DOI: 10.5061/dryad.00v09
title: Data from: Gonadotropin-releasing hormone receptor (Gnrhr) gene knock out: normal growth and development of sensory, motor and spatial orientation behavior but altered metabolism in neonatal and prepubertal mice

description: Gonadotropin-releasing hormone (GnRH) is important in the control of
 reproduction, but its actions in non-reproductive processes are less well
 known. In this study we examined the effect of disrupting the GnRH
 receptor in mice to determine if growth, metabolism or behaviors that are
 not associated with reproduction were affected. To minimize the effects of
 other hormones such as FSH, LH and sex steroids, the neonatal-prepubertal
 period of 2 to 28 days of age was selected. The study shows that
 regardless of sex or phenotype in the Gnrhr gene knockout line, there was
 no significant difference in the daily development of motor control,
 sensory detection or spatial orientation among the wildtype, heterozygous
 or null mice. This included a series of behavioral tests for touch,
 vision, hearing, spatial orientation, locomotory behavior and muscle
 strength. Neither the daily body weight nor the final weight on day 28 of
 the kidney, liver and thymus relative to body weight varied significantly
 in any group. However by day 28, metabolic changes in the GnRH null
 females compared with wildtype females showed a significant reduction in
 inguinal fat pad weight normalized to body weight; this was accompanied by
 an increase in glucose compared with wildtype females shown by
 Student-Newman-Keuls Multiple Comparison test and Student's unpaired
 t tests. Our studies show that the GnRH-GnRHR system is not essential for
 growth or motor/sensory/orientation behavior during the first month of
 life prior to puberty onset. The lack of the GnRH-GnRHR axis, however, did
 affect females resulting in reduced subcutaneous inguinal fat pad weight
 and increased glucose with possible insulin resistance; the loss of the
 normal rise of estradiol at postnatal days 15–28 may account for the
 altered metabolism in the prepubertal female pups.
description: Pups.develop.behaviorDevelopmental behavior data for daily testing of mouse pups with GnRHR knock out. Tests for vision, touch, mobility, strength, and righting reflexes. Statistical assessment and figures generated using GraphPad Prism software. Used to generate Fig 2, 3, 4, & 5.Body wt.(tested vs untested mice)Developmental behavior data for daily testing of mouse pups with GnRHR knock out. Tests for body weight of animal tested daily vs animals not tested. Statistical assessment and figures generated using GraphPad Prism software. Used to generate Fig 6.Fat.kidney.liver.thymus % body weightDevelopmental behavior data for daily testing of mouse pups with GnRHR knock out. Tests for organ weight (% body weight) for inguinal, gonadal, scapular and retro-peritoneal fat pads, kidney, liver and thymus. Statistical assessment and figures generated using GraphPad Prism software. Used to generate Fig 7 & 9.Glucose.(tested vs untested mice)Developmental behavior data for daily testing of mouse pups with GnRHR knock out. Tests for blood glucose levels in tested and untested pups. Statistical assessment and figures generated using GraphPad Prism software. Used to generate Fig. 8.Body.wt.daily.2-28 - t-testsDevelopmental behavior data for daily testing of mouse pups with GnRHR knock out. Tests for daily body weights of experimental animals. Statistical assessment and figures generated using GraphPad Prism software. Used to generate Fig 1 T-test statistics.Body.wt.daily.2-28 - SNK testsDevelopmental behavior data for daily testing of mouse pups with GnRHR knock out. Tests for daily body weights of experimental animals. Statistical assessment and figures generated using GraphPad Prism software. Used to generate Fig 1 SNK statistics.