cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.fxxucd
title: Occurrence Download

description: A dataset containing 10 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Distichium capillaceum var. capillaceum",
 {
 "or" : [
 "Geometry POLYGON((-23.203125 67.339860,-23.203125 40.979898,47.8125 40.979898,47.8125 67.339860,-23.203125 67.339860))",
 "Geometry POLYGON((8.4375 80.415707,8.4375 42.553080,52.03125 42.553080,52.03125 80.415707,8.4375 80.415707))"
 ]
 }
 ]
}

 The dataset includes 10 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029668-160910150852091/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/examiner-c44-84943
title: Rams versus Cleveland Browns, 1955

description: 31 images. Rams versus Cleveland Browns. December 26 1955. "Sports"; Negatives only; (Sleeve reads: SP-11780.)
DOI: 10.15468/dl.xwpw7u
title: Occurrence Download

description: A dataset containing 17932522 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "IucnRedListCategory is one of (VU, EN, NT, LC)",
 "OccurrenceStatus is Present",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 17932522 records from 1014 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003861-231120084113126/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.hry84q
title: Occurrence Download

description: A dataset listing the 1 species recorded in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "TaxonKey is Michelia figo (Lour.) Spreng."
 ]
}

 The dataset's 1 records were derived from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0063751-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-42315
title: Redemption tidings, vol. 10, nos. 1-24, 1 Jan. - 15 Dec. 1934

description: [description english/roman)] Issued fortnightly (every two weeks). "A full gospel magazine for all believers" -- subtitle. Includes Missionary supplement to Redemption tidings (1 Jan. - 15 Apr. 1934) and Tidings from our missionaries (1 May - 15 Dec. 1934) in each issue.
DOI: 10.15468/dl.qqbecj
title: Occurrence Download

description: A dataset containing 3556 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Seseli annuum L., Seseli glabratum Willd. ex Schult.)",
 "Geometry POLYGON((-9.36035 44.71551,-10.59082 42.81152,-11.82129 36.80928,-7.69043 36.17336,-3.38379 35.88905,3.12012 38.13456,9.18457 38.20366,11.7334 37.16032,12.78809 34.74161,17.09473 33.79741, 23.15918 34.23451,30.10254 32.62087,33.2666 32.99024,35.72754 35.03,33.00293 35.53223,28.69629 35.17381,28.34473 36.31513,25.7959 37.50973,24.91699 39.90974,28.78418 40.6473,30.71777 41.17865,36.87012 45.213,38.18848 46.98025,38.71582 47.7541,39.94629 47.93107,40.20996 49.61071,37.0459 50.34546,35.72754 50.40152,33.79395 52.26816,31.68457 51.94426,31.4209 53.27835,32.56348 53.43572,30.98145 54.77535,30.89355 55.52863,28.43262 56.02295,27.20215 57.65716,27.37793 58.30949,27.90527 59.17593,29.66309 59.88894,27.6416 60.58697,31.50879 62.75473,28.78418 69.83962,30.89355 70.28912,29.22363 71.55274,21.75293 71.30079,14.28223 70.14036,11.38184 67.47492,4.87793 63.66576,-5.53711 71.52491,-10.63477 71.69129,-21.5332 67.74276,-28.56445 65.36684,-24.3457 61.35461,-9.36035 44.71551))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2021"
 ]
}

 The dataset includes 3556 records from 87 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0252988-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-42307
title: Redemption tidings, vol. 10, nos. 1-24, 1 Jan. - 15 Dec. 1934

description: [description english/roman)] Issued fortnightly (every two weeks). "A full gospel magazine for all believers" -- subtitle. Includes Missionary supplement to Redemption tidings (1 Jan. - 15 Apr. 1934) and Tidings from our missionaries (1 May - 15 Dec. 1934) in each issue.
DOI: 10.15468/dl.k9mbd2
title: Occurrence Download

description: A dataset containing 11 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "TaxonKey is Osmunda regalis L."
 ]
 },
 "HasCoordinate is TRUE",
 "Year is greater than or equal to 2010",
 "Year is less than or equal to 2020",
 "DecimalLatitude is greater than or equal to 45.2",
 "DecimalLatitude is less than or equal to 62.3",
 "DecimalLongitude is greater than or equal to -77.3",
 "DecimalLongitude is less than or equal to -60.3"
 ]
}

 The dataset includes 11 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0194222-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.utthym
title: Occurrence Download

description: A dataset containing 28 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "TaxonKey is Ursus arctos Linnaeus, 1758"
 ]
}

 The dataset includes 28 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0063762-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fz5vq4
title: Occurrence Download

description: A dataset containing 13288 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((-76.6462501999999972 39.3441758000000021, -76.6462501999999972 39.3484166499999972, -76.6462501999999972 39.3517739899999981, -76.6462501999999972 39.3542478099999968, -76.6462501999999972 39.3576051499999977, -76.6462501999999972 39.3618459999999999, -76.6434266499999950 39.3618459999999999, -76.6391916999999978 39.3618459999999999, -76.6335451000000063 39.3618459999999999, -76.6303688899999997 39.3618459999999999, -76.6263103900000004 39.3618459999999999, -76.6236633000000040 39.3618459999999999, -76.6236633000000040 39.3586653600000034, -76.6236633000000040 39.3565449399999991, -76.6236633000000040 39.3533643000000026, -76.6236633000000040 39.3501836699999998, -76.6236633000000040 39.3466496300000017, -76.6236633000000040 39.3441758000000021, -76.6271926699999995 39.3441758000000021, -76.6296630599999986 39.3441758000000021, -76.6337215599999979 39.3441758000000021, -76.6388387899999941 39.3441758000000021, -76.6414856300000054 39.3441758000000021, -76.6462501999999972 39.3441758000000021))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1932",
 "BasisOfRecord is one of (Human Observation, Living Specimen, Observation, Specimen, Machine Observation)"
 ]
}

 The dataset includes 13288 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0101540-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-42351
title: Redemption tidings, vol. 10, nos. 1-24, 1 Jan. - 15 Dec. 1934

description: [description english/roman)] Issued fortnightly (every two weeks). "A full gospel magazine for all believers" -- subtitle. Includes Missionary supplement to Redemption tidings (1 Jan. - 15 Apr. 1934) and Tidings from our missionaries (1 May - 15 Dec. 1934) in each issue.
DOI: 10.15468/dl.3xku4r
title: Occurrence Download

description: A dataset containing 52551 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rana arvalis Nilsson, 1842"
 ]
}

 The dataset includes 52551 records from 155 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0157868-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.r2fmjq
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Cyperus pseudopeteri (Goetgh.) Bauters"
 ]
}

 The dataset includes 3 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0061782-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.u8cvem
title: Occurrence Download

description: A dataset containing 22 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Vermivora pinus (Linnaeus, 1766)",
 "HasCoordinate is True",
 "Country is United States of America",
 "Year is greater than or equal to 1999",
 "Year is less than or equal to 2001",
 "Month is greater than or equal to January",
 "Month is less than or equal to December",
 "HasGeospatialIssue is False",
 "DecimalLatitude is greater than or equal to 24.5",
 "DecimalLatitude is less than or equal to 49.5",
 "DecimalLongitude is greater than or equal to -125.0",
 "DecimalLongitude is less than or equal to -66.5"
 ]
}

 The dataset includes 22 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0360773-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-42337
title: Redemption tidings, vol. 10, nos. 1-24, 1 Jan. - 15 Dec. 1934

description: [description english/roman)] Issued fortnightly (every two weeks). "A full gospel magazine for all believers" -- subtitle. Includes Missionary supplement to Redemption tidings (1 Jan. - 15 Apr. 1934) and Tidings from our missionaries (1 May - 15 Dec. 1934) in each issue.
DOI: 10.15468/dl.q6axhv
title: Occurrence Download

description: A dataset containing 7 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Macaca tonkeana (Meyer, 1899))",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Machine Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 7 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0061760-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.xp2kz8
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is one of (Hyperthermus butylicus Zillig et al., 1991, Geogemma pacifica Kashefi, Holmes & Lovley, 2006, Geogemma indica Kashefi, Holmes & Lovley, 2006, Acantholithium stellatum Haeckel, 1887, Acantholithium dicopum Haeckel, 1887)"
 ]
}

 The dataset includes 3 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0194216-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5f6kz7
title: Occurrence Download

description: A dataset containing 235 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "Continent is South America",
 "Continent is North America",
 "Continent is Africa"
 ]
 },
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Caesalpinia pulcherrima (L.) Sw.",
 "Year 2000-2022"
 ]
}

 The dataset includes 235 records from 45 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0363446-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.m3354h
title: Occurrence Download

description: A dataset containing 47 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Cyperus metallorum (P.A.Duvign. & G.Léonard) Bauters"
 ]
}

 The dataset includes 47 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0061755-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4squry
title: Occurrence Download

description: A dataset containing 2461 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is one of (Ogdeniella pusilla (Chardez, 1977) Golemansky, 1982, Thermococcus hydrothermalis Godfroy et al., 1997, Amphorellopsis pusilla Chardez, 1977, Ogdeniella maxima (Golemansky, 1970) Golemansky, 1982, Thermococcus pacificus Miroshnichenko et al., 1998, Acanthoplegma krohni, Amphorellopsis maximus Golemansky, 1970, Thermococcus gorgonarius Miroshnichenko et al., 1998, Ogdeniella lucida (Golemansky, 1970) Golemansky, 1982, Thermococcus gammatolerans Jolivet et al., 2003, Micramphora hellebauti Chardez, 1971, Thermococcus fumicolans Godfroy & Meunier, 1996, Acanthochiasma fusiforme Haeckel, 1861, Amphorellopsis lucida Golemansky, 1970, Thermococcus coalescens Kuwabara et al., 2005, Ogdeniella elegans (Golemansky, 1970) Golemansky, 1982, Thermococcus chitonophagus Huber & Stetter, 1996, Amphorellopsis elegans Golemansky, 1970, Thermococcus celericrescens Kuwabara et al., 2007, Ogdeniella carinata (Chardez, 1977) Golemansky, 1982, Amphorellopsis carinata Chardez, 1977, Thermococcus barossii Duffaud et al., 2005, Tuscaridium cygneum Murray, 1885, Thermococcus barophilus Marteinsson et al., 1999, Thermococcus atlanticus Cambon-Bonavita et al., 2004, Micropsammella retorta Golemansky, 1970, Thermococcus alcaliphilus Keller et al., 1997, Peridium spinipes Haeckel, 1887, Micropsammella minima Chardez & Thomas, 1980, Thermococcus guaymasensis Canganella et al., 1998, Peridium longispinum Joergensen, 1900, Micropsammella infralittoralis Chardez, 1993, Thermococcus aggregans Canganella et al., 1998, Thermococcus aegaeus Arab et al., 2000, Micramphora tokioensis Sudzuki, 1979, Micramphora pontica Valkanov, 1970, Thermococcus acidaminovorans Dirmeier et al., 2001, Micramphora atlantica Chardez & Thomas, 1980, Micramphora amphoriformis Chardez & Thomas, 1980, Peridium minutum Popofsky, 1908, Chardezia caudata Golemansky, 1970, Alepiella tricornuta Golemansky, 1970, Lesquerella mesopsammophila Chardez & Thomas, 1980, Collozoum inerme (J.Müller, 1856), Sphaerozoum inerme J.Müller, 1856, Gromia solenopus Zarnik, 1907, Methanohalobium evestigatum Zhilina & Zavarzin, 1988, Gromia liasica Terquem, 1866, Gromia granulata Schulze, 1875, Gromia fluvialis Dujardin, 1837)"
 ]
}

 The dataset includes 2461 records from 77 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0194233-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-42157
title: Redemption tidings, vol. 9, nos. 1-12, Jan.-Dec. 1933

description: [description english/roman)] Issued monthly. "A full gospel magazine for all believers" -- subtitle. Bound with vol. 7-8. Includes Missionary supplement to Redemption tidings in each issue (Jan.-Dec. 1933).
DOI: 10.15468/dl.4fdkyj
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Oedipina tzutujilorum Brodie, Acevedo & Campbell, 2012)",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Machine Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0061799-230224095556074/datasets/export for details.
DOI: 10.15468/dl.gvd3f4
title: Occurrence Download

description: A dataset containing 35676 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Anredera cordifolia (Ten.) Steenis, Anthemis tomentosa L., Anthemis triumfetti (L.) DC., Anthriscus caucalis M.Bieb.)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 35676 records from 452 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0143535-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-40789
title: Redemption tidings, vol. 3, nos. 1-12, Jan.-Dec. 1927

description: [description english/roman)] Issued monthly. "A full gospel magazine for all believers" -- subtitle. Bound with vol. 4. Includes Praise and prayer: missionary supplement to Redemption tidings in each issue (Jan.-Dec. 1927).
DOI: 10.15468/dl.dgxqux
title: Occurrence Download

description: A dataset containing 72949 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Alytes obstetricans (Laurenti, 1768), Alytes obstetricians Laurenti)",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Machine Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 72949 records from 714 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0061816-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.23zgsk
title: Occurrence Download

description: A dataset containing 146313 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Anastomus Bonnaterre, 1791",
 "Year 2000-2022"
 ]
}

 The dataset includes 146313 records from 44 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0157847-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15228103
title: TEMPEST_LIC_cycling_data_1

description: Cycling data for LIC cells on project TEMPEST. Data included is single capacity test cycles at C/2, C/5, and C/10, at temperature conditions 5 °C, 15 °C, 25 °C, and 35 °C.
DOI: 10.5281/zenodo.15228104
title: TEMPEST_LIC_cycling_data_1

description: Cycling data for LIC cells on project TEMPEST. Data included is single capacity test cycles at C/2, C/5, and C/10, at temperature conditions 5 °C, 15 °C, 25 °C, and 35 °C.
DOI: 10.15468/dl.qakcbc
title: Occurrence Download

description: A dataset containing 474 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Blennius ocellaris Linnaeus, 1758",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Specimen, Material sample)"
 ]
 }
 },
 {
 "or" : [
 "CoordinateUncertaintyInMeters is less than 10000",
 "CoordinateUncertaintyInMeters is null"
 ]
 }
 ]
}

 The dataset includes 474 records from 31 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002147-250415084134356/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nffxru
title: Occurrence Download

description: A dataset containing 8196 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Formicidae Collection Univesity of Granada"
 ]
}

 The dataset includes 8196 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002146-250415084134356/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.17918071
title: BBE Reproducibility Package (No Source Code): Coherence–Diversity Invariant and Organisational Equilibrium
title: organisational equilibrium, coherence–diversity invariant, complex systems, reproducibility dataset, Noether-consistent flow, Berchie Behaviour Ensemble, GB2521475.0, conservation law, response function, frequency cutoff

description: This repository contains reproducibility data, pseudocode outlines, figures, and parameter grids associated with the Berchie Behaviour Ensemble (BBE) framework, developed by Derek Osei Berchie (UK patent application GB2521475.0).

The BBE framework defines an organisational invariant derived from coherence (B^{(CI)}) and diversity (B^{(DI)}) indices computed from ensemble spectra, and identifies a dynamically stable equilibrium between these quantities.

This package provides all outputs required for independent verification of the reported results, including convergence to equilibrium, shock–recovery stability, and frequency-dependent organisational response (cutoff frequency f_c ≈ 0.0275).

Source code is intentionally omitted; only pseudocode and data files are provided to reproduce the reported measurements.

All contents are copyright © Derek Osei Berchie 2025. All rights reserved. Verification use only.

Related patent: GB2521475.0 – “Systems and Methods for Monitoring, Diagnosing, Predicting and Controlling Technical Systems Using Organisational State Metrics Derived from Spectral Coherence and Diversity.”
DOI: 10.15468/dl.8az48c
title: Occurrence Download

description: A dataset containing 94 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((119.52243 29.77082,120.86127 29.77082,120.86127 30.87146,119.52243 30.87146,119.52243 29.77082))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Hesperiidae"
 ]
}

 The dataset includes 94 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0051752-251120083545085/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.u5fvbs
title: Occurrence Download

description: A dataset containing 3182 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Furcraea foetida",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 {
 "or" : [
 "CoordinateUncertaintyInMeters is less than 10000",
 "CoordinateUncertaintyInMeters is null"
 ]
 }
 ]
}

 The dataset includes 3182 records from 139 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0051753-251120083545085/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.17926122
title: Genetically Targeted Antifungal Therapeutics: A CRISPR-dCas9--RNAi Hybrid Framework with Dual-Gene Targeting, Evolutionary Escape Modeling, and In Vitro Validation Protocol

description: This study introduces an advanced framework for Genetically Targeted Antifungal Therapeutics (GTAT), incorporating dual-gene silencing of \textit{ERG11} and \textit{FKS1}, probabilistic modeling of evolutionary escape, and a comprehensive \textit{in vitro} validation protocol. Although the core model relies on synthetic mRNA data for demonstration, this limitation is explicitly acknowledged, with recommendations for RT-qPCR quantification in \textit{Candida albicans} and cytotoxicity assessments in HepG2 cells to confirm specificity and safety. The framework further models nuclease degradation kinetics, macrophage clearance, and potential immune activation by chitosan-lipid nanoparticles (CLNPs). Dual-gene knockdown reduces the evolutionary escape probability from $2.4 \times 10^{-3}$ (single-target) to $5.8 \times 10^{-6}$ (dual-target). All assertions are designed to be falsifiable, reproducible, and firmly rooted in established genomic, pharmacological, and immunological principles.