DOI: 10.5061/dryad.h18931zvn
title: Associations of NPPA promoter true methylation and hydroxymethylation with ischemic stroke and its functional outcome

description: Background Atrial natriuretic peptide (ANP) has been associated with
 ischemic stroke (IS), but the underlying molecular mechanisms are not
 clear. The objective of this study was to examine whether DNA methylation
 and hydroxymethylation in the coding gene of ANP (NPPA) were associated
 with IS, as well as its functional outcome. Methods and Results DNA
 methylation and hydroxymethylation in NPPA promoter region were quantified
 by targeted bisulfite sequencing and APOBEC-coupled epigenetic sequencing,
 respectively, in 615 IS patients and 610 age- and sex-matched healthy
 controls. True methylation was calculated as the quantified methylation
 level minus the quantified hydroxymethylation level. The functional
 outcome of IS was evaluated by the modified Rankin Scale (mRS) at a
 3-month follow-up visit after onset. Multiple testing was controlled by
 the false discovery rate approach. Of the nine CpG sites assayed,
 hypomethylation at eight CpGs was not only associated with an increased
 risk of having IS but also predicted a higher mRS score after onset (all
 q&lt;0.05) and hydroxymethylation at one CpG located at Chr1:11908348
 was positively associated with IS (OR=1.39, 95%CI:1.15-1.68,
 q&lt;0.05), after adjusting for covariates and multiple testing.
 While, hydroxymethylation levels at none of the CpGs assayed were
 significantly associated with the mRS score. Conclusions DNA methylation
 and hydroxymethylation in NPPA promoter were associated with the risk of
 IS and its functional outcome in Chinese adults.
description: # Associations of NPPA Promoter True Methylation and Hydroxymethylation
 with Ischemic Stroke and Its Functional Outcome
 [https://doi.org/10.5061/dryad.h18931zvn](https://doi.org/10.5061/dryad.h18931zvn) ## Description of the data and file structure DNA methylation and hydroxymethylation in *NPPA* promoter region were quantified by targeted bisulfite sequencing and APOBEC-coupled epigenetic sequencing, respectively, in 615 IS patients and 610 age- and sex-matched healthy controls. True methylation was calculated as the quantified methylation level minus the quantified hydroxymethylation level. ### Files and variables #### File: NPPA\_hmc.csv **Description:**  Hydroxymethylation levels at 9 CpG sites in the promoter region of NPPA gene，the unit of Hydroxymethylation is %. ##### Variables * CpG site: genetic location of the CpG site * Chr: Chromsome * Distance2TSS: Distance to transcription site * GenomePosition: genome position * Position: functional position * Target: the target region of the sequencing * Type: The sequence of the CpG site is CG or not. C means cytosin. G means guanine. * X110001 - X750056: indicate the ID of participants #### File: NPPA\_mc.csv **Description:** Methylation levels at 9 CpG sites in the promoter region of NPPA gene, the unit of methylation is %. ##### Variables * CpG site: genetic location of the CpG site * Chr: Chromsome * Distance2TSS: Distance to transcription site * GenomePosition: genome position * Position: functional position * Target: the target region of the sequencing * Type: The sequence of the CpG site is CG or not. C means cytosin. G means guanine. * X110001 - X750056: indicate the ID of participants #### File: NPPA\_tmc.csv **Description:** True methylation levels at 9 CpG sites in the promoter region of NPPA gene, the unit of true methylation is %. ##### Variables * CpG site: genetic location of the CpG site * Chr: Chromsome * Distance2TSS: Distance to transcription site * GenomePosition: genome position * Position: functional position * Target: the target region of the sequencing * Type: The sequence of the CpG site is CG or not. C means cytosin. G means guanine. * X110001 - X750056: indicate the ID of participants ## Code/software The datasets are saved in CSV formate. Any softwares like SAS and R statistics could open these files.