cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/jq6-c75-870
title: Escallonia alpina Poepp. ex DC.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/5w0-4k2-lmt
title: Aegilops ventricosa Tausch

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/hyk-tap-mjv
title: Achnatherum calamagrostis (L.) P.Beauv.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/5fx-5ah-jwj
title: Gladiolus palustris Gaudin

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/ds2-jp6-q6b
title: Wedelia calycina Spreng.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/kyk-1yy-1rk
title: Pentacalia involuta (Klatt) Cuatrec.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/6bl-ef2-rvw
title: Gladiolus palustris Gaudin

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/vt8-q5q-epn
title: Sphaeranthus suaveolens (Forssk.) DC.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/bgf-nve-x7f
title: Mouriri grandiflora subsp. grandiflora

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/wp5-7tk-14c
title: Mouriri angustifolia Spruce ex Triana

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.15468/dl.hnwtus
title: Occurrence Download

description: A dataset containing 4543 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Occurrence evidence, Observation)",
 "TaxonKey is one of (Vanellus vanellus)",
 "Country is one of (Bermuda, Canada, Greenland, Saint Pierre and Miquelon, United States of America)"
 ]
}

 The dataset includes 4543 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0070042-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/a0b-029-ctf
title: Mouriri myrtifolia Spruce ex Triana

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/mgf-tb6-qef
title: Bellucia egensis (DC.) Penneys, Michelang., Judd & Almeda

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/v34-5qm-68y
title: Hieracium racemosum subsp. leiopsis Murr & Zahn

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/wbs-173-gw6
title: Miconia holosericea (L.) DC.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/dhr-zdk-0gr
title: Sauvagesia linearifolia A. St.-Hil.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/pym-tmz-39z
title: Mouriri subumbellata Triana

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/1e7-836-rhn
title: Pistorinia breviflora subsp. intermedia (Boiss. & Reut.) H. Jacobsen ex Greuter & Burdet

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/m6l-ezx-13e
title: Kohautia caespitosa subsp. caespitosa

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3886/icpsr06313
title: Schools and Staffing Survey, 1990-1991: [United States]
title: Archival Version

description: This data collection consists of four integrated surveys of 
 public and private schools, administrators, and teachers in the United 
 States. Part 1, the Teacher Demand and Shortage Survey, presents 
 information from schools and local education agencies on student 
 enrollment, number of teachers, position vacancies, new hires, teacher 
 salaries and incentives, and hiring and retirement policies. Part 3, 
 the School Administrator Survey, includes information about training, 
 experience, professional background, and job activities of school 
 principals and headmasters. Questions in Part 5, the Teacher Survey, 
 cover teacher education and training, teaching assignment, teaching 
 experience, certification, teaching workload, perceptions and attitudes 
 about teaching, job mobility, and workplace conditions. Part 7, the 
 School Survey, provides data from elementary and secondary schools on 
 student characteristics, staffing patterns, student-teacher ratios, 
 enrollment by grade, types of programs and services offered, length of 
 school day and school year, graduation and college application rates, 
and teacher turnover rates.
description: Stratified sample. The SASS system of surveys used a set of 
 linked samples. First, a sample of public and private schools was 
 selected. The same sample was used for the School Administrator Survey. 
 Each public school district that administers one or more of the sample 
 schools in the public sector became part of the sample for the Teacher 
 Demand and Shortage Survey. For each sample school, a list of teachers 
 was obtained and a sample was selected for inclusion in the Teacher 
Survey.
description: Datasets: 
DS0: Study-Level Files
DS1: Teacher Demand and Shortage Survey Data
DS2: Teacher Demand and Shortage Survey SAS Transport File
DS3: School Administrator Survey Data
DS4: School Administrator Survey SAS Transport File
DS5: Teacher Survey Data
DS6: Teacher Survey SAS Transport File
DS7: School Survey Data
DS8: School Survey SAS Transport File
description: Public and private schools in the United States.
description: In the 1990-1991 survey, American Indian/Alaska Natives 
 and Asian/Pacific Islanders as well as bilingual and new teachers were 
oversampled.
DOI: 10.3535/ex9-62h-d82
title: Chusquea valdiviensis É.Desv.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/5hl-6hl-0wr
title: Eupatorium urticifolium Reichard

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/dly-yjf-axm
title: Rhamphospermum pubescens (L.) Al-Shehbaz

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3886/icpsr04133
title: Schools and Staffing Survey, 1999-2000: [United States]
title: Archival Version

description: This data collection, sponsored by the National Center for
 Education Statistics (NCES), consists of an integrated set of surveys
 collected from public, private, public charter, and Bureau of Indian
 Affairs (BIA) schools nationwide. This collection provides information
 about teachers and administrators and the general condition of
 America's elementary and secondary schools. Parts 1 and 2, Public and
 Private Teacher Data, provide information about teachers, such as
 teaching field, teaching workload, and teaching experience. Parts 3
 and 4, Public and Private Administrator (Principal) Data, provide
 information about the training, experience, professional background,
 and demographic characteristics of school principals. Parts 5 and 6,
 Public and Private School Data, include information about schools such
 as policies, staffing patterns, student characteristics, programs and
 services offered, and activities related to various school reform
 issues. Part 7, Public District Data, provides information about
 school districts, such as enrollment and teacher counts, graduation
 requirements, and district policies regarding hiring and compensation
 of teachers, accountability reporting, and other measures of recent
reforms.
description: none
description: Stratified sample.
description: Datasets: 
DS0: Study-Level Files
DS1: Teacher Survey Public School Data
DS2: Teacher Survey Private School Data
DS3: Administrator Survey Public School Data
DS4: Administrator Survey Private School Data
DS5: School Survey Public School Data
DS6: School Survey Private School Data
DS7: Public District Data
description: Public and private schools in the United States in
1999-2000.
description: self-enumerated questionnaires; telephone interviewsThis version of the collection is derived from the
NCES's "Schools and Staffing Survey (SASS) 1999-2000 CD-ROM."
DOI: 10.3535/w8r-hdl-j1c
title: Cenchrus setigerus Vahl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/z3c-6sq-eaq
title: Escallonia bifida Link & Otto

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/2qq-ts4-kqh
title: Hedbergia abyssinica (Benth.) Molau

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/ghg-m4e-ev5
title: Escallonia bifida Link & Otto

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/zay-m0z-d8y
title: Hieracium cydoniifolium subsp. cottianum (Arv.-Touv.) Zahn

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/hm9-3vs-mw4
title: Eupatorium L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/nf7-0w3-qrw
title: Cenchrus setigerus Vahl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/70j-53q-5ac
title: Lepidaploa barbata (Less.) H. Rob.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/tpp-qfs-bq9
title: Tilesia G. Mey.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/cbm-pgx-v5q
title: Ziziphus spina-christi (L.) Desf.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/k3n-7zr-8gc
title: Escallonia megapotamica Spreng.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.25549/annis-ouc17142493
title: Lerida, Spain, 1954

description: Lerida, Spain, 1954 June 22.
DOI: 10.15468/dl.jz772k
title: Occurrence Download

description: A dataset containing 1708 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Semnopithecus priam"
 ]
}

 The dataset includes 1708 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001222-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/annis-ouc17143807
title: Cistercian Monastery, Poblet, Spain, 1954

description: Cistercian Monastery, Poblet, Spain, 1954 June 23.
DOI: 10.25549/annis-ouc17145594
title: Cistercian Monastery, Poblet, Spain, 1954

description: Cistercian Monastery, Poblet, Spain, 1954 June 23.
DOI: 10.25549/annis-ouc17141147
title: Cistercian Monastery, Poblet, Spain, 1954

description: Cistercian Monastery, Poblet, Spain, 1954 June 23.
DOI: 10.25549/annis-ouc17142229
title: Cistercian Monastery, Poblet, Spain, 1954

description: Cistercian Monastery, Poblet, Spain, 1954 June 23.
DOI: 10.25549/annis-ouc17141722
title: "Manta, Ecuador," 1935

description: Buildings in Manta, Manabi, Ecuador, 1935 August 25.
DOI: 10.25549/annis-ouc17144905
title: "Manta, Ecuador," 1935

description: Buildings in Manta, Manabi, Ecuador, 1935 August 25.
DOI: 10.25549/annis-ouc17143588
title: Irish coast & Cobh Harbor, Ireland, 1954

description: Irish coast and Cobh Harbor, Ireland, 1954 February 13.
DOI: 10.25549/annis-ouc17146537
title: Irish coast & Cobh Harbor, Ireland, 1954

description: Irish coast and Cobh Harbor, Ireland, 1954 February 13.
DOI: 10.25549/annis-ouc17147560
title: Louvre, Paris, France, 1954

description: Louvre, Paris, France, 1954 February 15.
DOI: 10.15468/dl.q7fe7f
title: Occurrence Download

description: A dataset containing 5072 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hipponix",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 5072 records from 110 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001223-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/annis-ouc17148499
title: Cistercian Monastery, Poblet, Spain, 1954

description: Cistercian Monastery, Poblet, Spain, 1954 June 23.
DOI: 10.25549/annis-ouc17144485
title: Pamplona, Spain, 1954

description: Pamplona, Spain, 1954 June 27.
DOI: 10.25549/annis-ouc17146633
title: Laguardia and Logroño, Spain, 1954

description: Laguardia and Logroño, Spain, 1954 June 28.
DOI: 10.25549/annis-ouc17142729
title: Burgos, Spain, 1954

description: Burgos, Spain, 1954 June 29.
DOI: 10.25549/annis-ouc17141718
title: Burgos, Spain, 1954

description: Burgos, Spain, 1954 June 29.
DOI: 10.25549/annis-ouc17145470
title: Moret-sur-Loing, France, 1954

description: Moret-sur-Loing, France, 1954 February 18.
DOI: 10.25549/annis-ouc17145552
title: Moret-sur-Loing, France, 1954

description: Moret-sur-Loing, France, 1954 February 18.
DOI: 10.25549/annis-ouc17142273
title: Auxerre, France, 1954

description: Auxerre, France, 1954 February 18.
DOI: 10.25549/annis-ouc17148755
title: Auxerre, France, 1954

description: Auxerre, France, 1954 February 18.
DOI: 10.25549/annis-ouc17143754
title: Le Puy-en-Velay, France, 1954

description: Thumbnails of photographs on an index card of Le Puy-en-Velay, France, 1954 February 20.
DOI: 10.25549/annis-ouc17143965
title: Cuenca, Spain, 1954

description: Cuenca, Spain, 1954 May 26.
DOI: 10.25549/annis-ouc17147932
title: Cuenca, Spain, 1954

description: Cuenca, Spain, 1954 May 26.
DOI: 10.25549/annis-ouc17141719
title: Cuenca, Spain, 1954

description: Cuenca, Spain, 1954 May 25-26.
DOI: 10.15468/dl.uehuba
title: Occurrence Download

description: A dataset containing 783 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Semnopithecus schistaceus"
 ]
}

 The dataset includes 783 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001224-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/annis-ouc17141331
title: Cuenca, Spain, 1954

description: Cuenca, Spain, 1954 May 25-26.
DOI: 10.25549/annis-ouc17149417
title: Toledo, Spain, 1954

description: Toledo, Spain, 1954 May 31.
DOI: 10.25549/annis-ouc17145579
title: Buildings in the Nunnery Group, Chichén Itzá, Mexico, 1938

description: Las Monjas, La Iglesia, and other buildings in the Nunnery Group, Chichén Itzá, Yucatán, Mexico, 1938 February 23.
DOI: 10.25549/annis-ouc17145840
title: Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17147260
title: Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17143675
title: Pyramid and ruins, Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17145192
title: Pyramid and ruins, Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17145990
title: Cathedral Notre Dame, Le Puy-en-Velay, France, 1954

description: Cathedral Notre Dame, Le Puy-en-Velay, France, 1954
DOI: 10.25549/annis-ouc17142928
title: Cathedral Notre Dame, Le Puy-en-Velay, France, 1954

description: Cathedral Notre Dame, Le Puy-en-Velay, France, 1954
DOI: 10.25549/annis-ouc17146109
title: Avignon, France, 1954

description: Avignon, France, 1954 February 22.
DOI: 10.25549/annis-ouc17142036
title: Roman ruins, Arles, France, 1954

description: Thumbnails of photographs on an index card of Roman ruins, Arles, France, 1954 February 23.
DOI: 10.25549/annis-ouc17145684
title: St. Trophime, Arles, France, 1954

description: St. Trophime, Arles, France, 1954 February 23.
DOI: 10.25549/annis-ouc17141332
title: Santo Domingo de Silos, Santo Domingo del Calzala, villages between Burgos and Soria, and Najera, Spain, 1954

description: Santo Domingo de Silos, Santo Domingo del Calzala, villages between Burgos and Soria, and Najera, Spain, 1954 June 28 and July 1
DOI: 10.15468/dl.bqfesk
title: Occurrence Download

description: A dataset containing 285 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is CHN.29_1",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Pteruthius melanotis"
 ]
}

 The dataset includes 285 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001225-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/annis-ouc17142236
title: Soria and San Esteban de Gormaz, Spain, 1954

description: Soria and San Esteban de Gormaz, Spain, 1954 July 1.
DOI: 10.25549/annis-ouc17146547
title: Fatima, Portugal, 1954

description: Fatima, Portugal, 1954 July 8.
DOI: 10.25549/annis-ouc17147350
title: Fatima, Portugal, 1954

description: Fatima, Portugal, 1954 July 8.
DOI: 10.25549/annis-ouc17142037
title: Monastery church, Batalha, Portugal, 1954

description: Monastery church, Batalha, Portugal, 1954 July 8 and 23.
DOI: 10.25549/annis-ouc17140394
title: Scenes of San Martín Sacatepéquez, Guatemala, 1934

description: Scenes of San Martín Sacatepéquez, Guatemala, 1934 August.
DOI: 10.25549/annis-ouc17148895
title: Hermitage of Dolores del Cerro, Antigua, Guatemala, 1934?

description: Ruins, la ermita de Nuestra Señora de los Dolores del Cerro, Antigua, Sacatepéquez, Guatemala, 1934?
DOI: 10.25549/annis-ouc17145818
title: Fountains, Antigua, Guatemala, 1934

description: Fountains, Antigua, Guatemala, 1934 August.
DOI: 10.25549/annis-ouc17147290
title: Scenes of San Martín Sacatepéquez, Guatemala, 1934

description: Scenes of San Martín Sacatepéquez, Guatemala, 1934 August.
DOI: 10.25549/annis-ouc17144534
title: Praza da Quintana, Clock tower & Cathedral, Santiago de Compostela, Spain, 1954?

description: Clock tower and rear of Cathedral, on Praza da Quintana, Santiago de Compostela, Spain, 1954?
DOI: 10.25549/annis-ouc17140788
title: St. Trophime, Arles, France, 1954

description: St. Trophime, Arles, France, 1954 February 23.
DOI: 10.25549/annis-ouc17141721
title: St. Trophime, Arles, France, 1954

description: St. Trophime, Arles, France, 1954 February 23.
DOI: 10.25549/annis-ouc17146542
title: St. Trophime, Arles, France, 1954

description: St. Trophime, Arles, France, 1954 February 23.
DOI: 10.25549/annis-ouc17146237
title: Barcelona, Spain, 1954

description: Barcelona, Spain, 1954 February 28.
DOI: 10.25549/annis-ouc17142773
title: Barcelona, Spain, 1954

description: Barcelona, Spain, 1954 February 28 and June 24.
DOI: 10.25549/annis-ouc17149430
title: Barcelona, Spain, 1954

description: Barcelona, Spain, 1954 February 28 and June 24.
DOI: 10.25549/annis-ouc17141869
title: Monastery, Montserrat mountain, Spain, 1954

description: Monastery, Montserrat mountain, Spain, 1954 March 2.
DOI: 10.25549/annis-ouc17143820
title: Moret-sur-Loing, France, 1954

description: Moret-sur-Loing, France, 1954 February 18.
DOI: 10.25549/annis-ouc17144011
title: Moret-sur-Loing, France, 1954

description: Moret-sur-Loing, France, 1954 February 18.
DOI: 10.6084/m9.figshare.31386070
title: <b>Supplementary</b><b> </b><b>Table 5</b>

description: Comparison of either the <i>Listeria monocytogenes</i> (Lm) strain AUF-2 genome or the Lm strain AUF-3 genome with the NCBI reference sequence the Lm strain EGD-e. Comparative analysis of these Lm whole genomes was done on the BV-BRC platform (https://www.bv–brc.org/).
DOI: 10.6084/m9.figshare.31386070.v1
title: <b>Supplementary</b><b> </b><b>Table 5</b>

description: Comparison of either the <i>Listeria monocytogenes</i> (Lm) strain AUF-2 genome or the Lm strain AUF-3 genome with the NCBI reference sequence the Lm strain EGD-e. Comparative analysis of these Lm whole genomes was done on the BV-BRC platform (https://www.bv–brc.org/).
DOI: 10.25549/annis-ouc17143461
title: Palma de Mallorca, Spain, 1954

description: Palma de Mallorca, Spain, 1954 April 6.
DOI: 10.25549/annis-ouc17145472
title: Pyramid and ruins, Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17141752
title: Buildings at Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17148871
title: Buildings at Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17148558
title: Buildings at Uxmal, Mexico, 1938

description: The archaeological site of Uxmal in Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17145650
title: Stela and buildings, Uxmal, Mexico, 1938

description: A stela with hieroglyphics, and buildings in both quadrangles at Uxmal, Yucatán, Mexico, 1938 February 22.
DOI: 10.25549/annis-ouc17140532
title: Church at Tibidabo, near Barcelona, Spain, 1954

description: Church at Tibidabo, near Barcelona, Spain, 1954 February 3.
DOI: 10.25549/annis-ouc17142844
title: Church at Tibidabo, near Barcelona, Spain, 1954

description: Church at Tibidabo, near Barcelona, Spain, 1954 February 3.
DOI: 10.25549/annis-ouc17140382
title: Cathedral, Tarragona, Spain, 1954

description: Cathedral, Tarragona, Spain, 1954 March 11 and June 23.
DOI: 10.25549/annis-ouc17144908
title: Cathedral, Valencia, Spain, 1954

description: Cathedral, Valencia, Spain, 1954 March 18.
DOI: 10.25549/annis-ouc17143987
title: Cathedral, Valencia, Spain, 1954

description: Cathedral, Valencia, Spain, 1954 March 18.
DOI: 10.34740/kaggle/dsv/14917465
title: SRD Dataset
DOI: 10.25549/annis-ouc17147255
title: Monastery church, Batalha, Portugal, 1954

description: Monastery church, Batalha, Portugal, 1954 July 8 and 23.
DOI: 10.25549/annis-ouc17140213
title: Braga, Guimarais, and Viana do Castelo, Portugal, 1954

description: Braga, Guimarais, and Viana do Castelo, Portugal, 1954 July 26.
DOI: 10.25549/annis-ouc17144480
title: Braga, Guimarais, and Viana do Castelo, Portugal, 1954

description: Braga, Guimarais, and Viana do Castelo, Portugal, 1954 July 26.
DOI: 10.5281/zenodo.11557638
title: Discovery of small molecules as HPV16 E6 protein inhibitors against cervical cancer: An in silico and biophysical approach

description: All the input files used for molecular docking and molecular dynamics simulations performed with HPV16 E6 protein to find potential inhibitors.

Considering the critical role of high-risk HPV E6 protein in malignant tumorigenesis, it is widely recognized as a therapeutic target to develop novel and effective treatments for CC. In this work, we explored computational methods to discover new E6 inhibitors with potential interest in CC therapy. Using the E6 protein structure published in the Protein Data Bank (4XR8), molecular docking studies and molecular dynamics simulations were performed to predict the binding energy and orientation of synthetic compounds at the E6-p53 binding site. For experimental studies, the recombinant MBP-E6 protein was expressed from the Escherichia coli host, and affinity chromatography was applied to obtain highly pure protein. Circular dichroism confirmed that the target protein adopts an α-helical, random coil, and β-sheet structure. Finally, a thermal shift assay was used to access potential protein-ligand interactions.