DOI: 10.5061/dryad.15534h9
title: Data from: Growth mode and carbon source impact the surfaceome dynamics of Lactobacillus rhamnosus GG

description: Bacterial biofilms have clear implications in disease and in food
 applications involving probiotics. Here, we show that switching the
 carbohydrate source from glucose to fructose increased the biofilm
 formation and the total surface-antigenicity of a well-known probiotic,
 Lactobacillus rhamnosus GG. Surfaceomes (all cell surface-associated
 proteins) of GG cells grown with glucose and fructose in planktonic and
 biofilm cultures were identified and compared, which indicated
 carbohydrate source-dependent variations, especially during biofilm
 growth. The most distinctive differences under these conditions were
 detected with several surface adhesins (e.g., MBF, SpaC pilus protein and
 penicillin-binding proteins), enzymes (glycoside hydrolases, PrsA, PrtP,
 PrtR and HtrA) and moonlighting proteins (glycolytic,
 transcription/translation and stress-associated proteins, r-proteins, tRNA
 synthetases, Clp family proteins, PepC, PepN and PepA). The abundance of
 several known adhesins and candidate moonlighters, including enzymes
 acting on casein-derived peptides (ClpP, PepC and PepN), increased in the
 biofilm cells grown on fructose, from which the surface-associated
 aminopeptidase activity mediated by PepC and PepN was further confirmed by
 an enzymatic assay. The mucus binding factor (MBF) was found most abundant
 in fructose grown biofilm cells whereas SpaC adhesin was identified
 specifically from planktonic cells growing on fructose. An additional
 indirect ELISA indicated both growth mode- and carbohydrate-dependent
 differences in abundance of SpaC, whereas the overall adherence of GG
 assessed with porcine mucus indicated that the carbon source and the
 growth mode affected mucus adhesion. The adherence of GG cells to mucus
 was almost completely inhibited by anti-SpaC antibodies regardless of
 growth mode and/or carbohydrate source, indicating the key role of the
 SpaCBA pilus in adherence under the tested conditions. Altogether, our
 results suggest that carbon source and growth mode coordinate mechanisms
 shaping the proteinaceous composition of GG cell surface, which
 potentially contributes to resistance, nutrient acquisition and cell-cell
 interactions under different conditions. In conclusion, the present study
 shows that different growth regimes and conditions can have a profound
 impact on the adherent and antigenic features of GG, thereby providing new
 information on how to gain additional benefits from this probiotic.
description: LC-MS/MS identification
 of all surface-associated proteins from biofilm cells grown on
 fructoseMascot search result as
 in MGF file format reporting peak lists of mass spectrometry data (lists
 of mass and intensity pairs) from MS/MS spectra.BF_I_F011836.mgfLC-MS/MS identification of all surface-associated proteins from biofilm cells grown on fructoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.BF_II_F011837.mgfLC-MS/MS identification of all surface-associated proteins from biofilm cells grown on fructoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.BF_III_F011839.mgfLC-MS/MS identification of all surface-associated proteins from biofilm cells grown on glucoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.BG_I_F011834.mgfLC-MS/MS identification of all surface-associated proteins from biofilm cells grown on glucoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.BG_II_F011840.mgfLC-MS/MS identification of all surface-associated proteins from biofilm cells grown on glucoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.BG_III_F011841.mgfLC-MS/MS identification of all surface-associated proteins from planktonic cells grown on fructoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.PF_I_F011835.mgfLC-MS/MS identification of all surface-associated proteins from planktonic cells grown on fructoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.PF_II_F011843.mgfLC-MS/MS identification of all surface-associated proteins from planktonic cells grown on fructoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.PF_III_F011845.mgfLC-MS/MS identification of all surface-associated proteins from planktonic cells grown on glucoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.PG_I_F011833.mgfLC-MS/MS identification of all surface-associated proteins from planktonic cells grown on glucoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.PG_II_F011844.mgfLC-MS/MS identification of all surface-associated proteins from planktonic cells grown on glucoseMascot search result as in MGF file format reporting peak lists of mass spectrometry data (lists of mass and intensity pairs) from MS/MS spectra.PG_III_F011846.mgf