DOI: 10.5281/zenodo.5801267
title: Dynamics of changes in the oral mucosa cellular composition during the regeneration of a wound defect using a protective piezoelectric coating

description: Introduction. The healing of a wound defect is a process based on a complex mechanism of intercellular interaction. The aim of this research was to study changes in the oral mucosa cellular composition during the regeneration of a wound defect with a protective piezoelectric coating and without it.<br> Materials and methods. The study was carried out on 50 Wistar rats, divided into four groups: control - intact rats, rats of experimental groups №1, №2, №3 were subjected to excision of a lip mucous membrane flap with the formation of a wound defect. In animals of experimental group №1, the defect was open, in animals of experimental groups №2 and №3, the wound defect was covered with a polymer membrane without copper modification and with it respectivily. Animals were sacrificed on the 3 rd , 7 th and 12 th day of the study. The qualitative and quantitative changes in the composition of cell populations at the site of the defect<br> were studied using light and electron microscopy.<br> Results. On the 3 rd day of the study, neutrophilic infiltration prevailed in all groups. In the coated groups, a large number of macrophages and fibroblasts were observed, indicating the transition to the next stage of wound regeneration. On the 7 th day, in the 1 st group, extensive neutrophilic and macrophage infiltration persisted, in the 2 nd and 3rd groups, the signs of inflammation decreased, and wound regeneration was active. On the 12 th day in the coated groups, all the studied parameters reached the control values, in the 1st group all values were significantly higher than at the control group, there were violations of the ultrastructural structure of cells.<br> Conclusion. In the course of this study, the patterns of changes in the cellular composition of a wound defect during its regeneration were revealed. The use of the coating promoted accelerated wound healing, which was found during the analysis of changes in the composition of cell populations. It was proved that the closure of the oral mucosa wounds with polymer piezoelectric membranes influenced the restoration of tissue integrity favorably, and, consequently, reduced the risk of postoperative complications.
description: {"references": ["Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: a cellular perspective. Physiol Rev. 2019;99(1):665\u2013706. DOI: 10.1152/physrev.00067.2017.", "Badaraev AD, Koniaeva AD, Krikova SA, Shesterikov EV, Bolbasov EN, Nemoykina AL, Tverdokhlebov SI. Piezoelectric polymer membranes with thin antibacterial coating for the regeneration of oral mucosa. Applied Surface Science. 2020;504:1. DOI: 10.1016/j.apsusc.2019.144068", "Da Silveria Teixeira D, De Figueiredo MAZ, Cherubina K, De Oliveira SD, Salum FG. The topical effect of chlorhexidine and povidone-iodine in the repair of oral wounds. A review. Stomatologija. 2019;21(2)35-41. PMID:32108654.", "Konyaeva AD, Varakuta EY, Leiman AE, Badaraev AD, Bol'basov EN. Effectiveness of non-woven piezoelectric polymer membrane application based on a co-polymer of vinylidene fluoride with tetrafluoroethylene for oral wound closure. Journal of Anatomy and Histopathology. 2020,9(2):40-45. DOI: 10.18499/2225-7357-2020-9-2-40-45 (In Russ.).", "Wang PH, Huang B., Horng HC, Yeh CC, Chen YJ. Wound healing. J. Chin. Med. Assoc. 2018;81(2):94-101. DOI: 10.1016/j.jcma.2017.11.002.", "Periera D, Sequera I. A scarless healing tale: comparing homeostasis and wound healing of oral mucosa with skin and oesophagus. Front cell Dev Bio. 2021(9):682143. DOI:10.2289/fcell.2021682143.", "Xiao T, Yan Z, Xiao S\u00a0et al.\u00a0Proinflammatory cytokines regulate epidermal stem cells in wound epithelialization.\u00a0Stem Cell Res Ther.\u00a02020;232(11). DOI:10.1186/s13287-020-01755-y", "Avishai E, Yeghiazaryan K. Golubnitschaja O. Impaired wound healing: facts and hypotheses for multi-professional considerations in predictive, preventive and personalised medicine.\u00a0EPMA Journal. 2017;8:23\u201333. DOI:10.1007/s13167-017-0081-y", "Serra MB, Barroso WA, NND S, Silva SDN, Borges ACR, Abreu IC et al. From inflammation to current and alternative therapies involved in wound healing. Int J Inflammation. 2017;1\u201317. DOI: 10.1155/2017/3406215", "Kotwal GJ, Chien S. Macrophage differentiation in normal and accelerated wound healing. Results Probl Cell Differ. 2017;62(3):53\u201364. DOI: 10.1007/978-3-319-54090-0_14.", "Shapouri-Moghaddam A, Mohammadian S, Vazini H, Taghadosi M, Esmaeili SA, Mardani F, Seifi B, Mohammadi A, Afshari JT, Sahebkar A. Macrophage plasticity, polarization, and function in health and disease. J Cell Physiol. 2018;233(9):6425-6440. DOI: 10.1002/jcp.26429.", "Komi DEA, Rambasek T, W\u00f6hrl S. Mastocytosis: from a Molecular Point of View.\u00a0Clinic Rev Allerg Immunol. 2018;54:397\u2013411. DOI:10.1007/s12016-017-8619-2", "Komi DEA, Khomtchouk K, Santa Maria PL. A Review of the Contribution of Mast Cells in Wound Healing: Involved Molecular and Cellular Mechanisms. Clin Rev Allergy Immunol. 2020;58(3):298-312. DOI: 10.1007/s12016-019-08729-w.", "Coden ME, Berdnikovs S. Eosinophils in wound healing and epithelial remodeling: Is coagulation a missing link? J Leukoc Biol. 2020;108(1):93-103. DOI: 10.1002/JLB.3MR0120- 390R.", "Wang X, Balaji S, Steen EH, Li H, Rae MM, Blum AJ, Miao Q, Butte MJ, Bollyky PL, Keswani SG. T Lymphocytes Attenuate Dermal Scarring by Regulating Inflammation, Neovascularization, and Extracellular Matrix Remodeling. Adv Wound Care (New Rochelle). 2019;8(11):527-537. DOI: 10.1089/wound.2019.0981.", "S\u00eerbulescu RF, Boehm CK, Soon E, et al. Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions.\u00a0Wound Repair Regen. 2017;25(5):774-791. DOI:10.1111/wrr.12584", "Rousselle P, Braye F, Dayan G. Re-epithelialization of adult skin wounds: cellular mechanisms and therapeutic strategies. Adv Drug Deliv Rev. 2019;146:344\u201365. DOI: 10.1016/j.addr.2018.06.019.", "Kloc M, Ghobrial RM, Wosik J, Lewicka A, Lewicki S, Kubiak JZ. Macrophage functions in wound healing. J Tissue Eng Regen Med. 2019;13(1):99-109. DOI: 10.1002/term.2772.", "Smigiel KS, Parks WC. Macrophages, Wound Healing, and Fibrosis: Recent Insights. Curr Rheumatol Rep. 2018;20(4):17. DOI: 10.1007/s11926-018-0725-5.", "Alim MA, Peterson M, Pejler G. Do Mast Cells Have a Role in Tendon Healing and Inflammation? Cells. 2020;9(5):1134. DOI: 10.3390/cells9051134.", "Ud-Din S, Wilgus TA, Bayat A. Mast Cells in Skin Scarring: A Review of Animal and Human Research.\u00a0Front Immunol. 2020;11:552205. DOI:10.3389/fimmu.2020.552205", "Xu X, Gu S, Huang X, et al. The role of macrophages in the formation of hypertrophic scars and keloids.\u00a0Burns Trauma. 2020;8:006. DOI:10.1093/burnst/tkaa006", "Carlavan I, Bertino B, Rivier M, Martel P, Bourdes V, Motte M, D\u00e9ret S, Reiniche P, Menigot C, Khammari A, Dreno B, Fogel P, Voegel JJ. Atrophic scar formation in patients with acne involves long-acting immune responses with plasma cells and alteration of sebaceous glands. Br J Dermatol. 2018;179(4):906-917. DOI: 10.1111/bjd.16680.", "Li P, Ruan L, Wang R.\u00a0et al.\u00a0Electrospun Scaffold of Collagen and Polycaprolactone Containing ZnO Quantum Dots for Skin Wound Regeneration.\u00a0J Bionic Eng. 2021:1-13. DOI10.1007/s42235-021-00115-7", "Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev. 2019;99(1):665-706. DOI: 10.1152/physrev.00067.2017."]}