cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14290866.v1
title: Exploring the shared genes of hypertension, diabetes and hyperlipidemia based on microarray

description: Given their relationship with metabolic syndrome and systematic inflammatory diseases, the pathogenesis of hypertension, hyperglycemia, and hyperlipidemia is closely related. To explore the common genes among these three conditions, spontaneous hypertensive rats (SHR), spontaneous diabetic Goto-Kakizaki rats (GK) and hyperlipidemia rats (HMR) were reared for experiments. Gene array was used to identify the genes of SHR, GK and HMR compared with normal Wistar rats using TBtools software. First, real-time PCR was applied to verify these genes, and Cytoscape software was used to construct networks based on the National Center for Biotechnology Information (NCBI) database. Second, Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis was performed to classify the genes. Visualization and Integrated Discovery (DAVID) database and Gene Ontology database were used to explore the biological function. Finally, Onto-tools Pathway Express was used to analyze the pathways of shared genes. Importantly, upregulated common genes, such as Bad, Orm1, Arntl and Zbtb7a, were used to construct a network of 150 genes, while downregulated genes, such as Mif and Gpx1, formed a network of 29 genes. Interestingly, the networks were involved in various pathways, such as insulin signal pathway, endometrial cancer pathway, circadian rhythm pathway, and pancreatic cancer pathway. We discovered common genes of SHR, GK and HMR compared with normal Wistar rats, and the association of these genes together with biological function were preliminarily revealed.
DOI: 10.6084/m9.figshare.14290880
title: Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats

description: T lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points. Isoniazid significantly increased the percentage of CD4+T lymphocytes and the CD4+/CD8+T lymphocyte cell ratio (P &lt; 0.05). It transiently (&lt;1 month) enhanced the functions of rat macrophages significantly (P &lt; 0.05). In summary, isoniazid could increase the percentage of CD4+T lymphocytes, CD4+/CD8+T lymphocyte cell ratio, and enhance macrophage function in healthy rats.
DOI: 10.6084/m9.figshare.14815167.v1
title: Identification of significant genes and therapeutic agents for breast cancer by integrated genomics

description: Breast cancer is the most commonly diagnosed malignancy in women; thus, more cancer prevention research is urgently needed. The aim of this study was to predict potential therapeutic agents for breast cancer and determine their molecular mechanisms using integrated bioinformatics. Summary data from a large genome-wide association study of breast cancer was derived from the UK Biobank. The gene expression profile of breast cancer was from the Oncomine database. We performed a network-wide association study and gene set enrichment analysis to identify the significant genes in breast cancer. Then, we performed Gene Ontology analysis using the STRING database and conducted Kyoto Encyclopedia of Genes and Genomes pathway analysis using Cytoscape software. We verified our results using the Gene Expression Profile Interactive Analysis, PROgeneV2, and Human Protein Atlas databases. Connectivity map analysis was used to identify small-molecule compounds that are potential therapeutic agents for breast cancer. We identified 10 significant genes in breast cancer based on the gene expression profile and genome-wide association study. A total of 65 small-molecule compounds were found to be potential therapeutic agents for breast cancer.
DOI: 10.6084/m9.figshare.14290880.v1
title: Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats

description: T lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points. Isoniazid significantly increased the percentage of CD4+T lymphocytes and the CD4+/CD8+T lymphocyte cell ratio (P &lt; 0.05). It transiently (&lt;1 month) enhanced the functions of rat macrophages significantly (P &lt; 0.05). In summary, isoniazid could increase the percentage of CD4+T lymphocytes, CD4+/CD8+T lymphocyte cell ratio, and enhance macrophage function in healthy rats.
DOI: 10.6084/m9.figshare.16912508.v2
title: VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

description: Chronic skin ulcers are a primary global health problem. Velvet antler polypeptide (VAP) regulates endothelial cell migration and angiogenic sprout. Adipose-derived stem cells (ADSCs) are reported to make pivotal impacts upon wound healing. This study aimed to explore the role of VAP combined with ADSCs in wound healing of chronic skin ulcers. The effect of VAP on phenotypes of ADSCs, and VAP (PLGA microspheres) combining with ADSCs on wound healing of chronic skin ulcers <i>in vivo</i> was evaluated. VAP generally promoted the proliferation, migration and invasion of ADSCs, and ADSC-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) through PI3K/Akt/HIF-1α pathway. VAP-PLGA (PLGA microspheres) enhanced the promoting effect of ADSCs on wound healing, pathological changes, and angiogenesis in chronic skin ulcers <i>in vivo</i>. VAP-PLGA intensified the effect of ADSCs on up-regulating the levels of p-PI3K/PI3K, p-Akt/Akt, HIF-1α, vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), C-X-C motif chemokine receptor 4 (CXCR4), angiopoietin-4 (Ang-4), VEGF receptor (VEGFR), and transforming growth factor-β1 (TGF-β1), and down-regulating the levels of interleukin-1 β (IL-1β), IL-18 and IL-6 in wound tissues in chronic skin ulcers <i>in vivo</i>. Collectively, VAP promoted the growth, migration, invasion, and angiogenesis of ADSCs through activating PI3K/Akt/HIF-1α pathway, and VAP-PLGA enhanced the function of ADSCs in promoting wound healing <i>in vivo</i>, which was associated with angiogenesis, inflammation inhibition, and dermal collagen synthesis.
DOI: 10.6084/m9.figshare.21106625.v1
title: A bio-inspired 3D metamaterials with chirality and anti-chirality topology fabricated by 4D printing

description: Artificial architected metamaterials equipped with unique mechanical and physical properties that are naturally inaccessible can be obtained by rational design. In this work, the innovative three-dimensional (3D) chiral and anti-chiral metamaterials are developed referring to the face-rotating polyhedral (FRP) structure present in the virus. Through assembling planar triangular units into the regular octahedron cells, several typical forms of chiral and anti-chiral metamaterials can be obtained by different assembly methods. By changing the topology parameters, the Poisson’s ratio can be adjusted between [0, 2.8]. The metamaterials are fabricated by 3D printing utilizing shape memory polymer, and the mechanical properties are analyzed via Finite Element Analysis (FEA) and experiments, including Young’s modulus, Poisson’s ratio, and tension-twist coupling behavior. In addition, target metamaterial with specific local deformation behavior is obtained by programmatic calculations and distributions to meet special requirements or achieve unique applications. The shape memory property endows the mechanical metamaterials with more potential applications.
DOI: 10.6084/m9.figshare.7479041.v1
title: Additional file 2: of The degree of mitochondrial DNA methylation in tumor models of glioblastoma and osteosarcoma

description: The differences in mtDNA methylation amongst the 143B143B, 143BGBM and 143BNSC mitochondrial genomes. (XLSX 59 kb)
DOI: 10.24403/jp.1323269
title: Beitrag zur Sippengliederung der Gattung Sigesbeckia L. (Asteraceae — Heliantheae — Melampodiinae) in Mexiko und auf den Karibischen Inseln / Dorothea L. Schulz
DOI: 10.6084/m9.figshare.20057439
title: Additional file 1 of Role of circulating polyunsaturated fatty acids on cardiovascular diseases risk: analysis using Mendelian randomization and fatty acid genetic association data from over 114,000 UK Biobank participants

description: Additional file 1: Supplementary Tables S1–S11.
DOI: 10.24403/jp.1323283
title: Sedlag, U. und Weinert, E.: Biogeographie, Artbildung, Evolution. - Jena : VEB Gustav Fischer Verlag, 1987 / [rezensiert von] H. Manitz
DOI: 10.6084/m9.figshare.19533960.v1
title: Additional file 1 of Comparative and phylogenetic analyses of six Kenya Polystachya (Orchidaceae) species based on the complete chloroplast genome sequences

description: Additional file 1: Table S1. Taxonomic and GenBank accession information forsamples used for phylogenetic analyses (85). Table S2 and S3. Types of genes annotation and the intron-containing genes within the chloroplastgenomes of six Polystachya species. Table S4. Codonusage within the chloroplast genomes of six Polystachyaspecies. Table S5. Typesand amounts of SSRs within the chloroplast genomes of six Polystachya species. Table S6. Locationof repeat sequences within the chloroplast genomes of six Polystachya species. Table S7. Comparisonof site models for the 68 shared CDSs in the chloroplast genomes of six Polystachyaspecies and results of LRT. Table S8. Positiveselection sites based on BEB analysis in the M8 model detected in thechloroplast genomes of six Polystachya species. Table S9. Theoverall view of all gene alignment in the complete chloroplast genomes of six Polystachyaspecies. Table S10. Phylogenetictree constructed using ML and BI methods, based on the first and second codonpositions of 79 CDSs of whole cp genomes from 85 taxa.
DOI: 10.6084/m9.figshare.7479047
title: Additional file 4: of The degree of mitochondrial DNA methylation in tumor models of glioblastoma and osteosarcoma

description: Primer pairs for long and real-time PCR and pyrosequencing. (XLSX 10 kb)
DOI: 10.6084/m9.figshare.14290809
title: Application of biosynthesized ZnO nanoparticles on an electrochemical H 2 O 2 biosensor

description: ABSTRACT ZnO nanoparticles (NPs) were synthesized via a green biochemical method using Corymbia citriodora leaf extract as a reducing and stabilizing agent. The biosynthesized ZnO NPs were characterized by SEM and XRD. An electrochemical H2O2 biosensor was fabricated by modification of a glassy carbon electrode using our proposed ZnO NPs. The electrochemical sensor showed excellent detection performance towards trace amounts of H2O2, demonstrating that it could potentially be used in clinical applications.
DOI: 10.6084/m9.figshare.14290809.v1
title: Application of biosynthesized ZnO nanoparticles on an electrochemical H 2 O 2 biosensor

description: ABSTRACT ZnO nanoparticles (NPs) were synthesized via a green biochemical method using Corymbia citriodora leaf extract as a reducing and stabilizing agent. The biosynthesized ZnO NPs were characterized by SEM and XRD. An electrochemical H2O2 biosensor was fabricated by modification of a glassy carbon electrode using our proposed ZnO NPs. The electrochemical sensor showed excellent detection performance towards trace amounts of H2O2, demonstrating that it could potentially be used in clinical applications.
DOI: 10.6084/m9.figshare.14290916.v1
title: Safety and tolerability of a single dose T0001 in Chinese healthy adult volunteers: a first-in-human ascending dose study

description: T0001 is the first mutant of etanercept with a higher affinity to tumor necrosis factor α (TNF-α) than etanercept. In order to investigate the safety and tolerability of T0001, a study was carried out in healthy Chinese subjects. A first-in-human, dose escalation study was conducted in healthy Chinese subjects. Fifty-six subjects were divided into six dose cohorts (10 mg, 20 mg, 35 mg, 50 mg, 65 mg and 75 mg) to receive a single subcutaneous injection of T0001. Safety and tolerability assessment were based on the records of vital signs, physical examinations, clinical laboratory tests, 12-lead electrocardiograms and adverse events (AEs). All subjects were in good compliance and none withdraw due to AEs. No serious AEs occurred. A total of twenty-three AEs in sixteen subjects were recorded, and eighteen of these AEs were believed to be related to T0001. The most frequently reported AEs were injection site reactions and white blood cell count increase. All these AEs were of mild to moderate intensity and most of them recovered spontaneously within 14 days. In this study, no dose-limiting toxicity was observed, and the maximum tolerated dose was identified as 75 mg. T0001 was considered safe and generally well tolerated at doses up to 75 mg in healthy Chinese volunteers.
DOI: 10.6084/m9.figshare.14290916
title: Safety and tolerability of a single dose T0001 in Chinese healthy adult volunteers: a first-in-human ascending dose study

description: T0001 is the first mutant of etanercept with a higher affinity to tumor necrosis factor α (TNF-α) than etanercept. In order to investigate the safety and tolerability of T0001, a study was carried out in healthy Chinese subjects. A first-in-human, dose escalation study was conducted in healthy Chinese subjects. Fifty-six subjects were divided into six dose cohorts (10 mg, 20 mg, 35 mg, 50 mg, 65 mg and 75 mg) to receive a single subcutaneous injection of T0001. Safety and tolerability assessment were based on the records of vital signs, physical examinations, clinical laboratory tests, 12-lead electrocardiograms and adverse events (AEs). All subjects were in good compliance and none withdraw due to AEs. No serious AEs occurred. A total of twenty-three AEs in sixteen subjects were recorded, and eighteen of these AEs were believed to be related to T0001. The most frequently reported AEs were injection site reactions and white blood cell count increase. All these AEs were of mild to moderate intensity and most of them recovered spontaneously within 14 days. In this study, no dose-limiting toxicity was observed, and the maximum tolerated dose was identified as 75 mg. T0001 was considered safe and generally well tolerated at doses up to 75 mg in healthy Chinese volunteers.
DOI: 10.6084/m9.figshare.14812492
title: Additional file 19 of Conversion between 100-million-year-old duplicated genes contributes to rice subspecies divergence

description: Additional file 19: Table S12. Expression level of converted and nonconverted gene pairs in GJ, XI-MH63, and XI-ZS97.
DOI: 10.6084/m9.figshare.19533870
title: Additional file 1 of Genome-wide identification and expression of SAUR gene family in peanut (Arachis hypogaea L.) and functional identification of AhSAUR3 in drought tolerance

description: Additional file 1.
DOI: 10.6084/m9.figshare.5902345.v1
title: A rapid method to identify <i>Salmonella enterica</i> serovar Gallinarum biovar Pullorum using a specific target gene <i>ipaJ</i>

description: <i>Salmonella enterica</i> serovar Gallinarum biovar Pullorum (<i>S.</i> Pullorum) is the pathogen of pullorum disease, which leads to severe economic losses in many developing countries. Traditional methods to identify <i>S. enterica</i> have relied on biochemical reactions and serotyping, which are time-consuming with accurate identification if properly carried out. In this study, we developed a rapid polymerase chain reaction (PCR) method targeting the specific gene <i>ipaJ</i> to detect <i>S.</i> Pullorum. Among the 650 <i>S.</i> Pullorum strains isolated from 1962 to 2016 all over China, 644 strains were identified to harbour <i>ipaJ</i> gene in the plasmid pSPI12, accounting for a detection rate of 99.08%. Six strains were <i>ipaJ</i> negative because pSPI12 was not found in these strains according to whole genome sequencing results. There was no cross-reaction with other <i>Salmonella</i> serotypes, including <i>Salmonella enterica</i> serovar Gallinarum biovar Gallinarum (<i>S</i>. Gallinarum), which show a close genetic relationship with <i>S.</i> Pullorum. This shows that the PCR method could distinguish <i>S.</i> Gallinarum from <i>S.</i> Pullorum in one-step PCR without complicated biochemical identification. The limit of detection of this PCR method was as low as 90 fg/μl or 10<sup>2</sup> CFU, which shows a high sensitivity. Moreover, this method was applied to identify <i>Salmonella</i> isolated from the chicken farm and the results were consistent with what we obtained from biochemical reactions and serotyping. Together, all the results demonstrated that this one-step PCR method is simple and feasible to efficiently identify <i>S.</i> Pullorum.
DOI: 10.6084/m9.figshare.14291418.v1
title: LncRNA NORAD engages in psoriasis by binding to miR-26a to regulate keratinocyte proliferation

description: Psoriasis is a chronic, inflammatory skin disease. It was reported that lncRNA Non-coding RNA-activated by DNA damage (NORAD) has potential regulatory effects on skin diseases. Our previous studies found that lncRNA NORAD was highly expressed and its potential target miR-26a was down-regulated in psoriasis model mice. Here, we aimed to investigate the role of NORAD in the development of psoriasis. IL-22/LPS (interleukin-22/lipopolysaccharide)-stimulated HaCaT (human immortalized keratinocytes) cell model and imiquimod-induced mouse model were established. Keratin 6 (K6), Keratin 16 (K16), Keratin 17 (K17), and Cell division cycle 6 (CDC6) levels were detected by western blot. Cell activity was detected by CCK-8, MTT, and EdU assays. Quantitative real-time PCR was performed to examine the levels of NORAD, miR-26a, CDC6, K6, K16, and K17. Haematoxylin-eosin staining was applied to observe the degree of skin thickening and hyperplasia. Fluorescence <i>in situ</i> hybridization detects the location of NORAD. RNA immunoprecipitation, RNA pull-down, and Luciferase test were performed to detect the interaction between NORAD and miR-26a. In IL-22/LPS-stimulated HaCaT cells, NORAD, CDC6, and keratinocyte proliferation-related proteins (K6, K16, and K17) were up-regulated and miR-26a was down-regulated. Cell survival and proliferation were also increased. However, the results were reversed after interference with NORAD. Also, <i>in vitro</i> experiments revealed that NORAD negatively regulated miR-26a. In IL-22/LPS-stimulated HaCaT cells and skin of imiquimod-induced mice, we found that lower NORAD resulted in an increase of miR-26a and a decrease of CDC6, further decreased levels of keratinocyte proliferation-related proteins (K6, K16, and K17).
DOI: 10.6084/m9.figshare.7479113.v1
title: Additional file 3: of Complex regulation of microRNAs in roots of competitively-grown isogenic Nicotiana attenuata plants with different capacities to interact with arbuscular mycorrhizal fungi

description: Table S3. Differentially expressed miRNAs for EV and irCCamK with and without AMF. (XLSX 41 kb)
DOI: 10.6084/m9.figshare.14812471
title: Additional file 14 of Conversion between 100-million-year-old duplicated genes contributes to rice subspecies divergence

description: Additional file 14: Table S8. GO analysis of converted genes and nonconverted genes in GJ, XI-MH63, and XI-ZS97.
DOI: 10.6084/m9.figshare.13295831.v16
title: Autism Spectrum Disorder and Asperger Syndrome Question Answering Dataset 1.0

description: <em><strong>RUS</strong></em> <strong>Последнее обновление: 23/05/2022</strong> Набор данных предназначен для разработки русскоязычных диалоговых систем (чат-ботов, вопросно-ответных систем и т. д.) о расстройствах аутистического спектра. Источник текстов: https://aspergers.ru Проект реализуется победителем конкурса «Практики личной филантропии и альтруизма» Благотворительного фонда Владимира Потанина. <strong>Состав набора данных:</strong> 1. original.json: оригинальная версия датасета 2. multiple.json: версия датасета с несколькими вариантами ответа 3. short.json: версия датасета с укороченными ответами 4. half_sized.json: версия датасета содержит 50% собранных данных 5. no_impossible.json: версия содержит только релевантные вопросы 7. age_dataset.tsv: набор данных для определения возраста пользователя (можно использовать для кастомизации моделей) <em><strong>ENG</strong></em> A dataset for question-answering used for building an informational Russian language chatbot for the inclusion of people with autism spectrum disorder and Asperger syndrome in particular, based on data from the following website: https://aspergers.ru. <strong>The dataset has several versions:</strong> 1. Original version 2. Half-sized version (50% of the original data) 3. No impossible version (a version without irrelevant/impossible questions) 4. Short version (a version with shorterned answers) 5. Multiple version (a version with several answers, all the other versions contain only one answer to each question)
DOI: 10.6084/m9.figshare.13295831.v17
title: Autism Spectrum Disorder and Asperger Syndrome Question Answering Dataset 1.0

description: <em><strong>RUS</strong></em> <strong>Последнее обновление: 23/05/2022</strong> Набор данных предназначен для разработки русскоязычных диалоговых систем (чат-ботов, вопросно-ответных систем и т. д.) о расстройствах аутистического спектра. Источник текстов: https://aspergers.ru Проект реализуется победителем конкурса «Практики личной филантропии и альтруизма» Благотворительного фонда Владимира Потанина. <strong>Состав набора данных:</strong> 1. original.json: оригинальная версия датасета 2. multiple.json: версия датасета с несколькими вариантами ответа 3. short.json: версия датасета с укороченными ответами 4. half_sized.json: версия датасета содержит 50% собранных данных 5. no_impossible.json: версия содержит только релевантные вопросы 7. age_dataset.tsv: набор данных для определения возраста пользователя (можно использовать для кастомизации моделей) <em><strong>ENG</strong></em> A dataset for question-answering used for building an informational Russian language chatbot for the inclusion of people with autism spectrum disorder and Asperger syndrome in particular, based on data from the following website: https://aspergers.ru. <strong>The dataset has several versions:</strong> 1. Original version 2. Half-sized version (50% of the original data) 3. No impossible version (a version without irrelevant/impossible questions) 4. Short version (a version with shorterned answers) 5. Multiple version (a version with several answers, all the other versions contain only one answer to each question)
DOI: 10.25678/000055
title: Bottom-up identification of subsystems in Swiss water governance

description: Anonymized data and R scripts needed to replicate the identification of subsystems in Swiss water governance described in the study "Bottom-up identification of subsystems in complex governance systems". This is a paper output of the SNF funded project "Overlapping subsystems". Access to the complete, non-anonymized dataset is restricted.