cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7420232
title: Feed additives can differentially modulate NF-κB (RelA/p65), IGF-1, GLUT2, and SGLT1 gene expression in porcine jejunal explants

description: ABSTRACT The intestinal gene expression of RelA/p65 (NF-κB), insulin growth factor 1 (IGF-1), glucose transporter 2 (GLUT2), and Na+/dependent glucose transporter 1 (SGLT1) were evaluated in response to benzoic acid, yeast culture, L-glutamine, and oregano essential oil, using an ex vivo model. Six piglets weighing approximately 20 kg each were sacrificed, and their jejunum was collected and segmented into five 2-cm explants. Each explant was immersed in cell culture medium according to one of the following treatments: control (without additive), 0.5% benzoic acid, 1% yeast culture, 1% L-glutamine, and 0.015% oregano oil. Gene expression was evaluated using RT-PCR. Yeast culture up-regulated the gene expression of RelA/p65, IGF-1, GLUT2, and SGLT1 in comparison with control. In addition, jejunal exposure to L-glutamine and oregano oil increased mRNA levels of GLUT2 compared with the control treatment. Exposure to oregano oil increased intestinal SGLT1 gene expression, while benzoic acid reduced SGLT1 expression compared with the control. Feed additives can differently modulate the gene expression of immune response, gut development, and glucose absorption in jejunal explants. These findings can contribute for a better understanding of the trophic action of these feed additives into the diets to optimize animal performance.
DOI: 10.6084/m9.figshare.7420235.v1
title: Roles of Hedgehog signaling pathway in adipogenic differentiation potential of porcine adipose-derived mesenchymal stem cells

description: ABSTRACT This study examined the effects of purmorphamine and cyclopamine, classical agonists and inhibitors of the Hedgehog (Hh) signaling pathway, in the adipogenic differentiation of porcine adipose-derived mesenchymal stem cells (AMSC) to investigate the roles underlying adipogenic differentiation in AMSC. Porcine-derived AMSC were established, and the Hh signaling pathway was activated or inhibited by treatment with purmorphamine or cyclopamine. The adipogenic differentiation of the porcine AMSC was then analyzed by Oil Red O staining. The expression levels of Hh signaling pathway factors and adipogenic transcription factors were determined using quantitative real-time polymerase chain reaction and western blot analysis. We verified that the expression levels of positive regulators of the Hh pathway (Smo, Gli1, Gli2, and Gli3) decreased during adipogenesis, whereas those of negative regulators (Ptc1 and Ptc2) increased. Purmorphamine can inhibit the adipogenic differentiation of porcine AMSC in vitro culture. In addition, both the expression of the CCAAT/enhancerbindingprotein-α and that of the peroxisome proliferator-activated receptor-γ decreased in the presence of purmorphamine. By contrast, cyclopamine had no significant effect on the adipogenic differentiation of porcine AMSC. The Hh signaling pathway inhibits the adipogenic differentiation potential of porcine AMSC.
DOI: 10.6084/m9.figshare.7420226
title: Induction of puberty and synchronization of estrus in gilts with eCG and GnRH

description: ABSTRACT In this study, we investigated the effects of eCG and GnRH on the induction of puberty in prepubertal gilts and evaluated the pregnancy rate and litter size in these females. Thirty 140-day-old gilts with an average weight of 80 kg were randomly divided into two treatments: hormonal treatment (n = 15): 1000 IU of eCG and 50 μg of GnRH; and control group (n = 15): induction of estrus by male presence. Gilts were inseminated at the second estrus and pregnancy was confirmed by the non-return to estrus. At farrowing, litter size, average piglet weight, and number of stillborn and mummified piglets were recorded for analysis. The variable pregnancy rate was subjected to the Chi-square test (χ2), while variables age at first estrus, weight at insemination, and duration of first and second estrus, litter size, average weight, and average number of dead and mummified piglets were analyzed by the t test. Hormonal treatment induced puberty in 100% of the gilts in four days. There was a difference in age at first estrus and in weight at insemination. Animals in the control group had the first estrus later and weighed more than those under hormonal treatment (167 vs. 143 days, 117 vs. 102 kg, respectively). However, there was no difference between the two groups in the duration of first and second estrus (54 vs. 49 h, 45 vs. 36 h, respectively), in pregnancy rate (100 vs. 86%, respectively), litter size, average piglet weight, and number of dead and mummified piglets between the hormonal treatment and control animals (9.0 vs. 9.8 piglets, 1.3 vs. 1.3 kg, and 3.0 vs. 2.3 dead and mummified, respectively). The hormonal treatment is effective in inducing precocious puberty without impairing pregnancy rate and litter size as well as average weight of piglets.
DOI: 10.6084/m9.figshare.7420229
title: Candidate mutations used to aid the prediction of genetic merit for female reproductive traits in tropical beef cattle

description: ABSTRACT In this study, we aimed to provide a wet laboratory validation for a set of single nucleotide polymorphisms (SNP), which had been identified as candidate functional variants in silico. Genotyping for candidate SNP was performed in Brahman and Tropical Composite cattle. After quality control, 29 SNP were first investigated individually for their association with female reproductive traits and then used as a panel for genomic predictions. The reproductive traits studied were age at first corpus luteum (AGECL; days), post-partum anoestrus interval (PPAI; days), and a binary trait that described if the cow had ovulated before weaning the first calf or not (PW, 0-1). Single nucleotide polymorphisms in six genes (FOXA2, TRAF4, IRF2, IRF1, BPTF, and CPEB1) were found to be significantly associated with reproduction traits . The genomic prediction method used was BayesR, to accommodate the 29 new SNP and compare their performance with predictions based on 50K genotypes (Illumina SNP chip). When new SNP and PLAG1 mutation rs109231213 were included in the genomic predictions for female reproductive traits their accuracies improved. The best predictions were obtained by combining the new SNP and the 50K SNP using BayesR analysis, with a 4% improvement in accuracy. The proportion of the genetic variance explained by the new SNP together was 0.07 for AGECL, 0.03 for PPAI, and 0.02 for PW. It would be favourable to include these new SNP in future versions of bovine SNP chips to target selection for female reproductive traits. These new SNP are likely to improve genomic predictions for female reproductive traits in tropical beef cattle breeds, with varying degrees of Bos indicus content.
DOI: 10.6084/m9.figshare.7420229.v1
title: Candidate mutations used to aid the prediction of genetic merit for female reproductive traits in tropical beef cattle

description: ABSTRACT In this study, we aimed to provide a wet laboratory validation for a set of single nucleotide polymorphisms (SNP), which had been identified as candidate functional variants in silico. Genotyping for candidate SNP was performed in Brahman and Tropical Composite cattle. After quality control, 29 SNP were first investigated individually for their association with female reproductive traits and then used as a panel for genomic predictions. The reproductive traits studied were age at first corpus luteum (AGECL; days), post-partum anoestrus interval (PPAI; days), and a binary trait that described if the cow had ovulated before weaning the first calf or not (PW, 0-1). Single nucleotide polymorphisms in six genes (FOXA2, TRAF4, IRF2, IRF1, BPTF, and CPEB1) were found to be significantly associated with reproduction traits . The genomic prediction method used was BayesR, to accommodate the 29 new SNP and compare their performance with predictions based on 50K genotypes (Illumina SNP chip). When new SNP and PLAG1 mutation rs109231213 were included in the genomic predictions for female reproductive traits their accuracies improved. The best predictions were obtained by combining the new SNP and the 50K SNP using BayesR analysis, with a 4% improvement in accuracy. The proportion of the genetic variance explained by the new SNP together was 0.07 for AGECL, 0.03 for PPAI, and 0.02 for PW. It would be favourable to include these new SNP in future versions of bovine SNP chips to target selection for female reproductive traits. These new SNP are likely to improve genomic predictions for female reproductive traits in tropical beef cattle breeds, with varying degrees of Bos indicus content.
DOI: 10.7284/116374
title: GNSS (Global Navigation Satellite System) data collected during research cruise NH1413 using a Furuno GP-90D instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7420226.v1
title: Induction of puberty and synchronization of estrus in gilts with eCG and GnRH

description: ABSTRACT In this study, we investigated the effects of eCG and GnRH on the induction of puberty in prepubertal gilts and evaluated the pregnancy rate and litter size in these females. Thirty 140-day-old gilts with an average weight of 80 kg were randomly divided into two treatments: hormonal treatment (n = 15): 1000 IU of eCG and 50 μg of GnRH; and control group (n = 15): induction of estrus by male presence. Gilts were inseminated at the second estrus and pregnancy was confirmed by the non-return to estrus. At farrowing, litter size, average piglet weight, and number of stillborn and mummified piglets were recorded for analysis. The variable pregnancy rate was subjected to the Chi-square test (χ2), while variables age at first estrus, weight at insemination, and duration of first and second estrus, litter size, average weight, and average number of dead and mummified piglets were analyzed by the t test. Hormonal treatment induced puberty in 100% of the gilts in four days. There was a difference in age at first estrus and in weight at insemination. Animals in the control group had the first estrus later and weighed more than those under hormonal treatment (167 vs. 143 days, 117 vs. 102 kg, respectively). However, there was no difference between the two groups in the duration of first and second estrus (54 vs. 49 h, 45 vs. 36 h, respectively), in pregnancy rate (100 vs. 86%, respectively), litter size, average piglet weight, and number of dead and mummified piglets between the hormonal treatment and control animals (9.0 vs. 9.8 piglets, 1.3 vs. 1.3 kg, and 3.0 vs. 2.3 dead and mummified, respectively). The hormonal treatment is effective in inducing precocious puberty without impairing pregnancy rate and litter size as well as average weight of piglets.
DOI: 10.6084/m9.figshare.7420232.v1
title: Feed additives can differentially modulate NF-κB (RelA/p65), IGF-1, GLUT2, and SGLT1 gene expression in porcine jejunal explants

description: ABSTRACT The intestinal gene expression of RelA/p65 (NF-κB), insulin growth factor 1 (IGF-1), glucose transporter 2 (GLUT2), and Na+/dependent glucose transporter 1 (SGLT1) were evaluated in response to benzoic acid, yeast culture, L-glutamine, and oregano essential oil, using an ex vivo model. Six piglets weighing approximately 20 kg each were sacrificed, and their jejunum was collected and segmented into five 2-cm explants. Each explant was immersed in cell culture medium according to one of the following treatments: control (without additive), 0.5% benzoic acid, 1% yeast culture, 1% L-glutamine, and 0.015% oregano oil. Gene expression was evaluated using RT-PCR. Yeast culture up-regulated the gene expression of RelA/p65, IGF-1, GLUT2, and SGLT1 in comparison with control. In addition, jejunal exposure to L-glutamine and oregano oil increased mRNA levels of GLUT2 compared with the control treatment. Exposure to oregano oil increased intestinal SGLT1 gene expression, while benzoic acid reduced SGLT1 expression compared with the control. Feed additives can differently modulate the gene expression of immune response, gut development, and glucose absorption in jejunal explants. These findings can contribute for a better understanding of the trophic action of these feed additives into the diets to optimize animal performance.
DOI: 10.6084/m9.figshare.7420214
title: Demand and income distribution: an analysis of the Brazilian economic growth between 1993-2013

description: Abstract This work aims to empirically investigate Brazilian economic growth from 1993 through 2013. It is based on Naastepad's model, which determines whether the demand-led growth is wage-led or profit-led. Thus, in order to determine the demand regime of the period, we estimated the equations for investment, exports and aggregate propensity to save. Estimating the equations allowed us to calculate the multiplier effect and investment and export elasticity effect. The demand regime in the period was wage-led, mostly due to low participation of investments and exportations in the national income. All equations were estimated by Ordinary Least Squares and showed robust standard deviation of parameters. The statistical tests, carried out after the estimation, suggest an unbiased and consistent model.
DOI: 10.7284/116371
title: Gyrocompass data collected during research cruise NH1413 using a Sperry MK-37 instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7420214.v1
title: Demand and income distribution: an analysis of the Brazilian economic growth between 1993-2013

description: Abstract This work aims to empirically investigate Brazilian economic growth from 1993 through 2013. It is based on Naastepad's model, which determines whether the demand-led growth is wage-led or profit-led. Thus, in order to determine the demand regime of the period, we estimated the equations for investment, exports and aggregate propensity to save. Estimating the equations allowed us to calculate the multiplier effect and investment and export elasticity effect. The demand regime in the period was wage-led, mostly due to low participation of investments and exportations in the national income. All equations were estimated by Ordinary Least Squares and showed robust standard deviation of parameters. The statistical tests, carried out after the estimation, suggest an unbiased and consistent model.
DOI: 10.6084/m9.figshare.7420205
title: Cardiovascular Manifestations of Erdheim-Chester's Disease: A Case Series

description: Abstract Erdheim-Chester Disease is a rare entity, classified as an inflammatory myeloid neoplasm, with an unknown incidence, occurring preferentially in men after 50 years of age. Classically, it has a multisystemic presentation, with the skeletal system being the most frequently affected (90% of the patients), followed by genitourinary involvement in 60% of cases and central nervous system in the pituitary and diabetes insipidus in 25% of the cases. Cardiovascular manifestations are present in more than half of the patients, with aortic infiltration and atrial pseudotumor being the most common forms.
DOI: 10.6084/m9.figshare.7420211
title: Pesquisa e Desenvolvimento na Cadeia Produtiva de Frangos de Corte no Brasil

description: Abstract: Over the last years, the production of Brazilian broiler increased by 112%, pushing up exports sharply. This excellent performance has settled Brazil as the largest exporter and third largest producer of broiler. Thousands of producers and hundreds of processing and exporting companies represent this sector. Its organization, use of technology and management skills have been an example of success for other meat chains. This success resulted from technological innovations, as well as the use of an efficient system of Research and Development (R&D). This article aims to analyze the R&D process in the production chain of Brazilian broiler, especially the stages of production and industrialization. A search had been carried out with the use of semi-structured questionnaires with professionals in the production and industrialization areas. The results showed that most of the surveyed companies has its own structure of R&D and develop research more strongly directed to the development of products or processes, some more intensively, looking for technological innovations, and others just test products already developed. A positive fact is that public institutions, such as universities and PRIs (Public Research Institutes), are important partners in this process, which demonstrates confidence in these institutions, as well as a concern to maximize physical, human and financial resources.
DOI: 10.7284/116368
title: Meteorological Station data collected during research cruise NH1413 using a SIO MET-System instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7420205.v1
title: Cardiovascular Manifestations of Erdheim-Chester's Disease: A Case Series

description: Abstract Erdheim-Chester Disease is a rare entity, classified as an inflammatory myeloid neoplasm, with an unknown incidence, occurring preferentially in men after 50 years of age. Classically, it has a multisystemic presentation, with the skeletal system being the most frequently affected (90% of the patients), followed by genitourinary involvement in 60% of cases and central nervous system in the pituitary and diabetes insipidus in 25% of the cases. Cardiovascular manifestations are present in more than half of the patients, with aortic infiltration and atrial pseudotumor being the most common forms.
DOI: 10.6084/m9.figshare.7420211.v1
title: Pesquisa e Desenvolvimento na Cadeia Produtiva de Frangos de Corte no Brasil

description: Abstract: Over the last years, the production of Brazilian broiler increased by 112%, pushing up exports sharply. This excellent performance has settled Brazil as the largest exporter and third largest producer of broiler. Thousands of producers and hundreds of processing and exporting companies represent this sector. Its organization, use of technology and management skills have been an example of success for other meat chains. This success resulted from technological innovations, as well as the use of an efficient system of Research and Development (R&D). This article aims to analyze the R&D process in the production chain of Brazilian broiler, especially the stages of production and industrialization. A search had been carried out with the use of semi-structured questionnaires with professionals in the production and industrialization areas. The results showed that most of the surveyed companies has its own structure of R&D and develop research more strongly directed to the development of products or processes, some more intensively, looking for technological innovations, and others just test products already developed. A positive fact is that public institutions, such as universities and PRIs (Public Research Institutes), are important partners in this process, which demonstrates confidence in these institutions, as well as a concern to maximize physical, human and financial resources.
DOI: 10.6084/m9.figshare.7420196
title: Influence of sediment distribution on the relationships among reservoir yield, spill, and evaporation losses

description: ABSTRACT This study shows how the sedimentation process in reservoirs affects the yield-spill-evaporation losses in reservoirs of Ceará State, Brazilian Northeast. Reservoirs are assumed to have, initially, inverted conical shape. Three forms of sedimentation were investigated: type 1, with deposition occurring parallel to the wetted perimeter; type 2, deposition distributed proportionally to the water depth; and, type 3, deposition concentrated in the reservoir bottom. These sedimentation patterns were found in many reservoirs in Ceará, with capacity ranging from about 0.5 to 100 hm3. Nevertheless, type 2 pattern was the most frequent. In this paper, five large reservoirs, over 100 hm3, were studied using Monte Carlo approach, and considering the silting over the time horizon. It was found that sediment distribution can significantly affect the yield-spill-evaporation trade-off on large reservoirs. Type 1 results have the lowest impact on reservoir yield, followed by type 2 and type 3. For Cedro reservoir, the yield would go to zero in 2115, assuming a type 3 deposition pattern. These results reinforce the need for monitoring sedimentation in large reservoirs in the Brazilian semiarid region. In addition, this study provides a relatively simple methodology to predict the impact of siltation on reservoir yield-spill-evaporation relationships, for the three most found patterns of sedimentation.
DOI: 10.6084/m9.figshare.7420202
title: Effect of auditory-motor training on auditory processing of school children

description: ABSTRACT Objective To compare performance in Avaliação Simplificada do Processamento Auditivo Central and Scale of Auditory Behaviors scores before and after auditory and motor training. Methods Sample comprising 162 children aged 9 to 11 years and attending public schools in the city of São Paulo (SP), Brazil; 122 out of 162 children were allocated to one of three experimental groups: Multisensory; Auditory/Motor and Motor/Auditory. Experimental groups were submitted to 8 hours of auditory, visuospatial and motor stimulation over the course of 8 weeks. The remaining 40 children formed the Control Group and received no stimulation. Results Relation between child behavior as perceived by school teachers and auditory test responses revealed that the better the performance in auditory processing assessment, the higher the Scale of Auditory Behaviors scores. Conclusion Auditory and motor training led to improvements in auditory processing skills as rated by Avaliação Simplificada do Processamento Auditivo Central and Scale of Auditory Behaviors; this intervention model proved to be a good tool for use in school settings.
DOI: 10.7284/116375
title: Meteorological Station data collected during research cruise NH1413 using a SIO MET-System instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7420202.v1
title: Effect of auditory-motor training on auditory processing of school children

description: ABSTRACT Objective To compare performance in Avaliação Simplificada do Processamento Auditivo Central and Scale of Auditory Behaviors scores before and after auditory and motor training. Methods Sample comprising 162 children aged 9 to 11 years and attending public schools in the city of São Paulo (SP), Brazil; 122 out of 162 children were allocated to one of three experimental groups: Multisensory; Auditory/Motor and Motor/Auditory. Experimental groups were submitted to 8 hours of auditory, visuospatial and motor stimulation over the course of 8 weeks. The remaining 40 children formed the Control Group and received no stimulation. Results Relation between child behavior as perceived by school teachers and auditory test responses revealed that the better the performance in auditory processing assessment, the higher the Scale of Auditory Behaviors scores. Conclusion Auditory and motor training led to improvements in auditory processing skills as rated by Avaliação Simplificada do Processamento Auditivo Central and Scale of Auditory Behaviors; this intervention model proved to be a good tool for use in school settings.