cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0db820g
title: CIL:39964, Rattus norvegicus, astrocyte. In Cell Image Library

description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 4 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Alexa 488 (green) and Alexa 568 (red). Optical sections were generated with a single photon confocal microscope and compiled to produce this maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: Alexa Fluor 488; Alexa Fluor 568
Data qualification: Processed
description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 1 month old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Alexa 488 (green) and Alexa 568 (red). Optical sections were generated with a single photon confocal microscope and compiled to produce this maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j08k7979
title: CIL:39965, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Transgenic adult female mouse overexpressing alpha-synuclein [548 (f) transgenic from D-line] was anesthetized with nembutal, and perfused with 4% paraformaldehyde + 0.1% glutaraldehyde, followed by 1 hr. postfixation in 4% paraformaldehyde. Tissue was sectioned on a vibratome at a thickness of 80µm. Following rinses in phosphate buffer (PBS), sections were blocked (PBS with 3% NGS;1% fish gel, 0.1% Triton X-1000; 1% BSA) for 1 hour, followed by rinses in working buffer (1:10 blocking buffer: PBS). Primary antibody was diluted in working buffer (anti-alpha-SYN; Host = Rabbit used at 1:500), and tissue was covered with aluminum foil and incubated on a shaker in cold room overnight. Tissue was washed with working buffer and incubated in secondary antibody (goat anti-rabbit AlexaFluor 568, 1:50) covered with foil, on cold room shaker, 48 hrs. After rinses, 1:100 Hoescht 33342 was used to stain nuclei (30 min). After rinsing in PBS, sections were mounted on slides and coverslipped using Gelvatol. Sections were imaged using a BioRad RTS 2000MP Multiphoton, with a Nikon Plan Fluor 40X, NA 1.3 objective.
description: Image mosaic of a section through striatum showing immunofluorescence localization of alpha synuclein in a transgenic mouse overexpressing alpha synuclein. This mosaic image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database. For more information regarding this project, see: Price DL, Chow SK, MacLean NAB, Hakozaki H, Peltier S, Martone ME, Ellisman MH (2006) High-Resolution Large-Scale Mosaic Imaging using Multiphoton Microscopy to Characterize Transgenic Mouse Models of Human Neurological Disorders. Neuroinformatics. 2006;4(1):65-80.
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 568
Data qualification: Processed
DOI: 10.6075/j0x92bd6
title: CIL:39962, Rattus norvegicus, Purkinje cell, astrocyte, cerebellar granule cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: primary antibody plus labeled secondary antibody; Hoechst 33342
Data qualification: Processed;spatialmeasurements
description: 80µm thick sections were mounted in gelvitol using coverslips with a 1.0 µm thickness. Images were acquired with a BioRad RTS 2000MP Multiphoton microscope using a 40X 1.3NA oil immersion objective. This mosaic image was reconstructed with 10% overlap of individual images; image normalization was performed using ImageJ.
description: High resolution montage of a sagittal section through the cerebellar cortex of an adult male rat (80g), triple-labeled to reveal nuclei (Hoescht stain, blue), neurofilaments (particularly concentrated in axons, green) and astroglia (glial fibrillary acidic protein, GFAP, red). This image is a maximum projection of the Z-stack through the tissue, and has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j0222tw3
title: CIL:39960, Rattus norvegicus, Purkinje cell, astrocyte, cerebellar granule cell. In Cell Image Library

description: High resolution montage of a sagittal section through the cerebellar cortex of an adult male rat (80g), triple-labeled to reveal nuclei (Hoescht stain, blue), Purkinje neurons (IP3 receptor, green) and astroglia (glial fibrillary acidic protein, GFAP, red). This image is a maximum projection of the Z-stack through the tissue, and has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: primary antibody plus labeled secondary antibody; Hoechst 33342
Data qualification: Processed;spatialmeasurements
description: 80µm thick sections were mounted in gelvitol using coverslips with a 1.0 µm thickness. Images were acquired with a BioRad RTS 2000MP Multiphoton microscope using a 40X 1.3NA oil immersion objective. This mosaic image was reconstructed with 10% overlap of individual images; image normalization was performed using ImageJ.
DOI: 10.6075/j05t3kmh
title: CIL:39958, Rattus norvegicus, hippocampal neuron. In Cell Image Library

description: Postsynaptic densities (PSDs) in the CA1 region of the rat hippocampus, revealed by ethanolic phosphotungstic acid (EPTA) staining, were reconstructed from a maximum intensity projection of tomographic reconstruction (see, for example the blue arrow). Intensity was reversed so that PSDs appear bright against a dark background. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
 
 For more information see: Martone M. E.; Jones Y. Z.; Young S. J. Ellisman; M. H. Zivin; J. A. and Hu B. R. (1999) Modification of postsynaptic densities following transient cerebral ischemia:a quantitative and three dimensional ultrastructural study. J. Neurosci.19:1988-97.
description: Electron microscopic methods: Tissue sections from were stained either by 1% ethanolic phosphotungstic acid (E-PTA) (Bloom and Aghajanian, 1966, 1968) or by the conventional osmium-uranium-lead method. Briefly, coronal brain sections were cut at a thickness of 200 um with a Vibratome through the level of the dorsal hippocampus and post-fixed for 1 hr with 4% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4. For conventional osmium-uranium-lead staining, sections were post-fixed for 2 hr in 1% osmium tetroxide in 0.1 M cacodylate buffer, rinsed in distilled water, and stained with 1% aqueous uranyl acetate overnight. The tissue sections were then dehydrated in an ascending series of ethanol to 100%, followed by dry acetone, and embedded in Durupan ACM. Thin sections were counterstained with lead citrate before examination in the electron microscope. For E-PTA staining, sections were dehydrated in an ascending series of ethanol to 100% and stained for 1 hr with 1% PTA prepared by dissolving 0.1 gm of PTA in 10 ml of 100% ethanol and adding four drops of 95% ethanol. Sections were then embedded in Durcupan ACM. The tomogram was generated from single tilt images that spanned -60 to 60° in 2° increments, acquired with a using an JEOL4000 IVEM. Magnification, 20000.0 X; accelerating voltage, 400 KeV.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: illumination by electrons
Visualization methods: tungsten compound
DOI: 10.6075/j0ns0v12
title: CIL:39963, Rattus norvegicus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Tomographic reconstruction of a portion of medium spiny neuron from the neostriatum of a Sprague Dawley adult rat, in a 4 um thick section. This data set is poorly aligned due to a lack of adequate fiducial markers. This volume is an example of a poor reconstruction. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
description: The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -66 to 70° in 2° increments. Magnification, 10000X; accelerating voltage, 3.0 MeV.
description: Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Data qualification: Processed
DOI: 10.6075/j07d2v70
title: CIL:39952, Mus musculus, Purkinje cell. In Cell Image Library

description: Tissue was perfused with paraformaldehyde, sectioned (100 µm) on a vibratome and mounted in gelvatol, and imaged on a Biorad Radiance 2000 Confocal using a 60x 1.4NA objective.
description: Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed
description: A Purkinje neuron from the cerebellum of a 2 month old male C57BL/6 mouse, injected with Lucifer Yellow. The elaborate, highly branched dendritic arbor is revealed in this maximum projection image which was generated by compiling a z-series of optical sections. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j0gt5n9v
title: CIL:39951, Rattus norvegicus, CNS neuron (sensu Vertebrata), Purkinje cell. In Cell Image Library

description: Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Data qualification: Processed
description: Specimen was imaged using a BioRad MRC 1024 Confocal, using a 63X NA 1.4 objective and 488 laser line.
description: A portion of dendrite and associated spines from a Purkinje neuron of the rat cerebellum. This image is a maximum intensity projection through a computationally deblurred transmitted light series of optical sections. The through focus series was taken from a 2 um section prepared for electron microscopy.
DOI: 10.6075/j0mk6d04
title: CIL:39950, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

description: Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Data qualification: Processed
description: Tomographic reconstruction of a dendrite and associated dendritic spines from a medium spiny neuron from the mouse nucleus accumens. This image is a maximum intensity projection of a segment of dendrite in a 2 um section. The cell was filled by intracellular injection of Lucifer Yellow dye and photooxidized. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
description: The tomogram was generated using an JEOL4000 IVEM. Single tilt images spanned -60 to 60° in 2° increments. Magnification, 3000X; accelerating voltage, 400KeV.
DOI: 10.6075/j04m94ng
title: CIL:39944, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library

description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7–11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0vd6zjc
title: CIL:39943, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library

description: Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7–11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j03n23hn
title: CIL:39953, Mus musculus, Purkinje cell. In Cell Image Library

description: Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed
description: Tissue was perfused with paraformaldehyde, sectioned (100 µm) on a vibratome and mounted in gelvatol, and imaged on a Biorad Radiance 2000 Confocal using a 60x 1.4NA objective.
description: A Purkinje neuron from the cerebellum of a 2 month old male C57BL/6 mouse, injected with Lucifer Yellow. The elaborate, highly branched dendritic arbor is revealed in this maximum projection image which was generated by compiling a z-series of optical sections. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j03x86r2
title: CIL:39941, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements
description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7–11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
DOI: 10.6075/j0h41rj4
title: CIL:39939, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library

description: Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
description: Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7–11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements