DOI: 10.5061/dryad.280gb5mw1
title: Monosaccharide transporter OsMST6 is activated by transcription factor OsERF120 to enhance chilling tolerance in rice seedlings

description: Chilling stress caused by extreme weather is threatening global rice
 (Oryza sativa L.) production. Identifying components of the signal
 transduction pathways underlying chilling tolerance in rice would advance
 molecular breeding. Here, we report that OsMST6, which encodes a
 monosaccharide transporter, positively regulates the chilling tolerance of
 rice seedlings. The mst6 mutants showed hypersensitivity to chilling,
 while the OsMST6 overexpression lines were tolerant. During chilling
 stress, OsMST6 transported more glucose into cells to modulate sugar and
 ABA signal pathways. We showed that the transcription factor OsERF120
 could bind to the DRE/CRT element of the OsMST6 promoter and activate the
 expression of OsMST6 to positively regulate chilling tolerance.
 Genetically, OsERF120 was functionally dependent on OsMST6 when promoting
 chilling tolerance. In summary, OsERF120 and OsMST6 form a new downstream
 chilling regulatory pathway in rice in response to chilling stress,
 providing valuable findings for molecular breeding aimed at achieving
 global food security. The stored data is the analysis results of
 transcriptome data from ZH11 and mst6-1 after 0 hours and 4 hours
 of chilling treatment. These data form the basis for the transcriptome
 data visualization in the article.
description: The 14-day-old rice seedlings of ZH11 and
 <em>mst6-1</em>, incubated in the greenhouse with 28 °C/25 °C
 (10 h day/14 h night) cycles in Kimura B nutrient solution, were treated
 for 0 h and 4 h at 4 °C, and the whole seedlings were sampled for
 transcriptome sequencing with three biological replicates. 
 RNA-seq was performed at Anoroad Genome (Beijing, China). Clean
 reads were mapped referring to the rice genome (<em>Oryza
 sativa</em> IRGSP-1.0.38), using HISAT2 (Sirén<em> et
 al.</em>, 2014) with default parameters. The significant
 differentially expressed genes were determined using DESeq2 with |Fold
 change| ≥2, adjusted P value &lt;0.05. Gene ontology (GO) analysis was
 performed using DAVID.
description: # Monosaccharide transporter *OsMST6* is activated by transcription factor
 OsERF120 to enhance chilling tolerance in rice seedlings
 [https://doi.org/10.5061/dryad.280gb5mw1](https://doi.org/10.5061/dryad.280gb5mw1) We have submitted our data, which are the results of transcriptome data analysis for *mst6-1* (a mutant of the monosaccharide transporter *OsMST6*) and wild-type ZH11 rice seedlings. The 14-day-old rice seedlings of *mst6-1* and ZH11 were chill-treated for 0 h and 4 h at 4 °C, then the entire seedlings were sampled for transcriptome sequencing and analysis with three biological replicates. The data include the differentially expressed genes (**Dataset_1_DEGs_in_ZH11_and_mst6-1.xlsx**), the significantly enriched terms of KEGG (Kyoto Encyclopedia of Genes and Genomes) and GO (Gene Ontology) analysis results (**Dataset_2_KEGG_and_GO_terms.xlsx**), and enriched genes from the ABA-activated signaling pathway and PP2C family (**Dataset_3_Gene_information_of_ABA-activated_signaling_and_PP2Cs.xlsx**). ## Description of the data and file structure ### Dataset\_1\_DEGs\_in\_ZH11\_and\_mst6-1 All Differentially Expressed Genes (DEGs) in ZH11 (Sheet: DEGs in ZH11)and *mst6-1* (Sheet: DEGs in mst6-1)after four-hour chilling treatment, compared to 0 hours. * Gene: The gene ID annotated by RAP-DB * padj: The adjusted *P*-value * Up/Down: The DEGs were up- or down-regulated in ZH11 and *mst6-1* ### Dataset\_2\_ KEGG\_and\_GO\_terms Dataset 2 consists of the analysis results of the unique DEGs in ZH11 and *mst6-1* after 4 hours of chilling treatment on the [DAVID website](https://david.ncifcrf.gov/home.jsp), including: * The significant enriched KEGG terms (Sheet: KEGG of ZH11) and GO terms (Sheet: GO of ZH11) of unique up-regulated genes in ZH11 after 4 hours of chilling treatment * The significant enriched KEGG terms (Sheet: KEGG of mst6-1) and GO terms (Sheet: GO of mst6-1) of unique down-regulated genes in *mst6-1* after 4 hours of chilling treatment Each column name represents the following meaning:  * Terms: The name of GO or KEGG terms * ID: The identifiers of GO or KEGG terms in the database * Count: The number of significantly enriched genes in GO or KEGG terms * *P*-value: the significance of the enrichment of a set of genes in the GO term * Fold Enrichment: the ratio of the proportion of genes annotated with a specific GO term in the input gene set to the proportion of genes annotated with the same GO term in the background/reference gene set * Category: GO contains three ontologies, namely BP (Biological Process), MF (Molecular Function), and CC (Cellular Component) ### Dataset\_3\_Gene\_information\_of\_ABA-activated\_signaling\_and\_PP2Cs Gene information about abscisic acid-activated signaling pathway (Sheet: abscisic acid-activated genes)and PP2Cs genes (Sheet: PP2Cs) that were upregulated only in ZH11 after four-hour chilling treatment. * Gene: The gene ID annotated by RAP-DB * MSU_ID: The gene ID annotated by [MSU](http://rice.uga.edu/) * Name: The gene name * Foldchange: The expression changes after 4-hour chilling treatment, compared to 0 hour * padj: The adjusted *P*-value * Description: Predicted gene functions ## Sharing/Access information The data was derived from original RNA-seq data, which is available in the Genome Sequence Archive in the National Genomics Data Center and can be accessed with accession number CRA011327.