DOI: 10.5061/dryad.x95x69pw1
title: Data from: Effect of the combination floral and diet resources on development of big-eyed bug Geocoris ochropterus (Fieber) (Hemiptera: Geocoridae) at different temperature

description: The big-eyed bug, Geocoris ochropterus Fieber, is a polyphagous predator.
 Although there have been many publications on the rearing of big-eyed bugs
 by using other insects, their ability to use flowers as a source of
 nutrition or habitat has not been previously reported. The diets included
 Zinnia elegans (A), 10% honey solution (B), ant pupae and Zinnia elegans
 (C), mealybug and Zinnia elegans (D) treatments at 10°C, 20°C, and 30°C.
 Significant differences in survival rates were observed among the diets
 when the nymphs reached the 1st and 2nd instars at 10°C. Significant
 differences in growth and development indicators of big-eyed were found
 among the treatments during the 2nd, 3rd, 4th, and 5th molts at 30°C.
 Additionally, body size and dry weight of adult G. ochropterus grown on
 different diets were measured at 20°C and 30°C. At 20°C, male body size
 parameters did not significantly differ among the diets, except for head
 width. At 30°C, body length and dry weight of males showed significant
 differences among the diets, while head width and dry weight of females
 also varied significantly among diets. The study indicates that the
 combination of floral resources and diet can affect the development of G.
 ochropterus at different temperatures.
description: Insects <em>Geocoris
 ochropterus</em> was collected in Cu Chi district, Ho Chi Minh city
 and reared in the laboratory (Chau <em>et al</em>., 2021,
 2023) using ant pupae <em>Oecophylla smaragdina </em>as food
 source to collect eggs for experiments. Diets
 resources The diets used ant pupae <em>Oecophylla
 smaragdina</em> (Hymenoptera: Formicidae) purchased from Minh Nhat
 fishing shop, Cu Chi district, Ho Chi Minh City store in Thu Dau Mot, Binh
 Duong province, Vietnam. Adult mealybugs <em>Planococcus
 citri</em> were reared and maintained on fresh pumpkins in the
 laboratory. Flowers of common zinnia (<em>Zinnia elegans</em>)
 were choose (Han et al., 2024) and grown from seeds sown in 50 x 50 cm
 polybags. The plants flowered prolifically and produced florets throughout
 the experiment. Honey was purchased from the supermarket (U Minh honey –
 Viethoney) and diluted with water. Experiment
 design The experiment was conducted in the XINGCHEN
 GZX-150B growth chamber from April to August 2023. Newly hatched
 first-instar <em>G. ochropterus</em> were separately provided
 with different diets, including: flower <em>Zinnia
 elegans</em> (n=25) (Treatment A); 10% honey solution (n=25)
 (Treatment B); ant pupae and <em>Zinnia elegans</em> (n=25)
 (Treatment C); adult mealybugs and <em>Zinnia elegans</em>
 (n=25) (Treatment D); and each instar was reared in a plastic jar (Bottom
 diameter is 85mm x height 150mm x top diameter is 117mm) containing a
 piece of cotton soaked with water (Figure 1). The hydroponic solution used
 in the experiment is Lavamix Hydroponic (Duc Thuan Trading &
 Service Company Limited, Vietnam).The food source was ant pupae
 supplemented at 50 g per day, mealybugs at 10 individuals per day, 10%
 honey solution at 1 ml per day, and the lowers were replaced every 4 days
 to ensure freshness. All treatments were conducted at
 constant temperatures of 10 ± 1°C, 20 ± 1°C, and 30 ± 1°C, with 70 ± 5%
 relative humidity and a photoperiod of 16 hours light and 8 hours dark in
 a growth chamber (RGX-150B, Xingchen, China) at the Laboratory of Animal
 Science, Faculty of Biotechnology, Ho Chi Minh City Open University,
 Vietnam.                             The development
 time of each stage and survival rate (from first instar to adult) were
 recorded. The nymphal survival rate was calculated as the number of
 individuals that molt at each larval stage divided by the total number of
 eggs hatched. Morphological measurements
 According to Chau et al. (2021), the body size of one-day-old
 adults was measured with the following parameters: head width (from the
 compound eye), body length (from the anterior tip of the head to the
 posterior tip of the abdomen), and forewing length (from the
 posterolateral edge of the forewing to the tip of the forewing),
 individually using a digital ruler (Mitutoyo Corporation No.
 99MAC005B).  Statistical analysis
 Development time and survival rate were analyzed by using two-way
 factorial ANOVA to assess the differences between temperature and food
 source groups, and also to test the interaction between these two factors
 with SPSS 22.0, followed by Tukey’s post-hoc test for multiple comparisons
 to compare treatment means. For the statistical
 comparison of body size and weight, the statistical method used was
 Dunnett's Two-Sided T-Test.
description: # Data from: Effect of the combination floral and diet resources on
 development of big-eyed bug Geocoris ochropterus (Fieber) (Hemiptera:
 Geocoridae) at different temperature
 [https://doi.org/10.5061/dryad.x95x69pw1](https://doi.org/10.5061/dryad.x95x69pw1) ## Description of the data and file structure ### Files and variables Data file showing number of days to molt, total number of days to survive, number of males and females, total number of eggs laid, and size and weight of big-eyed bugs across four different food sources at 10, 20, and 30 degrees Celsius, respectively. The cells with "-" indicate not applicable. ## Code/software Software for data analysis: SPSS 22.0