cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11390469
title: Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model

description: Abstract The poly (adenosine diphosphate (ADP)-ribosyl) polymerase inhibitors (PARPi) selectively kill cancer cells with BRCA1 or BRCA2 (BRCA)-mutations. It has been proposed that cell death induction after PARPi depends on unrepaired double strand breaks (DSBs) that accumulate due to the homologous recombination deficiency of BRCA-mutated cells. Such accumulation of DSBs is inferred mainly from the high levels of DNA damage markers like phosphorylated histone H2AX. Herein, we developed a model of isogenic cell lines to show that depletion of BRCA causes PARPi-triggered cell death, replication stress (phosphorylated-H2AX and 53BP1 foci), and genomic instability. However, persistent DSBs accumulation was not detected under the same experimental conditions. Hence, at least in this cellular model, the trigger for cell death in PARPi-treated BRCA-depleted samples is not the accumulation of unrepaired DSBs. Instead, cell death better correlates with a rapid and aberrant resolution of DSBs by error-prone pathways that leads to severe chromosomic aberrations. Therefore, our results suggest that in PARPi-treated BRCA-deficient cells, chromosome aberrations may dually trigger both genomic instability and cell death.
DOI: 10.6084/m9.figshare.11390478
title: Identification of populations and hybrid combinations of maize for in natura consumption

description: ABSTRACT Brazil is one of the leading countries in the production of maize (Zea mays), with great potential for growing green maize, which has a superior commercial value in relation to maize marketed in the form of grains. Although important, the availability of cultivars recommended for the production of green maizeis still very scarce. The objectives of the present study were to estimate genetic parameters and to identify promising hybrid combinations for the development of new green maize cultivars to farmers. In the summer crop of 2016/17, ten hybrid combinations obtained through a complete diallel of five maize populations, with attributes for in natura consumption, were evaluated in two sites of the state of São Paulo, Brazil: the Instituto Agronômico(IAC) in Campinas and Tatuí. A randomized block design was used with two additional checks, with three replications, in plots with four five-meter rows spaced by 0.9 m in Campinas and 0.8 m in Tatuí, with 5 plants per meter. The following agronomic traits were assessed: grain yield, ear yield with straw and ear yield without straw, using Griffing’s method 4. Significant effects of genotypes, environments and interaction genotypes × environments were detected for all traits. Estimates of the general combining ability led to the selection of populations P2, P4, and P5 as the ones with a higher concentration of favorable alleles for the characters evaluated. Estimates of specific combining ability and improved grain yield performance allowed P2xP3 to be selected as the most promising for production of green maize.
DOI: 10.6084/m9.figshare.11390517.v1
title: The gravitational deflection of light: From Newton to Einstein

description: Abstract Evidence of light bending due to gravitational effects is an important milestone in the history of science because measurements of light deflection in astronomical observations during the 1919 eclipse confirmed the value predicted by Einstein's theory of general relativity in 1915. This had an extraordinary repercussion in the press, which led Einstein to worldwide recognition and to becoming the most famous scientist in the non-scientific media of the twentieth century. However, light deflection had been a matter of attention since Newton. This paper discusses the advances and calculations performed by Einstein's predecessors and details his trajectory in the development of relativity theory until the exact determination of the gravitational deflection of light.
DOI: 10.6084/m9.figshare.11390436
title: ADP-ribosylation: from molecular mechanisms to human disease

description: Abstract Post-translational modification of proteins by ADP-ribosylation, catalysed by poly (ADP-ribose) polymerases (PARPs) using NAD+ as a substrate, plays central roles in DNA damage signalling and repair, modulates a range of cellular signalling cascades and initiates programmed cell death by parthanatos. Here, we present mechanistic aspects of ADP-ribose modification, PARP activation and the cellular functions of ADP-ribose signalling, and discuss how this knowledge is uncovering therapeutic avenues for the treatment of increasingly prevalent human diseases such as cancer, ischaemic damage and neurodegeneration.
DOI: 10.6084/m9.figshare.11390454
title: Religious/spiritual coping and level of hope in patients with cancer in chemotherapy

description: ABSTRACT Aim: To demonstrate the relationship between religious/spiritual coping and hope in cancer patients undergoing chemotherapy. Method: This is a cross-sectional, descriptive study with a quantitative approach performed in a reference outpatient clinic in Caruaru, PE, between August and October 2017. A total of 82 cancer patients undergoing chemotherapy were included in the study, using the brief religious/spiritual coping scale (RCOPE-Brief) and the Herth Hope Scale (HHS). Results: The sample presented mean positive RCOPE scores (3.03 ± 0.41) and the level of hope was considered high (42.7 points ± 3.67). Patients who had a high RCOPE score were found to have a higher mean of Herth’s level of hope (44.12 points). Conclusion: This study becomes relevant to nursing professionals by encouraging care that takes into account the patient’s spiritual dimension in order to stimulate positive mechanisms of religious coping and, consequently, raise the levels of hope.
DOI: 10.6084/m9.figshare.11390469.v1
title: Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model

description: Abstract The poly (adenosine diphosphate (ADP)-ribosyl) polymerase inhibitors (PARPi) selectively kill cancer cells with BRCA1 or BRCA2 (BRCA)-mutations. It has been proposed that cell death induction after PARPi depends on unrepaired double strand breaks (DSBs) that accumulate due to the homologous recombination deficiency of BRCA-mutated cells. Such accumulation of DSBs is inferred mainly from the high levels of DNA damage markers like phosphorylated histone H2AX. Herein, we developed a model of isogenic cell lines to show that depletion of BRCA causes PARPi-triggered cell death, replication stress (phosphorylated-H2AX and 53BP1 foci), and genomic instability. However, persistent DSBs accumulation was not detected under the same experimental conditions. Hence, at least in this cellular model, the trigger for cell death in PARPi-treated BRCA-depleted samples is not the accumulation of unrepaired DSBs. Instead, cell death better correlates with a rapid and aberrant resolution of DSBs by error-prone pathways that leads to severe chromosomic aberrations. Therefore, our results suggest that in PARPi-treated BRCA-deficient cells, chromosome aberrations may dually trigger both genomic instability and cell death.
DOI: 10.6084/m9.figshare.11390478.v1
title: Identification of populations and hybrid combinations of maize for in natura consumption

description: ABSTRACT Brazil is one of the leading countries in the production of maize (Zea mays), with great potential for growing green maize, which has a superior commercial value in relation to maize marketed in the form of grains. Although important, the availability of cultivars recommended for the production of green maizeis still very scarce. The objectives of the present study were to estimate genetic parameters and to identify promising hybrid combinations for the development of new green maize cultivars to farmers. In the summer crop of 2016/17, ten hybrid combinations obtained through a complete diallel of five maize populations, with attributes for in natura consumption, were evaluated in two sites of the state of São Paulo, Brazil: the Instituto Agronômico(IAC) in Campinas and Tatuí. A randomized block design was used with two additional checks, with three replications, in plots with four five-meter rows spaced by 0.9 m in Campinas and 0.8 m in Tatuí, with 5 plants per meter. The following agronomic traits were assessed: grain yield, ear yield with straw and ear yield without straw, using Griffing’s method 4. Significant effects of genotypes, environments and interaction genotypes × environments were detected for all traits. Estimates of the general combining ability led to the selection of populations P2, P4, and P5 as the ones with a higher concentration of favorable alleles for the characters evaluated. Estimates of specific combining ability and improved grain yield performance allowed P2xP3 to be selected as the most promising for production of green maize.
DOI: 10.6084/m9.figshare.11390430
title: Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition

description: ABSTRACT: Extraction conditions are an important factor in the process of obtaining bioactive compounds from plant matrix. These compounds differ structurally. Structures of phyto-compounds and their interactions with other food ingredient are not fully known, while these two aspects should play a significant role in extrahents choice and determination of extraction process conditions. Mulberry (Morus alba) is a plant growing in Asia, which fruits are rich in bioactive ingredients and high anti-oxidative potential. In our study we analyzed mulberry fruits extracts differing in the extra hent applied: acetone, methanol, ethanol and water. All tested extracts possessed rich polyphenolic composition and radical scavenging ability. The significant differences among the extracts in phenolic acids and flavonoids compositions were noticed, where the highest values were observed for acetone extract. The extrahent applied affects the antioxidative profile of tested samples, as well. The highest scavenging activity against ABTS was observed for acetone and ethanol extracts, while the poorest activity had water extract. Similar results were provided for ferrous ion reducing test and Fe chlating activity (acetone&gt;ethanol&gt;methanol&gt;water). These results are helpful when selecting solvents with appropriate bioactive compounds compositions and high phytochemical profiles to be used as ingredients in supplements, as well as in functional foods.
DOI: 10.6084/m9.figshare.11390436.v1
title: ADP-ribosylation: from molecular mechanisms to human disease

description: Abstract Post-translational modification of proteins by ADP-ribosylation, catalysed by poly (ADP-ribose) polymerases (PARPs) using NAD+ as a substrate, plays central roles in DNA damage signalling and repair, modulates a range of cellular signalling cascades and initiates programmed cell death by parthanatos. Here, we present mechanistic aspects of ADP-ribose modification, PARP activation and the cellular functions of ADP-ribose signalling, and discuss how this knowledge is uncovering therapeutic avenues for the treatment of increasingly prevalent human diseases such as cancer, ischaemic damage and neurodegeneration.
DOI: 10.6084/m9.figshare.11390454.v1
title: Religious/spiritual coping and level of hope in patients with cancer in chemotherapy

description: ABSTRACT Aim: To demonstrate the relationship between religious/spiritual coping and hope in cancer patients undergoing chemotherapy. Method: This is a cross-sectional, descriptive study with a quantitative approach performed in a reference outpatient clinic in Caruaru, PE, between August and October 2017. A total of 82 cancer patients undergoing chemotherapy were included in the study, using the brief religious/spiritual coping scale (RCOPE-Brief) and the Herth Hope Scale (HHS). Results: The sample presented mean positive RCOPE scores (3.03 ± 0.41) and the level of hope was considered high (42.7 points ± 3.67). Patients who had a high RCOPE score were found to have a higher mean of Herth’s level of hope (44.12 points). Conclusion: This study becomes relevant to nursing professionals by encouraging care that takes into account the patient’s spiritual dimension in order to stimulate positive mechanisms of religious coping and, consequently, raise the levels of hope.
DOI: 10.6084/m9.figshare.11390418
title: Simpósio Nacional de Diagnóstico de Enfermagem: building a knowledge field for Nursing

description: ABSTRACT Objective: to recount the story of the Simpósio Nacional de Diagnóstico de Enfermagem (National Nursing Diagnosis Symposium), from 1991 to 2018, describing the official themes and main recommendations; and reflect on the construction, in this process, of a specific field of knowledge for Nursing. Results: the manuscript became a historical study, using official documents resulting from the thirteen Symposiums conducted in the period from 1991 to 2018 as the primary source of empirical data. The outcomes were divided into two stages, from 1991 to 1992, when the event was linked to Interest Groups in Nursing Diagnosis; and from 1996 to 2018, when ABEn Nacional took over the organization and execution of the event. Final considerations: the socialization and exchange of knowledge about systematization of care, Nursing Process and nursing terminologies, themes focused on SINADEn, contributed decisively to the construction of a fruitful field of knowledge for Nursing.
DOI: 10.6084/m9.figshare.11390424
title: Lawsuits in health: an integrative review

description: ABSTRACT Objective: To analyze the national and international scientific evidence available in the literature on types of judicialization of health lawsuits. Method: Integrative review, which selected primary studies in the PubMed, LILACS, Web of Science and Scopus databases, with the Descriptors: judicial decisions, health’s judicialization, Saúde (in Portuguese), Health, and the keyword: Judicial Action. Results: 30 studies were selected. In Brazil, the majority were legal claims for medicines and the other for medical errors, requests for vaccines, supplies for diabetics, food compounds, surgical procedure, examinations, among others. In international studies, lawsuits were found for medication, benefit coverage and hospitalization for psychiatric treatment. Conclusion: It is evident that the most demanded type of lawsuit was access to the medication at an international level. It is still more noticeable the little discussion on this subject, demonstrating that judicialization of medicines can indicate a reality of Brazil.
DOI: 10.6084/m9.figshare.11390430.v1
title: Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition

description: ABSTRACT: Extraction conditions are an important factor in the process of obtaining bioactive compounds from plant matrix. These compounds differ structurally. Structures of phyto-compounds and their interactions with other food ingredient are not fully known, while these two aspects should play a significant role in extrahents choice and determination of extraction process conditions. Mulberry (Morus alba) is a plant growing in Asia, which fruits are rich in bioactive ingredients and high anti-oxidative potential. In our study we analyzed mulberry fruits extracts differing in the extra hent applied: acetone, methanol, ethanol and water. All tested extracts possessed rich polyphenolic composition and radical scavenging ability. The significant differences among the extracts in phenolic acids and flavonoids compositions were noticed, where the highest values were observed for acetone extract. The extrahent applied affects the antioxidative profile of tested samples, as well. The highest scavenging activity against ABTS was observed for acetone and ethanol extracts, while the poorest activity had water extract. Similar results were provided for ferrous ion reducing test and Fe chlating activity (acetone&gt;ethanol&gt;methanol&gt;water). These results are helpful when selecting solvents with appropriate bioactive compounds compositions and high phytochemical profiles to be used as ingredients in supplements, as well as in functional foods.
DOI: 10.6084/m9.figshare.11390415
title: Phytodesalination of a moderately saline soil combined with two inorganic amendments

description: ABSTRACT The shortage of water and the increasing salinity are the main limiting environmental factors that directly affect the establishment and the development of crops. In this research, phytodesalination capacity of Sesuvium verrucosum was evaluated alone and in combination with agricultural gypsum (CaSO4·2H2O) and Polisul-C, in order to remedy a moderately saline soil at greenhouse level and under nonleaching conditions. The treatments studied were the following: T1 (soil), T2 (soil + S. verrucosum), T3 (soil +S. verrucosum + Polisul-C), T4 (soil + S. verrucosum + CaSO4·2H2O). Polyvinyl chloride (PVC) tubes filled with 8 kg of clay soil with an electrical conductivity of the saturation paste extract (ECe) of6.21 dS.m-1 were used. Soil samples were analyzed to determine the ECe, and the soluble and interchangeable cations (Na+, K+, Ca2+ and Mg2+) content. Then, the halophytic plants were divided into root and aerial parts and the content of Na+, K+, Ca2+ and Mg2+ was determined. In summary, S. verrucosum showed potential to desalinate its rhizosphere. Moreover, S. verrucosum desalination capacity significantly increases when used in combination with either of the tested amendments. This increase occurred mainly in the upper layers of the clay soil (0-30 cm). CaSO4·2H2O was shown to be the most effective amendment, since a greater gain in biomass and a large accumulation of sodium (Na+) in the aerial part of S. verrucosum was observed as a consequence of the soil improved physico-chemical properties caused by this chemical.
DOI: 10.6084/m9.figshare.11390415.v1
title: Phytodesalination of a moderately saline soil combined with two inorganic amendments

description: ABSTRACT The shortage of water and the increasing salinity are the main limiting environmental factors that directly affect the establishment and the development of crops. In this research, phytodesalination capacity of Sesuvium verrucosum was evaluated alone and in combination with agricultural gypsum (CaSO4·2H2O) and Polisul-C, in order to remedy a moderately saline soil at greenhouse level and under nonleaching conditions. The treatments studied were the following: T1 (soil), T2 (soil + S. verrucosum), T3 (soil +S. verrucosum + Polisul-C), T4 (soil + S. verrucosum + CaSO4·2H2O). Polyvinyl chloride (PVC) tubes filled with 8 kg of clay soil with an electrical conductivity of the saturation paste extract (ECe) of6.21 dS.m-1 were used. Soil samples were analyzed to determine the ECe, and the soluble and interchangeable cations (Na+, K+, Ca2+ and Mg2+) content. Then, the halophytic plants were divided into root and aerial parts and the content of Na+, K+, Ca2+ and Mg2+ was determined. In summary, S. verrucosum showed potential to desalinate its rhizosphere. Moreover, S. verrucosum desalination capacity significantly increases when used in combination with either of the tested amendments. This increase occurred mainly in the upper layers of the clay soil (0-30 cm). CaSO4·2H2O was shown to be the most effective amendment, since a greater gain in biomass and a large accumulation of sodium (Na+) in the aerial part of S. verrucosum was observed as a consequence of the soil improved physico-chemical properties caused by this chemical.
DOI: 10.6084/m9.figshare.11390418.v1
title: Simpósio Nacional de Diagnóstico de Enfermagem: building a knowledge field for Nursing

description: ABSTRACT Objective: to recount the story of the Simpósio Nacional de Diagnóstico de Enfermagem (National Nursing Diagnosis Symposium), from 1991 to 2018, describing the official themes and main recommendations; and reflect on the construction, in this process, of a specific field of knowledge for Nursing. Results: the manuscript became a historical study, using official documents resulting from the thirteen Symposiums conducted in the period from 1991 to 2018 as the primary source of empirical data. The outcomes were divided into two stages, from 1991 to 1992, when the event was linked to Interest Groups in Nursing Diagnosis; and from 1996 to 2018, when ABEn Nacional took over the organization and execution of the event. Final considerations: the socialization and exchange of knowledge about systematization of care, Nursing Process and nursing terminologies, themes focused on SINADEn, contributed decisively to the construction of a fruitful field of knowledge for Nursing.
DOI: 10.6084/m9.figshare.11390424.v1
title: Lawsuits in health: an integrative review

description: ABSTRACT Objective: To analyze the national and international scientific evidence available in the literature on types of judicialization of health lawsuits. Method: Integrative review, which selected primary studies in the PubMed, LILACS, Web of Science and Scopus databases, with the Descriptors: judicial decisions, health’s judicialization, Saúde (in Portuguese), Health, and the keyword: Judicial Action. Results: 30 studies were selected. In Brazil, the majority were legal claims for medicines and the other for medical errors, requests for vaccines, supplies for diabetics, food compounds, surgical procedure, examinations, among others. In international studies, lawsuits were found for medication, benefit coverage and hospitalization for psychiatric treatment. Conclusion: It is evident that the most demanded type of lawsuit was access to the medication at an international level. It is still more noticeable the little discussion on this subject, demonstrating that judicialization of medicines can indicate a reality of Brazil.
DOI: 10.6084/m9.figshare.11390277
title: SurgeryAnalysisPublic.sav

description: Dataset for: No evidence that that childhood genital surgery influences gender congruence in adults with atypical sex characteristics: Results of a combined quantitative analysis of 282 cases in 58 academic articles
DOI: 10.6084/m9.figshare.11390022
title: Sutcliffe et al Scientific Reports 2018 images

description: Images used for Sarcomere Organization Texture Analysis in the manuscript:<br>Matthew D. Sutcliffe, Philip M. Tan, Antonio Fernandez-Perez, Young-Jae Nam, Nikhil V. Munshi and Jeffrey J. Saucerman. (2018). High content analysis identifies unique morphological features of reprogrammed cardiomyocytes. Scientific Reports 8, 1258.<br>These images were acquired by Antonio Fernandez-Perez, Young-Jae Nam, and Nikhil V. Munshi.<br>SOTA code is at the GitHub repository:https://github.com/saucermanlab/SarcOrgTextureAnalysis<br><br>To reproduce analyses from the manuscript, place the Image folder within the directory for SOTA and run master.m.
DOI: 10.6084/m9.figshare.11389989
title: Supplementary Table 1.docx

description: <b>Supplementary Table 1.</b> 7 putative sites were predicted with these settings (80%) in sequence named<b> TSPAN8</b>
DOI: 10.6084/m9.figshare.11389989.v2
title: Supplementary Table 1.docx

description: <b>Supplementary Table 1.</b> 7 putative sites were predicted with these settings (80%) in sequence named<b> TSPAN8</b>
DOI: 10.6084/m9.figshare.11389989.v1
title: Supplementary Table 1.docx

description: <b>Supplementary Table 1.</b> 7 putative sites were predicted with these settings (80%) in sequence named<b> TSPAN8</b>
DOI: 10.6084/m9.figshare.11389824
title: Wallace_etal_Pcubensis.xlsx

description: Genotyping microsatellite data for Pseudoperonospora cubensis isolates infecting commercial and non-commercial cucurbit hosts in North Carolina
DOI: 10.6084/m9.figshare.11389824.v1
title: Wallace_etal_Pcubensis.xlsx

description: Genotyping microsatellite data for Pseudoperonospora cubensis isolates infecting commercial and non-commercial cucurbit hosts in North Carolina