cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8194901.v1
title: Effect of acetylsalicylic acid on total myenteric neurons in mice experimentally infected with Trypanosoma cruzi

description: Abstract: We investigated the effects of acetylsalicylic acid (ASA) on the total myenteric neuronal population in the descending colon in Trypanosoma cruzi-infected mice. Thirty-five male Swiss mice, 60 days old, were divided into a control group (C group), control group treated with ASA (CA group), infected group (I group), and infected group treated with ASA (IA group). A total of 1300 trypomastigotes of the Y strain of T. cruzi were intraperitoneally inoculated in the IA and I groups. The CA and IA groups were treated with ASA intraperitoneally. At 75 days post-infection (dpi), all of the animals were sacrificed. Neurons in the colon were stained with Giemsa, quantified, and measured. No difference in the course of infection was observed between the IA and I groups, reflected by the parasitemia curve. Acetylsalicylic acid treatment in the CA and IA groups did not alter the total number of myenteric neurons compared with the C and I groups. The CA and IA groups exhibited an increase in the nuclear area, cytoplasmic area, and neuronal body area compared with the C and I groups. Future studies should elucidate the mechanism of action of ASA against Chagas’ disease in the chronic phase.
DOI: 10.6084/m9.figshare.8194904
title: Undergraduate Medical Students’ Views on Team-Based Learning (TBL)

description: ABSTRACT Team-Based Learning (TBL) is an educational strategy based on active learning which has become an important alternative pedagogical proposal in the context of teaching methods used in medical education. The aim of this study was to evaluate students’ perceptions about the use of the TBL methodology in Medical School and to analyze the different aspects of the application of this teaching method. A specific questionnaire was applied to the students with 8 questions and dichotomized answers (Likert scale with 5 items). During the month of June 2017 second and fourth year undergraduate medical students underwent several sessions of teaching with the TBL method at the Municipal University of São Caetano do Sul (USCS) with focus on the following themes: sepsis; trauma; leukemia; colorectal cancer; and lung cancer. The sample was calculated on 193 participants considering a relative precision of 10% and a statistical significance of 5% for an estimate of 70% of positive responses (strongly agree and agree) to the statement “This TBL had a positive impact on my learning”. The analysis was performed with descriptive statistics and the relative and absolute frequencies of responses with a 95% confidence interval for each estimated point. The use of the method was perceived as positive in aspects of preference (regarding the traditional expository class), motivation, satisfaction and learning. TBL stood out as an excellent pedagogical method for learning among medical students. One of the advantages was the ability to develop skills in clinical reasoning and the possibility of building knowledge with its practical use. The active method was thus found to be an important methodological alternative when compared to the traditional method.
DOI: 10.6084/m9.figshare.8194904.v1
title: Undergraduate Medical Students’ Views on Team-Based Learning (TBL)

description: ABSTRACT Team-Based Learning (TBL) is an educational strategy based on active learning which has become an important alternative pedagogical proposal in the context of teaching methods used in medical education. The aim of this study was to evaluate students’ perceptions about the use of the TBL methodology in Medical School and to analyze the different aspects of the application of this teaching method. A specific questionnaire was applied to the students with 8 questions and dichotomized answers (Likert scale with 5 items). During the month of June 2017 second and fourth year undergraduate medical students underwent several sessions of teaching with the TBL method at the Municipal University of São Caetano do Sul (USCS) with focus on the following themes: sepsis; trauma; leukemia; colorectal cancer; and lung cancer. The sample was calculated on 193 participants considering a relative precision of 10% and a statistical significance of 5% for an estimate of 70% of positive responses (strongly agree and agree) to the statement “This TBL had a positive impact on my learning”. The analysis was performed with descriptive statistics and the relative and absolute frequencies of responses with a 95% confidence interval for each estimated point. The use of the method was perceived as positive in aspects of preference (regarding the traditional expository class), motivation, satisfaction and learning. TBL stood out as an excellent pedagogical method for learning among medical students. One of the advantages was the ability to develop skills in clinical reasoning and the possibility of building knowledge with its practical use. The active method was thus found to be an important methodological alternative when compared to the traditional method.
DOI: 10.6084/m9.figshare.8194907
title: REMOVAL OF BPA FROM LANDFILL LEACHATES USING FENTON-ADSORPTION PROCESS

description: The objective of this investigation was to identify and quantify the presence of Bisphenol-A (BPA) in raw and treated leachates with the Fenton-adsorption process. BPA is used as a monomer to produce epoxy resins and it is a compound classified as endocrine disruptor. For this reason, it is important to quantify its presence in effluents that will be disposed to the environment to avoid damage to human health and other organisms. The Fenton-adsorption process consisted in the implementation of an advanced oxidation using H2O2 and FeSO4 applied to the raw leachate and subsequently the application of the adsorption process through a column packed with activated carbon. The quantification of BPA was carried out by gas chromatography coupled to mass spectrometry, using liquid-liquid and liquid-solid extraction methods. A recovery percentage study was carried out to validate the extraction methods. Finally, it was found that the leached evaluated contained &gt;12 mg L-1 and at the end of the treatment its concentration was &lt;0.0174 mg L-1.
DOI: 10.6084/m9.figshare.8194907.v1
title: REMOVAL OF BPA FROM LANDFILL LEACHATES USING FENTON-ADSORPTION PROCESS

description: The objective of this investigation was to identify and quantify the presence of Bisphenol-A (BPA) in raw and treated leachates with the Fenton-adsorption process. BPA is used as a monomer to produce epoxy resins and it is a compound classified as endocrine disruptor. For this reason, it is important to quantify its presence in effluents that will be disposed to the environment to avoid damage to human health and other organisms. The Fenton-adsorption process consisted in the implementation of an advanced oxidation using H2O2 and FeSO4 applied to the raw leachate and subsequently the application of the adsorption process through a column packed with activated carbon. The quantification of BPA was carried out by gas chromatography coupled to mass spectrometry, using liquid-liquid and liquid-solid extraction methods. A recovery percentage study was carried out to validate the extraction methods. Finally, it was found that the leached evaluated contained &gt;12 mg L-1 and at the end of the treatment its concentration was &lt;0.0174 mg L-1.
DOI: 10.6084/m9.figshare.8194913
title: Assessment of a Fully Optimized DPX-Based Procedure for the Multiclass Determination of Pesticides in Drinking Water Using High-Performance Liquid Chromatography with Diode Array Detection

description: In this study, a disposable pipette extraction (DPX)-based procedure was fully optimized for the multiclass determination of 12 pesticides, with significant differences in their physicochemical properties, using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The variables optimized were type of extraction phase, sample pH, sample volume, number of extraction and desorption cycles, time of each cycle, type and volume of desorption solvent, and NaCl concentration of the samples. The analytical parameters of merit were determined under optimized conditions and satisfactory results were achieved, with limits of detection (LOQs) varying from 0.06 µg L-1 for carbofuran to 2.30 µg L-1 for carbendazim and correlation coefficients of 0.9923 to 0.9999. Relative recoveries ranged from 75 to 117%. Good results were achieved for intraday (n = 3) precision (1 to 20%) and interday (n = 9) precision (1 to 24%). The method was applied to the monitoring of 20 drinking water samples collected from water treatment plants.
DOI: 10.6084/m9.figshare.8194913.v1
title: Assessment of a Fully Optimized DPX-Based Procedure for the Multiclass Determination of Pesticides in Drinking Water Using High-Performance Liquid Chromatography with Diode Array Detection

description: In this study, a disposable pipette extraction (DPX)-based procedure was fully optimized for the multiclass determination of 12 pesticides, with significant differences in their physicochemical properties, using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The variables optimized were type of extraction phase, sample pH, sample volume, number of extraction and desorption cycles, time of each cycle, type and volume of desorption solvent, and NaCl concentration of the samples. The analytical parameters of merit were determined under optimized conditions and satisfactory results were achieved, with limits of detection (LOQs) varying from 0.06 µg L-1 for carbofuran to 2.30 µg L-1 for carbendazim and correlation coefficients of 0.9923 to 0.9999. Relative recoveries ranged from 75 to 117%. Good results were achieved for intraday (n = 3) precision (1 to 20%) and interday (n = 9) precision (1 to 24%). The method was applied to the monitoring of 20 drinking water samples collected from water treatment plants.
DOI: 10.6084/m9.figshare.8194916
title: Peripheral odontogenic fibroma in the mandibular gingiva: case report

description: ABSTRACT Peripheral odontogenic fibroma (POF) is a rare benign neoplasm of odontogenic mesenchymal origin that accounts for approximately 4.7% of all odontogenic tumors. This article reports the case of a 29-year-old woman who presented with a painless, reddish mass in the right vestibular mandibular gingiva between the second premolar and the first molar. Radiographic examination revealed small crestal bone loss in the region. Microscopic analysis revealed a tumor composed of cellular fibroblastic connective tissue intermingled with conspicuous islands and strands of apparently inactive odontogenic epithelium. The final diagnosis was POF. No clinical signs of recurrence were observed 22 months after surgical excision. POF may be clinically mistaken for other common gingival lesions, such as pyogenic granuloma, peripheral giant cell lesion, and peripheral ossifying fibroma. Conservative local excision has been suggested as the treatment of choice for POF. However, in view of the paucity of information on the biological behavior and recurrence rate of this tumor, long-term follow-up of patients is mandatory.
DOI: 10.6084/m9.figshare.8194916.v1
title: Peripheral odontogenic fibroma in the mandibular gingiva: case report

description: ABSTRACT Peripheral odontogenic fibroma (POF) is a rare benign neoplasm of odontogenic mesenchymal origin that accounts for approximately 4.7% of all odontogenic tumors. This article reports the case of a 29-year-old woman who presented with a painless, reddish mass in the right vestibular mandibular gingiva between the second premolar and the first molar. Radiographic examination revealed small crestal bone loss in the region. Microscopic analysis revealed a tumor composed of cellular fibroblastic connective tissue intermingled with conspicuous islands and strands of apparently inactive odontogenic epithelium. The final diagnosis was POF. No clinical signs of recurrence were observed 22 months after surgical excision. POF may be clinically mistaken for other common gingival lesions, such as pyogenic granuloma, peripheral giant cell lesion, and peripheral ossifying fibroma. Conservative local excision has been suggested as the treatment of choice for POF. However, in view of the paucity of information on the biological behavior and recurrence rate of this tumor, long-term follow-up of patients is mandatory.
DOI: 10.6084/m9.figshare.8194922
title: Brain Death: Knowledge and Opinion of Physicians from an Intensive Care Unit

description: ABSTRACT  Objectives  Verify the knowledge of ICU physicians about the diagnosis of brain death and the opinions of ICU physicians about organ donation.  Methods  Quantitative, exploratory, descriptive and cross-sectional study.  Methods  We interviewed 38 physicians at an adult ICU of a State hospital in the city of Rio de Janeiro. The data collection instrument was split into three parts: the first to gather professional information; the second was composed of nine closed-ended, multiple-choice questions, which addressed technical criteria for the diagnosis of brain death; a further seven questions asked for the interviewee’s opinion on brain death and organ donation.  Results  The study population had a mean age of 38.45 years (DP = 10.58). In relation to training time, the average was 11.87 years (DP = 8.94). The overall quantity of correct answers to the conceptual questions was 8.07 (DP = 0.78). Of the 38 participants, only 12 (31.57%) correctly answered all the questions. Two interviewees (5.2%) did not feel confident about conducting the clinical examination. No significant differences were found between the number of correct anwers and the age and/or gender of the interviewee.  Conclusion  Only the professional group of intensivists had participants who answered all the technical questions correctly. However, some basic issues require deeper discussion. It is important to incorporate disciplines that approach this subject in undergraduate courses in the health area, which may help diffuse educational attitudes. Such themes should also be extensively addressed in specialization courses in intensive care in order to improve training about the whole context involving brain death and the donation-transplant process, since this process must be absolutely watertight at every step.
DOI: 10.6084/m9.figshare.8194922.v1
title: Brain Death: Knowledge and Opinion of Physicians from an Intensive Care Unit

description: ABSTRACT  Objectives  Verify the knowledge of ICU physicians about the diagnosis of brain death and the opinions of ICU physicians about organ donation.  Methods  Quantitative, exploratory, descriptive and cross-sectional study.  Methods  We interviewed 38 physicians at an adult ICU of a State hospital in the city of Rio de Janeiro. The data collection instrument was split into three parts: the first to gather professional information; the second was composed of nine closed-ended, multiple-choice questions, which addressed technical criteria for the diagnosis of brain death; a further seven questions asked for the interviewee’s opinion on brain death and organ donation.  Results  The study population had a mean age of 38.45 years (DP = 10.58). In relation to training time, the average was 11.87 years (DP = 8.94). The overall quantity of correct answers to the conceptual questions was 8.07 (DP = 0.78). Of the 38 participants, only 12 (31.57%) correctly answered all the questions. Two interviewees (5.2%) did not feel confident about conducting the clinical examination. No significant differences were found between the number of correct anwers and the age and/or gender of the interviewee.  Conclusion  Only the professional group of intensivists had participants who answered all the technical questions correctly. However, some basic issues require deeper discussion. It is important to incorporate disciplines that approach this subject in undergraduate courses in the health area, which may help diffuse educational attitudes. Such themes should also be extensively addressed in specialization courses in intensive care in order to improve training about the whole context involving brain death and the donation-transplant process, since this process must be absolutely watertight at every step.
DOI: 10.6084/m9.figshare.8194928
title: Physico-chemical characterization and antioxidant capacity of the colombian berry (Vaccinium meridionale Swartz) with a high-polyphenol content: potential effects in people with metabolic syndrome

description: Abstract Colombian berry (agraz) has demonstrated a high antioxidant content in vitro. In the present study, it was elaborated and characterized an agraz nectar, designed a placebo with similar physicochemical and sensorial characteristics of the agraz nectar, but without polyphenols; and evaluated their antioxidant capacity both in vitro and in vivo in adults with cardiovascular risk factors. The polyphenolic concentration of the agraz nectar and its antioxidant capacity was superior than other reported for this fruit. A high content of total cyanidins was observed in the freeze-dried agraz, but not delphinin was detected by HPLC. For the intervention study, sixty-six subjects, with at least three cardiometabolic risk factors, consumed agraz nectar or placebo daily during 4 weeks each, separated by a 4-week washout period. Total phenol concentration and antioxidant capacity in the participants’ serum were not statistically different after consuming agraz nectar and placebo. However, women increased significantly their serum DPPH scavenging capacity after consuming agraz nectar, compared to placebo. This is one of the first studies showing data about the effects of this colombian berry on the antioxidant capacity in people with metabolic syndrome.
DOI: 10.6084/m9.figshare.8194928.v1
title: Physico-chemical characterization and antioxidant capacity of the colombian berry (Vaccinium meridionale Swartz) with a high-polyphenol content: potential effects in people with metabolic syndrome

description: Abstract Colombian berry (agraz) has demonstrated a high antioxidant content in vitro. In the present study, it was elaborated and characterized an agraz nectar, designed a placebo with similar physicochemical and sensorial characteristics of the agraz nectar, but without polyphenols; and evaluated their antioxidant capacity both in vitro and in vivo in adults with cardiovascular risk factors. The polyphenolic concentration of the agraz nectar and its antioxidant capacity was superior than other reported for this fruit. A high content of total cyanidins was observed in the freeze-dried agraz, but not delphinin was detected by HPLC. For the intervention study, sixty-six subjects, with at least three cardiometabolic risk factors, consumed agraz nectar or placebo daily during 4 weeks each, separated by a 4-week washout period. Total phenol concentration and antioxidant capacity in the participants’ serum were not statistically different after consuming agraz nectar and placebo. However, women increased significantly their serum DPPH scavenging capacity after consuming agraz nectar, compared to placebo. This is one of the first studies showing data about the effects of this colombian berry on the antioxidant capacity in people with metabolic syndrome.
DOI: 10.6084/m9.figshare.8194931
title: Anthocyanins from blackberry (Rubus fructicosus L.) impregnated in yam bean (Pachyrhizus erosus (L.) Urb.) by osmotic dehydration

description: Abstract Yam bean (Pachyrhizus erosus (L.) Urb.) tuber has a low carbohydrate content and contains several aminoacids, but contains few active compounds, such as vitamins and antioxidants. Anthocyanins are antioxidants found in Rubus fructicosus L. The osmotic drying is a technique that can be used to impregnate antioxidants in a matrix, so the objective of this study was to impregnate yam bean tuber slices with anthocyanin. The effect of temperature (40, 50 and 60oC), sucrose content (40, 50 and 60oBrix) and vacuum pulse (0, 300 and 600 mbar) on water loss (WL), solid gain (SG), color changes (ΔΕ) and anthocyanin concentration (ΔA) during osmotic drying of yam bean slices was determined. Polynomial equations were used to determine changes in WL, SG, ΔΕ and ΔA of yam bean slices after 6 h of osmotic drying. An osmotic solution at 60oC with a SC of 40oBrix, applying a vacuum pulse of 12 mbar maximized anthocyanin impregnation (6.67 mg anthocyanin per g initial dry matter) in tuber slices. The Page equation predicted optimum WL, SG and ΔA of yam bean tuber slices during osmotic drying. No relation was found between temperature, sucrose content and vacuum pulse with K and n parameters in the Page equation.
DOI: 10.6084/m9.figshare.8194931.v1
title: Anthocyanins from blackberry (Rubus fructicosus L.) impregnated in yam bean (Pachyrhizus erosus (L.) Urb.) by osmotic dehydration

description: Abstract Yam bean (Pachyrhizus erosus (L.) Urb.) tuber has a low carbohydrate content and contains several aminoacids, but contains few active compounds, such as vitamins and antioxidants. Anthocyanins are antioxidants found in Rubus fructicosus L. The osmotic drying is a technique that can be used to impregnate antioxidants in a matrix, so the objective of this study was to impregnate yam bean tuber slices with anthocyanin. The effect of temperature (40, 50 and 60oC), sucrose content (40, 50 and 60oBrix) and vacuum pulse (0, 300 and 600 mbar) on water loss (WL), solid gain (SG), color changes (ΔΕ) and anthocyanin concentration (ΔA) during osmotic drying of yam bean slices was determined. Polynomial equations were used to determine changes in WL, SG, ΔΕ and ΔA of yam bean slices after 6 h of osmotic drying. An osmotic solution at 60oC with a SC of 40oBrix, applying a vacuum pulse of 12 mbar maximized anthocyanin impregnation (6.67 mg anthocyanin per g initial dry matter) in tuber slices. The Page equation predicted optimum WL, SG and ΔA of yam bean tuber slices during osmotic drying. No relation was found between temperature, sucrose content and vacuum pulse with K and n parameters in the Page equation.
DOI: 10.6084/m9.figshare.8194934
title: Clinicopathological characteristics of surgically treated localized renal masses in patients previously exposed to chemotherapy

description: ABSTRACT  Purpose: To explore the potential association between renal mass characteristics and a history of chemotherapy.  Materials and methods: A retrospective review of records of patients surgically treated for a localized renal mass between 2000 and 2012 was undertaken following an institutional review board approval. Patients age and sex, renal mass clinical characteristics (radiological size and mode of presentation) and pathological characteristics (diagnosis, renal cell carcinoma subtype, Fuhrman grade and stage) were compared between patients with and without a history of chemotherapy, using Fisher's exact test, Student's t-test and Wilcoxon rank sum test. A multivariate logistic analysis was performed to evaluate the independent association of chemotherapy and tumor pathology.  Results: Of the 1,038 eligible patients, 33 (3%) had a history of chemotherapy. The distribution of clinical stage, renal mass diagnosis, renal cell carcinoma subtype, Fuhrman grade, pathological stage, sex and median age were similar between the general population and the chemotherapy group. However, the latter had a higher rate of incidental presentation (P = 0.003) and a significantly smaller median radiological tumor size (P = 0.01). In a subset analysis of T1a renal cell carcinoma, the chemotherapy group presented an increased rate of high Fuhrman grade (P = 0.03). On multivariate analysis adjusted for radiological tumor size, sex and age the chemotherapy cohort had a 3.92 higher odds for high Fuhrman grade.  Conclusion: Patients with a history of chemotherapy typically present with smaller renal masses that, if malignant, have higher odds of harboring a high Fuhrman grade and thus may not be suitable for active surveillance.