cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0pc31cx
title: CIL:34745, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: A high resolution view of the left side of the buccal cavity. It is covered by the plasma membrane, alveoli and a single partial layer of microtubules. Specialized cytoplasm lines the posterior end of this border. Oral ribs contain microtubular ribbons and interdigitate with alveolar tubes. TEM taken on 8/21/67 by R. Allen with Philips 200 operating at 60kV. Neg. 12,400X.The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0st7nt4
title: CIL:34744, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: A high resolution view of a longitudinal section through the buccal cavity of the oral region showing portions of 3 membranelles (1, 2 and 3): each membranelle consists of 3 rows of cilia. The oral ribs are seen on the right side of the buccal cavity and are underlain by a filamentous reticulum and by the endoplasmic reticulum. A specialized cytoplasm covers the posterior left side of the buccal cavity and a single layer of microtubules covers the left side. TEM taken on 2/9/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0t152kk
title: CIL:34743, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: High resolution view of early endosomes with coated evaginations at their margins.TEM taken on 8/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
DOI: 10.6075/j0j965cs
title: CIL:34613, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: An image of the nucleoli of the macronucleus. Nucleoli have a core of DNA that contains the genes that are transcribed into ribosomal RNA. Assembly of the ribosomal subunits from this processed RNA and from proteins transported into the nucleus through the pores occurs in the nucleoli. A concentric stack of rough ER encircles a mitochondrion. TEM taken on 8/16/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Bar = 1µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34537) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j09p30mb
title: CIL:34611, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Cross section the 9 + 2 axoneme of the cilium of Tetrahymena. The plasma membrane covers the axoneme and is continuous with the plasma membrane covering the cell body. Each doublet tilts inward to the tangent of the axonemal cylinder. Two arms, an inner arm and outer arm, extend from the complete microtubule of each doublet. These arms have ATPase activity and are called dyneins. They cause one doublet to slide against the adjacent doublet. Other protein links in the axoneme are elastic and induce the characteristic bending of the cilium and prevent excess sliding as the doublets slide past each other. TEM taken on 7/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34601) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0wd3z7h
title: CIL:34604, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: This cross section is near the proximal end of the cilium. Triangular densities link the doublets to the membrane. TEM taken 7/23/65 by R. Allen with RCA EMU3F operating at 50kV. Adapted with permission. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
DOI: 10.6075/j06m35rk
title: CIL:34736, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: An image of the cortex of Tetrahymena showing a longitudinal microtubular ribbon sandwiched between the alveoli and the epiplasm in the crest of the pellicular ridge. A parasomal sac, the distal end of basal body, the proximal end of cilium, a mucocyst, and mitochondrion are all visible in this micrograph. TEM taken on 1/12/71 by M. Aihara with Hitachi HU11A operating at 75kV. Neg. 17,000X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34727) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j05m64nn
title: CIL:34735, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: A micrograph of Tetrahymena showing a transverse ribbon starting from the anterior right side of the proximal end of a basal body (out of section plane) and crossing under the pellicle to end under the longitudinal ribbon in the adjacent ridge crest. The epiplasm separates the two ribbons. Mitochondria often lie in ridges and the crescent end of kinetodesmal fiber, a dictyosome, and the endosomal membrane is seen. TEM taken on 8/25/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34728) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0np23bq
title: CIL:34731, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: High resolution image of mucocysts which have a herringbone pattern of subunits and are surrounded by a membrane that fuses with the plasma membrane. When the mucocyst content is extruded the content expands (see Hausmann, Protistologica 8:401-412, 1972 for details. TEM taken on 8/12/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j04j0d22
title: CIL:34727, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A high resolution image of a longitudinal microtubular ribbon sandwiched between the alveoli and the epiplasm in the crest of the pellicular ridge. A parasomal sac, the distal end of basal body, the proximal end of cilium, a mucocyst, and mitochondrion are all visible in this micrograph. TEM taken on 1/12/71 by M. Aihara with Hitachi HU11A operating at 75kV. Neg. 17,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0086478
title: CIL:34742, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: High resolution micrograph of the cortex of Tetrahymena. One parasomal sac lies anterior to each basal body of the somatic ciliature. A parasomal sac is an indentation of the plasma membrane that penetrates into the cytosol past the alveolus. Most sacs end as a coated pit. However, as seen in this micrograph, they can also give rise to more extensive tubules and vesicles. These vesicles pinch off into the cytosol to become early endosomes in a process called endocytosis where they lose their coat and fuse with a flattened smooth-membraned cisterna that is the early endosome. lip, lipid droplet. TEM taken on 7/17/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j04f1pnb
title: CIL:34741, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: High resolution image showing that one parasomal sac lies anterior to each basal body of the somatic ciliature. A parasomal sac is an indentation of the plasma membrane that penetrates into the cytosol past the alveolus. Most sacs end as a coated pit. However, as seen in this micrograph, they can also give rise to more extensive tubules and vesicles. These vesicles pinch off into the cytosol to become early endosomes in a process called endocytosis where they lose their coat and fuse with a flattened smooth-membraned cisterna that is the early endosome. TEM taken on 7/17/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0gf0tkt
title: CIL:34740, Rattus, multipolar neuron, glial cell. In Cell Image Library

description: Z-stack through the CA region of the hippocampus of adult rat, immunostained to reveal dendrites of neurons (red), astroglia (green) and all nuclei (blue). This kind of approach can reveal the spatial relationships between the nonneuronal glial cells and neurons.
description: Tissue was perfused with 4% paraformaldehyde in PBS, postfixed overnight, sectioned at a thickness of 25 µm,incubated in blocking solution (0/25% lambda carageenen, 1%bovine serum albumin, and 0.5% Triton X-100 in 0.01M PBS) and immunostained using antibodies to the dendritically-localized microtubule protein MAP2 (polyclonal Ab266 from S. Halpain), and the astroglial cytoskeletal protein GFAP (glial fibrillary acidic protein, monoclonal clone G-A-5, Sigma) followed by fluorescently-conjugated secondary antibodies (anti-mouse Alexa 488, 1:400; anti-rabbit Alexa 546, both from Molecular Probes), and incubated in 1:1000 Hoescht33258 to stain nuclei. A multiframe data set of 5x3x20(X,Y,Z) was acquired using the Leica SP5 confocal microscope with line-by-line sequential scanning, a 63X HCX PL APOCHROMAT, 1.32NA, with 1.7 zoom at z-intervals of 0.5 µm, and saved as a tif image stack.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: Hoechst 33258; Alexa Fluor 488; Alexa Fluor 546
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0bp02vg
title: CIL:34739, Rattus, multipolar neuron, glial cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization methods: Hoechst 33258; Alexa Fluor 488; Alexa Fluor 546
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Tissue was perfused with 4% paraformaldehyde in PBS, postfixed overnight, sectioned at a thickness of 25 µm,incubated in blocking solution (0/25% lambda carageenen, 1%bovine serum albumin, and 0.5% Triton X-100 in 0.01M PBS) and immunostained using antibodies to the dendritically-localized microtubule protein MAP2 (polyclonal Ab266 from S. Halpain), and the astroglial cytoskeletal protein GFAP (glial fibrillary acidic protein, monoclonal clone G-A-5, Sigma) followed by fluorescently-conjugated secondary antibodies (anti-mouse Alexa 488, 1:400; anti-rabbit Alexa 546, both from Molecular Probes), and incubated in 1:1000 Hoescht33258 to stain nuclei. A multiframe data set of 5x3x20(X,Y,Z) was acquired using the Leica SP5 confocal microscope with line-by-line sequential scanning, a 63X HCX PL APOCHROMAT, 1.32NA with 1.7 zoom, at z-intervals of 0.5 µm, and saved as a tif image.
description: Maximum projection of a multipanel, tiled image through the CA region of the hippocampus of adult rat, immunostained to reveal dendrites of neurons (red), astroglia (green) and all nuclei (blue). This kind of approach can reveal the spatial relationships between the nonneuronal glial cells and neurons.
DOI: 10.6075/j070802j
title: CIL:35275, Danio rerio, embryonic cell. In Cell Image Library

description: Zebrafish development from extension of head and tail rudiment during axiation, somitogenesis. Video made avaialable through Mark Cooper and Zebrafish - The Living Laboratory.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data