cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21800456.v1
title: Fig5E_RACE_downstream-primer_R2_LG316.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800459
title: Fig5E_RACE_downstream-primer_R2_LG316_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800459.v1
title: Fig5E_RACE_downstream-primer_R2_LG316_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800462
title: Fig5E_RACE_downstream-primer_R3_LG318.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800462.v1
title: Fig5E_RACE_downstream-primer_R3_LG318.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800465
title: Fig5E_RACE_downstream-primer_R1_LG314.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800465.v1
title: Fig5E_RACE_downstream-primer_R1_LG314.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800468
title: Fig5E_RACE_downstream-primer_R3_LG318.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800468.v1
title: Fig5E_RACE_downstream-primer_R3_LG318.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800471
title: Fig5E_RACE_downstream-primer_R2_LG316.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800426
title: Fig4B_RACE_YKT6_R2_AI10_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800471.v1
title: Fig5E_RACE_downstream-primer_R2_LG316.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800474
title: Fig5E_RACE_downstream-primer_R3_LG318_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800417
title: Fig4B_RACE_STOML2_R2_AI33.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800420
title: Fig4B_RACE_STOML2_R1_AI8_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800420.v1
title: Fig4B_RACE_STOML2_R1_AI8_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800423
title: Fig4B_RACE_STOML2_R3_LG343_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800423.v1
title: Fig4B_RACE_STOML2_R3_LG343_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800426.v1
title: Fig4B_RACE_YKT6_R2_AI10_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800474.v1
title: Fig5E_RACE_downstream-primer_R3_LG318_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800240
title: Fig4B_RACE_STOML2_R1_AI8.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800240.v1
title: Fig4B_RACE_STOML2_R1_AI8.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800249.v1
title: Fig4B_RACE_STOML2_R2_AI33_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800267
title: Fig4B_RACE_STOML2_R3_LG343.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800267.v1
title: Fig4B_RACE_STOML2_R3_LG343.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800417.v1
title: Fig4B_RACE_STOML2_R2_AI33.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800246
title: Fig4B_RACE_STOML2_R1_AI8.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800246.v1
title: Fig4B_RACE_STOML2_R1_AI8.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800249
title: Fig4B_RACE_STOML2_R2_AI33_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800303
title: Fig4B_RACE_YKT6_R2_AI10.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800303.v1
title: Fig4B_RACE_YKT6_R2_AI10.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800309
title: Fig4B_RACE_STOML2_R2_AI33.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800309.v1
title: Fig4B_RACE_STOML2_R2_AI33.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800351
title: Fig4B_RACE_STOML2_R3_LG343.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800351.v1
title: Fig4B_RACE_STOML2_R3_LG343.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800354
title: Fig4B_RACE_YKT6_R3_LG310.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800354.v1
title: Fig4B_RACE_YKT6_R3_LG310.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800375
title: Fig4B_RACE_YKT6_R3_LG310.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800375.v1
title: Fig4B_RACE_YKT6_R3_LG310.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800384
title: Fig4B_RACE_YKT6_R1_AI9.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21800384.v1
title: Fig4B_RACE_YKT6_R1_AI9.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with inserted sequence from the STOML2 or YKT6 gene. Insertion size ranges from 15 bp to 99 bp. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.