cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647550
title: CIL:24044

description: Starved cells of a chemotaxis-deficient mutant of Dictyostelium, KI-8 (lacking guanylyl cyclase activity) migrate toward the anode (right) with a slower migration velocity than that of wild-type cells (wild-type cells migrate toward the cathode). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) Compare with wild-type control video (CIL# 24043) and other mutants (CIL#s 24045, 24046). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647550.v1
title: CIL:24044

description: Starved cells of a chemotaxis-deficient mutant of Dictyostelium, KI-8 (lacking guanylyl cyclase activity) migrate toward the anode (right) with a slower migration velocity than that of wild-type cells (wild-type cells migrate toward the cathode). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) Compare with wild-type control video (CIL# 24043) and other mutants (CIL#s 24045, 24046). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647551.v1
title: CIL:24786

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image shows an overview of the leading edge, and CIL 24787 shows enlargements of local regions of this platinum replica. CIL 24788 shows a similar overview for the lamellipodia of a vertebrate fibroblasts. Image corresponds to Figure 1a from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647547
title: CIL:13895

description: DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with glucose. This is the control image for CIL# 13894 in which overexpression of Kin4 arrests cells in anaphase. Image is Fig 5C top left panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647548.v1
title: CIL:23051

description: MAP4 is not required for plectin binding to microtubules (MTs). Electron microscopy of control human 356 fibroblasts (CIL:23051, this image) and MAP4 immunodepleted (CIL:23052) cells after staining with antibody to NH 2 terminus of MAP4. Cells were microinjected with an antibody to the COOH-terminal domain of MAP4 to remove MAP4 from MTs. 10-nm gold particles decorate MTs in control cells, but are absent in injected cells. Plectin forms bridges between intermediate filaments and MTs in both cells. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 9c in J Cell Biol. 1996 Nov;135(4):991-1007.
DOI: 10.6084/m9.figshare.647545
title: CIL:11023

description: Transmission electron micrograph showing part of a decondensed mitotic chromosome. The residual protein 'scaffold' (at bottom) is surrounded by a 'halo' of DNA loops 20 to 24 micrometers long. The entire chromosome is seen in the grouped CIL image 11021.
DOI: 10.6084/m9.figshare.647546
title: CIL:13894

description: DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with galactose. Overexpression of Kin4 arrests cells in anaphase. Compare with control image CIL# 13895. Image is Fig 5C top right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647547.v1
title: CIL:13895

description: DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with glucose. This is the control image for CIL# 13894 in which overexpression of Kin4 arrests cells in anaphase. Image is Fig 5C top left panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647544
title: CIL:40667

description: Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B appears as CIL 40664. The uncolorized overview panel (this image) is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.
DOI: 10.6084/m9.figshare.647545.v1
title: CIL:11023

description: Transmission electron micrograph showing part of a decondensed mitotic chromosome. The residual protein 'scaffold' (at bottom) is surrounded by a 'halo' of DNA loops 20 to 24 micrometers long. The entire chromosome is seen in the grouped CIL image 11021.
DOI: 10.6084/m9.figshare.647546.v1
title: CIL:13894

description: DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with galactose. Overexpression of Kin4 arrests cells in anaphase. Compare with control image CIL# 13895. Image is Fig 5C top right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647541
title: CIL:40359

description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6084/m9.figshare.647542
title: CIL:40361

description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6084/m9.figshare.647543
title: CIL:40664

description: Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Yellow boxes are enlarged in panels with corresponding numbers. Box 4, interaction of actin filaments (cyan) with an axonal microtubule (red). Box 5, branched actin filaments (cyan) in the neck of respective spines. This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B (this image) appears as CIL 40664. The uncolorized overview panel is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.
DOI: 10.6084/m9.figshare.647543.v1
title: CIL:40664

description: Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Yellow boxes are enlarged in panels with corresponding numbers. Box 4, interaction of actin filaments (cyan) with an axonal microtubule (red). Box 5, branched actin filaments (cyan) in the neck of respective spines. This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B (this image) appears as CIL 40664. The uncolorized overview panel is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.
DOI: 10.6084/m9.figshare.647544.v1
title: CIL:40667

description: Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B appears as CIL 40664. The uncolorized overview panel (this image) is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.
DOI: 10.6084/m9.figshare.647539
title: CIL:24070

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This movie is a z-stack (0.3 µm) of images of a FRAP experiment on MCF7 cell junctions expressing E-cadherin-GFP immediately following photobleaching (CIL# 24069 shows z-series before photobleaching and CIL# 24068 shows time-lapse video of FRAP experiment). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647540
title: CIL:40358

description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks, and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6084/m9.figshare.647542.v1
title: CIL:40361

description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6084/m9.figshare.647540.v1
title: CIL:40358

description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks, and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6084/m9.figshare.647541.v1
title: CIL:40359

description: This fluorescent micrograph is part of an image group displaying the morphological changes associated with a developing oligodendrocyte. The progression of images starts with CIL:40358 where cells display a relatively bipolar morphology to CIL:40359 where the actin cytoskeleton becomes very prominent to CIL:40360 where the actin coalesces and interlinks and finally there is CIL:40361 where one can observe the formation of a flattened lamellar structure which in vitro mimics the myelin sheet.
DOI: 10.6084/m9.figshare.647536
title: CIL:24067

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This movie is a z-stack (0.3 µm) of images of a FRAP experiment on MDCK cell junctions expressing E-cadherin-GFP immediately following photobleaching (CIL# 24066 shows z-series before photobleaching and CIL# 24065 shows time-lapse video of FRAP experiment). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647537
title: CIL:24068

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This is the time-lapse video of a FRAP experiment on MCF7 cell junctions expressing E-cadherin-GFP. Also available are the z-stacks (0.3 µm) of images before (CIL# 24069) and immediately following photobleaching (CIL# 24070). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647538
title: CIL:24069

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This z-stack (0.3 µm) of images is of a FRAP experiment on MCF7 cell junctions expressing E-cadherin-GFP before photobleaching. Also available is the z-stack immediately following photobleaching (CIL# 24070) and the time-lapse video of FRAP experiment (CIL# 24068). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647539.v1
title: CIL:24070

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This movie is a z-stack (0.3 µm) of images of a FRAP experiment on MCF7 cell junctions expressing E-cadherin-GFP immediately following photobleaching (CIL# 24069 shows z-series before photobleaching and CIL# 24068 shows time-lapse video of FRAP experiment). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647535
title: CIL:24066

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This movie is a z-stack (0.3 µm) of images of a FRAP experiment on MDCK cell junctions expressing E-cadherin-GFP before photobleaching. Also available is a z-stack of images immediately following photobleaching (CIL# 24067) and the time-lapse video of FRAP experiment (CIL# 24065). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647536.v1
title: CIL:24067

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This movie is a z-stack (0.3 µm) of images of a FRAP experiment on MDCK cell junctions expressing E-cadherin-GFP immediately following photobleaching (CIL# 24066 shows z-series before photobleaching and CIL# 24065 shows time-lapse video of FRAP experiment). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647537.v1
title: CIL:24068

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This is the time-lapse video of a FRAP experiment on MCF7 cell junctions expressing E-cadherin-GFP. Also available are the z-stacks (0.3 µm) of images before (CIL# 24069) and immediately following photobleaching (CIL# 24070). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647538.v1
title: CIL:24069

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This z-stack (0.3 µm) of images is of a FRAP experiment on MCF7 cell junctions expressing E-cadherin-GFP before photobleaching. Also available is the z-stack immediately following photobleaching (CIL# 24070) and the time-lapse video of FRAP experiment (CIL# 24068). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647534
title: CIL:24065

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This is a time-lapse video of a FRAP experiment on MDCK cell junctions expressing E-cadherin-GFP (each image is a projection of 14 z-frames). Also available are z-stacks (0.3 µm) of images of the FRAP experiment before (CIL# 24066) and immediately following photobleaching (CIL# 24067). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647535.v1
title: CIL:24066

description: E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. After stable transfection with E-cadherin-GFP, E-cadherin dynamics were studied by 2-photon single-particle tracking and fluorescence recovery after photobleaching (FRAP) combined with 3D wide-field fluorescence microscopy, which allowed the recovery process to be analyzed from series of image stacks in the entire 3D region surrounding the photobleached volume. Image analysis of fluorescence recovery indicates that most E-cadherin did not diffuse in the membrane along mature junctions, but followed a first order turn-over process that was rate-limited by endocytosis. This movie is a z-stack (0.3 µm) of images of a FRAP experiment on MDCK cell junctions expressing E-cadherin-GFP before photobleaching. Also available is a z-stack of images immediately following photobleaching (CIL# 24067) and the time-lapse video of FRAP experiment (CIL# 24065). Photobleached regions are indicated by arrows. Scale bar: 10 µm.
DOI: 10.6084/m9.figshare.647531
title: CIL:39046

description: Colorized light micrograph of Aggregatibacter actinomycetemcomitans colonies. A. actinomycetemcomitans is a Gram-negative bacteria commonly found to cause infections of the oral cavity and often isolated from the periodontal pocket. The peridontium refers to the soft tissue that surrounds and supports to tooth. Related images available as CIL:39042, 39044, and 39045.