cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.13145/bacdive110047.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110048.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110049.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110050.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110051.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110052.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110053.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110054.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110055.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110056.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110057.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110058.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110059.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110060.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110061.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110062.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110063.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110064.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110065.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110066.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110067.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110068.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110069.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110070.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.13145/bacdive110071.20211221.6
title: Streptomyces sp.

description: The range of data encompasses taxonomy, morphology, physiology, sampling and concomitant environmental conditions as well as molecular biology.
DOI: 10.15468/dl.cye7bj
title: Occurrence Download

description: A dataset containing 70 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Kupeantha spathulata (A.P.Davis & Sonké) Cheek"
 ]
}

 The dataset includes 70 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0039144-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.mjcwzk
title: Occurrence Download

description: A dataset containing 3963 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Kleinia neriifolia Haw."
 ]
}

 The dataset includes 3963 records from 82 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0039145-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.9b8ms3
title: Occurrence Download

description: A dataset containing 31 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lecanorchis vietnamica Aver."
 ]
}

 The dataset includes 31 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0039146-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.b3zde3
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ixora mollirama Bremek."
 ]
}

 The dataset includes 4 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0039147-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.vh474y
title: Occurrence Download

description: A dataset containing 154793 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Larix decidua Mill."
 ]
}

 The dataset includes 154793 records from 850 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0039056-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.jkebu9
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Kuramosciadium corydalifolium Pimenov, Kljuykov & Tojibaev"
 ]
}

 The dataset includes 6 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0039148-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.h25z9n
title: Occurrence Download

description: A dataset containing 9733959 species occurrences available in GBIF matching the query:
{
 "Country" : [
 "is Chile"
 ]
}

 The dataset includes 9733959 records from 1958 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0038802-240321170329656/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.35078/u0tfe3/zaamrf
title: Cachoeira Dourada E.jpg

description: :unav
DOI: 10.35078/0ruea6/3zustq
title: Cabeceira dos Fradiques B.jpg

description: :unav
DOI: 10.35078/u0tfe3/oor3rh
title: Lagoa Dourada A.jpg

description: :unav
DOI: 10.35078/0ruea6/monw4a
title: Barreiro VI E.jpg

description: :unav
DOI: 10.35078/0ruea6/xwcrvj
title: Jacu C.jpg

description: :unav
DOI: 10.35078/u0tfe3/lmpme9
title: Paineiras F.jpg

description: :unav
DOI: 10.35078/0ruea6/wfjmmi
title: Córrego das Almas II F.jpg

description: :unav
DOI: 10.35078/u0tfe3/qpvv4y
title: Santa Cruz D.jpg

description: :unav
DOI: 10.35078/0ruea6/rbjkto
title: Pimentas Pov. C.jpg

description: :unav
DOI: 10.35078/u0tfe3/2klnrt
title: Córrego Lagoa Dourada A.jpg

description: :unav
DOI: 10.35078/0ruea6/gcqyp4
title: Furdas II F.jpg

description: :unav
DOI: 10.35078/u0tfe3/gkohqk
title: Leriano Chaves D.jpg

description: :unav
DOI: 10.34740/kaggle/dsv/3455882
title: Mahanagar Yatayat Dataset
DOI: 10.6084/m9.figshare.19151519
title: Supplementary Figure 5_Binding to U373 cells expressing gH monomer

description: Supplementary Figure 5. The gH mAbs recognize conformational epitopes generated during HCMV infection. (a) ARPE-19 cells infected with AD169R (MOI 0.1) and collected 6 dpi were characterized for antibody (2 ug/mL) binding to the surface of infected cells by flow cytometry. (b) U373 gH cells were stained by immunofluorescence (2 ug/mL) and binding was quanti ed by ow cytometry. gH monomer does not traffic to the cellsurface without complexing with gL or UL116 and thus no binding is detected in live cells. The majority of antibody are able to detect intracellular gH in fixed and permeabilized U373-gH cells. Each plot is a representative plot from technical triplicates (n=3) and the experiment was performed in experimental duplicates (n=2).
DOI: 10.6084/m9.figshare.19151519.v1
title: Supplementary Figure 5_Binding to U373 cells expressing gH monomer

description: Supplementary Figure 5. The gH mAbs recognize conformational epitopes generated during HCMV infection. (a) ARPE-19 cells infected with AD169R (MOI 0.1) and collected 6 dpi were characterized for antibody (2 ug/mL) binding to the surface of infected cells by flow cytometry. (b) U373 gH cells were stained by immunofluorescence (2 ug/mL) and binding was quanti ed by ow cytometry. gH monomer does not traffic to the cellsurface without complexing with gL or UL116 and thus no binding is detected in live cells. The majority of antibody are able to detect intracellular gH in fixed and permeabilized U373-gH cells. Each plot is a representative plot from technical triplicates (n=3) and the experiment was performed in experimental duplicates (n=2).
DOI: 10.6084/m9.figshare.19151924
title: Supplementary Figure 7B.pzfx

description: Supplementary Figure 7. anti-gH antibodies limit infection at a post-attachment step. A time of addition assay was performed using ARPE-19 cells incubated with antibody(50 ug/mL) at 0, 30, 60, 120 min post virus addition (MOI 0.2) using the reporter virus AD169R. After 120 min both antibody and virus was removed and replaced with complete media. Relative percentinfection was calculated at 18 hpi and the irrelevant antibody PY102 (anti-IAV) used to normalize infection for each time point.
DOI: 10.6084/m9.figshare.19151924.v1
title: Supplementary Figure 7B.pzfx

description: Supplementary Figure 7. anti-gH antibodies limit infection at a post-attachment step. A time of addition assay was performed using ARPE-19 cells incubated with antibody(50 ug/mL) at 0, 30, 60, 120 min post virus addition (MOI 0.2) using the reporter virus AD169R. After 120 min both antibody and virus was removed and replaced with complete media. Relative percentinfection was calculated at 18 hpi and the irrelevant antibody PY102 (anti-IAV) used to normalize infection for each time point.
DOI: 10.6084/m9.figshare.19163789.v1
title: Sensogram Data for mAb Binding.xlsx

description: The binding measurements of the monoclonal antibody to a gH/gL/UL128/Ul130/UL131a was to determine binding affinity of the mAbs.
DOI: 10.35078/0ruea6/quo0q3
title: Lagoa do São Bento I Pov. D.jpg

description: :unav
DOI: 10.35078/u0tfe3/nthh9f
title: Balduíno B.jpg

description: :unav
DOI: 10.6084/m9.figshare.19142183.v1
title: Supplementary Figure 2B_Neutralization of diverse strains alternative plots

description: Neutralizationcurves of anti-gH antibodies against several HCMV strains in broblasts. Antibody (50-0.016 ug/mL, 5-fold dilutions)neutralization was assessed following infection with diverseHCMV strains (MOI 0.2). The subtle di erences in neutralizationcapacity could be explained by genetic variation in the highlyconserved gH glycoprotein.
DOI: 10.6084/m9.figshare.19152161
title: Supplemental Figure 8a_Ganciclovir mAb Plaques.pzfx

description: Supplementary Figure 8. anti-gH antibodies limit virus dissemination whenused in combination with ganciclovir. (a-c) A summary of HCMV infection andcell-cell spread in ARPE-19 cells infected with AD169R in the presence ofantibody +/- ganciclovir (2.5 uM) based on hydridoma fusion. Images at 7dpi and the plaque number were calculated relative to virus alone controlson each plate. M2E10 and Cytogam were used as negative and positivecontrols respectively. Each condition was performed in triplicate and10,000 um2 was used a cutoff for plaque area.
DOI: 10.6084/m9.figshare.19152161.v1
title: Supplemental Figure 8a_Ganciclovir mAb Plaques.pzfx

description: Supplementary Figure 8. anti-gH antibodies limit virus dissemination whenused in combination with ganciclovir. (a-c) A summary of HCMV infection andcell-cell spread in ARPE-19 cells infected with AD169R in the presence ofantibody +/- ganciclovir (2.5 uM) based on hydridoma fusion. Images at 7dpi and the plaque number were calculated relative to virus alone controlson each plate. M2E10 and Cytogam were used as negative and positivecontrols respectively. Each condition was performed in triplicate and10,000 um2 was used a cutoff for plaque area.
DOI: 10.6084/m9.figshare.19150871.v1
title: Supplementary Figure 3_Neutralization Heat Map.pzfx

description: Supplementary Figure 3. Maximum neutralization of gH antibodies in comparison to Cytogam . The percent neutralization (%) for each antibody was calculated foreach condition where antibody concentration was either 50 ug/mL or 2 ug/mL. Percent neutralization was summarized in a heat map using GraphPad Prism 9 to highlightantibody efficacy at lower concentrations in comparison to Cytogam .
DOI: 10.6084/m9.figshare.19142165
title: Supplementary Figure 11_contour plots for gh mAb binding

description: Supplementary Figure 11. Gating strategy andcontour plots to accompany binding data fromFigure 3b for each antibody (2 ug/mL) when incubatedwith fixed and permeabilized U373 astrocytomacell lines. (a) Representative gating strategyshowing U373-gH/gL cells stained with 5C3, apreviously characterized gH monoclonal, asa positive control for gH expression and binding.(b) A representative contour plot of AF647+ cellsfrom each test condition are shown for eachcell line. All conditions were performed intriplicate. Proportion of AF647+ cells are listedfor each plot.
DOI: 10.6084/m9.figshare.19142183
title: Supplementary Figure 2B_Neutralization of diverse strains alternative plots

description: Neutralizationcurves of anti-gH antibodies against several HCMV strains in broblasts. Antibody (50-0.016 ug/mL, 5-fold dilutions)neutralization was assessed following infection with diverseHCMV strains (MOI 0.2). The subtle di erences in neutralizationcapacity could be explained by genetic variation in the highlyconserved gH glycoprotein.
DOI: 10.6084/m9.figshare.19142174.v1
title: Supplementary Figure 2A_THP1 Neutralization

description: Supplementary Figure 2. Broad neutralization of HCMV gH antibodies by virus strain and cell type. (a) Neutralization in THP-1 cells for anti-gH antibodies using both AD169R (MOI 0.2) and TB40/E (MOI 0.2).
DOI: 10.6084/m9.figshare.19142144.v1
title: Figure 5_Plaque Assay ARPE-19 NHDF

description: <b>Figure 5. Anti-gH antibodies significantly reduce plaque formation in infected cells. </b>The relative number of focus forming units/plaques (&gt;10,000 μm<sup>2</sup>) on Day 7 post infection in both ARPE-19 (a) and NHDF (b) are shown for each antibody at 10 and 0.5 ug/mL using M2E10 (α-IAV) and Cytogam® as negative and positive controls per plate. The % of plaques was determined using single colony verification with 10,000 μm<sup>2</sup> as the minimum colony area based on GFP expression in infected cells. All conditions were repeated using n=3 technical replicates and statistical significance was determined using Ordinary one-way ANOVA with multiple comparisons using Dunnett’s correction with single pooled variance comparing the relative number of plaques at 0.5 ug/mL of gH mAb to plaques in M2E10 treated wells. Statistical significance is denoted as *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ****, p&lt;0.0001. Error bars represent standard deviation from the mean.<b> </b>
DOI: 10.6084/m9.figshare.19150871
title: Supplementary Figure 3_Neutralization Heat Map.pzfx

description: Supplementary Figure 3. Maximum neutralization of gH antibodies in comparison to Cytogam . The percent neutralization (%) for each antibody was calculated foreach condition where antibody concentration was either 50 ug/mL or 2 ug/mL. Percent neutralization was summarized in a heat map using GraphPad Prism 9 to highlightantibody efficacy at lower concentrations in comparison to Cytogam .
DOI: 10.35078/0ruea6/vhi22k
title: Malícia II C.jpg

description: :unav
DOI: 10.6084/m9.figshare.19142129
title: Figure 4B_PepperPRINT Microarray Results

description: Microarray plots for antibodies 1D11, 9A12, 10F8 and 15G11 binding to the overlapping peptide libraries of 7, 10 and 13 aa in length corresponding to the gH. performed by PepperPrint technologies (https://www.pepperprint.com/<b>)</b>
DOI: 10.6084/m9.figshare.19142144
title: Figure 5_Plaque Assay ARPE-19 NHDF

description: <b>Figure 5. Anti-gH antibodies significantly reduce plaque formation in infected cells. </b>The relative number of focus forming units/plaques (&gt;10,000 μm<sup>2</sup>) on Day 7 post infection in both ARPE-19 (a) and NHDF (b) are shown for each antibody at 10 and 0.5 ug/mL using M2E10 (α-IAV) and Cytogam® as negative and positive controls per plate. The % of plaques was determined using single colony verification with 10,000 μm<sup>2</sup> as the minimum colony area based on GFP expression in infected cells. All conditions were repeated using n=3 technical replicates and statistical significance was determined using Ordinary one-way ANOVA with multiple comparisons using Dunnett’s correction with single pooled variance comparing the relative number of plaques at 0.5 ug/mL of gH mAb to plaques in M2E10 treated wells. Statistical significance is denoted as *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ****, p&lt;0.0001. Error bars represent standard deviation from the mean.<b> </b>
DOI: 10.6084/m9.figshare.19142165.v1
title: Supplementary Figure 11_contour plots for gh mAb binding

description: Supplementary Figure 11. Gating strategy andcontour plots to accompany binding data fromFigure 3b for each antibody (2 ug/mL) when incubatedwith fixed and permeabilized U373 astrocytomacell lines. (a) Representative gating strategyshowing U373-gH/gL cells stained with 5C3, apreviously characterized gH monoclonal, asa positive control for gH expression and binding.(b) A representative contour plot of AF647+ cellsfrom each test condition are shown for eachcell line. All conditions were performed intriplicate. Proportion of AF647+ cells are listedfor each plot.
DOI: 10.6084/m9.figshare.19142174
title: Supplementary Figure 2A_THP1 Neutralization

description: Supplementary Figure 2. Broad neutralization of HCMV gH antibodies by virus strain and cell type. (a) Neutralization in THP-1 cells for anti-gH antibodies using both AD169R (MOI 0.2) and TB40/E (MOI 0.2).
DOI: 10.6084/m9.figshare.19142126.v1
title: Figure 4A_Competition

description: <b>Figure 4. The</b> <b>anti-gH antibodies bind two distinct regions of gH. </b>(a)<b> </b>Competition assays were performed with U373 gH/gL cells incubated with increasing concentrations of unlabeled antibody (5-0.1 ug/mL, x-axis) and a constant amount (0.5 ug/mL) of AF647 labeled 5C3 (top), 6E1 (middle), and 10F8 (bottom). The relative MFI of AF647-positive cells is depicted after normalization to the average MFI of labeled antibody incubated with the irrelevant influenza antibody PY102. The full set of antibodies were tested with n=2 technical triplicates and standard deviation from the mean is shown.
DOI: 10.6084/m9.figshare.19142153.v1
title: Figure 6_Ganciclovir Combination with gH mAb panel

description: <b>Figure 6.</b> <b>Anti-gH antibodies with ganciclovir reduces viral plaques. </b>(b) Relative number of plaques at 12 dpi in ARPE-19 cells infected with AD169R cells treated with M2E10, Cytogam®, and Fusion 1, 2, and 3 antibodies +/- ganciclovir. Infection was performed in the presence of antibody at three different concentrations +/- ganciclovir (2.5uM) with n=3 technical replicates per condition. At 12 dpi, from the GFP images, the plaque numbers were calculated relative to M2E10. Each condition was done in triplicate and 10,000 μm<sup>2</sup> was used a cutoff for plaque area. Error bars represent standard deviation from the mean.
DOI: 10.6084/m9.figshare.19151396
title: Supplementary Figure 4_HSV Plaque counts .pzfx

description: Supplementary Figure 4. gH mAbs are specific for HCMV neutralization. Neutralization of HSV-1 US-11 GFP (MOI 0.01) using anti-gH mAbs (50-0.016 ug/mL) and assessed usingGFP as a readout for infection at 24 hpi in Vero cells. Representative images for each condition are shown (a) and the relative number of plaques per condition at 24 hpi arepresented relative to HSV-1 neutralization by the irrelevant in uenza antibody PY102 (b). Heparin (50-0.016 ug/mL) was used as a positive control for each plate and eachcondition was done in triplicate.
DOI: 10.6084/m9.figshare.19151396.v1
title: Supplementary Figure 4_HSV Plaque counts .pzfx

description: Supplementary Figure 4. gH mAbs are specific for HCMV neutralization. Neutralization of HSV-1 US-11 GFP (MOI 0.01) using anti-gH mAbs (50-0.016 ug/mL) and assessed usingGFP as a readout for infection at 24 hpi in Vero cells. Representative images for each condition are shown (a) and the relative number of plaques per condition at 24 hpi arepresented relative to HSV-1 neutralization by the irrelevant in uenza antibody PY102 (b). Heparin (50-0.016 ug/mL) was used as a positive control for each plate and eachcondition was done in triplicate.
DOI: 10.6084/m9.figshare.19142063
title: Figure 3A_Binding to Infected epithelial cells

description: <b>Figure 3. Monoclonal antibodies bind conformational epitopes on gH.</b> (a) ARPE-19 cells were infected with AD169R reporter virus (MOI 0.1) that expresses GFP (x-axis). At 6 dpi cells were trypsinized, fixed and permeabilized for staining with antibodies from Fusion 1-3 (2 ug/mL) and binding was quantified by flow cytometry using anti-mouse AF647 detection antibody (y-axis), n=3 technical triplicates per experiment, n=2 experimental duplicates. Both experimental replicates are provided.