DOI: 10.5061/dryad.p8cz8wb1d
title: Hawaiian caterpillar patrols spiderwebs camouflaged in insect prey’s body parts

description: Lepidoptera is the most herbivorous of all the insect orders with
 predatory caterpillars globally comprising less than .13% of the nearly
 200,000 moth and butterfly species. Here, we describe a species whose
 caterpillars are carnivorous inhabitants of spider’s webs, feeding on
 arthropods they find there. This Hawaiian lineage also boasts an
 unprecedented and macabre practice of decorating its portable larval home
 with the body parts of spider prey it harvests from the web where it
 resides. Phylogenomic data suggests the origin of this unique spider
 cohabitant is at least six million years old, over one million years older
 than Hawaii’s current high islands. After decades of searching, only one
 species has been discovered, restricted to 15 km2 on a single mountain
 range on the island of Oʻahu, meaning other members of the lineage have
 disappeared from older islands. Conservation action to save this globally
 unique lineage is imperative and overdue. 
description: # Hawaiian caterpillar patrols spiderwebs camouflaged in insect prey’s
 body parts
 [https://doi.org/10.5061/dryad.p8cz8wb1d](https://doi.org/10.5061/dryad.p8cz8wb1d) ## Description of the data and file structure This dataset contains the scripts used in the analyses by Rubinoff et al. (in review). These scripts were used to process raw Illumina WGS reads from species of Hyposmocoma to extract loci used in Kawahara et al. (2019) for phylogenomic analysis. Once loci were extracted, we aligned loci using MAFFT and inferred phylogenetic trees using IQtree.  Divergence time dating was conducted using MCMCtree in the PAML package.  ### Files and variables #### File: SM\_CSI.zip ### Description of the data and file structure This zip file contains scripts used to extract loci from raw WGS reads using hybpiper and conduct phylogenetic analysis using iqtree and MCMCtree. #### Files and Folders ##### Hybpiper/01\_clean\_hybpiper.slurm Script used to clean and run the assembly step of Hybpiper. ##### Hybpiper/02\_hybpiper\_stats.slurm Script used to calculate assembly stats for Hybpiper. ##### Hybpiper/03\_hybpiper\_extract\_sequences.slurm Script used to extract loci. ##### Hybpiper/Akito\_markers\_DPLEX.AA\_hybpip\_fixed.fa This file is the protein sequences from Kawahara et al (2019) from Danaus plexippus.  ##### Hybpiper/CSI\_mafft\_aligned/\* This folder contains the mafft aligned loci extracted from Hybpiper using the Akito_markers_DPLEX.AA_hybpip_fixed.fa as baits. ##### Hybpiper/CSI\_min\_75\_17/\* This folder contains the mafft aligned loci extracted from Hybpiper that passed filtering by sequence length and were present in all 17 Hyposmocoma samples.  ##### IQTREE/ This folder contains the dataset, partition files, and output files from the IQtree run. ###### CSI\_merge\_17\_partitions.txt This file contains the partitions used to conduct partition analyses in IQTREE. ###### CSI\_merge\_17\_partitions.txt.best\_model.nex This file contains the best models for each partition used, found in the model finder implemented in IQTREE ###### CSI_merge_17_partitions.txt.treefile This file contains the maximum likelihood tree found among the 10 runs of IQTREE.  ##### MCMCTREE/ This folder contains the dataset, MCMC_Treefile, and config files for MCMCtree. ###### CSI\_merge\_17.fasta This file contains the sequence alignment used in MCMCtree and IQTREE ###### CSI_merge_17_MCMCtree.treefile This file is the likelihood tree file from IQTREE used by MCMCtree for our dating analyses. ###### CSI_merge_17_MCMC_TREE_date.tre This file is the dated phylogeny inferred from MCMCtree. ###### mcmctree_config_step_1.ctl This file is the config file for step 1 in the MCMCtree analysis. ###### mcmctree_config_step_2.ctl This file is the config file for step 2 in the MCMCtree analysis. ## Access information Other publicly accessible locations of the data: * NCBI Genbank Bioproject PRJNA1143550. * Akito_markers_DPLEX.AA_hybpip_fixed.fa was derived from the following * [https://datadryad.org/stash/dataset/doi:10.5061/dryad.j477b40](https://datadryad.org/stash/dataset/doi:10.5061/dryad.j477b40)