cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8031308
title: Fractions of Selaginella convoluta (Arn.) Spring (Selaginellaceae) attenuate the nociceptive behavior events in mice

description: Abstract Selaginella convoluta (Arn.) Spring is a species popularly known as “jericó”, and used in folk medicine as analgesic and anti-inflammatory. This study aimed to investigate in mice the antinociceptive and anti-inflammatory activities of the hexane (Sc-Hex) and chloroform (Sc-CHCl3) fractions (100, 200 and 400 mg/kg) obtained by partition of crude ethanol extract from S. convoluta. The preliminary phytochemical analysis of the fractions was performed. Antinociceptive activity was evaluated by writhing, formalin and hot-plate tests. Anti-inflammatory activity was evaluated using carrageenan-induced pleurisy. The rota-rod test was used to evaluate motor coordination. Preliminary phytochemical screening showed that the Sc-Hex and the Sc-CHCl3 were positive for the presence of flavonoids, anthracene derivatives, quinones, triterpenes and steroids. Inhibition of writhing was observed for fractions tested. The Sc-Hex at all doses tested was effective in reducing the nociceptive behavior produced by formalin only in the second phase. However, the Sc-CHCl3 decreased the paw licking time in the first and second phases. In the hot plate no significant effect was observed for any fraction. In the rota-rod test, treated mice did not demonstrate any significant motor performance changes. In the carrageenan-induced pleurisy, Sc-CHCl3 (200 mg/kg) reduced cell migration to the pleural cavity. These results reveal the antinociceptive properties of S. convoluta , which support, in part, its traditional use, since the fractions did not presented significant activity in the inflammatory response profile. We further verify that this antinociceptive effect could be by activation of nociceptive peripheral pathway.
DOI: 10.6084/m9.figshare.8031317.v1
title: Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil

description: ABSTRACT Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S. Typhimurium, S. Bredeney, S. Schwarzengrund and S. Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopEand lpfA were selected as possible markers of Salmonella serotypes.
DOI: 10.6084/m9.figshare.8031302.v1
title: ONLINE PRESENCE AS AN ELEMENT OF ANALYSIS OF THE SCHOOL/COMUNITY RELATION. THE UNIDADES EDUCATIVAS DEL MILENIO CASE ON TWITTER

description: ABSTRACT: Presentiality, originally tangible and uni-located, now has an asynchronous and multi-situated character. People and groups create complex presentialities, inhabiting online and offline social spaces in a continuum without sutures. The consideration of spatiality as an integrated dimension is a long-standing epistemological position. The objective of this article is to explore how the Unidades Educativas del Milenio - UEM build their Twitter presentiality to link with the community. We used qualitative, descriptive and analytical method based on analysis of social networks to analyze the Twitter accounts directly referred to these institutions and, later, the network maps generated by the UEM were described as an object of dialogue. Results show that only 11 out of 97 UEM have Twitter accounts, of those, only two have a frequent activity. Additionally, the UEM generate atomized spaces of discussion, and its mentions are conjunctural and mainly referred to reports from state entities.
DOI: 10.6084/m9.figshare.8031308.v1
title: Fractions of Selaginella convoluta (Arn.) Spring (Selaginellaceae) attenuate the nociceptive behavior events in mice

description: Abstract Selaginella convoluta (Arn.) Spring is a species popularly known as “jericó”, and used in folk medicine as analgesic and anti-inflammatory. This study aimed to investigate in mice the antinociceptive and anti-inflammatory activities of the hexane (Sc-Hex) and chloroform (Sc-CHCl3) fractions (100, 200 and 400 mg/kg) obtained by partition of crude ethanol extract from S. convoluta. The preliminary phytochemical analysis of the fractions was performed. Antinociceptive activity was evaluated by writhing, formalin and hot-plate tests. Anti-inflammatory activity was evaluated using carrageenan-induced pleurisy. The rota-rod test was used to evaluate motor coordination. Preliminary phytochemical screening showed that the Sc-Hex and the Sc-CHCl3 were positive for the presence of flavonoids, anthracene derivatives, quinones, triterpenes and steroids. Inhibition of writhing was observed for fractions tested. The Sc-Hex at all doses tested was effective in reducing the nociceptive behavior produced by formalin only in the second phase. However, the Sc-CHCl3 decreased the paw licking time in the first and second phases. In the hot plate no significant effect was observed for any fraction. In the rota-rod test, treated mice did not demonstrate any significant motor performance changes. In the carrageenan-induced pleurisy, Sc-CHCl3 (200 mg/kg) reduced cell migration to the pleural cavity. These results reveal the antinociceptive properties of S. convoluta , which support, in part, its traditional use, since the fractions did not presented significant activity in the inflammatory response profile. We further verify that this antinociceptive effect could be by activation of nociceptive peripheral pathway.
DOI: 10.6084/m9.figshare.8031314.v1
title: Reaction of wild Solanaceae rootstocks to the parasitism of (Meloidogyne javanica)

description: ABSTRACT Intensive production of vegetables in greenhouses can increase the amount of inoculum of soil-borne pathogens, such as the root-knot nematode. Thus, in this study we aimed to evaluate the potential of Solanaceae as rootstocks resistant to Meloidogyne javanica nematodes as an alternative to tomato grafting. The experiment consisted of seven treatments: wild species joá-vermelho (Solanum capsicoides), joá-bagudo (Solanum palinacanthum), joá-bravo (Solanum viarum), jurubeba (Solanum spp.) and the commercial tomato cultivars Santa Cruz Kada, Batalha and Guardião. The analyzed variables were gall index; egg mass index; final nematode population; reproduction factor (FR) and reaction: susceptibility, resistance and immunity; fresh shoot and root mass and number of eggs per gram of roots. The wild species joá-vermelho, joá-bagudo and jurubeba showed resistance, with the lowest indexes of galls, egg mass, final population of nematodes and number of eggs per gram of root, not differing from the resistant control treatment (hybrid rootstock Guardião), with a reproduction factor less than 1, showing potential to be used as a resistant rootstock to M. javanica. Joá-bravo species showed susceptibility to the root-knot nematode, with a FR&gt;1, not differing from the susceptible tomato Santa Cruz Kada. These results confirm the resistance of wild species to nematode parasitism, which can prove the viability of use as possible alternative rootstocks, and reinforce the idea that more studies should be carried out aiming to provide more viable options for farmers and plantlet producers.
DOI: 10.6084/m9.figshare.8031293
title: Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

description: Abstract This study evaluated the antibacterial and antiproliferative activities of the essential oil of Psidium guajava leaves (PG-EO), traditionally used in folk medicine. The essential oil was obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. The major PG-EO chemical constituents were identified by GC-MS and GC-FID as being β-caryophyllene (16.1%), α-humulene (11.9%), aromadendrene oxide (14.7%), δ-selinene (13.6%), and selin-11-en-4α-ol (12.5%). The antibacterial activity of the essential oil of P. guajava leaves was determined in terms of its minimum inhibitory concentrations (MIC) using the broth microdilution method in 96-well microplates. PG-EO had moderate activity against Streptococcus mutans (MIC = 200 µg/mL), S. mitis (MIC = 200 µg/mL), S. sanguinis (MIC = 400 µg/mL), S. sobrinus (MIC = 100 µg/mL), and S. salivarius (MIC = 200 µg/mL). The antiproliferative activity was evaluated against different tumor cell lines: breast adenocarcinoma (MCF-7), human cervical adenocarcinoma (HeLa), and human gliobastoma (M059J). A normal human cell line (GM07492A, lung fibroblasts) was included. The antiproliferative activity was evaluated using the XTT assay and the results were expressed as IC50. The essential oil showed significantly lower IC50 values against MCF-7 and M059J lines than that obtained for the normal line, showing selectivity. Our results suggest that the essential oil of Psidium guajava L. has promising biological activities and can be considered a new source of bioactive compounds.
DOI: 10.6084/m9.figshare.8031296
title: In silico prediction of the functional and structural consequences of the non-synonymous single nucleotide polymorphism A122V in bovine CXC chemokine receptor type 1

description: Abstract The current study aimed to assess whether the A122V causal polymorphism promotes alterations in the functional and structural proprieties of the CXC chemokine receptor type 1 protein (CXCR1) of cattle Bos taurus by in silico analyses. Two amino acid sequences of bovine CXCR1 was selected from database UniProtKB/Swiss-Prot: a) non-polymorphic sequence (A7KWG0) with alanine (A) at position 122, and b) polymorphic sequence harboring the A122V polymorphism, substituting alanine by valine (V) at same position. CXCR1 sequences were submitted as input to different Bioinformatics’ tools to examine the effects of this polymorphism on functional and structural stabilities, to predict eventual alterations in the 3-D structural modeling, and to estimate the quality and accuracy of the predictive models. The A122V polymorphism exerted tolerable and non-deleterious effects on the polymorphic CXCR1, and the predictive structural model for polymorphic CXCR1 revealed an alpha helix spatial structure typical of a receptor transmembrane polypeptide. Although higher variations in the distances between pairs of amino acid residues at target-positions are detected in the polymorphic CXCR1 protein, more than 97% of the amino acid residues in both models were located in favored and allowed conformational regions in Ramachandran plots. Evidences has supported that the A122V polymorphism in the CXCR1 protein is associated with increased clinical mastitis incidence in dairy cows. Thus, the findings described herein prove that the replacement of the alanine by valine amino acids provokes local conformational changes in the A122V-harboring CXCR1 protein, which could directly affect its post-translational folding mechanisms and biological functionality.
DOI: 10.6084/m9.figshare.8031293.v1
title: Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

description: Abstract This study evaluated the antibacterial and antiproliferative activities of the essential oil of Psidium guajava leaves (PG-EO), traditionally used in folk medicine. The essential oil was obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. The major PG-EO chemical constituents were identified by GC-MS and GC-FID as being β-caryophyllene (16.1%), α-humulene (11.9%), aromadendrene oxide (14.7%), δ-selinene (13.6%), and selin-11-en-4α-ol (12.5%). The antibacterial activity of the essential oil of P. guajava leaves was determined in terms of its minimum inhibitory concentrations (MIC) using the broth microdilution method in 96-well microplates. PG-EO had moderate activity against Streptococcus mutans (MIC = 200 µg/mL), S. mitis (MIC = 200 µg/mL), S. sanguinis (MIC = 400 µg/mL), S. sobrinus (MIC = 100 µg/mL), and S. salivarius (MIC = 200 µg/mL). The antiproliferative activity was evaluated against different tumor cell lines: breast adenocarcinoma (MCF-7), human cervical adenocarcinoma (HeLa), and human gliobastoma (M059J). A normal human cell line (GM07492A, lung fibroblasts) was included. The antiproliferative activity was evaluated using the XTT assay and the results were expressed as IC50. The essential oil showed significantly lower IC50 values against MCF-7 and M059J lines than that obtained for the normal line, showing selectivity. Our results suggest that the essential oil of Psidium guajava L. has promising biological activities and can be considered a new source of bioactive compounds.
DOI: 10.6084/m9.figshare.8031296.v1
title: In silico prediction of the functional and structural consequences of the non-synonymous single nucleotide polymorphism A122V in bovine CXC chemokine receptor type 1

description: Abstract The current study aimed to assess whether the A122V causal polymorphism promotes alterations in the functional and structural proprieties of the CXC chemokine receptor type 1 protein (CXCR1) of cattle Bos taurus by in silico analyses. Two amino acid sequences of bovine CXCR1 was selected from database UniProtKB/Swiss-Prot: a) non-polymorphic sequence (A7KWG0) with alanine (A) at position 122, and b) polymorphic sequence harboring the A122V polymorphism, substituting alanine by valine (V) at same position. CXCR1 sequences were submitted as input to different Bioinformatics’ tools to examine the effects of this polymorphism on functional and structural stabilities, to predict eventual alterations in the 3-D structural modeling, and to estimate the quality and accuracy of the predictive models. The A122V polymorphism exerted tolerable and non-deleterious effects on the polymorphic CXCR1, and the predictive structural model for polymorphic CXCR1 revealed an alpha helix spatial structure typical of a receptor transmembrane polypeptide. Although higher variations in the distances between pairs of amino acid residues at target-positions are detected in the polymorphic CXCR1 protein, more than 97% of the amino acid residues in both models were located in favored and allowed conformational regions in Ramachandran plots. Evidences has supported that the A122V polymorphism in the CXCR1 protein is associated with increased clinical mastitis incidence in dairy cows. Thus, the findings described herein prove that the replacement of the alanine by valine amino acids provokes local conformational changes in the A122V-harboring CXCR1 protein, which could directly affect its post-translational folding mechanisms and biological functionality.
DOI: 10.6084/m9.figshare.8031278
title: The parting of the seas: norms, material power and state control over the ocean

description: Abstract The paper argues that the norm of sovereignty was extended to sea areas with only minor adaptations. Using an English School approach, it explores the political evolution of control over the seas, demonstrating why and how the norm of sovereignty prevailed over alternative norms and principles regulating control of the seas. The paper then compares the positions of Brazil, China and the United States on the current international regime of the ocean.
DOI: 10.6084/m9.figshare.8031284
title: Between the Sensible and the Intelligible: A Semiotic Reading of the Episode The National Anthem of the Series Black Mirror

description: ABSTRACT This paper proposes an articulation between the theoretical models of regimes of interaction and meaning and regimes of visibility. Complementarily, it also mobilizes the concepts of union and contagion - part of Eric Landowski's sociosemiotics, aiming at understanding the relationships between the sensible and the intelligible in the engenderment of meaning in the episode The National Anthem of the British series Black Mirror. This proposition proves thereby to be fruitful, since it allows demonstrating the overlaps and passages from a regime based on the intelligible (manipulation) to a regime based on the sensible (adjustment). Based on the syntax of seeing, it introduces a new regime of interaction through the various possibilities between wanting-to-see and wanting-to-be-seen. Finally, the paper also poses the problem of the point of view regarding accident and programming. It is possible to observe that the presence of the spectator, at a given point in the syntagmatic chain, that is, being-able-to-see or being-able-not-to-see as regards these two regimes, can make emerge either one of the regimes of interaction and meaning, which are characterized, respectively, by the sensible and the intelligible.
DOI: 10.6084/m9.figshare.8031287
title: The social network of adolescents who need special health care

description: ABSTRACT Objective: To describe the social network of adolescents who need special health care. Method: A qualitative, descriptive and exploratory study conducted between 2016 and 2017 in the pediatric outpatient clinic of a teaching hospital in southern Brazil. Thirty-five semi-structured interviews were conducted with adolescents between 12 and 18 years of age, followed by the construction of genograms and ecomaps. After transcription, the enunciations were subjected to Pêcheux’s method of discourse analysis. Results: The institutional network consists of health services, schools and religious entities, as well as adolescents’ families and friends. In the family network, women family members—such as mothers, grandmothers and aunts—have a special role. Final Consideration: The adolescents’ social network is composed of institutional and family circles. In the view of adolescents, the tertiary service is more capable of solving their problems, and for this reason used the most. In order to ensure these adolescents access to and continuity of care, the articulation between health policies and health services is suggested.
DOI: 10.6084/m9.figshare.8031275
title: Strategies for preventing ventilator-associated pneumonia: an integrative review

description: ABSTRACT Objective: to identify studies about strategies for prevention of ventilator-associated pneumonia deployed in health services and classify their level of evidence. Method: integrative review of the literature, in 7 databases, which included the following descriptors: Prevention and Control AND Pneumonia Ventilator-Associated AND Intensive Care Units AND Bundle AND Patient Care. Results: twenty-three scientific productions were included. Of the preventive measures identified, 9 (39.1%) correspond from three to five strategies. The most frequent were: 22 (95.6%) lying with head elevated, 19 (82.6%) oral hygiene with chlorhexidine and 14 (60.8%) reduction of sedation whenever possible. Final Consideration: the application of measures based on scientific evidence is proven to be effective when carried out in conjunction, impacting the reduction of the incidence of ventilator-associated pneumonia.
DOI: 10.6084/m9.figshare.8031284.v1
title: Between the Sensible and the Intelligible: A Semiotic Reading of the Episode The National Anthem of the Series Black Mirror

description: ABSTRACT This paper proposes an articulation between the theoretical models of regimes of interaction and meaning and regimes of visibility. Complementarily, it also mobilizes the concepts of union and contagion - part of Eric Landowski's sociosemiotics, aiming at understanding the relationships between the sensible and the intelligible in the engenderment of meaning in the episode The National Anthem of the British series Black Mirror. This proposition proves thereby to be fruitful, since it allows demonstrating the overlaps and passages from a regime based on the intelligible (manipulation) to a regime based on the sensible (adjustment). Based on the syntax of seeing, it introduces a new regime of interaction through the various possibilities between wanting-to-see and wanting-to-be-seen. Finally, the paper also poses the problem of the point of view regarding accident and programming. It is possible to observe that the presence of the spectator, at a given point in the syntagmatic chain, that is, being-able-to-see or being-able-not-to-see as regards these two regimes, can make emerge either one of the regimes of interaction and meaning, which are characterized, respectively, by the sensible and the intelligible.
DOI: 10.6084/m9.figshare.8031287.v1
title: The social network of adolescents who need special health care

description: ABSTRACT Objective: To describe the social network of adolescents who need special health care. Method: A qualitative, descriptive and exploratory study conducted between 2016 and 2017 in the pediatric outpatient clinic of a teaching hospital in southern Brazil. Thirty-five semi-structured interviews were conducted with adolescents between 12 and 18 years of age, followed by the construction of genograms and ecomaps. After transcription, the enunciations were subjected to Pêcheux’s method of discourse analysis. Results: The institutional network consists of health services, schools and religious entities, as well as adolescents’ families and friends. In the family network, women family members—such as mothers, grandmothers and aunts—have a special role. Final Consideration: The adolescents’ social network is composed of institutional and family circles. In the view of adolescents, the tertiary service is more capable of solving their problems, and for this reason used the most. In order to ensure these adolescents access to and continuity of care, the articulation between health policies and health services is suggested.
DOI: 10.6084/m9.figshare.8031275.v1
title: Strategies for preventing ventilator-associated pneumonia: an integrative review

description: ABSTRACT Objective: to identify studies about strategies for prevention of ventilator-associated pneumonia deployed in health services and classify their level of evidence. Method: integrative review of the literature, in 7 databases, which included the following descriptors: Prevention and Control AND Pneumonia Ventilator-Associated AND Intensive Care Units AND Bundle AND Patient Care. Results: twenty-three scientific productions were included. Of the preventive measures identified, 9 (39.1%) correspond from three to five strategies. The most frequent were: 22 (95.6%) lying with head elevated, 19 (82.6%) oral hygiene with chlorhexidine and 14 (60.8%) reduction of sedation whenever possible. Final Consideration: the application of measures based on scientific evidence is proven to be effective when carried out in conjunction, impacting the reduction of the incidence of ventilator-associated pneumonia.