cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.t4uvjg
title: Occurrence Download

description: A dataset containing 31 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((133 11,138 11,138 23,133 23,133 11))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Echinodermata"
 ]
}

 The dataset includes 31 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0057829-251120083545085/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13130/rd_unimi/pddrps/jf7elv
title: Maggio_1937_Pagina_265.jpg

description: Bulletin of 1937/05/27
DOI: 10.13130/rd_unimi/pddrps/uk5d73
title: Maggio_1937_Pagina_266.jpg

description: Bulletin of 1937/05/27
DOI: 10.17632/vxt8bc6vnk
title: Data for "Contrasting mobility of phosphorus and arsenic in sediments of small estuaries: Insight into high-resolution profiles and seasonal variation"

description: Data for "Contrasting mobility of phosphorus and arsenic in sediments of small estuaries: Insight into high-resolution profiles and seasonal variation"
DOI: 10.13130/rd_unimi/pddrps/a4texp
title: Maggio_1937_Pagina_267.jpg

description: Bulletin of 1937/05/27
DOI: 10.17632/vxt8bc6vnk.1
title: Data for "Contrasting mobility of phosphorus and arsenic in sediments of small estuaries: Insight into high-resolution profiles and seasonal variation"

description: Data for "Contrasting mobility of phosphorus and arsenic in sediments of small estuaries: Insight into high-resolution profiles and seasonal variation"
DOI: 10.13130/rd_unimi/pddrps/uqrpor
title: Maggio_1937_Pagina_268.jpg

description: Bulletin of 1937/05/27
DOI: 10.13130/rd_unimi/pddrps/jz15gt
title: Maggio_1937_Pagina_269.jpg

description: Bulletin of 1937/05/27
DOI: 10.13130/rd_unimi/pddrps/bra7il
title: Maggio_1937_Pagina_270.jpg

description: Bulletin of 1937/05/27
DOI: 10.13130/rd_unimi/pddrps/aslfyj
title: Maggio_1937_Pagina_271.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/iftn8a
title: Maggio_1937_Pagina_272.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/n8outr
title: Maggio_1937_Pagina_273.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/qoy7zz
title: Maggio_1937_Pagina_274.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/izzvyy
title: Maggio_1937_Pagina_275.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/mpryai
title: Maggio_1937_Pagina_276.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/b2rbre
title: Maggio_1937_Pagina_277.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/ydxvzb
title: Maggio_1937_Pagina_278.jpg

description: Bulletin of 1937/05/28
DOI: 10.13130/rd_unimi/pddrps/6gy2vf
title: Maggio_1937_Pagina_279.jpg

description: Bulletin of 1937/05/28
DOI: 10.25625/apn9vh/ccxqvv
title: FaDeLive_Poster_1.pdf

description: :unav
DOI: 10.13130/rd_unimi/pddrps/nn2uyy
title: Maggio_1937_Pagina_280.jpg

description: Bulletin of 1937/05/28
DOI: 10.25625/apn9vh/gh1rud
title: FaDeLive_Poster_2.pdf

description: :unav
DOI: 10.25625/apn9vh/un49dy
title: 20250401_Metadata_FaDeLive.csv

description: :unav
DOI: 10.13130/rd_unimi/pddrps/bohc0p
title: Maggio_1937_Pagina_281.jpg

description: Bulletin of 1937/05/29
DOI: 10.15468/dl.yybtxh
title: Occurrence Download

description: A dataset containing 9185 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Opuntia fragilis"
 ]
}

 The dataset includes 9185 records from 112 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0057830-251120083545085/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25625/apn9vh/b6nijw
title: Dataset und Ausgaben.zip

description: :unav
DOI: 10.13130/rd_unimi/pddrps/osthzz
title: Maggio_1937_Pagina_282.jpg

description: Bulletin of 1937/05/29
DOI: 10.17632/r97fk636yn
title: Integrated Multi-Model Machine Learning Uncovers the Therapeutic Mechanism of Zhenqi Yangxue Formula in Alleviating Chemotherapy-Induced Myelosuppression via Targeting IL6 and GPR84

description: Western Blot (WB) Raw Data
DOI: 10.25625/apn9vh/wsghhk
title: FaDeLive_Poster_2.pdf

description: :unav
DOI: 10.25625/apn9vh/z5prkf
title: FaDeLive_Poster_1.pdf

description: :unav
DOI: 10.13130/rd_unimi/pddrps/t2be9p
title: Maggio_1937_Pagina_283.jpg

description: Bulletin of 1937/05/29
DOI: 10.17632/r97fk636yn.1
title: Integrated Multi-Model Machine Learning Uncovers the Therapeutic Mechanism of Zhenqi Yangxue Formula in Alleviating Chemotherapy-Induced Myelosuppression via Targeting IL6 and GPR84

description: Western Blot (WB) Raw Data
DOI: 10.25625/apn9vh/wf8cgg
title: FaDeLive_Poster_1.pdf

description: :unav
DOI: 10.25625/apn9vh/xwdmot
title: Dataset und Ausgaben.zip

description: :unav
DOI: 10.13130/rd_unimi/pddrps/fjps23
title: Maggio_1937_Pagina_284.jpg

description: Bulletin of 1937/05/29
DOI: 10.13130/rd_unimi/pddrps/l4dutn
title: Maggio_1937_Pagina_285.jpg

description: Bulletin of 1937/05/29
DOI: 10.25625/apn9vh/4ni7z7
title: FaDeLive_Poster_2.pdf

description: :unav
DOI: 10.25625/apn9vh/eebx7j
title: FaDeLive_Dataset_und_Ausgaben.zip

description: :unav
DOI: 10.13130/rd_unimi/pddrps/qfdtbj
title: Maggio_1937_Pagina_286.jpg

description: Bulletin of 1937/05/29
DOI: 10.17632/xdrmkby88h
title: Related data for "Reconstructing MinC-ZipA-FtsZ module for minimal cell division"

description: The uploaded file contains the WB and statistical analysis data for the figures shown in this research.
DOI: 10.25625/apn9vh/remguc
title: fadelive_exp_v1.zip

description: The .zip-File contains the metadata and corpus (in data/, sep=“;“), the processed corpus and visualisations (in output/), and for the reproduction the requirements (requirements.yml), the configuration (config/), resources and lists for nlp (resources/).
DOI: 10.25625/apn9vh/zkdmye
title: 20250602_Metadata_FaDeLive.csv

description: :unav
DOI: 10.13130/rd_unimi/pddrps/85okhp
title: Maggio_1937_Pagina_287.jpg

description: Bulletin of 1937/05/29
DOI: 10.25625/apn9vh/srv4dq
title: README.md

description: :unav
DOI: 10.13130/rd_unimi/pddrps/w6snfx
title: Maggio_1937_Pagina_288.jpg

description: Bulletin of 1937/05/29
DOI: 10.17632/xdrmkby88h.1
title: Related data for "Reconstructing MinC-ZipA-FtsZ module for minimal cell division"

description: The uploaded file contains the WB and statistical analysis data for the figures shown in this research.
DOI: 10.25625/apn9vh
title: „Lesen heißt diese Übung“ – und dann? Begriffe und intendierte Praktiken der Vermittlung deutschsprachiger Literatur an höheren Schulen 1796–1890

description: Im Verlauf des 19. Jahrhunderts etabliert sich die Vermittlung von Literatur im deutschsprachigen Unterricht an höheren Schulen. Seitdem gehört Literatur zum integralen Bestandteil des Fachs Deutsch an weiterführenden Schulen und zu ihren kanonischen Unterrichtsgegenständen.
DOI: 10.13130/rd_unimi/pddrps/gt2viu
title: Maggio_1937_Pagina_289.jpg

description: Bulletin of 1937/05/29
DOI: 10.13130/rd_unimi/pddrps/m1s49g
title: Maggio_1937_Pagina_291.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/rts2re
title: Maggio_1937_Pagina_290.jpg

description: Bulletin of 1937/05/29
DOI: 10.17632/v34v3cjmbz
title: Evaluating impacts of MGB and Exam-centric curricula on student performance in CURE project

description: Grade Outcomes for a Science for Non-STEM majors course taught using three philosophies of pedagogical curricula, a points and percentage model (PPM), a mastery based grading (MBG) model, and an exam centric grading model (ECG) which allowed for retakes of exams and involved peer review of assignments. All 13 sections had a CURE based final project.
DOI: 10.13130/rd_unimi/pddrps/kyzcsi
title: Maggio_1937_Pagina_292.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/ako3lf
title: Maggio_1937_Pagina_293.jpg

description: Bulletin of 1937/05/30
DOI: 10.17632/v34v3cjmbz.1
title: Evaluating impacts of MGB and Exam-centric curricula on student performance in CURE project

description: Grade Outcomes for a Science for Non-STEM majors course taught using three philosophies of pedagogical curricula, a points and percentage model (PPM), a mastery based grading (MBG) model, and an exam centric grading model (ECG) which allowed for retakes of exams and involved peer review of assignments. All 13 sections had a CURE based final project.
DOI: 10.13130/rd_unimi/pddrps/outd3g
title: Maggio_1937_Pagina_294.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/4xrp7d
title: Maggio_1937_Pagina_295.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/gig0kh
title: Maggio_1937_Pagina_296.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/bt5eem
title: Maggio_1937_Pagina_297.jpg

description: Bulletin of 1937/05/30
DOI: 10.17632/2grh8jvxvh
title: raw data on resileince and pediatric FGIDs study

description: This dataset represents the anonymized raw responses from a prospective three-wave longitudinal cohort study conducted in Jinghong City, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, Southwest China. The study enrolled 6,011 eligible participants initially, with a final analytical sample of 5,344 children and adolescents aged 10–17 years after exclusions for age ineligibility or incomplete data. Data collection spanned baseline (T1: December 18–29, 2023), first follow-up (T2: March 17–29, 2024), and second follow-up (T3: June 20–29, 2024), employing self-administered paper questionnaires in school classroom settings under supervised conditions.
DOI: 10.13130/rd_unimi/pddrps/dns9mi
title: Maggio_1937_Pagina_298.jpg

description: Bulletin of 1937/05/30
DOI: 10.15468/dl.bxe4p8
title: Occurrence Download

description: A dataset containing 364 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Brachidontes rodriguezii, Modiola lepida, Modiola martensi, Mytilus rodriguezii)",
 "HasCoordinate is true",
 "BasisOfRecord is one of (Observation, Machine Observation, Human Observation, Occurrence evidence)",
 "OccurrenceStatus is Present",
 "Year is greater than or equal to 1950"
 ]
}

 The dataset includes 364 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0057831-251120083545085/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.17632/2grh8jvxvh.1
title: raw data on resileince and pediatric FGIDs study

description: This dataset represents the anonymized raw responses from a prospective three-wave longitudinal cohort study conducted in Jinghong City, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, Southwest China. The study enrolled 6,011 eligible participants initially, with a final analytical sample of 5,344 children and adolescents aged 10–17 years after exclusions for age ineligibility or incomplete data. Data collection spanned baseline (T1: December 18–29, 2023), first follow-up (T2: March 17–29, 2024), and second follow-up (T3: June 20–29, 2024), employing self-administered paper questionnaires in school classroom settings under supervised conditions.
DOI: 10.13130/rd_unimi/pddrps/iyaeei
title: Maggio_1937_Pagina_299.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/cgz4cv
title: Maggio_1937_Pagina_300.jpg

description: Bulletin of 1937/05/30
DOI: 10.13130/rd_unimi/pddrps/a0y8oq
title: Maggio_1937_Pagina_301.jpg

description: Bulletin of 1937/05/31
DOI: 10.17632/cks6ytttnv
title: Original datasets of differentially expressed genes and metabolites

description: The manuscript "Multi-omics reveals an energy–redox–ribosome network controlling embryogenic decline in Schisandra chinensis: implications for sustainable micropropagation" is based on the above two datasets for data analysis and chart plotting.
DOI: 10.13130/rd_unimi/pddrps/fcktay
title: Maggio_1937_Pagina_302.jpg

description: Bulletin of 1937/05/31
DOI: 10.13130/rd_unimi/pddrps/zvihtb
title: Maggio_1937_Pagina_303.jpg

description: Bulletin of 1937/05/31
DOI: 10.17632/cks6ytttnv.1
title: Original datasets of differentially expressed genes and metabolites

description: The manuscript "Multi-omics reveals an energy–redox–ribosome network controlling embryogenic decline in Schisandra chinensis: implications for sustainable micropropagation" is based on the above two datasets for data analysis and chart plotting.
DOI: 10.13130/rd_unimi/pddrps/valt8r
title: Maggio_1937_Pagina_305.jpg

description: Bulletin of 1937/05/31
DOI: 10.13130/rd_unimi/pddrps/x007oz
title: Maggio_1937_Pagina_304.jpg

description: Bulletin of 1937/05/31
DOI: 10.13130/rd_unimi/pddrps/mdghp9
title: Maggio_1937_Pagina_306.jpg

description: Bulletin of 1937/05/31
DOI: 10.17632/35m7hts93z
title: Real or Virtual? A Study on the Impact of Government Advocates on Consumers' Green Behavior Intention

description: Test datasets for the four studies in the research paper named above. 
The table has been converted between Chinese and English and the paper' title has been revised.
Two preliminary experiments were added.
DOI: 10.13130/rd_unimi/pddrps/swohbu
title: Maggio_1937_Pagina_307.jpg

description: Bulletin of 1937/05/31
DOI: 10.17632/35m7hts93z.3
title: Real or Virtual? A Study on the Impact of Government Advocates on Consumers' Green Behavior Intention

description: Test datasets for the four studies in the research paper named above. 
The table has been converted between Chinese and English and the paper' title has been revised.
Two preliminary experiments were added.
DOI: 10.13130/rd_unimi/pddrps/yj9xr9
title: Maggio_1937_Pagina_308.jpg

description: Bulletin of 1937/05/31
DOI: 10.13130/rd_unimi/pddrps/jtevhj
title: Maggio_1937_Pagina_309.jpg

description: Bulletin of 1937/05/31
DOI: 10.13130/rd_unimi/pddrps/rpnkiy
title: Maggio_1937_Pagina_310.jpg

description: Bulletin of 1937/05/31
DOI: 10.17632/r9h9xj452x
title: Trace elemental analysis of quartz using femtosecond laser ICP-TOFMS

description: Comparison of Two Femtosecond Laser Techniques (Matrix-Array fs-LA and Conventional fs-LA) Coupled with ICP-TOFMS for Trace Elemental Analysis of Quartz.
DOI: 10.13130/rd_unimi/pddrps/xr4yt0
title: Marzo_1937_Pagina_001.jpg

description: Bulletin of 1937/03/01
DOI: 10.17632/r9h9xj452x.1
title: Trace elemental analysis of quartz using femtosecond laser ICP-TOFMS

description: Comparison of Two Femtosecond Laser Techniques (Matrix-Array fs-LA and Conventional fs-LA) Coupled with ICP-TOFMS for Trace Elemental Analysis of Quartz.
DOI: 10.13130/rd_unimi/pddrps/rshk0g
title: Marzo_1937_Pagina_002.jpg

description: Bulletin of 1937/03/01
DOI: 10.13130/rd_unimi/pddrps/d8psc8
title: Marzo_1937_Pagina_003.jpg

description: Bulletin of 1937/03/01
DOI: 10.13130/rd_unimi/pddrps/udc8o7
title: Marzo_1937_Pagina_004.jpg

description: Bulletin of 1937/03/01
DOI: 10.13130/rd_unimi/pddrps/2ivupn
title: Marzo_1937_Pagina_005.jpg

description: Bulletin of 1937/03/01
DOI: 10.17632/fhrbygmsm9
title: Design Optimization of Non-Uniform Perforated Solar Screens for Enhanced Daylighting in a Tropical Gymnasium

description: This dataset contains the complete preliminary analysis and optimization results supporting the research article "Design Optimization of Non-Uniform Perforated Solar Screens for Enhanced Daylighting in a Tropical Gymnasium."
The study investigates the performance of non-uniform perforated solar screens (PSS) in a wide-span gymnasium located in Bangkok, Thailand. The dataset covers simulations for both uniform and non-uniform PSS configurations to optimize for Spatial Daylight Autonomy (sDA), Useful Daylight Illumination (UDI), Spatial Disturbing Glare (sDG), Annual Average Uniformity (U_avg), and Temporal Uniformity (U_t).
The data is provided in two formats: XML (Archive) and CSV (for Visualization).

1. CSV Files (for Visualization)
These files are formatted specifically for use with Design Explorer (https://tt-acm.github.io/DesignExplorer/). They are separated by different sections described in the paper.

2.XML File (Archive)
This file contains the raw, aggregated data from all simulation runs. It is intended for archival purposes.

Variable Dictionary (Data Attributes)
The datasets contain the following design variables and performance metrics:
Type: Classification of the screen (Uniform / Non-uniform).
G (E/W/S): Gradient Contrast for East, West, and South façades (Controls minimum perforation size at the bottom).
F (E/W/S): Perforation Sizing Factor for East, West, and South façades (Controls maximum perforation size at the top).
PP (E/W/S): Resulting Perforation Percentage for the respective façade.
PP (avg): Average Perforation Percentage across all façades.
sDA: Spatial Daylight Autonomy
UDI: Useful Daylight Illuminance (100-3000 lux).
sDG: Spatial Disturbing Glare.
E_avg: Average Illuminance Level (lux).
U_avg: Annual Average Uniformity (Average of Minimum Illuminance / Average Illuminance for all analyzed daylit hours).
U_t: Temporal Uniformity (% of Hours that meets Uniformity Ratio of 0.40 and Spatial Useful Daylight Illumination of 75%)
DOI: 10.13130/rd_unimi/pddrps/gr8qjr
title: Marzo_1937_Pagina_007.jpg

description: Bulletin of 1937/03/01
DOI: 10.13130/rd_unimi/pddrps/vafh3x
title: Marzo_1937_Pagina_006.jpg

description: Bulletin of 1937/03/01
DOI: 10.17632/fhrbygmsm9.1
title: Design Optimization of Non-Uniform Perforated Solar Screens for Enhanced Daylighting in a Tropical Gymnasium

description: This dataset contains the complete preliminary analysis and optimization results supporting the research article "Design Optimization of Non-Uniform Perforated Solar Screens for Enhanced Daylighting in a Tropical Gymnasium."
The study investigates the performance of non-uniform perforated solar screens (PSS) in a wide-span gymnasium located in Bangkok, Thailand. The dataset covers simulations for both uniform and non-uniform PSS configurations to optimize for Spatial Daylight Autonomy (sDA), Useful Daylight Illumination (UDI), Spatial Disturbing Glare (sDG), Annual Average Uniformity (U_avg), and Temporal Uniformity (U_t).
The data is provided in two formats: XML (Archive) and CSV (for Visualization).

1. CSV Files (for Visualization)
These files are formatted specifically for use with Design Explorer (https://tt-acm.github.io/DesignExplorer/). They are separated by different sections described in the paper.

2.XML File (Archive)
This file contains the raw, aggregated data from all simulation runs. It is intended for archival purposes.

Variable Dictionary (Data Attributes)
The datasets contain the following design variables and performance metrics:
Type: Classification of the screen (Uniform / Non-uniform).
G (E/W/S): Gradient Contrast for East, West, and South façades (Controls minimum perforation size at the bottom).
F (E/W/S): Perforation Sizing Factor for East, West, and South façades (Controls maximum perforation size at the top).
PP (E/W/S): Resulting Perforation Percentage for the respective façade.
PP (avg): Average Perforation Percentage across all façades.
sDA: Spatial Daylight Autonomy
UDI: Useful Daylight Illuminance (100-3000 lux).
sDG: Spatial Disturbing Glare.
E_avg: Average Illuminance Level (lux).
U_avg: Annual Average Uniformity (Average of Minimum Illuminance / Average Illuminance for all analyzed daylit hours).
U_t: Temporal Uniformity (% of Hours that meets Uniformity Ratio of 0.40 and Spatial Useful Daylight Illumination of 75%)
DOI: 10.13130/rd_unimi/pddrps/j7eatg
title: Marzo_1937_Pagina_008.jpg

description: Bulletin of 1937/03/01
DOI: 10.13130/rd_unimi/pddrps/kjnbqe
title: Marzo_1937_Pagina_009.jpg

description: Bulletin of 1937/03/01
DOI: 10.17632/3kw5zkgrb7
title: Supplement table_IFN-gamma

description: Supplement table for manuscript "The Prognostic Role of Reactive Dermatoses in Anti-Interferon-γ Autoantibody Patients: A Retrospective Cohort Study "
DOI: 10.13130/rd_unimi/pddrps/oewwzp
title: Marzo_1937_Pagina_010.jpg

description: Bulletin of 1937/03/01
DOI: 10.13130/rd_unimi/pddrps/8syneu
title: Marzo_1937_Pagina_011.jpg

description: Bulletin of 1937/03/02
DOI: 10.13130/rd_unimi/pddrps/g3rcla
title: Marzo_1937_Pagina_012.jpg

description: Bulletin of 1937/03/02
DOI: 10.17632/3kw5zkgrb7.2
title: Supplement table_IFN-gamma

description: Supplement table for manuscript "The Prognostic Role of Reactive Dermatoses in Anti-Interferon-γ Autoantibody Patients: A Retrospective Cohort Study "
DOI: 10.13130/rd_unimi/pddrps/zqmnul
title: Marzo_1937_Pagina_013.jpg

description: Bulletin of 1937/03/02
DOI: 10.13130/rd_unimi/pddrps/ee2wmk
title: Marzo_1937_Pagina_014.jpg

description: Bulletin of 1937/03/02
DOI: 10.13130/rd_unimi/pddrps/9zfhkc
title: Marzo_1937_Pagina_015.jpg

description: Bulletin of 1937/03/02
DOI: 10.13130/rd_unimi/pddrps/bpshn2
title: Marzo_1937_Pagina_016.jpg

description: Bulletin of 1937/03/02
DOI: 10.17632/kjnnsxhrbj
title: Cell-free genomics reveals fundamental regulatory principles of the Mycobacterium tuberculosis transcription cycle. Froom et al.

description: CFG_inVitro_transcription_gels.pptx: These data show the radiolabeled transcript output from in vitro transcription reactions performed using purified proteins and linear DNA scaffolds. Figure 4D compares radiolabeled trinucleotide products generated by purified Mycobacterium tuberculosis RNA polymerase in complex with the initiation factor SigA and essential transcription factors (TFs) CarD and RbpA, with and without the essential TF holo-WhiB1 (the holo form of the WhiB1 protein contains a [4Fe-4S]2+ cluster coordinated by four encoded cysteines). Figure 4D demonstrates that adding holo-WhiB1 to the reaction increases the amount of trinucleotide products produced on a linear DNA piece containing the PdesA2 promoter from the M. tuberculosis genome, indicating that holo-WhiB1 activates this promoter. Figures 5D, 5E and 5F compare the influence of the essential M. tuberculosis Nus factors (NusA and NusG) on three different terminators from the M. tuberculosis genome: pitB, icd1, and the 5S rRNA. Comparing the ratiometric intensities of the terminator band versus the "run-off" band (indicative of longer RNA products that were produced by RNA polymerase that did not terminate but processed to the end of the linear DNA fragment) demonstrates that NusG stimulates termination on pitB, while NusA does not; NusA stimulates termination more than NusG on icd1; and NusA and NusG additively stimulate termination on the 5S rRNA terminator. 

whiB1_transcription_assay_realtime_fluorescence_measurements.xlsx: These are the raw measurements of real-time fluorescence traces obtained by adding Mtb RNAP holoenzyme, with and without holo-WhiB1, to a pre-incubation mix of all four ribonucleotides (NTPs) and DNA constructs containing variants of PdesA2 with WT or mutant promoter elements driving the expression of the Spinach-mini RNA aptamer that binds to and alters the emission of the fluorescent dye DFHBI. The fluorescence values measured in a control reaction without NTPs were subtracted from each reaction trace, and the value measured at time zero was also subtracted from all values within a given reaction trace, to obtain the background-subtracted and normalized values. These data demonstrate that a minimal GAT motif and intermediate-strength -35 promoter element are each required for WhiB1 to activate transcription of the desA2 promoter, while converting the intermediate-strength -35 to a strong -35 causes WhiB1 to repress transcription.
DOI: 10.13130/rd_unimi/pddrps/cf37fs
title: Marzo_1937_Pagina_017.jpg

description: Bulletin of 1937/03/02
DOI: 10.13130/rd_unimi/pddrps/u89xxa
title: Marzo_1937_Pagina_018.jpg

description: Bulletin of 1937/03/02