DOI: 10.22002/b54n4-37125
title: Caulobacter crescentus

description: CB15N strain 1208 CreS deletion. 5 of 5, CreS deletion + A22, 2 memory errors during collection, data collected in 3 parts then merged using Priism. 2 contstrictions of very extended division-deficient cell; cytoplasmic filaments in lower constriction; ribosome-free area in upper constrictionfilament segmented by Sarah
description: <strong>Tilt Series Date:</strong> 2006-04-06</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 0.7°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep: Ariane Briegel; data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; exponential phase; A22 added to depolymerize MreB</p>
<strong>Sample Preparation:</strong> box 519, grid 1; see Ariane Briegel's notes</p>
  pdd2006-04-06-5_slicer4697.jpg: Nucleoid / REZ pdd2006-04-06-5_slicer4700.jpg: Unknown pdd2006-04-06-5_slicer4704.jpg: Unknown pdd2006-04-06-5_slicer4707.jpg: Unknown tube pdd2006-04-06-5_slicer4708.jpg: Unknown tube
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/rawdata/</p>cc1208_06Apr06e150t05.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/rawdata/cc1208_06Apr06e150t05.mrc"
        > <i class="download icon"></i> Download </a></p>

        cc1208_06Apr06e150t05b.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/rawdata/cc1208_06Apr06e150t05b.mrc"
        > <i class="download icon"></i> Download </a></p>

        cc1208_06Apr06e150t05c.mrc,  Tilt Series (Pixel Size 1.2 nm), 729.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/rawdata/cc1208_06Apr06e150t05c.mrc"
        > <i class="download icon"></i> Download </a></p>

        06Apr06t05bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/3dimage_300/06Apr06t05bn_full.rec"
        > <i class="download icon"></i> Download </a></p>

        pd2006-04-06-5_clip01.rec,  Reconstruction (Pixel Size 2.4 nm) cropped by Sarah, 204.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/3dimage_13104/pd2006-04-06-5_clip01.rec"
        > <i class="download icon"></i> Download </a></p>

        pd2006-04-06-5_clip01-label-Filament01.am,  filament segmented by Sarah, 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-5/file_3019/pd2006-04-06-5_clip01-label-Filament01.am"
        > <i class="download icon"></i> Download </a></p>