DOI: 10.5061/dryad.j6q573n95
title: Denitrification rates in mountain lake sediments

description: During the last decades, atmospheric nitrogen loading in mountain ranges
 of the Northern Hemisphere has increased substantially, resulting in high
 nitrate concentrations in many lakes. Yet, how increased nitrogen has
 affected denitrification, a key process for nitrogen removal, is poorly
 understood. We measured actual and potential (nitrate and carbon amended)
 denitrification rates in sediments of several lake types and habitats in
 the Pyrenees during the ice-free season. Actual denitrification rates
 ranged from 0 to 9 μmol N2O m−2 h−1 (mean,
 1.5 ± 1.6 SD), whereas potential rates were about 10-times higher. The
 highest actual rates occurred in warmer sediments with more nitrate
 available in the overlying water. Consequently, littoral habitats showed,
 on average, 3-fold higher rates than the deep zone. The highest
 denitrification potentials were found in more productive lakes located at
 relatively low altitude and small catchments, with warmer sediments, high
 relative abundance of denitrification nitrite reductase genes, and
 sulphate-rich waters. We conclude that increased nitrogen deposition has
 resulted in elevated denitrification rates, but not sufficiently to
 compensate for the atmospheric nitrogen loading in most of the highly
 oligotrophic lakes. However, there is potential for high rates, especially
 in the more productive lakes and landscape features largely govern this.
description: Dr Carlos Palacin-Lizarbe made the datasets.
 For further details of the methods see the parent
 publication: Palacin-Lizarbe, C., Camarero, L., Hallin, S., Jones, C. M.
 & Catalan, J. Denitrification rates in lake sediments of mountains
 affected by high atmospheric nitrogen deposition. <i>Sci
 Rep</i><b>10</b>, 3003, doi:10.1038/s41598-020-59759-w (2020). Further details on the denitrification rate measurement method are provided in the publication: Palacin-Lizarbe, C., Camarero, L. & Catalan, J. Estimating sediment denitrification rates using cores and N2O microsensors. <i>J Vis Exp</i>, e58553, doi:doi:10.3791/58553 (2018). Further details on the sediment molecular descriptors (DNA, 16S, nirS, nirK, nosZ1, and nosZ2) are provided in the publication: Palacin-Lizarbe, C. <i>et al. </i>The DNRA-denitrification dichotomy differentiates nitrogen transformation pathways in mountain lake benthic habitats. <i>Front Microbiol </i><b>10</b>, 1229, doi:10.3389/fmicb.2019.01229 (2019).
description: DENITRIFICATION RATES IN MOUNTAIN LAKE SEDIMENTS
 Dryad repository -
 https://doi.org/10.5061/dryad.j6q573n95 This document
 describes four datasets (tab-separated text format tables) related to
 measurements of denitrification rates in mountain lake sediments of the
 Pyrenees. Dr Carlos Palacin-Lizarbe made the datasets, anyone that uses
 these data should reference this DRYAD repository and the parent
 publication: Palacin-Lizarbe, C., Camarero, L., Hallin, S., Jones, C. M.
 & Catalan, J. Denitrification rates in lake sediments of mountains
 affected by high atmospheric nitrogen deposition. <i>Sci
 Rep</i><b>10</b>, 3003, doi:10.1038/s41598-020-59759-w (2020). See this publication for further details on the methods used. <strong>Dataset 1</strong>: Sediment_denitrification_rates_DRYAD.txt<br> Dataset containing all measured denitrification rates and incubation conditions. The dataset contains the following variables: id_rate, id_core, yymmdd_lake_sample, denitrification_rate, nitrate_treatment, temperature_insitu, temperature_incubation, nitrate_actual_plus_added, glucose_added, habitat, site. <strong>Dataset 2</strong>: table_background_DRYAD_def.txt<br> Dataset containing all measured denitrification rates and the background descriptors (landscape, water, sediment). This dataset contain missing values. The dataset contains the following variables: id_core, yymmdd_lake_sample, actual_denitrification_rate, nitrate_add_7_denitrification_rate, nitrate_add_14_denitrification_rate, nitrate_add_28_denitrification_rate, high_nitrate_add_denitrification_rate, site, latitude, longitude, altitude, lake_area, catchment_area, renewal_time, habitat, temperature_insitu, nitrate, nitrite, ammonium, sulphate, DOC, water_column_depth, sediment_layer_depth, organic_matter, nitrmicroogen, d15N, carbon, d13C, carbon_nitrogen_ratio, dry_weight_wet_weight_ratio, sediment_density, sediment_grain_size, DNA, X16S, nirS, nirK, nosZ1, nosZ2. <strong>Dataset 3</strong>: table_actual_denitrification_rates_modeled_DRYAD.txt<br> Dataset containing the actual denitrification rates modelled in Palacin-Lizarbe et al. 2020 Sci Rep and the background descriptors (landscape, water, sediment). The dataset contains the following variables: id_rate, id_core, yymmdd_lake_sample, actual_denitrification_rate, site, latitude, longitude, altitude, lake_area, catchment_area, renewal_time, habitat, temperature_insitu, nitrate, nitrite, ammonium, sulphate, DOC, water_column_depth, sediment_layer_depth, organic_matter, nitrogen, d15N, carbon, d13C, carbon_nitrogen_ratio, dry_weight_wet_weight_ratio, sediment_density, sediment_grain_size, DNA, X16S, nirS, nirK, nosZ1, nosZ2. In the dataset the variables are not transformed. In Palacin-Lizarbe et al. 2020 Sci Rep when developing the models of actual denitrification rates, we use the variables scaled; before being scaled some variables were square root (actual_denitrification_rate, DOC, DNA, X16S, nirS, and nirK) or log<sub>10</sub> (lake_area, catchment_area, renewal_time, nitrite, ammonium, sulphate, dry_weight_wet_weight_ratio, nosZ1, and nosZ2) transformed to reduce the influence of extreme values. <strong>Dataset 4</strong>: table_potential_denitrification_rates_modeled_DRYAD.txt<br> Dataset containing the potential (28 microM nitrate added) denitrification rates modelled in Palacin-Lizarbe et al. 2020 Sci Rep and the background descriptors (landscape, water, sediment). The dataset contains the following variables: id_rate, id_core, yymmdd_lake_sample, nitrate_add_28_denitrification_rate, site, latitude, longitude, altitude, lake_area, catchment_area, renewal_time, habitat, temperature_insitu, nitrate, nitrite, ammonium, sulphate, DOC, water_column_depth, sediment_layer_depth, organic_matter, nitrogen, d15N, carbon, d13C, carbon_nitrogen_ratio, dry_weight_wet_weight_ratio, sediment_density, sediment_grain_size, DNA, X16S, nirS, nirK, nosZ1, nosZ2. In the dataset the variables are not transformed. In Palacin-Lizarbe et al. 2020 Sci Rep when developing the models of potential denitrification rates, we use the variables scaled; before being scaled some variables were square root (nitrate_add_28_denitrification_rate, DOC, DNA, X16S, and nirS) or log<sub>10</sub> (lake_area, catchment_area, renewal_time, nitrite, ammonium, sulphate, dry_weight_wet_weight_ratio, nirK, nosZ1, and nosZ2) transformed to reduce the influence of extreme values. Variables contained in the datasets: id_rate: identification code for each denitrification rate measurement is useful as a link between datasets. id_core: identification code for each sediment core is useful as a link between datasets. yymmdd_lake_sample: The datasets are sorted by this variable. Identification code for each sediment core is useful as a link between datasets. Each code corresponds to the date sampled (year, month, and day), and the Lake, and the number of the sediment core. E.g. 130701Llo3 correspond the 3rd core sampled in Llong Lake on 2013, July 1 (13 07 01). The following are the sampled Lakes, with its abbreviation in brackets: Bergus (Be), Bassa de les Granotes (Bgra), Contraix (Co), Gelat de Bergus (GBe), Llebreta (Lle), Llong (Llo), Plan (Pl), Podo (Po), Redon (Re), Redo Aiguestortes (ReAT), and Redon de Vilamos (ReVil). This variable is useful as a link between datasets. denitrification_rate (micromols N2O m-2 h-1): denitrification rate without identifying the nitrate treatment. actual_denitrification_rate (micromols N2O m-2 h-1): denitrification rate measured without any substrate addition. nitrate_add_7_denitrification_rate (micromols N2O m-2 h-1): denitrification rate measured adding 7 microM nitrate and 1.5 g/L of glucose. nitrate_add_14_denitrification_rate (micromols N2O m-2 h-1): denitrification rate measured adding 14 microM nitrate and 1.5 g/L of glucose. nitrate_add_28_denitrification_rate (micromols N2O m-2 h-1): denitrification rate measured adding 28 microM nitrate and 1.5 g/L of glucose. high_nitrate_add_denitrification_rate (micromols N2O m-2 h-1): denitrification rate measured adding a high concentration of nitrate (&gt;300 microM) and 1.5 g/L of glucose. nitrate_treatment: treatment of nitrate addition in the denitrification measurements. Treatments: actual (without any nitrate added), Add_7 (7 microM nitrate added), Add_14 (14 microM nitrate added), Add_28 (28 microM nitrate added), High_add (&gt;300 microM nitrate added). temperature_incubation (Celsius degrees): constant temperature during the incubation of the denitrification measurement. nitrate_actual_plus_added (microM): actual (in situ) plus added nitrate concentration in the water overlying the sediment core. glucose_added (mg * L-1): added when nitrate was also added. site: Lake (code): Bergus (B), Contraix (C), Bassa de les Granotes (G), Gelat de Bergus (GB), Llebreta (Le), Llong (Lo), Plan (P), Podo (Po), Redon (R), Redo Aiguestortes (RA), and Redon de Vilamos (RV). latitude (N). longitude (E). altitude (m a.s.l.). lake_area (ha). catchment_area (ha). renewal_time (months): mean time that water spends in the lake, retention time and residence time are synonyms of renewal time. habitat: sediment type (code): littoral sediments from rocky areas (R), helophyte Carex rostrata belts (C), beds of isoetid (I) and elodeid (E) macrophytes, and non-vegetated deep (D) sediments. temperature_insitu (Celsius degrees): temperature measured in the water overlying the sediment core just after the sediment core was retrieved. nitrate (microM): nitrate concentration measured in the water overlying the sediment core after less than 4 h of the sediment core retrieval and before the denitrification incubations. nitrite (microM): nitrite concentration measured in the water overlying the sediment core after less than 4 h of the sediment core retrieval and before the denitrification incubations. ammonium (microM): ammonium concentration measured in the water overlying the sediment core after less than 4 h of the sediment core retrieval and before the denitrification incubations. sulphate (microM): sulphate concentration measured in the water overlying the sediment core after less than 4 h of the sediment core retrieval and before the denitrification incubations. DOC (mg*L-1): dissolved organic carbon concentration measured in the water overlying the sediment core after less than 4 h of the sediment core retrieval and before the denitrification incubations. water_column_depth (m): depth of the water column in the sediment core sampling point. Coded as 0.5 for littoral habitats. sediment_layer_depth (cm): depth of the sediment layer described by molecular and abiotic features. organic_matter (%): sediment organic matter content determined by Lost on Ignition. nitrogen (%): sediment nitrogen content. d15N (parts per thousand): sediment delta15N. carbon (%): sediment carbon content. d13C (parts per thousand): sediment delta13C. carbon_nitrogen_ratio (a/a): sediment atomic ratio of the two elements. dry_weight_wet_weight_ratio: sediment dry weight to wet weight ratio. sediment_density (g * cm-3). sediment_grain_size (um): sediment, volume diameter of the mean-sized spherical particle (vol_weighted_mean parameter provided by Mastersizer 2000, Malvern Instruments Ltd, UK). DNA (ng * m-2): sediment DNA content, ng of DNA per m2 in the sediment layer (0-0.5 cm). X16S (copies * m-2): 16S rRNA gene copies per m2 in the sediment layer (0-0.5 cm). nirS (copies * m-2) : nirS gene copies per m2 in the sediment layer (0-0.5 cm). nirK (copies * m-2) : nirK gene copies per m2 in the sediment layer (0-0.5 cm). nosZ1 (copies * m-2) : nosZ1 gene copies per m2 in the sediment layer (0-0.5 cm). nosZ2 (copies * m-2) : nosZ2 gene copies per m2 in the sediment layer (0-0.5 cm).