cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10438124
title: Association of long non-coding RNA MIAT and MALAT1 expression profiles in peripheral blood of coronary artery disease patients with previous cardiac events

description: Abstract Long non-coding RNAs (lncRNAs) are implicated in various cellular and pathological processes. Two lncRNAs, myocardial infarction-associated transcript (MIAT) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), may be involved in the pathogenesis of coronary artery disease (CAD). Here, we aimed to determine the relative circulating levels of MIAT and MALAT1 in 110 stable CAD patients and 117 controls and to correlate their levels with the clinical and laboratory data. Peripheral blood expression levels were quantified by Real-Time qPCR. The median MIAT expression level in CAD patients was significantly 12-fold higher than controls (p&lt;0.001). Otherwise, the median MALAT1 expression level was comparable in patient and control groups. Both lncRNAs showed significantly higher relative expression levels in patients with positive history of previous cardiac ischemic events, and MIAT showed significantly higher expression in diabetic CAD patients. The area under the curve of MIAT (0.888 ± 0.02 with sensitivity 95.5% and specificity 72.7%), was significantly larger than that of MALAT1 (0.601 ± 0.04 with sensitivity 50% and specificity 63.6%) for detecting the presence of significant CAD. The current findings suggest that lncRNA MIAT could have a diagnostic significance in CAD patients. MALAT1 levels, however, are not sufficiently reliable to have much clinical use in our cases.
DOI: 10.6084/m9.figshare.10438133
title: Olive production in Northern Iran: physical and comparative advantages analysis

description: ABSTRACT: Olive is one of the strategic products of Guilan province, northern Iran. Hence, it is very important to determine the optimal production pattern of olive, given the resources scarcity and the assessment of physical and Comparative Advantage (CA). In this study, three physical advantage indices for olive production in Guilan province during 2008-2016 were investigated. Also, the CA, protection and competitiveness indicators of olive production at Guilan province for 2016-17 cultivation years were calculated using the Policy Analysis Matrix (PAM). Results showed that Guilan province had scale advantage in olive production. The highest degree of olive plantation concentration was on 2008 with scale advantage index of 2.49. Also, results of efficiency advantage showed that, except for 2008, olive production had efficiency advantage over all under study years at Guilan province. Results of Domestic Resource Costs (DRC) and Social Cost Benefit ratio (SCB) indicators for both traditional and commercial olive plantations revealed the CA of olive production in Guilan province. Also, the competitiveness indices values (UCd and UCx) for both traditional and commercial olive plantations were less than 1. So, the olive production systems in Guilan province had the ability to compete with domestic and international competitors.
DOI: 10.6084/m9.figshare.10438127.v1
title: Amino Acid Digestibility of Feed Ingredients in Cecectomized Adult Roosters

description: ABSTRACT A digestibility assay was conducted in order to determine the digestibility coefficients (DC) of amino acids of feed ingredients in cecectomized adult roosters. In total 48 cecectomized adult roosters were used to assess the coefficient of digestibility of 7 ingredients: corn, soybean meal, soybean concentrate, corn gluten meal, wheat bran, peanut meal, and feather meal. Each ingredient was replicated six times and a group of 6 rooster was used to measure endogenous amino acid losses. After 48 hours of fasting, the roosters were tube fed 20g of ingredients tested twice during a day. At 12 hour-intervals excreta and endogenous losses were collected and immediately freeze-dried for further chemical analysis. At the end of the assay, excreta were weighed and samples of ingredients, excreta, and endogenous losses were pooled and analysed for dry matter, nitrogen and amino acid content. The coefficients of indispensable amino acids for the most feed ingredients assessed in the current research were similar to published literature like AMINODat®5.0 and the 4th edition of the Brazilian Tables for Poultry and Swine, except feather meal, in which only digestible Trp and Thr content were similar to literature. Particularly, the coefficients for some amino acids like Arg and Gly exhibited large discrepancies from literature in almost all the feed ingredients assessed herein. In general, using cecectomized adult roosters proved to be a reliable technique to assess the digestibility of feed ingredients used in poultry diets.
DOI: 10.6084/m9.figshare.10438133.v1
title: Olive production in Northern Iran: physical and comparative advantages analysis

description: ABSTRACT: Olive is one of the strategic products of Guilan province, northern Iran. Hence, it is very important to determine the optimal production pattern of olive, given the resources scarcity and the assessment of physical and Comparative Advantage (CA). In this study, three physical advantage indices for olive production in Guilan province during 2008-2016 were investigated. Also, the CA, protection and competitiveness indicators of olive production at Guilan province for 2016-17 cultivation years were calculated using the Policy Analysis Matrix (PAM). Results showed that Guilan province had scale advantage in olive production. The highest degree of olive plantation concentration was on 2008 with scale advantage index of 2.49. Also, results of efficiency advantage showed that, except for 2008, olive production had efficiency advantage over all under study years at Guilan province. Results of Domestic Resource Costs (DRC) and Social Cost Benefit ratio (SCB) indicators for both traditional and commercial olive plantations revealed the CA of olive production in Guilan province. Also, the competitiveness indices values (UCd and UCx) for both traditional and commercial olive plantations were less than 1. So, the olive production systems in Guilan province had the ability to compete with domestic and international competitors.
DOI: 10.6084/m9.figshare.10438124.v1
title: Association of long non-coding RNA MIAT and MALAT1 expression profiles in peripheral blood of coronary artery disease patients with previous cardiac events

description: Abstract Long non-coding RNAs (lncRNAs) are implicated in various cellular and pathological processes. Two lncRNAs, myocardial infarction-associated transcript (MIAT) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), may be involved in the pathogenesis of coronary artery disease (CAD). Here, we aimed to determine the relative circulating levels of MIAT and MALAT1 in 110 stable CAD patients and 117 controls and to correlate their levels with the clinical and laboratory data. Peripheral blood expression levels were quantified by Real-Time qPCR. The median MIAT expression level in CAD patients was significantly 12-fold higher than controls (p&lt;0.001). Otherwise, the median MALAT1 expression level was comparable in patient and control groups. Both lncRNAs showed significantly higher relative expression levels in patients with positive history of previous cardiac ischemic events, and MIAT showed significantly higher expression in diabetic CAD patients. The area under the curve of MIAT (0.888 ± 0.02 with sensitivity 95.5% and specificity 72.7%), was significantly larger than that of MALAT1 (0.601 ± 0.04 with sensitivity 50% and specificity 63.6%) for detecting the presence of significant CAD. The current findings suggest that lncRNA MIAT could have a diagnostic significance in CAD patients. MALAT1 levels, however, are not sufficiently reliable to have much clinical use in our cases.
DOI: 10.6084/m9.figshare.10438109
title: Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses

description: Abstract Drought and cold are the primary factors limiting plant growth worldwide. The Ammopiptanthus mongolicus NAC11 (AmNAC11) gene encodes a stress-responsive transcription factor. Expression of the AmNAC11 gene was induced by drought, cold and high salinity. The AmNAC11 protein was localized in the nucleus and plays an important role in tolerance to drought, cold and salt stresses. We also found that differential expression of AmNAC11 was induced in the early stages of seed germination and was related to root growth. When the AmNAC11 gene was introduced into Arabidopsis thaliana by an Agrobacterium-mediated method, the transgenic lines expressing AmNAC11 displayed significantly enhanced tolerance to drought and freezing stresses compared to wild-type Arabidopsis thaliana plants. These results indicated that over-expression of the AmNAC11 gene in Arabidopsis could significantly enhance its tolerance to drought and freezing stresses. Our study provides a promising approach to improve the tolerance of crop cultivars to abiotic stresses through genetic engineering.
DOI: 10.6084/m9.figshare.10438115
title: Inhibitory Effects of Some Flavonoids on Thioredoxin Reductase Purified from Chicken Liver

description: ABSTRACT Thioredoxin reductases (TrxRs) are selenocysteine-containing flavoenzymes that reduce Trxin NADPH-dependent manner. In the view of the direct vital role of TrxR in a wide range of biochemical and physiological processes, methods to inhibit this enzyme are clinically important. TrxR has recently emerged as a new candidate in anticancer drug investigations because of overexpression in tumorous cells. In this study, TrxR from chick liver was purified 94.6-fold with a yield of 4.86% and a specific activity of 0.19 EU/mg. KM and Vmax values of TrxR for DTNB were calculated as 0.9 mM and 0,03 EU/mL, respectively. Then, the effects of the flavonoids hesperidin, naringenin, chlorogenic acid, ferulic acid, naringin, 3,4-dihydoxybenzoic acid, and ellagic acid on the enzyme activity were evaluated under in-vitro conditions. Ellagic acid showed the strongest inhibitory activity on TrxR with a IC50 value of 18 µM, followed by naringenin and chlorogenic acid with IC50 values of 46.7 µM and 75.8 µM, respectively. Our results showed that flavonoids as natural products are potential inhibitors of TrxRs and the flavonoid content in animal diets may alter metabolic pathways by influencing TrxR activity.
DOI: 10.6084/m9.figshare.10438118
title: Effects of The Dietary Supplementation of Layer Diets with Natural and Synthetic Antioxidant Additives on Yolk Lipid Peroxidation and Fatty Acid Composition of Eggs Stored at Different Temperatures and Duration

description: ABSTRACT In this study, the effects of the supplementation of natural and synthetic antioxidant additives in layer diets on egg weight loss, yolk lipid peroxidation (TBARS values) and fatty acid composition of eggs stored at different temperatures and duration were evaluated. In total, 112 48- weeks-old Bovans White layers were randomly allotted to four dietary treatments with four replicates of seven birds each. The treatments consisted of a control diet, containing no additives, and diets with the inclusion of 200 mg synthetic vitamin E/kg, 1000 mg thyme extract/kg and 1000 mg rosemary extract/kg. Dietary treatments did not influence (p&gt;0.05) relative weight loss of eggs stored for 14, 28, and 56 days, except for those from rosemary-fed hens stored at room temperature on d 42, which were significantly lighter than the eggs from vitamin E- and thyme-fed hens (p&lt;0.001). Relative egg weight loss was significantly higher (p&lt;0.001) when stored at room temperature than under refrigeration, independently of storage time. In eggs stored at room temperature, yolk TBARS values were significantly lower (p&lt;0.001) in the eggs of vitamin E-fed hens, whereas no influence (p&gt;0.05) of dietary treatment on yolk TBARS values were detected in refrigerated eggs. The inclusion of the synthetic and both natural antioxidants in layer diets significantly reduced stearic acid (C18: 0) level in the egg yolk. In addition, only natural antioxidants significantly increased yolk levels of palmitoleic acid (C16: 1) and vaccenic acid (C18: 1n7). The results of the present study showed that adequate storage temperature was more effective in improving egg shelf life than feeding layers synthetic or natural antioxidant additives. However, the positive effects of the evaluated natural antioxidants on yolk fatty acid composition suggest their supplementation to layer diets may provide health benefits to the consumer.
DOI: 10.6084/m9.figshare.10438118.v1
title: Effects of The Dietary Supplementation of Layer Diets with Natural and Synthetic Antioxidant Additives on Yolk Lipid Peroxidation and Fatty Acid Composition of Eggs Stored at Different Temperatures and Duration

description: ABSTRACT In this study, the effects of the supplementation of natural and synthetic antioxidant additives in layer diets on egg weight loss, yolk lipid peroxidation (TBARS values) and fatty acid composition of eggs stored at different temperatures and duration were evaluated. In total, 112 48- weeks-old Bovans White layers were randomly allotted to four dietary treatments with four replicates of seven birds each. The treatments consisted of a control diet, containing no additives, and diets with the inclusion of 200 mg synthetic vitamin E/kg, 1000 mg thyme extract/kg and 1000 mg rosemary extract/kg. Dietary treatments did not influence (p&gt;0.05) relative weight loss of eggs stored for 14, 28, and 56 days, except for those from rosemary-fed hens stored at room temperature on d 42, which were significantly lighter than the eggs from vitamin E- and thyme-fed hens (p&lt;0.001). Relative egg weight loss was significantly higher (p&lt;0.001) when stored at room temperature than under refrigeration, independently of storage time. In eggs stored at room temperature, yolk TBARS values were significantly lower (p&lt;0.001) in the eggs of vitamin E-fed hens, whereas no influence (p&gt;0.05) of dietary treatment on yolk TBARS values were detected in refrigerated eggs. The inclusion of the synthetic and both natural antioxidants in layer diets significantly reduced stearic acid (C18: 0) level in the egg yolk. In addition, only natural antioxidants significantly increased yolk levels of palmitoleic acid (C16: 1) and vaccenic acid (C18: 1n7). The results of the present study showed that adequate storage temperature was more effective in improving egg shelf life than feeding layers synthetic or natural antioxidant additives. However, the positive effects of the evaluated natural antioxidants on yolk fatty acid composition suggest their supplementation to layer diets may provide health benefits to the consumer.
DOI: 10.6084/m9.figshare.10438109.v1
title: Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses

description: Abstract Drought and cold are the primary factors limiting plant growth worldwide. The Ammopiptanthus mongolicus NAC11 (AmNAC11) gene encodes a stress-responsive transcription factor. Expression of the AmNAC11 gene was induced by drought, cold and high salinity. The AmNAC11 protein was localized in the nucleus and plays an important role in tolerance to drought, cold and salt stresses. We also found that differential expression of AmNAC11 was induced in the early stages of seed germination and was related to root growth. When the AmNAC11 gene was introduced into Arabidopsis thaliana by an Agrobacterium-mediated method, the transgenic lines expressing AmNAC11 displayed significantly enhanced tolerance to drought and freezing stresses compared to wild-type Arabidopsis thaliana plants. These results indicated that over-expression of the AmNAC11 gene in Arabidopsis could significantly enhance its tolerance to drought and freezing stresses. Our study provides a promising approach to improve the tolerance of crop cultivars to abiotic stresses through genetic engineering.
DOI: 10.6084/m9.figshare.10438115.v1
title: Inhibitory Effects of Some Flavonoids on Thioredoxin Reductase Purified from Chicken Liver

description: ABSTRACT Thioredoxin reductases (TrxRs) are selenocysteine-containing flavoenzymes that reduce Trxin NADPH-dependent manner. In the view of the direct vital role of TrxR in a wide range of biochemical and physiological processes, methods to inhibit this enzyme are clinically important. TrxR has recently emerged as a new candidate in anticancer drug investigations because of overexpression in tumorous cells. In this study, TrxR from chick liver was purified 94.6-fold with a yield of 4.86% and a specific activity of 0.19 EU/mg. KM and Vmax values of TrxR for DTNB were calculated as 0.9 mM and 0,03 EU/mL, respectively. Then, the effects of the flavonoids hesperidin, naringenin, chlorogenic acid, ferulic acid, naringin, 3,4-dihydoxybenzoic acid, and ellagic acid on the enzyme activity were evaluated under in-vitro conditions. Ellagic acid showed the strongest inhibitory activity on TrxR with a IC50 value of 18 µM, followed by naringenin and chlorogenic acid with IC50 values of 46.7 µM and 75.8 µM, respectively. Our results showed that flavonoids as natural products are potential inhibitors of TrxRs and the flavonoid content in animal diets may alter metabolic pathways by influencing TrxR activity.
DOI: 10.6084/m9.figshare.10438103
title: Microstructural Analysis of AgIn5VI8 (VI: S, Se, Te) Ternary Semiconductors by X-Ray Diffraction

description: This work is a study of the microstructural properties of the polycrystalline ternary compounds AgIn5S8, AgIn5Se8, and AgIn5Te8 by X-ray diffraction technique (XRD). The full-width-half-maximum (FWHM) of the XRD profile is measured as function of the diffraction angle and used to estimate the microstructural parameters. In general, a microstructural characterization by XRD is principally performed by Strain/Size analysis based on the modified Scherrer formula, which in turn, allows for mean grain size and average microstrain to be computed. However, when applied to polycrystalline bulk semiconductors, the modified Scherrer formula gives grain sizes of the order of a few hundreds of nanometers, which is not usually observed in bulk materials. Instead, a new theoretical scheme with misfit dislocations and plastic deformations would be used to calculate the grain size into a bulk. Assuming that these dislocations are of elastic origin, we were able to calculate the misfit dislocations density as function of the elastic constants of the materials. With this, the modified Scherrer formula is corrected to explain the additional XRD line broadening. All microstructure parameters of our samples increase as the atomic radius of the VI-element increases, with elastic constants similar to related semiconducting compounds.
DOI: 10.6084/m9.figshare.10438106
title: Evaluation of Comparative Effect of Feed Additive of Allium Sativum and Zingeber Officinale on Bird Growth and Histomorphometric Characteristics of Small Intestine in Broilers

description: ABSTRACT The present study was conducted to evaluate the effects of supplementation of garlic, ginger in the diets of broiler chickens and assessment in terms of feed intake, growth performance and economics of feeding. The results showed that groups supplemented with 0.5% garlic powder and 0.5% ginger powder has shown significant effects on body weight as compared to the control group at day 28. Groups supplemented with 0.5% garlic powder and 0.5% ginger powder show significant increase in body weight than the groups supplemented with 0.25% garlic powder and 0.25% ginger powder. Between different supplemented groups, villus length and width of duodenum and jejunum of birds served with 0.5% garlic powder and 0.5% ginger powder is significantly higher than the villus length and width of birds supplemented with 0.25% garlic and 0.25% ginger powder. Between different supplemented groups, villus length of ileum of the group supplemented with 0.5% garlic powder is significantly (p&lt;0.05) lower than the villus length of the groups supplemented with 0.25% garlic powder and 0.25% ginger powder.
DOI: 10.6084/m9.figshare.10438100
title: Growth and nutrient accumulation in the aerial part of red Pitaya (Hylocereus sp.)

description: Abstract Knowledge on the amounts of nutrients accumulated by pitaya in its different development stages allows defining the periods in which the essential elements are most required, thus contributing to the elaboration of rational fertilization programs for the crop, reducing production costs. This study aimed to evaluate the growth and nutritional requirement of red pitaya, estimating the accumulation of macro and micronutrients by the plants and determining the periods in which the greatest accumulations occur, as well as the relative growth rate and the relative nutrient absorption rate. Pitaya seedlings were planted in pots containing Argissolo Vermelho-Amarelo Eutrófico (Ultisol) and grown in the open air. The experimental design was randomized blocks, with four replicates and seven sampling times: 0, 60, 120, 180, 240, 300 and 360 DAP (days after planting). At each sampling time, length of cladodes, dry matter of the aerial part and nutrient accumulation in the aerial part were evaluated. Red pitaya plants showed a low growth rate until 60 days and, from this period, its growth increased significantly. Nutrient accumulation occurred in the following descending order: K &gt; Ca &gt; N &gt; Mg = P &gt; S &gt; Na element &gt; Zn &gt; Mn &gt; Fe &gt; B &gt; Cu. K and Zn were, respectively, the most exported macro and micronutrient by pitaya fruits.
DOI: 10.6084/m9.figshare.10438106.v1
title: Evaluation of Comparative Effect of Feed Additive of Allium Sativum and Zingeber Officinale on Bird Growth and Histomorphometric Characteristics of Small Intestine in Broilers

description: ABSTRACT The present study was conducted to evaluate the effects of supplementation of garlic, ginger in the diets of broiler chickens and assessment in terms of feed intake, growth performance and economics of feeding. The results showed that groups supplemented with 0.5% garlic powder and 0.5% ginger powder has shown significant effects on body weight as compared to the control group at day 28. Groups supplemented with 0.5% garlic powder and 0.5% ginger powder show significant increase in body weight than the groups supplemented with 0.25% garlic powder and 0.25% ginger powder. Between different supplemented groups, villus length and width of duodenum and jejunum of birds served with 0.5% garlic powder and 0.5% ginger powder is significantly higher than the villus length and width of birds supplemented with 0.25% garlic and 0.25% ginger powder. Between different supplemented groups, villus length of ileum of the group supplemented with 0.5% garlic powder is significantly (p&lt;0.05) lower than the villus length of the groups supplemented with 0.25% garlic powder and 0.25% ginger powder.