DOI: 10.6075/j0k07458
title: CIL:33225, Homo sapiens, endocrine-paracrine cell of prostate gland, basal cell of prostate epithelium, luminal cell of prostate epithelium, blood vessel endothelial cell, perineural cell, sheath cell, leukocyte, prostate stromal cell, smooth muscle cell of prostate. In Cell Image Library

description: Tissue section of human prostate containing adenocarcinoma that has been immunostained for the cell-surface antigen CD44. Nuclei are stained in blue.This image is part of a large collection of images generated from numerous specimens to characterize the distribution of CD44 in human prostate tissue. A summary of the entire data set is provided below.
 
Cell Type, % intense, % equivocal, % none, # assays,
Atrophic glands:, 5, 20, 75, 2, 
Hyperplastic glands:, 10, 0, 90, 1, 
Normal glands:, 0, 0, 100, 2, 
Basal Epithelial Cells:, 25, 45, 30, 2, 
Stromal Endothelial Cells:, 0, 0, 100, 2, 
Stromal Fibromuscular Cells:, 0, 0, 100, 2, 
Stromal Nerve Sheath Cells:, 10, 10, 80, 1, 
Stromal Perineural Cells:, 0, 0, 100, 1, 
Gleason Pattern 3:, 18, 15, 68, 2, 
Gleason Pattern 4:, 18, 15, 68, 2, 
Gleason Pattern 5:, 15, 0, 85, 1
 
The table indicates the percent of each cell type with the indicated staining intensity. Staining intensity levels were classified as intense, equivocal (weak or very focal) or none. Not all of the cell types and staining patterns are visible in each image.
 
This image is part of a large collection of immunohistochemistry images of cell-surface antigens generated by the SCGAP Urologic Epithelial Stem Cells (UESC) Project. The overall goal of the project is to characterize and isolate epithelial stem cell populations from two urologic organs, the prostate and bladder. Links are provided below for the UESC Project database, the entire human prostate immunostain summary, the CD44 immunostain summary, and information on the specimen that this image is from. Other images of CD44 human prostate immunostains are accessible following the group link.
description: The "Image ID" for this image is provided in the Attribution section. Because the images are systematically named, information such as the antibody used in staining, the organism, tissue type, tissue block, and magnification are all encoded in the Image ID. As an example, the image 'CD44 98-395F HP ba 100.jpg' was stained with anti-CD44, was derived from human prostate tissue block 98-395F, is human prostate tissue (HP), and an image of microscopic field of view b within field a was captured at 100× magnification.


Complete technical details available in the Protocol Section of SCGAP UESC website (link provided). Briefly, radical prostatectomy specimens were transverse-sectioned 3mm thick and tissue blocks were embedded in precooled OCT (Tissue-tek) medium. Frozen tissue blocks were thin-sectioned (5µ) and fixed in cold acetone. A monoclonal primary antibody (PharMingen) was used followed by a biotinylated secondary antibody. Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). Sections were lightly counterstained with hematoxylin. Images were acquired using a digital camera and either an Olympus BX41 or Olympus BH2 microscope.
description: Preparation: acetone fixed tissue; tissue in polyethylene glycol embedment
Relation to intact cell: cryostat-sectioned tissue
Item type: recorded image
Imaging mode: widefield illumination; bright-field microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein; differences in intrinsic optical density
Visualization methods: hematoxylin; primary antibody plus labeled secondary antibody; horseradish peroxidase; 3,3'-diaminobenzidine (DAB); biotin-avidin amplification
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation