cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/j8ztt-x0f62
title: Agro pJW58

description: wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter.  Kanamycin-resistant (150 to 300 ug/mL)
description: <strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-8/rawdata/</p>4_pJW58_20001.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-8/rawdata/4_pJW58_20001.mrc"
        > <i class="download icon"></i> Download </a></p>

        4_pJW58_20001_part121_1.rec,  Reconstruction (Pixel Size 1.6 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-8/3dimage_39687/4_pJW58_20001_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s2env-wqz96
title: Alongum

description: Alongum_periplasmiclayers
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-2/rawdata/</p>AlongLys0003.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-2/rawdata/AlongLys0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        AlongLys0003_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-2/3dimage_56719/AlongLys0003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jdk55-x7925
title: HUVECs infx pNLBg1I

description: Raw data files of HUVECs infx pNLBg1I
description: <strong>Tilt Series Date:</strong> 2013-10-18</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen endpoint of virus infected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-18-12/rawdata/</p>13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0012.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-18-12/rawdata/13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0012_part81_1.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-18-12/3dimage_32855/13oct18_C179-2_pNLBglI_Endpointvirus_AT1BT1_0012_part81_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gm0kh-6h498
title: HUVECs 24h PI pNLBglI

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 6500
description: <strong>Tilt Series Date:</strong> 2013-04-30</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 6500x. </p>
<strong>Microscope:</strong> UCLA Titan</p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> FEIbatchtomo</p>
<strong>Collaborators and Roles:</strong> Collected at UCLA with FEI Batchtomo</p>
<strong>Sample Preparation:</strong> HUVECs imaged 24 hours after infection with pNLBglI. The goal is to image maturing HIV at the edge of infected cells.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-15/rawdata/</p>13apr26_tomo_21.mrc,  Tilt Series (Pixel Size 1.35 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-15/rawdata/13apr26_tomo_21.mrc"
        > <i class="download icon"></i> Download </a></p>

        13apr26_tomo_21_full.rec,  Reconstruction (Pixel Size 2.7 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-15/3dimage_26331/13apr26_tomo_21_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v8fs6-skc71
title: HeLa cells

description: Looking for ESCRT around midbodies
description: <strong>Tilt Series Date:</strong> 2014-11-26</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> Cells provided by Doug Mckarty</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA nup153+Thymidine 2mM for 14 h.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-46/rawdata/</p>midbody50010.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-46/rawdata/midbody50010.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody50010_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-11-26-46/3dimage_48425/midbody50010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zc2zm-fpz03
title: MJ007 sacculi

description: sacculi purification of sporulating cells
description: <strong>Tilt Series Date:</strong> 2012-03-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kit Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> See E Tocheva Mol Micro 2013</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-19/rawdata/</p>mj0008.mrc,  Tilt Series (Pixel Size 1.9 nm), 925.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-19/rawdata/mj0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        mj0008_part112_6.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-19/3dimage_17649/mj0008_part112_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ax94v-6t573
title: Sulfolobus solfactaricus+c92

description: Sulfolobus solfactaricus PH1-16 cells, transfected with C92 pyramid protein. 24 H. Post transfection.
description: <strong>Tilt Series Date:</strong> 2014-01-06</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> Ph1-16</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder.</p>
<strong>Sample Preparation:</strong> With Jamie Synder</p>
  rra2014-01-06-7_slicer6720.jpg: Unknown filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-7/rawdata/</p>ssop1c920001.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-7/rawdata/ssop1c920001.mrc"
        > <i class="download icon"></i> Download </a></p>

        ssop1c920001_part111_4.rec,  Reconstruction (Pixel Size 2.52 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-7/3dimage_37472/ssop1c920001_part111_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2rx28-na398
title: Agro C1 induced at 19C

description: Agro C1, no Ti plasmid, induced at 19 in liquid for 24h  AB+AS
description: <strong>Tilt Series Date:</strong> 2014-03-26</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Agro C1, no Ti plasmid, induced at 19 in liquid AB+AS</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-26-14/rawdata/</p>Agro_C10014.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-26-14/rawdata/Agro_C10014.mrc"
        > <i class="download icon"></i> Download </a></p>

        Agro_C10014.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-26-14/3dimage_49446/Agro_C10014.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.qjd5he
title: Occurrence Download

description: A dataset containing 4549 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pterorhinus davidi",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4549 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045856-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/apzye-sng07
title: Agrobacterium tumefaciens

description: C58 grown on slide
description: <strong>Tilt Series Date:</strong> 2013-10-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on a glass slide</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-13/rawdata/</p>c58_slide_2750021.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-13/rawdata/c58_slide_2750021.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_slide_2750021_full.rec,  Reconstruction (Pixel Size 1.56 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-13/3dimage_31957/c58_slide_2750021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jzcxa-zfw91
title: JT5 on xylose

description: JT5
description: <strong>Tilt Series Date:</strong> 2014-07-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacteroidetes</p>
<strong>Strain:</strong> JT5-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Eric Matson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown on xylose as a carbon source</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-07-20-7/rawdata/</p>JT5_xyl0007.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-07-20-7/rawdata/JT5_xyl0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        JT5_xyl0007_full.rec,  Reconstruction (Pixel Size 0.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-07-20-7/3dimage_49407/JT5_xyl0007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5q3t9-cv470
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-13</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-24/rawdata/</p>12mar13_c121_1_Cos74ADN_AT1bt1_0018.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-24/rawdata/12mar13_c121_1_Cos74ADN_AT1bt1_0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar13_c121_1_Cos74ADN_AT1bt1_0018_full.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-24/3dimage_17821/12mar13_c121_1_Cos74ADN_AT1bt1_0018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wymac-e1w72
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2012-11-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-49/rawdata/</p>Bdellovibrio_wt_20121114_0030008.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-49/rawdata/Bdellovibrio_wt_20121114_0030008.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdellovibrio_wt_20121114_0030008_full.rec,  Reconstruction (Pixel Size 1.32 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-49/3dimage_23568/Bdellovibrio_wt_20121114_0030008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x18fz-1pv53
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-05-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-14/3dimage_69225/</p>12may04_C126_3_pnlDN_Cos7_AT1BT2_0015_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
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        12may04_C126_3_pnlDN_Cos7_AT1BT2_0015.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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