cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647807
title: CIL:13456

description: Intracellular localization of GFP-GOLPH3 W81A, R90A mutant in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol (CIL# 13455) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 W81A R90A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647807.v1
title: CIL:13456

description: Intracellular localization of GFP-GOLPH3 W81A, R90A mutant in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol (CIL# 13455) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 W81A R90A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647810.v1
title: CIL:13740

description: Boi (green) no longer localizes predominantly to apical cells in Drosophila boi[e] mutant germaria (compare with CIL# 13739). Germ cells are identified using anti-Vasa (blue). Image is Fig 2C in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).
DOI: 10.6084/m9.figshare.647805
title: CIL:13454

description: Kre2-GFP is not efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing Vps74 with mutations in the sulfate-binding pocket (K178A R181A). Compare with control image CIL# 13450. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4C bottom panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4C include CIL#s 13450, 13452, 13454.
DOI: 10.6084/m9.figshare.647806
title: CIL:13455

description: Intracellular localization of GFP-GOLPH3 in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol. Control image for images showing ectopic expression of GFP-GOLPH3 with mutations in the sulfate-binding pocket (CIL#s 13456, 13457). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 wild-type/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647808.v1
title: CIL:13457

description: Intracellular localization of GFP-GOLPH3 R171A, R174A mutant in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol (CIL# 13455) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 R171A R174A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647809.v1
title: CIL:13739

description: Boi (green) localizes predominantly to apical cells in Drosophila wild-type germaria but not in boi[e] mutant germaria (CIL#13740). Germ cells are identified using anti-Vasa (blue). Image is Fig 2B in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).
DOI: 10.6084/m9.figshare.647803
title: CIL:13452

description: Kre2-GFP is not efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing Vps74 with mutations in the sulfate-binding pocket (W88A R97A). Compare with control image CIL# 13450. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4C middle panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4C include CIL#s 13450, 13452, 13454.
DOI: 10.6084/m9.figshare.647804
title: CIL:13453

description: Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol (CIL# 13449) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A K178A R181A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647806.v1
title: CIL:13455

description: Intracellular localization of GFP-GOLPH3 in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol. Control image for images showing ectopic expression of GFP-GOLPH3 with mutations in the sulfate-binding pocket (CIL#s 13456, 13457). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 wild-type/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647803.v1
title: CIL:13452

description: Kre2-GFP is not efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing Vps74 with mutations in the sulfate-binding pocket (W88A R97A). Compare with control image CIL# 13450. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4C middle panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4C include CIL#s 13450, 13452, 13454.
DOI: 10.6084/m9.figshare.647804.v1
title: CIL:13453

description: Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol (CIL# 13449) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A K178A R181A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647805.v1
title: CIL:13454

description: Kre2-GFP is not efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing Vps74 with mutations in the sulfate-binding pocket (K178A R181A). Compare with control image CIL# 13450. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4C bottom panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4C include CIL#s 13450, 13452, 13454.
DOI: 10.6084/m9.figshare.647801
title: CIL:13450

description: Kre2-GFP is efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing wild-type, untagged, Vps74. Control image for images showing that localization of Kre2-GFP is lost when mutant forms of Vps74 (mutations in the sulfate-binding pocket) are expressed (CIL#s 13452, 13454). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4C top panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4C include CIL#s 13450, 13452, 13454.
DOI: 10.6084/m9.figshare.647799
title: CIL:10495

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D stack corresponds to raw data stack with DIC: CIL# 10494 and deconvolved data stack: CIL# 10496. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647800
title: CIL:13449

description: Intracellular localization of GFP-Vps74 in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol. Control image for images showing ectopic expression of GFP-Vps74 with mutations in the sulfate-binding pocket (CIL#s 13451, 13453). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A wild-type/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647801.v1
title: CIL:13450

description: Kre2-GFP is efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing wild-type, untagged, Vps74. Control image for images showing that localization of Kre2-GFP is lost when mutant forms of Vps74 (mutations in the sulfate-binding pocket) are expressed (CIL#s 13452, 13454). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4C top panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4C include CIL#s 13450, 13452, 13454.
DOI: 10.6084/m9.figshare.647802
title: CIL:13451

description: Intracellular localization of GFP-Vps74 W88A, R97A mutant in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol (CIL# 13449) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A W88A R97A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647798
title: CIL:13889

description: Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a misaligned spindle, CIL #13873 shows an aligned spindle and CIL# 13874 shows a rebudded cell) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, middle panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.
DOI: 10.6084/m9.figshare.647800.v1
title: CIL:13449

description: Intracellular localization of GFP-Vps74 in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol. Control image for images showing ectopic expression of GFP-Vps74 with mutations in the sulfate-binding pocket (CIL#s 13451, 13453). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A wild-type/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647799.v1
title: CIL:10495

description: A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D stack corresponds to raw data stack with DIC: CIL# 10494 and deconvolved data stack: CIL# 10496. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
DOI: 10.6084/m9.figshare.647802.v1
title: CIL:13451

description: Intracellular localization of GFP-Vps74 W88A, R97A mutant in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol (CIL# 13449) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A W88A R97A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647796
title: CIL:13873

description: Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows an aligned spindle, CIL #13889 shows a misaligned spindle and CIL# 13874 shows a rebudded cell) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.
DOI: 10.6084/m9.figshare.647797
title: CIL:13874

description: Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #13873 shows an aligned spindle and CIL# 13889 shows a misaligned spindle) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.
DOI: 10.6084/m9.figshare.647797.v1
title: CIL:13874

description: Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #13873 shows an aligned spindle and CIL# 13889 shows a misaligned spindle) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.
DOI: 10.6084/m9.figshare.647795
title: CIL:13755

description: Hedgehog (Hh, red) is transcribed in apical cells of the Drosophila wild-type germarium (CIL# 13755 and this image). In the absence of boi (boi[e] mutant), Hh protein is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745), but Hh transcription is still in apical cells (CIL# 13754). Hh transcription is demonstrated by the Hh-lacZ reporter and follicle cells are highlighted using anti-Fas-3 (green). Image is Supplemental Figure S3A in J Cell Biol. 2010. 191: 943-952.