DOI: 10.5061/dryad.h1m2442
title: Data from: Sequential horizontal gene transfers from different hosts in a widespread Eurasian parasitic plant, Cynomorium coccineum

description: Premise of the study: Parasites with large geographic ranges, and
 different hosts in parts of their range, might acquire
 horizontally-transferred genes (HGTs), which might sometimes leave a
 footprint of gradual host and range expansion. Cynomorium coccineum, the
 only member of the Saxifragales family Cynomoriaceae, is a root
 holoparasite that occurs in water-stressed habitats from western China to
 the Canary Islands. It parasitizes at least ten angiosperm families from
 different orders, some of them only in parts of its range. This parasite
 therefore offers an opportunity to trace HGTs as long as parasite/host
 pairs can be obtained and sequenced. Methods: By sequencing mitochondrial,
 plastid, and nuclear loci from parasite/host pairs from throughout the
 parasite’s range and with prior information from completely assembled
 mitochondrial and plastid genomes, we detected ten HGTs of five
 mitochondrial genes. Key Results: The ten HGTs appear to have occurred
 sequentially as C. coccineum expanded from East to West. Molecular-clock
 models yield Cynomorium stem ages between 66 and 156 Myr, with relaxed
 clocks converging on 66–67 Myr. Chinese Sapindales, probably Nitraria,
 were the first source of transferred genes, followed by Iranian and
 Mediterranean Caryophyllales. The most recently acquired gene appears to
 come from a Tamarix host in the Iberian Peninsula. Conclusion: Data on
 HGTs that have accumulated over the past 15 years, along with this
 discovery of multiple HGTs within a single widespread species, underline
 the need for more whole-genome data from parasite/host pairs to
 investigate if and how transferred copies coexist with, or replace, native
 functional genes. In compliance with data protection regulations, you may
 request that we remove your personal registration details at any time.
 (Use the following URL:
 https://www.editorialmanager.com/ajb/login.asp?a=r) Please contact the
 publication office if you have any questions.
description: Appendix_S2A_atp1_ed_sites_excl_359_1294The alignment underlying Fig. S2AAppendix_S2B_atp8_ed_sites_excl_57_506The alignment underlying Fig. S2BAppendix_S2C_ccmFn_ed_sites_excl_49_1935The alignment underlying Fig. S2CAppendix_S2D_cox2_exon_ed_sites_excl_51_773The alignment underlying Fig. S2DAppendix_S2E_cox2_intron_ed_sites_excl_47_1140The alignment underlying Fig. S2EAppendix_S2F_cox1_exon_ed_sites_excl_180_1307The alignment underlying Fig. S2FAppendix_S2G_cox1_intron_ed_sites_excl_65_1066The alignment underlying Fig. S2GAppendix_S2H_matR_255_2015The alignment underlying Fig. S2HAppendix_S2I_rps3_229_1173The alignment underlying Fig. S2IAppendix_S3A_nuclear_SMC2_gaps_excl_43_561The alignment underlying Fig. S3AAppendix_S3B_plastid_genes_94_1971The alignment underlying Fig. S3BAppendix_S3C_mito_genes_54_3568The alignment underlying Fig. S3CAppendix_S4A_matR_gaps_excl_13_1478.nexThe alignment underlying Fig. S4AAppendix_S4B_mito_genes_excl_matR_7_3499The alignment underlying Fig. S4BAppendix_S4C_nuclear_SMC2_7_1287The alignment underlying Fig. S4CAppendix_S4D_plastid_genes_11_5567The alignment underlying Fig. S4D