cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.648037
title: CIL:39121

description: High magnification freeze-fracture image of a barley (Hordeum vulgare) chloroplast from a mutant lacking chlorophyll b illustrating the changes from wildtype in particle distribution on the PFs and EFs thylakoid membrane faces. The wildtype distribution is illustrated in CIL image 39118.
DOI: 10.6084/m9.figshare.648038.v1
title: CIL:39125

description: Thin section of a wildtype chloroplast of Arabidopsis thaliana showing the characteristic distribution of thylakoids membranes in stacked grana (black arrow) and unstacked stromal regions (white arrows). The other image in this group (CIL 39126) shows the result of deleting the FZL protein.
DOI: 10.6084/m9.figshare.648039
title: CIL:39126

description: Thin section of a chloroplast of Arabidopsis thaliana lacking the FZL protein, a member of the dynamin superfamily of proteins. FZL is related to FZO, a protein that mediates fusion between mitochondrial outer membrages in animals and fungi. The characteristic distribution of thylakoids membranes in stacked grana (black arrow) and unstacked stromal regions (white arrows) was disorganized with grana lamellae less uniform in length and stacked in a staggered fashion. Asterisks denote vesicle-like structures that occur in the absence of FZL. The other image in this group (CIL 39125) shows the wildtype structure.
DOI: 10.6084/m9.figshare.648040.v1
title: CIL:39143

description: Cells of the symbiotic prochlorophyte Prochloron didemni were separated from its ascidian partner, thylakoids isolated, treated to liberate chlorophyll-containing complexes, and negatively stained preparations observed by transmission EM. The image shows an averaged top view of a chlorophyll-photsystem II super complex. The overview shown in CIL 39146 illustrates the range of particle types in the preparation.
DOI: 10.6084/m9.figshare.648035
title: CIL:38651

description: This photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green) illustrates that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. A lower magnification view that orients the adhesions on actin bundles is available as CIL 38652. Bar is 1 micron. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648036
title: CIL:38652

description: This image combines total internal reflection microscopy (TIRF) of mCerulean-actin (blue) with photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green). Vinculin and paxillin are organized into focal adhesions at the ends of actin bundles. A higher magnification of several adhesion complexes (CIL 38651) clearly shows that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648037.v1
title: CIL:39121

description: High magnification freeze-fracture image of a barley (Hordeum vulgare) chloroplast from a mutant lacking chlorophyll b illustrating the changes from wildtype in particle distribution on the PFs and EFs thylakoid membrane faces. The wildtype distribution is illustrated in CIL image 39118.
DOI: 10.6084/m9.figshare.648039.v1
title: CIL:39126

description: Thin section of a chloroplast of Arabidopsis thaliana lacking the FZL protein, a member of the dynamin superfamily of proteins. FZL is related to FZO, a protein that mediates fusion between mitochondrial outer membrages in animals and fungi. The characteristic distribution of thylakoids membranes in stacked grana (black arrow) and unstacked stromal regions (white arrows) was disorganized with grana lamellae less uniform in length and stacked in a staggered fashion. Asterisks denote vesicle-like structures that occur in the absence of FZL. The other image in this group (CIL 39125) shows the wildtype structure.
DOI: 10.6084/m9.figshare.648034
title: CIL:38600

description: Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-actin (green) and tdEos-paxillin (red). Paxillin assembles in fibrillar-like adhesions that run parallel to ventral actin fibers and cluster near the cell periphery to form larger adhesion complexes. The differential interference contrast image (DIC) corresponding to this field is CIL 38601. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13 and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648035.v1
title: CIL:38651

description: This photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green) illustrates that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. A lower magnification view that orients the adhesions on actin bundles is available as CIL 38652. Bar is 1 micron. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648032
title: CIL:38597

description: This differential interference contrast image (DIC) corresponds to the same image field as the diffraction limited TIRF image CIL 38599 and the 2-color photoactivation localization microscopy (PALM) image 38598. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648033
title: CIL:38599

description: This total internal reflection (TIRF) image of both the Dronpa-alphaa ctinin and the unconverted tdEos-vinculin channels corresponds to the same image field as the diffraction limited DIC image CIL 38597 and the 2-color photoactivation localization microscopy (PALM) image 38598. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648034.v1
title: CIL:38600

description: Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-actin (green) and tdEos-paxillin (red). Paxillin assembles in fibrillar-like adhesions that run parallel to ventral actin fibers and cluster near the cell periphery to form larger adhesion complexes. The differential interference contrast image (DIC) corresponding to this field is CIL 38601. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13 and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648036.v1
title: CIL:38652

description: This image combines total internal reflection microscopy (TIRF) of mCerulean-actin (blue) with photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green). Vinculin and paxillin are organized into focal adhesions at the ends of actin bundles. A higher magnification of several adhesion complexes (CIL 38651) clearly shows that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648030
title: CIL:37329

description: The video shows a cytoplast that is adhering to a sub-stratum in its central portion. It is also connected by a retraction fiber to its central cell. The cytoplast is engaging in vigorous protrusive activity around its periphery. The small lamellipodia is undergoing ruffling--extensions, folding back, and re-entry of the the protrusion into the main process of the cell. This demonstrates that an anucleate fragment of a cell has the capacity to nucleate actin, engage in ruffling, and retrograde flows. CIL 37336 and 37337 show that these fragments have the capacity to undergo directional locomotion. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.648031
title: CIL:37404

description: A 2.75 hour Zebrafish embryo; the same embryo as in CIL 37403 --later in blastoderm development. The video shows dynamics in a perisagital section of the embryo. The sequence shows blastomeres dividing asynchronously. The surface cells begin to take on epithelial characteristics. A distinct yolk syncytial layer emerges. At each prophase, rapid movements of yolk platelets occur within the yolk. This video made available by Mark Cooper thru Zebrafish - The Living Laboratory. The videos in this group can also be found in the Developmental Landmarks page of the Fishscope web site.
DOI: 10.6084/m9.figshare.648032.v1
title: CIL:38597

description: This differential interference contrast image (DIC) corresponds to the same image field as the diffraction limited TIRF image CIL 38599 and the 2-color photoactivation localization microscopy (PALM) image 38598. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648033.v1
title: CIL:38599

description: This total internal reflection (TIRF) image of both the Dronpa-alphaa ctinin and the unconverted tdEos-vinculin channels corresponds to the same image field as the diffraction limited DIC image CIL 38597 and the 2-color photoactivation localization microscopy (PALM) image 38598. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
DOI: 10.6084/m9.figshare.648028
title: CIL:36775

description: Cross section of the oral region (buccal cavity) posterior to the vestibulum. The cytopharynx is beginning on the dorsal surface and the ribbed wall, peniculi and quadrulus are present on the right and left. The floor of the buccal cavity is bordered by the filamentous reticulum and an anarchic field of basal bodies is found where the endoral membranelle terminates. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 6,150X. Bar = 1µm.
DOI: 10.6084/m9.figshare.648029
title: CIL:36783

description: Detail of the tubular extension of the alveolus to the cytostomal cord and the whiskers that attach it to the cord. TEM taken on 4/10/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg.18,500X. Bar = 0.2µm.
DOI: 10.6084/m9.figshare.648031.v1
title: CIL:37404

description: A 2.75 hour Zebrafish embryo; the same embryo as in CIL 37403 --later in blastoderm development. The video shows dynamics in a perisagital section of the embryo. The sequence shows blastomeres dividing asynchronously. The surface cells begin to take on epithelial characteristics. A distinct yolk syncytial layer emerges. At each prophase, rapid movements of yolk platelets occur within the yolk. This video made available by Mark Cooper thru Zebrafish - The Living Laboratory. The videos in this group can also be found in the Developmental Landmarks page of the Fishscope web site.
DOI: 10.6084/m9.figshare.648027
title: CIL:36741

description: Immunogold label specific for L1 antigen on DV-II. Monoclonal antibody to antigen L1 specifically labels DV-II and acidosomes but not lysosomes. Lysosomes are osmotically active so they tend to shrink when the cell is exposed to hyperosmotic fixative as occurred here. This vacuole was 5-8 min. old and contained 0.8µm latex beads. TEM taken on 3/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.
DOI: 10.6084/m9.figshare.648029.v1
title: CIL:36783

description: Detail of the tubular extension of the alveolus to the cytostomal cord and the whiskers that attach it to the cord. TEM taken on 4/10/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg.18,500X. Bar = 0.2µm.
DOI: 10.6084/m9.figshare.648030.v1
title: CIL:37329

description: The video shows a cytoplast that is adhering to a sub-stratum in its central portion. It is also connected by a retraction fiber to its central cell. The cytoplast is engaging in vigorous protrusive activity around its periphery. The small lamellipodia is undergoing ruffling--extensions, folding back, and re-entry of the the protrusion into the main process of the cell. This demonstrates that an anucleate fragment of a cell has the capacity to nucleate actin, engage in ruffling, and retrograde flows. CIL 37336 and 37337 show that these fragments have the capacity to undergo directional locomotion. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.648027.v1
title: CIL:36741

description: Immunogold label specific for L1 antigen on DV-II. Monoclonal antibody to antigen L1 specifically labels DV-II and acidosomes but not lysosomes. Lysosomes are osmotically active so they tend to shrink when the cell is exposed to hyperosmotic fixative as occurred here. This vacuole was 5-8 min. old and contained 0.8µm latex beads. TEM taken on 3/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.
DOI: 10.6084/m9.figshare.648023
title: CIL:36613

description: The P-face of the ciliary membrane at the base of the cilium has 9 triple rows of IMPs in register with the 9 doublets of the internal axoneme. It is thought that these IMPs may be sites of calcium influx into the base of the cilium for regulating ciliary activity. Also, trichocyst docking sites. TEM taken on 1/6/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39317) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648024
title: CIL:36614

description: In addition to plates the plasma membrane contains IMPs on its P-fracture face in ring or parenthesis shape at sites of trichocyst secretion. A ring actually consists of two circles of IMPs and signifies that a trichocyst is docked underneath while a parenthesis means the site has no docked trichocyst. The fracture also exposed some of the E-face of the outer alveolar membrane which is relatively free of IMPs. TEM taken on 6/4/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39318) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648024.v1
title: CIL:36614

description: In addition to plates the plasma membrane contains IMPs on its P-fracture face in ring or parenthesis shape at sites of trichocyst secretion. A ring actually consists of two circles of IMPs and signifies that a trichocyst is docked underneath while a parenthesis means the site has no docked trichocyst. The fracture also exposed some of the E-face of the outer alveolar membrane which is relatively free of IMPs. TEM taken on 6/4/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39318) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648025
title: CIL:36616

description: Views of a striated band linked to the epiplasm on the ridge slopes and the intermembrane space between the plasma membrane and alveolus space inside the alveolus. TEM taken on 6/6/91 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. There is a high resolution version of this image in the library (CIL:39320) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648026
title: CIL:36617

description: Quick-freeze deep-etch of granulo-fibrillar material at the crests of the ridges. This material is composed of thinner and shorter filaments that, like the striated bands, appear to bind to the membrane. The fracture plane also exposed the cell’s exterior. TEM taken on 6/4/91 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39321) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648022
title: CIL:36612

description: A row of large IMPs bordering parts of the plates are visible. Two parentheses-shaped empty trichocyst docking sites are also present and one ring surrounding pits where the rosette IMPs have been removed. TEM taken on 5/9/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39316) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648023.v1
title: CIL:36613

description: The P-face of the ciliary membrane at the base of the cilium has 9 triple rows of IMPs in register with the 9 doublets of the internal axoneme. It is thought that these IMPs may be sites of calcium influx into the base of the cilium for regulating ciliary activity. Also, trichocyst docking sites. TEM taken on 1/6/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39317) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648025.v1
title: CIL:36616

description: Views of a striated band linked to the epiplasm on the ridge slopes and the intermembrane space between the plasma membrane and alveolus space inside the alveolus. TEM taken on 6/6/91 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. There is a high resolution version of this image in the library (CIL:39320) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648026.v1
title: CIL:36617

description: Quick-freeze deep-etch of granulo-fibrillar material at the crests of the ridges. This material is composed of thinner and shorter filaments that, like the striated bands, appear to bind to the membrane. The fracture plane also exposed the cell’s exterior. TEM taken on 6/4/91 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39321) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648019
title: CIL:36605

description: Freeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous plates of IMPs in the P-face of the plasma membrane. Rings and rosette IMPs at the trichocyst exocytic sites are also seen. TEM taken on 10/2/80 by R. Allen with HU 11A operating at 75kV. Neg. 10,000X. Bar = 1µm. There is a high resolution version of this image in the library (CIL:39313) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648020
title: CIL:36607

description: In quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing between rows of 25nm. These plaques remain in the P-fracture face of the plasma membrane and are found mostly on the anterior ventral surface of the cell near the anterior suture. Their function is not known. For discussion see Allen, J. Ultrastruc. Res. 63:64-78, 1978 and Hufnagel, Micros. Res. Tech. 22:225-264, 1992. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39314) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648021
title: CIL:36610

description: In this image a portion of the true surface of the plasma membrane is exposed where the culture medium has been etched away. The IMPs of the plates can also be viewed less distinctly on the true surface of the cell. The edge of the fractured and removed E leaflet is visible. Fractured doublets of the axoneme of a cilium lie nearby. TEM taken on 6/4/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2 µm. There is a high resolution version of this image in the library (CIL:39315) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648019.v1
title: CIL:36605

description: Freeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous plates of IMPs in the P-face of the plasma membrane. Rings and rosette IMPs at the trichocyst exocytic sites are also seen. TEM taken on 10/2/80 by R. Allen with HU 11A operating at 75kV. Neg. 10,000X. Bar = 1µm. There is a high resolution version of this image in the library (CIL:39313) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648020.v1
title: CIL:36607

description: In quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing between rows of 25nm. These plaques remain in the P-fracture face of the plasma membrane and are found mostly on the anterior ventral surface of the cell near the anterior suture. Their function is not known. For discussion see Allen, J. Ultrastruc. Res. 63:64-78, 1978 and Hufnagel, Micros. Res. Tech. 22:225-264, 1992. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39314) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648021.v1
title: CIL:36610

description: In this image a portion of the true surface of the plasma membrane is exposed where the culture medium has been etched away. The IMPs of the plates can also be viewed less distinctly on the true surface of the cell. The edge of the fractured and removed E leaflet is visible. Fractured doublets of the axoneme of a cilium lie nearby. TEM taken on 6/4/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2 µm. There is a high resolution version of this image in the library (CIL:39315) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.648022.v1
title: CIL:36612

description: A row of large IMPs bordering parts of the plates are visible. Two parentheses-shaped empty trichocyst docking sites are also present and one ring surrounding pits where the rosette IMPs have been removed. TEM taken on 5/9/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39316) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).