cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19753289
title: Vertical distribution of orchid bees (Hymenoptera: Apidae: Euglossini) in an Amazon forest fragment

description: Euglossine bees are key pollinators of various plant species in the Neotropical region. Therefore, studying euglossine bee ecology is important for the development of conservation strategies. This study aimed to evaluate the vertical distribution of these bees in a “terra firme” forest fragment in the state of Rondônia, Brazil. The collections were carried out fortnightly from June 2017 to May 2018. The bees were sampled in the understory (1.5<b> </b>m in height) and the canopy (12<b> </b>m in height), using six aromatic compounds. A total of 9793 specimens were collected, representing 42 species of four genera. Statistically, the abundance of euglossines was higher in the understory, while the richness did not differ between strata. <i>Eulaema</i> Lepeletier, 1841 and <i>Exaerete</i> Hoffmannsegg, 1817 species were more abundant in the understory, while <i>Euglossa</i> Latreille, 1802 species were more abundant in the canopy stratum. <i>Eufriesea</i> Cockerell, 1908 species showed no difference in abundance between strata. In addition, nine new occurrences were recorded for the Rondônia state.
DOI: 10.13145/bacdive146034.20240510.9
title: Haemophilus intermedius subsp. intermedius

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.6084/m9.figshare.23509116
title: Choice of forage plants by the giant honey bee <i>Apis laboriosa</i> Smith in a captive condition outside their native geographic range

description: The giant honey bee, <i>Apis laboriosa</i>, is native to the central and eastern Himalayas, Hengduan, Arakan, Annamite and adjoining mountain ranges of Southeast Asia. The present work documented forage plants of <i>A. laboriosa</i> in a captive condition outside its natural geographic range for 09 months and tested their choice based on the abundance of the forager bees on flowers. The study was conducted by introducing a combless colony of <i>A. laboriosa</i> in a climate control greenhouse. A total of 68 plant species of 34 families were offered, out of which the foragers accepted the floral rewards from 28 species of 20 plant families. The foragers collected pollens and nectar from 10 and 18 plant species, respectively. A maximum number of foragers visited <i>Mesua ferrea</i> for pollen (16.94 ± 1.25 foragers/flower/hour) and <i>Indigofera</i> sp. for nectar (9.60 ± 1.24 foragers/flower/hour). Tukey’s Multiple Comparison test showed significant variation in the abundance of foragers on some plants. Analysis of variance showed no significant difference in the number of foragers at different time intervals of a day except for the nectar plants <i>Cucumis sativus</i> and <i>Indigofera</i> sp. The study also showed that the choice of the foragers was not associated with floral colours and plant family, but possibly dependent on some common cues of the forage plants resulting from convergent evolution. Foraging from plants of low altitudes also indicated that they can forage from plants outside their native geographic range.
DOI: 10.6084/m9.figshare.23509116.v1
title: Choice of forage plants by the giant honey bee <i>Apis laboriosa</i> Smith in a captive condition outside their native geographic range

description: The giant honey bee, <i>Apis laboriosa</i>, is native to the central and eastern Himalayas, Hengduan, Arakan, Annamite and adjoining mountain ranges of Southeast Asia. The present work documented forage plants of <i>A. laboriosa</i> in a captive condition outside its natural geographic range for 09 months and tested their choice based on the abundance of the forager bees on flowers. The study was conducted by introducing a combless colony of <i>A. laboriosa</i> in a climate control greenhouse. A total of 68 plant species of 34 families were offered, out of which the foragers accepted the floral rewards from 28 species of 20 plant families. The foragers collected pollens and nectar from 10 and 18 plant species, respectively. A maximum number of foragers visited <i>Mesua ferrea</i> for pollen (16.94 ± 1.25 foragers/flower/hour) and <i>Indigofera</i> sp. for nectar (9.60 ± 1.24 foragers/flower/hour). Tukey’s Multiple Comparison test showed significant variation in the abundance of foragers on some plants. Analysis of variance showed no significant difference in the number of foragers at different time intervals of a day except for the nectar plants <i>Cucumis sativus</i> and <i>Indigofera</i> sp. The study also showed that the choice of the foragers was not associated with floral colours and plant family, but possibly dependent on some common cues of the forage plants resulting from convergent evolution. Foraging from plants of low altitudes also indicated that they can forage from plants outside their native geographic range.
DOI: 10.6084/m9.figshare.19753289.v1
title: Vertical distribution of orchid bees (Hymenoptera: Apidae: Euglossini) in an Amazon forest fragment

description: Euglossine bees are key pollinators of various plant species in the Neotropical region. Therefore, studying euglossine bee ecology is important for the development of conservation strategies. This study aimed to evaluate the vertical distribution of these bees in a “terra firme” forest fragment in the state of Rondônia, Brazil. The collections were carried out fortnightly from June 2017 to May 2018. The bees were sampled in the understory (1.5<b> </b>m in height) and the canopy (12<b> </b>m in height), using six aromatic compounds. A total of 9793 specimens were collected, representing 42 species of four genera. Statistically, the abundance of euglossines was higher in the understory, while the richness did not differ between strata. <i>Eulaema</i> Lepeletier, 1841 and <i>Exaerete</i> Hoffmannsegg, 1817 species were more abundant in the understory, while <i>Euglossa</i> Latreille, 1802 species were more abundant in the canopy stratum. <i>Eufriesea</i> Cockerell, 1908 species showed no difference in abundance between strata. In addition, nine new occurrences were recorded for the Rondônia state.
DOI: 10.13145/bacdive146088.20240510.9
title: Aeromonas veronii

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.6084/m9.figshare.23301730
title: Parameters of the gross composition of propolis from Brazilian Meliponini

description: Propolis is a resinous and complex bee product containing resin derived from plants, beeswax, pollen and other minor constituents. The present study aimed to determine the parameters of the gross composition of propolis collected by nine stingless bee species from eight different Brazilian localities. The moisture content varied depending on the bee species and localities. Wax content enables us to distinguish between samples of <i>Melipona</i> and <i>Scaptotrigona</i>, samples of the former containing considerably less wax than samples of the latter genus. The ash content has an inverse trend, with samples of <i>Melipona</i> and <i>Scaptotrigona</i> having high and low ash content, respectively. The results indicated that <i>Melipona</i> species produce geopropolis (propolis containing aggregated soil material), while <i>Scaptotrigona</i> produces propolis devoid of soil, with the exception of <i>S. tubiba</i>. Most <i>Melipona</i> species collected sediments with high clay content. The size and color of ash crystals varied, mostly according to the locality of the meliponaries, although differences were observed between samples of the same species, growing in the same meliponary. Mineralogical analysis was relevant to determine that <i>M. flavolineata</i> from North Brazil aggregates river-bed soil sediments to the geopropolis.
DOI: 10.6084/m9.figshare.23301730.v1
title: Parameters of the gross composition of propolis from Brazilian Meliponini

description: Propolis is a resinous and complex bee product containing resin derived from plants, beeswax, pollen and other minor constituents. The present study aimed to determine the parameters of the gross composition of propolis collected by nine stingless bee species from eight different Brazilian localities. The moisture content varied depending on the bee species and localities. Wax content enables us to distinguish between samples of <i>Melipona</i> and <i>Scaptotrigona</i>, samples of the former containing considerably less wax than samples of the latter genus. The ash content has an inverse trend, with samples of <i>Melipona</i> and <i>Scaptotrigona</i> having high and low ash content, respectively. The results indicated that <i>Melipona</i> species produce geopropolis (propolis containing aggregated soil material), while <i>Scaptotrigona</i> produces propolis devoid of soil, with the exception of <i>S. tubiba</i>. Most <i>Melipona</i> species collected sediments with high clay content. The size and color of ash crystals varied, mostly according to the locality of the meliponaries, although differences were observed between samples of the same species, growing in the same meliponary. Mineralogical analysis was relevant to determine that <i>M. flavolineata</i> from North Brazil aggregates river-bed soil sediments to the geopropolis.
DOI: 10.6084/m9.figshare.23653785
title: <i>In vitro</i> maintenance of drones and development of a new software for sperm quality analysis facilitate the study of honey bee reproductive quality

description: This study aimed to develop a laboratory method that allows the <i>in vitro</i> maintenance of honey bee drones for several days while preserving their reproductive capacity and to create a new open-source software for the automatic analysis of their sperm quality. Three experiments were performed. The first experiment was designed to validate the new open-source software named CASABee for sperm quality assessment specifically designed for the honey bee. The software was able to identify motile and static spermatozoa with high precision. Results showed a high correlation between the results of sperm quality obtained both manually and by the CASABee system (0.95 and 0.96 for sperm motility and concentration, respectively, <i>p</i> &lt; 0.001). In the second and third experiments, the effect of <i>in vitro</i> maintenance of drones without attendant workers for four days on their ejaculatory capacity and sperm quality, respectively, was evaluated. Survival rate was 98.68%, 89.48%, 75.93%, and 60.97% on average on days 1, 2, 3, and 4 after capturing, respectively. A high proportion of the drones (80.37% on average) were able to ejaculate providing semen, and there were no significant differences in the ejaculatory capacity and sperm quality of drones on the different days of <i>in vitro</i> maintenance, except for sperm viability, which decreased slightly on day 4 (71.71% vs 82.8% on day 0, <i>p</i> &lt; 0.05). It was concluded that the new CASABee system and the method for laboratory maintenance of honey bee drones facilitate the study of reproduction in this species.
DOI: 10.6084/m9.figshare.23653785.v1
title: <i>In vitro</i> maintenance of drones and development of a new software for sperm quality analysis facilitate the study of honey bee reproductive quality

description: This study aimed to develop a laboratory method that allows the <i>in vitro</i> maintenance of honey bee drones for several days while preserving their reproductive capacity and to create a new open-source software for the automatic analysis of their sperm quality. Three experiments were performed. The first experiment was designed to validate the new open-source software named CASABee for sperm quality assessment specifically designed for the honey bee. The software was able to identify motile and static spermatozoa with high precision. Results showed a high correlation between the results of sperm quality obtained both manually and by the CASABee system (0.95 and 0.96 for sperm motility and concentration, respectively, <i>p</i> &lt; 0.001). In the second and third experiments, the effect of <i>in vitro</i> maintenance of drones without attendant workers for four days on their ejaculatory capacity and sperm quality, respectively, was evaluated. Survival rate was 98.68%, 89.48%, 75.93%, and 60.97% on average on days 1, 2, 3, and 4 after capturing, respectively. A high proportion of the drones (80.37% on average) were able to ejaculate providing semen, and there were no significant differences in the ejaculatory capacity and sperm quality of drones on the different days of <i>in vitro</i> maintenance, except for sperm viability, which decreased slightly on day 4 (71.71% vs 82.8% on day 0, <i>p</i> &lt; 0.05). It was concluded that the new CASABee system and the method for laboratory maintenance of honey bee drones facilitate the study of reproduction in this species.
DOI: 10.6084/m9.figshare.20060181
title: Impacts of a tailing dam break on the contamination of pollen and honey from the stingless bee <i>Tetragonisca angustula</i> (Apidae: Trigonini)

description: Metal and metalloid pollution are one of the main negative impacts of mining activities. Furthermore, the mining wastes are often stored in tailing dams, which are prone to failures and even total collapse. The Fundão tailing dam collapse in Brazil in 2015 flooded large areas of river floodplains below the dam. We analyzed the effect of the tailing deposition from the dam disaster on metal/metalloid levels in honey and pollen samples from the stingless bee <i>Tetragonisca angustula</i>. Pollen and honey samples were taken from the initial stretch of the impacted areas, as well as adjacent control areas and an additional external control area. In total, we analyzed 55 pollen samples and 53 honey samples, which were collected directly from the honey and pollen combs stored within the hives. The samples were wet acid digested in a microwave oven and the concentrations of the elements were determined by ICP-OES and ICP-MS. The metal/metalloid contents in honey and pollen samples were compared between the impacted and the non-impacted points. We found significantly higher concentrations of As, Ba, Cr, Mn, Ni and Zn, in honey and pollen samples, from some impacted points compared to the reference non-impacted ones. However, most concentrations we found were similar to those found in other works, including areas with mining activity in the Iron Quadrangle (Minas Gerais, Brazil). These results suggest that the tailing dram break, followed by deposition of the dam waste, contributed to a high concentration of some elements found in the pollen and honey samples at our study site.
DOI: 10.6084/m9.figshare.20060181.v1
title: Impacts of a tailing dam break on the contamination of pollen and honey from the stingless bee <i>Tetragonisca angustula</i> (Apidae: Trigonini)

description: Metal and metalloid pollution are one of the main negative impacts of mining activities. Furthermore, the mining wastes are often stored in tailing dams, which are prone to failures and even total collapse. The Fundão tailing dam collapse in Brazil in 2015 flooded large areas of river floodplains below the dam. We analyzed the effect of the tailing deposition from the dam disaster on metal/metalloid levels in honey and pollen samples from the stingless bee <i>Tetragonisca angustula</i>. Pollen and honey samples were taken from the initial stretch of the impacted areas, as well as adjacent control areas and an additional external control area. In total, we analyzed 55 pollen samples and 53 honey samples, which were collected directly from the honey and pollen combs stored within the hives. The samples were wet acid digested in a microwave oven and the concentrations of the elements were determined by ICP-OES and ICP-MS. The metal/metalloid contents in honey and pollen samples were compared between the impacted and the non-impacted points. We found significantly higher concentrations of As, Ba, Cr, Mn, Ni and Zn, in honey and pollen samples, from some impacted points compared to the reference non-impacted ones. However, most concentrations we found were similar to those found in other works, including areas with mining activity in the Iron Quadrangle (Minas Gerais, Brazil). These results suggest that the tailing dram break, followed by deposition of the dam waste, contributed to a high concentration of some elements found in the pollen and honey samples at our study site.
DOI: 10.6084/m9.figshare.23394586
title: A novel approach for improved honey identification and scientific definition: a case of buckwheat honey

description: This study aimed to provide insights into a novel honey screening and authentication approach. Chemometrics associated with a GC/MS instrumentation was applied to verify a total of 13 honey samples having different floral and geographical origin with an aim to detect buckwheat honey and discriminate it from honeys of other floral sources. Non-polar and semi-polar compounds were first extracted and then detected and semi-quantified employing a GC/MS device working in a full scan mode. Fingerprinting signals covering peaks that elute in the first 20 min, comprising 2000 scans of semi-polar compounds, were used as input datasets for unsupervised and supervised chemometric tools. This approach demonstrated excellent classification performances of honey samples according to the declared floral source, regardless of their geographical origin. The obtained results were compared with the outputs of a common melissopalynological procedure. Although exhibiting a high variability in the pollen content, samples declared as buckwheat honey demonstrated similar chemical profiles. The proposed non-targeted and semi-quantitative method showed to be rapid, unbiased, independent of tedious qualitative and quantitative determinations of eluting compounds, thus exhibiting a strong potential to be incorporated into data fusion procedures for honey authentication. Harmonising chemical methodologies with certain indications from pollen analysis to form core-data systems would undoubtedly improve the authentication protocols of honey floral sources.
DOI: 10.6084/m9.figshare.23394586.v1
title: A novel approach for improved honey identification and scientific definition: a case of buckwheat honey

description: This study aimed to provide insights into a novel honey screening and authentication approach. Chemometrics associated with a GC/MS instrumentation was applied to verify a total of 13 honey samples having different floral and geographical origin with an aim to detect buckwheat honey and discriminate it from honeys of other floral sources. Non-polar and semi-polar compounds were first extracted and then detected and semi-quantified employing a GC/MS device working in a full scan mode. Fingerprinting signals covering peaks that elute in the first 20 min, comprising 2000 scans of semi-polar compounds, were used as input datasets for unsupervised and supervised chemometric tools. This approach demonstrated excellent classification performances of honey samples according to the declared floral source, regardless of their geographical origin. The obtained results were compared with the outputs of a common melissopalynological procedure. Although exhibiting a high variability in the pollen content, samples declared as buckwheat honey demonstrated similar chemical profiles. The proposed non-targeted and semi-quantitative method showed to be rapid, unbiased, independent of tedious qualitative and quantitative determinations of eluting compounds, thus exhibiting a strong potential to be incorporated into data fusion procedures for honey authentication. Harmonising chemical methodologies with certain indications from pollen analysis to form core-data systems would undoubtedly improve the authentication protocols of honey floral sources.
DOI: 10.13145/bacdive146145.20240510.9
title: Bartonella vinsonii

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146183.20240510.9
title: Bergeyella zoohelcum

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146191.20240510.9
title: Campylobacter helveticus

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146192.20240510.9
title: Campylobacter helveticus

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146220.20240510.9
title: Streptococcus agalactiae

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146236.20240510.9
title: Campylobacter helveticus

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146246.20240510.9
title: Bifidobacterium longum

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146251.20240510.9
title: Legionella pneumophila

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146252.20240510.9
title: Legionella pneumophila

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146253.20240510.9
title: Legionella pneumophila

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146270.20240510.9
title: Weissella confusa

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146284.20240510.9
title: Neisseria weaveri

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146299.20240510.9
title: Haemophilus felis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146336.20240510.9
title: Weissella confusa

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146342.20240510.9
title: Weissella confusa

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146513.20240510.9
title: Bacillus licheniformis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146576.20240510.9
title: Rhodobacter capsulatus

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146618.20240510.9
title: Klebsiella pneumoniae subsp. rhinoscleromatis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive146619.20240510.9
title: Klebsiella pneumoniae subsp. rhinoscleromatis

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.