DOI: 10.15125/bath-00317
title: Data from "Dual-specificity phosphatase 5 (DUSP5) controls the localized inhibition, propagation and transforming potential of ERK signaling"

description: This repository contains the data used to construct figures in the paper "Dual-specificity phosphatase 5 (DUSP5) controls the localized inhibition, propagation and transforming potential of ERK signaling" by Kidger et al., published in Proceedings of the National Academy of Sciences, USA. All numerical data are included in a Microsoft Excel file labelled "numerical data for Kidger et al 2017". Tabs within this spreadsheet are labelled with the figures names the data correspond to in the publication. Similarly, images and Western blot scans are all labelled with figure titles. Please see the publication for details of experimental procedures.
description: Details of experimental procedures can be found in the Kidger et al. 2017 publication. Detailed high content microscopy methodology can also be found in the following paper:
Caunt, Kidger and Keyse, Methods Mol Biol. 2016;1447:197-215. doi: 10.1007/978-1-4939-3746-2_12.

Data provided in the Microsoft Excel file include a combination of data derived from high content microscopy and automated analysis (GE Healthcare IN Cell 2000 microscope and IN Cell Developer software), immunoblot analysis from scanned images using a Licor Odyssey scanner, quantitative PCR techniques and a small number of manual counting assays for cellular senescence and focus formation.
description: For high content microscopy, single cell data is shown as raw arbitrary fluorescence unit (AFU) values. For population-averaged data, where immunostaining experiments were pooled from several independent experiments, each of which usually had several replicate samples per plate, average AFU values from entire wells were normalised as percentages of values from control wells in each plate and applied across all technical replicate wells in the 96-well plate, prior to data pooling between independent experiments. Other types of data derived from high content microscopy are represented as discrete values. For example, an AFU cutoff for label intensity above background values was used to assign whether a cell had entered S-phase within a given pulse window of fluorescent labelling. All analysis of nuclear and cytoplasmic intensity values was carried out using a custom routine described in Caunt, Kidger and Keyse, Methods Mol Biol. 2016;1447:197-215. doi: 10.1007/978-1-4939-3746-2_12.

Where possible, we have included Western blot as original blot scans, as well as raw intensity values in the spreadsheet derived from the Licor scanner, and also values corrected for loading and normalised to control samples contained in each replicate blot (or normalised according to the mean signal intensity across the blot).
description: Please see Kidger et al 2017 for details of experimental procedures and equipment used.
description: Further relevant documentation may be found in the following resources.

   Kidger, A. M., Rushworth, L. K., Stellzig, J., Davidson, J., Bryant, C. J., Bayley, C., Caddye, E., Rogers, T., Keyse, S. M., and Caunt, C. J.,  2017.   Dual-specificity phosphatase 5 controls the localized inhibition, propagation, and transforming potential of ERK signaling.    Proceedings of the National Academy of Sciences, 114(3).  Available from: https://doi.org/10.1073/pnas.1614684114.