DOI: 10.5061/dryad.mcvdnck6j
title: Spreadsheets for determining the immunoreactivity of radioimmunoconjugates

description: In the published manuscript for which this data set was created, we
 provide detailed protocols for the measurement of the immunoreactivity of
 radiolabeled antibodies. To provide some context, full-length antibodies
 and smaller antibody fragments have been viewed as promising vectors for
 the delivery of radionuclides to tumor tissues for decades. The
 creation of radioimmunoconjugates is predicated on the direct attachment
 of a radionuclide (i.e. a radiohalogen) or the initial
 bioconjugation of a chelator that can then be used to coordinate a
 radiometal. These two approaches have traditionally relied upon random
 reactions with residues within the immunoglobulin; however, stochastic
 modification strategies inevitably risk the integrity of the biomolecule
 itself and, as a result, can provide radioimmunoconjugates with subpar in
 vivo behavior. The initial evaluation of any novel radiolabeled antibody
 or antibody fragment should therefore involve the determination of the
 immunoconjugate's ability to bind its cognate antigen. These
 spreadsheets are companions to a manuscript in which we provide detailed
 protocols for three assays that can be used to determine the
 immunoreactivity of a radioimmunoconjugate: (1) a cell-based linear
 extrapolation assay; (2) a cell-based antigen saturation assay; and (3) a
 resin- or bead-based assay.  These are spreadsheets that
 accompany our guide to measuring the immunoreactivity of
 radioimmunoconjugates. 
description: **Summary of experimental efforts underlying this dataset** These are
 spreadsheets that accompany our guide to measuring the immunoreactivity of
 radioimmunoconjugates that appears in *Molecular Imaging and Biology*. The
 spreadsheets are currently filled with representative data collected
 according to the methods described in the *Supplementary Materials* that
 accompany the companion article in question. The spreadsheets are meant to
 be filled in with new raw data generated by readers of our protocols.
 **Description of file structure and contents** There are three Microsoft
 Excel spreadsheet files. The spreadsheets are currently filled with
 representative data collected according to the methods described in the
 *Supplementary Materials* that accompany the companion article in
 question. The spreadsheets are formatted in order to provide
 immunoreactivity values once users have filled them with data collected
 according to to the protocols described in the companion article in
 *Molecular Imaging and Biology.* **Definitions of all variables,
 abbreviations, missing data codes, and units** The following abbreviations
 are used. PBS = phosphate-buffered saline; CPM = counts-per-minute; Avg =
 Average; T/B = tube-to-background ratio; IR = immunoreactive fraction; %IR
 = percent immunoreactivity; std. deviation = standard deviation. There are
 no undefined variables, missing data codes, or missing units. **Links to
 other publicly accessible locations of the data** There are no links to
 other publicly accessible locations of the data. **Other sources, if any,
 that the data was derived from** There are no other sources of this data.
 **Any other details that may influence reuse or replication efforts**
 These issues are discussed at length in the companion article that is
 published in *Molecular Imaging and Biology*.