DOI: 10.5061/dryad.n8pk0p2sj
title: Data from: Alkaline phosphatase, lactic dehidrogenase, inflammatory variables and apparent diffusion coefficients from MRI for prediction of chemotherapy response in osteosarcoma

description: Background. This present study aimed to assess if clinical, laboratory and
 MRI were an accurate benchmark in assessing the effectiveness of
 neoadjuvant chemotherapy in osteosarcoma patients. Methods. This was an
 observational analytic study with a cross-sectional design. Research
 subjects were selected using the total sampling method from osteosarcoma
 patients who underwent neoadjuvant chemotherapy during the period between
 January 2017– July 2019. Results.Of the 58 patients included in this
 study, 38 were male and 20 were female aged 5 - 67 years
 (mean,16-year-old. 37(63.8%) patients underwent neoadjuvant chemotherapy
 with CAI regimens and 13 (36.2%) with CA. The tumors were classified as
 stage IIB in 43 (74.1%) patients and stage III in15 (25.9%) patients.
 After undergoing neoadjuvant chemotherapy, 4 patients had poor MSTS, 30
 patients had fair MSTS, 17  and 7 patients had good and excellent
 MSTS score, respectively. Spearman’s test revealed no correlation between
 tumor necrosis after neoadjuvant chemotherapy with a reduction in tumor
 size and MSTS score. Wilcoxon test showed significant differences between
 ALP, ESR, and NLR before and after neoadjuvant chemotherapy in the
 poor-response group. We found no significant difference between LDH and
 LMR before and after neoadjuvant chemotherapy in the good-response group.
 We had 9 patients for ADC value only. No significant statistical
 differences were found in tumor volumes after chemotherapy in both groups.
 Conclusion. We demonstrated that ALP level after neoadjuvant chemotherapy
 was markedly decreased, and was statistically significant in the
 poor-response group. We also demonstrated that LDH value before
 neoadjuvant chemotherapy had a strong correlation with degree of necrosis
 and could be used as a predictive indicator. NLR and LMR cannot be
 independent prognostic factors. MRI plays an important role in evaluating
 tumor volumes and preoperative radiological changes, using DWI and water
 diffusion to predict histological necrosis.    
description: Data Collection
 This was an
 observational analytic study with a cross-sectional design. The study was
 conducted in a tertiary referral hospital, in Jakarta, Indonesia. The
 study had been approved by the ethical committee of our institution with
 Approval No. 0055/ UN2.F1 / ETIK / 2019; and had obtained research
 permission from CMH Research Committee. Research subjects
 were selected using the total sampling method from osteosarcoma patients
 who underwent neoadjuvant chemotherapy during the period between January
 2017 – July 2019. Fifty-eight osteosarcoma patients with the mean age of
 16 years (range was 5-67 years) were included in this study. Informed
 consent was obtained from all the patients or through their parents (in
 the pediatric patients) prior to their enrollment in this study.
 The inclusion
 criteria in the study consisted of patients who were diagnosed with
 osteosarcoma as regards clinicopathological conference (CPC); and who
 underwent 3 cycles of neoadjuvant chemotherapy. They had complete clinical
 evaluation [pain and musculoskeletal tumor society score (MSTS)],
 laboratory and radiologic examinations [magnetic resonance imaging (MRI)
 before and after neoadjuvant chemotherapy)];and histopathological results
 before and after surgery (percentage of tumor necroses as regards Huvos
 grading. Neoadjuvant chemotherapy regimens were adjusted with our
 institution using cisplatin, adriamycin, and iphosphamide (CAI) for
 pediatric patients (&lt; 18 years old) and cisplatin and adriamycin
 (CA) for adult patients. After neoadjuvant
 chemotherapy was completed, a patient would have a limb salvage surgery or
 limb ablation. Surgically removed specimens were sent to the
 musculoskeletal pathologist for evaluation of grading of tumor cell
 necrosis,using the Huvos classification. Huvos grade I was consistent with
 less than 50% necroses; grade II with 50-90% necroses; grade III with more
 than 90-99% necroses; and grade IV with 100% necroses (no viable tumor
 cells). In our study, we grouped the results of the Huvos grading into two
 categories: grade III and IV were described as good-responsegroup while
 grade I and II as poor-response group.   Statistical
 analysis Statistical analysis
 was performed using Mann-Whitney U test for the unpaired group and
 Wilcoxon test for variables in the paired group. Spearmen’s test was used
 to correlate between the variables. All statistical analyses were
 performed using SPSS software version 23.0. A p value of&lt;0.05 was
 considered statistically significant.