DOI: 10.5061/dryad.3xsj3txw1
title: Data from: Organ-specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

description: This dataset supports the manuscript “Organ-specific redox imbalance in
 spinal muscular atrophy mice and partial rescue by SMN antisense
 oligonucleotides” submitted to FEBS Letters. It contains raw and processed
 experimental data generated to investigate tissue-specific redox
 alterations and lipid peroxidation in a spinal muscular atrophy (SMA)
 mouse model and following treatment with SMN antisense oligonucleotides.
 The dataset includes uncropped raw Western blot images corresponding to
 all main and supplementary figures, quantitative values used for graphical
 representation and statistical analysis, and raw and processed lipid
 peroxidation (MDA/TBARS) measurements obtained using a TECAN plate reader.
 Processed data include blank correction, standard-curve-based conversion
 to MDA equivalents, normalization to protein content, dilution correction,
 and final normalization as used for figure generation in GraphPad Prism.
 All files are organized by figure and assay type, with detailed README
 documentation and processing notes provided to enable full reproducibility
 and reuse. This dataset may be reused for independent reanalysis,
 methodological comparison, or meta-analyses of redox regulation and
 oxidative stress in neuromuscular disease models.
description: # Data from: Organ-specific redox imbalances in spinal muscular atrophy
 mice are partially rescued by SMN antisense oligonucleotides
 [https://doi.org/10.5061/dryad.3xsj3txw1](https://doi.org/10.5061/dryad.3xsj3txw1) ## DATASET SUMMARY This dataset contains raw and processed experimental data supporting the manuscript “Organ-specific redox imbalance in spinal muscular atrophy mice and partial rescue by SMN antisense oligonucleotides.” File formats: * TIFF files (raw Western blot images) * XLSX spreadsheets (quantification and TBARS data) * TXT README file Software required: * Any software capable of opening TIFF images * Excel or compatible spreadsheet software for XLSX files Reusability: * All data needed to reproduce the figures, quantifications, and statistical analyses are included. Overview This dataset contains all raw and processed data supporting the findings of the manuscript. It includes: * Raw Western blot image files (uncropped TIFFs) * Raw lipid peroxidation (TBARS/MDA) measurements from TECAN plate reader * Processed TBARS values (normalized per mg protein and WT-normalized) * Complete quantification tables for all figures and supplementary figures (ready for reproduction in GraphPad Prism) * Full documentation of standard curve parameters, excluded points, and data-processing steps All files are organized into transparent, reproducible folders. Folder Structure of Dataset_SMA_Multiorgan_Study.zip : ------------------------------------- ### A. Raw_WesternBlot_Images/ Contains the uncropped, unprocessed TIFF images for all Western blots included in the manuscript. Contains additional Western blots used as independent experiments for statistics. Subfolders: Figure_2-7 Supplementary_Figures S1 Supplementary Figures S3 Supplementary Figures S5 Supplementary Figures S6 Supplementary Figures S8 Supplementary Figures S10 Supplementary Figures S11 Supplementary Figures S12 Supplementary Figures S13 Supplementary Figures S14 Each folder contains: The raw TIFF images corresponding to each blot panel ### B. LipidPeroxidation_TBARS_Raw_and_ProcessedData/ Contains the raw and processed lipid peroxidation data (MDA/TBARS assay). Files: #### B1. LipidPeroxidation_TBARS_Brain_SpinalCord_Heart_RawProcessed.xlsx Raw TECAN absorbance values (OD 532 nm) Standard curve wells and technical replicates Standard curve regression parameters Excluded concentrations with justification Processed MDA equivalents (nmol), normalization per mg protein, final WT-normalized values Notes describing processing steps in detail #### B2. LipidPeroxidation_TBARS_Heart_ASOs_RawProcessed.xlsx Includes all individual biological plates Each plate contains: raw values, annotation, standard curve, processed samples For Plate 5, both initial and corrected calculations are shown in separate sheets, together with comments explaining standard-curve linearity and replicate exclusion A final Notes sheet documents all processing steps, formulas, and decisions ### C. QuantificationData_AllFigures.xlsx Contains the final values used to generate all graphs in the manuscript and supplementary material. Sheets include: Figures 2-7 Supplementary Figures S1-S14 Each sheet contains: Final normalized values exactly as used in GraphPad Prism Biological replicate annotations Values provided here match exactly those used in GraphPad Prism. Sufficient detail for full reproducibility **Processing Notes (TBARS Assays)** A complete description is also present inside each .xlsx file under the Notes tab. 3.1 Raw OD values * Exported directly from TECAN SAFIRE II. * Technical duplicates were measured for each well. 3.2 Blank correction * Blank wells (0 nmol MDA) were averaged. * All standard and sample OD values were blank-subtracted: * Adjusted_OD = OD_raw – OD_blank 3.3 Standard curve * Standard concentrations: 0, 2, 6, 10 nmol MDA. * Some concentrations (4 and 8 nmol) were excluded when they deviated from linearity. * Standard curve equation: * MDA_equivalents (nmol) = slope × Adjusted_OD + intercept * Regression parameters recorded in each plate sheet. 3.4 Sample processing For each biological replicate: Raw OD = average of technical replicates Adjusted OD = Raw OD – Average blank OD MDA equivalents (nmol) = slope × Adjusted OD + intercept Protein correction: protein_mg = protein_µg / 1000 MDA_normalized (nmol/mg) = MDA_equivalents / protein_mg Dilution factor correction applied where necessary WT normalization Each plate's WT mean was set to 1 for inter-plate comparability: Prism_normalized_value = MDA_normalized / WT_mean 3.5 Special note for Heart ASO Plate 5 * Both initial and corrected calculations are shown. * The corrected version excludes one non-linear technical replicate from the standard curve. * The exclusion was based solely on deviation from linearity and does not materially affect biological interpretation. ## Western Blot Data Processing The dataset includes only raw TIFF files, without cropping or contrast adjustments. No filtering, resizing, or background subtraction was performed prior to saving the raw images. All quantifications were performed in GraphPad Prism using densitometry from the main manuscript. Western blot TIFF images are named according to the following structure: Figure#_Panel_Target_Tissue_Genotype[_Treatment][_merged].tif Where: Figure#_Panel = Corresponds to the figure number and panel designation as presented in the published manuscript (e.g., Figure3, Figure2A). Target = Protein analyzed (e.g.,GPX4, GRX2, GSTp, GSR, SMN). Tissue = Brain, Spinal cord, Heart, Liver, or Gastrocnemius. Genotype = WT (wild type),HET (heterozygous), SMA. Treatment = ASO (antisense oligonucleotide treatment), included where applicable. \_merged = Composite image used for figure presentation, showing the target protein together with the corresponding loading control (e.g., vinculin or total protein stain). All TIFF files represent uncropped, unprocessed raw Western blot images corresponding directly to the quantifications provided in the processed data files. ## Reproducibility This dataset provides everything necessary to: Reproduce the manuscript figures Refit TBARS standard curves Recalculate normalized MDA values Verify Western blot quantifications Recreate all bar graphs and statistics ## Contact For questions regarding the dataset or analyses, please contact the corresponding author listed in the manuscript.