cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.d45trx
title: Occurrence Download

description: A dataset containing 280 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ailuropoda melanoleuca"
 ]
}

 The dataset includes 280 records from 35 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047835-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/k32d2-ba288
title: MamJ deletion w/o iron

description: No magnetosome vesicles, suspected as a mutant that has lost the magnetosome island
description: <strong>Tilt Series Date:</strong> 2010-01-22</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum</p>
<strong>Strain:</strong> AMB-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> Raptor and Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> Mutant constructed by Arash Komeili, cells shipped to and imaged by Zhuo Li</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Stationary phase</p>
<strong>Sample Preparation:</strong> Quantifoid grid, 10-nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-01-22-3/rawdata/</p>10jan22a_dJnofe2_006.mrc,  Tilt Series (Pixel Size 0.0 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-01-22-3/rawdata/10jan22a_dJnofe2_006.mrc"
        > <i class="download icon"></i> Download </a></p>

        10jan22a_dJnofe2_006_part.rec,  Reconstruction (Pixel Size 0.0 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-01-22-3/3dimage_9174/10jan22a_dJnofe2_006_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rrf8pq
title: Occurrence Download

description: A dataset containing 2233 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-92.33200 -52.65838,53.33200 -52.65838,53.33200 5.65838,-92.33200 5.65838,-92.33200 -52.65838))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Buteogallus coronatus"
 ]
}

 The dataset includes 2233 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047836-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/03v9x-ptq88
title: Pseudoalteromonas Iuteoviolacea MP129

description: P Iuteo DvipABhcpI Dbact2 whole cells from liquid culture
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab, taken at Janelia Farms</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid culture</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-7/rawdata/</p>2013_07_12_JF_MP129_0004.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-7/rawdata/2013_07_12_JF_MP129_0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_07_12_JF_MP129_0004_2k.rec,  Reconstruction (Pixel Size 1.68 nm), 409.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-7/3dimage_28928/2013_07_12_JF_MP129_0004_2k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/as3aa-nwa68
title: B. subtilis 3267

description: Raw data files of B. subtilis 3267
description: <strong>Tilt Series Date:</strong> 2011-12-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> AD3267, ponA null, Alp7A-GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joe Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow in LB</p>
<strong>Sample Preparation:</strong> Polara</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-12-11-2/rawdata/</p>3267c0002.mrc,  Tilt Series (Pixel Size 1.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-12-11-2/rawdata/3267c0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        3267c0002_part.rec,  Reconstruction (Pixel Size 3.8 nm), 929.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-12-11-2/3dimage_16166/3267c0002_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r7az3-4hq34
title: Hyphomonas session11 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-03-29</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-15/rawdata/</p>09mar29a_bx608a_015.mrc,  Tilt Series (Pixel Size 0.632 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-15/rawdata/09mar29a_bx608a_015.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar29a_bx608a_015_full.rec,  Reconstruction (Pixel Size 1.264 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-15/3dimage_7307/09mar29a_bx608a_015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nynn3-anw25
title: Agrobacterium delta rem

description: Agrobacterium tumefaciens non-motile delta rem mutant
description: <strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-14/rawdata/</p>delta_rem0001.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-14/rawdata/delta_rem0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        delta_rem0001.rec,  Reconstruction (Pixel Size 2.24 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-14/3dimage_40979/delta_rem0001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/34tej-2nr11
title: Hyphomonas session16 27.5KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-06-06</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in 2216 media, 4degree...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-06-14/rawdata/</p>09jun06a_bx823a_046.mrc,  Tilt Series (Pixel Size 0.774 nm), 520.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-06-14/rawdata/09jun06a_bx823a_046.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun06a_bx823a_046_part.rec,  Reconstruction (Pixel Size 1.548 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-06-14/3dimage_7884/09jun06a_bx823a_046_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bw3j1-m7g73
title: Wild type control

description: Raw data files of Wild type control
description: <strong>Tilt Series Date:</strong> 2009-08-01</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, PEACH</p>
<strong>Collaborators and Roles:</strong> Experiments were done by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Exponential phase NA1000 cells.</p>
<strong>Sample Preparation:</strong> 10 nm gold </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-117/rawdata/</p>09aug01a_noadd1a_117.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-117/rawdata/09aug01a_noadd1a_117.mrc"
        > <i class="download icon"></i> Download </a></p>

        09aug01a_noadd1a_117_full.rec,  Reconstruction (Pixel Size 2.4 nm), 367.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-08-01-117/3dimage_6323/09aug01a_noadd1a_117_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3jswm-jx522
title: HUVECs-pNLGFP-VPS4DN

description: HUVECs cells were first transfected with VPS4DN construct using Nucleofector Kit. 4.5 h post-transfection cells were infected with pNLGFP-VSVG by spinoculation and then frozen 24 h post infection.  Sample# C68
description: <strong>Tilt Series Date:</strong> 2011-04-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNL4GFP provirus construct from Irvin Chen (UCLA). Cells transfected, infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids, transfected and then infected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-04-14-10/rawdata/</p>11apr14b_C68-2b_019.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-04-14-10/rawdata/11apr14b_C68-2b_019.st"
        > <i class="download icon"></i> Download </a></p>

        11apr14b_C68-2b_019_full.rec,  Reconstruction (Pixel Size 2.0 nm), 587.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-04-14-10/3dimage_68290/11apr14b_C68-2b_019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4y2d4-w9875
title: Agrobacterium tumefaciens C58

description: Agro C58
description: <strong>Tilt Series Date:</strong> 2013-10-23</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Induction for T-pilus with 200uM AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-15/rawdata/</p>c58_berk_home_195k0015.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-15/rawdata/c58_berk_home_195k0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_berk_home_195k0015.rec,  Reconstruction (Pixel Size 0.0 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-15/3dimage_49009/c58_berk_home_195k0015.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qd3sg-5bt95
title: BsubtilisMJ007

description: Bsubtilis MJ007, skinny Bacillus
description: <strong>Tilt Series Date:</strong> 2011-08-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-02-30/3dimage_24779/</p>mj007_20006_full.rec,  Reconstruction (Pixel Size 1.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-02-30/3dimage_24779/mj007_20006_full.rec"
        > <i class="download icon"></i> Download </a></p>

        mj007_20006.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-02-30/rawdata/mj007_20006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/snm90-qp045
title: PAO1 Wild Type

description: Pseudomonas aeruginosa PAO1 Wild Type
description: <strong>Tilt Series Date:</strong> 2014-10-14</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-19/rawdata/</p>PAOIWTMCTest0009_mcorr.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-19/rawdata/PAOIWTMCTest0009_mcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        PAOIWTMCTest0009_mcorr_full.rec,  Reconstruction (Pixel Size 1.28 nm), 688.9 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.9cq668
title: Occurrence Download

description: A dataset containing 14972 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Trigona spinipes)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 14972 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047837-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/v5dg5-bq347
title: gold labeled CheA- in vitro

description: post wash sample - gold labeled CheA, 1.8 nm gold, post ternary formation snap rxn.
description: <strong>Tilt Series Date:</strong> 2013-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Heather Hodge and Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Buffer: 1x Snap + 1mM DTT 50 mM Tris Hcl pH 7.5, 100 mM Ncl, 0.1% Tween-200)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-86/rawdata/</p>HTH1B_10040.mrc,  Tilt Series (Pixel Size 0.2989 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-86/rawdata/HTH1B_10040.mrc"
        > <i class="download icon"></i> Download </a></p>

        HTH1B_10040_part67_3.rec,  Reconstruction (Pixel Size 0.5978 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-86/3dimage_26528/HTH1B_10040_part67_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zb8gh-whq02
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-22/3dimage_69403/</p>12may29_C135_1_MEFnomitoDNA_AT2BT20013_full.rec,  Reconstruction (Pixel Size 2.0 nm), 2.9 GB  
        
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        12may29_C135_1_MEFnomitoDNA_AT2BT20013.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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