DOI: 10.5061/dryad.s4mw6m9c8
title: Data from: Sex-specific developmental alterations in DYRK1A expression in the brain of a Down syndrome mouse model

description: Aberrant neurodevelopment in Down syndrome (DS)-caused by triplication of
 human chromosome 21-is commonly attributed to gene dosage imbalance,
 linking overexpression of trisomic genes with disrupted developmental
 processes, with DYRK1A particularly implicated. We hypothesized that
 regional brain DYRK1A protein overexpression in trisomic mice varies over
 development in sex-specific patterns that may be distinct from Dyrk1a
 transcription, and reduction of Dyrk1a copy number from 3 to 2 in
 otherwise trisomic mice reduces DYRK1A, independent of other trisomic
 genes. DYRK1A overexpression varied with age, sex, and brain region, with
 peak overexpression on postnatal day (P) 6 in both sexes. Sex-dependent
 differences were also evident from P15-P24. Reducing Dyrk1a copy number
 confirmed that these differences depended on Dyrk1a gene dosage and not
 other trisomic genes. Trisomic Dyrk1a mRNA and protein expression were not
 highly correlated. Sex-specific patterns of DYRK1A overexpression during
 trisomic neurodevelopment may provide mechanistic targets for therapeutic
 intervention in DS.
description: # Data from: Sex-specific developmental alterations in DYRK1A expression
 in the brain of a Down syndrome mouse model Hawley, L.E., M.E. Stringer,
 A. Parker, A. Folz, C.R. Goodlett, R.J. Roper. Sex-specific developmental
 alterations in DYRK1A expression in the brain of a Down syndrome mouse
 model. Science This README file was generated on 2024-01-10 by Laura
 Hawley. GENERAL INFORMATION 1\. Title of Dataset: Data from: Sex-specific
 developmental alterations in DYRK1A expression in the brain of a Down
 syndrome mouse model 2\. Author Information A. Principal Investigator
 Contact Information Name: Randall Roper Institution: Indiana University
 Purdue University Indianapolis Address: Indianapolis, IN USA Email:
 rjroper@iu.edu 3\. Date of data collection (single date, range,
 approximate date): January 2027 – June 2022 4\. Geographic location of
 data collection: Indianapolis, Indiana 5\. Information about funding
 sources that supported the collection of the data: Beaty/C-Tech Fund –
 Blue River Community Foundation; Indiana Clinical and Translational
 Sciences Institute; National Institutes of Health, National Center for
 Advancing Translational Sciences, Clinical and Translational Sciences
 Award, Grant number UL1TR001108; National Institutes of Health, National
 Institute of Arthritis and Musculoskeletal and Skin Diseases grant
 AR078663 to RJR and CRG. SHARING/ACCESS INFORMATION 1\.
 Licenses/restrictions placed on the data: CC0 1.0 Universal (CC0 1.0)
 Public Domain –licenses/restriction numbers 2\. Links to publications that
 cite or use the data: Hawley, L.E., Prochaska, F., Stringer, M., Goodlett,
 C.R., Roper, R.J. (2022). Sexually dimorphic DYRK1A overexpression on
 postnatal day 15 in the Ts65Dn mouse model of Down syndrome: Effects of
 pharmacological targeting on behavioral phenotypes. Pharmacology,
 Biochemistry and Behavior. 3\. Links to other publicly accessible
 locations of the data: None 4\. Links/relationships to ancillary data
 sets: None 5\. Was data derived from another source? No A. If yes, list
 source(s): NA 6\. Recommended citation for this dataset: Hawley, L.E.,
 Prochaska, F., Stringer, M., Goodlett, C.R., Roper, R.J. (2022). Data
 from: Sexually dimorphic DYRK1A overexpression on postnatal day 15 in the
 Ts65Dn mouse model of Down syndrome: Effects of pharmacological targeting
 on behavioral phenotypes. Dryad Digital Repositry. https://doi.org/link
 specifics This zip directory contains the data and information required to
 reproduce analyses reported in the associated paper. Contact: Randall J.
 Roper ([rjroper@iu.edu](mailto:rjroper@iu.edu)) Folder:
 Hawley_2023_WesternBlot_ByAge\ This folder contains results from Western
 blot analysis of DYRK1A in brain tissues from Ts65Dn mice at different
 ages of development.Files:\ Hawley_2023_WBData_E18.5.csv\
 Hawley_2023_WBData_P0.csv\ Hawley_2023_WBData_P03.csv\
 Hawley_2023_WBData_P06.csv\ Hawley_2023_WBData_P12.csv\
 Hawley_2023_WBData_P15.csv\ Hawley_2023_WBData_P18.csv\
 Hawley_2023_WBData_P21.csv\ Hawley_2023_WBData_P24.csv Folder:
 Hawley_2023_WesternBlot_Knockdown_ByAge\ This folder contains results from
 Western blot analysis of DYRK1A in brain tissues from the Ts65Dn
 mice.Dyrk1a+/- knockdown model on postnatal days 6 and 15.\ Files:\
 Hawley_2023_WBData_KnckDwn_P06.csv\ Hawley_2023_WBData_KnckDwn_P15.csv
 Folder: Hawley_2023_RTqPCR \_ByAge\ This folder contains results from
 RT-qPCR analysis of Dyrk1a mRNA transcripts in brain tissues from Ts65Dn
 mice at postnatal days 0, 3, and 6.\ Files:\ Hawley_2023_RTqPCR_P0.csv\
 Hawley_2023_RTqPCR_P3.csv\ Hawley_2023_RTqPCR_P6.csv Folder:
 Hawley_2023_RTqPCR_Knockdown_ByAge\ This folder contains results from
 RT-qPCR analysis of Dyrk1a mRNA transcripts in brain tissues from the
 Ts65Dn mice.Dyrk1a+/- knockdown model on postnatal day 6.File:\
 Hawley_2023_RTqPCR_KnckDwn_P06.csv Abbreviations used: E-embryonic day;
 P-postnatal day; WB-Western blot; KnckDwn-Knockdown Note: The final three
 rows in file Hawley_2023_WBData_P06.csv contain results from a single
 subject that was run on two separate Western blots in error. Row
 "5131" contains the results from the first analysis, row
 "5132" contains the results from the second. Row
 "5133" is the average of these two analyses and was used for
 calculations in this project. DATA & FILE OVERVIEW 1\. File List: A)
 Hawley_2023_WBData_E18.5.xlsx B) Hawley_2023_WBData_P0.xlsx C)
 Hawley_2023_WBData_P03.xlsx D) Hawley_2023_WBData_P06.xlsx E)
 Hawley_2023_WBData_P12.xlsx F) Hawley_2023_WBData_P15.xlsx G)
 Hawley_2023_WBData_P18.xlsx H) Hawley_2023_WBData_P21.xlsx I)
 Hawley_2023_WBData_P24.xlsx J) Hawley_2023_WBData_KnckDwn_P06.xlsx K)
 Hawley_2023_WBData_KnckDwn_P15.xlsx L) Hawley_2023_RTqPCR_P0.xlsx M)
 Hawley_2023_RTqPCR_P3.xlsx N) Hawley_2023_RTqPCR_P6.xlsx O)
 Hawley_2023_RTqPCR_KnckDwn_P06.xlsx 2\. Relationship between files, if
 important: None 3\. Additional related data collected that was not
 included in the current data package: None 4\. Are there multiple versions
 of the dataset? No A. If yes, name of file(s) that was updated: NA i. Why
 was the file updated? NA ii. When was the file updated? NA
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_E18.5.xlsx 1\. Number of
 variables: 12 2\. Number of cases/rows: 29 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number: Western blot identification number on which the subject’s whole
 brain tissue sample was analyzed \* Age: embryonic age/number of days post
 conception of the subject at tissue collection \* Eu=1, Ts=2: genotype
 (euploid/control = 1; Ts65Dn/trisomic = 2) \* F=1, M=2: sex (female = 1;
 male = 2) \* D1A value-WholeB: optical density value of DYRK1A obtained
 from the subject’s whole brain tissue sample \* TolProt value-WholeB:
 numeric value of Total Protein obtained from the subject’s whole brain
 tissue sample \* Normalized Sample D1A/TotProt: normalized value of DYRK1A
 optical density divided by the numeric value of total protein within the
 subject’s whole brain tissue sample \* Blot Mean of Normalized Euploid
 Samples: average of the normalized DYRK1A values from all euploid
 subjects’ whole brain tissue samples on the blot \* Relative Ratio (Norm
 Sample/Blot Mean of Norm Eu): relative ratio of the normalized DYRK1A
 value from the subject’s whole brain tissue sample compared to the average
 normalized DYRK1A value of all euploid subjects’ whole brain tissue
 samples on the blot \* Z-Score WholeB: Standard scores of the subject’s
 whole brain DYRK1A relative intensity, obtained by converting each
 relative intensity value to a standard score corresponding to the number
 of standard deviation units above or below the mean of the comparable
 euploid group \* % of Control WholeB: relative ratio of DYRK1A in the
 subject’s whole brain tissue sample converted to a percentage 4\. Missing
 data codes: NA (data not available); "." or "x"
 indicate missing data for an individual 5\. Specialized formats or other
 abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P0.xlsx 1\. Number of
 variables: 20 2\. Number of cases/rows: 32 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number FB: Western blot identification number on which the subject’s
 forebrain tissue sample was analyzed \* Age: postnatal age of the subject
 at tissue collection \* Eu=1, Ts=2: genotype (euploid/control = 1;
 Ts65Dn/trisomic = 2) \* F=1, M=2: sex (female = 1; male = 2) \* D1A
 value-FB: optical density value of DYRK1A obtained from the subject’s
 forebrain tissue sample \* TolProt value-FB: numeric value of Total
 Protein obtained from the subject’s forebrain tissue sample \* Normalized
 Sample FB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s forebrain
 tissue sample \* Blot Mean of FB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ forebrain tissue
 samples on the blot \* Relative Ratio FB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 forebrain tissue sample compared to the average normalized DYRK1A value of
 all euploid subjects’ forebrain tissue samples on the blot \* Z-Score FB:
 Standard scores of the subject’s forebrain DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control FB: relative ratio of
 DYRK1A in the subject’s forebrain tissue sample converted to a percentage
 *Blot Number CB: Western blot identification number on which the subject’s
 cerebellar tissue sample was analyzed \* D1A value-CB: optical density
 value of DYRK1A obtained from the subject’s cerebellar tissue sample \*
 TolProt value-CB: numeric value of Total Protein obtained from the
 subject’s cerebellar tissue sample \* Normalized Sample CB D1A/TotProt:
 normalized value of DYRK1A optical density divided by the numeric value of
 total protein within the subject’s cerebellar tissue sample \* Blot Mean
 of CB Normalized Euploid Samples: average of the normalized DYRK1A values
 from all euploid subjects’ cerebellar tissue samples on the blot \*
 Relative Ratio CB (Norm Sample/Blot Mean of Norm Eu): relative ratio of
 the normalized DYRK1A value from the subject’s cerebellar tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 cerebellar tissue samples on the blot \* Z-Score CB: Standard scores of
 the subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CB: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage 4\.
 Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P03.xlsx 1\. Number of
 variables: 19 2\. Number of cases/rows: 32 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number FB: Western blot identification number on which the subject’s
 forebrain tissue sample was analyzed \* Age: postnatal age of the subject
 at tissue collection \* Eu=1, Ts=2: genotype (euploid/control = 1;
 Ts65Dn/trisomic = 2) \* F=1, M=2: sex (female = 1; male = 2) \* D1A
 value-FB: optical density value of DYRK1A obtained from the subject’s
 forebrain tissue sample \* TolProt value-FB: numeric value of Total
 Protein obtained from the subject’s forebrain tissue sample \* Normalized
 Sample FB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s forebrain
 tissue sample \* Blot Mean of FB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ forebrain tissue
 samples on the blot \* Relative Ratio FB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 forebrain tissue sample compared to the average normalized DYRK1A value of
 all euploid subjects’ forebrain tissue samples on the blot \* Z-Score FB:
 Standard scores of the subject’s forebrain DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control FB: relative ratio of
 DYRK1A in the subject’s forebrain tissue sample converted to a percentage
 \* D1A value-CB: optical density value of DYRK1A obtained from the
 subject’s cerebellar tissue sample \* TolProt value-CB: numeric value of
 Total Protein obtained from the subject’s cerebellar tissue sample \*
 Normalized Sample CB D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebellar tissue sample \* Blot Mean of CB Normalized Euploid Samples:
 average of the normalized DYRK1A values from all euploid subjects’
 cerebellar tissue samples on the blot \* Relative Ratio CB (Norm
 Sample/Blot Mean of Norm Eu): relative ratio of the normalized DYRK1A
 value from the subject’s cerebellar tissue sample compared to the average
 normalized DYRK1A value of all euploid subjects’ cerebellar tissue samples
 on the blot \* Z-Score CB: Standard scores of the subject’s cerebellar
 DYRK1A relative intensity, obtained by converting each relative intensity
 value to a standard score corresponding to the number of standard
 deviation units above or below the mean of the comparable euploid group \*
 % of Control CB: relative ratio of DYRK1A in the subject’s cerebellar
 tissue sample converted to a percentage 4\. Missing data codes: NA (data
 not available); "." or "x" indicate missing data for
 an individual 5\. Specialized formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P06.xlsx 1\. Number of
 variables: 26 2\. Number of cases/rows: 34* The final three rows in file
 Hawley_2023_WBData_P06.xlsx contain results from a single subject that was
 run on two separate Western blots in error. Row "513.1" contains
 the results from the first analysis, row "513.2" contains the
 results from the second. Row "513.3" is the average of these two
 analyses and was used for calculations in this project. 3\. Variable List:
 \* Animal number: unique identification number assigned to the subject \*
 Blot number: Western blot identification number on which the subject’s
 samples were analyzed \* Age: postnatal age of the subject at tissue
 collection \* Eu=1, Ts=2: genotype (euploid/control = 1; Ts65Dn/trisomic =
 2) \* F=1, M=2: sex (female = 1; male = 2) \* D1A value-HIP: optical
 density value of DYRK1A obtained from the subject’s hippocampal tissue
 sample \* TolProt value-HIP: numeric value of Total Protein obtained from
 the subject’s hippocampal tissue sample \* Normalized Sample HIP
 D1A/TotProt: normalized value of DYRK1A optical density divided by the
 numeric value of total protein within the subject’s hippocampal tissue
 sample \* Blot Mean of HIP Normalized Euploid Samples: average of the
 normalized DYRK1A values from all euploid subjects’ hippocampal tissue
 samples on the blot \* Relative Ratio HIP (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 hippocampal tissue sample compared to the average normalized DYRK1A value
 of all euploid subjects’ hippocampal tissue samples on the blot \* Z-Score
 HIP: Standard scores of the subject’s hippocampal DYRK1A relative
 intensity, obtained by converting each relative intensity value to a
 standard score corresponding to the number of standard deviation units
 above or below the mean of the comparable euploid group \* % of
 Control-HIP: relative ratio of DYRK1A in the subject’s hippocampal tissue
 sample converted to a percentage \* D1A value-CTX: optical density value
 of DYRK1A obtained from the subject’s cerebral cortex tissue sample \*
 TolProt value-CTX: numeric value of Total Protein obtained from the
 subject’s cerebral cortex tissue sample \* Normalized Sample CTX
 D1A/TotProt: normalized value of DYRK1A optical density divided by the
 numeric value of total protein within the subject’s cerebral cortex tissue
 sample \* Blot Mean of CTX Normalized Euploid Samples: average of the
 normalized DYRK1A values from all euploid subjects’ cerebral cortex tissue
 samples on the blot \* Relative Ratio CTX (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 cerebral cortex tissue sample compared to the average normalized DYRK1A
 value of all euploid subjects’ cerebral cortex tissue samples on the blot
 \* Z-Score CTX: Standard scores of the subject’s cerebellar DYRK1A
 relative intensity, obtained by converting each relative intensity value
 to a standard score corresponding to the number of standard deviation
 units above or below the mean of the comparable euploid group \* % of
 Control CTX: relative ratio of DYRK1A in the subject’s cerebellar tissue
 sample converted to a percentage \* D1A value-CB: optical density value of
 DYRK1A obtained from the subject’s cerebellar tissue sample \* TolProt
 value-CB: numeric value of Total Protein obtained from the subject’s
 cerebellar tissue sample \* Normalized Sample CB D1A/TotProt: normalized
 value of DYRK1A optical density divided by the numeric value of total
 protein within the subject’s cerebellar tissue sample \* Blot Mean of CB
 Normalized Euploid Samples: average of the normalized DYRK1A values from
 all euploid subjects’ cerebellar tissue samples on the blot \* Relative
 Ratio CB (Norm Sample/Blot Mean of Norm Eu): relative ratio of the
 normalized DYRK1A value from the subject’s cerebellar tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 cerebellar tissue samples on the blot \* Z-Score CB: Standard scores of
 the subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CB: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage 4\.
 Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P12.xlsx 1\. Number of
 variables: 26 2\. Number of cases/rows: 35 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number: Western blot identification number on which the subject’s samples
 were analyzed \* Age: postnatal age of the subject at tissue collection \*
 Eu=1, Ts=2: genotype (euploid/control = 1; Ts65Dn/trisomic = 2) \* F=1,
 M=2: sex (female = 1; male = 2) \* D1A value-HIP: optical density value of
 DYRK1A obtained from the subject’s hippocampal tissue sample \* TolProt
 value-HIP: numeric value of Total Protein obtained from the subject’s
 hippocampal tissue sample \* Normalized Sample HIP D1A/TotProt: normalized
 value of DYRK1A optical density divided by the numeric value of total
 protein within the subject’s hippocampal tissue sample \* Blot Mean of HIP
 Normalized Euploid Samples: average of the normalized DYRK1A values from
 all euploid subjects’ hippocampal tissue samples on the blot \* Relative
 Ratio HIP (Norm Sample/Blot Mean of Norm Eu): relative ratio of the
 normalized DYRK1A value from the subject’s hippocampal tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 hippocampal tissue samples on the blot \* Z-Score HIP: Standard scores of
 the subject’s hippocampal DYRK1A relative intensity, obtained by
 converting each relative intensity value to a standard score corresponding
 to the number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control-HIP: relative ratio of DYRK1A in
 the subject’s hippocampal tissue sample converted to a percentage \* D1A
 value-CTX: optical density value of DYRK1A obtained from the subject’s
 cerebral cortex tissue sample \* TolProt value-CTX: numeric value of Total
 Protein obtained from the subject’s cerebral cortex tissue sample \*
 Normalized Sample CTX D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebral cortex tissue sample \* Blot Mean of CTX Normalized Euploid
 Samples: average of the normalized DYRK1A values from all euploid
 subjects’ cerebral cortex tissue samples on the blot \* Relative Ratio CTX
 (Norm Sample/Blot Mean of Norm Eu): relative ratio of the normalized
 DYRK1A value from the subject’s cerebral cortex tissue sample compared to
 the average normalized DYRK1A value of all euploid subjects’ cerebral
 cortex tissue samples on the blot \* Z-Score CTX: Standard scores of the
 subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CTX: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage \* D1A
 value-CB: optical density value of DYRK1A obtained from the subject’s
 cerebellar tissue sample \* TolProt value-CB: numeric value of Total
 Protein obtained from the subject’s cerebellar tissue sample \* Normalized
 Sample CB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s cerebellar
 tissue sample \* Blot Mean of CB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ cerebellar tissue
 samples on the blot \* Relative Ratio CB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 cerebellar tissue sample compared to the average normalized DYRK1A value
 of all euploid subjects’ cerebellar tissue samples on the blot \* Z-Score
 CB: Standard scores of the subject’s cerebellar DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control CB: relative ratio of
 DYRK1A in the subject’s cerebellar tissue sample converted to a percentage
 4\. Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P15.xlsx 1\. Number of
 variables: 26 2\. Number of cases/rows: 33 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number: Western blot identification number on which the subject’s samples
 were analyzed \* Age: postnatal age of the subject at tissue collection \*
 Eu=1, Ts=2: genotype (euploid/control = 1; Ts65Dn/trisomic = 2) \* F=1,
 M=2: sex (female = 1; male = 2) \* D1A value-HIP: optical density value of
 DYRK1A obtained from the subject’s hippocampal tissue sample \* TolProt
 value-HIP: numeric value of Total Protein obtained from the subject’s
 hippocampal tissue sample \* Normalized Sample HIP D1A/TotProt: normalized
 value of DYRK1A optical density divided by the numeric value of total
 protein within the subject’s hippocampal tissue sample \* Blot Mean of HIP
 Normalized Euploid Samples: average of the normalized DYRK1A values from
 all euploid subjects’ hippocampal tissue samples on the blot \* Relative
 Ratio HIP (Norm Sample/Blot Mean of Norm Eu): relative ratio of the
 normalized DYRK1A value from the subject’s hippocampal tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 hippocampal tissue samples on the blot \* Z-Score HIP: Standard scores of
 the subject’s hippocampal DYRK1A relative intensity, obtained by
 converting each relative intensity value to a standard score corresponding
 to the number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control-HIP: relative ratio of DYRK1A in
 the subject’s hippocampal tissue sample converted to a percentage \* D1A
 value-CTX: optical density value of DYRK1A obtained from the subject’s
 cerebral cortex tissue sample \* TolProt value-CTX: numeric value of Total
 Protein obtained from the subject’s cerebral cortex tissue sample \*
 Normalized Sample CTX D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebral cortex tissue sample \* Blot Mean of CTX Normalized Euploid
 Samples: average of the normalized DYRK1A values from all euploid
 subjects’ cerebral cortex tissue samples on the blot \* Relative Ratio CTX
 (Norm Sample/Blot Mean of Norm Eu): relative ratio of the normalized
 DYRK1A value from the subject’s cerebral cortex tissue sample compared to
 the average normalized DYRK1A value of all euploid subjects’ cerebral
 cortex tissue samples on the blot \* Z-Score CTX: Standard scores of the
 subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CTX: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage \* D1A
 value-CB: optical density value of DYRK1A obtained from the subject’s
 cerebellar tissue sample \* TolProt value-CB: numeric value of Total
 Protein obtained from the subject’s cerebellar tissue sample \* Normalized
 Sample CB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s cerebellar
 tissue sample \* Blot Mean of CB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ cerebellar tissue
 samples on the blot \* Relative Ratio CB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 cerebellar tissue sample compared to the average normalized DYRK1A value
 of all euploid subjects’ cerebellar tissue samples on the blot \* Z-Score
 CB: Standard scores of the subject’s cerebellar DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control CB: relative ratio of
 DYRK1A in the subject’s cerebellar tissue sample converted to a percentage
 4\. Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P18.xlsx 1\. Number of
 variables: 26 2\. Number of cases/rows: 32 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number: Western blot identification number on which the subject’s samples
 were analyzed \* Age: postnatal age of the subject at tissue collection \*
 Eu=1, Ts=2: genotype (euploid/control = 1; Ts65Dn/trisomic = 2) \* F=1,
 M=2: sex (female = 1; male = 2) \* D1A value-HIP: optical density value of
 DYRK1A obtained from the subject’s hippocampal tissue sample \* TolProt
 value-HIP: numeric value of Total Protein obtained from the subject’s
 hippocampal tissue sample \* Normalized Sample HIP D1A/TotProt: normalized
 value of DYRK1A optical density divided by the numeric value of total
 protein within the subject’s hippocampal tissue sample \* Blot Mean of HIP
 Normalized Euploid Samples: average of the normalized DYRK1A values from
 all euploid subjects’ hippocampal tissue samples on the blot \* Relative
 Ratio HIP (Norm Sample/Blot Mean of Norm Eu): relative ratio of the
 normalized DYRK1A value from the subject’s hippocampal tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 hippocampal tissue samples on the blot \* Z-Score HIP: Standard scores of
 the subject’s hippocampal DYRK1A relative intensity, obtained by
 converting each relative intensity value to a standard score corresponding
 to the number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control-HIP: relative ratio of DYRK1A in
 the subject’s hippocampal tissue sample converted to a percentage \* D1A
 value-CTX: optical density value of DYRK1A obtained from the subject’s
 cerebral cortex tissue sample \* TolProt value-CTX: numeric value of Total
 Protein obtained from the subject’s cerebral cortex tissue sample \*
 Normalized Sample CTX D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebral cortex tissue sample \* Blot Mean of CTX Normalized Euploid
 Samples: average of the normalized DYRK1A values from all euploid
 subjects’ cerebral cortex tissue samples on the blot \* Relative Ratio CTX
 (Norm Sample/Blot Mean of Norm Eu): relative ratio of the normalized
 DYRK1A value from the subject’s cerebral cortex tissue sample compared to
 the average normalized DYRK1A value of all euploid subjects’ cerebral
 cortex tissue samples on the blot \* Z-Score CTX: Standard scores of the
 subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CTX: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage \* D1A
 value-CB: optical density value of DYRK1A obtained from the subject’s
 cerebellar tissue sample \* TolProt value-CB: numeric value of Total
 Protein obtained from the subject’s cerebellar tissue sample \* Normalized
 Sample CB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s cerebellar
 tissue sample \* Blot Mean of CB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ cerebellar tissue
 samples on the blot \* Relative Ratio CB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 cerebellar tissue sample compared to the average normalized DYRK1A value
 of all euploid subjects’ cerebellar tissue samples on the blot \* Z-Score
 CB: Standard scores of the subject’s cerebellar DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control CB: relative ratio of
 DYRK1A in the subject’s cerebellar tissue sample converted to a percentage
 4\. Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P21.xlsx 1\. Number of
 variables: 26 2\. Number of cases/rows: 24 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number: Western blot identification number on which the subject’s samples
 were analyzed \* Age: postnatal age of the subject at tissue collection \*
 Eu=1, Ts=2: genotype (euploid/control = 1; Ts65Dn/trisomic = 2) \* F=1,
 M=2: sex (female = 1; male = 2) \* D1A value-HIP: optical density value of
 DYRK1A obtained from the subject’s hippocampal tissue sample \* TolProt
 value-HIP: numeric value of Total Protein obtained from the subject’s
 hippocampal tissue sample \* Normalized Sample HIP D1A/TotProt: normalized
 value of DYRK1A optical density divided by the numeric value of total
 protein within the subject’s hippocampal tissue sample \* Blot Mean of HIP
 Normalized Euploid Samples: average of the normalized DYRK1A values from
 all euploid subjects’ hippocampal tissue samples on the blot \* Relative
 Ratio HIP (Norm Sample/Blot Mean of Norm Eu): relative ratio of the
 normalized DYRK1A value from the subject’s hippocampal tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 hippocampal tissue samples on the blot \* Z-Score HIP: Standard scores of
 the subject’s hippocampal DYRK1A relative intensity, obtained by
 converting each relative intensity value to a standard score corresponding
 to the number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control-HIP: relative ratio of DYRK1A in
 the subject’s hippocampal tissue sample converted to a percentage \* D1A
 value-CTX: optical density value of DYRK1A obtained from the subject’s
 cerebral cortex tissue sample \* TolProt value-CTX: numeric value of Total
 Protein obtained from the subject’s cerebral cortex tissue sample \*
 Normalized Sample CTX D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebral cortex tissue sample \* Blot Mean of CTX Normalized Euploid
 Samples: average of the normalized DYRK1A values from all euploid
 subjects’ cerebral cortex tissue samples on the blot \* Relative Ratio CTX
 (Norm Sample/Blot Mean of Norm Eu): relative ratio of the normalized
 DYRK1A value from the subject’s cerebral cortex tissue sample compared to
 the average normalized DYRK1A value of all euploid subjects’ cerebral
 cortex tissue samples on the blot \* Z-Score CTX: Standard scores of the
 subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CTX: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage \* D1A
 value-CB: optical density value of DYRK1A obtained from the subject’s
 cerebellar tissue sample \* TolProt value-CB: numeric value of Total
 Protein obtained from the subject’s cerebellar tissue sample \* Normalized
 Sample CB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s cerebellar
 tissue sample \* Blot Mean of CB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ cerebellar tissue
 samples on the blot \* Relative Ratio CB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 cerebellar tissue sample compared to the average normalized DYRK1A value
 of all euploid subjects’ cerebellar tissue samples on the blot \* Z-Score
 CB: Standard scores of the subject’s cerebellar DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control CB: relative ratio of
 DYRK1A in the subject’s cerebellar tissue sample converted to a percentage
 4\. Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_P24.xlsx 1\. Number of
 variables: 26 2\. Number of cases/rows: 31 3\. Variable List: \* Animal
 number: unique identification number assigned to the subject \* Blot
 number: Western blot identification number on which the subject’s samples
 were analyzed \* Age: postnatal age of the subject at tissue collection \*
 Eu=1, Ts=2: genotype (euploid/control = 1; Ts65Dn/trisomic = 2) \* F=1,
 M=2: sex (female = 1; male = 2) \* D1A value-HIP: optical density value of
 DYRK1A obtained from the subject’s hippocampal tissue sample \* TolProt
 value-HIP: numeric value of Total Protein obtained from the subject’s
 hippocampal tissue sample \* Normalized Sample HIP D1A/TotProt: normalized
 value of DYRK1A optical density divided by the numeric value of total
 protein within the subject’s hippocampal tissue sample \* Blot Mean of HIP
 Normalized Euploid Samples: average of the normalized DYRK1A values from
 all euploid subjects’ hippocampal tissue samples on the blot \* Relative
 Ratio HIP (Norm Sample/Blot Mean of Norm Eu): relative ratio of the
 normalized DYRK1A value from the subject’s hippocampal tissue sample
 compared to the average normalized DYRK1A value of all euploid subjects’
 hippocampal tissue samples on the blot \* Z-Score HIP: Standard scores of
 the subject’s hippocampal DYRK1A relative intensity, obtained by
 converting each relative intensity value to a standard score corresponding
 to the number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control-HIP: relative ratio of DYRK1A in
 the subject’s hippocampal tissue sample converted to a percentage \* D1A
 value-CTX: optical density value of DYRK1A obtained from the subject’s
 cerebral cortex tissue sample \* TolProt value-CTX: numeric value of Total
 Protein obtained from the subject’s cerebral cortex tissue sample \*
 Normalized Sample CTX D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebral cortex tissue sample \* Blot Mean of CTX Normalized Euploid
 Samples: average of the normalized DYRK1A values from all euploid
 subjects’ cerebral cortex tissue samples on the blot \* Relative Ratio CTX
 (Norm Sample/Blot Mean of Norm Eu): relative ratio of the normalized
 DYRK1A value from the subject’s cerebral cortex tissue sample compared to
 the average normalized DYRK1A value of all euploid subjects’ cerebral
 cortex tissue samples on the blot \* Z-Score CTX: Standard scores of the
 subject’s cerebellar DYRK1A relative intensity, obtained by converting
 each relative intensity value to a standard score corresponding to the
 number of standard deviation units above or below the mean of the
 comparable euploid group \* % of Control CTX: relative ratio of DYRK1A in
 the subject’s cerebellar tissue sample converted to a percentage \* D1A
 value-CB: optical density value of DYRK1A obtained from the subject’s
 cerebellar tissue sample \* TolProt value-CB: numeric value of Total
 Protein obtained from the subject’s cerebellar tissue sample \* Normalized
 Sample CB D1A/TotProt: normalized value of DYRK1A optical density divided
 by the numeric value of total protein within the subject’s cerebellar
 tissue sample \* Blot Mean of CB Normalized Euploid Samples: average of
 the normalized DYRK1A values from all euploid subjects’ cerebellar tissue
 samples on the blot \* Relative Ratio CB (Norm Sample/Blot Mean of Norm
 Eu): relative ratio of the normalized DYRK1A value from the subject’s
 cerebellar tissue sample compared to the average normalized DYRK1A value
 of all euploid subjects’ cerebellar tissue samples on the blot \* Z-Score
 CB: Standard scores of the subject’s cerebellar DYRK1A relative intensity,
 obtained by converting each relative intensity value to a standard score
 corresponding to the number of standard deviation units above or below the
 mean of the comparable euploid group \* % of Control CB: relative ratio of
 DYRK1A in the subject’s cerebellar tissue sample converted to a percentage
 4\. Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_KnckDwn_P06.xlsx 1\.
 Number of variables: 23 2\. Number of cases/rows: 56 3\. Variable List: \*
 DT Animal number: unique identification number assigned to the subject \*
 Blot number: Western blot identification number on which the subject’s
 samples were analyzed \* Age: postnatal age of the subject at tissue
 collection \* F=1, M=2: sex (female = 1; male = 2) \* Genotype: genotype
 (Eu+/-: Euploid with 1 functional Dyrk1a allele; Eu+/+: Euploid with 2
 functional Dyrk1a alleles; Tri+/+/+: Trisomic with 3 functional Dyrk1a
 alleles; Tri+/+/-: Trisomic with 2 functional Dyrk1a alleles) \* GenoCode:
 numeric code corresponding to the subject’s genotype (1= Eu+/-; 2= Eu+/+;
 3= Tri+/+/+; 4= Tri+/+/-) \* Homog. Lanes: the number of wells/lanes on
 the Western blot that contained a homogenate protein sample used as a
 standard for comparison \* Homogenate Norm Mean: average of the normalized
 DYRK1A values from all homogenate protein samples on the blot standard \*
 D1A value-HIP: optical density value of DYRK1A obtained from the subject’s
 hippocampal tissue sample \* TolProt value-HIP: numeric value of Total
 Protein obtained from the subject’s hippocampal tissue sample \*
 Normalized Sample HIP D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 hippocampal tissue sample \* Blot Mean of HIP Normalized Euploid Samples:
 average of the normalized DYRK1A values from all euploid subjects’
 hippocampal tissue samples on the blot \* HIP Rel Intensity to Homogenate:
 relative ratio of the normalized DYRK1A value from the subject’s
 hippocampal tissue sample compared to the average normalized DYRK1A value
 of homogenate samples on the blot \* D1A value-CTX: optical density value
 of DYRK1A obtained from the subject’s cerebral cortex tissue sample \*
 TolProt value-CTX: numeric value of Total Protein obtained from the
 subject’s cerebral cortex tissue sample \* Normalized Sample CTX
 D1A/TotProt: normalized value of DYRK1A optical density divided by the
 numeric value of total protein within the subject’s cerebral cortex tissue
 sample \* CTX Rel Intensity to Homogenate: relative ratio of the
 normalized DYRK1A value from the subject’s cerebral cortex tissue sample
 compared to the average normalized DYRK1A value of homogenate samples on
 the blot \* D1A value-CB: optical density value of DYRK1A obtained from
 the subject’s cerebellar tissue sample \* TolProt value-CTX: numeric value
 of Total Protein obtained from the subject’s cerebellar tissue sample \*
 Normalized Sample CB D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebellar tissue sample \* CB Rel Intensity to Homogenate: relative ratio
 of the normalized DYRK1A value from the subject’s cerebellar cortex tissue
 sample compared to the average normalized DYRK1A value of homogenate
 samples on the blot \* HIP %EuControl: percentage of DYRK1A relative
 intensity in the subject’s hippocampal tissue sample compared the
 normalized euploid value of the same brain region on the same blot \* CTX
 %EuControl: percentage of DYRK1A relative intensity in the subject’s
 cerebral cortex tissue sample compared the normalized euploid value of the
 same brain region on the same blot \* CB %EuControl: percentage of DYRK1A
 relative intensity in the subject’s hippocampal tissue sample compared the
 normalized euploid value of the same brain region on the same blot 4\.
 Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_WBData_KnckDwn_P15.xlsx 1\.
 Number of variables: 23 2\. Number of cases/rows: 48 3\. Variable List: \*
 DT Animal number: unique identification number assigned to the subject \*
 Blot number: Western blot identification number on which the subject’s
 samples were analyzed \* Age: postnatal age of the subject at tissue
 collection \* F=1, M=2: sex (female = 1; male = 2) \* Genotype: genotype
 (Eu+/-: Euploid with 1 functional Dyrk1a allele; Eu+/+: Euploid with 2
 functional Dyrk1a alleles; Tri+/+/+: Trisomic with 3 functional Dyrk1a
 alleles; Tri+/+/-: Trisomic with 2 functional Dyrk1a alleles) \* GenoCode:
 numeric code of the subject’s genotype (1= Eu+/-; 2= Eu+/+; 3= Tri+/+/+;
 4= Tri+/+/-) \* # Homog.Lanes: the number of wells/lanes on the Western
 blot that contained a homogenate protein sample used as a standard for
 comparison \* Homogenate Norm Mean: average of the normalized DYRK1A
 values from all homogenate protein samples on the blot standard \* D1A
 value-HIP: optical density value of DYRK1A obtained from the subject’s
 hippocampal tissue sample \* TolProt value-HIP: numeric value of Total
 Protein obtained from the subject’s hippocampal tissue sample \*
 Normalized Sample HIP D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 hippocampal tissue sample \* Blot Mean of HIP Normalized Euploid Samples:
 average of the normalized DYRK1A values from all euploid subjects’
 hippocampal tissue samples on the blot \* HIP Rel Intensity to Homogenate:
 relative ratio of the normalized DYRK1A value from the subject’s
 hippocampal tissue sample compared to the average normalized DYRK1A value
 of homogenate samples on the blot \* D1A value-CTX: optical density value
 of DYRK1A obtained from the subject’s cerebral cortex tissue sample \*
 TolProt value-CTX: numeric value of Total Protein obtained from the
 subject’s cerebral cortex tissue sample \* Normalized Sample CTX
 D1A/TotProt: normalized value of DYRK1A optical density divided by the
 numeric value of total protein within the subject’s cerebral cortex tissue
 sample \* CTX Rel Intensity to Homogenate: relative ratio of the
 normalized DYRK1A value from the subject’s cerebral cortex tissue sample
 compared to the average normalized DYRK1A value of homogenate samples on
 the blot \* D1A value-CB: optical density value of DYRK1A obtained from
 the subject’s cerebellar tissue sample \* TolProt value-CTX: numeric value
 of Total Protein obtained from the subject’s cerebellar tissue sample \*
 Normalized Sample CB D1A/TotProt: normalized value of DYRK1A optical
 density divided by the numeric value of total protein within the subject’s
 cerebellar tissue sample \* CB Rel Intensity to Homogenate: relative ratio
 of the normalized DYRK1A value from the subject’s cerebellar cortex tissue
 sample compared to the average normalized DYRK1A value of homogenate
 samples on the blot \* HIP %EuControl: percentage of DYRK1A relative
 intensity in the subject’s hippocampal tissue sample compared the
 normalized euploid value of the same brain region on the same blot \* CTX
 %EuControl: percentage of DYRK1A relative intensity in the subject’s
 cerebral cortex tissue sample compared the normalized euploid value of the
 same brain region on the same blot \* CB %EuControl: percentage of DYRK1A
 relative intensity in the subject’s hippocampal tissue sample compared the
 normalized euploid value of the same brain region on the same blot 4\.
 Missing data codes: NA (data not available); "." or
 "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_RTqPCR_P0.xlsx 1\. Number of
 variables: 42 2\. Number of cases/rows: 38 3\. Variable List: \* Plate#:
 identification number of the qPCR plate on which the sample was analyzed
 \* Animal#: unique identification number assigned to the subject \* Age:
 postnatal age of the subject at tissue collection \* SexCode: Sex (1=
 female; 2= male) \* GenoCode: numeric code corresponding to the subject’s
 genotype (1 = euploid/control; 2 = Ts65Dn/trisomic) \* Rn CT FB1:
 forebrain Rn18s cycle threshold, replicate 1 \* Rn CT FB2: forebrain Rn18s
 cycle threshold, replicate 2 \* Rn CT FB3: forebrain Rn18s cycle
 threshold, replicate 3 \* Rn18s CT FB GEOMEAN: geometric mean the 3
 replicated Rn18s cycle threshold values \* Dyrk5-6 CT FB1: forebrain
 Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1 \* Dyrk5-6
 CT FB2: forebrain Dyrk1a cycle threshold, probe targeting exons 5-6,
 replicate 2 \* Dyrk5-6 CT FB3: forebrain Dyrk1a cycle threshold, probe
 targeting exons 5-6, replicate 3 \* Dyrk5-6 CT FB AVG: average of the
 subject’s 3 replicated Dyrk1a5-6 cycle threshold values \* ?CT Dyrk5-6 FB:
 difference in cycle threshold values ((average Dyrk1a5-6 cycle threshold
 value) – (average Rn18s cycle threshold value)) \* FB Dyrk5-6 2-(?CT):
 2-(?CT) value calculated from the ?CT value \* FB Dyrk5-6 Fold Change
 within plate: subject’s forebrain Dyrk5-6 2-(?CT) value divided by the
 matched mean euploid 2-(?CT) value of the comparable group from the same
 qPCR plate \* Dyrk10-11 CT FB1: forebrain Dyrk1a cycle threshold, probe
 targeting exons 10-11, replicate 1 \* Dyrk10-11 CT FB2: forebrain Dyrk1a
 cycle threshold, probe targeting exons 10-11, replicate 2 \* Dyrk10-11 CT
 FB3: forebrain Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 3 \* Dyrk10-11 CT FB AVG: average of the subject’s 3 replicated
 Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11 FB: difference in
 cycle threshold values ((average Dyrk1a10-11 cycle threshold value) –
 (average Rn18s cycle threshold value)) \* FB Dyrk10-11 Fold Change within
 plate: subject’s forebrain Dyrk10-11 2-(?CT) value divided by the mean
 euploid 2-(?CT) value of the comparable group from the same qPCR plate \*
 Rn CT CB1: cerebellar Rn18s cycle threshold, replicate 1 \* Rn CT CB2:
 cerebellar Rn18s cycle threshold, replicate 2 \* Rn CT CB3: cerebellar
 Rn18s cycle threshold, replicate 3 \* Rn18s CT CB GEOMEAN: geometric mean
 the 3 replicated Rn18s cycle threshold values \* Dyrk5-6 CT CB1:
 cerebellar Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1
 \* Dyrk5-6 CT CB2: cerebellar Dyrk1a cycle threshold, probe targeting
 exons 5-6, replicate 2 \* Dyrk5-6 CT CB3: cerebellar Dyrk1a cycle
 threshold, probe targeting exons 5-6, replicate 3 \* Dyrk5-6 CT CB AVG:
 average of the subject’s 3 replicated cerebellar Dyrk1a5-6 cycle threshold
 values \* ?CT Dyrk5-6 CB: difference in cycle threshold values ((average
 Dyrk1a5-6 cycle threshold value) – (average Rn18s cycle threshold value))
 \* CB Dyrk5-6 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CB
 Dyrk5-6 Fold Change within plate: subject’s cerebellar Dyrk5-6 2-(?CT)
 value divided by the matched mean euploid 2-(?CT) value of the comparable
 group from the same qPCR plate \* Dyrk10-11 CT CB1: cerebellar Dyrk1a
 cycle threshold, probe targeting exons 10-11, replicate 1 \* Dyrk10-11 CT
 CB2: cerebellar Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 2 \* Dyrk10-11 CT CB3: cerebellar Dyrk1a cycle threshold, probe
 targeting exons 10-11, replicate 3 \* Dyrk10-11 CT CB AVG: average of the
 subject’s 3 replicated Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11
 CB: difference in cycle threshold values ((average Dyrk1a10-11 cycle
 threshold value) – (average Rn18s cycle threshold value)) \* CB Dyrk10-11
 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CB Dyrk10-11 Fold
 Change within plate: subject’s cerebellar Dyrk10-11 2-(?CT) value divided
 by the mean euploid 2-(?CT) value of the comparable group from the same
 qPCR plate 4\. Missing data codes: NA (data not available); "."
 or "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_RTqPCR_P3.xlsx 1\. Number of
 variables: 41 2\. Number of cases/rows: 32 3\. Variable List: \* Plate#:
 identification number of the qPCR plate on which the sample was analyzed
 \* Animal#: unique identification number assigned to the subject \* Age:
 postnatal age of the subject at tissue collection \* SexCode: Sex (1=
 female; 2= male) \* GenoCode: numeric code corresponding to the subject’s
 genotype (1 = euploid/control; 2 = Ts65Dn/trisomic) \* Rn CT FB1:
 forebrain Rn18s cycle threshold, replicate 1 \* Rn CT FB2: forebrain Rn18s
 cycle threshold, replicate 2 \* Rn CT FB3: forebrain Rn18s cycle
 threshold, replicate 3 \* Rn18s CT FB GEOMEAN: geometric mean the 3
 replicated Rn18s cycle threshold values \* Dyrk5-6 CT FB1: forebrain
 Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1 \* Dyrk5-6
 CT FB2: forebrain Dyrk1a cycle threshold, probe targeting exons 5-6,
 replicate 2 \* Dyrk5-6 CT FB3: forebrain Dyrk1a cycle threshold, probe
 targeting exons 5-6, replicate 3 \* Dyrk5-6 CT FB AVG: average of the
 subject’s 3 replicated Dyrk1a5-6 cycle threshold values \* ?CT Dyrk5-6 FB:
 difference in cycle threshold values ((average Dyrk1a5-6 cycle threshold
 value) – (average Rn18s cycle threshold value)) \* FB Dyrk5-6 2-(?CT):
 2-(?CT) value calculated from the ?CT value \* FB Dyrk5-6 Fold Change
 within plate: subject’s forebrain Dyrk5-6 2-(?CT) value divided by the
 matched mean euploid 2-(?CT) value of the comparable group from the same
 qPCR plate \* Dyrk10-11 CT FB1: forebrain Dyrk1a cycle threshold, probe
 targeting exons 10-11, replicate 1 \* Dyrk10-11 CT FB2: forebrain Dyrk1a
 cycle threshold, probe targeting exons 10-11, replicate 2 \* Dyrk10-11 CT
 FB3: forebrain Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 3 \* Dyrk10-11 CT FB AVG: average of the subject’s 3 replicated
 Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11 FB: difference in
 cycle threshold values ((average Dyrk1a10-11 cycle threshold value) –
 (average Rn18s cycle threshold value)) \* FB Dyrk10-11 2-(?CT): 2-(?CT)
 value calculated from the ?CT value \* FB Dyrk10-11 Fold Change within
 plate: subject’s forebrain Dyrk10-11 2-(?CT) value divided by the mean
 euploid 2-(?CT) value of the comparable group from the same qPCR plate \*
 Rn CT CB1: cerebellar Rn18s cycle threshold, replicate 1 \* Rn CT CB2:
 cerebellar Rn18s cycle threshold, replicate 2 \* Rn CT CB3: cerebellar
 Rn18s cycle threshold, replicate 3 \* Rn18s CT CB GEOMEAN: geometric mean
 the 3 replicated Rn18s cycle threshold values \* Dyrk5-6 CT CB1:
 cerebellar Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1
 \* Dyrk5-6 CT CB2: cerebellar Dyrk1a cycle threshold, probe targeting
 exons 5-6, replicate 2 \* Dyrk5-6 CT CB3: cerebellar Dyrk1a cycle
 threshold, probe targeting exons 5-6, replicate 3 \* Dyrk5-6 CT CB AVG:
 average of the subject’s 3 replicated cerebellar Dyrk1a5-6 cycle threshold
 values \* ?CT Dyrk5-6 CB: difference in cycle threshold values ((average
 Dyrk1a5-6 cycle threshold value) – (average Rn18s cycle threshold value))
 \* CB Dyrk5-6 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CB
 Dyrk5-6 Fold Change within plate: subject’s cerebellar Dyrk5-6 2-(?CT)
 value divided by the matched mean euploid 2-(?CT) value of the comparable
 group from the same qPCR plate \* Dyrk10-11 CT CB1: cerebellar Dyrk1a
 cycle threshold, probe targeting exons 10-11, replicate 1 \* Dyrk10-11 CT
 CB2: cerebellar Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 2 \* Dyrk10-11 CT CB3: cerebellar Dyrk1a cycle threshold, probe
 targeting exons 10-11, replicate 3 \* Dyrk10-11 CT CB AVG: average of the
 subject’s 3 replicated Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11
 CB: difference in cycle threshold values ((average Dyrk1a10-11 cycle
 threshold value) – (average Rn18s cycle threshold value)) \* CB Dyrk10-11
 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CB Dyrk10-11 Fold
 Change within plate: subject’s cerebellar Dyrk10-11 2-(?CT) value divided
 by the mean euploid 2-(?CT) value of the comparable group from the same
 qPCR plate 4\. Missing data codes: NA (data not available); "."
 or "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_RTqPCR_P6.xlsx 1\. Number of
 variables: 59 2\. Number of cases/rows: 24 3\. Variable List: \* Plate#:
 identification number of the qPCR plate on which the sample was analyzed
 \* Animal#: unique identification number assigned to the subject \* Age:
 postnatal age of the subject at tissue collection \* SexCode: Sex (1=
 female; 2= male) \* GenoCode: numeric code corresponding to the subject’s
 genotype (1 = euploid/control; 2 = Ts65Dn/trisomic) \* Rn CT HIPP1:
 hippocampus Rn18s cycle threshold, replicate 1 \* Rn CT HIPP: hippocampus
 Rn18s cycle threshold, replicate 2 \* Rn CT HIPP3: hippocampus Rn18s cycle
 threshold, replicate 3 \* Rn18s CT HIPP GEOMEAN: geometric mean the 3
 replicated Rn18s cycle threshold values \* Dyrk5-6 CT HIPP1: hippocampus
 Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1 \* Dyrk5-6
 CT HIPP2: hippocampus Dyrk1a cycle threshold, probe targeting exons 5-6,
 replicate 2 \* Dyrk5-6 CT HIPP3: hippocampus Dyrk1a cycle threshold, probe
 targeting exons 5-6, replicate 3 \* Dyrk5-6 CT HIPP AVG: average of the
 subject’s 3 replicated Dyrk1a5-6 cycle threshold values \* ?CT Dyrk5-6
 HIPP: difference in cycle threshold values ((average Dyrk1a5-6 cycle
 threshold value) – (average Rn18s cycle threshold value)) \* HIPP Dyrk5-6
 2-(?CT): 2-(?CT) value calculated from the ?CT value \* HIPP Dyrk5-6 Fold
 Change within plate: subject’s hippocampus Dyrk5-6 2-(?CT) value divided
 by the matched mean euploid 2-(?CT) value of the comparable group from the
 same qPCR plate \* Dyrk10-11 CT HIPP1: hippocampus Dyrk1a cycle threshold,
 probe targeting exons 10-11, replicate 1 \* Dyrk10-11 CT HIPP: hippocampus
 Dyrk1a cycle threshold, probe targeting exons 10-11, replicate 2 \*
 Dyrk10-11 CT HIPP3: hippocampus Dyrk1a cycle threshold, probe targeting
 exons 10-11, replicate 3 \* Dyrk10-11 CT HIPP AVG: average of the
 subject’s 3 replicated Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11
 HIPP: difference in cycle threshold values ((average Dyrk1a10-11 cycle
 threshold value) – (average Rn18s cycle threshold value)) \* HIPP
 Dyrk10-11 2-(?CT): 2-(?CT) value calculated from the ?CT value \* HIPP
 Dyrk10-11 Fold Change within plate: subject’s hippocampus Dyrk10-11
 2-(?CT) value divided by the mean euploid 2-(?CT) value of the comparable
 group from the same qPCR plate \* Rn CT CTX1: cerebral cortex Rn18s cycle
 threshold, replicate 1 \* Rn CT CTX2: cerebral cortex Rn18s cycle
 threshold, replicate 2 \* Rn CT CTX3: cerebral cortex Rn18s cycle
 threshold, replicate 3 \* Rn18s CT CTX GEOMEAN: geometric mean the 3
 replicated Rn18s cycle threshold values \* Dyrk5-6 CT CTX1: cerebral
 cortex Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1 \*
 Dyrk5-6 CT CTX2: cerebral cortex Dyrk1a cycle threshold, probe targeting
 exons 5-6, replicate 2 \* Dyrk5-6 CT CTX3: cerebral cortex Dyrk1a cycle
 threshold, probe targeting exons 5-6, replicate 3 \* Dyrk5-6 CT CTX AVG:
 average of the subject’s 3 replicated cerebral cortex Dyrk1a5-6 cycle
 threshold values \* ?CT Dyrk5-6 CTX: difference in cycle threshold values
 ((average Dyrk1a5-6 cycle threshold value) – (average Rn18s cycle
 threshold value)) \* CTX Dyrk5-6 2-(?CT): 2-(?CT) value calculated from
 the ?CT value \* CTX Dyrk5-6 Fold Change within plate: subject’s cerebral
 cortex Dyrk5-6 2-(?CT) value divided by the matched mean euploid 2-(?CT)
 value of the comparable group from the same qPCR plate \* Dyrk10-11 CT
 CTX1: cerebral cortex Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 1 \* Dyrk10-11 CT CTX2: cerebral cortex Dyrk1a cycle threshold,
 probe targeting exons 10-11, replicate 2 \* Dyrk10-11 CT CTX3: cerebral
 cortex Dyrk1a cycle threshold, probe targeting exons 10-11, replicate 3 \*
 Dyrk10-11 CT CTX AVG: average of the subject’s 3 replicated Dyrk1a10-11
 cycle threshold values \* ?CT Dyrk10-11 CTX: difference in cycle threshold
 values ((average Dyrk1a10-11 cycle threshold value) – (average Rn18s cycle
 threshold value)) \* CTX Dyrk10-11 2-(?CT): 2-(?CT) value calculated from
 the ?CT value \* CTX Dyrk10-11 Fold Change within plate: subject’s
 cerebral cortex Dyrk10-11 2-(?CT) value divided by the mean euploid
 2-(?CT) value of the comparable group from the same qPCR plate \* Rn CT
 CB1: cerebellar Rn18s cycle threshold, replicate 1 \* Rn CT CB2:
 cerebellar Rn18s cycle threshold, replicate 2 \* Rn CT CB3: cerebellar
 Rn18s cycle threshold, replicate 3 \* Rn18s CT CB GEOMEAN: geometric mean
 the 3 replicated Rn18s cycle threshold values \* Dyrk5-6 CT CB1:
 cerebellar Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 1
 \* Dyrk5-6 CT CB2: cerebellar Dyrk1a cycle threshold, probe targeting
 exons 5-6, replicate 2 \* Dyrk5-6 CT CB3: cerebellar Dyrk1a cycle
 threshold, probe targeting exons 5-6, replicate 3 \* Dyrk5-6 CT CB AVG:
 average of the subject’s 3 replicated cerebellar Dyrk1a5-6 cycle threshold
 values \* ?CT Dyrk5-6 CB: difference in cycle threshold values ((average
 Dyrk1a5-6 cycle threshold value) – (average Rn18s cycle threshold value))
 \* CB Dyrk5-6 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CB
 Dyrk5-6 Fold Change within plate: subject’s cerebellar Dyrk5-6 2-(?CT)
 value divided by the matched mean euploid 2-(?CT) value of the comparable
 group from the same qPCR plate \* Dyrk10-11 CT CB1: cerebellar Dyrk1a
 cycle threshold, probe targeting exons 10-11, replicate 1 \* Dyrk10-11 CT
 CB2: cerebellar Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 2 \* Dyrk10-11 CT CB3: cerebellar Dyrk1a cycle threshold, probe
 targeting exons 10-11, replicate 3 \* Dyrk10-11 CT CB AVG: average of the
 subject’s 3 replicated Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11
 CB: difference in cycle threshold values ((average Dyrk1a10-11 cycle
 threshold value) – (average Rn18s cycle threshold value)) \* CB Dyrk10-11
 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CB Dyrk10-11 Fold
 Change within plate: subject’s cerebellar Dyrk10-11 2-(?CT) value divided
 by the mean euploid 2-(?CT) value of the comparable group from the same
 qPCR plate 4\. Missing data codes: NA (data not available); "."
 or "x" indicate missing data for an individual 5\. Specialized
 formats or other abbreviations used: None
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Hawley_2023_RTqPCR_KnckDwn_P06.xlsx 1\.
 Number of variables: 59 2\. Number of cases/rows: 19 3\. Variable List: \*
 Plate#: identification number of the qPCR plate on which the sample was
 analyzed \* Animal#: unique identification number assigned to the subject
 \* Age: postnatal age of the subject at tissue collection \* SexCode: Sex
 (1= female; 2= male) \* GenoCode: numeric code corresponding to the
 subjet's genotype (2 = euploid with 2 functional Dyrk1a alleles; 3 =
 trisomic with 3 functional Dyrk1a alleles; 4 = trisomic with 2 functional
 Dyrk1a alleles) \* Rn CT HIPP1: hippocampus Rn18s cycle threshold,
 replicate 1 \* Rn CT HIPP: hippocampus Rn18s cycle threshold, replicate 2
 \* Rn CT HIPP3: hippocampus Rn18s cycle threshold, replicate 3 \* Rn18s CT
 HIPP GEOMEAN: geometric mean the 3 replicated Rn18s cycle threshold values
 \* Dyrk5-6 CT HIPP1: hippocampus Dyrk1a cycle threshold, probe targeting
 exons 5-6, replicate 1 \* Dyrk5-6 CT HIPP2: hippocampus Dyrk1a cycle
 threshold, probe targeting exons 5-6, replicate 2 \* Dyrk5-6 CT HIPP3:
 hippocampus Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 3
 \* Dyrk5-6 CT HIPP AVG: average of the subject’s 3 replicated Dyrk1a5-6
 cycle threshold values \* ?CT Dyrk5-6 HIPP: difference in cycle threshold
 values ((average Dyrk1a5-6 cycle threshold value) – (average Rn18s cycle
 threshold value)) \* HIPP Dyrk5-6 2-(?CT): 2-(?CT) value calculated from
 the ?CT value \* HIPP Dyrk5-6 Fold Change within plate: subject’s
 hippocampus Dyrk5-6 2-(?CT) value divided by the matched mean euploid
 2-(?CT) value of the comparable group from the same qPCR plate \*
 Dyrk10-11 CT HIPP1: hippocampus Dyrk1a cycle threshold, probe targeting
 exons 10-11, replicate 1 \* Dyrk10-11 CT HIPP: hippocampus Dyrk1a cycle
 threshold, probe targeting exons 10-11, replicate 2 \* Dyrk10-11 CT HIPP3:
 hippocampus Dyrk1a cycle threshold, probe targeting exons 10-11, replicate
 3 \* Dyrk10-11 CT HIPP AVG: average of the subject’s 3 replicated
 Dyrk1a10-11 cycle threshold values \* ?CT Dyrk10-11 HIPP: difference in
 cycle threshold values ((average Dyrk1a10-11 cycle threshold value) –
 (average Rn18s cycle threshold value)) \* HIPP Dyrk10-11 2-(?CT): 2-(?CT)
 value calculated from the ?CT value \* HIPP Dyrk10-11 Fold Change within
 plate: subject’s hippocampus Dyrk10-11 2-(?CT) value divided by the mean
 euploid 2-(?CT) value of the comparable group from the same qPCR plate \*
 Rn CT CTX1: cerebral cortex Rn18s cycle threshold, replicate 1 \* Rn CT
 CTX2: cerebral cortex Rn18s cycle threshold, replicate 2 \* Rn CT CTX3:
 cerebral cortex Rn18s cycle threshold, replicate 3 \* Rn18s CT CTX
 GEOMEAN: geometric mean the 3 replicated Rn18s cycle threshold values \*
 Dyrk5-6 CT CTX1: cerebral cortex Dyrk1a cycle threshold, probe targeting
 exons 5-6, replicate 1 \* Dyrk5-6 CT CTX2: cerebral cortex Dyrk1a cycle
 threshold, probe targeting exons 5-6, replicate 2 \* Dyrk5-6 CT CTX3:
 cerebral cortex Dyrk1a cycle threshold, probe targeting exons 5-6,
 replicate 3 \* Dyrk5-6 CT CTX AVG: average of the subject’s 3 replicated
 cerebral cortex Dyrk1a5-6 cycle threshold values \* ?CT Dyrk5-6 CTX:
 difference in cycle threshold values ((average Dyrk1a5-6 cycle threshold
 value) – (average Rn18s cycle threshold value)) \* CTX Dyrk5-6 2-(?CT):
 2-(?CT) value calculated from the ?CT value \* CTX Dyrk5-6 Fold Change
 within plate: subject’s cerebral cortex Dyrk5-6 2-(?CT) value divided by
 the matched mean euploid 2-(?CT) value of the comparable group from the
 same qPCR plate \* Dyrk10-11 CT CTX1: cerebral cortex Dyrk1a cycle
 threshold, probe targeting exons 10-11, replicate 1 \* Dyrk10-11 CT CTX2:
 cerebral cortex Dyrk1a cycle threshold, probe targeting exons 10-11,
 replicate 2 \* Dyrk10-11 CT CTX3: cerebral cortex Dyrk1a cycle threshold,
 probe targeting exons 10-11, replicate 3 \* Dyrk10-11 CT CTX AVG: average
 of the subject’s 3 replicated Dyrk1a10-11 cycle threshold values \* ?CT
 Dyrk10-11 CTX: difference in cycle threshold values ((average Dyrk1a10-11
 cycle threshold value) – (average Rn18s cycle threshold value)) \* CTX
 Dyrk10-11 2-(?CT): 2-(?CT) value calculated from the ?CT value \* CTX
 Dyrk10-11 Fold Change within plate: subject’s cerebral cortex Dyrk10-11
 2-(?CT) value divided by the mean euploid 2-(?CT) value of the comparable
 group from the same qPCR plate \* Rn CT CB1: cerebellar Rn18s cycle
 threshold, replicate 1 \* Rn CT CB2: cerebellar Rn18s cycle threshold,
 replicate 2 \* Rn CT CB3: cerebellar Rn18s cycle threshold, replicate 3 \*
 Rn18s CT CB GEOMEAN: geometric mean the 3 replicated Rn18s cycle threshold
 values \* Dyrk5-6 CT CB1: cerebellar Dyrk1a cycle threshold, probe
 targeting exons 5-6, replicate 1 \* Dyrk5-6 CT CB2: cerebellar Dyrk1a
 cycle threshold, probe targeting exons 5-6, replicate 2 \* Dyrk5-6 CT CB3:
 cerebellar Dyrk1a cycle threshold, probe targeting exons 5-6, replicate 3
 \* Dyrk5-6 CT CB AVG: average of the subject’s 3 replicated cerebellar
 Dyrk1a5-6 cycle threshold values \* ?CT Dyrk5-6 CB: difference in cycle
 threshold values ((average Dyrk1a5-6 cycle threshold value) – (average
 Rn18s cycle threshold value)) \* CB Dyrk5-6 2-(?CT): 2-(?CT) value
 calculated from the ?CT value \* CB Dyrk5-6 Fold Change within plate:
 subject’s cerebellar Dyrk5-6 2-(?CT) value divided by the matched mean
 euploid 2-(?CT) value of the comparable group from the same qPCR plate \*
 Dyrk10-11 CT CB1: cerebellar Dyrk1a cycle threshold, probe targeting exons
 10-11, replicate 1 \* Dyrk10-11 CT CB2: cerebellar Dyrk1a cycle threshold,
 probe targeting exons 10-11, replicate 2 \* Dyrk10-11 CT CB3: cerebellar
 Dyrk1a cycle threshold, probe targeting exons 10-11, replicate 3 \*
 Dyrk10-11 CT CB AVG: average of the subject’s 3 replicated Dyrk1a10-11
 cycle threshold values \* ?CT Dyrk10-11 CB: difference in cycle threshold
 values ((average Dyrk1a10-11 cycle threshold value) – (average Rn18s cycle
 threshold value)) \* CB Dyrk10-11 2-(?CT): 2-(?CT) value calculated from
 the ?CT value \* CB Dyrk10-11 Fold Change within plate: subject’s
 cerebellar Dyrk10-11 2-(?CT) value divided by the mean euploid 2-(?CT)
 value of the comparable group from the same qPCR plate 4\. Missing data
 codes: NA (data not available); "." or "x" indicate
 missing data for an individual 5\. Specialized formats or other
 abbreviations used: None