DOI: 10.22002/qm2e2-g1j77
title: Spiroplasma_09aug27a_grid2_006

description: filament segmented by Sarah
description: <strong>Tilt Series Date:</strong> 2009-08-27</p>
<strong>Data Taken By:</strong> Morgan Beeby</p>
<strong>Species / Specimen:</strong> Spiroplasma melliferum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: 12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> grown by Bonnie data collected by Morgan processed by Bonnie</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in modified spiroplasma media with penicillin </p>
<strong>Sample Preparation:</strong> 4-minute glow discharge on Harrick glow discharger, Quantifoil R2/2 grids, 10 nm gold fiducials, "MORGAN5" Vitrobot settings</p>
  mba2009-08-27-5_slicer5480.jpg: Stacked Membrane mba2009-08-27-5_slicer5482.jpg: Stacked membrane mba2009-08-27-5_slicer5483.jpg: Ring
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mba2009-08-27-5/3dimage_8798/</p>09aug27a_grid2_006.mrc.rec,  Reconstruction (Pixel Size 1.9 nm), 91.6 MB  
        
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        mb2009-08-27-5-label-Filament01.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament02.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament03.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament04.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament05.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament06.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament07.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament08.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament09.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament10.am,  filament segmented by Sarah, 1.4 MB  
        
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        mb2009-08-27-5-label-Filament11.am,  filament segmented by Sarah, 1.4 MB  
        
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