cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/xwsn6-zdy70
title: E. coli CusCFBA-CusC-K98H

description: Overexpressed CusCFBA efflux pump in E. coli C43 (DE3).Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-06-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Edward Yu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-19/rawdata/</p>CusCFBA_CusCK98H_20016.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-19/rawdata/CusCFBA_CusCK98H_20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        CusCFBA_CusCK98H_20016_part121_38.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j1chp-djb08
title: Hyphomonas AR46

description: ParA-like protein, HNE0708 knockout
description: <strong>Tilt Series Date:</strong> 2012-09-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in Marine Broth (MB) medium at 30 degrees without antibiotics. OD600=0.6</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2012-09-07-19_slicer7052.jpg: REZ ycw2012-09-07-19_slicer7057.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-07-19/rawdata/</p>20120907_AR46_10019.mrc,  Tilt Series (Pixel Size 1.0 nm), 925.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-07-19/rawdata/20120907_AR46_10019.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120907_AR46_10019_part132_2.rec,  Reconstruction (Pixel Size 2.0 nm), 262.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-07-19/3dimage_20701/20120907_AR46_10019_part132_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ag4sy-tj112
title: Myxococcus xanthus delta-pilQ

description: grid box YWC061
description: <strong>Tilt Series Date:</strong> 2013-12-30</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-47/rawdata/</p>20131230_Janelia_YWC061_10038.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-47/rawdata/20131230_Janelia_YWC061_10038.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131230_Janelia_YWC061_10038.rec,  Reconstruction (Pixel Size 1.68 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-47/3dimage_37285/20131230_Janelia_YWC061_10038.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pmxyt-9wp35
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-7/rawdata/</p>20131221_YWC063_10007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-7/rawdata/20131221_YWC063_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_10007_part131_2.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tp5e9-cw327
title: Myxococcus xanthus delta-pilC

description: grid box YWC060
description: <strong>Tilt Series Date:</strong> 2013-12-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-39/rawdata/</p>20131226_Janelia_YWC060_10025.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-39/rawdata/20131226_Janelia_YWC060_10025.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131226_Janelia_YWC060_10025_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jcr1f-c6z58
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC106
description: <strong>Tilt Series Date:</strong> 2014-07-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-55/rawdata/</p>20140707_YWC106_30007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-55/rawdata/20140707_YWC106_30007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140707_YWC106_30007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-55/3dimage_44485/20140707_YWC106_30007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.29992288
title: A novel multi-visit truck-drone collaborative optimization model for the green vehicle routing problem with dynamic flight endurance

description: This is the experimental data for the manuscript titled "A novel multi-visit truck-drone collaborative optimization model for the green vehicle routing problem with dynamic flight endurance".
DOI: 10.6084/m9.figshare.29992288.v1
title: A novel multi-visit truck-drone collaborative optimization model for the green vehicle routing problem with dynamic flight endurance

description: This is the experimental data for the manuscript titled "A novel multi-visit truck-drone collaborative optimization model for the green vehicle routing problem with dynamic flight endurance".
DOI: 10.15131/shef.data.29224874
title: Data and analysis for "From symbiosis to scarcity: Evaluating disruption associated with decarbonisation to circular waste materials between the UK cement and steel sectors"

description: This dataset contains the analysis for the research article entitled "From symbiosis to scarcity: Evaluating disruption associated with decarbonisation to circular waste materials between the UK cement and steel sectors".Dataset available as an interlinked XLSX spreadsheet or as individual CSV files, where: ReadME) Dataset metadata, 1) A material flow analysis on the cement and concrete sector and the steel sector in the UK, 2) A material flow analysis on the cement and concrete sector, and the iron and steel sector globally, 3) Calculating the current and future carbon intensity of steel per tonne of product utilising the UK and global material flow analyses, 4) Calculating the current and future carbon intensity of cement per tonne of product utilising the UK and global material flow analyses, 5) Calculating the average transportation distance by sea from China, India, and Japan to the UK from the three largest ports (by economic value) from each country to the UK's three largest ports (by economic value), 6) Assessment of the current, and prediction of the future economic value of iron, steel, and GGBS under both import and export scenarions using historical UK trade data (2000 onwards), 7) Supplementary calculations related to the steel sector to support other sheet, 8) Supplementary calculations related to the steel sector to support other sheets.
DOI: 10.15131/shef.data.29224874.v2
title: Data and analysis for "From symbiosis to scarcity: Evaluating disruption associated with decarbonisation to circular waste materials between the UK cement and steel sectors"

description: This dataset contains the analysis for the research article entitled "From symbiosis to scarcity: Evaluating disruption associated with decarbonisation to circular waste materials between the UK cement and steel sectors".Dataset available as an interlinked XLSX spreadsheet or as individual CSV files, where: ReadME) Dataset metadata, 1) A material flow analysis on the cement and concrete sector and the steel sector in the UK, 2) A material flow analysis on the cement and concrete sector, and the iron and steel sector globally, 3) Calculating the current and future carbon intensity of steel per tonne of product utilising the UK and global material flow analyses, 4) Calculating the current and future carbon intensity of cement per tonne of product utilising the UK and global material flow analyses, 5) Calculating the average transportation distance by sea from China, India, and Japan to the UK from the three largest ports (by economic value) from each country to the UK's three largest ports (by economic value), 6) Assessment of the current, and prediction of the future economic value of iron, steel, and GGBS under both import and export scenarions using historical UK trade data (2000 onwards), 7) Supplementary calculations related to the steel sector to support other sheet, 8) Supplementary calculations related to the steel sector to support other sheets.
DOI: 10.15131/shef.data.29224874.v1
title: Data and analysis for "From symbiosis to scarcity: Evaluating disruption associated with decarbonisation to circular waste materials between the UK cement and steel sectors"

description: This dataset contains the analysis for the research article entitled "From symbiosis to scarcity: Evaluating disruption associated with decarbonisation to circular waste materials between the UK cement and steel sectors".A clear README to familiarise the reader with the purpose and layout of the analysis can be found on "Sheet 1" of the dataset.
DOI: 10.22002/6wh8t-vjs81
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-09-09</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-09-09-8/rawdata/</p>tomography9.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-09-09-8/rawdata/tomography9.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography9_part121_1.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-09-09-8/3dimage_46194/tomography9_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tdmze-bne27
title: Myxococcus xanthus TM530

description: Wza0 knockoutGrid box YWC029
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Tam Mignot</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-19/rawdata/</p>20131025_YWC029_20001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-19/rawdata/20131025_YWC029_20001.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_YWC029_20001_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-19/3dimage_33372/20131025_YWC029_20001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7sjyp-as313
title: Hylemonella gracilis

description: flagellar motor
description: <strong>Tilt Series Date:</strong> 2012-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 112.0 eV/Å²,  defocus: -15.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2012-09-23-45_slicer4997.jpg: Nucleoid ycw2012-09-23-45_slicer4999.jpg: Extracellular vesicles ycw2012-09-23-45_slicer5000.jpg: Flagella ycw2012-09-23-45_slicer5003.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-45/rawdata/</p>20120923_Hylemonella_30009.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-45/rawdata/20120923_Hylemonella_30009.mrc"
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        20120923_Hylemonella_30009_full.rec,  Reconstruction (Pixel Size 1.96 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-45/3dimage_20942/20120923_Hylemonella_30009_full.rec"
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DOI: 10.22002/fzcze-64706
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-08-19_slicer7948.jpg: pili .  external vesicle .  Myxococcus xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-19/rawdata/</p>20140308_YWC093_10042.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-19/rawdata/20140308_YWC093_10042.mrc"
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        20140308_YWC093_10042_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
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