DOI: 10.5061/dryad.31zcrjf0k
title: Data from: Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the colon

description: Interstitial cells of Cajal (ICC) generate contractile patterns of colonic
 motility. We investigated innervation of ICC within the plane of the
 myenteric plexus (ICC-MY) in proximal colon using mice expressing GCaMP6f
 in ICC. ICC-MY generated localized Ca2+ transients that couple to
 activation of ANO1 channels, a Ca2+-activated Cl- conductance. ICC are
 electrically coupled to SMCs, so activation or suppression of currents in
 ICC affects excitability of SMCs. ICC-MY displayed tonic inhibition, as
 the neurotoxin, TTX, increased the frequency of Ca2+ transients. Tonic
 inhibition was mimicked by a nitric oxide donor, NONOate, and by a
 guanylate cyclase agonist (Bay 58-2667). In contrast, ODQ mimicked the
 effects of TTX, increasing Ca2+ transients. Carbachol (CCh) increased Ca2+
 transients in ICC-MY, and these effects were mediated by M3 muscarinic
 receptors. Neostigmine also increased Ca2+ transients, suggesting there is
 tonic activation of enteric excitatory neurons in colonic muscles.
 Substance P and antagonists of NK1 and NK2 receptors had no effect on Ca2+
 transients in ICC-MY. Electrical field stimulation (EFS), under conditions
 that emphasized excitatory neural responses, enhanced Ca2+ transients, and
 these effects were blocked by atropine or an M3 receptor antagonist (DAU
 5884). EFS in the presence of atropine caused inhibition of Ca2+ via
 release of NO. Cessation of nitrergic stimulation resulted in a
 substantial increase in Ca2+ transients, known as post-stimulus
 excitation. In summary, ICC-MY, important for the generation of propulsive
 contractions in the colon, are innervated by excitatory (cholinergic) and
 inhibitory (nitrergic) motor neurons, and these inputs regulate the
 excitability of these cells.
description: # Data from: Neuronal regulation of myenteric interstitial cells of Cajal
 (ICC-MY) in the colon Dataset DOI:
 [10.5061/dryad.31zcrjf0k](10.5061/dryad.31zcrjf0k) ## Description of the
 data and file structure The file includes image datasets from ICC-MY
 cellular calcium events under control and pharmacological agents. This
 directory contains subfolders related to different compounds used in
 experiments. Each folder contains data corresponding to a specific control
 subfolder and treatment subfolder. Below is a brief description of each
 subfolder: ### Files and variables #### File: Bay58.zip
 **Description:** Contains experimental data related to Bay-58-2667 and its
 effects on cellular activity.   2 subfolders: control and in the presence
 of Bay-58-2667 #### File: CCH.zip **Description:** Includes data for
 Carbachol (CCH) affecting calcium signaling in ICC-MY.   2 subfolders:
 Control and in the presence of CCH #### File: MEN-10376.zip
 **Description:** Contains data for experiments using MEN-10376 and its
 effects on ICC-MY calcium transients.   2 subfolders: Control and in the
 presence of MEN-10376 #### File: Neostigmine.zip **Description:** Stores
 experimental results related to Neostigmine on ICC-MY calcium signaling.  
 2 subfolders: Control and in the presence of Neostigmine #### File:
 Nononate.zip **Description:** A subset of Nononate experiments effects on
 ICC-MY calcium signals.   2 subfolders: control and in the presence of
 Nononate #### File: ODQ.zip **Description:** Contains data related to ODQ,
 effects on ICC-MY calcium signals.   2 subfolders: control and in the
 presence of ODQ #### File: RP-67580.zip **Description:** Includes
 experimental data involving RP-67580, effects on ICC-MY calcium dynamics.
   2 subfolders: control and in the presence of RP-67580 #### File:
 SubstanceP.zip **Description:** Contains results from experiments with
 Substance P, effects on ICC-MY calcium homeostasis.   2 subfolders:
 control and in the presence of Substance P #### File: TTX.zip
 **Description:** Contains results from experiments with TTX, effects on
 ICC-MY calcium transients.   2 subfolders: control and in the presence of
 Substance P   ## Code/software * Fiji/ImageJ (National Institutes of
 Health, MD, USA, [http://rsbweb.nih.gov/ij](http://rsbweb.nih.gov/ij)). *
 STMapAuto
 software: [https://github.com/gdelvalle99/STMapAuto](https://github.com/gdelvalle99/STMapAuto)