DOI: 10.5061/dryad.nq38s
title: Data from: In absence of local adaptation, plasticity and spatially varying selection rule: a view from genomic reaction norms in a panmictic species (Anguilla rostrata)

description: Background: American eel (Anguilla rostrata) is one of the few species for
 which panmixia has been demonstrated at the scale of the entire species.
 As such, the development of long term local adaptation is impossible in
 this species. Instead, both plasticity and spatially varying selection
 have been invoked in explaining how American eel may cope with an unusual
 broad scope of environmental conditions. Here, we address this question
 through transcriptomic analyses and genomic reaction norms of eels from
 two geographic origins reared in controlled environments. Results: The
 null hypothesis of no difference of gene expression between eels from the
 two origins was rejected. Many unique transcripts and two out of seven
 gene clusters showed significant difference in expression, both at time of
 capture and after three months of common rearing. Differences in
 expression were observed at numerous genes representing many functional
 groups when comparing eels from a same origin reared in different salinity
 conditions. Plastic response to different rearing conditions varied among
 gene clusters with three clusters showing significant origin-environment
 interactions translating differential genomic norms of reaction. Most
 genes and functional categories showing differences between origins were
 previously shown to be differentially expressed in a study comparing
 transcription profiles between adult European eels acclimated to different
 salinities. Conclusions: These results emphasize that while plasticity in
 expression may be important, there is also a role for local genetic
 (and/or epigenetic) differences in explaining the extent of differences in
 gene expression between eels from different geographic origins. Such
 differences match those reported in genetically distinct populations in
 other fishes, both in terms of proportion of genes that are differentially
 expressed and the diversity of biological functions being involved. We
 thus propose that genetic differences between glass eels of different
 origins caused by spatially varying selection due to local environmental
 conditions translates into transcriptomic differences (including different
 genomic norms of reaction) which may in turn explain part of the
 phenotypic variance observed between different habitats colonized by eels.
description: Dryad raw data
 microarraysFirsts Sheet -- Column A
 : gene number ; Column B : Mira River/Grande Riviere Blanche fold change
 at the river mouth ; Column C : Mira River/Grande Riviere Blanche fold
 change after 3 months in fresh water ; Column D : Mira River/Grande
 Riviere Blanche fold change after 3 months in brackish water ; Column E :
 Grande Rivière Blanche in brackish water vs fresh water fold change after
 3 months ; Column F : Mira River in brackish water vs fresh water fold
 change after 3 months ; Column G : Gene name and basic annotation. Second
 Sheet -- Column A : Gene name ; Column B : sequence length ; Column C :
 Consensus sequence ; Column D to G : blast result.