cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/7k4vp-59313
title: Legionella_delta-lspGHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS major and minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-18</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture, OD~4.8); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-18-25/rawdata/</p>DG8_201905180012.mrc,  Tilt Series (Pixel Size 0.26 nm), 882.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-18-25/rawdata/DG8_201905180012.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG8_201905180012_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-18-25/3dimage_102926/DG8_201905180012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qs1fm-x2j74
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-01</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-27/rawdata/</p>Test_Pseudomonas_dfliF_27_fix.mrc,  Tilt Series (Pixel Size 0.37 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-27/rawdata/Test_Pseudomonas_dfliF_27_fix.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_27_fix_part61_2.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-01-27/3dimage_97323/Test_Pseudomonas_dfliF_27_fix_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.m5q2cu
title: Occurrence Download

description: A dataset containing 5637 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-95.77791 48.56533,-97.26305 44.32991,-91.21707 39.1264,-85.41888 38.33349,-80.2722 37.77443,-78.43798 37.43051,-77.44444 37.35409,-76.48911 37.04838,-75.73191 36.94901,-74.49813 38.39015,-73.74937 40.29312,-71.67186 41.0095,-69.93662 41.08114,-69.84896 41.54295,-69.51465 43.5329,-67.65206 44.25725,-66.96751 44.64462,-66.95159 44.8277,-67.16651 45.19385,-67.50878 45.24161,-67.50082 45.43264,-67.38142 45.5998,-67.76349 45.80675,-67.78737 46.37986,-67.81125 47.04052,-68.25877 47.33516,-68.89515 47.18923,-69.02293 47.41284,-69.24654 47.4927,-70.05314 46.71805,-70.04515 46.4545,-70.85967 45.24627,-71.90456 44.99908,-74.6896 45.00826,-75.27528 44.85696,-75.76 44.51404,-75.82004 44.43463,-75.91717 44.36627,-76.05524 44.30625,-76.18745 44.28781,-76.15363 44.23554,-76.23818 44.20172,-76.30736 44.19864,-76.32405 44.19095,-76.3548 44.12792,-76.4332 44.0941,-76.78551 43.63373,-77.79831 43.64295,-78.80521 43.62057,-79.07273 43.27945,-79.0487 43.14556,-79.06758 43.12667,-79.07273 43.07174,-78.9972 43.06144,-79.01951 43.03055,-79.02466 42.97905,-78.94055 42.9533,-78.90794 42.93613,-78.90816 42.8869,-80.08166 42.42714,-82.58405 41.64203,-82.77291 41.74446,-83.02258 41.77967,-83.14713 42.01693,-83.13433 42.2282,-83.05751 42.31782,-82.90066 42.34983,-82.67013 42.513,-82.5805 42.54821,-82.51968 42.65385,-82.48127 42.8203,-82.41084 42.99635,-82.22133 44.6402,-83.90064 45.92645,-83.88348 46.02367,-84.08937 46.18381,-84.08937 46.30391,-84.30097 46.49836,-84.6384 46.49836,-84.73293 46.73284,-88.27638 48.32441,-89.03701 48.07277,-89.73462 48.02201,-95.77791 48.56533))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Ambystoma laterale"
 ]
}

 The dataset includes 5637 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048034-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ayx2t-93d28
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-214/rawdata/</p>9082_box2_No3_101.mrc,  Tilt Series (Pixel Size 0.2612 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-214/rawdata/9082_box2_No3_101.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_box2_No3_101.rec,  Reconstruction (Pixel Size 1.0448 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-214/3dimage_110518/9082_box2_No3_101.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kyfcp-68763
title: Pseudomonas aeruinosa

description: This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
description: <strong>Tilt Series Date:</strong> 2018-10-03</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Serial EM</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newmann lab (Shannon Park)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight at 37 C in LB with 100 ug/ml gentamicin</p>
<strong>Sample Preparation:</strong> Samples from the overnight culture were used to prepare the grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-03-6/rawdata/</p>Test_Pseudomonas_dfliF_105.mrc,  Tilt Series (Pixel Size 0.37 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-03-6/rawdata/Test_Pseudomonas_dfliF_105.mrc"
        > <i class="download icon"></i> Download </a></p>

        Test_Pseudomonas_dfliF_105_full.rec,  Reconstruction (Pixel Size 1.48 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-10-03-6/3dimage_97425/Test_Pseudomonas_dfliF_105_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ebntfm
title: Occurrence Download

description: A dataset containing 565518 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Plant Resources Center"
 ]
}

 The dataset includes 565518 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048022-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/eewcn-m1028
title: Legionella_delta-lspHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-103/rawdata/</p>DG7_20190516_tomo0103.mrc,  Tilt Series (Pixel Size 0.26 nm), 882.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-103/rawdata/DG7_20190516_tomo0103.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG7_20190516_tomo0103_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-103/3dimage_102821/DG7_20190516_tomo0103_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d6s0s-h3816
title: bio12-2_lam5_ts4

description: .  [added 2019-10-11 16:53:00] IT 0.8s
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: °, constant angular increment, dosage: 88.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-16/rawdata/</p>bio12-2_lam5_ts4.st,  Tilt Series (Pixel Size 0.48 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-16/rawdata/bio12-2_lam5_ts4.st"
        > <i class="download icon"></i> Download </a></p>

        bio12-2_lam5_ts4.rec,  Reconstruction (Pixel Size 1.8 nm), 278.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-16/3dimage_106818/bio12-2_lam5_ts4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jksq9-krf08
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-131/3dimage_123942/</p>JV9298_PD-1-3_500_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-131/3dimage_123942/JV9298_PD-1-3_500_SIRT_1k.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_PD-1-3_500.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-131/rawdata/JV9298_PD-1-3_500.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gyg83-ss244
title: U937 infection

description: Lp02 pCyaA::RalF-Ub V76 .  U937
description: <strong>Tilt Series Date:</strong> 2020-07-31</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, 1-2 washes, fibronectin/vitronectin/no matrix. Legionella: 2.5 O.D, 3h after 200mM  IPTG induction, at O.D. 3</p>
<strong>Sample Preparation:</strong> 200mesh Gold quantifoil,  10nm gold, 37deg C, 80-90% humidity</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-8/rawdata/</p>tomography12_168.mrc,  Tilt Series (Pixel Size 0.265 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-8/rawdata/tomography12_168.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography12_168_full.rec,  Reconstruction (Pixel Size 1.06 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-8/3dimage_113168/tomography12_168_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.beaktp
title: Occurrence Download

description: A dataset containing 787 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "PublishingCountry is Colombia",
 {
 "and" : [
 "Country is Colombia",
 {
 "not" : {
 "Issue" : [
 "is Country coordinate mismatch"
 ]
 }
 }
 ]
 }
 ]
 },
 {
 "and" : [
 "TaxonKey is one of (Alouatta seniculus [GBIF Backbone Taxonomy])",
 "StateProvince is one of (Meta, Casanare, Vichada, Arauca)"
 ]
 }
 ]
}

 The dataset includes 787 records from 83 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048037-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/axjdq-fm143
title: MEF cells with FCCP

description: To study mitochondrial division. Grid - WY43_grid3.
description: <strong>Tilt Series Date:</strong> 2018-07-29</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -2.5 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD and tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze grids. Shrawan FIB-milled cells and performed ECT. Cells courtesy of Chan lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were treated for 3 min with 10 µM FCCP before plunge freezing and FIB-milling.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-29-13/rawdata/</p>TS13.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-29-13/rawdata/TS13.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS13_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 962.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-29-13/3dimage_102084/TS13_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5213b-yet52
title: RalF-Ub V76

description: Lp02 pCyaA::RalF-Ub V76 free from U937
description: <strong>Tilt Series Date:</strong> 2020-07-31</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, 1-2 washes, fibronectin/vitronectin/no matrix. Legionella: 2.5 O.D, 3h after 200mM  IPTG induction, at O.D. 3</p>
<strong>Sample Preparation:</strong> 200mesh Gold quantifoil,  10nm gold, 37deg C, 80-90% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-41/rawdata/</p>RalFUbV76_175.mrc,  Tilt Series (Pixel Size 0.265 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-41/rawdata/RalFUbV76_175.mrc"
        > <i class="download icon"></i> Download </a></p>

        RalFUbV76_175_full.rec,  Reconstruction (Pixel Size 1.06 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-41/3dimage_113246/RalFUbV76_175_full.rec"
        > <i class="download icon"></i> Download </a></p>