DOI: 10.5061/dryad.bvq83bkkp
title: The facultative intracellular symbiont Lariskella is neutral for lifetime fitness and spreads through cytoplasmic incompatibility in the leaffooted bug, Leptoglossus zonatus

description: To examine the effects of the intracellular symbiont
 Lariskella on bug performance and reproduction as well as in
 possible interactions with the bug’s obligate nutritional
 symbiont, Caballeronia, Leptoglossus zonatus individuals were
 reared in a factorial experiment with
 both Lariskella and Caballeronia positive and negative treatments. Bug performance was measured based on lifetime survival analysis, development time, and weight. Lifetime reproductive success was measured based on clutch size and egg hatch rate of adult female L. zonatus with and without Lariskella. In a second experiment, we evaluated egg mortality in reproductive crosses with and without Lariskella (in all four possible combinations) to evaluate the possibility that Lariskella caused cytoplasmic incompatibility. Finally, we sampled bugs from three L. zonatus populations in USA on pomegranates: two populations in California (Bakersfield and Fresno) and one from Tucson, Arizona to determine Lariskella infection frequency in the field.
description: This README file was generated on 2025-01-25 by Edwin F. Umanzor
 \##################### General information ########################### 1.
 Title of Dataset: The facultative intracellular symbiont Lariskella is
 neutral for lifetime fitness and spreads through cytoplasmic
 incompatibility in the leaffooted bug, Leptoglossus zonatus 2. Author
 Information A. Principal Investigator Contact Information Name: Martha S.
 Hunter Institution: The University of Arizona Address: Tucson, AZ USA
 Email: [mhunter@ag.arizona.edu](mailto:mhunter@ag.arizona.edu) B. Lead
 Author Contact Information Name: Edwin F. Umanzor Institution: The
 University of Arizona Address: Tucson, AZ USA Email:
 [Edwinnzor@arizona.edu](mailto:Edwinnzor@arizona.edu) 3. Date of data
 collection: 2018-2024 4. Geographic location of data collection:
 Bakersfield (CA), Fresno (CA), Tucson (AZ) \##################### Data
 & File Overview ########################### 1. File List: A)
 Lariskella_development_titer.csv B)
 Lariskella_reproductive_tissue_titer.csv C) LxB_mortality_long.csv D)
 LxB_development_long.csv E) LxB_adult_weight_excluded_neg_PCR.csv F)
 zonatus_egg_data_updated.csv G) zonatus_CI_eggcounts_C2.csv H)
 Lariskella_survey_2018-2019.csv I) Temporal_survey_lariskella_screen.csv
 \##################### Data specific information
 ########################### A) Lariskella_development_titer.csv 1. Number
 of variables: 6 2. Number of cases/rows: 26 3. Variable List: * stage: bug
 developmental stage * sample: sample identifier * volume(ul): total DNA
 extraction volume * copy/2ul: copy number per 2ul used in qPCR * copy/ul:
 copy number per 1ul * copynum: total copy number based on total extraction
 volume 4. Missing data codes: None 5. Specialized formats or other
 abbreviations used: None
 \#########################################################################
 B) Lariskella_reproductive_tissue_titer.csv 1. Number of variables: 8 2.
 Number of cases/rows: 17 3. Variable List: *tissue: reproductive tissye
 sampled *sample: sample identifier *concentration(ng/ul): DNA
 concentration *volume(ul): total DNA extraction volume *totalDNA(ng):
 Total amount of DNA extracted *copy/4ng: copy number per 4ng of DNA
 *copy/ng: copy number per 1ng of DNA *copynum: total copy number based on
 total DNA extracted 4. Missing data codes: None 5. Specialized formats or
 other abbreviations used: None
 \#########################################################################
 C) LxB_mortality_long.csv 1. Number of variables: 8 2. Number of
 cases/rows: 203 3. Variable List: *start: observation start date *ddays:
 date of bug death or end of observation *box: bug rearing box identifier
 *id: bug identifier *treatment: bug symbiont infection status:
 L-(Lariskella negative), L+(Lariskella positive), B-(Caballeronia
 negative), B+(Caballeronia positive) *dayss: number of days survived or
 total observation time *stage: bug development stage at time of death or
 end of observation *status: 1= bug observed until it died, 0= observation
 interrupted 4. Missing data codes: None 5. Specialized formats or other
 abbreviations used: None
 \#########################################################################
 D) LxB_development_long.csv 1. Number of variables: 8 2. Number of
 cases/rows: 850 3. Variable List: *start: hatch date *obsdate: date when
 the next developmental stage was observed in the rearing box *id: bug
 rearing box identifier *treatment: bug symbiont infection status:
 L-(lariskella negative), L+(lariskella positive), B-(Caballeronia
 negative), B+(Caballeronia positive) *days: number of days from hatch to
 observed developmental stage *inocdate: date when Caballeronia was fed to
 2nd instar bugs *pop: bug developmental stage *count: total number of bugs
 of the specified developmental stage 4. Missing data codes: NA = not
 available, "count" variable has missing cells and kept empty to
 avoid any potential conflicts with R code. Number entered represents all
 bugs of the specific developmental stage, and empty cells = number entered
 - 1 5. Specialized formats or other abbreviations used: None
 \#########################################################################
 E) LxB_adult_weight_excluded_neg_PCR.csv 1. Number of variables: 5 2.
 Number of cases/rows: 86 3. Variable List: *box: bug rearing box
 identifier *treatment: bug symbiont infection status: L-(lariskella
 negative), L+(lariskella positive), B-(Caballeronia negative),
 B+(Caballeronia positive) *sex: bug sex (M=male, F=female) *id: bug
 identifier *weight(g): bug weight in grams 4. Missing data codes: None 5.
 Specialized formats or other abbreviations used: None
 \#########################################################################
 F) zonatus_egg_data_updated.csv 1. Number of variables: 16 2. Number of
 cases/rows: 262 3. Variable List: *pairdate: cross start date *ovidate:
 oviposition date *p1: rearing box identifier of 1st individual in the
 cross *p1sex: sex of first individual in a cross *p1id: bug identifier of
 first individual in the cross *p2: rearing box identifier of 2nd
 individual in the cross *p2sex: sex of second individual in a cross *p2id:
 bug identifier of second individual in the cross *treatment: bug symbiont
 infection status: L-(lariskella negative), L+(lariskella positive),
 B-(Caballeronia negative), B+(Caballeronia positive) *cross: both
 individuals in the cross *hdate: egg hatch date *days: number of days from
 pairing to hatching *teggs: total number of eggs in clutch *heggs: total
 number of eggs that hatched *reggs: total number of eggs that failed to
 hatch *dde: total number of nymphs that died during hatching 4. Missing
 data codes: NA = not available 5. Specialized formats or other
 abbreviations used: None
 \#########################################################################
 G) zonatus_CI_eggcounts_C2.csv 1. Number of variables: 11 2. Number of
 cases/rows: 46 3. Variable List: *odate: oviposition date *cross:
 infection status of both individual in the cross *tegg: total number of
 eggs in clutch *regg: total number of eggs with a normal chorion and a
 fully formed embryo inside *hatch: percentage of "tegg"s that
 are classified as "regg"s *fegg: total number of c1 and c2 eggs
 *dde: total number of nymphs that died during hatching *c1: total number
 of eggs that failed to hatch but have a normal colored chorion and a fully
 formed embryo inside *c2: total number of eggs that failed to hatch but
 have a transluscent chorion and lacks a fully formed embryo inside *id:
 rearing box identifier *hdate: egg hatch date 4. Missing data codes: None
 5. Specialized formats or other abbreviations used: L-(lariskella
 negative), L+(lariskella positive), B-(Caballeronia negative),
 B+(Caballeronia positive), M=male, F=female
 \#########################################################################
 H) Lariskella_survey_2018-2019.csv 1. Number of variables: 5 2. Number of
 cases/rows: 248 3. Variable List: *extractionID: DNA extraction identifier
 *bugid: bug identifier *collectiondate: bug collection date *region: bug
 collection region *results: Lariskella PCR results (pos=positive,
 neg=negative) 4. Missing data codes: None 5. Specialized formats or other
 abbreviations used: None
 \#########################################################################
 I) Temporal_survey_lariskella_screen.csv 1. Number of variables: 2 2.
 Number of cases/rows: 148 3. Variable List: * Date: bug collection date *
 infection: Lariskella PCR results (pos=positive, neg=negative) 4. Missing
 data codes: None 5. Specialized formats or other abbreviations used: None