cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.402
title: Production and cytotoxicity against cancer cells of recombinant cecropin b-polyproline

description: Cecropin B (CB) is a natural anticancer antimicrobial peptide which has the highest antibacterial activity among the Cecropin family. In this work, Cecropin B - polyproline (CB-PP) was constructed by adding polyproline (PP) which is a part of progesterone receptor (PR) to the C-terminus of CB sequence. The CB and CB-PP gene fragments were cloned into the pPICZαA expression vector. Then, the recombinant plasmid CB and CB-PP were transformed into Pichia pastoris strain KM71H by electroporation. The recombinant protein CB (rCB) and CB-PP (rCB-PP) were successfully produced by inducing with 0.5% methanol for 24 and 48 hours and purified using nickel column chromatography. The size of rCB and rCB-PP were found to be approximately 4.9 and 6.6 kDa, respectively. The cytotoxicity against six cell lines including human breast carcinoma (BT-474), human lung bronchus carcinoma (ChaGo-K1), human liver hepatocellular carcinoma (Hep-G2), human gastric carcinoma (Kato-III), human colon carcinoma (SW-620) and human lung fibroblast (WI-38) were investigated. All cell lines were treated with recombinant protein CB and CB-PP with the protein concentration between 0.078 µM – 20 µM for 72 hours. The results revealed that more than 50% of those five cancer cells were dead at the rage of 7 µM - 20 µM protein concentrations while more than 80% of normal cells was alive even treated with the same protein concentration. Those resultsmindicated that rCB และ rCB-PP could be produced by the transformed Pichia pastoris and the obtained recombinant proteins exhibited anticancer activity.
DOI: 10.14457/cu.the.2015.403
title: Framework for laptop review analysis

description: This research aims to develop a framework which enables users to be aware that what the reviews on the laptop mentioned. The objective of the research is to propose the framework for the analysis of laptop reviews. The framework consists of four main processes: preparing data for analysis, detecting subjective text paragraphs, identifying the aspect of each text paragraph and classifying the sentiment of each text paragraphs. The subjective text paragraphs are found by detecting subjective words in the sentences of each paragraph. Then, only the subjective paragraphs will be categorized into each category by comparing with the vocabulary in each aspect domain. Finally, the sentiment of paragraphs will be classified into positive and negative opinions by the Naïve Bayes classifier. The test results show that the accuracy of the subjective detection and the aspect identification of the text paragraph are more than 90% and the accuracy of sentiment classification is more than 77%. In summary, this framework is helpful in the development of the analysis system of reviews on a laptop to help consumers in making a decision before purchasing.
DOI: 10.14457/cu.the.2015.404
title: Combining neighborhood-based and model-based on multi-criteria recommendation

description: Recommender system is a tool invented to filter information that seeks to provide personalized recommendations. The traditional recommender system makes the recommendations using the overall preferences toward items provided by the users. However, the multi-criteria recommender system suggests that the overall preferences of each individual user can be affected by his unequal personal interest in some criteria of the items. Learning such effect of each criterion becomes the key to produce more personalized recommendations. Most of methods in recommender systems are based on the neighborhood-based or the model-based techniques. To improve the performance of the recommendation, both techniques are often aggregated together. In this work, a novel multi-criteria recommendation technique is proposed. The prediction from each criterion is made by considering the trade-off between the neighborhood-based and the model-based techniques. The effects if the criterion ratings to the overall rating are measured by the similarities among the user preference patterns, extracted from matrix factorization. The overall rating is then predicted by weighted averaging the predictions from all criteria, using such criteria effects as the weights. The evaluation shows that our proposed method outperforms various well-known techniques on both single and multi-criteria recommendation.
DOI: 10.14457/cu.the.2015.405
title: Effects of nitrogen addition on vibrational property of high nitrogen-content GaPN thin films

description: Structural, vibrational and optical properties of GaP1-xNx films with N contents (x) up to 5.4 at% grown on GaP (001) substrates by MOVPE have been investigated by high resolution X-ray diffraction (HRXRD), micro-Raman spectroscopy and micro-photoluminescence (micro-PL). An average N content was verified by HRXRD to be in a range of 0 to 5.4 at%. All the films are under tensile strain. Smooth surface and fairly flat interface were confirmed by atomic force microscopy (AFM) and scanning electron microscopy (SEM), even though the N atoms were incorporated as high as 5.4 at%. Raman spectra showed the N-related vibrational modes (N-VMs) in range of 440 – 520 cm-1, which is the first validation for GaPN at room temperature. We have investigated the N-VMs Raman intensity (IN-VMs) as a function of N content determined by HRXRD (xXRD). The IN-VMs was found to rise for the GaPN films with higher N incorporation. It is also evident that the N content in the GaPN films determined by Raman spectroscopy technique (xRaman) exhibits a linear dependence on the xXRD. Our results demonstrate that the linear dependence of xRaman on the xXRD provides a useful calibration method to determine the N content in dilute GaPN films with the N content up to 5.4 at%. Room temperature bandgap determined by micro-PL is dramatically reduced, when the N content is increased. A huge band gap bowing parameter of GaPN is calculated to be 10 eV.
DOI: 10.6084/m9.figshare.13646756
title: Table S2.xlsx

description: Calculated paleotemperature values by GDGTs for the QB sediments in Cenozoic era.
DOI: 10.14457/cu.the.2015.406
title: Selection of bacterial community on electrode by electric current for use as microbial fuel cell anode

description: This dissertation studied the effect of anode electric current stimulation on open circuit voltage (Voc), current density and power density of MFC. Parameters which were used for electric current stimulation were stimulation periods, enrichment medium, carbon source in MFC, type and magnitude of electric current, and source of sediment. In addition, effect of nutrient broth (NB) and phosphate buffer basal medium (PBBM) on biofilm formation of stimulated anode were examined. Biofilm formation on anode was investigated under field emission scanning electron microscope (FESEM). It was found that thick biofilm was observed on AC stimulated anode at 10-15 mA. In addition, the different medium used in the enrichment of biofilm during AC stimulation led to the different characteristics of biofilm. Bacterial community on stimulated anode was isolated from biofilm anode. Electrochemical active bacteria (EAB) such as Shewanella putrefaciens, which effectively transferred electron to anode was isolated. The effect of AC stimulation on pure isolate showed that S. putrefaciens viable cell count was dramatically decreased (4 logCFU) when stimulated with 15 mA AC stimulation. In this dissertation, the highest Voc of 989 mV, produced from propionate-fed MFC with 60 days unstimulated anode in PBBM medium. However, the highest current density of 72.9 mA and power density of 13.4 mW m-2, produced from acetate-fed MFC with 60 days, 5-10 mA AC stimulated anode in PBBM medium. Moreover, AC stimulation on anode can be used to stimulate different sources of sediments. Furthermore, application of stimulated anode for COD removal and electricity production was also investigated. It was found that the highest COD removal of 60% and 48 mA m-2 current density was obtained from molasses-fed MFC by using 10 mA AC stimulated anode. Thus, it could be concluded that electric current stimulation is a high potential tool for selecting effective bacterial community on electrode and using as anode for the MFC.
DOI: 10.6084/m9.figshare.13646756.v1
title: Table S2.xlsx

description: Calculated paleotemperature values by GDGTs for the QB sediments in Cenozoic era.
DOI: 10.14457/cu.the.2015.407
title: Pseudomonas cepacia lipase immobilized onto expandable polystyrene beads for biodiesel production

description: In previous results of our research group, the lipase from Pseudomonas cepacia was immobilized onto polyglutaraldehyde-styrene copolymer coated expandable polystyrene beads (PGlu-STY/EPS) with only one particle size. There was a report that the maximum enzymatic reaction rate was at a higher level by using lipase-immobilizing with smaller particle size. To improve the efficiency of lipase-immobilized catalyst for biodiesel production, the smaller particle sizes of PGlu-STY/EPS beads were prepared by varying coating temperature and the beads prepared however were unsuitable for immobilization of the lipase. Thus two sizes of expandable polystyrene bead (EPS) consisting of EPS bead grade 291L, with size of 1.00-1.60 mm, and EPS bead grade 321F, with size of 0.63-1.12 mm, were used for coating with PGlu-STY copolymer and the lipase-immobilized catalysts were then evaluated their lipase activity and efficiency for biodiesel production from soybean oil. During coating process, it was found that PGlu-STY/EPS beads not only could be prepared by using soluble polyglutaraldehyde as described in the literature but also the whole suspension of polyglutaraldehyde generated from the same original concentration of the glutaraldehyde. Using the whole suspension of polyglutaraldehyde gave the higher percentage of coating PGlu-STY on both sizes of EPS beads than that of soluble polyglutaraldehyde. In both sizes of EPS beads, the lipase-immobilized catalysts that was prepared by using the soluble polyglutaraldehyde provides higher activity than that was prepared by using the whole suspension of polyglutaraldehyde and the activity of lipase immobilized on 321FEPS bead was higher than 291LEPS bead. The efficiency of those immobilized catalysts for biodiesel production from soybean oil was notably low. Nevertheless, the addition of water to the reaction could significantly improve production of biodiesel. Furthermore, the lipase-immobilizing with small size which was prepared by using the soluble and the whole polyglutaraldehyde has better efficiency than the bigger one.
DOI: 10.14457/cu.the.2015.408
title: In vitro screening techniques for Thai herbal extracts on alzheimer's disease prevention

description: Alzheimer's disease (AD) is a neurodegenerative disorder that is commonly found in elderly people. The major causative factor of AD is an extracellular accumulation and aggregation of amyloid β-peptide (Aβ) which results from the imbalance between its production and clearance in the brain. Thus, the potential strategies in the battle against AD focus on the discovery of multifunctional agents, especially from the natural products, which can act against multiple pathogenic steps of Aβ. Therefore, the screening system was set up in this study and nine Thai herbs; Caesalpinia sappan Linn., 1753 (CS), Thunbergia laurifolia Lindl., 1867 (TL), Rhinacanthus nasutus (Linn.) Kurz., 1870 (RN), Tabernaemontana divaricata (Linn.) R.Br. ex Roem. & Schult., 1819 (TD), Cyperus rotundus Linn., 1753 (CR), Terminalia chebula Retz., 1788 (TC), Azadirachta indica A. Juss., 1830 (AI), Piper retrofractum Vahl., 1804 (PR), and Asparagus racemosus Willd., 1799 (AR) were selected for the screening based on their properties for remedy the cognitive symptoms in ethnopharmacopoeia and have been researched in the fields of neurological disorders. The stems, leaves, roots or fruits of these herbs were extracted and then performed the 2 consecutive steps of the in vitro test; biochemical- and cell-based assay. Anti-Aβ aggregation, Aβ fibril disaggregation and antioxidant potencies of the extracts were investigated by the biochemical-based assay. The results indicated that, except AR, all eight herbal extracts had potential to inhibit Aβ aggregation and stimulate Aβ fibril disaggregation, and also exhibited high antioxidant activity. Hence, the next step of the investigation using cell-based assay was conducted, and the protective effect of these herbal extracts on Aβ-induced neuronal toxicity in SH-SY5Y neuroblastoma cell was determined. Among the eight herbal extracts, only RN, TD, TC and PR effectively protected against Aβ-induced neurotoxicity. Therefore, these four herbal extracts were then employed to investigate the modulating potential on the expression of genes associated with Aβ production (App, Bace1 and Adam10) and clearance (Ide and Nep) in SH-SY5Y cells. The result showed that PR had a potential to suppress Aβ production by decreasing β-secretase Bace1 and increasing α-secretase Adam10 mRNA expression levels, respectively, while TC had a potential to enhance the degradation of Aβ by increasing mRNA expression level of Ide. Altogether, this study indicates that PR and TC possibly particular candidate herbal medicines for developing the multifunctional anti-AD agents for human use in the future. In addition, this screening system has been suggested to be applied as a standardized tool to screen the high potential Thai herbs on AD therapy at a commercial and pharmaceutical scale.
DOI: 10.14457/cu.the.2015.409
title: Surface modification of CdSe-ZnS quantum dots with phospholipid from oil-seed camellia Camellia oleifera Abel.

description: In this work, we studied the preparation of water-dispersible fluorescent CdSe-ZnS core-shell quantum dots (QDs) using the surface modification with natural amphiphilic phospholipids (PLs) and investigated the stability and cytotoxicity of the results QDs. We isolated the amphiphilic phospholipids from extra virgin camellia (Camellia oleifera Abel.) oil by degumming process using acid treatment with 85% phosphoric acid. The results showed that the highest percent yield of the camellia phospholipids was 1.69% w/w of total extra virgin of camellia oil. The functional groups of camellia phospholipids comprised C-H bonding, carbonyl group(C=O), and PO2 as characterized by Fourier transform infrared spectroscopy, Proton nuclear magnetic resonance spectroscopy (1H-NMR) and 31-Phosphorus Nuclear magnetic resonance spectroscopy (31P-NMR). The structures of camellia phospholipids were similar to the corresponding triglyceride and mainly comprised the phosphatidylcholine and lysophosphatidylethanolamine. Furthermore, we synthesized the trioctylphophine oxide-coated CdSe-ZnS QDs (TOPO-CdSe-ZnS QDs) of different sizes by hot rapid injection including green-emitting QDs (2.67 nm), orange-emitting QDs (3.34 nm) and red-emitting QDs (4.48 nm) Before modification, the original TOPO-CdSe-ZnS QDs in hexane exhibited high quantum yield of 33.44% for green QDs, 24.9% for orange QDs and 33.36% for red QDs with the highest emission wavelength at 560, 578, and 628 nm, respectively. Then, the original QDs of different sizes were modified with camellia phospholipids through micelle formation. After modification with camellia phospholipids, the PLs-coated CdSe-ZnS QDs in 1 M Tris pH 10 exhibited the quantum yield of 2.84% for PLs-green QDs, 1.30% for PLs-orange QDs, and 2.43% for PLs-red QDs with the maximum emission peaks at 566 nm, 585 nm, and 631 nm, respectively. Moreover, The PLs-coated CdSe-ZnS QDs of three sizes in media retained the absorption spectra and unchanged in shapes, and the PLs-coated CdSe-ZnS QDs were dispersible and stable in aqueous media when compared with the TOPO-CdSe-ZnS QDs. For cytotoxicity test, the PLs-coated CdSe-ZnS QDs showed higher cell viability than the original TOPO-CdSe-ZnS QDs at high concentrations of 1mg/ml. For cytokine detection, the original TOPO-CdSe-ZnS QDs and the PLs-coated CdSe-ZnS QDs of three sizes induce the release in TNF-α and IL-6, but not IL-1β after 24 h incubation in 264.7 raw cell. From the results, we could use the byproducts from plant edible oil production such as phospholipids in the preparation of water-dispersible quantum dots, leading to reduction in toxic chemicals used and QDs with low toxicity in vitro. The obtained water-dispersible quantum dots were potentially useful for biological applications as alternative fluorescent markers and sensors.
DOI: 10.14457/cu.the.2015.410
title: Pseudomonas cepacia lipase immobilized onto chitosan-coated polystyrene beads for biodiesel production

description: In this research, chitosan-styrene copolymer coated onto polystyrene beads (CHI-STY/PS beads) was used as the solid support and the lipase of Pseudomonas cepacia (P. cepacia) was immobilized onto CHI-STY/PS beads by using glutaraldehyde as linker (GLU-CHI-STY/PS beads). Immobilization of lipase onto GLU-CHI-STY/PS beads was used as a catalyst for biodiesel production through transesterification reaction of soybean oil. For transesterification reaction, P. cepacia (30 mg) immobilized onto 15%GLU-2%CHI-15%STY/PS beads (1 g) at 30oC for 72 hours was used as a catalyst for biodiesel production. The lipase activity, protein loading yield and specific activity of this catalyst gave the optimal value at 54.43 U/g-support, 72.93% and 2.49 U/mg-protein, respectively. The effects of molar ratio of oil to alcohol, amount of catalyst, temperature and reaction time in the transesterification reaction were investigated. The optimum conditions were 1:5 oil/ethanol molar ratio (three step addition of anhydrous ethanol), 0.4294 g catalyst at 40°C for 32 hours. The highest ethyl esters yield was 91%. The ethyl esters production retained 87% of the original conversion after being reused for 5 batches.
DOI: 10.14457/cu.the.2015.411
title: Highly specific detection of biogenic amines using fluorescence-probe-modified silica nanoparticles

description: A goal of this research is to design and synthesize chemosensors for detection of catecholamine and histidine in water solution. To enhance water solubility, silica nanosphere (SNPs) and mesoporous silica nanoparticles (MSNs) were applied as a solid support which was modified by o-phthalic hemithioacetal (OPTA) to detect primary amine and give high fluorescence emission of isoindole product as a donor fluorophore in the detection system. Moreover, CB and ZnC2 were designed and synthesized as acceptor fluorophores to specifically detect catecholamine and histidine, respectively. Studies on the specific detection of SNPs-OPTA and MSNs-OPTA toward various biogenic amines in phosphate buffer solution, pH 7.4 were found that all biogenic amines enable to form an isoindole product which displayed the emission band at 450 nm (λex = 345nm). Interestingly, histidine showed the highest fluorescence intensity of isoindole product. According to studies on the factors which influence on the binding ability of this system, it was proposed that the imidazole in histidine played important role as a self-catalyst in isoindole product formation. To discriminate the catecholamine, CB was applied to detect various biogenic amines in SNPs-OPTA solution. Interestingly, only dopamine and norepinephrine showed a significant influence on the fluorescence quenching of coumarin at 550 nm. Therefore, this new fluorescence sensor system to discriminate the catecholamine under photoinduced electron transfer (PET) process has been successfully developed. For detection of histidine, the fluorescence self-quenching of isoindole product and ZnC2 was observed as a hypothesis of orderly packing of napthalimide based ZnC2 on the surface of silica (SNPs-OPTA). To avoid this effect, the system consisting of CTAB (SNPs-OPTA_CTAB) was applied. The fluorescence responses of isoindole in SNPs-OPTA_CTAB by varying amount of ZnC2 exhibited the promoting of energy transfer and the system showed high fluorescence intensity at 550 nm belonging to ZnC2 in case of histidine. It was clear that SNPs-OPTA and MSNs-OPTA offered the highly promising selectivity for histidine detection without the assistant of the acceptor fluorophore. However, the Fluorescence Resonance Energy Transfer (FRET) process is an expected benefit for higher wavelength of emission band to avoid the interference from biological matrix in sensing purpose.
DOI: 10.14457/cu.the.2015.412
title: Synthesis of resveratrol glycosides by cyclodextrin glycosyltransferase from Paenibacillus sp. RB01

description: In this work, resveratrol glycosides were synthesized by transglycosylation reaction from β-cyclodextrin (β-CD) donor to resveratrol catalyzed by cyclodextrin glycosyltransferase (CGTase) from Paenibacillus sp.RB01. The effect of DMSO on CGTase stability was determined. The result showed that the enzyme activities were dramatically decreased when DMSO concentration increased. β-cyclodextrin was chosen as an appropriate glycosyl donor and it was found that 20% (v/v) of DMSO gave the highest production yield. To investigate its synthesis ability, the reaction mixture containing 1.0% (w/v) of resveratrol in 20% (v/v) of DMSO and 1.0% (w/v) of β-cyclodextrin were incubated with 100 U/ml of CGTase at 40°C for 24 hours. Two spots of the expected products were observed at Rf of 0.55 and 0.77. The products were preliminary characterized by glucoamylase and α-glucosidase and it was concluded that the product was glycoside derivative and glucose molecules was attached to resveratrol with α-1,4-linkage. After optimization, the yield was increased by 3.71 times. The main product was collected by HPLC and its molecular mass and its possible structure were analyzed by MS and LC-MS/MS techniques, respectively. The molecular mass of the main product (resveratrol glycoside I, Rt~13.6 minutes ) was found to be 390 Daltons which corresponded to resveratrol monoglucoside having one monoglucosyl group attached to the position of 3-hydroxyl group of resveratrol. The water solubility of resveratrol glycoside I (Rt ~13.6 minutes) and II (Rt ~12.8 minutes) was about 72 and 26 times higher than that of resveratrol, respectively. While the DPPH• scavenging effect (%) of both of resveratrol glycoside I and II were lower than those of resveratrol and vitamin C.