DOI: 10.5061/dryad.hhmgqnkd4
title: Coordinates activities of retrosplenial ensembles during resting-state encode spatial landmarks. Part 1 of 2

description: The brain likely uses off-line periods to consolidate recent memories. One
 hypothesis holds that the hippocampal output provides a unique, global
 linking or 'index' code for each memory, and that this code is
 stored in the cortex in association with locally encoded attributes of
 each memory. Activation of the index code is hypothesized to evoke
 coordinated memory trace reactivation thus facilitating consolidation.
 Retrosplenial cortex (RSC) is a major recipient of hippocampal outflow and
 we have described populations of neurons there with sparse and orthogonal
 coding characteristics that resemble hippocampal 'place' cells,
 and whose expression depends on an intact hippocampus. Using two-photon
 Ca2+ imaging, we recorded ensembles of neurons in the RSC during periods
 of immobility before and after active running on a familiar linear
 treadmill track. Synchronous bursting of distinct groups of neurons
 occurred during rest both prior to and after running. In the second rest
 epoch, these patterns were associated with the locations of tactile
 landmarks and reward. Complementing established views on the functions of
 the RSC, our findings indicate that the structure is involved with
 processing landmark information during rest.
description: This dataset includes imaging data collected from three
 Thy1-GCaMP6s transgenic mice in the Retrosplenial Cortex (RSC). Imaging
 data were acquired using a Thorlabs Bergamo II multiphoton microscope.
 Tissue was excited by a Ti:Sapphire laser (Coherent) tuned to an
 excitatory wavelength of 920 nm. Beam focusing and light collection were
 achieved by a 16X water immersion objective (Nikon, NA 0.8, 80-120 mW
 output power measured at the sample). Beam-scanning was conducted by
 Galvo-Resonant mirrors. Emitted fluorescent lights were detected by a
 GaAsP photomultiplier tube (Hamamatsu) and digitized to a resolution of
 800x800 pixels at a sampling rate of 19 Hz. We collected an 835x835 um
 window over layers II-III of the agranular RSC at depths between 100 and
 200 um (imaging windows centred at -1.8 to -2.5 mm AP, 0.5 mm
 ML).
description: Dataset divided into two DOIs due to large file size. See Related
 Resources for a link to the additional part. The
 original size of the files was 560 GB. The files were archived using tar
 and compressed using pigz. The folder structure is as
 follows: 1. animal ID, 2. date of recording, 3. session number. In folder
 2 resides abf files generated from Clampex for individual recordings.
 Channel 1 contains frame pulses corresponding to the start and the end of
 rasterization of each individual recorded frame. Channels 2 and 3
 correspond to outputs A and B respectively of the optical rotary encoder
 used in detecting the position of the animal over the treadmill belt.
 Finally, channel 5 contains the readout for the delivery of reward, which
 also serves to signal the start of a new lap. In folder 3 are raw imaging
 files along with xml files, which hold the experimental parameters,
 generated by the ThorImage software. Part 1 of 2
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