DOI: 10.5061/dryad.18931zd5w
title: Vaccinium virgatum (Ericaceae), a species to be recognized

description: Since its original publication in 1789, Vaccinium virgatum has been
 treated by most authors as an accepted species in V. sect. Cyanococcus. In
 the latest comprehensive taxonomic treatment of the section, however, it
 is treated as a synonym of the broadly circumscribed species V.
 corymbosum. Here we use morphology and estimation of ploidy level with
 flow cytometry in combination with previously published phylogenomic
 analysis based on high-throughput DNA sequencing to support the taxonomic
 status of V. virgatum as a species to be recognized. As circumscribed
 here, V. virgatum occurs in the southeastern U.S. Coastal Plain from
 Arkansas and Texas to northeastern Florida and South Carolina. An updated
 taxonomic treatment of the species, including an expanded description,
 distribution map by county, and a representative list of specimens
 examined by county is included. We provide a means of distinguishing V.
 virgatum from V. ashei, a similar species recently also segregated from V.
 corymbosum, and from presumed rabbiteye blueberry escapes from
 cultivation, which can occur both within and outside of the native range
 of V. virgatum. We designate a neotype for V. virgatum and lectotypes for
 V. virgatum vars. angustifolium, parvifolium, and speciosum.
description: The Angiosperms353 v1 target capture kit (Johnson et al., 2019)
 was used for targeted enrichment of each sample.Raw sequences were
 filtered and processed with the Trim Galore wrapper script (v.0.6.5) to
 trim adapters and low-quality reads based on a given Phred quality score
 cutoff (-q 20). Consensus read assembly for target loci was performed with
 the default settings in HybPiper v2. Supercontig sequences (introns +
 exons) were assembled in HybPiper. Following alignment of individual loci
 with MAFFT, columns with &gt;50% missing data were removed, and
 concatenated. Vaccinium macrocarpon ("V_macrocarpon_CY234")
 serves as the outgroup sample.
description: # Vaccinium virgatum (Ericaceae), a species to be recognized
 [https://doi.org/10.5061/dryad.18931zd5w](https://doi.org/10.5061/dryad.18931zd5w) ## Description of the data and file structure The Angiosperms353 v1 target capture kit (Johnson et al., 2019) was used for targeted enrichment of each sample. Raw sequences were filtered and processed with the Trim Galore wrapper script (v.0.6.5) to trim adapters and low-quality reads based on a given Phred quality score cutoff (-q 20). Consensus read assembly for target loci was performed with the default settings in HybPiper v2. Supercontig sequences (introns + exons) were assembled in HybPiper. Following alignment of individual loci with MAFFT, columns with &gt;50% missing data were removed, and concatenated. Vaccinium macrocarpon ("V_macrocarpon_CY234") serves as the outgroup sample. ### Files and variables #### File: 00\_concat\_supercontigs\_50missing\_2x\_4x\_6x\_v3.partitions.txt **Description:** corresponding partition file for 352 'supercontig' loci #### File: 00\_concat\_supercontigs\_50missing\_2x\_4x\_6x\_v3.fa **Description:** concatenated IUPAC alignment file in .fasta format