DOI: 10.5061/dryad.9ghx3ffxd
title: Electrophysiology and anatomy data for: Projection-specific integration of convergent thalamic and retrosplenial signals in the presubicular head direction cortex

description: Head-direction (HD) signals function as the brain’s internal compass. They
 are organized as an attractor and anchor to the environment via visual
 landmarks. Here, we examine how thalamic HD signals and visual information
 from the retrosplenial cortex combine in the presubiculum. We find that
 monosynaptic excitatory connections from the anterior thalamic nucleus and
 from retrosplenial cortex converge on single-layer 3 pyramidal neurons in
 the dorsal portion of the mouse presubiculum. Independent dual wavelength
 photostimulation of these inputs in slices leads to action potential
 generation preferentially for near-coincident inputs, indicating that
 layer 3 neurons can transmit a visually matched HD signal to the medial
 entorhinal cortex. Layer 4 neurons, which innervate the lateral mammillary
 nucleus, form a second step in the association of HD and landmark signals.
 They receive little direct input from thalamic and retrosplenial axons. We
 show that layer 4 cells are excited di-synaptically, transforming regular
 spiking activity into bursts of action potentials, and that their firing
 is enhanced by cholinergic agonists. Thus, a coherent sense of orientation
 involves projection-specific translaminar processing in the presubiculum,
 where neuromodulation facilitates landmark updating of HD signals in the
 lateral mammillary nucleus.
description: # Electrophysiology and anatomy data for: Projection-specific integration
 of convergent thalamic and retrosplenial signals in the presubicular head
 direction cortex Dataset DOI:
 [10.5061/dryad.9ghx3ffxd](https://doi.org/10.5061/dryad.9ghx3ffxd) ##
 Description of the data and file structure Here are available
 electrophysiology data for the following research article:
 "Projection-specific integration of convergent thalamic and
 retrosplenial signals in the presubicular head direction cortex"
 ([https://doi.org/10.7554/eLife.92443.2](https://doi.org/10.7554/eLife.92443.2)) Data are provided in their original format (.abf) and can be opened using AxoGraph or with code language (Matlab, Python, etc...), using adequate script. Data are organized by figures and some figures use the same data set. For each figure, a unique .csv file summarizes the analysis for this figure, with a sheet for each figure panel. Each .zip file of this dataset contains the data associated with a specific figure from the publication. It also contains a summary table file detailing the organization of the data in this folder, with the main results values shown in the figures. Each folder is divided by figure panels and then by cell type (when applicable, ex: ATN- or RSC-receiving, layer 3 or 4). Electrophysiology (whole-cell patch-clamp) data are in .abf (axon binary file) format and named as follows: \.abf where YYYY represents a year (ex: 2018), M a month (ex: January 1 to September 9, October o, November n, December d), DD a day (ex: 17), a recording ID in the day. For example: 18417011.abf was recorded on April 17th 2018. Each .abf cell file contains the current command and potential traces (IN0/IN1, either in V-Clamp or I-Clamp) and stimulation (LED) command (IN11 and/or IN12) for multiple trials. Metadata, current or LED stimulation command values (amplitude, duration) are directly available in the file. Additionally, we provide a spreadsheet named ABF_files_info.xlsx detailing the channel names and number of sweep/trials for each .abf file in the dataset. The .abf files can be viewed in the [pClamp Software Suite](https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite) from by Molecular Devices or in Python ([pyABF library)](https://github.com/swharden/pyABF) or in Matlab ([https://github.com/fcollman/abfload/tree/master](https://github.com/fcollman/abfload/tree/master)). ### Files and variables #### File: Figure_1.zip **Description:** A table summarizing the image analysis for panels I and J of figure 1, quantifying fluorescence in the presubiculum across layers (I) and doors-ventral slices (J). Values are imported from image analysis in Fiji. For panel I: rows correspond to values of normalized fluorescence along the cortical depth (1200 px) for n = 8 slices each (columns), with mean and SEM. For panel J: Quantification of fluorescence inside (layer 3) and outside of PrS, for different dorso-ventral levels in n = 3 animals each. #### File: Figure_2_with_Supplements.zip **Description:** This file regroups .abf files of cells contained in Figure 2 and its supplements. Data are in designated ATN and RSC subfolders, themselves in a root folder for each figure panel. A summary table file indicates the cells and associated .abf (Axon Binary File) files included in each panel, with the values resulting from the different analysis.  #### File: Figure_3_with_Supplement.zip **Description:** This file contains tables and images relative to Panel H for Figure 3 (IMARIS analysis), and .abf files for Figure 3 supplement 1. Cell files are build similarly to Figure 2. The IMARIS analysis generates 3 table files for the position of green spots and red spots for each cell analyzed. These files indicate the X, Y and Z positions of each spot and of the soma, enabling the calculation of the distance to the soma. In F3S1, cell files are in subfolders corresponding to two experimental conditions: Chronos or Crimson stimulations. #### File: Figure_4.zip **Description:** This file contains .abf cell files for Figure 4, organized by panels. Cell files are build similarly to Figure 2. Responses to ATN, RSC or dual stimulations are located in the same file. #### File: Figure_5.zip **Description:** This file contains .abf cell files for Figure 5. Cell files are build similarly to Figure 2. #### File: Figure_6_with_Supplements.zip **Description:** This file contains .abf cell files for Figure 6 and supplement 2, organized by panels. Cell files are build similarly to Figure 2. Cell files are in subfolders corresponding to two experimental conditions: ATN to Layer 4, and RSC to Layer 4 (Fig. 6H), or Layer 3 vs Layer 4 (Fig. 6 Supp. 2). #### File: Figure_7.zip **Description:** This file contains .abf cell files for Figure 7 and supplement 1. Cell files are build similarly to Figure 2.  In Fig 7C, cells are grouped together (detail in attached table file). In Fig 7G, each cell has its own folder containing different files (see attached table for details). ## Code/software All electrophysiology recordings were made using pClamp and all analysis were performed with Axograph X. It is though possible to analyze using other proprietary (Matlab) or open source (Python) softwares. General group analysis and statistical testing were performed with Microsoft Excel and GraphPad Prism.