DOI: 10.5061/dryad.n5tb2rc6n
title: The PtrRZFP4-PtrJAZ2 module regulates the jasmonic acid signaling pathway involved in the interaction between poplar and herbivorous insects

description: The plant hormone jasmonic acid (JA) is pivotal in regulating plant growth
 and defense mechanisms. Despite extensive research on the JA signaling
 network, the integration of other factors with JAZ (jasmonate ZIM-domain)
 proteins to modulate JA signaling in response to diverse herbivore attacks
 remains unclear. In this study, we employed molecular biology and multi -
 omics techniques to identify an E3 ubiquitin ligase, PtrRZFP4. This E3
 ubiquitin ligase exerts a positive regulatory effect on the JA signaling
 pathway, yet demonstrates differential responses to distinct insect types.
 We found that upon insect attack, the expression of PtrRZFP4 transcripts
 is upregulated. In addition, PtrRZFP4 is able to interact with PtrJAZ2 and
 promote the ubiquitination and degradation of PtrJAZ2 protein.
 Furthermore, PtrRZFP4 activates the jasmonic acid (JA) signaling pathway
 at both the transcriptional and metabolic levels, triggering the synthesis
 of a large number of secondary metabolites related to insect resistance,
 such as terpenoids and alkaloids. This ultimately enhances the
 plant's defense against insect herbivory. The larvae of the
 specialist insect showed a strong preference for feeding on the leaves of
 transgenic poplar overexpressing PtrRZFP4 (OX-PtrRZFP4), which contain
 elevated levels of secondary metabolites. In contrast, the larvae of the
 generalist insect avoid leaves with increased secondary metabolite levels.
 However, PtrJAZ2 exhibits an opposite function to PtrRZFP4 in the
 resistance of poplar trees to different herbivores. Therefore, our study
 uncovers the novel role of the PtrRZFP4-PtrJAZ2 module in plant JA
 signaling and resistance to insect herbivory, highlighting its potential
 for biotechnological applications in improving insect resistance in forest
 trees.
description: # Title of Dataset: The PtrRZFP4-PtrJAZ2 module regulates the jasmonic
 acid signaling pathway involved in the interaction between poplar and
 insects --- Description of the Data and file structure This study uncovers
 the novel role of the PtrRZFP4-PtrJAZ2 module in plant JA signaling and
 resistance to insect herbivory, highlighting its potential for
 biotechnological applications in improving herbivore resistance in forest
 trees. The treatment codes were listed as below: WT-wildtype
 OX-PtrRZFP4-PtrRZFP4 gene overexpressed genotpyes KO-PtrRZFP4-PtrRZFP4
 gene knockout genotpyes Files and variables File: Data1_Herbivore
 bioassays in Fig.1.csv Description: This file contains measurements of
 leaf area consumed (mm²) by larvae of the specialist herbivore Botyodes
 diniasalis and the generalist herbivore Spodoptera frugiperda when feeding
 on different plant genotypes. In addition, the increased fresh weight of
 individual larvae of the specialist herbivore Botyodes diniasalis and the
 generalist herbivore Spodoptera frugiperda fed on leaves of different
 plant genotypes were also recorded. This dataset contains measurements of
 herbivore feeding leaf area and increased fresh weight of the specialist
 and generalist herbivores when they fed on leaves of different poplar
 genotypes Variables leaf feeding area(mm2): the consumed leaf area by
 herbivores increased fresh weight(g): the growth biomass of herbivores fed
 on leaves of different poplar genotypes for seven days File:
 Data2_Immunoblot results in Fig. 5.csv Description: This file contains
 measurements of signal intensity of different poplar genotypes subjected
 to immunoblot Variables signal intensity: signal intensity of different
 poplar genotypes subjected to immunoblot File: Data3_Herbivore bioassays
 in Fig. 6.csv Description: This file contains measurements of leaf area
 consumed (mm²) by larvae of the specialist herbivore Botyodes diniasalis
 and the generalist herbivore Spodoptera frugiperda when feeding on
 different plant genotypes. In addition, the increased fresh weight of
 individual larvae of the specialist herbivore Botyodes diniasalis and the
 generalist herbivore Spodoptera frugiperda fed on leaves of different
 plant genotypes were also recorded. Moreover, the relative expression
 levels of specific genes in poplar leaves over time following different
 applications were detected according to q-RTPCR . Variables leaf feeding
 area(mm2): the consumed leaf area by herbivores increased fresh weight(g):
 the growth of herbivores fed on leaves of different poplar genotypes
 Relative expression level: relative expression levels of specific gene
 over time following different applications File: Data4_Field experiment in
 Fig. 7.csv Description: This file contains measurements of growth-related
 traits of different poplar genotypes grown in the field. Variables basal
 stem diameter(mm): stem growth of different poplar genotypes plant height
 (cm): growth height of different poplar genotypes leaf water content %:
 leaf water content of different poplar genotypes leaf fresh weight (g):
 leaf biomass of different poplar genotypes stem fresh weight (g): stem
 biomass of different poplar genotypes pest index: herbivore damage on the
 leaves of different poplar genotypes File: File: Data5_Supplementary
 Figures.csv Description: This file contains measurements of relative gene
 expression levels of different poplar genotypes Variables relative
 expression level: relative gene expression level of different poplar
 genotypes in the q-rtPCR analysis ## Sharing/access Information Links to
 other publicly accessible locations of the data: N.A. Was data derived
 from another source? No