DOI: 10.5061/dryad.16737
title: Data from: Three new species in the harvestmen genus Acuclavella (Opiliones, Dyspnoi, Ischyropsalidoidea), including description of male Acuclavella quattuor Shear 1986.

description: In Shear’s 1986 cladistic analysis of the Ischyropsalidoidea, he described
 the new genus Acuclavella including four new species from the Pacific
 Northwest states of Washington and Idaho. Several of these species
 descriptions were based on very limited sample sizes. Our recent field
 work has increased by more than an order of magnitude both the number of
 specimens and known localities for Acuclavella. We use this new material
 to interpret species limits in Acuclavella using morphometric analyses and
 DNA sequence data from four gene regions. We sequence for the first time
 the protein-coding homolog of the WNT2 gene for phylogenetic
 reconstruction in Opiliones. Our multi-locus phylogeny corroborates a
 sister relationship between Acuclavella and Ceratolasma, as hypothesized
 using morphology by Shear (1986). Within Acuclavella, morphometric
 clusters and reciprocal allelic monophyly allows recognition of three
 additional species: Acuclavella leonardi sp. n., A. sheari sp. n., and A.
 makah sp. n. This work also describes the previously unknown male of
 Acuclavella quattuor, from specimens collected at the type locality. Our
 research identifies a number of novel morphologies for Acuclavella,
 including females with four pairs of spines, individuals with three pairs
 of spines on scute areas I-III, and a population with two pairs of spines
 disjunct from A. quattuor, which was diagnosed with this spination
 character. We were unable to assign these populations to existing species,
 and conservatively do not yet recognize them as new. Intrageneric
 morphometrics and phylogenetic inference in Acuclavella were often
 concordant. However, we demonstrate that species delimitation signal would
 not be detected if only a single line of evidence were utilized.
description: Acuclavella_28S_Richart&Hedin28S alignment. 7 Dyspnoi outgroup, 14 Acuclavella ingroup sequences.Acuclavella_COI_Richart&Hedin7 Dyspnoi outgroup, 18 Acuclavella ingroup sequences.Acuclavella_EF1a_Richart&Hedin30 Acuclavella ingroup sequences with intron.Acuclavella_Wnt2_Richart&Hedin5 Dyspnoi outgroup, 11 Acuclavella ingroup sequences.EF1a_Richart&Hedin_infile.nex.conEF-1a consensus tree from Bayesian analysis. 7 Dyspnoi outgroup, 30 Acuclavella ingroup sequences.28S_Richart&Hedin_infile.nex.con28S consensus tree from Bayesian analysis. 7 Dyspnoi outgroup, 14 Acuclavella ingroup sequences.COI_Richart&Hedin_infile.nex.conCOI consensus tree from Bayesian analysis. 7 Dyspnoi outgroup, 30 Acuclavella ingroup sequences.Wnt2_Richart&Hedin_infile.nex.conWNT2 consensus tree from Bayesian analysis. 5 Dyspnoi outgroup, 11 Acuclavella ingroup sequences.Bayes_Acuclavella_Concat_All_RichartHedin_infile.nex.con28S consensus tree from Bayesian analysis. 7 Dyspnoi outgroup, 28 Acuclavella ingroup sequences.RAxML_28S_Richart&Hedin28S RAxML gene tree in Newick tree format with bootstrap values.RAxML_COI_Richart&HedinCOI RAxML gene tree in Newick tree format with bootstrap values.RAxML_Concat_All_Richart&HedinAll 4 loci concatenated RAxML phylogeny in Newick tree format with bootstrap values.RAxML_EF1a_Richart&HedinEF1a RAxML gene tree in Newick tree format with bootstrap values.RAxML_Wnt2_Richart&HedinWNT2 RAxML gene tree in Newick tree format with bootstrap values.Acuclavella_EF1a_Richart&Hedin_Outgroup_IngroupIntronAlignment of 37 sequences: 7 Dyspnoi outgroup and 30 Acuclavella ingroup. Alignment contains introns for ingroup sequences only.