cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.29999641.v1
title: Structural Variations and Phylogenetic Implications of Mitochondrial Genomes in Oaks

description: Despite their pivotal role in eukaryotic evolution, plant mitochondrial genomes remain enigmatic owing to their structural plasticity and limited exploration in nonmodel woody species—a critical knowledge gap that hinders a comprehensive understanding of plant diversification mechanisms. As a keystone genus in Northern Hemisphere ecosystems, Quercus species exhibit exceptional ecological adaptability and phylogenetic diversity, serving as a model clade for studying plant diversification and adaptation. Here, we assembled and annotated complete mitochondrial genomes from 15 phylogenetically representative Quercus species via a hybrid sequencing approach combining next-generation and long-read sequencing technologies. Our analyses revealed six distinct mitochondrial genome architectures with sizes ranging from 339 kb to 622 kb. These genomes encode 34-41 genes, 20-28 tRNAs, and 2-5 rRNAs, demonstrating remarkable intragenus variation in organellar gene inventories. Notably, we identified extensive horizontal gene transfer events between the mitochondrial and chloroplast genomes, involving 11-24 migrated gene fragments with transferred sequences spanning 5,265 bp to 12,723 bp. Comparative repeat analysis demonstrated that dispersed repeats predominantly drive mitochondrial genome expansion, with total repeat length showing a significant positive correlation with genome size (R2=0.64, P&lt;0.001). Phylogenomic reconstruction based on 39 concatenated mitochondrial genes resolved deep evolutionary relationships within Quercus, revealing substantial topological discordance with nuclear genome-derived phylogenies and suggesting distinct cytoplasmic vs. nuclear inheritance histories. This study provides crucial genomic resources and evolutionary insights that refine our understanding of organellar genome dynamics in oaks while establishing a framework for resolving cytonuclear discordance in plant phylogenomics.
DOI: 10.22002/7pcx2-1a179
title: O. tauri @ G1

description: O. tauri cell synchronization .
description: <strong>Tilt Series Date:</strong> 2008-03-21</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -14.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Block at G1/S 12h w/Aphidicolin, block 3h w/Propyzamide, transfer</p>
<strong>Sample Preparation:</strong> C-flat LF 4/2 thick carbon, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2008-03-21-7/rawdata/</p>RCC745_G0_01.mrc,  Tilt Series (Pixel Size 1.26 nm), 956.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2008-03-21-7/rawdata/RCC745_G0_01.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G0_01.rec,  Reconstruction (Pixel Size 2.52 nm), 504.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2008-03-21-7/3dimage_3302/RCC745_G0_01.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z1ats-f4993
title: CenK Sacculus

description: C. crescentus CenK sacculus .
description: <strong>Tilt Series Date:</strong> 2007-05-05</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 2/2, BSA treated 10nm gold, 100% humidity, -4mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-3/rawdata/</p>CenK_04.mrc,  Tilt Series (Pixel Size 0.96 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-3/rawdata/CenK_04.mrc"
        > <i class="download icon"></i> Download </a></p>

        CenK_04.rec,  Reconstruction (Pixel Size 0.96 nm), 394.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-3/3dimage_3012/CenK_04.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a1w6e-4kp62
title: CCcenK_glu

description: C. crescentus CenK, glucose depletion .
description: <strong>Tilt Series Date:</strong> 2006-09-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.7°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 36 hours in PYE + 0.1% glucose</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-09-23-2/rawdata/</p>CCcenK_glu13.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-09-23-2/rawdata/CCcenK_glu13.mrc"
        > <i class="download icon"></i> Download </a></p>

        CCcenK_glu13.rec,  Reconstruction (Pixel Size 1.34 nm), 519.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-09-23-2/3dimage_2994/CCcenK_glu13.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.yvreqm
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Fraxinus hupehensis"
 ]
}

 The dataset includes 6 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002669-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ky5vf-xte88
title: CCcenR_glu

description: C. crescentus CenR, glucose depletion .
description: <strong>Tilt Series Date:</strong> 2006-07-06</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 56.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, BSA treated 10nm gold, 100% humidity, -2mm offset,</p>
  lga2006-07-06-8_slicer9408.jpg: Caulobacter crescentus .  periplasmic vesicle lga2006-07-06-8_slicer9409.jpg: caulobacter crescentus .  periplasmic vesicles lga2006-07-06-8_slicer9410.jpg: Caulobacter crescentus .  extracellular vesicles .  OMV
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-8/rawdata/</p>CCcenR_glu03.mrc,  Tilt Series (Pixel Size 1.2 nm), 956.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-8/rawdata/CCcenR_glu03.mrc"
        > <i class="download icon"></i> Download </a></p>

        CCcenR_glu03.rec,  Reconstruction (Pixel Size 2.4 nm), 314.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2006-07-06-8/3dimage_2958/CCcenR_glu03.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ad65e-tm695
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-64/rawdata/</p>20131221_YWC063_20021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-64/rawdata/20131221_YWC063_20021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_20021_part126_1.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-64/3dimage_36468/20131221_YWC063_20021_part126_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8fwwv-7gz49
title: CenK Sacculus

description: C. crescentus CenK sacculus .
description: <strong>Tilt Series Date:</strong> 2007-05-05</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 2/2, BSA treated 10nm gold, 100% humidity, -4mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-8/rawdata/</p>CenK_01.mrc,  Tilt Series (Pixel Size 0.96 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-8/rawdata/CenK_01.mrc"
        > <i class="download icon"></i> Download </a></p>

        CenK_01.rec,  Reconstruction (Pixel Size 0.96 nm), 556.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-05-8/3dimage_3026/CenK_01.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7bskp-r3v53
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C123
description: <strong>Tilt Series Date:</strong> 2012-03-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-1/3dimage_69094/</p>12mar28_pnlDN_C123_1_AT4BT1_0001_part82_4.rec,  Reconstruction (Pixel Size 2.0 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-1/3dimage_69094/12mar28_pnlDN_C123_1_AT4BT1_0001_part82_4.rec"
        > <i class="download icon"></i> Download </a></p>

        12mar28_pnlDN_C123_1_AT4BT1_0001.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/czt82-s6776
title: CenR Sacculus

description: C. crescentus CenR sacculus .
description: <strong>Tilt Series Date:</strong> 2007-05-18</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Depleted 40 hours in PYE + 0.1% glucose; Purified by hot SDS</p>
<strong>Sample Preparation:</strong> C-flat 2/2, BSA treated 10nm gold, 100% humidity, -4mm offset,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-18-1/rawdata/</p>CenR_08.mrc,  Tilt Series (Pixel Size 0.96 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-18-1/rawdata/CenR_08.mrc"
        > <i class="download icon"></i> Download </a></p>

        CenR_08.rec,  Reconstruction (Pixel Size 0.96 nm), 733.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2007-05-18-1/3dimage_3022/CenR_08.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.4wxxr3
title: Occurrence Download

description: A dataset containing 33359 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Alisma lanceolatum"
 ]
}

 The dataset includes 33359 records from 750 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002665-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/29890-c8v52
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-11-16</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-16/rawdata/</p>RCC745_MGtovis_414.mrc,  Tilt Series (Pixel Size 1.26 nm), 746.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-16/rawdata/RCC745_MGtovis_414.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_414.rec,  Reconstruction (Pixel Size 1.26 nm), 558.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-16/3dimage_11797/RCC745_MGtovis_414.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.zdcjhf
title: Occurrence Download

description: A dataset containing 854 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea douglasii",
 "Country is United Kingdom of Great Britain and Northern Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 854 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002670-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/8330z-06t36
title: A22-treated wild type

description: Raw data files of A22-treated wild type
description: <strong>Tilt Series Date:</strong> 2009-07-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR Peach</p>
<strong>Collaborators and Roles:</strong> Cell culture and treatment by Zhuo Li. Data collected by Zhuo Li. Processed by Zhuo Li. </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Exponentially growing NA1000 cells were treated with 10 ug/ml A22 for 2 hours before freezing. </p>
<strong>Sample Preparation:</strong> 10 nm gold. </p>
  zla2009-07-21-57_slicer19405.jpg: pilus Lujia
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-07-21-57/rawdata/</p>09jul21a_A22wt_057.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-07-21-57/rawdata/09jul21a_A22wt_057.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jul21a_A22wt_057_full.rec,  Reconstruction (Pixel Size 2.4 nm), 367.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-07-21-57/3dimage_5938/09jul21a_A22wt_057_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/trxzf-g9r37
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-137/rawdata/</p>RCC745_RELtovis_149.mrc,  Tilt Series (Pixel Size 1.26 nm), 562.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-137/rawdata/RCC745_RELtovis_149.mrc"
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        RCC745_RELtovis_149.rec,  Reconstruction (Pixel Size 1.26 nm), 250.3 MB  
        
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