cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.57760/sciencedb.j00217.06800
title: Bioinformatics Analysis, Prokaryotic Expression, and Antiserum Preparation of hnRNP A1 Protein
title: hnRNP A1蛋白生物信息学分析、原核表达及抗血清制备

description: Objective This study aimed to optimize the expression conditions of hnRNP A1 protein in BL21 competent cells through methods such as PCR and double enzyme digestion, to obtain high-concentration and high-quality purified protein, and to prepare high-titer polyclonal antibodies against hnRNP A1.Methods Bioinformatics tools were used to analyze the physicochemical properties and structure of hnRNP A1. The pET-28a-hnRNP A1 recombinant plasmid was constructed and transformed into BL21 cells. After optimizing expression conditions, hnRNP A1 protein was purified using nickel column chromatography and identified by Western Blot. The purified protein was used to immunize C57BL mice to produce polyclonal antibodies, and the antibody titer was determined by indirect ELISA.Results The highest expression of hnRNP A1 was achieved under conditions of 0.4 mM IPTG, induction temperature of 42°C, and induction time of 8 hours. The purified protein concentration reached 2.0563 μg/μl, and Western Blot confirmed the target protein. The antibody titer detected by indirect ELISA was 1:409,600.Conclusion The physicochemical properties of hnRNP A1 were successfully analyzed, high-efficiency expression of hnRNP A1 protein was achieved, and high-titer mouse-derived polyclonal antibodies against hnRNP A1 were prepared, providing a valuable tool for further research.
description: Objective This study aimed to optimize the expression conditions of hnRNP A1 protein in BL21 competent cells through methods such as PCR and double enzyme digestion, to obtain high-concentration and high-quality purified protein, and to prepare high-titer polyclonal antibodies against hnRNP A1.Methods Bioinformatics tools were used to analyze the physicochemical properties and structure of hnRNP A1. The pET-28a-hnRNP A1 recombinant plasmid was constructed and transformed into BL21 cells. After optimizing expression conditions, hnRNP A1 protein was purified using nickel column chromatography and identified by Western Blot. The purified protein was used to immunize C57BL mice to produce polyclonal antibodies, and the antibody titer was determined by indirect ELISA.Results The highest expression of hnRNP A1 was achieved under conditions of 0.4 mM IPTG, induction temperature of 42°C, and induction time of 8 hours. The purified protein concentration reached 2.0563 μg/μl, and Western Blot confirmed the target protein. The antibody titer detected by indirect ELISA was 1:409,600.Conclusion The physicochemical properties of hnRNP A1 were successfully analyzed, high-efficiency expression of hnRNP A1 protein was achieved, and high-titer mouse-derived polyclonal antibodies against hnRNP A1 were prepared, providing a valuable tool for further research.
DOI: 10.57760/sciencedb.22769
title: VGLL1 cooperates with TEAD4 to control human trophectoderm lineage specification
title: VGLL1 cooperates with TEAD4 to control human trophectoderm lineage specification

description: Placenta abnormality is one of the key reasons for early pregnancy loss but the regulatory mechanisms underlying placenta formation are largely unknown. Here, we applied a human naïve pluripotent stem cell (PSC) derived TE model to explore the driven regulatory machinery. We demonstrate that VGLL1 (vestigial like family member 1) is crucial for TE self-renewal and TE-specific gene expression, thus suppressing VGLL1 leads to severely impaired cell proliferation and skewed TE induction. VGLL1 exerts its function through interacting with TEAD4 (TEA domain transcription factor 4) and colocalize at target gene promoters and enhancers. Further investigation uncovers the enrichment of H3K27ac active histone marks at genomic regions occupied by VGLL1-TEAD4 complex, indicates a close association between them. Overall, our data reveals the critical function model of VGLL1-TEAD4 in human TE derivation, highlighting a potential interspecies difference between human and mouse TE induction.
description: Placenta abnormality is one of the key reasons for early pregnancy loss but the regulatory mechanisms underlying placenta formation are largely unknown. Here, we applied a human naïve pluripotent stem cell (PSC) derived TE model to explore the driven regulatory machinery. We demonstrate that VGLL1 (vestigial like family member 1) is crucial for TE self-renewal and TE-specific gene expression, thus suppressing VGLL1 leads to severely impaired cell proliferation and skewed TE induction. VGLL1 exerts its function through interacting with TEAD4 (TEA domain transcription factor 4) and colocalize at target gene promoters and enhancers. Further investigation uncovers the enrichment of H3K27ac active histone marks at genomic regions occupied by VGLL1-TEAD4 complex, indicates a close association between them. Overall, our data reveals the critical function model of VGLL1-TEAD4 in human TE derivation, highlighting a potential interspecies difference between human and mouse TE induction.
DOI: 10.15468/dl.ujc7e3
title: Occurrence Download

description: A dataset containing 149723 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Trametes versicolor (L.) Lloyd"
 ]
}

 The dataset includes 149723 records from 614 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001258-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.57760/sciencedb.cryosphere.00006
title: A dataset of precipitation partitioning of Pinus massoniana and Castanopsis hystrix plantation in South Subtropical from 2018 to 2021
title: 2018–2021年南亚热带马尾松和红椎人工林降水分配数据集

description: 为深入研究南亚热带马尾松和杜鹃花人工林优势树种的降水分布格局特征，本研究依托广西友谊关森林生态系统国家定位观测研究站。2018 年 1 月至 2021 年 12 月，在中国林业科学研究院热带林业实验中心的林区，南亚热带优势树种马尾松 （Pinus massoniana Lamb）。和 Castanopsis hystrix Miq.以人工林为研究对象，对林外降雨量、森林流中、森林流中、树干和茎流进行连续动态监测和研究。小区布局和监测均按规定进行，有效保证了观测数据的质量和完整性。该数据集经过分析和处理，获得了红松和马森松林外降雨量、森林内水流以及树干和树干流的 24 小时测量数据。还解释了用于数据处理和质量控制的数据集的构建。建立和共享该数据集可以为深入研究森林水分利用模式及其对全球气候变化的响应提供基础数据，并为南亚热带森林生态系统的可持续经营和管理提供科学参考。
description: 为深入研究南亚热带马尾松和杜鹃花人工林优势树种的降水分布格局特征，本研究依托广西友谊关森林生态系统国家定位观测研究站。2018 年 1 月至 2021 年 12 月，在中国林业科学研究院热带林业实验中心的林区，南亚热带优势树种马尾松 （Pinus massoniana Lamb）。和 Castanopsis hystrix Miq.以人工林为研究对象，对林外降雨量、森林流中、森林流中、树干和茎流进行连续动态监测和研究。小区布局和监测均按规定进行，有效保证了观测数据的质量和完整性。该数据集经过分析和处理，获得了红松和马森松林外降雨量、森林内水流以及树干和树干流的 24 小时测量数据。还解释了用于数据处理和质量控制的数据集的构建。建立和共享该数据集可以为深入研究森林水分利用模式及其对全球气候变化的响应提供基础数据，并为南亚热带森林生态系统的可持续经营和管理提供科学参考。
DOI: 10.57760/sciencedb.cryosphere.00009
title: Soil species dataset of Xinjiang based on the Second National Soil Census
title: 基于第二次全国土壤普查的新疆土种志数据集

description: 该数据集根据1993年出版的新疆土壤物种汇编汇总，该数据集是新疆第二次全国土壤普查的结果之一，系统地总结和整理了344种土壤物种的归属和分布、主要性状、生产性能概况、典型剖面理化性质等更详细的信息。基于土壤科学专业的信息特点，为促进未来在线服务的发展，我们以信息丰富、标准化和结构化的方式收集和提取原始信息和数据，整合了以土壤物种 ID 和土壤物种名称为关键指标字段的五个学科数据表， 即土壤种类分类、土壤种类基本信息、典型剖面景观信息、典型剖面发生层调查信息、理化性质。为了更好地利用数据，数据表中还组织了两个时间表，即土壤物种分类指标表和土壤层次结构名称表。原始数据来源可靠，采集方式专业可行。这些数据可以充分服务于第三次全国土壤普查，可广泛应用于区域土地质量评价、生态环境恢复和土壤科学的研究，为土壤资源的合理利用提供极具意义的科学支撑。
description: 该数据集根据1993年出版的新疆土壤物种汇编汇总，该数据集是新疆第二次全国土壤普查的结果之一，系统地总结和整理了344种土壤物种的归属和分布、主要性状、生产性能概况、典型剖面理化性质等更详细的信息。基于土壤科学专业的信息特点，为促进未来在线服务的发展，我们以信息丰富、标准化和结构化的方式收集和提取原始信息和数据，整合了以土壤物种 ID 和土壤物种名称为关键指标字段的五个学科数据表， 即土壤种类分类、土壤种类基本信息、典型剖面景观信息、典型剖面发生层调查信息、理化性质。为了更好地利用数据，数据表中还组织了两个时间表，即土壤物种分类指标表和土壤层次结构名称表。原始数据来源可靠，采集方式专业可行。这些数据可以充分服务于第三次全国土壤普查，可广泛应用于区域土地质量评价、生态环境恢复和土壤科学的研究，为土壤资源的合理利用提供极具意义的科学支撑。
DOI: 10.57760/sciencedb.j00206.00066
title: supplementary materials
title: 补充材料

description: Proof of Estimation of Partial Functional Linear Quantile Regression Model with Variable Coefficient under Missing Data in Manuscripts 
description: Proof of Estimation of Partial Functional Linear Quantile Regression Model with Variable Coefficient under Missing Data in Manuscripts
DOI: 10.57760/sciencedb.22789
title: The date of Efficient high power Yb:LuAG Innoslab amplifier compressible to 360 fs
title: The date of Efficient high power Yb:LuAG Innoslab amplifier compressible to 360 fs

description: This data is sourced from the recorded data in the experiment
description: This data is sourced from the recorded data in the experiment
DOI: 10.57760/sciencedb.cast.00018
title: the ecological environment quality of ecological land-covers in the low-mountainous and hilly area of central Shandong Province
title: 2013-2023鲁中低山区生态质量状况评级年值

description: In this study, a constructed remote sensing-based ecological index (RSEI), which was based on the monthly dataset from the growing seasons (2013-2023), was used to assess forest-dominated land-cover types, Secondly, and statistically analyzed the spatiotemporal variation characteristics of RSEI and its ecological response mechanisms by integrating key ecological factors, including biomass and vegetation coverage, Finally, and completed the spatial gradient analysis of both RSEI and biomass based on different levels of the digital elevation models (DEM).
description: In this study, a constructed remote sensing-based ecological index (RSEI), which was based on the monthly dataset from the growing seasons (2013-2023), was used to assess forest-dominated land-cover types, Secondly, and statistically analyzed the spatiotemporal variation characteristics of RSEI and its ecological response mechanisms by integrating key ecological factors, including biomass and vegetation coverage, Finally, and completed the spatial gradient analysis of both RSEI and biomass based on different levels of the digital elevation models (DEM).
DOI: 10.57760/sciencedb.22803
title: Metallic calcium ion observational data; Ionosonde observational data; Geomagnetic data
title: Metallic calcium ion observational data; Ionosonde observational data; Geomagnetic data

description: The resolution of the metallic ion dataset is 1 minute and 96 meters.The ionosonde data have a resolution of 15 minutes and are manually calibrated using the SAO software.
description: The resolution of the metallic ion dataset is 1 minute and 96 meters.The ionosonde data have a resolution of 15 minutes and are manually calibrated using the SAO software.
DOI: 10.57760/sciencedb.22804
title: Bacterial infection in ACLF patients
title: Bacterial infection in ACLF patients

description: Bacterial infection is an important risk factor for the occurrence and development of chronic acute liver failure (ACLF). Bacterial infection significantly exacerbates the severity and clinical course of ACLF, resulting in a significantly higher mortality rate in ACLF patients with bacterial infection compared to those without bacterial infection. Here, we recorded baseline data, survival status, microbiological data, and antibiotic use of ACLF infected patients. 
description: Bacterial infection is an important risk factor for the occurrence and development of chronic acute liver failure (ACLF). Bacterial infection significantly exacerbates the severity and clinical course of ACLF, resulting in a significantly higher mortality rate in ACLF patients with bacterial infection compared to those without bacterial infection. Here, we recorded baseline data, survival status, microbiological data, and antibiotic use of ACLF infected patients.
DOI: 10.15468/dl.xtm6rg
title: Occurrence Download

description: A dataset listing the 5091 species recorded in GBIF matching the query:
{
 "DatasetKey" : [
 "is Fungal atlas"
 ]
}

 The dataset's 5091 records were derived from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001267-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.57760/sciencedb.22809
title: Supporting Data of "Web of Common Prosperity: How Peer Effects Shaped Rural Households' Risk Assets Investment Behavior in China"
title: Supporting Data of "Web of Common Prosperity: How Peer Effects Shaped Rural Households' Risk Assets Investment Behavior in China"

description: Supporting Data of "Web of Common Prosperity: How Peer Effects Shaped Rural Households' Risk Assets Investment Behavior in China"
description: Supporting Data of "Web of Common Prosperity: How Peer Effects Shaped Rural Households' Risk Assets Investment Behavior in China"
DOI: 10.57760/sciencedb.22828
title: Monthly multi-year average snow density grid dataset on the Tibetan Plateau
title: 青藏高原逐月多年平均积雪密度格点数据集

description: 雪密度是表征积雪的重要参数，是将雪深转换为雪水当量的关键指标。它在山区雪水资源估算、融雪洪水等水资源管理、自然灾害预报和气候研究方面也发挥着至关重要的作用。以 1960—2020 年青藏高原 132 个日日国家气象站、中国区域地表气象要素驱动数据集和卫星融合雪深数据集为主要数据源，比较了几种机器学习模型在不同地表类型模拟雪密度方面的性能，并选择了最优模型。整合地面、卫星和再分析数据，我们最终制作了青藏高原多年平均雪密度网格的数据集。使用青藏高原 132 个国家气象站的逐月多年平均雪密度数据进行准确性评估，平均均方根误差为 0.019 g/cm3，平均相对误差为 11.88%，表明数据集的准确性很高。该数据集有望为青藏高原水资源评价和水文过程模拟提供数据支持。
description: 雪密度是表征积雪的重要参数，是将雪深转换为雪水当量的关键指标。它在山区雪水资源估算、融雪洪水等水资源管理、自然灾害预报和气候研究方面也发挥着至关重要的作用。以 1960—2020 年青藏高原 132 个日日国家气象站、中国区域地表气象要素驱动数据集和卫星融合雪深数据集为主要数据源，比较了几种机器学习模型在不同地表类型模拟雪密度方面的性能，并选择了最优模型。整合地面、卫星和再分析数据，我们最终制作了青藏高原多年平均雪密度网格的数据集。使用青藏高原 132 个国家气象站的逐月多年平均雪密度数据进行准确性评估，平均均方根误差为 0.019 g/cm3，平均相对误差为 11.88%，表明数据集的准确性很高。该数据集有望为青藏高原水资源评价和水文过程模拟提供数据支持。
DOI: 10.57760/sciencedb.22801
title: DATA used in paper "Enhancement of near-inertial waves by cyclonic eddy in the northwestern South China Sea during spring 2022"
title: DATA used in paper "Enhancement of near-inertial waves by cyclonic eddy in the northwestern South China Sea during spring 2022"

description: Data and code using in "Enhancement of near-inertial waves by cyclonic eddy in the northwestern South China Sea during spring 2022" submitted to Ocean SciencePlease let me know if you need any more information.
description: Data and code using in "Enhancement of near-inertial waves by cyclonic eddy in the northwestern South China Sea during spring 2022" submitted to Ocean SciencePlease let me know if you need any more information.
DOI: 10.15468/dl.dnxdz5
title: Occurrence Download

description: A dataset containing 516723 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Furnarius rufus (Gmelin, 1788)"
 ]
}

 The dataset includes 516723 records from 134 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001257-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.jubb6p
title: Occurrence Download

description: A dataset containing 68 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is United States of America",
 "DatasetKey is iNaturalist Research-grade Observations",
 "StateProvince is NOrth Carolina",
 "TaxonKey is Chelonia mydas (Linnaeus, 1758)"
 ]
}

 The dataset includes 68 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001268-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.57760/sciencedb.22836
title: Raw data
title: Raw data

description: This is the FFA and PC data exported from imotions software, and the SPSS file used for statistics
description: This is the FFA and PC data exported from imotions software, and the SPSS file used for statistics
DOI: 10.57760/sciencedb.j00113.00218
title: Spectroscopic and transition properties of strontium chloride
title: Spectroscopic and transition properties of strontium chloride

description: The spectroscopic and transition properties of strontium chloride (SrCl) is investigated based on a theoretical approach of ab initio quantum chemistry. The scalar relativistic and spin-orbit coupling (SOC) effect is considered in calculation by introducing Davidson correction, core-valence correlation (CV), The calculation accuracy is improved The results show that the spectroscopic constants of X2Σ+ and A2Π states are consistent with the experimental results. The spectroscopic and molecular constants of most highly excited electronic states have been reported for the first time. The permanent dipole moment (PDMs) and the spin orbit (SO) matrix element have a sudden change for the avoidance of crossing. The potential energy curves (PECs) of the 14 Λ-S states split into 30 Ω states. The splitting energy of A2Π is 290.76 cm-1, which has little difference from the experimental value 295.597 cm-1. Finally, the transition properties are given, including transition dipole moment (TDMs), Franck-Canton factor (FCFs) and radiation lifetime. It is found that the calculated radiation lifetime is in the order of 10ns. The research provides theoretical reference for the feasibility laser cooling of SrCl molecule. The dataset that supported the findings of this study is available in Science Data Bank, including the permanent dipole moment, the spin orbit matrix element and the transition dipole moment .
description: The spectroscopic and transition properties of strontium chloride (SrCl) is investigated based on a theoretical approach of ab initio quantum chemistry. The scalar relativistic and spin-orbit coupling (SOC) effect is considered in calculation by introducing Davidson correction, core-valence correlation (CV), The calculation accuracy is improved The results show that the spectroscopic constants of X2Σ+ and A2Π states are consistent with the experimental results. The spectroscopic and molecular constants of most highly excited electronic states have been reported for the first time. The permanent dipole moment (PDMs) and the spin orbit (SO) matrix element have a sudden change for the avoidance of crossing. The potential energy curves (PECs) of the 14 Λ-S states split into 30 Ω states. The splitting energy of A2Π is 290.76 cm-1, which has little difference from the experimental value 295.597 cm-1. Finally, the transition properties are given, including transition dipole moment (TDMs), Franck-Canton factor (FCFs) and radiation lifetime. It is found that the calculated radiation lifetime is in the order of 10ns. The research provides theoretical reference for the feasibility laser cooling of SrCl molecule. The dataset that supported the findings of this study is available in Science Data Bank, including the permanent dipole moment, the spin orbit matrix element and the transition dipole moment .
DOI: 10.15468/dl.m2yh8d
title: Occurrence Download

description: A dataset containing 4185 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is North America",
 "Month is April",
 "OccurrenceStatus is Present",
 "TaxonKey is Zonotrichia leucophrys (J.R.Forster, 1772)",
 "Year 2006-2006"
 ]
}

 The dataset includes 4185 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001256-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.57760/sciencedb.j00100.00017
title: The dataset on the relationship between the built environment and the metro ridership of mobility-vulnerable groups
title: 建成环境与出行脆弱群体的地铁出行关联机制研究数据集

description: The dataset consists of three parts:Hyperparameter design and goodness-of-fit results of random forest regression model, GBDT regression model, XGBoost review model and OLS regression model.OLS regression model results for comparison with GBDT review model results.Partial dependency plots between metro ridership and built environment attributes for the senior, students and general population.
description: The dataset consists of three parts:Hyperparameter design and goodness-of-fit results of random forest regression model, GBDT regression model, XGBoost review model and OLS regression model.OLS regression model results for comparison with GBDT review model results.Partial dependency plots between metro ridership and built environment attributes for the senior, students and general population.
DOI: 10.57760/sciencedb.j00217.06865
title: Mechanism of Proanthocyanidins B2 in Alleviating Experimental Autoimmune Encephalomyelitis via Inhibition of Th17 Cells
title: 原花青素B2抑制Th17缓解实验性变态反应性脑脊髓炎的机制研究

description: Objective Proanthocyanidins B2 (PC-B2), a naturally derived antioxidant, exhibits potent anti-inflammatory properties. Multiple sclerosis (MS), an autoimmune demyelinating disease of the central nervous system (CNS), is driven by neuroinflammation. This study aimed to investigate the therapeutic potential and mechanisms of PC-B2 in experimental autoimmune encephalomyelitis (EAE), a murine model of MS.Methods EAE-induced mice received intraperitoneal injections of PC-B2 (50 mg/kg) from day 10 (post-symptom onset) until day 18, followed by sacrifice. Luxol fast blue staining, immunofluorescence, flow cytometry, ELISA, and Western blotting were employed to assess PC-B2’s effects on demyelination, inflammatory responses, and T-cell dynamics in the CNS and peripheral spleen.Results PC-B2 significantly alleviated spinal cord demyelination and reduced the secretion of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12, and IL-23) in both the CNS and spleen. Furthermore, PC-B2 suppressed Th17 cell polarization and downregulated the expression of interferon regulatory factor 4 (IRF4) in Th17 cells.Conclusion These findings demonstrate that PC-B2 exerts therapeutic effects in EAE by inhibiting IRF4-mediated Th17 polarization, thereby reshaping the inflammatory microenvironment. This highlights PC-B2 as a promising candidate for MS treatment.
description: Objective Proanthocyanidins B2 (PC-B2), a naturally derived antioxidant, exhibits potent anti-inflammatory properties. Multiple sclerosis (MS), an autoimmune demyelinating disease of the central nervous system (CNS), is driven by neuroinflammation. This study aimed to investigate the therapeutic potential and mechanisms of PC-B2 in experimental autoimmune encephalomyelitis (EAE), a murine model of MS.Methods EAE-induced mice received intraperitoneal injections of PC-B2 (50 mg/kg) from day 10 (post-symptom onset) until day 18, followed by sacrifice. Luxol fast blue staining, immunofluorescence, flow cytometry, ELISA, and Western blotting were employed to assess PC-B2’s effects on demyelination, inflammatory responses, and T-cell dynamics in the CNS and peripheral spleen.Results PC-B2 significantly alleviated spinal cord demyelination and reduced the secretion of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12, and IL-23) in both the CNS and spleen. Furthermore, PC-B2 suppressed Th17 cell polarization and downregulated the expression of interferon regulatory factor 4 (IRF4) in Th17 cells.Conclusion These findings demonstrate that PC-B2 exerts therapeutic effects in EAE by inhibiting IRF4-mediated Th17 polarization, thereby reshaping the inflammatory microenvironment. This highlights PC-B2 as a promising candidate for MS treatment.