cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5718637
title: Characterization of air-liquid interface culture of A549 alveolar epithelial cells

description: Alveolar epithelia play an essential role in maintaining the integrity and homeostasis of lungs, in which alveolar epithelial type II cells (AECII) are a cell type with stem cell potential for epithelial injury repair and regeneration. However, mechanisms behind the physiological and pathological roles of alveolar epithelia in human lungs remain largely unknown, partially owing to the difficulty of isolation and culture of primary human AECII cells. In the present study, we aimed to characterize alveolar epithelia generated from A549 lung adenocarcinoma cells that were cultured in an air-liquid interface (ALI) state. Morphological analysis demonstrated that A549 cells could reconstitute epithelial layers in ALI cultures as evaluated by histochemistry staining and electronic microscopy. Immunofluorescent staining further revealed an expression of alveolar epithelial type I cell (AECI) markers aquaporin-5 protein (AQP-5), and AECII cell marker surfactant protein C (SPC) in subpopulations of ALI cultured cells. Importantly, molecular analysis further revealed the expression of AQP-5, SPC, thyroid transcription factor-1, zonula occludens-1 and Mucin 5B in A549 ALI cultures as determined by both immunoblotting and quantitative RT-PCR assay. These results suggest that the ALI culture of A549 cells can partially mimic the property of alveolar epithelia, which may be a feasible and alternative model for investigating roles and mechanisms of alveolar epithelia in vitro.
DOI: 10.6084/m9.figshare.5718637.v1
title: Characterization of air-liquid interface culture of A549 alveolar epithelial cells

description: Alveolar epithelia play an essential role in maintaining the integrity and homeostasis of lungs, in which alveolar epithelial type II cells (AECII) are a cell type with stem cell potential for epithelial injury repair and regeneration. However, mechanisms behind the physiological and pathological roles of alveolar epithelia in human lungs remain largely unknown, partially owing to the difficulty of isolation and culture of primary human AECII cells. In the present study, we aimed to characterize alveolar epithelia generated from A549 lung adenocarcinoma cells that were cultured in an air-liquid interface (ALI) state. Morphological analysis demonstrated that A549 cells could reconstitute epithelial layers in ALI cultures as evaluated by histochemistry staining and electronic microscopy. Immunofluorescent staining further revealed an expression of alveolar epithelial type I cell (AECI) markers aquaporin-5 protein (AQP-5), and AECII cell marker surfactant protein C (SPC) in subpopulations of ALI cultured cells. Importantly, molecular analysis further revealed the expression of AQP-5, SPC, thyroid transcription factor-1, zonula occludens-1 and Mucin 5B in A549 ALI cultures as determined by both immunoblotting and quantitative RT-PCR assay. These results suggest that the ALI culture of A549 cells can partially mimic the property of alveolar epithelia, which may be a feasible and alternative model for investigating roles and mechanisms of alveolar epithelia in vitro.
DOI: 10.6084/m9.figshare.5718634
title: Tadalafil protector effect during ischemia-reperfusion in rats

description: Abstract Purpose: To evaluate histological parameters in rat renal tissue after tadalafil use during hot ischemia for 45 minutes and reperfusion for 24 hours. Methods: Twenty rats were divided into 2 groups. In the experimental group 10 mg/kg of tadalafil was used per gavage before the procedure. All cases underwent left partial nephrectomy, followed by 45 minutes of warm ischemia. Left nephrectomy of the remaining kidney was performed after 24 hours from the initial procedure. The histological parameters analyzed were: detachment of tubular cells, accumulation of desquamated cells in the proximal tubule, loss of brush border, tubular cylinders, interstitial edema, leukocyte infiltration, capillary congestion, vacuolization, tubular dilatation, necrosis and collapse of the capillary tuft. Results: Two rats from each group died and were excluded from the study. Tadalafil significantly reduced leukocyte infiltration (p = 0.036). The remaining histological parameters did not show statistical difference between the groups. Conclusion: The use of tadalafil during warm ischemia and reperfusion demonstrates statistically significant reduction of leukocyte infiltration in the renal interstitium.
DOI: 10.6084/m9.figshare.5718631
title: Trends in the Prevalence of Live Macrosomic Newborns According to Gestational Age Strata, in Brazil, 2001-2010, and 2012-2014

description: Abstract Purpose To describe the trends in the prevalence of macrosomia (birth weight ± 4,000 g) according to gestational age in Brazil in the periods of 2001-2010 and 2012-2014. Methods Ecological study with data from the Brazilian Live Birth Information System (SINASC, in the Portuguese acronym) regarding singleton live newborns born from 22 gestational weeks. The trends in Brazil as a whole and in each of its five regions were analyzed according to preterm (22-36 gestational weeks) and term (37-42 gestational weeks) strata. Annual Percent Changes (APCs) based on the Prais-Winsten method and their respective 95% confidence intervals (CIs) were used to verify statistically significant changes in 2001-2010. Results In Brazil, the prevalence of macrosomic births was of 5.3% (2001-2010) and 5.1% (2012-2014). The rates were systematically higher in the North and Northeast Regions both in the preterm and in term strata. In the preterm stratum, the North Region presented the highest variation in the prevalence of macrosomia (+137.5%) when comparing 2001 (0.8%) to 2010 (1.9%). In the term stratum, downward trends were observed in Brazil as a whole and in every region. The trends for 2012-2014 were more heterogeneous, with the prevalence systematically higher than that observed for 2001-2010. The APC in the preterm stratum (2001-2010) showed a statistically significant trend change in the North (APC: 15.4%; 95%CI: 0.6-32.3) and South (APC: 13.5%; 95%CI: 4.8-22.9) regions. In the term stratum, the change occurred only in the North region (APC:-1.5%; 95%CI: -2.5--0.5). Conclusion The prevalence of macrosomic births in Brazil was higher than 5.0%. Macrosomia has potentially negative health implications for both children and adults, and deserves close attention in the public health agenda in Brazil, as well as further support for investigation and intervention.
DOI: 10.6084/m9.figshare.5718634.v1
title: Tadalafil protector effect during ischemia-reperfusion in rats

description: Abstract Purpose: To evaluate histological parameters in rat renal tissue after tadalafil use during hot ischemia for 45 minutes and reperfusion for 24 hours. Methods: Twenty rats were divided into 2 groups. In the experimental group 10 mg/kg of tadalafil was used per gavage before the procedure. All cases underwent left partial nephrectomy, followed by 45 minutes of warm ischemia. Left nephrectomy of the remaining kidney was performed after 24 hours from the initial procedure. The histological parameters analyzed were: detachment of tubular cells, accumulation of desquamated cells in the proximal tubule, loss of brush border, tubular cylinders, interstitial edema, leukocyte infiltration, capillary congestion, vacuolization, tubular dilatation, necrosis and collapse of the capillary tuft. Results: Two rats from each group died and were excluded from the study. Tadalafil significantly reduced leukocyte infiltration (p = 0.036). The remaining histological parameters did not show statistical difference between the groups. Conclusion: The use of tadalafil during warm ischemia and reperfusion demonstrates statistically significant reduction of leukocyte infiltration in the renal interstitium.
DOI: 10.6084/m9.figshare.5718631.v1
title: Trends in the Prevalence of Live Macrosomic Newborns According to Gestational Age Strata, in Brazil, 2001-2010, and 2012-2014

description: Abstract Purpose To describe the trends in the prevalence of macrosomia (birth weight ± 4,000 g) according to gestational age in Brazil in the periods of 2001-2010 and 2012-2014. Methods Ecological study with data from the Brazilian Live Birth Information System (SINASC, in the Portuguese acronym) regarding singleton live newborns born from 22 gestational weeks. The trends in Brazil as a whole and in each of its five regions were analyzed according to preterm (22-36 gestational weeks) and term (37-42 gestational weeks) strata. Annual Percent Changes (APCs) based on the Prais-Winsten method and their respective 95% confidence intervals (CIs) were used to verify statistically significant changes in 2001-2010. Results In Brazil, the prevalence of macrosomic births was of 5.3% (2001-2010) and 5.1% (2012-2014). The rates were systematically higher in the North and Northeast Regions both in the preterm and in term strata. In the preterm stratum, the North Region presented the highest variation in the prevalence of macrosomia (+137.5%) when comparing 2001 (0.8%) to 2010 (1.9%). In the term stratum, downward trends were observed in Brazil as a whole and in every region. The trends for 2012-2014 were more heterogeneous, with the prevalence systematically higher than that observed for 2001-2010. The APC in the preterm stratum (2001-2010) showed a statistically significant trend change in the North (APC: 15.4%; 95%CI: 0.6-32.3) and South (APC: 13.5%; 95%CI: 4.8-22.9) regions. In the term stratum, the change occurred only in the North region (APC:-1.5%; 95%CI: -2.5--0.5). Conclusion The prevalence of macrosomic births in Brazil was higher than 5.0%. Macrosomia has potentially negative health implications for both children and adults, and deserves close attention in the public health agenda in Brazil, as well as further support for investigation and intervention.
DOI: 10.6084/m9.figshare.5718625
title: TERRITORIALITY AND CULTURE AMONG THE KALUNGA: beyond the culturalism

description: This article presents a discussion about the identity resignifications that occurred in a remaining Quilombo community called Kalunga, located in northeast of the state of Goiás, Brazil, ranging from its formation and from the recognition of these communities by the Brazilian Federal Constitution of 1988. The recognition adds new identity problems that appear in the way in which the group experiences and organizes the territory. Considering an ethnographic study, I try to demonstrate that the ethnicity of the group is based on its own social and territorial organization, and that it is not possible to identify an object of limited contours – neither color, “race” or African culture, nor Quilombo origin – in which the individuals face each other and from which they inaugurate their identity.
DOI: 10.6084/m9.figshare.5718625.v1
title: TERRITORIALITY AND CULTURE AMONG THE KALUNGA: beyond the culturalism

description: This article presents a discussion about the identity resignifications that occurred in a remaining Quilombo community called Kalunga, located in northeast of the state of Goiás, Brazil, ranging from its formation and from the recognition of these communities by the Brazilian Federal Constitution of 1988. The recognition adds new identity problems that appear in the way in which the group experiences and organizes the territory. Considering an ethnographic study, I try to demonstrate that the ethnicity of the group is based on its own social and territorial organization, and that it is not possible to identify an object of limited contours – neither color, “race” or African culture, nor Quilombo origin – in which the individuals face each other and from which they inaugurate their identity.
DOI: 10.6084/m9.figshare.5718622
title: In vivo OCT assessment of anterior segment central axial lengths with accommodation

description: ABSTRACT Purpose: To assess changes in anatomic structures in the anterior eye segment in terms of axial lengths with accommodation via optical coherence tomography. Methods: In this observational study, 25 eyes of 25 healthy adults were examined using the Visante® omni optical coherence tomography system. Central corneal thickness, anterior chamber depth, central lens thickness, and anterior segment length were assessed. The evaluated parameters were obtained with accommodation using different stimulus vergences, namely 0.0, -1.0, -2.0, and -3.0 D. Variation of these parameters was compared among different levels of accommodation. Results: Central corneal thickness was not altered at any stimulus vergence during accommodation (p&gt;0.05). Conversely, anterior chamber depth was significantly reduced (p&lt;0.05), whereas central lens thickness was significantly increased (p&lt;0.05). Anterior segment length also increased with accommodation (p&lt;0.05), indicating backward movement of the posterior pole. Conclusions: There are significant variations in anterior segment lengths that occur with accommodation. Studying these changes will provide useful information regarding the accommodation mechanism that can improve our understanding of this process and facilitate clinical decision-making by practitioners.
DOI: 10.6084/m9.figshare.5718622.v1
title: In vivo OCT assessment of anterior segment central axial lengths with accommodation

description: ABSTRACT Purpose: To assess changes in anatomic structures in the anterior eye segment in terms of axial lengths with accommodation via optical coherence tomography. Methods: In this observational study, 25 eyes of 25 healthy adults were examined using the Visante® omni optical coherence tomography system. Central corneal thickness, anterior chamber depth, central lens thickness, and anterior segment length were assessed. The evaluated parameters were obtained with accommodation using different stimulus vergences, namely 0.0, -1.0, -2.0, and -3.0 D. Variation of these parameters was compared among different levels of accommodation. Results: Central corneal thickness was not altered at any stimulus vergence during accommodation (p&gt;0.05). Conversely, anterior chamber depth was significantly reduced (p&lt;0.05), whereas central lens thickness was significantly increased (p&lt;0.05). Anterior segment length also increased with accommodation (p&lt;0.05), indicating backward movement of the posterior pole. Conclusions: There are significant variations in anterior segment lengths that occur with accommodation. Studying these changes will provide useful information regarding the accommodation mechanism that can improve our understanding of this process and facilitate clinical decision-making by practitioners.
DOI: 10.6084/m9.figshare.5718619
title: FAPESP’s Innovative Small Business Program (PIPE) as development inducer of technology-based enterprises

description: Abstract This text examines the relevance of the development support for small business enterprises with a technological basis in the form of a “package” of strategic resources and capabilities, embedded in a short space of time through the FAPESP’s Innovative Small Business Program (PIPE). The study approach was the resource-based view (RBV). The qualitative methodology was adopted with the application of content analysis. The data were obtained during interviews based on a structured roadmap with open-ended questions conducted with the partners/managers of ten small technology-based business enterprises divided into two groups, one consisting of companies benefited by the PIPE and the other containing those that used their own resources. The intention was to compare their evolution. The study results indicated that the PIPE provided decisive support encouraging the development of the businesses benefiting from it. One of the relevant gains was the faster availability of additional resources as a result of the productive combination of the enterprises’ pre-existing resources and capabilities with those obtained through the program. An important finding was that small business enterprises with a technological basis have grown in the market, regardless of the help received, they featured differentiated technology, partners/managers with a clear view of their markets, and the aim of creating value for their products and services. This research limitations included the selection of small business enterprises with very different products to compare successes.
DOI: 10.6084/m9.figshare.5718619.v1
title: FAPESP’s Innovative Small Business Program (PIPE) as development inducer of technology-based enterprises

description: Abstract This text examines the relevance of the development support for small business enterprises with a technological basis in the form of a “package” of strategic resources and capabilities, embedded in a short space of time through the FAPESP’s Innovative Small Business Program (PIPE). The study approach was the resource-based view (RBV). The qualitative methodology was adopted with the application of content analysis. The data were obtained during interviews based on a structured roadmap with open-ended questions conducted with the partners/managers of ten small technology-based business enterprises divided into two groups, one consisting of companies benefited by the PIPE and the other containing those that used their own resources. The intention was to compare their evolution. The study results indicated that the PIPE provided decisive support encouraging the development of the businesses benefiting from it. One of the relevant gains was the faster availability of additional resources as a result of the productive combination of the enterprises’ pre-existing resources and capabilities with those obtained through the program. An important finding was that small business enterprises with a technological basis have grown in the market, regardless of the help received, they featured differentiated technology, partners/managers with a clear view of their markets, and the aim of creating value for their products and services. This research limitations included the selection of small business enterprises with very different products to compare successes.
DOI: 10.6084/m9.figshare.5718616
title: Effects of Gaba on the Thymus Cytokines of Wenchang Chickens Submitted to Heat Stress

description: ABSTRACT Under routine feeding conditions, 1-day-old male chicks were randomly divided into control group (CK), heat-stress group (HS), and GABA + heat stress group (GABA+HS). The thymus contents of IL-1, IL-2, TGF-β1, IFN-γ, GH and HSP70 were determined by enzyme-linked immunosorbent assays. The results showed that: (1) IL-1 and TGF-β1 contents of HS group were significantly lower than those of the CK group (p&lt;0.05), and those of the GABA+HS group were significantly higher relative to the HS group (p&lt;0.05); (2) IL-2 and IFN-γ contents of the HS group were significantly higher than those of the CK group (p&lt;0.05), and those of the GABA+HS group were significantly lower relative to the HS group (p&lt;0.05);(3) the thymus GH content of all three groups first increased and then decreased. The expression levels GH of the HS and GABA+HS groups were significantly lower than CK group (p&lt;0.05); and (4) HSP70 expression levels in the thymus were significantly higher in the HS and GABA+HS groups relative to the CK group (p&lt;0.05). These results indicate that heat stress affected thymus development, immune functions, and overall growth of chickens. Furthermore, it was shown that feeding GABA may significantly improve the immune responses of heat-stressed chickens by increasing the expression levels of IL-1 and TGF-β1, and effectively alleviate the negative effects of heat stress on thymus development by changing HSP70 expression and GH secretion.
DOI: 10.6084/m9.figshare.5718616.v1
title: Effects of Gaba on the Thymus Cytokines of Wenchang Chickens Submitted to Heat Stress

description: ABSTRACT Under routine feeding conditions, 1-day-old male chicks were randomly divided into control group (CK), heat-stress group (HS), and GABA + heat stress group (GABA+HS). The thymus contents of IL-1, IL-2, TGF-β1, IFN-γ, GH and HSP70 were determined by enzyme-linked immunosorbent assays. The results showed that: (1) IL-1 and TGF-β1 contents of HS group were significantly lower than those of the CK group (p&lt;0.05), and those of the GABA+HS group were significantly higher relative to the HS group (p&lt;0.05); (2) IL-2 and IFN-γ contents of the HS group were significantly higher than those of the CK group (p&lt;0.05), and those of the GABA+HS group were significantly lower relative to the HS group (p&lt;0.05);(3) the thymus GH content of all three groups first increased and then decreased. The expression levels GH of the HS and GABA+HS groups were significantly lower than CK group (p&lt;0.05); and (4) HSP70 expression levels in the thymus were significantly higher in the HS and GABA+HS groups relative to the CK group (p&lt;0.05). These results indicate that heat stress affected thymus development, immune functions, and overall growth of chickens. Furthermore, it was shown that feeding GABA may significantly improve the immune responses of heat-stressed chickens by increasing the expression levels of IL-1 and TGF-β1, and effectively alleviate the negative effects of heat stress on thymus development by changing HSP70 expression and GH secretion.
DOI: 10.6084/m9.figshare.5718613
title: Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly

description: Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer at –80°C for assessment of E-cadherin 1 (CDH1), SLUG (SNAI2), and NCAM (NCAM1) by real-time PCR. The samples were fixed in formalin and embedded in paraffin for immunohistochemical analysis of E-cadherin and NCAM. Thirty-five patients with acromegaly were included in the study. Of these, 65.7% had invasive tumors. Immunohistochemically, E-cadherin was expressed in 96.7% of patients, and NCAM in 80% of patients. There was no statistically significant relationship between tumor grade or invasiveness and immunohistochemical expression of these markers. Regarding gene expression, 50% of cases expressed CDH1, none expressed SNAI2, and 53.3% expressed NCAM1. There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1, SNAI2, and NCAM1. The absence of Slug overexpression and of E-cadherin and NCAM suppression suggests that expression of these markers is not associated with tumor invasiveness in GH-secreting pituitary adenomas.