DOI: 10.5061/dryad.ht76hdrrq
title: Grapevine cv. tempranillo grafted onto 110R and SO4 rootstocks —gas exchange parameters

description: Severe water stress can lead to hydraulic disfunction, reducing plant
 conductance or even causing death. Some plants exhibit hydraulic
 vulnerability segmentation between organs to reduce this risk. However,
 its role in influencing drought tolerance and resistance in grafted
 plants, such as grapevine, remains unclear. This study aimed to evaluate
 the physiological responses, drought tolerance, hydraulic vulnerability
 segmentation and xylem anatomy of two-year-old Vitis vinifera cv.
 Tempranillo scion grafted onto two rootstocks: 110-Richter (110R) and
 Sélection Oppenheim 4 (SO4). After subjecting the plants to drought
 conditions until the onset of embolism in the leaf (P12), we analysed the
 physiological consequences during recovery. Grapevine exhibits hydraulic
 vulnerability segmentation not only within scion organs but also between
 the scion and rootstock. Although no differences in scion drought
 tolerance and embolism resistance were observed between combinations,
 Tempranillo-110R exhibited higher leaf minimum conductance, leaf P12
 values and root biomass. In contrast, Tempranillo-SO4 displayed larger
 vessel diameter and higher hydraulic conductance. These differences may
 explain the slower recovery of Tempranillo-110R compared to
 Tempranillo-SO4, which showed higher stomatal and root-to-stem hydraulic
 conductance. These findings suggest that rootstock selection should
 consider drought resilience alongside vigour and productivity, especially
 given the increasing the concurrence of severe drought periods due to
 climate change.
description: # Field Data. Gas exchange parameters
 [https://doi.org/10.5061/dryad.ht76hdrrq](https://doi.org/10.5061/dryad.ht76hdrrq) ## Description of the data and file structure In the second group of plants (n=6), vine water status and leaf gas exchange measurements were conducted in the plants maintained under field conditions at WW, WD, R1 and R2 stages. Predawn leaf water potential (ΨPD) and midday stem water potential (Ψstem) were measured using a pressure chamber (Model 600, PMS Instruments, USA) in fully expanded leaves. ΨPD measurements were taken before sunrise, between 05:00 and 06:00, while Ψstem measurements were conducted at solar noon on leaves that had been covered for 1 h inside a zip-lock bag with metallized high-density polyethylene reflective film (Sonoco RF, Sonoco Products Co., USA), to halt transpiration and allow the Ψleaf to equilibrate with the stem potential. Gas exchange measurements, including leaf AN, gs, and transpiration (E) were performed on sunny days in the mid-morning (09:00-11:00) using a Li-6400 gas exchange analyser (Li-cor Inc., USA) equipped with an open-top chamber in similar leaves than the ones used for Ψstem determination. From these measurements, intrinsic water use efficiency (WUEi) (Flexas *et al*., 2010) and hydraulic conductance from root to stem (Kroot-stem) (Tsuda and Tyree, 2000) were calculated. Briefly, it consists in calculating the hydraulic conductance between the root and the stem as the quotient of the maximum leaf transpiration (E) rate and the difference in water potential between the soil, assumed to be in equilibrium with pre-dawn water potential (ΨPD), and the stem (Ψstem) at noon. ### Files and variables #### File: Field\_Data\_.xlsx **Description:**  ##### Variables * Photsynthesis (Photo) * Stomatal conductance (Cond) * Water Use Efficiency (WUE) * Leaf transpiration (Trmmol) * Number of leaves (Nº leaves) * Tasa aparición hojas (Leaf growth rate) * Predawn leaf water potential (Ypredawn) * Stem water potential (Ystem) * Root-to-stem hydraulic conductance (Kroot-stem) * Plan hydraulic conductance (Kplant) ## Code/software Microsof Excel is the only software needed to view this file.