cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10296464.v1
title: Inhibitory effects of tanshinone IIA from Salvia miltiorrhiza Bge on human bladder cancer BIU-87 cells and xenograft in nude mice

description: Abstract The objective of this study was to investigate the inhibitory effects of tanshinone IIA on human bladder cancer BIU-87 cells and the xenograft in nude mice. BIU-87 cells were treated with tanshinone IIA with concentration of 0.5, 1, 2 and 4 g/L. The cell proliferation, cycle and apoptosis were detected. The nude mice with BIU-87 cell xenograft were treated with normal saline (control), 5-fluorouracil (5-FU), 200, 400 and 600 mg/kg tanshinone IIA, respectively. The animal body weight and growth of tumor were measured. The expressions of B-cell lymphoma-2 (Bcl-2), Caspase-3 and proliferating cell nuclear antigen (PCNA) protein in xenograft were detected. The apoptosis index of cells was determined. Results showed that, tanshinone IIA inhibited the proliferation of BIU-87 cells, promoted their apoptosis, and arrested more cells in G0/G1 phase. Tanshinone IIA significantly inhibited the growth of xenograft in nude mice and promoted the apoptosis of tumor cells in xenograft. Tanshinone IIA down-regulated Bcl-2 and PCNA expression and up-regulated Caspase-3 expression in xenograft. In conclusion, tanshinone IIA has inhibitory effects on human bladder cancer BIU-87 cells, and can inhibit the BIU-87 cells xenograft in nude mice.
DOI: 10.6084/m9.figshare.10296449
title: Soil microbial C:N:P ratio across physiognomies of Brazilian Cerrado Soil microbial biomass across a gradient of preserved native Cerrado

description: Abstract: Different physiognomies across the Cerrado could influence the microbial C:N:P ratio in the soil since these physiognomies present different abundance and diversity of plant species. Thus, the aim of this study was to evaluate the microbial C:N:P ratio in soil across three different physiognomies of Cerrado in the Northeast, Brazil, namely campo graminóide (dominance of grasses), cerrado stricto sensu (dominance of grasses, shrubs, low trees, and woody stratum), and cerradão (dominance of woody stratum). Campo graminóide was characterized by lower values of total organic C, N, microbial C:P, N:P, and soil C:N. Cerrado stricto sensu presented average values for most of the measured parameters, while cerradão presented higher values of microbial C, N, P, organic C, N and soil C:P and C:N ratios. The principal component analysis showed that the samples grouped according to the sites, with a clear gradient from campo graminóide to cerradão. Therefore, the differences of vegetation across physiognomies of Cerrado influenced the soil microbial C:N:P ratio, where cerradão showed highest microbial C:N:P ratio than soil under campo graminóide.
DOI: 10.6084/m9.figshare.10296455
title: Prediction of soil classes in a complex landscape in Southern Brazil

description: Abstract: The objective of this work was to evaluate the use of covariate selection by expert knowledge on the performance of soil class predictive models in a complex landscape, in order to identify the best predictive model for digital soil mapping in the Southern region of Brazil. A total of 164 points were sampled in the field using the conditioned Latin hypercube, considering the covariates elevation, slope, and aspect. From the digital elevation model, environmental covariates were extracted, composing three sets, made up of: 21 covariates, covariates after the exclusion of the multicollinear ones, and covariates chosen by expert knowledge. Prediction was performed with the following models: decision tree, random forest, multiple logistic regression, and support vector machine. The accuracy of the models was evaluated by the kappa index (K), general accuracy (GA), and class accuracy. The prediction models were sensitive to the disproportionate sampling of soil classes. The best predicted map achieved a GA of 71% and K of 0.59. The use of the covariate set chosen by expert knowledge improves model performance in predicting soil classes in a complex landscape, and random forest is the best model for the spatial prediction of soil classes.
DOI: 10.6084/m9.figshare.10296443
title: Is arbuscular mycorrhizal fungal species community affected by cotton growth management systems in the Brazilian Cerrado?

description: Abstract: Conventional cotton production in western Bahia, Brazil, involves intensive use of agricultural inputs and mechanization, which may affect arbuscular mycorrhizal fungi (AMF). This work aimed at studying the impact of conventional and organic cotton production in the AMF of western Bahia. Soil samples were obtained from conventional white cotton and colored cotton organic production systems as well as from native Cerrado areas, close to the white cotton fields, and from the subcaducifolia vegetation, close to the organic colored cotton farms. The most frequent species in the conventional farming areas belonged to the genera Acaulospora (10 spp.); Glomus (8 spp.); Dentiscutata (3 spp.); Ambispora, Pacispora and Scutellospora (2 spp. each), as well as Claroideoglomus etunicatum, Diversispora sp., Entrophospora infrequens, Gigaspora sp., Orbispora pernambucana, Paradentiscutata maritima, and Paraglomus occultum. Eighteen species were found in the organic farming areas, with the predominance of Glomus (5 spp.) and Acaulospora (5 spp.), and with Claroideoglomus, Dentiscutata, Gigaspora, Corymbiglomus, Orbispora, Paraglomus, Scutellospora, and Simiglomus (1 spp. each). Paraglomus bolivianum was first reported in Cerrado. In the native vegetation, nine species were found, with the predominance of Glomus and Acaulospora. The highest number of AMF species was found in the organic farming areas, which deserves further investigation.
DOI: 10.6084/m9.figshare.10296446
title: Performance of 'Valência' sweet orange grafted onto rootstocks in the state of Acre, Brazil

description: Abstract: The objective of this work was to select rootstocks, based on the agronomic characters and fruit quality of the 'Valência' orange tree. The evaluated rootstocks were: the 'Cleópatra' mandarin; the TSKFL x CTTR-013, LVK x LCR-038, TSKC x CTQT 1439-004, LVK x LVA-009, TSK x TRENG-256 ('Indio' citrandarin) hybrid genotypes; the 'Santa Cruz' Rangpur lime; and the TSKFL x CTC-25-002, and TSKC x CTSW-038 hybrids. The experiment was installed in 2010 and evaluated from 2013 to 2017. The parameters plant height, canopy volume, yield efficiency, scion:rootstock ratio, survival percentage, and drought tolerance were evaluated in 2017. The yield average of five harvests was estimated, as well as the annual production per plant and the accumulated production. The analyzed fruit traits were: mass, diameter, peel length and thickness, juice yield, soluble solids, acidity, and technological index. 'Valência' shows a lower plant height and canopy volume, besides a higher yield efficiency, when grafted on TSKC x CTSW-038 than on the other rootstocks. LVK x LCR-038, TSKC x CTQT 1439-004, 'Indio' citrandarin, and 'Santa Cruz' Rangpur lime increase the production of the 'Valência' orange. LVK x LCR-038 and the 'Indio' citrandarin induce the scion to produce a better quality fruit.
DOI: 10.6084/m9.figshare.10296452
title: Probiotic, antibiotic and combinations in Nile tilapia juveniles culture

description: Abstract: This study aimed to evaluate the effect of the commercial probiotic (Saccharomyces cerevisiae), antibiotic (florfenicol), and its combination for Nile tilapia culture. The experiment was arranged in a completely randomized design with five replications, and five treatments diets: Control: water and diet without additives; YD: yeast in the diet (1 g Kg-1); AW: antibiotic in the water (0.002 g L-1); AWYD: antibiotic in the water, and yeast in the diet (0.002 g L-1 and 1 g Kg-1); and AD: antibiotic in the diet (0.01 g kg-1). The growth parameters as total and standard length, weight, weight gain, biomass, Fulton’s condition factor, specific growth rate and plasma cortisol of tilapia did not show the difference between the treatments. The survival rates and food conversion rate of fish were greater in treatment with florfenicol included in the diet. The commercial probiotic did not improve growth or survival. The administration of the antibiotic florfenicol in the water needs more studies. The inclusion of the antibiotic in the diet promotes growth and survival in Nile tilapia juvenile.
DOI: 10.6084/m9.figshare.10296455.v1
title: Prediction of soil classes in a complex landscape in Southern Brazil

description: Abstract: The objective of this work was to evaluate the use of covariate selection by expert knowledge on the performance of soil class predictive models in a complex landscape, in order to identify the best predictive model for digital soil mapping in the Southern region of Brazil. A total of 164 points were sampled in the field using the conditioned Latin hypercube, considering the covariates elevation, slope, and aspect. From the digital elevation model, environmental covariates were extracted, composing three sets, made up of: 21 covariates, covariates after the exclusion of the multicollinear ones, and covariates chosen by expert knowledge. Prediction was performed with the following models: decision tree, random forest, multiple logistic regression, and support vector machine. The accuracy of the models was evaluated by the kappa index (K), general accuracy (GA), and class accuracy. The prediction models were sensitive to the disproportionate sampling of soil classes. The best predicted map achieved a GA of 71% and K of 0.59. The use of the covariate set chosen by expert knowledge improves model performance in predicting soil classes in a complex landscape, and random forest is the best model for the spatial prediction of soil classes.
DOI: 10.6084/m9.figshare.10296440
title: Novel homozygous mutation in a colombian patient with persistent müllerian duct syndrome: expanded phenotype

description: ABSTRACT The anti-Müllerian hormone triggers the regression of uterus and fallopian tubes in male embryos; if there are problems in the synthesis or action of this protein, Müllerian structures persist in an otherwise phenotypic male. The most frequent clinical presentation of Persistent Mullerian Duct syndrome is cryptorchidism and inguinal hernia. The few cases reported in adults are incidental findings or inguinal hernias. However, we present an adult male with history of bilateral cryptorchidism with unsuccessful orchidopexy, who presents with a large abdominal mass with the finding of a seminomatous tumor and persistence of Müllerian structures, in whom the variant c.916delC (p.Leu306Cysfs*29) in the AMHR2 gene not previously reported was documented.
DOI: 10.6084/m9.figshare.10296443.v1
title: Is arbuscular mycorrhizal fungal species community affected by cotton growth management systems in the Brazilian Cerrado?

description: Abstract: Conventional cotton production in western Bahia, Brazil, involves intensive use of agricultural inputs and mechanization, which may affect arbuscular mycorrhizal fungi (AMF). This work aimed at studying the impact of conventional and organic cotton production in the AMF of western Bahia. Soil samples were obtained from conventional white cotton and colored cotton organic production systems as well as from native Cerrado areas, close to the white cotton fields, and from the subcaducifolia vegetation, close to the organic colored cotton farms. The most frequent species in the conventional farming areas belonged to the genera Acaulospora (10 spp.); Glomus (8 spp.); Dentiscutata (3 spp.); Ambispora, Pacispora and Scutellospora (2 spp. each), as well as Claroideoglomus etunicatum, Diversispora sp., Entrophospora infrequens, Gigaspora sp., Orbispora pernambucana, Paradentiscutata maritima, and Paraglomus occultum. Eighteen species were found in the organic farming areas, with the predominance of Glomus (5 spp.) and Acaulospora (5 spp.), and with Claroideoglomus, Dentiscutata, Gigaspora, Corymbiglomus, Orbispora, Paraglomus, Scutellospora, and Simiglomus (1 spp. each). Paraglomus bolivianum was first reported in Cerrado. In the native vegetation, nine species were found, with the predominance of Glomus and Acaulospora. The highest number of AMF species was found in the organic farming areas, which deserves further investigation.
DOI: 10.6084/m9.figshare.10296452.v1
title: Probiotic, antibiotic and combinations in Nile tilapia juveniles culture

description: Abstract: This study aimed to evaluate the effect of the commercial probiotic (Saccharomyces cerevisiae), antibiotic (florfenicol), and its combination for Nile tilapia culture. The experiment was arranged in a completely randomized design with five replications, and five treatments diets: Control: water and diet without additives; YD: yeast in the diet (1 g Kg-1); AW: antibiotic in the water (0.002 g L-1); AWYD: antibiotic in the water, and yeast in the diet (0.002 g L-1 and 1 g Kg-1); and AD: antibiotic in the diet (0.01 g kg-1). The growth parameters as total and standard length, weight, weight gain, biomass, Fulton’s condition factor, specific growth rate and plasma cortisol of tilapia did not show the difference between the treatments. The survival rates and food conversion rate of fish were greater in treatment with florfenicol included in the diet. The commercial probiotic did not improve growth or survival. The administration of the antibiotic florfenicol in the water needs more studies. The inclusion of the antibiotic in the diet promotes growth and survival in Nile tilapia juvenile.
DOI: 10.6084/m9.figshare.10296434
title: Avocado oil as an inducer of the extracellular lipase activity of Kluyveromyces marxianus L-2029

description: Abstract Avocado represents one of the most nutritious and consumed foods in Mexico, and its export generates significant profits; because of its fatty acids content, the avocado oil was used as an inducer for the synthesis of extracellular lipases from the non-conventional yeast Kluyveromyces marxianus L-2029. Lipases are able to catalyze acyl glycerides, hydrolyzing them into fatty acids and glycerol; they have several applications in various industries. In order to determine the ideal initial avocado oil concentration in the culture medium for the yeast, the induction was carried out at different concentrations in incubation for 24 h. The yeast presented the greater extracellular lipase productivity at a 3.5% v/v avocado oil concentration, with a 3.47 μmol PNF/mg prot * min (U/mL) maximum activity. The enzymatic extract obtained at this condition had an optimum lipase activity temperature of 36 °C and pH 6. The pYJR10W lipase was detected in the enzymatic extract from avocado oil induction, and is therefore responsible for the extracellular lipase activity of K. marxianus.
DOI: 10.6084/m9.figshare.10296437
title: Exploring consumers’ knowledge and perceptions of unconventional food plants: case study of addition of Pereskia aculeata Miller to ice cream

description: Abstract Unconventional food plants (UFPs) are by definition little known and infrequently consumed by population, although are recognized as having high nutritional value and economic potential. The objective was to assess the knowledge about UFPs by the population in the southwestern region of the state of Paraná, Brazil; and more particularly to evaluate the holistic perception of an ice cream prepared with the UFP ora-pro-nóbis, a plant that is gaining attention in recent years We applied a questionnaire to assess knowledge of several UFPs; check-all-that-apply questions to evaluate the knowledge, habits and perceptions regarding UFPs; and word association (WA) and acceptance tests for holistic and hedonic evaluation of the ice cream. The results revealed that the best known UFP species were taro and roselle, followed by ora-pro-nóbis and lamb’s ear. They also revealed that among participants, the majority (63%) had heard of UFPs, but only 19.4% stated they had the custom of eating these plants. The respondents also indicated interest in buying UFPs, but stated it was not easy to find them for sale. In the WA test, ice cream was described mainly in the categories tasty, innovation, sweet taste, plant flavor, smooth and creamy, and the acceptance index was 74%.
DOI: 10.6084/m9.figshare.10296449.v1
title: Soil microbial C:N:P ratio across physiognomies of Brazilian Cerrado Soil microbial biomass across a gradient of preserved native Cerrado

description: Abstract: Different physiognomies across the Cerrado could influence the microbial C:N:P ratio in the soil since these physiognomies present different abundance and diversity of plant species. Thus, the aim of this study was to evaluate the microbial C:N:P ratio in soil across three different physiognomies of Cerrado in the Northeast, Brazil, namely campo graminóide (dominance of grasses), cerrado stricto sensu (dominance of grasses, shrubs, low trees, and woody stratum), and cerradão (dominance of woody stratum). Campo graminóide was characterized by lower values of total organic C, N, microbial C:P, N:P, and soil C:N. Cerrado stricto sensu presented average values for most of the measured parameters, while cerradão presented higher values of microbial C, N, P, organic C, N and soil C:P and C:N ratios. The principal component analysis showed that the samples grouped according to the sites, with a clear gradient from campo graminóide to cerradão. Therefore, the differences of vegetation across physiognomies of Cerrado influenced the soil microbial C:N:P ratio, where cerradão showed highest microbial C:N:P ratio than soil under campo graminóide.
DOI: 10.6084/m9.figshare.10296431
title: Quality enhancement of fermented vegetable juice by probiotic through fermented yam juice using Saccharomyces cerevisiae

description: Abstract As the demand for probiotic foods continues to increase and lactose intolerance is common, it is necessary to develop a range of non-milk probiotic beverages. The purpose of this study was to evaluate the preparation process of fermented probiotic beverages using various vegetables as raw materials, and to evaluate the chemical and organoleptic properties of the mixed fermented vegetable juices. The mixed fermented vegetable juice was a beverage obtained by mixing the vegetable juice (purple cabbage, tomato and carrot) fermented by Lactobacillus plantarum and the yam juice fermented by the yeast in an appropriate ratio. During lactobacillus fermentation (48 hours at 30 °C), the cell count of Lactobacillus plantarum increased from 7.49 ± 0.21 log CFU/mL to 9.13 ± 0.19 log CFU/mL (p &lt; 0.05), the glucose decreased from 8.924 g/L to 5.528 g/L, and the pH decreased from 5.03 to 3.62. Oxidation activity slightly decreased. The lactic acid and titratable acid/total acid content increased, and the trend of change was similar. During the yeast fermentation (28 °C, 24h), the glucose was almost depleted and ethanol was accumulated. GC-MS analysis showed that microbial fermentation had a significant effect on volatile components. The sensory evaluation results showed that the proper mixing of fermented vegetable juice and fermented yam juice had a significant increase in taste, odor and overall acceptance of the product. The mixed fermented vegetable juice has the potential to adapt to the consumer groups and has in-depth research value.
DOI: 10.6084/m9.figshare.10296437.v1
title: Exploring consumers’ knowledge and perceptions of unconventional food plants: case study of addition of Pereskia aculeata Miller to ice cream

description: Abstract Unconventional food plants (UFPs) are by definition little known and infrequently consumed by population, although are recognized as having high nutritional value and economic potential. The objective was to assess the knowledge about UFPs by the population in the southwestern region of the state of Paraná, Brazil; and more particularly to evaluate the holistic perception of an ice cream prepared with the UFP ora-pro-nóbis, a plant that is gaining attention in recent years We applied a questionnaire to assess knowledge of several UFPs; check-all-that-apply questions to evaluate the knowledge, habits and perceptions regarding UFPs; and word association (WA) and acceptance tests for holistic and hedonic evaluation of the ice cream. The results revealed that the best known UFP species were taro and roselle, followed by ora-pro-nóbis and lamb’s ear. They also revealed that among participants, the majority (63%) had heard of UFPs, but only 19.4% stated they had the custom of eating these plants. The respondents also indicated interest in buying UFPs, but stated it was not easy to find them for sale. In the WA test, ice cream was described mainly in the categories tasty, innovation, sweet taste, plant flavor, smooth and creamy, and the acceptance index was 74%.
DOI: 10.6084/m9.figshare.10296440.v1
title: Novel homozygous mutation in a colombian patient with persistent müllerian duct syndrome: expanded phenotype

description: ABSTRACT The anti-Müllerian hormone triggers the regression of uterus and fallopian tubes in male embryos; if there are problems in the synthesis or action of this protein, Müllerian structures persist in an otherwise phenotypic male. The most frequent clinical presentation of Persistent Mullerian Duct syndrome is cryptorchidism and inguinal hernia. The few cases reported in adults are incidental findings or inguinal hernias. However, we present an adult male with history of bilateral cryptorchidism with unsuccessful orchidopexy, who presents with a large abdominal mass with the finding of a seminomatous tumor and persistence of Müllerian structures, in whom the variant c.916delC (p.Leu306Cysfs*29) in the AMHR2 gene not previously reported was documented.
DOI: 10.6084/m9.figshare.10296446.v1
title: Performance of 'Valência' sweet orange grafted onto rootstocks in the state of Acre, Brazil

description: Abstract: The objective of this work was to select rootstocks, based on the agronomic characters and fruit quality of the 'Valência' orange tree. The evaluated rootstocks were: the 'Cleópatra' mandarin; the TSKFL x CTTR-013, LVK x LCR-038, TSKC x CTQT 1439-004, LVK x LVA-009, TSK x TRENG-256 ('Indio' citrandarin) hybrid genotypes; the 'Santa Cruz' Rangpur lime; and the TSKFL x CTC-25-002, and TSKC x CTSW-038 hybrids. The experiment was installed in 2010 and evaluated from 2013 to 2017. The parameters plant height, canopy volume, yield efficiency, scion:rootstock ratio, survival percentage, and drought tolerance were evaluated in 2017. The yield average of five harvests was estimated, as well as the annual production per plant and the accumulated production. The analyzed fruit traits were: mass, diameter, peel length and thickness, juice yield, soluble solids, acidity, and technological index. 'Valência' shows a lower plant height and canopy volume, besides a higher yield efficiency, when grafted on TSKC x CTSW-038 than on the other rootstocks. LVK x LCR-038, TSKC x CTQT 1439-004, 'Indio' citrandarin, and 'Santa Cruz' Rangpur lime increase the production of the 'Valência' orange. LVK x LCR-038 and the 'Indio' citrandarin induce the scion to produce a better quality fruit.
DOI: 10.6084/m9.figshare.10296425
title: Partial Congenital Absence of The Pericardium: A Case Report

description: Abstract The complete or the partial absence of pericardium is a rare congenital malformation for which the patients are commonly asymptomatic and the diagnosis is incidental. The absence of the left side of the pericardium is the most common anomaly that is reported in the literature while the complete absence of pericardium or the absence of the right side of the pericardium are uncommon and their criteria are still unrecognized given their rare occurrence in clinical practice. This paper aims to report a case of 19-year-old male with the congenital partial absence of both sides of the pericardium and to highlight the symptoms and the different cardiac imaging modalities used to confirm the diagnosis of this defect.