DOI: 10.5061/dryad.sn02v6xfp
title: Numeric data from: Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells

description: Stem and progenitor cell mitosis is essential for tissue development and
 homeostasis. How these cells ensure proper chromosome segregation, and
 thereby maintain mitotic fidelity, in the complex physiological
 environment of a living animal is poorly understood. Here we use in situ
 live-cell imaging of C. elegans germline stem and progenitor cells (GSPCs)
 to ask how the signaling environment influences stem and progenitor cell
 mitosis in vivo. Through a candidate screen we identify a new role for the
 insulin/IGF receptor (IGFR), daf-2, during GSPC mitosis. Mitosis is
 delayed in daf-2/IGFR mutants, and these delays require canonical,
 DAF-2/IGFR to DAF-16/FoxO insulin signaling, here acting cell
 non-autonomously from the soma. Interestingly, mitotic delays in
 daf-2/IGFR mutants depend on the spindle assembly checkpoint but are not
 accompanied by a loss of mitotic fidelity. Correspondingly, we show that
 caloric restriction, which delays GSPC mitosis and compromises mitotic
 fidelity, does not act via the canonical insulin signaling pathway, and
 instead requires AMP-activated kinase (AMPK). Together this work
 demonstrates that GSPC mitosis is influenced by at least two genetically
 separable signaling pathways and highlights the importance of signaling
 networks for proper stem and progenitor cell mitosis in vivo.
description: This dataset contains all numeric data reported in the paper
 "Non-autonomous insulin signaling delays mitotic progression in
 <em>C. elegans </em>germline stem and progenitor cells".
 Details on data collection and processing can be found in the methods
 section of our paper. Measurement data include the duration of mitosis in
 germline stem cells in a range of genetic backgrounds and/or experimental
 conditions, the number of nuclei and mitotic cells per germline
 proliferative zone and germ line and germline stem cell fluorescent
 intensity measurements.
description: # Numeric data for manuscript: Non-autonomous insulin signaling delays
 mitotic progression in C. elegans germline stem and progenitor cells
 [https://doi.org/10.5061/dryad.sn02v6xfp](https://doi.org/10.5061/dryad.sn02v6xfp) ## Description of the data and file structure These data were collected in support of the paper  "Non-autonomous insulin signaling delays mitotic progression in *C. elegans *germline stem and progenitor cells which is available on bioRxiv ([https://doi.org/10.1101/2024.06.28.601188](https://doi.org/10.1101/2024.06.28.601188)) and in press at PLoS Genetics. ### Files and variables #### File: Numeric\_Data\_for\_all\_figure\_panels.zip **Description:**  Data are organized by figure, with one folder per figure (e.g. Figure_1_RawData). Each folder contains one or more CSV files, with the file name indicating to which figure panel the numeric data belong. CSV files were generated by exporting data tables from Matlab. The first row gives the column name. Missing values are entered as NaN. Data are organized by Figure, with one folder per main/supplemental figure, except for Figure S2. Data for Figure S2 are published as a supplementary data table (S1 Data - Candidate screen mitotic features) with the paper above. There are a total of 10 folders. Each folder contains a variable number of CSV files. Each CSV file contains numeric data for one figure panel. If multiple measurements were plotted in one figure panel, there is one CSV file per measurement. In CSV files with a file name containing “ \_Animals”, data are the mean value for each animal. In CSV files with a file name containing “ \_Cells”, the measurements for each cell are listed. For example, Fig_1E_Animals_RawData.csv lists the mean duration of mitosis for each animal. Fig_1E_Cells_RawData.csv lists the duration of mitosis for all cells. In each CSV file, columns are labelled according to their contents. Some common column names include:  "strain" = the genetic strain for the associated measurement, following standard *C. elegans* nomenclature "gonad" or "germline" = the file name for the live-cell imaging recording for the associated measurement; one gonad was imaged per animal "cell" = the cell number within the associated gonad, for each measurement "DurCong" = the duration of mitosis per cell "mean_DurCong" = the mean duration of mitosis per animal "MI_pct" = the mitotic index as the percent of cells in mitosis per total number of nuclei in the proliferative/progenitor zone (PZ) of each animal "numMZnucFF" = the number of nuclei in the PZ of each animal "Micount" = the number of mitotic cells in the PZ of each animal ## Code/software No specific code is needed to view this data.