DOI: 10.5061/dryad.djh9w0w3g
title: Responsiveness of the broad bean weevil Bruchus rufimanus Boh. to different Vicia faba L. genotypes

description: The broad bean weevil Bruchus rufimanus Boh., also called bruchid beetle,
 is a major pest of Vicia faba L. given that larvae develop inside the
 seeds causing direct quality losses in agricultural products. Recurrent
 chemical applications are inappropriate because they represent a serious
 threat to pollinators in general. In addition, biological control
 approaches that have been attempted so far showed limited effectiveness,
 which makes studies of methods to control bruchid populations extremely
 important. Breeding resistant cultivars is the most adequate approach to
 achieve efficient levels of pest resistance and promote sustainable
 agriculture. To explore the mechanisms underlying bruchid resistance, we
 explored B. rufimanus behavior in different life stages and plant
 preference in feeding, oviposition and larval survival into adulthood both
 in greenhouse experiments and in the field. Our greenhouse results showed
 that females, compared to males, have preferences for flowers from some
 genotypes over the others although the choices made did not correspond
 with the laying preference. The egg laying preference of females was
 significantly affected by cultivars. This attraction could be explained by
 the number of seeds per pod produced by the genotypes, while in the field
 flowering time influenced bruchid infestation. Moreover, the survival of
 the larva into adulthood was greater in certain genotypes over the others.
 The results of our study lay the groundwork for further analyses to finely
 dissect V. faba resistance towards bruchids and pave the way for the
 development of methods to predict potential resistant genotypes in
 research and breeding programs.
description: 1. Description of methods used for collection/generation of
 data: Behaviour data collected from live B. rufimanus
 adults under controlled conditions. Plant phenological
 traits collected in the field. Seed infestation data
 collect in laboratory conditions. 2. Methods for
 processing the data: All data collected was handled
 using R software.
description: R software
description: ## README This readme file was generated on 12th September 2022 by Denise
 Dalbosco Dell'Aglio ## GENERAL INFORMATION Title: Responsiveness of
 the broad bean weevil Bruchus rufimanus Boh. to different Vicia faba L.
 genotypes Authors: Denise Dalbosco Dell'Aglio Nadim Tayeh
 Affiliation: Agrocologie, INRAE, Institut Agro, Univ. Bourgogne
 Franche-Comt, 21000, Dijon, France Corresponding author: Denise Dalbosco
 Dell'Aglio email:
 [denise.ddd3@gmail.com](mailto:denise.ddd3@gmail.com) ## DATA & FILE
 OVERVIEW File dataset list: Ovip_16July2021.txt
 Ovip_4dates_5accessions.txt data_field_16march2022.txt
 2Choices_July2021.txt Adult_18Jan2022.txt 1Choice_July2021.txt
 2choices_bysex.txt File R script: script_bruchid_manusc.R ##
 METHODOLOGICAL INFORMATION Description of methods used for
 collection/generation of data: Behaviour data collected from live B.
 rufimanus adults under controlled conditions. Plant phenological traits
 collected in the field. Seed infestation data collect in laboratory
 conditions. Methods for processing the data: All data collected was
 handled using R software. ## DATA SPECIFIC INFORMATION The data files
 contains all necessary to be upload into R environment. The R script
 contains the statistical models, together with each data file to be used
 and graphs created for the manuscript. More specific details in the R
 script. **Abbreviations and Variable descriptions:**
 File Ovip_16July2021.txt: "Name" (name of the Faba
 type), "Rep" (replica), "Date" (dd/mm/yyyy),
 "Branch" (number of the plant branch), "Pod.date"
 (date that the pod start growing, dd/mm/yyyy), "Pod.age" (number
 of days since Pod.date), "N.eggs" (number of
 eggs), "New" (if the eggs are new, n for yes)
 File Ovip_4dates_5accessions: "Name" (name of the Faba
 type), "Rep" (replica), "Date" (dd/mm/yyyy),
 "Branch" (number of the plant branch), "Pod.date"
 (date that the pod start growing, dd/mm/yyyy), "Pod.age" (number
 of days since Pod.date), "N.eggs" (number of
 eggs), "New" (if the eggs are new, n for yes) File
 data_field_16march2022.txt: "GPID" (genotype of Faba bean),
 "Rep" (replica), "Flw_time" (Floweration time in
 days), "Plant_hm" (Plant height mean in cm),
 "Seed_100w" (weight of 100 seeds in g), "Seeds_htyp"
 (percentage of healthy seeds) File 2Choices_July2021.txt:
 "choice.name" (name of the choice pair), "choice.A"
 (name of first choice), "choice.B" (name of second choice),
 "sex" (if male or female), "status" (naive, never fed
 before; mature, already fed; all, both naive and mature data together),
 "ind" (number of individuals), "result.A" (number of
 individuals which choose the choice A),  "result.B" (number of
 individuals which choose the choice B), "prop.A" (proportion of
 choice A), "prop.B" (proportion of choice B)
 File Adult_18Jan2022.txt: "name" (name of faba genotype),
 "rep" (replica), "branch" (number of the plant
 branch), "pod_date" (date that the pod start growing,
 dd/mm/yyyy), "pod_age" (number of days since
 Pod.date), "eggs_n" (number of eggs), "seeds_n" (total
 number of seeds), "infested_n" (number of infested seeds),
 "holes_n" (total number of holes in the seeds),
 "seeds_weight" (total weight of the seeds in g),
 "holes_seed" (number of holes per seed),
 "infested_per" (percentage of infested seeds),
 "oneseed_weight" (the weight of 1 seed), "eggs_seed"
 (number of eggs per seed) File
 1Choice_July2021.txt: "choice.name" (name of the choice pair),
 "choice.A" (name of first choice), "choice.B" (name of
 second choice), "sex" (if male or female), "status"
 (naive, never fed before; mature, already fed; all, both naive and mature
 data together), "n_ind" (number of individuals),
 "result.A" (number of individuals which choose the choice A), 
 "result.B" (number of individuals which choose the choice B),
 "total" (total number of individuals which made a choice),
 "prop.A" (proportion of choice A), "prop.B"
 (proportion of choice B), "signf" (if the difference between A
 and B is statistically significant, y for yes, n for no) File
 2choices_bysex.txt: "choice.name" (name of the choice
 pair), "GPID" (genotype of Faba bean), "sex" (if male
 or female), "n_ind" (number of individuals which made the
 choice), "prop" (proportion of individuals which made a choice)