cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5668357.v1
title: Circulating levels of adiponectin and extent of coronary artery disease in patients undergoing elective coronary angiography

description: Adiponectin (APN), an adipose tissue-released adipokine with demonstrated anti-inflammatory and anti-atherogenic properties, is encoded by a gene whose polymorphisms are associated with presence of coronary artery disease (CAD). Serum APN levels are inversely related with presence and complexity of CAD. Within this context, we sought to compare levels of total APN and its high molecular weight form (HMW APN) according to clinical presentation and extent of CAD in patients undergoing elective cardiac catheterization. From March 2008 to June 2010, clinical data and blood samples for APN and HMW APN measurements were collected from 415 subjects undergoing cardiac catheterization at two tertiary centers. CAD extent was estimated by the number of coronary arteries with significant stenosis (≥70% obstruction in a major coronary artery) and by Duke Jeopardy Score (DJS). Serum APN levels were similar between groups with stable or unstable CAD (APN 9.20±5.88 vs 9.47±6.23 μg/mL, P=0.738, and HMW APN 5.31±3.72 vs 5.91±4.16 μg/mL, P=0.255), even after stratification by the number of arteries involved (single-vessel vs multivessel disease: APN 9.39±5.76 vs 9.26±6.27 μg/mL, P=0.871; HMW APN 5.29±3.79 vs 5.83±4.04 μg/mL, P=0.306) and DJS score (APN, P=0.718; HMW APN, P=0.276). We conclude that APN and HMW APN serum levels are similar across clinical presentations and different extents of CAD, despite being significantly lower in the presence of obstructive CAD.
DOI: 10.6084/m9.figshare.5668354
title: Analysis of agreement between peak expiratory flow meters and comparison of reference values

description: Abstract Introduction: The peak expiratory flow (PEF) is a practical method that evaluates the caliber of the proximal airways. The ease in handling and the low cost of portable PEF meters made their use frequent. However, few studies have examined the correlation between the different equipment and compared their reference values. Objective: Analyze the correlation of three portable meters of PEF and check if the reference values are applicable in the studied population. Methods: 168 healthy individuals, of both genders, ranging in age from 20 to 80 years, had their PEF measured in Mini-Wright®, Assess® and AirZone® and the values obtained were compared to those predicted by Leiner et al. (1963) and Nunn and Gregg (1989). Statistical analysis was performed by the Kolmogorov-Smirnov tests, ANOVA, Wilcoxon test, Spearman correlation and analysis of agreement of Bland-Altman (p&lt; 0.05). Results: There was significant difference in the values of PEF obtained between the Mini-Wright® and Assess® meters in both genders, between the AirZone® and Assess® only in men and between the Mini-Wright® and AirZone® only in women. The predicted values in the three meters have overestimated the obtained in both genders; there was no correlation between the values obtained from three meters due to the great variation of agreement limits and large interindividual variation. Conclusion: The values obtained in the three meters are not interchangeable and the predicted values were unsuitable for the sample.
DOI: 10.6084/m9.figshare.5668354.v1
title: Analysis of agreement between peak expiratory flow meters and comparison of reference values

description: Abstract Introduction: The peak expiratory flow (PEF) is a practical method that evaluates the caliber of the proximal airways. The ease in handling and the low cost of portable PEF meters made their use frequent. However, few studies have examined the correlation between the different equipment and compared their reference values. Objective: Analyze the correlation of three portable meters of PEF and check if the reference values are applicable in the studied population. Methods: 168 healthy individuals, of both genders, ranging in age from 20 to 80 years, had their PEF measured in Mini-Wright®, Assess® and AirZone® and the values obtained were compared to those predicted by Leiner et al. (1963) and Nunn and Gregg (1989). Statistical analysis was performed by the Kolmogorov-Smirnov tests, ANOVA, Wilcoxon test, Spearman correlation and analysis of agreement of Bland-Altman (p&lt; 0.05). Results: There was significant difference in the values of PEF obtained between the Mini-Wright® and Assess® meters in both genders, between the AirZone® and Assess® only in men and between the Mini-Wright® and AirZone® only in women. The predicted values in the three meters have overestimated the obtained in both genders; there was no correlation between the values obtained from three meters due to the great variation of agreement limits and large interindividual variation. Conclusion: The values obtained in the three meters are not interchangeable and the predicted values were unsuitable for the sample.
DOI: 10.6084/m9.figshare.5668351
title: Agronomic and P recovery efficiency of organomineral phosphate fertilizer from poultry litter in sandy and clayey soils

description: Abstract: The objective of this work was to evaluate agronomic and P recovery efficiencies of a granulated organomineral phosphate fertilizer (OMF) produced from poultry litter, compared with those of monoammonium phosphate (MAP), in soils with different textures. The experiment followed a 2x2x4+2 factorial arrangement, with two Oxisols (sandy loam and clay loam textures), two sources of P (OMF and MAP), four levels of P (50, 100, 200, and 400 mg kg-1), besides two control treatments without P application. The treatments were evaluated in 10-kg pots filled with soil, during four successive cultivations of corn without replacing the P absorbed by the plants, in order to evaluate the actual and residual effects of the fertilizers. Available P contents in the soil were higher with the MAP fertilizer in the sandy loam soil, with no significant differences between fertilizers in the clay loam soil. OMF had a greater immediate effect and promoted a higher dry matter yield in the first cultivation; however, the residual effects of the fertilizers did not differ in the other cultivations. P accumulation by plants was not affected by the fertilizer used. OMF had higher agronomic efficiency, but P recovery efficiency did not differ significantly between fertilizers. OMF performance indicates good potential use of poultry litter as fertilizer, in organomineral formulations.
DOI: 10.6084/m9.figshare.5668351.v1
title: Agronomic and P recovery efficiency of organomineral phosphate fertilizer from poultry litter in sandy and clayey soils

description: Abstract: The objective of this work was to evaluate agronomic and P recovery efficiencies of a granulated organomineral phosphate fertilizer (OMF) produced from poultry litter, compared with those of monoammonium phosphate (MAP), in soils with different textures. The experiment followed a 2x2x4+2 factorial arrangement, with two Oxisols (sandy loam and clay loam textures), two sources of P (OMF and MAP), four levels of P (50, 100, 200, and 400 mg kg-1), besides two control treatments without P application. The treatments were evaluated in 10-kg pots filled with soil, during four successive cultivations of corn without replacing the P absorbed by the plants, in order to evaluate the actual and residual effects of the fertilizers. Available P contents in the soil were higher with the MAP fertilizer in the sandy loam soil, with no significant differences between fertilizers in the clay loam soil. OMF had a greater immediate effect and promoted a higher dry matter yield in the first cultivation; however, the residual effects of the fertilizers did not differ in the other cultivations. P accumulation by plants was not affected by the fertilizer used. OMF had higher agronomic efficiency, but P recovery efficiency did not differ significantly between fertilizers. OMF performance indicates good potential use of poultry litter as fertilizer, in organomineral formulations.
DOI: 10.6084/m9.figshare.5668348
title: Plant growth regulation: a method for fine-tuning mepiquat chloride rates in cotton1

description: ABSTRACT Plant growth regulator management has been a challenge for cotton growers, due to the increased weather instability and the adoption of early cultivars, in off-season crops. This study aimed at proposing a new method, called Crop Growth Rate, to define mepiquat chloride rates in cotton crops, as well as to compare it with traditional management practices. Except for micronaire, there was no difference in cotton growth parameters, neither in yield nor fiber quality, between the new method and the traditional one. The mepiquat chloride rates were, on average, 24 % higher and 43 % lower in late and early maturity cultivars, respectively, for the new method. The number of bolls in plants treated with mepiquat chloride was lower than in non-treated plants, for the IMA5672B2RF and IMA5675B2RF cultivars, but a higher average weight of bolls was observed for these genotypes. The new method is efficient in defining the plant growth regulator rate to avoid an excessive growth and results in less mepiquat chloride applied to early cycle cultivars, preserving lint yield and fiber quality.
DOI: 10.6084/m9.figshare.5668348.v1
title: Plant growth regulation: a method for fine-tuning mepiquat chloride rates in cotton1

description: ABSTRACT Plant growth regulator management has been a challenge for cotton growers, due to the increased weather instability and the adoption of early cultivars, in off-season crops. This study aimed at proposing a new method, called Crop Growth Rate, to define mepiquat chloride rates in cotton crops, as well as to compare it with traditional management practices. Except for micronaire, there was no difference in cotton growth parameters, neither in yield nor fiber quality, between the new method and the traditional one. The mepiquat chloride rates were, on average, 24 % higher and 43 % lower in late and early maturity cultivars, respectively, for the new method. The number of bolls in plants treated with mepiquat chloride was lower than in non-treated plants, for the IMA5672B2RF and IMA5675B2RF cultivars, but a higher average weight of bolls was observed for these genotypes. The new method is efficient in defining the plant growth regulator rate to avoid an excessive growth and results in less mepiquat chloride applied to early cycle cultivars, preserving lint yield and fiber quality.
DOI: 10.6084/m9.figshare.5668345
title: Anatomic, tomographic and sonographic study of podotrochlear apparatus of adult horses

description: ABSTRACT The navicular syndrome is a condition involving the podotrochlear apparatus and represents one of the most common causes of forelimb lameness in horses. Therefore, further study of this region is of interest when it comes to diagnosis and treatment of lameness in horses. The aim of this study was to demonstrate the differences between the imaging findings of ultrasonography and computed tomography in the evaluation of the palmar structures of the podotrochlear apparatus of healthy adult horses and description of the structures observed in images obtained with these techniques. For this, four images of four anatomical parts were performed, and subsequently sectioned and used for the anatomical description. The use of anatomical parts helps in the understanding of normal anatomy leading to a better interpretation of the images and increasing the specificity of the diagnostic for detecting changes that cause diseases. Ultrasonography provides relevant information about these structures to be studied and the association with computed tomography (CT) increased the accuracy of the investigation. Despite the use of CT being more suitable for bone tissue it provides important information and can be used as a useful tool when there is no available MRI.
DOI: 10.6084/m9.figshare.5668345.v1
title: Anatomic, tomographic and sonographic study of podotrochlear apparatus of adult horses

description: ABSTRACT The navicular syndrome is a condition involving the podotrochlear apparatus and represents one of the most common causes of forelimb lameness in horses. Therefore, further study of this region is of interest when it comes to diagnosis and treatment of lameness in horses. The aim of this study was to demonstrate the differences between the imaging findings of ultrasonography and computed tomography in the evaluation of the palmar structures of the podotrochlear apparatus of healthy adult horses and description of the structures observed in images obtained with these techniques. For this, four images of four anatomical parts were performed, and subsequently sectioned and used for the anatomical description. The use of anatomical parts helps in the understanding of normal anatomy leading to a better interpretation of the images and increasing the specificity of the diagnostic for detecting changes that cause diseases. Ultrasonography provides relevant information about these structures to be studied and the association with computed tomography (CT) increased the accuracy of the investigation. Despite the use of CT being more suitable for bone tissue it provides important information and can be used as a useful tool when there is no available MRI.
DOI: 10.6084/m9.figshare.5668342
title: Handgrip and functional capacity in Chronic Obstructive Pulmonary Disease patients

description: Abstract Introduction: In the Chronic Obstructive Pulmonary Disease (COPD) both pulmonary and systemic condition increase dyspnea, intolerance to exercise and inactivity. Objective: To evaluate possible association between Hand Grip Strength (HGS) and the distance covered in the Six-Minute Walk Test (6MWT) in patients with Chronic Obstructive Pulmonary Disease (COPD). Methods: A cross-sectional study that evaluated 34 patients with COPD from moderate to very severe stages. The HGS test was performed with a manual hydraulic dynamometer (Jamar®, California, USA) with three bilateral measures, allowing 60-seconds rest time in-between measurements. Afterwards, the patients were submitted to the 6MWT along a 30-meters level corridor following the rules of the American Thoracic Society. Results: Average age 62.7±7.2 years old, body mass index 26.7±6.9 Kg/m². The value of the HGS in the dominant hand was 28.0±8.7 Kgf and in the non-dominant hand was 26.7±7.7 Kgf (99.2% of the predicted value and 106.3% of the predicted value, respectively). The subjects covered in average 421.0±110.4 meters in the 6MWT and there has been detected direct and significant correlation between the distance covered and the HGS of the dominant hand (r=0,430; p=0,011) and non-dominant (r=0,502; p=0,002). The patients with COPD that presented lower HGS also covered less distance in the 6MWT. Conclusion: The hand grip strength was directly associated with the functional capacity assessed through the distance covered in the Six-Minute Walk Test in the evaluated trial.
DOI: 10.6084/m9.figshare.5668339
title: Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region

description: The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P&lt;0.01). Despite the lack of individual significant differences, PTGS2 PR constructs enclosing rs689466 G (pAGG and pAGC) showed an approximate two-fold increase in luciferase activity when compared to those containing rs689466 A (pAAG, pGAC, pAAC and pGAG) in both cell lines (P&lt;0.001 for MCF-7 and P=0.03 for HEK293FT). The effect of PTGS2 3′-UTR sequences varied between MCF-7 and HEK293FT: MCF-7 cells showed significant reduction (40–60%) in luciferase activity (at least P&lt;0.01), whereas HEK293FT cells showed more diverse results, with an average 2-fold increase when combined constructs (PR and 3′-UTR) were compared to respective parental PR sequences. The contribution of 3′-UTR variant (rs5275) was not consistent in either cell line. Despite the modulation of the 3′-UTR, with variable effects of rs5275, the enhancing transcriptional effect of rs689466 G was still detectable (P&lt;0.0001 in MCF-7 or P=0.03 in HEK293FT cells).
DOI: 10.6084/m9.figshare.5668339.v1
title: Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region

description: The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P&lt;0.01). Despite the lack of individual significant differences, PTGS2 PR constructs enclosing rs689466 G (pAGG and pAGC) showed an approximate two-fold increase in luciferase activity when compared to those containing rs689466 A (pAAG, pGAC, pAAC and pGAG) in both cell lines (P&lt;0.001 for MCF-7 and P=0.03 for HEK293FT). The effect of PTGS2 3′-UTR sequences varied between MCF-7 and HEK293FT: MCF-7 cells showed significant reduction (40–60%) in luciferase activity (at least P&lt;0.01), whereas HEK293FT cells showed more diverse results, with an average 2-fold increase when combined constructs (PR and 3′-UTR) were compared to respective parental PR sequences. The contribution of 3′-UTR variant (rs5275) was not consistent in either cell line. Despite the modulation of the 3′-UTR, with variable effects of rs5275, the enhancing transcriptional effect of rs689466 G was still detectable (P&lt;0.0001 in MCF-7 or P=0.03 in HEK293FT cells).
DOI: 10.6084/m9.figshare.5668336
title: Cystic adventitial disease of the popliteal artery: an infrequent cause of intermittent claudication

description: Intermittent claudication is frequently associated with atherosclerotic disease, but differential diagnosis must be sought in patients with no traditional risk factors. Cystic adventitial disease, of unknown etiology, most frequently affects the popliteal artery, and occasionally presents as intermittent claudication. We report a case of this disease and the surgical treatment, and discuss some aspects related to etiopathogenesis, diagnosis and treatment of this condition.
DOI: 10.6084/m9.figshare.5668336.v1
title: Cystic adventitial disease of the popliteal artery: an infrequent cause of intermittent claudication

description: Intermittent claudication is frequently associated with atherosclerotic disease, but differential diagnosis must be sought in patients with no traditional risk factors. Cystic adventitial disease, of unknown etiology, most frequently affects the popliteal artery, and occasionally presents as intermittent claudication. We report a case of this disease and the surgical treatment, and discuss some aspects related to etiopathogenesis, diagnosis and treatment of this condition.
DOI: 10.6084/m9.figshare.5668333
title: Systemic inflammatory response syndrome criteria and the prediction of hospital mortality in critically ill patients: a retrospective cohort study

description: ABSTRACT Objective: This study intended to determine whether the systemic inflammatory response syndrome criteria can predict hospital mortality in a Brazilian cohort of critically ill patients. Methods: We performed a retrospective cohort study at a private tertiary hospital in São Paulo (SP), Brazil. We extracted information from the adult intensive care unit database (Sistema EpimedTM). We compared the SAPS 3 and the systemic inflammatory response syndrome model as dichotomous (≥ 2 criteria: systemic inflammatory response syndrome -positive versus 0 - 1 criterion: systemic inflammatory response syndrome -negative) and ordinal variables from 0 to 4 (according to the number of systemic inflammatory response syndrome criteria met) in the prediction of hospital mortality at intensive care unit admission. Model discrimination was compared using the area under the receiver operating characteristics (AUROC) curve. Results: From January to December 2012, we studied 932 patients (60.4% were systemic inflammatory response syndrome -positive). systemic inflammatory response syndrome -positive patients were more critically ill than systemic inflammatory response syndrome -negative patients and had higher hospital mortality (16.9% versus 8.1%, p &lt; 0.001). In the adjusted analysis, being systemic inflammatory response syndrome -positive independently increased the risk of death by 82% (odds ratio 1.82; 95% confidence interval [CI] 1.12 - 2.96, p = 0.016). However, the AUROC curve for the SAPS 3 model was higher (0.81, 95%CI 0.78 - 0.85) compared to the systemic inflammatory response syndrome model with the systemic inflammatory response syndrome criteria as a dichotomous variable (0.60, 95%CI 0.55 - 0.65) and as an ordinal variable (0.62, 95%CI 0.57 - 0.68; p &lt; 0.001) for hospital mortality. Conclusion: Although systemic inflammatory response syndrome is associated with hospital mortality, the systemic inflammatory response syndrome criteria show low accuracy in the prediction of mortality compared with the SAPS 3.
DOI: 10.6084/m9.figshare.5668330
title: Effects of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) release from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone

description: Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused to these microcapsules using the dichloromethane vapor method. The bioactivity of the released BMP-2 and VEGF was then quantified in rat mesenchymal stem cells (rMSCs). Immunoblotting analysis showed that BMP-2/PELA/VEG promoted the differentiation of rMSCs into osteoblasts via the MAPK and Wnt pathways. Osteoblast differentiation was assessed through alkaline phosphatase expression. When compared with simple BMP-2 plus VEGF group and pure PELA group, osteoblast differentiation in BMP-2/PELA/VEGF group significantly increased. An MTT assay indicated that BMP-2-loaded PELA scaffolds had no adverse effects on cell activity. BMP-2/PELA/VEG promoted the differentiation of rMSCs into osteoblast via the ERK1/2 and Wnt pathways. Our findings indicate that the sequential release of BMP-2 and VEGF from PELA microcapsule-based scaffolds is a promising approach for the treatment of bone defects.