DOI: 10.5061/dryad.2rf10
title: Data from: Gene duplication and co-evolution of G1/S transcription factors specificity in fungi are essential for optimizing cell fitness

description: Transcriptional regulatory networks play a central role in optimizing cell
 survival. How DNA binding domains and cis-regulatory DNA binding sequences
 have co-evolved to allow the expansion of transcriptional networks and how
 this contributes to cellular fitness remains unclear. Here we
 experimentally explore how the complex G1/S transcriptional network
 evolved in the budding yeast Saccharomyces cerevisiae by examining
 different chimeric transcription factor (TF) complexes. Over 300 G1/S
 genes are regulated by either one of the two TF complexes, SBF and MBF,
 which bind to specific DNA binding sequences, SCB and MCB, respectively.
 Our data suggests that whilst SBF is the likely ancestral regulatory
 complex, the ancestral DNA binding element is more MCB-like. G1/S network
 expansion took place by both cis- and trans- co-evolutionary changes in
 closely related but distinct regulatory sequences. Replacement of the
 endogenous SBF DNA-binding domain (DBD) with that from more distantly
 related fungi leads to a contraction of the G1/S network in budding yeast,
 which also correlates with increased defects in cell growth, cell size,
 and proliferation. This indicates that expansion of the G1/S network in
 budding yeast may represent an evolutionary product of selection for cell
 cycle fitness.
description: Calb_mbp1_C6_0The raw data results of RNA-seq for the sample Calb_mbp1_C6_0Calb_mbp1_C6_30The data results of the RNA-seq for the sample Calb_mbp1_C6_30Delta_swi4_C6_0The data results of the RNA-seq for the sample Delta_swi4_C6_0Delta_swi4_C6_30The data results for RNA-seq for the sample Delta_swi4_C6_30Klac_mbp1_C6_0The RNA-seq data results for the sample Klac_mbp1_C6_0Klac_mbp1_C6_30The RNA-seq data results for the sample Klac_mbp1_C6_30Ncra_BD_C6_0The RNA-seq data results for the sample Ncra_BD_C6_0Ncra_BD_C6_30The RNA-seq data results for the sample Ncra_BD_C6_30RES2_BD_C6_0The RNA-seq data results for the sample RES2_BD_C6_0RES2_BD_C6_30The RNA-seq data results for the sample RES2_BD_C6_30WT_C6_0The RNA seq data results for the sample WT_C6_0WT_C6_30The RNA seq data results for the sample WT_C6_30Calb_mbp1_C7_0The RNA-seq data results for the sample Calb_mbp1_C7_0Calb_mbp1_C7_30The RNA-seq data results for the sample Calb_mbp1_C7_30Delta_swi4_C7_0The RNA-seq data results for the sample Delta_swi4_C7_0Delta_swi4_C7_30The RNA-seq data results for the sample Delta_swi4_C7_30Klac_mbp1_C7_0The RNA-seq data results for the sample Klac_mbp1_C7_0Klac_mbp1_C7_30The RNA-seq data results for the sample Klac_mbp1_C7_30Ncra_BD_C7_0The RNA-seq data results for the sample Ncra_BD_C7_0Ncra_BD_C7_30The RNA-seq data results for the sample Ncra_BD_C7_30RES2_BD_C7_0The RNA-seq data results for the sample RES2_BD_C7_0RES2_BD_C7_30The RNA-seq data results for the sample RES2_BD_C7_30WT_C7_0The RNA-seq data results for the sample WT_C7_0WT_C7_30The RNA-seq data results for the sample WT_C7_30Calb_mbp1_C8_0The RNA-seq data results for the sample Calb_mbp1_C8_0Calb_mbp1_C8_30The RNA-seq data results for the sample Calb_mbp1_C8_30Delta_swi4_C8_0The RNA-seq data results for the sample Delta_swi4_C8_0Delta_swi4_C8_30_The RNA-seq data results for the sample Delta_swi4_C8_30Klac_mbp1_C8_0The RNA-seq data results for the sample Klac_mbp1_C8_0Klac_mbp1_C8_30The RNA-seq data results for the sample Klac_mbp1_C8_30Ncra_BD_C8_0The RNA-seq data results for the sample Ncra_BD_C8_0Ncra_BD_C8_30The RNA-seq data results for the sample Ncra_BD_C8_30RES2_BD_C8_0The RNA-seq data results of the sample RES2_BD_C8_0RES2_BD_C8_30The RNA-seq data results for the sample RES2_BD_C8_30WT_C8_0The RNA-seq data results for the sample WT_C8_0WT_C8_30The RNA-seq data results of the sample WT_C8_30