DOI: 10.5061/dryad.8931zcs17
title: Data from: No trout about it: Behavioural and transcriptional effects of long-term noise exposure in brook trout (Salvelinus fontinalis)

description: Exposure to acute noise sources can lead to negative behavioural outcomes
 and fitness deficits in fishes, but it is unknown if fish can habituate to
 chronic exposures. As underwater noise increases globally, understanding
 how long-term exposures can affect behavioural, morphological and
 transcriptional measures of stress in fish is critical. We tested
 responses in captive brook trout (Salvelinus fontinalis) immediately after
 acute exposure to a noise and after two weeks of chronic exposure.
 Behavioural tests quantified fish movements before, during, and after
 sound presentation, with morphological and transcriptional changes
 assessed through ears and whole brains samples respectively.Pre-control
 and pre-experimental brook trout exhibited increased swimming distance and
 velocity, but after the two-week exposure, post-experimental fish showed
 no response to noise while the post-control group remained responsive.
 Post-experimental fish showed significant differences in transcription
 levels of genes involved in neuroplastic and stress responses relative to
 the other groups. Together these results suggest that while fish may
 appear unresponsive to anthropogenic noises after chronic exposure, they
 still show significant changes at the transcriptional level with possible
 long-term effects.
description: # No trout about it: behavioural and transcriptional effects of long-term
 noise exposure in brook trout (Salvelinus fontinalis)
 [https://doi.org/10.5061/dryad.8931zcs17](https://doi.org/10.5061/dryad.8931zcs17) ## Description of the data and file structure This dataset includes raw data files for transcriptional, morphological and behaviour data for a study conducted on brook trout that were exposed to acute and chronic noise. Each file contains a “dictionary tab” defining variables found in the sheet. ### Files and variables #### File: BrookTroutAuditoryMorphologyData.xlsx **Description:** The file named “BrookTroutAuditoryMorphologyData” contains one sheet of data collected from the ears of fish. **Variables**  * Variable explanations for this data can be found on the “Dictionary” tab. #### File: BrookTroutBehaviourData.xlsx **Description:** The file named “BrookTroutBehaviourData” contains data from acute sound exposure trials for four behavioural response variable in fish before and after two-weeks of chronic sound exposure. Acute tests before the two-week period can be found in the “Pre” tab and acute tests following the two-week period can be found in the “Post” tab with combined data found in the “Combined” tab. ##### Variables * Variable explanations for this data can be found on the “Dictionary” tab. #### File: BrookTroutGeneTranscriptionData.xlsx **Description:** The file named “BrookTroutGeneTranscriptionData” contains relative gene expression data for 22 target genes each using values generated from an OpenArray nanofluidic chip qPCR. Calculations for each gene can be found on individual tabs within the document with gene name and function provided. The method used for relative transcription calculations is based off of Tuomi, 2010 (see full reference below) and was used to incorporate PCR efficiency when calculating transcription levels. Variable explanations for this data can be found on the “Dictionary” tab. Within the dictionary tab, formulas for calculating all variables are provided in the corresponding cell along with clarification regarding that variable. Additionally, clarification is provided regarding the layout of the gene tabs within the document. The gene tabs have been simplified for ease of use as only the “DCt” values are used for analysis. To perform these calculations, you will need Crt values provided by the OpenArray system as well as PCR efficiency values. All other variables within are calculated based on these two values. ##### Variables * Variable explanations for this data can be found on the “Dictionary” tab.