cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/e48e2-gng68
title: E. coli pED208

description: E. coli with plasmid pED208
description: <strong>Tilt Series Date:</strong> 2014-12-16</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells arrived dead.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-39/rawdata/</p>pED2080031.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-39/rawdata/pED2080031.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080031_part121_5.rec,  Reconstruction (Pixel Size 2.52 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-39/3dimage_48853/pED2080031_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/62x83-6vp55
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-6/rawdata/</p>12mar14_c121_1_Cos74ADN_AT1bt10010.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-6/rawdata/12mar14_c121_1_Cos74ADN_AT1bt10010.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar14_c121_1_Cos74ADN_AT1bt10010_part68_6.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-6/3dimage_17743/12mar14_c121_1_Cos74ADN_AT1bt10010_part68_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/75sgd-mj571
title: Agro C58 22C plate 16h

description: Raw data files of Agro C58 22C plate 16h
description: <strong>Tilt Series Date:</strong> 2014-11-10</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown at 22C and induced for T pili on a 2% agarose pad for 16 hours</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-64/rawdata/</p>c58_22_pl_16h90017.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-64/rawdata/c58_22_pl_16h90017.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_22_pl_16h90017_part121_49.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-10-64/3dimage_49586/c58_22_pl_16h90017_part121_49.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3aq6w-sgs35
title: HUVECs infx pNLGFP

description: Raw data files of HUVECs infx pNLGFP
description: <strong>Tilt Series Date:</strong> 2013-07-03</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen UCLA)</p>
<strong>Sample Preparation:</strong> Whole cells grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-03-48/rawdata/</p>13jul03_HUVECspnl24hPI_montage_33x33_0051.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-03-48/rawdata/13jul03_HUVECspnl24hPI_montage_33x33_0051.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jul03_HUVECspnl24hPI_montage_33x33_0051_full.rec,  Reconstruction (Pixel Size 2.24 nm), 602.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-03-48/3dimage_28000/13jul03_HUVECspnl24hPI_montage_33x33_0051_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/390xf-q6045
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-05-05</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-05-25/3dimage_69307/</p>12may05_C126_4_pnlDN_Cos7_AT7BT1_0005_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-05-25/3dimage_69307/12may05_C126_4_pnlDN_Cos7_AT7BT1_0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may05_C126_4_pnlDN_Cos7_AT7BT1_0005.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-05-25/rawdata/12may05_C126_4_pnlDN_Cos7_AT7BT1_0005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y1cdj-42k29
title: M-MuLV 43D infected MEFs

description: M-MuLV 43D wildtype
description: <strong>Tilt Series Date:</strong> 2014-04-21</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> M-MuLV infected cells provided by Hung Fan (UC Irvine)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole MEFs infected with M-MuLV 43D were cultured on Au grids, blotted manually and plunge frozen.</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-04-21-48/3dimage_41923/</p>14apr21_C181-2MLV43D_3t3_pos20_AT3BT1_0008_full.rec,  Reconstruction (Pixel Size 2.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-04-21-48/3dimage_41923/14apr21_C181-2MLV43D_3t3_pos20_AT3BT1_0008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        14apr21_C181-2MLV43D_3t3_pos20_AT3BT1_0008.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-04-21-48/rawdata/14apr21_C181-2MLV43D_3t3_pos20_AT3BT1_0008.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v4dnh-p7855
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-10/rawdata/</p>12mar14_c121_1_Cos74ADN_AT2bt10001.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-10/rawdata/12mar14_c121_1_Cos74ADN_AT2bt10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar14_c121_1_Cos74ADN_AT2bt10001_full.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-10/3dimage_17751/12mar14_c121_1_Cos74ADN_AT2bt10001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nrzhz-t1802
title: COS7 tfx pNL4GFP and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing pNLGFP and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-15</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> VPS4B (E235Q) expression construct provided by W.Sundquist (Utah); pNLGFP construct provided by I. Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-15-8/rawdata/</p>12mar15_c108_1_Cos7pNLDN_AT1bt10020.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-15-8/rawdata/12mar15_c108_1_Cos7pNLDN_AT1bt10020.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar15_c108_1_Cos7pNLDN_AT1bt10020_full.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-15-8/3dimage_17719/12mar15_c108_1_Cos7pNLDN_AT1bt10020_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y0nbn-2vr35
title: HUVECs infx pNLBg1I

description: Raw data files of HUVECs infx pNLBg1I
description: <strong>Tilt Series Date:</strong> 2013-10-19</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen endpoint of virus infected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-27/rawdata/</p>13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0012.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-27/rawdata/13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0012_part121_62.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ujytb3
title: Occurrence Download

description: A dataset containing 36 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chlorophytum comosum",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 36 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047707-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.npw896
title: Occurrence Download

description: A dataset containing 712 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Argemone mexicana"
 ]
}

 The dataset includes 712 records from 77 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047706-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/3tbce-vw867
title: HeLa

description: Synchronized, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2014-01-06</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> With Doug Mckay and Katherine Ullman</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA NUP 153+ Thymidine 2mM. 14 hour after release</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-46/rawdata/</p>midbody10026.mrc,  Tilt Series (Pixel Size 0.6 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-46/rawdata/midbody10026.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody10026.rec,  Reconstruction (Pixel Size 2.4 nm), 310.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-46/3dimage_50465/midbody10026.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wr7f5-wsr22
title: COS7 pNLGFP

description: Cos7 cells transfected with pNLGFP provirus construct. Sample #C112
description: <strong>Tilt Series Date:</strong> 2012-05-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected using lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-14-1/3dimage_69311/</p>12may14_C112_2_pNLonly_AT1MA1_full.rec,  Reconstruction (Pixel Size 2.0 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-14-1/3dimage_69311/12may14_C112_2_pNLonly_AT1MA1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may14_C112_2_pNLonly_AT1MA1.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5c5a6-0yp86
title: Agro_C58

description: Agro_liq_pl_25C
description: <strong>Tilt Series Date:</strong> 2014-09-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AB media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-09-11-10/rawdata/</p>t230001.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.1 GB  
        
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        18_full.rec,  Reconstruction (Pixel Size 2.0 nm), 798.2 MB  
        
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DOI: 10.22002/x52s1-ca958
title: Agro pJW58

description: wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter.  Kanamycin-resistant (150 to 300 ug/mL)
description: <strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-15/rawdata/</p>4_pJW58_20008.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
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        4_pJW58_20008_part121_14.rec,  Reconstruction (Pixel Size 1.6 nm), 356.4 MB  
        
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