DOI: 10.5061/dryad.8cz8w9h1x
title: Data from: Amoeboid cells undergo durotaxis with soft end polarized NMIIA

description: Cell migration towards stiff substrates has been coined as durotaxis and
 implicated in development, wound healing, and cancer, where complex
 interplays between immune and non-immune cells are present. Compared to
 the emerging mechanisms underlying the strongly adhesive mesenchymal
 durotaxis, little is known about whether immune cells - migrating in
 amoeboid mode - could follow mechanical cues. Here we develop an
 imaging-based confined migration device with stiffness gradient. By
 tracking live cell trajectory and analyzing the directionality of T cells
 and neutrophils, we observe that amoeboid cells can durotax. We further
 delineate the underlying mechanism to involve non-muscle myosin IIA
 (NMIIA) polarization towards the soft-matrix-side but may not require
 differential actin flow up- or down-stiffness gradient. Using the protista
 Dictyostelium, we demonstrate the evolutionary conservation of amoeboid
 durotaxis. Finally, these experimental phenomena are theoretically
 captured by an active gel model capable of mechanosensing. Collectively,
 these results may shed new lights on immune surveillance and recently
 identified confined migration of cancer cells, within the mechanically
 inhomogeneous tumor microenvironment or the inflamed fibrotic tissues.
description: # Data from: Amoeboid cells undergo durotaxis with soft end polarized
 NMIIA
 [https://doi.org/10.5061/dryad.8cz8w9h1x](https://doi.org/10.5061/dryad.8cz8w9h1x) ## Description of the data and file structure This dataset contains all imaging data, plot data, and code for our paper entitled “Amoeboid cells undergo durotaxis with soft end polarized NMIIA” which is published on* eLife*. The imaging data are source data for Figure 1B, 1F, IJ, Figure 1-figure supplement 1B-D, Figure 1-figure supplement 3A, Figure 2-figure supplement 1, Figure 3B, Figure 3F, Figure 3-figure supplement 1D-E, Figure 3-figure supplement 2A, Figure 3-figure supplement 2C, Figure 4,A and Figure 5C-D. The plot data are source data for Figure 1E, 1I, IM, Figure 2A-2H, Figure 3E, 3G-H, Figure 3-figure supplement 1A, Figure 3-figure supplement 2B, Figure 3-figure supplement 2D, Figure 4D-F and Figure 6G-I. The western data are source data for Figure 3A and Figure 3-figure supplement 1B. The code data are for our active gel modeling. ### Files and variables #### File: imaging\_data.zip **Description:** The imaging data are source data for Figure 1B, 1F, IJ, Figure 1-figure supplement 1B-D, Figure 1-figure supplement 3A, Figure 2-figure supplement 1, Figure 3B, Figure 3F, Figure 3-figure supplement 1D-E, Figure 3-figure supplement 2A, Figure 3-figure supplement 2C, Figure 4A, and Figure 5C-D. #### File: Plot\_data.xlsx **Description:** The plot data are source data for Figure 1E, 1I, IM, Figure 2A-2H, Figure 3E, 3G-H, Figure 3-figure supplement 1A, Figure 3-figure supplement 2B, Figure 3-figure supplement 2D, Figure 4D-F and Figure 6G-I. In the plot data, the unit of velocity is μm/sec and the unit of actin flow speed is nm/min. The yFMI and persistence have no unit. #### File: western\_data.zip **Description:** The western data are source data for Figure 3A and Figure 3-figure supplement 1B. ## Code/software The code data are for our active gel modeling. ## Access information Other publicly accessible locations of the data: * [https://doi.org/10.7554/eLife.96821.3](https://doi.org/10.7554/eLife.96821.3)