DOI: 10.5061/dryad.b2rbnzssx
title: Data from: Increased dietary levels of folic acid reduce survival and alter climbing behaviors 24 hours after hypoxia in male and female <em>Drosophila melanogaster</em>

description: Hypoxia is a major component of ischemic stroke. The prevalence of
 ischemic stroke is expected to increase as the global population ages and
 risk factors, like obesity, are on the rise. Nutrition is a modifiable
 risk factor for ischemic stroke. Increased dietary intake of folic acid
 (FA) has become an increasing problem in the U.S and other countries as
 more people are consuming at or above the recommended daily amount of FA.
 The impact of too much dietary FA on hypoxia is not well understood. This
 study aimed to investigate how increased dietary levels of FA impact
 hypoxia outcomes using Drosophila melanogaster as a model. Adult female
 and male w1118 Drosophila melanogaster flies were placed on control (CD)
 and 100 µM folic acid (FAD) diets. At 5 to 6 days old, progeny were
 exposed to hypoxia for two hours prior to returning to normoxic conditions
 to model reperfusion. We observed escape behavior in hypoxia larvae,
 confirming exposure to hypoxia. Using liquid chromatography-tandem mass
 spectrometry, elevated FA levels were observed in FAD compared to
 controls. We report increased acute hypoxia-induced mortality in FAD
 flies. Furthermore, FAD flies were not motivated to climb after hypoxia.
 Under normoxic conditions FAD flies had a higher velocity when descending
 during a climb. There was no impact of FA on neurodegeneration in brain
 tissue. The data collected from this study suggests that hypoxia has a
 negative effect on survival and climbing behavior in a fly model*.*
 Together these data suggest that increase dietary intake of FA can have
 negative health outcomes after hypoxia.
description: # Data from: Increased dietary levels of folic acid reduce survival and
 alters climbing behaviors 24 hours after hypoxia in male and female
 *Drosophila melanogaster*
 [https://doi.org/10.5061/dryad.b2rbnzssx\\](https://doi.org/10.5061/dryad.b2rbnzssx%5C) The dataset consists of five data files, each file is specific for each figure of the manuscript. All raw data has been provided. ## Description of the data and file structure Note that in the csv files and names, the following abbreviations are used: * Active caspase-3, ac3: marker of apoptosis * Control diet, CD: standard fly diet * Folic acid supplemented diet, FAD: Standard fly diet supplemented with 100µM of folic acid * Tetrahydrofolic acid, THF: folic acid metabolite * 5,10-methenyltetrahydrofolate, 5,10-CH-THF, folic acid metabolite **1. File List** * number_larvae_escape.csv: The number of larvae that escaped fly food at 0, 5, 10 minutes after being exposed to 1% hypoxia or normoxia conditions. * Whole_Body_Folic_acid_metabolites.csv: Measurements of folic acid, THF, and 5,10-CH-THF in whole fly bodies, all flies were from normoxic conditions * 24_hours_expired.csv: The percentage of flies expired 24 hours post hypoxia * climbing_assay.csv: Climbing assay behaviors including total movement, number of climbs, and velocity * active_caspase_3_relative_volume.csv: Relative volume of active-caspase 3 staining in male and female flies **2. Relationship between files**, if important: Different flies were used for all data sets. **3. Are there multiple versions of the dataset?** No ### **METHODOLOGICAL INFORMATION** **1. Description of methods used for collection/generation of data**: The data was collected in a laboratory setting. **1. Description of methods used for collection/generation of data**: **2. Methods for processing the data**: The data was analyzed in GraphPad Prism. **3. Instrument- or software-specific information needed to interpret the data**: Any graphing programing can used to graph and then interpret the data. **4. Describe any quality-assurance procedures performed on the data**: At data entry, the data was double-checked for accuracy. ### DATA-SPECIFIC INFORMATION FOR: number_larvae_escape.csv **1. Number of variables**: 3 **2. Number of cases/rows**: 32 **3. Variable List**: * Diet: Control diet: CD, Folic Acid Supplemented diet: FAD * Treatment: Normoxia, Hypoxia * Time: 0, 10, and 15 minutes * Number of larvae outside of food: The number of larvae that escaped from food in larvae at each timepoint (0,10, and 15 minutes), columns B to AD Empty cells in a dataset indicate not applicable ### **DATA-SPECIFIC INFORMATION FOR: Whole_Body_Folic_acid_metabolites.csv** **1. Number of variables**: 2 **2. Number of cases/rows**: 24 **3. Variable List**: * Diet: Control diet: CD, Folic Acid Supplemented diet: FAD * Sex: Female, Male * Metabolites (nmol/g): Folic Acid, THF, 5,10-CH-THF Empty cells in a dataset indicate not applicable ### DATA-SPECIFIC INFORMATION FOR: 24_hours_expired.csv * Diet: Control diet: CD, Folic Acid Supplemented diet: FAD * Treatment: Normoxia, Hypoxia * Number of expired flies: The number of expired at 24 hour time point post-hypoxia Empty cells in a dataset indicate not applicable. ### DATA-SPECIFIC INFORMATION FOR: climbing_assay.csv **1. Number of variables**: 5 **2. Number of cases/rows**: 120 to 149 **3. Variable List**: * Diet: Control diet: CD, Folic Acid Supplemented diet: FAD * Treatment: Normoxia, Hypoxia * Climbing Assay Measurements: * Total movement, number of movements made by each fly over 20 minutes * Number of climbs: the total number of climbs made by each fly over 20 minutes * Descending Velocity (mm/s), average speed of flies while they descend during climbing assay test. Empty cells in a dataset indicate not applicable. ### DATA-SPECIFIC INFORMATION FOR: active_caspase_3_relative_volume.csv **1. Number of variables**: 3 **2. Number of cases/rows**: 32 **3. Variable List**: * Diet: Control diet: CD, Folic Acid Supplemented diet: FAD * Sex: Female, Male * Active caspase 3 relative volume (µm3): Staining in brain tissue of flies exposed to hypoxia Empty cells in a dataset indicate not applicable. ## Code/Software Data was analyzed using GraphPad Prism.