DOI: 10.5061/dryad.4mw6m90pq
title: Data from: Longer matings increase male competitive fertilization success in <em>Drosophila melanogaster</em>

description: Copulation duration is a variable behavior that is often extended by males
 beyond the time needed for sperm transfer. In many insect species, these
 prolonged matings have been linked to higher male reproductive success
 with virgin females. However, most matings in multiply-mating species
 involve non-virgin females, whose role in shaping the evolution of this
 behavior remains underexplored. Using hemiclonal analysis in Drosophila
 melanogaster, we tested whether males benefit from extended matings with
 non-virgin females by comparing competitive fertilization success (P2)
 between males from three long-mating and three short-mating hemiclone
 lines. We confirmed that differences among hemiclone lines in male mating
 duration with virgin females persisted with non-virgin females and that
 these longer matings were associated with higher P2, demonstrating a clear
 fitness benefit to males. These findings build on past work showing that
 longer matings increase male defensive reproductive success, highlighting
 the adaptive value of prolonged matings for males in multiply-mating
 species and underscoring the importance of including non-virgin females in
 studies of sexual selection and reproductive behavior.
description: # Data from: Longer matings increase male competitive fertilization
 success in *Drosophila melanogaster* ## Description of the data and file
 structure **HCMDP2_MV.csv** *Mating duration data from the mating duration
 experiment with virgin and non-virgin large and small females. Data are
 only shown for hemiclone males that mated. Data are displayed in Figure
 1.* “Block” = the experimental block “Observer” = the observer “Vial” =
 the vial label for that block "Clone" = the specific hemiclone
 line “Treatment” = the mating duration treatment (Long or Short) for that
 hemiclone line “FemSize” = the size of the female the male was paired with
 ("L" = large, "S" = small) "FemStatus" = the
 mating status of the female the male was paired with (M= mated/non-virgin,
 V = virgin) "CloneDuration" = the mating duration for the
 hemiclone male in minutes. **HCMDP2_Remate.csv** *Female remating data
 from the male offensive reproductive success experiment.* “Block” = the
 experimental block “Observer” = the observer “Vial” = the vial label for
 that block "Clone" = the specific hemiclone line “FemSize” = the
 size of the female the male was paired with ("L" = large,
 "S" = small) “Treatment” = the mating duration treatment (Long
 or Short) for that hemiclone line "Mated" = whether or not the
 hemiclone male mated with the non-virgin female he was paired with (1=
 Mated, 0 = did not mate) **HCMDP2.csv** *Mating duration data and P2 data
 from the male offensive reproductive success experiment. Data are only
 shown for hemiclone males that mated with the non-virgin female. Data are
 displayed in Figures 2a and 2b.* “Block” = the experimental block
 "Observer" = the observer of the hemiclone mating (only used for
 the mating duration analysis) “Vial” = the vial label for that block
 "Clone" = the specific hemiclone line “FemSize” = the size of
 the female the male was paired with ("L" = large, "S"
 = small) “Treatment” = the mating duration treatment (Long or Short) for
 that hemiclone line "CloneDuration" = the mating duration for
 the hemiclone male in minutes. Empty cells represent instances in which
 the mating duration could not be determined (because either the start time
 or end time of the mating was missed) "RedOff" = the number of
 red-eyed offspring produced by that female (i.e., sired by the hemiclone
 male) "BwOff" = the number of brown-eyed offspring produced by
 that female (i.e., not sired by the hemiclone male) "TotalOff" =
 the total number of offspring produced by that female "OLRE" =
 an observation-level random effect added to control for overdispersion in
 the P2 analysis ## Sharing/Access information Links to other publicly
 accessible locations of the data: N/A Data was derived from the following
 sources: N/A ## Code/Software ##### R scripts for models: All analyses
 performed using R 4.5.1 and the following packages: glmmTMB 1.1.11 car
 3.1.3 emmeans 1.11.1 DHARMa 0.4.7 **HCMDP2_MV.R** R code for the linear
 mixed model testing for differences between male treatments in mating
 duration with virgin and non-virgin large and small females using the data
 in HCMDP2_MV.csv **HCMDP2_Remate.R** R code for the binomial GLMM testing
 for differences between male treatments in female remating rates using the
 data in HCMDP2_Remate.csv **HCMD_P2.R** R code for the following analyses
 using the data in HCMDP2.csv: * the linear mixed model testing for
 differences between male treatments in mating duration with non-virgin
 large and small females * the binomial GLMM testing for differences
 between male treatments in P2 with large and small females