DOI: 10.5061/dryad.sn02v6x2w
title: The effects of intestinal dendritic cells on the imbalance of mucosal Th1/Th2 immune response pathway and visceral hypersensitivity in IBS-D rats

description: To study the role of intestinal dendritic cells in the imbalance
 of local mucosal Th1/Th2 immune response pathway and its effect
 on visceral hypersensitivity in IBS-D rats through the synergy effects by
 CD4+Th cells and mast cells. Methods: 45 adult SD rats
 were randomly divided into a blank control group, model control group,
 and  model group,each group (n = 15). Rats in the model group
 were subjected to IBS visceral hypersensitive modeling by acetic acid
 enema combined with restraint stress. AWR test and fecal characteristics
 were used to evaluate the visceral sensitivity of rats. The expression of
 CD103, a surface molecule specific to dendritic cells in the colon tissue
 of the ileocecal region, was detected by immunohistochemistry. Flow
 separation technique was employed to isolate dendritic cells from
 mesenteric lymph nodes and CD4+T lymphocytes from spleen, and the
 dendritic cells were co-cultured with T lymphocytes in vitro. MTT assay
 was employed to detect the ability of dendritic cells to promote the
 proliferation of initial T lymphocytes. The cytokine levels of IL-12
 and INF-γ secreted through Th1-pathway, and IL-4 and IL-9
 secreted through Th2-pathway were determined by
 ELISA.Results: Compared with the blank control group and model
 control group, the number of CD103-labeled dendritic cells in the model
 group were significantly increased. Dendritic cells from intestinal lymph
 nodes developed enhanced ability to promote the proliferation of spleen
 CD4+Tcell, and secreted more IL-4 and IL-9 than that in the
 control group while the secretion of IL-12 was significantly reduced (P
 &lt; 0.05).
description: 1. AWR test were used to evaluate the
 visceral sensitivity of rats. The values mean balloon pressure. The
 statistical data was in the
 document pressure.sav
 2. The fecal characteristics were
 used to evaluate the visceral sensitivity of rats. The number of each
 stool characteristics were summoned in the
 stool.sav. 3. MTT assay was employed to
 detect the ability of dendritic cells to promote the proliferation of
 initial T lymphocytes. The variables were mean MTT values. The raw data
 was in the document
 MTT.sav 4. The cytokine levels of IL-12
 and INF-γ secreted through Th1-pathway, and IL-4 and IL-9 secreted through
 Th2-pathway were determined by ELISA. The variables were mean ELISA
 values. The statistical data was in the
 document IL-4IL-9.sav；IL-12IFN-γ.sav 5. The Y-axis means groups and the X-axis on behalf of the test items in each SAV.files  6. All the primary data were summarized in Excel.
description: The fecal characteristics were
 used to evaluate the visceral sensitivity of rats. The number of each
 stool characteristic were summoned in the stool.sav. The variables were
 mean the stool numbers in each fecal type. For
 example in model group the number of hard stool was 8；the number of soft
 number was 12. the number of loose stool was
 4.  All the primary data were
 summarized in the Excel file, can
 be re-used for
 necessary.