cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.1c59zw3v4
title: Trophic complexity alters the diversity–multifunctionality relationship in experimental grassland mesocosms

description: Plant diversity has a positive influence on the number of ecosystem
 functions maintained simultaneously by a community, or multifunctionality.
 While the presence of multiple trophic levels, or trophic complexity,
 affects individual functions, the effect of trophic complexity on the
 diversity-multifunctionality relationship is less well known. To address
 this issue, we tested whether the independent or simultaneous manipulation
 of both plant diversity and trophic complexity impacted multifunctionality
 using a mecocosm experiment from Cedar Creek, Minnesota, USA. Our analyses
 revealed that neither plant diversity nor trophic complexity had
 significant effects on single functions, but trophic complexity altered
 the diversity-multifunctionality relationship in two key ways: it lowered
 the maximum strength of the diversity-multifunctionality effect and it
 resulted shifted the relationship between increasing diversity and
 multifunctionality from positive to negative at lower function thresholds.
 Our findings suggest that declines in trophic complexity will further
 reduce the capacity of ecosystems to maintain multifunctionality than
 expected from plant biodiversity loss.
description: This dataset was collected in a mesocosm experiment at Cedar
 Creek. The experiment was run from the year 2000-2002. The functional and
 phylogenetic diversity columns were added later using species trait values
 from the TRY dataset and a super tree from Zanne et al 2014
 respectively.
description: bef_manuscript.csv is the main file with the community level
 values of four different ecosystem functions as well as the experimental
 treatments. bef_manuscript_metadata.csv is the metadata
 file for this dataset explaining the different columns.
 plant_species.csv is the file detailing the planted species
 composition in each community (row of the main file) maintained during the
 experiment. multifun_clean.R is the R code used for all
 analyses in the manuscript.
DOI: 10.48689/9282b973-5192-4562-a452-48c47dd7acb7
title: Chemistry and Biology monitored throughout the Capital Regional District, 1974-2012

description: CRD staff monitor levels of contamination in storm drain pipes, creeks, harbours and the nearshore marine environment at over 500 locations.
DOI: 10.48689/80c069d6-4cef-4d9f-91e8-077fc6a8c0ec
title: Pacific Estuary Conservation Program Rankings

description: The PECP is a Pacific Birds program that aims to coordinate efforts to secure and enhance estuary habitats with high ecological value along the coast. Partners include Environment and Climate Change Canada, Ducks Unlimited Canada, BC Ministry of Forests, Lands, Natural Resource Operations and Rural Development, BC Ministry of Environment and Climate Change Strategy, the Habitat Conservation Trust Foundation, the Nature Conservancy of Canada and The Nature Trust of British Columbia. Data from the BC Coastal Waterbird Survey of Birds Canada significantly contributed to the report. The goal is to provide an objective, BC-wide assessment of estuary occurrence and ecological importance to waterbirds. The first report was published in 2007. In this 2019 update, estuaries across the BC coast were identified, mapped, and ranked using GIS tools and standardized criteria. The ranking process was improved from earlier versions by updating or introducing new variables, including estuary size, species rarity, waterbird density, herring spawn, and fish escapement. The end product is a spatially explicit regional overview and prioritization of estuaries for conservation action.
DOI: 10.48689/00bc242d-9145-464b-bb92-c1d0eb1f469a
title: RMIS Salmonid Release and Recovery Data, and Hatchery Locations in the NE Pacific, 2019

description: The attached hatchery location and salmonid release and recovery data were obtained from the Regional Mark Processing Center (RMPC) website. RMPC provides essential services to international, state, federal, and tribal fisheries organizations involved in marking anadromous salmonids throughout the northeast Pacific region. These services include regional coordination of some tagging and fin marking programs, maintenance of databases for Coded Wire Tag Releases, Recoveries, and Locations, as well as the dissemination of reports of these data in electronic or printed form when requested. These databases are known collectively as the Regional Mark Information System (RMIS)
DOI: 10.5061/dryad.866t1g1q9
title: Data from: Rapid post-fire re-assembly of species-rich bryophyte communities in Afroalpine heathlands

description: Questions: In some fire-prone ecosystems, bryophytes play a crucial role
 by providing the surface fuel that controls the fire-return interval.
 Afroalpine heathlands are such an ecosystem, yet almost nothing is known
 about the bryophytes in this system. We do not know the level of species
 richness, or if there is a successive accumulation of species over time,
 or if some species are adapted to specific phases along the successional
 gradient, for example early-successional species sensitive to competition.
 Location: Afroalpine heathlands in Ethiopia Methods: We made an inventory
 of all bryophytes in 48 plots of 5 x 5 m, distributed along a
 chronosequence from 1 to 25 years post-fire. The heathlands are located
 around 3500-3800 m asl, and are managed by traditional pasture burning
 with fire intervals of around 8-20 years. Results: We found in total 111
 taxa of bryophytes. Post-fire mortality was almost 100%. The youngest
 plots had only a few cosmopolitan species often found after fire. Species
 richness increased monotonically and seemed to start levelling off at
 around 15 years after fire, when many plots had around 30 species and a
 high cover of Breutelia diffracta, which is a key ground-living species,
 important as surface fuel. Most species were found with sporophytes, a
 pattern even stronger for the most frequent species. Conclusions:
 Interestingly, bryophyte diversity is already remarkably high by only 15
 years after total eradication. The relatively slow accumulation of species
 in the first years suggest that dispersal in space, and not time, is the
 major mechanism by which sites regain their diversity (i.e. spore banks
 play a small role). This indicates that the high species richness is built
 up through colonization from surrounding heathlands, and perhaps also from
 higher altitude alpine grasslands and lower altitude forests, and that the
 bryophyte diversity in this system is maintained by the traditional fire
 and grazing management.
description: 48 sites with a 5 x 5 m plot in each site were inventoried for
 all bryophytes. A number of environmental and explanatory variables were
 collected in each plot. See the methods section of the paper and the
 ReadMe-file for more information.
description: See further the Paper, Supplementary information and the ReadMe
 file.
DOI: 10.48689/4f178c67-800f-48e4-8c16-aeb5ca10e640
title: Number, Size Composition and Food of Larval and Juvenile Fish Caught with a Two-Boat Surface Trawl in the Strait of Georgia and Saanich Inlet, 1968-1973

description: During the Fraser Plume Study that began in the 1960s, larval and juvenile fishes were caught by a trawl net towed between two boats in the Strait of Georgia during spring and summer from 1966 to 1969 and in 1973. While the data obtained from sampling in 1966 and 1967 were published in the Manuscript Report Series of the Fisheries Research Board of Canada, none of the data collected in 1968, 1969 and 1973 were published. A volunteer effort led to the resurrection of these data from magnetic tapes, formerly stored in the electronic archives of the Pacific Biological Station. The data included station locations, times and dates, sampling effort, fish catches, lengths and body weights of individual fish taken from subsamples of the catch. Quantitative measures of diet, total stomach contents, prey species identification and prey size were available only for the first cruise in 1968 whereas stomach content data for 1966 and 1967 had been published shortly after they were collected.
DOI: 10.48689/7cda9924-dc25-45af-84d1-592fb9de67f8
title: Cowichan Estuary Eelgrass Habitat Suitability Model - Water Depth

description: The Cowichan Estuary Restoration Project is a multi-year project being conducted by Cowichan Tribes to help enhance the recovery of Cowichan Chinook salmon stocks in the Salish Sea. The objective of this model was to use existing information to identify areas within the Cowichan estuary that may be suitable to support eelgrass habitat. This raster layer presents the results of the water depth layer as it pertains to eelgrass suitability, using elevation data derived from Northwest Hydraulic Inc.'s hydrological and geomorphological study of the estuary in 2018.
DOI: 10.5061/dryad.z8w9ghxbx
title: Exercise training has morph-specific effects on telomere, body condition and growth dynamics in a color-polymorphic lizard

description: Alternative reproductive tactics (ARTs) are correlated suites of sexually
 selected traits that are likely to impose differential physiological costs
 on different individuals. While moderate activity might be beneficial,
 animals living in the wild often work at the margins of their resources
 and performance limits. Individuals using ARTs may have divergent
 capacities for activity. When pushed beyond their respective capacities,
 they may experience condition loss, oxidative stress, and molecular damage
 that must be repaired with limited resources. We used the Australian
 painted dragon lizard that exhibits color-polymorphism as a model to
 experimentally test the effect of exercise on body condition, growth,
 reactive oxygen species (ROS), and telomere dynamics, a potential marker
 of stress and aging and a correlate of longevity. For most males, ROS
 tended to be lower with greater exercise; however, males with yellow
 throat patches, or bibs, had higher ROS than non-bibbed males. At the
 highest level of exercise, bibbed males exhibited telomere loss, while
 non-bibbed males gained telomere length; the opposite pattern was observed
 in the no-exercise controls. Growth was positively related to food intake
 but negatively correlated with telomere length at the end of the
 experiment. Body condition was not related to food intake but was
 positively correlated with increases in telomere length. These results,
 along with our previous work, suggest that aggressive, territory holding,
 bibbed males suffer physiological costs that may reduce longevity compared
 to non-bibbed males with superior postcopulatory traits.
DOI: 10.48689/c198358b-6a06-4496-8eb2-8e94474a3592
title: Forage fish habitat monitoring in the Vancouver Island and Gulf Island region by MABRRI, 2017+

description: To date, forage fish spawning habitat monitoring has occurred along the southeastern coast of Vancouver Island, as well as a few Gulf Islands; the areas currently being monitored include: Bowser and Qualicum Bay, Gabriola Island, Ladysmith, Lantzville, Maple Bay, Nanaimo, Nanoose Bay, Parksville, Qualicum Beach, and Thetis Island. Each region has a varying number of beaches sampled, based on beach characteristics, including sediment composition, beach exposure, and general site characteristics. A variety of information is collected from each beach, including: who was sampling, when it was sampled, the organization that funded the sampling, the location of the site, recent high tide information, the tidal elevation from which the sample is taken, the current weather conditions, water temperature, any recent storm events, if there is evidence of sea weed harvesting, the direction (and bearing) in which the beach faces, the slope of the beach, the beaches exposure (i.e. the greatest distance wind can generate waves from across the straight without being disrupted), the sediment composition, the integrity of the area directly above and surrounding the site, the width and length of suitable sediment, and how shaded the site is. Once all of the information is recorded, a sediment sample is taken from area of interest, it is later processed down to a reasonable size that can be analyzed under a dissecting microscope to determine if there are eggs detected in the sample. All of this information is recorded on a field information sheet, which is transcribed into a database.
DOI: 10.48689/5163a55d-a49f-407a-a954-9df878b5f6fa
title: Appendix B: Development of a Hydrodynamic Model of Cowichan-Koksilah River Estuary and Cowichan Bay

description: The Cowichan Estuary Restoration Project is a multi-year project being conducted by Cowichan Tribes to help enhance the recovery of Cowichan Chinook salmon stocks in the Salish Sea. Northwest Hydraulic Consultants Ltd. (NHC) was hired by Cowichan Tribes (CT) to conduct geomorphic studies, field investigations and numerical modelling in support of the project. The purpose of these studies was to provide an overview of the watershed, river system, delta and oceanographic conditions in Cowichan Bay, document changes to the physical characteristics on the tidal flats, summarize the key boundary conditions that govern the physical character of the delta and estuary, and carry out field measurements and hydraulic modelling to characterize the flow and sediment transport characteristics in the estuary. This appendix describes the hydrodynamic model including the creation of a Digital Elevation Model (DEM), model grid and bathymetry, boundary conditions and validation, and results.
DOI: 10.48689/6b05c683-10f5-44fe-9983-7914e5cf0c72
title: Digital elevation model for the Salish Sea at a resolution of 80 metres

description: The integrated bathymetry and topgraphy of the Salish Sea, comprising the entrance and channel of Juan de Fuca Strait, Puget Sound, Strait of Georgia, and significant included Inlets. It uses data primarily from NOAA websites, but data for Strait of Georgia and environs is from the Canadian Hydrographic Service. Much of the data is recent multibeam data. The underlying model is a GeoTiff, which is a raster GeoServer layer. Possible outputs are thus KML for Google earth, Open Layers, and WMS layers.
DOI: 10.48689/8fdb3a11-64e4-4110-83c6-a0d111de7980
title: Ancient Murrelet Colonies

description: Active and historic Ancient Murrelet (Synthliboramphus antiquus) colonies. Raw Parks Canada data used in this feature can be found in the feature: Parks Canada (Haida Gwaii) seabird datasets.
DOI: 10.48689/6e973752-1768-4b0d-bbe8-73e33913eb3c
title: Dungeness Crab- Commercial

description: The Dungeness Crab catch and effort grid dataset has been assembled directly from the Shellfish Stock Assessment harvest log database located at the Pacific Biological Station (PBS). The dataset has been modified to meet the sensitive nature of the data as per the Access to Information Act20(1)(b,c); which specifies that Fisheries and Oceans Canada will not disclose to a third party confidential information that may prejudice the competitive position of the fisher, in which fishing location has been identified as one of the entities to be protected. There was a 4km x 4km grid used in the desensitization process as well information was limited to total Effort (# traps and soak time) and total Catch (calculated lbs and number of pieces). The dataset is comprised of an aggregate of only dungeness and specifically targets the 2000-2004 fishing seasons.
DOI: 10.5061/dryad.t1g1jwt20
title: Systematic dissection of transcriptional regulatory networks by genome-scale and single-cell CRISPR screens

description: Millions of putative transcriptional regulatory elements (TREs) have been
 cataloged in the human genome, yet their functional relevance in specific
 pathophysiological settings remains to be determined. This is critical to
 understand how oncogenic transcription factors (TFs) engage specific TREs
 to impose transcriptional programs underlying malignant phenotypes. Here,
 we combine cutting edge CRISPR screens and epigenomic profiling to
 functionally survey ≈15,000 TREs engaged by estrogen receptor (ER). We
 show that ER exerts its oncogenic role in breast cancer by engaging TREs
 enriched in GATA3, TFAP2C, and H3K27Ac signal. These TREs control critical
 downstream TFs, among which TFAP2C plays an essential role in ER-driven
 cell proliferation. Together, our work reveals novel insights into a
 critical oncogenic transcription program and provides a framework to map
 regulatory networks, enabling to dissect the function of the noncoding
 genome of cancer cells.
description: ChIP-seq was performed as previously described (Galli, G.G., et
 al., Mol Cell, 2015. 60(2): p. 328-37). Briefly, cells were cross-linked
 in 1% formaldehyde for 10 minutes at room temperature after which the
 reaction was stopped by addition of 0.125M glycine. Cells were lysed and
 harvested in ChIP buffer (100 mM Tris at pH=8.6, 0.3% SDS, 1.7% Triton
 X-100, and 5 mM EDTA) and the chromatin disrupted by sonication using
 a EpiShear sonicator (Active Motif) to obtain fragments of average 200-500
 bp in size. Chromatin extracts were incubated for 16 hours with the
 following antibodies: ER (Cell Signaling, 13258), FOXA1 (Cell
 Signaling, 58613), GATA3 (Cell Signaling, 5852), CTCF (Cell Signaling,
 2899), H3K27ac (Cell Signaling, 8173) and H3K4me1 (Cell Signaling,
 5326). Immunoprecipitated complexes were recovered using Protein
 G Dynabeads (Invitrogen) and DNA was recovered by reverse-crosslinking and
 purified using SPRI Select beads (Beckman Coulter). Libraries
 for ChIP-sequencing were generated using Ovation® Ultralow Library System
 V2 (NuGEN) and barcodes were added using NEBNext Multiplex Oligos for
 Illumina (NEB, Index Primers Set 1) according to the manufacturer’s
 recommendation. All next-generation sequencing experiments were run on a
 HiSeq2500 (Illumina). Fastq files were aligned to a human reference genome
 (hg38) using bowtie2 v2.3.4.1 and sorted using samtools v1.8. Duplicates
 were marked and removed using Picard MarkDuplicates v2.18.7
 (http://broadinstitute.github.io/picard), and low quality mapped reads
 (below 20) were removed using samtools. Samtools view was used to retain
 reads mapping to human chromosomes and to discard reads mapping
 to chrM for ATAC-seq samples. Deeptools was used to generate
 RPKM-normalized bigwig files.
DOI: 10.48689/23ef283b-49c1-4ab3-b794-464af6313c63
title: British Columbia Coastal Waterbirds Survey

description: Used for deriving trends in waterbird populations and locating important staging areas
DOI: 10.48689/36633c4e-2e19-4a09-a403-cc67955041f8
title: 1977_Populations, biomass and food habits of ducks on the Fraser Delta intertidal area BC

description: The Fraser Delta intertidal region consists chiefly o f three extensive tidal flats (Figure 1): Sturgeon and Roberts Banks, together also referred to as the Fraser Delta foreshore, and Boundary Bay. These areas constitute the largest wintering grounds for ducks along the British Columbia coast, and the Canadian Wildlife Service have made aerial surveys o f ducks there for a decade. Food studies on dabbling ducks have been conducted in estuarine marshes o f Sturgeon and Roberts Banks by Burgess (1970), but in 1974, the first author initiated the first comprehensive food study o f diving ducks on the tidal flats. An attempt is made here to present the population and food data as a stepping stone for further ornithological investigations as well as for environmental assessment.
DOI: 10.5061/dryad.0k6djhb0h
title: Comparison of foraging tool use in two species of myrmicine ants (Hymenoptera: Formicidae)

description: The tool-using behavior of Aphaenogaster species has been well studied in
 the past few years, however, our knowledge of other myrmicine ants is
 rather limited. The aim of the present study was to compare the tool-using
 behavior of two closely related myrmicine ants with different feeding
 behaviors: Aphaenogaster subterranea, an omnivorous species, and Messor
 structor, a mainly granivorous species, which is also known to use tools
 when feeding on liquids. We tested the hypothesis that due to the
 difference in diet, the tool-using behavior differs in these two species
 being more complex and dynamic in A. subterranea than in
 M. structor. Consistent with our hypothesis, the foraging workers
 of M. structor dropped and retrieved significantly less tools
 into/from honey-water baits than A. subterranea, and, contrary to
 the latter species, showed no preference towards any specific tools. In
 addition, tool dropping was significantly slower in M. structor,
 and the dropping and retrieving of tools began significantly later than in
 A. subterranea. For seed-harvester ants, the ability to
 effectively transport liquid food via tools may not be as significant as
 for Aphaenogaster species that regularly utilize and compete for liquid
 food sources. Dropping tools into liquids, however, may still be adaptive
 for seed-harvester species as a means to supplement diet with liquid food
 during periods of seed shortage and also to serve as a defensive behavior
 against threatening liquids close to the nest.
DOI: 10.48689/28058708-b9bc-4471-80fb-5770d0b7bf65
title: Preliminary study of the influence of current velocities on estuarine benthos, especially Anisogammarus confervicolus, in the Fraser River estuary (South Arm)

description: Field studies in the lower Fraser River (South Arm) were conducted during July to August 1976 to provide data on the abundance of benthic species at habitats subjected to different river flow regimes. Freshwater organisms, especially oligochaetes and chironomids, dominated quadrat samples in the Ladner marsh. Oligochaetes and chironomids were most common in grab samples in Ladner and Sea Reaches, and largest numbers were founda t stations characterized by leaf debris. An isopod (Gnorimosphaeroma oregonensis), the amphipods Corophiun spinicorne and Anisogamnarus confervicolus, and chironomid larvae dominated drift samplers. Benthic organisms were more abundant in side channels where current velocities were reduced. A reconnaissance survey of Steveston Island showed that benthic organisms have colonized some habitats of this partially man-made island. Preliminary laboratory studies with the amphipod Anisogammarus aonfervicolus