DOI: 10.5061/dryad.m37pvmdcw
title: Data for: Pro-Inflammatory response to macrotextured silicone implant wear debris

description: Macrotextured silicone breast implants are associated with several
 complications, ranging from seromas and hematomas to the formation of a
 rare type of lymphoma, known as breast implant-associated anaplastic large
 cell lymphoma (BIA-ALCL). The presence of silicone wear debris has been
 detected within the peri-implant region and fibrotic capsule and
 histological analyses reveal inflammatory cells surrounding debris
 particles. However, it is unclear how these debris particles are generated
 and released from macrotextured implant surfaces, and whether wear debris
 generation is related to implant stiffness. In this study, we created an
 accelerated implant aging model to investigate the formation of silicone
 wear debris produced from self-mated (“shell-shell”) tribological
 interactions. We created implant-like silicone elastomers from
 polydimethylsiloxane (PDMS) using Sylgard 184 base:curing agent (10:1,
 12:1, and 16:1) and quantified their mechanical properties
 (E* = 1141 ± 472, 336 ± 20, and 167 ± 53 kPa, respectively). We created
 macrotextured PDMS samples using the lost-salt technique and compared
 their self-mated friction coefficient (&lt; µ &gt;  = 4.8 ± 3.2,
 4.9 ± 1.8, and 6.0 ± 2.3, respectively) and frictional shear stress
 (τ = 3.1 ± 1.3, 3.2 ± 1.7, and 2.4 ± 1.4 MPa, respectively) to those of
 the recalled Allergan Biocell macrotextured implant shell (E* = 299 ± 8
 kPa, &lt; µ &gt;  = 2.2, and τ = 0.8 ± 0.1). Friction coefficient
 and frictional shear stress were largely insensitive to variations in
 elastic modulus for macrotextured PDMS samples and recalled implant
 shells. The stiffest 10:1 PDMS macrotextured sample and the recalled
 implant shell both generated similar area fractions of silicone wear
 debris.
description: # Data for: Pro-Inflammatory response to macrotextured silicone implant
 wear debris
 [https://doi.org/10.5061/dryad.m37pvmdcw](https://doi.org/10.5061/dryad.m37pvmdcw) Atkins, D.J., Rogers, A.E., Shaffer, K.E. *et al.* Pro-Inflammatory Response to Macrotextured Silicone Implant Wear Debris. *Tribol Lett* **73**, 30 (2025). [https://doi.org/10.1007/s11249-025-01965-6]() Corresponding author: Angela Pitenis, [apitenis@ucsb.edu](mailto:apitenis@ucsb.edu) ## Description of the data and file structure ### Files and variables The compressed folder **2025_Atkins_TribLett_DataDryad.zip** contains images and the data to reproduce the figures from the associated manuscript. A) Figure2a_representative_indentation_curves.csv \* row 1: labels for column data with units \* row 2 and beyond: data \* column 1: implant sample z-stage position, in micrometers, the x-axis of the force-indentation plot \* column 2: implant sample normal force, Fn, in micro-Newtons, the y-axis of the force-indentation plot \* column 3: 10:1 PDMS sample z-stage position, in micrometers, the x-axis of the force-indentation plot \* column 4: 10:1 PDMS sample normal force, Fn, in micro-Newtons, the y-axis of the force-indentation plot \* column 5: 12:1 PDMS sample z-stage position, in micrometers, the x-axis of the force-indentation plot \* column 6: 12:1 PDMS sample normal force, Fn, in micro-Newtons, the y-axis of the force-indentation plot \* column 7: 16:1 PDMS sample z-stage position, in micrometers, the x-axis of the force-indentation plot \* column 8: 16:1 PDMS sample normal force, Fn, in micro-Newtons, the y-axis of the force-indentation plot B) Figure2b_contact_area_measurements.csv \* row 1: labels for column data showing which sample type and normal load were used \* row 2 and beyond: data of contact area \* column N: labels for average and standard deviation calculations \* column M: labels showing all units are in micrometers squared \* column A: 10:1 PDMS sample contact area with 1 mN of load, in micrometers squared \* column B: 10:1 PDMS sample contact area with 3 mN of load, in micrometers squared \* column C: 10:1 PDMS sample contact area with 5 mN of load, in micrometers squared \* column D: 12:1 PDMS sample contact area with 1 mN of load, in micrometers squared \* column E: 12:1 PDMS sample contact area with 3 mN of load, in micrometers squared \* column F: 12:1 PDMS sample contact area with 5 mN of load, in micrometers squared \* column G: 16:1 PDMS sample contact area with 1 mN of load, in micrometers squared \* column H: 16:1 PDMS sample contact area with 3 mN of load, in micrometers squared \* column I: 16:1 PDMS sample contact area with 5 mN of load, in micrometers squared \* column J: implant sample contact area with 1 mN of load, in micrometers squared \* column K: implant sample contact area with 3 mN of load, in micrometers squared \* column L: implant sample contact area with 5 mN of load, in micrometers squared C) Figure2b_raw_image_data.zip \* raw image data that was used to generate contact area measurements for file B) Figure2_contact_area_measurements.csv \* raw confocal image data used to generate Figure S3, where fluorescent intensity represents contact between the glass substrate and textured PDMS probe \## Figure 3: friction measurements D) Figure3_16to1_friction_loop.csv \* row 1: labels for column data with units \* column A: time, in seconds, of indentation \* column B: normal force, Fn, in micro-Newtons, or the y-axis of Figure 3b \* column C: standard deviation in the normal force, Fn, in micro-Newtons \* column D: friction force, Ff, in micro-Newtons, or the y-axis of Figure 3c \* column E: standard deviation in the friction force, Ff, in micro-Newtons \* column F: friction coefficient, mu \* column G: standard deviation in the friction coefficient, mu \* column H: x-stage position in micrometers, or the x-axis of Figures 3b and 3c \## Figure 4, Figure S4, and Figure S5: friction coefficient and shear stress measurements E) Figure4a_16to1_1000mm_friction_coefficient.csv \* row 1: labels for column data \* row 2: unit labels for column data \* column 1: friction force average, Ff, in micro-Newtons \* column 2: normal force average, Fn, in micro-Newtons \* column 3: average friction coefficient, mu \* column 4: sliding distance, in mm F) Figure4b_average_friction_coefficient_bar_plot.csv \* row 1: labels for sample type (10:1 PDMS, 12:1 PDMS, 16:1 PDMS, and implant sample) \* row 2: labels showing that each of these values are friction coefficient values \* row 3: labels for which columns are average values, and which columns are standard deviation values \* row 4: data on friction coefficient average and standard deviation \* column 1: 10:1 PDMS friction coefficient average value \* column 2: 10:1 PDMS friction coefficient standard deviation \* column 3: 12:1 PDMS friction coefficient average value \* column 4: 12:1 PDMS friction coefficient standard deviation \* column 5: 16:1 PDMS friction coefficient average value \* column 6: 16:1 PDMS friction coefficient standard deviation \* column 7: implant sample friction coefficient average value G) Figure4c_shear_stress_bar_plot.csv \* row 1: column labels \* column 1: row labels for sample type \* column 2: shear stress data, in MegaPascals \* column 3: standard deviation in the shear stress, in MegaPascals S) FigureS4a_10to1_friction_vs_sliding_distance.csv \* row 1: column labels \* row 2 and beyond: data \* column 1: sliding distance, in millimeters \* column 2: average friction coefficient, mu T) FigureS4b_12to1_friction_vs_sliding_distance.csv \* row 1: column labels \* row 2 and beyond: data \* column 1: sliding distance, in millimeters \* column 2: average friction coefficient, mu U) FigureS5_average_friction_coefficient_scatter_plot.csv \* row 1: column labels with units (column 1 units are millimeters, and all other columns are unitless) \* row 2 and beyond: data \* column 1: sliding distance in millimeters \* column 2: average friction coefficient of the 10:1 PDMS sample \* column 3: standard deviation in the friction coefficient of the 10:1 PDMS sample \* column 4: average friction coefficient of the 12:1 PDMS sample \* column 5: standard deviation in the friction coefficient of the 12:1 PDMS sample \* column 6: average friction coefficient of the 16:1 PDMS sample \* column 7: standard deviation in the friction coefficient of the 16:1 PDMS sample \## Figure 5 and Figure S6: debris particles H) Figure5a&b_debris_particle_size.csv \* row 1: column labels, with units (micrometers squared) \* row 2 and beyond: data \* column 1: 10:1 PDMS debris particle sizes, in micrometers squared \* column 2: 12:1 PDMS debris particle sizes, in micrometers squared \* column 3: 16:1 PDMS debris particle sizes, in micrometers squared \* column 4: implant sample debris particle sizes, in micrometers squared I) Figure5d_10to1_PDMS_particle.tif \* raw TIF of 10:1 PDMS particle debris acquired using SEM J) Figure5e_12to1_PDMS_particle.tif \* raw TIF of 12:1 PDMS particle debris acquired using SEM K) Figure5f_16to1_PDMS_particle.tif \* raw TIF of 16:1 PDMS particle debris acquired using SEM L) Figure5g_implant_particle.tif \* raw TIF of implant sample particle debris acquired using SEM V) FigureS6_debris_particle_images.zip \* this folder contains three raw confocal files in .nd2 format showing silicone debris particles \* 10to1_debris_particles.nd2 shows debris particles for the 10:1 PDMS sample \* 12to1_debris_particles.nd2 shows debris particles for the 12:1 PDMS sample \* 16to1_debris_particles.nd2 shows debris particles for the 16:1 PDMS sample \* each .nd2 file contains 18 fields of view of 370x370 micrometers in area and represents comparable quantities of debris generation among each (10:1, 12:1, 16:1) PDMS sample during self-mated friction measurements \## Figure 6 and Figure S10: macrophage imaging and cytokine secretion M) Figure6a&b_macrophage_image.tif \* confocal image shown in Figures 6a and b; green intensity shows the presence of silicone debris particles, and magenta intensity shows cell bodies N) Figure6c&d_area_fraction_IL6_TNF_intensity.csv \* row 1: column labels describing sample type area fraction of debris particles, fluorescence intensity of IL-6, and fluorescence intensity of TNF-alpha \* row 2 and beyond: data \* column A: 10:1 PDMS sample area fractions measured \* column B: 10:1 PDMS sample IL-6 fluorescence intensity measured compared to the area fraction \* column C: 10:1 PDMS sample TNF-alpha fluorescence intensity measured compared to the area fraction \* column D: 12:1 PDMS sample area fractions measured \* column E: 12:1 PDMS sample IL-6 fluorescence intensity measured compared to the area fraction \* column F: 12:1 PDMS sample TNF-alpha fluorescence intensity measured compared to the area fraction \* column G: 16:1 PDMS sample area fractions measured \* column H: 16:1 PDMS sample IL-6 fluorescence intensity measured compared to the area fraction \* column I: 16:1 PDMS sample TNF-alpha fluorescence intensity measured compared to the area fraction \## Figure S1: SEM micrographs O) FigureS1a_micrograph.tif \* scanning electron microscopy micrograph of silicone implant cross section P) FigureS1b_micrograph.tif \* scanning electron microscopy micrograph of textured silicone cross section \## Figure S2: OPTIR infrared spectroscopy Q) FigureS2_OPTIR_implant_smooth_side.csv \* raw data file used to plot IR spectra for the implant sample in Figure S2a R) FigureS2_OPTIR_12to1PDMS_smooth_side.csv \* raw data file used to plot IR spectra for the 12:1 PDMS sample in Figure S2c \## Figure S7: binucleated cells W) FigureS7_binucleated_cells_box_plot.csv \* column 1: row labels showing sample type \* column 2 and beyond: data \* row 1: % binucleated cells for negative control, PBS \* row 2: % binculeated cells for positive control, LPS \* row 3: % binucleated cells for a 10:1 PDMS debris sample \## Figure S8: small polyacrylamide particles X) FigureS8a.jpeg \* maximum intensity projection showing small (1-10 micrometers) polyacrylamide particles that were added to culture with macrophages Y) FigureS8b.jpeg \* maximum intensity projection showing medium (15-25 micrometers) polyacrylamide particles that were added to culture with macrophages \## Figure S9: small polyacrylamide particle cytokine analysis Z) FigureS9_polyacrylamide_particles_cytokine_panel.csv \* column 1: row labels showing sample type \* column 2: data for IL-6 secretion in fluorescence intensity units \* column 3: data for MCP-1 secretion in fluorescence intensity units \* column 4: data for TNF-alpha secretion in fluorescence intensity units