DOI: 10.6084/m9.figshare.28003703.v1
title: Table S1, Table S2, Table S3

description: <b>Supplemental Tables for manuscript "</b><b>Cardiac Health, Type I Collagen, and Aging in the </b><b><i>oim/oim</i></b><b> Mouse Model of Osteogenesis Imperfecta (OI) and a Cohort of Adults with OI"</b><b>Methodology</b><b>Supplemental Table S1: 7T MRI - </b>Cardiac cine MRI was performed using a 7T/20cm MRI (Bruker BioSpin, Billerica, MA, USA) equipped with a cardiac phased-array radiofrequency (RF) coil. Mice were anesthetized with 1-2.5% isoflurane in oxygen via a nose cone at 1 mL/min. Respiratory rate was monitored to ensure adequate breathing at a rate of 30-35 breaths per minute. LV structural and functional data were acquired and analyzed as previously described<sup>1</sup>. Briefly, five to six short-axis slices were imaged across the length of the LV from the apex to the base for each heart. Twenty cine frames representing 20 phases of a cardiac cycle were captured using an IgFLASH (IntraGate Fast Low Angle Shot) sequence with an in-plane resolution of 156 × 156 µm<sup>2</sup> and a slice thickness of 1 mm. The LV endocardial and epicardial segmentations were performed by two operators using Segment software (Medviso, AB, Lund, Sweden).The LV systolic and diastolic functions and LV morphology were analyzed according to previously published methods: LV stroke volume (SV) = end diastolic volume (EDV) – end systolic volume (ESV); ejection fraction (EF) = SV/EDV × 100%; cardiac output (CO) = SV × heart rate (HR); fractional shortening (FS) = [end diastolic diameter (EDD) – end systolic diameter (ESD)] / EDD × 100% at mid-ventricle slice<sup>1-3</sup>. Further, anterior septal wall (ASW) thickness and posterior lateral wall (PLW) thicknesses were measured at the septum wall and free wall section on the mid-ventricle slice at end of diastole (ED), respectively. LV myocardium weight (LVW) was calculated by measuring the LV myocardial volume (LV myocardial volume = the epicardial volume – the endocardial volume at the ED) and multiplied by the tissue density of 1.055g/mL. The LV diastolic functions including diastolic peak filling rate (PFR), initial filling rate (IFR), and relaxation time (RT) were measured based on the LV volume (LVV)-time curve(35, 36). LVV-time curve was plotted as LVV versus time in one cardiac cycle. PFR was defined as the maximum derivative of the LVV-time curve. IFR was calculated by the slope of the first four time points on the early diastole. RT was defined as the time duration from the end systole to the PFR phase.<b>Supplemental Table S2: Survival outcomes under isoflurane anesthesia (</b><b>Death Events): </b>To monitor the potential effects of anesthesia on the <i>oim/oim</i> mice, any mouse that died while undergoing testing involving the use of isoflurane anesthesia was documented.<b>Supplemental Table S3: Electrocardiographs (ECGs) of 4-month-old male and female Wt and </b><b><i>oim/oim</i></b><b> mice</b><b>: </b><b> </b>ECGs were recorded using an ECGenie (Mouse Specifics Inc., Framingham, MA, USA). Mice were conscious and approximately 20 frames of two to ten seconds were recorded and analyzed using Emouse software (Mouse Specifics) to obtain conductance parameters, heart rate, and heart rate variability.<sup>1</sup>Lee LE, Chandrasekar B, Yu P, and Ma L. Quantification of myocardial fibrosis using noninvasive T2-mapping magnetic resonance imaging: Preclinical models of aging and pressure overload. <i>NMR Biomed </i>35: e4641, 2022.<sup>2</sup>Zhang H, Morgan B, Potter BJ, Ma L, Dellsperger KC, Ungvari Z, and Zhang C. Resveratrol improves left ventricular diastolic relaxation in type 2 diabetes by inhibiting oxidative/nitrative stress: in vivo demonstration with magnetic resonance imaging. <i>Am J Physiol Heart Circ Physiol </i>299: H985-994, 2010.<sup>3</sup>Ma L, Gul R, Habibi J, Yang M, Pulakat L, Whaley-Connell A, Ferrario CM, and Sowers JR. Nebivolol improves diastolic dysfunction and myocardial remodeling through reductions in oxidative stress in the transgenic (mRen2) rat. <i>A</i><i>m J Physiol Heart Circ Physiol </i>302: H2341-2351, 2012.