cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/vrtpc-qpn07
title: pED208_pilated

description: E coli strain with plasmid pED208 overexpresses T4SS and F-like pili
description: <strong>Tilt Series Date:</strong> 2015-01-14</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were obtained from Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> o/n liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-66/rawdata/</p>pED208b0012.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-66/rawdata/pED208b0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED208b0012_part121_10.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-66/3dimage_50705/pED208b0012_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w2q06-85577
title: LBculture_wt

description: This is a LB culture of wt from a flask grown into stationary phase. The purpose was to to check that there are no nanowires  and nothing was seen in sem, and when we do cryo-em to ensure we see flagellum. This would ensure that flagellum can't be seen in sem. But we did see long wires in sem. Sahand opined that is because we grew them into stationary phase. I imaged these to capture nanowires since they were present in sem
description: <strong>Tilt Series Date:</strong> 2015-07-25</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Manual blotting Cu side glow discharged. Sample was put on opposite side and blotted. Similar to vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-25-10/rawdata/</p>lbculture_nanowirepresent_atlas10009.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-25-10/rawdata/lbculture_nanowirepresent_atlas10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        lbculture_nanowirepresent_atlas10009_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-25-10/3dimage_59508/lbculture_nanowirepresent_atlas10009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jsqye-m5n26
title: COS7 CHMP4B+VPS4DN

description: Cos7 cells transfected with CHMP4B & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2015-03-08</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells were grown on grids and transfected with Lipofectamine</p>
<strong>Sample Preparation:</strong> blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2015-03-08-22/rawdata/</p>15mar08_C301-1_AT3BT1_0014.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2015-03-08-22/rawdata/15mar08_C301-1_AT3BT1_0014.mrc"
        > <i class="download icon"></i> Download </a></p>

        15mar08_C301-1_AT3BT1_0014_full.rec,  Reconstruction (Pixel Size 2.0 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2015-03-08-22/3dimage_52871/15mar08_C301-1_AT3BT1_0014_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r1e58-6zz86
title: CLEM of TRIM5a-YFP in HeLa cells

description: HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads 1/300. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS
description: <strong>Tilt Series Date:</strong> 2015-01-21</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 209.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-3/rawdata/</p>tomgraphytest3.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-3/rawdata/tomgraphytest3.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest3_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-21-3/3dimage_51194/tomgraphytest3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.28606919.v6
title: HD-SC-1：Hyperspectral Dataset for Stone Cultural Relics

description: <b><i>The spectral range of the dataset spans from 400 to 1000 nm, with an image resolution of 2048×2046 pixels. Additionally, the dataset contains 233 visible light spatial features, 466 visible light color features, and six simple deterioration classification tables. The primary objective of this study is to enhance the recognition accuracy of the deterioration modes of stone cultural relics and to deepen the related mechanism research.</i></b><i>Due to the limitation of figshare space and transmission speed, some sample data were uploaded. The complete data is uploaded to Baidu ( Spectral :</i><i>https：//pan.baidu.com/s/10ZtYvtx8MFa32Vmz5j1V4A Extracting code：HDSC</i><i> ) and Ali ( Other :</i>https://www.alipan.com/t/LilDFHdljfLdFnKaFoVz<i> ) for storage and sharing. Total 282GB.</i><br>
DOI: 10.22002/09tes-vh081
title: T5_1693Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1693 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-04-02</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot.</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-04-02-10/rawdata/</p>cys1693_sq25_4sqs0006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-04-02-10/rawdata/cys1693_sq25_4sqs0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        cys1693_sq25_4sqs0006_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.x65err
title: Occurrence Download

description: A dataset containing 71635 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Stellaria palustris"
 ]
}

 The dataset includes 71635 records from 398 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002360-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dxfhhr
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Uroteuthis vossi"
 ]
}

 The dataset includes 3 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000888-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6mhzrr
title: Occurrence Download

description: A dataset containing 34981 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Bromus racemosus"
 ]
}

 The dataset includes 34981 records from 809 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000883-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c3fd3j
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Mixornis gularis",
 "Country is Chinese Taipei",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000889-250827131500795/datasets/export for details.
DOI: 10.15468/dl.h8rm99
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ligustrum japonicum",
 "Country is Lesotho",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000890-250827131500795/datasets/export for details.
DOI: 10.15468/dl.564dzq
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ligustrum japonicum",
 "Country is Libya",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000891-250827131500795/datasets/export for details.
DOI: 10.15468/dl.9g8udr
title: Occurrence Download

description: A dataset containing 23 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ligustrum japonicum",
 "Country is Mexico",
 "HasCoordinate is true"
 ]
}

 The dataset includes 23 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000892-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3xhz79
title: Occurrence Download

description: A dataset containing 2502 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aves",
 "GadmGid is IDN.22_1",
 "BasisOfRecord is Specimen",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 2502 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000893-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5472y2
title: Occurrence Download

description: A dataset containing 53334 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Andryala integrifolia"
 ]
}

 The dataset includes 53334 records from 967 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000800-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6rrkk9
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Burmeistera catulum Muchhala & Mashburn"
 ]
}

 The dataset includes 8 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0022637-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nwfpkp
title: Occurrence Download

description: A dataset containing 615 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Cochliomyia hominivorax (Coquerel, 1858)"
 ]
}

 The dataset includes 615 records from 35 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0022639-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29247041
title: EB_EVP_TCM_2024_SCOPUS_ DATA

description: EB_EVP_TCM_2024_SCOPUS_ DATA
DOI: 10.6084/m9.figshare.29247041.v1
title: EB_EVP_TCM_2024_SCOPUS_ DATA

description: EB_EVP_TCM_2024_SCOPUS_ DATA
DOI: 10.15468/dl.24dz9j
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Gabon",
 "TaxonKey is Dalbergia obliquifoliolata O.Lachenaud"
 ]
}

 The dataset includes 4 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0022640-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/t9kqs-tfw35
title: Xanthomonas WT

description: Xac WT pBRaφ pBRR
description: <strong>Tilt Series Date:</strong> 2019-12-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Carvalho and Farah lab sent the strain. Debnath Ghosal collected data</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in 2xTY with apt antibiotics, freeze cells next day after about 14 hour when they are in stationary phase.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 5 sec. ** Use 7 sec for next sessions.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-46/rawdata/</p>Xac_No1_TS69.mrc,  Tilt Series (Pixel Size 0.353 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-46/rawdata/Xac_No1_TS69.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_No1_TS69_full.rec,  Reconstruction (Pixel Size 1.412 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-46/3dimage_112422/Xac_No1_TS69_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q7y2z-ztk33
title: Shewanella oneidensis MR-1

description: Raw data files of Shewanella oneidensis MR-1
description: <strong>Tilt Series Date:</strong> 2018-05-22</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were prepared in chemostat (2 days) by Sahand from Moh's group in USC.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> These are fixed cells prepared in chemostat (2 days). Poorna has prepared these grids but they were not good (only few cells present per grid). I imaged them on the Polara to check if they have "iron cores" so that I send them to Sharon in Weizmann Institute.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-22-24/rawdata/</p>tilt16.mrc,  Tilt Series (Pixel Size 0.57 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-22-24/rawdata/tilt16.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt16_part48_10.rec,  Reconstruction (Pixel Size 2.28 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-22-24/3dimage_93597/tilt16_part48_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xd35t-6m924
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-01</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-39/rawdata/</p>Test_Pseudomonas_dfliF_39_fix.mrc,  Tilt Series (Pixel Size 0.37 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-39/rawdata/Test_Pseudomonas_dfliF_39_fix.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_39_fix_full.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vvgh4-ey449
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-343/rawdata/</p>JV9298_PD-1-3_314.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
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        JV9298_PD-1-3_314_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
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