cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4264217.v1
title: Data from "The Emergent Worm: Dynamic Cellular Differentiation in the Caenorhabditis elegans embryogenesis"

description: Data from the manuscript "The Emergent Worm: Dynamic Cellular Differentiation in the Caenorhabditis elegans embryogenesis".<br>Contributors of primary data include Nikhil Bhatla (Worm Web.org), Dr. Stephen Larson (OpenWorm), and Mark Watts (OpenWorm)
DOI: 10.6084/m9.figshare.4264190
title: Learning from Data to Design Functional Materials without Inversion Symmetry (Datasets)

description: Datasets of n=1 Ruddlesden-Popper oxide chemistry with features<br>
DOI: 10.6084/m9.figshare.4264148
title: Graham_et_al_2016_Frontiers_2017_Env_Micro

description: <br>Raw (joined, paired-end Illumina MiSeq) 16S sequences for data in: Graham EB, Crump AR, Resch CT, Fansler S, Arntzen E, Kennedy DW, Fredrickson JK and Stegen JC (2016). Coupling spatiotemporal community assembly processes to changes in microbial metabolism. <i>Front. Microbiol.</i> <b>7</b>:1949. doi: 10.3389/fmicb.2016.01949<br>Graham, E. B., A. R. Crump, C. T. Resch, S. Fansler, E. Arntzen, D. W. Kennedy, J. K. Fredrickson, and J. C. Stegen. Deterministic influences exceed dispersal effects on hydrologically-connected microbiomes. <i>Environmental microbiology</i> (2017).<br><br>Unassembled reads are listed in un1 (forward) and un2 (reverse).<br>Contact: emily.graham@pnnl.gov<br>
DOI: 10.6084/m9.figshare.4264190.v1
title: Learning from Data to Design Functional Materials without Inversion Symmetry (Datasets)

description: Datasets of n=1 Ruddlesden-Popper oxide chemistry with features<br>
DOI: 10.6084/m9.figshare.4264148.v2
title: Graham_et_al_2016_Frontiers_2017_Env_Micro

description: <br>Raw (joined, paired-end Illumina MiSeq) 16S sequences for data in: Graham EB, Crump AR, Resch CT, Fansler S, Arntzen E, Kennedy DW, Fredrickson JK and Stegen JC (2016). Coupling spatiotemporal community assembly processes to changes in microbial metabolism. <i>Front. Microbiol.</i> <b>7</b>:1949. doi: 10.3389/fmicb.2016.01949<br>Graham, E. B., A. R. Crump, C. T. Resch, S. Fansler, E. Arntzen, D. W. Kennedy, J. K. Fredrickson, and J. C. Stegen. Deterministic influences exceed dispersal effects on hydrologically-connected microbiomes. <i>Environmental microbiology</i> (2017).<br><br>Unassembled reads are listed in un1 (forward) and un2 (reverse).<br>Contact: emily.graham@pnnl.gov<br>
DOI: 10.6084/m9.figshare.4264148.v1
title: Graham_et_al_2016_Frontiers

description: <br>Raw (joined, paired-end Illumina MiSeq) 16S sequences for data in: Graham EB, Crump AR, Resch CT, Fansler S, Arntzen E, Kennedy DW, Fredrickson JK and Stegen JC (2016). Coupling spatiotemporal community assembly processes to changes in microbial metabolism. <i>Front. Microbiol.</i> <b>7</b>:1949. doi: 10.3389/fmicb.2016.01949<br>Unassembled reads are listed in un1 (forward) and un2 (reverse).<br>Contact: emily.graham@pnnl.gov<br>
DOI: 10.6084/m9.figshare.4263920
title: Synthesis of some novel pyrazolo[1,5-<i>a</i>]quinazolines and their fused derivatives

description: New pyrazolo[1,5-<i>a</i>]quinazoline-3-carbonitriles <b>4a,b</b> were obtained via cyclocondensation of 5-amino-3-cyanomethyl-1<i>H</i>-pyrazole-4-carbonitrile (<b>1</b>) with enaminones of 1,3-cyclohexanedione derivatives <b>2a,b</b> in refluxing glacial acetic acid. Condensation of compounds <b>4a,b</b> with various aromatic aldehydes furnished the corresponding arylidene derivatives <b>6a–j</b>. On the other hand, condensation of <b>4a,b</b> with o-hydroxybenzaldehydes yielded the polyheterocyclic compounds <b>10a–h</b>. Coupling of compounds <b>4a,b</b> with aryldiazonium chlorides led to formation of 2-arylhydrazono derivatives <b>12a–h</b>. Also, reaction of compounds <b>4a,b</b> with phenyl isothiocyanate, followed by addition of ethyl chloroacetate and chloroacetonitrile, afforded the polyheterocyclic compounds based on pyrazolo[1,5-<i>a</i>]quinazoline core. The reaction of compounds <b>4a,b</b> with phenyl isothiocyanate and elemental sulfur gave the thiazole-2-thione derivatives <b>25a,b</b>. The reaction of enamines of compounds <b>4a,b</b> with each of hydrazine hydrate and guanidine hydrochloride afforded pyrazolo[4″,3″:5′,6′]pyrido[4′,3′:3,4]pyrazolo[1,5-<i>a</i>]quinazolin-8-ones <b>30a,b</b> and pyrimido[5″,4″:5′,6′]pyrido[4′,3′:3,4]pyrazolo[1,5-<i>a</i>]quinazolin-9(10H)-ones <b>33a,b</b>, respectively. The structures of all the newly synthesized compounds were elucidated by elemental analyses and spectral data. The plausible mechanisms have been postulated to account for their formation.
DOI: 10.6084/m9.figshare.4263917
title: Synthesis and antitumor activity evaluation of some novel pyrazolotriazine derivatives

description: 6-Aminopyrazolo[1,2-<i>a</i>][1,2,4]triazine-4,8-dione derivative <b>3</b> was obtained upon the reaction of the acid hydrazide derivative <b>2a</b> with ethyl cyanoacetate. The reactions of <b>3</b> with several electrophiles such as aldehydes, isatin, acetic anhydride, phenyl isocyanate, benzoyl isothiocyanate, and <i>p</i>-toluenesulfonyl chloride were studied. The structures of the newly synthesized compounds were established on the basis of IR, <sup>1</sup>H NMR, mass spectra, and elemental analyses. The antitumor activities of some selective compounds were examined against two cell lines as liver carcinoma cell line (HEPG-2) and human breast cancer cell line (MCF7).
DOI: 10.6084/m9.figshare.4263920.v2
title: Synthesis of some novel pyrazolo[1,5-<i>a</i>]quinazolines and their fused derivatives

description: New pyrazolo[1,5-<i>a</i>]quinazoline-3-carbonitriles <b>4a,b</b> were obtained via cyclocondensation of 5-amino-3-cyanomethyl-1<i>H</i>-pyrazole-4-carbonitrile (<b>1</b>) with enaminones of 1,3-cyclohexanedione derivatives <b>2a,b</b> in refluxing glacial acetic acid. Condensation of compounds <b>4a,b</b> with various aromatic aldehydes furnished the corresponding arylidene derivatives <b>6a–j</b>. On the other hand, condensation of <b>4a,b</b> with o-hydroxybenzaldehydes yielded the polyheterocyclic compounds <b>10a–h</b>. Coupling of compounds <b>4a,b</b> with aryldiazonium chlorides led to formation of 2-arylhydrazono derivatives <b>12a–h</b>. Also, reaction of compounds <b>4a,b</b> with phenyl isothiocyanate, followed by addition of ethyl chloroacetate and chloroacetonitrile, afforded the polyheterocyclic compounds based on pyrazolo[1,5-<i>a</i>]quinazoline core. The reaction of compounds <b>4a,b</b> with phenyl isothiocyanate and elemental sulfur gave the thiazole-2-thione derivatives <b>25a,b</b>. The reaction of enamines of compounds <b>4a,b</b> with each of hydrazine hydrate and guanidine hydrochloride afforded pyrazolo[4″,3″:5′,6′]pyrido[4′,3′:3,4]pyrazolo[1,5-<i>a</i>]quinazolin-8-ones <b>30a,b</b> and pyrimido[5″,4″:5′,6′]pyrido[4′,3′:3,4]pyrazolo[1,5-<i>a</i>]quinazolin-9(10H)-ones <b>33a,b</b>, respectively. The structures of all the newly synthesized compounds were elucidated by elemental analyses and spectral data. The plausible mechanisms have been postulated to account for their formation.
DOI: 10.6084/m9.figshare.4263920.v1
title: Synthesis of Some Novel Pyrazolo[1,5-<i>a</i>]Quinazolines and Their Fused Derivatives

description: New pyrazolo[1,5-a]quinazoline-3-carbonitriles <b>4a,b</b> were obtained via cyclocondensation of 5-amino-3-cyanomethyl-1H-pyrazole-4-carbonitrile (<b>1</b>) with enaminones of 1,3-cyclohexanedione derivatives <b>2a,b</b> in refluxing glacial acetic acid. The condensation of compounds <b>4a,b</b> with various aromatic aldehydes furnished the corresponding arylidene derivatives <b>6a-j</b>. On the other hand, condensation of <b>4a,b</b> with o-hydroxybenzaldehydes yielded the polyheterocyclic compounds <b>10a-h</b>. Coupling of compounds <b>4a,b</b> with aryldiazonium chlorides led to formation of 2-arylhydrazono derivatives <b>12a-h</b>. Also, reaction of compounds <b>4a,b</b> with phenyl isothiocyanate, followed by addition of ethyl chloroacetate and chloroacetonitrile afforded the polyheterocyclic compounds <b>based on pyrazolo[1,5-a]quinazoline core.</b> The reaction of compounds <b>4a,b</b> with phenyl isothiocyanate and elemental sulfur gave the thiazole-2-thione derivatives <b>25a,b</b>. The reaction of enamines of compounds <b>4a,b</b> with each of hydrazine hydrate and guanidine hydrochloride afforded pyrazolo[4″,3″:5′,6′]pyrido[4′,3′:3,4]pyrazolo[1,5-a]quinazolin-8-ones <b>30a,b</b> and pyrimido[5″,4″:5′,6′]pyrido[4′,3′:3,4]pyrazolo[1,5-a]quinazolin-9(10H)-ones <b>33a,b</b>, respectively. The structure of all the newly synthesized compounds was elucidated by elemental analyses and spectral data. The plausible mechanisms have been postulated to account for their formation.
DOI: 10.6084/m9.figshare.4263917.v1
title: Synthesis and Antitumor Activity Evaluation of Some Novel Pyrazolotriazine Derivatives

description: 6-Aminopyrazolo[1,2-a][1,2,4]triazine-4,8-dione derivative <b>3</b> was obtained upon the reaction of the acid hydrazide derivative <b>2a</b> with ethyl cyanoacetate. The reactions of <b>3</b> with several electrophiles such as aldehydes, isatin, acetic anhydride, phenyl isocyanate, benzoyl isothiocyanate and p-toluenesulphonyl chloride were studied. The structures of the newly synthesized compounds were established on the basis of IR, <sup>1</sup>H-NMR, mass spectra, and elemental analyses. The antitumor activities of some selective compounds were examined against two cell lines as liver carcinoma cell line (HEPG-2) and human breast cancer cell line (MCF7).
DOI: 10.6084/m9.figshare.4263905
title: Code and Data: Power laws and critical fragmentation in global forests

description: Source code for image processing and statistical analysis, and the patch size data files for the manuscript:<b><br></b><b>Power laws and critical fragmentation in global forests</b>. (2016) Saravia L.A. Doyle S.R. Bond-Lamberty B. bioRxiv 091751; doi: http://dx.doi.org/10.1101/091751<br>Zip Files:<br>Patch Sizes distribution by region, year and threshold from 2000 to 2015: these are binary files consisting on a vector of doubles, the R command used to read it is readBin(connection_file, "double", n = 10^8)<br><b>Africa_PatchSizes.zip</b><b>Northamerica_PatchSizes.zip</b><b>Southamerica_PatchSizes.zip</b><b>Eurasia_PatchSizes.zip</b><b>Oceania_PatchSizes.zip</b><b>Southasia_PatchSizes.zip</b><br><br><b>LargestPatchAnimations.zip</b> Animations of the two largest patches for all the regions defined in the paper. <br><b>PercolationAnimations.zip </b> Animation demonstrating percolation in a forest/non-forest model<br><b>Study_areas_definition.zip</b> These files needed to be unziped in a folder and the location added to the script data_input_script.m file<br>R markdown files:<br><br><b>Threshold_sensitivity.Rmd</b> # Fitting heavy tails to modis VCF, Smax & RSmax patch analysis. <br><b>Download_modis.Rmd</b> # Download modis files MOD44B version 051<b><br></b><b>Map_study_areas.Rmd</b> # Generate maps of study areas <b><br></b><b>Map_Max_patches.Rmd</b> # Generate GIF animations of maximum patch dynamics <br>R Files<b><br></b><b>map_fun.r </b># Map raster functions<b>power_fun.r </b># Function to fit continuos heavy tail distributions<br>MATLAB Files<br>Extract patch sizes from bin, and other analysis not used in the paper<br><b>data_input_script.m</b><b>GCF_spatial_analyses.m</b><b><br></b>PYTHON Files<br><strong>powlawfit.py :</strong> Call the Python powerlawpackage from command line <br><br><br> DATA Tables: csv and tab separated text files with model fits for the patch size distribution models.<br><strong>Fitted_Patch_models.csv</strong><strong><strong>Fitted_Patch_models.txt</strong></strong><strong><strong><br></strong></strong><strong><strong><br></strong></strong><br>
DOI: 10.6084/m9.figshare.4263896
title: NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection

description: Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of <i>Nek8</i> in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and <i>Nek8</i>-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, <i>Nek8</i>-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51.
DOI: 10.6084/m9.figshare.4263896.v1
title: NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection

description: Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of <i>Nek8</i> in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and <i>Nek8</i>-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, <i>Nek8</i>-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51.
DOI: 10.6084/m9.figshare.4263905.v1
title: Critical transitions and the fragmenting of global forests

description: Source code for image processing and statistical analysis, and the patch size data files for the manuscript:<b><br></b><b>Critical transitions and the fragmenting of global forests</b>. (2016) Saravia L.A. Doyle S.R. Bond-Lamberty B. bioRxiv 091751; doi: http://dx.doi.org/10.1101/091751<br>Zip Files:<br>Patch Sizes distribution by region and year from 2000 to 2014: these are binary files consisting on a vector of doubles, the R command used to read it is readBin(connection_file, "double", n = 10^6)<br><b>africa_PatchSizes.zip</b><b>northamerica_PatchSizes.zip</b><b>southamerica_PatchSizes.zip</b><b>eurasia_PatchSizes.zip</b><b>oceania_PatchSizes.zip</b><b>southasia_PatchSizes.zip</b><br><b><br></b><b>ShaliziCodePowerlaw.zip</b> Code from C. R. Shalizi to fit power exponential distribution.<br><br><b>LargestPatchAnimations.zip</b> Animations of the two largest patches for all the regions defined in the paper. <br><b>PercolationAnimations.zip </b> Animation demonstrating percolation in a forest/non-forest model<b><br></b>R markdown files: <br><b>Fit_heavy_tails.Rmd</b> # Fitting heavy tails to modis VCF<b>Download_modis.Rmd</b> # Download modis files MOD44B version 051<b>Map_study_areas.Rmd</b> # Generate maps of study areas <b>Map_Max_patches.Rmd</b> # Generate GIF animations of maximum patch dynamics <br>R Files<b><br></b><b>map_fun.r </b># Map raster functions<b>power_fun.r </b># Function to fit continuos heavy tail distributions<br>MATLAB Files<br>Extract patch sizes from bin, and other analysis not used in the paper<br><b>data_input_script.m</b><b>GCF_spatial_analyses.m</b><br> DATA Tables: csv and tab separated text files with model fits for the patch size distribution models.<br><strong>Fitted_Patch_models.csv</strong><strong><strong>Fitted_Patch_models.txt</strong></strong><strong><strong><br></strong></strong><strong><strong><br></strong></strong><br>
DOI: 10.6084/m9.figshare.4263905.v2
title: Code and Data: Critical transitions and the fragmenting of global forests

description: Source code for image processing and statistical analysis, and the patch size data files for the manuscript:<b><br></b><b>Critical transitions and the fragmenting of global forests</b>. (2016) Saravia L.A. Doyle S.R. Bond-Lamberty B. bioRxiv 091751; doi: http://dx.doi.org/10.1101/091751<br>Zip Files:<br>Patch Sizes distribution by region and year from 2000 to 2014: these are binary files consisting on a vector of doubles, the R command used to read it is readBin(connection_file, "double", n = 10^6)<br><b>africa_PatchSizes.zip</b><b>northamerica_PatchSizes.zip</b><b>southamerica_PatchSizes.zip</b><b>eurasia_PatchSizes.zip</b><b>oceania_PatchSizes.zip</b><b>southasia_PatchSizes.zip</b><br><b><br></b><b>ShaliziCodePowerlaw.zip</b> Code from C. R. Shalizi to fit power exponential distribution.<br><br><b>LargestPatchAnimations.zip</b> Animations of the two largest patches for all the regions defined in the paper. <br><b>PercolationAnimations.zip </b> Animation demonstrating percolation in a forest/non-forest model<b><br></b>R markdown files: <br><b>Fit_heavy_tails.Rmd</b> # Fitting heavy tails to modis VCF<b>Download_modis.Rmd</b> # Download modis files MOD44B version 051<b>Map_study_areas.Rmd</b> # Generate maps of study areas <b>Map_Max_patches.Rmd</b> # Generate GIF animations of maximum patch dynamics <br>R Files<b><br></b><b>map_fun.r </b># Map raster functions<b>power_fun.r </b># Function to fit continuos heavy tail distributions<br>MATLAB Files<br>Extract patch sizes from bin, and other analysis not used in the paper<br><b>data_input_script.m</b><b>GCF_spatial_analyses.m</b><br> DATA Tables: csv and tab separated text files with model fits for the patch size distribution models.<br><strong>Fitted_Patch_models.csv</strong><strong><strong>Fitted_Patch_models.txt</strong></strong><strong><strong><br></strong></strong><strong><strong><br></strong></strong><br>
DOI: 10.6084/m9.figshare.4263887
title: Real-world data on nivolumab treatment of non-small cell lung cancer

description: <b>Background:</b> Checkpoint inhibitors have proven effectiveness in clinical trials for non-small cell lung cancer (NSCLC) patients, but if this is congruent with routine patient care is discussed. We present real-world experience with the PD1-inhibitor nivolumab in NSCLC. <b>Patients and methods:</b> Patients with NSCLC were considered eligible for nivolumab treatment after one or more lines of chemotherapy, and when in reasonable performance status (PS) [Eastern Cooperative Oncology Group (ECOG) <b>Results:</b> Fifty-eight patients (30 females) commenced therapy in the period June–August 2015. Median age was 64.6 years (range 32.3–88.2). Twenty-four patients had squamous cell carcinoma and 32 adenocarcinoma, 38 had received two or more prior lines of therapy. Fourteen cases (24%) were in ECOG PS 2. After a medium observation time of 14.3 months, 13 (22%) are still in treatment. Median time to treatment failure (TTF) was 4.0 months, 34% were off treatment during the first two months. Median overall survival (OS) is 11.7 months. There was no difference in TTF or OS among patients with squamous versus non-squamous histology or between 1 versus &gt;1 prior line of therapy. Four patients (7%) were off treatment due to toxicity, none were grade 4 or 5. <b>Conclusion:</b> Nivolumab treatment outside clinical trials seems to perform as expected.
DOI: 10.6084/m9.figshare.4263905.v3
title: Code and Data: Critical transitions and the fragmenting of global forests

description: Source code for image processing and statistical analysis, and the patch size data files for the manuscript:<b><br></b><b>Critical transitions and the fragmenting of global forests</b>. (2016) Saravia L.A. Doyle S.R. Bond-Lamberty B. bioRxiv 091751; doi: http://dx.doi.org/10.1101/091751<br>Zip Files:<br>Patch Sizes distribution by region and year from 2000 to 2014: these are binary files consisting on a vector of doubles, the R command used to read it is readBin(connection_file, "double", n = 10^6)<br><b>africa_PatchSizes.zip</b><b>northamerica_PatchSizes.zip</b><b>southamerica_PatchSizes.zip</b><b>eurasia_PatchSizes.zip</b><b>oceania_PatchSizes.zip</b><b>southasia_PatchSizes.zip</b><br><b><br></b><b>ShaliziCodePowerlaw.zip</b> Code from C. R. Shalizi to fit power exponential distribution.<br><br><b>LargestPatchAnimations.zip</b> Animations of the two largest patches for all the regions defined in the paper. <br><b>PercolationAnimations.zip </b> Animation demonstrating percolation in a forest/non-forest model<b><br></b>R markdown files: <br><b>Fit_heavy_tails.Rmd</b> # Fitting heavy tails to modis VCF<b>Download_modis.Rmd</b> # Download modis files MOD44B version 051<b>Map_study_areas.Rmd</b> # Generate maps of study areas <b>Map_Max_patches.Rmd</b> # Generate GIF animations of maximum patch dynamics <br>R Files<b><br></b><b>map_fun.r </b># Map raster functions<b>power_fun.r </b># Function to fit continuos heavy tail distributions<br>MATLAB Files<br>Extract patch sizes from bin, and other analysis not used in the paper<br><b>data_input_script.m</b><b>GCF_spatial_analyses.m</b><br> DATA Tables: csv and tab separated text files with model fits for the patch size distribution models.<br><strong>Fitted_Patch_models.csv</strong><strong><strong>Fitted_Patch_models.txt</strong></strong><strong><strong><br></strong></strong><strong><strong><br></strong></strong><br>
DOI: 10.6084/m9.figshare.4263887.v2
title: Real-world data on nivolumab treatment of non-small cell lung cancer

description: <b>Background:</b> Checkpoint inhibitors have proven effectiveness in clinical trials for non-small cell lung cancer (NSCLC) patients, but if this is congruent with routine patient care is discussed. We present real-world experience with the PD1-inhibitor nivolumab in NSCLC. <b>Patients and methods:</b> Patients with NSCLC were considered eligible for nivolumab treatment after one or more lines of chemotherapy, and when in reasonable performance status (PS) [Eastern Cooperative Oncology Group (ECOG) <b>Results:</b> Fifty-eight patients (30 females) commenced therapy in the period June–August 2015. Median age was 64.6 years (range 32.3–88.2). Twenty-four patients had squamous cell carcinoma and 32 adenocarcinoma, 38 had received two or more prior lines of therapy. Fourteen cases (24%) were in ECOG PS 2. After a medium observation time of 14.3 months, 13 (22%) are still in treatment. Median time to treatment failure (TTF) was 4.0 months, 34% were off treatment during the first two months. Median overall survival (OS) is 11.7 months. There was no difference in TTF or OS among patients with squamous versus non-squamous histology or between 1 versus &gt;1 prior line of therapy. Four patients (7%) were off treatment due to toxicity, none were grade 4 or 5. <b>Conclusion:</b> Nivolumab treatment outside clinical trials seems to perform as expected.