cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14275500.v1
title: Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species

description: Abstract Candida is a human fungal pathogen that causes a wide range of diseases. Candida albicans is the main etiologic agent in these diseases; however, infections can be caused by non-albicans Candida species. Virulence factors such as biofilm production, which protect the fungus from host immunity and anti-fungal drugs, are important for the infection. Therefore, available antifungal drugs for candidiasis treatment are limited and the investigation of new and effective drugs is needed. Verapamil is a calcium channel blocker with an inhibitory effect on hyphae development, adhesion, and colonization of C. albicans. In this study, we investigated the effect of verapamil on cell viability and its antifungal and anti-biofilm activity in non-albicans Candida species. Verapamil was not toxic to keratinocyte cells; moreover, C. krusei, C. parapsilosis, and C. glabrata were susceptible to verapamil with a minimal inhibitory concentration (MIC) of 1250 μM; in addition, this drug displayed fungistatic effect at the evaluated concentrations. After treatment with verapamil, reduced viability, biomass, and mitochondrial activity were observed in biofilms of the non-albicans Candida species C. krusei, C. glabrata, and C. parapsilosis. These findings highlight the importance of the study of verapamil as an alternative treatment for infections caused by non-albicans Candida species.
DOI: 10.6084/m9.figshare.14275503
title: Distribution and origin of sedimentary organic matter in an eutrophic estuary: Pina Sound – NE Brazil

description: Abstract Eutrophic estuaries receive organic matter (OM) inputs from multiple sources. This study evaluated the distribution and origin of sedimentary OM in an eutrophic estuary (Pina Sound, NE Brazil). Thirteen samples were collected in the sublittoral in addition to major local sources. Biochemical (chlorophyll - Chl), elemental [(C/N)a and C/S ratios] and isotopic (δ 15N and δ 13C) analyses were carried out for characterizing OM and redox conditions. The SIAR mixing model was used to quantify contribution from main sources. At Pina Sound, distribution of OM is associated with mud, reflecting the hydrodynamics control on deposition of suspended particles. Microphytobenthic production is limited ([Chl a] &lt; 1000 µg/g organic carbon) in the sublittoral where the Chl degradation products prevail (mean [Pheopigments] = 2643 ± 958 µg/g organic carbon). Anoxic conditions (C/S ratio ≈ 2) are typically observed in sediments of deeper portions of Pina Sound. Such sediments receive high organic loads and are subject to poor water renewal. According to SIAR mixing model, sedimentary OM of Pina Sound is composed of, on average: 50% phytoplankton, 24% sewage and 26% C3 plants. This reflects fertilization of Pina Sound with high loads of untreated sewage. Pina Sound has a great potential to retain sewage-derived OM.
DOI: 10.6084/m9.figshare.14275503.v1
title: Distribution and origin of sedimentary organic matter in an eutrophic estuary: Pina Sound – NE Brazil

description: Abstract Eutrophic estuaries receive organic matter (OM) inputs from multiple sources. This study evaluated the distribution and origin of sedimentary OM in an eutrophic estuary (Pina Sound, NE Brazil). Thirteen samples were collected in the sublittoral in addition to major local sources. Biochemical (chlorophyll - Chl), elemental [(C/N)a and C/S ratios] and isotopic (δ 15N and δ 13C) analyses were carried out for characterizing OM and redox conditions. The SIAR mixing model was used to quantify contribution from main sources. At Pina Sound, distribution of OM is associated with mud, reflecting the hydrodynamics control on deposition of suspended particles. Microphytobenthic production is limited ([Chl a] &lt; 1000 µg/g organic carbon) in the sublittoral where the Chl degradation products prevail (mean [Pheopigments] = 2643 ± 958 µg/g organic carbon). Anoxic conditions (C/S ratio ≈ 2) are typically observed in sediments of deeper portions of Pina Sound. Such sediments receive high organic loads and are subject to poor water renewal. According to SIAR mixing model, sedimentary OM of Pina Sound is composed of, on average: 50% phytoplankton, 24% sewage and 26% C3 plants. This reflects fertilization of Pina Sound with high loads of untreated sewage. Pina Sound has a great potential to retain sewage-derived OM.
DOI: 10.6084/m9.figshare.14275506
title: In-vitro Antibacterial and Antibiofilm Activity of Cinnamomum verum Leaf Oil against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae

description: Abstract Phytomedicines are becoming more popular in treatment of infectious diseases worldwide. Cinnamomum verum essential oil (EO) has been used as a therapeutic alternative for various diseases. This study aimed to evaluate the antibacterial and antibiofilm activity of the C. verum leaf EO against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. Effect of EO vapor on planktonic cells was determined using microatmosphere technique. CLSI M7-A10 method was employed in Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) experiments. Effect of EO on established biofilms was quantified and visualized using XTT and Scanning Electron Microscope (SEM). In-vitro toxicity was evaluated using Human Keratinocytes (HaCaT). Chemical analysis of EO was done using Gas Chromatography- Mass Spectrometry (GC-MS). All tested strains were sensitive to cinnamon oil vapor. EO exhibited 0.5 and 1.0 mg/mL MIC and MBC against all test strains. Minimum Biofilm Inhibitory and Biofilm Eradication Concentrations (MBIC50 and MBEC) were 1.0 and 4.0 mg/mL. SEM indicated cellular shrinkages, cell wall damages, and decreased biofilm densities. Cinnamon oil didn’t show any toxicity on HaCaT cell at any concentration tested. Eugenol was the most abundant compound in C. verum oil. C. verum EO shows an antibacterial and antibiofilm activity with minimal toxicity on host.
DOI: 10.6084/m9.figshare.14275508
title: Landslide susceptibility mapping using the statistical method of Information Value: A study case in Ribeirão dos Macacos basin, Minas Gerais, Brazil

description: Abstract This research study was developed in the Ribeirão dos Macacos basin at the district of Nova Lima, Minas Gerais state, Brazil. The information value statistical method was applied in the construction of the landslide susceptibility map at the 1:25,000 scale. Different partitions of the inventory were tested, as well as different landslide predisposing factors. In the construction of the landslide inventory, the south, southeast and south-west slopes generally present a higher quality in aerial / orbital images due to the position of the sun (lighting direction), which emphasizes the surface structures and it may omit old landslides on slopes facing north, northeast, and northwest. This condition can generate misleading models when using the slope aspect. Another verification was that the models with better Area Under the Curve index will not always restrict the high susceptibility class in smaller areas. This incongruence occurs due to the different curve conformations, since a smaller index curve can present more restrictive results than a larger index curve. The results showed that the model has a high capacity of adjustment to the input data and high landslide predictive capacity.
DOI: 10.6084/m9.figshare.14275506.v1
title: In-vitro Antibacterial and Antibiofilm Activity of Cinnamomum verum Leaf Oil against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae

description: Abstract Phytomedicines are becoming more popular in treatment of infectious diseases worldwide. Cinnamomum verum essential oil (EO) has been used as a therapeutic alternative for various diseases. This study aimed to evaluate the antibacterial and antibiofilm activity of the C. verum leaf EO against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. Effect of EO vapor on planktonic cells was determined using microatmosphere technique. CLSI M7-A10 method was employed in Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) experiments. Effect of EO on established biofilms was quantified and visualized using XTT and Scanning Electron Microscope (SEM). In-vitro toxicity was evaluated using Human Keratinocytes (HaCaT). Chemical analysis of EO was done using Gas Chromatography- Mass Spectrometry (GC-MS). All tested strains were sensitive to cinnamon oil vapor. EO exhibited 0.5 and 1.0 mg/mL MIC and MBC against all test strains. Minimum Biofilm Inhibitory and Biofilm Eradication Concentrations (MBIC50 and MBEC) were 1.0 and 4.0 mg/mL. SEM indicated cellular shrinkages, cell wall damages, and decreased biofilm densities. Cinnamon oil didn’t show any toxicity on HaCaT cell at any concentration tested. Eugenol was the most abundant compound in C. verum oil. C. verum EO shows an antibacterial and antibiofilm activity with minimal toxicity on host.
DOI: 10.6084/m9.figshare.14275508.v1
title: Landslide susceptibility mapping using the statistical method of Information Value: A study case in Ribeirão dos Macacos basin, Minas Gerais, Brazil

description: Abstract This research study was developed in the Ribeirão dos Macacos basin at the district of Nova Lima, Minas Gerais state, Brazil. The information value statistical method was applied in the construction of the landslide susceptibility map at the 1:25,000 scale. Different partitions of the inventory were tested, as well as different landslide predisposing factors. In the construction of the landslide inventory, the south, southeast and south-west slopes generally present a higher quality in aerial / orbital images due to the position of the sun (lighting direction), which emphasizes the surface structures and it may omit old landslides on slopes facing north, northeast, and northwest. This condition can generate misleading models when using the slope aspect. Another verification was that the models with better Area Under the Curve index will not always restrict the high susceptibility class in smaller areas. This incongruence occurs due to the different curve conformations, since a smaller index curve can present more restrictive results than a larger index curve. The results showed that the model has a high capacity of adjustment to the input data and high landslide predictive capacity.
DOI: 10.6084/m9.figshare.14275511
title: Comparative Analysis of Water Stress Management’s Tools Between Alicante - Spain and Caraguatatuba - Brazil

description: Abstract The Alicante’s province, located mostly in Júcar´s Water Basin in Spain, deals with water stress and land development since 1980s. The provisions about climate change in the area suggest that water stress will increase, so it is necessary to integrated it with spatial planning. This issues is also happens in Caraguatatuba, northern coast of São Paulo State in Brazil. There are planning tools developed for assessment the water stress, as Flooding´s Risk Management Plan and Alert and Drought Period Special Plan in Júcar´s Water Basin and Water´s Safety Plan for Caraguatatuba. The main of this paper is to analyze the spanish tools to identify a commum structure for replicated it. Other main is, by the spanish practice, to identify opportunities for improvement the water stress management in Caraguatatuba. As results there are procedures in water stress planning, which deals with land use directives.
DOI: 10.6084/m9.figshare.14275514.v1
title: Extreme Wave Analysis Based on 31 Years Data from WW3 Model: Study off Southern Brazilian Coast

description: Abstract A long-term wave hindcast (1979-2009) based on the NCEP/NCAR Reanalysis and WAVEWATCH III has been assessed using 29 months of wave measurements over Rio Grande shelf, South Brazil to evaluate the skills and biases in the wave hindcast for this region. Extreme events were selected by Peaks Over Thresholds (POT) and fitted by a Generalized Pareto Distribution (GPD) to estimate the extreme significant wave height from the 31 years wave simulation. The significant wave heights from the hinhindcast and measurement show generally good aagreement although the wave heights tended to be underestimated in the hindcast. This underestimation was more pronounced in extreme wave events. The estimated extreme waves, based on a hindcast, with 50 and 100 years return period in the offshore deep water over the Rio Grande shelf, feature large waves heights with 10.54 and 11.18 meters, respectively. The information presented here can be useful for those involved in coastal management and disaster response and also for the navigation and offshore operations.
DOI: 10.6084/m9.figshare.14268451
title: Comparison of spatial interpolation methods for annual and seasonal rainfall in two hotspots of biodiversity in South America

description: Abstract The Colombian Biogeographic Choco (CBC) and the La Plata Basin (LPB) are regions with high biodiversity. However, these areas are characterized by scarce climatological information, complex orography, and rain-gauge network unevenly distributed. Interpolated data from the ground station might overcome these aspects. For this reason, is necessary to identify the best technique for the spatial interpolation of rainfall. Hence, the spatial interpolation techniques were applied to annual and seasonal rainfall in the CBC and LPB. Geostatistical results and deterministic approaches were compared by cross-validation. Cokriging with spherical (gaussian) model is the best interpolator in the CBC (LPB), as indicated by the lowest root mean square error (RMSE) and a standardized RMSE close to one. The CBC shows three rainfall cores: the northern, 9,000 mm/year; the central-southern, 10,000 mm/year; and the southern, 7,000 mm/year. The LPB shows a west-east rainfall gradient, with a minimum to the west (450 mm/year) and a maximum in the mid-west (2,000 mm/year). To the north of the LPB, rainfall reaches 1,500 mm/year, while in the south it reaches only 900 mm/year. The results in our study may be useful for scientists and decision-makers for use in environmental and hydrological models for the CBC and the LPB.
DOI: 10.6084/m9.figshare.14268451.v1
title: Comparison of spatial interpolation methods for annual and seasonal rainfall in two hotspots of biodiversity in South America

description: Abstract The Colombian Biogeographic Choco (CBC) and the La Plata Basin (LPB) are regions with high biodiversity. However, these areas are characterized by scarce climatological information, complex orography, and rain-gauge network unevenly distributed. Interpolated data from the ground station might overcome these aspects. For this reason, is necessary to identify the best technique for the spatial interpolation of rainfall. Hence, the spatial interpolation techniques were applied to annual and seasonal rainfall in the CBC and LPB. Geostatistical results and deterministic approaches were compared by cross-validation. Cokriging with spherical (gaussian) model is the best interpolator in the CBC (LPB), as indicated by the lowest root mean square error (RMSE) and a standardized RMSE close to one. The CBC shows three rainfall cores: the northern, 9,000 mm/year; the central-southern, 10,000 mm/year; and the southern, 7,000 mm/year. The LPB shows a west-east rainfall gradient, with a minimum to the west (450 mm/year) and a maximum in the mid-west (2,000 mm/year). To the north of the LPB, rainfall reaches 1,500 mm/year, while in the south it reaches only 900 mm/year. The results in our study may be useful for scientists and decision-makers for use in environmental and hydrological models for the CBC and the LPB.
DOI: 10.6084/m9.figshare.14275511.v1
title: Comparative Analysis of Water Stress Management’s Tools Between Alicante - Spain and Caraguatatuba - Brazil

description: Abstract The Alicante’s province, located mostly in Júcar´s Water Basin in Spain, deals with water stress and land development since 1980s. The provisions about climate change in the area suggest that water stress will increase, so it is necessary to integrated it with spatial planning. This issues is also happens in Caraguatatuba, northern coast of São Paulo State in Brazil. There are planning tools developed for assessment the water stress, as Flooding´s Risk Management Plan and Alert and Drought Period Special Plan in Júcar´s Water Basin and Water´s Safety Plan for Caraguatatuba. The main of this paper is to analyze the spanish tools to identify a commum structure for replicated it. Other main is, by the spanish practice, to identify opportunities for improvement the water stress management in Caraguatatuba. As results there are procedures in water stress planning, which deals with land use directives.
DOI: 10.6084/m9.figshare.14275514
title: Extreme Wave Analysis Based on 31 Years Data from WW3 Model: Study off Southern Brazilian Coast

description: Abstract A long-term wave hindcast (1979-2009) based on the NCEP/NCAR Reanalysis and WAVEWATCH III has been assessed using 29 months of wave measurements over Rio Grande shelf, South Brazil to evaluate the skills and biases in the wave hindcast for this region. Extreme events were selected by Peaks Over Thresholds (POT) and fitted by a Generalized Pareto Distribution (GPD) to estimate the extreme significant wave height from the 31 years wave simulation. The significant wave heights from the hinhindcast and measurement show generally good aagreement although the wave heights tended to be underestimated in the hindcast. This underestimation was more pronounced in extreme wave events. The estimated extreme waves, based on a hindcast, with 50 and 100 years return period in the offshore deep water over the Rio Grande shelf, feature large waves heights with 10.54 and 11.18 meters, respectively. The information presented here can be useful for those involved in coastal management and disaster response and also for the navigation and offshore operations.
DOI: 10.6084/m9.figshare.14275517
title: Examining of rock drilling properties in underground metal mine excavation

description: Abstract In underground metal mines, drilling and blasting is widely used production method. Drilling holes is especially a time consuming operation. The aim of this study is to examine and analyze drilling operation and its duration in underground ore and dead rock. Drillability experiments are carried out on samples taken from the field. In core sampling two different types of core samples are taken whether it is parallel or perpendicular to ore bedding and dead rock. Results of drillability experiments are compared between the two types of core samples. DRI values are found to be 51 and 47 for the parallel and perpendicular core samples to ore bedding, respectively. DRI values for the parallel and perpendicular core samples to dead rock bedding are found to be 57 and 55 for the, respectively. In addition, graphs of drilling speed and drilling depth belonging to ore and dead rock are analyzed. It is examined that drilling speed increases steadily to maximum point in the first 5sec. and stays stable after that point. Therefore, it is concluded that production galleries and holes which are formed parallel to bedding would increase production efficiency.
DOI: 10.6084/m9.figshare.14275517.v1
title: Examining of rock drilling properties in underground metal mine excavation

description: Abstract In underground metal mines, drilling and blasting is widely used production method. Drilling holes is especially a time consuming operation. The aim of this study is to examine and analyze drilling operation and its duration in underground ore and dead rock. Drillability experiments are carried out on samples taken from the field. In core sampling two different types of core samples are taken whether it is parallel or perpendicular to ore bedding and dead rock. Results of drillability experiments are compared between the two types of core samples. DRI values are found to be 51 and 47 for the parallel and perpendicular core samples to ore bedding, respectively. DRI values for the parallel and perpendicular core samples to dead rock bedding are found to be 57 and 55 for the, respectively. In addition, graphs of drilling speed and drilling depth belonging to ore and dead rock are analyzed. It is examined that drilling speed increases steadily to maximum point in the first 5sec. and stays stable after that point. Therefore, it is concluded that production galleries and holes which are formed parallel to bedding would increase production efficiency.
DOI: 10.6084/m9.figshare.14275521
title: Impacts of agrarian reform on land use in the biomes of the Midwest region of Brazil between 2004 and 2014

description: Abstract Brazil’s Midwest is composed of four biomes: the Cerrado, predominant in the region; the Pantanal, the largest wetland in the world; the Amazon, which occupies part of Mato Grosso; and the Atlantic Forest. The objective of this study was to identify the evolution of occupation and use of land in the rural settlements of the Brazilian Midwest depending on the biome of location. A total of 54 settlements distributed in the four biomes of the region were analyzed using direct observation and Landsat images from the years 2004 and 2014. Using the software QGIS 2.8 Wien, the vegetation indices NDVI and NDWI were used to classify agricultural, pasture and forest areas by biome. Native vegetation is declining in most of the analyzed settlements and pastures, for milk production, occupied the largest area. Between 2004 and 2014, pasture areas expanded to the detriment of forests. Although they have the highest percentage of environmental preservation areas, the settlements we analyzed in the Amazon biome do not comply with legislation. Part of the forest in these settlements was transformed into areas of bushy cerrado. However, there was an increase in forests in the settlements of the Atlantic Forest biome.
DOI: 10.6084/m9.figshare.14275521.v1
title: Impacts of agrarian reform on land use in the biomes of the Midwest region of Brazil between 2004 and 2014

description: Abstract Brazil’s Midwest is composed of four biomes: the Cerrado, predominant in the region; the Pantanal, the largest wetland in the world; the Amazon, which occupies part of Mato Grosso; and the Atlantic Forest. The objective of this study was to identify the evolution of occupation and use of land in the rural settlements of the Brazilian Midwest depending on the biome of location. A total of 54 settlements distributed in the four biomes of the region were analyzed using direct observation and Landsat images from the years 2004 and 2014. Using the software QGIS 2.8 Wien, the vegetation indices NDVI and NDWI were used to classify agricultural, pasture and forest areas by biome. Native vegetation is declining in most of the analyzed settlements and pastures, for milk production, occupied the largest area. Between 2004 and 2014, pasture areas expanded to the detriment of forests. Although they have the highest percentage of environmental preservation areas, the settlements we analyzed in the Amazon biome do not comply with legislation. Part of the forest in these settlements was transformed into areas of bushy cerrado. However, there was an increase in forests in the settlements of the Atlantic Forest biome.