cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1597651.v2
title: Rhabditis sp. SB347 males attracted to unablated animals (wild type), and ablated animals for precursors of germline (Z2 & Z3), vulva precursor cells (VPC) and precursors of somatic gonad (Z1 & Z4)

description: Data from figure 3e. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. The values for the chemotaxis index (CI) were calculated with at least ten replicates for each condition. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction. Gonadal (Z1 – Z4) and vulva precursor cells (P5.p, P6.p and P7.p) of mid- L1 animals (females or hermaphrodites) were ablated using laser microsurgery. For ablation, worms were placed on agar pads on microscopic slides. Worms were recovered after ablation using a drop of M9 buffer and transferred to a normal seeded plate. Two days after recovery, laser ablated adult worms were used to collect supernatant for attraction assays
DOI: 10.6084/m9.figshare.1597650.v2
title: Attraction of Rhabditis sp. SB347 males to females in the fourth larval stage (L4) or as adults

description: Data from figure 3d. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. The values for the chemotaxis index (CI) shown in figures 3a, 3d, 3e (in x axis) and S1 were calculated with at least ten replicates for each condition. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction.
DOI: 10.6084/m9.figshare.1597651.v1
title: SB347 males attracted to unablated animals (wild type), and ablated animals for precursors of germline (Z2 & Z3), vulva precursor cells (VPC) and precursors of somatic gonad (Z1 & Z4)

description: Data from figure 3e. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. The values for the chemotaxis index (CI) were calculated with at least ten replicates for each condition. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction. Gonadal (Z1 – Z4) and vulva precursor cells (P5.p, P6.p and P7.p) of mid- L1 animals (females or hermaphrodites) were ablated using laser microsurgery. For ablation, worms were placed on agar pads on microscopic slides. Worms were recovered after ablation using a drop of M9 buffer and transferred to a normal seeded plate. Two days after recovery, laser ablated adult worms were used to collect supernatant for attraction assays
DOI: 10.6084/m9.figshare.1597653.v1
title: SB347 male attraction towards synthetic ascr#1 and ascr#9

description: Data from figure 4d. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction.
DOI: 10.6084/m9.figshare.1597649.v2
title: Percentage of Rhabditis sp. SB347 males that attempt to mate to females over time after mating

description: Data from figure 3c. The male response efficiency assay measures the percentage of males that attempt to mate with the opposite sex. One day before the experiment, NGM plates were spotted with 13 µL of E. coli OP50-1 culture. Before the experiment, 5 males were placed for 10 min in the center of the ~ 5 mm diameter bacterial spot to let them acclimatize. 15 animals of the opposite sex (hermaphrodites or females) were added to the bacterial spot and observed for 4 min. If a male attempted to mate, as determined by the male tail scanning the body of the other sex, the male response was scored with the number 1. In case there was no attempt, the response was scored as 0. The behavior of each male was recorded only once. For each type of mating (male/hermaphrodite or male/female), 5 replicates were performed. 25 mated females were divided in 5 sets. 15 males were added at 5 time points (0, 6, 12, 18 and 24 h) after the initial mating to each set.
DOI: 10.6084/m9.figshare.1597648
title: The percentage of Rhabditis sp. SB347 males that attempt to mate is higher when they are exposed to females than to hermaphrodites

description: Data from Figure 3b. The male response efficiency assay measures the percentage of males that attempt to mate with the opposite sex. One day before the experiment, NGM plates were spotted with 13 µL of E. coli OP50-1 culture. Before the experiment, 5 males were placed for 10 min in the center of the ~ 5 mm diameter bacterial spot to let them acclimatize. 15 animals of the opposite sex (hermaphrodites or females) were added to the bacterial spot and observed for 4 min. If a male attempted to mate, as determined by the male tail scanning the body of the other sex, the male response was scored with the number 1. In case there was no attempt, the response was scored as 0. The behavior of each male was recorded only once. For each type of mating (male/hermaphrodite or male/female), 5 replicates were performed.
DOI: 10.6084/m9.figshare.1597649.v1
title: Percentage of SB347 males that attempt to mate to females over time after mating

description: Data from figure 3c. The male response efficiency assay measures the percentage of males that attempt to mate with the opposite sex. One day before the experiment, NGM plates were spotted with 13 µL of E. coli OP50-1 culture. Before the experiment, 5 males were placed for 10 min in the center of the ~ 5 mm diameter bacterial spot to let them acclimatize. 15 animals of the opposite sex (hermaphrodites or females) were added to the bacterial spot and observed for 4 min. If a male attempted to mate, as determined by the male tail scanning the body of the other sex, the male response was scored with the number 1. In case there was no attempt, the response was scored as 0. The behavior of each male was recorded only once. For each type of mating (male/hermaphrodite or male/female), 5 replicates were performed. 25 mated females were divided in 5 sets. 15 males were added at 5 time points (0, 6, 12, 18 and 24 h) after the initial mating to each set.
DOI: 10.6084/m9.figshare.1597650.v1
title: Attraction of males to females in the fourth larval stage (L4) or as adults

description: Data from figure 3d. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. The values for the chemotaxis index (CI) shown in figures 3a, 3d, 3e (in x axis) and S1 were calculated with at least ten replicates for each condition. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction.
DOI: 10.6084/m9.figshare.1597653.v3
title: Rhabditis sp. SB347 male attraction towards synthetic ascr#1 and ascr#9

description: Data from figure 4d. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction.
DOI: 10.6084/m9.figshare.1597654.v1
title: SB347 sperm size

description: To release the sperm and measure their size, ten animals of each sex were cut using a 22 gauge needle in the presence of 5-10 µL drop of sperm buffer (90 mM NaCl, 50 mM KCl, 2 mM MgCl2, 10 mM CaCl2, 100 mM HEPES, and 20 mM dextrose to pH 7.8 for a 2X solution) 34. Males were cut at the level of the vas deferens and hermaphrodites at the level of the spermatheca 34. Immediately following the dissections, the slides were examined using DIC optics on a Carl Zeiss 510 confocal microscope. To improve the adhesion of the sperm cells to the slide, slides were pre-treated with Poly-L-Lysine. A 4-5 µL drop of 100% Poly-L-Lysine was placed at the center of the charged surface of a ColorFrost Plus slide. A second slide was placed on top of it in such a way that both charged surfaces were facing each other and a thin film of liquid formed between them. Once the film was formed, slides were separated by sliding them over each other. Subsequently, slides were baked at 60 °C for 1-2 h. To identify the sperm, which were often very small, we added Hoechst 33342 (Sigma-Aldrich) to the sperm media at a final concentration of 100 μg/mL. Hoechst helped to visualize and identify the characteristic condensed shape of the sperm nucleus. Sperm size was measured as the cross sectional area of the spermatid using the Carl Zeiss confocal software.
DOI: 10.6084/m9.figshare.1597647
title: Rhabditis sp. SB347 virgin females, mated females and hermaphrodites attraction to supernatant of males

description: Data from Figure 3a. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. The values for the chemotaxis index (CI) were calculated with at least ten replicates for each condition. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction. ‘Mated females’ were generated by placing single virgin females in contact with 4-5 males for 6 h.
DOI: 10.6084/m9.figshare.1597654.v3
title: Rhabditis sp. SB347 sperm size

description: To release the sperm and measure their size, ten animals of each sex were cut using a 22 gauge needle in the presence of 5-10 µL drop of sperm buffer (90 mM NaCl, 50 mM KCl, 2 mM MgCl2, 10 mM CaCl2, 100 mM HEPES, and 20 mM dextrose to pH 7.8 for a 2X solution). Males were cut at the level of the vas deferens and hermaphrodites at the level of the spermatheca. Immediately following the dissections, the slides were examined using DIC optics on a Carl Zeiss 510 confocal microscope. To improve the adhesion of the sperm cells to the slide, slides were pre-treated with Poly-L-Lysine. A 4-5 µL drop of 100% Poly-L-Lysine was placed at the center of the charged surface of a ColorFrost Plus slide. A second slide was placed on top of it in such a way that both charged surfaces were facing each other and a thin film of liquid formed between them. Once the film was formed, slides were separated by sliding them over each other. Subsequently, slides were baked at 60 °C for 1-2 h. To identify the sperm, which were often very small, we added Hoechst 33342 (Sigma-Aldrich) to the sperm media at a final concentration of 100 μg/mL. Hoechst helped to visualize and identify the characteristic condensed shape of the sperm nucleus. Sperm size was measured as the cross sectional area of the spermatid using the Carl Zeiss confocal software.
DOI: 10.6084/m9.figshare.1597646
title: Sexes of Rhabditis sp. SB347 F1 from selfing or outcrossing parents.

description: Data from Figure 2a. To determine the sex of the F1 from selfing hermaphrodites, the self-progeny of 20 SB347 animals was collected throughout their lifespan. The hermaphrodite mothers were selected by first isolating dauers, which in SB347 always develop into hermaphrodites. Dauers were placed into single 6 cm agar plates seeded with E. coli OP50-1 and left to mature into adults. Every 8 h of their reproductive life, the hermaphrodite mothers were transferred to a new plate until they stopped laying eggs. To control more precisely for worms that may escape the plate before being scored, we sexed the progeny while they were at larval stages (two days after hatching). We scored the three sexes using several traits, including developmental rates and morphologies. Dauer larvae (destined to become hermaphrodites) are thin with darkly-pigmented guts. Larger larvae with long thin tails develop into females. Male larvae have a characteristically blunt tail.
DOI: 10.6084/m9.figshare.1597648.v2
title: The percentage of Rhabditis sp. SB347 males that attempt to mate is higher when they are exposed to females than to hermaphrodites

description: Data from Figure 3b. The male response efficiency assay measures the percentage of males that attempt to mate with the opposite sex. One day before the experiment, NGM plates were spotted with 13 µL of E. coli OP50-1 culture. Before the experiment, 5 males were placed for 10 min in the center of the ~ 5 mm diameter bacterial spot to let them acclimatize. 15 animals of the opposite sex (hermaphrodites or females) were added to the bacterial spot and observed for 4 min. If a male attempted to mate, as determined by the male tail scanning the body of the other sex, the male response was scored with the number 1. In case there was no attempt, the response was scored as 0. The behavior of each male was recorded only once. For each type of mating (male/hermaphrodite or male/female), 5 replicates were performed.
DOI: 10.6084/m9.figshare.1597653.v2
title: Rhabditis sp. SB347 male attraction towards synthetic ascr#1 and ascr#9

description: Data from figure 4d. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction.
DOI: 10.6084/m9.figshare.1597654.v2
title: Rhabditis sp. SB347 sperm size

description: To release the sperm and measure their size, ten animals of each sex were cut using a 22 gauge needle in the presence of 5-10 µL drop of sperm buffer (90 mM NaCl, 50 mM KCl, 2 mM MgCl2, 10 mM CaCl2, 100 mM HEPES, and 20 mM dextrose to pH 7.8 for a 2X solution) 34. Males were cut at the level of the vas deferens and hermaphrodites at the level of the spermatheca 34. Immediately following the dissections, the slides were examined using DIC optics on a Carl Zeiss 510 confocal microscope. To improve the adhesion of the sperm cells to the slide, slides were pre-treated with Poly-L-Lysine. A 4-5 µL drop of 100% Poly-L-Lysine was placed at the center of the charged surface of a ColorFrost Plus slide. A second slide was placed on top of it in such a way that both charged surfaces were facing each other and a thin film of liquid formed between them. Once the film was formed, slides were separated by sliding them over each other. Subsequently, slides were baked at 60 °C for 1-2 h. To identify the sperm, which were often very small, we added Hoechst 33342 (Sigma-Aldrich) to the sperm media at a final concentration of 100 μg/mL. Hoechst helped to visualize and identify the characteristic condensed shape of the sperm nucleus. Sperm size was measured as the cross sectional area of the spermatid using the Carl Zeiss confocal software.
DOI: 10.6084/m9.figshare.1597645
title: Elevational variation in body-temperature response to immune challenge in a lizard

description: _
DOI: 10.6084/m9.figshare.1597636
title: The Nordic Landscape of LIS Research: A New Approach for Co-Word Analysis of Reserch in Three Nordic Countries

description: This study used a co-word analysis to map the landscape of library and information science in three Nordic countries:Denmark,SwedenandFinland. The results indicated that there are some strong similarities between LIS research in the studied countries and that mainstream LIS research still has a strong foothold, but newer areas of research, such as Library 2.0 and digital libraries, are gaining increasing interest. Furthermore the study showed that the new approach of self-generated co-words can be a fruitful method in mapping current research but also that the collection of data may sometimes be a problem.
DOI: 10.6084/m9.figshare.1597636.v1
title: The Nordic Landscape of LIS Research: A New Approach for Co-Word Analysis of Reserch in Three Nordic Countries

description: This study used a co-word analysis to map the landscape of library and information science in three Nordic countries:Denmark,SwedenandFinland. The results indicated that there are some strong similarities between LIS research in the studied countries and that mainstream LIS research still has a strong foothold, but newer areas of research, such as Library 2.0 and digital libraries, are gaining increasing interest. Furthermore the study showed that the new approach of self-generated co-words can be a fruitful method in mapping current research but also that the collection of data may sometimes be a problem.
DOI: 10.6084/m9.figshare.1597647.v3
title: SB347 virgin females, but not mated females or hermaphrodites (H), are attracted to supernatant of males

description: Data from Figure 3a. To quantify the degree of attraction of worms to conditioned medium produced by different sexes, we used chemotaxis assays. Conditioned medium (henceforth referred to as ‘supernatant’) was generated by placing 5-10 worms in 100 µL of M9 buffer for 12 h at room temperature. The supernatant was stored in 10 µL aliquots at -20 °C until required. The assay was performed either on a microscope slide with a layer of 1.5% agar placed on top of an empty plate, or on a 6 cm NGM plate. 3 µL of supernatant was added on one side of the slide and 3 cm apart from the control drop that just contained M9. 15 young-adults were placed on the midline between the spots. After 30 min, worms were scored on the basis of their location. The values for the chemotaxis index (CI) were calculated with at least ten replicates for each condition. To calculate the CI, the number of worms attracted to the test spot were subtracted by the number of worms in the control spot, and divided by the total number of males assayed. A CI of one indicates that all worms are attracted to the chemical in the test spot, whereas a CI close to zero indicates no attraction. ‘Mated females’ were generated by placing single virgin females in contact with 4-5 males for 6 h.