DOI: 10.5061/dryad.74bk6
title: Data from: A transgenic quail model that enables dynamic imaging of amniote embryogenesis

description: Embryogenesis is the coordinated assembly of tissues during morphogenesis
 through changes in individual cell behaviors and collective cell
 movements. Dynamic imaging, combined with quantitative analysis, is ideal
 for investigating fundamental questions in developmental biology involving
 cellular differentiation, growth control and morphogenesis. However, a
 reliable amniote model system that is amenable to the rigors of extended,
 high-resolution imaging and cell tracking has been lacking. To address
 this shortcoming, we produced a novel transgenic quail that ubiquitously
 expresses nuclear localized monomer cherry fluorescent protein (chFP). We
 characterize the expression pattern of chFP and provide concrete examples
 of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and
 analyze key morphogenetic events during embryonic stages X to 11.
description: HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 1 of 7.Dorsal view of a fixed
 stage HH11PGK1:H2B-chFP embryo counterstained with DAPI collected using a
 20x objective on a Zeiss 780 confocal microscope and Zen 2011 black
 edition 64 bit software. Total file size is 3.48GB. Part 1 of
 7.HH11
 mCherry Dapi Tiled Z Stack 20x.lsm.001HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 2 of 7.HH11 mCherry Dapi Tiled Z Stack
 20x.lsm.002HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 3 of 7.HH11 mCherry Dapi Tiled Z Stack
 20x.lsm.003HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 4 of 7.HH11 mCherry Dapi Tiled Z Stack
 20x.lsm.004HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 5 of 7.HH11 mCherry Dapi Tiled Z Stack
 20x.lsm.005HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 6 of 7.HH11 mCherry Dapi Tiled Z Stack
 20x.lsm.006HH11 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 7 of 7.HH11 mCherry Dapi Tiled Z Stack
 20x.lsm.007Stage X PGK1:H2B-chFP
 Tiled Z Stack 20x.lsm. Part 1 of 4Dorsal view of an
 un-incubated stage X PGK1:H2B-chFP embryo counterstained with DAPI
 collected using a 20x objective on a Zeiss 780 confocal microscope and Zen
 2011 black edition 64 bit software. Total file size is 2.07GB. Part 1 of
 4.Stage X
 unincubated PGK H2B Cherry 20x 1x tiled Z stack 2
 stiched.lsm.001Stage X PGK1:H2B-chFP
 Tiled Z Stack 20x.lsm. Part 2 of 4.Stage X unincubated PGK H2B
 Cherry 20x 1x tiled Z stack 2
 stiched.lsm.002Stage X PGK1:H2B-chFP
 Tiled Z Stack 20x.lsm. Part 3 of 4.Stage X unincubated PGK H2B
 Cherry 20x 1x tiled Z stack 2
 stiched.lsm.003Stage X PGK1:H2B-chFP
 Tiled Z Stack 20x.lsm. Part 4 of 4.Stage X unincubated PGK H2B
 Cherry 20x 1x tiled Z stack 2
 stiched.lsm.004HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 1 of 7.Dorsal view of a fixed
 stage HH5 PGK1:H2B-chFP embryo counterstained with DAPI collected using a
 20x objective on a Zeiss 780 confocal microscope and Zen 2011 black
 edition 64 bit software. Total file size is 3.50GB. Part 1 of
 7.HH5
 mCherry Dapi 20x Tiled Z
 Stack.lsm.001HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 2 of 7.HH5 mCherry Dapi 20x Tiled Z
 Stack.lsm.002HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 3 of 7.HH5 mCherry Dapi 20x Tiled Z
 Stack.lsm.003HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 4 of 7.HH5 mCherry Dapi 20x Tiled Z
 Stack.lsm.004HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 5 of 7.HH5 mCherry Dapi 20x Tiled Z
 Stack.lsm.005HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 6 of 7.HH5 mCherry Dapi 20x Tiled Z
 Stack.lsm.006HH5 PGK1:H2B-chFP Tiled
 Z Stack 20x.lsm. Part 7 of 7.HH5 mCherry Dapi 20x Tiled Z
 Stack.lsm.007