DOI: 10.5061/dryad.66t1g1jxp
title: Drosophila suzukii wing spot size is robust to developmental temperature

description: Phenotypic plasticity is an important mechanism allowing adaptation to new
 environments and as such it has been suggested to facilitate biological
 invasions. Under this assumption, invasive populations are predicted to
 exhibit stronger plastic responses than native populations. Drosophila
 suzukii is an invasive species whose males harbor a spot on the wing tip.
 In this study, by manipulating developmental temperature, we compare the
 phenotypic plasticity of wing spot size of two invasive populations with
 that of a native population. We then compare the results with data
 obtained from wild-caught flies from different natural populations. While
 both wing size and spot size are plastic to temperature, no difference in
 plasticity was detected between native and invasive populations, rejecting
 the hypothesis of a special role of plasticity in the invasion success. In
 contrast we observed a remarkable stability in the spot-to-wing ratio
 across temperatures, as well as among geographic populations. This
 stability suggests either that the spot relative size is under stabilizing
 selection, or that its variation might be constrained by a tight
 developmental correlation between spot size and wing size. Our data show
 that this correlation was lost at high temperature, leading to an
 increased variation in the relative spot size, particularly marked in the
 two invasive populations. This suggests (i) that D. suzukii’s development
 is impaired by hot temperatures, in agreement with the cold-adapted status
 of this species; (ii) that the spot size can be decoupled from wing size,
 rejecting the hypothesis of an absolute constraint and suggesting that the
 wing color pattern might be under stabilizing (sexual) selection; (iii)
 that such sexual selection might be relaxed in the invasive populations.
 Finally, a subtle but consistent directional asymmetry in spot size was
 detected in favor of the right side in all populations and temperatures,
 possibly indicative of a lateralized sexual behavior.
description: Within this dataset you can find the analyses run in the
 paper. First, ./Sizes esimation shows the Python 3
 script to infer spot and wing sizes from the pictures
 Then, in ./R analyses you can find the R reports (with the
 analyses included) showing the particular<br> commands used for the
 analyses. Versions in .Rmd and .pdf are attached.
description: Here you can find data and scripts used in the article:
 Drosophila suzukii wing spot size is robust to developmental
 temperature <br> Before having a look at the
 files, please consider two things: 1) I've tried
 to upload the minimum amount of information neccesary to run all the
 analyses, in<br> order to make the replication as simple as possible
 and avoid possible sources of confusion. However,
 during this effort I might have left out something important unintendedly.
 If that's the case,<br> or if you struggle to understand
 something, please don't hesitate to contact me. 2)
 In the plasticity analyses you might find a population with a different
 name: Sokol. This is the name<br> the people collecting the samples
 gave to the population obtained in Dayton (Oregon). Because I learnt
 this<br> some time after I started the project I left the former
 name in these files, although I used to correct<br> place name in
 the manuscript. Sorry about that.