cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/36x-f2c-smv
title: Glaucopsyche alexis (Poda, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.30489610.v1
title: Characterization of wound-induced electrical signals and reactive oxygen species in chickpea <i>(Cicer arietinum)</i>

description: Mechanical damage to plants triggers both localized and systemic responses that activate plant defense mechanisms. Early signaling events include calcium (Ca<sup>2+</sup>) flux, reactive oxygen species (ROS), and electrical alterations. These signals coordinate downstream defense pathways, enabling plant acclimation to biotic stress. Electrical signaling following wounding/herbivory has been extensively studied in <i>Arabidopsis</i>; however, its dynamics in crop plants such as chickpea (<i>Cicer arietinum</i>) are not well understood. The pattern of the SWP in chickpea was similar to that in <i>Arabidopsis</i> but with a longer repolarization phase and was detectable only within the leaflets. The signals generated by damaging the leaflet were more pronounced, propagated bidirectionally and varied between herbivore-susceptible and tolerant chickpea varieties. The SWP duration is correlated with increased expression of <i>AOS</i> and <i>OPR3</i> transcripts, which are markers of the stress hormone JA. Additionally, ROS production in wounded chickpea leaflets is associated with increased expression of ROS-generating genes. The use of DPI, an inhibitor of NADPH oxidase, which is responsible for ROS production, inhibited SWP, suggesting the crucial role of ROS in wound-induced SWP. This study provides insight into the interplay between wound-induced electrical signaling and ROS production in chickpea and proposes the measurement of electrical signals as a rapid, noninvasive approach for screening crop cultivars for pest susceptibility and tolerance. Electrical signaling in chickpea follows the SWP pattern in response to wounding.SWPs propagate bidirectionally with amplitude and propagation dependent on wound intensity.SWP duration varies between herbivore-susceptible and -tolerant chickpea varieties.Wounding triggers localized ROS accumulation in chickpea leaflets.DPI attenuated SWP duration, highlighting the interdependence of ROS and electrical signaling. Electrical signaling in chickpea follows the SWP pattern in response to wounding. SWPs propagate bidirectionally with amplitude and propagation dependent on wound intensity. SWP duration varies between herbivore-susceptible and -tolerant chickpea varieties. Wounding triggers localized ROS accumulation in chickpea leaflets. DPI attenuated SWP duration, highlighting the interdependence of ROS and electrical signaling.
DOI: 10.3535/jrv-hep-p6x
title: Cyaniris semiargus (von Rottemburg, 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.28083224.v1
title: Expression characteristics of <i>CsESA1</i> in citrus and analysis of its interacting protein

description: The most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by ‘<i>Candidatus Liberibacter</i> asiaticus’ (<i>Ca</i>Las). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to <i>Ca</i>Las infection induction were screened, and one gene cloned with higher differential expression level was selected and named <i>CsCESA1</i>. we verified the interaction between CsCESA1 and citrus exopolysaccharide 2 (CsEPS2) proteins. Subcellular localization in tobacco indicated that both CsCESA1 and CsEPS2 proteins are primarily located in the nucleus and cytoplasm. RT-qPCR analysis indicated that the expression levels of <i>CsCESA1</i> and <i>CsEPS2</i> were associated with variety tolerance, tissue site, and symptom development. Furthermore, we generated <i>CsCESA1</i> and <i>CsEPS2</i> silencing plants and obtained <i>CsCESA1</i> and <i>CsEPS2</i> silencing and overexpressing hairy roots. The analysis of hormone content and gene expression also showed that <i>CsCESA1</i> and <i>CsEPS2</i> are involved in transcriptional regulation of genes involved in systemic acquired resistance (SAR) response. In conclusion, our results suggested that <i>CsCESA1</i> and <i>CsEPS2</i> could serve as potential resistance genes for HLB disease, offering insights into the plant’s defense mechanisms against HLB.
DOI: 10.3535/efg-tcb-qaj
title: Cyaniris semiargus (von Rottemburg, 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.29041062
title: Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis in <i>Bupleurum chinense</i> DC

description: <i>Bupleurum chinense</i> DC. a medicinal plant valued for saikosaponins (SSs) with antipyretic and hepatoprotective properties, faces constrained SS biosynthesis mediated by abscisic acid (ABA) during growth. Basic helix-loop-helix (bHLH) transcription factors (TFs) are hypothesized to participate in ABA signaling cascades, but their mechanistic role in SS regulation remains undefined. In this study, 20 differentially expressed <i>BcbHLH</i> genes were identified by transcriptomic profiling of ABA-induced hairy roots, with four MYC-family candidates (<i>BcbHLH1-BcbHLH4</i>) demonstrating ABA-responsive regulatory potential. ABA exposure (100 or 200 μmol/L, 24–72 h) induced dose-dependent SS reduction, while correlation analyses revealed coordinated expression between <i>BcbHLH1-BcHMGR</i> (<i>r</i> = 0.62) and <i>BcbHLH4-BcBAS</i> (<i>r</i> = 0.78), pinpointing these TFs as critical nodes in SS pathway modulation. Tissue-specific profiling showed predominant <i>BcbHLH</i> expression in stems and young leaves, with nuclear localization confirming their transcriptional regulatory organelles. BcbHLH3/4 exhibited transcriptional activation activity in the MYC_N domain, while molecular docking predicted 11th Arginine in the HLH domain as essential for G-box DNA binding. Collectively, our findings suggest that BcbHLH1-BcbHLH4 may serve as potential switches for fine-tuning ABA responsiveness in SS biosynthesis. Strategic manipulation of <i>BcbHLH</i> activity through genetic engineering approaches such as CRISPR-based editing or overexpression could alleviate ABA-mediated biosynthetic repression. Furthermore, precision engineering of the critical functional domain in BcbHLH could enhance promoter-binding activity to target genes and improve SS biosynthesis efficiency. These findings provide a reference framework for harnessing transcriptional regulators to optimize SS production in <i>Bupleurum chinense</i> DC.
DOI: 10.6084/m9.figshare.29041062.v1
title: Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis in <i>Bupleurum chinense</i> DC

description: <i>Bupleurum chinense</i> DC. a medicinal plant valued for saikosaponins (SSs) with antipyretic and hepatoprotective properties, faces constrained SS biosynthesis mediated by abscisic acid (ABA) during growth. Basic helix-loop-helix (bHLH) transcription factors (TFs) are hypothesized to participate in ABA signaling cascades, but their mechanistic role in SS regulation remains undefined. In this study, 20 differentially expressed <i>BcbHLH</i> genes were identified by transcriptomic profiling of ABA-induced hairy roots, with four MYC-family candidates (<i>BcbHLH1-BcbHLH4</i>) demonstrating ABA-responsive regulatory potential. ABA exposure (100 or 200 μmol/L, 24–72 h) induced dose-dependent SS reduction, while correlation analyses revealed coordinated expression between <i>BcbHLH1-BcHMGR</i> (<i>r</i> = 0.62) and <i>BcbHLH4-BcBAS</i> (<i>r</i> = 0.78), pinpointing these TFs as critical nodes in SS pathway modulation. Tissue-specific profiling showed predominant <i>BcbHLH</i> expression in stems and young leaves, with nuclear localization confirming their transcriptional regulatory organelles. BcbHLH3/4 exhibited transcriptional activation activity in the MYC_N domain, while molecular docking predicted 11th Arginine in the HLH domain as essential for G-box DNA binding. Collectively, our findings suggest that BcbHLH1-BcbHLH4 may serve as potential switches for fine-tuning ABA responsiveness in SS biosynthesis. Strategic manipulation of <i>BcbHLH</i> activity through genetic engineering approaches such as CRISPR-based editing or overexpression could alleviate ABA-mediated biosynthetic repression. Furthermore, precision engineering of the critical functional domain in BcbHLH could enhance promoter-binding activity to target genes and improve SS biosynthesis efficiency. These findings provide a reference framework for harnessing transcriptional regulators to optimize SS production in <i>Bupleurum chinense</i> DC.
DOI: 10.6084/m9.figshare.30653200
title: <i>CERK1</i> is required for chitin-triggered reactive oxygen species generation in melon and is broadly conserved in cucurbits

description: CHITIN ELICITOR RECEPTOR KINASE 1 (<i>CERK1</i>), originally identified in <i>Arabidopsis thaliana</i>, encodes a pattern recognition receptor that perceives the fungal cell wall component called chitin to activate immune responses, including the production of reactive oxygen species (ROS) against fungal pathogens. Functional <i>CERK1</i> orthologs have been identified in plants, such as tomato, rice, and wheat. However, the knowledge of chitin-triggered immunity in Cucurbitaceae plants is currently limited. This study revealed that chitin triggers ROS generation in melon (<i>Cucumis melo</i>) and cucumber (<i>Cucumis sativus</i>), indicating that chitin is recognized by cucurbits. A subsequent homology search using the Arabidopsis CERK1 sequence identified CERK1 ortholog candidates of melon (CmCERK1) and cucumber (CsCERK1). Virus-induced gene silencing of <i>CmCERK1</i> severely reduced chitin-triggered ROS generation in melon, indicating that <i>CmCERK1</i> is essential for chitin recognition and the subsequent immune response. Genomic PCR of <i>CmCERK1</i> and ROS assay upon chitin treatment in multiple melon commercial cultivars also showed that functional <i>CmCERK1</i> is conserved in all the tested cultivars. Further analysis of the available genomes of various cucurbit plants suggested that <i>CERK1</i> is broadly conserved in cucurbit plants.
DOI: 10.3535/6a1-61f-jan
title: Cucullia umbratica (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.30882009
title: Unraveling alfalfa molecular adaptation to salinity and <i>Ascochyta medicaginicola</i> infection: insights from differential physiological traits and proteomics profiling

description: In real-life scenarios, plants are often exposed to biotic and abiotic stresses simultaneously; it is crucial to understand how they respond to single and combined stresses. This study explored the physiological and molecular responses of two contrasting alfalfa varieties, Gabes-2353 (tolerant) and Magna-601 (sensitive), to salinity and/or <i>Ascochyta medicaginicola</i> infection. Both varieties exhibited reductions in stem length, leaf relative water content, and chlorophyll content, with a greater decrease in Magna-601. Label-free LC‒MS/MS analysis identified 128 differentially abundant proteins (DAPs) between the two varieties, of which 14, 60, and 70 proteins were differentially regulated under salinity, Pm8 infection, and combined stresses, respectively. Gene Ontology analyses revealed that Gabes-2353 activated photosynthesis, metabolism, redox balance, and immune pathways, while Magna-601 disrupted carbohydrate metabolism. Pm8 infection induced immune pathways in both varieties, with antioxidant and metabolic proteins expressed in Gabes-2353, while protease inhibitors and defense proteins were expressed in Magna-601. Under combined stress, Gabes-2353 upregulated proteins associated with synergistic integration of metabolic and immune pathways. In contrast, Magna-601 activated broad stress-signaling responses but inhibited primary metabolism, aminoacyl-tRNA biosynthesis, and vitamin metabolism. The PPI and KEGG analyses identified 7-O-methyltransferase as a central hub in Gabes-2353, while Magna-601’s PPI network focused on starch mobilization, reflecting differential adaptation strategies.
DOI: 10.6084/m9.figshare.30882009.v1
title: Unraveling alfalfa molecular adaptation to salinity and <i>Ascochyta medicaginicola</i> infection: insights from differential physiological traits and proteomics profiling

description: In real-life scenarios, plants are often exposed to biotic and abiotic stresses simultaneously; it is crucial to understand how they respond to single and combined stresses. This study explored the physiological and molecular responses of two contrasting alfalfa varieties, Gabes-2353 (tolerant) and Magna-601 (sensitive), to salinity and/or <i>Ascochyta medicaginicola</i> infection. Both varieties exhibited reductions in stem length, leaf relative water content, and chlorophyll content, with a greater decrease in Magna-601. Label-free LC‒MS/MS analysis identified 128 differentially abundant proteins (DAPs) between the two varieties, of which 14, 60, and 70 proteins were differentially regulated under salinity, Pm8 infection, and combined stresses, respectively. Gene Ontology analyses revealed that Gabes-2353 activated photosynthesis, metabolism, redox balance, and immune pathways, while Magna-601 disrupted carbohydrate metabolism. Pm8 infection induced immune pathways in both varieties, with antioxidant and metabolic proteins expressed in Gabes-2353, while protease inhibitors and defense proteins were expressed in Magna-601. Under combined stress, Gabes-2353 upregulated proteins associated with synergistic integration of metabolic and immune pathways. In contrast, Magna-601 activated broad stress-signaling responses but inhibited primary metabolism, aminoacyl-tRNA biosynthesis, and vitamin metabolism. The PPI and KEGG analyses identified 7-O-methyltransferase as a central hub in Gabes-2353, while Magna-601’s PPI network focused on starch mobilization, reflecting differential adaptation strategies.
DOI: 10.34740/kaggle/dsv/14208704
title: Trending Youtube Video Statistics (113 Countries)
DOI: 10.3535/1a9-h6b-ye9
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.29859224
title: Rhizosphere microbial diversity and functional roles in tea cultivars: insights from high-throughput sequencing and functional isolates

description: Rhizosphere microorganisms play a significant role in influencing the growth and quality of tea plants (<i>Camellia sinensis</i>). However, the complex mechanisms underlying the interactions between rhizosphere microorganisms and tea plants require further investigation. In this study, we employed high-throughput sequencing and the isolation of functional rhizosphere microorganisms to examine variations in rhizosphere microbial diversity and functional characteristics among five distinct tea cultivars: <i>Camellia sinensis</i> cv. Wuniuzao, Fudingdahao, Fuyunliuhao, Jinxuan, and Fudingdabai, each recognized for its unique qualities and adaptability. Our results revealed significant differences in the community diversity of rhizosphere microorganisms among the different tea cultivars. The phylum <i>Mucoromycota</i> may exert a notable influence on the growth of cultivars Wuniuzao, Fudingdahao, and Fuyunliuhao through metabolic pathways such as lipid metabolism. Specifically, <i>Serratia</i> spp. and <i>Enterobacter</i> spp. which produce higher levels of IAA and were isolated from the rhizosphere soils of cultivars Wuniuzao and Fudingdahao, may play a critical role in promoting tea plant growth and development. Additionally, bacteria from the phylum <i>Acidobacteriota</i> may also contribute significantly to tea plant growth. These findings provide valuable insights into the roles of rhizosphere microorganisms in influencing the growth and quality of tea plants, offering a foundation for further exploration of microbial-assisted strategies to enhance tea cultivation.
DOI: 10.3535/6sc-7p2-0tz
title: Ochropacha duplaris (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.28108024
title: Do strigolactones play a role in the ascent and attachment behavior of <i>Pisum sativum</i>?

description: Strigolactones (SLs) are signaling compounds made by plants. They play a crucial role in acting as long-distance signals from root to shoot to coordinate shoot growth with root environmental conditions. Here, we test whether and how SLs play a role in the climbing behavior of pea plants by studying the circumnutation of the tendrils using three-dimensional (3D) kinematical analysis. To assess this, we compare the typical behavior of <i>P. sativum</i>, a wild-type plant that produces and perceives SLs, with mutants defective in SLs synthesis or signaling, known as <i>ramosus</i>(<i>rms</i>) mutants. The results indicate that mutant plants seem unable to locate and grasp a potential support. Their movement appears to be disoriented and much less energized. We contend that this research opens new avenues for exploring SLs’ role in plant behavior, a novel lens through which the role of SLs in root-to-shoot communication can be observed and analyzed.
DOI: 10.6084/m9.figshare.28108024.v2
title: Do strigolactones play a role in the ascent and attachment behavior of <i>Pisum sativum</i>?

description: Strigolactones (SLs) are signaling compounds made by plants. They play a crucial role in acting as long-distance signals from root to shoot to coordinate shoot growth with root environmental conditions. Here, we test whether and how SLs play a role in the climbing behavior of pea plants by studying the circumnutation of the tendrils using three-dimensional (3D) kinematical analysis. To assess this, we compare the typical behavior of <i>P. sativum</i>, a wild-type plant that produces and perceives SLs, with mutants defective in SLs synthesis or signaling, known as <i>ramosus</i>(<i>rms</i>) mutants. The results indicate that mutant plants seem unable to locate and grasp a potential support. Their movement appears to be disoriented and much less energized. We contend that this research opens new avenues for exploring SLs’ role in plant behavior, a novel lens through which the role of SLs in root-to-shoot communication can be observed and analyzed.
DOI: 10.6084/m9.figshare.29859224.v1
title: Rhizosphere microbial diversity and functional roles in tea cultivars: insights from high-throughput sequencing and functional isolates

description: Rhizosphere microorganisms play a significant role in influencing the growth and quality of tea plants (<i>Camellia sinensis</i>). However, the complex mechanisms underlying the interactions between rhizosphere microorganisms and tea plants require further investigation. In this study, we employed high-throughput sequencing and the isolation of functional rhizosphere microorganisms to examine variations in rhizosphere microbial diversity and functional characteristics among five distinct tea cultivars: <i>Camellia sinensis</i> cv. Wuniuzao, Fudingdahao, Fuyunliuhao, Jinxuan, and Fudingdabai, each recognized for its unique qualities and adaptability. Our results revealed significant differences in the community diversity of rhizosphere microorganisms among the different tea cultivars. The phylum <i>Mucoromycota</i> may exert a notable influence on the growth of cultivars Wuniuzao, Fudingdahao, and Fuyunliuhao through metabolic pathways such as lipid metabolism. Specifically, <i>Serratia</i> spp. and <i>Enterobacter</i> spp. which produce higher levels of IAA and were isolated from the rhizosphere soils of cultivars Wuniuzao and Fudingdahao, may play a critical role in promoting tea plant growth and development. Additionally, bacteria from the phylum <i>Acidobacteriota</i> may also contribute significantly to tea plant growth. These findings provide valuable insights into the roles of rhizosphere microorganisms in influencing the growth and quality of tea plants, offering a foundation for further exploration of microbial-assisted strategies to enhance tea cultivation.
DOI: 10.6084/m9.figshare.29107049.v1
title: <i>Target of Rapamycin</i> is involved in root hair development in <i>Phaseolus vulgaris</i>

description: Root hairs are essential for nutrient acquisition and rhizosphere interactions in vascular plants. While the Target of Rapamycin (TOR) kinase is a well established regulator of growth and metabolism, its role in root hair development in <i>Phaseolus vulgaris</i> remains underexplored. In this study, we investigated the role of TOR in root hair morphogenesis using RNA interference (RNAi)-mediated downregulation of <i>PvTOR</i> and transcriptomic profiling. Microscopic examination of <i>PvTOR</i>-RNAi roots confirmed significant reductions in root hair length and density. Transcriptomic analysis revealed differential expression of 148 <i>P. vulgaris</i> homologs of <i>Arabidopsis thaliana</i> root hair-related genes, with 63 genes downregulated and 85 upregulated. Gene Ontology enrichment analysis indicated that these differentially expressed genes (DEGs) were primarily involved in cellular development, cell differentiation, and redox regulation. Upregulation of phosphoinositide metabolism genes, ROS generators, and cell wall-related extensins suggests compensatory tip growth responses under TOR suppression. On the otherhand, repression of key auxin signaling genes and cell wall-loosening proteins such as <i>EXPA1</i> and <i>ENDOGLUCANASE5</i> indicates a shift away from elongation processes. Protein – protein interaction network analysis highlighted phosphoinositide and ROP GTPase signaling hubs as major pathways affected by TOR inhibition, suggesting that TOR indirectly modulates cell polarity and membrane dynamics essential for root hair development. These findings provide further evidence of TOR as a central integrator of hormonal, metabolic, and structural cues during root hair formation.
DOI: 10.3535/71x-7dc-s8c
title: Agriphila tristellus [Denis & Schiffermüller], 1775

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.6084/m9.figshare.30610373
title: Comparative analysis of chloroplast genomes and transcriptomics reveals the adaptation of <i>Glycyrrhiza</i> to salt stress

description: <i>Glycyrrhiza</i> is a perennial leguminous plant with salt tolerance, and its roots and rhizomes possess extremely significant medicinal value. The proper salinity can facilitate the growth of <i>Glycyrrhiza</i> and increase its content of medicinal ingredients. However, excessive salinity can negatively affect growth and medicinal component contents. The salt tolerance mechanism has not yet been fully elucidated, especially the information of chloroplast genome is in short supply. Present research investigated the genetic diversity of <i>Glycyrrhiza</i> by conducting comparative genomic, adaptive evolutionary, haplotype, population structure, and phylogenetic analyses of the chloroplast genes. Transcriptome analysis revealed that the chloroplast genes of different <i>Glycyrrhiza</i> varieties respond to salt stress at different stages and that these responsive genes are associated predominantly with the photosynthetic structure and regulation of protein synthesis. The editing efficiency of the psbA and rrn23 genes increases significantly under salt stress, potentially contributing to plant adaptation. In summary, analysis of the chloroplast genome provided valuable insights into the genetic diversity and phylogenetic relationships of <i>Glycyrrhiza</i>. Furthermore, transcriptomic data supplemented existing knowledge on chloroplast-mediated salt tolerance mechanisms, offering a foundation for future investigations into the adaptive strategies of <i>Glycyrrhiza</i> under saline conditions.