cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.34658/rdb.ff0gm8/xombxp
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/gqxepf
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/0ksrci
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/iq6wxu
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/0yj8ct
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/7sqxne
title: scon2

description: :unav
DOI: 10.34658/rdb.ff0gm8/7hynxj
title: prosol_History

description: :unav
DOI: 10.34658/rdb.ff0gm8/1oavqj
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/zyf9ft
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/quaqjo
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/r8jqrh
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/mtsvqs
title: fq1list

description: :unav
DOI: 10.34658/rdb.ff0gm8/otzvcs
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/puz0l9
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/obaaxq
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/0tvwpn
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/gc8h7h
title: format.temp

description: :unav
DOI: 10.34658/rdb.ff0gm8/i91rtx
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/47y3ju
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/wuegzc
title: format.temp

description: :unav
DOI: 10.34658/rdb.ff0gm8/3zo8r5
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/zyk9d2
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/xnxjoh
title: format.temp

description: :unav
DOI: 10.34658/rdb.ff0gm8/ndof6h
title: prosol_History

description: :unav
DOI: 10.6084/m9.figshare.25479703
title: data

description: <b>Aim</b>: Comparing filtering factors of invasive alien plants (IAP) and non-invasive alien plants (NIAP) provides insight into the successful invasion of alien plants. By examining the distribution of alien plants across prefectures in China, we try to explore the roles of environmental, biotic and dispersal filters in the richness distribution of IAP or NIAP, as well as IAP in four ranks.<b>Location</b>: China.<b>Major taxa studied</b>: naturalized alien plants.<b>Methods</b>: We collected distribution and phylogenetic data for 225 NIAP and 239 IAP across 344 prefectures in China, as well as anthropogenic and environmental data. Furthermore, we categorized IAP into four ranks according to their levels of invasiveness. For each prefecture, we computed standardized effect sizes of mean phylogenetic distance (MPDses) for NIAP, IAP, and four ranks of IAP. Based on general linear mixed-effects models and structural equation models, we examined the roles of biotic, environmental, and dispersal filters on the richness of NIAP and IAP, including those in four invasiveness ranks.<b>Results</b>: The phylogenetic structure of IAP was more clustered than that of NIAP. We found that VT and GDP were more important for NIAP than for IAP, while MPDses had a strong negative effect on IAP than on NIAP. MPD was positively related to IAP but negatively related to NIAP. Furthermore, we observed more clustered phylogenetic structures in rank I, which includes the IAP with the most serious impacts. Dispersal filters were found to be particularly critical for rank I, while biotic filters played a more important role in the slightly impact IAP (rank IV).<b>Main conclusions</b>: Successful invasion necessitates a heightened ability for spreading and competition. Interspecies competition between IAP and NIAP restricts the distribution of NIAP. Therefore, considering resident alien species will help us predict which groups are more susceptible to invasion.
DOI: 10.6084/m9.figshare.25479703.v1
title: data

description: <b>Aim</b>: Comparing filtering factors of invasive alien plants (IAP) and non-invasive alien plants (NIAP) provides insight into the successful invasion of alien plants. By examining the distribution of alien plants across prefectures in China, we try to explore the roles of environmental, biotic and dispersal filters in the richness distribution of IAP or NIAP, as well as IAP in four ranks.<b>Location</b>: China.<b>Major taxa studied</b>: naturalized alien plants.<b>Methods</b>: We collected distribution and phylogenetic data for 225 NIAP and 239 IAP across 344 prefectures in China, as well as anthropogenic and environmental data. Furthermore, we categorized IAP into four ranks according to their levels of invasiveness. For each prefecture, we computed standardized effect sizes of mean phylogenetic distance (MPDses) for NIAP, IAP, and four ranks of IAP. Based on general linear mixed-effects models and structural equation models, we examined the roles of biotic, environmental, and dispersal filters on the richness of NIAP and IAP, including those in four invasiveness ranks.<b>Results</b>: The phylogenetic structure of IAP was more clustered than that of NIAP. We found that VT and GDP were more important for NIAP than for IAP, while MPDses had a strong negative effect on IAP than on NIAP. MPD was positively related to IAP but negatively related to NIAP. Furthermore, we observed more clustered phylogenetic structures in rank I, which includes the IAP with the most serious impacts. Dispersal filters were found to be particularly critical for rank I, while biotic filters played a more important role in the slightly impact IAP (rank IV).<b>Main conclusions</b>: Successful invasion necessitates a heightened ability for spreading and competition. Interspecies competition between IAP and NIAP restricts the distribution of NIAP. Therefore, considering resident alien species will help us predict which groups are more susceptible to invasion.
DOI: 10.34658/rdb.ff0gm8/020wyb
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/ullbwp
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/oq5cgp
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/cw1qem
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/eix6cn
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/c8y8za
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/s9r1rg
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/943y4y
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/sz2eqp
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/ik76iv
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/idpoyd
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/5uodtb
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/r38prw
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/b6ghf3
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/fgy54q
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/pjwakp
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/q40omu
title: scon2

description: :unav
DOI: 10.34658/rdb.ff0gm8/zonpwb
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/gusbqh
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/2vh2rd
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/eeitmw
title: fid

description: :unav
DOI: 10.34658/rdb.ff0gm8/nytk7o
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/rno9u2
title: auditc.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/k0iydy
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/p65gdw
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/bqf2uh
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/gy6baq
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/ugjnhn
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/i5eqdr
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/ygvdil
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/hotifj
title: prosol_History

description: :unav
DOI: 10.34658/rdb.ff0gm8/pjxv10
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/gsszda
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/9rcvl5
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/zs0mtd
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/41rssg
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/fuewxh
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/qhaczh
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/tscbvi
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/sysnlv
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/p6sf0n
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/gyzpdp
title: format.temp

description: :unav
DOI: 10.34658/rdb.ff0gm8/dbgfdz
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/owuzbl
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/logztb
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/4zj0ae
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/a62cz7
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/fkyulh
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/ihxwyg
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/p7obup
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/rmadom
title: fid

description: :unav
DOI: 10.34658/rdb.ff0gm8/jmycew
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/mpegxi
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/gyvi1j
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/dvb3ls
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/7z3vsv
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/wzfvte
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/srv6nf
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/2beria
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/t76ajv
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/r61oow
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/obvco4
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/80z7ag
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/ppib8g
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/yngrsp
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/w4kzcc
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/kxdhgu
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/66lknc
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/q2pjoh
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/6bnxmu
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/etuaze
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/eqa3ub
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/itysqx
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/dflanp
title: format.temp

description: :unav
DOI: 10.34658/rdb.ff0gm8/eve1fz
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/mtkhe5
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/w3kxvq
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/djsh2l
title: fq1list

description: :unav
DOI: 10.34658/rdb.ff0gm8/ijbmci
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/gaaod0
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/kmkxvk
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/wyr4md
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/dc7xlk
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/xln5rq
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/bg2ug2
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/j4vbjb
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/ybbd1h
title: fid

description: :unav
DOI: 10.34658/rdb.ff0gm8/ljhnda
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/vmbodn
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/xe2low
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/so1je8
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/oo59xr
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/af09wk
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/l7zzeu
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/0hzi9d
title: auditc.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/jgb43h
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/ohdezl
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/l0s805
title: fid

description: :unav
DOI: 10.34658/rdb.ff0gm8/fao4db
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/n79ef9
title: fq1list

description: :unav
DOI: 10.34658/rdb.ff0gm8/04rdwi
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/s5mdib
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/4f4hzt
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/2catgh
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/fvwoq3
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/xg4izg
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/nh9rsr
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/qtcmwm
title: acqu

description: :unav
DOI: 10.34658/rdb.ff0gm8/nkovyh
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/hapgzz
title: audita.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/duuy8s
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/h7knx4
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/01wb04
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/2cigv3
title: procs

description: :unav
DOI: 10.34658/rdb.ff0gm8/sx3mpv
title: format.ased

description: :unav
DOI: 10.34658/rdb.ff0gm8/3hcn3i
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/pkrbi7
title: fid

description: :unav
DOI: 10.34658/rdb.ff0gm8/cl45ch
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/v5ka8m
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/mz1eyl
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/c85d0c
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/ywfsmq
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/1xq4ao
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/pz2ujo
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/hmxp3w
title: pulseprogram

description: :unav
DOI: 10.34658/rdb.ff0gm8/5b3p4i
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/se02pk
title: fid

description: :unav
DOI: 10.34658/rdb.ff0gm8/vevgkb
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/dh4czr
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/wsgqel
title: thumb.png

description: :unav
DOI: 10.34658/rdb.ff0gm8/6ucqvn
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/peehag
title: 1r

description: :unav
DOI: 10.34658/rdb.ff0gm8/mpy2oe
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/edyomh
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/8kpwov
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/s0xtwv
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/pif8zj
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/riys52
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/qe3apk
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/hf1dfo
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/3uuczx
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/wpliqr
title: acqus

description: :unav
DOI: 10.34658/rdb.ff0gm8/quy8or
title: format.temp

description: :unav
DOI: 10.34658/rdb.ff0gm8/bou6vt
title: specpar

description: :unav
DOI: 10.34658/rdb.ff0gm8/j3list
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/fqhe3u
title: scon2

description: :unav
DOI: 10.34658/rdb.ff0gm8/mwovyb
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/izxre8
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/btxhyn
title: cpdprg2

description: :unav
DOI: 10.34658/rdb.ff0gm8/f5ci2i
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/6wdr8p
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/bugqtb
title: title

description: :unav
DOI: 10.6084/m9.figshare.20472768.v1
title: Biodiversity estimates of associated faunal assemblages of free-living <i>Fucus vesiculosus</i>

description: Macroinfaunal and epifaunal assemblages associated with Baltic Sea free-living <i>Fucus vesiculosus</i> by conventional sampling (benthic cores and quadrats) and eDNA (V4 region of the 18S nuclear small subunit (nSSU) ribosomal RNA).
DOI: 10.34658/rdb.ff0gm8/kze6dc
title: specpar

description: :unav
DOI: 10.6084/m9.figshare.20472768
title: Biodiversity estimates of associated faunal assemblages of free-living <i>Fucus vesiculosus</i>

description: Macroinfaunal and epifaunal assemblages associated with Baltic Sea free-living <i>Fucus vesiculosus</i> by conventional sampling (benthic cores and quadrats) and eDNA (V4 region of the 18S nuclear small subunit (nSSU) ribosomal RNA).
DOI: 10.34658/rdb.ff0gm8/jfv0cc
title: outd

description: :unav
DOI: 10.34658/rdb.ff0gm8/lbicjd
title: 1i

description: :unav
DOI: 10.34658/rdb.ff0gm8/dg8as8
title: auditp.txt

description: :unav
DOI: 10.34658/rdb.ff0gm8/cfwcus
title: shimvalues

description: :unav
DOI: 10.34658/rdb.ff0gm8/352ga5
title: uxnmr.par

description: :unav
DOI: 10.34658/rdb.ff0gm8/lfmgyx
title: proc

description: :unav
DOI: 10.34658/rdb.ff0gm8/ik2gby
title: uxnmr.info

description: :unav
DOI: 10.34658/rdb.ff0gm8/nq69vd
title: scon2

description: :unav
DOI: 10.34658/rdb.ff0gm8/vtt4tp
title: title

description: :unav
DOI: 10.34658/rdb.ff0gm8/xgbn1a
title: scon2

description: :unav
DOI: 10.5281/zenodo.10875911
title: Size-Dependent Dynamics of the Internal Carbon Pool Drive Isotopic Vital Effects in Calcifying Phytoplankton

description: Isotopic offsets in biogenic calcite from equilibrium values can provide unique insights into the physiology and mechanisms of carbon regulation in calcifying phytoplankton. This study examines the impact of varying CO2 (controlled via pH) on five coccolithophore species chosen for varied cell sizes, physiology, and calcification. The study investigates isotopic offsets in coccolith calcite and organic matter, in relation to carbon demand and supply (µ/CO2). Species-specific CO2/pH optima for growth (µopt) were derived from variations in growth rates with varying CO2 concentrations. Growth rates for all species declined with rising CO2 (decreasing pH) due to H+-driven inhibition. C. braarudii and C. leptoporus exhibited high µopt values (suggesting high carbon-demand) and limited growth under low CO2 (high pH) suggesting carbon limitation. Under low CO2 supply, when growth rates were CO2-limited, both species exhibited coincident isotopic depletion in calcite and organic matter as a consequence of CO2 diffusion into the cell that experienced no equilibration as a result of a highly depleted internal carbon pool. In these two species, isotopic values in calcite  remained unaffected by growth rates and CO2 concentration (µ/CO2) when CO2 was sufficient for optimal growth. G. huxleyi and G. oceanica displayed optima for growth (µopt) at low CO2 concentrations and showed no growth limitation under low CO2 (indicating low carbon-demand). Both species experienced depleted (negative) vital effects caused by an excess of CO2 diffusion into the small internal carbon pool of the cell when diffusive carbon supply outpaced low demand (low µ/CO2). Enriched (positive) vital effects were observed under low carbon supply and high demand, likely due to diffusive loss of CO2 from the cell's intracellular carbon pool due to a lower intracellular pH. C. carterae exhibited an intermediate µopt and isotopically equilibrated intracellular carbon pool such that δ13C values in calcite and organic matter suggested a shared carbon pool. This study illustrates that pH-CO2 driven vital effects and fractionation into organic matter indicate the residence time for carbon in the intracellular carbon pool, and the size of this pool is proportional to cell size. C. leptoporus and C. carterae can elevate intracellular pH to minimise CO2 leakage, whereas vital effects in G. huxleyi and G. oceanica are caused by diffusive CO2 due to the small size of their internal carbon pool.
DOI: 10.5281/zenodo.8265649
title: Size-Dependent Dynamics of the Internal Carbon Pool Drive Isotopic Vital Effects in Calcifying Phytoplankton

description: Isotopic offsets in biogenic calcite from equilibrium values can provide unique insights into the physiology and mechanisms of carbon regulation in calcifying phytoplankton. This study examines the impact of varying CO2 (controlled via pH) on five coccolithophore species chosen for varied cell sizes, physiology, and calcification. The study investigates isotopic offsets in coccolith calcite and organic matter, in relation to carbon demand and supply (µ/CO2). Species-specific CO2/pH optima for growth (µopt) were derived from variations in growth rates with varying CO2 concentrations. Growth rates for all species declined with rising CO2 (decreasing pH) due to H+-driven inhibition. C. braarudii and C. leptoporus exhibited high µopt values (suggesting high carbon-demand) and limited growth under low CO2 (high pH) suggesting carbon limitation. Under low CO2 supply, when growth rates were CO2-limited, both species exhibited coincident isotopic depletion in calcite and organic matter as a consequence of CO2 diffusion into the cell that experienced no equilibration as a result of a highly depleted internal carbon pool. In these two species, isotopic values in calcite  remained unaffected by growth rates and CO2 concentration (µ/CO2) when CO2 was sufficient for optimal growth. G. huxleyi and G. oceanica displayed optima for growth (µopt) at low CO2 concentrations and showed no growth limitation under low CO2 (indicating low carbon-demand). Both species experienced depleted (negative) vital effects caused by an excess of CO2 diffusion into the small internal carbon pool of the cell when diffusive carbon supply outpaced low demand (low µ/CO2). Enriched (positive) vital effects were observed under low carbon supply and high demand, likely due to diffusive loss of CO2 from the cell's intracellular carbon pool due to a lower intracellular pH. C. carterae exhibited an intermediate µopt and isotopically equilibrated intracellular carbon pool such that δ13C values in calcite and organic matter suggested a shared carbon pool. This study illustrates that pH-CO2 driven vital effects and fractionation into organic matter indicate the residence time for carbon in the intracellular carbon pool, and the size of this pool is proportional to cell size. C. leptoporus and C. carterae can elevate intracellular pH to minimise CO2 leakage, whereas vital effects in G. huxleyi and G. oceanica are caused by diffusive CO2 due to the small size of their internal carbon pool.