cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.13145/bacdive114303.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive114304.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115146.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115147.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115148.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115149.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115150.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115151.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115152.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115153.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115154.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115996.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive115997.20190402.4
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.6084/m9.figshare.24251011
title: SRR3414635 (GSE80550) bam and index files

description: ////
DOI: 10.6084/m9.figshare.24251011.v1
title: SRR3414635 (GSE80550) bam and index files

description: ////
DOI: 10.21223/zhdp8m/hgdcmz
title: TVIT200412_AYMARA_Cooking.xls

description: :unav
DOI: 10.21223/zhdp8m/rxwfnu
title: 02_Field_TVIT200412_AYMARA_Cooking.txt

description: :unav
DOI: 10.21223/zhdp8m/tuvgzv
title: TPHEN200412_AYMARA_Cooking.xls

description: :unav
DOI: 10.21223/zhdp8m/ewqkcy
title: 03_Material_list.txt

description: :unav
DOI: 10.21223/zhdp8m/fzhmvi
title: 04_Datadictionary.txt

description: :unav
DOI: 10.21223/zhdp8m/rz6tfx
title: 01_Field_TPHEN200412_AYMARA_Cooking.txt

description: :unav
DOI: 10.21223/zhdp8m
title: Dataset for: Analysis of Phenolic Compounds and Vitamin C in Potato Clones from Aymara - Huancavelica, 2004

description: An analysis of phenolic components and vitamin C was conducted on 14 potato clones under two conditions: raw and cooked samples, with three replications. The samples were sourced from Aymara, Huancavelica, in 2004.
DOI: 10.6084/m9.figshare.21904704
title: Direct and cost-effective method for histone isolation from cultured mammalian cells

description: Histones are an essential part of nucleosomes that regulate chromatin structure and function. Histone exchanges and modifications represent a scaffold for DNA transcription, repair, and replication. Studying histones and histone code is an important and fast-developing branch of epigenetic science. Here we propose a fast, efficient, and versatile assay for nucleosomal histone isolation from mammalian cells, without the use of acids or high salt solutions which are common for other histone isolation techniques. All components used in the protocol are common and inexpensive laboratory chemicals. The protocol has been evaluated on six commonly used cell lines and two animal tissue samples. The mild extraction conditions preserve delicate histone epigenetic changes, allowing its downstream analyses. We have demonstrated the assays’ successful application during changes in the transcriptional activity of histone genes, cell cycle transitions, and DNA-damaging conditions. Histone fractions, obtained by the protocol, can be used for further applications, such as electrophoresis, immunoblot, and mass spectrometry. Therefore, the new proposed nucleosomal histone isolation method is sensitive, specific, and suitable for downstream applications of various kinds.
DOI: 10.6084/m9.figshare.21904704.v1
title: Direct and cost-effective method for histone isolation from cultured mammalian cells

description: Histones are an essential part of nucleosomes that regulate chromatin structure and function. Histone exchanges and modifications represent a scaffold for DNA transcription, repair, and replication. Studying histones and histone code is an important and fast-developing branch of epigenetic science. Here we propose a fast, efficient, and versatile assay for nucleosomal histone isolation from mammalian cells, without the use of acids or high salt solutions which are common for other histone isolation techniques. All components used in the protocol are common and inexpensive laboratory chemicals. The protocol has been evaluated on six commonly used cell lines and two animal tissue samples. The mild extraction conditions preserve delicate histone epigenetic changes, allowing its downstream analyses. We have demonstrated the assays’ successful application during changes in the transcriptional activity of histone genes, cell cycle transitions, and DNA-damaging conditions. Histone fractions, obtained by the protocol, can be used for further applications, such as electrophoresis, immunoblot, and mass spectrometry. Therefore, the new proposed nucleosomal histone isolation method is sensitive, specific, and suitable for downstream applications of various kinds.
DOI: 10.6084/m9.figshare.15262161
title: Characterization of commercially available propolis products in Turkey based on individual phenolic compounds

description: The healing properties of propolis, such as antibacterial, antiviral, antifungal, anti-inflammatory, antitumoral, antioxidant, immunomodulatory, tissue regeneration, and anti-ulcer, are due to the high content of phenolic compounds (flavonoids and phenolic acids). Therefore, propolis can potentially be used for human consumption or even for medicinal purposes. This study aimed to analyze sixteen individual phenolic compounds that have beneficial effects, determine their concentrations in local and imported raw propolis and products consumed in Turkey, and evaluate the determined results for propolis quality. A total 91 propolis samples were collected from commercial raw propolis and propolis products, which were from different provinces of Turkey and different countries, sold and used in Turkey between 2015 and 2018. Sixteen phenolic compounds were analyzed by high-performance liquid chromatography with a diode array detector system. Regardless of their origin, the results indicated that the concentrations of individual phenolic compounds varied greatly within crude, ethanol-based, water-based, and propylene glycol propolis samples analysed. There were significant differences in some individual phenolic compounds between the local and imported samples (p &lt; 0.05). The current results showed that qualitative and quantitative analysis of commercial propolis samples for useful phenolic compounds are important and may provide quality control of propolis products.
DOI: 10.6084/m9.figshare.15262161.v1
title: Characterization of commercially available propolis products in Turkey based on individual phenolic compounds

description: The healing properties of propolis, such as antibacterial, antiviral, antifungal, anti-inflammatory, antitumoral, antioxidant, immunomodulatory, tissue regeneration, and anti-ulcer, are due to the high content of phenolic compounds (flavonoids and phenolic acids). Therefore, propolis can potentially be used for human consumption or even for medicinal purposes. This study aimed to analyze sixteen individual phenolic compounds that have beneficial effects, determine their concentrations in local and imported raw propolis and products consumed in Turkey, and evaluate the determined results for propolis quality. A total 91 propolis samples were collected from commercial raw propolis and propolis products, which were from different provinces of Turkey and different countries, sold and used in Turkey between 2015 and 2018. Sixteen phenolic compounds were analyzed by high-performance liquid chromatography with a diode array detector system. Regardless of their origin, the results indicated that the concentrations of individual phenolic compounds varied greatly within crude, ethanol-based, water-based, and propylene glycol propolis samples analysed. There were significant differences in some individual phenolic compounds between the local and imported samples (p &lt; 0.05). The current results showed that qualitative and quantitative analysis of commercial propolis samples for useful phenolic compounds are important and may provide quality control of propolis products.
DOI: 10.6084/m9.figshare.20382238
title: Impacts of pests and diseases on the decline of managed bees in Brazil: a beekeeper perspective

description: Brazilian beekeepers have experienced massive colony losses in recent years. Possible causes include bee pests and diseases, although there have been few relevant studies in the country and no official data are available. The aim of this work was to assess, from a beekeeper's perspective, the incidence of pests and diseases in colonies, and their impact on the decline of bee populations in Brazil. The research used two online questionnaires that included disease symptoms to facilitate participant identification of diseases. A total of 674 valid responses were obtained from beekeepers of <i>Apis mellifera</i> (49% of the total) and stingless bees (51%), distributed among 24 of the 27 Brazilian states. Most beekeepers observed pests (average 78.1%) and disease symptoms (65.5%) in their colonies. Based on beekeepers' responses, pests and diseases affect beehive losses in Brazil. Colony loss percentage for <i>A. mellifera</i> was higher in apiaries with ants (19.8% of colony losses) and wasps (46.7%) than in those without pests (p &lt; 0.001). While for stingless bees, meliponaries with robber bees (15.8% of colony losses), ants (14.7%), and phorid flies (12.5%) had higher colony losses than those without pests (p &lt; 0.001). Apiaries and meliponaries with worker bee deaths had higher losses than those without symptoms. The results also indicate that monitoring can reduce colony losses in apiaries and meliponaries that have some kind of pests and diseases. Using a beekeeper perspective, this study provides information about the impact that pests and pathogens have on bee losses that can be used to guide improved management practices.
DOI: 10.6084/m9.figshare.14578909
title: Biochemical composition and bioactivity analysis of sour honey samples from Nagaland, Northeast India

description: Sour honey is a natural product produced by <i>Trigona</i> species, which is a stingless bee. The distribution of this honey is very low because of the limited knowledge and has resulted in being less popular in terms of industrial production and quality standard. The objective of the present study is the biochemical and bioactivity analysis of 7 sour honeys (<i>Trigona</i> species) and one sweet honey collected from Nagaland, Northeast India. The biochemical and physiochemical screening of all samples was performed. The results of these tests are certainly encouraging its utilization as food supplements. In the antioxidant assay, the honey samples have exhibited 31-81% (P &gt; 0.05) of inhibition on DPPH as compared to standard ascorbic acid. Antibacterial test of the 7 sour honey samples has displayed a zone of inhibition ranging from 12 ± 0.21 mm to 17 ± 0.5 mm (P &gt; 0.05)against <i>Escherichia coli.</i> The catalase and oxidase tests conducted on the isolated microbial colonies from honey samples expressed positive results. In HPLC analysis, the retention time of the highest peak in all samples (3.79- 3.84) was identical to the retention time of standards (3.79-3.87) at dilution 10<sup>−5</sup> and a percentage of the area was nearly similar in all honey samples (11.93-14.84%) except SH<sub>6</sub> which has 19.92%. In brief, the beneficial effects of stingless bee honey in different contexts, such as biochemical components, physicochemical properties, quantification of sugars, antioxidant and antibacterial activity has experimented and all the results were positive. Further, these honey samples may have the probable potential for many nutraceuticals.
DOI: 10.6084/m9.figshare.14578909.v1
title: Biochemical composition and bioactivity analysis of sour honey samples from Nagaland, Northeast India

description: Sour honey is a natural product produced by <i>Trigona</i> species, which is a stingless bee. The distribution of this honey is very low because of the limited knowledge and has resulted in being less popular in terms of industrial production and quality standard. The objective of the present study is the biochemical and bioactivity analysis of 7 sour honeys (<i>Trigona</i> species) and one sweet honey collected from Nagaland, Northeast India. The biochemical and physiochemical screening of all samples was performed. The results of these tests are certainly encouraging its utilization as food supplements. In the antioxidant assay, the honey samples have exhibited 31-81% (P &gt; 0.05) of inhibition on DPPH as compared to standard ascorbic acid. Antibacterial test of the 7 sour honey samples has displayed a zone of inhibition ranging from 12 ± 0.21 mm to 17 ± 0.5 mm (P &gt; 0.05)against <i>Escherichia coli.</i> The catalase and oxidase tests conducted on the isolated microbial colonies from honey samples expressed positive results. In HPLC analysis, the retention time of the highest peak in all samples (3.79- 3.84) was identical to the retention time of standards (3.79-3.87) at dilution 10<sup>−5</sup> and a percentage of the area was nearly similar in all honey samples (11.93-14.84%) except SH<sub>6</sub> which has 19.92%. In brief, the beneficial effects of stingless bee honey in different contexts, such as biochemical components, physicochemical properties, quantification of sugars, antioxidant and antibacterial activity has experimented and all the results were positive. Further, these honey samples may have the probable potential for many nutraceuticals.
DOI: 10.6084/m9.figshare.17871917
title: African endemic stingless bees as an efficient alternative pollinator to honey bees in greenhouse cucumber (<i>Cucumis sativus</i> L)

description: The current honey bee decline necessitates the use of alternative native pollinators to ensure global food security. Here, we compared the pollination behaviour and efficiency of the African honey bee (<i>Apis mellifera</i>) and six African endemic Meliponini (<i>Meliponula bocandei, Dactylurina schmidti, Meliponula lendliana, Hypotrigona gribodoi, Meliponula ferruginea</i> and <i>Meliponula togoensis</i>) in a greenhouse with the non-parthenocarpic cucumber variety <i>Super Marketer</i>. Honey bees and <i>D. schmidti</i> started foraging on introduction in the greenhouse, while <i>M. lendliana</i> and <i>M. togoensis</i> showed the longest delay. In most species, foragers collected nectar and pollen, excepting <i>M. bocandei</i> that specialized in nectar collection, and <i>H. gribodoi</i> and <i>M. togoensis,</i> specialized in pollen collection. African honey bees visited flowers the shortest, while <i>H. gribodoi</i> and <i>D. schmidti</i> had a 2-fold probing time, on average. Most stingless bees species had a lower hive activity with fewer foragers encountered per hour than for honey bees, except <i>D. schmidti</i>. <i>M. bocandei, M. ferruginea</i>, <i>A. mellifera scutellata</i> and <i>H. gribodoi,</i> yielded a seed germination percentage of around 90%. <i>M. lendliana</i>, <i>M. togoensis</i> and <i>D. schmidti</i> yielded a much lower seed germination percentage around 30%, which indicates that the quality of pollination was remarkably lower by using these three species. The highest sugar content was recorded in fruits from flowers pollinated by <i>M. bocandei,</i> African honey bees<i>, D. schimdti</i> or <i>M. togoensis</i> with the same solid content as the gold standard method, i.e., hand cross-pollination. We found that <i>M. bocandei</i> was the most efficient cucumber pollinator of all species tested: because pollination by this species yielded the largest and heaviest fruits and the highest seed numbers.
DOI: 10.6084/m9.figshare.19793718.v1
title: The conflict between avian predators and domestic honey bees: a case study of European bee-eater (<i>Merops apiaster</i> L.) preying on the honey bee (<i>Apis mellifera</i> L.) in Cyprus

description: Bee-eaters are protected birds due to their contribution to the ecosystem, but at the same time, they inflict damages on bees which cause conflicts with beekeepers. This research aimed to study the impact of bee-eaters on the foraging activity of the honey bee (<i>Apis mellifera</i> L.) and the effectiveness of the applied control measures, proposing a solution for the coexistence of birds and bees. We discouraged bee-eaters by using different methods and testing their efficacy. The presence of bee-eaters around the apiary caused the reduction of honey bee foraging activity along with effects on brood rearing and temperature regulation of the hive. The so-far proposed measures to prevent bee-eaters' attacks in the apiaries were tested and found to have limited effect. Considering the role of honey bees in pollination and bee-eaters in the insect-bird food chains, we propose a harmonious coexistence of bees and birds. This strategy has been adopted by the beekeepers of Cyprus, who were committed to pursuing methods of limited protection of bees from the predatory birds, ensuring the survival of both components.
DOI: 10.6084/m9.figshare.20730936
title: Detection of honey bee viruses in apiaries in Southern Brazil through two standardized multiplex RT-PCR

description: Viruses are responsible for several diseases in animals and have also been linked to the drastic decrease in the number of bee hives worldwide. Considering the necessity to diagnose honey bee viruses, we developed and standardized two multiplex RT-PCRs. We evaluated the presence of six viruses described in Africanized <i>Apis mellifera</i> bees: Deformed wing virus (DWV), Sacbrood bee virus (SBV), Acute bee paralysis virus (ABPV), Israeli acute paralysis virus (IAPV), Black queen cell virus (BQCV), and Chronic bee paralysis virus (CBPV). Therefore, 164 samples of honey bees were collected in 27 apiaries of 12 municipalities in the states of Rio Grande do Sul and Santa Catarina, in southern Brazil. Among the 12 municipalities evaluated, five presented positive samples for BQCV, ABPV and DWV. Cases of co-infection by ABPV and BQCV and BQCV and DWV were observed in both states. In all apiaries with positive samples for viruses, <i>Varroa destructor</i> mite was reported or found. These results demonstrate the high occurrence of BQCV (36%) and the presence of ABPV (3%) and DWV (1%) in apiaries in Rio Grande do Sul, as well as in an apiary in Santa Catarina (75% BQCV and 12% DWV). The results confirm the circulation of these viruses in adult Africanized honey bees in southern Brazil, a factor that may be involved with the decline in the number of honey bees in this region of the country.
DOI: 10.6084/m9.figshare.17871917.v1
title: African endemic stingless bees as an efficient alternative pollinator to honey bees in greenhouse cucumber (<i>Cucumis sativus</i> L)

description: The current honey bee decline necessitates the use of alternative native pollinators to ensure global food security. Here, we compared the pollination behaviour and efficiency of the African honey bee (<i>Apis mellifera</i>) and six African endemic Meliponini (<i>Meliponula bocandei, Dactylurina schmidti, Meliponula lendliana, Hypotrigona gribodoi, Meliponula ferruginea</i> and <i>Meliponula togoensis</i>) in a greenhouse with the non-parthenocarpic cucumber variety <i>Super Marketer</i>. Honey bees and <i>D. schmidti</i> started foraging on introduction in the greenhouse, while <i>M. lendliana</i> and <i>M. togoensis</i> showed the longest delay. In most species, foragers collected nectar and pollen, excepting <i>M. bocandei</i> that specialized in nectar collection, and <i>H. gribodoi</i> and <i>M. togoensis,</i> specialized in pollen collection. African honey bees visited flowers the shortest, while <i>H. gribodoi</i> and <i>D. schmidti</i> had a 2-fold probing time, on average. Most stingless bees species had a lower hive activity with fewer foragers encountered per hour than for honey bees, except <i>D. schmidti</i>. <i>M. bocandei, M. ferruginea</i>, <i>A. mellifera scutellata</i> and <i>H. gribodoi,</i> yielded a seed germination percentage of around 90%. <i>M. lendliana</i>, <i>M. togoensis</i> and <i>D. schmidti</i> yielded a much lower seed germination percentage around 30%, which indicates that the quality of pollination was remarkably lower by using these three species. The highest sugar content was recorded in fruits from flowers pollinated by <i>M. bocandei,</i> African honey bees<i>, D. schimdti</i> or <i>M. togoensis</i> with the same solid content as the gold standard method, i.e., hand cross-pollination. We found that <i>M. bocandei</i> was the most efficient cucumber pollinator of all species tested: because pollination by this species yielded the largest and heaviest fruits and the highest seed numbers.
DOI: 10.6084/m9.figshare.20730936.v1
title: Detection of honey bee viruses in apiaries in Southern Brazil through two standardized multiplex RT-PCR

description: Viruses are responsible for several diseases in animals and have also been linked to the drastic decrease in the number of bee hives worldwide. Considering the necessity to diagnose honey bee viruses, we developed and standardized two multiplex RT-PCRs. We evaluated the presence of six viruses described in Africanized <i>Apis mellifera</i> bees: Deformed wing virus (DWV), Sacbrood bee virus (SBV), Acute bee paralysis virus (ABPV), Israeli acute paralysis virus (IAPV), Black queen cell virus (BQCV), and Chronic bee paralysis virus (CBPV). Therefore, 164 samples of honey bees were collected in 27 apiaries of 12 municipalities in the states of Rio Grande do Sul and Santa Catarina, in southern Brazil. Among the 12 municipalities evaluated, five presented positive samples for BQCV, ABPV and DWV. Cases of co-infection by ABPV and BQCV and BQCV and DWV were observed in both states. In all apiaries with positive samples for viruses, <i>Varroa destructor</i> mite was reported or found. These results demonstrate the high occurrence of BQCV (36%) and the presence of ABPV (3%) and DWV (1%) in apiaries in Rio Grande do Sul, as well as in an apiary in Santa Catarina (75% BQCV and 12% DWV). The results confirm the circulation of these viruses in adult Africanized honey bees in southern Brazil, a factor that may be involved with the decline in the number of honey bees in this region of the country.