cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14269551.v1
title: Spatial and temporal variation of the phytoplankton structure in cascading oligotrophic reservoirs of southeast Brazil

description: Abstract: Aim Structural changes in phytoplankton was evaluated in one ultraoligotrophic and two oligotrophic cascading reservoirs during two climatic periods (austral winter and summer) of 2014. Changes in phytoplankton biomass, species composition, and codons (functional groups) were evaluated along a vertical and a longitudinal profile. Methods Four sampling sites were selected for the first reservoir in the cascade (Cachoeira do França) and three for each of the other two reservoirs (Cachoeira da Fumaça and Serraria). Samples were collected along a vertical and a longitudinal profile. Species biomass and codons ‘sensu’ Reynolds were identified and expressed in terms of biomass. Results 18 codons were identified and associated to the reservoirs’ limnological conditions. Composition and biomass of descriptor species changed among seasons. Codon B consisting mainly of Discostella stelligera was the most frequent in all reservoirs in both climatic seasons. Conclusions Our findings showed that seasonality was the key factor for the species composition and phytoplankton codons changes. However, the cascade effect on phytoplankton structure was shown by the continuous contribution of Ceratium furcoides (codon LM) during the winter, and of Discostella stelligera (codon B) in both sampling periods in all three reservoirs.
DOI: 10.6084/m9.figshare.14269552.v1
title: Record of parasitic oomycetes on neotropical copepods in aquatic environments of Northwestern Patagonia (Argentina)

description: Abstract: Aim Oomycetes are one of the most widespread and destructive parasitic groups in the world. The first infection of oomycetes on copepod Parabroteas sarsi Daday 1901 was recently recorded in America. Oomycetes infection on this species makes their eggs unviable and could differentially affect the survival of females. The aim of this study was to track the incidence of oomycetes in two populations of P. sarsi. Methods Two Patagonian ponds were monitored during spring-summer of 2014-2018, measuring environmental variables and the prevalence of oomycete infections on P. sarsi populations. Taxonomic determination of the oomycetes was performed in laboratory. Sex ratios also were recorded in each survey. Results The presence of Aphanomyces ovidestruens Gicklhorn 1923 and Pythium flevoense Van der Plaats-Niterink 1972 was detected in females of P. sarsi in both ponds. We observed a decrease in the parasite prevalence over time, from ~25% (assessed on the total number of adults) to almost disappear completely. Besides, a lower proportion of females than males were observed in samples with high prevalence of parasitism. No temperature differences were found during the sampling years and the monthly temperatures between the maximum and minimum prevalences were similar. Conclusions Our study presents a new record of oomycetes parasitizing copepods in America. Temperature can play a key role in the dynamics of parasite species, but in our study this variable not affected the incidence of the infection. However, other variables could be affect the pattern of the infection such as the presence of predators of oomycetes spores (cladocerans). The lower females proportion when the infection was higher, could support the idea of differential sex/mortality induced by the infection. Recording and identification of parasites is a critical starting point for wildlife management, which will provide an early warning to prevent the spread of harmful parasites such as oomycetes.
DOI: 10.6084/m9.figshare.6857108
title: Assessment of a segregated medicated early weaning protocol for elimination of Actinobacillus pleuropneumoniae

description: ABSTRACT: To assess the success of a segregated medicated early weaning protocol for elimination of Actinobacillus pleuropneumoniae, ten consecutive batches of 100 pigs per batch weaned at 7-9 days of age were monitored at 21-22 weeks of age looking for presence of productive cough, presence of antibodies against the agent and pleuropneumonia-compatible lung lesions at slaughter. Also, whole tonsils were processed for A. pleuropneumoniae identification by both bacteriological isolation and nested-PCR using a direct method. Positive serological test (1.4%) and PCR (3.5%) results, suggested that A. pleuropneumoniae could not be eradicated in all batches, even using segregated medicated early weaning protocol.
DOI: 10.6084/m9.figshare.6857108.v1
title: Assessment of a segregated medicated early weaning protocol for elimination of Actinobacillus pleuropneumoniae

description: ABSTRACT: To assess the success of a segregated medicated early weaning protocol for elimination of Actinobacillus pleuropneumoniae, ten consecutive batches of 100 pigs per batch weaned at 7-9 days of age were monitored at 21-22 weeks of age looking for presence of productive cough, presence of antibodies against the agent and pleuropneumonia-compatible lung lesions at slaughter. Also, whole tonsils were processed for A. pleuropneumoniae identification by both bacteriological isolation and nested-PCR using a direct method. Positive serological test (1.4%) and PCR (3.5%) results, suggested that A. pleuropneumoniae could not be eradicated in all batches, even using segregated medicated early weaning protocol.
DOI: 10.6084/m9.figshare.6857600
title: Moisture content on the mechanical behavior of crambe grains

description: ABSTRACT: This study aimed to verify the influence of moisture content on the values of maximum compressive strength, proportional deformity modulus, elastic coefficients d, e, and f beyond the tangent values, and maximum grain drying of crambe (Crambe abyssinica Hochst) under compression in natural resting position. Crambe grains with moisture contents ranging from 0.1547 to 0.0482 decimal db were dried at 40°C and uniaxially compressed between two parallel plates in the natural rest position. The gravimetric method was used to monitor reduction in moisture content during drying (weight loss). It was concluded that the compression force required to deform the crambe grains decreases as the moisture content increases. The proportional deformity modulus increases as the deformation decreases, yielding values between (0.09-0.27)×102MPa. Sigmoidal model described by Taylor series adequately represents the compression resistance of crambe grains in the natural rest position with moisture content lower than 0.0813db.
DOI: 10.6084/m9.figshare.14269551
title: Spatial and temporal variation of the phytoplankton structure in cascading oligotrophic reservoirs of southeast Brazil

description: Abstract: Aim Structural changes in phytoplankton was evaluated in one ultraoligotrophic and two oligotrophic cascading reservoirs during two climatic periods (austral winter and summer) of 2014. Changes in phytoplankton biomass, species composition, and codons (functional groups) were evaluated along a vertical and a longitudinal profile. Methods Four sampling sites were selected for the first reservoir in the cascade (Cachoeira do França) and three for each of the other two reservoirs (Cachoeira da Fumaça and Serraria). Samples were collected along a vertical and a longitudinal profile. Species biomass and codons ‘sensu’ Reynolds were identified and expressed in terms of biomass. Results 18 codons were identified and associated to the reservoirs’ limnological conditions. Composition and biomass of descriptor species changed among seasons. Codon B consisting mainly of Discostella stelligera was the most frequent in all reservoirs in both climatic seasons. Conclusions Our findings showed that seasonality was the key factor for the species composition and phytoplankton codons changes. However, the cascade effect on phytoplankton structure was shown by the continuous contribution of Ceratium furcoides (codon LM) during the winter, and of Discostella stelligera (codon B) in both sampling periods in all three reservoirs.
DOI: 10.6084/m9.figshare.14269561
title: Phenological Analysis of Gymnanthes Klotzschiana Müll.Arg Belonging to Two Atlantic Forest Biome Formations

description: Abstract Gymnanthes klotzschiana is a tree native to the Atlantic Forest Biome. The objective of this study was to analyze the synchronization of five phenophases and their correlation with environmental factors during two years of survey in two distinct areas (Area 1: Mixed Alluvial Ombrophilous Forest; Area 2: Montane Semi-deciduous Seasonal Forest). Ten individuals were randomly selected in each area, with the following variables being studied: leaf fall and budding, flowering, and immature and ripe fruit production. The activity index and the intensity index were determined for each phenological event. Timing and interactions with environmental factors were tested using Spearman’s correlation. The photoperiod correlated with all phenophases and precipitation did not correlate with any. There was synchronization in the foliar fall and foliage in the two areas. The species can be characterized as semi-deciduous. Flowering and fruiting were annual, with the highest intensity of fruit production occurring in Area 1.
DOI: 10.6084/m9.figshare.14269561.v1
title: Phenological Analysis of Gymnanthes Klotzschiana Müll.Arg Belonging to Two Atlantic Forest Biome Formations

description: Abstract Gymnanthes klotzschiana is a tree native to the Atlantic Forest Biome. The objective of this study was to analyze the synchronization of five phenophases and their correlation with environmental factors during two years of survey in two distinct areas (Area 1: Mixed Alluvial Ombrophilous Forest; Area 2: Montane Semi-deciduous Seasonal Forest). Ten individuals were randomly selected in each area, with the following variables being studied: leaf fall and budding, flowering, and immature and ripe fruit production. The activity index and the intensity index were determined for each phenological event. Timing and interactions with environmental factors were tested using Spearman’s correlation. The photoperiod correlated with all phenophases and precipitation did not correlate with any. There was synchronization in the foliar fall and foliage in the two areas. The species can be characterized as semi-deciduous. Flowering and fruiting were annual, with the highest intensity of fruit production occurring in Area 1.
DOI: 10.6084/m9.figshare.6857600.v1
title: Moisture content on the mechanical behavior of crambe grains

description: ABSTRACT: This study aimed to verify the influence of moisture content on the values of maximum compressive strength, proportional deformity modulus, elastic coefficients d, e, and f beyond the tangent values, and maximum grain drying of crambe (Crambe abyssinica Hochst) under compression in natural resting position. Crambe grains with moisture contents ranging from 0.1547 to 0.0482 decimal db were dried at 40°C and uniaxially compressed between two parallel plates in the natural rest position. The gravimetric method was used to monitor reduction in moisture content during drying (weight loss). It was concluded that the compression force required to deform the crambe grains decreases as the moisture content increases. The proportional deformity modulus increases as the deformation decreases, yielding values between (0.09-0.27)×102MPa. Sigmoidal model described by Taylor series adequately represents the compression resistance of crambe grains in the natural rest position with moisture content lower than 0.0813db.
DOI: 10.6084/m9.figshare.14269564
title: Imbibition, Germination and Vigor of Gymnanthes Klotzschiana Müll.Arg. Seeds Under Hypoxia

description: Abstract The objective of this study was to assess hypoxia tolerance in Gymnanthes klotzschiana seeds harvested in two areas (Area 1 - subject to flooding; and Area 2 - not subject to flooding) by the imbibition curve, vigor and germination. Seeds of the two areas were submitted to: one (T-1d), three (T-3d), five (T-5d), ten (T-10d) and twenty days under hypoxia (T-20d); in addition to the control (T-0d). All treatments presented three germination stages. The highest germination was achieved in Area 1 T-1d (90%) and the lowest in Area 2 T-20d (31%). The Area 1 seeds required less time and water content to finish their germination, and they presented greater tolerance to hypoxia; a fact proven by the germination and vigor results. G. klotzschiana seeds harvested in an area subject to flooding are more tolerant to hypoxia and are recommended for restoration projects in this type of area.
DOI: 10.6084/m9.figshare.14269564.v1
title: Imbibition, Germination and Vigor of Gymnanthes Klotzschiana Müll.Arg. Seeds Under Hypoxia

description: Abstract The objective of this study was to assess hypoxia tolerance in Gymnanthes klotzschiana seeds harvested in two areas (Area 1 - subject to flooding; and Area 2 - not subject to flooding) by the imbibition curve, vigor and germination. Seeds of the two areas were submitted to: one (T-1d), three (T-3d), five (T-5d), ten (T-10d) and twenty days under hypoxia (T-20d); in addition to the control (T-0d). All treatments presented three germination stages. The highest germination was achieved in Area 1 T-1d (90%) and the lowest in Area 2 T-20d (31%). The Area 1 seeds required less time and water content to finish their germination, and they presented greater tolerance to hypoxia; a fact proven by the germination and vigor results. G. klotzschiana seeds harvested in an area subject to flooding are more tolerant to hypoxia and are recommended for restoration projects in this type of area.
DOI: 10.6084/m9.figshare.14269570
title: Frequency of microorganisms in vaginal discharges of high-risk pregnant women from a hospital in Caruaru, Pernambuco, Brazil

description: ABSTRACT Introduction: During pregnancy, estrogen and progesterone levels may favor vaginal colonization by pathogenic microorganisms that can be associated with obstetric complications and neonatal infections. These pathogens include Candida spp., Trichomonas vaginalis, Gardnerella vaginalis and Streptococcus agalactiae. Objective: To determine the frequency of such microorganisms amongst high-risk pregnant women receiving prenatal care at a hospital in Caruaru, Pernambuco. Material and method: Analytical study in which samples of vaginal discharge were collected from women experiencing high-risk pregnancies, without restriction of age or gestational period. The samples were submitted to Gram-stained direct smear, as well as to fresh wet-mount examination and to cultures in Sabouraud and blood agar. Results: From May to December 2018, 92 patients were selected for sampling. The frequency of Candida spp. was found to be 31.52%. Concerning Gardnerella vaginalis, the frequency was 1.25%. The rate of colonization by Streptococcus agalactiae was 3.23%. No cases of Trichomonas vaginalis were found in this study. Discussion: The frequency of Candida that was found corroborates the literature, as it is a common infection during pregnancy due to hormonal increase. However, the frequencies of colonization by G. vaginalis, T. vaginalis and S. agalactiae were lower than those found in other studies. Conclusion: Due to the risks that these microorganisms can bring to a pregnant woman and a fetus, health professionals should be alert to signs and symptoms, requesting the screening of these pathogens, as well as treating gestating women when necessary.
DOI: 10.6084/m9.figshare.14269573.v1
title: Evaluation of quality indicators of cervical cytopathology tests carried out in a municipality of Paraná, Brazil

description: ABSTRACT Introduction: Although the criteria and recommendations are well defined to improve the quality of cervical cytopathology tests (Pap smear) in Brazil, the quality indicators of several laboratories are below the recommended standards. Objective: To evaluate the quality indicators of cervical cytopathology tests carried out by the Brazilian Unified Health System [Sistema Único de Saúde (SUS)]. Method: Cross-sectional study based on the results of cervical cytopathology tests conducted in the municipality of Cascavel, Paraná, Brazil, from January 2012 to December 2018. The study included 141,191 tests and the following variables were analyzed: sample suitability, cervical cytopathology tests result, and internal quality monitoring indicators recommended by the Brazilian Ministry of Health. Results: The percentage of unsatisfactory tests did not exceed 0.97%. The representation of the squamocolumnar junction ranged from 63% to 67.4%. The percentage of altered exams among the satisfactory samples ranged from 0.46% to 6.44%. The positivity index ranged from 0.46% to 6.44%, while the high-grade squamous intraepithelial lesion/satisfactory indicator ranged from 0.21% to 1.06%, atypical squamous cells of undetermined significance/altered ranged from 22.47% to 49.93%, atypical squamous cells of undetermined significance/satisfactory ranged from 0.1% to 2.57%: and atypical squamous cells of undetermined significance/squamous intraepithelial lesions ranged from 0.31 to 1.02. Conclusion: In 2012 and 2013, the high-grade squamous intraepithelial lesion/satisfactory and the positivity index indicators were below the recommended parameters, and in the remaining years, these indicators were within the parameters recommended. Consequently, a greater number of truly cancer precursor lesions were identified, demonstrating the importance of internal quality control in the prevention of cervical cancer.
DOI: 10.6084/m9.figshare.6857585
title: Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

description: ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (&gt;90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.
DOI: 10.6084/m9.figshare.6857585.v1
title: Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

description: ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (&gt;90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.
DOI: 10.6084/m9.figshare.14269581
title: Deglutition manifestations in patients with oropharyngeal cancer subjected to conservative therapy: systematic review

description: ABSTRACT Objectives: To characterize scientific production and identify deglutition changes in individuals with oropharyngeal cancer subjected to conservative therapy. Methods: The search was applied to five electronic database [Scientific Electronic Library Online (Scielo), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine National Institutes of Health (PubMed), Web of Science, and Scopus], besides the search of grey literature in the databases (OpenThesis e OpenGrey), avoiding selection and publication bias. Prospective longitudinal studies concerning the theme: deglutition disorders in individuals with oropharyngeal cancer subjected to conservative therapy were considered eligible. The risk of bias and the evaluation of individual methodological quality of the selected studies were measured by “The Joanna Briggs Institute Critical Appraisal tools for use in JBI Systematic Reviews” for prospective and longitudinal studies. Results: The search resulted in 899 records and after analysis four of them fulfilled the eligibility criteria. Among the studies included, all individuals presented some type of deglutition changes, the most common were: reduced of strength and retraction of the base of the tongue, delayed deglutition trigger, reduced laryngeal elevation, presence of residues on tongue and palate, in the pharyngeal area, valleculae, and posterior pharyngeal wall, as well as in the vestibules and in pyriform sinuses. Conclusion: The evidence from this systematic review suggests that conservative therapies cause deglutition changes or amplify the pre-existing ones, regardless of the type and magnitude of radiation, as well as tumor staging. However, there is little standardization in the research methodologies, making a meta-analysis study difficult to conduct.
DOI: 10.6084/m9.figshare.14269581.v1
title: Deglutition manifestations in patients with oropharyngeal cancer subjected to conservative therapy: systematic review

description: ABSTRACT Objectives: To characterize scientific production and identify deglutition changes in individuals with oropharyngeal cancer subjected to conservative therapy. Methods: The search was applied to five electronic database [Scientific Electronic Library Online (Scielo), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine National Institutes of Health (PubMed), Web of Science, and Scopus], besides the search of grey literature in the databases (OpenThesis e OpenGrey), avoiding selection and publication bias. Prospective longitudinal studies concerning the theme: deglutition disorders in individuals with oropharyngeal cancer subjected to conservative therapy were considered eligible. The risk of bias and the evaluation of individual methodological quality of the selected studies were measured by “The Joanna Briggs Institute Critical Appraisal tools for use in JBI Systematic Reviews” for prospective and longitudinal studies. Results: The search resulted in 899 records and after analysis four of them fulfilled the eligibility criteria. Among the studies included, all individuals presented some type of deglutition changes, the most common were: reduced of strength and retraction of the base of the tongue, delayed deglutition trigger, reduced laryngeal elevation, presence of residues on tongue and palate, in the pharyngeal area, valleculae, and posterior pharyngeal wall, as well as in the vestibules and in pyriform sinuses. Conclusion: The evidence from this systematic review suggests that conservative therapies cause deglutition changes or amplify the pre-existing ones, regardless of the type and magnitude of radiation, as well as tumor staging. However, there is little standardization in the research methodologies, making a meta-analysis study difficult to conduct.