DOI: 10.5061/dryad.64s2v
title: Data from: Testing the efficiency of nested barriers to dispersal in the Mediterranean high mountain plant Edraianthus graminifolius (Campanulaceae)

description: Due to strong spatial heterogeneity and limited Pleistocene glaciation,
 the Balkan Peninsula is a major European biodiversity hotspot.
 Surprisingly little, however, is known about patterns and processes of
 intraspecific diversification of its biota in general and of high altitude
 species in particular. A well-suited system to test hypotheses with
 respect to various isolating factors acting at different geographic scales
 and to explore full-range phylogeographic patterns on the Balkan Peninsula
 is Edraianthus graminifolius (Campanulaceae), distributed in the western
 Balkan mountain systems, the southwestern Carpathians and the Apennine
 Peninsula. To this end, we used a dense population sampling and employed
 Amplified Fragment Length Polymorphism (AFLP) markers and plastid DNA
 sequences supplemented by ecological niche modelling. The strongest splits
 were inferred to separate southern and northern Balkan populations from
 the central ones, from where range extension occurred to the Carpathians
 and, in more recent times, once or twice to the Apennine Peninsula. The
 three genetic groups in the western Balkan Peninsula were remarkably
 congruent among molecular markers suggesting that the barriers to gene
 flow acted over long time periods facilitating allopatric differentiation.
 Each main group of Balkan populations contained genetically and
 geographically distinct subgroups, which likely are the result of local
 refugia during warmer periods. Evidently, the topographically highly
 complex and during the last glacial maximum only locally glaciated Balkan
 Peninsula is a hotspot of species richness and endemism as well as a
 sanctuary of intraspecific genetic diversity, even if the underlying
 causes remain insufficiently understood.
description: Edraianthus
 graminifolius_AFLP data matrixAFLP data matrix of
 Edraianthus graminifoliusEdraianthus
 graminifolius_AFLPs.xlsxMaximum Clade
 Credibility tree of plastid sequences from
 Edraianthus_graminifoliusMaximum Clade
 Credibility tree of plastid sequences from Edraianthus_graminifolius
 (trnGUCC intron to trnGUCC-trnRUCU intergenic spacer, rpl32-trnLUAG
 intergenic spacer, and trnTUGU- trnLUAA-trnFGAA intergenic spacers)
 obtained with the software BEASTEdraianthus_graminifolius_MCC
 tree.outAlignment of plastid DNA
 sequences obtained from Edraianthus graminifolius with coded
 gapsAlignment of plastid DNA
 sequences (trnGUCC intron to trnGUCC-trnRUCU intergenic spacer,
 rpl32-trnLUAG intergenic spacer, and trnTUGU- trnLUAA-trnFGAA intergenic
 spacers) obtained from Edraianthus graminifolius with coded
 gapsEdraianthus_graminifolius_coded.fasUnmodified alignment of plastid DNA sequences from Edraianthus graminifolius (without gap coding)Unmodified alignment of plastid DNA sequences (trnGUCC intron to trnGUCC-trnRUCU intergenic spacer, rpl32-trnLUAG intergenic spacer, and trnTUGU- trnLUAA-trnFGAA intergenic spacers) from Edraianthus graminifolius (without gap coding)Edraianthus_graminifolius_uncoded.fasTable for translating population numbers used in the sequence alignments and the MCC treefile into the population numbers used in the paper, the AFLP matrix and in GenBankTable for translating population numbers used in the sequence alignments and the MCC treefile into the population numbers used in the paper, the AFLP matrix and in GenBankTranslation table for population numbers in alignments and treefile.txt