DOI: 10.5061/dryad.q86q0
title: Data from: Context-dependent effects of Y chromosome and mitochondrial haplotype on male locomotive activity in Drosophila melanogaster

description: Some regions of the genome exhibit sexual asymmetries in inheritance, and
 are thus subjected to sex-biased evolutionary forces. Maternal inheritance
 of mitochondrial DNA (mtDNA) enables mtDNA mutations harmful to males, but
 not females, to accumulate. In the face of male-harmful mtDNA mutation
 accumulation, selection will favour the evolution of compensatory
 modifiers in the nuclear genome that offset fitness losses to males. The Y
 chromosome is a candidate to host these modifiers, because it is
 paternally-inherited, known to harbour an abundance of genetic variation
 for male fertility, and therefore likely to be under strong selection to
 uphold male viability. Here, we test for inter-genomic interactions
 involving mtDNA and Y chromosomes in male Drosophila melanogaster.
 Specifically, we examine effects of each of these genomic regions, and
 their interaction, on locomotive activity, across different environmental
 contexts – both dietary and social. We found that both the mtDNA haplotype
 and Y chromosome haplotype affected activity in males assayed in an
 environment perceived as social. These effects, however, were not evident
 in males assayed in perceived solitary environments, and neither social
 nor solitary treatments revealed evidence for inter-genomic interactions.
 Finally, the magnitude and direction of these genetic effects was further
 contingent on the diet treatment of the males. Thus, genes within the
 mtDNA and Y chromosome are involved in genotype-by-environment
 interactions. These interactions might contribute to the maintenance of
 genetic variation within these asymmetrically-inherited gene regions and
 complicate the dynamics of genetic interactions between the mtDNA and the
 Y chromosome.
description: Context-dependent
 effects of Y chromosome and mitochondrial haplotype on male locomotive
 activity in Drosophila melanogasterID: Identifies
 Mito_Y_Dup_Diet where C=yeast and S=agar. Sample and Run: ID is split up
 into Sample and Run. Plate: Two different plates were used in the
 experiment. Hour: Broadly defines the time the assay was run.
 MinsAftLight: More specifically defines the time the assay was run.
 SocialEnviro: Describes the two different social contexts the assays were
 run under. Block: Describes the different sets of grandparental flies
 which the focal males descended from. ParentalAge: Describes the age of
 the parents when laying the eggs that grew into the focal males. Distmm:
 Distance travelled by flies in the 30 mins assay, in mm. emptyTime: Time
 when the software did not track the movement of the flies (checking the
 video images manually suggested this was because the flies were
 stationary). Mt = Mitochondrial haplotype, D = Dahomey, M = Madang. Y = Y
 chromosome genotype. Dup: The lines were maintained as two duplicates of
 each Mt and Y substitution. Diet: Focal males were assigned to one of two
 diets after emergence as adults - standard yeast diet (nutrient-rich) and
 a diet of just agar with no added nutrients (nutrient-deprived).
 Block.ParentalAge: Parental age nested within block. MtYDup: Identifies
 the MitoYDuplicate combination.MitoYEnvironment_ActivityData.txt