DOI: 10.5061/dryad.pq6hg
title: Data from: Estimating exotic gene flow into native pine stands: zygotic versus gametic components

description: Monitoring contemporary gene flow from widespread exotic plantations is
 becoming an important problem in forest conservation genetics. In plants,
 where both seed and pollen disperse, three components of exotic gene flow
 with potentially unequal consequences should be, but have not been,
 explicitly distinguished: zygotic, male gametic and female gametic.
 Building on a previous model for estimating contemporary rates of zygotic
 and male gametic gene flow among plant populations, we present here an
 approach that additionally estimates the third (female gametic) gene flow
 component, based on a combination of uni- and biparentally inherited
 markers. Using this method and a combined set of chloroplast and nuclear
 microsatellites, we estimate gene flow rates from exotic plantations into
 two Iberian relict stands of maritime pine (Pinus pinaster) and Scots pine
 (P. sylvestris). Results show neither zygotic nor female gametic gene flow
 but moderate (6–8%) male gametic introgression for both species, implying
 significant dispersal of pollen, but not of seeds, from exotic plantations
 into native stands shortly after introduced trees reached reproductive
 maturity. Numerical simulation results suggest that the model yields
 reasonably accurate estimates for our empirical data sets, especially for
 larger samples. We discuss conservation management implications of
 observed levels of exposure to non-local genes, and identify research
 needs to determine potentially associated hazards. Our approach should be
 useful for plant ecologists and ecosystem managers interested in the
 vectors of contemporary genetic connectivity among discrete plant
 populations.
description: Nuclear and chloroplast
 microsatellite genotypes for Pinus pinaster at Fuencaliente (Ciudad Real,
 Spain)One line per combined
 nuclear-chloroplast individual genotype, comprising 12 nSSR diploid loci
 and 6 cpSSR haploid regions. For nSSR, the two alleles at each locus are
 listed in consecutive columns. Missing data coded with zeros. Needle
 samples collected in the field. DNA extracted with Invisorb DNA Plant HTS
 96 Kit. Electrophoresis of amplified products with LI-COR 4300 DNA
 Analyzer for cpSSRs, and 3130xL and 3500 Genetic Analyzers (Applied
 Biosystems) for nSSRs. Fragment size determination through visual
 inspection using external size markers and reference samples with known
 allele size for cpSSR, and internal size standard and GeneMapper software
 (Applied Biosystems) for nSSR.Pinus_pinaster_Fuencaliente_cp&nSSRs.csvNuclear and chloroplast microsatellite genotypes for Pinus sylvestris at Sierra Nevada (Granada, Spain)One line per combined nuclear-chloroplast individual genotype, comprising 10 nSSR diploid loci and 7 cpSSR haploid regions. For nSSR, the two alleles at each locus are listed in consecutive columns. Missing data coded with zeros. Needle samples collected in the field. DNA extracted with Invisorb DNA Plant HTS 96 Kit. Electrophoresis of amplified products with LI-COR 4300 DNA Analyzer for cpSSRs, and 3130xL and 3500 Genetic Analyzers (Applied Biosystems) for nSSRs. Fragment size determination through visual inspection using external size markers and reference samples with known allele size for cpSSR, and internal size standard and GeneMapper software (Applied Biosystems) for nSSR.Pinus_sylvestris_Sierra_Nevada_cp&nSSRs.csv