cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15156/bio/sh0192682.10fu
title: SH0192682.10FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh0192683.10fu
title: SH0192683.10FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh0192684.10fu
title: SH0192684.10FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh0192685.10fu
title: SH0192685.10FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15468/dl.vxpsqy
title: Occurrence Download

description: A dataset containing 1657 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ludwigia longifolia (DC.) H.Hara"
 ]
}

 The dataset includes 1657 records from 141 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019472-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.sjeqqn
title: Occurrence Download

description: A dataset containing 211616 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Aix galericulata (Linnaeus, 1758)"
 ]
}

 The dataset includes 211616 records from 696 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019442-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.56pqx8
title: Occurrence Download

description: A dataset containing 3989760 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((9.26588 63.69613,9.50615 63.39286,9.86654 63.20092,10.28033 63.17081,10.94773 63.35697,11.38822 63.57164,11.66853 63.76702,11.68187 64.00204,11.495 64.17702,11.13461 64.22349,10.70747 64.14211,9.33262 63.82596,9.26588 63.69613))",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 3989760 records from 331 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019439-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.4121/ca47907c-5c20-4c15-8460-1ec0fd9bb11f.v2
title: Data and codes underlying the publication: Tuning the Q-factor of Nanomechanical String Resonators by Torsion Support Design

description: Data and codes about the 1st paper "Tuning the Q-factor of Nanomechanical String Resonators by Torsion Support Design", which including the codes for analytical derivation, the COMSOL model for simulation and the experimental results. The contents are organized based on the number of figures in the published paper.
DOI: 10.4121/eb42378a-106a-4321-9cca-d1bc7bc4e51b.v2
title: Data and codes underlying the publication: Engineering Internal Resonance in Nanomechanical String Resonators by Soft-Clamping Supports

description: Data and codes about the 4th paper "Engineering Internal Resonance in Nanomechanical String Resonators by Soft-Clamping Supports", which including the codes for analytical derivation, the COMSOL model for simulation and the experimental results. The contents are organized based on the number of figures in the published paper.
DOI: 10.4121/f62aac24-e91b-49cb-8630-e98b621a7582.v2
title: Data and codes underlying the publication: Strain Engineering of Nonlinear Nanoresonators from Hardening to Softening

description: Data and codes about the 2nd paper "Strain engineering of nonlinear nanoresonators from hardening to softening", which including the codes for analytical derivation, the COMSOL model for simulation and the experimental results. The contents are organized based on the number of figures in the published paper.
DOI: 10.4121/66610544-dad6-48c1-a7fe-eb7263ce541a.v2
title: Data and codes underlying the publication: Finite Element-based Nonlinear Dynamic Optimization of Nanomechanical Resonators

description: Data and codes about the 3rd paper "Finite Element-based Nonlinear Dynamic Optimization of Nanomechanical Resonators", which including the codes for analytical derivation, the COMSOL model for simulation and the experimental results. The contents are organized based on the number of figures in the published paper.
DOI: 10.5281/zenodo.10473492
title: Tree symbol locations on historical 1:500 scale maps of Leeds and Edinburgh

description: These files contain the results of object detection on historical 1:500 scale Ordnance Survey maps of Leeds and Edinburgh.

The columns in the files are:



score - the score (confidence) of the detection.

class - if the detection is classed as a tree (broadleaf) or conifer.

area - the area of the detected bounding box.

size - the size label applied to the detection.

lat - latitude of tree location (WGS 84).

lon - longitude of tree location (WGS 84).

lat_bng - y value of location in British National Grid (OSGB36).

lon_bng - x value of location in British National Grid (OSGB36).
DOI: 10.5281/zenodo.10473493
title: Tree symbol locations on historical 1:500 scale maps of Leeds and Edinburgh

description: These files contain the results of object detection on historical 1:500 scale Ordnance Survey maps of Leeds and Edinburgh.

The columns in the files are:



score - the score (confidence) of the detection.

class - if the detection is classed as a tree (broadleaf) or conifer.

area - the area of the detected bounding box.

size - the size label applied to the detection.

lat - latitude of tree location (WGS 84).

lon - longitude of tree location (WGS 84).

lat_bng - y value of location in British National Grid (OSGB36).

lon_bng - x value of location in British National Grid (OSGB36).
DOI: 10.5281/zenodo.14175353
title: 2023 HYDRAS Proof of concept experiment with soybean genotypes
title: 2023_POC

description: Description

This data sets contains metadata and data of the 2023_POC experiment in the HYDRAS facility.

 The 2023 proof-of-concept study in hydras tested all standard field-phenotyping measurement types available in the infrastructure with 3 contrasting varieties of soybean + a control and a drought treatment using rain-out shelters. Start date: 23/5/2023, End dat: 4/10/2023. Location: Melle, Belgium. 

Data set contains: UAV sensor data, Electrical Resistivity Tomography data, soil point sensor data (water content, water potential and temperature), weather data, yield data and associated experimental information. 

Content



2023_POC_metadata.xlsx contains all information about the experiment (goal, design, sensors, treatments, biological material, ...) and about the associated data files.

Subfolder SPATIAL_INFO contains all spatial information about the experimental layout (location of field, plots, sensors, transects, ...) in GEOJSON files

Other subfolders contain the actual data from various sources as .csv files.
DOI: 10.15468/dl.arb53b
title: Occurrence Download

description: A dataset containing 4765 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ludwigia erecta (L.) H.Hara"
 ]
}

 The dataset includes 4765 records from 315 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019474-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.tep4dw
title: Occurrence Download

description: A dataset listing the 5447 species recorded in GBIF matching the query:
{
 "DatasetKey" : [
 "is Biowide Species data"
 ]
}

 The dataset's 5447 records were derived from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019446-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gsyhx4
title: Occurrence Download

description: A dataset containing 5904 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ludwigia hyssopifolia (G.Don) Exell"
 ]
}

 The dataset includes 5904 records from 295 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019477-241107131044228/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.tp2mqu
title: Occurrence Download

description: A dataset containing 2072 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sporobolus ×townsendii (H.Groves & J.Groves) P.M.Peterson & Saarela)",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Living Specimen, Machine Observation, Literature Occurrence)",
 "Geometry POLYGON((-180 -90,180 -90,180 90,-180 90,-180 -90))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1945"
 ]
}

 The dataset includes 2072 records from 59 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016619-250402121839773/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25919/9ahb-jt18
title: NBDL-HK3SZKWSMDPYZC
DOI: 10.25919/thft-mt77
title: NBDL-HK3SDG8RN3R0HP
DOI: 10.25919/901k-xe25
title: NBDL-HK4AMQXPNC88AJ
DOI: 10.25919/zz9z-a632
title: NBDL-HK3R7JRWK12X42
DOI: 10.25919/ckxm-e690
title: NBDL-HK4A8AF4HAHQVR
DOI: 10.15468/dl.78qrmd
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lagopus leucura",
 "Country is one of (Brunei Darussalam, Indonesia, Philippines, Timor-Leste, Singapore)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032796-250920141307145/datasets/export for details.
DOI: 10.25919/7fv1-m022
title: NBDL-HK3SXDV4G2J468
DOI: 10.25919/zpvq-bc20
title: NBDL-HK3RX9BJHZ7QW8
DOI: 10.25919/ds9g-9733
title: NBDL-JFE9FMT7KHMAS7
DOI: 10.25919/wfd5-zs38
title: NBDL-HK4A8AD9PAYA51
DOI: 10.25919/ng9t-4385
title: NBDL-HK4A8AFQKAG2AW
DOI: 10.25919/e023-v316
title: NBDL-HK3SXDWTK34AEZ
DOI: 10.25919/3y8m-4555
title: NBDL-HK3SPRSBJG4JVW
DOI: 10.25919/9vhv-et26
title: NBDL-HK3SXDXNN35E73
DOI: 10.25919/51sk-1d90
title: NBDL-HK4AMQZ4GCPQF7
DOI: 10.25919/p8ha-s282
title: NBDL-HK4A8AD8MAYCQX
DOI: 10.25919/s3yj-5t86
title: NBDL-HK3R0HMFP7V7NE
DOI: 10.25919/hq1t-1964
title: NBDL-HK3SXDW5N373N3
DOI: 10.34740/kaggle/dsv/13216030
title: Public Opinion on Climate Change (Updated Daily)
DOI: 10.25919/7wkc-bh36
title: NBDL-HK4B09H3PE6BEF
DOI: 10.15468/dl.ck8snd
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Parthenium argentatum",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 2 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032797-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25919/8bbw-pb69
title: NBDL-HK3RZTPCH4WJ2S
DOI: 10.25919/2ahg-vr54
title: NBDL-HK3SXDXMQ35DTQ
DOI: 10.25919/xd10-m015
title: NBDL-HK3QY5PZN9WW0Z
DOI: 10.25919/0gb6-k906
title: NBDL-HK3QATAXJPDX3Y
DOI: 10.25919/ztp9-m245
title: NBDL-HK48NMJCPSBA73
DOI: 10.25919/t769-kd41
title: NBDL-HK3SXDWNN34WK3
DOI: 10.25919/hdvc-c365
title: NBDL-HK3QD0SCGSAKQP
DOI: 10.25919/0r2n-2e18
title: NBDL-HK4B09HBPE7RVF
DOI: 10.15468/dl.8vfxkp
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Timonius alejandroanus"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032798-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25919/drqm-bj31
title: NBDL-HK3RX9C5JZMW7V
DOI: 10.25919/0hmc-n404
title: NBDL-HK4A8AFGHAG4Q8
DOI: 10.25919/6kg7-br43
title: NBDL-HK3SPRS6MG7AQG
DOI: 10.25919/rkbw-ja20
title: NBDL-HK4A8AF7KAHV0W
DOI: 10.25919/g4xc-3z42
title: NBDL-HK4B09HKPE6JGF
DOI: 10.25919/r4pt-t761
title: NBDL-HK4A8AFHQAG514
DOI: 10.25919/0azw-gq10
title: NBDL-HK4A8AEBKAGWKC
DOI: 10.25919/stzy-ev19
title: NBDL-HK3S2V8EJ8V8TG
DOI: 10.25919/6ttp-4905
title: NBDL-HK3RDJQZQ2EHK9
DOI: 10.25919/51gm-6d29
title: NBDL-HK3QATD0QP5ZQ5
DOI: 10.25919/qxgy-az97
title: NBDL-HK3QQM2GJ1QMGR
DOI: 10.25919/2x0y-7d31
title: NBDL-HK4AMQYZJCMM2B
DOI: 10.25919/j93a-dd15
title: NBDL-HK4A8AF8HAHAY8
DOI: 10.25919/hpaj-0y54
title: NBDL-HK48NMJBMSBCHZ
DOI: 10.25919/1bac-3y64
title: NBDL-HK4AMR07J9082G
DOI: 10.25919/asxp-8t65
title: NBDL-HK4A8ADGMAYZAX
DOI: 10.25919/xp7b-q726
title: NBDL-HK3SZKWHMDPHJC
DOI: 10.25919/dpmx-hf14
title: NBDL-HK48NMHYKSCGGY
DOI: 10.25919/yaqv-ns16
title: NBDL-HK3R0HKQN7YB4S
DOI: 10.25919/paez-c452
title: NBDL-HK3QATCQNP2TZH
DOI: 10.25919/c9dy-wa19
title: NBDL-HK4B09H2ME6DRV
DOI: 10.25919/2n0s-t461
title: NBDL-HK4B09G0GDSX33
DOI: 10.25919/zt34-4m50
title: NBDL-HK3R0HMJG7SH52
DOI: 10.25919/exdj-z387
title: NBDL-HK3RDJR4J2S3YE
DOI: 10.25919/cwkz-pr82
title: NBDL-HK3SXDV2M2J1B0
DOI: 10.25919/qn5f-4r55
title: NBDL-HXDQPBZRKNMT2S
DOI: 10.25919/zs5m-gq12
title: NBDL-HK4AMQXANC82K2
DOI: 10.25919/d0tz-xm19
title: NBDL-HK4AMQXZKC9P0E
DOI: 10.25919/j8f9-r602
title: NBDL-HK3SXDWVH34CVB
DOI: 10.25919/y72h-9a07
title: NBDL-HK4AMQZRGCPX6Q
DOI: 10.25919/fy1n-bj40
title: NBDL-HK4AMR03J902B0
DOI: 10.25919/v1n5-1k25
title: NBDL-HK4A8AEXQAKS4M
DOI: 10.25919/r3bh-ms10
title: NBDL-HK4A8AEGHAKH38
DOI: 10.25919/hhnb-bw46
title: NBDL-HK3SXDV9P2JKDM
DOI: 10.25919/qgq2-xf74
title: NBDL-HK4A8AFNQAGZFM
DOI: 10.25919/1qna-p196
title: NBDL-HK48NMG0QSF08P
DOI: 10.25919/48hx-bc53
title: NBDL-HK3SXDV1P2J0YM
DOI: 10.25919/4mxj-xf45
title: NBDL-HK4A8ADNPAY4KH
DOI: 10.15468/dl.hsgkv3
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lagopus muta",
 "Country is one of (Brunei Darussalam, Indonesia, Philippines, Timor-Leste, Singapore)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032800-250920141307145/datasets/export for details.
DOI: 10.15468/dl.jkvxmq
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Fallopia scandens",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032801-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25919/ek93-an78
title: NBDL-HK4B09GTMDREVV
DOI: 10.15468/dl.4pskzw
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Fallopia scandens",
 "Country is Philippines",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032802-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25919/tafn-c695
title: NBDL-HK3SXDXBH355HB
DOI: 10.25919/2waa-ng51
title: NBDL-HK4B09HXJE68K7
DOI: 10.25919/j63b-7x47
title: NBDL-HK4A8ADPGAY9RN
DOI: 10.57932/5c399263-a317-41b7-8900-184b177c4216
title: Ten years of measurements (2012-2022) of the atmospheric composition at Saclay/SIRTA Observatory in the Ile de France Region as part of ICOS and ACTRIS

description: This dataset includes measurements of CO₂, CO, CH₄, NOₓ, eBC, and O₃ collected by LSCE at the Saclay site, southwest of Paris. CO₂, CO, and CH₄ were measured at LSCE and on the Saclay tower, while NOₓ, eBC, and O₃ data were measured at LSCE. Wind data were also recorded on the Saclay tower. This dataset provides a comprehensive view of atmospheric composition and urban influence at a peri-urban site.
DOI: 10.25919/6616-cb57
title: NBDL-HK4A8ADAGAYFJ5
DOI: 10.25919/egwg-bx96
title: NBDL-HK3QQM29G1PGVM
DOI: 10.25919/r0fc-0n15
title: NBDL-HK3SXDXDN352T3
DOI: 10.25919/1xk4-kw89
title: NBDL-HK3S2V8QG8T4WC
DOI: 10.25919/j1zj-2d73
title: NBDL-HK3RZTQ4H4YMXS
DOI: 10.25919/q0a7-8z42
title: NBDL-HK3SXDX6K35G0F
DOI: 10.25919/5zyw-dt81
title: NBDL-HK4A8AG0MD6PR3
DOI: 10.25919/8erj-gh82
title: NBDL-HK3SPRQPQH3SAK
DOI: 10.25919/rsb9-tj64
title: NBDL-HK3SMQEWMSM9WV
DOI: 10.25919/yc2s-8008
title: NBDL-HK3SXDX9N358DK
DOI: 10.25919/4adp-f684
title: NBDL-HK3SZKV6HDS1AQ
DOI: 10.25919/z29a-a773
title: NBDL-HK3SZKWVGDPT04
DOI: 10.25919/s1wx-f745
title: NBDL-HK4B09FKQBJYKC
DOI: 10.25919/5esv-h943
title: NBDL-HK3SPRQRKH3W1V
DOI: 10.25919/11ch-fs54
title: NBDL-HK3QQM2HG1QN2M
DOI: 10.25919/c2n2-fm26
title: NBDL-HK3RDJSEP36CXP
DOI: 10.25919/gfx8-e887
title: NBDL-HK4A8AEFKAGPSW
DOI: 10.25919/vh7w-cd37
title: NBDL-HK4AMQY2MCQ0DF
DOI: 10.5517/ccdc.csd.cc2ks0p2
title: CCDC 2376574: Experimental Crystal Structure Determination

description: Related Article: Peiyao Pan|2024|CSD Communication|||
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.25919/p4wg-yz82
title: NBDL-HK3RX9DNJZQ39V
DOI: 10.5517/ccdc.csd.cc2ksdpg
title: CCDC 2376946: Experimental Crystal Structure Determination

description: Related Article: Peiyao Pan|2024|CSD Communication|||
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.25919/hejg-be43
title: NBDL-HK3SXDV6M2J72G
DOI: 10.25919/cbqs-nm35
title: NBDL-HK4A8AETNAK18G
DOI: 10.25919/fmtj-3q93
title: NBDL-HK4A8AEMHAJB9R
DOI: 10.25919/pjkr-5m93
title: NBDL-HK3SPRSAMG4G90
DOI: 10.5281/zenodo.17232862
title: WB uncropped blots & raw  raw densitometry

description: BCAA promotes protein synthesis by activating the Akt/mTORC1, which subsequently regulates its two major downstream effectors, ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Activated S6K1 subsequently inhibits eEF2K, preventing the inactivating phosphorylation of eEF2 and thus promoting translation elongation. Therefore, to explore the underlying signaling mechanism of the C2C12 myotube response to BCAA stimulation, we measured the protein levels of Akt, mTOR, p70S6K, 4E-BP1, and eEF2 by WB after 45 minutes of BCAA treatment. Moreover, the effects of BCAA on the phosphorylation of AMPK, Akt, p70S6K, and eEF2 after 24 hours of treatment were further investigated by conducting WB.

Method

Total protein was extracted with RIPA buffer from C2C12 myotubes and was then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The separated proteins were transferred to polyvinylidene difluoride membranes, which were blocked with 5% non-fat milk dissolved in Tris-buffered saline containing 0.05% Tween 20 (TBS-T) for 1 h at room temperature. Membranes were then incubated overnight at 4 °C with primary antibodies. Finally, membranes were incubated for 1 hour with the second antibodies, and visualized using an enhanced chemiluminescence system. Antibodies used in this study are listed in Table 2. Mean grey value and Integrated Density of each band were measured using ImageJ with Fiji (version 1.35). WB was performed twice for each sample, and statistical analysis was performed on data from three independent experiments (N = 3, n = 2).
DOI: 10.25919/y29a-hx08
title: NBDL-HK3QATCVNP57V1