DOI: 10.5061/dryad.zcrjdfnsm
title: Disrupted development of the mPFC-thalamic circuit in Shank3–/– mice, an autism-associated model

description: This dataset contains the complete quantitative data used to generate all
 figures presented in the associated manuscript. Data are organized in a
 single Excel file, with each sheet corresponding to a specific figure
 panel. Each entry represents an individual cell, slice, or animal,
 depending on the experiment, and includes relevant metadata such as
 genotype and experimental parameters. Columns specify measured variables
 (e.g., electrophysiological properties, behavioral metrics, or response
 amplitudes), some summary statistics, and normalized values when
 applicable. The file also includes a list of the raw data filenames used
 for figure generation, facilitating transparency and reproducibility. All
 data were collected under standardized acquisition and analysis pipelines
 to ensure internal consistency.
description: # Disrupted development of the mPFC-thalamic circuit in Shank3–/– mice, an
 autism-associated model Dataset DOI:
 [10.5061/dryad.zcrjdfnsm](https://doi.org/10.5061/dryad.zcrjdfnsm) ##
 Description of the data and file structure This dataset contains all the
 data used to generate the figures and supplementary figures in the above
 manuscript. The study investigates developmental and adult alterations of
 the **medial prefrontal cortex (mPFC)–thalamic circuit** in **Shank3–/–
 mice**, a model of autism spectrum disorder. The dataset is organized into
 multiple Excel tabs, where each tab corresponds to a **specific figure or
 supplementary figure**. Both **male and female** mice are included, and
 statistical tests (including normality testing and resulting p-values) are
 provided within the file.  For any queries regarding the data, please
 contact the author, Gabrielle Devienne [devienne@stanford.edu] ### Files
 and variables #### File: Devienne_2025_Manuscript_DataPerFigures.xlsx
 **Description:** Excel file with tabs per figure, figure panels, or
 supplementary figure data and metadata information Each figure tab
 includes a table with the following metadata (when applicable): * **Animal
 ID** * **Cage number** * **Sex** * **Date of Birth (DOB)** * **Date of
 Experiment (DOE)** * **Genotype** (WT or Shank3) * **Raw data file name**
 (from which experimental data were derived) #### File Organization All
 data are provided in a single Excel workbook, where: * Each **main
 figure** corresponds to one primary tab. * Associated **supplementary
 figures**. * Raw file names are included to link each row to the original
 acquisition file. #### Content Summary by Figure **Figure 1, Figure1_USV,
 Supplementary Figure 1 — Behavioral Data** **Ultrasonic vocalizations
 (USVs)** recorded at **P11** using *Avisoft Bioacoustics*.\ Parameters
 include: * Call duration, interval, break, DistToMax *
 Start/End/Centre/Max peak frequency, amplitude, and entropy * Real-time
 recording timestamp * Reject flag (for Matlab plotting/analysis purposes) 
 Supplementary Figure 1 contains call-type categorization and statistics.
 **Adult social interaction** behavior analyzed using *ezTrack* is also
 included. **Figure 2 & Supplementary Figure 2 — CSD Analysis** Current
 source density (CSD) measurements from **mPFC slices** recorded with **TDT
 hardware** and analyzed in **MATLAB**.\ Values represent peak amplitude
 (µA/mm³) for: * L2/3 presynaptic sink * L2/3 postsynaptic source * L5
 presynaptic source * L5 postsynaptic sink Electrical stimulation
 intensities range from **100–800 µA**. **Figure 3 & Supplementary
 Figure 4/7 — Patch-Clamp Electrophysiology (L2/3 and L5 Pyramidal
 Neurons)** Includes metadata (genotype, age, intracellular/extracellular
 solution), action potential properties, firing responses to depolarizing
 steps (–100 to +400 pA), and **excitatory/inhibitory (E/I) ratios** based
 on EPSCs at –70 mV and IPSCs at +10 mV. Supplementary Figure 3 provides
 additional intrinsic properties: rheobase, AHP, threshold, AP width, AP
 overshoot, sag, and rebound. * **Sholl morphology analysis**
 (Supplementary Figure 4) * **Supplementary Figure 7:** E/I measurements
 using cesium-based internal solution (L2/3 and L5). **Figure 4 &
 Supplementary Figure 8 — Patch-Clamp in mediodorsal thalamus
 (MD)-Projecting mPFC Neurons** Adult mPFC neurons retrogradely labeled
 with **retrobeads** projecting to the **MD thalamus**.\ Includes: * Cell
 metadata (RMP, Ri, rheobase, acquisition rate) * Spiking profiles *
 **Supplementary Figure 8:** Action potential properties for cells recorded
 in Figure 4. **Figure 5 & Supplementary Figure 9 — MD Neuron Responses
 to Optogenetic Stimulation** Whole-cell voltage-clamp of **MD thalamic
 neurons** stimulated via **mPFC ChR2-expressing axons** at: * **1 Hz** *
 **10 Hz (10-pulse train)** **Supplementary Figure 9** contains additional
 1 Hz current traces. **Figure 6 — Persistent Current Analysis** Persistent
 currents during 10 Hz trains in adult WT and Shank3–/– mice under: *
 **AP5** (NMDA receptor antagonist) * **DHPG** (mGluR1/5 agonist)
 **Software & Acquisition Tools** * **Avisoft Bioacoustics** (USV
 recording and analysis) * **ezTrack** (behavior
 analysis) ([https://github.com/denisecailab/ezTrack](https://github.com/denisecailab/ezTrack)) * **TDT system** (slice electrophysiology acquisition, LFP/CSD) * **MATLAB** (CSD and electrophysiology data processing) * **GraphPad Prism** for analysis and statistics * **ImageJ / SNT plugin** (morphology analysis) * **pClamp / Axon Instruments** (patch clamp acquisition