DOI: 10.22002/ntk1n-50z96
title: JV1181

description: Legionella thyA ΔdotUF  (JV1181)
description: <strong>Tilt Series Date:</strong> 2017-02-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/rawdata/</p>JV1181_02022017a0007.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/rawdata/JV1181_02022017a0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV1181_02022017a0007_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/3dimage_105465/JV1181_02022017a0007_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-02-02-13_slicer18943.png,  Have seen this in many many Legionella tomograms. That membrane strip looks like coated with proteins., 515.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-02-02-13_slicer18943.png"
        > <i class="download icon"></i> Download </a></p>

        JV1181_02022017a0007.ali,  434.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/file_21120/JV1181_02022017a0007.ali"
        > <i class="download icon"></i> Download </a></p>

        JV1181_02022017a0007.tlt,  488.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/file_21121/JV1181_02022017a0007.tlt"
        > <i class="download icon"></i> Download </a></p>

        T4SSp.mod,  1.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/file_21122/T4SSp.mod"
        > <i class="download icon"></i> Download </a></p>

        T4SSp_2k.mod,  1.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-02-02-13/file_21123/T4SSp_2k.mod"
        > <i class="download icon"></i> Download </a></p>