DOI: 10.5061/dryad.2v0m8
title: Data from: Addicted? Reduced host resistance in populations with defensive symbionts

description: Heritable symbionts that protect their hosts from pathogens have been
 described in a wide range of insect species. By reducing the incidence or
 severity of infection, these symbionts have the potential to reduce the
 strength of selection on genes in the insect genome that increase
 resistance. Therefore, the presence of such symbionts may slow down the
 evolution of resistance. Here we investigated this idea by exposing
 Drosophila melanogaster populations to infection with the pathogenic
 Drosophila C virus (DCV) in the presence or absence of Wolbachia, a
 heritable symbiont of arthropods that confers protection against viruses.
 After nine generations of selection, we found that resistance to DCV had
 increased in all populations. However, in the presence of Wolbachia the
 resistant allele of pastrel—a gene that has a major effect on resistance
 to DCV—was at a lower frequency than in the symbiont-free populations.
 This finding suggests that defensive symbionts have the potential to
 hamper the evolution of insect resistance genes, potentially leading to a
 state of evolutionary addiction where the genetically susceptible insect
 host mostly relies on its symbiont to fight pathogens.
description: Genotype data of
 populations selected for virus resistanceColumns indicate the
 generation during selection, the Wolbachia infection status, the number of
 females with a given genotype (N_CC, N_CT, N_TT), the frequency of pastrel
 resistant allele (freq_C), the frequency of each genotype (freq_CC,
 freq_CT, freq_TT) and the number of individuals genotyped
 (N_individuals).Genotype_summary.txtGenotype data of control populations not exposed to the virusColumns indicate the generation during selection, the Wolbachia infection status, the number of females with a given genotype (N_CC, N_CT, N_TT), the frequency of pastrel resistant allele (freq_C), the frequency of each genotype (freq_CC, freq_CT, freq_TT) and the number of individuals genotyped (N_individuals).Genotype_summary_control.txtGenotype data of populations tested for DCV resistance after selectionColumns indicate the generation during selection, the Wolbachia infection status, the number of females with a given genotype (N_CC, N_CT, N_TT), the frequency of pastrel resistant allele (freq_C), the frequency of each genotype (freq_CC, freq_CT, freq_TT) and the number of individuals genotyped (N_individuals).Pastrel_Genotype_Phenotypic_assay.txtPhenotypic data on DCV resistance of populations after selectionColumns indicate the selection treatment, the Wolbachia infection status, the tetracycline treatment after selection, the infection treatment (stabbing with Ringer's solution or DCV), the replicate population, the replicate vial, the number of infected flies in a vial (N_flies) and the cumulative number of dead flies post-infection on a given day.Pastrel_phenotypic_assay.txtPhenotypic data on effect of infection procedure in control populationsColumns indicate the selection treatment, the Wolbachia infection status, the infection treatment (no stabbing, stabbing with Ringer's solution or DCV), the population, the replicate vial, the number of infected flies in a vial (N_flies) and the cumulative number of dead flies post-infection on a given day.pastrel_genotype_phenotype_survival.txtGenotype data of surviving flies 15 days post-infection in control populations (test of the infection procedure)Columns indicate the infection treatment, the Wolbachia infection status, the population, the number of females with a given genotype (N_CC, N_CT, N_TT), the frequency of pastrel resistant allele (freq_C), the frequency of each genotype (freq_CC, freq_CT, freq_TT) and the number of individuals genotyped (N_individuals).Genotype_summary_infection_procedure.txt