DOI: 10.5061/dryad.81q1v
title: Data from: Protection against a fungal pathogen conferred by the aphid facultative endosymbionts Rickettsia and Spiroplasma is expressed in multiple host genotypes and species and is not influenced by co-infection with another symbiont

description: Many insects harbour facultative endosymbiotic bacteria, often more than
 one type at a time. These symbionts can have major effects on their
 hosts' biology, which may be modulated by the presence of other
 symbiont species and by the host's genetic background. We
 investigated these effects by transferring two sets of facultative
 endosymbionts (one Hamiltonella and Rickettsia, the other Hamiltonella and
 Spiroplasma) from naturally double-infected pea aphid hosts into five
 novel host genotypes of two aphid species. The symbionts were transferred
 either together or separately. We then measured aphid fecundity and
 susceptibility to an entomopathogenic fungus. The pathogen-protective
 phenotype conferred by the symbionts Rickettsia and Spiroplasma varied
 among host genotypes, but was not influenced by co-infection with
 Hamiltonella. Fecundity varied across single and double infections and
 between symbiont types, aphid genotypes and species. Some host genotypes
 benefit from harbouring more than one symbiont type.
description: Fecundity
 dataSpreadsheet
 "Fecundity" contains the results of the fecundity assay. The
 aphids used in this experiment either contained no known secondary
 symbionts or were artificially infected with different complements of
 symbiont species. We counted the number of offspring produced by single
 wingless aphids, representing one of five genotypes and hosting one of
 seven symbiont complements, during three (grain aphids) or four (pea
 aphids) consecutive three-day intervals. In the spreadsheet, rows contain
 offspring counts for individual aphids. Columns are as follows:
 &gt;&gt;&gt;Species - Aphid species, two levels (A. pisum, S. avenae).
 &gt;&gt;&gt;Gen - Aphid genotype, five levels (145, J68, J102, Co23,
 Co26). &gt;&gt;&gt;Sym - Symbiont infection status. Ha / Ri / Sp represent
 Hamiltonella defensa, Rickettsia sp. or Spiroplasma sp., and the value
 after underscore represents the genotype of original pea aphid host.
 &gt;&gt;&gt;Block - The ID number of experimental block. In all cases,
 lines of a given genotype were tested at the same time (and so have the
 same block ID number), separately from lines of other genotypes. The
 fecundity assay for genotype 145 was conducted in two blocks.
 &gt;&gt;&gt;D10 - number of offspring produced by single wingless aphids
 before its 10th day of life. &gt;&gt;&gt;D13, D16, D19 - numbers of
 offspring produced by single aphids during consecutive three-day
 intervals. &gt;&gt;&gt;Fec_total - total number of offspring produced
 during 16 days (S. avenae) or 19 days (A. pisum). Missing data are
 indicated by “NA”.Lukasik et al - fecundity
 data.xlsxPathogen susceptibility
 dataSpreadsheet
 "Susceptibility" contains the results of the pathogen
 susceptibility assay. The aphids used in this experiment either contained
 no known secondary symbionts or were artificially infected with different
 complements of symbiont species. Groups of 20 aphids, representing one of
 five genotypes and hosting one of seven symbiont complements, were exposed
 to spores of the pathogenic fungus Pandora neoaphidis. After six days all
 aphids were scored as a) sporulating; b) dead but not sporulating; or c)
 alive. In the spreadsheet, each row represents a replicate with 20 aphids.
 Columns are as follows: &gt;&gt;&gt;Species - Aphid species, two levels
 (A. pisum, S. avenae). &gt;&gt;&gt;Gen - Aphid genotype, five levels (145,
 J68, J102, Co23, Co26) &gt;&gt;&gt;Sym - Symbiont infection status. Ha /
 Ri / Sp represent Hamiltonella defensa, Rickettsia sp. or Spiroplasma sp.,
 and the value after underscore represents the genotype of original pea
 aphid host. &gt;&gt;&gt;Fung_D6 - The number of aphids in a replicate
 which were classified as sporulating six days after the exposure.
 &gt;&gt;&gt;Dead_D6 - The number of aphids in a replicate which were
 classified as dead but not sporulating. &gt;&gt;&gt;Alive_D6 - The number
 of aphids in a replicate which were alive six days after the exposure.
 &gt;&gt;&gt;Total - The total number of aphids in the three previous
 categories. Note that in some cases the total is different than 20 –
 occasionally not all aphids were found six days after the exposure, or the
 number of the exposed aphids in a replicate may have been miscalculated by
 1 or 2.Lukasik et al - pathogen
 susceptibility data.xlsx