DOI: 10.15482/usda.adc/1518738
title: Data from: Long-lasting insecticide-incorporated netting and interception traps at pilot-scale warehouses and commercial facilities prevents infestation by stored product beetles

description: Attraction Assessment - assessment of different lure sources, including pheromones and kairomones for red flour beetle and lesser grain borer in the wind tunnel and release-recapture experiment under controlled settings. Abbreviations: WGO - wheat germ oil; Tab - Insects Limited SPB tablet bait; NC - negative control (no stimulus); DDGS - dried distiller's grains with soluables. There were a total of n = 12 replicate releases for the release-recapture and n = 30 replicate individuals for the wind tunnel per treatment. Dose Dependency Data - evaluation of whether dose-dependency in attraction exists for red flour beetle and lesser grain borer in the wind tunnel and in a release-recapture experiment for the Insects Limited SPB lure. Abbreviations: SPB1 - a single Insects Limited SPB tablet bait; SPB2 - two Insects Limited SPB tablet baits; SPB3 - three Insects Limited SPB tablet baits; Ctrl - negative control (no stimulus); DDGS - dried distiller's grains with soluables. There were a total of n = 24 replicate releases for the release-recapture and n = 30 replicate individuals for the wind tunnel per treatment. Spillage Trap Collections - datasheet for the number and lowest taxonomic unit of insects collected in interception traps with different kill mechanisms and stimuli at three food facilities in Arkansas and Kansas during 2018 and 2019. There were a total of 27 taxa tracked, and captures totaled to near 4,000. Abbreviations: C, control netting only (no stimulus); L, insecticide-netting only (no stimulus); LS, insecticide-netting with a single SPB Insects Limited tab lure; CS, control netting with a single SPB Insects Limited tab lure. State abbreviations: AR - Arkansas, KS - Kansas. There were three transects per site, each with every treatment above represented, thus a total of n = 8-9 replicate deployments in AR and n = 12 deployments in KS. Spillage Trap Progeny- datasheet for progeny production in interception traps after six weeks under constant conditions with different kill mechanisms and stimuli at three food facilities in Arkansas and Kansas during 2018 and 2019. Abbreviations: C, control netting only (no stimulus); L, insecticide-netting only (no stimulus); LS, insecticide-netting with a single SPB Insects Limited tab lure; CS, control netting with a single SPB Insects Limited tab lure. State abbreviations: AR - Arkansas, KS - Kansas. There were three transects per site, each with every treatment above represented, thus a total of n = 8-9 replicate deployments in AR and n = 12 deployments in KS. Trial 1 Recapture - To understand whether the method by which LLIN was deployed affected subsequent commodity infestation and progeny production, pilot-scale warehouses (5.85 × 2.81 m) in Manhattan, KS were used. At the far end of the warehouse against the back wall, a commodity consisting of a mixture of 210 mL organic, whole wheat kernels and 210 mL of organic, unbleached flour was placed. A total of 100 individuals each of T. castaneum, R. dominica, and T. variabile were released at the opposite end of the warehouse (approx. 5.25 m away). There were n = 12 replicate releases per treatment from 26 April 2019 to 16 August 2019, comprising a total of 3,600 released insects. There were four LLIN deployment methods that were tested (Figure 2). In the “hanging” treatment, LLIN (2.72 × 2.41 m) was affixed to the warehouse ceiling and allowed to hang down to the floor, completely bisecting the room. In the “cover” deployment method, LLIN was directly laid over the commodity. In the “pipe” deployment method, a PVC pipe (91 cm length, 5.1 cm I.D.) was bisected halfway with LLIN. These were compared with a "control" that used the same PVC pipe design, but without netting. Insects were given 72 h to disperse across the warehouse to the commodity. After this period, insects were collected by pre-designated zones in the warehouse. The zones were noted respective to the location of the commodity, and included "in commodity" (inside the commodity), "partial dispersal" (0.5 m radius to 4.5 m away), and "no dispersal" (4.5 m–5.6 m away, e.g. the release zone). The insects were retrieved, and then brought back to the lab where their health condition was assessed as alive, affected, or dead. Abbreviations: RFB - red flour beetle; LGB - lesser grain borer; WHB - warehouse beetle. Trial 1 Progeny Production - as above but, the commodity was held for 6 weeks after deployment under constant environmental chamber conditions to evaluate progeny production. The species and health conditions of the progeny were recorded.Abbreviations: RFB - red flour beetle; LGB - lesser grain borer; WHB - warehouse beetle. Trial 2 Recapture - To understand whether management tactic affected subsequent commodity infestation and progeny production, pilot-scale warehouses (5.85 × 2.81 m) in Manhattan, KS were used. There were four treatments in total applied to warehouses for this experiment: LLIN alone (L), AK-based interception trap alone (AK), both together (LAK), or neither (e.g. "control" that had no netting or interception trap). The zones were similar to the descriptions above, but a Zone 7 was introduced which described insects captured inside the interception traps (e.g. "trap"). At the far end of the warehouse against the back wall, a commodity consisting of a mixture of 210 mL organic, whole wheat kernels and 210 mL of organic, unbleached flour was placed. A total of 100 individuals each of T. castaneum, R. dominica, and T. variabile were released at the opposite end of the warehouse (approx. 5.25 m away). There were n = 12 replicate releases per treatment from 23 August 2019 to 8 November 2019, comprising a total of 3,600 released insects. Insects were given 72 h to disperse across the warehouse to the commodity. After this period, insects were collected by pre-designated zones in the warehouse. The zones were noted respective to the location of the commodity, and included "in commodity" (inside the commodity), "partial dispersal" (0.5 m radius to 4.5 m away), and "no dispersal" (4.5 m–5.6 m away, e.g. the release zone). The insects were retrieved, and then brought back to the lab where their health condition was assessed as alive, affected, or dead. Abbreviations: RFB - red flour beetle; LGB - lesser grain borer; WHB - warehouse beetle. Trial 2 Progeny Production - as above but, the commodity was held for 6 weeks after deployment under constant environmental chamber conditions to evaluate progeny production. The species and health conditions of the progeny were recorded. Abbreviations: RFB - red flour beetle; LGB - lesser grain borer; WHB - warehouse beetle. This work was funded, in part, by a United States Department of Agriculture, National Institute of Food and Agriculture, Crop Protection and Pest Management Grant #2017-70006-27262.