DOI: 10.5061/dryad.6n9h6
title: Data from: Molecular phylogeny and morphological analysis of Tetraglochin (Rosaceae: Rosoideae: Sanguisorbeae) and recognition of the new species T. andina

description: Tetraglochin is distributed along the Andes from Peru to southern
 Argentina and central Chile. In order to test its monophyly and analyze
 relationships among species of this genus and allied genera, we conducted
 morphological studies and phylogenetic analyses using both nuclear (ITS)
 and cpDNA (trnL-F) sequences. As a result, Tetraglochin is supported as
 monophyletic, with the genus Margyricarpus as its sister group.
 Tetraglochin acanthocarpa and T. caespitosa are sister to the rest of the
 genus, which is grouped in two clades. One clade grows primarily
 distributed along the Central Andes, includes T. cristata, T. inermis,
 plus some specimens of T. ameghinoi, and has a coriaceous or papery and
 non translucent hypanthium. The other clade is mainly in the Southern
 Andes, contains T. alata and some specimens of T. ameghinoi, and has a
 membranous translucent hypanthium. Based on results obtained from
 morphological studies, molecular phylogenies, and species delimitation
 analyses, a new species T. andina , is proposed, segregated from T.
 ameghinoi. A key to identify all species herein recognized, and a map
 containing geographical distribution of them are provided, together with a
 description and illustration of the new species.
description: 1-Combined trnL-ITS data
 matrixCombined trnL-ITS data
 matrix for bayesian phylogenetic analyses1-Tetraglochin-ITS&trnLF.nex2-Tetraglochin-trnLF matrixTetraglochin-trnLF matrix alignment2-Tetraglochin-trnLF.fst3-Tetraglochin-ITS matrixTetraglochin-ITS matrix3-Tetraglochin-ITS.fst4-Tetraglochin-ITS&trnLF-50% MAJORITY RULE CONSENSUS TREE4-Tetraglochin-ITS&trnLF-50% MAJORITY RULE CONSENSUS TREE from Bayesian analysis with MrBayes 3.24-Tetraglochin-ITS&trnLF.treFIG. S2-Tetraglochin-trnL-F-Bayesian majority rule consensus treeFIG. S2. Bayesian 50% majority rule consensus tree obtained from 15002 trees generated by Bayesian inference with MrBayes in the phylogenetic analysis of Tetraglochin species using the trnL-F dataset. The values above/ below branches correspond to the Bayesian posterior probability (PP)/ parsimony bootstrap (PB). Only PP values &gt; 0.50 and PB &gt; 75% are showed. The specimens with voucher collection number were sequenced in our work. Scale bar = 0.02 substitutions per site.5-Tetraglochin-MCCT-trnL-F.jpgFIG. S1-Tetraglochin-MCCT-ITSFIG. S1. Bayesian Maximum Clade Credibility Tree obtained from 15002 trees generated by Bayesian inference with MrBayes in the phylogenetic analysis of Tetraglochin species using the ITS dataset. The values above/ below branches correspond to the Bayesian posterior probability (PP)/ parsimony bootstrap (PB). Only PP values &gt; 0.50 and PB &gt; 75% are showed. The specimens with voucher collection number were sequenced in our work. Scale bar = 0.06 substitutions per site.6-Tetraglochin-MCCT-ITS.jpg7-StarBEASTLog(tetrag-ITS&trnL-19taxa_1)xml file for species tree analyses using BEAST under the hypothesis that all individuals of Tetraglochin ameghinoi represent the same species8-StarBEASTLog(tetrag-ITS&trnL-19taxa_2)xml for species tree analyses using BEAST under the hypothesis that Tetraglochin ameghinoi specimens represent two different species, according to the clades obtained in the phylogenetic analyses and the morphological differentiation exhibited.