cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/pjez3-qpn07
title: Vibrio cholerae SAD2112

description: SAD2112 (for T2SS only) = rpoS*, lacZ::lacIq, comEA-mCherry, ΔluxO, Ptac-tfoX, Ptac-recA, ∆TCP::ZeoR, ∆MSHA::CarbR, ∆CTX::KanR, ∆pilMNOPQ::TmR (aka ∆competence pilus) Ankur: "For the taxonomy ID - unfortunately the strain we sent does not have a taxonomy ID. So, you can use the generic one for V. cholerae which is "666". The strain we are working with is related to N16961, but is certainly not the same strain, so not sure if you would want to indicate that somehow? Just for reference - the strain we sent is E7946 (although it is obviously heavily modified with a number of mutations). "
description: <strong>Tilt Series Date:</strong> 2018-07-31</p>
<strong>Data Taken By:</strong> Sara Weaver</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> SAD2112</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ankur B Dalia ankdalia@indiana.edu, PhD, Assistant Professor, Indiana University. Cells grown, data collected by Sara Weaver</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streaked stock on a plate. Grew up a single colony in 3 mL LB with shaking at 37˚C. The next day, subcultured 50uL of this overnight culture into 3mL of fresh LB+100uM IPTG + 20mM MgCl2 + 10mM CaCl2 and grow at 30˚C shaking for ~4 hours.  Note that the induction wasn't necessary for this delta T4 competence pili strain</p>
<strong>Sample Preparation:</strong> On 2018/07/12 spun gold (100 µL 10 nm gold + 25 µL 5% BSA) at 15,000rpm for 20 minutes, removed supernatant. Resuspended gold beads in 20 µL liquid culture. Vitrobot RT and 95% humidity, 3µL of gold/sample slurry on carbon/copper Quantifoil Finder grids R2/2 (EmiTech glow discharge 50 mA 120 s) Blot force +6, blot time 3 s, no waiting time, no drain time. Plunged into ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2018-07-31-21/rawdata/</p>2112I_tomogram20.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2018-07-31-21/rawdata/2112I_tomogram20.mrc"
        > <i class="download icon"></i> Download </a></p>

        2112I_tomogram20_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2018-07-31-21/3dimage_93872/2112I_tomogram20_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r3vas-er263
title: Vibrio cholerae SAD2090

description: Vibrio, T4P, competence pilus
description: <strong>Tilt Series Date:</strong> 2020-09-09</p>
<strong>Data Taken By:</strong> Stefan Kreida</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> SAD2090</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.7 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ankur B Dalia, ankdalia@indiana.edu, PhD, Assistant Professor, Indiana University. . Cell growth & preparation, data collection and frame alignment by Stefan Kreida.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 100 uL O/N preculture used to inoculate 3 mL LB, 20 mM MgSO4, 10 mM CaCl2, 100 uM IPTG. . Cells were grown for 7 h (30C) on 170 rpm. 100 uL culture was then spun down at 5,000 x g (5') . and resuspended in 100 uL aquarium salt (Instant Ocean), 7 g/L. </p>
<strong>Sample Preparation:</strong> BSA/10 nm gold suspension: 1 mL 10 nm gold suspension mixed with 0.25 mL 5% BSA, spun . at 14,000 rpm (10'). Supernatant removed so that 35 uL remained, which was used to . resuspend the gold particles. 30 uL cells mixed with 5 uL gold suspension. 3 uL sample . applied in Vitrobot at 100% humidity. Grid: Quantifoil R2/2, Cu NH2, LotID 186865.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/skv2020-09-09-12/rawdata/</p>G12_SAD2090_TS20_ali.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/skv2020-09-09-12/rawdata/G12_SAD2090_TS20_ali.mrc"
        > <i class="download icon"></i> Download </a></p>

        G12_SAD2090_TS20_ali_part56_12.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/skv2020-09-09-12/3dimage_113401/G12_SAD2090_TS20_ali_part56_12.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dcqzk-2n612
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-17_slicer10316.jpg: Bdellovibrio bacteriovorus .  filament between cells ycw2013-09-28-17_slicer10317.jpg: Bdellovibrio bacteriovorus .  nucleoid ycw2013-09-28-17_slicer14221.jpg: Bdellovibrio bacteriovirus .  Pink: membrane blebbing out / bulging .  Green: something at interface between two cells?  unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-17/rawdata/</p>20130928_YWC034_10017.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-17/rawdata/20130928_YWC034_10017.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10017_part131_9.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-17/3dimage_31383/20130928_YWC034_10017_part131_9.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/92bwa-z9621
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-80_slicer14298.jpg: Bdellovibrio bacteriovorus + E coli mini cell .  density at cell interface ycw2013-09-28-80_slicer14299.jpg: Bdellovibrio bacteriovorus + E coli mini cell .  electron-dense cluster
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-80/rawdata/</p>20130928_YWC034_10088.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-80/rawdata/20130928_YWC034_10088.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10088_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-80/3dimage_31513/20130928_YWC034_10088_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/791tb-bh521
title: Bdellovibrio/E. coli minicell

description: Sample box: YWC053Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion: mixing time 36 min
description: <strong>Tilt Series Date:</strong> 2013-12-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 36 min. Pellet of 10 nm gold beads were first resuspent thoroughly and 20 ul cells were then gently mixed in by tapping the tube</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-12-06-49_slicer6790.jpg: nucleoid .  chemoreceptor ycw2013-12-06-49_slicer6791.jpg: pilli ycw2013-12-06-49_slicer6796.jpg: flagellar motor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-49/rawdata/</p>montage_25x250041.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-49/rawdata/montage_25x250041.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_25x250041_part131_32.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-49/3dimage_35786/montage_25x250041_part131_32.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mbhyj-5f863
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-40_slicer10360.jpg: Bdellovibrio bacteriovorus .  Flagellar motor .  nucleoid ycw2013-09-28-40_slicer10361.jpg: Bdellovibrio bacteriovorus .  inclusion body ycw2013-09-28-40_slicer14248.jpg: Bdellovibrio bacteriovorus .  flagellar motor with arrow pointing to c-ring? .  flagellum
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-40/rawdata/</p>20130928_YWC034_10040.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-40/rawdata/20130928_YWC034_10040.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10040_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-40/3dimage_31426/20130928_YWC034_10040_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ra647-5v623
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-56_slicer10379.jpg: Bdellovibrio bacteriovorus .  OMV ycw2013-09-28-56_slicer14268.jpg: Bdellovibrio bacteriovorus .  electron dense object .  dark blob interface between cells ycw2013-09-28-56_slicer14269.jpg: Bdellovibrio bacteriovorus .  flagellar motor .  flagellum .  chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-56/rawdata/</p>20130928_YWC034_10056.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-56/rawdata/20130928_YWC034_10056.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10056_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-56/3dimage_31464/20130928_YWC034_10056_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vgng9-map22
title: Bdellovibrio2492/E.coli minicell

description: Bdellovibrio HI Strain 2492 in mixture with E. coli WM3433 minicellsGrid box YWC107
description: <strong>Tilt Series Date:</strong> 2014-07-18</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio HI 2492 grown from frozen stocks in liquid PY media at 37C for 7 days, subculturing half of culture into equal volume of fresh media every two days. E. coli minicells WM3433 grown O/N to high OD and spun down 7 mins at 6krpm.  Supernatant spun 10 mins at 14krpm to pellet minicells.  Minicells resuspended in 20ul media and combined in 1:1 v/v ratio with Bdellovibrio.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. Manual blotting, 3 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2014-07-18-26_slicer10481.jpg: Bdellovibrio bacteriovorus HI 2492.  This strain forms long filamentous cells with regularly spaced storage granules.
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-26/rawdata/</p>20140718_Janelia_YWC107_10009.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-26/rawdata/20140718_Janelia_YWC107_10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140718_Janelia_YWC107_10009_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-26/3dimage_45130/20140718_Janelia_YWC107_10009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xsnkn-ga020
title: Bdellovibrio/E. coli minicell

description: Sample box: YWC053Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion: mixing time 36 min
description: <strong>Tilt Series Date:</strong> 2013-12-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 36 min. Pellet of 10 nm gold beads were first resuspent thoroughly and 20 ul cells were then gently mixed in by tapping the tube</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-12-06-3_slicer4210.jpg: Nulceoid .  Granule
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-3/rawdata/</p>montage_25x250045.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-3/rawdata/montage_25x250045.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_25x250045_part131_2.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-3/3dimage_35704/montage_25x250045_part131_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.54yvat
title: Occurrence Download

description: A dataset containing 38549 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Sparganium natans"
 ]
}

 The dataset includes 38549 records from 423 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006438-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.