DOI: 10.5061/dryad.08kprr57x
title: Within-female sperm allocation—Males inseminate at three different female body sites according to female mating history in a squid

description: Theory predicts that males should allocate their costly sperm according to
 future mate availability and focal female conditions, e.g. promiscuity and
 fecundity, to maximise lifetime reproductive success. Some squids show
 insemination site polymorphism within a female—males deposit sperm
 capsules at different female body locations. Whether the choice of
 insemination site is influenced by the sperm-storing status of the female
 remains untested. We studied the squid Loliolus sumatrensis – the females
 possess three insemination sites: the buccal membrane (BM), basal left IV
 arm (ARM) and lateral head behind the left eye (EYE). Analysis of seasonal
 dynamics identified a set priority for the initial use of insemination
 sites as BM then ARM then EYE, whereas the maximum number of stored
 spermatangia was greater as EYE&gt;ARM&gt;BM. The maturity status
 of females was correlated with the pattern, but not number, of
 inseminations. We found multiple paternity at all sites and the same
 genotypes in spermatangia collected from all three sites, suggesting male
 squids allocate their sperm to multiple insemination sites during a single
 mating episode according to the status of female sperm-storage. This study
 uncovered unusual within-female sperm allocation – male mating behaviours
 were influenced by female mating history.
description: Provenance for this README --- * File name: README.MD * Authors: Azad,
 Alam, Nagata, Tomano, Ono, Sugai and Hirohashi * Date created: 2023-10-06
 Dataset Version and Release History --- * Current Version: * Number: 1.0.0
 * Date: 2023-10-06 * Persistent identifier: DOI: 10.5061/dryad.08kprr57x *
 Summary of changes: n/a Dataset Attribution and Usage --- * Dataset Title:
 Data for the article "Within-female sperm allocation—Males inseminate
 at three different female body sites according to female mating history in
 a squid." * Persistent identifier: DOI: 10.5061/dryad.08kprr57x *
 Dataset contributors: Kamrun Naher Azad, Noritaka Hirohashi * Dataset
 citation: <br> Kamrun Naher Azad, Md. Nur E-Alam, Makoto Nagata,
 Satoshi Tomano, Hiroki Ono, Kyoko Sugai, Noritaka Hirohashi (2022),
 Within-female sperm allocation—Males inseminate at three different female
 body sites according to female mating history in a squid. Dryad Digital
 Repository. (doi.10.5061/dryad.08kprr57x). Contact Information --- * Name:
 Noritaka Hirohashi * Affiliations: Graduate School of Natural Science and
 Technology, Shimane University, Japan * ORCID ID:
 https://orcid.org/0000-0001-9264-9517 * Email: hiro@life.shimane-u.ac.jp *
 Address: e-mail preferred Detailed Information of the Dataset --- *
 Description of the dataset: This dataset contains squid individual somatic
 data obtained from 397 males and 520 females on eight different fishing
 days in 2021 and 2022. Methods The squid, Loliolus sumatrensis, were
 obtained from a fisherman at the Shodo Island, Kagawa, Japan during the
 fishery season (July-September) of this species in 2021 and 2022. the
 squid specimens were measured (mostly within one day after fishing) for
 dorsal mantle length (ML), body weight (BW), accessory gland weight (ACC),
 testis weight (TW), relative testis weight (RTW), ovary weight (OW),
 relative ovary weight (ROW), number of insemination sites used (SITE) and
 number of spermatangium attached to the female. To measure somatic weight,
 gonad weight (OW + ACC) was subtracted from total body weight. Methods of
 data collection/generation: see manuscript for more details File details
 --- * This dataset consists of: * The main analysis dataset, included
 directly in DataDryad as an Excel spreadsheet (Adad\_master\_dataset.xlsx)
 which contains the following worksheets: * Somatic indices of adult male
 individuals in L. sumatrensis [sheet name; males] This file contains the
 data of somatic indices; mantle length, body weight, accessory
 reproductive gland weight, testis weight, relative testicular weight
 (TSI). * Somatic indices of adult female individuals in L. sumatrensis
 [sheet name; females(+sperm\_count) ] This file contains the data of
 somatic indices; mantle length, body weight, accessory reproductive gland
 weight, relative ovary weight (OSI), # of insemination sites used by
 males, # of spermatangia attached to each insemination site.BM: buccal
 membrane, ARM: left IV arm, EYE: behind the left eye. * Somatic indices of
 adult female individuals in L. sumatrensis [sheet name;
 females(-sperm\_count) ] This file contains the data of somatic indices;
 mantle length, body weight, accessory reproductive gland weight, relative
 ovary weight (OSI). * Sperm size [sheet name; sperm]: This file contains
 the data of sperm flagellum length. Specimens collected from the male
 reproductive organ (spermatophoric sac) as well as the spermatangia
 attached to female body locations (BM, ARM and EYE). * SSR development
 (sheet name; SSR): This file contains the data of fragment lengths of four
 SSR alleles determined by using genomic DNAs of 42 individuals. ***
 Sharing/access Information --- This dataset has not yet been deposited
 anywhere else. *** END OF THE README