DOI: 10.5061/dryad.zkh1893kv
title: Data from: Role of Atg3, Atg5, and Atg12 in the crosstalk between apoptosis and autophagy in the posterior silk gland of Bombyx mori

description: Autophagy is a cellular mechanism that enhances cell survival in response
 to various stressors, including nutrient deprivation; however, it also
 plays a pivotal role in the regulation of programmed cell death. This
 study examined the effects of autophagy-related genes Atg3, Atg5, and
 Atg12 on apoptosis and autophagy during the degeneration of the posterior
 silk gland in Bombyx mori, employing RNA interference techniques.
 Apoptosis-specific markers and autophagic processes were evaluated in both
 control and treatment groups. The knockdown of all three genes resulted in
 a significant reduction in autophagy, modifications in the apoptosis
 process, aberrant expression of p53, and impaired lysosomal function. It
 was determined that Atg3 is involved in the regulation of intracellular
 mitochondrial homeostasis. Following the silencing of Atg5, evidence was
 obtained indicating the gene's role in regulating lysosomal pH.
 Notably, the loss of Atg3 and Atg5 was associated with an increase in
 apoptotic markers, whereas the silencing of Atg12 inhibited apoptosis.
 Elevated levels of the p53 transcription factor following gene silencing
 suggested a potential interaction between these genes and p53. Our
 findings further underscore the importance of autophagy-mediated cell
 death, involving Atg3, Atg5, and Atg12, in the proper progression of
 degeneration in the posterior silk gland. A comprehensive understanding of
 the molecular mechanisms that mediate the interaction between apoptosis
 and autophagy is essential for elucidating their roles in both
 physiological and pathological contexts.
description: ## Role of Atg3, Atg5, and Atg12 in the crosstalk between apoptosis and
 autophagy in the posterior silk gland of Bombyx mori
 [https://doi.org/10.5061/dryad.zkh1893kv](https://doi.org/10.5061/dryad.zkh1893kv) ## Description of the data and file structure **File: RAW_DATA.xlsx** **Description**: * The **Figure 1A tab** within the Excel file presents the data derived from quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis conducted on both control and treatment groups following the silencing of the Atg3, Atg5, Atg6, and Atg12 genes in Bombyx mori. - The tab labeled **Figure 2B tab** in the Excel file presents data analyzed through quantitative reverse transcription polymerase chain reaction (qRT-PCR) from both the control and gene-silencing groups. This analysis aims to assess the expression levels of autophagy-related genes, specifically Atg3, Atg5, Atg6, Atg8, and Atg12. * In the Excel file, **Figure 3A tab** contains data on the spectrophotometric analysis of acid phosphatase, a lysosomal enzyme, in the control and treatment groups. - In the Excel file, **Figure 4B tab** labeled contains the data derived from the analysis of TUNEL images obtained from both the control and gene-silenced groups using ImageJ software. * The **Figure 5A tab** in the Excel file presents the data acquired from the Western blot analysis of nuclear and cytoplasmic p53, cytosolic cytochrome c, and cleaved caspase-3 proteins in both control and gene-silenced groups. - All the missing data given as n/a.