DOI: 10.5061/dryad.18g80
title: Data from: Convergent and correlated evolution of major life-history traits in the angiosperm genus Leucadendron (Proteaceae)

description: Natural selection is expected to cause convergence of life histories among
 taxa as well as correlated evolution of different life-history traits.
 Here, we quantify the extent of convergence of five key life-history
 traits (adult fire survival, seed storage, degree of sexual dimorphism,
 pollination mode, and seed-dispersal mode) and test hypotheses about their
 correlated evolution in the genus Leucadendron (Proteaceae) from the
 fire-prone South African fynbos. We reconstructed a new molecular
 phylogeny of this highly diverse genus that involves more taxa and
 molecular markers than previously. This reconstruction identifies new
 clades that were not detected by previous molecular study and
 morphological classifications. Using this new phylogeny and robust methods
 that account for phylogenetic uncertainty, we show that the five
 life-history traits studied were labile during the evolutionary history of
 the genus. This diversity allowed us to tackle major questions about the
 correlated evolution of life-history strategies. We found that species
 with longer seed-dispersal distances tended to evolve lower
 pollen-dispersal distance, that insect-pollinated species evolved
 decreased sexual dimorphism, and that species with a persistent soil
 seed-bank evolved toward reduced fire-survival ability of adults.
description: DNA alignment for the
 genus Leucadendron phylogenyThis alignment is the
 concatenation of nine markers that were used to reconstruct the phylogeny
 of the genus Leucadendron in the paper cited above. Those markers were
 amplified for 81 Leucadendron taxa collected in natural populations in the
 South-African fynbos, except for three external groups which were
 collected in the Kirstenbosch Botanical Garden: Paranomus spathulatus,
 Mimetes cucullatus and Leucospermum erubescens. A detailed map of the
 alignment is given below: Positions 1 - 618 : AS1 marker which contains an
 exon of 618bp. Positions 619 - 1046 : PPR-like marker which contains an
 exon of 233bp and a 3'-UTR of 195bp. Positions 1047 - 1800 : SVR7
 marker which contains an exon of 652bp and a 3'-UTR of 102bp.
 Positions 1801 - 2181 : CAF1-6 marker which only contains an exon of
 381bp. Positions 2182 - 2795 : the classical ITS marker. Positions 2796 -
 3591 : ATINT1 marker which contains an exon of 665bp and a 3'-UTR of
 131bp. Positions 3592 - 3868 : APO2 marker which contains only an exon of
 277bp. Positions 3869 - 4207 : APG6 marker which contains only an exon of
 339bp. Positions 3208 - 4865 : ATPHAN marker which contains only an exon
 of 658bp The methodology used to design the following markers: PPR-like,
 CAF1-6, ATINT1, APO2, APG6 and ATPHAN is described in (Tonnabel et al.
 2013). The AS1 marker was designed in (Illing et al. 2009). Tonnabel, J.,
 Olivieri, I., Mignot, A., Rebelo, A., Justy, F., Santoni, S., Caroli, S.,
 Sauné, L., Bouchez, O. & Douzery, E.J.P. (2014) Developing nuclear DNA
 phylogenetic markers in the angiosperm genus Leucadendron (Proteaceae): a
 next-generation sequencing transcriptomic approach, Molecular
 Phylogenetics and Evolution, 70, 37-46. Illing, N., Klak, C., Johnson, C.,
 Brito, D., Negrao, N., Baine, F., van Kets, V., Ramchurn K.R., Seoighe, C.
 & Roden, L. (2009) Duplication of the Asymmetric Leaves1/Rough Sheath
 2/Phantastica (ARP) gene precedes the explosive radiation of the
 Ruschioideae. Development genes and evolution, 219,
 331-338.Tonnabeletal_Evolution_alignment_Leucadendron.fasdata_sexspecific_leaf_measurementsThis dataset corresponds to the sex-specific leaves measurements realized for the Leucadendron genus (L.) in order to assess sexual dimorphism at the leave level. Leaf area (named area), leaf length (named length) and leaf width (named width) have been assessed for several individuals (ind) of several populations (pop) of different species (species). Within the file, females are designed by 'F' and males by 'M'.data_sexspecific_leaf_measurement.ods