DOI: 10.22002/bswbr-46r90
title: Wild type NA1000

description: This is the cell shown in Fig. 2(G-G3), Li et al. 2007.
description: <strong>Tilt Series Date:</strong> 2006-05-11</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, Amira</p>
<strong>Collaborators and Roles:</strong> Cells cultured by Zhuo Li. Data collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle.  </p>
<strong>Sample Preparation:</strong> 4 ul of cell culture at different time points were mixed with 10nm colloidal gold and plunge-frozen onto glow-discharged Quantifoil R2/2, R2/1, or R1.2/1.3 grids using a Vitrobot (FEI).</p>
  wt105minpyeMay110634k180e12u019300.jpg: low mag lateamira.jpg: Segmentation lateamira1.jpg: Segmentation six.jpg: Segmentation
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-11-4/rawdata/</p>wt105minpyeMay110634k180e12u01_orig.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-11-4/rawdata/wt105minpyeMay110634k180e12u01_orig.mrc"
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        wt105minpyeMay110634k180e12u01.rec.mrc,  Reconstruction (Pixel Size 1.34 nm), 111.8 MB  
        
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        dwt105minpyeMay110634k180e12u01.rec_0015.mrc,  Reconstruction (Pixel Size 0.0 nm) Denoised tomogram, 111.8 MB  
        
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        dwt105minpyeMay110634k180e12u01.am,  Segmentation, 3.0 MB  
        
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        dwt105minpyeMay110634k180e12u01IM.am,  Segmentation, 2.2 MB  
        
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        dwt105minpyeMay110634k180e12u01_isosurfaceShow.am,  Segmentation, 2.6 MB  
        
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        dwt105minpyeMay110634k180e12u01OM_label.am,  Segmentation, 2.4 MB  
        
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        dwt105minpyeMay110634k180e12u01_vortex.am,  Segmentation, 3.1 MB  
        
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        dwt105minpyeMay110634k180e12u01_vortex1.am,  Segmentation, 1.8 MB  
        
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        FL_vortex.am,  Segmentation, 111.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-11-4/file_1361/FL_vortex.am"
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        lateFL.am,  Segmentation, 1.8 MB  
        
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        lateFL.surf,  Segmentation, 325.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-11-4/file_1365/lateFL.surf"
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        lateIM.surf,  Segmentation, 322.0 kB  
        
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        lateOM.surf,  Segmentation, 321.8 kB  
        
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