DOI: 10.5061/dryad.bk3j9kd93
title: Trade-offs in above and belowground biomass allocation influencing seedling growth in a tropical forest

description: 1. Plants allocate biomass to different organs in response to resource
 variation for maximizing performance, yet we lack a framework that
 adequately integrates plant responses to the simultaneous variation in
 above and belowground resources. Although traditionally, the optimal
 partition theory (OPT) has explained patterns of biomass allocation in
 response to a single limiting resource, it is well known that in natural
 communities multiple resources limit growth. We study trade-offs involved
 in plant biomass allocation patterns and their effects on plant growth
 under variable below and aboveground resources –light, soil N, and P– for
 seedling communities. 2. We collected information on leaf, stem, and root
 mass fractions for more than 1,900 seedlings of 97 species paired with
 growth data and local-scale variation in abiotic resources from a tropical
 forest in China. 3. We identified two trade-off axes that define the mass
 allocation strategies for seedlings – allocation to photosynthetic vs.
 non-photosynthetic tissues and allocation to roots over stems – that
 responded to the variation in soil P and N and light. Yet, the allocation
 patterns did not always follow predictions of OPT in which plants should
 allocate biomass to the organ that acquires the most limiting resource.
 Limited soil N resulted in high allocation to leaves at expense of
 non-photosynthetic tissues, while the opposite trend was found in response
 to limited soil P. Also, co-limitation in above and belowground resources
 (light and soil P) led to mass allocation to stems at expense of roots.
 Finally, we found that growth increased under high light availability and
 soil P for seedlings that either invested more in photosynthetic over
 non-photosynthetic tissues or/and that allocated mass to roots at expense
 of stem. 4. Synthesis: Biomass allocation patterns to above and
 belowground tissues are described by two independent trade-offs that allow
 plants to have divergent allocation strategies (e.g., high root allocation
 at expense of stem or high leaf allocation at expense of allocation to
 non-photosynthetic tissues) and enhance growth under variable resources.
 Identifying the trade-offs driving biomass allocation is important to
 disentangle plant responses to the simultaneous variation in resources in
 diverse forest communities.
description: For each seedling plot, we sampled 50 g of top-soil (0-10 cm) immediately after the trait data collection was finished. The soil was collected from each of the corners and at the center of each 1-m<sup>2</sup> plot, then, mixed-together in a single sample and processed in the lab. Soil samples were posteriorly air-dried and sieved (2 mm). Total nitrogen (g/kg) was determined by total combustion using an auto-analyzer (Dumas combustion method, Vario MAX CN, Germany) and total phosphorus (g/kg) by inductively coupled plasma atomic emission spectrometry (ICP-AES). All the soil analyses were conducted at the Biogeochemical Laboratory at Xishuangbanna Tropical Botanical Garden.  To assess light conditions in the understory, we took hemispherical photographs at the center of each seedling plot. The pictures were taken at 1 m above the ground using a Nikon FC-E8 lens and a Nikon Coolpix 4500 camera (Nikkor, Nikon, Japan). All photographs were taken before sunrise between March and April 2014 and posteriorly analyzed using Gap Light Analyzer software (Frazer, Canham, & Lertzman, 2000) (http://www.caryinstitute.org/science-program/our-scientists/dr-charles-d-canham/gap-light-analyzer-gla).
description: Metadata: canopy.openness: Canopy openness
 (%) TN: Total nitrogen (%) TP: total
 phosphorus