DOI: 10.5061/dryad.73n5tb35r
title: Aboveground fungal lethal effects of local plants on invasive plant Ageratina adenophora seedlings

description: We measured the effects of the aboveground and belowground microbes of 25
 native plant species and Ageratina adenophora on the germination and
 survival of invader A. adenophora in this study. We isolated fungi from
 dead seedlings 7 d after inoculated 0.1 g of plant tissue fragments with
 21-day-age seedlings (G21 experiment), and we tested the lethal effects of
 these strains on A. adenophora seedling survival. Some statistical
 analyses were performed in R. Thus, we provided two tables and R code
 files, including data of germination seedling survival.csv, lethal effect
 of 70 fungi on seedlings.txt, Script20250119. The file of data of
 germination seedling survival.csv provide the germination time, number of
 germinated seeds and survival seedling of G0 and G21 inoculation when
 inoculated aboveground tissue from 26 plant species, and the germination
 time, number of germinated seeds and survival seedling of G0 inoculation
 when inoculated soils from 26 plant species. The file of lethal effect of
 70 fungi on seedlings.txt provide the seedling mortality of A. adenophora
 caused by 70 fungal strains.
description: To quantify the effects of plant tissue and soil microbes of A.
 adenophora or different local native plant species on A. adenophora seed
 germination and seedling survival, we placed 16 surface-sterilized A.
 adenophora seeds on petri dishes and kept them as controls or inoculated
 them with 0.1 g of plant tissues or rhizosphere soil of a single species
 from a single site that were live or sterilized (hereafter referred to as
 G0 inoculation), resulting in a total of 525 plates (species [26] * soil
 vs.tissue [2] * sterile vs. unsterilized [2] * replicate [5] + control
 [5]). All petri dishes were randomly placed in a growth chamber (RXZ-380D,
 Ningbo Southeast Instrument Co., Ltd., Ningbo, China) and rearranged every
 week to mitigate potential positional effects. Petri dishes were kept at a
 temperature of 25/20°C (day/night), a light intensity of 12 000 lux (12h /
 12h) and a humidity of 65%. The number of germinated seeds was recorded
 for the first 14 days then again on the 21st day to calculate germination
 time (GT) and germination rate (GR). GT was calculated by the formula GT=
 Σ(Gi×i)/ΣGi (i: number of days between seed sowing (day 0) and seed
 germination; Gi: number of seeds germinated on day i). GR was calculated
 as the proportion of seeds germinated by the 21st day. The number of
 survival seedlings on the 28th day was used to calculate the seedling
 survival rate (named SSR_G0). Because we observed high seedling mortality
 with unsterilized tissue inoculation, we performed an additional
 inoculation on 21-day-old control seedlings to explore resistance of A.
 adenophora to plant tisses microbes across seedling ontogeny, resulting in
 a total of 265 plates (species [26] * sterile vs. unsterilized [2] *
 replicate [5] + control [5]). We used the proportion of seedlings alive 7
 days later to calculate SSR (hereafter referred to as SSR_G21).
 Furthermore, we tested the effect of 70 fungal strains on A.
 adenophora seedling survival. Sixteen surface-sterilized A. adenophora
 seeds were sown in a water agar petri dish. All 21-day-old seedlings in
 one petri dish were inoculated with one fungal strain by touching 3 mm
 diameter agar discs with fungal mycelia to the leaves and stems. Five
 petri dishes were used as five replicates for each strain. Fungi were
 grown on PDA for 7 days in an incubator at 25°C in the dark before
 inoculation. Seedlings were regarded as dead when the leaf and stem became
 brown and rotten. We calculated mortality rate as the proportion of
 seedlings that died in a dish 14 d after inoculation.
description: # **Aboveground fungal lethal effects of local plants on invasive plant
 *Ageratina adenophora* seedlings**
 [https://doi.org/10.5061/dryad.73n5tb35r](https://doi.org/10.5061/dryad.73n5tb35r) ## Description of the data and file structure * *data of germination seedling survival20241105.csv* file includes the effects of 26 plant leaf or soil inoculation on *Ageratina denophora* germination time, germination number and number of survival seedlings. * *lethal effect of 70 fungi on seedlings.txt*: file is the seedlings mortality causing by 70 fungal strains. * *Script20250119 .R* is code file used in this study, for example, Generalized linear models (GLMs), Nonmetric multidimensional scaling (NMDS) analysis, Mantel test, calculating phylogenetic signal (Pagel’s λ). ### Files and variables #### File: *data of germination seedling survival20241105*.csv **Description:** the effects of 26 plant leaf or soil inoculation on *Ageratina denophora* germination time, germination number, and number of survival seedlings. ##### Variables * Sample:  it is the 26 plant species with 5 replicates.  * Type: It includes leaves or soils. * Species: it provides the complete scientific names of  26 plant species. * Sterilization: It includes sterile or unsterilized. * germination time of G0: It is germination time of *Ageratina adenophora* inoculated plant tissues or soils at G0 inocualtion. Its units are day(d). * germination number of G0:  It is seedling numbers germinated of *Ageratina adenophora * after 21 days inoculated plant tissues or soils at G0 inoculation. * survival number of G0:  It is suvival seedling numbers of *Ageratina adenophora * after 28 days inoculated plant tissues or soils at G0 inoculation. * germination number of G21: It is seedling numbers germinated of *Ageratina adenophora * after 21 days without tissues or soils inoculated. * survival number of G21: It is suvival seedling numbers of *Ageratina adenophora * after 7 day inoculated plant tissues at G21 inocualtion. #### File: lethal\_effect\_of\_70\_fungi\_on\_seedlings.txt **Description:** The seedlings mortality caused by 70 fungal strains. ##### Variables * Strain code: It is the name of 70 fungi strains. * mortality 1: The proportion of dead seedlings to inoculated seedlings in a petri-dish as repetition one. * mortality 2:  The proportion of dead seedlings to inoculated seedlings in a petri-dish as repetition two. * mortality 3:  The proportion of dead seedlings to inoculated seedlings in a petri-dish as repetition three. * mortality 4:  The proportion of dead seedlings to inoculated seedlings in a petri-dish as repetition four. * mortality 5:  The proportion of dead seedlings to inoculated seedlings in a petri-dish as repetition five. * mean: The average of above five seedling mortality. * SEM: The Standard Error of above five seedling mortality. ## Code/software R 4.3.3, Gephi 0.10.1 were required. R Code is included in our submission, including analysis of Generalized linear models (GLMs), Nonmetric multidimensional scaling (NMDS), Mantel test, phylogenetic signal (Pagel’s λ). ## Access information Other publicly accessible locations of the data: * No. Data was derived from the following sources: * No.