DOI: 10.5061/dryad.gf1vhhmxj
title: Data from: Spatiotemporal changes in Netrin/DSCAM1 signaling dictate axonal projection direction in Drosophila small ventral lateral clock neurons

description: Axon projection is a spatial and temporal-specific process in which the
 growth cone receives environmental signals guiding axons to their final
 destination. However, the mechanisms underlying changes in axonal
 projection direction without well-defined landmarks remain elusive. Here,
 we present evidence showcasing the dynamic nature of axonal projections in
 Drosophila's small ventral lateral clock neurons (s-LNvs). Our
 findings reveal that these axons undergo an initial vertical projection in
 the early larval stage, followed by a subsequent transition to a
 horizontal projection in the early-to-mid third instar larvae. The
 vertical projection of s-LNv axons correlates with mushroom body calyx
 expansion, while the s-LNv-expressed Down syndrome cell adhesion molecule
 (Dscam1) interacts with Netrins to regulate the horizontal projection.
 During a specific temporal window, locally newborn dorsal clock neurons
 (DNs) secrete Netrins, facilitating the transition of axonal projection
 direction in s-LNvs. Our study establishes a compelling in vivo model to
 probe the mechanisms of axonal projection direction switching in the
 absence of clear landmarks. These findings underscore the significance of
 dynamic local microenvironments in the complementary regulation of axonal
 projection direction transitions.
description: # Data from: Spatiotemporal changes in Netrin/DSCAM1 signaling dictate
 axonal projection direction in Drosophila small ventral lateral clock
 neurons This dataset contains experimental data from a study aimed at
 investigating the development process of axon projection in s-LNvs. The
 data includes measurements of vertical axon projection distances and
 horizontal projection A.U. (arbitrary units) under different experimental
 conditions. The results of this study are discussed in detail in our
 relevant publication. #### **Description of the data and file structure**
 The xlsx data supports Figures 1, 2, 3, 4, 5, 6, Figure 2-Figure
 supplement 1, Figure 3-figure supplement 1, Figure 3-figure supplement 2,
 Figure 4-figure supplement 1 Figure 5-figure supplement 1, Figure 6-figure
 supplement 1. **Figure1-source data 1 Related to figure1B**:  data
 underlying in the main text. This file contains average vertical
 projection length at different developmental stages. Figure 1B: Average
 vertical projection length at different developmental stages * Column B: 8
 h - Column C: 24 h * Column D: 48 h - Column E: 72 h * Column F: 96 h *
 Column G: 120 h **Figure1-source data 2 Related to Figure 1C**:  data
 underlying in the main text. This file contains average horizontal
 projection (A.U.) at different developmental stages. Figure 1C: Average
 horizontal projection at different developmental stages * Column B: 8 h -
 Column C: 24 h * Column D: 48 h - Column E: 72 h * Column F: 96 h - Column
 G: 120 h **Figure2-source data 1 Related to figure2B**:  data underlying
 in the main text. This file contains three tabs. Figure 2B: Development of
 vertical projection process alongside Mushroom Body growth Total vertical
 projection distance * Column B: 24 h - Column C: 48 h * Column D: 72 h -
 Column E: 96 h * Column F: 120 h **OK107-GAL4 calyx area** * Column I: 24
 h - Column J: 48 h * Column K: 72 h - Column L: 96 h * Column M: 120 h
 **201Y-GAL4 calyx area** * Column O: 24 h - Column P: 48 h * Column Q: 72
 h - Column R: 96 h * Column S: 120 h **Figure2-source data 2 Related to
 figure2E**:  data underlying in the main text. This file includes data on
 the MB under both ablation and non-ablation treatments, with vertical
 projection distances * Column A: 24 h no ablation - Column B: 24 h
 ablation * Column C: 48 h no ablation - Column D: 48 h ablation * Column
 E: 72 h no ablation - Column F: 72 h ablation **Figure2-supplement figure1
 source data 1**: data underlying in the supplement figure. This file
 includes data on the MB calyx area under both ablation and non-ablation
 treatments * Column A: 24 h no ablation - Column B: 24 h ablation * Column
 C: 48 h no ablation - Column D: 48 h ablation * Column E: 72 h no ablation
 - Column F: 72 h ablation **Figure3-source data 1 Related to figure3B**: 
 data underlying in the main text. This file includes the vertical
 projection distances and related statistical methods for both
 non-knockdown and knockdown Dscam1 treatments.Figure 3B: Critical role of
 Dscam1 in horizontal projection * Column A: Pdf &gt;GFP - Column B: Pdf
 &gt;GFP, Dscam1RNAi **Figure3-source data 2 Related to figure3C**:  data
 underlying in the main text. This file includes the horizontal projection
 A.U. and related statistical methods for both non-knockdown and knockdown
 Dscam1 treatments.Figure 3C: Critical role of Dscam1 in horizontal
 projection * Column A: Pdf &gt;GFP - Column B: Pdf &gt;GFP, Dscam1RNAi
 **Figure3-source data 3 Related to figure3E**:  data underlying in the
 main text. This file includes the horizontal projection A.U. and related
 statistical methods for different Dscam1 mutant fly. * Column A: w1118 -
 Column B: Dscam1 21/+ * Column C: Dscam1 1/+ - Column D: Dscam1 05518/+ *
 Column E: Dscam1 21/05518* - Column F: Dscam1 21/1 * Column G: Dscam1
 05518  **Figure3-supplement figure1 source data 1**: data underlying in
 the supplement figure. This file includes the vertical projection
 distances and related statistical methods for both non-knockdown and
 knockdown Dscam1 treatments. * Column A: Clk856-Gal4 &gt;GFP - Column B:
 Clk856-Gal4 &gt;GFP, Dscam1RNAi **Figure3-supplement figure1 source data
 2**: data underlying in the supplement figure. This file includes the
 horizontal projection A.U. and related statistical methods for both
 non-knockdown and knockdown Dscam1 treatments. * Column A: Clk856-Gal4
 &gt;GFP - Column B: Clk856-Gal4 &gt;GFP, Dscam1RNAi  **Figure3-supplement
 figure2 source data 1**: data underlying in the supplement figure. This
 file includes the horizontal projection A.U.and related statistical
 methods for Pdf-GAL4 knockdown Dscam1 interaction molecule and
 cytoskeleton-associated regulatory proteins * Column A: pdf-GAL4/+ -
 Column B: pdf-GAL4 &gt;tsrRNAi * Column C: pdf-GAL4 &gt;chicRNAi - Column
 D: pdf-GAL4 &gt;SH3PX1RNAi * Column E: pdf-GAL4 &gt;DockRNAi - Column F:
 pdf-GAL4 &gt; PakRNAi **Figure4-source data 1 Related to figure4B**:  data
 underlying in the main text. This file includes the horizontal projection
 A.U. and related statistical methods for netrins mutant * Column A: w1118
 - Column B: NetAΔ * Column C: NetBΔ - Column D: NetABΔi **Figure4-source
 data 2 Related to figure4D**:  data underlying in the main text. This file
 includes the horizontal projection A.U. and related statistical methods
 for nSyb-GAL4 and repo-GAL4 knockdown Netrins * Column A: UAS-dicer;
 nSyb-GAL4/w1118 - Column B: UAS-dicer; nSyb-GAL4/Netrins RNAi * Column C:
 UAS-dicer; repo-GAL4/w1118 - Column D: UAS-dicer; repo- GAL4/Netrins RNAi 
 **Figure4-supplement figure1 source data 1**: data underlying in the
 supplement figure. This file includes the horizontal projection A.U. and
 related statistical methods for tubulin-GAL4 knockdown Netrins or slit *
 Column A: tubulin-GAL4/W1118 - Column B: tubulin-GAL4/Netrins RNAi *
 Column C: tubulin-GAL4/slit RNAi **Figure5-source data 1 Related to
 figure5B**:  data underlying in the main text. This file includes the
 horizontal projection A.U. and related statistical methods for OK107-GAL4
 and per-GAL4,pdf-GAL80;UAS-GFP* knockdown Netrins * Column A:
 OK107-GAL4/w1118 - Column B: OK107-GAL4 /Netrins RNAi * Column C:
 per-GAL4,pdf-GAL80;UAS-GFP/w1118 - Column D:
 per-GAL4,pdf-GAL80;UAS-GFP/Netrins RNAi **Figure5-source data 2 Related to
 figure5D**:  data underlying in the main text. This file includes the
 horizontal projection A.U. and related statistical methods for DN ablation
 at different developmental stage. * Column A: 72 h no ablation - Column B:
 72 h ablation * Column C: 96 h no ablation - Column D: 96 h ablation
 **Figure5-source data 3 Related to figure5F**:  data underlying in the
 main text. This file includes the horizontal projection A.U. and related
 statistical methods for per-GAL4, Pdf-GAL80 overexpress NetB in NetABΔ. *
 Column A: NetABΔ - Column B: NetABΔ;per-GAL4 * Column C: NetABΔ;OE-NetB
 **Figure5-supplement figure1 source data 1**: data underlying in the
 supplement figure. This file includes the horizontal projection A.U. and
 related statistical methods for both ablated DN or crz+ neuron * Column A:
 per-GAL4,Pdf-GAL80 No ablation - Column B: per-GAL4,Pdf-GAL80 Ablation *
 Column C: crz-GAL4 No ablation - Column D: crz-GAL4 Ablation
 **Figure6-source data 1 Related to figure6B**:  data underlying in the
 main text. This file includes the cell numbers at different developmental
 stage. * Column A: 24 h - Column B: 48 h * Column C: 72 h - Column D: 96 h
 **Figure6-supplement figure1 source data 1**: data underlying in the
 supplement figure. This file includes the horizontal projection A.U. and
 related statistical methods for both R78G02-GAL4 overexpressed netrin. *
 Column A: R78G02/+ - Column B: R78G02 &gt;UAS-NetA * Column C: R78G02
 &gt;UAS-NetB