cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21353313
title: Masseter muscle flap for reconstruction of intra-oral defects in patients with early cancer of posterior-inferior parts of the oral cavity

description: Abstract Introduction Early carcinomas of the oral cavity in the posterior-inferior regions poses a challenge for reconstruction due to the lack of muscle support underneath and the limited space available to use some of the frequently-used flaps. Objective This study was done to evaluate the efficacy of the superiorly based masseter muscle flap in reconstruction of intra-oral post- ablation defects in patients with early oral carcinoma of the posterior-inferior part of the oral cavity. Methods A superiorly based masseter muscle flap were used to reconstruct the post-surgical intra- oral defect in 60 patients with early squamous cell carcinoma (T &lt; 4 cm) of the posterior-inferior part of the oral cavity. The patients were followed up at 1-week and 1-month postoperatively to check for flap viability, complications, change in mouth opening and deviation of the mandible on mouth opening. To rule out any recurrence in the oral cavity masseter flaps, the patients were followed up for 1 year. Results The flap was viable in all patients and underwent mucosalization. 7/60 patients had postoperative infections, while 2/60 patients developed an oro-cutaneous fistula which required a secondary corrective procedure. The mean ± standard deviation of change in mouth opening at 1 week postoperatively was +1.917 ± 3.36 mm, which increased to +2.633 ± 2.95 mm at 1 month after surgery. The Friedman test revealed that there was a statistically significant change in mouth opening from preoperative period to the1 week and 1 month postoperative periods (p = 0.000). Female patients showed better improvement in mouth opening postoperatively. The ipsilateral deviation of the mandible on mouth opening was between 0-5 mm in 39 patients, 5-10 mm in 17 patients and more than 10 mm in 4 patients. There were no recurrences noted in the masseter flaps used. Conclusion The study infers that the superiorly based masseter muscle flap is a reliable method for reconstruction in early oral cancer patients yielding good functional results and acceptable cosmesis with nominal postoperative complications.
DOI: 10.6084/m9.figshare.21353313.v1
title: Masseter muscle flap for reconstruction of intra-oral defects in patients with early cancer of posterior-inferior parts of the oral cavity

description: Abstract Introduction Early carcinomas of the oral cavity in the posterior-inferior regions poses a challenge for reconstruction due to the lack of muscle support underneath and the limited space available to use some of the frequently-used flaps. Objective This study was done to evaluate the efficacy of the superiorly based masseter muscle flap in reconstruction of intra-oral post- ablation defects in patients with early oral carcinoma of the posterior-inferior part of the oral cavity. Methods A superiorly based masseter muscle flap were used to reconstruct the post-surgical intra- oral defect in 60 patients with early squamous cell carcinoma (T &lt; 4 cm) of the posterior-inferior part of the oral cavity. The patients were followed up at 1-week and 1-month postoperatively to check for flap viability, complications, change in mouth opening and deviation of the mandible on mouth opening. To rule out any recurrence in the oral cavity masseter flaps, the patients were followed up for 1 year. Results The flap was viable in all patients and underwent mucosalization. 7/60 patients had postoperative infections, while 2/60 patients developed an oro-cutaneous fistula which required a secondary corrective procedure. The mean ± standard deviation of change in mouth opening at 1 week postoperatively was +1.917 ± 3.36 mm, which increased to +2.633 ± 2.95 mm at 1 month after surgery. The Friedman test revealed that there was a statistically significant change in mouth opening from preoperative period to the1 week and 1 month postoperative periods (p = 0.000). Female patients showed better improvement in mouth opening postoperatively. The ipsilateral deviation of the mandible on mouth opening was between 0-5 mm in 39 patients, 5-10 mm in 17 patients and more than 10 mm in 4 patients. There were no recurrences noted in the masseter flaps used. Conclusion The study infers that the superiorly based masseter muscle flap is a reliable method for reconstruction in early oral cancer patients yielding good functional results and acceptable cosmesis with nominal postoperative complications.
DOI: 10.6084/m9.figshare.21353322
title: Management and reclamation of salt-affected soils: general assessment and experiences in the Brazilian semiarid region

description: ABSTRACT Salt-affected soils occupy extensive areas in arid and semi-arid regions around the world, and impact food production, particularly when salinization processes occur in irrigated areas. In this context, the possibility of using techniques for the reclamation of these soils needs to be considered. However, many of these techniques are inaccessible to most farmers in the Brazilian semi-arid region, given the high costs, mainly when it is necessary to install a subsurface drainage system. Thus, there is a need to evaluate other soil reclamation strategies as well as the possibility of using these areas for other purposes. This review covers topics related to the characterization and impacts of salt-affected soils, the influence of soil microorganisms, and strategies for soil reclamation, such as the use of chemical and organic conditioners, bio-drainage, phytoremediation, and revegetation with tolerant species. The topics addressed in this review indicate new possibilities for the reclamation and management of salt-affected soils in the Brazilian semi-arid region. A case study is also presented, which demonstrates that the use of salt-affected soils is defined by technical, social, and economic aspects.
DOI: 10.6084/m9.figshare.21353322.v1
title: Management and reclamation of salt-affected soils: general assessment and experiences in the Brazilian semiarid region

description: ABSTRACT Salt-affected soils occupy extensive areas in arid and semi-arid regions around the world, and impact food production, particularly when salinization processes occur in irrigated areas. In this context, the possibility of using techniques for the reclamation of these soils needs to be considered. However, many of these techniques are inaccessible to most farmers in the Brazilian semi-arid region, given the high costs, mainly when it is necessary to install a subsurface drainage system. Thus, there is a need to evaluate other soil reclamation strategies as well as the possibility of using these areas for other purposes. This review covers topics related to the characterization and impacts of salt-affected soils, the influence of soil microorganisms, and strategies for soil reclamation, such as the use of chemical and organic conditioners, bio-drainage, phytoremediation, and revegetation with tolerant species. The topics addressed in this review indicate new possibilities for the reclamation and management of salt-affected soils in the Brazilian semi-arid region. A case study is also presented, which demonstrates that the use of salt-affected soils is defined by technical, social, and economic aspects.
DOI: 10.6084/m9.figshare.21353327
title: Searching for mechanisms behind meiotic abnormalities in Crotalaria spectabilis Roth

description: ABSTRACT The identification of epigenetic marks associated with problems in the meiotic process can enlighten the mechanisms underlying the irregularities and the impacts in the genetic constitution of gametes. Therefore, this study aimed to verify the relationship between the pattern of phosphorylation in serine 10 of histone H3 (H3S10ph), a (peri) centromeric epigenetic mark, with meiotic abnormalities in a wild population of Crotalaria spectabilis Roth. The main abnormalities observed were transfer of genetic material through cytoplasmatic connections, DNA elimination and abnormal spindle array. Different forms of elimination (chromatin fragmentation, ring formation, lagging chromosomes and micronuclei) were observed from the early phases until tetrad formation. The eliminated chromatin was either positive or negative for the immunosignal of H3S10ph, so it may be occurring elimination of acentric fragments, as well as of chromosomes with active or inactive centromeres. Therefore, dysfunctional centromere is not the only candidate cause for elimination. The transfer of genetic material and the abnormal spindle arrays are evidence that this population can produce aneuploid gametes and 2n pollen grains.
DOI: 10.6084/m9.figshare.21353327.v1
title: Searching for mechanisms behind meiotic abnormalities in Crotalaria spectabilis Roth

description: ABSTRACT The identification of epigenetic marks associated with problems in the meiotic process can enlighten the mechanisms underlying the irregularities and the impacts in the genetic constitution of gametes. Therefore, this study aimed to verify the relationship between the pattern of phosphorylation in serine 10 of histone H3 (H3S10ph), a (peri) centromeric epigenetic mark, with meiotic abnormalities in a wild population of Crotalaria spectabilis Roth. The main abnormalities observed were transfer of genetic material through cytoplasmatic connections, DNA elimination and abnormal spindle array. Different forms of elimination (chromatin fragmentation, ring formation, lagging chromosomes and micronuclei) were observed from the early phases until tetrad formation. The eliminated chromatin was either positive or negative for the immunosignal of H3S10ph, so it may be occurring elimination of acentric fragments, as well as of chromosomes with active or inactive centromeres. Therefore, dysfunctional centromere is not the only candidate cause for elimination. The transfer of genetic material and the abnormal spindle arrays are evidence that this population can produce aneuploid gametes and 2n pollen grains.
DOI: 10.6084/m9.figshare.21353331
title: Ocular echobiometry and relationship with cranial and body morphometric parameters in Shih Tzu dogs

description: ABSTRACT The purpose of this paper is to determine the ocular echobiometry and investigate its correlation with cranial and body morphometric parameters in 50 adult Shih Tzu dogs. The echobiometric measurements of the anterior chamber (AC), vitreous chamber (VC), lens axial thickness (LTA), transverse lens thickness (LTT), and axial length of the eyeball (ALE) were obtained by two-dimensional ultrasonography. Morphometric measurements of bizygomatic distance (BDIST), frontal-occipital distance (FOD), withers height (WH), thoracic circumference (TC), and body length (BL) were also obtained. The mean of the AC depth was 2.83±0.50mm, the VC was 9.18±0.54mm, the LTA was 6.42±0.32mm and the LTT was 9.17±1.18mm, while the mean of the ALE was 18.82±0.66mm. There was no correlation between ocular echobiometric variables and cranial and body morphometric variables in adult dogs of the Shih Tzu breed, as well as no significant difference of these variables when considering gender and age of the dogs (p ≥ 0.05).
DOI: 10.6084/m9.figshare.21353331.v1
title: Ocular echobiometry and relationship with cranial and body morphometric parameters in Shih Tzu dogs

description: ABSTRACT The purpose of this paper is to determine the ocular echobiometry and investigate its correlation with cranial and body morphometric parameters in 50 adult Shih Tzu dogs. The echobiometric measurements of the anterior chamber (AC), vitreous chamber (VC), lens axial thickness (LTA), transverse lens thickness (LTT), and axial length of the eyeball (ALE) were obtained by two-dimensional ultrasonography. Morphometric measurements of bizygomatic distance (BDIST), frontal-occipital distance (FOD), withers height (WH), thoracic circumference (TC), and body length (BL) were also obtained. The mean of the AC depth was 2.83±0.50mm, the VC was 9.18±0.54mm, the LTA was 6.42±0.32mm and the LTT was 9.17±1.18mm, while the mean of the ALE was 18.82±0.66mm. There was no correlation between ocular echobiometric variables and cranial and body morphometric variables in adult dogs of the Shih Tzu breed, as well as no significant difference of these variables when considering gender and age of the dogs (p ≥ 0.05).
DOI: 10.6084/m9.figshare.21353334
title: Cross-cultural adaptation of the University Student Depression Inventory for Brazil

description: ABSTRACT Objectives: to adapt the University Student Depression Inventory for Brazil. Methods: we used 6 methodological stages: initial translation, translation synthesis, back-translation, expert committee, pre-final version testing and document submission to the scale authors for assessing the adaptation process. The judges were 2 methodologists, 1 health professional and 2 translators. For the pre-test, 30 undergraduate and graduate students participated. Results: the data were analyzed by Excel®, resulting in satisfactory content validity, a scale composed of 30 items, 3 domains and a total CVC of 0.91. Language clarity and practical relevance had a CVC of 0.91 and theoretical relevance of 0.90. Conclusions: the USDI-BR was cross-culturally adapted for Brazil, showing evidence of satisfactory content validity. After an analysis of reliability and convergent validity to be tested in future studies, multidisciplinary teams will be able to apply this scale to Brazilian students.
DOI: 10.6084/m9.figshare.21353334.v1
title: Cross-cultural adaptation of the University Student Depression Inventory for Brazil

description: ABSTRACT Objectives: to adapt the University Student Depression Inventory for Brazil. Methods: we used 6 methodological stages: initial translation, translation synthesis, back-translation, expert committee, pre-final version testing and document submission to the scale authors for assessing the adaptation process. The judges were 2 methodologists, 1 health professional and 2 translators. For the pre-test, 30 undergraduate and graduate students participated. Results: the data were analyzed by Excel®, resulting in satisfactory content validity, a scale composed of 30 items, 3 domains and a total CVC of 0.91. Language clarity and practical relevance had a CVC of 0.91 and theoretical relevance of 0.90. Conclusions: the USDI-BR was cross-culturally adapted for Brazil, showing evidence of satisfactory content validity. After an analysis of reliability and convergent validity to be tested in future studies, multidisciplinary teams will be able to apply this scale to Brazilian students.
DOI: 10.6084/m9.figshare.21353336
title: Investigation of Chlamydia sp., Morbillivirus sp., Parvovirus sp., Leishmania sp. and Alphacoronavirus sp. in captive giant anteaters (Myrmecophaga tridactyla)

description: ABSTRACT This research aimed to investigate the occurrence of Chlamydia sp., Morbillivirus sp., Parvovirus sp., Leishmania sp. and Alphacoronavirus sp. in captive giant anteaters. Blood and fecal samples were taken from 16 animals in institutions from the states of Minas Gerais, Bahia and Distrito Federal, which had been in captivity for at least a year. A commercial rapid chromatographic immunoassay test was used for detecting coronavirus and parvovirus antigens, in addition to antibodies against leishmaniasis, all results being negative. In the case of the test for antibodies against distemper, four (4/16; 25%) anteaters had an average titration, two (2/16; 12.5%) a low titration and ten (10/16; 62.5%) were non-reactive. Using the DOT-ELISA (dot blotting) method for detection of immunoglobulin G, only one specimen obtained a 1 : 40 titration. For the polymerase chain reaction tests for Leishmania and Chlamydia, all samples were negative.
DOI: 10.6084/m9.figshare.21353336.v1
title: Investigation of Chlamydia sp., Morbillivirus sp., Parvovirus sp., Leishmania sp. and Alphacoronavirus sp. in captive giant anteaters (Myrmecophaga tridactyla)

description: ABSTRACT This research aimed to investigate the occurrence of Chlamydia sp., Morbillivirus sp., Parvovirus sp., Leishmania sp. and Alphacoronavirus sp. in captive giant anteaters. Blood and fecal samples were taken from 16 animals in institutions from the states of Minas Gerais, Bahia and Distrito Federal, which had been in captivity for at least a year. A commercial rapid chromatographic immunoassay test was used for detecting coronavirus and parvovirus antigens, in addition to antibodies against leishmaniasis, all results being negative. In the case of the test for antibodies against distemper, four (4/16; 25%) anteaters had an average titration, two (2/16; 12.5%) a low titration and ten (10/16; 62.5%) were non-reactive. Using the DOT-ELISA (dot blotting) method for detection of immunoglobulin G, only one specimen obtained a 1 : 40 titration. For the polymerase chain reaction tests for Leishmania and Chlamydia, all samples were negative.
DOI: 10.6084/m9.figshare.21353337
title: Nutritional and metabolic parameters of sheep on deferred pasture with different strategy changes during the dry season

description: ABSTRACT To verify if strategies to reduce the height of Marandu grass pasture at beginning of the deferral period change intake and metabolic parameters in sheep during the dry season, 18 crossbred females, ½ Dorper + ½ Santa Inês were used, distributed in 9 Marandu grass paddock handles to 3 drawdown strategies at the beginning of deferral period: 1) maintenance of grass with 15cm for 5 months before deferral start (15/15cm); 2) maintenance of marandu grass with 25 cm for 5 months, but at the beginning of deferral it was reduced to 15cm (25/15cm); 3) maintenance of 35 cm grass for 5 months, but at the beginning of deferral it was reduced to 15cm (35/15cm). The experiment was conducted in a completely randomized design (CRD), with 6 repetitions and repeated measures over time. Strategies to reduce the height of Marandu grass pasture, at the beginning of the deferral period, do not change nutrient intake and energy and protein metabolism. However, changes occurring in the pasture during the dry season alter these variables, and deferral is an interesting strategy to be used for forage production in winter, however, to optimize its use, it is necessary to use supplementation with concentrated rich in fermentable carbohydrates.
DOI: 10.6084/m9.figshare.21353337.v1
title: Nutritional and metabolic parameters of sheep on deferred pasture with different strategy changes during the dry season

description: ABSTRACT To verify if strategies to reduce the height of Marandu grass pasture at beginning of the deferral period change intake and metabolic parameters in sheep during the dry season, 18 crossbred females, ½ Dorper + ½ Santa Inês were used, distributed in 9 Marandu grass paddock handles to 3 drawdown strategies at the beginning of deferral period: 1) maintenance of grass with 15cm for 5 months before deferral start (15/15cm); 2) maintenance of marandu grass with 25 cm for 5 months, but at the beginning of deferral it was reduced to 15cm (25/15cm); 3) maintenance of 35 cm grass for 5 months, but at the beginning of deferral it was reduced to 15cm (35/15cm). The experiment was conducted in a completely randomized design (CRD), with 6 repetitions and repeated measures over time. Strategies to reduce the height of Marandu grass pasture, at the beginning of the deferral period, do not change nutrient intake and energy and protein metabolism. However, changes occurring in the pasture during the dry season alter these variables, and deferral is an interesting strategy to be used for forage production in winter, however, to optimize its use, it is necessary to use supplementation with concentrated rich in fermentable carbohydrates.
DOI: 10.6084/m9.figshare.21353339
title: Soybean flour as a substrate to obtain Enterococcus durans bacteriocins

description: ABSTRACT Natural preservatives, such as enterocins, have been the focus of several studies for use in the food industry. However, the commercial media used to obtain enterocins are still expensive, presenting a disadvantage for large-scale production. In this study was developed four formulations of culture media containing soybean flour for obtaining Enterococcus durans enterocins. The antilisterial activity of E. durans MF5 enterocins obtained in soybean and MRS media (control) was characterized, with Listeria monocytogenes CLIP2032 and L. innocua CLIP12612 as the bacterial strains. The growth of E. durans (CFU/mL) was significantly affected by the incubation time in the soybean and MRS media (p &lt;0.05), but the composition of the media did not affect the cell development of the enterocin-producing microorganism. When evaluating the genes encoding enterocin synthesis, positive results were obtained for enterocin A, B, P, and X. When treated with proteolytic enzymes (α-chymotrypsin, protease, and proteinase-K), enterocin was inactivated, confirming its protein character. The antilisterial activity of the enterocins obtained in soybean media was up to 6,400 AU mL-1. Enterocins from soybean media M1 and M2 showed antibacterial activity at a concentration of 1 mg/mL after 6 h incubation. Thus, we show that culture media formulated with soybean flour are promising substrates for enterocin production that would allow the protocol to be expanded on a large scale.
DOI: 10.6084/m9.figshare.21353339.v1
title: Soybean flour as a substrate to obtain Enterococcus durans bacteriocins

description: ABSTRACT Natural preservatives, such as enterocins, have been the focus of several studies for use in the food industry. However, the commercial media used to obtain enterocins are still expensive, presenting a disadvantage for large-scale production. In this study was developed four formulations of culture media containing soybean flour for obtaining Enterococcus durans enterocins. The antilisterial activity of E. durans MF5 enterocins obtained in soybean and MRS media (control) was characterized, with Listeria monocytogenes CLIP2032 and L. innocua CLIP12612 as the bacterial strains. The growth of E. durans (CFU/mL) was significantly affected by the incubation time in the soybean and MRS media (p &lt;0.05), but the composition of the media did not affect the cell development of the enterocin-producing microorganism. When evaluating the genes encoding enterocin synthesis, positive results were obtained for enterocin A, B, P, and X. When treated with proteolytic enzymes (α-chymotrypsin, protease, and proteinase-K), enterocin was inactivated, confirming its protein character. The antilisterial activity of the enterocins obtained in soybean media was up to 6,400 AU mL-1. Enterocins from soybean media M1 and M2 showed antibacterial activity at a concentration of 1 mg/mL after 6 h incubation. Thus, we show that culture media formulated with soybean flour are promising substrates for enterocin production that would allow the protocol to be expanded on a large scale.