cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14288/1.0445209
title: Data from: A fish-focused menu: An interdisciplinary reconstruction of Ancestral Tsleil-Waututh diets

description: &lt;b&gt;Abstract&lt;/b&gt;&lt;br/&gt;&lt;p&gt;The study of past subsistence offers archaeologists a lens through which we can understand relationships between people and their homelands. Tsleil-Waututh Nation is a Coast Salish Nation whose traditional and unceded territory centres on Tsleil-Wat, Burrard Inlet, British Columbia, Canada. Tsleil-Waututh people are fish specialists whose traditional diet focuses primarily on marine and tidal protein sources. In this research we draw on the archaeological record, Tsleil-Waututh oral histories, community knowledge, fisheries ecology, and historical records to build an estimated pre-contact diet that ancestral Tsleil-Waututh people obtained from Tsleil-Wat. Based on prior archaeological research, we assume a high protein diet that is primarily (90-100%) from marine and intertidal sources. The four pillars of sTsleil-Waututh pre-contact diets (salmon, herring, clams, and waterfowl) offer anchor points that ensure the diet is realistic, evidence-based, and representative of community knowledge. We consider the caloric needs of adults, children, elders, and those who are pregnant or lactating. Finally, we consider the variation in the edible yield from different animal species and their relationships in the food web. Together, these data and anchor points build an estimated pre-contact diet averaged across seasons, ages, and biological sex from approximately 1000 CE up until early European contact in approximately 1792 CE. This work offers an empirically based reconstruction of Tsleil-Waututh lifeways and subsistence practices, which were based in a myriad of stewardship techniques, and that they were forced to halt due to colonial polices. This research offers a baseline that aids in understanding the pre-contact relationship between Tsleil-Waututh Nation and their territory.&lt;/p&gt;
DOI: 10.14288/1.0445249
title: Weak local adaptation to climate in seedlings of a deciduous conifer suggests limited benefits and risks of assisted gene flow

description: &lt;b&gt;Abstract&lt;/b&gt;&lt;br/&gt;&lt;p&gt;Assisted migration provides a potential solution to mitigate the increasing risks of forest maladaptation under climate change. Western larch (&lt;em&gt;Larix occidentalis Nutt&lt;/em&gt;.) is a deciduous conifer species undergoing assisted migration beyond its natural range in British Columbia into areas that have become suitable based on climatic niche modelling. We established a seedling common garden experiment in raised beds in a warm location outside the natural range for three growing seasons, with 52 natural populations from across the species range and 28 selectively-bred families from British Columbia. Intraspecific genetic variation in growth, phenology and cold hardiness was analyzed to test for signals of local adaptation and the effects of selective breeding to better understand the implications for assisted migration and breeding for future climates. We found weak differentiation among populations in all traits, with the proportion of additive genetic variance (QST) values ranging from 0.10 to 0.28. Cold hardiness had the weakest population differentiation and exhibited no clines with geographic or climatic variables. Selective breeding for faster growth has maintained genetic variation in bud flush phenology and cold hardiness despite delaying bud set. The weak signals of local adaptation we found in western larch seedlings highlights that assisted gene flow among populations is likely to have limited benefits and risks for mitigating maladaptation with climate change. Our findings suggest that assisted migration outside of the range and selective breeding may be important management strategies for western larch for future climates.&lt;/p&gt;
DOI: 10.14288/1.0445089
title: Improving Palliative Care Services in a BC First Nation

description: A BC First Nation has been able to come up with innovative solutions to address the increased healthcare demands such as improving high speed internet access to facilitate access to telehealth. However, there are still significant barriers that remain that make it difficult for the community to provide its surging palliative care population with optimal care. Indigenous patients already experience unique social, cultural and economic barriers to accessing quality healthcare, including palliative care. The modern day effects of colonialism, Indian hospitals, and residential schools are demonstrated through the health inequities present in Indigenous communities. Indigenous patients are more likely to have a chronic health condition such as diabetes, cancer, and have complications sooner that leads them to pursue palliative care. For many Indigenous communities, death is not just a physical process, but a social and spiritual event. As a result, significant improvements need to be made to change the course of declining palliative care services. According to our community partner, the deterioration in care is due to many factors such as communication breakdowns, lack of integration of Indigenous cultural practices and insufficient staffing support. As many of these deficiencies in care can be resolved through improved integration of the various palliative care services available within the community, our project aims to bridge the gap between the different healthcare facilities. These include the local health department, Vancouver Coastal Health (VCH) palliative care team, the pharmacy and local practitioners.
DOI: 10.14288/1.0445128
title: Data from: Robust phylogenomics settles controversies of classification and reveals evolution of male embolic complex of the Laufeia clade (Araneae, Salticidae, Euophryini)

description: &lt;b&gt;Abstract&lt;/b&gt;&lt;br/&gt;&lt;p&gt;The &lt;em&gt;Laufeia&lt;/em&gt; clade is a peculiar lineage of euophryine jumping spiders showing elevated divergence of male genitalic structures, especially the embolic complex that directly interacts with female genitalia during sperm transfer. With the rapid growth of species discovery in the &lt;em&gt;Laufeia&lt;/em&gt; clade, the controversy in its classification has become a crucial problem. In this study, we applied a phylogenomic approach using ultra-conserved element data to infer the phylogeny of the &lt;em&gt;Laufeia&lt;/em&gt; clade with extensive taxon sampling. A comparative morphological study was also performed to evaluate diagnostic characters and understand the evolution of male embolic complex within the &lt;em&gt;Laufeia &lt;/em&gt;clade. The results provide a strongly supported phylogenetic framework and updated generic concepts for the &lt;em&gt;Laufeia&lt;/em&gt; clade. The updated genera within the &lt;em&gt;Laufeia &lt;/em&gt;clade were all recovered as monophyletic with well-defined synapomorphies identified through character mapping on the phylogeny. Ancestral state reconstruction analyses revealed that the Type I embolic complex was ancestral and gradually evolved into the Type II and III embolic complex, and that some shapes of embolus often evolved multiple times independently in different lineages of the &lt;em&gt;Laufeia&lt;/em&gt; clade. The shift from foliage dwelling to tree trunk dwelling at the common ancestor of the &lt;em&gt;Laufeia &lt;/em&gt;clade may have facilitated the divergent evolution of male embolic complex in the &lt;em&gt;Laufeia &lt;/em&gt;clade. This study provides a solid foundation for future studies of systematics and evolution of this group.&lt;/p&gt;
DOI: 10.14288/1.0445211
title: Data from: Robust phylogenomics settles controversies of classification and reveals evolution of male embolic complex of the Laufeia clade (Araneae, Salticidae, Euophryini)

description: &lt;b&gt;Abstract&lt;/b&gt;&lt;br/&gt;&lt;p&gt;The &lt;em&gt;Laufeia&lt;/em&gt; clade is a peculiar lineage of euophryine jumping spiders showing elevated divergence of male genitalic structures, especially the embolic complex that directly interacts with female genitalia during sperm transfer. With the rapid growth of species discovery in the &lt;em&gt;Laufeia&lt;/em&gt; clade, the controversy in its classification has become a crucial problem. In this study, we applied a phylogenomic approach using ultra-conserved element data to infer the phylogeny of the &lt;em&gt;Laufeia&lt;/em&gt; clade with extensive taxon sampling. A comparative morphological study was also performed to evaluate diagnostic characters and understand the evolution of male embolic complex within the &lt;em&gt;Laufeia &lt;/em&gt;clade. The results provide a strongly supported phylogenetic framework and updated generic concepts for the &lt;em&gt;Laufeia&lt;/em&gt; clade. The updated genera within the &lt;em&gt;Laufeia &lt;/em&gt;clade were all recovered as monophyletic with well-defined synapomorphies identified through character mapping on the phylogeny. Ancestral state reconstruction analyses revealed that the Type I embolic complex was ancestral and gradually evolved into the Type II and III embolic complex, and that some shapes of embolus often evolved multiple times independently in different lineages of the &lt;em&gt;Laufeia&lt;/em&gt; clade. The shift from foliage dwelling to tree trunk dwelling at the common ancestor of the &lt;em&gt;Laufeia &lt;/em&gt;clade may have facilitated the divergent evolution of male embolic complex in the &lt;em&gt;Laufeia &lt;/em&gt;clade. This study provides a solid foundation for future studies of systematics and evolution of this group.&lt;/p&gt;
DOI: 10.14288/1.0445286
title: Dataset for: Optimizing Fish-Based Biomonitoring: Reliable Methods for Metal Concentration Analysis

description: This is the full data set associated with the following research paper: Optimizing Fish-Based Biomonitoring: Reliable Methods for Metal Concentration Analysis
DOI: 10.14288/1.0445357
title: iixsatimutilh - We Are Medicine For Eachother: A Traditional Medicines Program

description: Traditional medicines hold profound significance within numerous Indigenous communities, serving as integral components of cultural identity and holistic wellness practices. Traditional medicine assumes a central role in fostering the physician, mental, spiritual and emotional health and vitality of its members. An esteemed healer within the community, has devoted decades sharing her profound knowledge regarding the medicine that comes from the land, witnessing firsthand the transformative impact on individuals. She defines traditional medicine as a culmination of plants, healing, song, and dance. She emphasizes that traditional medicine and healing is about “the energy, the working and the sharing that all community members partake in to rid themselves of illness.” Much of this knowledge is passed down within the community by word-of-mouth, and thus a program is needed so that this wealth of knowledge can be taught and shared accurately and in a structured manner. She is dedicated to guiding learners on how to use traditional medicines and to understand when these medicines are the right option. Through her teachings, individuals are empowered to recognize the appropriateness of these remedies and engage in healing practices that resonate authentically with their cultural heritage and community traditions.
DOI: 10.14288/1.0447283
title: Lipopolysaccharide differentially alters systemic and brain glucocorticoid levels in neonatal and adult mice

description: We treated male and female C57BL/6J mice at postnatal day 5 (PND5) or PND90 with lipopolysaccharide (LPS; 50 µg/kg bw i.p.) or vehicle and collected blood and brain after 4 hr. We microdissected the prefrontal cortex, hippocampus, hypothalamus, and amygdala. We measured 7 steroids, including corticosterone, via liquid chromatography-tandem mass spectrometry and measured transcripts for key steroidogenic enzymes and hypothalamic-pituitary-adrenal axis components (Cyp11b1, Hsd11b1, Hsd11b2, Crh, Crhr1, Pomc) via qPCR.
DOI: 10.14288/1.0445287
title: Replication Data for: Expert assessments of maritime shipping decarbonization pathways by 2030 and 2050

description: International shipping conveys over 80% of global trade by volume and emits an estimated 3% of the world’s greenhouse gas (GHG) emissions. There are many potential pathways and barriers to decarbonizing the diverse and fragmented international shipping sector, with numerous uncertainties. Here, we employ expert elicitation, gathering perspectives from 149 world-leading experts in maritime shipping and decarbonization, to characterize uncertainties in shipping decarbonization pathways. These experts predict a 30-40% (25th – 75th percentile range) carbon intensity reduction by 2030 compared to 2008, meeting the International Maritime Organization’s (IMO) target. By 2050, they anticipate an approximate 40-75% cut in GHG emissions, falling short of the IMO’s 2050 net-zero GHG goal and thereby not aligning with a 1.5°C warming scenario. Responding experts see decarbonization occurring through three types of measures: operational, technological, and alternative energy sources. In the short-term, decarbonization is predicted to be dominated by operational measures, while in the long-term, it will be dominated by alternative energy, although there is no consensus on which fuels will dominate. Technological upgrades are expected to play crucial supporting roles. The experts believe that differences in business models and governance may lead to different decarbonization pathways by ship segment. The experts’ qualitative responses highlights: alternative energy systems, ship fleet turnover, spillover effects from land-based sectors, mitigation of industry pessimism, and the supply chain as critical leverage points that can propel shipping towards sustainable decarbonization pathways. Navigating this transition demands support from key levers identified in this study: politics and policy, maritime governance, and contractual architecture.
DOI: 10.14288/1.0445314
title: A wearable system for experimental knee pain during real-world locomotion: habituation and motor adaptation

description: These data are from a project exploring the pain habituation and motor adaptations that arise from an experimental knee pain model.
DOI: 10.14288/1.0445356
title: Cultural Harvest Program

description: The Cultural Harvest Program is a youth program aimed at enhancing the mental health and well-being of Indigenous youth in a rural first nations community. Rooted in traditional values and practices, the program aims to strengthen connections with culture, spirituality, and the land, ultimately reducing rates of youth incarceration and promoting healthy coping mechanisms. An environmental scan, which included both physical and digital resources, provided insights to inform program development. The program’s pilot phase will involve a small group of youth, with plans to expand to accommodate larger cohorts and a variety of land-based activities. By fostering cultural and social engagement, the Cultural Harvest Program seeks to empower the rural first nations youth to express their thoughts and emotions in a safe environment.
DOI: 10.14288/1.0445407
title: Hidden syndinian and perkinsid infections in dinoflagellate hosts

description: Associated paper abstract: Free-living core dinoflagellates are commonly infected by members of two parasitic clades that are themselves closely related to dinoflagellates, the “marine alveolates” (MALVs) and perkinsids. These parasites are abundant and ecologically important, but most species have been difficult to observe directly or cultivate, so our knowledge of them is usually restricted to environmental 18S rRNA gene sequences, and genome-scale molecular data are not available for most species. Here, we report numerous syndinian parasites and one parasite that is sister to all known perkinsids from isolated single cells of diverse, free-living dinoflagellates. Of the 14 infected host cells collected, only five were noticeably infected via light microscopy at the time of collection. Single-cell transcriptome sequencing yielded relatively high transcriptomic coverage for parasites as well as their hosts. Host and parasite homologs were distinguished phylogenetically allowing us to infer a robust phylogenomic tree based on 198 genes. The tree showed one parasite belongs to an undescribed lineage that is sister to perkinsids, whereas the remainder are members of the syndinian MALV II clade. Close relatives of all these parasites have been observed in 18S rRNA gene surveys, but until now none had been linked to a specific host. These findings illustrate the efficacy of single-cell isolation and transcriptome sequencing as a strategy for gaining deeper insights into the evolutionary history and host relationships of hidden single-celled parasites.
DOI: 10.14288/1.0445412
title: Admixture with cultivated sunflower likely facilitated establishment and spread of wild sunflower (Helianthus annuus) in Argentina

description: &lt;b&gt;Abstract&lt;/b&gt;&lt;br/&gt;&lt;p&gt;A better understanding of the genetic and ecological factors underlying successful invasions is critical to mitigate the negative impacts of invasive species. Here, we study the invasion history of &lt;em&gt;Helianthus annuus &lt;/em&gt;populations from Argentina, with particular emphasis on the role of post-introduction admixture with cultivated sunflower (also &lt;em&gt;H. annuus&lt;/em&gt;) and climate adaptation driven by large haploblocks. We conducted genotyping-by-sequencing of samples of wild populations as well as Argentinian cultivars and compared them with wild (including related annual Helianthus species) and cultivated samples from the native range. We also characterized samples for 11 known haploblocks associated with environmental variation in native populations to test whether haploblocks contributed to invasion success. Population genomics analyses supported two independent geographic sources for Argentinian populations, the central United States and Texas, but no significant contribution of related annual Helianthus species. We found pervasive admixture with cultivated sunflower, likely as result of post-introduction hybridization. Genomic scans between invasive populations and their native sources identified multiple genomic regions of divergence, possibly indicative of selection, in the invaded range. These regions significantly overlapped between the two native-invasive comparisons and showed disproportionally high crop ancestry, suggesting that crop alleles contributed to invasion success. We did not find evidence of climate adaptation mediated by haploblocks, yet outliers of genome scans were enriched in haploblock regions and, for at least two haploblocks, the cultivar haplotype was favoured in Argentina. Our results show that admixture with cultivated sunflower played a major role in the establishment and spread of &lt;em&gt;H. annuus&lt;/em&gt; populations in Argentina.&lt;/p&gt;
DOI: 10.14288/1.0445413
title: Data from: Does genome-wide variation and putatively adaptive variation identify the same set of distinct populations?

description: &lt;b&gt;Abstract&lt;/b&gt;&lt;br/&gt;&lt;p&gt;Identifying which populations within species to prioritize for conservation is a major challenge: one question is whether to prioritize populations based on adaptive variation versus considering genome-wide genetic variation. Many authors have advocated focusing solely on adaptive variation due to its direct connection to selection, function, and adaptive capacity. However, there are many limitations in identifying and using adaptive genetic variation for conservation. Patterns of genome-wide genetic variation may be congruent with patterns of adaptive genetic variation, and genome-wide variation is much easier to measure. However, evidence for congruence is mixed. We gather genome-wide and putatively adaptive SNP data across 34 species of plants and animals from published outlier and association studies to test congruence. We ask whether putatively adaptive subsets of genome-wide SNPs identify the same distinctive populations (measured using the Shapley Value of distinctiveness) as genome-wide SNPs. We find that genome-wide and putatively adaptive SNPs generally but variably agree on population prioritizations. As expected, the level of agreement is predicted by the proportion of putatively adaptive SNPs, and the agreement is lower when there is more overall population genetic structure. Interestingly, across our datasets, putatively adaptive SNPs do as well or better at predicting genome-wide population prioritization than sized-matched random subsets of SNPs. Taken together, using genome-wide genetic variation for population prioritization may be a generally sound and cost-effective strategy for prioritizing populations in order to safeguard species-level genetic variation.&lt;/p&gt;
DOI: 10.14288/1.0447735
title: Phylogenomics of coral-infecting corallicolids reveal multiple independent losses of chlorophyll biosynthesis in apicomplexan parasites

description: The transition from free-living to parasitic lifestyles induces major shifts in evolution, and nowhere is this more acute than in apicomplexans; obligate intracellular parasites of animals that evolved from photosynthetic algae. In other cases where photosynthesis has been lost, including most apicomplexans, chlorophyll is also absent, but in coral-infecting apicomplexans (corallicolids), chlorophyll biosynthesis genes are retained in the plastid genome despite their lack of photosystems. This suggests that the loss of photosynthesis and chlorophyll were decoupled in this lineage, but because these observations are only based on plastid genomes, two fundamental questions remain unclear. First, how this impacted apicomplexan evolution as a whole is unclear because there are conflicting phylogenetic positions for corallicolids: plastid gene phylogenies place them at the base of the apicomplexans whereas nuclear rRNA places them with late-branching coccidians. Second, it is unclear if chlorophyll or a metabolic intermediate is synthesized, as most chlorophyll biosynthesis enzymes are encoded in the nucleus. To address these questions we have sequenced transcriptomes from two corallicolids, infecting Parazoanthus swiftii and Madracis mirabilis. Phylogenomic data strongly support a late-branching relationship with coccidians, perhaps specifically to the protococcidians. We also find evidence for the expression of nucleus-encoded enzymes involved in chlorophyll biosynthesis in corallicolids and protococcidians. Overall, we conclude that photosynthesis was lost at the origin of apicomplexans, but chlorophyll synthesis was nonetheless retained through most of the early evolution of the group, and then lost approximately nine times independently, emphasizing the impact of parallel evolutionary changes in parasitic transitions.
DOI: 10.14288/1.0445425
title: Replication Data for: Satellite observations reveal stable forest limits and widespread shrub expansion across the Canadian forest-tundra ecotone

description: Sample datasets and R code supporting research titled "Satellite observations reveal stable forest limits and widespread shrub expansion across the Canadian forest-tundra ecotone"