cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/rdrcp-d4169
title: E.coli "strain 2" P221D

description: E.coli chemotaxis mutant "strain 2" P221D.Chemoreceptor (chromosomal copy of tsr) locked in a CheA inactivating state.
description: <strong>Tilt Series Date:</strong> 2012-03-08</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> John S. PArkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown overnight in TB medium with gentamycin at 30 degrees. Diluted in fresh medium and grown to log phase, then incubated with penicillin for one hour.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: -4 offset, 3.5 s blot time, no drain, 100 percent humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-08-8/rawdata/</p>strain2marcheightgrid2atlas20006.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-08-8/rawdata/strain2marcheightgrid2atlas20006.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain2marcheightgrid2atlas20006_part128_7.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-03-08-8/3dimage_17570/strain2marcheightgrid2atlas20006_part128_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jst4z-7r223
title: Sulfolobus cells synchronized fo

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2014-02-04</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's samples gridbox 8.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 5 sec, sponge for humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-12/rawdata/</p>atlas30006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-12/rawdata/atlas30006.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas30006_part111_64.rec,  Reconstruction (Pixel Size 1.56 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-12/3dimage_38360/atlas30006_part111_64.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8c4a9-h9v73
title: Myxococcus xanthus delta-pilC

description: grid box YWC060
description: <strong>Tilt Series Date:</strong> 2013-12-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-21/rawdata/</p>20131226_Janelia_YWC060_10042.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-21/rawdata/20131226_Janelia_YWC060_10042.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131226_Janelia_YWC060_10042_part131_1.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-26-21/3dimage_37101/20131226_Janelia_YWC060_10042_part131_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16907664
title: Mythic Genesis: A Formalization of Emergent Narrative 2.0

description: *Definitive work is DOI Version v3

Mythic Calculus — is a plain-language framework for designing and judging stories so they reliably produce meaning. The document fixes a small, stable vocabulary (elements, relations, transformations), an event catalog (wound, test, mentor, reveal, reconcile, oath, deceive, sacrifice, return), and simple tagging rubrics (impact 0–5; positive/negative quality). It introduces dual gates for act transitions (you need both enough meaningful beats and the right kind of beat), an objective way to locate the climax, and a short acceptance-test checklist to prevent dangling subplots and post-climax collapse.

What’s inside





Clear definitions and workflows for generation (prompt → outline → beats) and analysis (draft → tags → diagnosis → fixes)




Guidance for character transformations and how they reshape future moves




A tidy notion of “promising next steps” so plots don’t sprawl




Versioned defaults you can tune per genre while keeping results reproducible
DOI: 10.5281/zenodo.16936121
title: Mythic Calculus: A Formalization of Emergent Narrative v1.0

description: This work lays the foundation upon which all subsequent developments will build. Conceptually coherent and implementation-oriented, but still experimental.

Mythic Calculus — is a plain-language framework for designing and judging stories so they reliably produce meaning. The document fixes a small, stable vocabulary (elements, relations, transformations), an event catalog (wound, test, mentor, reveal, reconcile, oath, deceive, sacrifice, return), and simple tagging rubrics (impact 0–5; positive/negative quality). It introduces dual gates for act transitions (you need both enough meaningful beats and the right kind of beat), an objective way to locate the climax, and a short acceptance-test checklist to prevent dangling subplots and post-climax collapse.

What’s inside





Clear definitions and workflows for generation (prompt → outline → beats) and analysis (draft → tags → diagnosis → fixes)




Guidance for character transformations and how they reshape future moves




A tidy notion of “promising next steps” so plots don’t sprawl




Versioned defaults you can tune per genre while keeping results reproducible



Who it’s for: writers, editors, game/narrative designers, educators, researchers.

Ruminator GPT: https://chatgpt.com/g/g-68a2a3e4cc5c819187c31386eee32c96

(GPT-5 Auto Recommended)
DOI: 10.22002/cnrvp-3mm93
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC106
description: <strong>Tilt Series Date:</strong> 2014-07-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-36/rawdata/</p>20140707_YWC106_10038.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-36/rawdata/20140707_YWC106_10038.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140707_YWC106_10038_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-36/3dimage_44443/20140707_YWC106_10038_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bd9ty-2zz91
title: Rhodobacter sphaeroides JPA 1558

description: cryosections of R sphaeroides strain JPA1558 (TlpT-YFP)
description: <strong>Tilt Series Date:</strong> 2013-06-03</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grow  (1 day) in SUX medium + Naldixic acid (6.25 µl of 20mg/ml stock) + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*Spin down 5ml per strain, resuspend pellets (barely visible) in SUX medium + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*grow overnight.</p>
<strong>Sample Preparation:</strong> cryosectioning by MArk LAdinsky</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-21/rawdata/</p>atlas_grid-2_7x7cryosections0006.mrc,  Tilt Series (Pixel Size 0.38 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-21/rawdata/atlas_grid-2_7x7cryosections0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_grid-2_7x7cryosections0006_part67_9.rec,  Reconstruction (Pixel Size 0.76 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-21/3dimage_26934/atlas_grid-2_7x7cryosections0006_part67_9.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.866tg5
title: Occurrence Download

description: A dataset containing 106 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Symphyotrichum novi-belgii",
 "Country is Hungary",
 "HasCoordinate is true"
 ]
}

 The dataset includes 106 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0048448-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/yhz1h-42b06
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-121/rawdata/</p>RCC745_RELtovis_132.mrc,  Tilt Series (Pixel Size 1.26 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-121/rawdata/RCC745_RELtovis_132.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_132.rec,  Reconstruction (Pixel Size 1.26 nm), 290.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-121/3dimage_12583/RCC745_RELtovis_132.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/683kb-xbk74
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-11-16</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-94/rawdata/</p>RCC745_MGtovis_517.mrc,  Tilt Series (Pixel Size 1.26 nm), 738.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-94/rawdata/RCC745_MGtovis_517.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_517.rec,  Reconstruction (Pixel Size 1.26 nm), 390.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-94/3dimage_11953/RCC745_MGtovis_517.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cjca8-c2683
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 10 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-01-14</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-31/rawdata/</p>stiv10h40021.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-31/rawdata/stiv10h40021.mrc"
        > <i class="download icon"></i> Download </a></p>

        stiv10h40021_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-31/3dimage_37726/stiv10h40021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6wvbd-a9j17
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-72/rawdata/</p>20131221_YWC063_20029.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-72/rawdata/20131221_YWC063_20029.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_20029_part126_34.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-72/3dimage_36484/20131221_YWC063_20029_part126_34.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m6pe1-smr70
title: Rhodobacter sphaeroides JPA 1558

description: cryosections of R sphaeroides strain JPA1558 (TlpT-YFP)
description: <strong>Tilt Series Date:</strong> 2013-06-03</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grow  (1 day) in SUX medium + Naldixic acid (6.25 µl of 20mg/ml stock) + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*Spin down 5ml per strain, resuspend pellets (barely visible) in SUX medium + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*grow overnight.</p>
<strong>Sample Preparation:</strong> cryosectioning by MArk LAdinsky</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-8/rawdata/</p>atlas_grid-2_7x7cryosections0065.mrc,  Tilt Series (Pixel Size 0.38 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-8/rawdata/atlas_grid-2_7x7cryosections0065.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_grid-2_7x7cryosections0065_part67_5.rec,  Reconstruction (Pixel Size 0.76 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-06-03-8/3dimage_26917/atlas_grid-2_7x7cryosections0065_part67_5.rec"
        > <i class="download icon"></i> Download </a></p>