cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.3381301.v1
title: Synthesis and cyclization of benzothiazole: review

description: Benzoheterocycles such as benzothiazoles, benzimid azoles and benzoxazoles can serve as unique and versatile scaffolds for experimental drug design. Among the all benzohaterocycles, benzothiazole has considerable place in research area especially in synthetic as well as inpharmaceutical chemistry because of its potent and significant pharmacological activities. Since, awide range of methods are available for synthesizing benzothiazole nucleus and its derivatives but areal need exists for new procedures that support many kinds of structural diversity and varioussubstitution. The present review deals with the common methods adopted and reported to focus thesynthesis as well as cyclisation of benzothiazole nucleus.<br>
DOI: 10.6084/m9.figshare.3381298
title: Method development and acid degradation study of doxofylline by rp-hplc and lc-ms/ms

description: A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and aciddegradation product. The method showed a linear response for concentrations in the range of 1-200 g/ml usingacetonitrile: formic Acid (90: 10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/minand retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness,robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. The acid degradation product as well as pathway was characterized by LC-MS/MS.RESULT AND DISCUSSION:A sensitive, selective, precise and accurate highperformance liquid chromatographic method of analysis ofdoxofylline in both as bulk drug and in formulation wasdeveloped and validated. The mobile phase consisted ofacetonitril: 0.05M formic Acid (90: 10v/v); pH-3.0. Thedetection wavelength was 274nm. This system was found to give the sharp peak for Doxofylline (RT-2.9). The methodwas validated as per ICH guideline (Table 1). Stabilityindicating assay method in which acid stress condition wasused for quantitative estimation of doxofylline in tabletformulation and identification of acid degradation productThe separation of drug from its degradation product wereoptimized by varying the ratio and/or nature of organicmodifier. Finally method was developed using same mobilephase composed of acetonitrile: formic Acid (90: 10); pH-3.0, in that both drug and degradation product showing good elution RT-2.9 (Doxofylline) and RT-4.8 (Aciddegradation product) and m/e-149. The acid degradationproduct and pure drug were identified by LC-MS/MS inorder to establish acid degradation pathway (fig 5 and 6).
DOI: 10.6084/m9.figshare.3381298.v1
title: Method development and acid degradation study of doxofylline by rp-hplc and lc-ms/ms

description: A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and aciddegradation product. The method showed a linear response for concentrations in the range of 1-200 g/ml usingacetonitrile: formic Acid (90: 10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/minand retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness,robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. The acid degradation product as well as pathway was characterized by LC-MS/MS.RESULT AND DISCUSSION:A sensitive, selective, precise and accurate highperformance liquid chromatographic method of analysis ofdoxofylline in both as bulk drug and in formulation wasdeveloped and validated. The mobile phase consisted ofacetonitril: 0.05M formic Acid (90: 10v/v); pH-3.0. Thedetection wavelength was 274nm. This system was found to give the sharp peak for Doxofylline (RT-2.9). The methodwas validated as per ICH guideline (Table 1). Stabilityindicating assay method in which acid stress condition wasused for quantitative estimation of doxofylline in tabletformulation and identification of acid degradation productThe separation of drug from its degradation product wereoptimized by varying the ratio and/or nature of organicmodifier. Finally method was developed using same mobilephase composed of acetonitrile: formic Acid (90: 10); pH-3.0, in that both drug and degradation product showing good elution RT-2.9 (Doxofylline) and RT-4.8 (Aciddegradation product) and m/e-149. The acid degradationproduct and pure drug were identified by LC-MS/MS inorder to establish acid degradation pathway (fig 5 and 6).
DOI: 10.6084/m9.figshare.3381295
title: Method development and hydrolytic degradation study of doxofylline by rp-hplc and lc-ms/ms

description: A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and photolyticdegradation product. The method showed a linear response for concentrations in the range of 1-200 g/ml usingacetonitrile: formic acid (90: 10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/min and retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness,robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. The hydrolytic degradation product as well as pathway was characterized by LC-MS/MS.RESULT AND DISCUSSION:A sensitive, selective, precise and accurate highperformance liquid chromatographic method of analysis ofdoxofylline in both as bulk drug and in formulation wasdeveloped and validated. The mobile phase consisted ofacetonitril: 0.05M formic Acid (90: 10v/v); pH-3.0. Thedetection wavelength was 274nm. This system was found to give the sharp peak for doxofylline (RT-2.9). The methodwas validated as per ICH guideline (table 1). Stabilityindicating assay method in which hydrolytic stresscondition was used for quantitative estimation ofdoxofylline in tablet formulation and identification ofhydrolytic degradation product The separation of drugfrom its degradation product were optimized by varying theratio &/or nature of organic modifier. Finally method wasdeveloped using same mobile phase composed ofacetonitrile: formic acid (90: 10); pH-3.0, in that both drugand degradation product showing good elution RT-2.9(Doxofylline) and RT-3.68 (Hydrolytic degradationproduct) and m/e-229 (fig 4). The hydrolytic degradationproduct and pure drug were identified by LC-MS/MS inorder to establish hydrolytic degradation pathway (fig 5 and6).<br>
DOI: 10.6084/m9.figshare.3381292
title: An analytical approach of doxofylline: review

description: Doxofylline is chemically designated as 7-(1, 3 dioxolone-2-yl methyl) theophylline. Presence of a dioxolane group inposition C-7 differentiates it from theophylline. It is a new antibronchospastic drug recently introduced in therapy withpharmacological properties like theophylline; a potent adenosine receptor antagonist. doxofylline do not affect gastric acid secretion; either invivo or in-vitro; unlike theophylline. The lack of side effects with doxofylline indicates that the drug can be used safely and effectively. Some analytical methods for quantitative determination of doxofylline in pharmaceutical formulations like UV-Spectrophotometry, HPLC and LC-MS are reported. The present review deals with the various analytical methods reported as well as adopted for the estimation of doxofylline.CONCLUSION:Doxofylline is available and frequently used in market;hence, there is great need to determine it in bulk, dosageform as well as to estabilish pharmacokinetic parameters.The need of analytical method validation is well reflect andhighlights the aspect of analyst’s personal habits, that frompart of the developed methods, and on the possibility of aperson getting very reproducible results when he carries outthe analysis again and again in a similar set of conditions.Any developed method however, may be influenced byvariable like different elapsed assay times, different days,reagent lots, instruments, equipments or apparatus,environmental condition like temperature etc.
DOI: 10.6084/m9.figshare.3381292.v1
title: An analytical approach of doxofylline: review

description: Doxofylline is chemically designated as 7-(1, 3 dioxolone-2-yl methyl) theophylline. Presence of a dioxolane group inposition C-7 differentiates it from theophylline. It is a new antibronchospastic drug recently introduced in therapy withpharmacological properties like theophylline; a potent adenosine receptor antagonist. doxofylline do not affect gastric acid secretion; either invivo or in-vitro; unlike theophylline. The lack of side effects with doxofylline indicates that the drug can be used safely and effectively. Some analytical methods for quantitative determination of doxofylline in pharmaceutical formulations like UV-Spectrophotometry, HPLC and LC-MS are reported. The present review deals with the various analytical methods reported as well as adopted for the estimation of doxofylline.CONCLUSION:Doxofylline is available and frequently used in market;hence, there is great need to determine it in bulk, dosageform as well as to estabilish pharmacokinetic parameters.The need of analytical method validation is well reflect andhighlights the aspect of analyst’s personal habits, that frompart of the developed methods, and on the possibility of aperson getting very reproducible results when he carries outthe analysis again and again in a similar set of conditions.Any developed method however, may be influenced byvariable like different elapsed assay times, different days,reagent lots, instruments, equipments or apparatus,environmental condition like temperature etc.
DOI: 10.6084/m9.figshare.3381286
title: Development and study of wound healing activity of an ayurvedic formulation

description: The effect of prepared Ayurvedic formulation was evaluated on excision and incision wound models in rats. The wound-healing activity was assessed by the rate of period of epithelialization and skin-breaking strength. Histologicalstudy of the granulation tissue was carried out to know the extent of collagen formation in the wound tissue. TheAyurvedic formulation prepared was then promoted for wound-healing activity in two wound models. The treatedanimals showed significant reduction in the wound area up to (P&lt;0.001) and faster rate of epithialisation (23.17±0.54).In an incision wound model, formulation treated animals demonstrated significant skin-breaking strength up to420.33±5.92. Histological studies of the tissue obtained from the formulation treated group revealed that the activitywas more significant in this group. Our present study reveals that the Ayurvedic formulation posses potent woundhealing activity, which could be a good choice of remedy for wound healing but less potent than standard Nitrofurazone.<br>
DOI: 10.6084/m9.figshare.3381286.v1
title: Development and study of wound healing activity of an ayurvedic formulation

description: The effect of prepared Ayurvedic formulation was evaluated on excision and incision wound models in rats. The wound-healing activity was assessed by the rate of period of epithelialization and skin-breaking strength. Histologicalstudy of the granulation tissue was carried out to know the extent of collagen formation in the wound tissue. TheAyurvedic formulation prepared was then promoted for wound-healing activity in two wound models. The treatedanimals showed significant reduction in the wound area up to (P&lt;0.001) and faster rate of epithialisation (23.17±0.54).In an incision wound model, formulation treated animals demonstrated significant skin-breaking strength up to420.33±5.92. Histological studies of the tissue obtained from the formulation treated group revealed that the activitywas more significant in this group. Our present study reveals that the Ayurvedic formulation posses potent woundhealing activity, which could be a good choice of remedy for wound healing but less potent than standard Nitrofurazone.<br>
DOI: 10.6084/m9.figshare.3381283
title: Development of novel hptlc method for estimation of qurcetin in Ocimum sanctum

description: HPTLC is a modern chromatographic technique in which the principle of TLC is sophisticated and automated bywhich the samples are accurately and precisely estimated which can be utilized for both qualitative and quantitativepurpose.Ocimum genus contains approximately 30 species of herbs and shrubs from the tropical and subtropical region of Asia,Africa and central and South America. However, the major place of diversity appears to be in Africa. The drug hasnumerous pharmacological activities like hypoglycemic, immunomodulatory, antistress, analgesic, antipyretic, antiinflammatory, antiulcerogenic, radio protective and antitumor. Major constituents of Ocimum sanctum includeseugenol, qurcetine etc. which are known to possess antifungal activity against candida albicans. The aim of the present study is to develop a novel High Performance liquid chromatographic (HPTLC) method for the quantitative estimation of flavonoid Qurcetine in hydroalcoholic extract of Ocimum sanctum.RESULT AND DISCUSSION:Comparing Rf values of standard and crude alcoholic extract following curves were obtained that were when compared and quantity of qurcetine was calculated it was found out to be 0.74 mg/ml. The method described above presents a novel solvent system for qualitative and quantitative estimation of qurcetine in Ocimum sanctum.CONCLUSION:In the quest for new antifungal drugs, medicinal plants mustnot be overlooked. Many essential oils possess antifungal or antibacterial activity and have been proposed for use incomplementary medicine. Although they are not widelyused in conventional health care, these oils have beenrecommended for use as home remedies for the treatment of vaginal candidiasis by numerous books and articles in the popular place.HPTLC is a modern chromatographic technique in whichthe principle of TLC is automated by which the samples areaccurately and precisely estimated which can be utilized forboth qualitative and quantitative purpose. In the presentinvestigation a novel solvent system for HPTLC analysis ofOcimum sanctum was designed which provides asophisticated method for determination of qurcetine inalcoholic extract of Ocimum sanctum, and thus can be used for quantitative studies.
DOI: 10.6084/m9.figshare.3381283.v1
title: Development of novel hptlc method for estimation of qurcetin in Ocimum sanctum

description: HPTLC is a modern chromatographic technique in which the principle of TLC is sophisticated and automated bywhich the samples are accurately and precisely estimated which can be utilized for both qualitative and quantitativepurpose.Ocimum genus contains approximately 30 species of herbs and shrubs from the tropical and subtropical region of Asia,Africa and central and South America. However, the major place of diversity appears to be in Africa. The drug hasnumerous pharmacological activities like hypoglycemic, immunomodulatory, antistress, analgesic, antipyretic, antiinflammatory, antiulcerogenic, radio protective and antitumor. Major constituents of Ocimum sanctum includeseugenol, qurcetine etc. which are known to possess antifungal activity against candida albicans. The aim of the present study is to develop a novel High Performance liquid chromatographic (HPTLC) method for the quantitative estimation of flavonoid Qurcetine in hydroalcoholic extract of Ocimum sanctum.RESULT AND DISCUSSION:Comparing Rf values of standard and crude alcoholic extract following curves were obtained that were when compared and quantity of qurcetine was calculated it was found out to be 0.74 mg/ml. The method described above presents a novel solvent system for qualitative and quantitative estimation of qurcetine in Ocimum sanctum.CONCLUSION:In the quest for new antifungal drugs, medicinal plants mustnot be overlooked. Many essential oils possess antifungal or antibacterial activity and have been proposed for use incomplementary medicine. Although they are not widelyused in conventional health care, these oils have beenrecommended for use as home remedies for the treatment of vaginal candidiasis by numerous books and articles in the popular place.HPTLC is a modern chromatographic technique in whichthe principle of TLC is automated by which the samples areaccurately and precisely estimated which can be utilized forboth qualitative and quantitative purpose. In the presentinvestigation a novel solvent system for HPTLC analysis ofOcimum sanctum was designed which provides asophisticated method for determination of qurcetine inalcoholic extract of Ocimum sanctum, and thus can be used for quantitative studies.
DOI: 10.6084/m9.figshare.3381280
title: Spectrometric method for simultaneous estimation of nimesulide and diclofenac sodium in pharmaceutical dosage form

description: A simple accurate spectrophotometric method has been developed for simultaneous estimation of nimesulide anddiclofenac sodium. The method involves solving of simultaneous equation based on measurement of absorbance at two wavelengths i.e., at 276 nm and 395 nm.RESULTS AND DISCUSSION:The proposed method for simultaneous estimation ofnimesulide and diclofenac sodium in combined dosageforms was found to be simple, accurate, economical andrapid. The values of coefficient of variation weresatisfactory low and recoveries were closed to 100% forboth the drugs.The method employs solving of simultaneous equations.Once the equations are determined analysis requires onlythe measuring of the absorbance of the sample solution atthe two wavelengths selected, followed by a few simplecalculations.The proposed method is fast accurate and precise for thedetermination of nimesulide and diclofenac sodium incombined soft capsule formulations. Hence, it can beemployed for routine analysis in Quality ControlLaboratories.<br><br><br><br>
DOI: 10.6084/m9.figshare.3381280.v1
title: Spectrometric method for simultaneous estimation of nimesulide and diclofenac sodium in pharmaceutical dosage form

description: A simple accurate spectrophotometric method has been developed for simultaneous estimation of nimesulide anddiclofenac sodium. The method involves solving of simultaneous equation based on measurement of absorbance at two wavelengths i.e., at 276 nm and 395 nm.RESULTS AND DISCUSSION:The proposed method for simultaneous estimation ofnimesulide and diclofenac sodium in combined dosageforms was found to be simple, accurate, economical andrapid. The values of coefficient of variation weresatisfactory low and recoveries were closed to 100% forboth the drugs.The method employs solving of simultaneous equations.Once the equations are determined analysis requires onlythe measuring of the absorbance of the sample solution atthe two wavelengths selected, followed by a few simplecalculations.The proposed method is fast accurate and precise for thedetermination of nimesulide and diclofenac sodium incombined soft capsule formulations. Hence, it can beemployed for routine analysis in Quality ControlLaboratories.<br><br><br><br>
DOI: 10.6084/m9.figshare.3381277
title: Mucoadhesive polymers: means of improving the mucoadhesive properties of drug delivery system

description: The goal of any drug delivery system is to provide a therapeutic amount of drug to the proper site in the body to achieve promptly & then maintain the desired drug concentration. That is why the drug delivery system should deliver dr ug at a state dictated by the needs of the body over a specified period of treatment. This idealized objective points to the two aspects most important to drug delivery, namely, spatial placement relates to targeting a drug to a specific organ or tissue while temporal delivery refers to the control of rate of drug delivery to the target tissue.Bioadhesion can be defined as the process by which a natural or a synthetic polymer can adhere to a biological substrate. When the biological substrate is a mucosal layer then the phenomena is known as mucoadhesion. The substrate possessing bioadhesive property can help in devising a delivery system capable of delivering a bioactive agent for a prolonged period of time at a specific delivery site. The current review provides a good insight on mucoadhesive polymers, the phenomenon of mucoadhesion and the factors which have the ability to affect the mucoadhesive properties of a polymer.
DOI: 10.6084/m9.figshare.3381277.v2
title: Mucoadhesive polymers: means of improving the mucoadhesive properties of drug delivery system

description: The goal of any drug delivery system is to provide a therapeutic amount of drug to the proper site in the body to achieve promptly & then maintain the desired drug concentration. That is why the drug delivery system should deliver dr ug at a state dictated by the needs of the body over a specified period of treatment. This idealized objective points to the two aspects most important to drug delivery, namely, spatial placement relates to targeting a drug to a specific organ or tissue while temporal delivery refers to the control of rate of drug delivery to the target tissue.Bioadhesion can be defined as the process by which a natural or a synthetic polymer can adhere to a biological substrate. When the biological substrate is a mucosal layer then the phenomena is known as mucoadhesion. The substrate possessing bioadhesive property can help in devising a delivery system capable of delivering a bioactive agent for a prolonged period of time at a specific delivery site. The current review provides a good insight on mucoadhesive polymers, the phenomenon of mucoadhesion and the factors which have the ability to affect the mucoadhesive properties of a polymer.
DOI: 10.6084/m9.figshare.3381277.v1
title: mucoadhesive final.pdf

description: The goal of any drug delivery system is to provide a therapeutic amount of drug to the proper site in the body to achieve promptly & then maintain the desired drug concentration. That is why the drug delivery system should deliver dr ug at a state dictated by the needs of the body over a specified period of treatment. This idealized objective points to the two aspects most important to drug delivery, namely, spatial placement relates to targeting a drug to a specific organ or tissue while temporal delivery refers to the control of rate of drug delivery to the target tissue.Bioadhesion can be defined as the process by which a natural or a synthetic polymer can adhere to a biological substrate. When the biological substrate is a mucosal layer then the phenomena is known as mucoadhesion. The substrate possessing bioadhesive property can help in devising a delivery system capable of delivering a bioactive agent for a prolonged period of time at a specific delivery site. The current review provides a good insight on mucoadhesive polymers, the phenomenon of mucoadhesion and the factors which have the ability to affect the mucoadhesive properties of a polymer.