DOI: 10.6084/m9.figshare.5812797
title: Isolation of terpenoid isolated from Sarang Semut (Myrmecodia pendans), and its performance in the inhibition and eradication of formation of Streptococcus mutans biofilm

description: This dataset contains 21 data tables and graphs relating to the determination of a terpenoid structure, isolated from Sarang Semut (<i>Myrmecodia pendans</i>) and its potential forinhibition and eradication of formation of <i>Streptococcus mutans</i> biofilm.<br>Files are in openly accessible .png image format and .xlsx format than be accessed via MS Excel or open office software.<br>Background: the related publication describes the use of compounds isolated from <i>Myrmecodia pendans</i> in preventing <i>Streptococcus mutans</i> ATCC 25175 biofilm formation, a cause of dental caries. <i>Myrmecodia pendans</i> are native plants from Papua with empirically-reported uses as natural remedies for tumors, gout, diarrhea, and fever. <br>Methodology: <i>M. pendans</i> was extracted with ethyl acetate using a Soxhlet extractor. The extract then separated and purified with the chromatography method to isolate the compound. The structure of the active compound was then characterized using the UV, IR, NMR, and MS spectrometers. The compounds obtained were induced into <i>S. mutans</i> biofilms to determine the MBIC and MBEC values.<br><b>Supporting_Data_Meirina_(1).xlsx - </b>numerical data underpinning MBEC values for <b>supporting data_meirina (12) - </b><b>supporting data_meirina (16) below</b><br><b><br></b><b>Supporting_Data_Meirina_(2).xlsx - </b>numerical data underpinning MBIC values for <b>supporting data_meirina (17) - </b><b>supporting data_meirina (18) below</b><b><br></b><br><b>Supporting_Data_Meirina_(3).xlsx - </b>numerical data involved in generating MIC values for <b>supporting data_meirina (11) below</b><br><br><b>supporting data_meirina (1).png - </b>image of Sarang senut plant (a), split in the middle (b) and cut into small pieces (c)<br><br><b>supporting data_meirina (2).png - </b><br>TLC chromatogram using G<sub>60</sub> F<sub>254</sub> silica gel plate of 1-11 fraction with <i>n</i>-hexane-ethyl-acetate (3:7, v/v) on the UV 𝛌 254 nm.<br>Separation and purification of <i>M. pendans </i>bulb ethyl acetate extract was done by TLC.<br><br>-<br><b>Files supporting data_meirina 3-10:</b><br>Determination of the structure of <b>compound 1</b> was performed by analyzing the <sup>1</sup>H-NMR,<sup>13</sup>C-NMR, HMQC, DEPT 135°, <sup>1</sup>H-<sup>1</sup>H COSY and HMBC spectrum with a 500 MHz FT-NMR spectrometer (ECA 500 JOEL variant, Japan) <br><br>The IR spectrum of the compound was determined by FT-IR Perkin Elmer Spectrum One spectrometer (Buckinghamshire, UK).<br><br>Confirmation of the conjectural structure of <b>compound 1</b> was determined using the mass spectroscopy (ES-MS Spectrometer, UPLC Type MS / MS TQD, Waters). <br><br><b>supporting data_meirina (3).png - </b>IR spectrum of Compound 1 in KBr plate<br><br><b>supporting data_meirina (4).png - </b>C-NMR Spectrum of Compound 1 (125 MHz, CD<sub>3</sub>OD)<br><br><b>supporting data_meirina (5).png - </b>DEPT 135° Spectrum of Compound 1 (125 MHz, CD<sub>3</sub>OD)<br><b><br></b><b>supporting data_meirina (6).png - </b>HMQC Spectrum showed bond between proton carbon C-21 dan C-18 of Compound 1<b><br></b><b><br></b><b>supporting data_meirina (7).png - </b>H-NMR Spectrum of Compound 1 (500 MHz, CD<sub>3</sub>OD)<b><br></b><b><br></b><b>supporting data_meirina (8).png - </b>H-HCOSY Spectrum of Compound 1 showing correlation between H-25 proton and H-26, H-27; H-28 and H-29<b><br></b><b><br></b><b>supporting data_meirina (9).png - HMBC Spectrum: </b>H-16, H-19, H-23, H-24, H-15, C-2, C-2, C-3, C-6, C-22, C-27, and C-31<b><br></b><b><br></b><b>supporting data_meirina (10).png - </b>ES-MS Spectrum of Compound 1<br><br>-<br><b>Files supporting data_meirina 11-18/Tables:</b><br><br>Determining MIC, MBIC, MBEC of Terpenoid <i>M. pendans </i>bulb towards/against <i>Streptococcus mutans</i>.<br><b>supporting data_meirina (11).png - </b>Measurement of OD 595<br><br><b>supporting data_meirina (12).png - </b>Scheme of biofilm extraction<br><br><b>supporting data_meirina (13).png - </b>Values of biofilm formed after 1 minute treatment<b><br></b><b>supporting data_meirina (14).png - </b>MBEC Value of Terpenoids of <i>M. pendens</i> bulb against <i>S. mutans</i> biofilm after 1 minute<br><b><br></b><b>supporting data_meirina (15).png - </b>Values of biofilm after 30 minutes treatment<b><br></b><br><b>supporting data_meirina (16).png - </b>MBEC Value of Terpenoids of <i>M. pendens</i> bulb against <i>S. mutans</i> biofilm after 30 minutes treatment<b><br></b><br><b>supporting data_meirina (17).png - </b>Values of biofilm formed<b><br></b><br><b>supporting data_meirina (18).png - </b>MBIC Value of Terpenoids of <i>M. pendens</i> bulb against <i>S. mutans</i> biofilm<b><br></b><br>-<br><b>supporting data_meirina (19).png - </b>The steps of isolation and purification of <i>M. pendens</i> bulb<br><br><b>Figures 1 and 2 from the associated publication:</b><br><b>fig 1.png - </b>TLC chromatogram using ODS plate fraction 3-(7-9)-(7&8) with 100% eluent methanol on the UV light at λ 254 nm (a); UV light at λ 365 nm (b); and after sprayed with stain-visible agent 10% H2SO4 solved in ethanol (c).<br><b>fig 2.png - </b>Conjectural structure of compound 1<br>For a full descriptionof acronyms and methods see the associated publication.<br>