DOI: 10.5061/dryad.02v6wwq92
title: Cell size-dependent species sensitivity to nanoparticles underlies changes in phytoplankton diversity and productivity

description: Nanoparticle pollution has been shown to affect various organisms.
 However, the effects of nanoparticles on species interactions, and the
 role of species traits, such as body size, in modulating these effects,
 are not well understood. We addressed this issue using competing
 freshwater phytoplankton species exposed to copper oxide nanoparticles.
 Increasing nanoparticle concentration resulted in decreased phytoplankton
 species growth rates and community productivity (both abundance and
 biomass). Importantly, we consistently found that nanoparticles had
 greater negative effects on species with smaller cell sizes, such that
 nanoparticle pollution weakened the competitive dominance of smaller
 species and promoted species diversity. Moreover, nanoparticles reduced
 the growth rate differences and competitive ability differences of
 competing species, while having little effect on species niche
 differences. Consequently, nanoparticle pollution reduced the selection
 effect on phytoplankton community abundance but increased the selection
 effect on community biomass. Our results suggest cell size as a key
 functional trait to consider when predicting phytoplankton community
 structure and ecosystem functioning in the face of increasing
 nanopollution.
description: # Cell size-dependent species sensitivity to nanoparticles underlies
 changes in phytoplankton diversity and productivity
 [https://doi.org/10.5061/dryad.02v6wwq92](https://doi.org/10.5061/dryad.02v6wwq92) ## Description of the data and file structure 1. File list: A) phytoplankton volume.csv B) monoculture.csv C) bi-species culture.csv D) invasion.csv 2. Data-specific information for: phytoplankton volume.csv **Number of variables:** 3 **Number of rows:** 150 **Variable List: ** replicate: number of individuals being measured species: the abbreviations of the species in this study volume: the cell volume of single individual, which unit is μm^3 per cell 3. Data-specific information for: monoculture.csv **Number of variables:** 6 **Number of rows:** 898 **Variable List: ** species: the abbreviations of the species in this study day: the time after the start of monocultural incubation NPs: nanoparticle concentration, which unit is mg per L rep: replicate number cell_per_ml: the density of monocultures, which unit is the number of individuals (or cells) per ml biomass_per_ml: the biomass of monocultures, which unit is pg carbon per mL 5. Data-specific information for: bi-species culture.csv **Number of variables:** 16 **Number of rows:** 1800 **Variable List: ** day: the time after the start of bi-species culture combination: the species component of bi-species cultures NPs: nanoparticle concentration, which unit is mg per L replicate: replicate number ANK_cell_ml: the density of species ANK in the bi-species culture (if contain), which unit is number of individuals (or cells) per ml CHLA_cell_ml: the density of species CHLA in the bi-species culture (if contain), which unit is number of individuals (or cells) per ml SCE_cell_ml: the density of species SCE in the bi-species culture (if contain), which unit is number of individuals (or cells) per ml SEL_cell_ml: the density of species SEL in the bi-species culture (if contain), which unit is number of individuals (or cells) per ml STA_cell_ml: the density of species STA in the bi-species culture (if contain), which unit is number of individuals (or cells) per ml ANK_biomass_ml: the biomass of species ANK in the bi-species culture (if contain), which unit is pg carbon per mL CHLA_biomass_ml: the biomass of species CHLA in the bi-species culture (if contain), which unit is pg carbon per mL SCE_biomass_ml: the biomass of species SCE in the bi-species culture (if contain), which unit is pg carbon per mL SEL_biomass_ml: the biomass of species SEL in the bi-species culture (if contain), which unit is pg carbon per mL STA_biomass_ml: the biomass of species STA in the bi-species culture (if contain), which unit is pg carbon per mL cell_ml: the density of the bi-species community (i.e., the sum of the densities of the two component species), which unit is number of individuals (or cells) per ml biomass_ml: the biomass of the bi-species community (i.e., the sum of the biomasses of the two component species), which unit is pg carbon per mL 7. Data-specific information for: invasion.csv **Number of variables:** 10 **Number of rows:** 1196 **Variable List: ** combination: the species component of bi-species cultures day: the time after adding the invasive species to the stabilized residential species NPs: nanoparticle concentration, which unit is mg per L replicate: replicate number residential_cell_ml: the density of residential species, which unit is number of individuals (or cells) per ml residential_biomass_ml: the biomass of residential species, which unit is pg carbon per mL invader_cell_ml: the density of invasive species, which unit is number of individuals (or cells) per ml invader_biomass_ml: the biomass of invasive species, which unit is pg carbon per mL residential_sp: the abbreviated name of residential species invasive_sp: the abbreviated name of invasive species ## Sharing/Access information This is a section for linking to other ways to access the data, and for linking to sources the data is derived from, if any. Links to other publicly accessible locations of the data: * [http://...](http://...) Data was derived from the following sources: ## Code/Software This is an optional, freeform section for describing any code in your submission and the software used to run it. Describe any scripts, code, or notebooks (e.g., R, Python, Mathematica, MatLab) as well as the software versions (including loaded packages) that you used to run those files. If your repository contains more than one file whose relationship to other scripts is not obvious, provide information about the workflow that you used to run those scripts and notebooks.