cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.17702557.v1
title: Effect of a feed additive containing yeast cell walls, clove and coriander essential oils and <i>Hibiscus sabdariffa</i> administered to mid-lactating dairy cows on productive performance, rumen fluid composition and metabolic conditions

description: Thirty-six multiparous Holstein mid-lactating cows were housed in 6 pens (6 cows/pen) and allocated in two groups (3 pens/group), receiving a control total mixed ration (CTR), or the same diet supplemented with a feed additive (TRT). Between 15 and 35 days from enrolment (DFE) the dry matter intake (DMI) of each pen, and the individual milk yield (MY), rumination time (RT), the compositions of milk and rumen fluid and the metabolic profile of plasma were monitored regularly. At 35 DFE, cows started a 1-week wash-up period prior to the changeover. Data were analysed with a mixed model using repeated measures. Cows receiving the additive had higher DMI and MY (<i>p</i> &lt; .01), a longer RT between 22 and 29 DFE (<i>p</i> &lt; .05), a higher total VFA concentration and a lower pH in the rumen fluid (<i>p</i> &lt; .01 and <i>p</i> = .04, respectively). At 15 DFE, TRT cows had a higher concentration of lactose in their milk, and lower concentrations of BHB, ceruloplasmin and haptoglobin, paired with higher concentrations of albumin, paraoxonase and aspartate aminotransferase in their plasma (<i>p</i> &lt; .01). These outcomes suggest a positive effect of the additive on increasing DMI and MY, possibly reflecting that it had a combined action on the rumen and the liver.HighlightsThe additive increased feed intake, rumination time and milk yield.It improved rumen fermentations and liver activity, and mitigated ketogenesis and inflammatory conditions.Beneficial actions of the active components on rumen and liver functions could be hypothesised. The additive increased feed intake, rumination time and milk yield. It improved rumen fermentations and liver activity, and mitigated ketogenesis and inflammatory conditions. Beneficial actions of the active components on rumen and liver functions could be hypothesised.
DOI: 10.6084/m9.figshare.19329646
title: Influence of increasing levels of oregano essential oil on intestinal morphology, intestinal flora and performance of Sewa sheep

description: The purpose of this experiment was to study the effects of oregano essential oil (OEO) on the performance, intestinal morphology and intestinal flora of fattening Sewa sheep. Sixty 20-month-old Sewa sheep with similar body weights (BWs) and in good health were randomly divided into four groups. The groups were fed a diet containing 0 mg/kg OEO (group A), 150 mg/kg (group B), 300 mg/kg (group C) or 450 mg/kg (group D). Daily gain, slaughter rate and other production performance parameters were analysed in the test sheep (mean ± standard error). Intestinal morphology was analysed by tissue sectioning, and intestinal flora was analysed by 16S rRNA genome sequencing. The indexes, including average daily gain (ADG), slaughter rate, villus length of the small intestine and number of beneficial bacteria in the intestinal flora, in group C were significantly higher than those in the other groups. Supplementation with 300 mg/kg OEO concentrate in the diets can improve the growth performance of Sewa sheep by changing their intestinal morphology and modifying their intestinal flora structure. Conclusively, these encourage to further verifying in practice positive evidence in improving growth performance of OEO supplementation in the diets for Sewa sheep.HighlightsOregano essential oil (OEO) can improve the performance of Sewa sheep by changing the intestinal morphology and regulating the intestinal flora structure. Oregano essential oil (OEO) can improve the performance of Sewa sheep by changing the intestinal morphology and regulating the intestinal flora structure.
DOI: 10.6084/m9.figshare.21333024.v1
title: Advantages of label free method in comparison with 2DE proteomic analysis of <i>Butyrivibrio fibrisolvens</i> 3071 grown on different carbon sources

description: The aim of this study was the comparison of label-free method with 2DE to other analytical method of bacterium <i>Butyrivibrio fibrisolvens</i> extracellular protein samples. Label-free quantification (LF) method performed in this work was compared with previously obtained results of proteomic analysis using two-dimensional electrophoresis (2DE) and nano-liquid chromatography coupled with mass spectroscopy (nLC/MS). <i>B. fibrisolvens</i> as an important plant fibre degrader was cultivated on four different carbon sources (including xylan, xylose, glucose, and a mixture of xylan with glucose). The impact of growth substrate on protein profile was assessed by six pair-wise comparisons evaluating the significantly differently abundant extracellular proteins. Gel-free and gel-based methods resulted in substantially dissimilar results. The LC-MS/MS approach detected substrate-dependent differences in transport and binding membrane proteins (TBP) and nucleotidase, while the 2DE approach detected substrate effect on proteins included in protein synthesis and butyrate synthesis. On the other hand, both methods observed differentially regulated proteins involved in the glycolytic pathway, however, the only shared enzyme (protein detected both by 2DE and LC-MS/MS approach) was fructose-bisphosphate aldolase. The LC-MS/MS approach detected a high abundance of separated peptides. However, it cannot be easily considered as supreme to 2DE analysis. Both methods differ in sample preparation, their advantages and limitations. The study findings indicate these methods can complement each other and together they elucidate better the metabolic functions of <i>B. fibrisolvens</i>.HIGHLIGHTSComparison of LF and 2DE analysis showed limitations connected with a narrow optimised pH interval and number of affected proteins in 2DE in contrary to LF results.<i>Butyrivibrio fibrisolvens</i> culture showed the same proteomic changes in the presence of different saccharides as the whole rumen fluid in vivo.Selected proteins could be used for the monitoring of polysaccharides fermentation in the rumen and be used for optimisation hemicellulose degradation and overall feed utilisation. Comparison of LF and 2DE analysis showed limitations connected with a narrow optimised pH interval and number of affected proteins in 2DE in contrary to LF results. <i>Butyrivibrio fibrisolvens</i> culture showed the same proteomic changes in the presence of different saccharides as the whole rumen fluid in vivo. Selected proteins could be used for the monitoring of polysaccharides fermentation in the rumen and be used for optimisation hemicellulose degradation and overall feed utilisation.
DOI: 10.6084/m9.figshare.20448376
title: Haematological profiles of pigs of different age in relation to the presence or absence of porcine reproductive and respiratory virus, porcine circovirus type 2 and hepatitis E virus

description: This study was aimed to assessing haematological parameters in pigs of different age categories from six farms differing in the presence or absence of selected pathogens. The following categories were included: 5 age groups of growers, fatteners and breeding sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken. Group samples of oral fluid and faeces were collected from each farm and tested for detection of Porcine Reproductive and Respiratory Virus (PRRSV), Porcine Circovirus Type 2 (PCV2), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. On farms free of PRRSV, PCV2 and HEV, the age of pigs had significant effect on: white blood cell count (WBC), haemoglobin concentration (Hb), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration, platelet count (PLT), percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and ‘large unstained cells’ (LUCs). On positive farms all observed blood parameters except the percentage of LUCs were significantly affected by the age. The percentages of lymphocytes, mean corpuscular volume (MCV) and haematocrit were significantly lowered by PRRSV, while WBC, PLT, percentage and absolute numbers of neutrophils, basophils and LUCs were increased. Nine-week-old pigs with PCV2 had significantly lower red blood cell count, PLT, percentage and absolute numbers of monocytes and LUCs, and significantly higher MCV, MCH, percentage of neutrophils and percentage and absolute number of eosinophils than 9-week-old pigs with PCV2 and HEV. Age-related changes in haematological parameters occurred in PCR negative and positive farms.HIGHLIGHTSAge-related changes in haematological parameters were present in PCR negative farms and in PCR positive farms in relation to selected pathogens.Values for several haematological parameters in 9-week-old pigs with a monoinfection differed significantly from those in pigs of the same age with two viral infections.To interpret results of haematological analyses correctly, it is important to consider the haematological analyser used, the pig age and, health history, and clinical data. Age-related changes in haematological parameters were present in PCR negative farms and in PCR positive farms in relation to selected pathogens. Values for several haematological parameters in 9-week-old pigs with a monoinfection differed significantly from those in pigs of the same age with two viral infections. To interpret results of haematological analyses correctly, it is important to consider the haematological analyser used, the pig age and, health history, and clinical data.
DOI: 10.6084/m9.figshare.21333024
title: Advantages of label free method in comparison with 2DE proteomic analysis of <i>Butyrivibrio fibrisolvens</i> 3071 grown on different carbon sources

description: The aim of this study was the comparison of label-free method with 2DE to other analytical method of bacterium <i>Butyrivibrio fibrisolvens</i> extracellular protein samples. Label-free quantification (LF) method performed in this work was compared with previously obtained results of proteomic analysis using two-dimensional electrophoresis (2DE) and nano-liquid chromatography coupled with mass spectroscopy (nLC/MS). <i>B. fibrisolvens</i> as an important plant fibre degrader was cultivated on four different carbon sources (including xylan, xylose, glucose, and a mixture of xylan with glucose). The impact of growth substrate on protein profile was assessed by six pair-wise comparisons evaluating the significantly differently abundant extracellular proteins. Gel-free and gel-based methods resulted in substantially dissimilar results. The LC-MS/MS approach detected substrate-dependent differences in transport and binding membrane proteins (TBP) and nucleotidase, while the 2DE approach detected substrate effect on proteins included in protein synthesis and butyrate synthesis. On the other hand, both methods observed differentially regulated proteins involved in the glycolytic pathway, however, the only shared enzyme (protein detected both by 2DE and LC-MS/MS approach) was fructose-bisphosphate aldolase. The LC-MS/MS approach detected a high abundance of separated peptides. However, it cannot be easily considered as supreme to 2DE analysis. Both methods differ in sample preparation, their advantages and limitations. The study findings indicate these methods can complement each other and together they elucidate better the metabolic functions of <i>B. fibrisolvens</i>.HIGHLIGHTSComparison of LF and 2DE analysis showed limitations connected with a narrow optimised pH interval and number of affected proteins in 2DE in contrary to LF results.<i>Butyrivibrio fibrisolvens</i> culture showed the same proteomic changes in the presence of different saccharides as the whole rumen fluid in vivo.Selected proteins could be used for the monitoring of polysaccharides fermentation in the rumen and be used for optimisation hemicellulose degradation and overall feed utilisation. Comparison of LF and 2DE analysis showed limitations connected with a narrow optimised pH interval and number of affected proteins in 2DE in contrary to LF results. <i>Butyrivibrio fibrisolvens</i> culture showed the same proteomic changes in the presence of different saccharides as the whole rumen fluid in vivo. Selected proteins could be used for the monitoring of polysaccharides fermentation in the rumen and be used for optimisation hemicellulose degradation and overall feed utilisation.
DOI: 10.17876/plate/dr.3/envelopes/202_56600
title: Digital object identifier (DOI) for the envelope 56600 in archive 202 from the APPLAUSE DR3
DOI: 10.6084/m9.figshare.19329646.v2
title: Influence of increasing levels of oregano essential oil on intestinal morphology, intestinal flora and performance of Sewa sheep

description: The purpose of this experiment was to study the effects of oregano essential oil (OEO) on the performance, intestinal morphology and intestinal flora of fattening Sewa sheep. Sixty 20-month-old Sewa sheep with similar body weights (BWs) and in good health were randomly divided into four groups. The groups were fed a diet containing 0 mg/kg OEO (group A), 150 mg/kg (group B), 300 mg/kg (group C) or 450 mg/kg (group D). Daily gain, slaughter rate and other production performance parameters were analysed in the test sheep (mean ± standard error). Intestinal morphology was analysed by tissue sectioning, and intestinal flora was analysed by 16S rRNA genome sequencing. The indexes, including average daily gain (ADG), slaughter rate, villus length of the small intestine and number of beneficial bacteria in the intestinal flora, in group C were significantly higher than those in the other groups. Supplementation with 300 mg/kg OEO concentrate in the diets can improve the growth performance of Sewa sheep by changing their intestinal morphology and modifying their intestinal flora structure. Conclusively, these encourage to further verifying in practice positive evidence in improving growth performance of OEO supplementation in the diets for Sewa sheep.HighlightsOregano essential oil (OEO) can improve the performance of Sewa sheep by changing the intestinal morphology and regulating the intestinal flora structure. Oregano essential oil (OEO) can improve the performance of Sewa sheep by changing the intestinal morphology and regulating the intestinal flora structure.
DOI: 10.17876/plate/dr.3/envelopes/202_56601
title: Digital object identifier (DOI) for the envelope 56601 in archive 202 from the APPLAUSE DR3
DOI: 10.6084/m9.figshare.20448376.v1
title: Haematological profiles of pigs of different age in relation to the presence or absence of porcine reproductive and respiratory virus, porcine circovirus type 2 and hepatitis E virus

description: This study was aimed to assessing haematological parameters in pigs of different age categories from six farms differing in the presence or absence of selected pathogens. The following categories were included: 5 age groups of growers, fatteners and breeding sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken. Group samples of oral fluid and faeces were collected from each farm and tested for detection of Porcine Reproductive and Respiratory Virus (PRRSV), Porcine Circovirus Type 2 (PCV2), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. On farms free of PRRSV, PCV2 and HEV, the age of pigs had significant effect on: white blood cell count (WBC), haemoglobin concentration (Hb), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration, platelet count (PLT), percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and ‘large unstained cells’ (LUCs). On positive farms all observed blood parameters except the percentage of LUCs were significantly affected by the age. The percentages of lymphocytes, mean corpuscular volume (MCV) and haematocrit were significantly lowered by PRRSV, while WBC, PLT, percentage and absolute numbers of neutrophils, basophils and LUCs were increased. Nine-week-old pigs with PCV2 had significantly lower red blood cell count, PLT, percentage and absolute numbers of monocytes and LUCs, and significantly higher MCV, MCH, percentage of neutrophils and percentage and absolute number of eosinophils than 9-week-old pigs with PCV2 and HEV. Age-related changes in haematological parameters occurred in PCR negative and positive farms.HIGHLIGHTSAge-related changes in haematological parameters were present in PCR negative farms and in PCR positive farms in relation to selected pathogens.Values for several haematological parameters in 9-week-old pigs with a monoinfection differed significantly from those in pigs of the same age with two viral infections.To interpret results of haematological analyses correctly, it is important to consider the haematological analyser used, the pig age and, health history, and clinical data. Age-related changes in haematological parameters were present in PCR negative farms and in PCR positive farms in relation to selected pathogens. Values for several haematological parameters in 9-week-old pigs with a monoinfection differed significantly from those in pigs of the same age with two viral infections. To interpret results of haematological analyses correctly, it is important to consider the haematological analyser used, the pig age and, health history, and clinical data.
DOI: 10.17876/plate/dr.3/envelopes/202_56602
title: Digital object identifier (DOI) for the envelope 56602 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56603
title: Digital object identifier (DOI) for the envelope 56603 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56604
title: Digital object identifier (DOI) for the envelope 56604 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56605
title: Digital object identifier (DOI) for the envelope 56605 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56606
title: Digital object identifier (DOI) for the envelope 56606 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56607
title: Digital object identifier (DOI) for the envelope 56607 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56608
title: Digital object identifier (DOI) for the envelope 56608 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56609
title: Digital object identifier (DOI) for the envelope 56609 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56610
title: Digital object identifier (DOI) for the envelope 56610 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56611
title: Digital object identifier (DOI) for the envelope 56611 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56612
title: Digital object identifier (DOI) for the envelope 56612 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56613
title: Digital object identifier (DOI) for the envelope 56613 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56614
title: Digital object identifier (DOI) for the envelope 56614 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56615
title: Digital object identifier (DOI) for the envelope 56615 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56616
title: Digital object identifier (DOI) for the envelope 56616 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56617
title: Digital object identifier (DOI) for the envelope 56617 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56618
title: Digital object identifier (DOI) for the envelope 56618 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56619
title: Digital object identifier (DOI) for the envelope 56619 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56620
title: Digital object identifier (DOI) for the envelope 56620 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56621
title: Digital object identifier (DOI) for the envelope 56621 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56622
title: Digital object identifier (DOI) for the envelope 56622 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56623
title: Digital object identifier (DOI) for the envelope 56623 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56624
title: Digital object identifier (DOI) for the envelope 56624 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56625
title: Digital object identifier (DOI) for the envelope 56625 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56626
title: Digital object identifier (DOI) for the envelope 56626 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56627
title: Digital object identifier (DOI) for the envelope 56627 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56628
title: Digital object identifier (DOI) for the envelope 56628 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56629
title: Digital object identifier (DOI) for the envelope 56629 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56630
title: Digital object identifier (DOI) for the envelope 56630 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56631
title: Digital object identifier (DOI) for the envelope 56631 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56632
title: Digital object identifier (DOI) for the envelope 56632 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56633
title: Digital object identifier (DOI) for the envelope 56633 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56634
title: Digital object identifier (DOI) for the envelope 56634 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56635
title: Digital object identifier (DOI) for the envelope 56635 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56636
title: Digital object identifier (DOI) for the envelope 56636 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56637
title: Digital object identifier (DOI) for the envelope 56637 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56638
title: Digital object identifier (DOI) for the envelope 56638 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56639
title: Digital object identifier (DOI) for the envelope 56639 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56640
title: Digital object identifier (DOI) for the envelope 56640 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56641
title: Digital object identifier (DOI) for the envelope 56641 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56642
title: Digital object identifier (DOI) for the envelope 56642 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56643
title: Digital object identifier (DOI) for the envelope 56643 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56644
title: Digital object identifier (DOI) for the envelope 56644 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56645
title: Digital object identifier (DOI) for the envelope 56645 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56646
title: Digital object identifier (DOI) for the envelope 56646 in archive 202 from the APPLAUSE DR3
DOI: 10.17876/plate/dr.3/envelopes/202_56647
title: Digital object identifier (DOI) for the envelope 56647 in archive 202 from the APPLAUSE DR3