cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/s984x-kpp04
title: E. Coli delta53

description: Mutant MreB with sfGFP in sandwich position. WT MreB is replaced at the original locus with this fusion.
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia Coli </p>
<strong>Strain:</strong> delta53</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Josh Schaevitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 celsius to OD 600 ~ 0.3</p>
<strong>Sample Preparation:</strong> Blotted manually from rear in vitrobot at 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-01-42/rawdata/</p>040114_delta53_0004.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-01-42/rawdata/040114_delta53_0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        040114_delta53_0004.rec,  Reconstruction (Pixel Size 1.68 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-01-42/3dimage_41587/040114_delta53_0004.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xret0-p7364
title: Paeruginosa_wt_t24

description: wild type Pseudomonas aeruginosa
description: <strong>Tilt Series Date:</strong> 2014-02-09</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Lisa Racki</p>
<strong>Purification / Growth Conditions / Treatment:</strong> wild type starved for 24h</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-09-25/rawdata/</p>wt240001.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-09-25/rawdata/wt240001.mrc"
        > <i class="download icon"></i> Download </a></p>

        wt240001_part121_3.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-09-25/3dimage_49293/wt240001_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8k51n-4tx82
title: Pseudoalteromonas Iuteoviolacea MP129

description: P Iuteo DvipABhcpI Dbact2 whole cells from liquid culture
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab, taken at Janelia Farms</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid culture</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-39/rawdata/</p>2013_07_12_JF_MP129_b_0040.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-39/rawdata/2013_07_12_JF_MP129_b_0040.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_07_12_JF_MP129_b_0040_2k_secs_full.rec,  Reconstruction (Pixel Size 1.68 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-39/3dimage_28991/2013_07_12_JF_MP129_b_0040_2k_secs_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/htj5k-6h017
title: PAO1 Wild Type

description: Pseudomonas aeruginosa PAO1 Wild Type
description: <strong>Tilt Series Date:</strong> 2014-10-14</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-8/rawdata/</p>PAOIWTMCTest0021_mcorr.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-8/rawdata/PAOIWTMCTest0021_mcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        PAOIWTMCTest0021_mcorr_full.rec,  Reconstruction (Pixel Size 1.28 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-8/3dimage_47031/PAOIWTMCTest0021_mcorr_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wdpb5-0gs74
title: Methanotroph

description: Methanotroph_starved
description: <strong>Tilt Series Date:</strong> 2013-06-22</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Methyloprofundus sedimenti</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Victoria Orphan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> starved</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-22-34/rawdata/</p>methano_starved0003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-22-34/rawdata/methano_starved0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        methano_starved0003_part121_5.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-22-34/3dimage_32266/methano_starved0003_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pav9e-5qk37
title: AZ21

description: Caulobacter crescentus CB15N with inframe deletion of all three lytic transglycosylases
description: <strong>Tilt Series Date:</strong> 2013-09-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in PYE</p>
  aba2013-09-17-14_slicer6969.jpg: S-layer aba2013-09-17-14_slicer6971.jpg: S-layer aba2013-09-17-14_slicer6973.jpg: Unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-09-17-14/rawdata/</p>CaulobacterAZ2122mag0014.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-09-17-14/rawdata/CaulobacterAZ2122mag0014.mrc"
        > <i class="download icon"></i> Download </a></p>

        CaulobacterAZ2122mag0014_full.rec,  Reconstruction (Pixel Size 0.96 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-09-17-14/3dimage_31245/CaulobacterAZ2122mag0014_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.mku9jq
title: Occurrence Download

description: A dataset containing 1300113 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is Philippines",
 "OccurrenceStatus is Present",
 "Year 2000-2025"
 ]
}

 The dataset includes 1300113 records from 60 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007060-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/6dff2-54k14
title: Agrobacterium tumefaciens

description: Agrobacterium_VirB8-GFP
description: <strong>Tilt Series Date:</strong> 2013-05-07</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from PatZambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Titan_K2</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-70/rawdata/</p>VirB80020.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-70/rawdata/VirB80020.mrc"
        > <i class="download icon"></i> Download </a></p>

        VirB80020_part123_2.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-70/3dimage_32470/VirB80020_part123_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nt2j1-c2673
title: E. coli pED208

description: E. coli with plasmid pED208
description: <strong>Tilt Series Date:</strong> 2014-12-16</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells arrived dead.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-25/rawdata/</p>pED2080017.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-25/rawdata/pED2080017.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080017_part121_1.rec,  Reconstruction (Pixel Size 2.52 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-25/3dimage_48826/pED2080017_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7nsc4-c5244
title: Bsubtilis_ponA_sacculi

description: Bsubtilis_sacculi_ponA
description: <strong>Tilt Series Date:</strong> 2012-02-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-9/rawdata/</p>MJsacculi0014.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-9/rawdata/MJsacculi0014.mrc"
        > <i class="download icon"></i> Download </a></p>

        MJsacculi0014.rec,  Reconstruction (Pixel Size 1.9 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-9/3dimage_26215/MJsacculi0014.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4s3dc-bfd67
title: Agrobacterium tumefaciens

description: VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> AutoPatch</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB + antibiotics</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-34/rawdata/</p>atlas20003.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-34/rawdata/atlas20003.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas20003.rec,  Reconstruction (Pixel Size 1.6 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-34/3dimage_34256/atlas20003.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wb4w0-db850
title: Vibrio Stationary Phase

description: Vibrio cholerae, deep stationary phase, 37°C, pH 7.5
description: <strong>Tilt Series Date:</strong> 2014-01-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MKW1383 grown 28 hours in LB pH 7.5 at 37°C</p>
  aba2014-01-29-25_slicer10018.jpg: Vibrio cholerae .  pili aba2014-01-29-25_slicer10019.jpg: Vibrio cholerae .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-29-25/rawdata/</p>VibriostationaryLBmanualblot0025.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-29-25/rawdata/VibriostationaryLBmanualblot0025.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibriostationaryLBmanualblot0025_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-29-25/3dimage_38180/VibriostationaryLBmanualblot0025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bzkcj-1e733
title: E. coli pED208

description: E. coli with plasmid pED208
description: <strong>Tilt Series Date:</strong> 2014-12-16</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells arrived dead.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-19/rawdata/</p>pED2080011.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-19/rawdata/pED2080011.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080011_full.rec,  Reconstruction (Pixel Size 2.52 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-19/3dimage_48814/pED2080011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7f16j-9gx28
title: HeLa cells

description: Looking for ESCRT around midbodies
description: <strong>Tilt Series Date:</strong> 2014-11-26</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> Cells provided by Doug Mckarty</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA nup153+Thymidine 2mM for 14 h.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-17/rawdata/</p>midbody10001.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-17/rawdata/midbody10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody10001.rec,  Reconstruction (Pixel Size 2.4 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-17/3dimage_48370/midbody10001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g1bmx-ch551
title: PAO1 Wild Type

description: Pseudomonas aeruginosa PAO1 Wild Type
description: <strong>Tilt Series Date:</strong> 2014-10-14</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-14/rawdata/</p>PAOIWTMCTest0004.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
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        PAOIWTMCTest0004_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
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