cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20382251
title: A glycoprotein E gene-deleted bovine alphaherpesvirus 1 strain is attenuated and immunogenic for calves with passive immunity upon intranasal immunization

description: ABSTRACT: Vaccination has been used to prevent the losses associated with Bovine alphaherpesvirus 1 (BoHV-1) infection but passively acquired antibodies may compromise vaccine efficacy. Intranasal immunization (IN) of calves with modified live viral BoHV-1 vaccines has proven to overcome the acquired passive antibodies and confer adequate protection. Herein, we evaluated the safety and immunogenicity of a glycoprotein E-deleted Brazilian BoHV-1 strain (BoHV-1gEΔ) for IN immunization of calves. Ten 1-to-2 months-old calves with virus-neutralizing titers (VN) ranging from 2-64 were immunized IN with viable BoHV-1gEΔ (107.1 TCID50) and four remained as unvaccinated controls (VN titers 8-32). After IN immunization, calves presented a transient (2-6 days) mild nasal secretion and shed the vaccine virus in nasal secretions in low titers (&lt;102.6TCID50/mL) for 4-8 days. Interestingly, the vaccinated calves did not show an increase in VN titers after vaccination. Rather, they presented a gradual reduction in serum VN antibodies in the following weeks - similarly to unvaccinated controls. Upon IN challenge with a virulent heterologous BoHV-1 strain at day 55 post-immunization (107.63TCID50), vaccinated calves shed significantly less virus from day 6 post-challenge onwards (p &lt; 0.07) and for a shorter period of time than the controls (p &lt; 0.0024). Importantly, both the duration and intensity of clinical signs were reduced in vaccinated animals. In addition, vaccinated calves showed an abrupt raise in VN titers post-challenge, indicating adequate immunological priming by vaccination. In summary, immunization of calves harboring passive antibodies with BoHV-1gEΔ by the IN route was able to prime the immunity to afford partial virological and clinical protection upon challenge.
DOI: 10.6084/m9.figshare.20382251.v1
title: A glycoprotein E gene-deleted bovine alphaherpesvirus 1 strain is attenuated and immunogenic for calves with passive immunity upon intranasal immunization

description: ABSTRACT: Vaccination has been used to prevent the losses associated with Bovine alphaherpesvirus 1 (BoHV-1) infection but passively acquired antibodies may compromise vaccine efficacy. Intranasal immunization (IN) of calves with modified live viral BoHV-1 vaccines has proven to overcome the acquired passive antibodies and confer adequate protection. Herein, we evaluated the safety and immunogenicity of a glycoprotein E-deleted Brazilian BoHV-1 strain (BoHV-1gEΔ) for IN immunization of calves. Ten 1-to-2 months-old calves with virus-neutralizing titers (VN) ranging from 2-64 were immunized IN with viable BoHV-1gEΔ (107.1 TCID50) and four remained as unvaccinated controls (VN titers 8-32). After IN immunization, calves presented a transient (2-6 days) mild nasal secretion and shed the vaccine virus in nasal secretions in low titers (&lt;102.6TCID50/mL) for 4-8 days. Interestingly, the vaccinated calves did not show an increase in VN titers after vaccination. Rather, they presented a gradual reduction in serum VN antibodies in the following weeks - similarly to unvaccinated controls. Upon IN challenge with a virulent heterologous BoHV-1 strain at day 55 post-immunization (107.63TCID50), vaccinated calves shed significantly less virus from day 6 post-challenge onwards (p &lt; 0.07) and for a shorter period of time than the controls (p &lt; 0.0024). Importantly, both the duration and intensity of clinical signs were reduced in vaccinated animals. In addition, vaccinated calves showed an abrupt raise in VN titers post-challenge, indicating adequate immunological priming by vaccination. In summary, immunization of calves harboring passive antibodies with BoHV-1gEΔ by the IN route was able to prime the immunity to afford partial virological and clinical protection upon challenge.
DOI: 10.25442/hku.20380311.v1
title: Supporting data for "The role of the gut microbiome and bile acid metabolism in chronic liver diseases"

description: The file contains the supporting data for the thesis entitled "The role of the gut microbiome and bile acid metabolism in chronic liver diseases". <br> Description of the dataset We aim to identify potential new biomarkers for differentiation of MAFLD and chronic HBV infection under different disease courses by bile acid profiling and gut microbiota study. <br> Firstly, we addressed the role of human gut virome in fecal microbiota transplantation (FMT) and metabolic dysfunction-associated fatty liver disease (MAFLD) by investigating the metagenome dataset of a human gut microbiota-associated murine (HMA) model of MAFLD. To this end, gut virome data for the subjects in the HMA model was obtained, the dataset include the gut virome data and a supplementary file. <br> Secondly, we aimed to identify the composition of the gut microbe and profile of ciculating bile acids in a chronic HBV patients. Moreover, we tried to disentangle the relationship between the two chronic liver diseases in te perspective of gut microbiota and bile acid metabolism. Therefore, clinical measurements, bile acid profile, gut microbiome data were obtained.
DOI: 10.25442/hku.20380311
title: Supporting data for "The role of the gut microbiome and bile acid metabolism in chronic liver diseases"

description: The file contains the supporting data for the thesis entitled "The role of the gut microbiome and bile acid metabolism in chronic liver diseases". <br> Description of the dataset We aim to identify potential new biomarkers for differentiation of MAFLD and chronic HBV infection under different disease courses by bile acid profiling and gut microbiota study. <br> Firstly, we addressed the role of human gut virome in fecal microbiota transplantation (FMT) and metabolic dysfunction-associated fatty liver disease (MAFLD) by investigating the metagenome dataset of a human gut microbiota-associated murine (HMA) model of MAFLD. To this end, gut virome data for the subjects in the HMA model was obtained, the dataset include the gut virome data and a supplementary file. <br> Secondly, we aimed to identify the composition of the gut microbe and profile of ciculating bile acids in a chronic HBV patients. Moreover, we tried to disentangle the relationship between the two chronic liver diseases in te perspective of gut microbiota and bile acid metabolism. Therefore, clinical measurements, bile acid profile, gut microbiome data were obtained.
DOI: 10.6084/m9.figshare.20382254
title: EFFECT OF THE MAITLAND CONCEPT TECHNIQUES ON LOW BACK PAIN: A SYSTEMATIC REVIEW

description: ABSTRACT Low back pain has a prevalence that reaches up to 70% of the population between 35-55 years of age and is the principal cause of occupational disability. The scientific evidence on the effect of manual therapy on low back pain is conflicting and there are no specific reviews on the Maitland concept of manual therapy. Therefore, the objective of this systematic review is to analyze the effect of the techniques of the Maitland concept of manual therapy in patients with low back pain and/or determine the level of scientific evidence.For this, a search was carried out in the Cinahl, Medline, Web of Science, PubMed and Scopus databases during the months of January and February 2021 and it was updated in August 2021. In the search, 894 records were obtained, of which 15 randomized clinical trials that obtained a minimum of 3 points out of 5 on the Jadad scale were included.The main results in the current scientific literature suggest that there is solid evidence that the manipulations and mobilizations described in the Maitland Concept, applied alone or in combination with other interventions, reduce pain and disability in subjects with low back pain.The effects on musculature are contradictory. Exercise and patient education increase the effect of manual therapy. Future research requires analyzing whether the effects are preserved in the long term and more homogeneous treatment protocols are needed to determine a prescriptive guideline for manual therapy. Level of evidence I; Systematic review.
DOI: 10.6084/m9.figshare.20382254.v1
title: EFFECT OF THE MAITLAND CONCEPT TECHNIQUES ON LOW BACK PAIN: A SYSTEMATIC REVIEW

description: ABSTRACT Low back pain has a prevalence that reaches up to 70% of the population between 35-55 years of age and is the principal cause of occupational disability. The scientific evidence on the effect of manual therapy on low back pain is conflicting and there are no specific reviews on the Maitland concept of manual therapy. Therefore, the objective of this systematic review is to analyze the effect of the techniques of the Maitland concept of manual therapy in patients with low back pain and/or determine the level of scientific evidence.For this, a search was carried out in the Cinahl, Medline, Web of Science, PubMed and Scopus databases during the months of January and February 2021 and it was updated in August 2021. In the search, 894 records were obtained, of which 15 randomized clinical trials that obtained a minimum of 3 points out of 5 on the Jadad scale were included.The main results in the current scientific literature suggest that there is solid evidence that the manipulations and mobilizations described in the Maitland Concept, applied alone or in combination with other interventions, reduce pain and disability in subjects with low back pain.The effects on musculature are contradictory. Exercise and patient education increase the effect of manual therapy. Future research requires analyzing whether the effects are preserved in the long term and more homogeneous treatment protocols are needed to determine a prescriptive guideline for manual therapy. Level of evidence I; Systematic review.
DOI: 10.6084/m9.figshare.20382257.v1
title: Simulating sugarcane yield response to ETc replacements and green cane trash blanket maintenance in Brazil

description: ABSTRACT Determining the proper crop water requirement associated with the optimum amount of green cane trash blanket (GCTB) on the soil is one of the most discussed issues for sugarcane growers. In this context, this research aimed to evaluate the effects of different amounts of GCTB and ETc replacement scenarios on sugarcane yields across key producing regions in Brazil using the agricultural production systems simulator (APSIM). The sugarcane APSIM (APSIM-Sugar) was parameterized and validated for sugarcane in Brazil, compared to both 100% GCTB cover and bare soil, both fully irrigated. After validation through field data, the APSIM-Sugar model was used to simulate 25 different scenarios with varying GCTB amounts and irrigation demands in 12 regions of Brazil for a 30-year period to estimate stalk yield. Overall, modeled and field data agreed very well regarding soil moisture and biometric and physiological variables, achieving strong modeling efficiency. For most producing regions of Brazil, interaction between the factors did not increase stalk yield significantly, and up to 50% of ETc replacement resulted in the highest increases in stalk yield, with the greatest improvement between 0-25%, producing an average increase of 30 Mg ha-1 per year. The recommended amount of GCTB is at least 5.50 Mg ha-1, taking into account soil health and the need for biofuel generation.
DOI: 10.6084/m9.figshare.20382257
title: Simulating sugarcane yield response to ETc replacements and green cane trash blanket maintenance in Brazil

description: ABSTRACT Determining the proper crop water requirement associated with the optimum amount of green cane trash blanket (GCTB) on the soil is one of the most discussed issues for sugarcane growers. In this context, this research aimed to evaluate the effects of different amounts of GCTB and ETc replacement scenarios on sugarcane yields across key producing regions in Brazil using the agricultural production systems simulator (APSIM). The sugarcane APSIM (APSIM-Sugar) was parameterized and validated for sugarcane in Brazil, compared to both 100% GCTB cover and bare soil, both fully irrigated. After validation through field data, the APSIM-Sugar model was used to simulate 25 different scenarios with varying GCTB amounts and irrigation demands in 12 regions of Brazil for a 30-year period to estimate stalk yield. Overall, modeled and field data agreed very well regarding soil moisture and biometric and physiological variables, achieving strong modeling efficiency. For most producing regions of Brazil, interaction between the factors did not increase stalk yield significantly, and up to 50% of ETc replacement resulted in the highest increases in stalk yield, with the greatest improvement between 0-25%, producing an average increase of 30 Mg ha-1 per year. The recommended amount of GCTB is at least 5.50 Mg ha-1, taking into account soil health and the need for biofuel generation.
DOI: 10.6084/m9.figshare.20382269
title: Development of a new instrument to assess the quality of physicians' delivery of bad news

description: Abstract: Introduction: Most instruments to assess physicians’ delivery of bad news have been developed for patients with cancer and then adapted to other contexts. In clinical practice, some news may not be considered bad by the physicians but may have an important negative impact on the patients’ life. Yet, instruments to assess this communication across diverse clinical settings are needed. Objective: To develop, from the patients’ perspective, an instrument to assess how physicians deliver bad news in clinical practice. Method: This study was conducted using an exploratory qualitative approach by means of semi-structured, in-depth interviews with 109 patients from two referral hospitals in Brazil. Content analysis was used to generate categories, from which the initial instrument items were developed. The clarity and relevance of the items were evaluated by a committee of 11 medical professionals and 10 patients. Results: The instrument included items about the physicians’ attitudes, such as attention, respect, and sincerity, as well as items about sharing information using language that patients could understand. The initial instrument had 19 items, answered in a 5-point Likert scale with labeled endpoints. After evaluation by the committee of judges, 2 items were modified, and 3 were excluded. The final instrument thus had 16 items. Conclusion: A new 16-item instrument was developed from the patients’ perspective to assess physicians’ delivery of bad news. After additional validation, this instrument may be useful in real and diverse bad news settings in clinical practice.
DOI: 10.6084/m9.figshare.20382269.v1
title: Development of a new instrument to assess the quality of physicians' delivery of bad news

description: Abstract: Introduction: Most instruments to assess physicians’ delivery of bad news have been developed for patients with cancer and then adapted to other contexts. In clinical practice, some news may not be considered bad by the physicians but may have an important negative impact on the patients’ life. Yet, instruments to assess this communication across diverse clinical settings are needed. Objective: To develop, from the patients’ perspective, an instrument to assess how physicians deliver bad news in clinical practice. Method: This study was conducted using an exploratory qualitative approach by means of semi-structured, in-depth interviews with 109 patients from two referral hospitals in Brazil. Content analysis was used to generate categories, from which the initial instrument items were developed. The clarity and relevance of the items were evaluated by a committee of 11 medical professionals and 10 patients. Results: The instrument included items about the physicians’ attitudes, such as attention, respect, and sincerity, as well as items about sharing information using language that patients could understand. The initial instrument had 19 items, answered in a 5-point Likert scale with labeled endpoints. After evaluation by the committee of judges, 2 items were modified, and 3 were excluded. The final instrument thus had 16 items. Conclusion: A new 16-item instrument was developed from the patients’ perspective to assess physicians’ delivery of bad news. After additional validation, this instrument may be useful in real and diverse bad news settings in clinical practice.
DOI: 10.6084/m9.figshare.20382271
title: DOUBLE RHOTIC CONSONANTS IN INTERVOCALIC CONTEXT: A STUDY ABOUT THE POSSIBILITY OF GEMINATION IN ANCIENT PORTUGUESE PERIOD

description: ABSTRACT This article aims to study the Phonology of the ancient Portuguese from the troubadour period, specifically investigating the rhotic consonants found in 250 poems in medieval Galician-Portuguese. Our goal is to verify whether there were two rhotic phonemes in opposition at that time or, from the phonological point of view, the sound represented by could be interpreted as a geminate variant from the one represented with . The analysis is based on non-linear phonological models. The method is based on the observation of the possibility of variation in the trill spelling, in order to appoint the relations existing between letters and phonemes, and on the study of the behavior of within the syllable and the word. The cases showed evidences that among vowels the double rhotic fills two slots in the syllable internal organization. We acknowledge the existence of only one rhotic phoneme in Archaic Portuguese, the weak-r, which phonetically presents two variants, a simple one, represented by two graphemes (/), and a geminate one, represented by the double rr ().
DOI: 10.6084/m9.figshare.20382271.v1
title: DOUBLE RHOTIC CONSONANTS IN INTERVOCALIC CONTEXT: A STUDY ABOUT THE POSSIBILITY OF GEMINATION IN ANCIENT PORTUGUESE PERIOD

description: ABSTRACT This article aims to study the Phonology of the ancient Portuguese from the troubadour period, specifically investigating the rhotic consonants found in 250 poems in medieval Galician-Portuguese. Our goal is to verify whether there were two rhotic phonemes in opposition at that time or, from the phonological point of view, the sound represented by could be interpreted as a geminate variant from the one represented with . The analysis is based on non-linear phonological models. The method is based on the observation of the possibility of variation in the trill spelling, in order to appoint the relations existing between letters and phonemes, and on the study of the behavior of within the syllable and the word. The cases showed evidences that among vowels the double rhotic fills two slots in the syllable internal organization. We acknowledge the existence of only one rhotic phoneme in Archaic Portuguese, the weak-r, which phonetically presents two variants, a simple one, represented by two graphemes (/), and a geminate one, represented by the double rr ().
DOI: 10.6084/m9.figshare.20382277
title: Impact of Pre-transport Feed Withdrawal on Welfare and Meat Characteristics of European Quails

description: ABSTRACT The objective of this study was evaluate whether pretransport feed withdrawal affects welfare, carcass, and meat characteristics of European quails. A total of 120 European quails were used, with an initial age of 15 days. Bird feed was removed before transport to the slaughterhouse at the following times, which characterised the different treatments: zero hours, three hours, six hours, nine hours and twelve hours. The transport took 54 minutes to cover 27 kilometres to a commercial slaughterhouse. The birds were slaughtered at 41 days of age. During bleeding, blood samples were collected. Blood glucose, total protein, albumin, uric acid, and corticosterone concentrations were measured. The carcasses and meat characteristics were measured. Poultry body weight decreased and blood glucose concentrations increased with the increase in feed withdrawal time. The treatments did not affect carcass weights. Carcass yields after three hours fasting were similar to those in the six hours and nine hours groups, indicating that gastrointestinal tracts were empty after the third hour. Meat quality was negatively affected (pH, lightness, water holding capacity, cooking loss) by the increase in feed withdrawal time; integrated parameters that characterise dark, firm, dry meat. Pre transport feed withdrawal time should be three hours to empty the gastrointestinal tract and minimise losses in meat quality of European quails. It is necessary to adjust feed withdrawal so that it does not exceed this time, since there is no technical justification for supporting it.
DOI: 10.6084/m9.figshare.20382277.v1
title: Impact of Pre-transport Feed Withdrawal on Welfare and Meat Characteristics of European Quails

description: ABSTRACT The objective of this study was evaluate whether pretransport feed withdrawal affects welfare, carcass, and meat characteristics of European quails. A total of 120 European quails were used, with an initial age of 15 days. Bird feed was removed before transport to the slaughterhouse at the following times, which characterised the different treatments: zero hours, three hours, six hours, nine hours and twelve hours. The transport took 54 minutes to cover 27 kilometres to a commercial slaughterhouse. The birds were slaughtered at 41 days of age. During bleeding, blood samples were collected. Blood glucose, total protein, albumin, uric acid, and corticosterone concentrations were measured. The carcasses and meat characteristics were measured. Poultry body weight decreased and blood glucose concentrations increased with the increase in feed withdrawal time. The treatments did not affect carcass weights. Carcass yields after three hours fasting were similar to those in the six hours and nine hours groups, indicating that gastrointestinal tracts were empty after the third hour. Meat quality was negatively affected (pH, lightness, water holding capacity, cooking loss) by the increase in feed withdrawal time; integrated parameters that characterise dark, firm, dry meat. Pre transport feed withdrawal time should be three hours to empty the gastrointestinal tract and minimise losses in meat quality of European quails. It is necessary to adjust feed withdrawal so that it does not exceed this time, since there is no technical justification for supporting it.
DOI: 10.6084/m9.figshare.20382278
title: Contemporary techniques of da Vinci SP radical prostatectomy: multicentric collaboration and expert opinion

description: ABSTRACT Background The da Vinci SP robot consists of an innovative single port trocar that houses a flexible camera and three biarticulated arms, which minimizes the number of incisions to assess the surgical site, allowing a less invasive procedure. However, due to its recent release in the market, the current literature reporting SP-RARP is still restricted to a few centers. In this scenario, after performing a literature search with all available techniques of SP-RARP, our objective is to report a multicentric opinion of referral centers on different techniques to approach SP-RARP. Results The SP literature is provided by only a few centers due to the limited number of this new console in the market. Five different approaches are available: transperitoneal, extraperitoneal, Retzius-Sparing, transperineal and transvesical. None of the current studies describe long-term functional or oncological outcomes. However, all approaches had satisfactory operative performance with minimum complication rates. Conclusions Several techniques of SP-RARP have been reported in the literature. We performed a multicentric collaboration describing and illustrating the most challenging steps of this surgery. We believe that the details provided in this article are useful teaching material for new centers willing to adopt the SP technology.