DOI: 10.5061/dryad.sj179
title: Data from: An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor

description: DNA has been used to construct a wide variety of nanoscale molecular
 devices. Inspiration for such synthetic molecular machines is frequently
 drawn from protein motors, which are naturally occurring and ubiquitous.
 However, despite the fact that rotary motors such as ATP synthase and the
 bacterial flagellar motor play extremely important roles in nature, very
 few rotary devices have been constructed using DNA. This paper describes
 an experimental study of the putative mechanism of a rotary DNA nanomotor,
 which is based on strand displacement, the phenomenon that powers many
 synthetic linear DNA motors. Unlike other examples of rotary DNA machines,
 the device described here is designed to be capable of autonomous
 operation after it is triggered. The experimental results are consistent
 with operation of the motor as expected, and future work on an enhanced
 motor design may allow rotation to be observed at the single-molecule
 level. The rotary motor concept presented here has potential applications
 in molecular processing, DNA computing, biosensing and photonics.
description: Sequential strand
 displacement in a linear construct.QCM-D data in .csv
 format. File contains overtone-normalized frequency changes for thirteenth
 overtone as a function of time, with the corresponding dissipation
 changes.Linear.csvStrand displacement in a folded nanostructure (triangle)QCM-D data in .csv format. File contains overtone-normalized frequency changes for thirteenth overtone as a function of time, with the corresponding dissipation changes. Data is provided for three cases: 1. triangle folded on surface and supplied with unfolded reverse complement of triangle. 2. pre-folded triangle supplied with unfolded reverse complement of triangle. 3. pre-folded triangle supplied with folded reverse complement of triangle.Triangle.csvThe rotary motor and its components.QCM-D data in .csv format. File contains overtone-normalized frequency changes for thirteenth overtone as a function of time, with the corresponding dissipation changes.Data corresponds to four experiments: 1. folded square A supplied with unfolded square B. 2. folded blocked square A supplied with unblocking strand and then unfolded square B. 3. folded square A supplied with folded square B. 4. full motor supplied sequentially with both unblocking strands.Motor.csvAssembly of motor componentsAgarose gel showing assembly of a surface connector, two folded squares and an unfolded square.RotaryMotorAssemblySquaresGel.jpgPolyacrylamide gel presented in Fig 4(d) of paperPolyacrylamide gel, showing the most important bands. From left to right: unfolded square of type B, folded squares of both types (B then A) and assembled motor - ‘before’ and ‘after’ operation.PAGE_Fig4d.tifAFM images acquired in response to reviewer commentsAFM images of individual folded squares and assembled motors. The structures were extraordinarily difficult to image due to their small size. Consequently they adhered poorly to the substrate and were not well-resolved.AFM.zip