cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6943841.v1
title: Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

description: ABSTRACT Plant growth-promoting bacteria (PGPB) comprise part of plant microbiome of biotechnological interest due to their potential to decrease the use of agrochemicals in agriculture. Among the commonly found PGPB species, the Pseudomonas genus is known for high competitiveness and efficiency in expressing growth-promotion traits. To increase the contribution of diazotrophic Pseudomonas sp. to the plant nitrogen nutrition, the strain AZM-01 was chemically mutagenized with methyl methanesulfonate (MMS), following the selection for resistance to ethylenediamine (EDA). From the 13 EDA-resistant mutant strains selected, four showed increased the ammonium excretion, with the highest value reaching up to 284% increase as compared to the wild strain, and six strains were found to produce significantly more auxins than the wild strain. Two independent inoculation trials with the wild and EDA-resistant Pseudomonas were performed on maize, with the objective to study the influence of bacteria on seed germination and its potential to promote maize growth under N-limiting condition. In general, Pseudomonas inoculation modified the root architecture of germinating seeds, and increased biomass of maize plants grown under N-limiting conditions. Shoot dry weight of maize was increased by inoculation with several EDA-resistant mutants as compared to the strain AZM-01, with emphasis on the EDA-5 strain which supports biomass accumulation at equivalent amount of plants grown under full N supply. Significant correlations between in vitro and in vivo parameters were found although low coefficient values predominate. The strategy of random mutagenesis was found suitable to develop PGPB strains with higher potential to supply maize plants with nitrogen.
DOI: 10.6084/m9.figshare.6943844.v1
title: Methodologies for selecting cassava with resistance to dry and black root rot under controlled conditions

description: ABSTRACT Cassava root rot diseases such as dry and black root rot are listed among the major threats, since its affect the main product (tuberous roots), causing a progressive decline in yield and affecting subsequent crop cycles, being the use of resistant varieties the most reliable control measure on field level. The objective of this study was to identify inoculation methods for the early evaluation of genotypes, considering the level of resistance to dry (DRR) and black (BRR) root rot diseases. Different methodologies and plant tissues were evaluated, based on the immersion of cassava tissues (roots and stem cuttings), soil infestation, and inoculation of detached tissues (leaves, tuberous roots and stem cuttings). The following parameters were evaluated for inoculations based on tissue immersion: disease index (ω); aerial part weight (g); fresh weight of the roots (g); and volume (cm3). For the inoculations on detached tissues, the percentage of lesioned area was determined. Immersion methods for roots and lesioned stems did not show typical symptoms of DRR and BRR during the two-month evaluation period. The soil infestation method did not differ from the stem immersion method as to the reduction of aerial part weight and the disease index, whereas both can be recommended for resistance selection trials. There was a positive correlation between the BRR and DRR data for the stem inoculation (r = 0.94, p = 0.001) and for DRR in the peel and root pulp (r = 0.73, p = 0.05). Therefore, the resistance within each tissue is apparently independent and should be compared with the behavior of the genotypes in the field.
DOI: 10.6084/m9.figshare.6943829
title: Educational technologies and practices for prevention of vertical HIV transmission

description: ABSTRACT Objective: to assess available evidence on educational technologies and practices for prevention of vertical HIV transmission. Method: LILACS, PubMed, Scopus, BDENF, between April and May 2016, with the descriptors: “Vertical Transmission of Infectious Disease”, “HIV”, “Health Education” and “Technology”. Results: there are 16 articles published between 2000 and 2014, mostly Brazilian and African, Cross-sectional and with low level of evidence. The studies covered the use of hard technologies, through video, radio and telephone, and soft, emphasizing, in particular, counseling. Conclusion: the studies recognize the importance of educational activities as a tool for health promotion in the context of vertical HIV transmission, despite reporting the need for constant training of professionals and urgency in the renewal of educational concepts and practices. Therefore, it is recommended to expand and consolidate health counseling and emphasize the role of nurses as an important actor in this setting.
DOI: 10.6084/m9.figshare.6943829.v1
title: Educational technologies and practices for prevention of vertical HIV transmission

description: ABSTRACT Objective: to assess available evidence on educational technologies and practices for prevention of vertical HIV transmission. Method: LILACS, PubMed, Scopus, BDENF, between April and May 2016, with the descriptors: “Vertical Transmission of Infectious Disease”, “HIV”, “Health Education” and “Technology”. Results: there are 16 articles published between 2000 and 2014, mostly Brazilian and African, Cross-sectional and with low level of evidence. The studies covered the use of hard technologies, through video, radio and telephone, and soft, emphasizing, in particular, counseling. Conclusion: the studies recognize the importance of educational activities as a tool for health promotion in the context of vertical HIV transmission, despite reporting the need for constant training of professionals and urgency in the renewal of educational concepts and practices. Therefore, it is recommended to expand and consolidate health counseling and emphasize the role of nurses as an important actor in this setting.
DOI: 10.6084/m9.figshare.6943823
title: Modified segmental vertebral stabilization in the treatment of spinal cord trauma in a Paraguaian hairy dwarf porcupine (Coendou spinosus): case report

description: ABSTRACT An female, adult Paraguayan hairy dwarf porcupine (Coendou spinosus, F. Cuvier, 1823) was referred to the Veterinary Hospital with clinical history of spinal cord injury. In the neurological examination, paraplegia with preserved nociception compatible with signs of spinal shock were observed. Radiographic and myelographic examinations showed evidence of a vertebral dislocation and fracture between the 10th and 11th thoracic vertebrae. The patient underwent surgical stabilization of the lesion using the modified segmental vertebral stabilization technique with Steinmann pins and cerclage wires. Under the conditions reported in this case, the technique was effective in stabilizing vertebral fracture/dislocation and in recovering neurological functions. The patient presented progressive recovery from ambulation. To the authors’ knowledge, this is the first case report that performed modified segment stabilization for thoracic vertebral fracture/luxation treatment in Coendou spinosus.
DOI: 10.6084/m9.figshare.6943823.v1
title: Modified segmental vertebral stabilization in the treatment of spinal cord trauma in a Paraguaian hairy dwarf porcupine (Coendou spinosus): case report

description: ABSTRACT An female, adult Paraguayan hairy dwarf porcupine (Coendou spinosus, F. Cuvier, 1823) was referred to the Veterinary Hospital with clinical history of spinal cord injury. In the neurological examination, paraplegia with preserved nociception compatible with signs of spinal shock were observed. Radiographic and myelographic examinations showed evidence of a vertebral dislocation and fracture between the 10th and 11th thoracic vertebrae. The patient underwent surgical stabilization of the lesion using the modified segmental vertebral stabilization technique with Steinmann pins and cerclage wires. Under the conditions reported in this case, the technique was effective in stabilizing vertebral fracture/dislocation and in recovering neurological functions. The patient presented progressive recovery from ambulation. To the authors’ knowledge, this is the first case report that performed modified segment stabilization for thoracic vertebral fracture/luxation treatment in Coendou spinosus.
DOI: 10.6084/m9.figshare.6943817
title: Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities

description: Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, respectively. Moreover, small interfering RNA (siRNA) plasmid was used to examine the role of p53 in icariin-induced apoptosis in HCT116 cells. Icariin significantly suppressed colon carcinoma HCT116 cells by decreasing migration and viability, and simultaneously promoting apoptosis. Icariin exerted the anti-tumor effect in a dose-dependent manner by up-regulating p53. During treatment of icariin, p-p53, p21, and Bax levels increased, and Bcl-2 level decreased. Short time treatment with icariin induced DNA damage in HCT116 cells. Furthermore, the cytotoxicity of icariin was decreased after p53 knockdown or by using caspase inhibitors. p53 was involved in activities of caspase-9 and caspase-3. Icariin repressed colon carcinoma cell line HCT116 by enhancing p53 expression and activating p53 functions possibly through Bcl-2/Bax imbalance and caspase-9 and -3 regulation. Icariin treatment also induced DNA damage in HCT116 cells.
DOI: 10.6084/m9.figshare.6943820
title: HISTORICAL AND POLITICAL EDUCATION COURSE FOR UNDERGRADUATE HEALTH STUDENTS: ANALYSIS OF A SHARED CONSTRUCTION

description: Abstract Undergraduate studies in the fields of health are currently being debated regarding the need for change in their syllabuses concerning the principles of the Brazilian Unified Health System, among other topics. Overcoming the operational and technicist education and expanding the critical thinking abilities of the students about the historical and political determinations of health are among the challenges highlighted. The article analyzes the teaching/learning process with a critical perspective based on the Historical and Political Education Course for Undergraduate Health Students, an extension semi-virtual course offered nationally, and supported by the National Policy for Popular Education in Health within the Unified Health System. In the present article, we analyzed the experiences brought about by the course, which took place between March and December 2014. The development of the course was based on the theoretical and methodological conceptions of the construction of shared knowledge, guided by the field of Popular Education and Health. The article presents: the history and the process of development of the course, the political and pedagogical project, the educational and pedagogical materials, and the contributions and questions, with notes on the potential future implications in educational processes in the field of health.
DOI: 10.6084/m9.figshare.6943817.v1
title: Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities

description: Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, respectively. Moreover, small interfering RNA (siRNA) plasmid was used to examine the role of p53 in icariin-induced apoptosis in HCT116 cells. Icariin significantly suppressed colon carcinoma HCT116 cells by decreasing migration and viability, and simultaneously promoting apoptosis. Icariin exerted the anti-tumor effect in a dose-dependent manner by up-regulating p53. During treatment of icariin, p-p53, p21, and Bax levels increased, and Bcl-2 level decreased. Short time treatment with icariin induced DNA damage in HCT116 cells. Furthermore, the cytotoxicity of icariin was decreased after p53 knockdown or by using caspase inhibitors. p53 was involved in activities of caspase-9 and caspase-3. Icariin repressed colon carcinoma cell line HCT116 by enhancing p53 expression and activating p53 functions possibly through Bcl-2/Bax imbalance and caspase-9 and -3 regulation. Icariin treatment also induced DNA damage in HCT116 cells.
DOI: 10.6084/m9.figshare.6943820.v1
title: HISTORICAL AND POLITICAL EDUCATION COURSE FOR UNDERGRADUATE HEALTH STUDENTS: ANALYSIS OF A SHARED CONSTRUCTION

description: Abstract Undergraduate studies in the fields of health are currently being debated regarding the need for change in their syllabuses concerning the principles of the Brazilian Unified Health System, among other topics. Overcoming the operational and technicist education and expanding the critical thinking abilities of the students about the historical and political determinations of health are among the challenges highlighted. The article analyzes the teaching/learning process with a critical perspective based on the Historical and Political Education Course for Undergraduate Health Students, an extension semi-virtual course offered nationally, and supported by the National Policy for Popular Education in Health within the Unified Health System. In the present article, we analyzed the experiences brought about by the course, which took place between March and December 2014. The development of the course was based on the theoretical and methodological conceptions of the construction of shared knowledge, guided by the field of Popular Education and Health. The article presents: the history and the process of development of the course, the political and pedagogical project, the educational and pedagogical materials, and the contributions and questions, with notes on the potential future implications in educational processes in the field of health.
DOI: 10.6084/m9.figshare.6943811
title: Clinical simulation to teach nursing care for wounded patients

description: ABSTRACT Objective: to report the experience of constructing and applicating clinical simulation scenarios for the evaluation and treatment of wounds. Method: experience report on two simulation scenarios for nursing care of wounded patients applied to nursing undergraduates. We structured simulations based on the model from the National League for Nursing/Jeffries Simulation Framework. The scenarios were evaluated by the instrument Simulation Design Scale and the students by the experience with the simulation. Results: the scenarios reproduced nursing care situations with the application of role play and moulage, which allowed us to evaluate and discuss the wound treatment. Reflections on the debriefing were important for the teaching-learning process and association between theory and practice, these factors determined the satisfaction of students with the activity. Conclusion: using clinical simulation scenarios to teach students favored the clinical reasoning and decision-making in the evaluation and treatment of wounds.
DOI: 10.6084/m9.figshare.6943808
title: A CBCT evaluation of molar uprighting by conventional versus microimplant-assisted methods: an in-vivo study

description: ABSTRACT Objective: The aim of this prospective study was to compare the three-dimensional effects of the conventional helical uprighting spring (CA) and the mini-implant assisted helical uprighting spring (MIA), using CBCT scans. Methods: Twenty patients with mesially tipped second mandibular molars were divided into two groups: CA group, in which 10 patients were treated using a conventional helical uprighting spring with conventional anchorage; and MIA group, in which 10 patients were treated using a mini-implant supported uprighting spring. Molar uprighting was observed in both groups for a period of four months. Two standardized 11×5-cm CBCT sections of the mandible were taken, being one prior to uprighting and one at the end of the four month follow-up. Statistical analyses at the beginning of treatment and after a 4 month follow-up were performed, with a significance level of p&lt; 0.05. Results: The mean amount of change in mesiodistal angulation in the MIA group was 8.53 ± 2.13o (p&lt; 0.001) and in the CA group was 9.8 ± 0.5o (p&lt; 0 .001). Statistically significant differences were found between the two groups with regard to buccolingual inclination of canine, first and second premolars (p&lt; 0.05), second molar (p&lt; 0.001) and extrusion of second molar (p&lt; 0.05). Conclusions: The mean amount of change in the mesial angulation of the second molar in the CA as well as the MIA groups was similar. MIA, which used mini-implant as a source of anchorage, was more effective in preventing movement of the anchorage teeth as well as preventing extrusion of the second molar in the vertical plane, when compared to the CA group, which used dental units as a source of anchorage.
DOI: 10.6084/m9.figshare.6943808.v1
title: A CBCT evaluation of molar uprighting by conventional versus microimplant-assisted methods: an in-vivo study

description: ABSTRACT Objective: The aim of this prospective study was to compare the three-dimensional effects of the conventional helical uprighting spring (CA) and the mini-implant assisted helical uprighting spring (MIA), using CBCT scans. Methods: Twenty patients with mesially tipped second mandibular molars were divided into two groups: CA group, in which 10 patients were treated using a conventional helical uprighting spring with conventional anchorage; and MIA group, in which 10 patients were treated using a mini-implant supported uprighting spring. Molar uprighting was observed in both groups for a period of four months. Two standardized 11×5-cm CBCT sections of the mandible were taken, being one prior to uprighting and one at the end of the four month follow-up. Statistical analyses at the beginning of treatment and after a 4 month follow-up were performed, with a significance level of p&lt; 0.05. Results: The mean amount of change in mesiodistal angulation in the MIA group was 8.53 ± 2.13o (p&lt; 0.001) and in the CA group was 9.8 ± 0.5o (p&lt; 0 .001). Statistically significant differences were found between the two groups with regard to buccolingual inclination of canine, first and second premolars (p&lt; 0.05), second molar (p&lt; 0.001) and extrusion of second molar (p&lt; 0.05). Conclusions: The mean amount of change in the mesial angulation of the second molar in the CA as well as the MIA groups was similar. MIA, which used mini-implant as a source of anchorage, was more effective in preventing movement of the anchorage teeth as well as preventing extrusion of the second molar in the vertical plane, when compared to the CA group, which used dental units as a source of anchorage.
DOI: 10.6084/m9.figshare.6943805
title: Liberating critical pedagogy of Paulo Freire in the scientific production of Nursing 1990-2017

description: ABSTRACT Objective: To analyze the theoretical and methodological application of Paulo Freire’s critical pedagogy in the scientific production of nursing. Method: An integrative review was carried out with consultation of the databases: LILACS, BDENF, MEDLINE, PUDMED and CINHAL. We included studies in the Spanish, English and Portuguese languages, published from 1990 to 2017. Results: A total of 38 articles were analyzed, of which the main concepts adopted were: dialogue/dialogicity, awareness/critical awareness and questioning. Regarding the application of the method, it was noticed the predominance of the adoption of elements such as culture circles, thematic phase and horizontality relation of the nurse with the individuals involved. Conclusion: Nursing has partially appropriated the Freire’s referential. However, it reveals the intentionality of a transformative practice that requires deepening for the implementation of the method in its full.
DOI: 10.6084/m9.figshare.6943811.v1
title: Clinical simulation to teach nursing care for wounded patients

description: ABSTRACT Objective: to report the experience of constructing and applicating clinical simulation scenarios for the evaluation and treatment of wounds. Method: experience report on two simulation scenarios for nursing care of wounded patients applied to nursing undergraduates. We structured simulations based on the model from the National League for Nursing/Jeffries Simulation Framework. The scenarios were evaluated by the instrument Simulation Design Scale and the students by the experience with the simulation. Results: the scenarios reproduced nursing care situations with the application of role play and moulage, which allowed us to evaluate and discuss the wound treatment. Reflections on the debriefing were important for the teaching-learning process and association between theory and practice, these factors determined the satisfaction of students with the activity. Conclusion: using clinical simulation scenarios to teach students favored the clinical reasoning and decision-making in the evaluation and treatment of wounds.
DOI: 10.6084/m9.figshare.6943805.v1
title: Liberating critical pedagogy of Paulo Freire in the scientific production of Nursing 1990-2017

description: ABSTRACT Objective: To analyze the theoretical and methodological application of Paulo Freire’s critical pedagogy in the scientific production of nursing. Method: An integrative review was carried out with consultation of the databases: LILACS, BDENF, MEDLINE, PUDMED and CINHAL. We included studies in the Spanish, English and Portuguese languages, published from 1990 to 2017. Results: A total of 38 articles were analyzed, of which the main concepts adopted were: dialogue/dialogicity, awareness/critical awareness and questioning. Regarding the application of the method, it was noticed the predominance of the adoption of elements such as culture circles, thematic phase and horizontality relation of the nurse with the individuals involved. Conclusion: Nursing has partially appropriated the Freire’s referential. However, it reveals the intentionality of a transformative practice that requires deepening for the implementation of the method in its full.
DOI: 10.6084/m9.figshare.6943799
title: Training in diabetes education: meanings attributed by primary care nurses

description: ABSTRACT Objective: seize meanings attributed by primary care nurses to training in diabetes education. Method: exploratory and descriptive study, with a qualitative approach, with twenty primary care nurses; semistructured interview script, with interviews processed in the IRaMuTeQ software and analyzed through the Descending Hierarchical Classification. The results were subsidized in the Representational Theory of Meaning. Results: nurse training in diabetes education is insufficient for holistic action, although it allows the community to be instrumentalized in specific issues about the disease, using the limited tools available, especially lectures. Nurses find themselves in a context of challenges, improvisations, weaknesses, and limitations that determine the meaning attributed to diabetes education and subsequent actions. Conclusion: the meanings attributed by the nurses revealed an incipient training, which limits the quality of care provided and instigates the search for qualification.