cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.t24g8a
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ranunculus obscurisepalus (Julin) Ericsson"
 ]
}

 The dataset includes 13 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090805-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dsqk6u
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rhododendron cravenii Danet"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090808-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7bprup
title: Occurrence Download

description: A dataset containing 35 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rhynchosia bullata (E.Mey.) Benth. ex Harv."
 ]
}

 The dataset includes 35 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090809-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ztgzyb
title: Occurrence Download

description: A dataset containing 282 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Viola selkirkii Pursh ex Goldie",
 "Country is China",
 "Year is greater than or equal to 1900",
 "HasCoordinate is true"
 ]
}

 The dataset includes 282 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090811-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nnnbn6
title: Occurrence Download

description: A dataset containing 44 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Oxalis triangularis A.St.-Hil.",
 "Country is China",
 "Year is greater than or equal to 1900",
 "HasCoordinate is true"
 ]
}

 The dataset includes 44 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090813-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7e2kjf
title: Occurrence Download

description: A dataset containing 250 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Pyrrosia heteractis (Mett.) Ching"
 ]
}

 The dataset includes 250 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090810-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.20496369
title: <i>Varroa destructor</i> in Portugal: an exploratory assessment of pyrethroids resistance status

description: The drastic and abrupt loss of honey bee colonies around the world has long unsettled the beekeeping community, and although the phenomenon is far from being solved, there is a relative consensus regarding the detrimental effect the mite <i>Varroa destructor</i> exerts over <i>Apis mellifera</i> populations. In Portugal, this parasite was first recorded in the late 1980s and since then, the beekeepers’ method of choice for controlling the mite are synthetic acaricides, most notably the pyrethroids tau-fluvalinate and flumethrin. Unsurprisingly, resistance to these active ingredients quickly surfaced. Here, we report the first PCR-RFLP resistance assessment in Portuguese apiaries, where 96 <i>V. destructor</i> mites, from different geographic regions, were genotyped between April and August of 2019. The results showed that 47% of the sampled mites exhibit the mutation correlated with resistance. These findings stress the need for a current characterization of the acaricides being used and a description of how the treatments are been applied.
DOI: 10.6084/m9.figshare.20496369.v1
title: <i>Varroa destructor</i> in Portugal: an exploratory assessment of pyrethroids resistance status

description: The drastic and abrupt loss of honey bee colonies around the world has long unsettled the beekeeping community, and although the phenomenon is far from being solved, there is a relative consensus regarding the detrimental effect the mite <i>Varroa destructor</i> exerts over <i>Apis mellifera</i> populations. In Portugal, this parasite was first recorded in the late 1980s and since then, the beekeepers’ method of choice for controlling the mite are synthetic acaricides, most notably the pyrethroids tau-fluvalinate and flumethrin. Unsurprisingly, resistance to these active ingredients quickly surfaced. Here, we report the first PCR-RFLP resistance assessment in Portuguese apiaries, where 96 <i>V. destructor</i> mites, from different geographic regions, were genotyped between April and August of 2019. The results showed that 47% of the sampled mites exhibit the mutation correlated with resistance. These findings stress the need for a current characterization of the acaricides being used and a description of how the treatments are been applied.
DOI: 10.6084/m9.figshare.24885780
title: Honey bee stocks exhibit high levels of intra-colony variation in viral loads

description: Colonies of the western honey bee, <i>Apis mellifera</i> L., are comprised of tens of thousands of genetically related individuals that can easily share and spread pathogens. Honey bee colonies exhibit variations in disease susceptibility that can translate into highly variable pathogen replication among individuals within a colony, between colonies, and between stocks. Here, we investigate the degree of variation in viral titers of common honey bee viruses (DWV-A, DWV-B, IAPV, LSV2, BQCV) within colonies and between colonies of five honey bee stocks in the United States. Our results showed high intra-colony variation in DWV-A, DWV-B, IAPV, and LSV2, but not in BQCV titers. However, the level of variation was not consistent across stocks. Here, we empirically demonstrate that there is significant intra-colony variation in viral titers and that some stocks are more prone to this variability. Our results highlight the need for further studies comparing virus susceptibility among stocks.
DOI: 10.6084/m9.figshare.24885780.v1
title: Honey bee stocks exhibit high levels of intra-colony variation in viral loads

description: Colonies of the western honey bee, <i>Apis mellifera</i> L., are comprised of tens of thousands of genetically related individuals that can easily share and spread pathogens. Honey bee colonies exhibit variations in disease susceptibility that can translate into highly variable pathogen replication among individuals within a colony, between colonies, and between stocks. Here, we investigate the degree of variation in viral titers of common honey bee viruses (DWV-A, DWV-B, IAPV, LSV2, BQCV) within colonies and between colonies of five honey bee stocks in the United States. Our results showed high intra-colony variation in DWV-A, DWV-B, IAPV, and LSV2, but not in BQCV titers. However, the level of variation was not consistent across stocks. Here, we empirically demonstrate that there is significant intra-colony variation in viral titers and that some stocks are more prone to this variability. Our results highlight the need for further studies comparing virus susceptibility among stocks.
DOI: 10.6084/m9.figshare.19533339
title: Development of monoclonal antibodies against spores of <i>Nosema ceranae</i> for the diagnosis of nosemosis

description: Type C nosemosis caused by the microsporidian fungus <i>Nosema ceranae</i> is a major disease that affects the beekeeping industry worldwide. Nosemosis is very difficult to diagnose as it lacks characteristic symptoms, and any symptoms, presented by a <i>Nosema</i> spp. infections are indistinguishable from those of other diseases seen in honey bees. For these reasons, a novel method was developed for the rapid and accurate diagnosis of nosemosis. Three monoclonal antibodies (mAbs) were developed from three different monoclones (9A4, 14A8, and 19B2) and were found to be reactive to the whole spores during ELISA. The 9A4-, 14A8-, and 19B2-derived mAbs were suitable for western blot analysis and classified as IgG1, IgG2b, and IgG3, respectively. mAbs manufactured in this study have practical applications for nosemosis rapid diagnostic tests. These novel methods, and the production of such kits, may aid the early detection and treatment of nosemosis, help prevent the indiscriminate and needless use of toxic medicines, and prevent hive collapse and monetary losses for beekeepers.
DOI: 10.6084/m9.figshare.19533339.v1
title: Development of monoclonal antibodies against spores of <i>Nosema ceranae</i> for the diagnosis of nosemosis

description: Type C nosemosis caused by the microsporidian fungus <i>Nosema ceranae</i> is a major disease that affects the beekeeping industry worldwide. Nosemosis is very difficult to diagnose as it lacks characteristic symptoms, and any symptoms, presented by a <i>Nosema</i> spp. infections are indistinguishable from those of other diseases seen in honey bees. For these reasons, a novel method was developed for the rapid and accurate diagnosis of nosemosis. Three monoclonal antibodies (mAbs) were developed from three different monoclones (9A4, 14A8, and 19B2) and were found to be reactive to the whole spores during ELISA. The 9A4-, 14A8-, and 19B2-derived mAbs were suitable for western blot analysis and classified as IgG1, IgG2b, and IgG3, respectively. mAbs manufactured in this study have practical applications for nosemosis rapid diagnostic tests. These novel methods, and the production of such kits, may aid the early detection and treatment of nosemosis, help prevent the indiscriminate and needless use of toxic medicines, and prevent hive collapse and monetary losses for beekeepers.
DOI: 10.5519/qd.tpnfho30
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (1 records)
DOI: 10.6084/m9.figshare.16964480
title: Fatty acids and their derivatives from <i>Chlorella vulgaris</i> extracts exhibit <i>in vitro</i> antimicrobial activity against the honey bee pathogen <i>Paenibacillus larvae</i>

description: The green microalga <i>Chlorella vulgaris</i> Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, <i>Chlorella vulgaris</i> has potential as a pollen supplement. We studied whether <i>Chlorella</i> extracts display antimicrobial properties against <i>Paenibacillus larvae</i>, the causative agent of the honey bee bacterial disease American foulbrood. We identified components responsible for antimicrobial activity and evaluated the added values of <i>Chlorella</i> as a food supplement for honey bees. Different extracts (water, acetone, methanol) were prepared from <i>Chlorella</i> biomass (phototrophically and heterotrophically cultivated) and screened for antimicrobial activity against ERIC I and ERIC II genotypes of <i>P. larvae</i>. Active acetone extracts of phototrophically cultivated <i>Chlorella vulgaris</i> biomass were fractioned via preparative reverse-phase chromatography. Antimicrobial activity was detected for 9 of the resulting 33 fractions. Further analysis revealed the chemical composition of the active fractions. <i>C. vulgari</i>s extracts showed a significant antimicrobial effect against vegetative cells and spores of <i>P. larvae</i> strains of ERIC I and ERIC II genotypes. The lowest MIC of the most active acetone extract was 6.3 µg/mL for both tested genotypes. In the majority of the active fractions, monolinolenin, fatty acid linoleic acid, and methyl esters of linoleic and/or palmitic acid were identified via high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis. Based on our results, we concluded that algal <i>C. vulgaris</i> food supplements not only contain nutritional but also potential prophylactic properties for honey bee health.
DOI: 10.6084/m9.figshare.16964480.v2
title: Fatty acids and their derivatives from <i>Chlorella vulgaris</i> extracts exhibit <i>in vitro</i> antimicrobial activity against the honey bee pathogen <i>Paenibacillus larvae</i>

description: The green microalga <i>Chlorella vulgaris</i> Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, <i>Chlorella vulgaris</i> has potential as a pollen supplement. We studied whether <i>Chlorella</i> extracts display antimicrobial properties against <i>Paenibacillus larvae</i>, the causative agent of the honey bee bacterial disease American foulbrood. We identified components responsible for antimicrobial activity and evaluated the added values of <i>Chlorella</i> as a food supplement for honey bees. Different extracts (water, acetone, methanol) were prepared from <i>Chlorella</i> biomass (phototrophically and heterotrophically cultivated) and screened for antimicrobial activity against ERIC I and ERIC II genotypes of <i>P. larvae</i>. Active acetone extracts of phototrophically cultivated <i>Chlorella vulgaris</i> biomass were fractioned via preparative reverse-phase chromatography. Antimicrobial activity was detected for 9 of the resulting 33 fractions. Further analysis revealed the chemical composition of the active fractions. <i>C. vulgari</i>s extracts showed a significant antimicrobial effect against vegetative cells and spores of <i>P. larvae</i> strains of ERIC I and ERIC II genotypes. The lowest MIC of the most active acetone extract was 6.3 µg/mL for both tested genotypes. In the majority of the active fractions, monolinolenin, fatty acid linoleic acid, and methyl esters of linoleic and/or palmitic acid were identified via high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis. Based on our results, we concluded that algal <i>C. vulgaris</i> food supplements not only contain nutritional but also potential prophylactic properties for honey bee health.
DOI: 10.6084/m9.figshare.25233480
title: Experimental parameters affecting the outcomes of amitraz resistance testing in <i>Varroa destructor</i>

description: Honey bee colony health is impaired by a large variety of biotic and abiotic factors. The severe impacts of <i>Varroa destructor</i> and viruses associated with it on honey bees are undeniable and universal. Beekeepers often utilize miticides to mitigate <i>V. destructor</i> populations, but resistance to many widely used synthetic miticides such as amitraz has developed. The Apivar® resistance test has proven to be a valuable tool to monitor for amitraz resistance in <i>V. destructor</i>. However, a more thorough understanding of the effects that changes in testing parameters have on the outcome of the Apivar® resistance is critical for an accurate and comparable interpretation of the results. In this project, the effects of temperature, product age, product exposure surface area, and container reuse on the Apivar® resistance test were studied. High temperature significantly increased control mortality while low temperatures significantly increased Apivar® resistance. The increased Apivar® resistance at low temperature was due to reduced amitraz toxicity at low temperature. There was no association of Apivar® age or manufacturing batch on the outcome of the Apivar® resistance test. The maintenance of Apivar® efficacy with age is likely because the amitraz breakdown product <i>N</i>‘-(2,4-dimethylphenyl)-<i>N</i>-methylformamidine (DPMF) is as toxic as amitraz. The rate and efficacy of the Apivar® resistance test were higher with a larger surface area of Apivar® strip. Reused testing containers or Apivar® strips from colony application yielded reduced efficacy and consistency. This research shows the standard conditions needed to produce comparable data from the Apivar® resistance test. Implications for amitraz resistance monitoring are discussed.
DOI: 10.6084/m9.figshare.25233480.v1
title: Experimental parameters affecting the outcomes of amitraz resistance testing in <i>Varroa destructor</i>

description: Honey bee colony health is impaired by a large variety of biotic and abiotic factors. The severe impacts of <i>Varroa destructor</i> and viruses associated with it on honey bees are undeniable and universal. Beekeepers often utilize miticides to mitigate <i>V. destructor</i> populations, but resistance to many widely used synthetic miticides such as amitraz has developed. The Apivar® resistance test has proven to be a valuable tool to monitor for amitraz resistance in <i>V. destructor</i>. However, a more thorough understanding of the effects that changes in testing parameters have on the outcome of the Apivar® resistance is critical for an accurate and comparable interpretation of the results. In this project, the effects of temperature, product age, product exposure surface area, and container reuse on the Apivar® resistance test were studied. High temperature significantly increased control mortality while low temperatures significantly increased Apivar® resistance. The increased Apivar® resistance at low temperature was due to reduced amitraz toxicity at low temperature. There was no association of Apivar® age or manufacturing batch on the outcome of the Apivar® resistance test. The maintenance of Apivar® efficacy with age is likely because the amitraz breakdown product <i>N</i>‘-(2,4-dimethylphenyl)-<i>N</i>-methylformamidine (DPMF) is as toxic as amitraz. The rate and efficacy of the Apivar® resistance test were higher with a larger surface area of Apivar® strip. Reused testing containers or Apivar® strips from colony application yielded reduced efficacy and consistency. This research shows the standard conditions needed to produce comparable data from the Apivar® resistance test. Implications for amitraz resistance monitoring are discussed.
DOI: 10.6084/m9.figshare.16964480.v1
title: Fatty acids and their derivatives from <i>Chlorella vulgaris</i> extracts exhibit <i>in vitro</i> antimicrobial activity against the honey bee pathogen <i>Paenibacillus larvae</i>

description: The green microalga <i>Chlorella vulgaris</i> Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, <i>Chlorella vulgaris</i> has potential as a pollen supplement. We studied whether <i>Chlorella</i> extracts display antimicrobial properties against <i>Paenibacillus larvae</i>, the causative agent of the honey bee bacterial disease American foulbrood. We identified components responsible for antimicrobial activity and evaluated the added values of <i>Chlorella</i> as a food supplement for honey bees. Different extracts (water, acetone, methanol) were prepared from <i>Chlorella</i> biomass (phototrophically and heterotrophically cultivated) and screened for antimicrobial activity against ERIC I and ERIC II genotypes of <i>P. larvae</i>. Active acetone extracts of phototrophically cultivated <i>Chlorella vulgaris</i> biomass were fractioned via preparative reverse-phase chromatography. Antimicrobial activity was detected for 9 of the resulting 33 fractions. Further analysis revealed the chemical composition of the active fractions. <i>C. vulgari</i>s extracts showed a significant antimicrobial effect against vegetative cells and spores of <i>P. larvae</i> strains of ERIC I and ERIC II genotypes. The lowest MIC of the most active acetone extract was 6.3 µg/mL for both tested genotypes. In the majority of the active fractions, monolinolenin, fatty acid linoleic acid, and methyl esters of linoleic and/or palmitic acid were identified via high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis. Based on our results, we concluded that algal <i>C. vulgaris</i> food supplements not only contain nutritional but also potential prophylactic properties for honey bee health.
DOI: 10.15468/dl.au2b74
title: Occurrence Download

description: A dataset containing 1461053 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Spain",
 "Geometry POLYGON((-5.19732 36.21717,-3.71056 36.70633,-3.68052 36.83866,-3.77814 37.06668,-3.83821 37.31791,-4.13106 37.47897,-4.55907 37.63969,-5.17479 37.63374,-5.58778 37.24621,-5.67789 36.51345,-5.19732 36.21717))",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Plantae"
 ]
}

 The dataset includes 1461053 records from 165 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090753-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.jb24p5
title: Occurrence Download

description: A dataset containing 809054 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rutaceae"
 ]
}

 The dataset includes 809054 records from 2243 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0090619-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.wjhfcs
title: Occurrence Download

description: A dataset containing 7752 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Sciurus niger Linnaeus, 1758"
 ]
}

 The dataset includes 7752 records from 81 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002161-150330110031120/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.jfr9qk
title: Occurrence Download

description: A dataset containing 650 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Protozoa",
 "DecimalLatitude is greater than 40.4704589653052",
 "DecimalLatitude is less than 41.006717716958",
 "DecimalLongitude is greater than -74.3413189800143",
 "DecimalLongitude is less than -73.6363627970185"
 ]
}

 The dataset includes 650 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007433-180824113759888/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.cfm694
title: Occurrence Download

description: A dataset listing the 3288680 species recorded in GBIF matching the query:
{ }

 The dataset's 3288680 records were derived from 31227 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024729-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.17188/1719826
title: Materials Data on V2O5 by Materials Project

description: Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations
DOI: 10.17188/1719827
title: Materials Data on Li2Cu(WO4)2 by Materials Project

description: Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations