DOI: 10.5061/dryad.tdz08kq5n
title: Data from: Metabolomic profiles of acute and chronic ambient hydrogen sulfide exposure in a mouse model

description: Hydrogen sulfide (H2S) is an environmental toxicant of health concern
 following acute or chronic human exposures. Male 6-8 week-old C57BL/6J
 mice were exposed by whole-body inhalation to 1000 ppm H2S for 45 min and
 euthanized at 5 min and 72 h for acute exposure. For subchronic study,
 mice were exposed to 5 ppm H2S 2 h/day, 5 days/week for 5 weeks. The
 brainstem was removed for metabolomic analysis. The metabolomics analyses
 consisted of three assays, (1) primary metabolism by GC-TOF MS, (2)
 biogenic amines (hydrophilic compounds) by HILIC-MS/MS and (3) lipidomics
 by RPLC-MS/MS. Metabolomics were performed in West Coast Metabolomics
 Center, University of California at Davis, CA, USA. 348, 311, and 565
 known metabolites were detected and analyzed by primary metabolism,
 biogenic amines, and lipidomic metabolomics assays. 33, 19, and 46
 metabolites were increased at 5 min and 72 h post acute H2S exposures and
 subchronic ambient H2S exposures, respectively, compared to room air
 control group. 22, 17, and 32 metabolites were decreased at 5 min and 72 h
 post acute H2S exposures and subchronic ambient H2S exposures,
 respectively, compared to room air control group. Acute H2S exposure
 decreased excitatory neurotransmitters aspartate and glutamate
 concentrations while the inhibitory neurotransmitter serotonin was
 increased. Glutamate and serotonin were also decreased after ambient H2S
 exposure. Branched-chain amino acids, fructose, and glucose were increased
 by acute H2S exposure. In ambient H2S exposure, glucose was decreased
 while MUFAs, PUFAs, inosine, and hypoxanthine were increased.
 Collectively, these results provide important mechanistic clues of acute
 and subchronic ambient H2S poisonings and show that H2S alters
 neurotransmission homeostasis.
description: Exposure paradigm<br>Hydrogen sulfide
 (H<sub>2</sub>S) is an environmental toxicant of health
 concern following acute or chronic human exposures. Male 6-8 week-old
 C57BL/6J mice were exposed by whole-body inhalation to 1000 ppm
 H<sub>2</sub>S for 45 min and euthanized at 5 min and 72 h for
 acute exposure. For subchronic study, mice were exposed to 5 ppm
 H<sub>2</sub>S 2 h/day, 5 days/week for 5 weeks. The brainstem
 was removed for metabolomic analysis.  Metabolomics
 analysis<br>The metabolomics analyses consisted of three assays, (1)
 primary metabolism by GC-TOF MS, (2) biogenic amines (hydrophilic
 compounds) by HILIC-MS/MS and (3) lipidomics by RPLC-MS/MS. Metabolomics
 were performed in West Coast Metabolomics Center (WCMC), University of
 California at Davis, CA, USA. Raw data were normalized by the standard
 normalization in WCMC. 
description: # Metabolomic profiles of acute and chronic ambient hydrogen sulfide
 exposure in a mouse model \# General information \## This README file was
 generated on 20204-01-04 by Dongsuk Kim. \## Author Information A.
 Principal Investigator Contact Information Name: Wilson Rumbeiha
 Institution: University of California at Davis Email:
 B. Associate or Co-investigator Contact
 Information Name: Dongsuk Kim Institution: University of California at
 Davis Email: \# Dataset contents: This data set
 contains 3 normalized metabolomics data in brainstem following acute and
 subchronic hydrogen sulfide exposures. \# SHARING/ACCESS INFORMATION 1\.
 Licenses/restrictions placed on the data: CC0 1.0 Universal (CC0 1.0)
 Public Domain 2\. Links to publications that cite or use the data:
 Dong-Suk Kim, Cristina MS Maldonado, Cecilia Giulivi, and Wilson K
 Rumbeiha (2024). Metabolomic signatures of brainstem in mice following
 acute and chronic hydrogen sulfide exposure. 3\. Links to other publicly
 accessible locations of the data: None 4\. Links/relationships to
 ancillary data sets: None 5\. Was data derived from another source? No 6\.
 Recommended citation for this dataset: Dong-Suk Kim, Cristina MS
 Maldonado, Cecilia Giulivi, and Wilson K Rumbeiha (2024). Data from:
 Metabolomic signatures of brainstem in mice following acute and chronic
 hydrogen sulfide exposure. Dryad Digital Repository.
 \# Experimental
 procedures: \## Exposure paradigm Hydrogen sulfide (H2S) is an
 environmental toxicant of health concern following acute or chronic human
 exposures. Male 6-8 week-old C57BL/6J mice were exposed by whole-body
 inhalation to 1000 ppm H2S for 45 min and euthanized at 5 min and 72 h for
 acute exposure. For subchronic study, mice were exposed to 5 ppm H2S 2
 h/day, 5 days/week for 5 weeks. The brainstem was removed for metabolomic
 analysis. \## Metabolomics analysis The metabolomics analyses consisted of
 three assays, (1) primary metabolism by GC-TOF MS, (2) biogenic amines
 (hydrophilic compounds) by HILIC-MS/MS and (3) lipidomics by RPLC-MS/MS.
 Metabolomics were performed in West Coast Metabolomics Center, University
 of California at Davis, CA, USA. \# Results: 348, 311, and 565 known
 metabolites were detected and analyzed by primary metabolism, biogenic
 amines, and lipidomic metabolomics assays. 33, 19, and 46 metabolites were
 increased at 5 min and 72 h post acute H2S exposures and subchronic
 ambient H2S exposures, respectively, compared to room air control group.
 22, 17, and 32 metabolites were decreased at 5 min and 72 h post acute H2S
 exposures and subchronic ambient H2S exposures, respectively, compared to
 room air control group. Acute H2S exposure decreased excitatory
 neurotransmitters aspartate and glutamate concentrations while the
 inhibitory neurotransmitter serotonin was increased. Glutamate and
 serotonin were also decreased after ambient H2S exposure. Branched-chain
 amino acids, fructose, and glucose were increased by acute H2S exposure.
 In ambient H2S exposure, glucose was decreased while MUFAs, PUFAs,
 inosine, and hypoxanthine were increased. Collectively, these results
 provide im-portant mechanistic clues of acute and subchronic ambient H2S
 poisonings and show that H2S alters neurotransmission homeostasis. \#
 Description of the data and file structure 1 PA data.txt contains
 normalized biogenic amines metabolomics data by biogenic amines assays. 2
 PM data.txt contains normalized primary metabolisom metabolomics data by
 biogenic amines assays. 3 Lipid data.txt contains normalized lipidomic
 metabolomics data by biogenic amines assays. \## DATA-SPECIFIC INFORMATION
 FOR: PA data.txt 1\. number of headers: 36 2\. number of data (Identified
 metabolites):309 3\. Header list: identifier: Index number of identified
 metabolite Metabolite name: Common name of identified metabolite Species:
 Chemical ionization mode InChiKey: International Chemical Identifier MSI
 level: Metabolomics Standards Initiative m/z: Mass-to-charge ratio RT:
 Retention time (minutes) A - 1: Peak height of Room air normal control
 group for 1000 ppm H2S exposure - 1 A - 2: Peak height of Room air normal
 control group for 1000 ppm H2S exposure - 2 A - 3: Peak height of Room air
 normal control group for 1000 ppm H2S exposure - 3 A - 4: Peak height of
 Room air normal control group for 1000 ppm H2S exposure - 4 A - 5: Peak
 height of Room air normal control group for 1000 ppm H2S exposure - 5 B -
 1: Peak height of 5 min post 1000 ppm H2S exposure - 1 B - 2: Peak height
 of 5 min post 1000 ppm H2S exposure - 2 B - 3: Peak height of 5 min post
 1000 ppm H2S exposure - 3 B - 4: Peak height of 5 min post 1000 ppm H2S
 exposure - 4 C - 1: Peak height of 72 h post 1000 ppm H2S exposure - 1 C -
 2: Peak height of 72 h post 1000 ppm H2S exposure - 2 C - 3: Peak height
 of 72 h post 1000 ppm H2S exposure - 3 C - 4: Peak height of 72 h post
 1000 ppm H2S exposure - 4 C - 5: Peak height of 72 h post 1000 ppm H2S
 exposure - 5 D - 1: Peak height of Room air normal control group for 5 ppm
 H2S exposure - 1 D - 2: Peak height of Room air normal control group for 5
 ppm H2S exposure - 2 D - 3: Peak height of Room air normal control group
 for 5 ppm H2S exposure - 3 D - 4: Peak height of Room air normal control
 group for 5 ppm H2S exposure - 4 E - 1: Peak height of 5 ppm H2S exposure
 - 1 E - 2: Peak height of 5 ppm H2S exposure - 2 E - 3: Peak height of 5
 ppm H2S exposure - 3 E - 4: Peak height of 5 ppm H2S exposure - 4 E - 5:
 Peak height of 5 ppm H2S exposure - 4 MtdBlank001: Peak height of Blank -
 1 MtdBlank002: Peak height of Blank - 2 MtdBlank003: Peak height of Blank
 - 3 PoolQC001: Peak height of Pooled quality control 1 PoolQC002: Peak
 height of Pooled quality control 2 PoolQC003: Peak height of Pooled
 quality control 3 4\. Abbreviation: na: Non-identified Inchikey \##
 DATA-SPECIFIC INFORMATION FOR: PM data.txt 1\. number of headers: 32 2\.
 number of data (Identified metabolites): 126 3\. Header list: Metabolite
 name: Name of identified metabolite ret.index: Retention time (minutes)
 quant mz: Mass-to-charge ratio mass spec: Mass spectra PubChem: ID of
 PubChem for the specific metabolite KEGG: ID of Kyoto Encyclopedia of
 Genes and Genomes InChI Key: International Chemical Identifier A - 1: Peak
 height of Room air normal control group for 1000 ppm H2S exposure - 1 A -
 2: Peak height of Room air normal control group for 1000 ppm H2S exposure
 - 2 A - 3: Peak height of Room air normal control group for 1000 ppm H2S
 exposure - 3 A - 4: Peak height of Room air normal control group for 1000
 ppm H2S exposure - 4 A - 5: Peak height of Room air normal control group
 for 1000 ppm H2S exposure - 5 B - 1: Peak height of 5 min post 1000 ppm
 H2S exposure - 1 B - 2: Peak height of 5 min post 1000 ppm H2S exposure -
 2 B - 3: Peak height of 5 min post 1000 ppm H2S exposure - 3 B - 4: Peak
 height of 5 min post 1000 ppm H2S exposure - 4 C - 1: Peak height of 72 h
 post 1000 ppm H2S exposure - 1 C - 2: Peak height of 72 h post 1000 ppm
 H2S exposure - 2 C - 3: Peak height of 72 h post 1000 ppm H2S exposure - 3
 C - 4: Peak height of 72 h post 1000 ppm H2S exposure - 4 C - 5: Peak
 height of 72 h post 1000 ppm H2S exposure - 5 D - 1: Peak height of Room
 air normal control group for 5 ppm H2S exposure - 1 D - 2: Peak height of
 Room air normal control group for 5 ppm H2S exposure - 2 D - 3: Peak
 height of Room air normal control group for 5 ppm H2S exposure - 3 D - 4:
 Peak height of Room air normal control group for 5 ppm H2S exposure - 4 E
 - 1: Peak height of 5 ppm H2S exposure - 1 E - 2: Peak height of 5 ppm H2S
 exposure - 2 E - 3: Peak height of 5 ppm H2S exposure - 3 E - 4: Peak
 height of 5 ppm H2S exposure - 4 E - 5: Peak height of 5 ppm H2S exposure
 - 4 pool_001: Peak height of Pooled quality control 1 pool_002: Peak
 height of Pooled quality control 2 4\. Abbreviation: na: Non-identified
 Pubchem or KEGG ID \## DATA-SPECIFIC INFORMATION FOR: Lipid data.txt 1\.
 number of headers: 38 2\. number of data (Identified metabolites): 565 3\.
 Header list: identifier: ID of identified metabolite name: Name of
 identified metabolite ion species: Ion species InChiKey: International
 Chemical Identifier m/z: Mass-to-charge ratio ESI mode: ESI mode ret.time:
 Retention time (minutes) A - 1: Peak height of Room air normal control
 group for 1000 ppm H2S exposure - 1 A - 2: Peak height of Room air normal
 control group for 1000 ppm H2S exposure - 2 A - 3: Peak height of Room air
 normal control group for 1000 ppm H2S exposure - 3 A - 4: Peak height of
 Room air normal control group for 1000 ppm H2S exposure - 4 A - 5: Peak
 height of Room air normal control group for 1000 ppm H2S exposure - 5 B -
 1: Peak height of 5 min post 1000 ppm H2S exposure - 1 B - 2: Peak height
 of 5 min post 1000 ppm H2S exposure - 2 B - 3: Peak height of 5 min post
 1000 ppm H2S exposure - 3 B - 4: Peak height of 5 min post 1000 ppm H2S
 exposure - 4 C - 1: Peak height of 72 h post 1000 ppm H2S exposure - 1 C -
 2: Peak height of 72 h post 1000 ppm H2S exposure - 2 C - 3: Peak height
 of 72 h post 1000 ppm H2S exposure - 3 C - 4: Peak height of 72 h post
 1000 ppm H2S exposure - 4 C - 5: Peak height of 72 h post 1000 ppm H2S
 exposure - 5 D - 1: Peak height of Room air normal control group for 5 ppm
 H2S exposure - 1 D - 2: Peak height of Room air normal control group for 5
 ppm H2S exposure - 2 D - 3: Peak height of Room air normal control group
 for 5 ppm H2S exposure - 3 D - 4: Peak height of Room air normal control
 group for 5 ppm H2S exposure - 4 E - 1: Peak height of 5 ppm H2S exposure
 - 1 E - 2: Peak height of 5 ppm H2S exposure - 2 E - 3: Peak height of 5
 ppm H2S exposure - 3 E - 4: Peak height of 5 ppm H2S exposure - 4 E - 5:
 Peak height of 5 ppm H2S exposure - 4 PoolQC001: Peak height of Pooled
 quality control 1 PoolQC002: Peak height of Pooled quality control 2
 PoolQC003: Peak height of Pooled quality control 3 PoolQC004: Peak height
 of Pooled quality control 4 MtdBlank001: Peak height of Blank - 1
 MtdBlank002: Peak height of Blank - 2 MtdBlank003: Peak height of Blank -
 3 MtdBlank004: Peak height of Blank - 4 Missing data code: na