cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21679065
title: Dose-dependent effects of neem crude extract on human dental pulp cell and murine osteoblast viability and mineralization

description: Abstract Neem has been used as a medicine due to its beneficial properties such as anti-microbial effects. Neem products for oral application are on the rise. Before recommendation for therapeutic use in human, its effects on cellular activities need to be examined. Therefore, the aim of this study was to test the effects of the ethanolic neem crude extract on dental pulp cells and osteoblasts in terms of cell viability, mineralization, and gene expressions. The ethanolic neem extract derived from dry neem leaves was subjected to chemical identification using GC-MS. Human dental pulp stem cells (hDPSCs) and pre-osteoblasts (MC3T3) were treated with various concentrations of the neem crude extract. Cell viability, mineralization, and gene expressions were investigated by MTT assay, real-time PCR, and alizarin red S assay, respectively. Statistical analysis was performed by one-way ANOVA followed by Dunnett test. GC-MS detected several substance groups such as sesquiterpene. Low to moderate doses of the neem crude extract (4 - 16 µg/ml) did not affect hDPSC and MC3T3 viability, while 62.5 µg/ml of the neem extract decreased MC3T3 viability. High doses of the neem crude extract (250 - 1,000 µg/ml) significantly reduced viability of both cells. The neem crude extract at 1,000 µg/ml also decreased viability of differentiated hDPSC and MC3T3 and their mineralization. Furthermore, 4 µg/ml of neem inhibited viability of differentiated hDPSC. There is no statistical difference in gene expressions related to cell differentiation. In conclusion, the neem crude extract affected cell viability and mineralization. Cell viability altered differently depending on the doses, cell types, and cell stages. The neem crude extract did not affect cell differentiation. Screening of its effect in various aspects should be examined before the application for human use.
DOI: 10.6084/m9.figshare.21679065.v1
title: Dose-dependent effects of neem crude extract on human dental pulp cell and murine osteoblast viability and mineralization

description: Abstract Neem has been used as a medicine due to its beneficial properties such as anti-microbial effects. Neem products for oral application are on the rise. Before recommendation for therapeutic use in human, its effects on cellular activities need to be examined. Therefore, the aim of this study was to test the effects of the ethanolic neem crude extract on dental pulp cells and osteoblasts in terms of cell viability, mineralization, and gene expressions. The ethanolic neem extract derived from dry neem leaves was subjected to chemical identification using GC-MS. Human dental pulp stem cells (hDPSCs) and pre-osteoblasts (MC3T3) were treated with various concentrations of the neem crude extract. Cell viability, mineralization, and gene expressions were investigated by MTT assay, real-time PCR, and alizarin red S assay, respectively. Statistical analysis was performed by one-way ANOVA followed by Dunnett test. GC-MS detected several substance groups such as sesquiterpene. Low to moderate doses of the neem crude extract (4 - 16 µg/ml) did not affect hDPSC and MC3T3 viability, while 62.5 µg/ml of the neem extract decreased MC3T3 viability. High doses of the neem crude extract (250 - 1,000 µg/ml) significantly reduced viability of both cells. The neem crude extract at 1,000 µg/ml also decreased viability of differentiated hDPSC and MC3T3 and their mineralization. Furthermore, 4 µg/ml of neem inhibited viability of differentiated hDPSC. There is no statistical difference in gene expressions related to cell differentiation. In conclusion, the neem crude extract affected cell viability and mineralization. Cell viability altered differently depending on the doses, cell types, and cell stages. The neem crude extract did not affect cell differentiation. Screening of its effect in various aspects should be examined before the application for human use.
DOI: 10.6084/m9.figshare.21679064
title: Knowledge-intensive business services in the metropolitan regions of São Paulo: a 2016-2020 panorama

description: Abstract In the context of innovation, special importance is given to Knowledge-Intensive Business Services (Kibs), which is a group of companies that offer solutions based on technical knowledge to other companies. The presence of these companies in the territory indicates sectoral diversity and economic dynamism; in addition, the services provided by Kibs are considered a fundamental part of the innovation process. From this perspective, the present work aims to identify the presence and evolution of the Kibs sector, from 2016 to 2020, in the nine metropolitan regions of the State of São Paulo. The study showed the differentiated dynamics of this group of companies present in the metropolitan regions analyzed here.
DOI: 10.6084/m9.figshare.21679064.v1
title: Knowledge-intensive business services in the metropolitan regions of São Paulo: a 2016-2020 panorama

description: Abstract In the context of innovation, special importance is given to Knowledge-Intensive Business Services (Kibs), which is a group of companies that offer solutions based on technical knowledge to other companies. The presence of these companies in the territory indicates sectoral diversity and economic dynamism; in addition, the services provided by Kibs are considered a fundamental part of the innovation process. From this perspective, the present work aims to identify the presence and evolution of the Kibs sector, from 2016 to 2020, in the nine metropolitan regions of the State of São Paulo. The study showed the differentiated dynamics of this group of companies present in the metropolitan regions analyzed here.
DOI: 10.6084/m9.figshare.21679068
title: Application of natural antioxidants in animal reproduction

description: Abstract Antioxidants are natural or synthetic substances that delay oxidation through one or more mechanisms, such as scavenging free radicals, inhibiting lipid peroxidation, and complexing with metals, inhibiting tissue destruction via oxidation. Antioxidants are commonly used in animal feed and the food industry to prevent the oxidation of animal-origin products. Moreover, natural oxidants are used increasingly in animal reproduction, especially for semen preservation. In this context, this study aimed to review the applications of natural antioxidants in animal reproduction. We observed that the bulk of the natural antioxidants, approximately 80.4%, were commercially acquired and used mainly for semen cooling/freezing (72%) with promising results (90%) in Sus scrofa (boar), Capra aegagrus hircus (goat), Gallus gallus domesticus (rooster), and Ovis aries (ram). However, further studies are needed to help determine the appropriate dosage of natural antioxidants for applications.
DOI: 10.6084/m9.figshare.21679068.v1
title: Application of natural antioxidants in animal reproduction

description: Abstract Antioxidants are natural or synthetic substances that delay oxidation through one or more mechanisms, such as scavenging free radicals, inhibiting lipid peroxidation, and complexing with metals, inhibiting tissue destruction via oxidation. Antioxidants are commonly used in animal feed and the food industry to prevent the oxidation of animal-origin products. Moreover, natural oxidants are used increasingly in animal reproduction, especially for semen preservation. In this context, this study aimed to review the applications of natural antioxidants in animal reproduction. We observed that the bulk of the natural antioxidants, approximately 80.4%, were commercially acquired and used mainly for semen cooling/freezing (72%) with promising results (90%) in Sus scrofa (boar), Capra aegagrus hircus (goat), Gallus gallus domesticus (rooster), and Ovis aries (ram). However, further studies are needed to help determine the appropriate dosage of natural antioxidants for applications.
DOI: 10.6084/m9.figshare.21679071
title: Periods of coexistence of weeds with carrot grown with and without black oat straw

description: Abstract The objective of this work was to evaluate the effect of black oat (Avena strigosa) straw on the period prior to the interference (PPI) of weeds in the 'Alvorada' carrot (Daucus carota) crop. The experiment was carried out in a randomized complete block design, in a 2×5 factorial arrangement, with five replicates. The following two factors were evaluated in the 2016/2017 and 2017/2018 crop years: soil cover, with and without 10 Mg ha1 black oat straw; and periods of coexistence with weeds – 0, 15, 30, 60, and 120 days after emergence (DAE). To determine the PPI, a loss of 5% in the commercial yield of carrot roots was considered as acceptable. At the end of the crop cycle, straw reduced the total dry mass of weeds by 43.5 and 67.5%, respectively, in the first and second crop years. The PPI of the crops with and without straw was, respectively, 2.0 and 1.5 DAE in the first crop year and 17 and 6.0 DAE in the second. The use of black oat straw increases the PPI for the 'Alvorada' carrot crop.
DOI: 10.6084/m9.figshare.21679071.v1
title: Periods of coexistence of weeds with carrot grown with and without black oat straw

description: Abstract The objective of this work was to evaluate the effect of black oat (Avena strigosa) straw on the period prior to the interference (PPI) of weeds in the 'Alvorada' carrot (Daucus carota) crop. The experiment was carried out in a randomized complete block design, in a 2×5 factorial arrangement, with five replicates. The following two factors were evaluated in the 2016/2017 and 2017/2018 crop years: soil cover, with and without 10 Mg ha1 black oat straw; and periods of coexistence with weeds – 0, 15, 30, 60, and 120 days after emergence (DAE). To determine the PPI, a loss of 5% in the commercial yield of carrot roots was considered as acceptable. At the end of the crop cycle, straw reduced the total dry mass of weeds by 43.5 and 67.5%, respectively, in the first and second crop years. The PPI of the crops with and without straw was, respectively, 2.0 and 1.5 DAE in the first crop year and 17 and 6.0 DAE in the second. The use of black oat straw increases the PPI for the 'Alvorada' carrot crop.
DOI: 10.6084/m9.figshare.21679074
title: Impact of COVID-19 on maxillofacial surgery practice: a systematic review

description: Abstract Objectives: The aim of this systematic review was to evaluate the possible impacts of COVID-19 on oral and maxillofacial surgery practice, as well as the protocols employed by oral and maxillofacial surgeons to minimize the risks of contamination. Methods: Searches were performed in different databases up to 15 March 2021. Articles related to oral surgery dynamics within the context of the COVID-19 pandemic were included. The articles were considered eligible if they met the following inclusion criteria: observational studies that provided recommendations regarding maxillofacial surgery measures during the COVID-19 pandemic and that analyzed the risk of contamination of patients/professionals with SARS-CoV-2. Results: Seven studies met the inclusion criteria and were selected for the present systematic review. Demands for personal protective equipment increased significantly, with studies reporting the scarcity of fundamental equipment such as FFP2/N95 masks. Significant changes in the infrastructure of outpatient, surgical and inpatient units and in the care protocols themselves were observed. Conclusion: The COVID-19 pandemic particularly affected the routine of oral and maxillofacial surgery residents. Several additional recommendations for surgical practice or for the surgical team were evaluated and discussed, such as performing primarily urgent and emergency surgeries, remote patient care and screening, postponing elective surgeries, and new teaching methods in oral and maxillofacial surgery residency programs. In addition, screening of patients and of the surgery team for COVID-19 is strongly recommended.
DOI: 10.6084/m9.figshare.21679074.v1
title: Impact of COVID-19 on maxillofacial surgery practice: a systematic review

description: Abstract Objectives: The aim of this systematic review was to evaluate the possible impacts of COVID-19 on oral and maxillofacial surgery practice, as well as the protocols employed by oral and maxillofacial surgeons to minimize the risks of contamination. Methods: Searches were performed in different databases up to 15 March 2021. Articles related to oral surgery dynamics within the context of the COVID-19 pandemic were included. The articles were considered eligible if they met the following inclusion criteria: observational studies that provided recommendations regarding maxillofacial surgery measures during the COVID-19 pandemic and that analyzed the risk of contamination of patients/professionals with SARS-CoV-2. Results: Seven studies met the inclusion criteria and were selected for the present systematic review. Demands for personal protective equipment increased significantly, with studies reporting the scarcity of fundamental equipment such as FFP2/N95 masks. Significant changes in the infrastructure of outpatient, surgical and inpatient units and in the care protocols themselves were observed. Conclusion: The COVID-19 pandemic particularly affected the routine of oral and maxillofacial surgery residents. Several additional recommendations for surgical practice or for the surgical team were evaluated and discussed, such as performing primarily urgent and emergency surgeries, remote patient care and screening, postponing elective surgeries, and new teaching methods in oral and maxillofacial surgery residency programs. In addition, screening of patients and of the surgery team for COVID-19 is strongly recommended.
DOI: 10.6084/m9.figshare.21679077
title: Evidence from Cochrane systematic reviews on pharmacological treatment compared to placebo for panic disorder

description: ABSTRACT. Panic disorder is an anxiety condition characterized by recurrent and unexpected panic attacks. The comparison between active treatment and placebo is essential to analyze an intervention’s efficacy and safety. It is important to identify and summarize the studies with higher evidence to assist health professionals and public policy managers in clinical decision-making. Objective: The aim of this study was to identify and summarize all Cochrane systematic reviews (SRs) that compared the efficacy and safety of any drug treatment compared to placebo for panic disorder patients. Methods: SRs published in the Cochrane Library were included without date restriction. All outcomes presented were analyzed. The methodological quality of the SRs was evaluated using the AMSTAR-2 tool. Results: We included three Cochrane SRs of high methodological quality on the effects of antidepressants, benzodiazepines, and azapirones for panic disorder. All medications showed benefits in response to treatment, symptom improvement, and reduced panic attacks. Dropouts were lower with tricyclic antidepressants and benzodiazepines and higher with azapirones. The occurrence of adverse events was higher for drug groups. Conclusions: Very low to moderate certainty evidence (GRADE) showed that antidepressants and benzodiazepines seem to improve clinical symptoms in individuals with short-term panic disorder compared to placebo. In addition, the use of azapirones seems to have greater adherence by patients than placebo. However, there is insufficient evidence to support its clinical efficacy.
DOI: 10.6084/m9.figshare.21679077.v1
title: Evidence from Cochrane systematic reviews on pharmacological treatment compared to placebo for panic disorder

description: ABSTRACT. Panic disorder is an anxiety condition characterized by recurrent and unexpected panic attacks. The comparison between active treatment and placebo is essential to analyze an intervention’s efficacy and safety. It is important to identify and summarize the studies with higher evidence to assist health professionals and public policy managers in clinical decision-making. Objective: The aim of this study was to identify and summarize all Cochrane systematic reviews (SRs) that compared the efficacy and safety of any drug treatment compared to placebo for panic disorder patients. Methods: SRs published in the Cochrane Library were included without date restriction. All outcomes presented were analyzed. The methodological quality of the SRs was evaluated using the AMSTAR-2 tool. Results: We included three Cochrane SRs of high methodological quality on the effects of antidepressants, benzodiazepines, and azapirones for panic disorder. All medications showed benefits in response to treatment, symptom improvement, and reduced panic attacks. Dropouts were lower with tricyclic antidepressants and benzodiazepines and higher with azapirones. The occurrence of adverse events was higher for drug groups. Conclusions: Very low to moderate certainty evidence (GRADE) showed that antidepressants and benzodiazepines seem to improve clinical symptoms in individuals with short-term panic disorder compared to placebo. In addition, the use of azapirones seems to have greater adherence by patients than placebo. However, there is insufficient evidence to support its clinical efficacy.
DOI: 10.6084/m9.figshare.21679076
title: Technological innovation territories and networks: experiences in Buenos Aires and Seville

description: Abstract The article analyzes policies for the formation of technology districts and parks, understood as innovation means or networks. It compares two cases located in disparate geographical contexts and temporalities, but with complementary purposes: on the one hand, the Cartuja Science and Technology Park in Seville, Spain; on the other, the Technology District in Buenos Aires, Argentina. The methodological proposal includes a systematization of regulations and official documents, along with visits to the territory, photographic records, and interviews with key informants from each district. The results subsidize an analysis of territorial registration and effective conformation of these means of innovation, their articulation with the social and economic needs of the immediate environment, and the purposes of internationalization.
DOI: 10.6084/m9.figshare.21679076.v1
title: Technological innovation territories and networks: experiences in Buenos Aires and Seville

description: Abstract The article analyzes policies for the formation of technology districts and parks, understood as innovation means or networks. It compares two cases located in disparate geographical contexts and temporalities, but with complementary purposes: on the one hand, the Cartuja Science and Technology Park in Seville, Spain; on the other, the Technology District in Buenos Aires, Argentina. The methodological proposal includes a systematization of regulations and official documents, along with visits to the territory, photographic records, and interviews with key informants from each district. The results subsidize an analysis of territorial registration and effective conformation of these means of innovation, their articulation with the social and economic needs of the immediate environment, and the purposes of internationalization.
DOI: 10.6084/m9.figshare.21679080
title: Solid waste generation indexes in a port site

description: Abstract The construction industry is one of the largest consumers of natural resources. As it is in a marine environment and due to its magnitude and the high production of Construction and Demolition Waste (CDW), port works have a polluting potential and the technical-scientific literature on the subject is incipient. The objective of this research was to critically evaluate waste generation indexes of CDW during the construction of a port. The monitoring of the solid waste management from the Dom Pedro II port site in Paranaguá, Paraná, was carried out for 20 months. Through the assessment applicable to waste management and field measurements, the solid waste management process was characterized in terms of the agents involved, origin, type, quantity, generation rate, reuse, and destination. The efficiency of the management process was evaluated, and waste generation and utilization of Key Performance Indicators, per capita and per area, were obtained. The composition, in mass, of the solid waste generated in the work was: metallic scrap (48%), wood scrap (29%), organic waste (10%), recyclable waste (8%), and hazardous waste (4%). Per capita monthly waste generation indicators were calculated as: organic waste (12.75 kg), outpatient waste (8.14g), hazardous waste (5.33kg), plastic cups (70.5 units), and PPE (3 .89 units). As for the built-up area, the average monthly generation of waste was calculated at 0.35 kg/m² (hazardous waste), 3.51 kg/m² (wood), 7.5 kg/m² (metallic scrap), and 0.97 kg /m² (organic). In addition to mapping opportunities for improvement, the calculated generation and utilization indexes can be used in the CDW management of similar works.
DOI: 10.6084/m9.figshare.21679080.v1
title: Solid waste generation indexes in a port site

description: Abstract The construction industry is one of the largest consumers of natural resources. As it is in a marine environment and due to its magnitude and the high production of Construction and Demolition Waste (CDW), port works have a polluting potential and the technical-scientific literature on the subject is incipient. The objective of this research was to critically evaluate waste generation indexes of CDW during the construction of a port. The monitoring of the solid waste management from the Dom Pedro II port site in Paranaguá, Paraná, was carried out for 20 months. Through the assessment applicable to waste management and field measurements, the solid waste management process was characterized in terms of the agents involved, origin, type, quantity, generation rate, reuse, and destination. The efficiency of the management process was evaluated, and waste generation and utilization of Key Performance Indicators, per capita and per area, were obtained. The composition, in mass, of the solid waste generated in the work was: metallic scrap (48%), wood scrap (29%), organic waste (10%), recyclable waste (8%), and hazardous waste (4%). Per capita monthly waste generation indicators were calculated as: organic waste (12.75 kg), outpatient waste (8.14g), hazardous waste (5.33kg), plastic cups (70.5 units), and PPE (3 .89 units). As for the built-up area, the average monthly generation of waste was calculated at 0.35 kg/m² (hazardous waste), 3.51 kg/m² (wood), 7.5 kg/m² (metallic scrap), and 0.97 kg /m² (organic). In addition to mapping opportunities for improvement, the calculated generation and utilization indexes can be used in the CDW management of similar works.