cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7419920.v1
title: A Família nos Agroecossistemas Amazônicos: o caso do Complexo Ambiental Sacaí, Baixo rio Branco, Roraima, Brasil

description: Abstract: The objective of this study was to characterize the family´s autopoiesis conservation movement in the agroecosystems of the Sacaí Environmental Complex, Baixo rio Branco, Caracaraí, Roraima, Brazil. The theoretical-methodological framework was based on the real understanding of the paradigm of systemic complexity. For this, it used a family of autopoietic organization analysis from the design of a multiple case study. Interviews with a previous script, census questionnaire, field diaries and photographs were used as evidence collection techniques. Forty-one families participated in this study. The paper revealed the importance of understanding the primary organizational structures in human ecological systems when considering conservation, which are not possible for standardization and replication in their entirety. From the results presented, it can be stated that the Sacaí Environmental Complex, as a result of the recursive process of autopoiesis, at the same time, assumes organization as a nuclear family, troncal extensive family and community extensive family. Thus, the Sacaí Environmental Complex, as an agroecossistemic organization, assumes the multiple possibility of life (re)construction from the family organization as a starting point for the conservation of autopoiesis.
DOI: 10.7284/116398
title: Winch data collected during research cruise NH1416 using a Markey DUSH-5 instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7419908
title: Phylogenomic and single nucleotide polymorphism analyses revealed the hybrid origin of Spondias bahiensis (family Anacardiaceae): de novo genome sequencing and comparative genomics

description: Abstract The genus Spondias (family Anacardiaceae) comprises 19 taxa, ten of which occur in Neotropical regions. Spondias bahiensis has been suggested to be a hybrid, although initial evidence does not support this hypothesis. The aim of this study was to test the hypothesis of the hybrid origin of S. bahiensis using high-throughput sequencing with single nucleotide polymorphism (SNP) analysis, characterization of intragenomic nuclear ribosomal DNA (nrDNA), and nuclear and chloroplast phylogenomic analyses. The SNP analysis revealed a high number of SNPs in the S. bahiensis genome, and with respect to nrDNA, S. bahiensis shared approximately half of the SNP alleles with S. tuberosa, but not with S. mombin. Combining the SNP analysis with nrDNA phylogeny confirmed the hybrid origin of S. bahiensis and put S. tuberosa as the female genitor. Considering the phylogeny of the genus Spondias and intraspecific SNPs in S. bahiensis, the putative male genitor is S. dulcis.
DOI: 10.6084/m9.figshare.7419911
title: Morpho-physiological characteristics by sweet potato cultivars as function of irrigation depth

description: Abstract The sweet potato is an alternative energy source, but its sustainability depends on efficient water use. The objective of this study was to evaluate sweet potato morpho-physiological characteristics, and water use efficiency (WUE). Irrigation depths of 50, 75, 100, and 125% of crop evapotranspiration (ETc) were applied. The morpho-physiological indicators, WUE, leaf area index (LAI), leaf water potential, leaf temperature, and leaf chlorophyll index (ICF), were evaluated. The WUE of the sweet potato cultivars increased until the 75% water depth of the ETc. The LAI of these cultivars increased with irrigation depth, with higher values at 100% of the ETc. The leaf water potential of the two sweet potato cultivars was lowest with the lower irrigation depth. Leaf temperature was closer to that of ambient temperatures in treatments with greater irrigation depth. The increase of the WUE with the greater accumulation of dry biomass is due to greater CO2 diffusion by stomata. The reduction in the growth of these plants is due to water stress limiting stomatal conductance, transpiration, leaf growth (LAI) and chlorophyll concentration, proportional to soil moisture conditions. The functional relationship between soil moisture and growth is essential to optimize irrigation management at different growth stages.
DOI: 10.6084/m9.figshare.7419917.v1
title: Lack of association between genetic polymorphisms in IGF1 and IGFBP3 with twin births in a Brazilian population (Cândido Godói, Rio Grande do Sul)

description: Abstract Insulin-like growth factor (IGF-1) is an important peptide hormone involved in the reproduction and fetal development of mammals, and it is suggested that it may influence the human twinning rate. This study aimed to test such possible association, investigating the genetic polymorphisms IGF1 (CA)n and IGFBP3 rs2854744 in the population from Candido Godoi (CG), a small city located in the South of Brazil that has a high prevalence of twin births. A case-control study was performed comprising a total of 39 cases (representing about 40% of the mothers of twins who were born in CG after 1995) and 214 controls (mothers of non-twin children), 97 of whom were living in CG while 117 were living in Porto Alegre. DNA was extracted from blood leucocytes and genotyping was performed. According to the statistical analyses, there was no significant difference in the frequencies of both studied genetic polymorphisms when comparing case group with control group. Thus, our results pointed to a lack of association between IGF1 (CA)n and IGFBP3 rs2854744 polymorphisms and twin births in CG, but further investigations in other populations with different characteristics must be performed to confirm the role of IGF-I in human twinning.
DOI: 10.6084/m9.figshare.7419905
title: Comparative cytogenetics in three Melipona species (Hymenoptera: Apidae) with two divergent heterochromatic patterns

description: Abstract The genus Melipona is subdivided into four subgenera based on morphological characteristics, and two groups based on cytogenetic patterns. The cytogenetic information on this genus is still scarce, therefore, the goal of this study was to characterize Melipona paraensis, Melipona puncticollis, and Melipona seminigra pernigra using the following techniques: C-banding, DAPI/CMA3 fluorochromes, and FISH with an 18S rDNA probe. Melipona paraensis (2n=18) and M. seminigra pernigra (2n=22) were classified as high heterochromatin content species (Group II). Their euchromatin is restricted to the ends of the chromosomes and is CMA3+; the 18S rDNA probe marked chromosome pair number 4. Melipona puncticollis (2n=18) is a low heterochromatin content species (Group I) with chromosome pair number 1 marked with CMA3 and 18S rDNA. Low heterochromatin content is a putative ancestral karyotype in this genus and high content is not a monophyletic trait (Melikerria presents species with both patterns). Differences concerning the karyotypic characteristics can be observed among Melipona species, revealing cytogenetic rearrangements that occurred during the evolution of this genus. Studies in other species will allow us to better understand the processes that shaped the chromatin evolution in Melipona.
DOI: 10.6084/m9.figshare.7419905.v1
title: Comparative cytogenetics in three Melipona species (Hymenoptera: Apidae) with two divergent heterochromatic patterns

description: Abstract The genus Melipona is subdivided into four subgenera based on morphological characteristics, and two groups based on cytogenetic patterns. The cytogenetic information on this genus is still scarce, therefore, the goal of this study was to characterize Melipona paraensis, Melipona puncticollis, and Melipona seminigra pernigra using the following techniques: C-banding, DAPI/CMA3 fluorochromes, and FISH with an 18S rDNA probe. Melipona paraensis (2n=18) and M. seminigra pernigra (2n=22) were classified as high heterochromatin content species (Group II). Their euchromatin is restricted to the ends of the chromosomes and is CMA3+; the 18S rDNA probe marked chromosome pair number 4. Melipona puncticollis (2n=18) is a low heterochromatin content species (Group I) with chromosome pair number 1 marked with CMA3 and 18S rDNA. Low heterochromatin content is a putative ancestral karyotype in this genus and high content is not a monophyletic trait (Melikerria presents species with both patterns). Differences concerning the karyotypic characteristics can be observed among Melipona species, revealing cytogenetic rearrangements that occurred during the evolution of this genus. Studies in other species will allow us to better understand the processes that shaped the chromatin evolution in Melipona.
DOI: 10.6084/m9.figshare.7419908.v1
title: Phylogenomic and single nucleotide polymorphism analyses revealed the hybrid origin of Spondias bahiensis (family Anacardiaceae): de novo genome sequencing and comparative genomics

description: Abstract The genus Spondias (family Anacardiaceae) comprises 19 taxa, ten of which occur in Neotropical regions. Spondias bahiensis has been suggested to be a hybrid, although initial evidence does not support this hypothesis. The aim of this study was to test the hypothesis of the hybrid origin of S. bahiensis using high-throughput sequencing with single nucleotide polymorphism (SNP) analysis, characterization of intragenomic nuclear ribosomal DNA (nrDNA), and nuclear and chloroplast phylogenomic analyses. The SNP analysis revealed a high number of SNPs in the S. bahiensis genome, and with respect to nrDNA, S. bahiensis shared approximately half of the SNP alleles with S. tuberosa, but not with S. mombin. Combining the SNP analysis with nrDNA phylogeny confirmed the hybrid origin of S. bahiensis and put S. tuberosa as the female genitor. Considering the phylogeny of the genus Spondias and intraspecific SNPs in S. bahiensis, the putative male genitor is S. dulcis.
DOI: 10.7284/116405
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise NH1417 using a Hawaii UHDAS instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7419902
title: Alterations in the lipid profiles and circulating liver enzymes in individuals infected by Schistosoma mansoni

description: Abstract INTRODUCTION Portal hypertension and periportal fibrosis commonly occur in severe Schistosoma mansoni infection. Changes in lipid profile and elevated levels of circulating liver enzymes have also been described in infected individuals. The present study sought to assess the alterations in laboratory parameters associated with liver disorder in individuals infected by S. mansoni who visited a private routine laboratory service. Levels of circulating liver enzymes (gamma-glutamyl transferase [γ-GT], aspartate transaminase [AST], alanine transaminase [ALT], and alkaline phosphatase [ALP]) and a lipid panel (total cholesterol [COL], high-density lipoprotein [HDL], low-density lipoprotein [LDL], very low-density lipoprotein [VLDL], and triglycerides [TRI]) were evaluated in both infected and non-infected individuals and relative risk was used to measure associations. METHODS Data were collected for analysis from a total of 1,078 cases identified in 379,600 individuals who submitted samples to the Datalab Laboratory (Salvador, Bahia) between 2004 and 2008. RESULTS S. mansoni infection led to increased circulating levels of γ-GT in both women and men, AST (women), and ALP (men). S. mansoni infection was a protective factor against increased levels of TRI, CHO, and VLDL for individuals aged 19 years or older. The results of our analysis indicate that alterations in lipid metabolism and circulating liver enzymes in asymptomatic S. mansoni-infected individuals might be attributed to eggs lodged in the hepatic sinusoids. CONCLUSIONS Parasitological testing for S. mansoni should be indicated in endemic areas when this pattern of alterations is detected.
DOI: 10.6084/m9.figshare.7419911.v1
title: Morpho-physiological characteristics by sweet potato cultivars as function of irrigation depth

description: Abstract The sweet potato is an alternative energy source, but its sustainability depends on efficient water use. The objective of this study was to evaluate sweet potato morpho-physiological characteristics, and water use efficiency (WUE). Irrigation depths of 50, 75, 100, and 125% of crop evapotranspiration (ETc) were applied. The morpho-physiological indicators, WUE, leaf area index (LAI), leaf water potential, leaf temperature, and leaf chlorophyll index (ICF), were evaluated. The WUE of the sweet potato cultivars increased until the 75% water depth of the ETc. The LAI of these cultivars increased with irrigation depth, with higher values at 100% of the ETc. The leaf water potential of the two sweet potato cultivars was lowest with the lower irrigation depth. Leaf temperature was closer to that of ambient temperatures in treatments with greater irrigation depth. The increase of the WUE with the greater accumulation of dry biomass is due to greater CO2 diffusion by stomata. The reduction in the growth of these plants is due to water stress limiting stomatal conductance, transpiration, leaf growth (LAI) and chlorophyll concentration, proportional to soil moisture conditions. The functional relationship between soil moisture and growth is essential to optimize irrigation management at different growth stages.
DOI: 10.6084/m9.figshare.7419896
title: Characteristics of Human Natal Stem Cells Cultured in Allogeneic Medium

description: Abstract Recently, human natal dental pulp stem cells (hNDP-SCs) have been characterized in vitro and it has been shown that they satisfy criteria defining human mesenchymal stromal cells (MSCs), as proposed by the International Society for Cellular Therapy. However, these results were reached in the presence of xenogeneic expansion medium, which has the potential to alter the cells’ functional capacity. To determine the validity of the previously reported hNDP-SCs characteristics for human cell therapy, we have cultured hNDP-SCs in allogeneic expansion medium. Two hNDP-SC lineages were isolated from vital natal teeth, donated by a healthy newborn female and cultured in 2% platelet rich plasma (PRP). Analysis of the phenotypic expressions, proliferation rates, viability, telomerase length and in vitro adipogenic, osteogenic and chondrogenic differentiation potentials of two hNDP-SCs lineages (Zn001 and Zn002) were performed. Both lineages displayed similar morphology, proliferation rates, adipogenic, chondrogenic and osteogenic differentiation potential. Telomere shortening by 41.0% and 13.49% occurred from 3rd till 14th passage for lineages Zn001 and Zn002 respectively. Viability of both lineages was higher than 90%. Flow cytometry demonstrated that both lineages were positive to the majority of tested markers, including markers, which were negatively, expressed when hNDP-SCs were cultured previously in xenogeneic medium. Using immune-cytochemistry the cells were shown to express beta III-tubulin, nestin, neurofilaments and Nanog. PRP used as allogeneic medium is suitable for cultivation of hNDP-SCs.
DOI: 10.6084/m9.figshare.7419902.v1
title: Alterations in the lipid profiles and circulating liver enzymes in individuals infected by Schistosoma mansoni

description: Abstract INTRODUCTION Portal hypertension and periportal fibrosis commonly occur in severe Schistosoma mansoni infection. Changes in lipid profile and elevated levels of circulating liver enzymes have also been described in infected individuals. The present study sought to assess the alterations in laboratory parameters associated with liver disorder in individuals infected by S. mansoni who visited a private routine laboratory service. Levels of circulating liver enzymes (gamma-glutamyl transferase [γ-GT], aspartate transaminase [AST], alanine transaminase [ALT], and alkaline phosphatase [ALP]) and a lipid panel (total cholesterol [COL], high-density lipoprotein [HDL], low-density lipoprotein [LDL], very low-density lipoprotein [VLDL], and triglycerides [TRI]) were evaluated in both infected and non-infected individuals and relative risk was used to measure associations. METHODS Data were collected for analysis from a total of 1,078 cases identified in 379,600 individuals who submitted samples to the Datalab Laboratory (Salvador, Bahia) between 2004 and 2008. RESULTS S. mansoni infection led to increased circulating levels of γ-GT in both women and men, AST (women), and ALP (men). S. mansoni infection was a protective factor against increased levels of TRI, CHO, and VLDL for individuals aged 19 years or older. The results of our analysis indicate that alterations in lipid metabolism and circulating liver enzymes in asymptomatic S. mansoni-infected individuals might be attributed to eggs lodged in the hepatic sinusoids. CONCLUSIONS Parasitological testing for S. mansoni should be indicated in endemic areas when this pattern of alterations is detected.
DOI: 10.7284/116414
title: CTD (Conductivity, Temperature, Depth) data collected during research cruise NH1417 using a Sea-Bird SBE-911+ instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7419890
title: Novel variants of human herpesvirus 2 from Brazilian HIV-1 coinfected subjects

description: BACKGROUND Human herpesvirus 2 (HHV-2) have DNA genome with a limited genetic variability and have been classified into two clades. OBJECTIVES To identify and characterise six HHV-2 isolates derived from Brazilian women. METHODS HHV-2 isolates were performed polymerase chain reaction (PCR) and sequencing of 2250 pb of the glycoprotein B (gB) coding regions. FINDINGS Four HHV-2 isolates were classified into clade B, while the remaining two, derived from HIV-1 co-infected women, showed a notable genetic divergence (&gt; 1%). MAIN CONCLUSION The results reveal novel HHV-2 variants. The impact of these novel variants on HHV-2 pathogenesis and HIV/HHV-2 coinfection need to be investigated.
DOI: 10.6084/m9.figshare.7419896.v1
title: Characteristics of Human Natal Stem Cells Cultured in Allogeneic Medium

description: Abstract Recently, human natal dental pulp stem cells (hNDP-SCs) have been characterized in vitro and it has been shown that they satisfy criteria defining human mesenchymal stromal cells (MSCs), as proposed by the International Society for Cellular Therapy. However, these results were reached in the presence of xenogeneic expansion medium, which has the potential to alter the cells’ functional capacity. To determine the validity of the previously reported hNDP-SCs characteristics for human cell therapy, we have cultured hNDP-SCs in allogeneic expansion medium. Two hNDP-SC lineages were isolated from vital natal teeth, donated by a healthy newborn female and cultured in 2% platelet rich plasma (PRP). Analysis of the phenotypic expressions, proliferation rates, viability, telomerase length and in vitro adipogenic, osteogenic and chondrogenic differentiation potentials of two hNDP-SCs lineages (Zn001 and Zn002) were performed. Both lineages displayed similar morphology, proliferation rates, adipogenic, chondrogenic and osteogenic differentiation potential. Telomere shortening by 41.0% and 13.49% occurred from 3rd till 14th passage for lineages Zn001 and Zn002 respectively. Viability of both lineages was higher than 90%. Flow cytometry demonstrated that both lineages were positive to the majority of tested markers, including markers, which were negatively, expressed when hNDP-SCs were cultured previously in xenogeneic medium. Using immune-cytochemistry the cells were shown to express beta III-tubulin, nestin, neurofilaments and Nanog. PRP used as allogeneic medium is suitable for cultivation of hNDP-SCs.
DOI: 10.6084/m9.figshare.7419887
title: G26P[19] rotavirus A strain causing acute gastroenteritis in the American continent

description: In Brazil, the rotavirus A genotype G26 was first identified in suckling piglets, while the P[19] genotype has not been identified in any animal species so far. This report details the genetic characterisation of a G26P[19] RVA strain detected from an eight year-old child, vaccinated with Rotarix®, hospitalised with acute diarrhoeal disease in Rio de Janeiro in 2015. Most likely, the genome constellation (I5-R1-C1-M1-A8-N1-T1-E1-H1) observed in the G26P[19] Brazilian strain was a result of interspecies transmission events between humans and pigs. In addition, a rearrangement in the NSP5 gene was observed downstream of the 3’ non-coding region.
DOI: 10.6084/m9.figshare.7419887.v1
title: G26P[19] rotavirus A strain causing acute gastroenteritis in the American continent

description: In Brazil, the rotavirus A genotype G26 was first identified in suckling piglets, while the P[19] genotype has not been identified in any animal species so far. This report details the genetic characterisation of a G26P[19] RVA strain detected from an eight year-old child, vaccinated with Rotarix®, hospitalised with acute diarrhoeal disease in Rio de Janeiro in 2015. Most likely, the genome constellation (I5-R1-C1-M1-A8-N1-T1-E1-H1) observed in the G26P[19] Brazilian strain was a result of interspecies transmission events between humans and pigs. In addition, a rearrangement in the NSP5 gene was observed downstream of the 3’ non-coding region.
DOI: 10.6084/m9.figshare.7419890.v1
title: Novel variants of human herpesvirus 2 from Brazilian HIV-1 coinfected subjects

description: BACKGROUND Human herpesvirus 2 (HHV-2) have DNA genome with a limited genetic variability and have been classified into two clades. OBJECTIVES To identify and characterise six HHV-2 isolates derived from Brazilian women. METHODS HHV-2 isolates were performed polymerase chain reaction (PCR) and sequencing of 2250 pb of the glycoprotein B (gB) coding regions. FINDINGS Four HHV-2 isolates were classified into clade B, while the remaining two, derived from HIV-1 co-infected women, showed a notable genetic divergence (&gt; 1%). MAIN CONCLUSION The results reveal novel HHV-2 variants. The impact of these novel variants on HHV-2 pathogenesis and HIV/HHV-2 coinfection need to be investigated.
DOI: 10.7284/116408
title: GNSS (Global Navigation Satellite System) data collected during research cruise NH1417 using a Furuno GP-150 instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7419881
title: Handgrip strength as a predictor of sensorimotor recovery evaluated by the Fugl-Meyer scale

description: ABSTRACT Cerebrovascular accidents can leave neurological, motor and sensory sequelae. To assess and monitor the patient’s prognosis, several functional measuring instruments are used, such as the Fugl-Meyer scale, which, although widely used to estimate the sensorimotor recovery, is a long evaluation that requires training. Therefore, the objective of this study is to analyze if the handgrip strength (HGS), the Timed up and Go test (TUG) and the Functional Independence Measure (FIM) can predict the results of the Fugl-Meyer scale, in order to optimize time during sensorimotor recovery assessment, both to monitor treatment responses and for scientific research. Thus, the HGS of 35 chronic hemiparetic patients was evaluated and then applied to Fugl-Meyer Scale, which evaluates motor recovery, the FIM, which evaluates motor activities and the TUG, which is an indicative of functional mobility. Statistical analysis was performed using multiple regression (r2). The HGS was predictive of motor recovery (r2=0.46; p=0.001), while mobility (r2=0.255; p=0.007) and functional independence (r2=0.054; p=0.2) were not capable of predicting the results of the Fugl-Meyer scale. After analysis, it was concluded that HGS is a moderate predictor of motor recovery after cerebrovascular accident, while mobility and functional independence are not.