cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/vn4tj-w4s69
title: INS-1E with Calreticulin-YFP

description: CLEM and ECT
description: <strong>Tilt Series Date:</strong> 2016-04-17</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 0.47368421 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zachary Freyberg from University of Pittsburgh provided the frozen cells on grids. Collaboration via Stephen Carter. CLEM and ECT done by me.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-12/rawdata/</p>Calreticulin-TS3.st,  Tilt Series (Pixel Size 0.6 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-12/rawdata/Calreticulin-TS3.st"
        > <i class="download icon"></i> Download </a></p>

        Calreticulin-TS3_full.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-12/3dimage_74341/Calreticulin-TS3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.647fbu
title: Occurrence Download

description: A dataset containing 138 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pseudotsuga menziesii",
 "Country is Norway",
 "HasCoordinate is true"
 ]
}

 The dataset includes 138 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047484-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/k6sds-9ks77
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-36/3dimage_71468/</p>atlas1_sq40039_full.rec,  Reconstruction (Pixel Size 1.28 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-36/3dimage_71468/atlas1_sq40039_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40039.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-36/rawdata/atlas1_sq40039.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m4wq0-ryn75
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-486/rawdata/</p>T4SSWTminiNov21A021_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-486/rawdata/T4SSWTminiNov21A021_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTminiNov21A021_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-486/3dimage_105213/T4SSWTminiNov21A021_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z3fr9-m5a31
title: wt_serum_blotfrombehind

description: This is a sample of wt grown in serum bottle that made nanowires.
description: <strong>Tilt Series Date:</strong> 2015-07-26</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Sample Preparation:</strong> The sample was put on the C side (glow discharged side) on the left. The blotting was done from behind on the rt side. Vitrobot was used. All other vitrobot conditions are the same.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-23/rawdata/</p>10023.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-23/rawdata/10023.mrc"
        > <i class="download icon"></i> Download </a></p>

        10023.rec,  Reconstruction (Pixel Size 1.92 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-23/3dimage_59598/10023.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.23ueja
title: Occurrence Download

description: A dataset containing 2781 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pseudotsuga menziesii",
 "Country is New Zealand",
 "HasCoordinate is true"
 ]
}

 The dataset includes 2781 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047482-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.k6mvyc
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Argusianus argus",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047483-250811113504898/datasets/export for details.
DOI: 10.22002/26zzd-w0505
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-70/rawdata/</p>LP02May18B016_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-70/rawdata/LP02May18B016_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        LP02May18B016_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-70/3dimage_104876/LP02May18B016_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hz2eh-8px11
title: TopJ + MPN119

description: Raw data files of TopJ + MPN119
description: <strong>Tilt Series Date:</strong> 2016-06-06</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Mycoplasma pneumoniae</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Edward Sheppard from Duncan Krause's lab prepared the cell culture</p>
  scy2016-06-06-4_slicer16918.jpg: attachment organelle scy2016-06-06-4_slicer16919.jpg: attachment organelle scy2016-06-06-4_slicer16920.jpg: attachment organelle end region
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-06-06-4/rawdata/</p>at90004.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-06-06-4/rawdata/at90004.mrc"
        > <i class="download icon"></i> Download </a></p>

        at90004_part131_3.rec,  Reconstruction (Pixel Size 1.56 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-06-06-4/3dimage_75408/at90004_part131_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.5vnhj9
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pungitius hellenicus",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Geometry POLYGON ((-6.6 29.26705, -6.6 50.94, 40.81373 50.94, 40.81373 29.26705, -6.6 29.26705))",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Observation, Material sample)"
 ]
}

 The dataset includes 3 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047485-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/55dgv-bz817
title: NR1769/R1-16 conjugation

description: Grid box YWC241
description: <strong>Tilt Series Date:</strong> 2017-01-30</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
  ycw2017-01-30-30_slicer17677.jpg: T4SS machine ycw2017-01-30-30_slicer17678.jpg: MS2 phage bound to F pili
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-01-30-30/rawdata/</p>20170130_YWC241_10030.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-01-30-30/rawdata/20170130_YWC241_10030.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170130_YWC241_10030_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-01-30-30/3dimage_81659/20170130_YWC241_10030_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.u755vm
title: Occurrence Download

description: A dataset containing 19994 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Myosotis secunda"
 ]
}

 The dataset includes 19994 records from 225 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047481-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/qjqwn-zzw06
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-16/3dimage_71429/</p>atlas1_sq40019.rec,  Reconstruction (Pixel Size 1.28 nm), 711.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-16/3dimage_71429/atlas1_sq40019.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40019.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-16/rawdata/atlas1_sq40019.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ktphg-f2875
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-13</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-13-3/3dimage_71476/</p>atlas1_sq40043_mcorr_part111_3.rec,  Reconstruction (Pixel Size 1.28 nm), 267.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-13-3/3dimage_71476/atlas1_sq40043_mcorr_part111_3.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40043_mcorr.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-13-3/rawdata/atlas1_sq40043_mcorr.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d8vka-p0896
title: Shewanella oneidensis dFlgH

description: Raw data files of Shewanella oneidensis dFlgH
description: <strong>Tilt Series Date:</strong> 2018-05-27</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Jeff Granlick (Uni. of Minnesota) supplied the mutant cells.</p>
<strong>Sample Preparation:</strong> Samples were prepared from overnight LB culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-45/rawdata/</p>tilt50.mrc,  Tilt Series (Pixel Size 0.57 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-45/rawdata/tilt50.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt50_part101_3.rec,  Reconstruction (Pixel Size 2.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-45/3dimage_93382/tilt50_part101_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bx1st-aw353
title: Legionella delta DotOB

description: DotF-GFP background
description: <strong>Tilt Series Date:</strong> 2019-02-13</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph Vogel</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase</p>
<strong>Sample Preparation:</strong> 10 nm gold beads, broad vitrobot, R2/2 200 mesh copper quantifoil grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-45/rawdata/</p>20190213_deltaOB_28.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-45/rawdata/20190213_deltaOB_28.mrc"
        > <i class="download icon"></i> Download </a></p>

        20190213_deltaOB_28_full.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-45/3dimage_101472/20190213_deltaOB_28_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g2bwq-fzt16
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-33/rawdata/</p>caulobacter_na1000_wt_034_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-33/rawdata/caulobacter_na1000_wt_034_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_034_af.mrc.mdoc,  813.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-33/rawdata/caulobacter_na1000_wt_034_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>