cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17031/66584fab3dcfe
title: 2012-2013 University of Southampton Poole Harbour Crassostrea gigas transect and settlement panel records
title: 2013 University of Southampton Poole Harbour Magallana gigas transect and settlement panel records

description: The work carried out so far highlights Poole Harbour as being an anomaly in the apparent steady progressive development of Pacific oyster populations around the coast, in that there is an obvious source of larvae but little successful settlement. The mechanisms which are ‘dampening down’ expected settlement appear to include at least one physical factor but it is likely that this is moderated by biological factors such as competition for settlement space and predation. Further work is required to establish which biological factors may be modifying recruitment success and whether recruitment is moderated during the larval stage or post metamorphosis. if the dominant controlling factor(s) act before oyster settlement it is most likely water temperature that controls the naturalised population, however top-down control by predators could be significant if oysters are surviving metamorphosis.
description: Part of MSFD NNS baseline data collation project (Hughes, E., Wood, C.A., Arnold, M., Bishop, J.D.D., Griffiths, C., Sewell, J., and Lear, D. (2016) Collating baseline invasive non-indigenous species data for MSFD reporting. Defra report ME5223. 59pp.). Subset of a dataset as records were only pulled for a selection of INNS.
DOI: 10.15468/dl.ve37yp
title: Occurrence Download

description: A dataset containing 23316076 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Bacteria"
 ]
}

 The dataset includes 23316076 records from 2189 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032129-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15143810
title: ТабΛица 1 Морфометрические признаки Viscosia orientalis sp. nov. Table 1 Morphometric features of Viscosia orientalis sp. nov in Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam

description: ТабΛица 1 Морфометрические признаки Viscosia orientalis sp. nov. Table 1 Morphometric features of Viscosia orientalis sp. nov
ПризнакГоΛотип самецПаратипы 2 самца 2 самки 1 2 1 2ΔΛина теΛа, мкм1122991111311571160a2017201719b4,75,15,04,34,4c19,315,719,919,318,4c ʹ2,83,02,82,52,6V, %———51,452,1Ширина теΛа, мкм:на уровне гоΛовных щетинок1718171719в его среΑнем отΑеΛе5460566860на уровне ануса иΛи кΛоаки2019202424ΔΛина, мкм:гоΛовных щетинок1,51,51,51,51,5стомы1920181919фаринкса241194224272264хвоста5863566063спикуΛ (по Αуге)343634——Расстояние, мкм:от конца фаринкса Αо вуΛьвы———323340от конца фаринкса Αо кΛоаки823734833——от вуΛьвы Αо ануса———502493
description: Published as part of Gagarin, V. G., 2020, Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam, pp. 30 in Amurian Zoological Journal XII (1) on page 30, DOI: 10.33910/2686-9519-2020-12-1-26-42, http://zenodo.org/record/11088743
DOI: 10.5281/zenodo.15143811
title: ТабΛица 1 Морфометрические признаки Viscosia orientalis sp. nov. Table 1 Morphometric features of Viscosia orientalis sp. nov in Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam

description: ТабΛица 1 Морфометрические признаки Viscosia orientalis sp. nov. Table 1 Morphometric features of Viscosia orientalis sp. nov
ПризнакГоΛотип самецПаратипы 2 самца 2 самки 1 2 1 2ΔΛина теΛа, мкм1122991111311571160a2017201719b4,75,15,04,34,4c19,315,719,919,318,4c ʹ2,83,02,82,52,6V, %———51,452,1Ширина теΛа, мкм:на уровне гоΛовных щетинок1718171719в его среΑнем отΑеΛе5460566860на уровне ануса иΛи кΛоаки2019202424ΔΛина, мкм:гоΛовных щетинок1,51,51,51,51,5стомы1920181919фаринкса241194224272264хвоста5863566063спикуΛ (по Αуге)343634——Расстояние, мкм:от конца фаринкса Αо вуΛьвы———323340от конца фаринкса Αо кΛоаки823734833——от вуΛьвы Αо ануса———502493
description: Published as part of Gagarin, V. G., 2020, Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam, pp. 30 in Amurian Zoological Journal XII (1) on page 30, DOI: 10.33910/2686-9519-2020-12-1-26-42, http://zenodo.org/record/11088743
DOI: 10.5281/zenodo.15143812
title: ТабΛица 2 Основные морфоΛогические признаки самцов ваΛиΑных виΑов роΑа Viscosia, имеющих ΑΛину теΛа в преΑеΛах 1 – 2 мм Table 2 Basic morphological features of males of the genus Viscosia valid species with body length of 1 – 2 mm in Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam

description: ТабΛица 2 Основные морфоΛогические признаки самцов ваΛиΑных виΑов роΑа Viscosia, имеющих ΑΛину теΛа в преΑеΛах 1–2 мм Table 2 Basic morphological features of males of the genus Viscosia valid species with body length of 1–2 mm
ВиΑΑитературный источникnLabcc ʹФорма и ΑΛина внешних губных сенсиΛΛ, мкмΔΛина стомы, мкмΔΛина спикуΛ, мкмbandaensisKreis 19324♂♂1389–171541–484,8–5,112,8–13,86,0–6,5пап.2318–20bayensisKeppner 19876♂♂1630–182041–485,217,35,6щет. 9–1125–2943–50brachylaimoidesChitwood 19373♂♂1700–254027–396,2–7,414,2–25,03,0щет. 2,0??epapillosaПΛатонова 19711♂1705515,320,74,9щет. 6,01629erasmiFurstenberg, Vincx 19891♂1905605,715,95,0щет. 5,02332glabraBastian 1865; Platt, Warwick 1983♂♂1600–210042–705,3–6,37,5–11,010–13пап.23–2527longicaudatoidesНгуен Ву Тхань, Гагарин, 20132♂♂1348–143253–544,8–5,48,3–9,010,1–10,8щет. 2,3–3,017–1818–19macramphidaChitwood,1951♂1400395,67,99,6пап.1520macrobursataKeppner, 19873♂♂1680–220050–535,713,28,6щет. 10–1223–2932–40meridionalisKreis, 1932♂♂1415–174330–435,3–6,98,2–9,67,3–9,8пап.18–2518–23microsetaWieser, 1953♂1850446,116,54,3щет. 1,5–2,020–2233nudaKreis, 19325♂♂1625–193145–545,2–6,07,3–8,013,0–13,5пап.18–2218oncholaimelloidesWieser, Hopper, 1967♂1950896,513,99,1пап.1317orientalis sp.n.Гагарин, наст. статья3♂♂991–112217–204,7–5,115,7–19,92,8–3,0щет. 2,018–2034–36papillatoidesChitwood, 1960♂♂1520–220040–515,5–5,619,1–23,03,4–4,6пап.24–3226–29papillataChitwood, 19511♂1520315,011,06,0пап.1824parasetosaKreis, 19323♂♂1721–174852–555,7–5,911,6–12,08,2–8,3щет. 2,718–2024profundaVitiello, 19702♂♂1311–150147–505,0–5,614,8–15,44,5–5,2щет. 2,0–2,517,5–18,017,5separabilisWieser, 1953♂♂1320–220032–784,8–7,812,9–16,65,0–8,0щет. 7,520–2619–26setosusKreis, 19323♂♂1784–185751–576,0–6,111,9–12,18,0–9,0щет. 5,020–2122stenolaimaFilipjev, 19271♂1870374,512,64,6пап.25–2737timmiГагарин, Нгуен Тхи Тху, 200810♂♂1016–150922–374,1–5,116,0–25,62,0–3,5щет. 2,5–3,021–2824–28viscosaPlat, Warwick, 1983; Bastian, 1865♂♂1700–260052–605,0–6,512,0–16,06,0–7,5щет. 4,022–3027–30weiseriMawson, 19583♂♂1800–290029–464,3–5,213,3–17,74,5щет. 5,028–30420Примечание: n — чисΛо промеренных самцов; пап. — папиΛΛы; щет. — щетинки
description: Published as part of Gagarin, V. G., 2020, Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam, pp. 31 in Amurian Zoological Journal XII (1) on page 31, DOI: 10.33910/2686-9519-2020-12-1-26-42, http://zenodo.org/record/11088743
DOI: 10.5281/zenodo.15143813
title: ТабΛица 2 Основные морфоΛогические признаки самцов ваΛиΑных виΑов роΑа Viscosia, имеющих ΑΛину теΛа в преΑеΛах 1 – 2 мм Table 2 Basic morphological features of males of the genus Viscosia valid species with body length of 1 – 2 mm in Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam

description: ТабΛица 2 Основные морфоΛогические признаки самцов ваΛиΑных виΑов роΑа Viscosia, имеющих ΑΛину теΛа в преΑеΛах 1–2 мм Table 2 Basic morphological features of males of the genus Viscosia valid species with body length of 1–2 mm
ВиΑΑитературный источникnLabcc ʹФорма и ΑΛина внешних губных сенсиΛΛ, мкмΔΛина стомы, мкмΔΛина спикуΛ, мкмbandaensisKreis 19324♂♂1389–171541–484,8–5,112,8–13,86,0–6,5пап.2318–20bayensisKeppner 19876♂♂1630–182041–485,217,35,6щет. 9–1125–2943–50brachylaimoidesChitwood 19373♂♂1700–254027–396,2–7,414,2–25,03,0щет. 2,0??epapillosaПΛатонова 19711♂1705515,320,74,9щет. 6,01629erasmiFurstenberg, Vincx 19891♂1905605,715,95,0щет. 5,02332glabraBastian 1865; Platt, Warwick 1983♂♂1600–210042–705,3–6,37,5–11,010–13пап.23–2527longicaudatoidesНгуен Ву Тхань, Гагарин, 20132♂♂1348–143253–544,8–5,48,3–9,010,1–10,8щет. 2,3–3,017–1818–19macramphidaChitwood,1951♂1400395,67,99,6пап.1520macrobursataKeppner, 19873♂♂1680–220050–535,713,28,6щет. 10–1223–2932–40meridionalisKreis, 1932♂♂1415–174330–435,3–6,98,2–9,67,3–9,8пап.18–2518–23microsetaWieser, 1953♂1850446,116,54,3щет. 1,5–2,020–2233nudaKreis, 19325♂♂1625–193145–545,2–6,07,3–8,013,0–13,5пап.18–2218oncholaimelloidesWieser, Hopper, 1967♂1950896,513,99,1пап.1317orientalis sp.n.Гагарин, наст. статья3♂♂991–112217–204,7–5,115,7–19,92,8–3,0щет. 2,018–2034–36papillatoidesChitwood, 1960♂♂1520–220040–515,5–5,619,1–23,03,4–4,6пап.24–3226–29papillataChitwood, 19511♂1520315,011,06,0пап.1824parasetosaKreis, 19323♂♂1721–174852–555,7–5,911,6–12,08,2–8,3щет. 2,718–2024profundaVitiello, 19702♂♂1311–150147–505,0–5,614,8–15,44,5–5,2щет. 2,0–2,517,5–18,017,5separabilisWieser, 1953♂♂1320–220032–784,8–7,812,9–16,65,0–8,0щет. 7,520–2619–26setosusKreis, 19323♂♂1784–185751–576,0–6,111,9–12,18,0–9,0щет. 5,020–2122stenolaimaFilipjev, 19271♂1870374,512,64,6пап.25–2737timmiГагарин, Нгуен Тхи Тху, 200810♂♂1016–150922–374,1–5,116,0–25,62,0–3,5щет. 2,5–3,021–2824–28viscosaPlat, Warwick, 1983; Bastian, 1865♂♂1700–260052–605,0–6,512,0–16,06,0–7,5щет. 4,022–3027–30weiseriMawson, 19583♂♂1800–290029–464,3–5,213,3–17,74,5щет. 5,028–30420Примечание: n — чисΛо промеренных самцов; пап. — папиΛΛы; щет. — щетинки
description: Published as part of Gagarin, V. G., 2020, Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam, pp. 31 in Amurian Zoological Journal XII (1) on page 31, DOI: 10.33910/2686-9519-2020-12-1-26-42, http://zenodo.org/record/11088743
DOI: 10.5281/zenodo.15143814
title: ТабΛица 3 Морфометрическая характеристика Halalaimus borealis sp. nov. Table 3 Morphometric featuress of Halalaimus borealis sp. nov. in Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam

description: ТабΛица 3 Морфометрическая характеристика Halalaimus borealis sp. nov. Table 3 Morphometric featuress of Halalaimus borealis sp. nov.
ПризнакГоΛотип самецПаратипы 4 самца Αиапазон среΑнее1 самкаΔΛина теΛа, мкм13731249–142913191511a7275–848076b5,75,4–6,15,76,7c5,55,5–6,95,75,7c ʹ18,314,0–18,116,519,4V, %———38,0Ширина, мкм:обΛасти губ5,55,0–6,05,55,5теΛа в его среΑнем отΑеΛе1915–191620теΛа в обΛасти ануса иΛи кΛоаки1412–151414ΔΛина, мкм:гоΛовных щетинок7,06,0–7,07,57,0фовей амфиΑов4744–474548фаринкса241231–238233225хвоста248214–258230264спикуΛ (по Αуге)2020–2121—руΛька98–98—Расстояние, мкм:от переΑнего конца фовей амфиΑов Αо переΑнего конца теΛа9,08,0–9,08,59,0от конца фаринкса Αо кΛоаки884802–938856—от конца фаринкса Αо вуΛьвы———349от вуΛьвы Αо ануса———673
description: Published as part of Gagarin, V. G., 2020, Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam, pp. 35 in Amurian Zoological Journal XII (1) on page 35, DOI: 10.33910/2686-9519-2020-12-1-26-42, http://zenodo.org/record/11088743
DOI: 10.5281/zenodo.15143815
title: ТабΛица 3 Морфометрическая характеристика Halalaimus borealis sp. nov. Table 3 Morphometric featuress of Halalaimus borealis sp. nov. in Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam

description: ТабΛица 3 Морфометрическая характеристика Halalaimus borealis sp. nov. Table 3 Morphometric featuress of Halalaimus borealis sp. nov.
ПризнакГоΛотип самецПаратипы 4 самца Αиапазон среΑнее1 самкаΔΛина теΛа, мкм13731249–142913191511a7275–848076b5,75,4–6,15,76,7c5,55,5–6,95,75,7c ʹ18,314,0–18,116,519,4V, %———38,0Ширина, мкм:обΛасти губ5,55,0–6,05,55,5теΛа в его среΑнем отΑеΛе1915–191620теΛа в обΛасти ануса иΛи кΛоаки1412–151414ΔΛина, мкм:гоΛовных щетинок7,06,0–7,07,57,0фовей амфиΑов4744–474548фаринкса241231–238233225хвоста248214–258230264спикуΛ (по Αуге)2020–2121—руΛька98–98—Расстояние, мкм:от переΑнего конца фовей амфиΑов Αо переΑнего конца теΛа9,08,0–9,08,59,0от конца фаринкса Αо кΛоаки884802–938856—от конца фаринкса Αо вуΛьвы———349от вуΛьвы Αо ануса———673
description: Published as part of Gagarin, V. G., 2020, Sp. Nov. And Sp. Nov. (Nematoda, Enoplida) From The Mouth Of The Cam River In Vietnam, pp. 35 in Amurian Zoological Journal XII (1) on page 35, DOI: 10.33910/2686-9519-2020-12-1-26-42, http://zenodo.org/record/11088743
DOI: 10.5281/zenodo.15143828
title: Table 1 in Enchodelus repis sp. n. and comments on E. laevis Thorne, 1939 and E. microdoroides Baqri & Jairajpuri, 1974 (Nematoda: Dorylaimoidea) from Korea

description: Table 1. Distance between ventromedian supplements in Enchode/us repis sp. n. males.
Number of ventromedianDistance between supplements (~)Cloacal opening ta anteriormostsupplementssupplementC-SISI-S2S2-S)SrS4S4-SSSS-S62 (n. 1) 4 (n. 8) 5 (n. 6) 6 (n. 2)41 48 (45-56) 45 (41 -50) 44,4823 12 (10- 15) 10 (8- 12) 7, Il14 (1 I-20) li (10- 13) 9, 1216 (9-21) 12 (9- 14) Il, 1213 (11 - 15) 12, 1510, 1264 91 (82-98) 91 (80-96) 99, 104
description: Published as part of Brzeski, Michal W., 1992, Enchodelus repis sp. n. and comments on E. laevis Thorne, 1939 and E. microdoroides Baqri & Jairajpuri, 1974 (Nematoda: Dorylaimoidea) from Korea, pp. 320 in Fundam. appl. Nematol. 15 (4) on page 320, DOI: 10.5281/zenodo.8122500
DOI: 10.5281/zenodo.15143829
title: Table 1 in Enchodelus repis sp. n. and comments on E. laevis Thorne, 1939 and E. microdoroides Baqri & Jairajpuri, 1974 (Nematoda: Dorylaimoidea) from Korea

description: Table 1. Distance between ventromedian supplements in Enchode/us repis sp. n. males.
Number of ventromedianDistance between supplements (~)Cloacal opening ta anteriormostsupplementssupplementC-SISI-S2S2-S)SrS4S4-SSSS-S62 (n. 1) 4 (n. 8) 5 (n. 6) 6 (n. 2)41 48 (45-56) 45 (41 -50) 44,4823 12 (10- 15) 10 (8- 12) 7, Il14 (1 I-20) li (10- 13) 9, 1216 (9-21) 12 (9- 14) Il, 1213 (11 - 15) 12, 1510, 1264 91 (82-98) 91 (80-96) 99, 104
description: Published as part of Brzeski, Michal W., 1992, Enchodelus repis sp. n. and comments on E. laevis Thorne, 1939 and E. microdoroides Baqri & Jairajpuri, 1974 (Nematoda: Dorylaimoidea) from Korea, pp. 320 in Fundam. appl. Nematol. 15 (4) on page 320, DOI: 10.5281/zenodo.8122500
DOI: 10.5281/zenodo.15143830
title: Table 1 in How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?

description: Table 1 Kinetic parameters of P. crispum SDH. V max - maximal reaction rate of enzyme reaction, K - Michaelis constant of the specific substrate, K ′ -apparent m m Michaelis constant at a given concentration of the second substrate), KSS - inhibition constant at an excess substrate concentration, KA - dissociation constant of the enzyme – substrate complex, ♣ for technical reasons (absorbance above 3 or excess substrate inhibition), this parameter could not be determined. Kinetic constants were calculated as an average of values from at least 3 experiments performed in doublets, S. D. are shown.
Direction of the reaction catalyzed bySDHSA → DHSDHS → SApH optimum9.5–107–8.5V [μmol.min 1.mg 1] for SA (DHS) max1.340.29V [μmol.min 1.mg 1] for NADP(H) max1.320.28K m for SA (DHS) [mM]0.71 ± 0.13♣K m for NADP(H) [mM]0.47 ± 0.16♣K ′ for SA (with 0.2 mM NADP) [mM] m0.16 ± 0.01–K ′ for DHS (with 0.4 mM NADPH) [mM] m–0.23 ± 0.03K ′ for NADP (with 3 mM SA) [mM] m0.054 ± 0.011–K ′ for NADPH (with 2 mM DHS) [mM] m–0.016 ± 0.03KSS for SA (DHS) [mM]–0.12 ± 0.07KA [mM]0.030 ± 0.0080.25 ± 0.13
description: Published as part of Hýskova, Veronika, Belonozníkov, Katerina, Smeringaiova, Ingrida, Kavan, Daniel, Ingr, Marek & Ryslava, Helena, 2021, How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?, pp. 2 in Phytochemistry (112881) 190 on page 2, DOI: 10.1016/j.phytochem.2021.112881, http://zenodo.org/record/8270251
DOI: 10.5281/zenodo.15143831
title: Table 1 in How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?

description: Table 1 Kinetic parameters of P. crispum SDH. V max - maximal reaction rate of enzyme reaction, K - Michaelis constant of the specific substrate, K ′ -apparent m m Michaelis constant at a given concentration of the second substrate), KSS - inhibition constant at an excess substrate concentration, KA - dissociation constant of the enzyme – substrate complex, ♣ for technical reasons (absorbance above 3 or excess substrate inhibition), this parameter could not be determined. Kinetic constants were calculated as an average of values from at least 3 experiments performed in doublets, S. D. are shown.
Direction of the reaction catalyzed bySDHSA → DHSDHS → SApH optimum9.5–107–8.5V [μmol.min 1.mg 1] for SA (DHS) max1.340.29V [μmol.min 1.mg 1] for NADP(H) max1.320.28K m for SA (DHS) [mM]0.71 ± 0.13♣K m for NADP(H) [mM]0.47 ± 0.16♣K ′ for SA (with 0.2 mM NADP) [mM] m0.16 ± 0.01–K ′ for DHS (with 0.4 mM NADPH) [mM] m–0.23 ± 0.03K ′ for NADP (with 3 mM SA) [mM] m0.054 ± 0.011–K ′ for NADPH (with 2 mM DHS) [mM] m–0.016 ± 0.03KSS for SA (DHS) [mM]–0.12 ± 0.07KA [mM]0.030 ± 0.0080.25 ± 0.13
description: Published as part of Hýskova, Veronika, Belonozníkov, Katerina, Smeringaiova, Ingrida, Kavan, Daniel, Ingr, Marek & Ryslava, Helena, 2021, How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?, pp. 2 in Phytochemistry (112881) 190 on page 2, DOI: 10.1016/j.phytochem.2021.112881, http://zenodo.org/record/8270251
DOI: 10.5281/zenodo.15143834
title: Table 2 in How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?

description: Table 2 Results of the product inhibition study for the determination of the bisubstrate reaction mechanism of P. crispum SDH. Inhibition constants were calculated as an average of values from 2-4 experiments carried out in doublets, S.D. are shown.NC - non-competitive C - competitive, and N.I. - no inhibition. Patterns and values in brackets mean that the reaction is saturated with the 2 nd substrate.
Product inhibitorProduct inhibitorQ (NADP)P (SA)A (NADPH)B (DHS)Varied substrateVaried substratei.e.1/NADPH1/DHS1/NADPH1/DHS1/NADP1/SA1/NADP1/SA2nd substratesubsaturatedsubsaturated (saturated)PatternCNCCNCC (C)NC (N.I.)NC (N.I.)C (C)Ki [mM]0.22 ± 0.020.4 ± 0.10.7 ± 0.10.7 ± 0.30.12 ± 0.07 (0.49 ± 0.03)0.36±1.4 ± 0.20.13 ± 0.03 (0.33 ± 0.03)0.08Mechanismsteady state sequential ordersteady state sequential order
description: Published as part of Hýskova, Veronika, Belonozníkov, Katerina, Smeringaiova, Ingrida, Kavan, Daniel, Ingr, Marek & Ryslava, Helena, 2021, How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?, pp. 3 in Phytochemistry (112881) 190 on page 3, DOI: 10.1016/j.phytochem.2021.112881, http://zenodo.org/record/8270251
DOI: 10.5281/zenodo.15143835
title: Table 2 in How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?

description: Table 2 Results of the product inhibition study for the determination of the bisubstrate reaction mechanism of P. crispum SDH. Inhibition constants were calculated as an average of values from 2-4 experiments carried out in doublets, S.D. are shown.NC - non-competitive C - competitive, and N.I. - no inhibition. Patterns and values in brackets mean that the reaction is saturated with the 2 nd substrate.
Product inhibitorProduct inhibitorQ (NADP)P (SA)A (NADPH)B (DHS)Varied substrateVaried substratei.e.1/NADPH1/DHS1/NADPH1/DHS1/NADP1/SA1/NADP1/SA2nd substratesubsaturatedsubsaturated (saturated)PatternCNCCNCC (C)NC (N.I.)NC (N.I.)C (C)Ki [mM]0.22 ± 0.020.4 ± 0.10.7 ± 0.10.7 ± 0.30.12 ± 0.07 (0.49 ± 0.03)0.36±1.4 ± 0.20.13 ± 0.03 (0.33 ± 0.03)0.08Mechanismsteady state sequential ordersteady state sequential order
description: Published as part of Hýskova, Veronika, Belonozníkov, Katerina, Smeringaiova, Ingrida, Kavan, Daniel, Ingr, Marek & Ryslava, Helena, 2021, How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?, pp. 3 in Phytochemistry (112881) 190 on page 3, DOI: 10.1016/j.phytochem.2021.112881, http://zenodo.org/record/8270251
DOI: 10.5281/zenodo.15143836
title: Table 3 in How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?

description: Table 3 Identification of SDH products by LC-MS analysis. List of products detected after 30 min incubation with (and without) SDH enzyme at 20 ◦ C. DHS, 3-dehydroshikimate; DHQ, 3-dehydroquinate; PCA, protocatechuic acid; SA, shikimic acid; QA, quinate. *the incubation time was 60 min at 20 ◦ C.SDH from P. crispum root (10 μl with specific activity 470 nmol.min -1.mg -1) was incubated in the reaction mixture (280 μl) containing 2 mM substrate (SA, DHS, DHQ, QA, GA, or PCA) and 0.14 mM cofactor (NADP, NADPH, NAD, NADH) in 100 mM Tris-HCl pH 8 (physiological direction) or 100 mM AMP-NaOH pH 9 at 20 ◦ C for 30 min. The reaction was initiated by adding SDH and stopped by adding 10 μl of 99 % (v/v) formic acid. Blank experiments were prepared similarly, albeit adding formic acid before the enzyme. Each determination was done at least 3-times, the average values and S. D. are shown.
reactionproducts [nmol]SA+ NADPDHS: 5.7 ± 0.7PCA: 0.1 ± 0.0*DHS+ NADPHSA: 4.9 ± 0.2GA: 0.5 ± 0.0PCA: 0.7 ± 0.1DHQ: 1.1 ± 0.0DHS+ NADPGA: 0.1 ± 0.0PCA: 0.3 ± 0.2DHS+ NADPGA: 2.1 ± 0.4– without SDHPCA: 2.7 ± 0.6DHQ+ NADPDHS: 17.4 ± 1.4PCA: 4.9 ± 0.4*DHQ+ NADPHDHS: 3.4 ± 0.8SA: 1.4 ± 0.2GA: 1.1 ± 0.5PCA: 1.3 ± 0.2DHQ+ NADHNo products detected.QA+ NADNo products detected.PCA+ NADPHNo products detected.
description: Published as part of Hýskova, Veronika, Belonozníkov, Katerina, Smeringaiova, Ingrida, Kavan, Daniel, Ingr, Marek & Ryslava, Helena, 2021, How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?, pp. 6 in Phytochemistry (112881) 190 on page 6, DOI: 10.1016/j.phytochem.2021.112881, http://zenodo.org/record/8270251