cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.7zs8ts
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((147.736186 -35.897383, 147.736186 -35.904121, 147.738432 -35.904121, 147.738432 -35.897383, 147.736186 -35.897383))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053865-240626123714530/datasets/export for details.
DOI: 10.5281/zenodo.10392172
title: CLDF dataset derived from Luangthongkum's "Proto-Karen Phonology and Lexicon" from 2019

description: Cite the source of the dataset as:




Luangthongkum, T. (2019). A View on Proto-Karen Phonology and Lexicon, Journal of Southest Asian Linguistics Society, 12.1, i-lii. doi: http://hdl.handle.net/10524/52441
DOI: 10.5281/zenodo.13254728
title: CLDF dataset derived from Luangthongkum's "Proto-Karen Phonology and Lexicon" from 2019

description: Cite the source of the dataset as:




Luangthongkum, Theraphan (2019). A View on Proto-Karen Phonology and Lexicon, Journal of Southest Asian Linguistics Society, 12.1, i-lii. doi: http://hdl.handle.net/10524/52441
DOI: 10.5281/zenodo.6538459
title: CLDF dataset derived from Luangthongkum's "Proto-Karen Phonology and Lexicon" from 2019

description: Cite the source of the dataset as:




Luangthongkum, Theraphan (2019). A View on Proto-Karen Phonology and Lexicon, Journal of Southest Asian Linguistics Society, 12.1, i-lii. doi: http://hdl.handle.net/10524/52441
DOI: 10.5281/zenodo.6538460
title: CLDF dataset derived from Luangthongkum's "Proto-Karen Phonology and Lexicon" from 2019

description: Cite the source of the dataset as: Luangthongkum, T. (2019). A View on Proto-Karen Phonology and Lexicon, Journal of Southest Asian Linguistics Society, 12.1, i-lii. doi: http://hdl.handle.net/10524/52441
DOI: 10.5281/zenodo.5126858
title: CLDF dataset derived from Hóu's "Phonological Database of Chinese Dialects" from 2004

description: Cite the source of the dataset as: Hóu, J. (2004): Xiàndài Hànyǔ fāngyán yīnkù 现代汉语方言音库 [Phonological database of Chinese dialects]. Shànghǎi: Shànghǎi Jiàoyù.
DOI: 10.5281/zenodo.13254732
title: CLDF dataset derived from Hóu's "Phonological Database of Chinese Dialects" from 2004

description: Cite the source of the dataset as:




Hóu, J. (2004): Xiàndài Hànyǔ fāngyán yīnkù 现代汉语方言音库 [Phonological database of Chinese dialects]. Shànghǎi: Shànghǎi Jiàoyù.
DOI: 10.5281/zenodo.5126857
title: CLDF dataset derived from Hóu's "Phonological Database of Chinese Dialects" from 2004

description: Cite the source of the dataset as:




Hóu, J. (2004): Xiàndài Hànyǔ fāngyán yīnkù 现代汉语方言音库 [Phonological database of Chinese dialects]. Shànghǎi: Shànghǎi Jiàoyù.
DOI: 10.5281/zenodo.10907379
title: Ende (Papua New Guinea) IDS wordlist by Kate Lynn Lindsey

description: Cite the source of the dataset as:




Kate Lynn Lindsey. 2020. Ende (Papua New Guinea) dictionary. In: Key, Mary Ritchie & Comrie, Bernard (eds.) The Intercontinental Dictionary Series. Leipzig: Max Planck Institute for Evolutionary Anthropology. (Available online at https://ids.clld.org/)
DOI: 10.5281/zenodo.4280603
title: Ende (Papua New Guinea) IDS wordlist by Kate Lynn Lindsey and Bernard Comrie

description: Cite the source of the dataset as: Kate Lynn Lindsey and Bernard Comrie. 2020. Ende (Papua New Guinea) dictionary. In: Key, Mary Ritchie & Comrie, Bernard (eds.) The Intercontinental Dictionary Series. Leipzig: Max Planck Institute for Evolutionary Anthropology. (Available online at http://ids.clld.org/)
DOI: 10.5281/zenodo.5127829
title: Ende (Papua New Guinea) IDS wordlist by Kate Lynn Lindsey and Bernard Comrie

description: Cite the source of the dataset as: Kate Lynn Lindsey and Bernard Comrie. 2020. Ende (Papua New Guinea) dictionary. In: Key, Mary Ritchie & Comrie, Bernard (eds.) The Intercontinental Dictionary Series. Leipzig: Max Planck Institute for Evolutionary Anthropology. (Available online at http://ids.clld.org/)
DOI: 10.5281/zenodo.13254735
title: CLDF dataset derived from Gao's "Tibeto-Burman languages in China" from 2020

description: Cite the source of the dataset as:




Gao, Tianjun (2020): Reconstruction and analysis of phylogenetic network on Tibeto-Burman languages in China. Journal of Chinese Linguistics, 48:1, 257-293.
DOI: 10.5281/zenodo.5121775
title: CLDF dataset derived from Gao's "Tibeto-Burman languages in China" from 2020

description: Cite the source of the dataset as:




Gao, Tianjun (2020): Reconstruction and analysis of phylogenetic network on Tibeto-Burman languages in China. Journal of Chinese Linguistics, 48:1, 257-293.
DOI: 10.5281/zenodo.5121776
title: CLDF dataset derived from Gao's "Tibeto-Burman languages in China" from 2020

description: Cite the source of the dataset as: Gao, Tianjun (2020): Reconstruction and analysis of phylogenetic network on Tibeto-Burman languages in China. Journal of Chinese Linguistics, 48:1, 257-293.
DOI: 10.6084/m9.figshare.26509591
title: CASIA-FaceV5.zip

description: This is the compressed file of CASIA-FaceV5.
DOI: 10.5281/zenodo.13254739
title: Ende (Papua New Guinea) IDS wordlist by Kate Lynn Lindsey

description: Cite the source of the dataset as:




Kate Lynn Lindsey. 2020. Ende (Papua New Guinea) dictionary. In: Key, Mary Ritchie & Comrie, Bernard (eds.) The Intercontinental Dictionary Series. Leipzig: Max Planck Institute for Evolutionary Anthropology. (Available online at https://ids.clld.org/)
DOI: 10.5281/zenodo.4280602
title: Ende (Papua New Guinea) IDS wordlist by Kate Lynn Lindsey

description: Cite the source of the dataset as:




Kate Lynn Lindsey. 2020. Ende (Papua New Guinea) dictionary. In: Key, Mary Ritchie & Comrie, Bernard (eds.) The Intercontinental Dictionary Series. Leipzig: Max Planck Institute for Evolutionary Anthropology. (Available online at https://ids.clld.org/)
DOI: 10.15468/dl.3676w2
title: Occurrence Download

description: A dataset containing 1308 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Anastrepha fratercula (Wiedemann, 1830)"
 ]
}

 The dataset includes 1308 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053866-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.zwtw7h
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.164515 -35.897383, 149.164515 -35.908612, 149.177989 -35.908612, 149.177989 -35.904121, 149.171252 -35.904121, 149.171252 -35.897383, 149.164515 -35.897383))"
 ]
}

 The dataset includes 5 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053867-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0000595
title: 13C nuclear magnetic resonance spectroscopy (13C NMR) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for 13C nuclear magnetic resonance spectroscopy (13C NMR)
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0001146
title: 1H--13C heteronuclear single quantum coherence (1H-13C HSQC) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for 1H--13C heteronuclear single quantum coherence (1H-13C HSQC)
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0001148
title: 1H--13C heteronuclear multiple bond coherence (13C-1H HMBC) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for 1H--13C heteronuclear multiple bond coherence (13C-1H HMBC)
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0000596
title: distortionless enhancement with polarization transfer (DEPT) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for distortionless enhancement with polarization transfer (DEPT)
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0000593
title: 1H nuclear magnetic resonance spectroscopy (1H NMR) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for 1H nuclear magnetic resonance spectroscopy (1H NMR)
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0000599
title: correlation spectroscopy (COSY) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for correlation spectroscopy (COSY)
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n.1
title: 2-butanone
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: This is a physical chemical entity[CHEBI_24431] associated with a molecule[CHEBI_25367].
 The molecule[CHEBI_25367] can be described by the following structural desciptors[cheminf_000085]:
 InChI descriptor[cheminf_000113]: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3, and canonical SMILES descriptor[cheminf_000007]: CCC(=O)C, and by the IUPAC name[cheminf_000107]: butan-2-one.
 
 The physical chemical entity[CHEBI_24431] has a component solvent[CHEBI_46787] which is described by the canonical SMILES descriptor[cheminf_000007]:
 
 The physical chemical entity[CHEBI_24431] has the following Sample ID as registered in the research data repository chemotion (www.chemotion-repository.net, https://doi.org/10.25504/FAIRsharing.iagXcR): CRS-43170

 The physical chemical entity[CHEBI_24431] can be described by the physical descriptors [CHEMINF_000025]:
 Melting point descriptor[CHEMINF_000256]: 
 Boiling point descriptor[CHEMINF_000257]: 
 Refractive index descriptor[CHEMINF_000253]:

 The physical chemical entity[CHEBI_24431] can be further described by the following assays[OBI:0000070][CHMO:0001133]:
 
 CHMO:0000596 | distortionless enhancement with polarization transfer (DEPT)
 
 CHMO:0000595 | 13C nuclear magnetic resonance spectroscopy (13C NMR)
 
 CHMO:0000593 | 1H nuclear magnetic resonance spectroscopy (1H NMR)
 
 CHMO:0000599 | correlation spectroscopy (COSY)
 
 CHMO:0001146 | 1H--13C heteronuclear single quantum coherence (1H-13C HSQC)
 
 CHMO:0000596 | distortionless enhancement with polarization transfer (DEPT)
 
 CHMO:0001148 | 1H--13C heteronuclear multiple bond coherence (13C-1H HMBC)
 
 The physical chemical entity[CHEBI_24431] was deposited to the Molecule Archive of the Karlsruhe Insitute of Technology (KIT) with the following Sample ID: 

 Used ontologies:
 CHEBI - Chemical Entities of Biological Interest
 CHEMINF - chemical information ontology (information entities about chemical entities)
 CHMO - Chemical Methods Ontology
 OBI - Ontology for Biomedical Investigations
DOI: 10.14272/zwehnkrnpovvgh-uhfffaoysa-n/chmo0000596.1
title: distortionless enhancement with polarization transfer (DEPT) (butan-2-one)
title: InChI=1S/C4H8O/c1-3-4(2)5/h3H2,1-2H3

description: dataset for distortionless enhancement with polarization transfer (DEPT)
DOI: 10.15468/dl.quvf8y
title: Occurrence Download

description: A dataset listing the 1 species recorded in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-106.09282 31.84028,-105.87901 31.86598,-105.87355 31.93224,-105.86108 31.99319,-105.89156 32.00982,-105.92619 32.0306,-106.12587 31.98717,-106.09282 31.84028))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Solanum jamesii Torr."
 ]
}

 The dataset's 1 records were derived from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053863-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/tcgw7t
title: A checklist of the reptiles and amphibians of Arabuko-Sokoke Forest, Kenya, 2019

description: This Checklist lists the 88 species of reptiles and 30 species of amphibians recorded within the boundary of Arabuko-Sokoke Forest on the north coast of Kenya. Arabuko-Sokoke is internationally known for its rich and threatened biodiversity and, in spite of being a ‘dry’ coastal forest, holds 33 species of frog. While the list represents studies carried out on the herps of the forest over a period of more than 80 years, relatively few experts have spent significant time in the forest and there are still species constantly being discovered in the forest. Broadley's Dwarf Gecko (Lygodactylus broadleyi) was discovered in 2018 and earlier Usambara Forest Gecko Cnemaspis africana together with two undescribed limbless skinks in the genera Scolecoseps and Melanoseps. The checklist uses the accepted scientific names of species as at date of publication including authorship with additional columns as per Darwin Core Standards. The records were collected primarily through sight observations and capture of individuals. The data were digitized by A Rocha Kenya and verified by other lead authors.
DOI: 10.15468/dl.4eagm9
title: Occurrence Download

description: A dataset containing 54 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON ((-180 -90, 180 -90, 180 90, -180 90, -180 -90))",
 "Depth is greater than or equal to 0m",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Material citation, Material sample, Observation, Specimen)"
 ]
 }
 },
 "TaxonKey is Dermochelyidae",
 "CoordinateUncertaintyInMeters is less than 1e+05"
 ]
}

 The dataset includes 54 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053868-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3dwp52
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((147.22639 -35.901875, 147.22639 -35.904121, 147.230882 -35.904121, 147.230882 -35.901875, 147.22639 -35.901875))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053869-240626123714530/datasets/export for details.
DOI: 10.6084/m9.figshare.26509315
title: Source Data Extended Data Figure 6a-S1KO

description: This file contains the source data for ChIP-seq and 4C-seq of the S1KO cells, specifically for Extended Data Figure 6a.<b>a,</b> Screenshot of UCSC tracks (chr5:170,745,519-171,087,194) depicting aligned H3K27ac ChIP-seq and H3K27me3 ChIP-seq signal tracks, 4C-seq loop tracks of promoter-enhancer loops related to FGF18 promoter region in EV, S1KO and DKO cells. The FGF18 promoter is highlighted in orange line. Red arrows indicate the promoter-enhancer loops of DKO with higher H3K27ac signals than EV and S1KO conditions.
DOI: 10.6084/m9.figshare.26509315.v1
title: Source Data Extended Data Figure 6a-S1KO

description: This file contains the source data for ChIP-seq and 4C-seq of the S1KO cells, specifically for Extended Data Figure 6a.<b>a,</b> Screenshot of UCSC tracks (chr5:170,745,519-171,087,194) depicting aligned H3K27ac ChIP-seq and H3K27me3 ChIP-seq signal tracks, 4C-seq loop tracks of promoter-enhancer loops related to FGF18 promoter region in EV, S1KO and DKO cells. The FGF18 promoter is highlighted in orange line. Red arrows indicate the promoter-enhancer loops of DKO with higher H3K27ac signals than EV and S1KO conditions.
DOI: 10.34740/kaggle/dsv/9124371
title: Global Genomic Neurofibromatosis Neoplasia
DOI: 10.15468/dl.f6ftts
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "MULTIPOLYGON (((147.706991 -35.895137, 147.709237 -35.895137, 147.709237 -35.892892, 147.706991 -35.892892, 147.706991 -35.895137)), ((147.706991 -35.895137, 147.704745 -35.895137, 147.704745 -35.901875, 147.700254 -35.901875, 147.700254 -35.908612, 147.702499 -35.908612, 147.702499 -35.904121, 147.713728 -35.904121, 147.713728 -35.897383, 147.706991 -35.897383, 147.706991 -35.895137)))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053870-240626123714530/datasets/export for details.
DOI: 10.6084/m9.figshare.26509324
title: Source Data Extended Data Figure 6a-DKO

description: This file contains the source data for ChIP-seq and 4C-seq of the DKO cells, specifically for Extended Data Figure 6a.<b>a,</b> Screenshot of UCSC tracks (chr5:170,745,519-171,087,194) depicting aligned H3K27ac ChIP-seq and H3K27me3 ChIP-seq signal tracks, 4C-seq loop tracks of promoter-enhancer loops related to FGF18 promoter region in EV, S1KO and DKO cells. The FGF18 promoter is highlighted in orange line. Red arrows indicate the promoter-enhancer loops of DKO with higher H3K27ac signals than EV and S1KO conditions.
DOI: 10.6084/m9.figshare.26509324.v1
title: Source Data Extended Data Figure 6a-DKO

description: This file contains the source data for ChIP-seq and 4C-seq of the DKO cells, specifically for Extended Data Figure 6a.<b>a,</b> Screenshot of UCSC tracks (chr5:170,745,519-171,087,194) depicting aligned H3K27ac ChIP-seq and H3K27me3 ChIP-seq signal tracks, 4C-seq loop tracks of promoter-enhancer loops related to FGF18 promoter region in EV, S1KO and DKO cells. The FGF18 promoter is highlighted in orange line. Red arrows indicate the promoter-enhancer loops of DKO with higher H3K27ac signals than EV and S1KO conditions.
DOI: 10.15468/dl.85erw6
title: Occurrence Download

description: A dataset containing 10721 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Vespertilio murinus Linnaeus, 1758",
 "HasCoordinate is true",
 "Year is greater than or equal to 2011",
 "Year is less than or equal to 2024"
 ]
}

 The dataset includes 10721 records from 176 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053871-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48324/dandi.000713/0.240702.1725
title: Allen Institute - Visual Behavior - Neuropixels

description: Data released here is for archival purposes. The recommended route for interacting with these data is through the AllenSDK (https://github.com/AllenInstitute/AllenSDK/) which provides methods for downloading and processing data. Documentation of the AllenSDK can be found here: https://allensdk.readthedocs.io/en/latest/. Full documentation of the Visual Behavior Neuropixels dataset and tutorials can be found here: https://allensdk.readthedocs.io/en/latest/visual_behavior_neuropixels.html
DOI: 10.15468/dl.vjrp7p
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.26333 -35.901875, 149.26333 -35.904121, 149.270067 -35.904121, 149.270067 -35.908612, 149.272313 -35.908612, 149.272313 -35.901875, 149.26333 -35.901875))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053872-240626123714530/datasets/export for details.
DOI: 10.6084/m9.figshare.26509381
title: Source Data Extended Data Figures 6b and 6c-EV

description: This file contains the source data for ChIP-seq of the EV cells, specifically for Extended Data Figures 6b and 6c.<b>b,</b> Screenshot depicting aligned H3K27ac ChIP-seq, H3K27me3 ChIP-seq, and 4C-seq signal tracks of enhancer 1 (one of the new enhancers found in DKO cells) in EV, S1KO and DKO cells. Enhancer 1 is highlighted in blue. 3C-PCR of enhancer 1 in EV, S1KO and DKO cells by two independent 3C libraries. Data are relative intensity measured with ImageJ. The panel below are the sanger sequencing results for the 3C ligated fragment. <i>FGF18</i> promoter sequence, HindIII cut site and enhancer 1 sequence are indicated. <b>c,</b> Screenshot depicting aligned H3K27ac ChIP-seq, H3K27me3 ChIP-seq and 4C-seq signal tracks of enhancer 2 (another new enhancer found in DKO cells) in EV, S1KO and DKO cells. Enhancer 2 is highlighted in blue.
DOI: 10.6084/m9.figshare.26509381.v1
title: Source Data Extended Data Figures 6b and 6c-EV

description: This file contains the source data for ChIP-seq of the EV cells, specifically for Extended Data Figures 6b and 6c.<b>b,</b> Screenshot depicting aligned H3K27ac ChIP-seq, H3K27me3 ChIP-seq, and 4C-seq signal tracks of enhancer 1 (one of the new enhancers found in DKO cells) in EV, S1KO and DKO cells. Enhancer 1 is highlighted in blue. 3C-PCR of enhancer 1 in EV, S1KO and DKO cells by two independent 3C libraries. Data are relative intensity measured with ImageJ. The panel below are the sanger sequencing results for the 3C ligated fragment. <i>FGF18</i> promoter sequence, HindIII cut site and enhancer 1 sequence are indicated. <b>c,</b> Screenshot depicting aligned H3K27ac ChIP-seq, H3K27me3 ChIP-seq and 4C-seq signal tracks of enhancer 2 (another new enhancer found in DKO cells) in EV, S1KO and DKO cells. Enhancer 2 is highlighted in blue.
DOI: 10.15468/dl.nktpnd
title: Occurrence Download

description: A dataset containing 865 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Geranium psilostemon Ledeb."
 ]
}

 The dataset includes 865 records from 51 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053873-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gmdapq
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((147.736186 -35.908612, 147.736186 -35.910858, 147.738432 -35.910858, 147.738432 -35.908612, 147.736186 -35.908612))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053874-240626123714530/datasets/export for details.
DOI: 10.25345/c5z892s4f
title: MassIVE MSV000095536 - A metabolism-wide CRISPRi library expands the measurable E. coli metabolome - LC-MS2 dataset
title: In this study, we developed a workflow to systematically and selectively induce increases in metabolites by knocking down enzymes with CRISPR interference (CRISPRi). Therefore, we created a sorted CRISPRi library targeting all 1,515 metabolic genes in the most recent genome-scale metabolic model of E. coli (iML1515). In a first step, we screened the metabolome of the CRISPRi library with a fast flow-injection mass spectrometry, which revealed strong and specific accumulation of 36% of the predicted metabolites in the iML1515 model. The accumulating metabolites were unique to certain knockdowns, especially those metabolites associated with the CRISPRi targeted-pathway. Therefore, we followed-up on accumulating metabolites that were directly associated to the CRISPRi target-gene (i.e. all reactants of the respective enzyme) and measured their fragmentation spectra and chromatographic retention times with LC-MS2. This resulted in experimental fragmentation spectra and chromatographic retention times of 111 metabolites.
DOI: 10.15468/dl.wyeb3n
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.256592 -35.908612, 149.256592 -35.910858, 149.26333 -35.910858, 149.26333 -35.908612, 149.256592 -35.908612))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0053875-240626123714530/datasets/export for details.