DOI: 10.5061/dryad.sk512
title: Data from: Collective cell migration without proliferation: density determines cell velocity and wave velocity

description: Collective cell migration contributes to embryogenesis, wound healing and
 tumor metastasis. Cell monolayer migration experiments help understanding
 what determines the movement of cells far from the leading edge.
 Inhibiting cell proliferation limits cell density increase and prevents
 jamming; we observe long-duration migration and quantify space-time
 characteristics of the velocity profile over large length- and
 time-scales. Velocity waves propagate backwards and their frequency
 depends only on cell density at the moving front. Both cell average
 velocity and wave velocity increase linearly with the cell effective
 radius regardless of the distance to the front. Inhibiting lamellipodia
 decreases cell velocity while waves either disappear or have a lower
 frequency. Our model combines conservation laws, monolayer mechanical
 properties and a phenomenological coupling between strain and polarity:
 advancing cells pull on their followers which then become polarized. With
 reasonable values of parameters, this model agrees with several of our
 experimental observations. Together, our experiments and model disantangle
 the respective contributions of active velocity and of proliferation in
 monolayer migration, explain how cells maintain their polarity far from
 the moving front, and highlight the importance of strain-polarity coupling
 and density in long-range information propagation.
description: Supporting Movie
 S1.Monolayer of MDCK cells
 migrating within a straight strip with mitomycin C to prevent divisions.
 Phase contrast image showing cell contours. Strip total length 4 mm, width
 1 mm. The first image, noted t = 0, is taken after around 5 h of
 migration. Duration of the movie: 26 h. Because of file size constraints,
 the movie resolution has been decreased, and the time interval between
 frame has been doubled (10 min instead of 5 min in the
 original).movie1.aviSupporting Movie S2.Same as Movie S1, nuclei labeled with histone GFP.movie2.aviSupporting Movie S3.Merge of phase-contrast image showing cell contours (Movie S1) in purple, and nuclei labeled with histone GFP (Movie S2) in green.movie3.aviSupporting Movie S4.Same as Movie S3, with original time interval between frame (5 min), zoomed on the front.movie4_zoom_front_df.aviSupporting Movie S5.Same as Movie S3, with original time interval between frame (5 min), zoomed on the middlemovie5_zoom_middle_df.aviSupporting Movie S6.Same as Movie S3, with original time interval between frame (5 min), zoomed on the back.movie6_zoom_back_df.aviSupporting Movie S7.Same as Movie S3 (time interval between frames: 6 min). After four hours (corresponding to the missing image at the 10th second of the current movie), CK666 is added; lamellipodia (both cryptic and front ones) are no longer detectable.movie7_CK666_20170908.avimitomycin_datamitomycin_data is a folder where velocity data are stored in the mitomycin drug case.kymographs_mitomycinkymographs_mitomycin is a folder where velocity kymographs are stored in the mitomycin case.ck666_datack666_data is a folder where velocity data are stored in the CK666 drug case.kymographs_CK666kymographs_CK666 is a folder where velocity kymographs are stored in the CK666 drug case.calculPhaseWavelet code