DOI: 10.5061/dryad.vq83bk41c
title: Data from: Molecular phylogenetics of Distephanus supports the recognition of a new tribe, Distephaneae (Asteraceae)

description: The genus Distephanus Cass. comprises 43 distinctive species of shrubs and
 small trees that have been placed historically within the ironweed tribe,
 Vernonieae (Asteraceae). Utilizing the most expansive sampling of
 Distephanus to date, this study aims to test the monophyly of this genus
 and facilitate its classification. Molecular phylogenetic analyses were
 conducted using four molecular markers from the nuclear and plastid
 genomes. These data also supported divergence dating analyses that were
 performed to understand the timing of diversification events within
 Distephanus and other related genera. Results from this study indicate
 that as currently circumscribed, Vernonieae is not monophyletic and that
 Distephanus is actually sister to a clade that comprises Vernonieae and
 another tribe, Moquinieae, which only includes two species restricted to
 Brazil. Based on these findings, Distephanus is recognized in a new tribe
 that we describe here, Distephaneae. This new tribe comprises 41 species
 of Disptehanus that are easily distinguished from Moquinieae and
 Vernonieae based on the presence of florets with yellow corollas and
 trinervate leaves.
description: Sequence data was collected from whole genomic DNA extracted from
 silica-preserved leaf tissue. Sequences were generated using Sanger
 dideoxy chain termination chemistry and chromatogram data were
 subsequently checked for accuracy using the software Geneious.
 Chromatogram data were assembled into contigs from the forward and reverse
 sequence and alignments for each locus were assembled using the software
 MAFFT. Phylogenetic reconstruction from sequence data was performed using
 the software IQ-TREE and MrBayes. A time-calibrated divergence dating
 analysis was carried out using BEAST and ancestral area reconstruction was
 carried out using BioGeoBEARS in R.  Sequence data has
 been submitted to GenBank and Accession numbers are provided in the
 Appendix that accompanies this manuscript. This Dryad submission includes
 all multiple sequence alignments, BEAST .xml configuration files, and
 BioGeoBEARS scripts (as well as the accompanying geog.txt file for
 geographic area designation). 
description: # Data accompanying Molecular phylogenetics of Distephanus supports the
 recognition of a new tribe, Distephaneae (Asteraceae)
 [https://doi.org/10.5061/dryad.vq83bk41c](https://doi.org/10.5061/dryad.vq83bk41c) ## Description of the data and file structure This data includes all multiple sequence alignments for each locus analyzed in this manuscript as well as corresponding configuration files for time-calibrated divergence dating analysis conducted using BEAST (.xml) and the script + geographic area definition file for ancestral area reconstruction analysis in BioGeoBEARS (BGB_Script_Feb2024.txt and geog.data). Each .fasta file corresponds to the multiple sequence alignment for each molecular marker used analyzed in this study, as follows: ITS_Final.fasta is a multiple sequence alignment for all sequences analyzed from the nuclear ribosomal ITS region. ndhF_Final.fasta is a multiple sequence alignment for all sequences analyzed from the plastid ndhF region. psbA_Final.fasta is a multiple sequence alignment for all sequences analyzed from the plastid psbA-trnH spacer. trnL_Final.fasta is a multiple sequence alignment for all sequences analyzed from the plastid trnL-F spacer. The BGB_Script_Feb2024.txt includes the complete script used to conduct ancestral area analyses using BioGeoBEARS and is written for the R programming language.  ## Sharing/Access information All sequence data has been submitted to GenBank and Accession Numbers are provided in the Appendix of the manuscript (which is also in the "Supplementary Information" related work of this dataset).