cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8195030.v1
title: Small cell bladder cancer: should we consider prophylactic cranial irradiation?

description: ABSTRACT  Purpose: To describe the clinical characteristics, treatment patterns, and outcomes in patients with small cell bladder cancer at our institution, including those who received prophylactic cranial irradiation (PCI) for the prevention of intracranial recurrence.  Materials and Methods: Patients with small cell bladder cancer treated at a single institution between January 1990 and August 2015 were identified and analyzed retrospectively for demographics, tumor stage, treatment, and overall survival.  Results: Of 44 patients diagnosed with small cell bladder cancer, 11 (25%) had metastatic disease at the time of presentation. Treatment included systemic chemotherapy (70%), radical surgery (59%), and local radiation (39%). Six patients (14%) received PCI. Median overall survival was 10 months (IQR 4 – 41). Patients with extensive disease had worse overall survival than those with organ confined disease (8 months vs. 36 months, respectively, p = 0.04). Among those who received PCI, 33% achieved 5 - year survival.  Conclusion: Outcomes for patients with small cell bladder cancer remain poor. Further research is indicated to determine if PCI increases overall survival in small call bladder cancer patients, especially those with extensive disease who respond to chemotherapy.
DOI: 10.6084/m9.figshare.8195033
title: High expectations. Interregional agendas on global security challenges: East Asia, Europe and Latin America

description: Abstract Interregionalism has been increasingly used to advance cooperation on regional and global security challenges. This study examines three interregional dialogues comprising East Asia, Europe, and Latin America. Each interregional security agenda reflects specific concerns and different evolving paths. Insights from ‘multilateral security governance’ approaches can reinforce the analysis of how security agendas emerge and change, and how their related norms and practices evolve.
DOI: 10.6084/m9.figshare.8195033.v1
title: High expectations. Interregional agendas on global security challenges: East Asia, Europe and Latin America

description: Abstract Interregionalism has been increasingly used to advance cooperation on regional and global security challenges. This study examines three interregional dialogues comprising East Asia, Europe, and Latin America. Each interregional security agenda reflects specific concerns and different evolving paths. Insights from ‘multilateral security governance’ approaches can reinforce the analysis of how security agendas emerge and change, and how their related norms and practices evolve.
DOI: 10.6084/m9.figshare.8195036
title: Adiposity indicators as a screening method for polysomnography in shift workers

description: Abstract  Objective: to verify the discriminatory power of adiposity indicators in the prediction of obstructive sleep apnoea (OSA) in shift workers.  Methods:  a cross-sectional study carried out in an iron ore extraction company, in Minas Gerais, Brazil. Anthropometric data were collected and polysomnography (PSG) was performed in 118 male shift workers who owned at least one overall risk factor for cardiovascular disease.  Results:  the OSA prevalence in the sample was 84.7%. Among the adiposity indicators used to predict OSA (≥ 5 events/hour), body mass index (BMI), waist circumference (WC), waist-to-height ratio (WHtR), and total body fat (TBF) showed sensitivity values higher than 70%. Visceral fat (VF), neck circumference (NC), and neck-to-height ratio (NHtR) were the most effective in correctly identifying workers without OSA (specificity values higher than 70%). The areas under the receiver operating characteristic (ROC) curves for WC and NHtR were greater than 0.7, which indicated the test was effective in discriminating individuals with OSA.  Conclusions:  alterations in abdomen and neck adiposity indicators have a significant relationship with the presence of OSA and showed effectiveness as a screening method for PSG. WC and NHtR are considered good indicators for OSA prediction.
DOI: 10.6084/m9.figshare.8195042
title: Determination of Phenolic Compounds in Red Sweet Pepper (Capsicum annuum L.) Using a Modified QuEChERS Method and UHPLC-MS/MS Analysis and Its Relation to Antioxidant Activity

description: In the present work, a fast, easy and efficient analytical method was developed and validated for the determination of phenolic compounds (4-hydroxybenzoic, vanillic, caffeic, p-coumaric, sinapic, ferulic and ellagic acids, and naringenin) in red sweet pepper. Extraction of phenolic compounds was carried out using the quick, easy, cheap, effective, rugged and safe (QuEChERS) method, followed by separation and detection using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). When the acetate version of the QuEChERS method was used, higher amounts of the phenolic compounds were extracted. In the dispersive solid phase extraction (d-SPE) clean-up step, combination of 50 mg of octadecylsilane (C18) and 7.5 mg of graphitized carbon black (GCB), resulted in the greatest removal of interferents, especially carotenoids, without significant retention of phenolic compounds. The recoveries for the proposed method were 82-103%, the limits of quantification were 2-150 µg kg-1, and the precision values expressed in terms of relative standard deviation (RSD) were ≤ 15%. The method developed was successfully applied to the analysis of different red sweet pepper cultivars.
DOI: 10.6084/m9.figshare.8195042.v1
title: Determination of Phenolic Compounds in Red Sweet Pepper (Capsicum annuum L.) Using a Modified QuEChERS Method and UHPLC-MS/MS Analysis and Its Relation to Antioxidant Activity

description: In the present work, a fast, easy and efficient analytical method was developed and validated for the determination of phenolic compounds (4-hydroxybenzoic, vanillic, caffeic, p-coumaric, sinapic, ferulic and ellagic acids, and naringenin) in red sweet pepper. Extraction of phenolic compounds was carried out using the quick, easy, cheap, effective, rugged and safe (QuEChERS) method, followed by separation and detection using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). When the acetate version of the QuEChERS method was used, higher amounts of the phenolic compounds were extracted. In the dispersive solid phase extraction (d-SPE) clean-up step, combination of 50 mg of octadecylsilane (C18) and 7.5 mg of graphitized carbon black (GCB), resulted in the greatest removal of interferents, especially carotenoids, without significant retention of phenolic compounds. The recoveries for the proposed method were 82-103%, the limits of quantification were 2-150 µg kg-1, and the precision values expressed in terms of relative standard deviation (RSD) were ≤ 15%. The method developed was successfully applied to the analysis of different red sweet pepper cultivars.
DOI: 10.6084/m9.figshare.8195045
title: Peach palm flour (Bactris gasipae KUNTH): potential application in the food industry

description: Abstract Peach palm flours prepared from the landraces microcarpa (MIF), mesocarpa (MEF), and macrocarpa (MAF) were assessed for their functional and morphological characteristics. The flours had moisture contents in accordance with the current legislation, considerable lipid (≥3.95%) and carbohydrate (≥66.68%) contents, and potential as an energy source (≥300 kcal/100 g). MEF and MAF can be considered rich in dietary fibers since their fiber contents were above 6 g/100 g, which contributes to the nutritional enrichment of products prepared with those flours. The assessment of the technological properties, WAC (≥255%) and OAC (≥65%) showed the peach palm flours can be used as raw material in different products such as breads, cakes, meat products, and breaded foods, thus adding energy, nutritional, and economic value. It is worth pointing out that peach palm is gluten free, hence it is an option for the development of new products targeted at consumers with celiac disease.
DOI: 10.6084/m9.figshare.8195045.v1
title: Peach palm flour (Bactris gasipae KUNTH): potential application in the food industry

description: Abstract Peach palm flours prepared from the landraces microcarpa (MIF), mesocarpa (MEF), and macrocarpa (MAF) were assessed for their functional and morphological characteristics. The flours had moisture contents in accordance with the current legislation, considerable lipid (≥3.95%) and carbohydrate (≥66.68%) contents, and potential as an energy source (≥300 kcal/100 g). MEF and MAF can be considered rich in dietary fibers since their fiber contents were above 6 g/100 g, which contributes to the nutritional enrichment of products prepared with those flours. The assessment of the technological properties, WAC (≥255%) and OAC (≥65%) showed the peach palm flours can be used as raw material in different products such as breads, cakes, meat products, and breaded foods, thus adding energy, nutritional, and economic value. It is worth pointing out that peach palm is gluten free, hence it is an option for the development of new products targeted at consumers with celiac disease.
DOI: 10.6084/m9.figshare.8195048
title: Cardiac function and intracellular Ca2+ handling proteins are not impaired by high-saturated-fat diet-induced obesity

description: Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this study was to test the hypothesis that this dietary model of obesity leads to cardiac dysfunction resulting from alterations in the regulatory proteins of intracellular Ca2+ homeostasis. Male Wistar rats were distributed into two groups: control (C, n=18; standard diet) and obese (Ob, n=19; high-saturated-fat diet), which were fed for 33 weeks. Cardiac structure and function were evaluated using echocardiographic and isolated papillary muscle analyses. Myocardial protein expressions of sarcoplasmic reticulum Ca2+-ATPase, phospholamban (PLB), PLB serine-16 phosphorylation, PLB threonine-17 phosphorylation, ryanodine receptor, calsequestrin, Na+/Ca2+ exchanger, and L-type Ca2+ channel were assessed by western blot. Obese rats presented 104% increase in the adiposity index (C: 4.5±1.4 vs Ob: 9.2±1.5%) and obesity-related comorbidities compared to control rats. The left atrium diameter (C: 5.0±0.4 vs Ob: 5.5±0.5 mm) and posterior wall shortening velocity (C: 36.7±3.4 vs Ob: 41.8±3.8 mm/s) were higher in the obese group than in the control. The papillary muscle function was similar between the groups at baseline and after inotropic and lusitropic maneuvers. Obesity did not lead to changes in myocardial Ca2+ handling proteins expression. In conclusion, the hypothesis was not confirmed, since the high-saturated-fat diet-induced obese rats did not present cardiac dysfunction or impaired intracellular Ca2+ handling proteins.
DOI: 10.6084/m9.figshare.8195048.v1
title: Cardiac function and intracellular Ca2+ handling proteins are not impaired by high-saturated-fat diet-induced obesity

description: Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this study was to test the hypothesis that this dietary model of obesity leads to cardiac dysfunction resulting from alterations in the regulatory proteins of intracellular Ca2+ homeostasis. Male Wistar rats were distributed into two groups: control (C, n=18; standard diet) and obese (Ob, n=19; high-saturated-fat diet), which were fed for 33 weeks. Cardiac structure and function were evaluated using echocardiographic and isolated papillary muscle analyses. Myocardial protein expressions of sarcoplasmic reticulum Ca2+-ATPase, phospholamban (PLB), PLB serine-16 phosphorylation, PLB threonine-17 phosphorylation, ryanodine receptor, calsequestrin, Na+/Ca2+ exchanger, and L-type Ca2+ channel were assessed by western blot. Obese rats presented 104% increase in the adiposity index (C: 4.5±1.4 vs Ob: 9.2±1.5%) and obesity-related comorbidities compared to control rats. The left atrium diameter (C: 5.0±0.4 vs Ob: 5.5±0.5 mm) and posterior wall shortening velocity (C: 36.7±3.4 vs Ob: 41.8±3.8 mm/s) were higher in the obese group than in the control. The papillary muscle function was similar between the groups at baseline and after inotropic and lusitropic maneuvers. Obesity did not lead to changes in myocardial Ca2+ handling proteins expression. In conclusion, the hypothesis was not confirmed, since the high-saturated-fat diet-induced obese rats did not present cardiac dysfunction or impaired intracellular Ca2+ handling proteins.
DOI: 10.6084/m9.figshare.8195051
title: The Medical Intern after the National Curricular Guidelines of 2014: a Study in Medical School of Rio de Janeiro State

description: ABSTRACT Established in 2014, the National Curricular Guidelines (NCG) for the Undergraduate Medical Course contain several recommendations, especially for Medical Internship. Despite the recognition of the need for changes, in terms of professional training to meet the demands of the community, instituting these NCG was considered by many to be rather undemocratic. Yet, few studies have investigataed the implementation process. The objective was to analyze the Medical Internship in Medical Schools of the State of Rio de Janeiro, after institution of the NCG of 2014, according to the Course and Internship Coordinators. In 2016, the State of Rio de Janeiro had 19 medical courses, in 15 Medical Schools. Of these, nine courses participated in the research. The study population was represented by 13 participants, nine Course Coordinators and four Internship Coordinators. It is thus a sample of the universe of schools, with no intention of generalizing the results to represent the whole State. Qualitative and quantitative approaches were used. The answers of the open-ended questions were submitted to content analysis, the scale was evaluated by statistical analysis. In the view of the Coordinators participating in the research, all Medical Schools are in the process of adapting to the NGC’s determinations of 2014. The majority agrees with the mandatory inclusion of Urgency and Emergency, Primary Care and Mental Health in the Internship areas. Many difficulties were encountered in the process of implantation and/or restructuring of these activities in Internship: scarcity of practical scenarios; precariousness of existing scenarios in emergency care of the Unified Health System; lack of teachers/preceptors; and the deadline established for the implementation of the Guidelines. However, some strategies have been planned, such as the diversification of practice scenarios, the creation of elective internships, the establishment of agreements and partnerships, development of integrated activities with other Internships areas and the use of realistic simulation laboratories. The Medical Schools live a moment of curricular transformation, driven by the NCG. We must see this moment as an opportunity to revisit the Medical Internship and, possibly, to find strategies for the improvement of medical training in this privileged undergraduate space. It is believed that dissemination of the results of this research can help Medical Schools in their appropriation and implementation of the NCGs of 2014.
DOI: 10.6084/m9.figshare.8195051.v1
title: The Medical Intern after the National Curricular Guidelines of 2014: a Study in Medical School of Rio de Janeiro State

description: ABSTRACT Established in 2014, the National Curricular Guidelines (NCG) for the Undergraduate Medical Course contain several recommendations, especially for Medical Internship. Despite the recognition of the need for changes, in terms of professional training to meet the demands of the community, instituting these NCG was considered by many to be rather undemocratic. Yet, few studies have investigataed the implementation process. The objective was to analyze the Medical Internship in Medical Schools of the State of Rio de Janeiro, after institution of the NCG of 2014, according to the Course and Internship Coordinators. In 2016, the State of Rio de Janeiro had 19 medical courses, in 15 Medical Schools. Of these, nine courses participated in the research. The study population was represented by 13 participants, nine Course Coordinators and four Internship Coordinators. It is thus a sample of the universe of schools, with no intention of generalizing the results to represent the whole State. Qualitative and quantitative approaches were used. The answers of the open-ended questions were submitted to content analysis, the scale was evaluated by statistical analysis. In the view of the Coordinators participating in the research, all Medical Schools are in the process of adapting to the NGC’s determinations of 2014. The majority agrees with the mandatory inclusion of Urgency and Emergency, Primary Care and Mental Health in the Internship areas. Many difficulties were encountered in the process of implantation and/or restructuring of these activities in Internship: scarcity of practical scenarios; precariousness of existing scenarios in emergency care of the Unified Health System; lack of teachers/preceptors; and the deadline established for the implementation of the Guidelines. However, some strategies have been planned, such as the diversification of practice scenarios, the creation of elective internships, the establishment of agreements and partnerships, development of integrated activities with other Internships areas and the use of realistic simulation laboratories. The Medical Schools live a moment of curricular transformation, driven by the NCG. We must see this moment as an opportunity to revisit the Medical Internship and, possibly, to find strategies for the improvement of medical training in this privileged undergraduate space. It is believed that dissemination of the results of this research can help Medical Schools in their appropriation and implementation of the NCGs of 2014.
DOI: 10.6084/m9.figshare.8195060
title: Robot assisted radical prostatectomy in kidney transplant recipients: surgical, oncological and functional outcomes of two different robotic approaches

description: ABSTRACT  Background: To date, few series on robot-assisted radical prostatectomy (RARP) in kidney transplant recipients (KTRs) have been published.  Purpose: To report the experience of two referral centers adopting two different RARP approaches in KTRs. Surgical, oncological and functional results were primary outcomes evaluated in the study.  Material and methods: We retrospectively analyzed data from 9 KTRs who underwent transperitoneal RARP or Retzius-sparing RARP for PCa from October 2012 to April 2016. Data were reported as median and interquartile range (IQR). Pre- and postoperative outcomes were compared by non-parametric Wilcoxon signed-rank test. Significant differences were accepted when p ≤ 0.05. Overall survival was assessed using Kaplan-Meier method.  Results: Four KTRs underwent a T-RARP and 5 a RS-RARP. Patient median age was 60 (56-63) years. Charlson comorbidity index was 6 (5-6). Preoperative median PSA was 5.6 (5-15) ng / mL. Preoperative Gleason score (GS) was 6 in 5 patients, 7 (3 + 4) in 3, and 8 (4 + 4) in one. Pre- and postoperative creatinine were 1.17 (1.1; 1.4) and 1.3 (1.07; 1.57) mg / dL (p = 0.237), while eGFR was 66 (60-82) and 62 (54-81) mL / min / 1.73m2 (p = 0.553), respectively. One (11.1%) Clavien-Dindo grade II complication occurred. Two extended template lymphadenectomies were performed, both with nodal invasion. These two patients experienced a biochemical recurrence and were subjected to RT. Two patients (22.2%) had PSMs. Median follow-up was 42 months. Seven patients (77.8%) were continent, 5 (55.6%) were potent. Two (22.2%) patients died during follow-up for oncologic unrelated causes.  Conclusions: Our series suggests that both RARP approaches are safe and feasible techniques in KTRs for PCa.
DOI: 10.6084/m9.figshare.8195063
title: A quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti-double-stranded DNA IgG antibodies

description: ABSTRACT  Introduction: The detection of anti-double-stranded (ds) deoxyribonucleic acid (DNA) antibodies is one of the classification criteria for diagnosing systemic lupus erythematosus (SLE).  Objective: To describe a quantitative enzyme-linked immunosorbent assay (ELISA) for detecting anti-dsDNA immunoglobulin class G (IgG) antibodies.  Methods: The performance of ELISA was evaluated using the Crithidia luciliae indirect immunofluorescence test (CLIFT) as a reference. Anti-dsDNA IgG antibodies were screened by ELISA and CLIFT in serum samples from 127 patients with SLE, 56 patients with other diseases and 37 healthy persons. The Cochran Q test was used to compare the sensitivity and specificity of the reactions, with differences among the results being considered significant when p ≤ 0.05.  Results: ELISA had a sensitivity of 92.9% and a specificity of 94.6%, whereas the sensitivity and specificity of CLIFT were 85.8% and 100%, respectively. ELISA was significantly more sensitive than CLIFT (p = 0.0027), whereas CLIFT was significantly more specific than ELISA (p = 0.0253).  Conclusion: ELISA showed excellent results in terms of sensitivity and specificity, with a potential use in research and routine diagnostics.