cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0n879xk
title: CIL:48102, Homo sapiens, mammary epithelial cell. In Cell Image Library

description: Cryopreserved human mammary epithelial cells were fixed and stained for cytokeratin 18, E-cadherin (from BD Transduction), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
description: Cryopreserved human mammary epithelial cells were revived and stained for cytokeratin 18 (in red) to reveal the keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue, E-cadherin (in green) to visualize the calcium-dependent cell-cell adhesion glycoprotein, and DAPI (in blue) to label nuclei.
description: Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of epitope
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Data qualification: Processed
DOI: 10.6075/j0hh6k7q
title: CIL:48101, Homo sapiens, hair follicle dermal papilla cell. In Cell Image Library

description: Human hair follicle dermal papilla cells were fixedand immunostained for vimentin (monoclonal V9 from Sigma), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
description: Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
description: Cryopreserved human hair follicle dermal papilla cells were revived and stained for vimentin (in green) to reveal cytoskeleton, and DAPI (in blue) to label nuclei.
DOI: 10.6075/j0833s69
title: CIL:48061, Cricetulus griseus, Ovary cells, CHO cells. In Cell Image Library

description: A population of CHO cells constitutively overexpressing a clone of human IgG2/kappa. CHO cells are regarded as the workhorse of recombinant protein expression and biopharmaceutical production. Under certain conditions such as when protein synthesis and protein folding efficiency exceed the capacity of ER-to-Golgi transport, export ready IgG species can progressively accumulate in the ER until a phase separation threshold of the protein is reached to crystallize. This human IgG2/kappa clone induced rod-shaped crystalline body in the ER of engineered CHO cells. Protein crystallization events physically prevented cytokinesis, without hampering nuclear division. The net result was the multi-nucleation and marked cell enlargement. The size of crystal-laden cells became markedly larger than the cells that do not house crystals.
DOI: 10.6075/j0dr2vnc
title: CIL:48015, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite. This medium spiny neuron was contained in a thick section from the neostriatum of a dopamine transporter knock out mouse. The reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Visualization methods: intracellular filling
Data qualification: Processed
description: Micer were C57BL/129SvJ. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi 3MeV UHVEM. Single tilt images spanned -70 to 70° in 2° increments.
DOI: 10.6075/j0p84c1x
title: CIL:48013, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite. This medium spiny neuron was contained in a thick section from the neostriatum of a dopamine transporter knock out mouse. The reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Visualization methods: intracellular filling
Data qualification: Processed
description: Micer were C57BL/129SvJ. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi 3MeV UHVEM. Single tilt images spanned -70 to 70° in 2° increments.
DOI: 10.6075/j0t153sp
title: CIL:48012, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Visualization methods: intracellular filling
Data qualification: Processed
description: Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite. This medium spiny neuron was contained in a thick section from the neostriatum of a dopamine transporter knock out mouse. The reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
description: Micer were C57BL/129SvJ. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi 3MeV UHVEM. Single tilt images spanned -70 to 70° in 2° increments.
DOI: 10.6075/j0gm87fr
title: CIL:48108, Rattus norvegicus, Neural stem cells. In Cell Image Library

description: Relation to intact cell: dispersed cells in vitro
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Data qualification: Processed
description: Differentiated rat neural stem cells were fixed andimmunostained β III-tubulin (monoclonal Tuj1 from Stem Cell Technologies), GFAP (from Stem Cell Technologies) and DAPI (from Life Technologies). Images were acquired with SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
description: Differentiated rat neural stem cells stained for Œ≤ III-Tubulin (in green) to reveal neurons, GFAP (in red) to reveal glial cells, and DAPI (in blue) to label nuclei.
DOI: 10.6075/j0r211jx
title: CIL:48107, Rattus norvegicus, neural stem cell. In Cell Image Library

description: Differentiated rat neural stem cells stained for Œ≤ III-Tubulin (in blue) to reveal neurons and GFAP (in red) to reveal glial cells.
description: Differentiated rat neural stem cells were fixed andimmunostained β III-tubulin (monoclonal Tuj1 from Stem Cell Technologies) and GFAP (from Stem Cell Technologies). Images were acquired with SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
description: Relation to intact cell: dispersed cells in vitro
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Data qualification: Processed
DOI: 10.6075/j0q240dj
title: CIL:48302, Sprague-Dawley rat. In Cell Image Library

description: Images are acquired from fixed and living heart tissue using fiber-optics and laser-scanning confocal microscopy, respectively. Three sets of images denoted as CCM, FCMtopical, FCMcarrier were stored for subsequent analyses.
description: Preparation: chemically fixed tissue
Imaging mode: confocal microscopy
description: CCM images were from fixed rodent tissue preparations acquired using established methods (doi: 10.1161/circimaging.112.000121). In short, rodent hearts were isolated and Langendorff-perfused with 4% paraformaldehyde. Tissue from the atrial working myocardium, sinoatrial node, and atrioventricular node were dissected from the hearts and fluorescently labeled with wheat germ agglutinin conjugated to CF488A (29022-1; Biotium, Hayward, CA; 1:25). Fluorescently labeled tissue preparations were imaged using a conventional laser-scanning confocal microscope (Zeiss LSM5 Duo; Zeiss, Jena, Germany) with a 40x oil immersion lens (NA 1.3). The fluorescent signal was captured using an Argon/2 ion laser (488 nm excitation) and a bandpass filter (505 to 555 nm emission). Three-dimensional image stacks were acquired at a spatial resolution (xyz dimensions), field of view (xy) and a z-scan range of 0.2x0.2x0.2 μm, 204.8x204.8 μm and 50 μm, respectively. Example cross-sections through the image stacks were stored as CCM images.
DOI: 10.6075/j0m908vn
title: CIL:48105, Rattus norvegicus, astrocyte. In Cell Image Library

description: Rat astrocytes were fixed and immunostained for GFAP (from Stem Cell Technologies), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
description: Cryopreserved rat astrocytes were revived and stained for GFAP (in red) to reveal cytoskeleton and DAPI (in blue) to label nuclei. GFAP mutations and intracellular accumulation have been proposed to play an important role in a rare genetic disorder, Alexander Disease.
description: Relation to intact cell: Dispersed cells cryopreserved; revived and cultured in vitro
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of epitope
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
DOI: 10.6075/j0cv4hwv
title: CIL:48060, Cricetulus griseus, Ovary cells, CHO cells. In Cell Image Library

description: A population of CHO cells constitutively overexpressing a clone of human IgG2/kappa. CHO cells are regarded as the workhorse of recombinant protein expression and biopharmaceutical production. Under certain conditions such as when protein synthesis and protein folding efficiency exceed the capacity of ER-to-Golgi transport, export ready IgG species can progressively accumulate in the ER until a phase separation threshold of the protein is reached to crystallize. This human IgG2/kappa clone induced rod-shaped crystalline body in the ER of engineered CHO cells. Protein crystallization events physically prevented cytokinesis, without hampering nuclear division. The net result was the multi-nucleation and marked cell enlargement. The size of crystal-laden cells became markedly larger than the cells that do not house crystals.
DOI: 10.6075/j0668dc9
title: CIL:48009, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Micer were C57BL/129SvJ. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi 3MeV UHVEM. Single tilt images spanned -70 to 70° in 2° increments.
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Visualization methods: intracellular filling
Data qualification: Processed
description: Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite. This medium spiny neuron was contained in a thick section from the neostriatum of a dopamine transporter knock out mouse. The reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j0f76b43
title: CIL:48008, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library

description: Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7 - 11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Preparation: formaldehyde fixed tissue; vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements
description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j0f47mqc
title: CIL:48004, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library

description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7 - 11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Preparation: formaldehyde fixed tissue; vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements
DOI: 10.15468/dl.6dmy7f
title: Occurrence Download

description: A dataset containing 385399 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Geranium sylvaticum"
 ]
}

 The dataset includes 385399 records from 946 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009606-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.eyq5n6
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Scarites sulcatus Olivier, 1795",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 14 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0192563-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gdqb4y
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Peperomia argyreia (Miq.) É.Morren",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198475-220831081235567/datasets/export for details.
DOI: 10.15468/dl.zszhsp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pandorea jasminoides (Lindl.) Schumann",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0192566-220831081235567/datasets/export for details.
DOI: 10.15468/dl.zd6uw9
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Argemone grandiflora Sweet",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198478-220831081235567/datasets/export for details.
DOI: 10.15468/dl.wut7x5
title: Occurrence Download

description: A dataset listing the 2861 species recorded in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Specimen, Human Observation)",
 "Country is Mexico",
 "Elevation 0.0-1502.0m",
 "Geometry POLYGON((-92.5169 13.32221,-86.87941 18.40414,-85.1177 22.36645,-91.34242 22.14906,-94.7484 19.95707,-96.04033 20.61804,-97.2148 22.25779,-97.09736 23.6636,-110.25149 26.74478,-106.37572 21.60411,-107.19785 20.17771,-95.68799 14.68965,-94.39606 15.59659,-92.5169 13.32221))",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Aves"
 ]
}

 The dataset's 2861 records were derived from 101 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0229780-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c2ymf3
title: Occurrence Download

description: A dataset listing the 40809 species recorded in GBIF matching the query:
{
 "and" : [
 "Country is Thailand",
 "TaxonKey is Animalia"
 ]
}

 The dataset's 40809 records were derived from 1409 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198470-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4esx9r
title: Occurrence Download

description: A dataset containing 543 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rheum palmatum L."
 ]
}

 The dataset includes 543 records from 74 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0247528-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z6dz2y
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Scandentia",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0256560-220831081235567/datasets/export for details.
DOI: 10.15468/dl.mpx98c
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Peperomia metallica L.Linden & Rodigas",
 "HasCoordinate is true",
 "Country is Japan",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0198476-220831081235567/datasets/export for details.
DOI: 10.15468/dl.9uuqtr
title: Occurrence Download

description: A dataset listing the 75046 species recorded in GBIF matching the query:
{
 "and" : [
 "Country is Thailand",
 "BasisOfRecord is one of (Human Observation, Observation, Occurrence evidence, Machine Observation, Material sample, Specimen)"
 ]
}

 The dataset's 75046 records were derived from 876 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0244867-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.