cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7743224.v1
title: Potassium Fertilization of Eucalyptus in an Entisol in Low-Elevation Cerrado

description: ABSTRACT Potassium (K) is one of the most highly accumulating nutrients in Eucalyptus and, consequently, is heavily exported by the harvesting of wood. Moreover, its availability in the soil in most Brazilian plantation areas is very low, especially in the regions of the Cerrado biome, which has soils with low natural fertility and marked water deficits, implying a lack of nutrient supply and, consequently, a less efficient water use. Our objective was to evaluate the effects of K fertilization on Eucalyptus biomass yield, the addition of nutrients to the soil by leaf deposition, nutrient use efficiency, and soil K availability. The experiment was conducted with clone I144 (Eucalyptus urophylla) in the municipality of Três Lagoas/MS, in a Neossolo Quartzarênico Órtico (Entisol). The experimental design was a randomized block with four treatments and five replicates. The treatments consisted of four K doses (0, 90, 135, and 180 kg ha-1 of K2O) as KCl. Plant biomass production (leaves, branches, trunk, and bark), senescent leaf deposition, leaf nutrient concentrations, nutrient accumulation in the different plant compartments, nutrient use efficiency, addition of nutrients to the soil by leaf deposition, and soil K availability were evaluated. Potassium fertilization increased the biomass yield of Eucalyptus plants, senescent leaf K content, the transfer of K to the soil, the accumulation of K in the aerial plant parts, and the K content in the soil. However, it did not influence senescent leaf deposition yield or plant K use efficiency.
DOI: 10.7284/106654
title: GNSS (Global Navigation Satellite System) data collected during research cruise AMAT05RR using a Furuno GP-90D instrument system onboard the platform RV Roger Revelle

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743206
title: Grain Yield Losses and Economic Threshold Level Of GR® F2 Volunteer Corn in Cultivated F1 Hybrid Corn

description: ABSTRACT: Volunteer corn is competitive with cultivated corn and the degree of interference varies according to their density and origin. This study aimed to determine the grain yield losses of GR® F1 hybrid corn as a function of the interferences with GR® F2 volunteer corn densities from individual plants and clumps, and determine the of economic threshold (ET). Two experiments were carried out in the field in a randomized block design with four replications and eight volunteer corn densities between 0 and 12 individual plants or clumps m-2. Each clump consisted of seven corn plants adhered to a piece of the rachis in the same point. The rectangular hyperbola model fitted to the percentage of grain yield losses of corn, generating the parameters for determining ET, which was calculated as a function of cost (US$ ha-1) and efficiency (%) of corn control, the price paid for corn (US$ kg-1), and corn grain yield (kg ha-1). Grain yield losses ranged from 2.7% to 34% for densities of 0.5 and 12 individual plants m-2 and 6.1% to 42% for 0.5 and 12 clumps m-2, respectively. The joint analysis of the results of experiments showed that from density of 2 m2 clumps are more competitive than individual plants and causes higher yield losses on cultivated corn. The average ET was 0.44 individual plants m-2 and 0.19 clumps m-2. However, from these densities, the control should be performed. Increases in corn grain yield and price paid, higher corn control efficiency, and a decrease in control costs promote a reduction in ET of volunteer corn in corn.
DOI: 10.6084/m9.figshare.7743209
title: Do Aggregate Size Classes of the Subsurface Soil Horizon Have Different Chemical/Mineralogical Properties?

description: ABSTRACT Variations in chemical and mineralogical properties of a soil can occur at short vertical and horizontal distances. The objective of the present study was to evaluate the chemical and mineralogical soil properties of different aggregate classes from young (Ustrochrept) and highly weathered (Acrustox) soils from the state of Paraná, Brazil. Undisturbed blocks (0.20 ×0.20 ×0.20 m) of soils were separated into aggregate classes: Acrustox – 8.0-4.0 mm; 4-2 mm; 2.0-0.5 mm; 0.5-0.2 mm; and &lt;0.2 mm; and Ustrochrept – 8.0-4.0 mm; 4.0-2.0 mm; 2.0-0.5 mm; and 0.5-0.2 mm. The exchangeable K contents showed an opposite behavior for the two soils: higher contents in the Acrustox for the larger aggregate classes and higher contents in the Ustrochrept for the smaller aggregate classes. The crystallographic characteristics of hematite and goethite were variable according to the aggregate class. The goethite in the 2-4-mm aggregate class is expected to exhibit the highest reactivity for the Ustrochrept: lower growth and a more elongated form of the crystals. The smallest aggregate class presented the lowest contents of kaolinite and gibbsite in the clay fraction. Gibbsite and kaolinite in the intermediate classes presented higher growth in both soils. The studied soils present different side-by-side environments (aggregate classes) for the exploration by the root system. This means that for the precise identification of the environments explored by the roots of a single plant, field sampling should consider the aggregate class, obtained from an undisturbed soil sample. However, this is impractical in agricultural practice.
DOI: 10.6084/m9.figshare.7743215.v1
title: Sugarcane Root Development and Yield under Different Soil Tillage Practices

description: ABSTRACT New strategies for sugarcane production have been very important since the incorporation of ethanol in the Brazilian energy mix in the early 1970s. Prior to planting sugarcane, the soil is prepared, and this process can affect root development and, consequently, sugarcane production. This study was conducted in an area of sugarcane crop renewal in the Cerrado biome (Brazilian tropical savanna), with the objective of identifying which tillage system generates the better root development and improved yield in sugarcane. The treatments were: 1) weed desiccation + moldboard plowing (0.4 m) + mild spike tooth harrowing (0.15 m); 2) subsoiler (0.3 m) + mild spike tooth harrowing (0.15 m); 3) weed desiccation + no-tillage (furrow opening and fertilizer); 4) weed desiccation + subsoiler (0.4 m); 5) ratoon destruction + subsoiler (0.4 m); 6) ratoon destruction + spike tooth harrowing (0.2 m) + moldboard plowing (0.4 m) + mild spike tooth harrowing (0.15 m). Characteristics of the sugarcane root system, such as the root length density, average distance between roots, and root soil exploration, after the first harvest (1.5 years) were studied. Root length density was greater for the treatments that included plowing (0.4 m) and harrowing (0.15 m) operations. The average distance between roots was low in the no-tillage system. The highest sugarcane yield in the plant crop was achieved by management practices with more extensive soil profile disturbances, like plowing followed by harrowing.
DOI: 10.6084/m9.figshare.7743206.v1
title: Grain Yield Losses and Economic Threshold Level Of GR® F2 Volunteer Corn in Cultivated F1 Hybrid Corn

description: ABSTRACT: Volunteer corn is competitive with cultivated corn and the degree of interference varies according to their density and origin. This study aimed to determine the grain yield losses of GR® F1 hybrid corn as a function of the interferences with GR® F2 volunteer corn densities from individual plants and clumps, and determine the of economic threshold (ET). Two experiments were carried out in the field in a randomized block design with four replications and eight volunteer corn densities between 0 and 12 individual plants or clumps m-2. Each clump consisted of seven corn plants adhered to a piece of the rachis in the same point. The rectangular hyperbola model fitted to the percentage of grain yield losses of corn, generating the parameters for determining ET, which was calculated as a function of cost (US$ ha-1) and efficiency (%) of corn control, the price paid for corn (US$ kg-1), and corn grain yield (kg ha-1). Grain yield losses ranged from 2.7% to 34% for densities of 0.5 and 12 individual plants m-2 and 6.1% to 42% for 0.5 and 12 clumps m-2, respectively. The joint analysis of the results of experiments showed that from density of 2 m2 clumps are more competitive than individual plants and causes higher yield losses on cultivated corn. The average ET was 0.44 individual plants m-2 and 0.19 clumps m-2. However, from these densities, the control should be performed. Increases in corn grain yield and price paid, higher corn control efficiency, and a decrease in control costs promote a reduction in ET of volunteer corn in corn.
DOI: 10.6084/m9.figshare.7743209.v1
title: Do Aggregate Size Classes of the Subsurface Soil Horizon Have Different Chemical/Mineralogical Properties?

description: ABSTRACT Variations in chemical and mineralogical properties of a soil can occur at short vertical and horizontal distances. The objective of the present study was to evaluate the chemical and mineralogical soil properties of different aggregate classes from young (Ustrochrept) and highly weathered (Acrustox) soils from the state of Paraná, Brazil. Undisturbed blocks (0.20 ×0.20 ×0.20 m) of soils were separated into aggregate classes: Acrustox – 8.0-4.0 mm; 4-2 mm; 2.0-0.5 mm; 0.5-0.2 mm; and &lt;0.2 mm; and Ustrochrept – 8.0-4.0 mm; 4.0-2.0 mm; 2.0-0.5 mm; and 0.5-0.2 mm. The exchangeable K contents showed an opposite behavior for the two soils: higher contents in the Acrustox for the larger aggregate classes and higher contents in the Ustrochrept for the smaller aggregate classes. The crystallographic characteristics of hematite and goethite were variable according to the aggregate class. The goethite in the 2-4-mm aggregate class is expected to exhibit the highest reactivity for the Ustrochrept: lower growth and a more elongated form of the crystals. The smallest aggregate class presented the lowest contents of kaolinite and gibbsite in the clay fraction. Gibbsite and kaolinite in the intermediate classes presented higher growth in both soils. The studied soils present different side-by-side environments (aggregate classes) for the exploration by the root system. This means that for the precise identification of the environments explored by the roots of a single plant, field sampling should consider the aggregate class, obtained from an undisturbed soil sample. However, this is impractical in agricultural practice.
DOI: 10.7284/106655
title: GNSS (Global Navigation Satellite System) data collected during research cruise AMAT05RR using a Ashtech ADU2 instrument system onboard the platform RV Roger Revelle

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743194
title: Lignin-First Biorefining of Lignocellulose: the Impact of Process Severity on the Uniformity of Lignin Oil Composition

description: In lignin-first biorefining via reductive processes, lignocellulosic materials are deconstructed by the solvent extraction of lignin in the presence of a hydrogenation catalyst. This approach provides a route to the successful extraction and reductive passivation of lignin fragments to produce low molar mass lignin oils together with high-quality pulps. Herein, we present an investigation into the impact of process severity (i.e., cooking temperature) on the reductive processes taking place on the lignin fragments and uniformity of the product mixture. In addition to improving overall delignification yields (up to 87%) and producing low molar mass fragments, higher process temperatures led to the preferential cleavage of hydroxyl groups in monolignol sidechains via hydrodeoxygenation, yielding oils with lower oxygen content. By comparing products from both lignin-first biorefining and organosolv processes at various temperatures, we elucidate key performance differences and outline routes to increased chemical uniformity in lignin streams. Overall, this study outlines clearly the importance of process temperature in the deconstruction of lignocellulose by lignin-first biorefining when producing highly depolymerized lignin products. This study points out a trade-off in the effect of temperature upon delignification and increase in product mixture complexity, which needs to be carefully optimized for the scale-up of lignin-first technologies.
DOI: 10.6084/m9.figshare.7743194.v1
title: Lignin-First Biorefining of Lignocellulose: the Impact of Process Severity on the Uniformity of Lignin Oil Composition

description: In lignin-first biorefining via reductive processes, lignocellulosic materials are deconstructed by the solvent extraction of lignin in the presence of a hydrogenation catalyst. This approach provides a route to the successful extraction and reductive passivation of lignin fragments to produce low molar mass lignin oils together with high-quality pulps. Herein, we present an investigation into the impact of process severity (i.e., cooking temperature) on the reductive processes taking place on the lignin fragments and uniformity of the product mixture. In addition to improving overall delignification yields (up to 87%) and producing low molar mass fragments, higher process temperatures led to the preferential cleavage of hydroxyl groups in monolignol sidechains via hydrodeoxygenation, yielding oils with lower oxygen content. By comparing products from both lignin-first biorefining and organosolv processes at various temperatures, we elucidate key performance differences and outline routes to increased chemical uniformity in lignin streams. Overall, this study outlines clearly the importance of process temperature in the deconstruction of lignocellulose by lignin-first biorefining when producing highly depolymerized lignin products. This study points out a trade-off in the effect of temperature upon delignification and increase in product mixture complexity, which needs to be carefully optimized for the scale-up of lignin-first technologies.
DOI: 10.6084/m9.figshare.7743179
title: IMPROVEMENT IN OXIDATIVE STRESS AFTER DUODENOJEJUNOSTOMY IN AN EXPERIMENTAL MODEL OF TYPE 2 DIABETES MELLITUS

description: ABSTRACT Background: Type 2 Diabetes Mellitus is a multifactorial syndrome with severe complications. Oxidative stress is accepted as a causal factor of chronic complications Aim: To demonstrate alterations in oxidative stress after metabolic surgery. Methods: Twenty-four 2-day-old Wistar rats were used. In 16, Type 2 Diabetes Mellitus was induced by 100 mg/kg streptozotocin injection. The development of diabetes was confirmed after 10 weeks using an oral glucose tolerance test. Eight diabetic rats composed the diabetic surgical group; the remaining eight composed the diabetic group. Eight animals in which diabetes was not induced formed the clinical control group. The Marchesini technique was used in the diabetic surgical group. After 90 days, the rats were sacrificed, and the oxidative stress markers were measured. Results: Thiobarbituric acid reactive substances, superoxide dismutase and catalase were significantly reduced in the diabetic surgical group compared to the diabetic group. Conclusion: The duodenojejunostomy was effective in controlling the exacerbated oxidative stress present in diabetic rats.
DOI: 10.6084/m9.figshare.7743182
title: A lung image reconstruction from computed radiography images as a tool to tuberculosis treatment control

description: Abstract Background: Background: Tuberculosis (TB) is an infectious lung disease with high worldwide incidence that severely compromises the quality of life in affected individuals. Clinical tests are currently employed to monitor pulmonary status and treatment progression. The present study aimed to apply a three-dimensional (3D) reconstruction method based on chest radiography to quantify lung-involvement volume of TB acute-phase patients before and after treatment. In addition, these results were compared with indices from conventional clinical exams to show the coincidence level. Methods: A 3D lung reconstruction method using patient chest radiography was applied to quantify lung-involvement volume using retrospective examinations of 50 patients who were diagnosed with pulmonary TB and treated with two different drugs schemes. Twenty-five patients were treated with Scheme I (rifampicin, isoniazid, and pyrazinamide), whereas twenty-five patients were treated with Scheme II (rifampicin, isoniazid, pyrazinamide, and ethambutol). Acute-phase reaction: Serum exams included C-reactive protein levels, erythrocyte sedimentation rate, and albumin levels. Pulmonary function was tested posttreatment. Results: We found strong agreement between lung involvement and serum indices pre- and posttreatment. Comparison of the functional severity degree with lung involvement based on 3D image quantification for both treatment schemes found a high correlation. Conclusions: The present 3D reconstruction method produced a satisfactory agreement with the acute-phase reaction, most notably a higher significance level with the C-reactive protein. We also found a quite reasonable coincidence between the 3D reconstruction method and the degree of functional lung impairment posttreatment. The performance of the quantification method was satisfactory when comparing the two treatment schemes. Thus, the 3D reconstruction quantification method may be useful tools for monitoring TB treatment. The association with serum indices are not only inexpensive and sensitive but also may be incorporated into the assessment of patients during TB treatment.
DOI: 10.6084/m9.figshare.7743188
title: Heterologous Expression of a Putative ClpC Chaperone Gene Leads to Induction of a Host Metabolite

description: Genome mining provides exciting opportunities for the discovery of natural products. However, in contrast to traditional bioassay-guided approaches, challenges of genome mining include poor or no expression of biosynthetic gene clusters (BGCs). Additionally, given that thousands of BGCs are now available through extensive genome sequencing, how does one select BGCs for discovery? Synthetic biology techniques can be used for BGC refactoring and activation, whereas resistance-gene-directed genome mining is a promising approach to discover bioactive natural products. Here we report the selection of a BGC by applying a resistance-gene-directed approach, cloning of the silent BGC from Micromonospora sp. B006, promoter exchange, and heterologous expression in Streptomyces coelicolor M1152. While we have yet to identify the encoded compound, we unexpectedly observed induction of a host metabolite, which we hypothesize is due to the presence of a caseinolytic protease C (ClpC) chaperone gene in the BGC, suggesting that ClpC chaperones may be used for BGC activation.
DOI: 10.6084/m9.figshare.7743188.v1
title: Heterologous Expression of a Putative ClpC Chaperone Gene Leads to Induction of a Host Metabolite

description: Genome mining provides exciting opportunities for the discovery of natural products. However, in contrast to traditional bioassay-guided approaches, challenges of genome mining include poor or no expression of biosynthetic gene clusters (BGCs). Additionally, given that thousands of BGCs are now available through extensive genome sequencing, how does one select BGCs for discovery? Synthetic biology techniques can be used for BGC refactoring and activation, whereas resistance-gene-directed genome mining is a promising approach to discover bioactive natural products. Here we report the selection of a BGC by applying a resistance-gene-directed approach, cloning of the silent BGC from Micromonospora sp. B006, promoter exchange, and heterologous expression in Streptomyces coelicolor M1152. While we have yet to identify the encoded compound, we unexpectedly observed induction of a host metabolite, which we hypothesize is due to the presence of a caseinolytic protease C (ClpC) chaperone gene in the BGC, suggesting that ClpC chaperones may be used for BGC activation.
DOI: 10.7284/106656
title: Meteorological Station data collected during research cruise AMAT05RR using a SIO MET-System instrument system onboard the platform RV Roger Revelle

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743173
title: Clinicopathological and immunohistochemical analysis of spindle cell squamous cell carcinoma of the tongue: a rare case

description: ABSTRACT Spindle cell squamous cell carcinoma of the tongue is a rare variant of squamous cell carcinoma. This paper reports the case of a spindle cell squamous cell carcinoma of the tongue, in a 64-year-old male patient, and presents a review of the etiopathogenesis, clinicopathological and immunohistochemical features and treatment of the malignancy. The patient presented for evaluation of a painful swelling on his tongue. Extraoral examination revealed palpable submandibular and superior cervical lymph nodes. Based on the presumptive diagnoses of squamous cell carcinoma or malignant salivary gland neoplasm, an incisional biopsy was performed. Histopathological analysis showed a proliferation of atypical spindle cells, exhibiting extensive pleomorphism. Tumor cells were positive for vimentin, P53 and alpha-smooth muscle actin, focally positive for epithelial membrane antigen and P63, and negative for pan-cytokeratin (AE1/AE3), CK7, CD138, CD34, CD56, and S-100. The positivity index for Ki-67 was approximately 40%. The diagnosis of spindle cell squamous cell carcinoma was established and the patient was referred to a head and neck surgery service. In the oral cavity, spindle cell squamous cell carcinoma of the tongue is an aggressive variant of squamous cell carcinoma, which usually presents as an exophytic mass located on the tongue of elderly males. Due to its distinct histopathological characteristics, immunohistochemistry is a valuable and helpful tool to establish the diagnosis of spindle cell squamous cell carcinoma of the tongue.
DOI: 10.6084/m9.figshare.7743179.v1
title: IMPROVEMENT IN OXIDATIVE STRESS AFTER DUODENOJEJUNOSTOMY IN AN EXPERIMENTAL MODEL OF TYPE 2 DIABETES MELLITUS

description: ABSTRACT Background: Type 2 Diabetes Mellitus is a multifactorial syndrome with severe complications. Oxidative stress is accepted as a causal factor of chronic complications Aim: To demonstrate alterations in oxidative stress after metabolic surgery. Methods: Twenty-four 2-day-old Wistar rats were used. In 16, Type 2 Diabetes Mellitus was induced by 100 mg/kg streptozotocin injection. The development of diabetes was confirmed after 10 weeks using an oral glucose tolerance test. Eight diabetic rats composed the diabetic surgical group; the remaining eight composed the diabetic group. Eight animals in which diabetes was not induced formed the clinical control group. The Marchesini technique was used in the diabetic surgical group. After 90 days, the rats were sacrificed, and the oxidative stress markers were measured. Results: Thiobarbituric acid reactive substances, superoxide dismutase and catalase were significantly reduced in the diabetic surgical group compared to the diabetic group. Conclusion: The duodenojejunostomy was effective in controlling the exacerbated oxidative stress present in diabetic rats.