cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7185425.v1
title: Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection

description: Abstract INTRODUCTION West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up to 500 days in certain patients. To evaluate the usefulness of immunoglobulin G (IgG) avidity assessment in the diagnosis of WNV infection, we analyzed 54 WNV IgM- and/or IgG-positive serum samples from 39 patients with neuroinvasive disease and 15 asymptomatic cases tested during a seroprevalence investigation. METHODS Serological tests (WNV IgM/IgG antibody detection, IgG avidity) were performed using commercially available enzyme-linked immunosorbent assays. RESULTS WNV IgM antibodies were detected in 47 (87%) samples. Acute/recent WNV infection was confirmed based on low/borderline avidity index (AI) in 44 IgM-positive samples (93.6%). In three IgM-positive samples (6.4%), high IgG AIs were detected, thus indicating persisting IgM antibodies from previous infections. All IgM-negative samples showed high AIs. Patients with WNV neuroinvasive disease tested within 30 days showed low AIs. In six patients tested 34-50 days after disease onset, AI was borderline (42%-60%), suggesting earlier WNV IgG maturation. Samples with the highest IgM values were associated with the lowest AIs (Spearman's rho coefficient -0.767, p &lt; 0.001). CONCLUSIONS Our results indicate that IgG avidity differentiates current/recent WNV infection from persistent IgM seropositivity from the previous WNV transmission season both in patients with WNV neuroinvasive disease and in asymptomatic persons. A strong negative correlation between IgM antibody levels and AI indicates that in cases with very high IgM levels, determination of IgG avidity may not be necessary. As many patients showed rapid avidity maturation, low IgG avidity is indicative of WNV infection within the previous month.
DOI: 10.6084/m9.figshare.7185413
title: In vitro and in vivo evaluation of sodium dodecyl sulfate (SDS) as an inactivator of caprine lentivirus (CLV) in colostrum and milk

description: ABSTRACT The aim of this study was to evaluate in vitro and in vivo the effect of sodium dodecyl sulfate (SDS) on the caprine lentivirus (CLV) in colostrum and milk. This was performed to develop a practical and efficient method of blocking the lactogenic transmission of the virus. In the in vitro experiment, colostrum and milk were treated with 0.25%; 0.50% and 1% SDS. Then, somatic cells of colostrum and milk were submitted to co-culture with caprine synovial membrane cells (CSM). In the in vivo test, goats were fed with colostrum and milk provided from CLV-positive goats treated with SDS in the same concentrations used in the in vitro experiment. Animals were tested by nested polymerase chain reaction (nPCR) and Western blot (WB) assays. In the in vitro experiment, inhibitory activity against CLV without inactivation occurred in colostrum with all SDS concentrations. However, concentrations of 0.25 and 0.5% SDS presented only inhibitory activity against CLV in milk cells, and 1% concentration provided inactivation of the virus. In the in vivo tests, none of the three concentrations of SDS was effective in inactivating LVC in colostrum or goat milk, which was confirmed by seroconversion and presence of proviral DNA in animals afterwards.
DOI: 10.6084/m9.figshare.7185419.v1
title: Experience of teacher education in graduate studies and research in education: Capes Pro-Ensino na Saude project, School of Medicine at the University of Sao Paulo, Ribeirao Preto, Brazil

description: This article describes the experience of the Capes Pro-Ensino na Saude project (Capes notice 024/2010) at the School of Medicine, University of Sao Paulo, Ribeirao Preto, Brazil. Between 2011 and 2016 the project provided the degrees of four masters and five doctors, in addition to creating subjects approaching topics on teaching-learning in health. In this period, there was the consolidation of the research group in health education, which has been developing specific projects and publishing original articles and reviews on subjects relevant to teaching in health. All objectives of the Capes notice were met, despite the difficulties faced such as little flexibility on the part of the graduate program in the university, which restricted the admission of graduate students and the accreditation of new advisors. The teaching and research group was kept even after the end of the project, and it still contributes to adding value to the teacher education in graduate studies and research in the health education area.
DOI: 10.6084/m9.figshare.7185413.v1
title: In vitro and in vivo evaluation of sodium dodecyl sulfate (SDS) as an inactivator of caprine lentivirus (CLV) in colostrum and milk

description: ABSTRACT The aim of this study was to evaluate in vitro and in vivo the effect of sodium dodecyl sulfate (SDS) on the caprine lentivirus (CLV) in colostrum and milk. This was performed to develop a practical and efficient method of blocking the lactogenic transmission of the virus. In the in vitro experiment, colostrum and milk were treated with 0.25%; 0.50% and 1% SDS. Then, somatic cells of colostrum and milk were submitted to co-culture with caprine synovial membrane cells (CSM). In the in vivo test, goats were fed with colostrum and milk provided from CLV-positive goats treated with SDS in the same concentrations used in the in vitro experiment. Animals were tested by nested polymerase chain reaction (nPCR) and Western blot (WB) assays. In the in vitro experiment, inhibitory activity against CLV without inactivation occurred in colostrum with all SDS concentrations. However, concentrations of 0.25 and 0.5% SDS presented only inhibitory activity against CLV in milk cells, and 1% concentration provided inactivation of the virus. In the in vivo tests, none of the three concentrations of SDS was effective in inactivating LVC in colostrum or goat milk, which was confirmed by seroconversion and presence of proviral DNA in animals afterwards.
DOI: 10.7284/119120
title: Gravimeter data collected during research cruise TN324 using a Bell BGM-3 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185407
title: COMPARISON OF THE POLLUTANT LEVELS EMITTED BY DIFFERENT FUELS USED FOR COOKING AND THEIR INFLUENCES ON GLOBAL WARMING

description: Global warming is one of the most worrying environmental problems nowadays. A joint effort has been made by many countries to minimize greenhouse gas (GHG) emissions, responsible for global warming. The evidences indicate that global warming is occurring due to anthropogenic activities, mainly caused by the using of fossil or biomass fuels. Although this effect is more directly associated with external emissions, domestic emissions also have their contributions. In this way, cooking activities can have an impact on global warming, since the main fuels used for these purposes are from fossil or biomass origin. This study aimed to evaluate the impact of the most common fuels used for cooking in Brazil on global warming. Among the fuels studied and the data available to date, biogas, followed by natural gas and liquefied petroleum gas were the least polluting, even the latter two being derived from petroleum and currently considered the great villain of air pollution. The results also show that although LPG is the most used fuel (&gt; 90%) in Brazil for cooking, firewood, which accounts for only 3% of consumption, causes the highest emission rates of greenhouse gases. Therefore, more efficient stoves can have a significant impact on the GHG reductions in the country.
DOI: 10.7284/121269
title: Gyrocompass data collected during research cruise TN324 using a Sperry MK-37 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185407.v1
title: COMPARISON OF THE POLLUTANT LEVELS EMITTED BY DIFFERENT FUELS USED FOR COOKING AND THEIR INFLUENCES ON GLOBAL WARMING

description: Global warming is one of the most worrying environmental problems nowadays. A joint effort has been made by many countries to minimize greenhouse gas (GHG) emissions, responsible for global warming. The evidences indicate that global warming is occurring due to anthropogenic activities, mainly caused by the using of fossil or biomass fuels. Although this effect is more directly associated with external emissions, domestic emissions also have their contributions. In this way, cooking activities can have an impact on global warming, since the main fuels used for these purposes are from fossil or biomass origin. This study aimed to evaluate the impact of the most common fuels used for cooking in Brazil on global warming. Among the fuels studied and the data available to date, biogas, followed by natural gas and liquefied petroleum gas were the least polluting, even the latter two being derived from petroleum and currently considered the great villain of air pollution. The results also show that although LPG is the most used fuel (&gt; 90%) in Brazil for cooking, firewood, which accounts for only 3% of consumption, causes the highest emission rates of greenhouse gases. Therefore, more efficient stoves can have a significant impact on the GHG reductions in the country.
DOI: 10.6084/m9.figshare.7185401
title: Chlorophyll a fluorescence in saccharine sorghum irrigated with saline water

description: ABSTRACT Soil salinity directly affects plants, interfering in the emission of fluorescence, and promoting the degradation of photosynthetic pigments. Thus, estimating the damage to the photosynthetic apparatus caused by saline water is an important tool to detect abiotic stresses. For this purpose, sorghum plants were cultivated in a greenhouse and irrigated with two water sources (NaCl and salt mixture) with six levels of electrical conductivity (EC) (0, 2.5, 5, 7.5, 10, and 12.5 dS m-1), forming a 6 × 2 factorial in randomized blocks with four repetitions. At 60 days after sowing, the photosynthetic pigments were quantified, the chlorophyll a fluorescence parameters (initial fluorescence [F0]; variable fluorescence [Fv]; maximum fluorescence [Fm]; quantum efficiency of photosystem II - PSII [Fv/Fm]; electron flux per reaction center [ET0/CR]; quantum energy dissipation [DI0/CR] and quantum yield for heat dissipation [φD0]) were evaluated. The photosynthetic pigments were decreased with an increase in the salinity of the irrigation water, and were more expressive at higher electrical conductivities. With the wear on the photosynthetic apparatus by the increase in salinity, a reduction in the emission of the chlorophyll a fluorescence was observed, pointing to a possible photoinhibition of photosystem II.
DOI: 10.7284/121095
title: Meteorological Station data collected during research cruise TN324 using a RM Young 26800 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185392
title: Water potential and gas exchanges in sugarcane irrigated with saline waters

description: ABSTRACT In Pernambuco, state in the northeastern region of Brazil, in the coastal areas, due to the intrusion of seawater, the waters used for sugarcane irrigation can have high salt contents and cause serious problems to soil and plant. The present study aimed to evaluate the effects of irrigation with saline water on the physiology of sugarcane, variety RB867515, irrigated under five salinity levels of 0.5; 2.0; 3.5; 5.0 and 6.5 dS m-1 in a completely randomized design, with four replicates in drainage lysimeters. The study was conducted in the period from December 2014 to June 2015, at the Federal Rural University of Pernambuco (UFRPE). Salinity levels were obtained by dissolving NaCl and CaCl2 in water from the local supply system (ECw = 0.5 dS m-1). Leaves were analyzed for stomatal conductance, transpiration and photosynthesis at 140, 229 and 320 days after planting (DAP) and for water potential at 137, 243 and 318 DAP. Increase in irrigation water salinity inhibited all variables at the respective plant ages and with greater intensity in the first evaluations (140 and 229 DAP) for stomatal conductance and transpiration. Photosynthesis and water potential showed the greatest linear reductions at the last data collection (320 and 318 DAP), respectively. Increase in salinity of irrigation water hampered water potential and gas exchange in the leaves of RB867515 sugarcane.
DOI: 10.6084/m9.figshare.7185395
title: Genetic Diversity of Mitochondrial DNA of Chinese Black-bone Chicken

description: ABSTRACT The black-bone chicken has special economic value in Chinese poultry breeds, which also are valued for the medicinal properties of their meat in traditional Chinese medicine. In order to protect the genetic resources of native black-bone chicken breeds, we analyzed the genetic diversity and matrilineal components of 64 mtDNA D-loop partial sequences from three native black-bone chicken breeds, together with reported 596 black-bone chicken mtDNA sequences from China, Japan, and Korea. A total of 108 haplotypes were observed from 73 variable sites. These domestic chicken mtDNA sequences could be assigned into seven clades (A-G). The results indicated that 71.97% of the black-bone haplotypes were related to the reference sequence that may originate from Eurasia, while the minor part of mtDNA sequences presumably derive from Southeast Asia, China, and Japan. Three clades were shared by Korean, Japanese, and Chinese black-bone chickens. These results provide basic data useful for making new breeding and conservation strategies for the black-bone chicken in China.
DOI: 10.6084/m9.figshare.7185401.v1
title: Chlorophyll a fluorescence in saccharine sorghum irrigated with saline water

description: ABSTRACT Soil salinity directly affects plants, interfering in the emission of fluorescence, and promoting the degradation of photosynthetic pigments. Thus, estimating the damage to the photosynthetic apparatus caused by saline water is an important tool to detect abiotic stresses. For this purpose, sorghum plants were cultivated in a greenhouse and irrigated with two water sources (NaCl and salt mixture) with six levels of electrical conductivity (EC) (0, 2.5, 5, 7.5, 10, and 12.5 dS m-1), forming a 6 × 2 factorial in randomized blocks with four repetitions. At 60 days after sowing, the photosynthetic pigments were quantified, the chlorophyll a fluorescence parameters (initial fluorescence [F0]; variable fluorescence [Fv]; maximum fluorescence [Fm]; quantum efficiency of photosystem II - PSII [Fv/Fm]; electron flux per reaction center [ET0/CR]; quantum energy dissipation [DI0/CR] and quantum yield for heat dissipation [φD0]) were evaluated. The photosynthetic pigments were decreased with an increase in the salinity of the irrigation water, and were more expressive at higher electrical conductivities. With the wear on the photosynthetic apparatus by the increase in salinity, a reduction in the emission of the chlorophyll a fluorescence was observed, pointing to a possible photoinhibition of photosystem II.
DOI: 10.6084/m9.figshare.7185395.v1
title: Genetic Diversity of Mitochondrial DNA of Chinese Black-bone Chicken

description: ABSTRACT The black-bone chicken has special economic value in Chinese poultry breeds, which also are valued for the medicinal properties of their meat in traditional Chinese medicine. In order to protect the genetic resources of native black-bone chicken breeds, we analyzed the genetic diversity and matrilineal components of 64 mtDNA D-loop partial sequences from three native black-bone chicken breeds, together with reported 596 black-bone chicken mtDNA sequences from China, Japan, and Korea. A total of 108 haplotypes were observed from 73 variable sites. These domestic chicken mtDNA sequences could be assigned into seven clades (A-G). The results indicated that 71.97% of the black-bone haplotypes were related to the reference sequence that may originate from Eurasia, while the minor part of mtDNA sequences presumably derive from Southeast Asia, China, and Japan. Three clades were shared by Korean, Japanese, and Chinese black-bone chickens. These results provide basic data useful for making new breeding and conservation strategies for the black-bone chicken in China.
DOI: 10.7284/119119
title: Multibeam Echosounder data collected during research cruise TN324 using a Kongsberg EM302 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185392.v1
title: Water potential and gas exchanges in sugarcane irrigated with saline waters

description: ABSTRACT In Pernambuco, state in the northeastern region of Brazil, in the coastal areas, due to the intrusion of seawater, the waters used for sugarcane irrigation can have high salt contents and cause serious problems to soil and plant. The present study aimed to evaluate the effects of irrigation with saline water on the physiology of sugarcane, variety RB867515, irrigated under five salinity levels of 0.5; 2.0; 3.5; 5.0 and 6.5 dS m-1 in a completely randomized design, with four replicates in drainage lysimeters. The study was conducted in the period from December 2014 to June 2015, at the Federal Rural University of Pernambuco (UFRPE). Salinity levels were obtained by dissolving NaCl and CaCl2 in water from the local supply system (ECw = 0.5 dS m-1). Leaves were analyzed for stomatal conductance, transpiration and photosynthesis at 140, 229 and 320 days after planting (DAP) and for water potential at 137, 243 and 318 DAP. Increase in irrigation water salinity inhibited all variables at the respective plant ages and with greater intensity in the first evaluations (140 and 229 DAP) for stomatal conductance and transpiration. Photosynthesis and water potential showed the greatest linear reductions at the last data collection (320 and 318 DAP), respectively. Increase in salinity of irrigation water hampered water potential and gas exchange in the leaves of RB867515 sugarcane.
DOI: 10.7284/121097
title: Radiometer data collected during research cruise TN324 using a Biospherical QSR-2150 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185380
title: Characterization of Pantoea ananatis isolates and reaction of maize genotypes to the bacterium

description: ABSTRACT Maize white leaf spot, caused by the bacterium Pantoea ananatis, is one of the main leaf diseases affecting this crop. Using varieties resistant to the bacterium is the best strategy for the disease management. Thus, the aims of this study were to characterize and identify Pantoea ananatis isolates and evaluate maize hybrids resistant to this bacterial disease. The bacterium was isolated from maize leaves showing symptoms of water-soaked lesions. The bacterial isolates UFU A18 (Morrinhos-GO) and UFU B13 (Planaltina-GO) were identified based on cultural, biochemical and molecular characteristics, and pathogenicity in maize plants. The maize hybrids encoded as A1, A2, B1, B2 and B3 were inoculated with two bacterial isolates. The experiment was conducted in a greenhouse in randomized block design and factorial scheme (2 isolates x 5 hybrids) with six replicates. Disease severity was calculated on four evaluations, using a rating scale ranging from 0 to 4 and calculating the area under the severity progress curve. Bacterial isolates were identified as Pantoea ananatis and the maize hybrid A1 was resistant to the bacterium when inoculated with the bacterial isolate UFU B13.
DOI: 10.6084/m9.figshare.7185383
title: Physical performance of particleboards using Castor oil-based adhesive

description: ABSTRACT Reconstituted wood panels are widely used in various branches of the wood industry, such as in the furniture sectors and various segments of urban and rural construction. In Brazil, agricultural and forestry residues are abundantly generated, making feasible the study, development and application of alternative and sustainable materials, produced from the use of these wastes. The aim of this study was to produce high-density homogenous panels with wood residues of Pinus elliottii and oat hulls (Avena sativa), pressed with two types of adhesive, castor oil-based polyurethane and melamine formaldehyde, and to evaluate the physical performance of these panels. The physical performance of the panels was evaluated based on the ABNT NBR 14810: 2006, ANSI A208.1: 1999 and BS EN 312: 2003 standards. Analysis of variance (ANOVA) was performed to test the influence of the factors percentage of oat hull, percentage of adhesive and type of adhesive, and the interactions between these factors on the physical properties of the panels. The results indicated physical properties, in some treatments superior to the requirements stipulated by the consulted standards. It was concluded that the panels produced with the castor oil-based polyurethane adhesive showed better physical performance when compared with the panels produced with the melamine formaldehyde adhesive.
DOI: 10.6084/m9.figshare.7185380.v1
title: Characterization of Pantoea ananatis isolates and reaction of maize genotypes to the bacterium

description: ABSTRACT Maize white leaf spot, caused by the bacterium Pantoea ananatis, is one of the main leaf diseases affecting this crop. Using varieties resistant to the bacterium is the best strategy for the disease management. Thus, the aims of this study were to characterize and identify Pantoea ananatis isolates and evaluate maize hybrids resistant to this bacterial disease. The bacterium was isolated from maize leaves showing symptoms of water-soaked lesions. The bacterial isolates UFU A18 (Morrinhos-GO) and UFU B13 (Planaltina-GO) were identified based on cultural, biochemical and molecular characteristics, and pathogenicity in maize plants. The maize hybrids encoded as A1, A2, B1, B2 and B3 were inoculated with two bacterial isolates. The experiment was conducted in a greenhouse in randomized block design and factorial scheme (2 isolates x 5 hybrids) with six replicates. Disease severity was calculated on four evaluations, using a rating scale ranging from 0 to 4 and calculating the area under the severity progress curve. Bacterial isolates were identified as Pantoea ananatis and the maize hybrid A1 was resistant to the bacterium when inoculated with the bacterial isolate UFU B13.
DOI: 10.7284/121098
title: Radiometer data collected during research cruise TN324 using a Eppley RAD instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition