DOI: 10.6084/m9.figshare.31359796
title: Morphometric Analysis of Adult (P30) Ventricular Cardiomyocytes Following Cardiomyocyte-Specific PKN2 Deletion

description: This file contains single-cell morphometric measurements of isolated adult ventricular cardiomyocytes at postnatal day 30 (P30). Each row corresponds to one segmented cardiomyocyte and includes sample identifiers (animal and group), image/file provenance, CellProfiler-derived shape features (area, major/minor axis, eccentricity, Feret diameters, radius metrics), and calibrated size readouts in microns (Area_micron, MajorAxis_micron, MinorAxis_micron).<b>Identifiers / indexing</b><b>Unnamed: 0</b>: CSV export row index (not biological).<b>ImageNumber</b>: CellProfiler image ID used to segment this cell.<b>ObjectNumber</b>: CellProfiler object ID within <i>ImageNumber</i>.<b>QC / classification</b><b>class</b>: CellProfiler Analyst class label (used to keep valid CMs and exclude artifacts/hypercontracted cells).<b>class_number</b>: Numeric encoding of <i>class</i>.<b>Sample & acquisition metadata</b><b>Metadata_Animal</b>: Animal ID (biological replicate) from image metadata.<b>Animal</b>: Cleaned/standardized animal ID used for analysis/modeling.<b>Group</b>: Experimental group (e.g., Control vs cKO).<b>Metadata_type</b>: Condition/type label embedded in metadata (often overlaps with <i>Group</i> or genotype label).<b>Metadata_Channel</b>: Imaging channel identifier used for segmentation/measurement.<b>Metadata_FileLocation</b>: Source file location string from acquisition/processing.<b>Metadata_Frame</b>: Frame index (if multi-frame/time series).<b>Metadata_Series</b>: Series index (if multi-series acquisition).<b>File provenance (traceability to raw images)</b><b>FileName_Cells</b>: Image filename used for cell segmentation.<b>FileName_Nucleus</b>: Image filename for nuclear channel (if present/linked).<b>PathName_Cells</b>: Path to the cell image.<b>PathName_Nucleus</b>: Path to the nuclear image.<b>Morphometrics (CellProfiler </b><b><i>AreaShape_</i></b><b>; typically in pixel units unless otherwise stated)</b><b>AreaShape_Area</b>: Segmented cell area.<b>AreaShape_BoundingBoxArea</b>: Area of minimal bounding rectangle around the cell.<b>AreaShape_Compactness</b>: Compactness/shape irregularity metric (higher = less compact/more irregular).<b>AreaShape_ConvexArea</b>: Area of the convex hull of the cell.<b>AreaShape_Eccentricity</b>: Ellipse eccentricity (0=circle, 1=highly elongated).<b>AreaShape_MajorAxisLength</b>: Major axis length of best-fit ellipse (cell “length”).<b>AreaShape_MinorAxisLength</b>: Minor axis length of best-fit ellipse (cell “thickness/width”).<b>AreaShape_MaxFeretDiameter</b>: Maximum Feret (caliper) diameter.<b>AreaShape_MinFeretDiameter</b>: Minimum Feret (caliper) diameter.<b>AreaShape_MaximumRadius</b>: Max centroid-to-boundary distance.<b>AreaShape_MeanRadius</b>: Mean centroid-to-boundary distance.<b>AreaShape_MedianRadius</b>: Median centroid-to-boundary distance.<b>Calibrated outputs (micron units)</b><b>Area_micron</b>: Cell area in µm² (calibrated from <i>AreaShape_Area</i>).<b>MajorAxis_micron</b>: Cell length in µm (calibrated from <i>AreaShape_MajorAxisLength</i>).<b>MinorAxis_micron</b>: Cell thickness/width in µm (calibrated from <i>AreaShape_MinorAxisLength</i>).