cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.17092345
title: Molecular epidemiology and pathogenicity of H5N1 and H9N2 avian influenza viruses in clinically affected chickens on farms in Bangladesh

description: Avian influenza virus (AIV) subtypes H5N1 and H9N2 co-circulate in poultry in Bangladesh, causing significant bird morbidity and mortality. Despite their importance to the poultry value chain, the role of farms in spreading and maintaining AIV infections remains poorly understood in most disease-endemic settings. To address this crucial gap, we conducted a cross-sectional study between 2017 and 2019 in the Chattogram Division of Bangladesh in clinically affected and dead chickens in farms with suspected AIV infection. Viral prevalence of each subtype was approximately 10% among farms for which veterinary advice was sought, indicating high levels of virus circulation in chicken farms despite the low number of reported outbreaks. Co-circulation of both subtypes was common in farms, with our findings suggest that in the field, the co-circulation of H5N1 and H9N2 can modulate disease severity, which could facilitate an underestimated level of AIV transmission in the poultry value chain. Finally, using newly generated whole-genome sequences, we investigate the evolutionary history of a small subset of H5N1 and H9N2 viruses. Our analyses revealed that for both subtypes, the sampled viruses were genetically most closely related to other viruses isolated in Bangladesh and represented multiple independent incursions. However, due to lack of longitudinal surveillance in this region, it is difficult to ascertain whether these viruses emerged from endemic strains circulating in Bangladesh or from neighbouring countries. We also show that amino acids at putative antigenic residues underwent a distinct replacement during 2012 which coincides with the use of H5N1 vaccines.
DOI: 10.6084/m9.figshare.17092345.v1
title: Molecular epidemiology and pathogenicity of H5N1 and H9N2 avian influenza viruses in clinically affected chickens on farms in Bangladesh

description: Avian influenza virus (AIV) subtypes H5N1 and H9N2 co-circulate in poultry in Bangladesh, causing significant bird morbidity and mortality. Despite their importance to the poultry value chain, the role of farms in spreading and maintaining AIV infections remains poorly understood in most disease-endemic settings. To address this crucial gap, we conducted a cross-sectional study between 2017 and 2019 in the Chattogram Division of Bangladesh in clinically affected and dead chickens in farms with suspected AIV infection. Viral prevalence of each subtype was approximately 10% among farms for which veterinary advice was sought, indicating high levels of virus circulation in chicken farms despite the low number of reported outbreaks. Co-circulation of both subtypes was common in farms, with our findings suggest that in the field, the co-circulation of H5N1 and H9N2 can modulate disease severity, which could facilitate an underestimated level of AIV transmission in the poultry value chain. Finally, using newly generated whole-genome sequences, we investigate the evolutionary history of a small subset of H5N1 and H9N2 viruses. Our analyses revealed that for both subtypes, the sampled viruses were genetically most closely related to other viruses isolated in Bangladesh and represented multiple independent incursions. However, due to lack of longitudinal surveillance in this region, it is difficult to ascertain whether these viruses emerged from endemic strains circulating in Bangladesh or from neighbouring countries. We also show that amino acids at putative antigenic residues underwent a distinct replacement during 2012 which coincides with the use of H5N1 vaccines.
DOI: 10.15468/dl.9cz4sr
title: Occurrence Download

description: A dataset containing 319 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tylophora indica (Burm.fil.) Merr."
 ]
}

 The dataset includes 319 records from 34 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030869-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29066489
title: CENTRIL-D-Elevated Senescence Markers in Developing Trisomy 21 Human Lungs

description: Human chromosomal anomalies, notably trisomies, disrupt gene expression, leading to diverse cellular and organ phenotypes. Increased cellular senescence (SEN) and oxidative stress in trisomies have gained recent attention. We assessed SEN, senescence-associated secretory phenotype (SASP) and oxidative stress on trisomy 13, 18, and 21 (T13, T18, T21) human fetal lung tissues and isolated primary human fetal lung fibroblasts. Telomerase associated foci (TAF) staining showed DNA damage primarily within T21 and T18 lungs. These results were confirmed by RT-qPCR showing an increase of the SEN marker <i>CDKN2B</i> and SASP markers <i>IL-6</i> and <i>CXCL8</i>. In contrast, lung tissues from T13 showed an upregulation of <i>CDKN2A</i>, while no significant changes in SASP marker genes were observed. γ-H2AX was upregulated in each genotype, particularly in T21. Isolated fibroblasts demonstrated a strong association between T21 and several SEN markers. An increase of γ-H2AX positive cells were observed in fibroblasts from T21, T18 and T13, but only T21 exhibited an elevation in P21 expression. Solely T21 fibroblasts displayed a significant increase in reactive oxygen species (ROS) levels, as indicated by MitoSOX and CellROX. This study provides the first evidence of a link between SEN and trisomy anomalies during prenatal human lung development, particularly in T21.
DOI: 10.26300/0b21-ef09
title: If I Speak Music — A compositional adventure
DOI: 10.15468/dl.nf5z7b
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lycopodiophyta"
 ]
}

 The dataset includes 21 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030871-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15190230
title: ProtBAG_Figures_Scripts_Dataset

description: This repository will share the original figures, part of the scripts, and the large-scale dataset that I could not include in the Supplementary Materials.
DOI: 10.5281/zenodo.15190229
title: ProtBAG_Figures_Scripts_Dataset

description: This repository will share the original figures, the scripts for my methodological experiments, and the large-scale dataset I could not include in the Supplementary Materials.
DOI: 10.5281/zenodo.15191207
title: ProtBAG_Figures_Scripts_Dataset

description: This repository will share the original figures, part of the scripts, and the large-scale dataset that I could not include in the Supplementary Materials.
DOI: 10.5281/zenodo.15211957
title: ProtBAG_Figures_Scripts_Dataset

description: This repository will share the original figures, the scripts for my methodological experiments, and the large-scale dataset I could not include in the Supplementary Materials.
DOI: 10.5281/zenodo.15413026
title: ProtBAG_Figures_Scripts_Dataset

description: This repository will share the original figures, the scripts for my methodological experiments, and the large-scale dataset I could not include in the Supplementary Materials.
DOI: 10.7939/r3dz03319
title: Takeover Defenses 2013

description: This item consists of two files: the dataset and the dataset list (a key to the fields in the dataset file).
DOI: 10.15468/dl.kuf642
title: Occurrence Download

description: A dataset containing 103 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Philippines",
 "Geometry POLYGON((118.61173 9.78214,118.66489 9.78288,118.65932 9.8115,118.61508 9.81225,118.61173 9.78214))",
 "OccurrenceStatus is Present",
 "TaxonKey is Plantae"
 ]
}

 The dataset includes 103 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030873-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.60859/1afz-sc66
title: NG00127v1.0
DOI: 10.5281/zenodo.15215413
title: RPE1-E150-Benchmarking

description: Processed dataset of all essential gene CRISPRi perturbations within RPE-1 cells (benchmarking overloading) from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including.

1) guide umis per cell: rpe1_e150_maxol_exp_norm_crispri_aggr_total_guide_umis.h5

2) final qc filtered population with multiplets: rpe1_e150_maxol_crispri_scanpy_from_cellranger_aggr_singlets_and_multiplets_5umi_cellranger_guide_calls.h5ad

3) regression model benchmarking inputs: RPE1_E150_all_genes.h5ad, rpe1_e150_maxol_crispri_full_design_matrix_guide_identity.csv
DOI: 10.5281/zenodo.15215414
title: RPE1-E150-Benchmarking

description: Processed dataset of all essential gene CRISPRi perturbations within RPE-1 cells (benchmarking overloading) from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including.

1) guide umis per cell: rpe1_e150_maxol_exp_norm_crispri_aggr_total_guide_umis.h5

2) final qc filtered population with multiplets: rpe1_e150_maxol_crispri_scanpy_from_cellranger_aggr_singlets_and_multiplets_5umi_cellranger_guide_calls.h5ad

3) regression model benchmarking inputs: RPE1_E150_all_genes.h5ad, rpe1_e150_maxol_crispri_full_design_matrix_guide_identity.csv
DOI: 10.5281/zenodo.15213596
title: Hs27-CRISPRa-TFs cellranger outputs

description: Processed cellranger outputs of all transcription factor CRISPRa perturbations within Hs27 cells from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains cellranger outputs for each 10x lane within the experiment.
DOI: 10.5281/zenodo.15213597
title: Hs27-CRISPRa-TFs cellranger outputs

description: Processed cellranger outputs of all transcription factor CRISPRa perturbations within Hs27 cells from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains cellranger outputs for each 10x lane within the experiment.
DOI: 10.5281/zenodo.15215388
title: RPE1-E150-Benchmarking cellranger outputs

description: Cellranger outputs of all essential gene CRISPRi perturbations within RPE-1 cells (benchmarking overloading) from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254
DOI: 10.5281/zenodo.15215389
title: RPE1-E150-Benchmarking cellranger outputs

description: Cellranger outputs of all essential gene CRISPRi perturbations within RPE-1 cells (benchmarking overloading) from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254
DOI: 10.5281/zenodo.15215153
title: K562 dCas9-CUT&RUN

description: Processed dCas9 directed CUT&RUN in K562 cells with varying protospacer targets from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254
DOI: 10.5281/zenodo.15215154
title: K562 dCas9-CUT&RUN

description: Processed dCas9 directed CUT&RUN in K562 cells with varying protospacer targets from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254
DOI: 10.5281/zenodo.15215215
title: RPE-1 and Hs27 CUT&RUN, ATAC-seq, and RNA-seq characterization

description: Processed bulk datasets characterizing Hs27 (fibroblast) RPE-1 cell lines from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains ATAC-seq, CUT&RUN, and Bulk RNA-seq of Hs27 and RPE-1 cell lines
DOI: 10.5281/zenodo.15215216
title: RPE-1 and Hs27 CUT&RUN, ATAC-seq, and RNA-seq characterization

description: Processed bulk datasets characterizing Hs27 (fibroblast) RPE-1 cell lines from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains ATAC-seq, CUT&RUN, and Bulk RNA-seq of Hs27 and RPE-1 cell lines
DOI: 10.5281/zenodo.15213618
title: RPE1-CRISPRa-TFs

description: Processed dataset of all transcription factor CRISPRa perturbations within RPE-1 cells from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including.

1) guide umis per cell: RPE1_CRISPRa_aggr_total_guide_umis.h5

2) final qc filtered population with multiplets: RPE1_CRISPRa_final_population.h5ad

3) filtered singlets, processed with scanpy.pp.normalized_total and scanpy.pp.log1p. Raw counts saved in layer['counts']: RPE1_CRISPRa_final_population_singlets_normalized_log1p.h5ad

4) "mean" population constructed from the full regression model from overloaded droplets: RPE1_CRISPRa_mean_pop.h5ad
DOI: 10.5281/zenodo.15213619
title: RPE1-CRISPRa-TFs

description: Processed dataset of all transcription factor CRISPRa perturbations within RPE-1 cells from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including.

1) guide umis per cell: RPE1_CRISPRa_aggr_total_guide_umis.h5

2) final qc filtered population with multiplets: RPE1_CRISPRa_final_population.h5ad

3) filtered singlets, processed with scanpy.pp.normalized_total and scanpy.pp.log1p. Raw counts saved in layer['counts']: RPE1_CRISPRa_final_population_singlets_normalized_log1p.h5ad

4) "mean" population constructed from the full regression model from overloaded droplets: RPE1_CRISPRa_mean_pop.h5ad
DOI: 10.5281/zenodo.15211971
title: RPE1-CRISPRa-TFs cellranger outputs

description: Processed cellranger outputs of all transcription factor CRISPRa perturbations within RPE-1 cells from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA

This record contains cellranger outputs for each 10x lane within the experiment.
DOI: 10.5281/zenodo.15211972
title: RPE1-CRISPRa-TFs cellranger outputs

description: Processed cellranger outputs of all transcription factor CRISPRa perturbations within RPE-1 cells from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA

This record contains cellranger outputs for each 10x lane within the experiment.
DOI: 10.5281/zenodo.15200178
title: Hs27-CRISPRa-TFs

description: Processed dataset of all transcription factor CRISPRa perturbations within Hs27 cells (fibroblasts) from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including.

1) guide umis per cell: fibroblast_CRISPRa_aggr_total_guide_umis.h5

2) final qc filtered population with multiplets: fibroblast_CRISPRa_final_pop.h5ad

3) filtered singlets, processed with scanpy.pp.normalized_total and scanpy.pp.log1p. Raw counts saved in layer['counts']: fibroblast_CRISPRa_final_pop_singlets_normalized_log1p.h5ad

4) "mean" population constructed from the full regression model from overloaded droplets: fibroblast_CRISPRa_mean_pop.h5ad
DOI: 10.5281/zenodo.15200179
title: Hs27-CRISPRa-TFs

description: Processed dataset of all transcription factor CRISPRa perturbations within Hs27 cells (fibroblasts) from:

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states

Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553.

in press at Nature Genetics

Raw fastq files available at SRA: PRJNA1108254

This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including.

1) guide umis per cell: fibroblast_CRISPRa_aggr_total_guide_umis.h5

2) final qc filtered population with multiplets: fibroblast_CRISPRa_final_pop.h5ad

3) filtered singlets, processed with scanpy.pp.normalized_total and scanpy.pp.log1p. Raw counts saved in layer['counts']: fibroblast_CRISPRa_final_pop_singlets_normalized_log1p.h5ad

4) "mean" population constructed from the full regression model from overloaded droplets: fibroblast_CRISPRa_mean_pop.h5ad
DOI: 10.6084/m9.figshare.14394174
title: Emergence of new phylogenetic lineage of Influenza D virus with broad antigenicity in California, United States

description: Influenza D virus (IDV), with bovines as a primary host, circulates widely in cattle populations across North America and Eurasia. Here we report the identification of a novel IDV group with broad antigenicity in U.S. bovine herds, which is genetically different from previously known lineages of IDV.
DOI: 10.6084/m9.figshare.14394174.v1
title: Emergence of new phylogenetic lineage of Influenza D virus with broad antigenicity in California, United States

description: Influenza D virus (IDV), with bovines as a primary host, circulates widely in cattle populations across North America and Eurasia. Here we report the identification of a novel IDV group with broad antigenicity in U.S. bovine herds, which is genetically different from previously known lineages of IDV.
DOI: 10.15468/dl.bz65sy
title: Occurrence Download

description: A dataset containing 1601 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Clinopodium coccineum (Nutt. ex Hook.) Kuntze"
 ]
}

 The dataset includes 1601 records from 92 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030874-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29066588
title: DATOS CUANTITATIVOS.xlsx

description: Organización de los datos cuantitativos recogidos durante el estudio: Impacto de Modelos de Lenguaje Grande Multimodal (LMM) con Visión de Pantalla frente a LMM Sin Visión de pantalla en la Educación Superior: Un Estudio Cuasi-experimental.