DOI: 10.5281/zenodo.17651645
title: Data and code for: A general framework for nitrogen deposition effects on soil respiration in global forests

description: Cen, X., Vitousek, P., He, N. et al. A general framework for nitrogen deposition effects on soil respiration in global forests. Nat Commun (2025). https://doi.org/10.1038/s41467-025-67203-8

 

Abstract

Since the Industrial Revolution, human activities have altered atmospheric nitrogen (N) deposition to global forests, affecting carbon dioxide emissions from soils (soil respiration or SR) – one of the largest land-atmosphere carbon fluxes. However, experimental studies have demonstrated both positive and negative effects of N deposition on SR in global forests, leading to debates on how N deposition increases or decreases SR. We developed a framework for generalizing SR responses to N deposition using synthesized data from 168 N addition experiments worldwide and observed SR across the global natural N deposition gradient. The findings indicate that N deposition decreased SR in 2.9% of global forested areas, particularly in eastern China, western Europe, and the eastern USA. However, the net effect of N deposition increased the global forest SR by ~5% (1.7 ± 0.1 PgC yr–1). If N pollution could be effectively controlled, global forest SR would decrease, potentially contributing to a reduction in the terrestrial carbon emissions.

 

Data description

We systematically compiled annual soil respiration fluxes observed in forests worldwide where N addition experiments were conducted, by searching for relevant literature published before January 1, 2022 in the Web of Science Core Collection (www.webofscience.com) and the China National Knowledge Infrastructure Theses and Dissertations Database (https://oversea.cnki.net/kns?dbcode=CDMD). These databases are often used in global meta-analysis. The search terms used were: forest AND (greenhouse gas OR CO2 OR carbon dioxide OR soil respiration) AND (nitrogen OR fertiliz*). Then, we manually refined the retrieved 9417 studies based on the following criteria: (i) N addition experiment was conducted in the forest ecosystem, with records of site location and N addition dose; (ii) soil CO2 flux was observed in the field and measured using gas chromatography, infrared gas analyzer, or alkali absorption techniques; (iii) where available, we also compiled data on root respiration and microbial respiration measured using the root trenching method. The compiled CO2_exp dataset contains 1112 observations from paired control and experimental plots at 168 sites. To estimate the global forest soil respiration budget, we used soil respiration data observed under natural conditions (without manipulative experiments) from the global Soil Respiration DataBase (SRDB)42. In line with the CO2_exp dataset, we only used data that met the following criteria: (i) soil CO2 fluxes were observed in forest ecosystems with records of site location; (ii) soil CO2 fluxes were observed in the field and measured using gas chromatography, infrared gas analyzer, or alkali absorption techniques. The derived CO2_obs dataset contains 3689 observations from 966 forest sites worldwide. In addition, we collected auxiliary information on environmental factors (climate, N deposition, soil properties) from the same literature. For sites where not all the required information was available, we extracted data from spatial datasets based on site coordinates. Specifically, temperature and precipitation data were obtained from the Climatic Research Unit, University of East Anglia (https://crudata.uea.ac.uk/cru/data/hrg/cru_ts_4.03/). Soil texture data were from the Harmonized World Soil Database (https://www.fao.org/soils-portal/data-hub/soil-maps-and-databases/harmonized-world-soil-database-v12/en/). Soil chemistry data were from a published dataset by Shangguan et al. Global N deposition data were from a published dataset by Ackerman et al. Forest cover data were from the GLASS-GLC project. Forest biome information was extracted from a spatial dataset of the Global Forest Monitoring project.