DOI: 10.6084/m9.figshare.11513244.v1
title: Ruiz-Carrera, Violeta (2020). Tolerance to Nitrogen ions enrichment in a Gulf of Mexico's freshwater submerged grass. Database

description: Subject of study: Wild ecotype <i>Vallisneria americana</i> Michx. (Hydrocharitaceae) from a tropical wetlands from Central America. The data show conditions and results of the evaluation experimental of four Nitrogen (N) concentrations (500 to 2000 µgL<sup>-1</sup>) and three N sources (NH<sub>4</sub>, NO<sub>3</sub>, 1: 1 NH<sub>4</sub>: NO<sub>3</sub> ratio) in an overlaying aqueous phase, using local tap water as control (174 µgL<sup>-1</sup> Total N) in rooted autotrophic juvenile <i>Vallisneria americana</i>, established <i>in vitro</i> on in two-phase culture medium. The treatments ranged from 7 to 111 µM TN (N = 13). Dry biomass of lyophilized leaf and root and N contents (gas and high-performance liquid chromatography) were analyzed to estimate the N-NH<sub>4</sub>, N-NO<sub>3</sub> and N-NO<sub>2</sub> accumulations, as well as, the variation due to the effect of the evaluated factors on total biomass, pH and either conductivity or ionic strength on the spent aqueous medium. Extractions of three N inorganic ions, N-NH<sub>4</sub>, N-NO<sub>3</sub> and N-NO<sub>2</sub> in tissue samples were carried out in mortar with methanol (HPLC grade) at 60% dissolved in deionized water. The N accumulation curves were obtained with each ion by tissue (N endogenous, µmolg<sup>-1</sup>) <i>versus</i> increment of concentrations of the N source supplied (N exogenous, µM).<br>ReferencesBautista-Regil J, Ruiz-Carrera V, Sánchez AJ, Salcedo-Meza MA, Florido R (2016) Stress to microscale of Vallisneria americana by enrichment of nutrients with stoichiometry N:P. Hidrobiológica 26(1):53-60. https://doi.org/10.24275/uam/izt/dcbs/hidro/2016v26n1/Ruiz Cao T, Xie P, Ni L, Wu A, Zhang M, Wu S, Smolders AJP (2007) The role of NH4+ toxicity in the decline of the submersed macrophyte Vallisneria natans in lakes of the Yangtze River basin, China. Mar Fresh Res 58:581-587. doi:10.1071/MF06090 Cao T, Xie P, Li Z, Ni L, Zhang M, Xu J (2009) Physiological stress of high NH4+ concentration in water column on the submersed macrophyte Vallisneria natans L.. Bull Environ Contam Toxicol 82(3):296-299. doi:10.1007/s00128-008-9531-5 APHA (2005) Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association/American Water Works Association/Water Environment Federation, Washington DC. Husted S, Hebbern C, Mattsson M, Schjoerring JK (2001) A critical experimental evaluation of methods for determination of NH4+ in plant tissue, xylem sap and apoplastic fluid. Physiol Plant 109:167–179. doi:10.1034/j.1399-3054.2000.100209.x Lauridsen TL, Jeppesen E, Declerck SA, De Meester L, Conde-Porcuna JM, Rommens W, Brucet S (2015) The importance of environmental variables for submerged macrophyte community assemblage and coverage in shallow lakes: differences between northern and southern Europe. Hydrobiologia 744(1):49-61. doi:10.1007/s10750-014-2055-6 Le Moal M, Gascuel-Odoux C, Ménesguen A, Souchon Y, Étrillard C, Levain A, Pinay G (2019) Eutrophication: a new wine in an old bottle?. Sci Total Environ 651:1-11. doi:10.1016/j.scitotenv.2018.09.139 Leoni V, Vela A, Pasqualini V, Pergent-Martini CG, Pergent G (2008) Effects of experimental reduction of light and nutrient enrichments (N and P) on seagrasses: a review. Aquat Conserv 18:202–220. doi:10.1002/aqc.842 Lulla H, Chen SS, Sena FJ (2006) Simultaneous determination of nitrate and nitrite in toothpastes by high-performance liquid chromatography. J Pharm Sci 73(7):1004-1006. doi:10.1002/jps.2600730740. Sánchez AJ, Florido R, Salcedo MA, Ruiz-Carrera V, Montalvo-Urgel H, Raz-Guzman A (2012) Macrofaunistic diversity in Vallisneria americana Michx. in a tropical wetland, Southern Gulf of Mexico. A. Mahamane (Ed.), Diversity of ecosystems, InTech, Zagreb (2012), pp. 1-26 In: Mahamane A (ed.). Diversity of Ecosystems, In Tech, Zagreb, pp. 1-26. doi:10.5772/35331 Viana IG, Saavedra‐Hortúa DA, Mtolera M, Teichberg M (2019) Different strategies of nitrogen acquisition in two tropical seagrasses under nitrogen enrichment. New Phytol 223(3):1217-1229 doi:10.1111/nph.15885 Wang Y, Gao G, Qin B, Wang X (2012) Ecophysiological and anatomical responses of Vallisneria natans to nitrogen and phosphorus enrichment. Knowl. Managt. Aquatic Ecosyst, 405(05). Yang XE, Wu X, Hao HL, He ZL (2008) Mechanisms and assessment of water eutrophication. J Zhejiang Univ Sci B, 9(3):197-209. doi:10.1631/jzus.B0710626<br>