cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5123926
title: Supplementary Material for: Molecular Characterization of Xp Chromosome Deletion in a Fertile Cow

description: A young cow of the Marchigiana breed (central Italy) with normal body conformation and external genitalia underwent routine cytogenetic analyses prior to its use for reproduction. After normal chromosome staining, only one X chromosome was observed with a normal diploid number (2n = 60) in all 200 studied cells. Subsequent cytogenetic analyses by using both CBA- and RBA-banding techniques evidenced that almost all the p arms of the other X chromosome was lacking. Detailed FISH-mapping analyses with BAC covering this Xp arm region demonstrated that this large chromosome region was deleted. RBA-banding showed that the deleted X was late replicating. CGH array analysis evidenced that deletion involves the Xp arm from the telomere to around 39.5 Mb, referring to the BosTau6 cattle genome assembly. This abnormality deletes about 40 Mb of the X chromosome sequence, but, despite the large number of genes deleted, none of them are programmed to escape from inactivation. This can explain the normal phenotype of the female which is actually pregnant. Finally, we evidenced, by analysis of an SNP mapped to the deleted region (SNP rs29024121), that the only normal (e.g. nondeleted) X chromosome present derives from the father. Hence, the deletion has a maternal origin.
DOI: 10.6084/m9.figshare.5123923
title: Supplementary Material for: Acutely Ill Patients in Internal Medicine Departments Want Treatment for Undiagnosed, Symptomatic Skin Conditions

description: <b><i>Objective:</i></b> Concomitant skin conditions may be neglected in internal medicine patients due to lack of knowledge or resources. Thus, we investigated the prevalence of undiagnosed skin conditions in this population. <b><i>Methods:</i></b> 200 patients in a university medical center’s internal medicine division were examined clinically for dermatoses and quality of life in a prospective, 2-month, single-center study. <b><i>Results:</i></b> All patients had several dermatological problems (mean per patient: 13; range: 3–25). There was no relationship between the patient’s main medical problem and the number or nature of dermatological conditions. Most patients (84%) requested treatment for their skin condition during hospitalization, especially for xerosis (76%), warts (69%), seborrheic eczema (67%) and onychorrhexis (53%) but not for asymptomatic dermatoses. The impairment in skin-related quality of life was mild but significant, with a mean ± SD Dermatology Life Quality Index of 3 ± 4 (p &lt; 0.001), and global quality of life impairment was severe (p &lt; 0.001). <b><i>Conclusions:</i></b> Inpatients suffered from many different, mostly age-related, skin conditions that remained undiagnosed. When prompted, however, patients requested treatment, particularly for symptomatic dermatological conditions such as xerosis, revealing an unmet need that needs to be addressed by qualified evaluation and care.
DOI: 10.6084/m9.figshare.5123929.v1
title: Supplementary Material for: Decline in Gait Performance Detected by an Electronic Walkway System in 907 Older Adults of the Population-Based KORA-Age Study

description: <b><i>Background:</i></b> Gait changes at older ages are a strong predictor of a decline in lower extremity functions. However, large population-based studies assessing gait parameters in various gait tasks are lacking. <b><i>Objective:</i></b> We investigated the relationship of age, the use of mobility aids and being fitted with an endoprosthesis with selected gait parameters, assessed in different walking tasks. <b><i>Methods:</i></b> In the population-based KORA-Age study, data from 907 men and women aged 65-91 years were obtained using the validated electronic walkway system GAITRite, which quantifies spatiotemporal gait parameters in the measurement range of a 488 × 61 cm walkway mat. Participants completed three walking tasks at different speeds (normal, slow and fast) and a fourth walking task at normal speed with the additional task of counting backwards (dual-task walking). Additionally, the impact of endoprostheses (hip or knee) and mobility aids was assessed. <b><i>Results:</i></b> The highest relative age-related decline for velocity was observed during dual-task walking (26.1% for men and 23.4% for women) and for step length during fast walking (20.2 and 14.4%) when comparing participants aged &lt;70 years with those aged ≥85 years. Weaker performances for velocity, cadence and step length were observed among women with knee or hip endoprostheses (fast walking speed) (p &lt; 0.05). Across all walking tasks, significant differences between mobility aid users and nonusers were observed for velocity and step length among both men and women (p &lt; 0.05). <b><i>Conclusion:</i></b> A decline in gait performance is most notable in fast speed and dual-task walking, in age-related endoprosthesis and mobility aid analyses. The marked relative decrease in gait parameters in these difficult gait tasks may be attributed to lacking resources for compensation among the elderly.
DOI: 10.6084/m9.figshare.5123914
title: Supplementary Material for: Association between Physical Activity and Risk of Stroke Subtypes: The Atherosclerosis Risk in Communities Study

description: <b><i>Background:</i></b> The relationship between stroke subtypes and physical activity is unclear. <b><i>Methods:</i></b> Using data from 13,069 men and women aged 45–64 years who participated in the Atherosclerosis Risk in Communities Study, physical activity was assessed by self-report using the Baecke questionnaire at baseline (1987–1989). The American Heart Association’s ideal cardiovascular health guidelines served as a basis for the calculation of three physical activity categories: poor, intermediate, and ideal. Stroke and its subtypes were ascertained from physician review of medical records. Multivariable adjusted hazard ratios (HR) and 95% confidence intervals (CI) were calculated using Cox regression models. <b><i>Results:</i></b> During a median follow-up of 18.8 years, a total of 648 incident ischemic strokes occurred. Significant inverse associations were found between physical activity categories and total, total ischemic, and nonlacunar stroke in adjusted models (age, sex, race-center, education, cigarette-years). Compared with poor physical activity, the adjusted HR (95% CI) for ideal physical activity were 0.78 (0.62–0.97) for total, 0.76 (0.59–0.96) for total ischemic, 0.85 (0.51–1.40) for lacunar, 0.77 (0.47–1.27) for cardioembolic, and 0.71 (0.51–0.99) for nonlacunar stroke. Additional adjustments for waist-to-hip ratio, systolic blood pressure, antihypertensive medication, diabetes, left ventricular hypertrophy and laboratory parameters attenuated the HR. Further sex- and race-specific analyses revealed that the association was predominantly observed among males and among African-Americans. <b><i>Conclusion:</i></b> These data suggest a tendency toward a reduced risk of total, total ischemic, and nonlacunar stroke with higher levels of physical activity.
DOI: 10.6084/m9.figshare.5123917
title: Supplementary Material for: Neuroimmunomodulatory Alterations in Non-Lesional Peritoneum Close to Peritoneal Endometriosis

description: <b><i>Objectives:</i></b> An imbalance in the ratio of sensory to sympathetic nerve fibre (NF) density in peritoneal endometriotic lesions (pEL) has recently been demonstrated and leads to the assumption that this preponderance of the sensory pro-inflammatory milieu is a major cause of pain in endometriosis. Therefore, the density of sensory and sympathetic NFs was determined in distal unaffected peritoneum of endometriosis patients to be able to detect possible alterations in unaffected peritoneum. <b><i>Methods:</i></b> In serial pEL sections (n = 40), lesional and matching unaffected peritoneum as well as healthy peritoneum (HP) from patients without endometriosis (n = 15) were immunohistochemically analysed to identify protein gene product 9.5-, substance P- and tyrosine hydroxylase-positive NFs (intact, sensory and sympathetic NFs, respectively). In addition, the amount of immune cell infiltrates and the expression of nerve growth factor (NGF) and interleukin (IL)-1β in nerves of peritoneal endometriotic specimens were compared to those in the HP. <b><i>Results:</i></b> The overall NF density in the non-lesional, unaffected peritoneum of endometriosis patients is significantly reduced in comparison to both HP and pEL, while sensory NFs remain the same; the sympathetic NF density is significantly decreased compared to HP, but is still higher than the density close to the pEL. Immune cell infiltrates as well as NGF and IL-1β expression in nerves is significantly elevated in distal unaffected peritoneum in comparison to HP. <b><i>Conclusion:</i></b> The altered NF density in the non-lesional, unaffected peritoneum of endometriosis patients suggests new aspects in the understanding of the development of endometriosis and pain management in endometriosis.
DOI: 10.6084/m9.figshare.5123920
title: Supplementary Material for: Figur- und gewichtsbezogene Sorgen bei Frauen im mittleren Lebensalter und der Zusammenhang mit negativer Affektivität und Bildung

description: <b><i>Hintergrund:</i></b> Der Zusammenhang zwischen negativer Affektivität, Bildung und den figur- und gewichtsbezogenen Sorgen ist für junge Frauen weitgehend erforscht. Hingegen gibt es kaum Studien, welche die entsprechenden Faktoren bei Frauen im mittleren Lebensalter untersuchen. Ziel dieser Studie war es zu prüfen, ob es einen Zusammenhang zwischen Depressivität, Ängstlichkeit, zwanghaftem Verhalten und den figur- und gewichtsbezogenen Sorgen bei Frauen im mittleren Lebensalter gibt. Zudem wurde die Rolle des Bildungsniveaus untersucht. <b><i>Methoden:</i></b> Im Rahmen einer Online-Untersuchung zum Essverhalten bei Frauen über 40 Jahren wurden, neben sozioökonomischen Variablen, die figur- und gewichtsbezogenen Sorgen der Teilnehmerinnen mittels einer validierten deutschen Version des «Eating Disorder Examination Questionnaire» (EDE-Q) erfasst. Zusätzlich wurde eine deutsche Version des «Brief Symptom Inventory» (BSI) eingesetzt. In die Datenanalyse wurden die Ergebnisse von 550 Frauen im Alter von 40 bis 65 Jahren einbezogen. <b><i>Ergebnisse: </i></b>Depressivität, Ängstlichkeit und Zwanghaftigkeit korrelieren positiv mit figur- und gewichtsbezogenen Sorgen. Bei Frauen mit einem niedrigeren Bildungsniveau war der Zusammenhang zwischen Depressivität und figur- und gewichtsbezogenen Sorgen höher als bei Frauen mit einem höheren Bildungsniveau. Berechnungen anhand linearer Regressionsanalysen zeigten, dass Depressivität und Zwanghaftigkeit die stärksten Prädiktoren für figur- und essensbezogene Sorgen für beide Bildungsgruppen waren. <b><i>Diskussion:</i></b> Wenngleich die Ergebnisse dieser Studie bei gesunden Frauen im mittleren Lebensalter erhoben wurden, gibt der beobachtete Zusammenhang zwischen negativer Affektivität, figur- und gewichtsbezogenen Sorgen und Bildung Hinweise auf eine Frühindikation bzw. Prävention von Essstörungen.
DOI: 10.6084/m9.figshare.5123926.v1
title: Supplementary Material for: Molecular Characterization of Xp Chromosome Deletion in a Fertile Cow

description: A young cow of the Marchigiana breed (central Italy) with normal body conformation and external genitalia underwent routine cytogenetic analyses prior to its use for reproduction. After normal chromosome staining, only one X chromosome was observed with a normal diploid number (2n = 60) in all 200 studied cells. Subsequent cytogenetic analyses by using both CBA- and RBA-banding techniques evidenced that almost all the p arms of the other X chromosome was lacking. Detailed FISH-mapping analyses with BAC covering this Xp arm region demonstrated that this large chromosome region was deleted. RBA-banding showed that the deleted X was late replicating. CGH array analysis evidenced that deletion involves the Xp arm from the telomere to around 39.5 Mb, referring to the BosTau6 cattle genome assembly. This abnormality deletes about 40 Mb of the X chromosome sequence, but, despite the large number of genes deleted, none of them are programmed to escape from inactivation. This can explain the normal phenotype of the female which is actually pregnant. Finally, we evidenced, by analysis of an SNP mapped to the deleted region (SNP rs29024121), that the only normal (e.g. nondeleted) X chromosome present derives from the father. Hence, the deletion has a maternal origin.
DOI: 10.6084/m9.figshare.5123911
title: Supplementary Material for: Voice Risk Factors in Kindergarten Teachers in Greece

description: <b><i>Objective:</i></b> The purpose of this study was to identify voice risk factors for female kindergarten teachers and nurses in Heraklion, Crete. <b><i>Patients and Methods:</i></b> A questionnaire consisting of voice use and lifestyle activities was given to 200 kindergarten teachers in Heraklion, Crete, of which 151 were returned (75.5%). A group of 89 nurses served as the control group. Both groups also completed the Greek version of the Voice Handicap Index (VHI-G). <b><i>Results:</i></b> Kindergarten teachers sing more often, speak loudly more often when they are at work and present with more infections of the upper respiratory tract compared to nurses. They talk less than 30 min per day on the phone, drink less alcohol and water and smoke less in comparison with nurses. The median VHI-G score for the kindergarten teachers was significantly higher than that for the nurses. <b><i>Conclusion:</i></b> The results suggest that there is a difference in the factors that may be responsible for the appearance of voice problems in kindergarten teachers and in nurses. For kindergarten teachers, the risk factors for voice disorders are primarily related to vocal load factors and for nurses appear to be lifestyle-related more than voice use alone.
DOI: 10.6084/m9.figshare.5123917.v1
title: Supplementary Material for: Neuroimmunomodulatory Alterations in Non-Lesional Peritoneum Close to Peritoneal Endometriosis

description: <b><i>Objectives:</i></b> An imbalance in the ratio of sensory to sympathetic nerve fibre (NF) density in peritoneal endometriotic lesions (pEL) has recently been demonstrated and leads to the assumption that this preponderance of the sensory pro-inflammatory milieu is a major cause of pain in endometriosis. Therefore, the density of sensory and sympathetic NFs was determined in distal unaffected peritoneum of endometriosis patients to be able to detect possible alterations in unaffected peritoneum. <b><i>Methods:</i></b> In serial pEL sections (n = 40), lesional and matching unaffected peritoneum as well as healthy peritoneum (HP) from patients without endometriosis (n = 15) were immunohistochemically analysed to identify protein gene product 9.5-, substance P- and tyrosine hydroxylase-positive NFs (intact, sensory and sympathetic NFs, respectively). In addition, the amount of immune cell infiltrates and the expression of nerve growth factor (NGF) and interleukin (IL)-1β in nerves of peritoneal endometriotic specimens were compared to those in the HP. <b><i>Results:</i></b> The overall NF density in the non-lesional, unaffected peritoneum of endometriosis patients is significantly reduced in comparison to both HP and pEL, while sensory NFs remain the same; the sympathetic NF density is significantly decreased compared to HP, but is still higher than the density close to the pEL. Immune cell infiltrates as well as NGF and IL-1β expression in nerves is significantly elevated in distal unaffected peritoneum in comparison to HP. <b><i>Conclusion:</i></b> The altered NF density in the non-lesional, unaffected peritoneum of endometriosis patients suggests new aspects in the understanding of the development of endometriosis and pain management in endometriosis.
DOI: 10.6084/m9.figshare.5123905
title: Supplementary Material for: Methylated DNA and Total DNA in Serum Detected by One-Step Methylation-Specific PCR Is Predictive of Poor Prognosis for Breast Cancer Patients

description: <b><i>Purpose:</i></b> We recently developed the one-step methylation-specific PCR (OS-MSP) assay which can detect methylated DNA (met-DNA) in serum with high sensitivity. To examine its prognostic value, we applied this new assay to the detection of met-DNA in serum of breast cancer patients. <b><i>Methods:</i></b> Serum samples taken before surgery from 336 primary invasive breast cancer patients were subjected to the OS-MSP assay for the promoter regions of <i>GSTP1</i>,<i> RASSF1A</i>, and <i>RAR</i>β<i>2</i>. The assay outcome was considered positive when methylation was detected in at least one of these three genes. Total DNA content in serum was also determined. <b><i>Results:</i></b> Of the 336 stage I/II patients, 33 (10%) were positive for met-DNA in serum and showed a significantly worse overall survival (OS) rate at 100 months (78 vs. 95%; p = 0.002) than those with negative findings (n = 303). Patients with high total DNA in serum (n = 112) also showed a significantly worse OS rate at 100 months (86 vs. 97%; p = 0.001) than those with low total DNA in serum (n = 224). Moreover, patients both positive for met-DNA and with high total DNA in serum (n = 18) showed a much worse OS rate at 100 months (65 vs. 94%; p &lt; 0.001) than the others (n = 318). <b><i>Conclusions:</i></b> Met-DNA in serum detected with the OS-MSP assay constitutes a significant and independent prognostic factor, and its combination with total DNA in serum seems to be even more effective for prediction of prognosis for breast cancer patients.
DOI: 10.6084/m9.figshare.5123908
title: Supplementary Material for: Tissue Plasminogen Activator-Assisted Vitrectomy for Ruptured Eye with Suprachoroidal Hemorrhage

description: <b><i>Purpose:</i></b> To report a case of a ruptured eye with a suprachoroidal hemorrhage (SCH) in which tissue plasminogen activator (tPA)-assisted vitrectomy was successful in reconstructing the globe and restoring good vision. <b><i>Case:</i></b> A 32-year-old man was struck on the right eye by a surfboard. His eye was ruptured and his visual acuity decreased to hand movements. Surgery was immediately performed to successfully close the ruptured globe. Nine days later, a second surgery was performed, and tPA (25 µg/0.1 ml monteplase) was used to liquefy and drain the SCH. This freed enough vitreous space for a more comprehensive vitrectomy. Eighteen months after the injury, the retina remained attached, and the decimal best-corrected visual acuity improved to 0.8. <b><i>Conclusion:</i></b> tPA was helpful in lysing a massive SCH, thereby contributing to the excellent visual outcome. tPA-assisted drainage should be considered in cases of massive SCH when drainage is difficult due to an incomplete lysis of the clot.
DOI: 10.6084/m9.figshare.5123914.v1
title: Supplementary Material for: Association between Physical Activity and Risk of Stroke Subtypes: The Atherosclerosis Risk in Communities Study

description: <b><i>Background:</i></b> The relationship between stroke subtypes and physical activity is unclear. <b><i>Methods:</i></b> Using data from 13,069 men and women aged 45–64 years who participated in the Atherosclerosis Risk in Communities Study, physical activity was assessed by self-report using the Baecke questionnaire at baseline (1987–1989). The American Heart Association’s ideal cardiovascular health guidelines served as a basis for the calculation of three physical activity categories: poor, intermediate, and ideal. Stroke and its subtypes were ascertained from physician review of medical records. Multivariable adjusted hazard ratios (HR) and 95% confidence intervals (CI) were calculated using Cox regression models. <b><i>Results:</i></b> During a median follow-up of 18.8 years, a total of 648 incident ischemic strokes occurred. Significant inverse associations were found between physical activity categories and total, total ischemic, and nonlacunar stroke in adjusted models (age, sex, race-center, education, cigarette-years). Compared with poor physical activity, the adjusted HR (95% CI) for ideal physical activity were 0.78 (0.62–0.97) for total, 0.76 (0.59–0.96) for total ischemic, 0.85 (0.51–1.40) for lacunar, 0.77 (0.47–1.27) for cardioembolic, and 0.71 (0.51–0.99) for nonlacunar stroke. Additional adjustments for waist-to-hip ratio, systolic blood pressure, antihypertensive medication, diabetes, left ventricular hypertrophy and laboratory parameters attenuated the HR. Further sex- and race-specific analyses revealed that the association was predominantly observed among males and among African-Americans. <b><i>Conclusion:</i></b> These data suggest a tendency toward a reduced risk of total, total ischemic, and nonlacunar stroke with higher levels of physical activity.
DOI: 10.6084/m9.figshare.5123920.v1
title: Supplementary Material for: Figur- und gewichtsbezogene Sorgen bei Frauen im mittleren Lebensalter und der Zusammenhang mit negativer Affektivität und Bildung

description: <b><i>Hintergrund:</i></b> Der Zusammenhang zwischen negativer Affektivität, Bildung und den figur- und gewichtsbezogenen Sorgen ist für junge Frauen weitgehend erforscht. Hingegen gibt es kaum Studien, welche die entsprechenden Faktoren bei Frauen im mittleren Lebensalter untersuchen. Ziel dieser Studie war es zu prüfen, ob es einen Zusammenhang zwischen Depressivität, Ängstlichkeit, zwanghaftem Verhalten und den figur- und gewichtsbezogenen Sorgen bei Frauen im mittleren Lebensalter gibt. Zudem wurde die Rolle des Bildungsniveaus untersucht. <b><i>Methoden:</i></b> Im Rahmen einer Online-Untersuchung zum Essverhalten bei Frauen über 40 Jahren wurden, neben sozioökonomischen Variablen, die figur- und gewichtsbezogenen Sorgen der Teilnehmerinnen mittels einer validierten deutschen Version des «Eating Disorder Examination Questionnaire» (EDE-Q) erfasst. Zusätzlich wurde eine deutsche Version des «Brief Symptom Inventory» (BSI) eingesetzt. In die Datenanalyse wurden die Ergebnisse von 550 Frauen im Alter von 40 bis 65 Jahren einbezogen. <b><i>Ergebnisse: </i></b>Depressivität, Ängstlichkeit und Zwanghaftigkeit korrelieren positiv mit figur- und gewichtsbezogenen Sorgen. Bei Frauen mit einem niedrigeren Bildungsniveau war der Zusammenhang zwischen Depressivität und figur- und gewichtsbezogenen Sorgen höher als bei Frauen mit einem höheren Bildungsniveau. Berechnungen anhand linearer Regressionsanalysen zeigten, dass Depressivität und Zwanghaftigkeit die stärksten Prädiktoren für figur- und essensbezogene Sorgen für beide Bildungsgruppen waren. <b><i>Diskussion:</i></b> Wenngleich die Ergebnisse dieser Studie bei gesunden Frauen im mittleren Lebensalter erhoben wurden, gibt der beobachtete Zusammenhang zwischen negativer Affektivität, figur- und gewichtsbezogenen Sorgen und Bildung Hinweise auf eine Frühindikation bzw. Prävention von Essstörungen.