cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/srb7pskmy3
title: BasC smFRET experiments

description: Raw data to make the figures of the article: 
The conserved lysine residue in transmembrane helix 5 is key for the cytoplasmic gating of the L-amino acid transporters
Authors: Joana Fort*, Adrià Nicolàs-Aragó, Luca Maggi, Maria Martinez Molledo, Despoina Kapiki, Paula Gonzalez-Novoa, Patricia Gómez-Gejo, Niels Zijlstra, Susanna Bodoy, Els Pardon, Jan Steyaert, Oscar Llorca, Modesto Orozco, Thorben Cordes,*, Manuel Palacín* 
* corresponding Authors
To monitor the movement of the inner gate of BasC in solution at room temperature, we developed a smFRET assay that tracks its conformational changes. To this end, two distinct double-cysteine variants of BasC were designed. Ile7 in TM1a was combined with two other residues in the protein: Thr120 in TM4, forming the TM1a-TM4 pair, and Cys427, the only natural cysteine in BasC, in TM12, forming the TM1a-TM12 pair (Fig. 1A).
ALEX-smFRET microscopy
All samples were examined by focusing the excitation/observation volume of a confocal microscope into a ~100 μl sample drop containing ~50 pM of BasC in DDM buffer on a glass coverslip. Before data recording, the coverslips were coated for &gt;60 s with 1 mg/mL bovine serum albumin to prevent fluorophore and/or protein adsorption. Each experimental condition (apo, ligand etc.) was then studied for between 30 and 120 min, depending on the fraction of donor- and acceptor-containing BasC molecules in relation to donor- and acceptor-only ones. We also performed DNA controls with the same fluorophore pairs as for the proteins in DDM buffer to verify proper microscope alignment and to exclude buffer interferences.
For alternating laser excitation, we used two laser sources for sample excitation with electronic modulation: a 532 nm diode laser at 60 μW (OBIS 532-100-LS, Coherent, USA) and a diode laser at 640 nm (OBIS 640-100-LX, Coherent, USA) at 25 or 30 μW for Alexa 546-Alexa 647 and sCy3-sCy5 dye-pairs, respectively. The laser light was guided into an epi-illuminated confocal microscope body (Olympus IX71, Hamburg, Germany) by a dual-edge beamsplitter (ZT532/640rpc, Chroma/AHF, Germany) and focused to a diffraction-limited excitation spot by a water immersion objective (UPlanSApo 60x/1.2w, Olympus Hamburg, Germany). The emitted fluorescence was collected through the same objective, spatially filtered using a pinhole with a diameter of 50 μm and spectrally split into donor and acceptor channel by a single-edge dichroic mirror H643 LPXR (AHF). Fluorescence emission was filtered (donor: BrightLine HC 582/75 (Semrock/AHF), acceptor: Longpass 647 LP Edge Basic (Semroch/AHF)) and focused onto avalanche photodiodes (SPCM-AQRH-64, Excelitas). The detector outputs were recorded by a NI-Card (PCI-6602, National Instruments, USA).
data
DOI: 10.3535/caa-1m7-0d2
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/k44-kak-zp3
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/11c-me1-efp
title: Conchicolites sp.

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/ffn-bzp-23h
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/yxw-xbl-8kz
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/zwa-2lm-eld
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/8by-gjq-lsw
title: Animalia

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/lc6-zfm-gy5
title: Graptolithina Bronn, 1849

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/982-qse-4f8
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/tkc-2nt-7ah
title: Tuberoconchus wilsoni

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/1pr-meq-twb
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/11a-v8r-p37
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/hwz-4tp-syx
title: †Protochonetes Muir-Wood, 1962

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/6x0-kpd-yez
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/n6l-nb5-m3x
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/9q7-ts3-lev
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/xyh-1qj-qv1
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/frm-qng-23c
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/h3h-mbp-098
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/3kr-j6d-02b
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/x58-gmx-w3x
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/saj-5ce-fg0
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/scd-g5w-lhh
title: Geyserite

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/3ba-25n-bzw
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/8mq-7sc-7el
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/jhz-zns-xm9
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/q08-xfz-9mf
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/hjb-x0x-445
title: Favositidae

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/s3r-tkg-cz0
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/84x-7kx-36a
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/1t5-yp6-8gp
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/ey0-eeh-6g7
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/qd6-kjm-trv
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/t90-9zp-k84
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/b6k-xy6-l0s
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/mx5-cd0-3ns
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/s0j-xjw-5ba
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/6s1-k1h-svw
title: Favositidae

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/94r-e5h-qhc
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/qh5-010-zd0
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/zdb-lkz-888
title: †Isorthis Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/7ce-96x-0fb
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/9s6-6jg-3qs
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/65z-lhy-qqs
title: Favositidae

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/kqa-l0v-wkv
title: Favositidae

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/pn6-t1h-whj
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/wcq-p35-wy0
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/7b1-7nt-pgz
title: Favositidae

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/90x-jy4-m29
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/tpb-gb1-lex
title: †Skenidioides Schuchert & Cooper, 1931

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/exz-vxl-4np
title: †Leangella Öpik, 1933

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/n82-n2j-df1
title: Favositidae

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.6084/m9.figshare.19130495.v1
title: Genetic slippage after sex maintains diversity for parasite resistance in a natural host population

description: Data associated to the manuscript:<br> Ameline, C., Vögtli, F., Andras, J.P., Dexter, E., Engelstädter, J., Ebert, D., 2022. Genetic slippage after sex maintains diversity for parasite resistance in a natural host population. Science Advances 8(46):eabn0051, DOI: 10.1126/sciadv.abn0051 <br> <br> List of files: <br> <br> ################################################################## <br> Script #1: sampling and hatching plots <br> <br> - dynamics_ScAdv_sampling-hatching.R: R script <br> The R script uses the following files: <br> <br> 1. Field monitoring of resistance, prevalence and ephippia laying <br> - chh.txt: resistotype, prevalence and ephippia laying data for 2010 to 2018 - chh4l.txt: same as chh, but with 4-latter resistotypes for the hatching plot - chhcolorcode.txt: color code for resistotypes - chhn.txt: sample sizes to annotate Fig. 1 - resstat.txt: resistotype values of each P. ramosa isolate for each year for mean and se calculation - chh2021.txt: sampling data for 2020 and 2021 for Fig. S8 <br> 2. Slippage plot <br> - slippage.txt: data for slippage plot, Fig. 2 <br> 3. Hatching plots <br> - prophatch_all_genos.xlsx: for calculations of expected resistotypes proportions from hatching of resting eggs - prophatch_bbDDfreqCE.xlsx: same as above - legend_chhhatching_expected.png: legend of hatching figures - scatterhatch.txt: for Fig. 4 - legend_scatterhatch.png: legend Fig. 4 <br> 4. Hathcing experiment Fig. S3 <br> figure - eggbanknum.csv - eggbankrpe.txt - eggbank14.txt <br> stats - spring14_hatch.txt - spring14eggbank.txt - spring15eggbank.txt - spring16eggbank.txt - spring18eggbank.txt <br> 5. Resting stages laying, counting and hatching success Fig. S2 <br> - hatchdates.txt - hatching14151718.txt <br> <br> ################################################################## <br> Script #2: modelling the genetic model of resistance using peas and matrices <br> <br> - dynamics_ScAdv_modelling.R: R script <br> The R script uses the following files: <br> <br> - chh4l.txt: to get the resistotype proportions at each timepoint <br> user input of allele frequency within the different resistotypes: - freq_all_genos.csv - freq_all.csv - freq_all_bbDDfreqCE.xlsx - freq_all_onegeno.xlsx <br> output files from the script - prophatch_all_genos.xlsx - prophatch_all_onegeno.xlsx - prophatch_bbDDfreqCE.xlsx <br> <br> ################################################################## <br> Script #3: environmental variables - Fig. S1 <br> <br> - dynamics_ScAdv_env.R: R script <br> The R script uses the following files: <br> <br> - chh.txt: resistotype, prevalence and ephippia laying data for 2010 to 2018 - chh_temp_201119.csv: data from temperature logger in the pond - chhvar.txt: sampling variables: water level, daphnia species, sexes, density. <br> <br> ##################################################################
DOI: 10.3535/0k7-qs2-lt9
title: Glauconitic sandstone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/8j0-jks-x5f
title: glauconitic limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/xkr-gpz-w59
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/zbf-zg9-x4g
title: †Skenidioides Schuchert & Cooper, 1931

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/86l-txt-v91
title: glauconitic limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/1xx-erf-dn4
title: sedimentary rocks

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/kh5-fz6-11f
title: glauconitic limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/6vm-p21-q6v
title: Bryozoa

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/mh4-sv8-ys9
title: †Eoplectodonta Kozlowski, 1929

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/2ac-mfw-r3l
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/qvy-hj4-8tp
title: Porifera Grant, 1836

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/b3q-r8y-6dq
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/s19-mvj-n1s
title: Haplacanthus marginalis

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/0gj-lsw-svn
title: Devononchus concinnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/gyz-t4q-840
title: Microbioerosional trace fossils

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/qq8-v2d-a1g
title: sedimentary rocks

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/syq-esj-snx
title: Stromatolite

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.6084/m9.figshare.28138808.v1
title: <b>The Role of Classroom Climate, Self-Efficacy, and Gender in Music Aesthetic Literacy Among Non-Music Major Students</b>

description: Data from non-music major students
DOI: 10.3535/jrd-96m-05w
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/ngk-vw3-0m7
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/dee-c17-h4r
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/r6c-j37-zzd
title: Chert

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.6084/m9.figshare.28079018.v1
title: Data for "Quantifying the Impact of Environmental Factors the Methane Point-Source Emission Algorithm: A Case Study of HJ-2A/B"

description: The intermediate data in the paper corresponds to the entire process in this paper and can be used for viewing
DOI: 10.17632/kd5p7krfgc
title: Data for: Observer-based Fault Detection for Underwater Thrusters with Applications in Jiaolong HOV
DOI: 10.3535/0g8-ae4-1qr
title: Devononchus concinnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/r8c-zyj-ky6
title: Conglomerate

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/wmh-tkr-r8n
title: Devononchus concinnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.6084/m9.figshare.1415744.v1
title: S5 Table. The optical density (OD) at 590 nm change with incubation time

description: The optical density (OD) at 590 nm change with incubation time
DOI: 10.3535/771-cby-yv4
title: Devononchus concinnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/9bf-dne-f6g
title: Ooid-limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/5gt-vf6-x3d
title: Tryplasma

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/erl-pt7-sby
title: †Skenidioides Schuchert & Cooper, 1931

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/sby-agf-kzy
title: Devononchus concinnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/tmr-lcq-4e1
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/a0b-0hf-t1b
title: †Dicoelosia King, 1850

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/pv2-f5n-7qd
title: Cyathopodium Verrill, 1868

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/hh2-qd5-ghm
title: †Dalmanella Hall & Clarke, 1892

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/svb-6z7-f9c
title: †Dalmanellidae Schuchert, 1913

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/c9j-8pz-zpv
title: †Skenidioides Schuchert & Cooper, 1931

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/s1a-j0d-vwp
title: Porifera Grant, 1836

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/6n0-296-m3p
title: Brachiopoda Duméril, 1805

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/cq4-xnm-xhx
title: glauconitic limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/t4g-mk3-1m9
title: Devononchus concinnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/gtk-a64-dk4
title: Ooid-limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/hnp-6jf-wvv
title: Ooid-limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/30z-880-0hv
title: Zoophycos

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/n30-ewn-qxl
title: †Protochonetes Muir-Wood, 1962

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/87e-rm0-t1g
title: limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/8c7-my3-ty3
title: Ooid-limestone

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/320-1yl-rgl
title: †Dalmanellidae Schuchert, 1913

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/zsp-jq7-8ef
title: Pinaceocladichnus

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/5gn-gen-tvd
title: Porifera Grant, 1836

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.
DOI: 10.3535/6gk-xjt-3ek
title: Porifera Grant, 1836

description: Digital Specimen for the physical specimen hosted at Tallinn University of Technology.