DOI: 10.5061/dryad.cnp5hqcc1
title: Data from: Understanding the systematic implications of pollinium morphology of Aeridinae (Orchidaceae)

description: Molecular phylogenetic studies have suggested major clades in the subtribe
 Aeridinae of Orchidaceae. However, systematic
 morphological charaters is still lacking. We carried out this
 study on the pollinium morphologies of subtribe Aeridinae with
 emphases on the aperture types and the pollinium sculpture to
 find out their systematic signification in light of a
 molecular phylogeny. The results indicate that
 most species have cleft type and few with
 porate type or none. The shapes of most pollinium are
 flat spheroid but not constant at genera levels. The
 longth scope of the pollinium is 204-2606 um and the width scope
 is 178-1524 um. The long and wide pollinium are easily found in more
 primitive groups. The short and narrow pollinium are commonly found in
 more evolutionary groups. Shapes and sizes of individual units of
 pollinium vary from uniformity to
 difference with few
 exceptions: isometrical polygons in
 Phalaenopsis of Phalaenopsis clade,
 Vanda clade and Aerides clade and
 irregular shape and different sizes in rest
 samples. Individual units of most pollinium are
 tetrads and individual pollen grains are easy to
 distinguish within the tetrad. The degree of
 discriminability of individual pollen grains is
 constant at clades level and changes following a tendency except Aerides
 rosa: unrecognizable in Chiloschista clade - easily
 distinguished in Phalaenopsis clades,
 Vanda, Aerides and Gastrocohilus clades - partly distinguished in Trichoglottis and Cleisostoma clade. Exine sculpture characters are consistent in each clades except Cleisostoma clade and Phalaenopsis clade. It has a somewhat clear changing tendency in consist with the molecular phylogeny with few exceptions: from negative reticulum of Phalaenopsis clade - reticulum of Chiloschista clade - levelled psilate surface of Vanda and Aerides clades – bulgy with psilate surface in Gastrocohilus and Trichoglottis clades - two types in Cleisostoma clade. Conclusionarily, these pollinium characters are not systematic but useful characters in determining major systematic groups in subtribe Aeridinae.
description: <strong><em>M</em></strong><strong><em>olecular phylogeny of pollinium samples</em></strong>—5 DNA sequences including ITS, <em>atpI-H</em>, <em>matK</em>, <em>psbA-trnH </em>and<em> trnL-F </em>were downloaded from Genebank and an alternative species from the same genus was used when DNA sequences of the pollinium samples were unavailable (Appendix 2). Sequence alignments were made with Clustal X (Thompson et al. 1997) and refined manually with BioEdit (Hall 1999). Phylogenetic analyses were performed under maximum parsimony (MP). Some unavailable sequences in combined data analyses were treated as missing. Parsimony analyses were conducted in PAUP* 4.0b10 (Swofford 2002), and all characters were equally weighted and unordered. The test settings included 1000 replications of random addition sequence and heuristic search with TBR branch swapping. To estimate the clade support, heuristic searches were completed for 1000 bootstrap replicates with 10000 random sequence additions (holding one tree at each step) (Harbaugh and Baldwin 2007). The datasets were also analyzed under Bayesian inference using MrBayes v.3.2.3 (Ronouist and Huelsenbeck 2003). The best-fit model were selected with Modeltest 3.06 (Posada and Crandall 1998). From a random starting tree, a Bayesian analysis was run for 1000000 generations with four simultaneous chains and sampling trees every 100 generations. The first 300 samples for each run were discarded as burn-in to ensure that the chains reached stationarity. Majority-rule consensus trees were constructed on those trees sampled after generation 30000.
description: # Molecular phylogenic tree
 [https://doi.org/10.5061/dryad.cnp5hqcc1](https://doi.org/10.5061/dryad.cnp5hqcc1) ## Description of the data and file structure The file can be used to build the molecular phylogenetic tree. The file (5_DNA_GE_NES.nex) includes 5 DNA sequences including ITS, *atpI-H*, *matK*, psbA-trnH, and trnL-F downloaded from Genebank.