DOI: 10.5281/zenodo.14252610
title: Data from: Turfgrass pedogenesis under low maintenance: an experimental analysis with Festuca rubra subspecies at different fertilization levels

description: Fine-leaved turfgrass (sub)species can deal with stress situations resulting from climate change and a legislatory ban on chemical pest control, without losing turfgrass playability in golf. Low maintenance, including very low N-input and non-disturbance, is suggested to support turfgrass playability via control of excessive plant biomass growth to overcome soil layering. In a pot experiment, the development of visible plant biomass (VPB) in distinctive soil layers, thatch, mat and deeper soil layers, was analyzed in response to low N fertilization levels and non-disturbance maintenance, in three monocultures of fine-leaved Festuca rubra subspecies over the first 4 years of establishment. 

The experiment was designed as a factorial randomized block design with 4 replicates, consisting three N-fertilizer application levels (30, 60 and 90 kg N.ha-1.year-1), 3 Festuca rubra subspecies and three varieties per subspecies (Table 5.1). Varieties grew during 4 years in outdoor placed PVC pots (12.5 l, Ø 20 cm) constructed as a sandy USGA green with 30 cm rootzone over 10 cm gravel layer (USGA, 2015) at Lumbricus research facility in Herveld, The Netherlands (51°54’10.017”N, 5°44’10.586”E). The rootzone consisted a 95/5 v/v sand/heather compost mixture containing sand with a median grain size of 330 µm. For practical reasons pots were mown manually with a scissor, once a week at 6 mm height during growing season with clippings removed. Pots were only irrigated with tap water during dry warm periods when soil moisture content in the upper 6 cm dropped below 8% v/v,.

An indication of growth rate was obtained from the collection of turfgrass clippings per pot during 7 months of the growing period in 2019. Fresh weight was measured monthly by collecting 4 clipping rounds per pot and stored in a fridge at 4 °C until measurements on a compound sample per pot at the end of the month were taken. In each pot, cores of the top 20 cm of the soil including aboveground biomass were taken with a core sampler (diameter 28 mm) in May/June 2018, 2019, 2020 and 2021 and in October 2018 and 2019. Cores were immediately divided into 4 distinctive layers, thatch + aboveground biomass, mat, remaining upper 10 cm soil and 10-20 cm soil. Distinction of the 4 layers followed the protocol of Evers et al. (2024). Aboveground biomass was separated with a scissor from the thatch. Sediment from thatch, mat, remaining upper 10 cm of soil and 10-20 cm soil was then carefully washed out with tap water, after which the remaining below-ground (dead and living) visible plant biomass and aboveground biomass was dried at 65 °C until stable weight and weighed. Total C and N analyses were carried out, from which C and N concentrations (in % of dry matter of plant biomass) and C to N ratios (CN ratio) were calculated. Immediately after the last moment of measurements in June 2021, one cultivar per subspecies of all these three cultivars were selected for microbial analyses in thatch and mat layers in both the low and high N-fertilizer input treatments, in all 4 replicates. Profiling of microbial communities via phospholipid fatty acid (PLFA) analysis was carried out following the protocol of Buyer and Sasser (2012). The MIDI Sherlock PLFA Software Package (MIDI, Newark, Delaware, USA) was used for peak identification.

Per turfgrass subspecies or variety, the best fitted line for thatch and mat depth development, i.e., with the smallest rse, was iteratively calculated by linear least square regression with the lm function in R (version 4.1.3; 2022-03-10) and by non-linear least square regression with the nls function in R. For the fitting of the statistical models, either a linear function (F (χ) = αχ +β), a logistic function (F (χ) = α/(1+e-(βχ+γ)) or a hyperbolic function (F (χ) = αχ/(β + χ)), was used, based on the best fit for the respective turfgrass species or variety.Best fit curves for the thatch and mat depth were determined from RMSE with the RMSE function in the qpcR package of R giving the possibility to compare the best fit between linear and non-linear models, and p values after examination of various possible models. With SPSS the effect of subspecies and N-fertilizer over time on growth rate, visible plant biomass concentration and CN ratio in a distinctive soil layer was analyzed with general linear models with time as repeated measurement. Mauchly’s test of sphericity was used with p < 0.05 for choosing the correct test of within-subject effects. When sphericity was not assumed, statistical correction of chi-square was done conservatively with Greenhouse-Giesser method when epsilon < 0.75 and with the lower-bound method when epsilon > 0.75. Multiple comparison for means across groups was done with Bonferroni's post hoc test when equal variances between groups were assumed.
description: freshweight.csv

(Frc = Festuca rubra commutata, Frt = Festuca rubra trichophylla, Frr = Festuca rubra rubra)



spec = subspecies

var = variety

rep = replicate

Nfert = N fertilization (kg N ha-1)

t1 = Leaf biomass growth at 30-3-2019 (mg fresh weight.pot-1.day-1)

t2 = Leaf biomass growth at 28-4-2019 (mg fresh weight.pot-1.day-1)

t3 = Leaf biomass growth at 1-6-2019 (mg fresh weight.pot-1.day-1)

t4 = Leaf biomass growth at 29-6-2019 (mg fresh weight.pot-1.day-1)

t5 = Leaf biomass growth at 29-7-2019 (mg fresh weight.pot-1.day-1)

t6 = Leaf biomass growth at 31-8-2019 (mg fresh weight.pot-1.day-1)

t7 = Leaf biomass growth at 5-10-2019 (mg fresh weight.pot-1.day-1)
description: ThatchMatThickeness.csv

(Frc = Festuca rubra commutata, Frt = Festuca rubra trichophylla, Frr = Festuca rubra rubra)



spec = subspecies

var = variety

rep = replicate number

Nfert = N fertilization (kg N ha-1)

thatch1 = thatch thickness at 18-5-2018 (cm)

thatch2 = thatch thickness at 26-10-2018 (cm)

thatch3 = thatch thickness at 17-5-2019 (cm)

thatch4 =  thatch thickness at 29-10-2019 (cm)

thatch5 = thatch thickness at 10-6-2020 (cm)

thatch6 = thatch tcicknesss at 16-6-2021 (cm)

mat1 = mat thickness at 18-5-2018 (cm)

mat2 = mat thickness at 26-10-2018 (cm)

mat3 = mat thickness at 17-5-2019 (cm)

mat4 =  mat thickness at 29-10-2019 (cm)

mat5 = mat thickness at 10-6-2020 (cm)

mat6 = mat tcicknesss at 16-6-2021 (cm)
description: VPBlayerN.csv

(Frc = Festuca rubra commutata, Frt = Festuca rubra trichophylla, Frr = Festuca rubra rubra)



layer = soil layer (t = thatch, m = mat, s= remaining soil)

rep = replicate numvber

year = years after start

Frc30 = Visible plant biomass with Frc at 30 kg N ha-1 (mg dry matter cm-3)

Frt30 = Visible plant biomass with Frt at 30 kg N ha-1 (mg dry matter cm-3)

Frt30 = Visible plant biomass with Frr at 30 kg N ha-1 (mg dry matter cm-3)

Frc60 = Visible plant biomass with Frc at 60 kg N ha-1 (mg dry matter cm-3)

Frt60 = Visible plant biomass with Frt at 60 kg N ha-1 (mg dry matter cm-3)

Frt60 = Visible plant biomass with Frr at 60 kg N ha-1 (mg dry matter cm-3)

Frc90 = Visible plant biomass with Frc at 90 kg N ha-1 (mg dry matter cm-3)

Frt90 = Visible plant biomass with Frt at 90 kg N ha-1 (mg dry matter cm-3)

Frt90 = Visible plant biomass with Frr at 90 kg N ha-1 (mg dry matter cm-3)
description: CNratio.csv

(Frc = Festuca rubra commutata, Frt = Festuca rubra trichophylla, Frr = Festuca rubra rubra)



spec = subspecies

var = variety

rep = replicate number

Thatch1 = CN ratio in Visible plant biomass in thatch at 18-5-2018

Thatch2 = CN ratio in Visible plant biomass in thatch at 17-5-2019

Thatch3 = CN ratio in Visible plant biomass in thatch at 10-6-2020

Thatch4 = CN ratio in Visible plant biomass in thatch at 21-6-2021

Mat1 = CN ratio in Visible plant biomass in mat at 18-5-2018

Mat2 = CN ratio in Visible plant biomass in mat at 17-5-2019

Mat3 = CN ratio in Visible plant biomass in mat at 10-6-2020

Mat4 = CN ratio in Visible plant biomass in mat at 21-6-2021
description: Microbes.csv

(Frc = Festuca rubra commutata, Frt = Festuca rubra trichophylla, Frr = Festuca rubra rubra)



spec = subspecies

rep = replicate number

Nfert = N fetrtilization (kg N ha-1)

ThatchF = Fungi in thatch (% of total microbes in thatch)

ThatchB = Bacteria in thatch (% of total microbes in thatch)

ThatchA = Actinomycetes in thatch (% of total microbes in thatch)

MatF = Fungi in mat (% of total microbes in thatch)

MatB = Bacteria in mat (% of total microbes in thatch)

MatA = Actinomycetes in mat (% of total microbes in thatch)

Thatchtot = Total microbes in thatch (picamol g soil-1)

Mattot = Total microbes in mat (picamol g soil-1)