cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/27j4v-bfp05
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-35/rawdata/</p>exsD-mini-cell_target_21.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-35/rawdata/exsD-mini-cell_target_21.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_21_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-35/3dimage_105383/exsD-mini-cell_target_21_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_21.mrc.mdoc,  64.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-35/rawdata/exsD-mini-cell_target_21.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.cm54wb
title: Occurrence Download

description: A dataset containing 474 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Trochalopteron formosum",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 474 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045043-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ng6as-p6c20
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-27/rawdata/</p>Ecoli_Conjugation.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-27/rawdata/Ecoli_Conjugation.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_Conjugation_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-27/3dimage_107056/Ecoli_Conjugation_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_Conjugation.mrc.mdoc,  50.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-27/rawdata/Ecoli_Conjugation.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ce6pq-79029
title: MEF cells - Dynamin2 knockdown

description: Dynamin2 knocked down with retroviral delivery of shRNA to study mitochondrial division. grid info - DG2_grid1.
description: <strong>Tilt Series Date:</strong> 2018-11-27</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun Shik from Chan lab helped design shRNA and provided protocols for viral transduction into Chan lab's MEF cells. Shrawan and Danielle made the cell line. Shrawan FIB-milled and performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin (10min)</p>
<strong>Sample Preparation:</strong> Cells were treated with 10 µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-19/rawdata/</p>TS12-pt60_new.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-19/rawdata/TS12-pt60_new.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS11.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-19/rawdata/TS11.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS11_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-19/3dimage_102052/TS11_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1hj1e-8qy65
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-52/rawdata/</p>exsD-mini-cell_target_13.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-52/rawdata/exsD-mini-cell_target_13.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_13_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-52/3dimage_105525/exsD-mini-cell_target_13_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_13.mrc.mdoc,  63.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-52/rawdata/exsD-mini-cell_target_13.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hp1tm-nge36
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-37/rawdata/</p>exsD-mini-cell_target_23.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-37/rawdata/exsD-mini-cell_target_23.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_23_part121_1.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-37/3dimage_105381/exsD-mini-cell_target_23_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_23.mrc.mdoc,  64.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-37/rawdata/exsD-mini-cell_target_23.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/brp2p-vxd29
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-36/rawdata/</p>exsD-mini-cell_target_22.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-36/rawdata/exsD-mini-cell_target_22.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_22_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-36/3dimage_105380/exsD-mini-cell_target_22_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_22.mrc.mdoc,  63.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-36/rawdata/exsD-mini-cell_target_22.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f8s4z-n3191
title: MEF cells - Dynamin2 knockdown

description: Dynamin2 knocked down with retroviral delivery of shRNA to study mitochondrial division. grid info - DG2_grid1.
description: <strong>Tilt Series Date:</strong> 2018-11-27</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun Shik from Chan lab helped design shRNA and provided protocols for viral transduction into Chan lab's MEF cells. Shrawan and Danielle made the cell line. Shrawan FIB-milled and performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin (10min)</p>
<strong>Sample Preparation:</strong> Cells were treated with 10 µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-13/rawdata/</p>TS6.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-13/rawdata/TS6.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS7-pt45.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-13/rawdata/TS7-pt45.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS6_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 784.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-13/3dimage_102046/TS6_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n7b6p-n5d39
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-42/rawdata/</p>exsD-mini-cell_target_31.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-42/rawdata/exsD-mini-cell_target_31.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_31_part121_2.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-42/3dimage_105388/exsD-mini-cell_target_31_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_31.mrc.mdoc,  64.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-42/rawdata/exsD-mini-cell_target_31.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xpcd3-er610
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-29/rawdata/</p>exsD-mini-cell_target_29.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-29/rawdata/exsD-mini-cell_target_29.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_29_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-29/3dimage_105376/exsD-mini-cell_target_29_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_29.mrc.mdoc,  64.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-29/rawdata/exsD-mini-cell_target_29.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fcejv-ysc20
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-63/3dimage_73320/</p>20150511_AK07_10063_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-63/3dimage_73320/20150511_AK07_10063_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10063.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-63/rawdata/20150511_AK07_10063.mrc"
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        20150511_AK07_10063_SIRT_2K.rec~,  5.7 GB  
        
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DOI: 10.15468/dl.79m5rg
title: Occurrence Download

description: A dataset containing 382374 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is MEX.15_1",
 "OccurrenceStatus is Present",
 "TaxonKey is Aves",
 "Year 2020-2025"
 ]
}

 The dataset includes 382374 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045002-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.