cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/fh0dr-2xg17
title: NLRP3

description: Lysate from macrophages expressing ASC / Caspase-1-CARD / NLRP3-PYD-NBD
description: <strong>Tilt Series Date:</strong> 2014-03-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Macrophage lysate</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Hao Wu</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-17-5/rawdata/</p>NLRP3_34k0005.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-17-5/rawdata/NLRP3_34k0005.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3en3p-dgn52
title: B subtilis bMD204

description: mreB-40aa-sfGFP  sfGFP-11aa-mbl::spec  ΔponA::cat"dual-tagged" strain, where mreB is C-terminally tagged and includes a 40 aa linker, while mbl is N-terminally tagged and uses an 11 aa linker.
description: <strong>Tilt Series Date:</strong> 2013-11-15</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> bMD204</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Ethan Garner and Mike Dion</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-11-15-11/rawdata/</p>111510_bs2040011.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-11-15-11/rawdata/111510_bs2040011.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c1j5k-g5r41
title: AIM234

description: Lysate from macrophages expressing ASC / Caspase-1-CARD / AIM2-PYD
description: <strong>Tilt Series Date:</strong> 2014-03-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Macrophage lysate</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Hao Wu</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-17-31/rawdata/</p>AIM234k0003.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-17-31/rawdata/AIM234k0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1a561-wqn70
title: Clostridium thermocellum

description: cellulosome
description: <strong>Tilt Series Date:</strong> 2013-07-29</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Clostridium thermocellum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Matson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth on cellulobiose, anaerobic</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-29-19/rawdata/</p>cthermo0019.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-29-19/rawdata/cthermo0019.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t37j2-9tz27
title: drp1

description: purified drp1
description: <strong>Tilt Series Date:</strong> 2013-09-10</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Drp1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> David Chan</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-10-44/rawdata/</p>drp12750038.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-10-44/rawdata/drp12750038.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.z6z6zq
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Brienomyrus longianalis, Brycinus brevis, Brycinus carmesinus, Brycinus derhami, Brycinus grandisquamis, Brycinus leuciscus, Brycinus longipinnis, Brycinus luteus, Brycinus macrolepidotus, Brycinus poptae, Brycinus rhodopleura, Bryconaethiops boulengeri, Bryconaethiops macrops, Bryconaethiops microstoma, Bryconaethiops quinquesquamae, Bryconalestes derhami, Bryconalestes longipinnis, Bryconalestes tholloni, Buccochromis heterotaenia, Buccochromis lepturus, Buccochromis nototaenia, Buccochromis rhoadesii, Buccochromis spectabilis, Butis butis, Caecobarbus geertsii, Caffrogobius agulhensis, Callochromis macrops, Callochromis melanostigma, Callochromis pleurospilus, Callopanchax monroviae, Callopanchax occidentalis, Callopanchax toddi, Campylomormyrus alces, Campylomormyrus bredoi, Campylomormyrus cassaicus, Campylomormyrus christyi, Campylomormyrus curvirostris, Campylomormyrus elephas, Campylomormyrus luapulaensis, Campylomormyrus mirus, Campylomormyrus numenius, Campylomormyrus orycteropus, Campylomormyrus phantasticus, Campylomormyrus rhynchophorus, Campylomormyrus tamandua, Campylomormyrus tshokwe, Caprichromis liemi, Caprichromis orthognathus, Caranx sexfasciatus, Carasobarbus fritschii, Carasobarbus harterti, Carasobarbus moulouyensis, Carassius auratus, Carcharhinus leucas, Cardiopharynx schoutedeni, Carlarius latiscutatus, Carlarius parkii, Champsochromis caeruleus, Champsochromis spilorhynchus, Channa maculata, Channallabes alvarezi, Channallabes apus, Channallabes longicaudatus, Channallabes ogooensis, Channallabes sanghaensis, Channallabes teugelsi, Chanos chanos, Cheilochromis euchilus, Chelaethiops bibie, Chelaethiops congicus, Chelaethiops elongatus, Chelaethiops minutus, Chelaethiops rukwaensis, Chelidonichthys kumu, Chelon auratus, Chelon labrosus, Chelon melinopterus, Planiliza melinopterus, Chelon planiceps, Chelon ramada, Chelon saliens, Chelonodontops patoca, Chetia brevicauda, Chetia brevis, Chetia flaviventris, Chetia gracilis, Chetia mola, Chetia welwitschi, Chilochromis duponti, Chiloglanis angolensis, Chiloglanis anoterus, Chiloglanis asymetricaudalis, Chiloglanis batesii, Chiloglanis benuensis, Chiloglanis bifurcus, Chiloglanis brevibarbis, Chiloglanis camarabounyi, Chiloglanis cameronensis)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((54.0525 -15.9401,54.0428 -16.0473,54.3021 -16.346,54.6845 -16.3729,54.991 -16.0875,54.9903 -15.9401,54.0525 -15.9401))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006175-250827131500795/datasets/export for details.
DOI: 10.22002/b2459-wn242
title: gh2-2_sq2_cell1

description: GH2-2, sq2, IT 1s
description: <strong>Tilt Series Date:</strong> 2018-04-30</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -4.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> 0</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h.</p>
<strong>Sample Preparation:</strong> Backside manual blotting 3s</p>
  wnj2018-04-30-39_slicer19475.jpg: Fiber coming in wnj2018-04-30-39_slicer19476.jpg: Fiber coming in and connecting to the adjacent sheet (green arrow)? wnj2018-04-30-39_slicer19477.jpg: Purple : fiber .   .  Green : machinery ? wnj2018-04-30-39_slicer19479.jpg: Bump on the OM wnj2018-04-30-39_slicer19480.jpg: Bump on the OM wnj2018-04-30-39_slicer19481.jpg: Purple : periplasmic density .   .  Green : cytoplasmic rode under this density wnj2018-04-30-39_slicer19482.jpg: Machinery with fibril? wnj2018-04-30-39_slicer19507.jpg: Purple: Filament/sheet running under the mb always on side where cellulose is. I see this often
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-30-39/rawdata/</p>sq2_cell1.mrc,  Tilt Series (Pixel Size 0.53 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-30-39/rawdata/sq2_cell1.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq2_cell1.rec,  Reconstruction (Pixel Size 1.06 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-30-39/3dimage_92562/sq2_cell1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1d7da-r8v81
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-197/rawdata/</p>Hpylori_DFliF_FISE_201.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-197/rawdata/Hpylori_DFliF_FISE_201.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_201_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-197/3dimage_113855/Hpylori_DFliF_FISE_201_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ntbt2-9fv85
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-85/rawdata/</p>Bsubtilis_293_053_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-85/rawdata/Bsubtilis_293_053_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_053_af_part41_7.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-85/3dimage_111538/Bsubtilis_293_053_af_part41_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c22cw-30e09
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-128/rawdata/</p>Bsubtilis_293_129_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-128/rawdata/Bsubtilis_293_129_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_129_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-128/3dimage_111600/Bsubtilis_293_129_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xx17g-bv471
title: Shewanella MR1_Iron cores_Chemos

description: Raw data files of Shewanella MR1_Iron cores_Chemos
description: <strong>Tilt Series Date:</strong> 2018-08-09</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These are Shewanella oneidensis MR1 prepared by Sahand (USC) by Chemostat method grown for 2 days and chemically fixed. I (Mohammed) imaged these cells on the Polara to see if the have the iron cores in order to send them to Israel (Sharon Wolf) to perform EDX and confirm the components of the iron cores.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Chemostat 2 days growth and chemically fixed. The samples were prepared by Sahand (USC).</p>
<strong>Sample Preparation:</strong> I prepared these samples by blot force 6, blot time 3s and drain time 1. I also used the Bdellovibrio conditions (force -5, time 4 s). 10 nm Gold nanoparticles were added.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-46/rawdata/</p>SheweTilt205.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-46/rawdata/SheweTilt205.mrc"
        > <i class="download icon"></i> Download </a></p>

        SheweTilt205_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-46/3dimage_94109/SheweTilt205_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bgje9-fag49
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-9/rawdata/</p>Bsubtilis_293_047_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-9/rawdata/Bsubtilis_293_047_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_047_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-9/3dimage_111406/Bsubtilis_293_047_af_full.rec"
        > <i class="download icon"></i> Download </a></p>