DOI: 10.5061/dryad.h9w0vt4x2
title: Data from: A lateralized pathway for associating nutrients with flavors

description: This dataset contains the data underlying the main figures presented in
 Grove et al., 2025. Data are provided as Microsoft Excel (.xlsx) files,
 where each file corresponds to a primary figure in the manuscript, and
 individual worksheets represent the data used to generate each subfigure
 (e.g. Fig. 1a). Submission of this dataset is intended to ensure
 transparency and reproducibility of the results, and the data herein made
 be reused for secondary analysis, provided citation.
description: # Data from: A lateralized pathway for associating nutrients with flavors
 Dataset DOI:
 [10.5061/dryad.h9w0vt4x2](https://doi.org/10.5061/dryad.h9w0vt4x2) ##
 Description of the data and file structure This readme file provides
 instructions on reproducing the results presented in the research article
 titled " A lateralized pathway for associating nutrients with
 flavors," published by Grove et al. The article can be accessed at
 [https://www.biorxiv.org/content/10.1101/2025.02.09.637334v2](https://www.biorxiv.org/content/10.1101/2025.02.09.637334v2). We have submitted our raw data for main figure 1 (**figure1.xls**), main figure 2 (**figure2.xls **), main figure 3 (**figure3.xls **), main figure 4 (**figure4.xls **), main figure 5 (**figure5.xls**), and main figure 6 (**figure6.xls **). ### Files and variables **Figure 1 Data - figure1.xlsx** \* Figure 1a -  WT flavor conditioning \* Preference for the CS+: percentage of licks corresponding the CS+ in a two-bottle test between the CS+ and CS- before and after training.  \* Figure 1b - control_fc \* Preference for the CS+: percentage of licks corresponding the CS+ in a two-bottle test between the CS+ and CS- before and after training for control animals (no GtACR). \* Figure 1b - gtacr_fc \* Preference for the CS+: percentage of licks corresponding the CS+ in a two-bottle test between the CS+ and CS- before and after training for GtACR expressing animals. \* Figure 1c \_cs1 \* Licks of the CS-: Licks of the CS- during day 1 of training for GtACR and control animals  \* Figure 1c \_ns1 \* Licks of the CS+: Licks of the CS+ during day 1 of training for GtACR and control animals  \* Figure 1d \_control \* Licks of Ensure: Licks of Ensure for control anmals (no GtACR) with (stim) or without (no stim) concurrent laser stimulation.    \* Figure 1d \_GtACR \* Licks of Ensure: Licks of Ensure for GtACR-expressing anmals with (stim) or without (no stim) concurrent laser stimulation.    \* Figure 1e \_CTA \* Sucrose preference: Percentage of licks for sucrose in a two-bottle test between sucrose and water after CTA training. Data is for animals that express GtACR (GtACR) or do not express GtACR (control). All animals received laser during training. **Figure 2 Data - figure2.xlsx** \* Figure 2a_ds_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in dorsal striatum (DS) during IG Ensure infusion (0-60 min; infusion from 10-20 min).  \* Figure 2a_nac_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in nucleus accumbens (nac) during IG Ensure infusion (0-60 min; infusion from 10-20 min).  \* Figure 2a_mbla_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in medial basolateral amygdala (mbla) during IG Ensure infusion (0-60 min; infusion from 10-20 min).  \* Figure 2a_NAC_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in nucleus accumbens (nac) during the 10 min IG Ensure infusion.  \* Figure 2a_lBLA_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in lateral basolateral amygdala (lBLA) during the 10 min IG Ensure infusion.  \* Figure 2a_mBLA_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in medial basolateral amygdala (mBLA) during the 10 min IG Ensure infusion.  \* Figure 2a_DLS_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in dorsolateral striatum (DLS) during the 10 min IG Ensure infusion.  \* Figure 2b_DLS_trace \* Dopamine release measured as z-scored fluorescence using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in dorsal lateral striatum (DLS) during oral Ensure ingestion (-20 to + 20 sec; start of ingestion is time 0).  \* Figure 2b_DLS_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in DLS during the first 5 sec of the first lick bout of Ensure. \* Figure 2b_mBLA_trace \* Dopamine release measured as z-scored fluorescence using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in medial BLA (mBLA) during oral Ensure ingestion (-20 to + 20 sec; start of ingestion is time 0).  \* Figure 2b_mBLA_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in mBLA during the first 5 sec of the first lick bout of Ensure. \* Figure 2b_lBLA_trace \* Dopamine release measured as z-scored fluorescence using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in lateral BLA (mBLA) during oral Ensure ingestion (-20 to + 20 sec; start of ingestion is time 0).  \* Figure 2b_lBLA_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in lBLA during the first 5 sec of the first lick bout of Ensure. \* Figure 2b_NAC_trace \* Dopamine release measured as z-scored fluorescence using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in NAc during oral Ensure ingestion (-20 to + 20 sec; start of ingestion is time 0).  \* Figure 2b_NAc_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in NAc during the first 5 sec of the first lick bout of Ensure. \* Figure 2c_NAc_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in NAc during the first 5 sec after the first lick bout of Ensure. \* Figure 2c_DLS_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in DLS during the first 5 sec after the first lick bout of Ensure. \* Figure 2c_lBLA_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in lBLA during the first 5 sec after the first lick bout of Ensure. \* Figure 2c_mBLA_bar plot \* Dopamine release measured as z-scored GRAB-DA3m fluorescence: Mean GRAB-DA3m fluorescence in mBLA during the first 5 sec after the first lick bout of Ensure. \* Figure 2d_lBLA_bar plot \* Time to peak (sec): Time to peak GRAB-DA3m fluorescence in lBLA during the first 30 sec of the first lick bout of Ensure. \* Figure 2d_mBLA_bar plot \* Time to peak (sec): Time to peak GRAB-DA3m fluorescence in lmBLA during the first 30 sec of the first lick bout of Ensure. \* Figure 2d_NAc_bar plot \* Time to peak (sec): Time to peak GRAB-DA3m fluorescence in NAc during the first 30 sec of the first lick bout of Ensure. \* Figure 2d_DLS_bar plot \* Time to peak (sec): Time to peak GRAB-DA3m fluorescence in DLS during the first 30 sec of the first lick bout of Ensure. \* Figure 2e_aBLA_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in anterior BLA (aBLA) during IG Ensure infusion (0-60 min; infusion from 10-20 min).  \* Figure 2e_pBLA_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in posterior BLA (pBLA) during IG Ensure infusion (0-60 min; infusion from 10-20 min).  \* Figure 2e_aBLA_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in aBLA during the 10 min IG Ensure infusion.  \* Figure 2e_pBLA_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in pBLA during the 10 min IG Ensure infusion.  \* Figure 2f_aBLA_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in aBLA during the first lick bout of Ensure.  \* Figure 2f_pBLA_bar plot \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in pBLA during the first lick bout of Ensure.  **Figure 3 Data  - figure3.xlsx** *Figure 3a_sham_heatmap *Heatmap of z-scored activity of recorded VTADA neurons (y-axis) over time (x) in 4 Hz increments during sham infusion. *Figure 3a_ensure_heatmap *Heatmap of z-scored activity of recorded VTADA neurons (y-axis) over time (x) in 4 Hz increments during Ensure IG infusion (from x=10–20 min). *Figure 3a_ensure_ks *z-scores and probabilities for VTADA neuron activity distributions during ensure IG infusion *Figure 3a_sham_ks *z-scores and probabilities for VTADA neuron activity distributions during sham infusion *Figure 3b_sham_heatmap *Heatmap of z-scored activity of recorded VTADA/CCK neurons (y-axis) over time (x) in 4 Hz increments during sham infusion. *Figure 3b_ensure_heatmap *Heatmap of z-scored activity of recorded VTADA/CCK neurons (y-axis) over time (x) in 4 Hz increments during Ensure IG infusion (from x=10–20 min). *Figure 3b_water_ks *z-scores and probabilities for VTADA/CCK neuron activity distributions during water IG infusion *Figure 3b_ensure_ks *z-scores and probabilities for VTADA/CCK neuron activity distributions during ensure IG infusion *Figure 3b_sham_ks *z-scores and probabilities for VTADA/CCK neuron activity distributions during sham infusion *Figure 3d_silhouette *silhouette scores for each k of k-means clustering for VTADA/CCK neuron responses during ensure IG *Figure 3d_varexplained *variance explained by each PC of VTADA/CCK neuron responses during ensure IG *Figure 3d_cluster1 *mean z-scored activity of k-means cluster 1 of VTADA/CCK neuron responses during ensure IG *Figure 3d_cluster2 *mean z-scored activity of k-means cluster 2 of VTADA/CCK neuron responses during ensure IG *Figure 3e_pc1 *PC1 of VTADA/CCK neuron responses during ensure IG *Figure 3e_pc2 *PC2 of VTADA/CCK neuron responses during ensure IG *Figure 3f_sham_heatmap *Heatmap of z-scored activity of recorded VTADA/LepR neurons (y-axis) over time (x) in 4 Hz increments during sham infusion. *Figure 3f_ensure_heatmap *Heatmap of z-scored activity of recorded VTADA/LepR neurons (y-axis) over time (x) in 4 Hz increments during Ensure IG infusion (from x=10–20 min). *Figure 3g_water_ks *z-scores and probabilities for VTADA/LepR neuron activity distributions during water IG infusion *Figure 3g_ensure_ks *z-scores and probabilities for VTADA/LepR neuron activity distributions during ensure IG infusion *Figure 3g_sham_ks *z-scores and probabilities for VTADA/LepR neuron activity distributions during sham infusion *Figure 3i_mbla *Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in mBLA VGluT neurons during Ensure IG infusion. *Figure 3i_cea *Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in CeA VGAT neurons during Ensure IG infusion. *Figure 3j_mbla \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in mBLA VGluT neurons during the first lick bout of Ensure.  *Figure 3j_cea \* Dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in CeA VGAT neurons during the first lick bout of Ensure.  *Figure 3k_mbla \* Timing of dopamine release peak in mBLA VGluT neurons during the first lick bout of Ensure.  *Figure 3k_cea \* Timing of dopamine release peak in CeA VGAT neurons during the first lick bout of Ensure.  **Figure 4 Data  - figure4.xlsx** \* Figure 4b_aBLA \* CS+ preference: Percentage of licks for the CS+ in a two bottle test (CS+ vs. CS-) performed before and after flavor conditioning for animals that received delayed (20s) closed-loop stimulation in the aBLA.  \* Figure 4b_pBLA \* CS+ preference: Percentage of licks for the CS+ in a two bottle test (CS+ vs. CS-) performed before and after flavor conditioning for animals that received delayed (20s) closed-loop stimulation in the pBLA.  \* Figure 4b_CeA \* CS+ preference: Percentage of licks for the CS+ in a two bottle test (CS+ vs. CS-) performed before and after flavor conditioning for animals that received delayed (20s) closed-loop stimulation in the CeA. \* Figure 4b_NAc \* CS+ preference: Percentage of licks for the CS+ in a two bottle test (CS+ vs. CS-) performed before and after flavor conditioning for animals that received delayed (20s) closed-loop stimulation in the NAc. \* Figure 4b_DLS \* CS+ preference: Percentage of licks for the CS+ in a two bottle test (CS+ vs. CS-) performed before and after flavor conditioning for animals that received delayed (20s) closed-loop stimulation in the DLS. \* Figure 4c_aBLA \* Sucrose preference after CTA: Percentage of licks for the sucrose in a two bottle test (sucrose vs. water) performed after the animals received delayed (20s) closed-loop stimulation in the aBLA. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4c_pBLA \* Sucrose preference after CTA: Percentage of licks for the sucrose in a two bottle test (sucrose vs. water) performed after the animals received delayed (20s) closed-loop stimulation in the pBLA. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4c_CeA \* Sucrose preference after CTA: Percentage of licks for the sucrose in a two bottle test (sucrose vs. water) performed after the animals received delayed (20s) closed-loop stimulation in the CeA. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4c_NAc \* Sucrose preference after CTA: Percentage of licks for the sucrose in a two bottle test (sucrose vs. water) performed after the animals received delayed (20s) closed-loop stimulation in the NAc. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4c_DLS \* Sucrose preference after CTA: Percentage of licks for the sucrose in a two bottle test (sucrose vs. water) performed after the animals received delayed (20s) closed-loop stimulation in the DLS. Data is shown for animals that express ChR2 and controls that do not (mCherry).  * Figure 4d_aBLA \* Lever presses in self-stimulation: Difference between active and inactive lever presses in a self-stimulation experiment in which mice had optical fibers in the aBLA. Data is shown for animals that express ChR2 and controls that do not (mCherry).  * Figure 4d_pBLA \* Lever presses in self-stimulation: Difference between active and inactive lever presses in a self-stimulation experiment in which mice had optical fibers in the pBLA. Data is shown for animals that express ChR2 and controls that do not (mCherry).  * Figure 4d_CeA \* Lever presses in self-stimulation: Difference between active and inactive lever presses in a self-stimulation experiment in which mice had optical fibers in the CeA. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4d_NAc \* Lever presses in self-stimulation: Difference between active and inactive lever presses in a self-stimulation experiment in which mice had optical fibers in the NAc. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4d_DLS \* Lever presses in self-stimulation: Difference between active and inactive lever presses in a self-stimulation experiment in which mice had optical fibers in the DLS. Data is shown for animals that express ChR2 and controls that do not (mCherry). \* Figure 4e_aBLA \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick ON) for mice that had optical fibers in the aBLA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4e_pBLA \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick ON) for mice that had optical fibers in the pBLA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4e_CeA \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick ON) for mice that had optical fibers in the CeA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4e_NAc \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick ON) for mice that had optical fibers in the NAc. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4e_DLS \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick ON) for mice that had optical fibers in the DLS. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4f_aBLA \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick OFF) for mice that had optical fibers in the aBLA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4f_pBLA \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick OFF) for mice that had optical fibers in the pBLA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4f_CeA \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick OFF) for mice that had optical fibers in the CeA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4f_NAc \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick OFF) for mice that had optical fibers in the NAc. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4f_DLS \* Licks during closed-loop stimulation: Total licks of Ensure during closed-loop stimulation (lick OFF) for mice that had optical fibers in the DLS. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4g_aBLA \* Licks during continuous stimulation: Total licks of Ensure during continuous stimulation for mice that had optical fibers in the aBLA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4g_pBLA \* Licks during continuous stimulation: Total licks of Ensure during continuous stimulation for mice that had optical fibers in the pBLA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4g_CeA \* Licks during continuous stimulation: Total licks of Ensure during continuous stimulation for mice that had optical fibers in the CeA. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4g_NAc \* Licks during continuous stimulation: Total licks of Ensure during continuous stimulation for mice that had optical fibers in the NAc. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  \* Figure 4g_DLS \* Licks during continuous stimulation: Total licks of Ensure during continuous stimulation for mice that had optical fibers in the DLS. Data is shown for experiments where animals received laser stimulation (stim) and experiments where they did not (control).  **Figure 5 Data - figure5.xlsx** *Figure 5c_auroc_heatmap \* auROC of activity during sucrose lick bout (compared to simulated lick bout) in aBLA-D1R neurons (y-axis) by time (x-axis) in 4Hz increments. *Figure 5c_sucrose_trace *z-scores and probabilities for aBLA-D1R neuron activity distributions during sucrose lick bouts *Figure 5c_mock_trace *z-scores and probabilities for aBLA-D1R neuron activity distributions during simulated lick bouts \* Figure 5e_data \* Mean z-score: Mean z-score for calcium response of individual neurons during bout of sucrose or sucralose ingestion.  *Figure 5f_sucrose_heatmap *z-scored activity during sucrose lick bouts in aBLA-D1R neurons (y-axis) by time (x-axis) in 4Hz increments  *Figure 5f_water_heatmap *z-scored activity during water lick bouts in aBLA-D1R neurons (y-axis) by time (x-axis) in 4Hz increments  *Figure 5f_sucralose_heatmap *z-scored activity during sucralose lick bouts in aBLA-D1R neurons (y-axis) by time (x-axis) in 4Hz increments  *Figure 5f_sucrose_pcspace *PC loading during sucrose lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5f_sucralose_pcspace *PC loading during sucralose lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5f_water_pcspace *PC loading during water lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5f_mock_pcspace *PC loading during simulated lick bouts, where PCx loading at time y is represented as pcx_y. \* Figure 5g_sucrose_trace \* Mean z-score: Time (min) versus mean z-score for calcium response in response to IG infusion of sucrose. \* Figure 5g_sham_trace \* Mean z-score: Time (min) versus mean z-score for calcium response in response to sham IG infusion. \* Figure 5g_data \* Mean z-score: Mean z-score for calcium response of individual neurons during IG infusion of sham, sucrose, Ensure, or Intralipid. *Figure 5h_sucralose_first_trace *PC loading during early sucralose lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5h_sucralose_last_trace *PC loading during late sucralose lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5h_sucrose_first_trace *PC loading during early sucrose lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5h_sucrose_last_trace *PC loading during late sucrose lick bouts, where PCx loading at time y is represented as pcx_y. *Figure 5h_distances *Euclidian distances in PC space between early and late bouts, over time in 4 Hz increments. \* Figure 5j_sucrose \* Mean z-score: Mean z-score for calcium response of individual neurons during either the first 5 or last 5 bouts of oral ingestion of sucrose. \* Figure 5j_sucralose \* Mean z-score: Mean z-score for calcium response of individual neurons during either the first 5 or last 5 bouts of oral ingestion of sucralose. \* Figure 5l_control \* Preference for the CS+: percentage of licks corresponding the CS+ in a two-bottle test between the CS+ and CS- before and after training for control animals (no GtACR). \* Figure 5l_GtACR \* Preference for the CS+: percentage of licks corresponding the CS+ in a two-bottle test between the CS+ and CS- before and after training for GtACR expressing animals. **Figure 6 Data - figure6.xlsx** \* Figure 6a_left_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in left BLA during IG Ensure infusion (0-60 min; infusion is from min 10-20).  \* Figure 6a_right_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in right BLA during IG Ensure infusion (0-60 min; infusion is from min 10-20).  \* Figure 6a_barplot \* Mean dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in left or right BLA for individual mice during IG Ensure infusion (infusion is from min 10-20).  \* Figure 6b_barplot \* Mean dopamine release measured as delta F/F using GRAB-DA3m: Mean GRAB-DA3m fluorescence in left or right BLA for individual mice during the first lick bout of oral Ensure consumption.  \* Figure 6d_right \* Dopamine release measured as delta F/F using GRAB-DA3m: Representative trace of time course of GRAB-DA3m fluorescence in right BLA at rest (4 minutes shown).  \* Figure 6d_left \* Dopamine release measured as delta F/F using GRAB-DA3m: Representative trace of time course of GRAB-DA3m fluorescence in left BLA at rest (4 minutes shown). Recording is simultaneous with data above from right BLA in the same mouse. \* Figure 6h_leftbla_rightvgx_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in left BLA in response to IG Ensure infusion (0-60 min; infusion is from min 10-20) in mice that received a right vagotomy.  \* Figure 6h_rightbla_rightvgx_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in right BLA in response to IG Ensure infusion (0-60 min; infusion is from min 10-20) in mice that received a right vagotomy.  \* Figure 6h_leftbla_leftvgx_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in left BLA in response to IG Ensure infusion (0-60 min; infusion is from min 10-20) in mice that received a left vagotomy.  \* Figure 6h_rightbla_leftvgx_trace \* Dopamine release measured as delta F/F using GRAB-DA3m: Time course of mean GRAB-DA3m fluorescence in right BLA in response to IG Ensure infusion (0-60 min; infusion is from min 10-20) in mice that received a left vagotomy.  *Figure 6i_rightvgx *Left - right BLA dopamine release as delta F/F using GRAB-DA3m: Average GRAB-DA3m fluorescence in left BLA subtracted by fluorescence in right BLA before and after right vagotomy. *Figure 6i_leftvgx *Left - right BLA dopamine release as delta F/F using GRAB-DA3m: Average GRAB-DA3m fluorescence in left BLA subtracted by fluorescence in right BLA before and after left vagotomy. *Figure 6l_data *Labeling loads in mice where PRV-Introvert was used to anterogradely label VTA-DA neurons projecting to left (8 mice) or right (6 mice) BLA, and upstream neurons. Loads are normalized by load in VTA. *Figure 6m_data *Connectivity matrix between top labelled brain regions. ## Code/software This data was analyzed using MATLAB v2023, but can be viewed and analyzed using MATLAB, R, Prism, or any other major data analysis software/code. ## Access information The data was collected in a research laboratory (Knight Lab at UCSF): [https://knightlab.ucsf.edu/](https://knightlab.ucsf.edu/). The graphical displays of this source data can be found at [https://www.biorxiv.org/content/10.1101/2025.02.09.637334v2](https://www.biorxiv.org/content/10.1101/2025.02.09.637334v2)