cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/htfmc-crs57
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-25/3dimage_68623/</p>12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0008_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-25/3dimage_68623/12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0008.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-25/rawdata/12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0008_full.tiff,  file associated to 3D Image #68623, 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-25/file_15933/12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0008_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qrnh9-kkg45
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky. Used for CLEM
description: <strong>Tilt Series Date:</strong> 2011-12-15</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns_full.jpg: file associated to 3D Image #54420
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-2/rawdata/</p>11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns.st,  Tilt Series (Pixel Size 0.5 nm), 974.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-2/rawdata/11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 505.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-2/3dimage_54420/11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns_full.tiff,  file associated to 3D Image #54420, 2.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-15-2/file_13151/11dec15_CRYOSECS_pNLEGFP_grid2_0007_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qj4t4-9x813
title: HeLa CHMP4A+VPS4DN

description: HeLa cells transfected with CHMP4A+VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-07-23</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4DN obstruct provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-23-8/3dimage_68336/</p>11jul23_hela_4aDNlipo_0009_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 627.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-23-8/3dimage_68336/11jul23_hela_4aDNlipo_0009_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11jul23_hela_4aDNlipo_0009_ns.st,  Tilt Series (Pixel Size 0.5 nm), 768.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-23-8/rawdata/11jul23_hela_4aDNlipo_0009_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11jul23_hela_4aDNlipo_0009_ns_full.tiff,  file associated to 3D Image #68336, 727.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-07-23-8/file_15880/11jul23_hela_4aDNlipo_0009_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9sg20-06g72
title: AimB depletion control w/ xylose

description: Raw data files of AimB depletion control w/ xylose
description: <strong>Tilt Series Date:</strong> 2009-09-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> The AimB_depletion strain was constructed by John Werner, data collected by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity</p>
  zla2009-09-21-24_slicer10185.jpg: Caulobacter crescentus .  dividing
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-24/rawdata/</p>09sep21a_AimBxyl_042.mrc,  Tilt Series (Pixel Size 1.192 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-24/rawdata/09sep21a_AimBxyl_042.mrc"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_042_full.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-24/3dimage_10362/09sep21a_AimBxyl_042_full.rec"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_042.tif,  file associated to 3D Image #10362, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-24/file_2833/09sep21a_AimBxyl_042.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.3v67sd
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Zosterops natalis",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044038-250811113504898/datasets/export for details.
DOI: 10.22002/tez0p-0rp29
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch4_0031_full.jpg: file associated to 3D Image #54379
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-15/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch4_0031.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-15/rawdata/11dec12_cryosec_pnlegfp_grid3_Batch4_0031.st"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch4_0031_full.rec,  Reconstruction (Pixel Size 2.0 nm), 351.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-15/3dimage_54379/11dec12_cryosec_pnlegfp_grid3_Batch4_0031_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch4_0031_full.tiff,  file associated to 3D Image #54379, 1.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-15/file_13111/11dec12_cryosec_pnlegfp_grid3_Batch4_0031_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a4hj7-e9936
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-12-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-6/rawdata/</p>11dec22_C104_1_AT3BT1_0004_ns.st,  Tilt Series (Pixel Size 0.5 nm), 991.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-6/rawdata/11dec22_C104_1_AT3BT1_0004_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT3BT1_0004_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 456.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-6/3dimage_68111/11dec22_C104_1_AT3BT1_0004_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT3BT1_0004_ns_full.tiff,  file associated to 3D Image #68111, 712.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-6/file_15842/11dec22_C104_1_AT3BT1_0004_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t7d72-j0220
title: 1003_deletion

description: Raw data files of 1003_deletion
description: <strong>Tilt Series Date:</strong> 2009-10-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum</p>
<strong>Strain:</strong> AMB-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> Cells sent from Arash Komeili, data collected by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> (Could be) Stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1 or R2/2, 10nm gold, 100% humidity</p>
  zla2009-10-21-33_slicer7384.jpg: dividing cell? .  budding membrane
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-10-21-33/rawdata/</p>09oct21a_m1003_033.mrc,  Tilt Series (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-10-21-33/rawdata/09oct21a_m1003_033.mrc"
        > <i class="download icon"></i> Download </a></p>

        09oct21a_m1003_033_full.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-10-21-33/3dimage_9959/09oct21a_m1003_033_full.rec"
        > <i class="download icon"></i> Download </a></p>

        09oct21a_m1003_033.tif,  2.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-10-21-33/file_2799/09oct21a_m1003_033.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ra28z-5eq92
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-29/3dimage_69056/</p>12jan20_C110_3_pNL_INFalpha_AT1BT10003_full.rec,  Reconstruction (Pixel Size 2.0 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-29/3dimage_69056/12jan20_C110_3_pNL_INFalpha_AT1BT10003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_3_pNL_INFalpha_AT1BT10003.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-29/rawdata/12jan20_C110_3_pNL_INFalpha_AT1BT10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_3_pNL_INFalpha_AT1BT10003_full.tiff,  file associated to 3D Image #69056, 963.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-29/file_16105/12jan20_C110_3_pNL_INFalpha_AT1BT10003_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gwmfh-65v96
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-25/3dimage_68897/</p>12jan06_c105_1_4ADNCos7_AT3BT10008_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-25/3dimage_68897/12jan06_c105_1_4ADNCos7_AT3BT10008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3BT10008.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-25/rawdata/12jan06_c105_1_4ADNCos7_AT3BT10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3BT10008_full.tiff,  file associated to 3D Image #68897, 1.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-25/file_16034/12jan06_c105_1_4ADNCos7_AT3BT10008_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5rvk2-18v05
title: Chromogranin A-GFP

description: CLEM of chromogranin A-GFP expressing INS-1E cells
description: <strong>Tilt Series Date:</strong> 2014-08-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Chromogranin A GFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 68.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Friedberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both side pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads added to grids before freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-8/rawdata/</p>tomogram8.mrc,  Tilt Series (Pixel Size 0.96 nm), 861.6 MB  
        
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        tomogram8_full.rec,  Reconstruction (Pixel Size 3.947 nm), 223.7 MB  
        
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        tomogram8.rec,  Reconstruction (Pixel Size 3.84 nm), 110.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-8/3dimage_47850/tomogram8.rec"
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