cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0sb44gg
title: CIL:12202, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2A panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0jw8cm1
title: CIL:12193, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2A panels 13-16 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j00k278j
title: CIL:12183, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of CENP-E(green) in spindle midzone during mitosis in INCENP OFF cells expressing INCENP F802A (red). DAPI (DNA) is blue. See Fig 8E panels 5-8 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0t152bf
title: CIL:12185, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) in outer kinetochores during mitosis in INCENP OFF cells expressing WT TrAP-INCENP (red) in the presence of ZM447439. DAPI (DNA) is blue. See Fig 8F panels 1-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0cr5s2r
title: CIL:12189, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) at kinetochores during mitosis in INCENP ON cells. Alpha-tubulin is red and DAPI (DNA) is blue. See Fig 8A panels 1-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j00v8bhz
title: CIL:12188, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of CENP-E(green) to spindle midzone during mitosis in INCENP ON cells. Alpha-tubulin is red and DAPI (DNA) is blue. See Fig 8A panels 5-8 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0jh3jt3
title: CIL:12184, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of CENP-E(green) in spindle midzone during mitosis in INCENP OFF cells expressing WT TrAP-INCENP (red) in the presence of ZM447439. DAPI (DNA) is blue. See Fig 8F panels 5-8 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0n0157x
title: CIL:12178, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B (green) and DNA (blue) in an INCENP-OFF cell expressing TrAP-INCENP WT, INCENP and Aurora B transfer normally to spindle midzone. See Fig 6A panel 5 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0f18xhr
title: CIL:12176, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP OFF cells expressing mutant TrAP-INCENP W766G See Fig 6B panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0pg1qfc
title: CIL:12175, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP-OFF cells expressing mutant TrAP-INCENPW766G. See Fig 6b panel 9 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0mc8xrw
title: CIL:12186, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) to outer kinetochores during mitosis in INCENP OFF cells expressing TrAP-INCENP F802A (red). DAPI (DNA) is blue. See Fig 8E panels 1-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0st7nh3
title: CIL:12180, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of CENP-E(green) in spindle midzone during mitosis in INCENP OFF cells. Alpha-tubulin is red and DAPI (DNA) is blue. See Fig 8B panels 5-8 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j07w69w7
title: CIL:12179, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B (green) and DNA (blue) in INCENPOFF cells expressing TrAP-INCENP WT. See Fig 6A panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0pn94c6
title: CIL:12192, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2A panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0v123h8
title: CIL:12191, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of CENP-E(green) to spindle midzone during mitosis in INCENP OFF cells expressing TrAP-INCENP WT (red). (DNA) is blue. See Fig 8C panels 5-8 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j01v5cps
title: CIL:12190, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) to kinetochores during mitosis in INCENP OFF cells. Alpha-tubulin is red and DAPI (DNA) is blue. See Fig 8B panels 51-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0hd7td6
title: CIL:12174, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B (green) and DNA (blue) in INCENPOFF cells expressing mutant TrAP-INCENP-F802A. See Fig 6C panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j0fq9vbz
title: CIL:12173, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP-OFF cells expressing mutant TrAP-INCENPF802A,. See Fig 6C panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
DOI: 10.6075/j0pn94br
title: CIL:12171, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed
description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. INCENP localizes to centromeres in prometaphase, but fails to transfer to the spindle at anaphase in wild type clone 18 cells grown in the Aurora B inhibitor ZM447439. Cells were stained for INCENP (green), plus alpha-tubulin (red) and DAPI for DNA (blue). See Fig 6D panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.
DOI: 10.6075/j00k27x2
title: CIL:41130, Enterobacteria phage T4. In Cell Image Library

description: Preparation: whole mounted tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Images collected on a JEOL 1010 TEM. Please refer to http://remf.dartmouth.edu:8080/EM-Wiki for details on specimen preparation.
description: Transmission electron microscope image of T4Phage, a bacteriophage that infects Escherichia coli bacteria.This image is part of an image group (CIL:41124-41131).
DOI: 10.6075/j07h1hxg
title: CIL:41129, Enterobacteria phage T4. In Cell Image Library

description: Preparation: whole mounted tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Transmission electron microscope image of T4Phage, a bacteriophage that infects Escherichia coli bacteria.This image is part of an image group (CIL:41124-41131).
description: Images collected on a JEOL 1010 TEM. Please refer to http://remf.dartmouth.edu:8080/EM-Wiki for details on specimen preparation.
DOI: 10.6075/j0x34wvb
title: CIL:41127, Enterobacteria phage T4. In Cell Image Library

description: Transmission electron microscope image of T4Phage, a bacteriophage that infects Escherichia coli bacteria.This image is part of an image group (CIL:41124-41131).
description: Preparation: whole mounted tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
description: Images collected on a JEOL 1010 TEM. Please refer to http://remf.dartmouth.edu:8080/EM-Wiki for details on specimen preparation.
DOI: 10.6075/j02j69q0
title: CIL:40419, Saccharomyces cerevisiae, arf1∆ mutant. In Cell Image Library

description: Transmission electron micrograph of crescent-shaped organelles from an arf1‚àÜ mutant cell treated with 60¬µM sertraline. This image is a high-magnification zoom of the cell in CIL 40411 (25,000X).This image is part of an image group of cisternae images, CIL: 40417-40423. The contributor invites Library users to comment on whether these organelles are related to the endoplasmic reticulum.
description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.