DOI: 10.5061/dryad.k3j9kd5gv
title: Data from: Dynamic effects of thermal acclimation on chytridiomycosis infection intensity and transmission potential in Xenopus laevis

description: The pandemic amphibian pathogen Batrachochytrium dendrobatidis (Bd) can
 cause more severe infections with variable temperatures due to delays in
 host thermal acclimation following temperature shifts. However, little is
 known about the timing of these acclimation effects or their consequences
 for Bd transmission. We measured how thermal acclimation affects Bd
 infection in Xenopus laevis, using a timing-of-exposure treatment to
 investigate acclimation effect persistence following a temperature shift.
 Consistent with a delay in host acclimation, warm-acclimated frogs exposed
 to Bd immediately following a temperature decrease (Day 0) developed
 higher infection intensities than frogs already acclimated to the cool
 temperature. This acclimation effect was surprisingly persistent (5
 weeks). Acclimation did not affect infection intensity when Bd exposure
 occurred one week after the temperature shift, indicating that frogs fully
 acclimated to new temperatures within 7 days. This suggests that
 acclimation effect persistence beyond one week post-exposure was caused by
 carry-over from initially high infection loads, rather than an extended
 delay in host acclimation. In a second experiment, we replicated the
 persistent thermal acclimation effects on Bd infection but found no
 acclimation effects on zoospore production. This suggests that variable
 temperatures consistently exacerbate individual Bd infection but may not
 necessarily increase Bd transmission.
description: # Noelker\_etal\_XenopusBdAcclimation This study investigates the effects
 of acclimation and performance temperatures on *Xenopus laevis* Bd
 infections from skin swabs and filters measuring zoospore production.
 **TITLE:** *Dynamic effects of thermal acclimation on chytridiomycosis
 infection intensity and transmission potential in Xenopus laevis* James E.
 Noelker (Corresponding author:
 [jaynoelker@gmail.com](mailto:jaynoelker@gmail.com)) Vitoria Abreu Ruozzi
 Kyle D. Spengler Hunter M. Craig Thomas R. Raffel (Principle investigator)
 \=========================================================================== **File 1:** *Experiment1&2TemperatureData.csv* CSV of temperature data from Experiment 1 & 2 comparing target temperatures with data collected using HOBO temperature loggers in each incubator. | Variable name | Description | | -------------- | ------------------------------------------------------------------------------------------------------------- | | **Experiment** | unique identifier for each experiment as either 2021.IndInf for Experiment 1 and 2022.IndInf for Experiment 2 | | **IncNum** | each incubator's identification number | | **Block** | spatial block for each incubator form Experiment 1 or Experiment 2 | | **Treatment** | assigned target temperature treatment for each incubator in Celcius | | **AvgTempC** | average measured temperature for each incubator in Celcius | | **SDTempC** | standard deviation for each incubator in Celcius | \=========================================================================== **File 2:** *Experiment1&2CombinedBdInfectionData.csv* CSV of Bd infection data collected from skin swabs for Experiments 1 & 2 collected weekly and filters for Experiment 2 collected on day 7 and 35. Each row is a data collection day for each frog as well as the conditions the frog was experiencing. Our data includes cells with values of "NA" (not applicable) meaning no data was collected for that variable. However, the cells are kept in the file which is essential for accurate analysis of the dataset using our R script. | Variable name | Description | | --------------------- | --------------------------------------------------------------------------------------------------------------------- | | **Experiment** | unique identifier for each experiment as either 2021.IndInf for Experiment 1 and 2022.IndInf for Experiment 2 | | **Year** | year the experiment was conducted | | **SampleID** | unique identifier for each swab sample taken from a frog on a particular day (**FrogID\_Date**) | | **FrogID** | unique frog identification number for each experiment | | **Block** | spatial block for within each experiment | | **Exp.Block** | spatial block for each experiment (**Year.Block**) | | **Date** | actual date a swab or filter sample was collected | | **Day** | day of the experiment relative to the temperature shift | | **DayOfExpo** | each frog's day of exposure to Bd zoospores relative to the temperature shift | | **TimeSinceExposure** | number of days since exposure for a frog for a swab or filter sample | | **AccTemp** | a frog's assigned acclimation temperature | | **PerfTemp** | a frog's assigned performance temperature | | **AccInc** | a frog's assigned acclimation incubator | | **PerfInc** | a frog's assigned performance incubator | | **LnSwabBd** | log(Bd zoospore equivelents) after qPCR assay from frog swabs | | **LnFilterBd** | log(Bd zoospore equivelents) after qPCR assay from frog filters | | **TotalTime.min** | the total length of time in minutes that a frog was "soaked" in clean water during the zoospore production experiment | | **TotalVolume.mL** | the total volume of water in mL filtered after a frog "soaked" | | **BdStatus** | A frog’s infection status as either infected (1) or uninfectied (0) | | **Notes** | any notes collected about a given sample | \=========================================================================== **File 3:** *Experiment1&2CombinedMassData.csv* CSV of mass data for all frogs for Experiments 1 & 2. | Variable name | Description | | ---------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | **Experiment** | year the experiment was conducted | | **FrogCode** | unique identifier for each frog (**FrogID\_Block**) | | **InitialMass** | mass of each frog in grams for both experiments at the beginning of each experiment | | **DayOfInfectionMass** | mass of each frog in grams for both experiments on the day they were exposed to Bd | | **DeathMass** | mass of each frog in grams for both experiments on the day they died | | **DeathDate** | date that each frog died | | **CauseOfDeath** | the cause of death for each frog in both experiments as either **EndOfExperiment.Euthanized** if a frog was euthanized at the end of the experiment or **EndPoint.Euthanized** if a frog was euthanized during the experiment due to sever Bd infection | \===========================================================================