cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647907.v1
title: CIL:19121

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing fat tissue (ft) and labeled nuclei (n). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12583, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647908.v1
title: CIL:19122

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the principal salivary gland (psg). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12584, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647905
title: CIL:19119

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the midgut (mg) and associated trachea (arrow). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12581, from the same image group, is an unlabeld version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820-826.
DOI: 10.6084/m9.figshare.647906
title: CIL:19120

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the Malpighian tubule (mt). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12582, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647903
title: CIL:19117

description: Electron micrograph of corn stunt spiroplasma (CSS) in the wall of the filter chamber (part of the alimentary canal) and in the hemolymph (he) of a vector leafhopper D. gelbus. Note the mainly pleiomorphic forms in host cells and the filamentous/spiral forms (asterisks) in the hemolymph. CIL:12579, from the same image group, is identical to this image but contains labels. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional images from this contributor are available in the Library.
DOI: 10.6084/m9.figshare.647904
title: CIL:19118

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the anterior and posterior midgut (amg and pmg). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12580, from the same image group, is an uncropped unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647905.v1
title: CIL:19119

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the midgut (mg) and associated trachea (arrow). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12581, from the same image group, is an unlabeld version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820-826.
DOI: 10.6084/m9.figshare.647902
title: CIL:19116

description: Electron micrograph of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) in sieve elements and other phloem cells of infected maize leaves. CIL:12578, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional images are available by this contributor.
DOI: 10.6084/m9.figshare.647906.v1
title: CIL:19120

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the Malpighian tubule (mt). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12582, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647901
title: CIL:16311

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This jpg image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the unlabeled tif file CIL:12417. MMV virions (v), budding through the inner nuclear membrane (inm), are accumulating between this membrane and the outer nuclear membrane (onm) as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Ch=chloroplast; cw=cell wall; cy=cytoplasm; N=nucleus, va=cell vacuole Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136. .
DOI: 10.6084/m9.figshare.647902.v1
title: CIL:19116

description: Electron micrograph of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) in sieve elements and other phloem cells of infected maize leaves. CIL:12578, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional images are available by this contributor.
DOI: 10.6084/m9.figshare.647903.v1
title: CIL:19117

description: Electron micrograph of corn stunt spiroplasma (CSS) in the wall of the filter chamber (part of the alimentary canal) and in the hemolymph (he) of a vector leafhopper D. gelbus. Note the mainly pleiomorphic forms in host cells and the filamentous/spiral forms (asterisks) in the hemolymph. CIL:12579, from the same image group, is identical to this image but contains labels. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional images from this contributor are available in the Library.
DOI: 10.6084/m9.figshare.647904.v1
title: CIL:19118

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the anterior and posterior midgut (amg and pmg). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12580, from the same image group, is an uncropped unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647900
title: CIL:16310

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This jpg image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the unlabeled tif file CIL:12415. MMV virions (v), budding through the inner nuclear membrane (inm), are accumulating between this membrane and the outer nuclear membrane (onm) as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Ch=chloroplast; cw=cell wall; cy=cytoplasm; N=nucleus, va=cell vacuole Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647901.v1
title: CIL:16311

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This jpg image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the unlabeled tif file CIL:12417. MMV virions (v), budding through the inner nuclear membrane (inm), are accumulating between this membrane and the outer nuclear membrane (onm) as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Ch=chloroplast; cw=cell wall; cy=cytoplasm; N=nucleus, va=cell vacuole Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136. .
DOI: 10.6084/m9.figshare.647899
title: CIL:16309

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This jpg image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the unlabeled tif file CIL:12416. MMV virions (v), budding through the inner nuclear membrane (inm), are accumulating between this membrane and the outer nuclear membrane (onm) as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Ch=chloroplast; cw=cell wall; cy=cytoplasm; N=nucleus, va=cell vacuole Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647900.v1
title: CIL:16310

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This jpg image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the unlabeled tif file CIL:12415. MMV virions (v), budding through the inner nuclear membrane (inm), are accumulating between this membrane and the outer nuclear membrane (onm) as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Ch=chloroplast; cw=cell wall; cy=cytoplasm; N=nucleus, va=cell vacuole Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647897
title: CIL:13376

description: Z-focal series through HeLa cell in mitosis illustrating MCAK localization in Bod1siRNA cells. Cells were transfected with control (CIL: 13377) or Bod1 (this image) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.
DOI: 10.6084/m9.figshare.647898
title: CIL:13377

description: z-focal series through Hela cell in metaphase illustrating MCAK localization in Bod-1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.
DOI: 10.6084/m9.figshare.647898.v1
title: CIL:13377

description: z-focal series through Hela cell in metaphase illustrating MCAK localization in Bod-1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.
DOI: 10.6084/m9.figshare.647895
title: CIL:13372

description: Z focal series of Bod-1 depleted HeLa cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). Bod1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. This image is the Bod-1 depletion experiment for the control CIL: 13374.
DOI: 10.6084/m9.figshare.647896
title: CIL:13374

description: Z-series of fluorescence images of a Hela cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). This image is the control for the Bod-1 depletion experiment illustrated in CIL: 13372. Bod-1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.
DOI: 10.6084/m9.figshare.647897.v1
title: CIL:13376

description: Z-focal series through HeLa cell in mitosis illustrating MCAK localization in Bod1siRNA cells. Cells were transfected with control (CIL: 13377) or Bod1 (this image) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.
DOI: 10.6084/m9.figshare.647899.v1
title: CIL:16309

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This jpg image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the unlabeled tif file CIL:12416. MMV virions (v), budding through the inner nuclear membrane (inm), are accumulating between this membrane and the outer nuclear membrane (onm) as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Ch=chloroplast; cw=cell wall; cy=cytoplasm; N=nucleus, va=cell vacuole Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647894
title: CIL:12591

description: Micrograph of a semi-thick (1-2 µm) section of an infected maize leaf, with silver-enhanced immunogold labeling, that shows dark staining of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates in phloem tissue. The section was counter stained with toluidine blue. CIL:19129, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. dditional related images from this contributor are available in the Library.
DOI: 10.6084/m9.figshare.647895.v1
title: CIL:13372

description: Z focal series of Bod-1 depleted HeLa cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). Bod1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. This image is the Bod-1 depletion experiment for the control CIL: 13374.