DOI: 10.5061/dryad.h9w0vt4rf
title: Sticky card color and luminance on insect capture

description: Coloured sticky traps are commonly used to monitor insect pests. Colour
 affects trap performance; with preferred colours often differing between
 species, making selection of trap colour for effective management of
 multiple pests challenging. Greenhouse whitefly (GWF) Trialeurodes
 vaporariorum and Western flower thrips (WFT) Frankliniella occidentalis,
 are major horticultural pests that often co-occur. Yellow colours are
 attractive to GWF, while blue is often used to target WFT, although WFT
 are also attracted to yellow colours in certain contexts. The visual
 properties that make yellow colours attractive to either species are not
 well understood. Previous experiments in WFT find that visual modelling of
 an opponent mechanism between short wavelength sensitive (SWS) and long
 wavelength sensitive (LWS) photoreceptors optimises performance of blue
 sticky traps. In the current study, we assess whether an opponent response
 that highly stimulates LWS relative to SWS photoreceptors predicts the
 attractiveness of yellow sticky cards to both WFT and GWF. Our results
 show that yellow sticky cards that maximize a predicted SWS:LWS opponent
 mechanism improves capture for both species. Further optimizing the
 SWS:LWS ratio allowed for simultaneous monitoring of both pest species
 using single colour cards. We also show that sticky trap colour and
 luminance are comparable across different lab and field contexts,
 highlighting the broad applicability of visual modelling in pest
 management strategies.
description: # Insect catch raw data and code
 [https://doi.org/10.5061/dryad.h9w0vt4rf](https://doi.org/10.5061/dryad.h9w0vt4rf) ## Description of the data and file structure * Raw data files (.csv) * brightvlum_yellow = greenhouse whitefly (GWF) and western flower thrips (WFT) caught on traps for experiment 1 in the associated manuscript * Colour:  categorical classification of yellow colors tested (Y11, Y3, Y5) * Tunnel: Polytunnel replicates for experimental testing * Row: Crop row replicates within each polytunnel * pLum: predicted luminance of each color for the insect visual system * pBG: predicted BG ratio of each color for the insect visual system * mBG: measured BG ratio from photographs of cards taken in polytunnels * mLum: measured luminance from photographs of cards taken in polytunnels * Species: western flower thrips (WFT) or greenhouse whitefly (GWF) * Count: number of insects caught * Solids = GWF and WFT caught on traps for experiment 2 in the associated manuscript. * Colour:  categorical classification of yellow colors tested (B5, Y162, Y193, Y195, Y199, 7205) * Tunnel: Polytunnel replicates for experimental testing * Row: Crop row replicates within each polytunnel * Species: western flower thrips (WFT) or greenhouse whitefly (GWF) * Lum: luminance of each color for the insect visual system * BG: BG ratio of each color for the insect visual system * Count: number of insects caught * Lumnorm: luminance of each color for the insect visual system normalized against the maximum Lum measurement * BGnorm: BG ratio of each color for the insect visual system normalized against the maximum BG measurement * Image Analysis GWF_check 1 = quantal catches in response to cards from experiment 1 for GWF, calculated using micatoolbox and Image J . All missing data represented as na. * Tunnel: Polytunnel replicate * Colour: Categorical sticky card color tested * ID: card ID * ROI: region of interest measured within each card ID * lumMean: mean luminance for ROIs ("na" when luminance was not measured) * lumSD: standard deviation of luminance for each ROI ("na" when luminance was not measured) * Area: area of each ROI (pixels) ("na" when luminance was not recorded) * lwMean: long wave receptor quantal catch mean * lwSD: long wave receptor quantal catch standard deviation * swMean: short wave receptor quantal catch mean * swSD: short wave receptor quantal catch standard deviation * UVMean: ultraviolet wave receptor quantal catch mean * UVSD: ultraviolet wave receptor quantal catch standard deviation * BG: blue-green ratio * Image Analysis WFT_check 1 = quantal catches in response to cards from experiment 1 for WFT, calculated using micatoolbox and Image J * column labels same as for Image Analysis GWF_check 1 (but for WFT visual system) * Analysis files (.R) * Final_Yellow_analysis = R code associated with analyses for experiment 1 * Final_Multi_analysis = R code associated with analyses for experiment 2 ## Code/Software Analysis in R (ver 4.3.2). Associated libraries for analysis are available in the provided code.