cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.vq73as
title: Occurrence Download

description: A dataset containing 1129 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Brassica rapa",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1129 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0041229-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.82qbvk
title: Occurrence Download

description: A dataset containing 105 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Styela clava",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 105 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0041230-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fhejb7
title: Occurrence Download

description: A dataset containing 9215330 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-82.44675 -13.00037,-49.69226 -13.00037,-49.69226 15.77775,-82.44675 15.77775,-82.44675 -13.00037))",
 "HasCoordinate is true",
 "TaxonKey is Plantae"
 ]
}

 The dataset includes 9215330 records from 2030 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0041119-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112048134
title: Cuneiform Tablet 1913.14.0225, Spurlock 1913.14.0225

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0225; CDLI P420607 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish Gray (10YR 8/2) Medium Gray (N 5.25/ 22.1% R) ❧ [Physical Object Note:] 3.1 x 3.1 x 1.9 cm (22g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.15468/dl.ndpwse
title: Occurrence Download

description: A dataset containing 151 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Alchemilla woodii Kuntze"
 ]
}

 The dataset includes 151 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009403-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.phngns
title: Occurrence Download

description: A dataset containing 10923 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-77.06097 6.78501,-83.25683 -6.35222,-77.83545 -13.9696,-70.61523 -19.72534,-74.73752 -38.66969,-75.51201 -49.22163,-75.77936 -52.84662,-68.54167 -55.80433,-56.44426 -36.97521,-39.4405 -20.55507,-38.00384 -13.93202,-33.98815 -6.12895,-50.81571 2.5327,-56.4753 8.15082,-56.4753 8.15082,-65.09575 13.38332,-76.43151 11.63422,-77.06097 6.78501))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Tayassu pecari (Link, 1795)"
 ]
}

 The dataset includes 10923 records from 75 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0411641-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112048139
title: Cuneiform Tablet 1913.14.0225, Spurlock 1913.14.0225

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0225; CDLI P420607 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish Gray (10YR 8/2) Medium Gray (N 5.25/ 22.1% R) ❧ [Physical Object Note:] 3.1 x 3.1 x 1.9 cm (22g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.15468/dl.h5mju5
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ranunculus weddellii Lourteig"
 ]
}

 The dataset includes 6 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0100660-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.86fkp2
title: Occurrence Download

description: A dataset containing 7031 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "Continent is Europe",
 "Year is greater than 1978",
 "Year is less than 2014",
 "HasGeospatialIssue is false",
 "TaxonKey is Wolffia arrhiza (L.) Horkel ex Wimm."
 ]
}

 The dataset includes 7031 records from 90 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0023045-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.hkzw5s
title: Occurrence Download

description: A dataset containing 310 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Asio abyssinicus (Guérin-Méneville, 1843)"
 ]
}

 The dataset includes 310 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0397681-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112048220
title: Cuneiform Tablet 1913.14.0225, Spurlock 1913.14.0225

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0225; CDLI P420607 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish Gray (10YR 8/2) Medium Gray (N 5.25/ 22.1% R) ❧ [Physical Object Note:] 3.1 x 3.1 x 1.9 cm (22g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.15468/dl.63cx7y
title: Occurrence Download

description: A dataset containing 751 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Penelopides exarhatus (Temminck, 1823)"
 ]
}

 The dataset includes 751 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0400317-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112048272
title: Cuneiform Tablet 1913.14.0225, Spurlock 1913.14.0225

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0225; CDLI P420607 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish Gray (10YR 8/2) Medium Gray (N 5.25/ 22.1% R) ❧ [Physical Object Note:] 3.1 x 3.1 x 1.9 cm (22g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.15468/dl.p3uy37
title: Occurrence Download

description: A dataset containing 71 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Serjania rufa Radlk."
 ]
}

 The dataset includes 71 records from 20 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0126060-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2curae
title: Occurrence Download

description: A dataset containing 8615 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is South America",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Tremarctos ornatus (F.G.Cuvier, 1825)"
 ]
}

 The dataset includes 8615 records from 67 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0129242-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112019873
title: Cuneiform Tablet 1913.14.0257, Spurlock 1913.14.0257

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0257; CDLI P420639 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Moderate Yellowish Pink (5YR 7/4) ❧ [Physical Object Note:] 4.5 x 4.3 x 1.5 cm (66g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.15468/dl.xvh9jr
title: Occurrence Download

description: A dataset containing 1054127 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is iNaturalist Research-grade Observations",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Country is one of (Canada, Mexico, United States of America)",
 "EventDate is greater than or equal to 2021-04-13",
 "EventDate is less than or equal to 2021-05-13",
 {
 "not" : {
 "Issue" : [
 "is one of (Basis of record invalid, Coordinate reprojection failed, Coordinate reprojection suspicious, Coordinate precision invalid, Coordinate uncertainty meters invalid, Country mismatch, Country invalid, Geodetic datum invalid, Presumed swapped coordinate, Presumed negated longitude, Presumed negated latitude, Country coordinate mismatch)"
 ]
 }
 }
 ]
}

 The dataset includes 1054127 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0397679-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c5pynx
title: Occurrence Download

description: A dataset containing 3934 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is United States of America",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Metasequoia glyptostroboides H.H.Hu & W.C.Cheng",
 "Year 1993-2023"
 ]
}

 The dataset includes 3934 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0235250-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.um8n7b
title: Occurrence Download

description: A dataset containing 100 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Serjania sphaerococca Radlk."
 ]
}

 The dataset includes 100 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0126077-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112050575
title: P.Oxy.VII 1030 Notification of Death, P.Oxy.VII 1030

description: [Text Description:] Notice of the death of a slave. ❧ [Text or Publication Number Note:] P.Oxy.VII 1030, P.Oxy. 1030, Spurlock 1914.21.0017 ❧ [Excavation Description:] Excavated by archaeologists, including Bernard Pyne Grenfell and Arthur Surridge Hunt, at an ancient rubbish dump near Oxyrhynchus in Egypt (28°32'N 30°40'E, modern el-Bahnasa). ❧ [Medium:] Papyrus ❧ [Script Note:] Greek ❧ [Physical Object Description:] Papyrus manuscript, 26.2 x 9.6 cm.
DOI: 10.15468/dl.959wad
title: Occurrence Download

description: A dataset containing 1282 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Trachylepis maculilabris (Gray, 1845), Trachylepis acutilabris (Peters, 1862))",
 "BasisOfRecord is one of (Specimen, Human Observation, Observation, Machine Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 1282 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0023064-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112050581
title: P.Oxy.VII 1030 Notification of Death, P.Oxy.VII 1030

description: [Text Description:] Notice of the death of a slave. ❧ [Text or Publication Number Note:] P.Oxy.VII 1030, P.Oxy. 1030, Spurlock 1914.21.0017 ❧ [Excavation Description:] Excavated by archaeologists, including Bernard Pyne Grenfell and Arthur Surridge Hunt, at an ancient rubbish dump near Oxyrhynchus in Egypt (28°32'N 30°40'E, modern el-Bahnasa). ❧ [Medium:] Papyrus ❧ [Script Note:] Greek ❧ [Physical Object Description:] Papyrus manuscript, 26.2 x 9.6 cm.
DOI: 10.15468/dl.s2n9mk
title: Occurrence Download

description: A dataset containing 19508 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Youngia japonica (L.) DC."
 ]
}

 The dataset includes 19508 records from 374 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0232332-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3yrtzr
title: Occurrence Download

description: A dataset containing 6496 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "Continent is Europe",
 "Year is greater than 1978",
 "Year is less than 2014",
 "HasGeospatialIssue is false",
 "TaxonKey is Melanitta perspicillata (Linnaeus, 1758)"
 ]
}

 The dataset includes 6496 records from 36 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019458-230224095556074/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112050528
title: P.Oxy.VIII 1074 Exodus 31,32, P.Oxy.VIII 1074

description: [Text Description:] Fragment of Septuagint Exodus, with verses 31:13-14, 32:7-8. ❧ [Text or Publication Number Note:] P.Oxy.VIII 1074, P.Oxy. 1074, Spurlock 1914.21.0021 ❧ [Excavation Description:] Excavated by archaeologists, including Bernard Pyne Grenfell and Arthur Surridge Hunt, at an ancient rubbish dump near Oxyrhynchus in Egypt (28°32'N 30°40'E, modern el-Bahnasa). ❧ [Medium:] Papyrus ❧ [Script Note:] Greek ❧ [Physical Object Description:] Papyrus manuscript, 2.7 x 5.1 cm.
DOI: 10.15468/dl.6gae7d
title: Occurrence Download

description: A dataset containing 7101787 species occurrences available in GBIF matching the query:
{
 "Country" : [
 "is China"
 ]
}

 The dataset includes 7101787 records from 4607 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0231834-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.h14r4z
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is (Table 1) Proportion of Toxoplasma gondii seropositive arctic foxes (Alopex lagopus) by age group and geographic area"
 ]
}

 The dataset includes 8 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0027600-180131172636756/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nrltpe
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is (Table 1) Proportion of Toxoplasma gondii seropositive arctic foxes (Alopex lagopus) by age group and geographic area"
 ]
}

 The dataset includes 8 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000023-160801135411260/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.1594/pangaea.949263
title: Pollen and non-pollen palynomorph counts above and below tephra layer V-18a, Lake Van
DOI: 10.14457/tu.the.2022.1591
title: ปัจจัยที่ส่งผลต่อการยอมรับการซื้อสินค้าออนไลน์ประเภทเครื่องประดับที่เป็นพลอยแท้

description: งานวิจัยในครั้งนี้เป็นงานวิจัยเชิงปริมาณมีวัตถุประสงค์เพื่อศึกษาปัจจัยที่ส่งผลต่อการยอมรับการซื้อสินค้าออนไลน์ประเภทเครื่องประดับที่เป็นพลอยแท้และเสนอแนะแนวทางในการพัฒนาการขายสินค้าออนไลน์ประเภทเครื่องประดับที่เป็นพลอยแท้ โดยใช้การประยุกต์จากแบบจำลองการยอมรับเทคโนโลยี แบบจำลองความสำเร็จของระบบสารสนเทศ และทฤษฎีส่วนประสมทางการตลาด (Marketing Mix) โดยมีการทดสอบความเที่ยงตรงเชิงเนื้อหา และความน่าเชื่อถือของแบบสอบถาม วิเคราะห์ข้อมูลด้วยสถิติเชิงอนุมาน ด้วยเทคนิคการวิเคราะห์องค์ประกอบเชิงสำรวจ (EFA) และวิเคราะห์ด้วยแบบจำลองสมการโครงสร้าง (SEM) จากการเก็บข้อมูลของกลุ่มตัวอย่างที่สุ่มด้วยวิธีแบบเฉพาะเจาะจงคือ ผู้ที่เคยซื้อเครื่องประดับพลอยแท้หรือเครื่องประดับมูลค่าสูงผ่านทางช่องทางออนไลน์ จำนวน 440 ตัวอย่าง ด้วยการใช้แบบสอบถามออนไลน์ ผลการวิจัย พบว่าปัจจัยที่ส่งผลต่อการยอมรับการซื้อสินค้าออนไลน์ประเภทเครื่องประดับที่เป็นพลอยแท้ประกอบด้วย 5 ปัจจัย ได้แก่ ปัจจัยด้านส่วนประสมการตลาดและผู้ขาย ปัจจัยด้านแพลตฟอร์มออนไลน์ ปัจจัยด้านผู้ซื้อ เป็นปัจจัยทางอ้อมที่ส่งผลผ่าน ปัจจัยด้านการรับรู้การใช้งานง่าย ปัจจัยการรับรู้ประโยชน์ และ ปัจจัยด้านการรับรู้ความเสี่ยง ที่เป็นปัจจัยทางตรงที่ส่งผลต่อการยอมรับการซื้อได้ที่ร้อยละ 32
DOI: 10.1594/pangaea.949265
title: Pollen and non-pollen palynomorph counts above and below tephra layer V-60, Lake Van
DOI: 10.1594/pangaea.949267
title: Pollen and non-pollen palynomorph counts above and below tephra layer V-176, Lake Van
DOI: 10.1594/pangaea.949268
title: Pollen and non-pollen palynomorph counts above and below tephra layer V-233a, Lake Van
DOI: 10.1594/pangaea.949269
title: Pollen and non-pollen palynomorph counts above and below tephra layer V-237, Lake Van
DOI: 10.14457/tu.the.2022.1592
title: การศึกษาผลประเมินความสามารถด้านดิจิทัลขององค์กรเพื่อเป็นแนวทางในการพัฒนาสมรรถนะการทำงานของบุคลากรส่วนงานบริการลูกค้าในระหว่างการเปลี่ยนแปลงด้านดิจิทัลในองค์กร: กรณีศึกษา บริษัทเอกชนผู้ผลิตวัสดุพอลิเมอร์แห่งหนึ่ง

description: การค้นคว้าอิสระนี้เป็นการทางานวิจัยศึกษาความพร้อมด้านดิจิทัลขององค์กรแห่งหนึ่งในช่วงระหว่างกระบวนการเปลี่ยนแปลงองค์กรด้วยดิจิทัลเพื่อค้นหาทักษะดิจิทัลที่จาเป็นต่อส่วนงานบริการลูกค้า เพื่อสามารถนาผลการศึกษาและข้อเสนอแนะที่ได้มาพัฒนา ปรับปรุงประสิทธิภาพคุณภาพการทางานบริการและเป็นแนวทางให้ภายในองค์กรได้ขับเคลื่อนไปในทิศทางที่เกิดผลสาเร็จได้โดยใช้วิธีการศึกษาแบบเชิงคุณภาพ (Qualitative Research) โดยศึกษาความสามารถด้านดิจิทัลขององค์กรและศึกษาจากข้อคิดเห็นของผู้บริหารถึงปัจจัยที่มีผลต่อทักษะด้านดิจิทัล ทั้ง 3 ด้าน ได้แก่(1) กลยุทธ์ดิจิทัล (2) ความพร้อมของเทคโนโลยี (3) โครงสร้างและวัฒนธรรมองค์กรผลการศึกษาความพร้อมและความสามารถด้านดิจิทัลขององค์กรตามปัจจัยข้างต้นประกอบกับข้อคิดเห็นของผู้บริหารเห็นว่าบุคลากรในสายงานบริการลูกค้าจาเป็นจะต้องมีทักษะการวิเคราะห์ขั้นสูง ทักษะการจัดการและแก้ปัญหาธุรกิจอิเล็กทรอนิกส์ และทักษะพื้นฐานในการจัดการด้านดิจิทัลพบว่าองค์กรที่ทาการศึกษามีช่องว่างและจุดอ่อนในการพัฒนาทักษะบุคลากรให้ตรงตามความคาดหวังที่วัดจากแบบประเมินซึ่งเกี่ยวกับข้องกับเป้าหมายการเปลี่ยนแปลงองค์กรด้วยดิจิทัล
DOI: 10.15468/dl.tvpxtj
title: Occurrence Download

description: A dataset containing 517652774 species occurrences available in GBIF matching the query:
(Query is too complex. Can be viewed on the landing page)

 The dataset includes 517652774 records from 10470 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002247-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.31044424
title: Raw flow cytometry dataset on E. coli plasmid hosts.

description: Plasmids are widely used across molecular biology and biotechnology, but robust replication is limited by stability issues in host cells. Here we systematically characterized transcriptional and physiological responses to plasmid burden in <i>Escherichia coli</i> across 3–586 copies per cell, multiple plasmid sizes, selection markers, in both glucose and glycerol. Using a machine-learning algorithm to extract co-regulated gene modules from transcriptomics data, we identified the SOS stress response as a signature of plasmid burden. The activation of the SOS response correlated strongly with the number of plasmid copies rather than total additional content of DNA, and resulted in cell filamentation through upregulation of the division inhibitor <i>sulA</i>. Using flow cytometry to measure cell size and complexity, we show that cell filamentation and cell size heterogeneity correlates with activation of the SOS response. These results demonstrate that plasmid instability arises from cellular stress driven by replication frequency rather than metabolic burden, and point to the SOS response as a key target for improving plasmid stability.
DOI: 10.6084/m9.figshare.31044424.v2
title: Raw flow cytometry dataset on E. coli plasmid hosts.

description: Plasmids are widely used across molecular biology and biotechnology, but robust replication is limited by stability issues in host cells. Here we systematically characterized transcriptional and physiological responses to plasmid burden in <i>Escherichia coli</i> across 3–586 copies per cell, multiple plasmid sizes, selection markers, in both glucose and glycerol. Using a machine-learning algorithm to extract co-regulated gene modules from transcriptomics data, we identified the SOS stress response as a signature of plasmid burden. The activation of the SOS response correlated strongly with the number of plasmid copies rather than total additional content of DNA, and resulted in cell filamentation through upregulation of the division inhibitor <i>sulA</i>. Using flow cytometry to measure cell size and complexity, we show that cell filamentation and cell size heterogeneity correlates with activation of the SOS response. These results demonstrate that plasmid instability arises from cellular stress driven by replication frequency rather than metabolic burden, and point to the SOS response as a key target for improving plasmid stability.
DOI: 10.6084/m9.figshare.31044424.v1
title: Raw flow cytometry dataset on E. coli plasmid hosts. Data files (.csv) contains measurements for 10.000 events.

description: Plasmids are widely used across molecular biology and biotechnology, but robust replication is limited by stability issues in host cells. Here we systematically characterized transcriptional and physiological responses to plasmid burden in <i>Escherichia coli</i> across 3–586 copies per cell, multiple plasmid sizes, selection markers, in both glucose and glycerol. Using a machine-learning algorithm to extract co-regulated gene modules from transcriptomics data, we identified the SOS stress response as a signature of plasmid burden. The activation of the SOS response correlated strongly with the number of plasmid copies rather than total additional content of DNA, and resulted in cell filamentation through upregulation of the division inhibitor <i>sulA</i>. Using flow cytometry to measure cell size and complexity, we show that cell filamentation and cell size heterogeneity correlates with activation of the SOS response. These results demonstrate that plasmid instability arises from cellular stress driven by replication frequency rather than metabolic burden, and point to the SOS response as a key target for improving plasmid stability.
DOI: 10.15468/dl.666sfg
title: Occurrence Download

description: A dataset containing 39673 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((116.667 -0.9367197, 119.5458 -0.9367197, 119.5458 2.308565, 116.667 2.308565, 116.667 -0.9367197))"
 ]
}

 The dataset includes 39673 records from 199 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014046-250515123054153/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.