cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0fj2gdx
title: CIL: 53322, Drosophila melanogaster, Entire Organism. In Cell Image Library

description: Imaging mode: Gray Scale
Visualization methods: 20x magnification captured by one of the following eyepiece cameras: Swiftcam 3 Megapixel, OMAX 9.0 MP, AmScope MU300
description: 24.9 degrees ℃
description: Video of fruit fly embryonic development. Video filename format is as follows organism_temperature_embryo. Data was collected at 24.9 degrees Celcius
DOI: 10.6075/j0qc02rf
title: CIL:1973, Caenorhabditis elegans. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
DOI: 10.6075/j09z941d
title: CIL:11921, Haemanthus katharinae, endosperm. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: elastic scattering of electrons
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Dividing cells from the liquid endosperm of the lily Haemanthus katherinae were fixed with paraformaldehye, embedded in epoxy, and 0.5 micrometer sections prepared. Images were obtained with the Wisconsin high voltage TEM at 1MEV. For this micrograph of a chromosome at anaphase, the grid was tilted to 60 degrees. A similar micrograph tilted to 40 degrees is grouped with this image, making a stereo pair that provides an oblique 3D view.
DOI: 10.6075/j0xd11b7
title: CIL: 53359, Drosophila melanogaster, Entire Organism. In Cell Image Library

description: 9.4 degrees ℃
description: Imaging mode: Gray Scale
Visualization methods: 20x magnification captured by one of the following eyepiece cameras: Swiftcam 3 Megapixel, OMAX 9.0 MP, AmScope MU300
description: Video of fruit fly embryonic development. Video filename format is as follows organism_temperature_embryo. Data was collected at 9.4 degrees Celcius
DOI: 10.6075/j08w3c0k
title: CIL:11957, Lilium maritimum, pollen mother cell. In Cell Image Library

description: See: S. Inoue 1953. Polarization optical studies of the mitotic spindle. I. The demonstration of spindle fibers in living cells. Chromosoma 5:487-500.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: intrinsic birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: Time lapse movie showing several sequences of mitosis in pollen mother cells of the Easter Lily recorded in 1951 on 16 mm film with an early polarizing microscope developed by Shinya Inoue. The work was instrumental in proving the in vivo reality of the mitotic spindle. The action is sped up by a factor of about 450x.
DOI: 10.6075/j07w69xp
title: CIL:11955. In Cell Image Library

description: Movie of portion of a hypermastigote symbiont from the hindgut of a wood eating cockroach. The rootlets and flagella are birefringent and the beating flagella are clearly seen with the polarizing microscope, used here with compensator.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
DOI: 10.6075/j0mk6bmc
title: CIL:11952, Haemanthus katharinae, endosperm. In Cell Image Library

description: The movie was recorded on 16 mm film in 1956 by A.S. Bajer and J. Mole-Bajer and later transferred to video.
See, S. Inoue and R. Oldenbourg, 1998. Mol Biol Cell 9:1603-1607.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: 16 mm movie copied to video
Data qualification: Processed
description: The time lapse movie shows mitosis in a cell of the liquid endosperm of the African Blood Lily Haemanthus katherinae observed with phase contrast. The movie begins at prophase and proceeds through metaphase, anaphase, telophase, and cell plate formation. The process is speeded up about 300-fold.
DOI: 10.6075/j00z72k6
title: CIL:1195, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
DOI: 10.6075/j0736pkt
title: CIL:38680, Barbulanympha. In Cell Image Library

description: Inoue and Ritter used high-quality sequential coronal polarization (left) and differential interfrence contrast (DIC) microscopy. In the middle column, the focus on the upper surface shows the parting seam in the nuclear envelope (between arrow pairs). The right column shows the median optical section. 
See: Inoué S, Ritter H Jr. (1978). Mitosis in Barbulanympha. II. Dynamics of a two-stage anaphase, nuclear morphogenesis, and cytokinesis.
J Cell Biol. 77:655-84.
description: The series of three vertical columns shows various aspects of mitotic anaphase in a live Barbulanympha (protist). The left column shows the birefringent spindle imaged by polarization microscopy; the center column contains a merge of the polarizing image with differential interference (DIC) microscopy and reveals the chromosomes in a plane near the upper surface of the spindle, and the right hand column, also using merged polarization and DIC, shows the relationship between the spindle and chromosomes in a median optical section of the spindle.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy; differential interference contrast microscopy
Parameter imaged: retardance; boundaries between regions with different refractive index
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Processing history: montage
Data qualification: Processed
DOI: 10.15468/dl.sfsk4t
title: Occurrence Download

description: A dataset containing 13776 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Chaenomeles japonica"
 ]
}

 The dataset includes 13776 records from 422 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009601-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0hx1bx1
title: CIL:1192, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
DOI: 10.6075/j0cr5sg4
title: CIL:11919, Haemanthus katharinae, endosperm. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: elastic scattering of electrons
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Dividing cells from the liquid endosperm of the lily Haemanthus katherinae were fixed with paraformaldehye, embedded in epoxy, and 0.5 micrometer sections prepared. Images were obtained with the Wisconsin high voltage TEM at 1MEV. For this micrograph of a chromosome at anaphase, the grid was tilted to 40 degrees. A similar micrograph tilted to 60 degrees is grouped with this image, making a stereo pair that provides an oblique 3D view.
DOI: 10.6075/j0g44pfs
title: CIL:11918, Haemanthus katharinae, endosperm. In Cell Image Library

description: Dividing cells from the liquid endosperm of the lily Haemanthus katherinae were fixed with paraformaldehye, embedded in epoxy, and 0.5 micrometer sections prepared. Images were obtained with the Wisconsin high voltage TEM at 1MEV. For this micrograph of chromosomes at anaphase, the grid was tilted to 60 degrees. A similar micrograph tilted to 40 degrees is grouped with this image, making a stereo pair that provides an oblique 3D view.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: elastic scattering of electrons
Visualization methods: uranyl salt
Processing history: unprocessed raw data
DOI: 10.6075/j02b8wqq
title: CIL:11940, Sea urchin embryo. In Cell Image Library

description: A live developing sea urchin embryo was imaged using differential contrast microscopy to enhance cellular details. Each minute a through focal series (Z-series) of micrographs was acquired to capture 3D information. In the movie, several Z-series captured about 1 hr apart are presented, providing a '4D' view of this stage in embryo development.
description: 4-D images of a developing sea urchin gastrula (with DIC adjusted to show birefringence of developing spicules). Every 60 seconds, through-focal optical sections were acquired in rapid sequence ( 5 sec/stack of 104 frames) using stepper-motor-driven focusing controls on Shinya Scope 6, controlled by a Universal Imaging MetaMorph program . Out of the whole sequence, which lasted without interruption for 5 hours 20 minutes, four pairs of through-focal sequences separated approximately an hour apart are displayed here.
See Article 42 (S. Inoue and TD Inoue 1986. Ann NY Acad Sci 483:392-404) in Inoue S 2008 Collected Works of Shinya Inoue World Scientific Publishing Co. Ltd. Hackensack, NJ pp 392-404.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Data qualification: Processed
DOI: 10.6075/j0nc600d
title: CIL:11432, Phodopus, endocrine cell. In Cell Image Library

description: Figure 240 from Chapter 7 (Mitochondria) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. The shape of mitochondrial cristae can vary with cell type, shown here in a cell from hamster adrenal cortex. For example, thin folds of the inner membrane are the most common configuration, but they may take on a tubular form (see the end-on profiles indicated by arrows in this figure). Lamellar stacks are common in fetal and neonatal tissues, but may persist in parts of the mitochondrion even in mature cells (see arrow labeled with X). A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
description: Preparation: crosslinking-fixative fixed tissue; embedded tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Data qualification: Processed
DOI: 10.6075/j00p0z38
title: CIL:11934, Rattus. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: mRFP1
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Rat NRK stably expressing RFP targeted to mitochondrial matrix imaged at the M phase of the cell cycle. This image is a z-stack from a living cell collected on a Zeiss 510 LSM confocal using a 63X 1.4NA objective. 
There is a related z-series of a cell in G1/S transition. The stable mito-RFP expressing NRK cell line was prepared by Kasturi Mitra.
DOI: 10.6075/j0639nxq
title: CIL:11933, Rattus. In Cell Image Library

description: Rat NRK stably expressing RFP targeted to mitochondrial matrix imaged at the cell cycle stage G1-S transition. This image is a z-stack from a living cell collected on a Zeiss 510 LSM confocal using a 63X 1.4NA objective. 
There is a related z-series of a cell in M phase. The stable mito-RFP expressing NRK cell line was prepared by Kasturi Mitra.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: mRFP1
Processing history: unprocessed raw data
DOI: 10.6075/j07d2tcc
title: CIL:1193, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
DOI: 10.6075/j0fb51nr
title: CIL:11926, Taricha granulosa, epithelial cell of lung. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine; Cy2
Processing history: background subtraction, 3X3 low pass filter, unsharp mask
Data qualification: Processed;spatialmeasurements;intensitiesquantitation
description: The precise coupling of f-actin and parallel MT movements occurrs in all four zones of f-actin dynamic behavior: the lamellipodium,lamellum, convergence zone, and cell body. Primary cultures of newt (Taricha granulosa) lung epithelial cells were microinjected with X-rhodamine actin and Cy2 tubulin in coinjection. 
Dual-wavelength time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50-mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Excitation wavelength was selected by a filter-wheel apparatus (Sutter Instruments Co.) containing excitation filters for 488 and 568 nm (Chroma) and an opaque disk used as an excitation shutter. Emission was selected by multiple bandpass dichromatic mirror and emission filter (Chroma). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Microscope functions were controlled by MetaMorph software (Universal Imaging). One set of sequential images of the specimen using 488 nm light for Cy2 tubulin and 568 nm light for X-rhodamine f-actin was collected in the ventral focal plane at 10s intervals. Images were processed as follows: (1) background subtraction; (2) 3 x3 low pass filter; (3) unsharp mask filter; (4) color coding MT images green and f-actin images red; and (5) color combining into 24-bit RGB images. Video corresponds to Fig 2 and video 3 in J Cell Biol, 158:31-37, 2002
DOI: 10.6075/j09z93h8
title: CIL:11924, Taricha granulosa, epithelial cell of lung. In Cell Image Library

description: Preparation: living tissue; formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine; Alexa Fluor 488; phalloidin
Processing history: background subtraction, 3X3 low pass filter, unsharp mask
Data qualification: Processed;spatialmeasurements;intensitiesquantitation
description: Comparison of actin speckle microscopy with phalloidin staining. Primary cultures of newt lung epithelial cells were microinjected with X-rhodamine actin. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50-mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Excitation wavelength was selected by a filter-wheel apparatus (Sutter Instruments Co.) containing excitation filters for 488 and 568 nm (Chroma). Emission was selected by multiple bandpass dichromatic mirror and emission filter (Chroma). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10s intervals. Following live cell imaging, cells were fixed with paraformaldehyde, permeabilized and stained with Alexa 488 phalloidin. Images were processed as follows: (1) background subtraction; (2) 3 x3 low pass filter; (3) unsharp mask filter. Video corresponds to Fig 1 and video 1 in J Cell Biol, 158:31-37, 2002.
DOI: 10.6075/j0fq9v82
title: CIL:11938, Taraxacum officinale, pollen grain. In Cell Image Library

description: Preparation: Acid treatment
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: Autofluorescence
Source of contrast: differences in fluorescence emission
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: See: S Inoue and T Inoue (2002) Direct-view high speed confocal scanner: the CSU-10. Meth Cell Biol 70:87-127
description: The movie shows several sequences illustrating the generation of 3D data using the CSU-10 confocal microscope to image the auto-fluorescence of acid treated pollen grains of Dandelion. The first sequence is a 'walk through' in the z direction with the raw images at left, and after unsharp masking on the left. The images in the stack were displaced sidewise sequentially, unsharp masked, summed to generate the views for different angles, and sequences of angular views shown. In these latter sequences, it appears as though 3D images of pollen grains are rotating, providing insight on the complex structure.