cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7451942
title: Molecular evolution and transcriptional profile of GH3 and GH20 β-N-acetylglucosaminidases in the entomopathogenic fungus Metarhizium anisopliae

description: Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with Aspergilllus sp. and entomopathogenic fungal species. By contrast, MaNAG3 and MaNAG4 were phylogenetically related with bacterial GH3 NAGases. The transcriptional profiles of M. anisopliae NAGase genes were evaluated in seven culture conditions showing no common regulatory patterns, suggesting that these enzymes may have specific roles during the Metarhizium life cycle. Moreover, the expression of MaNAG3 and MaNAG4 regulated by chitinous substrates is the first evidence of the involvement of putative GH3 NAGases in physiological cell processes in entomopathogens, indicating their potential influence on cell differentiation during the M. anisopliae life cycle.
DOI: 10.6084/m9.figshare.7451948.v1
title: Influence of socio-demographic characteristics in the self-care of people with heart failure

description: ABSTRACT Objective: To determine the influence of socio-demographic characteristics in the self-care of people with heart failure (HF). Method: Cross-sectional, analytical study, held in three private hospitals in Fortaleza, Ceará, Brasil, with 57 hospitalized patients. The data were collected through a demographic characterization form and a self-care assessment scale and were analyzed with inferential statistics, using mean comparison tests. Results: Self-care was best assessed in people with higher education level, higher household income and in a relationship. Conclusion: The socio-demographic characteristics influenced seven self-care practices: dietary control; monitoring of body weight; effort in labor activities; knowledge about HF; up-to-date vaccination record; leisure activities; and family and social support network with strong bonds. The higher prevalence of answers indicating satisfactory self-care practices among the patients occurred in the areas of health promotion and tolerance to stress.
DOI: 10.7284/102233
title: Winch data collected during research cruise MV1012 using a Markey DUSH-5 instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7451942.v1
title: Molecular evolution and transcriptional profile of GH3 and GH20 β-N-acetylglucosaminidases in the entomopathogenic fungus Metarhizium anisopliae

description: Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with Aspergilllus sp. and entomopathogenic fungal species. By contrast, MaNAG3 and MaNAG4 were phylogenetically related with bacterial GH3 NAGases. The transcriptional profiles of M. anisopliae NAGase genes were evaluated in seven culture conditions showing no common regulatory patterns, suggesting that these enzymes may have specific roles during the Metarhizium life cycle. Moreover, the expression of MaNAG3 and MaNAG4 regulated by chitinous substrates is the first evidence of the involvement of putative GH3 NAGases in physiological cell processes in entomopathogens, indicating their potential influence on cell differentiation during the M. anisopliae life cycle.
DOI: 10.7284/101866
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise MV1013 using a Hawaii UHDAS instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7451927
title: Evaluation of surfactant activity and emulsifying of Pea protein isolate (Pisum sativum L.) obtained by the spray dryer

description: ABSTRACT This study evaluates the biosurfactant activity and emulsification ability of pea proteins isolated (Pisum sativum L.). The results showed that aqueous solutions containing the pea proteins isolated (PPI) reduce the water surface tension to 47mN/m, approximately. The use of spray dryer does not change the surfactant activity of the PPI because surface tension and CMC will not change. The PPI is thermally stable and can be used in processes that require temperatures between 4°C and 80°C, as industrial processes such as baking, ice cream and other formulation. The protein isolate and pea assessed emulsions with toluene and n-heptane were stable. Emulsions castor and linseed oil were not stable under the conditions reported in this paper. Therefore, despite the pea protein isolate is a surfactant it cannot be used in any industrial process. Both the food industry and in the biotechnology industry should evaluate the conditions for application of protein isolates from pea.
DOI: 10.6084/m9.figshare.7451930
title: Characterization of hibiscus sabdariffa fiber as potential reinforcement for denture acrylic resins

description: ABSTRACT This study aimed to determine the characteristics of Hibiscus sabdariffa as a fiber-reinforcement material for denture acrylic resins. The Hibiscus sabdariffa fiber was extracted by hot water retting. The fiber was treated at room temperature in aqueous solution of NaOH for 8 hours at 8% concentration. Micrometre gauge and Field emission scanning electron microscope (FE-SEM), Archimedes principle, Fourier transform infrared (FTIR) spectroscopy, and Thermogravimetric analyser were used to characterize the diameter, morphology, density, water content and absorption, functional chemical component and thermal behaviour of Hibiscus sabdariffa fiber. In addition, the reinforcing characteristics of Hibiscus sabdariffa fiber was evaluated using denture acrylic resin. The flexural strength was measured in line with ASTM D 790 using LloydTM three point bending machine. In line with ASTM D 256, the impact strength was measured using HounsfieldTM charpy tester. The Micrometre gauge and FE-SEM analysis confirmed that diameter of Hibiscus sabdariffa fiber is within the range of other established reinforcing lignocellulosic fibers so also are the density, moisture content and absorption. The FTIR spectroscopy and TGA analysis confirmed the presence of cellulose, hemicellulose and other components in the fiber which were modified by treatment resulting in decreased hydrophilicity of the fibers thereby improving the thermal stability of the fiber. Furthermore, the bond fiber/matrix adhesion was improved which resulted in improve mechanical properties of the composite. The salient features of this study indicated that based on the suitable properties, Hibiscus sabdariffa fiber can be applied to reinforcing denture acrylics. In addition, and potentially, the use of Hibiscus sabdariffa fiber in reinforcement of denture acrylic resins can trigger further economic benefit while ensuring sustainable, green and safer environment.
DOI: 10.6084/m9.figshare.7451933
title: Evaluation of the employment of eucalyptus bark in the bioadsorption of contaminant petroleum light hydrocarbons in simulated water bodies

description: ABSTRACT The contamination of water bodies by petroleum and its derivatives has been one of the main environmental problems nowadays and it causes many losses, such as ecological imbalance by damage to fauna and flora, and the reduction of the sunlight availability in the aquatic environment. Given this context, this paper proposed the employing of eucalyptus bark for the removal of petroleum light hydrocarbons (commercial gasoline type C) contaminant in simulated freshwater and saltwater environments. Therefore, it was verified the bark ability in adsorbing organic compounds when it was submitted to the contact with tartrazine, rhodamine B and methylene blue dyes, and the latter two had confirmed the chemical affinity of the biomaterial with carbon compounds. Then, in the adsorption of the simulated contaminant solution, it was evaluated the influences of: the biomass particle size, observing a trend toward better adsorption with the smallest particle size of the material, the water body temperature, that did not appreciably affect the adsorption capacity, and the reaction time, which benefited the adsorption in freshwater environment and reduced when the biomass was in contact with the saltwater. Furthermore, in a comparison among eucalyptus bark and other commercial adsorbents (activated charcoal, vermiculite and diatomaceous earth), it had higher adsorption capacity than all of them, proving to be a promising material for the decontamination of water containing petroleum hydrocarbons
DOI: 10.6084/m9.figshare.7451927.v1
title: Evaluation of surfactant activity and emulsifying of Pea protein isolate (Pisum sativum L.) obtained by the spray dryer

description: ABSTRACT This study evaluates the biosurfactant activity and emulsification ability of pea proteins isolated (Pisum sativum L.). The results showed that aqueous solutions containing the pea proteins isolated (PPI) reduce the water surface tension to 47mN/m, approximately. The use of spray dryer does not change the surfactant activity of the PPI because surface tension and CMC will not change. The PPI is thermally stable and can be used in processes that require temperatures between 4°C and 80°C, as industrial processes such as baking, ice cream and other formulation. The protein isolate and pea assessed emulsions with toluene and n-heptane were stable. Emulsions castor and linseed oil were not stable under the conditions reported in this paper. Therefore, despite the pea protein isolate is a surfactant it cannot be used in any industrial process. Both the food industry and in the biotechnology industry should evaluate the conditions for application of protein isolates from pea.
DOI: 10.6084/m9.figshare.7451930.v1
title: Characterization of hibiscus sabdariffa fiber as potential reinforcement for denture acrylic resins

description: ABSTRACT This study aimed to determine the characteristics of Hibiscus sabdariffa as a fiber-reinforcement material for denture acrylic resins. The Hibiscus sabdariffa fiber was extracted by hot water retting. The fiber was treated at room temperature in aqueous solution of NaOH for 8 hours at 8% concentration. Micrometre gauge and Field emission scanning electron microscope (FE-SEM), Archimedes principle, Fourier transform infrared (FTIR) spectroscopy, and Thermogravimetric analyser were used to characterize the diameter, morphology, density, water content and absorption, functional chemical component and thermal behaviour of Hibiscus sabdariffa fiber. In addition, the reinforcing characteristics of Hibiscus sabdariffa fiber was evaluated using denture acrylic resin. The flexural strength was measured in line with ASTM D 790 using LloydTM three point bending machine. In line with ASTM D 256, the impact strength was measured using HounsfieldTM charpy tester. The Micrometre gauge and FE-SEM analysis confirmed that diameter of Hibiscus sabdariffa fiber is within the range of other established reinforcing lignocellulosic fibers so also are the density, moisture content and absorption. The FTIR spectroscopy and TGA analysis confirmed the presence of cellulose, hemicellulose and other components in the fiber which were modified by treatment resulting in decreased hydrophilicity of the fibers thereby improving the thermal stability of the fiber. Furthermore, the bond fiber/matrix adhesion was improved which resulted in improve mechanical properties of the composite. The salient features of this study indicated that based on the suitable properties, Hibiscus sabdariffa fiber can be applied to reinforcing denture acrylics. In addition, and potentially, the use of Hibiscus sabdariffa fiber in reinforcement of denture acrylic resins can trigger further economic benefit while ensuring sustainable, green and safer environment.
DOI: 10.6084/m9.figshare.7451921
title: Study of acrylic resins modified by different particles sizes for application in odontology area.

description: ABSTRACT With the increase in population life expectancy, the search for products with greater durability increases. This leads to search for more and more resistant materials, including in the dental field. Once inorganic particles play a fundamental role in the properties of a composite resin, we searched to evaluate the influence different particles size in its mechanical properties. In these dental resins, we evaluated flexural strength, compressive strength, microhardness Vickers and contraction stress. To avoid unwanted variations, the consistency of these resins did not differ between samples (Consistency Squeeze = 75 N). Besides consistency, other variables of a composite resin (organic matrix composition, initiators, inhibitors) had their amounts fixed, seeking no other influence on the study. Nine types of resins were used in this study, with an average particle size ranging between 0.4μm and 3.0μm, there are three resins composed of single particles (0.4µm, 1.0µm e 3.0 µm) and six resins composed by mixing particles mentioned above. The results were compiled and the data submitted to statistical analysis (ANOVA – Multiple Comparisons Tukey test with 5% significance). The results showed that the synergistic effect of the mixture of particles reflected better properties in compressive strength and flexural strength test as compared to resins with single-size particles. Is possible to conclude too that there is not a single average size range of particles with results superior to other resins in all properties. However, looking for a balance between these properties, samples with an average particle size of 2.02μm and 2.14μm both composed of mixtures of particles (no single particles) showed the best results. As showed by Tukey's statistical analysis, both resins were placed as belonging to the 2 groups that indicate superior properties in all tests.
DOI: 10.6084/m9.figshare.7451924
title: Reuse of residual diatomaceous earth from manufacturing of antiophidic and antitoxic sera

description: ABSTRACT In the production of antiophidic and antitoxic sera, it is common to use diatomaceous earth, which is a light mineral, with low specific mass and high adsorption potential, commonly employed in filtration processes. In order to reuse the residual earth from filtration processes of antiophidic and antitoxic sera of Fundação Ezequiel Dias, this work purposed its recuperation by thermal treatment. After Thermogravimetric Analysis of the residual diatomaceous earth, it was concluded that from 600°C all the impurities present in this material suffered degradation Thus, a sample of saturated mineral was heated in a muffle oven up to temperature of 700°C. Then, the influence of the thermal treatment in the mineral properties was evaluated by means of the determination of the physicochemical parameters pH, apparent density, dry basis moisture content and its adsorption potential in hiperimune horse plasma, the main characteristic for its application in the filtration process to obtain the sera. It was verified that the physicochemical properties of the recuperated material were very close to them of the new earth, being obtained percentage variations of 6.1% for pH, 12.8% for apparent density, 3.6% for basis moisture content and 1.4% in relation to relative adsorption percentage. Therefore, it was possible to infer that that the purposed thermal treatment did not vary its adsorption capability considerably, making possible to reuse it as a filtration agent in the sera production process.
DOI: 10.6084/m9.figshare.7451933.v1
title: Evaluation of the employment of eucalyptus bark in the bioadsorption of contaminant petroleum light hydrocarbons in simulated water bodies

description: ABSTRACT The contamination of water bodies by petroleum and its derivatives has been one of the main environmental problems nowadays and it causes many losses, such as ecological imbalance by damage to fauna and flora, and the reduction of the sunlight availability in the aquatic environment. Given this context, this paper proposed the employing of eucalyptus bark for the removal of petroleum light hydrocarbons (commercial gasoline type C) contaminant in simulated freshwater and saltwater environments. Therefore, it was verified the bark ability in adsorbing organic compounds when it was submitted to the contact with tartrazine, rhodamine B and methylene blue dyes, and the latter two had confirmed the chemical affinity of the biomaterial with carbon compounds. Then, in the adsorption of the simulated contaminant solution, it was evaluated the influences of: the biomass particle size, observing a trend toward better adsorption with the smallest particle size of the material, the water body temperature, that did not appreciably affect the adsorption capacity, and the reaction time, which benefited the adsorption in freshwater environment and reduced when the biomass was in contact with the saltwater. Furthermore, in a comparison among eucalyptus bark and other commercial adsorbents (activated charcoal, vermiculite and diatomaceous earth), it had higher adsorption capacity than all of them, proving to be a promising material for the decontamination of water containing petroleum hydrocarbons
DOI: 10.7284/101865
title: Expendable Probe data collected during research cruise MV1013 using a Sippican MK21 instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7451921.v1
title: Study of acrylic resins modified by different particles sizes for application in odontology area.

description: ABSTRACT With the increase in population life expectancy, the search for products with greater durability increases. This leads to search for more and more resistant materials, including in the dental field. Once inorganic particles play a fundamental role in the properties of a composite resin, we searched to evaluate the influence different particles size in its mechanical properties. In these dental resins, we evaluated flexural strength, compressive strength, microhardness Vickers and contraction stress. To avoid unwanted variations, the consistency of these resins did not differ between samples (Consistency Squeeze = 75 N). Besides consistency, other variables of a composite resin (organic matrix composition, initiators, inhibitors) had their amounts fixed, seeking no other influence on the study. Nine types of resins were used in this study, with an average particle size ranging between 0.4μm and 3.0μm, there are three resins composed of single particles (0.4µm, 1.0µm e 3.0 µm) and six resins composed by mixing particles mentioned above. The results were compiled and the data submitted to statistical analysis (ANOVA – Multiple Comparisons Tukey test with 5% significance). The results showed that the synergistic effect of the mixture of particles reflected better properties in compressive strength and flexural strength test as compared to resins with single-size particles. Is possible to conclude too that there is not a single average size range of particles with results superior to other resins in all properties. However, looking for a balance between these properties, samples with an average particle size of 2.02μm and 2.14μm both composed of mixtures of particles (no single particles) showed the best results. As showed by Tukey's statistical analysis, both resins were placed as belonging to the 2 groups that indicate superior properties in all tests.
DOI: 10.6084/m9.figshare.7451924.v1
title: Reuse of residual diatomaceous earth from manufacturing of antiophidic and antitoxic sera

description: ABSTRACT In the production of antiophidic and antitoxic sera, it is common to use diatomaceous earth, which is a light mineral, with low specific mass and high adsorption potential, commonly employed in filtration processes. In order to reuse the residual earth from filtration processes of antiophidic and antitoxic sera of Fundação Ezequiel Dias, this work purposed its recuperation by thermal treatment. After Thermogravimetric Analysis of the residual diatomaceous earth, it was concluded that from 600°C all the impurities present in this material suffered degradation Thus, a sample of saturated mineral was heated in a muffle oven up to temperature of 700°C. Then, the influence of the thermal treatment in the mineral properties was evaluated by means of the determination of the physicochemical parameters pH, apparent density, dry basis moisture content and its adsorption potential in hiperimune horse plasma, the main characteristic for its application in the filtration process to obtain the sera. It was verified that the physicochemical properties of the recuperated material were very close to them of the new earth, being obtained percentage variations of 6.1% for pH, 12.8% for apparent density, 3.6% for basis moisture content and 1.4% in relation to relative adsorption percentage. Therefore, it was possible to infer that that the purposed thermal treatment did not vary its adsorption capability considerably, making possible to reuse it as a filtration agent in the sera production process.
DOI: 10.6084/m9.figshare.7451912
title: Preparation of PHB membranes by electrospinning and characterization for tissue engineering applications

description: ABSTRACT This work aims the preparing of mats composed of poly (3-hydroxybutyrate) -(PHB) biodegradable polymeric fibers, using the electrospinning technique. Mats were obtained under different conditions: changing the flow and temperature of the solution, tension and the addition of a surfactant to the solution. The obtained material was analyzed by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermogravimetry (TG), X ray diffractometry (XRD) and cell viability. The characterization by SEM showed that the best results, presenting fibers with constant diameters of 0.77 μm and without beads formation, were reached under the following conditions: concentration of 10% (m / v) solution in CF / DMF (9/1), solution flow of 5.8 ml/h, 17 kV and addition of surfactant (sodium dodecylsulfate, SDS). The results showed that the variation of the flow, tension and surfactant did not influence the average diameter of the fibers, but the increase of the temperature decreases this parameter. Moreover, the addition of a surfactant increases the density of the blankets and interconnect the pores, allowing cellular proliferation, and, thus. it is indicated for use as scaffolds.