DOI: 10.34894/gb3z3p
title: AFM images and AFM force curves characterizing S. aureus lipid membrane upon interaction with antimicrobial peptidomimetic AMC-109, Mass Spectroscopy data of S. aureus lipid extracts
title: Experimental data for Lateral membrane organization as target of an antimicrobial peptidomimetic compound 2023

description: Atomic force microscopy (AFM) data characterizing the interaction of antimicrobial peptidomimetic AMC-109 with the lipid membrane extracted from bacteria Staphylococcus aureus. 
All experiments were done on membranes deposited on mica surface in PBS buffer at room temperature. 
AFM data were measured using JPK Nano Wizard Ultra Speed AFM. 
AFM raw images are presented in their JPK software format. 
Each AFM force-distance curve was calibrated according to a hard-response calibrating curve collected prior to each indentation of the membrane. These calibrated curves were exported in TXT format. Each TXT file contains 2 data sets corresponding to the approach and retract force-indentation curve one after another, organized in 2 columns showing "Vertical Tip Position" and "Vertical Deflection".
Mass Spectroscopy data of S. aureus lipid extracts in their raw format gathered using an Ultra-high performance liquid chromatography-mass spectroscopy instrument consisting of Accela1250 UHPLC system (Thermo Fisher Scientific) coupled to a Thermo Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with an ESO ion-source in negative ionization mode. 

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&lt;br&gt; Detailed description of the individual folders:
&lt;br&gt; Folder "Figure 1": AFM raw images of S. aureus lipid membrane before and after step by step addition of antimicrobial peptidomimetic AMC-109.

&lt;br&gt; Folder "Figure S2": AFM raw images of S. aureus lipid membrane that is expanding in time after treatment with antimicrobial peptidomimetic AMC-109.

&lt;br&gt; Folder "Figure S3": AFM raw images of S. aureus lipid membrane before and after addition of antimicrobial peptidomimetic AMC-109 in a concentration above its minimal inhibitory concentration .

&lt;br&gt; Folder "Figure S5": AFM force-indentation curves gathered on S. aureus lipid membranes without any treatment and after treatment with subcritical concentrations of the antimicrobial AMC-109. The force-indentation curves were used to characterize the mechanical properties of the membrane upon the antibiotic treatment. The folder "Figure S5" contains three subfolders that contain individual force-indentation curves measured on S. aureus lipid membranes exposed to no AMC-109 (0_ug-ml_AMC-109), 0.5 ug/ml AMC-109 (0p5_ug-ml_AMC-109), and 1 ug/ml AMC-109 (1_ug-ml_AMC-109).

&lt;br&gt; Folder "Figure S7": AFM raw images of AMC-109 layer forming on the mica support. Mica was exposed to the PBS buffer with 2 ug/ml AMC-109, this layer was scratched by contact imaging with the AFM tip. The AFM images show the recovery of the AMC-109 layer on mica.

&lt;br&gt; Folder "Figure S14": AFM raw images of the S. aureus lipid membrane before and after the exposure to 0.023 vol% of DMSO.

&lt;br&gt; Folder "Table 1 and Figure S1": Mass Spectroscopy data of S. aureus lipid extracts in their raw format.