DOI: 10.35077/ace-dog
title: Baseline oxygen consumption decreases with cortical depth: Artery08_021722_Thy1_69

description: The cerebral cortex is organized in cortical layers that differ in their cellular density, composition, and wiring. Cortical laminar architecture is also readily revealed by staining for cytochrome oxidase – the last enzyme in the respiratory electron transport chain located in the inner mitochondrial membrane. It has been hypothesized that a high-density band of cytochrome oxidase in cortical layer IV reflects higher oxygen consumption under baseline (unstimulated) conditions. Here, we tested the above hypothesis using direct measurements of the partial pressure of O2 (pO2) in cortical tissue by means of 2-photon phosphorescence lifetime microscopy (2PLM). We revisited our previously developed method for extraction of the cerebral metabolic rate of O2 (CMRO2) based on 2-photon pO2 measurements around diving arterioles and applied this method to estimate baseline CMRO2 in awake mice across cortical layers. To our surprise, our results revealed a decrease in baseline CMRO2 from layer I to layer IV. This decrease of CMRO2 with cortical depth was paralleled by an increase in tissue oxygenation. Higher baseline oxygenation and cytochrome density in layer IV may serve as an O2 reserve during surges of neuronal activity or certain metabolically active brain states rather than baseline energy needs. Our study provides the first quantification of microscopically resolved CMRO2 across cortical layers as a step towards better understanding of the brain energy metabolism.
description: The MathWorks Matlab files (‘.mat’) are named after the cortical depth and contain Oxyphor2P decay data (‘Oxyphor2P.decay’), the derived pO2 values (‘Oxyphor2P.pO2Value’) and their coordinates (X: ‘Oxyphor2P.X_reference _coordinates’ and Y: ‘Oxyphor2P.Y_reference _coordinates’), which relate to the reference images with astrocyte-staining with SR101 (‘reference.SR101’), vasculature containing FITC (‘reference.FITC’) and the Oxyphor2P distribution (‘reference.Oxyphor2P’). DATASET METADATA: title:'Baseline oxygen consumption decreases with cortical depth: Artery08_021722_Thy1_69', Other:'Phosphorescence Lifetime Oxygen Imaging' SPECIMEN METADATA: LocalID:'Thy1_69', Species:'Mouse', NCBITaxonomy:'NCBI:txid10090', Age:'141.0', Ageunit:'Days', Sex:'Male', Genotype:'C57BL6', OrganName:'Brain', SampleLocalID:'Artery08_021722_Thy1_69', Locations:'somatosensory cortex' INSTRUMENT METADATA: MicroscopeType:'Two Photon Laser Scanning Microscopy', MicroscopeManufacturerAndModel:'Bruker Ultima', ObjectiveName:'Olympus UMPlanFL N, Olympus XLUMPlanFl', ObjectiveImmersion:'Water', ObjectiveNA:'0.5, 0.95', ObjectiveMagnification:'20x', DetectorType:'PMT', IlluminationTypes:'Chameleon Ultra femtosecond Ti:Sapphire laser (Coherent)' IMAGE METADATA: xAxis:'posterior-to-anterior', yAxis:'left-to-right', zAxis:'superior-to-inferior', Number:'1, 2, red, green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', Representation:'1: Oxyphor 2P; 2: FITC,', Flurophore:'Oxyphor2P, FITC-dextran, Alexa680-dextran', stepSizeX:'0.58 um, 1.16 um', stepSizeY:'0.58 um, 1.16 um', stepSizeZ:'3 um'