cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0q23z5m
title: CIL:36777, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Detail of the junction between the ribbed wall and the right side of the cytopharynx area. The ribbed wall is covered by alveoli and the ribs are produced by small narrow ridges similar to the larger ridges covering the whole cell body. Two arrays of microtubules are present in this zone, clumps of hexagonally packed bundles lying under the ridges and rows of microtubules that lie under the single cytopharyngeal membrane. There is some suggestion that these rows of microtubules may associate together to form the bundles. Tips of cilia in cross section give 4 views of how the tips of cilia end, the 9-doublet first become 9 singlets and then these are lost one by one leaving only the two central singlets at the tip of a cilium. Sections of the cytostomal cord are also present under the rib wall. This is the cord that wraps around the cytopharynx. TEM taken on 7/27/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 23,000X. Bar = 0.2µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39157) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j06m35tg
title: CIL:36750, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Quick-freeze deep-etch image of a DV-III containing paracrystalline sheets in its lumen. The sheets have parallel rows of subunits spaced at 20nm center-to-center but the chemical nature of the sheets is unknown; they do not appear to be acid phosphatase positive. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0js9pdd
title: CIL:36748, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: Detail of fractured early Digestion Vacuole-III membrane showing the many IMPs on the P-fracture faces of the lysosomal membranes that had just fused with the DV. The membrane will smooth out as the phagolysosome expands osmotically. The membrane in the upper right corner has fewer IMPs and may be a remnant of the DV-II membrane. (For relative numbers of IMPs in the different DV stages see Allen and Staehelin, J. Cell Biol. 89:9-20, 1981). TEM taken on 9/27/76 by R. Allen with JEM-100B operating at 80kV. Neg. 31,193X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j05t3j58
title: CIL:36760, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38896) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Septa of bordering alveoli when lying in the plane of section exhibit pores at their outer margins closest to the plasma membrane. This shows that there is physical continuity between the lumens of alveoli. Basal bodies and fibrous systems are found in the cellular cortex beneath the ridges. Cacodylate buffered fixative. TEM taken on 11/2/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.5µm. Published in J. Cell Biol. 49:1-20, 1971. Adapted with permission.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0hd7tmw
title: CIL:36759, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A cell with a flat alveolar sac system. It is not clear why some cells have expanded alveoli and others have flattened alveoli. Cacodylate buffered fixative. TEM taken on 2/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38895) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j03t9g53
title: CIL:36757, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38893) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: A higher magnification view that shows the bilayer nature of the ciliary membrane, plasma membrane and the outer and inner (alveolar sac membranes of the alveoli. The 9-doublet + 2-singlet microtubular axonemal system of the cilium is seen in cross section. Two dynein arms extend from each doublet. Cacodylate buffered fixative. TEM taken on 9/27/68 by R. Allen with Philips 300 operating at 60kV. Neg. 60,400X. Bar = 0.1µm.
DOI: 10.6075/j0p849td
title: CIL:36755, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Paramecium caudatum is a peniculine oligohymenophoran ciliate that has numerous rows of cilia called kineties that run along the posterior to the anterior axis on the cell‚Äôs dorsal side but curve around the oral region on the cell‚Äôs ventral side and end to form the anterior and posterior suture lines that extend from the oral region to the tips of the cell. This is a cross-section of P. caudatum near its anterior end showing a layer of trichocysts that lie under the cell surface. The surface of the cell is sculpted into ridges and the cilia arise from the bases of the concave invaginations. Each concavity represents a section through one kinety so that in this micrograph approximately 58 kineties can be seen to surround the cell near its anterior end. At this low magnification one can also see large numbers of medium-opaque mitochondria near the cell surface and interspersed between the trichocysts. Collidine-buffered fixation. TEM taken on 1/18/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,750X. Bar = 1µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39112) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0251gtq
title: CIL:36734, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Quick-freeze deep-etch of acidosomes show the results of carrier vesicle fusion with the acidosomes. Both the E-face and P-face of the acidosomes appear to have two areas, the larger area on the E-face has few IMPs but has pits left by the removal of IMPs during fracturing and another area that is smooth with fewer pits that resembles a fused carrier vesicle membrane. A line marking the fusion site between these two vesicles is evident. The P-face of the acidosome has many IMPs while the fused carrier vesicle membrane is smooth. Presumably these two membrane areas will mix with time. Thus acidosomes, which are somewhat similar in appearance to multivesicular bodies in higher organisms, are late endosomes and deliver their membrane, their contents and their V-ATPase complexes to the phagosome to transform the phagosome to the phagoacidosome. TEM taken on 7/29/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40563) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0222sfv
title: CIL:36733, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are first exposed to an acid environment in the acidosomes and phagoacidosomes and then to digestion in the phagolysosomes. (For a discussion of acidosome development see Allen, Ma and Fok, J. Cell Sci. 106:411-422, 1993). TEM taken on 7/19/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.
description: A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40562) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0x34w3g
title: CIL:36741, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Immunogold label specific for L1 antigen on DV-II. Monoclonal antibody to antigen L1 specifically labels DV-II and acidosomes but not lysosomes. Lysosomes are osmotically active so they tend to shrink when the cell is exposed to hyperosmotic fixative as occurred here. This vacuole was 5-8 min. old and contained 0.8µm latex beads. TEM taken on 3/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.
description: Glutaraldehyde fixation followed dehydration in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40564) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0gt5ks9
title: CIL:36732, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Acidosomes originate from the biosynthetic pathway that includes the ER, the Golgi stack and the trans-Golgi network (TGN). Flattened vesicles from the TGN round up into spherical vesicles and bind to microtubules (not shown here) that come from the cytopharynx. While moving along these microtubules they come into contact with the carrier vesicles that also bind to the same microtubules. TEM taken on 7/19/88 by C. Schroeder with Zeiss 10A TEM. Neg. 24,800X. Bar = 0.2µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.
description: A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40561) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0fx78d4
title: CIL:36704, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: A view of cross sections of the finger-like extensions of the alveoli that bind to the cytostomal cord. Alveoli store calcium (see Stelly et al., J. Cell Biol. 113:103-112, 1991) and calcium release causes the infraciliary lattice to contract. Presumably a controlled release of calcium from these fingers will result in the contraction of the cytostomal cord which is an extension of the infraciliary lattice. Such a contraction could either promote discoidal vesicle fusion with the cytopharyngeal membrane or initiate release of the nascent vacuole from the cytopharynx. TEM taken on 4/4/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0833qmv
title: CIL:36709, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Quick-freeze deep-etch image of rows of carrier vesicles lined up along the cytopharyngeal microtubular ribbons. These vesicles arise from early endosomes as clathrin coated vesicles and are about 100nm in diameter. Vesicles lose their coats before binding with the dynein motors that link them to the microtubules. They tend to lie on the anterior side of the ribbons along with the discoidal vesicles. Carrier vesicles eventually dock on acidosomes and fuse with these. Acidosomes are late endosomes. TEM taken on 5/24/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40506) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).Small part published in J. Cell Biol. 111:2553-2562, 1990. Adapted with permission.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed