DOI: 10.5061/dryad.stqjq2cb0
title: Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails

description: Nutritional limitation is a common phenomenon in nature that leads to
 trade-offs among processes competing for limited resources. These
 trade-offs are mediated by changes in physiological traits such as growth
 factors and circulating lipids. However, studies addressing the
 sex-specific effect of nutritional deficiency on these physiological
 variables are limited in birds. We used dietary restriction to mimic the
 depletion of resources to various degrees and investigated sex-specific
 effects on circulating levels of insulin-like growth factor 1 (IGF-1) and
 triglycerides in Japanese quails (Coturnix japonica) subjected to ad
 libitum, 20%, 30% or 40% restriction of their daily requirement, for two
 weeks. We also explored the association of both physiological variables
 with body mass and egg production. While dietary restriction showed no
 effects on circulating IGF-1, this hormone exhibited a marked sexual
 difference, with females having 64.7% higher IGF-1 levels than males.
 Dietary restriction significantly reduced plasma triglyceride levels in
 both sexes. Females showed more than six-fold higher triglyceride levels
 than males. Triglyceride levels were positively associated with body mass
 in females while showed not association in males. Overall, our findings
 revealed sex-specific expression of physiological variables under dietary
 restriction conditions, which coincide with body size.
description: We used dietary restriction to mimic resource depletion and
 examined its effects on IGF-1 (ng/mL) and triglycerides (mmol/L) in
 Japanese quails. Quails were subjected to ad libitum feeding or 20%, 30%,
 and 40% restriction levels for 14 days. We colleced plasma samples and
 measured IGF-1 and triglycerides levels at the beginning of the experiment
 (day 0), on days 7 and on day 14 of the restriction period. We measured
 plasma IGF-1 levels using a competitive IGF-1 Enzyme-linked immunosorbent
 assay (ELISA) (https://doi.org/10.1007/s00265-020-2821-6). We mesured
 plasma triglyceride levels using a photometric method with a
 half-automatic analyser (Lab-Analyse, Orvostechnika Ltd., Budapest,
 Hungary).
description: # Data from: Sex-specific effects of dietary restriction on physiological
 variables in Japanese quails
 [https://doi.org/10.5061/dryad.stqjq2cb0](https://doi.org/10.5061/dryad.stqjq2cb0) The dataset includes plasma levels of insulin like growth factor 1 (IGF-1) and triglycerides from a dietary restriction experiment in Japanese quails. Quails aged nine weeks were subjected to either ad libitum feeding or 20%, 30%, and 40% restrictions for 14 days. Plasma samples were collected on day zero (initial), day seven (week 1), and day forteen (week 2) of the experiment period. Plasma levels of IGF-1 were measured using a competitive Enzyme-linked immunosorbent assay (ELISA), while triglyceride levels were determined using a photometric method.  ## Description of the data and file structure # 1. Title: Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails ## 2. Author Information ### A. Principal Investigator (Contact Information) ##### Name: Gebrehaweria K. Reda ``` Institution: University of Debrecen Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary Email: gebrek2000@gmail.com or gebrek2000@agr.unideb.hu ``` #### B. Co-investigator 1 ##### Name: Sawadi F. Ndunguru ``` Institution: University of Debrecen Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary : Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary ``` #### C. Co-investigator 2 ##### Name: Brigitta Csernus ``` Institution: University of Debrecen Address: Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary ``` #### **D. Co-investigator 3** ##### Name: James K. Lugata ``` Institution: University of Debrecen Address: Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary ``` ##### E. Co-investigator 4 ##### Name: Renáta Knop ``` Institution: University of Debrecen Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary ``` #### F. Co-investigator 5 ##### Name: Csaba Szabó ``` Institution: University of Debrecen Address: Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary ``` #### G. Supervisor ##### Name: Levente Czeglédi ``` Institution: University of Debrecen Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary Email: czegledi@agr.unideb.hu ``` #### H. Supervisor ##### Name:Ádám Z. Lendvai Institution: University of Debrecen ``` Address: Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary Email: azlendvai@gmail.com ``` #### 3. Data collected: 2021 **4. Geographic location of data collection**: Institute of Agricultural Research and Educational Farm of the University of Debrecen, Debrecen, Hungary. **5. Founding source**: National Research, Development and Innovation Office (OTKA K139021) ## ACCESS INFORMATION **Recommended citation for this dataset:** Reda et al. (2024), Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails. ## DATA & FILE OVERVIEW #### File List: ``` File 1 Name: Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails File 1 Description: The effect different dietary restriction levels on plasma levels of IGF-1 and triglycerides in adult Japanese quails (Coturnix japonica). ``` ## METHODOLOGICAL INFORMATION We used dietary restriction to mimic resource depletion and examined its effects on IGF-1 (ng/mL) and triglycerides (mmol/L) in Japanese quails. Quails were subjected to ad libitum feeding or 20%, 30%, and 40% restriction levels for 14 days. We colleced plasma samples and measured IGF-1 and triglycerides levels at the beginning of the experiment (day 0), on days 7 and on day 14 of the restriction period. We measured plasma IGF-1 levels using a competitive Enzyme-linked immunosorbent assay (ELISA) ([https://doi.org/10.1007/s00265-020-2821-6](https://doi.org/10.1007/s00265-020-2821-6). We mesured plasma triglyceride levels using a photometric method with a half-automatic analyser (Lab-Analyse, Orvostechnika Ltd., Budapest, Hungary). ## DATA-SPECIFIC INFORMATION 1\. Number of variables/columns: 10 2\. Number of rows: 193 3\. Variable List: ``` birdID sex block stagger session handlingtime treatment week igf1 trig ``` **4. Missing data codes:** na ##### 5. Abbreviations used: ``` birdID; bird identification number. sex; sex of birds block; experimental block stagger; numbers showing staggering batch session; sample collection session in one day sampling handlingtime; time from first aproach to the bird until final blood collection from that bird treatment; different dietary restriction levels. week; sampling and measurement time points igf1; circulating level of IGF-1 in ng/mL measured using ELISA trig; circulating level of triglycerides mmol/L, measured using photometric method ADL; ad libitum. DR20; 20% restricted. DR30; 30% restricted. DR40; 40% restricted. ``` ## Sharing/Access information This is a section for linking to other ways to access the data, and for linking to sources the data is derived from, if any. Links to other publicly accessible locations of the data: * This data was used for a manuscript in progress entitled "**Sex-specific effects of dietary restriction on physiological variables in Japanese quails**". The mansucript is accepted for publication in Ecology and evolution journal.  Data was derived from the following sources: Sex-specific effects of dietary restriction on physiological variables in Japanese quails ## Code/Software We used R v. 4.2.2 “Innocent and Trusting” software to statistically analyse and visualise our data. All the codes and the packeges used are described below.