cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9956840.v1
title: Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells

description: Abstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation.
DOI: 10.6084/m9.figshare.9956825
title: Parasite load, iNOS and cytokine profiles, and histopathological aspects of Leishmania infantum infection in dogs with different clinical presentations

description: ABSTRACT: Visceral leishmaniasis (VL) is a zoonotic disease with a canine urban reservoir in South America. Dogs from an endemic area within Brazil, which were naturally infected with Leishmania infantum, and those presenting severe clinical (SC), mild, or no clinical (MNC) disease, were evaluated. Parasite load, histopathology, and cytokine and iNOS mRNA expressions were assessed in the spleen and liver in order to determine the potential markers for disease susceptibility or resistance. As a result, dogs with both SC and MNC had high parasite loads; IFN-γ was the most expressive cytokine in both organs, along with IL-6 and IL-4 being detected in the spleen and liver, and IL-10 only in liver. The hepatic tissue presented higher medians for IFN-γ and IL-10, and was the main organ to produce cytokines with hepatic IL-10 suggesting a regulatory follow up. Granulomas were detected in both organs; however, when absent in spleen, they were associated with elevated IL-6 levels, thus highlighting the anti-inflammatory role of IL-6. Microscopic lesions in the spleen were predominantly characterized by an extensively disorganized white pulp and splenic response was suggested as sub optimized. Parasite load, tissue damage, and immunological response may vary in the dogs with similar clinical symptoms, which may not be a good parameter for assessing the animal’s susceptibility to VL.
DOI: 10.6084/m9.figshare.9956819
title: Interfacial Properties and Bottom/Top Hardness Ratio Produced by Bulk Fill Composites in Dentin Cavities

description: Abstract The aim of this study was to evaluate the bond strength, bottom/top hardness ratio, marginal adaptation, and interfacial nanoleakage of regular viscosity bulk fill composites (RVBFC) and regular viscosity traditional composites (RVTC). Two RVBFC (Filtek Bulk Fill and Aura Bulk Fill) and two RVTC (Filtek Z250XT and Aura) were assessed. Forty conical cavities (4.8×2.8×4.0) were prepared in bovine dentin and restored with composites (n=10). After 24h in water, marginal adaptation was evaluated by staining with a caries detector. The top and bottom surfaces of the conical restorations were stained for five seconds and the gap percentage in the composite/dentin interface was determined using digital images on a measurement program (ImageTool). The Vickers microhardness was measured and the bottom/top microhardness ratio (B/T) was determined. Push-out bond strength test was performed in a universal testing machine (0.5mm/min) and failure modes were evaluated in a stereomicroscope (20×). Other specimens (n=3) were produced for interfacial nanoleakage evaluation. Data were analyzed using one and two-way ANOVA and Tukey’s test (α=0.05). The gap percentage was higher in the bottom compared to the top. The B/T ratio of the Aura Bulk Fill was statistically lower than other composites. Push-out bond strength were similar among composites. The RVBFC presented lower nanoleakage than the RVTC in the bottom of the conical restoration and there was no difference among the materials in the top surfaces. In conclusion, Filtek Bulk Fill performed better than Aura Bulk Fill regarding the analyzed properties.
DOI: 10.6084/m9.figshare.9956825.v1
title: Parasite load, iNOS and cytokine profiles, and histopathological aspects of Leishmania infantum infection in dogs with different clinical presentations

description: ABSTRACT: Visceral leishmaniasis (VL) is a zoonotic disease with a canine urban reservoir in South America. Dogs from an endemic area within Brazil, which were naturally infected with Leishmania infantum, and those presenting severe clinical (SC), mild, or no clinical (MNC) disease, were evaluated. Parasite load, histopathology, and cytokine and iNOS mRNA expressions were assessed in the spleen and liver in order to determine the potential markers for disease susceptibility or resistance. As a result, dogs with both SC and MNC had high parasite loads; IFN-γ was the most expressive cytokine in both organs, along with IL-6 and IL-4 being detected in the spleen and liver, and IL-10 only in liver. The hepatic tissue presented higher medians for IFN-γ and IL-10, and was the main organ to produce cytokines with hepatic IL-10 suggesting a regulatory follow up. Granulomas were detected in both organs; however, when absent in spleen, they were associated with elevated IL-6 levels, thus highlighting the anti-inflammatory role of IL-6. Microscopic lesions in the spleen were predominantly characterized by an extensively disorganized white pulp and splenic response was suggested as sub optimized. Parasite load, tissue damage, and immunological response may vary in the dogs with similar clinical symptoms, which may not be a good parameter for assessing the animal’s susceptibility to VL.
DOI: 10.6084/m9.figshare.9956813
title: Considerations on design and implementation parameters of domestic wastewater treatment by subsurface flow constructed wetlands

description: ABSTRACT Constructed wetlands might be an alternative for communities away from urban centers and not served by a domestic wastewater treatment system. The purpose of this study was to provide instructions for the implementation of subsurface flow constructed wetland systems. To that end, we gathered information regarding the construction aspect, plants, and operational parameters used in systems which already operate in the country and the respective efficiency of these sets after previous treatment. The system in real scale proposed by Oliveira et al. (2005) was prominent among those that presented the highest efficiency. It was preceded by upflow anaerobic reactor built in brick, with macrophyte of the Typha genre, crushed stone at the entry and exit of the system, and sand in its intermediate portion. It required 1.04 m² surface area per inhabitant in humid temperate climate and hot summer, 1.71 m³ d-1 flow, and one-day hydraulic detention. The considerations presented here might help the construction of this kind of system, regarding dimensional and operational criteria.
DOI: 10.6084/m9.figshare.9956816
title: Comparison of two soft tissue substitutes for the treatment of gingival recession defects: an animal histological study

description: Abstract Objectives: This study aimed to compare two different soft tissue replacement grafts in their ability to treat gingival recession defects and successfully integrate with the surrounding tissues. Methodology: Nine beagle dogs were included and followed up to 10 weeks. Sites for intervention were allocated to one of the grafting materials investigated. Treatment consisted of coronally advanced flap combined with one of the two soft tissue substitutes on a previous surgically created defect. Materials employed were porcine-derived acellular dermal matrix (ADM) [Novomatrix™ (Test)] and collagen-based matrix (CBM) [Mucograft® (Control)]. Animals were sacrificed at 2, 6, and 10 weeks postoperatively and compared using descriptive histology and histomorphometric outcomes. Results: Macroscopic findings were similar between test and control groups at all intervals. After 10 weeks, both groups demonstrated successful incorporation of the grafting materials without signs of rejection and with comparable tissue integration. The histomorphometric data were similar between groups at 2 weeks; however, the test group provided greater root coverage and increase in tissue thickness than the control at 6- and 10-weeks post surgically. Conclusions: Both porcine-derived ADM and CBM revealed similar histological outcomes with successful integration and absence of adverse events. Test group provided superior outcomes regarding root coverage and increase in tissue thickness.
DOI: 10.6084/m9.figshare.9956819.v1
title: Interfacial Properties and Bottom/Top Hardness Ratio Produced by Bulk Fill Composites in Dentin Cavities

description: Abstract The aim of this study was to evaluate the bond strength, bottom/top hardness ratio, marginal adaptation, and interfacial nanoleakage of regular viscosity bulk fill composites (RVBFC) and regular viscosity traditional composites (RVTC). Two RVBFC (Filtek Bulk Fill and Aura Bulk Fill) and two RVTC (Filtek Z250XT and Aura) were assessed. Forty conical cavities (4.8×2.8×4.0) were prepared in bovine dentin and restored with composites (n=10). After 24h in water, marginal adaptation was evaluated by staining with a caries detector. The top and bottom surfaces of the conical restorations were stained for five seconds and the gap percentage in the composite/dentin interface was determined using digital images on a measurement program (ImageTool). The Vickers microhardness was measured and the bottom/top microhardness ratio (B/T) was determined. Push-out bond strength test was performed in a universal testing machine (0.5mm/min) and failure modes were evaluated in a stereomicroscope (20×). Other specimens (n=3) were produced for interfacial nanoleakage evaluation. Data were analyzed using one and two-way ANOVA and Tukey’s test (α=0.05). The gap percentage was higher in the bottom compared to the top. The B/T ratio of the Aura Bulk Fill was statistically lower than other composites. Push-out bond strength were similar among composites. The RVBFC presented lower nanoleakage than the RVTC in the bottom of the conical restoration and there was no difference among the materials in the top surfaces. In conclusion, Filtek Bulk Fill performed better than Aura Bulk Fill regarding the analyzed properties.
DOI: 10.6084/m9.figshare.9956816.v1
title: Comparison of two soft tissue substitutes for the treatment of gingival recession defects: an animal histological study

description: Abstract Objectives: This study aimed to compare two different soft tissue replacement grafts in their ability to treat gingival recession defects and successfully integrate with the surrounding tissues. Methodology: Nine beagle dogs were included and followed up to 10 weeks. Sites for intervention were allocated to one of the grafting materials investigated. Treatment consisted of coronally advanced flap combined with one of the two soft tissue substitutes on a previous surgically created defect. Materials employed were porcine-derived acellular dermal matrix (ADM) [Novomatrix™ (Test)] and collagen-based matrix (CBM) [Mucograft® (Control)]. Animals were sacrificed at 2, 6, and 10 weeks postoperatively and compared using descriptive histology and histomorphometric outcomes. Results: Macroscopic findings were similar between test and control groups at all intervals. After 10 weeks, both groups demonstrated successful incorporation of the grafting materials without signs of rejection and with comparable tissue integration. The histomorphometric data were similar between groups at 2 weeks; however, the test group provided greater root coverage and increase in tissue thickness than the control at 6- and 10-weeks post surgically. Conclusions: Both porcine-derived ADM and CBM revealed similar histological outcomes with successful integration and absence of adverse events. Test group provided superior outcomes regarding root coverage and increase in tissue thickness.
DOI: 10.6084/m9.figshare.9956807
title: HEART AT RISK: ELECTRONIC EDUCATIONAL GAME WITH INFORMATION ON DOPING IN ATHLETES

description: ABSTRACT Introduction Games are recognized tools that can be used for teaching, awareness-building and behavioral change. In the world of competitive sports, it is necessary to both educate athletes and make them aware that doping is an unsportsmanlike practice, particularly because this conduct can be life-threatening. A game called “Heart at Risk” was developed in this context for the purpose of teaching athletes which substances are characterized as doping by the World Antidoping Agency, and to raise their awareness of health hazards through entertainment. Objective Present and empirically evaluate the efficacy of the game as a tool for educating athletes on substances characterized as doping. Methods The sample group was formed by 20 players in the under-20 category of Clube do Remo (Rowing Club), aged over 18 years. The athletes underwent a pre-test, played the Heart at Risk game, and took a post-test at the end. Both tests were related to healthy sport practices (positive factors) and to doping substances (negative factors). Results Regarding the knowledge of positive factors, there was an increase of knowledge in relation to low doses of caffeine (∆ = 40%; p-value= 0.0125) and doping assessment (∆ = 55%; p-value = 0.0017). Regarding negative factors, there was an increase of knowledge in relation to diuretics (∆ = 25%; p-value = 0.0455), contaminated thermogenic (∆ = 35%; p-value = 0.0191), growth hormone-GH (∆ = 50%; p-value = 0.0025), contaminated supplements (∆ = 65%; p-value = 0.0007), and testosterone (∆ = 55%; p-value = 0.0017). Conclusion The Heart at Risk game proved to be an effective resource in the process of learning about prohibited substances and positive factors for sports performance. Level of Evidence IV; Case series.
DOI: 10.6084/m9.figshare.9956810
title: Preferences and demographic parameters of cowpea aphid on advanced lines of semiprostrate cowpea

description: Abstract: The objective of this work was to determine the preference and the demographic parameters of cowpea aphid (Aphis craccivora) in advanced lines of semiprostrate cowpea (Vigna unguiculata), and to verify both the existance and type of plant resistance to this insect attack. Aphid preference to 20 cowpea genotypes was tested in a randomized complete block design, with six replicates. Lines showing the highest levels of resistance and susceptibility in the preference tests were selected for use in the demographic parameter tests. Based on aphid birth rate and mortality, fertility life tables were constructed. The MNCO4-792F-123 line was classified as resistant. The lowest values for cowpea aphid intrinsic and finite growth rates were obtained for the MNCO4-792F-123, and the highest ones for MNCO4-782F-108 and 'BRS Novaera' genotypes, whose levels exceeded those of 'Vita 7', the standard genotype for susceptibility. The MNCO4-792F-123 line is the least favorable for the development of cowpea aphid, while MNCO4-782F-108 and 'BRS Novaera' are the most susceptible ones.
DOI: 10.6084/m9.figshare.9956813.v1
title: Considerations on design and implementation parameters of domestic wastewater treatment by subsurface flow constructed wetlands

description: ABSTRACT Constructed wetlands might be an alternative for communities away from urban centers and not served by a domestic wastewater treatment system. The purpose of this study was to provide instructions for the implementation of subsurface flow constructed wetland systems. To that end, we gathered information regarding the construction aspect, plants, and operational parameters used in systems which already operate in the country and the respective efficiency of these sets after previous treatment. The system in real scale proposed by Oliveira et al. (2005) was prominent among those that presented the highest efficiency. It was preceded by upflow anaerobic reactor built in brick, with macrophyte of the Typha genre, crushed stone at the entry and exit of the system, and sand in its intermediate portion. It required 1.04 m² surface area per inhabitant in humid temperate climate and hot summer, 1.71 m³ d-1 flow, and one-day hydraulic detention. The considerations presented here might help the construction of this kind of system, regarding dimensional and operational criteria.
DOI: 10.6084/m9.figshare.9956801
title: Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

description: ABSTRACT: Cassava (Manihot esculenta Crantz) is an important crop in Brazil and Pará is the major producer of roots. High temperature and humidity of tropical regions favor the development of various diseases, among them the cassava root rot. The objective of this study was to evaluate the effect of luminosity and culture medium on the mycelial growth and sporulation of Phytopythium sp. associated with different methods of inoculation on cassava roots. In vitro tests for pathogen growth were established in a 2 x 6 factorial design (luminosity x culture medium) with five replicates and the means were compared by t test (P≤0.05). The culture medium containing sweet cassava root produced greater mycelial development and higher pathogen sporulation and it was the most suitable medium for pathogen culture. The culture under absence of light generated better mycelial growth than culture under 12 hour of light. Regarding the type of inoculation, the response was better when deeper injuries were induced.
DOI: 10.6084/m9.figshare.9956798
title: Children's needs with congenital syndrome related to Zika virus in the domiciliary context

description: Abstract Background Families of children with Zika virus have faced several demands related to child care, at home and in social life. Objective To understand family dynamics and the promotion of care for children with Congenital Zika Virus Syndrome in the home context. Method Qualitative study, in which eight families of children with Congenital Zika Virus Syndrome participated. Semi-structured interviews were conducted through home visits. Results Three categories emerged that portray home care and the challenges faced by families: needs of children in the home environment; family demands; care coordination. Child-centered problems were identified, such as difficulty in swallowing, sleep and rest, and development, family-centered problems such as caregiver burden and fragmentation of care in the health network. Conclusion The care of children with Zika virus-related microcephaly needs to be extended to a family-centered care, based on a coordinated and integrated health network based on Primary care.
DOI: 10.6084/m9.figshare.9956795
title: Introduction to socio-ecological analysis based on the collective mapping of someone’s own territory: uses made by different social actors and their associated conflicts

description: Abstract: The present article exposes a practice in the course “Ecology and Development” in a school of natural sciences. The objective is to promote the students’ appropriation of the campus space, the socio-environmental conflicts, and the concepts of socio-ecological systems. Through discussion and the use of aerial images, bibliographical information, journalistic notes, photographs and the narration of a member of the University Campus Community Plant Nursery, landscape changes and its uses are historically reconstructed. The work has led to the identification of different actors involved, a spatial visualization of the uses they make of space, and the identification of conflicts. This didactic and technical tool has proven to be a valuable device to facilitate dialogue among different actors.
DOI: 10.6084/m9.figshare.9956798.v1
title: Children's needs with congenital syndrome related to Zika virus in the domiciliary context

description: Abstract Background Families of children with Zika virus have faced several demands related to child care, at home and in social life. Objective To understand family dynamics and the promotion of care for children with Congenital Zika Virus Syndrome in the home context. Method Qualitative study, in which eight families of children with Congenital Zika Virus Syndrome participated. Semi-structured interviews were conducted through home visits. Results Three categories emerged that portray home care and the challenges faced by families: needs of children in the home environment; family demands; care coordination. Child-centered problems were identified, such as difficulty in swallowing, sleep and rest, and development, family-centered problems such as caregiver burden and fragmentation of care in the health network. Conclusion The care of children with Zika virus-related microcephaly needs to be extended to a family-centered care, based on a coordinated and integrated health network based on Primary care.
DOI: 10.6084/m9.figshare.9956807.v1
title: HEART AT RISK: ELECTRONIC EDUCATIONAL GAME WITH INFORMATION ON DOPING IN ATHLETES

description: ABSTRACT Introduction Games are recognized tools that can be used for teaching, awareness-building and behavioral change. In the world of competitive sports, it is necessary to both educate athletes and make them aware that doping is an unsportsmanlike practice, particularly because this conduct can be life-threatening. A game called “Heart at Risk” was developed in this context for the purpose of teaching athletes which substances are characterized as doping by the World Antidoping Agency, and to raise their awareness of health hazards through entertainment. Objective Present and empirically evaluate the efficacy of the game as a tool for educating athletes on substances characterized as doping. Methods The sample group was formed by 20 players in the under-20 category of Clube do Remo (Rowing Club), aged over 18 years. The athletes underwent a pre-test, played the Heart at Risk game, and took a post-test at the end. Both tests were related to healthy sport practices (positive factors) and to doping substances (negative factors). Results Regarding the knowledge of positive factors, there was an increase of knowledge in relation to low doses of caffeine (∆ = 40%; p-value= 0.0125) and doping assessment (∆ = 55%; p-value = 0.0017). Regarding negative factors, there was an increase of knowledge in relation to diuretics (∆ = 25%; p-value = 0.0455), contaminated thermogenic (∆ = 35%; p-value = 0.0191), growth hormone-GH (∆ = 50%; p-value = 0.0025), contaminated supplements (∆ = 65%; p-value = 0.0007), and testosterone (∆ = 55%; p-value = 0.0017). Conclusion The Heart at Risk game proved to be an effective resource in the process of learning about prohibited substances and positive factors for sports performance. Level of Evidence IV; Case series.