cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/2f2v2-rec29
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-05-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-12/3dimage_69221/</p>12may04_C126_3_pnlDN_Cos7_AT1BT2_0013_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-12/3dimage_69221/12may04_C126_3_pnlDN_Cos7_AT1BT2_0013_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may04_C126_3_pnlDN_Cos7_AT1BT2_0013.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-12/rawdata/12may04_C126_3_pnlDN_Cos7_AT1BT2_0013.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.3hzgpr
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Dromaius novaehollandiae",
 "Country is Poland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005845-250827131500795/datasets/export for details.
DOI: 10.22002/w8swy-c5r67
title: MJ007 fil-2

description: cells were exposed to a division inhibitor fil-2
description: <strong>Tilt Series Date:</strong> 2012-03-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Justin Nodwell</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in 82uM concentration of fil-2 inhibitor for 30min.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-5/rawdata/</p>fil20004.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-5/rawdata/fil20004.mrc"
        > <i class="download icon"></i> Download </a></p>

        fil20004_full.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-5/3dimage_17645/fil20004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/k03zt-6c726
title: TRIM5-21R delSpry

description: TRIM5-21R delSpry 2D lattice prepared by Barbie Ganser-Pornillos and  Viswanathan Chandrasekaran
description: <strong>Tilt Series Date:</strong> 2012-11-23</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Viswanathan Chandrasekarangrids purified the protein, Barbie Ganser-Pornillos assembled protein into 2D lattice. The sample was was plunge frozen and imaged at Caltech by Cora Woodward</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-23-12/rawdata/</p>12nov23_TRIM5_21R-delSpry_grid1_AT11BT1_0001.mrc,  Tilt Series (Pixel Size 0.45 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-23-12/rawdata/12nov23_TRIM5_21R-delSpry_grid1_AT11BT1_0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        12nov23_TRIM5_21R-delSpry_grid1_AT11BT1_0001_part122_3.rec,  Reconstruction (Pixel Size 1.8 nm), 353.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-23-12/3dimage_23861/12nov23_TRIM5_21R-delSpry_grid1_AT11BT1_0001_part122_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wwz10-8cp75
title: UU 2803- A413T delta P1P2

description: E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB medium, lysed with penicillin for 1 hour</p>
<strong>Sample Preparation:</strong> plunge frozen in R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-15/rawdata/</p>2803atlas10015.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-15/rawdata/2803atlas10015.mrc"
        > <i class="download icon"></i> Download </a></p>

        2803atlas10015_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-15/3dimage_20526/2803atlas10015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/04qcq-x7y62
title: Vibrio strain #1604

description: vc998 overexpression
description: <strong>Tilt Series Date:</strong> 2012-03-21</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> V52</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Matt Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown in LB at 30 degrees, induced with 0.1% arabinose for one hour</p>
  aba2012-03-21-1_slicer6013.jpg: Extracellular vesicle aba2012-03-21-1_slicer6014.jpg: Flat vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-21-1/rawdata/</p>Vibrio1604oe1.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-21-1/rawdata/Vibrio1604oe1.mrc"
        > <i class="download icon"></i> Download </a></p>

        Vibrio1604oe1_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-21-1/3dimage_17896/Vibrio1604oe1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.30006133
title: Porous Grain-Based Modeling of Coral Reef Limestone: Revealing Heterogeneity and Strain Rate Effects

description: Deep coral reef limestone (CRL) exhibits strong heterogeneity due to complex pore and crystal morphology, which significantly affect its strength and damage mechanisms. The scarcity of deep CRL samples limits a comprehensive understanding of its pore characteristics. The computed tomography scan and pore analysis for 112 samples and 3 m drilling cores of deep CRL indicate that the Pearson correlation coefficient of pore principal axes a and b reaches 0.928. The distribution of pore parameters was analyzed by kernel density estimation, and new pores consistent with the characteristics of deep CRL pores were generated by resampling the inverse cumulative distribution function. Subsequently, a porous grain-based modeling strategy was proposed, and the novel finite-discrete element method was adopted to study the effects of pore structure, crystal morphology, and strain rate on the mechanical parameters and macro-micro damage mechanisms of the CRL. The results indicate that the crack propagation under quasi-static and intermediate strain rates is affected by crystal arrangement and pore weak zones, while it is not affected under high strain rates. Strength increases with increasing crystal sphericity, and large-sized pores increase the tendency of sample volume dilatancy. Additionally, the pore size effect was revealed to ensure the mechanical test reliability of deep CRL. This study realized the refined modeling for deep CRL and solved the sample-dependent issue of reflecting CRL pore characteristics in numerical models.
DOI: 10.6084/m9.figshare.30006133.v1
title: Porous Grain-Based Modeling of Coral Reef Limestone: Revealing Heterogeneity and Strain Rate Effects

description: Deep coral reef limestone (CRL) exhibits strong heterogeneity due to complex pore and crystal morphology, which significantly affect its strength and damage mechanisms. The scarcity of deep CRL samples limits a comprehensive understanding of its pore characteristics. The computed tomography scan and pore analysis for 112 samples and 3 m drilling cores of deep CRL indicate that the Pearson correlation coefficient of pore principal axes a and b reaches 0.928. The distribution of pore parameters was analyzed by kernel density estimation, and new pores consistent with the characteristics of deep CRL pores were generated by resampling the inverse cumulative distribution function. Subsequently, a porous grain-based modeling strategy was proposed, and the novel finite-discrete element method was adopted to study the effects of pore structure, crystal morphology, and strain rate on the mechanical parameters and macro-micro damage mechanisms of the CRL. The results indicate that the crack propagation under quasi-static and intermediate strain rates is affected by crystal arrangement and pore weak zones, while it is not affected under high strain rates. Strength increases with increasing crystal sphericity, and large-sized pores increase the tendency of sample volume dilatancy. Additionally, the pore size effect was revealed to ensure the mechanical test reliability of deep CRL. This study realized the refined modeling for deep CRL and solved the sample-dependent issue of reflecting CRL pore characteristics in numerical models.
DOI: 10.22002/ajm42-3ez80
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2012-11-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 115.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-18/rawdata/</p>Bdellovibrio_wt_20121114_0010003.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-18/rawdata/Bdellovibrio_wt_20121114_0010003.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdellovibrio_wt_20121114_0010003_full.rec,  Reconstruction (Pixel Size 1.6 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-18/3dimage_23516/Bdellovibrio_wt_20121114_0010003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gw8jd-29w15
title: Agrobacterium tumefaciens

description: VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB + antibiotics</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-5/rawdata/</p>vg3101_20005.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-5/rawdata/vg3101_20005.mrc"
        > <i class="download icon"></i> Download </a></p>

        vg3101_20005_part111_2.rec,  Reconstruction (Pixel Size 0.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-5/3dimage_48955/vg3101_20005_part111_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v6fzg-ynx36
title: Legionella_JV9246

description: Legionella_JV9246: DotF-sfGFP fusion to the wild-type gene on the chromosome (thyA) and ΔdotO.
description: <strong>Tilt Series Date:</strong> 2019-05-28</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-4/rawdata/</p>JV9246_area1_tomo0002.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-4/rawdata/JV9246_area1_tomo0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9246_area1_tomo0002_part41_2.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-4/3dimage_103362/JV9246_area1_tomo0002_part41_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zsp8k-x1a74
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-02</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-02-20/rawdata/</p>Test_Pseudomonas_dfliF_69_fix.mrc,  Tilt Series (Pixel Size 0.37 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-02-20/rawdata/Test_Pseudomonas_dfliF_69_fix.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_69_fix_full.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-02-20/3dimage_97467/Test_Pseudomonas_dfliF_69_fix_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d2j0c-yqy38
title: MEF cells

description: Date of collection wrong in the tilt series ID. Should be Jan 3, 2019
description: <strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-42/rawdata/</p>TS16.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-42/rawdata/TS16.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS16_bin4_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-42/3dimage_101858/TS16_bin4_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/51f57-rg259
title: MEF cells - Septin2 knock down

description: Septin2 was knockdown using retroviral delivery of shRNA. Purpose - mitochondrial division in the absence of Septin2.
description: <strong>Tilt Series Date:</strong> 2019-01-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun-Shik from Chan lab helped design the shRNA against Septin2 and provided transduction protocol. Shrawan and Danielle Grotjahn created cell line. Danielle FIB-milled the cells and Shrawan performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were treated with 10µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-15/rawdata/</p>TS8.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-15/rawdata/TS8.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS8_bin4_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 677.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2019-01-05-15/3dimage_102029/TS8_bin4_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>