DOI: 10.5061/dryad.2598k
title: Data from: Effects of soil resources on expression of a sexual conflict over timing of stigma receptivity in a mixed-mating plant

description: While environmental factors strongly influence plant growth and
 reproduction, less is known about environmental effects on sexual
 selection and sexual conflict. In this study on mixed-mating Collinsia
 heterophylla we investigated whether soil resource environment affected
 traits associated with sexual conflict. In C. heterophylla a sexual
 conflict over timing of stigma receptivity occurs. Early stigma
 receptivity benefits pollen parents by securing paternity while late
 stigma receptivity benefits female fitness in terms of increased seed
 production. We performed hand-pollinations combining recipients and donors
 grown either in high or low resource environments and asked whether these
 treatments influenced sexual conflict traits – recipient- and donor-based
 influence on timing of stigma receptivity – and conflict costs related to
 reduced early seed production. We also asked whether resource environment
 affected nine traits related to general fitness and mating system. Sexual
 conflict-associated traits – timing of stigma receptivity and seed
 production – were generally unaffected by resource environment. While no
 universal effect of resources was detected, we did observe donor-specific
 responses to environment, suggesting that environment can nonetheless
 contribute to variation in timing of stigma receptivity. Recipients grown
 under low resources showed pronounced differences among donors for number
 of seeds per capsule, indicating that recipients favour some donors over
 others under resource-low conditions. Moreover, high resources increased
 number of flowers but reduced pollen germination rate, while other traits
 were unaffected, indicating variation in the response to resource
 environment for fitness- and mating system-traits. Our results suggest
 that even though soil resource environment had a low impact on the sexual
 conflict traits and related costs in C. heterophylla, it generated
 variability in pollen donor-influence on this trait and in recipient
 sorting among donors. Thus, it is possible that both sexual conflict and
 sexual selection is affected by environmental factors not only in animals
 but also in plants.
description: Data_stage first seed
 set_experiment 1Data on stage of first
 seed set following experimental crosses at floral developmental stage 1-4
 in Collinsia heterophylla. Recipients and pollen donor were grown in
 either high or low levels of soil resources.Data_seed
 traits_experiment 1Data on seed traits
 following experimental crosses at floral developmental stage 1-4 in
 Collinsia heterophylla. Recipients and pollen donor were grown in either
 high or low levels of soil resources. Seed traits = number of seeds per
 capsule, capsule seed weight, maturity time from cross till
 harvest.Data_plant
 traits_experiment 1Data on traits for
 plants grown in either high or low levels of soil resources in Collinsia
 heterophylla. Traits = flower start estimated as day from the first plant
 started flowering, plant height, number of branches, number of flowers on
 the main branch.Data_anther-stigma
 contact_experiment 1Data on anther-stigma
 contact estimated at floral developmental stage 1-4 of flowers for plants
 grown in either high or low levels of soil resources in Collinsia
 heterophylla. S = stigma shorter than anther, C = stigma-anther contact, L
 = stigma longer than antherData_plant
 height_experiment 2Data on plant height for
 plants grown in either high or low levels of soil resources in
 supplementary experiment in Collinsia heterophylla.Data_pollen
 traits_experiment 2Data on pollen traits
 assessed in vitro for plants grown in either high or low levels of soil
 resources in supplementary experiment in Collinsia heterophylla. Pollen
 traits = germination rate (germinated vs. ungerminated) after 15 min,
 germination rate (germinated vs. ungerminated) after 1h, 45 min, pollen
 tube growth rate estimated for ten replicate pollen tubes after 1h, 45
 min.