DOI: 10.15468/s9zx4a
title: Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave

description: This dataset contains the digitized treatments in Plazi based on the original journal article Gutierrez-Patricio, Sara, Gonzalez-Pimentel, Jose L., Miller, Ana Zelia, Hermosin, Bernardo, Saiz-Jimenez, Cesareo, Jurado, Valme (2021): Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave. International Journal of Systematic and Evolutionary Microbiology (004942) 71 (8): 1-10, DOI: 10.1099/ijsem.0.004942, URL: http://dx.doi.org/10.1099/ijsem.0.004942AbstractA novel facultatively anaerobic, non-motile, Gram-stain-negative, non-endospore-forming alphaproteobacterium, strain 1011 MAR 3 C25 T, was isolated from a white biofilm colonizing the walls of the Andalusian show cave Gruta de las Maravillas (Huelva, Spain). Strain 1011 MAR 3 C25 T grew at 8 –42 °C (optimum, 20–30°C), at pH 5.0–9.0 (optimum, pH 5.0–6.0) and in the presence of 0–12% (w/v) NaCl (optimum 3–5%). Cells were catalase- and oxidase-positive. The strain grew heterotrophically with various carbon sources and chemoautotrophically with thiosulfate under aerobic conditions. Results of phylogenetic analysis showed that strain 1011 MAR 3 C25 T was related to Paracoccus saliphilus DSM 18447 T and Paracoccus alkanivorans LMG 30882 T (97.90% and 97.32% 16S rRNA sequence identity values, respectively). The major respiratory quinone was ubiquinone Q-10 and the predominant fatty acid was C 18:1 ω 7 c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified polar lipid. The DNA G+C content was 60.3 mol%. Based on a polyphasic taxonomic study it is proposed that strain 1011 MAR 3 C25 T (= CECT 9092 T = LMG 29414 T) represents a novel species of the genus Paracoccus, for which the name Paracoccus onubensis sp. nov. is proposed.The genus Paracoccus belongs to the class Alphaproteobacteria and family Rhodobacteraceae. It was described by Davis et al. [1] with Paracoccus denitrificans as the type species. Members of this genus are Gram-stain-negative, non-spore-forming, usually non-motile, and catalase- and oxidase-positive. The major cellular fatty acid is C 18:1 ω7 c. The G+C content of the DNA is 64–70% mol. Currently the genus Paracoccus includes 74 validly named species and 15 non-validly named species (https://lpsn.dsmz.de/genus/ paracoccus). The general model of metabolism is chemoor - ganotrophic. Some species are able to grow as facultative chemolithoautotrophs using reduced sulphur compounds as substrates and others can live as facultative methylotrophs using methanol as a substrate [2]. Paracocci are ubiquitous and growth occurs in water [3] and soil [4 – 6], among other environments [7]. Paracoccus cavernae was isolated from a cave air [8]. In this study, a bacterial strain, 1011 MAR 3 C25 T, was isolated from white biofilms coating the walls of a cave and characterized using a polyphasic approach to determine its taxonomic position. Strain 1011 MAR 3 C25 T is proposed to represent a novel species within the genus Paracoccus named Paracoccus onubensis sp. nov.