DOI: 10.5061/dryad.p1b140p
title: Data from: Developmental exposure to PCB153 (2,2’,4,4’,5,5’-hexachlorobiphenyl) alters circadian rhythms and the expression of clock and metabolic genes

description: Polychlorinated biphenyls (PCBs) are highly persistent and ubiquitously
 distributed environmental pollutants. Based on their chemical structure,
 PCBs are classified into non-ortho-substituted and ortho-substituted
 congeners. Ortho-substituted PCBs are structurally similar to dioxin and
 their toxic effects and mode of action are well established. In contrast,
 very little is known about the effects of non-ortho-substituted PCBs,
 particularly during early development. The objective of this study is to
 investigate the effects of exposure to an environmentally prominent
 ortho-substituted PCB (2,2’,4,4’,5,5’-hexachlorobiphenyl; PCB153) on
 zebrafish embryos. We exposed zebrafish embryos to either DMSO or three
 different concentrations of PCB153 starting from 4 hours
 post-fertilization (hpf) to 120 hpf. We quantified gross morphological
 changes, behavioral phenotypes, gene expression changes and circadian
 behavior in the larvae. There were no developmental defects during the
 exposure period, but starting at 7 dpf, we observed spinal deformity in
 the 10 μM PCB153 treated group. A total of 633, 2227, and 3378
 differentially expressed genes were observed in 0.1, 1 and 10 μM PCB153
 treated embryos, respectively. Of these, 301 genes were common to all
 treatment groups. KEGG pathway analysis revealed enrichment of genes
 related to circadian rhythm, FoxO signaling and insulin resistance
 pathways. Behavioral analysis revealed that PCB153 exposure significantly
 alters circadian behavior. Disruption of circadian rhythms has been
 associated with the development of metabolic and neurological diseases.
 Thus, understanding the mechanisms of action on environmental chemicals in
 disrupting circadian regulation of metabolism is essential for preventing
 or mitigating chemical-induced metabolic disease.
description: Core_genesThese are the core set of genes (301) that are differentially expressed in all three PCB153 doses (0.1, 1 and 10 uM). Gene IDs (ensembl ID, zebrafish and human), fold change and FDR values are provided for each set.Gene Ontology and KEGG pathway analysis of differentially expressed genes.This file provides Gene Ontology (GO) and KEGG pathway analysis results. This includes summary of pathway analysis on all the differentially expressed genes (spread sheet 1), up and down regulated genes (sheet 2 and 3) and the core set of genes (sheet 4). The genes represented under each GO and KEGG pathway term are provided (sheet 2 and 3).GO_KEGG_dryad.xlsxList of differentially expressed genes in response to 0.1uM PCB153 exposure during developmentThis file contains complete list of genes as well as statistically significant (FDR &lt; 5%) gene set. In addition, up and downregulated genes are provided.Low-cont-results.xlsxList of differentially expressed genes in response to 1uM PCB153 exposure during developmentThis file contains complete list of genes as well as statistically significant (FDR &lt; 5%) gene set. In addition, up and downregulated genes are provided.Med-cont-results.xlsxList of differentially expressed genes in response to 10 uM PCB153 exposure during developmentThis file contains complete list of genes as well as statistically significant (FDR &lt; 5%) gene set. In addition, up and downregulated genes are provided.High-cont-results.xlsx