DOI: 10.5061/dryad.zcrjdfnph
title: Confocal microscopy images for: Surface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms

description: Biofilms are ubiquitous surface-associated bacterial communities embedded
 in an extracellular matrix. It is commonly assumed that biofilm cells are
 glued together by the matrix; however, how the specific biochemistry of
 matrix components affects the cell-matrix interactions and how these
 interactions vary during biofilm growth remain unclear. Here, we
 investigate cell-matrix interactions in Vibrio cholerae, the causative
 agent of cholera. We combine genetics, microscopy, simulations, and
 biochemical analyses to show that V. cholerae cells are not attracted to
 the main matrix component (Vibrio polysaccharide, VPS), but can be
 attached to each other and to the VPS network through crosslinks formed by
 protein Bap1. Downregulation of VPS production and surface trimming by
 polysaccharide lyase RbmB cause surface remodeling as biofilms age,
 shifting the nature of cell-matrix interactions from attractive to
 repulsive and facilitating cell dispersal as aggregated groups. Our
 results shed light into the dynamics of diverse cell-matrix interactions
 as drivers of biofilm development.
description: All datasets were acquired using a Yokogawa CSU-W1 spinning-disk
 confocal scanning unit mounted on a Nikon Ti2-E microscope body, using the
 Nikon perfect focus system and images were acquired using Nikon Elements
 5.20. For time course imaging, cells were incubated in a Tokai-Hit
 stage-top incubator at a temperature of 25°C. Provided here are raw data
 in nd2 format, which can be opened with FIJI.  Dataset
 1: For this data, a 60× water immersion objective (numerical aperture =
 1.20) was used. FM 4-64 at 2 µg/mL was used to stain cell membranes and
 imaged at 561 nm. Time difference between images is 30 minutes. 
    Dataset 2: For this data, a 100× silicon oil
 immersion objective (Lambda S 100XC Sil, numerical aperture = 1.35) was
 used.  FM 4-64 at 2 µg/mL was used to stain cell membranes and imaged at
 561 nm. Cells consistutively express SCFP3A and were imaged at 445 nm,
 although the signals were weak. VPS was stained with wheat germ agglutinin
 (WGA) conjugated to Oregon Green (4 µg/mL). Time difference between images
 is 30 minutes.     Dataset 3: For this data, a 100×
 silicon oil immersion objective (Lambda S 100XC Sil, numerical aperture =
 1.35) was used. A z-step size of 0.13 μm was used. FM 4-64 at 2 µg/mL was
 used to stain cell membranes and imaged at 561 nm.  VPS was stained with
 wheat germ agglutinin (WGA) conjugated to AlexaFluor647 (4 µg/mL).
 Bap1-GFP was imaged at 488 nm. 
description: # Confocal microscopy images for: Surface remodeling and inversion of
 cell-matrix interactions underlie community recognition and dispersal in
 Vibrio cholerae biofilms
 [https://doi.org/10.5061/dryad.zcrjdfnph](https://doi.org/10.5061/dryad.zcrjdfnph) ## Description of the data and file structure Confocal microscopy images for Vibrio cholerae biofilm for: Surface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms ### Files and variables #### File: Dataset\_1\_Fig1b\_Timecourse.nd2 **Description:** Raw data for Figure 1b.  #### File: Dataset\_2\_Fig4a\_Timecourse.nd2 **Description:** Raw data for Figure 4a. #### File: Dataset\_3\_Fig3c\_VPS-Bap1.nd2 **Description:** Raw data for Figure 3c.  ## Code/software FIJI 2.16.0 can be used to open the nd2 files.