cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/rd2gc-nje41
title: Vibrio cholerae delta TcpB

description: Raw data files of Vibrio cholerae delta TcpB
description: <strong>Tilt Series Date:</strong> 2015-10-16</p>
<strong>Data Taken By:</strong> Andreas Kjaer</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown ON in LB with ampicillin and IPTG, refreshed in LB shortly prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-91/3dimage_73402/</p>10162015_TcpB_10091_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-91/3dimage_73402/10162015_TcpB_10091_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        10162015_TcpB_10091.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-91/rawdata/10162015_TcpB_10091.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p3yt7-ptv05
title: pED208_27.5k

description: F pilus and t4ss in E. coli
description: <strong>Tilt Series Date:</strong> 2015-01-18</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in LB o/n at 37C</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-18-12/rawdata/</p>pED208_275k0013.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-18-12/rawdata/pED208_275k0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED208_275k0013_part121_35.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-18-12/3dimage_50971/pED208_275k0013_part121_35.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j48j0-knw80
title: Mtr_recons_in_liposome

description: Final liposome conc: 2 mg/ml, grid # - PS123 - 2, ref pg 63 in notebook for details
description: <strong>Tilt Series Date:</strong> 2015-12-12</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 65.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh, USC</p>
<strong>Sample Preparation:</strong> vitrobot freezing conditions 6, 3- ref pg 63</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-15/3dimage_71426/</p>atlas1_sq40018_full.rec,  Reconstruction (Pixel Size 1.28 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-15/3dimage_71426/atlas1_sq40018_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas1_sq40018.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-12-12-15/rawdata/atlas1_sq40018.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r7zp1-wzk38
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-333/rawdata/</p>T4SSWTNov26A040_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-333/rawdata/T4SSWTNov26A040_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov26A040_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-333/3dimage_105078/T4SSWTNov26A040_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wwnkg-rs079
title: TM7 tomogram data

description: TM7  human oral cavity
description: <strong>Tilt Series Date:</strong> 2017-05-07</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Candidatus Saccharibacteria</p>
<strong>Strain:</strong> TM7</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-53.0°, 53.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Wenyuan Shi</p>
<strong>Sample Preparation:</strong> Force 6/ time 4/ duration 2  2 ul loading with 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-07-24/rawdata/</p>1tgt0024.mrc,  Tilt Series (Pixel Size 0.58 nm), 761.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-07-24/rawdata/1tgt0024.mrc"
        > <i class="download icon"></i> Download </a></p>

        1tgt0024_part107_2.rec,  Reconstruction (Pixel Size 2.32 nm), 78.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-05-07-24/3dimage_84161/1tgt0024_part107_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x4se6-can48
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-221/rawdata/</p>T4SSWTDec12A066_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-221/rawdata/T4SSWTDec12A066_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTDec12A066_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-221/3dimage_104985/T4SSWTDec12A066_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7nga5x
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is South America",
 "Country is Colombia",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Anolis gorgonae"
 ]
}

 The dataset includes 13 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047909-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bgmww-dka22
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-364/rawdata/</p>T4SSWTNov26A071_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-364/rawdata/T4SSWTNov26A071_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov26A071_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-364/3dimage_105106/T4SSWTNov26A071_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mx6vy-b4s17
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-53/rawdata/</p>LP02Jan30A006_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-53/rawdata/LP02Jan30A006_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        LP02Jan30A006_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-53/3dimage_104862/LP02Jan30A006_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d8smr-9t068
title: pED208_pilated

description: E coli strain with plasmid pED208 overexpresses T4SS and F-like pili
description: <strong>Tilt Series Date:</strong> 2015-01-14</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were obtained from Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> o/n liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-4/rawdata/</p>pED2080004.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-4/rawdata/pED2080004.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-4/3dimage_50586/pED2080004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pw9vt-emq05
title: #92+E.coli bamA purified

description: Raw data files of #92+E.coli bamA purified
description: <strong>Tilt Series Date:</strong> 2015-04-29</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chris Hayes and David Low UCSB</p>
<strong>Sample Preparation:</strong> These are E.cloacae mini cells (#92) mixed with purified E.coli BamA protein receptor. Both cells and BamA were blocked with BSA- 100g/ml for 30 minutes before mixing for 20 minutes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-10/rawdata/</p>PS96A-tomo0006.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-10/rawdata/PS96A-tomo0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        PS96A-tomo0006_full.rec,  Reconstruction (Pixel Size 1.32 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-10/3dimage_55176/PS96A-tomo0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tgyj4-sqg25
title: Coxiella burnetii_DeltaDotIcm_D5

description: Nine Mile phase II clone 4 (NMIIC4). Dot/Icm deletion strain, day-5 culture.
description: <strong>Tilt Series Date:</strong> 2017-01-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-14-18/rawdata/</p>CbDeltaDot_day5m0005.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-14-18/rawdata/CbDeltaDot_day5m0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        CbDeltaDot_day5m0005.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-14-18/3dimage_81119/CbDeltaDot_day5m0005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.huxdrp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Alectoris philbyi",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047910-250811113504898/datasets/export for details.
DOI: 10.22002/1mwtm-7d720
title: CLEM of TRIM5a-YFP in HeLa cells

description: HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS
description: <strong>Tilt Series Date:</strong> 2015-01-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 234.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-12/rawdata/</p>tomgraphytest21.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-12/rawdata/tomgraphytest21.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest21_part121_54.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-12/3dimage_51230/tomgraphytest21_part121_54.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sq9vh-dt350
title: Mycobacterium marinum

description: Grown on 2x LB to induce sporulation
description: <strong>Tilt Series Date:</strong> 2015-10-13</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Mycobacterium marinum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Will DePas from Dianne Newman's lab grew the cells</p>
<strong>Sample Preparation:</strong> Cells were frozen with Vitrobot Mark 4. Grids were positively glow discharged.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-13-5/3dimage_70177/</p>Mmarinum_oct20150035_part121_13.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
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        Mmarinum_oct20150035.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
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