DOI: 10.5281/zenodo.11658230
title: Mechanochemical induced swelling-activation of a gastric-deployable 4D printed polypill inspired by natural hygromorphic actuators - Underlying data

description: Underloying microfocus CT data of Mechanochemical induced swelling-activation of a gastric-deployable 4D printed polypill inspired by natural hygromorphic actuators by Konstantina Chachlioutaki, Nikolaos Papas, Zisis Chatzis, Orestis L. Katsamenis, Stephanie K. Robinson, Konstantinos Tsongas, Nikolaos Bouropoulos, Dimitrios G. Fatouros, Dimitrios Tzetzis, Christina Karavasili



K. Chachlioutaki, Z. Chatzis, D.G. Fatouros, C. KaravasiliLaboratory of Pharmaceutical Technology, Department of Pharmacy, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece

N. Papas, D. Tzetzis, C. KaravasiliDigital Manufacturing and Materials Characterization Laboratory, School of Science and Technology, International Hellenic University, 57001 Thermi, Greece

O.L. Katsamenis, S.K. Robinsonμ-VIS X-Ray Imaging Centre, Faculty of Engineering and Physical Sciences, University road, Highfield campus, Southampton, SO17 1BJ, UKInstitute for Life Sciences, University of Southampton, University road, Highfield campus, Southampton, SO17 1BJ, UK

K. TsongasDepartment of Industrial Engineering and Management, School of Engineering, International Hellenic University, 57001 Thessaloniki, Greece

N. BouropoulosDepartment of Materials Science, University of Patras, 26504 Patras, Greece


 
description: Microfocus Computed Tomography (μ-CT) Data:

Time-lapsed X-ray μCT was used to analyze the internal structure and deployment behavior of the polypill during in vitro hydration in SGF (pH 1.2, 37°C). Imaging was conducted at seven time points (t0 [dry], t1 [15 min], t2 [30 min], t3 [60 min], t4 [120 min], t5 [180 min], t6 [360 min]) using a fast-acquisition protocol to minimize hydration disruption and movement artifacts.

Imaging was performed with Nikon's Med-X prototype μCT scanner (μ-VIS X-ray Imaging Centre, UK). The scanner operated at 100 kVp / 150 μA, with a voxel size ranging from 35 to 47 μm. 901 projections were taken over 360° with an 88 ms exposure per projection, and data were reconstructed into 32-bit volumes using Nikon's CT Agent software.

Morphometry and porosity analysis were conducted in Dragonfly software, with pore space segmented via thresholding. Feret diameter and total volume analyses were performed to assess deployment. Volumetric assessment of the inner and outer layers was conducted at selected time points (t0, t3, t6) using ImageJ and Avizo 3D software, with manual segmentation and automated thresholding for layer differentiation.
description: If you cannot load any of the dragonfly session please try the following: https://theobjectsassist.freshdesk.com/a/solutions/articles/48001079787
description: Content Index List



Video S1.mp4 - a supplementary video related to the experiment or a visualization of the polypill deployment process.

16bit-XCT-reconstructions.zip - An archive containing 16-bit reconstructions from the μCT scans, organised into the following folder structure:



<SAMPLE NAME>/ (Main folder for each sample)



<scan ID>/ (Sub-folder for each scan)<scan file name>/ (Sub-folder containing the reconstructed files)



Reconstructed File: 3D reconstruction file with volume dimensions specified in the filename (16-bit).

.vgi File: Contains metadata and information about the original raw 32-bit reconstruction.



report/



report.pdf: Automatically generated report of each scann detailing the imaging and reconstruction parameters, including scanning settings, reconstruction details, and voxel size. Some commets are ment for X-ray Histology dataset - please ignore.



Scan Settings File: Configuration file detailing the scan settings used during the μCT process.





Analysis.opju - An Origin Project file, which contains data analysis, graphs, and other results from the μCT scans and related experiments.

SKRSegmentation.ORSSession - ORS session file, containing segmentation of the two layers related to the polypill's structure.

DFAnalysis.xlsx - An Excel file, likely containing data analysis, such as volumetric assessments, Feret diameters, or porosity calculations.

projectMasked.ORSSession - ORS session file, related to time-resolved (dynamic) imaging.