DOI: 10.5061/dryad.gxd2547xf
title: Data from: Long-term social memory of mate copying in Drosophila melanogaster is localized in the mushroom bodies

description: Long-term social memory (LTSM) is a key feature to elicit the cultural
 inheritance of behaviour independently of genetics. However, the
 neurobiological basis of LTSM remains largely unknown. We previously used
 the Drosophila animal model, which is known to perform mate copying
 through observational learning of the mate choice of conspecifics to show
 that the expression of the rutabaga gene, a
 calcium/calmodulin-dependent adenylyl cyclase (AC-Rut+) that acts as a
 coincidence detector enabling associative learning, is necessary and
 sufficient in the γ-Kenyon cells (KCs) of the mushroom bodies (MBs). Here,
 we show that the expression of AC-Rut+ in both the g- and the
 a/b-KCs is required for LTSM involving de novo protein synthesis in a
 mate-copying context, whether using demonstrations involving real flies or
 involving pictures of copulating conspecifics. Thus, pathways of short-
 and long-term memory show considerable overlap in the MBs across social vs
 asocial learning contexts.
description: We did behavioral observations of fruit fly mate choice decisions
 using different observer female genotypes to identify the formation of
 long-term memory. 
description: # Data from: “Long-term social memory of mate copying in Drosophila
 melanogaster is localized in the mushroom bodies”
 [https://doi.org/10.5061/dryad.gxd2547xf](https://doi.org/10.5061/dryad.gxd2547xf) ## Description of the data and file structure Data from: “Long-term social memory of mate copying in Drosophila melanogaster is localized in the mushroom bodies” by Sabine Nöbel, Etienne Danchin & Guillaume Isabel Contacts Sabine Nöbel [sabine.noebel@zoologie.uni-halle.de](mailto:sabine.noebel@zoologie.uni-halle.de) Etienne Danchin [etienne.danchin@univ-tlse3.fr](mailto:etienne.danchin@univ-tlse3.fr) Guillaume Isabel [guillaume.isabel@univ-tlse3.fr](mailto:guillaume.isabel@univ-tlse3.fr) ### Files and variables #### File: Data\_RutLTM.xlsx **Description:**  To study the formation of long-term social memory in Drosophila melanogaster, we used a training protocol with 5 repeated demonstrations. To identify the neurons involved, we used different Gal4 driver lines specific for MB neurons and rutabaga mutants that show a learning deficit in the absence of rutabaga protein. By specifically expressing the protein in certain groups of neurons, we were able to determine which neurons are essential for the formation of long-term social memory. ##### Variables: | Variable name | Levels | Explanation | | :------------ | :--------------- | :----------------------------------------------------------------------------------- | | dateD | | Day of the demonstration | | timeD | | Time of the demonstration | | demoType | Live | Demonstration with living flies | | | Foto | Demonstration with pictures | | PicID | | Picture identification number | | block | | Indicates which trials were performed at the same time | | treatment | | Genotype of the observer female | | | 7 | rut/rut; C739/+ | | | 8 | rut/rut; C739/UAS-rut+ | | | 9 | rut/rut; NP1131/+ | | | 10 | rut/rut; NP1131/UAS-rut+ | | | 11 |  +/+; C739/+ | | | 12 |  +/+; NP1131/+ | | | 16 | rut/rut;; MB247/+  | | | 17 | rut/rut; UAS-rut/+; MB247/+ | | | 18 |  +/+;; MB247/+ | | | 19 | Wild type (WT +/+) fed with cycloheximide (CXM) | | | 20 | Wild type (WT +/+) | | neurons | alpha/beta | Group of neurons addressed in the Mushroom bodies | | | gamma | | | | alpha/beta+gamma | | | temp | | Room temperature \[°C] | | humidity | | Humidity in the room \[%] | | AP | | Air pressure \[hPa] | | ColChosenD | | Male color copulating in the demonstration | | | G | Green  | | | P | Pink | | dateT | | Day of the test | | timeT | | Time of the test | | ColCourt1 | | Male color that started the 1. courtship | | | G | Green  | | | P | Pink | | ColCourt2 | | Male color that started the 2. courtship | | | G | Green  | | | P | Pink | | ColChosenT | | Male color copulating in the test | | | G | Green  | | | P | Pink | | MCS | | Mate-copying scores | | | 0 | Observer female chose to copulate with the opposite color as the demonstrator female | | | 1 | Observer female chose to copulate with the same color as the demonstrator female |