cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0cz36hv
title: CIL:40277, Helianthus annuus, pollen. In Cell Image Library

description: Scanning electron micrograph of Helianthus annuus (Sunflower) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j0xk8dwb
title: CIL:40287, Allium, pollen. In Cell Image Library

description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Allium pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
DOI: 10.6075/j0zs2vtx
title: CIL:40286, Pinaceae, pollen. In Cell Image Library

description: Scanning electron micrograph of Pinaceae strobus (Eastern white pine) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
DOI: 10.6075/j0765dph
title: CIL:40285, Betula papyrifera, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Scanning electron micrograph of Betula papyrifera (Paper Birsh) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
DOI: 10.6075/j0z31z04
title: CIL:40284, Ricinus communis, pollen. In Cell Image Library

description: Scanning electron micrograph of Ricinus communis (Castor bean plant) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j0pz5849
title: CIL:40297, Lilium longiflorum, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Lilium longiflorum (Trumpet Lily) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j0q81cd1
title: CIL:40296, Rosa rugosa, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Rosa rugosa (Beach rose) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j00k27wm
title: CIL:40303, Nicotiana alata, pollen. In Cell Image Library

description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Scanning electron micrograph of Nicotiana alata (tobacco) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
DOI: 10.6075/j0qc02tb
title: CIL:40295, Ambrosia artemisiifolia, pollen. In Cell Image Library

description: Scanning electron micrograph of Ambrosia artemisiifolia (ragweed) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
DOI: 10.6075/j0057f8s
title: CIL:40291, Pinaceae, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Pinaceae strobus (Eastern white pine) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j0862ftw
title: CIL: 50696, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library

description: Imaging mode: Serial electron microscopy; focused ion beam scanning electron mictoscopy (FIB-SEM)
Visualization methods: Heavy metal stain (osmium tetroxide, potassium ferrocyanide, tannic acid), immunogold labeling using 10 nm gold beads
description: Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 
2.8_Cell_2_MATLAB_Volume_Analysis_Results is a compressed (zipped) folder containing all files required to execute the MATLAB volume analysis. The folder also includes the resulting output files and figures.
This file is a part of Dataset 2, comprised of files related to the Cell #2 of current study. The numbering order of the files reflects the order of operations performed during the analysis.
description: FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
DOI: 10.6075/j0p55mvx
title: CIL:40289, Amaranthus, pollen. In Cell Image Library

description: Scanning electron micrograph of Amaranthus opopeo pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
DOI: 10.6075/j0ww7h1s
title: CIL: 50692, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 2.5_Cell_2_Stereocilia_Segmentation. In Cell Image Library

description: Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 
2.5_Cell_2_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results (1.95×1.95×10 nm³ voxel size). Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview.
This file is a part of Dataset 2, comprised of files related to the Cell #2 of current study. The numbering order of the files reflects the order of operations performed during the analysis.
description: FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
description: Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Mictoscopy (FIB-SEM)
Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads
DOI: 10.6075/j08k77zw
title: CIL:40313, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: wide-field detection; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 594; EGFP
Data qualification: Processed
description: HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Tpr and observed with a Delta-vision deconvolution system. 
See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827.
description: Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and Tpr, a nucleoporin that localizes to the nucleoplasmic filaments of nuclear pore complexes(red), within the nuclear envelope of HeLa cells. There is no overlap between Tpr and ALADIN.
 Other images in this group show the ultrastructural effects of mutant ALADIN.
DOI: 10.6075/j0251h28
title: CIL:40305, Saccharomyces cerevisiae, BY4716. In Cell Image Library

description: Transmission electron micrograph of a yeast cell exposed to 60¬µM a sertraline. This high magnification image from CIL 39708 shows cisternal structures and a lipid droplet. The image contributor asks Library users to post comments answering the following questions: Is the elongated cisternal organelle ER?
description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.
DOI: 10.6075/j0st7p51
title: CIL:40300, Petunia, pollen. In Cell Image Library

description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Petunia pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
DOI: 10.6075/j0p84b6r
title: CIL:40299, Nicotiana alata, pollen. In Cell Image Library

description: Complete specimen preparation protocol available at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
description: Scanning electron micrograph of Nicotiana alata (tobacco) pollen. These specimens have been acetolyzed to remove cytoplasm and pollenkit in order to reveal the intricate wall structure. This is part of a large image collection of scanning electron micrographs of pollen samples. Images of a particular species are grouped together. However, to find images and groups of images from other species, please search for the the name of the contributor, Louisa Howard.
description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold
Processing history: unprocessed raw data
DOI: 10.6075/j05d8r7d
title: CIL: 50690, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library

description: Imaging mode: Serial electron microscopy; focused ion beam scanning electron mictoscopy (FIB-SEM)
Visualization methods: Heavy metal stain (osmium tetroxide, potassium ferrocyanide, tannic acid), immunogold labeling using 10 nm gold beads
description: FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
description: Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 
2.3_Cell_2_Stack_Aligned is a multipage TIFF file containing aligned images of Cell#2 (1.95×1.95×10 nm³ voxel size).
This file is a part of Dataset 2, comprised of files related to the Cell #2 of current study. The numbering order of the files reflects the order of operations performed during the analysis.
DOI: 10.6075/j0pz57kq
title: CIL:40267, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library

description: Time lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at prophase in a vertical strip perpendicular to the spindle orientation. Microtubules in the activated strip show a variety of motions both toward and away from spindle poles. This contrasts with the exclusively poleward motion of spindle microtubules seen at metaphase (and illustrated in the grouped movie CIL:40266). The time series of images used to prpeare this movie is included in the group of images.
 See Fig 1A in: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002
description: Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp.
See also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed
DOI: 10.6075/j0kw5g5m
title: CIL:40264, Rattus norvegicus, astrocyte of the hippocampus. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Visualization methods: intracellular filling; lucifer yellow
Data qualification: Processed
description: Tissue was sectioned at a thickness of 100 µm with vibratome. Optical sections (at intervals of 0.2 µm) were gathered using a Biorad Radiance 2000 Confocal, 60X N.A. 1.4 oil-immersion objective.
description: Maximum intensity projection of an optical section series of a protoplasmic astrocyte injected with Lucifer Yellow from the hippocampal area CA1 in a 1 week old male rat. For more information, see: Bushong et al. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j0mc8xsb
title: CIL:40270, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library

description: Living LLCPK1 cells expressing GFP-alpha-tubulin were observed using a Nikon Eclipse TE 300 microscope equipped with a 100x phase NA 1.4 objective lens, and images captured at 2 sec intervals with a Perkin Elmer spinning disc confocal scan head and a cooled CCD camera
See also supplemental video 1 in: NM Rusan et al. 2002 Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport. J Cell Biol 158:997-1003.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed
description: Time lapse movie showing the change in microtubule distribution in cultured porcine kidney epithelial cells (LLCPK1) stably expressing GFP-alpha tubulin during the progression from prophase to prometaphase. The cytoskeleton is reorganized with disassembly of individual microtubules and formation of microtubule bundles, causing an inward collapse of the microtubule array prior to the formation of the mitotic spindle.