cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647963
title: CIL:24565

description: A frame from the grouped movie of a Balamuthia mandrillaris ameba within a culture of monkey kidney cells. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs demonstrate the puncture of the plasma membrane of a monkey kidney cell. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Details of the preparation can be found in CIL:19325.
DOI: 10.6084/m9.figshare.647964.v1
title: CIL:24566

description: A frame from the grouped movie of a Balamuthia mandrillaris ameba within a culture of monkey kidney cells. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs demonstrate the puncture of the plasma membrane of a monkey kidney cell. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Details of the preparation can be found in CIL:19325.
DOI: 10.6084/m9.figshare.647961
title: CIL:24043

description: Starved wild-type Dictyostelium cells migrate toward the cathode (left). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) This is a control video for CIL#s 24044, 24045, 24046 of mutants, originally isolated as chemotaxis-deficient mutants. Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647962.v1
title: CIL:24564

description: A single frame from the movie (CIL:20154) that shows a large rounded monkey kidney cell being penetrated by a pseudopodium followed by the whole Balamuthia ameba. Detection of the ameba within, rather than on, or beneath the cell is indicated by the change in clarity of the ameba outside and then inside the cell's exterior membrane. The ameba with splayed pseudopodia had begun to enter in apparently a slit in the surface of the cell. The integrity of the cell is maintained and its size does not change appreciably. The portion of the ameba within the cell is less clear than that portion still outside, and as it continues movement within the cell that continues until only a small portion (tail) of the ameba remains outside. Photographed at 1200X. Additional photographs (grouped with this movie) show the movement of the ameba inside of the cell its movement deforms the rounded exterior membrane before the whole ameba emerges some 20 minutes later.
DOI: 10.6084/m9.figshare.647963.v1
title: CIL:24565

description: A frame from the grouped movie of a Balamuthia mandrillaris ameba within a culture of monkey kidney cells. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs demonstrate the puncture of the plasma membrane of a monkey kidney cell. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Details of the preparation can be found in CIL:19325.
DOI: 10.6084/m9.figshare.647958
title: CIL:23043

description: Plectin sidearms on intermediate filaments labeled with plectin immuno-gold (10 nm) in REF-52 cells with actin removed by gelsolin treatment. Untreated control appears as CIL: 234044. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.
DOI: 10.6084/m9.figshare.647960
title: CIL:23599

description: Spindle oscillation in wild-type cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmic microtubule along the cell cortex. These events require dynein-dynactin function since nip100delta mutants display frequent contacts between microtubule ends and the cell cortex, but no spindle movements or microtubule sliding events were observed (Movie S2, CIL# 23603). Movie plays at 50x real time and is Movie S1 in Proc Natl Acad Sci. 2009. 106: 5147-5152.
DOI: 10.6084/m9.figshare.647959
title: CIL:23044

description: Plectin sidearms and intermediate filaments labeled with plectin immuno-gold (10 nm) in control cells. Cells with a similar label, but with actin removed by gelsolin treatment appears as CIL: 23403. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon.performed after cell lysis as described (Svitkina et al., 1995). Image corresponds to Fig 1d in J Cell Biol. 1996 Nov;135(4):991-1007.
DOI: 10.6084/m9.figshare.647960.v1
title: CIL:23599

description: Spindle oscillation in wild-type cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmic microtubule along the cell cortex. These events require dynein-dynactin function since nip100delta mutants display frequent contacts between microtubule ends and the cell cortex, but no spindle movements or microtubule sliding events were observed (Movie S2, CIL# 23603). Movie plays at 50x real time and is Movie S1 in Proc Natl Acad Sci. 2009. 106: 5147-5152.
DOI: 10.6084/m9.figshare.647961.v1
title: CIL:24043

description: Starved wild-type Dictyostelium cells migrate toward the cathode (left). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) This is a control video for CIL#s 24044, 24045, 24046 of mutants, originally isolated as chemotaxis-deficient mutants. Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647955
title: CIL:20812

description: The CV membrane has collapsed into regularly spaced tubules oriented perpendicular to the microtubular ribbon in this ruptured cell. Some tubules and some nearby vesicles contain electron opaque material probably obtained from the mixture of the external medium and cytosolic contents as the CV expelled its content and medium had a chance to enter the CV by backflow. TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13115) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647956
title: CIL:21991

description: Didinium captures Paramecium. A moment after initial contact when toxicysts enter Paramecium. The strand of toxicysts can be seen between the two organisms, and Didinium will use the anchored toxicysts to reel in Paramecium to the awaiting proboscis which is opening to accept its prey. The opening of the proboscis might be seen better in the same pair taken at a slightly different angle (CIL:21993). Also showing metachronous waves of cilia within the two characteristic ciliary girdles of Didinium nasutum; the waves on Paramecium may be paralyzed due to the effects of the attached toxicysts. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 495X. Further details are available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270.
DOI: 10.6084/m9.figshare.647957
title: CIL:21996

description: The plasma membrane of Paramecium multimicronucleatum seen in a freeze fracture replica. The two rows of IMPs can be seen on the P-face of the plasma membrane that connects the plasma membrane to the underlying margins of alveolar sacs. A gap 20nm wide composed of only the plasma membrane connects these two ribbons. The spent vacuole membrane will fuse with the membrane over this gap. Further interpretation of this image can be seen at Figure 4 of Chapter 7 at the link below. TEM taken on 6/3/75 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12346) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647958.v1
title: CIL:23043

description: Plectin sidearms on intermediate filaments labeled with plectin immuno-gold (10 nm) in REF-52 cells with actin removed by gelsolin treatment. Untreated control appears as CIL: 234044. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.
DOI: 10.6084/m9.figshare.647959.v1
title: CIL:23044

description: Plectin sidearms and intermediate filaments labeled with plectin immuno-gold (10 nm) in control cells. Cells with a similar label, but with actin removed by gelsolin treatment appears as CIL: 23403. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon.performed after cell lysis as described (Svitkina et al., 1995). Image corresponds to Fig 1d in J Cell Biol. 1996 Nov;135(4):991-1007.
DOI: 10.6084/m9.figshare.647955.v1
title: CIL:20812

description: The CV membrane has collapsed into regularly spaced tubules oriented perpendicular to the microtubular ribbon in this ruptured cell. Some tubules and some nearby vesicles contain electron opaque material probably obtained from the mixture of the external medium and cytosolic contents as the CV expelled its content and medium had a chance to enter the CV by backflow. TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13115) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647956.v1
title: CIL:21991

description: Didinium captures Paramecium. A moment after initial contact when toxicysts enter Paramecium. The strand of toxicysts can be seen between the two organisms, and Didinium will use the anchored toxicysts to reel in Paramecium to the awaiting proboscis which is opening to accept its prey. The opening of the proboscis might be seen better in the same pair taken at a slightly different angle (CIL:21993). Also showing metachronous waves of cilia within the two characteristic ciliary girdles of Didinium nasutum; the waves on Paramecium may be paralyzed due to the effects of the attached toxicysts. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 495X. Further details are available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270.
DOI: 10.6084/m9.figshare.647957.v1
title: CIL:21996

description: The plasma membrane of Paramecium multimicronucleatum seen in a freeze fracture replica. The two rows of IMPs can be seen on the P-face of the plasma membrane that connects the plasma membrane to the underlying margins of alveolar sacs. A gap 20nm wide composed of only the plasma membrane connects these two ribbons. The spent vacuole membrane will fuse with the membrane over this gap. Further interpretation of this image can be seen at Figure 4 of Chapter 7 at the link below. TEM taken on 6/3/75 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12346) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647951
title: CIL:20096

description: Camera lucida sketches of the zebrafish embryo at selected stages. This image is from the hatching period at the 60 hour pec-fin stage. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.
DOI: 10.6084/m9.figshare.647953
title: CIL:20098

description: Camera lucida sketches of the zebrafish embryo at selected stages. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.
DOI: 10.6084/m9.figshare.647954
title: CIL:20153

description: An ameba, Balamuthia mandrillaris, within a culture of monkey kidney feeder cells. Balamuthia extends from its branched pseudopodia while its rope-like body curves over and around a nucleus of a kidney cell; additional branches extended both downward and upward into and between other cells. Those branches terminate with more finger-like pseudopodia projections. The density and the movement of the cytoplasmic granules delineate the ameba from its feeder cells that, by comparison, are quiescent with few scattered cytoplasmic granules and a large, rounded nucleus containing several dense nucleoli. The purpose of this movie is to indicate the identity of an ameba amongst other cells in the co-culture. It shows the expanse of the ameba and its simultaneous involvement with several cells. Observations of the amebae under such conditions show their movement and interactions as they feed in a dimension unlike that of the ameba identification in sectioned material obtained from cases of amebic encephalitis. Details of the preparation can be found in CIL:19337.
DOI: 10.6084/m9.figshare.647951.v1
title: CIL:20096

description: Camera lucida sketches of the zebrafish embryo at selected stages. This image is from the hatching period at the 60 hour pec-fin stage. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.
DOI: 10.6084/m9.figshare.647952
title: CIL:20097

description: Camera lucida sketches of the zebrafish embryo at selected stages. This image is from the hatching period at the 72 hour protruding mouth stage. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.
DOI: 10.6084/m9.figshare.647953.v1
title: CIL:20098

description: Camera lucida sketches of the zebrafish embryo at selected stages. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.
DOI: 10.6084/m9.figshare.647952.v1
title: CIL:20097

description: Camera lucida sketches of the zebrafish embryo at selected stages. This image is from the hatching period at the 72 hour protruding mouth stage. The animal pole is to the top for the early stages, and anterior is to the top later, except for the two animal polar (AP) views shown below their side view counterparts for germ-ring and shield gastrulas. Face views are shown during cleavage and blastula stages. After shield stage, the views are of the embryo's left side, but before the shield arises one cannot reliably ascertain which side is which. Pigmentation is omitted. Arrowheads indicate the early appearance of some key diagnostic features at the following stages: 1 k-cell: YSL nuclei. Dome: the doming yolk syncytium. Germ ring: germ ring. Shield: embryonic shield. 75%-epiboly: Brachet's cleft. 90%-epiboly: blastoderm margin closing over the yolk plug. Bud: polster. 3-somite: third somite. 6-somite: eye primordium (upper arrow), Kupffer's vesicle (lower). 10-somite: otic placode. 21-somite: lens primordium. Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). Prim-16: heart. High-pec: pectoral fin bud. Composite image CIL: 20098 is a low resolution image with a scale bar = 250 microns that shows the entire set of image. Images correspond to Fig 1 of Developmental Dynamics 203:253-310 (1995). This material is reproduced with permission of John Wiley & Sons, Inc. Copyright 1995 WILEY-LISS, INC.