DOI: 10.5061/dryad.0d19278
title: Data from: Light environment change induces differential expression of guppy opsins in a multi-generational evolution experiment

description: Light environments critically impact species that rely on vision to
 survive and reproduce. Animal visual systems must accommodate changes in
 light that occur from minutes to years, yet we do not know how they
 respond to divergent spectral changes over longer time scales. Here we
 used a laboratory experiment where replicate guppy populations were kept
 different light environments for up to 8-12 generations to explore
 possible differences in the expression levels of nine guppy opsin genes.
 Previous evidence for opsin expression-light environment ‘tuning’ has been
 either correlative or focused exclusively on the relationship between the
 light environment and opsin expression over one or two generations. In our
 multi-generation experiment, the relative expression levels of nine
 different opsin genes responded differently to light environment changes:
 some did not respond, while others differed due to phenotypic plasticity.
 Moreover for LWS-1 we found that, while we observed a wide range of
 plastic responses under different light conditions, common plastic
 responses occurred only after multigenerational exposure to different
 light environments. Taken together this suggests that opsin expression
 plasticity plays an important role in light environment ‘tuning’ in
 different light environments and, in turn, has important implications for
 both visual system function and evolution.
description: Guppy digital PCR
 concentrationsThis file contains the
 absolute quantification values for four Poecila reticulata transcripts
 that accompanies: Kranz, A. M., Forgan L. G., Cole G. L., Endler, J. A.
 2018. Light environment change induces differential expression of guppy
 opsins in a multi-generational evolution experiment. Absolute
 quantification of target transcripts was determined by digital PCR, using
 EvaGreen Supermix (Bio-Rad) and the QX 200™ Droplet Digital™ PCR system
 (Bio-Rad). The absolute quantification of each transcript was calculated
 using the Quantasoft v1.7.4 (Bio-Rad) and is represented as copies of
 transcript per microliter of amplified PCR mixture. Tank = T-numbers refer
 to tank numbers. Treatment = EV, evolution experiment (filtered light
 conditions); AP, adult plasticity experiment; DP, developmental plasticity
 experiment. Sampling date = Sampling month and year. Light environment =
 the populations’ designated light environment. Green (F89) filtered light;
 Clear (CF) filtered light Genes of interest = LWS-1, LWS-3, RH2-2 and
 EF-1a.dPCR
 concentrations.xlsxRelative qPCR expression
 (CNRQ) values for nine guppy opsinsThis file contains the
 calibrated normalized relative quantity (CNRQ) values for nine Poecila
 reticulata opsin transcripts that accompanies: Kranz, A. M., Forgan L. G.,
 Cole G. L., Endler, J. A. 2018. Light environment change induces
 differential expression of guppy opsins in a multi-generational evolution
 experiment. Calibrated normalized relative quantity (CNRQ) values were
 calculated using qBase+ software (version 3). Tank = T-numbers refer to
 tank numbers. Treatment = EV, evolution experiment (filtered light
 conditions); AP, adult plasticity experiment; DP, developmental plasticity
 experiment. Sampling date = Sampling month and year. Light environment =
 the populations’ designated light environment. Green (F89) filtered light;
 Lilac (F55) filtered light; Clear (CF) filtered light. Genes of interest
 are LWS-1, LWS-2, LWS-3, LWS-4, RH2-1, RH2-2, SWS1, SWS2a,
 SWS2b.qPCR
 values.xlsxEvolution experiment
 qPCR CT values for 11 genesThis file contains the
 quantitative real time RT-PCR (qPCR) cycle threshold values for nine
 Poecila reticulata opsin genes and two references genes that accompanies:
 Kranz, A. M., Forgan L. G., Cole G. L., Endler, J. A. 2018. Light
 environment change induces differential expression of guppy opsins in a
 multi-generational evolution experiment. Cycle threshold values were
 determined by qPCR using SYBR® Premix Ex Taq Master Mix (Takara) on a
 Corbett Rotor-Gene 3000 (Corbett Research). For each sample, T-numbers
 refer to tank numbers and EV refers to the evolution experiment under
 filtered light conditions. UNKN, Unknown; STD, standard dilution series;
 NTC, no temple control; Cal, calibrator. Pr, Poecilia reticulata.
 Reference genes are ActinB and EF-1a. Genes of interest are LWS-1, LWS-2,
 LWS-3, LWS-4, RH2-1, RH2-2, SWS1, SWS2a, SWS2b.qPCR CT values_EV
 experiment_11genes.xlsxAdult plasticity
 experiment qPCR CT values for 11 genesThis file contains the
 quantitative real time RT-PCR (qPCR) cycle threshold values for nine
 Poecila reticulata opsin genes and two references genes that accompanies:
 Kranz, A. M., Forgan L. G., Cole G. L., Endler, J. A. 2018. Light
 environment change induces differential expression of guppy opsins in a
 multi-generational evolution experiment. Cycle threshold values were
 determined by qPCR using SYBR® Premix Ex Taq Master Mix (Takara) on a
 Corbett Rotor-Gene 3000 (Corbett Research). For each sample, T-numbers
 refer to tank numbers and AP refers to the adult plasticity experiment
 under lab light conditions. UNKN, Unknown; STD, standard dilution series;
 NTC, no temple control; Cal, calibrator. Pr, Poecilia reticulata.
 Reference genes are ActinB and EF-1a. Genes of interest are LWS-1, LWS-2,
 LWS-3, LWS-4, RH2-1, RH2-2, SWS1, SWS2a, SWS2b.qPCR CT values_AP
 experiment_11genes.xlsxDevelopmental plasticity
 experiment qPCR CT values for 11 genesThis file contains the
 quantitative real time RT-PCR (qPCR) cycle threshold values for nine
 Poecila reticulata opsin genes and two references genes that accompanies:
 Kranz, A. M., Forgan L. G., Cole G. L., Endler, J. A. 2018. Light
 environment change induces differential expression of guppy opsins in a
 multi-generational evolution experiment. Cycle threshold values were
 determined by qPCR using SYBR® Premix Ex Taq Master Mix (Takara) on a
 Corbett Rotor-Gene 3000 (Corbett Research). For each sample, T-numbers
 refer to tank numbers and DP refers to the developmental plasticity
 experiment under lab light conditions. UNKN, Unknown; STD, standard
 dilution series; NTC, no temple control; Cal, calibrator. Pr, Poecilia
 reticulata. Reference genes are ActinB and EF-1a. Genes of interest are
 LWS-1, LWS-2, LWS-3, LWS-4, RH2-1, RH2-2, SWS1, SWS2a,
 SWS2b.qPCR
 CT values_DP experiment_11genes.xlsx