DOI: 10.5061/dryad.zkh1893qx
title: One-component ionizable amphiphilic Janus dendrimers as a delivery platform for efficient mRNA vaccine development

description: There have been rapid advances in nucleoside-modified messenger RNA-based
 vaccines, particularly during the SARS-CoV2 pandemic, demonstrating a
 rapid and cost-effective approach for addressing infectious diseases. A
 major challenge remains in developing a delivery system that protects mRNA
 from degradation and facilitates its passage through tissue and cellular
 barriers. Current four-component lipid nanoparticles enable efficient
 endosomal escape and are a pivotal technology for the delivery of mRNA
 vaccines. However, challenges such as stability, availability, durability,
 and adverse effects persist. We have developed a self-assembling
 one-component ionizable amphiphilic Janus dendrimers (IAJD) delivery
 system with naturally occurring amino heads, capable of efficiently
 delivering mRNA to the spleen and lymph nodes.  This
 single-component system simplifies synthesis, reduces development
 complexity, and enable rapid global distribution of mRNA vaccines during
 pandemics. As a proof of concept, one-component IAJD97, formulated with
 mRNA encoding norovirus mRNA capsid protein, demonstrates the potential of
 IAJDs as efficient delivery platform for mRNA vaccines, advancing their
 effectiveness and expanding applications to improve public health
 outcomes.
description: # One-component ionizable amphiphilic Janus dendrimers as a delivery
 platform for efficient mRNA vaccine development Dataset DOI:
 [10.5061/dryad.zkh1893qx](https://doi.org/10.5061/dryad.zkh1893qx) ##
 Description of the data and file structure **Description of the data and
 file structure** The scope of this evaluation is to assess potential acute
 toxic effects associated with the experimental treatment under study. The
 samples evaluated include mouse heart, lung, kidney, liver, and spleen
 tissues fixed in 10% formalin. Samples were collected 24 hours post
 treatment with a single-component IAJD97 formulated with mRNA. There were
 five naive animals and six treated animals, comprising two dose groups (10
 µg and 30 µg mRNA per mouse). ### Files and variables #### File:
 Histology_score__table.csv **Description:** H&E staining of the main
 mouse organs was examined by a board-certified veterinary pathologist.
 Findings were reported as semi-quantitative pathology scores for each
 organ. ##### Variables * Description: * Naive 1: untreated mouse 1 * Naive
 2: untreated mouse 2 * Naive 3: untreated mouse 3 * Naive 4: untreated
 mouse 4 * Naive 5: untreated mouse 5 * 10 ug per mouse 9: mouse treated
 with 10 ug of mRNA * 10 ug per mouse 10: mouse treated with 10 ug of mRNA
 * 10 ug per mouse 11: mouse treated with 10 ug of mRNA * 30 ug per mouse
 6: mouse treated with 30 ug of mRNA * 30 ug per mouse 7: mouse treated
 with 30 ug of mRNA * 30 ug per mouse 8: mouse treated with 30 ug of mRNA
 #### File: Histology_score_table_legend.csv **Description:** Scoring
 system ##### Variables * 0 = unremarkable; 1 = minimal changes; 2 = mild
 changes; 3 = moderate changes; 4 = severe changes. ## Code/software n/a ##
 Access information Other publicly accessible locations of the data: * n/a
 Data was derived from the following sources: * n/a