cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.10365598
title: Dec2023 reference datasets for Mitohelper

description: Reference datasets (Dec2023 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Dec 2023 update: COI dataset was cleaned up in Dec2023 to remove non-COI records.
Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.
I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:

mitofish.all.Dec2023.tsv (835,391 records)
mitofish.12S.Dec2023.tsv (53,390 records)
mitofish.12S.Dec2023_NR.fasta (FASTA file of 12S rRNA gene records)
mitofish.COI.Dec2023.tsv (304,094 records; non-COI records removed in this version)
II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:

12S-seqs-derep-uniq.qza
12S-tax-derep-uniq.qza
12S-16S-18S-seqs.qza
12S-16S-18S-tax.qza
If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.10477520
title: Jan2024 reference datasets for Mitohelper

description: Reference datasets (Jan 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)



Dec 2023 update: COI dataset was cleaned up in Dec2023 to remove non-COI records.


Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Jan2024.tsv (839,731 records)

mitofish.12S.Jan2024.tsv (54,390 records)

mitofish.12S.Jan2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Jan2024.tsv (306,475 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.10655481
title: Feb2024 reference datasets for Mitohelper

description: Reference datasets (Feb 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)



Dec 2023 update: COI dataset was cleaned up in Dec2023 to remove non-COI records.


Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Feb2024.tsv (844,071 records)

mitofish.12S.Feb2024.tsv (55,390 records)

mitofish.12S.Feb2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Feb2024.tsv (308,857 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.11040217
title: Apr2024 reference datasets for Mitohelper

description: Reference datasets (Apr 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Apr2024.tsv (877,543 records)

mitofish.12S.Apr2024.tsv (59,616 records)

mitofish.12S.Apr2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Apr2024.tsv (327,853 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.11494903
title: Jun2024 reference datasets for Mitohelper

description: Reference datasets (Jun 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)



Jun2024: Some duplicate records were found. De-duplicated all records in TSV file.


Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Jun2024.tsv (855,806 records; de-duplicated June2024)

mitofish.12S.Jun2024.tsv (59,809 records; de-duplicated June2024)

mitofish.12S.Jun2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Jun2024.tsv (308,354 records; de-duplicated June2024)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.12773756
title: Jul2024 reference datasets for Mitohelper

description: Reference datasets (Jul 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Jul2024.tsv (864,107 records)

mitofish.12S.Jul2024.tsv (60,281 records)

mitofish.12S.Jul2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Jul2024.tsv (312,741 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.13286432
title: Aug2024 reference datasets for Mitohelper

description: Reference datasets (Aug 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Aug2024.tsv (866,687 records)

mitofish.12S.Aug2024.tsv (60,333 records)

mitofish.12S.Aug2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Aug2024.tsv (314,334 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.13742317
title: Sep2024 reference datasets for Mitohelper

description: Reference datasets (Sep 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)



Sep 2024 Update: Double-checked long (>1,000 bp) 12S rRNA sequences and removed flanking non-12S sequences from some records 


Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Sep2024.tsv (870,372 records)

mitofish.12S.Sep2024.tsv (60,501 records)

mitofish.12S.Sep2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Sep2024.tsv (316,251 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.14104370
title: Nov2024 reference datasets for Mitohelper

description: Reference datasets (Nov 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)



Nov 2024 Update: Removed 12S rRNA sequences that are <50 bp. 


Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Nov2024.tsv (872,797 records)

mitofish.12S.Nov2024.tsv (60,426 records of 12S rRNA gene >50 bp long)

mitofish.12S.Nov2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Nov2024.tsv (317,445 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.14394787
title: Dec2024 reference datasets for Mitohelper

description: Reference datasets (Dec 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Dec2024.tsv (874,975 records)

mitofish.12S.Dec2024.tsv (60,610 records of 12S rRNA gene >50 bp long)

mitofish.12S.Dec2024_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Dec2024.tsv (318,682 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.14647420
title: Jan2025 reference datasets for Mitohelper

description: Reference datasets (Jan 2025 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Jan2025.tsv (880,055 records)

mitofish.12S.Jan2025.tsv (60,942 records of 12S rRNA gene >50 bp long)

mitofish.12S.Jan2025_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Jan2025.tsv (321,227 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.15028392
title: Mar2025 reference datasets for Mitohelper

description: Reference datasets (Mar 2025 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:



mitofish.all.Mar2025.tsv (883,519 records)

mitofish.12S.Mar2025.tsv (61,379 records of 12S rRNA gene >50 bp long)

mitofish.12S.Mar2025_NR.fasta (FASTA file of 12S rRNA gene records)

mitofish.COI.Mar2025.tsv (323,337 records)


II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:



12S-seqs-derep-uniq.qza

12S-tax-derep-uniq.qza

12S-16S-18S-seqs.qza

12S-16S-18S-tax.qza


If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.4257040
title: Nov2020 reference datasets for Mitohelper

description: <strong>Reference datasets (Nov 2020 update) for Mitohelper (<em>https://github.com/aomlomics/Mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Nov2020.tsv (660,961 records; Nov 2020 update) mitofish.12S.Nov2020.tsv (36,212 records) mitofish.COI.Nov2020.tsv (221,940 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4266949
title: Nov2020 reference datasets for Mitohelper

description: <strong>Reference datasets (Nov 2020 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Nov2020.tsv (660,961 records; Nov 2020 update) mitofish.12S.Nov2020.tsv (37,102 records) mitofish.COI.Nov2020.tsv (221,613 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4302541
title: Dec2020 reference datasets for Mitohelper

description: <strong>Reference datasets (Nov 2020 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Dec2020.tsv (660,976 records; Dec 2020 update) mitofish.12S.Dec2020.tsv (37,117 records) mitofish.COI.Dec2020.tsv (221,628 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4420495
title: Jan2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Jan 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Jan2021.tsv (668,366 records; Jan 2021 update) mitofish.12S.Jan2021.tsv (37,314 records) mitofish.COI.Jan2021.tsv (224,712 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4458571
title: Jan2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Jan 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Jan2021.tsv (668,366 records; Jan 2021 update) mitofish.12S.Jan2021.tsv (37,314 records) mitofish.COI.Jan2021.tsv (224,712 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4533286
title: Feb2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Feb 2021 update with bug fixes) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Feb2021.tsv (668,366 records; Jan 2021 MitoFish release) mitofish.12S.Feb2021.tsv (37,314 records) mitofish.COI.Feb2021.tsv (224,712 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
description: Fixed irregular column breaks in Jan 2021 reference dataset. No records added or removed compared to Jan 2021.
DOI: 10.5281/zenodo.4589660
title: Mar2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Mar 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Mar2021.tsv (681,971 records; Mar 2021 MitoFish release) mitofish.12S.Feb2021.tsv (37,841 records) mitofish.COI.Feb2021.tsv (229,905 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4663344
title: Apr2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Apr 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Apr2021.tsv (682,011 records; Apr 2021 MitoFish release without obsolete record MW387537 ) mitofish.12S.Apr2021.tsv (37,880 records) mitofish.COI.Apr2021.tsv (229,944 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
DOI: 10.5281/zenodo.4892036
title: Jun2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Jun 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Jun2021.tsv (691,892 records) mitofish.12S.Jun2021.tsv (38,086 records) mitofish.COI.Jun2021.tsv (233,759 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza If you use Mitohelper, please cite:<br> Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10. https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.5059742
title: Jul2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Jul 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Jul2021.tsv (701,894 records) mitofish.12S.Jul2021.tsv (38,451 records) mitofish.COI.Jul2021.tsv (239,330 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza If you use Mitohelper, please cite:<br> Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10. https://doi.org/10.1002/edn3.187
DOI: 10.5281/zenodo.5403427
title: Sep2021 reference datasets for Mitohelper

description: <strong>Reference datasets (Sep 2021 update) for Mitohelper (<em>https://github.com/aomlomics/mitohelper</em>)</strong> Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Sep2021.tsv (712,927 records) mitofish.12S.Sep2021.tsv (38,699 records) mitofish.COI.Sep2021.tsv (244,767 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza If you use Mitohelper, please cite:<br> Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10. https://doi.org/10.1002/edn3.187