DOI: 10.5061/dryad.b2rbnzss8
title: Data from: Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation potential and facilitate tendon regeneration

description: Tendon injury occurs at high frequency and is difficult to repair.
 Identification of human stem cells being able to regenerate tendon will
 greatly facilitate the development of regenerative medicine for tendon
 injury. Genetic and functional analyses identify human CD29⁺/CD56⁺
 myogenic progenitors with tenogenic differentiation potential in vitro and
 in vivo. Transplantation of human CD29+/CD56+ myogenic progenitors
 contributes to injured tendon repair and thus improves locomotor function.
 Interestingly, the tendon differentiation potential in mouse muscle stem
 cells is minimal and the higher TGFβ signaling level may be the key for
 the distinct feature of human CD29+/CD56+ myogenic progenitors. The
 discovery of bi-potential CD29⁺/CD56⁺ myogenic progenitors highlight their
 potential as a novel adult stem cell source for tendon regeneration.
description: # Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation
 potential and facilitate tendon regeneration Dataset DOI:
 [10.5061/dryad.b2rbnzss8](10.5061/dryad.b2rbnzss8) ## Description of the
 data and file structure Data of RT-qPCR results, immunofluorescence
 staining, bio-mechanical test, force test, and treadmill exercise test.
 ### Files and variables #### File: fig1e.xlsx **Description:** Proportion
 of CD29+/CD56+ myogenic progenitors in a specific cell population #####
 Variables * CD29+/CD56+ myogenic progenitors * Total CD29+/CD56+ cells *
 Total myogenic progenitors #### File: fig1g.xlsx
 **Description:** Statistical analysis of the percentage of PAX7+, MYOD1+,
 and MYF5+ cells in the isolated CD29+/CD56+ myogenic progenitors. #####
 Variables * PAX7+, MYOD1+ and MYF5+ cells * DAPI+ cells #### File:
 fig1h.xlsx **Description:** Analysis of cell proliferation for human
 CD29+/CD56+ myogenic progenitors in vitro. 10,000 isolated human
 CD29+/CD56+ myogenic progenitors were plated for proliferation and counted
 at each time point. ##### Variables * Cell counts at different times. ####
 File: fig1j.xlsx **Description:** Percentage of nuclei in MyHC+ myotubes
 after differentiation of the human CD29+/CD56+ myogenic progenitors. #####
 Variables * Total DAPI+ cells * MyHC+ DAPI #### File: fig1k\_l.xlsx
 **Description:** Relative expression level of *PAX7*, *MYF5*, *MYOD1,
 MYOG*, *MYH1*, and *MYH3* in human CD29+/CD56+ myogenic progenitors
 (Undifferentiated) and differentiated myotubes (Differentiated). #####
 Variables * CT (cycle threshold) value * Delta Ct value * Delta Delta Ct
 value * fold change #### File: fig2b.xlsx **Description:** Quantifying TNC
 and SCX fluorescent intensity in human CD29+/CD56+ myogenic progenitors
 has undergone myogenic and tenogenic differentiation, respectively. #####
 Variables * TNC fluorescent intensity * SCX fluorescent intensity ####
 File: fig2g.xlsx **Description:** Statistical analysis of the myogenic and
 tenogenic differentiation efficiency of human CD29+/CD56+ myogenic
 progenitors. ##### Variables * MyHC-positive wells * MyHC negative wells *
 SCX positive wells * SCX negative wells #### File: fig2c.xlsx
 **Description:** Relative expression levels of genes enriched in tendon
 cells. RT-qPCR assays were performed with human CD29+/CD56+ myogenic
 progenitors upon myogenic and tenogenic differentiation, respectively.
 GAPDH served as a reference gene. ##### Variables * CT value * Relative
 Quantification (RQ) in fold change * CT Mean value #### File: fig4b.xlsx
 **Description:** Percentage of nuclei in MyHC+ myotubes. ##### Variables *
 MyHC negative nuclei * Total nuclei #### File: fig4c.xlsx
 **Description:** Relative expression levels of myogenic
 differentiation-specific genes *MyoG*, *Myh1*, and *Myh3*. Mouse MuSCs
 were induced to differentiate into muscle and tendon, respectively. Mouse
 MuSCs before and after differentiation, together with primary tenocytes,
 were harvested and subjected to RT-qPCR analysis. *Gapdh* served as a
 reference gene. ##### Variables * CT value * Fold change #### File:
 fig4d.xlsx **Description:** Relative expression levels of tenogenic
 differentiation marker genes *Scx*, *Tnc*, *Col I*, *Mkx,* and *Thbs4*.
 Mouse MuSCs were induced to differentiate into muscle and tendon,
 respectively. Mouse MuSCs before and after differentiation, together with
 primary tenocytes, were harvested and subjected to RT-qPCR analysis.
 *Gapdh* served as a reference gene. ##### Variables * CT value * Fold
 change #### File: fig4g.xlsx **Description:** Percentage of tdTomato+ TNC+
 cells 4 months after tendon injury. ##### Variables * TNC+ tdTomato+ MyHC-
 cells * Total cells #### File: fig5g.xlsx **Description:** TNC+ tdTomato+
 cells in tendon tissue after transplantation of murine MuSCs. #####
 Variables * TNC+/Tdtomato+/MyHC- cells * Total cells #### File: fig5d.xlsx
 **Description:** Percentage of human cells expressing skeletal muscle
 marker MyHC or tendon marker TNC after being transplanted to the injured
 tendon. ##### Variables * TNC+ Lamin AC+ cells * Lamin AC+ cells * MyHC+
 Lamin AC+ cells * Lamin AC+ cells1 #### File: fig6b.xlsx
 **Description:** Collagen fibril diameter and percentage of collagen
 fibril area for the injured tendon with PBS injection, injured tendon with
 human CD29+/CD56+ myogenic progenitors transplantation, and uninjured
 tendon. ##### Variables * Collagen fibril diameter  * Percentage of
 collagen fibril area #### File: fig6d.xlsx **Description:** The results of
 twitch and tetanus plantarflexion force of the involved limb with PBS
 injection after tendon injury, human CD29+/CD56+ myogenic progenitors
 transplantation after tendon injury, and uninjured tendon. ##### Variables
 * Twitch torque * Tetanus torque #### File: fig6e.xlsx
 **Description:** The results of treadmill exercise for tendon-injured mice
 with or without human CD29+/CD56+ myogenic progenitors transplantation.
 ##### Variables * Endurance time * Max fatigue speed #### File: fig6c.xlsx
 **Description:** The max load and stiffness of the injured tendon with PBS
 injection, the injured tendon with human CD29+/CD56+ myogenic progenitors
 transplantation, and the uninjured tendon. ##### Variables * Max load *
 Stiffness #### File: fig7e.xlsx **Description:** Relative expression
 levels of tendon-related genes. RT-qPCR assays were performed with human
 CD29+/CD56+ myogenic progenitors upon tenogenic differentiation with or
 without TGFβ signaling pathway inhibitor SB-431542 for 12 days,
 respectively. GAPDH served as a reference gene. ##### Variables * CT value
 * Fold change #### File: fig7g.xlsx **Description:** Relative expression
 levels of muscle-related genes. RT-qPCR assays were performed with human
 CD29+/CD56+ myogenic progenitors upon tenogenic differentiation with or
 without TGFβ signaling pathway inhibitor SB-431542 for 12 days,
 respectively. GAPDH served as a reference gene. ##### Variables * CT value
 * Fold change The differential expression analysis for RNA-seq data
 between human CD29+/CD56+ myogenic progenitors and mouse MuSCs was
 performed according to previous literature (PMID: 30925894). ## Access
 information Other publicly accessible locations of the data: * NA Data was
 derived from the following sources: * NA