cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14287363.v1
title: Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology

description: Abstract Background: The production of antivenom from immunized animals is an established treatment for snakebites; however, antibody phage display technology may have the capacity to delivery results more quickly and with a better match to local need. Naja oxiana, the Iranian cobra, is a medically important species, responsible for a significant number of deaths annually. This study was designed as proof of principle to determine whether recombinant antibodies with the capacity to neutralize cobra venom could be isolated by phage display. Methods: Toxic fractions from cobra venom were prepared by chromatography and used as targets in phage display to isolate recombinant antibodies from a human scFv library. Candidate antibodies were expressed in E. coli HB2151 and purified by IMAC chromatography. The selected clones were analyzed in in vivo and in vitro experiments. Results: Venom toxicity was contained in two fractions. Around a hundred phage clones were isolated against each fraction, those showing the best promise were G12F3 and G1F4. While all chosen clones showed low but detectable neutralizing effect against Naja oxiana venom, clone G12F3 could inhibit PLA2 activity. Conclusion: Therefore, phage display is believed to have a good potential as an approach to the development of snake antivenom.
DOI: 10.22008/fk2/oiajvo/fvleqa
title: snow_specific_area.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.22008/fk2/oiajvo/okbihc
title: al.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287361.v1
title: Toxicological effects of bioactive peptide fractions obtained from Bothrops jararaca snake venom on the structure and function of mouse seminiferous epithelium

description: Abstract Background: Pathogenesis of Bothrops envenomations is complex and despite numerous studies on the effects of this snake venom on various biological systems, relatively little is known about such effects on the male reproductive system. In the present study, the toxicological outcomes of the low molecular weight fraction (LMWF) of B. jararaca snake venom - containing a range of bioactive peptides - were investigated on the dynamics and structure of the seminiferous epithelium and 15P-1 Sertoli cells viability. Methods: LMWF (5 µg/dose per testis) venom was administered in male Swiss mice by intratesticular (i.t.) injection. Seven days after this procedure, the testes were collected for morphological and morphometric evaluation, distribution of claudin-1 in the seminiferous epithelium by immunohistochemical analyses of testes, and the nitric oxide (NO) levels were evaluated in the total extract of the testis protein. In addition, the toxicological effects of LMWF and crude venom (CV) were analyzed on the 15P-1 Sertoli cell culture. Results: LMWF induced changes in the structure and function of the seminiferous epithelium without altering claudin-1 distribution. LMWF effects were characterized especially by lost cells in the adluminal compartment of epithelium (spermatocytes in pachytene, preleptotene spermatocytes, zygotene spermatocytes, and round spermatid) and different stages of the seminiferous epithelium cycle. LMWF also increased the NO levels in the total extract of the testis protein and was not cytotoxic in concentrations and time tested in the present study. However, CV showed cytotoxicity at 10 μg/mL from 6 to 48 h of treatment. Conclusions: The major finding of the present study was that the LMWF inhibited spermatozoa production; principally in the spermiogenesis stage without altering claudin-1 distribution in the basal compartment. Moreover, NO increased by LMWF induce open of complexes junctions and release the germ cells of the adluminal compartment to the seminiferous tubule.
DOI: 10.22008/fk2/oiajvo/38rnj4
title: albedo_bb_planar_sw.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287360.v1
title: Venom peptides in association with standard drugs: a novel strategy for combating antibiotic resistance - an overview

description: Abstract Development of antibiotic resistance that leads to resurgence of bacterial infections poses a threat to disease-free existence for humankind and is a challenge for the welfare of the society at large. Despite research efforts directed towards treatment of pathogens, antibiotics within new improved classes have not emerged for years, a fact largely attributable to the pharmacological necessities compelling drug development. Recent reversion to the use of natural products alone or in combination with standard drugs has opened up new vistas for alternative therapeutics. The success of this strategy is evident in the sudden interest in plant extracts as additives/synergists for treatment of maladies caused by drug-resistant bacterial strains. Animal venoms have long fascinated scientists as sources of pharmacologically active components that can be exploited for the treatment of specific ailments and should be promoted further to clinical trials. In the present review, we outline the scope and possible methods for the applications of animal venoms in combination with commercial antibiotics to offer a better treatment approach against antibiotic-resistant infections.
DOI: 10.22008/fk2/oiajvo/6bbwjr
title: diagnostic_retrieval.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.22008/fk2/oiajvo/nx8pbk
title: grain_diameter.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287357.v1
title: Chronic venous ulcers: a review on treatment with fibrin sealant and prognostic advances using proteomic strategies

description: Abstract Venous ulcers are the main causes of chronic lower-limb ulcers. The healing difficulties encourage the research and development of new products in order to achieve better therapeutic results. Fibrin sealant is one of these alternatives. Besides being a validated scaffold and drug delivery system, it possesses excellent healing properties. This review covered the last 25 years of the literature and showed that the fibrin sealant is used in various clinical situations to promote the healing of different types of ulcers, especially chronic ones. These are mostly venous in origin and usually does not respond to conventional treatment. Commercially, only the homologous fibrin sealants obtained from human blood are available, which are highly efficient but very expensive. The heterologous fibrin sealant is a non-commercial experimental low-cost product and easily produced due to the abundance of raw material. The phase I/II clinical trial is already completed and showed that the product is safe and promisingly efficacious for the treatment of chronic venous ulcers. In addition, clinical proteomic strategies to assess disease prognosis have been increasingly used. By analyzing liquid samples from the wounds through proteomic strategies, it is possible to predict before treatment which ulcers will evolve favorably and which ones will be difficult to heal. This prognosis is only possible by evaluating the expression of isolated proteins in exudates and analysis using label-free strategies for shotgun. Multicentric clinical trials will be required to evaluate the efficacy of fibrin sealant to treat chronic ulcers, as well as to validate the proteomic strategies to assess prognosis.
DOI: 10.22008/fk2/oiajvo/vi6wxi
title: r0.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287354.v1
title: Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools

description: Abstract Zika virus (ZIKV), an emerging arthropod-borne virus (arbovirus) of the Flaviviridae family, is a current issue worldwide, particularly because of the congenital and neurological syndromes associated with infection by this virus. As the initial clinical symptoms of all diseases caused by this group are very similar, clinical diagnosis is difficult. Furthermore, laboratory diagnostic efforts have failed to identify specific and accurate tests for each virus of the Flaviviridae family due to the cross-reactivity of these viruses in serum samples. This situation has resulted in underreporting of the diseases caused by flaviviruses. However, many companies developed commercial diagnostic tests after the recent ZIKV outbreak. Moreover, health regulatory agencies have approved different commercial tests to extend the monitoring of ZIKV infections. Considering that a specific and sensitive diagnostic method for estimating risk and evaluating ZIKV propagation is still needed, this review aims to provide an update of the main commercially approved serological diagnostics test by the US Food and Drug Administration (FDA) and Brazilian National Health Surveillance Agency (ANVISA). Additionally, we present the technologies used for monoclonal antibody production as a tool for the development of diagnostic tests and applications of these antibodies in detecting ZIKV infections worldwide.
DOI: 10.22008/fk2/oiajvo/wxxxve
title: snow_specific_area.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287352.v1
title: Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line

description: Abstract Background Venoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not been extensively studied and limited data is available on them. The present study aims to evaluate the antiproliferative and biochemical properties of the venom obtained from a species of marine stingray (Dasyatis sephen) on human cervical cancer cell line HeLa. Methods The antiproliferative effect of D. sephen venom was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation method along with assessment of changes in the enzymatic and non-enzymatic antioxidant status. We observed intracellular reactive oxygen species (ROS) levels by DCFH-DA method, mitochondrial membrane potential alterations by rhodamine 123 staining and apoptotic morphological changes by acridine orange/ethidium bromide dual staining method. Results D. sephen venom enhances lipid peroxidative markers such as thiobarbituric acid reactive substance, conjugated diene, and lipid hydroperoxide in HeLa cell lines. Stingray venom enhances the ROS levels, which is evidenced by the increased 2–7-diacetyl dichlorofluorescein fluorescence. Further, D. sephen venom treatment altered the mitochondrial membrane potential in HeLa cells. Additionally, we observed increased apoptotic morphological changes in D. sephen venom-treated groups. Conclusions Dasyatis sephen venom exhibits potent antiproliferative effect on HeLa cell line and upon further purification it could be a promising antiproliferative agent.
DOI: 10.22008/fk2/oiajvo/ydnbxo
title: al.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.22008/fk2/oiajvo/kyznif
title: albedo_bb_planar_sw.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287349.v1
title: Performance-based design method for energy efficient buildings

description: Abstract Progress in digital technology over the past decades has enabled the emergence of new methods and techniques for design development with an emphasis on energy efficiency. The aim of this paper was to propose a performative design method for office buildings associating parametric modelling, optimisation-based simulation, evaluation and classification of energy performance. In order to do that, the Rhinoceros/Grashopper, Opossum and Honeybee softwares were used, respectively. The digital design models by Oxman (2006) were used as reference to structure experiments from case studies of office buildings located in Vitória, ES, Brazil. The parameters of the INMETRO standard for Energy Efficiency Class for Commercial, Service and Public Buildings (INI-C) guided the evaluations. The methodresulted in a 31% improvement in the total consumption of primary energy and a 54% reduction in the demand for refrigeration in buildings. The estimate of the influencedemonstrated significant impacts from the alteration of the type of glass, percentage of façade opening and the configuration of shaders. The studyshowedthat,despite the particularities, there is potential for applying the method in the initial stages of design development.
DOI: 10.22008/fk2/oiajvo/pisyyu
title: diagnostic_retrieval.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287346.v1
title: Computational modelling of an earth-air heat exchanger (EAHE) adapted to the weather conditions of Belém, Brazil

description: Abstract The world’s demand for energy is constantly increasing,while, at the same time, the reserves of fossil fuels are decreasing and will not be enough to satisfy the global energy demand in the future. In addition, conventional heating and cooling systems consume most of the energy demand and considerably increase costs. Earth-airheat exchangers (EAHE) are a sustainable option that consumes less energy and has the potential to improve thermal conditions in buildings. Despite it being a simple and old technology, research into this type of technology is verylimited in Brazil. There is a lack of studies in the northern region of this country, particularly in the city of Belém do Pará. Hence, the goal of this researchstudy was to develop a three-dimensional numerical model of EAHE using ANSYS Fluent to evaluate the influence of operational and constructive parameters such as air flow velocity, length and diameter of the duct on the thermal performance of this system adapted to climatic conditions and soil characteristics of this city. The results obtainedlead to the conclusionthat this system can reduce the air temperature at the duct outlet up to 5.91 °C.
DOI: 10.22008/fk2/oiajvo/nghwlz
title: grain_diameter.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287345.v1
title: Numerical evaluation of the thermal performance of different configurations of soil-air heat exchangers

description: Abstract The use of soil as an energy source is aimed at reducingenergy consumption in buildings resulting from the use of heating, ventilation, and air conditioning equipment. Due to its high thermal capacity, soil acts as a thermal reservoir, heating or cooling the air inside buildings, depending on the localclimate conditions. In thisstudy, the finite volume method was used to analyse the thermal performance of this type of earth-airheat exchanger (EAHE). Initially, the effects of soil thermal properties, air velocity, and duct diameter on the thermal performance of the EAHE were investigated under steadystate, as it is a limit condition. In addition, two configurations for the duct distribution in the soil (two and four steps) were analysed. The resultsshowed that the best performance for the EAHE was obtained using saturated soil and operating with an air velocity of 2.5 m/s in a duct with a 0.10 m diameter, and that a 0.5 m distance between the duct’s center lines was the most appropriate. In addition, simulations were performed intransient state, applyingclimate conditions through sinusoidal functions.
DOI: 10.22008/fk2/oiajvo/q4owqh
title: r0.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287342.v1
title: Evaluation of the types of base in the adhesion of gypsum plasters coating

description: Abstract The use of gypsum plaster paste as an internal wall covering is one of the most commonly used techniques in Brazil, usually made in small thicknesses by manual application or by mechanical projection. This study evaluates, experimentally, the adhesion strength of coatings made with gypsum plaster paste, considering different substrates and application heights. Four types of blocks (ceramic and concrete blocks, sealing and structural blocks), two types of slice cuttings (superficial and even substrate) and three application heights (up to 0.6 m, between 0.6 m and 1.2 m, and above 1.2 m)wereused. The results indicated a strong influence of the base and depth of cut on the adhesion, but no influence of the application height on adherence was identified, due to the natural process of the application of the gypsum plaster coating.
DOI: 10.22008/fk2/oiajvo/dm0e39
title: snow_specific_area.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287340.v1
title: Evaluation of the flammability and natural aging of green polymer composites and vermiculite clay for potential use as building material

description: Abstract Green polyethylene and vermiculite clay composites were developed through the fusion intercalation technique with the aim of investigating their potential use as a building material. The composites were processed in a single screw extruder and molded in a hydraulic thermopress. For the purpose of this study, flame propagation resistance and the natural aging effects of green polyethylene and its composites were analyzed. The samples were exposed to the abiotic degradation test for 90 days with the aim of assessing the effects of climate conditions on the material’s morphology and mechanical properties. The flammability test demonstrated the catalytic effect of the clay, since for all composites evaluated, the flame propagation resistance was inferior to that of the pure green polymer. The effects of natural aging on the morphology of the materials were characterized by the transition of the polymeric matrix from a ductile aspect to a more fragile appearance and the emergence of voids and internal fractures Hence, the mechanical performance of the systems was directly affected, as a result of several types of abiotic degradation suffered by the samples.
DOI: 10.22008/fk2/oiajvo/8szu49
title: al.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287336.v1
title: Neuro-fuzzy model for energy prediction of different concrete dosages in buildings

description: Abstract Anincreasingprocess of urbanisation anda growing urban population heighten the need to understand the energy costsof the production of building materials. One of the most importanttoolsapplied to monitor the use of non-renewableenergyresources in the production of conventionalconcretes is energy input, intowhichfurther research is needed. In this study, an ANFIS (adaptive neuro-fuzzy inference system) hybrid model was developed to predict energy input in order to evaluate the energy demand required for each component of the production of conventional concrete (cement, water, fine aggregate and coarse aggregate) using 101 experimental dosages, 101 validation dosages and energy coefficients available in literature. The resultsshowedthatan adequate dosage can generate energy cost savingsof 24.77% in the production of concrete, while still maintaining the mechanical characteristics of compressive strength for conventional constructions.
DOI: 10.22008/fk2/oiajvo/hbeztl
title: albedo_bb_planar_sw.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.22008/fk2/oiajvo/drpzee
title: diagnostic_retrieval.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287328.v1
title: The quality of leisure spaces in contemporary urbanisation: the case of the suburs of Campinas, Brazil

description: Abstract The fast and unequal process of urbanisation in Brazil in the past fifty years has posed challenges to the implementation and management of leisure areas in low-income suburbs of cities. This paper aims to analyse and discuss how the public open spaces predicted in urban development projects in suburbanareas of the municipality of Campinas, in the state of São Paulo, were treated by the municipality between 1970 and 2010. In the case of spaces converted into leisure areas, the study sought to verify whethercertain attributes referenced in the literature were considered, analysing two territorial sections from two contrasting regions in terms of the socioeconomic configuration of their populations for subsequent comparison between them. The outcomes show that most of those public open spaces had not had any public interventions to transform them into leisure spaces. Moreover, there was a difference between the two regions in terms of the treatment given to the areas that received interventions. While in the northeastern region, predominantly occupied by a high-income population, the public leisure areas present less social accessibility and better landscape quality, while in the southeast, which is predominantly occupied by a low-income population, the leisure areas are more accessible and more cohesive, but are more ill-equipped and present lower landscaping quality.
DOI: 10.22008/fk2/oiajvo/0wsrev
title: grain_diameter.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.22008/fk2/oiajvo/ihixfz
title: r0.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14287327.v1
title: Self-levelling gypsum paste for use as a subfloor layer

description: Abstract The subfloor is a layer of the floor system designed to smooth, leveland absorb deformations. Conventionally, subfloorsare made of a low-productivity dry cement-based mortar. The use of a self-levelling gypsum-based material allows faster curing, lower cost, and smoothness of the surface, as well asa lower thickness. This researchstudy aimed to develop self-levelling gypsum pastes (GSL), with only beta gypsum as a binder, as well as to evaluate the influence of the superplasticizer additive based on polycarboxylate (PCE). A dosage study was carried out on pastes containing a water-to-gypsum ratio of 0.35; 0.40; 0.45; 0.475; 0.50, and 0.55 along with the superplasticizer additive content adjusted for self-levelling spreading. The pastes were characterized in the fresh state as to the setting times, spreading and heat of hydration time. In the hardened state for resistance to compression, traction in flexion, surface hardness and SEM. GSL pastes were obtained with a water-to-plaster ratio of 0.45 and 0.475, containing 3.5 and 2.0% PCE, respectively. The PCE increased the setting times, modified the crystalline structure of the hemihydrate, and reduced the interlocking of the crystals with the consequent decrease in the mechanical properties.
DOI: 10.22008/fk2/oiajvo/lgwje3
title: snow_specific_area.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.22008/fk2/oiajvo/iy5g2w
title: al.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4