cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ff31f-cq228
title: Legionella_JV9246

description: Legionella_JV9246: DotF-sfGFP fusion to the wild-type gene on the chromosome (thyA) and ΔdotO.
description: <strong>Tilt Series Date:</strong> 2019-05-28</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-68/rawdata/</p>JV9246_area1_tomo0079.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-68/rawdata/JV9246_area1_tomo0079.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9246_area1_tomo0079_part41_1.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-68/3dimage_103525/JV9246_area1_tomo0079_part41_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hny90-8a998
title: V.cholerae

description: This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.
description: <strong>Tilt Series Date:</strong> 2017-01-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Yi-wei</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: -60, 60.         dosage: 160e/A2. defocus: -6um. magnification: 27500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-27/3dimage_80126/</p>atlas50005_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-27/3dimage_80126/atlas50005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas50005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-27/rawdata/atlas50005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p3p4r-08k88
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2015-10-19</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> AZ21, ΔsdpABC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Zielinska, A., Billini, M., Moll, A., Kremer, K., Briegel, A., Izquierdo Martinez, A., Jensen, G. J. and Thanbichler, M. (2017). Lytm factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106, 419-438.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-19-31/3dimage_70548/</p>AZ21Set0007_part121_10.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-19-31/3dimage_70548/AZ21Set0007_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>

        AZ21Set0007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-19-31/rawdata/AZ21Set0007.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/czmxs-2x503
title: T5_504Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 504 has been mutated to Cys residue. Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-02-17</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.See pages 104-105</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-17-29/rawdata/</p>sq90021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-17-29/rawdata/sq90021.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq90021.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-17-29/3dimage_82494/sq90021.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kyq50-dt282
title: Myxococcus xanthus SA6892

description: Strain SA6892 (minor pilin cluster 2+3 deletion, Δmxan1021-1017; Δmxan1369-1365).Grid box YWC222
description: <strong>Tilt Series Date:</strong> 2016-08-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-27/rawdata/</p>20160822_YWC222_10028.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-27/rawdata/20160822_YWC222_10028.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160822_YWC222_10028_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-27/3dimage_78310/20160822_YWC222_10028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dn8xj-9bn55
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2015-10-01</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> AZ145, ΔldpF Pvan-dipM gentR</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Zielinska, A., Billini, M., Moll, A., Kremer, K., Briegel, A., Izquierdo Martinez, A., Jensen, G. J. and Thanbichler, M. (2017). Lytm factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106, 419-438.grown with 0.5 mM Vanillat</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-51/3dimage_69832/</p>CaulobacterAZ145firstsetB0001_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-51/3dimage_69832/CaulobacterAZ145firstsetB0001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        CaulobacterAZ145firstsetB0001.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-51/rawdata/CaulobacterAZ145firstsetB0001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xg7fe-8w768
title: T5_1550Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1550 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-03-10</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. The humidifier was not working. I wouldn't really trust the conditions. See pages 104-105</p>
<strong>Sample Preparation:</strong> The sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-7/rawdata/</p>sq7_T5_1693cysAulabelled0006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-7/rawdata/sq7_T5_1693cysAulabelled0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq7_T5_1693cysAulabelled0006.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-7/3dimage_83112/sq7_T5_1693cysAulabelled0006.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ae0md-xw922
title: Myxococcus xanthus delta-pilT

description: Grid box YWC129
description: <strong>Tilt Series Date:</strong> 2015-04-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-72/rawdata/</p>20150428_YWC129_20074.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-72/rawdata/20150428_YWC129_20074.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150428_YWC129_20074_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-72/3dimage_55119/20150428_YWC129_20074_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gvk3x-px991
title: Saccharomyces cerevisiae - JAWY1

description: Raw data files of Saccharomyces cerevisiae - JAWY1
description: <strong>Tilt Series Date:</strong> 2016-12-12</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: -100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. Heat shocked @38 deg C for 1hr50min and recovered @28deg C for 20 min (except sm2016-12-12-3 alias TS16 which was heat shocked for 1.5 hr and recovered for 40 min)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-12-12-16/rawdata/</p>TS15.mrc,  Tilt Series (Pixel Size 1.5 nm), 433.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-12-12-16/rawdata/TS15.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS15_4bin_NS_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 397.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-12-12-16/3dimage_81141/TS15_4bin_NS_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x953z-0ha55
title: V.cholerae

description: This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.
description: <strong>Tilt Series Date:</strong> 2017-01-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Yi-wei</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: -60, 60.         dosage: 160e/A2. defocus: -6um. magnification: 27500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-92/3dimage_80469/</p>sq90011_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-92/3dimage_80469/sq90011_full.rec"
        > <i class="download icon"></i> Download </a></p>

        sq90011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-92/rawdata/sq90011.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/092q1-aay69
title: Hippocampal Neurons (cultured)

description: Raw data files of Hippocampal Neurons (cultured)
description: <strong>Tilt Series Date:</strong> 2015-02-15</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 12 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-15-19/rawdata/</p>021515_hipp_neuron0016.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-15-19/rawdata/021515_hipp_neuron0016.mrc"
        > <i class="download icon"></i> Download </a></p>

        021515_hipp_neuron0016_full.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-15-19/3dimage_51952/021515_hipp_neuron0016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jy0wz-t2987
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-31/rawdata/</p>20160424_YWC212_40004.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
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        20160424_YWC212_40004_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
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