cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2012.265
title: Fabrication of anion-selective electrodes from anthracene-based and calix[4] arene-based copper (II) comlexes containing tripodal amine pendants

description: Three types of ionophores were designed and synthesized with different number of side-arms, linkers and building blocks, and the effects of their structures on anion sensing properties were then comparatively examined. Ionophore I was synthesized in 73% yield by attaching tripodal amine mononuclear Cu(II) complex directly to the anthracene, while two units of such complex were connected to the p-tert-butylcalix[4]arene with 2-alkoxybenzyl and amide linkers to give ionophores II and III in 42 and 58% yield, respectively. The polymeric membrane sensors containing these ionophores were fabricated and then the sensitivity, selectivity, optimal membrane composition, as well as the important characteristics of electrodes were then explored by varying types and amounts of ion exchangers. The potentiometric investigations indicated that ionophore I could not function properly as an anion carrier due to its low lipophilic single-sided arm structure in membrane electrodes. On the other hand, ionophores II and III exhibited good sensitivity and selectivity toward iodide even in the absence of ion exchangers, resulting from the dinuclear Cu(II) complexes as an effective ion-carrier unit, providing a suitable cavity for iodide. The best iodide-selective electrode was achieved when using ionophore II (10 mmol kg-1) in the presence of 25 mol% of tridodecylmethylammonium chloride (TDMACl) anion exchanger related to the ionophore incorporated in the o-NPOE plasticized PVC membrane, with the Nernstian slope of -54.9 ± 0.2 mV decade-1 of iodide response, the linear working range from 10-5 to 10-2 M, the fast response time less than 5 s, and the limit of detection of 3.3  10-6 M. UV-Visible spectrophotometric studies were also carried out and the spectral shift was observed to affirm the interaction between ionophore and iodide. Moreover, this electrode could be used over a wide pH range of 4.7-8.6 with great reversibility, and also applied to determine the concentration of iodide in the real water samples, mineral water and tap water, with satisfactory recoveries.
DOI: 10.14457/cu.the.2012.266
title: Stereoscopic face reconstruction from a single 2D face image using orthogonality of normal surface and Y-Ratio

description: This research proposes a 3D face reconstruction method with single image and condition of Lambertian model. This method can be divided into 4 parts: surface albedo estimation, normal surface calculation, actual height calculation and accuracy evaluation. The first part which is surface albedo estimation started with initial normal surface from Average face data and morphed it to match an input image by my pattern morphing method. Then the estimated normal surface, Intensity map and Light source vector of input image are used to estimate the albedo of each point on input image. The second part, normal surface calculation, started with finding the relation equation of Normal vector in X and Y axis and calculate the normal surface. The third part is pixels’ height calculation. And the last part is a accuracy evaluation of the method and surface display. The experiment was done with 114 face’s images from 2 face’s database compared with the minimization approach and the result shown that my approach gave the better accuracy and consumed the less process time.
DOI: 10.14457/cu.the.2012.267
title: Protective effect of plaunotol in doxorubicin-induced apoptosis in human renal HK-2 cells through antioxidant mechanisms

description: In searching for the safe and effective compounds to be used as chemoprotective agents to cease toxicity of doxorubicin on renal cells, plaunotol which is a major constituent in Croton stellatopilosus Ohba extract was studied for its possible effect. The results showed that plaunotol exhibited the protection against doxorubicin-induced cell death in human proximal tubule cells. Treatment of the renal cells with doxorubicin resulted in the significant decrease of cell viability, mainly due to the cause of apoptosis. Pretreatment of the cells with plaunotol for at least 9 h prior doxorubicin exposure rendered cell survival against the toxicity of doxorubicin. In terms of mechanism, plaunotol was shown to up-regulate the level of anti-apoptotic Mcl-1. It was clearly showed that the decrease of Mcl-1 level after doxorubicin treatment was closely associated with the toxic action of the drug, and the pretreatment of plaunotol caused the increase of Mcl-1 was able to prevent the doxorubicin-induced cell death. Furthermore, it was found that the lowering of Mcl-1 was closely related to the presence of reactive oxygen species (ROS). Pretreatment of an antioxidant and plaunotol appeared to retrieve the Mcl-1 level from ROS-induced diminution. Antioxidant activity of plaunotol in human renal cells was clearly observed after the incubation with plaunotol for 3 h. The reduction of ROS in the cells appeared to come from the increase of cellular glutathione which was induced by plaunotol treatment. These results suggest that the nephroprotective effect of plaunotol is a result from its antioxidant activity which in turn preserves the level of anti-apoptotic Mcl-1 protein.
DOI: 10.14457/cu.the.2012.268
title: Role of lactobacillus thai strains in anti-inflammatory and antagonistic activity to helicobacter pylori

description: Helicobacter pylori is the causative agent of gastric-associated disease such as gastritis, peptic ulcer, and gastric cancer. To search for probiotic bacteria with the potential adjuncts in the treatment of H. pylori-associated diseases, human gastric-derived Lactobacillus spp. were tested for the two main activities, anti-inflammatory and antagonistic activity against H. pylori. For anti-inflammatory activity, Lactobacillus conditioned media (LCM) of eight isolates showed the suppressive effect to IL-8 production which was not related to inhibition of H. pylori proliferation. LCM of L. salivarius B101 (LS-B101), L. rhamnosus B103 (LR-B103), and L. plantarum XB7 (LP-XB7) had suppressive effect to IL-8 mRNA expression in H. pylori-stimulated AGS cells by contributing to the inhibition of NF-κB and with the addition of c-Jun inactivation for LP-XB7. The anti-inflammatory effect of LP-XB7 was further assessed in vivo using a H. pylori-infected Sprague-Dawley rat model. Strain LP-XB7 contributed to a delay in the detection and colonization of H. pylori in rat stomachs, attenuated gastric inflammation, and ameliorated gastric histopathology. Additionally, the administration of LP-XB7 correlated with the suppression of TNF-α and CINC-1 in sera, and suppression of CINC-1 in the gastric mucosa of H. pylori-infected rats. These results suggest that LP- XB7 produces secreted factors capable of modulating inflammation during H. pylori infection. For antagonistic activity, screening of eighty-five isolates revealed that L. salivarius B23 (LS-B23) and B37 (LS-B37) showed positive result to H. pylori reference strains by spot-overlay method. LS-B37 was chosen for further characterization due to its strongest inhibition. Secreted antagonistic substance of LS-B37 was found to be, acid-alkali tolerant, heat-stable, stable over 3 months, not related to lactic acid, and approximately 3-10 kDa. LS-B37 also inhibited the growth of clarithromycin- and metronidazole-resistant H. pylori clinical isolates. Therefore, L. plantarum (LP-XB7) and L. salivarius (LS-B37) show promise as an adjunctive therapy for treating H. pylori-associated disease.
DOI: 10.14457/cu.the.2012.269
title: Development of 3D-fibrous structure and fluffy yarn via dual-polarity co-electrospinning process

description: In this research we had developed a novel electrospinning process, a dual polarity co-electrospinning, that could be used to produce 3 dimensional structure.The research was separated into 3 main parts i.e. a) an investigation of processing parameters effect on formation and morphology of poly (caprolactone) (PCL) electrospun fibers, b) a development of dual polarity co-electrospinning process and c) a study on the effect of fiber size and fiber mat structureon cell matrix interaction. In the first part, the effect of PCL solution concentration, applied voltage, collecting distance and solution flow rate on PCL fiber morphology were assessed. In the second part, the dual polarity co-electrospinning process were developed. This technique utilized a positive and negative charge simultaneously. Under certain spinning condition, a 3 dimension structures from PCL electrospun fiber in the 3D-fibrous and fluffy yarn structure were formed. The effect of the applied opposite charge, flow rate, fiber size, ratio of fiber and spinning rate of the collectoron fiber formation were studied. The morphologies and porosity (distribution of porosity and pore size) of a 3D-fibrous and fluffy yarn structure of PCL were determined using scanning electron microscope (SEM) and porosimeter. The 3D-fibrous and fluffy yarn had more porosity compared to fiber mat from the ordinary electrospinning process.In the last part, PCL fiber mats with three different fiber sizes, PCL fiber in the form of 3D-fibrous structure and fluffy yarns were prepared and used to evaluate effects of fiber size and fiber structure on cell adhesion and proliferation using L929 as a model cell. The results showed that both size and structure played a role in prohibiting or accommodating cellular distribution or penetration into the under layer of electrospun fiber mat.
DOI: 10.14457/cu.the.2012.270
title: Evaluation for obstructive sleep apnea using CBCT parameters and sleep questionnaire in a group of Thai population

description: Objectives : To compare parameters of upper airway dimension from upright CBCT between obstructive sleep apnea (OSA) and habitual snorers as well as to investigate the relationship of CBCT values combined with STOP-BANG questionnaire. Methods : Forty five samples, consisting of 14 habitual snorers (AHI &lt; 5 events/hour) and 31 consecutive OSA patients (AHI ≥ 15 events/hour), were recruited for this study. Volumetric data from an upright CBCT unit (3D Accuitomo 170, J.Morita) with 140x100 mm field of view were obtained; meanwhile, STOP-BANG questionnaire were completed. Upper airway dimensions from CBCT data were measured using InVivo 5.1 software by one investigator twice within 1-week interval. All data were then statistically analyzed using SPSS software. Results : There were statistically significant differences among habitual snorers and OSA subjects in terms of CBCT parameters including volume, minimum cross-sectional area, average cross-sectional area, anteroposterior and lateral dimensions, and uniformity of the airway and soft palate length. For the STOP-BANG questionnaire, OSA subjects were observed to be older, being male, having more loudly snoring, and having observed apnea. Conclusions : Volume, minimum cross-sectional area, average cross-sectional area, anteroposterior and lateral dimensions, and uniformity of the airway and soft palate length were statistically significant different between habitual snorers and OSA subjects. These parameters could be of use to develop a prediction model in a group of Thai population.
DOI: 10.14457/cu.the.2012.271
title: Genetic and pathogenic characterization of thai swine influenza viruses after the introduction of pandemic H1N1 2009

description: Since pandemic H1N1 (pH1N1) 2009 influenza virus emerged in April 2009, at least 18,449 people died and was called Swine-origin 2009 A (H1N1) or “swine flu” due to its genetic character. Swine influenza virus (SIV) surveillance was conducted from June 2010 to May 2012 in Thailand. The results of the surveillance revealed that pH1N1 was the most prevalence in 2010. Later, reassortant H1N1, H3N2 and H1N2 viruses were also isolated. As a result of the discovery of the emergent SIV, pathogenesis studies of this novel virus was conducted in order to plan for future disease protection and control measures both in swine and human populations. Hence, pathogenesis studies in pigs and commingling sentinel ducks were conducted. The pH1N1 and its reassortant virus (rH1N1) isolated from pigs in Thailand were inoculated into 2 separate cohorts. A day later, sentinel ducks were commingled with pH1N1 or rH1N1 inoculated pigs in separate groups. This present studies suggested that both pH1N1 and rH1N1 viruses were able to infect and replicate in pigs and sentinel ducks. Moreover, those viruses were capable to transmit from infected pigs to sentinel ducks when animals were commingling but viral replication in ducks was limited. From the study, virological and serological surveillance of swine influenza are of importance for the prevention and control of swine influenza and for preparedness of future pandemic influenza in humans.
DOI: 10.14457/cu.the.2012.272
title: The microbiota associating with three different transmucosal implant designs

description: Objective To investigate the putative pathogenic bacteria, Porphyromonas gingivalis (Pg), Treponema denticola (Td), or Tanerella forsythia (Tf ), associating with implant-supported dental prostheses using differential transmucosal designs. Methodology Forty-five partially edentulous patients who received implant-supported fixed partial prostheses were randomly selected and categorized into three groups based on the transmucosal implant-abutment connections, including the platform-matching abutments (n = 15), platform-switching abutments (n = 15), and tissue-level dental implants (n = 15). To obtain subgingival bacterial samples, four paper points were inserted under light pressure in healthy peri-implant sulci, and DNA was extracted from the samples. Pg-, Td-, or Tf -specific DNA was detected by endpoint PCR. Results There was no statistically significant differences (P &gt; 0.05) in the frequencies of the Pg, Tf, or Td associating with differential transmucosal designs under healthy conditions. However, the proportion of Tf associating with platform-matching abutments or platform-switching abutments was substantially higher than Pg or Td. The chi-square test showed significant differences (P &lt; 0.05) in the frequencies of these bacteria in the groups restored with platform-matching abutments or platform-switching abutments, but not tissue-level dental implants. Conclusion periodontal pathogens are part of the normal resident microbiota and may be found at healthy peri-implant sites.
DOI: 10.14457/cu.the.2012.273
title: Sample preparation by dispersive liquid-liquid microextraction for determination of pesticide residues in ginger

description: In this thesis, the multiresidues of organophosphates (OPPs), organo- chlorines (OCLs) and pyrethoids (PYs) pesticides in ginger were determined. The OPPs, OCLs and PYs pesticides in ginger sample were extracted by liquid-liquid extraction with acetonitrile combined with dispersive liquid-liquid microextraction (DLLME) and analyzed by gas chromatography–mass spectrometry. Various parameters that affected the extraction efficiency of DLLME including type and volume of extraction and disperser solvents, extraction time, centrifugation time, salt addition, and pH were evaluated. The optimum conditions were using 50 μL of tetrachloroethylene as the extraction solvent, 1 mL of acetonitrile as the disperser solvent, extraction time of 2 min, centrifugation time of 5 min, and the addition of 4% of sodium chloride. Recovery tests were performed at concentrations levels of 5.0, 15.0 and 100.0 ng/g; recoveries for each target were in the range between 60 to 105%. Limits of detection and limits of quantitation of this method were ranging from 2.2 to 3.1 ng/g and 7.2 to 10.2 ng/g, respectively. The repeatability of the proposed method, expressed as HORRAT, was in range from 0.13 to 1.86. Compared with the conventional sample preparation method, the proposed method has the advantage of being quick and easy of operation, and low consumption of organic solvent.
DOI: 10.14457/cu.the.2012.274
title: Time-temperature paper indicator from polydiacetylene

description: The polydiacetylene-based (PDA-based) time-temperature indicators (TTIs) are successfully prepared from commercially available diacetylene (DA) monomers i.e. 10, 12-pentacosadiynoic acid (PCDA) and 10, 12-tricosadiynoic acid (TCDA). The indicators are fabricated on filter paper strips by dip-coating technique followed by UV irradiation at 254 nm under controlled temperature at 278 ± 1 K. The color transition of the indicators at 300 ± 1 K is monitored by a webcam. The color images are converted to RGB values and the color transition time is determined by intersection point of %R and %B curves in their plots against time. The blue PDAs themselves do not change their color at room temperature. In the presence of 1-dodecanol, the blue-to-red color transition at the tested temperature is observed without time dependence. With the addition of cationic surfactant such as DTAB, TTAB or HTAB, the DA/1-dodecanol/surfactant three-component TTIs show desirable time dependent color transition. The color transition time can be tuned in the range of 1.5-6.5 days by the amount and selection of the cationic surfactant.
DOI: 10.14457/cu.the.2012.275
title: Isolation of endophytic Xylaria spp. from Trad province and their metabolites with anti-cervical cancer activity

description: Metabolites from microorganisms are considered to be a rich source of novel lead compounds for drug development. Endophytic fungi are increasingly used to screen for such compounds because they are relatively unexplored until now. In this study, we isolated endophytic Xylaria spp. from plant leaves sampling in Trad Province in eastern Thailand, and screened for biological activity of the isolated fungal metabolites. A total of 83 isolates included 41 Xylaria spp., 5 Daldinia spp., 1 Fusarium spp., 1 Collectotrichum spp., 4 Pestalotiopsis spp., 2 Phomopsis spp., 2 Phyllosticta spp., 1 Trichoderma spp., 4 Nigrospora spp. and 22 Mycelia sterilia. Crude extracts with ethyl acetate of all endophytic Xylaria spp. were screened for antimicrobial activity against 5 reference pathogenic bacteria and yeast. Moreover, nine crude extracts with strong antimicrobial activity were tested for cytotoxicity against human papiloma virus positive cervical cancer cell line, CaSki. Among these isolates, endophytic fungi isolate X-17 showed both strong antimicrobial and anticancer activity. Crude extract from X-17 showed cytotoxic activity against human cancer cell line, with the inhibitory concentration 50 (IC50) of 10.05±0.28 µg/ml. Detailed study on the cytotoxicity of the X-17 against CaSki cell line demonstrated that this extract induced cell cycle arrest and apoptosis. Therefore, it was chosen for further test for anti-inflammatory activity. Nevertheless, crude extract from X-17 did not show anti-inflammatory activity. Purification of bioactive compounds from this extract was carried out by liquid chromatography and two pure compounds were obtained that were identified as cytochalasin D and 2-p-tolylethanol. In addition, morphology, cultural characteristics and ITS (internal transcribe spacer) sequencing analysis of endophytic isolate X-17 confirmed that it belongs to Xylaria laevis.
DOI: 10.14457/cu.the.2012.276
title: Improving cold flow property of biodiesel from neem seed oil with esters of palm fatty acid distillate

description: The research aimed to improve the cold flow property of biodiesel from neem oil (NME) by using 14 types of synthesized pour point depressants (PPDs) which were isopropyl ester (IPE), 2-butyl ester (BE), methyl laurate (ML), methyl palmitate (MP), methyl oleate (MO), methyl linoleate (MLi), isopropyl laurate (IPL), isopropyl palmitate (IPP), isopropyl oleate (IPO), isopropyl linoleate (IPLi), 2-butyl laurate (BL), 2-butyl palmitate (BP), 2-butyl oleate (BO), and 2-butyl linoleate (BLi). All of these PPDs were synthesized by esterification reaction and characterized by Nuclear magnetic resonance spectroscopy (1H NMR). The results indicated that cloud point (CP) and pour point (PP) of biodiesel from neem oil (NME) could be decreased in the range of 1.0±1.0 °C to 7.0±1.0 °C and from 1.0±1.0 °C to 6.0±1.0 °C, respectively. BL gave the best result on improving cold flow property of biodiesel due to the steric hindrance of branched chain alcohol. At 300,000 ppm of BL, cloud point (CP) and pour point (PP) of NME were decreased from 15.0±1.0 °C to 8.0±1.0 °C and from 10.0±1.0 °C to 4.0±1.0 °C, respectively.
DOI: 10.14457/cu.the.2012.277
title: Transport of macromolecules in glomerular filtration

description: The main function of kidneys is to retain the normal blood composition and volume through blood filtration: the first step of which occurs at the glomerular capillary wall that consists of three layers: the endothelium cell layer, the glomerular basement membrane (GBM) and the epithelial foot processes with their interconnecting slit diaphragm. Our research focuses on the transport of myoglobin, a biomarker for muscle damage and one of the causes of acute renal failure. A hydrodynamic model is introduced to describe hindered transport of electrically-neutral macromolecules through the epithelial slit and a glomerular basement membrane (GBM). The slit diaphragm is modeled as a row of parallel cylindrical fibers and the GBM is treated as an isotropic fibrous medium. Numerically calculated sieving coefficient of myoglobin, a product of the sieving coefficient of the slit diaphragm and that of the GBM, is found to be in good agreement with sieving coefficient obtained from counting radioactivity of radiolabeled myoglobins in blood and urine, if, in the calculation, GBM fiber radii are assumed to be 0.5 nm (with the presence of type IV collagen in the GBM neglected). With the gap between adjacent fibers in the epithelial slit diaphragm (L) assumed to be about 10 nm, the absence of the slit diaphragm increases the sieving coefficient by only 6-7%. It seems that the GBM contributes significantly to the restriction of myoglobin in glomerular filtration, although it might still be too early to say that the contribution of slit diaphragm is negligible, given that the values of L = 10 nm is the upper limit. Further investigation is needed to fully understand the contribution of each layer.
DOI: 10.26009/s0fcqnqr
title: TDWB_17_1

description: Experiment to explore shelf-edge deltas and coupled downslope submarine fan systems that experience changes in relative sea level. Stage 1 included construction of system with constant relative sea level, stage 2 captured system evolving under short relative sea level cycles with small magnitudes, stage 3 captured system evolving under a long relative sea level cycles with a large magnitude. Size and durations of relative sea level cycles were scaled to autogenic scales. Input flow was delivered with a flood cycle and input flow contained dissolved salt to promote plunging of turbidity currents at shelf-edge.
DOI: 10.5281/zenodo.3372822
title: Data for: Soil domestication by rice cultivation results in plant-soil feedback through shifts in soil microbiota

description: This repository contains the data for the manuscript entitled "Soil domestication by rice cultivation results in plant-soil feedback through shifts in soil microbiota." Analysis scripts can be found at https://github.com/bulksoil/SoilDomestication
DOI: 10.5281/zenodo.3377709
title: TESS-APRIL-2019

description: Measurements taken by the European Photometer Network (Project STARS4ALL). April 2019
DOI: 10.5281/zenodo.3377710
title: TESS-APRIL-2019

description: Measurements taken by the European Photometer Network (Project STARS4ALL). April 2019