DOI: 10.5061/dryad.x69p8czsf
title: Data from: A biological invasion modifies host immune responses to parasite infection

description: Biological invasions can disrupt the close and longstanding co-evolved
 relationships between host and parasites. At the same time, the shifting
 selective forces acting on demograpy during invasion can result in rapid
 evolution of traits in both host and parasite. Hosts at the invasion front
 may reduce investment into costly immune defences and redistribute those
 resources to other fitness-enhancing traits. Parasites at the invasion
 front may have reduced pathogenicity because traits that negatively impact
 host dispersal are left behind in the expanding range. The host’s immune
 system is its primary arsenal in the coevolutionary ‘arms race’ with
 parasites. To assess the effects of invasion history on immune responses
 to parasite infection we conducted a cross-infection experiment which
 paired common-garden reared cane toads and lungworm parasites originating
 from various sites in their invaded range across northern Australia.
 Infected toads had larger spleens and higher concentrations of eosinophils
 than did uninfected toads. Infected toads also exhibited lower
 bacteria-killing ability, perhaps reflecting a trade-off of resources
 towards defences that are more specifically anthelminthic. The impact of
 infection intensity on multiple immune measures differed among toads and
 parasites from different parts of the invasion trajectory, supporting the
 hypothesis that invasion has disrupted patterns of local adaptation.
description: Data are measures and identifying information for individual cane
 toads <em>(Rhinella marina</em>) experimentally infected with
 a lungworm parasite ( <em>Rhabdias
 p</em><em>seudosphaerocephala</em>.)
description: # Data from: A biological invasion modifies host immune responses to
 parasite infection
 [https://doi.org/10.5061/dryad.x69p8czsf](https://doi.org/10.5061/dryad.x69p8czsf) Description of the data and file structure Data are from an experiment where cane toads from different sites were exposed to lungworm parasites from different sites. Toad immune responses to infection were then measured. Cells with n/a indicate missing or undefined data. ID – Individual ID code  infection treatment- Control or exposed to parasites from one of 6 sites litter- ID code indicating family origin of each toad toad_site- location from which each toads parents were captured toad_state- Australian state each capture location was in Exposed to Rhabdias?- Yes vs No variable to indicate whether toad was exposed to Rhabdias or served as an unexposed Control worm_state- Australian state where the capture site of parasite donor toad was located became infected?- Yes vs No indication of whether parasites were present in the lungs at dissection immune assay date- date of euthanasia, dissection and immune assays SVL- snout to vent length (mm) measure of body size at time of euthanasia Mass- body mass (g) at time of euthanasia \#Rhabdias in lungs- Number of parasites in the lungs at dissection Spleen length mm- length of spleen at dissection Allen BKA index- Allen bacteria killing index (% change) mean luminesence RLU- Average luminescence reading during phagocytosis assay (relative luminescence units) log mn lumin for figure- log transformed mean luminescence wbc_conc- white blood cell concentration (cells/ml) Eo_conc- eosinophil concentration (cells/ml) Baso_conc- basophil concentration (cells/ml) Lympho_conc- lymphocyte concentration (cells/ml) Neut_conc- neutrophil concentration (cells/ml)