cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6152156.v1
title: Frequency and carbapenems susceptibility profile of non-fermenting Gram-negative bacilli isolated from clinical samples between 2007 and 2012

description: ABSTRACT Introduction: One of the major problems in health services is the occurrence of healthcare-associated infections (HAIs) by microorganisms resistant to various antimicrobials. Objectives: To describe the frequency and susceptibility profile of Pseudomonas aeruginosa and Acinetobacter baumannii to carbapenems in the hospital from Fundação Santa Casa de Franca, São Paulo, Brazil. Methods: The susceptibility of P. aeruginosa and A. baumannii to carbapenems from 304 clinical isolates between 2007 and 2012 was retrospectively analyzed from a microbiology database at the clinical laboratory of the hospital of Fundação Santa Casa de Franca, São Paulo, Brazil. Results: From isolated and identified strains, 236 (5.3%) P. aeruginosa were susceptible to imipenem (2007 - 69.6% to 2012 - 41.7%) and meropenem (2007 - 63.3% to 2012 - 25%). In addition, all 68 (1.7%) A. baumannii isolates were susceptible to both antibiotics. Conclusion: A. baumannii resistance to carbapenems was not identified; however, there was a decrease in susceptibility to carbapenems over the years for P. aeruginosa.
DOI: 10.6084/m9.figshare.6152153
title: Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene

description: Abstract The human C-C chemokine receptor type-5 (CCR5) is the major transmembrane co-receptor that mediates HIV-1 entry into target CD4+ cells. Gene therapy to knock-out the CCR5 gene has shown encouraging results in providing a functional cure for HIV-1 infection. In gene therapy strategies, the initial region of the CCR5 gene is a hotspot for producing functional gene knock-out. Such target gene editing can be done using programmable endonucleases such as transcription activator-like effector nucleases (TALEN) or clustered regularly interspaced short palindromic repeats (CRISPR-Cas9). These two gene editing approaches are the most modern and effective tools for precise gene modification. However, little is known of potential differences in the efficiencies of TALEN and CRISPR-Cas9 for editing the beginning of the CCR5 gene. To examine which of these two methods is best for gene therapy, we compared the patterns and amount of editing at the beginning of the CCR5 gene using TALEN and CRISPR-Cas9 followed by DNA sequencing. This comparison revealed that CRISPR-Cas9 mediated the sorting of cells that contained 4.8 times more gene editing than TALEN+ transfected cells.
DOI: 10.6084/m9.figshare.6152150
title: Allogeneic mesenchymal stem cells and xenogenic platelet rich plasma, associated or not, in the repair of bone failures in rabbits with secondary osteoporosis

description: Abstract Purpose: To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism. Methods: Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment. Results: A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others. Conclusion: The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p&lt;0.01), when compared to the other treatments.
DOI: 10.6084/m9.figshare.6152153.v1
title: Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene

description: Abstract The human C-C chemokine receptor type-5 (CCR5) is the major transmembrane co-receptor that mediates HIV-1 entry into target CD4+ cells. Gene therapy to knock-out the CCR5 gene has shown encouraging results in providing a functional cure for HIV-1 infection. In gene therapy strategies, the initial region of the CCR5 gene is a hotspot for producing functional gene knock-out. Such target gene editing can be done using programmable endonucleases such as transcription activator-like effector nucleases (TALEN) or clustered regularly interspaced short palindromic repeats (CRISPR-Cas9). These two gene editing approaches are the most modern and effective tools for precise gene modification. However, little is known of potential differences in the efficiencies of TALEN and CRISPR-Cas9 for editing the beginning of the CCR5 gene. To examine which of these two methods is best for gene therapy, we compared the patterns and amount of editing at the beginning of the CCR5 gene using TALEN and CRISPR-Cas9 followed by DNA sequencing. This comparison revealed that CRISPR-Cas9 mediated the sorting of cells that contained 4.8 times more gene editing than TALEN+ transfected cells.
DOI: 10.6084/m9.figshare.6152150.v1
title: Allogeneic mesenchymal stem cells and xenogenic platelet rich plasma, associated or not, in the repair of bone failures in rabbits with secondary osteoporosis

description: Abstract Purpose: To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism. Methods: Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment. Results: A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others. Conclusion: The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p&lt;0.01), when compared to the other treatments.
DOI: 10.6084/m9.figshare.6152147
title: Effective population size and the genetic consequences of commercial whaling on the humpback whales (Megaptera novaeangliae) from Southwestern Atlantic Ocean

description: Abstract Genotypes of 10 microsatellite loci of 420 humpback whales from the Southwestern Atlantic Ocean population were used to estimate for the first time its contemporary effective (Ne) and census (Nc) population sizes and to test the genetic effect of commercial whaling. The results are in agreement with our previous studies that found high genetic diversity for this breeding population. Using an approximate Bayesian computation approach, the scenario of constant Ne was significantly supported over scenarios with moderate to strong size changes during the commercial whaling period. The previous generation Nc (Ne multiplied by 3.6), which should corresponds to the years between around 1980 and 1990, was estimated between ~2,600 and 6,800 whales (point estimate ~4,000), and is broadly compatible with the recent abundance surveys extrapolated to the past using a growth rate of 7.4% per annum. The long-term Nc in the constant scenario (point estimate ~15,000) was broadly compatible (considering the confidence interval) with pre-whaling catch records estimates (point estimate ~25,000). Overall, our results shown that the Southwestern Atlantic Ocean humpback whale population is genetically very diverse and resisted well to the strong population reduction during commercial whaling.
DOI: 10.6084/m9.figshare.6152147.v1
title: Effective population size and the genetic consequences of commercial whaling on the humpback whales (Megaptera novaeangliae) from Southwestern Atlantic Ocean

description: Abstract Genotypes of 10 microsatellite loci of 420 humpback whales from the Southwestern Atlantic Ocean population were used to estimate for the first time its contemporary effective (Ne) and census (Nc) population sizes and to test the genetic effect of commercial whaling. The results are in agreement with our previous studies that found high genetic diversity for this breeding population. Using an approximate Bayesian computation approach, the scenario of constant Ne was significantly supported over scenarios with moderate to strong size changes during the commercial whaling period. The previous generation Nc (Ne multiplied by 3.6), which should corresponds to the years between around 1980 and 1990, was estimated between ~2,600 and 6,800 whales (point estimate ~4,000), and is broadly compatible with the recent abundance surveys extrapolated to the past using a growth rate of 7.4% per annum. The long-term Nc in the constant scenario (point estimate ~15,000) was broadly compatible (considering the confidence interval) with pre-whaling catch records estimates (point estimate ~25,000). Overall, our results shown that the Southwestern Atlantic Ocean humpback whale population is genetically very diverse and resisted well to the strong population reduction during commercial whaling.
DOI: 10.6084/m9.figshare.6152144
title: Corrosion Behavior of API 5L X80 Steel in the Produced Water of Onshore Oil Recovery Facilities

description: High-strength low-alloy steel is used in pipelines, which transport oil/gas and also produced water in the process of the secondary recovery of oil. This paper investigates the corrosion of X80 steel after immersion in static systems (biotic and abiotic). The corrosion rate, the corrosion morphology and the hardness of the coupons were investigated. The corrosion products were determined by SEM/EDS and XRD. The corrosion rates of the coupons in the biotic system were moderate and had an agressive pit morphology, while those of the coupons in the abiotic system were low with less aggressive pit. The hardness of the coupons after exposure to the systems of produced water was not changed compared with that of the control coupons. The corrosion products formed in the biotic system were Fe(OH)3, Fe(OH)2, FeOOH, Fe3O4, FeS2, FeCl3, and in the abiotic system, NaCl, CaCl2 and SrSO4. BaSO4 and CaCO3 were identified in both systems.
DOI: 10.6084/m9.figshare.6152144.v1
title: Corrosion Behavior of API 5L X80 Steel in the Produced Water of Onshore Oil Recovery Facilities

description: High-strength low-alloy steel is used in pipelines, which transport oil/gas and also produced water in the process of the secondary recovery of oil. This paper investigates the corrosion of X80 steel after immersion in static systems (biotic and abiotic). The corrosion rate, the corrosion morphology and the hardness of the coupons were investigated. The corrosion products were determined by SEM/EDS and XRD. The corrosion rates of the coupons in the biotic system were moderate and had an agressive pit morphology, while those of the coupons in the abiotic system were low with less aggressive pit. The hardness of the coupons after exposure to the systems of produced water was not changed compared with that of the control coupons. The corrosion products formed in the biotic system were Fe(OH)3, Fe(OH)2, FeOOH, Fe3O4, FeS2, FeCl3, and in the abiotic system, NaCl, CaCl2 and SrSO4. BaSO4 and CaCO3 were identified in both systems.
DOI: 10.6084/m9.figshare.6152138
title: Effects of Solidification Rate in the Microstructure of Al-Si5Cu3 Aluminum Cast Alloy

description: Aluminium alloy Al-Si5Cu3 (319) is one of the most commonly used casting alloys for automobile components, due to its casting characteristics. Iron is the major impurity element that influences the detrimental β-phase formation in secondary aluminum alloys. In the present study, we investigated the influence of the cooling rate on the formation of iron compounds, α-Al15(Mn,Fe)3Si2 and β-Fe5AlSi phases, in Al-Si5Cu3 containing 1.0% Fe, 0.5% Mn and 0.2% Mg, by thermal analysis and metallography. The results show that the high cooling rates, between 10-103 K/s, are able to reduce and nullify the formation of needle-like β-Al5FeSi phase, and help the formation of the Chinese scripts α-Al15(Fe,Mn)3Si2 phase. The analysis of the curves reveals that the increase of the cooling rate increases the temperature of nucleation of the α-Al dendrite and decrease the eutectics Al-Si and Al-Cu phases.
DOI: 10.6084/m9.figshare.6152138.v1
title: Effects of Solidification Rate in the Microstructure of Al-Si5Cu3 Aluminum Cast Alloy

description: Aluminium alloy Al-Si5Cu3 (319) is one of the most commonly used casting alloys for automobile components, due to its casting characteristics. Iron is the major impurity element that influences the detrimental β-phase formation in secondary aluminum alloys. In the present study, we investigated the influence of the cooling rate on the formation of iron compounds, α-Al15(Mn,Fe)3Si2 and β-Fe5AlSi phases, in Al-Si5Cu3 containing 1.0% Fe, 0.5% Mn and 0.2% Mg, by thermal analysis and metallography. The results show that the high cooling rates, between 10-103 K/s, are able to reduce and nullify the formation of needle-like β-Al5FeSi phase, and help the formation of the Chinese scripts α-Al15(Fe,Mn)3Si2 phase. The analysis of the curves reveals that the increase of the cooling rate increases the temperature of nucleation of the α-Al dendrite and decrease the eutectics Al-Si and Al-Cu phases.
DOI: 10.6084/m9.figshare.6152135
title: Evaluation of Influence of Salt in the Cement Hydration to Oil Wells

description: The influence of NaCl salt in the hydration of the Portland cement paste class G was studied using a Non Conventional Differential Thermal Analysis (NCDTA) on the first 24 hours of cement hydration. The mass of salt used corresponds to 0, 5, 10, 15 and 20% of mass of water used in the preparation of the pastes. The water/cement ratio (w/c) remained constant, being 0.46, and the pastes final volume was 99.9mL. Applying a deconvolution method to separate overlapped NCDTA peaks it was possible to estimate the energy released during the different stages of hydration and the effect of salt in cement hydration process. The presence of up to 10% of salt accelerated the hydration process and increased the hydration of C3S and C3A, while the 20% of the salt causes a delayed hydration and decreased the hydration of C3S and C3A. Futhermore, cement pastes with the same percentages of NaCl and with different hydration times were evaluated by Thermogravimetry (TG), Derivative Thermogravimetry (DTG) and Differential Thermal Analysis (DTA). After 28 days the presence of salt caused a greater increase in the formation of hydrated products.
DOI: 10.6084/m9.figshare.6152135.v1
title: Evaluation of Influence of Salt in the Cement Hydration to Oil Wells

description: The influence of NaCl salt in the hydration of the Portland cement paste class G was studied using a Non Conventional Differential Thermal Analysis (NCDTA) on the first 24 hours of cement hydration. The mass of salt used corresponds to 0, 5, 10, 15 and 20% of mass of water used in the preparation of the pastes. The water/cement ratio (w/c) remained constant, being 0.46, and the pastes final volume was 99.9mL. Applying a deconvolution method to separate overlapped NCDTA peaks it was possible to estimate the energy released during the different stages of hydration and the effect of salt in cement hydration process. The presence of up to 10% of salt accelerated the hydration process and increased the hydration of C3S and C3A, while the 20% of the salt causes a delayed hydration and decreased the hydration of C3S and C3A. Futhermore, cement pastes with the same percentages of NaCl and with different hydration times were evaluated by Thermogravimetry (TG), Derivative Thermogravimetry (DTG) and Differential Thermal Analysis (DTA). After 28 days the presence of salt caused a greater increase in the formation of hydrated products.
DOI: 10.6084/m9.figshare.6152132
title: Resistance to Pitting Corrosion in Steels Based on the Fe-Cr-Ni-C System

description: This study aimed to investigate the effects of difference in nickel content and the tempering temperatures on the corrosion resistance in 13Cr2Ni0.1C and 13Cr1Ni0.15C steels. Results showed that passive film in 13Cr2Ni0.1C steel is formed more quickly at the lowest and highest tempering temperature (650°C and 750°C) but the lowest tempering temperature (650°C) showed better resistance to corrosion pitting. There was passive film formation and pitting corrosion in all tempering temperatures of the 13Cr1Ni0.15C steel and changes in tempering temperature does not significantly alter polarization curves, showing similar behavior to steel 13Cr2Ni0.1C tempered at 650°C.
DOI: 10.6084/m9.figshare.6152132.v1
title: Resistance to Pitting Corrosion in Steels Based on the Fe-Cr-Ni-C System

description: This study aimed to investigate the effects of difference in nickel content and the tempering temperatures on the corrosion resistance in 13Cr2Ni0.1C and 13Cr1Ni0.15C steels. Results showed that passive film in 13Cr2Ni0.1C steel is formed more quickly at the lowest and highest tempering temperature (650°C and 750°C) but the lowest tempering temperature (650°C) showed better resistance to corrosion pitting. There was passive film formation and pitting corrosion in all tempering temperatures of the 13Cr1Ni0.15C steel and changes in tempering temperature does not significantly alter polarization curves, showing similar behavior to steel 13Cr2Ni0.1C tempered at 650°C.
DOI: 10.6084/m9.figshare.6152129
title: The potential contribution of the natural products from Brazilian biodiversity to bioeconomy

description: ABSTRACT The development of our society has been based on the use of biodiversity, especially for medicines and nutrition. Brazil is the nation with the largest biodiversity in the world accounting for more than 15% of all living species. The devastation of biodiversity in Brazil is critical and may not only cause the loss of species and genes that encode enzymes involved in the complex metabolism of organisms, but also the loss of a rich chemical diversity, which is a potential source for bioeconomy based on natural products and new synthetic derivatives. Bioeconomy focus on the use of bio-based products, instead of fossil-based ones and could address some of the important challenges faced by society. Considering the chemical and biological diversity of Brazil, this review highlights the Brazilian natural products that were successfully used to develop new products and the value of secondary metabolites from Brazilian biodiversity with potential application for new products and technologies. Additionally, we would like to address the importance of new technologies and scientific programs to support preservation policies, bioeconomy and strategies for the sustainable use of biodiversity.
DOI: 10.6084/m9.figshare.6152129.v1
title: The potential contribution of the natural products from Brazilian biodiversity to bioeconomy

description: ABSTRACT The development of our society has been based on the use of biodiversity, especially for medicines and nutrition. Brazil is the nation with the largest biodiversity in the world accounting for more than 15% of all living species. The devastation of biodiversity in Brazil is critical and may not only cause the loss of species and genes that encode enzymes involved in the complex metabolism of organisms, but also the loss of a rich chemical diversity, which is a potential source for bioeconomy based on natural products and new synthetic derivatives. Bioeconomy focus on the use of bio-based products, instead of fossil-based ones and could address some of the important challenges faced by society. Considering the chemical and biological diversity of Brazil, this review highlights the Brazilian natural products that were successfully used to develop new products and the value of secondary metabolites from Brazilian biodiversity with potential application for new products and technologies. Additionally, we would like to address the importance of new technologies and scientific programs to support preservation policies, bioeconomy and strategies for the sustainable use of biodiversity.
DOI: 10.6084/m9.figshare.6152123
title: Embrittlement and Corrosion Decay of a Cast Duplex Stainless Steel

description: Duplex stainless steels (DSS) are Fe-Cr-Ni-Mo alloys, with low carbon content, which have a favorable combination of mechanical properties and corrosion resistance. These characteristics are mainly attributed to a dual phase and refined microstructure, typical of these alloys, consisting of similar amounts of ferrite and austenite. For this reason, DSS are widely used in various segments of industry, especially in offshore applications. However, DSS components may have a decrease of their properties when they are exposed high temperatures, often due to incorrect manufacturing processes. Intermetallic phases may precipitate causing embrittlement and corrosion resistance decrease. This work presents the effects of short time aging at 475 °C on the mechanical properties and localized corrosion resistance of a cast DSS. The results show that mechanical properties, such as toughness and hardness, are more susceptible to aging, having already been affected during the first 4 hours of heat treatment, while the corrosion was affected in 12 h of aging.
DOI: 10.6084/m9.figshare.6152123.v1
title: Embrittlement and Corrosion Decay of a Cast Duplex Stainless Steel

description: Duplex stainless steels (DSS) are Fe-Cr-Ni-Mo alloys, with low carbon content, which have a favorable combination of mechanical properties and corrosion resistance. These characteristics are mainly attributed to a dual phase and refined microstructure, typical of these alloys, consisting of similar amounts of ferrite and austenite. For this reason, DSS are widely used in various segments of industry, especially in offshore applications. However, DSS components may have a decrease of their properties when they are exposed high temperatures, often due to incorrect manufacturing processes. Intermetallic phases may precipitate causing embrittlement and corrosion resistance decrease. This work presents the effects of short time aging at 475 °C on the mechanical properties and localized corrosion resistance of a cast DSS. The results show that mechanical properties, such as toughness and hardness, are more susceptible to aging, having already been affected during the first 4 hours of heat treatment, while the corrosion was affected in 12 h of aging.