cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19944656
title: Microbiome - Chalifour et al (2022) Century-Old Gut Microbiome Sequence Data

description: 16s rRNA gene amplicon sequence data to go with Chalifour, Elder, Li (2022) Microbiome manuscript.
DOI: 10.6084/m9.figshare.19944676
title: Genetic variability of Physalis ixocarpa and P. philadelphica from physicochemical fruit traits

description: Abstract The objective of this work was to estimate the genetic parameters and genetic divergence of two species of Physalis based on fruit traits, as well as the correlations between these traits. The experiment was carried out in a randomized complete block, with three treatments (P. philadelphica and the green and purple varieties of P. ixocarpa), and six replicates. The following traits were evaluated: average fruit mass (AFM), soluble solids (SS), longitudinal fruit diameter (LFD), transversal fruit diameter (TFD), total fruit mass (TFM), number of fruit per plant (NF), reducing sugars (RS), and total sugars (TS). Genetic divergence was estimated by Tocher’s and the UPGMA methods. There was genetic divergence between the Physalis species, with a higher contribution of AFM, LFD, and TFD, mainly by Tocher’s method. A positive correlation was observed between AFM x LFD, AFM x TFD, and LFD x TFD, and a negative correlations between LFD x TFM, TFD x TFM, and NF x RS. The species were discriminated by total sugars. The AFM, LFD, TFD, and TS traits show significant genetic variation and high values of heritability; therefore, they are suitable targets for the genetic breeding of the evaluated Physalis species.
DOI: 10.6084/m9.figshare.19944676.v1
title: Genetic variability of Physalis ixocarpa and P. philadelphica from physicochemical fruit traits

description: Abstract The objective of this work was to estimate the genetic parameters and genetic divergence of two species of Physalis based on fruit traits, as well as the correlations between these traits. The experiment was carried out in a randomized complete block, with three treatments (P. philadelphica and the green and purple varieties of P. ixocarpa), and six replicates. The following traits were evaluated: average fruit mass (AFM), soluble solids (SS), longitudinal fruit diameter (LFD), transversal fruit diameter (TFD), total fruit mass (TFM), number of fruit per plant (NF), reducing sugars (RS), and total sugars (TS). Genetic divergence was estimated by Tocher’s and the UPGMA methods. There was genetic divergence between the Physalis species, with a higher contribution of AFM, LFD, and TFD, mainly by Tocher’s method. A positive correlation was observed between AFM x LFD, AFM x TFD, and LFD x TFD, and a negative correlations between LFD x TFM, TFD x TFM, and NF x RS. The species were discriminated by total sugars. The AFM, LFD, TFD, and TS traits show significant genetic variation and high values of heritability; therefore, they are suitable targets for the genetic breeding of the evaluated Physalis species.
DOI: 10.6084/m9.figshare.19944677
title: Vegetative propagation of Campomanesia phaea by the air-layering and grafting techniques

description: Abstract: The objective of this work was to evaluate the air-layering and grafting techniques in the production of cambuci (Campomanesia phaea) plants. Two experiments were conducted in randomized complete blocks for air-layering, using adult cambuci plants, and two completely randomized for the grafting, using seedlings. Air layering was evaluated with different rates of indolebutyric acid; the splice, side-veneer, and chip-budding techniques were used for grafting. Air-layering rooting was not favored by the application of indolebutyric acid rates, whereas side-veneer grafts ensured the fixation of 6% of the grafts. Histological analyses of the air layers revealed the action of parenchyma cells from the xylem in the formation of structures similar to calluses. In the grafted plants, these cells ensured tissue union and regeneration. The air-layering technique was unfeasible, but grafting is recommended to establish cambuci tree genotypes.
DOI: 10.6084/m9.figshare.19944677.v1
title: Vegetative propagation of Campomanesia phaea by the air-layering and grafting techniques

description: Abstract: The objective of this work was to evaluate the air-layering and grafting techniques in the production of cambuci (Campomanesia phaea) plants. Two experiments were conducted in randomized complete blocks for air-layering, using adult cambuci plants, and two completely randomized for the grafting, using seedlings. Air layering was evaluated with different rates of indolebutyric acid; the splice, side-veneer, and chip-budding techniques were used for grafting. Air-layering rooting was not favored by the application of indolebutyric acid rates, whereas side-veneer grafts ensured the fixation of 6% of the grafts. Histological analyses of the air layers revealed the action of parenchyma cells from the xylem in the formation of structures similar to calluses. In the grafted plants, these cells ensured tissue union and regeneration. The air-layering technique was unfeasible, but grafting is recommended to establish cambuci tree genotypes.
DOI: 10.6084/m9.figshare.19944679.v1
title: Enzymatic association in the diets of laying hens raised in an alternative cage-free system

description: Abstract: The objective of this work was to evaluate the use of enzyme combinations in diets, with different nutritional uplift matrices, for hens raised in an alternative cage-free system. The experiment was carried out with 800 Isa Brown laying hens aged 24-30 weeks, distributed in a 2x2 factorial arrangement, with two combinations of enzymes (phytase and xylanase) and two nutritional matrices (conventional and overvalued uplifts). The treatments were: T1, phytase (450 FTU per kilogram) + xylanase (12,000 BXU per kilogram), using matrix I (100 Kcal kg-1 apparent metabolizable energy, 0.16% calcium, 0.15% available phosphorus, 0.03 sodium, and 0.02% digestible lysine); T2, phytase (1,500 FTU per kilogram) and matrix I; T3, phytase (450 FTU per kilogram) + xylanase (12,000 BXU per kilogram), using matrix II (120 Kcal kg-1 apparent metabolizable energy, 0.22% calcium, 0.20% available phosphorus, 0.04% sodium, and 0.05% digestible lysine); and T4, phytase (1,500 FTU per kilogram) and matrix II. Productive performance variables and external and internal egg quality were analyzed. The use of phytase or of the phytase + xylanase combination, independently of the nutritional matrix used, met the nutrient requirements of the animals and maintained their productive performance. However, the combination phytase + xylanase and the adoption of matrix I was more efficient.
DOI: 10.6084/m9.figshare.19944679
title: Enzymatic association in the diets of laying hens raised in an alternative cage-free system

description: Abstract: The objective of this work was to evaluate the use of enzyme combinations in diets, with different nutritional uplift matrices, for hens raised in an alternative cage-free system. The experiment was carried out with 800 Isa Brown laying hens aged 24-30 weeks, distributed in a 2x2 factorial arrangement, with two combinations of enzymes (phytase and xylanase) and two nutritional matrices (conventional and overvalued uplifts). The treatments were: T1, phytase (450 FTU per kilogram) + xylanase (12,000 BXU per kilogram), using matrix I (100 Kcal kg-1 apparent metabolizable energy, 0.16% calcium, 0.15% available phosphorus, 0.03 sodium, and 0.02% digestible lysine); T2, phytase (1,500 FTU per kilogram) and matrix I; T3, phytase (450 FTU per kilogram) + xylanase (12,000 BXU per kilogram), using matrix II (120 Kcal kg-1 apparent metabolizable energy, 0.22% calcium, 0.20% available phosphorus, 0.04% sodium, and 0.05% digestible lysine); and T4, phytase (1,500 FTU per kilogram) and matrix II. Productive performance variables and external and internal egg quality were analyzed. The use of phytase or of the phytase + xylanase combination, independently of the nutritional matrix used, met the nutrient requirements of the animals and maintained their productive performance. However, the combination phytase + xylanase and the adoption of matrix I was more efficient.
DOI: 10.6084/m9.figshare.19944682
title: Potassium application for sugarcane in a sandy loam Oxisol of the Brazilian Cerrado

description: Abstract: The objective of this work was to evaluate rates and methods of potassium application for plant cane (Saccharum spp.) grown in a dystrophic Typic Haplustox with a sandy loam texture in the Brazilian Cerrado. The experiment was carried out in the municipality of Aporé, in the state of Goiás, using the RB92579 sugarcane variety. The experimental design was randomized complete blocks, with three replicates, in a 5x3 factorial arrangement, with five potassium rates (0, 60, 110, 160, and 200 kg ha-1 K2O as potassium chloride) and three application methods (100% at the bottom of the planting furrow and covered with soil, 100% side-dressed on the sugarcane rows 60 days after planting, and 50% in the planting furrow and 50% side-dressed). The amount of potassium in the stalks in the control treatment was greater than that of the potassium stock extractable by Mehlich-1 in the 0-60 cm soil layer. Stalk and sugar yields increase linearly with potassium rates, approximately 20% with 200 kg ha-1 K2O compared with the control treatment (without potassium). Potassium content in stalks also increases with potassium rates. There is no significant effect of the potassium application methods on stalk and sugar yields and on cane quality variables.
DOI: 10.6084/m9.figshare.19944682.v1
title: Potassium application for sugarcane in a sandy loam Oxisol of the Brazilian Cerrado

description: Abstract: The objective of this work was to evaluate rates and methods of potassium application for plant cane (Saccharum spp.) grown in a dystrophic Typic Haplustox with a sandy loam texture in the Brazilian Cerrado. The experiment was carried out in the municipality of Aporé, in the state of Goiás, using the RB92579 sugarcane variety. The experimental design was randomized complete blocks, with three replicates, in a 5x3 factorial arrangement, with five potassium rates (0, 60, 110, 160, and 200 kg ha-1 K2O as potassium chloride) and three application methods (100% at the bottom of the planting furrow and covered with soil, 100% side-dressed on the sugarcane rows 60 days after planting, and 50% in the planting furrow and 50% side-dressed). The amount of potassium in the stalks in the control treatment was greater than that of the potassium stock extractable by Mehlich-1 in the 0-60 cm soil layer. Stalk and sugar yields increase linearly with potassium rates, approximately 20% with 200 kg ha-1 K2O compared with the control treatment (without potassium). Potassium content in stalks also increases with potassium rates. There is no significant effect of the potassium application methods on stalk and sugar yields and on cane quality variables.
DOI: 10.6084/m9.figshare.19944683
title: Selection of experimental strawberry clones for fruit appearance attributes

description: Abstract The objective of this work was to carry out the morphological characterization of strawberry fruit of different genotypes, as well as to verify their postharvest conservation. Six commercial cultivars (Albion, Aromas, Dover, Festival, Pircinque, and San Andreas) and nine experimental clones (MDA01, MDA19, MDA22, MDA23, MCA89, MCA94, MFA443, MOGSC468, and MFA443PR) were evaluated. Fruit were produced on semi-hydroponic gutters in protected cultivation and classified according to size, shape, color, brightness, achene position, pulp color, “heart” color, fruit cavity, and sepal position. For the postharvest analysis of fruit, the loss of mass and the appearance of fruit during ten days of storage were determined. The evaluated genotypes show a high similarity to each other regarding morphological characterization. MCA89 and 'Pircinque' are the most divergent genotypes in relation to the others. There is an association between the characteristics large cavity, reniform shape, and medium cavity, which contributes to the indirect selection of desirable characters. On the tenth day of storage, the MDA01, MDA19, MCA89, MFA443, and 'Dover' genotypes still show acceptable conditions for commercialization.
DOI: 10.6084/m9.figshare.19944683.v1
title: Selection of experimental strawberry clones for fruit appearance attributes

description: Abstract The objective of this work was to carry out the morphological characterization of strawberry fruit of different genotypes, as well as to verify their postharvest conservation. Six commercial cultivars (Albion, Aromas, Dover, Festival, Pircinque, and San Andreas) and nine experimental clones (MDA01, MDA19, MDA22, MDA23, MCA89, MCA94, MFA443, MOGSC468, and MFA443PR) were evaluated. Fruit were produced on semi-hydroponic gutters in protected cultivation and classified according to size, shape, color, brightness, achene position, pulp color, “heart” color, fruit cavity, and sepal position. For the postharvest analysis of fruit, the loss of mass and the appearance of fruit during ten days of storage were determined. The evaluated genotypes show a high similarity to each other regarding morphological characterization. MCA89 and 'Pircinque' are the most divergent genotypes in relation to the others. There is an association between the characteristics large cavity, reniform shape, and medium cavity, which contributes to the indirect selection of desirable characters. On the tenth day of storage, the MDA01, MDA19, MCA89, MFA443, and 'Dover' genotypes still show acceptable conditions for commercialization.
DOI: 10.6084/m9.figshare.19944685
title: Survival of Campylobacter jejuni in chicken at refrigeration and cooking temperatures

description: Abstract: The objective of this work was to evaluate the reduction of Campylobacter jejuni in chicken meat at 6 and 70°C, after the inoculation of a Brazilian strain. The kinetics of C. jejuni survival showed a 0.26 log (CFU g-1) decrease for each day of exposure at 6°C, and a 1.35 log (CFU g-1) decrease for each 1 log increase at 70°C. Although reduced levels of C. jejuni were found after regular intervals at both temperatures, its incomplete inactivation underlines the need of good hygiene practices for consumers to avoid campylobacteriosis.
DOI: 10.6084/m9.figshare.19944685.v1
title: Survival of Campylobacter jejuni in chicken at refrigeration and cooking temperatures

description: Abstract: The objective of this work was to evaluate the reduction of Campylobacter jejuni in chicken meat at 6 and 70°C, after the inoculation of a Brazilian strain. The kinetics of C. jejuni survival showed a 0.26 log (CFU g-1) decrease for each day of exposure at 6°C, and a 1.35 log (CFU g-1) decrease for each 1 log increase at 70°C. Although reduced levels of C. jejuni were found after regular intervals at both temperatures, its incomplete inactivation underlines the need of good hygiene practices for consumers to avoid campylobacteriosis.
DOI: 10.6084/m9.figshare.19944686
title: Functionality of cassava genotypes for waxy starch

description: Abstract: The objective of this work was to select cassava (Manihot esculenta) genotypes from the Brazilian germplasm bank with a functionality similar to that of waxy starch. A total of 881 genotypes were pre-selected using principal component analysis and hierarchical clustering, and their industrial potential was compared with that of the 7745-5WX waxy cassava and the WX-Maize waxy maize clones, both used as references. Two genotypes stood out: BGM0036 and BGM0083. Samples from these four genotypes were characterized by means of amylose content, scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, paste viscosity, and chain-length distribution of amylopectin. The samples presented A-type crystallinity and no statistical differences (p &gt; 0.05) regarding crystallinity degree (25.3 to 30.0), which shows similar proportions of the amylose/amylopectin fractions. No differences were observed in the microstructure of the cassava starches. Initial gelatinization temperature and amylopectin short chains presented a very strong negative correlation, indicating that a lower proportion of short chains of amylopectin results in a higher initial temperature of gelatinization. BGM0036 and BGM0083 show a low final viscosity, close to that of the waxy maize and cassava starches, being an alternative for use in different foods that require stability during freezing.
DOI: 10.6084/m9.figshare.19944686.v1
title: Functionality of cassava genotypes for waxy starch

description: Abstract: The objective of this work was to select cassava (Manihot esculenta) genotypes from the Brazilian germplasm bank with a functionality similar to that of waxy starch. A total of 881 genotypes were pre-selected using principal component analysis and hierarchical clustering, and their industrial potential was compared with that of the 7745-5WX waxy cassava and the WX-Maize waxy maize clones, both used as references. Two genotypes stood out: BGM0036 and BGM0083. Samples from these four genotypes were characterized by means of amylose content, scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, paste viscosity, and chain-length distribution of amylopectin. The samples presented A-type crystallinity and no statistical differences (p &gt; 0.05) regarding crystallinity degree (25.3 to 30.0), which shows similar proportions of the amylose/amylopectin fractions. No differences were observed in the microstructure of the cassava starches. Initial gelatinization temperature and amylopectin short chains presented a very strong negative correlation, indicating that a lower proportion of short chains of amylopectin results in a higher initial temperature of gelatinization. BGM0036 and BGM0083 show a low final viscosity, close to that of the waxy maize and cassava starches, being an alternative for use in different foods that require stability during freezing.
DOI: 10.6084/m9.figshare.19944688
title: β-lactam resistance in coagulase-negative Staphylococcus isolated from subclinical goat mastites

description: Abstract: The objective of this work was to evaluate resistance mechanisms to β-lactam antimicrobials in 251 strains of coagulase-negative Staphylococcus (CoNS) isolated from subclinical goat mastitis, as well as to determine the sensitivity and specificity of the nitrocefin and disk diffusion methods to detect penicillin resistance, in comparison with the polymerase chain reaction (PCR). The isolates were evaluated for the presence of the blaZ and mecA genes, β-lactamase production, and susceptibility to penicillin. Of the total isolates, 228 (91%) carried the blaZ gene and, among these, 144 (63%) were positive for β-lactamase production. Resistance to penicillin was observed in 125 of the isolates, of which 96.8% carried the blaZ gene. The sensitivity of the phenotypic methods to detect β-lactamase production was low, but their specificity was high; the Kappa coefficient showed a poor agreement between the phenotypic methods and PCR. The mecA gene was detected in only 3% of the isolates, which were identified as belonging to the species: S. capitis subsp. ureolyticus, S. caprae, S. warneri, S. sciuri, S. simulans, and S. cohnii subsp. urealyticum. Coagulase-negative Staphylococcus are important mastitis-causing pathogens in goat and harbor the blaZ and mecA genes related to resistance to β-lactam antimicrobials. The sensitivity of the nitrocefin and disk diffusion methods to detect penicillin resistance is low in relation to that of PCR.
DOI: 10.6084/m9.figshare.19944688.v1
title: β-lactam resistance in coagulase-negative Staphylococcus isolated from subclinical goat mastites

description: Abstract: The objective of this work was to evaluate resistance mechanisms to β-lactam antimicrobials in 251 strains of coagulase-negative Staphylococcus (CoNS) isolated from subclinical goat mastitis, as well as to determine the sensitivity and specificity of the nitrocefin and disk diffusion methods to detect penicillin resistance, in comparison with the polymerase chain reaction (PCR). The isolates were evaluated for the presence of the blaZ and mecA genes, β-lactamase production, and susceptibility to penicillin. Of the total isolates, 228 (91%) carried the blaZ gene and, among these, 144 (63%) were positive for β-lactamase production. Resistance to penicillin was observed in 125 of the isolates, of which 96.8% carried the blaZ gene. The sensitivity of the phenotypic methods to detect β-lactamase production was low, but their specificity was high; the Kappa coefficient showed a poor agreement between the phenotypic methods and PCR. The mecA gene was detected in only 3% of the isolates, which were identified as belonging to the species: S. capitis subsp. ureolyticus, S. caprae, S. warneri, S. sciuri, S. simulans, and S. cohnii subsp. urealyticum. Coagulase-negative Staphylococcus are important mastitis-causing pathogens in goat and harbor the blaZ and mecA genes related to resistance to β-lactam antimicrobials. The sensitivity of the nitrocefin and disk diffusion methods to detect penicillin resistance is low in relation to that of PCR.
DOI: 10.5281/zenodo.6600121
title: Sentinel 2 meltpond volume

description: Melt pond volumes based on the threshold-based method for lake detection/volume of Moussavi et al., 2020, to 19213 Sentinel-1 Level-1C top-of-atmosphere reflectance granules with less than 30% cloud cover and solar elevation angles above 25 degrees over all Antarctic ice shelves and the surrounding ice sheet. Each granule is processed in the Google Earth Engine at 100 m resampled resolution and converted into lake presence and lake depth. The depth observations were subsequently converted into total accumulated meltwater volumes per lake (in meter water equivalent) by integrating the derived lake depths per pixel over all granules. The resulting melt pond volume dataset (this repository) is then aggregated on the 27 km RACMO2.3p2 grid, accumulating the melt ponds that fall within one model grid cell. Original lake volumes per pixel are available through https://code.earthengine.google.com/?asset=projects/ee-earthmapps/assets/S2_LakesAntarctica_v3
description: {"references": ["Moussavi, M. S., Pope, A., Halberstadt, A. R. W., Trusel, L. D., Cioffi, L., and Abdalati, W. (2020). Antarctic supraglacial lake detection using Landsat 8 and Sentinel-2 imagery: towards continental generation of lake volumes. Remote Sensing, 12(134)."]}
DOI: 10.5281/zenodo.6600120
title: Sentinel 2 meltpond volume

description: Melt pond volumes based on the threshold-based method for lake detection/volume of Moussavi et al., 2020, to 19213 Sentinel-1 Level-1C top-of-atmosphere reflectance granules with less than 30% cloud cover and solar elevation angles above 25 degrees over all Antarctic ice shelves and the surrounding ice sheet. Each granule is processed in the Google Earth Engine at 100 m resampled resolution and converted into lake presence and lake depth. The depth observations were subsequently converted into total accumulated meltwater volumes per lake (in meter water equivalent) by integrating the derived lake depths per pixel over all granules. The resulting melt pond volume dataset (this repository) is then aggregated on the 27 km RACMO2.3p2 grid, accumulating the melt ponds that fall within one model grid cell. Original lake volumes per pixel are available through https://code.earthengine.google.com/?asset=projects/ee-earthmapps/assets/S2_LakesAntarctica_v3
description: {"references": ["Moussavi, M. S., Pope, A., Halberstadt, A. R. W., Trusel, L. D., Cioffi, L., and Abdalati, W. (2020). Antarctic supraglacial lake detection using Landsat 8 and Sentinel-2 imagery: towards continental generation of lake volumes. Remote Sensing, 12(134)."]}