cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.28227720.v1
title: CRISPR-Cas9-mediated editing of <i>ZmPL1</i> gene improves tolerance to drought stress in maize

description: Maize (<i>Zea mays</i> L.) is a widely grown food crop around the world. Drought stress seriously affects the growth and development process of plants and causes serious damage to maize yield. In the early stage, our research group conducted transcriptome sequencing analysis on the drought-resistant maize inbred line H8186 and screened out a gene with significantly down-regulated expression, Phylloplanin-like (<i>ZmPL1</i>). The <i>ZmPL1</i> gee expression pattern was analyzed under various abiotic stresses, and the results showed that this gene was greatly affected by drought stress. Subcellular localization analysis showed that the protein was localized on the cell membrane. In order to verify the role of <i>ZmPL1</i> in drought stress, we overexpressed <i>ZmPL1</i> in yeast and found that the expression of <i>ZmPL1</i> could significantly increase the drought sensitivity of yeast. Next, <i>ZmPL1</i> transgenic plants were obtained by infecting maize callus using Agrobacterium-mediated method. Under drought stress, compared with overexpression lines, gene-edited lines had higher germination rate and seedling survival rate, lower accumulation of MDA, relative conductivity and ROS, higher antioxidant enzyme activity, and the expression levels of stress-related genes and ROS scavenging-related genes were significantly increased. Exogenous application of ABA to each lines under drought stress attenuated the damage caused by drought stress on <i>ZmPL</i> overexpressing plants. In summary, <i>ZmPL1</i> negatively regulates drought tolerance in maize.
DOI: 10.6084/m9.figshare.29412512
title: CRISPR/Cas9-mediated editing of barley lipoxygenase genes promotes grain fatty acid accumulation and storability

description: Plant lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids, which can adversely affect grain storability. Although the genetic engineering of <i>LOXs</i> holds great potential for improving grain storage quality, this approach remains largely unexplored in barley. In this study, we identified five <i>LOX</i> genes in the barley genome: <i>HvLOXA</i>, <i>HvLOXB</i>, and <i>HvLOXC1–3</i>. <i>HvLOXC1</i> exhibited the highest expression in early developing grains, roots, and shoots; <i>HvLOXA</i> was predominantly expressed in embryos, whereas <i>HvLOXB</i> and <i>HvLOXC3</i> were weakly expressed across tissues. Transgene-free homozygous barley mutants of <i>loxB</i>, <i>loxC1</i>, and <i>loxAloxC1</i> were generated using CRISPR/Cas9-mediated genome editing. Compared to the wild-type, all mutants displayed normal plant height, tiller number, and grain size, although the <i>loxC1</i> and <i>loxAloxC1</i> mutants exhibited significantly lower thousand grain weights. Notably, the total LOX activity in mature grains decreased by 36–42% in <i>loxC1</i> mutants and by 94% in <i>loxAloxC1</i> mutants, with no significant change observed in <i>loxB</i> mutants. Additionally, the <i>loxAloxC1</i> double mutants had a significantly lower malondialdehyde content and accumulated 10–21% more fatty acids than the wild-type. Artificial aging treatment experiments revealed that <i>loxAloxC1</i> mutants had enhanced grain storability, demonstrated by significantly higher germination rates, reduced lipid peroxidation, and improved seedling growth. Our findings highlight that the targeted knockout of <i>LOX</i> genes, particularly the double mutation of <i>HvLOXA</i> and <i>HvLOXC1</i>, represents a promising genetic strategy for improving grain storability and nutritional value in barley.
DOI: 10.15468/k3yvut
title: Kurzbeitrag Eudarcia hedemanni (Rebel, 1899) - eine neue Tineidae (Lepidoptera) für die Schweiz

description: This dataset contains the digitized treatments in Plazi based on the original journal article Bolt, Daniel, Wittland, Wolfgang (2018): Kurzbeitrag Eudarcia hedemanni (Rebel, 1899) - eine neue Tineidae (Lepidoptera) für die Schweiz. Entomo Helvetica 11: 165-166, DOI: 10.5169/seals-986035Abstract: Eudarcia hedemanni (Rebel, 1899) is reported as new for the fauna of Switzerland (Lepidoptera).Résumé: Eudarcia hedemanni (Rebel, 1899) est signalé comme nouveau pour la faune de Suisse (Lepidoptera).Keywords: New records, faunistics, Tineidae, SwitzerlandDie Familie der Echten Motten (Tineidae) ist eine schwierig zu bestimmende Familie von Kleinschmetterlingen. Insbesondere bei den kleineren Arten der Gattungen Eudarcia und Infurcitinea ist eine sichere Bestimmung nur mittels Genitaluntersuchung möglich. Mit dem Buchwerk Tineidae I von Gaedike (2015), als Teil der Serie «Microlepidoptera of Europe», existiert seit dem Jahr 2015 für die Unterfamilie Meesiinae in der die beiden Gattungen enthalten sind ein sehr gutes Bestimmungsbuch, in welchem auch die zu dem Zeitpunkt zur Verfügung stehenden Genitalien der Männchen und Weibchen abgebildet sind und somit die Bestimmung erleichtern.Während eines gemeinsamen Lichtfangs mit dem Neuroptera-Spezialisten Peter Duelli erbeutete der Erstautor auch einige Kleinschmetterlinge und präparierte diese. Die Bestimmung einer dieser Kleinschmetterlinge wurde vorerst aufgeschoben. Im Rahmen eines Arbeitstreffens von Kleinschmetterlings-Spezialisten der Schweiz wurde das Belegexemplar vorgelegt. Der Zweitautor beschloss das Tier zur weiteren Bestimmung und Genitaluntersuchung mitzunehmen. Die Bestimmung ergab die bis dahin nicht für die Schweiz gemeldete Eudarcia hedemanni (Rebel, 1899) (SwissLepTeam 2010). Für die Bestätigung des Neufundes für die Schweiz wurde der Beleg auch dem Spezialisten Reinhard Gaedike vorgelegt.Daten
DOI: 10.6084/m9.figshare.29412512.v1
title: CRISPR/Cas9-mediated editing of barley lipoxygenase genes promotes grain fatty acid accumulation and storability

description: Plant lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids, which can adversely affect grain storability. Although the genetic engineering of <i>LOXs</i> holds great potential for improving grain storage quality, this approach remains largely unexplored in barley. In this study, we identified five <i>LOX</i> genes in the barley genome: <i>HvLOXA</i>, <i>HvLOXB</i>, and <i>HvLOXC1–3</i>. <i>HvLOXC1</i> exhibited the highest expression in early developing grains, roots, and shoots; <i>HvLOXA</i> was predominantly expressed in embryos, whereas <i>HvLOXB</i> and <i>HvLOXC3</i> were weakly expressed across tissues. Transgene-free homozygous barley mutants of <i>loxB</i>, <i>loxC1</i>, and <i>loxAloxC1</i> were generated using CRISPR/Cas9-mediated genome editing. Compared to the wild-type, all mutants displayed normal plant height, tiller number, and grain size, although the <i>loxC1</i> and <i>loxAloxC1</i> mutants exhibited significantly lower thousand grain weights. Notably, the total LOX activity in mature grains decreased by 36–42% in <i>loxC1</i> mutants and by 94% in <i>loxAloxC1</i> mutants, with no significant change observed in <i>loxB</i> mutants. Additionally, the <i>loxAloxC1</i> double mutants had a significantly lower malondialdehyde content and accumulated 10–21% more fatty acids than the wild-type. Artificial aging treatment experiments revealed that <i>loxAloxC1</i> mutants had enhanced grain storability, demonstrated by significantly higher germination rates, reduced lipid peroxidation, and improved seedling growth. Our findings highlight that the targeted knockout of <i>LOX</i> genes, particularly the double mutation of <i>HvLOXA</i> and <i>HvLOXC1</i>, represents a promising genetic strategy for improving grain storability and nutritional value in barley.
DOI: 10.6084/m9.figshare.21572159
title: Misinformation in the media: global coverage of GMOs 2019-2021

description: Misinformation is a serious problem in scientific debates ranging from climate change to vaccines to nuclear power. In this study we quantitatively assess the phenomenon of misinformation – defined as information which is at variance with widely-accepted scientific consensus – on genetically modified crops and food (“GMOs”) in the mainstream and online news media over a two-year period. We found an overall falsehood rate of 9% with a potential readership of 256 million. None of the misinformation was positive in sentiment; most was negative. About a fifth of Africa’s media coverage on GMOs contained misinformation, a worrying finding given the potential for genetic engineering to deliver improved nutrition and food security in the continent. We conclude that misinformation about GMOs in the mainstream media is still a significant problem, and outranks the proportion of misinformation in other comparable debates such as COVID-19 and vaccines.
DOI: 10.6084/m9.figshare.21572159.v2
title: Misinformation in the media: global coverage of GMOs 2019-2021

description: Misinformation is a serious problem in scientific debates ranging from climate change to vaccines to nuclear power. In this study we quantitatively assess the phenomenon of misinformation – defined as information which is at variance with widely-accepted scientific consensus – on genetically modified crops and food (“GMOs”) in the mainstream and online news media over a two-year period. We found an overall falsehood rate of 9% with a potential readership of 256 million. None of the misinformation was positive in sentiment; most was negative. About a fifth of Africa’s media coverage on GMOs contained misinformation, a worrying finding given the potential for genetic engineering to deliver improved nutrition and food security in the continent. We conclude that misinformation about GMOs in the mainstream media is still a significant problem, and outranks the proportion of misinformation in other comparable debates such as COVID-19 and vaccines.
DOI: 10.6084/m9.figshare.28089854
title: <i>ZmNF-YB10</i>, a maize NF-Y transcription factor, positively regulates drought and salt stress response in <i>Arabidopsis thaliana</i>

description: Maize (<i>Zea mays</i> L.) is a major food and feed crop and an important raw material for energy, chemicals, and livestock. The NF-Y family of transcription factors in maize plays a crucial role in the regulation of plant development and response to environmental stress. In this study, we successfully cloned and characterized the maize NF-Y transcription factor gene <i>ZmNF-YB10</i>. We used bioinformatics, quantitative fluorescence PCR, and other techniques to analyze the basic properties of the gene, its tissue expression specificity, and its role in response to drought, salt, and other stresses. The results indicated that the gene was 1209 base pairs (bp) in length, with a coding sequence (CDS) region of 618 bp, encoding a polypeptide composed of 205 amino acid residues. This polypeptide has a theoretical isoelectric point of 5.85 and features a conserved structural domain unique to the NF-Y family. Quantitative fluorescence PCR results demonstrated that the <i>ZmNF-YB10</i> gene was differentially upregulated under drought and salt stress treatments but exhibited a negatively regulated expression pattern under alkali and cold stress treatments. Transgenic <i>Arabidopsis thaliana</i> subjected to drought and salt stress in soil showed greener leaves than wild-type <i>A. thaliana</i>. In addition, the overexpression lines showed reduced levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide (O<sup>2-</sup>), and malondialdehyde (MDA) and increased activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Western blot analysis revealed a distinct band at 21.8 kDa. Salt and drought tolerance analyses conducted in <i>E. coli</i> BL21 indicated a positive regulation. In yeast cells, <i>ZmNF-YB10</i> exhibited a biological function that enhances salt and drought tolerance. Protein interactions were observed among the <i>ZmNF-YB10</i>, <i>ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes. It is hypothesized that the <i>ZmNF-YB10, ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes may play a role in the response to abiotic stresses, such as drought and salt tolerance, in maize.
DOI: 10.6084/m9.figshare.28089854.v1
title: <i>ZmNF-YB10</i>, a maize NF-Y transcription factor, positively regulates drought and salt stress response in <i>Arabidopsis thaliana</i>

description: Maize (<i>Zea mays</i> L.) is a major food and feed crop and an important raw material for energy, chemicals, and livestock. The NF-Y family of transcription factors in maize plays a crucial role in the regulation of plant development and response to environmental stress. In this study, we successfully cloned and characterized the maize NF-Y transcription factor gene <i>ZmNF-YB10</i>. We used bioinformatics, quantitative fluorescence PCR, and other techniques to analyze the basic properties of the gene, its tissue expression specificity, and its role in response to drought, salt, and other stresses. The results indicated that the gene was 1209 base pairs (bp) in length, with a coding sequence (CDS) region of 618 bp, encoding a polypeptide composed of 205 amino acid residues. This polypeptide has a theoretical isoelectric point of 5.85 and features a conserved structural domain unique to the NF-Y family. Quantitative fluorescence PCR results demonstrated that the <i>ZmNF-YB10</i> gene was differentially upregulated under drought and salt stress treatments but exhibited a negatively regulated expression pattern under alkali and cold stress treatments. Transgenic <i>Arabidopsis thaliana</i> subjected to drought and salt stress in soil showed greener leaves than wild-type <i>A. thaliana</i>. In addition, the overexpression lines showed reduced levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide (O<sup>2-</sup>), and malondialdehyde (MDA) and increased activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Western blot analysis revealed a distinct band at 21.8 kDa. Salt and drought tolerance analyses conducted in <i>E. coli</i> BL21 indicated a positive regulation. In yeast cells, <i>ZmNF-YB10</i> exhibited a biological function that enhances salt and drought tolerance. Protein interactions were observed among the <i>ZmNF-YB10</i>, <i>ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes. It is hypothesized that the <i>ZmNF-YB10, ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes may play a role in the response to abiotic stresses, such as drought and salt tolerance, in maize.
DOI: 10.6084/m9.figshare.29332692
title: Metabolomic response of <i>Zizania latifolia</i> to low-temperature stress and identification of the bZIP transcription factor family

description: Cold stress severely impacts crop production, making it crucial to dissect the metabolic and transcriptional regulatory mechanisms of cold-resistant plants for breeding cold-tolerant varieties. This study systematically explored the response mechanism of <i>Zizania latifolia</i> to cold stress by integrating widely targeted metabolomics and genome-wide analysis for the first time. Metabolomics analysis revealed that 690 out of 810 metabolites showed significant differences after cold treatment at 4°C, with significant enrichment of flavonoids, amino acid derivatives, and alkaloids, involving key pathways such as antioxidant defense, osmotic adjustment, and signal transduction. This indicates that <i>Z. latifolia</i> copes with cold stress through the coordination of secondary and primary metabolism. A total of 115 bZIP transcription factors (ZlbZIPs) were identified from the <i>Z. latifolia</i> genome, with 18 genes located in known cold-resistant quantitative trait locus (QTL) intervals. Four cold-tolerant candidate genes were screened through collinearity analysis with the rice genome. Expression analysis showed that <i>ZlbZIP005, ZlbZIP075</i>, and <i>ZlbZIP084</i> were significantly upregulated (29.17–4.10 fold) at 24 hours of cold treatment, and their promoter regions with high-density G-box elements implied strong cold response potential. Phylogenetic and evolutionary analyses showed that the bZIP family of <i>Z. latifolia</i> is highly homologous to that of rice but exhibits subfamily-specific expansion (such as subfamily Ⅶ) and conserved motif variations related to functional differentiation. This study first elucidated the metabolic reprogramming and bZIP transcription factor regulatory network of <i>Z. latifolia</i> under cold stress. The screened key cold-tolerant genes provide important genetic resources for cold-resistant breeding of gramineous crops and lay a foundation for analyzing the molecular mechanism of plant cold resistance and genetic improvement.
DOI: 10.6084/m9.figshare.29332692.v1
title: Metabolomic response of <i>Zizania latifolia</i> to low-temperature stress and identification of the bZIP transcription factor family

description: Cold stress severely impacts crop production, making it crucial to dissect the metabolic and transcriptional regulatory mechanisms of cold-resistant plants for breeding cold-tolerant varieties. This study systematically explored the response mechanism of <i>Zizania latifolia</i> to cold stress by integrating widely targeted metabolomics and genome-wide analysis for the first time. Metabolomics analysis revealed that 690 out of 810 metabolites showed significant differences after cold treatment at 4°C, with significant enrichment of flavonoids, amino acid derivatives, and alkaloids, involving key pathways such as antioxidant defense, osmotic adjustment, and signal transduction. This indicates that <i>Z. latifolia</i> copes with cold stress through the coordination of secondary and primary metabolism. A total of 115 bZIP transcription factors (ZlbZIPs) were identified from the <i>Z. latifolia</i> genome, with 18 genes located in known cold-resistant quantitative trait locus (QTL) intervals. Four cold-tolerant candidate genes were screened through collinearity analysis with the rice genome. Expression analysis showed that <i>ZlbZIP005, ZlbZIP075</i>, and <i>ZlbZIP084</i> were significantly upregulated (29.17–4.10 fold) at 24 hours of cold treatment, and their promoter regions with high-density G-box elements implied strong cold response potential. Phylogenetic and evolutionary analyses showed that the bZIP family of <i>Z. latifolia</i> is highly homologous to that of rice but exhibits subfamily-specific expansion (such as subfamily Ⅶ) and conserved motif variations related to functional differentiation. This study first elucidated the metabolic reprogramming and bZIP transcription factor regulatory network of <i>Z. latifolia</i> under cold stress. The screened key cold-tolerant genes provide important genetic resources for cold-resistant breeding of gramineous crops and lay a foundation for analyzing the molecular mechanism of plant cold resistance and genetic improvement.
DOI: 10.6084/m9.figshare.29979713
title: Genome-wide profiling of GRAS genes in flax (<i>Linum usitatissimum</i> L.) reveals <i>LuGRAS30</i> as a key regulator of drought stress resistance

description: GRAS genes are indispensable for modulating plant growth, developmental patterning, and adaptive responses to biotic and abiotic stress conditions. In this study, 99 <i>LuGRAS</i> genes were identified in the flax genome. Phylogenetic analysis classified them into 10 subfamilies: HAM, DELLA, DLT, SCL3, LAS, SCL4/7, SCR, SCL, SHR, and PAT1. Gene structure and motif analyses revealed that <i>LuGRAS</i> genes within the same clade exhibited conserved exon-intron organization and motif architectures. Promoter analysis showed that most <i>LuGRAS</i> genes contained cis-regulatory elements responsive to plant hormones (MeJA and abscisic acid) and abiotic stresses, including anaerobic induction, low temperature, and drought inducibility. MiRNA target prediction indicated that lus-miR395 is the primary regulatory miRNA for the <i>LuGRAS</i> gene family. Expression pattern analysis demonstrated that all <i>LuGRAS</i> family members were highly expressed in leaves and roots. qRT-PCR analysis further revealed that 10 genes were significantly upregulated under abiotic stresses (cold, drought, and salt), suggesting their involvement in antioxidant defense mechanisms. In <i>Arabidopsis</i>, <i>LuGRAS30</i> enhanced drought tolerance by scavenging reactive oxygen species (ROS) accumulation. Subcellular localization analysis demonstrated that <i>LuGRAS30</i> was localized in the nucleus. This study provides new insights into the role of <i>LuGRAS</i> genes in flax stress tolerance and contributes to flax breeding and further functional research.
DOI: 10.6084/m9.figshare.29979713.v1
title: Genome-wide profiling of GRAS genes in flax (<i>Linum usitatissimum</i> L.) reveals <i>LuGRAS30</i> as a key regulator of drought stress resistance

description: GRAS genes are indispensable for modulating plant growth, developmental patterning, and adaptive responses to biotic and abiotic stress conditions. In this study, 99 <i>LuGRAS</i> genes were identified in the flax genome. Phylogenetic analysis classified them into 10 subfamilies: HAM, DELLA, DLT, SCL3, LAS, SCL4/7, SCR, SCL, SHR, and PAT1. Gene structure and motif analyses revealed that <i>LuGRAS</i> genes within the same clade exhibited conserved exon-intron organization and motif architectures. Promoter analysis showed that most <i>LuGRAS</i> genes contained cis-regulatory elements responsive to plant hormones (MeJA and abscisic acid) and abiotic stresses, including anaerobic induction, low temperature, and drought inducibility. MiRNA target prediction indicated that lus-miR395 is the primary regulatory miRNA for the <i>LuGRAS</i> gene family. Expression pattern analysis demonstrated that all <i>LuGRAS</i> family members were highly expressed in leaves and roots. qRT-PCR analysis further revealed that 10 genes were significantly upregulated under abiotic stresses (cold, drought, and salt), suggesting their involvement in antioxidant defense mechanisms. In <i>Arabidopsis</i>, <i>LuGRAS30</i> enhanced drought tolerance by scavenging reactive oxygen species (ROS) accumulation. Subcellular localization analysis demonstrated that <i>LuGRAS30</i> was localized in the nucleus. This study provides new insights into the role of <i>LuGRAS</i> genes in flax stress tolerance and contributes to flax breeding and further functional research.
DOI: 10.15468/zfpc6y
title: Validation of the designation Aliinostoc bakau Merican & al. (Nodulariaceae, Cyanobacteria)

description: This dataset contains the digitized treatments in Plazi based on the original journal article Merican, Faradina, School, Narongrit Muangmai (2024): Validation of the designation Aliinostoc bakau Merican & al. (Nodulariaceae, Cyanobacteria). Notulae Algarum 312: 1-2, DOI: 10.5281/zenodo.16652797