cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20009229.v1
title: Effects of supplementation with Saccharomyces cerevisiae and aerobic training on physical performance of Mangalarga Marchador mares

description: The objective of this study was to assess the effects of supplementation with Saccharomyces cerevisiae on the performance of Mangalarga Marchador equines and to verify the effectiveness of the aerobic training protocol adopted. The study used 14 Mangalarga Marchador mares in a completely randomized split-plot design. The plots were made up of two treatments (groups Probiotic and Control) and the split plots comprised two maximal-effort physical tests applied both before and after the six weeks of training. The animals' spirometric parameters, heart rate, and plasma lactate levels were assessed. No difference was found between the Probiotic and Control groups in any of the parameters assessed. A difference was found in the physical tests for time-to-fatigue, aerobic and anaerobic thresholds, speed at which plasma lactate levels reached 2 and 4 mmol/L, speed at which the heart rate reached 200 bpm, and the lactate level at 30 min of the recovery period. Supplementation with Saccharomyces cerevisiae has no effect on physical conditioning of Mangalarga Marchador mares and the aerobic training protocol adopted increases the animals' aerobic and anaerobic capacity.
DOI: 10.6084/m9.figshare.20009232
title: Productive performance and blood parameters of bulls fed diets containing babassu mesocarp bran and whole or ground corn

description: The objective of the present study was to evaluate the effects of corn milling and the inclusion of babassu mesocarp bran (BMB) on productive performance, digestibility of dietary nutrients, and blood parameters of dairy crossbred (Holstein-Gyr) bulls finished in confinement. Twenty-four bulls were fed four different experimental diets, containing two levels of inclusion of BMB (0 and 41.24%) and corn supplied in two different forms (ground and whole), for 98 days (77 days of data collection and 21 days of adaptation). The intakes and digestibility coefficients of the dry matter (DM) and nutrients were determined. There were no significant interaction effects of the BMB inclusion level and the form of corn used on the performance and digestibility variables. The intakes of DM, crude protein (CP), and neutral detergent fibre (NDF) increased with the inclusion of BMB in the diets. However, the inclusion of BMB in the diets decreased the ether extract intake, the NDF apparent digestibility, and the feed efficiency of DM and CP. Dry matter and NDF intakes also increased with the use of ground corn in the diet, which promoted an increase in the intake of total digestible nutrients (TDN), digestibility of non-fibrous carbohydrates, and average daily gain. However, the supply of ground corn reduced the feed efficiency of TDN. The remaining measured variables did not vary with the tested diets. The levels of plasma protein and albumin remained normal, but glucose concentrations were always high, irrespective of the tested diet. The form of corn supplied and the level of BMB inclusion had a significant interaction effect on the levels of triglycerides, urea, aspartate aminotransferase, and alkaline phosphatase. Babassu mesocarp bran can be included up to 41.24% in the diet of confined bulls without a negative effect on the animal weight gain. Corn should be supplied ground because this form improves the performance of crossbred bulls
DOI: 10.6084/m9.figshare.20009232.v1
title: Productive performance and blood parameters of bulls fed diets containing babassu mesocarp bran and whole or ground corn

description: The objective of the present study was to evaluate the effects of corn milling and the inclusion of babassu mesocarp bran (BMB) on productive performance, digestibility of dietary nutrients, and blood parameters of dairy crossbred (Holstein-Gyr) bulls finished in confinement. Twenty-four bulls were fed four different experimental diets, containing two levels of inclusion of BMB (0 and 41.24%) and corn supplied in two different forms (ground and whole), for 98 days (77 days of data collection and 21 days of adaptation). The intakes and digestibility coefficients of the dry matter (DM) and nutrients were determined. There were no significant interaction effects of the BMB inclusion level and the form of corn used on the performance and digestibility variables. The intakes of DM, crude protein (CP), and neutral detergent fibre (NDF) increased with the inclusion of BMB in the diets. However, the inclusion of BMB in the diets decreased the ether extract intake, the NDF apparent digestibility, and the feed efficiency of DM and CP. Dry matter and NDF intakes also increased with the use of ground corn in the diet, which promoted an increase in the intake of total digestible nutrients (TDN), digestibility of non-fibrous carbohydrates, and average daily gain. However, the supply of ground corn reduced the feed efficiency of TDN. The remaining measured variables did not vary with the tested diets. The levels of plasma protein and albumin remained normal, but glucose concentrations were always high, irrespective of the tested diet. The form of corn supplied and the level of BMB inclusion had a significant interaction effect on the levels of triglycerides, urea, aspartate aminotransferase, and alkaline phosphatase. Babassu mesocarp bran can be included up to 41.24% in the diet of confined bulls without a negative effect on the animal weight gain. Corn should be supplied ground because this form improves the performance of crossbred bulls
DOI: 10.6084/m9.figshare.20009235
title: Soybean in different forms of processing in the feeding of crossbred cows on brachiaria grass pastures

description: The objective was to evaluate intake and digestibility of nutrients, as well as milk production and composition of the milk from F1 Holstein × Gyr cows kept on pasture, supplemented with sugarcane and concentrate (28% CP). Five cows with 150±14 lactation days and average milk production of 7.1±2.1 kg/day were distributed in a 5 × 5 Latin square design. The treatments were: soybean meal-based concentrate; soy bean-based concentrate; ground soybean-based concentrate; roasted soybean-based concentrate; and ground, roasted soybean-based concentrate. Dry matter (DM), organic matter, crude protein (CP), neutral detergent fiber (NDF), total carbohydrates and total digestible nutrients intakes were not affected by the diet, but ether extract intake was higher for the animals fed soy bean-based diets than those fed soybean meal. The digestibility of DM, NDF and CP did not differ. The corrected milk yield differed between treatments; animals on the treatment with soy bean-based concentrate had the lowest production in relation to the others, which did not differ from each other. There was no difference between treatments for milk composition. Thus, soybean meal can be replaced by ground soy beans or roasted soy beans (ground or whole) in diets for low-yield cows reared on Brachiaria decumbens pastures with no harm to milkproduction or composition. Therefore, the use of these alternative raw materials is recommended whenever their inclusion represents lower feeding costs.
DOI: 10.6084/m9.figshare.20009235.v1
title: Soybean in different forms of processing in the feeding of crossbred cows on brachiaria grass pastures

description: The objective was to evaluate intake and digestibility of nutrients, as well as milk production and composition of the milk from F1 Holstein × Gyr cows kept on pasture, supplemented with sugarcane and concentrate (28% CP). Five cows with 150±14 lactation days and average milk production of 7.1±2.1 kg/day were distributed in a 5 × 5 Latin square design. The treatments were: soybean meal-based concentrate; soy bean-based concentrate; ground soybean-based concentrate; roasted soybean-based concentrate; and ground, roasted soybean-based concentrate. Dry matter (DM), organic matter, crude protein (CP), neutral detergent fiber (NDF), total carbohydrates and total digestible nutrients intakes were not affected by the diet, but ether extract intake was higher for the animals fed soy bean-based diets than those fed soybean meal. The digestibility of DM, NDF and CP did not differ. The corrected milk yield differed between treatments; animals on the treatment with soy bean-based concentrate had the lowest production in relation to the others, which did not differ from each other. There was no difference between treatments for milk composition. Thus, soybean meal can be replaced by ground soy beans or roasted soy beans (ground or whole) in diets for low-yield cows reared on Brachiaria decumbens pastures with no harm to milkproduction or composition. Therefore, the use of these alternative raw materials is recommended whenever their inclusion represents lower feeding costs.
DOI: 10.6084/m9.figshare.20009238
title: Characteristics of the carcass of goats of different genotypes fed pineapple (Ananas comosus L.) stubble hay

description: The effect of the substitution of Tifton hay (0, 33, 66, 100 g/100 g) for pineapple (Ananas comosus L.) stubble hay was evaluated in the characteristics of the carcasses of goats of an unknown breed (UB) in feedlot. Thirty-two bucks with an average initial live weight of 17.5±1.3 kg, at approximately 150 days of age, were housed in individual stalls provided with feeding and drinking troughs. The animals were distributed in a completely randomized design with four treatments and eight replications. There was a quadratic effect on the weights of hot carcass and cold carcass, empty body, and loin eye area. A linear increase was observed for losses by carcass cooling. The weights of commercial cuts and the weights of total muscle, total bone, intramuscular fat, and total fat decreased linearly. All morphometric measurements were influenced by the inclusion of pineapple stubble hay in the .diets. Substitution of Tifton hay for pineapple stubble hay at the level of 33 g/100 g improves the carcasses of UB goats qualitatively and quantitatively
DOI: 10.6084/m9.figshare.20009238.v1
title: Characteristics of the carcass of goats of different genotypes fed pineapple (Ananas comosus L.) stubble hay

description: The effect of the substitution of Tifton hay (0, 33, 66, 100 g/100 g) for pineapple (Ananas comosus L.) stubble hay was evaluated in the characteristics of the carcasses of goats of an unknown breed (UB) in feedlot. Thirty-two bucks with an average initial live weight of 17.5±1.3 kg, at approximately 150 days of age, were housed in individual stalls provided with feeding and drinking troughs. The animals were distributed in a completely randomized design with four treatments and eight replications. There was a quadratic effect on the weights of hot carcass and cold carcass, empty body, and loin eye area. A linear increase was observed for losses by carcass cooling. The weights of commercial cuts and the weights of total muscle, total bone, intramuscular fat, and total fat decreased linearly. All morphometric measurements were influenced by the inclusion of pineapple stubble hay in the .diets. Substitution of Tifton hay for pineapple stubble hay at the level of 33 g/100 g improves the carcasses of UB goats qualitatively and quantitatively
DOI: 10.6084/m9.figshare.20009239
title: Seasonal and circadian variation of the sexual behavior of Morada Nova rams in tropical environment

description: The objective of this study was to evaluate the seasonal and daily variation in sexual behavior of Morada Nova breeders raised in the Brazilian semi-arid region. The data were collected from four rams and 114 ewes of the Morada Nova breed - 55 females in the dry season and another 59 in the rainy season. Observations were performed in the dry and rainy seasons, divided into four times of the day: morning (05:01 h to 11.00 h), afternoon (11.01 h to 17.00 h), evening (17.01 h to 23:00 h), and dawn (23:01 h to 05.00 h). Sexual behavior was assessed for a period of 24 h/day, in a breeding season. One ram was used each three days. The behavioral patterns of the rams in view of females were observed during the recognition (sniffing the female urine, bouts of anogenital sniffing, and flehmen reaction), preparatory (exposure of tongue and penis, leg-kicking, and low-pitched bleats), and copulatory (number of mounts, number of ejaculations, and refractory period) phases. The rams were more active during daytime. The animals in the rainy season showed more efficient sexual behaviors by the lower frequency of mounts per ejaculation and greater number of ejaculations in a shorter time. In contrast, courting behaviors such as exposure of tongue, number of mounts, low-pitched bleats, and leg-kicking were more frequent in the dry season. In the semi-arid region with latitudes close to 7°N, Morada Nova rams have a higher intensity of precopulatory behaviors and lower frequency of ejaculations in the dry season.
DOI: 10.6084/m9.figshare.20009239.v1
title: Seasonal and circadian variation of the sexual behavior of Morada Nova rams in tropical environment

description: The objective of this study was to evaluate the seasonal and daily variation in sexual behavior of Morada Nova breeders raised in the Brazilian semi-arid region. The data were collected from four rams and 114 ewes of the Morada Nova breed - 55 females in the dry season and another 59 in the rainy season. Observations were performed in the dry and rainy seasons, divided into four times of the day: morning (05:01 h to 11.00 h), afternoon (11.01 h to 17.00 h), evening (17.01 h to 23:00 h), and dawn (23:01 h to 05.00 h). Sexual behavior was assessed for a period of 24 h/day, in a breeding season. One ram was used each three days. The behavioral patterns of the rams in view of females were observed during the recognition (sniffing the female urine, bouts of anogenital sniffing, and flehmen reaction), preparatory (exposure of tongue and penis, leg-kicking, and low-pitched bleats), and copulatory (number of mounts, number of ejaculations, and refractory period) phases. The rams were more active during daytime. The animals in the rainy season showed more efficient sexual behaviors by the lower frequency of mounts per ejaculation and greater number of ejaculations in a shorter time. In contrast, courting behaviors such as exposure of tongue, number of mounts, low-pitched bleats, and leg-kicking were more frequent in the dry season. In the semi-arid region with latitudes close to 7°N, Morada Nova rams have a higher intensity of precopulatory behaviors and lower frequency of ejaculations in the dry season.
DOI: 10.6084/m9.figshare.20009241
title: Different true-protein sources do not modify the metabolism of crossbred Bos taurus × Bos indicus growing heifers

description: The present study was conducted to investigate the effect of alternative true-protein sources to soybean meal, with different ruminal degradability, using a sugarcane-based diet, on nutrient digestion, ruminal fermentation, efficiency of microbial protein synthesis and passage rate in prepubertal dairy heifers. Eight crossbred rumen- and duodenum-cannulated Holstein × Gyr dairy heifers (202.0±11.5 kg BW) were evaluated in a 4 × 4 Latin square experimental design with four treatments and four periods in two simultaneous replicates. Dietary treatments were: soybean meal; cottonseed meal; peanut meal; and sunflower meal. When associated with diets containing sugarcane, the different protein sources did not affect intake or digestibility of dry mater, crude protein, organic matter and neutral detergent fiber. The average ruminal pH, NH3-N and concentration of total volatile fatty acids were not different among the diets supplied. The concentration of butyric acid was different among the protein sources, wherein the animals fed the diet with sunflower meal presented lower values than those fed the other sources. Diets did not affect nitrogen balance, microbial nitrogen, microbial synthesisefficiency, estimated dry matter flow, or passage rate. Alternative protein sources can be used to reduce the costs without changing the animal metabolism.
DOI: 10.6084/m9.figshare.22605698.v1
title: Additional file 6 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 6: Table S2. Marker genes / differentially expressed genes_cell type level 1. (Table 2.1) Overview of marker genes and references used to annotate and specify the respective clusters. (Table 2.2–2.10) Depicted are the differentially expressed genes in each cluster compared to the remaining clusters of the cell type level 1 (marker genes). Listed for each gene is the Log fold change (log2-fold change), the p-value und the adjusted p-value (false discovery rate). We used the Welch’s t-test (cellxgene or diffxpy) for the discovery of differentially expressed genes between two groups of cells. A minimum of 10 cells in each group was required for calculation.
DOI: 10.6084/m9.figshare.22605707
title: Additional file 8 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 8: Table S4. Marker genes / Differential expressed genes_ cluster cell type level 2. (Table 4.1) Overview of marker genes and references used to annotate and specify the respective clusters. (Table 4.2–4.30) Depicted are the differential expressed genes in each cluster compared to the remaining clusters of the cell type level 2 (marker genes). Listed for each gene is the Log fold change (log2-fold change), the p-value und the adjusted p-value (false discovery rate). We used the Welch’s t-test (cellxgene or diffxpy) for the discovery of differentially expressed genes between two groups of cells. A minimum of 10 cells in each group was required for calculation.
DOI: 10.6084/m9.figshare.22605707.v1
title: Additional file 8 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 8: Table S4. Marker genes / Differential expressed genes_ cluster cell type level 2. (Table 4.1) Overview of marker genes and references used to annotate and specify the respective clusters. (Table 4.2–4.30) Depicted are the differential expressed genes in each cluster compared to the remaining clusters of the cell type level 2 (marker genes). Listed for each gene is the Log fold change (log2-fold change), the p-value und the adjusted p-value (false discovery rate). We used the Welch’s t-test (cellxgene or diffxpy) for the discovery of differentially expressed genes between two groups of cells. A minimum of 10 cells in each group was required for calculation.
DOI: 10.6084/m9.figshare.22605710.v1
title: Additional file 9 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 9: Table S5. DE genes / Differential expressed genes MS vs Control cell type level 2. Depicted are the differential expressed genes in MS compared to control (Ctrl) for each cluster of cell type level 1 (DE genes). Listed for each gene is the Log fold change (log2-fold change), the p-value und the adjusted p-value (false discovery rate). We used the Welch’s t-test (cellxgene or diffxpy) for the discovery of differentially expressed genes between two groups of cells. A minimum of 10 cells in each group was required for calculation.
DOI: 10.6084/m9.figshare.22605695
title: Additional file 5 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 5: Table S1. Subject demographics and technical information on scRNA-Seq. (Table 1.1) Composition of the three datasets, subject demographics of included multiple sclerosis (MS, n = 20) and control (IIH/HC = Ctrl, n = 11) patients, MRI features and CSF findings are listed. For dataset 3, non-included samples from Ramesh et al. [13] are listed (grayed out) and their exclusion criteria are shown. See methods for the primary source of the three datasets. Column legend: IIH = idiopathic intracranial hypertension, MS = multiple sclerosis, CIS = clinically isolated syndrome, f = female, m = male, rel_gd = relapse, lesions with gadolinium enhancement detected on magnetic resonance imaging (MRI), rel_nogd = relapse, no lesion with gadolinium enhancement on MRI, MS-typical MRI = MS-typical lesion pattern in characteristic locations (ovoid periventricular lesions/Dawson fingers, cortical or juxtacortical, infratentorial and spinal cord) (1) / no Ms-typical lesion pattern (0), active = active relapse, Gd +  = gadolinium detected (1) or not detected (0) in MRI lesion, months from onset = months from first symptoms until sample collection, cell = cell count/µL obtained via lumbar puncture, lympho = lymphocytes/µL of cerebrospinal fluid (CSF), granulo = granulocytes/µL of CSF, rbc = red blood cell count/µL CSF, protein = obtained protein concentration in mg/L CSF, lactate = CSF lactate in nmol/L, glucose = CSF glucose in mg/dL, OCB +  = any oligoclonal bands (OCB) detected in CSF (1) or not detectable (0). Barrier disruption = no barrier disruption (0) or present barrier disruption (1). CSF index = derived from the Reiber scheme [54] (IgG CSF/IgG serum index compared to the albumin CSF/albumin serum index), no barrier disruption (none), intrathecal immunoglobulin synthesis (Ig only), intrathecal immunoglobulin synthesis plus barrier disruption (barrier_and_Ig). IgG-index &gt; 0.7 = IgG synthesis in the CSF. All parameters were obtained within 24 h of CSF sampling. (Table 1.2) Technical information on scRNA-seq results of all patients (MS, n = 20) and healthy controls (Ctrl, n = 11) included in the study are listed. The sequencing technique for generation of the 10 × libraries is listed for the different datasets (10 × Genomics scRNA-seq kit version). Furthermore, the total number of measured cells after sequencing and genome alignment (total cells per sample) and the average number of detected genes per cells (average gene per cell) used for downstream analysis is depicted.
DOI: 10.6084/m9.figshare.22605704.v1
title: Additional file 7 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 7: Table S3. DE genes / Differentially expressed genes MS vs control cell type level 1. Depicted are the differential expressed genes in MS compared to control (Ctrl) for each cluster of cell type level 1 (DE genes). Listed for each gene is the Log fold change (log2-fold change), the p-value und the adjusted p-value (false discovery rate). We used the Welch’s t-test (cellxgene or diffxpy) for the discovery of differentially expressed genes between two groups of cells. A minimum of 10 cells in each group was required for calculation.
DOI: 10.6084/m9.figshare.22605698
title: Additional file 6 of Integrated single-cell transcriptomics of cerebrospinal fluid cells in treatment-naïve multiple sclerosis

description: Additional file 6: Table S2. Marker genes / differentially expressed genes_cell type level 1. (Table 2.1) Overview of marker genes and references used to annotate and specify the respective clusters. (Table 2.2–2.10) Depicted are the differentially expressed genes in each cluster compared to the remaining clusters of the cell type level 1 (marker genes). Listed for each gene is the Log fold change (log2-fold change), the p-value und the adjusted p-value (false discovery rate). We used the Welch’s t-test (cellxgene or diffxpy) for the discovery of differentially expressed genes between two groups of cells. A minimum of 10 cells in each group was required for calculation.