cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647896.v1
title: CIL:13374

description: Z-series of fluorescence images of a Hela cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). This image is the control for the Bod-1 depletion experiment illustrated in CIL: 13372. Bod-1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.
DOI: 10.6084/m9.figshare.647892
title: CIL:12580

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the anterior and posterior midgut. Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:19118, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647893
title: CIL:12581

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the midgut and associated trachea. Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:19119, from the same image group, is a labeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820-826.
DOI: 10.6084/m9.figshare.647894.v1
title: CIL:12591

description: Micrograph of a semi-thick (1-2 µm) section of an infected maize leaf, with silver-enhanced immunogold labeling, that shows dark staining of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates in phloem tissue. The section was counter stained with toluidine blue. CIL:19129, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. dditional related images from this contributor are available in the Library.
DOI: 10.6084/m9.figshare.647890
title: CIL:12416

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16309. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647891
title: CIL:12417

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16311. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647892.v1
title: CIL:12580

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the anterior and posterior midgut. Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:19118, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.
DOI: 10.6084/m9.figshare.647893.v1
title: CIL:12581

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the midgut and associated trachea. Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:19119, from the same image group, is a labeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820-826.
DOI: 10.6084/m9.figshare.647889
title: CIL:12415

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16310. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647890.v1
title: CIL:12416

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16309. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647891.v1
title: CIL:12417

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16311. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647887
title: CIL:12311

description: Detail of cytopharyngeal vesicles. TEM taken on 3/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7665) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647888
title: CIL:12337

description: The transition from 3 to 2 rows of cilia can be seen in this view of the AZM. TEM taken on 7/24/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,370X. Bar = 5µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image is available in the library at (CIL:38890) for qualitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647888.v1
title: CIL:12337

description: The transition from 3 to 2 rows of cilia can be seen in this view of the AZM. TEM taken on 7/24/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,370X. Bar = 5µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image is available in the library at (CIL:38890) for qualitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647885
title: CIL:11959

description: This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. The spindle and its orientation are made visible by the use of polarizing optics, and in this sequence, rotation of the spindle causes a temporary reversal of its contrast from bright to dark. When the needle is withdrawn, the spindle returns to its original position, but the opposite pole is now attached to the cortex.
DOI: 10.6084/m9.figshare.647886
title: CIL:12308

description: The top of the cytopharyngeal basket showing a closed cytostome where food will be engulfed. In the lab Nassula was fed Phormidium inundatum, a cyanobacterium. Ingested food is seen in food vacuoles as intensely stained blobs which are in various stages of digestion. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X. Bar = 1µm.
DOI: 10.6084/m9.figshare.647886.v1
title: CIL:12308

description: The top of the cytopharyngeal basket showing a closed cytostome where food will be engulfed. In the lab Nassula was fed Phormidium inundatum, a cyanobacterium. Ingested food is seen in food vacuoles as intensely stained blobs which are in various stages of digestion. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X. Bar = 1µm.
DOI: 10.6084/m9.figshare.647889.v1
title: CIL:12415

description: The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16310. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.
DOI: 10.6084/m9.figshare.647884
title: CIL:11820

description: Dorsal cell movements during zebrafish gastrulation includes a combination of epiboly, involution, and convergence and extension along the embryonic axis. Note that videos CIL 11818, 11819, and 11820 are all of the same sequence. 11819 is the raw data, 11818 can be viewed in 3D when wearing red/blue glasses and 11820 has some cells marked with colored dots for tracking the movement and timing of cell involution and convergence.. Cells closer to the margin involute first and end up in more anterior locations in the hypoblast, while cell involuting later move to more posterior locations along the anterior-posterior axis. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.647885.v1
title: CIL:11959

description: This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. The spindle and its orientation are made visible by the use of polarizing optics, and in this sequence, rotation of the spindle causes a temporary reversal of its contrast from bright to dark. When the needle is withdrawn, the spindle returns to its original position, but the opposite pole is now attached to the cortex.
DOI: 10.6084/m9.figshare.647887.v1
title: CIL:12311

description: Detail of cytopharyngeal vesicles. TEM taken on 3/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7665) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6084/m9.figshare.647881
title: CIL:10441

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation. The coils appear to stack in loose hexagonal arrays (CIL:9301). The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. In this micrograph, large numbers of intermediate filaments are seen; they are evidently very close to the inner surface of the plasma membrane, where they insert into electron-dense desmosomal plagues. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).
DOI: 10.6084/m9.figshare.647883
title: CIL:11819

description: Dorsal cell movements during zebrafish gastrulation includes a combination of epiboly, involution, and convergence and extension along the embryonic axis. Note that videos CIL 11818, 11819, and 11820 are all of the same sequence. 11819 is the raw data, 11818 can be viewed in 3D when wearing red/blue glasses and 11820 has some cells marked with colored dots for tracking the movement and timing of cell involution and convergence.. Cells closer to the margin involute first and end up in more anterior locations in the hypoblast, while cell involuting later move to more posterior locations along the anterior-posterior axis. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.647882
title: CIL:11818

description: Dorsal cell movements during zebrafish gastrulation includes a combination of epiboly, involution, and convergence and extension along the embryonic axis. Note that videos CIL 11818, 11819, and 11820 are all of the same sequence. 11819 is the raw data, 11818 can be viewed in 3D when wearing red/blue glasses and 11820 has some cells marked with colored dots for tracking the movement and timing of cell involution and convergence.. Cells closer to the margin involute first and end up in more anterior locations in the hypoblast, while cell involuting later move to more posterior locations along the anterior-posterior axis. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.647882.v1
title: CIL:11818

description: Dorsal cell movements during zebrafish gastrulation includes a combination of epiboly, involution, and convergence and extension along the embryonic axis. Note that videos CIL 11818, 11819, and 11820 are all of the same sequence. 11819 is the raw data, 11818 can be viewed in 3D when wearing red/blue glasses and 11820 has some cells marked with colored dots for tracking the movement and timing of cell involution and convergence.. Cells closer to the margin involute first and end up in more anterior locations in the hypoblast, while cell involuting later move to more posterior locations along the anterior-posterior axis. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.647883.v1
title: CIL:11819

description: Dorsal cell movements during zebrafish gastrulation includes a combination of epiboly, involution, and convergence and extension along the embryonic axis. Note that videos CIL 11818, 11819, and 11820 are all of the same sequence. 11819 is the raw data, 11818 can be viewed in 3D when wearing red/blue glasses and 11820 has some cells marked with colored dots for tracking the movement and timing of cell involution and convergence.. Cells closer to the margin involute first and end up in more anterior locations in the hypoblast, while cell involuting later move to more posterior locations along the anterior-posterior axis. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.647884.v1
title: CIL:11820

description: Dorsal cell movements during zebrafish gastrulation includes a combination of epiboly, involution, and convergence and extension along the embryonic axis. Note that videos CIL 11818, 11819, and 11820 are all of the same sequence. 11819 is the raw data, 11818 can be viewed in 3D when wearing red/blue glasses and 11820 has some cells marked with colored dots for tracking the movement and timing of cell involution and convergence.. Cells closer to the margin involute first and end up in more anterior locations in the hypoblast, while cell involuting later move to more posterior locations along the anterior-posterior axis. Video made available by Mark Cooper through Zebrafish - The Living Laboratory.
DOI: 10.6084/m9.figshare.647879
title: CIL:10434

description: This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation. The coils appear to stack in loose hexagonal arrays (CIL:9301). The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. In this micrograph, large numbers of intermediate filaments are seen; they are evidently very close to the inner surface of the plasma membrane, where they insert into electron-dense desmosomal plagues. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).