cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19923146.v1
title: Estimating the Electrochemically Active Area: Revisiting a Basic Concept in Electrochemistry

description: The well-known electrochemical probe Fe(CN)63-/Fe(CN)64- is widely used for estimating the electrochemically active area of electrodes modified with carbon nanotubes, conductive polymers, enzymes, etc. In this study, we used the platinum electrode, smooth or platinized with different roughness factors, to demonstrate that such a redox couple fails to respond to a surface roughness variation. We determined the roughness factors of the Pt surfaces by atomic force microscopy (AFM) images, which yielded values between 2.72 and 25.91. Almost the same values were found by using the charge of the hydrogen monolayer desorption obtained from steady-state cyclic voltammetry experiments performed in an acid medium. They were then compared with those provided by peak current in voltammetry or chronoamperometry with Fe(CN)63-/Fe(CN)64- which all yielded values nearly to one. Such comparison demonstrates that the electrochemical behavior of the redox probe is an outer sphere reaction with a quite small interaction with the electrode surface, thus not being suitable to be related with active areas.
DOI: 10.6084/m9.figshare.19923148
title: Octadecanal as the Male-Produced Aggregation Pheromone of the Coconut Weevil, Amerrhinus ynca (Coleoptera: Curculionidae)

description: The beetle Amerrhinus ynca Sahlberg, 1823 (Coleoptera: Curculionidae) causes damage to several species of the Arecaceae family. The larvae of this palm weevil damage palm trees by causing perforations in the leaf rachis, obstructing the flow of nutrients to the plant. To date, are no registered insecticides for the control of A. ynca. An efficient control strategy would be the use of synthetic pheromones of this beetle for integrated pest management tactics. In this work, the aggregation pheromone of A. ynca was identified from adults collected as larvae and pupae from the rachis of coconut palm. The volatile compounds produced by A. ynca were collected via headspace aeration of adult beetles, and the identification of the pheromone was performed with gas chromatography coupled to mass spectrometry (GC-MS), GC-Fourier transform infrared spectroscopy (GC-FTIR) and GC-electroantenographic detector (GC-EAD). Dual-choice olfactometry experiments were performed to evaluate the bioactivity of the synthetic pheromone. GC analyses showed the presence of a male-specific compound that was identified as octadecanal. In GC-EAD analyses, synthetic octadecanal elicited responses of A. ynca female antennae. Behavioral bioassays showed attractiveness of synthetic compound for males and females, indicating octadecanal as the aggregation pheromone of A. ynca.
DOI: 10.6084/m9.figshare.19923148.v1
title: Octadecanal as the Male-Produced Aggregation Pheromone of the Coconut Weevil, Amerrhinus ynca (Coleoptera: Curculionidae)

description: The beetle Amerrhinus ynca Sahlberg, 1823 (Coleoptera: Curculionidae) causes damage to several species of the Arecaceae family. The larvae of this palm weevil damage palm trees by causing perforations in the leaf rachis, obstructing the flow of nutrients to the plant. To date, are no registered insecticides for the control of A. ynca. An efficient control strategy would be the use of synthetic pheromones of this beetle for integrated pest management tactics. In this work, the aggregation pheromone of A. ynca was identified from adults collected as larvae and pupae from the rachis of coconut palm. The volatile compounds produced by A. ynca were collected via headspace aeration of adult beetles, and the identification of the pheromone was performed with gas chromatography coupled to mass spectrometry (GC-MS), GC-Fourier transform infrared spectroscopy (GC-FTIR) and GC-electroantenographic detector (GC-EAD). Dual-choice olfactometry experiments were performed to evaluate the bioactivity of the synthetic pheromone. GC analyses showed the presence of a male-specific compound that was identified as octadecanal. In GC-EAD analyses, synthetic octadecanal elicited responses of A. ynca female antennae. Behavioral bioassays showed attractiveness of synthetic compound for males and females, indicating octadecanal as the aggregation pheromone of A. ynca.
DOI: 10.6084/m9.figshare.19923152
title: Perceptions and use of medicinal plants by an elementary and high school community in Vila Velha, Espírito Santo, Brazil

description: Plants have been used for centuries as therapeutic resources for various pathological conditions and are occasionally considered as the only resource available to some communities. However, knowledge on the potential of traditional medicinal plants have been lost over the years. Strategies that can guarantee the prospection of popular knowledge regarding medicinal plants to stimulate the spread of information between generations are therefore of enormous value. Thus, the present study aimed to investigate the use and knowledge on medicinal plants of students from a public school in Vila Velha (Brazil) and their associated community, including parents. In this urban locality, data were obtained by the application of a semi-structured questionnaire to 206 people from the school community. Although homegrown cultivation seems to be an uncommon practice in urban contexts, the use of medicinal plants by the sampled population was significant (80%), as well as the interviewees’ confidence on its therapeutic action (90%). It is worth mentioning that more than 70% of the respondents presented their parents and/or grandparents as their main source of information, highlighting the importance of popular knowledge valorization.
DOI: 10.6084/m9.figshare.19923152.v1
title: Perceptions and use of medicinal plants by an elementary and high school community in Vila Velha, Espírito Santo, Brazil

description: Plants have been used for centuries as therapeutic resources for various pathological conditions and are occasionally considered as the only resource available to some communities. However, knowledge on the potential of traditional medicinal plants have been lost over the years. Strategies that can guarantee the prospection of popular knowledge regarding medicinal plants to stimulate the spread of information between generations are therefore of enormous value. Thus, the present study aimed to investigate the use and knowledge on medicinal plants of students from a public school in Vila Velha (Brazil) and their associated community, including parents. In this urban locality, data were obtained by the application of a semi-structured questionnaire to 206 people from the school community. Although homegrown cultivation seems to be an uncommon practice in urban contexts, the use of medicinal plants by the sampled population was significant (80%), as well as the interviewees’ confidence on its therapeutic action (90%). It is worth mentioning that more than 70% of the respondents presented their parents and/or grandparents as their main source of information, highlighting the importance of popular knowledge valorization.
DOI: 10.17632/nrchz3wc88.1
title: Chironomid dataset from Mutterbergersee: A late-Holocene paleotemperature proxy record for the Central Eastern Alps, Austria

description: Data have been derived from the investigation of long-term (AD 1300–2010) changes in chironomid (non-biting midges) assemblages in sediment core MUT-10, retrieved from Mutterbergersee (MUT), a high-alpine lake in the Central Eastern Alps (Stubai Alps, Tyrol). The data are provided in five separate Excel sheets. The first sheet (metadata) provides information about the coring site, archive type and related research articles. The second one (raw chironomid count) contains subfossil chironomid head capsule count data (= number of head capsules recovered per sample). The third one (chironomid percentages) contains the relative abundances of each taxon calculated with respect to the total chironomid remains enumerated per sample. The fourth sheet (chironomid-inferred T_July) includes mean July air temperature data calculated from the chironomid assemblages using a Swiss-Norwegian chironomid–temperature inference model. The chronological framework data are available in the fifth sheet (dating) containing information on the 210Pb and 14C measurements in sediment core MUT-10.
DOI: 10.17632/6kz2rv4g6j.1
title: LCPT vs MR-4

description: Comparison of two once-daily administered tacrolimus formulations.
DOI: 10.17632/cz7xmyr2cv.1
title: Mouse mRNA sequencing counts

description: mouse mRNA
DOI: 10.17632/jsp4pphmxm.1
title: Raw data for establishing a mathematical model of atherosclerosis staging based on lipidomics

description: This data is the test results of serum lipid metabolites in ApoE-/- mice and C57BL/6 mice fed with normal diet at different ages (4W, 8W, 12W, 16W, 20W, 24W, 28W, 32W). 4WC57 represents the 4-week-old The peak area of C57BL/6mice lipid metabolites, 4WAP represents the peak area of lipid metabolites in 4-week-old ApoE-/- mice, with 10 replicates in each group, and other sample names are analogous. After pre-processing the raw mass spectrometric data by removing the background noise, peak extraction, deconvolution, peak alignment, alignment, merging, and noise removal after peak merging, gap filling, data export using the data mining software MZmine 2.10, we identified 732 lipid components. Area represents the peak area. The NAME line represents the name of the lipid metabolite. QC samples are quality control samples that are mixed with several samples as a constant standard, and 2 QCs are taken every 20 samples.
DOI: 10.17632/x2gwmgnzxv.1
title: Facile method for synthesizing a magnetically removable visible-light photocatalyst based on nickel doped zinc ferrite 

description: Raw and processed data employed in the work "Facile method for synthesizing a magnetically removable visible-light photocatalyst based on nickel doped zinc ferrite "
DOI: 10.17632/pgs49k868z.1
title: A diminished immune response underlies age-related SARS-CoV-2 pathologies. Oishi and Horiuchi et al

description: Supplementary Tables
DOI: 10.17632/mffncst8fw.1
title: Natural VTA activity during NREM sleep influences future exploratory behavior

description: Data for the project Natural VTA activity during NREM sleep influences future exploratory behaviour. Individual data descriptions given in each folder.
DOI: 10.6084/m9.figshare.19922096
title: Supplementary Material for: Validation of a Protocol for Continuous Hemodiafiltration with Regional Citrate Anticoagulation with Omni®

description: <b><i>Introduction:</i></b> Omni® (B Braun, Melsungen, Germany) is able to run continuous renal replacement therapy (CRRT) in continuous veno-venous hemofiltration (CVVH), hemodialysis (CVVHD), and hemodiafiltration (CVVHDF) modes. However, to date, there is no validated protocol to guide the use of Omni® in CVVHDF mode with regional citrate anticoagulation (RCA). <b><i>Methods:</i></b> We designed a protocol for CVVHDF-RCA tailored for Omni®. This protocol was tested in patients included in an observational study conducted in our center between January and March 2021. For all study patients, we collected baseline characteristics, laboratory results, CRRT circuit lifespan as well as plasma and effluent samples at 12, 24, 48, and 72 h of CRRT circuit initiation. At each study time point, we computed urea, creatinine, and β2-microglobulin clearance as well as effluent/blood ratios. Data from circuits in CVVHDF-RCA mode are compared with those in standard therapy (CVVHD-RCA) with the same device. <b><i>Results:</i></b> We analyzed ten circuits (5 patients) in CVVHDF-RCA mode and 32 (13 patients) in CVVHD-RCA mode. No adverse events related to the therapy were observed. In CVVHDF-RCA mode, median circuit running time was 68 (IQR 8.1) hours versus 46 (IQR 9.0) in CVVHD mode, <i>p</i> = 0.053. Therapy adaptations (dialysate rate and/or blood flow) were required in one (10%) circuit (15.6% in CVVHD mode, <i>p</i> = 0.56). Compared to CVVHD, CVVHDF was able to achieve similar clearance and effluent/blood ratio for urea, creatinine, and β2-microglobulin across the entire duration of circuit lifetime. <b><i>Conclusion:</i></b> The proposed protocol for CVVHDF-RCA for Omni® was associated with similar circuit lifetime, number of required adaptations and clearances to standard CVVHD-RCA. It appears to be safe and feasible.
DOI: 10.6084/m9.figshare.19923158.v1
title: Continuing health education for qualification of professional practice in Psychosocial Care Centers

description: ABSTRACT Objective: to describe and analyze a strategy of continuing health education to manage the quality of professionals’ work in Psychosocial Care Centers. Method: this is a research-intervention carried out in Psychosocial Care Centers in the state of Goiás, Brazil, with the participation of 58 professionals. Data were collected in 2016 through seminars and workshops. Thematic content analysis was carried out. Results: professionals associated the Singular Therapeutic Project to the record of performed procedures and described the need to develop skills for correct completion and interpretation of procedures, use of a record software and computerization of processes. The qualification strategy used was considered to be effective in making improvements to the work carried out feasible. Final Considerations: the study presents a qualification strategy for community mental health service teams to guide the care model for territorial care centered on users.
DOI: 10.6084/m9.figshare.19923158
title: Continuing health education for qualification of professional practice in Psychosocial Care Centers

description: ABSTRACT Objective: to describe and analyze a strategy of continuing health education to manage the quality of professionals’ work in Psychosocial Care Centers. Method: this is a research-intervention carried out in Psychosocial Care Centers in the state of Goiás, Brazil, with the participation of 58 professionals. Data were collected in 2016 through seminars and workshops. Thematic content analysis was carried out. Results: professionals associated the Singular Therapeutic Project to the record of performed procedures and described the need to develop skills for correct completion and interpretation of procedures, use of a record software and computerization of processes. The qualification strategy used was considered to be effective in making improvements to the work carried out feasible. Final Considerations: the study presents a qualification strategy for community mental health service teams to guide the care model for territorial care centered on users.
DOI: 10.6084/m9.figshare.19923163
title: Chromogenic Chemosensors Based on Phenolic Imines for the Detection of Alkylamines and Lidocaine in Water and in the Vapor Phase

description: Three imines comprised of 5-nitrothiophen-2-yl as electron-accepting and phenols as electron-donating groups were synthesized and used as chromogenic chemosensors to detect alkylamines. The compounds are colorless in water, but their deprotonation by the alkylamines generates the corresponding colored phenolates, which can be used to detect those analytes. The addition of cetyltrimethylammonium bromide (CTAB) causes bathochromic shift of the perichromic band of the phenolates, indicating that the compounds are transferred into the micelles. In addition, CTAB lowers the pKa of phenols and increases the stability of the phenolates, improving their performance as chemosensors. Applications were prospected with the chromogenic chemosensors adsorbed on strip papers to detect alkylamines in water and in the vapor phase. The compounds were also used in solution for the quantification of lidocaine in water. Thus, the versatility of the compounds studied allows to think about applications in industrial, environmental, and pharmaceutical areas.
DOI: 10.6084/m9.figshare.19923163.v1
title: Chromogenic Chemosensors Based on Phenolic Imines for the Detection of Alkylamines and Lidocaine in Water and in the Vapor Phase

description: Three imines comprised of 5-nitrothiophen-2-yl as electron-accepting and phenols as electron-donating groups were synthesized and used as chromogenic chemosensors to detect alkylamines. The compounds are colorless in water, but their deprotonation by the alkylamines generates the corresponding colored phenolates, which can be used to detect those analytes. The addition of cetyltrimethylammonium bromide (CTAB) causes bathochromic shift of the perichromic band of the phenolates, indicating that the compounds are transferred into the micelles. In addition, CTAB lowers the pKa of phenols and increases the stability of the phenolates, improving their performance as chemosensors. Applications were prospected with the chromogenic chemosensors adsorbed on strip papers to detect alkylamines in water and in the vapor phase. The compounds were also used in solution for the quantification of lidocaine in water. Thus, the versatility of the compounds studied allows to think about applications in industrial, environmental, and pharmaceutical areas.
DOI: 10.6084/m9.figshare.19923164
title: Optimal culture conditions for neurosphere formation and neuronal differentiation from human dental pulp stem cells

description: Abstract Objectives Human dental pulp stem cells (DPSCs) have been used to regenerate damaged nervous tissues. However, the methods of committing DPSCs into neural stem/progenitor cells (NSPCs) or neurospheres are highly diverse, resulting in many neuronal differentiation outcomes. This study aims to validate an optimal protocol for inducing DPSCs into neurospheres and neurons. Methodology After isolation and characterization of mesenchymal stem cell identity, DPSCs were cultured in a NSPC induction medium and culture vessels. The durations of the culture, dissociation methods, and passage numbers of DPSCs were varied. Results Neurosphere formation requires a special surface that inhibits cell attachment. Five-days was the most appropriate duration for generating proliferative neurospheres and they strongly expressed Nestin, an NSPC marker. Neurosphere reformation after being dissociated by the Accutase enzyme was significantly higher than other methods. Passage number of DPSCs did not affect neurosphere formation, but did influence neuronal differentiation. We found that the cells expressing a neuronal marker, β-tubulin III, and exhibiting neuronal morphology were significantly higher in the early passage of the DPSCs. Conclusion These results suggest a guideline to obtain a high efficiency of neurospheres and neuronal differentiation from DPSCs for further study and neurodegeneration therapeutics.
DOI: 10.6084/m9.figshare.19923164.v1
title: Optimal culture conditions for neurosphere formation and neuronal differentiation from human dental pulp stem cells

description: Abstract Objectives Human dental pulp stem cells (DPSCs) have been used to regenerate damaged nervous tissues. However, the methods of committing DPSCs into neural stem/progenitor cells (NSPCs) or neurospheres are highly diverse, resulting in many neuronal differentiation outcomes. This study aims to validate an optimal protocol for inducing DPSCs into neurospheres and neurons. Methodology After isolation and characterization of mesenchymal stem cell identity, DPSCs were cultured in a NSPC induction medium and culture vessels. The durations of the culture, dissociation methods, and passage numbers of DPSCs were varied. Results Neurosphere formation requires a special surface that inhibits cell attachment. Five-days was the most appropriate duration for generating proliferative neurospheres and they strongly expressed Nestin, an NSPC marker. Neurosphere reformation after being dissociated by the Accutase enzyme was significantly higher than other methods. Passage number of DPSCs did not affect neurosphere formation, but did influence neuronal differentiation. We found that the cells expressing a neuronal marker, β-tubulin III, and exhibiting neuronal morphology were significantly higher in the early passage of the DPSCs. Conclusion These results suggest a guideline to obtain a high efficiency of neurospheres and neuronal differentiation from DPSCs for further study and neurodegeneration therapeutics.
DOI: 10.6084/m9.figshare.19922348
title: Supplementary Material for: Skewed X-Chromosome Inactivation as a Possible Marker of X-Linked CNV in Women with Pregnancy Loss

description: Skewed X-chromosome inactivation (sXCI) can be a marker of lethal genetic variants on the X chromosome in a woman since sXCI modifies the pathological phenotype. The aim of this study was to search for CNVs in women with miscarriages and sXCI. XCI was assayed using the classical method based on the amplification of highly polymorphic exon 1 of the androgen receptor (<i>AR</i>) gene. The XCI status was analysed in 313 women with pregnancy loss and in 87 spontaneously aborted embryos with 46,XX karyotype, as well as in control groups of 135 women without pregnancy loss and 64 embryos with 46,XX karyotype from induced abortions in women who terminated a normal pregnancy. The frequency of sXCI differed significantly between women with miscarriages and women without pregnancy losses (6.3% and 2.2%, respectively; <i>p</i> = 0.019). To exclude primary causes of sXCI, sequencing of the <i>XIST</i> and <i>XACT</i> genes was performed. The <i>XIST</i> and <i>XACT</i> gene sequencing revealed no known pathogenic variants that could lead to sXCI. Molecular karyotyping was performed using aCGH, followed by verification of X-linked CNVs by RT-PCR and MLPA. Microdeletions at Xp11.23 and Xq24 as well as gains of Xq28 were detected in women with sXCI and pregnancy loss.
DOI: 10.6084/m9.figshare.19922348.v1
title: Supplementary Material for: Skewed X-Chromosome Inactivation as a Possible Marker of X-Linked CNV in Women with Pregnancy Loss

description: Skewed X-chromosome inactivation (sXCI) can be a marker of lethal genetic variants on the X chromosome in a woman since sXCI modifies the pathological phenotype. The aim of this study was to search for CNVs in women with miscarriages and sXCI. XCI was assayed using the classical method based on the amplification of highly polymorphic exon 1 of the androgen receptor (<i>AR</i>) gene. The XCI status was analysed in 313 women with pregnancy loss and in 87 spontaneously aborted embryos with 46,XX karyotype, as well as in control groups of 135 women without pregnancy loss and 64 embryos with 46,XX karyotype from induced abortions in women who terminated a normal pregnancy. The frequency of sXCI differed significantly between women with miscarriages and women without pregnancy losses (6.3% and 2.2%, respectively; <i>p</i> = 0.019). To exclude primary causes of sXCI, sequencing of the <i>XIST</i> and <i>XACT</i> genes was performed. The <i>XIST</i> and <i>XACT</i> gene sequencing revealed no known pathogenic variants that could lead to sXCI. Molecular karyotyping was performed using aCGH, followed by verification of X-linked CNVs by RT-PCR and MLPA. Microdeletions at Xp11.23 and Xq24 as well as gains of Xq28 were detected in women with sXCI and pregnancy loss.