cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.57903/uj/ofl8dm
title: In one rhythm. Attachment styles and physiological and behavioral synchronization of participants attend in group psychological training conducted face to face and online - questionnaire data
title: W jednym rytmie. Style przywiązania a synchronizacja fizjologiczna i behawioralna uczestników grupowego treningu psychologicznego prowadzonego stacjonarnie i online - dane kwestionariuszowe

description: Dane pozyskane w ramach projektu badawczego "W jednym rytmie. Style przywiązania a synchronizacja fizjologiczna i behawioralna uczestników grupowego treningu psychologicznego prowadzonego stacjonarnie i online”. Projekt badawczy został sfinansowany ze środków Narodowego Centrum Nauki (2022/06/X/HS6/00621). W ramach badań zebrano dane kwestionariuszowe: podczas pierwszej i ostatniej (ósmej) sesji badawczej, a także po każdym spotkaniu. Kwestionariusze: ECR (Experience in Close Relationship); KDG – Kwestionariusz Doświadczenia Grupowego, GCQ – Group Climate Questionnaire , SES – Rosenberg Self-Esteem Scale; TIPI - Ten Item Personality Questionnaire, GRQ - Group Readiness, TFI - Terapeutic Factor Inventory, Kwestionariusz danych demograficznych
description: Data obtained as part of the research project "In one rhythm. Attachment styles and physiological and behavioral synchronization of participants of group psychological training conducted on-site and online. The research project was financed by the National Science Center (2022/06/X/HS6/00621). As part of the research, questionnaire data were collected: during the first and last (eighth) research session, as well as after each meeting. Questionnaires: ECR (Experience in Close Relationship); KDG – Group Expierience Questionnaire, GCQ – Group Climate Questionnaire , SES – Rosenberg Self-Esteem Scale; TIPI - Ten Item Personality Questionnaire, GRQ - Group Readiness, TFI - Terapeutic Factor Inventory, Demographic Questionnaire
DOI: 10.6075/j0cj8cpx
title: CIL:1196, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
DOI: 10.6075/j0w094n3
title: CIL:11959, Chaetopterus sp. 1 Woods Hole MA, oocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: See DA Lutz et al 1988. Micromanipulation of the asymmetric positioning of the maturation spindle in Chaetopterus sp oocytes. Cell Motil Cytoskel 11:83-96. This paper includes stills of similar experiments.
description: This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. The spindle and its orientation are made visible by the use of polarizing optics, and in this sequence, rotation of the spindle causes a temporary reversal of its contrast from bright to dark. When the needle is withdrawn, the spindle returns to its original position, but the opposite pole is now attached to the cortex.
DOI: 10.6075/j06t0kb3
title: CIL:11958, Chaetopterus sp. 1 Woods Hole MA, oocyte. In Cell Image Library

description: This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. This movie, speeded up about 10-fold, shows the transient puckering in the cell membrane showing that the spindle pole is attached to the cortex. After the attachment has been broken, the cell surface rounds back up. The spindle is made visible by the use of polarizing optics.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: See DA Lutz et al 1988. Micromanipulation of the asymmetric positioning of the maturation spindle in Chaetopterus sp oocytes. Cell Motil Cytoskel 11:83-96. This paper includes stills of similar experiments.
DOI: 10.6075/j0kd1wpz
title: CIL:11961, Chaetopterus sp. 1 Woods Hole MA, oocyte. In Cell Image Library

description: See DA Lutz et al 1988. Micromanipulation of the asymmetric positioning of the maturation spindle in Chaetopterus sp oocytes. Cell Motil Cytoskel 11:83-96. This paper includes stills of similar experiments.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. This movie, speeded up about 10-fold, illustrates the positional 'memory' of the system. After the spindle has been moved to the center of the cell, it returns to its original position and orientation. The spindle and its orientation are made visible by the use of polarizing optics.
DOI: 10.6075/j018356d
title: CIL:11962, Phallusia mammilata, egg. In Cell Image Library

description: The time lapse movie spans fertilization, ooplasmic segregation, and early divisions in the egg of the tunicate Phalusia mammilata. Contrast is provided by both polarizing microscopy and differential interference microscopy.
The entrance of the sperm (upper right) stimulates waves of contraction in the cytoplasm. Polar bodies are formed and extruded, after which male and female pronuclei migrate toward each other, and nuclear fusion occurs. Finally, the zygote proceeds through mitosis and cleavage stages.
description: See: Sardet et al. (1989) Fertilization and ooplasmic movements in the ascidian egg. Development 105:237-249.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy; differential interference contrast microscopy
Parameter imaged: retardance; optical path length gradient
Source of contrast: form birefringence; boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Data qualification: Processed
DOI: 10.6075/j05h7f1n
title: CIL:11991, Drosophila melanogaster, epithelial cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: microtubule polymerization; mCherryFP
Processing history: unprocessed raw data; color combine
Data qualification: Raw
description: TIRF time-lapse imaging of EB1-GFP (green) and mCherry-tubulin (red). EB1-GFP is seen on microtubule tips that grow to the cortex but does not accumulate on stable microtubule bundles at the equator, in contrast to kinesin-6 . Corresponds to video 8 from J Cell Bio 186:727-738, 2009.
Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org).
DOI: 10.6075/j0p849jt
title: CIL:11960, Chaetopterus sp. 1 Woods Hole MA, oocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. This movie, speeded up about 10-fold, illustrates the ability of the spindle to return to its attachment site at the cell cortex after being moved. The spindle is made visible by the use of polarizing optics.
description: See DA Lutz et al 1988. Micromanipulation of the asymmetric positioning of the maturation spindle in Chaetopterus sp oocytes. Cell Motil Cytoskel 11:83-96. This paper includes stills of similar experiments.
DOI: 10.6075/j0zw1kxm
title: CIL:11995, Danio rerio, mucus secreting cell, immune cell. In Cell Image Library

description: Preparation: living tissue
Item type: fluorescence microscopy
Imaging mode: living tissue
description: A polymorphic immune cell (red) glides over two spherical V12Ras-transformed mucus-secreting cells (green, possible tumor precursors) in a live three day old zebrafish embryo. This is one of four images in a series that illustrates the movement of the immune cell over the surface of the transformed mucus-secreting cells.
DOI: 10.6075/j08w3c11
title: CIL:11992, Drosophila melanogaster, epithelial cell. In Cell Image Library

description: TIRF time lapse of mCherry-tubulin (left), myosin-GFP (middle), and overlay (myosin in green and tubulin in red; right) in a cell with a monopolar spindle from metaphase to anaphase. The S2 cells are treated with dsRNA to Klp61F (kinesin 5 motor) and BubR1 (checkpoint protein)). This video corresponds to the image in Fig. 5 A and video 9 from J Cell Bio 186:727-738, 2009.
Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org).
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data; color combine
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j03n2249
title: CIL:11990, Drosophila melanogaster, epithelial cell. In Cell Image Library

description: Time lapse of Aurora-B GFP and mCherry microtubules at the metaphase-to-anaphase transition. The laser illumination was set near to but not achieving complete total internal reflection TIRF to allow visualization of microtubules at the cortex as well as the more interior central spindle. Aurora B foci are seen at the tips of microtubules at multiple sites at the cortex as well as a bright central region (the central spindle).This video corresponds to the image in Fig. S4 and video 6 from J Cell Bio 186:727-738, 2009.
Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2 -3 s with a 50 -200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org).
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data; color combine
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0n29w5f
title: CIL:1199, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j07080mq
title: CIL:12025, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm
description: Movie of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. 
Time-lapse polarization microscopy using a Nikon 
Microphot SA, equipped for liquid crystal polarized light microscopy 
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30/60/90 sec for 2 hr 31 min using a QImaging Retigo EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for the movie is included in this grouped set.
DOI: 10.6075/j01c1vjf
title: CIL:12011, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy; speckle microscopy
Parameter imaged: elastic scattering of photons; fluorescence emission
Source of contrast: differences in amount of elastic light scattering; distribution of a specific protein
Visualization methods: Alexa Fluor 546; phalloidin; visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Visualization of F-actin network movement in motile keratocytes with FSM. F-actin flows slowly from the leading edge to the cell body in motile cells. Video is paired phase-contrast and AF546-phalloidin FSM time-lapse images. 30x real time (frames were collected at 2-s intervals and are displayed at 15 frames/s). Phalloidin was conjugated to AlexaFluor546 for live cell FSM imaging. Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Images were acquired using an inverted microscope (Diaphot-300; Nikon) with a 60× NA 1.4 oil plan-Apo objective (Nikon) every 2 with the 60× oil objective. All time-lapse images were collected with a cooled back-thinned CCD camera (MicroMax 512BFT; Princeton Instruments) with a 2× optovar attached using MetaMorph software version 6 (Molecular Devices). Video corresponds to Fig. 1 and video 2 in JCB 178:1207-1221, 2007.
DOI: 10.6075/j0s75f3m
title: CIL:12010, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library

description: Visualization of F-actin network movement in stationary keratocytes with FSM. F-actin flows centripetally from the cell periphery to the cell body in stationary cells. Video shows paired phase-contrast and AF546-phalloidin FSM time-lapse images. Phalloidin was conjugated to AlexaFluor546 for live cell FSM. Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Images were acquired using an inverted microscope (Diaphot-300; Nikon) with a 60× NA 1.4 oil plan-Apo objective (Nikon). Images were acquired every 3 s with a 60× oil objective and a cooled back-thinned CCD camera (MicroMax 512BFT; Princeton Instruments) with a 2× optovar attached using MetaMorph software version 6 (Molecular Devices). 30x real time (frames were collected at 3-s intervals and are displayed at 10 frames/s). Corresponds to Fig. 1 C and Video 1 of JCB 178:1207-1221, 2007.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy; speckle microscopy
Parameter imaged: elastic scattering of photons; fluorescence emission
Source of contrast: differences in amount of elastic light scattering; distribution of a specific protein
Visualization methods: Alexa Fluor 546; phalloidin; visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0t153pb
title: CIL:12009, Danio rerio, mucus secreting cell, immune cell. In Cell Image Library

description: A polymorphic immune cell (red) glides over two spherical V12Ras-transformed mucus-secreting cells (green, possible tumor precursors) in a live three day old zebrafish embryo. This is one of four images in a series that illustrates the movement of the immune cell over the surface of the transformed mucus-secreting cells.
description: Preparation: living tissue
Item type: fluorescence microscopy
Imaging mode: living tissue
DOI: 10.6075/j0gm861z
title: CIL:11989, Drosophila melanogaster, epithelial cell. In Cell Image Library

description: TIRF time-lapse imaging of myosin-GFP (left) and mCherry-tubulin (right) in an S2 cell depleted of kinesin-6 (Pav) by RNAi. Myosin-GFP localization to the equator and loss from the poles does not occur after RNAi kinesin-6; microtubules tend to be more homogeneous throughout the cortex and less bundled.
Video corresponds to Fig 3B and video 6 from J Cell Bio 186:727-738, 2009. Note that the wild type control is available as another video in the series. 

Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org).
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements