cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7042745
title: Supplementary Material for: In a Nutshell: Findings from the Bruneck Study

description: The Bruneck Study is a prospective community-based study enrolling an age- and sex-stratified random sample of 1,000 men and women. It achieved an extraordinary participation rate of 93.4% and a near-complete long-term follow-up exceeding a quarter of a century (1990–2018). High-quality ascertainment of most common human diseases enables reliable evaluation of: disease epidemiology, overlaps between age-related diseases, and risk factors, and discovery of novel biomarkers. Research priorities include atherosclerosis, cardiovascular disease, aging and longevity, neurological diseases, disorders of the bone, and cancer. This review summarizes the main scientific contributions of the Bruneck Study over the past decades, outlines recent highlights, and gives an outlook of what is planned next.
DOI: 10.6084/m9.figshare.7042715
title: Supplementary Material for: Human Umbilical Cord Blood Therapy Protects Cerebral White Matter from Systemic LPS Exposure in Preterm Fetal Sheep

description: <b><i>Background:</i></b> Infants born preterm following exposure to in utero inflammation/chorioamnionitis are at high risk of brain injury and life-long neurological deficits. In this study, we assessed the efficacy of early intervention umbilical cord blood (UCB) cell therapy in a large animal model of preterm brain inflammation and injury. We hypothesised that UCB treatment would be neuroprotective for the preterm brain following subclinical fetal inflammation. <b><i>Methods:</i></b> Chronically instrumented fetal sheep at 0.65 gestation were administered lipopolysaccharide (LPS, 150 ng, 055:B5) intravenously over 3 consecutive days, followed by 100 million human UCB mononuclear cells 6 h after the final LPS dose. Controls were administered saline instead of LPS and cells. Ten days after the first LPS dose, the fetal brain and cerebrospinal fluid were collected for analysis of subcortical and periventricular white matter injury and inflammation. <b><i>Results:</i></b> LPS administration increased microglial aggregate size, neutrophil recruitment, astrogliosis and cell death compared with controls. LPS also reduced total oligodendrocyte count and decreased mature myelinating oligodendrocytes. UCB cell therapy attenuated cell death and inflammation, and recovered total and mature oligodendrocytes, compared with LPS. <b><i>Conclusions:</i></b> UCB cell treatment following inflammation reduces preterm white matter brain injury, likely mediated via anti-inflammatory actions.
DOI: 10.6084/m9.figshare.7042745.v1
title: Supplementary Material for: In a Nutshell: Findings from the Bruneck Study

description: The Bruneck Study is a prospective community-based study enrolling an age- and sex-stratified random sample of 1,000 men and women. It achieved an extraordinary participation rate of 93.4% and a near-complete long-term follow-up exceeding a quarter of a century (1990–2018). High-quality ascertainment of most common human diseases enables reliable evaluation of: disease epidemiology, overlaps between age-related diseases, and risk factors, and discovery of novel biomarkers. Research priorities include atherosclerosis, cardiovascular disease, aging and longevity, neurological diseases, disorders of the bone, and cancer. This review summarizes the main scientific contributions of the Bruneck Study over the past decades, outlines recent highlights, and gives an outlook of what is planned next.
DOI: 10.6084/m9.figshare.7042715.v1
title: Supplementary Material for: Human Umbilical Cord Blood Therapy Protects Cerebral White Matter from Systemic LPS Exposure in Preterm Fetal Sheep

description: <b><i>Background:</i></b> Infants born preterm following exposure to in utero inflammation/chorioamnionitis are at high risk of brain injury and life-long neurological deficits. In this study, we assessed the efficacy of early intervention umbilical cord blood (UCB) cell therapy in a large animal model of preterm brain inflammation and injury. We hypothesised that UCB treatment would be neuroprotective for the preterm brain following subclinical fetal inflammation. <b><i>Methods:</i></b> Chronically instrumented fetal sheep at 0.65 gestation were administered lipopolysaccharide (LPS, 150 ng, 055:B5) intravenously over 3 consecutive days, followed by 100 million human UCB mononuclear cells 6 h after the final LPS dose. Controls were administered saline instead of LPS and cells. Ten days after the first LPS dose, the fetal brain and cerebrospinal fluid were collected for analysis of subcortical and periventricular white matter injury and inflammation. <b><i>Results:</i></b> LPS administration increased microglial aggregate size, neutrophil recruitment, astrogliosis and cell death compared with controls. LPS also reduced total oligodendrocyte count and decreased mature myelinating oligodendrocytes. UCB cell therapy attenuated cell death and inflammation, and recovered total and mature oligodendrocytes, compared with LPS. <b><i>Conclusions:</i></b> UCB cell treatment following inflammation reduces preterm white matter brain injury, likely mediated via anti-inflammatory actions.
DOI: 10.6084/m9.figshare.7042676
title: Enumerations - Supplementary Data

description: This data accompanies the book, Enumerations: Data and Literary Study (Chicago 2018). All code and other data can be found at https://github.com/piperandrew.
DOI: 10.6084/m9.figshare.7042706
title: Supplementary Material for: Effects of a DPP4 Inhibitor on Progression of NASH-related HCC and the p62/ Keap1/Nrf2-Pentose Phosphate Pathway in a Mouse Model

description: <b><i>Background and Aims:</i></b> Diabetes mellitus is a risk factor for hepatocellular carcinoma (HCC) in patients with nonalcoholic steatohepatitis (NASH). Dipeptidyl peptidase-4 inhibitor (DPP4i), an antidiabetic agent, is reported to affect cell proliferation. We aimed to investigate the effects of DPP4i on the progression of NASH-related HCC and its metabolic pathway in a mouse model. <b><i>Methods:</i></b> A mouse model of NASH-related HCC was used in this study. Eight-week-old mice were administered either DPP4i (sitagliptin 30 mg/kg/day; DPP4i group; <i>n</i> = 8) or distilled water (control group; <i>n</i> = 8) for 10 weeks. Then, HCC progression was evaluated by computed tomography. Changes in metabolites of HCC tissue were analyzed by metabolomic analysis. The localization and expression of p62, Keap1, Nrf2, and MCM7 were evaluated by immunostaining and immunoblotting, respectively. <b><i>Results:</i></b> The number and volume of HCC were significantly lower in the DPP4i group than in the control group (1.8 ± 1.2 vs. 4.5 ± 1.7/liver, <i>p</i> &lt; 0.01; 11.2 ± 20.8 vs. 37.5 ± 72.5 mm<sup>3</sup>/tumor, <i>p</i> &lt; 0.05). Metabolome analysis revealed that DPP4i significantly increased 6-phosphogluconic acid and ribose 5-phosphate levels and decreased the AMP-to-adenine and GMP-to-guanine ratios (AMP-to-adenine ratio 0.7 ± 0.2 vs. 2.0 ± 1.2, <i>p</i> &lt; 0.01; GMP-to-guanine ratio 0.6 ± 0.3 vs. 1.5 ± 0.7, <i>p</i> &lt; 0.01). Immunostaining showed that p62 was localized in the cytoplasm of HCC in the DPP4i group, while p62 was localized in the nucleus of HCC in the control group. Keap1, Nrf2, and MCM7 expression decreased significantly in the DPP4i group compared to that in the control group. <b><i>Conclusions:</i></b> We demonstrated that DDP4i prevented the progression of NASH-related HCC in a mouse model. Furthermore, metabolome analysis revealed that DDP4i downregulated the pentose phosphate pathway with suppression of the p62/Keap1/Nrf2 pathway. Thus, DDP4i may prevent tumor progression through inhibition of metabolic reprogramming in NASH-related HCC.
DOI: 10.6084/m9.figshare.7042649
title: Cost analysis of type 2 diabetes mellitus treatment in economically developed countries

description: <b>Introduction</b>: There is a growing need to evaluate the factors contributing to the increase in health expenditures, as well as the cost of medicines used in the treatment of Type 2 diabetes mellitus (T2DM) in the economically developed countries. <b>Areas covered</b>: A systematic searching of studies describing the direct cost of T2DM and medicine cost of treating T2DM. The quality of the studies found eligible for this study were assessed using a methodological quality appraisal tool. Nine studies were included in this review. All the included studies had data on <i>direct cost</i> and <i>antidiabetic medicine cost</i> of health care. Mean annual direct cost per person/year ranged between US$220 and US$7600. The two components with the greatest impact on direct cost were found to be medicines and hospitalization. The mean annual medicines cost per person/year was found to range between US$140 and US$2990. <b>Expert commentary</b>: There is an increased expenditure on treatment of T2DM. However, it is still not clear how the increased use of antidiabetic medicines is reflected in the overall expenditures. Further, we suggest a more comprehensive understanding of prescription patterns as older drugs are more expensive than the newer drugs, hence they are still under patent.
DOI: 10.6084/m9.figshare.7042619
title: Supplementary Material for: A Dementia Café as a Bridgehead for Community-Inclusive Care: Qualitative Analysis of Observations by On-the-Job Training Participants in a Dementia Café

description: <b><i>Background/Aims:</i></b> Dementia cafés are becoming widespread as a new approach to dementia care, but their operational procedures and significance have not been adequately studied. <b><i>Methods:</i></b> On-the-job training (OJT) for professionals planning to operate a dementia café in the future was conducted in a pioneering dementia café in Japan. The reports of OJT trainees’ observations and learning were analyzed qualitatively. <b><i>Results:</i></b> Reports could be summarized in up to 9 themes: 2 related to the atmosphere and significance of the café, 3 related to the guests, such as people with dementia and their families, and 4 related to the café staff. <b><i>Discussion:</i></b> The results of the present study identified the elements that make up dementia cafés and their significance and suggest that dementia cafés could be a new type of community resource for dementia care in the future.
DOI: 10.6084/m9.figshare.7042649.v1
title: Cost analysis of type 2 diabetes mellitus treatment in economically developed countries

description: <b>Introduction</b>: There is a growing need to evaluate the factors contributing to the increase in health expenditures, as well as the cost of medicines used in the treatment of Type 2 diabetes mellitus (T2DM) in the economically developed countries. <b>Areas covered</b>: A systematic searching of studies describing the direct cost of T2DM and medicine cost of treating T2DM. The quality of the studies found eligible for this study were assessed using a methodological quality appraisal tool. Nine studies were included in this review. All the included studies had data on <i>direct cost</i> and <i>antidiabetic medicine cost</i> of health care. Mean annual direct cost per person/year ranged between US$220 and US$7600. The two components with the greatest impact on direct cost were found to be medicines and hospitalization. The mean annual medicines cost per person/year was found to range between US$140 and US$2990. <b>Expert commentary</b>: There is an increased expenditure on treatment of T2DM. However, it is still not clear how the increased use of antidiabetic medicines is reflected in the overall expenditures. Further, we suggest a more comprehensive understanding of prescription patterns as older drugs are more expensive than the newer drugs, hence they are still under patent.
DOI: 10.6084/m9.figshare.7042556
title: Supplementary Material for: Next-Generation Sequencing Identifies Different Genetic Defects in 2 Patients with Primary Adrenal Insufficiency and Gonadotropin-Independent Precocious Puberty

description: <b><i>Background:</i></b> The development of gonadotropin-independent (peripheral) precocious puberty in male children with primary adrenal insufficiency (PAI) is consistent with a defect in the genes encoding for the enzymes involved in steroid hormone biosynthesis. <b><i>Methods:</i></b> Two young boys presented with peripheral precocious puberty followed by PAI. In both patients, the analysis of <i>CYP21A2</i> gene encoding 21-hydroxylase was normal. As a second step, a targeted next-generation sequencing (NGS) was performed in both patients using a customized panel of congenital endocrine disor ders. <b><i>Results:</i></b> Case 1 had a new homozygous variant in the <i>CYP11B1</i> gene (c.1121+5G&gt;A). Mutations of this gene cause congenital adrenal hyperplasia due to 11β-hydroxylase deficiency, an essential enzyme in the cortisol biosynthesis pathway. Case 2 showed a new hemizygous mutation in the <i>NR0B1</i> gene (c.1091T&gt;G), which encodes for DAX1 (dosage-sensitive sex reversal, adrenal hypoplasia congenita [AHC] and critical region on the X chromosome gene 1). <i>NR0B1</i> mutations cause X-linked AHC<i></i> and hypogonadotropic hypogonadism. Pathogenicity prediction software defined both mutations as probably damaging. <b><i>Conclusions:</i></b> Peripheral precocious puberty was the atypical presentation of 2 rare genetic diseases. The use of NGS made the characterization of these 2 cases with similar clinical phenotypes caused by 2 different genetic defects possible.
DOI: 10.6084/m9.figshare.7042556.v1
title: Supplementary Material for: Next-Generation Sequencing Identifies Different Genetic Defects in 2 Patients with Primary Adrenal Insufficiency and Gonadotropin-Independent Precocious Puberty

description: <b><i>Background:</i></b> The development of gonadotropin-independent (peripheral) precocious puberty in male children with primary adrenal insufficiency (PAI) is consistent with a defect in the genes encoding for the enzymes involved in steroid hormone biosynthesis. <b><i>Methods:</i></b> Two young boys presented with peripheral precocious puberty followed by PAI. In both patients, the analysis of <i>CYP21A2</i> gene encoding 21-hydroxylase was normal. As a second step, a targeted next-generation sequencing (NGS) was performed in both patients using a customized panel of congenital endocrine disor ders. <b><i>Results:</i></b> Case 1 had a new homozygous variant in the <i>CYP11B1</i> gene (c.1121+5G&gt;A). Mutations of this gene cause congenital adrenal hyperplasia due to 11β-hydroxylase deficiency, an essential enzyme in the cortisol biosynthesis pathway. Case 2 showed a new hemizygous mutation in the <i>NR0B1</i> gene (c.1091T&gt;G), which encodes for DAX1 (dosage-sensitive sex reversal, adrenal hypoplasia congenita [AHC] and critical region on the X chromosome gene 1). <i>NR0B1</i> mutations cause X-linked AHC<i></i> and hypogonadotropic hypogonadism. Pathogenicity prediction software defined both mutations as probably damaging. <b><i>Conclusions:</i></b> Peripheral precocious puberty was the atypical presentation of 2 rare genetic diseases. The use of NGS made the characterization of these 2 cases with similar clinical phenotypes caused by 2 different genetic defects possible.
DOI: 10.6084/m9.figshare.7042436
title: Finite element model information and stress results from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: FEA model information and obtained von Mises stress results for all the specimens.
DOI: 10.6084/m9.figshare.7042436.v1
title: Finite element model information and stress results from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: FEA model information and obtained von Mises stress results for all the specimens.
DOI: 10.6084/m9.figshare.7042439
title: Phylogeny from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: Extant platyrrhine phylogeny used in the comparative analyses.
DOI: 10.6084/m9.figshare.7042439.v1
title: Phylogeny from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: Extant platyrrhine phylogeny used in the comparative analyses.
DOI: 10.6084/m9.figshare.7042430
title: Talar 3D coordinates from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: Raw 3D coordinates of the tali that were used to perform all the described morphometric analyses.
DOI: 10.6084/m9.figshare.7042427
title: Convergence procedure applied as part of the Intervals’ method from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: Details of the procedure used to determine the number of intervals to be analysed (i.e., the number of biomechanical variables used in the classification task). The first sheet provides the summary of the applied convergence procedure, while the rest of the sheets provide the values for the different interval variables for all the analysed specimens depending on the number of intervals selected.
DOI: 10.6084/m9.figshare.7042430.v1
title: Talar 3D coordinates from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: Raw 3D coordinates of the tali that were used to perform all the described morphometric analyses.
DOI: 10.6084/m9.figshare.7042427.v1
title: Convergence procedure applied as part of the Intervals’ method from Inferring locomotor behaviours in Miocene New World monkeys using finite-element analysis, geometric morphometrics and machine-learning classification techniques applied to talar morphology

description: Details of the procedure used to determine the number of intervals to be analysed (i.e., the number of biomechanical variables used in the classification task). The first sheet provides the summary of the applied convergence procedure, while the rest of the sheets provide the values for the different interval variables for all the analysed specimens depending on the number of intervals selected.
DOI: 10.6084/m9.figshare.7042049
title: Data from Stier et al. 2019 Sci Rep (Oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress)

description: This excel file contain the raw data used in Stier et al. 2019 Sci Rep to investigate the oxidative stress and mitochondrial responses to stress exposure in king penguin. Details about abbreviations are given in the excel file and information about the physiological parameters in the Table 1 of the original publication.<br>
DOI: 10.6084/m9.figshare.7041971
title: Membrane Spoligotype Mtbc Kinshasa 2005-2010

description: Membrane-based Spoligotyping of retreatment cases in Kinshasa, DRC 2005-2010<br>
DOI: 10.6084/m9.figshare.7041977
title: MIRU-VNTR Mtbc Kinshasa 2005-2010

description: MIRU-VNTR patterns for retreatment cases in Kinshasa, DRC 2005-2010<br>
DOI: 10.6084/m9.figshare.7041971.v1
title: Membrane Spoligotype Mtbc Kinshasa 2005-2010

description: Membrane-based Spoligotyping of retreatment cases in Kinshasa, DRC 2005-2010<br>
DOI: 10.6084/m9.figshare.7041977.v2
title: MIRU-VNTR Mtbc Kinshasa 2005-2010

description: MIRU-VNTR patterns for retreatment cases in Kinshasa, DRC 2005-2010<br>
DOI: 10.6084/m9.figshare.7041977.v1
title: Meehan_Kinshasa_MIRU-VNTR.txt

description: MIRU-VNTR patterns for retreatment cases in Kinshasa, DRC 2005-2010<br>
DOI: 10.6084/m9.figshare.7041929
title: TriSim_RequiredFuns_PRSB.txt from The ecology of movement and behaviour: a saturated tripartite network for describing animal contacts

description: Requisite functions called by the TriSim_Runfile.txt script to generate simulations in Examples 1 and 2.
DOI: 10.6084/m9.figshare.7041932
title: TriSim_Runfile_PRSB.txt from The ecology of movement and behaviour: a saturated tripartite network for describing animal contacts

description: Runfile to generate simulations in Examples 1 and 2.
DOI: 10.6084/m9.figshare.7041929.v1
title: TriSim_RequiredFuns_PRSB.txt from The ecology of movement and behaviour: a saturated tripartite network for describing animal contacts

description: Requisite functions called by the TriSim_Runfile.txt script to generate simulations in Examples 1 and 2.
DOI: 10.6084/m9.figshare.7041932.v1
title: TriSim_Runfile_PRSB.txt from The ecology of movement and behaviour: a saturated tripartite network for describing animal contacts

description: Runfile to generate simulations in Examples 1 and 2.
DOI: 10.6084/m9.figshare.7041392
title: PNTD-D-18-00902_Carrasco-Escobar2018_metadata

description: Metadata regarding number of visits per waterbody and the definition of on-water classes for GEE random forest classification.
DOI: 10.6084/m9.figshare.7041392.v1
title: PNTD-D-18-00902_Carrasco-Escobar2018_metadata

description: Metadata regarding number of visits per waterbody and the definition of on-water classes for GEE random forest classification.
DOI: 10.6084/m9.figshare.7041185
title: Additional file 4: of CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

description: Genbank formatted sequence of repair template pUP613 (Eef1a-FLAG-P2A-eGFP). (TXT 9 kb)
DOI: 10.6084/m9.figshare.7041185.v1
title: Additional file 4: of CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

description: Genbank formatted sequence of repair template pUP613 (Eef1a-FLAG-P2A-eGFP). (TXT 9 kb)
DOI: 10.6084/m9.figshare.7041131
title: Additional file 18: of CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

description: Genbank formatted sequence of repair template pUP660 (Eef1a-SF-P2A-tdTom) (TXT 10 kb)
DOI: 10.6084/m9.figshare.7041131.v1
title: Additional file 18: of CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

description: Genbank formatted sequence of repair template pUP660 (Eef1a-SF-P2A-tdTom) (TXT 10 kb)
DOI: 10.6084/m9.figshare.7041101
title: Additional file 4: of A new bovine tuberculosis model for England and Wales (BoTMEW) to simulate epidemiology, surveillance and control

description: A spreadsheet providing annual numbers of movements recorded in CTS, to indicate the frequency of errors, numbers of transfers between herds during 2008â 2010, and variation among years. (ODS 4 kb)
DOI: 10.6084/m9.figshare.7041101.v1
title: Additional file 4: of A new bovine tuberculosis model for England and Wales (BoTMEW) to simulate epidemiology, surveillance and control

description: A spreadsheet providing annual numbers of movements recorded in CTS, to indicate the frequency of errors, numbers of transfers between herds during 2008â 2010, and variation among years. (ODS 4 kb)
DOI: 10.6084/m9.figshare.7041083
title: Additional file 3: of A new bovine tuberculosis model for England and Wales (BoTMEW) to simulate epidemiology, surveillance and control

description: A spreadsheet file with outputs from a recent run of the anonymised model with observed numbers of breakdowns in 2010 as displayed in Fig. 2. (ODS 115 kb)
DOI: 10.6084/m9.figshare.7041083.v1
title: Additional file 3: of A new bovine tuberculosis model for England and Wales (BoTMEW) to simulate epidemiology, surveillance and control

description: A spreadsheet file with outputs from a recent run of the anonymised model with observed numbers of breakdowns in 2010 as displayed in Fig. 2. (ODS 115 kb)