DOI: 10.5061/dryad.hqbzkh1wq
title: Micromorpho-anatomical studies on the leaves and woods of Turkish <em>Daphne</em> (Thymelaeaceae) taxa

description: The identification of Daphne taxa is difficult because of the lack of
 available morphological characteristics. Here, we aimed to describe leaf
 and wood micromorpho-anatomical characteristics of native Turkish Daphne
 taxa (D. glomerata, D. gnidioides, D. mezereum, D. mucronata, D. oleoides
 subsp. kurdica, D. pontica and D. sericea) from three phytogeographical
 regions (Europeo-Siberian, Irano-Turanian and Mediterranean) from Turkiye
 to contribute to the systematic position of taxa. Totally, 41 leaf and
 wood characters were investigated. Most of the features were characterised
 in detail for the first time in these taxa, and statistical analyses were
 used to evaluate the diagnostic characters. SEM analysis was carried out
 to determine the surface structures of leaves and tracheal elements of
 wood. We encountered the following distinct anatomical differences: (1)
 differences in wax depositions in leaves, (2) in pubescens details, (3) in
 cell wall patterns, (4) in mesophyll types, (5) in midrib structures, (6)
 in stomatal number and stomatal length in hypostomatic leaves with
 anomocytic stomata and (7) in vessel arragement in growth rings, with
 different ray frequency and cellular ray composition, and the presence of
 tori in intervessel pits in wood. An identification key based on these
 micromorpho-anatomical characters is presented, some ecological
 interpretations are made, and the study’s results are compared to existing
 data in the literature. Our results demonstrate that the
 micromorpho-anatomical data across taxa are partly congruent with the
 classification in the Turkish Flora, but new sectional arrangements may be
 recommended for the systematic positions of taxa based on their
 phytogeographical origins.
description: # Micromorpho-anatomical studies on the leaves and woods of Turkish
 *Daphne* (Thymelaeaceae) taxa Dataset DOI:
 [10.5061/dryad.hqbzkh1wq](https://doi.org/10.5061/dryad.hqbzkh1wq) ###
 File: Ozcan___Ersen_Bak_2025__data-statistic-for_MDS.csv
 **Description:** based on the Euclidean distance model and stimulus
 configuration of measures was performed to check the taxonomic
 differentiation of Daphne taxa using leaf and wood anatomical characters
 among the species (Daphne glomerata, D. gnidioides, D. mezereum, D.
 mucronata, D. oleoides subsp. kurdica, D. pontica and D. sericea #####
 Variables .glo: Daphne glomerata .gni: Daphne gnidioides .mez: Daphne
 mezereum .muc: Daphne mucronata .ole: Daphne oleoides subsp. kurdica
 .pont: Daphne pontica .Ser. Daphne sericea .Ct.ad.: Cuticle thickness
 adaxially .ct. Ab.Cuticle thickness abaxially -UE length: Upper epidermal
 length .UE width: Upper epidermal width .md thickness: midrib thickness
 .T. diam.: Trachea diameter .up.pali. Paren.:Upper palisade parenchyma
 thickness -spon.paren.:Spongy parenchyma thickness .le.pali. Paren.:lower
 palisade parenchyma thickness .mesop.thick.:mesophyll thickness .VB
 thickness:Vascular bundle thickness .VB width: Vascular bundle width
 .Xyl.thic.:Xylem thickness .Phl. thic.:Phloem thickness .LE.length: Lower
 epidermal length .LE.width: Lower epidermal width .St. Ind.: Stomatal
 index .St. Length: Stomatal length .St. width: stomatal width .UE coll.
 thic.: Collenchyma thickness in upper side .LE coll. thic.: Collenchyma
 thickness in lower side .epid. numb.: epidermis number per mm2 .st.
 numb.: stomatal number per mm2 .ue shape: upper epidermal shape .le
 shape: lower epidermal shape .mesop. Type:mesophyll type .TD: tangential
 diameter of vessels .RD: radial diameter of vessels .VF: number of vessels
 per mm2 .VWT: vessel wall thickness .VPD: vessel pith diameter
 .VEL: vessel element length .LFL: libriform fibre length .LFW: libriform
 fibre width .LFT: libriform fibre thickness of cell walls .UH: uniseriate
 ray height .UH cell: number of uniceriate cell .U: number of rays per mm2
 .VG: vessel grouping .V: vulnerability ratio .ME: mesomophy value .tr.:
 torous .por. type: Porous type 
 
 ### File: Ozcan___Ersen_Bak_2025__data-statistic-for_UPGMA.csv
 **Description:** 36 were quantitative  and 5 were qualitative
 (ornamentations of adaxial and abaxial epidermal structure, type of
 mesophyll, the presence of tori on the vascular pit membranes, and ring
 porosity). Their groupings were determined using the clustering analysis
 method to  evaluate the  taxonomical relationships of the taxa and
 contribute the systematic. ##### Variables -glo: Daphne glomerata -gni:
 Daphne gnidioides -mez: Daphne mezereum -muc: Daphne mucronata -ole:
 Daphne oleoides subsp. kurdica -pont: Daphne pontica -Ser. Daphne sericea
 -Ct.ad.: Cuticle thickness adaxially -ct. Ab. Cuticle thickness abaxially
 -UE length: Upper epidermal length .UE width: Upper epidermal width .md
 thickness: midrib thickness .T. diam.: Trachea diameter .up.pali.
 Paren.:Upper palisade parenchyma thickness -spon.paren.:Spongy parenchyma
 thickness .le.pali. Paren.:lower palisade parenchyma thickness
 .mesop.thick.:mesophyll thickness .VB thickness:Vascular bundle thickness
 .VB width: Vascular bundle width .Xyl.thic.:Xylem thickness .Phl.
 thic.:Phloem thickness .LE.length: Lower epidermal length .LE.width: Lower
 epidermal width .St. Ind.: Stomatal index .St. Length: Stomatal length
 .St. width: stomatal width .UE coll. thic.: Collenchyma thickness in upper
 side .LE coll. thic.: Collenchyma thickness in lower side .epid.
 numb.: epidermis number per mm2 .st. numb.: stomatal number per mm2 .ue
 shape: upper epidermal shape .le shape: lower epidermal shape .mesop.
 Type:mesophyll type .TD: tangential diameter of vessels .RD: radial
 diameter of vessels .VF: number of vessels per mm2 .VWT: vessel wall
 thickness .VPD: vessel pith diameter .VEL: vessel element length
 .LFL: libriform fibre length .LFW: libriform fibre width .LFT: libriform
 fibre thickness of cell walls .UH: uniseriate ray height .UH cell: number
 of uniceriate cell .U: number of rays per mm2 .VG: vessel grouping
 .V: vulnerability ratio .ME: mesomophy value .tr.: torous .por.
 type: Porous type 
 
 ### File: Ozcan___Ersen_Bak_2025__data-statistic-homojenite_tests.xls
 **Description:** Used to test whether the data obtained leaf and wood
 anatomies of the taxa is normally distribution. ##### Variables
 -Ct.ad.: Cuticle thickness adaxially -ct. Ab. Cuticle thickness abaxially
 -UE length: Upper epidermal length .UE width: Upper epidermal width .md
 thickness: midrib thickness .T. diam.: Trachea diameter .up.pali.
 Paren.:Upper palisade parenchyma thickness -spon.paren.:Spongy parenchyma
 thickness .le.pali. Paren.:lower palisade parenchyma thickness
 .mesop.thick.:mesophyll thickness .VB thickness:Vascular bundle thickness
 .VB width: Vascular bundle width .Xyl.thic.:Xylem thickness .Phl.
 thic.:Phloem thickness .LE.length: Lower epidermal length .LE.width: Lower
 epidermal width .St. Ind.: Stomatal index .St. Length: Stomatal length
 .St. width: stomatal width .UE coll. thic.: Collenchyma thickness in upper
 side .LE coll. thic.: Collenchyma thickness in lower side .epid.
 numb.: epidermis number per mm2 .st. numb.: stomatal number per mm2