cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22121237
title: Influence of the ionic strength on the physicochemical properties of methotrexate-loaded chitosan polyelectrolyte complexes

description: Abstract Polyelectrolyte complexes (PECs) as drug delivery systems are widely explored since they are easily obtained by coacervation and biopolymers can be associated. However, particle distribution is a challenging critical parameter that has been infrequently focused. This work evaluated the effect of NaCl concentration on the physicochemical properties of PECs based on chitosan and hypromellose loaded with methotrexate. The particle size, zeta potential and polydispersity index (PdI) were determined by DLS, besides of drug entrapment efficiency (EE) and in vitro drug release profile determination. Particle size decreased while NaCl concentration rised, achieving a narrower size distribution of (345±79 nm) and PdI (0.285±0.067) with 200 mmol/L NaCl. The higher the NaCl concentration, the lower the zeta potential at acid pH. The EE was kept similar ((28.2±4.5) %) from 0 to 300 mmol/L NaCl, while 400 mmol/L NaCl impaired the drug entrapment. The addition of (200 and 300) mmol/L NaCl did not affect the drug release profile, but it was faster with (100 or 400) mmol/L. In conclusion, the addition of 200 mmol/L NaCl reduced particle size and PdI with no changes in the EE and drug release. Therefore, the ionic strength plays an important role on PECs development.
DOI: 10.6084/m9.figshare.22121237.v1
title: Influence of the ionic strength on the physicochemical properties of methotrexate-loaded chitosan polyelectrolyte complexes

description: Abstract Polyelectrolyte complexes (PECs) as drug delivery systems are widely explored since they are easily obtained by coacervation and biopolymers can be associated. However, particle distribution is a challenging critical parameter that has been infrequently focused. This work evaluated the effect of NaCl concentration on the physicochemical properties of PECs based on chitosan and hypromellose loaded with methotrexate. The particle size, zeta potential and polydispersity index (PdI) were determined by DLS, besides of drug entrapment efficiency (EE) and in vitro drug release profile determination. Particle size decreased while NaCl concentration rised, achieving a narrower size distribution of (345±79 nm) and PdI (0.285±0.067) with 200 mmol/L NaCl. The higher the NaCl concentration, the lower the zeta potential at acid pH. The EE was kept similar ((28.2±4.5) %) from 0 to 300 mmol/L NaCl, while 400 mmol/L NaCl impaired the drug entrapment. The addition of (200 and 300) mmol/L NaCl did not affect the drug release profile, but it was faster with (100 or 400) mmol/L. In conclusion, the addition of 200 mmol/L NaCl reduced particle size and PdI with no changes in the EE and drug release. Therefore, the ionic strength plays an important role on PECs development.
DOI: 10.6084/m9.figshare.22121241
title: Approach of utilizing Artemisia herbs to treat covid-19

description: Abstract Huge plethora of studies pointed out the importance of several Artemisia herb molecules as treatment for many diseases. Among these natural substances, some molecules are known to counteract fever, inflammation, blood clotting and oxidative stress. Furthermore, they are dotted with activities against different strains of viruses including the SARS-coronaviruses. For these beneficial properties, these medicinal herbs are deemed as a potential candidate for covid-19 pandemic.
DOI: 10.6084/m9.figshare.22121241.v1
title: Approach of utilizing Artemisia herbs to treat covid-19

description: Abstract Huge plethora of studies pointed out the importance of several Artemisia herb molecules as treatment for many diseases. Among these natural substances, some molecules are known to counteract fever, inflammation, blood clotting and oxidative stress. Furthermore, they are dotted with activities against different strains of viruses including the SARS-coronaviruses. For these beneficial properties, these medicinal herbs are deemed as a potential candidate for covid-19 pandemic.
DOI: 10.6084/m9.figshare.22092894
title: UFSMFW 2101 - very early black oat cultivar with high yield potential

description: Abstract The black oat cultivar UFSMFW 2101, recommended for Northwest of the state of Rio Grande do Sul, is very early and can produce high dry matter and grain yields. In rotation systems, an immediate advantage is the very early cycle, with the possibility of anticipated sowing of the subsequent crop.
DOI: 10.6084/m9.figshare.22092894.v1
title: UFSMFW 2101 - very early black oat cultivar with high yield potential

description: Abstract The black oat cultivar UFSMFW 2101, recommended for Northwest of the state of Rio Grande do Sul, is very early and can produce high dry matter and grain yields. In rotation systems, an immediate advantage is the very early cycle, with the possibility of anticipated sowing of the subsequent crop.
DOI: 10.6084/m9.figshare.22121243
title: Modified bilateral fasciaperichondrial flap for prominent ear correction

description: Abstract Objective: Various techniques have been described in the literature for prominent ear correction. These cartilage-preserving or cartilage-shaping techniques have their own advantages and disadvantages. We aim to achieve aesthetic and stable results with low complication rates using combinations of these methods. Herein, we present our results of prominent ear surgery with a modified bilateral fasciaperichondrial flap in combination with concha-mastoid and concha-scaphal sutures. Methods: Patients whose surgeries included a modified bilateral fasciaperichondrial flap for prominent ear deformities were included in the study. Patients’ demographic data, pre- and postoperative Concha-Mastoid Angle (CMA) and upper-middle Helix-Mastoid Distances (HMD), follow-up time, complications, secondary operations, and postoperative Visual Analogue Scale (VAS) results were evaluated. With a postauricular fish-mouth incision, the bilateral fasciaperichondrial flap was planned into two: proximal- and distal-based. They were then elevated from the cartilage subperichondrially on the proximal side and supraperichondrially on the distal side. Concha-scaphal sutures were used to form an antihelical rim along with concha-mastoid sutures to reduce the concha-mastoid angle. Conchal cartilage resection was done if needed. Then, the bilateral fasciaperichondrial flaps were sutured together to cover the concha-mastoid and concha-scaphal sutures. Results: Between May 2017 and May 2021, 32 ears of 17 patients were operated on dueto prominent ear deformity. No hematoma or infection was observed in any patient, and there were no instances of recurrence, suture exposure, hypertrophic scars, or keloids. The satisfaction level of all patients was 8.2 ± 0.9 points on average according to the VAS. In the anthropometric measurements, a statistically significant difference was found between preoperative and postoperative sixth month CMA and HMD values. Conclusion: A combination of suture techniques and a modified bilateral fasciaperichondrial flap may be used in prominent ear cases, with low recurrence rates and high patient satisfaction. Level of evidence: III.
DOI: 10.6084/m9.figshare.22121243.v1
title: Modified bilateral fasciaperichondrial flap for prominent ear correction

description: Abstract Objective: Various techniques have been described in the literature for prominent ear correction. These cartilage-preserving or cartilage-shaping techniques have their own advantages and disadvantages. We aim to achieve aesthetic and stable results with low complication rates using combinations of these methods. Herein, we present our results of prominent ear surgery with a modified bilateral fasciaperichondrial flap in combination with concha-mastoid and concha-scaphal sutures. Methods: Patients whose surgeries included a modified bilateral fasciaperichondrial flap for prominent ear deformities were included in the study. Patients’ demographic data, pre- and postoperative Concha-Mastoid Angle (CMA) and upper-middle Helix-Mastoid Distances (HMD), follow-up time, complications, secondary operations, and postoperative Visual Analogue Scale (VAS) results were evaluated. With a postauricular fish-mouth incision, the bilateral fasciaperichondrial flap was planned into two: proximal- and distal-based. They were then elevated from the cartilage subperichondrially on the proximal side and supraperichondrially on the distal side. Concha-scaphal sutures were used to form an antihelical rim along with concha-mastoid sutures to reduce the concha-mastoid angle. Conchal cartilage resection was done if needed. Then, the bilateral fasciaperichondrial flaps were sutured together to cover the concha-mastoid and concha-scaphal sutures. Results: Between May 2017 and May 2021, 32 ears of 17 patients were operated on dueto prominent ear deformity. No hematoma or infection was observed in any patient, and there were no instances of recurrence, suture exposure, hypertrophic scars, or keloids. The satisfaction level of all patients was 8.2 ± 0.9 points on average according to the VAS. In the anthropometric measurements, a statistically significant difference was found between preoperative and postoperative sixth month CMA and HMD values. Conclusion: A combination of suture techniques and a modified bilateral fasciaperichondrial flap may be used in prominent ear cases, with low recurrence rates and high patient satisfaction. Level of evidence: III.
DOI: 10.6084/m9.figshare.22121249
title: Smartphone-enabled otoscopy: method evaluation in clinical practice

description: Abstract Objective: To assess the diagnostic agreement between smartphone-enabled otoscopy and rigid otoendoscopy in tympanic membrane and middle ear diseases. Methods: A cross-sectional study was carried out to analyze otoscopies in patients seen at a general otorhinolaryngology (ORL) outpatient clinic, from June to December 2019. Eighty-three images of patients obtained from otoscopies performed through a smartphone device and a rigid endoscope were included, recorded, and stored for further analysis. The images were first analyzed by an experienced otologist, who assigned his diagnostic impression (defined as the gold standard) on each of the images. After this analysis, the images were displayed to a group of secondary raters (an experienced otorhinolaryngologist, a second-year resident in ORL, and a general practitioner). A questionnaire was applied related to each image. Results: There was high agreement between the smartphone device and the otoendoscopy images for all professionals, with a Kappa coefficient of 0.97 (p &lt; 0.001). The smartphone device showed a diagnostic sensitivity of 81.1% and a specificity of 71.1%. As for the otoendoscopy, it showed a sensitivity of 84.7% and a specificity of 72.4%. The image classification as “2 = Good” was the most frequent one, with 34.9% for otoendoscopy and 31.6% for the smartphone device. Conclusion: There was a high diagnostic agreement between smartphone device-guided otoscopy and the rigid otoendoscopy, demonstrating the feasibility of using this device in clinical practice. Level of Evidence: 3
DOI: 10.6084/m9.figshare.22121249.v1
title: Smartphone-enabled otoscopy: method evaluation in clinical practice

description: Abstract Objective: To assess the diagnostic agreement between smartphone-enabled otoscopy and rigid otoendoscopy in tympanic membrane and middle ear diseases. Methods: A cross-sectional study was carried out to analyze otoscopies in patients seen at a general otorhinolaryngology (ORL) outpatient clinic, from June to December 2019. Eighty-three images of patients obtained from otoscopies performed through a smartphone device and a rigid endoscope were included, recorded, and stored for further analysis. The images were first analyzed by an experienced otologist, who assigned his diagnostic impression (defined as the gold standard) on each of the images. After this analysis, the images were displayed to a group of secondary raters (an experienced otorhinolaryngologist, a second-year resident in ORL, and a general practitioner). A questionnaire was applied related to each image. Results: There was high agreement between the smartphone device and the otoendoscopy images for all professionals, with a Kappa coefficient of 0.97 (p &lt; 0.001). The smartphone device showed a diagnostic sensitivity of 81.1% and a specificity of 71.1%. As for the otoendoscopy, it showed a sensitivity of 84.7% and a specificity of 72.4%. The image classification as “2 = Good” was the most frequent one, with 34.9% for otoendoscopy and 31.6% for the smartphone device. Conclusion: There was a high diagnostic agreement between smartphone device-guided otoscopy and the rigid otoendoscopy, demonstrating the feasibility of using this device in clinical practice. Level of Evidence: 3
DOI: 10.6084/m9.figshare.22092769
title: ‘KU Gold’: a new yellow-fruit papaya cultivar in Thailand

description: Abstract KU Gold’ is a papaya cultivar with attractive yellow fruit and pale-yellow pulp during its immature stage. It can produce year-round sweet fruit containing a high amount of ascorbic acid during its ripe stage. It also has great potential as an ornamental plant due to its pale-yellow petioles and flowers.
DOI: 10.6084/m9.figshare.22092769.v1
title: ‘KU Gold’: a new yellow-fruit papaya cultivar in Thailand

description: Abstract KU Gold’ is a papaya cultivar with attractive yellow fruit and pale-yellow pulp during its immature stage. It can produce year-round sweet fruit containing a high amount of ascorbic acid during its ripe stage. It also has great potential as an ornamental plant due to its pale-yellow petioles and flowers.
DOI: 10.6084/m9.figshare.22092807
title: Genetic diversity of sweet corn inbred lines of public sectors in Thailand revealed by SSR markers

description: Abstract The Thailand governmental institutes play important roles in genetic improvement of sweet corn in Thailand. We assessed the genetic diversity of 268 sweet corn inbreds from three major institutes (NCSRC-KU, CNFCRC, and KKU) and three commercial hybrids using 20 SSR markers. The markers detected 224 alleles in total, with an average of 11.2 alleles per locus. Overall gene diversity was relatively high, being 0.7. Allelic richness, gene diversity, and heterozygosity in the inbreds among the three institutes were comparable. However, the inbreds from KKU possessed the greatest number of unique alleles and rare alleles, albeit they showed the highest percentage of genetic impurity. Neighbor-joining, principal coordinate, and STRUCTURE analyses showed that the inbreds were genetically different. Our findings provide insight into the breeding gene pool and population structures of the sweet corn germplasm of the public sector in Thailand, enhancing efficiency of sweet corn germplasm utilization for developing new varieties.
DOI: 10.6084/m9.figshare.22092807.v1
title: Genetic diversity of sweet corn inbred lines of public sectors in Thailand revealed by SSR markers

description: Abstract The Thailand governmental institutes play important roles in genetic improvement of sweet corn in Thailand. We assessed the genetic diversity of 268 sweet corn inbreds from three major institutes (NCSRC-KU, CNFCRC, and KKU) and three commercial hybrids using 20 SSR markers. The markers detected 224 alleles in total, with an average of 11.2 alleles per locus. Overall gene diversity was relatively high, being 0.7. Allelic richness, gene diversity, and heterozygosity in the inbreds among the three institutes were comparable. However, the inbreds from KKU possessed the greatest number of unique alleles and rare alleles, albeit they showed the highest percentage of genetic impurity. Neighbor-joining, principal coordinate, and STRUCTURE analyses showed that the inbreds were genetically different. Our findings provide insight into the breeding gene pool and population structures of the sweet corn germplasm of the public sector in Thailand, enhancing efficiency of sweet corn germplasm utilization for developing new varieties.
DOI: 10.6084/m9.figshare.22121255
title: Oil and Gas Companies - Are They Shifting to Renewables? A Study of Policy Mixes for Energy Transition in Brazil

description: ABSTRACT We argue that there is a need to advance further research that strengthens the analysis of policy mixes for the energy transition in major emerging economies. In this context, this article aims to answer the following question: How do Brazil’s policies favor or hinder an energy transition of oil and gas companies (O&G) to renewables? To achieve this purpose, we conducted literature and archival research and interviews with experts to analyze (a) Brazil’s energy policy mixes that address O&G and renewables issues; and (b) major O&G companies’ activities and perspectives that influence the energy transition. Results demonstrated that though some of the O&G companies have made significant renewables investments in the last years, they continue focusing on O&G activities. We discuss the main policy mix features that hinder the prioritization of renewables by these O&G companies and that can undermine a sustainable energy transition in Brazil.
DOI: 10.6084/m9.figshare.22121255.v1
title: Oil and Gas Companies - Are They Shifting to Renewables? A Study of Policy Mixes for Energy Transition in Brazil

description: ABSTRACT We argue that there is a need to advance further research that strengthens the analysis of policy mixes for the energy transition in major emerging economies. In this context, this article aims to answer the following question: How do Brazil’s policies favor or hinder an energy transition of oil and gas companies (O&G) to renewables? To achieve this purpose, we conducted literature and archival research and interviews with experts to analyze (a) Brazil’s energy policy mixes that address O&G and renewables issues; and (b) major O&G companies’ activities and perspectives that influence the energy transition. Results demonstrated that though some of the O&G companies have made significant renewables investments in the last years, they continue focusing on O&G activities. We discuss the main policy mix features that hinder the prioritization of renewables by these O&G companies and that can undermine a sustainable energy transition in Brazil.
DOI: 10.6084/m9.figshare.22121256
title: On-farm conservation of cassava in Cuiabá, Mato Grosso state, Brazil: ethnobotanical aspects and genetic diversity

description: ABSTRACT Family farmers are important in the on-farm conservation of cassava, since they maintain distinct ethnovarieties in their plantations. This research aimed to analyze ethnobotanical aspects and the genetic diversity of cassava ethnovarieties maintained by farmers in the Baixada Cuiabana, Mato Grosso state, Brazil, in the Rio dos Couros community. A semi-structured questionnaire was applied for the ethnobotanical characterization of the cassava ethnovarieties. Subsequently, young leaves of the cassava plants were collected for DNA extraction. The ethnobotanical survey identified 29 cassava ethnovarieties, with the most cultivated ones being Pão, Amarelona and Cacau. The farmers characterize the cassavas with the following characteristics: palatability, pulp color, origin and similarities with animals and vegetables. Genetic relationships were observed among the ethnovarieties and five groups were identified, with formation of subgroups. In these groupings, it is possible to highlight the isolation of the Matrinxã, Paraguainha and Broto Roxo ethnovarieties as the most genetically divergent ones.
DOI: 10.6084/m9.figshare.22121256.v1
title: On-farm conservation of cassava in Cuiabá, Mato Grosso state, Brazil: ethnobotanical aspects and genetic diversity

description: ABSTRACT Family farmers are important in the on-farm conservation of cassava, since they maintain distinct ethnovarieties in their plantations. This research aimed to analyze ethnobotanical aspects and the genetic diversity of cassava ethnovarieties maintained by farmers in the Baixada Cuiabana, Mato Grosso state, Brazil, in the Rio dos Couros community. A semi-structured questionnaire was applied for the ethnobotanical characterization of the cassava ethnovarieties. Subsequently, young leaves of the cassava plants were collected for DNA extraction. The ethnobotanical survey identified 29 cassava ethnovarieties, with the most cultivated ones being Pão, Amarelona and Cacau. The farmers characterize the cassavas with the following characteristics: palatability, pulp color, origin and similarities with animals and vegetables. Genetic relationships were observed among the ethnovarieties and five groups were identified, with formation of subgroups. In these groupings, it is possible to highlight the isolation of the Matrinxã, Paraguainha and Broto Roxo ethnovarieties as the most genetically divergent ones.
DOI: 10.6084/m9.figshare.22121261
title: Development and validation of a HILIC-HPLC-ELSD method for simultaneous determination of glucosamine hydrochloride and chondroitin sulfate in dietary supplements

description: Abstract The objective of the present study is to develop and validate a simple, selective and accurate hydrophilic interaction liquid chromatography - a high performance liquid chromatography incorporating an evaporative light scattering detector (HILIC-HPLC-ELSD) method for simultaneously determining glucosamine hydrochloride and chondroitin sulfate in dietary supplements. The chromatographic separation was carried out on a ZIC-HILIC column (150 mm x 4.6 mm x 5µm) in isocratic system mode with a mobile phase of acetonitrile, 30 mM ammonium formate and water (77:20:3, v/v/v) at pH 4.5, a column temperature of 35°C, a flow rate of 1 mL.min-1, and an injection volume of 5 µL. An evaporative light scattering (ELS) detector was used. Effective separation was achieved by means of analyte resolution of more than 1.5 with an analysis run time of approximately 20 minutes. The linearity of glucosamine hydrochloride and chondroitin sulfate ranged from 0.4 to 2.5 mg.mL-1. The limits of the detection and quantification of glucosamine hydrochloride were 20 and 80 mg.mL-1 respectively, while for chondroitin sulfate they were 80 and 400 mg.mL-1. All validation parameters satisfied the acceptance criteria in accordance with International Conference on Harmonisation (ICH) guidelines. The method was successfully applied to the assay of commercial dietary supplement samples.
DOI: 10.6084/m9.figshare.22121261.v1
title: Development and validation of a HILIC-HPLC-ELSD method for simultaneous determination of glucosamine hydrochloride and chondroitin sulfate in dietary supplements

description: Abstract The objective of the present study is to develop and validate a simple, selective and accurate hydrophilic interaction liquid chromatography - a high performance liquid chromatography incorporating an evaporative light scattering detector (HILIC-HPLC-ELSD) method for simultaneously determining glucosamine hydrochloride and chondroitin sulfate in dietary supplements. The chromatographic separation was carried out on a ZIC-HILIC column (150 mm x 4.6 mm x 5µm) in isocratic system mode with a mobile phase of acetonitrile, 30 mM ammonium formate and water (77:20:3, v/v/v) at pH 4.5, a column temperature of 35°C, a flow rate of 1 mL.min-1, and an injection volume of 5 µL. An evaporative light scattering (ELS) detector was used. Effective separation was achieved by means of analyte resolution of more than 1.5 with an analysis run time of approximately 20 minutes. The linearity of glucosamine hydrochloride and chondroitin sulfate ranged from 0.4 to 2.5 mg.mL-1. The limits of the detection and quantification of glucosamine hydrochloride were 20 and 80 mg.mL-1 respectively, while for chondroitin sulfate they were 80 and 400 mg.mL-1. All validation parameters satisfied the acceptance criteria in accordance with International Conference on Harmonisation (ICH) guidelines. The method was successfully applied to the assay of commercial dietary supplement samples.