cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/sqnsa-a1v72
title: HUVECs infx pNLGFP

description: Raw data files of HUVECs infx pNLGFP
description: <strong>Tilt Series Date:</strong> 2013-09-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 24h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-09-12-18/rawdata/</p>13sep12_HuvpNLgfp_24hPI_PI0002.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-09-12-18/rawdata/13sep12_HuvpNLgfp_24hPI_PI0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        13sep12_HuvpNLgfp_24hPI_PI0002_full.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-09-12-18/3dimage_30904/13sep12_HuvpNLgfp_24hPI_PI0002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tejg6-qvb56
title: HUVECs infx pNLBg1I

description: Raw data files of HUVECs infx pNLBg1I
description: <strong>Tilt Series Date:</strong> 2013-10-18</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen endpoint of virus infected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-18-9/rawdata/</p>13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0009.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-18-9/rawdata/13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0009_part81_1.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-18-9/3dimage_32849/13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0009_part81_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4s83j-j3f31
title: Agrobacterium VirD4-GFP 28C

description: Induction in liquid at 28C in AB+AS medium
description: <strong>Tilt Series Date:</strong> 2014-03-24</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Induction in liquid at 28C in AB+AS medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
  etl2014-03-24-4_slicer7430.jpg: Filament in Agrobacterium tumefaciens
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-24-4/rawdata/</p>VirD4_GFP_Clay0004.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-24-4/rawdata/VirD4_GFP_Clay0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        VirD4_GFP_Clay0004_full.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-24-4/3dimage_49421/VirD4_GFP_Clay0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bpjbt-zmn61
title: E.coli "strain 3" G235E CheA on

description: chromosomal chemoreceptor mutant G235E "CheA on"
description: <strong>Tilt Series Date:</strong> 2012-02-10</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sandy Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> culture grown in TB medium (+GEntamycin) to OD (600) 0.31 at 30 degrees. Additional one hour incubation with penicillin before freezing.</p>
<strong>Sample Preparation:</strong> freezing with Vitrobot @ 100 % humidity, -4 offset, 3.5 s blot time, no drain, 3 µl sample, pure ethane.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-12/rawdata/</p>strain30012.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-12/rawdata/strain30012.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain30012_part.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-02-10-12/3dimage_16360/strain30012_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vbe9j-a0w60
title: Agro VirB8-GFP

description: Raw data files of Agro VirB8-GFP
description: <strong>Tilt Series Date:</strong> 2013-10-03</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown in liquid at 19C and induced with 200uM AS for 24 hours</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-6/rawdata/</p>virb8_24h_2750007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-6/rawdata/virb8_24h_2750007.mrc"
        > <i class="download icon"></i> Download </a></p>

        virb8_24h_2750007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-6/3dimage_31824/virb8_24h_2750007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tmtjj-sfd71
title: Cryo-sectioned Jurkat

description: Jurkats were infected with pNLGFP virus then HP frozen and cryo-sectioned by Mark Ladinsky-Grid4
description: <strong>Tilt Series Date:</strong> 2012-03-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cryo-sectioning by Mark Ladinsky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-29-1/3dimage_69174/</p>12mar29_Jurkatcryosec_tgtsq7_AtlasBT2c_0001_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 103.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-29-1/3dimage_69174/12mar29_Jurkatcryosec_tgtsq7_AtlasBT2c_0001_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12mar29_Jurkatcryosec_tgtsq7_AtlasBT2c_0001_ns.st,  Tilt Series (Pixel Size 0.5 nm), 635.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-29-1/rawdata/12mar29_Jurkatcryosec_tgtsq7_AtlasBT2c_0001_ns.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wxw2s-4tb45
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-22/3dimage_68617/</p>12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0005_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-22/3dimage_68617/12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0005.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-22/rawdata/12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mqkpp-34k12
title: Agro C58 22C plate 16h

description: Raw data files of Agro C58 22C plate 16h
description: <strong>Tilt Series Date:</strong> 2014-11-10</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown at 22C and induced for T pili on a 2% agarose pad for 16 hours</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-20/rawdata/</p>c58_22_pl_16h20002.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-20/rawdata/c58_22_pl_16h20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_22_pl_16h20002_full.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-20/3dimage_49535/c58_22_pl_16h20002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c0y36-stt43
title: HUVECs infx pNLGFPBglI

description: Raw data files of HUVECs infx pNLGFPBglI
description: <strong>Tilt Series Date:</strong> 2013-06-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFPBglI obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen 24h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-06-14-1/rawdata/</p>13jun14_C160_2_HUVECspNLBglI24hPI_AT1BT1_0001.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-06-14-1/rawdata/13jun14_C160_2_HUVECspNLBglI24hPI_AT1BT1_0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jun14_C160_2_HUVECspNLBglI24hPI_AT1BT1_0001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-06-14-1/3dimage_27423/13jun14_C160_2_HUVECspNLBglI24hPI_AT1BT1_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ar2m5-2d088
title: Agro C58 22C plate 16h

description: Raw data files of Agro C58 22C plate 16h
description: <strong>Tilt Series Date:</strong> 2014-11-10</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown at 22C and induced for T pili on a 2% agarose pad for 16 hours</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-8/rawdata/</p>c58_22C_pl_16h0008.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-8/rawdata/c58_22C_pl_16h0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_22C_pl_16h0008_part121_10.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-8/3dimage_49598/c58_22C_pl_16h0008_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sttrx-axz89
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-34/3dimage_68641/</p>12apr28_C126_2_pNLDN_COS7_AT7BT1_0010_full.rec,  Reconstruction (Pixel Size 2.0 nm), 627.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-34/3dimage_68641/12apr28_C126_2_pNLDN_COS7_AT7BT1_0010_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT7BT1_0010.st,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-34/rawdata/12apr28_C126_2_pNLDN_COS7_AT7BT1_0010.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ysq44-kpk50
title: HUVECs infx pNLBg1I

description: Raw data files of HUVECs infx pNLBg1I
description: <strong>Tilt Series Date:</strong> 2013-10-19</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen endpoint of virus infected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-28/rawdata/</p>13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0013.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-28/rawdata/13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0013_part121_3.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-28/3dimage_32917/13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0013_part121_3.rec"
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DOI: 10.22002/kpd2t-60670
title: Agro Bald VirB8-GFP

description: the bald strain lacks flagellin 1-3 and is considered non-motile
description: <strong>Tilt Series Date:</strong> 2013-07-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown at 19C and induced for T4SS</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-21/rawdata/</p>bald_gfp20001.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
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        bald_gfp20001_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
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