cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11391213
title: Period of latency, progression and duration of blockade of epidural anesthesia with lidocaine, bupivacaine or its association in dogs

description: ABSTRACT The objective of this study was to evaluate the latency, duration of the effect, and cranial progression of lidocaine and bupivacaine alone or in combination, by epidural route in dogs, and measuring the average pressure of the epidural channel before and after the completion thereof. Eighteen dogs were allocated in three groups, which received epidural: lidocaine 2% (GL) 0.25ml / kg; bupivacaine 0.5% (GB) in the same volume, or the association of both (GLB) in a 1: 1 ratio. Heart and respiratory rates and systolic blood pressure (SBP) were evaluated before treatment (M0) and up to 60 minutes after epidural anesthesia. In addition, the pressure in the epidural canal was evaluated before and after the administration of the treatments, latency period, progression and duration of the block by interdigital and paravertebral pannicus clamping. There was a 12% decrease in SBP in the GL at all times and 16% at 30 minutes in GLB when compared to the baseline. The mean pressure in the epidural space before and after epidural anesthesia was -1.5 (±3.9) and 41 (±16) mmHg), 55% presented negative pressure in the epidural space. The latency period did not differ between groups (GL: 3.5±1.6; GB: 4.5±4.5; and GLB: 2.4±1 minutes) and the duration of blockade was higher in GB (GL: 125±24, GB: 176±24, and GLB: 153±35 minutes). The maximum progression of anesthetics was up to L1-T13 in GL, L4-L3 in GB and L3-L2 in GLB. It is concluded that the association of lidocaine with bupivacaine does not present advantages in relation to the use of the drugs isolated by the epidural route, with lidocaine progressing more cranially in relation to bupivacaine or the association. Lidocaine promoted the reduction of SBP, even when associated with bupivacaine, remaining within the reference values. Only 55% of the dogs presented negative mean pressure in the epidural space before administration of the drugs, so the drop test may not be efficient for locating the epidural space in all animals.
DOI: 10.6084/m9.figshare.11391228.v1
title: Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification

description: ABSTRACT: This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. Firstly, embryos were disinfected, and after excision, plumules were pre-cultivated for 72 hours in Y3 + 0.6 M sucrose + 2.2 g L-1 Gelrite® culture medium. Plumules were exposed to PVS2 and PVS3 solutions for 15 and 30 minutes and rapidly immersed in liquid nitrogen (-196 ºC). After cryopreservation, they were thawed in culture medium solution (Y3 + 1.2 M sucrose) and cultured in regeneration medium. The experimental design was completely randomized in a 2x2 factorial scheme (vitrification solutions per exposure times), with five replicates per treatment. Data were compared by the Tukey’s test at 5% probability. Significant differences were observed in the callogenesis percentage for the solutions x exposure time interaction for non-cryopreserved cultures (-NL) and for exposure time after cryopreservation (+NL). PVS2 and PVS3 combined with 15 minutes of exposure promoted the highest callus formation (70 and 100%, respectively) in control cultures. The exposure time of 30 min, regardless of vitrification solution, resulted in 30% embryogenic callus formation after cryopreservation. These results contributed to the long-term conservation of coconut palm.
DOI: 10.6084/m9.figshare.11391204
title: Cross-cultural adaptation of the Preterm Parenting & Self-efficacy Checklist

description: ABSTRACT Objective: To conduct a cross-cultural adaptation of the Preterm Parenting & Self-Efficacy Checklist to the Brazilian Portuguese. Method: A methodological study was carried out with 51 parents of premature infants. Data analysis was based on psychometric and inferential statistical analyses. Results: The instrument content validation by experts obtained excellent agreement (97%) and the semantic analysis by the target population showed good understanding of the terms and ease of use. In test-retest, most participants were female (64.7%); and parents with higher education (47.1%). The instrument showed stability over time, with good internal consistency (α = 0.84). Four factors were generated in the subscales parenting self-efficacy, importance of tasks and self-perceived parental competence (67.0% to 74.2% of the variance). Conclusion: The properties of the Preterm Parenting & Self-Efficacy Checklist – Brazilian Version were maintained, which is a reliable indicator to evaluate the hospital discharge of premature infants.
DOI: 10.6084/m9.figshare.11391213.v1
title: Period of latency, progression and duration of blockade of epidural anesthesia with lidocaine, bupivacaine or its association in dogs

description: ABSTRACT The objective of this study was to evaluate the latency, duration of the effect, and cranial progression of lidocaine and bupivacaine alone or in combination, by epidural route in dogs, and measuring the average pressure of the epidural channel before and after the completion thereof. Eighteen dogs were allocated in three groups, which received epidural: lidocaine 2% (GL) 0.25ml / kg; bupivacaine 0.5% (GB) in the same volume, or the association of both (GLB) in a 1: 1 ratio. Heart and respiratory rates and systolic blood pressure (SBP) were evaluated before treatment (M0) and up to 60 minutes after epidural anesthesia. In addition, the pressure in the epidural canal was evaluated before and after the administration of the treatments, latency period, progression and duration of the block by interdigital and paravertebral pannicus clamping. There was a 12% decrease in SBP in the GL at all times and 16% at 30 minutes in GLB when compared to the baseline. The mean pressure in the epidural space before and after epidural anesthesia was -1.5 (±3.9) and 41 (±16) mmHg), 55% presented negative pressure in the epidural space. The latency period did not differ between groups (GL: 3.5±1.6; GB: 4.5±4.5; and GLB: 2.4±1 minutes) and the duration of blockade was higher in GB (GL: 125±24, GB: 176±24, and GLB: 153±35 minutes). The maximum progression of anesthetics was up to L1-T13 in GL, L4-L3 in GB and L3-L2 in GLB. It is concluded that the association of lidocaine with bupivacaine does not present advantages in relation to the use of the drugs isolated by the epidural route, with lidocaine progressing more cranially in relation to bupivacaine or the association. Lidocaine promoted the reduction of SBP, even when associated with bupivacaine, remaining within the reference values. Only 55% of the dogs presented negative mean pressure in the epidural space before administration of the drugs, so the drop test may not be efficient for locating the epidural space in all animals.
DOI: 10.6084/m9.figshare.11391216
title: Different times of application of equine chorionic gonadotropin in fixed-time artificial insemination protocols for dairy cows

description: ABSTRACT Different times of application of equine chorionic gonadotropin (eCG) in Fixed-Time Artificial Insemination (FTAI) protocols for dairy cows were evaluated. A total of 76 females were used, which received intravaginal progesterone devices on day zero (D0) of the protocol, which were withdrawn on D9, and the animals were then randomly distributed in three treatments: T1- application of eCG at the time of device withdrawal; T2 and T3 - application of eCG 48h and 24h before withdrawal of the devices, respectively. On D10 the animals received 1mg of GnRH and the FTAI was carried out 52 hours after the removal of the implant. There was no difference (P&gt;0.05) for interval from implant removal to ovulation (72.56±3.92h), diameter of the largest follicle of D9 (10.88±1.49mm), diameters of ovulatory follicle (15.15±1.16mm) and of the second largest follicle (7.49±0.52mm), follicle growth rate (1.38±0.04mm/day), ovulation rate (96.67%), interval between final diameter and initial diameter of dominant follicle (73.49±3.84h), corpus luteum area (2.27±0.43cm²), percentage of CL in right ovary (53.00%) and in left ovary (26.33%) and pregnancy rate (33.33%). The time of the application of eCG did not influence the efficiency of the protocol. We recommend the use of eCG at the time of the removal of the implant for management optimization.
DOI: 10.6084/m9.figshare.11391207
title: Alternative production of craft lager beers using artichoke (Cynara scolymus L.) as a hops substitute

description: Abstract Beer is a fermented beverage made from barley malt and hops under the action of a suitable yeast. The main function of hops in the brewing process is to provide bitterness, characteristic flavours and aromas to the beverage. However, hops only began to be added to the formulation of this beverage starting in the ninth century. Therefore, the use of other bitter plants as a substitute for hops may be a viable alternative. This study aimed at evaluating the use of artichoke as a total substitute for hops in American lager craft beer production. The substitution of hops with up to 2 g⋅L-1 artichoke resulted in a beer with a quality similar to that of the American lager beer traditionally found in the market. So, artichoke was confirmed to be a viable alternative for hops, having satisfactory physicochemical characteristics and good sensorial acceptance.
DOI: 10.6084/m9.figshare.11391216.v1
title: Different times of application of equine chorionic gonadotropin in fixed-time artificial insemination protocols for dairy cows

description: ABSTRACT Different times of application of equine chorionic gonadotropin (eCG) in Fixed-Time Artificial Insemination (FTAI) protocols for dairy cows were evaluated. A total of 76 females were used, which received intravaginal progesterone devices on day zero (D0) of the protocol, which were withdrawn on D9, and the animals were then randomly distributed in three treatments: T1- application of eCG at the time of device withdrawal; T2 and T3 - application of eCG 48h and 24h before withdrawal of the devices, respectively. On D10 the animals received 1mg of GnRH and the FTAI was carried out 52 hours after the removal of the implant. There was no difference (P&gt;0.05) for interval from implant removal to ovulation (72.56±3.92h), diameter of the largest follicle of D9 (10.88±1.49mm), diameters of ovulatory follicle (15.15±1.16mm) and of the second largest follicle (7.49±0.52mm), follicle growth rate (1.38±0.04mm/day), ovulation rate (96.67%), interval between final diameter and initial diameter of dominant follicle (73.49±3.84h), corpus luteum area (2.27±0.43cm²), percentage of CL in right ovary (53.00%) and in left ovary (26.33%) and pregnancy rate (33.33%). The time of the application of eCG did not influence the efficiency of the protocol. We recommend the use of eCG at the time of the removal of the implant for management optimization.
DOI: 10.6084/m9.figshare.11391198
title: Collagen production from chicken keel bone using acid and enzymatic treatment at a temperature of 30 °C

description: Abstract The aim of this study was to investigate the effect of acid pretreatment, temperature and enzyme concentration on the acid-enzymatic extraction of soluble collagen from chicken keel bone cartilage. A chemical composition analysis and protein profile characterization of this slaughter by-product were also conducted. The cartilages were extracted with 0.5 mol/L of acetic, lactic and citric acids for 24, 48 and 72 hours. Subsequently, optimization with a 22 factorial design was performed with three replications at the central point for a total of 7 experiments; the analyzed response was the collagen content of the obtained isolates. The cartilage under study has a high protein content (90.27% dry basis) consisting of approximately 35.7% collagen and is a source of essential and predominantly hydrophobic amino acids. Pretreatment with acetic acid for 24 hours led to greater extraction potential and consequently, a better collagen yield (30.12%). The electrophoretic profile of the obtained collagens revealed the existence of an α1 chain, indicating that this collagen was type II. Statistical analysis demonstrated that the acid-enzymatic procedure favored a temperature of 30 °C.
DOI: 10.6084/m9.figshare.11391204.v1
title: Cross-cultural adaptation of the Preterm Parenting & Self-efficacy Checklist

description: ABSTRACT Objective: To conduct a cross-cultural adaptation of the Preterm Parenting & Self-Efficacy Checklist to the Brazilian Portuguese. Method: A methodological study was carried out with 51 parents of premature infants. Data analysis was based on psychometric and inferential statistical analyses. Results: The instrument content validation by experts obtained excellent agreement (97%) and the semantic analysis by the target population showed good understanding of the terms and ease of use. In test-retest, most participants were female (64.7%); and parents with higher education (47.1%). The instrument showed stability over time, with good internal consistency (α = 0.84). Four factors were generated in the subscales parenting self-efficacy, importance of tasks and self-perceived parental competence (67.0% to 74.2% of the variance). Conclusion: The properties of the Preterm Parenting & Self-Efficacy Checklist – Brazilian Version were maintained, which is a reliable indicator to evaluate the hospital discharge of premature infants.
DOI: 10.6084/m9.figshare.11391207.v1
title: Alternative production of craft lager beers using artichoke (Cynara scolymus L.) as a hops substitute

description: Abstract Beer is a fermented beverage made from barley malt and hops under the action of a suitable yeast. The main function of hops in the brewing process is to provide bitterness, characteristic flavours and aromas to the beverage. However, hops only began to be added to the formulation of this beverage starting in the ninth century. Therefore, the use of other bitter plants as a substitute for hops may be a viable alternative. This study aimed at evaluating the use of artichoke as a total substitute for hops in American lager craft beer production. The substitution of hops with up to 2 g⋅L-1 artichoke resulted in a beer with a quality similar to that of the American lager beer traditionally found in the market. So, artichoke was confirmed to be a viable alternative for hops, having satisfactory physicochemical characteristics and good sensorial acceptance.
DOI: 10.6084/m9.figshare.11391192
title: Development of an optimized febuxostat self-nanoemulsified loaded transdermal film: <i>in-vitro, ex-vivo</i> and <i>in-vivo</i> evaluation

description: Febuxostat (FBX) is used to treat gout and chronic hyperuricemia. However, its bioavailability is moderate (49%) as a result of low solubility and first-pass metabolism. Therefore, the aim of our study is to improve FBX bioavailability by enhancement its solubility using self-nanoemulsifying drug delivery system (SNEDDS) technique in the form of transdermal film to avoid hepatic metabolism. To accomplish this goal, Eight SNEDDS formulae were prepared according to a three-factor, two-level D-Optimal mixture design to evaluate the effect of different ratios of the Lemon oil (X1), the surfactant Tween-20 (X2), and the co-surfactant PEG-400 (X3) on the globule size in order to reach smallest globular size. Results revealed that SNEDDS globule size ranged from 177 to 454 nm. The optimized formula consisted of 20% oil, 40% surfactant and 40% co-surfactant. Diffusion study showed improved enhancement in skin permeation that was confirmed by imaging using fluorescence microscope. <i>In vivo</i> plasma data showed significant (<i>p</i>
DOI: 10.6084/m9.figshare.11391198.v1
title: Collagen production from chicken keel bone using acid and enzymatic treatment at a temperature of 30 °C

description: Abstract The aim of this study was to investigate the effect of acid pretreatment, temperature and enzyme concentration on the acid-enzymatic extraction of soluble collagen from chicken keel bone cartilage. A chemical composition analysis and protein profile characterization of this slaughter by-product were also conducted. The cartilages were extracted with 0.5 mol/L of acetic, lactic and citric acids for 24, 48 and 72 hours. Subsequently, optimization with a 22 factorial design was performed with three replications at the central point for a total of 7 experiments; the analyzed response was the collagen content of the obtained isolates. The cartilage under study has a high protein content (90.27% dry basis) consisting of approximately 35.7% collagen and is a source of essential and predominantly hydrophobic amino acids. Pretreatment with acetic acid for 24 hours led to greater extraction potential and consequently, a better collagen yield (30.12%). The electrophoretic profile of the obtained collagens revealed the existence of an α1 chain, indicating that this collagen was type II. Statistical analysis demonstrated that the acid-enzymatic procedure favored a temperature of 30 °C.
DOI: 10.6084/m9.figshare.11391189
title: Effect of transcranial direct current stimulation on supramaximal intermittent exercise performance

description: Abstract Aim: Our purpose was to determine whether Transcranial Direct Current Stimulation (tDCS) improves performance in untrained individuals for supramaximal intermittent exercise. Methods: In a cross-over design, 11 healthy male subjects (26.8 ± 4.6 years) performed four Wingate trials after 20 minutes of anodal or sham tDCS over the left Insular Cortex (IC). For performance indexes, Relative Peak Power (RPP), Relative Average Power (RAP) and Fatigue Index (FI) were computed. Also, a Rating of Perceived Exertion (RPE) and Electromyography (EMG) signal were used to assess central and muscle fatigue development. Results: There was a significant difference over trials on all performance indexes, but there were no significant condition x trial interactions for any of the indexes. RPE increased significantly over trials, but there was no condition x trial interaction. There was no significant difference over trials on EMG for the rectus femoris and vastus medialis muscles; however, EMG decreased over trials for the vastus lateralis muscle. Furthermore, there was no condition x trial interaction on the EMG signal for any of the muscles. Conclusion: Our findings suggest that the anodal tDCS technique has no impact on physical performance, perceived exertion nor muscle fatigue development for supramaximal intermittent exercise.
DOI: 10.6084/m9.figshare.11391192.v1
title: Development of an optimized febuxostat self-nanoemulsified loaded transdermal film: <i>in-vitro, ex-vivo</i> and <i>in-vivo</i> evaluation

description: Febuxostat (FBX) is used to treat gout and chronic hyperuricemia. However, its bioavailability is moderate (49%) as a result of low solubility and first-pass metabolism. Therefore, the aim of our study is to improve FBX bioavailability by enhancement its solubility using self-nanoemulsifying drug delivery system (SNEDDS) technique in the form of transdermal film to avoid hepatic metabolism. To accomplish this goal, Eight SNEDDS formulae were prepared according to a three-factor, two-level D-Optimal mixture design to evaluate the effect of different ratios of the Lemon oil (X1), the surfactant Tween-20 (X2), and the co-surfactant PEG-400 (X3) on the globule size in order to reach smallest globular size. Results revealed that SNEDDS globule size ranged from 177 to 454 nm. The optimized formula consisted of 20% oil, 40% surfactant and 40% co-surfactant. Diffusion study showed improved enhancement in skin permeation that was confirmed by imaging using fluorescence microscope. <i>In vivo</i> plasma data showed significant (<i>p</i>
DOI: 10.6084/m9.figshare.11391195
title: Thyme essential oil for sheep: effect on rumen fermentation, nutrient digestibility, nitrogen metabolism, and growth

description: ABSTRACT Two experiments were conducted to evaluate the effect of thyme (Thymus vulgaris) essential oil (EO) doses on rumen fermentation, nutrient digestibility, and nitrogen metabolism, as well as performance and coccidia oocyst discharge. In experiment I, 20 rumen-cannulated wethers received the experimental diets containing 80% dry matter (DM) of haylage and 20% DM of concentrate. Treatments were 25mg of monensin/kg DM or doses of 1.25, 2.50, or 3.75g of thyme EO/kg DM. In experiment II, 50 ewe lambs received the same diets from experiment I, including a diet without feed additives. Wethers fed with diets containing 1.25g/kg DM of thyme EO had higher molar proportion of propionate (P= 0.03) and butyrate (P&lt; 0.01), and lower (P= 0.04) acetate to propionate ratio than other treatments. Adding thyme EO to diets increased (P= 0.02) nitrogen retention compared to monensin. The performance of ewe lambs was not affected (P≥ 0.05) by treatments. However, lambs fed monensin had a lower (P= 0.04) number of coccidia oocyst discharge than others. Adding 1.25g/kg DM of thyme EO in high-forage diet improved ruminal fermentation. Thyme EO enhanced nitrogen metabolism, however, it did not improve performance.
DOI: 10.6084/m9.figshare.11391180
title: Verbal instruction does not change myoelectric activity during seated row exercise in trained and untrained men

description: Abstract Aims: This study aimed to analyze the effect of verbal instruction on myoelectric activity (EMG) of the elbow flexors and shoulder extensors muscles during seated row exercise at 70%1RM, in trained and untrained individuals. Methods: The study sample comprised of seven males (21 ± 1.9 years) who had not practiced resistance training for at least one year (untrained group), and eight males (23 ± 2.6 years) who had regularly practice strength training for 2.4 ± 0.7 years (trained group). All individuals performed the seated row exercise with 70%1RM in conditions with and without verbal instruction. The EMG of the latissimus dorsi, teres major, posterior deltoid, triceps brachii long head and biceps brachii were measured. Results: There was no effect of verbal instruction on EMG at any muscle analyzed, for both groups. There was a difference between groups only for the teres major and triceps brachii (p &lt; 0.05). Untrained individuals had greater myoelectric activity in teres major and triceps brachii long head muscles compared to the trained group during the exercise.
DOI: 10.6084/m9.figshare.11391186
title: Perceptions and expectations of pregnant women about the type of birth

description: ABSTRACT Objective: to understand the perceptions and expectations of pregnant women about the type of birth. Method: this is a qualitative research, based on the assumptions of the Humanization of Obstetric Care. 15 pregnant women were interviewed twice at the beginning and the end of gestation between October 2015 and May 2016. Data were analyzed according to the method of the Discourse of the Collective Subject. Results: the discourses were associated with four themes: Advantages of vaginal birth over cesarean section; Fear and unpredictability of vaginal birth; Importance of the doctor in the definition of the type of birth; and Influence of family and friends in choosing the type of birth. Final considerations: according to pregnant women, vaginal birth has more benefit compared to cesarean section. However, during gestation and birth, fear of pain and the unexpected, and medical opinions of friends and family against vaginal birth strongly influence the choice of cesarean section.