DOI: 10.5061/dryad.7dk08bc
title: Data from: Stabilization of primary cilia reduces abortive cell cyle re-entry to protect injured adult CNS neurons from apoptosis

description: Abortive cell cycle (ACC) re-entry of apoptotic neurons is a recently
 characterized phenomenon that occurs after central nervous system (CNS)
 injury or over the course of CNS disease. Consequently, inhibiting cell
 cycle progression is neuroprotective in numerous CNS pathology models.
 Primary cilia are ubiquitous, centriole-based cellular organelles that
 prevent cell cycling, but their ability to modulate abortive cell cycle
 has not been described. Here, we show that neuronal cilia are ablated
 in-vitro and in-vivo following injury by hypoxia or optic nerve
 transection (ONT), respectively. Furthermore, forced cilia resorption
 sensitized neurons to these injuries and enhanced cell death. In contrast,
 pharmacological inhibition or shRNA knockdown of the proteins that
 disassemble the cilia increased neuron survival and decreased the
 phosphorylation of retinoblastoma (Rb), a master switch for cell cycle
 re-entry. Our findings show that the stabilization of neuronal primary
 cilia inhibits apoptotic cell cycling, which has implications for future
 therapeutic strategies that halt or slow the progression of
 neurodegenerative diseases and acute CNS injuries.
description: WST-8 measurement of
 N1E-115 activity after 5 hours of hypoxia and 5 hours of hypoxia+24 hours
 of normoxiaThis file contains is
 the data for Fig 1C. It includes the raw Wst-8 measurements of N1E-115
 cells after 5 hours of hypoxia and 5 hours of Hypoxia+24 hours of normoxia
 and these measurements normalized to 100.Fig
 1.xlsxN1E-115 survival after
 Ciliobrevin TreatmentThis file contains the
 WST8 measurements for N1E-115 survival after Ciliobrevin D treatment
 (sheet B) and N1E-115 survival after hypoxia and Ciliobrevin D treatment
 (sheet D).Fig
 2.xlsxAraC purification of
 cortical neuron culture and neuron survival after Tubastatin A and Aurora
 A Inhibitor I treatmentThis file contains the
 data for fig 3. Sheet C contains the counts for cortical neurons (NeuN)
 and GFAP in AraC treated and non-treated cultures. Sheet E contains the
 data for the survival counts of Neurons injured by etoposide but treated
 with Tubastatin A in combination with or without AraC. Sheet F Contains
 the survival counts of neurons injured by etoposide but treated with
 Aurora A inhibitor.Fig
 3.xlsxMeasurements of primary
 ciliaThis file contains the
 file 6 which includes the measurements of retinal ganglion cell primary
 cilia length and the counts of their nuclei.Fig
 6.xlsxIFT88 shRNA effects on
 neuron primary cilia length and survival after axotomyThis file contains the
 data for fig 7. Sheet B,C contains the measurements of retinal ganglion
 cell primary cilia lengths after treatment with control or IFT88 shRNA.
 Sheet E contains survival counts of IFT88 treated RGCs after
 axotomy.Fig
 7.xlsxThe effects of various
 inhibitors and shRNAs targeting ciliary resorption on neuronal survival
 after axotomyThis file contains is
 the data for Fig 8. Sheet B contains the axotomized retinal ganglion cell
 survival quantification after treatment with Tubastatin A or Aurora
 Inhibitor A. Sheet D contains the axotomized retinal ganglion cell
 survival quantification after treatment with HEF1, NDE1, PLK1, Kif2a
 shRNAs.Fig
 8.xlsx