DOI: 10.5061/dryad.xsj3tx9v1
title: The pollination ecology and mouthpart morphology of a pollen-feeding fly <em>Incurviseta</em> cf. <em>maculifrons</em> (Diptera: Lauxaniidae) in the Australian Alpine

description: This dataset was generated to investigate the pollination ecology and
 pollen diet of a pollinivorous Lauxaniid fly: Incurviseta cf.
 maculifrons. The dataset includes data used to characterize the
 visitation, pollen transport, and pollen diet of I. cf. maculifrons.
 Variables include body pollen counts, gut pollen counts, and raw
 visitation data. Specimen metadata provide details on sampling locations,
 collection dates, and floral hosts. Supplementary R code is included to
 demonstrate the generation of figures and tables included in the
 manuscript.
description: # Data and analyses for the manuscript: The pollination ecology and
 mouthpart morphology of a pollen-feeding fly *Incurviseta* cf.
 *maculifrons* (Diptera: Lauxaniidae) in the Australian Alpine --- ##
 Overview Most of the analyses were conducted in **R** and are presented
 here as **R Markdown (.Rmd)** files. These files can be used to reproduce
 the figures and table found in the manuscript. For **Figure 1**, the
 pollen diameters were calculated by averaging the diameter of microscope
 images of pollen grains using imageJ or equivalent image-analysis software
 with a microscope camera. Reference images of pollen types with scale bars
 can be found in the `Pollen_Images.zip` folder, with pollen types
 separated between subdirectories. The .Rmd code is self-explanatory in
 most cases (i.e. pairwise.wilcox.test() is a pairwise wilcox test), but
 some comments are provided where necessary for clarity. Please note that
 text and formatting changes were applied to the generated figures after
 the data analysis step. Therefore, the .Rmd files contain out-of-date
 species names, for example H. scorpioides (meaning Helichrysum
 scorpioides) is Coronidium scorpioides in the final manuscript. --- ##
 File Descriptions ### `Pollen_Images.zip` Reference images of pollen types
 with scale bars, with pollen types separated between subdirectories. ---
 ### `Incurviseta_floral_interactions.csv` This file includes all
 visitation data used in **`Visitation_data_analysis.rmd`**. **Columns:** *
 **Site** – subalpine or alpine * **Date** – date of observation * **Time**
 – time of day that observations occurred * **Plant Species** – plant
 species *I. cf. maculifrons* was observed visiting * **Observed Visits** –
 number of individuals observed visiting --- ###
 `Incurviseta_gut_and_body_pollen.csv` This file includes all body and gut
 pollen count data used in **`Analysing_gut_pollen.rmd`** and
 **`Visitation_data_analysis.rmd`**. **Columns:** * **ID** – unique
 identifier for each individual * **Location** – where pollen was removed
 from (gut or body) * **Sex** – usually unknown unless eggs/larvae were
 present in gut samples, in which case sex=female * **Site** – subalpine or
 alpine * **Date** – date the individual was collected * **Flower** –
 flower individual was collected from * **PollenID** – initial pollen
 identification * **PollenID2** – final pollen ID used for data analysis *
 **Count** – number of pollen grains (gut pollen counts have been
 extrapolated by multiplying raw counts by four, as per the manuscript
 methodology) --- ### `Analysing_gut_pollen.Rmd` Reproduces the analyses
 for gut pollen, including figure creation and statistical tests presented
 in the manuscript. --- ### `Comparing_gut_pollen_to_body_pollen.Rmd`
 Reproduces statistical analyses comparing gut and body pollen data.\ These
 analyses follow the approach outlined in: &gt; Lysenkov, S. N. &
 Galinskaya, T. V. (2017). *Comparison of the pollen content on the body
 and in the gut of hoverflies (Diptera: Syrphidae).* **Entomological
 Review**, 97, 10–16. --- ### `Visitation_data_analysis.Rmd` Reproduces the
 visitation data analysis and generation of **Table 1** in the manuscript,
 which describes the visitation of *Incurviseta cf. maculifrons*.