and zinc oxide nanoparticles in echinoderm embryos and microalgae: uptake, growth, morphology, and transcriptomic analysis

description: We investigated the toxicity of Iron oxide and Zinc oxide engineered nanoparticles (ENPs) on <i>Paracentrotus lividus</i> sea urchin embryos and three species of microalgae. Morphological responses, internalization, and potential impacts of Fe<sub>2</sub>O<sub>3</sub> and ZnO ENPs on physiology and metabolism were assessed. Both types of ENPs affected <i>P. lividus</i> larval development, but ZnO ENPs had a much stronger effect. While growth of the alga <i>Micromonas commoda</i> was severely impaired by both ENPs, <i>Ostreococcus tauri</i> or <i>Nannochloris sp.</i> were unaffected. Transmission electron microscopy showed the internalization of ENPs in sea urchin embryonic cells while only nanoparticle interaction with external membranes was evidenced in microalgae, suggesting that marine organisms react in diverse ways to ENPs. Transcriptome-wide analysis in <i>P. lividus</i> and <i>M. commoda</i> showed that many different physiological pathways were affected, some of which were common to both species, giving insights about the mechanisms underpinning toxic responses.
DOI: 10.6084/m9.figshare.13089435.v2
title: Responses to iron oxide and zinc oxide nanoparticles in echinoderm embryos and microalgae: uptake, growth, morphology, and transcriptomic analysis

description: We investigated the toxicity of Iron oxide and Zinc oxide engineered nanoparticles (ENPs) on <i>Paracentrotus lividus</i> sea urchin embryos and three species of microalgae. Morphological responses, internalization, and potential impacts of Fe<sub>2</sub>O<sub>3</sub> and ZnO ENPs on physiology and metabolism were assessed. Both types of ENPs affected <i>P. lividus</i> larval development, but ZnO ENPs had a much stronger effect. While growth of the alga <i>Micromonas commoda</i> was severely impaired by both ENPs, <i>Ostreococcus tauri</i> or <i>Nannochloris sp.</i> were unaffected. Transmission electron microscopy showed the internalization of ENPs in sea urchin embryonic cells while only nanoparticle interaction with external membranes was evidenced in microalgae, suggesting that marine organisms react in diverse ways to ENPs. Transcriptome-wide analysis in <i>P. lividus</i> and <i>M. commoda</i> showed that many different physiological pathways were affected, some of which were common to both species, giving insights about the mechanisms underpinning toxic responses.
DOI: 10.25345/c5qf9x
title: MassIVE MSV000087390 - The ion channel TRPM7 regulates zinc depletion-induced MDMX degradation
title: Identification of the proteins interacted with both MDM2 and MDMX
DOI: 10.15161/oar.it/73772
title: CovidStat project data

description: This record contains the daily updated data of the CovidStat project. CovidStat is a project carried out by the CovidStat Working Group at INFN, whose creation was promoted within the Italian National Institute of Nuclear Physics with the aim of making a statistical analysis of the data provided daily by the Civil Protection on the spread of the pandemic in Italy. CovidStat data can interactively be browsed at https://covid19.infn.it.
description: The software to process this Root TTree is in the related identifier. Be sure to use the right corresponding version, based on the data in which Root TTree was generated.
DOI: 10.5281/zenodo.4743545
title: Data for "Coherent spin qubit transport in silicon"

description: This dataset contains the data files for "Coherent spin qubit transport in silicon".
DOI: 10.5281/zenodo.4743544
title: Data for "Coherent spin qubit transport in silicon"

description: This dataset contains the data files for "Coherent spin qubit transport in silicon".
DOI: 10.6084/m9.figshare.11925849
title: Global and regional connectivity analysis of resting-state function MRI brain images using graph theory in Parkinson’s disease

description: Parkinson’s disease (PD) is the second most common neurodegenerative disorder which resists around 10 million people worldwide. It develops when nerve cells in a region of the brain that regulates movement become damaged; the symptoms usually begin gradually and become critical over time. In this study, we proposed to investigate the topological properties of functional brain networks within healthy controls (HCs) and PD patients. Also, we evaluated the gender difference among PD patients through graph theoretical approach. The rs-fMRI (resting-state functional magnetic resonance imaging) data of fifty-one PD patients and healthy controls was applied to generate the brain functional connectome. The functional whole-brain connectome was constructed by thresholding partial correlation matrices of 160 regions from Dosenbach brain atlas. From the graph theory approach, global and nodal metrics were analysed, and we observed considerable changes in PD patients in comparison with healthy controls. Findings suggest that there is a significant difference in the topological characteristics of PD patients, and this was found to be evident in the default mode network (DMN) and occipital regions. This study provides essential insights from network changes to the clinically relevant information for the PD progression.
DOI: 10.6084/m9.figshare.11925849.v1
title: Global and regional connectivity analysis of resting-state function MRI brain images using graph theory in Parkinson’s disease

description: Parkinson’s disease (PD) is the second most common neurodegenerative disorder which resists around 10 million people worldwide. It develops when nerve cells in a region of the brain that regulates movement become damaged; the symptoms usually begin gradually and become critical over time. In this study, we proposed to investigate the topological properties of functional brain networks within healthy controls (HCs) and PD patients. Also, we evaluated the gender difference among PD patients through graph theoretical approach. The rs-fMRI (resting-state functional magnetic resonance imaging) data of fifty-one PD patients and healthy controls was applied to generate the brain functional connectome. The functional whole-brain connectome was constructed by thresholding partial correlation matrices of 160 regions from Dosenbach brain atlas. From the graph theory approach, global and nodal metrics were analysed, and we observed considerable changes in PD patients in comparison with healthy controls. Findings suggest that there is a significant difference in the topological characteristics of PD patients, and this was found to be evident in the default mode network (DMN) and occipital regions. This study provides essential insights from network changes to the clinically relevant information for the PD progression.
DOI: 10.6084/m9.figshare.13141319
title: Single nucleotide polymorphisms of taste genes and caries: a systematic review and meta-analysis

description: The aim of the present study was to systematically review the literature investigating the single nucleotide polymorphisms (SNP) related to taste genes and their influence on caries. Search was performed in five databases to respond to the question: ‘Are the polymorphisms of taste genes associated with dental caries?’. Studies in humans were included. Assessment of quality of studies, meta-analysis and sensitivity analysis were performed. Seven studies were included in the systematic review and two in meta-analysis. Most of studies (71.4%) presented cohort design with low-level of evidence. A total of 4,032 individuals were evaluated. Four different taste genes (<i>TAS1R2, TAS2R38, TAS1R3</i> and <i>GLUT2</i>) and 12 SNPs were reported. Most SNPs of taste genes showed a protective effect of the minor allele against dental caries. Meta-analysis included the SNP rs713598 placed in the <i>TAS2R38</i> gene. The results suggest an effect of the heterozygote genotype (CG), which was associate with low caries experience (OR = 0.35 CI95% [0.17–0.75]). However, the genotype GG was not associated (OR = 0.17 CI95% [0.03–1.04]). Sensitivity analysis showed an important influence of one study in the results. SNP of taste genes seems to be associated with caries experience. Causal inferences should be interpreted with caution and the results must be replicated in different populations.
DOI: 10.6084/m9.figshare.12915740
title: The reliability and validity of the Romanian rapid estimate of adult literacy in dentistry (RREALD-30)

description: This study aimed to translate and adapt the Rapid Estimate of Adult Literacy in Dentistry (RREALD-30) instrument for Romanian urban adults and to test its reliability and validity for oral health literacy studies. The study examined urban adult patients (<i>n</i> = 224) who attended the dental school clinic at the Faculty of Dental Medicine, Bucharest. We collected data through face-to-face interviews utilising the REALD-30 instrument. The interviews enquired about the Oral Health Impact Profile (OHIP-14), background characteristics, oral health-related knowledge, visits to dentists and self-rated oral health status. We applied principal component analysis for factor structure and Item Response Theory models to discriminate ability. A structural equation model (SEM) evaluated whether knowledge, perceived oral health, and visits to the dentist mediate the effect of RREALD on OHIP-14. Of the 224 participants, 113 (50.4%) were males. The internal consistency of the RREALD-30 measured by Cronbach’s alpha was 0.88. The test-retest reliability was excellent (Spearman’s correlation coefficient 0.98, ICC 0.90). RREALD-30 exhibited good concurrent and predictive validity. SEM demonstrated that RREALD mediated the effect of visits to dentist on OHIP-14. The RREALD-30 proved satisfactory psychometric properties and may serve to evaluate dental health literacy among Romanian adults.
DOI: 10.6084/m9.figshare.12915740.v1
title: The reliability and validity of the Romanian rapid estimate of adult literacy in dentistry (RREALD-30)

description: This study aimed to translate and adapt the Rapid Estimate of Adult Literacy in Dentistry (RREALD-30) instrument for Romanian urban adults and to test its reliability and validity for oral health literacy studies. The study examined urban adult patients (<i>n</i> = 224) who attended the dental school clinic at the Faculty of Dental Medicine, Bucharest. We collected data through face-to-face interviews utilising the REALD-30 instrument. The interviews enquired about the Oral Health Impact Profile (OHIP-14), background characteristics, oral health-related knowledge, visits to dentists and self-rated oral health status. We applied principal component analysis for factor structure and Item Response Theory models to discriminate ability. A structural equation model (SEM) evaluated whether knowledge, perceived oral health, and visits to the dentist mediate the effect of RREALD on OHIP-14. Of the 224 participants, 113 (50.4%) were males. The internal consistency of the RREALD-30 measured by Cronbach’s alpha was 0.88. The test-retest reliability was excellent (Spearman’s correlation coefficient 0.98, ICC 0.90). RREALD-30 exhibited good concurrent and predictive validity. SEM demonstrated that RREALD mediated the effect of visits to dentist on OHIP-14. The RREALD-30 proved satisfactory psychometric properties and may serve to evaluate dental health literacy among Romanian adults.
DOI: 10.6084/m9.figshare.13141319.v1
title: Single nucleotide polymorphisms of taste genes and caries: a systematic review and meta-analysis

description: The aim of the present study was to systematically review the literature investigating the single nucleotide polymorphisms (SNP) related to taste genes and their influence on caries. Search was performed in five databases to respond to the question: ‘Are the polymorphisms of taste genes associated with dental caries?’. Studies in humans were included. Assessment of quality of studies, meta-analysis and sensitivity analysis were performed. Seven studies were included in the systematic review and two in meta-analysis. Most of studies (71.4%) presented cohort design with low-level of evidence. A total of 4,032 individuals were evaluated. Four different taste genes (<i>TAS1R2, TAS2R38, TAS1R3</i> and <i>GLUT2</i>) and 12 SNPs were reported. Most SNPs of taste genes showed a protective effect of the minor allele against dental caries. Meta-analysis included the SNP rs713598 placed in the <i>TAS2R38</i> gene. The results suggest an effect of the heterozygote genotype (CG), which was associate with low caries experience (OR = 0.35 CI95% [0.17–0.75]). However, the genotype GG was not associated (OR = 0.17 CI95% [0.03–1.04]). Sensitivity analysis showed an important influence of one study in the results. SNP of taste genes seems to be associated with caries experience. Causal inferences should be interpreted with caution and the results must be replicated in different populations.
DOI: 10.6084/m9.figshare.14559987
title: Sand, water and air temperature data from Impact of marine heatwaves for sea turtle nest temperatures

description: Mean monthly sand, air and water temperatures.
DOI: 10.6084/m9.figshare.14559987.v1
title: Sand, water and air temperature data from Impact of marine heatwaves for sea turtle nest temperatures

description: Mean monthly sand, air and water temperatures.