DOI: 10.5061/dryad.6djh9w1f2
title: Genetic diversity and population genetic structure from different host populations of <em>Spodoptera litura</em> based on microsatellite markers

description: Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is an agricultural
 pest with a wide distribution range and many host plants, which can cause
 serious economic losses. In this study, 157 samples were collected from 8
 hosts (tobacco, soybean, taro, peanut, chili, sweet potatoes, corn, and
 sorrel) in Fuli Town, Fuchuan County, Hezhou City, Guangxi Zhuang
 Autonomous Region. Seven microsatellite loci were used to analyze the
 genetic diversity, genetic structure, and genetic differentiation of these
 populations. The results showed that the number of alleles (Na) of 8
 populations ranged from 2.43 to 6.14, the average of polymorphism
 information content (PIC) was 0.4574, the mean of observed heterozygosity
 (HO) and expected heterozygosity (HE) were 0.4926 and 0.5693,
 respectively. The genetic diversity of different host populations was
 high. Based on the Bayesian clustering and NJ phylogenetic tree of genetic
 structure, it was shown that there were certain genetic structures among
 different host populations, which could be roughly divided into four
 genetic groups. The fixed coefficient (FST) between populations ranged
 from 0.010 to 0.208, with low to highly differentiated populations as a
 whole. This study is helpful to understand the occurrence regularity of
 Spodoptera litura from different host plants, and provides a theoretical
 basis for the reasonable adjustment of crop layout in the field and the
 establishment of a corresponding comprehensive control system.
description: # Genetic diversity and population genetic structure from different host
 populations of *Spodoptera litura* based on microsatellite markers Dataset
 DOI: [10.5061/dryad.6djh9w1f2](10.5061/dryad.6djh9w1f2) ## Description of
 the data and file structure These data are microsatellite results from 157
 individuals of eight host populations of *Spodoptera litura*. The samples
 of different hosts were collected from Fuli Town, Fuchuan County, Hezhou
 City, Guangxi Zhuang Autonomous Region (111°23´E, 24°53´N), and a total of
 157 larvae of 8-24 were taken from each host (Table 1), which were tobacco
 (YC), soybean (DD), taro (XY), peanut (HS), chili (LJ), sweet potato (XY),
 corn (YM), and sorrel (SM) populations. Genomic DNA was extracted from the
 head of each larva using Ezup Column Animal Genomic DNA Purification Kit
 (Shanghai, China) according to the manufacturer’s instructions. 157
 samples were amplified by PCR using seven pairs of microsatellite primers
 (CWM−1, CWM−4, CWM−5, CWM−6, CWM−7, CWM−8, and CWM−9) (Wu et al., 2019).
 PCR reaction 25 μL: 2×Taq PCR Master Mix 13 μL, DNA template 1 μL, forward
 and reverse primers 1 μL each, ddH2O 9 μL. Amplification program:
 pre-denaturation at 95°C for 4 min, denaturation at 95°C for 30 s,
 followed by 30 cycles of 30 s at 94°C, annealing at 46-58°C for 30 s, and
 finally extension at 72°C for 7 min. The PCR products were qualified by
 electrophoresis and sent to Sangon Biotech (Shanghai, China) for
 sequencing. ### Files and variables #### File: DATA1.xlsx and DATA2.xlsx
 (The original data of the two files is the same, but the software used and
 the results are different, so the required data formats are not the same）
 **Description:** POP refers to population: There are a total of eight host
 populations, namely: tobacco, soybean, taro, peanut, chili, sweet
 potatoes, corn, and sorrel. Samples refer to all individuals from
 different host populations: 24 from the tobacco population, 24 from the
 taro population, 24 from the peanut population, 23 from the sweet potato
 population, 20 from the sorrel population, 20 from the corn population, 14
 from the soybean population, and 8 from the chili population. There are a
 total of 7 microsatellite primers, namely: CWM−1, CWM−4, CWM−5, CWM−6,
 CWM−7, CWM−8, and CWM−9. ##### Variables * A result with a value of 0
 indicates that no peak value has been read at this position, and the
 result is 0. * *n*, sample size; *N*A, number of alleles; *H*O, observed
 heterogeneity; *H*E, expected heterogeneity; PIC, polymorphism information
 content; *I*, Shannon’s index; *F*IS, inbreeding coefficient. ##
 Code/software The number of alleles (*N*a), polymorphism information
 content (PIC), and Shannon’s index (*I*) were assessed using GenAlEx 6.5
 (Peakall & Smouse, 2012) and Microsatellite Tools (Trinity College
 Dublin, Ireland). Observed heterogeneity (*H*O), expected heterozygosity
 (*H*E), and inbreeding coefficient (*F*IS) were estimated using the
 *hierfstat *R package (Goudet, 2005). The analysis of molecular variance
 (AMOVA) was calculated by ARLEQUIN 3.5 (Excoffier & Lischer, 2010) to
 assess the proportional distribution of genetic variation among
 populations, individuals, and groups, and to estimate the fixed
 coefficient between populations (*F*ST). The genetic structure of
 populations was analyzed based on Bayesian clustering and phylogenetic
 tree, using STRUCTURE 2.3.4 (Pritchard et al., 2000) and MEGA 6.0 (Tamura
 et al., 2013), respectively.