cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8091845.v1
title: Egg quality of laying hens fed diets with plant extracts

description: ABSTRACT. The objective of this study was to evaluate the effect of marigold flower extract and paprika on egg quality of Black Avifran laying hens in different periods of storage. A completely randomized design study with a 3x3 factorial scheme with three diets (control, paprika, and marigold flower extract) and three storage periods (0, 7, and 14 days) was carried out. The following metrics were evaluated: egg weight, yolk weight, shell weight; yolk color, albumen height, specific gravity, and Haugh unit as well as the yolk, shell, and albumen percentage relative to the total weight of the egg. There was no significant interaction between additives and storage time (p &gt; 0.05) for most of the parameters evaluated. The only exception was observed for yolk color, in which the interaction effect between treatments was significant (p &lt; 0.05). The effect of storage time was significant (p &lt; 0.05) for albumen weight, albumen percentage, yolk color, albumen height, specific gravity, and Haugh unit, with reduction of these parameters over the evaluation period. The use of paprika and marigold flower extract in the diets of Black Avifran laying hens does not influence egg quality.
DOI: 10.6084/m9.figshare.8091833
title: Metabolizable energy values of soybean meals and soybean oil for broilers at different ages

description: ABSTRACT. One trial with six analyses of different diets at different ages was carried out to determine the values of apparent (AME), corrected (AMEn), true (TME) and true corrected (TMEn) metabolizable energy of SBM and SBO for broiler chickens from 1 to 42 days of age. Three Brazilian SBM samples and one SBO were evaluated by replacing part of the basal diet with the test ingredient (300 g kg-1 for SBM and 100 g kg-1 for SBO). A total of 1.368 one-day-old male Ross 308 birds were assigned into 36 metabolic cages according to weight in a completely randomized experimental design with six replicates for each test ingredient and basal diet. Six more replicates of fasted birds were used for the determination of metabolic energy and N losses at each age. Four days of adaptation followed by three days of excreta collection for energy measurement. The birds were reared until each age, in solid floor pens with a corn/soybean basal diet. The total excreta collection method was used. The AME, AMEn, TME and TMEn of SBM and SBO increased (p &lt; 0.05) until 28 days and after this time no difference (p &gt;0.05) was observed.
DOI: 10.6084/m9.figshare.8091833.v1
title: Metabolizable energy values of soybean meals and soybean oil for broilers at different ages

description: ABSTRACT. One trial with six analyses of different diets at different ages was carried out to determine the values of apparent (AME), corrected (AMEn), true (TME) and true corrected (TMEn) metabolizable energy of SBM and SBO for broiler chickens from 1 to 42 days of age. Three Brazilian SBM samples and one SBO were evaluated by replacing part of the basal diet with the test ingredient (300 g kg-1 for SBM and 100 g kg-1 for SBO). A total of 1.368 one-day-old male Ross 308 birds were assigned into 36 metabolic cages according to weight in a completely randomized experimental design with six replicates for each test ingredient and basal diet. Six more replicates of fasted birds were used for the determination of metabolic energy and N losses at each age. Four days of adaptation followed by three days of excreta collection for energy measurement. The birds were reared until each age, in solid floor pens with a corn/soybean basal diet. The total excreta collection method was used. The AME, AMEn, TME and TMEn of SBM and SBO increased (p &lt; 0.05) until 28 days and after this time no difference (p &gt;0.05) was observed.
DOI: 10.6084/m9.figshare.8091842
title: Effect of enzyme addition on energy utilization and performance of broiler chickens fed wheat-based diet with different metabolizable energy levels

description: ABSTRACT. An experiment was carried out to investigate the effect of multi-enzyme in high and low levels of metabolizable energy (13.81 and 11.51 MJ kg-1 diet) on performance and energy utilization of broilers fed wheat-soybean meal diets from 0 to 21 days of age. Result showed that birds fed diets containing 11.51 MJ kg-1 consumed significantly (p &lt; 0.05) more feed than diets containing 13.81 MJ kg-1, whereas daily gain and feed conversion ratio improved (p &lt; 0.05) when enzyme was added to 11.51 MJ kg-1 diet. There was significant improvement in metabolizable energy, net energy for production, organic and dry matter digestibility in 0-21 when diets supplemented with enzyme (p &lt; 0.05). Addition of enzyme to 11.51 MJ kg-1 containing diet significantly (p &lt; 0.05) reduced heat production of birds in 0-10 d, whereas heat production was not changed in 21 days. Supplementation of 11.51 MJ kg-1 diet with enzyme improved the efficiency of ME use for carcass energy and protein retention of broilers (p &lt; 0.05). Generally, the results of current study demonstrated that addition of enzyme to wheat-soybean diets improved NEp of broiler chickens while MEI was not changed and it seems NEp is a more sensitive energy utilization response criterion to use in evaluating broilers response to enzyme supplementation.
DOI: 10.6084/m9.figshare.8091842.v1
title: Effect of enzyme addition on energy utilization and performance of broiler chickens fed wheat-based diet with different metabolizable energy levels

description: ABSTRACT. An experiment was carried out to investigate the effect of multi-enzyme in high and low levels of metabolizable energy (13.81 and 11.51 MJ kg-1 diet) on performance and energy utilization of broilers fed wheat-soybean meal diets from 0 to 21 days of age. Result showed that birds fed diets containing 11.51 MJ kg-1 consumed significantly (p &lt; 0.05) more feed than diets containing 13.81 MJ kg-1, whereas daily gain and feed conversion ratio improved (p &lt; 0.05) when enzyme was added to 11.51 MJ kg-1 diet. There was significant improvement in metabolizable energy, net energy for production, organic and dry matter digestibility in 0-21 when diets supplemented with enzyme (p &lt; 0.05). Addition of enzyme to 11.51 MJ kg-1 containing diet significantly (p &lt; 0.05) reduced heat production of birds in 0-10 d, whereas heat production was not changed in 21 days. Supplementation of 11.51 MJ kg-1 diet with enzyme improved the efficiency of ME use for carcass energy and protein retention of broilers (p &lt; 0.05). Generally, the results of current study demonstrated that addition of enzyme to wheat-soybean diets improved NEp of broiler chickens while MEI was not changed and it seems NEp is a more sensitive energy utilization response criterion to use in evaluating broilers response to enzyme supplementation.
DOI: 10.6084/m9.figshare.20013263
title: Supplementary Material for: Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery

description: Idiopathic pulmonary fibrosis (IPF) is a type of idiopathic interstitial pneumonia with a poor clinical prognosis. Increasing evidence has demonstrated that epithelial-mesenchymal transition (EMT) contributes to the production of pathogenic myofibroblasts and plays a pivotal role in the development of pulmonary fibrosis. Mannan-binding lectin (MBL) is a soluble calcium-dependent complement molecule. Several studies have reported associations between serum MBL levels and lung diseases; however, the effect of MBL on IPF remains unknown. The present study observed aggravated pulmonary fibrosis in bleomycin-treated MBL<sup>−/−</sup> mice compared with their wild-type counterparts. Lung tissues from bleomycin-treated MBL<sup>−/−</sup> mice displayed a more severe EMT phenotype. In vitro studies determined that MBL inhibited the EMT process through attenuating store-operated calcium entry (SOCE) signaling. It was further demonstrated that MBL promoted the ubiquitination of Orai1, an essential component of SOCE, via pyruvate dehydrogenase kinase 1 (PDK1)-serum glucocorticoid-regulated kinase 1 signaling. PDK1 inhibition abolished the MBL-mediated regulation of SOCE activity and the EMT process. Notably, biochemical analysis showed that MBL interacted with PDK1 and contributed to PDK1 ubiquitination. In summary, the present findings suggested that MBL limited the EMT phenotype in human alveolar epithelial cells through regulation of SOCE, and MBL could be recognized as a potential therapeutic target for IPF.
DOI: 10.6084/m9.figshare.8848511.v1
title: Banana leaf in the diet of laying hens in cage free system

description: ABSTRACT. The aim of this research was evaluate the productive performance, egg quality and the endoparasites presence on Carijó laying hens in Cage Free system, fed with two experimental diets, one of them supplemented with fresh banana leaf in natura. It were used 196 laying hens with twenty-four weeks of age, distributed in a completely randomized design with 14 boxes, two experimental diets and seven replicates, by treatment with fourteen birds per experimental unit. The variables analyzed were: feed intake; egg production; egg mass: feed conversion per egg mass; feed conversion per dozen eggs; egg weight; albumen weight; yolk weight; shell weight; yolk percentage; albumen percentage shell percentage; yolk pigmentation and the presence of endoparasites. No influence (p &gt; 0.05) were found, for performance and egg production, in Carijó laying hens fed supplemented with banana leaf in natura. For the treatment with inclusion of banana leaf, yolk pigmentation were superior (p &lt; 0.05) when compared to control diet, producing eggs with more yolks orange color. In respect to endoparasite analysis, there was significant effect (p &lt; 0.05) in the percentage of infections present in excretas. Among the treatments evaluated, the one with banana leaf supplementation reduced in 26.54% the endoparasites presence. The inclusion of banana leaf in the diet of Carijo laying hens does not affect performance and eggs quality and decreases the amount of endoparasites of the genus Strogyloides spp.
DOI: 10.6084/m9.figshare.8848511
title: Banana leaf in the diet of laying hens in cage free system

description: ABSTRACT. The aim of this research was evaluate the productive performance, egg quality and the endoparasites presence on Carijó laying hens in Cage Free system, fed with two experimental diets, one of them supplemented with fresh banana leaf in natura. It were used 196 laying hens with twenty-four weeks of age, distributed in a completely randomized design with 14 boxes, two experimental diets and seven replicates, by treatment with fourteen birds per experimental unit. The variables analyzed were: feed intake; egg production; egg mass: feed conversion per egg mass; feed conversion per dozen eggs; egg weight; albumen weight; yolk weight; shell weight; yolk percentage; albumen percentage shell percentage; yolk pigmentation and the presence of endoparasites. No influence (p &gt; 0.05) were found, for performance and egg production, in Carijó laying hens fed supplemented with banana leaf in natura. For the treatment with inclusion of banana leaf, yolk pigmentation were superior (p &lt; 0.05) when compared to control diet, producing eggs with more yolks orange color. In respect to endoparasite analysis, there was significant effect (p &lt; 0.05) in the percentage of infections present in excretas. Among the treatments evaluated, the one with banana leaf supplementation reduced in 26.54% the endoparasites presence. The inclusion of banana leaf in the diet of Carijo laying hens does not affect performance and eggs quality and decreases the amount of endoparasites of the genus Strogyloides spp.
DOI: 10.6084/m9.figshare.20013266
title: Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

description: ABSTRACT. The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products.
DOI: 10.6084/m9.figshare.20013266.v1
title: Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

description: ABSTRACT. The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products.
DOI: 10.6084/m9.figshare.7562651
title: Development and evaluation of prediction equations for methane emission from Nellore cattle

description: ABSTRACT. Dry matter intake (DMI), nutrient intake and enteric CH4 emission were evaluated in 48 Nellore cattle (392 ± 27 days of age). Equations were generated from intake data and evaluated using root mean square prediction error (RMSPE), and validated by cross-validation. Equations that included DMI and hemicellulose intake (HEMI) [ C H 4 M J d - 1 = 4.08 ± 1.65 + 11.6 ± 2.34 D M I k g d - 1 - 33.4 ± 7.21 H E M I ( k g d - 1 ) ]; DMI and total carbohydrate intake (TCHI) [ C H 4 M J d - 1 = 5.26 ± 1.69 - 6.3 ± 1.47 D M I k g d - 1 + 8.8 ± 1.81 T C I k g d - 1 ]; metabolizable energy intake (MEI) and cellulose intake (CELI) [ C H 4 M J d - 1 = 5.16 ± 1.72 - 0.13 ± 0.048 M E I M J d - 1 + 7.37 ± 1.53 C E L I k g d - 1 ], and non-fiber carbohydrate intake (NFCI) [ C H 4 M J d - 1 = 3.14 ± 1.48 + 3.65 ± 1.05 N F C I k g d - 1 ] resulted in the lowest RMSPE (14.3, 14.1, 14.3 and 14.7%, respectively). When literature equations were evaluated using our database, the most accurate predictions were obtained with equations that included DMI and lignin intake (RMSPE = 15.27%) and MEI, acid detergent fiber intake and lignin intake (RMSPE = 15.7%). The mean error of predicting enteric CH4 emission with the equations developed in this study based on DMI and nutrient intake is 17% and the most accurate predictions are obtained with equations including DMI, carbohydrate intake and MEI.
DOI: 10.6084/m9.figshare.20013269.v1
title: Evaluating rumen fermentation kinetics and nutritive value of sunflower seed meal supplemented with acetonic extract of pomegranate peel using in vitro gas production technique

description: ABSTRACT. This study was conducted to investigate effects of supplementing different levels (0.0, 0.5 and 1.0% of buffered rumen fluid) of acetonic extract of pomegranate peel on rumen fermentation kinetics of sunflower seed meal (SFM), using in vitro gas production technique. The samples were incubated in syringes containing rumen liquor obtained from three cannulated Iranian Ghezel rams for 2, 4, 6, 8, 12, 24 and 36h. Results indicated that, addition of acetonic extract of pomegranate peel resulted in increase in gas production volume in all of incubation times (p &lt; 0.0001). Amount of gas production, also increased by increasing dose of the extract. Amounts of a (the gas production from the immediately soluble fraction), b (the gas production from the insoluble fraction) and a + b (the potential gas production) for pomegranate peel extract supplemented sunflower seed meal were higher (p &lt; 0.05) than that of control meal. Adding pomegranate peel extract resulted in increase estimated short chain fatty acids (SCFA) production as well as digestible organic matter (DOM), metabolizable energy (ME) and net energy for lactation (NEL) content of SFM. Production of SCFA as well as DOM, ME and NEL content of SFM increased (p &lt; 0.05) by enhancing the level of the extract supplementation. In conclusion, it can be suggest that, supplementing acetonic extract of pomegranate peel may be lead to higher ruminal fermentation and better nutritive value of SFM in ruminants.
DOI: 10.6084/m9.figshare.20013269
title: Evaluating rumen fermentation kinetics and nutritive value of sunflower seed meal supplemented with acetonic extract of pomegranate peel using in vitro gas production technique

description: ABSTRACT. This study was conducted to investigate effects of supplementing different levels (0.0, 0.5 and 1.0% of buffered rumen fluid) of acetonic extract of pomegranate peel on rumen fermentation kinetics of sunflower seed meal (SFM), using in vitro gas production technique. The samples were incubated in syringes containing rumen liquor obtained from three cannulated Iranian Ghezel rams for 2, 4, 6, 8, 12, 24 and 36h. Results indicated that, addition of acetonic extract of pomegranate peel resulted in increase in gas production volume in all of incubation times (p &lt; 0.0001). Amount of gas production, also increased by increasing dose of the extract. Amounts of a (the gas production from the immediately soluble fraction), b (the gas production from the insoluble fraction) and a + b (the potential gas production) for pomegranate peel extract supplemented sunflower seed meal were higher (p &lt; 0.05) than that of control meal. Adding pomegranate peel extract resulted in increase estimated short chain fatty acids (SCFA) production as well as digestible organic matter (DOM), metabolizable energy (ME) and net energy for lactation (NEL) content of SFM. Production of SCFA as well as DOM, ME and NEL content of SFM increased (p &lt; 0.05) by enhancing the level of the extract supplementation. In conclusion, it can be suggest that, supplementing acetonic extract of pomegranate peel may be lead to higher ruminal fermentation and better nutritive value of SFM in ruminants.
DOI: 10.6084/m9.figshare.8091560
title: Effects of different sources of selenium supplementation on antioxidant indices, biochemical parameters, thyroid hormones and Se status in transition cows

description: ABSTRACT. The aim of this study was to determine the effects of supplementing close-up and fresh dairy cows’ diets with sodium selenite or organic sources of selenium (Se) on the serum biochemical parameters and antioxidant indicators and Se status. Twenty-four multiparous Holstein dairy cows were balanced by body condition score (BCS), previous lactation milk yield and expected calving date and randomly assigned to 1 of 4 experimental treatments. Treatments were: control (basal diet without Se supplementation), sodium selenite supplementation (0.50 mg of Se kg-1 DM; Se-S), selenium yeast supplementation (0.50 mg of Se kg-1 DM; Se-Y) and selenomethionine supplementation (0.50 mg of Se kg-1 DM; Se-M). Serum Se concentrations were higher for cows in Se-Y (72.34 µg dL-1) and Se-Met (72.34 µg dL-1) than control (59.93µg/dl) and Se-S (64.79µg/dl). The Se supplementation and sources did not affect serum metabolites or indices of antioxidant pre and postpartum, except serum total protein and albumin concentrations. Cows in Se-M had greater serum total protein and albumin concentrations than those in control. The results of present study showed that serum Se concentrations increased in Se-Y and Se-M more effectively than Se-S, indicating that selenomethionine could replace Se-S as an effective organic Se source for transition dairy cows.
DOI: 10.6084/m9.figshare.8091560.v1
title: Effects of different sources of selenium supplementation on antioxidant indices, biochemical parameters, thyroid hormones and Se status in transition cows

description: ABSTRACT. The aim of this study was to determine the effects of supplementing close-up and fresh dairy cows’ diets with sodium selenite or organic sources of selenium (Se) on the serum biochemical parameters and antioxidant indicators and Se status. Twenty-four multiparous Holstein dairy cows were balanced by body condition score (BCS), previous lactation milk yield and expected calving date and randomly assigned to 1 of 4 experimental treatments. Treatments were: control (basal diet without Se supplementation), sodium selenite supplementation (0.50 mg of Se kg-1 DM; Se-S), selenium yeast supplementation (0.50 mg of Se kg-1 DM; Se-Y) and selenomethionine supplementation (0.50 mg of Se kg-1 DM; Se-M). Serum Se concentrations were higher for cows in Se-Y (72.34 µg dL-1) and Se-Met (72.34 µg dL-1) than control (59.93µg/dl) and Se-S (64.79µg/dl). The Se supplementation and sources did not affect serum metabolites or indices of antioxidant pre and postpartum, except serum total protein and albumin concentrations. Cows in Se-M had greater serum total protein and albumin concentrations than those in control. The results of present study showed that serum Se concentrations increased in Se-Y and Se-M more effectively than Se-S, indicating that selenomethionine could replace Se-S as an effective organic Se source for transition dairy cows.
DOI: 10.6084/m9.figshare.8091545.v1
title: Performance, ingestive behavior and carcass traits of lambs fed rations containing soybean cake

description: ABSTRACT. The aim of this study was to evaluate the performance, ingestive behavior and carcass traits of crossbred Dorper x Santa Inês lambs fed rations containing soybean cake. Twenty-four lambs (4 months of age and 24.3 ± 3.1 kg BW) were used in a completely randomized design with four treatments and six replicates. Treatments were the inclusion of 50, 100 or 150 g kg-1 of soybean cake in the ration, on a DM basis, and a control diet without this ingredient. Rations were offered ad libitum for 75 days and the lambs were slaughtered at 35 to 40 kg BW. Dry matter intake reduced linearly and the average daily gain had a quadratic response to the soybean cake levels, also decreasing with the addition of this ingredient. The time spent feeding increased and the idle time decreased linearly with the soybean cake levels, whereas the time spent in rumination was not affected. Hot and cold carcass weights decreased linearly with the addition of this ingredient. The addition of 50 to 150 g kg DM-1 of soybean cake to the ration for lambs is not recommended due to reduced animal performance, which may compromise the profitability of sheep farmers.