DOI: 10.5061/dryad.n8pk0p2zp
title: Data from: Development of a 3D simulator for training the mouse in utero electroporation

description: In utero electroporation (IUE) requires high-level training in
 microinjection through the mouse uterine wall into the lateral ventricle
 of the mouse brain. Training for IUE is currently being performed in live
 mice, as no artificial models allow simulations yet. This study
 aimed to develop an anatomically realistic 3D-printed simulator to train
 IUE in mice.  To this end, we created embryo models containing
 lateral ventricles. We coupled them to uterus models in six steps: (1)
 computed tomography imaging, (2) 3D model segmentation, (3) 3D model
 refinement, (4) mold creation to cast the actual model, (5) 3D mold
 printing, and (6) mold casting the molds with a mix of soft silicones to
 ensure the hardness and consistency of the uterus and embryo. The results
 showed that the simulator assembly successfully recreated the IUE. The
 compression test did not differ in the mechanical properties of the real
 embryo or in the required load for uterus displacement. Furthermore, more
 than 90% of the users approved the simulator as an introduction to IUE and
 considered that the simulator could help reduce the number of animals for
 training. Despite current limitations, our 3D simulator enabled a
 realistic experience for initial approximations to the IUE and is a real
 alternative for implementing the 3Rs. We are currently working on refining
 the model.
description: # Development of a 3D simulator for training the mouse in utero
 electroporation
 [https://doi.org/10.5061/dryad.n8pk0p2zp](https://doi.org/10.5061/dryad.n8pk0p2zp) ## Description of the data and file structure A three-part mold for the uterus model was created, consisting of two outer parts for the exterior shape and an inner part representing the space for the embryos. The inner part had a tab to hold the silicone outer mold with a 0.5 mm gap. Cylinders and a cube from the Netfabb library were combined using the "Boolean union" function. The inner model was hollowed with a 0.5 mm offset, then combined with the cube. The outer model was subtracted from the outer box and separated using the "plane cut" function. The details of the experimental design can be found in the corresponding publication. The data incluce: 1. Mechanical properties from embryos and their models 2. Mechanical properties from uteri and their models 3. Data of the survey evaluating of the IUE simulator ### Files and variables #### File: Data\_IUE\_Model\_Survey.csv The IUE simulation model was evaluated through a practical session with a questionnaire at the University Medical Center's IUE facility. Users, classified as beginner/intermediate (n = 3) and expert (n = 3), practiced IUE surgery steps on the models and provided feedback on using this model as an alternative to live-animal training. **Description:** User evaluation (%) to each of our questions to evaluate the IUE simulator ##### Questions * Model rating overall * Model rating for introduction * Model rating practicing steps * Model rating for replacement * Model rating for reduction #### File: Data\_IUE\_Model\_Uterus.csv Mice were sacrificed at E15, and their uteri were incised at both ends. Embryos were extracted carefully to avoid uterine damage. Surgical-grade thread was used to suture loops around the uterus, maintaining a 5 mm distance. The uterus was positioned on hooks of a force test device, which was tared to zero and set to move at 10 mm/min with a minimal force of 0.002 N. The device moved to a maximum of 30 mm while measuring the force increase. **Description:** Load [N] per displacement [mm] as a measure of hardness ##### Variables * Uterus (E15) * Uterus silicone model (SH00-20) * Uterus silicone model (SH00-30) #### File: Data\_IUE\_Model\_Embryos.csv Shore00-10, 20, and 30 silicones were mixed, colored, and degassed before being poured into the mold. The upper head part was filled first, followed by the main body. The molds were closed with clamps, and silicones were allowed to harden overnight. Only Sh00-30 models were intact after demolding; others were destroyed. Silicone was prepared without coloring, poured into the mold, and the inner mold part inserted. The mold was clamped and left to harden overnight. The next day, the model was removed gently. Sh00-20 and Sh00-30 silicones were used for their similar elasticity to the embryo, with Sh00-30 selected for its easier demolding and handling properties. **Description:** Load [N] per displacement [mm] as a measure of hardness ##### Variables * Embryo * Embryo silicone model