cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/06t9j-ats62
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C111
description: <strong>Tilt Series Date:</strong> 2012-02-18</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-6/3dimage_68737/</p>12feb18_C111_pNL_INF_AT1BT10006_part122_11.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-6/3dimage_68737/12feb18_C111_pNL_INF_AT1BT10006_part122_11.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb18_C111_pNL_INF_AT1BT10006.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-6/rawdata/12feb18_C111_pNL_INF_AT1BT10006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ebke6k
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Atoconeura kenya, Atoconeura, Atoconeura eudoxia, Atoconeura aethiopica, Atoconeura luxata, Atoconeura biordinata, Allocnemis cyanura, Allocnemis, Trithemis, Elattoneura villiersi, Elattoneura, Thermochoria equivocata, Thermochoria, Thermochoria jeanneli, Ischnura elegans, Ischnura, Selysiothemis nigra, Selysiothemis, Hemistigma affine, Hemistigma, Aciagrion, Sympetrum fonscolombii, Sympetrum, Micromacromia camerunica, Micromacromia, Micromacromia zygoptera, Aethiothemis erythromelas, Aethiothemis, Aciagrion gracile, Aciagrion dondoense, Trithetrum navasi, Trithetrum, Trithetrum congoense, Aciagrion africanum, Trithemis tropicana, Hadrothemis vrijdaghi, Hadrothemis, Hadrothemis scabrifrons, Hadrothemis defecta, Hadrothemis camarensis, Hadrothemis versuta, Hadrothemis infesta, Hadrothemis coacta, Hemicordulia, Diastatomma multilineatum, Diastatomma, Mastigogomphus chapini, Mastigogomphus, Crenigomphus kavangoensis, Crenigomphus, Pseudagrion merina, Pseudagrion, Allocnemis marshalli, Hemicordulia asiatica, Microgomphus, Cordulegaster boltonii algirica, Cordulegaster boltonii, Cordulegaster, Hemicordulia similis, Allocnemis elongata, Orthetrum malgassicum, Orthetrum, Lestinogomphus silkeae, Lestinogomphus, Hemicordulia virens, Microgomphus nyassicus, Paragomphus serrulatus, Paragomphus, Aeshna isoceles, Aeshna, Neurocordulia, Mortonagrion stygium, Mortonagrion, Pyrrhosoma nymphula, Pyrrhosoma, Proplatycnemis pseudalatipes, Proplatycnemis, Neophya rutherfordi, Neophya, Allocnemis superba, Elattoneura vittata, Onychogomphus nigrotibialis, Onychogomphus, Cordulia, Cordulia aenea, Stenocypha molindica, Stenocypha, Platycypha picta, Platycypha, Syncordulia venator, Syncordulia, Syncordulia gracilis, Crocothemis, Metacnemis valida, Metacnemis, Crocothemis sanguinolenta, Crocothemis erythraea, Platycypha rufitibia, Crocothemis saxicolor, Crocothemis striata)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((45.7252 -9.5538,46.1382 -10.1057,46.1382 -8.8989,45.8555 -9.026,45.7252 -9.5538))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045943-250811113504898/datasets/export for details.
DOI: 10.22002/sfj9h-pcx58
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-04-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-23/3dimage_68619/</p>12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0006_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-28-23/3dimage_68619/12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0006_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr28_C126_2_pNLDN_COS7_AT3BT3trial_0006.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.6tkz3x
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hibiscus sabiensis"
 ]
}

 The dataset includes 13 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045944-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/nwyze-gbj30
title: HIV capsid purified trim5 alpha

description: 26/A2 slit B grid, 22500 8 defocus  0.3 exposure
description: <strong>Tilt Series Date:</strong> 2013-12-12</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 26.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod and SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified recombinant trim5 alpha decorated HIV cores</p>
<strong>Sample Preparation:</strong> B grid</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-12-4/rawdata/</p>Trim5alpha22500bgrid0004.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-12-4/rawdata/Trim5alpha22500bgrid0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        Trim5alpha22500bgrid0004_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-12-4/3dimage_35997/Trim5alpha22500bgrid0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8za1r-c5n24
title: UU 2618 tsr-QEQE

description: "wild type" tsr QEQE in R-B- background
description: <strong>Tilt Series Date:</strong> 2012-10-03</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB to log phase, lysed with penicillin for 1 hour</p>
<strong>Sample Preparation:</strong> plunge frozen on Quantifoil R12/2 grids in ethane/propane mix</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-03-6/rawdata/</p>strain1october0008.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-03-6/rawdata/strain1october0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        strain1october0008_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-03-6/3dimage_21008/strain1october0008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.njnuap
title: Occurrence Download

description: A dataset containing 23 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hibiscus saxatilis"
 ]
}

 The dataset includes 23 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045945-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/s0vvy-swx50
title: Alongum

description: Alongum_periplasmiclayers
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-50/rawdata/</p>alongLys_20031.mrc,  Tilt Series (Pixel Size 0.95 nm), 512.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-50/rawdata/alongLys_20031.mrc"
        > <i class="download icon"></i> Download </a></p>

        alongLys_20032_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-50/3dimage_56820/alongLys_20032_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hvk0s-t4577
title: Tprimitia_protK

description: Alongum_lysozyme
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-174/rawdata/</p>TrepProtK10013.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-174/rawdata/TrepProtK10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        TrepProtK10013_full.rec,  Reconstruction (Pixel Size 1.95 nm), 412.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-174/3dimage_57065/TrepProtK10013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.v22bqe
title: Occurrence Download

description: A dataset containing 157 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hibiscus shirensis"
 ]
}

 The dataset includes 157 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045946-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/44gey-e2c63
title: Bsubtilis_ponA_sacculi

description: Bsubtilis_sacculi_ponA
description: <strong>Tilt Series Date:</strong> 2012-02-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-7/rawdata/</p>MJsacculi0012.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-7/rawdata/MJsacculi0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        MJsacculi0012_part82_1.rec,  Reconstruction (Pixel Size 1.9 nm), 877.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-7/3dimage_26210/MJsacculi0012_part82_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w286w-pkk32
title: HUVECs infx pNLGFP

description: pNLGFP obtained from Irvin Chen (UCLA)
description: <strong>Tilt Series Date:</strong> 2013-10-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 36h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-22-11/rawdata/</p>C169-2_HUVECs_pNL36h_atlas3_0010.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-22-11/rawdata/C169-2_HUVECs_pNL36h_atlas3_0010.mrc"
        > <i class="download icon"></i> Download </a></p>

        C169-2_HUVECs_pNL36h_atlas3_0010_full.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8j7xw-09882
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2012-11-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-47/rawdata/</p>Bdellovibrio_wt_20121114_0030006.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-47/rawdata/Bdellovibrio_wt_20121114_0030006.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdellovibrio_wt_20121114_0030006_part122_4.rec,  Reconstruction (Pixel Size 1.32 nm), 441.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zq8ms-85434
title: UU 2801- P221D delta P1P2

description: Strain P221D (low kinase activity) with P1P2 deletion in CheA
description: <strong>Tilt Series Date:</strong> 2012-09-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase, then lysed for one hour with Penicillin</p>
<strong>Sample Preparation:</strong> cells plunge frozen on Quantifoul R2/2 in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-15/rawdata/</p>Coli2801atlas0917_10011.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-15/rawdata/Coli2801atlas0917_10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        Coli2801atlas0917_10011_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-15/3dimage_20836/Coli2801atlas0917_10011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2f911-vfa06
title: E. Coli delta53

description: Mutant MreB with sfGFP in sandwich position. WT MreB is replaced at the original locus with this fusion.
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia Coli </p>
<strong>Strain:</strong> delta53</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Josh Schaevitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 celsius to OD 600 ~ 0.3</p>
<strong>Sample Preparation:</strong> Blotted manually from rear in vitrobot at 100% humidity</p>
  mst2014-04-01-37_slicer9645.jpg: Escherichia coli .  filament parallel to membrane
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-01-37/rawdata/</p>040114_delta53_0008.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        040114_delta53_0008_full.rec,  Reconstruction (Pixel Size 1.68 nm), 861.2 MB  
        
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