cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14288561.v1
title: COLD STORAGE OF TRICHOGRAMMA PRETIOSUM RILEY (HYMENOPTERA: TRICHOGRAMMATIDAE)

description: ABSTRACT The objective of this work was to evaluate the effect of storage at low temperature of Trichogramma pretiosum pupae in eggs of Sitotroga cerealella on the emergence and reproduction rates of the parasitoid. The trials were conducted in a climatic chamber at 5, 8 and 10º C, 70% R.H., constant scotophase, with 10 treatments: 4, 6, 8, 10, 12, 14, 16, 18, 20 days of storage and a control not kept in storage, all in 10 replicates. A total of 1,000 eggs containing pupae were distributed in glass tubes (10 x 3 cm), 100 of which were kept at 25° C, 70% RH and 14 h photophase (control), while the remaining ones were stored. After each storage period 100 eggs were transferred to 25º C and the emergence and reproductive capacity were observed. The T. pretiosum emergence rate was not affected by the storage time at 3 temperatures (Tukey P ≤ 0.05) and the lowest emergence rates were 85.6%, 84.5% and 77.6%, respectively for parasitoid storage at 5, 8 and 10º C. The reproductive capacity of T. pretiosum was not affected after the exposure periods in the temperatures evaluated. This result will aid in the planning of mass production, package and transport of T. pretiosum to the place of release.
DOI: 10.6084/m9.figshare.14288558.v1
title: EFFECT OF SILICON ON THE INTENSITY OF BROWN EYE SPOT AND ON THE MINERAL NUTRITION OF COFFEE SEEDLINGS

description: ABSTRACT Our objective was to verify the effect of silicon on the intensity of brown eye spot and on the mineral nutrition of coffee seedlings. In the first experiment, 6 doses of silicic acid (0, 0.5, 1, 2, 4 and 6 g kg-1 soil) were tested using a complete randomized block design with 4 replicates with 6 coffee seedlings cultivar Catuaí Vermelho IAC 99 inoculated with the fungus Cercospora coffeicola. In the second experiment, X-ray microanalysis in a scanning electron microscope was performed on 2 coffee cultivars (Topazio MG1190 and Icatu Precoce IAC 3282), inoculated and non-inoculated with C. coffeicola, treated and untreated with calcium silicate (1 g kg-1 of soil). With the increase of the silicic acid doses, there was observed a reduction in the area under the disease progress curve of the number of lesions per leaf (AUPCNLL), coupled with a linear reduction in the foliar contents of magnesium and phosphorus as well as an increase in the contents of sulfur and copper. The foliar contents of boron presented a quadratic behavior, decreasing with the increase of silicic acid and increasing with the dose of 4 g kg-1 of soil. In X-ray microanalysis, coffee seedlings with brown eye spot presented lower peaks of potassium and calcium, regardless of the cultivar used.
DOI: 10.6084/m9.figshare.14288557.v1
title: REPRODUCTIVE ORGANS OF SPARTOCERA DENTIVENTRIS (BERG, 1884) (HEMIPTERA: COREIDAE) AT DIFFERENT AGES

description: ABSTRACT In this work, the internal reproductive organs of Spartocera dentiventris males and females with different ages are described, measured and illustrated. The most significant changes in the reproductive organs were found in the length and width of ovaries, which permit the distinction between immature and reproductive stages. No significant difference was found among the measurements of the reproductive structures of males at the different ages.
DOI: 10.6084/m9.figshare.14288556.v1
title: MORPHOMETRY OF ANASTREPHA FRATERCULUS (WIED) (DIPTERA: TEPHRITIDAE) RELATED TO NATIVE MYRTACEAE HOSTS

description: ABSTRACT The fruit-fly Anastrepha fraterculus uses the myrtacean fruits as its main primary hosts. The insect size might be an indicator of aptitude and fecundity, and morphometric studies are used to help in those identifications. This study aimed to verify the association among fruit weight, puparial weight and wing size of fruit-flies that developed in different myrtacean species. Yellow strawberry guava, cattle guava, guava and feijoa fruits were sampled from 5 trees of each species. The fruits were weighed, and the puparia originated from them were also weighed and individualized. The number, gender and total right-wing area of the flies were registered. Mean fruit and puparial weights and wing sizes were compared. Mean puparial weight was higher in guava and cattle guava. The largest mean wing area was recorded for individuals from guavas; the smallest, for those from yellow strawberry guava. The fruit and puparial weights did not present a significant association in yellow strawberry guava, cattle guava and feijoa. In guavas, the correlation between fruit and puparial weights was negative. The fruit weight and wing area in all myrtacean species did not show any correlation. These results indicate the fly is able to develop similarly in all sampled myrtacean species.
DOI: 10.6084/m9.figshare.14288553.v1
title: EVALUATION OF OREGANO AND ROSEMARY ESSENTIAL OILS FOR THE PREVENTION OF NEONATAL DIARRHEA IN PIGLETS

description: ABSTRACT In order to verify the preventive effect of the liquid emulsion of a mix of essential oils of oregano (Origanum vulgare) and rosemary (Rosmarinus officinalis) in the control of neonatal diarrhea in nursing piglets, an experiment was conducted using a randomized experimental design composed by two simple treatments. Piglets from the same litter, born to females with different orders of births, were divided into two groups of piglets without clinical signs of neonatal diarrhea. The first group of 661 pigs received the control treatment (1 mL kg-1 saline) while the second group with 672 animals received essential oils of oregano (60% carvacrol and 15% thymol) and 2% essential oil of rosemary in the form of a 5% liquid emulsion at a dose of 1 mL kg-1. All the treatments were administered orally on the first and third day of the piglets’ life, and in regard to the index parameter for the prevention and causes of mortality of piglets, each animal represented a repetition. There was no significant difference between the treatments related to the rate of prevention of neonatal diarrhea in piglets. The results showed that the mix of essential oils from oregano and rosemary do not prevent diarrhea in neonatal piglets.
DOI: 10.6084/m9.figshare.14288552.v1
title: CONSERVATION OF NATURAL ENEMIES (INSECTA) IN THE ORGANIC TOMATO CROP

description: ABSTRACT The adoption of cultural practices such as companion plants and the kind of irrigation system can benefit the community of natural enemies in the agroecosystem due to the increase of more favorable microhabitats available and as a source of alternative resources, mainly in periods of low precipitation. This study evaluated how the tomato + coriander intercrop and the kind of irrigation (drip and sprinkler) could benefit the community of natural enemies in the tomato agroecosystem. This work was carried out in the experimental field of Embrapa Hortaliças, Gama, DF, from September to November 2008. The tomatoes were planted in monoculture or with coriander (companion plant) and irrigated by drip and sprinkler irrigation (three replicates per treatment), forming two groups of experimental plots according to the kind of irrigation. The community of natural enemies was sampled by the direct observation of specimens on 20 tomatoes plants per plot and in the treatments. Coriander plants were shaken over a plastic tray for collection of insects. The abundance, richness and diversity of natural enemies were higher in tomato + coriander plots, regardless of the irrigation system. We observed more species of natural enemies in tomato monoculture plots when it was irrigated by sprinkler irrigation. However, the cultural practices adopted had a different effect on each species or group of species. Therefore, in periods of low precipitation, the tomato + coriander consortium associated with sprinkler irrigation can enhance the conservation of natural enemies in the organic tomato agroecosystem.
DOI: 10.6084/m9.figshare.14288549.v1
title: ANTIMICROBIAL SUSCEPTIBILITY OF SAMPLES OF ESCHERICHIA COLI ISOLATED FROM INDUSTRIAL CHICKENS (GALLUS GALLUS DOMESTICUS LINNAEUS, 1758) WITH COLIBACILLOSIS

description: ABSTRACT The present study evaluated the susceptibility of Escherichia coli samples isolated from poultry with colisepticemia in midwestern São Paulo State at the Ornitopathology Laboratory of FMVZ-UNESP/Botucatu, SP. A large number of samples were found that were multi-resistant to the antibiotics tested, the less effective drugs being sulfonamide and tetracycline. All samples were susceptible to norfloxacin and gentamicin.
DOI: 10.6084/m9.figshare.14288547.v1
title: ASSESSMENT OF INTERCURRENCE OF ENZOOTIC LEUCOSIS AND BOVINE TUBERCULOSIS IN DAIRY COWS IN THE STATE OF PERNAMBUCO, BRAZIL

description: ABSTRACT We carried out this study to evaluate the intercurrence of tuberculosis and enzootic leukosis in dairy cattle herds in 8 districts of the State of Pernambuco, Brazil, by establishing the prevalence of bovines reagent to specific diagnostic tests, and to also verify the correlation between enzootic leucosis, cattle tuberculosis, the leukocytes count and lymphocytes count among the cattle studied. A total of 662 animals were tested serologically and hematologically, by the techniques of double immunodiffusion in agarose gel and the white blood cell count with the total and differential leukocyte count, respectively, and 612 were evaluated immunoallergically by the tuberculin test. In the 15 herds examined, the prevalence of cattle that were positive to the AGID and tuberculin tests were 32.2% (213/662) and 14.1% (86/612), respectively. Analyzing the correlation between the variables total leukocytes and lymphocytes, we found that total leukocyte is highly related to the variable lymphocytes (72%). The results in this study indicated that the diseases studied are widespread in the population studied, with significant levels of growth in leukemia, and infections of bovine leukosis virus (VLB) and Mycobacterium bovis are active and growing, with risk of impairment of the health of cattle herds, and, due to the zoonotic nature of bovine tuberculosis, of the health of people who work with cattle.
DOI: 10.6084/m9.figshare.14288546.v1
title: FRUIT FLIES (DIPTERA: TEPHRITIDAE), THEIR PARASITOIDS AND HOSTS IN VIÇOSA, A FOREST ZONE IN THE STATE OF MINAS GERAIS, BRAZIL

description: ABSTRACT This paper presents the species diversity of fruit flies (Tephritidae), their parasitoids and hosts in the county of Viçosa, Minas Gerais State. Brazil. McPhail traps containing hydrolyzed protein were hung in three areas with several crop species, and in a natural reserve of the Atlantic Rain Forest. Ripe fruits on plants and fallen fruits were collected to obtain fruit fly and parasitoid adults. Sixteen fruit fly species were captured: Ceratitis capitata (Wiedemann), Anastrepha bezzi Lima, A. bistrigata Bezzi, A. dissimilis Stone, A. distincta Greene, A. fraterculus (Wiedemann), A. furcata Lima, A. grandis (Macquart), A. manihoti Lima, A. minensis Lima, A. montei Lima, A. obliqua (Macquart), A. pseudoparallela (Loew), A. pickeli Lima, A. serpentina (Wiedemann) and A. sororcula Zucchi. A. furcata is reported for the first time in Minas Gerais state. Furthermore, the parasitoids Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan, Utetes anastrephae (Viereck) (Braconidae) and Aganaspis pelleranoi (Brèthes) (Figitidae) were obtained, along with 4 specimens of Pteromalidae. This is the first report of these parasitoids in the region of Viçosa, and of O. bellus in Minas Gerais State.
DOI: 10.6084/m9.figshare.14288544.v1
title: DIVERSITY AND CONTROL OF PEST INSECTS IN THE JUDICIAL ARCHIVE OF THE CITY COUNCIL OF PITANGUI, STATE OF MINAS GERAIS, BRAZIL

description: ABSTRACT Destruction of books and documents by pest organisms is an age-old problem, faced mainly by both librarians and archivists. The present study provides a brief report on the main actions and the results of the Integrated Management of Pests (IMP) implemented in the Judicial Archive of the City Council of Pitangui, state of Minas Gerais, Brazil, during the months of January 2004 to August 2005. These actions included a survey of the pest organisms present both in the physical area of the archive and in the bibliographical material. All the material was treated through the controlled freezing method, which consistsin putting the bibliographical material into a freezer at negative temperatures for a predetermined time period. In the case of this method, the effectiveness of the control was evaluated through the verification of the presence of dead and living individuals, soon after the treatment and at three and six months later. The survey of the pest organisms in the physical area of the archive revealed the presence of the termite Cryptotermes brevis (Walker) and the ants Tapinoma melanocephalum (Fabricius) and Camponotus sp. In the bibliographical material, in addition to the termite C. brevis there were identified beetles of the species Tricorynus herbarius (Gorham), silverfish Lepisma saccharina Linnaeus, and book lice of the genus Liposcelis sp. The control measures evidenced the effectiveness of the methods used, with no reoccurrence of the pest during the study period.
DOI: 10.6084/m9.figshare.14288543.v1
title: DEVELOPMENT OF PERIPLANETA AUSTRALASIAEFABRICIUS (BLATTODEA, BLATTIDAE) AT A CONSTANT TEMPERATURE OF 30° C AND AT AMBIENT LABORATORY TEMPERATURE

description: ABSTRACT With the objective of obtaining standards of measurement for prevention and control, this study compared the development of the eggs and nymphs of Periplaneta australasiae (Fabricius) at a temperature of 30 ± 0.2° C, relative humidity 80 ± 15% and photoperiod of 12 hours versus ambient conditions in the laboratory without controls of temperature and RH. Single ootheca were maintained in test tubes until ecolosion, and nymphs were transferred to glass cubes and fed commercial rabbit ration and water ad libitum until emergence of the imagos. The incubation period, number of eggs/ ootheca, viability of the eggs, number of nymphs/ootheca, nymphal duration, viability of nymphs and duration of egg to adult were all evaluated. The mean difference in the incubation period between the temperature of 30° C (38 days) and ambient conditions (44.5 days) was significant (p &lt; 0.0001); a mean of 18.1 nymphs/ootheca ecloded at 30° C, while 21 nymphs/ootheca ecloded under ambient conditions (p = 0.006); the mean nymphal period at 30° C was 155.9 days while for the ambient it was 279.7 days (p &lt; 0.0001); nymphal viability was greater than 50% for both the 30° C laboratory (55.1%) and the ambient (57.2%); and the mean period from egg to adult of P. australasiae was significantly different (p &lt; 0.001) between the 30° C temperature (194.1 days) and the ambient conditions of the laboratory (337.3 days). Under ambient conditions, the duration of nymphal incubation and egg to adult development of P. australasiae were increased relative to the temperature of 30° C without a reduction in viability in any of the stages.
DOI: 10.6084/m9.figshare.14288541.v1
title: SUSCEPTIBILITY OF RHYNCHOPHORUS PALMARUM TO THE ACTION OF METARHIZIUM ANISOPLIAE AND THE COMPATIBILITY OF THE ENTOMOPATHOGEN WITH AGROCHEMICALS USED IN THE BANANA CROP

description: ABSTRACT This study was aimed to screen the entomopathogenic fungus Metarhizium anisopliae for the control of Rhynchophorus palmarum (L.) (Coleoptera: Curculionidae) adults and to evaluate the compatibility of agrochemicals used in this crop with the strain that caused the highest insect mortality. To this end, adult insects were immersed in suspensions of 6 strains at a concentration of 109 conidia.mL-1, and after 24 days the isolate IBCB 348 caused the highest mortality (87.5%) and was used in the compatibility test, using calculations by way of the T formula, with commercial insecticides, herbicides and fungicides used in the crop. Among the tested strains, IBCB 348 was the most efficient in the control of this pest, causing 87.5% of weevil mortality. None of the tested fungicides and herbicides were compatible with the pathogen. The insecticides Calypso, Dipterex 500 and Sevin 480 SC, at the lowest dose recommended by the manufacturer, were the only products classified as compatible with the strain IBCB 348 of M. anisopliae.
DOI: 10.6084/m9.figshare.14288540.v1
title: SELECTION AND MOLECULAR CHARACTERIZATION OF BACILLUS THURINGIENSIS BERLINER WITH TOXIC ACTIVITY AGAINST TRICHOPLUSIA NI HUEBNER (LEPIDOPTERA: NOCTUIDAE)

description: ABSTRACT Trichoplusia ni is a polyphagous pest that is becoming a major pest in plantations of cruciferous crops, soybeans and cotton. This study was aimed to select and molecularly characterize efficient isolates of Bacillus thuringiensis (Bt) for the control of T. ni. For the bioassays of pathogenicity, an aliquot with a 3 x 108 spores/mL suspension of Bt of each isolate was applied on the surface of the artificial diet disk, previously distributed on acrylic plates with 50 larvae, distributed in 5 repetitions. In bioassays to obtain the LC50, only isolates with 100% mortality were preselected, and tests were carried out at the concentrations 102, 5 x 102, 103, 2 x 103, 4 x 103, 6 x 103, and 8 x 103 spores/mL, and the treatments consisting of 120 larvae, distributed in 3 repetitions. A molecular characterization was performed to detect the genes cry1, cry2 and Vip for the isolates which obtained mortality over 95%. Isolates HD-1 (Standard), Bt-1043N-V, Bt-1034F, Bt-1009K, Bt-1000 and Bt-969A caused 100% mortality in the test for pathogenicity and presented an LC50 of 1.17 x 103, 1.45 x 103, 1, 46 x 103, 1.01 x 103, 9.43 x 102, 1.22 x 103, respectively. Genes cry1, cry2 and Vip were not found in the isolates tested, and other Cry toxins may have been causing the mortality of T. ni, since the isolates tested are specific for the Lepidoptera order. These isolates showed potential for the control of T. ni, being aggressive to this insect, with a potential to be used in a pest management program for this species.
DOI: 10.6084/m9.figshare.14288537.v1
title: SEMI-SOLID PRODUCTION OF METARHIZIUM ANISOPLIAE (METSCH.) SOROK AND BEAUVERIA BASSIANA (BALS.) VUILL ON DIFFERENT MEDIA AND THE EFFECTS OF ULTRAVIOLET RADIATION AND TEMPERATURE ON THE INFECTIVE PROPAGULES OF THESE FUNGI

description: ABSTRACT The present study was aimed to evaluate different (semi-solid) media for the production of Metarhizium anisopliae and Beauveria bassiana propagules, and to evaluate the tolerance of these propagules to ultraviolet radiation and temperature. The experiments were performed at the Biological Control Laboratory of the Instituto Biológico at Campinas, São Paulo, Brazil. For both fungi, 6 repetitions were performed for each of the 17 treatments: corn starch, full rice, parboiled rice, type-1 rice, type-2 rice, oat flakes, canjiquinha [grits], wheat flour, raw cassava flour, yellow corn flour, special wheat flour, corn flour, corn in grains, cassava starch, soy in grains, crushed wheat, and turf. The viability analysis was done in plastic plates containing BDA. For the bioassays involving exposure to ultraviolet light and temperature, BDA was also used for viability analysis, and each treatment was exposed to the UV radiation for 0, 25 and 50 seconds, the temperature exposure being at 20, 25, 30 and 35º C. Using a Potter tower, 2 mL of fungus suspension from each treatment was inoculated to the Diatraea saccharalis caterpillars. Regarding the sporulation, the largest concentrations of M. anisopliae and B. bassiana were found for the treatments with parboiled rice, type-1 rice, type-2 rice, yellow corn flour, corn flour and crushed wheat. The viability of all treatments was superior to 94.00%. Also, the longer the duration of the exposition to the UV, the 2Universidade Estadual Paulista, Faculdade de Ciências Agronômicas, Botucatu, SP, Brasil.
DOI: 10.6084/m9.figshare.14288536.v1
title: EFFECTIVENESS OF A NEW COMMERCIAL INSECTICIDE FOR THE CONTROL OF THE LESSER MEALWORM (ALPHITOBIUS DIAPERINUS) (PANZER) (COLEOPTERA: TENEBRIONIDAE)

description: ABSTRACT The present study was carried out with the objective to evaluate, under laboratory and field conditions, the effectiveness of the insecticide Test (based on cypermethrin, chlorpyrifos and citronellal), for the control of lesser mealworm (Alphitobius diaperinus). In the laboratory, the product was tested on adult insects, by spraying on the insects (direct contact) and by their walking on a treated surface (indirect contact). After this, it was evaluated in field conditions, in a poultry house. The product was effective, being comparable to the standard product, causing 100% of mortality in the laboratory and reducing the population in the poultry house by 76%. Thus, the product presents an alternative for the control of this pest.
DOI: 10.6084/m9.figshare.14288534.v1
title: PRODUCTIVE AND SANITARY ASPECTS OF CROSSBRED MILKING COWS RECEIVING HOMEOPATHIC PRODUCTS

description: ABSTRACT Nowadays the world population demands food of good quality, without any residues from chemical products. Eight cows from a herd of 40, which were consuming commercial homeopathic medicines at the daily concentration for control of endoand ectoparasites, were monitored in terms of their performance (production and quality of milk, body score and body weight) and sanitary aspects (parasites infestation and mastitis) during the lactation period. It was not necessary to give any allopathic (conventional) medicines to control mastitis or parasites (ticks, horn-fly, verminosis) in any cow of the herd during the nine months of the lactation period. The results showed that it is possible to produce milk without using chemical products, and not interfering in the productivity of the crossbred animals.
DOI: 10.6084/m9.figshare.14288532.v1
title: CLONING AND EXPRESSION IN PICHIA PASTORIS OF THE S1 GLYCOPROTEIN GENE OF THE CHICKEN INFECTIOUS BRONCHITIS VIRUS

description: ABSTRACT Genetic and antigenic variation are very frequently observed among IBV strains and affect mainly the S1 glycoprotein. In order to contribute to the availability of tools for immunodiagnosis and immunoprophylaxis of chicken infectious bronchitis we developed an expression system for production of recombinant S1 glycoprotein in Pichia pastoris. We obtained the cDNA from viral RNA on embryonated eggs infected with the M41 strain of IBV, by reverse transcription (RT) and polymerase chain reaction (PCR), amplifying the S1 coding sequence with extremities compatible with the vector used to transform yeast. Induction with methanol led to the production of a protein with the predicted molecular weight that was detected by Western blot in the cell lysate of transformed yeast. Expression in P. pastoris proved to be an effective method for recombinant production of S1 protein from IBV, with potential for use in immuno-diagnosis of chicken infectious bronchitis virus.
DOI: 10.6084/m9.figshare.14288530.v1
title: IN VITRO EFFECT ON PHYTOPATHOGENS OF ESSENTIAL OIL AND AQUEOUS CRUDE EXTRACT OF OCIMUM GRATISSIMUM HARVESTED IN THE FOUR SEASONS

description: ABSTRACT The present &gt;work aimed to evaluate the effects of the essential oil and crude extract of wild basil (Ocimum gratissimum), harvested in different seasons, on the in vitro mycelia growth of Rhizoctonia solani, Sclerotium rolfsii, Phytophthora sp. and Alternaria alternata. To evaluate the effect of the essential oil, sterilized oil aliquots of 20 ?L, 40 ?L and 60 ?L were distributed on the surface of potato-dextroseagar (PDA). The aqueous crude extracts (ACE) were filtrated and incorporated in PDA at 1%, 5%, 10%, 15%, 20%, 25% and 50%. The results showed total inhibition of mycelial growth in the different aliquots of essential oil. The ACE collected from plants in autumn had better results; in this season the ACE at 5% was enough to provide 100% of mycelia growth inhibition of A. alternata and S. rolfsii.