cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.26008/1912/bco-dmo.958329.1
title: Herbivorus green sea urchin counts from surveys conducted in St. Joseph Bay, Florida, USA in May 2016 and August 2019

description: The herbivorus green sea urchin (Lytechinus variegatus) was surveyed across the southern part of St. Joseph Bay at 16 sites spaced approximately 1 nautical mile apart. Initial surveys were performed in May 2016 as part of a prior study of seagrass coverage, reproduction, and herbivore intensity. Using RAPID funds, the same survey was conducted in August 2019, 11 months after Hurricane Michael, an extremely powerful category 5 tropical cyclone, made landfall nearby with devastating effects. Densities were recorded by snorkelers in 5 replicate, haphazardly thrown 0.25-m2 quadrats at each site. 

Comparing pre- and post-hurricane urchin densities allows for estimates of how Hurricane Michael's passage impacted resident urchin densities. These data were collected by Dorothy Byron and Alexandra Rodriguez of the Dauphin Island Sea Lab.
DOI: 10.26008/1912/bco-dmo.969869.1
title: HPLC estimates of phytoplankton pigments from cruise RR2201 on R/V Roger Revelle from Jan to Feb 2022 in the Argo Basin region off NW Australia

description: This dataset contains HPLC estimates of phytoplankton pigments from cruise RR2201 on R/V Roger Revelle (BLOOFINZ-IO, January-February 2022) in the Argo Basin region off NW Australia.
DOI: 10.26008/1912/bco-dmo.964151.1
title: Differential effects of nutrients and consumer pressure on sympatric cryptic coral species (Pocillopora spp.) in Moorea, French Polynesia sampled in November 2021.

description: Cryptic species (evolutionarily distinct lineages that do not align with morphologically defined species) are being increasingly discovered but are poorly integrated into ecological theory. In particular, we still lack a useful understanding of if and how cryptic species differ in ways that affect community recovery from disturbances and responses to anthropogenic stressors, such as the removal of consumers and pollution from nutrients. 

On coral reefs, nutrient pollution increases the growth of macroalgae that displaces corals. Reductions in herbivorous fishes reduce the suppression of macroalgae, while reductions in coralivorous fishes reduce predation on corals. An unresolved question is if and how cryptic coral species respond differently to these impacts, thereby differing in their ability to influence coral community dynamics and maintain coral dominance. Therefore, we assessed how the response of cryptic Pocillopora species over a period of three years following a simulated disturbance from a cyclone depended on the experimental reduction of fish consumer pressure and nutrient addition. After three years, five morphologically cryptic, but genetically distinct, Pocillopora species recruited to the reef. However, recruitment was dominated by two species: P. tuahiniensis (46%) and P. meandrina (43%). 

Under ambient conditions, recruitment of P. tuahiniensis and P. meandrina was similar, but experimentally reducing consumer pressure increased recruitment of P. tuahiniensis by up to 73% and reduced recruitment of P. meandrina by up to 49%. In both species, nutrient enrichment increased recruitment and colony growth rates equally, but colonies of P. tuahiniensis grew faster, and were up to 25% larger after three years, than those of P. meandrina, and growth was unaffected by reduced consumer pressure. Predation by excavating corallivorous fish was higher for P. meandrina than for P. tuahiniensis, especially under nutrient enrichment. In contrast, polyp extension (an indicator of elevated heterotrophic feeding as well as susceptibility and attractiveness to corallivores) was lower for P. meandrina than for P. tuahiniensis, especially under low to medium consumer pressure. Overall, we uncovered ecological differences in the response of morphologically cryptic foundation species to two pervasive stressors on coral reefs. 

Our results demonstrate how cryptic species respond differently to key anthropogenic stressors, which may contribute to response diversity that can support ecological resilience or increase extinction risk.
DOI: 10.26008/1912/bco-dmo.969309.1
title: Global dissolved thorium and protactinium database

description: We compiled a compilation of dissolved thorium (230Th and 232Th) and protactinium (231Pa) data measured by mass spectrometry, spanning from times preceding the GEOTRACES program until 2024. The dataset is available for model validation and data-model comparisons.
DOI: 10.26008/1912/bco-dmo.968453.1
title: Differential effects of temperature on multiple components of fitness in a modular animal (Bugula neritina) reveal how temperature affects reproductive capacity

description: Thermal performance curves (TPCs) are important tools for predicting the sensitivity of populations to climate change. The goal of this study was to assess and compare the relationships between temperature and different life-history components in a modular animal to reveal the mechanisms underlying TPCs for fitness. We reared replicated clones of the marine bryozoan Bugula neritina across a thermal gradient (16 values) ranging from 23 to 32°C, which reflected the upper thermal range of seasonal variation in the field. TPCs were constructed for survival (measured as zooids states within a colony), growth rate, development to reproductive maturity and reproductive capacity, which were measured over much of the realized lifespan expected under field conditions (~30 days). The effect of temperature was more acute on zooid states rather than whole-colony survival, and increased temperature increased the frequency of polypide regression. Most colonies reached reproductive maturity up to ~30°C, but growth rate and reproduction decreased at temperatures beyond ~25°C. The decline in reproductive capacity over temperatures above ~25°C was then due to the decline in the production of zooids capable of brooding embryos and zooids transitioning to regressed states up until about 30°C and transitioning to dead state beyond that.
DOI: 10.26008/1912/bco-dmo.968839.1
title: The effect of relatedness and density on survival and growth of Bugula neritina in the Gulf of Mexico, Florida from October to December 2021.

description: In the Gulf of Mexico, Florida, we performed a series of field experiments using an experimentally tractable species (the bryozoan Bugula neritina) to test the hypothesis that the density, spatial arrangement, and genetic relatedness of neighbours differentially affect survival, growth, reproduction, paternity, and sperm dispersal. We manipulated the density and relatedness of neighbours and found that increased density reduced survival but not growth rate, and that there was no effect of relatedness on survival, growth, or fecundity, in contrast to previous studies. We also manipulated the distances to the nearest neighbour and used genetic markers to assign paternity within known mother–offspring groups to estimate how proximity affects mating success. Distance to the nearest neighbour did not affect the number of settlers produced, the paternity share, or the degree of multiple paternity. Overall, larger than expected sperm dispersal led to high multiple paternity, regardless of the distance to the nearest neighbour.
DOI: 10.26008/1912/bco-dmo.969070.1
title: The effect of distance to the nearest neighbour on reproductive output and paternity of Bugula neritina in the Gulf of Mexico, Florida from April to June 2021.

description: In the Gulf of Mexico, Florida, we performed a series of field experiments using an experimentally tractable species (the bryozoan Bugula neritina) to test the hypothesis that the density, spatial arrangement, and genetic relatedness of neighbours differentially affect survival, growth, reproduction, paternity, and sperm dispersal. We manipulated the density and relatedness of neighbours and found that increased density reduced survival but not growth rate, and that there was no effect of relatedness on survival, growth, or fecundity, in contrast to previous studies. We also manipulated the distances to the nearest neighbour and used genetic markers to assign paternity within known mother–offspring groups to estimate how proximity affects mating success. Distance to the nearest neighbour did not affect the number of settlers produced, the paternity share, or the degree of multiple paternity. Overall, larger than expected sperm dispersal led to high multiple paternity, regardless of the distance to the nearest neighbour.
DOI: 10.15468/dl.gz8w2s
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Indonesia",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Issue is one of (Geodetic datum assumed WGS84, Country derived from coordinates)",
 "TaxonKey is Rhincodon typus",
 "Year 2020-2025"
 ]
}

 The dataset includes 13 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0021513-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.26008/1912/bco-dmo.957527.2
title: Open cell and single-step method for total alkalinity titrations from samples collected on R/V Mirai cruises MR23-06C and MR23-07 in the North Pacific and Western Arctic from September to November 2023

description: Duplicate samples were collected for total alkalinity on two cruises in 2023 aboard the R/V Mirai (MR23-06C and MR23-07) in order to compare two different methods of total alkalinity titrations: the open cell with non-linear least squares fitting and single step with spectrophotometric endpoint detection. Measurements of dissolved inorganic carbon and pH on the total scale were also made as quality control checks. GLODAPv2 hinted at a potential offset between laboratories in the Pacific Ocean. We hypothesized that if non-negligible amounts of organic alkalinity were present, the different methods could be titrating different amounts leading to differences between the methods. The results show that any offset is within the uncertainty of the analytical precision and presence of organic alkalinity in open ocean waters remains inconclusive.
DOI: 10.26008/1912/bco-dmo.968545.1
title: The effect of relatedness on survival, growth, and reproduction of Bugula neritina in the Gulf of Mexico, Florida from March to June 2022.

description: In the Gulf of Mexico, Florida, we performed a series of field experiments using an experimentally tractable species (the bryozoan Bugula neritina) to test the hypothesis that the density, spatial arrangement, and genetic relatedness of neighbours differentially affect survival, growth, reproduction, paternity, and sperm dispersal. We manipulated the density and relatedness of neighbours and found that increased density reduced survival but not growth rate, and that there was no effect of relatedness on survival, growth, or fecundity, in contrast to previous studies. We also manipulated the distances to the nearest neighbour and used genetic markers to assign paternity within known mother–offspring groups to estimate how proximity affects mating success. Distance to the nearest neighbour did not affect the number of settlers produced, the paternity share, or the degree of multiple paternity. Overall, larger than expected sperm dispersal led to high multiple paternity, regardless of the distance to the nearest neighbour.
DOI: 10.6084/m9.figshare.30565472
title: Units used for motif enrichment (20 bp)

description: The fasta used for the motif enrichment. The fasta were collected by the potential modified sites.
DOI: 10.6084/m9.figshare.30565472.v1
title: Units used for motif enrichment (20 bp)

description: The fasta used for the motif enrichment. The fasta were collected by the potential modified sites.
DOI: 10.26008/1912/bco-dmo.958920.1
title: Total aerosol iron isotope ratios from the 2010 GA03 U.S. GEOTRACES North Atlantic Transect Cruise, KN199-04, on R/V Knorr

description: This dataset includes total aerosol iron isotope ratios from the GA03 U.S. GEOTRACES North Atlantic Transect Cruise, KN199-04, on R/V Knorr which took place from October to November 2010.
DOI: 10.26008/1912/bco-dmo.958934.1
title: Total aerosol iron isotope ratios from the 2011 GA03 U.S. GEOTRACES North Atlantic Transect Cruise, KN204-01, on R/V Knorr

description: This dataset includes total aerosol iron isotope ratios from the GA03 U.S. GEOTRACES North Atlantic Transect Cruise, KN204-01, on R/V Knorr which took place from November to December 2011.
DOI: 10.26008/1912/bco-dmo.969971.1
title: Nutrient concentrations and biogeochemical rate data collected in the eastern tropical North Pacific Ocean in 2017 on R/V Oceanus cruise 1704A

description: This dataset contains nutrient concentrations and biogeochemical rate data collected in the eastern tropical North Pacific Ocean in 2017 on R/V Oceanus cruise 1704A.
DOI: 10.26008/1912/bco-dmo.881861.6
title: Zooplankton biomass measured from net tows conducted during ongoing monthly cruises, from April 1994 to December 2024, at the Bermuda Atlantic Time-series Study (BATS) site in the Sargasso Sea

description: This dataset includes measurements of zooplankton biomass from net tows conducted during ongoing monthly cruises, from April 1994 to December 2024, at the Bermuda Atlantic Time-series Study (BATS) site (31° 40' N 64° 10'W) in the Sargasso Sea. Mesozooplankton were collected by oblique tows using a rectangular frame net with 202 micrometer (µm) mesh. Samples from tows were immediately split on board for subsequent wet and dry weight measurements.
DOI: 10.26008/1912/bco-dmo.969102.1
title: Metabarcoding data from microbial diversity survey at Axial Seamount aboard the R/V Thompson cruise TN420 in Jul 2022 and Jul 2023

description: Microorganisms are the ancestral forms of life on our planet and have been instrumental in shaping all of Earth's environments into what they are today. In the ocean, microbial prokaryotes and eukaryotes form the foundation of marine food webs through their activity and interactions. Single-celled microbial eukaryotes (or protists) are some of the most important species on the planet, yet our understanding of how their activities influence and regulate the ocean ecosystem is poorly constrained. At deep-sea hydrothermal vents in particular, our understanding of microbial food web dynamics is incomplete without including the role of microbial eukaryotes. In July 2023, we completed an ROV expedition to Axial Seamount (NE Pacific), where we used ROV Jason to collect low temperature diffuse vent fluid and surrounding plume and background seawater. These samples were filtered in situ or shipboard to collect the microbial communities. Data provided are the product of amplicon tag-sequencing using both 18S rRNA and 16S rRNA gene primers.
DOI: 10.26008/1912/bco-dmo.969502.1
title: Concentrations of iron bound to humic-like substances and total iron-binding capacity of humic-like substances from discrete samples collected on the GP17-ANT RVIB Palmer cruise NBP24-01 in the Amundsen Sea from Nov 2023 to Jan 2024

description: This dataset includes the concentrations of iron (Fe) bound to humic-like substances and total iron-binding capacity of humic-like substances from discrete depth profile and towfish samples collected on the GP17-ANT cruise NBP24-01 on the R/V IB Nathaniel B. Palmer in the Amundsen Sea from 29 November 2023 to 28 January 2024.

This project investigates the impact of Fe-binding humic-like substances on Fe biogeochemistry in the Amundsen Sea. The project used a combination of depth profile and surface towfish samples to characterize the contributions of humic-like substances to Fe biogeochemistry across gradients in primary production, water masses, and bathymetric features in the Amundsen Sea. Understanding the distributions of Fe-binding humic-like substances provides insight into the impact of compositional changes of the Fe-binding ligand pool on Fe biogeochemistry and may help elucidate specific sources of these ligands.
DOI: 10.26008/1912/bco-dmo.924465.1
title: Acropora cervicornis 16S rRNA sequence metadata: nutrient- and disease-exposed from samples collected at  Mote Marine Laboratory in situ nursery from June to July 2022

description: In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote’s in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory’s experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote’s in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis.  We collected 190 samples for 16S rRNA sequencing, including prior to nutrient exposure, nutrient-exposed and -unexposed corals prior to disease challenge, and at several timepoints throughout disease exposure capturing both diseased and apparently healthy phenotypes. We additionally collected numerous samples for measurements of immune-related proteins. In brief, we found that disease challenge significantly impacted microbial communities, leading to significant differences in community composition between disease-exposed samples that developed disease, exposed samples that resisted disease, and healthy unexposed controls. The taxa Algicola, Halarcobacter, Saprospira, Shimia, Tenacibaculum, Thalassolituus, and Thalassotalea increased in abundance in samples that developed disease. Of the genotypes chosen for the experiment, one genotype was dominated by the putative parasite Aquarickettsia. We found that both genotypes were susceptible to disease, in contrast to previous results indicating that genotypes with low abundance of Aquarickettsia and relatively diverse microbiomes were disease-resistant. Raw sequence reads have been submitted to SRA under PRJNA1024453 with an embargo until publication.
DOI: 10.26008/1912/bco-dmo.955717.2
title: Bottle data from CTD profiles from the ODF rosette deployed on the US GEOTRACES GP17-OCE cruise on R/V Roger Revelle (RR2214) from December 2022 to January 2023

description: This dataset includes the processed bottle data from CTD profiles of the ODF rosette deployed on the US GEOTRACES GP17-OCE cruise on R/V Roger Revelle (RR2214) from December 2022 to January 2023. Data were processed by the Oceanographic Data Facility (ODF) at Scripps Institution of Oceanography. The data include bottle salinity and oxygen, in addition to CTD measurements (oxygen, salinity, temperature, pressure).
DOI: 10.26008/1912/bco-dmo.924466.1
title: Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at  Mote Marine Laboratory in situ nursery from June to July 2022

description: In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote’s in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory’s experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote’s in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis.  To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, a number of immune related proteins and antioxidants were measured using microplate assays from a total of 55 samples taken prior to disease exposure and following disease exposure as corals either resisted or developed disease. Total host protein and the antioxidant superoxide dismutase were found to be higher in healthy corals regardless of genotype or nutrient enrichment. Phenoloxidase and prophenoloxidase were higher in concentration in diseased samples compared to healthy samples. Disease-exposed but apparently healthy corals had higher superoxide dismutase, prophenoloxidase, and peroxidase activity than either healthy unexposed corals or diseased corals despite total protein concentrations that were lower than healthy unexposed corals.
DOI: 10.26008/1912/bco-dmo.933861.2
title: Nutrients and bottle oxygen measured by the Scripps ODF group on the US GEOTRACES GP17-OCE cruise on R/V Roger Revelle (RR2214) from December 2022 to January 2023 in the South Pacific and Southern Oceans

description: This dataset includes the nutrients and bottle oxygen measurements made by the Ocean Data Facility (ODF) group of  Scripps Institution of Oceanography during the US GEOTRACES GP17-OCE cruise. The cruise took place on R/V Roger Revelle (cruise ID RR2214) from December 2022 to January 2023 in the South Pacific and Southern Oceans.
DOI: 10.26008/1912/bco-dmo.964619.1
title: Microbial fluid cell count data collected on Falkor (too), cruise Fkt231202 with ROV fluid sampler in the Central Eastern Pacific offshore Costa Rica in Dec 2023

description: These data are microscopic cell counts of microbes in venting fluids and background seawater samples collected during Octopus Odyssey (too) expedition on Falkor (too), cruise Fkt231202, in the Central Eastern Pacific from December 4th to December 12th, 2023. Instrument used to collect the sampler was a SUPR (Suspended Underwater Particulate Rosette) sampler modified for ROV SuBastian. Cells were visualized using SYBR staining. Samples were collected by Julie Huber.