DOI: 10.25338/b80w73
title: Data for: A mosquito parasite is locally adapted to its host but not temperature

description: Climate change will alter interactions between parasites and their hosts.
 Warming may affect patterns of local adaptation, shifting the environment
 to favor the parasite or host and thus changing the prevalence of disease.
 We assessed local adaptation to hosts and temperature in the facultative
 ciliate parasite Lambornella clarki, which infects the western tree hole
 mosquito Aedes sierrensis. We conducted laboratory infection experiments
 with mosquito larvae and parasites collected from across a climate
 gradient, pairing sympatric or allopatric populations across three
 temperatures that were either matched or mismatched to the source
 environment. L. clarki parasites were locally adapted to their hosts, with
 2.6x higher infection rates on sympatric compared to allopatric
 populations, but were not locally adapted to temperature. Infection peaked
 at the intermediate temperature of 12.5°C, notably lower than the optimum
 temperature for free-living L. clarki growth, suggesting that the host’s
 immune response can play a significant role in mediating the outcome of
 infection. Our results highlight the importance of host selective pressure
 on parasites, despite the impact of temperature on infection success.
description: # Data for "A mosquito parasite is locally adapted to its host but
 not temperature" Paper associated with this data archive: "A
 mosquito parasite is locally adapted to its host but not temperature"
 Authors: Kelsey Lyberger corresponding author
 [klyberger@stanford.edu](mailto:klyberger@stanford.edu) Johannah Farner
 [jfarner@stanford.edu](mailto:jfarner@stanford.edu) Lisa Couper
 [lcouper@stanford.edu](mailto:lcouper@stanford.edu) Erin A Mordecai
 [emordeca@stanford.edu](mailto:emordeca@stanford.edu) Citation: Lyberger,
 K, F Farner, L Couper, and E A Mordecai. 2024. A mosquito parasite is
 locally adapted to its host but not temperature. American Naturalist,
 Dryad Digital Repository,
 [https://doi.org/10.25338/B80W73](https://doi.org/10.25338/B80W73).
 Funding Sources: This work was supported by the NSF Postdoctoral Research
 Fellowships in Biology Program under Grant No. 2208947. EAM was supported
 by the National Institutes of Health (R35GM133439, R01AI168097,
 R01AI102918), the National Science Foundation (DEB-2011147, with Fogarty
 International Center), and the Stanford Center for Innovation in Global
 Health, King Center on Global Development, and Woods Institute for the
 Environment. LC was funded by the Philippe Cohen Graduate Fellowship and
 the Stanford Center for Computational, Evolutionary and Human Genomics. JF
 was funded by the Bing-Mooney Fellowship. Description of the data: There
 are four datasets. Two are data from the growth experiment of Lambornella
 clarki grown in culture at different temperatures. The third is data from
 the infection experiment of Lambornella clarki into Aedes sierrensis
 mosquitoes. The fourth is the distance between populations measured in
 kilometers. ## Description of the data and file structure There are four
 data files and two code files. "GxE_lambo_alone_AN.csv" This
 file contains data from the growth experiment. "maxPop.csv" This
 file contains data on maximum growth rates from the growth experiment.
 "GxE_data_infection_AN.csv" This file contains data from the
 infection experiment. "GxE_distance_AN.csv" This file contains
 data on the distance between populations. NA represents that the field was
 not applicable. In column "notes" this means there was nothing
 important to note down. In the columns "cell_count_1" to
 "cell_count_5" this means there were no cells to count, i.e.,
 when the larvae was not infected. Column headings:
 "GxE_lambo_alone_AN.csv" Day - day of the experiment (1-6) Color
 - the color refers to the Lambornella population, the translation to the 3
 letter codes in the text are given in GxE Lambo thermal performance.R
 Replicate - replicate (1-5) 5C, 7C, 12C, 18C, 21C, 23C, 28C -these columns
 refer to the temperatures at which the cultures were incubated, recorded
 are the number of cells counted per 100ul subsample.
 "maxPop.csv" maxPop - the maximum growth rate of the free-living
 Lambornella clarki population listpop - the Lambornella clarki population
 "GxE_data_infection_AN.csv" Date - Calendar date Day - day
 checked Tray - ID for 6-well falcon plate Temperature - temperature in
 degrees C Aedes_pop - Aedes sierrensis population Lambo_pop - Lambornella
 clarki population Rep - replicate num_encysted - number of larvae with
 cysts num_survived - number of larvae survived num_melanized - number of
 larvae with melanization spots Lambo_presence - free-living Lambornella
 present in the water notes - anything of note cell_count_1 to cell_count_5
 - the number of cells of Lambornella inside each of the 5 larvae.
 "GxE_distance_AN.csv" Aedes_pop - the Aedes sierrensis
 population Lambo_pop - the Lambornella clarki population Distance_mi - the
 distance in miles between the two populations Distance_km - the distance
 in kilometers between the two populations Distance_temp - the difference
 in degrees C between the mean annual temperatures of the two populations.
 ## Sharing/Access information This data was collected by Kelsey Lyberger.
 Please contact [klyberger@gmail.com](mailto:klyberger@gmail.com) if there
 are any questions. ## Code GxE_Lambo_thermal_performance_AN.R This code
 runs the analysis and creates the figures for the Lambornella growth
 experiment. GxE_final_analysis_AN.R This code runs the analysis and
 creates the figures for the infection experiment. Code was run using R
 version 4.1.1 Below is a list of all R packages used and their versions.
 rTPC_1.0.1 nls.multstart_1.2.0 broom_1.0.1 dplyr_1.1.2 purrr_1.0.1
 tidyr_1.2.1 ggplot2_3.4.0 boot_1.3-28 car_3.1-0 cowplot_1.1.1
 minpack.lm_1.2-1 RColorBrewer_1.1-2 ggpubr_0.4.0