cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7516520
title: MORPHOLOGIGAL MEASUREMENTS OF LYMPHOID TISSUES AND INTESTINAL DEVELOPMENT OF CHICKS FROM DIFFERENT BREEDER AGES AND HATCHED UNDER HEAT STRESS

description: Abstract: This study was carried out to evaluate the effect of breeder age and hatching temperature variations on chick quality. The experiment was a completely randomized design in a 3x2 factorial arrangement (three breeder ages and two hatching temperatures). We used 720 eggs from three breeder ages (30, 42, or 64 weeks). The hatching temperature for control group was 37.5 ºC during all the hatching period and for the elevated temperature group, it was 39.0 ºC during 6h between 10 and 18 days of incubation. Five chicks per treatment were euthanized by cervical dislocation for bursal and spleen morphological measurements. The data were analyzed using ANOVA and the differences among means were compared by Tukey test (5%). Chicks from older flock had larger follicular area of bursa de Fabricius when hatched under normal temperature. The higher hatching temperature induced intestinal development because chicks hatched under high temperature had bigger crypt depth. Changes in hatching machine temperature affected young flock chick quality. Chicks from older flocks had better immune response.
DOI: 10.6084/m9.figshare.7516505
title: THE EFFECT OF THE INTENSITY OF ESTRUS EXPRESSION ON THE FOLLICULAR DIAMETER AND FERTILITY OF NELLORE COWS MANAGED UNDER A FTAI PROGRAM

description: Abstract The aim of this study was to determine the relationship between the intensity of estrus expression on the ovulatory follicle diameter (OFD) and its effect on the conception rate in zebu females managed under a FTAI program. On a random day of the estrous cycle, day 0, 308 Nellore females received an intravaginal progesterone device and 2.0 mg of estradiol benzoate were administered intramuscularly (IM). On day 8, the progesterone device was removed, and 300 IU of equine chorionic gonadotropin, 150 µg of d-cloprostenol, and 1.0 mg of estradiol cypionate were administered IM. The animals were then painted with animal marker spray between the sacral tuberosity and the tailhead. On day 10, the animals were categorized into three groups: no estrus expression (n = 78), low intensity of estrus expression (n = 66) and high intensity of estrus expression (n = 164). The OFD was then measured, and artificial insemination (AI) was performed. The results suggest that determining the intensity of estrus expression is a good parameter to identify females with greater follicular diameter and increased fertility in Nellore cows submitted to a FTAI protocol.
DOI: 10.6084/m9.figshare.7516520.v1
title: MORPHOLOGIGAL MEASUREMENTS OF LYMPHOID TISSUES AND INTESTINAL DEVELOPMENT OF CHICKS FROM DIFFERENT BREEDER AGES AND HATCHED UNDER HEAT STRESS

description: Abstract: This study was carried out to evaluate the effect of breeder age and hatching temperature variations on chick quality. The experiment was a completely randomized design in a 3x2 factorial arrangement (three breeder ages and two hatching temperatures). We used 720 eggs from three breeder ages (30, 42, or 64 weeks). The hatching temperature for control group was 37.5 ºC during all the hatching period and for the elevated temperature group, it was 39.0 ºC during 6h between 10 and 18 days of incubation. Five chicks per treatment were euthanized by cervical dislocation for bursal and spleen morphological measurements. The data were analyzed using ANOVA and the differences among means were compared by Tukey test (5%). Chicks from older flock had larger follicular area of bursa de Fabricius when hatched under normal temperature. The higher hatching temperature induced intestinal development because chicks hatched under high temperature had bigger crypt depth. Changes in hatching machine temperature affected young flock chick quality. Chicks from older flocks had better immune response.
DOI: 10.6084/m9.figshare.7516505.v1
title: THE EFFECT OF THE INTENSITY OF ESTRUS EXPRESSION ON THE FOLLICULAR DIAMETER AND FERTILITY OF NELLORE COWS MANAGED UNDER A FTAI PROGRAM

description: Abstract The aim of this study was to determine the relationship between the intensity of estrus expression on the ovulatory follicle diameter (OFD) and its effect on the conception rate in zebu females managed under a FTAI program. On a random day of the estrous cycle, day 0, 308 Nellore females received an intravaginal progesterone device and 2.0 mg of estradiol benzoate were administered intramuscularly (IM). On day 8, the progesterone device was removed, and 300 IU of equine chorionic gonadotropin, 150 µg of d-cloprostenol, and 1.0 mg of estradiol cypionate were administered IM. The animals were then painted with animal marker spray between the sacral tuberosity and the tailhead. On day 10, the animals were categorized into three groups: no estrus expression (n = 78), low intensity of estrus expression (n = 66) and high intensity of estrus expression (n = 164). The OFD was then measured, and artificial insemination (AI) was performed. The results suggest that determining the intensity of estrus expression is a good parameter to identify females with greater follicular diameter and increased fertility in Nellore cows submitted to a FTAI protocol.
DOI: 10.6084/m9.figshare.7515998
title: ECONOMIC DETERMINISTIC ANALYSIS OF TWO YEARS OLD STEERS PRODUCTION SYSTEMS

description: Abstract The objective of this study was to realize the economic deterministic analysis of steers production system in full cycle, finished in feedlot or cultivated pasture at twenty-four months old, utilizing or not the quality bonus from slaughterhouses. The study was realized using data from meta-analytic survey of studies in the central region of Rio Grande do Sul state, Brazil, in Microsoft Excel® spreadsheets. The systems were simulated considering the male and female calves weaned early, at the tree months of age, and finished at the 24 months of age in feedlot or cultivated pasture. After the slaughter, it was realized the economic deterministic analysis. According to financial return indicators net present value (NPV), benefit:cost index (IB:C) and additional return on investment (ROI) only the system in pasture show return at the end of planning horizon, and the internal return rate (IRR), modified internal return rate (MIRR) and discounted payback (PBd), show that this system possesses a higher risk of investment. The feedlot system show negative results as a investment project. The bigger cost center was depreciation, feeding supply and taxes, and the quality bonus shows up an interesting alternative of return, but was not sufficient to render the feedlot system a viable project.
DOI: 10.6084/m9.figshare.7515998.v1
title: ECONOMIC DETERMINISTIC ANALYSIS OF TWO YEARS OLD STEERS PRODUCTION SYSTEMS

description: Abstract The objective of this study was to realize the economic deterministic analysis of steers production system in full cycle, finished in feedlot or cultivated pasture at twenty-four months old, utilizing or not the quality bonus from slaughterhouses. The study was realized using data from meta-analytic survey of studies in the central region of Rio Grande do Sul state, Brazil, in Microsoft Excel® spreadsheets. The systems were simulated considering the male and female calves weaned early, at the tree months of age, and finished at the 24 months of age in feedlot or cultivated pasture. After the slaughter, it was realized the economic deterministic analysis. According to financial return indicators net present value (NPV), benefit:cost index (IB:C) and additional return on investment (ROI) only the system in pasture show return at the end of planning horizon, and the internal return rate (IRR), modified internal return rate (MIRR) and discounted payback (PBd), show that this system possesses a higher risk of investment. The feedlot system show negative results as a investment project. The bigger cost center was depreciation, feeding supply and taxes, and the quality bonus shows up an interesting alternative of return, but was not sufficient to render the feedlot system a viable project.
DOI: 10.6084/m9.figshare.7516523
title: STRUCTURE AND PRODUCTIVITY OF TANZANIA GRASS SUBJECTED TO DIFFERENT DEFOLIATION FREQUENCIES

description: Abstract The objective of the present study was to evaluate the effect of different frequencies of defoliation at fixed periods on the structural and productive characteristics of Tanzania grass (Panicum maximum) under Am climate conditions in 2014. The experimental design was in complete randomized blocks with six treatments corresponding to six frequencies of defoliation (14, 21, 28, 35, 42, and 49 days) and five repetitions. The data was submitted to regression analysis. The light interception (LI) increased (P &lt;0.05) in an asymptotic way with increasing rest periods. The maximum light interception was 98% and occurred at 42 days of regrowth. The leaf area index (LAI) and the dry matter accumulation increased (P &lt;0.05) linearly varying from 3.6 to 7.1 and from 880.3 to 4051.7 kg.ha-1 of forage dry matter in the frequencies 14-49 days, respectively. The height ranged 44-119 cm at frequencies 14-49 days, respectively. The frequency of defoliation at 95% LI is related to the rest period of 29 days, where the Tanzania grass canopy presented around 63 cm height, critical LAI of 5.1, and forage dry matter accumulation of 2,160 kg ha-1.
DOI: 10.6084/m9.figshare.7516523.v1
title: STRUCTURE AND PRODUCTIVITY OF TANZANIA GRASS SUBJECTED TO DIFFERENT DEFOLIATION FREQUENCIES

description: Abstract The objective of the present study was to evaluate the effect of different frequencies of defoliation at fixed periods on the structural and productive characteristics of Tanzania grass (Panicum maximum) under Am climate conditions in 2014. The experimental design was in complete randomized blocks with six treatments corresponding to six frequencies of defoliation (14, 21, 28, 35, 42, and 49 days) and five repetitions. The data was submitted to regression analysis. The light interception (LI) increased (P &lt;0.05) in an asymptotic way with increasing rest periods. The maximum light interception was 98% and occurred at 42 days of regrowth. The leaf area index (LAI) and the dry matter accumulation increased (P &lt;0.05) linearly varying from 3.6 to 7.1 and from 880.3 to 4051.7 kg.ha-1 of forage dry matter in the frequencies 14-49 days, respectively. The height ranged 44-119 cm at frequencies 14-49 days, respectively. The frequency of defoliation at 95% LI is related to the rest period of 29 days, where the Tanzania grass canopy presented around 63 cm height, critical LAI of 5.1, and forage dry matter accumulation of 2,160 kg ha-1.
DOI: 10.6084/m9.figshare.7513238
title: HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

description: Abstract The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI95 17.7% - 30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI95 40.0% - 59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P&gt;0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P&lt;0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P&gt;0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis.
DOI: 10.6084/m9.figshare.7513238.v1
title: HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

description: Abstract The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI95 17.7% - 30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI95 40.0% - 59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P&gt;0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P&lt;0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P&gt;0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis.
DOI: 10.25345/c5tr42
title: MassIVE MSV000087113 - A novel regulatory mechanism of U2 spliceosome proteostasis that controls the transcriptome and therapy response in cancer
title: Aberrant splicing is an important cause of cancer, and the production of noncanonical, cancer-specific, mRNA transcripts associates with transformation. Studies have focused on characterizing the effect of spliceosome mutations on disease progression. Here, we have uncovered a novel mode of regulation of the U2 complex component SF3B1 in leukemia via lysosomal degradation. Elevated levels of SF3B1 protein control splicing and active transcription via direct interaction with the general transcriptional machinery and are critical for leukemia growth due to the control of cell cycle and DNA damage response-related transcripts, such as the serine/threonine kinase CHEK2. We further exploited these findings to show that clinically-used SF3B1 inhibitors synergize with transcriptional inhibitors, CHEK2 inhibitors and chemotherapy drugs to block leukemia growth. Our study provides the proof-of-principle for post-translational regulation of the splicing machinery via the lysosome and associated splicing-dependent and -independent roles of the U2 complex in cancer, that can be exploited for therapeutic purposes.
DOI: 10.6084/m9.figshare.7513256
title: EFFECTS OF MIDAZOLAM IN DIFFERENT DOSES IN REDTAIL BOA Boa constrictor LINNAEUS, 1758 (SQUAMATA: BOIDAE)

description: Abstract The objective of this study was to compare the effects of midazolam in Boa constrictor. We used 20 redtail boas, divided into two groups of ten animals each. Group 1 (G1) received 1 mg/kg of midazolam and group 2 (G2) 2 mg/kg, by intracelomic injection. The specimens of G1 presented reduction of head tone, muscle tone, manipulation, and locomotion for 233.50 ± 71.34 minutes and the representatives of G2 for 328.50 ± 125.35 minutes. No redtail boa belonging to both G1 and G2 presented absence of postural righting reflex or achieved analgesia. However, all specimens tested showed intense muscle relaxation and difficulty to move in. The turnaround time to pre-sedation conditions were 279 ± 73.55 minutes for G1 and 372 ± 142.27 minutes for G2. There were no statistical differences between evaluations of heart and respiratory rates in both groups tested. We concluded, therefore, in redtail boa, midazolam at the dosage of 1 mg/kg causes the same effect as 2 mg/kg, but with shorter recovery time, and it can be used to contain Boa constrictor or in associations aimed at an effective muscle relaxation.
DOI: 10.6084/m9.figshare.7513256.v1
title: EFFECTS OF MIDAZOLAM IN DIFFERENT DOSES IN REDTAIL BOA Boa constrictor LINNAEUS, 1758 (SQUAMATA: BOIDAE)

description: Abstract The objective of this study was to compare the effects of midazolam in Boa constrictor. We used 20 redtail boas, divided into two groups of ten animals each. Group 1 (G1) received 1 mg/kg of midazolam and group 2 (G2) 2 mg/kg, by intracelomic injection. The specimens of G1 presented reduction of head tone, muscle tone, manipulation, and locomotion for 233.50 ± 71.34 minutes and the representatives of G2 for 328.50 ± 125.35 minutes. No redtail boa belonging to both G1 and G2 presented absence of postural righting reflex or achieved analgesia. However, all specimens tested showed intense muscle relaxation and difficulty to move in. The turnaround time to pre-sedation conditions were 279 ± 73.55 minutes for G1 and 372 ± 142.27 minutes for G2. There were no statistical differences between evaluations of heart and respiratory rates in both groups tested. We concluded, therefore, in redtail boa, midazolam at the dosage of 1 mg/kg causes the same effect as 2 mg/kg, but with shorter recovery time, and it can be used to contain Boa constrictor or in associations aimed at an effective muscle relaxation.
DOI: 10.6084/m9.figshare.7513244
title: VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS

description: Abstract The occurrence of viable cells in mammary gland secretions attests the functionality of the defense system. This fact, along with applied medicine, is fundamental for the healing udder. The objective of this study was to evaluate the in vitro viability of milk leukocytes challenged with four intramammary anti-mastitis drugs. We collected eight cow milk samples that were negative after a bacteriological examination. Cell suspensions were submitted to an evaluation of viability through Trypan Blue exclusion before and after incubation with the drugs. Mean pre-incubation viability rate (± standard deviation) of milk cells was 50.17% (± 6.50). Mean post-incubation viability rates (± standard deviation) of control group (devoid of any drug), Drug 1 (anhydrous cephalonium), Drug 2 (gentamicin), Drug 3 (procaine benzylpenicillin associated with dihydrostreptomycin nafcillin), and Drug 4 (benzathine cloxacillin) were, respectively, 31.36% (± 3.61); 26.23% (± 4.49); 7.82% (± 2.33); 3.10% (± 0.94); and 30.33% (± 4.14). The uppermost impairments were found when milk cells were challenged with Drug 2 and Drug 3. Although antimicrobial activity of tested drugs was not appraised, and a single aspect of cell function was tested, we concluded that treatments 2 and 3 could impair the functionality of milk leukocytes and could provide an ineffective immunological response, and an effective response is essential to control mammary infections.
DOI: 10.6084/m9.figshare.7513244.v1
title: VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS

description: Abstract The occurrence of viable cells in mammary gland secretions attests the functionality of the defense system. This fact, along with applied medicine, is fundamental for the healing udder. The objective of this study was to evaluate the in vitro viability of milk leukocytes challenged with four intramammary anti-mastitis drugs. We collected eight cow milk samples that were negative after a bacteriological examination. Cell suspensions were submitted to an evaluation of viability through Trypan Blue exclusion before and after incubation with the drugs. Mean pre-incubation viability rate (± standard deviation) of milk cells was 50.17% (± 6.50). Mean post-incubation viability rates (± standard deviation) of control group (devoid of any drug), Drug 1 (anhydrous cephalonium), Drug 2 (gentamicin), Drug 3 (procaine benzylpenicillin associated with dihydrostreptomycin nafcillin), and Drug 4 (benzathine cloxacillin) were, respectively, 31.36% (± 3.61); 26.23% (± 4.49); 7.82% (± 2.33); 3.10% (± 0.94); and 30.33% (± 4.14). The uppermost impairments were found when milk cells were challenged with Drug 2 and Drug 3. Although antimicrobial activity of tested drugs was not appraised, and a single aspect of cell function was tested, we concluded that treatments 2 and 3 could impair the functionality of milk leukocytes and could provide an ineffective immunological response, and an effective response is essential to control mammary infections.
DOI: 10.6084/m9.figshare.7508867
title: MAMMARY BIOPSY IN INDUCED LACTATING GOATS: ASSESSMENT OF POST-BIOPSY MILK PROPERTIES AND ULTRASONOGRAPHIC APPEARANCE

description: Abstract The aim of this study was to evaluate the effects of mammary biopsy procedures on gland function of goats submitted to hormonal lactation. Ten female Caninde goats, at the age of around 3 years, were used, and lactation was induced by using estrogen, progesterone, and prednisolone. Ultrasonographic examinations of mammary gland, milking, and analysis of physicochemical properties of milk were performed immediately before (0 h) and 24, 48, and 72 h after each biopsy procedure. Surgical mammary gland biopsies were obtained at days 5 and 26 of the lactation. Even after drying initiation, all animals produced milk for five months. No abnormalities were observed on the ultrasonographic appearance of biopsied glands. All 20 biopsies were technically successful and any inflammation or infection was associated with biopsy procedures. Regarding the physicochemical properties and volumes of milk produced, no significant differences (P &gt; 0.05) were observed between right and left glands nor in the comparison of the results from time 0 with 24, 48, and 72 h for biopsied teats. The mammary biopsy did not induce neither severe changes in production and physicochemical properties of the milk nor in ultrasonographic appearance of mammary gland of goats in hormonal lactation.
DOI: 10.6084/m9.figshare.7508867.v1
title: MAMMARY BIOPSY IN INDUCED LACTATING GOATS: ASSESSMENT OF POST-BIOPSY MILK PROPERTIES AND ULTRASONOGRAPHIC APPEARANCE

description: Abstract The aim of this study was to evaluate the effects of mammary biopsy procedures on gland function of goats submitted to hormonal lactation. Ten female Caninde goats, at the age of around 3 years, were used, and lactation was induced by using estrogen, progesterone, and prednisolone. Ultrasonographic examinations of mammary gland, milking, and analysis of physicochemical properties of milk were performed immediately before (0 h) and 24, 48, and 72 h after each biopsy procedure. Surgical mammary gland biopsies were obtained at days 5 and 26 of the lactation. Even after drying initiation, all animals produced milk for five months. No abnormalities were observed on the ultrasonographic appearance of biopsied glands. All 20 biopsies were technically successful and any inflammation or infection was associated with biopsy procedures. Regarding the physicochemical properties and volumes of milk produced, no significant differences (P &gt; 0.05) were observed between right and left glands nor in the comparison of the results from time 0 with 24, 48, and 72 h for biopsied teats. The mammary biopsy did not induce neither severe changes in production and physicochemical properties of the milk nor in ultrasonographic appearance of mammary gland of goats in hormonal lactation.