DOI: 10.5061/dryad.37pvmcvh4
title: Data from: Ultraconserved elements reconstruct the evolution of the Chagas disease-vectoring kissing bugs (Reduviidae: Triatominae)

description: With about 150 species, Triatominae, the kissing bugs, are the largest
 radiation of hematophagous species within the Hemiptera. Kissing bugs are
 the sole vectors of the causative agent of Chagas disease, a tropical
 neglected disease that affects millions, mostly in Central and South
 America. Surprisingly, given the medical importance of this group, the
 evolutionary origin of Triatominae from predatory assassin bug ancestors
 is still under debate and phylogenetic relationships among and within the
 five tribes of Triatominae are poorly understood. We here use a
 combination of ultraconserved element (UCE) and ribosomal data generated
 from ethanol-preserved and pinned museum specimens to generate the first
 data-rich and taxonomically densely sampled, well-supported phylogenetic
 hypothesis for this group of important human disease vectors. This study
 is the first to include multiple species and/or genera of four of the five
 currently recognized tribes and is significant in being the first
 phylogeny to include substantial diversity of Old World Triatominae. We
 examine tribal and generic concepts as well as species groups, subgroups,
 complexes that are widely referred to in the epidemiological literature on
 kissing bugs concluding that based on this dataset: 1) Triatominae are
 monophyletic and Opisthacidius Berg is their predatory sister taxon; 2)
 (Cavernicolini + Microtriatoma Prosen and Martinez [Bolboderini] +
 Rhodniini) is the sister lineage of (Belminus Stål [Bolboderini] +
 Triatomini); 3) the three large genera (Rhodnius Stål, Triatoma Laporte,
 and Panstrongylus Berg) are paraphyletic, as previously suggested; 4)
 Triatomini fall into nine well-supported clades, only two of which are
 identical in composition to previously recognized groups; 5) the Old World
 clade is nested within a clade also comprising the rubida and protracta
 clades, and is sister to the protracta clade; 6) body size and shape, head
 proportion, coloration, and several additional morphological features
 evolved plastically in Triatominae, suggesting that most of them are
 unsuitable for the diagnosis of higher taxonomic groups.
description: Briefly, DNA were extracted from museum and recently collected
 specimens of Triatominae. Genomic libraries were generated and target
 enrichment with Hemiptera v1 UCE baits was performed. Data were run
 through the PHYLUCE pipeline to extract UCE loci. We also extracted
 additional UCE loci from transcriptome data and ribosomal operon data
 available on Genbank. 
description: <strong>phyluce_workflow.txt</strong>
 Provides the order of steps and name of scripts submitted for the
 analysis.
 <strong>example_scripts</strong>
 Zip folder of scripts submitted to an HPC cluster for
 analysis.
 <strong>conf_files</strong> Zip
 folder of configuration files used in PHYLUCE for illuminprocessor,
 assembly, and the taxon-set. Also included the hemiptera-v1 bait set used
 for UCE loci identification and extraction.
 <strong>UCE_Trees</strong> Zip
 folder of UCE loci alignments for phylogentics tree anlyses and partition
 files where applicable for Astral, MrBayes, RAxML,
 PartitionFinder2.
 <strong>UCE_operon_Trees</strong>
 Zip folder of UCE with ribosomal operons for phylogentics tree
 anlyses and partition files where applicable fo MrBayes, RAxML.