DOI: 10.6084/m9.figshare.7306373
title: Experimental results of a Lateral Flow Immunochromatographic Strip Test method for rapid detection of Oseltamivir Phosphate in egg and chicken meat

description: This dataset contains experimental results data of a Lateral Flow Immunochromatographic Strip Test (LFIST) method for rapid detection of Oseltamivir Phosphate (OP) in egg and chicken meat.<br>The related study investigates the efficacy of LFIST as an alternative method to High Performance Liquid Chromatography (HPLC) and Liquid Chromatography Mass Spetography (LC-MS), in detection of OP residues in poultry products. OP is an ethyl ester prodrug used to treat and prevent infections from influenza A and B viruses. OP residues can build up in humans from the consumption of affected meat, and cause gastrointestinal disturbances, abnormal neuropsychiatric symptoms and sudden death. In China its use as a veterinary drug has been banned in livestock and poultry, but remains in use illegally, particularly in chicken farming. <br>LFIST can be used to detect OP residues, utilising the specific interaction between antibody and antigen in a sandwich format (or competitive format). The method presents advantages over HPLC and LC-MS, which are dependent on expensive analytical instruments, complex pre-treatment steps, skilled professionals and are not suitable for high-throughput assay or real-time on-site detection.<br>These data files contain the experimental results of LFIST using colloidal gold-labelled monoclonal antibodies (mAbs) as the probe. LFIST was evaluated in term of sensitivity, specificity, and accuracy by testing OP-spiked egg samples and chicken samples. All data are in tabular .<b>xslx</b> spreadsheedt format, accessible via MS Excel and open office software.<br><b>Table1 Titer of polyclonal antiserum against OP.xlsx - </b>contains the determination of titres of mice sera against OP for mice 1 to 5, as well as the blank and negative control samples.<b></b><b><br></b><b>Table 2 Sensitivity of polyclonal antiserum against OP.xlsx </b>- contains the sensitivity values <b></b> of mice sera against OP, for mice 1 to 5 at OP standard concentrations of 0, 1, 2, 4, 8 and 16 ng/mL.<b><br></b><b>Table 3 The indirect ELISA titer of OP mAb.xlsx - </b>contains values <b> </b>for OD450 nm, titre of supernatant and titre of ascites for each mAb hybridoma line reported in the related manuscript: 1D7, 2B3, 2E6 and 4G5<b><br></b><b>Table 4 sensitivity of mAb against OP.xlsx<br></b>contains values of inhibitive titres of mAb against OP for each mAb hybridoma line reported in the related manuscript: 1D7, 2B3, 2E6 and 4G5 at OP standard concentrations of 0, 1, 2, 4, 8 and 16 ng/mL.<b><br></b><b>Table 5 Recovery and intra-assay and inter-assay precision of the test strips for OP spiked.xlsx<br></b>- contains results of accuracy test for LFIST: chicken and egg samples containing 5, 10, and 20 μg/kg of OP were tested using the same batch of strips (n=6). For inter-assay precision, three batches of strips were used in LFIST. Both inter-assay and intra-assay results are reported.<b><br></b><b>Table 6 Comparison of the strip test with HPLC for three levels of OP residues in sample.xlsx</b><br>- contains comparative results of the LFIST and HPLC methods for detection of OP in both chicken and egg samples at OP concentrations of 3.5, 9.7, and 27.9 μg/kg. Results from two methods were compared to the given concentrations by one-sample T test respectively and analyzed by independent-sample T test.<b><br></b>