DOI: 10.22002/mnftr-9n488
title: NR1769pKM101conjFM433

description: skinny ecoli conjugation with FM433 ecoli
description: <strong>Tilt Series Date:</strong>  2014-10-25</p>
 
<strong>Data Taken By:</strong>  Elitza Tocheva</p>
 
<strong>Species / Specimen:</strong>  Escherichia coli</p>
 
<strong>Strain:</strong>  NR1769 pKM101</p>
 
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å², defocus: -10.0 μm, magnification: 18000x. </p>
 
<strong>Microscope:</strong>  Caltech Polara</p>
 
<strong>Acquisition Software:</strong>  UCSFTomo</p>
 
<strong>Upload Method:</strong>  reupload_downloaded</p>
 
<strong>Processing Software Used:</strong>  IMOD</p>
 
<strong>Collaborators and Roles: </strong> Christian Baron</p>
 
<strong>Purification / Growth Conditions / Treatment:</strong>  cells were grown on a gold grid in a drop of 1:1 mix of bacteria for 30min</p>
 
<strong>Sample Preparation:</strong>  Vitrobot</p>
 
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/rawdata/</p>
 
NRpKMplusFM433_conj1_18K0002.mrc, Tilt Series (Pixel Size 0.63 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/rawdata/NRpKMplusFM433_conj1_18K0002.mrc" > <i class="download icon"></i> Download </a>
</p>
 
 NRpKMplusFM433_conj1_18K0002_full.rec, Reconstruction (Pixel Size 0.0 nm), 980.0 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/3dimage_49693/NRpKMplusFM433_conj1_18K0002_full.rec" > <i class="download icon"></i> Download </a>
</p>
 
 slicer000.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12463/slicer000.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer001.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12464/slicer001.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer002.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12465/slicer002.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer003.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12466/slicer003.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer004.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12467/slicer004.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer005.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12468/slicer005.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer006.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12469/slicer006.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer007.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12470/slicer007.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer008.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12471/slicer008.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer009.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12472/slicer009.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer010.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12473/slicer010.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer011.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12474/slicer011.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer012.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12475/slicer012.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer013.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12476/slicer013.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer014.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12477/slicer014.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer015.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12478/slicer015.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer016.tif, 2.3 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-7/file_12479/slicer016.tif" > <i class="download icon"></i> Download </a>
</p>