DOI: 10.5061/dryad.45058jq
title: Data from: Variation in ecophysiological traits might contribute to ecogeographic isolation and divergence between parapatric ecotypes of Mimulus aurantiacus

description: Many forms of reproductive isolation contribute to speciation, and early
 acting barriers may be especially important, because they have the first
 opportunity to limit gene flow. Ecogeographic isolation occurs when
 intrinsic traits of taxa contribute to disjunct geographic distributions,
 reducing the frequency of inter‐taxon mating. Characterizing this form of
 isolation requires knowledge of both the geographic arrangement of
 suitable habitats in nature and the identification of phenotypes involved
 in shaping geographic distributions. In Mimulus aurantiacus, red‐ and
 yellow‐flowered ecotypes are incompletely isolated by divergent selection
 exerted by different pollinators. However, these emerging taxa are largely
 isolated spatially, with a hybrid zone occurring along a narrow region of
 contact. In order to assess whether responses to abiotic conditions
 contribute to the parapatric distribution of ecotypes, we measured a
 series of ecophysiological traits from populations along a transect,
 including drought sensitivity, leaf area, and the concentrations of
 vegetative flavonoids. In contrast to the abrupt transitions in floral
 phenotypes, we found that ecophysiological traits exhibited a continuous
 geographic transition that largely mirrors variation in climatological
 variables. These traits may impede gene flow across a continuous
 environmental gradient, but they would be unlikely to result in ecotypic
 divergence alone. Nevertheless, we found a genetic correlation between
 vegetative and floral traits, providing a potential link between the two
 forms of isolation. Although neither barrier appears sufficient to cause
 divergence on its own, the combined impacts of local adaptation to abiotic
 conditions and regional adaptation to pollinators may interact to drive
 discontinuous variation in the face of gene flow in this system.
description: drought_scores_rawThese are raw drought scores from the terminal drought experiment described in M&M section 2.3.droughtdata_mainThis file contains the drought sensitivity metric calculated from raw drought scores as described in M&M section 2.3 (both untransformed and transformed). It also contains leaf area measurements for each individual (untransformed and transformed).leaf_traits_mainThis file contains leaf ecophysiology traits, vegetative anthocyanin and resin in both stressed and unstressed treatments. Untransformed data are included for all variables, and transformed for those required. See M&M section 2.4.ecophys_traits_by_familyThis file contains summarized drought, leaf area, vegetative anthocyanin and resin data, with individual replicates averaged by family. See M&M section 2.5.pairwise_fstThis data file contains a square matrix of Fst values between each population in the ecophys experiments. See M&M section 2.5. These data were originally estimated and presented in Stankowski et al 2015 (Evolution 69:3054-3068).population_climateThis data file contains population level information about climatological variables collected from WORLDCLIM. See M&M section 2.5.biomassThis data file contains above and below-ground biomass data. See M&M section 2.6.myb2_antho_cosegregationThis data file contains myb2 genotypes, and visible stem and leaf anthocyanins for a population of f2's from a cross between red and yellow parents.