cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11266820.v1
title: E-learning in Pharmacy Education: what do we know about it?

description: The increase and flexibilization of e-learning in Brazil has generated concern about the low capacity to ensure the quality of institutions and courses. The objective is to identify the literature on experiences of pharmaceutical e-learning. A scope review was carried out, mapping key concepts and evidence. The selected articles, without period restriction, from the Scielo, Pubmed and Scopus databases provided data on the educational level of experience, modality, workload, content delivery method, instruments used, types of topics, evaluation information and purpose of the study. There were 87 articles on preparation for subsequent classes; feedback or continuation of previous classes; to specific training; the comparison between face-to-face and distance education modalities; and distance learning. Among the subjects treated, 51.8% involved pharmaceutical clinic and 42.5% basic science. Positive student assessment was found in more than 92% of cases. Isolation, and deficits in social and analytical skills were negative points. Technology infrastructure can impact learning. In Brazil, the health area has manifested opposition to the use of distance education in undergraduate studies. There is no evidence on the organization of undergraduate distance courses, but there are elements indicating risks to the results of training and development of professional skills in this modality.
DOI: 10.6084/m9.figshare.11997633
title: The improvement of anti-HER2 scFv soluble expression in Escherichia coli

description: The relationship between the expression of HER2 and malignity of breast tumors has led to the generation of antibodies targeting HER2+ tumors. In addition, the expression of scFvs, as the smallest antigen-binding region of antibody containing two disulfide bonds in Escherichia coli often results in accumulating non-functional protein in the cytoplasm. A redox-modified strain of E. coli such as Origami (DE3) may facilitate the formation of proper disulfide bond in cytoplasm. The present study aimed to optimize the expression of anti-HER2 scFv in Origami and evaluate the influence of induction temperature, and host strain on the solubility of the protein. To this aim, chemicallysynthesized anti-HER2 scFv of Trastuzumab was cloned in pET-22b (+). The results demonstrated that anti-HER2 scFv is expressed in Origami, purified by using Ni-NTA column, and detected by anti-His antibody in Western blot analysis. The highest anti-HER2 scFv expression in Origami was achieved 24 h after IPTG induction (1 mM) at 37 ºC. Further, the total anti-HER2 scFv expression level was higher in BL21, compared to Origami strain. However, the ratio of soluble/insoluble forms of anti-HER2 scFv increased in Origami strain. Furthermore, higher soluble expression was achieved when the culture of recombinant Origami was conducted at lower temperature (25 ºC).
DOI: 10.6084/m9.figshare.11997633.v1
title: The improvement of anti-HER2 scFv soluble expression in Escherichia coli

description: The relationship between the expression of HER2 and malignity of breast tumors has led to the generation of antibodies targeting HER2+ tumors. In addition, the expression of scFvs, as the smallest antigen-binding region of antibody containing two disulfide bonds in Escherichia coli often results in accumulating non-functional protein in the cytoplasm. A redox-modified strain of E. coli such as Origami (DE3) may facilitate the formation of proper disulfide bond in cytoplasm. The present study aimed to optimize the expression of anti-HER2 scFv in Origami and evaluate the influence of induction temperature, and host strain on the solubility of the protein. To this aim, chemicallysynthesized anti-HER2 scFv of Trastuzumab was cloned in pET-22b (+). The results demonstrated that anti-HER2 scFv is expressed in Origami, purified by using Ni-NTA column, and detected by anti-His antibody in Western blot analysis. The highest anti-HER2 scFv expression in Origami was achieved 24 h after IPTG induction (1 mM) at 37 ºC. Further, the total anti-HER2 scFv expression level was higher in BL21, compared to Origami strain. However, the ratio of soluble/insoluble forms of anti-HER2 scFv increased in Origami strain. Furthermore, higher soluble expression was achieved when the culture of recombinant Origami was conducted at lower temperature (25 ºC).
DOI: 10.6084/m9.figshare.11266820
title: E-learning in Pharmacy Education: what do we know about it?

description: The increase and flexibilization of e-learning in Brazil has generated concern about the low capacity to ensure the quality of institutions and courses. The objective is to identify the literature on experiences of pharmaceutical e-learning. A scope review was carried out, mapping key concepts and evidence. The selected articles, without period restriction, from the Scielo, Pubmed and Scopus databases provided data on the educational level of experience, modality, workload, content delivery method, instruments used, types of topics, evaluation information and purpose of the study. There were 87 articles on preparation for subsequent classes; feedback or continuation of previous classes; to specific training; the comparison between face-to-face and distance education modalities; and distance learning. Among the subjects treated, 51.8% involved pharmaceutical clinic and 42.5% basic science. Positive student assessment was found in more than 92% of cases. Isolation, and deficits in social and analytical skills were negative points. Technology infrastructure can impact learning. In Brazil, the health area has manifested opposition to the use of distance education in undergraduate studies. There is no evidence on the organization of undergraduate distance courses, but there are elements indicating risks to the results of training and development of professional skills in this modality.
DOI: 10.6084/m9.figshare.11452965
title: In vitro biological activities of silver nanoparticles synthesized from Scedosporium sp. isolated from soil

description: One of the important fields in nanotechnology is the development of an environment friendly method for the synthesis of nanoparticles. Many approaches show that microorganisms are the most reliable tools for biosynthesis of nanoparticles compared to physical and chemical methods. In our study, fungi have been exploited for extracellular production of metal nanoparticles. It was observed that in Scedosporium, silver ions are reduced to silver nanoparticles, which was confirmed by UV-visible spectrophotometry and AFM. Optimization studies showed that as the concentration of AgNO3 used for synthesis increased, particles’ size also increased. Size of the particles at different concentrations of AgNO3 was observed to be 79-107 nm with particles being ellipsoidal to spherical in shape. Silver nanoparticles synthesized from 2.0 mM silver nitrate, showed maximum antimicrobial activity compared to all antibiotics tested including synergistic effects. In vitro cytotoxicity of silver nanoparticles against MCF 7 and PC 3 showed that as the concentration of silver nanoparticles increased, a decrease in the percentage cell viability was observed with IC50 values being 60.09 and 57.43 µg/ml respectively. Therefore, through this study, it could be said that extracellular synthesis of silver nanoparticles from Scedosporium was simple, ecofriendly, proving excellent antimicrobial and anticancer agents.
DOI: 10.6084/m9.figshare.11452965.v1
title: In vitro biological activities of silver nanoparticles synthesized from Scedosporium sp. isolated from soil

description: One of the important fields in nanotechnology is the development of an environment friendly method for the synthesis of nanoparticles. Many approaches show that microorganisms are the most reliable tools for biosynthesis of nanoparticles compared to physical and chemical methods. In our study, fungi have been exploited for extracellular production of metal nanoparticles. It was observed that in Scedosporium, silver ions are reduced to silver nanoparticles, which was confirmed by UV-visible spectrophotometry and AFM. Optimization studies showed that as the concentration of AgNO3 used for synthesis increased, particles’ size also increased. Size of the particles at different concentrations of AgNO3 was observed to be 79-107 nm with particles being ellipsoidal to spherical in shape. Silver nanoparticles synthesized from 2.0 mM silver nitrate, showed maximum antimicrobial activity compared to all antibiotics tested including synergistic effects. In vitro cytotoxicity of silver nanoparticles against MCF 7 and PC 3 showed that as the concentration of silver nanoparticles increased, a decrease in the percentage cell viability was observed with IC50 values being 60.09 and 57.43 µg/ml respectively. Therefore, through this study, it could be said that extracellular synthesis of silver nanoparticles from Scedosporium was simple, ecofriendly, proving excellent antimicrobial and anticancer agents.
DOI: 10.6084/m9.figshare.11997711
title: Bovine serum albumin-based nanoparticles containing the flavonoid rutin produced by nano spray drying

description: Rutin is a flavonoid compound obtained from different vegetables and fruits; specifically, it is found in the seeds of buckwheat and in fruit peels, particularly citrus. It is also an important constituent of red wine. Rutin exhibits various biological properties including antiviral, vasoprotective, anti-inflammatory, and anticarcinogenic activities. However, its antioxidant activity is the most well studied. Despite the potential for in vitro applications, rutin presents low oral bioavailability that affects its biological activities. Nanoparticles composed of polymers, protein, or lipids are of great importance in the pharmaceutical and nutraceutical areas due to their physicochemical properties, which improve the pharmacokinetics of the drug which is loaded within. This study presents the production of bovine serum albumin (BSA) nanoparticles containing rutin by nano spray drying. Nanoparticles were characterized in terms of mean particle size, size distribution, morphology, zeta potential, and drug content; as well as their antioxidant activity. The optimized spray-drying conditions produced spherical particles with a mean size of 316 nm, zeta potential of −32 mV, and encapsulation efficiency around 32%. Moreover, when antioxidant activity toward the ABTS+ radical was assayed, nanoencapsulation increased the IC50 of rutin by 2-fold. The nano spray-drying process proved to be suitable for the production of rutin-loaded BSA nanoparticles with potential antioxidant activity.
DOI: 10.6084/m9.figshare.11997711.v1
title: Bovine serum albumin-based nanoparticles containing the flavonoid rutin produced by nano spray drying

description: Rutin is a flavonoid compound obtained from different vegetables and fruits; specifically, it is found in the seeds of buckwheat and in fruit peels, particularly citrus. It is also an important constituent of red wine. Rutin exhibits various biological properties including antiviral, vasoprotective, anti-inflammatory, and anticarcinogenic activities. However, its antioxidant activity is the most well studied. Despite the potential for in vitro applications, rutin presents low oral bioavailability that affects its biological activities. Nanoparticles composed of polymers, protein, or lipids are of great importance in the pharmaceutical and nutraceutical areas due to their physicochemical properties, which improve the pharmacokinetics of the drug which is loaded within. This study presents the production of bovine serum albumin (BSA) nanoparticles containing rutin by nano spray drying. Nanoparticles were characterized in terms of mean particle size, size distribution, morphology, zeta potential, and drug content; as well as their antioxidant activity. The optimized spray-drying conditions produced spherical particles with a mean size of 316 nm, zeta potential of −32 mV, and encapsulation efficiency around 32%. Moreover, when antioxidant activity toward the ABTS+ radical was assayed, nanoencapsulation increased the IC50 of rutin by 2-fold. The nano spray-drying process proved to be suitable for the production of rutin-loaded BSA nanoparticles with potential antioxidant activity.
DOI: 10.6084/m9.figshare.14307069.v1
title: Accumulation dynamics and physicochemical variability of starch in cultivars of Canna edulis Ker

description: ABSTRACT Canna edulis, also known as achira or sagu, is widely grown in South America because its rhizomes are a major source of starch for food and agroindustry. This study aimed to assess the accumulation rate and physicochemical changes of starch from four canna cultivars (Verde, Nativa, Maituna and Morada), grown under a traditional system, in the southwest region of Colombia. The rhizomes were harvested after planting (between five and nine months) to extract and characterize the starch. It was found that the starch yield (% wb) was related to the plant development age, reaching a maximum at eight months for all cultivars (12.78 ± 0.19 % - Verde; 12.46 ± 0.18 % - Maituna; 12.17 ± 0.19 % - Nativa; 11.10 ± 0.18 % - Morada). The average chemical composition (% wb) of the native starch throughout the rhizome development, for all cultivars, was: 86.68 % of starch, 1.12 % of protein, 0.43 % of dietary fiber, 0.14 % of ash and 11.57 % of moisture. At the optimum harvest age, the minimum and maximum amylose contents were 45.63 % (Maituna) and 54.47 % (Verde). The starch granule size among the cultivars showed a normal distribution, with a range of 40 µm to 80 µm and mean of 59.9 µm. The pasting curves per cultivar showed that the starch generated very high viscosity gels, unstable to the heating-cooling cycles, and high retrogradation.
DOI: 10.6084/m9.figshare.14307068.v1
title: Initial production and quality of camu-camu fruits under organic and mineral fertilization

description: ABSTRACT Camu-camu [Myrciaria dubia (Kunth) McVaugh] is a native plant of the Amazon basin floodplains that is facing a domestication process in dry land. This study aimed to evaluate, qualitatively and quantitatively, camu-camu fruits obtained at the first harvest, in dry land areas, under mineral and organic fertilization. The experimental design was randomized blocks, with 10 treatments and 4 replications. Each experimental plot consisted of 4 lines with 5 holes (3 plants hole-1). Fertilization with organic residues (filter cake and compost + filter cake) every four months provided a higher fruit yield (326 g plant-1). However, the yield was lower when mineral fertilization or its combination with organic matter (76 ± 40 g plant-1) was used. Fertilization with filter cake + compost increased the fruit weight (8.91 g) and size (2.47 cm in diameter and 2.30 cm in length). The mineral and organic fertilization did not influence the chemical characteristics of the camu-camu fruits.
DOI: 10.6084/m9.figshare.14307065.v1
title: Glyphosate-induced impact on the functional traits of the Bacillus sp. FC1 isolate

description: ABSTRACT The intensive use of glyphosate has shown to be harmful to non-target organisms. Considering the soil as a final herbicide acceptor, the microbial community present on it is one of the critical factors to be monitored. This study aimed to isolate Bacillus sp., as well as to evaluate the effect of glyphosate on functional traits related to the growth and biocontrol activity of the phytopathogen. Bacillus sp. FC1 was isolated from the soil and grown in six media, in the presence and absence of glyphosate. The analysis of bacterial growth consisted of comparing the growth curves. The effect of glyphosate on the biocontrol activity was evaluated by antibiosis against the phytopathogen Fusarium sp. Glyphosate had a negative effect on the growth of the Bacillus sp. FC1 isolate. Exposure to the herbicide, based on the antibiosis method, showed no effect on the biocontrol activity of the phytopathogen. However, the sensitivity of the isolate to the herbicide may have affected its ability to initially compete for nutrients with the phytopathogen in the medium.
DOI: 10.6084/m9.figshare.14307062.v1
title: Screening of cowpea accessions for cowpea aphid resistance

description: ABSTRACT Cowpea (Vigna unguiculata) genotypes may preserve important characteristics of potential use for the genetic breeding of this species. This study aimed to characterize sources of genetic resistance to the cowpea aphid (Aphis craccivora), among accessions maintained at the cowpea germplasm bank of the Universidade Federal do Ceará (Brazil), in order to suggest crossing options for obtaining superior cultivars. Twenty-one local accessions were assessed together with two genotypes as resistant checks and two others as susceptible checks. A randomized complete block design, with four replications, was used. Counts of live adults and nymphs per plant were used to evaluate the genotypic resistance and, based on the ranking of these data, the effective resistance was estimated. The applied approach showed to be effective to discriminate the genetic variability for cowpea aphid resistance, identifying antibiosis as the probable resistance mechanism. Four genotypic groups were identified: resistant, intermediate, susceptible and highly susceptible. Thus, for a short-time cultivar release strategy, backcrosses are suggested between the BRS Guariba cultivar (recurrent parental, with good agronomic traits) and aphid-resistant accessions, such as Enrica Pobre (CE-36), Das Almas (CE-07), CE-51 (selected within CE-13) and Ritinha (CE-08). However, for a long-term breeding strategy, crossings between the most dissimilar accessions are suggested, using resistant genitors (e.g. BRS Guariba, Enrica Pobre, Das Almas, CE-51 and Ritinha) and others with good commercial and agronomic attributes.
DOI: 10.5281/zenodo.4636739
title: data set related to articole Exploring the Efficacy of a Mindfulness Program for Boys With Attention-Deficit Hyperactivity Disorder and Oppositional Defiant Disorder

description: This record contains raw data related to articole to Exploring the Efficacy of a Mindfulness Program for Boys With Attention-Deficit Hyperactivity Disorder and Oppositional Defiant Disorder
DOI: 10.5281/zenodo.4636738
title: data set related to articole Exploring the Efficacy of a Mindfulness Program for Boys With Attention-Deficit Hyperactivity Disorder and Oppositional Defiant Disorder

description: This record contains raw data related to articole to Exploring the Efficacy of a Mindfulness Program for Boys With Attention-Deficit Hyperactivity Disorder and Oppositional Defiant Disorder
DOI: 10.6084/m9.figshare.14307060.v1
title: Selection of fungi with biocontrol potential against the black spot disease of papaya1

description: ABSTRACT The use of fungicides is the main control measure against the black-spot disease of papaya. The biological control is an alternative to that, being the selection of mycoparasitic fungi the first step in programs aiming at this kind of control. This study aimed to obtain and select fungi with potential to the biocontrol of the black spot disease of papaya. For this purpose, 24 isolates were collected from different regions and pathosystems, and then identified morphologically and by the ITS region sequencing. In order to evaluate the mycoparasite potential, two assessments, in a randomized block design, with three replications, were carried out in a shadehouse, being the obtained isolates inoculated on papaya leaves with black spot lesions. The average time for the appearance of mycoparasitism signs and the incidence of mycoparasitized black spot lesions were evaluated. Of the 24 isolates obtained, ten were from Hansfordia pulvinata, two from Lecanicillium lecaniium, two from Simplicillium lanossoniveum, one from Sarocladium implicatum and nine from Acremonium spp. A wide variability, concerning the mycoparasitism on black spot lesions, was observed, especially for the isolates H-611, H-613, H-614 and H-615, which showed the highest colonization averages. The results demonstrate that H. pulvinata has a great potential to be used as a biocontrol agent against Asperisporium caricae.
DOI: 10.6084/m9.figshare.14307059.v1
title: Growth and contents of organic and inorganic solutes in amaranth under salt stress

description: ABSTRACT Amaranthus cruentus L. is a forage species, with grains that exhibit excellent nutritional characteristics, being the 'BRS Alegria' the first cultivar recommended for cultivation in Brazil. This study aimed at evaluating the effect of salt stress on the growth and concentrations of organic and inorganic solutes in Amaranthus cruentus L. ('BRS Alegria' cultivar). Height, stem diameter, number of leaves, leaf, stem and root dry mass, leaf area, relative water content and membrane integrity percentage, as well as soluble carbohydrate, free amino acid, soluble protein, free proline, Na+, Cl- and K+ contents, were evaluated in different plant organs. Salinity significantly reduced the biomass yield and leaf area from the treatment with 25 mM of NaCl, indicating that the 'BRS Alegria' cultivar is sensitive to salt stress. Soluble carbohydrates in the leaves decreased by 59 %, while the other organic solutes showed no substantial increases. These results, coupled with the reduction in the relative water content and membrane integrity, suggest a low ability of this cultivar to adjust osmotically under salt stress. The K+/Na+ ratio abruptly decreased in 25 mM of NaCl, suggesting an ionic imbalance, which may partially explain the salt-induced growth reduction.
DOI: 10.6084/m9.figshare.14307057.v1
title: Portable chlorophyll meter can estimate the nitrogen sufficiency index and levels of topdressing nitrogen in common bean

description: ABSTRACT The nitrogen sufficiency index (NSI) obtained by using a portable chlorophyll meter is a practical alternative to evaluate the N levels in plants. This study aimed at determining the appropriate NSI value for leaves of common bean plants, in order to define the need and amount of N to be applied as topdressing, using the Minolta SPAD-502 portable chlorophyll meter. Three field experiments were conducted using a randomized blocks design, with four replications. Nitrogen doses were applied as topdressing and compared with a treatment in which a high dose of N was used as a reference to calculate the NSI, whose value was 95 %. According to the results observed using the chlorophyll meter and the NSI, for each 0.1 percentage point increase in the NSI to reach the adequate level (95 %), it is necessary to apply 1.1-1.5 kg ha-1 of topdressing N.