cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.7924431
title: A fluid-walled microfluidic platform for human neuronal microcircuits and axotomy

description: <strong>ABSTRACT</strong> Recapitulation of human neuronal circuits <em>in vitro</em> will significantly facilitate investigation of human neuronal physiology and pathophysiology. This paper demonstrates and characterises a novel system for culturing neuronal circuits within fluid-walled microenvironments. Fluid-walled technology leverages the immiscibility between aqueous cell media and hydrophobic bio-inert fluorocarbon (FC40) to create miniaturised cell cultures inside standard Petri dishes. Here, we demonstrate the capacity of fluid-walled technology in recreating the human corticostriatal microcircuit. Induced pluripotent stem cell-derived cortical and striatal neurons were co-cultured in fluid-walled dumbbells which, employing intrinsic pressures, allows i) separation of different neuronal subtypes, ii) compartmentalisation of cortical somas and dendrites from cortical axons, and iii) unidirectional projection of cortical axons toward their postsynaptic striatal partner. Exploiting unique features of fluid walls, we also describe a contactless method to perform localised cortical axotomy at pre-defined location along the dumbbells using a micro-jet of cell media. Axotomy of all cortical axonal tracts on the same dish is systematically obtained within 90 seconds. Subsequent axonal regeneration was observed and significantly promoted in the presence of postsynaptic striatal neurons. The ability to control compartmentalisation and directionality of neuronal connections based on fluid dynamics offers an efficient and highly reproducible system for long-term culturing of human neuronal circuits over conventional monoculture of cortical neurons. Combined with reproducible axotomy, we envision this system to be adaptable to large scale screening of regenerative therapeutic candidates in a highly physiological manner. <strong>FILE DESCRIPTIONS</strong> Source Data.xlsx: Tabular datasets plotted on main figures 2 and 5. Key Resources Table.xlsx: Table containing key resources (primary and secondary antibodies, cell lines and software) used in this study.
DOI: 10.5281/zenodo.8164855
title: A fluid-walled microfluidic platform for human neuronal microcircuits and axotomy

description: <strong>ABSTRACT</strong> Recapitulation of human neuronal circuits <em>in vitro</em> will significantly facilitate investigation of human neuronal physiology and pathophysiology. This paper demonstrates and characterises a novel system for culturing neuronal circuits within fluid-walled microenvironments. Fluid-walled technology leverages the immiscibility between aqueous cell media and hydrophobic bio-inert fluorocarbon (FC40) to create miniaturised cell cultures inside standard Petri dishes. Here, we demonstrate the capacity of fluid-walled technology in recreating the human corticostriatal microcircuit. Induced pluripotent stem cell-derived cortical and striatal neurons were co-cultured in fluid-walled dumbbells which, employing intrinsic pressures, allows i) separation of different neuronal subtypes, ii) compartmentalisation of cortical somas and dendrites from cortical axons, and iii) unidirectional projection of cortical axons toward their postsynaptic striatal partner. Exploiting unique features of fluid walls, we also describe a contactless method to perform localised cortical axotomy at pre-defined location along the dumbbells using a micro-jet of cell media. Axotomy of all cortical axonal tracts on the same dish is systematically obtained within 90 seconds. Subsequent axonal regeneration was observed and significantly promoted in the presence of postsynaptic striatal neurons. The ability to control compartmentalisation and directionality of neuronal connections based on fluid dynamics offers an efficient and highly reproducible system for long-term culturing of human neuronal circuits over conventional monoculture of cortical neurons. Combined with reproducible axotomy, we envision this system to be adaptable to large scale screening of regenerative therapeutic candidates in a highly physiological manner. <strong>FILE DESCRIPTIONS</strong> Source Data.xlsx: Tabular datasets plotted on main figures 2 and 5. Key Resources Table.xlsx: Table containing key resources (primary and secondary antibodies, cell lines and software) used in this study.
DOI: 10.5281/zenodo.12684329
title: A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy

description: ABSTRACT



In our brains, different neurons make appropriate connections; however, there remain few in vitro models of such circuits. We use an open microfluidic approach to build and study neuronal circuits in vitro in ways that fit easily into existing bio-medical workflows. Dumbbell-shaped circuits are built in minutes in standard Petri dishes; the aqueous phase is confined by fluid walls – interfaces between cell-growth medium and an immiscible fluorocarbon, FC40. Conditions are established that ensure post-mitotic neurons derived from human induced pluripotent stem cells (iPSCs) plated in one chamber of a dumbbell remain where deposited. After seeding cortical neurons on one side, axons grow through the connecting conduit to ramify amongst striatal neurons on the other – an arrangement mimicking unidirectional cortico-striatal connectivity. We also develop a moderate-throughput non-contact axotomy assay. Cortical axons in conduits are severed by a media jet; then, brain-derived neurotrophic factor and striatal neurons in distal chambers promote axon regeneration. As additional conduits and chambers are easily added, this opens up the possibility of mimicking complex neuronal networks, and screening drugs for their effects on connectivity.

 


FILE DESCRIPTIONS

Source Data.xlsx: Tabular datasets plotted on Main Figures 2, 3 and 6, and Supplementary Figures 1 and 2.

Key Resources Table.xlsx: Table containing details on the key resources (antibodies, cell lines, virus strains, software, code, and protocols) used in this study.
DOI: 10.5281/zenodo.7924430
title: A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy

description: ABSTRACT



In our brains, different neurons make appropriate connections; however, there remain few in vitro models of such circuits. We use an open microfluidic approach to build and study neuronal circuits in vitro in ways that fit easily into existing bio-medical workflows. Dumbbell-shaped circuits are built in minutes in standard Petri dishes; the aqueous phase is confined by fluid walls – interfaces between cell-growth medium and an immiscible fluorocarbon, FC40. Conditions are established that ensure post-mitotic neurons derived from human induced pluripotent stem cells (iPSCs) plated in one chamber of a dumbbell remain where deposited. After seeding cortical neurons on one side, axons grow through the connecting conduit to ramify amongst striatal neurons on the other – an arrangement mimicking unidirectional cortico-striatal connectivity. We also develop a moderate-throughput non-contact axotomy assay. Cortical axons in conduits are severed by a media jet; then, brain-derived neurotrophic factor and striatal neurons in distal chambers promote axon regeneration. As additional conduits and chambers are easily added, this opens up the possibility of mimicking complex neuronal networks, and screening drugs for their effects on connectivity.

 


FILE DESCRIPTIONS

Source Data.xlsx: Tabular datasets plotted on Main Figures 2, 3 and 6, and Supplementary Figures 1 and 2.

Key Resources Table.xlsx: Table containing details on the key resources (antibodies, cell lines, virus strains, software, code, and protocols) used in this study.
DOI: 10.6084/m9.figshare.26198951
title: Idealized_satellite_scenario_mean_values

description: The impact of assimilating the cloud liquid water mixing ratio (<i>q</i><sub><em>c</em></sub>) and other hydrometeor variables on short-term forecasts of downward surface shortwave radiation (DSSR) is evaluated. A perfect-model approach is used to directly verify the nudging scheme of the hydrometeor variables and its subsequent effect. The 4-bit binary data file describes the name of the variable, type of error (either the bias or the RMSE) and the simulation. An instantaneous hourly data starts at 2017/01/01/01:00 and ends at 2017/12/31/24:00, and if "_13oclock" is included in the name, only the data at 13:00 (JST) is used.
DOI: 10.6084/m9.figshare.26198951.v1
title: Idealized_satellite_scenario_mean_values

description: The impact of assimilating the cloud liquid water mixing ratio (<i>q</i><sub><em>c</em></sub>) and other hydrometeor variables on short-term forecasts of downward surface shortwave radiation (DSSR) is evaluated. A perfect-model approach is used to directly verify the nudging scheme of the hydrometeor variables and its subsequent effect. The 4-bit binary data file describes the name of the variable, type of error (either the bias or the RMSE) and the simulation. An instantaneous hourly data starts at 2017/01/01/01:00 and ends at 2017/12/31/24:00, and if "_13oclock" is included in the name, only the data at 13:00 (JST) is used.
DOI: 10.6084/m9.figshare.26198942
title: Idealized_satellite_scenario_RMSEs_5months

description: The impact of assimilating the cloud liquid water mixing ratio (<i>q</i><sub><em>c</em></sub>) and other hydrometeor variables on short-term forecasts of downward surface shortwave radiation (DSSR) is evaluated. A perfect-model approach is used to directly verify the nudging scheme of the hydrometeor variables and its subsequent effect. The 4-bit binary data file describes the name of the variable, type of error (either the bias or the RMSE) and the simulation. An instantaneous hourly data starts at 2017/01/01/01:00 and ends at 2017/12/31/24:00, and if "_13oclock" is included in the name, only the data at 13:00 (JST) is used.
DOI: 10.6084/m9.figshare.26198942.v1
title: Idealized_satellite_scenario_RMSEs_5months

description: The impact of assimilating the cloud liquid water mixing ratio (<i>q</i><sub><em>c</em></sub>) and other hydrometeor variables on short-term forecasts of downward surface shortwave radiation (DSSR) is evaluated. A perfect-model approach is used to directly verify the nudging scheme of the hydrometeor variables and its subsequent effect. The 4-bit binary data file describes the name of the variable, type of error (either the bias or the RMSE) and the simulation. An instantaneous hourly data starts at 2017/01/01/01:00 and ends at 2017/12/31/24:00, and if "_13oclock" is included in the name, only the data at 13:00 (JST) is used.
DOI: 10.15468/dl.ay8z9g
title: Occurrence Download

description: A dataset containing 673 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sillago japonica Temminck & Schlegel, 1843",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 673 records from 32 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016444-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9v8jk5
title: Occurrence Download

description: A dataset containing 468 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Agonum dolens (C.R.Sahlberg, 1827)"
 ]
}

 The dataset includes 468 records from 48 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016445-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.26198915
title: Idealized_satellite_scenario_RMSEs_4months

description: The impact of assimilating the cloud liquid water mixing ratio (<i>q</i><sub><em>c</em></sub>) and other hydrometeor variables on short-term forecasts of downward surface shortwave radiation (DSSR) is evaluated. A perfect-model approach is used to directly verify the nudging scheme of the hydrometeor variables and its subsequent effect. The 4-bit binary data file describes the name of the variable, type of error (either the bias or the RMSE) and the simulation. An instantaneous hourly data starts at 2017/01/01/01:00 and ends at 2017/12/31/24:00, and if "_13oclock" is included in the name, only the data at 13:00 (JST) is used.
DOI: 10.6084/m9.figshare.26198915.v1
title: Idealized_satellite_scenario_RMSEs_4months

description: The impact of assimilating the cloud liquid water mixing ratio (<i>q</i><sub><em>c</em></sub>) and other hydrometeor variables on short-term forecasts of downward surface shortwave radiation (DSSR) is evaluated. A perfect-model approach is used to directly verify the nudging scheme of the hydrometeor variables and its subsequent effect. The 4-bit binary data file describes the name of the variable, type of error (either the bias or the RMSE) and the simulation. An instantaneous hourly data starts at 2017/01/01/01:00 and ends at 2017/12/31/24:00, and if "_13oclock" is included in the name, only the data at 13:00 (JST) is used.
DOI: 10.15468/dl.u6fk78
title: Occurrence Download

description: A dataset containing 441 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pennahia argentata (Houttuyn, 1782)",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 441 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016447-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.umrmgn
title: Occurrence Download

description: A dataset containing 2246 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Agonum ericeti (Panzer, 1809)"
 ]
}

 The dataset includes 2246 records from 81 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016448-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qfmb7d
title: Occurrence Download

description: A dataset containing 733 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bothrocara molle Bean, 1890",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 733 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016449-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.h5k44r
title: Occurrence Download

description: A dataset containing 12 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "GadmGid is CHN.19_1",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Artemisia stolonifera (Maxim.) Kom."
 ]
}

 The dataset includes 12 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016450-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5rp2tk
title: Occurrence Download

description: A dataset containing 185 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Oncorhynchus keta (Walbaum, 1792)",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 185 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016451-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7vxdhn
title: Occurrence Download

description: A dataset containing 339 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Agonum hypocrita (Apfelbeck, 1904)"
 ]
}

 The dataset includes 339 records from 39 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016452-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.35003/ebawg5/rnwtyh
title: fig2.pptx

description: :unav
DOI: 10.35003/ebawg5/ybegze
title: fig1.pptx

description: :unav
DOI: 10.35003/ebawg5/zecbpa
title: fig3.pptx

description: :unav
DOI: 10.35003/ebawg5
title: Replication Data for: The Temperature and Composition of the Mantle Sources of Martian Basalts

description: The composition of basaltic melts in equilibrium with the mantle can be determined for several Martian meteorites and in-situ rover analyses. We use the melting model MAGMARS to reproduce these primary melts and estimate the bulk composition and temperature of the mantle regions from which they originated. We find that most mantle sources are depleted in CaO and Al2O3 relative to models of the bulk silicate Mars and likely represent melting residues or magma ocean cumulates. The concentrations of Na2O, K2O, P2O5, and TiO2 are variable and often less depleted, pointing to the re-fertilization of the sources by fluids and low-degree melts, or the incorporation of residual trapped melts during the crystallization of the magma ocean. The mantle potential temperatures of the sources are 1400–1500°C, regardless of the time at which they melted and within the range of the most recent predictions from thermochemical evolution models.
DOI: 10.35003/ebawg5/anxpq9
title: tables.tab

description: :unav
DOI: 10.15468/dl.r6fvu3
title: Occurrence Download

description: A dataset containing 96 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lagocephalus spadiceus (Richardson, 1845)",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 96 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016453-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yfpvps
title: Occurrence Download

description: A dataset containing 32 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Agonum monachum (Duftschmid, 1812)"
 ]
}

 The dataset includes 32 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016454-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.482r4x
title: Occurrence Download

description: A dataset containing 41 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sebastes cheni Barsukov, 1988",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 41 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016455-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3xm7kp
title: Occurrence Download

description: A dataset containing 11 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Asia",
 "Country is China",
 "GadmGid is CHN.19_1",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Viburnum opulus subsp. calvescens (Rehder) Sugim."
 ]
}

 The dataset includes 11 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016457-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.kfvqky
title: Occurrence Download

description: A dataset containing 33 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "GadmGid is CHN.19_1",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Leontopodium conglobatum (Turcz.) Hand.-Mazz."
 ]
}

 The dataset includes 33 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016458-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2yapr5
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Steno bredanensis (G.Cuvier, 1828)",
 "Geometry POLYGON((170 20, 170 50, 120 50, 120 20, 170 20))",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016459-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vxcan3
title: Occurrence Download

description: A dataset containing 20149433 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is NOR",
 "Year is greater than or equal to 1997",
 "Year is less than or equal to 2018",
 "HasCoordinate is true"
 ]
}

 The dataset includes 20149433 records from 619 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016426-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fm68mt
title: Occurrence Download

description: A dataset containing 762 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Agonum scitulum Dejean, 1828"
 ]
}

 The dataset includes 762 records from 62 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016460-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wygbp4
title: Occurrence Download

description: A dataset containing 17541237 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Portugal",
 "Geometry POLYGON((-9.78323 36.89742,-5.96937 36.89742,-5.96937 42.24968,-9.78323 42.24968,-9.78323 36.89742))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 17541237 records from 961 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016446-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.cvr59m
title: Occurrence Download

description: A dataset containing 80212 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Perca flavescens (Mitchill, 1814)"
 ]
}

 The dataset includes 80212 records from 91 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0337815-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-40560
title: Redemption tidings, vol. 2, nos. 1-12, Jan.-Dec. 1926

description: [description english/roman)] Issued monthly. "A full gospel magazine for all believers" -- subtitle. Bound with vol. 2. Includes Praise and prayer: missionary supplement to Redemption tidings in nos. 2-12 (Feb.-Dec. 1926).