DOI: 10.5061/dryad.30cn0
title: Data from: miR-9a modulates maintenance and ageing of Drosophila germline stem cells by limiting N-cadherin expression

description: Ageing is characterized by a decline in stem cell functionality leading to
 dampened tissue regeneration. While the expression of microRNAs across
 multiple species is markedly altered with age, the mechanism by which they
 govern stem cell-sustained tissue regeneration is unknown. We report that
 in the Drosophila testis, the conserved miR-9a is expressed in germline
 stem cells and its levels are significantly elevated during ageing.
 Transcriptome and functional analyses show that miR-9a directly regulates
 the expression of the adhesion molecule N-cadherin (N-cad). miR-9a null
 mutants maintain a higher number of stem cells even in the aged tissue.
 Remarkably, this rise fails to improve tissue regeneration and results in
 reduced male fertility. Similarly, overexpression of N-cad also results in
 elevated stem cell number and decreased regeneration. We propose that
 miR-9a downregulates N-cad to enable adequate detachment of stem cells
 toward differentiation, thus providing the necessary directionality toward
 terminal differentiation and spermatogenesis.
description: nCounter miRNA assay -
 w1118 1 day Replicate #1NanoString nCounter
 miRNA assay from Drosophila testes, Genotype w1118, Age 1 day, Replicate
 #1w1118_1day_Rep1.RCCnCounter miRNA assay - w1118 1 day Replicate #2NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118, Age 1 day, Replicate #2w1118_1day_Rep2.RCCnCounter miRNA assay - w1118 15 days Replicate #1NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118, Age 15 days, Replicate #1w1118_15day_Rep1.RCCnCounter miRNA assay - w1118 15 days Replicate #2NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118, Age 15 days, Replicate #2w1118_15day_Rep2.RCCnCounter miRNA assay - w1118 30 days Replicate #1NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118, Age 30 days, Replicate #1w1118_30day_Rep1.RCCnCounter miRNA assay - w1118 30 days Replicate #2NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2w1118_30day_Rep2.RCCRNA-Seq - w1118 1day Replicate #1 Lane 1RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 1 day, Replicate #1, Lane 1w1118_1day_Rep1_L1.fastq.gzRNA-Seq - w1118 1day Replicate #1 Lane 2RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 1 day, Replicate #1, Lane 2w1118_1day_Rep1_L2.fastq.gzRNA-Seq - w1118 1day Replicate #2 Lane 1RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 1 day, Replicate #2, Lane 1w1118_1day_Rep2_L1.fastq.gzRNA-Seq - w1118 1day Replicate #2 Lane 2RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 1 day, Replicate #2, Lane 2w1118_1day_Rep2_L2.fastq.gzRNA-Seq - w1118 30 days Replicate #1 Lane 1 Read 1RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #1, Lane 1, Read 1w1118_30day_Rep1_L1_R1.fastq.gzRNA-Seq - w1118 30 days Replicate #1 Lane 1 Read 2RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #1, Lane 1, Read 2w1118_30day_Rep1_L1_R2.fastq.gzRNA-Seq - w1118 30 days Replicate #1 Lane 2 Read 1RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #1, Lane 2, Read 1w1118_30day_Rep1_L2_R1.fastq.gzRNA-Seq - w1118 30 days Replicate #1 Lane 2 Read 2RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #1, Lane 2, Read 2w1118_30day_Rep1_L2_R2.fastq.gzRNA-Seq - w1118 30 days Replicate #2 Lane 1 Read 1RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 1, Read 1w1118_30day_Rep2_L1_R1.fastq.gzRNA-Seq - w1118 30 days Replicate #2 Lane 1 Read 2RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 1, Read 2w1118_30day_Rep2_L1_R2.fastq.gzRNA-Seq - w1118 30 days Replicate #2 Lane 2 Read 1RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 2, Read 1w1118_30day_Rep2_L2_R1.fastq.gzRNA-Seq - w1118 30 days Replicate #2 Lane 2 Read 2RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 2, Read 2w1118_30day_Rep2_L2_R2.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #1 Lane 1 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #1, Lane 1, Read 1mir9a_KO_1day_Rep1_L1_R1.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #1 Lane 1 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #1, Lane 1, Read 2mir9a_KO_1day_Rep1_L1_R2.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #1 Lane 2 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #1, Lane 2, Read 1mir9a_KO_1day_Rep1_L2_R1.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #1 Lane 2 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #1, Lane 2, Read 2mir9a_KO_1day_Rep1_L2_R2.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #2 Lane 1 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #2, Lane 1, Read 1mir9a_KO_1day_Rep2_L1_R1.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #2 Lane 1 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #2, Lane 1, Read 2mir9a_KO_1day_Rep2_L1_R2.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #2 Lane 2 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #2, Lane 2, Read 1mir9a_KO_1day_Rep2_L2_R1.fastq.gzRNA-Seq - mir9a KO 1 day Replicate #2 Lane 2 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #2, Lane 2, Read 2mir9a_KO_1day_Rep2_L2_R2.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #1 Lane 1 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #1, Lane 1, Read 1mir9a_KO_30day_Rep1_L1_R1.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #1 Lane 1 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #1, Lane 1, Read 2mir9a_KO_30day_Rep1_L1_R2.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #1 Lane 2 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #1, Lane 2, Read 1mir9a_KO_30day_Rep1_L2_R1.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #1 Lane 2 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #1, Lane 2, Read 2mir9a_KO_30day_Rep1_L2_R2.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #2 Lane 1 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #2, Lane 1, Read 1mir9a_KO_30day_Rep2_L1_R1.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #2 Lane 1 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #2, Lane 1, Read 2mir9a_KO_30day_Rep2_L1_R2.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #2 Lane 2 Read 1RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #2, Lane 2, Read 1mir9a_KO_30day_Rep2_L2_R1.fastq.gzRNA-Seq - mir9a KO 30 days Replicate #2 Lane 2 Read 2RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #2, Lane 2, Read 2mir9a_KO_30day_Rep2_L2_R2.fastq.gz