DOI: 10.20348/storedb/1056/1094
title: Biomaterial from 6 animal experiments conducted at SUBI
title: Contract 75311

description: An overview is given on biomaterial from six selected animal experiments carried out at SUBI. These experiments were, ordered by SUBIs contract numbers:
- Contract 75311: Tritium oxide effect on DNA of animal tissues structure and metabolism - Biological material from 221 female Wistar rats;
- Contract 76323: Study of body regenerative process after chronic effect of external gamma-radiation and incorporated tritium - including 1224 male Wistar rats in 6 experimental groups;
- Contract 77329: Experimental therapy at Pu intake in animals and levels of its accumulation in humans  Biomaterial from 2025 Wistar rats; 
- Contract 86323: Experimental development of approaches to early detection of late radiation effect - Biomaterial from 1101 Wistar rats and 442 CBA mice. 
- Contract 89323: Search of criteria of elevated risk of development of radiation-induced tumors and ways of their modification  Biomaterial from 1058 male Wistar rats
- Contract 93302: Peculiarities of energy Pu biological effect depending on age   Biomaterial from 610 animals, paraffin-fixed (435) and in formalin (175);
Further details on the studies and on the biomaterial are given in the respective documents (which will be completed step by step, i.e. contract by contract).
The collection and collation of the information was done as part of Task 2.3 (Evaluation of the SUBI tissue archive and database as a potential part of the European archive) of the previous EURATOM-funded project STORE (Sustaining access to Tissues and data frOm Radiobiological Experiments; contract number 23228), which was coordinated by BfS, Germany (see http://cordis.europa.eu/project/rcn/89386_en.html) .
It has been shown in the STORE project, that the biological material from the experiments conducted by SUBI can still be used. The respective Standard Operating Procedures are available on the STORE website (http://www.storedb.org/store_v3/documents.jsp ).
description: CONTRACT 75311 (Internal SUBI number for reference purposes)
«Tritium oxide effect on DNA of animal tissues structure and metabolism» 
Performance period: 1975-1977 

Publications
Scientific research report (final). Principal investigator V.V. Kreslov, principal executive G.S. Mushkachyova, G.G. Rusinova, executive  V.S. Revina et al.  FIB-1,1977.  Inv. No. 1105.  68p., 15 tables, 20 fig., 26 references. Contract materials were sources for 9 papers (1 manuscript, 8 publications). 

Background
The information was gathered from 1224 male Wistar rats that were used in SUBIs experiment no. 75311. The collection and collation of the information was done as part of Task 2.3 (Evaluation of the SUBI tissue archive and database as a potential part of the European archive) of the previous EURATOM-funded project STORE (Sustaining access to Tissues and data frOm Radiobiological Experiments; contract number 23228), which was coordinated by BfS, Germany (see http://cordis.europa.eu/project/rcn/89386_en.html) .
The information given here is based upon SUBIs 42 months report.
It has been shown in the STORE project, that the biological material from the experiments conducted by SUBI can still be used. The respective Standard Operating Procedures are available on the STORE website (http://www.storedb.org/store_v3/documents.jsp ).

The Study
Study population:  276 female rats
Exposure: Single oral intake of tritium oxide, external gamma radiation, control
Outcome of interest: 
-	DNA function and structure during 37 days after single oral intake of tritium oxide
-	kinetics of tritium body clearance
-	average lifespan and late effects. 

Results
Changes in biosynthesis rate and content of nucleic acids, activity of DNA degradation ferments were noted. By DNA single-stranded break criteria relative efficiency of tritium oxide and γ-radiation was estimated. Tritium oxide administration (absorbed dose 11.4 Gy) resulted in reduction of average lifespan (419 days) compared to intact control (699 days) and increase of tumors mainly due to malignant neoplasms.

Biological material 
Biomaterial is available from 221 rats as 2854 paraffin-fixed samples. The preparation method is described in STOREDB File 10865, accessible through http://dx.doi.org/doi:10.20348/STOREDB/1041/1070 .
2805 samples from 181 rats are related to experiments on late effects of tritium oxide and external γ-radiation (see Table 1). 
49 samples from 40 rats are related to the experiment on dynamics of intestines cell elements damage in tritium tests (37 rats, 46 samples) and in control (3 rats, 3 samples). The samples contain material obtained after 3, 6, 12 and 24 hours (3НОН) and 6, 12 hours (control).

Contact information
If you want to use the material you should contact SUBI through inter_dep@subi.su. If you have any questions regarding this short summary report, please contact Dr. Bernd Grosche (bgrosche@t-online.de).