DOI: 10.5061/dryad.msbcc2g1v
title: Data from: First evidence of WW superfemale for hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus)

description: Sex control is a promising approach for cost-effective aquaculture in
 certain fish species. Sturgeons possess ZZ/ZW genetic sex determination
 system and are popular aquaculture target for high-priced black caviar
 originated from female roe. Therefore, the production of all-female
 population is highly desired, but this has not yet been achieved to date.
 In this study, we identified a short nucleotide sequence specific to
 putative Z and W scaffolds of Acipenser ruthenus genome and developed the
 PCR to discriminate Z and W alleles. Using this PCR, A. ruthenus (N =
 1,280), A. baerii baerii (N = 66), and F2 of the bester hybrid (Huso huso
 × A. ruthenus: N = 31) were segregated into dimorphic pattern of a single
 Z amplicon and dual Z plus W amplicons, with approximately 1:1 ratio. Of
 those, the gonadal sexes were identified for 145 individuals, and all were
 concordant with their genetic sex. Additionally, we applied the
 Z/W-discriminative PCR for gynogenetic population of F3 bester and
 identified 25 out of 112 individuals (22.3%) showing only W amplicon,
 indicating that they are WW superfemales. These results will provide
 fundamental information to produce all-female sturgeons for aquaculture.
description: # Data from: First evidence of WW superfemale for hybrid sturgeon, the
 bester (Huso huso × Acipenser ruthenus)
 [https://doi.org/10.5061/dryad.msbcc2g1v](https://doi.org/10.5061/dryad.msbcc2g1v) In this study, we developed a PCR to discriminate Z/W sex chromosome for sturgeons. This dataset contains the individual results of genetic and gonadal sexing for three sturgeon spesies. ## Description of the data and file structure DATA & FILE OVERVIEW This dataset contains thee CSV files: genetic_and_gonadal_sexes_of_cultured_sturgeons.csv, individual data collected from the aquacultured sturgeons focusing on their genetic and gonadal sexes; gonadal_sexes_of_dead_Acipenser_ruthenus.csv, individual data collected from previously-genotyped Acipenser ruthenus and necropsied after death for gonadal sexing; genetic_sexes_of_chromosome_manipulated_bester.csv, individual data collected from chromosome-manipulated bester genotyped for comparison between experimental groups METHODOLOGICAL INFORMATION Description of methods used for collection/generation of data: genetic sex was determined by the presence/absence of Z/W amplicon from newly-developed Z/W-discrimitive PCR of sturgeons; gonadal sex was determined by a wet mount microscopy of dissected gonad (from Ar_AQ_0001 to Ar_AQ_1280, from Ar_DE_0001 to Ar_DE_0037), a gross observation of dissected gonad (from Ab_AQ_0001 to Ab_AQ_0066), or a gross observation of gonadal tissue sampled with a biopsy probe (from Be_AQ_0001 to Be_AQ_0031) Methods for processing the data: genotypes of the aquacultured sturgeons were inferred from the PCR results as follows, 'ZZ' for positive on Z amplicon and negative on W amplicon, 'ZW' for positive on Z amplicon and positive on W amplicon, 'WW' for negative on Z amplicon and positive on W amplicon (none for this study); genotypes of the chromosome-manipulated bester were categorized from the PCR results as follows, 'CatZ' for positive on Z amplicon and negative on W amplicon, 'CatZW' for positive on Z amplicon and positive on W amplicon, 'CatW' for negative on Z amplicon and positive on W amplicon DATA-SPECIFIC INFORMATION FOR: genetic_and_gonadal_sexes_of_cultured_sturgeons.csv Number of variables: 12 Number of cases/rows: 1,377 Variable List: 'individual_ID', identifier for individuals; 'species', scientific name of sturgeons ('bester_F2' means grandchilds of female Huso huso × male A. ruthenus); 'date_of_sampling', date of sampling for DNA source and gonadal sexing (shown in 'YYYY-MM-DD'); 'age', approximate age; 'folk_length_cm', folk length in centimeter; 'body_weight_g', body weight in gram; 'body_weight_kg', body weight in kilogram; 'date_of_PCR', date for conducting PCR (shown in 'YYYY-MM-DD'); 'PCR_Z_amplicon', 'positive' or 'negative' on the PCR amplicon detecting putative Z chromosome of sturgeons; 'PCR_W_amplicon', 'positive' or 'negative' on the PCR amplicon detecting putative W chromosome of sturgeons; 'inferred_genotype', 'ZZ' for positive on Z amplicon and negative on W amplicon, 'ZW' for positive on Z amplicon and positive on W amplicon; 'gonadal_sex', determined as 'ovary' or 'testis' or 'unidentified' Missing data codes: NA (data not collected) DATA-SPECIFIC INFORMATION FOR: gonadal_sexes_of_dead_Acipenser_ruthenus.csv Number of variables: 5 Number of cases/rows: 37 Variable List: 'individual_ID', identifier for individuals; 'species', scientific name of sturgeons; 'date_of_sampling', date of sampling for gonadal sexing (shown in 'YYYY-MM-DD'); 'inferred_genotype', 'ZZ' for positive on Z amplicon and negative on W amplicon, 'ZW' for positive on Z amplicon and positive on W amplicon, previously genotyped and divided into different aquaria; 'gonadal_sex', determined as 'ovary' or 'testis' (no 'unidentified' individuals) Missing data codes: (no missing data) DATA-SPECIFIC INFORMATION FOR: genetic_sexes_of_chromosome_manipulated_bester.csv Number of variables: 10 Number of cases/rows: 384 Variable List: 'individual_ID', identifier for individuals; 'species', scientific name of sturgeons ('bester_F3' means great-grandchilds of female Huso huso × male A. ruthenus); 'experimental_group', experimental groups as 'controls' or 'triploids' or 'haploids' or 'gynogens'; 'date_of_sampling', date of sampling for DNA source (shown in 'YYYY-MM-DD'); 'days_post_fertilization', days post fertilization at date of sampling ('3' and '5' for embryo and '20' for swimming-up fry); 'sire_ID', 'sire_1' or 'sire_2' for donor of semen (dam is common for all individuals); 'date_of_PCR', date for conducting PCR (shown in 'YYYY-MM-DD'); 'PCR_Z_amplicon', 'positive' or 'negative' on the PCR amplicon detecting putative Z chromosome of sturgeons; 'PCR_W_amplicon', 'positive' or 'negative' on the PCR amplicon detecting putative W chromosome of sturgeons; 'genotype_category', 'CatZ' for positive on Z amplicon and negative on W amplicon, 'CatZW' for positive on Z amplicon and positive on W amplicon, 'CatW' for negative on Z amplicon and positive on W amplicon Missing data codes: (no missing data)