DOI: 10.5061/dryad.fbg79cp62
title: Data from: Artabotrys rubriflorus (Annonaceae), a new species from Yunnan China

description: Artabotrys rubriflorus sp. nov. is described as a new species of
 Annonaceae, collected from Malipo, Yunnan Province, China.The specimens of
 this new species were gathered through fieldwork and were subsequently
 subjected to rigorous analysis using both classical taxonomical methods
 and molecular phylogenetic analysis. The phylogenetic reconstruction,
 based on four chloroplast DNA regions (matK, rbcL, trnLF, and psbAtrnH),
 was carried out employing maximum parsimony, maximum likelihood and
 Bayesian inference approaches. These analyses confirmed the systematic
 positioning of Artabotrys rubriflorus sp. nov. within the genus
 Artabotrys, while also establishing its distinctiveness from other
 species.
description: Chloroplast genomic DNA was extracted from collected
 silica-dried leaves (Narzary et al. 2015) using the Ezup Spin Column Super
 Plant Genomic DNA Extraction Kit (Sangon Biotech, Shanghai). Four
 chloroplast DNA regions (matK, rbcL, trnL-F and psbA-trnH) were subjected
 to PCR amplification. Primer information is given in Table 3. The PCR
 reaction system (25 µl) consisted of 15 µl of 2 Taq Master Mix buffer, 10
 µl of ddH2O, 3 µl of the primer mixture (1.5 µl each of the positive and
 reverse primer), and 2 µl of the DNA template. The parameters for PCR
 procedure was 95 for 3 min, followed by 40 cycles of 95 for 30 s,
 appropriate annealing temperature for 30 s, 72 for 1 min, and 10 min for
 final extension at 72 , the annealing temperatures was 54 for matK and
 rbcL genes, 49 for trnL-F and psbA-trnH intergenic spacers. 5 µl of the
 products were tested using electrophoresis in 1% agarose gel at 150 V, 110
 mA for 10 to 20 min. San Prep Column DNA Gel Extraction Kit (Sangon
 Biotech, Shanghai) was used for recovery and purification, then the
 purified products were sequenced by Sangon Biotech (Shanghai, China 
 https://www.sangon.com/).
description: # Data from: Artabotrys rubriflorus (Annonaceae), a new species from
 Yunnan China
 [https://doi.org/10.5061/dryad.fbg79cp62](https://doi.org/10.5061/dryad.fbg79cp62) ## Description of the data and file structure Chloroplast genomic DNA was extracted from collected silica-dried leaves (Narzary et al. 2015) using the Ezup Spin Column Super Plant Genomic DNA Extraction Kit (Sangon Biotech, Shanghai). Four chloroplast DNA regions (matK, rbcL, trnL-F and psbA-trnH) were subjected to PCR amplification. The PCR reaction system (25 µl) consisted of 15 µl of 2 Taq Master Mix buffer, 10 µl of ddH2O, 3 µl of the primer mixture (1.5 µl each of the positive and reverse primer), and 2 µl of the DNA template. The parameters for PCR procedure was 95 for 3 min, followed by 40 cycles of 95 for 30 s, appropriate annealing temperature for 30 s, 72 for 1 min, and 10 min for final extension at 72 , the annealing temperatures was 54 for matK and rbcL genes, 49 for trnL-F and psbA-trnH intergenic spacers. 5 µl of the products were tested using electrophoresis in 1% agarose gel at 150 V, 110 mA for 10 to 20 min. San Prep Column DNA Gel Extraction Kit (Sangon Biotech, Shanghai) was used for recovery and purification, then the purified products were sequenced by Sangon Biotech (Shanghai, China – [https://www.sangon.com/](https://www.sangon.com/)). ### Files and variables #### File: Rawdata.fasta **Description:** Total 23 sequences of 5 DNA regeions (matK, rbcL, trnLF and psbAtrnH) from 5 different species of genus *Artabotrys* (*Artabotrys fragrans*, *Artabotrys hainanensis*, *Artabotrys pachypetalus*, *Artabotrys pilosus*, and *Artabotrys rubriflorus*).