cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11870259.v1
title: Additional file 1 of Prospective isolation of chondroprogenitors from human iPSCs based on cell surface markers identified using a CRISPR-Cas9-generated reporter

description: Additional file 1: Figure S1. related to Figure 2 and 3. GO enrichment analysis of unsorted and sorted cells. (A) Top 10 GO terms (biological process) that were associated with each population in unsorted cells. (B) CD47 was highly expressed in SOX2/TTR+ cells. (C) Top 10 GO terms (biological process) that were associated with each population in sorted cells. Figure S2. related to Figure 2 and 3. Overall gene expression of sorted and unsorted chondroprogenitors. RT-qPCR reveals differences between sorted and unsorted chondroprogenitor cells in overall expression of (A) neurogenic, (B) mesenchymal, and (C) chondrogenic genes. Gene expression in reference to undifferentiated hiPSCs with housekeeping gene TBP. * p &lt; 0.05. *** p &lt; 0.001. Data represented as mean ± SEM. n = 4 samples/group. Figure S3 related to Figure 2 and 3. Expression profiles of pro-chondrogenic genes in sorted and unsorted chondroprogenitor cells. scRNA-seq reveals that sorted and unsorted cells had distinct gene expression patterns of several markers that were proposed to be pro-chondrogenic identified by previous studies. Figure S4 related to Fig. 5. Histology for matrix proteins. Safranin-O staining for sGAG showing pellets derived from sorted chondroprogenitor cells had more robust staining and homogenous cell morphology compared to pellets derived from unsorted cells in two individual experimental replicates. Scale bar = 200 μm. Inset scale bar = 400 μm. Figure S5 related to Figure 5. IHC labeling for COL6A1. There was more distributed labeling for COL6A1 in unsorted chondroprogenitor pellets compared to the localization around cells in sorted chondroprogenitor pellets. Scale bar = 200 μm. Inset scale bar = 400 μm. Figure S6 related to Figure 6. Alternative analysis of gene expression. Expression of chondrogenic genes ACAN, SOX9, and COL2A1, fibrocartilage and bone matrix marker COL1A1, and hypertrophic cartilage marker COL10A1 was significantly increased with sorting. CT value of gene of interest was normalized to CT value of housekeeping gene TBP for each sample. ** p &lt; 0.01. *** p &lt; 0.001. **** p &lt; 0.0001. Data represented as mean ± SEM. n = 6-7 per group: 2 experimental replicates, 3-4 technical replicates (pellets). Figure S7. Histology of pellets derived from in vitro expanded unsorted and sorted chondroprogenitors. Chondrogenic capacity was maintained after one passage of both unsorted and sorted chondroprogenitor cells as shown by staining for sGAG. There was more robust staining in pellets derived from sorted cells. Safranin-O staining for sGAG showed similar loss of chondrogenic capacity for both unsorted and sorted chondroprogenitor cells through four passages. Table S1. Antibodies used for flow cytometry and sorting. Table S2. Human primer sequences. Primers were used for RT-qPCR and are listed as 5’ to 3’.
DOI: 10.6084/m9.figshare.11870253.v1
title: Additional file 3 of Unveiling synapse pathology in spinal bulbar muscular atrophy by genome-wide transcriptome analysis of purified motor neurons derived from disease specific iPSCs

description: Additional file 3: Table S1. DEGs in heatmap (NT). Related to Fig. 5C. Table S2. DEGs in heatmap (HPF). Related to Fig. 5D. Table S3. Profiles of GSEA. Table S4. Gene ontology terms (down in NT). List of GO terms negatively enriched in SBMA-MNs of NT (FDR 2-fold) expression levels in SBMA-MNs than in control-MNs. Table S9. Primer sequences and cycling conditions for quantitative RT-PCR. Table S10. Antibodies for immunocytochemistry.
DOI: 10.6084/m9.figshare.11870217
title: Additional file 5 of TBCRC 002: a phase II, randomized, open-label trial of preoperative letrozole with or without bevacizumab in postmenopausal women with newly diagnosed stage 2/3 hormone receptor-positive and HER2-negative breast cancer

description: Additional file 5. Appendix 2. Summary statistics for all tested small RNA features in sequencing data (sorted by letozole/bevacizumab responder P).
DOI: 10.6084/m9.figshare.11870211
title: Additional file 4 of TBCRC 002: a phase II, randomized, open-label trial of preoperative letrozole with or without bevacizumab in postmenopausal women with newly diagnosed stage 2/3 hormone receptor-positive and HER2-negative breast cancer

description: Additional file 4. Appendix 1. LASSO selected classifiers and qPCR assay information.
DOI: 10.6084/m9.figshare.11870217.v1
title: Additional file 5 of TBCRC 002: a phase II, randomized, open-label trial of preoperative letrozole with or without bevacizumab in postmenopausal women with newly diagnosed stage 2/3 hormone receptor-positive and HER2-negative breast cancer

description: Additional file 5. Appendix 2. Summary statistics for all tested small RNA features in sequencing data (sorted by letozole/bevacizumab responder P).
DOI: 10.6084/m9.figshare.11870211.v1
title: Additional file 4 of TBCRC 002: a phase II, randomized, open-label trial of preoperative letrozole with or without bevacizumab in postmenopausal women with newly diagnosed stage 2/3 hormone receptor-positive and HER2-negative breast cancer

description: Additional file 4. Appendix 1. LASSO selected classifiers and qPCR assay information.
DOI: 10.6084/m9.figshare.11870157
title: Additional file 9 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S5. Differentially expressed genes (DEGs) identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells treated with Activin A or SB431542. (log2[fold-change ≥1]; adjusted p-value ≤0.05).
DOI: 10.6084/m9.figshare.11870157.v1
title: Additional file 9 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S5. Differentially expressed genes (DEGs) identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells treated with Activin A or SB431542. (log2[fold-change ≥1]; adjusted p-value ≤0.05).
DOI: 10.6084/m9.figshare.11870139
title: Additional file 6 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S3. Differentially expressed genes (DEGs) identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells vs. WT PC-iPS cells. (log2[fold-change ≥1]; adjusted p-value ≤0.05).
DOI: 10.6084/m9.figshare.11870145
title: Additional file 7 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S4. KEGG pathway enrichment analysis for differentially expressed genes identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells vs. WT PC-iPS cells.
DOI: 10.6084/m9.figshare.11870139.v1
title: Additional file 6 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S3. Differentially expressed genes (DEGs) identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells vs. WT PC-iPS cells. (log2[fold-change ≥1]; adjusted p-value ≤0.05).
DOI: 10.6084/m9.figshare.11870145.v1
title: Additional file 7 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S4. KEGG pathway enrichment analysis for differentially expressed genes identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells vs. WT PC-iPS cells.
DOI: 10.6084/m9.figshare.11870127
title: Additional file 4 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S2. Detail of genes expression of clustering Nanog, pEPS and embryos.
DOI: 10.6084/m9.figshare.11870127.v1
title: Additional file 4 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S2. Detail of genes expression of clustering Nanog, pEPS and embryos.
DOI: 10.6084/m9.figshare.11870085
title: Additional file 10 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S6. KEGG pathway enrichment analysis for differentially expressed genes (DEGs) identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells treated with Activin A or SB431542.
DOI: 10.6084/m9.figshare.11870085.v1
title: Additional file 10 of A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

description: : Table S6. KEGG pathway enrichment analysis for differentially expressed genes (DEGs) identified using RNA-Seq data obtained from NANOG tdTomato knock-in positive PC-iPS cells treated with Activin A or SB431542.
DOI: 10.6084/m9.figshare.11869545
title: Supplementary Materials

description: These are supplementary materials in my study.
DOI: 10.6084/m9.figshare.11869545.v1
title: Supplementary Materials

description: These are supplementary materials in my study.
DOI: 10.6084/m9.figshare.11869548
title: WN18

description: This WORDNET TENSOR DATA consists of a collection of triplets (synset, relation_type, triplet) extracted from WordNet 3.0 (http://wordnet.princeton.edu). This data set can be seen as a 3-mode tensor depicting ternary relationships between synsets.<br><br>The definitions file (wordnet-mlj12-definitions.txt) contains one synset per line with the following format: synset_id (a 8-digit unique identifier) intelligible name (word+POS_tag+sense_index), definition. The previous 3 pieces of information are separated by a tab ('\t').<br>All wordnet-mlj12-*.txt files contain one triplet per line, with 2 synset_ids and relation type identifier in a tab separated format. The first element is the synset_id of the left hand side of the relation triple, the third one is the synset_id of the right hand side and the second element is the name of the type of relations between them.<br>There are 40,943 synsets and 18 relation types among them. The training set contains 141,442 triplets, the validation set 5,000 and the test set 5,000.<br>All triplets are unique and we made sure that all synsets appearing in the validation or test sets were occurring in the training set.<br>The WN18.zip file contains the other files, with more compression than the default "download all".<br>
DOI: 10.6084/m9.figshare.11869503
title: Data for Grundy (2020) - JCCS

description: These are the data that were used for the following paper:<br>Grundy, J.G. The effects of bilingualism on executive functions: An updated quantitative analysis. <i>Journal of Cultural Cognitive Science</i>
DOI: 10.6084/m9.figshare.11869491
title: First report of colonization by Panstrongylus megistus (Burmeister, 1835) (Hemiptera, Reduviidae, Triatominae) in the Metropolitan Region of São Paulo, Brazil

description: Abstract We report the first known occurrence of Panstrongylus megistus (Burmeister, 1835) (Hemiptera, Reduviidae, Triatominae) in the Metropolitan Region of São Paulo, Brazil. In 2018, adult specimens were sent by residents to the competent authorities and, in the inspection of the property, a large focus associated with a marsupial nest was found. This finding reinforces the importance of the species in the state of São Paulo, serves as an alert for epidemiological surveillance and extends the species colonization area in the state of São Paulo.
DOI: 10.6084/m9.figshare.11869497
title: SORPTION ISOTHERMS AND ISOSTERIC HEAT OF PERICARP AND ENDOSPERM TISSUES OF ARABICA COFFEE FRUIT

description: ABSTRACT The aim of this study was to evaluate and model the hygroscopic equilibrium and isosteric heat curves of pericarp and endosperm tissues of arabica (Coffea arabica) coffee fruit, in different temperature and relative humidity conditions. Sorption isotherms were drawn under temperatures from 20°C to 70°C and relative humidity ranging between 10% and 90% until the product reached the equilibrium water content with the environment. The experiment was set up in a 4 × 4 factorial scheme (four relative humidity of the drying air and three tissues of the coffee cherry pericarp + endosperm [1: exocarp + part of the mesocarp, 2: mesocarp, 3: endocarp, 4: endosperm]), in a completely randomized design, with three repetitions. The results were examined by analysis of variance and regression using the STATISTICA 5.0 statistical software. Among the analyzed models, the ones that best fit the experimental data were modified GAB, for exocarp + part of mesocarp and mesocarp, modified Henderson, for endocarp, and Sabbab for endosperm. It was observed that, for all treatments, the lower water contents required a higher amount of energy to reach the equilibrium water content, and the integral isosteric heat decreased with the increasing equilibrium water content.
DOI: 10.6084/m9.figshare.11869491.v1
title: First report of colonization by Panstrongylus megistus (Burmeister, 1835) (Hemiptera, Reduviidae, Triatominae) in the Metropolitan Region of São Paulo, Brazil

description: Abstract We report the first known occurrence of Panstrongylus megistus (Burmeister, 1835) (Hemiptera, Reduviidae, Triatominae) in the Metropolitan Region of São Paulo, Brazil. In 2018, adult specimens were sent by residents to the competent authorities and, in the inspection of the property, a large focus associated with a marsupial nest was found. This finding reinforces the importance of the species in the state of São Paulo, serves as an alert for epidemiological surveillance and extends the species colonization area in the state of São Paulo.
DOI: 10.6084/m9.figshare.11869497.v1
title: SORPTION ISOTHERMS AND ISOSTERIC HEAT OF PERICARP AND ENDOSPERM TISSUES OF ARABICA COFFEE FRUIT

description: ABSTRACT The aim of this study was to evaluate and model the hygroscopic equilibrium and isosteric heat curves of pericarp and endosperm tissues of arabica (Coffea arabica) coffee fruit, in different temperature and relative humidity conditions. Sorption isotherms were drawn under temperatures from 20°C to 70°C and relative humidity ranging between 10% and 90% until the product reached the equilibrium water content with the environment. The experiment was set up in a 4 × 4 factorial scheme (four relative humidity of the drying air and three tissues of the coffee cherry pericarp + endosperm [1: exocarp + part of the mesocarp, 2: mesocarp, 3: endocarp, 4: endosperm]), in a completely randomized design, with three repetitions. The results were examined by analysis of variance and regression using the STATISTICA 5.0 statistical software. Among the analyzed models, the ones that best fit the experimental data were modified GAB, for exocarp + part of mesocarp and mesocarp, modified Henderson, for endocarp, and Sabbab for endosperm. It was observed that, for all treatments, the lower water contents required a higher amount of energy to reach the equilibrium water content, and the integral isosteric heat decreased with the increasing equilibrium water content.
DOI: 10.6084/m9.figshare.11869485
title: Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

description: Abstract Gastrointestinal nematodes are responsible for great economic losses in sheep raising, and their control has long been carried out almost exclusively by the administration of anthelmintics, which have led to serious resistance problems. In the search for alternative control measures, phytotherapic research is highlighted. The aim of this study was to evaluate the action of Anethum graveolens (dill) essential oil on different stages of Haemonchus contortus life cycle, as well its cytotoxicity MDBK (Madin-Darby bovine kidney) cells. H. contortus larvae and eggs were obtained from infected sheep feces, and essential oil extracted from plant seeds through the Clevenger apparatus. 9.4, 4.7, 2.35, 1.17. 0.58 and 0.29 mg/mL concentrations were evaluated. The Egg Hatch Inhibition (HI), Larval Development Inhibition (LDI) and Larval Migration Inhibition (LMI) techniques were used. Thybendazole 0.025 mg/mL in HI and Levamisole 0.02 mg/mL in the LDI and LMI tests were used as positive controls, while distilled water and a Tween 80 solution were used as positive negative controls. The inhibition results obtained for the highest oil concentration were: HI 100%, LDI 98.58% and LMI 63.7%, differing (�� &lt;0.05) from negative controls. Main A. graveolens oil components present in 95.93% of the total oil were Dihydrocarvone (39.1%), Carvone (22.24%), D-Limonene (16.84%), Apiol (10.49%) and Trans-dihydrocarvone (7.26%). Minimum A. graveolens essential oil concentrations required to inhibit 50% (IC50) of egg hatching, larval development and larval migration were 0.006 mg/mL, 2.536 mg/mL and 3.963 mg/mL, respectively. Cell viability in MDBK (Madin-Darby bovine kidney) cells, when incubated with A. graveolens essential oil, was 86% for the highest (9.4 mg/mL) and 99% for the lowest concentration (0.29 mg/mL). A. graveolens essential oil, according to the results obtained in this study, is a promising alternative in sheep gastrointestinal nematode control.
DOI: 10.6084/m9.figshare.11869488
title: ARTIFICIAL NEURAL NETWORKS FOR PREDICTION OF PHYSIOLOGICAL AND PRODUCTIVE VARIABLES OF BROILERS

description: ABSTRACT Due to a number of factors involving the thermal environment of a broiler cutting installation and its interaction with the physiological and productive responses of birds, artificial intelligence has been shown to be an interesting methodology to assist in the decision-making process. For this reason, the main aim of this work was to develop an artificial neural network (ANN) to predict feed conversion (FC), water consumption (Cwater), and cloacal temperature (tclo) of broilers submitted to different air dry-bulb temperatures (24, 27, 30, and 33°C) and durations (1, 2, 3, and 4 days) of thermal stress in the second week of the production cycle. Relative humidity and wind speed were fixed at 60% and 0.2 ms−1, respectively. The experimental data were used for the development of an ANN with supervised training using the Levenberg-Marquardt backpropagation algorithm. The ANN consisted of three input layers one hidden, and three output with sigmoidal tangent transfer functions with values between −1 and 1. The developed ANN has adequate predictive capacity, with coefficients of determination (R2) for tclo, FC, and Cwater of 0.79, 0.87, and 0.97, respectively. In this way, the proposed ANN can be used as a support for decision-making to trigger poultry heating systems for broiler breeding.
DOI: 10.6084/m9.figshare.11869485.v1
title: Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

description: Abstract Gastrointestinal nematodes are responsible for great economic losses in sheep raising, and their control has long been carried out almost exclusively by the administration of anthelmintics, which have led to serious resistance problems. In the search for alternative control measures, phytotherapic research is highlighted. The aim of this study was to evaluate the action of Anethum graveolens (dill) essential oil on different stages of Haemonchus contortus life cycle, as well its cytotoxicity MDBK (Madin-Darby bovine kidney) cells. H. contortus larvae and eggs were obtained from infected sheep feces, and essential oil extracted from plant seeds through the Clevenger apparatus. 9.4, 4.7, 2.35, 1.17. 0.58 and 0.29 mg/mL concentrations were evaluated. The Egg Hatch Inhibition (HI), Larval Development Inhibition (LDI) and Larval Migration Inhibition (LMI) techniques were used. Thybendazole 0.025 mg/mL in HI and Levamisole 0.02 mg/mL in the LDI and LMI tests were used as positive controls, while distilled water and a Tween 80 solution were used as positive negative controls. The inhibition results obtained for the highest oil concentration were: HI 100%, LDI 98.58% and LMI 63.7%, differing (�� &lt;0.05) from negative controls. Main A. graveolens oil components present in 95.93% of the total oil were Dihydrocarvone (39.1%), Carvone (22.24%), D-Limonene (16.84%), Apiol (10.49%) and Trans-dihydrocarvone (7.26%). Minimum A. graveolens essential oil concentrations required to inhibit 50% (IC50) of egg hatching, larval development and larval migration were 0.006 mg/mL, 2.536 mg/mL and 3.963 mg/mL, respectively. Cell viability in MDBK (Madin-Darby bovine kidney) cells, when incubated with A. graveolens essential oil, was 86% for the highest (9.4 mg/mL) and 99% for the lowest concentration (0.29 mg/mL). A. graveolens essential oil, according to the results obtained in this study, is a promising alternative in sheep gastrointestinal nematode control.
DOI: 10.6084/m9.figshare.11869479
title: Biological behavior of magnesium-substituted hydroxyapatite during bone repair

description: Abstract The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI - bone defect filled with HA microspheres; GII - bone defect filled with HA microspheres replaced with Mg; GIII - empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal.
DOI: 10.6084/m9.figshare.11869482
title: Resource use efficiency of cotton in improved vs conventional planting geometry with exogenous application of bio-stimulant and synthetic growth retardant

description: Abstract Plant growth regulators and improved planting density are the innovative techniques in the establishment of more productive cotton crop. A field study was planned to assess the role of growth regulators in the resource utilization efficiency of cotton cultivars under different row spacing at Agronomic Research Area, Bahauddin Zakariya University Multan and Usmania Agricultural Farm, Shujaabad during Kharif 2012. The study was comprised of cotton cultivars viz. CIM-573 and CIM-598, cultivated under conventional (75 cm), medium (50 cm) and improved ultra-narrow row spacing (25 cm) and foliar spray of growth regulators viz. moringa leaf extract (MLE) and mepiquat chloride (MC), either alone or in combination, distilled water as a control. The application of MLE alone and in combination (MLE + MC) showed the promoting effect on crop growth rate, net assimilation rate, leaf area index, leaf area duration, sympodial branches and number of bolls leading to higher seed cotton yield of both cotton cultivars grown under conventional row spacing. While application of MC averts the plant growth without considerably improving the productivity. MLE being rich source of growth promoting hormone and nutrients showed its potential to a far greater extent under conventional row spacing in efficient utilization of available resources compared to MC and distilled water.