cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30016895
title: Effect of soil and plant nutrients on starch yield of potato for processing under growers’ management in Hokkaido, Japan

description: The demand for potatoes for processing in Japan has been increasing due to a shift in consumption preference from table potatoes consumption to processing. However, the domestic production remains low due to a reduction in planted areas and stagnant yields. Soil nutrients and fertilization are crucial for achieving high yield and quality of potatoes for processing. Therefore, there is a need to develop proper potato nutrition management to meet the quality standards, especially starch yield, desired by processing industries. This study was carried out in Tokachi district, Hokkaido to examine how soil and plant nutrients affect the starch yield of potato for processing. It was conducted in two phases: (1) a field survey of 16 growers, where soil, shoot, and tuber samples were collected and analyzed, and (2) a field experiment testing two levels of N (60 and 120 kg ha<sup>−1</sup>) and Mg (0 and 40 kg ha<sup>−1</sup>). The growers’ fields varied in tuber and starch yields. Our findings indicate that higher soil available N decreases starch yield. In general, the soil available N in the growers’ fields was high to excessive, and shoot N concentrations were positively related to soil available N. Growth traits such as stem length, shoot biomass, NDVI, and SPAD values did not affect starch yield in the growers’ fields. The results also showed that among the nutrients and their molar ratios in the shoot, only the N/Mg ratio was significantly related to starch yield. Although no significant effects on starch yields were shown by the main factors (N and Mg), nor their interaction in the field experiment, tuber and starch yields trend increased with added magnesium, regardless of nitrogen levels, while higher nitrogen application rates promoted longer stems and greater shoot biomass. This study revealed that high N/Mg ratio were related with lower starch yields in farmers’ fields, which was supported by field experiment results where Mg was added tended to improve starch yield across N levels. Therefore, to increase starch yield in potato for processing should consider fertilization based on crop need by monitoring soil available nutrients to ensure a balanced nutrition.
DOI: 10.6084/m9.figshare.30016895.v1
title: Effect of soil and plant nutrients on starch yield of potato for processing under growers’ management in Hokkaido, Japan

description: The demand for potatoes for processing in Japan has been increasing due to a shift in consumption preference from table potatoes consumption to processing. However, the domestic production remains low due to a reduction in planted areas and stagnant yields. Soil nutrients and fertilization are crucial for achieving high yield and quality of potatoes for processing. Therefore, there is a need to develop proper potato nutrition management to meet the quality standards, especially starch yield, desired by processing industries. This study was carried out in Tokachi district, Hokkaido to examine how soil and plant nutrients affect the starch yield of potato for processing. It was conducted in two phases: (1) a field survey of 16 growers, where soil, shoot, and tuber samples were collected and analyzed, and (2) a field experiment testing two levels of N (60 and 120 kg ha<sup>−1</sup>) and Mg (0 and 40 kg ha<sup>−1</sup>). The growers’ fields varied in tuber and starch yields. Our findings indicate that higher soil available N decreases starch yield. In general, the soil available N in the growers’ fields was high to excessive, and shoot N concentrations were positively related to soil available N. Growth traits such as stem length, shoot biomass, NDVI, and SPAD values did not affect starch yield in the growers’ fields. The results also showed that among the nutrients and their molar ratios in the shoot, only the N/Mg ratio was significantly related to starch yield. Although no significant effects on starch yields were shown by the main factors (N and Mg), nor their interaction in the field experiment, tuber and starch yields trend increased with added magnesium, regardless of nitrogen levels, while higher nitrogen application rates promoted longer stems and greater shoot biomass. This study revealed that high N/Mg ratio were related with lower starch yields in farmers’ fields, which was supported by field experiment results where Mg was added tended to improve starch yield across N levels. Therefore, to increase starch yield in potato for processing should consider fertilization based on crop need by monitoring soil available nutrients to ensure a balanced nutrition.
DOI: 10.13130/rd_unimi/6zytln/vyzoyt
title: MEDWATERICE DMP_CS2_SDI_Cobert_2020.xlsx
DOI: 10.13130/rd_unimi/6zytln/x4kr0a
title: MEDWATERICE DMP_CS2_Mas Pla_Wet sowing_2020.xlsx
DOI: 10.6084/m9.figshare.29414041
title: Impact of effective soil depth on agronomic recommendations for sorghum and its economic viability in Africa

description: Improving sorghum productivity, the second most important upland crop in Africa, is critical to meeting the continent’s rapidly growing food demand. Previous research indicated that limited soil water, especially in soils with an effective soil depth (ESD) of ≤ 50 cm, restricts sorghum production under fertilized conditions. This finding suggests that optimal management practices, including fertilizer application rates, planting density, and variety selection, should vary by ESD. However, agronomic recommendations for sorghum in Africa have yet to incorporate ESD considerations. This study assessed the impact of ESD on optimal sorghum management practices and their economic stability amid fluctuating fertilizer prices. Two-year field experiments were conducted on soils with ESDs of 25, 50, and 100 cm in the Sudan Savanna, Africa’s largest sorghum-producing region. These ESDs represent three dominant soil types in the region: two types of Plinthosols and Ferric Lixisol. Results showed that the optimal management practices varied by ESD: 37 kg N ha<sup>−1</sup>, 3.1 hills m<sup>−2</sup>, and Kapelga for 25 cm ESD; 74 kg N ha<sup>−1</sup>, 8.3 hills m<sup>−2</sup>, and Kapelga for 50 cm ESD; and 74 kg N ha<sup>−1</sup>, 5.6 hills m<sup>−2</sup>, and Sariaso14 for 100 cm ESD. These practices were economically robust for ESDs of 50 and 100 cm but not for 25 cm. A random forest regression model identified ESD as the most critical factor for sorghum yield, surpassing rainfall, nitrogen application rate, planting density, and variety. This study highlights the need for ESD-based agronomic recommendations to enhance food security and income stability for African farmers.
DOI: 10.6084/m9.figshare.29414041.v1
title: Impact of effective soil depth on agronomic recommendations for sorghum and its economic viability in Africa

description: Improving sorghum productivity, the second most important upland crop in Africa, is critical to meeting the continent’s rapidly growing food demand. Previous research indicated that limited soil water, especially in soils with an effective soil depth (ESD) of ≤ 50 cm, restricts sorghum production under fertilized conditions. This finding suggests that optimal management practices, including fertilizer application rates, planting density, and variety selection, should vary by ESD. However, agronomic recommendations for sorghum in Africa have yet to incorporate ESD considerations. This study assessed the impact of ESD on optimal sorghum management practices and their economic stability amid fluctuating fertilizer prices. Two-year field experiments were conducted on soils with ESDs of 25, 50, and 100 cm in the Sudan Savanna, Africa’s largest sorghum-producing region. These ESDs represent three dominant soil types in the region: two types of Plinthosols and Ferric Lixisol. Results showed that the optimal management practices varied by ESD: 37 kg N ha<sup>−1</sup>, 3.1 hills m<sup>−2</sup>, and Kapelga for 25 cm ESD; 74 kg N ha<sup>−1</sup>, 8.3 hills m<sup>−2</sup>, and Kapelga for 50 cm ESD; and 74 kg N ha<sup>−1</sup>, 5.6 hills m<sup>−2</sup>, and Sariaso14 for 100 cm ESD. These practices were economically robust for ESDs of 50 and 100 cm but not for 25 cm. A random forest regression model identified ESD as the most critical factor for sorghum yield, surpassing rainfall, nitrogen application rate, planting density, and variety. This study highlights the need for ESD-based agronomic recommendations to enhance food security and income stability for African farmers.
DOI: 10.13130/rd_unimi/6zytln/t95h4w
title: MEDWATERICE DMP_CS2_SDI_Benzinera_2020.xlsx
DOI: 10.6084/m9.figshare.29432363
title: Impact of methane fermentation waste fluid application rates on maize yield and fate of N with special reference to soil textures

description: Efficient nitrogen (N) utilization is crucial for improving fertilizer use efficiency in crop production. Methane fermentation waste fluid (MFW) has emerged as a promising biofertilizer; however, its interaction with soil texture and application rates remains inadequately understood. This study aimed to evaluate the effects of MFW application rates on maize (Zea mays L.) growth and N dynamics under contrasting soil textures – sandy loam (SL) and clayey loam (CL) – and to identify the optimum application rates for balancing crop productivity and environmental impacts. Pot experiments (1/2000a) were conducted over two years with three MFW application rates (375, 750, and 1500 mg N pot<sup>−1</sup>), a chemical fertilizer (NH<sub>4</sub>Cl, 750 mg N pot<sup>−1</sup>), and a control treatment (0N). To track N derived from inorganic fertilizer (Ndfif) across plant uptake, soil retention, and environmental losses, <sup>15</sup>NH<sub>4</sub>Cl (99%) was added as a tracer while keeping the total N content in each treatment. The results indicated that maize SPAD values, biomass, and yield were significantly enhanced in SL soil than in CL soil. Increasing MFW rates improved maize yield in 2021 but showed reduced effectiveness under limited irrigation in 2022. Despite lower Ndfif retention in SL soil, total Ndfif recovery (soil and plant) was significantly higher (73–79%) compared to CL soil (53–76%), which was strongly influenced by MFW rates and water availability. Ndfif losses were more pronounced in CL soil due to NH<sub>3</sub> volatilization and NO<sub>3</sub>-N leaching, especially at higher MFW rates. Although N recovery from MFW was lower than chemical fertilizer at equivalent N levels, MFW provided sufficient N for maize yield. Considering yield and environmental impact, the optimum MFW application rate was estimated at 700–1000 mg N pot<sup>−1</sup> for SL soil and 500–776 mg N pot<sup>−1</sup> for CL soil. Our findings highlight that while MFW is highly effective in sandy soils, its application in clayey soils requires careful management to mitigate N losses through volatilization and leaching. By tailoring MFW rates to soil texture and water regimes, it is possible to enhance maize productivity and reduce environmental impacts, supporting sustainable nutrient management strategies in agricultural systems.
DOI: 10.6084/m9.figshare.30120461
title: Long-term changes in macronutrients, micronutrients, and potentially toxic elements under 33 years of continuous chemical fertilizer and organic matter application

description: This study examined the effects of chemical fertilizer and organic material [rice straw (RS) and RS compost] applications over 33 years (began in 1982) in a rice paddy on soil properties and nutrients. From 1995 to 2013, soil samples from the 0–12 cm layers were collected every 3 years from five fertilizer treatments: (1) PK chemical fertilizer only, (2) NPK fertilizer (NPK), (3) NPK + RS, (4) NPK + RS compost, and (5) NPK + high-dose RS compost. This analysis assessed changes in soil macro- (C, N, Ca, Mg, K, and P) and micronutrient elements (Fe, Mn, Zn, Ni, Cu, and Mo) and other potentially toxic elements (Al, Na, As, Cr, and Pb). Results revealed that organic matter additions increased the content of C and N over the soil sampling period. RS and RS compost treatments enhanced the annual SOC increase (0.089 − 0.477 g kg<sup>−1</sup> year<sup>−1</sup>) compared with N treatment alone (0.010 − 0.039 g kg<sup>−1</sup> year<sup>−1</sup>), suggesting that composting markedly improved N uptake and soil microbial activity. The content of macronutrient elements (Ca, Mg, P, and K) remained stable with organic matter added treatments, with Ca and P significantly correlating with C and N (<i>p</i> &lt; 0.05). However, micronutrient element levels of Fe, Mn, and Zn decreased, likely due to redox reactions and runoff; meanwhile, potentially toxic elements showed weaker association with organic matter addition and different accumulation patterns in the soil. Principal component analysis confirmed that C, N, P, and Ca exhibited strong interrelationships and were closely associated with compost addition. Overall, long-term applications of organic matter in single-crop paddy rice systems in Japan tended to enhance the accumulation of macronutrient elements.
DOI: 10.6084/m9.figshare.30120461.v1
title: Long-term changes in macronutrients, micronutrients, and potentially toxic elements under 33 years of continuous chemical fertilizer and organic matter application

description: This study examined the effects of chemical fertilizer and organic material [rice straw (RS) and RS compost] applications over 33 years (began in 1982) in a rice paddy on soil properties and nutrients. From 1995 to 2013, soil samples from the 0–12 cm layers were collected every 3 years from five fertilizer treatments: (1) PK chemical fertilizer only, (2) NPK fertilizer (NPK), (3) NPK + RS, (4) NPK + RS compost, and (5) NPK + high-dose RS compost. This analysis assessed changes in soil macro- (C, N, Ca, Mg, K, and P) and micronutrient elements (Fe, Mn, Zn, Ni, Cu, and Mo) and other potentially toxic elements (Al, Na, As, Cr, and Pb). Results revealed that organic matter additions increased the content of C and N over the soil sampling period. RS and RS compost treatments enhanced the annual SOC increase (0.089 − 0.477 g kg<sup>−1</sup> year<sup>−1</sup>) compared with N treatment alone (0.010 − 0.039 g kg<sup>−1</sup> year<sup>−1</sup>), suggesting that composting markedly improved N uptake and soil microbial activity. The content of macronutrient elements (Ca, Mg, P, and K) remained stable with organic matter added treatments, with Ca and P significantly correlating with C and N (<i>p</i> &lt; 0.05). However, micronutrient element levels of Fe, Mn, and Zn decreased, likely due to redox reactions and runoff; meanwhile, potentially toxic elements showed weaker association with organic matter addition and different accumulation patterns in the soil. Principal component analysis confirmed that C, N, P, and Ca exhibited strong interrelationships and were closely associated with compost addition. Overall, long-term applications of organic matter in single-crop paddy rice systems in Japan tended to enhance the accumulation of macronutrient elements.
DOI: 10.13130/rd_unimi/6zytln/j19qhy
title: README FILE_CS2_Baix Ter_Spain_2020.txt
DOI: 10.6084/m9.figshare.29432363.v1
title: Impact of methane fermentation waste fluid application rates on maize yield and fate of N with special reference to soil textures

description: Efficient nitrogen (N) utilization is crucial for improving fertilizer use efficiency in crop production. Methane fermentation waste fluid (MFW) has emerged as a promising biofertilizer; however, its interaction with soil texture and application rates remains inadequately understood. This study aimed to evaluate the effects of MFW application rates on maize (Zea mays L.) growth and N dynamics under contrasting soil textures – sandy loam (SL) and clayey loam (CL) – and to identify the optimum application rates for balancing crop productivity and environmental impacts. Pot experiments (1/2000a) were conducted over two years with three MFW application rates (375, 750, and 1500 mg N pot<sup>−1</sup>), a chemical fertilizer (NH<sub>4</sub>Cl, 750 mg N pot<sup>−1</sup>), and a control treatment (0N). To track N derived from inorganic fertilizer (Ndfif) across plant uptake, soil retention, and environmental losses, <sup>15</sup>NH<sub>4</sub>Cl (99%) was added as a tracer while keeping the total N content in each treatment. The results indicated that maize SPAD values, biomass, and yield were significantly enhanced in SL soil than in CL soil. Increasing MFW rates improved maize yield in 2021 but showed reduced effectiveness under limited irrigation in 2022. Despite lower Ndfif retention in SL soil, total Ndfif recovery (soil and plant) was significantly higher (73–79%) compared to CL soil (53–76%), which was strongly influenced by MFW rates and water availability. Ndfif losses were more pronounced in CL soil due to NH<sub>3</sub> volatilization and NO<sub>3</sub>-N leaching, especially at higher MFW rates. Although N recovery from MFW was lower than chemical fertilizer at equivalent N levels, MFW provided sufficient N for maize yield. Considering yield and environmental impact, the optimum MFW application rate was estimated at 700–1000 mg N pot<sup>−1</sup> for SL soil and 500–776 mg N pot<sup>−1</sup> for CL soil. Our findings highlight that while MFW is highly effective in sandy soils, its application in clayey soils requires careful management to mitigate N losses through volatilization and leaching. By tailoring MFW rates to soil texture and water regimes, it is possible to enhance maize productivity and reduce environmental impacts, supporting sustainable nutrient management strategies in agricultural systems.
DOI: 10.6084/m9.figshare.29432363.v2
title: Impact of methane fermentation waste fluid application rates on maize yield and fate of N with special reference to soil textures

description: Efficient nitrogen (N) utilization is crucial for improving fertilizer use efficiency in crop production. Methane fermentation waste fluid (MFW) has emerged as a promising biofertilizer; however, its interaction with soil texture and application rates remains inadequately understood. This study aimed to evaluate the effects of MFW application rates on maize (Zea mays L.) growth and N dynamics under contrasting soil textures – sandy loam (SL) and clayey loam (CL) – and to identify the optimum application rates for balancing crop productivity and environmental impacts. Pot experiments (1/2000a) were conducted over two years with three MFW application rates (375, 750, and 1500 mg N pot<sup>−1</sup>), a chemical fertilizer (NH<sub>4</sub>Cl, 750 mg N pot<sup>−1</sup>), and a control treatment (0N). To track N derived from inorganic fertilizer (Ndfif) across plant uptake, soil retention, and environmental losses, <sup>15</sup>NH<sub>4</sub>Cl (99%) was added as a tracer while keeping the total N content in each treatment. The results indicated that maize SPAD values, biomass, and yield were significantly enhanced in SL soil than in CL soil. Increasing MFW rates improved maize yield in 2021 but showed reduced effectiveness under limited irrigation in 2022. Despite lower Ndfif retention in SL soil, total Ndfif recovery (soil and plant) was significantly higher (73–79%) compared to CL soil (53–76%), which was strongly influenced by MFW rates and water availability. Ndfif losses were more pronounced in CL soil due to NH<sub>3</sub> volatilization and NO<sub>3</sub>-N leaching, especially at higher MFW rates. Although N recovery from MFW was lower than chemical fertilizer at equivalent N levels, MFW provided sufficient N for maize yield. Considering yield and environmental impact, the optimum MFW application rate was estimated at 700–1000 mg N pot<sup>−1</sup> for SL soil and 500–776 mg N pot<sup>−1</sup> for CL soil. Our findings highlight that while MFW is highly effective in sandy soils, its application in clayey soils requires careful management to mitigate N losses through volatilization and leaching. By tailoring MFW rates to soil texture and water regimes, it is possible to enhance maize productivity and reduce environmental impacts, supporting sustainable nutrient management strategies in agricultural systems.
DOI: 10.6084/m9.figshare.30272057
title: Induction of uncharacterized <i>NRT2</i> genes by high-pH stress in barley

description: In plants, nutrient uptake and root elongation typically depend on acidic conditions in the root apoplast. High pH impairs these processes, posing a significant challenge for crop growth in alkaline soils. Barley is widely recognized for its alkaline tolerance. We previously demonstrated that it maintains root elongation under high pH conditions, in contrast to many other plant species. This suggests that barley possesses an unknown mechanism enabling root growth under alkaline stress. In this study, we conducted transcriptome analysis of the root tips, including the apical meristem, of barley cultivar Ehimehadaka No. 1 exposed to high pH. We identified rapid and sustained induction of high-affinity nitrate transporter (<i>NRT2</i>) genes in response to high pH. The cultivar possesses at least 10 <i>NRT2</i> genes, and three genes from a previously uncharacterized phylogenetic clade showed rapid and consistent upregulation in both the root tip and upper root regions. These <i>NRT2</i> genes were downregulated under nitrogen-deficient conditions, suggesting that their high pH-induced expression is not simply a result of reduced nitrate uptake. Notably, nitrate uptake was initially suppressed under high pH but rapidly recovers and was maintained at pH 8, indicating that this cultivar can sustain nitrate acquisition even under proton-deficient conditions. Our findings suggest that <i>NRT2</i> gene induction may contribute to barley’s ability to adapt to high pH environments.
DOI: 10.6084/m9.figshare.30272057.v1
title: Induction of uncharacterized <i>NRT2</i> genes by high-pH stress in barley

description: In plants, nutrient uptake and root elongation typically depend on acidic conditions in the root apoplast. High pH impairs these processes, posing a significant challenge for crop growth in alkaline soils. Barley is widely recognized for its alkaline tolerance. We previously demonstrated that it maintains root elongation under high pH conditions, in contrast to many other plant species. This suggests that barley possesses an unknown mechanism enabling root growth under alkaline stress. In this study, we conducted transcriptome analysis of the root tips, including the apical meristem, of barley cultivar Ehimehadaka No. 1 exposed to high pH. We identified rapid and sustained induction of high-affinity nitrate transporter (<i>NRT2</i>) genes in response to high pH. The cultivar possesses at least 10 <i>NRT2</i> genes, and three genes from a previously uncharacterized phylogenetic clade showed rapid and consistent upregulation in both the root tip and upper root regions. These <i>NRT2</i> genes were downregulated under nitrogen-deficient conditions, suggesting that their high pH-induced expression is not simply a result of reduced nitrate uptake. Notably, nitrate uptake was initially suppressed under high pH but rapidly recovers and was maintained at pH 8, indicating that this cultivar can sustain nitrate acquisition even under proton-deficient conditions. Our findings suggest that <i>NRT2</i> gene induction may contribute to barley’s ability to adapt to high pH environments.
DOI: 10.13130/rd_unimi/kfiayf/k8iycx
title: S1C Drp1 40sec HS.tif
DOI: 10.13130/rd_unimi/kfiayf/t2iaxh
title: S1C blot not boil 2sec.tif
DOI: 10.13130/rd_unimi/kfiayf/rukusu
title: S1C analisi globale 1m+5m (1).pzfx
DOI: 10.13130/rd_unimi/kfiayf/nvp9w8
title: S1C CIII 4sec HR OK.tif
DOI: 10.13130/rd_unimi/kfiayf/ok1ktb
title: S1C blot not boil 2sec-1 1m.jpg
DOI: 10.13130/rd_unimi/kfiayf/jco5l0
title: S1D-E Biog NEW MG new analisi.pzf
DOI: 10.13130/rd_unimi/kfiayf/sklfi5
title: S1C drp1-CI-CIII-mtCOI-CIV 2sec HR-1 cox 5m.jpg
DOI: 10.13130/rd_unimi/kfiayf/cdofjm
title: S1C drp1-CI-CIII-mtCOI-CIV 2sec HR.tif
DOI: 10.13130/rd_unimi/kfiayf/e48fw4
title: S1C drp1-CI-CIII-mtCOI-CIV 2sec HR-1.jpg
DOI: 10.13130/rd_unimi/kfiayf/q5txlv
title: S1B TEM analysis.pzf