cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1568657
title: Low expression of ITIH5 in adenocarcinoma of the lung is associated with unfavorable patients' outcome

description: Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is supposed to be involved in extracellular matrix stability and thus may play a key role in the inhibition of tumor progression. The current study is the first to analyze in depth ITIH5 expression and DNA methylation, as well as its potential clinical impact in non-small-cell lung carcinoma (NSCLC). We examined <i>ITIH5</i> mRNA expression in tumor and adjacent normal lung tissue specimens of NSCLC patients. In addition, methylation frequency of the <i>ITIH5</i> promoter was investigated using methylation-specific PCR and pyrosequencing. Significance of our data was validated by independent data sets from The Cancer Genome Atlas and the Kaplan-Meier Plotter platform. Furthermore, ITIH5 protein expression was evaluated by immunohistochemistry utilizing a tissue microarray with 385 distinct lung tissue samples. Based on our tissue collections, <i>ITIH5</i> mRNA expression was significantly decreased in NSCLC compared to normal lung tissue in line with an increased methylation frequency in lung cancer tissue. Independent TCGA data confirmed significant expression loss of <i>ITIH5</i> in lung cancer concordant with <i>ITIH5</i> promoter hypermethylation in NSCLC. Of interest, low <i>ITIH5</i> mRNA expression was particularly found in the magnoid and squamoid ADC expression subtype, concordant with an unfavorable patients' outcome in squamoid as well as tobacco smoking ADC patients. In conclusion, <i>ITIH5</i> may be a novel putative tumor suppressor gene in NSCLC with a potential molecular significance in the squamoid ADC subtype and further clinical impact for risk stratification of adenocarcinoma patients. In addition, <i>ITIH5</i> may serve as a novel biomarker for prognosis of tobacco smoking ADC patients.
DOI: 10.6084/m9.figshare.1568658.v1
title: The stability of Tenax TA thermal desorption tubes in simulated field conditions on the HAPSITE<sup>®</sup> ER

description: Due to the growing need to monitor aircraft cabin, cockpit and breathing-line air quality, functional assessment of sampling equipment for the specialised field conditions of flight need to be established for both in-flight and ground safety. In this article, we assess the reliability of Tenax TA thermal desorption tubes to perform under various relevant field sampling conditions, such as storage temperature, loading temperature, vibrational velocity, gravitational force (G Force) and altitude pressure with semi-real-time gas chromatograph-mass spectrometer (GC-MS) analysis on the field portable HAPSITE<sup>®</sup> ER (Hazardous Air Pollutants on Site Extended Range) instrument. First, we show that Tenax TA thermal desorption tubes can handle storage under extreme environmental conditions, 4–77°C, over numerous analytical test cycles. Next, we confirm that extreme loading temperature, both hot (77°C) and cold (4°C), does not affect the analytical reliability of Tenax TA thermal desorption tubes. Then, we illustrate that G Force may have a significant (<i>p</i> ≤ 0.0364) effect on Tenax TA performance while vibrational velocity (<i>p</i> ≤ 0.7265) and low ambient air pressure (<i>p</i> ≤ 0.1753), such as that found at high altitude, do not. Finally, several Tenax TA thermal desorption tubes were flight-tested, demonstrating that the durability of these tubes maybe insufficient for use on military cargo aircraft (<i>p</i> = 0.0107). The results presented here provide a rationale for additional testing of Tenax TA thermal desorption tubes for flight suitability.
DOI: 10.6084/m9.figshare.1568658.v3
title: The stability of Tenax TA thermal desorption tubes in simulated field conditions on the HAPSITE<sup>®</sup> ER

description: Due to the growing need to monitor aircraft cabin, cockpit and breathing-line air quality, functional assessment of sampling equipment for the specialised field conditions of flight need to be established for both in-flight and ground safety. In this article, we assess the reliability of Tenax TA thermal desorption tubes to perform under various relevant field sampling conditions, such as storage temperature, loading temperature, vibrational velocity, gravitational force (G Force) and altitude pressure with semi-real-time gas chromatograph-mass spectrometer (GC-MS) analysis on the field portable HAPSITE<sup>®</sup> ER (Hazardous Air Pollutants on Site Extended Range) instrument. First, we show that Tenax TA thermal desorption tubes can handle storage under extreme environmental conditions, 4–77°C, over numerous analytical test cycles. Next, we confirm that extreme loading temperature, both hot (77°C) and cold (4°C), does not affect the analytical reliability of Tenax TA thermal desorption tubes. Then, we illustrate that G Force may have a significant (<i>p</i> ≤ 0.0364) effect on Tenax TA performance while vibrational velocity (<i>p</i> ≤ 0.7265) and low ambient air pressure (<i>p</i> ≤ 0.1753), such as that found at high altitude, do not. Finally, several Tenax TA thermal desorption tubes were flight-tested, demonstrating that the durability of these tubes maybe insufficient for use on military cargo aircraft (<i>p</i> = 0.0107). The results presented here provide a rationale for additional testing of Tenax TA thermal desorption tubes for flight suitability.
DOI: 10.6084/m9.figshare.1568657.v2
title: Low expression of ITIH5 in adenocarcinoma of the lung is associated with unfavorable patients' outcome

description: Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is supposed to be involved in extracellular matrix stability and thus may play a key role in the inhibition of tumor progression. The current study is the first to analyze in depth ITIH5 expression and DNA methylation, as well as its potential clinical impact in non-small-cell lung carcinoma (NSCLC). We examined <i>ITIH5</i> mRNA expression in tumor and adjacent normal lung tissue specimens of NSCLC patients. In addition, methylation frequency of the <i>ITIH5</i> promoter was investigated using methylation-specific PCR and pyrosequencing. Significance of our data was validated by independent data sets from The Cancer Genome Atlas and the Kaplan-Meier Plotter platform. Furthermore, ITIH5 protein expression was evaluated by immunohistochemistry utilizing a tissue microarray with 385 distinct lung tissue samples. Based on our tissue collections, <i>ITIH5</i> mRNA expression was significantly decreased in NSCLC compared to normal lung tissue in line with an increased methylation frequency in lung cancer tissue. Independent TCGA data confirmed significant expression loss of <i>ITIH5</i> in lung cancer concordant with <i>ITIH5</i> promoter hypermethylation in NSCLC. Of interest, low <i>ITIH5</i> mRNA expression was particularly found in the magnoid and squamoid ADC expression subtype, concordant with an unfavorable patients' outcome in squamoid as well as tobacco smoking ADC patients. In conclusion, <i>ITIH5</i> may be a novel putative tumor suppressor gene in NSCLC with a potential molecular significance in the squamoid ADC subtype and further clinical impact for risk stratification of adenocarcinoma patients. In addition, <i>ITIH5</i> may serve as a novel biomarker for prognosis of tobacco smoking ADC patients.
DOI: 10.6084/m9.figshare.1568658.v2
title: The stability of Tenax TA thermal desorption tubes in simulated field conditions on the HAPSITE<sup>®</sup> ER

description: Due to the growing need to monitor aircraft cabin, cockpit and breathing-line air quality, functional assessment of sampling equipment for the specialised field conditions of flight need to be established for both in-flight and ground safety. In this article, we assess the reliability of Tenax TA thermal desorption tubes to perform under various relevant field sampling conditions, such as storage temperature, loading temperature, vibrational velocity, gravitational force (G Force) and altitude pressure with semi-real-time gas chromatograph-mass spectrometer (GC-MS) analysis on the field portable HAPSITE<sup>®</sup> ER (Hazardous Air Pollutants on Site Extended Range) instrument. First, we show that Tenax TA thermal desorption tubes can handle storage under extreme environmental conditions, 4–77°C, over numerous analytical test cycles. Next, we confirm that extreme loading temperature, both hot (77°C) and cold (4°C), does not affect the analytical reliability of Tenax TA thermal desorption tubes. Then, we illustrate that G Force may have a significant (<i>p</i> ≤ 0.0364) effect on Tenax TA performance while vibrational velocity (<i>p</i> ≤ 0.7265) and low ambient air pressure (<i>p</i> ≤ 0.1753), such as that found at high altitude, do not. Finally, several Tenax TA thermal desorption tubes were flight-tested, demonstrating that the durability of these tubes maybe insufficient for use on military cargo aircraft (<i>p</i> = 0.0107). The results presented here provide a rationale for additional testing of Tenax TA thermal desorption tubes for flight suitability.
DOI: 10.6084/m9.figshare.1568657.v3
title: Low expression of <i>ITIH5</i> in adenocarcinoma of the lung is associated with unfavorable patients' outcome

description: Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is supposed to be involved in extracellular matrix stability and thus may play a key role in the inhibition of tumor progression. The current study is the first to analyze in depth ITIH5 expression and DNA methylation, as well as its potential clinical impact in non-small-cell lung carcinoma (NSCLC). We examined <i>ITIH5</i> mRNA expression in tumor and adjacent normal lung tissue specimens of NSCLC patients. In addition, methylation frequency of the <i>ITIH5</i> promoter was investigated using methylation-specific PCR and pyrosequencing. Significance of our data was validated by independent data sets from The Cancer Genome Atlas and the Kaplan-Meier Plotter platform. Furthermore, ITIH5 protein expression was evaluated by immunohistochemistry utilizing a tissue microarray with 385 distinct lung tissue samples. Based on our tissue collections, <i>ITIH5</i> mRNA expression was significantly decreased in NSCLC compared to normal lung tissue in line with an increased methylation frequency in lung cancer tissue. Independent TCGA data confirmed significant expression loss of <i>ITIH5</i> in lung cancer concordant with <i>ITIH5</i> promoter hypermethylation in NSCLC. Of interest, low <i>ITIH5</i> mRNA expression was particularly found in the magnoid and squamoid ADC expression subtype, concordant with an unfavorable patients' outcome in squamoid as well as tobacco smoking ADC patients. In conclusion, <i>ITIH5</i> may be a novel putative tumor suppressor gene in NSCLC with a potential molecular significance in the squamoid ADC subtype and further clinical impact for risk stratification of adenocarcinoma patients. In addition, <i>ITIH5</i> may serve as a novel biomarker for prognosis of tobacco smoking ADC patients.
DOI: 10.6084/m9.figshare.1568657.v4
title: Low expression of <i>ITIH5</i> in adenocarcinoma of the lung is associated with unfavorable patients' outcome

description: Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is supposed to be involved in extracellular matrix stability and thus may play a key role in the inhibition of tumor progression. The current study is the first to analyze in depth ITIH5 expression and DNA methylation, as well as its potential clinical impact in non-small-cell lung carcinoma (NSCLC). We examined <i>ITIH5</i> mRNA expression in tumor and adjacent normal lung tissue specimens of NSCLC patients. In addition, methylation frequency of the <i>ITIH5</i> promoter was investigated using methylation-specific PCR and pyrosequencing. Significance of our data was validated by independent data sets from The Cancer Genome Atlas and the Kaplan-Meier Plotter platform. Furthermore, ITIH5 protein expression was evaluated by immunohistochemistry utilizing a tissue microarray with 385 distinct lung tissue samples. Based on our tissue collections, <i>ITIH5</i> mRNA expression was significantly decreased in NSCLC compared to normal lung tissue in line with an increased methylation frequency in lung cancer tissue. Independent TCGA data confirmed significant expression loss of <i>ITIH5</i> in lung cancer concordant with <i>ITIH5</i> promoter hypermethylation in NSCLC. Of interest, low <i>ITIH5</i> mRNA expression was particularly found in the magnoid and squamoid ADC expression subtype, concordant with an unfavorable patients' outcome in squamoid as well as tobacco smoking ADC patients. In conclusion, <i>ITIH5</i> may be a novel putative tumor suppressor gene in NSCLC with a potential molecular significance in the squamoid ADC subtype and further clinical impact for risk stratification of adenocarcinoma patients. In addition, <i>ITIH5</i> may serve as a novel biomarker for prognosis of tobacco smoking ADC patients.
DOI: 10.6084/m9.figshare.1568657.v6
title: Low expression of ITIH5 in adenocarcinoma of the lung is associated with unfavorable patients' outcome

description: Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is supposed to be involved in extracellular matrix stability and thus may play a key role in the inhibition of tumor progression. The current study is the first to analyze in depth ITIH5 expression and DNA methylation, as well as its potential clinical impact in non-small-cell lung carcinoma (NSCLC). We examined <i>ITIH5</i> mRNA expression in tumor and adjacent normal lung tissue specimens of NSCLC patients. In addition, methylation frequency of the <i>ITIH5</i> promoter was investigated using methylation-specific PCR and pyrosequencing. Significance of our data was validated by independent data sets from The Cancer Genome Atlas and the Kaplan-Meier Plotter platform. Furthermore, ITIH5 protein expression was evaluated by immunohistochemistry utilizing a tissue microarray with 385 distinct lung tissue samples. Based on our tissue collections, <i>ITIH5</i> mRNA expression was significantly decreased in NSCLC compared to normal lung tissue in line with an increased methylation frequency in lung cancer tissue. Independent TCGA data confirmed significant expression loss of <i>ITIH5</i> in lung cancer concordant with <i>ITIH5</i> promoter hypermethylation in NSCLC. Of interest, low <i>ITIH5</i> mRNA expression was particularly found in the magnoid and squamoid ADC expression subtype, concordant with an unfavorable patients' outcome in squamoid as well as tobacco smoking ADC patients. In conclusion, <i>ITIH5</i> may be a novel putative tumor suppressor gene in NSCLC with a potential molecular significance in the squamoid ADC subtype and further clinical impact for risk stratification of adenocarcinoma patients. In addition, <i>ITIH5</i> may serve as a novel biomarker for prognosis of tobacco smoking ADC patients.
DOI: 10.6084/m9.figshare.1568657.v5
title: Low expression of ITIH5 in adenocarcinoma of the lung is associated with unfavorable patients' outcome

description: Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is supposed to be involved in extracellular matrix stability and thus may play a key role in the inhibition of tumor progression. The current study is the first to analyze in depth ITIH5 expression and DNA methylation, as well as its potential clinical impact in non-small-cell lung carcinoma (NSCLC). We examined <i>ITIH5</i> mRNA expression in tumor and adjacent normal lung tissue specimens of NSCLC patients. In addition, methylation frequency of the <i>ITIH5</i> promoter was investigated using methylation-specific PCR and pyrosequencing. Significance of our data was validated by independent data sets from The Cancer Genome Atlas and the Kaplan-Meier Plotter platform. Furthermore, ITIH5 protein expression was evaluated by immunohistochemistry utilizing a tissue microarray with 385 distinct lung tissue samples. Based on our tissue collections, <i>ITIH5</i> mRNA expression was significantly decreased in NSCLC compared to normal lung tissue in line with an increased methylation frequency in lung cancer tissue. Independent TCGA data confirmed significant expression loss of <i>ITIH5</i> in lung cancer concordant with <i>ITIH5</i> promoter hypermethylation in NSCLC. Of interest, low <i>ITIH5</i> mRNA expression was particularly found in the magnoid and squamoid ADC expression subtype, concordant with an unfavorable patients' outcome in squamoid as well as tobacco smoking ADC patients. In conclusion, <i>ITIH5</i> may be a novel putative tumor suppressor gene in NSCLC with a potential molecular significance in the squamoid ADC subtype and further clinical impact for risk stratification of adenocarcinoma patients. In addition, <i>ITIH5</i> may serve as a novel biomarker for prognosis of tobacco smoking ADC patients.
DOI: 10.6084/m9.figshare.1568632
title: The meat of the matter: a rule of thumb for scavenging dogs?

description: Animals that scavenge in and around human settlements need to utilise a broad range of resources, and thus generalist scavengers are likely to be better adapted to human-dominated habitats. In India, free-ranging dogs (<i>Canis lupus familiaris</i>) live in close proximity with humans in diverse habitats, from forest fringes to metropolises, and are heavily dependent on humans for their food. It has been argued that the ability to digest carbohydrates was one of the driving forces for dog domestication. Though dogs are better adapted to digest carbohydrates than other canids, pet dogs show a clear preference for animal proteins. Our observations on streets of urban and semi-urban localities show that the free-ranging dogs are scavengers which primarily receive carbohydrate-rich food from humans. Their source for animal protein is typically garbage bins and leftovers, and such resources are rare. Using a series of field-based experiments, we test if the free-ranging dogs have adapted to a generalist scavenging lifestyle by losing preference for animal protein. Our experiments show that the free-ranging dogs, which are descendants of the decidedly carnivorous gray wolf (<i>Canis lupus lupus</i>), have retained a clear preference for meat, which is manifested by their choice of anything that smells of meat, irrespective of the actual nutrient content. The plasticity in their diet probably fosters efficient scavenging in a competitive environment, while a rule of thumb for preferentially acquiring specific nutrients enables them to sequester proteins from the carbohydrate-dominated environment.
DOI: 10.6084/m9.figshare.1568632.v2
title: The meat of the matter: a rule of thumb for scavenging dogs?

description: Animals that scavenge in and around human settlements need to utilise a broad range of resources, and thus generalist scavengers are likely to be better adapted to human-dominated habitats. In India, free-ranging dogs (<i>Canis lupus familiaris</i>) live in close proximity with humans in diverse habitats, from forest fringes to metropolises, and are heavily dependent on humans for their food. It has been argued that the ability to digest carbohydrates was one of the driving forces for dog domestication. Though dogs are better adapted to digest carbohydrates than other canids, pet dogs show a clear preference for animal proteins. Our observations on streets of urban and semi-urban localities show that the free-ranging dogs are scavengers which primarily receive carbohydrate-rich food from humans. Their source for animal protein is typically garbage bins and leftovers, and such resources are rare. Using a series of field-based experiments, we test if the free-ranging dogs have adapted to a generalist scavenging lifestyle by losing preference for animal protein. Our experiments show that the free-ranging dogs, which are descendants of the decidedly carnivorous gray wolf (<i>Canis lupus lupus</i>), have retained a clear preference for meat, which is manifested by their choice of anything that smells of meat, irrespective of the actual nutrient content. The plasticity in their diet probably fosters efficient scavenging in a competitive environment, while a rule of thumb for preferentially acquiring specific nutrients enables them to sequester proteins from the carbohydrate-dominated environment.
DOI: 10.6084/m9.figshare.1568632.v1
title: The meat of the matter: a rule of thumb for scavenging dogs?

description: Animals that scavenge in and around human settlements need to utilise a broad range of resources, and thus generalist scavengers are likely to be better adapted to human-dominated habitats. In India, free-ranging dogs (<i>Canis lupus familiaris</i>) live in close proximity with humans in diverse habitats, from forest fringes to metropolises, and are heavily dependent on humans for their food. It has been argued that the ability to digest carbohydrates was one of the driving forces for dog domestication. Though dogs are better adapted to digest carbohydrates than other canids, pet dogs show a clear preference for animal proteins. Our observations on streets of urban and semi-urban localities show that the free-ranging dogs are scavengers which primarily receive carbohydrate-rich food from humans. Their source for animal protein is typically garbage bins and leftovers, and such resources are rare. Using a series of field-based experiments, we test if the free-ranging dogs have adapted to a generalist scavenging lifestyle by losing preference for animal protein. Our experiments show that the free-ranging dogs, which are descendants of the decidedly carnivorous gray wolf (<i>Canis lupus lupus</i>), have retained a clear preference for meat, which is manifested by their choice of anything that smells of meat, irrespective of the actual nutrient content. The plasticity in their diet probably fosters efficient scavenging in a competitive environment, while a rule of thumb for preferentially acquiring specific nutrients enables them to sequester proteins from the carbohydrate-dominated environment.
DOI: 10.6084/m9.figshare.1568612
title: Economic resources consumption structure in severe hypoglycemia episodes: a systematic review and meta-analysis

description: Diabetes mellitus (DM) is associated with severe hypoglycemia events (SHEs) that vary in severity and resource consumption. Here we perform a systematic review in Medline of studies evaluating SHE-related health resource use. Eligible studies investigated patients with DM and included ≥10 SHEs. We also assessed studies identified in another systematic review, and through references from the included studies. We identified 14 relevant studies and used data from 11 (encompassing 6075 patients). Study results were interpreted to fit our definitions, which sometimes required assumptions. SHE type structure was synthesized using Bayesian modeling. Estimating Type 1 & 2 DM separately revealed only small differences; therefore, we used joint results. Of the analyzed SHEs, 9.97% were hospital-treated, 22.3% medical professional-treated, and 67.73% family-treated. These meta-analysis results help in understanding the structure of resource consumption following SHE and can be used in economic studies.