cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/5z1vb-02g75
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C111
description: <strong>Tilt Series Date:</strong> 2012-02-18</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-10/3dimage_68745/</p>12feb18_C111_pNL_INF_AT1BT10011_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-10/3dimage_68745/12feb18_C111_pNL_INF_AT1BT10011_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb18_C111_pNL_INF_AT1BT10011.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-10/rawdata/12feb18_C111_pNL_INF_AT1BT10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb18_C111_pNL_INF_AT1BT10011_full.tiff,  file associated to 3D Image #68745, 814.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-10/file_15973/12feb18_C111_pNL_INF_AT1BT10011_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/97v14-0hj25
title: COS7 VPS4DN+CHMP4A

description: Cos7 cells co-transfected with VPS4DN and CHMP4A. Sample #C104
description: <strong>Tilt Series Date:</strong> 2012-02-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN and CHMP4A constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-7/3dimage_68692/</p>12feb04_ATBT1_0007_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-7/3dimage_68692/12feb04_ATBT1_0007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0007.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-7/rawdata/12feb04_ATBT1_0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0007_full.tiff,  file associated to 3D Image #68692, 1.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-7/file_15951/12feb04_ATBT1_0007_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8c21s-jct58
title: Myxococcus xanthus LL061

description: mCherry-bacP.First loaded in Titan cartridge and imaged in cryoLM.Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 29100x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-7/rawdata/</p>LL061_UCLA_correlated_20130424_17.mrc,  Tilt Series (Pixel Size 1.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-7/rawdata/LL061_UCLA_correlated_20130424_17.mrc"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_17_full.rec,  Reconstruction (Pixel Size 2.0 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-7/3dimage_26279/LL061_UCLA_correlated_20130424_17_full.rec"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_17_bin2_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 314.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-7/3dimage_26781/LL061_UCLA_correlated_20130424_17_bin2_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rgkpe-c2p03
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-4/3dimage_68945/</p>12jan14_C107_1_pNL_DN_AT1BT10001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-4/3dimage_68945/12jan14_C107_1_pNL_DN_AT1BT10001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT1BT10001.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-4/rawdata/12jan14_C107_1_pNL_DN_AT1BT10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT1BT10001_full.tiff,  file associated to 3D Image #68945, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-4/file_16057/12jan14_C107_1_pNL_DN_AT1BT10001_full.tiff"
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DOI: 10.22002/bwn3w-7zj56
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-4/rawdata/</p>tomography4middle.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-4/rawdata/tomography4middle.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography4middle.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-4/3dimage_47354/tomography4middle.rec"
        > <i class="download icon"></i> Download </a></p>

        middle4.rec,  Reconstruction (Pixel Size 0.0 nm), 133.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-4/3dimage_47858/middle4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.7910/dvn/w0ofm6
title: U.S. Imports of Merchandise: Comprehensive United states Import data.

description: The United States may be said to have had an adequate set of import and export statistics only since 1821. During the years 1795-1801, import value figures were estimated by the Secretary of the Treasury and the figures for the years 1790-1794 and from 1802-1820 were apparently estimated many years later. In the case of exports, it was required by statute that the dollar value in the statistics should be the value reported by the master of the vessel on which the goods left the country, but it was common practice for the U.S. Collectors of Customs to adjust this reported value quite arbitrarily. By an Act approved February 1820, ``to provide for obtaining accurate statements of the Foreign Commerce of the United States,'' a provision was made for the preparation of statistical accounts of the commerce of the United States with foreign countries by the Register of the Treasury to show the kinds, quantities and values of all articles exported to and of all articles imported from each foreign country. These early and simple beginnings of foreign trade data have developed into the current comprehensive export and import statistical program. The statistics are used extensively by both the public and private sectors of the country.
DOI: 10.22002/bzb1v-had92
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-16</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-4/3dimage_68977/</p>12jan16_pNLDN_AT1BT10010_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-4/3dimage_68977/12jan16_pNLDN_AT1BT10010_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan16_pNLDN_AT1BT10010.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-4/rawdata/12jan16_pNLDN_AT1BT10010.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan16_pNLDN_AT1BT10010_full.tiff,  file associated to 3D Image #68977, 965.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-16-4/file_16074/12jan16_pNLDN_AT1BT10010_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mj5nf-jt040
title: COS7

description: Cos7 - not treatment, no transfection
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cells frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and plunge frozen</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-15/3dimage_69027/</p>12jan20_C106_2_COS7neg_AT1BT1_0026_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-15/3dimage_69027/12jan20_C106_2_COS7neg_AT1BT1_0026_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0026.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-15/rawdata/12jan20_C106_2_COS7neg_AT1BT1_0026.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0026_full.tiff,  file associated to 3D Image #69027, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-15/file_16096/12jan20_C106_2_COS7neg_AT1BT1_0026_full.tiff"
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DOI: 10.22002/01td4-n6d97
title: Myxococcus xanthus LL061

description: mCherry-bacP.First loaded in Titan cartridge and imaged in cryoLM.Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 29100x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-4/rawdata/</p>LL061_UCLA_correlated_20130424_21.mrc,  Tilt Series (Pixel Size 1.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-4/rawdata/LL061_UCLA_correlated_20130424_21.mrc"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_21_full.rec,  Reconstruction (Pixel Size 2.0 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-4/3dimage_26276/LL061_UCLA_correlated_20130424_21_full.rec"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_21_bin2_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 314.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-4/3dimage_26786/LL061_UCLA_correlated_20130424_21_bin2_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zwyqc-89095
title: COS7

description: Cos7 - not treatment, no transfection
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cells frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and plunge frozen</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-8/3dimage_69013/</p>12jan20_C106_2_COS7neg_AT1BT1_0014_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-8/3dimage_69013/12jan20_C106_2_COS7neg_AT1BT1_0014_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0014.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-8/rawdata/12jan20_C106_2_COS7neg_AT1BT1_0014.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0014_full.tiff,  file associated to 3D Image #69013, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-8/file_16090/12jan20_C106_2_COS7neg_AT1BT1_0014_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jeaw0-pk846
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-9/3dimage_68865/</p>12jan06_c105_1_4ADNCos7_AT2BT1_0007_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
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        12jan06_c105_1_4ADNCos7_AT2BT1_0007.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        12jan06_c105_1_4ADNCos7_AT2BT1_0007_full.tiff,  file associated to 3D Image #68865, 878.9 kB  
        
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