cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.qdvprh
title: Occurrence Download

description: A dataset containing 1616503 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Pl@ntNet observations"
 ]
}

 The dataset includes 1616503 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0193923-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.hqypcn
title: Occurrence Download

description: A dataset containing 7692354 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Specimen)",
 "Elevation is greater than or equal to 0.0m",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Issue is one of (Coordinate rounded, Geodetic datum assumed WGS84, Coordinate reprojected)",
 "IucnRedListCategory is one of (EX, EW, CR, EN, VU, NT, LC)",
 "OccurrenceStatus is Present",
 "TaxonKey is Plantae"
 ]
}

 The dataset includes 7692354 records from 1246 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0176245-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.18112/openneuro.ds006131.v1.0.0
title: PAFIN: PennLINC AFfective INstability
DOI: 10.48597/sy2f-rcvc
title: Measurement of Nitrogen_dioxide at Östad

description: Measurement of Nitrogen_dioxide at Östad
DOI: 10.48597/wqnb-w2gr
title: Measurement of Nitrogen_dioxide at Östad

description: Measurement of Nitrogen_dioxide at Östad
DOI: 10.6084/m9.figshare.29127098
title: Data for article "<i>From molecules to nanoplastics SERS detection: new insights into the role of analyte on the plasmonic substrate design</i>"

description: Reflectance spectra of AuFoN nanostructures; SERS spectra of benzenthiol on AuFoN nanostructures.
DOI: 10.25399/unisadata.29127251
title: Skills and competencies of academic librarians to use information technology tools in the digital era : a systematic literature review

description: The systematic review analyzed 27 works of literature related to the skills and competencies needed by academic librarians to use information technology (IT) tools effectively in the digital age. The review is carried out following the recommended Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. Relevant literature was extracted from eight major academic databases, including EBSCOhost, Emerald Insight, Scopus, Sage Journal, Web of Science, Taylor and Francis, ScienceDirect, and Google Scholar. The retrieved articles were exported to RefWorks to identify duplicates and screen articles meeting the inclusion criteria. Only articles that are empirical in nature, published in a peer-reviewed journal, written in English, published between 2020 and 2024 with affiliation from five countries (Nigeria, South Africa, Pakistan, India and the USA) and addressing either one or all the objectives of this study were used. The study determined various IT tools used in academic libraries to improve service delivery in terms of efficiency, accessibility, and quality. This trend requires librarians to develop advanced digital skills and competencies to cope with the digital world. The study also identified challenges encountered by academic libraries regarding the implemented IT tools. The recommendations based on the findings were outlined.
DOI: 10.25399/unisadata.29127251.v1
title: Skills and competencies of academic librarians to use information technology tools in the digital era : a systematic literature review

description: The systematic review analyzed 27 works of literature related to the skills and competencies needed by academic librarians to use information technology (IT) tools effectively in the digital age. The review is carried out following the recommended Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. Relevant literature was extracted from eight major academic databases, including EBSCOhost, Emerald Insight, Scopus, Sage Journal, Web of Science, Taylor and Francis, ScienceDirect, and Google Scholar. The retrieved articles were exported to RefWorks to identify duplicates and screen articles meeting the inclusion criteria. Only articles that are empirical in nature, published in a peer-reviewed journal, written in English, published between 2020 and 2024 with affiliation from five countries (Nigeria, South Africa, Pakistan, India and the USA) and addressing either one or all the objectives of this study were used. The study determined various IT tools used in academic libraries to improve service delivery in terms of efficiency, accessibility, and quality. This trend requires librarians to develop advanced digital skills and competencies to cope with the digital world. The study also identified challenges encountered by academic libraries regarding the implemented IT tools. The recommendations based on the findings were outlined.
DOI: 10.6075/j02f7m30
title: CIL:37174, Bufo marinus, epithelial cell. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x112,000.
description: Transmission electron micrograph of two frog epithelial cell cells showing the tight junction complex running horizontally in this image. Additional reference: Farquhar, M.G. and G.E. Palade. 1965. Cell junctions in amphibian skin. J. Cell Biol. 26:263-291. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0vt1qw6
title: CIL:10851, Homo sapiens, neutrophilic metamyelocyte. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Data qualification: Processed
description: Figure 276 from Chapter 8 (Lysosomes) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. An early myelocyte from human bone marrow with numerous azurophil granules with cytochemical properties of lysosomes. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
DOI: 10.6075/j0ht2nqd
title: CIL:45553, Homo sapiens, dendritic cell. In Cell Image Library

description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: phalloidin; 4',6-diamidino-2-phenylindole (DAPI); TagGFP
description: Imaging an HIV infected dendritic cell reveals numerous viral particles at the tips of filopodia. Actin-based membrane extensions from the dendritic cell are phallodin stained (red). HIV particles (white) decorate the filopodial terminal ends. To label viral particles, a construct containing eGFP at the C-terminus of HIV matrix was expressed. The nucleus of the cell is stained with DAPI and is represented in cyan.
DOI: 10.6075/j0q23zc9
title: CIL:12931, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 11,500 X. See Ladinsky and Howell (2007) for more information on methods used.
DOI: 10.6075/j0z037r1
title: CIL: 52782, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given tile position
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: Tile x=1, y=1, z=1 from PS11_167511L_Myc_Terc
DOI: 10.6075/j0xd10dq
title: CIL:13717, Homo sapiens, endothelial cell. In Cell Image Library

description: YFP-WT PINK1 (green) shows mitochondrial localization in HeLa cells following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are unpermeabilized (not treated with detergent), antibodies to GFP (red) (used to identify YFP-WT PINK1), and Tom20 (white), a mitochondrial outer membrane marker, are unable to reach and bind their epitopes. YFP-WT PINK1 transfected HeLa cells were treated with CCCP (10µM) for 3 hrs, fixed with 4% paraformaldehyde and not permeabilized. Primary antibodies used were: anti-GFP polyclonal Ab (Invitrogen) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4c, top row, and is a control experiment for a differential permeabilization assay to determine submitochondrial localization of PINK1 and that is further described in Fig 4c of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EYFP; primary antibody plus labeled secondary antibody
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0pk0g7f
title: CIL:39544, Saccharomyces cerevisiae. In Cell Image Library

description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP taggedMAD2. 
 
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, mad2 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad2p forms a tight complex with another spindle checkpoint protein, Mad1p, throughout the cell cycle. Mad2p also forms a complex with Cdc20p, which activates the anaphase promoting complex, and Mad3p; the presence of Mad1p is required for the complex to form. Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and the protein kinase Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation. Homologs of Mad2p act in the spindle checkpoint in Xenopus (Xmad2) and human (MAD2L1 andMAD2L2.MAD2 was originally identified as the open reading frame YJL031C. However, it is now known thatMAD2 corresponds to the adjacent open reading frame YJL030W, while YJL031C encodes the geranylgeranyltransferase subunit Bet4p.
 
These phase,gfp z-series images are part of an image group that ranges from CIL:39541-39545. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
DOI: 10.6075/j0833rqm
title: CIL: 53680, Homo sapiens, HeLa. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: CMGC family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
description: Visualization methods: confocal microscopy
DOI: 10.6075/j0n29vpr
title: CIL:19118, Spiroplasma kunkelii, Dalbulus maidis, prokaryotic cell. In Cell Image Library

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the anterior and posterior midgut (amg and pmg). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12580, from the same image group, is an uncropped unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005)
Ann. Entomol. Soc. Am. 98(6): 820Ð826.
description: Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Data qualification: Processed
DOI: 10.6075/j02f7mmq
title: CIL:18491, damselfly, flight muscle cell. In Cell Image Library

description: Most images show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. This image is part of the group image CFA00512. TEM magnifications 24400X. The negative film was scanned at LBL then reduced in size for upload. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer. pH 7.2. stored at 4C for several days. Muscle dissected out. Infiltrated in 30% glycerol in water. Frozen in liquid nitrogen cooled Freon, fractured in Balzers 400 Freeze-fracture, shadowed with platinum at 45o angle. Replicas were cleaned with Clorox (to remove tissue and with either sulfuric acid or NaOH to try removing the tracheolae. Replicas examined in a Philips 410 Microscope. Reference for these images: 
Takekura, H. and Franzini-Armstrong C. The structure of Ca2+ release units in arthropod body muscle indicates an indirect mechanism for excitation-contraction coupling. Biophys. J. 83, 83, 2742-2753, 2002.
description: Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: unembedded tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: digitized negative film
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0n29vn9
title: CIL:19122, Spiroplasma kunkelii, Dalbulus maidis, prokaryotic cell, secretory cell. In Cell Image Library

description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the principal salivary gland (psg). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12584, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005)
Ann. Entomol. Soc. Am. 98(6): 820Ð826.
description: Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Data qualification: Processed
DOI: 10.6075/j0dr2t64
title: CIL:19121, Spiroplasma kunkelii, Dalbulus maidis, fat cell, prokaryotic cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Data qualification: Processed
description: Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing fat tissue (ft) and labeled nuclei (n). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:12583, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005)
Ann. Entomol. Soc. Am. 98(6): 820Ð826.