cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0kw5f73
title: CIL:12952, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to GOLPH3 (rabbit polyclonal GMx33) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (10 nm anti-rabbit, 15 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 21,000 X. See Ladinsky and Howell (2007) for more information on methods used.
description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and GOLPH3 (a trans-Golgi associated protein that can also be associated with mitochondria; smaller gold particles) staining. Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
DOI: 10.6075/j0br8rdn
title: CIL:1295, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
DOI: 10.6075/j0t152qc
title: CIL:12945, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 21,000 X. See Ladinsky and Howell (2007) for more information on methods used.
description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
DOI: 10.6075/j01j98zj
title: CIL:12947, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 15,500 X. See Ladinsky and Howell (2007) for more information on methods used.
description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
DOI: 10.6075/j0p84b1h
title: CIL:12944, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 21,000 X. See Ladinsky and Howell (2007) for more information on methods used.
DOI: 10.6075/j0h41q5s
title: CIL:12016, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library

description: Local application of calyculin A (phosphatase inhibitor) can stimulate motility initiation. Phase-contrast time-lapse video of a stationary keratocyte exposed to a local application of calyculin A. Red pseudocolor indicates the calyculin A flow from the micropipette (positioned in the bottom left corner). The keratocyte polarized and initiated motility away from the source of calyculin A. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Corresponds to Fig. 6 C and video 7 in JCB 178:1207-1221, 2007.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0ff3rkt
title: CIL:1197, Caenorhabditis elegans, muscle cell. In Cell Image Library

description: The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 4 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin
Processing history: unprocessed raw data
description: C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.
DOI: 10.6075/j0bc3x87
title: CIL:11963, Arbacia punctulata, embryonic cell. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: The movie illustrates two approaches to understanding the 3D structure of complex objects, using the pluteus stage embryo of the sea urchin Arbacia punctulata. The first sequence is a through-focus DIC series presented as a 'walk through' in the z dimension. The second uses projections at different angles through a z-stack, with the results presented as a 'rocking' series in which the 3D structure becomes more readily apparent.
description: See: K Okazaki and S Inoue (1976). Crystal properties of the larval sea urchin spicule. Dev Growth Diff 18:413-434; S Inoue and T Inoue (1986) Computer-aided stereomicroscopic video reconstruction and serial display from high-resolution light microscope optical sections. Ann NY Acad Sci 483:392-404.
DOI: 10.6075/j06m35kb
title: CIL:11970, unidentified spirochete, Hypermastigote protozoan. In Cell Image Library

description: The movie shows the wave-like effect of compensated birefringence caused by rotating spirochetes attached to the surface of a hypermastigote protozoan. The image was obtained using a rectified polarizing microscope with compensator.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed
DOI: 10.6075/j0t43rt8
title: CIL:11972, Tetrahymena pyriformis. In Cell Image Library

description: Preparation of the live ciliate Tetrahymena pyriformis stained with a mitochondria-specific probe (Mitotracker green) and viewed with the CSU-10 confocal microscope. The mitochondria become visible as they swim through the confocal plane of focus. Non-confocal background light was superimposed by backlighting through the condenser to make the whole organism visible.
description: See: S. Inoue and T. Inoue (2002) Direct view high-speed confocal scanner: the CSU-10. Meth Cell Biol 70:87-127.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: probe for mitochondria
Data qualification: Processed
DOI: 10.6075/j0m907bh
title: CIL:11969, Homo sapiens, erythrocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: See: J F Hoffman and S Inoue (2006). Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human and Amphiuma red blood cells. Proc Natl Acad Sci USA 103:2971-2976.
description: The movie shows the behaviour of human erythrocytes suspended in a percoll gradient and exposed to centrifugal force in the centrifuge polarizing microscope developed by S. Inoue. Here, the contrast is generated by differential interference contrast (DIC). Erythrocytes are distorted in shape in the centrifugal field, the hemoglobin collecting at the lower part of the cell causing it to bulge. The movie shows the additions of single cells to a stack of cells (rouleaux). Upon joining a rouleaux, the erythrocytes instantly recover their normal undistorted biconcave shape.
DOI: 10.15468/dl.5zc875
title: Occurrence Download

description: A dataset containing 332522 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Rhus typhina, Parthenocissus quinquefolia, Spiraea billardii, Prunus laurocerasus, Rosa rugosa, Spiraea alba, Pistia stratiotes, Solidago gigantea, Rubus armeniacus, Robinia pseudoacacia, Acer negundo, Ailanthus altissima, Alternanthera philoxeroides, Aponogeton distachyos, Arundo donax, Asclepias syriaca, Azolla filiculoides, Baccharis halimifolia, Cabomba caroliniana, Carpobrotus, Cotoneaster horizontalis, Egeria densa, Eichhornia crassipes, Elaeagnus angustifolia, Elodea canadensis, Elodea nuttallii, Fallopia baldschuanica, Fraxinus pennsylvanica, Gymnocoronis spilanthoides, Helianthus tuberosus, Helianthus laetiflorus, Heracleum mantegazzianum, Hydrilla verticillata, Hydrocotyle ranunculoides, Impatiens capensis, Impatiens glandulifera, Koenigia polystachya, Laburnum anagyroides, Lagarosiphon major, Lemna minuta, Lemna turionifera, Limnobium laevigatum, Reynoutria japonica, Myriophyllum rubricaule, Prunus serotina, Parthenocissus tricuspidata, Mahonia aquifolium, Parthenocissus inserta, Spiraea tomentosa, Spiraea douglasii, Reynoutria bohemica, Salvinia molesta, Myriophyllum heterophyllum, Rudbeckia laciniata, Ludwigia grandiflora, Reynoutria sachalinensis, Spiraea salicifolia, Solidago canadensis, Myriophyllum aquaticum, Ludwigia peploides, Zizania latifolia)",
 "Country is Belgium",
 "Year is greater than or equal to 2013",
 "HasCoordinate is true",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 332522 records from 42 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009596-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j02j69k6
title: CIL:12013, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library

description: Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry and initiating motility. The cell has been reoriented such that the eventual direction of movement is to the right. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Corresponds to Fig. 2 A (cell B) and video 4 in JCB 178:1207-1221, 2007.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0h70fv3
title: CIL:12008, Danio rerio, mucus secreting cell, immune cell. In Cell Image Library

description: Preparation: living tissue
Item type: fluorescence microscopy
Imaging mode: living tissue
description: A polymorphic immune cell (red) glides over two spherical V12Ras-transformed mucus-secreting cells (green, possible tumor precursors) in a live three day old zebrafish embryo. This is one of four images in a series that illustrates the movement of the immune cell over the surface of the transformed mucus-secreting cells.
DOI: 10.6075/j09c6wqg
title: CIL:120, Gallus gallus gallus, erythrocyte. In Cell Image Library

description: Telomeres isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. 
 Image recorded with FEI Tecnai 12 TEM at 100KV and recorded on 2048x2048 CCD with 2x binning.
 Telomeres have a looped conformation, which likely aids in their protection from the rapid degradation that occurs with free DNA ends.
description: Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons; charge coupled device (CCD)
Parameter imaged: electron density
Source of contrast: elastic scattering
Visualization methods: uranyl salt; negative staining
Processing history: unprocessed raw image
DOI: 10.6075/j0833qs3
title: CIL:11973, Thyone briareus, sperm. In Cell Image Library

description: The movie shows, in real time, the explosive acrosomal reaction of Thyone (sea cucumber) sperm. The field shows several sperm heads (bright contrast) with tails of some in the plane of focus. From the head at lower left, a thin thread, resulting from the rapid polymerization of actin, is extruded. The acrosomal process is thought to contribute to fertilization by penetrating the jelly coat of the egg.
description: See: S Inoue and LG Tilney (1982) Acrosomal reaction of Thyone sperm I J Cell Biol 93:812-819;
LG Tilney and S Inoue (1982) Acrosomal reaction of Thyone sperm II J Cell Biol 93:820-827; LG Tilney and S Inoue (1985) Acrosomal reaction of Thyone sperm III J Cell Biol 100:820-1273-1283.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Data qualification: Processed