cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22551220
title: Insights into the regulation of MAP3K1 in hair follicle stem cells by transcriptome analysis

description: MAP3K1 is a significant member of the MAPK family, and its expressed MEKK1 protein has a wide range of biological activities and is an essential node in the MAPK signaling pathway. A significant number of studies have revealed that MAP3K1 plays a complicated function in the control of cell proliferation, apoptosis, invasion and movement, participates in the regulation of the immune system, and plays an important role in wound healing, tumorigenesis and other processes. In this study, we looked at the involvement of MAP3K1 in the control of hair follicle stem cells (HFSCs). Overexpression of MAP3K1 significantly promoted the proliferation of HFSCs by inhibiting apoptosis and promoting the transition from S phase to G2 phase. The transcriptome identified 189 (MAP3K1_OE) and 414 (MAP3K1_sh) differential genes. The two pathways with the most significant enrichment of differentially expressed genes were the IL-17 signaling pathway and TNF signaling pathway, and the significantly enriched terms in the GO enrichment analysis involved regulation of response of external stimulus, inflammatory and cytokine. Indicate that MAP3K1 can function as a promoting factor in HFSCs through the induction of cell cycle transition from S phase to G2 phase can inhibition apoptosis by mediating crosstalk among several pathways and cytokines.HIGHLIGHTSAbnormal MAP3K1 expression in hair follicle stem cells (HFSCs) can impair HFSC proliferation and apoptosis.MAP3K1 controls hair follicle stem cell proliferation via modulating cell apoptosis and the ratio of cells in S phase/G2 phase.The differential genes shared by MAP3K1_sh and MAP3K1_OE are enriched in GO terms such as inflammation, adipocyte differentiation, acute inflammation, and so on. Abnormal MAP3K1 expression in hair follicle stem cells (HFSCs) can impair HFSC proliferation and apoptosis. MAP3K1 controls hair follicle stem cell proliferation via modulating cell apoptosis and the ratio of cells in S phase/G2 phase. The differential genes shared by MAP3K1_sh and MAP3K1_OE are enriched in GO terms such as inflammation, adipocyte differentiation, acute inflammation, and so on.
DOI: 10.6084/m9.figshare.22551220.v1
title: Insights into the regulation of MAP3K1 in hair follicle stem cells by transcriptome analysis

description: MAP3K1 is a significant member of the MAPK family, and its expressed MEKK1 protein has a wide range of biological activities and is an essential node in the MAPK signaling pathway. A significant number of studies have revealed that MAP3K1 plays a complicated function in the control of cell proliferation, apoptosis, invasion and movement, participates in the regulation of the immune system, and plays an important role in wound healing, tumorigenesis and other processes. In this study, we looked at the involvement of MAP3K1 in the control of hair follicle stem cells (HFSCs). Overexpression of MAP3K1 significantly promoted the proliferation of HFSCs by inhibiting apoptosis and promoting the transition from S phase to G2 phase. The transcriptome identified 189 (MAP3K1_OE) and 414 (MAP3K1_sh) differential genes. The two pathways with the most significant enrichment of differentially expressed genes were the IL-17 signaling pathway and TNF signaling pathway, and the significantly enriched terms in the GO enrichment analysis involved regulation of response of external stimulus, inflammatory and cytokine. Indicate that MAP3K1 can function as a promoting factor in HFSCs through the induction of cell cycle transition from S phase to G2 phase can inhibition apoptosis by mediating crosstalk among several pathways and cytokines.HIGHLIGHTSAbnormal MAP3K1 expression in hair follicle stem cells (HFSCs) can impair HFSC proliferation and apoptosis.MAP3K1 controls hair follicle stem cell proliferation via modulating cell apoptosis and the ratio of cells in S phase/G2 phase.The differential genes shared by MAP3K1_sh and MAP3K1_OE are enriched in GO terms such as inflammation, adipocyte differentiation, acute inflammation, and so on. Abnormal MAP3K1 expression in hair follicle stem cells (HFSCs) can impair HFSC proliferation and apoptosis. MAP3K1 controls hair follicle stem cell proliferation via modulating cell apoptosis and the ratio of cells in S phase/G2 phase. The differential genes shared by MAP3K1_sh and MAP3K1_OE are enriched in GO terms such as inflammation, adipocyte differentiation, acute inflammation, and so on.
DOI: 10.6084/m9.figshare.22551220.v2
title: Insights into the regulation of MAP3K1 in hair follicle stem cells by transcriptome analysis

description: MAP3K1 is a significant member of the MAPK family, and its expressed MEKK1 protein has a wide range of biological activities and is an essential node in the MAPK signaling pathway. A significant number of studies have revealed that MAP3K1 plays a complicated function in the control of cell proliferation, apoptosis, invasion and movement, participates in the regulation of the immune system, and plays an important role in wound healing, tumorigenesis and other processes. In this study, we looked at the involvement of MAP3K1 in the control of hair follicle stem cells (HFSCs). Overexpression of MAP3K1 significantly promoted the proliferation of HFSCs by inhibiting apoptosis and promoting the transition from S phase to G2 phase. The transcriptome identified 189 (MAP3K1_OE) and 414 (MAP3K1_sh) differential genes. The two pathways with the most significant enrichment of differentially expressed genes were the IL-17 signaling pathway and TNF signaling pathway, and the significantly enriched terms in the GO enrichment analysis involved regulation of response of external stimulus, inflammatory and cytokine. Indicate that MAP3K1 can function as a promoting factor in HFSCs through the induction of cell cycle transition from S phase to G2 phase can inhibition apoptosis by mediating crosstalk among several pathways and cytokines.HIGHLIGHTSAbnormal MAP3K1 expression in hair follicle stem cells (HFSCs) can impair HFSC proliferation and apoptosis.MAP3K1 controls hair follicle stem cell proliferation via modulating cell apoptosis and the ratio of cells in S phase/G2 phase.The differential genes shared by MAP3K1_sh and MAP3K1_OE are enriched in GO terms such as inflammation, adipocyte differentiation, acute inflammation, and so on. Abnormal MAP3K1 expression in hair follicle stem cells (HFSCs) can impair HFSC proliferation and apoptosis. MAP3K1 controls hair follicle stem cell proliferation via modulating cell apoptosis and the ratio of cells in S phase/G2 phase. The differential genes shared by MAP3K1_sh and MAP3K1_OE are enriched in GO terms such as inflammation, adipocyte differentiation, acute inflammation, and so on.
DOI: 10.6084/m9.figshare.9968414
title: Research data.xlsx

description: This dataset include whole rock element concentration, iron isotopic composition, and mineral major element
compositions of peridotite from the Yushigou ophiolite in the North Qilian orogen, northern Tibet.
DOI: 10.6084/m9.figshare.6822698
title: Effectiveness and safety of dual influenza and pneumococcal vaccination versus separate administration or no vaccination in older adults: a meta-analysis

description: <b>Background:</b> The advantages of dual pneumococcal and influenza vaccination in older adults have not been clarified and controversy remains regarding their optimal use. <b>Methods:</b> This meta-analysis was conducted on 25 January 2018 using PubMed, the Cochrane Library, and Embase databases. Seventeen studies were selected ultimately for meta-analysis using a multi-step approach by two separate authors. Primary outcomes were pneumonia, pneumococcal pneumonia, influenza, hospitalization, and all-cause mortality rates, and the secondary outcome was adverse effects (AEs). <b>Results:</b> The additive preventive effects of dual inﬂuenza and pneumococcal vaccination versus inﬂuenza vaccination alone for pneumonia and death were 15% (95% confidence interval [CI]: 4-24%) and 19% (95% CI: 6-30%), respectively. Compared with pneumococcal vaccination alone, dual influenza and pneumococcal vaccination resulted in a 24% (95% CI: 16-31%) reduction in pneumonia and a 28% (95% CI: 13-40%) reduction in death. Compared with placebo or no vaccination, the effectiveness of dual vaccination was 29% (95% CI: 14-42%) for pneumonia, 38% (95% CI: 25-49%) for death, 35% (95% CI: 22-46%) for influenza, and 18% (95% CI: 6-29%) for hospitalization. Both vaccines showed acceptable safety profiles and AEs were mild or moderate. <b>Conclusion:</b> Our findings highlight the importance of concomitant influenza and pneumococcal vaccination in older adults.
DOI: 10.6084/m9.figshare.6822698.v1
title: Effectiveness and safety of dual influenza and pneumococcal vaccination versus separate administration or no vaccination in older adults: a meta-analysis

description: <b>Background:</b> The advantages of dual pneumococcal and influenza vaccination in older adults have not been clarified and controversy remains regarding their optimal use. <b>Methods:</b> This meta-analysis was conducted on 25 January 2018 using PubMed, the Cochrane Library, and Embase databases. Seventeen studies were selected ultimately for meta-analysis using a multi-step approach by two separate authors. Primary outcomes were pneumonia, pneumococcal pneumonia, influenza, hospitalization, and all-cause mortality rates, and the secondary outcome was adverse effects (AEs). <b>Results:</b> The additive preventive effects of dual inﬂuenza and pneumococcal vaccination versus inﬂuenza vaccination alone for pneumonia and death were 15% (95% confidence interval [CI]: 4-24%) and 19% (95% CI: 6-30%), respectively. Compared with pneumococcal vaccination alone, dual influenza and pneumococcal vaccination resulted in a 24% (95% CI: 16-31%) reduction in pneumonia and a 28% (95% CI: 13-40%) reduction in death. Compared with placebo or no vaccination, the effectiveness of dual vaccination was 29% (95% CI: 14-42%) for pneumonia, 38% (95% CI: 25-49%) for death, 35% (95% CI: 22-46%) for influenza, and 18% (95% CI: 6-29%) for hospitalization. Both vaccines showed acceptable safety profiles and AEs were mild or moderate. <b>Conclusion:</b> Our findings highlight the importance of concomitant influenza and pneumococcal vaccination in older adults.
DOI: 10.5281/zenodo.10530509
title: Protocol for certifying entanglement in surface spin systems using a scanning tunneling microscope

description: This folder contains all data for the paper titled "Protocol for certifying entanglement in a surface spin system using a scanning tunneling microscope". See readme file for more information.
DOI: 10.6084/m9.figshare.24648660
title: HiGTS

description: HiGTS is a high-resolution global gridded dataset of human thermal stress indices. Derived using climate variables from ERA5-Land and ERA5, the HiGTS consists of hourly gridded maps of MRT, UTCI, UTCI level, and daily TSD at 0.1° × 0.1° spatial resolution over the global land surface. It spans from January 1, 2000, to December 31, 2023. However, the data in December 2023 was uploaded for demonstration due to repository capacity limitations. The full dataset is available and free to download via the Data Sharing and Service Portal which is built for the release and sharing of data resources of the Big Earth Data Science Engineering Project (CASEarth) launched by the Chinese Academy of Sciences (CAS).
DOI: 10.6084/m9.figshare.22551562
title: Assessment and Selection of Cyanobacterial Strains for CO<sub>2</sub> Mineral Sequestration: Implications for Carbonation Mechanism

description: CO<sub>2</sub> mineral carbonation induced by microalgae is an emerging approach to carbon capture, utilization, and storage (CCUS). Freshwater cyanobacteria are common microalgae in nature that can raise the pH of eutrophic waters and drive the precipitation of carbonate. Still, limited studies have been conducted to evaluate and select appropriate cyanobacteria strains for CCUS. Here we present experimental investigations to compare the capacity of different freshwater cyanobacterial strains in converting CO<sub>2</sub> to carbonates. We compare five cyanobacterial strains by monitoring their growth curves. We examine three metrics, the maximum pH of the solution, hydroxide production capacity, and extracellular polymeric substances (EPS) secretion capacity, to assess the capacity for carbon sequestration. Our results indicate that among the five strains, <i>Microcystis aeruginosa</i> shows the highest pH and EPS content per unit cell number, marking the most significant capacity for CO<sub>2</sub> carbonation. We observe carbonate precipitates as hydromagnesite and dypingite. We suggest the negatively charged, hydrophilic EPS can effectively promote the precipitation of magnesium carbonate and inhibit the precipitation of calcium carbonate. Overall, our approach provides a framework for assessing and selecting cyanobacterial strains for CO<sub>2</sub> carbonation, advancing the understanding of the mechanism of microalgae-induced CO<sub>2</sub> Mg-carbonation from the perspective of EPS surface properties.
DOI: 10.6084/m9.figshare.22551562.v1
title: Assessment and Selection of Cyanobacterial Strains for CO<sub>2</sub> Mineral Sequestration: Implications for Carbonation Mechanism

description: CO<sub>2</sub> mineral carbonation induced by microalgae is an emerging approach to carbon capture, utilization, and storage (CCUS). Freshwater cyanobacteria are common microalgae in nature that can raise the pH of eutrophic waters and drive the precipitation of carbonate. Still, limited studies have been conducted to evaluate and select appropriate cyanobacteria strains for CCUS. Here we present experimental investigations to compare the capacity of different freshwater cyanobacterial strains in converting CO<sub>2</sub> to carbonates. We compare five cyanobacterial strains by monitoring their growth curves. We examine three metrics, the maximum pH of the solution, hydroxide production capacity, and extracellular polymeric substances (EPS) secretion capacity, to assess the capacity for carbon sequestration. Our results indicate that among the five strains, <i>Microcystis aeruginosa</i> shows the highest pH and EPS content per unit cell number, marking the most significant capacity for CO<sub>2</sub> carbonation. We observe carbonate precipitates as hydromagnesite and dypingite. We suggest the negatively charged, hydrophilic EPS can effectively promote the precipitation of magnesium carbonate and inhibit the precipitation of calcium carbonate. Overall, our approach provides a framework for assessing and selecting cyanobacterial strains for CO<sub>2</sub> carbonation, advancing the understanding of the mechanism of microalgae-induced CO<sub>2</sub> Mg-carbonation from the perspective of EPS surface properties.
DOI: 10.6084/m9.figshare.22551573
title: Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury

description: Macroautophagy/autophagy plays a protective role in sepsis-induced liver injury. As a member of class B scavenger receptors, CD36 plays important roles in various disorders, such as atherosclerosis and fatty liver disease. Here we found that the expression of CD36 in hepatocytes was increased in patients and a mouse model with sepsis, accompanied by impaired autophagy flux. Furthermore, hepatocyte <i>cd36</i> knockout (<i>cd36</i>-HKO) markedly improved liver injury and the impairment of autophagosome-lysosome fusion in lipopolysaccharide (LPS)-induced septic mice. <i>Ubqln1</i> (ubiquilin 1) overexpression (OE) in hepatocyte blocked the protective effect of <i>cd36</i>-HKO on LPS-induced liver injury in mice. Mechanistically, with LPS stimulation, CD36 on the plasma membrane was depalmitoylated and distributed to the lysosome, where CD36 acted as a bridge molecule linking UBQLN1 to soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins and hence promoting the proteasomal degradation of SNARE proteins, resulting in fusion impairment. Overall, our data reveal that CD36 is essential for modulating the proteasomal degradation of autophagic SNARE proteins in a UBQLN1-dependent manner. Targeting CD36 in hepatocytes is effective for improving autophagic flux in sepsis and therefore represents a promising therapeutic strategy for clinical treatment of septic liver injury. <b>Abbreviations:</b> AAV8: adeno-associated virus 8; AOSC: acute obstructive suppurative cholangitis; ATP1A1: ATPase, Na+/K+ transporting, alpha 1 polypeptide; CASP3: caspase 3; CASP8: caspase 8; CCL2: chemokine (C-C motif) ligand 2; <i>cd36</i>-HKO: hepatocyte-specific <i>cd36</i> knockout; Co-IP: co-immunoprecipitation; CQ: chloroquine; Cys: cysteine; GOT1: glutamic-oxaloacetic transaminase 1, soluble; GPT: glutamic–pyruvic transaminase, soluble; IL1B: interleukin 1 beta; IL6: interleukin 6; KO: knockout; LAMP1: lysosomal associated membrane protein 1; LDH, lactate dehydrogenase; LPS: lipopolysaccharide; LYPLA1: lysophospholipase 1; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; OE: overexpression; qPCR: quantitative polymerase chain reaction; SNAP29: synaptosome associated protein 29; SNARE: soluble N-ethylmaleimide-sensitive factor attachment protein receptor; SQSTM1/p62: sequestosome 1; STX17: syntaxin 17; TNF: tumor necrosis factor; TRIM: tripartite motif-containing; UBA: ubiquitin-associated; UBL: ubiquitin-like; UBQLN: ubiquilin; VAMP8: vesicle associated membrane protein 8; WT: wild-type.
DOI: 10.6084/m9.figshare.22551573.v1
title: Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury

description: Macroautophagy/autophagy plays a protective role in sepsis-induced liver injury. As a member of class B scavenger receptors, CD36 plays important roles in various disorders, such as atherosclerosis and fatty liver disease. Here we found that the expression of CD36 in hepatocytes was increased in patients and a mouse model with sepsis, accompanied by impaired autophagy flux. Furthermore, hepatocyte <i>cd36</i> knockout (<i>cd36</i>-HKO) markedly improved liver injury and the impairment of autophagosome-lysosome fusion in lipopolysaccharide (LPS)-induced septic mice. <i>Ubqln1</i> (ubiquilin 1) overexpression (OE) in hepatocyte blocked the protective effect of <i>cd36</i>-HKO on LPS-induced liver injury in mice. Mechanistically, with LPS stimulation, CD36 on the plasma membrane was depalmitoylated and distributed to the lysosome, where CD36 acted as a bridge molecule linking UBQLN1 to soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins and hence promoting the proteasomal degradation of SNARE proteins, resulting in fusion impairment. Overall, our data reveal that CD36 is essential for modulating the proteasomal degradation of autophagic SNARE proteins in a UBQLN1-dependent manner. Targeting CD36 in hepatocytes is effective for improving autophagic flux in sepsis and therefore represents a promising therapeutic strategy for clinical treatment of septic liver injury. <b>Abbreviations:</b> AAV8: adeno-associated virus 8; AOSC: acute obstructive suppurative cholangitis; ATP1A1: ATPase, Na+/K+ transporting, alpha 1 polypeptide; CASP3: caspase 3; CASP8: caspase 8; CCL2: chemokine (C-C motif) ligand 2; <i>cd36</i>-HKO: hepatocyte-specific <i>cd36</i> knockout; Co-IP: co-immunoprecipitation; CQ: chloroquine; Cys: cysteine; GOT1: glutamic-oxaloacetic transaminase 1, soluble; GPT: glutamic–pyruvic transaminase, soluble; IL1B: interleukin 1 beta; IL6: interleukin 6; KO: knockout; LAMP1: lysosomal associated membrane protein 1; LDH, lactate dehydrogenase; LPS: lipopolysaccharide; LYPLA1: lysophospholipase 1; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; OE: overexpression; qPCR: quantitative polymerase chain reaction; SNAP29: synaptosome associated protein 29; SNARE: soluble N-ethylmaleimide-sensitive factor attachment protein receptor; SQSTM1/p62: sequestosome 1; STX17: syntaxin 17; TNF: tumor necrosis factor; TRIM: tripartite motif-containing; UBA: ubiquitin-associated; UBL: ubiquitin-like; UBQLN: ubiquilin; VAMP8: vesicle associated membrane protein 8; WT: wild-type.
DOI: 10.6084/m9.figshare.21502998
title: Toward Optimal Variance Reduction in Online Controlled Experiments

description: We study optimal variance reduction solutions for count and ratio metrics in online controlled experiments. Our methods apply flexible machine learning tools to incorporate covariates that are independent from the treatment but have predictive power for the outcomes, and employ the cross-fitting technique to remove the bias in complex machine learning models. We establish CLT-type asymptotic inference based on our estimators under mild convergence conditions. Our procedures are optimal (efficient) for the corresponding targets as long as the machine learning estimators are consistent, without any requirement for their convergence rates. In complement to the general optimal procedure, we also derive a linear adjustment method for ratio metrics as a special case that is computationally efficient and can flexibly incorporate any pretreatment covariates. We evaluate the proposed variance reduction procedures with comprehensive simulation studies and provide practical suggestions regarding commonly adopted assumptions in computing ratio metrics. When tested on real online experiment data from LinkedIn, the proposed optimal procedure for ratio metrics can reduce up to 80% of variance compared to the standard difference-in-mean estimator and also further reduce up to 30% of variance compared to the CUPED approach by going beyond linearity and incorporating a large number of extra covariates.
DOI: 10.6084/m9.figshare.21502998.v2
title: Toward Optimal Variance Reduction in Online Controlled Experiments

description: We study optimal variance reduction solutions for count and ratio metrics in online controlled experiments. Our methods apply flexible machine learning tools to incorporate covariates that are independent from the treatment but have predictive power for the outcomes, and employ the cross-fitting technique to remove the bias in complex machine learning models. We establish CLT-type asymptotic inference based on our estimators under mild convergence conditions. Our procedures are optimal (efficient) for the corresponding targets as long as the machine learning estimators are consistent, without any requirement for their convergence rates. In complement to the general optimal procedure, we also derive a linear adjustment method for ratio metrics as a special case that is computationally efficient and can flexibly incorporate any pretreatment covariates. We evaluate the proposed variance reduction procedures with comprehensive simulation studies and provide practical suggestions regarding commonly adopted assumptions in computing ratio metrics. When tested on real online experiment data from LinkedIn, the proposed optimal procedure for ratio metrics can reduce up to 80% of variance compared to the standard difference-in-mean estimator and also further reduce up to 30% of variance compared to the CUPED approach by going beyond linearity and incorporating a large number of extra covariates.