cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7378280
title: Rapid colour changes in <i>Euglena sanguinea</i> (Euglenophyceae) caused by internal lipid globule migration

description: The accumulation of red pigments, frequently carotenoids, under chronic stress is a response observed in diverse kinds of eukaryotic photoautotrophs. It is thought that red pigments protect the chlorophyll located underneath by a light-shielding mechanism. However, the synthesis or degradation of carotenoids is a slow process and this response is usually only observed when the stress is maintained over long periods of time. In contrast, rapid colour changes have been reported in the euglenophyte <i>Euglena sanguinea</i>. Here we study the ecophysiological process behind this phenomenon through chlorophyll fluorescence, and pigment, colour and ultrastructural analyses. Reddening in <i>E. sanguinea</i> was due to the presence of a large amount of free and esterified astaxanthin (representing 80% of the carotenoid pool). The process was highly dynamic, shifting from green to red in 8 min (and vice-versa in 20 min). This change was not due to <i>de novo</i> carotenogenesis, but to the relocation of cytoplasmic lipid globules where astaxanthin accumulates. Thus, red globules were observed to migrate from the centre of the cell to peripheral locations when exposed to high light. Globule migration seems to be so efficient that other classical photoprotective mechanisms are not operative in this species. Despite the presence and operation of the diadino-diatoxanthin cycle, non-photochemical quenching was almost undetectable. Since <i>E. sanguinea</i> forms extensive floating colonies, reddening can be observed at a much greater scale than at the cellular level and the mechanism described here is one of the fastest and most dramatic colour changes attributable to photosynthetic organisms at cell and landscape level. In conclusion <i>E. sanguinea</i> shows an extremely dynamic and efficient photoprotective mechanism, based more on organelle migration than on carotenoid biosynthesis, which prevents excess light absorption by chlorophylls reducing the need for other protective processes related to energy dissipation.
DOI: 10.7284/129512
title: Water Temperature data collected during research cruise OC1704A using a Sea-Bird SBE-48 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378301.v1
title: PrzepioraT_rawdata

description: PrzepioraTomasz_rawdata
DOI: 10.6084/m9.figshare.7378280.v1
title: Rapid colour changes in <i>Euglena sanguinea</i> (Euglenophyceae) caused by internal lipid globule migration

description: The accumulation of red pigments, frequently carotenoids, under chronic stress is a response observed in diverse kinds of eukaryotic photoautotrophs. It is thought that red pigments protect the chlorophyll located underneath by a light-shielding mechanism. However, the synthesis or degradation of carotenoids is a slow process and this response is usually only observed when the stress is maintained over long periods of time. In contrast, rapid colour changes have been reported in the euglenophyte <i>Euglena sanguinea</i>. Here we study the ecophysiological process behind this phenomenon through chlorophyll fluorescence, and pigment, colour and ultrastructural analyses. Reddening in <i>E. sanguinea</i> was due to the presence of a large amount of free and esterified astaxanthin (representing 80% of the carotenoid pool). The process was highly dynamic, shifting from green to red in 8 min (and vice-versa in 20 min). This change was not due to <i>de novo</i> carotenogenesis, but to the relocation of cytoplasmic lipid globules where astaxanthin accumulates. Thus, red globules were observed to migrate from the centre of the cell to peripheral locations when exposed to high light. Globule migration seems to be so efficient that other classical photoprotective mechanisms are not operative in this species. Despite the presence and operation of the diadino-diatoxanthin cycle, non-photochemical quenching was almost undetectable. Since <i>E. sanguinea</i> forms extensive floating colonies, reddening can be observed at a much greater scale than at the cellular level and the mechanism described here is one of the fastest and most dramatic colour changes attributable to photosynthetic organisms at cell and landscape level. In conclusion <i>E. sanguinea</i> shows an extremely dynamic and efficient photoprotective mechanism, based more on organelle migration than on carotenoid biosynthesis, which prevents excess light absorption by chlorophylls reducing the need for other protective processes related to energy dissipation.
DOI: 10.7284/129511
title: Timeserver data collected during research cruise OC1704A using a Microsemi SyncServer S350 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378256
title: ESBAM 2012

description: Wellbeing and Health Survey of Older adults from Peru
DOI: 10.7284/129521
title: Anemometer data collected during research cruise OC1705A using a Gill WindObserver II instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/129517
title: Fluorometer data collected during research cruise OC1705A using a WET Labs WETStar instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378196
title: Supplementary tables A1–3. Two parallel magmatic belts with contrasting isotopic characteristics from southern Tibet to Myanmar: Zircon U–Pb and Hf isotopic constraints

description: Supplementary tables A1–3. Table A.1 Zircon U–Pb age data of analyzed samples from batholiths in Myanmar; Table A.2 Zircon Hf isotopic data of analyzed samples from batholiths in Myanmar; Table A.3 Whole rock Sr–Nd isotopic data of analyzed samples from batholiths in Myanmar.
DOI: 10.7284/129522
title: GNSS (Global Navigation Satellite System) data collected during research cruise OC1705A using a Furuno GP-90 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/129527
title: GNSS (Global Navigation Satellite System) data collected during research cruise OC1705A using a Ashtech ADU5 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378124
title: Regulation of Mcl-1 alternative splicing by hnRNP F, H1 and K in breast cancer cells

description: Myeloid cell leukemia-1 (Mcl −1) is one of the most frequently amplified genes in cancer, and its overexpression is associated with poor prognosis and drug resistance. As a member of the Bcl-2 family it is involved in the control of the mitochondrial (intrinsic) cell death pathway. Alternative splicing of the (<i>Mcl-1</i>) gene results in the expression of two functionally distinct proteins, the anti-apoptotic Mcl-1<sub>L</sub> (exon 2 included) and the pro-apoptotic Mcl-1<sub>S</sub> (exon 2 skipped). Our data shows that transfecting siRNAs that target hnRNP K and the hnRNP F/H family result in a switch in splicing towards the pro-apoptotic Mcl-1<sub>S</sub>. Specific binding sites for these and other Mcl-1 splicing factors were investigated and identified by RNA immunoprecipitation and through construction of a Mcl-1 minigene construct. Moreover, this study shows up to a 30 fold change in the levels of Mcl-1<sub>S</sub> can be achieved through double and triple knockdowns of the most significant RNA binding proteins involved in Mcl-1 splicing, as well as activation of the mitochondrial cell death pathway. Targeting the splicing process of Mcl-1 along with other apoptotic regulators provides an exciting new therapeutic target in cancer cells, and may provide a way to overcome therapy resistance.
DOI: 10.6084/m9.figshare.7378115
title: Evaluating model performance: towards a non-parametric variant of the Kling-Gupta efficiency

description: Goodness-of-fit measures are important for an objective evaluation of runoff model performance. The Kling-Gupta efficiency (<i>R</i><sub>KG</sub>), which has been introduced as an improvement of the widely used Nash-Sutcliffe efficiency, considers different types of model errors, namely the error in the mean, the variability, and the dynamics. The calculation of <i>R</i><sub>KG</sub> is implicitly based on the assumptions of data linearity, data normality, and the absence of outliers. In this study, we propose a modification of <i>R</i><sub>KG</sub> as an efficiency measure comprising non-parametric components, i.e. the Spearman rank correlation and the normalized flow–duration curve. The performances of model simulations for 100 catchments using the new measure were compared to those obtained using <i>R</i><sub>KG</sub> based on a number of statistical metrics and hydrological signatures. The new measure resulted overall in better or comparable model performances, and thus it was concluded that efficiency measures with non-parametric components provide a suitable alternative to commonly used measures.
DOI: 10.7284/129515
title: Gyrocompass data collected during research cruise OC1705A using a Sperry MK-37 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378115.v1
title: Evaluating model performance: towards a non-parametric variant of the Kling-Gupta efficiency

description: Goodness-of-fit measures are important for an objective evaluation of runoff model performance. The Kling-Gupta efficiency (<i>R</i><sub>KG</sub>), which has been introduced as an improvement of the widely used Nash-Sutcliffe efficiency, considers different types of model errors, namely the error in the mean, the variability, and the dynamics. The calculation of <i>R</i><sub>KG</sub> is implicitly based on the assumptions of data linearity, data normality, and the absence of outliers. In this study, we propose a modification of <i>R</i><sub>KG</sub> as an efficiency measure comprising non-parametric components, i.e. the Spearman rank correlation and the normalized flow–duration curve. The performances of model simulations for 100 catchments using the new measure were compared to those obtained using <i>R</i><sub>KG</sub> based on a number of statistical metrics and hydrological signatures. The new measure resulted overall in better or comparable model performances, and thus it was concluded that efficiency measures with non-parametric components provide a suitable alternative to commonly used measures.
DOI: 10.6084/m9.figshare.7378124.v2
title: Regulation of Mcl-1 alternative splicing by hnRNP F, H1 and K in breast cancer cells

description: Myeloid cell leukemia-1 (Mcl −1) is one of the most frequently amplified genes in cancer, and its overexpression is associated with poor prognosis and drug resistance. As a member of the Bcl-2 family it is involved in the control of the mitochondrial (intrinsic) cell death pathway. Alternative splicing of the (<i>Mcl-1</i>) gene results in the expression of two functionally distinct proteins, the anti-apoptotic Mcl-1<sub>L</sub> (exon 2 included) and the pro-apoptotic Mcl-1<sub>S</sub> (exon 2 skipped). Our data shows that transfecting siRNAs that target hnRNP K and the hnRNP F/H family result in a switch in splicing towards the pro-apoptotic Mcl-1<sub>S</sub>. Specific binding sites for these and other Mcl-1 splicing factors were investigated and identified by RNA immunoprecipitation and through construction of a Mcl-1 minigene construct. Moreover, this study shows up to a 30 fold change in the levels of Mcl-1<sub>S</sub> can be achieved through double and triple knockdowns of the most significant RNA binding proteins involved in Mcl-1 splicing, as well as activation of the mitochondrial cell death pathway. Targeting the splicing process of Mcl-1 along with other apoptotic regulators provides an exciting new therapeutic target in cancer cells, and may provide a way to overcome therapy resistance.
DOI: 10.6084/m9.figshare.7378124.v1
title: Regulation of Mcl-1 alternative splicing by hnRNP F, H1 and K in breast cancer cells.

description: Myeloid cell leukemia-1 (Mcl -1) is one of the most frequently amplified genes in cancer, and its overexpression is associated with poor prognosis and drug resistance. As a member of the Bcl-2 family it is involved in the control of the mitochondrial (intrinsic) cell death pathway. Alternative splicing of the (<i>Mcl-1</i>) gene results in the expression of two functionally distinct proteins, the anti-apoptotic Mcl-1<sub>L</sub> (exon 2 included) and the pro-apoptotic Mcl-1<sub>S</sub> (exon 2 skipped). Our data shows that transfecting siRNAs that target hnRNP K and the hnRNP F/H family result in a switch in splicing towards the pro-apoptotic Mcl-1<sub>S</sub>. Specific binding sites for these and other Mcl-1 splicing factors were investigated and identified by RNA immunoprecipitation and through construction of a Mcl-1 minigene construct. Moreover, this study shows up to a 30 fold change in the levels of Mcl-1<sub>S</sub> can be achieved through double and triple knockdowns of the most significant RNA binding proteins involved in Mcl-1 splicing, as well as activation of the mitochondrial cell death pathway. Targeting the splicing process of Mcl-1 along with other apoptotic regulators provides an exciting new therapeutic target in cancer cells, and may provide a way to overcome therapy resistance.
DOI: 10.7284/129516
title: PTU (Pressure, Temperature, Humidity) data collected during research cruise OC1705A using a Vaisala PTU300 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/129519
title: Radiometer data collected during research cruise OC1705A using a RMR Co. PRP instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/129520
title: Water Temperature data collected during research cruise OC1705A using a Sea-Bird SBE-38 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378088
title: Supplementary Material for: Ascertainment of Dementia Cases in the Spanish European Prospective Investigation into Cancer and Nutrition-Murcia Cohort

description: <b><i>Background:</i></b> Cohort studies generally focus on a particular disease, although they offer the possibility of evaluating different outcomes with minimal additional investment. The objective of this study was to describe the methodology used to assess dementia in the European Prospective Investigation into Cancer and Nutrition (EPIC)-Murcia study. <b><i>Methods:</i></b> The EPIC-Murcia cohort consists of 8,515 healthy participants (68% women, aged 30–70 years), recruited between 1992 and 1996 and followed up for over 20 years. Incident cases were ascertained by a 2-step protocol: a record linkage with health databases to identify potential events and a review of medical records of potential cases to validate incident cases. <b><i>Results:</i></b> Overall, 1,202 potential cases were identified, and 275 dementia cases were validated. Medical reports were the source of information in 243 cases, with complete neurological information in 227, and a high degree of certainty of the diagnosis in 229 cases. P70 (dementia code) and/or antidementia drugs and/or ICD codes identified 259 cases (sensitivity: 94.2%, 95% CI 90.7–96.6; specificity: 98.1%, 95% CI 97.8–98.4). <b><i>Conclusion:</i></b> Ascertainment of incident dementia in the EPIC-Murcia cohort study was feasible using information from medical records. This systematic 2-step validation protocol is proposed as a feasible way to ascertain dementia in cohort studies originally designed for other endpoints.
DOI: 10.7284/129525
title: Water Temperature data collected during research cruise OC1705A using a Sea-Bird SBE-48 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/129524
title: Timeserver data collected during research cruise OC1705A using a Microsemi SyncServer S350 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133170
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise OC1705B using a Hawaii UHDAS instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7378064
title: Supplementary Material for: Lung Carcinoids: Long-Term Surgical Results and the Lack of Prognostic Value of Somatostatin Receptors and Other Novel Immunohistochemical Markers

description: <b><i>Background/Aims:</i></b> Lung carcinoids (LCs) are often diagnosed at an early stage and surgical intervention becomes the next phase of treatment. To date, there is lack of long-term follow-up data after surgery and prognostication based on WHO classification criteria and evolving prognostic markers, particularly the expression of somatostatin receptors (SSR). <b><i>Methods:</i></b> We included 102 consecutive patients (72 women; age at baseline 51 ± 16 years [mean ± SD]) with LCs, who underwent thoracic surgery (<i>n</i> = 99) and/or laser treatment (<i>n</i> = 8). Hospital charts were reviewed for clinico-pathological parameters. Immunohistochemical (IHC) expression of SSR1–5 and other novel markers were studied with regard to their prognostic value. <b><i>Results:</i></b> Five- and 10-year overall survival (OS) was 96 and 83% respectively; relative survival (RS) was 101 and 93% respectively; and event-free survival (EFS) was 80 and 67% respectively. Independent prognostic factors for OS, RS and/or EFS were age at diagnosis, histopathological type and the presence of ipsilateral mediastinal subcarinal lymph node metastases. Macro-radicality of resective surgery and its extent were associated with increased OS and EFS. The IHC expression of SSR1–5 and other novel markers was not associated with OS or EFS. <b><i>Conclusion:</i></b> The long-term outcome of surgically treated patients with LCs is favourable. Age, histopathological type and ipsilateral mediastinal subcarinal lymph node status at baseline were independent prognostic factors for survival and disease recurrence or progression. The extent of surgery and operative macro-radicality also had an impact on prognosis. None of the IHC markers tested appeared to be associated with disease prognosis.
DOI: 10.7284/133179
title: Anemometer data collected during research cruise OC1705B using a Gill WindObserver II instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133173
title: Fluorometer data collected during research cruise OC1705B using a WET Labs WETStar instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133174
title: GNSS (Global Navigation Satellite System) data collected during research cruise OC1705B using a Furuno GP-90 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133178
title: GNSS (Global Navigation Satellite System) data collected during research cruise OC1705B using a Ashtech ADU5 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133169
title: Gyrocompass data collected during research cruise OC1705B using a Sperry MK-37 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7377989
title: Experiment 2 Survival.dat from Interactions between the sexual identity of the nervous system and the social environment mediate lifespan in <i>Drosophila melanogaster</i>

description: Sex differences in lifespan are ubiquitous, but the underlying causal factors remain poorly understood. Inter- and intrasexual social interactions are well known to influence lifespan in many taxa, but it has proved challenging to separate the role of sex-specific behaviours from wider physiological differences between the sexes. To address this problem, we genetically manipulated the sexual identity of the nervous system—and hence sexual behaviour—in <i>Drosophila melanogaster</i>, and measured lifespan under varying social conditions. Consistent with previous studies, masculinization of the nervous system in females induced male-specific courtship behaviour and aggression, while nervous system feminization in males induced male–male courtship and reduced aggression. Control females outlived males, but masculinized female groups displayed male-like lifespans and male-like costs of group living. By varying the mixture of control and masculinized females within social groups, we show that male-specific behaviours are costly to recipients, even when received from females. However, consistent with recent findings, our data suggest the courtship expression to be surprisingly low cost. Overall, our study indicates that nervous system-mediated expression of sex-specific behaviour <i>per se</i>—independent of wider physiological differences between the sexes, or the receipt of aggression or courtship—plays a limited role in mediating sex differences in lifespan.
DOI: 10.6084/m9.figshare.7377989.v1
title: Experiment 2 Survival.dat from Interactions between the sexual identity of the nervous system and the social environment mediate lifespan in <i>Drosophila melanogaster</i>

description: Sex differences in lifespan are ubiquitous, but the underlying causal factors remain poorly understood. Inter- and intrasexual social interactions are well known to influence lifespan in many taxa, but it has proved challenging to separate the role of sex-specific behaviours from wider physiological differences between the sexes. To address this problem, we genetically manipulated the sexual identity of the nervous system—and hence sexual behaviour—in <i>Drosophila melanogaster</i>, and measured lifespan under varying social conditions. Consistent with previous studies, masculinization of the nervous system in females induced male-specific courtship behaviour and aggression, while nervous system feminization in males induced male–male courtship and reduced aggression. Control females outlived males, but masculinized female groups displayed male-like lifespans and male-like costs of group living. By varying the mixture of control and masculinized females within social groups, we show that male-specific behaviours are costly to recipients, even when received from females. However, consistent with recent findings, our data suggest the courtship expression to be surprisingly low cost. Overall, our study indicates that nervous system-mediated expression of sex-specific behaviour <i>per se</i>—independent of wider physiological differences between the sexes, or the receipt of aggression or courtship—plays a limited role in mediating sex differences in lifespan.
DOI: 10.7284/133168
title: PTU (Pressure, Temperature, Humidity) data collected during research cruise OC1705B using a Vaisala PTU300 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133180
title: Radiometer data collected during research cruise OC1705B using a RMR Co. PRP instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/133175
title: Singlebeam Echosounder data collected during research cruise OC1705B using a Knudsen 3260 instrument system onboard the platform RV Oceanus

description: Original (unprocessed) data collected during a Field_expedition