cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17639/nott.6741
title: An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 43808

description: This data set forms part of the larger 'Fly Cancer Screen' data set, which consists of over 700 image sets
description: Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’. RNAi line: 43808 (III) Source: VDRC Name: Or59b (1) Full name: Odorant receptor 59b Also known as: Annotation symbol: CG3569 FlyBase ID: FBgn0034865 File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)
DOI: 10.17639/nott.6823
title: An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 7305R2

description: This data set forms part of the larger 'Fly Cancer Screen' data set, which consists of over 700 image sets
description: Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’. RNAi line: 7305R2 (III) Source: NIG Name: Rim (1) Full name: Rab3 interacting molecule Also known as: Annotation symbol: CG33547 FlyBase ID: FBgn0053547 File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)
DOI: 10.17639/nott.6469
title: An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 8027R-3

description: This data set forms part of the larger 'Fly Cancer Screen' data set, which consists of over 700 image sets
description: Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’. RNAi line: 8027R-3 (III) Source: NIG Name: CG8027 Full name: Also known as: Annotation symbol: CG8027 FlyBase ID: FBgn0033392 File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)
DOI: 10.17639/nott.7332
title: EZInkjet code

description: Analytical model to predict free form inkjet-printed features using MATLAB
DOI: 10.17639/nott.7347
title: Serial 3T MRI in early Parkinson’s to validate PD progression markers: Rationale and MRI analysis plan for nigral depigmentation at baseline, 6- and 12-months’ follow-up

description: Access to these files may be requested. Please contact Dr Xing at You.Xing@nottingham.ac.uk. While NM-MRI has shown promise in diagnosing PD, its potential as a progression marker, particularly in the early stages when disease-modifying treatments are most effective, remains relatively unexplored. Our InsIghtPD study focuses on evaluating the substantia nigra, primarily looking at nigral depigmentation at baseline, 6- and 12-months’ follow-up. Secondary metrics, such as free water, kurtosis, and susceptibility, are also examined. Additionally, the study explores other whole brain metrics with potential as progression markers. The goal is to understand the minimal detectable change in these MRI metrics during the early clinical phase of PD. Furthermore, the study seeks to differentiate PD-related pathological changes from the effects of natural aging, which is critical for accurately assessing disease progression.
DOI: 10.17639/nott.7375
title: Excel database of screening from a systematic review looking at waiting list interventions for children and young people using child and adolescent mental health services

description: The data was obtained in a search of three databases for the systematic review and backwards and forwards citation chasing
DOI: 10.17639/nott.7357
title: DigiScore_Transdisciplinary_roundtable_6_Laura_Bishop

description: Roundtable discussion with Dr. Laura Bishop. Topic: How can we understand togetherness in ensemble performances with collaborative/ interactive/ intelligent digital scores? This was the sixth conversation in a series of trans-disciplinary discussions with experts from a range of fields to enhance the theoretical understanding of the digital score. The aim of this series is exploratory and openness so that we expose, rather than close, new insights that help us understand meaning-making in digital score creativity. Critical insights from this 1-2-1 are: - musical togetherness can be understood as being socially rewarding, and this can be split into two distinct areas: 1) close musical alignment, b) social connections - These are positive connections and meaningful from an emotional connection as well as musical competencies - musical togetherness tends to be focused on embodied relationships, such as shared rhythms, feelings, and synchronicity. - Key factors are: presence, ability to distinguish self and others as/ in sound, reliable relationships Digital scores can evoke a sense of togetherness by (in order of difficulty): 1. being responsive beyond rhythmic synchronicity such as dynamic, articulation, phrasing 2. sharing musical goals beyond sound parameters into musical ideas. Perhaps learning over time 3. showing itself to be creative; offering sensible motives, inspiration and playfulness 4. showing facets that are aligned to the natural musicality of humans; these may be familiar to inspire meaning-making. Challenges to the research: 1. Attention issues - does a digital score demand too much attention? Is it too verbose or trying to show too much? Does it take the musicians out of the music? 2. How does it segregate itself from the others? Can it integrate as a musician into the ensemble? 3. The digital score is a useful platform for testing out togetherness due to its disruption of many musical norms (scoring), controllability, and defined aesthetic ideas.
DOI: 10.17639/nott.7349
title: Tailoring the in-situ formation of intermetallic phases in the self-lubricating Al-WS2 composite for enhanced tribological performance with wear track evolution analysis

description: Self-lubricating aluminium matrix composites with enhanced tribological properties are sought for weight critical applications. In previous studies, the Al composites incorporating the solid lubricant WS2 have been shown to reduce both the coefficient of friction and wear rate, positioning them as promising candidates in various tribological applications (e.g. automotive industry). However, the impact of interfacial reactions between Al and WS2 during composite production on tribological performance has still not yet been explored. This study highlights the hardening effect of the reaction products. Despite some literature assuming a negative impact of these reactions as they consume WS2 in the composites, this study presents evidence that this cannot be generalised for the overall outcome. Interestingly, a controlled amount is shown to be beneficial for tribological properties. In this work, the tribological influence of the Al-W intermetallic structure forming during spark plasma sintering of the Al-WS2 composites was investigated. The microstructure was tailored by adjusting the manufacturing temperature between 500 and 600 °C. The Al-WS2 fabricated at 580 °C exhibited the lowest coefficient of friction and specific wear rate (reduced by 20% and 97%, respectively, compared to the one fabricated at 500 °C. Furthermore, the worn surface morphology in different stages during friction was evaluated by a novel wear track evolution analysis. This study confirmed that offering a balance between the fraction of solid lubricants and in-situ formed hard intermetallic structure is crucial to the effective formation a protective layer on the worn surface.
DOI: 10.17639/nott.7344
title: Heat and salinity stress on the African eggplant

description: This data was collected as part of the research on the heat and salinity stress on the African eggplant to describe the effects of the stress combination on this crop. The data was collected at East Malling, UK, and comprises harvest data, physiological measurements, and nutritional quality data.
DOI: 10.17639/nott.7286
title: Dataset of the Eczema Monitoring Online randomised controlled trial

description: The objectives of this study were to evaluate the effect of weekly patient-reported symptom monitoring on participants’ outcomes and to inform the design of future eczema trials on the optimum frequency of outcome assessments. Electronic patient-reported outcome measures were used. All trial processes occurred online, including participant recruitment, eligibility screening, consent, randomisation and data collection through the Research Electronic Data Capture (REDCap©, Vanterbilt University), which is a secure web platform for managing trial data. Recruitment took place through internet-based methods, mainly social media. Individuals who clicked on the advert link were directed to the study website at www.emostudy.org2, which included study aims, eligibility criteria and full participant information. Individuals interested in taking part enrolled through the website. Upon providing informed consent electronically and completing eligibility checks, participants were randomised. After enrolment, participants received an automated welcome email explaining the frequency of data collection, according to their randomised allocation. Email reminders were sent after follow-up questionnaires were overdue by 5 days and 7 days and if still incomplete, a final text reminder with a weblink to the questionnaires was sent.
DOI: 10.17639/nott.7351
title: RNA-seq data- 12 hrs and 24 hrs for C. beijerinckii NCIMB 8052 WT versus agrB mutants

description: RNA-seq data from three independent agrB mutants made in C. beijerinckii NCIMB 8052. Mutations were made in three different Agr systems, denoted by Agr2, Agr4 and Agr5. Total RNA was extracted from three replicates of each agrB mutant and the WT. RNA sequencing and analysis was carried out by DeepSeq (University of Nottingham). Mutants were compared to the WT gene expression at both 12 and 24 hour sampling points (Grown in glucose containing media).
DOI: 10.17639/nott.7330
title: Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis

description: Mass spectrometry data to support identification of the GGPPS enzyme
DOI: 10.17639/nott.7294
title: Red rot in leather

description: Data collected during a collaboration between the University of Nottingham and the Natural History Museum on the acidic deterioration process termed 'red rot' in leather book bindings. This includes photos of the books, samples, and spectroscopical set ups, as well as the spectroscopical data collected.
DOI: 10.17639/nott.7364
title: A novel diterpene synthase from the sandfly Lutzomyia longipalpis produces the pheromone sobralene

description: Raw mass spectrometry data in support of publication (submitted to PNAS). Abstract: The phlebotomine sandfly, Lutzomyia longipalpis, a major vector of the Leishmania parasite, uses terpene pheromones to attract conspecifics for mating. Examination of the L. longipalpis genome revealed a putative terpene synthase (TPS), which – upon heterologous expression in, and purification from, E. coli – yielded a functional enzyme. The TPS, termed LzTPS, converted geranyl diphosphate (GPP) into a mixture of monoterpenes with low efficiency, of which β-ocimene was the major product. (E,E)-farnesyl diphosphate (FPP) principally produced small amounts of (E)-β -farnesene, whilst (Z,E)- and (Z,Z)-FPP yielded a mixture of bisabolene isomers. None of these mono- and sesquiterpenes are known volatiles of L. longipalpis. Notably, however, when provided with (E,E,E)-geranylgeranyl diphosphate (GGPP), LzTPS gave sobralene as its major product. This diterpene pheromone is released by certain chemotypes of L. longipalpis, in particular those found in the Ceará state of Brazil. Minor diterpene-components were also seen as products of the enzyme that matched those seen in a sandfly pheromone extract.
DOI: 10.17639/nott.7374
title: Electrochemical Analysis of Gold Nanoparticles Multifunctionalised with Cytochrome c and a Zinc Porphyrin

description: Cytochrome c (Cyt c), known for its functional redox capabilities, plays a pivotal role in biological processes such as the electron transport chain and apoptosis. However, understanding how different conjugation strategies impact its structural and redox characteristics is limited. To fill this gap, we investigated the effects of conjugating Cyt c and a zinc(II) porphyrin (Zn Porph) to gold nanoparticles (AuNPs). We used circular dichroism (CD) spectroscopy to detect structural conformational changes in Cyt c upon conjugation and time-of-flight secondary ion mass spectrometry (TOF-SIMS) to identify protein orientation. Cyt c was predicted to have different orientations depending on the size of AuNPs and methods used to conjugate the protein, it was hypothesised that the orientation of Cyt c may influence the redox properties of the protein. The electrochemical properties of Cyt c were assessed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). We used DPV-based to determine the heterogeneous rate constant (k0). The results show a lower k° for conjugated Cyt c than free Cyt c, likely due to structural changes in the protein. The spatial orientation of Cyt c had minimal influence on k0, while ligand density and AuNP size had an effect. The k0 value of Zn Porph did not decrease on conjugation. Despite these changes, Cyt c and Zn Porph maintained their electrochemical capabilities after conjugation.
DOI: 10.17639/nott.7376
title: Metabolomic and proteomic analysis of ApoE4-carring H4 neuroglioma cells in Alzheimer’s disease using OrbiSIMS and LC-MS/MS

description: Raw and processed data used of metabolomics and proteomics in support of publication (submitted to Communication Biology).
DOI: 10.17639/nott.7371
title: Identification of factors that support return to work following work-related injury

description: This study sought to illuminate workers’ experience as they returned to work following a serious work injury and the co-worker, supervisor, and employer actions that supported their return. Workers in Saskatchewan, Canada, with a work-related psychological or musculoskeletal injury, subsequent disability, and returned to work in the last three years, were invited to complete an online survey comprising of free-text questions. This study was conducted in cooperation with the Saskatchewan Workers’ Compensation Board (SWCB). To be eligible to participate in the study a worker was required to have a ‘serious injury’, defined as a psychological injury or an injury with at least 50 days of partial or total disablity. They were also required to have returned to work following injury. All attended a rehabilitation program involving two to eight hours of intervention per day, for two to five days per week, and had been deemed ready to return to work by the rehabilitation provider and successfully returned to work between January 31, 2020 and January 31, 2023. The study received a favourable ethical opinion from the ethics sub-committee of the Mental Health and Clinical Neuroscience Academic Unit, School of Medicine, University of Nottingham, UK. The SWCB sent eligible individuals (N=2,035) a letter inviting their voluntary and anonymous participation. Informed consent to participate was provided by participants reading the SWCB invitation, accessing the survey website, reviewing the participant information sheet online and clicking the link to participate. No personally-identifiable information was solicited from participants.
DOI: 10.17639/nott.7370
title: Boson Star Superradiance

description: Data that was created in the paper https://arxiv.org/pdf/2306.01868.pdf for the study of radiation scattering off boson stars.
DOI: 10.17639/nott.7369
title: Quantum corrected Q-ball dynamics

description: The data stored here was generated in the paper https://arxiv.org/abs/2312.01139 and used for the figures in that paper.
DOI: 10.17639/nott.7355
title: Nigrosome depigmentation as seen on neuromelanin-sensitive MRI at 3T as diagnostic marker in clinical uncertain parkinsonism: analysis plan for the secondary aims of N3iPD study

description: Access to these files may be requested. Please contact Dr Xing at You.Xing@nottingham.ac.uk. In our prospective multi-centre MRI study at 3T, our primary aim was to establish the prospective diagnostic value of iron-sensitive susceptibility-weighted MRI of the substantia nigra in clinical uncertain Parkinsonism. We predefined depigmentation measured by neuromelanin-sensitive MRI scans (Trial registration number NCT03022357) as a secondary aim. This document details the research questions, hypotheses and analysis plan related the neuromelanin-MRI.
DOI: 10.17639/nott.7372
title: Teacher education for inclusive education in the Global South bibliography

description: This dataset comprises 95 journal articles published 2012 - 2021
DOI: 10.17639/nott.7367
title: Supplementary Material: Quantification of the effect of in-utero events on lifetime resilience in dairy cows

description: Supplementary material for "Quantification of the effect of in-utero events on lifetime resilience in dairy cows"
DOI: 10.17639/nott.7337
title: Fold by Ted Moore, digiscore and browser festival commission
title: Fold by Ted Moore

description: Fold by Ted Moore reflects the theme of “location sharing” by using audio feedback to “sound out” the resonant frequencies of the locations of the remote collaborators like Curt Miller in Rhode Island, US and Jana de Troyer in Ghent, Belgium. Using the resonant tubes of their instruments, the performers created and manipulated audio feedback tones in their remote locations. These sounds were broadcast over the internet to the performance space where the audience was present in Stuttgart, Germany. The feedback was generated in the remote locations through a feedback control patch in Max/MSP. Additionally, each remote performer’s sound was analyzed for pitch-class content and frequency content and sent to the performance space (over the internet using a Max package called Colab-Hub) as data, directing the pianist’s playing. The pianist’s gestures were precomposed as rhythms and registrar shapes, but the pitches were mostly supplied by the remote analyses of audio feedback. The hope was that the physical properties of the remote performers’ instruments and spaces would manifest in the performance space as acoustic sound filtered through the piano part. Overall the piece is slow and meditative, inviting everyone to enjoy the sonic relationships between the feedback and the piano. The form of the piece contains different sections where Curt and Jana are doing different things (and we can or cannot see them visually on the projection). Each has a different score indicating when to create feedback vs. performing tones. Also, a pre-composed piano part appears about two-thirds of the way through the performance, grounding the piece and the listeners in the presence of Alex’s expressive piano playing.
DOI: 10.17639/nott.7368
title: Protocol - modelling predictors for relapsing disease in MOGAD
title: Modelling predictors of relapsing adult MOGAD

description: In myelin oligodendrocyte glycoprotein antibody associated disease (MOGAD), only approximately 40%-60% of patients develop a relapsing disease course, for which the preventative treatment is long-term immunotherapy. Due to its significant adverse effect burden, long-term immunosuppression should generally be reserved only for patients likely to relapse, but there are currently no widely accepted predictors of developing relapsing MOGAD. A recent literature search published in February 2023 found no predictors of relapse in paediatric MOGAD. Identifying the predictive value of the combined risk factors for developing relapsing disease is of direct interest for the patient and for the treating neurologist, who needs to make the decision to commence maintenance therapy in MOGAD. Equally, quantifying the degree of uncertainty of prediction is important in the transparent communication with the patient, who needs to make an informed decision regarding the treatment. Studies in literature have most often studied predictors of relapse individually, but not combined. Having completed a scoping review of the available literature on predictors of relapsing MOGAD, our planned data analysis will aim to test the value of a model including the relevant predictors of developing relapsing MOGAD in adult patients with MOGAD (with onset in adulthood or childhood), using an observational data set (clinical data of adult patients with MOGAD – NMOSD&MOGAD Clinic, Nottingham Centre for MS and Neuroinflammation) and test the resulting model in an independent dataset sample (clinical data of adult MOGAD patients, the NMOSD Excellence Service, Walton Centre Liverpool).