DOI: 10.5061/dryad.41ns1rnss
title: Data from: Seed pelleting and storage effects on germination of Pennycress (<i>Thlaspi arvense</i> L.)

description: Pennycress (Thlaspi arvense L.) is an emerging bioenergy oilseed crop of
 interest to farmers in the Upper Midwestern United States. Improved lines
 with beneficial agronomic traits are being developed, including lines with
 reduced silicle shattering and altered seed coat characteristics, though
 planting and establishment is still challenging for this small‐seeded
 crop. A controlled‐environment experiment was conducted to assess the
 impact of seed treatment and pelleting on pennycress germination and
 seedling vigor of four pennycress lines (three black and one golden
 colored seed) following storage for 0, 1, 3, 9, and 12 months in warm or
 cold conditions (23°C or 10°C, respectively). Seeds were either treated
 with a gibberellin A4+7 (GA) soak (positive control), seed pelleting, or
 pelleting with GA added to the binder solution in addition to the
 untreated control (negative control). After each storage duration, seeds
 were germinated in darkness and counted daily for 7 days to obtain total
 germination. Vigor indices were calculated using changes in daily
 germination values. For black‐seeded lines, GA treatment increased
 germination over the untreated by 5%–75% and pelleting alone increased
 germination by 4%–30%, but only until 3 months of storage. The GA
 treatment did not increase germination of the golden‐seeded line, and
 pelleting decreased germination after 3 months of storage. The GA soak
 treatment was most effective at improving seed vigor indices compared with
 other treatments, while pelleting negatively affected vigor scores at 9
 and 12 months of storage. Similar effects on germination from treatment
 were observed under both warm and cold storage conditions. Results
 indicate seed treatment can benefit germination and vigor within 3 months
 of storage for black‐seeded lines but may negatively affect performance of
 golden‐seeded lines, or all pennycress seed types after 9 months of
 storage.
description: # Seed pelleting and storage effects on germination of Pennycress
 (*Thlaspi arvense* L.) Dataset DOI:
 [10.5061/dryad.41ns1rnss](10.5061/dryad.41ns1rnss) ## Description of the
 data and file structure This dataset contains information related to
 tested pennycress lines (4 in total) and the four seed treatments
 evaluated (untreated, a gibberellic acid soaking treatment, a pelleting
 treatment, and a pelleted treatment using gibberellic acid in the binder
 solution). Variables measured include storage environment temperature (23C
 or 10 °C) and duration of storage (0, 1, 3, 9, or 12 months). Collected
 data on each treatment includes cumulative germination over a seven-day
 period after the duration of storage was achieved. Data related to seed
 weight and diameter for each treatment is also included for the 0 month
 duration to provide baseline data related to seed characteristics. Missing
 data for a given variable within a plot is denoted solely with a “.” value
 as data was not or could not be collected in the specific instance. ###
 Files and variables #### File: Pellet_storage.csv ##### Variables * Row 1
 - Column Name: Column contains the descriptive titles for the first six
 rows of the worksheet, where each row describes a component of the data
 included within the spreadsheet. * Variety: One of the four varieties
 evaluated is named within this column. * Seed treatment: One of the four
 applied seed treatments is named in this column. * Block (Rep): This
 relates to when this experiment was performed in time. * Plate: Refers to
 individual units sampled at each timepoint within each block. * Storage
 Duration: This value in months is the time after treatment was applied
 when seeds were tested for germination. * Storage Temperature: Refers to
 temperature of the storage environment. Does not apply to the initial
 seedsets (0 months) because no storage had occurred at that time. * Total
 Germination day 3: Cumulative germination percentage on day 3 * Total
 Germination Day 4: Cumulative germination percentage on day 4 * Total
 Germination Day 5: Cumulative germination percentage on day 5 * Total
 Germination Day 6: Cumulative germination percentage on day 6 * Total
 Germination Day 7: Cumulative germination percentage on day 7 * Seed
 weight: Weight of 1000 seeds for each treatment * Seed Size: Weighted
 average diameter of seeds of each treatment in mm * Weight
 Difference: Change in weight of 5g of pelleted seeds in grams after
 shaking ## Access information Other publicly accessible locations of the
 data: * GEMS Informatics platform