cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0v123z3
title: CIL:12106, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements
description: An image of the base of a dikinetid deeper in the cell. A postciliary ribbon and other microtubules fan out from it's basal bodies. TEM taken on 6/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned with a flatbed scanner to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2863) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0sb450m
title: CIL:122, Gallus gallus gallus, erythrocyte. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons; charge coupled device (CCD)
Parameter imaged: electron density
Source of contrast: elastic scattering
Visualization methods: uranyl salt; uranium compound; gold
Processing history: unprocessed raw image
description: Telomeres isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Sample was also labeled for the telomere protein TRF1 using immunogold.
Image recorded with FEI Tecnai 12 TEM at 100KV and recorded on 2048x2048 CCD with 2x binning.
Telomeres have a looped conformation, which likely aids in their protection from the rapid degradation that occurs with free DNA ends.
DOI: 10.6075/j0bz655s
title: CIL:12104, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements
description: This image shows a dikinetid which is set in a shallow basin in the cell surface. The pair of basal bodies have a proximal cartwheel and a dense septum (not found in most bbs) just distal to the cartwheel. The terminal plate separates the basal body from the cilium that arises from the distal end of the basal body. TEM taken on 5/31/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The negative was printed to paper and the image was scanned with a flatbed scanner and 0.5µm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2856) is in the library and available for quantitative analysis.Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0pr7tnb
title: CIL:12291, Mus musculus, epithelial cell. In Cell Image Library

description: Colocalization of the microtubule anchoring factor LL5α (red) with integrin α6 (blue) in the basal cortex of outer epithelium of mouse mammary gland tissue. LL5 did not colocalize with β-catenin-positive lateral membranes (green) of the lateral membrane. Overall findings from this publication showed that signaling from laminin-integrin associations play a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file from Fig. 4Ba, J Cell Biol (2010) 189 (5):901-917.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Cy2; X-Rhodamine; Cy5
Data qualification: Raw;spatialmeasurements
description: Mammary tissues from C57BL6/J strain mice (8 wk of age) were prepared by perfusion fixation, processed as described previously (Nakatani et al., 2004), and immunostained for β-catenin (Cy2, 488 nm laser), LL5 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system.
DOI: 10.6075/j0ww7gd9
title: CIL:12287, Homo sapiens, epithelial cell. In Cell Image Library

description: MCF-10Aeco cells expressing GFP-α-tubulin and RFP-LL5α were seeded on glass-bottomed dishes at a low density and cultured overnight. Time-lapse images of the cells were collected at 2.5-min intervals for 8 h 6 min using a DeltaVision Core Systemdriven by SoftWoRx software (Applied Precision) equipped with an IX70 microscope with a Plan-Apochromat 100× NA 1.40 oil immersion objective (Olympus), a cooled charge-coupled device camera (CoolSNAP HQ2; Photometrics) and a CO2 incubator (Tokai Hit Co., Ltd.). GFP, Ex, 470; Em, 525; RFP, Ex, 572, Em, 632.
description: Behaviors of the microtubule anchoring factor RFP-LL5α (red) and microtubules (green) in living epithelial cells undergoing random migration. During the time-lapse imaging, the cells divided into two daughter cells. Cortical patches of RFP-LL5α appear when cells become attached to the glass substrate, but disappear when the cells become detached at the onset of cell division. The patches reappear soon after the cells adhere to the glass again. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This is the original data file for Video 4 from J Cell Biol (2010) 189 (5):901-917.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed-monomerFP; EGFP
Data qualification: Raw
DOI: 10.6075/j0hd7tcr
title: CIL:12286, Mus musculus, fibroblast. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Fluorescein (FITC); Alexa Fluor 568; Alexa Fluor 633; primary antibody plus labeled secondary antibody
Processing history: color combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: This confocal image of wild type cells plated on cell-derived matrices that correspond to an accompanying image of beta3-/- integrin cells. There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-/- cells. The cells are co-stained for active beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals. Images correspond to Fig 1C in J Cell Bio. 2010. 189:369-383
DOI: 10.6075/j0vt1r8j
title: CIL:12301, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A High resolution image is available in the library (CIL:38861) for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements
description: Another view of the cytopharynx but in a dividing Didinium. This cytopharynx is in the proter (anterior) daughter cell. The lamellae, each consisting of a set of perpendicularly arranged microtubules cover this food-vacuole forming region. Vesicles lie near the cytopharyngeal membrane and fuse with the membrane. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. Bar = 1µm.
DOI: 10.60719/aphrc-2025-052
title: UNDERSTANDING THE LIVED EXPERIENCES OF PREGNANT AND PARENTING ADOLESCENTS IN BURKINA FASO, MALAWI AND KENYA

description: Pregnant and parenting adolescents (PPAs) in African countries face unique challenges that impact their well-being and life opportunities. Limited research exists on their lived experiences, including the impact of pregnancy on education and their overall well-being. Our studies aimed to document the lived experiences of PPAs, including the impact of pregnancy on their education, health, and aspirations.

We used mixed-methods cross-sectional approaches to conduct the studies in Burkina Faso, Kenya and Malawi. Data were collected from a total of 980, 594, and 669 PPAs in Burkina Faso, Kenya, and Malawi, respectively. These data are crucial for informing policy changes and interventions to better support pregnant and parenting adolescents. The data can also help shed light on the challenges faced by PPAs and the potential ways to improve their well-being and opportunities.
DOI: 10.6075/j04f1pg3
title: CIL:12295, Homo sapiens, epithelial cell. In Cell Image Library

description: Three dimensional data set acquired using a fast z-scan along the apical-to-basilar axis of a polarized epithelial cell in vitro to capture GFP-EB1 comets assosiated with the plus-end of actively polymerizing microtubules (green), and the microtubule anchoring factor RFP-LL5α (red). The apical region of the cell is at the bottom of the Z-axis, and basilar region is at the top. This is the original data file from Fig. 8 C from J Cell Biol (2010) 189 (5):901-917.
description: MCF-10Aeco cells expressing EB1-GFP and RFP-LL5 were cocultured with parental non-fluorescent cells on glass-bottomed dishes (fluorescent:non-fluorescent cells=1:10). Z-scanning images from the apical (bottom of the Z-axis here) to basal planes (top of the Z axis) of the cell were collected using a Revolution XD system driven by IQ software (Andor Technology) equipped with an IX81 inverted microscope with a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus), an electron-multiplying charge-coupled device cam- era (iXon DU-888; Andor Technology), a confocal spinning disk (CSU22; Yokogawa), a piezo-Z stage, a laser combiner with an acousto-optic tun- able filter and a CO2 incubator for live cell culture.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed-monomerFP; EGFP
Data qualification: Raw
DOI: 10.6075/j05m64fz
title: CIL:12289, Homo sapiens, epithelial cell. In Cell Image Library

description: MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies. LL5β (Cy5, 633 nm laser), laminin-5 (rhodamine red-X, 543 nm laser)) and integrin α6 (Cy2, 488 nm laser, and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917.
description: Preparation: methanol fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Cy2; Cy5; X-Rhodamine
Data qualification: Raw;spatialmeasurements
description: To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5β, MCF-10A epithelial cells were immunostained with antibodies to the secreted autocrine laminin-5 (green), the laminin receptor integrin α6 (blue) and LL5β (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-b from J Cell Biol (2010) 189 (5):901-917.
DOI: 10.6084/m9.figshare.30498416
title: Evaluation of Cardiac Biomarkers in Serum and Saliva of Heart Failure Patients

description: Heart failure (HF) is progressive, with high morbidity and mortality. Salivary biomarkers are being explored as non-invasive tools for HF monitoring. This study examined salivary NT-proBNP and hs-cTn alongside serum levels to distinguish HF patients from healthy controls and to assess diagnostic potential and cross-matrix concordance.
DOI: 10.6075/j0bp027d
title: CIL: 51679, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library

description: Imaging mode: DICM
Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective
description: ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C

Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files:
1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html)
2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/)
3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory
4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK"
5. The 16 bit image will typically look dark and will require brightness adjustment.
6. In the ImageJ toolbar, select Image &gt;&gt; Adjust &gt;&gt; Brightness/Contrast and select "Auto", or manually set the brightness and contrast.
description: A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding drug slowly in 1/4 increments over 90 minutes.
DOI: 10.6084/m9.figshare.30498416.v1
title: Evaluation of Cardiac Biomarkers in Serum and Saliva of Heart Failure Patients

description: Heart failure (HF) is progressive, with high morbidity and mortality. Salivary biomarkers are being explored as non-invasive tools for HF monitoring. This study examined salivary NT-proBNP and hs-cTn alongside serum levels to distinguish HF patients from healthy controls and to assess diagnostic potential and cross-matrix concordance.
DOI: 10.6084/m9.figshare.30498416.v2
title: Evaluation of Cardiac Biomarkers in Serum and Saliva of Heart Failure Patients

description: Heart failure (HF) is progressive, with high morbidity and mortality. Salivary biomarkers are being explored as non-invasive tools for HF monitoring. This study examined salivary NT-proBNP and hs-cTn alongside serum levels to distinguish HF patients from healthy controls and to assess diagnostic potential and cross-matrix concordance.
DOI: 10.6075/j0ng4ps8
title: CIL:12109, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements
description: An image of a dikinetid in cross section near its proximal end. A postciliary ribbon and a transverse ribbon are seen. A short kinetodesmal fiber buds from the side of the kinetid. Coated vesicles or pits are located nearby that are part of the endosomal system. TEM taken on 6/3/69 by R. Allen with Philips 300 operating at 60kV. Bar = 0.5µm. The negative was printed to paper and the image was scanned with a flatbed scanner and 0.5µm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2861) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0ms3rx9
title: CIL:12108, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A cross section showing only one basal body in two different dikinetids. Cross sections of overlapping microtubular ribbons arising from the dikinetids are seen in the ridges next to the basal body. Parasomal sacs can penetrate the pellicle on both sides of the dikinetids. TEM taken on 6/3/69 by R. Allen with Philips 300 TEM operating at 60kV. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned with a flatbed scanner to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2859) is in the library and available for quantitative analysis.Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0154g51
title: CIL:12111, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements
description: A micrograph showing the partially open cytoproct of Colpoda cucullus. The alveoli stop at the edges of the cytoproct and filamentous material (actin?) coats the inside of the single membrane that covers the open cytoproct. TEM taken on 5/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2858) is in the library and available for quantitative analysis.Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0hm57qh
title: CIL:1989, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.