DOI: 10.5061/dryad.qz612jmv2
title: Replicated differential expression analysis in a green-brown polymorphic grasshopper reveals role of beta-carotene-binding protein in body coloration

description: This dataset accompanies a study on the genetic basis of green–brown color
 polymorphism in the club-legged grasshopper (Gomphocerus sibiricus). It
 includes RNA-seq data from fourth-instar nymphs (N4) of green and brown
 morphs, along with associated metadata (e.g., morph, sex) and analysis
 scripts. Transcriptomes were sequenced to investigate differential gene
 expression linked to coloration, and a reference-guided transcriptome
 assembly was generated. Six genes consistently upregulated in green
 individuals across two datasets were identified as beta-carotene-binding
 proteins (βCBPs), suggesting a role in carotenoid-mediated pigmentation.
 This dataset enables further functional and evolutionary analyses of βCBP
 genes in Orthoptera and may be reused in studies of gene expression,
 pigmentation, and evolutionary developmental biology.
description: # Replicated differential expression analysis in a green-brown polymorphic
 grasshopper reveals role of beta-carotene-binding protein in body
 coloration Dataset DOI: [10.5061/dryad.qz612jmv2](10.5061/dryad.qz612jmv2)
 ## Description of the data and file structure This dataset was generated
 as part of a study investigating the genetic basis of green–brown color
 polymorphism in the club-legged grasshopper (*Gomphocerus sibiricus*). We
 collected early-instar nymphs from the wild and reared them under
 laboratory conditions until the fourth instar (N4), when they were
 phenotyped and snap-frozen for RNA extraction. Transcriptome sequencing
 (RNA-seq) was performed on green and brown morphs to identify
 differentially expressed genes associated with coloration. The data
 include: * A reference-guided transcriptome assembly
 (`Gsib_color_transcriptome.fa`). * Metadata for two datasets: the
 discovery and confirmatory datasets (`meta.discovery_dataset.txt`,
 `meta.confirmatory_dataset.txt`). * A transcript-to-gene mapping file used
 in downstream gene-level expression analysis (`tx2gene.dta.tsv`). * The
 full analysis pipeline and commands used to process and analyze the data
 (`script.txt`). These files support reproducibility of the study and are
 intended for reuse in related research on pigmentation, gene expression,
 and evolutionary biology in Orthoptera. ### Files and variables #### File:
 Gsib_color_transcriptome.fa **Description:** The reference-guided
 transcriptome assembly #### File: tx2gene.dta.tsv **Description:** Mapping
 between transcript IDs and gene IDs #### File:
 meta.confirmatory_dataset.txt **Description:** meta information of the
 individuals in confirmatory dataset #### File: script.txt
 **Description:**  all commands used during the analysis #### File:
 meta.discovery_dataset.txt **Description:**  meta information of the
 individuals in discovery dataset ## Code/software The file script.txt
 contains all the commands we used during the analysis. ## Access
 information Other publicly accessible locations of the data: The raw reads
 have been submitted to NCBI, under BioProject PRJNA1241690