DOI: 10.5061/dryad.r7nt4
title: Data from: Extensive trans-specific polymorphism at the mating type locus of the root decay fungus Heterobasidion

description: Incompatibility systems in which individuals bearing identical alleles
 reject each other favor the maintenance of a diversity of alleles.
 Mushroom mating type loci (MAT) encode for dozens or hundreds of
 incompatibility alleles whose loss from the population is greatly
 restricted through negative frequency selection, leading to a system of
 alleles with highly divergent sequences. Here we use DNA sequences of
 homeodomain (HD) encoding genes the MAT locus of five closely related
 species of the root rot basidiomycete Heterobasidion annosum sensu lato to
 show that the extended coalescence time of MAT alleles greatly predates
 speciation in the group, contrasting loci outside of MAT that show allele
 divergences largely consistent with the species phylogeny with those of
 MAT which show rampant trans-species polymorphism. We observe a roughly
 six-fold greater genealogical depth and polymorphism of MAT compared to
 non-MAT which argues for the maintenance of balanced polymorphism for a
 minimum duration of 24 million years based on a molecular-clock calibrated
 species phylogeny. As with other basidiomycete HD genes, balancing
 selection appears to be concentrated at the specificity-determining region
 in the N-terminus of the protein based on identification of codons under
 selection and the absence of recombination within the region. However, the
 elevated polymorphism extends into the non-specificity determining regions
 as well as a neighboring non-MAT gene, the mitochondrial intermediate
 peptidase (MIP). In doing so, increased divergence should decrease
 recombination among alleles and as a by-product create incompatibilities
 in the functional domains not involved in allele recognition but in
 regulating sexual development.
description: DNA sequence alignment
 of Ha-a1 geneAlignment of cDNA
 sequences of the Ha-a1 HD1 MAT gene, created in MacClade
 4.08.Ha-a1
 cds.nexDNA sequence alignment
 of Ha-a2 geneAlignment of cDNA
 sequences of the Ha-a1 HD2 MAT gene, created in MacClade
 4.08.Ha-a2
 cds.nexDNA sequence alignment
 of Ha-b1 geneAlignment of DNA
 sequences of the Ha-b1 HD1 MAT gene, created in MacClade
 4.08.Ha-b1
 cds.nexDNA sequence alignment
 of Ha-b2 geneAlignment of DNA
 sequences of the Ha-b2 HD2 MAT gene, created in MacClade
 4.08.Ha-b2
 cds.nexDNA sequence alignment
 of EFA geneAlignment of DNA
 sequences of the elongation factor 1-alpha gene, created in MacClade
 4.08.EFA_cds.nexDNA sequence alignment of G3P geneAlignment of coding DNA sequences of the elongation factor 1-alpha gene, created in MacClade 4.08.G3P_cds.nexDNA sequence alignment of Gst1 geneAlignment of coding DNA sequences of the glutathione-S-transferase 1 (GST1) gene, created in MacClade 4.08.Gst1_cds.nexDNA sequence alignment of TF geneAlignment of coding DNA sequences of the transcription factor gene (TF), created in MacClade 4.08.TF_gene.nexDNA sequence alignment of MIP geneAlignment of coding DNA sequences of the mitochondrial intermediate peptidase gene (MIP), created in MacClade 4.08.mip_cds.nex