DOI: 10.5061/dryad.zcrjdfn8s
title: Data for: Parallel processing of quickly and slowly mobilized reserve vesicles in hippocampal synapses

description: Vesicles within presynaptic terminals are thought to be segregated into a
 variety of readily releasable and reserve pools. The nature of the pools
 and trafficking between them is not well understood, but pools that are
 slow to mobilize when synapses are active are often assumed to feed pools
 that are mobilized more quickly, in a series. However,
 electrophysiological studies of synaptic transmission have suggested
 instead a parallel organization where vesicles within slowly and quickly
 mobilized reserve pools would separately feed independent reluctant- and
 fast-releasing subdivisions of the readily releasable pool. Here we use
 FM-dyes to confirm the existence of multiple reserve pools at hippocampal
 synapses and a parallel organization that prevents intermixing between the
 pools, even when stimulation is intense enough to drive exocytosis at the
 maximum rate. The experiments additionally demonstrate extensive
 heterogeneity among synapses in the relative sizes of the slowly and
 quickly mobilized reserve pools, which suggests equivalent heterogeneity
 in the numbers of reluctant and fast-releasing readily releasable vesicles
 that may be relevant for understanding information processing and storage.
description: # Data for: Parallel processing of quickly and slowly mobilized reserve
 vesicles in hippocampal synapses Data from individual preparations are
 contained in separate directories, each with a unique identifier; there
 are 191 in total. Each contains either two or three files (.tif, .sts, and
 usually .vls). The .tif file is a stack of aligned images. The .sts file
 contains two columns. The numbers in the first column are the median
 values of the mean value of several ROIs for each image. The second column
 is the mean value of an area designated as background. The .vls file
 contains 1 column for each ROI and the numbers are the mean values. The
 preparations used for generating each figure are listed below. Please
 don't hesitate to contact me at
 [john.wesseling@csic.es](mailto:john.wesseling@csic.es) for help. ##
 Figure 2 ### Figure2A (Example) E3_09092021 ### Figure2B-E E2_08092021
 E2_09092021 E3_09092021 E4_09092021 E5_09092021 E3_10092021 E4_10092021
 ### Figure 2–Figure Supplement 2 ### Three20HzTrains E7_25032022
 E1_25032022 E5_25032022 E1_24032022 E206042022 E6_06042022 ###
 Two20HzTrains E4_25032022 E2_25032022 E8_25032022 E6_25032022 E2_24032022
 E5_24032022 E4_24032022 E4_06042022 E5_06042022 E2_07042022 ## Figure 3
 ### A, Example E5_13102023 ### B-D n = 8 preparations E3_18092023
 E1_19092023 E4_20092023 E1_21092023 E2_10102023 E3_10102023 E5_10102023
 E5_13102023 ## Figure 4 ### Figure 4a (1 min) D250816 D26082016 A29082016
 D29082016 ### Figure 4b (3 min) E24082016 C2_250816 C26082016 F26082016
 ### Figure 4c (8 min) E25082016 B260816 B29082016 E29082016 ## Figure 5
 E1_08022022 E3_08022022 E4_08022022 E3_09022022 E5_09022022 E6_09022022 ##
 Figure 6 E3_29072022 E6_29072022 E7_29072022 E4_29072022 E8_1_29072022
 E5_29072022 ## Figure 7 E2_08112021 E2_09112021 E3_09112021 E2_10112021
 E3_10112021 E4_10112021 E5_10112021 E3_11112021 E4_11112021 E6_11112021
 E1_12112021 E2_12112021 E3_12112021 ## Figure 8 ### 12sAT20Hz (a)
 E1_11012022 E4_11022022 E4_15022022 C03022015 A04022015 B04022015
 C04022015 ### 60sAt20Hz (b) E2_11022022 E1_14022022 E7_14022022
 E1_15022022 D21012015 ### 60sat1Hz (c) E2_10022022 E5_14022022 E2_15022022
 E5_15022022 E2_14022022 A02022015 B03022015 ### Figure 8–Figure Supplement
 2 ### Load1Hz E1_14122020 E3_16122020 E1_18122020 E2_18122020 ### Load20Hz
 E3_14122020 E1_17122020 E2_17122020 E5_17122020 ## Figure 9 ### Load
 FM4-64 at 20Hz then 1-43 at 1Hz, Measure FM1-43 (n=8) E2_09122020
 E3_10122020 E5_10122020 E1_11122020 E2_12122020 E4_12122020 E2_13122020
 E4_13122020 ### Load FM4-64 at 20Hz then 1-43 at 1Hz, Measure FM4-64 (n=6)
 E1_26012021 E1_27012021 E3_27012021 E5_28012021 E1_29012021 E3_29012021
 ### Load FM1-43 at 20Hz then 4-64 at 1Hz, Measure FM1-43 (n=7) E2_10122020
 E2_11122020 E1_12122020 E3_12122020 E5_12122020 E1_13122020 E3_13122020
 ### Load FM1-43 at 20Hz then 4-64 at 1Hz, Measure FM4-64 (n=6) E2_26012021
 E2_27012021 E4_27012021 E4_28012021 E2_29012021 E4_29012021 ## Figure 10
 E1_08092021 E6_09092021 E5_10092021 ### Figure 10–Figure Supplement 1 ###
 A. 1Hz for 10 min then 20 Hz for 12 s n = 4 E2_12072021 E5_12072021
 E3_15072021 E4_15072021 ### B. 20Hz for 20s, then 13 min then 20Hz for 12s
 n = 3 E4_12072021 E2_15072021 E6_15072021 ### 10 min delay then 20Hz for
 20s, then 3 min then 20Hz for 12s n = 3 E3_12072021 E6_12072021
 E5_15072021 ## Figure 11 ### Panel A E5_11062021 E1_07072021 E2_07072021
 E5_07072021 E2_08072021 E1_23072021 E2_23072021 E6_23072021 E1_29072021
 E2_29072021 E4_16082021 E3_17082021 ### Panel B E4_11062021 E4_08072021
 E4_23072021 E4_29072021 E3_16082021 E2_17082021 E5_17082021 E8_17082021
 ### Panel D E4_02082021 E5_02082021 E2_03082021 E2_01102021 E1_13102021
 E3_19102021 E4_19102021 E1_20102021 E2_20102021 E3_20102021 ### Figure
 11–Figure supplement 1 E6_11062021 E6_07072021 E3_08072021 E3_23072021
 E2_16082021 E4_17082021 ### Figure 11–Figure Supplement 2 E5_07042022
 E6_07042022 E1_12042022 E4_12042022 E3_12042022 E5_12042022 E7_12042022 ##
 Figure 12 E3_01032021 E5_02032021 E1_03032021 E3_03032021 E4_04032021 ###
 Figure 12–Figure Supplement 1 ### Measure FM464 E1_11032021 E1_12032021
 E2_15032021 E6_15032021 ### Measure FM143 E3_12032021 E5_12032021
 E1_15032021 E3_15032021