cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.2bamyw
title: Occurrence Download

description: A dataset containing 430 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((6.925 52.825,6.975 52.825,6.975 52.875,6.925 52.875,6.925 52.825))",
 "DatasetKey is Observation.org, Nature data from around the World",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 430 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0307987-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3pxm3a
title: Occurrence Download

description: A dataset containing 94 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is InvBasa UN"
 ]
}

 The dataset includes 94 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0289489-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25582/data.2023-05.2817897/2367993
title: Data for EMSL Project 51287 from May 2023
DOI: 10.15468/dl.z2k1fy
title: Occurrence Download

description: A dataset containing 29 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "BasisOfRecord is Human Observation",
 "BasisOfRecord is Living Specimen",
 "BasisOfRecord is Observation"
 ]
 },
 "HasCoordinate is true",
 "TaxonKey is Caesalpinia spinosa (Molina) Kuntze",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 29 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010653-180824113759888/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25582/data.2023-05.2817071/2367994
title: Data for EMSL Project 51287 from May 2023
DOI: 10.25582/data.2023-05.2817100/2367995
title: Data for EMSL Project 51287 from May 2023
DOI: 10.5281/zenodo.15140134
title: Table 4 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 4 Total glucosinolate (GSL) levels and profiles of GSLs derived from aromatic amino acids in Planodes virginica, Nasturtium officinale and Rorippa sylvestris. a
NTotalSDPEBAREBAR4hEBARIM4moIM10540 S40 R139 R4348μmol/g dry wt.Planodes virginica (Acc. 1, Kentucky)Roots36.81.80.060.054.3n.d.1.2n.d.Stems38.43.30.0010.096.5n.d.0.62n.d.Leaves31540.0010.1813tr.1.4n.d.Siliques31450.0020.1711n.d.0.65n.d.Seeds166–0.140.73440.250.11n.d.Planodes virginica (Acc. 2, Ohio)Leaves52430.0020.3417(0.03)b3.7n.d.Nasturtium officinale (two accessions, ‘Wild Ohio’ and ‘Johnny’ s Select Seeds’)Seeds (wild Ohio)147–310.01*0.07n.d.tr.*tr.*Seeds (Johnny’ s)178–580.01*0.18n.d.tr.*tr.*Rorippa sylvestrisSeeds27126n.d.n.d.n.d.n.d.n.d.n.d.
N is number of replicates, SD is standard deviation, other data are mean levels.
a n-HomoMet-derived and other aliphatic GSLs as specified in Table 5. Searched for but not detected (all three species): The typical Barbarea seed isoferuloyl derivatives (6’iF PE, 6’iF IM, 6’iF BAR, 6’iF EBAR), as well as hypothetic isoferuloyl and benzoyl derivatives of other dominating GSLs. Searched for but not detected in N. officinale: BZ, 4hBZ, 4hEBAR.Searched for but not detected in P. virginica and R. sylvestris: all substituted IMs: 4hIM, 1moIM, 4moIM, 1,4moIM. Searched for and not detected in P. virginica: BZ, 4hBAR, hydroxy-methoxy derivatives 4moEBAR and 3/4 hmoEBAR (“ x1 ”). GSL data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
b Confirmed by UHPLC-QToF (Supplementary Fig. S4) and quantified tentatively based on N 3 samples.
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 9 in Phytochemistry (112658) 185 on page 9, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140137
title: Table 5 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 5 Profiles of glucosinolates (GSLs) derived from aliphatic amino acids in various organs of Planodes virginica, Nasturtium officinale and Rorippa sylvestris. a
Methylsulfinylalkyls (MeSOalkyls)MeSO2alkylsMethylthioalkyls (MeSalkyls)n = 4n = 5n = 6n = 7n = 8n = 9* n = 8n = 5*n = 6*n = 7n = 8n = 9*647267666968 *8094 *88 *8792[89] *μmol/g dry wt.Planodes virginica (Acc. 1, Kentucky)Rootstr.0.040.020.280.790.050.01*n.d.tr.*0.020.02n.d.Stemstr.0.010.040.280.780.050.01*n.d.n.d.n.d.n.d.n.d.Leavestr.0.010.020.180.480.050.01*n.d.n.d.n.d.n.d.n.d.Siliquestr.0.020.040.431.20.090.03*n.d.n.d.n.d.n.d.n.d.Seedstr.b0.10b0.384.8130.480.05*tr.tr.*0.481.3tr.*Planodes virginica (Acc. 2, Ohio)Leavestr.b0.040.201.21.70.110.02*n.d.n.d.n.d.n.d.n.d.Nasturtium officinaleSeeds (OH)tr.*0.020.093.93.90.040.17n.d.0.84.82.0n.d.Seeds (John)tr.*0.02b0.124.95.50.120.12n.d.1.55.12.5n.d.Rorippa sylvestrisSeedsn.d.n.d.n.d.0.06563.21.1n.d.n.d.0.02*100.05*
a Data (mean levels) and N from the same collection as in Table 4. In addition to the listed GSLs, an unidentified isomer of 2h3mPe (149), with the desulfo derivative closely eluting yet chromatographically distinct from d 149, was detected at ca. 0.01 μmol/g dry wt. in P. virginica (both accessions), and unidentified isomers of 2mBu and 3mPe at trace level were detected in N. officinale (Section 2.2.4.). Searched for but not detected in any of the three species: 10mSOd, 10mSd, Pren, Buen, the typical A. thaliana GSLs 3hPr, 4hBu, 3BzOp, 4BzOb, 5BzOp, as well as BCAA-derived 1mEt, 1mPr, 2mPr, 2mBu, 2h2mBu, 3mPe, 3hmPe, 2h3mPe. GSL numbers labelled with an asterisk indicate tentative identification due to lack of an authentic standard, while individual data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
b Identity confirmed by comp. witn an authentic std. using UHPLC-QToF (Supplementary Fig. S4F-G).
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 9 in Phytochemistry (112658) 185 on page 9, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140136
title: Table 5 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 5 Profiles of glucosinolates (GSLs) derived from aliphatic amino acids in various organs of Planodes virginica, Nasturtium officinale and Rorippa sylvestris. a
Methylsulfinylalkyls (MeSOalkyls)MeSO2alkylsMethylthioalkyls (MeSalkyls)n = 4n = 5n = 6n = 7n = 8n = 9* n = 8n = 5*n = 6*n = 7n = 8n = 9*647267666968 *8094 *88 *8792[89] *μmol/g dry wt.Planodes virginica (Acc. 1, Kentucky)Rootstr.0.040.020.280.790.050.01*n.d.tr.*0.020.02n.d.Stemstr.0.010.040.280.780.050.01*n.d.n.d.n.d.n.d.n.d.Leavestr.0.010.020.180.480.050.01*n.d.n.d.n.d.n.d.n.d.Siliquestr.0.020.040.431.20.090.03*n.d.n.d.n.d.n.d.n.d.Seedstr.b0.10b0.384.8130.480.05*tr.tr.*0.481.3tr.*Planodes virginica (Acc. 2, Ohio)Leavestr.b0.040.201.21.70.110.02*n.d.n.d.n.d.n.d.n.d.Nasturtium officinaleSeeds (OH)tr.*0.020.093.93.90.040.17n.d.0.84.82.0n.d.Seeds (John)tr.*0.02b0.124.95.50.120.12n.d.1.55.12.5n.d.Rorippa sylvestrisSeedsn.d.n.d.n.d.0.06563.21.1n.d.n.d.0.02*100.05*
a Data (mean levels) and N from the same collection as in Table 4. In addition to the listed GSLs, an unidentified isomer of 2h3mPe (149), with the desulfo derivative closely eluting yet chromatographically distinct from d 149, was detected at ca. 0.01 μmol/g dry wt. in P. virginica (both accessions), and unidentified isomers of 2mBu and 3mPe at trace level were detected in N. officinale (Section 2.2.4.). Searched for but not detected in any of the three species: 10mSOd, 10mSd, Pren, Buen, the typical A. thaliana GSLs 3hPr, 4hBu, 3BzOp, 4BzOb, 5BzOp, as well as BCAA-derived 1mEt, 1mPr, 2mPr, 2mBu, 2h2mBu, 3mPe, 3hmPe, 2h3mPe. GSL numbers labelled with an asterisk indicate tentative identification due to lack of an authentic standard, while individual data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
b Identity confirmed by comp. witn an authentic std. using UHPLC-QToF (Supplementary Fig. S4F-G).
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 9 in Phytochemistry (112658) 185 on page 9, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140138
title: Table 6 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 6 Total glucosinolate (GSL) levels and profiles of GSLs derived from aromatic amino acids in two accessions of Cardamine hirsuta. a
NTotalSDBZPEIM1moIM4hIM4moIM1110543472848Organμmol/g dry wt.Accession 1 (Skyggeh¨oj) on peat-based soil among cultivated Rhododendron spp.Roots1b26–20n.d.0.880.01*0.060.51Leaves4c4912190.04*0.06n.d.0.310.19Siliques1d16–8.30.02*n.d.n.d.n.d.n.d.Accession 2 (Villa Rolighed) on loamy, clayey soil.Roots1b41–291.30.920.990.02*0.41Leaves4 c506190.52.50.020.01*0.07Siliques1d27–6.70.30.78n.d.n.d.n.d.
N is number of replicates, SD is standard deviation, other data are mean levels.
a n-HomoMet-derived and other aliphatic GSLs as specified in Table 7. Searched for but not detected: BAR, EBAR, 1,4moIM. Pooled stems (N = 1 + 1) showed a similar profile as other plant parts (Acc 1: mean 11 μmol/g dry wt. Acc 2 mean 8.5 μmol/g dry wt.)., except that a possible trace of 4hBZ (23) was detected in one sample of stems (Accession 1). GSL data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
b Pooled roots from the population.
c Mean for one batch of pooled cauline leaves, one batch of pooled rosette leaves and two samples of cauline leaves from individual plants. Samples of individual plants showed the same qualitative profile as seen in pools, and rosette leaves and cauline leaves showed similar profiles.
d Pooled siliques from the population.
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 11 in Phytochemistry (112658) 185 on page 11, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140139
title: Table 6 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 6 Total glucosinolate (GSL) levels and profiles of GSLs derived from aromatic amino acids in two accessions of Cardamine hirsuta. a
NTotalSDBZPEIM1moIM4hIM4moIM1110543472848Organμmol/g dry wt.Accession 1 (Skyggeh¨oj) on peat-based soil among cultivated Rhododendron spp.Roots1b26–20n.d.0.880.01*0.060.51Leaves4c4912190.04*0.06n.d.0.310.19Siliques1d16–8.30.02*n.d.n.d.n.d.n.d.Accession 2 (Villa Rolighed) on loamy, clayey soil.Roots1b41–291.30.920.990.02*0.41Leaves4 c506190.52.50.020.01*0.07Siliques1d27–6.70.30.78n.d.n.d.n.d.
N is number of replicates, SD is standard deviation, other data are mean levels.
a n-HomoMet-derived and other aliphatic GSLs as specified in Table 7. Searched for but not detected: BAR, EBAR, 1,4moIM. Pooled stems (N = 1 + 1) showed a similar profile as other plant parts (Acc 1: mean 11 μmol/g dry wt. Acc 2 mean 8.5 μmol/g dry wt.)., except that a possible trace of 4hBZ (23) was detected in one sample of stems (Accession 1). GSL data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
b Pooled roots from the population.
c Mean for one batch of pooled cauline leaves, one batch of pooled rosette leaves and two samples of cauline leaves from individual plants. Samples of individual plants showed the same qualitative profile as seen in pools, and rosette leaves and cauline leaves showed similar profiles.
d Pooled siliques from the population.
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 11 in Phytochemistry (112658) 185 on page 11, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140141
title: Table 7 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 7 Profiles of glucosinolates derived from aliphatic amino acids in two accessions of Cardamine hirsuta. a
4mSObBuen5mSOpPeenR 2hBuenS 2hBuen8mSOoGlobal No. 641272 *10124 R24 S*69Organμmol/g dry wt.Accession 1 (Skyggeh¨oj) on peat-based soil among cultivated Rhododendron spp.Roots 0.430.6n.d.n.d.2.5tr.*0.58Leaves 0.756.5n.d.n.d.21tr.*0.26Siliques 0.090.5n.d.n.d.6.8tr.*0.14Accession 2 (Villa Rolighed)) on loamy, clayey soilRoots 0.283.40.63.50.030.02*0.08Leaves 0.29141.611.70.060.03*0.29Siliques 0.06120.27.50.080.02*0.12
a Data (mean levels) and N from the same collection as in Table 6. Pooled stems (N 1 1) showed a similar profile as other plant parts (Acc 1: mean 9.4 μmol/g dry = + wt. Acc 2 mean 14 μmol/g dry wt.). Searched for but not detected: 1mEt, 1mPr, 2mPr, 2mBu, 2h2mBu, 3mPe, 2h3mPe, 3hmPe, Pren, 4mSb, 8mSO 2 o. GSL numbers labelled with an asterisk indicate tentative identification due to lack of an authentic standard (no auth. std. of 72 was available when this sample was run in 2012), while individual data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 11 in Phytochemistry (112658) 185 on page 11, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140140
title: Table 7 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 7 Profiles of glucosinolates derived from aliphatic amino acids in two accessions of Cardamine hirsuta. a
4mSObBuen5mSOpPeenR 2hBuenS 2hBuen8mSOoGlobal No. 641272 *10124 R24 S*69Organμmol/g dry wt.Accession 1 (Skyggeh¨oj) on peat-based soil among cultivated Rhododendron spp.Roots 0.430.6n.d.n.d.2.5tr.*0.58Leaves 0.756.5n.d.n.d.21tr.*0.26Siliques 0.090.5n.d.n.d.6.8tr.*0.14Accession 2 (Villa Rolighed)) on loamy, clayey soilRoots 0.283.40.63.50.030.02*0.08Leaves 0.29141.611.70.060.03*0.29Siliques 0.06120.27.50.080.02*0.12
a Data (mean levels) and N from the same collection as in Table 6. Pooled stems (N 1 1) showed a similar profile as other plant parts (Acc 1: mean 9.4 μmol/g dry = + wt. Acc 2 mean 14 μmol/g dry wt.). Searched for but not detected: 1mEt, 1mPr, 2mPr, 2mBu, 2h2mBu, 3mPe, 2h3mPe, 3hmPe, Pren, 4mSb, 8mSO 2 o. GSL numbers labelled with an asterisk indicate tentative identification due to lack of an authentic standard (no auth. std. of 72 was available when this sample was run in 2012), while individual data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 11 in Phytochemistry (112658) 185 on page 11, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140142
title: Table 8 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 8 Varying single nucleotide positions among ITS sequence variants within Barbarea vulgaris including newly obtained and publicly available sequences by mid-2019 (Fig. 8). In total seven groups of sequence types were found, among which group 2 and 3 differ from the G-type only by additional sequence ambiguities not indicated here. Eight sites are distinguishing between P- and G-types as indicated in bold.
Sequence group No. aNumber of accessionsType or subspeciesPosition in alignment b8183181251452474565586617631P-type cCAGCCATTC121G-type cTTATCGCCT79“ rivularis ” cTTATTGCTT43(PxG?)dCTAY eCGTTT52–CTGTTGCTT21–TTATCGCCT31–TTATCGCCT
a See Supplementary Table S1 and Fig. 8. GenBank accession codes for the newly acquired sequences are: Group 6 (P-type), MN508440; Group 1 (G-type and ssp. vulgaris), MN508439.
b Refer to the alignment in Supplementary Table S2.
c The designations of P- and G-type represents the majority of G-type and P-type accessions in Supplementary Table S1. Sequences from material identified as ssp. rivularis or ssp. vulgaris tended to cluster in sequence group 7, although a few ssp. vulgaris sequences were also found in sequence group 1.
d Preliminary interpretation of the intermediacy of the sequence: DNA sequence information and phylogenetic position in the network of accessions from group 4 might indicate that these accessions represent hybrids between the two clearly separated clades found within B. vulgaris according to the SplitTree analysis.
e Y: sequence ambiguity, indicating multiple ITS copies within a single individual with differing nucleotides (C or T).
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 13 in Phytochemistry (112658) 185 on page 13, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676
DOI: 10.5281/zenodo.15140143
title: Table 8 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

description: Table 8 Varying single nucleotide positions among ITS sequence variants within Barbarea vulgaris including newly obtained and publicly available sequences by mid-2019 (Fig. 8). In total seven groups of sequence types were found, among which group 2 and 3 differ from the G-type only by additional sequence ambiguities not indicated here. Eight sites are distinguishing between P- and G-types as indicated in bold.
Sequence group No. aNumber of accessionsType or subspeciesPosition in alignment b8183181251452474565586617631P-type cCAGCCATTC121G-type cTTATCGCCT79“ rivularis ” cTTATTGCTT43(PxG?)dCTAY eCGTTT52–CTGTTGCTT21–TTATCGCCT31–TTATCGCCT
a See Supplementary Table S1 and Fig. 8. GenBank accession codes for the newly acquired sequences are: Group 6 (P-type), MN508440; Group 1 (G-type and ssp. vulgaris), MN508439.
b Refer to the alignment in Supplementary Table S2.
c The designations of P- and G-type represents the majority of G-type and P-type accessions in Supplementary Table S1. Sequences from material identified as ssp. rivularis or ssp. vulgaris tended to cluster in sequence group 7, although a few ssp. vulgaris sequences were also found in sequence group 1.
d Preliminary interpretation of the intermediacy of the sequence: DNA sequence information and phylogenetic position in the network of accessions from group 4 might indicate that these accessions represent hybrids between the two clearly separated clades found within B. vulgaris according to the SplitTree analysis.
e Y: sequence ambiguity, indicating multiple ITS copies within a single individual with differing nucleotides (C or T).
description: Published as part of Agerbirk, Niels, Hansen, Cecilie Cetti, Olsen, Carl Erik, Kiefer, Christiane, Hauser, Thure P., Christensen, Stina, Jensen, Karen R., Ørgaard, Marian, Pattison, David I., Lange, Conny Bruun Asmussen, Cipollini, Don & Koch, Marcus A., 2021, Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates, pp. 13 in Phytochemistry (112658) 185 on page 13, DOI: 10.1016/j.phytochem.2021.112658, http://zenodo.org/record/8259676