cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11313992
title: Foliar application of zinc in the agronomic biofortification of arugula

description: Abstract Zinc deficiency affects one third of the population and a possible solution is the agronomic biofortification of vegetables. Due to the increased consumption of arugula, this vegetable has good potential of use; however, there are few researches on the subject. The objective of this study was to evaluate the effect of foliar zinc applications, at different times, on physiological, yield, nutritional, and biofortification parameters of the ‘Broad Leaf’ arugula. Three doses (0.5, 1, and 1.5 kg ha-1 Zn) were evaluated at five times (15; 20; 25; 15 and 20; and 15, 20 and 25 days after emergence) and a control treatment. It was observed that there was no effect of the application on the physiological variables, as well as on the height, leaf area, and fresh mass of aerial parts of the plant. Zn sprayings affected the N contents, while for Mn, P, Cu, and Fe, no significant effects were found. It was verified that with the application of 1.5 kg ha-1 Zn made at 25 days after emergence, a higher Zn content was obtained in the arugula leaves, representing an increase of 279% in relation to the control, showing the effectiveness of foliar zinc applications as an agronomic biofortification strategy for arugula.
DOI: 10.6084/m9.figshare.11313995
title: Optimization of ultrasound-assisted extraction of bioactive compounds from jabuticaba (Myrciaria cauliflora) fruit through a Box-Behnken experimental design

description: Abstract Jabuticaba is a very popular fruit in Brazil being a great source of compounds with considerable biological activities. Novel optimized ultrasound-assisted extraction (UAE) methods have been proposed for anthocyanins and total phenolic compounds from jabuticaba. A Box–Behnken experimental design (BBD) with a response surface methodology (RSM) was used to investigate the effect of six independent variables (solvent composition, solvent-to-sample ratio, ultrasound amplitude and cycle, pH, and temperature) on the UAE. Solvent composition was found to be the most significant variable for the extraction of both anthocyanins (51%) and total phenolic compounds (72%). The other optimum conditions for anthocyanins were as follows: pH 7.00, 39.8 ºC, 20:1.5 mL:g solvent-to-sample ratio, 34% ultrasound amplitude and cycle of 0.47 seconds. The optimum conditions for the extraction of phenolic compounds were: pH 7.00, 26.0 ºC, 20:1.5 (mL:g) solvent-to-sample ratio, 68.5% ultrasound amplitude and cycle of 0.5 seconds. The extraction kinetic was also evaluated. The developed methods showed a high precision, with coefficients of variation of less than 5% for both repeatability and intermediate precision (within-lab reproducibility). The applicability of the new methods was successfully evaluated on several fruits and jams from jabuticaba.
DOI: 10.6084/m9.figshare.11313989
title: Language teaching: a comparison of social representations held by first- and fifth-year students of the Translation Studies undergraduate programme at Universidad de Santiago de Chile

description: ABSTRACT This article compares the social representations about the processes of teaching and learning a foreign language held by first- and fifth-year students of the translation studies undergraduate programme at Universidad de Santiago de Chile (USACH). The concept of social representations is taken from social psychology (Jodelet 1988, 2008; Moscovici 1979).A word association task and a semi structured interview were administered to four groups of translator trainees. Results show significant changes in terms of language proficiency, teaching and learning. It is concluded that such changes would promote the adoption of new methodologies for the foreign language course units in this translation programme.
DOI: 10.6084/m9.figshare.11313995.v1
title: Optimization of ultrasound-assisted extraction of bioactive compounds from jabuticaba (Myrciaria cauliflora) fruit through a Box-Behnken experimental design

description: Abstract Jabuticaba is a very popular fruit in Brazil being a great source of compounds with considerable biological activities. Novel optimized ultrasound-assisted extraction (UAE) methods have been proposed for anthocyanins and total phenolic compounds from jabuticaba. A Box–Behnken experimental design (BBD) with a response surface methodology (RSM) was used to investigate the effect of six independent variables (solvent composition, solvent-to-sample ratio, ultrasound amplitude and cycle, pH, and temperature) on the UAE. Solvent composition was found to be the most significant variable for the extraction of both anthocyanins (51%) and total phenolic compounds (72%). The other optimum conditions for anthocyanins were as follows: pH 7.00, 39.8 ºC, 20:1.5 mL:g solvent-to-sample ratio, 34% ultrasound amplitude and cycle of 0.47 seconds. The optimum conditions for the extraction of phenolic compounds were: pH 7.00, 26.0 ºC, 20:1.5 (mL:g) solvent-to-sample ratio, 68.5% ultrasound amplitude and cycle of 0.5 seconds. The extraction kinetic was also evaluated. The developed methods showed a high precision, with coefficients of variation of less than 5% for both repeatability and intermediate precision (within-lab reproducibility). The applicability of the new methods was successfully evaluated on several fruits and jams from jabuticaba.
DOI: 10.6084/m9.figshare.11313998.v1
title: There is always something to teach, there is always something to learn: how and why should we teach mathematics to senior people?

description: Abstract This paper is related to the development of educative actions with senior people. More specifically, it refers to the Mathematical Education promoted in the space of university extension activities. It is considered that the engagement of senior people in educational environments can contribute to the maintenance and development of cognitive aspects, which are fundamental to their autonomy. In addition, participation in these environments can favor changes in their physical, social, and psychological aspects, enhancing their self-esteem. In this paper, we discuss results of a research which aimed at understanding possible ways to promote such educational actions and to providing elements to a reflection about its contributions to senior people. Data production occurred within the project Conversation about Mathematics with Senior People. Data were collect through interviews, photos, and notes in a research notebook. Data analysis reveals an active engagement of the women and men in the project as well as provides some indication about contributions to the senior people. About their engagement, we considered: a) their interest in discussing the subjects during the meetings; b) their persistence to perform mathematics tasks on their own; c) their argumentation about the ideas showed; and d) their movement of sharing the activities with other people who were not participating in the project. In relation to contribution to senior people, we considered manifestations that expressed: a) improvement of cognitive aspects; b) opportunity for social interaction; and c) the possibility of learning new subjects related to Mathematics.
DOI: 10.6084/m9.figshare.11314001.v1
title: Diosmin induces caspase-dependent apoptosis in human glioblastoma cells

description: Abstract: Diosmin is a flavone glycoside clinically used as the main component of Daflon for the treatment of venous diseases. Several studies demonstrated that this natural compound can induce apoptosis in different tumors. However, isolated diosmin has not been studied regarding its effects on glioblastoma so far. Since glioblastoma is a highly lethal and fast-growing brain tumor, new therapeutic strategies are urgently needed. Herein, we evaluated the role of this flavonoid against glioblastoma cells using in vitro assays. Diosmin significantly reduced the viability of GBM95, GBM02, and U87MG glioblastoma cells, but not of healthy human astrocytes, as verified by MTT assay. Vimentin immunostaining showed that diosmin induced morphological changes in GBM95 and GBM02 cells, making them smaller and more polygonal. Diosmin did not inhibit GBM95 and GBM02 cell proliferation, but it caused DNA fragmentation, as verified by the TUNEL assay, and increased cleaved caspase-3 expression in these cells. In summary, diosmin is able to induce caspase-dependent apoptosis specifically in tumor cells and, therefore, could be considered a promising therapeutic compound against glioblastoma.
DOI: 10.6084/m9.figshare.11313983
title: Environmental Enrichment Promotes Antioxidant Effect in the Ventrolateral Medulla and Kidney of Renovascular Hypertensive Rats

description: Abstract Background: Arterial hypertension is a precursor to the development of heart and renal failure, furthermore is associated with elevated oxidative markers. Environmental enrichment of rodents increases performance in memory tasks, also appears to exert an antioxidant effect in the hippocampus of normotensive rats. Objectives: Evaluate the effect of environmental enrichment on oxidative stress in the ventrolateral medulla, heart, and kidneys of renovascular hypertensive rats. Methods: Forty male Fischer rats (6 weeks old) were divided into four groups: normotensive standard condition (Sham-St), normotensive enriched environment (Sham-EE), hypertensive standard condition (2K1C-St), and hypertensive enriched environment (2K1C-EE). Animals were kept in enriched or standard cages for four weeks after all animals were euthanized. The level of significance was at p &lt; 0.05. Results: 2K1C-St group presented higher mean arterial pressure (mmHg) 147.0 (122.0; 187.0) compared to Sham-St 101.0 (94.0; 109.0) and Sham-EE 106.0 (90.8; 117.8). Ventrolateral medulla from 2K1C-EE had higher superoxide dismutase (SOD) (49.1 ± 7.9 U/mg ptn) and catalase activity (0.8 ± 0.4 U/mg ptn) compared to SOD (24.1 ± 9.8 U/mg ptn) and catalase activity (0.3 ± 0.1 U/mg ptn) in 2K1C-St. 2K1C-EE presented lower lipid oxidation (0.39 ± 0.06 nmol/mg ptn) than 2K1C-St (0.53 ± 0.22 nmol/mg ptn) in ventrolateral medulla. Furthermore, the kidneys of 2K1C-EE (11.9 ± 2.3 U/mg ptn) animals presented higher superoxide-dismutase activity than those of 2K1C-St animals (9.1 ± 2.3 U/mg ptn). Conclusion: Environmental enrichment induced an antioxidant effect in the ventrolateral medulla and kidneys that contributes to reducing oxidative damage among hypertensive rats.
DOI: 10.6084/m9.figshare.11313986
title: Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle

description: ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A&gt;G (CAST/DdeI) and AF248054.2:g.6545C&gt;T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels.
DOI: 10.6084/m9.figshare.11313992.v1
title: Foliar application of zinc in the agronomic biofortification of arugula

description: Abstract Zinc deficiency affects one third of the population and a possible solution is the agronomic biofortification of vegetables. Due to the increased consumption of arugula, this vegetable has good potential of use; however, there are few researches on the subject. The objective of this study was to evaluate the effect of foliar zinc applications, at different times, on physiological, yield, nutritional, and biofortification parameters of the ‘Broad Leaf’ arugula. Three doses (0.5, 1, and 1.5 kg ha-1 Zn) were evaluated at five times (15; 20; 25; 15 and 20; and 15, 20 and 25 days after emergence) and a control treatment. It was observed that there was no effect of the application on the physiological variables, as well as on the height, leaf area, and fresh mass of aerial parts of the plant. Zn sprayings affected the N contents, while for Mn, P, Cu, and Fe, no significant effects were found. It was verified that with the application of 1.5 kg ha-1 Zn made at 25 days after emergence, a higher Zn content was obtained in the arugula leaves, representing an increase of 279% in relation to the control, showing the effectiveness of foliar zinc applications as an agronomic biofortification strategy for arugula.
DOI: 10.6084/m9.figshare.11313983.v1
title: Environmental Enrichment Promotes Antioxidant Effect in the Ventrolateral Medulla and Kidney of Renovascular Hypertensive Rats

description: Abstract Background: Arterial hypertension is a precursor to the development of heart and renal failure, furthermore is associated with elevated oxidative markers. Environmental enrichment of rodents increases performance in memory tasks, also appears to exert an antioxidant effect in the hippocampus of normotensive rats. Objectives: Evaluate the effect of environmental enrichment on oxidative stress in the ventrolateral medulla, heart, and kidneys of renovascular hypertensive rats. Methods: Forty male Fischer rats (6 weeks old) were divided into four groups: normotensive standard condition (Sham-St), normotensive enriched environment (Sham-EE), hypertensive standard condition (2K1C-St), and hypertensive enriched environment (2K1C-EE). Animals were kept in enriched or standard cages for four weeks after all animals were euthanized. The level of significance was at p &lt; 0.05. Results: 2K1C-St group presented higher mean arterial pressure (mmHg) 147.0 (122.0; 187.0) compared to Sham-St 101.0 (94.0; 109.0) and Sham-EE 106.0 (90.8; 117.8). Ventrolateral medulla from 2K1C-EE had higher superoxide dismutase (SOD) (49.1 ± 7.9 U/mg ptn) and catalase activity (0.8 ± 0.4 U/mg ptn) compared to SOD (24.1 ± 9.8 U/mg ptn) and catalase activity (0.3 ± 0.1 U/mg ptn) in 2K1C-St. 2K1C-EE presented lower lipid oxidation (0.39 ± 0.06 nmol/mg ptn) than 2K1C-St (0.53 ± 0.22 nmol/mg ptn) in ventrolateral medulla. Furthermore, the kidneys of 2K1C-EE (11.9 ± 2.3 U/mg ptn) animals presented higher superoxide-dismutase activity than those of 2K1C-St animals (9.1 ± 2.3 U/mg ptn). Conclusion: Environmental enrichment induced an antioxidant effect in the ventrolateral medulla and kidneys that contributes to reducing oxidative damage among hypertensive rats.
DOI: 10.6084/m9.figshare.11313989.v1
title: Language teaching: a comparison of social representations held by first- and fifth-year students of the Translation Studies undergraduate programme at Universidad de Santiago de Chile

description: ABSTRACT This article compares the social representations about the processes of teaching and learning a foreign language held by first- and fifth-year students of the translation studies undergraduate programme at Universidad de Santiago de Chile (USACH). The concept of social representations is taken from social psychology (Jodelet 1988, 2008; Moscovici 1979).A word association task and a semi structured interview were administered to four groups of translator trainees. Results show significant changes in terms of language proficiency, teaching and learning. It is concluded that such changes would promote the adoption of new methodologies for the foreign language course units in this translation programme.
DOI: 10.6084/m9.figshare.11313977
title: Particles in Sikuani

description: Abstract This work analyzes a specific group of morphemes in the Sikuani language (Guahibo) to show that (1) at the form level, the group displays class properties; (2) at the function level, it has semantic and pragmatic correlates that are typically grammatical; and (3) it clearly can be characterized in terms of prototypicity. In this analysis we pursue explicitness and exhaustivity, conducting a first-pass identification of the morpheme classes, inventorying the morphemes in question and providing corresponding examples, conducting a second and more detailed identification of the morpheme classes, and finally summarizing the results. Together, the findings can contribute to the frequently questioned typological notion of a ‘particle.’
DOI: 10.6084/m9.figshare.11313986.v1
title: Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle

description: ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A&gt;G (CAST/DdeI) and AF248054.2:g.6545C&gt;T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels.
DOI: 10.6084/m9.figshare.11313965
title: Synthesis, characterization and antimicrobial activity of Cr (III), Co (II) and Ni (II) complexes with 2-thiazoline-2-tiol derivative ligands against bacteria and yeasts of clinical importance

description: Abstract: Four novel Cr (III), Co (II) and Ni (II) complexes with 2-thiazoline-2-tiol derivative ligands were synthesized and characterized using elemental, physiochemical, and spectroscopy analytical methods. The results showed the formation of ligands coordinated by sulfur atoms of the C = S bond to the L1 ligand and by the sulfur atom of the C-S bond and/or nitrogen of the intra C-ring bond of the L2 ligand. The activity of the complexes against Gram-negative, Gram-positive bacteria and yeasts of clinical importance was evaluated by broth microdilution method. The best result was obtained to L2-Ni compound against E. cloacae (Minimal Inhibitory Concentration (MIC) = 62.5 µg/mL). Additionally, the compounds showed anti-Candida activity (MIC values ranging from 250 to 500 µg/mL), except the L1-Cr that was not active against any tested microorganism.
DOI: 10.6084/m9.figshare.11313968
title: Effects of bagging or the combination of umbrella and bag treatments on anthocyanin accumulation in the berry skin of ‘Kyoho’ (Vitis labruscana) Grape

description: Abstract The object of this study was to investigate the influence of bagging or the combination of umbrella and bags on berry composition, especially anthocyanin accumulation, in ‘Kyoho’ (V. labruscana) berry skins. Five different colors and bag materials commonly used in production were chosen for this study: white, green, yellow, nonwoven and transparent, and the umbrellas were made of plastic material. The composition and content of anthocyanins in the berry skin of ‘Kyoho’ were analyzed by HPLC-MS. The results showed that bagging or a combination of umbrella and bags on anthocyanin concentration in ‘Kyoho’ grapes showed inconsistent effects that depended on the colors and materials of the bags and the use of an umbrella or not. Compared with the control, the inhibition effects of white, green and yellow bags on the soluble solids content and the total and individual anthocyanin concentrations in ‘Kyoho’ grapes reached significant levels; effects of green and yellow bags were the most significant among them. The plastic umbrella used in this study also presented a lower relative light intensity and produced more even-colored clusters. White, green, and yellow bags and combinations of umbrella and bags significantly increased some stable anthocyanin proportions to varying degrees, such as coumaroylated and methylated anthocyanins, compared to the control.
DOI: 10.6084/m9.figshare.11313977.v1
title: Particles in Sikuani

description: Abstract This work analyzes a specific group of morphemes in the Sikuani language (Guahibo) to show that (1) at the form level, the group displays class properties; (2) at the function level, it has semantic and pragmatic correlates that are typically grammatical; and (3) it clearly can be characterized in terms of prototypicity. In this analysis we pursue explicitness and exhaustivity, conducting a first-pass identification of the morpheme classes, inventorying the morphemes in question and providing corresponding examples, conducting a second and more detailed identification of the morpheme classes, and finally summarizing the results. Together, the findings can contribute to the frequently questioned typological notion of a ‘particle.’
DOI: 10.6084/m9.figshare.11313965.v1
title: Synthesis, characterization and antimicrobial activity of Cr (III), Co (II) and Ni (II) complexes with 2-thiazoline-2-tiol derivative ligands against bacteria and yeasts of clinical importance

description: Abstract: Four novel Cr (III), Co (II) and Ni (II) complexes with 2-thiazoline-2-tiol derivative ligands were synthesized and characterized using elemental, physiochemical, and spectroscopy analytical methods. The results showed the formation of ligands coordinated by sulfur atoms of the C = S bond to the L1 ligand and by the sulfur atom of the C-S bond and/or nitrogen of the intra C-ring bond of the L2 ligand. The activity of the complexes against Gram-negative, Gram-positive bacteria and yeasts of clinical importance was evaluated by broth microdilution method. The best result was obtained to L2-Ni compound against E. cloacae (Minimal Inhibitory Concentration (MIC) = 62.5 µg/mL). Additionally, the compounds showed anti-Candida activity (MIC values ranging from 250 to 500 µg/mL), except the L1-Cr that was not active against any tested microorganism.