cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.v1s3s
title: Data from: Rapid increase in dispersal during range expansion in the invasive ladybird Harmonia axyridis

description: The evolutionary trajectories associated with demographic, genetic and
 spatial disequilibrium have become an issue of growing interest in
 population biology. Invasive species provide unique opportunities to
 explore the impact of recent range expansion on life-history traits,
 making it possible to test for a spatial arrangement of dispersal
 abilities along the expanding range, in particular. We carried out
 controlled experiments in laboratory conditions to test the hypothesis of
 an increase in dispersal capacity with range expansion in Harmonia
 axyridis, a ladybird that has been invading Europe since 2001. We found a
 marked increase in the flight speed of the insects from the core to the
 front of the invasion range in two independent sampling transects. By
 contrast, we found that two other traits associated with dispersal
 (endurance and motivation to fly off) did not follow the same spatial
 gradient. Our results provide a striking illustration of the way in which
 predictable directional genetic changes may occur rapidly for some traits
 associated with dispersal during biological invasions. We discuss the
 consequences of our results for invasion dynamics and the evolutionary
 outcomes of spatially expanding populations.
description: Phenotypic and genotypic
 data - Asian ladybird Harmonia axyridisLadybirds (Harmonia
 axyridis) were collected in the field in october/november 2010 in France
 and Belgium. The file
 "FLIGHT_SPEED_ENDURANCE_Lombaert_et_al_JEB-2013-00585.txt"
 contains the phenotypic data of the flight speed and endurance experiment.
 They were used and described in the article "Rapid increase in
 dispersal during range expansion in the invasive ladybird Harmonia
 axyridis". Columns titles are as described in the methods section.
 The file "MOTIVATION_TO_FLY_Lombaert_et_al_JEB-2013-00585.txt"
 contains the phenotypic data of the motivation to fly off experiment. They
 were used and described in the article "Rapid increase in dispersal
 during range expansion in the invasive ladybird Harmonia axyridis".
 Columns titles are as described in the methods section. The file
 "GENOTYPES_Lombaert_et_al_JEB-2013-00585.txt" contains the
 genotypes of 247 individuals from 8 population samples of the Asian
 Ladybird Harmonia axyridis (GENEPOP file). They were used and described in
 the article "Rapid increase in dispersal during range expansion in
 the invasive ladybird Harmonia axyridis". Population samples names
 are as in Figure 1 and Table S1.Lombaert_et_al_JEB-2013-00585.zip
DOI: 10.5061/dryad.8mm4h
title: Data from: Restoration of native mangrove wetlands can reverse diet shifts of benthic macrofauna caused by invasive cordgrass

description: 1. Ecological replacement using native mangrove species combined with
 physical treatments has become an effective method in controling the
 spread of invasive Spartina alterniflora. To re-establish ecosystem
 functions, trophic interactions between macrofauna and their potential
 food resources must be considered during the restoration process. 2. Here
 we examined the changes in the diets of macrofauna in three restored
 mangrove ecosystems with different invasion histories following the
 removal of S. alterniflora in southern China. Carbon and nitrogen stable
 isotope ratios for dominant macrofauna, as well as their potential food
 sources, were analyzed. The relative contributions of the different carbon
 sources to the diets of benthic macrofauna and isotopic niche width,
 including convex hull areas and standard ellipse areas of the macrofaunal
 community, were then calculated and compared among sampling sites in each
 region. 3. Our results indicated that Spartina-derived detritus
 contributed to &gt;80% of the food sources of dominant macrofauna in
 the S. alterniflora communities in all three regions. S. alterniflora
 communities had lower convex hull areas and standard ellipse areas than
 natural mangrove forests, indicating significant resource competition
 among different consumer populations. Replacing S. alterniflora with the
 native mangrove species Kandelia obovata could reverse the diets of these
 macrofaunal species, resulting in a shift from homogeneous
 Spartina-dominated diets to more heterogeneous algae-based diets. It could
 take several decades to restore food web interactions to a pre-impacted
 state. Even the diets of macrofauna in mature mangrove (&gt;40 years)
 remained affected by the Spartina-derived organic matter. 4. Synthesis and
 applications: Our study revealed that the ecological replacement of
 invasive Spartina alterniflora using native mangrove species could restore
 food web function gradually. However, this removal and replacement
 approach is a long process and requires significant manpower and
 resources. Furthermore, the native ecosystem will continue to be
 influenced by the cordgrass as long as large areas of wetland nearby are
 occupied by S. alterniflora. Consequently, preventing the colonization of
 S. alterniflora should be a priority for coastal ecosystem management in
 southern China.
description: Feng et al-stablel
 isotope data for Journal of Applied Ecology 2017The datafile include all
 the stable isotoep data of macrofauna and food sources used in the paper.
 Abbreviation:
DOI: 10.6084/m9.figshare.29041502.v1
title: Quaternary ammonium compounds in medicinal herbs and health risk assessment

description: The rising global demand for medicinal herbs has increased human consumption, raising concerns about potential health risks related to quaternary ammonium compounds (QACs), especially among infants due to frequent use in certain cultures. To perform a market survey, an LC-ESI-MS/MS method was developed to detect five QACs, including Benzalkonium Chlorides (BACs) and Dialkyl Dimethyl Ammonium Chloride (DDAC), in dry medicinal herbs. The method employed QuEChERS extraction with acetonitrile and achieved chromatographic separation with a poroshell C18 column. Method validation was conducted according to Eurachem and SANTE/11312/2021 guidelines, showing high sensitivity and selectivity, with recoveries between 70% and 110% and RSDs below 5%. The method was applied to market samples, resulting in 33% exceedances of the maximum residue limits. In this study also dietary exposure risks for adults, toddlers and infants were evaluated. Preliminary risk assessment indicated acceptable dietary exposure risks.
DOI: 10.6084/m9.figshare.28624942
title: Histamine and biogenic amines in Vietnamese traditional fish sauce by hydrophilic interaction liquid chromatography-tandem mass spectrometry

description: A straightforward method for the determination of histamine and other biogenic amines in Vietnamese traditional fish sauce was developed, avoiding a time-consuming derivatisation step. This resulted in the application of hydrophilic interaction in combination with liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The characteristics of the analytical method, such as linearity, limit of detection (LOD), limit of quantification (LOQ), repeatability, stability, and matrix effects were systematically investigated. The validated method was successfully applied to analyse 8 biogenic amines in 15 Vietnamese traditional fish sauce samples, as well as 10 broth samples directly collected from the fermentation tanks. Among the eight investigated biogenic amines, histamine was found in all analysed samples, in some at high levels. However, in traditional fish sauce samples collected from supermarkets histamine levels were lower than the maximum level as set by European Union regulations.
DOI: 10.6084/m9.figshare.28624942.v1
title: Histamine and biogenic amines in Vietnamese traditional fish sauce by hydrophilic interaction liquid chromatography-tandem mass spectrometry

description: A straightforward method for the determination of histamine and other biogenic amines in Vietnamese traditional fish sauce was developed, avoiding a time-consuming derivatisation step. This resulted in the application of hydrophilic interaction in combination with liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The characteristics of the analytical method, such as linearity, limit of detection (LOD), limit of quantification (LOQ), repeatability, stability, and matrix effects were systematically investigated. The validated method was successfully applied to analyse 8 biogenic amines in 15 Vietnamese traditional fish sauce samples, as well as 10 broth samples directly collected from the fermentation tanks. Among the eight investigated biogenic amines, histamine was found in all analysed samples, in some at high levels. However, in traditional fish sauce samples collected from supermarkets histamine levels were lower than the maximum level as set by European Union regulations.
DOI: 10.6084/m9.figshare.29041502
title: Quaternary ammonium compounds in medicinal herbs and health risk assessment

description: The rising global demand for medicinal herbs has increased human consumption, raising concerns about potential health risks related to quaternary ammonium compounds (QACs), especially among infants due to frequent use in certain cultures. To perform a market survey, an LC-ESI-MS/MS method was developed to detect five QACs, including Benzalkonium Chlorides (BACs) and Dialkyl Dimethyl Ammonium Chloride (DDAC), in dry medicinal herbs. The method employed QuEChERS extraction with acetonitrile and achieved chromatographic separation with a poroshell C18 column. Method validation was conducted according to Eurachem and SANTE/11312/2021 guidelines, showing high sensitivity and selectivity, with recoveries between 70% and 110% and RSDs below 5%. The method was applied to market samples, resulting in 33% exceedances of the maximum residue limits. In this study also dietary exposure risks for adults, toddlers and infants were evaluated. Preliminary risk assessment indicated acceptable dietary exposure risks.
DOI: 10.5061/dryad.8ft948b
title: Data from: Avian malaria and bird humoral immune response

description: Background: Plasmodium parasites are known to impose fitness costs on
 their vertebrate hosts. Some of these costs are due to the activation of
 the immune response, which may divert resources away from
 self-maintenance. Plasmodium parasites may also immuno-deplete their
 hosts. Thus, infected individuals may be less able to mount an immune
 response to a new pathogen than uninfected ones. However, this has been
 poorly investigated. Methods: The effect of Plasmodium infection on bird
 humoral immune response when encountering a novel antigen was tested. A
 laboratory experiment was conducted on canaries (Serinus canaria)
 experimentally infected with Plasmodium relictum (lineage SGS1) under
 controlled conditions. Birds were immune challenged with an intra-pectoral
 injection of a novel non-pathogenic antigen (keyhole limpet haemocyanin,
 KLH). One week later they were challenged again. The immune responses to
 the primary and to the secondary contacts were quantified as anti-KLH
 antibody production via enzyme-linked immunosorbent assay (ELISA).
 Results: There was no significant difference in antibody production
 between uninfected and Plasmodium infected birds at both primary and
 secondary contact. However, Plasmodium parasite intensity in the blood
 increased after the primary contact with the antigen. Conclusions: There
 was no effect of Plasmodium infection on the magnitude of the humoral
 immune response. However, there was a cost of mounting an immune response
 in infected individuals as parasitaemia increased after the immune
 challenge, suggesting a trade-off between current control of chronic
 Plasmodium infection and investment against a new immune challenge.
description: complete data
 setcomplete data set from
 the laboratory experiment. the sheet entitled "measurements_bird
 level" contains all bird individual measurements. the sheet entitled
 "food consumption_cage level" contains all food consumption
 measurements at the cage level.Delhaye et al_Malaria J_avian
 malaria immune response_data.xlsx
DOI: 10.5061/dryad.3pc7c
title: Data from: Local human pressures influence gene flow in a hybridizing Daphnia species complex

description: Anthropogenic environmental changes are considered critical drivers of the
 genetic structure of populations and communities through, for example, the
 facilitation of introgressive hybridization between syntopic species.
 However, the mechanisms by which environmental perturbations trigger
 changes in the genetic structure of populations and communities, such as
 the processes that determine the directionality of hybridization and
 patterns of mitochondrial introgression over many generations, remain
 largely unexplored. In this study, the changes in genetic structure of
 hybridizing members of the Daphnia longispina species complex were
 reconstructed over the last 100 years for three large temperate lakes
 under strong anthropogenic pressures via paleogenetic analyses of resting
 egg banks. Drastic changes in the genetic structure of the Daphnia
 community, associated with hybridization events between D. longispina and
 D. galeata and subsequent introgression, were detected in Lakes Geneva and
 Bourget. In Lake Bourget, these changes were induced by the successful
 establishment of D. galeata with rising phosphorus levels and reinforced
 by the sensitivity of D. longispina to fish predation pressure. In Lake
 Geneva, the pattern of hybridization during eutrophication is more likely
 a function of the original taxonomic composition of the species complex in
 this lake. Lakes seem to require at least a meso-oligotrophic status to
 allow D. galeata populations to establish and accordingly no D. galeata
 genotypes were found in the egg bank of oligotrophic Lake Annecy. In
 contrast to the generally assumed pattern of unidirectional hybridization
 in this species complex, bidirectional hybridization was recorded in Lakes
 Geneva and Bourget. Our results also demonstrate complex genetic
 trajectories within this species complex and highlight the irreversibility
 of changes in the genotypic architecture of populations driven by local
 human pressures. Finally, we show that extensive hybridization and
 introgression do not necessarily result in a large and homogenous hybrid
 swarm.
description: Alricetal_micros_genotypingMicrosatellite genotyping for populations of Daphnia longispina species complex in Lakes Geneva, Bourget and AnnecyAlricetal_micros_genotying.txtAlricetal_mtDNA_haplotypesMitochondrial haplotypes for populations of Daphnia longispina species complex in Lakes Genena, Bourget and Annecy.Alricetal_mtDNA_genotyping.txt
DOI: 10.5061/dryad.q1sq0
title: Data from: Identifying management actions to increase foraging opportunities for shorebirds at semi-intensive shrimp farms

description: The expansion of aquaculture has resulted in widespread habitat conversion
 throughout the world. Identifying beneficial management measures may
 dramatically reduce negative impacts of aquaculture for migratory birds.
 We studied how densities of foraging shorebirds varied at ponds within a
 semi-intensive shrimp aquaculture farm on the north-western coast of
 Mexico, as related to timing of harvest and tidal cycles. Further, we
 estimated the total daily available area for each shorebird species
 throughout two entire harvesting seasons at the shrimp farm. High
 densities (average ca. 50 individuals per ha) of foraging shorebirds were
 found during the first days following pond harvest. The most abundant
 species were Willet Tringa semipalmata and Black-necked Stilt Himantopus
 mexicanus, followed by Marbled Godwit Limosa fedoa and American Avocet
 Recurvirostra americana. Other regular, but less abundant, species were
 whimbrel Numenius phaeopus and dowitchers Limnodromus spp. Densities of
 shorebirds sharply declined daily following harvest. In addition, the
 time-window availability of harvested ponds was related to each
 species' foraging behaviour: &lt;2 days for godwits and
 dowitchers, 4 days for stilts, 5 days for willets and more than a week for
 avocets and whimbrels. However, birds continued to use harvested ponds
 that received a low, but continuous water influx. Our results demonstrate
 that a tropical shrimp farm represents a patchy environment that provides
 4·3–12·7% (depending on species) of its total area as foraging
 opportunities for shorebirds. Synthesis and applications. Sequential
 harvesting of shrimp aquaculture farm ponds and increasing moisture of the
 substrate by providing some water supply to harvested ponds, at least
 throughout the harvesting period, could integrate shorebird conservation
 into shrimp-farm production. These low-cost and easy management procedures
 would not increase costs or affect shrimp production – thus representing a
 win-win opportunity – and would have potential applications for shorebird
 conservation throughout the world.
description: data_bird_density_2script_density
DOI: 10.5061/dryad.pn1j6
title: Data from: The effect of egg size on hatch time and metabolic rate: theoretical and empirical insights on developing insect embryos

description: Body size scaling relationships allow biologists to study ecological
 phenomena in terms of individual level metabolic processes. Recently,
 dynamic energy budget (DEB) theory has been shown to offer novel insights
 on the effect of body size on biological rates. We test whether DEB theory
 and its unique partitioning of biomass into reserve and structural
 components can explain the effect of egg size on hatch time and the time
 course of respiration in insect embryos. We find that without any
 parameterization (calibration), DEB theory is able to predict hatch time
 for eggs sizes spanning four orders of magnitude from fundamental
 biological processes. We find, however, that the standard DEB model poorly
 predicts the time course of respiration, particularly in early embryonic
 development where a strong effect of egg size is observed. Further, we
 show that other theoretical models also poorly predict early embryonic
 respiration. By modifying the assumption that a fresh egg is entirely
 reserve, we show that embryonic respiration and hatch time can be better
 predicted by the DEB model. Useful theories in metabolic ecology, such as
 DEB theory, can help explain universal scaling patterns in development
 times. However, simple theoretical models must be expanded if they are to
 capture the scaling of metabolic rate in insect eggs.
description: Insect egg size and
 hatch timeThis data set consists
 of compiled data from 68 sources on insect egg size and hatch time for 98
 species from 12 insect orders (Coleoptera, Hemiptera, Lepidoptera,
 Orthoptera, Megaloptera, Hymenoptera, Diptera, Ephemeroptera, Heteroptera,
 Odonata, Plecoptera, and Trichoptera). The range of egg masses in the data
 set spans five orders of magnitude.EggHatchTimeData.xlsx
DOI: 10.5061/dryad.15r7f
title: Data from: MHC variability in an isolated wolf population in Italy

description: Small, isolated populations may experience increased extinction risk due
 to reduced genetic variability at important functional genes, thus
 decreasing the population’s adaptive potential. The major
 histocompatibility complex (MHC), a key immunological gene cluster,
 usually shows high variability maintained by positive or balancing
 selection in response to challenges by pathogens. Here we investigated for
 the first time, the variability of 3 MHC class II genes (DRB1, DQA1, and
 DQB1) in 94 samples collected from Italian wolves. The Italian wolf
 population has been long isolated south of the Alps and is presently
 recovering from a recent bottleneck that decreased the population to less
 than 100 individuals. Despite the bottleneck, Italian wolves show
 remarkable MHC variability with 6–9 alleles per locus, including 2
 recently described alleles at DRB1. MHC sequences show signatures of
 historical selective pressures (high d N/d S ratio, ω &gt; 1.74) but
 no evidence of ongoing selection. Variation at the MHC genes and 12
 background microsatellite loci were not apparently affected by the recent
 bottleneck. Although MHC alleles of domestic dog origin were detected in 8
 genetically admixed individuals, these alleles were rare or absent in
 nonadmixed wolves. Thus, despite known hybridization events between
 domestic dogs and Italian wolves, the Italian wolf population does not
 appear affected by deep introgression of domestic dog MHC alleles.
description: MHC alleles and STR
 genotypes of wolves in ItalyGalaverni_et_al_2013_STR_MHC.xlsx
DOI: 10.5061/dryad.39q83
title: Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus

description: The smooth-hounds represent a significant proportion of the elasmobranch
 catch in the Adriatic basin of the Mediterranean Sea, where the common
 (Mustelus mustelus) and blackspotted (Mustelus punctulatus) smooth-hounds
 co-occur. The 2 species share several morphological and morphometric
 characters that lead to frequent misidentification. In order to provide
 information useful for their species identification, we performed a
 morphological identification of several Mustelus specimens to select
 individuals unambiguously attributed to 1 of the 2 species, and assayed
 these with 3 new molecular tests. First, we developed and validated a
 mitochondrial DNA assay based on species-specific amplification of the
 cytochrome c oxidase subunit 1 (COI). Second, a fragment analysis of 15
 microsatellites cross-amplified from several triakid species was performed
 to identify diagnostic loci. Finally, a length difference was identified
 in the internal transcribed spacer 2 (ITS2) region and a diagnostic test
 based on its amplification was established. All the samples classified
 morphologically as M. mustelus and M. punctulatus showed a
 species-specific profile using all the 3 molecular tests. In addition,
 cross-amplification of microsatellites allowed identification of 9 highly
 polymorphic loci that will be useful for the study of the mating system
 and population differentiation of the 2 species.
description: Mustelus_spp_genotypes.txtMustelus_spp_rawdata