DOI: 10.5061/dryad.7m0cfxq8k
title: Data from: Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides, and H2S in biological samples

description: This work describes the development and validation of a high-sensitivity
 analytical method (LC-MS/MS IAM-TPP) for the simultaneous quantification
 of protein persulfides, low molecular weight persulfides/polysulfides and
 hydrogen sulfide (H₂S) in complex biological matrices. The method was
 applied to biological samples to characterise H2S and endogenous
 persulfide/polysulfide formation, including analysis or physiologically
 relevant ischemia reperfusion models. The raw dataset is intended to
 enable independent re-analysis, method reuse, and further translational
 development in redox biology and drug discovery.
description: The data derive from experiments described in the manuscript:
 using the IAM-TPP technique, persulfide and polysulfide species were
 trapped, derivatised, separated by liquid chromatography, and quantified
 by tandem mass spectrometry. Sample preparation, calibration curves, and
 validation experiments (linearity, limit-of-detection, reproducibility)
 are detailed in the publication. Limitations: The
 dataset reflects specific sample types, instruments, and experimental
 conditions; normalisation and instrument-specific settings may differ in
 other laboratories. Raw data are provided to enable inspection of
 processing pipelines.
description: # Simultaneous and sensitive quantification of protein and low molecular
 weight persulfides, polysulfides, and H2S in biological samples Dataset
 DOI: [10.5061/dryad.7m0cfxq8k](https://doi.org/10.5061/dryad.7m0cfxq8k) ##
 Description of the data and file structure This dataset supports the
 publication: “Simultaneous and sensitive quantification of protein and low
 molecular weight persulfides, polysulfides and H₂S in biological samples”.
 ### Files and variables #### File: RAW_DATA.zip **Description:** **Files
 and variables** File: RAW_DATA.zip **Description: File structure and
 contents** RawData: Original instrument output files (LC-MS/MS raw data)
 corresponding to experiments presented in Figures 2, 4, 6, 5 and in
 Supplementary Figures 6, 7 and 8 of the main manuscript.   \_extern.info:
 contain metadata describing each sample, date, instrument run ID,
 biological replicate. ## Code/software **Code/software** No open-source or
 publicly available software was used for data generation or analysis. The
 raw LC–MS/MS data files can be opened and processed only using the Waters
 MassLynx software and associated QuanLynx processing module, which are
 proprietary to Waters Corporation. No additional scripts and/or custom
 code were used or are available beyond the manufacturer’s software
 environment. ## Access information Other publicly accessible locations of
 the data: All data associated with this study are original and generated
 by the authors. No data were obtained or derived from external sources.
 There are no additional publicly accessible datasets beyond those
 deposited in this Dryad repository and the supporting information
 available through the *Nature Communications* publication.