cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22081583
title: Indoquinoline Alkaloids from Sida rhombifolia (L.) (Malvaceae) and Antimicrobial Evaluation of Cryptolepinone Derivatives

description: Considering the relevance of natural products to the development of new drugs, this study focuses on the species Sida rhombifolia (L.), Malvaceae, widely used as a traditional remedy in several countries. The species is used to treat several diseases such as fevers, skin diseases, stomach pain, diarrhea, gum infection, conjunctivitis, urinary infections and inflammation. Many researchers around the word have investigated its pharmacological potential, and many uses have been confirmed. In spite of that, the phytochemical composition of its extracts is still poorly investigated. This research aims to make advances on the understanding of the constituents of S. rhombifolia identifying its alkaloidal compounds using ultra-high performance liquid chromatography coupled to mass spectrometry (UPLC-QTOF-MS/MS). By analyzing its alkaloidal fraction, it was possible to identify: 11-cryptolepine carboxylic acid (1), cryptolepine (2), quindoline (3), 11 methoxyquidoline (4), quindolinone (5), cryptolepinone (6), and 11-quindoline methyl ester (7). The alkaloids 1 and 7 are being reported for the first time from Sida genus. Based on the antimicrobial potential of cryptolepinone-type alkaloids, we prepared by semi-synthesis two cryptolepinone derivatives: 10-methylcryptolepinone (8) and the unreported compound 10-ethylcryptolepinone (9). Both derivatives were tested against fungal and bacterial strains and 10-methylcryptolepinone (8) showed strong activity against Cryptococcus neoformans, Candida albicans, Candida tropicalis, Aspergillus fumigatus and Aspergillus flavus.
DOI: 10.6084/m9.figshare.22081583.v1
title: Indoquinoline Alkaloids from Sida rhombifolia (L.) (Malvaceae) and Antimicrobial Evaluation of Cryptolepinone Derivatives

description: Considering the relevance of natural products to the development of new drugs, this study focuses on the species Sida rhombifolia (L.), Malvaceae, widely used as a traditional remedy in several countries. The species is used to treat several diseases such as fevers, skin diseases, stomach pain, diarrhea, gum infection, conjunctivitis, urinary infections and inflammation. Many researchers around the word have investigated its pharmacological potential, and many uses have been confirmed. In spite of that, the phytochemical composition of its extracts is still poorly investigated. This research aims to make advances on the understanding of the constituents of S. rhombifolia identifying its alkaloidal compounds using ultra-high performance liquid chromatography coupled to mass spectrometry (UPLC-QTOF-MS/MS). By analyzing its alkaloidal fraction, it was possible to identify: 11-cryptolepine carboxylic acid (1), cryptolepine (2), quindoline (3), 11 methoxyquidoline (4), quindolinone (5), cryptolepinone (6), and 11-quindoline methyl ester (7). The alkaloids 1 and 7 are being reported for the first time from Sida genus. Based on the antimicrobial potential of cryptolepinone-type alkaloids, we prepared by semi-synthesis two cryptolepinone derivatives: 10-methylcryptolepinone (8) and the unreported compound 10-ethylcryptolepinone (9). Both derivatives were tested against fungal and bacterial strains and 10-methylcryptolepinone (8) showed strong activity against Cryptococcus neoformans, Candida albicans, Candida tropicalis, Aspergillus fumigatus and Aspergillus flavus.
DOI: 10.6084/m9.figshare.22081586
title: Agronomic performance of Humuluslupulus L. varieties cultivated in organic and conventional systems in São Paulo center-west, Brazil

description: ABSTRACT: Hop is a multifunctional specie; however, a large part of its production is destined for the beer market. In Brazil, all hop demand is imported, which has aroused interest in national production. Genetic material and cultivation systems can influence hops production. Thus, this study evaluated morphological and productive performance of hop varieties grown under organic and conventional management in the central-west region of São Paulo, Brazil. The hop yard was installed in the experimental area of the FCA/UNESP (São Paulo State University, School of Agriculture) - Botucatu, in November 2018, the data were collected in second year of production. A randomized block design was adopted, in 2 x 5 Split-Plot, being the main factor: cultivation systems (organic and conventional), and secondary factor: hop varieties (Columbus, Chinook, Nugget, Cascade, and HallertauMittelfrüeh), with four blocks and four plants per plot. Crop-specific morphological and productive parameters were evaluated. The results showed no significant influence of cultivation systems in morphological parameters. Regarding the varieties, Chinook, Cascade, and Nugget stand out for cone length. Columbus, Chinook and Cascade showed higher yields when grown in organic system, obtaining 1100.66, 1088.27 and 940.40 g of cones per plant, respectively. While, HallertauMittelfrüeh variety was the least productive in both systems, in organic system with 160.50 g per plant production, and 267.84 g per plant in conventional system, and, also showing lower cycle compared to the others.
DOI: 10.6084/m9.figshare.22081586.v1
title: Agronomic performance of Humuluslupulus L. varieties cultivated in organic and conventional systems in São Paulo center-west, Brazil

description: ABSTRACT: Hop is a multifunctional specie; however, a large part of its production is destined for the beer market. In Brazil, all hop demand is imported, which has aroused interest in national production. Genetic material and cultivation systems can influence hops production. Thus, this study evaluated morphological and productive performance of hop varieties grown under organic and conventional management in the central-west region of São Paulo, Brazil. The hop yard was installed in the experimental area of the FCA/UNESP (São Paulo State University, School of Agriculture) - Botucatu, in November 2018, the data were collected in second year of production. A randomized block design was adopted, in 2 x 5 Split-Plot, being the main factor: cultivation systems (organic and conventional), and secondary factor: hop varieties (Columbus, Chinook, Nugget, Cascade, and HallertauMittelfrüeh), with four blocks and four plants per plot. Crop-specific morphological and productive parameters were evaluated. The results showed no significant influence of cultivation systems in morphological parameters. Regarding the varieties, Chinook, Cascade, and Nugget stand out for cone length. Columbus, Chinook and Cascade showed higher yields when grown in organic system, obtaining 1100.66, 1088.27 and 940.40 g of cones per plant, respectively. While, HallertauMittelfrüeh variety was the least productive in both systems, in organic system with 160.50 g per plant production, and 267.84 g per plant in conventional system, and, also showing lower cycle compared to the others.
DOI: 10.6084/m9.figshare.22081588
title: Effectiveness of oral electrolyte solutions with different compositions for the treatment of neonatal calves with induced osmotic diarrhea

description: ABSTRACT In a randomized controlled trial, we evaluated the effects of five oral electrolyte solutions (OESs) with different compositions of water, electrolyte, and acid-base balances of diarrheal neonatal calves. Osmotic diarrhea and dehydration were induced with sucrose in milk, spironolactone, and hydrochlorothiazide for 48 h in thirty 10-day old Holstein healthy calves with 43.5 ± 3.80 kg BW who were fed with natural milk. They were allocated to five treatment groups (n=6) based on the administered OES (commercial: OES A, B, C, D; and non-commercial: OES UEL). On the day of treatment, the calves received 6L of OES in two doses apart from milk intakes. Venous blood samples were collected at -48h (start of induction), -24h, 0h (start of the treatment day), 8h, 16h, 24h, 48h, and 72h. TPP, glucose, D-lactate, L-lactate, pH, pCO2, HCO3 -, BE, Na+, K+, Cl-, SID3, SIG, AG, Atot and percentage change in plasma volume (%PV) were measured or calculated and analyzed by two-way repeated measures ANOVA. All the calves developed osmotic diarrhea, mild to moderate dehydration, hyponatremia, relative hyperchloremia, and moderate to severe metabolic acidosis. The tested OESs were well accepted by the calves and effective in reversing dehydration, electrolyte imbalances, and metabolic acidosis. OES D did not completely correct hyponatremia, and SEO B caused transient hyperglycemia. It has been concluded that all the tested OESs are safe and effective for the treatment of diarrhea in calves with moderate degrees of dehydration and metabolic acidosis, which indicates that they have proper compositions for this purpose.
DOI: 10.6084/m9.figshare.22081588.v1
title: Effectiveness of oral electrolyte solutions with different compositions for the treatment of neonatal calves with induced osmotic diarrhea

description: ABSTRACT In a randomized controlled trial, we evaluated the effects of five oral electrolyte solutions (OESs) with different compositions of water, electrolyte, and acid-base balances of diarrheal neonatal calves. Osmotic diarrhea and dehydration were induced with sucrose in milk, spironolactone, and hydrochlorothiazide for 48 h in thirty 10-day old Holstein healthy calves with 43.5 ± 3.80 kg BW who were fed with natural milk. They were allocated to five treatment groups (n=6) based on the administered OES (commercial: OES A, B, C, D; and non-commercial: OES UEL). On the day of treatment, the calves received 6L of OES in two doses apart from milk intakes. Venous blood samples were collected at -48h (start of induction), -24h, 0h (start of the treatment day), 8h, 16h, 24h, 48h, and 72h. TPP, glucose, D-lactate, L-lactate, pH, pCO2, HCO3 -, BE, Na+, K+, Cl-, SID3, SIG, AG, Atot and percentage change in plasma volume (%PV) were measured or calculated and analyzed by two-way repeated measures ANOVA. All the calves developed osmotic diarrhea, mild to moderate dehydration, hyponatremia, relative hyperchloremia, and moderate to severe metabolic acidosis. The tested OESs were well accepted by the calves and effective in reversing dehydration, electrolyte imbalances, and metabolic acidosis. OES D did not completely correct hyponatremia, and SEO B caused transient hyperglycemia. It has been concluded that all the tested OESs are safe and effective for the treatment of diarrhea in calves with moderate degrees of dehydration and metabolic acidosis, which indicates that they have proper compositions for this purpose.
DOI: 10.6084/m9.figshare.22081589
title: Extruded urea could replace true protein source in supplements for lambs finished in tropical pastures

description: ABSTRACT The aim of this study was to evaluate the effects of replacing 50% of the true protein from soybean meal with extruded urea on the performance and carcass characteristics of lambs finished on pasture. Twenty lambs with initial weight of 29.9±6.2kg and 5 months of age, distributed in two treatments in blocks at random, were kept in pastures of Urochloa spp. Supplemented with 2% of body weight (BW). The treatments consisted of supplements containing soybean meal as the only true protein source (control), or extruded urea replacing 50% of soybean meal (NPN treatment). There was no effect of treatment on total supplement intake. There were differences in average daily gain (242.1 vs. 264.6g/day), slaughter weight (41.9 vs. 45.0kg) and carcass weight (18.9 vs. 20.1kg). There was no effect of the treatment on the final body condition score, hot carcass yield, carcass length, breast depth, subcutaneous fat thickness or rib eye area. The group of animals that received extruded urea showed superior carcass finishing characteristics (99.0% vs. 66.0%), maturity (22.0% vs. 0.0%), marbling (55.0% vs. 33.0%), meat texture (44.0% vs. 33.0%) and fat (66.0% vs. 22.0%). There were no significant effects on tenderness (7.5kg/f) or flesh color (L* = 33.2; a* = 16.2; b* = 8.3). There were no significant changes in the biochemical profile of the animals' blood during the experiment for both treatments. The replacement of 50% of protein from soybean meal by the extruded urea as NPN source in the supplement for lambs finished in Urochloa spp pastures provides better animal performance and qualitative characteristics of meat, without altering quantitative carcass characteristics.
DOI: 10.6084/m9.figshare.22081589.v1
title: Extruded urea could replace true protein source in supplements for lambs finished in tropical pastures

description: ABSTRACT The aim of this study was to evaluate the effects of replacing 50% of the true protein from soybean meal with extruded urea on the performance and carcass characteristics of lambs finished on pasture. Twenty lambs with initial weight of 29.9±6.2kg and 5 months of age, distributed in two treatments in blocks at random, were kept in pastures of Urochloa spp. Supplemented with 2% of body weight (BW). The treatments consisted of supplements containing soybean meal as the only true protein source (control), or extruded urea replacing 50% of soybean meal (NPN treatment). There was no effect of treatment on total supplement intake. There were differences in average daily gain (242.1 vs. 264.6g/day), slaughter weight (41.9 vs. 45.0kg) and carcass weight (18.9 vs. 20.1kg). There was no effect of the treatment on the final body condition score, hot carcass yield, carcass length, breast depth, subcutaneous fat thickness or rib eye area. The group of animals that received extruded urea showed superior carcass finishing characteristics (99.0% vs. 66.0%), maturity (22.0% vs. 0.0%), marbling (55.0% vs. 33.0%), meat texture (44.0% vs. 33.0%) and fat (66.0% vs. 22.0%). There were no significant effects on tenderness (7.5kg/f) or flesh color (L* = 33.2; a* = 16.2; b* = 8.3). There were no significant changes in the biochemical profile of the animals' blood during the experiment for both treatments. The replacement of 50% of protein from soybean meal by the extruded urea as NPN source in the supplement for lambs finished in Urochloa spp pastures provides better animal performance and qualitative characteristics of meat, without altering quantitative carcass characteristics.
DOI: 10.6084/m9.figshare.22081591
title: Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds

description: ABSTRACT Porcine hemoplasmosis is characterized as a geographically cosmopolitan disease caused by Mycoplasma suis and Mycoplasma parvum. Asymptomatic pigs are considered the focus of hemoplasmosis because they are carriers and reservoirs to new infections. This study aimed to determine the molecular occurrence of porcine hemoplasmas (PH) in the production cycle of technified farrow-to-finished swine herds. For this purpose, 20 swine herds were evaluated, where 501 whole blood samples were collected for qPCR and phylogenetic analyses for hemoplasmas. The epidemiological analysis was performed for the entire population and per the growth stage. The total prevalence for PH was 31.93% (161/501); 95% (19/20) of sampled herds were positive. The occurrence of PH by swine growth stages was nursery (30.47%), growing (31.29%), finishing (26.18%), and slaughter (40.25%). The quantification cycles (Cq) ranged from 3.18- 39.56 and the number of PH 16S rRNA copies per µL of DNA ranged from 5,57 x10-2 to 2.23 x1010. Sequencing and phylogenetic analysis of five selected samples showed 100% identity with M. parvum strain Indiana and two M. parvum sequences from Brazil/Goiás. This is the first report on PH in technified herds in Southeastern Brazil by growth stages.
DOI: 10.6084/m9.figshare.22081591.v1
title: Molecular prevalence of Mycoplasma parvum in production cycle of technified swine herds

description: ABSTRACT Porcine hemoplasmosis is characterized as a geographically cosmopolitan disease caused by Mycoplasma suis and Mycoplasma parvum. Asymptomatic pigs are considered the focus of hemoplasmosis because they are carriers and reservoirs to new infections. This study aimed to determine the molecular occurrence of porcine hemoplasmas (PH) in the production cycle of technified farrow-to-finished swine herds. For this purpose, 20 swine herds were evaluated, where 501 whole blood samples were collected for qPCR and phylogenetic analyses for hemoplasmas. The epidemiological analysis was performed for the entire population and per the growth stage. The total prevalence for PH was 31.93% (161/501); 95% (19/20) of sampled herds were positive. The occurrence of PH by swine growth stages was nursery (30.47%), growing (31.29%), finishing (26.18%), and slaughter (40.25%). The quantification cycles (Cq) ranged from 3.18- 39.56 and the number of PH 16S rRNA copies per µL of DNA ranged from 5,57 x10-2 to 2.23 x1010. Sequencing and phylogenetic analysis of five selected samples showed 100% identity with M. parvum strain Indiana and two M. parvum sequences from Brazil/Goiás. This is the first report on PH in technified herds in Southeastern Brazil by growth stages.
DOI: 10.6084/m9.figshare.22081595
title: Intestinal intussusception of Meckel’s diverticulum, a case report and literature review of the last five years

description: ABSTRACT Meckel’s diverticulum is the most common gastrointestinal tract anomaly. It arises from the incomplete closure of the omphalomesenteric conduit, which is a true diverticulum at the antimesenteric border of the ileum. Although the majority of patients are asymptomatic, they can present with inflammation, hemorrhage, intussusception, intestinal obstruction, and perforation, among others; this constitutes an important differential diagnosis for acute abdomen. A 19-year-old female sought medical attention because of intermittent diffuse abdominal pain for two months, nausea, and diarrhea. In the requested imaging tests, tomography, and enterotomography, a diagnosis of Meckel’s diverticulum with some degree of intussusception was suggested. The patient underwent elective surgical treatment without complications and was discharged on the second postoperative day with clinical improvement. In this section, we review publications on similar cases published in the last five years.
DOI: 10.6084/m9.figshare.22081595.v1
title: Intestinal intussusception of Meckel’s diverticulum, a case report and literature review of the last five years

description: ABSTRACT Meckel’s diverticulum is the most common gastrointestinal tract anomaly. It arises from the incomplete closure of the omphalomesenteric conduit, which is a true diverticulum at the antimesenteric border of the ileum. Although the majority of patients are asymptomatic, they can present with inflammation, hemorrhage, intussusception, intestinal obstruction, and perforation, among others; this constitutes an important differential diagnosis for acute abdomen. A 19-year-old female sought medical attention because of intermittent diffuse abdominal pain for two months, nausea, and diarrhea. In the requested imaging tests, tomography, and enterotomography, a diagnosis of Meckel’s diverticulum with some degree of intussusception was suggested. The patient underwent elective surgical treatment without complications and was discharged on the second postoperative day with clinical improvement. In this section, we review publications on similar cases published in the last five years.
DOI: 10.6084/m9.figshare.22081597
title: Is there an association between quadriceps muscle endurance and performance on activities of daily living in individuals with COPD?

description: Abstract Introduction Chronic obstructive pulmonary disease (COPD) is characterized by limited airflow associated with inflammatory response and systemic manifestations, such as dyspnea, as well as physical inactivity and intolerance to exercise. The sum of these changes can lead to peripheral muscle fatigue and exert an impact on the performance of activities of daily living (ADL). Objective To investigate the possible association between peripheral muscle fatigue and performance on ADL in individuals with COPD, and to compare the results to those of healthy age-matched individuals. Methods Individuals with a diagnosis of COPD and healthy volunteers aged 60 years or older were submitted to evaluations of peripheral muscle fatigue (using surface electromyography) and performance on the Glittre-ADL test. Results Nine individuals with COPD and ten controls were evaluated. Median isometric quadriceps contraction time was 72 [38] and 56 [51] seconds, respectively. Execution time on the ADL test was 6.1 [4] and 3.6 [1.3] minutes for COPD and control group respectively, with a significant difference between groups (p &lt; 0.05). However, no significant correlation was found between the evaluations. Conclusion No association was found between quadriceps muscle fatigue and performance on ADL in the sample studied. In the intergroup comparison, the individuals with COPD exhibited worse ADL time execution, but no significant difference was found regarding quadriceps muscle fatigue.
DOI: 10.6084/m9.figshare.22081597.v1
title: Is there an association between quadriceps muscle endurance and performance on activities of daily living in individuals with COPD?

description: Abstract Introduction Chronic obstructive pulmonary disease (COPD) is characterized by limited airflow associated with inflammatory response and systemic manifestations, such as dyspnea, as well as physical inactivity and intolerance to exercise. The sum of these changes can lead to peripheral muscle fatigue and exert an impact on the performance of activities of daily living (ADL). Objective To investigate the possible association between peripheral muscle fatigue and performance on ADL in individuals with COPD, and to compare the results to those of healthy age-matched individuals. Methods Individuals with a diagnosis of COPD and healthy volunteers aged 60 years or older were submitted to evaluations of peripheral muscle fatigue (using surface electromyography) and performance on the Glittre-ADL test. Results Nine individuals with COPD and ten controls were evaluated. Median isometric quadriceps contraction time was 72 [38] and 56 [51] seconds, respectively. Execution time on the ADL test was 6.1 [4] and 3.6 [1.3] minutes for COPD and control group respectively, with a significant difference between groups (p &lt; 0.05). However, no significant correlation was found between the evaluations. Conclusion No association was found between quadriceps muscle fatigue and performance on ADL in the sample studied. In the intergroup comparison, the individuals with COPD exhibited worse ADL time execution, but no significant difference was found regarding quadriceps muscle fatigue.