cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14305345
title: Correlation of maternal concentrations of plasma testosterone with fetal sex in horses

description: ABSTRACT: The objectives of this study were to evaluate the correlation of fetal sex and plasma testosterone concentrations between the 5th and 8th months of pregnancy in mares and to verify the applicability of this test to predict fetal sex. Blood samples were collected from 21 mares at 30-day intervals of between 150 and 240 days of pregnancy. Plasma testosterone was determined by radioimmunoassay and the sex of the foals confirmed at birth. The levels of maternal testosterone were higher in mares carrying female fetuses at months 5 and 8 (P &lt; 0.05). Limit values were determined by analyzing the receiver operating characteristic (ROC) estimates: 35.5 pg/mL and 40 pg/mL for the 5th and 8th month, respectively. For the mares with plasma testosterone values equal to or above the threshold, gestation of female foals was predicted, and for those with plasma testosterone below the threshold values pregnancy of male foals was predicted. In the 5th month, the predictive values for male and female fetuses were 70% and 88.9%, respectively; the detection rates were 87.5% and 72.7%, and the total accuracy of the examination was 78.9%. In the 8th month, the predictive values for male and female fetuses were 80% and 90%, respectively; the detection rates were 88.9% and 81.8%, and the total accuracy of the examination was 85%. It was concluded that there was a correlation between fetal sex and plasma testosterone concentrations in pregnant mares. Prediction of fetal sex based on plasma concentrations of maternal testosterone can be performed in months 5 and 8 with 78.9% and 85% accuracy, respectively.
DOI: 10.6084/m9.figshare.14305344
title: Diagrammatic scale for assessment of anthracnose severity in feijoa fruit

description: ABSTRACT: A diagrammatic scale of anthracnose in feijoa fruit was elaborated and validated in order to standardize disease severity assessments. The proposed scale showed six disease severity levels: 2, 10, 20, 40, 70 and 100% of the injured fruit surface. The scale took into account the minimum and maximum limits of disease severity observed in the field and the intermediate values followed logarithmic increments according to the Weber-Fechner stimulus-response law. Eight inexperienced raters validated the scale by quantifying the disease severity (using/not using the scale) of 50 feijoas with anthracnose symptoms. In conclusion, the scale improved the assessment of anthracnose in feijoa. Eight genotypes from different crosses were tolerant to anthracnose.
DOI: 10.6084/m9.figshare.14305343
title: Use of Saccharomyces cerevisiae-based products and effects on rumen environment and performance of sheep subjected to dietary changes

description: ABSTRACT: Natural additives in ruminant diet optimizes animal performance by controlling or modifying the rumen fermentation pattern. The aim of this study was to evaluate the effects of using Saccharomyces cerevisiae products during abrupt changes in dietary starch concentration on ruminal pH maintenance, performance and metabolism of confined sheep. Twenty adult ewes, crossbred Texel x Corriedale(44.7±6.97 Kg BW) were divided into three groups: Control (n=6), Cultron X (yeast culture; n=7) and Cultron Pro (hydrolyzed yeast; n=7), administered at a dose of 5 g/sheep/day (10x1010CFU/g DM). The experiment lasted 20 days divided into four periods, alternating between 40 and 60% concentrate. Blood and ruminal fluid were collected at the end of each period, and daily dry matter intake was measured to determine the average daily gain (ADG). Blood tests included blood count and biochemical parameters such as glucose, urea, total plasma proteins (TPP), liver enzymes, acute phase proteins and minerals. There was an upward trend in ADG in group Cultron X compared to control in period 3. Although, ruminal pH did not differ between groups, protozoan motility was higher in animals supplemented with hydrolyzed yeast. The Cultron Pro group showed an increase in TPP in periods 3 and 4, and AST in periods 2 and 3 compared to the control group. There was no difference in other metabolites. Yeast supplementation is a potential tool in times of feeding challenge, in which yeast culture is the most effective for improving performance of confined sheep being in improving performance of confined sheep.
DOI: 10.6084/m9.figshare.14305340
title: Nisin and ε-poly-L-lysine as natural antimicrobials towards spoilage-associated Lactobacillus plantarum

description: ABSTRACT: Sausages are highly susceptible to microbial spoilage. Lactic acid bacteria (LAB) is the main group of spoilage bacteria in vacuum packed cooked sausages. To control microbial growth natural antimicrobials have been used as food preservatives. The aim of this study was to identify strains of lactic acid bacteria isolated from spoiled commercial Calabresa sausages and use them in an in vitro challenge with the natural antimicrobials, nisin (NI) and ε-poly-L-lysine (ε-PL). Mass spectrometry identification of LAB isolated from sausages using MALDI-TOF revealed a predominance of L. plantarum in the LAB population. RAPD-PCR of L. plantarum strains showed four different genetic profiles. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of NI and ε-PL, alone and in combination, against a pool of different profiles L. plantarum were determined. MIC of NI and ε-PL were 0.468 mg/ L and 75 mg/ L; respectively, whereas MBC of NI and ε-PL were 12.48 mg/L and 150 mg/L, respectively. The combined effect of NI and ε-PL was determined using concentrations at 1/4 and 1/8 of individual MICs. Synergistic effect was confirmed at both concentrations showing a fractional inhibitory concentration index of 0.5 and 0.2, respectively. The combination of NI and ε-PL at a small concentration of 0.05 mg/L and 9.375 mg/L, respectively, showed inhibitory effect towards spoilage L. plantarum Results show the potential of the combined use of NI and ε-PL to control sausage spoilage-associated with lactobacilli.
DOI: 10.6084/m9.figshare.14305337
title: Focal myositis associated with Leishmania spp. infection in a dog - diagnostic approach

description: ABSTRACT: Canine visceral leishmaniasis is a systemic, zoonotic disease widely spread in several countries. The disease is caused by Leishmania spp., and the dog is the main reservoir of this parasite. Clinical signs in the muscle skeletal system consist of muscle atrophy, weakness, lameness, abnormal locomotion, osteitis, polyarthritis, heat and swelling of the joints, enlarged local lymph nodes and pain. In this note, a case of canine myositis of the lumbar region associated with Leishmania spp. infection is reported. Clinical signs included weakness, fever, mild dehydration, enlarged mandibular, pre-scapular and popliteal lymph nodes and a large palpable soft mass in the lumbar region, semi-adhered and not painful. Serologic diagnosis resulted reagent by indirect immunofluorescence reaction method. Findings of ultrasonography of the lower back are described, revealing the misalignment of muscle fibers, interspersed with anechoic areas compatible with edema. Local fine needle aspiration cytology was crucial for a definitive diagnosis, revealing amastigote forms. In endemic areas of leishmaniasis, clinicians should consider this disease as a differential diagnosis in the presence of musculoskeletal injuries with no apparent cause.
DOI: 10.6084/m9.figshare.14305336
title: Formulation of fishburgers with the addition of different protein sources and taro flour

description: ABSTRACT: The present study aims to develop Nile tilapia hamburger with incorporation of whey, collagen from chicken feet and taro flour. A complete factorial design 2³ was performed to investigate the influence of the variables on weight loss, shrinkage, color and shear force, that were used to propose mathematical models. Proximate composition, microbiological characteristics and sensory properties were also evaluated. Factors such as taro flour and whey were significant and contributed to a higher yield for fish burgers, with negative effects on shrinkage and weight loss. The fishburgers had a soft texture, although collagen and its interaction with taro flour contributed to the increase in shear force. The factors had influence only on the b* value with yellowish hue. The protein and ash values obtained were higher than those of the control formulation. The sensory characteristics of the hamburgers were not affected by the factors, being all classified with high acceptability. The formulation represented by the central point (F9) containing 3.0% of taro flour, 0.5% chicken feet collagen and 3.0% powdered whey, obtained greater purchase intention. Therefore, the addition of taro flour, collagen from chicken feet and whey is an alternative in the use of industrial by-products to add value to fish burgers with market potential.
DOI: 10.6084/m9.figshare.14305334
title: Symbiotic drink based on Brazil nuts (Bertholletia excelsa H.B.K): production, characterization, probiotic viability and sensory acceptance

description: ABSTRACT: Brazil nut is a typical oilseed from the Amazon region, of great economic and cultural importance. Due to its high nutritional value, it is interesting to be used as a matrix for new products’ development. Thus, the present study aimed to develop and characterize a drink based on Brazil nuts fermented by Lactobacillus casei and added with inulin. After formulated, the drink was pasteurized, fermented, maturated, and stored under refrigeration for 28 days. Physical, chemical, microbiological, and sensory analyzes were performed before and after fermentation, at 7-day intervals. There was maintenance in the content of lipids and proteins and reduction in the concentrations of fibers, ash, total carbohydrates, and moisture. The fermented drink pH decreased significantly during storage, while the acidity and the soluble solids content increased from the 14th day. Regarding the color, there were significant changes in all parameters analyzed. In the fermented drink, the development of molds and yeasts, total and thermotolerant coliforms, and Salmonella were not verified, while the control drink deteriorated after 7 days. The probiotic L. casei had count ranging from 9.48 to 8.59 log CFU mL-1 during the 28 days. When exposed to in vitro gastrointestinal conditions, L. casei significantly reduced its concentration. However, it reached the enteric phase with counts greater than 6.00 log UFC mL-1. Sensorially, the drink reached intermediate scores (between 5.41 and 6.02). Therefore, the use of Brazil nuts proved to be viable for the development of a product free of animal-origin components, symbiotic, and of outstanding nutritional quality.
DOI: 10.6084/m9.figshare.14305333
title: Choice of non-linear models to determine the growth curve of meat-type quail

description: ABSTRACT: The objective of this study was to compare non-linear models fitted to the growth curves of quail to determine which model best describes their growth and check the similarity between models by analyzing parameter estimates.Weight and age data of meat-type European quail (Coturnix coturnix coturnix) of three lines were used, from an experiment in a 2 × 4 factorial arrangement in a completely randomized design, consisting of two metabolizable energy levels, four crude protein levels and six replicates. The non-linear Brody, Von Bertalanffy, Richards, Logistic and Gompertz models were used. To choose the best model, the Adjusted Coefficient of Determination, Convergence Rate, Residual Mean Square, Durbin-Watson Test, Akaike Information Criterion and Bayesian Information Criterion were applied as goodness-of-fit indicators. Cluster analysis was performed to check the similarity between models based on the mean parameter estimates. Among the studied models, Richards’ was the most suitable to describe the growth curves. The Logistic and Richards models were considered similar in the analysis with no distinction of lines as well as in the analyses of Lines 1, 2 and 3.
DOI: 10.6084/m9.figshare.14305331
title: Image processing to identify damage to soybean seeds

description: ABSTRACT: In Brazil and worldwide, commercialization of soybeans is of great importance to the economy, making their quality considered. The presence of damaged soybean seeds decreases the added value of the product. Businesses need fast and effective techniques to maintain the quality. The present research aimed to identify, through image processing, damage caused to soybean seeds, namely the presence of greenish seeds and wrinkled seeds due to variations of humidity and temperature, where it was possible to identify greenish and wrinkled soybean seeds from images. Results obtained for greenish seeds indicated that the red color scale is the most suitable for selection due to its more significant variation compared to the other color scales. For the separation of wrinkled seeds, it can be stated that it is possible to find a selection parameter with 74.3% accuracy in removing seeds with medium to high degrees of wrinkle damage.
DOI: 10.6084/m9.figshare.14305328
title: Economic viability of a crop-livestock integration system

description: ABSTRACT: Crop monoculture and single conventional cattle raising were designed for a rapid increase in productivity and food supply. However, some of these production systems have shown signs of saturation and negative environmental impacts. Crop-livestock integration systems have been developed as an alternative that delivers increased productivity and greater environmental sustainability. This article presents an evaluation of the economic viability and the financial risk associated with an investment in crop-livestock integration with a focus on the production of beef cattle in São Carlos, SP, Brazil. Results showed that the investment is economically viable and has an Internal Rate of Return higher than that of a conventional system. Monte Carlo simulations showed that the integrated system presents lower market risks when compared to the conventional system.
DOI: 10.6084/m9.figshare.14305327
title: Producción de leche real vs la calculada a partir de la ENL estimada por el algoritmo de análisis de imágenes red-green-blue de gramíneas

description: ABSTRACT: Knowledge about the net lactation energy (NLE) contained in the dry matter of grasses is necessary to make decisions about forage and the balance of diets for grazing cattle. Its determination is made in laboratories using wet or dry chemistry methods, which are costly, delayed, and sometimes present sampling- or process-related reliability problems. An algorithm, which analyzes the red-green-blue (RGB) images of grasses taken by drone, has been developed as a technological alternative. This has allowed us estimating the NLE level, reducing costs, and changing the sampling system and analysis method. The objective of the present study was to compare the milk production, which was calculated from the NLE and estimated using the algorithm for analysis of RGB images of grasses (included in the TaurusWebs® software), vs the actual milk production. The study was conducted in 15 dairy farms belonging to the dairy control system of the Colácteos dairy cooperative, which are located in the upper tropical region (Department of Nariño, Colombia). The prairies evaluated were composed of mixtures of Kikuyo (Pennisetum clandestinum), Raigrás (Lolium spp), and False Poa (Holcus lanatus). The result was analyzed using a linear regression model (R²=0.86; R=0.93). In the Student´s t-test, the actual and estimated milk production averages were equal (P&gt;0.05). In conclusion, the NLE calculated using the algorithm satisfactorily explains the study livestock production, and the information generated by the algorithm can be used to calculate the NLE of grasses.
DOI: 10.6084/m9.figshare.14305325
title: Correlation of serum acute-phase proteins with bone repair in rabbits (Oryctolagus cuniculus) submitted to partial radio ostectomy

description: ABSTRACT: Bone trauma triggers an acute inflammatory response, which can alter the serum concentration of acute-phase proteins (PFA). We aimed to evaluate the effectiveness of PFA measurement as a laboratory biomarker for inflammation related to bone regeneration. A partial ostectomy of the right radius was performed on 22 young male rabbits. The complete blood count, albumin, ceruloplasmin, haptoglobin, C-reactive protein, and transferrin levels were recorded and correlated with the radiographic evolution of bone healing. Statistically the best correlations with the inflammatory process and radiological findings were reported with haptoglobin and transferrin compared to the other measured PFAs. Haptoglobin and transferrin showed a maximum peak 24 and 36 hours after surgery, respectively. Transferrin displayed signs of decrease in the first 6 hours after surgery, in advance of the leukocyte response. The measurement of acute-phase proteins proved to be viable, considering the conditions in which the present study was carried out. Among all variables studied, haptoglobin and transferrin showed best correlation with the inflammatory process of bone healing. Additional studies are needed to determine the sensitivity of acute-phase proteins as predictors of complications in the treatment of fractures.
DOI: 10.6084/m9.figshare.14305323
title: Management of white mold of soybean using winter cereal straw

description: ABSTRACT: The management of white mold (Sclerotinia sclerotiorum(Lib.) De Bary) has been one of the main production limitations faced by soybean (Glycine max L.) producers. Considering the complex management of this disease and resistance structure of the pathogen, the present study was conducted in the municipalities of Guarapuava and Palmas in Paraná with the objective of managing the white mold of soybean using straws of winter cereals, such as oat, rye, and triticale. Initially, the three winter cereals were cultivated simultaneously in both the study areas. Straw production, plant height, and shoot fresh and dry weight were evaluated. Subsequently, BMX Apollo soybean was cultivated on cereal straws, and the incidence and severity of white mold were evaluated. In the in vitro experiment, 20 sclerotia covered by a layer of cereal (oat, rye, and triticale) straws were added and carpogenic germination was observed only in the control treatment after 98 days. Regardless of the study site, rye presented greater height and fresh weight than the remaining two cereals. Soybean cultivation on winter cereal straw reduced the incidence and severity of white mold. Cultivation on rye straw reduced mold incidence by 77.7% and 76.6% in Palmas and Guarapuava, respectively.
DOI: 10.6084/m9.figshare.14305321
title: Cadmium, silicon and nutrient accumulation by maize plants grown on a contaminated soil amended with a diatomaceous Earth fertilizer

description: ABSTRACT: Given that cadmium (Cd) poses high persistence in the environment and toxicity to humans, strategies to either decrease or avoid Cd entry in the trophic chain are fundamental to secure food safety. Here we assessed the effects of applying rates of Si as a diatomaceous Earth-based fertilizer on the amelioration of Cd toxicity towards maize plants grown on soil with or without pH correction. Besides determining Si and Cd concentrations, we also evaluated plant accumulation of nutrients (N, P, K, Ca, Mg, Fe, Mn, Cu, and Zn) as a function of Si doses applied to the soil. Results showed that both the Si application and the liming had a positive effect on biomass and nutrient uptake, but the Si effect on plants’ performance took place irrespectively to the soil pH. Silicon ameliorated Cd phytotoxicity in both limed and unlimed soils by decreasing Cd concentration in shoots and improving biomass yield and plant nutrition. Silicon alleviation of Cd-inhibitory effects on plants was more effective in the unlimed soil owing to the higher Cd availability in acidic soils. Also, taking into account the reduced transfer of Cd to shoots driven by Si, lower Cd accumulation in maize grains is likely, with implications to food safety. So, further studies on field conditions are warranted.
DOI: 10.6084/m9.figshare.14305318
title: Zoonotic smooth and rough Brucella in dogs: seroprevalence and associated factors in an Atlantic Rainforest area of the state of Paraíba, Northeastern Brazil

description: ABSTRACT: Canine brucellosisis an infectious disease caused by bacteria of the genus Brucella, with world wide distribution and zoonotic impact, and in humans and animals is a neglected disease. In the present study, the sero prevalence of B. canis and B. abortus were determined in a probabilistic sample of housed dogs from the Atlantic Rainforest area of the state of Paraíba, Brazil, and the factors associated with sero positivity. A total of 386 dogs over three months of age were used. For the search for anti-B.canis antibodies the agar gel immune diffusion test (IDGA) was used as a screening and IDGA+2ME as confirmatory test, and to search for anti-B. abortus antibodies the Rose Bengal test (RBT) test was used. Apparent and real prevalences were calculated, and robust Poisson regression was used to identify factors associated with prevalence. The real prevalence fB. Canis was 12.6% and of B. abortus was 22.8%. The factors associated with sero positivity for B. canis were age greater than 10 years (prevalence ratio; PR = 6.38; P = 0.024) and dogs reared in they ard (PR = 5.20; P = 0.035) and for B. abortus was no treplacement of water of animals everyday (PR = 1.48; P = 0.033). It can be concluded that the prevalence of B. canis and B. Abortus in the region is high, which warns to the adopting of control and prevention measures, as well as greater care in the management of animals, especially for elderly dogs.
DOI: 10.6084/m9.figshare.14305316
title: Nutraceutical fruit characterization, nutritional aspects and sensory analysis of dovyalis jams

description: ABSTRACT: The objective of this study is to evaluate the composition, nutritional aspects and sensory analysis of the dovyalis jam. Dovyalis fruits were harvested from 4-year-old plants in the orchard of the Unioeste Experimental Farm. They were collected in the morning in polyethylene boxes, when they were ripe and with a purple coloration from the epicarp, being immediately transported to the Laboratory to prepare the jams. After fruits arrival, they were selected by size, color and weight and then sanitized in running water and the cups of the fruits were removed. The sensory evaluations of the jam samples were performed in a randomized fashion, with 30 volunteer tasters. The tasters were previously advised to evaluate the acceptance of the jams and their purchase intentions. Fruits in natura and dovyalis jam are sources of vitamin C. Dovyalis fruits can be used in the processing, due to its acidity. The jam prepared with dovyalis fruits without epicarp and endocarp, and less sugar, was more appreciated by the tasters.
DOI: 10.6084/m9.figshare.14305313
title: Nutrition and bioactive potential of the noni fruit cultivated from the Mato Grosso State

description: ABSTRACT: The objective was to evaluate the physical and chemical characteristics of noni (Morinda citrifolia) grown in the Mato Grosso State. Ripe fruits from a field located in Cuiabá-MT, had the peel, pulp and seeds separated and subjected to different evaluations. The fruit parts were characterized physically and chemically. Determinations of vitamin C, carotenoids, phenolics and the total antioxidant activity of the fruit mesocarp (pulp) were made. Noni cultivated in the Mato Grosso State presents high levels of potassium, calcium and sodium, with predominance of potassium. The protein content was higher in the seed, and the ash, in the peel, respectively. Noni pulp showed high levels of vitamin C, carotenoids and phenolics and can be considered a potential antioxidant.