cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25549/webster-c100-38272
title: Inter-Agency Team Final Report, Los Angeles County Sheriff's Department, 1992

description: Inter-Agency Team Final Report, Appendix: Los Angeles County Sheriff's Department Miscellaneous materials collected by the Inter-Agency team regarding the role of the Los Angeles County Sheriff's Department in the response to the 1992 riots, 1992. PART OF SERIES: In addition to analyzing the 1992 riots as a whole, the Webster Commission also divided into several smaller task forces and study groups, each of which addressed one specific aspect of the civil disturbance. The series consists of reports and appendices produced by five of these groups: the Chronology Team, the Public Officials and Community Team, the LAPD Team, the Comparisons Study Group, and the Inter-Agency Team. Also included are two copies of Webster Scope, a report that interprets the riots and their cumulative effects primarily through graphs, charts, statistics, and figures, and several research memoranda that discuss the legality of several riot-response tactics. PART OF SUBSERIES: Materials related to a final report issued by the Webster Commission's Inter-Agency Team. which evaluated the collaboration between the LAPD and other law enforcement agencies in the advent of the 1992 riots. Included is a copy of the final report and the report's appendices, which are comprised of interviews, memoranda, statistical analyses, handbooks, and other miscellaneous documents.
DOI: 10.6084/m9.figshare.25714726.v2
title: Leaderless foot-and-mouth disease virus serotype O did not cause clinical disease and failed to establish a persistent infection in cattle

description: The foot-and-mouth disease virus (FMDV) Leader proteinase L<sup>pro</sup> inhibits host mRNA translation and blocks the interferon response which promotes viral survival. L<sup>pro</sup> is not required for viral replication in vitro but serotype A FMDV lacking L<sup>pro</sup> has been shown to be attenuated in cattle and pigs. However, it is not known, whether leaderless viruses can cause persistent infection in vivo after simulated natural infection and whether the attenuated phenotype is the same in other serotypes. We have generated an FMDV O/FRA/1/2001 variant lacking most of the L<sup>pro</sup> coding region (ΔLb). Cattle were inoculated intranasopharyngeally and observed for 35 days to determine if O FRA/1/2001 ΔLb is attenuated during the acute phase of infection and whether it can maintain a persistent infection in the upper respiratory tract. We found that although this leaderless virus can replicate in vitro in different cell lines, it is unable to establish an acute infection with vesicular lesions and viral shedding nor is it able to persistently infect bovine pharyngeal tissues.
DOI: 10.6084/m9.figshare.26426053
title: Inhibition of RAN attenuates influenza a virus replication and nucleoprotein nuclear export

description: Nuclear export of the viral ribonucleoprotein (vRNP) is a critical step in the influenza A virus (IAV) life cycle and may be an effective target for the development of anti-IAV drugs. The host factor ras-related nuclear protein (RAN) is known to participate in the life cycle of several viruses, but its role in influenza virus replication remains unknown. In the present study, we aimed to determine the function of RAN in influenza virus replication using different cell lines and subtype strains. We found that RAN is essential for the nuclear export of vRNP, as it enhances the binding affinity of XPO1 toward the viral nuclear export protein NS2. Depletion of RAN constrained the vRNP complex in the nucleus and attenuated the replication of various subtypes of influenza virus. Using in silico compound screening, we identified that bepotastine could dissociate the RAN-XPO1-vRNP trimeric complex and exhibit potent antiviral activity against influenza virus both <i>in vitro</i> and <i>in vivo</i>. This study demonstrates the important role of RAN in IAV replication and suggests its potential use as an antiviral target.
DOI: 10.6084/m9.figshare.26426053.v2
title: Inhibition of RAN attenuates influenza a virus replication and nucleoprotein nuclear export

description: Nuclear export of the viral ribonucleoprotein (vRNP) is a critical step in the influenza A virus (IAV) life cycle and may be an effective target for the development of anti-IAV drugs. The host factor ras-related nuclear protein (RAN) is known to participate in the life cycle of several viruses, but its role in influenza virus replication remains unknown. In the present study, we aimed to determine the function of RAN in influenza virus replication using different cell lines and subtype strains. We found that RAN is essential for the nuclear export of vRNP, as it enhances the binding affinity of XPO1 toward the viral nuclear export protein NS2. Depletion of RAN constrained the vRNP complex in the nucleus and attenuated the replication of various subtypes of influenza virus. Using in silico compound screening, we identified that bepotastine could dissociate the RAN-XPO1-vRNP trimeric complex and exhibit potent antiviral activity against influenza virus both <i>in vitro</i> and <i>in vivo</i>. This study demonstrates the important role of RAN in IAV replication and suggests its potential use as an antiviral target.
DOI: 10.25549/webster-c100-39602
title: Inter-agency team, final report, 1992

description: Inter-Agency Team Final Report, Appendix: Local police departments, materials collected by the Inter-Agency Team regarding the policies, procedures, and operations of several local police departments aside from the Los Angeles Police Department, 1992. PART OF SERIES: In addition to analyzing the 1992 riots as a whole, the Webster Commission also divided into several smaller task forces and study groups, each of which addressed one specific aspect of the civil disturbance. The series consists of reports and appendices produced by five of these groups: the Chronology Team, the Public Officials and Community Team, the LAPD Team, the Comparisons Study Group, and the Inter-Agency Team. Also included are two copies of Webster Scope, a report that interprets the riots and their cumulative effects primarily through graphs, charts, statistics, and figures, and several research memoranda that discuss the legality of several riot-response tactics. PART OF SUBSERIES: Materials related to a final report issued by the Webster Commission's Inter-Agency Team. which evaluated the collaboration between the LAPD and other law enforcement agencies in the advent of the 1992 riots. Included is a copy of the final report and the report's appendices, which are comprised of interviews, memoranda, statistical analyses, handbooks, and other miscellaneous documents.
DOI: 10.6084/m9.figshare.26354150
title: Genomic characterization of a reemerging Chikungunya outbreak in Kedougou, Southeastern Senegal, 2023

description: Chikungunya virus has caused millions of cases worldwide over the past 20 years, with recent outbreaks in Kedougou region in the southeastern Senegal, West Africa. Genomic characterization highlights that an ongoing epidemic in Kedougou in 2023 is not due to an introduction event but caused by the re-emergence of an endemic strain evolving linearly in a sylvatic context.
DOI: 10.6084/m9.figshare.26426053.v1
title: Inhibition of RAN Attenuates Influenza A Virus Replication and Nucleoprotein Nuclear Export

description: Nuclear export of the viral ribonucleoprotein (vRNP) is a critical step in the influenza A virus (IAV) life cycle and may be an effective target for the development of anti- IAV drugs. The host factor ras-related nuclear protein (RAN) is known to participates in the life cycle of several viruses, but its role in influenza virus replication remains unknown. In the present study, we aimed to determine the function of RAN in influenza virus replication using different cell lines and subtype strains. We found that RAN is essential for the nuclear export of vRNP, as it enhances the binding affinity of XPO1 toward the viral nuclear export protein NS2. Depletion of RAN constrained the vRNP complex in the nucleus and attenuated the replication of various subtypes of influenza virus. Using in silico compound screening, we identified bepotastine could dissociate the RAN-XPO1-vRNP trimeric complex and exhibit potent antiviral activity against influenza virus both <i>in vitro</i> and <i>in vivo</i>. This study demonstrates the important role of RAN in IAV replication and suggests its potential use as an antiviral agent.
DOI: 10.6084/m9.figshare.26954340
title: Implications of accumulation of clonally expanded and senescent CD4<sup>+</sup>GNLY<sup>+</sup> T cells in immunological non-responders of HIV-1 infection

description: Increased CD4<sup>+</sup>GNLY<sup>+</sup> T cells have been confirmed to be inversely associated with CD4<sup>+</sup> T cell count in immunological non-responders (INRs), however, the underlying mechanisms are unknown. This study aimed to elucidate the characteristics of CD4<sup>+</sup>GNLY<sup>+</sup> T cells and their relationship with immune restoration. Single-cell RNA sequencing, single-cell TCR sequencing, and flow cytometry were used to analyze the frequency, phenotypes, and function of CD4<sup>+</sup>GNLY<sup>+</sup> T cells. Moreover, Enzyme linked immunosorbent assay was performed to detect plasma cytokines production in patients. CD4<sup>+</sup>GNLY<sup>+</sup> T cells were found to be highly clonally expanded, characterized by higher levels of cytotoxicity, senescence, P24, and HIV-1 DNA than CD4<sup>+</sup>GNLY<sup>−</sup> T cells. Additionally, the frequency of CD4<sup>+</sup>GNLY<sup>+</sup> T cells increased after ART, and further increased in INRs, and were positively associated with the antiretroviral therapy duration in INR. Furthermore, increased IL-15 levels in INRs positively correlated with the frequency and senescence of CD4<sup>+</sup>GNLY<sup>+</sup> T cells, suggesting that CD4<sup>+</sup>GNLY<sup>+</sup> T cells may provide new insights for understanding the poor immune reconstitution of INRs. In conclusion, increased, highly clonally expanded, and senescent CD4<sup>+</sup>GNLY<sup>+</sup> T cells may contribute to poor immune reconstitution in HIV-1 infection.
DOI: 10.25549/webster-c100-32371
title: Michael Rogers, interview, 1992-06-30

description: Interview of Michale Rogers, Sergeant for the Los Angeles Police Department's Pacific Division, about his recollection of the civil disturbance, the preparedness of the Los Angeles Police Department to handle a major disturbance, and the adequacy of the Pacific Division's response to the violence and unrest, 1992 June 30. PART OF SERIES: After the violence had subsided, the Webster Commission met of a variety of public officials, law enforcement personnel, activists, and community leaders to discuss the rioting, its root causes, and the adequacy of the LAPD's response. The series includes summaries of these meetings, including questions and comments along of the interviewers' own mental impressions, conclusions, and opinions. Materials are arranged in alphabetical order, according to the last name of the interviewee.
DOI: 10.6084/m9.figshare.24720416
title: Viral diversity in wild and urban rodents of Yunnan Province, China

description: Rodents represent over 40% of known mammal species and are found in various terrestrial habitats. They are significant reservoirs for zoonotic viruses, including harmful pathogens such as arenaviruses and hantaviruses, yet knowledge of their hosts and distributions is limited. Therefore, characterizing the virome profile in these animals is invaluable for outbreak preparedness, especially in potential hotspots of mammal diversity. This study included 681 organs from 124 rodents and one Chinese tree shrew collected from Yunnan Province, China, during 2020-2021. Metagenomic analysis revealed unique features of mammalian viruses in rodent organs across habitats with varying human disturbances. <i>R. tanezumi</i> in locations with high anthropogenic disturbance exhibited the highest mammal viral diversity, with spleen and lung samples showing the highest diversities for these viruses at the organ level. Mammal viral diversity for both commensal and non-commensal rats was identified to positively correlate with landscape disturbance. Some virus families were associated with particular organs or host species, suggesting tropism for these pathogens. Notably, known and novel viral species that are likely to infect humans were identified. <i>R. tanezumi</i> was identified as a reservoir and carrier for various zoonotic viruses, including porcine bocavirus, hantavirus, cardiovirus, and lyssavirus. These findings highlight the influence of rodent community composition and anthropogenic activities on diverse virome profiles, with <i>R. tanezumi</i> as an important reservoir for zoonotic viruses.
DOI: 10.6084/m9.figshare.25379341
title: Tracing histoplasmosis genomic epidemiology and species occurrence across the USA

description: Histoplasmosis is an endemic mycosis in North America frequently reported along the Ohio and Mississippi River Valleys, although autochthonous cases occur in non-endemic areas. In the United States, the disease is provoked by two genetically distinct clades of <i>Histoplasma capsulatum sensu lato</i>, <i>Histoplasma mississippiense</i> (Nam1) and <i>H. ohiense</i> (Nam2). To bridge the molecular epidemiological gap, we genotyped 93 <i>Histoplasma</i> isolates (62 novel genomes) including clinical, environmental, and veterinarian samples from a broader geographical range by whole-genome sequencing, followed by evolutionary and species niche modelling analyses. We show that histoplasmosis is caused by two major lineages, <i>H. ohiense</i> and <i>H. mississippiense</i>; with sporadic cases caused by <i>H. suramericanum</i> in California and Texas. While <i>H. ohiense</i> is prevalent in eastern states, <i>H. mississipiense</i> was found to be prevalent in the central and western portions of the United States, but also geographically overlapping in some areas suggesting that these species might co-occur. Species Niche Modelling revealed that <i>H. ohiense</i> thrives in places with warmer and drier conditions, while <i>H. mississippiense</i> is endemic to areas with cooler temperatures and more precipitation. In addition, we predicted multiple areas of secondary contact zones where the two species co-occur, potentially facilitating gene exchange and hybridization. This study provides the most comprehensive understanding of the genomic epidemiology of histoplasmosis in the USA and lays a blueprint for the study of invasive fungal diseases.
DOI: 10.6084/m9.figshare.25379341.v1
title: Tracing histoplasmosis genomic epidemiology and species occurrence across the USA

description: Histoplasmosis is an endemic mycosis in North America frequently reported along the Ohio and Mississippi River Valleys, although autochthonous cases occur in non-endemic areas. In the United States, the disease is provoked by two genetically distinct clades of <i>Histoplasma capsulatum sensu lato</i>, <i>Histoplasma mississippiense</i> (Nam1) and <i>H. ohiense</i> (Nam2). To bridge the molecular epidemiological gap, we genotyped 93 <i>Histoplasma</i> isolates (62 novel genomes) including clinical, environmental, and veterinarian samples from a broader geographical range by whole-genome sequencing, followed by evolutionary and species niche modelling analyses. We show that histoplasmosis is caused by two major lineages, <i>H. ohiense</i> and <i>H. mississippiense</i>; with sporadic cases caused by <i>H. suramericanum</i> in California and Texas. While <i>H. ohiense</i> is prevalent in eastern states, <i>H. mississipiense</i> was found to be prevalent in the central and western portions of the United States, but also geographically overlapping in some areas suggesting that these species might co-occur. Species Niche Modelling revealed that <i>H. ohiense</i> thrives in places with warmer and drier conditions, while <i>H. mississippiense</i> is endemic to areas with cooler temperatures and more precipitation. In addition, we predicted multiple areas of secondary contact zones where the two species co-occur, potentially facilitating gene exchange and hybridization. This study provides the most comprehensive understanding of the genomic epidemiology of histoplasmosis in the USA and lays a blueprint for the study of invasive fungal diseases.
DOI: 10.6084/m9.figshare.26954340.v2
title: Implications of accumulation of clonally expanded and senescent CD4<sup>+</sup>GNLY<sup>+</sup> T cells in immunological non-responders of HIV-1 infection

description: Increased CD4<sup>+</sup>GNLY<sup>+</sup> T cells have been confirmed to be inversely associated with CD4<sup>+</sup> T cell count in immunological non-responders (INRs), however, the underlying mechanisms are unknown. This study aimed to elucidate the characteristics of CD4<sup>+</sup>GNLY<sup>+</sup> T cells and their relationship with immune restoration. Single-cell RNA sequencing, single-cell TCR sequencing, and flow cytometry were used to analyze the frequency, phenotypes, and function of CD4<sup>+</sup>GNLY<sup>+</sup> T cells. Moreover, Enzyme linked immunosorbent assay was performed to detect plasma cytokines production in patients. CD4<sup>+</sup>GNLY<sup>+</sup> T cells were found to be highly clonally expanded, characterized by higher levels of cytotoxicity, senescence, P24, and HIV-1 DNA than CD4<sup>+</sup>GNLY<sup>−</sup> T cells. Additionally, the frequency of CD4<sup>+</sup>GNLY<sup>+</sup> T cells increased after ART, and further increased in INRs, and were positively associated with the antiretroviral therapy duration in INR. Furthermore, increased IL-15 levels in INRs positively correlated with the frequency and senescence of CD4<sup>+</sup>GNLY<sup>+</sup> T cells, suggesting that CD4<sup>+</sup>GNLY<sup>+</sup> T cells may provide new insights for understanding the poor immune reconstitution of INRs. In conclusion, increased, highly clonally expanded, and senescent CD4<sup>+</sup>GNLY<sup>+</sup> T cells may contribute to poor immune reconstitution in HIV-1 infection.
DOI: 10.6084/m9.figshare.26946026
title: Genetic microevolution of clinical <i>Candida auris</i> with reduced Amphotericin B sensitivity in China

description: The global rate of Amphotericin B (AmB) resistance in <i>Candida auris</i> has surpassed 12%. However, there is limited data on available clinical treatments and microevolutionary analyses concerning reduced AmB sensitivity. In this study, we collected 18 <i>C. auris</i> isolates from five patients between 2019 and 2022. We employed clinical data mining, genomic, and transcriptomic analyses to identify genetic evolutionary features linked to reduced AmB sensitivity in these isolates during clinical treatment. We identified six isolates with a minimum inhibitory concentration (MIC) of AmB below 0.5 µg/mL (AmB<sup>0.5</sup>) and 12 isolates with an AmB-MIC of 1 µg/mL (AmB<sup>1</sup>) or ≥ 2 µg/mL (AmB<sup>2</sup>). All five patients received 24-hour AmB (5 mg/L) bladder irrigation treatment. Evolutionary analyses revealed an <i>ERG3</i> (c923t) mutation in AmB<sup>1</sup> <i>C. auris</i>. Additionally, AmB<sup>2</sup> <i>C. auris</i> was found to contain a t2831c mutation in the <i>RAD2</i> gene. In the AmB<sup>1</sup> group, membrane lipid-related gene expression (<i>ERG1, ERG2, ERG13,</i> and <i>ERG24</i>) was upregulated, while in the AmB<sup>2</sup> group, expression of DNA-related genes (e.g. <i>DNA2</i> and <i>PRI1</i>) was up-regulated. In a series of <i>C.auris</i> strains with reduced susceptibility to AmB, five key genes were identified: two upregulated (<i>IFF9</i> and <i>PGA6)</i> and three downregulated (<i>HGT7, HGT13,</i>and <i>PRI32)</i>. In this study, we demonstrate the microevolution of reduced AmB sensitivity in vivo and further elucidate the relationship between reduced AmB sensitivity and low-concentration AmB bladder irrigation. These findings offer new insights into potential antifungal drug targets and clinical markers for the “super fungus”, <i>C. auris</i>.
DOI: 10.6084/m9.figshare.26954340.v1
title: Implications of Accumulation of Clonally Expanded and Senescent CD4 <sup>+ </sup>GNLY<sup>+</sup> T Cells in Immunological Non-responders of HIV-1 infection

description: Increased CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells have been confirmed to be inversely associated with CD4<sup>+</sup> T cell count in immunological non-responders (INRs), however, the underlying mechanisms are unknown. This study aimed to elucidate the characteristics of CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells and their relationship with immune restoration. Single-cell RNA sequencing, single-cell TCR sequencing, and flow cytometry were used to analyze the frequency, phenotypes, and function of CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells. Moreover, Enzyme linked immunosorbent assay was performed to detect plasma cytokines production in patients. CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells were found to be highly clonally expanded, characterized by higher levels of cytotoxicity, senescence, P24, and HIV-1 DNA than CD4 <sup>+ </sup>GNLY<sup>-</sup> T cells. Additionally, the frequency of CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells increased after ART, and further increased in INRs, and were positively associated with the antiretroviral therapy duration in INR. Furthermore, increased IL-15 levels in INRs positively correlated with the frequency and senescence of CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells, suggesting that CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells may provide new insights for understanding the poor immune reconstitution of INRs. In conclusion, increased, highly clonally expanded, and senescent CD4 <sup>+ </sup>GNLY<sup>+</sup> T cells may contribute to poor immune reconstitution in HIV-1 infection.
DOI: 10.25549/webster-c100-39468
title: Inter-agency team, final report, 1992

description: Inter-Agency Team Final Report, Appendix: Local police departments, materials collected by the Inter-Agency Team regarding the policies, procedures, and operations of several local police departments aside from the Los Angeles Police Department, 1992. PART OF SERIES: In addition to analyzing the 1992 riots as a whole, the Webster Commission also divided into several smaller task forces and study groups, each of which addressed one specific aspect of the civil disturbance. The series consists of reports and appendices produced by five of these groups: the Chronology Team, the Public Officials and Community Team, the LAPD Team, the Comparisons Study Group, and the Inter-Agency Team. Also included are two copies of Webster Scope, a report that interprets the riots and their cumulative effects primarily through graphs, charts, statistics, and figures, and several research memoranda that discuss the legality of several riot-response tactics. PART OF SUBSERIES: Materials related to a final report issued by the Webster Commission's Inter-Agency Team. which evaluated the collaboration between the LAPD and other law enforcement agencies in the advent of the 1992 riots. Included is a copy of the final report and the report's appendices, which are comprised of interviews, memoranda, statistical analyses, handbooks, and other miscellaneous documents.
DOI: 10.5281/zenodo.14292526
title: RIVOJLANGAN DAVLATLAR TIBBIY SUG'URTA TIZIMINI O`ZBEKISTONGA JORIY ETISH XUSUSIYATLARINI BAXOLASH
DOI: 10.5281/zenodo.14292527
title: RIVOJLANGAN DAVLATLAR TIBBIY SUG'URTA TIZIMINI O`ZBEKISTONGA JORIY ETISH XUSUSIYATLARINI BAXOLASH