cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0sn07sb
title: CIL:35969, Notophthalmus, sperm. In Cell Image Library

description: Figures 338 (upper) and 339 (lower) from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Transverse sections through midpiece and principal piece of several sperm from the newt, Notophthalmos viridescens. (From Fawcett, Biol. Reprod. [Supp. 2, 90-127, 1970.]) A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j0rj4h83
title: CIL:35967, Neotragus moschatus, Mus musculus, sperm. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figures 331 (left) and 332 (right) from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Left, longitudinal section of midpiece from sperm of the suni antelope; right, longitudinal section through the principal piece of mouse sperm. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j04m93b1
title: CIL:35974, Felis catus, cardiac muscle cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 351 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Glycogen in the ventricle papillary muscle of cat heart. In this tissue, the glycogen occurs as individual particles in close association with mitochondria and the sacroplasmic reticulum, whereas only a few particles occur within the contractile material. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j0xw4hrc
title: CIL:35971, damselfly, flight muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: unembedded tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: Negatives scanned at LBL then reduced in size for upload TEM magnifications not currently available. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron.

Heads and tail were removed, the thorax was bisected and immersed in 100 mM KCl, 10 mM EDTA, 10 Triton X for a few hrs, to depolarize and skin the fibers, then maintained at -20 C in 50% glycerol in the same solution for several weeks to induce rigor by eliminating ATP. The muscle was gently homogenized in a waring blender in 0.9% NaCl. The fibrils were deposited on glass and sequentially fixed in 0.1% glutaraldehyde in 0.1 M cacodylate buffer, rinsed in 100 mM ammonium acetate, treated with 1% uranyl acetate for 30 seconds, rinsed in 40% methanol and frozen in liquid nitrogen. The fibrils were freeze dried at -90 C for 30 minutes and rotary shadowed with platinum either at 25C or 45C in a Balzer's 400 freeze fracture. The glass was dissolved in hydrofluoric acid and the replicas were mounted on copper grids and examined in a Philips 410 Microscope.
description: The image shows the surface of the ribbon-like myofibrils, with some remnants of the SR attached mostly at the Z lines. The regular disposition of attached cross bridges is visible in many areas. Most images of this group show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. 
 
References for the image: 
Loesser, K.E. and Franzini-Armstrong, C. A simple method for freeze-drying of macromolecules and macromolecular complexes. J. Struct. Biol. 103: 48-56, 1990. Bard, F., Franzini-Armstrong, C. and Ip W. Rigor cross bridges are double headed in fast muscle from crayfish. J. Cell Biol. 105: 2225-2234, 1987.
DOI: 10.6075/j0542mch
title: CIL:35961, Trichoptera sp. PACaddisfly-1, sperm. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 340 from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Cross sections of sperm tails of a caddis fly illustrate differences between the organization of the insect sperm flagella and that of mammals, notably in the position of the nine outer elements in relation to the doublets of the axoneme. Image by David Phillips. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j0zs2v96
title: CIL:35959, Cricetulus griseus, sperm. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 330 from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. This electron micrograph shows a transverse section through the principle piece of Chinese hamster spermatozoa. Image by David Phillips. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j0nc5zz5
title: CIL:35957, Leporidae, sperm. In Cell Image Library

description: Preparation: critical_point dried specimen
Relation to intact cell: whole mounted tissue
Item type: scanning electron microscopy (SEM)
Imaging mode: illumination by electrons; secondary_electron imaging
Parameter imaged: secondary electron generation
Data qualification: Processed
description: Figure 328 from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Scanning electron micrograph of rabbit spermatozoa on the endometrium of the uterus. Image by David Phillips. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j03778s2
title: CIL:35915, Saccharomyces cerevisiae. In Cell Image Library

description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. 
 
 TEM1 is a GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis. 
 
These phase,gfp images are part of an image group that ranges from CIL:35900-35919. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of electrons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
DOI: 10.6075/j04749xf
title: CIL:35909, Saccharomyces cerevisiae. In Cell Image Library

description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. 
 
 TEM1 is a GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis. 
 
These phase,gfp images are part of an image group that ranges from CIL:35900-35919. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of electrons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
DOI: 10.15468/dl.kmcw2b
title: Occurrence Download

description: A dataset containing 942 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Detarium senegalense"
 ]
}

 The dataset includes 942 records from 83 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009437-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0m61k83
title: CIL:35911, Saccharomyces cerevisiae. In Cell Image Library

description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. 
 
 TEM1 is a GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis. 
 
These phase,gfp images are part of an image group that ranges from CIL:35900-35919. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of electrons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
DOI: 10.6075/j0028qg4
title: CIL:35954, damselfly, flight muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: unembedded tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
description: Most images show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images.This image is part of the group image CFA00512. TEM magnification 24400X. The negative film was scanned at LBL then reduced in size for upload. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer. pH 7.2. stored at 4C for several days. Muscle dissected out. Infiltrated in 30% glycerol in water. Frozen in liquid nitrogen cooled Freon, fractured in Balzers 400 Freeze-fracture, shadowed with platinum at 45o angle. Replicas were cleaned with Clorox (to remove tissue and with either sulfuric acid or NaOH to try removing the tracheolae. Replicas examined in a Philips 410 Microscope. Reference for these images: 
 Takekura, H. and Franzini-Armstrong C. The structure of Ca2+ release units in arthropod body muscle indicates an indirect mechanism for excitation-contraction coupling. Biophys. J. 83, 83, 2742-2753, 2002.
DOI: 10.6075/j08051jd
title: CIL:35952, damselfly, flight muscle cell. In Cell Image Library

description: Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope. Reference for the image:Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199
description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: digital negative film
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0dv1hvk
title: CIL:35956, damselfly, flight muscle cell. In Cell Image Library

description: Negatives scanned at LBL then reduced in size for upload TEM magnifications not currently available. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron.

Heads and tail were removed, the thorax was bisected and immersed in 100 mM KCl, 10 mM EDTA, 10 Triton X for a few hrs, to depolarize and skin the fibers, then maintained at -20 C in 50% glycerol in the same solution for several weeks to induce rigor by eliminating ATP. The muscle was gently homogenized in a waring blender in 0.9% NaCl. The fibrils were deposited on glass and sequentially fixed in 0.1% glutaraldehyde in 0.1 M cacodylate buffer, rinsed in 100 mM ammonium acetate, treated with 1% uranyl acetate for 30 seconds, rinsed in 40% methanol and frozen in liquid nitrogen. The fibrils were freeze dried at -90 C for 30 minutes and rotary shadowed with platinum either at 25C or 45C in a Balzer's 400 freeze fracture. The glass was dissolved in hydrofluoric acid and the replicas were mounted on copper grids and examined in a Philips 410 Microscope.
description: The image shows the surface of the ribbon-like myofibrils, with some remnants of the SR attached mostly at the Z lines. The regular disposition of attached cross bridges is visible in many areas. Most images of this group show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. 
 
References for the image: 
Loesser, K.E. and Franzini-Armstrong, C. A simple method for freeze-drying of macromolecules and macromolecular complexes. J. Struct. Biol. 103: 48-56, 1990. Bard, F., Franzini-Armstrong, C. and Ip W. Rigor cross bridges are double headed in fast muscle from crayfish. J. Cell Biol. 105: 2225-2234, 1987.
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: unembedded tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j05719t8
title: CIL:35985, Batrachoseps attenuatus, hepatocyte. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 364 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A portion of a liver cell from the slender salamander, Batrachoseps attenuatus. This striking example of crystalline inclusion accumulation varies in the liver cells of the slender salamander by individual, and by season. The inclusions are often found near to endoplasmic reticulum. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j01j98km
title: CIL:35988, Homo sapiens, Sertoli cell. In Cell Image Library

description: Figure 367 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Filament-like crystalloid of Charcot-Bottcher in a human Sertoli cell. Image from Nagano, T., Z. Zellforsch, 73:89-106, 1966. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j03f4nf2
title: CIL:35981, Spermophilus citellus, Leydig cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 360 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Leydig cell from a reproductively active ground squirrel, Citellus lateralis. Physiological state can influence the extent to which lipid is stored in the same cell type. Here, lipid droplets are abundant, but during the quiescent phase of the breeding cycle, these cells would contain only one or two enormous lipid droplets that would occupy much of the cell body. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j0gx49dq
title: CIL:35989, Nicotiana tabacum, plant cell. In Cell Image Library

description: Figure 368 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A microbody or peroxisome from a cell in a leaf of tobacco (Nicotiana tabacum). Crystals within microbodies, or peroxisomes, are common, and these organelles appear in plant, as well as animal, cells. Image from Frederick, S.E., Newcomb, E.H., Science 163:1353, 1969; copyright held by AAAS. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.34740/kaggle/dsv/13565644
title: World Weather Repository ( Daily Updating )
DOI: 10.6075/j0sb44kt
title: CIL:35972, Phodopus, hepatocyte. In Cell Image Library

description: Figure 349 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Electron micrograph of an hepatic cell from a fasted hamster after an injection of cortisone. In mammalian liver, glycogen appears as coarse rosettes and tends to be associated with the smooth endoplasmic reticulum. Image by Albert Jones. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j09c6w8n
title: CIL:35966, Neotragus moschatus, Chinchilla lanigera, sperm. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figures 333 (left) and 334 (right) from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Left, longitudinal section of the junction of midpiece and principle piece from sperm of the chinchilla; right, comparable region of spermatozoon of the suni antelope. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.
DOI: 10.6075/j04j0cxv
title: CIL:35964, Cecidomyiinae, sperm. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
description: Figure 346 from Chapter 14 (Sperm Flagellum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Top panel: A cross section of sperm tail of the cecidomyid fly, Diplolaboncus tumorificus shows mitochondria scattered among doublets of microtubules. Lower panel, sperm tail organization in a related species of fly shows about 100 doublets a spiral or row-like arrangement. Figure from Baccetti and Dallai, J. Ultrastr. Res. 55: 50-69, 1976. A PDF copy of the accompanying chapter is available on the ASCB‚Äôs BioEDUCATE website.