DOI: 10.5061/dryad.f7m0cfz49
title: Data for: Surviving the serenade: how conflicting selection pressures shape the early stages of sexual signal diversification

description: Understanding how the early stages of sexual signal diversification
 proceed is critically important because these microevolutionary dynamics
 directly shape species trajectories and impact macroevolutionary patterns.
 Unfortunately, studying this is challenging because signals involve
 complex interactions between behavior, morphology, and physiology, much of
 which can only be measured in real time. In Hawaii, male Pacific field
 cricket song attracts both females and a deadly parasitoid fly. Over the
 past two decades, there has been a marked increase in signal variation in
 Hawaiian populations of these crickets, including novel male morphs with
 distinct mating songs. We capitalize on this rare opportunity to track
 changes in morph composition over time in a population with three novel
 morphs, investigating how mate and parasitoid attraction (components of
 sexual and natural selection) may shape signal evolution. We find dramatic
 fluctuation in morph proportions over the three years of the study,
 including the arrival and rapid increase of one novel morph. Natural and
 sexual selection pressures act differently among morphs, with some more
 attractive to mates and others more protected from parasitism.
 Collectively, our results suggest that differential protection from
 parasitism among morphs, rather than mate attraction, aligns with recent
 patterns of phenotypic change in the wild.
description: # README for data files accompanying Gallagher et al. (2024) “Surviving
 the serenade: how conflicting selection pressures shape the early stages
 of sexual signal diversification” ## Data File #1: song\_analysis.csv ---
 A data frame with calling song data from field-collected male Teleogryllus
 oceanicus crickets of different morphs. Data include unique male ID,
 morph, and two analyzed song characteristics. ### Description of the data
 and file structure --- Descriptions of column names: male_ID: unique male
 identifier. morph: discrete male morph. dominant_frequency: frequency (in
 Hz) with the greatest acoustic power. amplitude: amplitude ratio (relative
 to background noise). ## Data File #2: morph\_proportions\_over\_time.csv
 --- A data frame with male morph proportion data from field-sampled
 Teleogryllus oceanicus crickets from the first and last points of the
 study: 1 and 5. Compared using a Fisher's exact test. ### Description
 of the data and file structure --- Descriptions of column names:
 timepoint: the time point sampled (1 or 5). ancestral: the number of
 ancestral males sampled during that time point. rattling: the number of
 rattling males sampled during that time point. smallwing: the number of
 smallwing males sampled during that time point. curlywing: the number of
 curlywing males sampled during that time point. ## Data File #3:
 long\_distance\_mate\_attraction\_4way.csv --- A data frame with four-way
 long-distance calling song mate choice data from Teleogryllus oceanicus,
 used to measure the morph preferences of female crickets. These trials
 included "ancestral" song in four-way simultaneous trials. ###
 Description of the data and file structure --- Descriptions of column
 names: female_ID: unique female identifier. morph_choice: the morph that
 the female first contacted (chose). ## Data File #4:
 long\_distance\_mate\_attraction\_2way.csv --- A data frame with two-way
 long-distance calling song mate choice data from Teleogryllus oceanicus,
 used to measure the morph preferences of female crickets. ### Description
 of the data and file structure --- Descriptions of column names: femaleID:
 unique female identifier. song_a: the morph calling song played from
 speaker "a" during that trial. song_b: the morph calling song
 played from speaker "b" during that trial. morph_chosen_contact:
 the morph that the female first contacted (chose). If the female did not
 contact a speaker during the trial, this is left blank (cell is empty).
 morph_chosen_time: the morph that the female spent more time near. If the
 female did not spend any time near the speaker during the trial, this is
 left blank. trial_format: whether the trial consisted of two competing
 songs (competitive) or one song vs silence (solo). ## Data File #5:
 short\_distance\_mate\_attraction.csv --- A data frame with short-distance
 courtship mate choice data from Teleogryllus oceanicus, used to measure
 the morph preferences of female crickets. ### Description of the data and
 file structure --- Descriptions of column names: Male ID: unique male
 identifier. male_morph: the male's morph. femaleID: unique female
 identifier. mount.y.n: whether the female mounted the male.
 time_until_mount_after_stridulation: the time in seconds that it took for
 the female to mount the male after he began courting (stridulating). If
 the female did not mount the male during the trial, this is left blank
 (cell is empty). ## Data File #6:
 long\_distance\_fly\_attraction\_squares.csv --- A data frame with
 long-distance fly trapping data, collected using traps broadcasting
 various Teleogryllus oceanicus morph calling songs in the field, used to
 measure the morph preferences of the parasitoid fly, Ormia ochracea. Each
 row indicates an individual trap broadcasting a particular song loop. This
 experiment contained a "square" of traps playing ancestral,
 rattling, smallwing, and curlywing songs. ### Description of the data and
 file structure --- Descriptions of column names: morph: the calling song
 loop broadcast from that trap. flies_caught: the number of flies caught in
 that trap. ## Data File #7: long\_distance\_fly\_attraction\_triangles.csv
 --- A data frame with long-distance fly trapping data, collected using
 traps broadcasting various Teleogryllus oceanicus morph calling songs in
 the field, used to measure the morph preferences of the parasitoid fly,
 Ormia ochracea. Each row indicates an individual trap broadcasting a
 particular song loop. This experiment contained a "triangle" of
 traps playing rattling, smallwing, and curlywing songs. ### Description of
 the data and file structure --- Descriptions of column names: morph: the
 calling song loop broadcast from that trap. flies_caught: the number of
 flies caught in that trap. ## Data File #8:
 short\_distance\_fly\_attraction.csv --- A data frame with short-distance
 fly attraction data to various cricket (Teleogryllus oceanicus) morph
 songs, used to measure the morph preferences of the parasitoid fly, Ormia
 ochracea, at short-distances. ### Description of the data and file
 structure --- Descriptions of column names: numID: unique fly identifier.
 method: whether a purring song was included in the series of stimuli
 played for that individual fly (it didn't matter statistically so was
 excluded from the final model). song: the stimulus played during that
 trial. distance: the distance traveled (in cm) from the top to the bottom
 of the arena (max = 58). contact: whether the fly contacted the speaker
 during the trial. ## Data File #9: morph\_proportions\_lab\_vs\_field.csv
 --- A data frame with sampled male morph counts of Teleogryllus oceanicus,
 including time points 4 and 5 in the field, and time point 5 in the lab.
 Used to compare how morph composition changed between field and lab
 generations. ### Description of the data and file structure ---
 Descriptions of column names: timepoint_rearing: the time point (4 or 5)
 and rearing condition (field or lab). ancestral: the number of ancestral
 males sampled during that time point. rattling: the number of rattling
 males sampled during that time point. smallwing: the number of smallwing
 males sampled during that time point. curlywing: the number of curlywing
 males sampled during that time point. ## R code file:
 long\_distance\_cricket\_phonotaxis\_code.Rmd --- An R script for
 comparing female Teleogryllus oceanicus cricket attraction to the calling
 songs of various male morphs. Used to analyze the data in data file #4:
 long_distance_mate_attraction_2way.csv