DOI: 10.5061/dryad.25b8f
title: Data from: Critical thermal limits affected differently by developmental and adult thermal fluctuations

description: Means and variances of the environmental thermal regime play an important
 role in determining the fitness of terrestrial ectotherms. Adaptive
 phenotypic responses induced by heterogeneous temperatures have been shown
 to be mediated by molecular pathways independent of the classic heat shock
 responses, however, an in-depth understanding of plasticity induced by
 fluctuating temperatures is still lacking. We investigated high and low
 temperature acclimation induced by fluctuating thermal regimes at two
 different mean temperatures, at two different amplitudes of fluctuation
 and across the developmental and adult life stages. For developmental
 acclimation, we found mildly detrimental effects of high amplitude
 fluctuations for critical thermal minima, while the critical thermal
 maxima showed a beneficial response to higher amplitude fluctuations. For
 adult acclimation involving shifts between fluctuating and constant
 regimes, cold tolerance was shown to be dictated by developmental
 temperature conditions irrespective of the adult treatments, while the
 acquired heat tolerance was readily lost when flies developed at
 fluctuating temperature were shifted to a constant regime as adults.
 Interestingly, we also found that effect of fluctuations at any life stage
 was gradually lost with prolonged adult maintenance suggesting a more
 prominent effect of fluctuations during developmental compared to adult
 acclimation in Drosophila melanogaster.
description: CTmax_2d_4_19,23CTmax_2d_4_19,23: 2day old flies developed at average temperatures 19°C and 23°C, with an amplitude of +/- 4°C, assayed for CTmaxCTmax_2d_8_19,23CTmax_2d_4_19,23: 2day old flies developed at average temperatures 19°C and 23°C, with an amplitude of +/- 4°C, assayed for CTmaxCTmin_2d_4_19,23CTmin_2d_4_19,23: 2day old flies developed at average temperatures 19°C and 23°C, with an amplitude of +/- 4°C, assayed for CTminCTmin_2d_8_19,23CTmin_2d_8_19,23: 2day old flies developed at average temperatures 19°C and 23°C, with an amplitude of +/- 8°C, assayed for CTminCTmax_5d_4_19,23CTmax_5d_4_19,23: 5day old flies developed at average temperatures 19°C and 23°C, with an amplitude of +/- 4°C and shifted as adults to opposite treatment regime, assayed for CTmaxCTmin_5d_4_19,23CTmin_5d_4_19,23: 5day old flies developed at average temperatures 19°C and 23°C, with an amplitude of +/- 4°C and shifted as adults to opposite treatment regime, assayed for CTminCTmax_5d_8_19CTmax_5d_8_19: 5day old flies developed at average temperature 19°C, with an amplitude of +/- 8°C and shifted as adults to opposite treatment regime, assayed for CTmaxCTmax_5d_8_23CTmax_5d_8_23: 5day old flies developed at average temperature 23°C, with an amplitude of +/- 8°C and shifted as adults to opposite treatment regime, assayed for CTmaxCTmin_5d_8_19CTmin_5d_8_19: 5day old flies developed at average temperature 19°C, with an amplitude of +/- 8°C and shifted as adults to opposite treatment regime, assayed for CTminCTmin_5d_8_23CTmin_5d_8_23: 5day old flies developed at average temperature 23°C, with an amplitude of +/- 8°C and shifted as adults to opposite treatment regime, assayed for CTminCTmax_9d_4_19CTmax_9d_4_19: 9day old flies developed at average temperature 19°C, with an amplitude of +/- 4°C and shifted as adults to opposite temperature regime, assayed for CTmaxCTmax_9d_4_23CTmax_9d_4_23: 9day old flies developed at average temperature 23°C, with an amplitude of +/- 4°C and shifted as adults to opposite temperature regime, assayed for CTmaxCTmin_9d_4_19CTmin_9d_4_19: 9day old flies developed at average temperature 19°C, with an amplitude of +/- 4°C and shifted as adults to opposite temperature regime, assayed for CTminCTmin_9d_4_23CTmin_9d_4_23: 9day old flies developed at average temperature 23°C, with an amplitude of +/- 4°C and shifted as adults to opposite temperature regime, assayed for CTminCTmax_9d_8_19CTmax_9d_8_19: 9day old flies developed at average temperature 19°C, with an amplitude of +/- 8°C and shifted as adults to opposite temperature regime, assayed for CTmaxCTmax_9d_8_23CTmax_9d_8_23: 9day old flies developed at average temperature 23°C, with an amplitude of +/- 8°C and shifted as adults to opposite temperature regime, assayed for CTmaxCTmin_9d_8_19CTmin_9d_8_19: 9day old flies developed at average temperature 19°C, with an amplitude of +/- 8°C and shifted as adults to opposite temperature regime, assayed for CTminCTmin_9d_8_23CTmin_9d_8_23: 9day old flies developed at average temperature 23°C, with an amplitude of +/- 8°C and shifted as adults to opposite temperature regime, assayed for CTmin