DOI: 10.5061/dryad.gtht76hx8
title: Data from: The extent of hybridization between bull trout (Salvelinus confluentus) and brook trout (S. fontinalis) across Alberta’s Eastern slopes

description: Identifying hybridization provides insight into risks associated with
 non-native species. Bull trout (Salvelinus confluentus), a charr native to
 Alberta, face population declines across their range and one risk may
 include hybridization with non-native brook trout (S. fontinalis).
 However, the extent of introgressive hybridization throughout Alberta is
 not well understood. Therefore, we aimed to quantify bull x brook trout
 hybridization in three ways: 1. the observed hybrid presence across
 Alberta, 2. the proportion of post-F1 hybrids (e.g., F2 and backcrossed),
 and 3. the hybrid proportion relative to brook trout in well-sampled
 watersheds. Using two panels of &gt;1,000 diagnostic Single Nucleotide
 Polymorphisms, we identified 35 F1 and 7 post-F1 hybrids (n = 42 total)
 across 10 HUC 8 watersheds. Our findings show hybridization throughout the
 bull trout range, however, with few post-F1 hybrids found, little evidence
 of extensive hybrid introgression was observed. The misidentification rate
 was low (2.1%), but 20 of 25 misidentifications involved confirmed
 hybrids, suggesting a higher misidentification rate (47.6%). This work
 improves the understanding of hybridization risks on at-risk bull trout in
 Alberta.
description: Genetic information on individual <em>Salvelinus
 spp.</em> samples (n=1200) collected across Alberta's Eastern
 Slopes. Files are data formatted for ADMIXTURE 1.3 software
 analyses.
description: # The extent of hybridization between bull trout (Salvelinus confluentus)
 and brook trout (S. fontinalis) across Alberta’s Eastern slopes
 [https://doi.org/10.5061/dryad.gtht76hx8](https://doi.org/10.5061/dryad.gtht76hx8) ## Description of the data and file structure All *Salvelinus spp.* fin clips were collected by Alberta Environment and Protected Areas (AEPA) staff between 1999 - 2022 using either electrofishing, angling, or trap netting as described in the manuscript. Samples spanned 24 Hydrologic Unit Code (HUC) 8 Watersheds in Alberta. The datasets contain Single Nucleotide Polymorphisms (SNPs) in ordinary PLINK format for analysis of ancestry estimates using ADMIXTURE 1.3 software (Alexander et al. 2009). ### Files and variables #### File: AB_hybridization_2025_nonBLT.map **Description:** Ordinary PLINK format file containing genetic map information on 1174 Diagnostic nonBLT (non-bull trout) loci. #### File: AB_hybridization_2025_BKT.map **Description:** Ordinary PLINK format file containing genetic map information on 1499 Diagnostic BKT (brook trout) loci. #### File: AB_hybridization_2025_nonBLT.ped **Description:** Ordinary PLINK format file containing HUC8 watershed (column 1), sample name (column 2), Father/Mother/Sex/Species (columns 3,4,5,6), and genotypes for 1174 nonBLT (non-bull trout) diagnostic loci (columns 7+, where columns 7 and 8 code the two alleles for diploid locus 1, and so on). #### File: AB_hybridization_2025_BKT.ped **Description:** Ordinary PLINK format file containing HUC 8 watershed (column 1), sample name (column 2), Father/Mother/Sex/Species (columns 3,4,5,6), and genotypes for 1499 BKT (brook trout) diagnostic loci (columns 7+, where columns 7 and 8 code the two alleles for diploid locus 1, and so on). ## Code/software Recommended Software for Data Analysis: * ADMIXURE 1.3 Software (ADMIXTURE manual: [https://dalexander.github.io/admixture/admixture-manual.pdf](https://dalexander.github.io/admixture/admixture-manual.pdf)). * PLINK 1.9 command-line program (for the conversion of .ped/.map to .bed file format see: [https://www.cog-genomics.org/plink/1.9/input](https://www.cog-genomics.org/plink/1.9/input)). ## References 1. Alexander, D. H., Novembre, J., and Lange, K. 2009. Fast model-based estimation of ancestry in unrelated individuals. *Genome Research*, 19(9): pp. 1655–1664.