cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/73vmpz894x.2
title: RNAi of <i>nhx-2</i> impedes reproductive senescence and aging via restoring mitochondrial membrane depolarization in <i>C. elegans</i>

description: Neurodegenerative diseases do not have a complete cure yet. The strategies that target overall healthy aging might be very effective in treating such debilitating conditions. Reproductive senescence (RS) is one such phenomenon which could be associated with healthy aging vis-à-vis neurodegeneration. Here, we have employed bioinformatics, functional genomics, and molecular biology approaches to understand the mechanistic association between RS and NDs. Employing RNAi induced silencing we performed a systematic screening of subset of 22 such genes that delay reproductive senescence; on the basis of their effect on the various endpoint of neurodegeneration including α-synuclein aggregation, ROS level, locomotion and effect of estradiol upon the functioning of these genes in transgenic strain NL5901 of C. elegans. Our studies led to the identification of a Na+/H+ exchanger as its silencing had a profound effect on endpoints associated with PD. Further, we performed an in silico analysis and found out that the estradiol receptor is having an affinity towards direct inverse repeats of upstream region of nhx-2. We treated worms with 17 beta-estradiol and it gave rise to the altered mRNA levels of nhx-2. We further observed that RNAi of nhx-2, prolonged reproductive span of the worms by 71.3%. Knockdown of nhx-2 is known to alter pH of the gut so we endeavored to decipher whether the observed effects are correlated with selective food absorption. Performing network analysis, immunoblotting and fecundity assay we found out that nhx-2 RNAi is actually working via SIR-2.1 and in absence of SIR-2.1 effects exerted via nhx-2 are withdrawn. When we performed a co knockdown of sir-2.1 and nhx-2, RNAi of nhx-2 did not alter the fecundity of C. elegans which proves that it is showing its effect via altering the levels and functionality of SIR-2.1. We observed that nhx-2 is actually giving rise to selective food absorption and that is mimicking calorie restriction which in turn altering the levels of SIR-2.1.In order to check the mechanistic insight we blindly performed a real-time analysis of genes associated with several pathways and we found that nhx-2 RNAi is affecting genes of the mitochondrial pathway. We also checked the mitochondrial membrane potential of individual cells of worm sine nhx-2 is a proton exchanger and we found that it also improves mitochondrial health by restoring the depolarization of mitochondrial membrane potential. Hence, knockdown of nhx-2, impedes reproductive senescence, extends healthy reproductive span and ameliorates effects associated with neurodegeneration. The data here substantiates the link between reproductive senescence with aging and neuronal health via genes that can alter all over the reproductive health of C. elegans.
DOI: 10.17632/cg4zg75mgs.1
title: In Utero Exposures to Electronic-Cigarette Aerosols Impair the Wnt Signaling during Mouse Lung Development - Online Supplement - Uncropped Western Blots

description: Uncropped blots. All primary and secondary antibodies were obtained from Cell Signaling Technology Inc., Danvers, MA, USA (WNT signaling antibodies sampler kit, Cat. # 2915). The Precision Plus Protein All Blue Standards (10–250 kD) (Cat. #1704159, Bio-Rad) markers were used. The markers were detected only at red wavelengths (635 nm laser or red LED). A) Uncropped pLRP6 blot. B) Uncropped LRP6 blot. C) Uncropped DVL2 blot. D) Uncropped DVL3 blot. E) Uncropped WNT5A/B blot. F) Uncropped β-ACTIN blot.
DOI: 10.17632/cg4zg75mgs.2
title: In Utero Exposures to Electronic-Cigarette Aerosols Impair the Wnt Signaling during Mouse Lung Development - Online Supplement - Uncropped Western Blots

description: Uncropped blots. All primary and secondary antibodies were obtained from Cell Signaling Technology Inc., Danvers, MA, USA (WNT signaling antibodies sampler kit, Cat. # 2915). The Precision Plus Protein All Blue Standards (10–250 kD) (Cat. #1704159, Bio-Rad) markers were used. The markers were detected only at red wavelengths (635 nm laser or red LED). A) Uncropped pLRP6 blot. B) Uncropped LRP6 blot. C) Uncropped DVL2 blot. D) Uncropped DVL3 blot. E) Uncropped WNT5A/B blot. F) Uncropped β-ACTIN blot.
DOI: 10.17632/sjpjybxmfp.1
title: Original data in "Stabilization of Fast Pyrolysis Liquids from Biomass by Mild Catalytic Hydrotreatment: Model Compound Study"
DOI: 10.17632/zwpzhfcjwb.2
title: Superposed deformation in the Península Mitre recess (Fuegian thrust-fold belt, southern Argentina), and implications for orogenic curve kinematics

description: Raw database of fault data used for the paleostress determinations in Torres Carbonell et al. (2019), https://doi.org/10.1016/j.jsg.2019.103947. For comparison, sites used by Maestro et al. (2019) are shown. The shaded cells are the ones that explain the solutions given in Table 1 and Fig. 11, after filtering according to the methodology explained in section 4 of the manuscript. Bedding is given as dip direction/dip.
DOI: 10.34677/1728-7936-2019-6-14-18
title: ORGANIC CULTIVATION TECHNOLOGY OF ECOLOGICALLY PURE POTATOES OF EARLY VARIETIES
title: ОРГАНИЧЕСКАЯ ТЕХНОЛОГИЯ ВОЗДЕЛЫВАНИЯ ЭКОЛОГИЧЕСКИ ЧИСТОГО КАРТОФЕЛЯ РАННЕГО

description: One of the technologies of obtaining clean ecological products is organic farming. To obtain environmentally friendly early production of potatoes, the authors analyzed the practical use of such techniques as covering potato plantings in the initial growth period, decapitation, germination of tubers before planting and the combined use of covering and germination, which provide for fuller utilization of the crop growing capacity. Agronomic grounds for the use of technological methods were provided in 2015-2019 for the Udacha variety. The experiments were repeated 3 times. The cropping pattern used was 70×35 cm. Harvesting was carried out in 2 periods: July 15 and July 30. Statistical processing of the results was carried out by B.A. Dospekhov’s
method of variance analysis. The content of heavy metals and chemical composition was determined by standard methods. It was found that when using the aforementioned technological methods, the yield increases by 10.2…23.2% as compared with the control variant, depending on the dates of harvesting. The minimum increase in yield (10.2%) was observed in the variant with the germination of tubers before planting, the maximum increase (16.1%) in the variant with a combined use of germination and covering. When harvesting tubers in the second period (July 30), the trend remained, but the increase was higher and ranged
from 15.3 to 23.3%. As to the chemical composition of the fi ve variants of experimental samples of potato tubers, no differences were found. The study proved the expediency of using the technological methods to obtain early potato yield without changing the chemical composition and ensure the content of various heavy metals within acceptable standards.
DOI: 10.34677/1728-7936-2019-6-19-22
title: SUBSOIL IRRIGATION OF CRIMEAN VINEYARDS WITH DESALINATED CONDENSATE OF SEA AND SALT LAKE WATER
title: ПОДПОЧВЕННОЕ ОРОШЕНИЕ ВИНОГРАДНИКОВ КРЫМА ОПРЕСНЁННЫМ КОНДЕНСАТОМ МОРСКОЙ И СОЛЁНОЙ ОЗЁРНОЙ ВОДЫ

description: The paper discusses the use of salt water for irrigation of perennial crops in areas adjacent to water sources with high salt content, including sections of the coast. The authors give a brief analysis of various ways to reduce the salt content in water. Particular attention is paid to the removal of salts by distillation of salt water using solar energy. Various designs of solar desalination plants patented in different countries are considered. The authors propose a method for subsoil irrigation of perennial crops with salt water, which consists in evaporating water using solar energy, saturating the air with steam and then supplying it through perforated pipes to the soil where steam condensation occurs. To implement this method, a special device has been
developed. The effectiveness of the application of this method and device in agricultural production is considered using an  example of the Crimea vineyards. It has been established that the subsoil water supply will reduce moisture loss by 20…30% as compared to drip irrigation by preventing evaporation from the surface of a wet spot near the dropper. Due to this, the irrigation rate can be reduced to 400…500 m3
/ha or up to 50 liters per 1 m2 . Implementation of the proposed method will allow irrigating
perennial crops with desalinated salt water and prevent salt accumulation in the soil and the loss of water by evaporation.
DOI: 10.34677/1728-7936-2019-6-23-27
title: FEATURES OF BIOFUEL APPLICATION IN AGRICULTURAL PRODUCTION
title: ОСОБЕННОСТИ ПРИМЕНЕНИЯ БИОТОПЛИВА В СЕЛЬСКОХОЗЯЙСТВЕННОМ ПРОИЗВОДСТВЕ

description: The paper provides study results on the properties of biofuels based on methyl ethers of vegetable oils. Using an automatic analyzer, the author determined the fractional composition of biofuel. The content of free water in ethers when assessing hygroscopicity was determined by coulometric titration according to the Fisher method. The compatibility of these products with various structural materials was determined. It was found that the fractional composition of methyl ethers of rapeseed and sunfl ower oil differs signifi cantly from the indicators of commercial diesel fuel and is characterized by a narrow range of fractionation. This makes it diffi cult to use pure ethers instead of diesel fuel, so it is advisable to use these products in the form of mixtures
with diesel fuel. When ethers come into contact with rubber parts of fuel system equipment, rubber swelling and the destruction of some parts are observed. The mass of rubber samples contacting for 12 days with ethers of vegetable oils increased by 18…25%, and the surface area – by 12…17%. It is proposed that gasket and sealing parts of equipment designed for operation with ethers should be replaced with tetrafl uoroethylene (fl uoroplast), etc. It is established that protective coatings made of phenolalkide enamel ФА-5278 do not withstand contact with ethers. Since ethers of vegetable oils are hygroscopic, it is recommended that
they should be stored in closed containers to prevent their contact with moisture.
DOI: 10.34677/1728-7936-2019-6-28-34
title: ORGANIZATION OF WORKPLACE AND LABOR PROCESS OF TRACTOR DRIVER IN MODERN MOBILE MACHINES USED IN AGRICULTURE
title: ОРГАНИЗАЦИЯ РАБОЧЕГО МЕСТА И ТРУДОВОГО ПРОЦЕССА ТРАКТОРИСТА-МАШИНИСТА В СОВРЕМЕННЫХ МОБИЛЬНЫХ МАШИНАХ ДЛЯ СЕЛЬСКОГО ХОЗЯЙСТВА

description: The paper discusses some issues of changes in and specifi cation of basic requirements for the organization of workplaces and labor process of farm tractor drivers in cabins of modern tractors and combines. It is shown that the introduction of computer control systems of the tractor and its engine, machine-tractor units and combines, dispatching operations performed in the fi eld in an online mode with the use of global satellite and local (unmanned aerial vehicles-UAVs) control and regulation systems requires a new approach to the organization of the workplace and the process of work and rest of tractor drivers. The authors carried out an analysis of possible problems and outlined conditions for health maintenance of machine operators working under conditions of operation computerization, as well as the use of video terminals and UAVs. It is shown that the documents regulating sanitary rules relation to the design and operations of tractors and agricultural machines and “Instructions on the hygienic assessment of tractors and agricultural machines” need to be modifi ed taking into account the actual technological changes. Recommendations are also given to increase
attention to the peculiarities of labor protection of machine operators, taking into account changes in their working process.
DOI: 10.34677/1728-7936-2019-6-35-39
title: TECHNIQUE OF SELECTING THE OPTIMUM MODES OF ELECTROCONTACT WELDING
title: МЕТОДИКА НАЗНАЧЕНИЯ ОПТИМАЛЬНЫХ РЕЖИМОВ ЭЛЕКТРОКОНТАКТНОЙ ПРИВАРКИ

description: The authors developed a technique of selecting rational parameters of electrical contact welding (ECW) to ensure the quality of coatings produced on the parts of agricultural machines during their restoration and/or hardening. They determined factors that have the greatest infl uence on the quality and properties of coatings – the ECW mode parameters: compression force of welding electrodes, welding current, welding pulse and pause duration, welding speed, material feed, or feed rate, and coolant fl ow rate. By means of the proposed method, the ploughshare from 65Г steel of GOST 1133-71 was strengthened with coating from У12A steel of GOST 2283-79. The following parameters of the EPW mode were obtained: a welding current value of 6.9 kA, a pulse time of 0.06 s, a pause duration of 0.08 s, and a coolant fl ow rate of 1.8 l /min, the overlap coeffi cient of weld spots in a row of 0.635, the overlap coeffi cient of weld spots between rows of 0.76, the coating area welded per pulse of 6.49 mm2, a welding speed of 0.986 m/min and the maximum performance of the ECW process of 22.48 cm2/min. The hardness of the obtained coatings was 60…62 HRCэ, and the relative wear resistance was more than 4 times higher than that of steel 45 (180 HB). The study has experimentally confi rmed the feasibility of using the developed technique for selecting rational EPW parameters to ensure
the quality of coatings produced on the parts of agricultural machines during their restoration and/or hardening.
description: Разработана методика назначения рациональных параметров электроконтактной приварки (ЭКП) для обеспечения качества покрытий на деталях сельскохозяйственных машин при их восстановлении и/или упрочнении. Установлены факторы, оказывающие наибольшее влияние на качество и свойства покрытий – параметры режима ЭКП: усилие сжатия
сварочных электродов, величина сварочного тока, длительность сварочного импульса и длительность паузы, скорость сварки, подача или скорость подачи, расход охлаждающей жидкости. С использованием предлагаемой методики было произведено упрочнение лемеха плуга из стали 65Г ГОСТ 1133-71 нанесением покрытия из стали марки У12А ГОСТ 2283-79. Получены следующие параметры режима ЭКП: величина сварочного тока 6,9 кА, время импульса 0,06 с, продолжительность паузы 0,08 с и расход охлаждающей жидкости 1,8 л/мин, коэффициент перекрытия сварочных точек в ряде 0,635, коэффициент перекрытия сварочных точек между рядами 0,76, площадь покрытия привариваемого за импульс 6,49 мм2, скорость сварки 0,986 м/мин и максимальная производительность процесса ЭКП 22,48 см2/мин.
При этом твёрдость полученных покрытий составила 60…62 HRCэ, а относительная износостойкость более чем в 4 раза выше, чем у стали 45 (180 HB). Экспериментально подтверждена целесообразность применения разработанной методики назначения рациональных параметров ЭКП для обеспечения качества покрытий на деталях сельскохозяйственных машин при их восстановлении и/или упрочнении.
DOI: 10.34677/1728-7936-2019-6-4-9
title: DETERMINING THE PARAMETERS OF A AUGER LOADING MIXER OF ACTIVATED COMPOST
title: ОБОСНОВАНИЕ ПАРАМЕТРОВ ШНЕКОВОГО ПОГРУЗЧИКА-СМЕСИТЕЛЯ ОРГАНОМИНЕРАЛЬНОГО КОМПОСТА

description: The mixing quality of activated compost is determined by the operating parameters of working elements. The paper presents a design of an activated compost loading mixer equipped with a belt auger and a metering hopper for mineral fertilizers. The authors provide basic research results on interaction process of the auger with a compost mixture and determine the basic design and operating conditions of the considered actuating elements. They obtained formulas to determine the optimal travel speed of the activated compost loading mixer; the rotational speed and diameter of the separating teeth of the auger, as well as the number of teeth. The study proved the relationship between the auger capability of separating a portion of material from
the main mass and its rotational speed and diameter, as well as the relationship between the transportation rate and the factor determined by the resistance of racks holding the teeth. The experimental study results have proved that the best mixing ratio value of 0.96 is achieved at a loader travel speed of 0.05 m/s and a rotational speed of the auger of 175…215 min-1, and the maximum performance value amounts to 39 kg/s at a travel speed of 0.05 m/s and an angular speed of the auger of 175…15 min-1.
DOI: 10.7910/dvn/0nlh95/5zkxzu
title: w2trial1.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/9errmo
title: w2trial5.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/fddbqj
title: w2trial4.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/ixdalu
title: w1trial1.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/lnkgni
title: w5trial5.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/lr8h2z
title: w1trial1.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/o6o3wx
title: w1trial1.ply

description: :unav
DOI: 10.7910/dvn/0nlh95/p5g1zp
title: w2trial3.ply

description: :unav
DOI: 10.17632/4hm7n3b5d9.1
title: Improved Survival in Women versus Men with Merkel Cell Carcinoma

description: Studies have observed that women have better outcomes than men in melanoma, but less is known about the role of sex differences in outcomes for other aggressive cutaneous malignancies. We hypothesized that similar sex differences may be observed in Merkel cell carcinoma. In this study, including over 4,100 patients with Merkel cell carcinoma undergoing definitive surgical resection in the National Cancer Data Base between 2004 and 2014, we report that women had a consistent survival advantage in comparison to men after adjusting for other covariates in multivariate Cox regression and propensity score matched cohorts. Improved outcomes were observed in women across virtually all subgroups of patients. In this same study, we validated the initial results by using the SEER database. Importantly, we observed the improved survival in women was completely attributable to increased cancer-specific mortality, whereas there was no difference in other-cause mortality.
DOI: 10.17632/v36wkmdc9p.1
title: Negative effects of authentic learning experience with e-learning on intentions to use it for teaching

description: The data were obtained by using the Theory of Planned Behavior (TPB) questionnaire to predict the changes in intentions to use e-learning for teaching among low-performing preservice EFL teachers after they received authentic learning with e-learning.
DOI: 10.17632/v36wkmdc9p.2
title: Negative effects of an authentic learning experience with e-learning on low-performing students’ intentions to use e-learning for teaching

description: The data were obtained by using the Theory of Planned Behavior (TPB) questionnaire to predict the changes in intentions to use e-learning for teaching among low-performing preservice EFL teachers after they received authentic learning with e-learning.
DOI: 10.34677/1728-7936-2019-6-40-45
title: METHOD OF RESTORING THE WORN SURFACE OF A REDUCTION GEAR SHAFT IN A ‘SHAFT-TO-COLLAR’ CONNECTION USING A REPAIR BUSHING AND POLYMERIC MATERIALS
title: СПОСОБ ВОССТАНОВЛЕНИЯ ИЗНОШЕННОЙ ПОВЕРХНОСТИ ВАЛА РЕДУКТОРА В СОЕДИНЕНИИ «ВАЛ-МАНЖЕТА» С ПРИМЕНЕНИЕМ РЕМОНТНОЙ ВТУЛКИ И ПОЛИМЕРНЫХ МАТЕРИАЛОВ

description: The authors prove the feasibility of applying a method for restoring the worn surface of a reduction gear shaft in a “shaftsleeve” connection using a repair bushing and a polymer material. It is recommended to use selective assembly with a transition fi t with the maximum possible interference fi t of 0.02 mm, and the maximum possible clearance of 0.16 mm. The experimental samples of repair bushings are made of thin-walled high-quality pipes made of steel 30ХГСА GOST 8734-75. The sleeve was fi xed on the shaft using the Unigerm-6 anaerobic polymer composition. An analysis of the test results showed that the shear strength does not correspond to the manufacturer’s data for the considered size of the fi t, but the strength values are higher than permissible ones. The dispersion of the obtained force values is not large and correlates with the clearance sizes in the samples. The study confi rmed the composition polymerization in a clearance of approximately 0.3 mm. The recommended maximum possible clearance of 0.16 mm ensures suffi cient strength of the coupling and the centering accuracy of the workpiece on the shaft (at the maximum possible shaft runout of 0.18 mm). To ensure tightness in the fi t, it is proposed to heat the bushing before
installation to 150°C, which is suffi cient enough to obtain an interference fi t but does not affect the properties of the polymer composition.
DOI: 10.34677/1728-7936-2019-6-46-52
title: OBSTACLES TO INNOVATIVE AND TECHNOLOGICAL DEVELOPMENT OF RUSSIAN AGRICULTURE
title: ЧТО ТОРМОЗИТ ИННОВАЦИОННОЕ И ТЕХНОЛОГИЧЕСКОЕ РАЗВИТИЕ РОССИЙСКОГО АПК

description: Russian economy and agriculture, in particular, are noticeably lagging behind in innovative and technological development. The aim of the study is to identify the causes of this lag and offer proposals to overcome it. The author outlines a conceptual scheme of organizing the promotion of imported innovative products on the market, which is distinguished by its complexity and high controllability on a global scale. The study has identifi ed the dependence of many Russian farmers on foreign technologies, which poses a threat to national food security. The main reasons for the competitive loss of domestic innovations and the technological
backlog of agriculture include: a closed system for organizing scientifi c research; lack of funding for scientifi c research; lack of an extensive technology transfer system; low profi tability of agricultural producers. Measures are proposed to overcome the innovative and technological lag, including establishing modern institutions of innovative development, in particular, “technological valleys”; providing effective information and consulting services; redistribution of state fi nancing in favor of those  who produce competitive scientifi c products developed into technological solutions; organization of the training of innovationoriented specialists in educational institutions; implementation of package offers for arm producers through the agricultural
leasing system; launching a national agricultural extension system; normalization of economic relations of farm enterprises with their counterparties. Conclusion is drawn about the possibility of overcoming the innovation and technological backwardness of Russian agriculture on condition a range of organizational decisions are made and relevant state support is provided.