DOI: 10.5061/dryad.0zpc8674h
title: Climate regulates the effect of land-use change on the diversity of soil microbial functional groups and soil multifunctionality

description: Although studies have explored how soil microbial diversity and soil
 multifunctionality respond to land-use change at local scales, they have
 rarely been explored at larger scales and across different climatic and
 soil environmental conditions. By sampling 40 paired sites of land-use
 change from natural forests to agricultural lands (including croplands and
 orchards) along the middle and lower Yangtze River, combined with a global
 meta-analysis, we investigated the effects of land-use change and climate
 on the alpha and beta diversity of soil bacterial and fungal functional
 groups (FGs) and their associated soil multifunctionality at a regional
 scale. Our results showed that land-use change strongly changed the
 diversity of soil bacterial and fungal FGs and decreased
 multifunctionality, which was supported by our meta-analysis at a global
 scale. Direct effects of land-use change and climate and their
 interaction, together with changes in soil environmental variables, were
 the main determinants of the land-use change-induced changes in the
 diversity of soil bacterial or fungal FGs. The land-use change-induced
 decrease in multifunctionality was mainly associated with the direct
 effect of forest conversion, soil fertility, and diversity of fungal FGs.
 Furthermore, climate also regulated the effects of land-use change on
 multifunctionality by affecting soil fertility and fungal FGs diversity
 along the Yangtze River. Synthesis and applications. Taken together, our
 findings highlight the important effects of land-use change, climate, and
 their interactions on microbial diversity and multifunctionality, and
 suggest that effective land-use management and climate change mitigation
 strategies should be adopted to protect biodiversity and ecosystem
 function in the Yangtze River Basin.
description: # Climate regulates the effect of land-use change on the diversity of soil
 microbial functional groups and soil multifunctionality ## Data.xlsx
 Dataset from the middle and lower Yangtze River. The dataset contains 69
 variables used in this manuscript. NA in the dataset indicates missing
 data for relevant variable. | Column | Units | Description | |
 :------------- | :---------------------------------------- |
 :-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | Sample -- | -- | Unique ID for each sample | | Plot | -- | Unique ID for each plot in site | | Site | -- | Study site | | Block | -- | Unique ID for each block in site | | Land\_use | F = Natural forest; A = Agricultural land | Land use types | | S\_N | N = North; S = South | The south and north banks of the Yangtze River | | FC | 0 = Natural forest; 1 = Agricultural land | Forest conversion | | Lat | °N | Latitude for site | | Long | °E | Longitude for site | | Alt | m | Altitude for site | | MAT | °C | Mean annual temperature | | MAP | mm | Mean annual precipitation | | SM | % | Soil moisture | | Soil\_pH | -- | Soil pH | | TIN | mg kg-1 | Total inorganic nitrogen | | SOC | g kg-1 | Soil organic carbon | | TSN | g kg-1 | Total soil nitrogen | | TSP | g kg-1 | Total soil phosphorus | | Ba\_richness | -- | Richness of soil bacteria | | Ba\_beta | -- | β-diversity of soil bacteria | | BaC\_richness | -- | Richness of C-cycle bacteria | | BaC\_abundance | -- | Relative abundance of C-cycle bacteria | | BaC\_beta | -- | β-diversity of C-cycle bacteria | | BaN\_richness | -- | Richness of N-cycle bacteria | | BaN\_abundance | -- | Relative abundance of N-cycle bacteria | | BaN\_beta | -- | β-diversity of N-cycle bacteria | | Fu\_richness | -- | Richness of soil fungi | | Fu\_beta | -- | β-diversity of soil fungi | | MF\_richness | -- | Richness of mycorrhizal fungi | | MF\_abundance | -- | Relative abundance of mycorrhizal fungi | | MF\_beta | -- | β-diversity of mycorrhizal fungi | | PP\_richness | -- | Richness of plant pathogenic fungi | | PP\_abundance | -- | Relative abundance of plant pathogenic fungi | | PP\_beta | -- | β-diversity of plant pathogenic fungi | | SS\_richness | -- | Richness of soil saprotroph fungi | | SS\_abundance | -- | Relative abundance of soil saprotroph fungi | | SS\_beta | -- | β-diversity of soil saprotroph fungi | | LW\_richness | -- | Richness of soil litter-wood saprotroph fungi | | LW\_abundance | -- | Relative abundance of litter-wood saprotroph fungi | | LW\_beta | -- | β-diversity of litter-wood saprotroph fungi | | Cmin | mg kg-1 day-1 | Soil carbon mineralization | | Nmin | mg kg-1 day-1 | Soil nitrogen mineralization | | S\_NAG | μmol g-1 day-1 | Soil N-acetyl-β-D-glucosidase | | S\_GC | μmol g-1 day-1 | Solid-β-Glucosidase | | S\_LAP | μmol g-1 min-1 | Soil leucine aminopepyidase | | S\_ACP | μmol g-1 day-1 | Soil acid phosphatase | | Multi\_average | -- | Average of ecosystem multifunctionality | | Multi\_T50 | -- | 50% threshold  of ecosystem multifunctionality | | Multi\_T55 | -- | 55% threshold  of ecosystem multifunctionality | | Multi\_T60 | -- | 60% threshold  of ecosystem multifunctionality | | Multi\_T65 | -- | 65% threshold  of ecosystem multifunctionality | | Multi\_T70 | -- | 70% threshold  of ecosystem multifunctionality | | Multi\_T75 | -- | 75% threshold  of ecosystem multifunctionality | | Multi\_T80 | -- | 80% threshold  of ecosystem multifunctionality | | Geo\_PC1 | -- | Geography,  first axis of the Lat and Long | | Climate\_PC1 | -- | Climate,  first axis of the PCA on MAT and MAP | | SE\_PC1 | -- | Soil enviroment,  first axis of the PCA on SM, Soil pH and TIN | | SF\_PC1 | -- | Soil fertility,  first axis of the PCA on SOC, TSN, and TSP | | BaC\_PC1 | -- | C-cycle bacteria,  first axis of the PCA on BaC\_richness, BaC\_abundance and BaC\_beta | | BaN\_PC1 | -- | N-cycle bacteria,  first axis of the PCA on BaN\_richness, BaN\_abundance and BaN\_beta | | MF\_PC1 | -- | Mycorrhizal fungi, first axis of the PCA on MF\_richness, MF\_abundance and MF\_beta | | PP\_PC1 | -- | Plant pathogenic fungi, first axis of the PCA on PP\_richness, PP\_abundance and PP\_beta | | SS\_PC1 | -- | Soil saprotroph fungi, first axis of the PCA on SS\_richness, SS\_abundance and SS\_beta | | LW\_PC1 | -- | Litter-wood saprotroph fungi, first axis of the PCA on LW\_richness, LW\_abundance and LW\_beta | | BacF\_PC1 | -- | Bacterial functional group, first axis of the PCA on the BaC\_richness, BaC\_abundance, BaC\_beta, BaN\_richness, BaN\_abundance, BaN\_beta | | FunF\_PC1 | -- | Fungal functional group, first axis of the PCA on MF\_richness, MF\_abundance, MF\_beta, PP\_richness, PP\_abundance, PP\_beta, SS\_richness, SS\_abundance, SS\_beta, LW\_richness, LW\_abundance and LW\_beta | | Group1 | -- | Grouping in plotting | | Group2 | -- | Grouping in plotting | ## Data\_meta.csv Dataset of global meta-analysis. The dataset content 21 variables used in this manuscript. NA in the dataset indicate missing data for relevant variable. | Column | Units | Description | | :------------------ | :------------------ | :--------------------------------- | | ID | -- | Number of each data | | Title | -- | Title of the paper | | Author | -- | Author of the paper | | Study\_site | -- | Study site | | Study | -- | The number of study site | | Lat | ° | Latitude for site | | Long | ° | Longitude for site | | type\_control |  -- | Land use type of control parcel | | type\_treatment | -- | Land use type of treatment parcel | | Land\_use\_control |  F=Natural forest | Land use type of control parcel | | Land\_use\_treament | A=Agricultural land | Land use type of treatment parcel | | Xc | -- | Mean value of control group | | Sc | -- | Standard error of control group | | Nc | -- | Sample size of the control group | | Xt | -- | Mean value of treatment group | | St | -- | Standard error of treatment group | | Nt | -- | Sample size of the treatment group | | Trait | -- | Soil microbial functional groups | | yi | -- | Response ratios | | Wr | -- | Weights | ### Code/Software Any scripts,code, packages can be run using R version 4.2.1 (R Core Team, 2022). Code is uploaded as `code.txt`.