DOI: 10.5061/dryad.7pvmcvdq9
title: Data from: Protection of pepper plants from drought by microbacterium sp. 3J1 by modulation of the plant's glutamine and α-ketoglutarate content: a comparative metabolomics approach

description: Desiccation-tolerant plants are able to survive for extended periods of
 time in the absence of water. The molecular understanding of the
 mechanisms used by these plants to resist droughts can be of great value
 for improving drought tolerance in crops. This understanding is especially
 relevant in an environment that tends to increase the number and intensity
 of droughts. The combination of certain microorganisms with
 drought-sensitive plants can improve their tolerance to water scarcity.
 One of these bacteria is Microbacterium sp. 3J1, an actinobacteria able to
 protect pepper plants from drought. In this study, we supplemented
 drought-tolerant and drought-sensitive plant rhizospheres with
 Microbacterium sp. 3J1 and analyzed their proteomes under drought to
 investigate the plant-microbe interaction. We also compare this root
 proteome with the proteome found in desiccation-tolerant plants. In
 addition, we studied the proteome of Microbacterium sp. 3J1 subjected to
 drought to analyze its contribution to the plant-microbe interaction. We
 describe those mechanisms shared by desiccation-tolerant plants and
 sensitive plants protected by microorganisms focusing on protection
 against oxidative stress, and production of compatible solutes, plant
 hormones, and other more specific proteins.
description: Green Pepper <i>Capsicum annuum</i><i>
 </i>L. cv. Maor<i> </i>plants inoculated and
 non-inoculated with the soil bacteria <i>Microbacterium</i>
 sp. 3J1<b> </b>and then, they were subjected to a dehydration
 treatment to simulate drought conditions. In
 addition, <i>Microbacterium</i> sp. 3J1 cultures were grown in
 tryptic soy broth (TSB) supplemented with 5% or 50% (w/v) PEG 8000
 to simulate different drought conditions. Soluble
 proteins of both, plants (roots) and bacterial cultures were isolated
 and precipitated by adding 50% (v/v) ice cold trichloroacetic acid
 (TCA). Total soluble protein were separated by two-dimensional
 polyacrylamide gel electrophoresis (2D-PGAGE) and geles images were
 analyzed by PDQuest Basic Software (Bio-Rad, Hercules, CA, USA). Spots
 differentially expressed were selected by two different criteria:
 Qualitative spots (presence/absence), and quantitative spots
 (<i>p</i> = ≤ 0.05) with a fold difference of 2 compared to
 the control. The spots containing
 the proteins of interest were cut using the EXQuest Spot Cutter (Bio-Rad).
 Gel pieces containing the proteins of interest were digested with Trypsin
 Gold Mass Spectrometry Grade (Promega) and tryptic digests of
 each spot were subjected to Mass Spectometry for protein
 identification. The MS and tandem MS (MS/MS) spectra were obtained using
 an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker-Daltonics) and
 processed using ProteinScape v3.1.3 (Bruker-Daltonics). The peptide mass fingerprint and
 MS/MS search were performed on NCBI database for Viridiplantae and
 <i>Capsicum annuum </i>searching<i> </i>for plant
 proteins. Bacterial proteins of <i>Microbacterium sp. </i>3J1
 were searched in databases for<i> Microbacteriae, Micrococci
 </i>and <i>Actinobacteria.</i> SwissProt database was
 used for all organisms, using MASCOT 2.4.0 software integrated together
 with ProteinScape software (Bruker-Daltonics).