cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7773956
title: Identification of novel mutations of Insulin Receptor Substrate 1 (IRS1) in tumor samples of non-small cell lung cancer (NSCLC): Implications for aberrant insulin signaling in development of cancer

description: Abstract Lung cancer is the leading cause of cancer-related death, and NSCLC constitutes nearly 85%–90% of all cases. The IRS proteins function as adaptors and transmit signals from multiple receptors. Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. Therefore, we thought that gain of function mutantions of IRS1 could be related to development of lung cancer. In line with this, we wanted determine whether the IRS1 gene was mutated in the coding regions surrounding YXXM motifs. We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote p.S668T mutation in nine of 42 samples and four of nine also had the p.D674H mutation. We generated IRS1 expression vectors harboring p.S668T, p.D674H and double mutants. Expression of the mutants differentially affected insulin-induced phosphorylation of IRS1, AKT, ERK, and STAT3. Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. Our results suggest that these novel mutations play a role in the phenotype of lung cancer.
DOI: 10.6084/m9.figshare.7773956.v1
title: Identification of novel mutations of Insulin Receptor Substrate 1 (IRS1) in tumor samples of non-small cell lung cancer (NSCLC): Implications for aberrant insulin signaling in development of cancer

description: Abstract Lung cancer is the leading cause of cancer-related death, and NSCLC constitutes nearly 85%–90% of all cases. The IRS proteins function as adaptors and transmit signals from multiple receptors. Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. Therefore, we thought that gain of function mutantions of IRS1 could be related to development of lung cancer. In line with this, we wanted determine whether the IRS1 gene was mutated in the coding regions surrounding YXXM motifs. We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote p.S668T mutation in nine of 42 samples and four of nine also had the p.D674H mutation. We generated IRS1 expression vectors harboring p.S668T, p.D674H and double mutants. Expression of the mutants differentially affected insulin-induced phosphorylation of IRS1, AKT, ERK, and STAT3. Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. Our results suggest that these novel mutations play a role in the phenotype of lung cancer.
DOI: 10.6084/m9.figshare.7773947
title: EFFECT OF NOZZLES, APPLICATION RATES, AND ADJUVANTS ON SPRAY DEPOSITION IN WHEAT CROPS

description: ABSTRACT The use of insecticides is common in pest management in wheat crops but may lead to higher production costs and environmental contamination. Therefore, a more comprehensive and uniform distribution of plant protection products in the desired target is recommended. The objective of this study is to evaluate the effect of different spray nozzles, application rates, and adjuvants on droplet spectrum and spray deposition in wheat crops. The physicochemical characteristics of the spray mixture, droplet spectrum, and spray deposition in wheat plants were evaluated. The lower application rate (75 L ha−1) combined with the use of the twin flat fan tip AS7030 and the adjuvant sodium lauryl ether sulfate (SLES) yielded droplets with a larger diameter. However, insecticide spraying using hollow cone tip MGA 015 without adjuvants provided the highest percentage of droplets susceptible to drift. SLES significantly decreased the percentage of drift by increasing droplet size, and the adjuvant silicone, d-limonene, and polyester copolymer increased spray deposition. The evaluated adjuvants increased the electrical conductivity and reduced the surface tension of the spray mixtures. These results indicate that it is possible to reduce the application rate from 150 to 75 L ha−1 without affecting spray deposition on wheat leaves.
DOI: 10.6084/m9.figshare.7773941
title: Effect of active antimicrobial films on the growth of pathogenic bacteria in Mantı

description: Abstract The current study assessed antimicrobial films for use in active food packaging. Mantı, a tortellini-like Turkish food, was packaged with antimicrobial films to extend refrigerated storage time at 4 °C. Polycaprolactone (PCL) was separately incorporated with cinnamaldehyde (CNMA) and pomegranate methanolic extract (PME) to form antimicrobial diffusion films for use against two common food-poisoning bacteria, Escherichia coli and Staphylococcus aureus, and the mantı pieces were separately inoculated with 5 log concentrations of both types of bacteria. The films and mantı were then inserted in polyethylene bags, which were filled with 100% N2 gas and monitored at 4 °C for 28 days. The results clearly show that both a low temperature storage (4 °C) and antimicrobial films controlled microbial growth in packages of mantı kept in a modified atmosphere (100% N2) for a 28-day period. The findings are promising from an industrial standpoint with regard to bakery foods that are packaged and preserved for short-term periods (&lt;30 days).
DOI: 10.6084/m9.figshare.7773947.v1
title: EFFECT OF NOZZLES, APPLICATION RATES, AND ADJUVANTS ON SPRAY DEPOSITION IN WHEAT CROPS

description: ABSTRACT The use of insecticides is common in pest management in wheat crops but may lead to higher production costs and environmental contamination. Therefore, a more comprehensive and uniform distribution of plant protection products in the desired target is recommended. The objective of this study is to evaluate the effect of different spray nozzles, application rates, and adjuvants on droplet spectrum and spray deposition in wheat crops. The physicochemical characteristics of the spray mixture, droplet spectrum, and spray deposition in wheat plants were evaluated. The lower application rate (75 L ha−1) combined with the use of the twin flat fan tip AS7030 and the adjuvant sodium lauryl ether sulfate (SLES) yielded droplets with a larger diameter. However, insecticide spraying using hollow cone tip MGA 015 without adjuvants provided the highest percentage of droplets susceptible to drift. SLES significantly decreased the percentage of drift by increasing droplet size, and the adjuvant silicone, d-limonene, and polyester copolymer increased spray deposition. The evaluated adjuvants increased the electrical conductivity and reduced the surface tension of the spray mixtures. These results indicate that it is possible to reduce the application rate from 150 to 75 L ha−1 without affecting spray deposition on wheat leaves.
DOI: 10.6084/m9.figshare.7773935
title: THEORETICAL STUDIES ON TRIACETONETRIPEROXIDE (TATP) DERIVATIVES TO IMPROVE THEIR PERFORMANCE

description: Density functional theory (DFT) methods are used to study on TATP derivatives at DFT-B3LYP/6-311++G (d, p) level to improve TATP’s performance. The derivatives are 3,6,9-trimethyl-3,6,9-tris(trifluoromethyl)-1,2,4,5,7,8-hexaoxonane (TATP3F), 3,6,9-trimethyl-3,6,9-tris(nitromethyl)-1,2,4,5,7,8-hexaoxonane (TATPNO2) and (3,6,9-trimethyl-1,2,4,5,7,8-hexaoxonane-3,6,9-triyl)trimethanamine (TATPNH2). Stability and detonation performance of TATP derivatives were investigated. Detonation performance was estimated using density, heat of formation and EXPLO 5 software. Stability was evaluated by geometrical geometry and electronic structure. The simulation results reveal that TATPNO2 has a best oxygen balance, detonation performance (Q=5424 kJ kg-1, P=23.7 Gpa, D=7699 m s-1) and insensitivity. Since TATPNO2 is more excellent than TATP and traditional explosive TNT in detonation performance, it is a candidate of novel high energy density materials.
DOI: 10.6084/m9.figshare.7773938
title: Risk Factors and Outcome of Acute Kidney Injury after Isolated CABG Surgery: a Prospective Cohort Study

description: Abstract Background: Acute kidney injury (AKI) is a frequent event after cardiac surgery with increased mortality and morbidity. We explored frequency, risk factors, and associated morbidity and mortality of AKI after isolated coronary artery bypass grafting (CABG) surgery at a single institution. Methods: All consecutive adults undergoing CABG surgery from March 2013 to October 2016 were assessed for development and severity of AKI based on Acute Kidney Injury Network (AKIN) criteria. The patients were also investigated regarding their need for renal replacement therapy (RRT), predictive risk factors, and associated outcomes, including duration of mechanical ventilation, mortality, intensive care unit (ICU) and hospital length of stay. Results: Of 1737 patients in the study, 275 (15.8%) developed AKI. Twenty-five (12.8%) cases required RRT. Patients with AKI had longer ventilation time, ICU and hospital length of stay (P&lt;0.001). Mortality rates were 28 (10.2%) and 22 (1.5%) in patients with and without AKI, respectively (P&lt;0.001). There was a strong association between advanced age (aOR=1.016, 95% CI=1.002-1.030, P=0.028), diabetes (aOR=1.36, 95% CI=1.022-1.809, P=0.035), on-pump surgery (aOR=2.63, 95% CI=1.543-4.483, P&lt;0.001), transfusion of more than 1 unit of red blood cells (aOR=2.154, 95% CI=1.237-3.753, P=0.007), and prolonged mechanical ventilation and development of AKI (aOR=2.697, 95% CI=1.02407.071, P&lt;0.001). AKI was seen less frequently in those with opium abuse (aOR=0.613, 95% CI=0.409-0.921, P=0.018). Conclusion: We demonstrated that advanced age, diabetes, on-pump surgery, red blood cell transfusion, and prolonged mechanical ventilation were independent positive risk factors for the development of AKI after isolated CABG while opium abuse was a protective factor.
DOI: 10.6084/m9.figshare.7773941.v1
title: Effect of active antimicrobial films on the growth of pathogenic bacteria in Mantı

description: Abstract The current study assessed antimicrobial films for use in active food packaging. Mantı, a tortellini-like Turkish food, was packaged with antimicrobial films to extend refrigerated storage time at 4 °C. Polycaprolactone (PCL) was separately incorporated with cinnamaldehyde (CNMA) and pomegranate methanolic extract (PME) to form antimicrobial diffusion films for use against two common food-poisoning bacteria, Escherichia coli and Staphylococcus aureus, and the mantı pieces were separately inoculated with 5 log concentrations of both types of bacteria. The films and mantı were then inserted in polyethylene bags, which were filled with 100% N2 gas and monitored at 4 °C for 28 days. The results clearly show that both a low temperature storage (4 °C) and antimicrobial films controlled microbial growth in packages of mantı kept in a modified atmosphere (100% N2) for a 28-day period. The findings are promising from an industrial standpoint with regard to bakery foods that are packaged and preserved for short-term periods (&lt;30 days).
DOI: 10.6084/m9.figshare.7773932
title: TRACTIVE DEMAND, SOIL MOBILIZATION PARAMETERS, AND CORN YIELD IN COMPACTED OXISOL

description: ABSTRACT Direct seeding systems are characterized by soil disturbance only occurring in the sowing line and the maintenance of the remaining area is achieved by covering with crop residues. Without total area soil mobilization as recommended by the no-tillage system, associated with the increase in the degree of soil compaction caused by the traffic of agricultural machinery, there can be a decrease in crop productivity. This study aimed to evaluate machine parameters, soil mobilization, and corn yield as a function of different furrow opener working depths (0.065, 0.095, 0.125, and 0.150 m) and evaluate the direct seeding of winter ground cover plants (black oat or forage turnip) in compacted Oxisol. The experiment was arranged in strips, in a randomized block design, with eight treatments and four replications. The deepening of the furrow opener shank increased the strength and power required at the drawbar in the seeding operation, as well as increased the soil mobilization in the seeding line. However, it did not prove to be an efficient management practice to increase corn productivity.
DOI: 10.6084/m9.figshare.7773938.v1
title: Risk Factors and Outcome of Acute Kidney Injury after Isolated CABG Surgery: a Prospective Cohort Study

description: Abstract Background: Acute kidney injury (AKI) is a frequent event after cardiac surgery with increased mortality and morbidity. We explored frequency, risk factors, and associated morbidity and mortality of AKI after isolated coronary artery bypass grafting (CABG) surgery at a single institution. Methods: All consecutive adults undergoing CABG surgery from March 2013 to October 2016 were assessed for development and severity of AKI based on Acute Kidney Injury Network (AKIN) criteria. The patients were also investigated regarding their need for renal replacement therapy (RRT), predictive risk factors, and associated outcomes, including duration of mechanical ventilation, mortality, intensive care unit (ICU) and hospital length of stay. Results: Of 1737 patients in the study, 275 (15.8%) developed AKI. Twenty-five (12.8%) cases required RRT. Patients with AKI had longer ventilation time, ICU and hospital length of stay (P&lt;0.001). Mortality rates were 28 (10.2%) and 22 (1.5%) in patients with and without AKI, respectively (P&lt;0.001). There was a strong association between advanced age (aOR=1.016, 95% CI=1.002-1.030, P=0.028), diabetes (aOR=1.36, 95% CI=1.022-1.809, P=0.035), on-pump surgery (aOR=2.63, 95% CI=1.543-4.483, P&lt;0.001), transfusion of more than 1 unit of red blood cells (aOR=2.154, 95% CI=1.237-3.753, P=0.007), and prolonged mechanical ventilation and development of AKI (aOR=2.697, 95% CI=1.02407.071, P&lt;0.001). AKI was seen less frequently in those with opium abuse (aOR=0.613, 95% CI=0.409-0.921, P=0.018). Conclusion: We demonstrated that advanced age, diabetes, on-pump surgery, red blood cell transfusion, and prolonged mechanical ventilation were independent positive risk factors for the development of AKI after isolated CABG while opium abuse was a protective factor.
DOI: 10.6084/m9.figshare.7773926
title: Agreement between diagnostic imaging methods for the evaluation of lymphadenopathies in HIV-infected/AIDS patients

description: Abstract Objective: To assess the percent agreement between diagnostic imaging modalities for the evaluation of lymphadenopathies in HIV-infected/AIDS patients. Materials and Methods: This was an open, comparative, prospective study of diagnostic imaging methods for lymphadenopathy evaluation. We evaluated 30 patients (19 men and 11 women). All underwent ultrasound and computed tomography (CT). Twenty of the patients also underwent magnetic resonance imaging (MRI). We determined the percent agreement between two examiners using the various imaging methods to evaluate lymphadenopathies. Results: CT had the highest percent agreement, at 93.3%, with a kappa coefficient of 0.85, corresponding to 28 of the 30 examinations. When we compared the percent agreement between the two examiners and between CT and ultrasound, examiner 1 had an observed rate of 80.0%, with a kappa of 0.49, corresponding to 24 of the 30 examinations, whereas examiner 2 had a rate of 70.0%, with a kappa of 0.31, corresponding to 21 of the 30 examinations. Between MRI and CT, the percent agreement for examiner 1 was 50.0%, with a kappa of −0.18, corresponding to 10 of the 20 examinations, whereas that for examiner 2 was 85.0%, with a kappa of 0.69, corresponding to 17 of the 20 examinations. For MRI and ultrasound, examiner 1 had a percent agreement of 70.0%, with a kappa of 0.20, corresponding to 14 of the 20 examinations, and examiner 2 had a percent agreement of 75.0%, with a kappa of 0.38, corresponding to 15 of the 20 examinations. Conclusion: This study indicates that intermethod agreement is highly dependent on the way in which the research is conducted, rather than on the level of experience of the examiner.
DOI: 10.6084/m9.figshare.7773932.v1
title: TRACTIVE DEMAND, SOIL MOBILIZATION PARAMETERS, AND CORN YIELD IN COMPACTED OXISOL

description: ABSTRACT Direct seeding systems are characterized by soil disturbance only occurring in the sowing line and the maintenance of the remaining area is achieved by covering with crop residues. Without total area soil mobilization as recommended by the no-tillage system, associated with the increase in the degree of soil compaction caused by the traffic of agricultural machinery, there can be a decrease in crop productivity. This study aimed to evaluate machine parameters, soil mobilization, and corn yield as a function of different furrow opener working depths (0.065, 0.095, 0.125, and 0.150 m) and evaluate the direct seeding of winter ground cover plants (black oat or forage turnip) in compacted Oxisol. The experiment was arranged in strips, in a randomized block design, with eight treatments and four replications. The deepening of the furrow opener shank increased the strength and power required at the drawbar in the seeding operation, as well as increased the soil mobilization in the seeding line. However, it did not prove to be an efficient management practice to increase corn productivity.
DOI: 10.6084/m9.figshare.7773935.v1
title: THEORETICAL STUDIES ON TRIACETONETRIPEROXIDE (TATP) DERIVATIVES TO IMPROVE THEIR PERFORMANCE

description: Density functional theory (DFT) methods are used to study on TATP derivatives at DFT-B3LYP/6-311++G (d, p) level to improve TATP’s performance. The derivatives are 3,6,9-trimethyl-3,6,9-tris(trifluoromethyl)-1,2,4,5,7,8-hexaoxonane (TATP3F), 3,6,9-trimethyl-3,6,9-tris(nitromethyl)-1,2,4,5,7,8-hexaoxonane (TATPNO2) and (3,6,9-trimethyl-1,2,4,5,7,8-hexaoxonane-3,6,9-triyl)trimethanamine (TATPNH2). Stability and detonation performance of TATP derivatives were investigated. Detonation performance was estimated using density, heat of formation and EXPLO 5 software. Stability was evaluated by geometrical geometry and electronic structure. The simulation results reveal that TATPNO2 has a best oxygen balance, detonation performance (Q=5424 kJ kg-1, P=23.7 Gpa, D=7699 m s-1) and insensitivity. Since TATPNO2 is more excellent than TATP and traditional explosive TNT in detonation performance, it is a candidate of novel high energy density materials.
DOI: 10.6084/m9.figshare.7773920
title: Quantification of visceral adipose tissue by computed tomography and magnetic resonance imaging: reproducibility and accuracy

description: Abstract Objective: To evaluate the feasibility of quantifying visceral adipose tissue (VAT) on computed tomography (CT) and magnetic resonance imaging (MRI) scans, using freeware, as well as calculating intraobserver and interobserver reproducibility. Materials and Methods: We quantified VAT in patients who underwent abdominal CT and MRI at our institution between 2010 and 2015, with a maximum of three months between the two examinations. A slice acquired at the level of the umbilicus was selected. Segmentation was performed with the region growing algorithm of the freeware employed. Intraobserver and interobserver reproducibility were evaluated, as was the accuracy of MRI in relation to that of CT. Results: Thirty-one patients (14 males and 17 females; mean age of 57 ± 15 years) underwent CT and MRI (mean interval between the examinations, 28 ± 12 days). The interobserver reproducibility was 82% for CT (bias = 1.52 cm2; p = 0.488), 86% for T1-weighted MRI (bias = −4.36 cm2; p = 0.006), and 88% for T2-weighted MRI (bias = −0.52 cm2; p = 0.735). The intraobserver reproducibility was 90% for CT (bias = 0.14 cm2; p = 0.912), 92% for T1-weighted MRI (bias = −3,4 cm2; p = 0.035), and 90% for T2-weighted MRI (bias = −0.30 cm2; p = 0.887). The reproducibility between T1-weighted MRI and T2-weighted MRI was 87% (bias = −0.11 cm2; p = 0.957). In comparison with the accuracy of CT, that of T1-weighted and T2-weighted MRI was 89% and 91%, respectively. Conclusion: The program employed can be used in order to quantify VAT on CT, T1-weighted MRI, and T2-weighted MRI scans. Overall, the accuracy of MRI (in comparison with that of CT) appears to be high, as do intraobserver and interobserver reproducibility. However, the quantification of VAT seems to be less reproducible in T1-weighted sequences.
DOI: 10.6084/m9.figshare.7773926.v1
title: Agreement between diagnostic imaging methods for the evaluation of lymphadenopathies in HIV-infected/AIDS patients

description: Abstract Objective: To assess the percent agreement between diagnostic imaging modalities for the evaluation of lymphadenopathies in HIV-infected/AIDS patients. Materials and Methods: This was an open, comparative, prospective study of diagnostic imaging methods for lymphadenopathy evaluation. We evaluated 30 patients (19 men and 11 women). All underwent ultrasound and computed tomography (CT). Twenty of the patients also underwent magnetic resonance imaging (MRI). We determined the percent agreement between two examiners using the various imaging methods to evaluate lymphadenopathies. Results: CT had the highest percent agreement, at 93.3%, with a kappa coefficient of 0.85, corresponding to 28 of the 30 examinations. When we compared the percent agreement between the two examiners and between CT and ultrasound, examiner 1 had an observed rate of 80.0%, with a kappa of 0.49, corresponding to 24 of the 30 examinations, whereas examiner 2 had a rate of 70.0%, with a kappa of 0.31, corresponding to 21 of the 30 examinations. Between MRI and CT, the percent agreement for examiner 1 was 50.0%, with a kappa of −0.18, corresponding to 10 of the 20 examinations, whereas that for examiner 2 was 85.0%, with a kappa of 0.69, corresponding to 17 of the 20 examinations. For MRI and ultrasound, examiner 1 had a percent agreement of 70.0%, with a kappa of 0.20, corresponding to 14 of the 20 examinations, and examiner 2 had a percent agreement of 75.0%, with a kappa of 0.38, corresponding to 15 of the 20 examinations. Conclusion: This study indicates that intermethod agreement is highly dependent on the way in which the research is conducted, rather than on the level of experience of the examiner.