cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19899236
title: Effects of small-scale anthropogenic alterations in Euglossini bees at eastern Amazon forest

description: ABSTRACT In this study, through a rapid assessment, we evaluated differences in richness, abundance, species composition and beta diversity of Euglossini bees in preserved areas and areas altered by riverine human populations in the Caxiuanã National Forest (northern Amazon). Our hypotheses were: i) the species richness, diversity, abundance and composition of Euglossini bees differ between the preserved and altered areas because many species are intolerant to small anthropic disturbances; and ii) areas altered by riverine populations have higher values of beta diversity (species dissimilarity between sites) than preserved areas, due to local changes in habitat and increased heterogeneity between sites. We sampled two areas (preserved and altered) and, in each area, four transects were sampled with odorous baits. We used a Student’s t test to evaluate differences in richness, abundance, and Shannon-Wiener diversity index. We also evaluated differences in composition and differences in beta diversity between areas. We collected 102 specimens, identified in 18 species and three genera, 63 individuals (61.8% of the total) in preserved areas and 39 individuals (38.2%) in altered areas. Euglossa liopoda (Dressler, 1982) was the most abundant in altered areas and Euglossa imperialis (Cockerell, 1922) the most abundant in preserved areas. Species richness was 2.5 times greater in the transects of preserved areas, and species composition also differed between the sampled areas. In contrast, species abundance did not differ between transects of both areas. Despite our small sample size, this study demonstrated that the richness, composition and beta diversity of Euglossini bees can be potentially affected by local changes in habitat caused by small-scale activities provided by traditional Amazonian groups. Our results indicate the urgency for more comprehensive studies aimed to investigate these relationships.
DOI: 10.6084/m9.figshare.19899236.v1
title: Effects of small-scale anthropogenic alterations in Euglossini bees at eastern Amazon forest

description: ABSTRACT In this study, through a rapid assessment, we evaluated differences in richness, abundance, species composition and beta diversity of Euglossini bees in preserved areas and areas altered by riverine human populations in the Caxiuanã National Forest (northern Amazon). Our hypotheses were: i) the species richness, diversity, abundance and composition of Euglossini bees differ between the preserved and altered areas because many species are intolerant to small anthropic disturbances; and ii) areas altered by riverine populations have higher values of beta diversity (species dissimilarity between sites) than preserved areas, due to local changes in habitat and increased heterogeneity between sites. We sampled two areas (preserved and altered) and, in each area, four transects were sampled with odorous baits. We used a Student’s t test to evaluate differences in richness, abundance, and Shannon-Wiener diversity index. We also evaluated differences in composition and differences in beta diversity between areas. We collected 102 specimens, identified in 18 species and three genera, 63 individuals (61.8% of the total) in preserved areas and 39 individuals (38.2%) in altered areas. Euglossa liopoda (Dressler, 1982) was the most abundant in altered areas and Euglossa imperialis (Cockerell, 1922) the most abundant in preserved areas. Species richness was 2.5 times greater in the transects of preserved areas, and species composition also differed between the sampled areas. In contrast, species abundance did not differ between transects of both areas. Despite our small sample size, this study demonstrated that the richness, composition and beta diversity of Euglossini bees can be potentially affected by local changes in habitat caused by small-scale activities provided by traditional Amazonian groups. Our results indicate the urgency for more comprehensive studies aimed to investigate these relationships.
DOI: 10.6084/m9.figshare.20097572
title: Physiographic analysis of the Atibaia River Basin and flood susceptibility mapping in the municipality of Campinas-SP, Brazil

description: Abstract This study characterized and analyzed the physiographic factors of the Atibaia River Basin and their influence on flooding processes and evaluated the susceptibility to flooding in the municipality of Campinas during the years 1985 and 2019. At first, geoprocessing techniques and indices defined in the literature were used to provide a comprehensive understanding of the Atibaia River Basin regarding its geometric, relief, and drainage attributes and its influence on flooding processes, to provide decision-making support regarding preventive and mitigating actions for the socioeconomic and environmental impacts in the region. The pedogeomorphological characteristics and land use and occupation of Campinas were applied to the Analytic Hierarchy Process using geoprocessing techniques to generate the specific mapping of the municipality’s susceptibility to flooding. The mapping allowed us to identify the critical sites of the municipality of Campinas, choosing priority areas for government action and programs regarding urban and environmental management. It can also guide new studies on the detail scale aiming to prevent and mitigate flooding events.
DOI: 10.6084/m9.figshare.20097572.v1
title: Physiographic analysis of the Atibaia River Basin and flood susceptibility mapping in the municipality of Campinas-SP, Brazil

description: Abstract This study characterized and analyzed the physiographic factors of the Atibaia River Basin and their influence on flooding processes and evaluated the susceptibility to flooding in the municipality of Campinas during the years 1985 and 2019. At first, geoprocessing techniques and indices defined in the literature were used to provide a comprehensive understanding of the Atibaia River Basin regarding its geometric, relief, and drainage attributes and its influence on flooding processes, to provide decision-making support regarding preventive and mitigating actions for the socioeconomic and environmental impacts in the region. The pedogeomorphological characteristics and land use and occupation of Campinas were applied to the Analytic Hierarchy Process using geoprocessing techniques to generate the specific mapping of the municipality’s susceptibility to flooding. The mapping allowed us to identify the critical sites of the municipality of Campinas, choosing priority areas for government action and programs regarding urban and environmental management. It can also guide new studies on the detail scale aiming to prevent and mitigate flooding events.
DOI: 10.6084/m9.figshare.20097574
title: THE USE OF SURGICAL ADHESIVE AND SUTURE FIXING MESHES TO THE ABDOMINAL WALL: AN EXPERIMENTAL STUDY IN RATS

description: ABSTRACT - BACKGROUND: Proper fixation of the surgical mesh determines the success of a herniorrhaphy. Understanding the inflammatory response and the mechanical properties of the mesh helps to define whether a fixation method is superior. AIM: This study aimed to evaluate the healing of defects in the abdominal wall of rats, comparing the repair of macroporous polypropylene meshes fixed with surgical glue and polypropylene thread. METHODS: In 20 Wistar rats, a defect was produced in the abdominal wall, with the integrity of the parietal peritoneum. For correction, the meshes were fixed with surgical glue (2-octyl cyanoacrylate) (subgroup C1), or polypropylene suture (subgroup C2). The two subgroups of 10 animals were euthanized on the 90th postoperative day, and the fragments of the abdominal wall were submitted to macroscopic, histological, and tensiometric analysis. RESULTS: Macroscopic analysis did not show any abnormalities. Tensiometry on the 90th postoperative day in subgroup C1 showed mean rupture tension of 28.47N and in subgroup C2 32.06N (p=0.773). The inflammatory process score revealed that both groups are in the subacute phase (p=0.380). CONCLUSION: The fixation of a polypropylene macroporous mesh to repair an abdominal wall defect can be performed with surgical glue (2-octyl cyanoacrylate) or polypropylene suture, both methods being equally effective.
DOI: 10.6084/m9.figshare.20097574.v1
title: THE USE OF SURGICAL ADHESIVE AND SUTURE FIXING MESHES TO THE ABDOMINAL WALL: AN EXPERIMENTAL STUDY IN RATS

description: ABSTRACT - BACKGROUND: Proper fixation of the surgical mesh determines the success of a herniorrhaphy. Understanding the inflammatory response and the mechanical properties of the mesh helps to define whether a fixation method is superior. AIM: This study aimed to evaluate the healing of defects in the abdominal wall of rats, comparing the repair of macroporous polypropylene meshes fixed with surgical glue and polypropylene thread. METHODS: In 20 Wistar rats, a defect was produced in the abdominal wall, with the integrity of the parietal peritoneum. For correction, the meshes were fixed with surgical glue (2-octyl cyanoacrylate) (subgroup C1), or polypropylene suture (subgroup C2). The two subgroups of 10 animals were euthanized on the 90th postoperative day, and the fragments of the abdominal wall were submitted to macroscopic, histological, and tensiometric analysis. RESULTS: Macroscopic analysis did not show any abnormalities. Tensiometry on the 90th postoperative day in subgroup C1 showed mean rupture tension of 28.47N and in subgroup C2 32.06N (p=0.773). The inflammatory process score revealed that both groups are in the subacute phase (p=0.380). CONCLUSION: The fixation of a polypropylene macroporous mesh to repair an abdominal wall defect can be performed with surgical glue (2-octyl cyanoacrylate) or polypropylene suture, both methods being equally effective.
DOI: 10.6084/m9.figshare.20097577
title: Binding of the extracellular matrix laminin-1 to Clostridioides difficile strains

description: BACKGROUND Clostridioides difficile is the most common cause of nosocomial diarrhea associated with antibiotic use. The disease’s symptoms are caused by enterotoxins, but other surface adhesion factors also play a role in the pathogenesis. These adhesins will bind to components of extracellular matrix. OBJECTIVE There is a lack of knowledge on MSCRAMM, this work set-out to determine the adhesive properties of several C. difficile ribotypes (027, 133, 135, 014, 012) towards laminin-1 (LMN-1). METHODS A binding experiment revealed that different ribotypes have distinct adhesion capabilities. To identify this adhesin, an affinity chromatography column containing LMN-1 was prepared and total protein extracts were analysed using mass spectrometry. FINDINGS Strains from ribotypes 012 and 027 had the best adhesion when incubated with glucose supplementations (0.2%, 0.5%, and 1%), while RT135 had a poor adherence. The criteria were not met by RT014 and RT133. In the absence of glucose, there was no adhesion for any ribotype, implying that glucose is required and plays a significant role in adhesion. MAIN CONCLUSIONS These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein.
DOI: 10.6084/m9.figshare.20097577.v1
title: Binding of the extracellular matrix laminin-1 to Clostridioides difficile strains

description: BACKGROUND Clostridioides difficile is the most common cause of nosocomial diarrhea associated with antibiotic use. The disease’s symptoms are caused by enterotoxins, but other surface adhesion factors also play a role in the pathogenesis. These adhesins will bind to components of extracellular matrix. OBJECTIVE There is a lack of knowledge on MSCRAMM, this work set-out to determine the adhesive properties of several C. difficile ribotypes (027, 133, 135, 014, 012) towards laminin-1 (LMN-1). METHODS A binding experiment revealed that different ribotypes have distinct adhesion capabilities. To identify this adhesin, an affinity chromatography column containing LMN-1 was prepared and total protein extracts were analysed using mass spectrometry. FINDINGS Strains from ribotypes 012 and 027 had the best adhesion when incubated with glucose supplementations (0.2%, 0.5%, and 1%), while RT135 had a poor adherence. The criteria were not met by RT014 and RT133. In the absence of glucose, there was no adhesion for any ribotype, implying that glucose is required and plays a significant role in adhesion. MAIN CONCLUSIONS These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein.
DOI: 10.6084/m9.figshare.20097580
title: Does vitamin C have benefit in schizophrenia? Preliminary study with behavioral analysis in rats

description: ABSTRACT Objective The objective of this study was to analyze in rats models of schizophrenia, induced by the administration of ketamine, the possibility of the beneficial effects of ascorbic acid in schizophrenia, through the behavioral test. Methods This preliminary study was simple blind and randomized. The ketamine psychosis protocol was carried out in 24 Wistar rats, which were subsequently submitted to behavioral analysis. The first group was supplied with: water and ketamine; the second: ascorbic acid and ketamine; the third: antipsychotic, ascorbic acid and ketamine; the fourth: antipsychotic, water and ketamine. Behavioral analysis was performed using the Open Field Test, considered the standard method for assessing behavior in animal models of schizophrenia. Statistical analysis was performed by the IBM-SPSS software, using the Generalized Linear Models. Results Treatment in haloperidol monotherapy (Quadrant Average: 44.5 ± 15.8; CI: 13.54-75.46/Center Average: 2.67 ± 0.67; CI: 1.63-4.35) and combined with vitamin C (Quadrant Average: 38.67 ± 15.8; CI: 7.71-69.52/Center Average: 2.00 ± 0.58; CI: 1.14-3.52), demonstrated benefits in the animal model of schizophrenia induced by ketamine (Quadrant Average: 108.5 ± 15.8; CI: 77.54-139.46/Center Average: 11.33 ± 1.37; CI: 8.94- 14.37) (p &lt; 0.001). The isolated treatment with vitamin C did not show a significant result (Quadrant Average: 62.00 ± 15.8; CI: 31.04-92.96/Mean Average: 7.00 ± 1.08; CI: 5.17-9.47). Conclusion The association of antipsychotic and vitamin C and only the antipsychotic demonstrated a therapeutic effect respecting to controls. Vitamin C manage separately had no benefit.
DOI: 10.6084/m9.figshare.20097580.v1
title: Does vitamin C have benefit in schizophrenia? Preliminary study with behavioral analysis in rats

description: ABSTRACT Objective The objective of this study was to analyze in rats models of schizophrenia, induced by the administration of ketamine, the possibility of the beneficial effects of ascorbic acid in schizophrenia, through the behavioral test. Methods This preliminary study was simple blind and randomized. The ketamine psychosis protocol was carried out in 24 Wistar rats, which were subsequently submitted to behavioral analysis. The first group was supplied with: water and ketamine; the second: ascorbic acid and ketamine; the third: antipsychotic, ascorbic acid and ketamine; the fourth: antipsychotic, water and ketamine. Behavioral analysis was performed using the Open Field Test, considered the standard method for assessing behavior in animal models of schizophrenia. Statistical analysis was performed by the IBM-SPSS software, using the Generalized Linear Models. Results Treatment in haloperidol monotherapy (Quadrant Average: 44.5 ± 15.8; CI: 13.54-75.46/Center Average: 2.67 ± 0.67; CI: 1.63-4.35) and combined with vitamin C (Quadrant Average: 38.67 ± 15.8; CI: 7.71-69.52/Center Average: 2.00 ± 0.58; CI: 1.14-3.52), demonstrated benefits in the animal model of schizophrenia induced by ketamine (Quadrant Average: 108.5 ± 15.8; CI: 77.54-139.46/Center Average: 11.33 ± 1.37; CI: 8.94- 14.37) (p &lt; 0.001). The isolated treatment with vitamin C did not show a significant result (Quadrant Average: 62.00 ± 15.8; CI: 31.04-92.96/Mean Average: 7.00 ± 1.08; CI: 5.17-9.47). Conclusion The association of antipsychotic and vitamin C and only the antipsychotic demonstrated a therapeutic effect respecting to controls. Vitamin C manage separately had no benefit.
DOI: 10.6084/m9.figshare.19996772.v1
title: Analysis of wideband tympanometry in Ménière’s disease

description: Abstract Introduction: Endolymphatic hydrops is the pathophysiological substrate of Ménière’s disease. The changes in the inner ear, transmitted to the middle ear through changes in the ossicular chain mobility, can be quantified by wideband tympanometry, through the measurement of the acoustic absorbance at multiple frequencies, represented by the sound energy absorbed by the middle ear, even at its early stages. Studying the behavior of the middle ear through the absorbance in patients with endolymphatic hydrops under ambient pressure and under peak pressure can be useful for detecting Ménière’s disease. Objective: To characterize acoustic absorbance behavior in subjects with symptomatic and asymptomatic Ménière’s disease compared to controls, in order to verify the ability of wideband tympanometry to detect Ménière’s disease. Methods: We carried out a cross-sectional study with a diagnostic approach comparing the findings of wideband tympanometry at ambient pressure and peak pressure between the ears of the control group (n = 30), the asymptomatic group (n = 21) and the symptomatic group (n = 9). Results: Different peak pressure values were found between the ears of the control group (0daPa), the asymptomatic group (−11 daPa) and the symptomatic group (−192 daPa), with p &lt; 0.05 by the Kruskal-Wallis test, Mann Whitney test and Bonferroni correction. Different absorbance values were found between the ears of the symptomatic group and the asymptomatic group compared to the control group for low frequencies at ambient pressure and peak pressure, with p &lt; 0.05 by the Kruskal-Wallis test, Mann Whitney test and Bonferroni correction. Conclusions: The Wideband Tympanometry test was capable of identifying the presence of Ménières disease, and to differentiate between asymptomatic and symptomatic patients, when comparing them with healthy individuals.
DOI: 10.6084/m9.figshare.19996772
title: Analysis of wideband tympanometry in Ménière’s disease

description: Abstract Introduction: Endolymphatic hydrops is the pathophysiological substrate of Ménière’s disease. The changes in the inner ear, transmitted to the middle ear through changes in the ossicular chain mobility, can be quantified by wideband tympanometry, through the measurement of the acoustic absorbance at multiple frequencies, represented by the sound energy absorbed by the middle ear, even at its early stages. Studying the behavior of the middle ear through the absorbance in patients with endolymphatic hydrops under ambient pressure and under peak pressure can be useful for detecting Ménière’s disease. Objective: To characterize acoustic absorbance behavior in subjects with symptomatic and asymptomatic Ménière’s disease compared to controls, in order to verify the ability of wideband tympanometry to detect Ménière’s disease. Methods: We carried out a cross-sectional study with a diagnostic approach comparing the findings of wideband tympanometry at ambient pressure and peak pressure between the ears of the control group (n = 30), the asymptomatic group (n = 21) and the symptomatic group (n = 9). Results: Different peak pressure values were found between the ears of the control group (0daPa), the asymptomatic group (−11 daPa) and the symptomatic group (−192 daPa), with p &lt; 0.05 by the Kruskal-Wallis test, Mann Whitney test and Bonferroni correction. Different absorbance values were found between the ears of the symptomatic group and the asymptomatic group compared to the control group for low frequencies at ambient pressure and peak pressure, with p &lt; 0.05 by the Kruskal-Wallis test, Mann Whitney test and Bonferroni correction. Conclusions: The Wideband Tympanometry test was capable of identifying the presence of Ménières disease, and to differentiate between asymptomatic and symptomatic patients, when comparing them with healthy individuals.
DOI: 10.6084/m9.figshare.20097583
title: CCR6 expression reduces mouse survival upon malarial challenge with Plasmodium berghei NK65 strain

description: BACKGROUND It has been demonstrated that proteins expressed by liver-stage Plasmodium parasites can inhibit the translocation of transcription factors to the nucleus of different cells. This process would hinder the expression of immune genes, such as the CCL20 chemokine. OBJECTIVE Since CCR6 is the only cognate receptor for CCL20, we investigated the importance of this chemokine-receptor axis against rodent malaria. METHODS CCR6-deficient (KO) and wild-type (WT) C57BL/6 mice were challenged with Plasmodium berghei (Pb) NK65 sporozoites or infected red blood cells (iRBCs). Liver parasitic cDNA, parasitemia and serum cytokine concentrations were respectively evaluated through reverse transcription-polymerase chain reaction (RT-PCR), staining thin-blood smears with Giemsa solution, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Although the sporozoite challenges yielded similar liver parasitic cDNA and parasitemia, KO mice presented a prolonged survival than WT mice. After iRBC challenges, KO mice kept displaying higher survival rates as well as a decreased IL-12 p70 concentration in the serum than WT mice. CONCLUSION Our data suggest that malaria triggered by PbNK65 liver- or blood-stage forms elicit a pro-inflammatory environment that culminates with a decreased survival of infected C57BL/6 mice.
DOI: 10.6084/m9.figshare.20097583.v1
title: CCR6 expression reduces mouse survival upon malarial challenge with Plasmodium berghei NK65 strain

description: BACKGROUND It has been demonstrated that proteins expressed by liver-stage Plasmodium parasites can inhibit the translocation of transcription factors to the nucleus of different cells. This process would hinder the expression of immune genes, such as the CCL20 chemokine. OBJECTIVE Since CCR6 is the only cognate receptor for CCL20, we investigated the importance of this chemokine-receptor axis against rodent malaria. METHODS CCR6-deficient (KO) and wild-type (WT) C57BL/6 mice were challenged with Plasmodium berghei (Pb) NK65 sporozoites or infected red blood cells (iRBCs). Liver parasitic cDNA, parasitemia and serum cytokine concentrations were respectively evaluated through reverse transcription-polymerase chain reaction (RT-PCR), staining thin-blood smears with Giemsa solution, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Although the sporozoite challenges yielded similar liver parasitic cDNA and parasitemia, KO mice presented a prolonged survival than WT mice. After iRBC challenges, KO mice kept displaying higher survival rates as well as a decreased IL-12 p70 concentration in the serum than WT mice. CONCLUSION Our data suggest that malaria triggered by PbNK65 liver- or blood-stage forms elicit a pro-inflammatory environment that culminates with a decreased survival of infected C57BL/6 mice.