cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11997885
title: The combined risk effect among BIN1, CLU, and APOE genes in Alzheimer’s disease

description: Abstract Genome-wide associations studies (GWAS) are detecting new variants associated with late-onset of Alzheimer’s disease (LOAD), a multifactorial neurodegenerative disorder. The variants rs744373 BIN1, rs11136000 CLU and rs3764650 ABCA7 uncovered by GWAS led to different AD pathways, such as metabolism, trafficking and endocytosis of lipids and inflammation. However, most of the association studies did not replicate these variants with significance. This could be due to a small power effect evident when these variants are tested independently with LOAD. Therefore, we aimed to investigate whether the combination of different variants would additively modify the risk of association with LOAD that is observed in GWAS. We performed an association study testing pairwise variants in metabolism, trafficking and endocytosis of lipid (rs429358 and rs7412 APOE, rs744373 BIN1, rs3764650 ABCA7 and rs11136000 CLU) pathways with LOAD in samples from southeastern Brazil. Our data suggest a risk effect for LOAD between APOE with CLU and APOE with BIN1 genes.
DOI: 10.6084/m9.figshare.11997891.v1
title: Effects of storage on vis-NIR-SWIR reflectance spectra of Mombasa grass leaf samples

description: ABSTRACT: Vis-NIR-SWIR reflectance spectra of leaf samples, collected in the laboratory, allow the calibration of predictive models to quantify their physicochemical attributes in a practical manner and without producing chemical residues. This technique should enable the development of management strategies for intensification of pasture use. However, spectral analysis performed in the laboratory may be affected by the deterioration of plant material during transport from the field to the lab, so storage methods are necessary. This research aimed to evaluate the effects of different storage methods on the spectral response of Mombasa grass leaves. Three methods were evaluated: (i) artificially refrigerated environment, (ii) humid environment, and (iii) without microenvironment control. These methods were tested in five different storage times: 2 hours, 4 hours, 8 hours, 24 hours and 48 hours. The spectral behavior of the leaves still inserted in the plant was used as a quality reference. Results showed notable changes at the earliest storage time for the treatment without microenvironment control. Both methods with microenvironment control stabilized the occurrence of spectral changes over 48 hours of the samples storage, thus both were suggested for this species.
DOI: 10.6084/m9.figshare.11997879
title: Involvement of lipid microdomains in human endothelial cells infected by Streptococcus agalactiae type III belonging to the hypervirulent ST-17

description: BACKGROUND Streptococcus agalactiae capsular type III strains are a leading cause of invasive neonatal infections. Many pathogens have developed mechanisms to escape from host defense response using the host membrane microdomain machinery. Lipid rafts play an important role in a variety of cellular functions and the benefit provided by interaction with lipid rafts can vary from one pathogen to another. OBJECTIVES This study aims to evaluate the involvement of membrane microdomains during infection of human endothelial cell by S. agalactiae. METHODS The effects of cholesterol depletion and PI3K/AKT signaling pathway activation during S. agalactiae-human umbilical vein endothelial cells (HUVEC) interaction were analysed by pre-treatment with methyl-β-cyclodextrin (MβCD) or LY294002 inhibitors, immunofluorescence and immunoblot analysis. The involvement of lipid rafts was analysed by colocalisation of bacteria with flotillin-1 and caveolin-1 using fluorescence confocal microscopy. FINDINGS In this work, we demonstrated the importance of the integrity of lipid rafts microdomains and activation of PI3K/Akt pathway during invasion of S. agalactiae strain to HUVEC cells. Our results suggest the involvement of flotillin-1 and caveolin-1 during the invasion of S. agalactiae strain in HUVEC cells. CONCLUSIONS The collection of our results suggests that lipid microdomain affects the interaction of S. agalactiae type III belonging to the hypervirulent ST-17 with HUVEC cells through PI3K/Akt signaling pathway.
DOI: 10.6084/m9.figshare.11997882
title: Modeling and simulation of an intelligent system for dosage control of post-chlorination in water treatment plants

description: ABSTRACT This paper presents an approach using a neural network model to control chlorine dosage in treated water, so as to obtain better results in the stability of the free residual chlorine, when compared to the control currently used, based on the Proportional-Integral-Derivative (PID) model and the consequent reduction in chlorine consumption in the water treatment process. Among the different disinfectants, chlorine is widely used in large water treatment plants in the world. In Brazil, especially in large cities, this chemical product is also used as a disinfectant agent. In this context, a neural model was implemented and simulated in MatLab software, taking into account the operational characteristics of a treatment plant, located in the São Paulo Metropolitan Region.
DOI: 10.6084/m9.figshare.11997885.v1
title: The combined risk effect among BIN1, CLU, and APOE genes in Alzheimer’s disease

description: Abstract Genome-wide associations studies (GWAS) are detecting new variants associated with late-onset of Alzheimer’s disease (LOAD), a multifactorial neurodegenerative disorder. The variants rs744373 BIN1, rs11136000 CLU and rs3764650 ABCA7 uncovered by GWAS led to different AD pathways, such as metabolism, trafficking and endocytosis of lipids and inflammation. However, most of the association studies did not replicate these variants with significance. This could be due to a small power effect evident when these variants are tested independently with LOAD. Therefore, we aimed to investigate whether the combination of different variants would additively modify the risk of association with LOAD that is observed in GWAS. We performed an association study testing pairwise variants in metabolism, trafficking and endocytosis of lipid (rs429358 and rs7412 APOE, rs744373 BIN1, rs3764650 ABCA7 and rs11136000 CLU) pathways with LOAD in samples from southeastern Brazil. Our data suggest a risk effect for LOAD between APOE with CLU and APOE with BIN1 genes.
DOI: 10.6084/m9.figshare.11997873
title: Water productivity with localized irrigation using groundwater and reuse water in the cultivation of plant species

description: ABSTRACT This study aimed to analyze the water saving potential of replacing the traditional, furrow-irrigated banana crop in the Curu-Pentecoste Irrigated Perimeter with localized systems by inserting new cropping plans and sources of groundwater and reuse water. An analysis of the support capacity in shallow tube wells for irrigation was also performed, considering the scenario of full recharge and water scarcity. The economic analysis was performed using the profitability indicators of the investment analysis: benefit/cost ratio, net present value, internal rate of return and payback period. Water productivity data reveal that cropping plans associated with alternative sources of water from shallow tube wells and reuse of the excess surface irrigation water in localized systems are economically more attractive than traditional, furrow-irrigated banana crop. The support capacity of shallow tube wells reveals that the exploitation of this underground water resource in the region is a viable alternative for irrigation of small areas. With the exception of banana, the other scenarios analyzed showed positive cash flows from the second year.
DOI: 10.6084/m9.figshare.11997876
title: Phosphorylated protein concentrate pumpkin seed (Cucurbita moschata): optimization by response surface methodology and nutritional characterization

description: ABSTRACT: Response surface methodology was used to determine optimum conditions for extraction of protein from pumpkin seed meal. A central composite rotational design (CCRD) including independent variables such, pH and sodium trimetaphosphate (STMP) content was used. Maximum yield was obtained at a central point of CCRD, with STMP content and pH of 4% and 4.5, respectively. These conditions resulted in protein yield of 50.04 g of soluble protein from extract/100 g pumpkin seed. The optimization of pH and STMP content allowed obtaining a product with a high protein concentration (62.56 g 100 g-1), digestibility (62.03 g 100 g-1) and concentration of essential amino acids (27.26 g 100 g-1). Regarding the polyphenols concentration, phosphorylated protein concentrate from pumpkin seed and the pumpkin seed meal presented 13.11 g 100 g-1 and 23.19 g 100 g-1, respectively. The adequacy of the model was confirmed by extracting the protein under optimum values. These results help in designing the process of optimal protein extraction from pumpkin seeds.
DOI: 10.6084/m9.figshare.11997879.v1
title: Involvement of lipid microdomains in human endothelial cells infected by Streptococcus agalactiae type III belonging to the hypervirulent ST-17

description: BACKGROUND Streptococcus agalactiae capsular type III strains are a leading cause of invasive neonatal infections. Many pathogens have developed mechanisms to escape from host defense response using the host membrane microdomain machinery. Lipid rafts play an important role in a variety of cellular functions and the benefit provided by interaction with lipid rafts can vary from one pathogen to another. OBJECTIVES This study aims to evaluate the involvement of membrane microdomains during infection of human endothelial cell by S. agalactiae. METHODS The effects of cholesterol depletion and PI3K/AKT signaling pathway activation during S. agalactiae-human umbilical vein endothelial cells (HUVEC) interaction were analysed by pre-treatment with methyl-β-cyclodextrin (MβCD) or LY294002 inhibitors, immunofluorescence and immunoblot analysis. The involvement of lipid rafts was analysed by colocalisation of bacteria with flotillin-1 and caveolin-1 using fluorescence confocal microscopy. FINDINGS In this work, we demonstrated the importance of the integrity of lipid rafts microdomains and activation of PI3K/Akt pathway during invasion of S. agalactiae strain to HUVEC cells. Our results suggest the involvement of flotillin-1 and caveolin-1 during the invasion of S. agalactiae strain in HUVEC cells. CONCLUSIONS The collection of our results suggests that lipid microdomain affects the interaction of S. agalactiae type III belonging to the hypervirulent ST-17 with HUVEC cells through PI3K/Akt signaling pathway.
DOI: 10.6084/m9.figshare.11997873.v1
title: Water productivity with localized irrigation using groundwater and reuse water in the cultivation of plant species

description: ABSTRACT This study aimed to analyze the water saving potential of replacing the traditional, furrow-irrigated banana crop in the Curu-Pentecoste Irrigated Perimeter with localized systems by inserting new cropping plans and sources of groundwater and reuse water. An analysis of the support capacity in shallow tube wells for irrigation was also performed, considering the scenario of full recharge and water scarcity. The economic analysis was performed using the profitability indicators of the investment analysis: benefit/cost ratio, net present value, internal rate of return and payback period. Water productivity data reveal that cropping plans associated with alternative sources of water from shallow tube wells and reuse of the excess surface irrigation water in localized systems are economically more attractive than traditional, furrow-irrigated banana crop. The support capacity of shallow tube wells reveals that the exploitation of this underground water resource in the region is a viable alternative for irrigation of small areas. With the exception of banana, the other scenarios analyzed showed positive cash flows from the second year.
DOI: 10.6084/m9.figshare.11997876.v1
title: Phosphorylated protein concentrate pumpkin seed (Cucurbita moschata): optimization by response surface methodology and nutritional characterization

description: ABSTRACT: Response surface methodology was used to determine optimum conditions for extraction of protein from pumpkin seed meal. A central composite rotational design (CCRD) including independent variables such, pH and sodium trimetaphosphate (STMP) content was used. Maximum yield was obtained at a central point of CCRD, with STMP content and pH of 4% and 4.5, respectively. These conditions resulted in protein yield of 50.04 g of soluble protein from extract/100 g pumpkin seed. The optimization of pH and STMP content allowed obtaining a product with a high protein concentration (62.56 g 100 g-1), digestibility (62.03 g 100 g-1) and concentration of essential amino acids (27.26 g 100 g-1). Regarding the polyphenols concentration, phosphorylated protein concentrate from pumpkin seed and the pumpkin seed meal presented 13.11 g 100 g-1 and 23.19 g 100 g-1, respectively. The adequacy of the model was confirmed by extracting the protein under optimum values. These results help in designing the process of optimal protein extraction from pumpkin seeds.
DOI: 10.6084/m9.figshare.11997864
title: Austenitizing Temperature and Cooling Rate Effects on the Martensitic Transformation in a Microalloyed-Steel

description: Abstract The effects of the austenitizing temperature and the cooling rate upon the kinetic of athermal martensitic transformation in a microalloyed steel were evaluated. Considering the studied steel, the knowledge about these effects on the martensitic transformation has a great relevance for naval manufacturers and steel researchers. In this study, computational simulation was performed aiming to evaluate the phase’s stability. Specimens were submitted to quenching simulations in a dilatometer, considering four different austenitizing temperatures and four cooling rates. The results shown that the austenite chemical composition was not significantly affected by the austenitizing temperatures. Both the austenitic grain size and the cooling rate affected the martensitic transformation kinetics. The larger the austenitic grain size, the higher the Ms. The austenitic grain growth promoted a decrease in the required chemical energy to compensate the free energy increase associated with the lattice strain and the creation of new interfaces, leading to a lower austenite undercooling. An extrinsic effect of the cooling rate on the Ms was observed. For lower cooling rates, the carbide precipitation modified que austenite chemical composition, changing its stability and increasing Ms. A predictability equation, correlating the MS with the austenite grain size and the steel cooling rate, was proposed.
DOI: 10.6084/m9.figshare.11997867
title: Isotherms and thermodynamic properties of water adsorption in ‘Cumari-do-Pará’ pepper seeds

description: ABSTRACT Studies related to water sorption in seeds are essential for the design and optimization of storage systems. The objective of this research was to determine and model the adsorption isotherms and calculate the latent heat of water vaporization, differential enthalpy and entropy, the isokinetic theory and Gibbs free energy for ‘Cumari-do-Pará’ pepper seeds. The equilibrium moisture contents were obtained by the static gravimetric method at temperatures of 30, 35 and 40 °C and water activities between 0.290 and 0.900 (decimal). The Chen-Clayton model is the one that best represents the water adsorption isotherms in ‘Cumari-do-Pará’ pepper seeds under the studied conditions, with 9.94% mean relative error, 0.40 mean estimated error and random distribution of residuals. The latent heat of vaporization ranged from 2,555.669 to 3,162.180 kJ kg-1. The enthalpy, entropy and Gibbs free energy increase with the reduction in the equilibrium moisture content of the seeds. The isokinetic theory is valid for the adsorption process.
DOI: 10.6084/m9.figshare.11997864.v1
title: Austenitizing Temperature and Cooling Rate Effects on the Martensitic Transformation in a Microalloyed-Steel

description: Abstract The effects of the austenitizing temperature and the cooling rate upon the kinetic of athermal martensitic transformation in a microalloyed steel were evaluated. Considering the studied steel, the knowledge about these effects on the martensitic transformation has a great relevance for naval manufacturers and steel researchers. In this study, computational simulation was performed aiming to evaluate the phase’s stability. Specimens were submitted to quenching simulations in a dilatometer, considering four different austenitizing temperatures and four cooling rates. The results shown that the austenite chemical composition was not significantly affected by the austenitizing temperatures. Both the austenitic grain size and the cooling rate affected the martensitic transformation kinetics. The larger the austenitic grain size, the higher the Ms. The austenitic grain growth promoted a decrease in the required chemical energy to compensate the free energy increase associated with the lattice strain and the creation of new interfaces, leading to a lower austenite undercooling. An extrinsic effect of the cooling rate on the Ms was observed. For lower cooling rates, the carbide precipitation modified que austenite chemical composition, changing its stability and increasing Ms. A predictability equation, correlating the MS with the austenite grain size and the steel cooling rate, was proposed.
DOI: 10.6084/m9.figshare.11997858
title: Yield and must composition of grapevines subjected to phosphate fertilization in Southern Brazil

description: Abstract: The objective of this work was to evaluate the yield and must composition of 'Cabernet Sauvignon' and 'Chardonnay' grapevines subjected to phosphorus applications to a soil from a high-altitude region of Southern Brazil, during three crop seasons. Experiments 1 and 2 were carried out in 'Cabernet Sauvignon' and 'Chardonnay' commercial vineyards, respectively, in the municipality of Água Doce, located in the Midwestern region of the state of Santa Catarina. The soil from the two vineyards was classified as a Typic Humicryept. The used source of P was triple superphosphate (45% P2O5). From 2011 to 2013, at flowering, a total of 0, 10, 20, 40, and 80 kg ha-1 P2O5 were applied in the crown projection area, to the soil surface, without incorporation, in both vineyards. Soil and leaf P concentration, yield parameters, and must quality were evaluated. The increase in P availability decreased total titratable acidity and tartaric acid in the must of the 'Cabernet Sauvignon' grapevine in the 2013/2014 crop season and increased pH and total soluble solids in the must of the 'Chardonnay' grapevine in the 2011/2012 crop season. Phosphorus application to the soil increases the levels of available phosphorus, but does not result in higher yields for 'Cabernet Sauvignon' and 'Chardonnay' grapevines.
DOI: 10.6084/m9.figshare.11997861
title: Urban drainage modeling and evaluation of bacteriological loads in the Vertente Marítima of Fortaleza, Ceará

description: ABSTRACT Urban drainage systems can represent sources of pollutants input to the receiving bodies for which they are intended. Therefore, the present work aims to propose a tool for modeling the urban drainage system of the Vertente Marítima basin of Fortaleza, Ceará, aiming at the evaluation of bacteriological loads. For this purpose, the Stormwater Management Model (SWMM) was selected for the hydrological and hydraulic simulations; a methodology for the calculation of bacteriological loads was proposed by estimating the diffuse load [DL] (MPN, day-1.ha-1) and its correlation with a series of hydro-environmental variables and scenarios for different pluviometry precipitation conditions were analyzed, as well as sewage collection and inadequately disposed solid waste (IDSW). The results show that SWMM gives representative results and that the rainy season is responsible for the greater contribution of the bacteriological load to the seafront. Regarding the predictive model, a second-order potential function was adjusted by correlating DL with the average monthly precipitation (mm.day-1) for the three sub-basins analyzed. Subsequently, the coefficients of the equations found were correlated to IDSW (kg.day-1), obtaining a logarithmic adjustment (R2 = 0.99), with which it is possible to obtain a DL estimation equation for any sub-basin of Vertente Marítima. An analysis of the scenarios showed a significant increase of the load in extreme precipitations (twice) and a considerable decrease with the increase of the fraction of the population connected to the sewage network and the decrease of IDSW production (0.28 and 0.38 times the initial value in the Jacarecanga and Maceió Stream, respectively).
DOI: 10.6084/m9.figshare.11997861.v1
title: Urban drainage modeling and evaluation of bacteriological loads in the Vertente Marítima of Fortaleza, Ceará

description: ABSTRACT Urban drainage systems can represent sources of pollutants input to the receiving bodies for which they are intended. Therefore, the present work aims to propose a tool for modeling the urban drainage system of the Vertente Marítima basin of Fortaleza, Ceará, aiming at the evaluation of bacteriological loads. For this purpose, the Stormwater Management Model (SWMM) was selected for the hydrological and hydraulic simulations; a methodology for the calculation of bacteriological loads was proposed by estimating the diffuse load [DL] (MPN, day-1.ha-1) and its correlation with a series of hydro-environmental variables and scenarios for different pluviometry precipitation conditions were analyzed, as well as sewage collection and inadequately disposed solid waste (IDSW). The results show that SWMM gives representative results and that the rainy season is responsible for the greater contribution of the bacteriological load to the seafront. Regarding the predictive model, a second-order potential function was adjusted by correlating DL with the average monthly precipitation (mm.day-1) for the three sub-basins analyzed. Subsequently, the coefficients of the equations found were correlated to IDSW (kg.day-1), obtaining a logarithmic adjustment (R2 = 0.99), with which it is possible to obtain a DL estimation equation for any sub-basin of Vertente Marítima. An analysis of the scenarios showed a significant increase of the load in extreme precipitations (twice) and a considerable decrease with the increase of the fraction of the population connected to the sewage network and the decrease of IDSW production (0.28 and 0.38 times the initial value in the Jacarecanga and Maceió Stream, respectively).
DOI: 10.6084/m9.figshare.11997867.v1
title: Isotherms and thermodynamic properties of water adsorption in ‘Cumari-do-Pará’ pepper seeds

description: ABSTRACT Studies related to water sorption in seeds are essential for the design and optimization of storage systems. The objective of this research was to determine and model the adsorption isotherms and calculate the latent heat of water vaporization, differential enthalpy and entropy, the isokinetic theory and Gibbs free energy for ‘Cumari-do-Pará’ pepper seeds. The equilibrium moisture contents were obtained by the static gravimetric method at temperatures of 30, 35 and 40 °C and water activities between 0.290 and 0.900 (decimal). The Chen-Clayton model is the one that best represents the water adsorption isotherms in ‘Cumari-do-Pará’ pepper seeds under the studied conditions, with 9.94% mean relative error, 0.40 mean estimated error and random distribution of residuals. The latent heat of vaporization ranged from 2,555.669 to 3,162.180 kJ kg-1. The enthalpy, entropy and Gibbs free energy increase with the reduction in the equilibrium moisture content of the seeds. The isokinetic theory is valid for the adsorption process.