cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.2068944.v1
title: Systematic consideration of floral microcharacters of the South American genus <i>Chrysolaena</i> (Vernonieae, Asteraceae)

description: Floral microcharacters of the genus <i>Chrysolaena</i> H. Rob. (Vernonieae, Asteraceae) were analysed in detail for the first time in order to evaluate the taxonomic position of conflictive species in the group. The results were also compared with studies carried out in species of related genera. In addition to distinctive microcharacters previously studied in some species of the genus, other characters such as trichome types of the corolla, style, anthers and cypselae have been analysed for the first time. The presence of glandular apical appendage and cypselae are common characteristics among species <i>Chrysolaena</i>. In addition to these, this study shows that presence of glands on the style and corollas is another consistent characteristic in the genus. However, the absence of basal stylar node would not be a diagnostic character since this varies widely among species. The results indicate that most of the microcharacters of <i>Chrysolaena</i> analysed are quite consistent in the genus, but they are no more consistent than the pollen morphology (type 'C') and chromosome base number (<i>x</i> = 10). Until now, these last two features would be most useful for separating <i>Chrysolaena</i> from the related genera <i>Lessingianthus</i> and <i>Lepidaploa</i>. At species level, the results show that related species can be distinguished by the different combinations of floral microcharacters. The value of microcharacters could be increased if they are combined with other morphological, cytological, and palynological data.
DOI: 10.6084/m9.figshare.2068851
title: A psychometric evaluation of the Gender Bias in Medical Education Scale

description: <em><b>Background: </b></em><em>Gender </em>bias in medical education is an increasing area of research and policy concern. However, to adequately address and monitor this issue, valid and reliable measures are needed. This research aimed to develop and test a short multidimensional measure of gender bias in medical education to assess student awareness of gender bias, beliefs about the degree to which gender bias should be addressed, and experience of gender bias. <em><b>Methods: </b></em>Using students from the School of Medicine at the University of Wollongong, one pilot study and two empirical studies were conducted. The pilot study was used to scope the domain space (n = 28). This initial measure was extended to develop the Gender Bias in Medical Education Scale (GBMES). For study 1 (n = 172), confirmatory factor analysis assessed the construct validity of the three-factor structure (awareness, beliefs, experience) and enabled deletion of redundant items. Study 2 (n = 457) tested the generalizability of the refined scale to a new sample. Combining study 1 and 2, invariance testing for program of study and gender was explored. The relationship of the GBMES to demographic and gender politics variables was tested. The results were analyzed using confirmatory factor analysis and Multiple-Indicator-Multiple-Indicator-Cause models in order to determine construct validity and whether the scale generalized to other samples. <em><b>Results:</b></em><i> </i>After analysis of the responses from the original 16-item GBMES (Study 1), a shortened measure of 10 items fitted the data well and was found to generalize to a new sample in Study 2. The GBMES was found to be invariant across studies, gender, and program of study. Female students and those who reported greater agreement with gender equality responded with greater agreement for each of the factors in relation to gender bias. Likewise, postgraduate students reported higher scores on experience of gender bias than undergraduate students. <b></b> <b><i>Conclusion:</i></b><i> </i>The GBMES provides a validated short multidimensional measure for use in research and policy. The factors are distinct and are related to background variables and covariates in expected patterns.<b></b>
DOI: 10.6084/m9.figshare.2068842
title: Translational regulation of the mRNA encoding the ubiquitin peptidase USP1 involved in the DNA damage response as a determinant of Cisplatin resistance

description: Cisplatin (cis-diaminedichloroplatin (II), CDDP) is part of the standard therapy for a number of solid tumors including Non-Small-Cell Lung Cancer (NSCLC). The initial response observed is in most cases only transient and tumors quickly become refractory to the drug. Tumor cell resistance to CDDP relies on multiple mechanisms, some of which still remain unknown. In search for such mechanisms, we examined the impact of CDDP on mRNA translation in a sensitive and in a matched resistant NSCLC cell line. We identified a set of genes whose mRNAs are differentially translated in CDDP resistant vs. sensitive cells. The translation of the mRNA encoding the Ubiquitin-Specific Peptidase 1 (USP1), a Ubiquitin peptidase with important function in multiple DNA repair pathways, is inhibited by CDDP exposure in the sensitive cells, but not in the resistant cells. This lack of down-regulation of USP1 expression at the translational level plays a primary role in CDDP resistance since inhibition of USP1 expression or activity by siRNA or the small molecule inhibitor ML323, respectively is sufficient to re-sensitize resistant cells to CDDP. We involved the USP1 mRNA translation as a major mechanism of CDDP resistance in NSCLC cells and suggest that USP1 could be evaluated as a candidate predictive marker and as a therapeutic target to overcome CDDP resistance. More generally, our results indicate that analysis of gene expression at the level of mRNA translation is a useful approach to identify new determinants of CDDP resistance.
DOI: 10.6084/m9.figshare.2068839
title: E2f4 and E2f5 are essential for the development of the male reproductive system

description: The E2F transcription factors are primarily implicated in the regulation of entry and exit from the cell cycle. However, <i>in vivo</i> studies have established additional roles for E2Fs during organ development and homeostasis. With the goal of addressing the intestinal requirements of <i>E2f4</i> and <i>E2f5</i>, we crossed mice carrying <i>Vil-cre, E2f4</i> conditional and <i>E2f5</i> germline alleles. <i>E2f4</i> deletion had no detectable effect on intestinal development. However, <i>E2f4f/f;E2f5+/−;Vil-cre</i> males, but not <i>E2f4f/f;Vil-cre</i> littermates, were unexpectedly sterile. This defect was not due to defective spermatogenesis. Instead, the seminiferous tubules and rete testes showed significant dilation, and spermatozoa accumulated aberrantly in the rete testis and efferent ducts. Our data show that these problems result from defective efferent ducts, a tissue whose primary function is to concentrate sperm through fluid absorption. First, <i>Vil-cre</i> expression, and consequent E2F4 loss, was specific to the efferent ducts and not other reproductive tract tissues. Second, the <i>E2f4f/f;E2f5+/−;Vil-cre</i> efferent ducts had completely lost multiciliated cells and greatly reduced levels of critical absorptive cell proteins: aquaporin1, a water channel protein, and clusterin, an endocytic marker. Collectively, the observed testis phenotypes suggest a fluid flux defect. Remarkably, we observed rete testis dilation prior to the normal time of seminiferous fluid production, arguing that the efferent duct defects promote excessive secretory activity within the reproductive tract. Finally, we also detect key aspects of these testis defects in <i>E2f5</i>−/− mice. Thus, we conclude that <i>E2f4</i> and <i>E2f5</i> display overlapping roles in controlling the normal development of the male reproductive system.
DOI: 10.6084/m9.figshare.2068842.v1
title: Translational regulation of the mRNA encoding the ubiquitin peptidase USP1 involved in the DNA damage response as a determinant of Cisplatin resistance

description: Cisplatin (cis-diaminedichloroplatin (II), CDDP) is part of the standard therapy for a number of solid tumors including Non-Small-Cell Lung Cancer (NSCLC). The initial response observed is in most cases only transient and tumors quickly become refractory to the drug. Tumor cell resistance to CDDP relies on multiple mechanisms, some of which still remain unknown. In search for such mechanisms, we examined the impact of CDDP on mRNA translation in a sensitive and in a matched resistant NSCLC cell line. We identified a set of genes whose mRNAs are differentially translated in CDDP resistant vs. sensitive cells. The translation of the mRNA encoding the Ubiquitin-Specific Peptidase 1 (USP1), a Ubiquitin peptidase with important function in multiple DNA repair pathways, is inhibited by CDDP exposure in the sensitive cells, but not in the resistant cells. This lack of down-regulation of USP1 expression at the translational level plays a primary role in CDDP resistance since inhibition of USP1 expression or activity by siRNA or the small molecule inhibitor ML323, respectively is sufficient to re-sensitize resistant cells to CDDP. We involved the USP1 mRNA translation as a major mechanism of CDDP resistance in NSCLC cells and suggest that USP1 could be evaluated as a candidate predictive marker and as a therapeutic target to overcome CDDP resistance. More generally, our results indicate that analysis of gene expression at the level of mRNA translation is a useful approach to identify new determinants of CDDP resistance.
DOI: 10.6084/m9.figshare.2068839.v1
title: E2f4 and E2f5 are essential for the development of the male reproductive system

description: The E2F transcription factors are primarily implicated in the regulation of entry and exit from the cell cycle. However, <i>in vivo</i> studies have established additional roles for E2Fs during organ development and homeostasis. With the goal of addressing the intestinal requirements of <i>E2f4</i> and <i>E2f5</i>, we crossed mice carrying <i>Vil-cre, E2f4</i> conditional and <i>E2f5</i> germline alleles. <i>E2f4</i> deletion had no detectable effect on intestinal development. However, <i>E2f4f/f;E2f5+/−;Vil-cre</i> males, but not <i>E2f4f/f;Vil-cre</i> littermates, were unexpectedly sterile. This defect was not due to defective spermatogenesis. Instead, the seminiferous tubules and rete testes showed significant dilation, and spermatozoa accumulated aberrantly in the rete testis and efferent ducts. Our data show that these problems result from defective efferent ducts, a tissue whose primary function is to concentrate sperm through fluid absorption. First, <i>Vil-cre</i> expression, and consequent E2F4 loss, was specific to the efferent ducts and not other reproductive tract tissues. Second, the <i>E2f4f/f;E2f5+/−;Vil-cre</i> efferent ducts had completely lost multiciliated cells and greatly reduced levels of critical absorptive cell proteins: aquaporin1, a water channel protein, and clusterin, an endocytic marker. Collectively, the observed testis phenotypes suggest a fluid flux defect. Remarkably, we observed rete testis dilation prior to the normal time of seminiferous fluid production, arguing that the efferent duct defects promote excessive secretory activity within the reproductive tract. Finally, we also detect key aspects of these testis defects in <i>E2f5</i>−/− mice. Thus, we conclude that <i>E2f4</i> and <i>E2f5</i> display overlapping roles in controlling the normal development of the male reproductive system.
DOI: 10.6084/m9.figshare.2068821
title: Main Data for Clinical Trials Registry use by Systematic reviews and Meta-analyses in Pregnancy and Childbirth Journals

description: This data represents the coding results for the top 20 Journals in the Pregnancy and Childbirth literature. A 1 indicates that a systematic review or meta-analysis searched clinical trials registry, while a 0 indicates that the trials registry was not searched. The columns represent the clinical trials registries that were searched, while individual rows are representative of the study in question (except for the sum rows).
DOI: 10.6084/m9.figshare.2068830
title: Mitotic phosphatase activity is required for MCC maintenance during the spindle checkpoint

description: The spindle checkpoint prevents activation of the anaphase-promoting complex (APC/C) until all chromosomes are correctly attached to the mitotic spindle. Early in mitosis, the mitotic checkpoint complex (MCC) inactivates the APC/C by binding the APC/C activating protein CDC20 until the chromosomes are properly aligned and attached to the mitotic spindle, at which point MCC disassembly releases CDC20 to activate the APC/C. Once the APC/C is activated, it targets cyclin B and securin for degradation, and the cell progresses into anaphase. While phosphorylation is known to drive many of the events during the checkpoint, the precise molecular mechanisms regulating spindle checkpoint maintenance and inactivation are still poorly understood. We sought to determine the role of mitotic phosphatases during the spindle checkpoint. To address this question, we treated spindle checkpoint-arrested cells with various phosphatase inhibitors and examined the effect on the MCC and APC/C activation. Using this approach we found that 2 phosphatase inhibitors, calyculin A and okadaic acid (1 μM), caused MCC dissociation and APC/C activation leading to cyclin A and B degradation in spindle checkpoint-arrested cells. Although the cells were able to degrade cyclin B, they did not exit mitosis as evidenced by high levels of Cdk1 substrate phosphorylation and chromosome condensation. Our results provide the first evidence that phosphatases are essential for maintenance of the MCC during operation of the spindle checkpoint.
DOI: 10.6084/m9.figshare.2068821.v1
title: Main Data for Clinical Trials Registry use by Systematic reviews and Meta-analyses in Pregnancy and Childbirth Journals

description: This data represents the coding results for the top 20 Journals in the Pregnancy and Childbirth literature. A 1 indicates that a systematic review or meta-analysis searched clinical trials registry, while a 0 indicates that the trials registry was not searched. The columns represent the clinical trials registries that were searched, while individual rows are representative of the study in question (except for the sum rows).
DOI: 10.6084/m9.figshare.2068830.v1
title: Mitotic phosphatase activity is required for MCC maintenance during the spindle checkpoint

description: The spindle checkpoint prevents activation of the anaphase-promoting complex (APC/C) until all chromosomes are correctly attached to the mitotic spindle. Early in mitosis, the mitotic checkpoint complex (MCC) inactivates the APC/C by binding the APC/C activating protein CDC20 until the chromosomes are properly aligned and attached to the mitotic spindle, at which point MCC disassembly releases CDC20 to activate the APC/C. Once the APC/C is activated, it targets cyclin B and securin for degradation, and the cell progresses into anaphase. While phosphorylation is known to drive many of the events during the checkpoint, the precise molecular mechanisms regulating spindle checkpoint maintenance and inactivation are still poorly understood. We sought to determine the role of mitotic phosphatases during the spindle checkpoint. To address this question, we treated spindle checkpoint-arrested cells with various phosphatase inhibitors and examined the effect on the MCC and APC/C activation. Using this approach we found that 2 phosphatase inhibitors, calyculin A and okadaic acid (1 μM), caused MCC dissociation and APC/C activation leading to cyclin A and B degradation in spindle checkpoint-arrested cells. Although the cells were able to degrade cyclin B, they did not exit mitosis as evidenced by high levels of Cdk1 substrate phosphorylation and chromosome condensation. Our results provide the first evidence that phosphatases are essential for maintenance of the MCC during operation of the spindle checkpoint.
DOI: 10.6084/m9.figshare.2068797
title: DO NOT BE MOTHER RIGHT: REFLECTIONS ON THE LEGAL DELIVER WOMAN RIGHT TO SON TO ADOPTION

description: La sociedade industrial ha traído valores que alejan las mujeres de aquillo que llamamos de “destino biológico”. Tal hecho cuando valorado con otros temas que encuentram su fundamentación en las garantias de los derechos a todos los ciudadanos, ayudan a generar una cantidad infinita de ideas bajo el discurso de la libertad sexual de la mujer y, aún, hacia el discurso feminista a que todos estan sometidos en tempos actuales, promocionan, aún un nuevo concepto de lo que se reconosce como família. Esa família de la modernidade, por lo tanto, no más puede estar fundamentada en valores como el matrimonio, una vez que se considera en razón de valores individuales como son ejemplo el afeto y el amor. Al reconoscer la família como un valor afectivo, es necessário pensar sobre el derecho de toda la mujer en elejir o no elejir por lo que se llama de maternagen mientras no estar acusada de abandono. De hecho, el tema proponido tiene un ancho debate de caracter sócio-politico. Sin embargo, el trabajo considera necessário algunas reflexiones del tema família. Pero, si destina a la discusión de las reflexiones jurídicas sobre el derecho de entregar el hijo para la adopción.<br>
DOI: 10.6084/m9.figshare.2068797.v1
title: DO NOT BE MOTHER RIGHT: REFLECTIONS ON THE LEGAL DELIVER WOMAN RIGHT TO SON TO ADOPTION

description: La sociedade industrial ha traído valores que alejan las mujeres de aquillo que llamamos de “destino biológico”. Tal hecho cuando valorado con otros temas que encuentram su fundamentación en las garantias de los derechos a todos los ciudadanos, ayudan a generar una cantidad infinita de ideas bajo el discurso de la libertad sexual de la mujer y, aún, hacia el discurso feminista a que todos estan sometidos en tempos actuales, promocionan, aún un nuevo concepto de lo que se reconosce como família. Esa família de la modernidade, por lo tanto, no más puede estar fundamentada en valores como el matrimonio, una vez que se considera en razón de valores individuales como son ejemplo el afeto y el amor. Al reconoscer la família como un valor afectivo, es necessário pensar sobre el derecho de toda la mujer en elejir o no elejir por lo que se llama de maternagen mientras no estar acusada de abandono. De hecho, el tema proponido tiene un ancho debate de caracter sócio-politico. Sin embargo, el trabajo considera necessário algunas reflexiones del tema família. Pero, si destina a la discusión de las reflexiones jurídicas sobre el derecho de entregar el hijo para la adopción.<br>
DOI: 10.6084/m9.figshare.2068743
title: The Pups.jpg

description: Portrait - 2 PWD
DOI: 10.6084/m9.figshare.2068737
title: Structure and dynamics of edge flames in the near wake of unequal merging shear flows

description: We examine in this study the structure and dynamic properties of an edge flame formed in the near-wake of two initially separated shear flows, one containing fuel and the other oxidiser. A comprehensive study is carried out within the diffusive-thermal framework where the flow field, computed a-priori, is used for the determination of the combustion field. Our focus is on the effects of three controlling parameters: the Damköhler number controlling the overall flow rate, the oxidiser-to-fuel strain rate ratio of the supply streams that determines the extent of oxidiser entrainment towards the mixing zone, and the Lewis number, assumed equal for the fuel and oxidiser, that depends on the mixture composition. Response curves, representing the edge flame standoff distance as a function of Damköhler number, exhibit two distinct shapes: C-shaped and U-shaped curves characterising the response of low and high Lewis number flames, respectively. Stability considerations show that the upper solution branch of the C-shaped response curve is unstable and hence corresponds to physically unrealistic states, but due to heat conduction toward the cold plate the lower solution branch is always stable. The states forming this solution branch correspond to flame attachment, where the edge flame remains practically attached to the tip of the plate until it is blown off by the flow when the velocity exceeds a critical value. The U-shaped response, on the other hand, consists of equilibrium states that are globally stable. Thus, high Lewis number flames can be always stabilised near the splitter plate, with the edge held stationary or undergoing a back and forth motion, or lifted and stabilised downstream by the flow. Insight into the distinct stabilisation characteristics, exhibited by the different Lewis number cases, is given by examining the relationship between the local flow velocity and the edge propagation speed.
DOI: 10.6084/m9.figshare.2068743.v1
title: The Pups.jpg

description: Portrait - 2 PWD
DOI: 10.6084/m9.figshare.2068737.v1
title: Structure and dynamics of edge flames in the near wake of unequal merging shear flows

description: We examine in this study the structure and dynamic properties of an edge flame formed in the near-wake of two initially separated shear flows, one containing fuel and the other oxidiser. A comprehensive study is carried out within the diffusive-thermal framework where the flow field, computed a-priori, is used for the determination of the combustion field. Our focus is on the effects of three controlling parameters: the Damköhler number controlling the overall flow rate, the oxidiser-to-fuel strain rate ratio of the supply streams that determines the extent of oxidiser entrainment towards the mixing zone, and the Lewis number, assumed equal for the fuel and oxidiser, that depends on the mixture composition. Response curves, representing the edge flame standoff distance as a function of Damköhler number, exhibit two distinct shapes: C-shaped and U-shaped curves characterising the response of low and high Lewis number flames, respectively. Stability considerations show that the upper solution branch of the C-shaped response curve is unstable and hence corresponds to physically unrealistic states, but due to heat conduction toward the cold plate the lower solution branch is always stable. The states forming this solution branch correspond to flame attachment, where the edge flame remains practically attached to the tip of the plate until it is blown off by the flow when the velocity exceeds a critical value. The U-shaped response, on the other hand, consists of equilibrium states that are globally stable. Thus, high Lewis number flames can be always stabilised near the splitter plate, with the edge held stationary or undergoing a back and forth motion, or lifted and stabilised downstream by the flow. Insight into the distinct stabilisation characteristics, exhibited by the different Lewis number cases, is given by examining the relationship between the local flow velocity and the edge propagation speed.
DOI: 10.6084/m9.figshare.2068695
title: AFS_data_analyse_170323 -labview 2016

description: analyse software