DOI: 10.5281/zenodo.14838020
title: Behaviour and Reproduction of Drosophila Melanogaster Exposed to 3.5 GHz Radio-Frequency Electromagnetic Fields - Underlying data

description: Insects are exposed to radio-frequency electromagnetic fields emitted by wireless telecommunication networks. A part of these fields will be absorbed inside of these insects. This absorption might have biological effects, depending on the amount of absorbed power. It is currently unknow at what level of absorption this might occur. To investigate this, we used RF dosimetry of adult D. melanogaster flies, which we combined with two assays studying the locomotor activity and fecundity of D. melanogaster exposed to electromagnetic fields at 3.6 GHz. To perform dosimetry, we created a 3D digital model of an adult fly using micro-CT scans of a female D. melanogaster. We used this model in numerical EM simulations to estimate the absorbed power in the fly as a function of RF frequency in the far field of an antenna and during the two experimental assays at 3.6 GHz. No effects were found on locomotor activity for a five-day exposure to 3.6 GHz between 5.4 and 9 V/m, which correspond to 3.56 nW to 9.88 nW of absorbed power. We also did not find any effects on fecundity, at an absorption level of 1.91 mW during 48h at 3.6 GHz during 48h. In our future work, we aim to investigate this exposure at higher frequencies and for immature stages as well.
description: Overview

This dataset contains segmented STL 3D models, TIFF imaging data, and visualisations of the Drosophila (fruit fly) scan used in this study 

File Index

STL 3D Models (Drosophila.zip)

These files represent different anatomical structures of Drosophila in STL format for 3D visualization, printing, and analysis.






File Name
Description
File Size




Drosophila_brain.stl
3D model of the fruit fly brain
9.79 MB


Drosophila_exoskeleton.stl
External structure/exoskeleton
42.6 MB


Drosophila_gonads.stl
Reproductive organs
42.9 KB


Drosophila_halteres.stl
Small hind-wing balancing organs
2.55 MB


Drosophila_internal_tissue.stl
Internal soft tissues
10.6 MB


Drosophila_muscles.stl
Muscle structures
1.71 MB


Drosophila_wing_left.stl
Left wing structure
2.07 MB


Drosophila_wing_right.stl
Right wing structure
2.11 MB





Metadata

Name: Drosophila melanogaster- Blue Bottle Fly - AdultOrder: DipteraFamily: DrosophilidaeSpecies: Drosophila melanogasterStage: adult - femaleOrigin: Reared in the lab of Herman Wijnen at University of Southampton, UK, in 2022Classification: 2022 by Herman Wijnen of University of Southampton, UK, in 2022Preservation: Following 5 min incubation in 0.5% Triton X-100 PBS, the flies were fixed in Bouin’s solution for 24h, washed in 1x PBS and then stained for 2d in Lugol’s solution prior to washing in 1x PBS and scanning in ultrapure water.Scanning:  Done by dr. Orestis Katsamenis using a custom Nikon XTH225ST μCT system. The X-ray tube was operated at 80 kVp and 36 μA, delivering a power of 2.88 W.

TIFF Imaging Data

These TIFF files contain μCT -based X-ray histology (XRH) scans of the Drosophila specimen.






File Name
Description
File Size




20221110_XRH_3175_OLK_MBIO07123-FIXED__0001.tif
Individual XRH image slice
4.1 MB


20221110_XRH_3175_OLK_MBIO07123-FIXED__0250.tif
Individual XRH image slice
4.1 MB


23330_20221110_XRH_3175_OLK_MBIO07123-FIXED_692x960x1152x16bit.tif
Full Drosophila volume dataset
1.5 GB





 

The .png files are artistic renderings of average and standard deviation projections, mapped as different color channels.






File Name
Description
File Size




Composite_1.png
Composite projection rendering
897.9 kB


Composite_2.png
Composite projection rendering
897.9 kB


Composite_3.png
Composite projection rendering
1.1 MB


Composite_4.png
Composite projection rendering
1.0 MB


Montage-5x2.png
Montage of 10 axial slices across Drosophila
182.5 kB





Compressed Archives

These .zip files contain multiple dataset components for easier distribution.






File Name
Description
File Size




Drosophila.zip
Archive containing all STL files listed above
39.3 MB


tiffStackx16bit.zip
Archive containing TIFF imaging data (stacked slices)
854.4 MB





Usage



STL models can be opened in MeshLab, Blender, 3D Slicer, Paraview, etc

TIFF imaging data can be analysed using ImageJ, Fiji, Avizo, or 3D Slicer, etc

Montage-5x2.png provides an overview of axial slices across the specimen.


Attribution

If using these files in research, please cite the appropriate sources.