DOI: 10.5061/dryad.40c31
title: Data from: The divergence and positive selection of the plant-specific BURP-containing protein family

description: BURP domain-containing proteins belong to a plant-specific protein family
 and have diverse roles in plant development and stress responses. However,
 our understanding about the genetic divergence patterns and evolutionary
 rates of these proteins remain inadequate. In this study, 15 plant genomes
 were explored to elucidate the genetic origins, divergence, and functions
 of these proteins. One hundred and twenty-five BURP protein-encoding genes
 were identified from four main plant lineages, including 13 higher plant
 species. The absence of BURP family genes in unicellular and multicellular
 algae suggests that this family (1) appeared when plants shifted from
 relatively stable aquatic environments to land, where conditions are more
 variable and stressful, and (2) is critical in the adaptation of plants to
 adverse environments. Promoter analysis revealed that several responsive
 elements to plant hormones and external environment stresses are
 concentrated in the promoter region of BURP protein-encoding genes. This
 finding confirms that these genes influence plant stress responses.
 Several segmentally and tandem-duplicated gene pairs were identified from
 eight plant species. Thus, in general, BURP domain-containing genes have
 been subject to strong positive selection, even though these genes have
 conformed to different expansion models in different species. Our study
 also detected certain critical amino acid sites that may have contributed
 to functional divergence among groups or subgroups. Unexpectedly, all of
 the critical amino acid residues of functional divergence and positive
 selection were exclusively located in the C-terminal region of the BURP
 domain. In conclusion, our results contribute novel insights into the
 genetic divergence patterns and evolutionary rates of BURP proteins.
description: Figures, Tables, and
 Additional filesFig.1 Phylogenetic
 relationships, exon-intron structure, and motif structures of BURP
 domain-containing gene. Fig. 2 Multiple sequence alignment of several BURP
 domain-containing protein sequences. Table 1. Promoter analysis of BURP
 domain-containing gene family.Table 2. Estimates of the dates of the
 segmental duplication events of the BURP domain-containing gene family.
 Table 3. Genes involved in tandem duplication and their 4DTv values. Table
 4. Functional divergence between subfamilies of the BURP domain-containing
 gene family. Table 5. Tests for positive selection among codons of BURP
 domain-containing genes using site models. Table 6 Parameter estimation
 and likelihood ratio tests for the branch-site models. Data S1 Protein
 sequence data for the BURP domain-containing gene family. Data S2 Coding
 sequence data for the BURP domain-containing gene family. Data S3 Genome
 sequence data for the BURP domain-containing gene family. Data S4 1500 bp
 of nucleotide sequences upstream of the translation initiation codon of
 BURP genes. Data S5 Multiple sequence alignment of BURP domain-containing
 gene family. Data S6. Schematic of motifs of BURP domain-containing
 proteins. Data S7. Promoter analysis performed on the BURP
 domain-containing gene family.