DOI: 10.5061/dryad.w6m905qz0
title: Data from: Repeated successful nest sharing and cooperation between Western Kingbirds (Tyrannus verticalis) and a female western kingbird X scissor-tailed flycatcher (T. forficatus) hybrid

description: Nest sharing by birds, or the phenomenon where multiple individuals of
 different species contribute genetically and parentally to a single nest,
 is a rare form of cooperative breeding that has only occasionally been
 reported in socially monogamous birds. Here we describe, both behaviorally
 and genetically, the unique case of two female birds, a western kingbird
 (Tyrannus verticalis) and a western kingbird x scissor-tailed flycatcher
 (T. forficatus) hybrid simultaneously occupying (and likely co-incubating)
 a brood in a single nest. Both females provisioned nestlings, and they did
 this in two consecutive years (producing four fledglings each year).
 Genomic data from the females revealed that they were unrelated and
 parentage analyses revealed that both females contributed genetically to
 at least one of the offspring, and at least two fathers were involved. In
 total, this is the first reported case of nest sharing involving a hybrid
 individual and the first case within the family Tyrannidae.
description: # Repeated Successful Nest Sharing and Cooperation between Western
 Kingbirds (Tyrannus verticalis) and a Female Western Kingbird x
 Scissor-tailed Flycatcher (T. forficatus) Hybrid
 [https://doi.org/10.5061/dryad.w6m905qz0](https://doi.org/10.5061/dryad.w6m905qz0) ## Description of the data and file structure To determine the relatedness of offspring from a shared nest that involved two western kingbirds and a female western kingbird x scissor-tailed flycatcher hybrid in Arkansas. ### Files and variables #### File: 5\_ind\_kingbird\_fasta\_13\_mtDNA\_trimmed\_named.fas **Description:** Aligned and trimmed FASTA file of 13 mtDNA genes used to build a neighbor-joining tree of 5 individual kingbirds #### File: Consensus\_H1517\_Hybrid\_female\_mtDNA.unpadded.fasta **Description:** Output file of MITObim used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: Consensus\_H3020\_Fledgling\_A\_mtDNA.unpadded.fasta **Description:** Output file of MITObim used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: Consensus\_W1524\_Pure\_female\_mtDNA.unpadded.fasta **Description:** Output file of MITObim used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: Consensus\_W3017\_Fledgling\_B\_mtDNA.unpadded.fasta **Description:** Output file of MITObim used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: consensus\_SOK009\_Scissor\_tailed\_outgroup\_mtDNA.unpadded.fasta **Description:** Output file of MITObim used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: H1517\_Hybrid\_Female\_MITOS.gff **Description:** Output file of MITOS used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: H3020\_Fledgling\_A\_MITOS.gff **Description:** Output file of MITOS used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: SOK009\_Scissor\_tailed\_outgroup\_MITOS.gff **Description:** Output file of MITOS used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: W1524\_pure\_female\_MITOS.gff **Description:** Output file of MITOS used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: W3017\_Fledgling\_B\_MITOS.gff **Description:** Output file of MITOS used to extract 13 mtDNA protein-coding genes for 5 kingbirds #### File: neighbor\_joining\_phylogney\_of\_5\_individuals\_using\_13\_mtDNA\_genes.tre **Description:** Neighbor-joining tree built in R with package ape using K80 nuclear subsitution model #### File: neighbor\_joining\_phylogney\_of\_5\_individuals\_using\_13\_mtDNA\_genes.png **Description:** Neighbor-joining tree of 5 individual kingbirds #### File: 8.joint.dp.maxdp.mp2.qual.pass.missdata.maf.m2.relatedness2 **Description:** Generated output matrix from Relatedness2 to assess relatedness of 8 individuals #### File: 8.joint.dp.maxdp.mp2.qual.pass.missdata.maf.m2.vcf **Description:** Filtered VCF file used as input in Relatedness2 analysis generated to assess relatedness of 8 individuals #### File: HybirdFemale\_PureFemale\_coincubating.JPG **Description:** Photo of both adults co-incubating shared nest #### File: FledglingA\_FledglingB\_comparison.jpg **Description:** Photo comparison of Fledgling A and Fledgling B in the hand #### File: Fledglings\_in\_shared\_nest.JPG **Description:** Photo of 4 nestlings in shared nest ## Code/Software #### File: Code\_for\_genomic\_data\_and\_relatedness\_analyses.txt **Description:** Bash code used to quality check and trim raw reads, generate genome assemblies, call and filter SNPs, perform Relatedness2, and extract mtDNA genes #### File: Code\_for\_neighbor\_joining\_tree\_in\_R.txt **Description:** Code used to construct neighbor-joining distance tree in R