cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20038518
title: Stability of potato clones resistant to potato virus Y under subtropical conditions

description: Abstract Brazilian potato production is based on cultivars originating from the USA and Europe; however, these cultivars were developed under different environmental conditions than those found in Brazil. The aim of this study was to evaluate the adaptability and stability of 18 potato clones (MLG), developed by the Universidade Federal de Lavras, and four cultivars in six environments in the state of Minas Gerais, Brazil. The following traits were evaluated: marketable tuber yield (MTY), specific gravity (SG), and tuber appearance (TA). Clones, environments, and the genotype × environment interaction had significant effects on all traits. The GGE-biplot explained 71.78, 98.13, and 62.86 % of the sum of squares from the G+G×E for MTY, SG, and TA, respectively. Three MLG clones had higher adaptability and stability, as well as better performance, than the cultivars, making them good candidates for release as cultivars.
DOI: 10.6084/m9.figshare.20038518.v1
title: Stability of potato clones resistant to potato virus Y under subtropical conditions

description: Abstract Brazilian potato production is based on cultivars originating from the USA and Europe; however, these cultivars were developed under different environmental conditions than those found in Brazil. The aim of this study was to evaluate the adaptability and stability of 18 potato clones (MLG), developed by the Universidade Federal de Lavras, and four cultivars in six environments in the state of Minas Gerais, Brazil. The following traits were evaluated: marketable tuber yield (MTY), specific gravity (SG), and tuber appearance (TA). Clones, environments, and the genotype × environment interaction had significant effects on all traits. The GGE-biplot explained 71.78, 98.13, and 62.86 % of the sum of squares from the G+G×E for MTY, SG, and TA, respectively. Three MLG clones had higher adaptability and stability, as well as better performance, than the cultivars, making them good candidates for release as cultivars.
DOI: 10.6084/m9.figshare.20038521
title: Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

description: Abstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably.
DOI: 10.6084/m9.figshare.20038521.v1
title: Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

description: Abstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably.
DOI: 10.6084/m9.figshare.20038522
title: Environmental stratification and genotype recommendation toward the soybean ideotype: a Bayesian approach

description: Abstract The genotype × environment (G×E) interaction plays an essential role in phenotypic expression and can lead to difficulties in genotypes recommendation. Thus, the objectives of this study were: i) propose the Multi-Environment Index Based on Factor Analysis and Ideotype-Design/Markov Chain Monte Carlo (FAI/MCMC index), and ii) apply it for soybean genotypes recommendation. To this end, a data set with 30 soybean genotypes evaluated in 10 environments for grain yield trait was used. Variance components, genetic parameters and genetic values were estimated through MCMC algorithm. Environmental stratification was conducted by factor analyses and the selection of soybean genotypes was performed using the FAI/MCMC index. The results indicated the existence of genotypic variability and G×E interaction. The environments were grouped into three factors. The predicted genetic gains from indirect selection was 4.81%. Thus, our results suggest that the FAI/MCMC index can be successfully used in soybean breeding.
DOI: 10.6084/m9.figshare.20038522.v1
title: Environmental stratification and genotype recommendation toward the soybean ideotype: a Bayesian approach

description: Abstract The genotype × environment (G×E) interaction plays an essential role in phenotypic expression and can lead to difficulties in genotypes recommendation. Thus, the objectives of this study were: i) propose the Multi-Environment Index Based on Factor Analysis and Ideotype-Design/Markov Chain Monte Carlo (FAI/MCMC index), and ii) apply it for soybean genotypes recommendation. To this end, a data set with 30 soybean genotypes evaluated in 10 environments for grain yield trait was used. Variance components, genetic parameters and genetic values were estimated through MCMC algorithm. Environmental stratification was conducted by factor analyses and the selection of soybean genotypes was performed using the FAI/MCMC index. The results indicated the existence of genotypic variability and G×E interaction. The environments were grouped into three factors. The predicted genetic gains from indirect selection was 4.81%. Thus, our results suggest that the FAI/MCMC index can be successfully used in soybean breeding.
DOI: 10.6084/m9.figshare.20038525
title: Evaluation of families derived from backcrosses of processed tomato with dwarfism gene

description: Abstract In comparison to tomato plants without the dwarfism gene, dwarf tomato plants were shorter, had a smaller canopy diameter and remained upright during maturation, thereby reducing the fruit-soil contact, resulting in healthier fruits. This study evaluated tomato families of a BC1F3 population carrying the dwarf gene for industrial yield. The 150 BC1F3 families and the two parents (hybrid H-9889 and accession BGH-9889) as controls were assessed in an augmented block design with four replications. The data were subjected to variance analysis and the F test. A selection index, based on the sum of ranks of Mulamba and Mock (1978), was used for the selection of genotypes, considering the most relevant traits, i.e., canopy diameter, yield, lodging and fruit firmness. Thus, selection resulted in a yield gain of 8.04%.
DOI: 10.6084/m9.figshare.20038525.v1
title: Evaluation of families derived from backcrosses of processed tomato with dwarfism gene

description: Abstract In comparison to tomato plants without the dwarfism gene, dwarf tomato plants were shorter, had a smaller canopy diameter and remained upright during maturation, thereby reducing the fruit-soil contact, resulting in healthier fruits. This study evaluated tomato families of a BC1F3 population carrying the dwarf gene for industrial yield. The 150 BC1F3 families and the two parents (hybrid H-9889 and accession BGH-9889) as controls were assessed in an augmented block design with four replications. The data were subjected to variance analysis and the F test. A selection index, based on the sum of ranks of Mulamba and Mock (1978), was used for the selection of genotypes, considering the most relevant traits, i.e., canopy diameter, yield, lodging and fruit firmness. Thus, selection resulted in a yield gain of 8.04%.
DOI: 10.6084/m9.figshare.20038527
title: SNP genotyping for fast and consistent clustering of maize inbred lines into heterotic groups

description: Abstract Advances in genotyping technologies have transformed the way breeding programs manage their genetic resources. The identification of single nucleotide polymorphisms (SNPs) can improve understanding of the genetic diversity of maize (Zea mays) inbred lines and their classification into heterotic groups, which is useful in determining certain crosses to obtain hybrids with higher yield performance. The genetic diversity of 293 inbred lines was investigated with 5252 SNPs with minor allele frequency (MAF)&gt;5%. There was an average of 525 SNPs per chromosome. Polymorphism information content (PIC) averaged 0.297. The unweighted pair group method with arithmetic mean analysis (UPGMA) and principal component analysis (PCA) based on the genetic distance matrix revealed four similar clusters and high cophenetic correlation coefficients (0.953 and 0.863, respectively). The results showed consistency between genetic distance-based grouping and the heterotic groups previously established using pedigree and topcross information for the inbred lines studied.
DOI: 10.6084/m9.figshare.20038527.v1
title: SNP genotyping for fast and consistent clustering of maize inbred lines into heterotic groups

description: Abstract Advances in genotyping technologies have transformed the way breeding programs manage their genetic resources. The identification of single nucleotide polymorphisms (SNPs) can improve understanding of the genetic diversity of maize (Zea mays) inbred lines and their classification into heterotic groups, which is useful in determining certain crosses to obtain hybrids with higher yield performance. The genetic diversity of 293 inbred lines was investigated with 5252 SNPs with minor allele frequency (MAF)&gt;5%. There was an average of 525 SNPs per chromosome. Polymorphism information content (PIC) averaged 0.297. The unweighted pair group method with arithmetic mean analysis (UPGMA) and principal component analysis (PCA) based on the genetic distance matrix revealed four similar clusters and high cophenetic correlation coefficients (0.953 and 0.863, respectively). The results showed consistency between genetic distance-based grouping and the heterotic groups previously established using pedigree and topcross information for the inbred lines studied.
DOI: 10.6084/m9.figshare.20038528
title: Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

description: Abstract: Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.
DOI: 10.6084/m9.figshare.20038528.v1
title: Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

description: Abstract: Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.
DOI: 10.6084/m9.figshare.20038530
title: Genetic parameters and path analysis for root yield of cassava under drought and early harvest

description: Abstract The objectives of this study were to estimate genetic parameters and identify traits that contribute to early yield in Manihot esculenta Crantz. Ten traits were evaluated in two experiments, one with 138 genotypes and the other with 133, using a completely randomized block design with four replications. All genotype effects were significant. The accuracy was high for the traits, except leaf retention (0.57), and reliability ranged from 0.33 to 0.75. Root weight (RW) and number of roots (NR) showed the highest selection effectiveness (CV r ), although the estimate was less than 1. Moreover, RW and NR obtained high genetic correlation (0.70). Path analysis showed that only number of roots had a significant direct effect on root weight (0.49), and no indirect effect was found. Therefore, accessions exhibited sufficient genetic variability, and indirect selection based on early evaluation of number of roots can be applied in cassava breeding for fresh root yield, increasing gain over time.
DOI: 10.6084/m9.figshare.20038530.v1
title: Genetic parameters and path analysis for root yield of cassava under drought and early harvest

description: Abstract The objectives of this study were to estimate genetic parameters and identify traits that contribute to early yield in Manihot esculenta Crantz. Ten traits were evaluated in two experiments, one with 138 genotypes and the other with 133, using a completely randomized block design with four replications. All genotype effects were significant. The accuracy was high for the traits, except leaf retention (0.57), and reliability ranged from 0.33 to 0.75. Root weight (RW) and number of roots (NR) showed the highest selection effectiveness (CV r ), although the estimate was less than 1. Moreover, RW and NR obtained high genetic correlation (0.70). Path analysis showed that only number of roots had a significant direct effect on root weight (0.49), and no indirect effect was found. Therefore, accessions exhibited sufficient genetic variability, and indirect selection based on early evaluation of number of roots can be applied in cassava breeding for fresh root yield, increasing gain over time.
DOI: 10.6084/m9.figshare.20038531.v1
title: Pollen morphological analysis of papaya (Carica papaya L.)

description: Abstract There is little information on pollen morphological analysis of papaya cultivars with different flesh color and origins. We observed pollen morphological characters of 17 papaya cultivars through scanning electron microscopy. The results showed that papaya pollen grains were monads, tricolporate, and small and medium-sized. The pollen grains were oblate-spheroidal to spheroidal. Foveolate, reticulate, and fossulate exine ornamentation were observed. Colpi width had maximum coefficient of variation of 25%; Shannon diversity index of all qualitative characters reached over 0.8. Using cluster analysis, 17 papaya cultivars were divided into three groups; there was no relationship between flesh colors and origins. The study findings suggest that pollen morphological analysis is valuable to provide references for breeding and genetic improvement of papaya.
DOI: 10.6084/m9.figshare.20038531
title: Pollen morphological analysis of papaya (Carica papaya L.)

description: Abstract There is little information on pollen morphological analysis of papaya cultivars with different flesh color and origins. We observed pollen morphological characters of 17 papaya cultivars through scanning electron microscopy. The results showed that papaya pollen grains were monads, tricolporate, and small and medium-sized. The pollen grains were oblate-spheroidal to spheroidal. Foveolate, reticulate, and fossulate exine ornamentation were observed. Colpi width had maximum coefficient of variation of 25%; Shannon diversity index of all qualitative characters reached over 0.8. Using cluster analysis, 17 papaya cultivars were divided into three groups; there was no relationship between flesh colors and origins. The study findings suggest that pollen morphological analysis is valuable to provide references for breeding and genetic improvement of papaya.
DOI: 10.6084/m9.figshare.20038533
title: Obtaining okra hybrids through partial diallel analysis

description: Abstract The exploration of heterosis can be an efficient alternative to increase okra yield. The partial diallel strategy optimizes resources by guiding crosses based on genetic diversity between various genotypes. This study aimed to identify potential hybrid combinations through diallel analysis in comparison with commercial cultivars. The hybrids were evaluated by means of a partial diallel scheme based on the genetic divergence of the genotypes. Fourteen hybrids and their reciprocals were evaluated in the winter and summer conditions of Jaboticabal - SP for yield, number of fruits and precocity. No line showed significant general combining ability. The reciprocal effect was not significant for the evaluated characteristics. Four hybrid combinations showed significant and positive specific combining ability for number of fruits, which were considered promising hybrids. In the winter, the experimental hybrids 1 x 8 and 3 x 8 showed superior performance compared to the commercial hybrids. No significant differences were observed in the summer.
DOI: 10.6084/m9.figshare.20038533.v1
title: Obtaining okra hybrids through partial diallel analysis

description: Abstract The exploration of heterosis can be an efficient alternative to increase okra yield. The partial diallel strategy optimizes resources by guiding crosses based on genetic diversity between various genotypes. This study aimed to identify potential hybrid combinations through diallel analysis in comparison with commercial cultivars. The hybrids were evaluated by means of a partial diallel scheme based on the genetic divergence of the genotypes. Fourteen hybrids and their reciprocals were evaluated in the winter and summer conditions of Jaboticabal - SP for yield, number of fruits and precocity. No line showed significant general combining ability. The reciprocal effect was not significant for the evaluated characteristics. Four hybrid combinations showed significant and positive specific combining ability for number of fruits, which were considered promising hybrids. In the winter, the experimental hybrids 1 x 8 and 3 x 8 showed superior performance compared to the commercial hybrids. No significant differences were observed in the summer.
DOI: 10.6084/m9.figshare.20038534
title: BRS Tainá: new white seedless grape cultivar for the Brazilian semi-arid region

description: Abstract The new table grape cultivar BRS Tainá produces white firm seedless grapes, agreeable flavor, balanced sugar-acid ratio, good berry adherence to the pedicel and yield of 50 ton ha-1 year-1. It is recommended for the tropical conditions of the Submédio do Vale do São Francisco. Propagative material is acquired from nurseries licensed by Embrapa.
DOI: 10.6084/m9.figshare.20038534.v1
title: BRS Tainá: new white seedless grape cultivar for the Brazilian semi-arid region

description: Abstract The new table grape cultivar BRS Tainá produces white firm seedless grapes, agreeable flavor, balanced sugar-acid ratio, good berry adherence to the pedicel and yield of 50 ton ha-1 year-1. It is recommended for the tropical conditions of the Submédio do Vale do São Francisco. Propagative material is acquired from nurseries licensed by Embrapa.
DOI: 10.6084/m9.figshare.20038536
title: Mixed model-based Jinks and Pooni method to predict segregating populations in wheat breeding

description: Abstract The improvement of superior wheat cultivars depends on the identification of promising segregating populations to derive superior lines. A lattice model (8×8) involving 56 F2 populations and eight parents was conducted in the 2020 cropping season, and grain yield per plant was evaluated for every F2 population, with further analysis of the population potential by Jinks and Pooni method via REML/BLUP. A total of 5,410 F2 plants were evaluated in this study. The results showed that the use of genetic variance associated with the individual genotypic value (BLUP) was superior compared with the use of variance and traditional phenotypic values. The F2 populations, CD 1303/BRS 254, CD 1303/Tbio Duque, CD 1303/Tbio Ponteiro, BRS 264/Tbio Aton, Tbio Ponteiro/Tbio Aton, and Tbio Sossego/CD 1303 had the highest likelihood of deriving superior lines.
DOI: 10.6084/m9.figshare.20038536.v1
title: Mixed model-based Jinks and Pooni method to predict segregating populations in wheat breeding

description: Abstract The improvement of superior wheat cultivars depends on the identification of promising segregating populations to derive superior lines. A lattice model (8×8) involving 56 F2 populations and eight parents was conducted in the 2020 cropping season, and grain yield per plant was evaluated for every F2 population, with further analysis of the population potential by Jinks and Pooni method via REML/BLUP. A total of 5,410 F2 plants were evaluated in this study. The results showed that the use of genetic variance associated with the individual genotypic value (BLUP) was superior compared with the use of variance and traditional phenotypic values. The F2 populations, CD 1303/BRS 254, CD 1303/Tbio Duque, CD 1303/Tbio Ponteiro, BRS 264/Tbio Aton, Tbio Ponteiro/Tbio Aton, and Tbio Sossego/CD 1303 had the highest likelihood of deriving superior lines.
DOI: 10.6084/m9.figshare.20038540
title: Identification of haploids and diploids in maize using seedling traits and flow cytometry

description: Abstract: The seedling traits is a simple and non-destructive methodology used to identify haploids/diploids in maize. This study aimed at establishing an optimal germination temperature to evaluate this methodology and verify its effectiveness. Haploid and diploid seeds, obtained from crosses with the haploidy inducer TAIL9, were classified using the R1-Navajo marker and placed to germinated in growth chambers maintained at different temperatures. After 96 h, radicle and coleoptile lengths and the number of lateral seminal roots were determined. Cut-off points for these traits were established using Receiver Operating Characteristic curves and a new haploid/diploid classification was performed and compared to the plant phenotype (gold standard) to obtain false discovery rates and false negative rates. The seedling traits methodology successfully differentiated haploids and diploids, proving to be effective in eliminating false positives, selected by the R1-Navajo marker. The temperature of 30 °C was established as the optimal germination temperature for this study.