DOI: 10.5061/dryad.c866t1gkj
title: Data for: Ontogenetic and geographic phytochemical variation in <em>Mimulus moschatus</em>, a perennial monkeyflower

description: Secondary metabolites are critical to plant defenses and frequently
 exhibit variation among populations associated with heterogeneous climatic
 factors and/or herbivore communities. We examined genetic-based geographic
 and ontogenetic variation in phenylpropanoid glycoside (PPG)
 concentrations and arsenals in Mimulus moschatus, a perennial monkeyflower
 native to western North America. We conducted a greenhouse common garden
 with maternal lines collected from three populations sampled along a
 latitudinal gradient paralleling spatial sampling from a previous study in
 the closely related congener, Mimulus guttatus. We assessed PPG arsenals
 as well as concentrations of individual and total PPGs within leaf tissue
 from two developmental stages. Populations differed in total concentration
 and arsenal composition of PPGs, with the populations from intermediate
 latitudes exhibiting higher concentrations than higher and lower latitude
 populations. Leaves from earlier developmental stages had higher overall
 PPG concentrations than leaves from later developmental stages across all
 populations, suggesting juvenile tissue is better defended than more
 mature tissue. Spatial patterns in relative PPG concentrations and arsenal
 composition tightly paralleled those in the closely related M. guttatus
 across space despite consistently lower total PPG concentrations in M.
 moschatus. Because we did not measure herbivory and our sample size is
 limited, these results should be interpreted cautiously. Nonetheless, they
 provide baseline data for understanding geographic and developmental
 variation in chemical defenses and generate hypotheses about environmental
 influences and potential convergence in defenses among closely related
 species.
description: # DATA AND FILE OVERVIEW FOR ONTOGENETIC AND GEOGRAPHIC PHYTOCHEMICAL
 VARIATION IN *MIMULUS MOSCHATUS*, A PERENNIAL MONKEYFLOWER This
 README_OGPV.txt file was generated on 2025-06-28 by Simon Innes. ###
 GENERAL INFORMATION 1. Title of Dataset: “Data for: Ontogenetic and
 geographic phytochemical variation in *Mimulus moschatus*, a perennial
 monkeyflower.” 2. Author Information: \   Corresponding author \   Name:
 Simon Innes \   Institution: University of Louisiana at Lafayette \  
 Email: [simon.innesz@gmail.com](mailto:simon.innesz@gmail.com)    
 Co-author \      Name: Liza Holeski \      Institution: Northern Arizona
 University     Senior author \      Name: Nicholas Kooyers \     
 Institution: University of Louisiana at Lafayette 1. Date of data
 collection: 2021 2. Geographic location of data collection: see
 musky_monk_env.csv & Kooyers_et_al_2017_simplified.csv 3. Unit of
 measure for concentrations: Individual and total PPG concentrations are
 calculated as milligrams of compound per gram of dry leaf tissue.  4.
 Funding: This work was funded by a National Science Foundation CAREER
 grant (DEB-2045643) awarded to NJK. ### DATA AND FILE OVERVIEW 1. File
 list: \   File 1: "Musky_PPG_conc.csv" \   Description: Raw data
 of total and individual PPG concentrations for each *Mimulus moschatus*
 sample.     File 2: "musky_monk_env.csv" \      Description:
 Geographic locations and metadata for sampled *Mimulus moschatus*
 populations.     File 3: "mos_gutt_PPG_pops.csv" \     
 Description: Geographic locations of *Mimulus guttatus* populations from
 Kooyer et al. (2017) and *M. moschatus* populations from present study.
     File 4: "Kooyers_et_al_2017_simplified.csv" \     
 Description: Simplified dataset of raw total and individual PPG
 concentrations of *Mimulus guttatus* samples from populations included in
 this study.     File 5: "PPG_mean_SD_TableS1.csv" \     
 Description: Summary of means and standard deviations of *Mimulus
 moschatus* PPGs across populations and ontogeny.     File 6:
 "PPG_cor_TableS2.csv" \      Description: Summary of
 correlations and associated *p*-values among individual and total PPGs of
 *Mimulus moschatus*.     File 7: "PPG_NMDS_var_TableS3.csv" \
      Description: Summary of explanatory power of individual *Mimulus
 moschatus* PPGs on non-metric multidimensional scaling (NMDS) dimensions.
 #### DATA SPECIFIC INFORMATION FOR: "Musky_PPG_conc.csv" 1.
 Number of variables: 15 2. Number of cases/rows: 48 3. Variable List: \  
 Sample Name: Sample specific identification number \   PPG b:
 Concentration of PPG b \   PPG c: Concentration of PPG c \   PPG d:
 Concentration of PPG d \   PPG e: Concentration of PPG e \   PPG f:
 Concentration of PPG f \   PPG g: Concentration of PPG g \   PPG h:
 Concentration of PPG h \   Calc A: Concentration of calceolarioside A \  
 Conand: Concentration of conandroside \   Verb: Concentration of
 verbascoside \   Calc B: Concentration of calceolarioside B \   Mimulo:
 Concentration of mimuloside \   Unkn 16: Concentration of unknown PPG 16 \
   Total PPGs: Concentration of total PPGs 4. Missing data codes: \   none
 5. Specialized formats or other abbreviations used: \   PPG:
 Phenylpropanoid glycoside \   Calc A: Calceolarioside A \   Conand:
 Conandroside \   Verb: Verbascoside \   Calc B: Calceolarioside B \  
 Mimulo: Mimuloside \   Unkn 16: Unkown phenylpropanoid glycoside 16 \  
 PPGs: Phenylpropanoid glycosides #### DATA SPECIFIC INFORMATION FOR:
 "musky_monk_env.csv" 1. Number of variables: 6 2. Number of
 cases/rows: 3 3. Variable List: \   pop_name: Unique population name \  
 pop_num: Unique population number \   latitude: Latitudinal coordinates of
 population in decimal degrees \   longitude: Longitudinal coordinates of
 population in decimal degrees \   elevation_m: Elevation of population in
 meters \   range: Categorical variable describing the region where a
 population was sampled (three levels) 4. Missing data codes: \   none 5.
 Specialized formats or other abbreviations used: \   PPG: Phenylpropanoid
 glycoside \   pop: Population \   num: Number \   m: Meter #### DATA
 SPECIFIC INFORMATION FOR: "mos_gutt_PPG_pops.csv" 1. Number of
 variables: 5 2. Number of cases/rows: 38 3. Variable List: \   pop_name:
 Unique population name \   latitude: Latitudinal coordinates of population
 in decimal degrees \   longitude: Longitudinal coordinates of population
 in decimal degrees \   elevation_m: Elevation of population in meters \  
 species: Categorical variable descirbing the species identity (mos vs.
 gutt; two levels) 4. Missing data codes: \   none 5. Specialized formats
 or other abbreviations used: \   pop: Population \   m: Meter \   mos:
 *Mimulus moschatus* \   gutt: *Mimulus guttatus* #### DATA SPECIFIC
 INFORMATION FOR: "Kooyers_et_al_2017_simplified.csv" 1. Number
 of variables: 16 2. Number of cases/rows: 23 3. Variable List: \  
 pop_name: Unique population name \   ind: Unique indentifer corresponding
 to a maternal line within a population \   rep: Unique identifier
 corresponding to a replicate within a maternal line \   Unkn 10:
 Concentration of unknown PPG 10 \   Calc A: Concentration of
 calceolarioside A \   Conand: Concentration of conandroside \   Verb:
 Concentration of verbascoside \   Calc B: Concentration of calceolarioside
 B \   Mimulo: Concentration of mimuloside \   Unkn 16: Concentration of
 unknown PPG 16 \   Total PPGs: Concentration of total PPGs \   latitude:
 Latitudinal coordinates of population in decimal degrees \   longitude:
 Longitudinal coordinates of population in decimal degrees \   elevation_m:
 Elevation in meters \   species: Categorical variable descirbing the
 species identity (gutt; one level) \   range: Categorical variable
 describing the region where a population was sampled (three levels) 4.
 Missing data codes: \   none 5. Specialized formats or other abbreviations
 used: \   pop: Population \   ind: Individual \   rep: Replicate \   Calc
 A: Calceolarioside A \   Conand: Conandroside \   Verb: Verbascoside \  
 Calc B: Calceolarioside B \   Mimulo: Mimuloside \   Unkn 16: Unkown
 phenylpropanoid glycoside 16 \   PPGs: Phenylpropanoid glycosides \   m:
 Meter \   gutt: *Mimulus guttatus* #### DATA SPECIFIC INFORMATION FOR:
 "PPG_mean_SD_TableS1.csv" 1. Number of variables: 30 2. Number
 of cases/rows: 6 3. Variable List: \   Population: Unique population name
 \   Ontogeny: Developmental stage of leaf sample (early vs. late; two
 levels) \   PPG b_mean: Mean concentration of PPG b \   PPG b_sd: Standard
 deviation of PPG b concentration \   PPG c_mean: Mean concentration of PPG
 c \   PPG c_sd: Standard deviation of PPG c concentration \   PPG d_mean:
 Mean concentration of PPG d \   PPG d_sd: Standard deviation of PPG d
 concentration \   PPG e_mean: Mean concentration of PPG e \   PPG e_sd:
 Standard deviation of PPG e concentration \   PPG f_mean: Mean
 concentration of PPG f \   PPG f_sd: Standard deviation of PPG f
 concentration \   PPG g_mean: Mean concentration of PPG g \   PPG g_sd:
 Standard deviation of PPG g concentration \   PPG h_mean: Mean
 concentration of PPG h \   PPG h_sd: Standard deviation of PPG h
 concentration \   Calc A_mean: Mean concentration of calceolarioside A \  
 Calc A_sd: Standard deviation of calceolarioside A concentration \  
 Conand_mean: Mean concentration of conandroside \   Conand_sd: Standard
 deviation of conandroside concentration \   Verb_mean: Mean concentration
 of verbascoside \   Verb_sd: Standard deviation of verbascoside
 concentration \   Calc B_mean: Mean concentration of calceolarioside B \  
 Calc B_sd: Standard deviation of calceolarioside B concentration \  
 Mimulo_mean: Mean concentration of mimuloside \   Mimulo_sd: Standard
 deviation of mimuloside concentration \   Unkn 16_mean: Mean concentration
 of unknown PPG 16 \   Unkn 16_sd: Standard deviation of unknown PPG 16
 concentration \   Total PPGs_mean: Mean concentration of total PPGs \  
 Total PPGs_sd: Standard deviation of total PPGs concentration 4. Missing
 data codes: \   none 5. Specialized formats or other abbreviations used: \
   PPG: Phenylpropanoid glycoside \   Calc A: Calceolarioside A \   Conand:
 Conandroside \   Verb: Verbascoside \   Calc B: Calceolarioside B \  
 Mimulo: Mimuloside \   Unkn 16: Unkown phenylpropanoid glycoside 16 \  
 PPGs: Phenylpropanoid glycosides \   sd: Standard deviation #### DATA
 SPECIFIC INFORMATION FOR: "PPG_cor_TableS2.csv" 1. Number of
 variables: 15 2. Number of cases/rows: 14 3. Variable List: \   PPG: Name
 of individual PPGs or total PPGs \   Total PPGs: Correlations and
 *p*-values of total PPGs with individual and total PPGs \   Calc A:
 Correlations and *p*-values of calceolarioside A with individual and total
 PPGs \   Conand: Correlations and *p*-values of conandroside with
 individual and total PPGs \   Verb: Correlations and *p*-values of
 verbascoside with individual and total PPGs \   Calc B: Correlations and
 *p*-values of verbascoside with individual and total PPGs \   Mimulo:
 Correlations and *p*-values of mimuloside with individual and total PPGs \
   Unkn 16: Correlations and *p*-values of unknown PPG 16 with individual
 and total PPGs \   PPG b: Correlations and *p*-values of PPG b with
 individual and total PPGs \   PPG c: Correlations and *p*-values of PPG c
 with individual and total PPGs \   PPG d: Correlations and *p*-values of
 PPG d with individual and total PPGs \   PPG e: Correlations and
 *p*-values of PPG e with individual and total PPGs \   PPG f: Correlations
 and *p*-values of PPG f with individual and total PPGs \   PPG g:
 Correlations and *p*-values of PPG g with individual and total PPGs \  
 PPG h: Correlations and *p*-values of PPG h with individual and total PPGs
 4. Missing data codes: \   none 5. Specialized formats or other
 abbreviations used: \   PPG: Phenylpropanoid glycoside \   Calc A:
 Calceolarioside A \   Conand: Conandroside \   Verb: Verbascoside \   Calc
 B: Calceolarioside B \   Mimulo: Mimuloside \   Unkn 16: Unkown
 phenylpropanoid glycoside 16 \   PPGs: Phenylpropanoid glycosides ####
 DATA SPECIFIC INFORMATION FOR: "PPG_NMDS_var_TableS3.csv" 1.
 Number of variables: 5 2. Number of cases/rows: 13 3. Variable List: \  
 PPG: Name of individual PPGs \   NMDS1: Correlation coefficients of NMDS1
 axis for each PPG \   NMDS2: Correlation coefficients of NMDS2 axis for
 each PPG \   *R*^2^: Strength of association between individual PPGs and
 the NMDS ordination \   *P*: Significance of association between
 individual PPGs and the NMDS ordination 4. Missing data codes: \   none 5.
 Specialized formats or other abbreviations used: \   NMDS: Non-metric
 multidimensional sacaling \   PPG: Phenylpropanoid glycoside \   Calc A:
 Calceolarioside A \   Conand: Conandroside \   Verb: Verbascoside \   Calc
 B: Calceolarioside B \   Mimulo: Mimuloside \   Unkn 16: Unkown
 phenylpropanoid glycoside 16 \   PPGs: Phenylpropanoid glycosides