DOI: 10.22002/xpwpq-5ya51
title: NR1769pKM101conjFM433

description: skinny ecoli conjugation with FM433 ecoli
description: <strong>Tilt Series Date:</strong> 2014-10-25</p>
 
<strong>Data Taken By:</strong> Elitza Tocheva</p>
 
<strong>Species / Specimen:</strong> Escherichia coli</p>
 
<strong>Strain:</strong> NR1769 pKM101</p>
 
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å², defocus: -10.0 μm, magnification: 18000x. </p>
 
<strong>Microscope:</strong> Caltech Polara</p>
 
<strong>Acquisition Software:</strong> UCSFTomo</p>
 
<strong>Upload Method:</strong> reupload_downloaded</p>
 
<strong>Processing Software Used:</strong> IMOD</p>
 
<strong>Collaborators and Roles:</strong> Christian Baron</p>
 
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown on a gold grid in a drop of 1:1 mix of bacteria for 30min</p>
 
<strong>Sample Preparation:</strong> Vitrobot</p>
 
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/rawdata/</p>
 
NRpKMplusFM433_conj1_18K0011.mrc, Tilt Series (Pixel Size 0.63 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/rawdata/NRpKMplusFM433_conj1_18K0011.mrc" > <i class="download icon"></i> Download </a>
</p>
 
 NRpKMplusFM433_conj1_18K0011_full.rec, Reconstruction (Pixel Size 0.0 nm), 980.0 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/3dimage_49678/NRpKMplusFM433_conj1_18K0011_full.rec" > <i class="download icon"></i> Download </a>
</p>
 
 slicer000.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12393/slicer000.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer001.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12394/slicer001.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer002.tif, 2.6 MB
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</p>
 
 slicer003.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12396/slicer003.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer004.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12397/slicer004.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer005.tif, 2.6 MB
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</p>
 
 slicer006.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12399/slicer006.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer007.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12400/slicer007.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer008.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12401/slicer008.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer009.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12402/slicer009.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer010.tif, 2.6 MB
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</p>
 
 slicer011.tif, 2.6 MB
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</p>
 
 slicer012.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12405/slicer012.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer013.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12406/slicer013.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer014.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12407/slicer014.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer015.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12408/slicer015.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer016.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12409/slicer016.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer017.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12410/slicer017.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer018.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12411/slicer018.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer019.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12412/slicer019.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer020.tif, 2.6 MB
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</p>
 
 slicer021.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12414/slicer021.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer022.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-16/file_12415/slicer022.tif" > <i class="download icon"></i> Download </a>
</p>