DOI: 10.5061/dryad.nb526
title: Data from: Correlation of shell phenotype and local environment suggests a role for natural selection in the evolution of Placostylus snails

description: The giant edible Placostylus snails of New Caledonia occur across a wide
 range of environmental conditions, from the dry southwest to the wetter
 central and northeastern regions. In large, slow-moving animals such as
 Placostylus, speciation could be assumed to be largely driven by allopatry
 and genetic drift as opposed to natural selection. We examined variation
 in shell morphology using geometric morphometrics and genetic structure
 within two species of Placostylus (P. fibratus, P. porphyrostomus), to
 determine the drivers of diversity in this group. Despite the current
 patchy distribution of snails on New Caledonia, both mtDNA and nuclear SNP
 data sets (&gt;3000 loci) showed weak admixing between populations and
 species. Shell morphology was concordant with the genetic clusters we
 identified and had a strong relationship with local environment. The
 genetic data, in contrast to the morphological data, did not show
 concordance with climatic conditions, suggesting the snails are not
 limited in their ability to adapt to different environments. In sympatry,
 P. fibratus and P. porphyrostomus maintained genetic and morphological
 differences, suggesting a genetic basis of phenotypic variation.
 Convergence of shell shape was observed in two adjacent populations that
 are genetically isolated but experience similar habitat and climatic
 conditions. Conversely, some populations in contrasting environments were
 morphologically distinct although genetically indistinguishable. We infer
 that morphological divergence in the Placostylus snails of New Caledonia
 is mediated by adaptation to the local environment.
description: Set Up Spread
 Sheet-Codes Barcodes etcSpreadsheet describing
 individuals species and collection location. Second sheet describes the
 inline barcodes and illumina index used for demultiplexing. Third sheet
 has Long Lat information for samplesSetUpSpreadSheet.xlsxMorphometric DataMorphometric Dataset. Coordinates and Species/Population/Code classifiersPlacoAllinSlidV2.txtND2 Sequences Placostylus Aligned100 individuals ND2 sequence. Codes match spreadsheetND2Placo.phyND2 assembled GeneAssembly of placostylus mtDNA reads. Covers ND2, CO1, ND4, ND3,COX3ND2assembledGene.fastaMain SNP file STRUCTURE formatDataset of 3764 SNP structure file. Population information included in second column.MainDataSetSTRUCTUREfile.txtNon Neutral SNP file STRUCTURE formatResults from Bayescan non-neutral SNP loci detected. Population ID included in STRUCTURE fileNonNeutralSNPstructure.txtEnvironmental DataEnvironmental Data for populations used on correlation analysisEnvirodatagrandTerreOvAlldatapops.csvLargest SNP dataset statistics results from Stacksparameters set -m 8 –N 2 –M 4 –n 3 -t. A filtered version of this dataset was used in analysis in paper. This dataset required SNPs occur across 2 populations at 90% to be called.Archive 2.zipSTACKS dataset statistics dataset Number 2parameters set -m 8 –N 2 –M 4 –n 3 -t. This dataset required SNP occur across all populations at any coverageArchive.zipStructure file for sympatric populationsSTRUCTURE file for sympatric populationsstructureSYMPATRICpopNOSINGLESp2r5sym.txtSTACKS dataset statistics for sympatric datasetStacks raw statistics for sympatric datasetArchive.zipSTACKS dataset statistics dataset Number 3Raw statistics for SNP dataset number 3 (settings -m 8 –N 2 –M 4 –n 3 -t). This dataset required SNPs to occur in 90% of individuals across 3 populations before being calledArchive.zipIndex_E part 1 Raw RAD readsRaw RAD reads. Barcodes and Codes can be found in SpreadsheetArchive.zipIndex e part 2 Raw RAD readsSee spread sheet for individual details. De multiplexed Index E part 2.Archive 2.zipIndex_F part 1 Raw RAD readsIndex_F part 1 raw RAD reads. Codes correlate to spreadsheet.Archive.zipIndex_F part 2 Raw RAD readsIndex_F part 2 Raw RAD reads. Codes and barcodes located in spreadsheetArchive 2.zipIndex_G part 1 Raw RAD readsIndex_G part 1 Raw RAD reads. Codes in spreadsheet.Archive.zipIndex_G part 2 Raw RAD readsIndex_G part 2 Raw RAD reads. Codes in spreadsheetArchive 2.zipIndex_H part 1 Raw RAD readsIndex_H part 1 Raw RAD reads. Codes in SpreadsheetArchive.zipIndex_H part 2 Raw RAD readsIndex_H part 2 Raw RAD reads. Codes in SpreadsheetArchive 2.zipIndex_I part 1 Raw RAD readsIndex_I part 1 Raw RAD reads. Codes in spreadsheetArchive.zipIndex_I part 2 Raw RAD readsIndex_I part 2 Raw RAD reads. Codes in spreadsheetArchive 2.zipForward Read Genomic DNAForward Read used to assembled partial mtDNA genome of Placostylus. Sample from Mépouiri. Sequenced HiSeq120223_I238_FCC0HH4ACXX_L5_SZAXPI005962-142_1.fq.gzReverse Read Genomic DNAReverse Read. Genomic DNA placostylus120223_I238_FCC0HH4ACXX_L5_SZAXPI005962-142_2.trimmed.paired.fq.gzRaxMLTreeBSTRAPplacoND2Raw tree file from RaxML of ND2 genePlacostylus Morphometric Data Better explainedSee comments in file for explanation. Includes the same data as the other morphological data file but is better explained. Includes Raw and Procrustes Distance co-ordinates.PlacostylusMorphometricData.xlsx