cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7519277
title: DIFFERENT LEVELS OF BETAINE IN RATION OF SWINE ON STRUCTURE AND ULTRA-STRUCTURE OF INTESTINAL MUCOUS

description: Abstract We evaluated the effects of increasing levels of betaine (0.0%, 0.1%, 0.2%, 0.3%) on small intestine structure. Fifty-two weaned piglets at 23 days of age and average weight of 5.4 kg were used. We studied four betaine levels and five slaughtered times with three days of interval, considering the weaning day as the initial day (zero day). We evaluated villi height and crypt depth. We observed significant differences (P&lt;0.05) among treatments for villi height in duodenum and for crypt depth in ileum. Regarding post-weaning days, significant differences (P&lt;0.05) in villi height and crypt depth occurred in the three intestinal segments analyzed.
DOI: 10.6084/m9.figshare.7519277.v1
title: DIFFERENT LEVELS OF BETAINE IN RATION OF SWINE ON STRUCTURE AND ULTRA-STRUCTURE OF INTESTINAL MUCOUS

description: Abstract We evaluated the effects of increasing levels of betaine (0.0%, 0.1%, 0.2%, 0.3%) on small intestine structure. Fifty-two weaned piglets at 23 days of age and average weight of 5.4 kg were used. We studied four betaine levels and five slaughtered times with three days of interval, considering the weaning day as the initial day (zero day). We evaluated villi height and crypt depth. We observed significant differences (P&lt;0.05) among treatments for villi height in duodenum and for crypt depth in ileum. Regarding post-weaning days, significant differences (P&lt;0.05) in villi height and crypt depth occurred in the three intestinal segments analyzed.
DOI: 10.6084/m9.figshare.7511150
title: MONITORING OF THE INTESTINAL TRACT PARASITE LOAD AND OF THE SANITARY MANGEMENT AT A PACA BREEDING FARM

description: Abstract Paca (Cuniculus paca Linnaeus, 1766) rearing has shown development in all Latin America countries. Fresh feces samples were collected from the soil and analyzed by Willis Mollay and Hoffmann’s techniques, aiming at accompanying the sanitary management, determining the prevalence of gastrointestinal parasites, and their respective load, of pacas raised in captivity, and evaluating parasite control strategies, between the months of November/2012 and June/2013. A McMaster’s camera was used for quantification. The reading was accomplished by optical microscopy with 25x and 40x lenses, which revealed 21,433 eggs per gram of feces (EPG), with an average of 2,679,12 (± 41.0) Strongyloides spp., and 3,325 EPG, with average of 415.6 (± 57.0) Trychuris spp. Besides, 482,101 oocytes per gram of feces (OPG) were found, with an average of 60,263 (± 15.93) Eimeria spp., and 51 larvae of Strongyloides spp., with an average of 6.3 (± 6.86) larvae. Two eggs of Hymenolepisdiminuta were identified in only one stall. An anthelminthic (albendazole 10%) and trimethopim associated with sulfadiazine were used to treat the disease. We concluded the great index of parasitic infestation presented by the animals, in spite of the anthelminthic treatments, can be associated with the rainfall rates observed, the physical conditions of the instalations, as well as the adequate cleaning and sanitization of the facilities.
DOI: 10.6084/m9.figshare.7511150.v1
title: MONITORING OF THE INTESTINAL TRACT PARASITE LOAD AND OF THE SANITARY MANGEMENT AT A PACA BREEDING FARM

description: Abstract Paca (Cuniculus paca Linnaeus, 1766) rearing has shown development in all Latin America countries. Fresh feces samples were collected from the soil and analyzed by Willis Mollay and Hoffmann’s techniques, aiming at accompanying the sanitary management, determining the prevalence of gastrointestinal parasites, and their respective load, of pacas raised in captivity, and evaluating parasite control strategies, between the months of November/2012 and June/2013. A McMaster’s camera was used for quantification. The reading was accomplished by optical microscopy with 25x and 40x lenses, which revealed 21,433 eggs per gram of feces (EPG), with an average of 2,679,12 (± 41.0) Strongyloides spp., and 3,325 EPG, with average of 415.6 (± 57.0) Trychuris spp. Besides, 482,101 oocytes per gram of feces (OPG) were found, with an average of 60,263 (± 15.93) Eimeria spp., and 51 larvae of Strongyloides spp., with an average of 6.3 (± 6.86) larvae. Two eggs of Hymenolepisdiminuta were identified in only one stall. An anthelminthic (albendazole 10%) and trimethopim associated with sulfadiazine were used to treat the disease. We concluded the great index of parasitic infestation presented by the animals, in spite of the anthelminthic treatments, can be associated with the rainfall rates observed, the physical conditions of the instalations, as well as the adequate cleaning and sanitization of the facilities.
DOI: 10.6084/m9.figshare.7507202
title: HYSTOPATHOLOGY AND Leishmania sp. IMMUNOSTAINING IN THIRD EYELID OF Leishmania (Leishmania) chagasi NATURALLY INFECTED DOGS

description: Abstract Canine visceral leishmaniasis (CVL) is a disease with multisystemic injuries, and among the various tissues affected, the third eyelid (TE) is often involved. This ocular adnexa has been studied for the leishmaniasis pathogenesis elucidation and for diagnosis advance. This study aimed to carry out the parasite immunodetection and evaluate histologic lesions in TE of Leishmania (leishmania) chagasi naturally infected dogs. Twenty-six TE samples from infected dogs were submitted to histologic evaluation and to immunohistochemistry for Leishmania sp. The main lesion observed in the third eyelid conjunctiva (TEC) was lymphoplasmacytic inflammation, with Mott cells and parasitized histiocytes. Additionally, loss of epithelial stratification, ulceration, goblet cells thinning or hyperplasia were often found. The same inflammatory pattern was observed in the third eyelid lacrimal gland (TELG), often accompanied by acinar atrophy and secretory ducts dilatation. Immunohistochemistry revealed parasitism in all samples, in different intensities.
DOI: 10.6084/m9.figshare.7507202.v1
title: HYSTOPATHOLOGY AND Leishmania sp. IMMUNOSTAINING IN THIRD EYELID OF Leishmania (Leishmania) chagasi NATURALLY INFECTED DOGS

description: Abstract Canine visceral leishmaniasis (CVL) is a disease with multisystemic injuries, and among the various tissues affected, the third eyelid (TE) is often involved. This ocular adnexa has been studied for the leishmaniasis pathogenesis elucidation and for diagnosis advance. This study aimed to carry out the parasite immunodetection and evaluate histologic lesions in TE of Leishmania (leishmania) chagasi naturally infected dogs. Twenty-six TE samples from infected dogs were submitted to histologic evaluation and to immunohistochemistry for Leishmania sp. The main lesion observed in the third eyelid conjunctiva (TEC) was lymphoplasmacytic inflammation, with Mott cells and parasitized histiocytes. Additionally, loss of epithelial stratification, ulceration, goblet cells thinning or hyperplasia were often found. The same inflammatory pattern was observed in the third eyelid lacrimal gland (TELG), often accompanied by acinar atrophy and secretory ducts dilatation. Immunohistochemistry revealed parasitism in all samples, in different intensities.
DOI: 10.6084/m9.figshare.7518587
title: INTRAOCULAR PRESSURE, MEAN ARTERIAL BLOOD PRESSURE AND PUPILLARY DIAMETER IN RABBITS ( (Oryctolagus cuniculus) ) SUBJECTED TO RETROBULBAR BLOCK WITH DIFFERENT ANESTHETIC PROTOCOLS

description: Abstract The aim of this study was to evaluate four protocols of loco regional anesthesia for ophthalmic procedures that could provide safety and life support, in addition to maintain intraocular pressure stable, with eye centralization and eyelid akinesia. 20 New Zealand rabbits were used to perform local anesthesia by retrobulbar block with four protocols: 2% lidocaine with epinephrine, 2% lidocaine without epinephrine associated with tramadol, 1% ropivacaine and bupivacaine 0.5 %. Each animal received an anesthetic volume of 1 mL. All anesthetic protocols used promoted eyelid akinesia and centralization of the eye during the assessment period. The retrobulbar block with the proposed anesthetic protocols proved to be feasible and safe for the maintenance of intraocular pressure, invasive blood pressure and pupillary diameter and can be used in intraocular surgeries, respecting the time of action of each anesthetic. All protocols showed an excellent blockage action but bupivacaine promoted the highest pupil diameter compared to the other drugs tested.
DOI: 10.6084/m9.figshare.7518587.v1
title: INTRAOCULAR PRESSURE, MEAN ARTERIAL BLOOD PRESSURE AND PUPILLARY DIAMETER IN RABBITS ( (Oryctolagus cuniculus) ) SUBJECTED TO RETROBULBAR BLOCK WITH DIFFERENT ANESTHETIC PROTOCOLS

description: Abstract The aim of this study was to evaluate four protocols of loco regional anesthesia for ophthalmic procedures that could provide safety and life support, in addition to maintain intraocular pressure stable, with eye centralization and eyelid akinesia. 20 New Zealand rabbits were used to perform local anesthesia by retrobulbar block with four protocols: 2% lidocaine with epinephrine, 2% lidocaine without epinephrine associated with tramadol, 1% ropivacaine and bupivacaine 0.5 %. Each animal received an anesthetic volume of 1 mL. All anesthetic protocols used promoted eyelid akinesia and centralization of the eye during the assessment period. The retrobulbar block with the proposed anesthetic protocols proved to be feasible and safe for the maintenance of intraocular pressure, invasive blood pressure and pupillary diameter and can be used in intraocular surgeries, respecting the time of action of each anesthetic. All protocols showed an excellent blockage action but bupivacaine promoted the highest pupil diameter compared to the other drugs tested.
DOI: 10.6084/m9.figshare.7514105
title: SUPPLEMENTATION WITH PROTEIN SOURCES IN THE FINISHING STAGE OF BEEF HEIFERS: A BIOECONOMICAL STUDY

description: Abstract The purpose of this study was to evaluate biological and economically the use of three protein sources (soybean meal (SM), cottonseed (CS) or turnip cake (TC)), in the supplement formulation (1.0 % of body weight (BW)) in the finishing stage of heifers grazing Marandu grass pastures during the dry season. This experiment was conducted in the city of Potirendaba, São Paulo State, and the chemical analyses were performed at the Londrina State University, Paraná State. The experimental area had six hectares, divided into three paddocks, each one with six crossbred heifers (Simmental × Nellore), initial BW of 210 ± 8.34 kg, and age of 15 ± 3 months. Economic analysis was performed using the method of partial budgeting. The experimental design was completely randomized, with six replicates per treatment (n = 18). Analysis of variance was conducted, and means were compared by Tukey test (P &lt;0.05). Animals supplemented with TC exhibited lower weight gain (SM = 0.81, CS = 0.76 and TC = 0.40 kg / animal / day; P &lt;0.05). Negative changes were observed in net income for the CS supplements (US $ -22.15 / animal) and TC (US $ -1.96 / animal) relative to the SM supplement, which showed better biologic and economic results.
DOI: 10.6084/m9.figshare.7514105.v1
title: SUPPLEMENTATION WITH PROTEIN SOURCES IN THE FINISHING STAGE OF BEEF HEIFERS: A BIOECONOMICAL STUDY

description: Abstract The purpose of this study was to evaluate biological and economically the use of three protein sources (soybean meal (SM), cottonseed (CS) or turnip cake (TC)), in the supplement formulation (1.0 % of body weight (BW)) in the finishing stage of heifers grazing Marandu grass pastures during the dry season. This experiment was conducted in the city of Potirendaba, São Paulo State, and the chemical analyses were performed at the Londrina State University, Paraná State. The experimental area had six hectares, divided into three paddocks, each one with six crossbred heifers (Simmental × Nellore), initial BW of 210 ± 8.34 kg, and age of 15 ± 3 months. Economic analysis was performed using the method of partial budgeting. The experimental design was completely randomized, with six replicates per treatment (n = 18). Analysis of variance was conducted, and means were compared by Tukey test (P &lt;0.05). Animals supplemented with TC exhibited lower weight gain (SM = 0.81, CS = 0.76 and TC = 0.40 kg / animal / day; P &lt;0.05). Negative changes were observed in net income for the CS supplements (US $ -22.15 / animal) and TC (US $ -1.96 / animal) relative to the SM supplement, which showed better biologic and economic results.
DOI: 10.6084/m9.figshare.7506626
title: PHYSICAL AND MICROBIOLOGICAL QUALITY OF OPAQUE, SANITIZED, AND CHILLED QUAIL EGGS EXPERIMENTALLY CONTAMINATED WITH Salmonella enteric SER. TYPHIMURIUM

description: Abstract The objective of this study was to verify the physical, chemical and microbiological quality of Japanese quail eggs artificially contaminated withSalmonella enterica ser. Typhimurium. The eggs were sanitized and stored at different temperatures (between 5 and 25 ºC) for 27 days. We used 768 eggs with opaque shells, typical pigments of the species, and average weight of 11 g. The experimental design was completely randomized in a 2x2x2 factorial arrangement (contamination x sanitation x cooling) with six replications and one egg per experimental unit. The eggs were contaminated by handling with 1.5 x 105 colony forming unit (CFU) ofSalmonella. Typhimurium / mL and sanitized according to the treatments with a 5 ppm Cl solution. The data were subjected to analysis of variance and t test. Bacterial contamination has damaged the egg weight, Haugh unit, yolk index and albumen, and pH of yolk and albumen, from 18 days of storage. The egg storage time and storage temperature affected the internal quality of quail eggs in all variables. The worst internal quality was observed in eggs stored at 25 ºC. The sanitation and cooling reduced the growth of Salmonella in contaminated eggs. Eggs in opaque shell, when not refrigerated, should be consumed within 18 days after laying.
DOI: 10.6084/m9.figshare.7506626.v1
title: PHYSICAL AND MICROBIOLOGICAL QUALITY OF OPAQUE, SANITIZED, AND CHILLED QUAIL EGGS EXPERIMENTALLY CONTAMINATED WITH Salmonella enteric SER. TYPHIMURIUM

description: Abstract The objective of this study was to verify the physical, chemical and microbiological quality of Japanese quail eggs artificially contaminated withSalmonella enterica ser. Typhimurium. The eggs were sanitized and stored at different temperatures (between 5 and 25 ºC) for 27 days. We used 768 eggs with opaque shells, typical pigments of the species, and average weight of 11 g. The experimental design was completely randomized in a 2x2x2 factorial arrangement (contamination x sanitation x cooling) with six replications and one egg per experimental unit. The eggs were contaminated by handling with 1.5 x 105 colony forming unit (CFU) ofSalmonella. Typhimurium / mL and sanitized according to the treatments with a 5 ppm Cl solution. The data were subjected to analysis of variance and t test. Bacterial contamination has damaged the egg weight, Haugh unit, yolk index and albumen, and pH of yolk and albumen, from 18 days of storage. The egg storage time and storage temperature affected the internal quality of quail eggs in all variables. The worst internal quality was observed in eggs stored at 25 ºC. The sanitation and cooling reduced the growth of Salmonella in contaminated eggs. Eggs in opaque shell, when not refrigerated, should be consumed within 18 days after laying.
DOI: 10.6084/m9.figshare.7512758
title: CRUDE GLYCERIN IN DIETS FOR PIGLETS IN NURSING PHASE

description: Abstract This study evaluated the inclusion of crude glycerin for piglets in the starter phase. In the performance assay, we used 80 barrows, with initial weight of 6.64 ± 0.8 kg. The experimental design was of randomized blocks and the treatments consisted in 0%, 2%, 4% and 6% of crude glycerin. There was a quadratic effect on final weight, average daily gain and feed intake, the maximum inclusion of crude glycerin was of 2.77%, for higher daily intake. For the ADG, the level of 2.76% of crude glycerin allowed the best gain and final weight, despite any effect on food conversion. There was a significant effect on the incidence of diarrhea (p=0.014) when 2% of crude glycerin was included in the diet. There was a linear decreasing effect on the percentage of GIT (p=0,006), small intestine (p=0,038), kidneys (p=0,089) and liver (p=0,037) of piglets that did not have glycerin in the diet. We observed a quadratic effect on the relative weight of the spleen and pancreas of piglets, the level of 2.73% of crude glycerin resulted in lower pancreas weight, and 3.04% of glycerin increased spleen weight. The level of 2.76% of crude glycerin promotes the highest weight gain for piglets in the nursery phase.
DOI: 10.6084/m9.figshare.7512758.v1
title: CRUDE GLYCERIN IN DIETS FOR PIGLETS IN NURSING PHASE

description: Abstract This study evaluated the inclusion of crude glycerin for piglets in the starter phase. In the performance assay, we used 80 barrows, with initial weight of 6.64 ± 0.8 kg. The experimental design was of randomized blocks and the treatments consisted in 0%, 2%, 4% and 6% of crude glycerin. There was a quadratic effect on final weight, average daily gain and feed intake, the maximum inclusion of crude glycerin was of 2.77%, for higher daily intake. For the ADG, the level of 2.76% of crude glycerin allowed the best gain and final weight, despite any effect on food conversion. There was a significant effect on the incidence of diarrhea (p=0.014) when 2% of crude glycerin was included in the diet. There was a linear decreasing effect on the percentage of GIT (p=0,006), small intestine (p=0,038), kidneys (p=0,089) and liver (p=0,037) of piglets that did not have glycerin in the diet. We observed a quadratic effect on the relative weight of the spleen and pancreas of piglets, the level of 2.73% of crude glycerin resulted in lower pancreas weight, and 3.04% of glycerin increased spleen weight. The level of 2.76% of crude glycerin promotes the highest weight gain for piglets in the nursery phase.
DOI: 10.6084/m9.figshare.7514702
title: SEASONAL INFLUENCE ON SEMINAL PATTERN AND FREEZABILITY OF PANTANEIRO BULL SEMEN

description: Abstract The purpose of this paper was to investigate the effect of the season of the year on testicular morphology, fresh and frozen/thawed semen quality from Pantaneiro bulls bred in Brasilia, DF. Six bulls were submitted, once a month for one year, to evaluation of testicular measurements, semen collection using an artificial vagina and cryopreserved with medium containing Tris-based, egg yolk and glycerol. The testicular length and volume were greater (P &lt; 0.05) in June (11.1±0.8 cm; 652.7±138.0 cm3), July (11.0±0.9 cm; 616.4±156.2 cm3), and September (11.8±0.8 cm; 692.4±141.3 cm3) than October (9.4±1.2 cm; 498.5±154.3 cm3) and November (10.3±1.1 cm; 560.0±111.8 cm3). The sperm concentration was greater (P&lt;0.05) in August (1265.0±436.8 x106sperm/mL) than October, December and January (591.6±192.0, 627.5±314.4; 502.5±172.9 x106/mL). There was no effect of season on the quality of fresh and frozen semen (motility, vigor, total defects and acrosome integrity) (P &gt; 0.05). In conclusion, Pantaneiro bulls, bred in the Midwest region of Brazil, can be used in natural mating programs in all seasons. Aiming to improve the Brazilian Animal Germplasm Bank with a higher number of samples collected, we suggest that the freezing of the semen is done between June and August, although there will be no losses of semen quality at any time.
DOI: 10.6084/m9.figshare.7507448.v1
title: MAIN CAUSES OF CONDEMNATION IN POULTRY SLAUGHTERHOUSES REGISTERED IN BRAZILIAN FEDERAL INSPECTION SERVICE FROM 2006 TO 2011

description: Abstract The poultry industry in Brazil as well as the domestic consumption and exportation of poultry have experienced significant growth. The Federal Inspection Service is responsible for the sanitary inspection of poultry slaughter, and it routinely records injuries or illnesses that have been identified in the Management Information System of the Federal Inspection Service (SIGSIF). The aim of this study was to evaluate the main causes of condemnation in poultry slaughterhouses registered in the Federal Inspection Service from data entered into the SIGSIF system from 2006 to 2011. The total rate of condemnations observed during this period was 5.99%, and the major causes were contamination (1.80%), contusion /traumatic injuries (1.57%), dermatosis (0.74%) and cellulitis (0, 50%). Regional variations were observed in the occurrence of condemnations, and only in case of dermatosis a seasonal behavior was observed. The SIGSIF is an important source of information concerning the causes of condemnations in poultry slaughter and can be a useful tool for assessing the causes and proposing measures to prevent or mitigate risks.
DOI: 10.6084/m9.figshare.7506764.v1
title: USE OF HYDROALCOHOLIC EXTRACT OF Schinus terebinthifolius Raddi IN PRE- AND POST-MILKING ANTISEPSIS OF THE TEAT IN DAIRY COWS

description: Abstract Medicinal plants have been used for centuries as an alternative treatment for health problems. Schinus terebinthifolius Raddi is a median tree that belongs to the Anacardiaceae family. The antibacterial effect of leaves extract of this plant has already been demonstrated. The objective of this study was to test a substance derived from this plant to be used in pre- and post-milking teat antisepsis. The hydroalcoholic extract of Brazilian pepper tree was used in opposite quarters for twelve consecutive weeks, and commercial iodine was used as control. None of the indices analyzed - black background mug, CMT, intramammary infections, skin health, and teat health - showed a statistical difference between the treatments, suggesting that the plant extract can be used in pre- and post-milking teat antisepsis, as a substitute for conventional products for herds in an agroecological production system.
DOI: 10.6084/m9.figshare.7507448
title: MAIN CAUSES OF CONDEMNATION IN POULTRY SLAUGHTERHOUSES REGISTERED IN BRAZILIAN FEDERAL INSPECTION SERVICE FROM 2006 TO 2011

description: Abstract The poultry industry in Brazil as well as the domestic consumption and exportation of poultry have experienced significant growth. The Federal Inspection Service is responsible for the sanitary inspection of poultry slaughter, and it routinely records injuries or illnesses that have been identified in the Management Information System of the Federal Inspection Service (SIGSIF). The aim of this study was to evaluate the main causes of condemnation in poultry slaughterhouses registered in the Federal Inspection Service from data entered into the SIGSIF system from 2006 to 2011. The total rate of condemnations observed during this period was 5.99%, and the major causes were contamination (1.80%), contusion /traumatic injuries (1.57%), dermatosis (0.74%) and cellulitis (0, 50%). Regional variations were observed in the occurrence of condemnations, and only in case of dermatosis a seasonal behavior was observed. The SIGSIF is an important source of information concerning the causes of condemnations in poultry slaughter and can be a useful tool for assessing the causes and proposing measures to prevent or mitigate risks.