cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5671192
title: EFFECT OF PLATELET-RICH PLASMA GEL ON GRAFT SKIN HEALING IN EQUINES

description: Abstract The platelet-rich plasma (PRP) gel is an autologous platelet concentration in a small plasma volume with the potential to improve the integration of the grafts. Eight adult equines were used, performing skin grafts, with 2.5x2.5 centimeters, on both sides of the neck, in which one was the treatment side, using the PRP gel, and the other side was the control. The animals were assessed after the surgery on days 0, 7, 14, 21, and 28, by clinical, histological, morphometric, and culture analysis of the grafts. The clinical, morphological, and biological analysis of tissue grafts revealed no significant differences between the control group and the treatment group with PRP. Histologic evaluation revealed an increase in acute inflammation on day 7 and collagen concentration on day 14, resulting from the release of growth factors by the platelets present in the gel, which did not differ in further moments. According to the experimental conditions used in this study, we concluded there were no significant differences between the side treated with PRP and the control side in skin grafts in horses.
DOI: 10.6084/m9.figshare.7510298
title: EFFECT OF TEMPERATURE AND STORAGE TIME ON CANINE SERUM AND PLASMA SAMPLES IN THE ANALYSIS OF ENZYME ALANINE AMINOTRANSFERASE (ALT)

description: Abstract This study aimed to evaluate the effect of time and storage temperature on the values of ALT biochemical measurements in 60 samples of serum and plasma of dogs divided into two groups: RG (refrigerated group, at 5 ºC) and FG (frozen group, at -20 ºC) for 96 hours analyzed at six moments and up to 60 days examined at five moments, respectively. The study showed that the effect of freezing and cooling caused no variation between the samples (p &lt;0.005). The data showed that the biochemical serum and EDTA plasma remained near baseline, i.e., they did not change significantly over time and at different storage temperatures.
DOI: 10.6084/m9.figshare.7510298.v1
title: EFFECT OF TEMPERATURE AND STORAGE TIME ON CANINE SERUM AND PLASMA SAMPLES IN THE ANALYSIS OF ENZYME ALANINE AMINOTRANSFERASE (ALT)

description: Abstract This study aimed to evaluate the effect of time and storage temperature on the values of ALT biochemical measurements in 60 samples of serum and plasma of dogs divided into two groups: RG (refrigerated group, at 5 ºC) and FG (frozen group, at -20 ºC) for 96 hours analyzed at six moments and up to 60 days examined at five moments, respectively. The study showed that the effect of freezing and cooling caused no variation between the samples (p &lt;0.005). The data showed that the biochemical serum and EDTA plasma remained near baseline, i.e., they did not change significantly over time and at different storage temperatures.
DOI: 10.6084/m9.figshare.5671198
title: IDENTIFICATION OF Helicobacter spp. ON GASTRIC AND DUODENAL MUCOSA OF DOGS (Canis familiaris) BY WARTHIN-STARRY TECHNIQUE

description: Abstract Bacteria of the genus Helicobacter spp. have been identified on the gastric mucosa of different species of mammals, including canines. Infected patients have histological gastritis; however, not all the animals that carry the bacteria show clinical signs. The objective of this work was to identify the bacteria suggestive of Helicobacter spp. by impregnation by silver and to characterize the histopathological lesions observed. Qualitative evaluation was performed for histological analysis, in which scores from absent (-) to severe (+++) were attributed, considering the density of spiral bacteria per field (400x), presence of inflammatory cells, and number of lymphoid aggregates. Of the 26 animals evaluated, 34.6% presented positivity for helicobacteriosis. Gastritis was observed in 15.38% of the animals and a higher occurrence of bacteria of the genus Helicobacter spp. was observed in the gastric body/fundus region (23%). In nine samples, six from gastric body/fundus and three from antrum pylorus, lymphoid clusters associated with Helicobacter spp. were observed. Fisher's Test revealed a positive association between the presence of Helicobacter spp. and lymphoid aggregates (p&lt;0.001). Infection by Helicobacter spp. is associated with the presence of lymphoid aggregates in canines.
DOI: 10.6084/m9.figshare.5671198.v1
title: IDENTIFICATION OF Helicobacter spp. ON GASTRIC AND DUODENAL MUCOSA OF DOGS (Canis familiaris) BY WARTHIN-STARRY TECHNIQUE

description: Abstract Bacteria of the genus Helicobacter spp. have been identified on the gastric mucosa of different species of mammals, including canines. Infected patients have histological gastritis; however, not all the animals that carry the bacteria show clinical signs. The objective of this work was to identify the bacteria suggestive of Helicobacter spp. by impregnation by silver and to characterize the histopathological lesions observed. Qualitative evaluation was performed for histological analysis, in which scores from absent (-) to severe (+++) were attributed, considering the density of spiral bacteria per field (400x), presence of inflammatory cells, and number of lymphoid aggregates. Of the 26 animals evaluated, 34.6% presented positivity for helicobacteriosis. Gastritis was observed in 15.38% of the animals and a higher occurrence of bacteria of the genus Helicobacter spp. was observed in the gastric body/fundus region (23%). In nine samples, six from gastric body/fundus and three from antrum pylorus, lymphoid clusters associated with Helicobacter spp. were observed. Fisher's Test revealed a positive association between the presence of Helicobacter spp. and lymphoid aggregates (p&lt;0.001). Infection by Helicobacter spp. is associated with the presence of lymphoid aggregates in canines.
DOI: 10.6084/m9.figshare.7507013
title: WORM RESISTANCE AND PROTEIN SUPPLEMENTATION IN PERIPARTUM: EFFECT ON PARASITISM AND REPRODUCTIVE PERFORMANCE OF SHEEP FROM PANTANEIRO BREED GROUP

description: Abstract The objectives of this study were to evaluate the effect of the level of worm resistance and protein supplementation on reproductive performance and parasitism in Pantaneiro sheep. Sheep were classified by the number of nematode eggs per gram of feces (EPG) as resistant (RR), sensitive (SS), and intermediate (RS), as well as submitted (SUPL) or not (NSUPL) to supplementation during the final third of pregnancy and lactation. In both reproductive cycles, sheep were weighed every 28 days and treated with anthelmintic when EPG was ≥ 4000. The weight of the sheep was not affected by the degree of resistance to nematodes and supplementation only affected weight in the period in which it was offered. Birth and weaning rates were not affected by the degree of resistance and supplementation. Offspring mortality rate was lower in the SUPL group. Sheep mortality rate was three times higher in the SS group than in the RR group and offspring weight was lower in the SS group than in RR and RS groups. The three groups showed peaks in EPG during the final third of pregnancy; however, the averages were always lower in the RR group, which, in general, required 2.5 times less anthelmintic treatments than SS group. Supplementation had no effect on EPG and on the number of anthelmintic treatments. The classification of sheep as resistant to nematodes by EPG led to fewer anthelmintic treatments, lower sheep mortality rate and higher birth weight of offspring.
DOI: 10.6084/m9.figshare.7507013.v1
title: WORM RESISTANCE AND PROTEIN SUPPLEMENTATION IN PERIPARTUM: EFFECT ON PARASITISM AND REPRODUCTIVE PERFORMANCE OF SHEEP FROM PANTANEIRO BREED GROUP

description: Abstract The objectives of this study were to evaluate the effect of the level of worm resistance and protein supplementation on reproductive performance and parasitism in Pantaneiro sheep. Sheep were classified by the number of nematode eggs per gram of feces (EPG) as resistant (RR), sensitive (SS), and intermediate (RS), as well as submitted (SUPL) or not (NSUPL) to supplementation during the final third of pregnancy and lactation. In both reproductive cycles, sheep were weighed every 28 days and treated with anthelmintic when EPG was ≥ 4000. The weight of the sheep was not affected by the degree of resistance to nematodes and supplementation only affected weight in the period in which it was offered. Birth and weaning rates were not affected by the degree of resistance and supplementation. Offspring mortality rate was lower in the SUPL group. Sheep mortality rate was three times higher in the SS group than in the RR group and offspring weight was lower in the SS group than in RR and RS groups. The three groups showed peaks in EPG during the final third of pregnancy; however, the averages were always lower in the RR group, which, in general, required 2.5 times less anthelmintic treatments than SS group. Supplementation had no effect on EPG and on the number of anthelmintic treatments. The classification of sheep as resistant to nematodes by EPG led to fewer anthelmintic treatments, lower sheep mortality rate and higher birth weight of offspring.
DOI: 10.6084/m9.figshare.7510325
title: ORIGIN AND DISTRIBUTION OF THE CELIAC ARTERY IN YELLOW-TOOTHED CAVIES (Galea spixii Wagler, 1831)

description: Abstract For this study we used 20 animals that were in the freezer at the Wild Animals Multiplication Center (CEMAS/UFERSA). The animals were thawed, the abdominal cavity was opened, and the thoracic aorta cannulated caudally for the injection of red-stained latex Neoprene 650, and then fixed in formaldehyde solution 10%, for a minimum of 48 hours. Then the animals were dissected, photographed, and the results analyzed. The celiac artery originated from the abdominal aorta and issued the left gastric, splenic, and hepatic arteries. The left gastric artery issued two to three branches to the lesser curvature of the stomach. The splenic artery emitted four branches to the parenchyma of the spleen, six to twelve pancreatic branches and the left gastroepiploic artery. The hepatic artery emitted from one to two pancreatic branches and forked in a short trunk formed by right gastric and own hepatic arteries and in the gastroduodenal artery, which originated the cranial pancreaticoduodenal and right gastroepiploic. The trifurcation behavior of the celiac artery of the yellow-toothed cavy is similar to that found in rodents such as nutria, agouti, hamsters and mice, suggesting that this is the standard for the order.
DOI: 10.6084/m9.figshare.7510325.v1
title: ORIGIN AND DISTRIBUTION OF THE CELIAC ARTERY IN YELLOW-TOOTHED CAVIES (Galea spixii Wagler, 1831)

description: Abstract For this study we used 20 animals that were in the freezer at the Wild Animals Multiplication Center (CEMAS/UFERSA). The animals were thawed, the abdominal cavity was opened, and the thoracic aorta cannulated caudally for the injection of red-stained latex Neoprene 650, and then fixed in formaldehyde solution 10%, for a minimum of 48 hours. Then the animals were dissected, photographed, and the results analyzed. The celiac artery originated from the abdominal aorta and issued the left gastric, splenic, and hepatic arteries. The left gastric artery issued two to three branches to the lesser curvature of the stomach. The splenic artery emitted four branches to the parenchyma of the spleen, six to twelve pancreatic branches and the left gastroepiploic artery. The hepatic artery emitted from one to two pancreatic branches and forked in a short trunk formed by right gastric and own hepatic arteries and in the gastroduodenal artery, which originated the cranial pancreaticoduodenal and right gastroepiploic. The trifurcation behavior of the celiac artery of the yellow-toothed cavy is similar to that found in rodents such as nutria, agouti, hamsters and mice, suggesting that this is the standard for the order.
DOI: 10.6084/m9.figshare.7510334
title: CRANIOMETRIC MEASUREMENTS IN MANED WOLVES Chrysocyon brachyurus ILLIGER, 1815 (CARNIVORA, CANIDAE)

description: Abstract The aim of this study was to obtain craniometric measures in adult Chrysocyon brachyurus skulls, establish craniometric values for the species, and determine if the craniometric classification of domestic dogs is suitable for the maned wolf. Seven skulls of adult maned wolves were used, with no gender distinction. Nine craniometric points used in domestic dogs were used for the maned wolf. The points served as reference to 18 craniometric measurements used subsequently to calculate six skull indices. Craniometric indeces were: cefalic 96.147 ± 3.89 mm, craniofacial 1.06 ± 0.20 mm, neurocranium 61.68 mm ± 1.83, facial 100.06 ± 3.36 mm, basal 29.79 ± 1.18 mm, and foramen magnum index of 89.25 ± 7.82 mm. The usual classification in brachicephalic, dolichocephalic, and mesaticephalic is not valid for Chrysocyon brachyurus.
DOI: 10.6084/m9.figshare.7510334.v1
title: CRANIOMETRIC MEASUREMENTS IN MANED WOLVES Chrysocyon brachyurus ILLIGER, 1815 (CARNIVORA, CANIDAE)

description: Abstract The aim of this study was to obtain craniometric measures in adult Chrysocyon brachyurus skulls, establish craniometric values for the species, and determine if the craniometric classification of domestic dogs is suitable for the maned wolf. Seven skulls of adult maned wolves were used, with no gender distinction. Nine craniometric points used in domestic dogs were used for the maned wolf. The points served as reference to 18 craniometric measurements used subsequently to calculate six skull indices. Craniometric indeces were: cefalic 96.147 ± 3.89 mm, craniofacial 1.06 ± 0.20 mm, neurocranium 61.68 mm ± 1.83, facial 100.06 ± 3.36 mm, basal 29.79 ± 1.18 mm, and foramen magnum index of 89.25 ± 7.82 mm. The usual classification in brachicephalic, dolichocephalic, and mesaticephalic is not valid for Chrysocyon brachyurus.
DOI: 10.6084/m9.figshare.7514681
title: PLANKTON DENSITY IN Litopenaeus vannamei AND Oreochromis niloticus POLYCULTURE

description: Abstract An experiment was conducted during 95 days to evaluate plankton densities in polyculture of Pacific white shrimp Litopenaeus vannamei and Nile tilapia Oreochromis niloticus. The experiment design was randomized with three treatments and four replicates each. The treatments were as follows: monoculture with 10 shrimps m2 (10S:0F); polyculture with 10 shrimps and 0.5 fish m2 (10S:0.5F); polyculture with 10 shrimps and 1 fish m2 (10S:1F); monoculture with 2 fishes m2 (2F:0S); polyculture with 2 fishes and 2.5 shrimps m2 (2F:2.5S); and polyculture with 2 fishes and 5 shrimps m2 (2F:5S). Neither water exchange nor aeration was provided during the experimental period, only water was used to complete the volume lost by evaporation. The phytoplankton ranged from 78,981 to 303,260 cells.mL-1 and the zooplankton ranged from 470 to 1,421 org.L-1. The most frequent groups were Bacillariophyta (phytoplankton) and Rotifera (zooplankton). In conclusion, in the polyculture system of Pacific white shrimp Litopenaeus vannamei and Nile tilapia Oreochromis niloticus there are no significant changes in the densities of phytoplankton and zooplankton due to the alteration in the culture stocking density.
DOI: 10.6084/m9.figshare.7514681.v1
title: PLANKTON DENSITY IN Litopenaeus vannamei AND Oreochromis niloticus POLYCULTURE

description: Abstract An experiment was conducted during 95 days to evaluate plankton densities in polyculture of Pacific white shrimp Litopenaeus vannamei and Nile tilapia Oreochromis niloticus. The experiment design was randomized with three treatments and four replicates each. The treatments were as follows: monoculture with 10 shrimps m2 (10S:0F); polyculture with 10 shrimps and 0.5 fish m2 (10S:0.5F); polyculture with 10 shrimps and 1 fish m2 (10S:1F); monoculture with 2 fishes m2 (2F:0S); polyculture with 2 fishes and 2.5 shrimps m2 (2F:2.5S); and polyculture with 2 fishes and 5 shrimps m2 (2F:5S). Neither water exchange nor aeration was provided during the experimental period, only water was used to complete the volume lost by evaporation. The phytoplankton ranged from 78,981 to 303,260 cells.mL-1 and the zooplankton ranged from 470 to 1,421 org.L-1. The most frequent groups were Bacillariophyta (phytoplankton) and Rotifera (zooplankton). In conclusion, in the polyculture system of Pacific white shrimp Litopenaeus vannamei and Nile tilapia Oreochromis niloticus there are no significant changes in the densities of phytoplankton and zooplankton due to the alteration in the culture stocking density.
DOI: 10.6084/m9.figshare.7510340.v1
title: BETA DIVERSITY OF BIRDS (Passeriformes, Linnaeus, 1758) IN SOUTHERN AMAZON

description: Abstract The importance of estimating the biological diversity and understanding how ecological specialization of species changes with spatially-organized habitats in undeniable. High beta diversity between neighboring places means an elevated number of species living within small distances, which usually are adapted to local conditions and highly vulnerable to anthropogenic actions as deforestation and burning. We investigated beta diversity of birds from the order Passeriformes in Southern Amazon, within landscapes with a large heterogeneous vegetation cover (habitats with flooded forest, dry forest, and marsh palm) through sound, observational, and photographic censuses. We marked 126 points in equidistant transects. A total of 148 species of birds were identified, distributed in 27 families. We found that 97% of the species foraged in flooded forest, 77% in dry forest and 19% in marsh palms, and only 18% foraged in the three habitats. An ordination analyses revealed which species showed the strongest preference to each habitat. The analysis for the Global beta diversity showed that this value is high (Whittaker: 7.7405), and the same pattern was obtained with the measure of pairwise dissimilarity. An influence of spatial distance was clearly observed in the cluster analysis and confirmed with a partial Mantel analysis; however, this was not observed at points that coincided with the transition and substitution of species. The influence of spatial distance in the dissimilarity index (beta diversity) was significant (r: 0.0608, p: 0.0049). The assemblage of species in small local populations with high beta diversity may be at risk if deforestation, selective logging, and poaching continue. In summary, this study provides important information on specific habitats with high beta diversity that may be at risk of destabilization of its populations due to continuing environmental changes imposed by humans.
DOI: 10.6084/m9.figshare.7510340
title: BETA DIVERSITY OF BIRDS (Passeriformes, Linnaeus, 1758) IN SOUTHERN AMAZON

description: Abstract The importance of estimating the biological diversity and understanding how ecological specialization of species changes with spatially-organized habitats in undeniable. High beta diversity between neighboring places means an elevated number of species living within small distances, which usually are adapted to local conditions and highly vulnerable to anthropogenic actions as deforestation and burning. We investigated beta diversity of birds from the order Passeriformes in Southern Amazon, within landscapes with a large heterogeneous vegetation cover (habitats with flooded forest, dry forest, and marsh palm) through sound, observational, and photographic censuses. We marked 126 points in equidistant transects. A total of 148 species of birds were identified, distributed in 27 families. We found that 97% of the species foraged in flooded forest, 77% in dry forest and 19% in marsh palms, and only 18% foraged in the three habitats. An ordination analyses revealed which species showed the strongest preference to each habitat. The analysis for the Global beta diversity showed that this value is high (Whittaker: 7.7405), and the same pattern was obtained with the measure of pairwise dissimilarity. An influence of spatial distance was clearly observed in the cluster analysis and confirmed with a partial Mantel analysis; however, this was not observed at points that coincided with the transition and substitution of species. The influence of spatial distance in the dissimilarity index (beta diversity) was significant (r: 0.0608, p: 0.0049). The assemblage of species in small local populations with high beta diversity may be at risk if deforestation, selective logging, and poaching continue. In summary, this study provides important information on specific habitats with high beta diversity that may be at risk of destabilization of its populations due to continuing environmental changes imposed by humans.
DOI: 10.6084/m9.figshare.5671027
title: Escherichia coli O157:H7 SURVIVAL IN TRADITIONAL AND LOW LACTOSE YOGURT DURING FERMENTATION AND COOLING PERIODS

description: Abstract The purpose of this study was to evaluate the behavior of E. coli O157:H7 during lactose hydrolysis and fermentation of traditional and low lactose yogurt. It also aimed to verify E. coli O157:H7 survival after 12 h of storage at 4 ºC ±1 ºC. Two different types of yogurts were prepared, two with whole milk and two with pre-hydrolyzed whole milk; in both groups one yogurt was inoculated with E. coli O157:H7 and the other one was not inoculated. The survival of E. coli and pH of yogurt were determined during fermentation and after 12-h refrigeration. The results showed that E. coli O157:H7 was able to grow during the fermentation period (from 4.34 log CFU.mL-1 to 6.13 log CFU.mL-1 in traditional yogurt and 4.34 log CFU.mL-1 to 6.16 log CFU.mL-1 in low lactose yogurt). The samples with E. coli O157:H7 showed gas formation and syneresis. Thus, E. coli O157:H7 was able to survive and grow during fermentation of traditional and low lactose yogurts affecting the manufacture technology. Moreover, milk contamination by E. coli before LAB addition reduces the growth of L. bulgaricus and S. thermophilus especially when associated with reduction of lactose content.
DOI: 10.6084/m9.figshare.5671027.v1
title: Escherichia coli O157:H7 SURVIVAL IN TRADITIONAL AND LOW LACTOSE YOGURT DURING FERMENTATION AND COOLING PERIODS

description: Abstract The purpose of this study was to evaluate the behavior of E. coli O157:H7 during lactose hydrolysis and fermentation of traditional and low lactose yogurt. It also aimed to verify E. coli O157:H7 survival after 12 h of storage at 4 ºC ±1 ºC. Two different types of yogurts were prepared, two with whole milk and two with pre-hydrolyzed whole milk; in both groups one yogurt was inoculated with E. coli O157:H7 and the other one was not inoculated. The survival of E. coli and pH of yogurt were determined during fermentation and after 12-h refrigeration. The results showed that E. coli O157:H7 was able to grow during the fermentation period (from 4.34 log CFU.mL-1 to 6.13 log CFU.mL-1 in traditional yogurt and 4.34 log CFU.mL-1 to 6.16 log CFU.mL-1 in low lactose yogurt). The samples with E. coli O157:H7 showed gas formation and syneresis. Thus, E. coli O157:H7 was able to survive and grow during fermentation of traditional and low lactose yogurts affecting the manufacture technology. Moreover, milk contamination by E. coli before LAB addition reduces the growth of L. bulgaricus and S. thermophilus especially when associated with reduction of lactose content.