cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7899794
title: Decreased platelet responsiveness to clopidogrel correlates with CYP2C19 and PON1 polymorphisms in atherosclerotic patients

description: Clopidogrel and aspirin are the most commonly used medications worldwide for dual antiplatelet therapy after percutaneous coronary intervention. However, clopidogrel hyporesponsiveness related to gene polymorphisms is a concern. Populations with higher degrees of genetic admixture may have increased prevalence of clopidogrel hyporesponsiveness. To assess this, we genotyped CYP2C19, ABCB1, and PON1 in 187 patients who underwent percutaneous coronary intervention. Race was self-defined by patients. We also performed light transmission aggregometry with adenosine diphosphate (ADP) and arachidonic acid during dual antiplatelet therapy. We found a significant difference for presence of the CYP2C19*2 polymorphism between white and non-white patients. Although 7% of patients had platelet resistance to clopidogrel, this did not correlate with any of the tested genetic polymorphisms. We did not find platelet resistance to aspirin in this cohort. Multivariate analysis showed that patients with PON1 and CYP2C19 polymorphisms had higher light transmission after ADP aggregometry than patients with native alleles. There was no preponderance of any race in patients with higher light transmission aggregometry. In brief, PON1 and CYP2C19 polymorphisms were associated with lower clopidogrel responsiveness in this sample. Despite differences in CYP2C19 polymorphisms across white and non-white patients, genetic admixture by itself was not able to identify clopidogrel hyporesponsiveness.
DOI: 10.6084/m9.figshare.7899797
title: Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements

description: IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven B-ALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of B-ALL, introduced for the first time in Tunisian laboratories.
DOI: 10.6084/m9.figshare.7899800
title: Dumbbell Shaped Microstrip Broadband Antenna

description: Abstract In this article, a dumbbell shaped microstrip broadband microstrip antenna with partial ground plane is presented, The overall dimension of the proposed antenna is 20 × 15 × 1.5 mm3 and is fabricated on FR4 substrate which has electrical permittivity of 4.3 and loss tangent of 0.025. FR4 is a low cost and easily available. The thickness of the substrate is 1.5 mm. The impedance bandwidth of the proposed antenna is 151.11 % (3.48 GHz to 25 GHz). The peak gain and radiation efficiency of the proposed antenna are 4.5 dB and 68 % respectively in the operating frequency band. Due to introduction of rings on the edges of the octagon and cutting of circular slots with the rectangular slits in the ground plane the antenna starts resonating from 3.48 GHz to 25 GHz. Simulated results are in good agreement with the measured results. The proposed antenna covers partial frequency range for ultra-wide band applications, 3.5/5.5 GHz WiMAX band, 5.2/5.8 GHz WLAN band, 8/12 GHz X-band, 12/18 GHz Ku -band. It can be used in space and satellite communications etc. Curves of radiation pattern and S-parameter of both simulated and measured results are shown. The impedance curves, surface current, radiation efficiency, simulated return losses, gain, and radiation patterns of the proposed antenna are described in the paper.
DOI: 10.6084/m9.figshare.7899803.v1
title: Survival benefits of interferon-based therapy in patients with recurrent hepatitis C after orthotopic liver transplantation

description: Recurrent hepatitis C after orthotopic liver transplantation (OLT) is universal and can lead to graft failure and, consequently, reduced survival. Hepatitis C treatment can be used to prevent these detrimental outcomes. The aim of this study was to describe rates of hepatitis C recurrence and sustained virological response (SVR) to interferon-based treatment after OLT and its relationship to survival and progression of liver disease through retrospective analysis of medical records of 127 patients who underwent OLT due to cirrhosis or hepatocellular carcinoma secondary to chronic hepatitis C between January 2002 and December 2013. Fifty-six patients were diagnosed with recurrent disease, 42 started interferon-based therapy and 37 completed treatment. Demographic, treatment- and outcome-related variables were compared between SVR and non-responders (non-SVR). There was an overall 54.1% SVR rate with interferon-based therapies. SVR was associated with longer follow-up after treatment (median 66.5 vs 37 months for non-SVR, P=0.03) and after OLT (median 105 vs 72 months, P=0.074), and lower rates of disease progression (15 vs 64.7%, P=0.0028) and death (5 vs 35.3%, P=0.033). Regardless of the result of therapy (SVR or non-SVR), there was a significant difference between treated and untreated patients regarding the occurrence of death (P&lt;0.001) and months of survival (P&lt;0.001). Even with suboptimal interferon-based therapies (compared to the new direct-acting antivirals) there is a 54.1% SVR rate to treatment. SVR is associated with improved survival and reduced risks of clinical decompensation, loss of the liver graft and death.
DOI: 10.6084/m9.figshare.7899791
title: Orthodontic traction of impacted canines: Concepts and clinical application

description: ABSTRACT Orthodontic traction of impacted canines stands as a major challenge for Orthodontics. It is a relatively frequent clinical complaint which management, more often than not, requires a multidisciplinary approach. Surgical exposure of the impacted canine, and the complex orthodontic mechanics applied to align the tooth back into the arch, may frequently lead to complications involving supporting tissues, not to mention the long treatment time and high costs imposed to patients. In face of that, it is worth highlighting the relevance of early diagnosis as to intervene efficiently and as soon as possible. This paper presents a review of the main concepts involving prevalence, etiology and classification of impacted canines, and describes the different clinical management options that would help solve the problem. It illustrates the topic by presenting a treatment delivered to a 13 year 7 months old male patient, suffering from a Class II, division 2, left subdivision, malocclusion, associated to a deep bite and a prolonged retention of a primary upper canine caused by the impaction of the permanent tooth. Corrective orthodontic therapy was associated to a rapid maxillary expansion and to the use of a high pull headgear. Impacted canine was submitted to orthodontic traction and correctly positioned back into the arch. This approach proved to be efficient in meeting both functional and aesthetic goals.
DOI: 10.6084/m9.figshare.7899794.v1
title: Decreased platelet responsiveness to clopidogrel correlates with CYP2C19 and PON1 polymorphisms in atherosclerotic patients

description: Clopidogrel and aspirin are the most commonly used medications worldwide for dual antiplatelet therapy after percutaneous coronary intervention. However, clopidogrel hyporesponsiveness related to gene polymorphisms is a concern. Populations with higher degrees of genetic admixture may have increased prevalence of clopidogrel hyporesponsiveness. To assess this, we genotyped CYP2C19, ABCB1, and PON1 in 187 patients who underwent percutaneous coronary intervention. Race was self-defined by patients. We also performed light transmission aggregometry with adenosine diphosphate (ADP) and arachidonic acid during dual antiplatelet therapy. We found a significant difference for presence of the CYP2C19*2 polymorphism between white and non-white patients. Although 7% of patients had platelet resistance to clopidogrel, this did not correlate with any of the tested genetic polymorphisms. We did not find platelet resistance to aspirin in this cohort. Multivariate analysis showed that patients with PON1 and CYP2C19 polymorphisms had higher light transmission after ADP aggregometry than patients with native alleles. There was no preponderance of any race in patients with higher light transmission aggregometry. In brief, PON1 and CYP2C19 polymorphisms were associated with lower clopidogrel responsiveness in this sample. Despite differences in CYP2C19 polymorphisms across white and non-white patients, genetic admixture by itself was not able to identify clopidogrel hyporesponsiveness.
DOI: 10.6084/m9.figshare.7899797.v1
title: Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements

description: IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven B-ALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of B-ALL, introduced for the first time in Tunisian laboratories.
DOI: 10.6084/m9.figshare.7899800.v1
title: Dumbbell Shaped Microstrip Broadband Antenna

description: Abstract In this article, a dumbbell shaped microstrip broadband microstrip antenna with partial ground plane is presented, The overall dimension of the proposed antenna is 20 × 15 × 1.5 mm3 and is fabricated on FR4 substrate which has electrical permittivity of 4.3 and loss tangent of 0.025. FR4 is a low cost and easily available. The thickness of the substrate is 1.5 mm. The impedance bandwidth of the proposed antenna is 151.11 % (3.48 GHz to 25 GHz). The peak gain and radiation efficiency of the proposed antenna are 4.5 dB and 68 % respectively in the operating frequency band. Due to introduction of rings on the edges of the octagon and cutting of circular slots with the rectangular slits in the ground plane the antenna starts resonating from 3.48 GHz to 25 GHz. Simulated results are in good agreement with the measured results. The proposed antenna covers partial frequency range for ultra-wide band applications, 3.5/5.5 GHz WiMAX band, 5.2/5.8 GHz WLAN band, 8/12 GHz X-band, 12/18 GHz Ku -band. It can be used in space and satellite communications etc. Curves of radiation pattern and S-parameter of both simulated and measured results are shown. The impedance curves, surface current, radiation efficiency, simulated return losses, gain, and radiation patterns of the proposed antenna are described in the paper.
DOI: 10.6084/m9.figshare.7899776
title: Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase

description: Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the extracellular signal-regulated kinase (ERK) signal pathway in a time-dependent manner. Inhibition of ERK by its inhibitor U0126 or by its specific small interfering RNA (siRNA) blocked the CaA-induced improvement in cell viability and the protective effects against H2O2-mediated DNA damage. This study adds to the understanding of the antioxidant effects of CaA by identifying a novel molecular mechanism of enhanced cell viability and protection against DNA damage.
DOI: 10.6084/m9.figshare.7899779
title: Teacher, Professional or Educator: the Conception of Special Education Teacher in the Academic Productions of the Specific Field of Special Education (2000-2016)

description: ABSTRACT: In this paper we intend to present a study on the production of knowledge regarding Special Education teachers in Brazil from 2000 to 2016. Our objective is to analyze how the conception about this particular kind of teacher is being disseminated through academic research. It is a bibliographic and qualitative research, theoretically and methodologically based on historical-dialectical materialism. We used a review of academic research as a methodological procedure for the collection and analysis of data, which contributed to the selection of academic papers and initial analysis of the concentration of these works by year, location, faculty and research groups. Within the 16 years covered by this research, we selected 24 works including theses, dissertations, scientific papers and papers published in event proceedings, which focused their analyses mainly on Special Education teachers. These works allowed us to highlight the imprecision of how this particular kind of teacher is named, which, to a large extent, indicates the conception of teacher hegemonically disseminated at the beginning of the 21st century. In the analysis of the selected papers, we highlighted three ways of naming the Special Education teacher: the teacher, the professional and the educator, which, in short, demonstrate an extended conception of teaching and the correlation of academic research with the proposal of the educational policy in force.
DOI: 10.6084/m9.figshare.7899788
title: Highly birefringent do-octagonal photonic crystal fibers with ultra flattened zero dispersion for supercontinuum generation

description: Abstract Photonic crystal fiber (PCF) structures with do-octagonal geometry have been studied. These do-octagonal PCF structure have smaller circular holes arranged in rhombic fashion at its centre. Moreover, these small holes are doped with materials like butanol, ethanol, methanol and propanol. Do-octagonal PCF structures doped with methanol and air filled structure report very high birefringence. Ultra flattened zero dispersion has been achieved by all the simulated structures. Besides, low confinement loss and large nonlinearity have also been reported. Numerical simulation for supercontinuum generation has been performed. Supercontinuum spectra obtained for peak power 1 kW, 2 kW, 5 kW and 10 kW are 650 nm, 950 nm, 1450 nm and 2050 nm respectively.
DOI: 10.6084/m9.figshare.7899776.v1
title: Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase

description: Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the extracellular signal-regulated kinase (ERK) signal pathway in a time-dependent manner. Inhibition of ERK by its inhibitor U0126 or by its specific small interfering RNA (siRNA) blocked the CaA-induced improvement in cell viability and the protective effects against H2O2-mediated DNA damage. This study adds to the understanding of the antioxidant effects of CaA by identifying a novel molecular mechanism of enhanced cell viability and protection against DNA damage.
DOI: 10.6084/m9.figshare.7899779.v1
title: Teacher, Professional or Educator: the Conception of Special Education Teacher in the Academic Productions of the Specific Field of Special Education (2000-2016)

description: ABSTRACT: In this paper we intend to present a study on the production of knowledge regarding Special Education teachers in Brazil from 2000 to 2016. Our objective is to analyze how the conception about this particular kind of teacher is being disseminated through academic research. It is a bibliographic and qualitative research, theoretically and methodologically based on historical-dialectical materialism. We used a review of academic research as a methodological procedure for the collection and analysis of data, which contributed to the selection of academic papers and initial analysis of the concentration of these works by year, location, faculty and research groups. Within the 16 years covered by this research, we selected 24 works including theses, dissertations, scientific papers and papers published in event proceedings, which focused their analyses mainly on Special Education teachers. These works allowed us to highlight the imprecision of how this particular kind of teacher is named, which, to a large extent, indicates the conception of teacher hegemonically disseminated at the beginning of the 21st century. In the analysis of the selected papers, we highlighted three ways of naming the Special Education teacher: the teacher, the professional and the educator, which, in short, demonstrate an extended conception of teaching and the correlation of academic research with the proposal of the educational policy in force.
DOI: 10.6084/m9.figshare.7899785
title: Physical stability of iron ore caves: geomechanical studies of a shallow underground cave in SE Brazil

description: Abstract Caves hosted in iron formations are attracting considerable interest due to their scientific and environmental value. Some of these caves are located in or near iron ore mine sites, which represent an important source of income to Brazil. However, the Brazilian legislation requires speleological studies that currently have an impact upon ore reserves and environmental licensing processes. The aim of this study was to apply conventional geotechnical empirical approaches to a cave located within the grounds of an iron ore mine in the Iron Quadrangle and validate it with numerical modelling, to ensure the method's applicability to natural caves under mining activity influence. These studies comprised the mapping of structural, geotechnical and geomechanical features of rocks hosting and surrounding the cave and to propose geotechnical domains. Field data collection covered the geotechnical parameters necessary to calculate the characteristic Mining Rock Mass Rating (MRMR) for each geotechnical domain, and to evaluate the stability conditions using the stability index or hydraulic radius of the cave. These geotechnical parameters were then used to calculate the physical parameters used in two-dimensional numerical simulations to verify the good stability conditions of the cave, corroborating the adequacy of Laubscher's diagram. The results of this study, although comprising only one cave, suggest that Laubscher's diagram is applicable for assessing the geotechnical behavior of iron ore caves.
DOI: 10.6084/m9.figshare.7899767
title: Evaluation of a father and son with atypical chronic myeloid leukemia with SETBP1 mutations and a review of the literature

description: We report the case of a father and son diagnosed with atypical chronic myeloid leukemia (aCML). Both patients harbored SETBP1 mutations, which are present in 24.3% of aCML patients. Moreover, both shared the variant encoding p.Pro737His, but the aCML severity was greater in the son because of the presence of two other missense mutations causing p.Asp868Asn and p.Ser885Arg alterations. SETBP1 mutations may be associated with an adverse prognosis, so their detection would help in the diagnosis of aCML and the determination of a patient's prognosis.
DOI: 10.6084/m9.figshare.7899770
title: Frequency and factors associated with Theileria equi, Babesia caballi and Trypanosoma evansi in equids from Bahia (Northeast Brazil)

description: Abstract The aim of this study was to determine the frequency and factors associated to Babesia caballi, Theileria equi and Trypanosoma evansi in naturally infected equids from the northeast Brazil. Blood samples from 569 equids (528 horses, 8 mules, and 33 donkeys) were collected and tested for the presence of DNA of each of these protozoan parasites by PCR. Generalized linear models were used to evaluate risk factors associated with the infection. The frequency of T. equi infection was 83.5% (475/569) - 84.3% in horses, and 73.2% in donkeys and mules. The results of the final model indicated that age (senior group) and animal species (mule and donkey group) were protective factors against this pathogen. The frequency of B. caballi infection was 24.3% (138/569) - 23.5% in horses and 34.1% in donkeys and mules. Age (adult and senior group) was considered a protective factor against B. caballi infection whereas animal species (donkey and mule group) were considered a risk factor for the infection. Trypanosoma evansi infection was not detected in any of animals. Our results suggest that equids from the area studied may be infected earlier in life with the etiological agents of equine piroplasmosis and become asymptomatic carriers.