cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/s5kt6-eaj26
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-405/rawdata/</p>JV9082_20180901a0179.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-405/rawdata/JV9082_20180901a0179.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0179_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-405/3dimage_97111/JV9082_20180901a0179_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zz8dx-k9s71
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-243/rawdata/</p>Hpylori_DFliF_FISE_237.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-243/rawdata/Hpylori_DFliF_FISE_237.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_237_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-243/3dimage_113968/Hpylori_DFliF_FISE_237_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0qc3k-w3s77
title: D. flos-aquae Gas Vesicles

description: Gas vesicles purified from D. flos-aquae, WT
description: <strong>Tilt Series Date:</strong> 2020-11-04</p>
<strong>Data Taken By:</strong> Przemek Dutka</p>
<strong>Species / Specimen:</strong> Dolichospermum flos-aquae</p>
<strong>Strain:</strong> CCAP 1403/13F</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: 2.5 μm,  magnification: 64000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> </p>
<strong>Collaborators and Roles:</strong> purified by Dina Malounda the Shapiro lab; data collected by Przemek . Dutka and Songye Chen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Lakshmanan, A., Lu, G., Farhadi, A. et al. Preparation of biogenic gas . vesicle nanostructures for use as contrast agents for ultrasound and . MRI. Nat Protoc 12, 2050–2080 (2017). . https://doi.org/10.1038/nprot.2017.081. Sample in 10mM Hepes, pH7.4; OD 5.0</p>
<strong>Sample Preparation:</strong> Quantifoil C2/2 200mesh extra thick carbon, 10nm BSA-gold, 100% . humidity, Blot time 4, Blot force 3, temp 4C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-7/rawdata/</p>AnaGVOD5_12_movies.mrc,  Tilt Series (Pixel Size 0.14 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-7/rawdata/AnaGVOD5_12_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        AnaGVOD5_12.mrc.mdoc,  26.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-7/rawdata/AnaGVOD5_12.mrc.mdoc"
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DOI: 10.22002/d4994-78665
title: P_aeruginosa_PA14_DfliF_Krios

description: Raw data files of P_aeruginosa_PA14_DfliF_Krios
description: <strong>Tilt Series Date:</strong> 2018-09-29</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (50.0°, -50.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> I (mohammed) obtained the cells from Shannon Park (Dianne Newman lab). The cells are available in their library of Pseudomonas aeruginosa PA14.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated one colony from an LB agar plate and grew the cells overnight in LB with 100 ug/ml of Gentamycin. Then I concentrated the cells three times and mixed them with gold nanoparticles and added 3 ul on to thick carbon R2 grids and blotted them and plunge froze them (force either 6 or 3, time either 3 or 2 seconds respectively, drain time 1 second).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-22/rawdata/</p>Pseudomonas_DfliF23.mrc,  Tilt Series (Pixel Size 0.53 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-22/rawdata/Pseudomonas_DfliF23.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudomonas_DfliF23_full.rec,  Reconstruction (Pixel Size 2.12 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-22/3dimage_97170/Pseudomonas_DfliF23_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wdnjm-gkg33
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-428/rawdata/</p>Hpylori_DFliF_FISE_433.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-428/rawdata/Hpylori_DFliF_FISE_433.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_433_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-428/3dimage_114324/Hpylori_DFliF_FISE_433_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ma5ex-byb06
title: JV9082_K3_janelia

description: JV9082:DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2019-12-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -2.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-80/rawdata/</p>9082_Box1_No1_S100.mrc,  Tilt Series (Pixel Size 0.261 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-80/rawdata/9082_Box1_No1_S100.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_Box1_No1_S100_full.rec,  Reconstruction (Pixel Size 1.044 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-80/3dimage_112619/9082_Box1_No1_S100_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.kaf34p
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Galaxias zebratus (Castelnau, 1861)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((15.4784 -23.4046,15.4933 -24.0715,20.509 -24.0715,20.509 -24.0455,20.0952 -23.5072,19.9968 -23.017,19.7843 -22.5405,19.1988 -21.8785,18.5972 -21.5447,17.8859 -21.3993,17.1626 -21.4805,16.5332 -21.7596,16.1257 -22.0882,15.8234 -22.4662,15.606 -22.8993,15.4784 -23.4046))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0022532-250525065834625/datasets/export for details.
DOI: 10.15468/dl.s4xaae
title: Occurrence Download

description: A dataset containing 2511 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Diaphorina citri Kuwayama, 1908, Trioza erytreae (Del Guercio, 1918))"
 ]
}

 The dataset includes 2511 records from 28 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0022533-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/whe67-p8e72
title: MJ sacculi

description: Skinny B. subtilis MJ007 sacculi of sporulating cells
description: <strong>Tilt Series Date:</strong> 2012-03-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kit Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> see Mol Micro Tocheva et al reference</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-10/rawdata/</p>mjsac20010.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-10/rawdata/mjsac20010.mrc"
        > <i class="download icon"></i> Download </a></p>

        mjsac20010_full.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-10/3dimage_17359/mjsac20010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nz9k2-73x10
title: Pseudoalteromonas Iuteoviolacea MP121

description: P Iuteo DvipABhcpI Dbact2 BP-sfGFP
description: <strong>Tilt Series Date:</strong> 2013-06-22</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> on plate</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-22-16/rawdata/</p>2013_06_22_mp121_0023.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-06-22-16/rawdata/2013_06_22_mp121_0023.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_06_22_mp121_0023_2k_full.rec,  Reconstruction (Pixel Size 1.97 nm), 801.8 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.yt8ust
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Aplocheilichthys lacustris, Lacustricola lacustris, Aplocheilichthys macrurus, Lacustricola macrurus, Aplocheilichthys maculatus, Lacustricola maculatus, Aplocheilichthys mediolateralis, Lacustricola mediolateralis, Aplocheilichthys nigrolateralis, Lacustricola nigrolateralis, Aplocheilichthys omoculatus, Lacustricola omoculatus, Aplocheilichthys rudolfianus, Micropanchax rudolfianus, Aplocheilichthys spilauchen, Aplocheilichthys usanguensis, Lacustricola usanguensis, Aplocheilichthys vitschumbaensis, Lacustricola vitschumbaensis, Aplocheilus panchax, Apricaphanius saourensis, Archiaphyosemion guineense, Aristochromis christyi, Arius africanus, Arius festinus, Arius gigas, Arius latiscutatus, Arius madagascariensis, Arius uncinatus, Arnoglossus imperialis, Arnoldichthys spilopterus, Asprotilapia leptura, Xenotilapia leptura, Astatoreochromis alluaudi, Astatoreochromis straeleni, Astatoreochromis vanderhorsti, Astatotilapia bloyeti, Astatotilapia burtoni, Astatotilapia calliptera, Astatotilapia desfontainii, Astatotilapia paludinosa, Haplochromis paludinosus, Astatotilapia stappersii, Astatotilapia tweddlei, Atherina boyeri, Atherina caspia, Atherinomorus duodecimalis, Atopochilus chabanaudi, Atopochilus christyi, Atopochilus macrocephalus, Atopochilus mandevillei, Atopochilus pachychilus, Atopochilus savorgnani, Atopochilus vogti, Atopodontus adriaensi, Auchenoglanis biscutatus, Auchenoglanis occidentalis, Aulonocara aquilonium, Aulonocara auditor, Aulonocara baenschi, Aulonocara brevinidus, Aulonocara brevirostre, Aulonocara ethelwynnae, Aulonocara gertrudae, Aulonocara guentheri, Aulonocara hueseri, Aulonocara jacobfreibergi, Aulonocara kandeense, Aulonocara kandeensis, Aulonocara koningsi, Aulonocara korneliae, Aulonocara maylandi, Aulonocara nyassae, Aulonocara rostratum, Aulonocara saulosi, Aulonocara stonemani, Aulonocara stuartgranti, Aulonocara trematocephalum, Aulonocranus dewindti, Aurigequula fasciata, Auriglobus modestus, Austroglanis barnardi, Austroglanis gilli, Austroglanis sclateri, Awaous aeneofuscus, Awaous bustamantei, Awaous commersoni, Awaous lateristriga, Awaous macrorhynchus, Bagrus bajad, Bagrus caeruleus, Bagrus degeni, Bagrus docmak, Bagrus filamentosus, Bagrus lubosicus, Bagrus meridionalis, Bagrus orientalis, Bagrus ubangensis, Bagrus urostigma)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((59.2345 -15.8834,59.2192 -15.9401,59.9717 -15.9401,59.949 -15.8852,59.5935 -15.7371,59.2345 -15.8834))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005872-250827131500795/datasets/export for details.
DOI: 10.22002/e10w1-my293
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-34/3dimage_68915/</p>12jan06_c105_1_4ADNCos7_AT3man1_full.rec,  Reconstruction (Pixel Size 2.0 nm), 858.8 MB  
        
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        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3man1.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8wr6t-z0s44
title: gold labeled CheA- in vitro

description: post wash sample - gold labeled CheA, 1.8 nm gold, post ternary formation snap rxn.
description: <strong>Tilt Series Date:</strong> 2013-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Heather Hodge and Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Buffer: 1x Snap + 1mM DTT 50 mM Tris Hcl pH 7.5, 100 mM Ncl, 0.1% Tween-200)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-82/rawdata/</p>HTH1B_10036.mrc,  Tilt Series (Pixel Size 0.2989 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-82/rawdata/HTH1B_10036.mrc"
        > <i class="download icon"></i> Download </a></p>

        HTH1B_10036_part67_2.rec,  Reconstruction (Pixel Size 0.5978 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-82/3dimage_26525/HTH1B_10036_part67_2.rec"
        > <i class="download icon"></i> Download </a></p>