cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21793283.v1
title: Fig1B_qPCR_G6PD_WT-A549_R1_ExtFig3B_HA_Xrn1ko_R2_LG292.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793304
title: Fig1B_qPCR_G6PD_WT-A549_R2_ExtFig3B_HA_Xrn1ko_R3_LG293.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793307.v1
title: Fig1B_qPCR_G6PD_WT-A549_R3_LG400.xlsx

description: A549 or A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793475.v1
title: Fig2F_STOML2_ExtFig4C_BCAP31_RACE_R1_LG295.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793304.v1
title: Fig1B_qPCR_G6PD_WT-A549_R2_ExtFig3B_HA_Xrn1ko_R3_LG293.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793319
title: Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R1_LG258.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793319.v1
title: Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R1_LG258.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21921768
title: References

description: This data contain the references of the libriary research
DOI: 10.6084/m9.figshare.21921768.v1
title: References

description: This data contain the references of the libriary research
DOI: 10.6084/m9.figshare.21800432
title: Fig5C_STOML2_intron_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21800432.v1
title: Fig5C_STOML2_intron_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21800438
title: Fig5C_STOML2_intron_R1-R2-R3_LG290-292-293.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21800438.v1
title: Fig5C_STOML2_intron_R1-R2-R3_LG290-292-293.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21800444
title: Fig5C_STOML2_intron_R1-R2-R3_LG290-292-293_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21800444.v1
title: Fig5C_STOML2_intron_R1-R2-R3_LG290-292-293_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21761042
title: Antimicrobial Resistance, Extended-Spectrum β-Lactamase Production and Virulence Genes in Salmonella enterica and Escherichia coli Isolates from Estuarine Environment

description: The raw data of phenotype and genotype of antimicrobial resistant, ESBL production and virulence genes of <em>Salmonella </em>and <em>E. coli</em> isolated from estuarine environment.
DOI: 10.6084/m9.figshare.21797219
title: Fig2G_RACE_STOML2_R2_LG364_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797219.v1
title: Fig2G_RACE_STOML2_R2_LG364_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797225
title: Fig2G_RACE_STOML2_R3_LG366.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797225.v1
title: Fig2G_RACE_STOML2_R3_LG366.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797234
title: Fig2G_RACE_YKT6_R3_LG366_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797234.v1
title: Fig2G_RACE_YKT6_R3_LG366_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797252
title: Fig2G_RACE_YKT6_R4_LG367_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797561
title: Fig2G_RACE_YKT6_R4_LG367.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797561.v1
title: Fig2G_RACE_YKT6_R4_LG367.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797252.v1
title: Fig2G_RACE_YKT6_R4_LG367_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797270
title: Fig2G_RACE_STOML2_R2_LG364.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797276
title: Fig2G_RACE_STOML2_R4_LG367.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797270.v1
title: Fig2G_RACE_STOML2_R2_LG364.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797276.v1
title: Fig2G_RACE_STOML2_R4_LG367.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797294
title: Fig2G_RACE_YKT6_R1_LG361.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797294.v1
title: Fig2G_RACE_YKT6_R1_LG361.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797309
title: Fig2G_RACE_YKT6_R4_LG367.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797309.v1
title: Fig2G_RACE_YKT6_R4_LG367.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797321
title: Fig2G_RACE_YKT6_R3_LG366.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797321.v1
title: Fig2G_RACE_YKT6_R3_LG366.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797336
title: Fig2G_RACE_YKT6_R2_LG364.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797336.v1
title: Fig2G_RACE_YKT6_R2_LG364.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797339
title: Fig2G_RACE_YKT6_R1_LG361_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797339.v1
title: Fig2G_RACE_YKT6_R1_LG361_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797330
title: Fig2G_RACE_YKT6_R4_LG367.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21797330.v1
title: Fig2G_RACE_YKT6_R4_LG367.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.