DOI: 10.5061/dryad.h8j76
title: Data from: The rediscovery of a long described species reveals additional complexity in speciation patterns of poeciliid fishes in sulfide springs

description: The process of ecological speciation drives the evolution of locally
 adapted and reproductively isolated populations in response to divergent
 natural selection. In Southern Mexico, several lineages of the freshwater
 fish species of the genus Poecilia have independently colonized toxic,
 hydrogen sulfide-rich springs. Even though ecological speciation processes
 are increasingly well understood in this system, aligning the taxonomy of
 these fish with evolutionary processes has lagged behind. While some
 sulfide spring populations are classified as ecotypes of Poecilia
 mexicana, others, like P. sulphuraria, have been described as highly
 endemic species. Our study particularly focused on elucidating the
 taxonomy of the long described sulfide spring endemic, Poecilia thermalis
 Steindachner 1863, and investigates if similar evolutionary patterns of
 phenotypic trait divergence and reproductive isolation are present as
 observed in other sulfidic species of Poecilia. We applied a geometric
 morphometric approach to assess body shape similarity to other sulfidic
 and non-sulfidic fish of the genus Poecilia. We also conducted
 phylogenetic and population genetic analyses to establish the phylogenetic
 relationships of P. thermalis and used a population genetic approach to
 determine levels of gene flow among Poecilia from sulfidic and
 non-sulfidic sites. Our results indicate that P. thermalis' body
 shape has evolved in convergence with other sulfide spring populations in
 the genus. Phylogenetic analyses placed P. thermalis as most closely
 related to one population of P. sulphuraria, and population genetic
 analyses demonstrated that P. thermalis is genetically isolated from both
 P. mexicana ecotypes and P. sulphuraria. Based on these findings, we make
 taxonomic recommendations for P. thermalis. Overall, our study verifies
 the role of hydrogen sulfide as a main factor shaping convergent,
 phenotypic evolution and the emergence of reproductive isolation between
 Poecilia populations residing in adjacent sulfidic and non-sulfidic
 environments.
description: Microsatellite
 dataThe excel files contains
 all raw data from population genetic analyses as described in the paper.
 Specimens are grouped by collection site (as per Table 2 in the main
 manuscript). Microsatellite loci are grouped by multiplex reactions. Locus
 names follow Slattery et al. (2012): Twelve new microsatellite loci for
 the sulphur molly (Poecilia sulphuraria) and the related Atlantic molly
 (P. mexicana). Conservation Genetics Resources 4 (4):
 935-937.microsatellite
 data.xlsBody shape data for the
 lateral projectionThe file contains the
 aligned landmark coordinates for body shape in the lateral projection. For
 each individual, we also provide information on the species designation,
 collection location, drainage, whether the habitat is sulfidic or not,
 sex, and centroid size.Final dataset
 lateral.xlsxBody shape data for the
 dorsal projectionThe file contains the
 aligned landmark coordinates for body shape in the dorsal projection. For
 each individual, we also provide information on the species designation,
 collection location, drainage, whether the habitat is sulfidic or not,
 sex, and centroid size.Final dataset
 dorsal.xlsxBody shape data for the
 lateral projection for historical syntypes of P. thermalis and
 contemporary Poecilia from the same drainageThe file contains the
 aligned landmark coordinates for body shape in the lateral projection. For
 each individual, we also provide information on the species designation,
 collection location, drainage, whether the habitat is sulfidic or not,
 sex, centroid size, and whether the sample is from a historical museum or
 a contemporary collection.Final dataset
 museum.xlsx