cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/vzqbn-pzn54
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-08-48_slicer6153.jpg: pilli ycw2014-03-08-48_slicer6154.jpg: unknown tube
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-48/rawdata/</p>20140308_YWC093_10049.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-48/rawdata/20140308_YWC093_10049.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140308_YWC093_10049_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-48/3dimage_42837/20140308_YWC093_10049_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kpt5n-x4291
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2013-08-20</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett, Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-14/rawdata/</p>20130820_Bdellovibrio+WM3433_10004.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-14/rawdata/20130820_Bdellovibrio+WM3433_10004.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130820_Bdellovibrio+WM3433_10004.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-14/3dimage_30285/20130820_Bdellovibrio+WM3433_10004.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p52me-dm785
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-25-87_slicer10220.jpg: Helicobacter pylori .  chemoreceptor .  flagellar motor ycw2013-10-25-87_slicer17908.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-87/rawdata/</p>20131025_Janelia_YWC043_20033.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-87/rawdata/20131025_Janelia_YWC043_20033.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_20033_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-87/3dimage_33562/20131025_Janelia_YWC043_20033_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.37kmgr
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Baileychromis centropomoides, Barboides gracilis, Barbopsis devecchii, Barbus hondeensis, Enteromius manicensis, Barbus kamaia, Enteromius motebensis, Barbus microbarbis, Labeobarbus microbarbis, Barbus microterolepis, Labeobarbus microterolepis, Barbus myersi, Enteromius radiatus, Barbus paucisquamatus, Labeobarbus paucisquamatus, Barbus tetraspilus, Barilius shariensis, Raiamas shariensis, Bathyaethiops breuseghemi, Bathyaethiops caudomaculatus, Bathyaethiops greeni, Bathybagrus grandis, Bathybagrus graueri, Bathybagrus platycephalus, Bathybagrus sianenna, Bathybagrus stappersii, Bathybagrus tetranema, Bathybates fasciatus, Bathybates ferox, Bathybates graueri, Bathybates hornii, Bathybates leo, Bathybates minor, Bathybates vittatus, Bathyclarias atribranchus, Bathyclarias euryodon, Bathyclarias filicibarbis, Bathyclarias foveolatus, Bathyclarias longibarbis, Bathyclarias nyasensis, Bathyclarias rotundifrons, Bathyclarias worthingtoni, Bathygobius casamancus, Bathygobius fuscus, Bathygobius petrophilus, Bathygobius samberanoensis, Bathygobius soporator, Bedotia albomarginata, Bedotia alveyi, Bedotia geayi, Bedotia leucopteron, Bedotia longianalis, Bedotia madagascariensis, Bedotia marojejy, Bedotia masoala, Bedotia tricolor, Belonoglanis brieni, Belonoglanis tenuis, Belonophago hutsebouti, Belonophago tinanti, Benitochromis batesii, Benitochromis conjunctus, Benitochromis finleyi, Benitochromis nigrodorsalis, Benitochromis riomuniensis, Benitochromis ufermanni, Benthochromis melanoides, Benthochromis tricoti, Boleophthalmus boddarti, Bostrychus africanus, Boulengerochromis microlepis, Boulengeromyrus knoepffleri, Brachyalestes bimaculatus, Brachyalestes carolinae, Brachyalestes comptus, Brachyalestes fwaensis, Brachyalestes imberi, Brycinus imberi, Brachyalestes jacksonii, Brachyalestes kingsleyae, Brachyalestes nurse, Brachypetersius altus, Brachypetersius cadwaladeri, Brachypetersius gabonensis, Brachypetersius huloti, Brachypetersius pseudonummifer, Brevimyrus niger, Brienomyrus brachyistius)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((56.2582 -7.6751,56.5989 -7.542,56.789 -7.1822,56.7426 -6.9401,55.8366 -6.9401,55.7807 -7.0342,55.8667 -7.4864,56.2582 -7.6751))"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006133-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/vkxkg-ncf55
title: Vibrio cholerae MKW1383

description: In the mixture with Bdellovibrio for 4 hr at 30C. Cells seemed lost PG layer without Bdellovibrio inside.
description: <strong>Tilt Series Date:</strong> 2013-10-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 4 hr at 30 C before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-42/rawdata/</p>montage_9x90015.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-42/rawdata/montage_9x90015.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_9x90015_part131_6.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-42/3dimage_32540/montage_9x90015_part131_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5ztnn-ach36
title: H. pylori 26695 / Hs.746T

description: Grid box YWC046
description: <strong>Tilt Series Date:</strong> 2013-11-05</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4.Hs.746T cells cultivated on QF Au finder grid for 1 day. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (100 ul gold resuspent in 30 ul). Manual blot by Whatman 1 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-05-25/rawdata/</p>20131104_YWC046_30009.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-05-25/rawdata/20131104_YWC046_30009.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131104_YWC046_30009_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-05-25/3dimage_34012/20131104_YWC046_30009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/604cf-0se24
title: H.pylori 26695 delta-cagH

description: Grid box: YWC090.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-02</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-23/rawdata/</p>20140302_YWC090_10023.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-23/rawdata/20140302_YWC090_10023.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140302_YWC090_10023.rec,  Reconstruction (Pixel Size 1.92 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-23/3dimage_39506/20140302_YWC090_10023.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7tjxf-0n306
title: H. pylori 26695 / Hs.746T

description: Grid box YWC057
description: <strong>Tilt Series Date:</strong> 2013-12-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-16/rawdata/</p>20131216_YWC057_10010.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-16/rawdata/20131216_YWC057_10010.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131216_YWC057_10010.rec,  Reconstruction (Pixel Size 1.92 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-16/3dimage_36138/20131216_YWC057_10010.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.uww94h
title: Occurrence Download

description: A dataset containing 4966 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Observation, Human Observation)",
 "Continent is Oceania",
 "Country is Australia",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Callicoma serratifolia",
 "Year is greater than or equal to 1900"
 ]
}

 The dataset includes 4966 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006134-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/0bb9e-kq106
title: H.pylori 26695 WT

description: Grid box YWC089.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-01-25_slicer7094.jpg: chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-25/rawdata/</p>20140301_YWC089_20017.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-25/rawdata/20140301_YWC089_20017.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140301_YWC089_20017_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-25/3dimage_39452/20140301_YWC089_20017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4aggc-8c740
title: NR1769/pKM101 conjugation

description: Grid box YWC118NR1769/pKM101 conjugating with NR1769 skinny E. coli
description: <strong>Tilt Series Date:</strong> 2014-09-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 185.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of E. coli donor NR1769 and recipient NR1769/pKM101 inoculated into fresh LB without antibiotics and incubated for 2.5 hours at 37 degrees and 200 rpm shaking.  Glow discharged Au finder grids placed carbon side up on LB 2% agar plate.  E. coli donor and recipient cultures pipetted onto grid in 1:1 ratio and placed in sealed chamber with a damp paper towel.  Plastic container placed inside 37C stationary incubator for 30 minutes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-27-14/rawdata/</p>20140927_YWC118_10003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-27-14/rawdata/20140927_YWC118_10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140927_YWC118_10003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-27-14/3dimage_46738/20140927_YWC118_10003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4a88j-hx204
title: Helicobacter pylori 26695

description: Cells are sick. Don't have flagella. Only see partial motors.
description: <strong>Tilt Series Date:</strong> 2013-10-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in Brucella broth (Fluka) + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-09-5_slicer10109.jpg: Helicobacter pylori .  chemoreceptors .  ribosome exclusion zone REZ
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-5/rawdata/</p>20131009_Hpylori_26695_BB_10005.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-5/rawdata/20131009_Hpylori_26695_BB_10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131009_Hpylori_26695_BB_10005_part131_15.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-5/3dimage_32140/20131009_Hpylori_26695_BB_10005_part131_15.rec"
        > <i class="download icon"></i> Download </a></p>