DOI: 10.5061/dryad.ffbg79d36
title: Data from: Developmental polychlorinated biphenyl (PCB) exposure impacts on voiding physiology persist into adulthood and influence sensitivity to bladder stimuli in mice

description: Polychlorinated biphenyls (PCBs) are toxicants present in the environment
 and are linked to numerous adverse health effects; however, impacts of
 developmental PCB exposure on lower urinary tract function are
 understudied. We have previously found developmental exposure (in utero
 and lactational) to a human-relevant PCB mixture in mice leads to sex- and
 dose- dependent changes to urinary voiding physiology at 6 weeks of age.
 This study expands upon previous findings to investigate if developmental
 PCB-induced urinary voiding phenotypes persist or shift as mice age to 12
 weeks of age. Urinary voiding physiology testing through void spot assays,
 uroflowmetry, and cystometry demonstrated several sex- and dose- dependent
 effects of PCB exposure at 12 weeks of age. This study demonstrates
 developmental exposure to PCBs continues to impact lower urinary tract
 function in adulthood to at least 12 weeks of age both during homeostatic
 conditions and upon challenge of capsaicin in mice. Better understanding
 of how early life stressors like PCBs contribute to aging-associated
 voiding dysfunction are imperative as these findings may help mitigate
 risk or improve treatment strategies for patients suffering from lower
 urinary tract symptoms. This dataset provides the raw data for
 mouse void spot assays, uroflowmetry, cystometry and tissue mass endpoints
 for manuscript: "Developmental Polychlorinated Biphenyl (PCB)
 Exposure Impacts on Voiding Physiology Persist into Adulthood and
 Influence Sensitivity to Bladder Stimuli in Mice". The data is
 provided in an excel speadsheet and each tab of the spreadsheet represents
 the raw data from each endpoint assessed including void spot assay,
 uroflowmetry, cystometry, cystometry with capsaicin, and tissue and body
 mass. These data correspond to each figure in the manuscript. The data are
 organized by PCB treatment group and littermates are entered into
 subcolumns within each treatment group to allow for nesting of the data to
 account for litter effects. 
description: # Developmental polychlorinated biphenyl (PCB) exposure impacts on voiding
 physiology persist into adulthood and influence sensitivity to bladder
 stimuli in mice
 [https://doi.org/10.5061/dryad.ffbg79d36](https://doi.org/10.5061/dryad.ffbg79d36) This dataset provides the raw data in excel format collected for all endpoints presented in the above named manuscript for void spot assay, uroflow, cystometry with saline, cystometry with saline versus capsaicin and mouse tissue wet weights of prostate, seminal vesicle, and testes for mice developmentally exposed to PCBs and collected at 12 weeks of age.  ## Description of the data and file structure Mouse offspring which were developmentally exposed to PCBs underwent voiding physiology testing and assessment of tissue mass. The data for each endpoint examined are in the excel sheet with male and female data labeled, and the title of the endpoint labeled at the top of each data set. Each tab of the excel sheet is an assay and corresponds to the Figures of the manuscript. * First tab of excel sheet (Void Spot Assay): All of the endpoints assessed from the Void spot assay. Endpoints examined include, total spot count, total urine area, area in center, area in corners. These endpoints are labeled in column B and organized with male mice first then the same order repeated again for female mouse data. For each of these endpoints data are labeled in the columns for PCB dose: including a vehicle (0), 0.1 ,1and 6 mg/kg/d PCB treatment groups. Values for each mouse are entered into subcolumns (nested format) within each PCB dose group by litter which is marked in the subcolumn headings, this way litter can be accounted for as a variable. - Second tab of excel sheet (VSA spot size distribution): Void spot assay data that characterizes the void spot size distribution. Data are arranged such that column A is the PCB treatment group, column B is the animal number, column C is the litter that animal comes from, column D is the urine spot size, column E is the raw number of spots of the corresponding spot size. This is for male mice. The same organization follows for columns I-M but for female mice.  * Third tab of the excel sheet (Uroflowmetry) : All of the endpoints assessed from uroflowmetry assay. Endpoints examined include urine void mass, urine void duration, urine flow rate and stream rating. These endpoints are labeled in column B and organized with male mice first then the same order repeated again for female mouse data. For each of these endpoints data are labeled for PCB dose: including a vehicle (0), 0.1,1 and 6 mg/kg/d PCB treatment groups. Values for each mouse are entered into subcolumns (nested format) within each PCB dose group by litter which is marked in the subcolumn headings, this way litter can be accounted for as a variable. - Fourth tab of the excel sheet (CMG Saline only): All of the endpoints assessed from saline only cystometry assay. Endpoints examined include void duration, intervoid interval, threshold pressure, maximum pressure, non-void contractions and compliance. These endpoints are labeled in column B and organized with male mice first then the same order repeated again for female mouse data. For each of these endpoints data are labeled for PCB dose: including a vehicle (0), 0.1,1 and 6 mg/kg/d PCB treatment groups. Values for each mouse are entered into subcolumns (nested format) within each PCB dose group by litter which is marked in the subcolumn headings, this way litter can be accounted for as a variable. * Fifth tab of the excel sheet (CMG saline vs capsaicin): All of the endpoints assessed from comparison of saline and capsaicin cystometry with those two treatments compared in the same animal as a before/after design. Endpoints examined include void duration, threshold pressure, maximum pressure, non-void contractions, intervoid interval, and compliance. These endpoints are labeled in column A and organized with male mice first then the same order repeated again for female mouse data. For each of these endpoints data are labeled for PCB dose: including a vehicle (0), 0.1,1 and 6 mg/kg/d PCB treatment groups across columns. For each PCB treament group subcolumns are labeled as treatment (saline or capsaicin), animal, litter, and value for the raw number for that endpoint.  - Sixth tab of the excel sheet (Body and tissue mass): All of the endpoints assessed from body or tissue mass. Endpoints examined include male body mass, female body mass, male anterior prostate, anterior prostate mass/body mass, ventral prostate mass, ventral prostate mass/body mass, dorsal prostate, dorsal prostate/body mass, testes mass, testes mass/body mass, seminal vesicle mass, and seminal vesicle mass/body mass. These endpoints are labeled in column B. For each of these endpoints data are labeled for PCB dose: including a vehicle (0), 0.1,1 and 6 mg/kg/d PCB treatment groups across columns. Values for each mouse are entered into subcolumns (nested format) within each PCB dose group by litter which is marked in the subcolumn headings, this way litter can be accounted for as a variable.