cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12210665.v1
title: Robotic endoscopy. A review of the literature

description: Abstract Purpose To present new endoscopic robotic devices in the context of minimally invasive procedures with high precision and automation. Methods Review of the literature by December 2018 on robotic endoscopy. Results We present the studies and investments for robotic implementation and flexible endoscopy evolution. We divided them into forceps manipulation platforms, active endoscopy and endoscopic capsule. They try to improve forceps handling and stability and to promote active movement. Conclusion The implementation and propagation of robotic models depend on doing what the endoscopist is unable to. The new devices are moving forward in this direction.
DOI: 10.6084/m9.figshare.12210704
title: Gene stacking as a strategy to confer characteristics of agronomic importance in plants by genetic engineering

description: ABSTRACT: Gene stacking refers to the introduction of two or more transgenes of agronomic interest in the same plant. The main methods for genetically engineering plants with gene stacking involve (i) the simultaneous introduction, by the co-transformation process, and (ii) the sequential introduction of genes using the re-transformation processes or the sexual crossing between separate transgenic events. In general, the choice of the best method varies according to the species of interest and the availability of genetic constructions and preexisting transgenic events. We also present here the use of minichromosome technology as a potential future gene stacking technology. The purpose of this review was to discuss aspects related to the methodology for gene stacking and trait stacking (a gene stacking strategy to combine characteristics of agronomical importance) by genetic engineering. In addition, we presented a list of crops and genes approved commercially that have been used in stacking strategies for combined characteristics and a discussion about the regulatory standards. An increased number of approved and released gene stacking events reached the market in the last decade. Initially, the most common combined characteristics were herbicide tolerance and insect resistance in soybean and maize. Recently, commercially available varieties were released combining these traits with drought tolerance in these commodities. New traits combinations are reaching the farmer’s fields, including higher quality, disease resistant and nutritional value improved. In other words, gene stacking is growing as a strategy to contribute to food safety and sustainability.
DOI: 10.6084/m9.figshare.12210761
title: Population and reproductive traits of a freshwater amphipod (Crustacea, Peracarida, Hyalellidae) from northwest of the state of Rio Grande do Sul, Brazil

description: Abstract The study of population and reproductive traits provides information about the ecological structure of natural populations. This study aimed to characterize dynamics and reproductive traits of Hyalella palmeirensis from a natural pond from southern Brazil. The amphipods were sampled monthly (August 2012 to July 2013) by a person with the aid of a hand net for 20 minutes. Ovigerous females and pre-copulatory pairs were individualized in the field. A total of 12,325 individuals were sampled, being 1,421 males, 6,983 females (including 215 ovigerous females) and 3,921 juveniles. Paired and unpaired males were significantly greater in size than females. There was a positive correlation between body size (CL) of paired males and females. Males and females showed bimodal distribution. Total sex ratio favored females, and these were more frequent in almost all months. Ovigerous females and precopulatory pairs were found throughout the year, but with high frequency in winter and autumn, respectively, characterizing a seasonal reproduction. Juveniles were sampled throughout the year, with greater intensity in the spring. The mean fecundity was 19.6 ± 4.34 eggs. No reduction in the number of eggs was observed during embryonic development. The results observed in H. palmeirensis demonstrate that this species has a population and reproductive dynamics very similar to other species of Hyalella already analyzed in southern Brazil. Moreover, it can be seen that although the H. palmeirensis occurs in an environment with anthropic influence (soy cultivation,) the population is managing to remain in the area, with reproduction and recruitment in most months of year.
DOI: 10.6084/m9.figshare.12210704.v1
title: Gene stacking as a strategy to confer characteristics of agronomic importance in plants by genetic engineering

description: ABSTRACT: Gene stacking refers to the introduction of two or more transgenes of agronomic interest in the same plant. The main methods for genetically engineering plants with gene stacking involve (i) the simultaneous introduction, by the co-transformation process, and (ii) the sequential introduction of genes using the re-transformation processes or the sexual crossing between separate transgenic events. In general, the choice of the best method varies according to the species of interest and the availability of genetic constructions and preexisting transgenic events. We also present here the use of minichromosome technology as a potential future gene stacking technology. The purpose of this review was to discuss aspects related to the methodology for gene stacking and trait stacking (a gene stacking strategy to combine characteristics of agronomical importance) by genetic engineering. In addition, we presented a list of crops and genes approved commercially that have been used in stacking strategies for combined characteristics and a discussion about the regulatory standards. An increased number of approved and released gene stacking events reached the market in the last decade. Initially, the most common combined characteristics were herbicide tolerance and insect resistance in soybean and maize. Recently, commercially available varieties were released combining these traits with drought tolerance in these commodities. New traits combinations are reaching the farmer’s fields, including higher quality, disease resistant and nutritional value improved. In other words, gene stacking is growing as a strategy to contribute to food safety and sustainability.
DOI: 10.6084/m9.figshare.12210761.v1
title: Population and reproductive traits of a freshwater amphipod (Crustacea, Peracarida, Hyalellidae) from northwest of the state of Rio Grande do Sul, Brazil

description: Abstract The study of population and reproductive traits provides information about the ecological structure of natural populations. This study aimed to characterize dynamics and reproductive traits of Hyalella palmeirensis from a natural pond from southern Brazil. The amphipods were sampled monthly (August 2012 to July 2013) by a person with the aid of a hand net for 20 minutes. Ovigerous females and pre-copulatory pairs were individualized in the field. A total of 12,325 individuals were sampled, being 1,421 males, 6,983 females (including 215 ovigerous females) and 3,921 juveniles. Paired and unpaired males were significantly greater in size than females. There was a positive correlation between body size (CL) of paired males and females. Males and females showed bimodal distribution. Total sex ratio favored females, and these were more frequent in almost all months. Ovigerous females and precopulatory pairs were found throughout the year, but with high frequency in winter and autumn, respectively, characterizing a seasonal reproduction. Juveniles were sampled throughout the year, with greater intensity in the spring. The mean fecundity was 19.6 ± 4.34 eggs. No reduction in the number of eggs was observed during embryonic development. The results observed in H. palmeirensis demonstrate that this species has a population and reproductive dynamics very similar to other species of Hyalella already analyzed in southern Brazil. Moreover, it can be seen that although the H. palmeirensis occurs in an environment with anthropic influence (soy cultivation,) the population is managing to remain in the area, with reproduction and recruitment in most months of year.
DOI: 10.6084/m9.figshare.12210821
title: Resin-dentin bond stability of etch-and-rinse adhesive systems with different concentrations of MMP inhibitor GM1489

description: Abstract Enzymatic degradation of the hybrid layer can be accelerated by the activation of dentin metalloproteinases (MMP) during the bonding procedure. MMP inhibitors may be used to contain this process. Objective To evaluate the degree of conversion (DC%), dentin bond strength (µTBS) (immediate and after 1 year of storage in water), and nanoleakage of an experimental (EXP) and a commercial (SB) adhesive system, containing different concentrations of the MMP inhibitor GM1489: 0, 1 µM, 5 µM and 10 µM. Methodology DC% was evaluated by FT-IR spectroscopy. Dentin bond strength was evaluated by µTBS test. Half of beams were submitted to the µTBS test after 24 h and the other half, after storage for 1 year. From each tooth and storage time, 2 beams were reserved for nanoleakage testing. Data were analyzed using ANOVA and Tukey’s test to compare means (α=0.05). Results All adhesive systems maintained the µTBS after 1 year of storage. Groups with higher concentrations of inhibitor (5 µM and 10 µM) showed higher µTBS values than groups without inhibitor or with 1 µM. The nanoleakage values of all groups showed no increase after 1 year of storage and values were similar for SB and EXP groups, in both storage periods. The inhibitor did not affect the DC% of the EXP groups, but the SB5 and SB10 groups showed higher DC% values than those of SB0 and SB1. Conclusions The incorporation of GM1489 in the adhesive systems had no detrimental effect on DC%. The concentrations of 5 µM GM1489 for SB and 5 µM or 10 µM for EXP provided higher μTBS than groups without GM1489, in the evaluation after 1 year of storage; whereas the concentration of inhibitor did not affect adhesive systems nanoleakage.
DOI: 10.6084/m9.figshare.12210821.v1
title: Resin-dentin bond stability of etch-and-rinse adhesive systems with different concentrations of MMP inhibitor GM1489

description: Abstract Enzymatic degradation of the hybrid layer can be accelerated by the activation of dentin metalloproteinases (MMP) during the bonding procedure. MMP inhibitors may be used to contain this process. Objective To evaluate the degree of conversion (DC%), dentin bond strength (µTBS) (immediate and after 1 year of storage in water), and nanoleakage of an experimental (EXP) and a commercial (SB) adhesive system, containing different concentrations of the MMP inhibitor GM1489: 0, 1 µM, 5 µM and 10 µM. Methodology DC% was evaluated by FT-IR spectroscopy. Dentin bond strength was evaluated by µTBS test. Half of beams were submitted to the µTBS test after 24 h and the other half, after storage for 1 year. From each tooth and storage time, 2 beams were reserved for nanoleakage testing. Data were analyzed using ANOVA and Tukey’s test to compare means (α=0.05). Results All adhesive systems maintained the µTBS after 1 year of storage. Groups with higher concentrations of inhibitor (5 µM and 10 µM) showed higher µTBS values than groups without inhibitor or with 1 µM. The nanoleakage values of all groups showed no increase after 1 year of storage and values were similar for SB and EXP groups, in both storage periods. The inhibitor did not affect the DC% of the EXP groups, but the SB5 and SB10 groups showed higher DC% values than those of SB0 and SB1. Conclusions The incorporation of GM1489 in the adhesive systems had no detrimental effect on DC%. The concentrations of 5 µM GM1489 for SB and 5 µM or 10 µM for EXP provided higher μTBS than groups without GM1489, in the evaluation after 1 year of storage; whereas the concentration of inhibitor did not affect adhesive systems nanoleakage.
DOI: 10.6084/m9.figshare.12210503
title: Combined use of mitochondrial and nuclear genetic markers further reveal immature marine turtle hybrids along the South Western Atlantic

description: Abstract Marine turtle hybridization is usually sporadic and involves reports of only a few individuals; however, Brazilian populations have high hybridization rates. Here we investigated the presence of hybrids in morphologically identified immature hawksbills (Eretmochelys imbricata) along the South Western Atlantic (SWA). We sequenced one mitochondrial (D-Loop) and three nuclear DNA (RAG1, RAG2, and CMOS) markers to better understand the patterns and characteristics of hybrids. We identified 22 hybrids (n = 270), 11 of them at the extreme South of the SWA. Uruguay had the highest hybrid frequency in the SWA (~37.5%) followed by southern Brazil with 30%. These are common areas for loggerheads (Caretta caretta) but uncommon for hawksbills, and these hybrids may be adopting the behavior of loggerheads. By analyzing nuclear markers, we can infer that 50% of the sampled hybrids are first generation (F1) and 36% are the result of backcrosses between hybrids and pure E. imbricata (&gt; F1). We also report for the first time immature E. imbricata x Lepidochelys olivacea hybrids at the Brazilian coast. Considering the high frequency of hybrids in the SWA, continuous monitoring should be performed to assess the fitness, genetic integrity, and extent of changes in the gene pools of involved populations.
DOI: 10.6084/m9.figshare.12210413
title: LATIN AMERICA IN THE 21ST CENTURY AND PRODUCTIVE RESTRUCTURING IN THE LIGHT OF THE ECLAC THEORY

description: ABSTRACT Throughout the 70 years of ECLAC's existence, the thinking that inspired its main works have been based on the structural historical method and the center-periphery model, according to which the movements of the world’s economy would determine the development of the "peripheral" regions. In the 1990s, the countries in Latin America adhered to the structural adjustment models, bringing about the triumph of neoliberalism. As the economies in the region experienced mediocre industrial performance in the following decade but benefited from the favorable international economic environment of the 2000s, with the expansion of commodity sectors, the debate on the need for regional industrialization in the region is still alive. Thus, this article aims to analyze the structural change in Latin America economies at the beginning of the 21st century, based on ECLAC's contributions. The analysis suggests that the restructuring of Latin American economies has happened in a much complex way and that the understanding of such a process involves the analysis of many variables embodied in ECLAC's vision of development.
DOI: 10.6084/m9.figshare.12210503.v1
title: Combined use of mitochondrial and nuclear genetic markers further reveal immature marine turtle hybrids along the South Western Atlantic

description: Abstract Marine turtle hybridization is usually sporadic and involves reports of only a few individuals; however, Brazilian populations have high hybridization rates. Here we investigated the presence of hybrids in morphologically identified immature hawksbills (Eretmochelys imbricata) along the South Western Atlantic (SWA). We sequenced one mitochondrial (D-Loop) and three nuclear DNA (RAG1, RAG2, and CMOS) markers to better understand the patterns and characteristics of hybrids. We identified 22 hybrids (n = 270), 11 of them at the extreme South of the SWA. Uruguay had the highest hybrid frequency in the SWA (~37.5%) followed by southern Brazil with 30%. These are common areas for loggerheads (Caretta caretta) but uncommon for hawksbills, and these hybrids may be adopting the behavior of loggerheads. By analyzing nuclear markers, we can infer that 50% of the sampled hybrids are first generation (F1) and 36% are the result of backcrosses between hybrids and pure E. imbricata (&gt; F1). We also report for the first time immature E. imbricata x Lepidochelys olivacea hybrids at the Brazilian coast. Considering the high frequency of hybrids in the SWA, continuous monitoring should be performed to assess the fitness, genetic integrity, and extent of changes in the gene pools of involved populations.
DOI: 10.6084/m9.figshare.12210413.v1
title: LATIN AMERICA IN THE 21ST CENTURY AND PRODUCTIVE RESTRUCTURING IN THE LIGHT OF THE ECLAC THEORY

description: ABSTRACT Throughout the 70 years of ECLAC's existence, the thinking that inspired its main works have been based on the structural historical method and the center-periphery model, according to which the movements of the world’s economy would determine the development of the "peripheral" regions. In the 1990s, the countries in Latin America adhered to the structural adjustment models, bringing about the triumph of neoliberalism. As the economies in the region experienced mediocre industrial performance in the following decade but benefited from the favorable international economic environment of the 2000s, with the expansion of commodity sectors, the debate on the need for regional industrialization in the region is still alive. Thus, this article aims to analyze the structural change in Latin America economies at the beginning of the 21st century, based on ECLAC's contributions. The analysis suggests that the restructuring of Latin American economies has happened in a much complex way and that the understanding of such a process involves the analysis of many variables embodied in ECLAC's vision of development.
DOI: 10.6084/m9.figshare.12210662
title: Spatial distribution of specialized cardiac care units in the state of Santa Catarina

description: OBJECTIVE To analyze the methodology used for assessing the spatial distribution of specialized cardiac care units. METHODS A modeling and simulation method was adopted for the practical application of cardiac care service in the state of Santa Catarina, Southern Brazil, using the p-median model. As the state is divided into 21 health care regions, a methodology which suggests an arrangement of eight intermediate cardiac care units was analyzed, comparing the results obtained using data from 1996 and 2012. RESULTS Results obtained using data from 2012 indicated significant changes in the state, particularly in relation to the increased population density in the coastal regions. The current study provided a satisfactory response, indicated by the homogeneity of the results regarding the location of the intermediate cardiac care units and their respective regional administrations, thereby decreasing the average distance traveled by users to health care units, located in higher population density areas. The validity of the model was corroborated through the analysis of the allocation of the median vertices proposed in 1996 and 2012. CONCLUSIONS The current spatial distribution of specialized cardiac care units is more homogeneous and reflects the demographic changes that have occurred in the state over the last 17 years. The comparison between the two simulations and the current configuration showed the validity of the proposed model as an aid in decision making for system expansion.
DOI: 10.6084/m9.figshare.12210362
title: Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction

description: ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).
DOI: 10.6084/m9.figshare.12210662.v1
title: Spatial distribution of specialized cardiac care units in the state of Santa Catarina

description: OBJECTIVE To analyze the methodology used for assessing the spatial distribution of specialized cardiac care units. METHODS A modeling and simulation method was adopted for the practical application of cardiac care service in the state of Santa Catarina, Southern Brazil, using the p-median model. As the state is divided into 21 health care regions, a methodology which suggests an arrangement of eight intermediate cardiac care units was analyzed, comparing the results obtained using data from 1996 and 2012. RESULTS Results obtained using data from 2012 indicated significant changes in the state, particularly in relation to the increased population density in the coastal regions. The current study provided a satisfactory response, indicated by the homogeneity of the results regarding the location of the intermediate cardiac care units and their respective regional administrations, thereby decreasing the average distance traveled by users to health care units, located in higher population density areas. The validity of the model was corroborated through the analysis of the allocation of the median vertices proposed in 1996 and 2012. CONCLUSIONS The current spatial distribution of specialized cardiac care units is more homogeneous and reflects the demographic changes that have occurred in the state over the last 17 years. The comparison between the two simulations and the current configuration showed the validity of the proposed model as an aid in decision making for system expansion.
DOI: 10.6084/m9.figshare.11609790
title: Comparison of cerebrospinal fluid lactate with physical, cytological, and other biochemical characteristics as prognostic factors in acute bacterial meningitis

description: ABSTRACT Bacterial meningitis (BM) is associated with a high morbidity and mortality. Cerebrospinal fluid (CSF) lactate may be used as a prognostic marker of this condition. We hypothesized that CSF lactate levels would remain elevated in participants who died of acute BM compared with those who recovered from this disease. Objective: To evaluate the potential use of lactate and other CSF biomarkers as prognostic markers of acute BM outcome. Methods: This retrospective, longitudinal study evaluated dynamic CSF biomarkers in 223 CSF samples from 49 patients who fulfilled the inclusion criteria of acute BM, with bacteria identified by CSF culturing. The participants were grouped according to outcome: death (n = 9; 18.37%) and survival (n = 40; 81.63%). All participants received appropriate antibiotic treatment. Results: In the logistic regression model, lactate concentration in the final CSF sample, xanthochromia, and CSF glucose variation between the first and last CSF samples were predictors of a poor outcome (death). In contrast, decrease in CSF white blood cell count and CSF percentage of neutrophils, increase in the percentage of lymphocytes, and normalization of the CSF lactate concentration in the last CSF sample were predictors of a good prognosis. Conclusion: The study confirmed the initial hypothesis. The longitudinal analysis of CSF lactate is an important predictor of prognosis in acute BM.