DOI: 10.6084/m9.figshare.28956857.v1
title: Live imaging and functional characterization of the avian hypoblast

description: Images and movies related to the paper "Live imaging and functional characterization of the avian hypoblast redefines the mechanisms of primitive streak induction".

- Live imaging of the hypoblast of trasngenic quail embryos during embryonic axis initiation (Figure 1).
- Photoconversion of mEOS2 in the epiblast and the hypoblast (Figure 2a).
- Simultaneaous live imaging of the epiblast and the hypoblast in a 2-color chimera (Figure 2b-d).
- Live imaging of embryos in which a filter was intercalated between the epiblast and the hypoblast (Figure 2e-g)
- HCR-RNA-FISH against NODAL in animals of different timings (Figure 3a-b)
- FOXA2 and NODAL RNA labelling (Figure 3c-d)
- CER1 and NODAL RNAs labelling (Figure 3e)
- Staining made in embryos in which an Activin-coated bead was grafted (Figure 4)
- Live imaging and staining in embryos in which the hypoblast was fully ablated (Figure 4)
- Live imaging and staining in embryos in which a portion of posterior hypoblast was ablated (Figure 4)
- Live imaging and staining in embryos in which posterior hypoblast was grafted anteriorly (Figure 4)
- Staining in SB505124-treated embryos (Figure 4)
- Staining in embryos in which 2h-anterior hypoblast cells were grafted onto a 2h-host (Figure 5)
- Staining in embryos in which 7h-anterior hypoblast was grafted onto the posterior side of a 7h-host (Figure 5)
- Timelapse movies and staining of embryos in which 7h-posterior epiblast was grafted onto the anterior side of a 2h-host (Figure 5)
- Proof of feasability of hypoblast dissection at 7h (Supplementary Fig.1c)
- Confirmation of hypoblast motion with a chimera in which only the hypoblast expresses fluorescent reporters (Supplementary Fig.2)
- Images of hypoblast cells labelled with H2B-GFP at 7.5h and 12h, and inference of hypoblast cell area using Voronoi tessellation (Supplementary Fig.3h)
- Phospho-Myosin and ZO1 staining (Supplementary Fig.4)
- NODAL and GDF1 RNAs labelling (Supplementary Fig.5)
- Timelapse movies of chimeras in which posterior and anterior hypoblast is labelled in 2 different colors (Supplementary Fig.6b)
- Backtracking of FOXA2-low/NODAL-high region (Supplementary Fig.6c)
- Live imaging and staining of chicken embryos in which the hypoblast was fully ablated (Supplementary Fig.7c)
- Live imaging and staining of embryos in which hypoblast was ablated and grafter back (Supplementary Fig.7c)
- Live imaging and staining of embryos in which an Activin-A coated (or control) bead has been grafted posteriorly after hypoblast removal (Supplementary Fig.8a)
- Live imaging and staining of embryos on which a control bead was deposited (Supplementary Fig.8b)
- Staining in embryos treated from 2 to 6h with SB-505124 (Supplementary Fig.8c)
- Staining of embryos in posterior hypoblast was grafted posteriorly at 2h (Supplementary Fig.9c)
- Timelapse movies and staining of embryos in which 7h-anterior epiblast was grafted onto the anterior side of a 2h-host (Supplementary Fig.9d)
- Staining of embryos in which 7h-anterior hypoblast was grafted onto the anterior side of a 2h-host (Supplementary Fig.10b)