cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/k9x8a-4y174
title: Vibrio deltaCheA2

description: V. cholerae strain deleted for CheA2
description: <strong>Tilt Series Date:</strong> 2014-04-10</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Ikuro Kawagishi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C</p>
  aba2014-04-10-18_slicer3834.jpg: short polar array and flagellar motor in delta CHeA2 strain
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-04-10-18/rawdata/</p>VibrioDeltaA20016.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-04-10-18/rawdata/VibrioDeltaA20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioDeltaA20016_part121_14.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-04-10-18/3dimage_41262/VibrioDeltaA20016_part121_14.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/96tpd-gpq49
title: E.coli   "strain 4" I241E

description: E.coli chemotaxis mutant  "strain 4" I241EReceptors (tsr on chromosome) locked in a CheA activating state
description: <strong>Tilt Series Date:</strong> 2012-03-06</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain generated by John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown in TB (gentamycin) overnight at 30 degrees. Diluted in fresh buffer and grown to log phase. Then incubated with penicillin for one hour.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: -4 offset, 3.5 s blot time, no drain, 100 percent humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-06-11/rawdata/</p>strain4marchsix0011.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-06-11/rawdata/strain4marchsix0011.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain4marchsix0011_part128_1.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-06-11/3dimage_17492/strain4marchsix0011_part128_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qfb6k-d9m42
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-04-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-30/rawdata/</p>20140415_YWC092_20030.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-30/rawdata/20140415_YWC092_20030.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140415_YWC092_20030_part131_13.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-30/3dimage_41683/20140415_YWC092_20030_part131_13.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5235g-d7989
title: Myxococcus xanthus delta-pilQ

description: grid box YWC061
description: <strong>Tilt Series Date:</strong> 2013-12-30</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-92/rawdata/</p>20131230_Janelia_YWC061_10030.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-92/rawdata/20131230_Janelia_YWC061_10030.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131230_Janelia_YWC061_10030_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.fkz8qb
title: Occurrence Download

description: A dataset containing 330 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Astrostole scabra",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 330 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007344-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/rw04x-4pa23
title: Mycobacter smegmatis pneumonia

description: KP3 WT (S) M. smegmatis mc2_155
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> mc2_155</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF Tomo  gatan K2  Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul / Eric Brown Genentech, Inc. 1 DNA Way South San Francisco, CA 94080 (650) 225-6248 Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Saunton Media (S)</p>
<strong>Sample Preparation:</strong> 3ul + 10nm Au R2/2 quantifoils 4.5 offset 1 blot 2s 1s drainsingle tilt. constant angular increment, step: 1. tilt range: (-60, 60).        dosage: 180/A2. defocus: -8um. magnification: 26000</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-35/rawdata/</p>atlas10035.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-35/rawdata/atlas10035.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10035_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-35/3dimage_30628/atlas10035_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q0q4q-ksc46
title: Myxococcus xanthus LL061

description: mCherry-bacP.First loaded in Titan cartridge and imaged in cryoLM.Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 29100x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-15/rawdata/</p>LL061_UCLA_correlated_20130424_4.mrc,  Tilt Series (Pixel Size 1.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-15/rawdata/LL061_UCLA_correlated_20130424_4.mrc"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_4_full.rec,  Reconstruction (Pixel Size 2.0 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-15/3dimage_26288/LL061_UCLA_correlated_20130424_4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dafm6-khe77
title: Myxococcus xanthus delta-pilC

description: grid box YWC060
description: <strong>Tilt Series Date:</strong> 2013-12-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-5/rawdata/</p>20131226_Janelia_YWC060_10035.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-5/rawdata/20131226_Janelia_YWC060_10035.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131226_Janelia_YWC060_10035_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-5/3dimage_37069/20131226_Janelia_YWC060_10035_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wtehk-4pd42
title: Myxococcus xanthus delta-pilA

description: grid box YWC059
description: <strong>Tilt Series Date:</strong> 2013-12-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-14/rawdata/</p>20131228_Janelia_YWC059_10005.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-14/rawdata/20131228_Janelia_YWC059_10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131228_Janelia_YWC059_10005_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-14/3dimage_37176/20131228_Janelia_YWC059_10005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f2eq2-fdb51
title: Hyphomonas "AR37"

description: ParA-like protein, HNE0708, venus
description: <strong>Tilt Series Date:</strong> 2012-03-03</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in Marine Broth (MB) medium at 30 degrees. Expression of Venus labeled HNK0708 was induced by adding final 300 uM CuCl2 and incubated at 30 degrees for 24 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2012-03-03-3_slicer8656.jpg: REZ  .  Ribosome exclusion zone .  Hyphomonas neptunium
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-03-03-3/rawdata/</p>2012Mar03_AR37_24hr_00003.mrc,  Tilt Series (Pixel Size 0.78 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-03-03-3/rawdata/2012Mar03_AR37_24hr_00003.mrc"
        > <i class="download icon"></i> Download </a></p>

        2012Mar03_AR37_24hr_00003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-03-03-3/3dimage_17380/2012Mar03_AR37_24hr_00003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gbjzn-99f85
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-22/rawdata/</p>20131221_YWC063_10022.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-22/rawdata/20131221_YWC063_10022.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_10022_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-22/3dimage_36422/20131221_YWC063_10022_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.34740/kaggle/dsv/12908242
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.48472/deposita/mgasn9
title: Acessibilidade digital nos periódicos científicos vinculados ao Portal de Periódicos Udesc

description: Periódicos avaliados pelo accessMonitor e Wave em 09-04-2025 e em em 24-08-2025.
DOI: 10.22002/8ghcn-7j719
title: Hyphomonas AR46

description: ParA-like protein, HNE0708 knockout
description: <strong>Tilt Series Date:</strong> 2012-09-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown overnight in Marine Broth (MB) medium at 30 degrees without antibiotics. OD600=0.6</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-07-10/rawdata/</p>20120907_AR46_10010.mrc,  Tilt Series (Pixel Size 1.0 nm), 925.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-07-10/rawdata/20120907_AR46_10010.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120907_AR46_10010_full.rec,  Reconstruction (Pixel Size 2.0 nm), 262.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-07-10/3dimage_20682/20120907_AR46_10010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gx1ww-6r634
title: CheA-GE8-L2

description: silver binding peptide in linker 2 on CheAData collected on TITAN HHMI
description: <strong>Tilt Series Date:</strong> 2012-10-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: °, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> FEI Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Carly Carter, Dan Feldheim</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB, induced with 14 mM IPTG for 3 hours, lysed with penicillin for 1 hour, add DNAse buffer, DNAse for 20 min, add AgNO3 (0.014g AgNO3 in 1ml HEPES pH 7.2) and 50µl to 1 ml, incubated 20 min, spin down at 6krpm</p>
<strong>Sample Preparation:</strong> plunge freeze on Quantifoil R2/2 in liquid ethane/propane mix</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-17/rawdata/</p>CheA_AG-1_19_orig.st,  Tilt Series (Pixel Size 0.0 nm), 1.1 GB  
        
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        CheA_AG-1_19_full.rec,  Reconstruction (Pixel Size 0.0 nm), 309.8 MB  
        
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