cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/0t4ms-x7540
title: Haloferax gibbonsii

description: Haloferax gibbonsii
description: <strong>Tilt Series Date:</strong> 2014-06-05</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Haloferax gibbonsii</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 1600.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
  aba2014-06-05-5_slicer3992.jpg: Haloferax gibbonsii cell attachemnt structure aba2014-06-05-5_slicer3993.jpg: Haloferax gibbonsii cell attachemnt structure aba2014-06-05-5_slicer3994.jpg: Haloferax gibbonsii cell attachemnt structure aba2014-06-05-5_slicer3995.jpg: Haloferax gibbonsii cell attachemnt structure
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-06-05-5/3dimage_43133/</p>Haloferax160eA0005_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-06-05-5/3dimage_43133/Haloferax160eA0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Haloferax160eA0005.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-06-05-5/rawdata/Haloferax160eA0005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bwhyj-71w13
title: Agrobacterium tumefaciens A139

description: Bald strain with GFP tagged VirB8
description: <strong>Tilt Series Date:</strong> 2014-01-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown on plate, scraped off and grown o/n in a drop</p>
<strong>Sample Preparation:</strong> Vitrobot manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-6/rawdata/</p>A139_drop0006.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-6/rawdata/A139_drop0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        A139_drop0006_part121_10.rec,  Reconstruction (Pixel Size 0.0 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-6/3dimage_49306/A139_drop0006_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hpkkh-w6s95
title: Pseudoalteromonas Iuteoviolacea MP129

description: P Iuteo DvipABhcpI Dbact2 whole cells from liquid culture
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab, taken at Janelia Farms</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid culture</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
  mpa2013-07-12-17_slicer8615.jpg: Pseudoalteromonas iuteoviolacea .  Ribosome excluding zone.  Nucleoid?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-17/rawdata/</p>2013_07_12_JF_MP129_b_0012.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-17/rawdata/2013_07_12_JF_MP129_b_0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_07_12_JF_MP129_b_0012_2k_secs_full.rec,  Reconstruction (Pixel Size 1.68 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-17/3dimage_28948/2013_07_12_JF_MP129_b_0012_2k_secs_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ctv4r-9pe59
title: Bsubtilis_wildtype_168_sacculi

description: Bsubtilis_168_sacculi_withTA
description: <strong>Tilt Series Date:</strong> 2012-09-01</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> subsp. subtilis str. 168</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-01-9/rawdata/</p>mj_thin0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-01-9/rawdata/mj_thin0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        mj_thin4_full.rec,  Reconstruction (Pixel Size 2.0 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-01-9/3dimage_25651/mj_thin4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sr96e-2qt34
title: HUVECs infx pNLGFP

description: pNLGFP obtained from Irvin Chen (UCLA)
description: <strong>Tilt Series Date:</strong> 2013-10-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 36h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-22-6/rawdata/</p>C169-2_HUVECs_pNL36h_atlas3_0005.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-22-6/rawdata/C169-2_HUVECs_pNL36h_atlas3_0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        C169-2_HUVECs_pNL36h_atlas3_0005_part121_1.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-22-6/3dimage_33646/C169-2_HUVECs_pNL36h_atlas3_0005_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r1h1y-qvh06
title: Pseudoalteromonas Iuteoviolacea MP123

description: P Iuteo DvipABhcpI Dbact2
description: <strong>Tilt Series Date:</strong> 2013-06-23</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> on plate</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-23-4/rawdata/</p>2013_06_23_mp123_0005.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-23-4/rawdata/2013_06_23_mp123_0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_06_23_mp123_0005_2k_part121_69.rec,  Reconstruction (Pixel Size 1.97 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-23-4/3dimage_28872/2013_06_23_mp123_0005_2k_part121_69.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mpypk-bbh90
title: Crosslinked HIV-1 cores TRIM5a

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5alpha.
description: <strong>Tilt Series Date:</strong> 2013-05-24</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 99.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor and SIRT reconstruction</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-24-27/3dimage_42911/</p>montage0004_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-24-27/3dimage_42911/montage0004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        montage0004.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-24-27/rawdata/montage0004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.pfxjke
title: Occurrence Download

description: A dataset containing 930 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "PublishingCountry is Colombia",
 {
 "and" : [
 "Country is Colombia",
 {
 "not" : {
 "Issue" : [
 "is Country coordinate mismatch"
 ]
 }
 }
 ]
 }
 ]
 },
 "Geometry MULTIPOLYGON(((-79.51467 4.47162,-78.11895 4.47162,-78.11895 6.15271,-79.12835 6.15271,-79.23333 6,-79.51467 5.55578,-79.51467 4.47162)))"
 ]
}

 The dataset includes 930 records from 46 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047662-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/a520y-f6e69
title: Agrobacterium tumefaciens

description: C58 wild type
description: <strong>Tilt Series Date:</strong> 2013-07-28</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Polara</p>
<strong>Collaborators and Roles:</strong> Pat Zabryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> C58 wild type</p>
<strong>Sample Preparation:</strong> 200uM AS, grown on agar + liquid for 2 days</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-28-17/rawdata/</p>c58_au20017.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-28-17/rawdata/c58_au20017.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_au20017_part121_26.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-28-17/3dimage_29431/c58_au20017_part121_26.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qwy5m-69h84
title: TRIM5-21R delSpry

description: TRIM5-21R delSpry 2D lattice prepared by Barbie Ganser-Pornillos and  Viswanathan Chandrasekaran
description: <strong>Tilt Series Date:</strong> 2012-11-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Viswanathan Chandrasekarangrids purified the protein, Barbie Ganser-Pornillos assembled protein into 2D lattice. The sample was was plunge frozen and imaged at Caltech by Cora Woodward</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-22-1/rawdata/</p>12nov22_TRIM5_21R_delSpry_grid3_AT5BT10001.mrc,  Tilt Series (Pixel Size 0.45 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-22-1/rawdata/12nov22_TRIM5_21R_delSpry_grid3_AT5BT10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        12nov22_TRIM5_21R_delSpry_grid3_AT5BT10001_full.rec,  Reconstruction (Pixel Size 1.8 nm), 353.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-22-1/3dimage_23814/12nov22_TRIM5_21R_delSpry_grid3_AT5BT10001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9kqyq-rxd15
title: HUVECs infx pNLGFP

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 19000.
description: <strong>Tilt Series Date:</strong> 2013-07-05</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Sample Preparation:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-10/rawdata/</p>13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0019.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-10/rawdata/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0019.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0019_full.rec,  Reconstruction (Pixel Size 2.24 nm), 602.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-10/3dimage_28046/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ts0y1-jr147
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-17/3dimage_68606/</p>12apr28_C126_1_pNLDN_COS7_AT2BT3_0027_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-17/3dimage_68606/12apr28_C126_1_pNLDN_COS7_AT2BT3_0027_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_1_pNLDN_COS7_AT2BT3_0027.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-17/rawdata/12apr28_C126_1_pNLDN_COS7_AT2BT3_0027.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ck9p0-wka29
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-3/3dimage_68574/</p>12apr28_C126_2_pNLDN_COS7_AT4_man1_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-3/3dimage_68574/12apr28_C126_2_pNLDN_COS7_AT4_man1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT4_man1.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-3/rawdata/12apr28_C126_2_pNLDN_COS7_AT4_man1.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5zqpv-dfp20
title: Agro VirB8-GFP

description: Raw data files of Agro VirB8-GFP
description: <strong>Tilt Series Date:</strong> 2013-10-03</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown in liquid at 19C and induced with 200uM AS for 24 hours</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-23/rawdata/</p>virb8_24h_2750024.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-23/rawdata/virb8_24h_2750024.mrc"
        > <i class="download icon"></i> Download </a></p>

        virb8_24h_2750024_full.rec,  Reconstruction (Pixel Size 1.56 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-03-23/3dimage_31859/virb8_24h_2750024_full.rec"
        > <i class="download icon"></i> Download </a></p>