DOI: 10.5061/dryad.qnk98sfxb
title: Evolution of origin sequence and recognition for licensing of Eukaryotic DNA replication

description: The large size of eukaryotic chromosomes necessitates that the initiation
 of DNA replication occurs at numerous origins of DNA replication. In S.
 cerevisiae, origins are highly DNA sequence-specific and are recognized by
 the Origin Recognition Complex (ORC). In contrast, most eukaryotes have
 lost features in ORC subunits that contribute to DNA sequence-specific
 recognition, raising the question of how origins are identified. An
 analysis of origins in the genome of the distantly related budding yeast
 Yarrowia lipolytica identified considerable variability in origin sequence
 and structure. High-resolution structures demonstrate that Y. lipolytica
 origins are recognized by a combination of ORC and Cdc6 in a manner
 different from S. cerevisiae. The structure of Yarrowia ORC-Cdc6 bound to
 different origins shows considerable plasticity in protein-DNA
 interactions. We compare these yeast structures to the structure of human
 ORC-CDC6 bound to DNA. These studies reveal information on the evolution
 of origins and origin recognition.
description: # Evolution of origin sequence and recognition for licensing of Eukaryotic
 DNA replication Dataset DOI:
 [10.5061/dryad.qnk98sfxb](https://doi.org/10.5061/dryad.qnk98sfxb) ##
 Description of the data and file structure *Yarrowia lipolytica* cells
 were released from stationary phase and were labeled constantly with EDU
 for the indicated minutes with or without hydroxyurea, the DNA was
 isolated and libraries prepared and sequenced.  ### Files and variables
 #### File: 9-30min-eh5_S3_trm_matAfinal.sorted.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 30
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 30min_S4_trm_matAfinal.sorted.bw **Description:** Coverage track of EdU
 labeling with hydroxyurea for 30 minutes aligned to *Y. lipolytica* strain
 E122. #### File: S03_S3_trm_matAfinal.sorted-new45eh.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 45
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 72-45-eh-5_S3_trm_matAfinal.sorted.bw **Description:** Coverage track of
 EdU labeling with hydroxyurea for 45 minutes aligned to *Y. lipolytica*
 strain E122. #### File: S04_S4_trm_matAfinal.sorted-new60eh.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 60
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 S07_S7_trm_matAfinal.sorted-45minold.bw **Description:** Coverage track of
 EdU labeling with hydroxyurea for 45 minutes aligned to *Y. lipolytica*
 strain E122. #### File: S07_S7_trm_matAfinal.sorted-90eh.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 90
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 S09_S9_trm_matAfinal.sorted-75min.bw **Description:** Coverage track of
 EdU labeling with hydroxyurea for 75 minutes aligned to *Y. lipolytica*
 strain E122. #### File:
 S11_S11_trm_matAfinal.sorted-new180-642peakcount.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 180
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 S14_S14_trm_matAfinal.sorted-120min-old.bw **Description:** Coverage track
 of EdU labeling with hydroxyurea for 120 minutes aligned to *Y.
 lipolytica* strain E122. #### File: 45min_S5_trm_matAfinal.sorted.bw
 **Description:** Coverage track of EdU labeling without hydroxyurea for 45
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 S06_S6_trm_matAfinal.sorted.bw **Description:** Coverage track of EdU
 labeling without hydroxyurea for 60 minutes aligned to *Y. lipolytica*
 strain E122. #### File: S08_S8_trm_matAfinal.sorted-new90eh.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 90
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 45min_S6_trm_matAfinal.sorted.bw **Description:** Coverage track of EdU
 labeling without hydroxyurea for 45 minutes aligned to *Y. lipolytica*
 strain E122. #### File: S12_S12_trm_matAfinal.sorted-new210.bw
 **Description:** Coverage track of EdU labeling with hydroxyurea for 210
 minutes aligned to *Y. lipolytica* strain E122. #### File:
 S04_S4_trm_matAfinal.sorted.bw **Description:** Coverage track of EdU
 labeling without hydroxyurea for 60 minutes aligned to *Y. lipolytica*
 strain E122. ## Code/software All coverage tracks can be opened with a
 genome browser using *Y. lipolytica* strain E122 as reference genome.