cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14280176
title: [Hemodynamic and ventilatory parameters in rabbits anesthetized with isoflurane submitted to pressure controlled ventilation with or without PEEP]

description: ABSTRACT Pressure controlled ventilation with or without positive end-expiratory pressure (PEEP) was compared in rabbits, which were divided into three groups denominated GP (pressure cycled ventilation group), GPP (pressure cycled ventilation with PEEP group) and GE (spontaneous ventilation group - control group). The animals were anesthetized with isoflurane in a gas rebreathing circuit for two hours. The means of mean arterial pressure (MAP) and systolic blood pressure (SBP) remained slightly below normal values in all groups. There was a significant decrease in MAP and SBP in the group submitted to PEEP (GPP) over time. The partial pressure of arterial carbon dioxide (PaCO2) was higher in GPP when compared to the other groups, inducing respiratory acidosis after one hour. The end-expired carbon dioxide concentration (ETCO2) presented slightly elevated means in the GP, while the GPP presented higher means, possibly related to the decrease in tidal volume in this group. Based on these results it was concluded that the use of PEEP led to acidemia that worsened over anesthetic time. In addition, prolonged isoflurane anesthesia promotes cardiorespiratory depression, regardless the ventilatory mode employed.
DOI: 10.6084/m9.figshare.14280176.v1
title: [Hemodynamic and ventilatory parameters in rabbits anesthetized with isoflurane submitted to pressure controlled ventilation with or without PEEP]

description: ABSTRACT Pressure controlled ventilation with or without positive end-expiratory pressure (PEEP) was compared in rabbits, which were divided into three groups denominated GP (pressure cycled ventilation group), GPP (pressure cycled ventilation with PEEP group) and GE (spontaneous ventilation group - control group). The animals were anesthetized with isoflurane in a gas rebreathing circuit for two hours. The means of mean arterial pressure (MAP) and systolic blood pressure (SBP) remained slightly below normal values in all groups. There was a significant decrease in MAP and SBP in the group submitted to PEEP (GPP) over time. The partial pressure of arterial carbon dioxide (PaCO2) was higher in GPP when compared to the other groups, inducing respiratory acidosis after one hour. The end-expired carbon dioxide concentration (ETCO2) presented slightly elevated means in the GP, while the GPP presented higher means, possibly related to the decrease in tidal volume in this group. Based on these results it was concluded that the use of PEEP led to acidemia that worsened over anesthetic time. In addition, prolonged isoflurane anesthesia promotes cardiorespiratory depression, regardless the ventilatory mode employed.
DOI: 10.6084/m9.figshare.14280180
title: [Epidemiological analysis of human anti-rabies prophylaxis associated with accidents with cats]

description: ABSTRACT The objective of this study was to know the epidemiological profile of the patient who sought post-exposure anti-rabies treatment associated with accidents with domestic cats in Belo Horizonte, Minas Gerais Brazil, from 2007 to 2016. An exploratory analysis of the reports on human rabies of the Notification of Injury Information System was performed. In the results, it was observed that 37.4% of the individuals were female adults aged 20-59 years, 88.7% were urban residents, 26.1% were white, and only 31.7% sought anti-rabies care within 24 hours of exposure to the rabies virus. Bite lesions occurred in 75.6%. Regarding the type of wound, 59.6% were superficial; as for the anatomical location, 62.2% occurred in hands / feet and 50.1% were single lesions. It is necessary to adopt socio-educational actions among the population, due to the risk of transmission of the rabies by the cat, especially in areas where the rabies virus circulates in bat populations. Improvements in the completion of the SINAN notification sheets are suggested to better understand the epidemiological profile of patients seeking anti-rabies treatment and thus improve the management of this public service.
DOI: 10.6084/m9.figshare.14280180.v1
title: [Epidemiological analysis of human anti-rabies prophylaxis associated with accidents with cats]

description: ABSTRACT The objective of this study was to know the epidemiological profile of the patient who sought post-exposure anti-rabies treatment associated with accidents with domestic cats in Belo Horizonte, Minas Gerais Brazil, from 2007 to 2016. An exploratory analysis of the reports on human rabies of the Notification of Injury Information System was performed. In the results, it was observed that 37.4% of the individuals were female adults aged 20-59 years, 88.7% were urban residents, 26.1% were white, and only 31.7% sought anti-rabies care within 24 hours of exposure to the rabies virus. Bite lesions occurred in 75.6%. Regarding the type of wound, 59.6% were superficial; as for the anatomical location, 62.2% occurred in hands / feet and 50.1% were single lesions. It is necessary to adopt socio-educational actions among the population, due to the risk of transmission of the rabies by the cat, especially in areas where the rabies virus circulates in bat populations. Improvements in the completion of the SINAN notification sheets are suggested to better understand the epidemiological profile of patients seeking anti-rabies treatment and thus improve the management of this public service.
DOI: 10.6084/m9.figshare.14280174.v1
title: 3D printing of canine hip dysplasia: anatomic models and radiographs

description: ABSTRACT Canine Hip Dysplasia (CHD) is a highly prevalent articular pathological condition. In this sense, radiography becomes an important diagnostic method to determine the presence and severity of the disease. The objective was to create 3D models and their respective radiographs representing the CHD (3D AMCHD). The research was carried out in the Laboratory of 3D Educational Technologies of UFAC, under no. 23107.007273/2017-49 (CEUA/UFAC). A canine skeleton (hip bone, femurs and patellae) was used without anatomical deformities compatible with DCF (pelvis, femurs and patella), which were scanned in order to obtain the files of the base model. In these files the deformations representing the different degrees of CHD were performed. Subsequently, the 3D AMCHD files were printed, mounted and X-rayed. The 3D AMCHD represented the bone deformations of the different degrees of CHD. In the radiographs of the 3D AMCHD it was possible to observe and determine each of the bones that constituted the hip joints. This allowed to reproduce the correct positioning to represent the CHD diagnosis and establish the precise points to determine the Norberg angle. In this way, it was evidenced that the 3D AMCHD can be a possible tool to be used in the Teaching of Veterinary Medicine.
DOI: 10.6084/m9.figshare.14280178
title: [Fetal structures and their correlations with a prediction of the parturition date in the miniature canine breeds]

description: ABSTRACT The correlation between fetal and extra-fetal structures with the pre-delivery prediction (DAP) in miniature dog breeds was evaluated. For this, an experiment was carried out using 12 bitches, with body weight between 3.0kg and 5.0kg, being 6 Chihuahua, 2 Shih-Tzu, 2 German Spitz and 2 Yorkshire breed. The Biparietal Diameter (BD), Thoracic Diameter (TD), Abdominal Diameter (AD), Crown-rump length (CRL), Internal Chorionic Cavity Diameter (ICD) and Placenta Thickness (PT) were measured by ultrasonography from the 15th day after the last mating. The simple correlations and significance of simple linear regression coefficients and the coefficient of determination (R) were studied, with a significance level of P&lt;0.05. BD, T, AD and CRL were the most correlated with gestational time, and can be used to predict day before delivery in miniature breed bitches.
DOI: 10.6084/m9.figshare.14280178.v1
title: [Fetal structures and their correlations with a prediction of the parturition date in the miniature canine breeds]

description: ABSTRACT The correlation between fetal and extra-fetal structures with the pre-delivery prediction (DAP) in miniature dog breeds was evaluated. For this, an experiment was carried out using 12 bitches, with body weight between 3.0kg and 5.0kg, being 6 Chihuahua, 2 Shih-Tzu, 2 German Spitz and 2 Yorkshire breed. The Biparietal Diameter (BD), Thoracic Diameter (TD), Abdominal Diameter (AD), Crown-rump length (CRL), Internal Chorionic Cavity Diameter (ICD) and Placenta Thickness (PT) were measured by ultrasonography from the 15th day after the last mating. The simple correlations and significance of simple linear regression coefficients and the coefficient of determination (R) were studied, with a significance level of P&lt;0.05. BD, T, AD and CRL were the most correlated with gestational time, and can be used to predict day before delivery in miniature breed bitches.
DOI: 10.6084/m9.figshare.14280182
title: Epicarditis in a cat caused by feline infectious peritonitis virus: case report

description: ABSTRACT Feline Infectious Peritonitis (FIP) is a progressive and fatal disease in domestic and wild cats, caused by Feline Infectious Peritonitis Virus (FIPV). The disease is characterized by an immunomediated reaction against the virus in various organs. This work described a case report of fibrinous epicarditis caused by FIPV. A male cat, three years old, died and was received to be necropsied. Grossly, soft, multifocal to coalescing, whitish fibrinous exudate, measuring up the 2 centimeters of thickness, was observed in the epicardium, mostly at the apex of the heart. Microscopically, severe, multifocal to coalescing inflammatory infiltrate was observed in the epicardium, composed mainly by macrophages, plasmocytes and lymphocytes, associated with fibrin deposition. Immunohistochemistry was performed for FIPV and was positive in the areas of inflammation in the epicardium. To the author´s knowledge, this is the second report of epicarditis due to FIPV in a cat. Therefore, epicarditis should be considered a differential diagnosis of cardiac diseases in Feline Medicine.
DOI: 10.6084/m9.figshare.14280182.v1
title: Epicarditis in a cat caused by feline infectious peritonitis virus: case report

description: ABSTRACT Feline Infectious Peritonitis (FIP) is a progressive and fatal disease in domestic and wild cats, caused by Feline Infectious Peritonitis Virus (FIPV). The disease is characterized by an immunomediated reaction against the virus in various organs. This work described a case report of fibrinous epicarditis caused by FIPV. A male cat, three years old, died and was received to be necropsied. Grossly, soft, multifocal to coalescing, whitish fibrinous exudate, measuring up the 2 centimeters of thickness, was observed in the epicardium, mostly at the apex of the heart. Microscopically, severe, multifocal to coalescing inflammatory infiltrate was observed in the epicardium, composed mainly by macrophages, plasmocytes and lymphocytes, associated with fibrin deposition. Immunohistochemistry was performed for FIPV and was positive in the areas of inflammation in the epicardium. To the author´s knowledge, this is the second report of epicarditis due to FIPV in a cat. Therefore, epicarditis should be considered a differential diagnosis of cardiac diseases in Feline Medicine.
DOI: 10.6084/m9.figshare.14280188
title: Modified comet assay with Giemsa staining: a suitable method for assessing genotoxicity in rats

description: ABSTRACT The present study tested a comet assay that was modified for compatibility with Giemsa staining to assess the drug genotoxicity in the peripheral blood of rats. We analysed the peripheral blood of 16 female Wistar rats (N=8 rats/group) from a control group and from a group that was treated with an intraperitoneal injection of 50mg cyclophosphamide/kg. The comet assay was carried out with modifications of the blood volume and immersion time in the lysing solution and different combinations of electrophoresis conditions (running time, voltage and current), to Giemsa staining. The lysing time and electrophoresis conditions allowed for the expression of all classes of DNA damage during the electrophoresis run, and the comets were efficiently stained with Giemsa. The technique showed high reproducibility for the DNA classes. The results demonstrate that the modified comet assay with Giemsa staining can be standardized for routine laboratory procedures using a 20µL blood sample, 3h and 30min immersions in the lysing solution and electrophoresis runs with 23 to 25 V and 310 and 360mA of electrical current. The modified comet assay with Giemsa staining that was described in the present study was standardized to be applied in the laboratory routine.
DOI: 10.6084/m9.figshare.14280188.v1
title: Modified comet assay with Giemsa staining: a suitable method for assessing genotoxicity in rats

description: ABSTRACT The present study tested a comet assay that was modified for compatibility with Giemsa staining to assess the drug genotoxicity in the peripheral blood of rats. We analysed the peripheral blood of 16 female Wistar rats (N=8 rats/group) from a control group and from a group that was treated with an intraperitoneal injection of 50mg cyclophosphamide/kg. The comet assay was carried out with modifications of the blood volume and immersion time in the lysing solution and different combinations of electrophoresis conditions (running time, voltage and current), to Giemsa staining. The lysing time and electrophoresis conditions allowed for the expression of all classes of DNA damage during the electrophoresis run, and the comets were efficiently stained with Giemsa. The technique showed high reproducibility for the DNA classes. The results demonstrate that the modified comet assay with Giemsa staining can be standardized for routine laboratory procedures using a 20µL blood sample, 3h and 30min immersions in the lysing solution and electrophoresis runs with 23 to 25 V and 310 and 360mA of electrical current. The modified comet assay with Giemsa staining that was described in the present study was standardized to be applied in the laboratory routine.
DOI: 10.6084/m9.figshare.14280185
title: Sanitary conditions of the Goliath grouper Epinephelus itajara in captivity on estuarine environment: case report

description: ABSTRACT This study aimed to report the sanitary conditions through the hematological analysis of grouper E. itajara reared in captivity on estuarine conditions. Seven Goliath groupers (1,881.5±1,246.03g) were captured and kept in two tanks located on estuary. After 20 days, fish were collected for morphologic and hemato-physiologic evaluation. Two fish had clinical signs such as hemorrhagic spots and loss of scale due to agonistic behavior. Blood samples were collected, and the hematological parameters (biochemical, erythrogram and leukogram) were determined. Blood cells were characterized by their size, color and shape. Univariate statistic and principal components analysis were used to identify a hematological standard between fish with or without clinical signs. Four leukocyte types were found: lymphocyte, monocyte, neutrophil and basophil. Regardless of the clinical signs the cell morphology did not present any difference among the fish. However, there is a significant correlation between erythrocyte and lactate on fish with clinical signs. Thus, agonistics encountered among the fish is a stressing factor in captivity conditions making it necessary to have adequate management related to the size of fish and stocking density.
DOI: 10.6084/m9.figshare.14280183.v1
title: Saccharomyces cerevisiae as a probiotic agent and a possible aflatoxin B1 adsorbent in simulated fish intestinal tract conditions

description: ABSTRACT The aim of this study was to evaluate in vitro the probiotic potential and absorption of Saccharomyces cerevisiae for the aflatoxin B1 in simulated fish intestinal tract conditions. Three yeast strains were used, two from brewery: S. cerevisiae RC1 and S. cerevisiae RC3 and one from a fish farming environment: S. cerevisiae A8L2. The selected yeasts were subjected to the following in vitro tests: homologous inhibition, self-aggregation, co-aggregation, antibacterial activity, gastrointestinal conditions tolerance and adsorption of AFB1. All S. cerevisiae strains showed good capability of self-aggregation and co-aggregation with pathogenic bacteria. All yeast strains were able to survive the gastrointestinal conditions. In acidic conditions, the factors (strain vs. time) had interaction (P=0.0317), resulting in significant variation among the strains tested in the time periods analyzed. It was observed that there was also interaction (P=0.0062) in intestinal conditions, with an increased number of cells in the 12-hour period for all strains tested. In the adsorption test, the A8L2 strain was statistically more effective (P&lt;0.005) for both AFB1 concentrations evaluated in this study (10 and 25ng/mL). Thus, it was observed that the strains of S. cerevisiae have potential probiotic and adsorbent of AFB1.
DOI: 10.6084/m9.figshare.14280185.v1
title: Sanitary conditions of the Goliath grouper Epinephelus itajara in captivity on estuarine environment: case report

description: ABSTRACT This study aimed to report the sanitary conditions through the hematological analysis of grouper E. itajara reared in captivity on estuarine conditions. Seven Goliath groupers (1,881.5±1,246.03g) were captured and kept in two tanks located on estuary. After 20 days, fish were collected for morphologic and hemato-physiologic evaluation. Two fish had clinical signs such as hemorrhagic spots and loss of scale due to agonistic behavior. Blood samples were collected, and the hematological parameters (biochemical, erythrogram and leukogram) were determined. Blood cells were characterized by their size, color and shape. Univariate statistic and principal components analysis were used to identify a hematological standard between fish with or without clinical signs. Four leukocyte types were found: lymphocyte, monocyte, neutrophil and basophil. Regardless of the clinical signs the cell morphology did not present any difference among the fish. However, there is a significant correlation between erythrocyte and lactate on fish with clinical signs. Thus, agonistics encountered among the fish is a stressing factor in captivity conditions making it necessary to have adequate management related to the size of fish and stocking density.
DOI: 10.6084/m9.figshare.14280189
title: [Ultrastructural analysis of the collagen of rabbit skin wounds treated with platelet-rich equine plasma]

description: ABSTRACT Collagen is synthesized and secreted into the extracellular space and organized into striated fibrils according to the tissue type. This study evaluated the concentration of collagen in rabbit skin wounds treated with equine platelet-rich plasma (PRP) and ointment containing gentamicin, sulfanilamide, sulfadiazine, urea, and vitamin A. Twenty-four New Zealand white rabbits aged 2 to 12 months and weighing 3.0kg were included. The animals were allocated equally into four groups and the skin was removed from the thoracic dorsal midline (treated wound) and lumbar (control wound) regions. The thoracic wounds were treated with equine PRP and ointment containing gentamicin, sulfanilamide, sulfadiazine, urea, and vitamin A, and the control group was treated with the ointment alone. For histological and ultrastructural assessment, cutaneous tissue was collected on postoperative days 3 (group I), 7 (group II), 14 (group III), and 21 (group IV). After the evaluation period, in each group, a skin fragment was collected for analysis of the collagen concentration, as well as the collagen fibril diameter and density by transmission electron microscopy. The results indicated that treatment with equine PRP combined with topical application of the ointment was effective in facilitating the maturation of collagen fibrils and the wound healing process.
DOI: 10.6084/m9.figshare.14280189.v1
title: [Ultrastructural analysis of the collagen of rabbit skin wounds treated with platelet-rich equine plasma]

description: ABSTRACT Collagen is synthesized and secreted into the extracellular space and organized into striated fibrils according to the tissue type. This study evaluated the concentration of collagen in rabbit skin wounds treated with equine platelet-rich plasma (PRP) and ointment containing gentamicin, sulfanilamide, sulfadiazine, urea, and vitamin A. Twenty-four New Zealand white rabbits aged 2 to 12 months and weighing 3.0kg were included. The animals were allocated equally into four groups and the skin was removed from the thoracic dorsal midline (treated wound) and lumbar (control wound) regions. The thoracic wounds were treated with equine PRP and ointment containing gentamicin, sulfanilamide, sulfadiazine, urea, and vitamin A, and the control group was treated with the ointment alone. For histological and ultrastructural assessment, cutaneous tissue was collected on postoperative days 3 (group I), 7 (group II), 14 (group III), and 21 (group IV). After the evaluation period, in each group, a skin fragment was collected for analysis of the collagen concentration, as well as the collagen fibril diameter and density by transmission electron microscopy. The results indicated that treatment with equine PRP combined with topical application of the ointment was effective in facilitating the maturation of collagen fibrils and the wound healing process.
DOI: 10.6084/m9.figshare.14280193
title: [Microcystic meningioma with pulmonary metastasis in canine: case report]

description: ABSTRACT Meningiomas are the main tumors of the central nervous system (CNS) affecting dogs and cats. In most of the cases they are benign neoplasms, usually expansive, causing compression of the CNS and rarely metastasize to other organs. We describe the occurrence of a microcystic meningioma with pulmonary metastasis in an 11 - year - old canine with clinical signs of staggering gait, head compression against objects, agitation, salivation and aggressiveness. At necropsy, well-defined, firm, granular-looking masses located in the parietal cortex and nuclei of the base were observed in the encephalon. Numerous micronodulations of similar appearance were observed in the lung. Histologically, nodules formed by spindle neoplastic cells with large, elongated nuclei and evident nuclei were loosely arranged, forming vacuoles and microcysts. Immunohistochemistry were strongly positive for cytokeratin and negative for vimentin. Through the histopathology and immunohistochemistry, it was possible to establish the histological classification of microcystic meningioma, as well as to differentiate from other diseases that present with nervous signals.