DOI: 10.5061/dryad.fttdz08rb
title: Oxygen consumption of juvenile brown trout, Salmo trutta, under varying thermal conditions during embryogenesis

description: Climate change is predicted to increase the future thermal conditions in
 northern latitudes with the potential effect of altering the metabolic
 scope and potential fitness of aquatic ectotherms. We experimentally
 tested the effect of elevated egg incubation temperature on the metabolic
 scope in juvenile brown trout (Salmo trutta). Brown trout cohorts from
 anadromous and resident crosses were raised from egg through exogenous
 feeding of juveniles in either natural river temperatures (cold) or
 elevated (+ 3 °C, warm) temperatures. In respirometry studies, we measured
 oxygen consumption rates of juvenile trout from both incubation
 temperatures and all possible breeding crosses after they were feeding
 exogenously and at an ambient temperature of 13 °C. These measures were
 taken over a period where the trout were resting, allowing for the
 determination of standard metabolic rate (SMR), and followed by a chase to
 exhaustion allowing for the measure of the maximum metabolic rate (MMR).
  The aerobic scope (AS) of these juveniles from four
 anadromous-resident crosses and from both incubation temperatures could
 then be calculated as AS = MMR – SMR. This dataset represents a key to all
 respiration trials including: fish embryonic incubation temperature,
 parental cross, fish total length (mm), calculated mass (g) per fish,
 ventilation rate (opercular beats/min), and water temperature per
 respiration trial. For each fish tested, the dissolved oxygen levels in
 the respirometry chamber were recorded continuously through periods of
 static respiration and recharge flow in 15 sec intervals for the duration
 of the trial testing periods (~ 5-7 hrs).  These data were
 evaluated for SMR and MMR using a respiration program (respR) in the R
 statistical program.
description: Respiration (oxygen consumption) and ventilation rates were
 measured on juvenile trout from two incubation temperature and four
 parental crosses from June 1 to 4 June, 2018. Two static flow respirometry
 systems, each with four fiber-optic dissolved oxygen sensors per system
 (two 4-channel FireStingO<sub>2</sub> Optical Oxygen Meters,
 PyroScience™, Aachen,
 Germany) measured dissolved oxygen in 50 ml respirometry
 chambers individually housing trout. An external digital thermocouple
 (Pt100-TSUB21 Temperature Probe, PyroScience™, Aachen, Germany) from
 each system enabled automatic temperature compensation of the oxygen
 sensor signals by the FireStingO<sub>2</sub> software. A
 single 500 L fiberglass tank (water depth 30 cm), continuously supplied
 both submerged systems with fresh river water. With two systems of four
 optical sensors each, all eight parental cross and incubation temperature
 could be measured simultaneously. Measurement intervals were 15 s per
 reading with 15 min under static flow (i.e. no flow), and 15 min as a
 water recharge period over a 5-7 hr trial. The 50 ml size of each chambers
 did not facilitate mixing devices, but opercular ventilation and fish
 movements contributed to mixing in the chambers. Baseline measures of
 dissolved oxygen were taken at the initial and conclusion of the trials.
 During the last static respiration measurement period, a video recording
 was made of each fish so their ventilation rates in opercular beats per
 min could be determined. At the conclusion of measurements on resting
 trout, each fish was chased to exhaustion (2 min) in a circular chase
 chamber and quickly (&lt; 10 sec) returned to the respirometry chamber
 for measures of maximum oxygen consumption. All fish were only tested
 once, and if trials had oxygen levels &lt; 80% of recharge levels
 after three measurement periods these fish were omitted (n = 8).  In this
 dataset, those trials include trout #: 2, 30, 51, 54, 55, 67, 78, and 79.
 While 12 replicate trials were started, 11 were used because of a computer
 malfunction at the initiation of data recording in trial 4.
description: This is an Excel file with the initial tab including a key and
 summary data on the fish and respirometer conditions for each trial. The
 remaining 88 worksheet tabs contain the dissolved oxygen measures for each
 individual fish tested. Each measure represents an interval of 15 sec. The
 data (8 fish: #2, 30, 51, 54, 55, 67, 78, and 79) that did not meet the
 criteria of oxygen levels falling below 80% of the recharge oxygen level
 past the first three measures are marked on their data
 worksheets.