cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/a0n4r-22098
title: Agrobacterium tumefaciens C58

description: Wild type
description: <strong>Tilt Series Date:</strong> 2014-01-28</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on plates, scraped off and grown o/n in a drop</p>
<strong>Sample Preparation:</strong> Vitrobot, manual blot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-28-9/rawdata/</p>c58_drop0002.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-28-9/rawdata/c58_drop0002.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jzvan-yfa72
title: #1-60 BaxH6 Lysogen Ecoli phage

description: Bax/Holin negative controlBaxH6 Lysogen Ecoli lamdaphageMC4100[lamdadelta(SR)]
description: <strong>Tilt Series Date:</strong> 2013-07-11</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Xu Luo and Xiaming Pangxuluo@unmc.edu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> see Xiaming notes</p>
<strong>Sample Preparation:</strong> Titan 26k, 7/10/13expo 0.9  150e-poor fuducials</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-11-12/rawdata/</p>1-600011.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-11-12/rawdata/1-600011.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5zvf3-nq562
title: #4-60 BaxH6 Lysogen Ecoli phage

description: Bax delta H6 bigger hole Bax mutantBaxH6 Lysogen Ecoli lamdaphageMC4100[lamdadelta (SR)]
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor failed</p>
<strong>Collaborators and Roles:</strong> Xo Luo and Xiaming Pang  xuluo@unmc.edu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> see Xiamings notes</p>
<strong>Sample Preparation:</strong> Titan 26k 7/11/13  expo 0.9 150e- poor fudicials</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-12-14/rawdata/</p>B1_4-600014.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-12-14/rawdata/B1_4-600014.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yn0mp-dxc52
title: Hylemonella gracilis

description: Hylemonella gracilis.  The rest of the files are in ~/Images/060816.  This date's files are not good enough for motor averaging.
description: <strong>Tilt Series Date:</strong> 2006-08-16</p>
<strong>Data Taken By:</strong> Gavin Murphy</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> Cells provided by Jared Leadbetter; Data collected and processed by Gavin Murphy</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in dilute media without salt.  0.5 g/L of Tryptone, 0.5 g/L YE. Cells grown for 2 days.  OD never gets above 0.005.  Centrifuged lightly, then concentrated 100x fold.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2006-08-16-8/rawdata/</p>S5_S474G3_110e10d22k.mrc,  Tilt Series (Pixel Size 0.98 nm), 939.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2006-08-16-8/rawdata/S5_S474G3_110e10d22k.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6db1h-68369
title: Treponema primitia

description: Treponema primitia.  The rest of the files are in ~/Images/050811
description: <strong>Tilt Series Date:</strong> 2005-08-11</p>
<strong>Data Taken By:</strong> Gavin Murphy</p>
<strong>Species / Specimen:</strong> Treponema primitia</p>
<strong>Strain:</strong> ZAS-2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 30.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> Cells provided by Eric Matson, Jared Leadbetter; Data collected and processed by Gavin Murphy</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in standard media around exponential growth.  Removed from anaerobic tube with syringe.  Directly applied to grids, no centrifugation</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2005-08-11-1/rawdata/</p>T1_S497G2_22k12d110e.mrc,  Tilt Series (Pixel Size 0.98 nm), 1.2 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9v2e9-3t709
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-05-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-29/3dimage_69254/</p>12may04_C126_3_pnlDN_Cos7_AT5BT1_0006_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-29/3dimage_69254/12may04_C126_3_pnlDN_Cos7_AT5BT1_0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ppjmb-yhy69
title: Spiroplasma_09aug27a_grid2_010

description: Raw data files of Spiroplasma_09aug27a_grid2_010
description: <strong>Tilt Series Date:</strong> 2009-08-27</p>
<strong>Data Taken By:</strong> Morgan Beeby</p>
<strong>Species / Specimen:</strong> Spiroplasma melliferum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: 12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> grown by Bonnie data collected by Morgan processed by Bonnie</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in modified spiroplasma media with penicillin </p>
<strong>Sample Preparation:</strong> 4-minute glow discharge on Harrick glow discharger, Quantifoil R2/2 grids, 10 nm gold fiducials, "MORGAN5" Vitrobot settings</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mba2009-08-27-9/3dimage_8794/</p>09aug27a_grid2_010.mrc.rec,  Reconstruction (Pixel Size 1.9 nm), 120.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mba2009-08-27-9/3dimage_8794/09aug27a_grid2_010.mrc.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/95qyt-s9y69
title: GFP-MreB fusion methanol control

description: Raw data files of GFP-MreB fusion methanol control
description: <strong>Tilt Series Date:</strong> 2009-08-01</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, REACH</p>
<strong>Collaborators and Roles:</strong> Experiments were done by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The GFP-MreB-expressing NA1000 cells were cultured to exponential phase and treated with methanol as a control.</p>
<strong>Sample Preparation:</strong> 10 nm gold.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-77/rawdata/</p>09aug01a_methanolgfmr1_077.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-77/rawdata/09aug01a_methanolgfmr1_077.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8bgcs-rdp54
title: GFP-MreB fusion methanol control

description: Raw data files of GFP-MreB fusion methanol control
description: <strong>Tilt Series Date:</strong> 2009-08-01</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, REACH</p>
<strong>Collaborators and Roles:</strong> Experiments were done by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The GFP-MreB-expressing NA1000 cells were cultured to exponential phase and treated with methanol as a control.</p>
<strong>Sample Preparation:</strong> 10 nm gold.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-61/rawdata/</p>09aug01a_methanolgfmr1_061.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-61/rawdata/09aug01a_methanolgfmr1_061.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5wgq2-zz147
title: MreB o/e 8hr single colony

description: Raw data files of MreB o/e 8hr single colony
description: <strong>Tilt Series Date:</strong> 2010-01-09</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR and Peach</p>
<strong>Collaborators and Roles:</strong> Cells cultured and imaged by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony was picked from a PYE plate w/ 0.2 glucose and cultured o/n. The next day cells were diluted to exponential phase and induced for 8hrs w/ 0.3% xylose.</p>
<strong>Sample Preparation:</strong> R2/2 or R2/1, 10nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-01-09-43/rawdata/</p>10jan09a_MreBoe_043.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-01-09-43/rawdata/10jan09a_MreBoe_043.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0xht0-7a684
title: B. subtilis 3267

description: Raw data files of B. subtilis 3267
description: <strong>Tilt Series Date:</strong> 2011-12-07</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> AD3267, ponA null, Alp7A-GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joe Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown in LB to exponential phase</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-12-07-5/rawdata/</p>32670005.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-12-07-5/rawdata/32670005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/35kem-y2m91
title: MamJ deletion (and might contain

description: Raw data files of MamJ deletion (and might contain
description: <strong>Tilt Series Date:</strong> 2010-02-18</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum</p>
<strong>Strain:</strong> AMB-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR and Peach</p>
<strong>Collaborators and Roles:</strong> Cell culture were from Arash Komeili. Upon receiving, cells were frozen in 2 hours. Meanwhile they were kept in 30C. Data collected and processed by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Stationary phase. </p>
<strong>Sample Preparation:</strong> R2/2, 10nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-02-18-23/rawdata/</p>10feb18a_dJnofe2b_023.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.7910/dvn/tijduz
title: Behind political affiliation: how moral values, identity politics, and party loyalty have affected COVID-19 vaccination

description: The data and Stata code used to reproduce the results of the paper “Behind Political Affiliation: How Moral Values, Identity Politics, and Party Loyalty Have Affected COVID-19 Vaccination” are publicly available in the following repository. A detailed README file is provided in the Supporting Information of the original article.
DOI: 10.22002/7cheb-dwx39
title: #4-60 BaxH6 Lysogen Ecoli phage

description: Bax delta H6 bigger hole Bax mutantBaxH6 Lysogen Ecoli lamdaphageMC4100[lamdadelta (SR)]
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor failed</p>
<strong>Collaborators and Roles:</strong> Xo Luo and Xiaming Pang  xuluo@unmc.edu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> see Xiamings notes</p>
<strong>Sample Preparation:</strong> Titan 26k 7/11/13  expo 0.9 150e- poor fudicials</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-07-12-7/rawdata/</p>B1_4-600007.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/aqe85-0qb97
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-63/rawdata/</p>Caulobacter69_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 94.3 MB  
        
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        Caulobacter69.mrc.mdoc,  1.7 kB  
        
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