DOI: 10.5061/dryad.3vr00
title: Data from: Multiple strong postmating and intrinsic postzygotic reproductive barriers isolate florally diverse species of Jaltomata (Solanaceae)

description: Divergence in phenotypic traits often contributes to premating isolation
 between lineages, but could also promote isolation at postmating stages.
 Phenotypic differences could directly result in mechanical isolation or
 hybrids with maladapted traits; alternatively, when alleles controlling
 these trait differences pleiotropically affect other components of
 development, differentiation could indirectly produce genetic
 incompatibilities in hybrids. Here, we determined the strength of 9
 postmating and intrinsic postzygotic reproductive barriers among 10
 species of Jaltomata (Solanaceae), including species with highly divergent
 floral traits. To evaluate the relative importance of floral trait
 diversification on the strength of these postmating barriers, we assessed
 their relationship to floral divergence, genetic distance, geographical
 context, and ecological differences, using conventional tests and a new
 linear mixed modeling approach. Despite close evolutionary relationships,
 all species pairs showed moderate to strong isolation. Nonetheless, floral
 trait divergence was not a consistent predictor of the strength of
 isolation; instead this was best explained by genetic distance, although
 we found evidence for mechanical isolation in one species, and an overall
 positive relationship between floral trait divergence and fruit set
 isolation across species pairs. Overall, our data indicate that intrinsic
 postzygotic isolation is more strongly associated with genome-wide genetic
 differentiation, rather than floral divergence.
description: Crossing Data used to
 Calculate Isolation ValuesThis file contains data
 obtained from controlled hybridizations (crosses) in the greenhouse, among
 different Jaltomata species and their F1 hybrids. These data were used to
 calculate measures of reproductive isolation for the following stages:
 fruit set, fruit size, seed set, viable seed set, F1 fruit set, F1 fruit
 size, and F1 seed set.CrossingData.csvMeasured Floral Color Attributes used to Calculate Floral Distances MetricsThis file contains measures of color attributes for petal and nectar color. For each color trait, images were standardized for lighting, and then different color attributes were measured in ImageJ. These data were used to calculate floral distance metrics.Floral_ColorData.csvMeasured Floral Morph Traits used to Calculated Floral Distance MetricsThis file contains measures of floral organs and nectar volume, which were collected on fresh mature flowers. These data were used to calculate floral distance metrics.Floral_MorphData.csvPollen Growth Data used to Calculate Pollen-Pistil IsolationThis file contains data obtained from pollen growth assays, which were used to determine extent of pollen-pistil isolation. It includes species pairs, individuals used, pollination and style collection dates, and pollen tube length information.PollenGrowthAssayData.csvPollen Viability Data used to Calculate F1 Hybrid Pollen IsolationThis file contains pollen count data, which were used to determine F1 pollen fertility isolation. Pollen counts were obtained using aniline-blue stain.PollenViabilityData.csvLoci used to Estimate Genetic Distance among SpeciesThis is a compressed file containing alignments for the 60 loci used to estimate genetic distance among Jaltomata species.GeneticDistanceLoci.gzShort Description FileThis is a readme file containing descriptions of the included data files.