cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/5gfrz-xa755
title: Dividing Caulobacter

description: Dividing Caulobacter, low-quality tomograms
description: <strong>Tilt Series Date:</strong> 2015-03-10</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500.</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).  dosage: 180/A2. defocus: -10um. magnification: 27500.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-03-10-13</p>Qing15_10013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-03-10-13/rawdata/Qing15_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        Qing15_10013_part121_1.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-03-10-13/3dimage_53046/Qing15_10013_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25919/txgc-ad78
title: Parkes observations for project P737 semester 2023APRS_02

description: The aim of this proposal is to carry out tests of the new backend instruments which cannot be carried out during standard maintenance periods. The scheduled time is used to measure beam shapes, determine polarisation calibration solutions and commission the National Facility observing modes for the new wide-band receiver.
DOI: 10.22002/ymjmg-e9t02
title: Azospirillum Delta Operon 4

description: Raw data files of Azospirillum Delta Operon 4
description: <strong>Tilt Series Date:</strong> 2015-05-01</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Azospirillum brasilense</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Gladys Alexandre</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-01-13</p>DeltaOp4Day2May0008.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-01-13/rawdata/DeltaOp4Day2May0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        DeltaOp4Day2May0008_part121_10.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-05-01-13/3dimage_55572/DeltaOp4Day2May0008_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25919/k23f-e153
title: Atmospheric aerosol composition measurements from the RV Investigator voyage IN2021_V01 (CAPRIX)

description: This data collection contains aerosol measurements taken aboard the voyage IN2021_V01 as part of the Cloud Aerosol Precipitation Radiation Interactions eXperiment (CAPRIX, Australian Antarctic Division). This voyage, spanning January - March 2021, departed Hobart and travelled over the Southern Ocean, extending a latitude of 68.0025°S to 42.8864°S and longitude of 54.9051°E to 147.3803°E. Data includes offline filter analysis and online aerosol chemical composition.
DOI: 10.25919/wdfp-3n31
title: Parkes observations for project P1193 semester 2023APRS_02

description: In a search for steep spectrum sources in the combined data from the Rapid ASKAP Continuum Survey Low-band (RACS-low at 888 MHz) and high-band (RACS-high at 1667 MHz), we have found 14 compact objects with an ultra steep radio spectrum (\alpha &lt; -1.6) and matched with a Fermi unassociated source. Sources with ultra steep radio spectra and gamma-ray emission are likely to be previously unidentified pulsars. We propose to observe these targets with Murriyang (Parkes) UWL receiver. The deep targeted pulsar search will help us confirm new pulsars in extreme systems or environments, such as pulsars in compact binaries, and highly scattered pulsars.
DOI: 10.22002/dy65k-53f05
title: Oil well Actinobacterium

description: Grid box YWC208
description: <strong>Tilt Series Date:</strong> 2016-04-12</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Fixed cells received from Meng Wang/ Xiao-Lei Wu Lab at Peking University, China</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Firstly wash cells using PBS for 3 times, then add proper volum of 2.5% glutaraldehyde followed 1 hour room temp incubation (done in Peking), then send to Caltech for freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2016-04-12-3_slicer16841.jpg: conjugation?
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-12-3</p>20160410_YWC210_20004.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-12-3/rawdata/20160410_YWC210_20004.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160410_YWC210_20004_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-12-3/3dimage_74243/20160410_YWC210_20004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ee1wq-jvs58
title: Saccharomyces cerevisiae - 6210

description: Raw data files of Saccharomyces cerevisiae - 6210
description: <strong>Tilt Series Date:</strong> 2017-10-25</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain from Markus Babst @ University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to ~0.6 OD from overnight cultures in YPD.</p>
<strong>Sample Preparation:</strong> added ~3.5 µL of ~0.2 OD cells for each grid and blotted from behind for ~3 sec. FIB milled on Sep25_2017.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-9</p>TS8.mrc,  Tilt Series (Pixel Size 0.39 nm), 996.2 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-9/rawdata/TS8.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dqthx-zhf48
title: Vibrio cholerae C6706 ∆lacZ

description: Wild Type Vibrio
description: <strong>Tilt Series Date:</strong> 2015-06-02</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Simon Ringgaard</p>
  aba2015-06-02-20_slicer13878.jpg: pilus
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-06-02-20</p>VibrioLacZ0020.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-06-02-20/rawdata/VibrioLacZ0020.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioLacZ0020_part121_2.rec,  Reconstruction (Pixel Size 1.28 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-06-02-20/3dimage_57896/VibrioLacZ0020_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xfhf1-jkb18
title: Azospirillum delta Operon1 and 4

description: Raw data files of Azospirillum delta Operon1 and 4
description: <strong>Tilt Series Date:</strong> 2015-04-15</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Azospirillum brasilense</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Gladys Alexandre, University of Tennessee</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow overnight in 5 mL MMAB+N + 200 μg/mL Ampicillin at RT (on bench) without shaking- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N + 200 μg/mL Ampicillin- Leave at RT (on bench) without shaking overnight</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-14</p>AzoDEltoOp1Op4April0004.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-14/rawdata/AzoDEltoOp1Op4April0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        AzoDEltoOp1Op4April0004_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-14/3dimage_54090/AzoDEltoOp1Op4April0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.17632/4tpx96cbct.1
title: γ-PGA

description: near infrared spectroscopy of compound fertilizer
DOI: 10.22002/9vak3-v3m58
title: wt_serum_blotfrombehind

description: This is a sample of wt grown in serum bottle that made nanowires.
description: <strong>Tilt Series Date:</strong> 2015-07-26</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Sample Preparation:</strong> The sample was put on the C side (glow discharged side) on the left. The blotting was done from behind on the rt side. Vitrobot was used. All other vitrobot conditions are the same.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-25</p>10026.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-25/rawdata/10026.mrc"
        > <i class="download icon"></i> Download </a></p>

        10026_part61_4.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-25/3dimage_59602/10026_part61_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/aev4t-8zq75
title: INS-1E with Mito-DsRed

description: CLEM and ECT
description: <strong>Tilt Series Date:</strong> 2016-04-17</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 0.47368421 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cells frozen by Zach Freyberg at University of Pittsburgh.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-43</p>Mito-TS16.st,  Tilt Series (Pixel Size 0.6 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-43/rawdata/Mito-TS16.st"
        > <i class="download icon"></i> Download </a></p>

        Mito-TS16_part108_35.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-04-17-43/3dimage_74400/Mito-TS16_part108_35.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.17632/xcm34ztnzz.1
title: QCNN

description: Quaternion Convolutional Neural Network
DOI: 10.22002/mg4vp-5hh26
title: hns_overexpression_PS152

description: Andrew's DNA project
description: <strong>Tilt Series Date:</strong> 2016-08-24</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown for 27h from glycerol stock. At OD 0.51 induced with ~3mM IPTG and grown for 4h and froze as such, no spinning. OD was 0.52. Cell density and distribution looked good.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-48</p>hnsM9_PS152_atlas10006.mrc,  Tilt Series (Pixel Size 0.6 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-48/rawdata/hnsM9_PS152_atlas10006.mrc"
        > <i class="download icon"></i> Download </a></p>

        hnsM9_PS152_atlas10006_full.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-48/3dimage_78550/hnsM9_PS152_atlas10006_full.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2016-08-24-48_slicer17033.png,  thin flat pancake-shaped vesicles near the bottom surface of the cell, with stripes.  (There is a better picture of these at ps2016-08-24-42), 359.0 kB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-48_slicer17033.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5k71f-d4315
title: JV9114

description: JV9114: DotC-sfGFP fusion to the wild-type gene on the chromosome (thyA).
description: <strong>Tilt Series Date:</strong> 2017-07-08</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was 2.8</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9114 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. 3 grids with extra-thick carbon (JV9114T) and three finder grids (JV9114F). Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-08-71</p>area2-20170708-JV9114tomo0001p0007.mrc,  Tilt Series (Pixel Size 0.42 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-08-71/rawdata/area2-20170708-JV9114tomo0001p0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        area2-20170708-JV9114tomo0001p0007_part61_23.rec,  Reconstruction (Pixel Size 1.68 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-08-71/3dimage_86479/area2-20170708-JV9114tomo0001p0007_part61_23.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v2yd6-ytt29
title: Thermococcus koakarensis

description: Imaging Thermococcus koakarensis flagella
description: <strong>Tilt Series Date:</strong> 2016-09-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard Tk medium with starch and sulfur</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-19-11</p>atlas10012_part121_2.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-19-11/3dimage_79261/atlas10012_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas10012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-19-11/rawdata/atlas10012.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6220z-f9s64
title: A411V in vitro

description: A411V PEG in vitro sample7.5%PEG (in 1x P.P buffer) with 50µM CFA411V4Q, 12µM CheA, 20 µM CheW, 4% Trehalose [50µl total volume]
description: <strong>Tilt Series Date:</strong> 2015-07-09</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli in vitro</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Lynmarie Thompson</p>
description: Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-07-09-14</p>A411V_53mag0003.mrc,  Tilt Series (Pixel Size 0.206 nm), 1.1 GB  
        
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        A411V_53mag0003_full.rec,  Reconstruction (Pixel Size 0.824 nm), 688.9 MB  
        
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