DOI: 10.5061/dryad.2z34tmpxm
title: Data from: Dynamics of phosphorus pools in drainage ditch sediments within an agricultural catchment

description: Hydrological dynamics in drainage ditch sediments may impact phosphorus
 (P) retention and its potential (re-)release to connected waters. We aimed
 at understanding the fate of P in drainage-ditch sediments under dynamic
 environmental conditions (i.e., drying-rewetting cycles) by examining P
 pool transformations through an isotopic labelling and incubation
 experiment. Fresh sediment samples of a drainage ditch were labeled with
 18O-enriched inorganic P (Pi), followed by a twenty-four-week incubation
 under different environmental conditions. A five-step sequential
 extraction scheme was combined with δ18O analysis of Pi (δ18O Pi) to
 follow changes in the operationally defined P pools. Few P pools showed
 significant changes, but time-resolved results revealed a dynamic
 interplay between redox conditions, microbial activity, and P dynamics. A
 subsequent decline in microbial P concentrations suggested cell death and
 reduced microbial activity, likely caused by hydrological stress. The
 δ18OPi values in surface-adsorbed pools retained traces of the original Pi
 label after twenty-four weeks, allowing tracking of long-term P
 transformations and distinguishing biological and geochemical reactions.
description: # Data from: Dynamics of phosphorus pools in drainage-ditch sediments
 within an agricultural catchment. ### Authors: Christiane Nagel, Harald
 Neidhardt, Yvonne Oelmann Description: This dataset represents the results
 of an isotope-labelling and laboratory incubation experiment aiming to
 reveal dynamics of phosphorus pools in drainage-ditch sediments. The data
 were checked for normal distribution (Shapiro-Wilk test, p &gt; 0.05) and
 homogeneity of variances (Levene´s test; p &gt; 0.05). Repeated Measures
 ANOVA was used to test differences within a treatment across the different
 time points (significance level: 0.05). One-way ANOVA was used to test
 differences between the treatments for each of the dependent variables (P
 pools, Pmic, APA, Δ18OPi). A non-parametric LSD post-hoc test (Least
 Significant Difference) was applied if required. To determine mean
 differences between two pairs or time points, a paired t-test was used.
 ### Files: Data_DrainageDitch_2021_csv.csv Data folder: Includes five
 spreadsheets with general information and the data measured in the
 incubation experiment. Note: N/A means not applicable or missing data. 1.
 Experimental overview: Includes description of the experimental setup and
 data analysis consideration. 2. Parameter info: Includes information of
 acronyms used, measured data with calculations and devices used and
 related references. 3. Processed Data: Includes calculated data from raw
 data with minimum, maximum, mean and standard deviation. 4. Raw data:
 Includes all values of the real triplicates measured during the
 experiment. 5. Temperature and Redox data: Includes Temperature and Redox
 data from electrodes measured during the experiment. ### Code/Software:
 Statistical analyses were conducted in Excel (version 1808, 2019 MSO) and
 SPSS (IBM SPSS Statis-tics, Version 29).