DOI: 10.57760/sciencedb.24783
title: Gut metagenomic data of Coronaproctus castanopsis and two other coccid species in 2024
title: 2024年锥栗冠绵蚧及其他2种蚧虫肠道宏基因组数据

description: The samples of the cone chestnut crown scale and ball chain scale were collected from Qianjiangyuan National Park, and the Australian blowing scale was collected from Gannan Normal University. Sample its intestinal contents for testing.   Using Covaris focused ultrasound to break qualified DNA samples. After adding End Repair Mix and placing it at 20 ℃ for 30 minutes for end repair, the DNA fragment was purified using QIA quick PCR Purification Kit (Qiagen). Place A-Tailing Mix at 37 ℃ for 30 minutes and add A base at the 3 'end.       Connect sequencing adapters at both ends of the DNA fragment. 2% agarose gel and QIA quickGel Extraction kit (QIAGEN) were used for fragment selection and PCR amplification of several cycles.       The PCR product was purified by 2% agarose gel and the target fragment was recovered by QIA quick Gel Extraction kit (QIAGEN).       Use Agilent 2100 Bioanayzer and ABI StepOnePlus Real Time PCR System for quality control and quantification of the sample library. Sequencing of qualified libraries.   SampleID   TZC is an adult Chinese chestnut scale insect that feeds on sweet chestnut   TZY is a larva of the chestnut crown scale insect that feeds on sweet chestnut   MZC is an adult chestnut scale insect that feeds on rice chestnut   MZY is a larva of the chestnut scale insect that feeds on rice chestnut   MQY is a larva of the ball scale insect that feeds on rice chestnut   QZC is an adult chestnut scale insect that feeds on Qinggang   QCY is a larva of the Australian scale insect that feeds on navel oranges   QCC is an adult Australian scale insect that feeds on navel oranges 
description: The samples of the cone chestnut crown scale and ball chain scale were collected from Qianjiangyuan National Park, and the Australian blowing scale was collected from Gannan Normal University. Sample its intestinal contents for testing.   Using Covaris focused ultrasound to break qualified DNA samples. After adding End Repair Mix and placing it at 20 ℃ for 30 minutes for end repair, the DNA fragment was purified using QIA quick PCR Purification Kit (Qiagen). Place A-Tailing Mix at 37 ℃ for 30 minutes and add A base at the 3 'end.       Connect sequencing adapters at both ends of the DNA fragment. 2% agarose gel and QIA quickGel Extraction kit (QIAGEN) were used for fragment selection and PCR amplification of several cycles.       The PCR product was purified by 2% agarose gel and the target fragment was recovered by QIA quick Gel Extraction kit (QIAGEN).       Use Agilent 2100 Bioanayzer and ABI StepOnePlus Real Time PCR System for quality control and quantification of the sample library. Sequencing of qualified libraries.   SampleID   TZC is an adult Chinese chestnut scale insect that feeds on sweet chestnut   TZY is a larva of the chestnut crown scale insect that feeds on sweet chestnut   MZC is an adult chestnut scale insect that feeds on rice chestnut   MZY is a larva of the chestnut scale insect that feeds on rice chestnut   MQY is a larva of the ball scale insect that feeds on rice chestnut   QZC is an adult chestnut scale insect that feeds on Qinggang   QCY is a larva of the Australian scale insect that feeds on navel oranges   QCC is an adult Australian scale insect that feeds on navel oranges