cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/h4bwj-09418
title: Hippocampal Neurons (cultured)

description: These tomograms are correlated with low mag images of neurites leaving the cell body. They were collected to make sure I had tomograms near the proximal regions of neurites.
description: <strong>Tilt Series Date:</strong> 2014-12-05</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-05-1/rawdata/</p>120514_hipp_neuron_square1_tomo1.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-05-1/rawdata/120514_hipp_neuron_square1_tomo1.mrc"
        > <i class="download icon"></i> Download </a></p>

        120514_hipp_neuron_square1_tomo1_part121_3.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-05-1/3dimage_48603/120514_hipp_neuron_square1_tomo1_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/marqq-6ch20
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-92/rawdata/</p>RCC745_RELtovis_101.mrc,  Tilt Series (Pixel Size 1.26 nm), 570.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-92/rawdata/RCC745_RELtovis_101.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_101.rec,  Reconstruction (Pixel Size 1.26 nm), 501.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-92/3dimage_12525/RCC745_RELtovis_101.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.pqgwuq
title: Occurrence Download

description: A dataset containing 410 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sigesbeckia orientalis",
 "Country is Brazil",
 "HasCoordinate is true"
 ]
}

 The dataset includes 410 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048293-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/hey68-r0e18
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-07-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-45/3dimage_11517/</p>RCC745_MGtovis_178.rec,  Reconstruction (Pixel Size 1.26 nm), 129.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-45/3dimage_11517/RCC745_MGtovis_178.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_178.mrc,  Tilt Series (Pixel Size 1.26 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-45/rawdata/RCC745_MGtovis_178.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/35j48-w3f54
title: Skinny E. coli with GFP-MreB

description: Raw data files of Skinny E. coli with GFP-MreB
description: <strong>Tilt Series Date:</strong> 2010-03-05</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR and Peach</p>
<strong>Collaborators and Roles:</strong> Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)</p>
<strong>Sample Preparation:</strong> R2/2 CF or Quantifoil, 10 nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-21/rawdata/</p>10mar05a_ZL001_021.mrc,  Tilt Series (Pixel Size 1.192 nm), 721.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-21/rawdata/10mar05a_ZL001_021.mrc"
        > <i class="download icon"></i> Download </a></p>

        10mar05a_ZL001_021_full.rec,  Reconstruction (Pixel Size 2.384 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-21/3dimage_9327/10mar05a_ZL001_021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hjq837
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sigesbeckia orientalis",
 "Country is Belgium",
 "HasCoordinate is true"
 ]
}

 The dataset includes 3 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048292-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/79fbr-yjb19
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-21/3dimage_11653/</p>RCC745_MGtovis_334.rec,  Reconstruction (Pixel Size 1.26 nm), 239.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-21/3dimage_11653/RCC745_MGtovis_334.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_334.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-21/rawdata/RCC745_MGtovis_334.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rcjba-gyz19
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-11-16</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-72/rawdata/</p>RCC745_MGtovis_483.mrc,  Tilt Series (Pixel Size 1.26 nm), 738.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-72/rawdata/RCC745_MGtovis_483.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_483.rec,  Reconstruction (Pixel Size 1.26 nm), 166.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-72/3dimage_11909/RCC745_MGtovis_483.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16956038
title: Agarwal et al. (2025) Joint Library Dataset (56k sequences) Evaluator Container for the Genomic API for Model Evaluation (GAME)

description: This container is the Evaluator configured for the Genomic API for Model Evaluation (GAME), designed specifically to evaluate model predictions against the Agarwal et al. (2025) Joint Library dataset. The dataset consists of 56,982 candidate cis-regulatory elements (cCREs), including enhancers and promoters systematically tested across:



 induced pluripotent stem cells (iPS cells; WTC11)

lymphoblasts (K562)

human hepatocytes (HepG2)


The dataset also includes positive and negative control sequences.

Evaluation Workflow





Expression Prediction





Evaluates Predictor performance for expression (point predictions).




Computes the Pearson correlation between measured values (log2(rna/dna)) and predicted values.






Cell Type Specificity





Evaluates cell type specificity between measured and predicted values by calculating the pearson correlation between:





[cell1_measured - cell2_measured] and [cell1_predicted - cell2_predicted].







Included in agarwal_joint_56k.sif:



Scripts to process the data and connect to predictors in the GAME API

Scripts to parse the returned predictions and calculate performance metrics

All software dependencies


/evaluator_data folder contents:



2023-03-03628C-Table_S10-joint_lib_design_56k_measured.xlsx: Sequence design and coordinate information (each sequence is a 230-bp oligonucleotide)

all_cell_type_measured.xlsx: Measurments for the ~56k sequences across all 3 cell types [log2(rna/dna)]


How to run:

apptainer run --containall -B /path_to/evaluator_data/:/evaluator_data -B /path_to/prediction_folder/:/predictions agarwal_joint_56k.sif HOST PORT /predictions

Notes:



Details about QC filtering can be found in the original publication

The original 2023-03-03628C-Table_S10-joint_lib_design.xlsx sequence file from the paper was filtered to only keep sequences with measured values in all_cell_type_measured.xlsx

When evaluating expression predictions for each cell type, the number of measurements used for Pearson correlation may vary due to filtering.

Cell type specificity evaluation uses only sequences with measurements across all three cell types to ensure fairness


Additional information can be found on GitHub: Genomic API for Model Evaluation

Original publication can be found here: Agarwal et al. 2025
DOI: 10.5281/zenodo.16956039
title: Agarwal et al. (2025) Joint Library Dataset (56k sequences) Evaluator Container for the Genomic API for Model Evaluation (GAME)

description: This container is the Evaluator configured for the Genomic API for Model Evaluation (GAME), designed specifically to evaluate model predictions against the Agarwal et al. (2025) Joint Library dataset. The dataset consists of 56,982 candidate cis-regulatory elements (cCREs), including enhancers and promoters systematically tested across:



 induced pluripotent stem cells (iPS cells; WTC11)

lymphoblasts (K562)

human hepatocytes (HepG2)


The dataset also includes positive and negative control sequences.

Evaluation Workflow





Expression Prediction





Evaluates Predictor performance for expression (point predictions).




Computes the Pearson correlation between measured values (log2(rna/dna)) and predicted values.






Cell Type Specificity





Evaluates cell type specificity between measured and predicted values by calculating the pearson correlation between:





[cell1_measured - cell2_measured] and [cell1_predicted - cell2_predicted].







Included in agarwal_joint_56k.sif:



Scripts to process the data and connect to predictors in the GAME API

Scripts to parse the returned predictions and calculate performance metrics

All software dependencies


/evaluator_data folder contents:



2023-03-03628C-Table_S10-joint_lib_design_56k_measured.xlsx: Sequence design and coordinate information (each sequence is a 230-bp oligonucleotide)

all_cell_type_measured.xlsx: Measurments for the ~56k sequences across all 3 cell types [log2(rna/dna)]


How to run:

apptainer run --containall -B /path_to/evaluator_data/:/evaluator_data -B /path_to/prediction_folder/:/predictions agarwal_joint_56k.sif HOST PORT /predictions

Notes:



Details about QC filtering can be found in the original publication

The original 2023-03-03628C-Table_S10-joint_lib_design.xlsx sequence file from the paper was filtered to only keep sequences with measured values in all_cell_type_measured.xlsx

When evaluating expression predictions for each cell type, the number of measurements used for Pearson correlation may vary due to filtering.

Cell type specificity evaluation uses only sequences with measurements across all three cell types to ensure fairness


Additional information can be found on GitHub: Genomic API for Model Evaluation

Original publication can be found here: Agarwal et al. 2025
DOI: 10.22002/1zwca-0vm43
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC114
description: <strong>Tilt Series Date:</strong> 2014-08-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-21/rawdata/</p>20140811_YWC114_30011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-21/rawdata/20140811_YWC114_30011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140811_YWC114_30011_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-21/3dimage_45461/20140811_YWC114_30011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e6m5m-qch27
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC115
description: <strong>Tilt Series Date:</strong> 2014-09-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 3 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Wait time 10 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-30/rawdata/</p>20140915_YWC115_30022.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-30/rawdata/20140915_YWC115_30022.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140915_YWC115_30022_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-30/3dimage_46284/20140915_YWC115_30022_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r5nxp-dy663
title: Myxococcus xanthus delta-pilC

description: grid box YWC060
description: <strong>Tilt Series Date:</strong> 2013-12-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-19/rawdata/</p>20131226_Janelia_YWC060_10044.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-19/rawdata/20131226_Janelia_YWC060_10044.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131226_Janelia_YWC060_10044_part131_1.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-19/3dimage_37098/20131226_Janelia_YWC060_10044_part131_1.rec"
        > <i class="download icon"></i> Download </a></p>