cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7186637
title: Oxidative stress in dogs with periodontal disease: comparison of plasmatic and salivary biomarkers&gt;

description: ABSTRACT Periodontal disease (PD) is the most common inflammatory disease of the oral cavity of dogs. Quantitation of plasma and salivary biomarkers have been used to assess the systemic oxidative stress (SOS) and local (LOS) of human PD. In canine PD, oxidative stress mechanisms are not well characterized and established. Our objective was to investigate the hypothesis that SOS occurs in dog PD and saliva can be used to evaluate the LOS. We also investigated the hypothesis that the activation of neutrophil oxidative metabolism contributes to SOS in dog SD. For this purpose, 20 adult dogs were selected PD patients, grouped according to the degree of injury: gingivitis (n=6), light periodontitis (n=8) and advanced periodontitis (n=6). The control group was composed of the same 20 dogs, 30 days after periodontal treatment. To assess oxidative metabolism of circulating neutrophils superoxide production was measured by test nitroblue tetrazolium reduction (NBT). The total oxidant concentrations (TOC) and reactive species to tiobartbitúrico acid (TBARS) in plasma were quantified to evaluate SOS. For the evaluation of local oxidative stress were quantified salivary TOC and concentration of the main antioxidant in saliva (albumin, uric acid, and total bilirubin). EOS in dogs with PD was confirmed by increased superoxide production of circulating neutrophils, TOC, and plasma TBARS. It was possible to quantify all the biomarkers in the saliva of dogs, but none was able to express the LOS canine PD. This is the first evidence that SOS occurs in dogs with PD and that activation of the oxidative metabolism of neutrophils may contribute to an imbalance between oxidants and antioxidants. This study highlights the importance of oral hygiene of dogs to prevent PD and chronic degenerative lesions of various tissues caused by SOS.
DOI: 10.6084/m9.figshare.7186634.v1
title: Multiple-Injection Capillary Zone Electrophoresis as a Fast Strategy to Determine Antinitrosating Capacity of Commercial Teas

description: This paper describes the use of a multiple-injection capillary electrophoresis method as a fast strategy to determine the antinitrosating capacity of nine commercial teas through nitrite quantification. The method consists of the injection of the sample followed by the injection of the control solution, employing a fused-silica capillary of 32.0 cm total length (23.5 cm effective length, 50 µm internal diameter) with background electrolyte composed of 4.0 g L-1 β-alanine and 1.5 g L-1 perchloric acid (pH 3.79) and sodium thiocyanate was used as the internal standard. Before the injections the tea samples were maintained by 1 h of incubation, at 37 °C, with sodium nitrite in perchloric acid medium (pH 2.3). In order to avoid nitrite oxidation and nitrate formation, ultra-pure nearly oxygen-free water was used to prepare the solutions. Black tea, green tea and white tea, obtained from Camellia sinensis, showed greater antinitrosating capacity (96, 93 and 89%, respectively).
DOI: 10.6084/m9.figshare.7186637.v1
title: Oxidative stress in dogs with periodontal disease: comparison of plasmatic and salivary biomarkers&gt;

description: ABSTRACT Periodontal disease (PD) is the most common inflammatory disease of the oral cavity of dogs. Quantitation of plasma and salivary biomarkers have been used to assess the systemic oxidative stress (SOS) and local (LOS) of human PD. In canine PD, oxidative stress mechanisms are not well characterized and established. Our objective was to investigate the hypothesis that SOS occurs in dog PD and saliva can be used to evaluate the LOS. We also investigated the hypothesis that the activation of neutrophil oxidative metabolism contributes to SOS in dog SD. For this purpose, 20 adult dogs were selected PD patients, grouped according to the degree of injury: gingivitis (n=6), light periodontitis (n=8) and advanced periodontitis (n=6). The control group was composed of the same 20 dogs, 30 days after periodontal treatment. To assess oxidative metabolism of circulating neutrophils superoxide production was measured by test nitroblue tetrazolium reduction (NBT). The total oxidant concentrations (TOC) and reactive species to tiobartbitúrico acid (TBARS) in plasma were quantified to evaluate SOS. For the evaluation of local oxidative stress were quantified salivary TOC and concentration of the main antioxidant in saliva (albumin, uric acid, and total bilirubin). EOS in dogs with PD was confirmed by increased superoxide production of circulating neutrophils, TOC, and plasma TBARS. It was possible to quantify all the biomarkers in the saliva of dogs, but none was able to express the LOS canine PD. This is the first evidence that SOS occurs in dogs with PD and that activation of the oxidative metabolism of neutrophils may contribute to an imbalance between oxidants and antioxidants. This study highlights the importance of oral hygiene of dogs to prevent PD and chronic degenerative lesions of various tissues caused by SOS.
DOI: 10.6084/m9.figshare.7186628
title: SYNTHESIS OF GOLD NANOPARTICLES BY TETRACHLOROAURATE REDUCTION WITH CYCLODEXTRINS

description: One-step synthesis of gold nanoparticles functionalized with α-, β- and γ-cyclodextrins was carried out using a synthetic method of direct tetrachloroaurate reduction in alkaline solutions. The two experimental conditions studied were direct heating and microwave irradiation. This is the first publication comparing the characteristics of gold nanoparticles produced using three cyclodextrins under different conditions. The resulting nanoparticles were characterized by absorbance spectra, FTIR spectra, dynamic light scattering data and transmission electron microscopy images. The reducing ability of cyclodextrins increased in the following order: β-cyclodextrin &lt; α-cyclodextrin &lt; γ-cyclodextrin. Therefore, γ-cyclodextrin was the most active reductant and the only cyclodextrin that was able to reduce tetrachloroaurate at room temperature. In this case, nanoparticles were observed only after 4 days of storage. The nanoparticle size was dependent on the cyclodextrin type and correlated with the difference in the reducing ability. Using microwave irradiation, most of nanoparticles were found to be free nanoparticles. Using direct heating, aggregates formed due to the high ionic strength of the solution after prolonged boiling. Therefore, nanoparticles synthesized by direct heating were less stable during storage than those synthesized by microwave irradiation.
DOI: 10.6084/m9.figshare.7186631
title: Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation

description: An investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The method of salting-out assisted liquid-liquid extraction (SALLE), using three different NaCl concentration levels, obtained recoveries in the range of 47.4-103.4% at pH 5.0 and 36.6-106.8% at pH 7.0, with an intra-day relative standard deviation under 15% for the majority of the compounds. Quantification limits remained in the region of 0.07 µg mL-1 for 15-acetyldeoxynivalenol and 0.3 µg mL-1 for nivalenol. Finally, the suitable analytical methods were applied in a study of trichothecene degradation by enzymatic action, resulting in a reduction of 12.3% for T-2 toxin, 68.4% for deoxynivalenol, 50.2% for 3-acetyldeoxynivalenol and 45.4% for 15-acetyldeoxynivalenol.
DOI: 10.7284/121199
title: Gyrocompass data collected during research cruise TN310 using a Sperry MK-37 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186631.v1
title: Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation

description: An investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The method of salting-out assisted liquid-liquid extraction (SALLE), using three different NaCl concentration levels, obtained recoveries in the range of 47.4-103.4% at pH 5.0 and 36.6-106.8% at pH 7.0, with an intra-day relative standard deviation under 15% for the majority of the compounds. Quantification limits remained in the region of 0.07 µg mL-1 for 15-acetyldeoxynivalenol and 0.3 µg mL-1 for nivalenol. Finally, the suitable analytical methods were applied in a study of trichothecene degradation by enzymatic action, resulting in a reduction of 12.3% for T-2 toxin, 68.4% for deoxynivalenol, 50.2% for 3-acetyldeoxynivalenol and 45.4% for 15-acetyldeoxynivalenol.
DOI: 10.6084/m9.figshare.7186625
title: Diagrammatic scale for powdery mildew severity evaluation in cashew nuts.

description: ABSTRACT Powdery mildew, caused by the fungus Pseudoidium anacardii, is currently the main disease of cashew tree (Anacardium occidentale). In view of the importance of such plant disease and the absence of standardized methods for its quantification, a descriptive diagrammatic scale was proposed with levels 0 (Score 0), 5 (Score 1), 17.5 (Score 2), 37.5 (Score 3), 62.5 (Score 4) and 87.5% injured superficial area (Score 5), and was elaborated and tested for accuracy, precision and reproducibility of severity estimates of fungal attack on cashew nuts. The scale was tested by twenty people (ten experienced and ten inexperienced people) who used fifty nuts showing different severity levels, previously measured with the Assess® program, to be evaluated without and with the proposed descriptive diagrammatic scale. Simple linear regressions between the actual severity (measured electronically) and the severity estimated by each evaluator were used to determine accuracy and precision. Reproducibility was obtained based on linear regressions between severities of the same sampling unit estimated by different raters, combined in pairs, with and without the scale. The descriptive diagrammatic scale to assess powdery mildew severity in cashew nuts was easy to use and improved the levels of accuracy, precision and reproducibility of the estimates, regardless of the evaluator’s experience. Using the scale in field surveys and epidemiological, resistance and powdery mildew control measures studies will provide more precise information on the disease intensity in such an important pathosystem, besides allowing its use by students, technicians, researchers and farmers to quantify powdery mildew symptoms in cashew plants.
DOI: 10.6084/m9.figshare.7186628.v1
title: SYNTHESIS OF GOLD NANOPARTICLES BY TETRACHLOROAURATE REDUCTION WITH CYCLODEXTRINS

description: One-step synthesis of gold nanoparticles functionalized with α-, β- and γ-cyclodextrins was carried out using a synthetic method of direct tetrachloroaurate reduction in alkaline solutions. The two experimental conditions studied were direct heating and microwave irradiation. This is the first publication comparing the characteristics of gold nanoparticles produced using three cyclodextrins under different conditions. The resulting nanoparticles were characterized by absorbance spectra, FTIR spectra, dynamic light scattering data and transmission electron microscopy images. The reducing ability of cyclodextrins increased in the following order: β-cyclodextrin &lt; α-cyclodextrin &lt; γ-cyclodextrin. Therefore, γ-cyclodextrin was the most active reductant and the only cyclodextrin that was able to reduce tetrachloroaurate at room temperature. In this case, nanoparticles were observed only after 4 days of storage. The nanoparticle size was dependent on the cyclodextrin type and correlated with the difference in the reducing ability. Using microwave irradiation, most of nanoparticles were found to be free nanoparticles. Using direct heating, aggregates formed due to the high ionic strength of the solution after prolonged boiling. Therefore, nanoparticles synthesized by direct heating were less stable during storage than those synthesized by microwave irradiation.
DOI: 10.6084/m9.figshare.7186622
title: Calibration of a soil moisture sensor with disturbed and undisturbed soil samples from Bahia

description: ABSTRACT The objective of this study was to evaluate the models proposed by manufacturers and in the literature with respect to soil moisture measurement and to evaluate the performance of the CS616 sensor in the calibration of disturbed and undisturbed soil samples. These calibrations were performed using linear and quadratic models. Disturbed samples were collected in São Gabriel/BA, six samples placed in pots, whereas undisturbed samples were collected in Cruz das Almas/BA, three samples directly collected in the area and placed in a container. A calibration was performed between 21/12/2016 and 08/01/2017. The models proposed in the literature and by manufacturers differed in the estimation of volumetric soil moisture. Disturbed soil samples had higher data dispersion than undisturbed samples, due to factors such as grain size and bulk density, which influence the calibration data. The CS616 sensor had satisfactory performance in the calibration of disturbed and undisturbed samples, with excellent fit of the soil moisture data. Using soil moisture contents obtained by the CS616 sensor, without a previous calibration, may lead to errors in the results, confirming the need for a specific calibration for each type of soil.
DOI: 10.6084/m9.figshare.7186625.v1
title: Diagrammatic scale for powdery mildew severity evaluation in cashew nuts.

description: ABSTRACT Powdery mildew, caused by the fungus Pseudoidium anacardii, is currently the main disease of cashew tree (Anacardium occidentale). In view of the importance of such plant disease and the absence of standardized methods for its quantification, a descriptive diagrammatic scale was proposed with levels 0 (Score 0), 5 (Score 1), 17.5 (Score 2), 37.5 (Score 3), 62.5 (Score 4) and 87.5% injured superficial area (Score 5), and was elaborated and tested for accuracy, precision and reproducibility of severity estimates of fungal attack on cashew nuts. The scale was tested by twenty people (ten experienced and ten inexperienced people) who used fifty nuts showing different severity levels, previously measured with the Assess® program, to be evaluated without and with the proposed descriptive diagrammatic scale. Simple linear regressions between the actual severity (measured electronically) and the severity estimated by each evaluator were used to determine accuracy and precision. Reproducibility was obtained based on linear regressions between severities of the same sampling unit estimated by different raters, combined in pairs, with and without the scale. The descriptive diagrammatic scale to assess powdery mildew severity in cashew nuts was easy to use and improved the levels of accuracy, precision and reproducibility of the estimates, regardless of the evaluator’s experience. Using the scale in field surveys and epidemiological, resistance and powdery mildew control measures studies will provide more precise information on the disease intensity in such an important pathosystem, besides allowing its use by students, technicians, researchers and farmers to quantify powdery mildew symptoms in cashew plants.
DOI: 10.7284/121038
title: Meteorological Station data collected during research cruise TN310 using a RM Young 26800 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186619
title: Self-efficacy sources and experimental physics activities: an exploratory study

description: Abstract Part of the Physics Education Research (PER) community has devoted its attention to the study of Active and Flipped Learning methods. Beyond learning, these methods seek to foster the development of collaborative work and argumentation skill, and also to decrease student's dropout rates. In experimental physics courses, some of those methods involve the scientific modeling learning (e.g., Modeling Instruction (MI) and Modeling Episodes (ME)). However, the success of such initiatives depends on how students judge themselves capable of carrying out the activities proposed by the teacher, that is, it depends on the student's self-efficacy in perform certain actions. Therefore, we propose a case-study with the purpose of analyze the impact of the ME on the students' self-efficacy beliefs about learning physics; conduct experimental activities; and work collaboratively. The results indicate positive changes in students' self-efficacy due to positive experiences, observation of the classmates, and teacher feedback.
DOI: 10.6084/m9.figshare.7186622.v1
title: Calibration of a soil moisture sensor with disturbed and undisturbed soil samples from Bahia

description: ABSTRACT The objective of this study was to evaluate the models proposed by manufacturers and in the literature with respect to soil moisture measurement and to evaluate the performance of the CS616 sensor in the calibration of disturbed and undisturbed soil samples. These calibrations were performed using linear and quadratic models. Disturbed samples were collected in São Gabriel/BA, six samples placed in pots, whereas undisturbed samples were collected in Cruz das Almas/BA, three samples directly collected in the area and placed in a container. A calibration was performed between 21/12/2016 and 08/01/2017. The models proposed in the literature and by manufacturers differed in the estimation of volumetric soil moisture. Disturbed soil samples had higher data dispersion than undisturbed samples, due to factors such as grain size and bulk density, which influence the calibration data. The CS616 sensor had satisfactory performance in the calibration of disturbed and undisturbed samples, with excellent fit of the soil moisture data. Using soil moisture contents obtained by the CS616 sensor, without a previous calibration, may lead to errors in the results, confirming the need for a specific calibration for each type of soil.
DOI: 10.6084/m9.figshare.7186616
title: Physiological responses of anti-predation in prey fish to the threat of piscivorous fish in different underwater visibility conditions

description: ABSTRACT Considering that the anti-predation behaviour of prey fishes may vary with predator hunting strategy, we experimentally investigated the physiological responses of Astyanax bimaculatus (Linnaeus, 1758) shoals to the presence of two piscivorous fish species with different hunting modes: sit-and-wait and active pursuit. In addition, we evaluated the influence of underwater visibility conditions on the interaction between predator and prey. We tested the hypotheses that: (i) prey plasma cortisol and glucose levels vary according to piscivore hunting strategy, and (ii) no reduction in plasma glucose and cortisol levels takes place in turbid environments due to the inability of prey to accurately recognise predators by non-visual signals. The results revealed that the presence of piscivorous fish increased plasma cortisol levels in prey, with no significant difference recorded between the two hunting strategy treatments. We also observed no significant change in plasma glucose levels in different water transparency conditions. Thus, we conclude that physiological changes in the selected prey fish do not vary with predator hunting mode, and it is therefore necessary to consider the ability of the prey to recognise and evaluate danger, regardless of piscivore hunting strategy.
DOI: 10.6084/m9.figshare.7186619.v1
title: Self-efficacy sources and experimental physics activities: an exploratory study

description: Abstract Part of the Physics Education Research (PER) community has devoted its attention to the study of Active and Flipped Learning methods. Beyond learning, these methods seek to foster the development of collaborative work and argumentation skill, and also to decrease student's dropout rates. In experimental physics courses, some of those methods involve the scientific modeling learning (e.g., Modeling Instruction (MI) and Modeling Episodes (ME)). However, the success of such initiatives depends on how students judge themselves capable of carrying out the activities proposed by the teacher, that is, it depends on the student's self-efficacy in perform certain actions. Therefore, we propose a case-study with the purpose of analyze the impact of the ME on the students' self-efficacy beliefs about learning physics; conduct experimental activities; and work collaboratively. The results indicate positive changes in students' self-efficacy due to positive experiences, observation of the classmates, and teacher feedback.
DOI: 10.7284/121040
title: Radiometer data collected during research cruise TN310 using a Biospherical QSR-2150 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7186616.v1
title: Physiological responses of anti-predation in prey fish to the threat of piscivorous fish in different underwater visibility conditions

description: ABSTRACT Considering that the anti-predation behaviour of prey fishes may vary with predator hunting strategy, we experimentally investigated the physiological responses of Astyanax bimaculatus (Linnaeus, 1758) shoals to the presence of two piscivorous fish species with different hunting modes: sit-and-wait and active pursuit. In addition, we evaluated the influence of underwater visibility conditions on the interaction between predator and prey. We tested the hypotheses that: (i) prey plasma cortisol and glucose levels vary according to piscivore hunting strategy, and (ii) no reduction in plasma glucose and cortisol levels takes place in turbid environments due to the inability of prey to accurately recognise predators by non-visual signals. The results revealed that the presence of piscivorous fish increased plasma cortisol levels in prey, with no significant difference recorded between the two hunting strategy treatments. We also observed no significant change in plasma glucose levels in different water transparency conditions. Thus, we conclude that physiological changes in the selected prey fish do not vary with predator hunting mode, and it is therefore necessary to consider the ability of the prey to recognise and evaluate danger, regardless of piscivore hunting strategy.