DOI: 10.5061/dryad.3t2d4
title: Data from: Wood-inhabiting insects can function as targeted vectors for decomposer fungi

description: Most wood-inhabiting fungi are assumed to be dispersed primarily by wind,
 with the exception of a few species involved in mutualistic relationships
 with insects. In this study we tested whether several species of
 wood-inhabiting insects can function as dispersal vectors for
 non-mutualistic fungi, which would indicate that wood-inhabiting fungi can
 benefit from targeted animal-mediated dispersal. We sampled
 wood-inhabiting beetles (Coleoptera) from freshly felled wood
 experimentally added to forests and used DNA metabarcoding to investigate
 the fungal DNA carried by these insects. Staphylinid beetles rarely
 contained fungal DNA, while Endomychus coccineus, Glischrochilus hortensis
 and Glischrochilus quadripunctatus frequently carried fungal DNA with a
 composition specific to the insect taxon. A large proportion of the
 obtained fungal sequences (34%) represented decomposer fungi, including
 well-known wood-decay fungi such as Fomitopsis pinicola, Fomes
 fomentarius, Trichaptum abietinum and Trametes versicolor. Scanning
 electron microscopy further showed that some of the fungal material was
 carried as spores or yeast cells on the insect exoskeletons. Our results
 suggest that insect-vectored dispersal is of broader importance to
 wood-inhabiting fungi than previously assumed.
description: Jacobsen_Pool1_forward_readsBzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA extracted from 103 samples (Pool1) of wood-inhabiting insects.NG-9365_Rannveig1_lib117480_4476_2_1.fastq.bz2Jacobsen_Pool1_backward_readsBzip-compressed fastq-file containing raw sequences (backward reads) from Illumina HiSeq of pooled fungal DNA extracted from 103 samples (Pool1) of wood-inhabiting insects.NG-9365_Rannveig1_lib117480_4476_2_2.fastq.bz2Jacobsen_mapping_file_DNAExcel-file with tag/mid (molecular identifier) sequences, ITS-sequences, sample IDs for tags/mids and lists of repetitions and negatives.Jacobsen_OTU_table_Fungal_EcologyOTU-table with taxonomic annotation for OTUs and information on insect samples.Jacobsen_Pool2_forward_reads_part1First half of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA extracted from 98 samples (Pool2) of wood-inhabiting insects. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).NG-9365_Rannveig2_lib117481_4476_2_1.fastq.bz2.001Jacobsen_Pool2_forward_reads_part2Second half of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA extracted from 98 samples (Pool2) of wood-inhabiting insects. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).NG-9365_Rannveig2_lib117481_4476_2_1.fastq.bz2.002Jacobsen_Pool2_backward_reads_part1First half of the bzip-compressed fastq-file containing raw sequences (backward reads) from Illumina HiSeq of pooled fungal DNA extracted from 98 samples (Pool2) of wood-inhabiting insects. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).NG-9365_Rannveig2_lib117481_4476_2_2.fastq.bz2.001Jacobsen_Pool2_backward_reads_part2Second half of the bzip-compressed fastq-file containing raw sequences (backward reads) from Illumina HiSeq of pooled fungal DNA extracted from 98 samples (Pool2) of wood-inhabiting insects. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).NG-9365_Rannveig2_lib117481_4476_2_2.fastq.bz2.002Rep_seqs_OTUs_scata_10kRepresentative sequences for OTUs as clustered by Scata (https://scata.mykopat.slu.se/) in subsampled dataset (max 10k sequences from each insect sample).Rep_seqs_clusters_scata_10k.fas