DOI: 10.5061/dryad.xgxd254s0
title: RNA-sequencing dataset of land snails collected in Australia

description: RNA-seq data of snail specimens (n=10) collected in Cairns QLD, Darwin NT
 and Adelaide SA,  targeting three main ports where introduced
 snails could have come in. RNA extractions were performed on all snail
 specimens using the modified protocol of Maxwell ® RSC simplyRNA Tissue
 Kit (Promega A1340) kit and the Maxwell ® RSC Instrument.
description: RNA-Seq data were generated from <strong>snail
 specimens</strong> (<em>n</em> = 10) collected in
 <strong>Cairns (QLD), Darwin (NT), and Adelaide (SA)</strong>,
 targeting three major ports where introduced snails may have entered.
 <strong>RNA extractions</strong> were performed using the
 <strong>Maxwell® RSC simplyRNA Tissue Kit (Promega
 A1340)</strong> and the <strong>Maxwell® RSC
 Instrument</strong>. <strong>Sequencing</strong> was
 conducted at the <strong>Australian Genome Research Facility Ltd.
 (AGRF Ltd.)</strong> using the <strong>Illumina NovaSeq X
 platform</strong>. A <strong>300-cycle run
 configuration</strong> was used for all samples, except
 <strong>Library36</strong>, which was sequenced with a
 <strong>150-cycle run configuration</strong>.
 <strong>Library preparation</strong> was performed using the
 <strong>Stranded Total RNA with Ribo-Zero Plus Library Prep
 Kit</strong>. <strong>Raw sequencing reads are included in
 this dataset.</strong>
description: # RNA-seq dataset of land snails collected in Australia
 [https://doi.org/10.5061/dryad.xgxd254s0](https://doi.org/10.5061/dryad.xgxd254s0) #### Description of the data and file structure **Principal Investigator Contact Information** Name: Dr. Valerie Caron Institution: CSIRO Email: [valerie.caron.enviro@gmail.com](mailto:valerie.caron.enviro@gmail.com) Name: Mariana Hopper Institution: CSIRO Email: [mariana.hopper@csiro.au](mailto:mariana.hopper@csiro.au) Alternate Contact Information Name: Berenice Talamantes Becerra Institution: CSIRO Email: [Berenice.TalamantesBecerra@csiro.au](mailto:Berenice.TalamantesBecerra@csiro.au) #### Dataset Overview This dataset comprises **RNA-Seq data** collected from **land snails in Australia**. The primary objective of this study was to **analyse the transcriptome profiles of invasive land snails** to investigate their potential role as **vectors**. **Live snails** were collected from **strategic locations** in the Australian port cities of **Darwin, Cairns, and Adelaide**, targeting **three key ports** where introduced snails may have arrived. **Species identification** was confirmed by **Richard Willan** from the **Museum and Art Gallery of the Northern Territory**. **Snails were kept alive** until they were stored at **-80ºC** for preservation. The dataset includes: * **Raw RNA-Seq data** in **FASTQ format** from whole snails collected in **Australian port cities** during **2024**. * **Assembled transcripts** for each library, which can be used for **further downstream analyses**. The **file names, snail species, and sampling locations** are detailed below. | **File name** | **State** | **Latitude** | **Longitude** | **Species Collected** | **Native or Introduced** | | :----------------------------------------------------------------------------- | :-------- | :----------- | :------------ | :---------------------- | :----------------------- | | Library1\_22NT3KLT3\_CCACGCTGAA-TATTCCTCAG\_L004\_R1.fastq.gz | QLD | -16.902124 | 145.71036 | *Leptopoma perlucida* | Native | | Library1\_22NT3KLT3\_CCACGCTGAA-TATTCCTCAG\_L004\_R2.fastq.gz | QLD | -16.902124 | 145.71036 | *Leptopoma perlucida* | Native | | Library1\_22NT3KLT3\_CCACGCTGAA-TATTCCTCAG\_L004\_assembly\_transcripts.fasta | QLD | -16.902124 | 145.71036 | *Leptopoma perlucida* | Native | | Library3\_22NT3KLT3\_ATGTCGTATT-TTCTTGCTGG\_L004\_R1.fastq.gz | QLD | -16.881993 | 145.710589 | *Paropeas achatinaceum* | Introduced | | Library3\_22NT3KLT3\_ATGTCGTATT-TTCTTGCTGG\_L004\_R2.fastq.gz | QLD | -16.881993 | 145.710589 | *Paropeas achatinaceum* | Introduced | | Library3\_22NT3KLT3\_ATGTCGTATT-TTCTTGCTGG\_L004\_assembly\_transcripts.fasta | QLD | -16.881993 | 145.710589 | *Paropeas achatinaceum* | Introduced | | Library4\_22NT3KLT3\_GCAATATTCA-GGCGCCAATT\_L004\_R1.fastq.gz | QLD | -16.902124 | 145.71036 | *Coneuplecta pampini* | Native | | Library4\_22NT3KLT3\_GCAATATTCA-GGCGCCAATT\_L004\_R2.fastq.gz | QLD | -16.902124 | 145.71036 | *Coneuplecta pampini* | Native | | Library4\_22NT3KLT3\_GCAATATTCA-GGCGCCAATT\_L004\_assembly\_transcripts.fasta | QLD | -16.902124 | 145.71036 | *Coneuplecta pampini* | Native | | Library6\_22NT3KLT3\_CTAGATTGCG-AGATATGGCG\_L004\_R1.fastq.gz | SA | -34.926163 | 138.495329 | *Theba pisana* | Introduced | | Library6\_22NT3KLT3\_CTAGATTGCG-AGATATGGCG\_L004\_R2.fastq.gz | SA | -34.926163 | 138.495329 | *Theba pisana* | Introduced | | Library6\_22NT3KLT3\_CTAGATTGCG-AGATATGGCG\_L004\_assembly\_transcripts.fasta | SA | -34.926163 | 138.495329 | *Theba pisana* | Introduced | | Library7\_22NT3KLT3\_CGATGCGGTT-CCTGCTTGGT\_L004\_R1.fastq.gz | SA | -34.926163 | 138.495329 | *Cochlicella acuta* | Introduced | | Library7\_22NT3KLT3\_CGATGCGGTT-CCTGCTTGGT\_L004\_R2.fastq.gz | SA | -34.926163 | 138.495329 | *Cochlicella acuta* | Introduced | | Library7\_22NT3KLT3\_CGATGCGGTT-CCTGCTTGGT\_L004\_assembly\_transcripts.fasta | SA | -34.926163 | 138.495329 | *Cochlicella acuta* | Introduced | | Library10\_22NT3KLT3\_AATTCCATCT-CTTCAGTTAC\_L004\_R1.fastq.gz | SA | -34.780746 | 138.481011 | *Cernuella virgata* | Introduced | | Library10\_22NT3KLT3\_AATTCCATCT-CTTCAGTTAC\_L004\_R2.fastq.gz | SA | -34.780746 | 138.481011 | *Cernuella virgata* | Introduced | | Library10\_22NT3KLT3\_AATTCCATCT-CTTCAGTTAC\_L004\_assembly\_transcripts.fasta | SA | -34.780746 | 138.481011 | *Cernuella virgata* | Introduced | | Library18\_22NT3KLT3\_TTAACCTTCG-AGGCCAGACA\_L004\_R1.fastq.gz | NT | -12.432255 | 130.935729 | *Allopeas gracile* | Introduced | | Library18\_22NT3KLT3\_TTAACCTTCG-AGGCCAGACA\_L004\_R2.fastq.gz | NT | -12.432255 | 130.935729 | *Allopeas gracile* | Introduced | | Library18\_22NT3KLT3\_TTAACCTTCG-AGGCCAGACA\_L004\_assembly\_transcripts.fasta | NT | -12.432255 | 130.935729 | *Allopeas gracile* | Introduced | | Library19\_22NT3KLT3\_CATATGCGAT-CCTTGAACGG\_L004\_R1.fastq.gz | NT | -12.470201 | 130.991047 | *Allopeas gracile* | Introduced | | Library19\_22NT3KLT3\_CATATGCGAT-CCTTGAACGG\_L004\_R2.fastq.gz | NT | -12.470201 | 130.991047 | *Allopeas gracile* | Introduced | | Library19\_22NT3KLT3\_CATATGCGAT-CCTTGAACGG\_L004\_assembly\_transcripts.fasta | NT | -12.470201 | 130.991047 | *Allopeas gracile* | Introduced | | Library20\_22NT3KLT3\_AGCCTATGAT-CACCACCTAC\_L004\_R1.fastq.gz | NT | -12.526667 | 131.153172 | *Gulella bicolor* | Introduced | | Library20\_22NT3KLT3\_AGCCTATGAT-CACCACCTAC\_L004\_R2.fastq.gz | NT | -12.526667 | 131.153172 | *Gulella bicolor* | Introduced | | Library20\_22NT3KLT3\_AGCCTATGAT-CACCACCTAC\_L004\_assembly\_transcripts.fasta | NT | -12.526667 | 131.153172 | *Gulella bicolor* | Introduced | | Library36\_L001\_L002\_L003\_L004\_L005\_L006\_L007\_L008\_R1.fastq.gz | SA | -34.899758 | 138.48827 | *Cochlicella sp.* | Introduced | | Library36\_L001\_L002\_L003\_L004\_L005\_L006\_L007\_L008\_R2.fastq.gz | SA | -34.899758 | 138.48827 | *Cochlicella sp.* | Introduced | | Library36\_L001\_L002\_L003\_L004\_L005\_L006\_L007\_L008\_transcripts.fasta | SA | -34.899758 | 138.48827 | *Cochlicella sp.* | Introduced | #### Funding This project was funded by the Department of Agriculture, Fisheries and Forestry, Australian Government #### Ethics Approval Not applicable #### Raw Data Files RNA sequencing was conducted by the **Australian Genome Research Facility Ltd. (AGRF Ltd.)** using the **Illumina NovaSeq X platform**. A **300-cycle paired-end run configuration** was used for **Libraries 1 to 20**, while **Library 36** was sequenced with a **150-cycle paired-end configuration**. Library preparation was performed using the **Stranded Total RNA with Ribo-Zero Plus Library Prep Kit**. On average, sequencing generated **at least 56 million reads per sample**. Library1_22NT3KLT3_CCACGCTGAA-TATTCCTCAG_L004_R1.fastq.gz Library1_22NT3KLT3_CCACGCTGAA-TATTCCTCAG_L004_R2.fastq.gz Library3_22NT3KLT3_ATGTCGTATT-TTCTTGCTGG_L004_R1.fastq.gz Library3_22NT3KLT3_ATGTCGTATT-TTCTTGCTGG_L004_R2.fastq.gz Library4_22NT3KLT3_GCAATATTCA-GGCGCCAATT_L004_R1.fastq.gz Library4_22NT3KLT3_GCAATATTCA-GGCGCCAATT_L004_R2.fastq.gz Library6_22NT3KLT3_CTAGATTGCG-AGATATGGCG_L004_R1.fastq.gz Library6_22NT3KLT3_CTAGATTGCG-AGATATGGCG_L004_R2.fastq.gz Library7_22NT3KLT3_CGATGCGGTT-CCTGCTTGGT_L004_R1.fastq.gz Library7_22NT3KLT3_CGATGCGGTT-CCTGCTTGGT_L004_R2.fastq.gz Library10_22NT3KLT3_AATTCCATCT-CTTCAGTTAC_L004_R1.fastq.gz Library10_22NT3KLT3_AATTCCATCT-CTTCAGTTAC_L004_R2.fastq.gz Library18_22NT3KLT3_TTAACCTTCG-AGGCCAGACA_L004_R1.fastq.gz Library18_22NT3KLT3_TTAACCTTCG-AGGCCAGACA_L004_R2.fastq.gz Library19_22NT3KLT3_CATATGCGAT-CCTTGAACGG_L004_R1.fastq.gz Library19_22NT3KLT3_CATATGCGAT-CCTTGAACGG_L004_R2.fastq.gz Library20_22NT3KLT3_AGCCTATGAT-CACCACCTAC_L004_R1.fastq.gz Library20_22NT3KLT3_AGCCTATGAT-CACCACCTAC_L004_R2.fastq.gz Library36_L001_L002_L003_L004_L005_L006_L007_L008_R1.fastq.gz Library36_L001_L002_L003_L004_L005_L006_L007_L008_R2.fastq.gz ### All Files and Variables #### File: Library1\_22NT3KLT3\_CCACGCTGAA-TATTCCTCAG\_L004\_R1.fastq.gz **Description:** RNA data of *Leptopoma perlucida* R1 for metagenomic analyses #### File: Library1\_22NT3KLT3\_CCACGCTGAA-TATTCCTCAG\_L004\_R2.fastq.gz **Description:**  RNA data of *Leptopoma perlucida* R2 for metagenomic analyses #### File: Library1\_22NT3KLT3\_CCACGCTGAA-TATTCCTCAG\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts of *Leptopoma perlucida*  #### File: Library3\_22NT3KLT3\_ATGTCGTATT-TTCTTGCTGG\_L004\_R1.fastq.gz **Description:** RNA data of *Paropeas achatinaceum* R1 for metagenomic analyses #### File: Library3\_22NT3KLT3\_ATGTCGTATT-TTCTTGCTGG\_L004\_R2.fastq.gz **Description:** RNA data of *Paropeas achatinaceum* R2 for metagenomic analyses #### File: Library3\_22NT3KLT3\_ATGTCGTATT-TTCTTGCTGG\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts of *Paropeas achatinaceum* #### File: Library4\_22NT3KLT3\_GCAATATTCA-GGCGCCAATT\_L004\_R1.fastq.gz **Description:** RNA data of *Coneuplecta pampini* R1 for metagenomic analyses #### File: Library4\_22NT3KLT3\_GCAATATTCA-GGCGCCAATT\_L004\_R2.fastq.gz **Description:** RNA data of *Coneuplecta pampini* R2 for metagenomic analyses #### File: Library4\_22NT3KLT3\_GCAATATTCA-GGCGCCAATT\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts of *Coneuplecta pampini* #### File: Library6\_22NT3KLT3\_CTAGATTGCG-AGATATGGCG\_L004\_R1.fastq.gz **Description:** RNA data of *Theba pisana* R1 for metagenomic analyses #### File: Library6\_22NT3KLT3\_CTAGATTGCG-AGATATGGCG\_L004\_R2.fastq.gz **Description:** RNA data of *Theba pisana* R2 for metagenomic analyses #### File: Library6\_22NT3KLT3\_CTAGATTGCG-AGATATGGCG\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts *Theba pisana* #### File: Library7\_22NT3KLT3\_CGATGCGGTT-CCTGCTTGGT\_L004\_R1.fastq.gz **Description:** RNA data of *Cochlicella acuta* R1 for metagenomic analyses #### File: Library7\_22NT3KLT3\_CGATGCGGTT-CCTGCTTGGT\_L004\_R2.fastq.gz **Description:** RNA data of *Cochlicella acuta* R2 for metagenomic analyses #### File: Library7\_22NT3KLT3\_CGATGCGGTT-CCTGCTTGGT\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts *Cochlicella acuta* #### File: Library10\_22NT3KLT3\_AATTCCATCT-CTTCAGTTAC\_L004\_R1.fastq.gz **Description:** RNA data of *Cernuella virgata* R1 for metagenomic analyses #### File: Library10\_22NT3KLT3\_AATTCCATCT-CTTCAGTTAC\_L004\_R2.fastq.gz **Description:** RNA data of *Cernuella virgata* R2 for metagenomic analyses #### File: Library10\_22NT3KLT3\_AATTCCATCT-CTTCAGTTAC\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts *Cernuella virgata* #### File: Library18\_22NT3KLT3\_TTAACCTTCG-AGGCCAGACA\_L004\_R1.fastq.gz **Description:** RNA data of *Allopeas gracile* R1 for metagenomic analyses #### File: Library18\_22NT3KLT3\_TTAACCTTCG-AGGCCAGACA\_L004\_R2.fastq.gz **Description:** RNA data of **Allopeas gracile**R2 for metagenomic analyses #### File: Library18\_22NT3KLT3\_TTAACCTTCG-AGGCCAGACA\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts *Allopeas gracile* #### File: Library19\_22NT3KLT3\_CATATGCGAT-CCTTGAACGG\_L004\_R1.fastq.gz **Description:** RNA data of **Allopeas gracile**R1 for metagenomic analyses #### File: Library19\_22NT3KLT3\_CATATGCGAT-CCTTGAACGG\_L004\_R2.fastq.gz **Description:** RNA data of *Allopeas gracile* R2 for metagenomic analyses #### File: Library19\_22NT3KLT3\_CATATGCGAT-CCTTGAACGG\_assembly\_transcripts.fasta **Description:** Assembled transcripts *Allopeas gracile* #### File: Library20\_22NT3KLT3\_AGCCTATGAT-CACCACCTAC\_L004\_R1.fastq.gz **Description:** RNA data of *Gulella bicolor* R1 for metagenomic analyses #### File: Library20\_22NT3KLT3\_AGCCTATGAT-CACCACCTAC\_L004\_R2.fastq.gz **Description:** RNA data of *Gulella bicolor* R2 for metagenomic analyses #### File: Library20\_22NT3KLT3\_AGCCTATGAT-CACCACCTAC\_L004\_assembly\_transcripts.fasta **Description:** Assembled transcripts *Gulella bicolor* #### File: Library36\_L001\_L002\_L003\_L004\_L005\_L006\_L007\_L008\_R1.fastq.gz **Description:** RNA data of Cochlicella sp. R1 for metagenomic analyses #### File: Library36\_L001\_L002\_L003\_L004\_L005\_L006\_L007\_L008\_R2.fastq.gz **Description:** RNA data of Cochlicella sp. R2 for metagenomic analyses #### File: Library36\_L001\_L002\_L003\_L004\_L005\_L006\_L007\_L008\_transcripts.fasta **Description:** Assembled transcripts *Cochlicella sp.* ## Code/software The sequencing provider delivered the data as **raw FASTQ files**, which are being uploaded **in their original format**. ### **Tools Used for Transcriptome Assembly** #### **1. Quality Control (QC) and Adapter Removal** QC and adapter trimming were performed using **Trim Galore!** (v0.6.4) with default parameters: 🔗 [Trim Galore! GitHub](https://github.com/FelixKrueger/TrimGalore/) **Command:** ``` trim_galore --paired --fastqc --output_dir path/to/output path/R1 path/R2 ``` #### **2. RNA-Seq Assembly** Transcriptome assembly was performed using **SPAdes (v4.0.0)** with default parameters for RNA-Seq: 🔗 [SPAdes GitHub](https://github.com/ablab/spades) **Command:** ``` spades.py --tmp-dir path/tmp --rna -1 path/R1 -2 path/R2 -o path/output ``` Users can employ **other bioinformatics tools** to visualize the data, using **Illumina paired-end reads** as input. ## Access information The dataset includes RNA sequencing results from terrestrial snails collected across Australia. The number of samples and species collected was limited to sampling sites and species found. This dataset comprises genetic information derived from native and introduced Australian biodiversity. Users are advised that the data is provided for non-commercial research use. Any commercial use or bioprospecting of the data requires prior informed consent and mutually agreed terms with CSIRO. Other publicly accessible locations of the data: * Not applicable Data was derived from the following sources: * Not applicable