cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2011.166
title: Oxidative stability of tuna oil emulsion coated with polyelectrolyte multilayer

description: The aim of this study is to retard the oxidation of unsaturated fatty acid in tuna oil-in-water emulsion by preparing as multilayer emulsion and adding antioxidants. The multilayer tuna oil-in-water emulsions were prepared using layer-by-layer technique. The primary emulsion was prepared by homogenizing 5 %w/v tuna oil, 0.4 %w/v WPI in 100 mM acetic-acetate buffer solution as emulsifier. The secondary and tertiary emulsions were prepared by coating the oil droplets with the charged biopolymers. Chitosan, a cationic polyelectrolyte, and alginate and carrageenan, anionic polyelectrolytes, were selected. The pH of emulsifier was controlled at either pH 3.5 and 6.0. The physical characteristic and physical stability was investigated by measuring the creaming index, % transmission, particle size and zeta potential. The particle size of emulsions increased, while the creaming index and % transmission were improved when increasing the number of layers around emulsion. The physical stability of tertiary emulsions was improved comparing to the primary and secondary emulsions for both pH conditions. The oxidative stability of emulsions was investigated via the ferric thiocyanate method by using UV-Vis spectroscopy. After storage, all emulsions released lipid hydroperoxide. We found that the secondary and tertiary emulsions decreased the oxidation reaction comparing to the primary emulsion. Thus, the multilayer emulsions could be improved the stability of tuna oil-in-water emulsion. The effect of antioxidants, sesame oil, capsaicin oleoresin, genistein and roselle extract on the decrease in the oxidation rate of primary emulsion were investigated. Sesame oil and capsaicin oleoresin were added directly in tuna oil, which we found that added 0.5 %w/v capsaicin oleoresin the better oxidative stability. While genistein and roselle extract were added directly in emulsifier solutions, we found that the 200 ppm roselle extract could improve the oxidative and physical stabilities.
DOI: 10.14457/cu.the.2011.167
title: Synthesis and characterization of porphyrin-based metal-organic frameworks for gas adsorption

description: In this research, synthesis of porphyrinic linkers, porphyrin-based Metal-Organic Frameworks construction and their physical properties were concerned. For the first one, four porphyrin derivatives, including dicarboxyphenylporphyrin, 1,4-phenylene-bridged meso–meso linked diporphyrin, meso-meso β-β β-β triply linked (fused) diporphyrin and tetracarboxybiphenylporphyrin were synthesized. However, only the last porphyrin derivative was used as organic linker for MOFs synthesis in the presence of M(NO3)2 (M= Zn, Cu) with or without 4,4’-bipyridine (as pillar) by using dimethylformamide as solvent. The obtained porphyrin-based MOFs were investigated their morphologies and gas adsorption properties for the potential use in gas storage. Morphology of synthesized MOFs exhibited as micro-particles of MOFs in various forms, such as cubic, rod and platelet shapes. The nitrogen adsorption-desorption isotherms performed as type I adsorption isotherm which was typical for microporous materials. Total specific surface areas were in range 400-600 m2/g and the quite narrow pore size distribution was found with the average pore diameter of 0.6 nm. Moreover, the stability of this framework was investigated by TGA with the temperature not over than 300°C. It should be concluded that the synthesized MOFs via pathway B, using M-TCBPP as organic linkers with 4,4’-bipyridine as a pillar between the porphyrin plane, was the suitable pathway to synthesize MOFs.
DOI: 10.14457/cu.the.2011.168
title: Physical properties of mma grafted natural rubber film reinforced with in situ silica

description: The improvement in the compatibility and adhesion between natural rubber (NR) grafted with methylmethacrylate (MMA) and silica by two types of vinylated silane, γ-methacryloxypropyltrimethoxysilane (γ-MPS) and vinyltriethoxysilane (VTES) as a coupling agent was reported. In the first step, the NR latex (30%DRC) was grafted with MMA monomer via emulsion polymerization using cumene hydroperoxide/tetraethylene pentamine (CHPO/TEPA) redox system as an initiator. The latex core and PMMA shell feature was found by transmission electron microscopic (TEM) analysis. Graft copolymerization of NR-g-MMA with -MPS or VTES was then proceeded. To generate silica in the grafted NR latex, tetraethoxysilane (TEOS) was mixed with the latex mixture. The composite latex particles appeared to be a core-shell structure with the silica sol forming encompassing the NR core. The silica particles were well dispersed in the rubber matrix as observed by SEM. The conversions of -MPS-TEOS and VTES-TEOS to silica particles in the composite were between 60 to 90%. The results of mechanical test suggested that the vinyl group on the in situ generated silica played an important role in the reinforcement of rubber films.
DOI: 10.14457/cu.the.2011.169
title: Stability of conditional frechet functional equation

description: In this dissertation, we will first prove the Hyers-Ulam stability of the Fréchet functional equation, and then we will prove such stability when the span is restricted.
DOI: 10.14457/cu.the.2011.170
title: Changes in anatomical structure and cell wall metabolism of 'Hom Thong' Banana peel following hot water treatment

description: Fruit softening is an important change during the ripening process that affects fruit quality and postharvest shelf life. Cell wall degrading enzymes are considered to be the main factor responsible for cell wall disassembly and texture changes in fruit. To elucidate the effect of hot water treatment (HWT, 50 oC, 10 min) on the softening of ‘Hom Thong’ banana (Musa acuminata, AAA group), firmness, cell wall composition, the activities of cell wall degrading enzymes, and the expression of related genes were investigated in the peel. The decrease in firmness was delayed in hot water treated fruit as compare to control fruit during storage at 25 oC. Application of hot water could delay the increase of water-soluble pectin correlated to fruit firmness during storage. A higher level of HCl-soluble pectin was detected in treated fruits, indicating the reduction of cell wall polymer solubilization. Loss of hemicellulose was also slowed down in treated fruits. HWT did not affect the amount of EDTA-soluble pectin and cellulose. For enzyme activity and gene expression, treatment of intact fruit with hot water reduced activities of pectate lyase (PL) and β-galactosidase (β-Gal) in the peel, and also decreased the accumulation of their mRNAs (MaPL1 and MaGAL, respectively). Polygalacturonase (PG) enzyme activity was inhibited by heat treatment, and the increase in MaPG1 expression was delayed. HWT had little effect on pectin methylesterase (PME) or β-1,3 glucanase (Glu). After treatment, reduced PME activity was measured, but activity returned to levels similar to that of control fruits. PME activity varied during storage and did not correlate well with MaPME1 expression. There was no significant difference in Glu enzyme activity or MaGLU expression between treated and control fruit. Further investigation in cell wall structural changes following HWT through microscopic observation showed that structural alterations in peel of hot water treated banana fruits being slower as compared to untreated fruits. The reduction of peel thickness and number of cell layer occurred during storage was retarded by exposure to hot water. The formation of air space in ground tissue of hot water treated fruit was slower than that of control fruits. These results suggest that postharvest HWT was effective in preventing cell wall degradation of ‘Hom Thong’ banana fruit and reduced activities and gene expression of some enzymes associated with cell wall modification leading to maintainence of firmness in ‘Hom Thong’ banana.
DOI: 10.5517/ccdc.csd.cc1nq7tf
title: CCDC 1540725: Experimental Crystal Structure Determination

description: Related Article: L. Singla, H.R. Yadav, A. Roy Choudhury|2020|Acta Crystallogr.,Sect.B:Struct.Sci.,Cryst.Eng. and Mat.|76|604|doi:10.1107/S2052520620006873
DOI: 10.5517/ccdc.csd.cc1nq80n
title: CCDC 1540731: Experimental Crystal Structure Determination

description: Related Article: L. Singla, H.R. Yadav, A. Roy Choudhury|2020|Acta Crystallogr.,Sect.B:Struct.Sci.,Cryst.Eng. and Mat.|76|604|doi:10.1107/S2052520620006873
DOI: 10.14457/cu.the.2011.171
title: Determination of DNA sequences following dot blot hybridization format employing filter paper immobilized with quaternized polymer brushes and peptide nucleic acid

description: In this research, a new paper-based platform for colorimetric detection of specific DNA sequences employing a new conformationally constrained pyrrolidinyl peptide nucleic acid (acpcPNA) as a probe has been developed. The filter paper was modified to be positively charged with grafted polymer brushes of QPDMAEMA to be used for selective capturing of the negatively charged DNA, but not the neutral acpcPNA, by electrostatic interacton. Only whe the sequences of the DNA and modifier labeled acpePNA probe (m-PNA) are complementray, the probe can be immobilized through hybridization with the surface-bound DNA. The presence of DNA-PNA hybrids can be detected by colorimetric assay via either enzymatic or polymerization amplificaton. In enzymatic amplification mode, it is obvious that this assay was capable of discriminating a single base mismatch at a detection limit of at least 10 fmole. Moreover, the QPDMAEMA grafted filter paper exhibited a superior performance to the commercial membranes, nylon 66 and nitrocellulose. In polymerization amplification mode, it was demonstrated that the desired red copolymer of HEMA-Rh B and PEGMA grew from the initiator adsorbed on paper with minimal background from non-specific adsorption of unbound copolymer. However, the signal amplification of PNA-DNA hybridization in picomol level was not observed by naked eyes because concurrent ARGET ATRP/RAFT for this assay required initiator with a mount greater than 1 nmol.
DOI: 10.14457/cu.the.2011.172
title: Effect of autonomic neuropathy on forearm blood flow in type 2 diabetes

description: ภาวะความผิดปกติทางระบบประสาทออโตโนมิกของระบบหัวใจและหลอดเลือด (cardiovascular autonomic neuropathy, CAN) เป็นภาวะแทรกซ้อนที่พบได้บ่อยในผู้ป่วยเบาหวาน โดยอาจส่งผลให้เกิดความผิดปกติในการควบคุมความตึงตัว และการตอบสนองของหลอดเลือดทั่วร่างกาย วัตถุประสงค์ : เพื่อศึกษาว่าการเปลี่ยนแปลงของระบบประสาทออโตโนมิกในการควบคุมความตึงตัวของหลอดเลือดนั้นจะมีผลต่อการไหลเวียนเลือด บริเวณปลายแขนในขณะ reactive hyperemia (maxFBF) และภายหลังการออกกำลังกายหรือไม่ในผู้ป่วยเบาหวาน ระเบียบวิธีวิจัย : ในงานวิจัยนี้ ผู้ป่วยเบาหวานจะถูกแบ่งออกเป็น กลุ่มที่มี และไม่มีความผิดปกติของ CAN โดยแบ่งได้จากการตอบสนองของระบบหัวใจและหลอดเลือดต่อการทดสอบของ Ewing et al. 5 ขั้นตอน และได้มีการวัดอัตราการไหลเวียนเลือดบริเวณปลายแขนโดยใช้ venous occlusion plethysmograph ในภาวะ reactive hyperemia และ เมื่อออกกำลังกายเป็นจังหวะโดยใช้ handgrip exercise ที่ความหนัก 45% ของความสามารถสูงสุดที่ทำได้ รวมทั้งได้มีการวัดการไหลเวียนเลือดในกลุ่มควบคุมที่มีสุขภาพดีด้วยเช่นกัน ผลการทดสอบ : ในผู้ที่มีสุขภาพดีจำนวน 33 คน และในผู้ป่วยเบาหวาน จำนวน 57 คน (ชาย 24 คน, หญิง 33 คน อายุระหว่าง 40-75 ปี) แบ่งเป็น ผู้ป่วยที่มี CAN จำนวน 21 ราย และไม่มี CAN จำนวน 36 ราย พบว่า maxFBF ในผู้ป่วยที่มี CAN มีค่าสูงกว่าผู้ป่วยที่ไม่มี CAN อย่างมีนัยสำคัญทางสถิติ (27.71 ± 6.94 vs. 21.56 ± 4.17 ml/100ml/min, P=0.001) นอกจากนี้ผู้ป่วยที่มี CAN จะมีค่าความสามารถในการนำเลือดของหลอดเลือดบริเวณแขนมากกว่า (FVC, P=0.012) และความต้านทานการไหลเวียนเลือดน้อยกว่า (FVR, P=0.011) เมื่อเทียบกับผู้ป่วยที่ไม่มี CAN แต่ในผู้ที่มีสุขภาพดีจะมีค่า maxFBF, FVC มากที่สุด และ FVR น้อยที่สุด อย่างไรก็ตาม การกระตุ้นด้วย handgrip exercise นั้นยังไม่ทำให้เกิดความแตกต่างของการตอบสนองของหลอดเลือดอย่างมีนัยสำคัญ สรุปผลการทดลอง : ผู้ป่วยเบาหวานที่มี CAN มีการปรับเปลี่ยนการควบคุมความต้านของหลอดเลือดส่วนปลาย ซึ่งช่วยให้มีการขยายตัวมากขึ้น โดยมีค่า maxFBF เพิ่มสูงขึ้น เพราะเป็นไปได้ว่าในภาวะ CAN อาจมีกระแสประสาทซิมพาเทติกมาที่หลอดเลือดลดลง หรือมีการตอบสนองที่มากกว่าปกติต่อการกระตุ้นที่กล้ามเนื้อเรียบบริเวณหลอดเลือดให้เพิ่มการขยายตัวของหลอดเลือด
DOI: 10.14457/cu.the.2011.173
title: Development of asiatic acid solid lipid nanoparticles for nasal delivery

description: The purpose of this study was to prepare asiatic acid solid lipid nanoparticles (AA-SLN) for nasal delivery to brain. The SLN were preliminary prepared by hot high-pressure homogenization method composed of six solid lipids referred to trimyristin (TM), tistearin (TS), glyceryl behenate (GB), hydrogenated palm oil (SOF), palmitic acid (PA) and stearic acid (SA). Tween80 (TW80) was used as surfactant and poloxamer 188 (PL188), span80 (SP80), span85(SP85) and solutol HS15 (SL) were used as co-surfactants. After storage at accelerated condition, 25 formulations were maintained stabilized products. Palmitic acid and stearic acid could not produced stabilized formulations at all. Then asiatic acid was loaded SLN (AA-SLN) were prepared to determine physicochemical properties and stability at 4๐ C, room temperature and 45๐ C for 6 months. The particle size range from 34.53 + 0.81 to 186.50 + 2.23 nm. Larger sizes were obtained after coating with by chitosan. The zeta potential showed slightly negative charged and tended to slightly positive charged after coating with chitosan. The pH values were in the range of 4.5-6.5 which are suitable for used in nasal delivery route, they were slightly decreased when coating with chitosan. The entrapment efficiency of AA-SLN demonstrated high % entrapment efficiency of more than 95% w/w. Viscosity were in the range of 0.90 + 0.07 to 1.39 + 0.07 cps. The formulations showed newtonian flow characteristic that consistently exists between viscosity and shear rate as same as in CT/AA-SLN. The results of DSC and X-ray diffractions were corresponding similar that after AA was incorporated into SLN, the drug was not in crystalline state but in amorphous state. CT/AA-SLN displayed to enhance mucoadhesion property when compared with AA-SLN correlating to the concentration of added chitosan. The TEER values were not different after permeation experiments in all fomulations compared with initial. The % cell viability was more than 90% which correlated in cytotoxicity by MTT assay. TM TW80:SP85 5:5 was shown higher cellular uptake and permeation into human nasal epithelial carcinoma cell line than SOF TW80:SP85 5:5. CT/ AA-SLN that showed higher cellular uptake but lower permeation than AA-SLN which was agreement with in vitro permeation study thruogh 0.2 µm cellulose acetate membrane.
DOI: 10.14457/cu.the.2011.174
title: Anti-inflammatory activity of treehom remedy extract

description: Treehom remedy is a Thai traditional medicine used for relieving of pyrexia and constipation in children. Its composition are Terminalia sp., Terminalia bellirica Roxb., Phyllanthus emblica L., Coriandrum sativum Linn., Aristolochia sp., Angelica dahurica Benth., Glycyrrhiza glabra L., Sodium borate, Trigonella foenum-graecum L., Terminalia chebula Retz., Rheum officinale Baill. The aim of this study is to investigate the anti-inflammatory activity of the water and ethanol extracts from this remedy on LPS-activated J774A.1 macrophages. Our results demonstrated that the ethanol and water extracts of Treehom remedy significantly inhibited NO production from LPS-stimulated macrophages in concentration-dependent manner (6.25-100 µg/ml). IC₅₀ values were 40.05 and 60.05 µg/ml for the ethanol and water extracts respectively. Both extracts 25-100 µg/ml inhibited mRNA expression of inducible nitric oxide synthase (iNOS). The expressions were 71.28%- 29.58% and 73.97%-39.64% for the ethanol and water extracts, respectively and were correlated with the reduction in NO production. These extracts also reduced mRNA expression of cyclooxygenase 2 (COX-2) with the expression of 75.67%-38.32% and 86.81%-48.95% for the ethanol and water extracts. Moreover both extracts decreased mRNA expression of TNF-α, IL-1β, IL-6. These cytokines and COX-2 enzyme are inflammatory mediators produced during inflammatory process. The antioxidant assay also showed high antioxidant activity of both extracts at concentration 25-100 µg/ml. All of these result demonstrated anti-inflammatory potential of the ethanol and water extracts from Treehom remedy.
DOI: 10.14457/cu.the.2011.175
title: Development of LIPL32 DNA vaccine for leptospirosis using modified chitosan nanoparticles

description: Leptospirosis is a global zoonotic disease and public health problem in Thailand. In this study, we have developed a DNA vaccine by using mannosylated chitosan nanoplaticle-encapsulating plasmid carrying lipL32. The efficiency of the system was evaluated in vitro and in vivo. After transfection of plasmid containing full-length lipL32 gene (pVITRO-lipL32) into HEK293T cells, LipL32 was expressed in both the cell lysate and culture supernatant. The chitosan nanoparticles were modified by mannosylation to specifically target antigen presenting cells expressing mannose receptors, such as macrophages and dendritic cells. The chitosan and mannosylated chitosan nanoparticles were able to completely encapsulate the lipL32 DNA vaccine (CS-pVITRO-lipL32 and MC-pVITRO-lipL32, respectively). When RAW 264.7 cells were transfected with CS-pVITRO-lipL32 or MC-pVITRO-lipL32, no cytotoxicity was observed, and expression of LipL32 was detected. To evaluate immunogenicity, BALB/c mice were immunized with CS-pVITRO-lipL32 and MC-pVITRO-lipL32. Total IgG level of mice immunized with MC-pVITRO-lipL32 was significantly higher than that of CS-pVITRO-lipL32 and naked plasmid (pVITRO-lipL32) at 8 week of immunization (p&lt;0.05). In addition, mice immunized with MC-pVITRO-lipL32 vaccine tended to develop toward Th1 response but at the lower extent than that of chitosan-conjugated vaccine. Our results suggest that CS and MC nanoparticles are efficient delivery systems for lipL32 DNA vaccine to specifically induce humoral and Th1-predilected cell-mediated immune responses.
DOI: 10.14457/cu.the.2011.176
title: Plantwide control structures design for butyl acetate process

description: A structure of most industrial processes, including many different unit operations, several recycle streams and energy integration, is a complex flowsheet. Therefore plantwide control structure design is an important way to control processes which become more complex so that processes are designed and operated closer to constraints. This research is design of plantwide control structures for butyl acetate process, which consist of a reactor, three distillation columns and two recycle streams. It can be produced by the reaction of methyl acetate with butanol in a reversible, liquid-phase, mildly exothermic reaction. Methanol is the second product. The objective of this research is selection of controlled variables so that finding which variables that should be controlled system for quickly responding to disturbances and adjusting self to steady state while minimizing the deviation of the product quality. Plantwide control procedure of Wongsri(2012) is chosen for design the new control structures in order to develop control loops to achieve the required objectives. Steady state designs were performed in commercial process simulator and evaluate the performance using integrated absolute error (IAE) of the designed control structures.
DOI: 10.14457/cu.the.2011.177
title: Distribution centre selection model in Europe for a ceramic tile company

description: This thesis reports the details of the distribution centre location selection model for ceramic product new market in Germany in order to help reducing the alternatives and focusing on the plausible choices. It contains three process of model development (1) collect the requirements and constrains, (2) qualitative comparative analysis and (3) quantitative comparative analysis. Using the company criteria and applying relevant theories, the model is designed into systematical steps. While develop the model, the case study of Thai Ceramic Company is applied to illustrate the process of selection model that is useful and practical. The result from the model helps to make a decision and gives business advantages to the company.
DOI: 10.5517/ccdc.csd.cc1nq81p
title: CCDC 1540732: Experimental Crystal Structure Determination

description: Related Article: L. Singla, H.R. Yadav, A. Roy Choudhury|2020|Acta Crystallogr.,Sect.B:Struct.Sci.,Cryst.Eng. and Mat.|76|604|doi:10.1107/S2052520620006873
DOI: 10.5517/ccdc.csd.cc1nq82q
title: CCDC 1540733: Experimental Crystal Structure Determination

description: Related Article: L. Singla, H.R. Yadav, A. Roy Choudhury|2020|Acta Crystallogr.,Sect.B:Struct.Sci.,Cryst.Eng. and Mat.|76|604|doi:10.1107/S2052520620006873
DOI: 10.14457/cu.the.2011.178
title: Oseltamivir efficiency toward neuraminidase of mutant strains of influenza b virus using molecular dynamics simulations

description: The influenza B virus causes epidemics of influenza in every 2-3 years serving high morbidity in humans. Oseltamivir, the neuraminidase (NA) inhibitor blocking the releasing of new viruses, is an effective agent against influenza B virus, however, the oseltamivir resistances conferring the E119G, R152K and D198N substitutions have been reported. In this study, molecular dynamics (MD) simulation was performed on the wild-type and the three NA mutants with oseltamivir bound. Based on three different of MD simulations, the wild-type system is the most stability which oseltamivir strongly binds through the five key residues E119, D151, R152, R292 and R371. The notable loss of hydrogen bond and the decrease in per-residue decomposition energy at the mutated residues G119 and K152 compared to those of the wild-type were found to be the primary source of high-level oseltamivir resistance in the E119G and R152K mutants, respectively. In addition, the larger cavities in the NA active site conferring with these two mutations at the –NH3+ and –NHAc side chains, respectively, were compensated by more accessibility of water molecules. In contrast to the low level oseltamvir resistance in D198N mutant, this strain only showed slightly decreased in hydrogen bonds. According to the MM/PBSA binding free energies, the order of oseltamivir efficiency is of wild-type &gt; D198N &gt; E119G &gt; R152K in good agreement with the experimental IC50 values.
DOI: 10.14457/cu.the.2011.179
title: Comparison of line-1 methylation between smokers and non-smokers

description: Objective This study aimed to investigate the possibility that smoking may promote cancer development via LINE-1 hypomethylation. Material and methods The LINE-1 methylation in clinically normal oral mucosa of current smokers was compared to that of non-smokers by using combined bisulphite restriction analysis. Each LINE-1 sequence was categorised into 4 patterns depending on the methylation status and location of 2 CpG dinucleotides from 5’ to 3’; which included mCmC, uCuC, mCuC and uCmC. Of these, mC and uC represent methylated and unmethylated CpG, respectively. Results Despite there was no significant difference in the overall LINE-1 methylation level, the percentages of some methylation patterns were different. The %mCmC and %uCuC increased, while the %mCuC decreased in current smokers (p=0.002, 0.015 and &lt;0.0001, respectively). Additionally, the lower %mCuC still persisted in persons who had stopped smoking for over 1 year (p=0.001). The %mCuC also decreased in the higher pack-year smokers (p=0.028). Interestingly, the uCuC could rise from mCuC to uCmC, while mCmC could rise from mCuC only. We further analysed expression microarrays from the airway epithelia of smokers and found that smoking-associated intragenic LINE-1 sporadically repressed or activated host genes, compared to genes that do not contain LINE-1. Conclusion The smoking paradoxically increase or decrease LINE1 methylation of certain loci. Hypomethylated LINE-1 loci induced by smoking led to the same consequences as those associated with cancer.