DOI: 10.22002/qnm0j-qkh89
title: Wild type NA1000

description: This is the cell shown in Fig.1 and Fig.2(C-C3) of Li et al. 2007.
description: <strong>Tilt Series Date:</strong> 2006-03-23</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, Amira</p>
<strong>Collaborators and Roles:</strong> Cells were cultured by Zhuo Li. Data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle.</p>
<strong>Sample Preparation:</strong> 4 ul of cell culture at different time points were mixed with 10nm colloidal gold and plunge-frozen onto glow-discharged Quantifoil R2/2, R2/1, or R1.2/1.3 grids using a Vitrobot (FEI).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-3/3dimage_661/</p>dwt75min34k05.rec_0015.mrc,  denoised, 136.1 MB  
        
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        wt75min23030663w185e12u1d05.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.1 GB  
        
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        wt75min34k05_full.rec,  Reconstruction (Pixel Size 1.34 nm), 1.0 GB  
        
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        dwt75min34k05.am,  Segmentation, 2.8 MB  
        
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        dwt75min34k05FL.am,  Segmentation, 2.1 MB  
        
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        dwt75min34k05FL.surf,  Segmentation, 301.7 kB  
        
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        dwt75min34k05IM.am,  Segmentation, 2.8 MB  
        
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        Fig1.tif,  Tif image, 10.3 MB  
        
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        Fig1C.tif,  Tif image, 619.1 kB  
        
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        Fig1_wt.tif,  Tif image, 1.6 MB  
        
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        Fig1_wt1.tif,  Tif image, 5.6 MB  
        
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        Fig1_wtFcolor.tif,  Tif image, 5.2 MB  
        
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        Fig11.tif,  Tif image, 6.1 MB  
        
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        slicer000.tif,  Tif image, 1.6 MB  
        
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        slicer001.tif,  Tif image, 1.1 MB  
        
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        slicer002.tif,  Tif image, 963.2 kB  
        
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        slicer003.tif,  Tif image, 789.1 kB  
        
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        slicer004.tif,  Tif image, 789.1 kB  
        
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        slicer005.tif,  Tif image, 1.1 MB  
        
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        slicer006.tif,  Tif image, 1.3 MB  
        
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        slicer007.tif,  Tif image, 1.3 MB  
        
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        snapshotFtsZMreB.tif,  Tif image, 862.7 kB  
        
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        wt75min34k05stalked.mod,  Segmentation (IMOD), 4.5 MB  
        
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        wt_xy.tif,  Tif image, 1.1 MB  
        
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        wt_xz.tif,  Tif image, 400.3 kB  
        
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        wt_xz1.tif,  Tif image, 1.2 MB  
        
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        wt_yzl.tif,  Tif image, 640.9 kB  
        
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        wt_yzr.tif,  Tif file, 648.8 kB  
        
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        zap000.tif,  Tif file, 1.1 MB  
        
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