DOI: 10.5061/dryad.7qh1r
title: Data from: Tomato Sl3-MMP, a member of the Matrix metalloproteinase family, is required for disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000

description: Background: Matrix metalloproteinases (MMPs) are a family of
 zinc-dependent endopeptidases. MMPs have been characterized in detail in
 mammals and shown to play key roles in many physiological and pathological
 processes. Although MMPs in some plant species have been identified, the
 function of MMPs in biotic stress responses remains elusive. Results: A
 total of five MMP genes were identified in tomato genome. qRT-PCR analysis
 revealed that expression of Sl-MMP genes was induced with distinct
 patterns by infection of Botrytis cinerea and Pseudomonas syringae pv.
 tomato (Pst) DC3000 and by treatment with defense-related hormones such as
 salicylic acid, jasmonic acid and ethylene precursor 1-amino
 cyclopropane-1-carboxylic acid. Virus-induced gene silencing (VIGS)-based
 knockdown of individual Sl-MMPs and disease assays indicated that
 silencing of Sl3-MMP resulted in reduced resistance to B. cinerea and Pst
 DC3000, whereas silencing of other four Sl-MMPs did not affect the disease
 resistance against these two pathogens. The Sl3-MMP-silenced tomato plants
 responded with increased accumulation of reactive oxygen species and
 alerted expression of defense genes after infection of B. cinerea.
 Transient expression of Sl3-MMP in leaves of Nicotiana benthamiana led to
 an enhanced resistance to B. cinerea and upregulated expression of
 defense-related genes. Biochemical assays revealed that the recombinant
 mature Sl3-MMP protein had proteolytic activities in vitro with distinct
 preferences for specificity of cleavage sites. The Sl3-MMP protein was
 targeted onto the plasma membrane of plant cells when transiently
 expressed in onion epidermal cells. Conclusion: VIGS-based knockdown of
 Sl3-MMP expression in tomato and gain-of-function transient expression of
 Sl3-MMP in N. benthamiana demonstrate that Sl3-MMP functions as a positive
 regulator of defense response against B. cinerea and Pst DC3000.
description: Protein sequences used
 in phylogenetic tree analysis of Sl-MMPs with other plant
 MMPsPhylogenetic tree was
 constructed by Neighbor-joining method using MEGA program. Plant MMPs used
 and their GenBank accessions are as follows: Arabidopsis thaliana At1-MMP
 (NP_193397), At2-MMP (NP_177174), At3-MMP (NP_173824), At4-MMP
 (NP_182030), At5-MMP (NP_176205), Glycine max Gm2-MMP (AAL27029),
 Gm-ACU24527 (ACU24527), Gm-Slti114 (ABW96008), Hordeum vulgare HvMMP1
 (BAJ94792), HvMMP2 (BAJ93963), HvMMP3 (BAJ94176), HvMMP4 (BAJ90264),
 Medicago truncatula Mt1-MMP (CAA77093), Nicotiana tabacum NtMMP1
 (ABF58910), Solanum lycopersicum Sl1-MMP (XP_010325488), Sl2-MMP
 (CCH68443), Sl3-MMP (NP_001266203), Sl4-MMP (XP_010320628), Sl5-MMP
 (XP_004248606), Zea mays ZmMMP1 (NP_001151749), ZmMMP2 (NP_001142095),
 Oryza sativa Os1-MMP (NP_001048075), Os2-MMP (NP_001057259), Os3-MMP
 (NP_001065361).SlMMP Tree
 final.fastaPhylogenetic tree
 analysis of Sl-MMPs with other plant MMPs.Phylogenetic tree was
 constructed by Neighbor-joining method using MEGA
 program.Tree.jpg