cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14446822
title: Cleavage of the vaspin N-terminus releases cell-penetrating peptides that affect early stages of adipogenesis and inhibit lipolysis in mature adipocytes

description: Vaspin expression and function is related to metabolic disorders and comorbidities of obesity. In various cellular and animal models of obesity, diabetes and atherosclerosis vaspin has shown beneficial, protective and/or compensatory action. While testing proteases for inhibition by vaspin, we noticed specific cleavage within the vaspin N-terminus and sequence analysis predicted cell-penetrating activity for the released peptides. These findings raised the question whether these proteolytic peptides exhibit biological activity. We synthesized various N-terminal vaspin peptides to investigate cell-penetrating activity and analyse uptake mechanisms. Focusing on adipocytes, we performed microarray analysis and functional assays to elucidate biological activities of the vaspin–derived peptide, which is released by KLK7 cleavage (vaspin residues 21-30; VaspinN). Our study provides first evidence that proteolytic processing of the vaspin N-terminus releases cell-penetrating and bioactive peptides with effects on adipocyte biology. The VaspinN peptide increased preadipocyte proliferation, interfered with clonal expansion during the early stage of adipogenesis and blunted adrenergic cAMP-signalling, downstream lipolysis as well as insulin signalling in mature adipocytes. Protease-mediated release of functional N-terminal peptides presents an additional facet of vaspin action. Future studies will address the mechanisms underlying the biological activities and clarify, if vaspin-derived peptides may have potential as therapeutic agents for the treatment of metabolic diseases.
DOI: 10.6084/m9.figshare.14046453
title: Association between rs174537 <i>FADS1</i> polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity

description: Fatty acid desaturase 1 (<i>FADS1</i>) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between <i>FADS1</i> variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in <i>FADS1</i> was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. <i>FADS1</i> gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although <i>FADS1</i> gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in <i>FADS1</i> has minimal impact on inflammatory status in obese SAT.
DOI: 10.6084/m9.figshare.14046453.v1
title: Association between rs174537 <i>FADS1</i> polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity

description: Fatty acid desaturase 1 (<i>FADS1</i>) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between <i>FADS1</i> variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in <i>FADS1</i> was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. <i>FADS1</i> gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although <i>FADS1</i> gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in <i>FADS1</i> has minimal impact on inflammatory status in obese SAT.
DOI: 10.6084/m9.figshare.16879210.v1
title: Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice

description: Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.
DOI: 10.6084/m9.figshare.13861550
title: Identification of the transgene insertion site for an adipocyte-specific adiponectin-cre model and characterization of the functional consequences

description: The <i>Adipoq-Cre</i> transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective <i>Adipoq-Cre</i> transgenic mouse, strong genetic linkage between a gene of interest, <i>Adam10</i>, and the <i>Adipoq-Cre</i> transgene was discovered. Whole-genome sequencing of the <i>Adipoq-Cre</i> transgenic mouse model identified the genomic insertion site within the <i>Tbx18</i> gene locus on chromosome 9 and this insertion causes a significant decrease in <i>Tbx18</i> gene expression in adipose tissue. Insertion of genes <i>Kng2, Kng1, Eif4a2</i> and <i>Rfc4</i> also occurred in the <i>Adipoq-Cre</i> transgenic mouse, and these passenger genes may have functional consequences in various tissues.
DOI: 10.6084/m9.figshare.16879210
title: Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice

description: Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.
DOI: 10.6084/m9.figshare.16879246
title: Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes

description: Adipogenesis regulation is crucial for mature adipocyte function. In obesity, a major driver of type 2 diabetes (T2D), this process is disrupted and remains poorly characterized. Here we identified altered DNA methylation profiles in diabetic obese patients, during three adipocytes differentiation stages. We isolated mesenchymal cells from visceral adipose tissue of obese patients with and without T2D to analyse DNA methylation profiles at 0, 3, and 18 days of <i>ex vivo</i> differentiation and documented their impact on gene expression. Methylation and gene expression were analysed with EPIC and Clarion S arrays, respectively. Patients with T2D had epigenetic alterations in all the analysed stages, and these were mainly observed in genes important in adipogenesis, insulin resistance, cell death programming, and immune effector processes. Importantly, at 3 days, we found six-fold more methylated CpG alterations than in the other stages. This is the first study to document epigenetic markers that persist through all three adipogenesis stages and their impact on gene expression, which could be a cellular metabolic memory involved in T2D. Our data provided evidence that, throughout the adipogenesis process, alterations occur in methylation that might impact mature adipocyte function, cause tissue malfunction, and potentially, lead to the development of T2D.
DOI: 10.6084/m9.figshare.13861550.v1
title: Identification of the transgene insertion site for an adipocyte-specific adiponectin-cre model and characterization of the functional consequences

description: The <i>Adipoq-Cre</i> transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective <i>Adipoq-Cre</i> transgenic mouse, strong genetic linkage between a gene of interest, <i>Adam10</i>, and the <i>Adipoq-Cre</i> transgene was discovered. Whole-genome sequencing of the <i>Adipoq-Cre</i> transgenic mouse model identified the genomic insertion site within the <i>Tbx18</i> gene locus on chromosome 9 and this insertion causes a significant decrease in <i>Tbx18</i> gene expression in adipose tissue. Insertion of genes <i>Kng2, Kng1, Eif4a2</i> and <i>Rfc4</i> also occurred in the <i>Adipoq-Cre</i> transgenic mouse, and these passenger genes may have functional consequences in various tissues.
DOI: 10.6084/m9.figshare.16879246.v1
title: Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes

description: Adipogenesis regulation is crucial for mature adipocyte function. In obesity, a major driver of type 2 diabetes (T2D), this process is disrupted and remains poorly characterized. Here we identified altered DNA methylation profiles in diabetic obese patients, during three adipocytes differentiation stages. We isolated mesenchymal cells from visceral adipose tissue of obese patients with and without T2D to analyse DNA methylation profiles at 0, 3, and 18 days of <i>ex vivo</i> differentiation and documented their impact on gene expression. Methylation and gene expression were analysed with EPIC and Clarion S arrays, respectively. Patients with T2D had epigenetic alterations in all the analysed stages, and these were mainly observed in genes important in adipogenesis, insulin resistance, cell death programming, and immune effector processes. Importantly, at 3 days, we found six-fold more methylated CpG alterations than in the other stages. This is the first study to document epigenetic markers that persist through all three adipogenesis stages and their impact on gene expression, which could be a cellular metabolic memory involved in T2D. Our data provided evidence that, throughout the adipogenesis process, alterations occur in methylation that might impact mature adipocyte function, cause tissue malfunction, and potentially, lead to the development of T2D.
DOI: 10.6084/m9.figshare.16611338
title: Different effects of high-fat and high-sucrose diets on the physiology of perivascular adipose tissues of the thoracic and abdominal aorta

description: Vascular diseases such as atherosclerosis and aneurysms are associated with diet. Perivascular adipose tissue (PVAT) was reportedly involved in the regulation of vascular functions. It is suggested that imbalanced diets can cause PVAT inflammation and dysfunction as well as impaired vascular function. However, the association between diets and PVAT are not clearly understood. Here, we showed that a high-fat and a high-sucrose diet affected PVAT at different sites. A high-fat diet induced increased number of large-sized lipid droplets and increased CD (Cluster of differentiation) 68+ macrophage- and monocyte chemotactic protein (MCP)-1-positive areas in the abdominal aortic PVAT (aPVAT). In addition, a high-fat diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the abdominal aorta. In contrast, a high-sucrose diet induced increased number of large-sized lipid droplets, increased CD68+ macrophage- and MCP-1-positive areas, and decreased UCP-1 positive area in the thoracic aortic PVAT (tPVAT). A high-sucrose diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the thoracic aorta. These results could be attributed to the different adipocyte populations in the tPVAT and aPVAT. Our results provide pathological evidence to improve our understanding of the relationship between diet and vascular diseases.
DOI: 10.6084/m9.figshare.16611338.v2
title: Different effects of high-fat and high-sucrose diets on the physiology of perivascular adipose tissues of the thoracic and abdominal aorta

description: Vascular diseases such as atherosclerosis and aneurysms are associated with diet. Perivascular adipose tissue (PVAT) was reportedly involved in the regulation of vascular functions. It is suggested that imbalanced diets can cause PVAT inflammation and dysfunction as well as impaired vascular function. However, the association between diets and PVAT are not clearly understood. Here, we showed that a high-fat and a high-sucrose diet affected PVAT at different sites. A high-fat diet induced increased number of large-sized lipid droplets and increased CD (Cluster of differentiation) 68+ macrophage- and monocyte chemotactic protein (MCP)-1-positive areas in the abdominal aortic PVAT (aPVAT). In addition, a high-fat diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the abdominal aorta. In contrast, a high-sucrose diet induced increased number of large-sized lipid droplets, increased CD68+ macrophage- and MCP-1-positive areas, and decreased UCP-1 positive area in the thoracic aortic PVAT (tPVAT). A high-sucrose diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the thoracic aorta. These results could be attributed to the different adipocyte populations in the tPVAT and aPVAT. Our results provide pathological evidence to improve our understanding of the relationship between diet and vascular diseases.
DOI: 10.6084/m9.figshare.16611338.v1
title: Different effects of high-fat and high-sucrose diets on the physiology of perivascular adipose tissues of the thoracic and abdominal aorta

description: Vascular diseases such as atherosclerosis and aneurysms are associated with diet. Perivascular adipose tissue (PVAT) was reportedly involved in the regulation of vascular functions. It is suggested that imbalanced diets can cause PVAT inflammation and dysfunction as well as impaired vascular function. However, the association between diets and PVAT are not clearly understood. Here, we showed that a high-fat and a high-sucrose diet affected PVAT at different sites. A high-fat diet induced increased number of large-sized lipid droplets and increased CD (Cluster of differentiation) 68+ macrophage- and monocyte chemotactic protein (MCP)-1-positive areas in the abdominal aortic PVAT (aPVAT). In addition, a high-fat diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the abdominal aorta. In contrast, a high-sucrose diet induced increased number of large-sized lipid droplets, increased CD68+ macrophage- and MCP-1-positive areas, and decreased UCP-1 positive area in the thoracic aortic PVAT (tPVAT). A high-sucrose diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the thoracic aorta. These results could be attributed to the different adipocyte populations in the tPVAT and aPVAT. Our results provide pathological evidence to improve our understanding of the relationship between diet and vascular diseases.
DOI: 10.24400/802406/rw7v-jbdz
title: MMS-2 SCM L2 Survey Quicklooks (6 hours) (PNG)

description: MMS-2 SCM survey quicklooks [0.0625-16Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the 6 hours) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/rn46-2kpq
title: MMS-2 SCM L2 Burst Quicklooks (PNG)

description: MMS-2 SCM burst quicklooks [1-4096Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the burst time period) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/eeqm-b0vw
title: MMS-2 SCM L2 High-Speed Burst Quicklooks (PNG)

description: MMS-2 SCM high-speed burst quicklooks [32-8192Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the burst time period) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.5281/zenodo.5112121
title: GPRChinaTemp1km: 1 km monthly maximum air temperature for China from January 1951 to December 2020

description: GPRChinaTemp1km is a new high-resolution (1-km) monthly gridded air temperature dataset for China from January 1951 to December 2020. The dataset includes monthly maximum air temperature covering the main land area of China during 1951-2020, which was interpolated by the Gaussian process regression (GPR) method based on the meteorological station data. The monthly gridded temperature dataset was evaluated by the observed values of the meteorological stations from the China Meteorological Data Service Centre. The dataset is in GeoTIFF format in the WGS84 (EPSG:4326) coordinate system. The unit of the data is degree Celsius (°C).
DOI: 10.24400/802406/k0ck-aex8
title: MMS-3 SCM L2 Survey Quicklooks (6 hours) (PNG)

description: MMS-3 SCM survey quicklooks [0.0625-16Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the 6 hours) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/79rt-f1ge
title: MMS-3 SCM L2 Burst Quicklooks (PNG)

description: MMS-3 SCM burst quicklooks [1-4096Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the burst time period) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/60an-x9h2
title: MMS-3 SCM L2 High-Speed Burst Quicklooks (PNG)

description: MMS-3 SCM high-speed burst quicklooks [32-8192Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the burst time period) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/wavc-g37e
title: MMS-4 SCM L2 Survey Quicklooks (6 hours) (PNG)

description: MMS-4 SCM survey quicklooks [0.0625-16Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the 6 hours) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/kads-zqx6
title: MMS-4 SCM L2 Burst Quicklooks (PNG)

description: MMS-4 SCM burst quicklooks [1-4096Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the burst time period) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.
DOI: 10.24400/802406/pcxd-m3jj
title: MMS-4 SCM L2 High-Speed Burst Quicklooks (PNG)

description: MMS-4 SCM high-speed burst quicklooks [32-8192Hz] shows from top to bottom : color coded data quality, waveforms (nT), power spectral densities (nT^2/Hz), integrated power (nT^2), time-averaged (over the burst time period) Fourier spectra (nT^2/Hz) with SCM ground noise level (in black) for reference. All data are plotted in GSE.