cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/97cv0-akm52
title: MS411-R1-16 / WM1652

description: to capture F-piligrid box: YWC095
description: <strong>Tilt Series Date:</strong> 2014-04-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strains from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MS411-R1-16 & WM1652 grew to OD 0.6 in LB. Mix 2.5 ul of each onto grids on LB-agar plate, incubate 30 min for conjugation.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 22.5 C, 3µl BSA treated 10 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 1 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-04-07-4_slicer6974.jpg: Peptidoglycan ycw2014-04-07-4_slicer6978.jpg: filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-4/rawdata/</p>20140407_YWC095_20003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-4/rawdata/20140407_YWC095_20003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140407_YWC095_20003_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-4/3dimage_41128/20140407_YWC095_20003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jn6qg-7pw37
title: Vibrio cholerae MKW1383

description: Vibrio cholerae MKW1383 in the mixture with Bdellovibrio.Imaged by Janelia farm Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
  ycw2013-05-01-30_slicer9837.jpg: Vibrio cholerae .  cytoplasmic chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-30/rawdata/</p>grid3-tiltseries0031.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-30/rawdata/grid3-tiltseries0031.mrc"
        > <i class="download icon"></i> Download </a></p>

        grid3-tiltseries0031_part131_3.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-30/3dimage_26428/grid3-tiltseries0031_part131_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.28143197
title: Cholesteric elastomer waveguides doped with metallic nanospheres: exhibiting metamaterial-like behaviour under transverse elongation

description: The propagation of electromagnetic waves through a cholesteric elastomer slab doped with a small amount of randomly distributed metallic nanospheres, which is subjected to transverse elongation, is considered. The nanocomposite dielectric response formed by the cholesteric and the nanospheres is described using a Maxwell Garnett formalism. The real and imaginary parts of the dispersion relation are calculated numerically by solving Maxwell’s equations, subjected to the corresponding boundary conditions. The presence of two peaks in the imaginary part of the wave number of the hybrid system, caused by the plasmonic resonance of the metal, is observed. The group velocity versus frequency is also calculated, revealing two intervals where this quantity opposes the wave vector, giving rise to behaviour similar to that of a metamaterial waveguide. The behaviour is confirmed by the calculation of the Poynting vector, where the energy travels in the opposite direction to the wave vector.
DOI: 10.6084/m9.figshare.28143197.v1
title: Cholesteric elastomer waveguides doped with metallic nanospheres: exhibiting metamaterial-like behaviour under transverse elongation

description: The propagation of electromagnetic waves through a cholesteric elastomer slab doped with a small amount of randomly distributed metallic nanospheres, which is subjected to transverse elongation, is considered. The nanocomposite dielectric response formed by the cholesteric and the nanospheres is described using a Maxwell Garnett formalism. The real and imaginary parts of the dispersion relation are calculated numerically by solving Maxwell’s equations, subjected to the corresponding boundary conditions. The presence of two peaks in the imaginary part of the wave number of the hybrid system, caused by the plasmonic resonance of the metal, is observed. The group velocity versus frequency is also calculated, revealing two intervals where this quantity opposes the wave vector, giving rise to behaviour similar to that of a metamaterial waveguide. The behaviour is confirmed by the calculation of the Poynting vector, where the energy travels in the opposite direction to the wave vector.
DOI: 10.22002/8hvct-d5935
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC065
description: <strong>Tilt Series Date:</strong> 2014-05-12</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2014-05-12-21_slicer7299.jpg: T6SS
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-21/3dimage_42418/</p>20140512_YWC065_40002_part121_19.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-21/3dimage_42418/20140512_YWC065_40002_part121_19.rec"
        > <i class="download icon"></i> Download </a></p>

        20140512_YWC065_40002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-12-21/rawdata/20140512_YWC065_40002.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.28009016.v1
title: Marangoni flow across different phases in cyanobiphenyl liquid crystals under temperature gradient

description: Marangoni flows driven by temperature gradient in two-phase coexisting states are investigated in this study, using several liquid crystals of the cyanobiphenyl (CB) series. The flow field is measured using the fluorescence photo-bleaching method in sandwich cells, and the characteristic flow is observed near the air interface in all samples. The flow direction is from the isotropic phase to the nematic phase in 5CB and 6CB and from the nematic to the smectic phase in 8CB, which is the case for the three different coating materials of the cell substrates. However, the flow direction observed in 7CB and 8CB changes depending on the coating material in the coexisting state of the nematic and isotropic phases. These results can be explained by the temperature dependence of surface tension γ and the air-interface shape ls near phase-transition temperatures.
DOI: 10.22002/xxm9y-fbw72
title: Bdellovibrio/Vibrio cholerae

description: Bdellovibrio invasion complex with Vibrio cholerae MKW1383.Imaged by Janelia farm Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-26/rawdata/</p>grid3-tiltseries0026.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-26/rawdata/grid3-tiltseries0026.mrc"
        > <i class="download icon"></i> Download </a></p>

        grid3-tiltseries0026_full.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-26/3dimage_26421/grid3-tiltseries0026_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ebd5v-bv520
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-25-64_slicer10160.jpg: Helicobacter pylori .  flat vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-64/rawdata/</p>20131025_Janelia_YWC043_20011.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-64/rawdata/20131025_Janelia_YWC043_20011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_20011_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-64/3dimage_33537/20131025_Janelia_YWC043_20011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16990839
title: FIShBOT Archive 2025-08-29

description: This dataset is a static snapshot of the FIShBOT data product before new data are aggregated. This version of fishbot is archived for reproducibility and is not updated. The data are aggregated from the FIShBOT system, which collects and processes oceanographic from fishery dependent and independent sources.
DOI: 10.5281/zenodo.16990840
title: FIShBOT Archive 2025-08-29

description: This dataset is a static snapshot of the FIShBOT data product before new data are aggregated. This version of fishbot is archived for reproducibility and is not updated. The data are aggregated from the FIShBOT system, which collects and processes oceanographic from fishery dependent and independent sources.
DOI: 10.22002/xzqrx-w3z64
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection24 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-01-07</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.4. At 24 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-07-13/rawdata/</p>stiv24h0002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-07-13/rawdata/stiv24h0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        stiv24h0002_part111_56.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-07-13/3dimage_37517/stiv24h0002_part111_56.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8t58z-96a97
title: H.pylori 26695 WT

description: Grid box YWC089.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-01-29_slicer9183.jpg: Helicobacter pylori .  filament ycw2014-03-01-29_slicer9184.jpg: Helicobacter pylori .  chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-29/rawdata/</p>20140301_YWC089_20021.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-29/rawdata/20140301_YWC089_20021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140301_YWC089_20021_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-29/3dimage_39460/20140301_YWC089_20021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zqchq-z9m17
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter lysed n the presence of the crowding agent 10% Polyvinylpyrrolidone (PVP)
description: <strong>Tilt Series Date:</strong> 2013-10-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Protocol:-     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xg-     resuspended PVP in 80µL appropriate Buffer 2Buffer 1:Crowding agent1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0Buffer 2:Crowding agentQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-16/rawdata/</p>Rhodopvp27k0018.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-16/rawdata/Rhodopvp27k0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp27k0018SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-16/3dimage_46404/Rhodopvp27k0018SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/am025-qy515
title: Myxococcus xanthus delta-pilB

description: grid box YWC066
description: <strong>Tilt Series Date:</strong> 2013-12-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2013-12-31-40_slicer7767.jpg: nested vesicle .  extracellular vesicles .  Myxococcus Xanthus ycw2013-12-31-40_slicer7768.jpg: nested vesicle .  extracellular vesicles .  Myxococcus Xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-31-40/rawdata/</p>20131231_YWC066_10040.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-31-40/rawdata/20131231_YWC066_10040.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131231_YWC066_10040_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-31-40/3dimage_37006/20131231_YWC066_10040_full.rec"
        > <i class="download icon"></i> Download </a></p>