DOI: 10.5061/dryad.pf012v0
title: Data from: Cardiomyocyte mechanodynamics under conditions of actin remodelling

description: The mechanical performance of cardiomyocytes is an important indicator of
 their maturation state and of primary importance for the development of
 therapies based on cardiac stem-cells. As the mechanical analysis of
 adherent cells at high-throughput remains challenging, we explore the
 applicability of real-time deformability cytometry (RT-DC) to probe
 cardiomyocytes in suspension. RT-DC is a microfluidic technology allowing
 for real-time mechanical analysis of thousands of cells with a throughput
 exceeding 1,000 cells per second. For cardiomyocytes derived from human
 induced pluripotent stem cells, we determined a Young’s modulus of
 1.250.08 kPa which is in close range to previous reports. Upon
 challenging the cytoskeleton with cytochalasin D (CytoD) to induce
 filamentous actin depolymerization, we distinguish three different regimes
 in cellular elasticity. Transitions are observed below 10 nM and above 103
 nM and are characterized by a decrease in the Young’s modulus. These
 regimes can be linked to cytoskeletal and sarcomeric actin contributions
 by cardiomyocyte contractility measurements at varying CytoD
 concentrations, where we observe a significant reduction in pulse duration
 only above 103 nM while no change is found for compound exposure at lower
 concentrations. Comparing our results to mechanical cell measurements
 using atomic force microscopy we demonstrate for the first time the
 feasibility of using a microfluidic technique to measure mechanical
 properties of large samples of adherent cells while linking our results to
 the composition of the cytoskeletal network.
description: Figure1
 RT-DCThis file contains the
 data of mechanical phenotype for cardiomyocytes measured by
 RT_DCFigure1_RT_DC_M1_data.tdmsFigure 1 Fluorescence controlThis file contains the unlabelled control.control 3 - M1.fcsFigure 1 Fluorescence DNAThis file contains the DNA stainsample 1uL - M1.fcsFigure 2 RT-DC DMSO 1 controlThis file contains the DMSO 1 control of the RT-DC measurementFigure2_RT_DC_DMSO1_M1_data.tdmsFigure 2 RT-DC DMSO 2 controlDMSO control 2 for RT-DC measurementsFigure2_RT_DC_DMSO2_M1_data.tdmsFigure 2 RT-DC CytoD 1 0.01uMRT-DC CytoD 1 0.01uM dataFigure2_RT_DC_CytoD1_0_01_M1_data.tdmsFigure 2 RT-DC CytoD 2 0.01 uMRT-DC data for CytoD repeat 2 0.01 uM measurementFigure2_RT_DC_CytoD2_0_01_M1_data.tdmsFigure 2 RT-DC CytoD 1 0.1uMRT-DC data from CytoD 1 0.1uM measurementFigure2_RT_DC_CytoD1_0_1_M1_data.tdmsFigure 2 RT-DC CytoD 2 0.1uMRT-DC data from CytoD 2 0.1uM measurementFigure2_RT_DC_CytoD2_0_1_M1_data.tdmsFigure 2 RT-DC CytoD 1 1uMRT-DC data from CytoD 1 1uM measurementFigure2_RT_DC_CytoD1_1_M1_data.tdmsFigure 2 RT-DC CytoD 2 1uMRT-DC data from CytoD 2 1uM measurementFigure2_RT_DC_CytoD2_1_M1_data.tdmsFigure 2 RT-DC CytoD 1 10uMRT-DC data from CytoD 1 10uM measurementFigure2_RT_DC_CytoD1_10_M1_data.tdmsFigure 2 RT-DC CytoD 2 10uMRT-DC data from CytoD 2 10uM measurementFigure2_RT_DC_CytoD2_10_M1_data.tdmsFigure 2 AFM HistogramFigure 2 AFM data histogramFigure2_AFM_Histogram.datFigure 2 AFM comparisonFigure 2 data AFM comparisonFigure2_AFM_Comparison.csvFigure 2 contractilityFigure 2 data cardiomyocyte contractilityFigure2_Contractility_Data.txt