cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7185602.v1
title: Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil

description: Abstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.
DOI: 10.7284/119105
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise TN322 using a Hawaii UHDAS instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185593
title: Influence of udder depth on cleaning teats and health of the mammary gland in robotic milking

description: ABSTRACT The aim of this study was to assess the influence of the depth of the udder on cleaning of teats and health of the mammary gland. The experiment was developed in the period spanning from March to June 2014, in Castro, PR. Twenty Holstein cows confined in free stall and milked in an automatic milking system (AMS) were used. The cows were divided into 4 groups with five animals (2 primiparous and 3 multiparous) based on the depth of the udder (udder depth: normal, small, and shallow). The electronically recorded data were collected monthly for four months, referring to the week of the official production milk control. To evaluate the effectiveness of cleaning teats, two contralateral teats were swabbed (right front quarter and left rear) before and after the automatic cleaning and once moistened towels were scrubbed around the other teats. A sample of milk was collected per cow to determine the somatic cell count (SCC). In the multivariate analysis relation of the udder depth with the teat cleaning was observed and evaluated through the teat cleaning with moistened towels score (TCTS). Udders slightly above the hocks (normal) had lower SCC and total bacterial count (TBC) of the teats. Shallow udders showed greater difference in TCTS. Cows with deep and shallow udders show less effective teat cleaning and health of the mammary gland. Cows with normal udders presented mammary gland conformation and health and contamination of teats most appropriate for robotic milking. On farms intending to introduce AMS it is recommended to select cows with udder depth which is a little above the hock. The impact of AMS on the TBC of the teats is related to the condition of cleaning of the teats pre-milking and housing conditions. Therefore, the condition of ambience of the cows is fundamental for the health of the mammary gland and obtaining high quality milk.
DOI: 10.6084/m9.figshare.7185593.v1
title: Influence of udder depth on cleaning teats and health of the mammary gland in robotic milking

description: ABSTRACT The aim of this study was to assess the influence of the depth of the udder on cleaning of teats and health of the mammary gland. The experiment was developed in the period spanning from March to June 2014, in Castro, PR. Twenty Holstein cows confined in free stall and milked in an automatic milking system (AMS) were used. The cows were divided into 4 groups with five animals (2 primiparous and 3 multiparous) based on the depth of the udder (udder depth: normal, small, and shallow). The electronically recorded data were collected monthly for four months, referring to the week of the official production milk control. To evaluate the effectiveness of cleaning teats, two contralateral teats were swabbed (right front quarter and left rear) before and after the automatic cleaning and once moistened towels were scrubbed around the other teats. A sample of milk was collected per cow to determine the somatic cell count (SCC). In the multivariate analysis relation of the udder depth with the teat cleaning was observed and evaluated through the teat cleaning with moistened towels score (TCTS). Udders slightly above the hocks (normal) had lower SCC and total bacterial count (TBC) of the teats. Shallow udders showed greater difference in TCTS. Cows with deep and shallow udders show less effective teat cleaning and health of the mammary gland. Cows with normal udders presented mammary gland conformation and health and contamination of teats most appropriate for robotic milking. On farms intending to introduce AMS it is recommended to select cows with udder depth which is a little above the hock. The impact of AMS on the TBC of the teats is related to the condition of cleaning of the teats pre-milking and housing conditions. Therefore, the condition of ambience of the cows is fundamental for the health of the mammary gland and obtaining high quality milk.
DOI: 10.7284/121088
title: Anemometer data collected during research cruise TN322 using a RM Young 06206 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/119107
title: CTD (Conductivity, Temperature, Depth) data collected during research cruise TN322 using a Sea-Bird SBE-911+ instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185581
title: Software for systematization of nursing care in medical units

description: ABSTRACT Objective: To describe the development of a software prototype to apply the nursing process in clinical units of a general hospital, and assess its usefulness. Method: Applied methodological research of technological production of a program based on prototyping software engineering developed in three stages: specification, development, and validation. Results: Produced under the name of INFOSAE, the system represents a set of stages (history, diagnosis, expected outcomes, and nursing intervention) providing guidance to nurses to direct and guarantee the necessary care to patients, also allowing an assessment of this care. Conclusion: The INFOSAE software prototype, an easy-to-use computerized technology, obtained a favorable assessment by the user nurses that participated in the whole development process, from the specification to the validation of the system.
DOI: 10.6084/m9.figshare.7185581.v1
title: Software for systematization of nursing care in medical units

description: ABSTRACT Objective: To describe the development of a software prototype to apply the nursing process in clinical units of a general hospital, and assess its usefulness. Method: Applied methodological research of technological production of a program based on prototyping software engineering developed in three stages: specification, development, and validation. Results: Produced under the name of INFOSAE, the system represents a set of stages (history, diagnosis, expected outcomes, and nursing intervention) providing guidance to nurses to direct and guarantee the necessary care to patients, also allowing an assessment of this care. Conclusion: The INFOSAE software prototype, an easy-to-use computerized technology, obtained a favorable assessment by the user nurses that participated in the whole development process, from the specification to the validation of the system.
DOI: 10.6084/m9.figshare.7185584
title: COMPARATIVE STUDY FOR ADSORPTION OF HYDROGEN-METHANE MIXTURES ON ACTIVATED CARBON AND 5A MOLECULAR SIEVE

description: Abstract Equilibrium adsorption isotherms were obtained experimentally by a static method for a binary component hydrogen-methane mixture on activated carbon and 5A molecular sieve adsorbents. The temperature range 303-333 K and pressure range 0 - 4 MPa were examined. Methods for predicting gas-mixture equilibrium adsorption data from the pure-component isotherms were evaluated. Extended Langmuir model correlated the adsorption of binary mixtures fairly well. In all cases mutual interference was found, that is, the amount of a given gas adsorbed at a given partial pressure was always less in the mixture than it would have been if the other gaseous component had not been present. It was found that the amount adsorbed of gases per unit bulk volume for 5A molecular sieve was higher than for activated carbon. Furthermore, 5A molecular sieve showed better adsorption characteristics than activated carbon in mass transfer properties, affinity, selectivity and heat of adsorption.
DOI: 10.7284/119108
title: GNSS (Global Navigation Satellite System) data collected during research cruise TN322 using a C&C C-Nav 3050 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185584.v1
title: COMPARATIVE STUDY FOR ADSORPTION OF HYDROGEN-METHANE MIXTURES ON ACTIVATED CARBON AND 5A MOLECULAR SIEVE

description: Abstract Equilibrium adsorption isotherms were obtained experimentally by a static method for a binary component hydrogen-methane mixture on activated carbon and 5A molecular sieve adsorbents. The temperature range 303-333 K and pressure range 0 - 4 MPa were examined. Methods for predicting gas-mixture equilibrium adsorption data from the pure-component isotherms were evaluated. Extended Langmuir model correlated the adsorption of binary mixtures fairly well. In all cases mutual interference was found, that is, the amount of a given gas adsorbed at a given partial pressure was always less in the mixture than it would have been if the other gaseous component had not been present. It was found that the amount adsorbed of gases per unit bulk volume for 5A molecular sieve was higher than for activated carbon. Furthermore, 5A molecular sieve showed better adsorption characteristics than activated carbon in mass transfer properties, affinity, selectivity and heat of adsorption.
DOI: 10.7284/121258
title: GNSS (Global Navigation Satellite System) data collected during research cruise TN322 using a C&C C-Nav 2000 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185566
title: Electrical Immunosensor Made with Antigenic Peptide NS5A-1 Immobilized onto Silk Fibroin for Diagnosing Hepatitis C

description: Immunosensors based on impedance spectroscopy for diagnosing hepatitis C are reported where the sensing units were made with the antigenic peptide PPLLESWKDPDYVPPWHG (NS5A-1) derived from the NS5A protein of the hepatitis C virus (HCV) immobilized in layer-by-layer (LbL) films with silk fibroin (SF) and deposited on gold interdigitated electrodes. The electrical response of the sensing units varied upon immersion into solutions containing the antibody anti-HCV owing to the biomolecular recognition of NS5A-1. This was associated with morphological changes on the LbL films caused by adsorption of NS5A-1 and inferred from atomic force microscopy images. Buffer solutions with different anti-HCV concentrations down to 2 ng mL-1 could be clearly distinguished by analyzing the impedance spectroscopy data with a multidimensional projection technique. The specificity toward anti-HCV antibodies was confirmed in control experiments where no significant changes in the electrical response were measured by exposing the sensing units to solutions containing an anti-human immunodeficiency virus (HIV) antibody. The high sensitivity and selectivity of the units made with LbL films demonstrate the feasibility of measuring electrical impedance as an immunosensing strategy to detect hepatitis C.
DOI: 10.6084/m9.figshare.7185563
title: Cultivar affects the color change kinetics of sugarcane juice

description: Abstract This study evaluated the influence of different Brazilian cultivars (RB867515/C1, SP813250/C2, RB92579/C3 and CTC02/C4) on unprocessed sugarcane juice quality by physicochemical, microbiological and enzymatic analysis. Color parameters were determined during six days at 10 °C. The experimental design was completely randomized with four treatments (cultivars) and three replicates. Significant differences were observed (p ≤ 0.05) among the juice samples from different cultivars. The pH values ranged from 5.09 to 5.25, soluble solids content from 14.13 to 18.60 ºBrix and titratable acidity from 0.035 to 0.120% of citric acid. Counts of mesophiles, psychrotrophs and molds and yeasts varied between 4.8 and 6.2 logCFU mL-1, 3.2 and 4.6 logCFU mL-1 and 3.3 and 4.8 logCFU mL-1 , respectively. Polyphenol oxidase and peroxidase activities ranged from 8 to 15 U mL -1 and between 6 and 40 U mL-1, respectively. The initial (t0) lightness varied from 21 to 37 among cultivars, and final values, after six days of storage (t6), between 22 and 41. Principal components analysis showed that the juice of different cultivars is characterized by different parameters. Considering its lighter color and smaller color variation during storage, juice extracted from cultivar RB867515 presented the greatest potential for consumer acceptance.
DOI: 10.6084/m9.figshare.7185566.v1
title: Electrical Immunosensor Made with Antigenic Peptide NS5A-1 Immobilized onto Silk Fibroin for Diagnosing Hepatitis C

description: Immunosensors based on impedance spectroscopy for diagnosing hepatitis C are reported where the sensing units were made with the antigenic peptide PPLLESWKDPDYVPPWHG (NS5A-1) derived from the NS5A protein of the hepatitis C virus (HCV) immobilized in layer-by-layer (LbL) films with silk fibroin (SF) and deposited on gold interdigitated electrodes. The electrical response of the sensing units varied upon immersion into solutions containing the antibody anti-HCV owing to the biomolecular recognition of NS5A-1. This was associated with morphological changes on the LbL films caused by adsorption of NS5A-1 and inferred from atomic force microscopy images. Buffer solutions with different anti-HCV concentrations down to 2 ng mL-1 could be clearly distinguished by analyzing the impedance spectroscopy data with a multidimensional projection technique. The specificity toward anti-HCV antibodies was confirmed in control experiments where no significant changes in the electrical response were measured by exposing the sensing units to solutions containing an anti-human immunodeficiency virus (HIV) antibody. The high sensitivity and selectivity of the units made with LbL films demonstrate the feasibility of measuring electrical impedance as an immunosensing strategy to detect hepatitis C.
DOI: 10.7284/121259
title: Gyrocompass data collected during research cruise TN322 using a Sperry MK-37 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185563.v1
title: Cultivar affects the color change kinetics of sugarcane juice

description: Abstract This study evaluated the influence of different Brazilian cultivars (RB867515/C1, SP813250/C2, RB92579/C3 and CTC02/C4) on unprocessed sugarcane juice quality by physicochemical, microbiological and enzymatic analysis. Color parameters were determined during six days at 10 °C. The experimental design was completely randomized with four treatments (cultivars) and three replicates. Significant differences were observed (p ≤ 0.05) among the juice samples from different cultivars. The pH values ranged from 5.09 to 5.25, soluble solids content from 14.13 to 18.60 ºBrix and titratable acidity from 0.035 to 0.120% of citric acid. Counts of mesophiles, psychrotrophs and molds and yeasts varied between 4.8 and 6.2 logCFU mL-1, 3.2 and 4.6 logCFU mL-1 and 3.3 and 4.8 logCFU mL-1 , respectively. Polyphenol oxidase and peroxidase activities ranged from 8 to 15 U mL -1 and between 6 and 40 U mL-1, respectively. The initial (t0) lightness varied from 21 to 37 among cultivars, and final values, after six days of storage (t6), between 22 and 41. Principal components analysis showed that the juice of different cultivars is characterized by different parameters. Considering its lighter color and smaller color variation during storage, juice extracted from cultivar RB867515 presented the greatest potential for consumer acceptance.
DOI: 10.6084/m9.figshare.7185557
title: Chemical composition, percent of dietary reference intake, and acceptability of gluten-free bread made from Prosopis nigra flour, added with hydrocolloids

description: Abstract The objective of the work was to know the nutritional contribution of gluten-free bread made with Prosopis nigra flour, and the acceptability measured by celiac and non celiac persons. Gluten-free bread was made from a mixture of corn, rice and Prosopis nigra flours. To achieve better technological characteristics, xanthan gum and whey concentrate were added. A portion of bread (50g) provided 19% of DRI for protein for children aged 4 to 8 years, while for a pregnant or lactating woman, this value reached 5%. Regarding the fibers, 50g of bread covered 16% and 14% of the DRI for these biological moments. The fat requirement was covered by 16% for all biological moments analyzed. The dietary contribution of this bread surpasses in proteins and fiber to the commercial ones. Bread made with Prosopis nigra flour presented a balanced caloric distribution, contributing a high proportion of proteins, fats, dietary fiber (mainly of the insoluble fraction), and low quantity of carbohydrates available, characteristics that make it a recommendable product for celiac persons in different biological moments.
DOI: 10.6084/m9.figshare.7185557.v1
title: Chemical composition, percent of dietary reference intake, and acceptability of gluten-free bread made from Prosopis nigra flour, added with hydrocolloids

description: Abstract The objective of the work was to know the nutritional contribution of gluten-free bread made with Prosopis nigra flour, and the acceptability measured by celiac and non celiac persons. Gluten-free bread was made from a mixture of corn, rice and Prosopis nigra flours. To achieve better technological characteristics, xanthan gum and whey concentrate were added. A portion of bread (50g) provided 19% of DRI for protein for children aged 4 to 8 years, while for a pregnant or lactating woman, this value reached 5%. Regarding the fibers, 50g of bread covered 16% and 14% of the DRI for these biological moments. The fat requirement was covered by 16% for all biological moments analyzed. The dietary contribution of this bread surpasses in proteins and fiber to the commercial ones. Bread made with Prosopis nigra flour presented a balanced caloric distribution, contributing a high proportion of proteins, fats, dietary fiber (mainly of the insoluble fraction), and low quantity of carbohydrates available, characteristics that make it a recommendable product for celiac persons in different biological moments.
DOI: 10.7284/121087
title: Meteorological Station data collected during research cruise TN322 using a RM Young 26800 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185554
title: Extraction of matrine from Sophora flavescens Ait. and evaluation of its inhibitory effects on human nasopharyngeal carcinoma CNE-2 cells

description: Abstract The present study investigated the extraction and purification technology of matrine from Sophora flavescens Ait. and the inhibitory effects of matrine on human nasopharyngeal carcinoma (NPC) CNE-2 cells. The matrine was extracted from Sophora flavescens Ait. by decocting using ethanol-aqueous solution as the solvent, followed by purification using ion exchange resin adsorption. The extraction and purification conditions were investigated. The effects of matrine on growth, cell apoptosis and cycle of CNE-2 cells and expressions of Bac-2 and caspase-3 protein in cells were detected. Results showed that, the optimal extraction and purification technologies of matrine from Sophora flavescens Ait. were obtained, under which the purity of matrine was 81.56%. Matrine could obtain inhibit the growth of CNE-2 cells, promote the cell apoptosis, and arrest most cells in G0/G1 phase. In addition, after treated matrine, the relative expression level of Bcl-2 protein in CNE-2 cells was decreased, and the expression level of Caspase-3 protein was increased. In conclusion, matrine has inhibitory effects on human NPC CNE-2 cells. The mechanism may be related to its down-regulation of Bcl-2 protein and up-regulation of Caspase-3 protein expression.
DOI: 10.6084/m9.figshare.7185554.v1
title: Extraction of matrine from Sophora flavescens Ait. and evaluation of its inhibitory effects on human nasopharyngeal carcinoma CNE-2 cells

description: Abstract The present study investigated the extraction and purification technology of matrine from Sophora flavescens Ait. and the inhibitory effects of matrine on human nasopharyngeal carcinoma (NPC) CNE-2 cells. The matrine was extracted from Sophora flavescens Ait. by decocting using ethanol-aqueous solution as the solvent, followed by purification using ion exchange resin adsorption. The extraction and purification conditions were investigated. The effects of matrine on growth, cell apoptosis and cycle of CNE-2 cells and expressions of Bac-2 and caspase-3 protein in cells were detected. Results showed that, the optimal extraction and purification technologies of matrine from Sophora flavescens Ait. were obtained, under which the purity of matrine was 81.56%. Matrine could obtain inhibit the growth of CNE-2 cells, promote the cell apoptosis, and arrest most cells in G0/G1 phase. In addition, after treated matrine, the relative expression level of Bcl-2 protein in CNE-2 cells was decreased, and the expression level of Caspase-3 protein was increased. In conclusion, matrine has inhibitory effects on human NPC CNE-2 cells. The mechanism may be related to its down-regulation of Bcl-2 protein and up-regulation of Caspase-3 protein expression.
DOI: 10.7284/121090
title: Radiometer data collected during research cruise TN322 using a Eppley RAD instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition