DOI: 10.6084/m9.figshare.788960
title: Localization and degradation data of the Arabidopsis borate transporter BOR1 in tobacco BY-2 cells

description: Borate effect on BY-2 growth.csv This is the dataset for Figure 2 in the associated article. Seven-day old tobacco BY-2 cells were subcultured into medium containing the indicated concentration of borate (mM) and shaken for a week. The volume of cells in the culture was measured after sedimentation of the cells by centrifugation. Packed cell volume indicate the actual volume of sedimented cells (ml) and the relative value represent the relative volume of the average of the triplicated experiment at 0.1 mM borate condition, which corresponds to the normal growth condition. Average of relative value and STDEV were used to make Figure 2. BOR1-GFP localization in BY-2 cells.csv This is the dataset for Figures 4C and 8B in the associated research article. The column A “cell type” indicates the cell line and collected image name. PG represents the PIP-GFP, WT represent the wild-type BOR-GFP, and mut represent the mutant BOR-GFP. The numbers after PG, WT and mut indicate the image number. Column B to I represent the actual data collected for the analysis. At each face of the cell images a box was made on the region corresponds to either of the face of the cells and the density of the intensity in the box was quantified (e.g. column B, A1). The same size box was copied close to the box at the outside of the cell and used to quantify the background intensity and the density of the intensity in the box was quantified (e. g. column C, A1 BG). Column J to M represent the subtracted density of fluorescence from the face to background (e. g. column B value minus column C value). The coumn N to Q represent the relative value of intensity to E1 face. The A1, A2, E1, E2 faces are indicated in Figure 4B. Borate medium conc BOR1-GFP degradation.csv This is the dataset for Figure 3C in the associated research article. Time course analysis of the degradation of BOR-GFP was quantified from the gel image. The column A indicate the experiment No. and the relative value of each experiments to time 0 as well as the average and standard deviation (STDEV) value of the relative intensity to time 0. From column B to K the top row indicate the time point and the other row indicate the actual and relative values, average and STDEV of relative to 0 time intensity. CHX BOR1-GFP degradation.csv This is the dataset for Figure 5B. Images were quantified from the gel and the relative value to without borate was calculated as described in the legend to . Column A is the experiment No. Colum B to I are the relative values of each treatment described in row A of the column. The numbers in the top row of columns B to I are the concentration of cyclohexamide (mM). Time course inhibitors BOR1-GFP degradation.csv This is the dataset for Figure 7B in the associated research article. In each experiment, to analyze the effect of TIBA or tyrphostin A23, a control without both borate and inhibitor was used. The relative value of the intensity of the bands were quantified as in the legend to . The values of row 1-4 are identical to the relative value of . Borate dependent degradation inhibition.csv This is the dataset for Figure 7A in the associated article. Column A indicates the experiment No. and the average and STDEV of the relative value. Column B indicates whether the data is the actual intensity or is the relative intensity to the values without both borate and inhibitors. The top row of column C to O indicates the presence and absence of borate and the names of inhibitors used. From the second to 25th row of columns C to O, the actual value as well as the relative values for without both borate and inhibitors were shown. Relative amount of Bor1-GFP at the addition of inhibitor (from time-course data).csv This is the background dataset for the processing of the data for Figure 7D in the associated research article. At the zero time or indicated time inhibitors were add to the culture. The relative intensity of bands to the start of the incubation with borate (0 time) is shown. Based on the average value in this dataset the relative value of the intensities of the bands in were calculated. Timimg of the addition of inhibitors (Fig 7D).csv This is the dataset for Figure 7D in the associated article. The relative values against each time points are shown. The time written in the first row indicates the timing of the addition of inhibitors. After calculation of the relative intensity to the time 0 without borate value as calculated in , the average and STDEV of three experiments were calculated. Thereafter these values were normalized to the average value calculated in (rows 7 and 8) and used to make Figure 7D.