cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.25549551.v1
title: Rethinking the relationship between desiccation-tolerant vascular plants and water deficit

description: Water deficit is one of the main drivers of plant mortality and is projected to be more critical owing to climate change. Because desiccation-tolerant vascular plants (DT plants) can cope with water deficit, the paradigm emerges of their strong association with and restricted occurrence to locations characterised by water deficit conditions. However, this paradigm is not supported by earlier studies, hampering our understanding of the species – environment relationships and the vulnerability of DT plants to climate change. We tested this paradigm and provide an evaluation of the vulnerability of DT plants to climate change. We estimated the diversity and distribution of DT plants along water deficit gradients and assessed species vulnerability to climate change from a climatic perspective and over broad phylogenetic and macroecological scales. The diversity and distribution of DT plants were neither associated with, nor restricted to, locations characterised by water deficits. Species with narrow niche breadth and poikilochlorophylly might be the most vulnerable to climate change. Our findings suggest that the desiccation events DT plants undergo are promoted by topo-edaphic conditions rather than by climate. We suggest that ecologically restricted species and those with a poikilochlorophyllous strategy should be prioritised for conservation.
DOI: 10.6084/m9.figshare.25549705
title: Occurrence of preanthesis cleistogamy in <i>Richardia brasiliensis</i> suggests it may be more common in Rubiaceae than realised

description: Most Rubiaceae species are hermaphroditic with outcrossing often promoted by distyly and self-incompatibility, as well as by andromonoecy and dioecy in some species. However, cleistogamy has been reported in three genera, including <i>Richardia</i>. To characterise the reproductive biology of <i>Richardia brasiliensis</i> (Rubiaceae), a ruderal herb native to southern USA, Central America and large parts of South America, and determine if it exhibits preanthesis cleistogamy. In three natural populations of <i>R. brasiliensis</i> in Argentina, floral processes were monitored in the flower-bud stage and during anthesis until senescence. In addition, stigma receptivity and pollen viability were determined at these different floral stages in two populations, and experimental controlled pollinations were performed to check for spontaneous self-pollination, natural pollination and apomixis. Floral visiting insects were observed and collected for identification. Autonomous self-pollination occurred when flowers were in bud and stigmas were receptive, with ovules fertilised prior to anthesis. These flowers were protogynous and functionally cleistogamous, though later passed through a chasmogamous phase when flowers opened and were visited by diverse insects. Our study demonstrates that preanthesis cleistogamy occurs in flower-buds of <i>R. brasiliensis</i>, indicating that this mode of self-pollination and self-fertilisation may be more common in Rubiaceae than previously realised. Flowers of <i>Richardia brasiliensis</i> exhibit two striking features in the flower-bud stage: stigmatic receptivity and self-pollination causing preanthesis cleistogamy to occur.Pollen grain germination on distinctly enlarged papillae cells and pollen tube growth in the style to the ovary were observed in 85% of fixed flower-buds.During anthesis, flowers are visited by a diversity of insects collecting pollen and nectar (Orders Hymenoptera (Apidae, Formicidae and Trigonalidae) and Diptera (Syrphidae)), but due to preanthesis cleistogamy most reproduction occurs via self-fertilisation. Flowers of <i>Richardia brasiliensis</i> exhibit two striking features in the flower-bud stage: stigmatic receptivity and self-pollination causing preanthesis cleistogamy to occur. Pollen grain germination on distinctly enlarged papillae cells and pollen tube growth in the style to the ovary were observed in 85% of fixed flower-buds. During anthesis, flowers are visited by a diversity of insects collecting pollen and nectar (Orders Hymenoptera (Apidae, Formicidae and Trigonalidae) and Diptera (Syrphidae)), but due to preanthesis cleistogamy most reproduction occurs via self-fertilisation.
DOI: 10.6084/m9.figshare.25549705.v2
title: Occurrence of preanthesis cleistogamy in <i>Richardia brasiliensis</i> suggests it may be more common in Rubiaceae than realised

description: Most Rubiaceae species are hermaphroditic with outcrossing often promoted by distyly and self-incompatibility, as well as by andromonoecy and dioecy in some species. However, cleistogamy has been reported in three genera, including <i>Richardia</i>. To characterise the reproductive biology of <i>Richardia brasiliensis</i> (Rubiaceae), a ruderal herb native to southern USA, Central America and large parts of South America, and determine if it exhibits preanthesis cleistogamy. In three natural populations of <i>R. brasiliensis</i> in Argentina, floral processes were monitored in the flower-bud stage and during anthesis until senescence. In addition, stigma receptivity and pollen viability were determined at these different floral stages in two populations, and experimental controlled pollinations were performed to check for spontaneous self-pollination, natural pollination and apomixis. Floral visiting insects were observed and collected for identification. Autonomous self-pollination occurred when flowers were in bud and stigmas were receptive, with ovules fertilised prior to anthesis. These flowers were protogynous and functionally cleistogamous, though later passed through a chasmogamous phase when flowers opened and were visited by diverse insects. Our study demonstrates that preanthesis cleistogamy occurs in flower-buds of <i>R. brasiliensis</i>, indicating that this mode of self-pollination and self-fertilisation may be more common in Rubiaceae than previously realised. Flowers of <i>Richardia brasiliensis</i> exhibit two striking features in the flower-bud stage: stigmatic receptivity and self-pollination causing preanthesis cleistogamy to occur.Pollen grain germination on distinctly enlarged papillae cells and pollen tube growth in the style to the ovary were observed in 85% of fixed flower-buds.During anthesis, flowers are visited by a diversity of insects collecting pollen and nectar (Orders Hymenoptera (Apidae, Formicidae and Trigonalidae) and Diptera (Syrphidae)), but due to preanthesis cleistogamy most reproduction occurs via self-fertilisation. Flowers of <i>Richardia brasiliensis</i> exhibit two striking features in the flower-bud stage: stigmatic receptivity and self-pollination causing preanthesis cleistogamy to occur. Pollen grain germination on distinctly enlarged papillae cells and pollen tube growth in the style to the ovary were observed in 85% of fixed flower-buds. During anthesis, flowers are visited by a diversity of insects collecting pollen and nectar (Orders Hymenoptera (Apidae, Formicidae and Trigonalidae) and Diptera (Syrphidae)), but due to preanthesis cleistogamy most reproduction occurs via self-fertilisation.
DOI: 10.6084/m9.figshare.25040906
title: Genetic polymorphism analysis and forensic application evaluation of 57 insertion/deletion polymorphisms from Yi ethnic group in Yunnan

description: As a new kind of diallelic genetic marker, insertion/deletion (InDel) polymorphisms have recently been used in forensic science. However, there are relatively few studies on the forensic evaluation of InDel genetic polymorphisms from different populations. The aim of the present work is to assess the genetic polymorphism and forensic applicability of 57 InDels from the Yi ethnic group and explore the genetic background of this group. A total sample of 122 unrelated individuals of Yi group from the Yunnan province were genotyped by the AGCU indel 60 Kit. Multiplex population genetic analyses on the same 57 InDels were carried out among the Yunnan Yi group and 29 reference populations. The average allele frequency of these loci in the Yi ethnic group was 0.485. Heterozygosity, polymorphism information content, and the power of discrimination were 0.477, 0.362, and 0.612, respectively. The combined power of discrimination and the combined power of exclusion reached to 0.99999999999999999669 and 0.999962965, respectively. The results showed that 57 InDels polymorphisms have high genetic polymorphisms in the Yi ethnic group. The 57 InDels could be used for forensic individual identification, paternity testing, and intercontinental population discrimination, with the potential for use in biogeographic ancestry inference.
DOI: 10.6084/m9.figshare.25040906.v1
title: Genetic polymorphism analysis and forensic application evaluation of 57 insertion/deletion polymorphisms from Yi ethnic group in Yunnan

description: As a new kind of diallelic genetic marker, insertion/deletion (InDel) polymorphisms have recently been used in forensic science. However, there are relatively few studies on the forensic evaluation of InDel genetic polymorphisms from different populations. The aim of the present work is to assess the genetic polymorphism and forensic applicability of 57 InDels from the Yi ethnic group and explore the genetic background of this group. A total sample of 122 unrelated individuals of Yi group from the Yunnan province were genotyped by the AGCU indel 60 Kit. Multiplex population genetic analyses on the same 57 InDels were carried out among the Yunnan Yi group and 29 reference populations. The average allele frequency of these loci in the Yi ethnic group was 0.485. Heterozygosity, polymorphism information content, and the power of discrimination were 0.477, 0.362, and 0.612, respectively. The combined power of discrimination and the combined power of exclusion reached to 0.99999999999999999669 and 0.999962965, respectively. The results showed that 57 InDels polymorphisms have high genetic polymorphisms in the Yi ethnic group. The 57 InDels could be used for forensic individual identification, paternity testing, and intercontinental population discrimination, with the potential for use in biogeographic ancestry inference.
DOI: 10.6084/m9.figshare.25869514
title: Unravelling the complex interplay of cuproptosis, lncRNAs, and immune infiltration in Alzheimer’s disease: a step towards novel therapeutic targets

description: Cuproptosis, a type of cell death involving copper ion accumulation and oxidative stress, has been implicated in the development of Alzheimer’s disease (AD). This study aimed to explore the potential mechanisms and roles of cuproptosis-related genes (CRGs), long non-coding RNAs (lncRNAs), and immune cells in the development of cuproptosis in AD. Gene expression profiles of AD were acquired from the Gene Expression Omnibus (GEO) database, and differential analysis was conducted to identify CRGs. Random Forest (RF) modelling was employed to select the most crucial CRGs, which were subsequently validated in the test set. A nomogram model was created to predict AD risk and categorise AD subtypes based on the identified CRGs. A lncRNA-related ceRNA network was built, and immune cell infiltration analysis was conducted. Twelve differentially expressed CRGs were identified in the AD dataset. The RF model pinpointed the five most critical CRGs, which were validated in the test set with an AUC of 0.90. A lncRNA-related ceRNA network was developed, and immune cell infiltration analysis revealed high levels of M1 macrophages and mast cells, along with low levels of memory B cells in AD samples. Correlation analysis unveiled associations between CRGs, lncRNAs, and differentially infiltrating immune cells. This research offers insights into the potential mechanisms and roles of CRGs, lncRNAs, and immune cells in the development of cuproptosis in AD. The identified CRGs and lncRNAs may serve as potential therapeutic targets for AD, and the nomogram model may assist in early AD diagnosis and subtyping.
DOI: 10.6084/m9.figshare.25869514.v1
title: Unravelling the complex interplay of cuproptosis, lncRNAs, and immune infiltration in Alzheimer’s disease: a step towards novel therapeutic targets

description: Cuproptosis, a type of cell death involving copper ion accumulation and oxidative stress, has been implicated in the development of Alzheimer’s disease (AD). This study aimed to explore the potential mechanisms and roles of cuproptosis-related genes (CRGs), long non-coding RNAs (lncRNAs), and immune cells in the development of cuproptosis in AD. Gene expression profiles of AD were acquired from the Gene Expression Omnibus (GEO) database, and differential analysis was conducted to identify CRGs. Random Forest (RF) modelling was employed to select the most crucial CRGs, which were subsequently validated in the test set. A nomogram model was created to predict AD risk and categorise AD subtypes based on the identified CRGs. A lncRNA-related ceRNA network was built, and immune cell infiltration analysis was conducted. Twelve differentially expressed CRGs were identified in the AD dataset. The RF model pinpointed the five most critical CRGs, which were validated in the test set with an AUC of 0.90. A lncRNA-related ceRNA network was developed, and immune cell infiltration analysis revealed high levels of M1 macrophages and mast cells, along with low levels of memory B cells in AD samples. Correlation analysis unveiled associations between CRGs, lncRNAs, and differentially infiltrating immune cells. This research offers insights into the potential mechanisms and roles of CRGs, lncRNAs, and immune cells in the development of cuproptosis in AD. The identified CRGs and lncRNAs may serve as potential therapeutic targets for AD, and the nomogram model may assist in early AD diagnosis and subtyping.
DOI: 10.6084/m9.figshare.26370571
title: Genetic polymorphism of 27 Y-STR loci in Kazakh populations from Central Kazakhstan

description: The haplotypes from Northern, Southern, Eastern, and Western Kazakhstan, analysed for 27 Y-STR loci, have been contributed to the Y-Chromosome STR Haplotype Reference Database, while the genetic profile of Central Kazakhstan remains inadequately explored. To investigate the genetic diversity of 27 Y-STR loci in the Kazakh populations from Central Kazakhstan. A total of 112 unrelated Central Kazakh males were genotyped <i>via</i> the Yfiler Plus kit. Data analysis yielded haplotype and allele frequencies, and forensic parameters. Genetic distances were graphically represented by a multidimensional scaling plot, with genetic linkages further elucidated through Nei’s distance dendrograms and Median-joining networks. A total of 102 haplotypes were detected, of which 96 were unique. The haplotype diversity and discrimination capacity were 0.997 and 0.91, respectively. Central Kazakhstan displays a unique cluster in analyses, underscoring its distinct Y-chromosome diversity compared to other Kazakh regions. The analysis of the Naiman tribe, predominantly residing in Central, Southern and Eastern Kazakhstan, revealed three genetic clusters of distinct haplogroups associated with their clans. The identified haplotypes will enhance the existing reference database for Y-chromosomal studies in Kazakhstan, offering a robust tool for future research in population genetics, forensic science and genetic genealogy.
DOI: 10.6084/m9.figshare.26370571.v1
title: Genetic polymorphism of 27 Y-STR loci in Kazakh populations from Central Kazakhstan

description: The haplotypes from Northern, Southern, Eastern, and Western Kazakhstan, analysed for 27 Y-STR loci, have been contributed to the Y-Chromosome STR Haplotype Reference Database, while the genetic profile of Central Kazakhstan remains inadequately explored. To investigate the genetic diversity of 27 Y-STR loci in the Kazakh populations from Central Kazakhstan. A total of 112 unrelated Central Kazakh males were genotyped <i>via</i> the Yfiler Plus kit. Data analysis yielded haplotype and allele frequencies, and forensic parameters. Genetic distances were graphically represented by a multidimensional scaling plot, with genetic linkages further elucidated through Nei’s distance dendrograms and Median-joining networks. A total of 102 haplotypes were detected, of which 96 were unique. The haplotype diversity and discrimination capacity were 0.997 and 0.91, respectively. Central Kazakhstan displays a unique cluster in analyses, underscoring its distinct Y-chromosome diversity compared to other Kazakh regions. The analysis of the Naiman tribe, predominantly residing in Central, Southern and Eastern Kazakhstan, revealed three genetic clusters of distinct haplogroups associated with their clans. The identified haplotypes will enhance the existing reference database for Y-chromosomal studies in Kazakhstan, offering a robust tool for future research in population genetics, forensic science and genetic genealogy.
DOI: 10.6084/m9.figshare.25040901
title: Paediatric dominant and non-dominant handgrip reference curves and the association with body composition

description: Lack of paediatric reference data limits the utility of handgrip strength as a measure of fitness and well-being. To develop paediatric handgrip reference curves and evaluate associations with body size and composition and race/ethnicity group. Handgrip, body size and composition data were obtained from National Health and Nutrition Examination Survey 2011–2014 participants aged 6–20 years. Densitometry-derived fat and appendicular lean soft tissue mass index Z-scores (FMIZ, ALSTMIZ) were generated in participants &gt;8 years. Dominant and non-dominant handgrip reference curves were created using the LMS method. Analyses included sample weights to produce nationally representative estimates. Differences in handgrip strength according to hand dominance increased with age. Handgrip strength was associated with height and arm length Z-scores (<i>R</i> = 0.42 to 0.47) and ALSTMIZ (<i>R</i> = 0.54). Handgrip strength was higher in the non-Hispanic Black group and lower in the Mexican American compared to non-Hispanic White group. Group differences were attenuated when adjusted for height, arm length or ALSTMIZ. Paediatric handgrip reference curves were generated from which individual Z-scores can be calculated separately for dominant versus non-dominant hand and adjusted for body size. Association with ALSTMIZ suggests handgrip Z-score may be used as a measure of functional body composition.
DOI: 10.6084/m9.figshare.25040901.v1
title: Paediatric dominant and non-dominant handgrip reference curves and the association with body composition

description: Lack of paediatric reference data limits the utility of handgrip strength as a measure of fitness and well-being. To develop paediatric handgrip reference curves and evaluate associations with body size and composition and race/ethnicity group. Handgrip, body size and composition data were obtained from National Health and Nutrition Examination Survey 2011–2014 participants aged 6–20 years. Densitometry-derived fat and appendicular lean soft tissue mass index Z-scores (FMIZ, ALSTMIZ) were generated in participants &gt;8 years. Dominant and non-dominant handgrip reference curves were created using the LMS method. Analyses included sample weights to produce nationally representative estimates. Differences in handgrip strength according to hand dominance increased with age. Handgrip strength was associated with height and arm length Z-scores (<i>R</i> = 0.42 to 0.47) and ALSTMIZ (<i>R</i> = 0.54). Handgrip strength was higher in the non-Hispanic Black group and lower in the Mexican American compared to non-Hispanic White group. Group differences were attenuated when adjusted for height, arm length or ALSTMIZ. Paediatric handgrip reference curves were generated from which individual Z-scores can be calculated separately for dominant versus non-dominant hand and adjusted for body size. Association with ALSTMIZ suggests handgrip Z-score may be used as a measure of functional body composition.
DOI: 10.6084/m9.figshare.25040898
title: Forensic parameters and population analysis of 21 autosomal STR loci in the Wuhu Han population from Anhui Province, East China

description: At present, there are no available genetic data on the AGCU EX22 Kit from the Wuhu Han population. This study investigates the applicability of the AGCU EX22 kit, designed for the Chinese population for forensic analysis and population genetics of the Wuhu Han population. Bloodstains from 1565 unrelated healthy individuals in Wuhu city, Anhui Province, were collected for analysis. The AGCU EX22 kit was used for amplification, and capillary electrophoresis was used to separate the amplification products. Allele frequencies and forensic parameters were determined. The Wuhu Han population was compared to 10 reference populations through genetic distance, a phylogenetic neighbor-joining tree and principal component analysis. In total, 281 alleles and 1187 genotypes were observed. No significant deviations from Hardy-Weinberg equilibrium at any locus were found after Bonferroni’s correction. The 21 autosomal short tandem repeat (STR) genetic markers exhibited high informativeness and polymorphism. The cumulative power of discrimination and power of exclusion were 0.999999999999999999999999913380 and 0.999999996752339, respectively. Population comparisons revealed a genetic affinity between Wuhu Han and southern Han populations, except for the Guangdong Han population, which aligned with the traditional geographical division in China. The AGCU EX22 Kit, containing 21 STR loci, is suitable for forensic application and population genetics studies in the Wuhu Han population.
DOI: 10.6084/m9.figshare.25040898.v1
title: Forensic parameters and population analysis of 21 autosomal STR loci in the Wuhu Han population from Anhui Province, East China

description: At present, there are no available genetic data on the AGCU EX22 Kit from the Wuhu Han population. This study investigates the applicability of the AGCU EX22 kit, designed for the Chinese population for forensic analysis and population genetics of the Wuhu Han population. Bloodstains from 1565 unrelated healthy individuals in Wuhu city, Anhui Province, were collected for analysis. The AGCU EX22 kit was used for amplification, and capillary electrophoresis was used to separate the amplification products. Allele frequencies and forensic parameters were determined. The Wuhu Han population was compared to 10 reference populations through genetic distance, a phylogenetic neighbor-joining tree and principal component analysis. In total, 281 alleles and 1187 genotypes were observed. No significant deviations from Hardy-Weinberg equilibrium at any locus were found after Bonferroni’s correction. The 21 autosomal short tandem repeat (STR) genetic markers exhibited high informativeness and polymorphism. The cumulative power of discrimination and power of exclusion were 0.999999999999999999999999913380 and 0.999999996752339, respectively. Population comparisons revealed a genetic affinity between Wuhu Han and southern Han populations, except for the Guangdong Han population, which aligned with the traditional geographical division in China. The AGCU EX22 Kit, containing 21 STR loci, is suitable for forensic application and population genetics studies in the Wuhu Han population.