DOI: 10.5061/dryad.hmgqnk9t1
title: Measurement of biochemical Marker Dynamics in serum levels of calcium, phosphorus, P1NP, and CTX across three time points (T0, T1, T2) following treatment with dimeric R25CPTH(1-34), rhPTH(1-34), and control

description: Introduction: Despite continuous parathyroid hormone (PTH) exposure
 potentially accelerating bone resorption, intermittent PTH administration
 has shown anabolic effects on bone microarchitecture. This study
 investigates the therapeutic impacts of two PTH analogs, rhPTH(1-34) and
 dimeric R25CPTH(1-34) on bone regeneration and osseointegration in a
 postmenopausal osteoporosis animal model. Methods: Twelve female beagles,
 osteoporotic post-ovariectomy, underwent implant surgeries. Animals were
 divided into three groups: control, daily rhPTH(1-34) injection, and daily
 dimeric R25CPTH(1-34) injection. After 10 weeks, bone regeneration and
 implant osseointegration were evaluated using micro-CT,
 histological/histomorphometric analyses, and serum biochemical analysis.
 Results: While the rhPTH(1-34) group demonstrated improved
 microarchitectural characteristics, such as BMD, BV, trabecular metrics,
 and osseointegration degree, the dimeric R25CPTH(1-34) group showed
 similarly enhanced anabolic effects around the titanium implants, albeit
 these were less pronounced than the rhPTH(1-34), yet significantly better
 than the control group. Histologic and TRAP assays revealed that both PTH
 analogs significantly promoted bone regeneration and remodeling,
 especially in artificially created bone defects. Conclusion: This study
 demonstrated the therapeutic effects of rhPTH(1-34) and dimeric
 R25CPTH(1-34) on bone regeneration and titanium osseointegration in a
 beagle model with osteoporosis.
description: Fasting blood samples were drawn in the morning at baseline (T0;
 12 weeks), the start of the injection at 26th weeks(T1), and euthanasia at
 36th weeks (T2) for P1NP (procollagen type I N-terminal propeptide), PTH
 (parathyroid hormone), CTx (C-terminal telopeptide), and calcium, and
 phosphorus (Figure 5A). Calcium and phosphorus were analyzed using the
 Beckman AU480 Chemistry Analyzer (Beckman Coulter AU480). P1NP
 (procollagen type I N-terminal propeptide ELISA kit, Mybiosource), PTH
 (parathyroid hormone ELISA kit, Aviva Systems Biology), and CTX
 (C-terminal telopeptide (CTx-I) ELISA kit, Mybiosource) were analyzed by
 the ELISA method, according to the ELISA kit manufacturer’s instructions.
 In all analyses, the measured values were below the limit of
 quantification for the standard curve.
description: # Measurement of biochemical Marker Dynamics in serum. The serum levels of
 calcium, phosphorus, P1NP, and CTX across three time points (T0, T1, T2)
 following treatment with dimeric R25CPTH(1-34), rhPTH(1-34), and control
 [https://doi.org/10.5061/dryad.hmgqnk9t1](https://doi.org/10.5061/dryad.hmgqnk9t1) ## Description of the data and file structure The serum levels of calcium, phosphorus, P1NP, and CTX across three time points (T0, T1, T2) following treatment with dimeric R25CPTH(1-34), rhPTH(1-34) and control. Calcium and phosphorus levels show an upward trend in response to both PTH treatments compared to control, indicating enhanced bone mineralization. P1NP levels, indicative of bone formation, remain relatively stable across time and treatments. CTX levels, associated with bone resorption, show no significant differences between groups. Data points for the dimeric R25CPTH(1-34), rhPTH(1-34), and control are marked by squares, circles, and triangles, respectively, with error bars representing confidence intervals. Fasting blood samples were drawn in the morning at baseline (T0; 12 weeks), the start of the injection at 26th weeks(T1), and euthanasia at 36th weeks (T2) for P1NP (procollagen type I N-terminal propeptide), PTH (parathyroid hormone), CTx (C-terminal telopeptide), and calcium, and phosphorus (Figure 5A). Calcium and phosphorus were analyzed using the Beckman AU480 Chemistry Analyzer (Beckman Coulter AU480). P1NP (procollagen type I N-terminal propeptide ELISA kit, Mybiosource), PTH (parathyroid hormone ELISA kit, Aviva Systems Biology), and CTX (C-terminal telopeptide (CTx-I) ELISA kit, Mybiosource) were analyzed by the ELISA method, according to the ELISA kit manufacturer’s instructions. In all analyses, the measured values were below the limit of quantification for the standard curve. ## Sharing/Access information Data was derived from GraphPad Prism