cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20038243
title: Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

description: Abstract Peanut (Arachis hypogaea) is an important legume and oilseed crop, native to South America and grown in all tropical and temperate regions of the world. A simplified and rapid direct gene delivery system in peanut was developed by vortexing silicon carbide whiskers with callus and with plasmid harboring chitinase and hygromcin genes. The effects of callus age and whisker quantity on transformation efficiency were evaluated. Transformation efficiency (6.88%) was highest when 200 mg of whiskers were used with 5 µg plasmid for 2 g of 20-day-old callus. Hygromcin-resistant calli were regenerated to complete plants which produced seeds normally. Transgene insertion and number of transgene copieswere confirmed by PCR and southern blot analyses, respectively. Transgene expression was evaluated by a pathogenecity test and RT-PCR analysis. In transgenic events, the resistance level to leaf spot disease was far higher than in control plants.
DOI: 10.6084/m9.figshare.20038246
title: Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

description: Abstract The known sources of resistance to witches' broom (WB), a severe disease of cacao, are limited. Aiming to identify families and parents resistant to Moniliophthora perniciosa, a population of 22 families was evaluated by assessing the number of brooms formed per tree during 10 years under field conditions. The population was established in randomized blocks with three replications of 12 plants each. Significant differences were observed among families. The most outstanding families were NA33 x RB39 and RB39 x P4B, which presented the lowest WB incidence during 10 years. The increase in natural field infection of Scavina clones families and their descendants were clearly demonstrated after 2006. The existence of additive effects for resistance appears clearly for families, which had other source of resistance associated with Scavina. Clones RB39, RB36, P4B, NA33 and CSUL3 are promising parents for pyramiding resistance genes and increasing the stability and durability of resistance to WB.
DOI: 10.6084/m9.figshare.20038246.v1
title: Cacao families and parents selected as resistant to natural infection of Moniliophthora perniciosa

description: Abstract The known sources of resistance to witches' broom (WB), a severe disease of cacao, are limited. Aiming to identify families and parents resistant to Moniliophthora perniciosa, a population of 22 families was evaluated by assessing the number of brooms formed per tree during 10 years under field conditions. The population was established in randomized blocks with three replications of 12 plants each. Significant differences were observed among families. The most outstanding families were NA33 x RB39 and RB39 x P4B, which presented the lowest WB incidence during 10 years. The increase in natural field infection of Scavina clones families and their descendants were clearly demonstrated after 2006. The existence of additive effects for resistance appears clearly for families, which had other source of resistance associated with Scavina. Clones RB39, RB36, P4B, NA33 and CSUL3 are promising parents for pyramiding resistance genes and increasing the stability and durability of resistance to WB.
DOI: 10.6084/m9.figshare.20038247
title: Characterization and selection of interspecific hybrids of Brachiaria decumbens for seed production in Campo Grande - MS

description: Abstract The breeding program Brachiaria developed by Embrapa Beef Cattle provides studies to obtain forage with agronomic characters desired by farmers. In this regard, in 2013 and 2014, a study was carried out in order to select the best Brachiaria decumbens intraspecific hybrids, which are superior in relation to production, forage nutritional value, and resistance to spittlebugs. Estimates of genetic parameters and gains with selection were carried out. It was found that there was significant variability between genotypes for all characters. Gain with selection (GS%) ranged from 12 to 324%, and the highest percentage was found for weight of pure seeds (PS) of seed collectors of the second harvest. For reproductive tillers (RT), C001 and R091 hybrids had better performance than the control, and this character may be considered as a parameter to estimate production of pure seeds before flowering starts.
DOI: 10.6084/m9.figshare.20038247.v1
title: Characterization and selection of interspecific hybrids of Brachiaria decumbens for seed production in Campo Grande - MS

description: Abstract The breeding program Brachiaria developed by Embrapa Beef Cattle provides studies to obtain forage with agronomic characters desired by farmers. In this regard, in 2013 and 2014, a study was carried out in order to select the best Brachiaria decumbens intraspecific hybrids, which are superior in relation to production, forage nutritional value, and resistance to spittlebugs. Estimates of genetic parameters and gains with selection were carried out. It was found that there was significant variability between genotypes for all characters. Gain with selection (GS%) ranged from 12 to 324%, and the highest percentage was found for weight of pure seeds (PS) of seed collectors of the second harvest. For reproductive tillers (RT), C001 and R091 hybrids had better performance than the control, and this character may be considered as a parameter to estimate production of pure seeds before flowering starts.
DOI: 10.6084/m9.figshare.20038252
title: Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera

description: Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of these plants revealed the presence of the cry2AX1 gene in 40 plants. Southern blot hybridisation analysis of selected transgenic plants confirmed stable T-DNA integration in the genome of transformed plants. The level of Cry2AX1 protein expression in PCR positive plants ranged from 4.9 to 187.5 ng g-1 of fresh tissue. A transgenic cotton event, TP31, expressing the cry2AX1 gene showed insecticidal activity of 56.66 per cent mortality against Helicoverpa armigera in detached leaf disc bioassay. These results indicate that the chimeric cry2AX1 gene expressed in transgenic cotton has insecticidal activity against H. armigera.
DOI: 10.6084/m9.figshare.20038252.v1
title: Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera

description: Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of these plants revealed the presence of the cry2AX1 gene in 40 plants. Southern blot hybridisation analysis of selected transgenic plants confirmed stable T-DNA integration in the genome of transformed plants. The level of Cry2AX1 protein expression in PCR positive plants ranged from 4.9 to 187.5 ng g-1 of fresh tissue. A transgenic cotton event, TP31, expressing the cry2AX1 gene showed insecticidal activity of 56.66 per cent mortality against Helicoverpa armigera in detached leaf disc bioassay. These results indicate that the chimeric cry2AX1 gene expressed in transgenic cotton has insecticidal activity against H. armigera.
DOI: 10.6084/m9.figshare.20038253
title: RNAi-mediated resistance to SMV and BYMV in transgenic tobacco

description: Abstract Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the control plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam.
DOI: 10.6084/m9.figshare.20038253.v1
title: RNAi-mediated resistance to SMV and BYMV in transgenic tobacco

description: Abstract Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the control plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam.
DOI: 10.6084/m9.figshare.20038255
title: Genetic and cytological diversity in cherry tree accessions ( Eugenia involucrata DC) in Rio Grande do Sul

description: Abstract This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diversity allowed the separation of accessions into four groups, resulting in an average of 8.93 bands per primer amplified, 7.89 polymorphic bands, 88.08% of polymorphism and 86% of genetic similarity. Cytological analyses of gametic cells allowed for the characterization of accessions as diploids with n=11. In these, the average of meiotic cells considered normal was 82.12%; average pollen viability was 92.44% and in vitro germination was 40.26%; the average number of anthers was 161.85 anthers/flowers. Therefore, the accessions evaluated showed high genetic similarity and cytological stability and can be used in commercial plantations or hybridizations.
DOI: 10.6084/m9.figshare.20038255.v1
title: Genetic and cytological diversity in cherry tree accessions ( Eugenia involucrata DC) in Rio Grande do Sul

description: Abstract This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diversity allowed the separation of accessions into four groups, resulting in an average of 8.93 bands per primer amplified, 7.89 polymorphic bands, 88.08% of polymorphism and 86% of genetic similarity. Cytological analyses of gametic cells allowed for the characterization of accessions as diploids with n=11. In these, the average of meiotic cells considered normal was 82.12%; average pollen viability was 92.44% and in vitro germination was 40.26%; the average number of anthers was 161.85 anthers/flowers. Therefore, the accessions evaluated showed high genetic similarity and cytological stability and can be used in commercial plantations or hybridizations.
DOI: 10.6084/m9.figshare.20038256.v1
title: Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

description: Abstract This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.
DOI: 10.6084/m9.figshare.20038256
title: Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

description: Abstract This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.
DOI: 10.6084/m9.figshare.20038258
title: UENF Rio Dourado: a new passion fruit cultivar with high yield potential

description: Abstract This work aimed at introducing the characteristics of the passion fruit cultivar UENF Rio Dourado, developed from three cycles of recurrent selection, to the scientific community. The cultivar presents yield of 25 tons ha-1, mean values of 250 for number of fruits and 175g for fruit weight, providing a percentage increase in yield, number of fruits and fruit weight of 36%, 25% and 3%, respectively, when compared to the standard cultivar tested.
DOI: 10.6084/m9.figshare.20038258.v1
title: UENF Rio Dourado: a new passion fruit cultivar with high yield potential

description: Abstract This work aimed at introducing the characteristics of the passion fruit cultivar UENF Rio Dourado, developed from three cycles of recurrent selection, to the scientific community. The cultivar presents yield of 25 tons ha-1, mean values of 250 for number of fruits and 175g for fruit weight, providing a percentage increase in yield, number of fruits and fruit weight of 36%, 25% and 3%, respectively, when compared to the standard cultivar tested.
DOI: 10.6084/m9.figshare.20038261.v1
title: QTL mapping for yield components and agronomic traits in a Brazilian soybean population

description: Abstract The objective of this work was to map QTL for agronomic traits in a Brazilian soybean population. For this, 207 F2:3 progenies from the cross CS3035PTA276-1-5-2 x UFVS2012 were genotyped and cultivated in Viçosa-MG, using randomized block design with three replications. QTL detection was carried out by linear regression and composite interval mapping. Thirty molecular markers linked to QTL were detected by linear regression for the total of nine agronomic traits. QTL for SWP (seed weight per plant), W100S (weight of 100 seeds), NPP (number of pods per plant), and NSP (number of seeds per plant) were detected by composite interval mapping. Four QTL with additive effect are promising for marker-assisted selection (MAS). Particularly, the markers Satt155 and Satt300 could be useful in simultaneous selection for greater SWP, NPP, and NSP.
DOI: 10.6084/m9.figshare.20038261
title: QTL mapping for yield components and agronomic traits in a Brazilian soybean population

description: Abstract The objective of this work was to map QTL for agronomic traits in a Brazilian soybean population. For this, 207 F2:3 progenies from the cross CS3035PTA276-1-5-2 x UFVS2012 were genotyped and cultivated in Viçosa-MG, using randomized block design with three replications. QTL detection was carried out by linear regression and composite interval mapping. Thirty molecular markers linked to QTL were detected by linear regression for the total of nine agronomic traits. QTL for SWP (seed weight per plant), W100S (weight of 100 seeds), NPP (number of pods per plant), and NSP (number of seeds per plant) were detected by composite interval mapping. Four QTL with additive effect are promising for marker-assisted selection (MAS). Particularly, the markers Satt155 and Satt300 could be useful in simultaneous selection for greater SWP, NPP, and NSP.
DOI: 10.6084/m9.figshare.20038265.v1
title: Selection in energy cane families

description: Abstract The objective of this study was to identify superior parents for crosses and the best families to breed new clones of energy cane. The best-performing parents were RB867515, RB93509, KRAKATAU, IM76-228, IM76-229, and US85-1008. The heritability and accuracy values for the traits tons of cane per hectare, fiber, and sucrose content indicate high correlation between the predicted genotypic means and observed values, enabling efficient selection of the best cane energy families. The extensive genetic variation detected and the presence of promising seedlings in the selected segregating population indicate the possibility of using new crosses in some clones and even in future commercial plantings. It was possible to identify the best parents involved in the evaluated crosses, and to select, in the best families, seedlings with a high fiber and good sucrose content, seedlings with high fiber and low sugar content, as well as seedlings with the same fiber and sucrose contents as the current cultivars.
DOI: 10.6084/m9.figshare.20038265
title: Selection in energy cane families

description: Abstract The objective of this study was to identify superior parents for crosses and the best families to breed new clones of energy cane. The best-performing parents were RB867515, RB93509, KRAKATAU, IM76-228, IM76-229, and US85-1008. The heritability and accuracy values for the traits tons of cane per hectare, fiber, and sucrose content indicate high correlation between the predicted genotypic means and observed values, enabling efficient selection of the best cane energy families. The extensive genetic variation detected and the presence of promising seedlings in the selected segregating population indicate the possibility of using new crosses in some clones and even in future commercial plantings. It was possible to identify the best parents involved in the evaluated crosses, and to select, in the best families, seedlings with a high fiber and good sucrose content, seedlings with high fiber and low sugar content, as well as seedlings with the same fiber and sucrose contents as the current cultivars.
DOI: 10.6084/m9.figshare.20038267
title: Cytoembryological evaluation, meiotic behavior and pollen viability of Paspalum notatum tetraploidized plants

description: Abstract This study evaluated the mode of reproduction, the meiotic behavior and the pollen viability of three tetraploid plants (2n=4x=40) originated from somatic chromosome duplication of Paspalum notatum plants. The plant WKS 3 changed the mode of reproduction after duplication and became apomictic. The plants WKS 63 and WKS 92 confirmed sexual mode of reproduction identical to that of the original genotype. The analyzed plants presented meiotic abnormalities related to tetraploidy, and the chromosome pairing were variable, but it did not hinder the meiotic products, which were characterized by regular tetrads and satisfactory pollen fertility, ranging from 88.7 to 95.7%. Results show that all plants are meiotically stable and that they can be used in intraspecific crosses in the breeding program of Paspalum notatum.
DOI: 10.6084/m9.figshare.20038267.v1
title: Cytoembryological evaluation, meiotic behavior and pollen viability of Paspalum notatum tetraploidized plants

description: Abstract This study evaluated the mode of reproduction, the meiotic behavior and the pollen viability of three tetraploid plants (2n=4x=40) originated from somatic chromosome duplication of Paspalum notatum plants. The plant WKS 3 changed the mode of reproduction after duplication and became apomictic. The plants WKS 63 and WKS 92 confirmed sexual mode of reproduction identical to that of the original genotype. The analyzed plants presented meiotic abnormalities related to tetraploidy, and the chromosome pairing were variable, but it did not hinder the meiotic products, which were characterized by regular tetrads and satisfactory pollen fertility, ranging from 88.7 to 95.7%. Results show that all plants are meiotically stable and that they can be used in intraspecific crosses in the breeding program of Paspalum notatum.
DOI: 10.6084/m9.figshare.20038271
title: Tissue culture efficiency of wheat species with different genomic formulas

description: Abstract Ancient wheats are increasingly considered as valuable resources for genes of interest which could be analyzed and introduced into cultivated varieties by genetic engineering technologies. The first stage of biotechnological crop improvement consists of successful in vitro plant regeneration. Twelve wheat germplasms with different genomic formulas (AA, AABB, AAGG, AABBDD, AADDGG genomes) were examined with the use of two explant types (immature vs. mature embryos). All of the tested germplasms were able to regenerate plants, although the morphogenic ability of immature embryos was higher. The highest rate of embryogenic/regenerable structure formation was found in immature embryo cultures of tetraploid species (T. polonicum, T. turgidum, T. carthlicum, and T. dicoccum) as well as of hexaploid T. spelta. At the same time, diploid einkorn wheat (T. monococcum) and polyploid species with G chromosomes (T. timopheevii and T. kiharae) were characterized by low embryogenesis and by the presence of albino plantlets among shoots.
DOI: 10.6084/m9.figshare.20038271.v1
title: Tissue culture efficiency of wheat species with different genomic formulas

description: Abstract Ancient wheats are increasingly considered as valuable resources for genes of interest which could be analyzed and introduced into cultivated varieties by genetic engineering technologies. The first stage of biotechnological crop improvement consists of successful in vitro plant regeneration. Twelve wheat germplasms with different genomic formulas (AA, AABB, AAGG, AABBDD, AADDGG genomes) were examined with the use of two explant types (immature vs. mature embryos). All of the tested germplasms were able to regenerate plants, although the morphogenic ability of immature embryos was higher. The highest rate of embryogenic/regenerable structure formation was found in immature embryo cultures of tetraploid species (T. polonicum, T. turgidum, T. carthlicum, and T. dicoccum) as well as of hexaploid T. spelta. At the same time, diploid einkorn wheat (T. monococcum) and polyploid species with G chromosomes (T. timopheevii and T. kiharae) were characterized by low embryogenesis and by the presence of albino plantlets among shoots.