DOI: 10.5061/dryad.j9kd51c8f
title: Data on assessing the effects of genetic divergence and morphology on Anolis lizard mating

description: The brown anole (Anolis sagrei) is a widespread neotropical lizard found
 on many islands in the West Indies as well as the coast of Central
 America. Across their range, brown anole populations show extensive
 ecomorphological trait variation and substantial genetic divergence. It is
 unclear, however, whether this genetic and morphological divergence
 results in reproductive isolation between populations. We investigated
 variation in mating behavior across populations by analyzing four hours of
 video of each of 234 captive male-female A. sagrei pairs encompassing all
 36 possible pairings from six sampled islands. For each pair of
 individuals, we tested for an association between the occurrence of
 mating, morphological traits, and genetic relatedness of their
 populations. We found no support for the hypotheses that ecological and/or
 non-ecological divergence is driving premating reproductive isolation in
 A. sagrei. We did find that males with relatively short heads tend to mate
 more quickly and hypothesize potential explanations that warrant future
 investigation.
description: <strong>For the matings_dryad
 dataset:</strong> Mating data: Adult <em>A.
 sagrei</em> were collected in the summer of 2016 from the Bahamian
 islands of Abaco, Bimini, Conception, and Staniel Cay and from the
 Caymanian islands of Cayman Brac and Little Cayman in the Cayman Islands.
 Female <em>A. sagrei</em>, which can store sperm from previous
 matings and store sperm in the absence of fertilization, were kept
 isolated from males until &lt;1% of eggs were fertile. Males were
 placed in individual 15-L cages and given over one month to acclimate, at
 which point one female was added to each cage and a GoPro was turned
 on. Mating, defined as cloacal alignment, occurs when the male is on the
 female’s dorsum and curves his tail underneath hers, toward her ventrum.
 Matings were recorded if they occurred in the first four hours.
 Skeletal data: Specimens were scanned using the INSPEX 20i
 digital x-ray system in the Harvard University Museum of Comparative
 Zoology’s Digital Imaging Facility. Heads and limbs were taped to the
 surface of the x-ray plate to control for foreshortening effects; if a
 limb is was not orthogonal to the x-rays and in the same plane as the
 scale bar, the captured image can misrepresent relative sizes between
 individuals. X-ray images were traced in ImageJ. Dewlap
 data: We photographed male dewlaps for a subset of the each population
 using a tripod-mounted Olympus EM-5mkII digital camera with a 60 mm macro
 lens. To determine surface area, dewlaps were traced from these
 photographs using the ImageJ plugin ObjectJ.
 <b>For the locations_dryad dataset:</b>
 Geographic coordinates of locations of collection were
 recorded. <b>For the island_sagrei_dryad.fasta
 dataset:</b> We sequenced NADH dehydrogenase
 subunit 2 (ND2) in five individuals from each of the six
 populations. Further details about data collection for
 all three datasets are available in the associated manuscript.
description: The dataset "matings_dryad" is a CSV file containing
 outcomes of four-hour-long mating trials of pairings of <em>Anolis
 sagrei </em>and morphological data about the individuals
 used. The dataset "locations_dryad" contains
 sites of collection of populations. The dataset
 "island_sagrei_dryad.fasta" is a multi-FASTA file containing ND2
 sequences from the individuals. The file
 "README_dryad" is a Word document that gives detail about these
 sequences. Missing morphological data in
 "matings_dryad" is due to some traits not being measured. All
 files are explained in the README_dryad file.