DOI: 10.5061/dryad.rr4xgxdmr
title: Effect of diapause duration on thermal tolerance in the cabbage beetle <em>Colaphellus bowringi</em> Baly

description: This dataset comprises the raw experimental data supporting the findings
 of the study "Effect of Diapause Duration on Thermal Tolerance in the
 Cabbage Beetle Colaphellus bowringi Baly." The data were collected to
 investigate how different diapause durations (0.5, 1, 1.5, and 2 years)
 affect heat tolerance in both parental adults and their offspring across
 multiple life stages.
description: # Effect of diapause duration on thermal tolerance in the cabbage beetle
 *Colaphellus bowringi Baly* Dataset DOI:
 [10.5061/dryad.rr4xgxdmr](https://doi.org/10.5061/dryad.rr4xgxdmr) ##
 Description of the data and file structure This dataset comprises the raw
 experimental data supporting the findings of the study "Effect of
 Diapause Duration on Thermal Tolerance in the Cabbage Beetle Colaphellus
 bowringi Baly." The data were collected to investigate how different
 diapause durations (0.5, 1, 1.5, and 2 years) affect heat tolerance in
 both parental adults and their offspring across multiple life stages. The
 experimental efforts included: /1. Parental Adult Survival Assays:
 Post-diapause adults from four diapause duration groups were exposed to
 acute heat stress at temperatures of 30°C, 33°C, 36°C, and 39°C for
 durations of 1, 3, 6, 12, and 24 hours. Survival rates were recorded
 immediately after each exposure period. /2. Offspring Thermal Tolerance
 Assessments: F1 generation individuals (eggs, larvae, pupae, and adults)
 derived from parents of all four diapause durations were subjected to heat
 stress: Eggs: 20°C, 27°C, 30°C, 33°C, and 36°C for 24 hours Larvae, Pupae,
 and Adults: 20°C, 36°C, 39°C, 42°C, and 45°C for 24 hours Survival metrics
 included hatching rates (eggs), survival rates (larvae/adults), and
 emergence rates (pupae) /3. Respiratory Metabolism Measurements: F1
 generation 7-day-old larvae from all parental diapause groups were exposed
 to 20°C, 36°C, 39°C, 42°C, and 45°C for 6 hours. Individual larval CO₂
 emission rates were measured using a multi-channel insect respiratory
 measurement system, with respiration rates calculated accounting for
 chamber volume, temperature, and body weight. ### Files and variables ####
 File: Raw_Data_(Parental_adult_survival).xlsx **Description:** This file
 contains survival data of post-diapause adult beetles exposed to high
 temperatures (30 °C, 33 °C, 36 °C, 39 °C) for varying durations (1–24
 hours), grouped by diapause duration (0.5, 1, 1.5, 2 years).Sheets:
 **Sheet1:** Survival data for each temperature and exposure time.
 **Variables:** Exposure Temperature (℃): Temperature at which adults were
 exposed (20, 30, 33, 36, 39°C). Exposure Time (h): Duration of exposure
 (1, 3, 6, 12, 24 hours). Diapause Duration (0.5yrs / 1yr / 1.5yrs / 2yrs):
 Duration of diapause for each experimental group. Total (individuals):
 Number of individuals exposed per replicate. Survived (individuals):
 Number of individuals surviving after exposure. Survival Rate (%):
 Percentage of survivors per replicate. #### File:
 Raw_Data_(offspring_survival).xlsx **Description:** This file contains
 survival data of offspring (F1 generation) from parents with different
 diapause durations, exposed to high temperatures at four life stages: egg,
 larva, pupa, and adult. ##### Sheets: Sheet1: Survival data for each life
 stage and temperature. **Variables:** Exposure Stage: Life stage of
 offspring (egg, larva, pupa, adult). Exposure Temperature (℃): Temperature
 at which offspring were exposed (eggs: 20–36°C; other stages: 20–45°C).
 Diapause Duration (0.5yrs / 1yr / 1.5yrs / 2yrs): Parental diapause
 duration. Total (eggs/individuals): Number of eggs or individuals exposed
 per replicate. Hatched / Survived (individuals): Number of hatched eggs or
 surviving individuals. Hatching Rate / Survival Rate (%): Percentage of
 hatched or surviving individuals. **Life Stages & Test Conditions:**
 Eggs: 20–36 °C, 24 h exposure Larvae: 20–45 °C, 24 h exposure Pupae:
 20–45 °C, 24 h exposure Adults: 20–45 °C, 24 h exposure #### File:
 Raw_Data_(offspring_respiration_rate).xlsx **Description:** This file
 contains respiration rate data of F1 larvae (7-day-old) exposed to high
 temperatures (20 °C, 36 °C, 39 °C, 42 °C, 45 °C) for 6 hours, measured
 using a CO₂ respirometry system. **Sheets:** Sheet1: Respiration
 measurements for each temperature and diapause group. **Variables:**
 Exposure Temperature (℃): Temperature at which larvae were exposed (20,
 36, 39, 42, 45°C). Diapause Duration (0.5yrs / 1yr / 1.5yrs / 2yrs):
 Parental diapause duration. Respiration chamber CO₂ reading: CO₂
 concentration at the end of measurement (ppm or μL/L). Respiration chamber
 CO₂ baseline value: Initial CO₂ concentration in the chamber. Insect body
 weight (g): Weight of individual larva. Respiration Rate (μmol
 CO₂·g⁻¹·s⁻¹): Respiration rate per second. Respiration Rate (μmol
 CO₂·g⁻¹·h⁻¹): Respiration rate per hour. ## Code/software **1. Software
 Requirements for Viewing Data:** The raw data files are provided in
 **Microsoft Excel (.xlsx) format**. To view and analyze these files, the
 following software (or equivalent) is recommended: * **Microsoft Excel**
 (version 2016 or later) * **LibreOffice Calc** (version 7.0 or later) *
 **Google Sheets** (via upload to Google Drive) No specialized packages or
 plugins are required to view the data. --- **2. Software Used for Data
 Analysis in the Study:** All statistical analyses and data processing
 reported in the associated manuscript were performed using: * **IBM SPSS
 Statistics** (Version 31.0) Specific procedures included: * **Probit
 Analysis** for estimating LT₅₀ (median lethal temperature) and
 corresponding confidence intervals. * **One-way Analysis of Variance
 (ANOVA)** to compare survival rates and respiratory rates across
 treatments. * **Tukey’s Honestly Significant Difference (HSD) Test** for
 post-hoc multiple comparisons. No custom scripts or code were used; all
 analyses were conducted using the built-in functions of SPSS. --- **3.
 Data Workflow Description:** The relationship between the raw data files
 and the statistical analyses is as follows: * **"Raw Data (Parental
 adult survival).xlsx"** → Used for analyzing the effect of diapause
 duration on adult heat tolerance. → Data were transformed to Probit units
 for LT₅₀ estimation in SPSS. * **"Raw Data (offspring
 survival).xlsx"** → Contains survival/hatch/emergence rates for
 offspring across life stages (eggs, larvae, pupae, adults). → Used in
 Section 3.2 to assess transgenerational effects of diapause duration. *
 **"Raw Data (offspring respiration rate).xlsx"** → Provides
 respiratory metabolism data for larvae under heat stress. → Used in
 Section 3.3 to generate thermal performance curves and compare respiration
 rates across temperatures. All data were first organized in Excel, then
 imported into SPSS for statistical testing. Figures and tables in the
 manuscript were generated based on the output from these analyses. ##
 Access information Other publicly accessible locations of the data: * None
 Data was derived from the following sources: * None