DOI: 10.5061/dryad.zkh1893mz
title: GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration

description: The saltatory migration of neurons is essential for brain formation.
 Whether mechanical stimuli regulate this process is unknown. Here we show
 that the primary cilium acts as a mechanical sensor through GPR161. Using
 an ex vivo neuronal migration model and microfluidic
 assays, we demonstrate that fluid shear stress induces
 migration via the mechanoreceptor GPR161 at the primary
 cilium, with its mechanosensitive Helix 8 being essential. We demonstrate
 that GPR161 activates a recently discovered cAMP/PKA signaling pathway
 leading to the phosphorylation of NDE1, a dynein complex regulator, and
 microtubule organization to regulate migration. These findings unveil a
 dynamic primary cilium-based pathway sensing mechanical stimulus to drive
 cyclic saltatory neuronal migration during brain development.
description: # GPR161 mechanosensitivity at the primary cilium drives neuronal
 saltatory migration†: Open Source Data
 [https://doi.org/10.5061/dryad.zkh1893mz](https://doi.org/10.5061/dryad.zkh1893mz) Other publicly accessible locations of the data: [https://dropsu.sorbonne-universite.fr/s/aBwfsN7F2sFt9Q8](https://dropsu.sorbonne-universite.fr/s/aBwfsN7F2sFt9Q8) --- We have submitted our raw data for the paper "GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration". This includes the raw data: \- Fig. 1D \- Fig. 1F \- Fig. 1G \- Fig. 1HIJ \- Fig. 2C \- Fig. 2D \- Fig. 2E \- Fig. 2F \- Fig. 3C \- Fig. 3D \- Fig. 3G \- Fig. 4D \- Fig. 4EFG \- Fig. S2D \- Fig. S3B \- Fig. S4 ## Description of the data and file structure Each file contains a simple Excel file of the raw data of quantified parameters. ### Title: Fig. 1D Excel table presenting the number of GFP+ RMS neuroblasts presenting a GPR161+/Arl13b+ or GPR161-/Arl13b+ immunoreactive PC in miRNeg electroporated neuroblasts (N=3, n=51). ### Title: Fig. 1F Excel table presenting the number of neuroblasts with internalized or externalized PC, in neuroblasts in a pausing morphology or in a migrating morphology based on the nucleus-to-cilium (N-C) distance, in miRNeg condition (N=3, n=53). ### Title: Fig. 1G Excel table presenting the number of Arl13b+/GPR161+ or Arl13b+/ GPR161-immunoreactive PCs in their internalized and externalized states, in miRNeg condition (N=3, n=51). ### Title: Fig. 1HIJ Excel table of the analysis of the rhythm of migration for each neuron in different conditions (miRNeg or miRGPR161CDS or miRGPR161UTR+GPR161H8del, or miRGPR161UTR+GPR161wt). In this table are presented the data for the parameters: migration speed (micrometers per hour), nucleokinesis frequency (per hour), and pausing time (percentage). ### Title: Fig. 2C Excel table presenting the number of neuroblasts with internalized or externalized PC, in neuroblasts in a pausing morphology or a migrating morphology based on the nucleus-to-cilium (N-C) distance, in the control condition (N=5, n=44). ### Title: Fig. 2D Excel table presenting the number of 2D-cultured neuroblasts displaying an Arl13b+ and GPR161 immunoreactive (GPR161+) or not (GPR161-) PC, in control condition (N=3, n=26). ### Title: Fig. 2E Excel table presenting the number of Arl13b+ and GPR161 immunoreactive (GPR161+) or not (GPR161-) PC in its internalized and externalized states, in control condition (N=3, n=24). ### Title: Fig. 2F Excel table presenting the number of neuroblasts performing at least one nucleokinesis (NK) during the entire movie experiment (2.5 hours) in no-flow (N=3, n=71) condition, flow condition (N=3, n=75), LvmiRNeg+Flow (N=3, n=77) or LvmiRGPR161+Flow (N=3, n=65) conditions, with a flow corresponding to a shear stress of 0.13Pa. ### Title: Fig. 3C Excel table presenting the number of straight vs. bent microtubular cages in miRNeg (N=3, n=46) and miRGPR161CDS (N=5, n=61) conditions. ### Title: Fig. 3D Excel table presenting the number of rear-fasciculated vs. rear-disorganized microtubular cages in miRNeg (N=3, n=46) and miRGPR161CDS (N=5, n=61) conditions. ### Title: Fig. 3G Excel table presenting the number of migrating neuroblasts with or without the presence of a cAMP hotspot in control (N=9, n=52) and miRGPR161CDS conditions (N=6, n=34). ### Title: Fig. 4D Excel table presenting the ratio of the mean intensities of NDE1 at the centrosome in miRNeg (N=3) and miRGPR161CDS (N=4) conditions, during pausing time (miRNeg: n=15, miRGPR161CDS: n=14) and CK phase (miRNeg: n=21, miRGPR161CDS: n=23). ### Title: Fig. 4EFG Excel table of the analysis of the rhythm of migration for each neuron in different conditions(miRNeg or miRGPR161CDS or Nde1PMutant, or miRGPR161CDS+Nde1pmimic). In this table are presented the data for the parameters: migration speed (micrometers per hour), nucleokinesis frequency (per hour), and pausing time (percentage). ### Title: Fig. S2D Excel table presenting the number of GFP+ RMS neuroblasts with a GPR161+/Arl13b+ or GPR161-/Arl13b+ immunoreactive PC in different conditions: control miRNeg (N=3 n=53), miRGPR161CDS (N=3 n=60) or miRGPR161UTR (N=3 n=38) ### Title: Fig. S3B Excel table presenting the number of cells migrating in the direction of the flow, or in the opposite direction, in the different conditions: no flow, flow, LvMiRNeg+flow, LvMiRGPR161+flow. ### Title: Fig. S4 Excel table of the global analysis of the rhythm of migration for each neuron in all conditions (miRNeg or miRGPR161CDS or miRGPR161UTR+GPR161H8del or miRGPR161UTR+GPR161wt or Nde1PMutant or miRGPR161CDS+Nde1pmimic). In this table are presented the data for the following parameters: migration speed (micrometers per hour), nucleokinesis frequency (per hour), and pausing time (percentage). miRNeg (N=6, n=227), miRGPR161CDS (N=7, n=203), miRGPR161UTR+GPR161wt (N=3, n=139), miRGPR161UTR+GPR161H8del (N=3, n=114), Nde1PMutant (N=4, n=128) or miRGPR161CDS+Nde1pmimic (N=3, n=88). ## Sharing/Access information NA ## Code/Software NA