cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25549/examiner-c44-76146
title: Change name of dog, 1951

description: 2 images. Change name of dog, 13 September 1951. Dog's name "Corky"; Henry R. East (trainer and owner of dog).
DOI: 10.25549/examiner-c44-76540
title: Kiddie Korner, Pasadena, 1951

description: 2 images. Kiddie Korner, Pasadena, 14 September 1951. Anna Moore -- 7 years; David Swaim -- 5 years; Billy More -- 9 years.; Caption slip reads: "Photographer: Sewell (vaction relief). Date: 1951-09-14. Reporter: Swaim. Assignment: Kiddie Korner Pasadena. l. to r., 11/12: Anna Moore, 7, David Swaim, 5, and Billy Moore, 9, whiling away time with comic books in Kiddie Korner of super market. Recently installed innovation is so popular with both children and shopping mothers it is going to be enlarged, painted with child murals".
DOI: 10.25549/examiner-c44-76700
title: J.B. Finch Furniture Store opening, 1952

description: 4 images. J.B. Finch Furniture Store opening, 09 July 1952. J.B. Finch (owner); Phyllis Lowell (model); M.W. Mears (ad director).
DOI: 10.25549/examiner-c44-76335
title: Mexican Independence celebration, 1951

description: 7 images. Mexican Independence celebration, 15 September 1951. Mayor Fletcher Bowron; Consul General Salvador Duhart M.; Francisco Gonzalez de la Vega, Mexican Attorney General; Alicia Marquez; Raquel Vasquez; Luz Elena Ortega; Tito Guizar.
DOI: 10.25549/examiner-c44-76405
title: Cop stabbed, 1951

description: 2 images. Cop stabbed, 17 September 1951. Officer Jack Romero (stabbed in face at Broadway and Ord).; Caption slip reads: "Photographer: McCarthy. Reporter: Benam. Assignment: Cop stabbed. 19/20: officer Jack Romero, stabbed in face at Broadway and Ord".
DOI: 10.25549/examiner-c44-56240
title: Swimming, 1958

description: 72 images. Swimming, 17 June 1958. Paula Jean Myers; Gary Tobian. Foton rolls. "Sports". (Sleeve reads: S-12339).
DOI: 10.25549/examiner-c44-76543
title: Ship arrival from Korea, 1951

description: 6 images. Ship arrival from Korea, 11 September 1951.
DOI: 10.25549/examiner-c44-76557
title: Pickets at El Segundo, 1951

description: 4 images. Pickets at El Segundo, 18 September 1951. Pickets march on railroad tracks to prevent train full of supplies from reaching aircraft plant.
DOI: 10.25549/examiner-c44-76651
title: Warner Brothers fire, 1952

description: 44 images. Warner Brothers fire, 9 July 1952.
DOI: 10.25549/examiner-c44-41845
title: Brush fire at little Santa Anita Canyon, 1952

description: 2 images. Brush fire at little Santa Anita Canyon, 2 June 1952. General views.; Caption slip reads: "Photographer: Snow. Reporter: Bickmore. Assignment: Brush fire, Little Santa Anita Canyon. General views".
DOI: 10.25549/examiner-c44-41682
title: Insurance queen, 1952

description: 6 images. Insurance queen, 4 June 1952. Jody Lawrence (Queen); Dee B. Willcuts (Insurance Association); Walter H. Bennett (of New York) National Association of Insurance Agents' General Counsel).
DOI: 10.25549/examiner-c44-10300
title: Butane truck fire, 1951

description: 4 images. Butane truck fire, May 28, 1951. Firemen spraying water on butane truck at San Fernando Road near town of San Fernando.
DOI: 10.25549/examiner-c44-10483
title: Civilian defense (airport bonds stunt), 1951

description: 19 images. Civilian defense (airport bonds stunt), May 22, 1951. Red Cross.
DOI: 10.25549/examiner-c44-40707
title: Baja California ...General views, 1952

description: 46 images. Baja California ...General views, 02 January 1952. Governor Alfonso Garcia Gonzales, sopy; Javier Hernandez, at canal in Mexicali's Pueblo Nuevo section; Mayor Salvador Sierra Cera, Tijuana; Mr. and Mrs. Vic Chaseley, American tourists; Freddie Chaseley -- 4 1/2 ; Mikie Chaseley -- 1.; Caption slip reads: "Photographer: Snow. Reporter: Baker. Assignment: Baja California. Future. 11-12-3-4: cotton fields south of Mexicali. 27-28: Mexicali--San Luis, Sonora Highway, one of new highways in state. 5: Site of new $750,000 Mexicali railroad depot, 3 miles south of town. 6-33: Jabanera del Pacifico, biggest cotton products plant in Baja California. 31-32: Javier Hernandez takes water out of canal in Mexicali's Pueblo Nuevo section. 45-46: Old and new in Mexicali schools. New red-brick, modern Juan Valdez Castillo school foreground, old 1-room frame structure in background. 34, 9, 120: Mound of cottonseed that will be ground into cottonseed oil at jabanera del Pacifico plant, Mexicali. 21, 22: Governor's palace at Mexicali. 23: General view of Mexicali looking north from new 180-ft. high water tower. 24-43: Old and New in Mexicali water systems. Trenches for pipes on left. Old canal on right. 44-75: Workmen work on pipes for new Mexicali water system in Pueblo Nuevo section. New tank in background".; Caption slip continues: "Photographer: Baker... 36: Miguel Aleman Hospital, Tijuana. 85-86: Looking north on Avenida Revolucion, Tijuana's main street. 69-70: American tourists in arcade (Tijuana) l to r: Vic Chaseley, 4 1/2; Mrs. Chaseley; Mikie Chaseley, 2. 20-21: Mexicali Beer Brewery bottle assembly line. Bottles being filled and capped. 87: New 3-story office building going up at Juarez Ave. and Azueta Street, Mexicali. 88: Presidente Miguel Aleman bridge, Mexicali. 52: Looking east on busy Juarez Avenue, Mexicali. 17: Mexican border at Mexicali. 127-128: Mayor Salvador Sierra Vera, Tijuana. 59-60-35: Loading Compania Mexicana Aviacion plane at Tijuana's new airport. 84: Tourists in curio store in Tijuana. 18: New airport at Tijuana. 25-26: President Miguel Aleman Grammar School, Mexicali, newest school in Mexicali. 19: Old thatched roof outdoor school in foreground, new Presidente Miguel Aleman School background, Mexicali".; Other caption slip reads: "Assignment: Baja California Story by George Snow. Copy: Gov. Alfonso Garcia Gonzales, Northern territory, Baja California. (To go with layout negs. by George Snow)".
DOI: 10.25549/examiner-c44-40657
title: Cats, 1952

description: 4 images. Cats, 31 January 1952. Jennie Shelton; "1st Edition" Tripple Champion -- 2 years old cream Persian.; Caption slip reads: "Photographer: Paegel. Date: 1952-01-31. Reporter: Markson. Assignment. For Sunday.27-28-9-10: Jeannie Shelton and Triple Champion, '1st edition' -- Two year old cream. Persian."
DOI: 10.25549/examiner-c44-41735
title: Tournament of Roses floats, 1952

description: 8 images. Tournament of Roses floats, 01 January 1952. American Legion Post of Pasadena ..."They Shall Not Have Dreamed in Vain"; Boy Scouts of America ..."Our Dream -- A Free Future"; California Exchange Clubs ..."Rocket to the Moon"; California State Polytechnic ..."A Free World Through Education".
DOI: 10.25549/examiner-c44-37170
title: "Hawaiian Pilot" freighter chartered for pleasure cruise, 1952

description: 9 images. "Hawaiian Pilot" freighter chartered for pleasure cruise, 16 May 1952. Frank T. Regan; Russell Combs; Captain Hans Matthieson; Mrs. P.H. Germaine; Mrs. Emma Lamoree; Mrs. Helen Forker; Mr. and Mrs. N.O. Dion; Mrs. Robert H. Carmichael; Robert H. Carmichael; Mrs. Elwood Johnson.; Supplementary slip reads: "Gershon. City Desk. Freighter chartered for pleasure cruise. Number 1: Frank T. Regan leans over chart to see how Second Mate Russell Combs of Matson freighter 'Hawaiian Pilot' points out what Diamond Head, a famous landfall in Hawaii, looks like on a chart to Mrs. Regan. Number 2: Captain Hans Matthieson, skipper of the 'Hawaiian Pilot', points rudder indicator as Mrs. P.H. Germaine holds wheel and husband looks on. It's not new to 'P.H.' -- he's been on the waterfront for years and is taking a 'bus man's holiday' in a cruise fostered by neighbors pooling idea. Number 3: Divorcees, they are going to 'catch up on their reading'. Photo shows Mrs. George Lamoree and Mrs. Helen Stanley Forker in deck chairs. Number 4: Mr. and Mrs. Robert H. Carmichael smile from the bridge railing. They belong to a colony deciding on a trip to Hawaii chartering freighter's passenger accommodations. Numbers 5-6: Frank T. 'Tim' Regan in lei on deck looks down on cabin cruiser of B.F. McDonald, manufacturer, wishing him 'Aloha'".
DOI: 10.25549/examiner-c44-37012
title: Bank robber and hold up, 1952

description: 4 images. Bank robber and hold up, 15 May 1952. Terry Spiegl (trailed and caused capture of robber); Officer J.B. Barton (who captured bandit); Omer Tessier (suspect).
DOI: 10.25549/examiner-c44-37063
title: Warner Brothers studio fire -- Burbank, 1952

description: 31 images. Warner Brothers studio fire -- Burbank, 1952. General scenes of fire.
DOI: 10.25549/examiner-c44-36421
title: Russell Dinner, 1952

description: 4 images. Russell Dinner, 12 May 1952. Senator Richard Russell; Y. Frank Freeman; Edwin W. Pauley; Pat Brown.
DOI: 10.25549/examiner-c44-41347
title: Grand Jury (Gangland investigations), 1952

description: 6 images. Grand Jury (Gangland investigations), 29 January 1952. Sam Farkas (former bodyguard of Mickey Cohen); Munson E. Moser (Officer); E. M. Goosen (Officer); Joseph Di Stefano; Ben P. Lobalbo.
DOI: 10.25549/examiner-c44-11730
title: Arthur Lake and family at Crestline, 1951

description: 12 images. Arthur Lake and family at Crestline, June 25, 1951. Arthur Lake; Patricia Lake; Arthur Lake; Marion Lake; Jerry Fitzgerald.
DOI: 10.25549/examiner-c44-8978
title: Accident, 1951

description: 4 images. Accident, May 10, 1951. Washington Place and Centinela Avenue; Mrs. Clara Reims; Elliott Reims -- 8 years.; More descriptive information with originals.
DOI: 10.15468/dl.8dvgua
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Acharagma roseanum subsp. galeanense (Haugg) D.R.Hunt"
 ]
}

 The dataset includes 14 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0016043-240506114902167/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/examiner-c44-37136
title: Flood ...Pioneer Boulevard in Artesia ...Pioneer Boulevard near Willis Street School, 1952

description: 9 images. Flood ...Pioneer Boulevard in Artesia ...Pioneer Boulevard near Willis Street School, 18 January 1952. Boatswain Mate Second Class George Vickers, carrying victims; Manuel Fernandes, carrying victims; Mrs. Lenna Kent, being carried to truck; Julia Kent, 15, flood victim, being carried to truck; Victoria Kent, 11, flood victim, being carried to truck; Charles Rorick, carrying child to truck; Michael Rorick, 8 months, his son; Mrs. Virginia Rorick, being carried to truck.; Caption slip reads: "Photographer: Olmo. Date: 01-18-52. Reporter: T.T. Assignment: Flood Damage. 34: Geo. A. Vickers, BM/2C -- Coast Guard carries Victoria Kent, 11, from house to truck. 33: Julia Kent, 15, carried to Coast Guard truck by Geo. A. Vickers, BM/2C. 35: l to r -- Bm/2c Geo. Vickers and Manuel Fernandes carry Mrs. Lenna Kent to truck. 37, 38, 39: Charles Rorick carries his child, Michael, 8 mo. to truck -- BM/2C George Vickers and Manuel Fernandes carry Mrs. Virginia Rorick to truck. 40: Traffic on Pioneer St. near Willis St. School. #41: General view of Poineer Blvd. on Artesia 186 block".
DOI: 10.25549/examiner-c44-10255
title: Cow Queen, 1951

description: 4 images. Cow Queen, May 26, 1951. Frank L. Pellissier (owner of Pellisseir Dairy Farm); Ruth Geske ("Miss Spring Fair of 1951); cow "Buttercup".
DOI: 10.25549/examiner-c44-9161
title: Dinner (American Air Force Association), 1951

description: 2 images. Dinner (American Air Force Association), May 12, 1951. General Albert C. Wedemeyer; Louis B. Mayer; General Carl Spaatz (retired).
DOI: 10.25549/examiner-c44-41240
title: Los Angeles City dump burning in violation in Chavez Ravine, 1951

description: 7 images. Los Angeles City dump burning in violation in Chavez Ravine, 9 February 1951. Shots from hill opposite 1518 North Boylston Street showing smoke from burning dump with City Hall tower and Federal Building in background; Verne Jones, 1321 West Poindexter Street, tends brush fire in accordance with orders; "No dumping or burning of rubbish" sign on property; Shots from hill near North Boylston Street and Garibaldi Drive showing billowing smoke from dump; Shots from atop bluff overlooking burning dump adjacent Paducah Street School showing City Hall tower in background. (Sleeve reads: 9593 was 9047).; Caption slip reads: "Photographer: Brunk. Date: 1951-02-09. Reporter: Rose. Assignment: City dump burning in violation of order in Chavez Ravine. 41-42-43-44: Long shot from hill opposite 1518 North Boylston Street, showing billowing smoke from burning dump with City Hall tower and Federal Building in background and homes in foreground. 47-48: Verne Jones, of 1321 West Poindexter Street, tends brush fire in accordance with orders. 45-46: 'No Dumping' or burning of rubbish sign on dump property. 25-26: Shot from hill near North Boylston Street and Garibaldi Dr. showing billowing smoke from dump. 27-28: Shots from atop bluff overlooking burning dump, adjacent to Paducah Street School and residential area, showing City Hall tower in background".
DOI: 10.25549/examiner-c44-41670
title: Tournament of Roses floats, 1952

description: 10 images. Tournament of Roses floats, 01 January 1952. Mexico ..."A Trip to Mexico".
DOI: 10.25549/examiner-c44-41377
title: Shooting of Sam Farkas, 1952

description: 6 images. Shooting of Sam Farkas, 28 January 1952. Sergeant J. J. Uhlick; Joe Di Stefano -- 36 years (suspect); Sam Farkas -- 34 years (victim); Officer M. E. Moser -- 25 years.
DOI: 10.25549/examiner-c44-76476
title: State Chamber of Commerce -- Biltmore, 1951

description: 3 images. State Chamber of Commerce -- Biltmore, 14 September 1951. Russell S. Bock; Adrien J. Falk; James Mussatti.; Caption slip reads: "Photographer: Paegel. Date: 1951-09-14. Assignment: State C. of C., Biltmore. 19/20/61: L to R: Russell S. Bock, Mgr., Tax Dept., Ernst & Ernst, L.A.; Adrien J. Falk, Pres., State Chamber, Pres. S & W Fine Foods, Inc., S.F.; James Mussatti, Mgr., State Chamber, S.F.".
DOI: 10.6084/m9.figshare.25832812
title: <b>Patterns and drivers of taxonomic and functional changes in oceanic island bird assemblages</b>

description: Dataset with 64 oceanic islands belonging to 11 archipelagos. Country, latitude, Island area, Isolation to surrounding landmass, Distance to mainland, Elevation range, Mean annual temperature, Temperature seasonality, Annual precipitation, Precipitation seasonality, Aridity index, Percentage of cultivated and managed vegetation, Human population density (est. 2015), Percentage of roadless areas, Harbour capacity, Airport capacity, Human colonization, Colonization pressure, Number of introduced mammal predators, Number of extinct bird species, Number of introduced bird species, Number of extant species, Species richness (original and present assemblage), Taxonomic dissimilarity, Functional dissimilarity, Changes in functional richness.
DOI: 10.25549/examiner-c44-76688
title: Arson investigation of Warner Brothers fire (Burbank studios), 1952

description: 11 images. Arson investigation of Warner Brothers fire (Burbank studios), 10 July 1952.
DOI: 10.25549/examiner-c44-57572
title: Examiner annual review, 1951-1953

description: 26 images. Examiner annual review, 1951-1953. General views of freeways.
DOI: 10.25549/examiner-c44-76436
title: Louella Parson exclusive (wedding), 1951

description: 2 images. Louella Parson exclusive (wedding), 23 September 1951.Lesley Starr; and Turner Blair Shelton. (Handout).
DOI: 10.25549/examiner-c44-40784
title: Note left at school by "The Phantom: warning school to close windows against possible "Burgeller", 1952

description: 2 images. Note left at school by "The Phantom: warning school to close windows against possible "Burgeller", 31 January 1952. Noble Avenue School in Sepulveda (not in picture).
DOI: 10.25549/examiner-c44-41981
title: Cab driver shot in West Los Angeles, 1952

description: 16 images. Cab driver shot in West Los Angeles, 5 June 1952. Robert Merritt (Officer Sergeant); Oliver Weathers (cab driver, victim, who died of shot wound); Nicholis Janise (Officer); H.M. Tinling (nurse); Mrs. Edith Weathers (wife of victim); James McDade (Yellow Cab Supervisor); Jack Fuller (Police Officer) (off duty); Jesse Burton (sister of Mrs. Weathers); Patty Weathers (daughter of victim); D.F. Mortell (dusting for fingerprints in cab that was abandoned on 11th Street between Hill Street & Olive Street); Lieutenant George Stoner.; Caption slip reads: "Photographer: Sandusky. Date: 1952-06-05. Assignment: Cab driver shot to death. D42: Left to right: Nurse H.M. Tinling assists Mrs. Edith Weathers and daughter Patty Weathers from room at Santa Monica where Mrs. Weathers collapsed after being informed that husband had died before she arrived. Behind Patty in doorway is Jesse Burton (either sister or sister-in-law to Mrs. Weathers) and Police officer Jack Fuller. D5: In cab leaving hospital, left to right: Jesse Burton; Mrs. Edith Weathers; Patty Weathers; Cab supervisor James McDade. D39: Left to right: Nurse H.M. Tinling; Mrs. Edith Weathers; Yellow Cab Supervisor James McDade. Mrs. Weathers has just been told her husband died while en route to hospital. D40: Police Officer (off-duty) Jack Fuller and Jesse Burton at Santa Monica Hospital. D41: Police Officer (off-duty) Jack Fuller and Patty Weathers, daughter of slain cab driver. Fuller is taking girl into room where mother, hysterical, has already been told Weathers died. 2 shots: Sergeant Robert Merritt and victim and ambulance men. 1 shot of Sergeant Robert Merritt and victim. Victim is Oliver Weathers, at scene of shooting".; Other caption slip reads: :Photographer: Glickman. Date: 1952-06-05. Assignment: Cab found, one used in murder, 11th Street between Hill Street & Olive Street. Assorted shots of cab and surrounding area; Man doing fingerprinting is D.F. Mortell, alongside of him in several pictures; tall man wearing hat is Lieutenant George Stoner. Miscellaneous shots of Mortell dusting for prints in cab. Bullet in hand -- bullet found in cab. G15: Sergeant Merritt looks over body as ambulance men work over victim. G14: Lieutenant Nicholis Janise counts change found at scene, cabbies hat in foreground".
DOI: 10.25549/examiner-c44-41747
title: Tournament of Roses floats, 1952

description: 9 images. Tournament of Roses floats, 01 January 1952. Union Rescue Mission ..."Living Water"; West Colorado Street Neighbors ..."Space Ship"; Young Republicans ..."Integrity in Government".
DOI: 10.25549/examiner-c44-41904
title: Probation convention, 1952

description: 2 images. Probation convention, 6 June 1952. Doctor A.R. Mangus (Principal speaker) (Research Sociologist).; Caption slip reads: "Photographer: Gaze. Date: 1952-06-06. Reporter: Stan Dunn. Assignment: Probation convention. #29-30: Doctor A.R. Mangus, research sociologist, Langley Porter Clinic, San Francisco, principal speaker today at California Probation and Parole Association conference, Santa Monica".
DOI: 10.25549/examiner-c44-41980
title: Cab driver shot in West Los Angeles, 1952

description: 16 images. Cab driver shot in West Los Angeles, 5 June 1952. Robert Merritt (Officer Sergeant); Oliver Weathers (cab driver, victim, who died of shot wound); Nicholis Janise (Officer); H.M. Tinling (nurse); Mrs. Edith Weathers (wife of victim); James McDade (Yellow Cab Supervisor); Jack Fuller (Police Officer) (off duty); Jesse Burton (sister of Mrs. Weathers); Patty Weathers (daughter of victim); D.F. Mortell (dusting for fingerprints in cab that was abandoned on 11th Street between Hill Street & Olive Street); Lieutenant George Stoner.; Caption slip reads: "Photographer: Sandusky. Date: 1952-06-05. Assignment: Cab driver shot to death. D42: Left to right: Nurse H.M. Tinling assists Mrs. Edith Weathers and daughter Patty Weathers from room at Santa Monica where Mrs. Weathers collapsed after being informed that husband had died before she arrived. Behind Patty in doorway is Jesse Burton (either sister or sister-in-law to Mrs. Weathers) and Police officer Jack Fuller. D5: In cab leaving hospital, left to right: Jesse Burton; Mrs. Edith Weathers; Patty Weathers; Cab supervisor James McDade. D39: Left to right: Nurse H.M. Tinling; Mrs. Edith Weathers; Yellow Cab Supervisor James McDade. Mrs. Weathers has just been told her husband died while en route to hospital. D40: Police Officer (off-duty) Jack Fuller and Jesse Burton at Santa Monica Hospital. D41: Police Officer (off-duty) Jack Fuller and Patty Weathers, daughter of slain cab driver. Fuller is taking girl into room where mother, hysterical, has already been told Weathers died. 2 shots: Sergeant Robert Merritt and victim and ambulance men. 1 shot of Sergeant Robert Merritt and victim. Victim is Oliver Weathers, at scene of shooting".; Other caption slip reads: :Photographer: Glickman. Date: 1952-06-05. Assignment: Cab found, one used in murder, 11th Street between Hill Street & Olive Street. Assorted shots of cab and surrounding area; Man doing fingerprinting is D.F. Mortell, alongside of him in several pictures; tall man wearing hat is Lieutenant George Stoner. Miscellaneous shots of Mortell dusting for prints in cab. Bullet in hand -- bullet found in cab. G15: Sergeant Merritt looks over body as ambulance men work over victim. G14: Lieutenant Nicholis Janise counts change found at scene, cabbies hat in foreground".
DOI: 10.25549/examiner-c44-41871
title: New store (Von's), 1952

description: 2 images. New store (Von's), 5 June 1952. New Von's Store at 6751 West Eightieth Street.
DOI: 10.25549/examiner-c44-56969
title: Traffic at 8300 Lankershim Boulevard, 1956

description: 4 images. Traffic at 8300 Lankershim Boulevard, 10 February 1956. Tin cans all over intersection.; Caption slip reads: "Photographer: Glickman. Date: 1956-02-10. Assignment: Traffic -- 8300 Lankershim Blvd. 4 shots: Tin can lids spewn over street intersection; truck in rear".
DOI: 10.25549/examiner-c44-57571
title: Examiner annual review, 1951-1953

description: 26 images. Examiner annual review, 1951-1953. General views of freeways.
DOI: 10.17632/68bhhsy2p4.1
title: FabT_+or-Tween_S.pyogenesM28_Transcriptomic analysis

description: We used RNAseq to identify how membrane fatty acid changes might impact and explain the virulence defect during host infection. WT and mFabT expression was compared in THY, and in THY-Tween (as C18:1Δ9 source), which to activates WT FabT repression. 
RNA isolation and Illumina RNA-seq sequencing:
GAS strains were cultured at 37°C in THY or THY-Tween, and cells were harvested during exponential growth (OD600 between 0.4 and 0.5). Independent triplicate cultures were prepared for each condition. For RNA preparation, 2 volumes of RNA protect (Qiagen) was added to cultures prior centrifugation (10 min 12,000 g) and total RNA was extracted after lysing bacteria by a 30 min 15 mg.ml-1 lysozyme, 300 U.ml-1 mutanolysin treatment at 20°C followed by two cycles of Fast-prep (power 6, 30 s) at 4 °C. RNA extraction (Macherey-Nagel RNA extraction kit; Germany) was done according to supplier instructions. RNA integrity was analyzed using an Agilent Bioanalyzer (Agilent Biotechnologies, Ca., USA). 23S and 16S rRNA were depleted from the samples using the MICROBExpress Bacterial mRNA enrichment kit (Invitrogen, France); depletion was controlled on Agilent Bioanalyzer (Agilent Biotechnologies). Libraries were prepared using an Illumina TS kit. Libraries were sequenced generating 10,000,000 to 20,000,000 75-bp-long reads per sample.
RNA-Seq data analysis:
The MGAS6180 strain sequence (NCBI), which is nearly identical to M28PF1, was used as a reference sequence to map sequencing reads using the STAR software (2.5.2b) BIOCONDA (Anaconda Inc). RNA-seq data were analyzed using the hclust function and a principal component analysis in R 3.5.1 (version 2018-07-02). For differential expression analysis, normalization and statistical analyses were performed using the SARTools package and DESeq2 p-values were calculated and adjusted for multiple testing using the false discovery rate controlling procedure. We used UpsetR to visualize set intersections in a matrix layout comprising the mFabT versus the WT strain grown in THY and in THY-Tween, and growth in THY-Tween versus THY for each strain.