DOI: 10.20350/digitalcsic/17484
title: Dataset - Cr3+-doped gamma-and beta-Gallium Oxide Nanoprobes for Bioimaging: Synthesis, Persistent luminescence and Biocompatibility

description: File List: Fig 1b: Size histogram of undoped gamma-Ga2O3 particles Fig 1c: DLS curve in aqueous suspension of undoped gamma-Ga2O3 particles Fig 1d : XRD pattern of undoped gamma-Ga2O3 particles Fig 1f: TG curve of undoped gamma-Ga2O3 particles Fig 2e: Particle size distributions and normal fitted curves of gamma-Ga2O3 particles doped with 0.1 %, 0.5 %, and 2.0 % Cr3+. Fig 2f: DLS curves of particles shown in (a) to (d) suspended in distilled water. Fig 3a: PLE spectrum of gamma-Ga2O3: 0.5 % Cr3+. Fig 3b: PL spectra of gamma-Ga2O3: x % Cr3+. Fig 3c: PersL decay curves of gamma-Ga2O3: x% Cr3+ after being illuminated with UV light (260 nm) for 5 min Fig 3d: PersL decay curves of gamma-Ga2O3: 2.0 % Cr3+ after being illuminated with UV light (260 nm) for 5 min recorded at two different emission wavelengths Fig 4b: FTIR spectra of the NPs@SiO2 and final NPs Fig 4c: Particle size distributions of the NPs@SiO2 and final NPs Fig 4d: DLS curves in aqueous suspension (pH 6) (d) of the NPs@SiO2 and final NPs Fig 4f: XRD pattern of the final NPs Fig 5a, 5b and 5c:Comparison of the photoluminescence properties of -Ga2O3 and gamma-Ga2O3 NPs. a) Absolute PL spectra, b) Normalized PL spectra, and c) PersL decay curves Fig 6a: PLE and PersL excitation spectra of beta-Ga2O3 NPs Fig 6b: PL and PersL emission spectra of beta-Ga2O3 NPs Fig 8a: DLS curves of beta-Ga2O3:2 % Cr3+ NPs dispersed in MES. Fig 8b: Zavg and PdI values as a function of time, obtained from DLS measurements on beta-Ga2O3:2 % Cr3+ NPs dispersed in MES. Fig 10: Cell viability assay of MIA PaCa-2 cell line exposed to different concentrations of beta-Ga2O3:2 % Cr3+ NPs for 24 h. Fig 11d: EDX spectrum of the region marked with a quadrangle in Fig 11b. Fig 12: Viability (a) and brood size (b) of N2 worms incubated at 20°C with different concentrations (mg·mL-1) of beta-Ga2O3:2 % Cr3+ NPs for 24h.
description: Persistent luminescent nanophosphors that emit in the near infrared region are promising nanoprobes for in-vivo bioimaging. Although Cr3+-doped zinc gallate nanoparticles have been widely studied as in-vivo bioimaging nanoprobes due to their NIR persistent luminescence emission at 695 nm, the simpler Cr3+-doped gallium oxide system has been less explored despite its deeper NIR emission (760 nm), which favours tissue penetration. This is likely due the lack of synthesis methods that render Ga2O3-based nanoparticles suitable for in-vivo applications. In this paper, we report a novel method for the synthesis of uniform and hydrophilic -Ga2O3:Cr3+ nanoparticles, whose photoluminescence and persistent luminescence were optimized by adjusting their Cr3+ content. Such properties were further greatly improved through an annealing process at high temperature, which resulted in the transformation of its crystal structure into the -phase. The obtained -Ga2O3:Cr3+ nanoparticles were colloidally stable in a physiological pH simulator medium and were nontoxic for cells. Finally, we also studied, for the first time in literature, the in-vivo biocompatibility of such nanoparticles using a C. elegans animal model, finding that their morbidity and reproductive toxicity were negligible. In summary, the reported nanoparticles are excellent candidates for their use as a NIR persistent luminescence probes for in-vivo bioimaging.
description: File List: Fig 1b: Size histogram of undoped gamma-Ga2O3 particles Fig 1c: DLS curve in aqueous suspension of undoped gamma-Ga2O3 particles Fig 1d : XRD pattern of undoped gamma-Ga2O3 particles Fig 1f: TG curve of undoped gamma-Ga2O3 particles Fig 2e: Particle size distributions and normal fitted curves of gamma-Ga2O3 particles doped with 0.1 %, 0.5 %, and 2.0 % Cr3+. Fig 2f: DLS curves of particles shown in (a) to (d) suspended in distilled water. Fig 3a: PLE spectrum of gamma-Ga2O3: 0.5 % Cr3+. Fig 3b: PL spectra of gamma-Ga2O3: x % Cr3+. Fig 3c: PersL decay curves of gamma-Ga2O3: x% Cr3+ after being illuminated with UV light (260 nm) for 5 min Fig 3d: PersL decay curves of gamma-Ga2O3: 2.0 % Cr3+ after being illuminated with UV light (260 nm) for 5 min recorded at two different emission wavelengths Fig 4b: FTIR spectra of the NPs@SiO2 and final NPs Fig 4c: Particle size distributions of the NPs@SiO2 and final NPs Fig 4d: DLS curves in aqueous suspension (pH 6) (d) of the NPs@SiO2 and final NPs Fig 4f: XRD pattern of the final NPs Fig 5a, 5b and 5c:Comparison of the photoluminescence properties of -Ga2O3 and gamma-Ga2O3 NPs. a) Absolute PL spectra, b) Normalized PL spectra, and c) PersL decay curves Fig 6a: PLE and PersL excitation spectra of beta-Ga2O3 NPs Fig 6b: PL and PersL emission spectra of beta-Ga2O3 NPs Fig 8a: DLS curves of beta-Ga2O3:2 % Cr3+ NPs dispersed in MES. Fig 8b: Zavg and PdI values as a function of time, obtained from DLS measurements on beta-Ga2O3:2 % Cr3+ NPs dispersed in MES. Fig 10: Cell viability assay of MIA PaCa-2 cell line exposed to different concentrations of beta-Ga2O3:2 % Cr3+ NPs for 24 h. Fig 11d: EDX spectrum of the region marked with a quadrangle in Fig 11b. Fig 12: Viability (a) and brood size (b) of N2 worms incubated at 20°C with different concentrations (mg·mL-1) of beta-Ga2O3:2 % Cr3+ NPs for 24h.