DOI: 10.6084/m9.figshare.30881240
title: Data set of Tanaka et al. "Hidden balance in sexual conflict over inbreeding: antagonistic coevolution between male control and female resistance"

description: 1) Data description<br>Data description<br>This dataset contains experimental data collected to investigate sexual conflict over inbreeding in the West Indian sweet potato weevil, Euscepes postfasciatus. The study combined physiological and behavioral experiments to test how inbreeding depression, male accessory gland substances, and kinship influence female mating receptivity and reproductive output.<br>Specifically, the data include:(1) measurements of female reproductive output from inbred (full-sib) and outbred (non-sib) matings to quantify inbreeding depression;(2) results of microinjection experiments testing the effects of concentration and kinship origin of male accessory gland substances on female mating receptivity; and(3) behavioral observations of female remating under natural copulation conditions following mating with kin or non-kin males.<br>All experiments were conducted under controlled laboratory conditions, and the data were used to test predictions derived from theoretical models of sexual conflict over inbreeding.<br>⸻<br>2) Files and variables<br>Files and variables<br>The Dryad repository includes the following data files and analysis scripts:<br>Data files • TanakaData_Exp1.csvData for Experiment 1 (inbreeding depression). Each row represents a female–male pair. • TanakaData_Exp2.csvData for Experiment 2 (microinjection assay of male accessory gland substances). Each row represents an individual female. • TanakaData_Exp3.csvData for Experiment 3 (natural mating assay). Each row represents an individual female.<br>Common variables (may vary slightly among files) • FemaleID: Unique identifier for each female • Kin: Kinship between mating partners (Kin = full-sib, NonKin = non-sib) • Fsize: Female body size (left elytron length, mm) • Fno / OffspringNumber: Number of adult offspring produced • Inj: Injection treatment (K = kin-derived suspension, cNK = non-kin suspension, NK2 = 2× concentration non-kin suspension) • Period / Period2: Time (days) until mating or remating • Fin / Fin3: Event indicator (1 = mating occurred, 0 = censored)<br>Missing values<br>Missing data are indicated as NA. Blank cells are not used.<br>All variable names are consistent with those used in the accompanying R scripts.<br>⸻<br>3) Code / software<br>Code and software<br>All analyses were conducted using R version 4.5.1 (R Core Team, 2025).The following R packages were used: • MASS (for negative binomial and Poisson GLMs) • survival (for survival analysis and Cox proportional hazards models) • survminer (for visualization and pairwise survival comparisons) • ggplot2 (for plotting)<br>The file TanakaCode.R contains all scripts required to reproduce the statistical analyses and figures reported in the manuscript. The workflow is as follows: 1. Load the CSV data files for each experiment. 2. Perform GLM analyses to estimate inbreeding depression (Experiment 1). 3. Conduct survival analyses and pairwise Peto & Peto tests for mating receptivity (Experiments 2 and 3). 4. Generate figures using ggsurvplot() and related plotting functions.<br>All software used is free and open-source.<br>