cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21804498
title: ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369.sgd

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804498.v1
title: ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369.sgd

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804501
title: ExtFig1C-ExtFig3C_WB_Xrn1_R1_LG246.tiff

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804435.v1
title: ExtFig1B_WB_Tubulin_R1-R2-R3_LG386-390-397.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804501.v1
title: ExtFig1C-ExtFig3C_WB_Xrn1_R1_LG246.tiff

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804504
title: ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369_merge_annotated.tiff

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804504.v1
title: ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369_merge_annotated.tiff

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804507
title: ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369.tiff

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804507.v1
title: ExtFig1C-ExtFig3C_WB_Xrn1_R2_LG369.tiff

description: <em>Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock out/down of Xrn1. </em>
DOI: 10.6084/m9.figshare.21804426
title: ExtFig1B_WB_PA_R1-R2-R3_LG386-390-397.sgd

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804435
title: ExtFig1B_WB_Tubulin_R1-R2-R3_LG386-390-397.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804438
title: ExtFig1B_WB_Tubulin_R1-R2-R3_LG386-390-397.sgd

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804438.v1
title: ExtFig1B_WB_Tubulin_R1-R2-R3_LG386-390-397.sgd

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21800603
title: Fig6G_PCR_MDCK_R1_LG389.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21804426.v1
title: ExtFig1B_WB_PA_R1-R2-R3_LG386-390-397.sgd

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804429
title: ExtFig1B_WB_PA_R1-R2-R3_LG386-390-397.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804429.v1
title: ExtFig1B_WB_PA_R1-R2-R3_LG386-390-397.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804432
title: ExtFig1B_WB_PA_R1-R2-R3_LG386-390-397_merge_annotated.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804432.v1
title: ExtFig1B_WB_PA_R1-R2-R3_LG386-390-397_merge_annotated.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804441
title: ExtFig1B_WB_Tubulin_R1-R2-R3_LG386-390-397_merge_annotated.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21804441.v1
title: ExtFig1B_WB_Tubulin_R1-R2-R3_LG386-390-397_merge_annotated.tif

description: Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
DOI: 10.6084/m9.figshare.21800603.v1
title: Fig6G_PCR_MDCK_R1_LG389.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800606
title: Fig6G_PCR_MDCK_R1_LG389.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800606.v1
title: Fig6G_PCR_MDCK_R1_LG389.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800609
title: Fig6G_PCR_MDCK_R2_LG401_Fig6H_PCR_P3virus_R2-R3_LG394.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800612
title: Fig6G_PCR_MDCK_R2_LG401_Fig6H_PCR_P3virus_R2-R3_LG394_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800612.v1
title: Fig6G_PCR_MDCK_R2_LG401_Fig6H_PCR_P3virus_R2-R3_LG394_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800615
title: Fig6G_PCR_MDCK_R2_LG401_Fig6H_PCR_P3virus_R2-R3_LG394.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800615.v1
title: Fig6G_PCR_MDCK_R2_LG401_Fig6H_PCR_P3virus_R2-R3_LG394.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800618
title: Fig6G-6I_PCR_MDCK_A549_R3_LG403-404_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800618.v1
title: Fig6G-6I_PCR_MDCK_A549_R3_LG403-404_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800621
title: Fig6I_PCR_A549_R1_LG390_Fig6H_PCR_P3virus_R1_LG394.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800609.v1
title: Fig6G_PCR_MDCK_R2_LG401_Fig6H_PCR_P3virus_R2-R3_LG394.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800621.v1
title: Fig6I_PCR_A549_R1_LG390_Fig6H_PCR_P3virus_R1_LG394.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800627.v1
title: Fig6I_PCR_A549_R1_LG390_Fig6H_PCR_P3virus_R1_LG394.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800624
title: Fig6I_PCR_A549_R2_LG402.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800624.v1
title: Fig6I_PCR_A549_R2_LG402.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800627
title: Fig6I_PCR_A549_R1_LG390_Fig6H_PCR_P3virus_R1_LG394.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800630
title: Fig6I_PCR_A549_R2_LG402.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800630.v1
title: Fig6I_PCR_A549_R2_LG402.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800636
title: Fig6I_PCR_A549_R1_LG390_Fig6H_PCR_P3virus_R1_LG394_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800636.v1
title: Fig6I_PCR_A549_R1_LG390_Fig6H_PCR_P3virus_R1_LG394_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.