cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.syqfhx
title: Occurrence Download

description: A dataset listing the 1125642 species recorded in GBIF matching the query:
{
 "TaxonKey" : [
 "is Plantae"
 ]
}

 The dataset's 1125642 records were derived from 17588 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011981-250415084134356/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.puea8v
title: Occurrence Download

description: A dataset containing 157 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-79.21902 -4.21131,-78.87651 -4.21131,-78.87651 -3.79469,-79.21902 -3.79469,-79.21902 -4.21131))",
 "TaxonKey is Tremarctos ornatus (F.G.Cuvier, 1825)"
 ]
}

 The dataset includes 157 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002416-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rspkhb
title: Occurrence Download

description: A dataset containing 597 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lampanyctus intricarius Tåning, 1928",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 597 records from 35 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001982-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.j9g5c7
title: Occurrence Download

description: A dataset containing 251 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Brazil",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Euterpe oleracea Mart."
 ]
}

 The dataset includes 251 records from 43 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000334-250424135625364/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.v2weqh
title: Occurrence Download

description: A dataset containing 308 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Picoides arcticus (Swainson, 1832)",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is Specimen",
 "Year is greater than or equal to 1810",
 "Year is less than or equal to 2019",
 "Month is greater than or equal to April",
 "Month is less than or equal to September",
 "EventDate is not null",
 "Country is United States of America",
 "Geometry POLYGON((-63 22, -63 51, -126 51, -126 22, -63 22))"
 ]
}

 The dataset includes 308 records from 33 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010899-250515123054153/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15550215
title: CODEBOOK - Completing Exploratory Observations in Irish Women's Health Services: A Tool to Scope Opportunities for Responsible Design

description: This supplementary document outlines the full dataset of codes resulting from the analysis of exploratory observations. The tables below outline the codes resulting from all three rounds of coding (Stage 3, Phase 2: Coding Rounds 1-3).
DOI: 10.5281/zenodo.15550216
title: CODEBOOK - Completing Exploratory Observations in Irish Women's Health Services: A Tool to Scope Opportunities for Responsible Design

description: This supplementary document outlines the full dataset of codes resulting from the analysis of exploratory observations. The tables below outline the codes resulting from all three rounds of coding (Stage 3, Phase 2: Coding Rounds 1-3).
DOI: 10.15468/dl.3dvupb
title: Occurrence Download

description: A dataset containing 12 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eleusine indica (L.) Gaertn.",
 "Country is Rwanda"
 ]
}

 The dataset includes 12 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008103-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7qncym
title: Occurrence Download

description: A dataset listing the 17 species recorded in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((44.89125 41.55222,44.896 41.50186,44.91982 41.45649,44.96689 41.43324,45.02417 41.43097,45.07294 41.43947,45.1047 41.47237,45.12512 41.50413,45.09371 41.55619,45.07897 41.6095,44.98879 41.61744,44.92584 41.59986,44.89125 41.55222))",
 "OccurrenceStatus is Present",
 "TaxonKey is Mammalia",
 "Year 2010-2025"
 ]
}

 The dataset's 17 records were derived from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011055-250415084134356/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.35097/1546
title: Reconstructed ptychographic X-ray computed tomography data sets of the dried gel Ni/Al$_{2}$O$_{3}$-dr, Al$_{2}$O$_{3}$-dr samples and the calcined samples Ni/Al$_{2}$O$_{3}$-calc, Al$_{2}$O$_{3}$-calc

description: Reconstructed ptychographic X-ray computed tomography data sets acquired at Swiss Light Source, Beamline cSAXS (proposal MG24079-1) of the dried gels Al2O3-dr and NiAl2O3-dr and the calcined samples Al2O3-calc and NiAl2O3-calc of the identical particles. Detailed information regarding sample preparation and experimental conditions are available in the respective publication and electronic supplementary information.
description: The data contains the reconstructed tomogram slices in a ".tiff" format as delta contrast tomogram. The correction of the delta tomogram and calculation of the electron density tomogram has to be done as described in the ".txt" files. Information about required offset correction of the electron density tomograms is provided in the respective publication. Reconstructed X-ray ptychography projections were tomographically reconstructed using the Matlab script of the cSAXS group of the Swiss Light Source, as published in DOI:10.5281/zenodo.3539550 and DOI:10.1364/OE.27.036637. Ptychographic projections were obtained as described in the respective publication and electronic supplementary information.
DOI: 10.15468/dl.dssehr
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Monitoreos de Fauna y Flora en áreas biosensoras y de rehabilitación de la Mina Calenturitas, 2020-2022",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 5 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029384-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3arbcv
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is CNMA/Colección de Mamíferos de la Estación de Biología Chamela",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 5 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029385-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.haq4se
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "DatasetKey is Monitoreo de fauna amenazada y cambios poblacionales asociados a las fichas del PMA del Gasoducto Paiva-Caracolí",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029386-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.n2tyck
title: Occurrence Download

description: A dataset containing 5588 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is University of Florida Herpetology"
 ]
}

 The dataset includes 5588 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029387-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2e84g9
title: Occurrence Download

description: A dataset containing 17797 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "TaxonKey is Cryptandra Sm."
 ]
}

 The dataset includes 17797 records from 80 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029388-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.bw2bwu
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Caracterización de fauna para el establecimiento de nuevas Reservas Naturales de la Sociedad Civil en Arauca",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 5 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029389-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.tynbzn
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Registros de fauna obtenidos por medio de fototrampeo en la zona de influencia del proyecto de exploración de hidrocarburos Pozo Cascabel, Hato Corozal",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 5 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029390-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.s6j5x9
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Mamíferos medianos y grandes de la Central Hidroeléctrica San Carlos (Antioquia), en el primer semestre del año 2021",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029391-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qg4w9t
title: Occurrence Download

description: A dataset containing 6330 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Costa Rica",
 "Geometry POLYGON((-180 -90,180 -90,180 90,-180 90,-180 -90))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Alouatta palliata (Gray, 1849)"
 ]
}

 The dataset includes 6330 records from 20 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029392-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7qnary
title: Occurrence Download

description: A dataset containing 10885 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((-79.28075022581828 -3.236679362852491, -79.28053437573688 -3.236633780410489, -79.28049841851814 -3.236615633256541, -79.27610700674241 -3.231824433361089, -79.27560420530007 -3.231217699666228, -79.27550314179729 -3.231078303619705, -79.27531700821145 -3.230801235218154, -79.27527220715812 -3.230692643700107, -79.27452076206424 -3.228150300702753, -79.27451185681 -3.228096027987207, -79.27411206545155 -3.225093096720323, -79.27410453257292 -3.224224963513794, -79.27520470049429 -3.222707625392411, -79.29774968019765 -3.215405224505481, -79.30142072626265 -3.219441703355947, -79.30154566024325 -3.221110522415302, -79.30141140304598 -3.225063619086928, -79.30130834818698 -3.22560253551913, -79.29011832807603 -3.235338827588457, -79.2888213548745 -3.236177628403193, -79.28075022581828 -3.236679362852491))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Animalia"
 ]
}

 The dataset includes 10885 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029394-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8nsfyz
title: Occurrence Download

description: A dataset containing 40545 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "TaxonKey is Cryptantha Lehm. ex Fisch. & C.A.Mey."
 ]
}

 The dataset includes 40545 records from 211 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029393-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.cezjyy
title: Occurrence Download

description: A dataset containing 388 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Micromelum falcatum (Lour.) Yu.Tanaka)",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Living Specimen, Machine Observation, Literature Occurrence)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 388 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029396-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.tyj2yw
title: Occurrence Download

description: A dataset containing 2033045 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 }
 ]
 },
 "TaxonKey is Spizelloides arborea (A.Wilson, 1810)",
 "Year is greater than or equal to 1950",
 {
 "or" : [
 "GadmGid is USA",
 "GadmGid is CAN",
 "GadmGid is MEX"
 ]
 }
 ]
}

 The dataset includes 2033045 records from 68 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029368-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z95rdh
title: Occurrence Download

description: A dataset containing 59368 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ginkgo biloba L."
 ]
}

 The dataset includes 59368 records from 677 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029381-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.g9mtqd
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Monitoreo de fauna silvestre en la zona de reubicación y margen derecha del Embalse El Quimbo",
 "OccurrenceStatus is Present",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029398-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ved4xj
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Monitoreo de especies focales de fauna y flora en áreas priorizadas en el Valle del Cauca",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029397-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nscq2w
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Estudio de Biodiversidad Mina Calenturitas, Temporada de Lluvias",
 "TaxonKey is Puma yagouaroundi (É.Geoffroy Saint-Hilaire, 1803)"
 ]
}

 The dataset includes 4 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029399-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2g6jwq
title: Occurrence Download

description: A dataset containing 19 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((-79.28075022581828 -3.236679362852491, -79.28053437573688 -3.236633780410489, -79.28049841851814 -3.236615633256541, -79.27610700674241 -3.231824433361089, -79.27560420530007 -3.231217699666228, -79.27550314179729 -3.231078303619705, -79.27531700821145 -3.230801235218154, -79.27527220715812 -3.230692643700107, -79.27452076206424 -3.228150300702753, -79.27451185681 -3.228096027987207, -79.27411206545155 -3.225093096720323, -79.27410453257292 -3.224224963513794, -79.27520470049429 -3.222707625392411, -79.29774968019765 -3.215405224505481, -79.30142072626265 -3.219441703355947, -79.30154566024325 -3.221110522415302, -79.30141140304598 -3.225063619086928, -79.30130834818698 -3.22560253551913, -79.29011832807603 -3.235338827588457, -79.2888213548745 -3.236177628403193, -79.28075022581828 -3.236679362852491))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Plantae"
 ]
}

 The dataset includes 19 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0029401-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29061095
title: 初始data.xlsx

description: <br>PCR：Total RNA was extracted from rat brain tissue using Trizol reagent (Invitrogen, USA), while miRNA was isolated using a miRNA purification kit (CW0627S, CWBIO, China). RNA concentration and purity were measured using an NP80 UV spectrophotometer (NanoPhotometer, Germany), and samples with OD260/OD280 ratios between 1.8-2.0 were considered acceptable. miRNA was reverse transcribed into cDNA using miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (MR101-02, Vazyme, China). qPCR reactions were performed using miRNA Universal SYBR qPCR Master Mix (MQ101-02, Vazyme, China) on a CFX Connect™ fluorescence PCR system (Bio-Rad, USA). The reaction protocol was as follows: pre-denaturation at 95°C for 10 min; denaturation at 95°C for 10 s; annealing at 58°C for 30 s; extension at 72°C for 30 s; for 40 cycles. Each sample was analyzed in triplicate using U6 as an internal reference. Relative gene expression was calculated using the 2^-ΔΔCT method. Primer sequences are listed in the Table1 below. ELISA：ELISA was used to determine the levels of IL-1β and IL-18. Rat serum was collected and centrifuged at 1000g for 20 min. The assay was performed according to the manufacturer's instructions (ExCell Biology, Shanghai, China). The specific procedure was as follows The ELISA kit was equilibrated at room temperature. Standards were prepared according to the manufacturer's instructions. Blank, standard and sample wells were prepared. No samples or enzyme-labelled reagents were added to the blank wells. 50 μL of sample or standard was added to the remaining wells, followed by 100 μL of enzyme-labelled reagent. The plate was incubated for 1 hour at 37°C. After five washes, chromogenic substrate was added and incubated at 37°C for 15 minutes. The reaction was stopped by adding stop solution and the absorbance of each well was measured at a wavelength of 450 nm.WB： 收集大鼠海马组织，并在含有蛋白酶和磷酸酶抑制剂的 RIPA 裂解缓冲液中匀浆，使用组织匀浆器提取总蛋白。将匀浆在 4°C 下以 12,000 rpm 离心 10 分钟，并收集上清液。使用 BCA 蛋白检测试剂盒进行蛋白定量。蛋白质变性后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 （SDS-PAGE） 分离样品 1.5 h。将蛋白质以 300 mA 的恒定电流转移到 PVDF 膜 （Millipore） 上，持续不同的持续时间：NRG1 为 1 小时，ErbB4、TLR4 和 NLRP3 为 2 小时，ASC 为 0.5 小时。用脱脂牛奶封闭 PVDF 膜，然后在 4°C 下与一抗孵育过夜。 第二天，将膜与二抗在室温下孵育 2 小时。用化学发光试剂润湿膜，并使用高灵敏度化学发光成像系统进行可视化。使用 Image J 软件分析条带强度，β-肌动蛋白用作内部参考来计算靶蛋白的相对表达。
DOI: 10.6084/m9.figshare.29061095.v1
title: 初始data.xlsx

description: <br>PCR：Total RNA was extracted from rat brain tissue using Trizol reagent (Invitrogen, USA), while miRNA was isolated using a miRNA purification kit (CW0627S, CWBIO, China). RNA concentration and purity were measured using an NP80 UV spectrophotometer (NanoPhotometer, Germany), and samples with OD260/OD280 ratios between 1.8-2.0 were considered acceptable. miRNA was reverse transcribed into cDNA using miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (MR101-02, Vazyme, China). qPCR reactions were performed using miRNA Universal SYBR qPCR Master Mix (MQ101-02, Vazyme, China) on a CFX Connect™ fluorescence PCR system (Bio-Rad, USA). The reaction protocol was as follows: pre-denaturation at 95°C for 10 min; denaturation at 95°C for 10 s; annealing at 58°C for 30 s; extension at 72°C for 30 s; for 40 cycles. Each sample was analyzed in triplicate using U6 as an internal reference. Relative gene expression was calculated using the 2^-ΔΔCT method. Primer sequences are listed in the Table1 below. ELISA：ELISA was used to determine the levels of IL-1β and IL-18. Rat serum was collected and centrifuged at 1000g for 20 min. The assay was performed according to the manufacturer's instructions (ExCell Biology, Shanghai, China). The specific procedure was as follows The ELISA kit was equilibrated at room temperature. Standards were prepared according to the manufacturer's instructions. Blank, standard and sample wells were prepared. No samples or enzyme-labelled reagents were added to the blank wells. 50 μL of sample or standard was added to the remaining wells, followed by 100 μL of enzyme-labelled reagent. The plate was incubated for 1 hour at 37°C. After five washes, chromogenic substrate was added and incubated at 37°C for 15 minutes. The reaction was stopped by adding stop solution and the absorbance of each well was measured at a wavelength of 450 nm.WB： 收集大鼠海马组织，并在含有蛋白酶和磷酸酶抑制剂的 RIPA 裂解缓冲液中匀浆，使用组织匀浆器提取总蛋白。将匀浆在 4°C 下以 12,000 rpm 离心 10 分钟，并收集上清液。使用 BCA 蛋白检测试剂盒进行蛋白定量。蛋白质变性后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 （SDS-PAGE） 分离样品 1.5 h。将蛋白质以 300 mA 的恒定电流转移到 PVDF 膜 （Millipore） 上，持续不同的持续时间：NRG1 为 1 小时，ErbB4、TLR4 和 NLRP3 为 2 小时，ASC 为 0.5 小时。用脱脂牛奶封闭 PVDF 膜，然后在 4°C 下与一抗孵育过夜。 第二天，将膜与二抗在室温下孵育 2 小时。用化学发光试剂润湿膜，并使用高灵敏度化学发光成像系统进行可视化。使用 Image J 软件分析条带强度，β-肌动蛋白用作内部参考来计算靶蛋白的相对表达。