cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/6055h-1bd91
title: Staphylococcus aureus_WT

description: T7SS
description: <strong>Tilt Series Date:</strong> 2017-06-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Tracy Palmer, University of Dundee. Data collected and processed by Ki Woo and Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> Media: TSA 500g (Oxoid TSA and TSB from Thermo Fisher. 10525943), TBS 500g (10137562)Dilute an overnight culture 1/100Incubate it for 4 hr (--> OD 2)Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-29-18/rawdata/</p>Saureus_WT_06290007.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-29-18/rawdata/Saureus_WT_06290007.mrc"
        > <i class="download icon"></i> Download </a></p>

        Saureus_WT_06290007.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-29-18/3dimage_85473/Saureus_WT_06290007.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.urbbxp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Xerochrysum bracteatum",
 "Country is Denmark",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048470-250811113504898/datasets/export for details.
DOI: 10.22002/g2611-qdy92
title: NR1769/R1-16 conjugation

description: Grid box YWC231 grid1NR1769/R1-16 conjugating with NR1769 skinny E. coli(mixed for 5 min prior to freezing)
description: <strong>Tilt Series Date:</strong> 2016-11-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
  ycw2016-11-16-7_slicer17160.jpg: F-pilus machine ycw2016-11-16-7_slicer17161.jpg: F-pilus machine ycw2016-11-16-7_slicer17162.jpg: F-pilus machine ycw2016-11-16-7_slicer17163.jpg: F-pilus machine ycw2016-11-16-7_slicer17164.jpg: F-pilus machine? ycw2016-11-16-7_slicer17165.jpg: F-pilus machine? ycw2016-11-16-7_slicer17166.jpg: F-pilus machine ycw2016-11-16-7_slicer17167.jpg: F-pilus machine ycw2016-11-16-7_slicer17786.jpg: non-piliated T4SS?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-11-16-7/3dimage_79739/</p>20161116_YWC231-1_20007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-11-16-7/3dimage_79739/20161116_YWC231-1_20007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20161116_YWC231-1_20007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-11-16-7/rawdata/20161116_YWC231-1_20007.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.qadqsx
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Xerochrysum bracteatum",
 "Country is Estonia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048471-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/w8c05-xt653
title: Caulobacter crescentus ftsE del

description: Caulobacter crescentus, ftsE, FtsZ, Long cell, PYE, Xylose induction
description: <strong>Tilt Series Date:</strong> 2016-09-04</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Goley ED at Johns Hopkins University School of Medicine</p>
<strong>Purification / Growth Conditions / Treatment:</strong> When OD600 reaches 0.30, 2 ml medium was spun down gently (3000 rpm for 3 min) and the resuspension was 100 ul for cryo-grid preparation.</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 190/A2. defocus: -12um. magnification: 22500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-09-04-32/rawdata/</p>square20016.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-09-04-32/rawdata/square20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        square20016.rec,  Reconstruction (Pixel Size 1.92 nm), 622.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-09-04-32/3dimage_78718/square20016.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/52ckt-cz644
title: H. pylori 26695 WT / AGS

description: Grid box YWC243
description: <strong>Tilt Series Date:</strong> 2017-02-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 10 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-27-38/rawdata/</p>20170227_YWC243_20014.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-27-38/rawdata/20170227_YWC243_20014.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170227_YWC243_20014_part61_2.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-27-38/3dimage_82634/20170227_YWC243_20014_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.s8rwjc
title: Occurrence Download

description: A dataset containing 15262 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorbus alnifolia"
 ]
}

 The dataset includes 15262 records from 231 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048469-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/7kt33-1jf75
title: V.cholerae

description: This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.
description: <strong>Tilt Series Date:</strong> 2017-01-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Yi-wei</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: -60, 60.         dosage: 160e/A2. defocus: -6um. magnification: 27500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-64/3dimage_80200/</p>sq240001_part121_4.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-64/3dimage_80200/sq240001_part121_4.rec"
        > <i class="download icon"></i> Download </a></p>

        sq240001.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-64/rawdata/sq240001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b50af-z0k76
title: lighlyfixed_wt_serumbottle

description: Raw data files of lighlyfixed_wt_serumbottle
description: <strong>Tilt Series Date:</strong> 2015-07-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown using serum bottle. This sample was lightly fixed with 2.5% glutaraldehyde, the very same sample that was sent for SEM. After mixing with glutaraldehyde in a tube, the sample was applied on grid and frozen.</p>
<strong>Sample Preparation:</strong> blotting conditions: 4 ul sample, blot force 6, blot time 3, drain time 1s</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-25/rawdata/</p>atlas1_fixedwt_serumbottle0025.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-25/rawdata/atlas1_fixedwt_serumbottle0025.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas1_fixedwt_serumbottle0025_part121_5.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/39wwc-taw85
title: Planktonic cell of Proteus

description: Planktonic cell, PG layer, Proteus mirabillis
description: <strong>Tilt Series Date:</strong> 2015-12-07</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Purification / Growth Conditions / Treatment:</strong> OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
<strong>Sample Preparation:</strong> OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-07-28/3dimage_71271/</p>atlas10028_part121_54.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-07-28/3dimage_71271/atlas10028_part121_54.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas10028.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-07-28/rawdata/atlas10028.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.djqhgq
title: Occurrence Download

description: A dataset containing 112 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Lolliguncula diomedeae"
 ]
}

 The dataset includes 112 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048473-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/4cnra-k0t97
title: M. xanthus knockout minor pilins

description: Grid box YWC130Strain SA6815
description: <strong>Tilt Series Date:</strong> 2015-04-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-15/rawdata/</p>20150426_YWC130_10021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-15/rawdata/20150426_YWC130_10021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150426_YWC130_10021_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-15/3dimage_54879/20150426_YWC130_10021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zxw1w-hyv17
title: Myxococcus xanthus SA6892

description: Strain SA6892 (minor pilin cluster 2+3 deletion, Δmxan1021-1017; Δmxan1369-1365).Grid box YWC222
description: <strong>Tilt Series Date:</strong> 2016-08-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-72/rawdata/</p>20160822_YWC222_10073.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-72/rawdata/20160822_YWC222_10073.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160822_YWC222_10073_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-72/3dimage_78398/20160822_YWC222_10073_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rybjvs
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Xerochrysum bracteatum",
 "Country is India",
 "HasCoordinate is true"
 ]
}

 The dataset includes 15 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048472-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/mgsxs-9mv88
title: lighlyfixed_wt_serumbottle

description: Raw data files of lighlyfixed_wt_serumbottle
description: <strong>Tilt Series Date:</strong> 2015-07-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown using serum bottle. This sample was lightly fixed with 2.5% glutaraldehyde, the very same sample that was sent for SEM. After mixing with glutaraldehyde in a tube, the sample was applied on grid and frozen.</p>
<strong>Sample Preparation:</strong> blotting conditions: 4 ul sample, blot force 6, blot time 3, drain time 1s</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-8/rawdata/</p>atlas1_fixedwt_serumbottle0008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        atlas1_fixedwt_serumbottle0008_part121_7.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-8/3dimage_58686/atlas1_fixedwt_serumbottle0008_part121_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0pfwy-bwj34
title: Proteus

description: Swarmer cell, long cell, Proteus mirabillis .  [added 2018-08-03 20:59:25] Proteus cell
description: <strong>Tilt Series Date:</strong> 2015-11-19</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33599x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university. Preparethe cell and design the research.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> This sample is prepared with the help of George Aucer, Ph. D student at Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Sample Preparation:</strong> OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-11-19-15/3dimage_71089/</p>atlas40001_part121_22.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
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        atlas40001.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
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