DOI: 10.5061/dryad.7rj8k6c
title: Data from: Whole-genome sequences of Malawi cichlids reveal multiple radiations interconnected by gene flow

description: The hundreds of cichlid fish species in Lake Malawi constitute the most
 extensive recent vertebrate adaptive radiation. Here we characterize its
 genomic diversity by sequencing 134 individuals covering 73 species across
 all major lineages. The average sequence divergence between species pairs
 is only 0.1–0.25%. These divergence values overlap diversity within
 species, with 82% of heterozygosity shared between species. Phylogenetic
 analyses suggest that diversification initially proceeded by serial
 branching from a generalist Astatotilapia-like ancestor. However, no
 single species tree adequately represents all species relationships, with
 evidence for substantial gene flow at multiple times. Common signatures of
 selection on visual and oxygen transport genes shared by distantly related
 deep-water species point to both adaptive introgression and independent
 selection. These findings enhance our understanding of genomic processes
 underlying rapid species diversification, and provide a platform for
 future genetic analysis of the Malawi radiation.
description: Amino Acid Alignments
 For Genes in Fig6aThese are the haplotypes
 used to build the haplotype trees showing shared depth adaptation of the
 Diplotaxodon and 'deep benthic' groups. The alignments are in
 the 'fasta' format. Haplotype phase is based on the BEAGLE
 output (not shapeit). Both haplotypes are included per species. The fasta
 headers indicate group assignment (e.g. mbuna), and then the first three
 letters of the genus name and the first three letters of the species
 name.AminoAcidAlignmentsForFig6.tar.gzAll whole-genome variant calls (after BEAGLE genotype refinement)The final product of our variant calling pipeline; obtained as described under: "Variant calling, filtering, and genotype refinement" in Supplementary Methods. This file also includes the A. calliptera samples from Indian Ocean Catchment and outgroup genotypes (N. brichardi), based on a whole genome alignment between the N. brichardi reference and the Lake Malawi M. zebra reference (again described in Supplementary methods).Malinsky_et_al_2018_LakeMalawiCichlids.vcf.gzPhylogenetic treesAll trees linked from Fig 2c, also all SNAPP MCMC samples, and all local Maximum Likelihood trees, both without and with the Indian Ocean (IO) catchment A. calliptera. For details see Methods.phylogenies.tar.gzD statisticsThis table gives values of Patterson’s D(h1, h2, h3, h4) for all combinations of samples of the cichlid species given in Supplementary Table S1 of Malinsky et al. 2018, where the outgroup (h4) is fixed as N. brichardi from Lake Tanganyika. Note that the different geographic variants of A. calliptera were treated separately.D_statistics_allSpeciesTrioCombinations.tsv.gzf statisticsThis table gives values of the f4 admixture ratio f(h1, h2, h3, h4) ([see SOM18 in ref. 31, and fG in ref. 48 in Malinsky et al. 2018] for all combinations of species for which D(h1,h2,h3,h4)&gt;0. The outgroup (h4) is fixed as N. brichardi from Lake Tanganyika. Note that the different geographic variants of A. calliptera were treated separately.f-stats.tsv.gz