cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/p2pc8-bf953
title: Legionella delta DotOB

description: DotF-GFP background
description: <strong>Tilt Series Date:</strong> 2019-02-13</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph Vogel</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase</p>
<strong>Sample Preparation:</strong> 10 nm gold beads, broad vitrobot, R2/2 200 mesh copper quantifoil grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-55/rawdata/</p>20190213_deltaOB_59.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-55/rawdata/20190213_deltaOB_59.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190213_deltaOB_59_full.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-55/3dimage_101495/20190213_deltaOB_59_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2zauzv
title: Occurrence Download

description: A dataset containing 3318 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Bromus secalinus hirsutus, Bromus secalinus hirtus, Bromus secalinus hirtus, Bromus secalinus hordeaceus, Bromus secalinus hordeaceus, Bromus secalinus hybridus, Bromus secalinus hybridus, Bromus secalinus integer, Bromus secalinus intermedius, Bromus secalinus lasiophyllus, Bromus secalinus leiophyllus, Bromus secalinus macrostachys, Bromus secalinus minimus, Bromus secalinus muticus, Bromus secalinus muticus, Bromus secalinus polyanthus, Bromus secalinus pratensis, Bromus secalinus pubescens, Bromus secalinus pubescens, Bromus secalinus rhenanthus, Bromus secalinus secalinus, Bromus secalinus submuticus, Bromus secalinus submuticus, Bromus secalinus submuticus, Bromus secalinus vitiosus, Bromus secalinus vulgaris, Bromus segetalis, Bromus segetalis, Bromus segetalis, Bromus segetalis secalinus, Bromus segetalis secalinus, Bromus submuticus, Bromus vitiosus, Festuca secalina, Forasaccus secalinus, Serrafalcus divaricatus, Serrafalcus gmelinii, Serrafalcus secalinus, Serrafalcus secalinus elongatus, Serrafalcus secalinus glaber, Serrafalcus secalinus hordeaceus, Serrafalcus secalinus submuticus, Serrafalcus secalinus submuticus, Serrafalcus secalinus submuticus, Calamagrostis stricta, Agrostis arundinacea, Agrostis arundinacea contracta, Arundo groenlandica, Arundo stricta, Arundo stricta)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen, Machine Observation)"
 ]
 }
 },
 "Continent is Europe",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2024"
 ]
}

 The dataset includes 3318 records from 104 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047398-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ad42y-txf13
title: Nitrosocaldus yellowstonensis

description: Raw data files of Nitrosocaldus yellowstonensis
description: <strong>Tilt Series Date:</strong> 2019-07-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Candidatus Nitrosocaldus yellowstonensis</p>
<strong>Strain:</strong> HL72</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Jose R De La Torre and students, SF state, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R0.6/1 copper 300 mesh, 10nm gold, 22deg C, 100% humidity, manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-10/rawdata/</p>20190715_Ny_stationary_15.mrc,  Tilt Series (Pixel Size 0.2678 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-10/rawdata/20190715_Ny_stationary_15.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190715_Ny_stationary_15.mrc.mdoc,  31.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-07-15-10/rawdata/20190715_Ny_stationary_15.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/as18m-cxx94
title: Caulobacter delta-CpaF

description: Tad pilus system
description: <strong>Tilt Series Date:</strong> 2019-02-15</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> AM369, ΔldpF</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 193.8 eV/Å²,  defocus: -6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> iMOD</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier who provided WT and mutants</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 28 degrees Celsius</p>
<strong>Sample Preparation:</strong> plunge-freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-15-1/rawdata/</p>stalk_2.mrc,  Tilt Series (Pixel Size 0.351 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-15-1/rawdata/stalk_2.mrc"
        > <i class="download icon"></i> Download </a></p>

        stalk_2_full.rec,  Reconstruction (Pixel Size 1.404 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-15-1/3dimage_101567/stalk_2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a6ayj-tnj58
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-11-14</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, WT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-10/rawdata/</p>targ10.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-10/rawdata/targ10.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ10_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-10/3dimage_99686/targ10_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zgefn-yv793
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-146/rawdata/</p>caulobacter_na1000_wt_147_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-146/rawdata/caulobacter_na1000_wt_147_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_147_af.mrc.mdoc,  812.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-146/rawdata/caulobacter_na1000_wt_147_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q96ce-py694
title: gh9-3_sq3_cell4

description: GH9-3 ; sq3 ; 1.2s
description: <strong>Tilt Series Date:</strong> 2018-10-10</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight 6mL culture - Shaken, centrif 2500rcf 10min 30C, resuspended in 0.5mL SH medium.</p>
<strong>Sample Preparation:</strong> +13min post-prep' - 30C, 100% hum Manual backblot 5s FM layed on grid prior and backblotted to dry excess liquid.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-10-10-2/rawdata/</p>gh9-3_sq3_cell4.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-10-10-2/rawdata/gh9-3_sq3_cell4.mrc"
        > <i class="download icon"></i> Download </a></p>

        gh9-3_sq3_cell4_full.rec,  Reconstruction (Pixel Size 0.96 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-10-10-2/3dimage_97583/gh9-3_sq3_cell4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1xnkf-0v888
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-17</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-13/rawdata/</p>targ11.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-13/rawdata/targ11.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ11_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-13/3dimage_99547/targ11_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m0apm-ekf81
title: P. gingivalis PorV mutant

description: Raw data files of P. gingivalis PorV mutant
description: <strong>Tilt Series Date:</strong> 2019-01-23</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Reynolds</p>
<strong>Sample Preparation:</strong> Liquid culture. Harvested and plunge-frozen in late phase.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2019-01-23-47/rawdata/</p>2-PorV-52.mrc,  Tilt Series (Pixel Size 0.3519 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2019-01-23-47/rawdata/2-PorV-52.mrc"
        > <i class="download icon"></i> Download </a></p>

        2-PorV-52_full.rec,  Reconstruction (Pixel Size 1.4076 nm), 267.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2019-01-23-47/3dimage_100591/2-PorV-52_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ybvkbu
title: Occurrence Download

description: A dataset containing 498493859 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Continent is North America",
 "GadmGid is USA",
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Viperidae, Procyonidae, Canidae, Viverridae, Mustelidae, Mephitidae, Herpestidae, Cynomys, Mustela nigripes, Peromyscus, Salamandra, Sus scrofa, Colubridae, Anseriformes, Passeriformes)",
 "Year 2015-2025"
 ]
}

 The dataset includes 498493859 records from 38 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047313-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/9fnvk-ng070
title: JV9264

description: Raw data files of JV9264
description: <strong>Tilt Series Date:</strong> 2018-09-06</p>
<strong>Data Taken By:</strong> Jun Zhang</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degree and OD 2.5-3.0</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-28/rawdata/</p>JV9264_B124.mrc,  Tilt Series (Pixel Size 0.2654 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-28/rawdata/JV9264_B124.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9264_B124_part55_14.rec,  Reconstruction (Pixel Size 1.0616 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-28/3dimage_97793/JV9264_B124_part55_14.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.tcw6kp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pinus radiata",
 "Country is Uruguay",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047399-250811113504898/datasets/export for details.
DOI: 10.22002/0dxnq-h2a74
title: JV9264

description: Raw data files of JV9264
description: <strong>Tilt Series Date:</strong> 2018-09-10</p>
<strong>Data Taken By:</strong> Jun Zhang</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -14.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degree and OD 2.5-3.0</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-10-56/rawdata/</p>JV9264_TS60.mrc,  Tilt Series (Pixel Size 0.2654 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-10-56/rawdata/JV9264_TS60.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9264_TS60_part55_9.rec,  Reconstruction (Pixel Size 1.0616 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-10-56/3dimage_98190/JV9264_TS60_part55_9.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0acye-97c43
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-111/rawdata/</p>caulobacter_na1000_wt_112_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-111/rawdata/caulobacter_na1000_wt_112_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_112_af.mrc.mdoc,  814.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-111/rawdata/caulobacter_na1000_wt_112_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jeh5e-ngm23
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-96/rawdata/</p>caulobacter_na1000_wt_097_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
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        caulobacter_na1000_wt_097_af.mrc.mdoc,  812.0 B  
        
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