cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.bq570/5
title: Data_siring success and seed production_experiment 2

description: Data on siring success of a focal pollen donor and seed production in Collinsia heterophylla following two-donor crosses involving a focal pollen donor and a standard competing pollen donor on a set of recipient plants.  Pollen from the focal pollen donor was applied at floral developmental stage 1 and pollen from the standard competitor was applied at floral developmental stage 2, i.e. one day later. Siring success estimated as proportion seeds sired by the donors using microsatellite markers in up to 20 seeds from four replicate crosses using the same focal and standard pollen donors, and the same recipient. Seed production estimated as number of seeds per capsule as a mean of the four replicate crosses.
DOI: 10.5061/dryad.bs41q/1
title: Phenotypic matrices with size variation

description: Pearson product-moment correlation matrices of all toad species for the 21 linear distances.
DOI: 10.5061/dryad.f8c4f/1
title: MicrosatPloidyData

description: Table of individuals with ploidy information	
Individual_ID	Population ID with individual numbers. See Appendix A for more information
Life.history	Morphological identification of life-history state
msatploidy	Microsatellite-based identification of ploidy
mlg.id	the multilocus ID for each diploid per population
pgen	probability that the thallus was a unique multi-locus genotype
psex	probability that the thallus represents a separate sexual reproductive event (i.e. a separate genet)
DOI: 10.5061/dryad.f8c4f/2
title: PhenotypeData

description: Phenotype dataset	
assay	Assay type (40deg, minus20deg, salinity)
ind.type	field vs common garden
ind	individual ID
trt	levels within each assay type
SBS	standardized bleaching code (-2 to 2)
DOI: 10.5061/dryad.hg971/1
title: Effect of abiotic conditions_ floral measurements

description: Effect of abiotic conditions on floral traits of Lithophragma cymbalaria. Three light levels were manipulated for two soil and water conditions.
DOI: 10.5061/dryad.hg971/2
title: Effect of abiotic conditions_number of flowers

description: Effect of abiotic conditions on number of flowers produced per plant. Three light levels were tested for two soil and water conditions.
DOI: 10.5061/dryad.hg971/3
title: Greya morphological measurements

description: Morphological measurements of Greya obscura and G. politella moths collected from Lithophragma flowers
DOI: 10.5061/dryad.hg971/4
title: Lithophragma floral measurements

description: Morphological measurements of Lithophragma floral traits.
DOI: 10.5061/dryad.m7fg0/1
title: Fruit production and plant cover for two Vaccinium species

description: Fruit production and plant cover for Vaccinium myrtillus and Vaccinium vitis-idaea collected in experimental plots. See readme file for more info.
DOI: 10.5061/dryad.p8s64/1
title: Data from: Venus flytrap rarely traps its pollinators

description: Includes: (1) Data used to assess difference in probability of pollen-carrying across orders (Fig. 1a). (2) Subsample counts of number of pollen grains in 5 fields of view on fuchsin-jelly slides, for up to 10 specimens each of the 10 most common flower-visitor taxa. (3) Data used to assess niche overlap and generate network diagram (Fig. 2)
DOI: 10.5061/dryad.pt29h/1
title: Cuticular hydrocarbon data of Temnothorax longispinosus and T. ambiguus

description: The file contains a table with information on experimental treatments, behavioural castes, and all CHC traits analysed. The second table contains information on the detailed CHC composition of each sample.
DOI: 10.5061/dryad.q755t/1
title: Vital rate and internode data

description: This data file contains annual vital rate data (stem length growth, fruit production, survival and leaf production) of 830 individuals of the understorey palm Chamaedorea elegans, collected in a 0.7 ha plot in Chiapas, Mexico, during the period November 2012 - November 2015. A 2/3 defoliation treatment was repeatedly applied to half of the individuals. The data file also contains measurements of the lengths of all internodes of all individuals.
DOI: 10.5061/dryad.q96g6.2/1.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 01_01

description: Embryo 01 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/10.2
title: Live widefield JF635-HaloTag-Ubx late stage 6 embryo

description: Wide field imaging in the anterior region of a live late stage 6 Drosophila melanogaster embryo expressing HaloTag-Ubx. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
DOI: 10.5061/dryad.q96g6.2/2.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 02_01

description: Embryo 02 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/3.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 02_02

description: Embryo 02 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/4.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 03_01

description: Embryo 03 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/5.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 03_02

description: Embryo 03 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/6.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 04_01

description: Embryo 04 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/7.2
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 04_02

description: Embryo 04 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/8.2
title: Live widefield JF635-HaloTag-NLS stage 5 embryo

description: Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-NLS. The HaloTag-NLS protein is unstable and degraded after translation. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
DOI: 10.5061/dryad.qq435/1
title: Raw behavioural data

description: Raw data for both the Natural vs Unnatural experiment (testing the response of Australian sea lion females to pup models with natural vs unnatural pelage colour pattern) and Age Classes experiment (testing the ability of Australian sea lion females to distinguish pup models based on age-class-specific pelage patterns)
DOI: 10.5061/dryad.s1n6p/1
title: Eggshell data, including pigment concentrations and bacteria community composition
DOI: 10.5061/dryad.v3t77/1
title: Female Uta stansburiana egg and wound leucine allocation data

description: Each row is one individual female Uta stansburiana. For columns, see README
DOI: 10.17632/2fmxwsbpvm.1
title: Data for: Glucocorticoid Receptor Potentiation Differentially Affects Various Behavioural Paradigms and Central Insulin Level in Wistar Rats
DOI: 10.5061/dryad.03v7p/10
title: Class Population: header file
DOI: 10.5061/dryad.03v7p/11
title: Class Population: cpp file
DOI: 10.5061/dryad.03v7p/12
title: Class Individual: header file
DOI: 10.5061/dryad.03v7p/13
title: Class Individual: cpp file
DOI: 10.5061/dryad.0k8c4/1
title: MedicalAdmissions

description: De-identified data on medical inpatient admissions (age rounded to within 10 years).
DOI: 10.5061/dryad.13173/1
title: oikos_dryad_wiggins_OIK-04766R1

description: data associate with juvenile jumping spiders fed live prey that varied in nutrients alter growth rates and sizes over four months.
DOI: 10.5061/dryad.1rb14/1
title: Prevalence, associated factors, awareness, treatment and control of hypertension in Surkhet, Mid-western region of Nepal
DOI: 10.5061/dryad.2d8b5/1
title: Stress calcification phenotypes and sampling populations

description: Text file containing standardized change in buoyant weight (calcification rate) as well as sampling population origin for each mussel. Mussels that did not survive to final buoyant weight measurement are not included.
DOI: 10.5061/dryad.2hj75/1
title: polymorphic_snps_pbl_cru_va

description: VCF file of the polymorphic loci among 30 individuals from three Galápagos giant tortoise species. Data are derived from ddRAD sequencing. PBL corresponds to Chelonoidis becki, CRU corresponds to C. porteri, and VA corresponds to C. vandenburghi.
DOI: 10.5061/dryad.36q03/1
title: data
DOI: 10.5061/dryad.49g85/1
title: Appendix_2

description: DNA sequence matrices on which phylogenetic analyses were based
DOI: 10.5061/dryad.49g85/2
title: Collection coordinates

description: Latitude/longitude coordinates for collections of Cremastosperma and Mosannona (Annonaceae) species analysed for niche modelling.
DOI: 10.5061/dryad.49g85/3
title: Environmental layers

description: Environmental layers used for niche modelling of Cremastosperma and Mosannona species.
DOI: 10.5061/dryad.4s1gg/1
title: Pika samples SNP file

description: The complete SNP file for all the Pika samples we used.
DOI: 10.5061/dryad.4s1gg/2
title: pika sampling location

description: sampling location of all the samples
DOI: 10.5061/dryad.4s1gg/3
title: pika vcf

description: vcf file of all the pika samples
DOI: 10.5061/dryad.53512/1
title: cell trajectories data

description: Cell trajectories for all experiments reported in this paper are provided
DOI: 10.5061/dryad.53512/2
title: summary directedness angle

description: A summary of directedness and migration angle for all experiments is provided
DOI: 10.5061/dryad.5d926/10
title: Line 1, H9p37 (short), Differentiated, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/11
title: Line 1, H9p37 (short), Undifferentiated, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/13
title: Line 1, H9p81, Undifferentiated, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/14
title: Line 1, IMR90 Fibroblast, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/5
title: Line 1, a Fibroblast cell line established from an individual with fragile X syndrome
DOI: 10.5061/dryad.5d926/6
title: Line 1, Fragile X iPS1, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/7
title: Line 1, Fragile X iPS2, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/8
title: Line 1, H9p37 (long), Differentiated, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5d926/9
title: Line 1, H9p37 (long), Undifferentiated, double-stranded DNA methylation patterns
DOI: 10.5061/dryad.5g0rg/1
title: Individual histories for moose at Vega 1984-2012

description: Individual reproductive and survival histories for female moose at Vega 1984-2012. Numbers according to states: 
1: Produced 0 calves, 2: Produced 1 calf, 3: Produced 2 calves, 4: Hunted, and 5: Natural death.
DOI: 10.5061/dryad.7c4s5/10
title: Table S10

description: Significantly enriched canonical pathways from IPA ranked by p-value or z-score relative to FR
DOI: 10.5061/dryad.7c4s5/11
title: Table S11

description: Linear regression analyses comparing –log10 of pathway p-values and upstream regulator z-scores from the frozen (FR) sample versus predictions from respective preservation procedures
DOI: 10.5061/dryad.7c4s5/12
title: Table S12

description: Ingenuity Pathway Analysis upstream regulator comparison ranked relative to FR p-value
DOI: 10.5061/dryad.7c4s5/13
title: Table S13

description: Casual network analysis comparing -log10(p-values) across preservation groups
DOI: 10.5061/dryad.7c4s5/14
title: Figure S1

description: Correlation analysis by log fold change of the differentially expressed genes found within each preservation condition and corresponding frozen group. Abbreviations: FR - frozen, OH - ethanol, 18F – 18hr formalin, 3F - 3m formalin, DTAE - Demod Tris-Acetate EDTA, DQ - Demod 18hr Qiagen, DP - Demod 18hr PureLink, and NoD - Control no catalyst
DOI: 10.5061/dryad.7c4s5/15
title: Figure S2

description: Plot of –log10 of pathway p-values from the frozen (FR) sample versus pathway predictions from the other preservation procedures. Best fit linear regression line is shown and line expression is shown in the upper right side of each panel. Panels are: A) FR versus OH; B) FR versus 18F; C) FR versus DTAE; D) FR versus DP; E) FR versus DQ; F) FR versus NoD; G) FR versus 3F. Abbreviations: FR - frozen, OH - ethanol, 18F – 18hr formalin, 3F - 3m formalin, DTAE - Demod Tris-Acetate EDTA, DQ - Demod 18hr Qiagen, DP - Demod 18hr PureLink, and NoD - Control no catalyst
DOI: 10.5061/dryad.7c4s5/16
title: Figure S3

description: Plot of Upstream Regulator activation z-scores from the frozen (FR) sample versus Upstream Regulator activation z-scores from the other isolation procedures. Best fit linear regression line is shown and line expression is shown in the upper right side of each panel. Panels are: A) FR versus DP; B) FR versus 18F; C) FR versus DTAE; D) FR versus OH; E) FR versus DQ; F) FR versus 3F; G) FR versus NoD. Abbreviations: FR - frozen, OH - ethanol, 18F – 18hr formalin, 3F - 3m formalin, DTAE - Demod Tris-Acetate EDTA, DQ - Demod 18hr Qiagen, DP - Demod 18hr PureLink, and NoD - Control no catalyst
DOI: 10.5061/dryad.7c4s5/9
title: Table S9

description: TMM normalized and raw biomarker (Cyp2b10, Cyp3a11) and housekeeping gene 
(Gapdh) count summaries
DOI: 10.5061/dryad.9sk4v/10
title: SI 10 - Position of study species within Passerine phylogeny
DOI: 10.5061/dryad.9sk4v/11
title: SI 11 - Analysis of spatial auto-correlation in assemblage/spatial models
DOI: 10.5061/dryad.9sk4v/5
title: SI 5 - Manually curated metrics from Xeno-Canto

description: Manually curated birdsong metrics used for analysis (see manuscript for details)
DOI: 10.5061/dryad.9sk4v/6
title: SI 6 - Xeno-Canto recording meta-data

description: Meta-data describing each recording downloaded from Xeno-Canto (downloaded using script in SI 3).
DOI: 10.5061/dryad.9sk4v/7
title: SI 7 - Additional spatial/assemblage analyses

description: Additional spatial/assemblage model results, as described in the manuscript. The first sheet describes the models in greater detail; subsequent sheets give the model outputs
DOI: 10.5061/dryad.9sk4v/8
title: SI 8 - Additional comparative/PGLS analyses

description: Additional comparative/PGLS model results, as described in the manuscript. The first sheet describes the models in greater detail; subsequent sheets give the model outputs
DOI: 10.5061/dryad.9sk4v/9
title: SI 9 - Additional plots of birdsong metrics against environmental variables
DOI: 10.5061/dryad.bs41q/2
title: List of covariance matrices of all toad species

description: List of residual covariance matrices for the 21 linear distances extracted from toad skull. The matrices were constructed using residuals of multivariate linear models controlling for sex and locality. This list can be read in R using load('cov.list_data'). To construct correlation matrices, use lapply(cov.list, cov2cor).
DOI: 10.5061/dryad.bs41q/3
title: Definition of each developmental, hormonal and functional unit

description: Data frame describing which distances belong to each developmental, hormonal or functional unit. 1 for distances that belong to the unit and 0 for distances that do not belong to the unit. May be used as the argument 'modularity.hypot' in the function 'TestModularity' of the 'evolqg' R package.
DOI: 10.5061/dryad.bs41q/4
title: Modularity models for P-matrices with size variation

description: Data frame describing the modularity models used to test P-matrices with size variation. May be used as argument 'mod' in the 'EMMLi' function in R.
DOI: 10.5061/dryad.bs41q/5
title: Modularity models for P-matrices without allometry

description: Description of modularity models used to test P-matrices without allometric size variation. May be used as argument 'mod' in 'EMMLi' R function.
DOI: 10.5061/dryad.bs41q/6
title: Modularity models for P-matrices without isometry

description: Description of modularity models used to test P-matrices without isometric size variation. May be used as argument 'mod' in 'EMMLi' R function.
DOI: 10.5061/dryad.cv20d/1
title: Salmon_AllRivers_Genepop

description: Genepop file of all European and North American rivers with 1773 loci.
DOI: 10.5061/dryad.cv20d/2
title: Genepop files of clinal loci

description: Two genepop files with clinal loci from North American and European rivers.
DOI: 10.5061/dryad.cv20d/3
title: Environmental Data

description: Environmental data used in our study for climate associations with genetic clustering coefficients.
DOI: 10.5061/dryad.cv20d/4
title: R_scripts

description: R scripts for selecting clinal loci based on our criteria and replicating our analyses looking at structure and climate associations.
DOI: 10.5061/dryad.ds946/1
title: Tarling_SCQ_RawData

description: Safety Climate raw data and demographic data
DOI: 10.5061/dryad.fq4h3/1
title: 9cp+11nr_201_ME

description: The matrix used for phylogeny reconstrcution
DOI: 10.5061/dryad.fq4h3/2
title: CenozoicTemperature from 65 Ma
DOI: 10.5061/dryad.kq23s/1
title: ROUTE_proteinuria
DOI: 10.5061/dryad.m6q7c/1
title: microsatellites dataset

description: The genotyped organism is haploid fungus Venturia inaequalis. Data are obtained for 20 fungus populations, each comprising 25-32 individuals. First two rows contain descriptions of data. Each subsequent row contains allele data, scored for 11 microsatellite loci, for one individual. Missing data are coded as: -9.
DOI: 10.5061/dryad.m6q7c/2
title: sampling locations

description: File contains names of the 20 genotyped populations (of fungus Venturia inaequalis) and the approximate GPS position of the orchard, in which they were sampled.
DOI: 10.5061/dryad.m7fg0/2
title: Species richness of multiple species groups

description: Species richness of pollinators, saproxylic beetles, bryophytes, vascular plants and lichens. Collected in experimental plots. See readme file for more info.
DOI: 10.5061/dryad.m7fg0/3
title: R script to reproduce results

description: R script with comments to reproduce results in the associated article.
DOI: 10.5061/dryad.m7fg0/4
title: Plant community data

description: Plant community data (percent cover) from experimental plots. Ground living vascular plants, macrolichens and bryophytes (mosses and liverworts). Year 2000, 2003 and 2011. See README file for more information.
DOI: 10.5061/dryad.n821p/1
title: Data Uroderma heart rates

description: Heart rates and calculated energetic expenditure of free-ranging tent-making bats (Uroderma bilobatum) in Gamboa, Panama.
DOI: 10.5061/dryad.n821p/2
title: Data Uroderma respirometry

description: Energy consumption, heart rate, body temperature, and ambient temperature of tent-making bats (Uroderma bilobatum) measured in open-flow respirometry.
DOI: 10.5061/dryad.n821p/3
title: Data Uroderma breath

description: Carbon stable isotope enrichment of tent-making bats (Uroderma bilobatum) that were fed figs or agave nectar to measure metabolic incorporation rates and fat turnover.
DOI: 10.5061/dryad.n821p/4
title: Data Uroderma cortisol

description: Cortisol dynamics of female and male tent-making bats (Uroderma bilobatum) sampled at baseline and after 1 hour of restraint.
DOI: 10.5061/dryad.q80f6/5
title: Supplementary material
DOI: 10.5061/dryad.q96g6.1
title: Data from: Nuclear microenvironments modulate transcription from low-affinity enhancers
DOI: 10.5061/dryad.q96g6.1/1.1
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 01_01

description: Embryo 01 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.1/2.1
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 02_01

description: Embryo 02 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.1/3.1
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 02_02

description: Embryo 02 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.1/4.1
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 03_01

description: Embryo 03 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.1/5.1
title: Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 03_02

description: Embryo 03 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
DOI: 10.5061/dryad.q96g6.2/11.2
title: Live widefield JF635-HaloTag-Ubx stage 5 embryo

description: Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx. This Ubx is the wildtype protein. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
DOI: 10.5061/dryad.q96g6.2/9.2
title: Live widefield JF635-HaloTag-Ubx(R3A N51A) stage 5 embryo

description: Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx(R3A N51A). This Ubx is DNA-binding deficient. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.