DOI: 10.5061/dryad.70rxwdc5w
title: Data from: Simultaneous determination of diosmin and hesperidin in combined pharmaceutical preparation by synchronous fluorescence spectrofluorimetric method

description: Diosmin (DSM) and hesperidin (HSP) binary mixture was analyzed
 simultaneously by developing a sensitive, rapid, and simple synchronous
 spectrofluorimetric method. In methanol at ∆λ of 100 nm, the relative
 synchronous fluorescence intensities (RSFI) of both medications were
 measured. It was shown that these intensities were influenced by distinct
 experimental factors. The optimization and detailed study of these
 parameters were conducted. For DSM and HSP, respectively, the plots of
 synchronous fluorescence intensity-concentration were found to be
 rectilinear across the ranges of 0.5-5.0 µg/mL and 0.2-3.0 µg/mL. For DSM
 and HSP, respectively, 0.107 µg/mL and 0.048 µg/mL were the detection
 limits and 0.323 and 0.144 µg/mL were the limits of quantification. The
 method described in this study was effectively employed to estimate the
 quantities of both drugs present in commercially available mixed tablets.
 The results produced using this method were then compared favorably to
 results obtained using a different method for comparison.
description: <strong>Description of Method used for
 collection/generation of data: </strong> A series
 of 10 mL volumetric flasks were filled with aliquots of the DSM and HSP
 standard solutions covering the working concentration range shown in Table
 1. Then, methanol was added to dilute them to the mark. By scanning both
 monochromators with 10 nm excitation and emission windows and a constant
 wavelength difference (Δλ) of 100 nm, SF spectra of the solutions were
 captured. For DSM and HSP, the SF spectra's intensities were
 calculated to be at 280 nm and 390 nm, respectively. The experiment was
 run in parallel with a blank one. To obtain the calibration curves,
 plotting of the RSFI with the final drug concentrations (µg/mL) was done.
 An alternative was to derive the corresponding regression
 equations.
description: # Data from: Simultaneous determination of diosmin and hesperidin in
 combined pharmaceutical preparation by synchronous fluorescence
 spectrofluorimetric method
 \[[https://doi.org/10.5061/dryad.70rxwdc5w](https://doi.org/10.5061/dryad.70rxwdc5w)] ## Description of the data and file structure **File List:** 1.Calibration_data_for_analysis_of_DSM_by_Synchronous_Fluorescence\_ Spectrofluorimetric_Method.xlsx A linear calibration curve was obtained when the RSFI was plotted against the final drug concentrations of DSM (µg/mL). 2.Calibration_data_for_analysis_of_HSP_by_Synchronous_Fluorescence\_ Spectrofluorimetric_Method.xlsx A linear calibration curve was obtained when the RSFI was plotted against the final drug concentrations of HSP (µg/mL). **Abbreviations:** RSFI is the relative synchronous fluorescence intensity. n is the number of samples. r is the regression coefficient. SD is the standard deviation. RSD is the relative standard deviation. DSM is the abbreviation of Diosmin drug. HSP is the abbreviation of Hesperidin drug. ## Sharing/Access information **1. Licenses/restrictions placed on the data:** No License needed for this data. **2. Links to publications that cite or use the data:**  * [https://doi.org/10.5061/dryad.70rxwdc5w](https://doi.org/10.5061/dryad.70rxwdc5w) **3. Was the data derived from another source?** No **4. Is this dataset included in our main manuscript?** No **5. Recommended citation for this dataset:** H. Askar, M.E. Fathy, M.M. Tolba, Fatma A. Aly, and Mohammed E.-S. Metwally (2024), Simultaneous Determination of Diosmin and Hesperidin in Combined Pharmaceutical Preparation by Synchronous Fluorescence Spectrofluorimetric Method. ## Code/Software **Instrument- or software-specific information needed to interpret the data:** Eclipse fluorescence spectrophotometer was used to record measurements and fluorescence spectra and Agilent Technologies provided the Xenon flash lamp for it. The slit width was 5 nm, and the high voltage mode was used (800 v). Smoothing factor 15 was used. At 280 nm and 390 nm for DSM and HSP, respectively, the SF spectra were estimated.