cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.3259915
title: The influence of biomechanical properties and cannabinoids on tumor invasion

description: Background: Cannabinoids are known to have an anti-tumorous effect, but the underlying mechanisms are only sparsely understood. Mechanical characteristics of tumor cells represent a promising marker to distinguish between tumor cells and the healthy tissue. We tested the hypothesis whether cannabinoids influence the tumor cell specific mechanical and migratory properties and if these factors are a prognostic marker for the invasiveness of tumor cells. Methods: 3 different glioblastoma cell lines were treated with cannabinoids and changes of mechanical and migratory properties of single cells were measured using atomic force microscopy and time lapse imaging. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. Results: We found that cannabinoids are capable of influencing migratory and mechanical properties in a cell line specific manner. A network analysis revealed a correlation between a “generalized stiffness” and the invasiveness for all tumor cell lines after 3 and 4 d of invasion time: <i>r</i><sub><i>3d</i></sub> = −<i>0.88 [−0.52;−0.97]; r</i><sub><i>4d</i></sub> = −<i>0.90 [−0.59;−0.98]</i>. Conclusions: Here we could show that a “generalized stiffness” is a profound marker for the invasiveness of a tumor cell population in our model and thus might be of high clinical relevance for drug testing. Additionally cannabinoids were shown to be of potential use for therapeutic approaches of glioblastoma.
DOI: 10.6084/m9.figshare.3261007.v1
title: A Case Study of Potential Human Health Impacts from Petroleum Coke Transfer Facilities

description: Petroleum coke or “petcoke” is a solid material created during petroleum refinement is distributed via transfer facilities that may be located in densely populated areas. The health impacts from petcoke exposure to residents living in proximity to such facilities was evaluated for a petcoke transfer facilities located in Chicago, IL. Site-specific, Margin of Safety (MOS) and Margin of Exposure (MOE) analyses were conducted using estimated airborne and dermal exposures. Our exposure assessment was based on a combined measurement and modeling program that included multi-year on-site air monitoring, air dispersion modeling, and analyses of soil and surfaces in residential areas adjacent to two petcoke transfer facilities located in industrial areas. Airborne particles less than 10 microns (PM<sub>10</sub>) were used as a marker for petcoke. Based on daily fence line monitoring, the average daily PM<sub>10</sub> concentration at the KCBX Terminals measured on-site was 32 μg/m<sup>3</sup> with 89% of 24-hr average PM<sub>10</sub> concentrations below 50 μg/m<sup>3</sup> and 99% below 100 μg/m<sup>3</sup>. A dispersion model estimated that the emissions sources at the KCBX Terminals produced peak PM<sub>10</sub> levels attributed to the petcoke facility at the most highly impacted residence of 11 μg/m<sup>3</sup> on an annual average basis and 54 μg/m<sup>3</sup> on 24-hr average basis. Chemical indicators of petcoke in soil and surface samples collected from residential neighborhoods adjacent to the facilities were equivalent to levels in corresponding samples collected at reference locations elsewhere in Chicago; a finding that is consistent with limited potential for off-site exposure indicated by the fence line monitoring and air dispersion modeling. The MOE based upon dispersion model estimates, ranged from 800 – 900 for potential inhalation, the primary route of concern for particulate matter. This indicates a low likelihood of adverse health effects in the surrounding community. ImplicationsHandling of petroleum coke at bulk material transfer facilities has been identified as a concern for the public health of surrounding populations. Our assessment based on measurements and modeling of two facilities located in a densely populated urban area indicate that petcoke transport and accumulation in off-site locations is minimal. In addition, estimated human exposures, if any, are well below levels that could be anticipated to produce adverse health effects in the general population. Handling of petroleum coke at bulk material transfer facilities has been identified as a concern for the public health of surrounding populations. Our assessment based on measurements and modeling of two facilities located in a densely populated urban area indicate that petcoke transport and accumulation in off-site locations is minimal. In addition, estimated human exposures, if any, are well below levels that could be anticipated to produce adverse health effects in the general population.
DOI: 10.6084/m9.figshare.3259981
title: Transient overexpression of exogenous APOBEC3A causes C-to-U RNA editing of thousands of genes

description: APOBEC3A cytidine deaminase induces site-specific C-to-U RNA editing of hundreds of genes in monocytes exposed to hypoxia and/or interferons and in pro-inflammatory macrophages. To examine the impact of APOBEC3A overexpression, we transiently expressed APOBEC3A in HEK293T cell line and performed RNA sequencing. APOBEC3A overexpression induces C-to-U editing at more than 4,200 sites in transcripts of 3,078 genes resulting in protein recoding of 1,110 genes. We validate recoding RNA editing of genes associated with breast cancer, hematologic neoplasms, amyotrophic lateral sclerosis, Alzheimer disease and primary pulmonary hypertension. These results highlight the fundamental impact of APOBEC3A overexpression on human transcriptome by widespread RNA editing.
DOI: 10.6084/m9.figshare.3259981.v1
title: Transient overexpression of exogenous APOBEC3A causes C-to-U RNA editing of thousands of genes

description: APOBEC3A cytidine deaminase induces site-specific C-to-U RNA editing of hundreds of genes in monocytes exposed to hypoxia and/or interferons and in pro-inflammatory macrophages. To examine the impact of APOBEC3A overexpression, we transiently expressed APOBEC3A in HEK293T cell line and performed RNA sequencing. APOBEC3A overexpression induces C-to-U editing at more than 4,200 sites in transcripts of 3,078 genes resulting in protein recoding of 1,110 genes. We validate recoding RNA editing of genes associated with breast cancer, hematologic neoplasms, amyotrophic lateral sclerosis, Alzheimer disease and primary pulmonary hypertension. These results highlight the fundamental impact of APOBEC3A overexpression on human transcriptome by widespread RNA editing.
DOI: 10.6084/m9.figshare.3259915.v2
title: The influence of biomechanical properties and cannabinoids on tumor invasion

description: Background: Cannabinoids are known to have an anti-tumorous effect, but the underlying mechanisms are only sparsely understood. Mechanical characteristics of tumor cells represent a promising marker to distinguish between tumor cells and the healthy tissue. We tested the hypothesis whether cannabinoids influence the tumor cell specific mechanical and migratory properties and if these factors are a prognostic marker for the invasiveness of tumor cells. Methods: 3 different glioblastoma cell lines were treated with cannabinoids and changes of mechanical and migratory properties of single cells were measured using atomic force microscopy and time lapse imaging. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. Results: We found that cannabinoids are capable of influencing migratory and mechanical properties in a cell line specific manner. A network analysis revealed a correlation between a “generalized stiffness” and the invasiveness for all tumor cell lines after 3 and 4 d of invasion time: <i>r</i><sub><i>3d</i></sub> = −<i>0.88 [−0.52;−0.97]; r</i><sub><i>4d</i></sub> = −<i>0.90 [−0.59;−0.98]</i>. Conclusions: Here we could show that a “generalized stiffness” is a profound marker for the invasiveness of a tumor cell population in our model and thus might be of high clinical relevance for drug testing. Additionally cannabinoids were shown to be of potential use for therapeutic approaches of glioblastoma.
DOI: 10.6084/m9.figshare.3259915.v1
title: The Influence of Biomechanical Properties and Cannabinoids on Tumor Invasion

description: <b>Background</b>: Cannabinoids are known to have an anti-tumorous effect, but the underlying mechanisms are only sparsely understood. Mechanical characteristics of tumor cells represent a promising marker to distinguish between tumor cells and the healthy tissue. We tested the hypothesis whether cannabinoids influence the tumor cell specific mechanical and migratory properties and if these factors are a prognostic marker for the invasiveness of tumor cells. <b>Methods</b>: 3 different glioblastoma cell lines were treated with cannabinoids and changes of mechanical and migratory properties of single cells were measured using atomic force microscopy and time lapse imaging. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. <b>Results</b>: We found that cannabinoids are capable of influencing migratory and mechanical properties in a cell line specific manner. A network analysis revealed a correlation between a “generalized stiffness” and the invasiveness for all tumor cell lines after 3 and 4 days of invasion time: <i>r</i><sub><i>3d</i></sub> =−<i>0.88</i> [−0.52;−0.97]; r<sub><i>4d</i></sub> =−<i>0.90</i> [−0.59;−0.98]. <b>Conclusions</b>: Here we could show that a “generalized stiffness” is a profound marker for the invasiveness of a tumor cell population in our model and thus might be of high clinical relevance for drug testing. Additionally cannabinoids were shown to be of potential use for therapeutic approaches of glioblastoma.
DOI: 10.6084/m9.figshare.3258955
title: Use of etendue and energy spread to assess inverse-Compton X-ray sources for applications

description: While brightness is commonly used as an X-ray source figure of merit, it is not appropriate for many applications. Usable X-ray flux, restricted via application-specific etendue and energy spread, is a superior figure of merit. We present a methodology and software for calculating the usable X-ray flux for an application.
DOI: 10.6084/m9.figshare.3258784
title: outreach.csv

description: Random sample of 1000 patients from the (SPOT)light study to be used for teaching purposes.
DOI: 10.6084/m9.figshare.3258784.v1
title: outreach.csv

description: Random sample of 1000 patients from the (SPOT)light study to be used for teaching purposes.
DOI: 10.6084/m9.figshare.3255556
title: Supplementary tables of FJ Ruiz-Ruano's PhD Thesis.

description: Big tables of the FJ Ruiz-Ruano's PhD Thesis.<br>
DOI: 10.6084/m9.figshare.3258955.v2
title: Use of etendue and energy spread to assess inverse-Compton X-ray sources for applications

description: While brightness is commonly used as an X-ray source figure of merit, it is not appropriate for many applications. Usable X-ray flux, restricted via application-specific etendue and energy spread, is a superior figure of merit. We present a methodology and software for calculating the usable X-ray flux for an application.
DOI: 10.6084/m9.figshare.3258955.v1
title: Use of etendue and energy spread to assess inverse-Compton X-ray sources for applications

description: While brightness is commonly used as an X-ray source figure of merit, it is not appropriate for many applications. Usable X-ray flux, restricted via application-specific etendue and energy spread, is a superior figure of merit. We present a methodology and software for calculating the usable X-ray flux for an application.
DOI: 10.6084/m9.figshare.3254689
title: Association of Inhaled Corticosteroids with Incident Pneumonia and Mortality in COPD Patients; Systematic Review and Meta-Analysis

description: <i>Background:</i> Inhaled corticosteroids are commonly prescribed for patients with severe COPD. They have been associated with increased risk of pneumonia but not with increased pneumonia-associated or overall mortality. <i>Methods:</i> To further examine the effects of inhaled corticosteroids on pneumonia incidence, and mortality in COPD patients, we searched for potentially relevant articles in PubMed, Medline, CENTRAL, EMBASE, Scopus, Web of Science and manufacturers' web clinical trial registries from 1994 to February 4, 2014. Additionally, we checked the included and excluded studies' bibliographies. We subsequently performed systematic review and meta-analysis of included randomized controlled trials and observational studies on the topic. <i>Results:</i> We identified 38 studies: 29 randomized controlled trials and nine observational studies. The estimated unadjusted risk of pneumonia was increased in randomized trials: RR 1.61; 95% CI 1.35–1.93, <i>p</i> &lt; 0.001; as well as in observational studies: OR 1.89; 95% CI 1.39–2.58, <i>p</i> &lt; 0·001. Six randomized trials and seven observational studies were useful in estimating unadjusted risk of pneumonia ­case-fatality: RR 0.91; 95% CI 0.52–1.59, <i>p</i> = 0.74; and OR 0.72; 95% CI 0.59–0.88, <i>p</i> = 0.001, respectively. Twenty-nine randomized trials and six observational studies allowed estimation of unadjusted risk of overall mortality: RR 0.95; 95% CI 0.85–1.05, <i>p</i> = 0.31; and OR 0.79; 95% CI 0.65–0.97, <i>p</i> = 0.02, respectively. <i>Conclusions:</i> Despite a substantial and significant increase in unadjusted risk of pneumonia associated with inhaled corticosteroid use, pneumonia fatality and overall mortality were found not to be increased in randomized controlled trials and were decreased in observational studies.
DOI: 10.6084/m9.figshare.3255556.v1
title: Table_S1_revised.xls

description: Description of the microsatellites in the genome of the grasshoppers Eyprepocnemis plorans and Locusta migratoria<br>
DOI: 10.6084/m9.figshare.3252628
title: Prevalence and risk factors of infertility at a rural site of Northern China

description: This is a cross-sectional study. We conducted a face-to-face questionnaire survey from July 2014 to October 2014 involving 4,232 women who were at childbearing age in Suizhong, a medium-sized, representative county located in Northern China.A total of 22 villages from Suizhong County were investigated in this survey. This study was approved by the regional ethical committee.
DOI: 10.6084/m9.figshare.3254689.v1
title: Association of Inhaled Corticosteroids with Incident Pneumonia and Mortality in COPD Patients; Systematic Review and Meta-Analysis

description: <i>Background:</i> Inhaled corticosteroids are commonly prescribed for patients with severe COPD. They have been associated with increased risk of pneumonia but not with increased pneumonia-associated or overall mortality. <i>Methods:</i> To further examine the effects of inhaled corticosteroids on pneumonia incidence, and mortality in COPD patients, we searched for potentially relevant articles in PubMed, Medline, CENTRAL, EMBASE, Scopus, Web of Science and manufacturers' web clinical trial registries from 1994 to February 4, 2014. Additionally, we checked the included and excluded studies' bibliographies. We subsequently performed systematic review and meta-analysis of included randomized controlled trials and observational studies on the topic. <i>Results:</i> We identified 38 studies: 29 randomized controlled trials and nine observational studies. The estimated unadjusted risk of pneumonia was increased in randomized trials: RR 1.61; 95% CI 1.35–1.93, <i>p</i> &lt; 0.001; as well as in observational studies: OR 1.89; 95% CI 1.39–2.58, <i>p</i> &lt; 0·001. Six randomized trials and seven observational studies were useful in estimating unadjusted risk of pneumonia ­case-fatality: RR 0.91; 95% CI 0.52–1.59, <i>p</i> = 0.74; and OR 0.72; 95% CI 0.59–0.88, <i>p</i> = 0.001, respectively. Twenty-nine randomized trials and six observational studies allowed estimation of unadjusted risk of overall mortality: RR 0.95; 95% CI 0.85–1.05, <i>p</i> = 0.31; and OR 0.79; 95% CI 0.65–0.97, <i>p</i> = 0.02, respectively. <i>Conclusions:</i> Despite a substantial and significant increase in unadjusted risk of pneumonia associated with inhaled corticosteroid use, pneumonia fatality and overall mortality were found not to be increased in randomized controlled trials and were decreased in observational studies.
DOI: 10.6084/m9.figshare.3255556.v2
title: Microsats_eplo_lmig_sup_tables

description: Description of the microsatellites in the genome of the grasshoppers Eyprepocnemis plorans and Locusta migratoria<br>
DOI: 10.6084/m9.figshare.3250540
title: Comprehensive species-level dietary data for Class Mammalia

description: <b>See ReadMe file for a full description of the data.</b><br>In addition to the data we have uploaded two additional files:(1) the full R script (Olalla-Tarraga_et_al_R_script.R) used to generate the results reported in the publication associated with these data "Miguel Á. Olalla-Tárraga, Manuela González-Suárez, Rubén Bernardo-Madrid, Eloy Revilla, Fabricio Villalobos. 2016. Contrasting evidence of phylogenetic trophic niche conservatism in mammals worldwide. <i>Journal of Biogeography</i>. In press"<br>(2) The mammalian phylogeny used in the analyses (Fritz_tree.nex) generated by Fritz, S. A., Bininda-Emonds, O. R. P., & Purvis, A. (2009). Geographical variation in predictors of mammalian extinction risk: big is bad, but only in the tropics. Ecology Letters, 12, 538-549<br>
DOI: 10.6084/m9.figshare.3250534
title: The number of UNESCO World Heritage sites by country and national statistics

description: Dataset with the number of UNESCO World Heritage sites across countries and nation variables: GDP, population and area.
DOI: 10.6084/m9.figshare.3250159
title: Database Speech ABR Otitis.xlsx

description: Research about speech ABR and otite
DOI: 10.6084/m9.figshare.3252628.v1
title: primary data.xls

description: This is a cross-sectional study. We conducted a face-to-face questionnaire survey from July 2014 to October 2014 involving 4,232 women who were at childbearing age in Suizhong, a medium-sized, representative county located in Northern China.A total of 22 villages from Suizhong County were investigated in this survey. This study was approved by the regional ethical committee.
DOI: 10.6084/m9.figshare.3255556.v3
title: Suplementary tables of FJ Ruiz-Ruano's PhD Thesis.

description: Big tables of the FJ Ruiz-Ruano's PhD Thesis.<br>
DOI: 10.6084/m9.figshare.3255556.v4
title: Suplementary tables of FJ Ruiz-Ruano's PhD Thesis.

description: Big tables of the FJ Ruiz-Ruano's PhD Thesis.<br>
DOI: 10.6084/m9.figshare.3250159.v1
title: Database Speech ABR Otite.xlsx

description: Research about speech ABR and otite
DOI: 10.6084/m9.figshare.3250534.v1
title: UWH_stats.csv

description: Dataset with the number of UNESCO World Heritage sites across countries and nation variables: GDP, population and area.
DOI: 10.6084/m9.figshare.3255556.v5
title: Suplementary tables of FJ Ruiz-Ruano's PhD Thesis.

description: Big tables of the FJ Ruiz-Ruano's PhD Thesis.<br>
DOI: 10.6084/m9.figshare.3249400
title: Germline variants in Hamartomatous Polyposis Syndrome-associated genes from patients with one or few hamartomatous polyps

description: <b>Objective:</b> A subgroup of patients with hamartomatous polyps in the GI tract has a hereditary Hamartomatous Polyposis Syndrome with an increased risk of cancer. The distinction between patients with one or few polyps and patients with a syndrome can be difficult. A pathogenic germline mutation can be detected in a majority of HPS patients. This study investigates whether patients with one or few hamartomatous polyps could have a syndrome based on genetic screening of relevant genes. <b>Methods:</b> We designed a gene panel including 26 hamartomatous polyposis-associated genes. Using targeted Next Generation Sequencing, DNA samples from 77 patients with 84 hamartomatous polyps were sequenced. The detected germline variants were classified into pathogenicity classes. <b>Results:</b> We detected several germline variants, among them three in <i>ENG</i>, two in <i>BMPR1A</i>, one in <i>PTEN</i>, and one in <i>SMAD4.</i> Although some of the detected variants have been reported previously none could be definitely pathogenic or likely pathogenic. <b>Conclusions:</b> Our study points towards that genetic testing for the Hamartomatous Polyposis Syndromes in patients with one or few polyps does not improve diagnostics, however we illustrate that the clinical significance of genetic variants can be difficult to interpret. A family history of polyps, cancer, or extraintestinal findings or a minimum of 3–5 polyps seems to be relevant information to include before genetic testing.
DOI: 10.6084/m9.figshare.3255556.v6
title: Suplementary tables of FJ Ruiz-Ruano's PhD Thesis.

description: Big tables of the FJ Ruiz-Ruano's PhD Thesis.<br>
DOI: 10.6084/m9.figshare.3249400.v1
title: Germline variants in Hamartomatous Polyposis Syndrome-associated genes from patients with one or few hamartomatous polyps

description: <b>Objective:</b> A subgroup of patients with hamartomatous polyps in the GI tract has a hereditary Hamartomatous Polyposis Syndrome with an increased risk of cancer. The distinction between patients with one or few polyps and patients with a syndrome can be difficult. A pathogenic germline mutation can be detected in a majority of HPS patients. This study investigates whether patients with one or few hamartomatous polyps could have a syndrome based on genetic screening of relevant genes. <b>Methods:</b> We designed a gene panel including 26 hamartomatous polyposis-associated genes. Using targeted Next Generation Sequencing, DNA samples from 77 patients with 84 hamartomatous polyps were sequenced. The detected germline variants were classified into pathogenicity classes. <b>Results:</b> We detected several germline variants, among them three in <i>ENG</i>, two in <i>BMPR1A</i>, one in <i>PTEN</i>, and one in <i>SMAD4.</i> Although some of the detected variants have been reported previously none could be definitely pathogenic or likely pathogenic. <b>Conclusions:</b> Our study points towards that genetic testing for the Hamartomatous Polyposis Syndromes in patients with one or few polyps does not improve diagnostics, however we illustrate that the clinical significance of genetic variants can be difficult to interpret. A family history of polyps, cancer, or extraintestinal findings or a minimum of 3–5 polyps seems to be relevant information to include before genetic testing.
DOI: 10.6084/m9.figshare.3255556.v8
title: Suplementary tables of FJ Ruiz-Ruano's PhD Thesis.

description: Big tables of the FJ Ruiz-Ruano's PhD Thesis.<br>