cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7186733.v1
title: Alternative Transcription of Peroxisome Proliferator-Activated Receptor Gamma in the Liver Is Associated with Fatness of Chickens

description: ABSTRACT The expression of four transcription variant of peroxisome proliferator-activated receptor gamma gene (PPARG) (XM_015292931.1; XM_015292932.1; XM_015292933.1 and NM_001001460.1) in the liver of broilers was measured and its correlation with abdominal fat weight and relative abdominal fat content was investigated. The study was conducted with 92 slow-growing crossbred chickens (Cobb males x indigenous Green-legged Partridge female chickens) divided into “fat” and “lean” groups, according to their abdominal fat yield. The NM_001001460.1 transcriptwas upregulated with ratio of means 4.26 (p≤0.01) in the “fat” group in relation to the “lean” group. Expression of this transcript was highly correlated with relative abdominal fat content (0.71, p≤0.01) and abdominal fat weight (0.59, p≤0.01). Two SNPs are located in putative transcription factor binding sites. Mutation -991C&gt;A disrupts PPAR while mutation -884C&gt;T disrupts C/EBP putative binding site. The gene expression analysis of PPARg showed that the expression of the transcripts (NM_001001460.1) was more than four times higher in fat than in lean chickens. These results point out that the peroxisome proliferator-activated receptor gamma NM_001001460.1 transcript could be candidate gene for determination of abdominal fat deposition in the chickens.
DOI: 10.6084/m9.figshare.7482779
title: Subsequent Effect of Lysine Regimens on Egg Characteristics of Native Aseel Chicken

description: ABSTRACT This study was planned to evaluate subsequent effects of rearing under various lysine regimens on egg quality traits in Peshawari, Mushki, Mianwali and Lakha varieties of native Aseel. Ninety six pullets and 12 cockerels (24♀ and 3♂) from each variety were randomly chosen, placed in three-tiered cage units of equal space and standard conditions were followed for their maintenance. These birds were reared on three lysine regimens (L1, L2 and L3), wherein L1 containing 1.3% lysine was given in one phase from week 1-6 of age, L2 regimens entailing 1.4 and 1.2% lysine was smeared in two phases from week 1-3 and 4-6 of age, while in L3 regimen, lysine @ 1.5, 1.3 and 1.1% in diet was fed in three phases from week 1-2, 3-4 and 5-6 of age, respectively. Data were analyzed using two-way factorial ANOVA technique in SAS 9.1under randomized complete block design and significant means were compared through Duncan’s Multiple Range Test. Statistical analysis showed that weight of egg and shell, albumen weight, diameter and index, yolk weight and Haugh units (p≤0.05) improved significantly in L3 in Peshawari Aseel depicting the better egg quality traits. It was concluded that L3 regimen has positive impact on egg characteristics of Aseel chicken.
DOI: 10.6084/m9.figshare.7482779.v1
title: Subsequent Effect of Lysine Regimens on Egg Characteristics of Native Aseel Chicken

description: ABSTRACT This study was planned to evaluate subsequent effects of rearing under various lysine regimens on egg quality traits in Peshawari, Mushki, Mianwali and Lakha varieties of native Aseel. Ninety six pullets and 12 cockerels (24♀ and 3♂) from each variety were randomly chosen, placed in three-tiered cage units of equal space and standard conditions were followed for their maintenance. These birds were reared on three lysine regimens (L1, L2 and L3), wherein L1 containing 1.3% lysine was given in one phase from week 1-6 of age, L2 regimens entailing 1.4 and 1.2% lysine was smeared in two phases from week 1-3 and 4-6 of age, while in L3 regimen, lysine @ 1.5, 1.3 and 1.1% in diet was fed in three phases from week 1-2, 3-4 and 5-6 of age, respectively. Data were analyzed using two-way factorial ANOVA technique in SAS 9.1under randomized complete block design and significant means were compared through Duncan’s Multiple Range Test. Statistical analysis showed that weight of egg and shell, albumen weight, diameter and index, yolk weight and Haugh units (p≤0.05) improved significantly in L3 in Peshawari Aseel depicting the better egg quality traits. It was concluded that L3 regimen has positive impact on egg characteristics of Aseel chicken.
DOI: 10.6084/m9.figshare.10296182
title: Effect of Egg Weight on External and Internal Qualities, Physiological and Hatching Success of Japanese Quail Eggs (Coturnix coturnix japonica)

description: ABSTRACT This experiment was conducted to evaluate the effect of egg weight and egg physical characteristics on embryonic development, hatchability, and hatchling weight of Japanese quails (Coturnix coturnix japonica). In total, 689 eggs were classified in two categories: small (&lt;13.5 g) or large (≥13.5 g), and different external eggshell and internal quality traits were measured. On days 6 and 14 of incubation, tissue triiodothyronine (T3) and thyroxine (T4) were extracted from embryos in both egg sizes and analyzed. Relative to internal egg-quality traits, large eggs had significantly higher yolk height, yolk diameter, yolk weight, albumen height, and albumen weight than small eggs (p≤0.01). However, Haugh unit score (p=0.27) was not significantly different between the two egg sizes. Relative to eggshell quality parameters, large eggs had significantly higher total pore count, surface area, eggshell volume, and eggshell weight than small eggs (p≤0.05), with consequent higher hatchability rate and hatchling weight. Pearson’s correlation coefficients revealed significant correlations (p≤0.05) between egg weight and different external and internal egg quality parameters. Thyroid hormone levels were not significantly different between egg groups on d 6 day, while on d 14, a significant difference was recorded (p≤0.05). In conclusion, larger egg sizes are recommended to obtain better hatchability, lower embryonic death rates, and heavier hatchlings compared with smaller eggs of Japanese quails.
DOI: 10.6084/m9.figshare.10296182.v1
title: Effect of Egg Weight on External and Internal Qualities, Physiological and Hatching Success of Japanese Quail Eggs (Coturnix coturnix japonica)

description: ABSTRACT This experiment was conducted to evaluate the effect of egg weight and egg physical characteristics on embryonic development, hatchability, and hatchling weight of Japanese quails (Coturnix coturnix japonica). In total, 689 eggs were classified in two categories: small (&lt;13.5 g) or large (≥13.5 g), and different external eggshell and internal quality traits were measured. On days 6 and 14 of incubation, tissue triiodothyronine (T3) and thyroxine (T4) were extracted from embryos in both egg sizes and analyzed. Relative to internal egg-quality traits, large eggs had significantly higher yolk height, yolk diameter, yolk weight, albumen height, and albumen weight than small eggs (p≤0.01). However, Haugh unit score (p=0.27) was not significantly different between the two egg sizes. Relative to eggshell quality parameters, large eggs had significantly higher total pore count, surface area, eggshell volume, and eggshell weight than small eggs (p≤0.05), with consequent higher hatchability rate and hatchling weight. Pearson’s correlation coefficients revealed significant correlations (p≤0.05) between egg weight and different external and internal egg quality parameters. Thyroid hormone levels were not significantly different between egg groups on d 6 day, while on d 14, a significant difference was recorded (p≤0.05). In conclusion, larger egg sizes are recommended to obtain better hatchability, lower embryonic death rates, and heavier hatchlings compared with smaller eggs of Japanese quails.
DOI: 10.6084/m9.figshare.10296185
title: Comparison of Several Turning Frequencies During the Storage Period of Red-Legged Partridge (Alectoris rufa) Hatching Eggs

description: ABSTRACT The effects of three egg-turning regimes during the pre-incubation storage period on egg weight loss, hatchability, embryonic mortality, chick weight at hatching and incubation length of red-legged partridge (Alectoris rufa) compared with unturned eggs were investigated. Two hundred eggs were allocated to four 50-egg batches stored at 15ºC and 80% RH that being turned one, four and 24 times a day or remaining unturned, respectively. Eggs were incubated at 37.8ºC and 55% RH during the first 21 days and at 37.5ºC and 75% RH until hatching. Fertility was 70.5% and a good hatchability performance was obtained, characterised by 81.6% hatchability of fertile eggs, and weight losses of 0.78% during storage and of 10.04% during the first 21 days of incubation, 13.6 ±0.1 g hatchling weight, and incubation length of 23.45 ± 0.07 days (mean ± SEM). Hatchability, embryonic mortality developmental stage, egg weight loss during storage and incubation, hatchling weight and length of the incubation period were not affected by the turning frequency or the absence of turning during storage. Higher hatching synchrony was observed for eggs turned four times a day compared with unturned eggs and eggs turned once a day. In conclusion, turning red-legged partridge eggs during medium-term storage periods does not improve egg viability compared with unturned eggs.
DOI: 10.6084/m9.figshare.10296185.v1
title: Comparison of Several Turning Frequencies During the Storage Period of Red-Legged Partridge (Alectoris rufa) Hatching Eggs

description: ABSTRACT The effects of three egg-turning regimes during the pre-incubation storage period on egg weight loss, hatchability, embryonic mortality, chick weight at hatching and incubation length of red-legged partridge (Alectoris rufa) compared with unturned eggs were investigated. Two hundred eggs were allocated to four 50-egg batches stored at 15ºC and 80% RH that being turned one, four and 24 times a day or remaining unturned, respectively. Eggs were incubated at 37.8ºC and 55% RH during the first 21 days and at 37.5ºC and 75% RH until hatching. Fertility was 70.5% and a good hatchability performance was obtained, characterised by 81.6% hatchability of fertile eggs, and weight losses of 0.78% during storage and of 10.04% during the first 21 days of incubation, 13.6 ±0.1 g hatchling weight, and incubation length of 23.45 ± 0.07 days (mean ± SEM). Hatchability, embryonic mortality developmental stage, egg weight loss during storage and incubation, hatchling weight and length of the incubation period were not affected by the turning frequency or the absence of turning during storage. Higher hatching synchrony was observed for eggs turned four times a day compared with unturned eggs and eggs turned once a day. In conclusion, turning red-legged partridge eggs during medium-term storage periods does not improve egg viability compared with unturned eggs.
DOI: 10.6084/m9.figshare.10296170
title: Suppresses of Astragalus Polysaccharide on E. coli-Induced Injured Intestinal Microvascular through TLR4-NF-κB Signal Pathways in Chickens

description: ABSTRACT To investigate the hypothesis that APS can attenuate E. coli-induced microvascular dysfunction in chicken intestine, 60 0-day old male chickens were divided into three groups with 5 replications of 4 chicks. Chicken in the APS group were treated with 15 mg APS daily while the others were given 0 mg APS for 6 days. Then all 7-day old chicken were injected intraperitoneally by E. coli, except for the chicken in the control group. After 4 h, all chicken’s intestinal samples were collected to detect gene expressions involved in inflammatory factors and adhesion molecules. Results showed that APS attenuated the signs of edema and hemorrhage in 7-day old chicken intestinal mucosa which were induced by E. coli injection. Consistently, APS significantly reduced the increasing mRNA levels of inflammatory factors such as Tumor necrosis factor-a (TNF-a), interleukin (IL) -1β and inducible nitric oxide synthase (iNOS) (p&lt;0.05), the same results were observed in vascular adhesion molecules such as E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Moreover, we observed that APS supplementation in water suppressed significantly (p&lt;0.05) the decline of reparative factors such as epithelial growth factor (EGF) and basic fibroblast growth factor (bFGF) in E. coli injected group. Furthermore, supplementation with APS substantially blocked (p&lt;0.05) the increase in Toll-like receptor-4 (TLR4) and Nuclear factor (NF)-κB mRNA abundance (p=0.087) induced by E. coli infection. This study indicated that microvascular injured chicken intestine induced by E. coli would be attenuated with feeding APS, and the mechanism of repairing were probably mediated through TLR4-NF-κB signal pathways.
DOI: 10.6084/m9.figshare.10296170.v1
title: Suppresses of Astragalus Polysaccharide on E. coli-Induced Injured Intestinal Microvascular through TLR4-NF-κB Signal Pathways in Chickens

description: ABSTRACT To investigate the hypothesis that APS can attenuate E. coli-induced microvascular dysfunction in chicken intestine, 60 0-day old male chickens were divided into three groups with 5 replications of 4 chicks. Chicken in the APS group were treated with 15 mg APS daily while the others were given 0 mg APS for 6 days. Then all 7-day old chicken were injected intraperitoneally by E. coli, except for the chicken in the control group. After 4 h, all chicken’s intestinal samples were collected to detect gene expressions involved in inflammatory factors and adhesion molecules. Results showed that APS attenuated the signs of edema and hemorrhage in 7-day old chicken intestinal mucosa which were induced by E. coli injection. Consistently, APS significantly reduced the increasing mRNA levels of inflammatory factors such as Tumor necrosis factor-a (TNF-a), interleukin (IL) -1β and inducible nitric oxide synthase (iNOS) (p&lt;0.05), the same results were observed in vascular adhesion molecules such as E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Moreover, we observed that APS supplementation in water suppressed significantly (p&lt;0.05) the decline of reparative factors such as epithelial growth factor (EGF) and basic fibroblast growth factor (bFGF) in E. coli injected group. Furthermore, supplementation with APS substantially blocked (p&lt;0.05) the increase in Toll-like receptor-4 (TLR4) and Nuclear factor (NF)-κB mRNA abundance (p=0.087) induced by E. coli infection. This study indicated that microvascular injured chicken intestine induced by E. coli would be attenuated with feeding APS, and the mechanism of repairing were probably mediated through TLR4-NF-κB signal pathways.
DOI: 10.6084/m9.figshare.10296134
title: The Role of ß-Mannanase (Hemicell) in Improving Poultry Productivity, Health and Environment

description: ABSTRACT The poultry nutritionists constantly attempt to identify and alleviate the factors responsible for adverse effects on nutrient utilization and production performance in avian species, which are necessary for successful productivity of poultry. Enhanced feed utilization can reduce levels of some nutrients in the diet with concomitant mitigation in nutrient excretion into the environment, reduction of eutrophication and acidification potentials of excreta. Commercial enzymes have been used to improve feed efficiency and utilization in poultry. Among many anti-nutritional factors, the existence of ß-mannans in poultry feed ingredients including soybean and other leguminous seeds has been associated with negative effects on nutrient digestibility and high intestinal viscosity that adversely affects innate immunity and microbial proliferation in poultry gut. The ß-mannanase (a commercial product named as Hemicell) can hydrolyze ß-mannan, an anti-nutritional fiber present in many poultry feed ingredients. Supplement of ß-mannanase to ß-mannan-rich diets may boost the population of intestinal beneficial bacteria, increase the digestibility of mannans, enhance the immunity, suppresses the growth of harmful intestinal bacteria, enhance the digestion and absorption of nutrients in intestinal tracts and reduce the environmental pollution due to poultry excreta. Supplementation of β-mannanase at the level of 200 and 400 mg/kg in poultry diets has positively improved blood glucose and anabolic hormone homeostasis, FCR, digestible energy, and digestible amino acids. This review describes the promising beneficial effects of β-mannanase, which may be used in the poultry feed industry for economic benefits. Another objective of this review is to explore the underlying mechanisms of ß-mannanase that can influence growth, digestion coefficients of nutrients, health and metabolism of nutrients in poultry birds and also the knowledge regarding the useful application of this feed enzyme in the commercial poultry feed industry.
DOI: 10.6084/m9.figshare.10296134.v1
title: The Role of ß-Mannanase (Hemicell) in Improving Poultry Productivity, Health and Environment

description: ABSTRACT The poultry nutritionists constantly attempt to identify and alleviate the factors responsible for adverse effects on nutrient utilization and production performance in avian species, which are necessary for successful productivity of poultry. Enhanced feed utilization can reduce levels of some nutrients in the diet with concomitant mitigation in nutrient excretion into the environment, reduction of eutrophication and acidification potentials of excreta. Commercial enzymes have been used to improve feed efficiency and utilization in poultry. Among many anti-nutritional factors, the existence of ß-mannans in poultry feed ingredients including soybean and other leguminous seeds has been associated with negative effects on nutrient digestibility and high intestinal viscosity that adversely affects innate immunity and microbial proliferation in poultry gut. The ß-mannanase (a commercial product named as Hemicell) can hydrolyze ß-mannan, an anti-nutritional fiber present in many poultry feed ingredients. Supplement of ß-mannanase to ß-mannan-rich diets may boost the population of intestinal beneficial bacteria, increase the digestibility of mannans, enhance the immunity, suppresses the growth of harmful intestinal bacteria, enhance the digestion and absorption of nutrients in intestinal tracts and reduce the environmental pollution due to poultry excreta. Supplementation of β-mannanase at the level of 200 and 400 mg/kg in poultry diets has positively improved blood glucose and anabolic hormone homeostasis, FCR, digestible energy, and digestible amino acids. This review describes the promising beneficial effects of β-mannanase, which may be used in the poultry feed industry for economic benefits. Another objective of this review is to explore the underlying mechanisms of ß-mannanase that can influence growth, digestion coefficients of nutrients, health and metabolism of nutrients in poultry birds and also the knowledge regarding the useful application of this feed enzyme in the commercial poultry feed industry.
DOI: 10.6084/m9.figshare.10296149
title: Replacement of Animal Fat by Canola Oil in Chicken Meatball

description: ABSTRACT This work aimed to develop chicken meatballs with five levels of animal fat replacement by canola oil and to evaluate the product’s behavior during 120 days of storage. For that, analyzes were performed in the centesimal composition, fatty acid profile, shelf life and sensory parameters. The centesimal composition of the product did not present significant difference (p&gt;0.05) between the treatments. However, canola oil addition improved the lipid profile, atherogenic and thrombogenic indexes, and the ratios of PUFAs/SFAs (0.91 to 1.61) and n6/n3 (12.63 to 3.74) in the products. Animal fat replacement and storage time also reduced meatballs Aw and pH. No differences in texture between treatments were detected after 30 days of storage. The lipid oxidation presented by mean values of TBARs ranged from 0.71 and 1.35 mg MDA/kg, indicating the onset and the regression of lipid oxidation. The color parameters exhibited a spectrum towards the luminous yellow color, predominant in both chicken meat and canola oil. The results of this study indicate that the substitution of animal fat by canola oil is promising in the production of meat products with good acceptability (70%) during the sensory analysis.
DOI: 10.6084/m9.figshare.10296149.v1
title: Replacement of Animal Fat by Canola Oil in Chicken Meatball

description: ABSTRACT This work aimed to develop chicken meatballs with five levels of animal fat replacement by canola oil and to evaluate the product’s behavior during 120 days of storage. For that, analyzes were performed in the centesimal composition, fatty acid profile, shelf life and sensory parameters. The centesimal composition of the product did not present significant difference (p&gt;0.05) between the treatments. However, canola oil addition improved the lipid profile, atherogenic and thrombogenic indexes, and the ratios of PUFAs/SFAs (0.91 to 1.61) and n6/n3 (12.63 to 3.74) in the products. Animal fat replacement and storage time also reduced meatballs Aw and pH. No differences in texture between treatments were detected after 30 days of storage. The lipid oxidation presented by mean values of TBARs ranged from 0.71 and 1.35 mg MDA/kg, indicating the onset and the regression of lipid oxidation. The color parameters exhibited a spectrum towards the luminous yellow color, predominant in both chicken meat and canola oil. The results of this study indicate that the substitution of animal fat by canola oil is promising in the production of meat products with good acceptability (70%) during the sensory analysis.
DOI: 10.6084/m9.figshare.10296146
title: Evaluation of the Relationship Between the Breeder’s Age, Breed and the Incubator Types on Quality and Animal Welfare in the Hatchery

description: ABSTRACT About 33.3% of chicken life is conditioned by the result of embryonic development. Therefore, understanding and improving the early phase of the broiler’s life is critical to guarantee their well-being. Thus, the objective of this research was to evaluate the relationship between the breeder’s age, breed and the incubator types on quality and animal welfare in the hatchery. The experimental design was completely randomized in a 2 x 2 x 3 factorial arrangement (incubator, breed and age), with 12 treatments and 6 replicates each. The eggs derived of Cobb 500 and Ross’s breeders at the beginning of the laying period, middle age and at the end of the laying period. It is concluded that the size of the eggs is related to the breeder’s age and breed, which influences directly the number of bled eggs. In addition, well-being in the hatchery can be affected by decreased hatching and contamination that is higher in older breeders. Cobb eggs also need more attention because they are larger, therefore, they have a higher risk of breakage and they are more sensitive to the low concentration of CO2 at the hatcher.