DOI: 10.5061/dryad.wpzgmsbzd
title: Data from: Genotype and social environment influence female-female interactions in a non-social insect

description: Factors shaping social interactions between females are understudied in
 non-social insects. We tested whether female-female investigation,
 aggression, and same-sex sexual behaviour (SSB) are influenced by
 genotypic and environmental factors in the Hawaiian field cricket,
 Teleogryllus oceanicus. Males ordinarily sing to attract females, but some
 are silenced by an X-linked mutation (flatwing) that protects males
 against an eavesdropping parasitoid fly and impacts female gene
 expression. To test how the rapid evolution of silence impacts female
 social behaviour, we matched or mismatched females by genotype. We
 observed them in paired trials exposed to male song or silence. The song
 treatment mimicked normal cricket populations versus those in which the
 flatwing has been fixed. Most female-female interactions involved social
 investigation characterised by un-escalated antennal contact. Aggression
 and SSB also occurred, but were less frequent. Female pairs interacted
 more in the presence of male song, and this plasticity was augmented when
 female genotypes matched. This implies that male song is a catalyst for
 female social interaction, and its evolved loss may reduce investment in
 female intrasexual interactions in a genotype-dependent manner. We
 advocate for wider investigation of the function and fitness consequences
 of female-female behaviour, a phenomenon likely to be neglected in many
 taxa.
description: # Genotype and social environment influence female-female interactions in
 a non-social insect Dataset DOI:
 [https://doi.org/10.5061/dryad.wpzgmsbzd](https://doi.org/10.5061/dryad.wpzgmsbzd) ## Description of the data and file structure ### Files and variables ### **File: Data\_occurrence\_frequency\_duration.csv** **Description:** Data from 10-minute behavioural trials involving female-female pairs of *Teleogyllus oceanicus* (conducted at 25 ± 2°C, under red light, in a 27 × 38 cm arena). The occurrence, frequency, and duration of antennal contact, biting, flight attempts, mounting, and reception of mounting were measured (per individual). ##### Variables * Dyad_ID: Unique ID for each dyad (individual pair), composed of 5 identifiers: Genotype Pair (A, B, or C), Presence of Song (Y or N), breeding line of one individual (1, 4, 5, 6, 8, or 9) breeding line of the other individual (1, 4, 5, 6, 8, or 9), replicate number (for that combination). * Individual_ID: unique ID for each experimental individual. The first digit represents the breeding line number (1, 4, 5, 6, 8, or 9). * Genotype: Nw (*normal-wing*) or Fw (*flat-wing*). * Trial_Mass(g): Mass (in grams) measured on the day of the trial. * Breeding_Line: Breeding line of each individual used in the trial. Lines 1, 5, and 8 are *normal-wing*, lines 4, 6, and 9 are *flat-wing* (information about line creation and maintenance in section [**2.1, Cricket origins and maintenance**)]. * Genotype_Pair: **A** - when both individuals are *normal-wing* (Nw), **B** - when one individual is *normal-wing* and the other is *flat-wing* (Fw), **C** - when both individuals are *flat-wing*. * Presence_of_Song: Presence of male *T. oceanicus* calling song, Y (Yes) or N (No). More information about the audio samples used is provided in section [**2.3 Experimental trials].** * Antennal_Contact_Occurrence: Y (Yes - antennal contact occurred at least once) or N (No - antennal contact did not occur). * Antennal_Contact_Frequency: Number of times an individual engaged in antennal contact.  * Antennal_Contact_Duration(s): Duration (in seconds) of each bout of antennal contact. * Biting_Occurrence: Y (Yes - biting occurred at least once) or N (No - biting did not occur). * Biting_Frequency: Number of times an individual bit.  * Biting_Duration(s): Duration (in seconds) of each biting bout. * Flight_Occurrence: Y (Yes -flight attempt occurred at least once) or N (No - flight attempt did not occur). * Flight_Frequency: Number of times an individual attempted a flight.  * Flight_Duration(s): Duration (in seconds) of each flight attempt. * Mounting_Occurrence: Y (Yes -mounting occurred at least once) or N (No - mounting did not occur). * Mounting Frequency: Number of times an individual mounted another.  * Mounting_Duration(s): Duration (in seconds) of each mount. * Reception_of_Mounting_Occurrence: Y (Yes - reception of mounting occurred at least once) or N (No - reception of mounting did not occur). * Reception_of_Mounting_Frequency: Number of times an individual received mounting from another.  * Reception_of_Mounting_Duration(s): Duration (in seconds) of each mounting reception. ### File: R\_code\_analyses Contains the R code for all analyses reported in the paper. ### File: R\_code\_figures Contains the R code used to generate all figures in the paper. #### File: Supplementary material (uploaded to Zenodo) Table S1 - Model expressions of all statistical analyses performed. Table S2 - Results of ICC analysis for 2 raters, n=20, R threshold of roughly 0.7. Table S3 - Results of iteratively run models (n = 1000) for all occurrence analyses. Table S4 - Results of iteratively run models (n = 1000) for antennal contact frequency. Table S5 - Results of iteratively run models (n = 1000) for antennal contact duration. Table S6 - Results of iteratively run models (n = 1000) for biting and flight attempt frequency analyses.