cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6886499.v1
title: Underexplored regions of Pakistan yield five new species of <i>Leucoagaricus</i>

description: The large genus <i>Leucoagaricus</i> (Basidiomycota) is poorly studied in Pakistan, where the northern parts of the country are considered hot spots for biodiversity. Based on morphological and molecular data, five new species are described: <i>Leucoagaricus badius, L. lahorensiformis, L. pakistaniensis, L. sultanii</i>, and <i>L. umbonatus</i>. Descriptions and illustrations are given for the new taxa. A phylogeny based on morphology and four DNA regions, including the internal transcribed spacers (ITS1-5.8S-ITS2 = ITS) and D1–D2 domains of the 28S gene of the nuc rDNA, the gene for RNA polymerase II second largest subunit (<i>RPB2</i>), and the translation elongation factor 1-α gene (<i>TEF1-α</i>), shows that the new taxa are clustered in a clade representing <i>Leucoagaricus</i> section <i>Rubrotincti</i> subgenus <i>Sericeomyces</i>.
DOI: 10.7910/dvn/debtui/t6ci2n
title: 1-33.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.6084/m9.figshare.6886493
title: Inhabiting plant roots, nematodes, and truffles—<i>Polyphilus</i>, a new helotialean genus with two globally distributed species

description: Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode <i>Heterodera filipjevi</i>. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nuc rDNA (18S), and partial RNA polymerase II second-largest subunit (<i>RPB2</i>), were amplified and sequenced for molecular phylogenetic analyses. Analyses of similar ITS sequences from public databases revealed two globally distributed lineages detected in several biomes from different geographic regions. The host interaction of isolates from nematodes was examined using in vitro bioassays, which revealed that the fungi could penetrate nematode cysts and colonize eggs of <i>H. filipjevi</i>, confirming observations from field-collected samples. This is the first report of a DSE, and we are not aware of other helotialean fungal species colonizing the eggs of a plant-parasitic nematode. Neither conidiomata and conidia nor ascomata formation was detected in any of the isolates. Based on molecular phylogenetic analyses, these isolates represent a distinct lineage within the Helotiales in the Hyaloscyphaceae. For this lineage, we propose here the new genus <i>Polyphilus</i> represented by two new species, <i>P. sieberi</i> and <i>P. frankenii</i>.
DOI: 10.6084/m9.figshare.6886496.v1
title: Growth medium and incubation temperature alter the <i>Pseudogymnoascus destructans</i> transcriptome: implications in identifying virulence factors

description: <i>Pseudogymnoascus destructans</i> is the causal agent of bat white-nose syndrome (WNS), which is devastating some North American bat populations. Previous transcriptome studies provided insight regarding the molecular mechanisms involved in WNS; however, it is unclear how different environmental parameters could influence pathogenicity. This information could be useful in developing management strategies to mitigate the negative impacts of <i>P. destructans</i> on bats. We cultured three <i>P. destructans</i> isolates from Atlantic Canada on two growth media (potato dextrose agar and Sabouraud dextrose agar) that differ in their nitrogen source, and at two separate incubation temperatures (4 C and 15 C) that approximate the temperature range of bat hibernacula during the winter and a temperature within its optimal mycelial growth range. We conducted RNA sequencing to determine transcript levels in each sample and performed differential gene expression (DGE) analyses to test the influence of growth medium and incubation temperature on gene expression. We also compared our in vitro results with previous RNA-sequencing data sets generated from <i>P. destructans</i> growing on the wings of a susceptible host, <i>Myotis lucifugus</i>. Our findings point to a critical role for substrate and incubation temperature in influencing the <i>P. destructans</i> transcriptome. DGE analyses suggested that growth medium plays a larger role than temperature in determining <i>P. destructans</i> gene expression and that although the psychrophilic fungus responds to different nitrogen sources, it may have evolved for continued growth at a broad range of low temperatures. Further, our data suggest that down-regulation of the RNA-interference pathway and increased fatty acid metabolism are involved in the <i>P. destructans</i>–bat interaction. Finally, we speculate that to reduce the activation of host defense responses, <i>P. destructans</i> minimizes changes in the expression of genes encoding secreted proteins during bat colonization.
DOI: 10.7910/dvn/debtui/hdbwvg
title: 1-58.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.6084/m9.figshare.6886490
title: <i>Talaromyces borbonicus</i>, sp. nov., a novel fungus from biodegraded <i>Arundo donax</i> with potential abilities in lignocellulose conversion

description: A novel fungal species able to synthesize enzymes with potential synergistic actions in lignocellulose conversion was isolated from the biomass of <i>Arundo donax</i> during biodegradation under natural conditions in the Gussone Park of the Royal Palace of Portici (Naples, Italy). In this work, this species was subjected to morphological and phylogenetic analyses. Sequencing of its genome was performed, resulting in 28 scaffolds that were assembled into 27.05 Mb containing 9744 predicted genes, among which 396 belong to carbohydrate-active enzyme (CAZyme)-encoding genes. Here we describe and illustrate this previously unknown species, which was named <i>Talaromyces borbonicus</i>, by a polyphasic approach combining phenotypic, physiological, and sequence data.
DOI: 10.6084/m9.figshare.6886490.v1
title: <i>Talaromyces borbonicus</i>, sp. nov., a novel fungus from biodegraded <i>Arundo donax</i> with potential abilities in lignocellulose conversion

description: A novel fungal species able to synthesize enzymes with potential synergistic actions in lignocellulose conversion was isolated from the biomass of <i>Arundo donax</i> during biodegradation under natural conditions in the Gussone Park of the Royal Palace of Portici (Naples, Italy). In this work, this species was subjected to morphological and phylogenetic analyses. Sequencing of its genome was performed, resulting in 28 scaffolds that were assembled into 27.05 Mb containing 9744 predicted genes, among which 396 belong to carbohydrate-active enzyme (CAZyme)-encoding genes. Here we describe and illustrate this previously unknown species, which was named <i>Talaromyces borbonicus</i>, by a polyphasic approach combining phenotypic, physiological, and sequence data.
DOI: 10.6084/m9.figshare.6886493.v1
title: Inhabiting plant roots, nematodes, and truffles—<i>Polyphilus</i>, a new helotialean genus with two globally distributed species

description: Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode <i>Heterodera filipjevi</i>. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nuc rDNA (18S), and partial RNA polymerase II second-largest subunit (<i>RPB2</i>), were amplified and sequenced for molecular phylogenetic analyses. Analyses of similar ITS sequences from public databases revealed two globally distributed lineages detected in several biomes from different geographic regions. The host interaction of isolates from nematodes was examined using in vitro bioassays, which revealed that the fungi could penetrate nematode cysts and colonize eggs of <i>H. filipjevi</i>, confirming observations from field-collected samples. This is the first report of a DSE, and we are not aware of other helotialean fungal species colonizing the eggs of a plant-parasitic nematode. Neither conidiomata and conidia nor ascomata formation was detected in any of the isolates. Based on molecular phylogenetic analyses, these isolates represent a distinct lineage within the Helotiales in the Hyaloscyphaceae. For this lineage, we propose here the new genus <i>Polyphilus</i> represented by two new species, <i>P. sieberi</i> and <i>P. frankenii</i>.
DOI: 10.6084/m9.figshare.6886487
title: Multilocus phylogenetic analysis of <i>Talaromyces</i> species isolated from cucurbit plants in China and description of two new species, <i>T. cucurbitiradicus</i> and <i>T. endophyticus</i>

description: During a survey of endophytic fungi from cucurbit plants in China, 21 <i>Talaromyces</i> strains were isolated from ten symptomless plants. Phylogenetic analysis of the partial RNA polymerase II largest subunit gene (<i>RPB2</i>) showed that the strains belong to <i>Talaromyces</i> sections <i>Talaromyces</i> and <i>Islandici</i>. Based on morphological characters and multilocus phylogenetic analysis of the nuc rDNA internal transcribed spacer region (ITS1-5.8S-ITS2 = ITS), calmodulin (<i>CaM</i>), and β-tubulin (<i>TUB</i>) genes, the strains were identified as four known species, <i>T. cnidii, T. pinophilus, T. radicus</i>, and <i>T. wortmannii</i>, and two new species. Two new species, <i>T. cucurbitiradicus</i> from pumpkin roots and <i>T. endophyticus</i> from cucumber stems, are described in this study. <i>Talaromyces cucurbitiradicus</i> is morphologically similar to <i>T. funiculosus</i> but differs in the number of phialides per metula and by the production of chlamydospores. <i>Talaromyces endophyticus</i> is morphologically similar to <i>T. cerinus</i> and <i>T. chlamydosporus</i> but differs by producing yellowish colonies and by lacking chlamydospores. Further analyses of polymorphisms in ITS and <i>TUB</i> sequences supported the distinctions among <i>T. cucurbitiradicus, T. endophyticus</i>, and similar species.
DOI: 10.7910/dvn/debtui/gvdfhn
title: 1-18.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.6084/m9.figshare.6886484
title: Genetic variability of <i>Ganoderma zonatum</i> infecting palms in Florida

description: <i>Ganoderma zonatum</i> is a lethal pathogen of palms (Arecaceae) in Florida (USA) because it degrades the wood of the lowest section of the palm trunk. This fungus is widespread throughout Florida, where it has been observed on over 60 species of palms. The authors examined the genetic variability of 25 isolates of <i>G. zonatum</i> obtained in Florida from 12 different palm species and representing 17 unique property locations in eight counties to determine if <i>G. zonatum</i> represents a species complex. The three genomic regions examined were the nuc rDNA ITS1-5.8S-ITS2 region (ITS), the coding region for RNA polymerase II subunit 2 (<i>rpb2</i>) domains 6 and 7, and the partial gene for translation elongation factor 1α (<i>tef1α</i>). The results indicated that variability among these three genomic regions was minimal, and the variability observed was not related to palm host or geographic region within Florida. Thus, in the geographic region surveyed, <i>G. zonatum</i> does not appear to represent a species complex.
DOI: 10.6084/m9.figshare.6886487.v1
title: Multilocus phylogenetic analysis of <i>Talaromyces</i> species isolated from cucurbit plants in China and description of two new species, <i>T. cucurbitiradicus</i> and <i>T. endophyticus</i>

description: During a survey of endophytic fungi from cucurbit plants in China, 21 <i>Talaromyces</i> strains were isolated from ten symptomless plants. Phylogenetic analysis of the partial RNA polymerase II largest subunit gene (<i>RPB2</i>) showed that the strains belong to <i>Talaromyces</i> sections <i>Talaromyces</i> and <i>Islandici</i>. Based on morphological characters and multilocus phylogenetic analysis of the nuc rDNA internal transcribed spacer region (ITS1-5.8S-ITS2 = ITS), calmodulin (<i>CaM</i>), and β-tubulin (<i>TUB</i>) genes, the strains were identified as four known species, <i>T. cnidii, T. pinophilus, T. radicus</i>, and <i>T. wortmannii</i>, and two new species. Two new species, <i>T. cucurbitiradicus</i> from pumpkin roots and <i>T. endophyticus</i> from cucumber stems, are described in this study. <i>Talaromyces cucurbitiradicus</i> is morphologically similar to <i>T. funiculosus</i> but differs in the number of phialides per metula and by the production of chlamydospores. <i>Talaromyces endophyticus</i> is morphologically similar to <i>T. cerinus</i> and <i>T. chlamydosporus</i> but differs by producing yellowish colonies and by lacking chlamydospores. Further analyses of polymorphisms in ITS and <i>TUB</i> sequences supported the distinctions among <i>T. cucurbitiradicus, T. endophyticus</i>, and similar species.
DOI: 10.6084/m9.figshare.6886484.v1
title: Genetic variability of <i>Ganoderma zonatum</i> infecting palms in Florida

description: <i>Ganoderma zonatum</i> is a lethal pathogen of palms (Arecaceae) in Florida (USA) because it degrades the wood of the lowest section of the palm trunk. This fungus is widespread throughout Florida, where it has been observed on over 60 species of palms. The authors examined the genetic variability of 25 isolates of <i>G. zonatum</i> obtained in Florida from 12 different palm species and representing 17 unique property locations in eight counties to determine if <i>G. zonatum</i> represents a species complex. The three genomic regions examined were the nuc rDNA ITS1-5.8S-ITS2 region (ITS), the coding region for RNA polymerase II subunit 2 (<i>rpb2</i>) domains 6 and 7, and the partial gene for translation elongation factor 1α (<i>tef1α</i>). The results indicated that variability among these three genomic regions was minimal, and the variability observed was not related to palm host or geographic region within Florida. Thus, in the geographic region surveyed, <i>G. zonatum</i> does not appear to represent a species complex.
DOI: 10.7910/dvn/debtui/gqgk4t
title: 1-54.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.7910/dvn/debtui/sjfub4
title: 1-11.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.7910/dvn/debtui/2h1fim
title: 1-55.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.7910/dvn/debtui/ycmu5e
title: 1-40.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.6084/m9.figshare.6886469
title: Sense of space: Tactile sense for exploratory behavior of roots

description: In soil, plant roots grow in heterogeneous environments. Plant roots are always facing the difficulty of searching effectively the patchy natural resources, such as water, oxygen, ions and mineral nutrition. Numerous studies reported that root apex navigation enables roots to explore complex environments. In this short communication, we characterize how growing maize roots explore narrow space available with two experimental settings: tactile exploration of narrow glass tube and circumnutation in free space. We also discuss root growth in the soil in terms of foraging behavior guided by the sensory root apex.
DOI: 10.6084/m9.figshare.6886469.v1
title: Sense of space: Tactile sense for exploratory behavior of roots

description: In soil, plant roots grow in heterogeneous environments. Plant roots are always facing the difficulty of searching effectively the patchy natural resources, such as water, oxygen, ions and mineral nutrition. Numerous studies reported that root apex navigation enables roots to explore complex environments. In this short communication, we characterize how growing maize roots explore narrow space available with two experimental settings: tactile exploration of narrow glass tube and circumnutation in free space. We also discuss root growth in the soil in terms of foraging behavior guided by the sensory root apex.
DOI: 10.7910/dvn/debtui/apdwhm
title: 1-13.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.7910/dvn/debtui/w1kbk5
title: 1-32.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.7910/dvn/debtui/xjnepu
title: 1-53.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.6084/m9.figshare.6886460
title: <i>Herpomyces</i> ectoparasitic fungi (Ascomycota, Laboulbeniales) are globally distributed by their invasive cockroach hosts and through the pet trade industry

description: The introduction of non-native animals occasionally results in the co-introduction of their microbial symbionts or parasites. The trade of exotic pets and zoo animals has inadvertently introduced several parasitic species to countries where they are non-native. Both the presence of suitable native hosts and opportunity for dispersal determine whether these non-native species become naturalized. During our studies dealing with species of <i>Herpomyces</i> (Ascomycota, Laboulbeniomycetes), fungi that are exclusively ectoparasitic on cockroaches (Hexapoda, Blattodea), we make use of artificial colonies. Most of our specimens originate from pet stores and laboratory populations. Although they were originally intended for transmission studies, we discovered that some cockroaches from artificial colonies carried fruiting bodies of <i>Herpomyces</i>. We screened a total 292 cockroaches from 11 populations that we maintained after purchase. Sources were different pet stores, a toxicological laboratory, and a biological supply company. In eight populations, we found at least some <i>Herpomyces</i>-infected cockroaches. Parasite prevalence varied between 8.77% and 86.33%. Host associations were <i>Blatta orientalis</i> with <i>Herpomyces stylopygae, Blattella germanica</i> with <i>H. ectobiae, Periplaneta americana</i> with <i>H. periplanetae, Phoetalia pallida</i> with <i>H. leurolestis</i>, and <i>Shelfordella lateralis</i> with an undescribed species of <i>Herpomyces</i>. Apart from the new reports, host associations, and consequences for taxonomy (a new species based on morphological and molecular characters), we started to think about the geographic distributions of these fungi and how we, humans, shape them through spreading hosts and through international pet trade. We reviewed the currently known records of <i>Herpomyces</i>-associated cockroaches and host-parasite relationships. Based on the available data, on a global scale, at least half of the currently known species of <i>Herpomyces</i> are spread by globally invasive host species and through international pet trade. This indicates that the distribution and host range of these obscure and often unnoticed fungi are affected by human activities.
DOI: 10.7910/dvn/debtui/6rq3g8
title: 1-34.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;
DOI: 10.6084/m9.figshare.6886460.v1
title: <i>Herpomyces</i> ectoparasitic fungi (Ascomycota, Laboulbeniales) are globally distributed by their invasive cockroach hosts and through the pet trade industry

description: The introduction of non-native animals occasionally results in the co-introduction of their microbial symbionts or parasites. The trade of exotic pets and zoo animals has inadvertently introduced several parasitic species to countries where they are non-native. Both the presence of suitable native hosts and opportunity for dispersal determine whether these non-native species become naturalized. During our studies dealing with species of <i>Herpomyces</i> (Ascomycota, Laboulbeniomycetes), fungi that are exclusively ectoparasitic on cockroaches (Hexapoda, Blattodea), we make use of artificial colonies. Most of our specimens originate from pet stores and laboratory populations. Although they were originally intended for transmission studies, we discovered that some cockroaches from artificial colonies carried fruiting bodies of <i>Herpomyces</i>. We screened a total 292 cockroaches from 11 populations that we maintained after purchase. Sources were different pet stores, a toxicological laboratory, and a biological supply company. In eight populations, we found at least some <i>Herpomyces</i>-infected cockroaches. Parasite prevalence varied between 8.77% and 86.33%. Host associations were <i>Blatta orientalis</i> with <i>Herpomyces stylopygae, Blattella germanica</i> with <i>H. ectobiae, Periplaneta americana</i> with <i>H. periplanetae, Phoetalia pallida</i> with <i>H. leurolestis</i>, and <i>Shelfordella lateralis</i> with an undescribed species of <i>Herpomyces</i>. Apart from the new reports, host associations, and consequences for taxonomy (a new species based on morphological and molecular characters), we started to think about the geographic distributions of these fungi and how we, humans, shape them through spreading hosts and through international pet trade. We reviewed the currently known records of <i>Herpomyces</i>-associated cockroaches and host-parasite relationships. Based on the available data, on a global scale, at least half of the currently known species of <i>Herpomyces</i> are spread by globally invasive host species and through international pet trade. This indicates that the distribution and host range of these obscure and often unnoticed fungi are affected by human activities.
DOI: 10.7910/dvn/debtui/x8uygt
title: 1-31.5V

description: sigma=1.5Volts; This is a FITS file with 1 (primary) HDU. The following recognized metadata keys have been found in the FITS file: NAXIS2; NAXIS; NAXIS1; NAXIS0;