cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0js9p8m
title: CIL:10927, Felis catus, myelinating Schwann cell, peripheral neuron. In Cell Image Library

description: This electron micrograph is a cross section through a portion of an axon and its associated myelin wrap from the cochlear nerve of the cat. This high magnification view showcases the regular periodicity with which the myelin wraps the axon. Each dense line represents where the internal surfaces of the apposed Schwann cell membranes are in contact. The faint intraperiod lines are where the outer surfaces of the two membranes are in contact. This micrograph, by Enrico Mugnaini is Figure 15 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D., and accompanies a lower magnification micrograph shown in Fig. 14 of this chapter. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide
Data qualification: Processed
DOI: 10.6075/j0gf0s9t
title: CIL:10917, Rattus, Mus musculus, transitional epithelial cell. In Cell Image Library

description: Preparation: osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue; whole mounted tissue
Item type: transmission electron microscopy (TEM); scanning electron microscopy (SEM)
Imaging mode: illumination by electrons; detection of electrons; secondary_electron imaging
Parameter imaged: electron density
Visualization methods: stain with broad specificity
Data qualification: Processed
description: Transitional epithelial cells of the bladder. Upper panel is an electron micrograph from thin sectioned superficial cells of the uroepithelium in the rat; lower panel shows a scanning electron micrograph of the luminal surface of mouse bladder. Both images reveal rigid plaques (see brackets, top panel)along the surface of the epithelial tissue separated by more flexible interplaque regions. In the lower panel, the polygonal plaques appear sunken, while the more flexible interplaque regions appear as ridges or folds. Figures 11 (upper panel, courtesy of Marian Hicks) and 12 (lower panel, courtesy of Linda Malick) from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0kw5drd
title: CIL:10914, Macaca mulatta, retinal cone cell, retinal bipolar neuron, horizontal cell. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: Freeze fracture images of synaptic junctions in the retina of Macaca mulatta. The electron micrograph in the top panel shows two invaginating synapses between cone and horizontal cells in the outer plexiform layer of the retina. Arrows indicate regions of particle aggregation on the presynaptic side, candidate channels associated with vesicle release), the stars point to exposed internal specializations, candidate receptor sites, on the postsynaptic plasma membrane. The micrograph in the lower panel shows two postsynaptic processes of a dyad synapse in the inner plexiform layer. Arrows indicate aggregations of particles associated with the e-face of the freeze fracture replica. Upper micrograph reprinted from E. Raviola and N. B. Gilula, J. Cell Biol, 1975); lower micrograph courtesy of E. Raviola and G. Raviola. Figure 10 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: With the freeze-fracture technique, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface. The intramembranous particles represent integral membrane proteins.
DOI: 10.6075/j0nk3cv3
title: CIL:10909, Rattus, Homo sapiens, ciliated epithelial cell. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: Freeze fracture images of ciliated epithelial cells, Figures 8 (top) and 9 (bottom 2 panels) from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Figure 8 shows the P-face of ciliated epithelial cells from the ductus efferens of the rat, highlighting the "ciliary necklace" of imtramambrane particles the form several circumferential rows around the base of the shaft of each cilium. Figure 9 reveals linear arrays of particles that can be found on a small percentage of columnar epithelial cells in the colon and rectum of primates, shown here on the brush border of surface columnar cells from human rectal mucosa. A PDF copy of the accompanying chapter from Don Fawcett's The Cell is available on the ASCB's BioEDUCATE website.
description: With the freeze-fracture technique, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface. The intramembranous particles represent integral membrane proteins.
DOI: 10.6075/j0r78d14
title: CIL:10973, Chinchilla, epithelial cell. In Cell Image Library

description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
description: Transmission electron micrograph of a section of Chinchilla epididimus showing the highly lobed nucleus seen in Principal sells of the epididymal epithelium characteristic of many mammals. Figure 111 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0xd10gm
title: CIL:10971, Homo sapiens, neutrophil. In Cell Image Library

description: Transmission electron micrograph of a section of a human polymorphonuclear neutrophil from circulating blood. Neutrophil nuclei are often lobed, as seen in the electron micrograph and light micrograph at lower right. Figure 110 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j06q1w14
title: CIL:10978, Cavia porcellus, plasma cell. In Cell Image Library

description: Transmission electron micrograph illustrating the accumulation of dense heterochromation that accompanies terminal differentiation in may cells. Shown here is a section through a polychromatophilic erythroblast from guinea pig bone marrow. The exclusion of heterochromatin from nuclear pore regions is clearly seen. Figure 115 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed
DOI: 10.6075/j0v986v1
title: CIL:10954, skeletal muscle cell. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: Quick-frozen deep-etched preparation of skeletal muscle tissue shows the muscle fiber and associated extracellular components. The basement membrane (labeled basal lamina) lies adjacent to the undulating sarcolemma. Myofilaments of the muscle fiber are composed of actin and myosin filaments. Image by J. Heuser, Figure 30 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0q52ngk
title: CIL:10953, skeletal muscle cell. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: Sarcolemma of skeletal muscle prepared by a quick-freeze deep-etch method also shows the basement membrane (lamina externa, labeled here as the basal lamina) associated with the muscle fiber. This method reveals a basement membrane composed of a meshwork of fibers, some of which contact the cell membrane. The larger fibrils outside of the lamina externa (to the far lower right of the image) are composed of collagen. Image by John Heuser, Figure 29 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0x34w8q
title: CIL:10946, Siphonaptera, epithelial cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: electron-dense stain
Data qualification: Processed
description: This electron micrograph shows the basal lamina along the midgut epithelium of the flea. Unlike the vertebrate basal lamina, which appears as a homogenous layer, the basal lamina in insects often appears striated. The inset panel offers a different view of the basal lamina in a section that is parallel to the base of the epithelium. In this view, the basal lamina is organized as a mosaic of elongated dense bodies with rows of smaller dense granules interspersed. Image by Susumu Ito, Figure 24 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j08w3c3x
title: CIL:10944, Homo sapiens, corneal endothelial cell. In Cell Image Library

description: This electron micrograph shows the endothelium and the underlying Descemet's membrane of the human cornea. Descemet's membrane is an unusually thick and structurally specialized example of basal lamina, secreted by the adjacent endothelial cells. The collagen of this layer may exhibit an organized array of evenly spaced nodes connected by radiating fibrils to give a lattice-like appearance. Image by T. Kuwabara, Figure 23 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: electron-dense stain
Data qualification: Processed
DOI: 10.6075/j08051dm
title: CIL:10942, Homo sapiens, corneal epithelial cell. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: electron-dense stain
Data qualification: Processed
description: This electron micrograph shows the basal lamina as a thin gray moustache following parallel along the basal membrane of epithelial cells from human corneal epithelium. In this tissue, the basal lamina, which is secreted by the epithelial cells, is at the interface of the epithelial layer and Bowman's layer, which is composed of condensed collagen. Image by Toichiro Kuwabara, Figure 22 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j05b019w
title: CIL:10908, Didelphinae, sperm. In Cell Image Library

description: This freeze fracture micrograph shows a region of membrane from the midpiece of opossum Didelphis virginiana sperm. This region of the sperm is notable for the repeating longitudinal bands composed of 3-5 intramembranous particles (IMPs). This micrograph by Gary Olson is Figure 7 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: With the freeze-fracture technique, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface. The intramembranous particles represent integral membrane proteins.
DOI: 10.6075/j03b5xzf
title: CIL:10919, Rattus, transitional epithelial cell. In Cell Image Library

description: With the freeze-fracture technique, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface. The intramembranous particles represent integral membrane proteins.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: Freeze fracture image of the E-face of rat bladder lumenal membrane. The lumenal surface of these epithelial cells is composed of plaques separated by interplaque regions. Within the plaques are hexagonal arrays of subunits (about 12nm, with center-to-center spacing of 15.6nm) on the E-face of the membrane. The inset panel shows these subunits at higher magnification, prepared by deep-etching rather than fracturing after freezing. At higher magnification, each subunit within the plaque appear to be composed of a ring of six particles surrounding a channel. These plaques are distinguished from gap junctions (found on the P-face of membranes) by their location on their position instead on the E-face. These micrographs courtesy of Nicholas Severs and Marian Hicks, Figure 13 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0sb44jc
title: CIL:10907, Didelphis marsupialis, sperm. In Cell Image Library

description: This electron micrograph shows a cross section through the midpiece of opossum sperm. Filamentous cytoplasm underlies a scalloped membrane with regular variations in thickness, which gives it a corrugated appearance. Aat the center is an arrangement of dense fibers and microtubule doublets. Figure from page 20, Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Data qualification: Processed
DOI: 10.6075/j07h1hbv
title: CIL:10903, Cavia porcellus, sperm. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
description: With freeze-fracture, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface.
description: This image, produced by the freeze-fracture technique, shows regional specialization in the structure of mammalian sperm. The P-face of the plasma membrane of a guinea pig sperm tail can be divided into three zones, each with different distributions of intramembranous particles (reflecting integral membrane proteins). This image was first published in Friend and Fawcett (1974) J. Cell Biol. 63:641-64, and reprinted as Figure 5 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0416x3t
title: CIL:35900, Saccharomyces cerevisiae. In Cell Image Library

description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of electrons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. 
 
 TEM1 is a GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis. 
 
These phase,gfp images are part of an image group that ranges from CIL:35900-35919. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
DOI: 10.6075/j0g160w2
title: CIL:10208, Rattus, multipolar neuron. In Cell Image Library

description: This multi-layer image shows the spatial relationship between filamentous actin (red) and microtubule array (green) in cultured hippocampal neurons, grown for 1 day in vitro. Actin staining (with rhodamine phalloidin) highlights the growing tips and filopodial extensions along axons and dendrites, while microtubule staining reveals the stable shafts of these processes. Some nonneuronal cells may also appear in the field. Neurons at 1, 3 and 5 days in vitro are represented in this image group.

Detailed Methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, 37°C, 15 minutes), permeabilized (0.25% Triton, 7 minutes) and immunostained for tubulin (monoclonal DM1A, Sigma, with Alexa 488 conjugated secondary, Molecular Probes, excitation, 494, emission, 519) and rhodamine-conjugated phalloidin (Molecular Probes, excitation, 540, emission, 565). Fluorescent and phase images were acquired with a Leica DMRA microscope with a mercury arc lamp, a 40X lens (HCX PL Fluotar, NA 0.75), Leica GFP filter set (excitation, BP 470/40; dichromatic mirror, 500, suppression filter, BP 525/50); Leica N3 filter set (excitation, BP546/12; dichromatic mirror, 565, suppression filter, BP 600/40), Photometrics CoolSnap ES CCD camera and MetaMorph software.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data