cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21793994.v1
title: Fig2G_RACE_STOML2_R1_LG361_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794039.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R2_LG297.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793373
title: Fig2B_RACE_STOML2_R2_LG292.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793373.v1
title: Fig2B_RACE_STOML2_R2_LG292.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793376
title: Fig2B_RACE_STOML2_R2_LG292.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793754
title: Fig2F_STOML2_ExtFig4C_BCAP31_RACE_R3_LG300.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793754.v1
title: Fig2F_STOML2_ExtFig4C_BCAP31_RACE_R3_LG300.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794039
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R2_LG297.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793376.v1
title: Fig2B_RACE_STOML2_R2_LG292.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793382
title: Fig2B_RACE_STOML2_R3_LG293_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793382.v1
title: Fig2B_RACE_STOML2_R3_LG293_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793391
title: Fig2B_RACE_STOML2_R3_LG293.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793391.v1
title: Fig2B_RACE_STOML2_R3_LG293.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793394
title: Fig2B_RACE_STOML2_R3_LG293.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793394.v1
title: Fig2B_RACE_STOML2_R3_LG293.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793397
title: Fig2B_RACE_STOML2_R2_LG292_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793397.v1
title: Fig2B_RACE_STOML2_R2_LG292_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793403
title: Fig2B_RACE_YKT6_R2_LG292.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793403.v1
title: Fig2B_RACE_YKT6_R2_LG292.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793400
title: Fig2B_YKT6_ExtFig3A_TUBA1B_RACE_R1_LG290.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793400.v1
title: Fig2B_YKT6_ExtFig3A_TUBA1B_RACE_R1_LG290.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793406
title: Fig2B_RACE_YKT6_R3_LG293.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793406.v1
title: Fig2B_RACE_YKT6_R3_LG293.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793415
title: Fig2B_RACE_YKT6_R3_LG293_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793415.v1
title: Fig2B_RACE_YKT6_R3_LG293_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793433
title: Fig2B_RACE_YKT6_R2_LG292_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793433.v1
title: Fig2B_RACE_YKT6_R2_LG292_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793442.v1
title: Fig2B_STOML2_ExtFig3A_BCAP31_RACE_R1_LG290.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793448
title: Fig2B_RACE_YKT6_R2_LG292.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793448.v1
title: Fig2B_RACE_YKT6_R2_LG292.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793442
title: Fig2B_STOML2_ExtFig3A_BCAP31_RACE_R1_LG290.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793451
title: Fig2E_18S-gel_R1-R3_LG379-374.sgd

description: <em>Xrn1 knock out A549 cells were infected with WT or PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.</em> <em>HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without the STOML2 99bp sequence insertion. RNA was extracted and analyzed by Northern blotting. Ethidium bromide pictures of gel showing rRNA are included.</em>
DOI: 10.6084/m9.figshare.21793451.v1
title: Fig2E_18S-gel_R1-R3_LG379-374.sgd

description: <em>Xrn1 knock out A549 cells were infected with WT or PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.</em> <em>HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without the STOML2 99bp sequence insertion. RNA was extracted and analyzed by Northern blotting. Ethidium bromide pictures of gel showing rRNA are included.</em>
DOI: 10.6084/m9.figshare.21793457
title: Fig2B_RACE_YKT6_R3_LG293.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793460
title: Fig2B_YKT6_ExtFig3A_TUBA1B_RACE_R1_LG290.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793460.v1
title: Fig2B_YKT6_ExtFig3A_TUBA1B_RACE_R1_LG290.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793472
title: Fig2B_STOML2_ExtFig3A_BCAP31_RACE_R1_LG290_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793472.v1
title: Fig2B_STOML2_ExtFig3A_BCAP31_RACE_R1_LG290_annotated.tiff

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793331
title: Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R3_LG262.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793457.v1
title: Fig2B_RACE_YKT6_R3_LG293.sgd

description: Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21793499
title: Fig2E_NB_luciferase_R1_LG374.tif

description: <em>Xrn1 knock out A549 cells were infected with WT or PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.</em> <em>HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without the STOML2 99bp sequence insertion. RNA was extracted and analyzed by Northern blotting. Ethidium bromide pictures of gel showing rRNA are included.</em>
DOI: 10.6084/m9.figshare.21793499.v1
title: Fig2E_NB_luciferase_R1_LG374.tif

description: <em>Xrn1 knock out A549 cells were infected with WT or PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.</em> <em>HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without the STOML2 99bp sequence insertion. RNA was extracted and analyzed by Northern blotting. Ethidium bromide pictures of gel showing rRNA are included.</em>
DOI: 10.6084/m9.figshare.21793307
title: Fig1B_qPCR_G6PD_WT-A549_R3_LG400.xlsx

description: A549 or A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793322
title: Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R2_LG261.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793322.v1
title: Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R2_LG261.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793331.v1
title: Fig1B-C_qPCR_G6PD_HA_Xrn1ko_R3_LG262.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels
DOI: 10.6084/m9.figshare.21793475
title: Fig2F_STOML2_ExtFig4C_BCAP31_RACE_R1_LG295.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793283
title: Fig1B_qPCR_G6PD_WT-A549_R1_ExtFig3B_HA_Xrn1ko_R2_LG292.xlsx

description: A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels