DOI: 10.22002/mkc4e-d2827
title: Rhodopseudomonas palustrus

description: Fe-metabolizing bacterium that makes parallel membrane sheets for photosynthesis.  Membrane topology is the primary interest.
description: <strong>Tilt Series Date:</strong> 2007-10-04</p>
<strong>Data Taken By:</strong> Bill Tivol</p>
<strong>Species / Specimen:</strong> Rhodopseudomonas palustris</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1 deg°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Diane Newman, PI, Lars Dietrich, Postdoc, Lars gave me the specimens</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in FEM medium +10% acetate at 30 deg C in bright light.</p>
<strong>Sample Preparation:</strong> quantifoil, 10 nm gold, 100% humidity</p>
  cons1tomo8.jpg: connections between membrane sacks
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-10-04-3/rawdata/</p>Rp4Oct07_08.mrc,  Tilt Series (Pixel Size 0.625 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-10-04-3/rawdata/Rp4Oct07_08.mrc"
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        Rp4Oct07_08_full.rec,  Reconstruction (Pixel Size 1.25 nm), 1.2 GB  
        
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        Rp4Oct07_08.rec,  Reconstruction (Pixel Size 1.25 nm), 252.0 MB  
        
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        Rp4Oct07_08fil1.rec,  Reconstruction (Pixel Size 1.25 nm) 1x median filtered, 252.0 MB  
        
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        Rp4Oct07_08fil2.rec,  Reconstruction (Pixel Size 1.25 nm) 2x median filtered, 252.0 MB  
        
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        Rp4Oct07_08fil3.rec,  Reconstruction (Pixel Size 1.25 nm) 3x median filtered, 252.0 MB  
        
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        Rp4Oct07_08fil4.rec,  Reconstruction (Pixel Size 1.25 nm) 4x median filtered, 252.0 MB  
        
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