cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11966400
title: Expression of miR-148b-3p is correlated with overexpression of biomarkers in prostate cancer

description: Abstract Prostate cancer (PCa) is one of the leading causes of death among men. Genes such as PCA3, PSA, and Fra-1 are suggested to serve as potential tools for the detection of PCa, as they are deregulated during this pathology. A similar event occurs with small non-coding RNAs, called miRNAs, specifically miR-195-5p, miR-133a-3p, and miR-148b-3p, which were analyzed in a Chinese population and suggested to be possible candidates for PCa diagnosis. We evaluated the expression levels of three miRNAs and three genes in tissue samples of PCa and benign prostate disease, such as benign prostatic hyperplasia, or prostatitis, in order to determine their potential as candidates for PCa detection. Our results showed a statistically significant overexpression of 279-fold increase in PSA levels and a 1,012-fold increase in PCA3 levels in PCa patients compared to benign prostate disease patients (p = 0.001 and p = 0.002, respectively). We observed a positive correlation between the expression of miR-148b-3p and the expression of PSA and PCA3 genes, two established biomarkers in PCa. The expression of miR-148b-3p was not related to clinical characteristics, such as age and weight, as observed for the other miRNAs analyzed, suggesting its potential as a biomarker for detection of this pathology.
DOI: 10.6084/m9.figshare.11966403
title: Light Activation and Thermocycling Methods on the Shear Bond Strength of Brackets Bonded to Porcelain Surfaces

description: Abstract The present study was evaluated the effect of different light activation and thermocycling methods on the shear bond strength (SBS) and on the adhesive remnant index (ARI) of metal brackets bonded to feldspathic ceramic. Hundred metal brackets were bonded to 20 porcelain cylinders, divided into four groups (n=25) based on light activation and thermocycling processes. The cylinders were etched with 10% hydrofluoric acid for 60 s and coated with two layers of silane. The brackets were bonded with Transbond XT composite resin. Light activation in Groups 1 and 3 was performed during 3 s using the VALO Ortho Cordless appliance with irradiance 3,200 mW/cm2 and in Groups 2 and 4 for 40 s using Optilight Max appliance with irradiance 1,200 mW/cm². The samples were stored in deionized water at 37°C for 24 h and the samples from Groups 1 and 2 were submitted to the SBS test at a rate of 1 mm/min, whereas the samples from Groups 3 and 4 were submitted to 7,000 thermal cycles (5°/55°C) before to the SBS test. The data were assessed by two-way analysis of variance and by Tukey’s test (a=0.05). No significant difference was observed between SBS means in the different light activation devices used. The samples subjected to thermocycling revealed lower SBS values (p≤0.05). There was predominance of score 0 for ARI in all groups. Therefore, the different light activation methods did not interfere in SBS, but thermocycling reduced SBS.
DOI: 10.6084/m9.figshare.11966403.v1
title: Light Activation and Thermocycling Methods on the Shear Bond Strength of Brackets Bonded to Porcelain Surfaces

description: Abstract The present study was evaluated the effect of different light activation and thermocycling methods on the shear bond strength (SBS) and on the adhesive remnant index (ARI) of metal brackets bonded to feldspathic ceramic. Hundred metal brackets were bonded to 20 porcelain cylinders, divided into four groups (n=25) based on light activation and thermocycling processes. The cylinders were etched with 10% hydrofluoric acid for 60 s and coated with two layers of silane. The brackets were bonded with Transbond XT composite resin. Light activation in Groups 1 and 3 was performed during 3 s using the VALO Ortho Cordless appliance with irradiance 3,200 mW/cm2 and in Groups 2 and 4 for 40 s using Optilight Max appliance with irradiance 1,200 mW/cm². The samples were stored in deionized water at 37°C for 24 h and the samples from Groups 1 and 2 were submitted to the SBS test at a rate of 1 mm/min, whereas the samples from Groups 3 and 4 were submitted to 7,000 thermal cycles (5°/55°C) before to the SBS test. The data were assessed by two-way analysis of variance and by Tukey’s test (a=0.05). No significant difference was observed between SBS means in the different light activation devices used. The samples subjected to thermocycling revealed lower SBS values (p≤0.05). There was predominance of score 0 for ARI in all groups. Therefore, the different light activation methods did not interfere in SBS, but thermocycling reduced SBS.
DOI: 10.6084/m9.figshare.11966406.v2
title: Supplementary data .docx

description: depression
DOI: 10.6084/m9.figshare.11966400.v1
title: Expression of miR-148b-3p is correlated with overexpression of biomarkers in prostate cancer

description: Abstract Prostate cancer (PCa) is one of the leading causes of death among men. Genes such as PCA3, PSA, and Fra-1 are suggested to serve as potential tools for the detection of PCa, as they are deregulated during this pathology. A similar event occurs with small non-coding RNAs, called miRNAs, specifically miR-195-5p, miR-133a-3p, and miR-148b-3p, which were analyzed in a Chinese population and suggested to be possible candidates for PCa diagnosis. We evaluated the expression levels of three miRNAs and three genes in tissue samples of PCa and benign prostate disease, such as benign prostatic hyperplasia, or prostatitis, in order to determine their potential as candidates for PCa detection. Our results showed a statistically significant overexpression of 279-fold increase in PSA levels and a 1,012-fold increase in PCA3 levels in PCa patients compared to benign prostate disease patients (p = 0.001 and p = 0.002, respectively). We observed a positive correlation between the expression of miR-148b-3p and the expression of PSA and PCA3 genes, two established biomarkers in PCa. The expression of miR-148b-3p was not related to clinical characteristics, such as age and weight, as observed for the other miRNAs analyzed, suggesting its potential as a biomarker for detection of this pathology.
DOI: 10.6084/m9.figshare.11966406.v1
title: Supplementary data .docx

description: depression
DOI: 10.6084/m9.figshare.11966394
title: Genetic variants in the fat mass and obesity-associated (FTO) gene confer risk for extreme obesity and modulate adiposity in a Brazilian population

description: Abstract Obesity is a major public health problem worldwide. It has a complex etiology, influenced by environmental and genetic factors. FTO has been recognized as an important genetic factor for obesity development. This study evaluated the contribution of FTO polymorphisms (rs9939609 and rs17817449) for extreme obesity in terms of the period of obesity onset, anthropometric, and biochemical parameters. The haplotype and the combined effects of FTO risk alleles on obesity susceptibility were evaluated. We investigated 169 normal-weight subjects (body mass index, BMI: 22.8 [21.0; 24.0] kg/m2) and 123 extremely obese individuals (BMI: 47.6 [44.1; 53.1] kg/m2). Genotyping was performed by real time PCR. Our results showed a strong association between FTO variants and extreme obesity. Carriers of the AT haplotype had an increased risk for extreme obesity. Gene scores suggested that the risk of developing extreme obesity was increased 1.37-fold per risk allele added. Both polymorphisms also influenced BMI and body weight. Additionally, rs17817449 influenced triglyceride levels. No effect of FTO variants on the period of obesity onset was found. In conclusion, the FTO polymorphisms showed a strong association with development of extreme phenotype of obesity and adiposity modulation in a Brazilian population.
DOI: 10.6084/m9.figshare.11966394.v1
title: Genetic variants in the fat mass and obesity-associated (FTO) gene confer risk for extreme obesity and modulate adiposity in a Brazilian population

description: Abstract Obesity is a major public health problem worldwide. It has a complex etiology, influenced by environmental and genetic factors. FTO has been recognized as an important genetic factor for obesity development. This study evaluated the contribution of FTO polymorphisms (rs9939609 and rs17817449) for extreme obesity in terms of the period of obesity onset, anthropometric, and biochemical parameters. The haplotype and the combined effects of FTO risk alleles on obesity susceptibility were evaluated. We investigated 169 normal-weight subjects (body mass index, BMI: 22.8 [21.0; 24.0] kg/m2) and 123 extremely obese individuals (BMI: 47.6 [44.1; 53.1] kg/m2). Genotyping was performed by real time PCR. Our results showed a strong association between FTO variants and extreme obesity. Carriers of the AT haplotype had an increased risk for extreme obesity. Gene scores suggested that the risk of developing extreme obesity was increased 1.37-fold per risk allele added. Both polymorphisms also influenced BMI and body weight. Additionally, rs17817449 influenced triglyceride levels. No effect of FTO variants on the period of obesity onset was found. In conclusion, the FTO polymorphisms showed a strong association with development of extreme phenotype of obesity and adiposity modulation in a Brazilian population.
DOI: 10.6084/m9.figshare.11966385
title: The politics of Brazil’s access to information policies: history and coalitions

description: Abstract How did the various legislative initiatives on access to information in Brazil culminate in the 12.527/11 federal law? Using the Advocacy Coalition Framework, this article analyzes the agenda setting and strategies of different coalitions, analyzing a broad array of news, interviews, laws, and official documents between 2001 and 2012. Two coalitions were identified: one in favor of changes in legislation, comprised of sectors of the Executive, legislative, the media and civil society; and another in favor of the maintenance of restrictive rules of access to information, composed of Armed Forces, Itamaraty (Brazil’s diplomatic corps) and the Senate’s Legislative Commission on Defense and Foreign Affairs. The article also identifies an overlap of two public policy subsystems: the transparency subsystem and the reconciliation one. The latter monopolized the public debate on the subject, contributing to the slowness in approving a transparency law. Itamaraty also emerges as the author of a strong lobby against changes in the rules for document classification, for reasons yet to be studied.
DOI: 10.6084/m9.figshare.11966385.v1
title: The politics of Brazil’s access to information policies: history and coalitions

description: Abstract How did the various legislative initiatives on access to information in Brazil culminate in the 12.527/11 federal law? Using the Advocacy Coalition Framework, this article analyzes the agenda setting and strategies of different coalitions, analyzing a broad array of news, interviews, laws, and official documents between 2001 and 2012. Two coalitions were identified: one in favor of changes in legislation, comprised of sectors of the Executive, legislative, the media and civil society; and another in favor of the maintenance of restrictive rules of access to information, composed of Armed Forces, Itamaraty (Brazil’s diplomatic corps) and the Senate’s Legislative Commission on Defense and Foreign Affairs. The article also identifies an overlap of two public policy subsystems: the transparency subsystem and the reconciliation one. The latter monopolized the public debate on the subject, contributing to the slowness in approving a transparency law. Itamaraty also emerges as the author of a strong lobby against changes in the rules for document classification, for reasons yet to be studied.
DOI: 10.6084/m9.figshare.11966379
title: Color Stability of Ceramic Veneers Luted With Resin Cements and Pre-Heated Composites: 12 Months Follow-Up

description: Abstract The objective was to evaluate the color stability of ceramic veneers luted with resin cements and pre-heated composite resins (60oC) for 12 months, and determine the degree of conversion (DC) of the luting agents. Two resin cements (AllCem Veneer, light-cured (LRC) and AllCem, dual-cured (DRC)] and three composite resins [Z100 (MNCR-minifilled), Herculite Classic (MHCR-micro-hybrid) and Durafill (MCCR-microfilled)] were used for cementing 0.8-mm-thick lithium-silicate glass-ceramic laminates (Suprinity, shade B2-HT, Vita) on bovine enamel (n=10). The specimens were stored at 37oC in distilled water. CIELab parameters were determined at 24h after luting (baseline), 7, 30, 90, 180 days and 12 months. Three specimens were prepared for DC evaluation, performed by micro-Raman spectroscopy. Data were analyzed by ANOVA and Tukey’s test (a=5%). For ΔEab and ΔE00, there were significant differences for luting material (p&lt;0.001), time (p&lt;0.001), and double interaction (p&lt;0.001). The groups cemented with MHCR (1 year), MCCR (90 days and 1 year) and MCCR-PH (1 year) were the ones with ΔE values greater than the acceptability threshold. All other groups maintained their ΔE lower than the acceptability threshold after 1 year in distilled water. Regarding DC, there were no significant differences (p=0.127) among the materials. Non-significant negative correlations were observed between the mean ΔEab and DC (R=-0.65) and ΔE00 and DC (R=-0.64). A significant positive correlation was observed mean ΔEab and ΔE00 (R=0.99). It was concluded that the different luting agents influenced the final color of the restorations. The heating of the composite resins did not affect their DC.
DOI: 10.6084/m9.figshare.11966379.v1
title: Color Stability of Ceramic Veneers Luted With Resin Cements and Pre-Heated Composites: 12 Months Follow-Up

description: Abstract The objective was to evaluate the color stability of ceramic veneers luted with resin cements and pre-heated composite resins (60oC) for 12 months, and determine the degree of conversion (DC) of the luting agents. Two resin cements (AllCem Veneer, light-cured (LRC) and AllCem, dual-cured (DRC)] and three composite resins [Z100 (MNCR-minifilled), Herculite Classic (MHCR-micro-hybrid) and Durafill (MCCR-microfilled)] were used for cementing 0.8-mm-thick lithium-silicate glass-ceramic laminates (Suprinity, shade B2-HT, Vita) on bovine enamel (n=10). The specimens were stored at 37oC in distilled water. CIELab parameters were determined at 24h after luting (baseline), 7, 30, 90, 180 days and 12 months. Three specimens were prepared for DC evaluation, performed by micro-Raman spectroscopy. Data were analyzed by ANOVA and Tukey’s test (a=5%). For ΔEab and ΔE00, there were significant differences for luting material (p&lt;0.001), time (p&lt;0.001), and double interaction (p&lt;0.001). The groups cemented with MHCR (1 year), MCCR (90 days and 1 year) and MCCR-PH (1 year) were the ones with ΔE values greater than the acceptability threshold. All other groups maintained their ΔE lower than the acceptability threshold after 1 year in distilled water. Regarding DC, there were no significant differences (p=0.127) among the materials. Non-significant negative correlations were observed between the mean ΔEab and DC (R=-0.65) and ΔE00 and DC (R=-0.64). A significant positive correlation was observed mean ΔEab and ΔE00 (R=0.99). It was concluded that the different luting agents influenced the final color of the restorations. The heating of the composite resins did not affect their DC.
DOI: 10.25345/c5r761
title: MassIVE MSV000086013 - GNPS - SD Zoo - Rhino FMT - Maxis
title: Small molecule analysis of Rhino fecal extracts, standard methanol extraction. Pre and post FMT. Data were acquired using a Bruker Maxis Impact MS and C18 RP-UHPLC. Positive polarity acquisition of LC-MS/MS.
DOI: 10.6084/m9.figshare.11966364
title: Nutritional enrichment of beef burgers by adding components of non-conventional food plants

description: Abstract Some non-conventional food plants (NCFPs) such as Yacon potato, Moringa, and Ora-pro-nobis have been studied to identify their constitution and health-promoting substances. The purpose of this study was to enrich the beef burger with unconventional food plant and to evaluate its physical, chemical and sensorial properties. Ten formulations were prepared, being one formulation for the conventional burger (with soy protein and without addition of NCFPs) and nine formulations with variations of 2%, 4% and 6% of flour of each of the NCFPs. Addition of NCFPs flours improved nutritional burger properties and resulted in an increase in protein, ash, and dietary fiber content, a slight reduction in fats and significant reduction in carbohydrates. The burgers produced using 2% and 4% of Yacon flour had the lowest differences in these parameters as compared to those of the conventional burger, and also presented acceptance rates that were similar to that of the conventional burger. Both formulations showed the greatest similarity to the conventional burger during sensory analysis, which could be well accepted by consumers who are looking for healthier foods.
DOI: 10.6084/m9.figshare.11966370
title: The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression

description: Abstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.
DOI: 10.6084/m9.figshare.11966370.v1
title: The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression

description: Abstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.
DOI: 10.6084/m9.figshare.11966361
title: Apical Displacement and Residual Root Canal Filling with Single-Cone After Post Space Preparation: A Micro-CT Analysis

description: Abstract The purpose of this study was to evaluate the presence of residues after post space preparation (PSP) and establish whether the apical displacement of the gutta-percha was affected by the moments and methods of PSP in teeth filled by the single-cone technique. The root canals of 20 bovine incisor teeth were instrumented with Reciproc and filled with single-cone and AH Plus. The specimens were divided into 4 groups according to the moment and method of PSP (n=5): immediate with drill, immediate with thermoplasticizer, delayed with drill and delayed with thermoplasticizer. Micro-CT scans were performed before and after the PSP for residues of the root canal filling (1) and analysis of apical displacement (2). Data were analyzed by using 2-way repeated measurement ANOVA (1) and 2-way ANOVA (2) followed by the Tukey’s test (a=0.05). Significance effect on the residues percentage remaining for methods (p=0.044), for moments (p=0.006), for thirds repetition (p&lt;0.001), and for interaction between methods and thirds (p&lt;0.001), and moments and thirds (p=0.044). Significance effect on the apical displacement of root canal filing was detected for methods (p=0.008), however no difference was found between moments (p=0.617). In general, PSP using drill resulted in more homogeneous root canal preparation, mainly when made immediately. For all other combinations between methods and moments for PSP, the middle and apical thirds presented significant higher residues remaining. Thermo method performed in both moments and the drill method performed immediately had displacement in the apical direction, representing extrusion of the root filling material.
DOI: 10.6084/m9.figshare.11966361.v1
title: Apical Displacement and Residual Root Canal Filling with Single-Cone After Post Space Preparation: A Micro-CT Analysis

description: Abstract The purpose of this study was to evaluate the presence of residues after post space preparation (PSP) and establish whether the apical displacement of the gutta-percha was affected by the moments and methods of PSP in teeth filled by the single-cone technique. The root canals of 20 bovine incisor teeth were instrumented with Reciproc and filled with single-cone and AH Plus. The specimens were divided into 4 groups according to the moment and method of PSP (n=5): immediate with drill, immediate with thermoplasticizer, delayed with drill and delayed with thermoplasticizer. Micro-CT scans were performed before and after the PSP for residues of the root canal filling (1) and analysis of apical displacement (2). Data were analyzed by using 2-way repeated measurement ANOVA (1) and 2-way ANOVA (2) followed by the Tukey’s test (a=0.05). Significance effect on the residues percentage remaining for methods (p=0.044), for moments (p=0.006), for thirds repetition (p&lt;0.001), and for interaction between methods and thirds (p&lt;0.001), and moments and thirds (p=0.044). Significance effect on the apical displacement of root canal filing was detected for methods (p=0.008), however no difference was found between moments (p=0.617). In general, PSP using drill resulted in more homogeneous root canal preparation, mainly when made immediately. For all other combinations between methods and moments for PSP, the middle and apical thirds presented significant higher residues remaining. Thermo method performed in both moments and the drill method performed immediately had displacement in the apical direction, representing extrusion of the root filling material.