DOI: 10.5061/dryad.sf7m0cgcn
title: Data from: Comparison of dopamine release and uptake parameters across sex, species and striatal subregions

description: Dopamine in the striatum strongly regulates behavioral
 output in a heterogenous across the
 various striatal subregions. Moreover, dopamine
 dynamics not only displays heterogeneity across brain structures but also
 within males and females. The purpose of this dataset was to evaluate the
 dopamine dynamics in male and female mice and rats across five subregions:
 the dorsolateral caudate, ventromedial caudate, nucleus accumbens core,
 nucleus accumbens lateral shell, and the nucleus accumbens medial shell.
 Fast scan cyclic voltammetry (FSCV) was employed to measure dopamine
 release and uptake following a single pulse electrical stimulation in each
 of these subregions within a single brain slice. The dopamine dynamics
 were also observed across a variety of stimulation amplitudes. The goal of
 this dataset was to produce systematic FSCV measurements of dopamine
 across the rodent striatum using FSCV which would be available as
 a resource for further investigation of DA terminal function.
description: Detailed methods can be found in the manuscript.
description: # Comparison of dopamine release and uptake parameters across sex, species
 and striatal subregions
 [https://doi.org/10.5061/dryad.sf7m0cgcn](https://doi.org/10.5061/dryad.sf7m0cgcn) ## Description of the data and file structure This data set includes 12 mice (6 male, 6 female) and 12 rats (6 male, 6 female). One rostral (mouse: 1.33-1.09 AP; rat: 2.20-1.60 AP) and one caudal (mouse: 0.97-0.73 AP, rat: 1.20-0.70 AP) brain slice from each animal hemisected so that a total of 4 data points were collected per animal. All slices were run in parallel, and an additional slice was collected from each animal to serve as a control for slice health and electrode stability throughout the recording period. Data identifiers have been designated as follows: Species (Ms= mouse or Rt=rat), Sex (M=male; F=female), animal number, slice identity (r=rostral, c=caudal), slice number. Example: MsM1_r1 would be mouse, male 1, rostral slice 1.  The study was designed to maximize the data collected, allowing for within subject comparison by measuring dopamine signals from 5 different brain regions within a single slice. The five brain regions examined were the dorsolateral caudate putamen (dlCPu), ventromedial caudate putamen (vmCPu), nucleus accumbens core (core), nucleus accumbens lateral shell (lshell), and nucleus accumbens medial shell (mshell). Placements within these regions were marked according to the atlas (Paxinos, 2013) once a stable dopamine baseline was collected at a stimulation amplitude of 0.75 mA with a 180 second interstimulus interval. The order in which these measurements were performed was randomized for each slice using randomization.com (seed 3016). The file names were coded according to placement number rather than region so that the researchers were blinded to region and animal during analysis. However, since the male and female cohorts were run and analyzed separately, the sex was not blinded during analysis. The files were only unblinded after they were analyzed and those included herein are unblinded.  Once a stable dopamine baseline was obtained, the stimulation amplitude was varied as follows: 0.14, 0.16, 0.18, 0.20, 0.22, 0.24, 0.26, 0.28, 0.30, 0.35, 0.40,0.45, 0.50, 0.60, 0.70, 0.80 mA. These were single pulses with an interstimulus interval of 60 seconds.  Electrode stability was also monitored throughout the course of the experiment by plotting electrode thickness and electrode calibration factors across the placement order to determine if experimental aspects were significantly affecting sensitivity or fouling throughout the course of the experiment. \ No animals were excluded, however signals from individual brain regions were excluded according to the following exclusion criteria: if the Signal: Noise ratio was less than 3:1, if the placement was later determined to be outside of the region of interest, if a stimulation artifact obscured the true dopamine peak, or if the dopamine release or uptake was statistically determined to be an outlier. In all data files, a cell containing the value ‘NA’ indicates that that data point was excluded for one of the reasons outlined above. In all data files, cells containing the value ‘null’ are intentionally left blank, and do not represent excluded data.  Some stability slices were excluded due to air bubbles forming under the slice, which caused the electrode to be pushed through the tissue. The data file ‘Stability Data.xlsx’ contains justifications for the exclusion of these slices in the final row of each slice’s data, on the excel sheets ‘mouse stability slices’ and ‘rat stability slices’, for mice and rats respectively. This data file also contains the parameters used to determine stability in all slices on the sheets ‘by subregion’ and ‘by placement order’, which include identifying information regarding stability slice (‘slice/folder’), the placement order (‘placement’), calibration factor of the electrode (‘Cal factor’) and the electrode thickness as calculated via the Michaelas-Menten equation (‘Thickness’). Figures displaying slice viability across time, calibration factor across placement order and subregion, and electrode thickness across placement order and subregion are presented in the file ‘Slice and Electrode Stability Controls.pdf’.  Electrode placements are recorded in the data file ‘Placements.pdf’. All placements were marked on images from the atlas (Paxinos, 2013), including placements that were later excluded from the data set for a reason outlined above. Only placements that did not result in a stable baseline were excluded from this data file.  The data file ‘Volt Baseline Data.xlsx’ contains the identifying information ‘animal’, ‘slice’, ‘sex’, ‘subregion’, ‘z-plane’, and ‘placement order’, as well as the stable dopamine release (‘baseline µM’ & ‘baseline DA/p’) and uptake (‘Baseline Vmax’) data for all slices. Data on all excel sheets are from both males and females, as indicated in the column ‘sex’, with male and female data separated by a row of dark grey cells. Data on the excel sheets ‘mice ratios’ and ‘rat ratios’ are separated by subregion and contain the stable dopamine release (‘baseline µM’ & ‘baseline DA/p’) and uptake (‘Baseline Vmax (nM/s)’) data for all slices, in addition to the ratio of ‘baseline µM’ divided by ‘Vmax (µM/s)’ for each slice. These data can be viewed as figures in the manuscript, along with the corresponding statistical analyses.   The data files ‘Mouse Data.xlsx’ and ‘Rat Data.xlsx’ contain the results of the stimulation variation experiments. Individual slices are identified by the identifying information ‘animal’, ‘slice’, ‘sex’, ‘subregion’, ‘z-plane’, and ‘placement order’. For female animals only, the phase of the estrus cycle identified at time of tissue dissection is recorded in the column ‘cycle stage’. The column ‘stimulation’ designates the applied stimulation amplitude (mA) and the column ‘output µM’ indicates the dopamine release at that stimulation intensity (µM). Evoked dopamine concentration data from the baseline file for each subregion placement are in the columns ‘baseline µM’ and ‘baseline DA/p’, and dopamine uptake values are in the column ‘Baseline Vmax’.   ##### Change log May 28, 2024 The changes made are as follows: In the files Mouse_Data and Rat_Data the stimulation value in column J was shifted by a factor of 10 to account for a previous error. These values were also corrected in the ReadMe.