DOI: 10.5061/dryad.f1d5h
title: Data from: Pollinator sharing and gene flow among closely related sympatric dioecious fig taxa

description: Hybridization and insect pollination are widely believed to increase rates
 of plant diversification. The extreme diversity of figs (Ficus) and their
 obligate pollinators, fig wasps (Agaonidae), provides an opportunity to
 examine the possible role of pollinator-mediated hybridization in plant
 diversification. Increasing evidence suggests that pollinator sharing and
 hybridization occurs among fig taxa, despite relatively strict
 co-evolution with the pollinating wasp. Using five sympatric dioecious fig
 taxa and their pollinators, we examine the degree of pollinator sharing
 and inter-taxa gene flow. We experimentally test pollinator preference for
 floral volatiles, the main host recognition signal, from different figs.
 All five fig taxa shared pollinators with other taxa and gene flow
 occurred between fig taxa within and between sections. Floral volatiles of
 each taxon attracted more than one pollinator species. Floral volatiles
 were more similar between closely related figs, which experienced higher
 levels of pollinator sharing and inter-taxa gene flow. This study
 demonstrates that pollinator sharing and inter-taxa gene flow occurs among
 closely related sympatric dioecious fig taxa and that pollinators choose
 the floral volatiles of multiple fig taxa. The implications of pollinator
 sharing and inter-taxa gene flow on diversification, occurring even in
 this highly specialized obligate pollination system, requires further
 study.
description: Supplementary Table
 S1_S8 and Figure S1_S3.160216Table S1. Samples
 information of fig and fig wasp for Microsatellite or COI sequences.Table
 S2. Microsatellite primers of Ficus plants used in this paper.Table S3.
 Fig volatile samples of receptive phase in Menglun site.Table S4. Genetic
 distance matrix (Mean± SE) for pollinator clades.Table S5. Frequency of
 associations between sympatric Ficus species and Ceratosolen species
 basing on 87 de novo sequenced Ceratosolen wasps.Table S6. Posterior
 probabilities of assignment of 13 individuals identiﬁed as putative
 hybrids from Bayesian clustering analysis (K = 5) using a priori species
 identity information with migration rate priors of 0.10, 0.05, 0.01 are
 shown.Table S7. Pairwise migration rates (MR) for five sympatric
 morphological figs estimated in BayesAss.Table S8. Tentatative results of
 occurrences (Occ) and relative abundances (Mean, SD) of the compounds
 found in fig volatiles at receptive phase of five morphological fig
 species.Figure S1. Bayesian tree of all Ceratosolen fig wasps with COI
 gene recored in GenBank.Figure S2 Bayesian tree of Ficus from Subgenus
 Sycomorus based on the ITS-G3pdh combined dataset.Figure S3. Bayesian tree
 of 87 Ceratosolen fig wasps de novo sequenced in this study based on the
 COI gene.Appendix S1. Byesian
 tree of all Ceratosolen species with COI gene recored in
 GenBankAppendix S1. Byesian
 tree of all Ceratosolen species with COI gene recored in
 GenBank.nexusAppendix S2. Bayesian
 tree of sympatric figs species from Subgenus
 Sycomorus.nexusAppendix S2. Bayesian
 tree of sympatric figs species from Subgenus
 Sycomorus.nexusAppendix S3. Byesian
 tree of 87 de nove sequenced Ceratosolen fig waspsAppendix S3. Byesian
 tree of 87 de nove sequenced Ceratosolen fig
 wasps.nexusAppendix S4. Ficus SSR
 data 11loci_278indAppendix S4. Ficus SSR
 data 11loci_278ind.txtAppendix S5. List of fig
 wasp and fig samples used in phylogeny establishmentAppendix S5. List of fig
 wasp and fig samples used in phylogeny
 establishment.xlsx