cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6968168
title: Mass fingerprinting and electrophysiological analysis of the venom from the scorpion Centruroides hirsutipalpus (Scorpiones: Buthidae)

description: Abstract Background Centruroides hirsutipalpus, of the family Buthidae, is a scorpion endemic to the Western Pacific region of Mexico. Although medically important, its venom has not yet been studied. Therefore, this communication aims to identify their venom components and possible functions. Methods Fingerprinting mass analysis of the soluble venom from this scorpion was achieved by high-performance liquid chromatography and electrospray mass spectrometry. Furthermore, the soluble venom and its toxic effects were evaluated extensively via electrophysiological assays in HEK cells expressing human voltage-gated Na+ channels (hNav 1.1 to Nav1.6), CHO cells expressing hNav 1.7, potassium channel hERG 1 (Ether-à-go-go-related-gene) and the human K+-channel hKv1.1. Results The separation of soluble venom produced 60 fractions from which 83 distinct components were identified. The molecular mass distribution of these components varies from 340 to 21,120 Da. Most of the peptides have a molecular weight between 7001 and 8000 Da (46% components), a range that usually corresponds to peptides known to affect Na+ channels. Peptides with molecular masses from 3000 to 5000 Da (28% of the components) were identified within the range corresponding to K+-channel blocking toxins. Two peptides were obtained in pure format and completely sequenced: one with 29 amino acids, showing sequence similarity to an "orphan peptide" of C. limpidus, and the other with 65 amino acid residues shown to be an arthropod toxin (lethal to crustaceans and toxic to crickets). The electrophysiological results of the whole soluble venom show a beta type modification of the currents of channels Nav1.1, Nav1.2 and Nav1.6. The main effect observed in channels hERG and hKv 1.1 was a reduction of the currents. Conclusion The venom contains more than 83 distinct components, among which are peptides that affect the function of human Na+-channels and K+-channels. Two new complete amino acid sequences were determined: one an arthropod toxin, the other a peptide of unknown function.
DOI: 10.6084/m9.figshare.6968171
title: Acute kidney injury following radical cystectomy and urinary diversion: predictors and associated morbidity

description: ABSTRACT Introduction: Acute kidney injury (AKI) after major surgeries is associated with significant morbidity and mortality. We aim to report incidence, predictors and associated comorbidities of AKI after radical cystectomy in a large cohort of patients. Materials and Methods: We conducted a retrospective analysis of 1000 patients who underwent open radical cystectomy in a tertiary referral center. Perioperative serum creatinine measurements were used to define AKI according to the RIFLE criteria (as Risk, Injury and Failure). The predictors of AKI after surgery were determined using univariate and multivariate analyses. Results: Out of 988 evaluable patients, AKI developed in 46 (4.7%). According to RIFLE criteria; AKI-Risk, AKI-Injury and AKI-Failure occurred in 26 (2.6%), 9 (0.9%) and 11 (1.1%) patients, respectively. Multivariate analysis showed that performing nephroureterectomy with cystectomy (Odds ratio [OR]: 4.3; 95% Confidence interval [CI]: 1.3-13.6; p=0.01) and the development of high grade complications (OR: 3.8; 95% CI 1.9-7.2; p&lt;0.0001) were independently associated with AKI. Conclusions: AKI is a significant morbidity after radical cystectomy and the term should be included during routine cystectomy morbidity assessment.
DOI: 10.6084/m9.figshare.6968168.v1
title: Mass fingerprinting and electrophysiological analysis of the venom from the scorpion Centruroides hirsutipalpus (Scorpiones: Buthidae)

description: Abstract Background Centruroides hirsutipalpus, of the family Buthidae, is a scorpion endemic to the Western Pacific region of Mexico. Although medically important, its venom has not yet been studied. Therefore, this communication aims to identify their venom components and possible functions. Methods Fingerprinting mass analysis of the soluble venom from this scorpion was achieved by high-performance liquid chromatography and electrospray mass spectrometry. Furthermore, the soluble venom and its toxic effects were evaluated extensively via electrophysiological assays in HEK cells expressing human voltage-gated Na+ channels (hNav 1.1 to Nav1.6), CHO cells expressing hNav 1.7, potassium channel hERG 1 (Ether-à-go-go-related-gene) and the human K+-channel hKv1.1. Results The separation of soluble venom produced 60 fractions from which 83 distinct components were identified. The molecular mass distribution of these components varies from 340 to 21,120 Da. Most of the peptides have a molecular weight between 7001 and 8000 Da (46% components), a range that usually corresponds to peptides known to affect Na+ channels. Peptides with molecular masses from 3000 to 5000 Da (28% of the components) were identified within the range corresponding to K+-channel blocking toxins. Two peptides were obtained in pure format and completely sequenced: one with 29 amino acids, showing sequence similarity to an "orphan peptide" of C. limpidus, and the other with 65 amino acid residues shown to be an arthropod toxin (lethal to crustaceans and toxic to crickets). The electrophysiological results of the whole soluble venom show a beta type modification of the currents of channels Nav1.1, Nav1.2 and Nav1.6. The main effect observed in channels hERG and hKv 1.1 was a reduction of the currents. Conclusion The venom contains more than 83 distinct components, among which are peptides that affect the function of human Na+-channels and K+-channels. Two new complete amino acid sequences were determined: one an arthropod toxin, the other a peptide of unknown function.
DOI: 10.6084/m9.figshare.6968171.v1
title: Acute kidney injury following radical cystectomy and urinary diversion: predictors and associated morbidity

description: ABSTRACT Introduction: Acute kidney injury (AKI) after major surgeries is associated with significant morbidity and mortality. We aim to report incidence, predictors and associated comorbidities of AKI after radical cystectomy in a large cohort of patients. Materials and Methods: We conducted a retrospective analysis of 1000 patients who underwent open radical cystectomy in a tertiary referral center. Perioperative serum creatinine measurements were used to define AKI according to the RIFLE criteria (as Risk, Injury and Failure). The predictors of AKI after surgery were determined using univariate and multivariate analyses. Results: Out of 988 evaluable patients, AKI developed in 46 (4.7%). According to RIFLE criteria; AKI-Risk, AKI-Injury and AKI-Failure occurred in 26 (2.6%), 9 (0.9%) and 11 (1.1%) patients, respectively. Multivariate analysis showed that performing nephroureterectomy with cystectomy (Odds ratio [OR]: 4.3; 95% Confidence interval [CI]: 1.3-13.6; p=0.01) and the development of high grade complications (OR: 3.8; 95% CI 1.9-7.2; p&lt;0.0001) were independently associated with AKI. Conclusions: AKI is a significant morbidity after radical cystectomy and the term should be included during routine cystectomy morbidity assessment.
DOI: 10.6084/m9.figshare.6968162
title: Prevalence of visual disorders in school children

description: ABSTRACT Objective: To evaluate the epidemiological profile of visual acuity (VA) dysfunction, the possible ocular disorders and the frequency of ophthalmologic care of school children from the city of Itaúna, Minas Gerais, Brazil. Methods: A cross-sectional study with a population of 432 students in the public schools. The individuals were evaluated by the Snellen method and the sample age ranged from 4 to 17 years. Data were collected and systematized. Those who had VA ≤ 0.7 in at least one eye were referred to the ophthalmology service. Results: Of the 432 students evaluated in this study, 14.5% presented low VA, when evaluated by the Snellen test. Of these, 61.9% were female. The age group with the highest prevalence of low vision were schoolchildren aged 15 to 17 years. The majority presented alteration in both eyes and 60% of the evaluated students stated that they had never had an ophthalmological consultation. Of the students who attended the consultations, most were diagnosed with refractive errors and needed optical correction. Conclusion: The results showed that a significant portion of the individuals evaluated had low vision and required ophthalmologic referral. In addition, there was no previous ophthalmological consultation in most of the schoolchildren. This fact reinforces to public health agencies, the need to implement visual screening systems in schools and offer assistance to these students, with the aim of improvements in their learning and quality of life
DOI: 10.6084/m9.figshare.6968162.v1
title: Prevalence of visual disorders in school children

description: ABSTRACT Objective: To evaluate the epidemiological profile of visual acuity (VA) dysfunction, the possible ocular disorders and the frequency of ophthalmologic care of school children from the city of Itaúna, Minas Gerais, Brazil. Methods: A cross-sectional study with a population of 432 students in the public schools. The individuals were evaluated by the Snellen method and the sample age ranged from 4 to 17 years. Data were collected and systematized. Those who had VA ≤ 0.7 in at least one eye were referred to the ophthalmology service. Results: Of the 432 students evaluated in this study, 14.5% presented low VA, when evaluated by the Snellen test. Of these, 61.9% were female. The age group with the highest prevalence of low vision were schoolchildren aged 15 to 17 years. The majority presented alteration in both eyes and 60% of the evaluated students stated that they had never had an ophthalmological consultation. Of the students who attended the consultations, most were diagnosed with refractive errors and needed optical correction. Conclusion: The results showed that a significant portion of the individuals evaluated had low vision and required ophthalmologic referral. In addition, there was no previous ophthalmological consultation in most of the schoolchildren. This fact reinforces to public health agencies, the need to implement visual screening systems in schools and offer assistance to these students, with the aim of improvements in their learning and quality of life
DOI: 10.6084/m9.figshare.6968156
title: CHARACTERISTICS FOR THE SELECTION OF PARENTS OF Corymbia citriodora AIMING TO THE PRODUCTION OF WOOD AND ESSENTIAL OIL

description: ABSTRACT This study aimed to identify the most important characteristics for the selection of parent trees of Corymbia citriodora by estimating genetic dissimilarity and linear correlation degree for wood and essential oil production. This study was conducted in experimental areas of the company JAMP Florestal -located in Dueré-TO -and at Universidade Federal do Tocantins (Federal University of Tocantins), campus Gurupi, from January to April 2014, in 19 parent trees based on 19 characteristics. With the data, the following steps were performed: clustering through Tocher’s optimization method; calculation of genetic dissimilarity between parents; quantification of relative contribution; and verification of correlation degree existing among the characteristics evaluated through Pearson correlation using the program GENES. The characteristics essential oil yield and heartwood diameter presented the major contributions for genetic dissimilarity. Thus, it must be prioritized for wood production: trees with lower values in crown height and sapwood diameter; and trees with higher values in total height, commercial height, diameter at breast height and stump diameter. For essential oil production, it must be prioritized trees with lower values in leaf width, bark thickness and sapwood diameter; and trees with higher values in leaf nitrogen content, leaf potassium content, total green biomass and total dry biomass, essential oil yield, essential oil content and crown volume.
DOI: 10.6084/m9.figshare.6968153
title: MORPHOLOGICAL, PHYSICAL AND THERMAL CHARACTERIZATION OF MICROFIBRILLATED CELLULOSE

description: ABSTRACT The aim of this study was to characterize microfibrillated cellulose (MFC) produced with a Masuko Sangyo Super Masscolloider using bleached and unbleached pulp of Eucalyptus spp. The MFC was characterized regarding morphology (TEM), crystallinity, viscosity, zeta potential and thermal properties (TGA). Regardless of the fiber type, the processing dramatically reduced the dimensions of the material, so as to obtain structures with nanometric dimensions. MFC produced with unbleached pulp preserved the original brown color of the pulp, which may be advantageous for some applications in the packaging sector, while films produced with bleached pulp were more translucent. The MFC showed lower values of viscosity and crystallinity index in relation to cellulosic pulp. The zeta potential was influenced by the type of fiber used. The main thermal transitions in MFC occurred after 200 ºC, demonstrating the potential of this material forhigh-temperature applications.
DOI: 10.6084/m9.figshare.6968156.v1
title: CHARACTERISTICS FOR THE SELECTION OF PARENTS OF Corymbia citriodora AIMING TO THE PRODUCTION OF WOOD AND ESSENTIAL OIL

description: ABSTRACT This study aimed to identify the most important characteristics for the selection of parent trees of Corymbia citriodora by estimating genetic dissimilarity and linear correlation degree for wood and essential oil production. This study was conducted in experimental areas of the company JAMP Florestal -located in Dueré-TO -and at Universidade Federal do Tocantins (Federal University of Tocantins), campus Gurupi, from January to April 2014, in 19 parent trees based on 19 characteristics. With the data, the following steps were performed: clustering through Tocher’s optimization method; calculation of genetic dissimilarity between parents; quantification of relative contribution; and verification of correlation degree existing among the characteristics evaluated through Pearson correlation using the program GENES. The characteristics essential oil yield and heartwood diameter presented the major contributions for genetic dissimilarity. Thus, it must be prioritized for wood production: trees with lower values in crown height and sapwood diameter; and trees with higher values in total height, commercial height, diameter at breast height and stump diameter. For essential oil production, it must be prioritized trees with lower values in leaf width, bark thickness and sapwood diameter; and trees with higher values in leaf nitrogen content, leaf potassium content, total green biomass and total dry biomass, essential oil yield, essential oil content and crown volume.
DOI: 10.6084/m9.figshare.6968150
title: STUDY OF MACHINING OF THERMAL RECTIFICATED Corymbia citriodora AND Pinus elliottii

description: ABSTRACT This study evaluated the influence of thermal rectification in machining Corymbia citriodora and Pinus elliottii. The heat treatment was carried out for 6 hours at temperatures of 100°C, 140°C, 160°C, 180°C and 200°C and evaluated the performance of machining in three different material cutting tools, polished and hard metal carbide with TiB2 coating. The cutting power and roughness Ra and Rt of the machined surfaces with three different tools were evaluated. The power has proven lower for Pinus elliottii species. The roughness was lower for Corymbia citriodora wood. The roughness Ra and Rt were lower for heat treatment temperature of 180°C.
DOI: 10.6084/m9.figshare.6968153.v1
title: MORPHOLOGICAL, PHYSICAL AND THERMAL CHARACTERIZATION OF MICROFIBRILLATED CELLULOSE

description: ABSTRACT The aim of this study was to characterize microfibrillated cellulose (MFC) produced with a Masuko Sangyo Super Masscolloider using bleached and unbleached pulp of Eucalyptus spp. The MFC was characterized regarding morphology (TEM), crystallinity, viscosity, zeta potential and thermal properties (TGA). Regardless of the fiber type, the processing dramatically reduced the dimensions of the material, so as to obtain structures with nanometric dimensions. MFC produced with unbleached pulp preserved the original brown color of the pulp, which may be advantageous for some applications in the packaging sector, while films produced with bleached pulp were more translucent. The MFC showed lower values of viscosity and crystallinity index in relation to cellulosic pulp. The zeta potential was influenced by the type of fiber used. The main thermal transitions in MFC occurred after 200 ºC, demonstrating the potential of this material forhigh-temperature applications.
DOI: 10.6084/m9.figshare.6968147
title: GENETIC DIVERGENCE IN POPULATIONS OF Caryocar brasiliense Camb. FROM THE PHYSICAL CHARACTERISTICS OF THE FRUITS

description: ABSTRACT The study of the genetic and phenotypic variability of the populations of Caryocar brasiliense Camb. assists in strategies for conservation and breeding of the specie. The objective of this work was to characterize the genetic diversity through the physical characteristics of pequi fruits of two populations through uni and multivariate analysis, as well as the implications in their domestication and breeding. Fruits were harvested from 20 mother trees (ten fruits per mother tree) from two populations in the state of Minas Gerais (Curvelo and São Gonçalo do Rio Preto Municipalities) during the years 2010, 2011 and 2012. The data were submitted to analysis of variance using a hierarchical classification model. An analysis of clusters and main components was used for studying the divergences. The effects of the populations were not significant for all the characters analyzed in isolation. The gathering of pequi mother trees shown by multivariate analysis did not correspond to the grouping established by the populations. Selection for breeding and/or preservation purposes should concentrate efforts on fewer populations with larger numbers of mother trees per population. Selection of mother trees with higher fruits and thicker pulp fruits among them, even by visual evaluations only, is recommend in sampling mother trees for breeding purposes.
DOI: 10.6084/m9.figshare.6968150.v1
title: STUDY OF MACHINING OF THERMAL RECTIFICATED Corymbia citriodora AND Pinus elliottii

description: ABSTRACT This study evaluated the influence of thermal rectification in machining Corymbia citriodora and Pinus elliottii. The heat treatment was carried out for 6 hours at temperatures of 100°C, 140°C, 160°C, 180°C and 200°C and evaluated the performance of machining in three different material cutting tools, polished and hard metal carbide with TiB2 coating. The cutting power and roughness Ra and Rt of the machined surfaces with three different tools were evaluated. The power has proven lower for Pinus elliottii species. The roughness was lower for Corymbia citriodora wood. The roughness Ra and Rt were lower for heat treatment temperature of 180°C.
DOI: 10.6084/m9.figshare.6968144
title: Use of Infrared Spectroscopy and Near Infrared Hyperspectral Images to Evaluate Effects of Different Chemical Agents on PET Bottle Surface

description: PET - poly (ethylene terephthalate) - bottle surfaces were exposed to chemical agents and evaluated with middle infrared spectroscopy (MIR) and near infrared hyperspectral imaging (HSI-NIR). Images (928-2.524 nm) were acquired at different times from bottle surfaces exposed to alkaline, acidic and ethanolic media. Savitzky-Golay smoothing (2nd order polynomial and 5-point window) combined with Standard Normal Variate (SNV) was used for pre-processing data. Scores from principal component analysis (PCA) on images showed discrimination of PET according to exposure time, particularly when employing NaOH. After the first two hours of chemical exposure, significant contribution of the (-C-O + OH) combination band indicated formation of glycol ethylene on the PET surface, which was confirmed from PCA loading plots. MIR reflectance spectra ranged from 4.000 to 650 cm-1. Changes in spectral intensities could be associated with depolymerization of PET when exposed to NaOH. These were confirmed with PCA and attributed to the carboxylate group of terephthalate.
DOI: 10.6084/m9.figshare.6968147.v1
title: GENETIC DIVERGENCE IN POPULATIONS OF Caryocar brasiliense Camb. FROM THE PHYSICAL CHARACTERISTICS OF THE FRUITS

description: ABSTRACT The study of the genetic and phenotypic variability of the populations of Caryocar brasiliense Camb. assists in strategies for conservation and breeding of the specie. The objective of this work was to characterize the genetic diversity through the physical characteristics of pequi fruits of two populations through uni and multivariate analysis, as well as the implications in their domestication and breeding. Fruits were harvested from 20 mother trees (ten fruits per mother tree) from two populations in the state of Minas Gerais (Curvelo and São Gonçalo do Rio Preto Municipalities) during the years 2010, 2011 and 2012. The data were submitted to analysis of variance using a hierarchical classification model. An analysis of clusters and main components was used for studying the divergences. The effects of the populations were not significant for all the characters analyzed in isolation. The gathering of pequi mother trees shown by multivariate analysis did not correspond to the grouping established by the populations. Selection for breeding and/or preservation purposes should concentrate efforts on fewer populations with larger numbers of mother trees per population. Selection of mother trees with higher fruits and thicker pulp fruits among them, even by visual evaluations only, is recommend in sampling mother trees for breeding purposes.
DOI: 10.6084/m9.figshare.6968141
title: IRF6 rs2235375 single nucleotide polymorphism is associated with isolated non-syndromic cleft palate but not with cleft lip with or without palate in South Indian population

description: Abstract Introduction Transcription factors are very diverse family of proteins involved in activating or repressing the transcription of a gene at a given time. Several studies using animal models demonstrated the role of transcription factor genes in craniofacial development. Objective We aimed to investigate the association of IRF6 intron-6 polymorphism in the non-syndromic cleft lip with or without palate in a South Indian population. Methods 173 unrelated nonsyndromic cleft lip with or without cleft palate patients and 176 controls without clefts patients were genotyped for IRF6 rs2235375 variant by allele-specific amplification using the KASPar single nucleotide polymorphism genotyping system. The association between interferon regulatory factor-6 gene intron-6 dbSNP208032210:g.G&gt;C (rs2235375) single nucleotide polymorphism and non-syndromic cleft lip with or without palate risk was investigated by chi-square test. Results There were significant differences in genotype or allele frequencies of rs2235375 single nucleotide polymorphism between controls and cases with non-syndromic cleft lip with or without palate. IRF6 rs2235375 variant was significantly associated with increased risk of non-syndromic cleft lip with or without palate in co-dominant, dominant (OR: 1.19; 95% CI 1.03-2.51; p = 0.034) and allelic models (OR: 1.40; 95% CI 1.04-1.90; p = 0.028). When subset analysis was applied significantly increased risk was observed in cleft palate only group (OR dominant: 4.33; 95% CI 1.44-12.97; p = 0.005). Conclusion These results suggest that IRF6 rs2235375 SNP play a major role in the pathogenesis and risk of developing non-syndromic cleft lip with or without palate.