DOI: 10.22002/81xvy-m7k27
title: Wild type NA1000

description: This is the cell shown in Fig. 2(E-E3) and Fig. 5 of Li et al. 2007.Keywords: Division site. FtsZ filaments.
description: <strong>Tilt Series Date:</strong> 2006-06-07</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, Amira</p>
<strong>Collaborators and Roles:</strong> Cells were cultured by Zhuo Li. Data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle. 10 ug/ml A22 was added to depolymerize MreB filaments.</p>
<strong>Sample Preparation:</strong> 4 ul of cell culture at different time points were mixed with 10nm colloidal gold and plunge-frozen onto glow-discharged Quantifoil R2/2, R2/1, or R1.2/1.3 grids using a Vitrobot (FEI).</p>
  Fig3a22wtamira.jpg: Segmentation pg_1.jpg: Segmentation pg_closrup.jpg: Segmentation
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-06-07-2/3dimage_693/</p>a22wt690660w1d12u454c03_fixed_bin.mrc.rec.mrc,  Reconstruction (Pixel Size 1.34 nm), 179.7 MB  
        
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        da22wt690660w1d12u454c03_fixed_bin.mrc.rec_0015.mrc,  Denoised subtomogram, 179.7 MB  
        
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        a22wt690660w1d12u454c03.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.0 GB  
        
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        a22wt690660w1d12u454c03_fixed_bin.mrc_full.rec,  Reconstruction (Pixel Size 1.34 nm), 943.7 MB  
        
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        da22wt690660w1d12u454c03trim.am,  Segmentation, 49.3 MB  
        
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        da22wt690660w1d12u454c03trim-labels.am,  Segmentation, 3.0 MB  
        
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        da22wt690660w1d12u454c03trim-labels1.am,  Segmentation, 2.5 MB  
        
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        da22wt690660w1d12u454c03trim-labelsFL.am,  Segmentation, 779.7 kB  
        
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        da22wt690660w1d12u454c03trim-labelsFL.surf,  Segmentation, 261.1 kB  
        
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        da22wt690660w1d12u454c03trim-labels_IM.am,  Segmentation, 1.2 MB  
        
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        da22wt690660w1d12u454c03trim-labels_IM.surf,  Segmentation, 21.0 MB  
        
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        da22wt690660w1d12u454c03trim-labels_IM1.am,  Segmentation, 1.1 MB  
        
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        da22wt690660w1d12u454c03trim-labelsOM.am,  Segmentation, 1.2 MB  
        
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        da22wt690660w1d12u454c03trim-labelsOM.surf,  Segmentation, 24.1 MB  
        
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        da22wt690660w1d12u454c03trim-labelsOM_SLay.am,  Segmentation, 2.2 MB  
        
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        da22wt690660w1d12u454c03trim-labelsOUTlayer.am,  Segmentation, 1.5 MB  
        
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        da22wt690660w1d12u454c03trim-labels_PEP.am,  Segmentation, 1.1 MB  
        
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        da22wt690660w1d12u454c03trim-labels_PEP.surf,  Segmentation, 19.0 MB  
        
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        da22wt690660w1d12u454c03trim-labelsSlays.am,  Segmentation, 1.6 MB  
        
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        da22wt690660w1d12u454c03trim-labelsSlays.surf,  Segmentation, 42.6 MB  
        
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        Fig3a22wt.hx,  Segmentation, 5.8 kB  
        
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        Fig3a22wtFL.surf,  Segmentation, 261.2 kB  
        
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        Fig3a22wtIM.am,  Segmentation, 1.1 MB  
        
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        Fig3a22wtIM.surf,  Segmentation, 317.0 kB  
        
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        Fig3a22wtOM.am,  Segmentation, 1.2 MB  
        
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        Fig3a22wtOM.surf,  Segmentation, 3.2 MB  
        
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