cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/eav9p-8gn94
title: E. coli QQQQ

description: Penicillin lysed E. coli QQQQ (grown in TB medium)
description: <strong>Tilt Series Date:</strong> 2015-03-05</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: 6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-05-8/rawdata/</p>QQQQMarTITAN0004.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-05-8/rawdata/QQQQMarTITAN0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        QQQQMarTITAN0004_full.rec,  Reconstruction (Pixel Size 1.08 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-03-05-8/3dimage_52563/QQQQMarTITAN0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tchfc-vbk96
title: 2014Apr13deltarem_27Cplant

description: grow on pure agar plate (just 15% agar, add bacterium drop on plant's root, 27C overnight, take plant for high pressure freeze and take some bacterium for cryo em tomography
description: <strong>Tilt Series Date:</strong> 2014-04-13</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-13-34/rawdata/</p>rem_plant20002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-13-34/rawdata/rem_plant20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        rem_plant20002_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-13-34/3dimage_41454/rem_plant20002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/174d9-12q67
title: wt B. subtilis sacculi

description: late sporulation
description: <strong>Tilt Series Date:</strong> 2012-09-08</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-41.0°, 41.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-08-11/rawdata/</p>late10005.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-08-11/rawdata/late10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        late10005_full.rec,  Reconstruction (Pixel Size 2.0 nm), 795.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-08-11/3dimage_20744/late10005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ywswm-ska25
title: S. aureus

description: Raw data files of S. aureus
description: <strong>Tilt Series Date:</strong> 2017-11-06</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-11-06-13/rawdata/</p>Sa_rpsF0013.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-11-06-13/rawdata/Sa_rpsF0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        Sa_rpsF0013.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-11-06-13/3dimage_90113/Sa_rpsF0013.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z8af6-1dk43
title: S. aureus

description: Raw data files of S. aureus
description: <strong>Tilt Series Date:</strong> 2017-11-07</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 230.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-11-07-28/rawdata/</p>Sa_rpsF_0028.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-11-07-28/rawdata/Sa_rpsF_0028.mrc"
        > <i class="download icon"></i> Download </a></p>

        Sa_rpsF_0028_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-11-07-28/3dimage_90233/Sa_rpsF_0028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y752g-mk360
title: purified BamA from E.coli

description: Raw data files of purified BamA from E.coli
description: <strong>Tilt Series Date:</strong> 2015-05-08</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 95.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-05-08-1/rawdata/</p>purifiedBamA_1.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-05-08-1/rawdata/purifiedBamA_1.mrc"
        > <i class="download icon"></i> Download </a></p>

        purifiedBamA_1_part131_7.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-05-08-1/3dimage_59267/purifiedBamA_1_part131_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2wqnq-bc989
title: Mycobacterium marinum

description: sporulating
description: <strong>Tilt Series Date:</strong> 2015-05-19</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Mycobacterium marinum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-05-19-5/rawdata/</p>Mmarinum0005.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-05-19-5/rawdata/Mmarinum0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        Mmarinum0005_part121_2.rec,  Reconstruction (Pixel Size 2.52 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-05-19-5/3dimage_57260/Mmarinum0005_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zspr0-mwa51
title: PW1307

description: PW 1307 Pseudomonas aeruginosa PAO1 delta CheA (F7)transposon mutant collection
description: <strong>Tilt Series Date:</strong> 2015-05-28</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-28-5/rawdata/</p>PW1307deltaCheA0008.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-28-5/rawdata/PW1307deltaCheA0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        PW1307deltaCheA0008_part121_2.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-28-5/3dimage_57504/PW1307deltaCheA0008_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cqv2y-6w280
title: PW1307

description: PW 1307 Pseudomonas aeruginosa PAO1 delta CheA (F7)transposon mutant collection
description: <strong>Tilt Series Date:</strong> 2015-05-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-29-28/rawdata/</p>PW1307deltaCheA0038.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-29-28/rawdata/PW1307deltaCheA0038.mrc"
        > <i class="download icon"></i> Download </a></p>

        PW1307deltaCheA0038_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-29-28/3dimage_57561/PW1307deltaCheA0038_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.n9jvqd
title: Occurrence Download

description: A dataset containing 9653 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Panthera onca"
 ]
}

 The dataset includes 9653 records from 303 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007233-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/zhehv-w7122
title: V. cholerae CF59 - ∆tcpB pMT5

description: Classical strain with ctxA deletion.Minor pilin tcpB knockout.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-06-17</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-17-22/rawdata/</p>20150617_AK08_10022.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-17-22/rawdata/20150617_AK08_10022.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150617_AK08_10022_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-17-22/3dimage_58352/20150617_AK08_10022_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ajk5j-ygj79
title: Thermococcus kodakarensis

description: flagella
description: <strong>Tilt Series Date:</strong> 2016-08-25</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo Kim and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in standard Tk medium with starch and sulfur for one day.</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the Tk strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-43/rawdata/</p>Tk_250820160043.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-43/rawdata/Tk_250820160043.mrc"
        > <i class="download icon"></i> Download </a></p>

        Tk_250820160043_part81_15.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-43/3dimage_78649/Tk_250820160043_part81_15.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tmvej-vp356
title: Myxococcus xanthus delta-pilB&T

description: Grid box YWC128Strain SA4300
description: <strong>Tilt Series Date:</strong> 2015-04-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-22-43/rawdata/</p>20150422_YWC128_20041.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-22-43/rawdata/20150422_YWC128_20041.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150422_YWC128_20041_part121_5.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-22-43/3dimage_54709/20150422_YWC128_20041_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/db8pr-9f495
title: V. cholerae RT4236 (refreshed)

description: Grid box AK15.Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-07-13</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 N1 pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Refreshed in fresh media right before freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: C-flat Cu 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-13/rawdata/</p>20150713_AK15_10013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-13/rawdata/20150713_AK15_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150713_AK15_10013_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-13/3dimage_59132/20150713_AK15_10013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/th5rv-3rs83
title: M. xanthus knockout minor pilins

description: Grid box YWC130Strain SA6815
description: <strong>Tilt Series Date:</strong> 2015-04-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-57/rawdata/</p>20150426_YWC130_10053.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
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        20150426_YWC130_10053_part121_4.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
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