cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.1925027
title: Ouseley mission to Persia 1810-15 – Data on people mentioned and others of relevance

description: These files form part of an archive of research data relating to the diplomatic mission to Persia 1810-15, led by Sir Gore Ouseley as ambassador. The data have been compiled by independent researchers W H (Bill) Martin and Sandra Mason;  their contact details are in the README file..  The files comprise a number of searchable listings relating to the reports produced by members of the mission and other relevant data.  The data formed the basis for a published report on the Ouseley Mission referenced below, which is also part of the archive. 

This section of the archive contains a database, <em>ouspeople</em>, listing people mentioned in the reports on the Ouseley mission and others of relevance to the analysis.  The database file has been saved in two formats:  a Microsoft Access database (.accdb) and a comma-delimited text file (.txt).  The explanatory README text file contains a table showing the fields included in the database and giving definitions of them and of the codings used in different cases.
description: {"references": ["Martin W H & Mason S, The Ouseley brothers and their journey to Persia 1810-15:  Insights into the world of the traveller in the early nineteenth century  (Dry Drayton:  Leisure Consultants, 2018)."]}
DOI: 10.7910/dvn/28049/c38gsq
title: unhrc_1_20.csv
DOI: 10.7910/dvn/28275/3si5y3
title: MinSave_C83.bin.gz
DOI: 10.7910/dvn/28275/blhbk3
title: MinSave_C470.bin.gz
DOI: 10.7910/dvn/28275/ld8yld
title: MinSave_C84.bin.gz
DOI: 10.7910/dvn/28275/nwehue
title: MinSave_C657.bin.gz
DOI: 10.7910/dvn/28275/quk4ra
title: MinSave_C725.bin.gz
DOI: 10.7910/dvn/28275/tmvcav
title: MinSave_C645.bin.gz
DOI: 10.7910/dvn/28275/v28j0l
title: MinSave_C623.bin.gz
DOI: 10.7910/dvn/28275/wfqtba
title: MinSave_C427.bin.gz
DOI: 10.7910/dvn/28275/xbohbl
title: MinSave_C563.bin.gz
DOI: 10.7910/dvn/28275/yjpwjl
title: MinSave_C966.bin.gz
DOI: 10.7910/dvn/28275/5qfhxj
title: MinSave_C608.bin.gz
DOI: 10.7910/dvn/28275/7afzh9
title: MinSave_C716.bin.gz
DOI: 10.7910/dvn/28275/a9wz0z
title: MinSave_C860.bin.gz
DOI: 10.7910/dvn/28275/ggjahb
title: MinSave_C497.bin.gz
DOI: 10.7910/dvn/28275/j6hzc2
title: MinSave_C498.bin.gz
DOI: 10.7910/dvn/28275/sa1umi
title: MinSave_C788.bin.gz
DOI: 10.7910/dvn/28275/vsdtcr
title: MinSave_C610.bin.gz
DOI: 10.7910/dvn/28275/xofyyt
title: MinSave_C855.bin.gz
DOI: 10.7910/dvn/28275/1w7haa
title: MinSave_C641.bin.gz
DOI: 10.7910/dvn/28275/2i4vw4
title: MinSave_C517.bin.gz
DOI: 10.7910/dvn/28275/bcnflk
title: MinSave_C881.bin.gz
DOI: 10.7910/dvn/28275/ia09z4
title: MinSave_C780.bin.gz
DOI: 10.7910/dvn/28275/jvvid8
title: MinSave_C495.bin.gz
DOI: 10.7910/dvn/28275/qkbvxt
title: MinSave_C594.bin.gz
DOI: 10.7910/dvn/28275/qm1lne
title: MinSave_C477.bin.gz
DOI: 10.7910/dvn/28275/rzxbfm
title: MinSave_C453.bin.gz
DOI: 10.7910/dvn/28275/wnyll6
title: MinSave_C994.bin.gz
DOI: 10.7910/dvn/28275/y4v4xl
title: MinSave_C413.bin.gz
DOI: 10.17632/zkjf9w93rr.1
title: Data for: Why (field) experiments on unethical behavior are important: Comparing stated and revealed behavior

description: Data for: Why (field) experiments on unethical behavior are important: Comparing stated and revealed behavior
DOI: 10.5523/bris.2iopqddd82mvx2sa0i0lqx87jt
title: Alcohol pictorial health warning labels: the impact of self-affirmation and health warning severity

description: In this experimental human laboratory study, we examined whether enhancing self-affirmation among a population of drinkers reduces defensive reactions to health warnings and promote reactions related to behaviour change. We also examined how health warning severity influences these reactions. Participants (n=128) were randomised to a self-affirmation or control group. We tracked participants' eye movements and recorded self-reported responses to the health warnings (avoidance, reactance, effectiveness, susceptibility, and motivation to drink less), as well as self-efficacy to drink less. The study protocol was preregistered on the Open Science Framework (osf.io/x7vps/).
DOI: 10.7910/dvn/27638/j4zpl0
title: 3FFR.pdb
DOI: 10.7910/dvn/28036/povze8
title: replication.do
DOI: 10.7910/dvn/28275/1ikjta
title: MinSave_C810.bin.gz
DOI: 10.7910/dvn/28275/9jp0on
title: MinSave_C668.bin.gz
DOI: 10.7910/dvn/28275/kbvi3h
title: MinSave_C604.bin.gz
DOI: 10.7910/dvn/28275/khp06f
title: MinSave_C694.bin.gz
DOI: 10.7910/dvn/28275/n4cajz
title: MinSave_C398.bin.gz
DOI: 10.7910/dvn/28275/nloqdl
title: MinSave_C703.bin.gz
DOI: 10.7910/dvn/28275/qcnjtw
title: MinSave_C673.bin.gz
DOI: 10.7910/dvn/28275/wui5ui
title: MinSave_C60.bin.gz
DOI: 10.26186/5c0749572ead4
title: Earthquake sources of the Australian plate margin: data for the 2018 national tsunami and earthquake hazard assessments

description: This ecat record refers to the data described in ecat record 123048. The data, supplied in shapefile format, is an input to the 2018 National Seismic Hazard Assessment for Australia (NSHA18) product (ecat 123020) and the 2018 Probabilistic Tsunami Hazard Assessment for Australia (PTHA18) product (ecat 122789).
DOI: 10.5281/zenodo.1930381
title: Ouseley mission to Persia 1810-15 – Data on oriental manuscripts collected by the Ouseley brothers

description: These files form part of an archive of research data relating to the diplomatic mission to Persia 1810-15, led by Sir Gore Ouseley as ambassador. The data have been compiled by independent researchers W H (Bill) Martin and Sandra Mason;  their contact details are in the README file..  The files comprise a number of searchable listings relating to the reports produced by members of the mission and other relevant data.  The data formed the basis for a published report on the Ouseley Mission referenced below, which is also part of the archive. 

This section of the archive contains three databases, under the general heading of <em>ouscollect</em>, listing the main items in the Ouseley collections of oriental manuscripts now held at the Bodleian Library in Oxford.  Some of these items were undoubtedly acquired during the Ouseley mission to Persia 1810-15.  Other were obtained during the brothers earlier stays in India or through booksellers in London and elsewhere.  The database files have been saved in two formats:  a Microsoft Access database (<em>ouscollect.accdb</em>) covering all the Ouseley holdings, and three separated comma-delimited text files (<em>.txt</em>).  The explanatory README text file contains a table showing the fields included in the databases and giving definitions of them and of the codings used in different cases.
description: {"references": ["Martin W H & Mason S, The Ouseley brothers and their journey to Persia 1810-15:  Insights into the world of the traveller in the early nineteenth century  (Dry Drayton:  Leisure Consultants, 2018)."]}
DOI: 10.5281/zenodo.1930382
title: Ouseley mission to Persia 1810-15 – Data on oriental manuscripts collected by the Ouseley brothers

description: These files form part of an archive of research data relating to the diplomatic mission to Persia 1810-15, led by Sir Gore Ouseley as ambassador. The data have been compiled by independent researchers W H (Bill) Martin and Sandra Mason;  their contact details are in the README file..  The files comprise a number of searchable listings relating to the reports produced by members of the mission and other relevant data.  The data formed the basis for a published report on the Ouseley Mission referenced below, which is also part of the archive. 

This section of the archive contains three databases, under the general heading of <em>ouscollect</em>, listing the main items in the Ouseley collections of oriental manuscripts now held at the Bodleian Library in Oxford.  Some of these items were undoubtedly acquired during the Ouseley mission to Persia 1810-15.  Other were obtained during the brothers earlier stays in India or through booksellers in London and elsewhere.  The database files have been saved in two formats:  a Microsoft Access database (<em>ouscollect.accdb</em>) covering all the Ouseley holdings, and three separated comma-delimited text files (<em>.txt</em>).  The explanatory README text file contains a table showing the fields included in the databases and giving definitions of them and of the codings used in different cases.
description: {"references": ["Martin W H & Mason S, The Ouseley brothers and their journey to Persia 1810-15:  Insights into the world of the traveller in the early nineteenth century  (Dry Drayton:  Leisure Consultants, 2018)."]}
DOI: 10.5517/ccdc.csd.cc1k3d24
title: CCDC 1433225: Experimental Crystal Structure Determination

description: Related Article: M. Anjomshoa, M. Torkzadeh-Mahani, M. Sahihi, C. Rizzoli, M. Ansari, J. Janczak,  S. Sherafat Esfahani, F. Ataei, M. Dehkhodaei, B. Amirheidari|2018|CSD Communication|||
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.6084/m9.figshare.7109123
title: Interview - Christine Perakslis

description: This dataset 'Interview - Christine Perakslis’ is a component of a Doctor Of Philosophy conducted by candidate Alexander Hayes (3919493) titled 'The Socioethical Implications Of Body Worn Computers: An Ethnographic Study'. The full thesis can be accessed at https://ro.uow.edu.au/theses1/853<br>This research was conducted in the Faculty of Engineering and Information Systems, School of Computing and Information Technology (SCIT), University of Wollongong with research strength Innovation in Business & Social Research under Principal Supervisor Professor Katina Michael (University of Wollongong) and Associate Supervisor Dr Teemu Leinonen (Aalto University Finland). This thesis explores the socioethical implications of body worn computers (BWC) using an ethnographic approach. Furthermore, a subset, body worn cameras (BWCs), combines data with value added constancy through Location Based Services (LBS) over wireless network connections. The aim of this investigation was to engage global leaders from transdisciplinary stakeholder groups in semi-structured interviews, conversations and events, situating a review of the social impact and ethical implications of BWCs. A critical discourse analysis using a Foucauldian approach reveals power relations, which are then infused through narrative with unique intercultural perspectives, differentiating ‘location’ from ‘place’. The author of this study has subsequently identified through Grounded Theory that BWCs are causal agency for disconnect from proper culture which can be addressed through the application of Ngikalikarra, a unique framework for empathetic understanding of place and community engagement.
DOI: 10.7910/dvn/28275/3f14vu
title: MinSave_C459.bin.gz
DOI: 10.7910/dvn/28275/5auvyx
title: MinSave_C992.bin.gz
DOI: 10.7910/dvn/28275/i3qymp
title: MinSave_C708.bin.gz
DOI: 10.7910/dvn/28275/l60ejf
title: MinSave_C993.bin.gz
DOI: 10.7910/dvn/28275/p9xooc
title: MinSave_C965.bin.gz
DOI: 10.7910/dvn/28275/pygvws
title: MinSave_C697.bin.gz
DOI: 10.7910/dvn/28275/ufrth3
title: MinSave_C46.bin.gz
DOI: 10.7910/dvn/28275/uvyrom
title: MinSave_C648.bin.gz
DOI: 10.7910/dvn/28275/y6rb1o
title: MinSave_C7.bin.gz
DOI: 10.7910/dvn/28275/d9c9o3
title: MinSave_C589.bin.gz
DOI: 10.7910/dvn/28275/j7hwlv
title: MinSave_C504.bin.gz
DOI: 10.7910/dvn/28275/jkcifv
title: MinSave_C414.bin.gz
DOI: 10.7910/dvn/28275/lnfygo
title: MinSave_C92.bin.gz
DOI: 10.7910/dvn/28275/nlwmxk
title: MinSave_C556.bin.gz
DOI: 10.7910/dvn/28275/puhci3
title: MinSave_C63.bin.gz
DOI: 10.7910/dvn/28275/sqpzf2
title: MinSave_C583.bin.gz
DOI: 10.7910/dvn/28275/ssnrjg
title: MinSave_C848.bin.gz
DOI: 10.7910/dvn/28275/vvphy3
title: MinSave_C817.bin.gz
DOI: 10.7910/dvn/28275/yhdofp
title: MinSave_C545.bin.gz
DOI: 10.17632/z4mmp7sn3w.1
title: Data for: Impact of the Indian Summer Monsoon variability on the source area weathering in the Indo-Burman ranges during the last 21 ka– A sediment record from the Andaman Sea

description: Data from the Andaman Sea
DOI: 10.7910/dvn/28275/boyq6n
title: MinSave_C55.bin.gz
DOI: 10.7910/dvn/28275/h7ehto
title: MinSave_C571.bin.gz
DOI: 10.7910/dvn/28275/ixolrr
title: MinSave_C746.bin.gz
DOI: 10.7910/dvn/28275/lcbxrn
title: MinSave_C844.bin.gz
DOI: 10.7910/dvn/28275/mjnyhv
title: MinSave_C476.bin.gz
DOI: 10.7910/dvn/28275/mqrmde
title: MinSave_C911.bin.gz
DOI: 10.7910/dvn/28275/qccvd7
title: MinSave_C652.bin.gz
DOI: 10.7910/dvn/28275/yjacc1
title: MinSave_C620.bin.gz
DOI: 10.7910/dvn/28275/yopfo0
title: MinSave_C999.bin.gz
DOI: 10.5258/soton/d0729
title: Dataset: Modal Instabilities in High Power Fiber Laser Oscillators

description: Dataset supports
V. Scarnera et al "Modal Instabilities in High Power Fiber Laser Oscillators"
Optics Express 2018
DOI: 10.5525/gla.researchdata.686
title: Taenia multiceps coenurosis in Tanzania: a major and under-recognised livestock disease problem in pastoral communities
DOI: 10.7910/dvn/28275/gbgeko
title: MinSave_C783.bin.gz
DOI: 10.7910/dvn/28275/lsv6qy
title: MinSave_C720.bin.gz
DOI: 10.7910/dvn/28275/ng1aoj
title: MinSave_C568.bin.gz
DOI: 10.7910/dvn/28275/pf7rsp
title: MinSave_C885.bin.gz
DOI: 10.7910/dvn/28275/qph7qn
title: MinSave_C529.bin.gz
DOI: 10.7910/dvn/28275/u5nt5z
title: MinSave_C77.bin.gz
DOI: 10.7910/dvn/28275/utxtks
title: MinSave_C961.bin.gz
DOI: 10.7910/dvn/28275/zchhxy
title: MinSave_C505.bin.gz
DOI: 10.7910/dvn/28275/zla848
title: MinSave_C950.bin.gz
DOI: 10.7910/dvn/28275/7egc1x
title: MinSave_C820.bin.gz
DOI: 10.7910/dvn/28275/cl3abu
title: MinSave_C74.bin.gz
DOI: 10.7910/dvn/28275/ctyti7
title: MinSave_C565.bin.gz
DOI: 10.7910/dvn/28275/hcido9
title: MinSave_C727.bin.gz
DOI: 10.7910/dvn/28275/oitiru
title: MinSave_C435.bin.gz
DOI: 10.7910/dvn/28275/ttbggh
title: MinSave_C784.bin.gz
DOI: 10.7910/dvn/28275/uwtzce
title: MinSave_C971.bin.gz
DOI: 10.7910/dvn/28275/vulxro
title: MinSave_C588.bin.gz
DOI: 10.7910/dvn/28275/xeojp7
title: MinSave_C840.bin.gz
DOI: 10.18112/openneuro.ds001497.v1.0.1
title: Long-term Memory (LTM) for famous Faces, Places, and common Objects
DOI: 10.5281/zenodo.1957343
title: Fitacf data on 26 August 2018 and hdw file of Jiamusi HF data

description: The folder includes Fitacf data on 26 August 2018 and hdw file of Jiamusi HF radar.
DOI: 10.5281/zenodo.1957344
title: Fitacf data on 26 August 2018 and hdw file of Jiamusi HF data

description: The folder includes Fitacf data on 26 August 2018 and hdw file of Jiamusi HF radar.
DOI: 10.7910/dvn/28275/6jq7n0
title: MinSave_C808.bin.gz
DOI: 10.7910/dvn/28275/eexnak
title: MinSave_C636.bin.gz
DOI: 10.7910/dvn/28275/g5iqb7
title: MinSave_C907.bin.gz
DOI: 10.7910/dvn/28275/ly72bv
title: MinSave_C771.bin.gz
DOI: 10.7910/dvn/28275/mfxuwo
title: MinSave_C887.bin.gz
DOI: 10.7910/dvn/28275/ni1qpa
title: MinSave_C41.bin.gz
DOI: 10.7910/dvn/28275/pb7vzb
title: MinSave_C482.bin.gz
DOI: 10.7910/dvn/28275/skcnth
title: MinSave_C787.bin.gz
DOI: 10.7910/dvn/28275/smkgkf
title: MinSave_C656.bin.gz
DOI: 10.7910/dvn/28275/9jdcf2
title: MinSave_C56.bin.gz
DOI: 10.7910/dvn/28275/fpdark
title: MinSave_C811.bin.gz
DOI: 10.7910/dvn/28275/j8iwod
title: MinSave_C833.bin.gz
DOI: 10.7910/dvn/28275/jpwi1c
title: MinSave_C846.bin.gz
DOI: 10.7910/dvn/28275/ljgrqr
title: MinSave_C426.bin.gz
DOI: 10.7910/dvn/28275/pfgive
title: MinSave_C693.bin.gz
DOI: 10.7910/dvn/28275/tzewjx
title: MinSave_C630.bin.gz
DOI: 10.7910/dvn/28275/ul0yl6
title: MinSave_C794.bin.gz
DOI: 10.7910/dvn/28275/wz5ajm
title: MinSave_C736.bin.gz
DOI: 10.17632/bdrr5cwmsp.1
title: Data for: Thermal coefficients modification of high speed ball bearing by multi-object optimization method

description: Bearing cage and inner ring temperature at rotation speed 10000r/min
DOI: 10.7910/dvn/27601/saldmr
title: APEX_H2CO_merge_high_plait_all.fits
DOI: 10.7910/dvn/28275/4knzsy
title: MinSave_C670.bin.gz
DOI: 10.7910/dvn/28275/6loaie
title: MinSave_C700.bin.gz
DOI: 10.7910/dvn/28275/dxd4nm
title: MinSave_C509.bin.gz
DOI: 10.7910/dvn/28275/gll7zt
title: MinSave_C472.bin.gz
DOI: 10.7910/dvn/28275/hbtsmf
title: MinSave_C536.bin.gz
DOI: 10.7910/dvn/28275/hrhxhi
title: MinSave_C893.bin.gz
DOI: 10.7910/dvn/28275/nxwkov
title: MinSave_C484.bin.gz
DOI: 10.7910/dvn/28275/osgmue
title: MinSave_C821.bin.gz
DOI: 10.7910/dvn/28275/w4lr4z
title: MinSave_C501.bin.gz
DOI: 10.17632/my2xkg45f5.1
title: Data for: Statistical modeling of rates and trends in Holocene relative sea level

description: Holocene relative sea level proxy data from the east coast of the United States.
DOI: 10.7910/dvn/27595/axqjcn
title: legprof-components.v1.0.tab
DOI: 10.7910/dvn/28275/6wuotz
title: MinSave_C42.bin.gz
DOI: 10.7910/dvn/28275/brsxvl
title: MinSave_C557.bin.gz
DOI: 10.7910/dvn/28275/cmx1zm
title: MinSave_C523.bin.gz
DOI: 10.7910/dvn/28275/ht2cu3
title: MinSave_C600.bin.gz
DOI: 10.7910/dvn/28275/pm2o5m
title: MinSave_C97.bin.gz
DOI: 10.7910/dvn/28275/q7miij
title: MinSave_C947.bin.gz
DOI: 10.7910/dvn/28275/tpklqv
title: MinSave_C650.bin.gz
DOI: 10.7910/dvn/28275/tt5gwm
title: MinSave_C890.bin.gz
DOI: 10.7910/dvn/28275/xetsxa
title: MinSave_C531.bin.gz
DOI: 10.7910/dvn/28739/ykqtxq
title: i-data_confidential.zip
DOI: 10.5281/zenodo.1919006
title: Digital mapping of literature. Data, scripts and web

description: Data, scripts and web site of the project “Digital mapping of fictional places in Spanish Early Modern Byzantine novels”.  Visit the project web page at http://editio.github.io/mapping.literature
DOI: 10.5281/zenodo.1919007
title: Digital mapping of literature. Data, scripts and web

description: Data, scripts and web site of the project “Digital mapping of fictional places in Spanish Early Modern Byzantine novels”.  Visit the project web page at http://editio.github.io/mapping.literature
DOI: 10.7910/dvn/27595/gnaw5u
title: legprof-components.codebook.v1.0.pdf
DOI: 10.7910/dvn/28275/e4nuba
title: MinSave_C75.bin.gz
DOI: 10.7910/dvn/28275/f2ihk1
title: MinSave_C611.bin.gz
DOI: 10.7910/dvn/28275/fxn5yj
title: MinSave_C511.bin.gz
DOI: 10.7910/dvn/28275/i1cv1q
title: MinSave_C742.bin.gz
DOI: 10.7910/dvn/28275/j8ako2
title: MinSave_C68.bin.gz
DOI: 10.7910/dvn/28275/n9dnii
title: MinSave_C516.bin.gz
DOI: 10.7910/dvn/28275/oue79r
title: MinSave_C711.bin.gz
DOI: 10.7910/dvn/28275/vtu1vd
title: MinSave_C82.bin.gz
DOI: 10.7910/dvn/28275/wakl5k
title: MinSave_C831.bin.gz
DOI: 10.7910/dvn/28275/xyptjs
title: MinSave_C418.bin.gz
DOI: 10.7910/dvn/28739/nqtd0o
title: o-data_confidential.zip
DOI: 10.17632/7525wzpdnb.1
title: Data for: Numerical simulation of remote infrared imaging for marine exhaust system using wide-band k-distribution model combined with a new grouping method for spectral sub-intervals

description: Parameter databases of a model about remote infrared imaging calculation for combustion gases. The appended C source-code file is about how to use them to obtain model parameters under certain gas thermodynamic state.
DOI: 10.5281/zenodo.1494191
title: "Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion"

description: <strong>Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here, we show that HEV ORF3 protein forms membrane-associated oligomers. Immunoblot analyses of ORF3 protein expressed in cell-free <em>vs</em>. cellular systems suggested a posttranslational modification. Further analyses revealed that HEV ORF3 protein is palmitoylated at cysteine residues in its N-terminal region, as corroborated by <sup>3</sup>H-palmitate labeling, the investigation of cysteine-to-alanine substitution mutants and treatment with the palmitoylation inhibitor 2-bromopalmitate (2-BP). Abrogation of palmitoylation by site-directed mutagenesis or 2-BP treatment altered the subcellular localization of ORF3 protein, reduced the stability of the protein and strongly impaired the secretion of infectious particles. </strong><strong>Moreover, selective membrane permeabilization coupled with immunofluorescence microscopy revealed that HEV ORF3 protein is entirely exposed to the cytosolic side of the membrane, allowing to propose a model for its membrane topology and interactions required in the viral life cycle. </strong><strong>In conclusion, palmitoylation determines the subcellular localization, membrane topology and function of HEV ORF3 protein in the HEV life cycle. </strong>
description: This work was supported by the Swiss National Science Foundation (grants 31003A-156030 and 31003A-179424 to DM, as well as grants 156898 and 176393 to FGvdG), the Gilead Sciences International Research Scholars Program in Liver Disease (Award 2015 to JG), and benefited from funding from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement 340260 - PalmERa to FGvdG .
DOI: 10.7910/dvn/25543/tocqhf
title: Coppock Green Lab Field Replication Archive.zip
DOI: 10.7910/dvn/28275/05tlaf
title: MinSave_C897.bin.gz
DOI: 10.7910/dvn/28275/2ir4lx
title: MinSave_C585.bin.gz
DOI: 10.7910/dvn/28275/2uqh4u
title: MinSave_C701.bin.gz
DOI: 10.7910/dvn/28275/9ntrgt
title: MinSave_C997.bin.gz
DOI: 10.7910/dvn/28275/blssrl
title: MinSave_C863.bin.gz
DOI: 10.7910/dvn/28275/dq6jze
title: MinSave_C806.bin.gz
DOI: 10.7910/dvn/28275/lnqsgd
title: MinSave_C686.bin.gz
DOI: 10.7910/dvn/28275/rmonav
title: MinSave_C458.bin.gz
DOI: 10.5281/zenodo.1494192
title: "Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion"

description: <strong>Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here, we show that HEV ORF3 protein forms membrane-associated oligomers. Immunoblot analyses of ORF3 protein expressed in cell-free <em>vs</em>. cellular systems suggested a posttranslational modification. Further analyses revealed that HEV ORF3 protein is palmitoylated at cysteine residues in its N-terminal region, as corroborated by <sup>3</sup>H-palmitate labeling, the investigation of cysteine-to-alanine substitution mutants and treatment with the palmitoylation inhibitor 2-bromopalmitate (2-BP). Abrogation of palmitoylation by site-directed mutagenesis or 2-BP treatment altered the subcellular localization of ORF3 protein, reduced the stability of the protein and strongly impaired the secretion of infectious particles. </strong><strong>Moreover, selective membrane permeabilization coupled with immunofluorescence microscopy revealed that HEV ORF3 protein is entirely exposed to the cytosolic side of the membrane, allowing to propose a model for its membrane topology and interactions required in the viral life cycle. </strong><strong>In conclusion, palmitoylation determines the subcellular localization, membrane topology and function of HEV ORF3 protein in the HEV life cycle. </strong>
description: This work was supported by the Swiss National Science Foundation (grants 31003A-156030 and 31003A-179424 to DM, as well as grants 156898 and 176393 to FGvdG), the Gilead Sciences International Research Scholars Program in Liver Disease (Award 2015 to JG), and benefited from funding from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement 340260 - PalmERa to FGvdG .
DOI: 10.7910/dvn/28275/3mpekx
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