DOI: 10.5061/dryad.q58hr
title: Data from: The genetics of mate preferences in hybrids between two young and sympatric Lake Victoria cichlid species

description: The genetic architecture of mate preferences is likely to affect
 significant evolutionary processes, including speciation and
 hybridization. Here, we investigate laboratory hybrids between a pair of
 sympatric Lake Victoria cichlid fish species that appear to have recently
 evolved from a hybrid population between similar predecessor species. The
 species demonstrate strong assortative mating in the laboratory,
 associated with divergent male breeding coloration (red dorsum versus
 blue). We show in a common garden experiment, using DNA-based paternity
 testing, that the strong female mate preferences among males of the two
 species are fully recovered in a large fraction of their F2 hybrid
 generation. Individual hybrid females often demonstrated consistent
 preferences in multiple mate choice trials (more than or equal to five)
 across a year or more. This result suggests that female mate preference is
 influenced by relatively few major genes or genomic regions. These
 preferences were not changed by experience of a successful spawning event
 with a male of the non-preferred species in a no-choice single-male trial.
 We found no evidence for imprinting in the F2 hybrids, although the F1
 hybrid females may have been imprinted on their mothers. We discuss this
 nearly Mendelian inheritance of consistent innate mate preferences in the
 context of speciation theory.
description: Table S1. F2 Spawning
 decisions of F2 hybrid femalesTable S1 contains a
 summary of the spawning decisions of all F2 hybrid females, including
 their IDs, brood numbers and fathers. 1-32 is the experimental round and
 each round may include more than one spawning decision per female. 0 =
 spawning decision with P. sp. "pundamilia-like" 1 = spawning
 decision with P. sp. "nyererei-like" out = female isolated in a
 tank with a male of the non-preferred species in = female re-introduced
 into the experiment out = female isolated in a tank with a male of the
 non-preferred species in = female re-inroduced into the
 experimentSvensson_et_al_Table_S1_CVS.csvTable S2. Spawning decisions of wild type females and F1 femalesTable S2 contains a summary of the spawning decisions of all wild type females and F1 hybrid females 1-32 is the experimental round and each round may include more than one spawning decision per female. Species: p = P. sp. "pundamilia like" n = P. sp. "nyererei-like" pn = F1 hybrid between female P. sp. "pundamilia like" and male P. sp. "nyererei-like" np = F1 hybrid between female P. sp. "nyererei-like" and male P. sp. "pundamilia like" 0 = spawning decision with P. sp. "pundamilia-like" 1 = spawning decision with P. sp. "nyererei-like"Svensson_et_al_Table_S2_CVS.csvTable S3. Males used in the spawning experiment: Microsatellite genotypes and experimental roundMales used in the spawning experiment. ID is the identification code. IDs starting with m were lab bred males. All others males were wild caught. Spec is the species. 1&2-30-32 are the experimental rounds. The numbers are the experimental rounds and the positions of the compartments. 11 is the first round and the first compartment, 12 is the first round and the second compartment etc. 21 is the second round and the first compartment, 22 is the second round and the second compartment etc. Ppun7-Ppun32 are the microsatellite genotypes of the males (in bp)Svensson_et_al_Table_S3_CVS.csvTable S4. Raw data and paternal analysesTable S4 includes microsatellite raw data of the offspring of the F2 females (q1-35 and 1-286), the wild type females (p and n) and the F1 hybrid females (pn and np), as well as the paternity analyses. ID (Blank): The ID of the female used in Table S1-2, Brood No: The numer of the brood, Prefix: The prefix in the PIT-tag number, Suffix: The suffix in the PIT-tag number, SL (mm): Standard length (mm), TL (mm): Total lenght (mm), Weight (g), No fry: Number of fry in the brood, Date stripped, No broods, Experimental round: Experimental round in Table S1-2, (Blank), Mother: Result of maternal analyses (same as ID if left blank), Father: Result of the paternity analyses, Ppun7a: Microsatellite lengt in base pairs (bp), Ppun7b: Microsatellite lengt in base pairs (bp), Ppun5a: Microsatellite lengt in base pairs (bp), Ppun5b: Microsatellite lengt in base pairs (bp), Ppun21a: Microsatellite lengt in base pairs (bp), Ppun21b: Microsatellite lengt in base pairs (bp), Ppun17a: Microsatellite lengt in base pairs (bp), Ppun17b: Microsatellite lengt in base pairs (bp), Ppun32a: Microsatellite lengt in base pairs (bp), Ppun32b: Microsatellite lengt in base pairs (bp).Svensson_et_al_Table_S4.xlsx