cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j08051hz
title: CIL:36786, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Details of the origins of the cytopharyngeal ribbons and the quadrulus associated ribbons. At first view the cytopharyngeal ribbons look like they might arise next to basal bodies but it is clear that the cytopharyngeal ribbons arise only from the filamentous reticulum. The quadrulus associated ribbons appear to be analogous to the postciliary microtubules of the somatic ciliature that they curve posteriorly from the quadrulus. However quadrulus associated ribbons do pass very close or even touch the ends of the cytopharyngeal ribbons without becoming part of these latter ribbons. Discoidal vesicles bind to the anterior sides (observer‚Äôs left) of the cytopharyngeal ribbons and the smaller set of ribbons lies at its posterior side against the cytopharyngeal membrane. The tubular fingers of the alveoli pass between the cytopharyngeal ribbons next to the nodes of the filamentous reticulum from which the cytopharyngeal ribbons originate. Finally a row of parasomal sacs is seen along the dorsal edge of the quadrulus from which coated vesicles form. TEM taken on 5/23/73 by R. Wolf with Hitachi HU11A operating at 75kV. Neg. 16,250X. Bar = 0.5µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39176) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j00k27g7
title: CIL:36785, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39174) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Detail of the origin of the cytopharyngeal microtubules along the left side of the cytopharynx that arise within the filamentous reticulum in contact with its electron-opaque nodes. This system does not arise from the basal bodies of the quadrulus although microtubules do arise along the dorsal edge of the basal bodies of this membranelle and curve between the ends of the cytopharyngeal ribbons and the alveoli of the buccal cavity. The end of the alveoli marks the transition of the buccal cavity into the cytopharynx. As shown on the left of this figure the cytopharyngeal microtubules curve over the cytostomal cord. From here the ribbons fan out into the cytosol. They bind to discoidal vesicles, acidosomes and carrier vesicles and move these vesicles toward the cytopharynx. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 37,500X. Bar = 0.1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j07h1h7h
title: CIL:36780, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Sections show the origin of one cytopharyngeal ribbon of 12 microtubules from the nodes and show the tight curve the ribbon follows over the cytostomal cord. These ribbons move discoidal vesicles, acidosomes and carrier vesicles along one of their surfaces. Carrier vesicles are most abundant under the quadrulus where they arise from early endosomes and where the highest concentration of parasomal sacs and early endosomes occurs within the cell. These vesicles rapidly fuse with acidosomes. The acidosomes then dock at the membrane of the nascent phagosome. TEM taken on 4/10/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. Published in Poste and Nicolson (eds.) Membrane Fusion, Elsevier/North Holland Biomed. Press, p.735, 1978 and in J. Cell Biol. 1974. 63:904-22. Adapted with permission.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39164) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0pg1qmm
title: CIL:36778, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39162) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The three ciliary membranelles in the buccal cavity from which this class of ciliates gets its name, Oligohymenophorea, are each composed of four rows of basal body/cilium complexes. The quadrulus has four rows of cilia less tightly packed and interspersed with parasomal sacs. The dorsal and ventral peniculi are more tightly packed. In this micrograph only 6 or 7 rows of peniculi are present at this level since the rows of the peniculi do not go all the way to the posterior end but rows end in a staggered way somewhere along the length of the buccal cavity. The quadrulus borders the cytopharynx over its full length and goes to the posterior end of the buccal cavity. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,750X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0gx49h2
title: CIL:36776, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Posterior end of the buccal cavity with a nascent phagosome attached to the cytopharynx. The ribbed wall has ended and the cytopharynx is greatly reduced. The quadrulus goes to the end of the buccal cavity but the peniculi have ended, one or two rows of cilia at a time. The filamentous reticulum still covers the floor of the buccal cavity. Acidosomes are bound to the nascent phagosome and bundles of postoral microtubules lie close to the right edge of the developing phagosome. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 7,100X. Bar = 1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39161) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0833qzb
title: CIL:36774, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Cross section through the more anterior part of the oral region showing the four more loosely spaced rows of cilia making up the quadrulus and more compact cilia of the peniculi on the left hand wall of the buccal cavity. The indentation of the endoral membranelle lies to the right just above the buccal overture. The vestibulum bearing somatic ciliature connects the buccal cavity with the outside of the cell. The ribbed wall covers the upper right side of the buccal cavity. mac, macronucleus. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,850X. Bar = 1µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39155) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j01z4322
title: CIL:36724, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Monoclonal antibody, E30-2-1, was raised to antigen D6 that specifically labels acidosomes and DV-II. There was no label on the nascent vacuole membrane or ciliary membranes. This cell had been incubated in 1.1µm latex beads for 3 minutes and fixed without a chase. There is some gold over the cytosol but the nascent vacuole lumen is essentially free of label. TEM taken on 7/12/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm. Adapted with permission.
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density; intrinsic mass distribution
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40556) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0g15zsx
title: CIL:36722, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40555) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Immunogold label specific for Q2 antigen on discoidal vesicles. The same mAb used in CIL:36721 heavily labels discoidal vesicles and less heavily the cytopharyngeal membrane. A few gold particles are also found on the acidosomes. The distribution of this label confirms that the discoidal vesicles fuse with the cytopharynx to form the phagosome. TEM taken on 10/24/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibodies
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density; intrinsic mass distribution
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; gold; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0hd7tj0
title: CIL:36721, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A mAb, B25-1-3, was raised to antigen Q2 (arbitrary code) to the same set of membranes as mAb 132-F4-G6 labels. This mAb was more reactive on frozen sections. To tell the age of vacuoles, cells were exposed to latex beads for 2.5 minutes and then washed free of the beads (0.5 min) and then chased in bead-free buffer for a known time before fixing. In this image the labeled phagosome is a DV-I as it contained 1.1µm beads that were present in the medium for the 3 minutes before the cells were fixed. Some vesicles in the cytosol are also labeled but there is very little nonspecific label over the general cytosol or the contents of the vacuole. TEM taken 10/24/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibodies
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density; intrinsic mass distribution
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; gold; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0k0734d
title: CIL:36718, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Quick-freeze deep-etch images show a more three dimensional view of acidosomes surrounding a phagosome. Acidosomes are unique in that they contain indentations of their membrane. The lumen has a honeycombed appearance, also unique to these vesicles. Their E-face is almost free of IMPs while their P-face has many large particles which leave pits in the E-face. Acidosomes maintain a close association with the phagosome membrane. In this micrograph the difference between the lumens of pretrichocyst vesicles and acidosomes can be compared. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0j101tx
title: CIL:39181, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: The cytoproct is the cell‚Äôs defecation site where spent digestive vacuoles fuse with the plasma membrane. In this cell the cytoproct is closed and forms a ridge along the posterior suture on the ventral side of the cell. The ridge contains distinctive fibers not found in other parts of the cell. It is covered on its sides by alveoli but has a single membrane over the crest of the ridge. The somatic basal bodies on both sides of the ridge give rise to microtubular bundles that extend into the endoplasm where they lie against the surface of approaching spent vacuoles (DV-IV). The spent vacuole has tubular extensions protruding from its membrane. TEM taken on 4/13/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,250X. Adapted with permission.