DOI: 10.22002/0gck3-4k396
title: NHP2L1 siRNA virus particles

description: HIV particles from producer cells treated with NHP2L1 siRNA
description: <strong>Tilt Series Date:</strong> 2012-09-17</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Gaelle Mercenne (Sundquist lab) generated virus. Viral particles were shipped to Caltech to be frozen and imaged by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Virus made in presence of NHP2L1 siRNA</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-09-17-4/3dimage_69449/</p>12sep17_c141_2_AT1BT1_0004_BIN_full.rec,  Reconstruction (Pixel Size 2.0 nm), 427.5 MB  
        
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        12sep17_c141_2_AT1BT1_0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
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        c141_2_0004_particle_4.tiff,  file associated to 3D Image #69449, 983.3 kB  
        
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        c141_2_0004_particle_4_topslice.tiff,  file associated to 3D Image #69449, 777.2 kB  
        
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        c141_2_0004_particle_4_membrane_a.tiff,  file associated to 3D Image #69449, 788.9 kB  
        
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        c141_2_0004_particle_4_intenaldens.tiff,  file associated to 3D Image #69449, 1.8 MB  
        
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        c141_2_0004_particle_2.tiff,  file associated to 3D Image #69449, 974.5 kB  
        
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        c141_2_0004_particle_2-3.tiff,  file associated to 3D Image #69449, 987.8 kB  
        
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        c141_2_0004_particle_1.tiff,  file associated to 3D Image #69449, 986.6 kB  
        
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        12sep17_c141_2_AT1BT1_0004_BIN_full.tiff,  file associated to 3D Image #69449, 889.1 kB  
        
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        c141_2_004_NHP2L1.mov,  file associated to 3D Image #69449, 3.2 MB  
        
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