cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.57760/sciencedb.15277
title: Whole genome re-sequencing and breseq analysis of ΔPvs1 and ΔpMBL43001
title: Whole genome re-sequencing and breseq analysis of ΔPvs1 and ΔpMBL43001

description: In our study, we developed the Cas9-NE method, which combines the CRISPR/Cas9 system with the natural excision process of mobile genetic elements (MGEs). By utilizing the Cas9-NE method, both the integrative and circular forms of MGEs can be accurately and completely eliminated through Cas9 cleavage, resulting in the generation of MGE-removed cells. We successfully applied the Cas9-NE method to remove four representative MGEs, namely plasmid pMBL43001, prophages GfP2 and Pvs1, and genomic islands VPII, from Vibrio strains.To investigate if any additional mutations occurred after the Cas9/sgRNA treatment, we conducted whole genome re-sequencing and performed breseq (version 0.37.1) analysis. Specifically, we compared an independent mutant ΔPvs1 with its corresponding wild-type strain Vibrio shilonii SCSIO 43133, as well as an independent mutant ΔpMBL43001 with its corresponding wild-type strain Vibrio coralliilyticus SCSIO 43001. The results of the analysis revealed no additional mutations in the sequenced ΔPvs1 and ΔpBML43001 mutants.This finding suggests that the Cas9-NE method specifically targets and removes the desired MGEs without inducing unintended mutations in the genome. It highlights the precision and accuracy of the Cas9-NE method in eliminating MGEs while maintaining the genomic integrity of the targeted strains.
description: In our study, we developed the Cas9-NE method, which combines the CRISPR/Cas9 system with the natural excision process of mobile genetic elements (MGEs). By utilizing the Cas9-NE method, both the integrative and circular forms of MGEs can be accurately and completely eliminated through Cas9 cleavage, resulting in the generation of MGE-removed cells. We successfully applied the Cas9-NE method to remove four representative MGEs, namely plasmid pMBL43001, prophages GfP2 and Pvs1, and genomic islands VPII, from Vibrio strains.To investigate if any additional mutations occurred after the Cas9/sgRNA treatment, we conducted whole genome re-sequencing and performed breseq (version 0.37.1) analysis. Specifically, we compared an independent mutant ΔPvs1 with its corresponding wild-type strain Vibrio shilonii SCSIO 43133, as well as an independent mutant ΔpMBL43001 with its corresponding wild-type strain Vibrio coralliilyticus SCSIO 43001. The results of the analysis revealed no additional mutations in the sequenced ΔPvs1 and ΔpBML43001 mutants.This finding suggests that the Cas9-NE method specifically targets and removes the desired MGEs without inducing unintended mutations in the genome. It highlights the precision and accuracy of the Cas9-NE method in eliminating MGEs while maintaining the genomic integrity of the targeted strains.
DOI: 10.57760/sciencedb.15285
title: Study on compressive-shear strength test and constitutive injured model of frozen simulated lunar soil with low temperature
title: Study on compressive-shear strength test and constitutive injured model of frozen simulated lunar soil with low temperature

description: The uniaxial compressive strength test and direct shear strength test of frozen simulated lunar soil were carried out under different temperatures (-70°C, -50°C, -30°C, -10°C) and different water contents ( 5wt%, 7wt%, 9wt%, 11wt%).The unit of compressive strength is MPa, and the unit of shear strength is KPa.
description: The uniaxial compressive strength test and direct shear strength test of frozen simulated lunar soil were carried out under different temperatures (-70°C, -50°C, -30°C, -10°C) and different water contents ( 5wt%, 7wt%, 9wt%, 11wt%).The unit of compressive strength is MPa, and the unit of shear strength is KPa.
DOI: 10.57760/sciencedb.15293
title: Candida count
title: Candida count

description: The colony of C albicans after treated with photosensitizers and Green Light-emitting diode.
description: The colony of C albicans after treated with photosensitizers and Green Light-emitting diode.
DOI: 10.57760/sciencedb.15295
title: Uncover the survival mechanism of bacteria and phages in glacial forelands
title: Uncover the survival mechanism of bacteria and phages in glacial forelands

description:  Here, we captured a paired analysis of metagenomes and viromes sampled from a 130 year of Gongga mountain glacial retreat chronosequence. 
description:  Here, we captured a paired analysis of metagenomes and viromes sampled from a 130 year of Gongga mountain glacial retreat chronosequence.
DOI: 10.57760/sciencedb.j00139.00106
title: Study of spermine on prednisolone-treated zebrafish larvae
title: Study of spermine on prednisolone-treated zebrafish larvae

description: RNA sequencing (RNA-Seq) was conducted in 7 dpf zebrafish larvae received prednisolone (PN) only (OP group) and PN with spermine (SPM) (SPM group), aiming at revealing the key molecular mediating the anti-osteoporosis effect of spermine.
description: RNA sequencing (RNA-Seq) was conducted in 7 dpf zebrafish larvae received prednisolone (PN) only (OP group) and PN with spermine (SPM) (SPM group), aiming at revealing the key molecular mediating the anti-osteoporosis effect of spermine.
DOI: 10.57760/sciencedb.15300
title: vortex beam
title: 涡旋光束

description: high oder harmonic generation with orbital angular momentum
description: high oder harmonic generation with orbital angular momentum
DOI: 10.57760/sciencedb.15302
title: water flow data
title: water flow data

description: 河北平原城市水资源数据.
description: 河北平原城市水资源数据.
DOI: 10.57760/sciencedb.space.01613
title: Study on anomalies of integrated operational amplifier induced by proton deep charge-discharge effects
title: 质子深层充放电效应诱发运算放大电路异常的研究

description: With the gradual development of the aerospace industry towards deep space exploration, the frequency of space activities in harsh space environments such as the Van Allen Belt, the surface of Mars, or other planets in the solar system is gradually increasing The radiation environment encountered by spacecraft in interplanetary deep space orbits is mainly composed of solar protons and galactic cosmic rays. When a large number of high-energy protons gather in the spacecraft's dielectric materials, it will induce charging and discharging effects, thereby affecting electronic devices This article takes the LM124 operational amplifier circuit as a research example, and uses a medium to high energy proton accelerator to study the characteristic laws of anomalies induced by deep space proton induced deep charge discharge effects in the circuit. At the same time, combined with HSPICE simulation methods, the sensitive areas and mechanisms of the discharge pulse action operational amplifier circuit are studied 
description: With the gradual development of the aerospace industry towards deep space exploration, the frequency of space activities in harsh space environments such as the Van Allen Belt, the surface of Mars, or other planets in the solar system is gradually increasing The radiation environment encountered by spacecraft in interplanetary deep space orbits is mainly composed of solar protons and galactic cosmic rays. When a large number of high-energy protons gather in the spacecraft's dielectric materials, it will induce charging and discharging effects, thereby affecting electronic devices This article takes the LM124 operational amplifier circuit as a research example, and uses a medium to high energy proton accelerator to study the characteristic laws of anomalies induced by deep space proton induced deep charge discharge effects in the circuit. At the same time, combined with HSPICE simulation methods, the sensitive areas and mechanisms of the discharge pulse action operational amplifier circuit are studied
DOI: 10.57760/sciencedb.j00186.00400
title: Dataset for Compton scattering events discrimination in high-purity germanium γ-ray spectrometer
title: Dataset for Compton scattering events discrimination in high-purity germanium γ-ray spectrometer

description: The uploaded content mainly consists of three parts: original images, tables, and the Origin project file that contains data and plots. The data in the Origin project file forms the raw data for the figures in the article. With the data in the Origin file, figures can be generated, and detailed analysis of the figures’ details can be performed by analyzing the differences in the data in the Origin file.
description: The uploaded content mainly consists of three parts: original images, tables, and the Origin project file that contains data and plots. The data in the Origin project file forms the raw data for the figures in the article. With the data in the Origin file, figures can be generated, and detailed analysis of the figures’ details can be performed by analyzing the differences in the data in the Origin file.
DOI: 10.57760/sciencedb.15311
title: Transcriptome analysis (RNA-seq) data of Coilia naus with different rates of ovarian development
title: 卵巢发育速度不同的刀鲚的转录组分析（RNA-seq）数据

description: Coilia nasus, a migratory fish species naturally inhabiting in the middle and lower reaches of the Yangtze River and offshore China, has high culturing potential and economic value. In this study, we performed transcriptome analysis (RNA-seq) of C. naus with different rates of ovarian development to explore candidate genes and signal pathways involved in the population-asynchronous ovarian development of C. nasus.
description: Coilia nasus, a migratory fish species naturally inhabiting in the middle and lower reaches of the Yangtze River and offshore China, has high culturing potential and economic value. In this study, we performed transcriptome analysis (RNA-seq) of C. naus with different rates of ovarian development to explore candidate genes and signal pathways involved in the population-asynchronous ovarian development of C. nasus.
DOI: 10.57760/sciencedb.15313
title: Serum Metabolome of Lactating Dairy Cows
title: Serum Metabolome of Lactating Dairy Cows

description: These datas are about serum metabolome of lactating dairy cows.
description: These datas are about serum metabolome of lactating dairy cows.
DOI: 10.57760/sciencedb.j00113.00200
title: The data of the article "Error field penetration in J-TEXT tokamak based on two-fluid drift-MHD model"
title: The data of the article "Error field penetration in J-TEXT tokamak based on two-fluid drift-MHD model"

description: The date related to the article "Error field penetration in J-TEXT tokamak based on two-fluid drift-MHD model" was accepted in the journal of Chinese Physics B. It contains a text file, which is described below:In this paper, we have studied the plasma responses using Fitzpatrick’s improved two-fluid model and program LAYER. We calculated the error field penetration threshold for J-TEXT.
description: The date related to the article "Error field penetration in J-TEXT tokamak based on two-fluid drift-MHD model" was accepted in the journal of Chinese Physics B. It contains a text file, which is described below:In this paper, we have studied the plasma responses using Fitzpatrick’s improved two-fluid model and program LAYER. We calculated the error field penetration threshold for J-TEXT.
DOI: 10.57760/sciencedb.15544
title: Transcriptome sequencing in hibernating and aestivating species
title: Transcriptome sequencing in hibernating and aestivating species

description: Transcriptome sequencing in hibernating and aestivating species
description: Transcriptome sequencing in hibernating and aestivating species
DOI: 10.57760/sciencedb.15325
title: The Influence of Just World Beliefs on Internet Altruistic Behavior among College Students: the Roles of Gratitude and Online Social Support
title: The Influence of Just World Beliefs on Internet Altruistic Behavior among College Students: the Roles of Gratitude and Online Social Support

description: Just world beliefs may influence college students' Internet altruistic behaviors directly or indirectly through a mediating effect. This study investigated the relationships between belief in a just world (BJW), gratitude, online social support, and Internet altruistic behavior (IAB) in order to enhance our comprehension of the impact of BJW on IAB. Methods: We conducted a survey using questionnaires to investigate the relationship and underlying mechanisms between BJW, gratitude, online social support, and IAB among 802 college students. Results: (a) BJW could significantly predict IAB; (b) The relationship between BJW and IAB is partially mediated by gratitude; (c) Online social support plays a moderating role in the relationship between BJW and IAB. Specifically, for individuals with high online social support, BJW directly predicts IAB. However, for individuals with low online social support, the predictive effect of BJW on IAB is not significant. Conclusions: These findings indicate that gratitude and online social support play different roles in the relationships between BJW and IAB, and suggest these relationships have unique mechanisms. These results also highlight the importance of gratitude and online social support in comprehending IAB.
description: Just world beliefs may influence college students' Internet altruistic behaviors directly or indirectly through a mediating effect. This study investigated the relationships between belief in a just world (BJW), gratitude, online social support, and Internet altruistic behavior (IAB) in order to enhance our comprehension of the impact of BJW on IAB. Methods: We conducted a survey using questionnaires to investigate the relationship and underlying mechanisms between BJW, gratitude, online social support, and IAB among 802 college students. Results: (a) BJW could significantly predict IAB; (b) The relationship between BJW and IAB is partially mediated by gratitude; (c) Online social support plays a moderating role in the relationship between BJW and IAB. Specifically, for individuals with high online social support, BJW directly predicts IAB. However, for individuals with low online social support, the predictive effect of BJW on IAB is not significant. Conclusions: These findings indicate that gratitude and online social support play different roles in the relationships between BJW and IAB, and suggest these relationships have unique mechanisms. These results also highlight the importance of gratitude and online social support in comprehending IAB.
DOI: 10.57760/sciencedb.15327
title: Tex29, a novel acrosome marker but dispensable for spermatogenesis and fertilization
title: Tex29, a novel acrosome marker but dispensable for spermatogenesis and fertilization

description: a novel acrosome marker Tex29this database contain experiment results of Tex29
description: a novel acrosome marker Tex29this database contain experiment results of Tex29
DOI: 10.57760/sciencedb.15332
title: Forecasting Interval Valued Stock Returns Based on News Media Sentiments_data and code
title: 基于新闻文本情绪的区间值股票回报预测研究_数据与代码

description: Data and code are used to analyze the data and results in the article “Forecasting Interval Valued Stock Returns Based on News Media Sentiments.”1. The sample period of the basic data is the weekly data from 2014/01/30 to 2022/10/14.2, data.csv and data_index.xlsx are the basic data sets, for the subsequent results of the foundation of the analysis; the notation sz, yysw, fyjr, yh, dz, jsj, nlmy in table represent the SZSE index, pharmaceuticals and biologicals, non-banking financial, banking, electronics, computers, and agriculture, forestry, animal husbandry and fisheries industry, respectively, corresponding to the content of article. The rest of the data files are derived from the analysis process and used for analysis, corresponding to the graphics and tables in our article, see the comments in our code file for details.3、In our table, the notation "max" and "min" represent the sequence of maximum and minimum values; "p" represents the sequence of price index, "D" represents the sequence of difference, and "ln" represents the sequence of logarithm.4. The code is in R language, corresponding to the results of the charts in the text, see the comments in the code.
description: Data and code are used to analyze the data and results in the article “Forecasting Interval Valued Stock Returns Based on News Media Sentiments.”1. The sample period of the basic data is the weekly data from 2014/01/30 to 2022/10/14.2, data.csv and data_index.xlsx are the basic data sets, for the subsequent results of the foundation of the analysis; the notation sz, yysw, fyjr, yh, dz, jsj, nlmy in table represent the SZSE index, pharmaceuticals and biologicals, non-banking financial, banking, electronics, computers, and agriculture, forestry, animal husbandry and fisheries industry, respectively, corresponding to the content of article. The rest of the data files are derived from the analysis process and used for analysis, corresponding to the graphics and tables in our article, see the comments in our code file for details.3、In our table, the notation "max" and "min" represent the sequence of maximum and minimum values; "p" represents the sequence of price index, "D" represents the sequence of difference, and "ln" represents the sequence of logarithm.4. The code is in R language, corresponding to the results of the charts in the text, see the comments in the code.
DOI: 10.57760/sciencedb.15335
title: Sub-GeV events energy reconstruction with 3-inch PMTs in JUNO
title: Sub-GeV events energy reconstruction with 3-inch PMTs in JUNO

description: Electrons which are the Monte Carlo data samples in this energy reconstruction at different energies. We generated the samples using JUNO software framework.
description: Electrons which are the Monte Carlo data samples in this energy reconstruction at different energies. We generated the samples using JUNO software framework.
DOI: 10.57760/sciencedb.15342
title: data for Intrinsic Switchable Valley Polarized Photocurrent in ε-InSe
title: data for Intrinsic Switchable Valley Polarized Photocurrent in ε-InSe

description: Valleytronics, focusing on the charge carriers occupies in valleys (local energy extrema) of the energy bands in semiconductors, demonstrate an additional degree of freedom besides the spin and charge. Usually, the inequivalent valley ‘states’ in transition metal disulfides (TMDs) could be manipulated by external magnetic fields by controlling the valley-polarized carriers in spin-coupled valleys. Here, we found the valley polarization of the photocurrent in ε-InSe could be intrinsically manipulated without the external field by the all-optical contactless method. The transient circular photogalvanic effect (CPGE) induced photocurrent revealed by terahertz emission could be reversed only by selected pumping carriers from vertically split valence bands into different Rashba valleys. The switchable Rashba valley polarization, i. e. the optical orienting valley degree of freedom, could be fulfilled by the synergetic effects between momentum-dependent Zeeman-like field due to the emergent nonzero Berry curvature and the Rashba splitting. Our results suggest the spontaneous symmetry broken could degenerate the given valley in ε-InSe and give a new roadmap for the manipulation of the valley degree of freedom intrinsically. 
description: Valleytronics, focusing on the charge carriers occupies in valleys (local energy extrema) of the energy bands in semiconductors, demonstrate an additional degree of freedom besides the spin and charge. Usually, the inequivalent valley ‘states’ in transition metal disulfides (TMDs) could be manipulated by external magnetic fields by controlling the valley-polarized carriers in spin-coupled valleys. Here, we found the valley polarization of the photocurrent in ε-InSe could be intrinsically manipulated without the external field by the all-optical contactless method. The transient circular photogalvanic effect (CPGE) induced photocurrent revealed by terahertz emission could be reversed only by selected pumping carriers from vertically split valence bands into different Rashba valleys. The switchable Rashba valley polarization, i. e. the optical orienting valley degree of freedom, could be fulfilled by the synergetic effects between momentum-dependent Zeeman-like field due to the emergent nonzero Berry curvature and the Rashba splitting. Our results suggest the spontaneous symmetry broken could degenerate the given valley in ε-InSe and give a new roadmap for the manipulation of the valley degree of freedom intrinsically.
DOI: 10.57760/sciencedb.j00217.00324
title: Using HTS-ELISA method to make anti SP-10 couple monoclonal antibody
title: 应用HTS-ELISA筛选方法制备抗精子顶体蛋白SP-10的配对单克隆抗体

description: Objective To prepare specificity recognize different epitopes of SP-10 couple monoclonal antibodies.Methods Balb/C mice were immunized with SP-10, and screened secret anti-SP-10 monoclonal antibody hybridoma by High Throughput Screening ELISA（HTS-ELISA）and prepared monoclonal antibodies. Then HRP labeling was used to analyze the characteristics of monoclonal antibodies recognizing different epitopes of SP-10. Results Three hybridoma cell lines were screened with monoclonal antibodies secreting specific recognition of SP-10 protein. The monoclonal antibodies secreted by them were named mAb80, mAb81 and mAb208, in which the titer of mAb80 and mAb81 was more than 1:1×107 and the titer of mAb208 was 1:2×106, and the epitopes of mAb208 recognizing SP-10 were significantly different from those of mAb80 and mAb81, showing that they were couple monoclonal antibodies. A sandwich ELISA for the detection of SP-10 was constructed by using mAb208 labeled HRP as the detection antibody and mAb81 as the capture antibody. The minimum detection limit of SP-10 was up to 0.01 ng/mL, the linear range was 0.01-10 ng/mL, and the ELISA recovery of SP-10 with 0.05, 0.5, 5 ng/mL was between 99.1% and 106.7%. Conclusion The anti-SP-10 paired monoclonal antibody prepared by this method can provide the antibody material basis for the establishment of spermatozoa protein SP-10 immune detection system.
description: Objective To prepare specificity recognize different epitopes of SP-10 couple monoclonal antibodies.Methods Balb/C mice were immunized with SP-10, and screened secret anti-SP-10 monoclonal antibody hybridoma by High Throughput Screening ELISA（HTS-ELISA）and prepared monoclonal antibodies. Then HRP labeling was used to analyze the characteristics of monoclonal antibodies recognizing different epitopes of SP-10. Results Three hybridoma cell lines were screened with monoclonal antibodies secreting specific recognition of SP-10 protein. The monoclonal antibodies secreted by them were named mAb80, mAb81 and mAb208, in which the titer of mAb80 and mAb81 was more than 1:1×107 and the titer of mAb208 was 1:2×106, and the epitopes of mAb208 recognizing SP-10 were significantly different from those of mAb80 and mAb81, showing that they were couple monoclonal antibodies. A sandwich ELISA for the detection of SP-10 was constructed by using mAb208 labeled HRP as the detection antibody and mAb81 as the capture antibody. The minimum detection limit of SP-10 was up to 0.01 ng/mL, the linear range was 0.01-10 ng/mL, and the ELISA recovery of SP-10 with 0.05, 0.5, 5 ng/mL was between 99.1% and 106.7%. Conclusion The anti-SP-10 paired monoclonal antibody prepared by this method can provide the antibody material basis for the establishment of spermatozoa protein SP-10 immune detection system.
DOI: 10.57760/sciencedb.15345
title: Design and Validation of a ‘‘holistic’’ test to Evaluate the Execution Time and Decision-Making in Technical Football Actions
title: Design and Validation of a ‘‘holistic’’ test to Evaluate the Execution Time and Decision-Making in Technical Football Actions

description: This study was evaluated the reliability and validity of a holistic test that assesses soccer skills and performance in a dual-task (DT) context in young players (Soccer Skills and Cognitive Aptitude Test [SoSCAT]). 
description: This study was evaluated the reliability and validity of a holistic test that assesses soccer skills and performance in a dual-task (DT) context in young players (Soccer Skills and Cognitive Aptitude Test [SoSCAT]).