cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6992093
title: Mild reproductive effects of the Tityus bahiensis scorpion venom in rats

description: BackgroundScorpion envenoming is a public health problem in Brazil, whereTityus serrulatus and T. bahiensisare considered the most dangerous scorpions. They are well adapted to urbanized environments, and there is an increasing probability of human exposure to these venoms, including during pregnancy. Not much is known about the effects of prenatal exposure to the venom, and no information is available to aid in the rational treatment of victims stung during pregnancy. Thus, this study aimed to investigate whether venom from the scorpion T. bahiensis administered once to pregnant female rats at a dose that causes a moderate envenomation may lead to deleterious effects on the reproductive performance of the dams and on the development of their offspring. This is the first work demonstrating that T. bahiensis venom, when administered experimentally to rats, alters maternal reproductive performance and the morphological development of fetuses. The venom was given to dams on the 5th (GD5) or on the 10th (GD10) gestational day. After laparotomy, on GD21, fetuses and placentas were counted, weighed and externally analyzed. The corpora lutea were counted. The sex and vitality of fetuses were evaluated, and each litter was then randomly divided for visceral or skeletal analyses. Data were analyzed by ANOVA followed by the Tukey-Kramer test and Fisher's exact test. The significance level for all tests was set at p &lt; 0.05.Results: GD5 group presented an increased number of pre-implantation losses. Weight gains in fetuses and placentas were observed in the GD5 and GD10 groups. Weights of the heart and lungs were elevated in GD5 and GD10 and liver weight in GD10.Conclusions: Moderate envenomation by T. bahiensis scorpion venom alters maternal reproductive performance and fetal development. However, these are preliminary results whose causes should be investigated more carefully in future studies.
DOI: 10.6084/m9.figshare.6992093.v1
title: Mild reproductive effects of the Tityus bahiensis scorpion venom in rats

description: BackgroundScorpion envenoming is a public health problem in Brazil, whereTityus serrulatus and T. bahiensisare considered the most dangerous scorpions. They are well adapted to urbanized environments, and there is an increasing probability of human exposure to these venoms, including during pregnancy. Not much is known about the effects of prenatal exposure to the venom, and no information is available to aid in the rational treatment of victims stung during pregnancy. Thus, this study aimed to investigate whether venom from the scorpion T. bahiensis administered once to pregnant female rats at a dose that causes a moderate envenomation may lead to deleterious effects on the reproductive performance of the dams and on the development of their offspring. This is the first work demonstrating that T. bahiensis venom, when administered experimentally to rats, alters maternal reproductive performance and the morphological development of fetuses. The venom was given to dams on the 5th (GD5) or on the 10th (GD10) gestational day. After laparotomy, on GD21, fetuses and placentas were counted, weighed and externally analyzed. The corpora lutea were counted. The sex and vitality of fetuses were evaluated, and each litter was then randomly divided for visceral or skeletal analyses. Data were analyzed by ANOVA followed by the Tukey-Kramer test and Fisher's exact test. The significance level for all tests was set at p &lt; 0.05.Results: GD5 group presented an increased number of pre-implantation losses. Weight gains in fetuses and placentas were observed in the GD5 and GD10 groups. Weights of the heart and lungs were elevated in GD5 and GD10 and liver weight in GD10.Conclusions: Moderate envenomation by T. bahiensis scorpion venom alters maternal reproductive performance and fetal development. However, these are preliminary results whose causes should be investigated more carefully in future studies.
DOI: 10.6084/m9.figshare.6992087
title: Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected withHelicobacter pylori

description: Background : Epidemiological investigations have indicated thatHelicobacter pyloriinduces inflammation in the gastric mucosa regulated by several interleukins. The genesIL1BandIL8are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits inH. pyloriinfected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection ofH. pyloriand analysis of the genes mentioned above. Patients with gastritis had a higher frequency ofH. pylori-positive samples.Results : H. pyloriwas detected in 30/60 patients (50%) by PCR. As for polymorphism of interleukin 8 (-251) gene we observed a statistical difference when analyzed TA (p= 0.039) and TT (p= 0.047) genotypes. In theIL1B31 there was a statistical difference in TT (p= 0.01) genotype and in theIL1B-511there wasn’t any statistical difference.Conclusion : Our results suggest a strong correlation between the presence of chronic gastritis and infection byH. pyloriand thatIL1B-31TTandIL8-251TTgenotypes appear to act as protective factors againstH. pyloriinfection whileIL8-251TAgenotype may comprise a risk factor for infection with this bacterium.
DOI: 10.6084/m9.figshare.6992081
title: Marker-assisted pyramiding of resistance loci to grape downy mildew

description: Abstract: The objective of this work was to use a marker-assisted selection for pyramiding the resistance loci Rpv1 and Rpv3.1 in grapevine (Vitis vinifera), and to evaluate their conferred resistance against Brazilian downy mildew (Plasmopara viticola) isolates. A progeny of 23 plants, segregating for the two resistance loci, was obtained by the cross between the Gf 2000-305-122 and Gf.Ga-52-42 genotypes. The progeny was genotyped with four microsatellite markers and phenotyped for resistance to P. viticola using a bioassay with leaf discs. Six plants containing the Rpv1 and Rpv3.1 pyramided loci were identified by the molecular analysis. Plants harboring the Rpv1 + Rpv3.1, Rpv3.1, and Rpv1 loci showed 12.8, 30.0, and 33.1 sporangiophores per leaf disc, respectively. Plants with no resistance loci showed a dense sporulation. The phenotypic analysis of the expression of the two pyramided loci was only confirmed for four plants that showed the highest resistance level, i.e., mean value of 1.8 sporangiophores. A high-throughput method for pyramiding the Rpv1 and Rpv3.1 loci was developed, which confirmed the increased resistance to P. viticola. The selected elite genetic material shows a high resistance to downy mildew and elevated enological potential for grapevine breeding in Brazil.
DOI: 10.6084/m9.figshare.6992087.v1
title: Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected withHelicobacter pylori

description: Background : Epidemiological investigations have indicated thatHelicobacter pyloriinduces inflammation in the gastric mucosa regulated by several interleukins. The genesIL1BandIL8are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits inH. pyloriinfected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection ofH. pyloriand analysis of the genes mentioned above. Patients with gastritis had a higher frequency ofH. pylori-positive samples.Results : H. pyloriwas detected in 30/60 patients (50%) by PCR. As for polymorphism of interleukin 8 (-251) gene we observed a statistical difference when analyzed TA (p= 0.039) and TT (p= 0.047) genotypes. In theIL1B31 there was a statistical difference in TT (p= 0.01) genotype and in theIL1B-511there wasn’t any statistical difference.Conclusion : Our results suggest a strong correlation between the presence of chronic gastritis and infection byH. pyloriand thatIL1B-31TTandIL8-251TTgenotypes appear to act as protective factors againstH. pyloriinfection whileIL8-251TAgenotype may comprise a risk factor for infection with this bacterium.
DOI: 10.6084/m9.figshare.6992081.v1
title: Marker-assisted pyramiding of resistance loci to grape downy mildew

description: Abstract: The objective of this work was to use a marker-assisted selection for pyramiding the resistance loci Rpv1 and Rpv3.1 in grapevine (Vitis vinifera), and to evaluate their conferred resistance against Brazilian downy mildew (Plasmopara viticola) isolates. A progeny of 23 plants, segregating for the two resistance loci, was obtained by the cross between the Gf 2000-305-122 and Gf.Ga-52-42 genotypes. The progeny was genotyped with four microsatellite markers and phenotyped for resistance to P. viticola using a bioassay with leaf discs. Six plants containing the Rpv1 and Rpv3.1 pyramided loci were identified by the molecular analysis. Plants harboring the Rpv1 + Rpv3.1, Rpv3.1, and Rpv1 loci showed 12.8, 30.0, and 33.1 sporangiophores per leaf disc, respectively. Plants with no resistance loci showed a dense sporulation. The phenotypic analysis of the expression of the two pyramided loci was only confirmed for four plants that showed the highest resistance level, i.e., mean value of 1.8 sporangiophores. A high-throughput method for pyramiding the Rpv1 and Rpv3.1 loci was developed, which confirmed the increased resistance to P. viticola. The selected elite genetic material shows a high resistance to downy mildew and elevated enological potential for grapevine breeding in Brazil.
DOI: 10.6084/m9.figshare.6992078
title: Detection of antibodies to Toxoplasma gondii in wild animals in Brazil

description: Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10 (4; 66.7%) and 40 (2; 33.3%). The species Pseudalopex vetulus,Cerdocyon thous, Hydrochoerus hydrochaeris and Tapyrus terrestris had titers of 10, while Alouatta caraya and Puma concolor had titers of 40. There was no significant association regarding age, gender or purpose of care (p&gt; 0.05).Conclusions Carnivorous, herbivorous and omnivorous wild animals are potential sentinels of human toxoplasmosis, especially when wild felids are present, maintaining the environmental contamination.
DOI: 10.6084/m9.figshare.6992078.v1
title: Detection of antibodies to Toxoplasma gondii in wild animals in Brazil

description: Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10 (4; 66.7%) and 40 (2; 33.3%). The species Pseudalopex vetulus,Cerdocyon thous, Hydrochoerus hydrochaeris and Tapyrus terrestris had titers of 10, while Alouatta caraya and Puma concolor had titers of 40. There was no significant association regarding age, gender or purpose of care (p&gt; 0.05).Conclusions Carnivorous, herbivorous and omnivorous wild animals are potential sentinels of human toxoplasmosis, especially when wild felids are present, maintaining the environmental contamination.
DOI: 10.6084/m9.figshare.6992072
title: The Chemical Composition of Winter Fogs at São Paulo Highway Sites

description: Radiation and advection fogs were characterized at two sites nearby important roads next to São Paulo City, Brazil. Water-soluble ions, monosaccharides, other sugars and organic and elemental carbon (OC and EC) were determined. Both sites present a high number of fog events with mainly radiation fogs causing visibility problems to the automotive traffic (Castello Branco and Raposo Tavares roads). Samples were collected in nine events in 2009 and ten events in 2010, during wintertime. Two radiation and advection fog events have been herein presented. The radiation fog occurred in 2009 at Castello Branco Road (CBR), presented the highest concentration of pollutants comparing to all other events. The most abundant species are associated with biomass landfill and fossil fuel burning. Locally, the region has a strong impact by the fertilizer industries and traffic of heavy vehicles. The second most polluted event occurred also in 2009 at CBR, an advection fog presented species attributed to soil resuspension and anthropogenic emissions. The results are compared with rainwater composition studies.
DOI: 10.6084/m9.figshare.6992072.v1
title: The Chemical Composition of Winter Fogs at São Paulo Highway Sites

description: Radiation and advection fogs were characterized at two sites nearby important roads next to São Paulo City, Brazil. Water-soluble ions, monosaccharides, other sugars and organic and elemental carbon (OC and EC) were determined. Both sites present a high number of fog events with mainly radiation fogs causing visibility problems to the automotive traffic (Castello Branco and Raposo Tavares roads). Samples were collected in nine events in 2009 and ten events in 2010, during wintertime. Two radiation and advection fog events have been herein presented. The radiation fog occurred in 2009 at Castello Branco Road (CBR), presented the highest concentration of pollutants comparing to all other events. The most abundant species are associated with biomass landfill and fossil fuel burning. Locally, the region has a strong impact by the fertilizer industries and traffic of heavy vehicles. The second most polluted event occurred also in 2009 at CBR, an advection fog presented species attributed to soil resuspension and anthropogenic emissions. The results are compared with rainwater composition studies.
DOI: 10.6084/m9.figshare.6992063
title: A model-based Decision Support System for multiple container terminals hub management

description: Abstract Paper aims To develop a model-based Decision Support System (DSS) that allows identifying the best strategy of the inter-/intra-terminal flows of the containers in order to increasing the performance of the hub under economic and environmental perspective. Originality The adoption of a dry port can effectively solve the congestion problem of a terminal only if an integrated sustainable solution (dry port location and container strategy storage) is identified. Research method The model is based on a heuristic computational algorithm for non-linear programming. Main findings The application of DSS to a full-scale numerical case show the model capabilities in identifying the optimal logistic strategies ensuring a low CF and in optimizing the cost due to transport activities. Implications for theory and practice It is possible to identify different strategies allowing to obtain an eco-friendly solution reducing, at same time, the costs for a given number of containers to be handled.
DOI: 10.6084/m9.figshare.6992063.v1
title: A model-based Decision Support System for multiple container terminals hub management

description: Abstract Paper aims To develop a model-based Decision Support System (DSS) that allows identifying the best strategy of the inter-/intra-terminal flows of the containers in order to increasing the performance of the hub under economic and environmental perspective. Originality The adoption of a dry port can effectively solve the congestion problem of a terminal only if an integrated sustainable solution (dry port location and container strategy storage) is identified. Research method The model is based on a heuristic computational algorithm for non-linear programming. Main findings The application of DSS to a full-scale numerical case show the model capabilities in identifying the optimal logistic strategies ensuring a low CF and in optimizing the cost due to transport activities. Implications for theory and practice It is possible to identify different strategies allowing to obtain an eco-friendly solution reducing, at same time, the costs for a given number of containers to be handled.
DOI: 10.6084/m9.figshare.6992057
title: Sub-Minute Method for Determination of Naphazoline in the Presence of Diphenhydramine, Pheniramine or Chlorpheniramine by Capillary Electrophoresis

description: This paper presents a simple and low-cost capillary electrophoresis method for ultra-fast simultaneous determination of naphazoline (NPZ) and one of the following active ingredients: diphenhydramine (DIP), pheniramine (PHEN) or chlorpheniramine (CPHEN). The proposed method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) equipped with a short length capillary column (10 cm). One sample can be analyzed every 35 s (ca. 100 injections per hour) with resolutions between peaks greater than 1.4. The optimized background electrolyte (BGE) was composed by 20 mmol L-1 histidine and 10 mmol L-1 3,4-dimethoxycinnamic acid (pH = 9.5, adjusted with NaOH). Limits of detection were 25 µmol L-1 for NPZ, DIP, and PHEN and 13 µmol L-1 for CPHEN. The results obtained with the developed procedure were compared to those obtained by high-performance liquid chromatography (HPLC) and no statistically significant differences were observed (95% confidence level).
DOI: 10.6084/m9.figshare.6992057.v1
title: Sub-Minute Method for Determination of Naphazoline in the Presence of Diphenhydramine, Pheniramine or Chlorpheniramine by Capillary Electrophoresis

description: This paper presents a simple and low-cost capillary electrophoresis method for ultra-fast simultaneous determination of naphazoline (NPZ) and one of the following active ingredients: diphenhydramine (DIP), pheniramine (PHEN) or chlorpheniramine (CPHEN). The proposed method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) equipped with a short length capillary column (10 cm). One sample can be analyzed every 35 s (ca. 100 injections per hour) with resolutions between peaks greater than 1.4. The optimized background electrolyte (BGE) was composed by 20 mmol L-1 histidine and 10 mmol L-1 3,4-dimethoxycinnamic acid (pH = 9.5, adjusted with NaOH). Limits of detection were 25 µmol L-1 for NPZ, DIP, and PHEN and 13 µmol L-1 for CPHEN. The results obtained with the developed procedure were compared to those obtained by high-performance liquid chromatography (HPLC) and no statistically significant differences were observed (95% confidence level).
DOI: 10.6084/m9.figshare.6992051
title: Nitrogen management in wheat based on the normalized difference vegetation index (NDVI)

description: ABSTRACT: Biomass production and nitrogen (N) accumulated in wheat shoots may be used for quantifying optimal topdressing nitrogen doses. The objective of this study was to develop and validate models for estimating the amount of biomass and nitrogen accumulated in shoots and the N topdressing dose of maximum technical efficiency in wheat using the normalized difference vegetation index (NDVI) measured by an active optical canopy sensor. Field experiments were carried out in two years and treatments consisted of N doses applied at plant emergence and as topdressing. NDVI, shoot biomass and N accumulated in shoots at the growth stage of six fully expanded leaves and grain yield were evaluated, being determined the topdressing N dose of maximum technical efficiency (DMTE). The NDVI was positively correlated to shoot biomass and N content in shoots and models for the relationship between these variables were developed and validated. The DMTE was negatively correlated with the NDVI value evaluated at the moment of N topdressing application. Thus, NDVI evaluation by an active optical canopy sensor can be used for nitrogen fertilization in variable rate, allowing the adjustment of applied N doses in different areas within a field.
DOI: 10.6084/m9.figshare.6992048
title: Inflammation and kidney injury attenuated by prior intake of Brazil nuts in the process of ischemia and reperfusion

description: ABSTRACT Introduction: Ischemia and reperfusion (IR) is a process inherent to the procedures involved in the transplantation of organs that causes inflammation, cell death and cell injury, and may lead to rejection of the graft. It is possible that the anti-inflammatory properties of the Brazil nuts (BN) can mitigate the renal injury caused by IR. Objective: To investigate whether the previous intake of BN reduces the expression of markers of inflammation, injury, and cell death after renal IR. Methods: Male Wistar rats were distributed into six groups (N = 6/group): SHAM (control), SHAM treated with 75 or 150 mg of BN, IR, and IR treated with 75 or 150 mg of BN. The IR procedure consisted of right nephrectomy and occlusion of the left renal artery with a non-traumatic vascular clamp for 30 min. BN was given daily from day 1 to 7 before surgery (SHAM or IR), and maintained until sacrifice (48 h after surgery). Inflammation was evaluated by renal expression of COX-2 and TGF-β, injury by the expression of vimentin, and cell death by apoptosis through caspase-3 expression (immunohistochemistry). Results: Pretreatment with 75 mg of BN reduced renal expression of the COX-2, TGF-β, vimentin, and caspase-3. The dose of 150 mg caused increased expression of COX-2. Conclusion: In experimental IR, the damage can be minimized with a prior low-dose intake of BN, improving inflammation, injury, and cell death.
DOI: 10.6084/m9.figshare.6992051.v1
title: Nitrogen management in wheat based on the normalized difference vegetation index (NDVI)

description: ABSTRACT: Biomass production and nitrogen (N) accumulated in wheat shoots may be used for quantifying optimal topdressing nitrogen doses. The objective of this study was to develop and validate models for estimating the amount of biomass and nitrogen accumulated in shoots and the N topdressing dose of maximum technical efficiency in wheat using the normalized difference vegetation index (NDVI) measured by an active optical canopy sensor. Field experiments were carried out in two years and treatments consisted of N doses applied at plant emergence and as topdressing. NDVI, shoot biomass and N accumulated in shoots at the growth stage of six fully expanded leaves and grain yield were evaluated, being determined the topdressing N dose of maximum technical efficiency (DMTE). The NDVI was positively correlated to shoot biomass and N content in shoots and models for the relationship between these variables were developed and validated. The DMTE was negatively correlated with the NDVI value evaluated at the moment of N topdressing application. Thus, NDVI evaluation by an active optical canopy sensor can be used for nitrogen fertilization in variable rate, allowing the adjustment of applied N doses in different areas within a field.