cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.1594/pangaea.965969
title: Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with different aprotinin treatments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of residual total protease activity by spectroscopy after addition of the protease inhibitor aprotinin.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965970
title: Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with different soybean trypsin inhibitor (SBTI) treatments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the residual total protease activity by spectroscopy after addition of the protease inhibitor Soybean Trypsin Inhibitor.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965971
title: Digestion rate in larval Strongylocentrotus purpuratus during laboratory experiments with low protease inhibitor treatments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the in vivo digestion rate determined by fluorescence microscopy under the influence of protease inhibitors.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965972
title: Digestion rate in larval Strongylocentrotus purpuratus during laboratory experiments with high protease inhibitor treatments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the in vivo digestion rate determined by fluorescence microscopy under the influence of 10-fold concentrated protease inhibitors.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965973
title: Fluorescence intensity in the larval Strongylocentrotus purpuratus midgut during laboratory experiments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the decrease of the fluorescence signal during in vivo digestion of Rhodomonas sp.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965976
title: Immoblization of Rhodomonas sp. during laboratory experiments with different trypsin treatments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the motility of Rhodomonas sp. using different trypsin concentrations.
DOI: 10.1594/pangaea.965981
title: Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with aprotinin treatment over time

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the required pre-incubation time for the maximum efficacy of the aprotinin inhibitor when using in the total protease activity measurements.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965982
title: Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with soybean trypsin inhibitor (SBTI) treatment over time

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the required pre-incubation time for the maximum efficacy of the soybean trypsin inhibitor when using in the total protease activity measurements.-----The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
DOI: 10.1594/pangaea.965983
title: Trypsin activity during laboratory experiments with different protease inhibitor and pH treatments

description: Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
description: Determination of the effect of commercially aquired trypsin on the food (Rhodomonas. sp.) in filtered seawater (FSW) and artificial seawater (ASW) under consideration of different pH values and protease inhibitors
DOI: 10.1594/pangaea.967439
title: 3D multichannel seismic reflection processed data (P-Cable system with GI Gun) of RV POSEIDON during cruise POS515, Calabrian Arc, Mediterranean Sea

description: The data are a processed and interpolated P-Cable 3D seismic data cube. The data were collected during expedition POS515 with the research vessel POSEIDON (Riedel et al., 2017) in the Mediterranean Sea, Calabrian Arc offshore southern Italy (map under further details). Seismic source was a Generator-Injector airgun fired in harmonic GI mode with generator and injector volume of 105 cubic inches. The source was towed at a nominal depth of 2 m beneath the sea surface. We deployed a P-Cable system made from 12 parallel streamers (each 12. 5 m in length). Details on the towed geometry are given in Figure 30 of the cruise report (Riedel et al., 2017). The P-Cable data were acquired over the course of 6 days during POS515 starting from 13:45 on July 5, 2017 until 12:06 on July11, 2017 (all CET). Processing included: 3D geometry application, trace editing, static time corrections, band-pass filtering, sorting to common midpoint gathers, normal moveout correction (1520 m/s velocity ), and stacking. The stacked 3D cube was then interpolated to fill any empty bins. Afterwards, Stolt time migration (1550 m/s) was performed, first inline, then crossline direction. The dataset has a horizontal resolution of 3.125 m (bin size) and a dominant frequency of ~100 Hz.
description: The data are provided in SEG-Y format.3D P-Cable corner points:inline: 3606 crossline: 3200 Lat: 38.15668 Lon: 17.63030inline: 3606 crossline: 5850 Lat: 38.23114 Lon: 17.63577inline: 5117 crossline: 5850 Lat: 38.23359 Lon: 17.58194Number of inlines: 3606 - 5117 (total 1512)Number of crosslines: 3200 - 5850 (total 2651)The sampling rate: 0.001 sTime extracted: 2.34 s - 3.54 s (1201 samples total)Lagtime: (580 ms): 109-110The following header locations can be used for accessing coordinates:Bin-size: 3.125 mX-bin coordinate: byte 81-84Y-bin coordinate: byte 85-88Inline-number: 17-20Crossline-number: 21-24