cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.6wwpzgn09
title: FRET imaging and curvature data of freely moving C. elegans

description: The force - induced unfolding and refolding of proteins is speculated to
 be a key mechanism in the sensing and transduction of mechanical signals
 in the living cell. Yet, little evidence has been gathered for its
 existence in vivo . Prominently, s tretch - induced unfolding is
 postulated to be the activation mechanism of the t witchin/titin family of
 autoinhibited sarcomeric kinases linked to the mechanical stress response
 of muscle. To test the occurrence of mechanical kinase activation in
 living working muscle , we generated tra nsgenic C. elegans expressing tw
 itchin containing FRET moieties flanking the kinase domain and developed a
 quantitative technique for extracting FRET signals in freely moving C.
 elegans , using tracking and simultaneous imaging of animals in three
 channels (donor fluorescence, acceptor fluorescence, and transmitted
 light). Computer vision algorithms were used to extract fluorescence
 signals and muscle c ontraction states in each frame , in order to obtain
 fluorescence and body curvature measurements with spatial and temporal
 precision in vivo . The data reveal ed statistically significant periodic
 changes in FRET signals during muscle activity, consistent with a periodic
 change in the conforma tion of twitchin kinase. We conclude that stretch -
 unfolding of twitchin kinase occur s in the active muscle , whereby
 mechanical activity titrates the signalling pathway of th is cytoskeletal
 kinase . We anticipate that the methods we have developed here could be
 applied to obtaining in vivo evidence for force - induced conformational
 changes or elastic behavior of other proteins not only in C. elegans but
 in other animals in which there is optical tran sparency (e.g zebrafish).
description: All data was processed and saved using Matlab.
DOI: 10.5061/dryad.8cz8w9gqz
title: Trans-generational effect of protein restricted diet on adult body and wing size of Drosophila melanogaster

description: Protein restriction (PR) has established feasible trade-offs in Drosophila
 melanogaster to understand lifespan or aging in a nutritionally challenged
 environment. However, the phenotypes of body size, weight and wing length
 respond according to factors such as flies’ genotype, environmental
 exposure, and parental diet and hence their understanding is essential.
 Here, we demonstrate the effect of long-term PR diet on body size, weight,
 normal & dry wing length of flies subjected to PR50 and PR70 (50%
 and 70% protein content present in control food respectively) for 20
 generations from pre-adult stage. We found that PR fed flies have lower
 body weight, relative water content (in males), unaltered (PR50%) and
 higher (PR70%) relative fat content in males, smaller normal and dry body
 size as compared to control and generations 1 and 2. Interestingly, wing
 size and pupal size of PR flies are smaller and showed significant effects
 of diet and generation. Thus, these traits are sex and generation
 dependent along with an interaction of diet, which is capable of
 modulating these results variably. Taken together, the trans-generational
 effect of PR on fitness and fitness-related traits might be helpful to
 understand the underpinning mechanisms of evolution and aging in fruit
 flies D. melanogaster.
description: We measured the normal and dry body weight of freshly eclosed
 flies collected in every 2 h intervals. The eggs were collected from the
 DR stocks over a 2 h window and kept under LD12:12 h. Post eclosion, the
 virgin male and female flies were separated by anesthetizing with
 CO<sub>2</sub>. For weighing the normal body weight, flies
 were weighed post anesthetization using ether (to maintain the flies in
 the anesthetized state for longer duration), after which the flies were
 discarded. For the dry body weight assay, the virgin males and females
 were killed by freezing and were dried for 36 h at 70°C as per the
 protocol followed elsewhere. The normal and dry body weight assay was
 assessed by weighing a group of 10 males or 10 females per vial, and 5
 such vials of randomly chosen flies from the control and the DR stocks
 were weighed. The body weight of flies was measured using a weighing
 balance from UniBloc (Shimadzu) AUX220. The relative water content of the
 flies was calculated by dividing the water content (normal body weight-dry
 weight) by the normal body weight of the flies as reported elsewhere. The
 relative fat content was assessed by dividing the fat content (dry
 weight-fat free dry weight) by the dry weight of the flies. The flies’
 body size and wing length were measured under a microscope, wherein 30
 virgin males and females from the control and DR stocks were assayed and
 averaged and the values were used to plot the graph. The body size and the
 wing length of the anesthetized males and females were measured using a
 microscope from Olympus with a normal ruler (least count 0.5
 mm).
DOI: 10.5061/dryad.bcc2fqzcz
title: Data and statistical code from: Population differences in the effect of context on personality in an invasive lizard

description: Within populations, individuals often differ consistently in their average
 level of behavior (i.e. animal personality), as well as their response to
 environmental change (i.e. behavioral plasticity). Thus, changes in
 environmental conditions might be expected to mediate the structure of
 animal personality traits. However, it is currently not well understood
 how personality traits change in response to environmental conditions, and
 whether this effect is consistent across multiple populations within the
 same species. Accordingly, we investigated variation in personality traits
 across two ecological contexts in the invasive delicate skink
 (Lampropholis delicata). Specifically, lizards from three different
 populations were repeatedly measured for individual activity in group
 behavioral assays under differing levels of food availability. We found
 that environmental context had a clear effect on the structure of lizard
 personality, where activity rates were not repeatable in the absence of
 food, but were repeatable in the presence of food resources. The
 difference in repeatability of activity rates across contexts appeared to
 be largely driven by an increase in among-individual variance when tested
 in the presence of food resources. However, this was only true for one of
 the populations tested, with food context having no effect on the
 expression of personality traits in the other two populations. Our results
 highlight the important role of environmental context in mediating the
 structure of animal personality traits and suggest that this effect may
 vary among populations.
description: Usage notes are desrcibed in the associated README.txt file.
DOI: 10.5061/dryad.b8gtht7d5
title: Reduced host-plant specialization is associated with the rapid range expansion of a Mediterranean butterfly

description: Aim: Species ranges are highly dynamic, shifting in space and
 time as a result of complex ecological and evolutionary processes.
 Disentangling the relative contribution of both processes is challenging
 but of primary importance for forecasting species distributions under
 climate change. Here, we use the spectacular range expansion (ca. 1,000 km
 poleward shift within 10 years) of the butterfly Pieris
 mannii to unravel the factors underlying range dynamics,
 specifically the role of (i) niche evolution (changes in host-plant
 preference and acceptance) and (ii) ecological processes (climate
 change).  Location: Provence-Alpes-Côte d’Azur, France;
 North Rhine-Westphalia, Rhineland-Palatinate and Hesse, Germany.
 Taxon: Insect and angiosperms.  Methods: We
 employed a combination of (i) common garden experiments, based on
 replicated populations from the species’ historical and newly established
 range and host plant species representative for each distribution range,
 co-occurrence analyses and (ii) grid-based correlative species
 distribution modeling (SDM) using Maxent.   
 Results: We observed changes in oviposition preference, with
 females from the newly established populations showing reduced host-plant
 specialization and also an overall increased fecundity. These changes in
 behavior and life history may have enabled using a broader range of
 habitats and thus facilitated the recent range expansion. In contrast, our
 results indicate that the range expansion is unlikely to be directly
 caused by anthropogenic climate change, as the range was not constrained
 by climate in the first place. Main conclusions: We conclude that
 evolution of a broader dietary niche rather than climate change is
 associated with the rapid range expansion and discuss potential indirect
 consequences of climate change as trigger for the genetic differences
 found. Our study thus illustrates the importance of species interactions
 in shaping species distributions and range shifts, and draws attention to
 indirect effects of climate change. Embracing this complexity is likely
 key to a better understanding of range dynamics.
DOI: 10.57760/sciencedb.36388
title: Expression levels for all genes in leaves of Zhongmai 578 (ZM578) and Jimai 22 (JM22).
title: Expression levels for all genes in leaves of Zhongmai 578 (ZM578) and Jimai 22 (JM22).

description: Fresh leaves from ZM578 and JM22 were collected after 28 d of vernalization at 4 °C, and 7 d of growth under normal growing conditions (15–18 °C, 16 h light and 8 h darkness).All samples were quickly frozen in liquid nitrogen and stored at −80 °C for transcriptome sequencing assays with three biological replications. Transcriptomic data with a sequencing depth of 10 Gb for each sample were generated using the DNBSEQ T7 platform.
description: Fresh leaves from ZM578 and JM22 were collected after 28 d of vernalization at 4 °C, and 7 d of growth under normal growing conditions (15–18 °C, 16 h light and 8 h darkness).All samples were quickly frozen in liquid nitrogen and stored at −80 °C for transcriptome sequencing assays with three biological replications. Transcriptomic data with a sequencing depth of 10 Gb for each sample were generated using the DNBSEQ T7 platform.
DOI: 10.57760/sciencedb.36389
title: Expression levels of all genes in young spike of Zhongmai 578 (ZM578) and Jimai 22 (JM22).
title: Expression levels of all genes in young spike of Zhongmai 578 (ZM578) and Jimai 22 (JM22).

description: Young spikes were also harvested at the double ridge stage (Feekes 6). All samples were quickly frozen in liquid nitrogen and stored at −80 °C for transcriptome sequencing assays with three biological replications. Transcriptomic data with a sequencing depth of 10 Gb for each sample were generated using the DNBSEQ T7 platform.
description: Young spikes were also harvested at the double ridge stage (Feekes 6). All samples were quickly frozen in liquid nitrogen and stored at −80 °C for transcriptome sequencing assays with three biological replications. Transcriptomic data with a sequencing depth of 10 Gb for each sample were generated using the DNBSEQ T7 platform.
DOI: 10.57760/sciencedb.j00210.00107
title: Genotype information of Zhongmai 578/Jimai 22 recombinant inbred lines.
title: Genotype information of Zhongmai 578/Jimai 22 recombinant inbred lines.

description: Based on the results of the 50K SNP array for the recombinant inbred line (RIL) population derived from Zhongmai 578 and Jimai 22, monomorphic markers between the parents, markers with a missing value rate higher than 20.0%, and markers with a minor allele frequency (MAF) lower than 0.3 were excluded. After removing redundant markers using the BIN function in IciMapping v4.2 software ( http://www.isbreeding.net/), all the markers ultimately used for constructing the genetic linkage map were obtained
description: Based on the results of the 50K SNP array for the recombinant inbred line (RIL) population derived from Zhongmai 578 and Jimai 22, monomorphic markers between the parents, markers with a missing value rate higher than 20.0%, and markers with a minor allele frequency (MAF) lower than 0.3 were excluded. After removing redundant markers using the BIN function in IciMapping v4.2 software ( http://www.isbreeding.net/), all the markers ultimately used for constructing the genetic linkage map were obtained
DOI: 10.5061/dryad.v41ns1rx1
title: Spatial structure and dispersal dynamics in a house sparrow metapopulation

description: 1. The effects of spatial structure on metapopulation dynamics depend upon
 the interaction between local population dynamics and dispersal, and how
 this relationship is affected by the geographical isolation and spatial
 heterogeneity in habitat characteristics. 2. Our aim is to examine how
 emigration and immigration of house sparrows, Passer domesticus, in a
 Norwegian archipelagic metapopulation are affected by key factors
 predicted by classic metapopulation models to affect dispersal: spatial
 and temporal variation in population size, inter-island distance, local
 demography and habitat characteristics. 3. This metapopulation can be
 divided into two major habitat types: (1) islands closer to the mainland
 where sparrows breed in colonies on farms, and (2) islands without farms,
 situated farther away from the mainland where sparrows are exposed to
 harsher environmental conditions. 4. Dispersal was spatially structured
 within the metapopulation; there was proportionally and numerically less
 emigration and immigration involving farm islands, as compared to non-farm
 islands. Furthermore, emigration and immigration occurred mostly between
 nearby islands. Moreover, emigration in response to spatial differences in
 mean population size differed between the habitat types, but populations
 with large mean received more immigrants in both habitat types. The number
 of emigrants and immigrants was negatively related to long-term recruit
 production, which was not the case in non-farm islands. The proportion and
 number of emigrants was positively related to temporal increases in
 recruit production on farm islands, however, not on non-farm islands. 5.
 Our results demonstrate that spatial heterogeneity in environmental
 conditions influences how spatial variation in long-term mean population
 size, and temporal and spatial variation in recruit production, affects
 dispersal dynamics. The spatial structure of this metapopulation is
 therefore best described by a spatially explicit model in which the
 exchange of individuals within each habitat type is strongly affected by
 the degree of geographical isolation, population size and recruit
 production. However, these relationships differed between the two habitat
 types, with the non-farm islands showing similarities to a mainland-island
 model type of structure, whereas dispersal on the farm islands showed
 features more associated with source-sink or balanced dispersal models.
 Such differential dispersal dynamics between habitat types is expected to
 have important consequences for the ecological and evolutionary dynamics
 within this metapopulation.
description: The data contain island-specific annual numbers of emigrants,
 immigrants, recruits produced and adult population size. The first two
 sets cover emigration and immigration respectively. The third set comprise
 the same data aggregated on specific island-pairs.
DOI: 10.57760/sciencedb.36386
title: Phenotype information of Zhongmai 578/Jimai 22 recombinant inbred lines.
title: Phenotype information of Zhongmai 578/Jimai 22 recombinant inbred lines.

description: The best linear unbiased estimation (BLUE) values were calculated based on the phenotypic data of heading date (HD), spikelet number per spike (SNS), and fertile spikelet number (FSN) in the recombinant inbred line (RIL) population derived from Zhongmai 578 and Jimai 22 across multi-year and multi-location environments. The BLUE values for the RILs were calculated across all environments using the PROC MIXED function in SAS 9.4 (SAS Institute Inc., Cary, NC, USA).
description: The best linear unbiased estimation (BLUE) values were calculated based on the phenotypic data of heading date (HD), spikelet number per spike (SNS), and fertile spikelet number (FSN) in the recombinant inbred line (RIL) population derived from Zhongmai 578 and Jimai 22 across multi-year and multi-location environments. The BLUE values for the RILs were calculated across all environments using the PROC MIXED function in SAS 9.4 (SAS Institute Inc., Cary, NC, USA).
DOI: 10.5061/dryad.4j0zpc8c1
title: Data from: Novel nectar robbing negatively affects reproduction in Digitalis purpurea

description: With many plant-pollinator interactions undergoing change as species’
 distributions shift, we require a better understanding of how the addition
 of new interacting partners can affect plant reproduction. One such group
 of floral visitors, nectar robbers, can deplete plants of nectar rewards
 without contributing to pollination. The addition of nectar robbing to the
 floral visitor assemblage could therefore have costs to the plant´s
 reproductive output. We focus on a recent plant colonist, Digitalis
 purpurea, a plant that in its native range is rarely robbed, but
 experiences intense nectar robbing in areas it has been introduced to.
 Here, we test the costs to reproduction following experimental nectar
 robbing. To identify any changes in the behaviour of the principal
 pollinators in response to nectar robbing, we measured visitation rates,
 visit duration, proportion of flowers visited and rate of rejection of
 inflorescences. To find the effects of robbing on fitness, we used proxies
 for female and male components of reproductive output, by measuring the
 seeds produced per fruit and the pollen export respectively. Nectar
 robbing significantly reduced the rate of visitation and lengths of visits
 by bumblebees. Additionally, bumblebees visited a lower proportion of
 flowers on an inflorescence that had robbed flowers. We found that flowers
 in the robbed treatment produced significantly fewer seeds per fruit on
 average but did not export fewer pollen grains. Our finding that robbing
 leads to reduced seed production could be due to fewer and shorter visits
 to flowers leading to less effective pollination. We discuss the potential
 consequences of new pollinator environments, such as exposure to nectar
 robbing, for plant reproduction.
description: The Excel file includes the following tabs as follows; see
 legends for variable names in each tab within the file:
 Visits and Rejections: Visits by pollinators and number of times
 inflorescences were rejected. 
 Visit Duration: Length of time bumblebees spend, measured at the
 time between entering and exiting a flower.
 Proportions: Proportion of flowers in an inflorescence visited by
 bumblebees. Seed Production: Estimates of seed
 production for fruits. Pollen Export: Pollen exported
 from flowers in experimental plant. Pollen Within
 Flowers: Pollen grain production by anthers.
DOI: 10.5281/zenodo.18396885
title: IIS-EEG: Information impairment Susceptibility EEG Dataset

description: The Information impairment Susceptibility EEG Dataset (IIS-EEG) provides EEG recordings acquired during passive viewing of emotion-eliciting audiovisual video clips under three affective conditions: positive, neutral, and negative. Participants were selected from a larger questionnaire cohort using an extreme-group strategy based on susceptibility scores derived from the Personal Information Impairment Scale (PIIS). Only de-identified EEG recordings and essential metadata are released; questionnaire item-level responses are not distributed.
DOI: 10.5281/zenodo.18396886
title: IIS-EEG: Information impairment Susceptibility EEG Dataset

description: The Information impairment Susceptibility EEG Dataset (IIS-EEG) provides EEG recordings acquired during passive viewing of emotion-eliciting audiovisual video clips under three affective conditions: positive, neutral, and negative. Participants were selected from a larger questionnaire cohort using an extreme-group strategy based on susceptibility scores derived from the Personal Information Impairment Scale (PIIS). Only de-identified EEG recordings and essential metadata are released; questionnaire item-level responses are not distributed.
DOI: 10.5061/dryad.x69p8czjv
title: Dataset of nanoplastic transport in soil via bioturbation by Lumbricus terrestris

description: The dataset comprises data acquired in laboratory-based process-studies
 with microcosms on the transport of nanoplastics (polystyrene, 256 nm
 diameter) in an agricultural soil via bioturbation over the course of 4
 weeks. Transport of nanoplastics was monitored through the use of
 palladium (Pd)-doped nanoplastics (0.24 % w/w) by analyzing soil samples
 at specific depths (0-2 cm, 2-6 cm, 6-15 cm, 15-29 cm) after defined
 experimental times (7, 14, 21, 28 days). Microcosms consisted of
 30 cm height, 10 cm diameter soil columns which were set up with or
 without earthworms and with or without nanoplastics spiked to the
 uppermost 2 cm. Two experimental runs were executed with the first (Exp1)
 designed to focus on the average transport within the soil profile over
 time, and the second (Exp2) focussing on the smaller scale distribution of
 the nanoplastics in the soil (i.e. soil matrix versus drilosphere) while
 monitoring earthworm burrow development over time using X-ray computed
 tomography. In the first experiment, earthworms were measured for their
 nanoplastic content after the experiment. The dataset made available here
 comprises the details of the microcosm setup for Exp1 and Exp2, details on
 detected nanoplastic concentrations for the average soil profile
 (Exp1), drilosphere and soil matrix (Exp2), as well as detected
 concentrations in earthworm tissue and monitoring of earthworm weight
 (Exp1). A selection of X-ray CT images is also provided, chosen based on
 the considered highest relevance. X-ray CT scans of other measurement
 timepoints are available upon request.
description: The dataset comprises Setup
 details of the microcosms, including soil weight, spike concentrations and
 setup/sampling/measurement dates Detected
 Pd-concentrations in soil (Pd-doped nanoplastics), including soil matrix
 and drilosphere samples, and earthworm tissue with the corresponding
 nanoplastics concentrations; Method for detection and quantification:
 ICP-MS Grayscale images with minor pre-processing
 (reducing data volume by straightening and reducing canvas): X-ray
 computed tomography
description: Please read ReadMe file before usage and contact authors for
 further information or questions.