DOI: 10.5061/dryad.6k8b1
title: Data from: Occurrence and phylogenetic analysis of bovine respiratory syncytial virus in outbreaks of respiratory disease in Norway

description: Background: Bovine respiratory syncytial virus (BRSV) is one of the major
 pathogens involved in the bovine respiratory disease (BRD) complex. The
 seroprevalence to BRSV in Norwegian cattle herds is high, but its role in
 epidemics of respiratory disease is unclear. The aims of the study were to
 investigate the etiological role of BRSV and other respiratory viruses in
 epidemics of BRD and to perform phylogenetic analysis of Norwegian BRSV
 strains. Results: BRSV infection was detected either serologically and/or
 virologically in 18 (86%) of 21 outbreaks and in most cases as a single
 viral agent. When serology indicated that bovine coronavirus and/or bovine
 parainfluenza virus 3 were present, the number of BRSV positive animals in
 the herd was always higher, supporting the view of BRSV as the main
 pathogen. Sequencing of the G gene of BRSV positive samples showed that
 the current circulating Norwegian BRSVs belong to genetic subgroup II,
 along with other North European isolates. One isolate from an outbreak in
 Norway in 1976 was also investigated. This strain formed a separate branch
 in subgroup II, clearly different from the current Scandinavian sequences.
 The currently circulating BRSV could be divided into two different strains
 that were present in the same geographical area at the same time. The
 sequence variations between the two strains were in an antigenic important
 part of the G protein. Conclusion: The results demonstrated that BRSV is
 the most important etiological agent of epidemics of BRD in Norway and
 that it often acts as the only viral agent. The phylogenetic analysis of
 the Norwegian strains of BRSV and several previously published isolates
 supported the theory of geographical and temporal clustering of BRSV.
description: Phylogeny of the BRSV G
 genePhylogeny showing the
 relationship among 31 selected nucleotide sequences based on a 472 nt part
 from nucleotide number 178 to 649 of the G gene of BRSV. The sequences
 from this study, labelled with “*”, originated from 10 different farms
 with outbreaks of respiratory disease in Norway between 2009 and 2011, and
 one isolate from an outbreak in 1976. Included are also representatives of
 the six genetic subgroups of BRSV and recent published Swedish and Danish
 strains. The genetic subgroups (I-VI) and the strain type of the Norwegian
 sequences (A or B) are indicated on the right. The strains are indicated
 by the isolate name with GenBank Accession numbers AF188585, AF188586,
 AF188588, AF188604, JN619431, JN619439, JN619446, L27802, M58307, Y08717,
 Y08718, U92098, U92102, U92103, U24714-U24716, U33539 and U57823. The
 GenBank accession numbers of the Norwegian strains are: KF501149-KF501155,
 KF501159, KF501160 and KF501170-KF501172. The bootstrap values are shown
 next to the branches. The trees are drawn to scale, with branch lengths
 measured in the number of substitutions per site.Amino acid sequences 60
 to 215 of the G protein gene of BRSV isolatesComparison of deduced
 amino acid sequences. Designations on the left indicate the isolate ID.
 The GenBank accession number of the Norwegian strains are:
 KF501149-KF501155, KF501159, KF501160 and KF501170-KF501172 and for the
 Lelystad strain the number is U33539