DOI: 10.5061/dryad.vq2tn
title: Data from: Oxidative stress is related to both melanin- and carotenoid-based ornaments in the common yellowthroat

description: Male ornaments are hypothesized to signal the ability of males to produce
 an effective immune response without extensive oxidative stress and damage
 to DNA. We examined this hypothesis in male common yellowthroats
 (Geothlypis trichas), which have two ornaments, a black (eumelanin-based)
 facial mask and a yellow (carotenoid-based) bib. In our study population,
 only the black mask is sexually selected. As predicted by the oxidative
 stress hypothesis, males with larger black masks were more resistant to
 oxidative stress, as measured by an in vitro assay of the resistance of
 erythrocytes to haemolysis by free radicals. Furthermore, males with
 larger masks also tended to have lower levels of glutathione, which was
 predicted because glutathione inhibits eumelanin production. In contrast,
 mask size was not related to absolute levels of oxidative stress measured
 in the plasma. Although the yellow bib is not under sexual selection in
 our population, males with larger bibs and feathers with greater
 carotenoid chroma had lower levels of oxidative stress. The oxidative
 stress hypothesis was first proposed for carotenoid-based ornaments.
 However, our results suggest that, even in the same individuals,
 carotenoid and eumelanin-based plumage ornaments may both signal the
 ability of males to resist or manage oxidative stress.
description: KRL
 dataTime to hemolysis for
 50% of red blood cells in the KRL assay. File is tab-delimited text file
 with 11 variables (columns). The first row is the headers for each column
 and there are 39 subsequent rows of data.Variables names are:Band No Breed
 Experience (Experienced = known prior breeding experience; inexperienced =
 no known prior breeding experience on the main study area; blanks indicate
 unknown because the bird was captured off the main study area) Time to 50%
 hemolysis (the response variable) Jdate for KRL (date blood was sampled
 for KRL assay; 1=1 May) Tarsus (tarsus length of bird, mm) Mass (total
 body mass of bird, g) Mask (facial mask size of bird, mm^2) bib size (size
 of yellow throat patch, mm^2) Yellow brightness (see Methods) Ccar (see
 Methods) N replicates (number of different assays performed; see
 Methods)OxidativeStress &
 GSH dataTab delimited file with
 14 columns (variables) and 52 rows (top row contains the variable names).
 Variables are: Band Number (band number of individual bird [4 digit prefix
 and 5 digit suffix, after the decimal point]) Capture Date(1=1May) Breed
 Experience (see KRL file for description) Exper Group (see KRL file for
 description) Mask (see KRL file for description) bib size (see KRL file
 for description) Yellow Brightness (see KRL file for description) Ccar
 (see KRL file for description) Tarsus (mm) Weight pre (g, prior to bird
 being put in an aviary, if part of the LPS experiment) GSH pre (see
 Methods for GSH; "pre" refers to estimate prior to LPS
 experiment) ROMs/TAC_pre (see Methods; "pre" refers to estimate
 prior to LPS experiment) GSH post-pre (see Methods; "pre" refers
 to estimate prior to LPS experiment) ROMs/TAC post-pre (see Methods;
 "pre" refers to estimate prior to LPS
 experiment)