cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11313173
title: Virulence factors of Candida species from the oral mucosa and prostheses of elderly people from a riverside community in the Amazon state, Brazil

description: Abstract Introduction Candida albicans is the yeast most commonly affecting the oral cavity, sometimes causing infection. However, several factors may be associated with the onset of candidiasis, which may be related not only to the hygiene and health of individuals, but also to the pathogenicity of these microorganisms. Objective To evaluate the virulence factors of Candida yeasts isolated from the oral mucosa of elderly people living in the “Comunidade Lago do Limão”, municipality of Iranduba, Amazonas state, Brazil. Material and method Testes were performed to assess the production of urease, proteinase, phospholipase and hemolysin. Statistical analysis used the Fisher's exact test and the Chi-squared test. Result Prevalence of non-albicans species was observed. As for virulence factors, all isolates were negative ureases, and there was prevalence of very strong proteinase production, whereas most isolates did not produce this enzyme in the phospholipase test. All yeasts analyzed presented hemolysin production, with grade IV hemolysis as the most prevalent. There was no statistically significant difference between the virulence of isolates from the oral cavity and the prostheses of the elderly analyzed. Conclusion Several virulence factors may present with high intensity in the presence of oral microbiota changes. In addition, non-albicans species present number of virulence factors similar to that of C. albicans, with high pathogenicity. This study allows a better analysis of candidiasis prevention strategies aiming to promote improvement in the health and quality of life for the elderly.
DOI: 10.6084/m9.figshare.11313173.v1
title: Virulence factors of Candida species from the oral mucosa and prostheses of elderly people from a riverside community in the Amazon state, Brazil

description: Abstract Introduction Candida albicans is the yeast most commonly affecting the oral cavity, sometimes causing infection. However, several factors may be associated with the onset of candidiasis, which may be related not only to the hygiene and health of individuals, but also to the pathogenicity of these microorganisms. Objective To evaluate the virulence factors of Candida yeasts isolated from the oral mucosa of elderly people living in the “Comunidade Lago do Limão”, municipality of Iranduba, Amazonas state, Brazil. Material and method Testes were performed to assess the production of urease, proteinase, phospholipase and hemolysin. Statistical analysis used the Fisher's exact test and the Chi-squared test. Result Prevalence of non-albicans species was observed. As for virulence factors, all isolates were negative ureases, and there was prevalence of very strong proteinase production, whereas most isolates did not produce this enzyme in the phospholipase test. All yeasts analyzed presented hemolysin production, with grade IV hemolysis as the most prevalent. There was no statistically significant difference between the virulence of isolates from the oral cavity and the prostheses of the elderly analyzed. Conclusion Several virulence factors may present with high intensity in the presence of oral microbiota changes. In addition, non-albicans species present number of virulence factors similar to that of C. albicans, with high pathogenicity. This study allows a better analysis of candidiasis prevention strategies aiming to promote improvement in the health and quality of life for the elderly.
DOI: 10.6084/m9.figshare.6967655
title: Extensive ameloblastoma in young patient: 5-year follow-up with no recurrence using conservative treatment

description: ABSTRACT Ameloblastoma is a benign, odontogenic tumor that, due to its low rate of incidence, slow growth and local invasiveness, can be treated with a variety of surgical approaches, ranging from conservative to radical procedures. The conventional variant of ameloblastoma, though, is more aggressive and common, presenting a higher rate of recurrence than the unicystic and extraosseous/peripheral types; usually, the treatment of choice for this variant employs more invasive procedures. This report is of a 13-year-old male patient who presented with a swelling on the posterior mandibular region, on the left side of his face. Intraoral examination revealed lingual displacement of teeth 36 and 37. The clinical, radiographic and histopathological analyses confirmed the diagnosis of ameloblastoma (plexiform histological type). The patient was treated with curettage and peripheral osteotomy and a 5-year follow-up examination showed the area to have healed completely, with no evidence of recurrence. Consequently, although the clinical management of ameloblastoma is often based on invasive surgical approaches, large tumors treated with conservative surgery are less aesthetically and functionally impaired, and may demonstrate good prognosis with no recurrence in the 5-year follow-up period, as in the case reported here.
DOI: 10.6084/m9.figshare.6967655.v1
title: Extensive ameloblastoma in young patient: 5-year follow-up with no recurrence using conservative treatment

description: ABSTRACT Ameloblastoma is a benign, odontogenic tumor that, due to its low rate of incidence, slow growth and local invasiveness, can be treated with a variety of surgical approaches, ranging from conservative to radical procedures. The conventional variant of ameloblastoma, though, is more aggressive and common, presenting a higher rate of recurrence than the unicystic and extraosseous/peripheral types; usually, the treatment of choice for this variant employs more invasive procedures. This report is of a 13-year-old male patient who presented with a swelling on the posterior mandibular region, on the left side of his face. Intraoral examination revealed lingual displacement of teeth 36 and 37. The clinical, radiographic and histopathological analyses confirmed the diagnosis of ameloblastoma (plexiform histological type). The patient was treated with curettage and peripheral osteotomy and a 5-year follow-up examination showed the area to have healed completely, with no evidence of recurrence. Consequently, although the clinical management of ameloblastoma is often based on invasive surgical approaches, large tumors treated with conservative surgery are less aesthetically and functionally impaired, and may demonstrate good prognosis with no recurrence in the 5-year follow-up period, as in the case reported here.
DOI: 10.6084/m9.figshare.11966025
title: Furosemide in water matrix: HPLC-UV method development and degradation studies

description: Abstract This study developed a method for furosemide quantification through high performance liquid chromatographic technique. Special attention was given to solute loss and storage stability due to furosemide’s low solubility and photosensitivity, respectively. The performance of Nylon and PVDF filters was tested in a 2 mg.L-1 furosemide solution. PVDF filters showed better recovery capacity and therefore are more suitable for furosemide filtration. Over eight days, three different storage conditions were studied to access furosemide degradation susceptibility: (i) exposure to light at room temperature, (ii) storage at room temperature without exposure to light, and (iii) storage at 4ºC without exposure to light. The study demonstrated that after 48 h under natural light exposure furosemide was completely degraded. Furosemide solution stored in the dark was stable. Storage temperature did not seem to affect furosemide concentration. The study shows that the selection of more suitable filter and storage conditions for furosemide determination is crucial to avoid underestimation errors.
DOI: 10.6084/m9.figshare.5644615.v1
title: The Bolsa Família Program and health conditionalities: challenges of intergovernmental and intersectoral coordination

description: ABSTRACT The existence of health conditionalities in income transfer programs is controversial and requires coordination between social areas. The aim of the study was to discuss the health conditionalities of the Bolsa Família Program, considering national guidelines and the intergovernmental and intersectoral relations. It involved literature review, documental analysis, analysis of secondary data and twenty interviews. Frailties have been observed in the relations between levels of government and sectors in the program management. There was no evidence that the existence and monitoring of health conditionalities per se contributes to the right to health and interruption of the cycle of poverty.
DOI: 10.6084/m9.figshare.11452533
title: Analysis of probiotic antimicrobial activity and its adherence to orthodontic brackets: in vitro study

description: Abstract Introduction The presence of a fixed orthodontic appliance makes cleaning difficult and enhances the accumulation of bacterial biofilm on dental surfaces. It is the desire of researchers worldwide. Objective To verify the bacteriotherapeutic action of dairy products containing or not probiotics under Streptococcus mutans (SM) (ATCC 25175) and S salivarius (SS) (ATCC 7073) pools, as well as their adhesion to the surface of orthodontic brackets. Material and method Pool of SM and SS ATCC strains was formed and plated on a petri dish containing brain heart infusion agar (BHI) culture medium. After formation of the medium a hole was made in the center of the plate followed by filling with 150 µL of the products to be tested, forming the following groups: GL - Bovine milk; GLP - Probiotic bovine milk; GLF - Fermented milk and GLFP - Probiotic fermented milk. Following the plates were incubated for 48h in an oven at 37°C. After that the halo formed between the product and the medium was measured with a millimeter ruler. Already in the membrane disc was formed biofilm with the same pool of strains, under membrane discs. After this the serial dilution containing the product was made according to the group: P1 (water); P2 (L); P3 (LP); P4 (LFP), followed by plating and total microorganism count. For the adhesiveness of dairy products, orthodontic brackets were submerged in each solution (GL, GLP, GLF and GLFP), which were incubated at 37°C/24h. Subsequently, each bracket was transferred to an ependorf containing sterile saline solution, which was subjected to serial dilutions, then incubated at 37°C/48h under microaerophilia for counting CFU/mL. For data analysis, the Levene, Shapiro-Wilk and Kruskal-Wallis tests were used. The adopted significance level was 5% (α=0.05). Result There was no inhibition halo formation between the products and the culture medium (p&lt;0.05); In the membrane disc no statistical differences were observed between the groups (p=0.679); The group treated with probiotic bovine milk and probiotic fermented milk showed adherence to orthodontic brackets (p = 0.056). Conclusion The findings of the present study allow us to conclude that, in in vitro studies, it was not possible to verify bacteriotherapy from dairy products containing or not probiotics in SM and SS strains.
DOI: 10.6084/m9.figshare.11452533.v1
title: Analysis of probiotic antimicrobial activity and its adherence to orthodontic brackets: in vitro study

description: Abstract Introduction The presence of a fixed orthodontic appliance makes cleaning difficult and enhances the accumulation of bacterial biofilm on dental surfaces. It is the desire of researchers worldwide. Objective To verify the bacteriotherapeutic action of dairy products containing or not probiotics under Streptococcus mutans (SM) (ATCC 25175) and S salivarius (SS) (ATCC 7073) pools, as well as their adhesion to the surface of orthodontic brackets. Material and method Pool of SM and SS ATCC strains was formed and plated on a petri dish containing brain heart infusion agar (BHI) culture medium. After formation of the medium a hole was made in the center of the plate followed by filling with 150 µL of the products to be tested, forming the following groups: GL - Bovine milk; GLP - Probiotic bovine milk; GLF - Fermented milk and GLFP - Probiotic fermented milk. Following the plates were incubated for 48h in an oven at 37°C. After that the halo formed between the product and the medium was measured with a millimeter ruler. Already in the membrane disc was formed biofilm with the same pool of strains, under membrane discs. After this the serial dilution containing the product was made according to the group: P1 (water); P2 (L); P3 (LP); P4 (LFP), followed by plating and total microorganism count. For the adhesiveness of dairy products, orthodontic brackets were submerged in each solution (GL, GLP, GLF and GLFP), which were incubated at 37°C/24h. Subsequently, each bracket was transferred to an ependorf containing sterile saline solution, which was subjected to serial dilutions, then incubated at 37°C/48h under microaerophilia for counting CFU/mL. For data analysis, the Levene, Shapiro-Wilk and Kruskal-Wallis tests were used. The adopted significance level was 5% (α=0.05). Result There was no inhibition halo formation between the products and the culture medium (p&lt;0.05); In the membrane disc no statistical differences were observed between the groups (p=0.679); The group treated with probiotic bovine milk and probiotic fermented milk showed adherence to orthodontic brackets (p = 0.056). Conclusion The findings of the present study allow us to conclude that, in in vitro studies, it was not possible to verify bacteriotherapy from dairy products containing or not probiotics in SM and SS strains.
DOI: 10.6084/m9.figshare.9870977.v1
title: Assessment of the effects of vegetational cover on the long-term stabilityof a waste rock dump

description: Abstract Waste rock dump reclamation includes re-grading the banks to flatten the overall slope followed by re-vegetation. These measures are required to reduce surface erosion, provide physical stability, and meet future use goals. In some cases, the presence of trees can cause localized shear zones in a waste rock dump by the load of the tree mass and the action of wind, the presence of roots in the soil and the death of some species. In geotechnical assessment, however, the influence of trees on slope stability of waste rock dumps is only qualitative. This article assesses the effects of trees on slope stability of a reclaimed waste rock dump of an iron ore mine. Historic data of construction, laboratory tests, and stability analysis were evaluated. Slope stability assessments were performed after an extensive geotechnical and forest campaign, considering two scenarios - the final waste rock dump without the vegetational cover and the final waste rock with vegetational cover. Tree vegetation in slopes produces both positive and negative effects on slope stability. For scenario 1, the lowest factor of safety occurs for a potential failure surface near the bottom of the waste dump, passing by a layer 6 m depth from the surface. In scenario 2 the factor of safety is increased by 10%. For deeper failure surface, the smallest factor of safety occurs 30 m deep. However, very tall trees at the top of the waste dump are subject to wind action, which has a negative impact on slope stability.
DOI: 10.6084/m9.figshare.7518539
title: Characterization of patients and procedures performed in the service to patients with special needs of Araraquara School of Dentistry – UNESP

description: AbstractObjectiveTo characterize the patients and dental procedures performed in Service to Patients with Special Needs of Araraquara School of Dentistry (FOAr) – UNESP.MethodologyFrom the dental records of patients treated at outpatient basis between 2007 and 2013, information regarding age at the beginning of treatment, type of disability, controlled medications used, number of dental sessions, and number and type of dental procedures performed was obtained.ResultOf the 232 records analyzed, more than half of the patients (56.0%) had mental retardation, cerebral palsy or congenital abnormalities caused by infectious agents associated with mental retardation. Among the congenital abnormalities, the most prevalent was the Down syndrome (9.9%). Only 20% of patients started treatment before the age of ten and 62.0% used anticonvulsants, antipsychotics, anxiolytics, antidepressants and antiepileptics. A total of 4,506 dental procedures were performed, 37.6% were preventive procedures and 62.4% curative procedures (restorative, 28.0%; periodontal, 13.9%; endodontic, 9.7%; surgical, 9.1%, and prosthetic 1.7%). Also was observed that for most patients (63%) up to 10 sessions were required for the completion of treatment.ConclusionMost patients with special needs who sought dental treatment at FOAr presented neurological involvement. Despite this service prioritize preventive procedures; most of the procedures carried out were curative, fact directly linked to late search for the dental treatment.
DOI: 10.6084/m9.figshare.7562756
title: Risk factors for new-onset diabetes mellitus after kidney transplantation (NODAT): a Brazilian single center study

description: ABSTRACT Objectives: This study aims to verify the new-onset diabetes after kidney transplant (NODAT) incidence in recipients within 1 year after kidney transplantation from a single center in Southern Brazil and to assess the associated conditions. Subjects and methods: A retrospective study of 258 post-renal transplant patients was performed. Demographic (gender, age, ethnic background) and clinical (origin of graft, associated infections, body mass index (BMI) at transplant time and 6 and 12 months after, causes of renal failure, and comorbidities) data were analyzed. All patients were on tacrolimus, mycophenolate mofetil, and prednisone treatment. Patients with and without NODAT were compared. Results: A NODAT incidence of 31.2% was noted 1 year post transplantation. In the univariate analysis, patients with NODAT were older (p = 0.001), mostly had African–American ethnic background (p = 0.02), and had renal failure secondary to high blood pressure (HBP) (p = 0.001). The group of patients with NODAT also had more incidence of post-transplant HBP (p = 0.01), heart failure (p = 0.02), and dyslipidemia (p = 0.001). Logistic regression showed that African–American ethnic background, post-transplant HBP, and dyslipidemia were independently associated with NODAT. Conclusion: This study shows a NODAT incidence that is greater in patients with African–American ethnic background and that is associated with HBP and dyslipidemia.
DOI: 10.6084/m9.figshare.7562756.v1
title: Risk factors for new-onset diabetes mellitus after kidney transplantation (NODAT): a Brazilian single center study

description: ABSTRACT Objectives: This study aims to verify the new-onset diabetes after kidney transplant (NODAT) incidence in recipients within 1 year after kidney transplantation from a single center in Southern Brazil and to assess the associated conditions. Subjects and methods: A retrospective study of 258 post-renal transplant patients was performed. Demographic (gender, age, ethnic background) and clinical (origin of graft, associated infections, body mass index (BMI) at transplant time and 6 and 12 months after, causes of renal failure, and comorbidities) data were analyzed. All patients were on tacrolimus, mycophenolate mofetil, and prednisone treatment. Patients with and without NODAT were compared. Results: A NODAT incidence of 31.2% was noted 1 year post transplantation. In the univariate analysis, patients with NODAT were older (p = 0.001), mostly had African–American ethnic background (p = 0.02), and had renal failure secondary to high blood pressure (HBP) (p = 0.001). The group of patients with NODAT also had more incidence of post-transplant HBP (p = 0.01), heart failure (p = 0.02), and dyslipidemia (p = 0.001). Logistic regression showed that African–American ethnic background, post-transplant HBP, and dyslipidemia were independently associated with NODAT. Conclusion: This study shows a NODAT incidence that is greater in patients with African–American ethnic background and that is associated with HBP and dyslipidemia.
DOI: 10.6084/m9.figshare.7514642
title: The Reliability of the Brief Visuospatial Memory Test - Revised in Brazilian multiple sclerosis patients

description: Abstract Cognitive Impairment (CI) is a common and distressing problem in Multiple Sclerosis (MS). Its identification is complicated and sometimes omitted in the routine evaluation by neurologists. The BICAMS (Brief International Cognitive Assessment for Multiple Sclerosis) is a promising tool to overcome this difficulty. However, there is some concern regarding the subjectivity in scoring of the BVMT-R (Brief Visuospatial Memory Test - Revised), one of its components. Objective: To evaluate the reliability of the BVMT-R in a sample of Brazilian MS patients, with the measure being administered and scored by neurologists. Methods: BICAMS was applied to seventy subjects comprising forty patients diagnosed with MS and thirty healthy controls. In the MS patients group, the coefficients of agreement between three different raters, using the same protocols, and the internal consistency of the BVMT-R were assessed. Also, the coefficients of correlation of the BVMT-R with the other tests of the BICAMS, CVLT II (California Verbal Learning Test II) and SDMT (Symbol Digit Modalities Test), and their respective effect sizes were calculated. Results: the BVMT-R presented a moderate inter-rater coefficient of agreement (k=0.62), an excellent Intraclass Correlation Coefficient (ICC=0.85), and high internal consistency (α=0.92). The correlation between the BVMT-R and CVLT II was moderate (ρ=0.36; p&lt;0.025), but strong with the SDMT (ρ=0.60; p&lt;0.01), with a large effect size. Conclusion: The BVMT-R is a reliable instrument for assessing CI in patients with MS, having a significant association with information processing speed, an aspect which should be considered when evaluating its score.
DOI: 10.6084/m9.figshare.7509323
title: LEARNING OF PROBOSCIS EXTENSION IN HARNESSED AFRICANIZED HONEY BEE DRONES (Apis mellifera L.)

description: Studies on the olfactive learning in honey bees (Apis mellifera L.) are predominantly performed with worker bees. In this study, we used the classical conditioning of proboscis extension (PER) to evaluate the effectiveness of 5 scents as conditioned stimuli (CS). Ten groups of 20 drones (A. mellifera L.) each were used. The conditioned stimuli were the odors of Citral, Hexanal, Geraniol, beeswax (comb), and beeswax (foundation sheet). In addition to the acquisition of learning, we measured the persistence of conditioning when the unconditioned stimulus was no longer presented (i.e.,extinction). The intertrial interval, the CS duration and US duration were 10 min, 2 sec, and 3 sec, respectively. The drones were able to demonstrate conditioning and storage of information. Citral, Hexanal, and beeswax (comb) were the most efficient stimuli in classical conditioning with drones.