DOI: 10.5061/dryad.xd2547dr6
title: Fast-spiking interneuron detonation drives high-fidelity inhibition in the olfactory bulb

description: Inhibitory circuits in the mammalian olfactory bulb (OB) dynamically
 reformat olfactory information as it propagates from peripheral receptors
 to the downstream cortex. To gain mechanistic insight into how specific OB
 interneuron types support this sensory processing, we examine unitary
 synaptic interactions between excitatory mitral and tufted cells (MTCs),
 the OB projection neurons and a conserved population of anaxonic external
 plexiform layer interneurons (EPL-INs) using pair and quartet whole-cell
 recordings in acute mouse brain slices. Physiological, morphological,
 neurochemical, and synaptic analyses divide EPL-INs into distinct subtypes
 and reveal that parvalbumin-expressing fast-spiking EPL-INs (FSIs)
 perisomatically innervate MTCs with release-competent dendrites and
 synaptically detonate to mediate fast, short-latency recurrent and lateral
 inhibition. Sparse MTC synchronization supralinearly increases this
 high-fidelity inhibition, while sensory afferent activation combined with
 single-cell silencing reveals that individual FSIs account for a
 substantial fraction of total network-driven MTC lateral inhibition. OB
 output is thus powerfully shaped by detonation-driven high-fidelity
 perisomatic inhibition.
description: # Fast-spiking interneuron detonation drives high-fidelity inhibition in
 the olfactory bulb
 [https://doi.org/10.5061/dryad.xd2547dr6](https://doi.org/10.5061/dryad.xd2547dr6) "NaN" = "Not a Number", used to indicate entries for which no data is available (e.g., if a neuron's physiological data was successfully recorded but its morphology was not recovered in the post hoc staining process, then morphological measurements for that neuron are listed as "NaN"). ### Fig 1 * Panels B-E * Fig1B.txt, Fig1C.txt, Fig1D.txt, Fig1E.txt * column 1: time (s): column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV) * Panel F * Fig1F.xls * Panels H inset, J inset * Fig1Hinset.txt, Fig1Jinset.txt * column 1: time (ms): column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV) * Panel K * Fig1K.xls * Panels L-O * Fig1Lleft.txt, Fig1Lright.txt, Fig1Mleft.txt, Fig1Mright.txt, Fig1Nleft.txt, Fig1Nright.txt, Fig1Oleft.txt, Fig1Oright.txt * column 1: time (ms); column 2: mean voltage (mV) or current (pA); columns 3-end: individual trials * Panels P-S * Fig1PQRS.xls ### Fig 2 * Panels C, D * Fig2CD.xls ### Fig 3 * Panel B * Fig3B.txt * column 1: time (s); column 2: FSI current (nA); column 3: MTC current (nA) * Panel C * Fig3C.txt * column 1: delta(t_spike) (ms); column 2: cross-correlation * Panel D * Fig3D.txt * column 1: time (ms); columns 2-17: EPSP voltage (mV); columns 18-31: detonation voltage (mV) * Panel E * Fig3E.txt * column 1: time (ms); column 2: delta(current) (pA) * Panel F * Fig3F.txt * column 1: time (ms); column 2: mean current (pA); columns 3-end: individual trials * Panels G, H * Fig3GH.xls ### Fig 4 * Panels B, D * Fig4Bleft.txt, Fig4Bright.txt, Fig4Dleft.txt, Fig4Dright.txt * column 1: time (ms); column 2: mean voltage (mV); columns 3-end: individual trials * Panels E, G, J * Fig4EGJ.xls * Panel H * Fig4H.txt * column 1: time (ms); column 2: EPSP trials, pre-PhTx-74, mean voltage (mV); columns 3-41: all trials, pre-PhTx-74; column 42: all trials, post-PhTx-74, mean voltage (mV); columns 43-81: all trials, post-PhTx-74 * Panel K * Fig4K.txt * column 1: time (ms); column 2: pre-PhTx-74, mean current (pA); columns 3-24: all trials, pre-PhTx-74; column 25: post-PhTx-74, mean current (pA); columns 26-47: all trials, post-PhTx-74 ### Fig 5 * Panels B, C, E, F * Fig5Bleft.txt, Fig5Bright.txt, Fig5Cleft.txt, Fig5Cright.txt, Fig5Eleft.txt, Fig5Eright.txt, Fig5Fleft.txt, Fig5Fright.txt * column 1: time (ms); column 2: mean current (pA); columns 3-end: individual trials * Panels G, H * Fig5GH.xls ### Fig 6 * Panels C, F, G * Fig6CFG.xls ### Fig 7 * Panel B * Fig7B.txt * column 1: time (ms); columns 2-9: voltage (mV), suprathreshold trials; columns 10-18: voltage (mV), subthreshold trials * Panel C * Fig7C.txt * column 1: time (ms); column 2: total lateral inhibitory current (pA); column 3: FSI-mediated lateral inhibitory current (pA) * Panel D * Fig7D_total.txt * column 1: time (ms); columns 2-end: total lateral inhibitory currents (pA) * Fig7D_FSI.txt * column 1: time (ms); columns 2-end: FSI-mediated lateral inhibitory currents (pA) * Fig7D.xls ### S1 Fig * Panels A, B * S1Fig.xls ### S2 Fig * Panels A, F, K, P * S2FigAFKP.txt * column 1: time (s); columns 2-5: voltage (mV) for panels A, F, K, P * S2FigAFKPinset.txt * column 1: time (s); columns 2-5: voltage (mV) for panels A, F, K, P * Panels B, G, L, Q * S2FigB.txt, S2FigG.txt, S2FigL.txt, S2FigQ.txt * column 1: time (ms); column 2: voltage (mV); column 3: current (pA) * S2FigBfit.txt, S2FigGfit.txt, S2FigLfit.txt, S2FigQfit.txt * column 1: time (ms); column 2: exponential fit * Panels C, H, M, R * S2FigCHMR.txt * column 1: time (ms); column 2: voltage (mV) for panels C, H, M, R * Panels D, E, I, J, N, O, S, T * S2FigDEIJNOST.xls ### S6 Fig * Panel A * S6FigA.txt * column 1: time (s): column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV) * Panel B * S6FigBleft.txt, S6FigBright.txt * column 1: time (ms); column 2: mean voltage (mV); columns 3-end: individual trials * Panel C * S6FigC.xls ### S7 Fig * Panels A-F * S7Fig.xls ### S8 Fig * Panels A, C, D, F * S8FigACDF.xls * Panel B * S8FigB_ctrl.txt, S8FigB_NA.txt, S8FigB_NAG.txt, S8FigB_wash.txt * column 1: time (ms); column 2: mean voltage (mV); columns 3-end: individual trials (mV) * Panel E * S8FigE_ctrl.txt, S8FigE_NA.txt, S8FigE_NAG.txt, S8FigE_wash.txt * column 1: time (ms); column 2: mean current (pA); columns 3-end: individual trials (pA) ### S9 Fig * Panels B, E * S9FigB.txt, S9FigE.txt * column 1: time (s); column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV) * Panels C, F * S9FigCleft.txt, S9FigCright.txt, S9FigFleft.txt, S9FigFright.txt * column 1: time (ms); column 2: mean voltage (mV) or current (pA); columns 3-end: individual trials ### S10 Fig * Panels A-G * S10Fig.xls ### S11 Fig * Panels A-D * S11Fig.xls ### S13 Fig * Panel B * S13FigB.txt * column 1: time (ms); columns 2-12: EPSP voltage (mV); columns 13-25: detonation voltage (mV) * Panel C * S13FigC.txt * column 1: time (ms); column 2: delta(current) (pA) * Panel E * S13FigE.txt * column 1: time (ms); columns 2-8: EPSP voltage (mV); columns 9-21: detonation voltage (mV) * Panel F * S13FigF.txt * column 1: time (ms); column 2: delta(voltage) (mV) ### S14 Fig * Panels A-F * S14Fig.xls ### S1 Table * S1Table.xls ### S2 Table * S2Table.xls