cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19248437
title: Stability and utility of flow cytometric platelet activation tests: A modality to bridge the gap between diagnostic demand and supply

description: Light transmission aggregometry (LTA) is the gold standard for the diagnosis of platelet function disorders (PFDs). The requirement of customized aggregometer, large blood volume, normal platelet count and processing within 4 hours of venipuncture for LTA makes platelet function testing inaccessible to wider population. Flow cytometric platelet activation test (PACT) may overcome these limitations. This study compares the performance of PACT with LTA, characterizes diagnostic patterns of PFDs on PACT and assesses the stability of PACT beyond 4 hours of venipuncture in controls (n = 5) at different temperature conditions. LTA and PACT were performed in 121 healthy controls and 66 patients with suspected PFD. PACT had excellent agreement (kappa = 0.93) with LTA and 94.1% sensitivity, 98.5% specificity. PACT had distinct patterns in Bernard Soulier Syndrome (n = 10), Glanzmann Thrombasthenia (n = 24), δ-granule disorder (n = 7), and other PFDs (n = 12). PACT could assess platelet function in patients (14%) with thrombocytopenia/lipemia wherein LTA was inconclusive. PACT was stable up to 24 hours in samples stored/transported at 2–8◦C. The results of utility and stability are only valid for the specific markers, agonist concentrations, and conditions investigated in this paper. PACT is a useful modality for the diagnosis of PFD, especially in children, thrombocytopenia cases or in the setup where an aggregometer is not readily available.
DOI: 10.6084/m9.figshare.19355050.v2
title: The role of miRNAs in regulation of platelet activity and related diseases - a bioinformatic analysis

description: MicroRNAs (miRNAs) are small, non-coding RNAs, able to regulate cellular functions by induction of mRNA degradation and post-transcriptional repression of gene expression. Platelets are the major source of circulating miRNAs, with significant regulatory potential on cardiovascular pathophysiology and other diseases. MiRNAs have been shown to modify the expression of platelet proteins, which influence the platelets reactivity. Circulating miRNAs can be determined from plasma, serum, or whole blood, and they can be used as diagnostic and prognostic biomarkers as well as therapeutic targets including cardiovascular diseases (CVDs). Herein, we present original results from bioinformatic analyses, which identified top 22 platelet-related miRNAs including hsa-miR-320a, hsa-miR-16-5p, hsa-miR-106a-5p, hsa-miR-320b, hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-92a-3p as widely involved in platelet reactivity and associated diseases, including CVDs, Alzheimer’s and cerebrovascular diseases, cancer and hypertension. Analysis focused on the identification of the highly regulatory targets shared between those miRNAs identified 43 of them. Best ranked genes associated with overall platelet activity and most susceptible for noncoding regulation were PTEN, PIK3R1, CREB1, APP, and MAPK1. Top targets also strongly associated with CVDs were VEGFA, IGF1, ESR1, BDNF, and PPARG. Top targets associated with other platelet-related diseases including cancer identified in our study were TP53, KRAS, and CCND1. The most affected pathways by top miRNAs and top targets included diseases of signal transduction by Growth Factor Receptors (GDFRs) and second messengers, platelet activation, signaling, and aggregation, signaling by VEGF, MAPK family signaling cascades, and signaling by Interleukins. Terms specific only for platelet-related miRNAs included coronary artery disease, platelet degranulation, and neutrophil degranulation, while for the top platelet-related genes it was Estrogen Signaling Receptor (ESR) mediated signaling, extra-nuclear estrogen signaling, and endometriosis. Our results show the novel features of platelet physiology and may provide a basis for further clinical studies focused on platelet reactivity. They also show in which aspects miRNAs can be promising biomarkers of platelet-related pathological processes.
DOI: 10.6084/m9.figshare.19355050.v1
title: The role of miRNAs in regulation of platelet activity and related diseases - a bioinformatic analysis

description: MicroRNAs (miRNAs) are small, non-coding RNAs, able to regulate cellular functions by induction of mRNA degradation and post-transcriptional repression of gene expression. Platelets are the major source of circulating miRNAs, with significant regulatory potential on cardiovascular pathophysiology and other diseases. MiRNAs have been shown to modify the expression of platelet proteins, which influence the platelets reactivity. Circulating miRNAs can be determined from plasma, serum, or whole blood, and they can be used as diagnostic and prognostic biomarkers as well as therapeutic targets including cardiovascular diseases (CVDs). Herein, we present original results from bioinformatic analyses, which identified top 22 platelet-related miRNAs including hsa-miR-320a, hsa-miR-16-5p, hsa-miR-106a-5p, hsa-miR-320b, hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-92a-3p as widely involved in platelet reactivity and associated diseases, including CVDs, Alzheimer’s and cerebrovascular diseases, cancer and hypertension. Analysis focused on the identification of the highly regulatory targets shared between those miRNAs identified 43 of them. Best ranked genes associated with overall platelet activity and most susceptible for noncoding regulation were PTEN, PIK3R1, CREB1, APP, and MAPK1. Top targets also strongly associated with CVDs were VEGFA, IGF1, ESR1, BDNF, and PPARG. Top targets associated with other platelet-related diseases including cancer identified in our study were TP53, KRAS, and CCND1. The most affected pathways by top miRNAs and top targets included diseases of signal transduction by Growth Factor Receptors (GDFRs) and second messengers, platelet activation, signaling, and aggregation, signaling by VEGF, MAPK family signaling cascades, and signaling by Interleukins. Terms specific only for platelet-related miRNAs included coronary artery disease, platelet degranulation, and neutrophil degranulation, while for the top platelet-related genes it was Estrogen Signaling Receptor (ESR) mediated signaling, extra-nuclear estrogen signaling, and endometriosis. Our results show the novel features of platelet physiology and may provide a basis for further clinical studies focused on platelet reactivity. They also show in which aspects miRNAs can be promising biomarkers of platelet-related pathological processes.
DOI: 10.6084/m9.figshare.19447439.v1
title: Contributing Role of Mitochondrial Energy Metabolism on Platelet Adhesion, Activation and Thrombus Formation under Blood Flow Conditions

description: Platelets have an active energy metabolism mediated by mitochondria. However, the role of mitochondria in platelet adhesion, activation, and thrombus formation under blood flow conditions remains to be elucidated. Blood specimens were obtained from healthy adult volunteers. The consumption of glucose molecules by platelets was measured after 24 hours. Platelet adhesion, activation, and thrombus formation on collagen fibrils and immobilized von Willebrand factor (VWF) at a wall shear rate of 1,500 s<sup>−1</sup> were detected by fluorescence microscopy with an ultrafast laser confocal unit in the presence or absence of mitochondrial functional inhibitors of carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), antimycin A, and oligomycin. Consumption of glucose molecules within the first 24 h of 4.21 × 10<sup>−15</sup> ± 4.46 x 10<sup>−15</sup> (n = 6) increased to 13.82 × 10<sup>−15</sup> ± 3.46 x 10<sup>−15</sup> (n = 4) in the presence of FCCP, 12.11 × 10<sup>−15</sup> ± 2.33 x 10<sup>−15</sup> (n = 4) in the presence of antimycin A, and 11.87 × 10<sup>−15</sup> ± 3.56 x 10<sup>−15</sup> (n = 4) in the presence of oligomycin (p &lt; .05). These mitochondrial functional blockers did not influence both surface area coverage by platelets and the 3-dimensional size of platelet thrombi formed on the collagen fibrils. However, a rapid increase in the intracellular calcium ion concentration ([Ca<sup>2+</sup>]<i><sub>i</sub></i>) upon adhering on immobilized VWF decreased significantly from 405.5 ± 86.2 nM in control to 198.0 ± 79.2 nM in the presence of FCCP (p &lt; .005). A similar decrease in the rapid increase in ([Ca<sup>2+</sup>]<i><sub>i</sub></i>) was observed in the presence of antimycin A and oligomycin. Mitochondrial function is necessary for platelet activation represented by a rapid increase in [Ca<sup>2+</sup>]<i><sub>i</sub></i> after platelet adhesion on VWF. However, the influence could not be detected as changes in platelet adhesion or 3-dimensional growth of platelet thrombi on collagen fibrils.
DOI: 10.6084/m9.figshare.19248437.v1
title: Stability and utility of flow cytometric platelet activation tests: A modality to bridge the gap between diagnostic demand and supply

description: Light transmission aggregometry (LTA) is the gold standard for the diagnosis of platelet function disorders (PFDs). The requirement of customized aggregometer, large blood volume, normal platelet count and processing within 4 hours of venipuncture for LTA makes platelet function testing inaccessible to wider population. Flow cytometric platelet activation test (PACT) may overcome these limitations. This study compares the performance of PACT with LTA, characterizes diagnostic patterns of PFDs on PACT and assesses the stability of PACT beyond 4 hours of venipuncture in controls (n = 5) at different temperature conditions. LTA and PACT were performed in 121 healthy controls and 66 patients with suspected PFD. PACT had excellent agreement (kappa = 0.93) with LTA and 94.1% sensitivity, 98.5% specificity. PACT had distinct patterns in Bernard Soulier Syndrome (n = 10), Glanzmann Thrombasthenia (n = 24), δ-granule disorder (n = 7), and other PFDs (n = 12). PACT could assess platelet function in patients (14%) with thrombocytopenia/lipemia wherein LTA was inconclusive. PACT was stable up to 24 hours in samples stored/transported at 2–8◦C. The results of utility and stability are only valid for the specific markers, agonist concentrations, and conditions investigated in this paper. PACT is a useful modality for the diagnosis of PFD, especially in children, thrombocytopenia cases or in the setup where an aggregometer is not readily available.
DOI: 10.6084/m9.figshare.19355050
title: The role of miRNAs in regulation of platelet activity and related diseases - a bioinformatic analysis

description: MicroRNAs (miRNAs) are small, non-coding RNAs, able to regulate cellular functions by induction of mRNA degradation and post-transcriptional repression of gene expression. Platelets are the major source of circulating miRNAs, with significant regulatory potential on cardiovascular pathophysiology and other diseases. MiRNAs have been shown to modify the expression of platelet proteins, which influence the platelets reactivity. Circulating miRNAs can be determined from plasma, serum, or whole blood, and they can be used as diagnostic and prognostic biomarkers as well as therapeutic targets including cardiovascular diseases (CVDs). Herein, we present original results from bioinformatic analyses, which identified top 22 platelet-related miRNAs including hsa-miR-320a, hsa-miR-16-5p, hsa-miR-106a-5p, hsa-miR-320b, hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-92a-3p as widely involved in platelet reactivity and associated diseases, including CVDs, Alzheimer’s and cerebrovascular diseases, cancer and hypertension. Analysis focused on the identification of the highly regulatory targets shared between those miRNAs identified 43 of them. Best ranked genes associated with overall platelet activity and most susceptible for noncoding regulation were PTEN, PIK3R1, CREB1, APP, and MAPK1. Top targets also strongly associated with CVDs were VEGFA, IGF1, ESR1, BDNF, and PPARG. Top targets associated with other platelet-related diseases including cancer identified in our study were TP53, KRAS, and CCND1. The most affected pathways by top miRNAs and top targets included diseases of signal transduction by Growth Factor Receptors (GDFRs) and second messengers, platelet activation, signaling, and aggregation, signaling by VEGF, MAPK family signaling cascades, and signaling by Interleukins. Terms specific only for platelet-related miRNAs included coronary artery disease, platelet degranulation, and neutrophil degranulation, while for the top platelet-related genes it was Estrogen Signaling Receptor (ESR) mediated signaling, extra-nuclear estrogen signaling, and endometriosis. Our results show the novel features of platelet physiology and may provide a basis for further clinical studies focused on platelet reactivity. They also show in which aspects miRNAs can be promising biomarkers of platelet-related pathological processes.
DOI: 10.34740/kaggle/dsv/4115750
title: 2021 World Population (updated daily)
DOI: 10.7284/149452
title: GNSS (Global Navigation Satellite System) data collected during research cruise LMG0309 using a Ashtech ADU2 instrument system onboard the platform RV Laurence M. Gould

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/149453
title: GNSS (Global Navigation Satellite System) data collected during research cruise LMG0309 using a Garmin GPS-17HVS instrument system onboard the platform RV Laurence M. Gould

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/149458
title: GNSS (Global Navigation Satellite System) data collected during research cruise LMG0309 using a Trimble Centurion P(Y) instrument system onboard the platform RV Laurence M. Gould

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/149459
title: Gyrocompass data collected during research cruise LMG0309 using a SGB Meridian instrument system onboard the platform RV Laurence M. Gould

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/117435
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise SKQ201507T using a Hawaii UHDAS instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/123142
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise SKQ201604S using a Hawaii UHDAS instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137650
title: Anemometer data collected during research cruise SKQ201605S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137651
title: Anemometer data collected during research cruise SKQ201605S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Processed data created by vessel technician during a Field_expedition
DOI: 10.7284/137804
title: Anemometer data collected during research cruise SKQ201605S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137805
title: Anemometer data collected during research cruise SKQ201605S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137495
title: Water Temperature data collected during research cruise SKQ201605S using a SeaBird SBE-38 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137646
title: Anemometer data collected during research cruise SKQ201607S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137647
title: Anemometer data collected during research cruise SKQ201607S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Processed data created by vessel technician during a Field_expedition
DOI: 10.7284/137800
title: Anemometer data collected during research cruise SKQ201607S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137801
title: Anemometer data collected during research cruise SKQ201607S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137493
title: Water Temperature data collected during research cruise SKQ201607S using a SeaBird SBE-38 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137644
title: Anemometer data collected during research cruise SKQ201608S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137645
title: Anemometer data collected during research cruise SKQ201608S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Processed data created by vessel technician during a Field_expedition
DOI: 10.7284/137798
title: Anemometer data collected during research cruise SKQ201608S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137799
title: Anemometer data collected during research cruise SKQ201608S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137492
title: Water Temperature data collected during research cruise SKQ201608S using a SeaBird SBE-38 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137642
title: Anemometer data collected during research cruise SKQ201609S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137643
title: Anemometer data collected during research cruise SKQ201609S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Processed data created by vessel technician during a Field_expedition
DOI: 10.7284/137796
title: Anemometer data collected during research cruise SKQ201609S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/137797
title: Anemometer data collected during research cruise SKQ201609S using a RMYoung 85004 instrument system onboard the platform RV Sikuliaq

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.48597/r3mc-x3my
title: Measurement of Pp_DDE at Alert

description: Measurement of Pp_DDE at Alert
DOI: 10.48597/rjcr-uhed
title: Measurement of 1-3-5-trichlorobenzene at Alert

description: Measurement of 1-3-5-trichlorobenzene at Alert
DOI: 10.48597/rpes-2mkk
title: Measurement of Gamma_HCH at Alert

description: Measurement of Gamma_HCH at Alert
DOI: 10.48597/rq89-ksap
title: Measurement of Tetrachloroveratrole at Alert

description: Measurement of Tetrachloroveratrole at Alert
DOI: 10.48597/rs3b-7zur
title: Measurement of HBCD at Alert

description: Measurement of HBCD at Alert
DOI: 10.48597/s4js-ahr5
title: Measurement of BDE_138 at Alert

description: Measurement of BDE_138 at Alert
DOI: 10.48597/s5rj-tcfg
title: Measurement of Tetrachloroveratrole at Alert

description: Measurement of Tetrachloroveratrole at Alert
DOI: 10.48597/sedz-jc5s
title: Measurement of Pentachloroanisole at Alert

description: Measurement of Pentachloroanisole at Alert
DOI: 10.48597/tcf8-h8tm
title: Measurement of 1-2-4-5-tetrachlorobenzene at Alert

description: Measurement of 1-2-4-5-tetrachlorobenzene at Alert
DOI: 10.48597/tesp-fkyn
title: Measurement of BDE_190 at Alert

description: Measurement of BDE_190 at Alert
DOI: 10.48597/u5np-9jm2
title: Measurement of BDE_85 at Alert

description: Measurement of BDE_85 at Alert
DOI: 10.48597/uep4-qafq
title: Measurement of BDE_183 at Alert

description: Measurement of BDE_183 at Alert
DOI: 10.48597/uve4-m52t
title: Measurement of BDE_190 at Alert

description: Measurement of BDE_190 at Alert
DOI: 10.48597/vccw-3b9w
title: Measurement of Endrin at Alert

description: Measurement of Endrin at Alert
DOI: 10.48597/vcez-wg3d
title: Measurement of Cis_CD at Alert

description: Measurement of Cis_CD at Alert
DOI: 10.48597/vu2v-5t39
title: Measurement of Heptachlor at Alert

description: Measurement of Heptachlor at Alert
DOI: 10.48597/vwgn-aepu
title: Measurement of Trichloroveratrole at Alert

description: Measurement of Trichloroveratrole at Alert
DOI: 10.48597/vzq7-nmaj
title: Measurement of Op_DDT at Alert

description: Measurement of Op_DDT at Alert
DOI: 10.48597/w7c7-8bu3
title: Measurement of Cis_CD at Alert

description: Measurement of Cis_CD at Alert
DOI: 10.48597/wand-pv8q
title: Measurement of 1-2-3-4-tetrachlorobenzene at Alert

description: Measurement of 1-2-3-4-tetrachlorobenzene at Alert
DOI: 10.48597/wfgk-9qhz
title: Measurement of Heptachlorepoxide at Alert

description: Measurement of Heptachlorepoxide at Alert
DOI: 10.48597/wg8m-q49a
title: Measurement of Gamma_HCH at Alert

description: Measurement of Gamma_HCH at Alert
DOI: 10.48597/wguf-b4xq
title: Measurement of Cis_CD at Alert

description: Measurement of Cis_CD at Alert
DOI: 10.48597/wjjb-9v6q
title: Measurement of BDE_85 at Alert

description: Measurement of BDE_85 at Alert
DOI: 10.48597/wk8b-w2vb
title: Measurement of 1-2-4-trichlorobenzene at Alert

description: Measurement of 1-2-4-trichlorobenzene at Alert
DOI: 10.48597/wmwq-prju
title: Measurement of 1-2-4-5-tetrachlorobenzene at Alert

description: Measurement of 1-2-4-5-tetrachlorobenzene at Alert
DOI: 10.48597/wqsk-vnnm
title: Measurement of 1-2-dichlorobenzene at Alert

description: Measurement of 1-2-dichlorobenzene at Alert
DOI: 10.48597/wrmc-fa2s
title: Measurement of BDE_100 at Alert

description: Measurement of BDE_100 at Alert
DOI: 10.48597/wvbp-d84n
title: Measurement of 1-2-4-trichlorobenzene at Alert

description: Measurement of 1-2-4-trichlorobenzene at Alert
DOI: 10.48597/wxqw-34tw
title: Measurement of BDE_190 at Alert

description: Measurement of BDE_190 at Alert
DOI: 10.48597/x5qk-fzjd
title: Measurement of HBCD at Alert

description: Measurement of HBCD at Alert
DOI: 10.48597/x96s-ez29
title: Measurement of BDE_47 at Alert

description: Measurement of BDE_47 at Alert
DOI: 10.48597/xb4f-b4ez
title: Measurement of BDE_190 at Alert

description: Measurement of BDE_190 at Alert
DOI: 10.48597/xc5w-vafn
title: Measurement of HBCD at Alert

description: Measurement of HBCD at Alert
DOI: 10.48597/xegt-qq9t
title: Measurement of Pp_DDD at Alert

description: Measurement of Pp_DDD at Alert
DOI: 10.48597/xggm-e73d
title: Measurement of Octachlorostyrene at Alert

description: Measurement of Octachlorostyrene at Alert
DOI: 10.48597/xs64-2gsk
title: Measurement of Methoxychlor at Alert

description: Measurement of Methoxychlor at Alert
DOI: 10.48597/xyzm-89py
title: Measurement of Mirex at Alert

description: Measurement of Mirex at Alert
DOI: 10.48597/y3gp-67qr
title: Measurement of BDE_138 at Alert

description: Measurement of BDE_138 at Alert
DOI: 10.48597/yjd3-3ad5
title: Measurement of Trans_CD at Alert

description: Measurement of Trans_CD at Alert