DOI: 10.5061/dryad.np5hqc03h
title: Toxicokinetics and toxicodynamics data for fluazinam in the springtail Folsomia candida

description: The rise in global temperatures and increasing severity of heat waves pose
 significant threats to soil organisms, disrupting ecological balances in
 soil communities. Additionally, the implications of environmental
 pollution are exacerbated in a warmer world, as changes in temperature
 affect the uptake, transformation and elimination of toxicants, thereby
 increasing the vulnerability of organisms. Nevertheless, our understanding
 of such processes remains largely unexplored. The present study examines
 the impact of high temperatures on the uptake and effects of the fungicide
 fluazinam on the springtail Folsomia candida; Collembola, Isotomidae.
 Conducted under non-optimum but realistic high temperatures, the
 experiments revealed that increased temperature hampered detoxification
 processes in F. candida, enhancing the toxic effects of fluazinam. High
 temperatures and the fungicide exerted synergistic interactions, reducing
 F. candida’s reproduction and increasing adult mortality beyond what would
 be predicted by simple addition of the heat and chemical effects. These
 findings highlight the need to reevaluate the current ecological risk
 assessment and the regulatory framework in response to climate changes.
 This research enhances our understanding of how global warming affects the
 toxicokinetics and toxicodynamics (TK-TD) of chemicals in terrestrial
 invertebrates. In conclusion, our results suggest that adjustments to
 regulatory threshold values are necessary to address the impact of a
 changing climate.
description: Springtails (<em>Folsomia candida</em>) were exposed
 to fluazinam mixed into a standard soil type. Exposure proceeded for 28
 days at different temperatures. One experiment studied body concentration
 of fluazinam and major metabolites (toxicokinetics). Measured with high
 pressure liquid chromatography and mass spectronomy. A second experiment
 studied the effects of fluazinam on life history traits of springtails
 (toxicodynamics). Statistical modelling evaluated the nature of
 interactions between effects of temperature and the pesticide.
 Important: The raw data in this dataset need to be decoded with
 the provided R-Script since the data is only modified with the Script. The
 models in the dataset can be used as they are since they are already are
 already decoded and modelled accordingly to the described methods in
 Wehrli et al. 2024
description: **Dataset for Wehrli et al. 2024**
 [https://doi.org/10.5061/dryad.np5hqc03h](https://doi.org/10.5061/dryad.np5hqc03h) **Description of the data and file structure** Springtails (*Folsomia candida*) were exposed to fluazinam mixed into a standard soil type. According to the OECD 232 guideline. Exposure proceeded for 28 days at different temperatures. One experiment studied body concentration of fluazinam and major metabolites (toxicokinetics). A second experiment studied the effects of fluazinam on life history traits of springtails (toxicodynamics). Statistical modeling evaluated the nature of interactions between the effects of temperature and the pesticide. This data set provides the raw data, such as the AbosluteNumbers, weight of all replicates, length of all adults and juveniles, and two sheets for the randomization applied during the experiments. This data set also provides model outputs for the dose-response modelling as well as the Stressor addition model (SAM), Independent action (EA) and Effectconcentrationx System stress (ECxSyS) modelling. An R file is provided that uses the raw data to create all model outputs and to model all graphs used in the paper. **Files and variables** For all sheets, Concentration is in mg fluazinam/kg dw. soil (Except if it is stated that it is a metabolite in the header of the column) and temperature describe the exposure temperature (°C) during the experiments. **AbsoluteNumbersOECD232** Describes the total number of adults and juveniles per tested replicate and treatment for each temperature. Raw data for Figure 3 and Figure 6a. Absolute numbers of "Adults" and "Juveniles in each treatment are described as Original Code. ID Randomisation and Randomized are to identify the experimental treatments with the randomized list used to randomize during the testing phase. **DRCMortality** Describes the modeled survival of adults in relation to the concentration of fluazinam. Lower and upper CI are shown. Curves are shown in Figure 3. The data is the modeled number of surviving adults at each concentration. **DRCReproduction** Describes the modeled number of juveniles in relation to the concentration of fluazinam.  Lower and upper CI are shown. Curves shown in Figure 6a. The data is the modeled number of offspring at each concentration. **Peak Area toxicodynamics** This dataset includes the peak areas of the LC-MS/MS measurements of fluazinam in test soil of the toxicodynamics experiment (shown in Table 1). Fluazinam 1 is the quantifier and Fluazinam 2 is the qualifier, equally for the metabolites. Date describes the date the measurement was done. Day was an identifier for which experiment purely for R. Sampletype describes if it was a soil, collembolan or Standart sample. Sample Name describes the name during the measurement.  The following tabs describe Fluazinam 1 is the quantifier and Fluazinam 2 is the qualifier, equally for the metabolites. They are peak areas of the measured peaks. **Weight SoilRepro** Describing the amount of soil in the toxicodynamics experiment and solvent before and after the ASE extraction used for calculation of the concentrations of the analytes Sample Name describes the name during the measurement. All weights are in grams (g). **Calculated Conc.Repro** Calculated concentrations in the soil of the toxicodynamics experiment of the analytes were based on the peak area with a linear semi-quantification. The columns describe concentration (mg fluazinam/ kg dw soil) and error rates. Here sample type describes if it is either a Standard Curve or Soil sample. The red columns are below below the limit of detection (