cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/4b7dv-q3h28
title: wt_serum_blotfrombehind

description: This is a sample of wt grown in serum bottle that made nanowires.
description: <strong>Tilt Series Date:</strong> 2015-07-26</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Sample Preparation:</strong> The sample was put on the C side (glow discharged side) on the left. The blotting was done from behind on the rt side. Vitrobot was used. All other vitrobot conditions are the same.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-6/rawdata/</p>10006.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-6/rawdata/10006.mrc"
        > <i class="download icon"></i> Download </a></p>

        10006_part61_4.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-6/3dimage_59568/10006_part61_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y68c0-azx08
title: Vibrio cholerae delta TcpB

description: Raw data files of Vibrio cholerae delta TcpB
description: <strong>Tilt Series Date:</strong> 2015-10-16</p>
<strong>Data Taken By:</strong> Andreas Kjaer</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown ON in LB with ampicillin and IPTG, refreshed in LB shortly prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-71/3dimage_73385/</p>10162015_TcpB_10072_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-71/3dimage_73385/10162015_TcpB_10072_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        10162015_TcpB_10072.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-71/rawdata/10162015_TcpB_10072.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xp1zb-29x69
title: Shewanella oneidensis

description: Raw data files of Shewanella oneidensis
description: <strong>Tilt Series Date:</strong> 2019-01-13</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> None</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> This mutant was prepared in the lab of Jeffrey A Gralnick at the University of Minnesota. This mutant was ultimately published in the EMBOJ (Kaplan et al., The EMBOJ, 2019)</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-01-13-53/rawdata/</p>Shewanella_dfliF45.mrc,  Tilt Series (Pixel Size 0.53 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-01-13-53/rawdata/Shewanella_dfliF45.mrc"
        > <i class="download icon"></i> Download </a></p>

        Shewanella_dfliF45_full.rec,  Reconstruction (Pixel Size 2.12 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-01-13-53/3dimage_100742/Shewanella_dfliF45_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.28612424.v2
title: Temporal Dynamics of Macrophage Activation During Salmonella enterica serovar Typhi Infection: An Integrated Transcriptomic and Metabolomic Analysis

description: Salmonella enterica serovar Typhi (S. Typhi) is a major bacteria responsible for foodborne illnesses. This study employed an integrated transcriptomic and metabolomic approach to investigate the response of macrophages infected with S. Typhi over a defined stage (0 h, 2 h, 4 h, 8 h, and 24 h). RNA sequencing (RNA-seq) was used to profile gene expression changes, while untargeted metabolomics characterized metabolic shifts. Gene set enrichment and pathway analyses were conducted to identify key immune and metabolic pathways.
DOI: 10.22002/5t430-eft81
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-08</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-08-4/rawdata/</p>targ04.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-08-4/rawdata/targ04.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ04_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-08-4/3dimage_99493/targ04_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j1kbx-dc369
title: Legionella delta DotO

description: Strain from Yale
description: <strong>Tilt Series Date:</strong> 2019-01-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Jun Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase, broad vitrobot, R2/2 quantifoil 200 mesh copper grid</p>
<strong>Sample Preparation:</strong> 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-43/rawdata/</p>tilt_58.mrc,  Tilt Series (Pixel Size 0.27 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-43/rawdata/tilt_58.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt_58_part61_3.rec,  Reconstruction (Pixel Size 1.08 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-43/3dimage_101064/tilt_58_part61_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m5a5z-cjw91
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-20</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, WT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-58/rawdata/</p>targ47.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-58/rawdata/targ47.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ47_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-58/3dimage_101267/targ47_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f0gzj-7qg41
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-100/rawdata/</p>caulobacter_na1000_wt_101_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-100/rawdata/caulobacter_na1000_wt_101_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_101_af.mrc.mdoc,  813.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-100/rawdata/caulobacter_na1000_wt_101_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y1db4-pg156
title: C. crescentus NA1000 Tad pilus

description: C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
description: <strong>Tilt Series Date:</strong> 2019-08-17</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier and Lujia Zhang</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.</p>
<strong>Sample Preparation:</strong> 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-87/rawdata/</p>caulobacter_na1000_wt_088_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-87/rawdata/caulobacter_na1000_wt_088_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        caulobacter_na1000_wt_088_af.mrc.mdoc,  813.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-87/rawdata/caulobacter_na1000_wt_088_af.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yxr0y-90d36
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-05</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-12/rawdata/</p>targ12.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-12/rawdata/targ12.mrc"
        > <i class="download icon"></i> Download </a></p>

        targ12_full.rec,  Reconstruction (Pixel Size 1.4848 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-12/3dimage_99254/targ12_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yh4ps-xdj09
title: JV9264

description: Raw data files of JV9264
description: <strong>Tilt Series Date:</strong> 2018-09-06</p>
<strong>Data Taken By:</strong> Jun Zhang</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degree and OD 2.5-3.0</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-14/rawdata/</p>JV9264_B111.mrc,  Tilt Series (Pixel Size 0.2654 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-14/rawdata/JV9264_B111.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9264_B111_part55_2.rec,  Reconstruction (Pixel Size 1.0616 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-14/3dimage_97761/JV9264_B111_part55_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a1snb-gbb21
title: Legionella cells for the flagell

description: Raw data files of Legionella cells for the flagell
description: <strong>Tilt Series Date:</strong> 2018-03-13</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were grown in LB overnight.</p>
<strong>Sample Preparation:</strong> Grids were prepared from an overnight LB culture.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-61/rawdata/</p>Legionella_flagella32.mrc,  Tilt Series (Pixel Size 0.39 nm), 767.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-61/rawdata/Legionella_flagella32.mrc"
        > <i class="download icon"></i> Download </a></p>

        Legionella_flagella32_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-61/3dimage_92274/Legionella_flagella32_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2jnas6
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster lucidus",
 "Country is Netherlands",
 "HasCoordinate is true"
 ]
}

 The dataset includes 5 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002218-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/k7aqn-yyb23
title: Vibrio DeltaVC1403

description: Vibrio cholerae strain CO35 (delta VC1403)cytoplasmic chemoreceptor in chemotaxis operon with CheA1(F9 chemotaxis system)
description: <strong>Tilt Series Date:</strong> 2015-01-16</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain generated by Catherine Oikonomou</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown 24 hrs in LB at 30C, diluted 150 µl into 2 ml Ca-HEPES buffer, grown 16 hours at 30C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-01-16-13/rawdata/</p>VibrioCO35setBmanual120012.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-01-16-13/rawdata/VibrioCO35setBmanual120012.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioCO35setBmanual120012_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-01-16-13/3dimage_50844/VibrioCO35setBmanual120012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kgmrd-6dn61
title: TRIM5α decorated HIV-1 tubes

description: TRIM5α tube complexes
description: <strong>Tilt Series Date:</strong> 2015-06-13</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> TRIM5α decorated HIV-1 tubes</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -5.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist and Barbie Pornillos</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Yen-Li (Wes Sundquist Lab)</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-13-1/rawdata/</p>tomography1.mrc,  Tilt Series (Pixel Size 2.6 nm), 3.4 GB  
        
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        tomography1_full.rec,  Reconstruction (Pixel Size 10.4 nm), 445.4 MB  
        
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