cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21920430.v1
title: ExtFig9A_RACE_PR8-HA_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 ko A549 cells were infected with WT or PR8-PA(∆X), or mock infected. RNA was extracted to run 5’ RACE using primers ~150-200 nt downstream of GCUG sites that are predicted to be inside a hairpin loop within viral mRNAs (in segment 4 or 8 as indicated)
DOI: 10.6084/m9.figshare.21916728.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R2_LG323.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916731
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R1_LG322.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916731.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R1_LG322.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916734
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R1_LG322.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916734.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R1_LG322.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916737
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R2_LG323.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916737.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R2_LG323.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916740
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R3_LG324.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916740.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R3_LG324.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916743.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R3_LG324.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916746
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R3_LG324_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916698
title: ExtFig7C_qPCR_STOML2-YKT6-mut-int_R1_LG322.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21916698.v1
title: ExtFig7C_qPCR_STOML2-YKT6-mut-int_R1_LG322.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21916743
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R3_LG324.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21916746.v1
title: ExtFig7E_RACE_STOML2-YKT6-mut-int_R3_LG324_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with a mutation in the original luciferase cut sites and insertions in the introns (either from the STOML2 or YKT6 gene), with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE.
DOI: 10.6084/m9.figshare.21815946
title: ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21916701
title: ExtFig7C_qPCR_STOML2-YKT6-int_R1_LG314.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21916701.v1
title: ExtFig7C_qPCR_STOML2-YKT6-int_R1_LG314.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21916704.v1
title: ExtFig7C_qPCR_STOML2-YKT6-mut-int_R2-R3_LG323-324.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21916707
title: ExtFig7C_qPCR_STOML2-YKT6-int_R2-R3_LG316-318.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21815943
title: ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815943.v1
title: ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815946.v1
title: ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815949
title: ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21916704
title: ExtFig7C_qPCR_STOML2-YKT6-mut-int_R2-R3_LG323-324.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21916707.v1
title: ExtFig7C_qPCR_STOML2-YKT6-int_R2-R3_LG316-318.xlsx

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR
DOI: 10.6084/m9.figshare.21810291.v1
title: ExtFig6B_RACE_STOML2_R1_LG388_annotated.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21815949.v1
title: ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815952
title: ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815952.v1
title: ExtFig7B_BCAP31_intron_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815955.v1
title: ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21810291
title: ExtFig6B_RACE_STOML2_R1_LG388_annotated.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810294.v1
title: ExtFig6B_RACE_STOML2_R1_LG388_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21815955
title: ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21815958
title: ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21810294
title: ExtFig6B_RACE_STOML2_R1_LG388_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810297
title: ExtFig6B_RACE_STOML2_R1_LG388_annotated.pdf

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810297.v1
title: ExtFig6B_RACE_STOML2_R1_LG388_annotated.pdf

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810300.v1
title: ExtFig6B_RACE_STOML2_R3_LG395.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21815958.v1
title: ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.
DOI: 10.6084/m9.figshare.21810300
title: ExtFig6B_RACE_STOML2_R3_LG395.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810303
title: ExtFig6B_RACE_STOML2_R2_LG391.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810303.v1
title: ExtFig6B_RACE_STOML2_R2_LG391.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810306
title: ExtFig6B_RACE_STOML2_R2_LG391_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810306.v1
title: ExtFig6B_RACE_STOML2_R2_LG391_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX) or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21810246
title: ExtFig6A_RACE_BCAP31_R1_AI20.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
DOI: 10.6084/m9.figshare.21810246.v1
title: ExtFig6A_RACE_BCAP31_R1_AI20.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp, 51 bp, 27 bp or 15 bp insertions from the BCAP31 or SLC7A5 genes, and where indicated, with PR8 PA-X. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities