cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0sj1jzn
title: CIL:39324, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The pellicular pore consists of the invaginated plasma membrane that penetrates the alveolus as well as the epiplasm and ends as a clathrin-coated pit. TEM taken on 3/14/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 23,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j080520n
title: CIL:39344, Passiflora edulis, pollen. In Cell Image Library

description: Preparation: acetolysis
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions; shadowing and plating
Processing history: unprocessed raw data
description: Scanning electron micrograph of passion flower pollen. A related image is CIL:39346.
description: Instrument: FEI XL-30 FEG ESEM. Complete protocol can be found at http://www.dartmouth.edu/~emlab/manuals/sempreps/pollen.html
DOI: 10.6075/j0930shh
title: CIL:39340, Nicotiana alata. In Cell Image Library

description: See http://remf.dartmouth.edu:8080/EM-Wiki/36 for technical protocol
description: Scanning electron microscope image of Nicotiana alata stem cross section. Image shows outer epidermal layer, followed by the cortex and then large vascular bundles. The vascular bundles contain the phloem (nearest the cortex) and xylem.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating
Processing history: unprocessed raw data
DOI: 10.6075/j0s75fns
title: CIL:39338, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: A view of the AZM with basal bodies in longitudinal section and cilia sectioned lengthwise. Bunches of microtubules reside below the basal bodies and lie close to the cytopharyngeal vesicles. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0xs5tpj
title: CIL:39311, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: This view shows a transverse ridge cut with its sloping side lying tangentially in the section plane. The granulo-fibrillar filled peaks are prominent. Striated bands appear sectioned closer to their mid-sections, probably in areas of filament overlap. The outer alveolar membrane is not usually continuous with the inner alveolar membrane at the tips of the ridges. TEM taken on 11/11/70 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 37,700X.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j09w0dvc
title: CIL:39378, Amorphophallus titanum. In Cell Image Library

description: Images collected on a FEI XL-30 FEG ESEM. Sample preparation protocol is available on the Dartmouth EM Facility web site at http://remf.dartmouth.edu:8080/EM-Wiki/36.
description: Transmission electron micrograph of Amorphophallus titanum anther. This cross section shows the locule (cavity where the pollen is located).This image is part of a group that includes CIL: 39377- 39384.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions; uranyl salt; gold
Processing history: sectioned tissue
DOI: 10.6075/j089157m
title: CIL:39310, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: A view of cortical fibers except the observer is looking at a cross-sectioned cell as though from anterior toward the posterior end of the cell; in this view the kd sections are seen on the left side of the ridge. Striated bands always lie closer to the epiplasm than the infraciliary lattice. TEM taken on 12/30/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 33,750X.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j06q1wk9
title: CIL:39335, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.
description: A contractile vacuole in systole. The CV has collapsed into vesicles and tubules. Three CV pores can be seen where the CV meets the pellicle. Smooth membrane cisterna surrounds some cytoplasm, possibly a developing stage of an autophagic vacuole. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 9,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0zw1jm5
title: CIL:39333, Homo sapiens, epithelial cell. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy; constrained iterative deconvolution
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; distribution of a specific nucleic acid sequence
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI)
Processing history: montage
Data qualification: Processed
description: HeLa cells expressing a kinetochore-specific protein were synchronized and released into S phase. At time intervals of 1,3,4,6,and 8 hr, cells were pulsed with BrdU, fixed and processed to label contromeres (red) and replicating DNA (green). Nuclear DNA was stained with DAPI (blue). Z-series image stacks were obtained using widefield microscopy, and the out-of-focus signal removed by constrained iterative deconvolution.
 Each column shows a single nucleus representative of the S-phase stages I - V. The top row (a-e) shows a merge of the red, green and blue signals, and the center row (f-j), the red and green signals only. The red and green signals were processed to reveal centromere-associated DNA replication which peaks in the latter half of S.
description: See Fig 2 in: RD Shelby et al. 2000 Chromatin assembly at kinetochores is uncoupled from DNA replication. J Cell Biol 151:1113-1118.
DOI: 10.6075/j00v8c30
title: CIL:39336, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.
description: This figure shows the connection between the peristome and the food vacuole forming zone where the alveoli end. The cytostome is the opening that connects these two zones. 
 TEM taken on 6/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j09w0dtx
title: CIL:39315, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: In this image a portion of the true surface of the plasma membrane is exposed where the culture medium has been etched away. The IMPs of the plates can also be viewed less distinctly on the true surface of the cell. The edge of the fractured and removed E leaflet is visible. Fractured doublets of the axoneme of a cilium lie nearby. TEM taken on 6/4/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0rr1xmg
title: CIL:39313, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Freeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous plates of IMPs in the P-face of the plasma membrane. Rings and rosette IMPs at the trichocyst exocytic sites are also seen. TEM taken on 10/2/80 by R. Allen with HU 11A operating at 75kV. Neg. 10,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j04q7tb0
title: CIL:39312, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Longitudinal section of a striated band lying superficially to an arm of the infraciliary lattice but not contacting it. A post is seen at the junction of a branch in the infraciliary lattice. TEM taken on 5/21/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 27,750X.
DOI: 10.6075/j0cc0zjd
title: CIL:39334, Physarum polycephalum, plasmodium. In Cell Image Library

description: See Fig 3A in: 
F Haugli et al. 1982 DNA replication in Physarum polycephalum: electron microscopic analysis patterns of DNA replication in the presence of cycloheximide. J Cell Biol 95:323-331.
description: Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; tungsten compound
Processing history: montage
Data qualification: Processed
description: Nuclear DNA was prepared from synchronously replicating nuclei of Physarum polycephalum, mixed with cytochrome c and formamide, and spread on the surface of a hypophase consisting of 10 mM Tris, 1 mM EDTA and 10% formamide. The surface containing the DNA-cytochrome c complexes was picked up on carbon-formvar grids, stained with ethanolic phosphotungstic acid, and rotary shadowed with Pt/Pd prior to TEM examination. Arrows point to replication forks.
DOI: 10.6075/j0wd3zxg
title: CIL:39328, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: 11 micrographs of views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 4 food vacuoles. The helically contracted stalk is seen in cross section through the first turn of the helix. Lateral margins of the trochal band of basal bodies. Body myonemes converge aborally where they will enter the stalk. TEM taken on 4/9/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 2,400X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0ff3rqm
title: CIL:39322, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Immunogold labeled thin frozen section of P. multimicronucleatum. Gold lies over the microtubules of the basal bodies and the doublets of the cilia. Also short segments of the transverse and postciliary microtubules are labeled. TEM taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X.
DOI: 10.6075/j0125s19
title: CIL:39318, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: In addition to plates the plasma membrane contains IMPs on its P-fracture face in ring or parenthesis shape at sites of trichocyst secretion. A ring actually consists of two circles of IMPs and signifies that a trichocyst is docked underneath while a parenthesis means the site has no docked trichocyst. The fracture also exposed some of the E-face of the outer alveolar membrane which is relatively free of IMPs. TEM taken on 6/4/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).