DOI: 10.5061/dryad.b41j15h
title: Data from: Human retinal pigment epithelium: in vivo cell morphometry, multispectral autofluorescence, and relationship to cone mosaic

description: Purpose: To characterize in vivo morphometry and multispectral
 autofluorescence of the retinal pigment epithelial (RPE) cell mosaic and
 its relationship to cone cell topography across the macula. Methods: RPE
 cell morphometrics were computed in regularly spaced regions of interest
 (ROIs) from contiguous short-wavelength autofluorescence (SWAF) and
 photoreceptor reflectance images collected across the macula in one eye of
 10 normal participants (23–65 years) by using adaptive optics scanning
 light ophthalmoscopy (AOSLO). Infrared autofluorescence (IRAF) images of
 the RPE were collected with AOSLO in seven normal participants (22–65
 years), with participant overlap, and compared to SWAF quantitatively and
 qualitatively. Results: RPE cell statistics could be analyzed in 84% of
 SWAF ROIs. RPE cell density consistently decreased with eccentricity from
 the fovea (participant mean ± SD: 6026 ± 1590 cells/mm2 at fovea; 4552 ±
 1370 cells/mm2 and 3757 ± 1290 cells/mm2 at 3.5 mm temporally and nasally,
 respectively). Mean cone-to-RPE cell ratio decreased rapidly from 16.6 at
 the foveal center to &lt;5 by 1 mm. IRAF revealed cells in six of
 seven participants, in agreement with SWAF RPE cell size and location.
 Differences in cell fluorescent structure, contrast, and visibility
 beneath vasculature were observed between modalities. Conclusions:
 Improvements in AOSLO autofluorescence imaging permit efficient
 visualization of RPE cells with safe light exposures, allowing individual
 characterization of RPE cell morphometry that is variable between
 participants. The normative dataset and analysis of RPE cell IRAF and SWAF
 herein are essential for understanding microscopic characteristics of cell
 fluorescence and may assist in interpreting disease progression in RPE
 cells.
description: NOR011This folder contains
 data for participant NOR011, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR025This folder contains
 data for participant NOR011, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR047This folder contains
 data for participant NOR047, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR053This folder contains
 data for participant NOR053, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR057This folder contains
 data for participant NOR057, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR062This folder contains
 data for participant NOR062, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR063This folder contains
 data for participant NOR063, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR064This folder contains
 data for participant NOR064, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR065This folder contains
 data for participant NOR065, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.NOR073This folder contains
 data for participant NOR073, including the full-size montage (.tiff) of
 the retinal pigment epithelium (RPE) cell mosaic, and individual regions
 of interest (ROIs) used for analysis in the study. High resolution retinal
 images were acquired in the living human eye using an adaptive optics
 scanning light ophthalmoscope.