cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14326882.v1
title: Absorption kinetics and nitrogen fractions in rice as an expression of the OsDof26 transcription factor

description: Dof transcription factors can act as transcriptional activators or repressors in several biological processes unique to plants, however many of their physiological functions have not been explained. In this work, rice plants (Oryza sativa L.), expressing the transcription factor OsDof26, showed changes in uptake kinetics and nitrogen (N) metabolism when subjected to treatments of 0.2 and 2.0 mM ammonium (NH4+) or nitrate (NO3-) in a nutrient solution. The experimental design was completely randomised, with four replications in a 2 × 2 × 2 factorial, two plants (nontransformed rice plants and the L24 strain of rice, expressing OsDof26; two sources of N (NO3- and NH4+); and two dosages (0.2 and 2.0 mM). At 17 days after germination (DAG), the treatments were applied and nutrient solution was then collected at intervals of 30 minutes for 48 hours, in order to estimate the kinetic uptake parameters for N (Vmax, Kmand Cmin). At 19 DAG, the total fresh weight was obtained, with part of the material being stored to determine the soluble nitrogen fractions and soluble sugars. The L24 strain showed less growth of shoots and roots for all the treatments studied compared to the untransformed plant (WT - wild type). The lower influx of NO3-and NH4+ in the L24 resulted in unfavourable kinetic parameters and lower levels of NO3-, NH4+ and amino N, thereby affecting the N metabolism in the transformed plants.
DOI: 10.6084/m9.figshare.14326885
title: Toothpick test: a methodology for the detection of RR soybean plants1

description: Due to the large increase in the area cultivated with genetically modified soybean in Brazil, it has become necessary to determine methods that are fast and efficient for detecting these cultivars. The aim of this work was to test the efficiency of the toothpick method in the detection of RR soybean plants, as well as to distinguish between cultivars, for sensitivity caused by herbicide. Ten transgenic soybean cultivars, resistant to the active ingredient glyphosate, and ten conventional soybean cultivars were used. Toothpicks soaked in glyphosate were applied to all the plants at stage V6 and evaluations were made at 2, 4, 6, 8 and 10 days after application (DAA). The effects of the glyphosate on the cultivars, and the symptoms of phytotoxicity caused in the transgenic plants were evaluated by means of grading scales. The toothpick test is effective in identifying RR soybean cultivars and also in separating them into groups by sensitivity to the symptoms caused by the glyphosate.
DOI: 10.6084/m9.figshare.14326885.v1
title: Toothpick test: a methodology for the detection of RR soybean plants1

description: Due to the large increase in the area cultivated with genetically modified soybean in Brazil, it has become necessary to determine methods that are fast and efficient for detecting these cultivars. The aim of this work was to test the efficiency of the toothpick method in the detection of RR soybean plants, as well as to distinguish between cultivars, for sensitivity caused by herbicide. Ten transgenic soybean cultivars, resistant to the active ingredient glyphosate, and ten conventional soybean cultivars were used. Toothpicks soaked in glyphosate were applied to all the plants at stage V6 and evaluations were made at 2, 4, 6, 8 and 10 days after application (DAA). The effects of the glyphosate on the cultivars, and the symptoms of phytotoxicity caused in the transgenic plants were evaluated by means of grading scales. The toothpick test is effective in identifying RR soybean cultivars and also in separating them into groups by sensitivity to the symptoms caused by the glyphosate.
DOI: 10.6084/m9.figshare.14326887
title: Spatial variability of macronutrients in a crop of conilon coffee in the north of Espírito Santo

description: ABSTRACTThe soils in the state of Espírito Santo, Brazil (ES) display low natural fertility, which often limits the production of the crops due to the deficiency of some elements. However, they have a high potential for agricultural production when the chemical limitations which are present are overcome. The aim of this study was to describe the spatial variability of the macronutrients calcium (Ca), magnesium (Mg), phosphorus (P) and potassium (K) in a crop of conilon coffee (Coffea canephora Pierre) located in the rural area of the city of São Mateus, ES. The experiment was carried out on a crop, planted at a spacing of 1.8 x 1.0 m (5,555 plants ha-1). A rectangular grid of 61.6 x 20.0 m (1,232 m2) was considered, with 60 sampling points, spaced 5.6 m apart between rows and 5.0 m within each crop row. At each point in the sampling grid, soil samples were collected at a depth of 0.00-0.20 m. All the macronutrients under study displayed a strong spatial dependence. The greatest range of spatial dependence was observed for Mg (32.4 m) and the smallest for Ca (8.1 m). Study of the spatial variability of the chemical properties of the soil by geostatistics, using kriging, proved to be an important tool in understanding the spatial distribution of macronutrients in the soil, and may be crucial in aiding the decision making that helps to attend the nutritional requirements of the conilon coffee crop.
DOI: 10.6084/m9.figshare.14326887.v1
title: Spatial variability of macronutrients in a crop of conilon coffee in the north of Espírito Santo

description: ABSTRACTThe soils in the state of Espírito Santo, Brazil (ES) display low natural fertility, which often limits the production of the crops due to the deficiency of some elements. However, they have a high potential for agricultural production when the chemical limitations which are present are overcome. The aim of this study was to describe the spatial variability of the macronutrients calcium (Ca), magnesium (Mg), phosphorus (P) and potassium (K) in a crop of conilon coffee (Coffea canephora Pierre) located in the rural area of the city of São Mateus, ES. The experiment was carried out on a crop, planted at a spacing of 1.8 x 1.0 m (5,555 plants ha-1). A rectangular grid of 61.6 x 20.0 m (1,232 m2) was considered, with 60 sampling points, spaced 5.6 m apart between rows and 5.0 m within each crop row. At each point in the sampling grid, soil samples were collected at a depth of 0.00-0.20 m. All the macronutrients under study displayed a strong spatial dependence. The greatest range of spatial dependence was observed for Mg (32.4 m) and the smallest for Ca (8.1 m). Study of the spatial variability of the chemical properties of the soil by geostatistics, using kriging, proved to be an important tool in understanding the spatial distribution of macronutrients in the soil, and may be crucial in aiding the decision making that helps to attend the nutritional requirements of the conilon coffee crop.
DOI: 10.6084/m9.figshare.14326888.v1
title: Carbon dynamics in no-till soil due to the use of industrial organic waste and mineral fertilizer

description: ABSTRACTThe use of organic waste from industrial processes in agriculture is a strategy not only for improving soil properties but also for promoting the utilization of recycled nutrients by market crops and for reducing environmental impact. The aim of this study was to evaluate the effects of using organic waste from pork and poultry slaughterhouses (OWS) applied alone or in combination with mineral fertilizer (MF) on the dynamics of soil organic matter (SOM) compartments. The experimental design adopted was that of completely randomized blocks with six treatments and three replicates. The treatments consisted of a general control (T1) without the addition of MF and OWS, the application of MF alone at 100% of the recommended fertilizer levels for the crops (T2),the application of OWS alone at a fixed dose of 2 Mg ha-1 (T3), and the following three combinations of MF and OWS: 75% MF + 25% OWS (T4); 50% MF + 50% OWS (T5); and 25% MF + 75% OWS (T6). The application of OWS promoted increase in the labile fractions extracted by potassium permanganate (C-OXP) and hot water (C-HW) compared with using MF alone. Using OWS in the combination of 50% MF + 50% OWS increased the content and stock of total organic carbon (TOC) in the 0-20 cm layer and of particulate organic C (POC) and C-OXP in the 0-5 cm layer.
DOI: 10.6084/m9.figshare.14326888
title: Carbon dynamics in no-till soil due to the use of industrial organic waste and mineral fertilizer

description: ABSTRACTThe use of organic waste from industrial processes in agriculture is a strategy not only for improving soil properties but also for promoting the utilization of recycled nutrients by market crops and for reducing environmental impact. The aim of this study was to evaluate the effects of using organic waste from pork and poultry slaughterhouses (OWS) applied alone or in combination with mineral fertilizer (MF) on the dynamics of soil organic matter (SOM) compartments. The experimental design adopted was that of completely randomized blocks with six treatments and three replicates. The treatments consisted of a general control (T1) without the addition of MF and OWS, the application of MF alone at 100% of the recommended fertilizer levels for the crops (T2),the application of OWS alone at a fixed dose of 2 Mg ha-1 (T3), and the following three combinations of MF and OWS: 75% MF + 25% OWS (T4); 50% MF + 50% OWS (T5); and 25% MF + 75% OWS (T6). The application of OWS promoted increase in the labile fractions extracted by potassium permanganate (C-OXP) and hot water (C-HW) compared with using MF alone. Using OWS in the combination of 50% MF + 50% OWS increased the content and stock of total organic carbon (TOC) in the 0-20 cm layer and of particulate organic C (POC) and C-OXP in the 0-5 cm layer.
DOI: 10.6084/m9.figshare.14326890
title: Production, water consumption and nutrient content of Chinese cabbage grown hydroponically in brackish water

description: ABSTRACTUnderground water reserves in the semi-arid region of Brazil are stored in the crystal formations that in large part have high concentrations of salt. However, the scarcity of this resource makes the use of this water necessary for various activities, including agriculture. The aim of this study was to evaluate the use of brackish water on the electrical conductivity and pH of the nutrient solution, and on the production, water consumption and the uptake of nutrients in Chinese cabbage (Brassica pekinensis L.) under a hydroponic system. The experimental design was completely randomised, with four replications and six levels of water salinity (0.2, 1.2, 2.2, 3.2, 4.2 and 5.2 dS m-1), obtained by adding NaCl to the local water supply, with this water being used to prepare the nutrient solution and to make up the volume lost through evapotranspiration. The results showed that with the exception of the treatment at the lowest salinity, there was a tendency to increased electrical conductivity of the nutrient solution with the increasing salinity of the water; that the pH of the nutrient solution remained within the normal range throughout the cycle; that with the increasing salinity of the solution there was a reduction in all growth and production variables under analysis, in water consumption, and in leaf N, K, Ca and Mg, and an increase in leaf concentrations of Na and Cl. It is possible to use brackish water for the production of Chinese cabbage when grown hydroponically as an alternative for those producers who have an available supply of brackish water and a restricted supply of fresh water, however with a reduction in productivity.
DOI: 10.6084/m9.figshare.14326890.v1
title: Production, water consumption and nutrient content of Chinese cabbage grown hydroponically in brackish water

description: ABSTRACTUnderground water reserves in the semi-arid region of Brazil are stored in the crystal formations that in large part have high concentrations of salt. However, the scarcity of this resource makes the use of this water necessary for various activities, including agriculture. The aim of this study was to evaluate the use of brackish water on the electrical conductivity and pH of the nutrient solution, and on the production, water consumption and the uptake of nutrients in Chinese cabbage (Brassica pekinensis L.) under a hydroponic system. The experimental design was completely randomised, with four replications and six levels of water salinity (0.2, 1.2, 2.2, 3.2, 4.2 and 5.2 dS m-1), obtained by adding NaCl to the local water supply, with this water being used to prepare the nutrient solution and to make up the volume lost through evapotranspiration. The results showed that with the exception of the treatment at the lowest salinity, there was a tendency to increased electrical conductivity of the nutrient solution with the increasing salinity of the water; that the pH of the nutrient solution remained within the normal range throughout the cycle; that with the increasing salinity of the solution there was a reduction in all growth and production variables under analysis, in water consumption, and in leaf N, K, Ca and Mg, and an increase in leaf concentrations of Na and Cl. It is possible to use brackish water for the production of Chinese cabbage when grown hydroponically as an alternative for those producers who have an available supply of brackish water and a restricted supply of fresh water, however with a reduction in productivity.
DOI: 10.6084/m9.figshare.14326893.v1
title: Test of electrical conductivity in evaluating the physiological quality of coriander seeds

description: ABSTRACTThe tests of germination and vigour are essential components of the quality control process of seed producers. Therefore, the aim of this research was to study the methodology of the test of electrical conductivity, verifying its efficiency in identifying different levels of vigour in batches of coriander seed. To do this, four cultivars were used (Português, Super Verdão, Tabocas and Verdão), each represented by four batches of seed, which were initially subjected to evaluations of moisture content, germination, germination first count and seedling emergence. The test of electrical conductivity was carried out on 25 to 50 seeds soaked in 50 and 75 mL of distilled water at 25 °C for 2, 8 and 24 hours. The test of electrical conductivity is efficient to evaluate the physiological potential of coriander seeds when carried out with 50 seeds immersed in 50 mL of distilled water at 25 °C, after soaking for two hours.
DOI: 10.6084/m9.figshare.14326893
title: Test of electrical conductivity in evaluating the physiological quality of coriander seeds

description: ABSTRACTThe tests of germination and vigour are essential components of the quality control process of seed producers. Therefore, the aim of this research was to study the methodology of the test of electrical conductivity, verifying its efficiency in identifying different levels of vigour in batches of coriander seed. To do this, four cultivars were used (Português, Super Verdão, Tabocas and Verdão), each represented by four batches of seed, which were initially subjected to evaluations of moisture content, germination, germination first count and seedling emergence. The test of electrical conductivity was carried out on 25 to 50 seeds soaked in 50 and 75 mL of distilled water at 25 °C for 2, 8 and 24 hours. The test of electrical conductivity is efficient to evaluate the physiological potential of coriander seeds when carried out with 50 seeds immersed in 50 mL of distilled water at 25 °C, after soaking for two hours.
DOI: 10.6084/m9.figshare.14326894.v1
title: Application of molybdenum and a desiccant herbicide to the common bean under direct seeding

description: ABSTRACTApplying micronutrients together with the herbicides used in plant desiccation can ensure an adequate supply of these nutrients and a reduction in production costs under a direct seeding (DSS) system. The aim of this study was to evaluate the effect of dosages of molybdenum (Mo), applied both in a desiccation operation together with glyphosate on the straw of brachiaria (Brachiaria brizantha) and separately via the leaves, on the common bean (cultivar Ouro Vermelho) grown under DSS. A randomised block design was used with four replications in an arrangement of sublots. In the lots, dosages of Mo (0, 0.1, 0.2, 0.4 and 0.8 kg ha-1) were applied together with the desiccant herbicide (glyphosate), and in the sublots, two dosages of Mo (0 and 100 g ha-1) were applied via the leaves. Two experiments were carried out in the town of Coimbra, in the state of Minas Gerais, Brazil, one in 2009 and the other in 2010 (the same treatments having been applied in the experimental area in 2008). Foliar fertilization was carried out with sodium molybdate [(Na2MoO4) (39% Mo)] at stage V4 in the bean plants. The application of Mo, whether mixed with the desiccant or applied via the leaves, had no significant effect on yield components or on productivity. Levels of Mo and N in the grain, and of N in the leaves, increased with the application of Mo, both when mixed with the desiccant and applied via the leaves.
DOI: 10.6084/m9.figshare.14326894
title: Application of molybdenum and a desiccant herbicide to the common bean under direct seeding

description: ABSTRACTApplying micronutrients together with the herbicides used in plant desiccation can ensure an adequate supply of these nutrients and a reduction in production costs under a direct seeding (DSS) system. The aim of this study was to evaluate the effect of dosages of molybdenum (Mo), applied both in a desiccation operation together with glyphosate on the straw of brachiaria (Brachiaria brizantha) and separately via the leaves, on the common bean (cultivar Ouro Vermelho) grown under DSS. A randomised block design was used with four replications in an arrangement of sublots. In the lots, dosages of Mo (0, 0.1, 0.2, 0.4 and 0.8 kg ha-1) were applied together with the desiccant herbicide (glyphosate), and in the sublots, two dosages of Mo (0 and 100 g ha-1) were applied via the leaves. Two experiments were carried out in the town of Coimbra, in the state of Minas Gerais, Brazil, one in 2009 and the other in 2010 (the same treatments having been applied in the experimental area in 2008). Foliar fertilization was carried out with sodium molybdate [(Na2MoO4) (39% Mo)] at stage V4 in the bean plants. The application of Mo, whether mixed with the desiccant or applied via the leaves, had no significant effect on yield components or on productivity. Levels of Mo and N in the grain, and of N in the leaves, increased with the application of Mo, both when mixed with the desiccant and applied via the leaves.
DOI: 10.6084/m9.figshare.14326897.v1
title: Seed quality, chlorophyll content index and leaf nitrogen levels in maize inoculated with Azospirillum brasilense

description: ABSTRACTThe aim of this study was to evaluate differences between maize genotypes in relation to the germination response of the seeds and the growth of seedlings inoculated with Azospirillum brasilense, as well as the effect of inoculation on nitrogen levels and the chlorophyll content index of the leaves. The physiological seeds quality from the single-cross hybrids AG7098 and 2B707, and from the experimental synthetic varieties V2 and V4, inoculated with A. brasilense, was tested for germination, percentage and rate of emergence, and dry matter weight of the shoots and roots. Nitrogen levels and chlorophyll content index were evaluated in leaves of the same four genotypes grown in a greenhouse under different nitrogen supply systems and methods of inoculation with A. brasilense. The genotypes differ with regard to inoculation with A. brasilense. The hybrids were responsive to inoculation with A. brasilense for root dry matter weight. The V2 variety had a lower performance as regards root dry matter weight, with the opposite being seen for the dry matter weight of the shoots. V4 displayed no significant differences when inoculated. The results of the chlorophyll content index were not significant. Each genotype under evaluation displayed a different response for leaf nitrogen levels. It is possible to infer that the hybrids responded better to inoculation with the bacteria, with the greater root development leading to a better utilisation of water and nutrients.
DOI: 10.6084/m9.figshare.14326897
title: Seed quality, chlorophyll content index and leaf nitrogen levels in maize inoculated with Azospirillum brasilense

description: ABSTRACTThe aim of this study was to evaluate differences between maize genotypes in relation to the germination response of the seeds and the growth of seedlings inoculated with Azospirillum brasilense, as well as the effect of inoculation on nitrogen levels and the chlorophyll content index of the leaves. The physiological seeds quality from the single-cross hybrids AG7098 and 2B707, and from the experimental synthetic varieties V2 and V4, inoculated with A. brasilense, was tested for germination, percentage and rate of emergence, and dry matter weight of the shoots and roots. Nitrogen levels and chlorophyll content index were evaluated in leaves of the same four genotypes grown in a greenhouse under different nitrogen supply systems and methods of inoculation with A. brasilense. The genotypes differ with regard to inoculation with A. brasilense. The hybrids were responsive to inoculation with A. brasilense for root dry matter weight. The V2 variety had a lower performance as regards root dry matter weight, with the opposite being seen for the dry matter weight of the shoots. V4 displayed no significant differences when inoculated. The results of the chlorophyll content index were not significant. Each genotype under evaluation displayed a different response for leaf nitrogen levels. It is possible to infer that the hybrids responded better to inoculation with the bacteria, with the greater root development leading to a better utilisation of water and nutrients.
DOI: 10.6084/m9.figshare.14326899.v1
title: Effect of legume hay on the performance of suckling calves

description: ABSTRACTThe aim of this study was to evaluate the performance of suckling calves fed diets with and without legume hay. The experimental period was of 60 days, with solid food being offered ad libitum and available from the 10 th day of the life of the animal, and milk in natura restricted to 4 litres per day, divided into two equal meals. The following diets were evaluated: concentrated feed, concentrated feed and leucaena hay (Leucaena leucocephala Lam. De Wit) and concentrated feed with alfalfa hay (Medicago sativa L.). Fifteen Girolando animals were used, distributed over three treatments in an unbalanced random block design. The parameters under evaluation were: initial body weight (at birth - IBW), final body weight (FBW), average daily gain (ADG), wither height (HW), croup height (HC), thoracic perimeter (PT), dry matter intake (DMI) and feed conversion (FC). No difference was seen in animal performance in relation to the treatments, with averages of 32.9 (±2.9) kg, 67.7 (±1.2) kg, 0.58 (±0.02) kg, 13.1 (±0.5) cm, 13.7 (±0.3) cm, 20.6 (±0.7) cm, 0.46 (±0.1) and 0.79 kg (±0.1) for the parameters IBW, FBW, ADG, HW, HC, PT, DMI and FC respectively. The nutritional requirements of suckling calves are met by supplying milk and concentrated feed; there being no need to include legume hay in the diet.