cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/zsv4j-9ds31
title: Shewanella MR1_Iron cores_Chemos

description: Raw data files of Shewanella MR1_Iron cores_Chemos
description: <strong>Tilt Series Date:</strong> 2018-08-09</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These are Shewanella oneidensis MR1 prepared by Sahand (USC) by Chemostat method grown for 2 days and chemically fixed. I (Mohammed) imaged these cells on the Polara to see if the have the iron cores in order to send them to Israel (Sharon Wolf) to perform EDX and confirm the components of the iron cores.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Chemostat 2 days growth and chemically fixed. The samples were prepared by Sahand (USC).</p>
<strong>Sample Preparation:</strong> I prepared these samples by blot force 6, blot time 3s and drain time 1. I also used the Bdellovibrio conditions (force -5, time 4 s). 10 nm Gold nanoparticles were added.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-82/rawdata/</p>SheweTilt46.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-82/rawdata/SheweTilt46.mrc"
        > <i class="download icon"></i> Download </a></p>

        SheweTilt46_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-82/3dimage_94177/SheweTilt46_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tkhbz-r5p02
title: Vibrio cholerae SAD2095 induced

description: SAD2095 (T2SS and T4 competence pili) = rpoS*, pilA S81C, lacZ::lacIq, comEA-mCherry, ΔluxO, Ptac-tfoX, Ptac- recA, ∆TCP::ZeoR, ∆MSHA::CarbR, ∆CTX::KanR, ∆pilT::TmRAnkur: "For the taxonomy ID - unfortunately the strain we sent does not have a taxonomy ID. So, you can use the generic one for V. cholerae which is "666". The strain we are working with is related to N16961, but is certainly not the same strain, so not sure if you would want to indicate that somehow? Just for reference - the strain we sent is E7946 (although it is obviously heavily modified with a number of mutations). "
description: <strong>Tilt Series Date:</strong> 2018-07-18</p>
<strong>Data Taken By:</strong> Sara Weaver</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ankur B Dalia ankdalia@indiana.edu, PhD, Assistant Professor, Indiana University. Cells grown, data collected by Sara Weaver</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streaked stock on a plate. Grew up a single colony in 3 mL LB with shaking at 37˚C. The next day, subcultured 50uL of this overnight culture into 3mL of fresh LB+100uM IPTG + 20mM MgCl2 + 10mM CaCl2 and grow at 30˚C shaking for ~4 hours.</p>
<strong>Sample Preparation:</strong> spun gold (100 µL 10 nm gold + 25 µL 5% BSA) at 15,000rpm for 20 minutes, removed supernatant.  Resuspended gold beads in 20 µL liquid culture.  Vitrobot RT and 95% humidity, 3µL of gold/sample slurry on carbon/copper Quantifoil Finder grids R2/2 (EmiTech glow discharge 50 mA 120 s)  Blot force -6, blot time 3 s, no waiting time, no drain time.  Plunged into ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2018-07-18-33/rawdata/</p>20180718_tiltseries_4.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2018-07-18-33/rawdata/20180718_tiltseries_4.mrc"
        > <i class="download icon"></i> Download </a></p>

        20180718_tiltseries_4_part100_5.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2018-07-18-33/3dimage_93526/20180718_tiltseries_4_part100_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7by5c-6z589
title: gh2-4_sq2_cell8

description: GH2-4, Sq2, IT 1.1s, phase plate
description: <strong>Tilt Series Date:</strong> 2018-05-16</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 181.0 eV/Å²,  defocus: -2.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h</p>
<strong>Sample Preparation:</strong> Backside manual blotting 5s</p>
  wnj2018-05-16-11_slicer19525.jpg: Purple: cortical sheet .   .  Green: cellulose ribbon .   .  Blue: cellulose being added to the ribbon? wnj2018-05-16-11_slicer19526.jpg: Fibrous materila underneath the cortical sheet wnj2018-05-16-11_slicer19527.jpg: Empty storage granule? wnj2018-05-16-11_slicer19528.jpg: Does the sheet closely follow where synthesis is occuring? wnj2018-05-16-11_slicer19529.jpg: Does the sheet closely follow where synthesis is occuring? wnj2018-05-16-11_slicer19530.jpg: Does the sheet closely follow where synthesis is occuring?
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-11/rawdata/</p>gh2-4_sq2_cell8.mrc,  Tilt Series (Pixel Size 0.43 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-11/rawdata/gh2-4_sq2_cell8.mrc"
        > <i class="download icon"></i> Download </a></p>

        gh2-4_sq2_cell8_part61_5.rec,  Reconstruction (Pixel Size 0.86 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-11/3dimage_92598/gh2-4_sq2_cell8_part61_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q2hx6-6xt27
title: BacillusSubtilis_293

description: Bacterial nanotubes (like what is described in Sigal ben Yehuda and Ilan Rosenshine work).
description: <strong>Tilt Series Date:</strong> 2020-02-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> The sample was manually blotted to increase the chance of finding an intercellular nanotube.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-133/rawdata/</p>Bsubtilis_293_134_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-133/rawdata/Bsubtilis_293_134_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_293_134_af_part41_4.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-16-133/3dimage_111603/Bsubtilis_293_134_af_part41_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mtnh3-bxq30
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-95/rawdata/</p>Hpylori_DFliF_FISE_095.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-95/rawdata/Hpylori_DFliF_FISE_095.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_095_part41_1.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-95/3dimage_113673/Hpylori_DFliF_FISE_095_part41_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nwqg9-mjz84
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-143/rawdata/</p>Hpylori_DFliF_FISE_146.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-143/rawdata/Hpylori_DFliF_FISE_146.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_146_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-143/3dimage_113767/Hpylori_DFliF_FISE_146_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q262r-fze96
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-04-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin was spun down and resuspended in fresh LB with IPTG/ampicilin and grown for 3 hours. The cells was idle for 30 min at RT during grid preperations prior to freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-08-20/rawdata/</p>20150408_AK004_10019.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-08-20/rawdata/20150408_AK004_10019.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150408_AK004_10019_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-08-20/3dimage_53711/20150408_AK004_10019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/4nh-qtd-k4g
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qqn-82n-0t6
title: Cnidaria

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/scv-cc6-vfb
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/t6k-17a-2bc
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4jy-g3r-d16
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4z0-219-9d0
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/khj-rc9-kz0
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/3pd-nxd-nyk
title: Cnidaria

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vq2-1ql-nmx
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/tqnfb-w1a93
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-08-15</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2017-08-15-44_slicer18767.jpg: Top view of T4SS?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-44/rawdata/</p>Cb_20170816_Cb1pH4d50043.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-44/rawdata/Cb_20170816_Cb1pH4d50043.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_20170816_Cb1pH4d50043_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-44/3dimage_88030/Cb_20170816_Cb1pH4d50043_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/lwq-6e6-c1p
title: Polychelidae Wood-Mason, 1875

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/xyx-ath-dc1
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/4j5x0-jd776
title: Agro_AD1231

description: AD1231: pAD1361/A136; Carbenicillin 50;  virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.
description: <strong>Tilt Series Date:</strong> 2015-08-03</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 155.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow culture in AB medium (pH 7) at 30C. For induction, use 375 µl of a decent ON culture to inoculate 5 ml AB mes pH 5.8 medium containing 100 µM Acetosyringone and antibiotic (Cb 50). Grow at 20C for 18 hr. Concentrate cells 5 times by centrifuging. Freeze cells.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK III, MK IV was not working) settings: 100% humidity, 4µl sample, 5% BSA treated gold. Blot offset -5, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  dga2015-08-03-63_slicer19990.jpg: Rod
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-63/3dimage_60099/</p>20150803_1231_20022_part121_15.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-63/3dimage_60099/20150803_1231_20022_part121_15.rec"
        > <i class="download icon"></i> Download </a></p>

        20150803_1231_20022.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-63/rawdata/20150803_1231_20022.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/2w7-56e-3kq
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4gf-558-ls9
title: Coleoidea Bather, 1888

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/g87-rq5-wad
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/bwa-4sv-n43
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/pm0-s5d-pef
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/sh1-91m-y63
title: Coleoidea Bather, 1888

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.