DOI: 10.5061/dryad.bn006q0
title: Data from: The family placement of Cyrtandromoea

description: Cyrtandromoea is a genus consisting of about 12 species of perennial
 caulescent herbs with a distribution in China, India, Myanmar, Thailand,
 and western Malesia. The genus has previously been associated with either
 Gesneriaceae or the tribe Mimuleae in Phrymaceae (in former
 Scrophulariaceae) with morphology in favor of the latter and molecular
 plastid ndhF data the former. We addressed the placement of this genus by
 assembling a four gene dataset (matK, ndhF, rps16 and trnL-F) comprising
 270 ingroup samples representing 270 species and 51 families of the core
 asterids, including all families of Lamiales currently recognized. These
 included 111 species representing 66 genera of Gesneriaceae. We used
 maximum parsimony, maximum likelihood and Bayesian inference analyses to
 reconstruct phylogenies that showed Cyrtandromoea to be placed in
 Phrymaceae. A fine-scale analysis focusing on Phrymaceae using ITS and
 trnL-F and 79 samples revealed that Cyrtandromoea is most closely related
 to a clade of genera in Phrymaceae that included Mimulus s. s. A
 combination of morphological synapomorphies such as cymose inflorescences,
 5-angled toothed calyx, flowers with bilocular ovary with axile
 placentation with numerous small seeds with endosperm, and loculicidal
 dehiscing capsule support this placement. Our results strongly support the
 placement of Cyrtandromoea in Phrymaceae.
description: Figure S1, S2, and
 S3FIG
 S1: Holotype from L. of the generic type of Cyrtandromoea decurrens (Bl.)
 Zoll. (Blume 229) (Modified, from the Nationaal Herbarium Nederland,
 Naturalis Biodiversity Center). FIG S2: Illustration of generic type of
 Cyrtandromoea decurrens (Bl.) Zoll. (Modified, from the Peter H. Raven
 Library/Missouri Botanical Garden). FIG S3: Field images of Cyrtandromoea
 grandiflora C.B.Clarke (M. Möller et al. 06-966). A: Suffrutescent habit;
 B: Tubular toothed calyx and bilamellate stigma; C: Front view of mature
 flower: D: Lateral view of mature flower; E: Flower opened longitudinally
 showing four stamens and presence of two prominent yellow ridges on
 ventral surface of corolla tube. Scale bars: A: 10 cm, B-E: 1 cm.
 (Photographs by M.Möller).Dryad_Fig_S1-S3_13Mrch2019.pdfDryad_Fig_S4_MP_12Oct2018Fig. S4: Maximum Parsimony majority rule consensus tree of 11136 trees (37259 steps; CI=0.2942; RI=0.6383) based on a concatenated matrix of four chloroplast sequences on 281 samples across Lamiids. Numbers along the branches are majority rule frequencies.Dryad_Fig_S5_ML_16Feb2019Fig. S5: Best Maximum Likelihood tree on concatenated matrices of four chloroplast sequences on 281 samples across Lamiids. Numbers along the branches are ML bootstrap values. Hyphens (-) indicate branches with &lt;50% support.Dryad_Fig_S6_BI_16Feb2019Fig. S6: Bayesian Inference majority rule consensus tree based on concatenated matrices of four chloroplast sequences on 281 samples across Lamiids. Numbers along branches are posterior probabilities. Hyphens (-) indicate branches with &lt;0.5 support.Dryad_Fig_S7-small_MP_16Feb2019FIG. S7: Maximum parsimony strict consensus tree of 8 trees (3372 steps; CI=0.4389; RI=0.7558) on combined ITS and trnL-F data for 79 samples, with bootstrap values above the branches. Hyphen (-) indicates values &lt;50%.Dryad_Fig_S8-small_ML_16Feb2019FIG. S8: Maximum likelihood tree on combined ITS and trnL-F data for 79 samples with bootstrap values along the branches.Dryad_Appendix S1 BI_characteristics_large-scaleAPPENDIX S1: Characteristics of the Bayesian Inference run on a concatenated matrix of four chloroplast sequences on 281 samples across Lamiids.Dryad_Appendix S2 BI_characteristics_fine-scaleAPPENDIX S2: Characteristics of the Bayesian Inference run on a concatenated matrix of ITS and trnL-F on 79 samples in the fine-scale analysis.