DOI: 10.5061/dryad.f0s23r8
title: Data from: Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity

description: The highly polymorphic human leukocyte antigen (HLA) class I molecules
 present peptide antigens to CD8+ T cells, inducing immunity against
 infections and cancers. Quality control mediated by peptide loading
 complex (PLC) components is expected to ensure the cell surface expression
 of stable peptide-HLA class I complexes. This is exemplified by
 HLA-B*08:01 in primary human lymphocytes, with both expression level and
 half-life at the high end of the measured HLA-B expression and stability
 hierarchies. Conversely, low expression on lymphocytes is measured for
 three HLA-B allotypes that bind peptides with proline at position 2, which
 are disfavored by the transporter associated with antigen processing.
 Surprisingly, these lymphocyte-specific expression and stability
 differences become reversed or altered in monocytes, which display larger
 intracellular pools of HLA class I than lymphocytes. Together, the
 findings indicate that allele and cell-dependent variations in antigen
 acquisition pathways influence HLA-B surface expression levels, half-lives
 and receptivity to exogenous antigens.
description: READMEdryad.f0s23r8
 README.rtfBeadsfcs files for all bead
 measurementsDonor 23 -
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 206W6/32 and Tapasin
 Intracellular StainingThis data corresponds to
 Figure 4 A and B. These are the fcs files for the flow cytometry
 experiments.W6-32_Tapasin_Intracellular-Staining.zipShannon Entropy calculationsThis data corresponds to Figure 7. It consists of the excel spreadsheet for the Shannon Entropy calculations.ShannonEntropy.zipDonor8: Bw4 StabilityData corresponding to HLA-Bw4 stability for Donor 8.Donor8.zipDonor14: Bw4 StabilityData corresponding to HLA-Bw4 stability for Donor 14.Donor14.zipDonor20: Bw4 StabilityData corresponding to HLA-Bw4 stability for Donor 20.Donor20.zipDonor62: Bw4 StabilityData corresponding to HLA-Bw4 stability for Donor 62.Donor62.zipDonor80: Bw4 StabilityThis data corresponds to the HLA-Bw4 stability data (flow cytommetry files) for Donor 80.Donor80.zipDonor64_Part1/2 Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 64.Donor64_Part1.zipDonor64_Part2/2: Bw4 StabilityThis data corresponds to the HLA-Bw4 stability data (flow cytommetry files) for Donor 64 (Part 2/2).Donor64_Part2.zipDonor156: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 156.Donor156.zipDonor91: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 91.Donor91.zipDonor111: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 111.Donor111.zipDonor121: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 121.Donor121.zipDonor126: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 126.Donor126.zipDonor128: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 128.Donor128.zipDonor142: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 142.Donor142.zipDonor155: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 155.Donor155.zipDonor194: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 194.Donor194.zipDonor198: Bw4 StabilityThis corresponds to the HLA-Bw4 stability flow cyvtometry fcs files for Donor 198.Donor198.zipDonor8: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 8.Donor8.zipDonor14: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 14.Donor14.zipDonor24_Part1/3: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 24.Donor24_Part1.zipDonor24_Part2/3: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 24.Donor24_Part2.zipDonor24_Part3/3: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 24.Donor24_Part3.zipDonor64_Part1/3: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 64.Donor64_Part1.zipDonor64_Part2/3: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 64.Donor64_Part2.zipDonor64_Part3/3: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 64.Donor64_Part3.zipDonor55: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 55.Donor55.zipDonor121: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 121.Donor121.zipDonor71: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 71.Donor71.zipDonor28_Part1/2: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 28.Donor28_Part1.zipDonor28_Part2/2: Bw6 StabilityThis corresponds to the HLA-Bw6 stability flow cytometry fcs files for Donor 28.Donor28_Part2.zipDonor31_Part1/3: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 31.Donor31_Part1.zipDonor31_Part2/3: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 31.Donor31_Part2.zipDonor31_Part3/3: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 31.Donor31_Part3.zipDonor91: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 91.Donor91.zipDonor111: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 111.Donor111.zipDonor94_Part1/2: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 94.Donor94_Part1.zipDonor94_Part2/2: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 94.Donor94_Part2.zipDonor215: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 215.Donor215.zipDonor206: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 206.Donor206.zipDonor198: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 198.Donor198.zipDonor141: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 141.Donor141.zipDonor120: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 120.Donor120.zipDonor124: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 124.Donor124.zipDonor128: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 128.Donor128.zipDonor131: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 131.Donor131.zipDonor137_Part1/3: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 137.Donor137_Part1.zipDonor137_Part2/3: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 137.Donor137_Part2.zipDonor137_Part3/3: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 137.Donor137_Part3.zipDonor178_Part1/2: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 178.Donor178_Part1.zipDonor178_Part2/2: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 178.Donor178_Part2.zipDonor187_Part1/2: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 187.Donor187_Part1.zipDonor187_Part2/2: Bw6 StabilityThis file corresponds to flow cytometry data for HLA-Bw6 stability of Donor 187.Donor187_Part2.zipDonor 64 ImageStreamImaging cytometry data for donor 64. Daf file format.Donor 94 ImageStreamImaging cytometry data for donor 94. Daf file format.Donor 237 ImageStreamImaging cytometry data for donor 237. Daf file format.Donor 14Data for Donor 14.Donor 24Data for donor 24Donor 28Data for donor 28Donor 31Data for donor 31Donor 64Data for donor 64Donor 71Data for donor 71Donor 94Data for donor 94Donor 137Data for donor 137Donor 141Data for donor 141Donor 178Data for donor 178Donor 187Data for donor 187Donor 198Data for donor 198