DOI: 10.5061/dryad.r058p
title: Data from: Variation at phenological candidate genes correlates with timing of dispersal and plumage morph in a sedentary bird of prey

description: Polymorphic genes involved in the conserved molecular signalling of
 circadian and circannual clocks may play important roles in governing the
 timing of breeding and dispersal and thereby affect fitness in
 vertebrates. However, relatively few studies have explored associations
 between phenological candidate genes and behaviour, and these are somewhat
 biased towards particular taxonomic groups such as passerine birds and
 salmonid fish. Consequently, we assayed microsatellite polymorphisms
 within the exonic and 3’ untranslated regions of the regulatory genes
 CLOCK, NPAS2, ADCYAP1 and CREB1 in the common buzzard (Buteo buteo), a
 polymorphic raptor species with three plumage morphs that differ in key
 life history traits including lifetime reproductive success. In contrast
 to studies of passerines, CLOCK poly-glutamine (poly-Q) was found to be
 monomorphic in 976 common buzzard nestlings as well as in three other
 Buteo species. Moreover, none of the candidate genes were significantly
 associated with fledging dates, although intermediately melanised females
 were found to lay earlier on average than light or dark morph individuals,
 and their offspring carried longer ADCYAP1 alleles. In contrast, all three
 candidate genes explained significant variation in one or more measures of
 juvenile buzzard dispersal (resighting probability, timing of dispersal
 and distance dispersed). Our findings contribute towards a broader body of
 work on the adaptive significance of CLOCK polymorphism, while also
 building upon previous studies that have documented links between ADCYAP1
 variability and the timing of migration.
description: Common buzzard broods
 with parental identity and morph and timing dataData on common buzzard
 (Buteo buteo) breeding collected in the field close to Bielefeld, North
 Rhine-Westphalia. Brood attempts contain data on fledging date and
 parental identities and melanin morphs.broods.csvGenotyped nestlings with melanin morph, parental identities and morphs and brood timingGenotyped for NPAS2 poly-Q, ADCYAP1 and CREB1nestlings.csvData on common buzzard resightings after being ringed as nestlingsIncludes distance from natal nest, genotypes for ADCYAP1, CREB1, NPAS2 own and parental melanin morphsresighted.csvDataset of juvenile buzzards with identified dispersal behaviourContaining for each individual: distance from natal nest and days since ringing, ADCYAP1, CREB1 and NPAS2 genotypes, melanin morphs of nestlings and offspring.dispersers.csvR code for analysis of data files as performed by Chakarov et al.R code for analysis of associations between parental identity, parental melanin morph and brood timing, buzzard ADCYAP1, CREB1 and NPAS2 genotype and association with timing (of own hatching) and own dispersal behavior.code_Chakarovetal_phenological candidate genes.RSequences of CLOCK, NPAS2, ADCYAP1 and CREB1 of FalconiformesEdited and aligned sequences of CLOCK, NPAS2, ADCYAP1 and CREB1 repeat alleles and flanking regions of phenological candidate gene from Buteo, Accipiter, Milvus, Pernis and Falco.sequencesChakarovetal_clock_adcyap1_creb1_npas2.gb