DOI: 10.5061/dryad.1g1jwsv5m
title: Fractions of soil phosphorus mediated by rhizospheric phoD-harboring bacteria of deep-rooted desert species are determined by fine-root traits

description: Soil phosphorus (P) availability is a crucial factor determining primary
 productivity in terrestrial ecosystems. Plant functional traits and
 microbes under P-deficient conditions can respond positively to increase
 soil P bioavailability. Whether and/or how the fine-root traits (FRTs) of
 deep-rooted desert species affect the rhizosphere and bulk-soil community
 of phoD-harboring bacteria and thus improve the availability of soil P,
 however, remains unclear. We conducted a three-year artificial
 outdoor pot experiment of P supply using Alhagi sparsifolia Shap.
 (hereafter Alhagi) to address this gap. Fine-root samples from one- and
 three-year-old Alhagi seedlings and samples of the rhizospheres and bulk
 soil were collected. High-throughput sequencing, sequential extraction,
 and root system scanning were used to determine soil phoD-harboring
 bacteria community, Hedley-P fractions, and the FRTs. Fine-root
 surface area (RSA), specific root length, foliar Mn concentration
 (indicating the quantities of root carboxylates that are released), and
 acid phosphatase (APase) activity were significantly higher in the no-P
 supply compared to the high-P supply conditions. APase activity was
 significantly higher by 27%, but the foliar Mn concentration was
 remarkably lower by 26%, in the three than the one-year-old seedlings. The
 rhizospheric concentrations of labile P, moderately labile P, inorganic P,
 and organic P in the no-P supply condition were 5, 11, 10, and 21% higher,
 respectively, in the three- than the one-year-old seedlings. RSA and the
 foliar Mn concentration were dominant root predictors for the rhizospheric
 phoD-harboring bacteria community for the one-year-old seedlings, whereas
 fine-root P concentration was the dominant root predictor for the
 rhizospheric and bulk-soil phoD-harboring bacteria communities for the
 three-year-old seedlings. Soil-water content, as the most dominant soil
 factor driving the variation of phoD-harboring bacteria community, notably
 could not be ignored. FRTs were the main factors that directly and
 positively determined the rhizospheric phoD-harboring bacteria
 community and thus influenced soil P availability, but bulk-soil
 phoD-harboring bacteria community were dominated by inorganic P
 concentration. The importance of fine-root morphological traits to soil P
 availability gradually increased as the plants grew. Overall, our
 results emphasize the significance of rhizospheric phoD-harboring
 bacteria determined by the effect of FRTs on the bioavailability of soil
 P.
description: # Fractions of soil phosphorus mediated by rhizospheric phoD-harboring
 bacteria of deep-rooted desert species are determined by fine-root traits
 [https://doi.org/10.5061/dryad.1g1jwsv5m](https://doi.org/10.5061/dryad.1g1jwsv5m) ## Description of the data and file structure This experiment was conducted at the Cele Nation Station of Observation and Research for Desert-Grassland Ecosystems, situated in the ecotone between the Qira Oasis and the Taklamakan Desert in Xinjiang Province, China. PhoD data were derived from rhizosphere and bulk soil samples collected from a pot experiment with one year and three years of phosphorus treatment. ### Files and variables ###### phoD.zip Contains 128 fastq (fq) files. Naming conventions: * One-year: ZTPSN21HD180-A * Three-year: ZTPSN21HD180-B - P treatment - CK: No P supply - T1: 1 g P m–2 year–1 - T2: 3 g P m–2 year–1 - T3: 5 g P m–2 year–1 Fastq files: ZTPSN21HD180-A_CK_R_R.fq ZTPSN21HD180-A_T1_R_R.fq ZTPSN21HD180-A_T2_R_R.fq ZTPSN21HD180-A_T3_R_R.fq ZTPSN21HD180-A_CK_nR_R.fq ZTPSN21HD180-A_T1_nR_R.fq ZTPSN21HD180-A_T2_nR_R.fq ZTPSN21HD180-A_T3_nR_R.fq ZTPSN21HD180-B_CK_R_R.fq ZTPSN21HD180-B_T1_R_R.fq ZTPSN21HD180-B_T2_R_R.fq ZTPSN21HD180-B_T3_R_R.fq ZTPSN21HD180-B_CK_nR_R.fq ZTPSN21HD180-B_T1_nR_R.fq ZTPSN21HD180-B_T2_nR_R.fq ZTPSN21HD180-B_T3_nR_R.fq