cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/7xvvx-1et26
title: CheA-Ge8-L2

description: This strain lacks a chromosomal CheA, and carries plasmid pj207 containing a CheA with a trimer of the silver peptide in linker region  two.
description: <strong>Tilt Series Date:</strong> 2012-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Dan Feldheim and Carly Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB plus Kanamycin, induced with 14nM IPTG for one hour, penicillin treated for an additional hour</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-16/rawdata/</p>CheAmutantpart20007.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-16/rawdata/CheAmutantpart20007.mrc"
        > <i class="download icon"></i> Download </a></p>

        CheAmutantpart20007_part128_74.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-22-16/3dimage_17953/CheAmutantpart20007_part128_74.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2vg3dr
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Acorus calamus",
 "Country is Türkiye",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047669-250811113504898/datasets/export for details.
DOI: 10.22002/y82kr-cp019
title: Agrobacterium delta rem

description: Agrobacterium tumefaciens non-motile delta rem mutant
description: <strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-48/rawdata/</p>delta_rem0035.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-48/rawdata/delta_rem0035.mrc"
        > <i class="download icon"></i> Download </a></p>

        delta_rem0035_part121_7.rec,  Reconstruction (Pixel Size 2.24 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-48/3dimage_41049/delta_rem0035_part121_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rh75ac
title: Occurrence Download

description: A dataset containing 13402 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is South America",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Triatoma sordida, Triatoma infestans, Triatoma guazu, Triatoma guasayana, Pastrongylus megistus, Geniculatus)"
 ]
}

 The dataset includes 13402 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047667-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.sf4sth
title: Occurrence Download

description: A dataset containing 19313 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Lithodora fruticosa"
 ]
}

 The dataset includes 19313 records from 160 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047665-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/n82e2-6jn14
title: UU 2803- A413T delta P1P2

description: E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB medium, lysed with penicillin for 1 hour</p>
<strong>Sample Preparation:</strong> plunge frozen in R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-29/rawdata/</p>2803atlas10029.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-29/rawdata/2803atlas10029.mrc"
        > <i class="download icon"></i> Download </a></p>

        2803atlas10029_part130_17.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-29/3dimage_20555/2803atlas10029_part130_17.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9sfev-r6k30
title: Pseudomonas aeruginosa mut

description: Quadruple mutant for polyP synthesis
description: <strong>Tilt Series Date:</strong> 2014-02-09</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Lisa Racki</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth 3hours into starvation</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
  etl2014-02-09-2_slicer6528.jpg: Chemoreceptor etl2014-02-09-2_slicer6534.jpg: filament etl2014-02-09-2_slicer6536.jpg: Unknown filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-09-2/rawdata/</p>quadmut_t3_20002.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-09-2/rawdata/quadmut_t3_20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        quadmut_t3_20002_part121_1.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-09-2/3dimage_49276/quadmut_t3_20002_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jzshv-9mr34
title: M-MuLV 43D infected MEFs

description: M-MuLV 43D wildtype
description: <strong>Tilt Series Date:</strong> 2014-03-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> pipeline raptor</p>
<strong>Collaborators and Roles:</strong> M-MuLV infected cells provided by Hung Fan (UC Irvine)</p>
<strong>Sample Preparation:</strong> Whole MEFs infected with M-MuLV 43D were cultured on Au grids, blotted manually and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-03-29-13/rawdata/</p>14mar29_C182-1_MLV43D_3T3_AT1BT2_0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-03-29-13/rawdata/14mar29_C182-1_MLV43D_3T3_AT1BT2_0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        14mar29_C182-1_MLV43D_3T3_AT1BT2_0004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2014-03-29-13/3dimage_40582/14mar29_C182-1_MLV43D_3T3_AT1BT2_0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/08bf4-v0t07
title: HIV capsid purified trim5 alpha

description: 41000 6df, with slit 91/A2
description: <strong>Tilt Series Date:</strong> 2013-12-13</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 91.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod and SIRT reconstruction</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified recombinant trim5 alpha decorated with decorated HIV cores</p>
<strong>Sample Preparation:</strong> B grid</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-28/rawdata/</p>Trim5alpha41000df60008.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-28/rawdata/Trim5alpha41000df60008.mrc"
        > <i class="download icon"></i> Download </a></p>

        Trim5alpha41000df60008_part121_67.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-28/3dimage_36069/Trim5alpha41000df60008_part121_67.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b65jc-kx470
title: Agrobacterium delta rem

description: Agrobacterium tumefaciens non-motile delta rem mutant
description: <strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-33/rawdata/</p>delta_rem0020.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-33/rawdata/delta_rem0020.mrc"
        > <i class="download icon"></i> Download </a></p>

        delta_rem0020_full.rec,  Reconstruction (Pixel Size 2.24 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-33/3dimage_41021/delta_rem0020_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/geqjp-1tx19
title: Agrobacterium tumefaciens

description: Agrao VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-08</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-74/rawdata/</p>agro_vg31010002.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-74/rawdata/agro_vg31010002.mrc"
        > <i class="download icon"></i> Download </a></p>

        agro_vg31010002_full.rec,  Reconstruction (Pixel Size 0.0 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-74/3dimage_48933/agro_vg31010002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gtefh-qzx46
title: HeLa

description: Synchronized, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2014-01-06</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> With Doug Mckay and Katherine Ullman</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA NUP 153+ Thymidine 2mM. 14 hour after release</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-48/rawdata/</p>midbody20001.mrc,  Tilt Series (Pixel Size 0.6 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-48/rawdata/midbody20001.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody20001_part121_7.rec,  Reconstruction (Pixel Size 2.4 nm), 156.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-48/3dimage_50463/midbody20001_part121_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q74r6-m3k84
title: E. coli N050

description: Sandwich fusion to sfGFP. Wild Type MreB (no point mutations)
description: <strong>Tilt Series Date:</strong> 2014-03-27</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli </p>
<strong>Strain:</strong> NO50</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Josh Schaevitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown at 37 to OD600 = 0.3 then plunge frozen.</p>
<strong>Sample Preparation:</strong> Plunge Frozen</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-03-27-12/rawdata/</p>032714_NO500019.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-03-27-12/rawdata/032714_NO500019.mrc"
        > <i class="download icon"></i> Download </a></p>

        032714_NO500019.rec,  Reconstruction (Pixel Size 0.96 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-03-27-12/3dimage_40556/032714_NO500019.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2crj9-jey73
title: HIV capsid

description: Box C, 0.5 exposure, 152, 41000 mag 3 defocus, slit 20 eV, search track 13500
description: <strong>Tilt Series Date:</strong> 2013-12-05</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 152.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod, need to reconstruct with SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified HIV cores</p>
<strong>Sample Preparation:</strong> Box C</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-56/rawdata/</p>test_hivcapsid00023.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-56/rawdata/test_hivcapsid00023.mrc"
        > <i class="download icon"></i> Download </a></p>

        test_hivcapsid00023_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-56/3dimage_35597/test_hivcapsid00023_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/86dg8-dd128
title: Agro C58

description: Wild type Agrobacterium C58
description: <strong>Tilt Series Date:</strong> 2013-09-13</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on agar plates for 2 days on Au grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-43/rawdata/</p>C58_au_34k0044.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-43/rawdata/C58_au_34k0044.mrc"
        > <i class="download icon"></i> Download </a></p>

        34k0044_cut_full.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-43/3dimage_49140/34k0044_cut_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t40dw-51e17
title: E. Coli delta53

description: Mutant MreB with sfGFP in sandwich position. WT MreB is replaced at the original locus with this fusion.
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia Coli </p>
<strong>Strain:</strong> delta53</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Josh Schaevitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 celsius to OD 600 ~ 0.3</p>
<strong>Sample Preparation:</strong> Blotted manually from rear in vitrobot at 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-01-39/rawdata/</p>040114_delta53_0023.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        040114_delta53_0023_part131_17.rec,  Reconstruction (Pixel Size 1.68 nm), 861.2 MB  
        
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