DOI: 10.17635/lancaster/researchdata/683
title: Time-varying MASCO and multiscale activity alterations following methamphetamine exposure in Per1/2/3 knockout mice - Data

description: All mouse experiments were approved by the Institutional Animal Care and
 Use Committee at UT Southwestern Medical Center (Protocol #2013-0035 and
 2016-10376-G). In the initial investigation, 8 Per1/2/3 KO mice (Yamazaki
 Lab Experiment #74; 4 males, 4 females; 5-8 months old; C57BL/6J or
 C57BL/6J and C57BL/6N mixed backgrounds) were used in a variety of
 conditions. Mice were first exposed to 12~hour light, 12~hour dark cycles
 for 7~days (LD, light intensity 450 lux at cage level) and then kept in
 constant darkness for 11 days (DD). subsequently, methamphetamine was
 administrated through drinking water for 26 days while they remained in
 constant darkness (MDD). The mice were then exposed to constant light for
 22 days while methamphetamine administration continued (MLL, light
 intensity 170 lux at cage level). Following this, methamphetamine was
 removed while still in constant light for 54 days (LL, light intensity 170
 lux at cage level). The final 24 days of the experiment were conducted in
 constant darkness for 24~days (FDD). To further understand the multiscale
 behavioral changes following methamphetamine exposure across elongated
 measurement intervals, three cohorts of mice were subsequently
 investigated. A cohort of heterozygous PER2::LUCIFERASE knockin mice were
 used as a wild-type comparison point (Yamazaki Lab Experiment #17; 5
 males, 3 females; 1.3-9.5 months old; C57BL/6J). These mice were kept in
 constant darkness for 95 days without methamphetamine. General cage
 activity was recorded with an infrared motion detector in the cage without
 a running wheel for the first 10 days, then in a cage containing a locked
 running wheel for 11 days. after that, the running wheel was unlocked and
 both general activity and wheel running activity were recorded for 20
 days. The wheel was then locked once again for a further 21 days, before
 being unlocked for the final 33 days. Here, we analysed the last 30 days
 of running wheel activity. Throughout the manuscript, this cohort is
 referred to as wild-type. A cohort of Per1/2/3 KO mice (Yamazaki Lab
 Experiment #86; 3 males, 4 females; 3.5-8.5 months old; C57BL/6J
 background with cfos-shGFP transgene) were initially exposed to constant
 light (220 lux at cage level) for 26 days then kept in constant darkness
 for 65 days without methamphetamine exposure. One male mouse (#2 in the
 supporting information) was excluded as the full recording length was not
 completed. The 65 days of running wheel activity in constant darkness were
 analysed in this manuscript This group is referred to as Per1/2/3 KO DD
 throughout. Another cohort of Per1/2/3 KO mice (Yamazaki Lab Experiment
 #54; 1 male, 4 females; 4.5-5.5 months old; C57BL/6J or C57BL/6J and
 C57BL/6N mixed background) were kept in DD for 27 days without
 methamphetamine, before being exposed to methamphetamine for the
 subsequent 101 days. The first 65 days of activity during methamphetamine
 administration was analysed and is referred to as Per1/2/3 KO DD
 throughout the manuscript. Each mouse was housed individually in a plastic
 cage (length~×~width~x~height: 29.5×11.5×12.0~cm) containing running
 wheels of diameter 11 cm. Wheel revolutions were continuously recorded
 every minute by the ClockLab system (Actimetrics, Wilmette, IL USA). As
 described above, general cage activity was monitored with a passive
 infrared sensor (product ID 189, Adafruit, New York City, NY, USA) placed
 above the cages without a running wheel or with locked running wheels,
 however those data were not analysed in current study. The cages were
 placed in light-tight ventilated cabinets, and temperature, humidity, and
 light intensity inside of the cabinet were recorded every 5 min by Chamber
 Controller software (Actimetrics, Wilmette, IL USA). The white LEDs inside
 the cabinet were controlled by the Chamber Controller software. Cages and
 water bottles were changed once every 3 weeks. An infrared viewer
 (FIND-R-SCOPE Infrared Viewer; FJW Optical Systems, Inc. Palatine, IL USA)
 was used to perform maintenance in the dark without exposing mice to
 visible light. For methamphetamine administration, water bottles were
 replaced with drinking (tap) water containing 0.005% methamphetamine
 (Sigma-Aldrich, St. Louis, MO USA). Water bottles containing
 methamphetamine were changed once every 3 weeks. During the experiment
 mice had ad liberum access to food and regular or methamphetamine water.
 Data is available upon appropriate request. A segment of this dataset is
 used in a paper currently undergoing peer review. Following the completion
 of this process, the data will be made publicly available. Description