DOI: 10.5061/dryad.gmsbcc2w6
title: Data from: Plant ammonium sensitivity is associated with the external pH adaptation, repertoire of nitrogen transporters, and nitrogen requirement

description: Modern crops exhibit diverse sensitivities to ammonium as the primary
 nitrogen source, influenced by environmental factors such as external pH
 and nutrient availability. Despite its significance, there is currently no
 systematic classification of plant species based on their ammonium
 sensitivity. This study conducts a meta-analysis of 50 plant species and
 presents a new classification method based on the comparison of fresh
 biomass obtained under ammonium and nitrate nutrition. The classification
 uses the natural logarithm of biomass ratio as the size effect indicator
 of ammonium sensitivity. This numerical parameter is associated with
 critical factors for nitrogen demand and form preference, such as
 Ellenberg indicators and the repertoire of nitrogen transporters for
 ammonium and nitrate uptake. Finally, a comparative analysis of the
 developmental and metabolic responses, including hormonal balance, is
 conducted in two species with divergent ammonium sensitivity values in the
 classification. Results indicate that nitrate has a key counteracting role
 of ammonium toxicity in species with a higher abundance of genes encoding
 NRT2-type proteins and fewer of the AMT2-type proteins. Additionally, the
 study confirms the reliability of the phytohormone balance and
 methylglyoxal content as indicators for anticipating ammonium toxicity.
description: # **Rivero-Marcos et al\_(2024\_JXB)\_RAW DATA**
 [https://doi.org/10.5061/dryad.gmsbcc2w6]() The Excel data file comprises
 the raw data for querying, estimating ammonium sensitivity, and
 replicating the analyses of the following paper: Rivero-Marcos M, Lasa B,
 Neves T, Zamarreño A.M, García-Mina J.M, García-Olaverri C, Aparicio-Tejo
 P.M, Cruz C, Ariz A. Plant ammonium sensitivity is associated with the
 external pH adaptation, repertoire of nitrogen transporters, and nitrogen
 requirement. ## Description of the data and file structure Each sheet in
 the Excel file contains the raw data corresponding to the figures in the
 paper, with methodology described in the Materials & Methods section.
 In brief: * Metaanalysis: Fresh weight biomass of shoot, root, and whole
 plant of species and cultivars grown hydroponically under certain
 concentrations of nitrate and ammonium, and pH, according to the studies
 in the metaanalysis, from which LnBR* and its variance were
 calculated.(*LnBR is defined as the natural logarithm of the ratio between
 the fresh biomass gained via ammonium or nitrate nutrition. Detail in the
 M&M section of the manuscript). * Forest plot_plant_sps: LnBR for
 plant species under hydroponic conditions for high (≥6.5) and lower
 (&lt;6.5) external pH. Distinction was made between species included or
 not in the metaanalysis (based on criteria in table 1 of the main text).
 Upper and lower limits are shown. * Forest plot_root_sps: Similar to the
 previous sheet, but referring to root tissue. * Forest plot_shoot_sps:
 Similar to the previous sheet, but referring to shoot tissue. * Forest
 plot_plant_cv: Similar to the previous sheet, but here is shown the
 response to ammonium sensitivity among different cultivars within the same
 species. * EIVs: Ellenberg Index Values for each species with information
 on *N* (nitrophily), *R* (pH), and *Spread* according to
 'Italian' and 'British' flora references,
 'GBIF' (Global Biodiversity Information Facility), and
 'PFAF' (Plant for a Future) databases. The number of members of
 the *NRT1*, *NRT2*, *AMT1*, and *AMT2*-onwards gene families per species
 was shown according to the linked references, or in the 'TAIR',
 'Spinach', and 'Pisum Cameor' databases. * So vs
 Ps_DW: Fresh weight biomass of root and leaf of two spinach (Winter-Giant)
 and pea (Sugar-Snap) cultivars according to the nitrogen and external pH
 regime in hydroponics. * So_pH 6_ions; Ps_pH 6_ions; So_pH 8_ions; Ps_pH
 8_ions: Raw contents of cations and anions in spinach (So) or pea (Ps)
 cultivar in root and shoot at external pH 6 and 8 according to the
 nitrogen regime. * So vs Ps_Hormones: Hormone concentration in spinach and
 pea cultivars as described in the Excel sheet, for shoot and root under pH
 6 and 8 according to the nitrogen regime (N='nitrate';
 A='ammonium'). * Phyl_Tree_NRT1s: Amino acid sequence used to
 construct the phylogenetic tree for identified members of the NRT1/NPF
 family in Arabidopsis and spinach (species more sensitive to ammonium),
 and pea and rice (more tolerant). * Phyl_Tree_NRT2s: Similar to the
 previous point for members of the NRT2 family. * Phyl_Tree_AMTs: Similar
 for the ammonium transporters of the AMT1 and AMT2-onwards families. In
 any case, empty cells or cells with the value N/A (not applicable)
 indicate that no data is available for the specific value in the cited
 paper (as seen in the meta-analysis sheet).