DOI: 10.5061/dryad.1c59zw3xp
title: Independence of mate choice components (choosiness and preference functions) in Hyla versicolor

description: Mate choice is an important cause of natural and sexual selection, driving
 the evolution of ornaments and promoting diversification and speciation.
 Mate choice decisions arise from the interaction of several components,
 and knowledge of whether they interact, and how, is crucial for
 understanding their contributions to selection. Here we focus on the
 relationship between preference functions (attractiveness ranking of
 prospective mates) and choosiness (effort invested in obtaining the
 preferred mate) and test the hypothesis that they are independent
 components of mate choice decisions. We examine individual variation in
 preference functions and choosiness for call duration in female Hyla
 versicolor treefrogs, and show that measures describing preference
 functions and choosiness are not correlated. We also found a suggestive
 but inconclusive pattern that both components are influenced by different
 factors (body measures and hormones). Independence of preference and
 choosiness suggests that the joint study of variation in both components
 is required to gain a complete understanding of how mate choice
 contributes to sexual selection and speciation.
description: There are three different csv files associated with one another:
 'PFuncData', 'PREDICTION_1', and
 'PREDICTION_2'. Also accompanying this data is a readme
 file. <strong>PFunc Data
 --</strong> Collected using acoustic single-speaker playback trials
 with Eastern Gray Treefrogs (Hyla versicolor). We played simulated
 artificial male calls of varying duration on speakers broadcast to female
 frogs. Each female was presented 8 different stimuli (pulse numbers=6, 9,
 12, 18, 21, 24, 27) during 8 different behavioral trials. A female was
 given a maximum of 5 minutes to choose a male call (approached speaker
 within 10cm). Time to choose was recorded, a faster response indicating a
 more attractive stimulus.  Recorded on this data sheet
 is (1) female ID in columns A and J, (2) the latency to choose (sec) the
 speaker in columns B-I, and (3) latency to choose subtracted from 5min in
 columns K-R. Data was transformed (K-R) for input into the program PFunc
 (https://github.com/joccalor/pfunc) for a more intuitive interpretation. <strong>PREDICTION 1 -- </strong>This data is partially derived from the raw data (methods described above) in the 'PFunc' file, the preference function traits. This data was uploaded into the program PFunc (https://github.com/joccalor/pfunc) to gather four different measures on this csv file: Peak (C), Tolerance (D), Strength (E), and Responsiveness (F). Also included are year of collection (A) and female ID (B). Peak indicates the most attractive stimulus, and the other traits generally explain how the preference function deviates from the peak.  We also include here a different measure of mate choice, choosiness, that indicates the amount of energy a female is willing to invest for the most attractive mate. Although we still use playbacks with artificial male call stimuli, we use a two speaker design with only two stimuli (6 pulse and 18 pulse) played at different amplitudes. By manipulating amplitude, we change the percieved distance from the call, so a quieter call=further away. The choosiness data here indicates the maximum amplitude difference between stimuli (the quietest dB in which the attractive stimulus was chosen). <strong>PREDICTION 2 -- </strong>This file examines the relationships between possible influencing variables and the mate choice traits explained above. These include (B) body size, snout-vent-length, (C) condition, regression between mass and SVL, (D) clutch size, (F) testosterone, (G) corticosterone, and the mate choice measures (H-L). Hormone concentrations were measured using ELIZA assays on salivary samples and clutch size was counted using photos and ImageJ (subset of photos uploaded here).
description: Uploaded ReadMe file with more detailed explanations of the three
 csv data sheets.