cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1571350
title: SHCBP1 is required for midbody organization and cytokinesis completion

description: The centralspindlin complex, which is composed of MKLP1 and MgcRacGAP, is one of the crucial factors involved in cytokinesis initiation. Centralspindlin is localized at the middle of the central spindle during anaphase and then concentrates at the midbody to control abscission. A number of proteins that associate with centralspindlin have been identified. These associating factors regulate furrowing and abscission in coordination with centralspindlin. A recent study identified a novel centralspindlin partner, called Nessun Dorma, which is essential for germ cell cytokinesis in <i>Drosophila melanogaster</i>. SHCBP1 is a human ortholog of Nessun Dorma that associates with human centralspindlin. In this report, we analyzed the interaction of SHCBP1 with centralspindlin in detail and determined the regions that are required for the interaction. In addition, we demonstrate that the central region is necessary for the SHCBP1 dimerization. Both MgcRacGAP and MKLP1 are degraded once cells exit mitosis. Similarly, endogenous and exogenous SHCBP1 were degraded with mitosis progression. Interestingly, SHCBP1 expression was significantly reduced in the absence of centralspindlin, whereas centralspindlin expression was not affected by SHCBP1 knockdown. Finally, we demonstrate that SHCBP1 depletion promotes midbody structure disruption and inhibits abscission, a final stage of cytokinesis. Our study gives novel insight into the role of SHCBP in cytokinesis completion.
DOI: 10.6084/m9.figshare.1571350.v1
title: SHCBP1 is required for midbody organization and cytokinesis completion

description: The centralspindlin complex, which is composed of MKLP1 and MgcRacGAP, is one of the crucial factors involved in cytokinesis initiation. Centralspindlin is localized at the middle of the central spindle during anaphase and then concentrates at the midbody to control abscission. A number of proteins that associate with centralspindlin have been identified. These associating factors regulate furrowing and abscission in coordination with centralspindlin. A recent study identified a novel centralspindlin partner, called Nessun Dorma, which is essential for germ cell cytokinesis in <i>Drosophila melanogaster</i>. SHCBP1 is a human ortholog of Nessun Dorma that associates with human centralspindlin. In this report, we analyzed the interaction of SHCBP1 with centralspindlin in detail and determined the regions that are required for the interaction. In addition, we demonstrate that the central region is necessary for the SHCBP1 dimerization. Both MgcRacGAP and MKLP1 are degraded once cells exit mitosis. Similarly, endogenous and exogenous SHCBP1 were degraded with mitosis progression. Interestingly, SHCBP1 expression was significantly reduced in the absence of centralspindlin, whereas centralspindlin expression was not affected by SHCBP1 knockdown. Finally, we demonstrate that SHCBP1 depletion promotes midbody structure disruption and inhibits abscission, a final stage of cytokinesis. Our study gives novel insight into the role of SHCBP in cytokinesis completion.
DOI: 10.6084/m9.figshare.1571357
title: Centralspindlin assembly and 2 phosphorylations on MgcRacGAP by Polo-like kinase 1 initiate Ect2 binding in early cytokinesis

description: Cytokinesis is the final step of cell division which partitions genetic and cytosolic content into daughter cells. Failed cytokinesis causes polyploidy, genetic instability, and cancer. Kinases use phosphorylation to regulate the timing and location of the cytokinetic furrow. Polo-like kinase 1 (Plk1) is an essential mitotic kinase that triggers cytokinesis by phosphorylating MgcRacGAP to create a docking site for Ect2 at the central spindle. Ect2 binds to MgcRacGAP via its N-terminal BRCT domain (BRCA1 C-terminal), which docks at specific phosphorylated residues. Here we investigate the minimal Plk1-dependent phosphorylation sites required for cytokinesis onset. We demonstrate that phosphorylation of the major MgcRacGAP site, S157, is necessary but not sufficient to bind the Ect2 BRCT domain. Phosphorylation of an additional residue on MgcRacGAP at S164 is also required to elicit efficient binding. Surprisingly, BRCT binding additionally requires MKLP1 and its cognate interacting N-terminal domain of MgcRacGAP. Our findings indicate that central spindle assembly and 2 Plk1-dependent phosphorylations are required to establish efficient binding of the Ect2 BRCT in early cytokinesis. We propose that these requirements establish a high threshold to restrain premature or ectopic cytokinesis.
DOI: 10.6084/m9.figshare.1571357.v1
title: Centralspindlin assembly and 2 phosphorylations on MgcRacGAP by Polo-like kinase 1 initiate Ect2 binding in early cytokinesis

description: Cytokinesis is the final step of cell division which partitions genetic and cytosolic content into daughter cells. Failed cytokinesis causes polyploidy, genetic instability, and cancer. Kinases use phosphorylation to regulate the timing and location of the cytokinetic furrow. Polo-like kinase 1 (Plk1) is an essential mitotic kinase that triggers cytokinesis by phosphorylating MgcRacGAP to create a docking site for Ect2 at the central spindle. Ect2 binds to MgcRacGAP via its N-terminal BRCT domain (BRCA1 C-terminal), which docks at specific phosphorylated residues. Here we investigate the minimal Plk1-dependent phosphorylation sites required for cytokinesis onset. We demonstrate that phosphorylation of the major MgcRacGAP site, S157, is necessary but not sufficient to bind the Ect2 BRCT domain. Phosphorylation of an additional residue on MgcRacGAP at S164 is also required to elicit efficient binding. Surprisingly, BRCT binding additionally requires MKLP1 and its cognate interacting N-terminal domain of MgcRacGAP. Our findings indicate that central spindle assembly and 2 Plk1-dependent phosphorylations are required to establish efficient binding of the Ect2 BRCT in early cytokinesis. We propose that these requirements establish a high threshold to restrain premature or ectopic cytokinesis.
DOI: 10.6084/m9.figshare.1572119
title: Study on the formation process for Co<sup>2+</sup>-Ni<sup>2+</sup>-Fe<sup>3+</sup>-CO<sub>3</sub><sup>2-</sup> LDHs prepared by trisodium citrate complexing agent-assisted homogeneous precipitation method

description: A new complexing agent-assisted homogeneous precipitation technology was successfully developed to synthesize Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials with high crystallinity and well-defined hexagonal shapes. An experiment to trace changes of phase composition, morphological features of crystallinity, molar ratio of Co<sup>2+</sup>/Ni<sup>2+</sup>/Fe<sup>3+</sup>, and the pH changes in the reaction system was designed to investigate the formation process of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials. The formation process was proposed on the basis of the experimental results. In the case of no TSC added, Fe<sup>3+</sup> ions in the reaction system precipitated to Fe(OH)<sub>3</sub> at low pH value, and  the Fe(OH)<sub>3</sub> is hard to convert to Co<sup>2+</sup>-Ni<sup>2+</sup>-Fe<sup>3+</sup>-- LDHs materials of highly crystalline and regular shapes with free Co<sup>2+</sup> and Ni<sup>2+</sup>. In addition, <i>β</i>-Ni(OH)<sub>2</sub> as an impurity phase is easily formed in company with the formation of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs. When TSC is added and optimized amount was 0.6–1.0 mmol L<sup>−1</sup>, a parts of Fe<sup>3+</sup> ions complex with TSC form the [Fe(C<sub>6</sub>H<sub>4</sub>O<sub>7</sub>)<sub>2</sub>]<sup>5−</sup> ligand ions and reduce the amount production of Fe(OH)<sub>3</sub>, causing pH deposition range of Fe<sup>3+</sup> rise. Moreover, the formed [Fe(C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>)<sub>2</sub>]<sup>5−</sup> ligand ions changed the direct conversion process of Fe(OH)<sub>3</sub> to Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs, which reacted with free Co<sup>2+</sup>, Ni<sup>2+</sup> ions and formed Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials with high crystallinity and well-defined hexagonal shapes in the higher pH value of the reaction system. While TSC optimized amount has more than 1.5 mmol L<sup>−1</sup>, the excess TSC also complex some of Ni<sup>2+</sup> ions except for Fe<sup>3+</sup> in the solution and also induce pH deposition range of Ni<sup>2+</sup> raise, which is not favorable to formation of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs material because Ni<sup>2+</sup> still existed in the complex form even in the higher precipitation pH value of the reaction system.
DOI: 10.6084/m9.figshare.1572119.v1
title: Study on the formation process for Co<sup>2+</sup>-Ni<sup>2+</sup>-Fe<sup>3+</sup>-CO<sub>3</sub><sup>2-</sup> LDHs prepared by trisodium citrate complexing agent-assisted homogeneous precipitation method

description: A new complexing agent-assisted homogeneous precipitation technology was successfully developed to synthesize Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials with high crystallinity and well-defined hexagonal shapes. An experiment to trace changes of phase composition, morphological features of crystallinity, molar ratio of Co<sup>2+</sup>/Ni<sup>2+</sup>/Fe<sup>3+</sup>, and the pH changes in the reaction system was designed to investigate the formation process of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials. The formation process was proposed on the basis of the experimental results. In the case of no TSC added, Fe<sup>3+</sup> ions in the reaction system precipitated to Fe(OH)<sub>3</sub> at low pH value, and  the Fe(OH)<sub>3</sub> is hard to convert to Co<sup>2+</sup>-Ni<sup>2+</sup>-Fe<sup>3+</sup>-- LDHs materials of highly crystalline and regular shapes with free Co<sup>2+</sup> and Ni<sup>2+</sup>. In addition, <i>β</i>-Ni(OH)<sub>2</sub> as an impurity phase is easily formed in company with the formation of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs. When TSC is added and optimized amount was 0.6–1.0 mmol L<sup>−1</sup>, a parts of Fe<sup>3+</sup> ions complex with TSC form the [Fe(C<sub>6</sub>H<sub>4</sub>O<sub>7</sub>)<sub>2</sub>]<sup>5−</sup> ligand ions and reduce the amount production of Fe(OH)<sub>3</sub>, causing pH deposition range of Fe<sup>3+</sup> rise. Moreover, the formed [Fe(C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>)<sub>2</sub>]<sup>5−</sup> ligand ions changed the direct conversion process of Fe(OH)<sub>3</sub> to Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs, which reacted with free Co<sup>2+</sup>, Ni<sup>2+</sup> ions and formed Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials with high crystallinity and well-defined hexagonal shapes in the higher pH value of the reaction system. While TSC optimized amount has more than 1.5 mmol L<sup>−1</sup>, the excess TSC also complex some of Ni<sup>2+</sup> ions except for Fe<sup>3+</sup> in the solution and also induce pH deposition range of Ni<sup>2+</sup> raise, which is not favorable to formation of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs material because Ni<sup>2+</sup> still existed in the complex form even in the higher precipitation pH value of the reaction system.
DOI: 10.6084/m9.figshare.1572119.v2
title: Study on the formation process for Co<sup>2+</sup>-Ni<sup>2+</sup>-Fe<sup>3+</sup>-CO<sub>3</sub><sup>2-</sup> LDHs prepared by trisodium citrate complexing agent-assisted homogeneous precipitation method

description: A new complexing agent-assisted homogeneous precipitation technology was successfully developed to synthesize Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials with high crystallinity and well-defined hexagonal shapes. An experiment to trace changes of phase composition, morphological features of crystallinity, molar ratio of Co<sup>2+</sup>/Ni<sup>2+</sup>/Fe<sup>3+</sup>, and the pH changes in the reaction system was designed to investigate the formation process of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials. The formation process was proposed on the basis of the experimental results. In the case of no TSC added, Fe<sup>3+</sup> ions in the reaction system precipitated to Fe(OH)<sub>3</sub> at low pH value, and  the Fe(OH)<sub>3</sub> is hard to convert to Co<sup>2+</sup>-Ni<sup>2+</sup>-Fe<sup>3+</sup>-- LDHs materials of highly crystalline and regular shapes with free Co<sup>2+</sup> and Ni<sup>2+</sup>. In addition, <i>β</i>-Ni(OH)<sub>2</sub> as an impurity phase is easily formed in company with the formation of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs. When TSC is added and optimized amount was 0.6–1.0 mmol L<sup>−1</sup>, a parts of Fe<sup>3+</sup> ions complex with TSC form the [Fe(C<sub>6</sub>H<sub>4</sub>O<sub>7</sub>)<sub>2</sub>]<sup>5−</sup> ligand ions and reduce the amount production of Fe(OH)<sub>3</sub>, causing pH deposition range of Fe<sup>3+</sup> rise. Moreover, the formed [Fe(C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>)<sub>2</sub>]<sup>5−</sup> ligand ions changed the direct conversion process of Fe(OH)<sub>3</sub> to Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs, which reacted with free Co<sup>2+</sup>, Ni<sup>2+</sup> ions and formed Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs materials with high crystallinity and well-defined hexagonal shapes in the higher pH value of the reaction system. While TSC optimized amount has more than 1.5 mmol L<sup>−1</sup>, the excess TSC also complex some of Ni<sup>2+</sup> ions except for Fe<sup>3+</sup> in the solution and also induce pH deposition range of Ni<sup>2+</sup> raise, which is not favorable to formation of Co<sup>2+</sup>–Ni<sup>2+</sup>–Fe<sup>3+</sup>– LDHs material because Ni<sup>2+</sup> still existed in the complex form even in the higher precipitation pH value of the reaction system.
DOI: 10.6084/m9.figshare.1572130
title: Characterization of PM<sub>2.5</sub> and PM<sub>10</sub> fugitive dust source profiles in the Athabasca Oil Sands Region

description: Geological samples were collected from 27 representative locations in the Athabasca Oil Sands Region (AOSR) in Alberta, Canada. These samples were resuspended onto filter substrates for PM<sub>2.5</sub> and PM<sub>10</sub> size fractions. Samples were analyzed for 229 chemical species, consisting of elements, ions, carbon, and organic compounds. These chemical species are normalized to gravimetric mass to derive individual source profiles. Individual profiles were grouped into six categories typical of those used in emission inventories: paved road dust, unpaved road dust close to and distant from oil sand operations, overburden soil, tailings sands, and forest soils. Consistent with their geological origin, the major components are minerals, organic and elemental carbon, and ions. The sum of five major elements (i.e., Al, Si, K, Ca, and Fe) and their oxidized forms account for 25–40% and 45–82% of particulate matter (PM) mass, respectively. Si is the most abundant element, averaging 17–18% in the Facility (oil sand operations) and 23–27% in the Forest profiles. Organic carbon is the second most abundant species, averaging 9–11% in the Facility and 5–6% in the Forest profiles. Elemental carbon abundance is 2–3 times higher in Facility than Forest profiles. Sulfate abundance is ~7 times higher in the Facility than in the Forest profiles. The ratios of cation/anion and base cation (sum of Na<sup>+</sup>, Mg<sup>2+</sup>, K<sup>+</sup>, and Ca<sup>2+</sup>)/nitrogen- and sulfur-containing ions (sum of NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub><sup>-</sup>, NO<sub>3</sub><sup>-</sup>, and SO<sub>4</sub><sup>2-</sup>) exceed unity, indicating that the soils are basic. Lead (Pb) isotope ratios of facility soils are similar to the AOSR stack and diesel emissions, while those of forest soils have much lower <sup>206</sup>Pb/<sup>207</sup>Pb and <sup>208</sup>Pb/<sup>207</sup>Pb ratios. High-molecular-weight <i>n</i>-alkanes (C<sub>25</sub>-C<sub>40</sub>), hopanes, and steranes are more than an order of magnitude more abundant in Facility than Forest profiles. These differences may be useful for separating anthropogenic from natural sources of fugitive dust at receptors.Implications: <i>Several organic compounds typical of combustion emissions and bitumen are enriched relative to forest soils for fugitive dust sources near oil sands operations, consistent with deposition uptake by biomonitors. AOSR dust samples are alkaline, not acidic, indicating that potential acid deposition is neutralized. Chemical abundances are highly variable within emission inventory categories, implying that more specific subcategories can be defined for inventory speciation.</i>
DOI: 10.6084/m9.figshare.1572130.v2
title: Characterization of PM<sub>2.5</sub> and PM<sub>10</sub> fugitive dust source profiles in the Athabasca Oil Sands Region

description: Geological samples were collected from 27 representative locations in the Athabasca Oil Sands Region (AOSR) in Alberta, Canada. These samples were resuspended onto filter substrates for PM<sub>2.5</sub> and PM<sub>10</sub> size fractions. Samples were analyzed for 229 chemical species, consisting of elements, ions, carbon, and organic compounds. These chemical species are normalized to gravimetric mass to derive individual source profiles. Individual profiles were grouped into six categories typical of those used in emission inventories: paved road dust, unpaved road dust close to and distant from oil sand operations, overburden soil, tailings sands, and forest soils. Consistent with their geological origin, the major components are minerals, organic and elemental carbon, and ions. The sum of five major elements (i.e., Al, Si, K, Ca, and Fe) and their oxidized forms account for 25–40% and 45–82% of particulate matter (PM) mass, respectively. Si is the most abundant element, averaging 17–18% in the Facility (oil sand operations) and 23–27% in the Forest profiles. Organic carbon is the second most abundant species, averaging 9–11% in the Facility and 5–6% in the Forest profiles. Elemental carbon abundance is 2–3 times higher in Facility than Forest profiles. Sulfate abundance is ~7 times higher in the Facility than in the Forest profiles. The ratios of cation/anion and base cation (sum of Na<sup>+</sup>, Mg<sup>2+</sup>, K<sup>+</sup>, and Ca<sup>2+</sup>)/nitrogen- and sulfur-containing ions (sum of NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub><sup>-</sup>, NO<sub>3</sub><sup>-</sup>, and SO<sub>4</sub><sup>2-</sup>) exceed unity, indicating that the soils are basic. Lead (Pb) isotope ratios of facility soils are similar to the AOSR stack and diesel emissions, while those of forest soils have much lower <sup>206</sup>Pb/<sup>207</sup>Pb and <sup>208</sup>Pb/<sup>207</sup>Pb ratios. High-molecular-weight <i>n</i>-alkanes (C<sub>25</sub>-C<sub>40</sub>), hopanes, and steranes are more than an order of magnitude more abundant in Facility than Forest profiles. These differences may be useful for separating anthropogenic from natural sources of fugitive dust at receptors.Implications: <i>Several organic compounds typical of combustion emissions and bitumen are enriched relative to forest soils for fugitive dust sources near oil sands operations, consistent with deposition uptake by biomonitors. AOSR dust samples are alkaline, not acidic, indicating that potential acid deposition is neutralized. Chemical abundances are highly variable within emission inventory categories, implying that more specific subcategories can be defined for inventory speciation.</i>
DOI: 10.6084/m9.figshare.1572130.v1
title: Characterization of PM<sub><b>2.5</b></sub> and PM<sub><b>10</b></sub> Fugitive Dust Source Profiles in the Athabasca Oil Sands Region

description: ABSTRACTGeological samples were collected from 27 representative locations in the Athabasca Oil Sands Region (AOSR) in Alberta, Canada. These samples were resuspended onto filter substrates for PM<sub>2.5</sub> and PM<sub>10</sub> size fractions. Samples were analyzed for 229 chemical species, consisting of elements, ions, carbon, and organic compounds. These chemical species are normalized to gravimetric mass to derive individual source profiles. Individual profiles were grouped into six categories typical of those used in emission inventories: paved road dust, unpaved road dust close to and distant from oil sand operations, overburden soil, tailings sands, and forest soils. Consistent with their geological origin, the major components are minerals, organic and elemental carbon, and ions. The sum of five major elements (i.e., Al, Si, K, Ca, and Fe) and their oxidized forms account for 25‒40% and 45‒82% of PM mass, respectively. Si is the most abundant element, averaging 17-18% in the Facility (oil sand operations) and 23-27% in the Forest profiles. Organic carbon is the second most abundant species, averaging 9-11% in the Facility and 5-6% in the Forest profiles. Elemental carbon abundance is 2‒3 times higher in Facility than Forest profiles. Sulfate abundance is ~7 times higher in the Facility than in the Forest profiles. The ratios of cation/anion and base cation (sum of Na<sup>+</sup>, Mg<sup>2+</sup>, K<sup>+</sup>, and Ca<sup>2+</sup>)/nitrogen- and sulfur-containing ions (sum of NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub>-, NO<sub>3</sub>-, and SO<sub>4</sub><sup>2-</sup>) exceed unity, indicating that the soils are basic. Lead (Pb) isotope ratios of facility soils are similar to the AOSR stack and diesel emissions, while those of forest soils have much lower <sup>206</sup>Pb/<sup>207</sup>Pb and <sup>208</sup>Pb/<sup>207</sup>Pb ratios. High molecular weight n-alkanes (C<sub>25</sub>-C<sub>40</sub>), hopanes, and steranes are over an order of magnitude more abundant in Facility than Forest profiles. These differences may be useful for separating anthropogenic from natural sources of fugitive dust at receptors.
DOI: 10.6084/m9.figshare.1574136
title: The M. hyorhinis p37 Protein Induces Cancer Associated Genes

description: 8 .CEL data files of the p37 Microarray analysis and 8 .XLSX data files of the qPCR analysis for the paper 'The Mycoplasma hyorhinis p37 Protein Rapidly Induces Genes in Fibroblasts Associated with Inflammation and Cancer'.
DOI: 10.6084/m9.figshare.1574136.v1
title: The M. hyorhinis p37 Protein Induces Cancer Associated Genes

description: 8 .CEL data files of the p37 Microarray analysis and 8 .XLSX data files of the qPCR analysis for the paper 'The Mycoplasma hyorhinis p37 Protein Rapidly Induces Genes in Fibroblasts Associated with Inflammation and Cancer'.
DOI: 10.6084/m9.figshare.1575699
title: COI-based barcoding of Chinese vipers (Reptilia: Squamata: Viperidae)

description: DNA barcoding seeks to assemble a standardized reference library for rapid and unambiguous identification of species, and can be used to screen for potentially cryptic species. The 5′ region of cytochrome oxidase subunit I (COI), which is a mitochondrial DNA (mtDNA) gene fragment, has been proposed as a universal marker for this purpose among animals. However, DNA barcoding of reptiles is still supported only by few datasets compared with other groups. We investigated theutilization of COI to discriminate 34 putative species of vipers, representing almost 92% of the recorded species in China.
Based on a total of 241 sequences, our results indicated that the average degree of intraspecific variability (0.0198) tends to be one-sixth the average of interspecific divergence (0.0931), but no barcoding gap was detected between them. The threshold method, BLOG analyses and tree-based methods all can identify species with a high success rate. These results consistently suggested the usefulness and reliability of the DNA barcoding approach in Chinese vipers.