DOI: 10.5061/dryad.nk98sf81g
title: Data from: Pollinator and flower morphology interact to influence pollen receipt

description: Pollinators are important drivers of floral divergence and speciation in
 plants. Here we investigate the effects of floral trait variation and
 trait matching on pollen receipt in two different plant species, each
 visited by different functional pollinator types across their ranges.
 Using single visits by pollinators to flowers in populations with
 experimentally increased variance in floral morphology, we demonstrate
 that distinct functional groups of pollinators affect pollen movement
 differently through their interactions with floral trait variation. This
 study provides details of how the mechanical fit between floral and
 pollinator morphology may drive the evolution of floral traits through its
 effects on pollen deposition both within and between populations with
 contrasting pollinators.
description: We used single-visit experiments to understand the relationship
 between a component of female fitness (pollen receipt) and flower
 morphology, in association with insects that vary in their morphological
 traits that affect pollination.  Insect
 morphology was collected by capture with a butterfly net and measurements
 were taken with a steel ruler or callipers Floral
 morphology was collected by cutting bolting inflorescences at the base
 from the field, and floral traits were measured to the nearest millimetre
 using a steel ruler or digital set of callipers
 Inflorescences containing flowers with receptive stigmas were
 used to conduct single visits where virgin flowers were exposed to
 pollinators and pollinators were allowed to visit a flower only once.
 After this event, the stigmas were removed and embedded in fuchsin gel
 from which the pollen grains were counted. With Nerine humilis that have
 large pollen grains, pollen was counted directly on the stigma after a
 single visit in the same afternoon under a disection
 microscope.
description: # Data from: Pollinator and flower morphology interact to influence pollen
 receipt This file READ_ME_Performance_Surfaces.txt was generated on
 30/03/2024 ## GENERAL INFORMATION: 1. Title of Dataset: Pollinator and
 flower morphology interact to influence pollen receipt 2. Author
 Information: 3. Date of data collection: March and April 2015 to 2024 4.
 Field locations of data collection: Main experimental sites: A. Barrydale,
 Western Cape, South Africa (-33.935997°S, 20.679453°E) B. Swartberg,
 Western Cape, South Africa (-33.362594°S, 22.068132°E) C. Nuwekloof Pass,
 Baviaanskloof, Eastern Cape, South africa (-33.511291°S, 23.642786°E) D.
 Swellendam, Western Cape, South Africa (-34.184574°S, 20.291919°E) 5.
 Funding sources: ## DATA AND FILE OVERVIEW ### 1. Description of dataset
 Our dataset investigates the interaction between flower and pollinator
 morphology in influencing pollen receipt surfaces in two geophytes, Nerine
 humilis and Tritoniopsis revoluta. Data in these files have primarily been
 collected from our four field sites that represented a main
 'native' site of each of the taxa, we also introduced variation
 from close populations in order to increased variance in each population.
 This allowed us to more easily determine the form and strength of
 selection. ### 2. File list File 1 Name:1. Proboscis_Measurements.csv
 Description: Proboscis measurements of all insects from our experimental
 populations. File 2 Name:2. Plant_Trait_Variation.csv Description: Floral
 trait measurements associated with the standing variation and increased
 variation for each species, from each population. File 3 Name:3.
 Swellendam_Full_Datasetcsv Description: Full dataset associated with
 single visits for the short style locality, Swellendam. This dataset was
 specifically used to generate performance surfaces and selection
 coefficients for the short style Nerine humilis. File 4 Name:4.
 Baviaanskloof_Full_Dataset.csv Description: Full dataset associated with
 single visits for the long style locality, Baviaanskloof. This dataset was
 specifically used to generate performance surfaces and selection
 coefficients for the long style Nerine humilis. File 5 Name:5.
 Barrydale_Full_Dataset.csv Description: Full dataset associated with
 single visits for the long tube locality, Barrydale. This dataset was
 specifically used to generate performance surfaces and selection
 coefficients for the long tubed Tritoniopsis revoluta. File 6 Name:6.
 Swartberg_Full_Dataset.csv Description: Full dataset associated with
 single visits for the short tubed locality, Swartberg. This dataset was
 specifically used to generate performance surfaces and selection
 coefficients for the short tubed Tritoniopsis revoluta. File 7 Name:6.
 Swartberg_Full_DatasetR60.csv Description: Full dataset associated with
 single visits for the short tubed locality, Swartberg. This dataset was
 specifically used to generate selection coefficients for short proboscid
 insects associated with the short tubed Tritoniopsis revoluta. We removed
 one outlier from this particular dataset which gave a significant linear
 coefficient when present (see Figure 2F), as in the
 "Swartberg_Full_Dataset.csv". This dataset is the same as file
 6, but without row 60. ### 3. Methodological information (A) Morphological
 data: Files 1 and 2 containing raw data associated with calculating
 descriptive statistics, specifically means and standard deviations. File 1
 is associated with proboscis measurements and File 2 contains data
 associated with local and increased variation at our experimental
 localities. (B) Performance surfaces and coefficients Files 3-7 contains
 data associated with single visits based on native and increased variance.
 This raw data was used to generate pollen receipt surfaces and calculate
 selection coefficients. ## DATA SPECIFIC INFORMATION FOR EACH FILE ###
 FILE: 1. Proboscis_Measurements.csv (A) Number of variables: 6 (B) Number
 of rows: 62 (including headers) (C) Variable list: * Date: Date insect was
 caught. * Pollinator: Pollinator species * Locality: Locality where caught
 * Caught visiting: Flower pollinator was caught on * Extended proboscis
 length (mm): The extended proboscis length measurement of each insect *
 Functional Body Length (mm): Body length measurement associated with
 affecting pollination (D) Missing data codes: 37 cells under
 "Functional Body Length (mm)" starting from lines 29 to 65 (E)
 Abbreviations used: None (F) Other relevant information: None ### FILE: 2.
 Plant_Trait_Variation.csv (A) Number of variables: 4 (B) Number of rows:
 441 (including headers) (C) Variable list: * Species: Taxon * Locality:
 Locality of taxon sampled * Natural Variation: Natural variation
 associated with the trait collected for each taxon * Extended Variation:
 Increased variation associated with the trait collected for each taxon.
 (D) Missing data codes: None (E) Abbreviations used: None (F) Other
 relevant information: Data is only missing because of unequal sampling
 between different taxa for natural versus extended variation columns ###
 FILE: 3. Swellendam_Full_Dataset.csv (A) Number of variables: 4 (B) Number
 of rows: 146 (including headers) (C) Variable list: * Source: Source of
 trait variation used in single visit experiment * Pollinator: Pollinator
 taxon visiting flower * Style length (mm): Style length of flower used in
 single visit experiment * Pollen: Pollen grains deposited per single visit
 (D) Missing data codes: None (E) Abbreviations used: None (F) Other
 relevant information: None ### FILE: 4. Baviaanskloof_Full_Dataset.csv (A)
 Number of variables: 4 (B) Number of rows: 70 (including headers) (C)
 Variable list: * Source: Source of trait variation used in single visit
 experiment * Pollinator_species: Pollinator taxon visiting flower * Style
 length (mm): Style length of flower used in single visit experiment *
 Pollen: Pollen grains deposited per single visit (D) Missing data codes:
 None (E) Abbreviations used: None (F) Other relevant information: None ###
 FILE: 5. Barrydale_Full_Dataset.csv (A) Number of columns : 5 (B) Number
 of rows: 55 (including headers) (C) Variable list: *
 Functional_pollinator_type:Describes the functional group of pollinators
 associated with a single visit * pollinator: Pollinator species *
 tube_length_(mm): Tube length of flower used in single visit * pollen:
 number of pollen grains recorded associated with single visit event *
 visitor: code for functional pollinator group used in "by"
 command for generalized additive models (D) Missing data codes: None (E)
 Abbreviations used: \[SPI] - Short probscid insect \[LTF] - Long proboscid
 fly (F) Other relevant information: visitor code is directly linked to
 functional pollinator groups defined in (E) directly above ### FILE: 6.
 Swartberg_Full_Dataset.csv (A) Number of columns : 5 (B) Number of rows:
 100 (including headers) (C) Variable list: *
 Functional_pollinator_type:Describes the functional group of pollinators
 associated with a single visit * Pollinator: Pollinator species *
 tube_length_(mm): Tube length of flower used in single visit * pollen:
 number of pollen grains recorded associated with single visit event *
 visitor: code for functional pollinator group used in "by"
 command for generalized additive models (D) Missing data codes: None (E)
 Abbreviations used: \[LTF] - Long proboscid fly \[SPI] - Short probscid
 insect (F) Other relevant information: visitor code is directly linked to
 functional pollinator groups defined in (E) directly above ### FILE: 7.
 Swartberg_Full_DatasetR60.csv (A) Number of columns: 5 (B) Number of rows:
 99 (including headers) (C) Variable list: * Functional_pollinator_type:
 Describes the functional group of pollinators associated with a single
 visit * Pollinator: Pollinator species * tube_length_(mm): Tube length of
 flower used in single visit * pollen: number of pollen grains recorded
 associated with single visit event * visitor: code for functional
 pollinator group used in "by" command for generalized additive
 models (D) Missing data codes: None (E) Abbreviations used: \[LTF] - Long
 proboscid fly \[SPI] - Short probscid insect (F) Other relevant
 information: visitor code is directly linked to functional pollinator
 groups defined in (E) directly above. Important! Row 60 was removed in
 this dataset associated with the above dataset
 "Swartberg_Full_Dataset.csv" because of an outlier that gave a
 significant postive linear relationship.