DOI: 10.5061/dryad.6mh29
title: Data from: Cryptic diversity in the well-studied terrestrial worm Cognettia sphagnetorum (Clitellata: Enchytraeidae)

description: The terrestrial worm Cognettia sphagnetorum has been used as a model in
 several studies focusing on research areas such as climate change as well
 as forest and soil ecology; it has also been shown to play a key role in
 the decomposition of organic matter and nutrient cycling. Cognettia is an
 enchytraeid genus commonly found in acidic terrestrial habitats, such as
 coniferous forests and bogs. In this study, the diversity of the genus,
 with particular focus on the morphospecies C. sphagnetorum in northern
 Europe, is assessed using four molecular markers, the mitochondrial COI
 (cytochrome c oxidase subunit I) and16S (16S ribosomal RNA), and the
 nuclear H3 (Histone 3) and ITS (Internal Transcribed Spacer). The datasets
 were first delimited into Molecular Operational Units (MOTUs) and the
 existence of global barcoding-gaps was tested. Single gene-trees were then
 estimated for all genes using Bayesian Inference, and a species tree was
 estimated with all markers combined using the multi-species coalescence.
 The results show that in northern Europe the genus consists of at least
 eight MOTUs supported by all genes except H3. Four of these MOTUs were
 within the morphotaxon C. sphagnetorum and two within Cognettia
 glandulosa. C. sphagnetorum s.l. was found to be non-monophyletic in all
 gene-trees, as well as in the species tree. As the MOTUs were well
 separated and non-monophyly was observed within C. sphagnetorum s.l., we
 conclude that the MOTUs are best treated as separate species. Given that
 cryptic diversity was found in this genus, we recommend that material of
 Cognettia used in future studies should be identified using molecular
 barcodes.
description: log file from *BEAST
 analysisStarBEASTLog.log.txttree file from *BEAST analysisStarBEASTLog.species.treesxml input file for *BEAST analysisStarBEASTLog.xmlCognettia 16S consensus tree file from MrBayes analysis16S_CognettiaMAFFT.nex.conCognettia COI consensus tree file from MrBayes analysisCOI_cognettia.nex.conCognettia H3 consensus tree file from MrBayes analysisH3_Congettia.nex.conCognettia ITS consensus tree file from MrBayes analysisITS_CognettiaMAFFT.nex.conCognettia 16S MCMC file from MrBayes analysis16S_CognettiaMAFFT.nex.mcmcCognettia COI MCMC file from MrBayes analysisCOI_cognettia.nex.mcmcCognettia H3 MCMC file from MrBayes analysisH3_Congettia.nex.mcmcCognettia ITS MCMC file from MrBayes analysisITS_CognettiaMAFFT.nex.mcmcCognettia 16S nexus file for MrBayes analysis16S_CognettiaMAFFT.nexCognettia COI nexus file for MrBayes analysisCOI_Cognettia.nexCognettia H3 nexus file for MrBayes analysisH3_Congettia.nexCognettia ITS nexus file for MrBayes analysisITS_CognettiaMAFFT.nexCognettia 16S run1 p file from MrBayes analysis16S_CognettiaMAFFT.nex.run1.pCognettia 16S run2 p file from MrBayes analysis16S_CognettiaMAFFT.nex.run2.pCognettia COI run1 p file from MrBayes analysisCOI_cognettia.nex.run1.pCognettia COI run2 p file from MrBayes analysisCOI_cognettia.nex.run2.pCognettia H3 run1 p file from MrBayes analysisH3_Congettia.nex.run1.pCognettia H3 run2 p file from MrBayes analysisH3_Congettia.nex.run2.pCognettia ITS run1 p file from MrBayes analysisITS_CognettiaMAFFT.nex.run1.pCognettia ITS run2 p file from MrBayes analysisITS_CognettiaMAFFT.nex.run2.pCognettia 16S parts file from MrBayes analysis16S_CognettiaMAFFT.nex.partsCognettia COI parts file from MrBayes analysisCOI_cognettia.nex.partsCognettia H3 parts file from MrBayes analysisH3_Congettia.nex.partsCognettia ITS parts file from MrBayes analysisITS_CognettiaMAFFT.nex.partsCognettia 16S run1 t file from MrBayes analysis16S_CognettiaMAFFT.nex.run1.tCognettia 16S run2 t file from MrBayes analysis16S_CognettiaMAFFT.nex.run2.tCognettia COI run1 t file from MrBayes analysisCOI_cognettia.nex.run1.tCognettia COI run2 t file from MrBayes analysisCOI_cognettia.nex.run2.tCognettia H3 run1 t file from MrBayes analysisH3_Congettia.nex.run1.tCognettia H3 run2 t file from MrBayes analysisH3_Congettia.nex.run2.tCognettia ITS run1 t file from MrBayes analysisITS_CognettiaMAFFT.nex.run1.tCognettia ITS run2 t file from MrBayes analysisITS_CognettiaMAFFT.nex.run2.tCognettia 16S trprobs file from MrBayes analysis16S_CognettiaMAFFT.nex.trprobsCognettia COI trprobs file from MrBayes analysisCOI_cognettia.nex.trprobsCognettia H3 trprobs file from MrBayes analysisH3_Congettia.nex.trprobsCognettia ITS trprobs file from MrBayes analysisITS_CognettiaMAFFT.nex.trprobs