DOI: 10.5061/dryad.t9j07
title: Data from: Thermal stress and predation risk trigger distinct transcriptomic responses in the intertidal snail Nucella lapillus

description: Thermal stress and predation risk have profound effects on rocky shore
 organisms, triggering changes in their feeding behaviour, morphology and
 metabolism. Studies of thermal stress have shown that underpinning such
 changes in several intertidal species are specific shifts in gene and
 protein expression (e.g. upregulation of heat-shock proteins). But
 relatively few studies have examined genetic responses to predation risk.
 Here, we use next-generation RNA sequencing (RNA-seq) to examine the
 transcriptomic (mRNA) response of the snail Nucella lapillus to thermal
 stress and predation risk. We found that like other intertidal species, N.
 lapillus displays a pronounced genetic response to thermal stress by
 upregulating many heat-shock proteins and other molecular chaperones. In
 contrast, the presence of a crab predator (Carcinus maenas) triggered few
 significant changes in gene expression in our experiment, and this
 response showed no significant overlap with the snail's response to
 thermal stress. These different gene expression profiles suggest that
 thermal stress and predation risk could pose distinct and potentially
 additive challenges for N. lapillus and that genetic responses to biotic
 stresses such as predation risk might be more complex and less uniform
 across species than genetic responses to abiotic stresses such as thermal
 stress.
description: Raw Read Count
 Expression Data for ND Chu et al 2014Total raw read count
 expression data from Nucella lapillus from the accompanying publication.
 This file was created by eXpress (http://bio.math.berkeley.edu/eXpress/)
 for analysis in DEseq2. The sample names are as in the publication. Each
 row represents a transcript, as assembled in Trinity (see publication for
 transcriptome assembly accession; http://trinityrnaseq.sourceforge.net/).
 Experimental snails were collected from near Nahant, Massachusetts,
 USA.NChuetal2014ReadCountTable.txtExpress Results File AC_006Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample AC_006, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_006results.xprsExpress Parameters File AC_006Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample AC_006, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_006params.xprsExpress Results File AC_008Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample AC_006, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_008results.xprsExpress Parameters File AC_008Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample AC_008, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_008params.xprsExpress Results File AC_43Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample AC_43, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_43results.xprsExpress Parameters File AC_43Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample AC_43, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_43params.xprsExpress Results File AC_42Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample AC_42, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_42results.xprsExpress Parameters File AC_42Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample AC_42, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.AC_42params.xprsExpress Results File ANC_003Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample ANC_003, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.ANC_003results.xprsExpress Parameters File ANC_003Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample ANC_003, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.ANC_003params.xprsExpress Results File ANC_34Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample ANC_34, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.ANC_34results.xprsExpress Parameters File ANC_34Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample ANC_34, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.ANC_34params.xprsExpress Results File ANC_41Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample ANC_41, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.ANC_41results.xprsExpress Parameters File ANC_41Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample ANC_41, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.ANC_41params.xprsExpress Results File HNC_31Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample HNC_31, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.HNC_31results.xprsExpress Parameters File HNC_31Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample HNC_31, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.HNC_31params.xprsExpress Results File HNC_37Raw eXpress (http://bio.math.berkeley.edu/eXpress/) results file for sample HNC_37, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.HNC_37results.xprsExpress Parameters File HNC_37Raw eXpress (http://bio.math.berkeley.edu/eXpress/) parameters file for sample HNC_37, named as in Chu et al. 2014. Gene expression data is from Nucella lapillus collected near Nahant, Massachusetts, USA.HNC_37params.xprs