cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25444/nhlbi.14195507
title: PC12 cell PREM membrane images supporting "The structure and spontaneous curvature of clathrin lattices at the plasma membrane"

description: This dataset is part of a collection of platinum replica electron microscopy data (https://doi.org/10.25444/nhlbi.c.5334212) from the manuscript entitled: "The structure and spontaneous curvature of clathrin lattices at the plasma membrane" by Sochacki et al. 2021 (https://doi.org/10.1016/j.devcel.2021.03.017). <br> Figure 1-2 of this manuscript compare the structure of clathrin in eight different cell types: PC12-GR5 (https://doi.org/10.25444/nhlbi.14195507; male rat adrenal gland, pheochromocytoma), 3T3-L1 (https://doi.org/10.25444/nhlbi.14195129; ATCC® CL-173™, male mouse embryo fibroblast), BS-C-1 (https://doi.org/10.25444/nhlbi.14195216; ATCC® CCL-26™, Cercopithecus aethiops, kidney epithelial, sex unknown), HeLa (https://doi.org/10.25444/nhlbi.14195480; ATCC® CCL-2™, female human cervix epithelial, adenocarcinoma), L6 (https://doi.org/10.25444/nhlbi.14195489; ATCC® CRL-1458™, male rat skeletal muscle, myoblast), MCF7 (https://doi.org/10.25444/nhlbi.14195504; ATCC® HTB-22™, female human mammary gland, epithelial, adenocarcinoma), RAW264.7 (https://doi.org/10.25444/nhlbi.14195537; ATCC® TIB-71™, male mouse, Abelson murine leukemia virus-induced tumor, monocyte/macrophage), and U-87 MG https://doi.org/10.25444/nhlbi.14195561; ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma).<br><br>All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). <br><br>PC12-GR5 (male rat adrenal gland, pheochromocytoma) cells were grown in DMEM (high glucose, pyruvate included, Thermo-Fisher, Gibco™, 11995073) with 5% fetal bovine serum, 5% horse serum, and 1:100 dilution of Penicillin-Streptomycin . They were grown on Poly-lysine coated coverslips for one day prior to unroofing. They were rinsed briefly with unroofing buffer (30mM HEPES, 70mM KCl, 5mM MgCl2, 3mM EGTA at pH 7.4) prior to unroofing. They were unroofed by brief sonication pulse in the presence of 0.5% paraformaldehyde or direct syringe flow in the presence of 2% paraformaldehyde. The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde.Cells were kept in glutaraldehyde at 4 degs C until ready for staining and dehydration; less than two days. The cells were stained with 0.1% tannic acid for 20 minutes, rinsed, then stained with 0.1% uranyl acetate for 20 minutes and dehydrated slowly through a series of increasing ethanol concentration to 100% ethanol. The coverslips were then dried with critical point drying and rotary shadowed with platinum and carbon. The replicas were released from the coverslip with hydrofluoric acid and placed onto a TEM grid for imaging. TEM imaging of replicas was performed on a JEOL 1400 equipped with SerialEM freeware for montaging.
DOI: 10.25444/nhlbi.14195507.v1
title: PC12 cell PREM membrane images supporting "The structure and spontaneous curvature of clathrin lattices at the plasma membrane"

description: This dataset is part of a collection of platinum replica electron microscopy data (https://doi.org/10.25444/nhlbi.c.5334212) from the manuscript entitled: "The structure and spontaneous curvature of clathrin lattices at the plasma membrane" by Sochacki et al. 2021 (https://doi.org/10.1016/j.devcel.2021.03.017). <br> Figure 1-2 of this manuscript compare the structure of clathrin in eight different cell types: PC12-GR5 (https://doi.org/10.25444/nhlbi.14195507; male rat adrenal gland, pheochromocytoma), 3T3-L1 (https://doi.org/10.25444/nhlbi.14195129; ATCC® CL-173™, male mouse embryo fibroblast), BS-C-1 (https://doi.org/10.25444/nhlbi.14195216; ATCC® CCL-26™, Cercopithecus aethiops, kidney epithelial, sex unknown), HeLa (https://doi.org/10.25444/nhlbi.14195480; ATCC® CCL-2™, female human cervix epithelial, adenocarcinoma), L6 (https://doi.org/10.25444/nhlbi.14195489; ATCC® CRL-1458™, male rat skeletal muscle, myoblast), MCF7 (https://doi.org/10.25444/nhlbi.14195504; ATCC® HTB-22™, female human mammary gland, epithelial, adenocarcinoma), RAW264.7 (https://doi.org/10.25444/nhlbi.14195537; ATCC® TIB-71™, male mouse, Abelson murine leukemia virus-induced tumor, monocyte/macrophage), and U-87 MG https://doi.org/10.25444/nhlbi.14195561; ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma).<br><br>All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). <br><br>PC12-GR5 (male rat adrenal gland, pheochromocytoma) cells were grown in DMEM (high glucose, pyruvate included, Thermo-Fisher, Gibco™, 11995073) with 5% fetal bovine serum, 5% horse serum, and 1:100 dilution of Penicillin-Streptomycin . They were grown on Poly-lysine coated coverslips for one day prior to unroofing. They were rinsed briefly with unroofing buffer (30mM HEPES, 70mM KCl, 5mM MgCl2, 3mM EGTA at pH 7.4) prior to unroofing. They were unroofed by brief sonication pulse in the presence of 0.5% paraformaldehyde or direct syringe flow in the presence of 2% paraformaldehyde. The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde.Cells were kept in glutaraldehyde at 4 degs C until ready for staining and dehydration; less than two days. The cells were stained with 0.1% tannic acid for 20 minutes, rinsed, then stained with 0.1% uranyl acetate for 20 minutes and dehydrated slowly through a series of increasing ethanol concentration to 100% ethanol. The coverslips were then dried with critical point drying and rotary shadowed with platinum and carbon. The replicas were released from the coverslip with hydrofluoric acid and placed onto a TEM grid for imaging. TEM imaging of replicas was performed on a JEOL 1400 equipped with SerialEM freeware for montaging.
DOI: 10.25444/nhlbi.14195537.v1
title: RAW264.7 cell PREM membrane images supporting "The structure and spontaneous curvature of clathrin lattices at the plasma membrane"

description: This dataset is part of a collection of platinum replica electron microscopy data (https://doi.org/10.25444/nhlbi.c.5334212) from the manuscript entitled: "The structure and spontaneous curvature of clathrin lattices at the plasma membrane" by Sochacki et al. 2021 (https://doi.org/10.1016/j.devcel.2021.03.017). <br>Figure 1-2 of this manuscript compare the structure of clathrin in eight different cell types: PC12-GR5 (https://doi.org/10.25444/nhlbi.14195507; male rat adrenal gland, pheochromocytoma), 3T3-L1 (https://doi.org/10.25444/nhlbi.14195129; ATCC® CL-173™, male mouse embryo fibroblast), BS-C-1 (https://doi.org/10.25444/nhlbi.14195216; ATCC® CCL-26™, Cercopithecus aethiops, kidney epithelial, sex unknown), HeLa (https://doi.org/10.25444/nhlbi.14195480; ATCC® CCL-2™, female human cervix epithelial, adenocarcinoma), L6 (https://doi.org/10.25444/nhlbi.14195489 ; ATCC® CRL-1458™, male rat skeletal muscle, myoblast), MCF7 (https://doi.org/10.25444/nhlbi.14195504; ATCC® HTB-22™, female human mammary gland, epithelial, adenocarcinoma), RAW264.7 (https://doi.org/10.25444/nhlbi.14195537; ATCC® TIB-71™, male mouse, Abelson murine leukemia virus-induced tumor, monocyte/macrophage), and U-87 MG (https://doi.org/10.25444/nhlbi.14195561; ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma). <br>All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). <br><br>RAW264.7 (ATCC® TIB-71™, male mouse, Abelson murine leukemia virus-induced tumor, monocyte/macrophage) cells were grown in DMEM media supplemented with 10% fetal bovine serum. They were grown on fibronectin coated coverslips for one day prior to unroofing. They were rinsed briefly with unroofing buffer (30mM HEPES, 70mM KCl, 5mM MgCl2, 3mM EGTA at pH 7.4) prior to unroofing. They were unroofed by brief sonication pulse in the presence of 0.5% paraformaldehyde or direct syringe flow in the presence of 2% paraformaldehyde. The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde.Cells were kept in glutaraldehyde at 4 degs C until ready for staining and dehydration; less than two days. The cells were stained with 0.1% tannic acid for 20 minutes, rinsed, then stained with 0.1% uranyl acetate for 20 minutes and dehydrated slowly through a series of increasing ethanol concentration to 100% ethanol. The coverslips were then dried with critical point drying and rotary shadowed with platinum and carbon. The replicas were released from the coverslip with hydrofluoric acid and placed onto a TEM grid for imaging. TEM imaging of replicas was performed on a JEOL 1400 equipped with SerialEM freeware for montaging.
DOI: 10.25444/nhlbi.14195561
title: U87-MG cell PREM membrane images supporting "The structure and spontaneous curvature of clathrin lattices at the plasma membrane"

description: This dataset is part of a collection of platinum replica electron microscopy data (https://doi.org/10.25444/nhlbi.c.5334212) from the manuscript entitled: "The structure and spontaneous curvature of clathrin lattices at the plasma membrane" by Sochacki et al. 2021 (https://doi.org/10.1016/j.devcel.2021.03.017). <br> Figure 1-2 of this manuscript compare the structure of clathrin in eight different cell types: PC12-GR5 (https://doi.org/10.25444/nhlbi.14195507; male rat adrenal gland, pheochromocytoma), 3T3-L1 (https://doi.org/10.25444/nhlbi.14195129; ATCC® CL-173™, male mouse embryo fibroblast), BS-C-1 (https://doi.org/10.25444/nhlbi.14195216; ATCC® CCL-26™, Cercopithecus aethiops, kidney epithelial, sex unknown), HeLa (https://doi.org/10.25444/nhlbi.14195480; ATCC® CCL-2™, female human cervix epithelial, adenocarcinoma), L6 (https://doi.org/10.25444/nhlbi.14195489; ATCC® CRL-1458™, male rat skeletal muscle, myoblast), MCF7 (https://doi.org/10.25444/nhlbi.14195504; ATCC® HTB-22™, female human mammary gland, epithelial, adenocarcinoma), RAW264.7 (https://doi.org/10.25444/nhlbi.14195537; ATCC® TIB-71™, male mouse, Abelson murine leukemia virus-induced tumor, monocyte/macrophage), and U-87 MG https://doi.org/10.25444/nhlbi.14195561; ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma).<br><br>All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). <br><br>U-87 MG (ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma) cells were grown in DMEM media supplemented with 10% fetal bovine serum , Glutamax, and sodium pyruvate. They were grown on Poly-lysine coated coverslips for one day prior to unroofing. They were rinsed briefly with unroofing buffer (30mM HEPES, 70mM KCl, 5mM MgCl2, 3mM EGTA at pH 7.4) prior to unroofing. They were unroofed by brief sonication pulse in the presence of 0.5% paraformaldehyde or direct syringe flow in the presence of 2% paraformaldehyde. The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde.Cells were kept in glutaraldehyde at 4 degs C until ready for staining and dehydration; less than two days. The cells were stained with 0.1% tannic acid for 20 minutes, rinsed, then stained with 0.1% uranyl acetate for 20 minutes and dehydrated slowly through a series of increasing ethanol concentration to 100% ethanol. The coverslips were then dried with critical point drying and rotary shadowed with platinum and carbon. The replicas were released from the coverslip with hydrofluoric acid and placed onto a TEM grid for imaging. TEM imaging of replicas was performed on a JEOL 1400 equipped with SerialEM freeware for montaging.
DOI: 10.25444/nhlbi.14195561.v1
title: U87-MG cell PREM membrane images supporting "The structure and spontaneous curvature of clathrin lattices at the plasma membrane"

description: This dataset is part of a collection of platinum replica electron microscopy data (https://doi.org/10.25444/nhlbi.c.5334212) from the manuscript entitled: "The structure and spontaneous curvature of clathrin lattices at the plasma membrane" by Sochacki et al. 2021 (https://doi.org/10.1016/j.devcel.2021.03.017). <br> Figure 1-2 of this manuscript compare the structure of clathrin in eight different cell types: PC12-GR5 (https://doi.org/10.25444/nhlbi.14195507; male rat adrenal gland, pheochromocytoma), 3T3-L1 (https://doi.org/10.25444/nhlbi.14195129; ATCC® CL-173™, male mouse embryo fibroblast), BS-C-1 (https://doi.org/10.25444/nhlbi.14195216; ATCC® CCL-26™, Cercopithecus aethiops, kidney epithelial, sex unknown), HeLa (https://doi.org/10.25444/nhlbi.14195480; ATCC® CCL-2™, female human cervix epithelial, adenocarcinoma), L6 (https://doi.org/10.25444/nhlbi.14195489; ATCC® CRL-1458™, male rat skeletal muscle, myoblast), MCF7 (https://doi.org/10.25444/nhlbi.14195504; ATCC® HTB-22™, female human mammary gland, epithelial, adenocarcinoma), RAW264.7 (https://doi.org/10.25444/nhlbi.14195537; ATCC® TIB-71™, male mouse, Abelson murine leukemia virus-induced tumor, monocyte/macrophage), and U-87 MG https://doi.org/10.25444/nhlbi.14195561; ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma).<br><br>All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). <br><br>U-87 MG (ATCC® HTB-14™, male human, brain, epithelial, likely glioblastoma) cells were grown in DMEM media supplemented with 10% fetal bovine serum , Glutamax, and sodium pyruvate. They were grown on Poly-lysine coated coverslips for one day prior to unroofing. They were rinsed briefly with unroofing buffer (30mM HEPES, 70mM KCl, 5mM MgCl2, 3mM EGTA at pH 7.4) prior to unroofing. They were unroofed by brief sonication pulse in the presence of 0.5% paraformaldehyde or direct syringe flow in the presence of 2% paraformaldehyde. The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde.Cells were kept in glutaraldehyde at 4 degs C until ready for staining and dehydration; less than two days. The cells were stained with 0.1% tannic acid for 20 minutes, rinsed, then stained with 0.1% uranyl acetate for 20 minutes and dehydrated slowly through a series of increasing ethanol concentration to 100% ethanol. The coverslips were then dried with critical point drying and rotary shadowed with platinum and carbon. The replicas were released from the coverslip with hydrofluoric acid and placed onto a TEM grid for imaging. TEM imaging of replicas was performed on a JEOL 1400 equipped with SerialEM freeware for montaging.
DOI: 10.25444/nhlbi.14204330.v1
title: HeLa (Cilengitide treatment or MBCD treatment) PREM membrane images supporting "The structure and spontaneous curvature of clathrin lattices at the plasma membrane"

description: This dataset is part of a collection of platinum replica electron microscopy data ( https://doi.org/10.25444/nhlbi.c.5334212) from the manuscript entitled:<br>"The structure and spontaneous curvature of clathrin lattices at the plasma membrane" by Sochacki et al. 2021 (https://doi.org/10.1016/j.devcel.2021.03.017).<br><br>Figure 4 of this manuscript performs several drug treatments on HeLa cells to understand their impact on clathrin structure. Treatments include DMSO, actin inhibiting drugs (https://doi.org/10.25444/nhlbi.14204363; Latrunculin A, Jasplikinolide, Cytochalasin D), cholesterol removal (MBCD), and selective integrin blocking (cilengitide).<br><br>All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). <br><br>HeLa cells were treated with drugs to remove cholesterol (methyl-β-cyclodextrin) or block the binding of aVB5 integrin (Cilengitide). Removing cholesterol caused an increase in flat clathrin while blocking integrins caused a decrease in flat clathrin. Additionally, MBCD treated cells were incubated 5 minutes after unroofing in minimal media to find if the flat clathrin could spontaneously curve. All of these data can be compared to untreated HeLa cells uploaded into a different dataset.HeLa (ATCC® CCL-2™, female human cervix epithelial, adenocarcinoma) cells were grown in DMEM media supplemented with 10% fetal bovine serum , Glutamax, and sodium pyruvate. They were grown on Poly-lysine coated coverslips for one day prior to unroofing. They were treated with Cilengitide (10 µM, Sigma-Aldrich, SML1594) or methyl-β-cyclodextrin (9.5 µM, Sigma-Aldrich C4555) added to growth media at normal growth conditions for one hour prior to unroofing/fixation.They were rinsed briefly with unroofing buffer (30mM HEPES, 70mM KCl, 5mM MgCl2, 3mM EGTA at pH 7.4) prior to unroofing. They were unroofed by brief sonication pulse in the presence of 0.5% paraformaldehyde or direct syringe flow in the presence of 2% paraformaldehyde. The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde. For the methyl-β-cyclodextrin (MBCD) 5 minute data, the membranes were unroofed with no fixative present. They were then incubated in stabilization buffer containing MBCD for 5 minutes before being fixed with glutaraldehyde. <br>Cells were kept in glutaraldehyde at 4 degs C until ready for staining and dehydration; less than two days. The cells were stained with 0.1% tannic acid for 20 minutes, rinsed, then stained with 0.1% uranyl acetate for 20 minutes and dehydrated slowly through a series of increasing ethanol concentration to 100% ethanol. The coverslips were then dried with critical point drying and rotary shadowed with platinum and carbon. The replicas were released from the coverslip with hydrofluoric acid and placed onto a TEM grid for imaging. TEM imaging of replicas was performed on a JEOL 1400 equipped with SerialEM freeware for montaging.
DOI: 10.25444/nhlbi.14502183
title: TIRF and PREM correlative images of PC12 cells supporting data in figure 1 of "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells"

description: This dataset is part of a collection of imaging data (https://doi.org/10.25444/nhlbi.c.5405490) from the publication entitled: "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells." by Prasai et al. 2021 (https://doi.org/10.1038/s41467-021-24167-9). <br>It contains the raw images used in fig1a. where representative TIRF images for NPY-mNeongreen and Rab27a-mCherry labeled PC12 cells are shown. It contains raw EM and TIRF images used for correlative TIRF/EM in fig. 1c. And, it contains all the underlying raw tirf images used in the correlative analysis shown in fig. 1b. <br>For TIRF correlative experiment, PC12 cells were were maintained in growth media containing DMEM (Life Technologies), 10% fetal bovine serum (Life Technologies 26140-079), and 1% vol/vol penicillin/streptomycin. They were transiently transfected with DCV marker NPY-mNG or NPY-mCherry and GFP, mcherry or mRFP tagged proteins of interests. Cells were rinsed in intracellular buffer and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde. And TIRF imaging performed using an Olympus IX-81 inverted fluorescence microscope.
DOI: 10.25444/nhlbi.14502183.v1
title: TIRF and PREM correlative images of PC12 cells supporting data in figure 1 of "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells"

description: This dataset is part of a collection of imaging data (https://doi.org/10.25444/nhlbi.c.5405490) from the publication entitled: "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells." by Prasai et al. 2021 (https://doi.org/10.1038/s41467-021-24167-9). <br>It contains the raw images used in fig1a. where representative TIRF images for NPY-mNeongreen and Rab27a-mCherry labeled PC12 cells are shown. It contains raw EM and TIRF images used for correlative TIRF/EM in fig. 1c. And, it contains all the underlying raw tirf images used in the correlative analysis shown in fig. 1b. <br>For TIRF correlative experiment, PC12 cells were were maintained in growth media containing DMEM (Life Technologies), 10% fetal bovine serum (Life Technologies 26140-079), and 1% vol/vol penicillin/streptomycin. They were transiently transfected with DCV marker NPY-mNG or NPY-mCherry and GFP, mcherry or mRFP tagged proteins of interests. Cells were rinsed in intracellular buffer and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde. And TIRF imaging performed using an Olympus IX-81 inverted fluorescence microscope.
DOI: 10.25444/nhlbi.14515671
title: TIRF, dSTORM and PREM images supporting data in figure 2 of "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells"

description: This dataset is part of a collection of imaging data (https://doi.org/10.25444/nhlbi.c.5405490) from the publication entitled: "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells." by Prasai et al. 2021 (https://doi.org/10.1038/s41467-021-24167-9). <br>Figure 2 contains the source of the representative correlative superresolution light and electron microscopy (CLEM) images shown in fig.2a-e. And, it contains all the underlying images that were used in generating fluorescence profiles for dGFP-Rab3a, Rab27a, Rabphilin3a, Granuphilin, and Anti-Rim2 in figure 2h.<br>For CLEM, PC12 cells were were maintained in growth media containing DMEM (Life Technologies), 10% fetal bovine serum (Life Technologies 26140-079), and 1% vol/vol penicillin/streptomycin. They were transiently transfected with DCV marker NPY-mNG and dGFP tagged proteins of interests. Cells were unroofed, fixed, labeled with Alexafluor 647 nanobody, and imaged with Nikon NSTORM microscope for dSTORM. Fluorescently imaged samples were prepared for EM that included fixation in 2% glutaraldehyde, staining with tannic acid and uranyl acetate, serial dehydration with ethanol, critical point drying, metal coating, replica lifting, and imaging with JEOL 1400 equipped with SerialEM for montaging. Processed dSTORM and EM images were correlated using an affine spatial transformation with nearest-neighbor interpolation to map the Gaussian centers of gold nanorods visible in both dSTORM and EM images.
DOI: 10.25444/nhlbi.14515671.v1
title: TIRF, dSTORM and PREM images supporting data in figure 2 of "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells"

description: This dataset is part of a collection of imaging data (https://doi.org/10.25444/nhlbi.c.5405490) from the publication entitled: "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells." by Prasai et al. 2021 (https://doi.org/10.1038/s41467-021-24167-9). <br>Figure 2 contains the source of the representative correlative superresolution light and electron microscopy (CLEM) images shown in fig.2a-e. And, it contains all the underlying images that were used in generating fluorescence profiles for dGFP-Rab3a, Rab27a, Rabphilin3a, Granuphilin, and Anti-Rim2 in figure 2h.<br>For CLEM, PC12 cells were were maintained in growth media containing DMEM (Life Technologies), 10% fetal bovine serum (Life Technologies 26140-079), and 1% vol/vol penicillin/streptomycin. They were transiently transfected with DCV marker NPY-mNG and dGFP tagged proteins of interests. Cells were unroofed, fixed, labeled with Alexafluor 647 nanobody, and imaged with Nikon NSTORM microscope for dSTORM. Fluorescently imaged samples were prepared for EM that included fixation in 2% glutaraldehyde, staining with tannic acid and uranyl acetate, serial dehydration with ethanol, critical point drying, metal coating, replica lifting, and imaging with JEOL 1400 equipped with SerialEM for montaging. Processed dSTORM and EM images were correlated using an affine spatial transformation with nearest-neighbor interpolation to map the Gaussian centers of gold nanorods visible in both dSTORM and EM images.