cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/x697x-q0169
title: Clostridium thermocellum

description: cellulosome
description: <strong>Tilt Series Date:</strong> 2013-07-29</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Clostridium thermocellum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Matson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth on cellulobiose, anaerobic</p>
  etl2013-07-29-5_slicer7958.jpg: Clostridium thermocellum .  peptidoglycan etl2013-07-29-5_slicer19945.jpg: Cellulosome?  .  95nm etl2013-07-29-5_slicer19946.jpg: Cellulosome?  .  117nm etl2013-07-29-5_slicer19993.jpg: cellulosome? 112 nm - Nicholas etl2013-07-29-5_slicer19994.jpg: What is this? 280 nm- Nicholas etl2013-07-29-5_slicer19995.jpg: What is this? 280 nm- Nicholas
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-29-5/3dimage_29388/</p>cthermo0005_full.rec,  Reconstruction (Pixel Size 2.4 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-29-5/3dimage_29388/cthermo0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        cthermo0005.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-29-5/rawdata/cthermo0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        etl2013-07-29-5_slicer19282.png,  657.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/etl2013-07-29-5_slicer19282.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2gjem-zyx63
title: Myxococcus xanthus delta-pomXYZ

description: Grid box YWC123(sent to Janelia)
description: <strong>Tilt Series Date:</strong> 2015-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 10ml cells into 100ml fresh CTT at 30C shaking for 2 days. Resuspend cells with pipet to break up clumps. Spun down 100ml culture in SS-34 rotor for 10 minutes at 5,000rcf. Discard pellet. Spin supernatant for 15 minutes at 30,000rcf.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-01-39/rawdata/</p>YWC123-B-tomo0028.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-01-39/rawdata/YWC123-B-tomo0028.mrc"
        > <i class="download icon"></i> Download </a></p>

        YWC123-B-tomo0028_full.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-01-39/3dimage_55679/YWC123-B-tomo0028_full.rec"
        > <i class="download icon"></i> Download </a></p>

        ycw2015-05-01-39_slicer14037.png,  402.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2015-05-01-39_slicer14037.png"
        > <i class="download icon"></i> Download </a></p>

        ycw2015-05-01-39_slicer14039.png,  396.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2015-05-01-39_slicer14039.png"
        > <i class="download icon"></i> Download </a></p>

        ycw2015-05-01-39_slicer14043.png,  398.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2015-05-01-39_slicer14043.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pwmsw-jat33
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-10-22</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-98/rawdata/</p>Fjoh1_58.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-98/rawdata/Fjoh1_58.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q73c0-jra51
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-11-08</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-57.0°, 57.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-88/rawdata/</p>Fjoh4_55.mrc,  Tilt Series (Pixel Size 0.39 nm), 170.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-88/rawdata/Fjoh4_55.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/47h2g-pqx40
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2019-12-07</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-07-135/rawdata/</p>Cc_MMM_20191207.mrc,  Tilt Series (Pixel Size 0.353 nm), 8.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-07-135/rawdata/Cc_MMM_20191207.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.9swn2h
title: Occurrence Download

description: A dataset containing 238 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Eigenmannia limbata)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 238 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045067-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/wr6pv-z9842
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-12-21</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-12-21-1/rawdata/</p>AnchorHM.mrc,  Tilt Series (Pixel Size 0.53 nm), 60.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-12-21-1/rawdata/AnchorHM.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.kk9w84
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus viminalis",
 "Country is United Kingdom of Great Britain and Northern Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 6 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045069-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.xx9dce
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Montecincla jerdoni",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045068-250811113504898/datasets/export for details.
DOI: 10.22002/q4n8e-vyv72
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-10-22</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-59/rawdata/</p>Fjoh1_6.mrc,  Tilt Series (Pixel Size 0.39 nm), 427.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-59/rawdata/Fjoh1_6.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rnt5q-10c18
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-12-21</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-12-21-2/rawdata/</p>AnchorLM.mrc,  Tilt Series (Pixel Size 0.53 nm), 241.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-12-21-2/rawdata/AnchorLM.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9r684-qrh97
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-11-08</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-57.0°, 57.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-22/rawdata/</p>Fjoh3_35.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-22/rawdata/Fjoh3_35.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1g0jm-bwf26
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-10-22</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-89/rawdata/</p>Fjoh1_49.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-89/rawdata/Fjoh1_49.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jfggh-smb41
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2019-12-07</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-07-134/rawdata/</p>Cc_atlas_20191207.mrc,  Tilt Series (Pixel Size 0.353 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-07-134/rawdata/Cc_atlas_20191207.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kz9s3-bmj68
title: D. flos-aquae Gas Vesicles

description: Gas vesicles purified from D. flos-aquae, WT
description: <strong>Tilt Series Date:</strong> 2020-11-04</p>
<strong>Data Taken By:</strong> Przemek Dutka</p>
<strong>Species / Specimen:</strong> Dolichospermum flos-aquae</p>
<strong>Strain:</strong> CCAP 1403/13F</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: 2.5 μm,  magnification: 64000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> purified by Dina Malounda the Shapiro lab; data collected by Przemek . Dutka and Songye Chen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Lakshmanan, A., Lu, G., Farhadi, A. et al. Preparation of biogenic gas . vesicle nanostructures for use as contrast agents for ultrasound and . MRI. Nat Protoc 12, 2050–2080 (2017). . https://doi.org/10.1038/nprot.2017.081. Sample in 10mM Hepes, pH7.4; OD 5.0</p>
<strong>Sample Preparation:</strong> Quantifoil C2/2 200mesh extra thick carbon, 10nm BSA-gold, 100% . humidity, Blot time 4, Blot force 3, temp 4C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-3/rawdata/</p>AnaGVOD5_13_movies.mrc,  Tilt Series (Pixel Size 0.14 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-3/rawdata/AnaGVOD5_13_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        AnaGVOD5_13_movies_part41_10.rec,  Reconstruction (Pixel Size 0.56 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-3/3dimage_115860/AnaGVOD5_13_movies_part41_10.rec"
        > <i class="download icon"></i> Download </a></p>

        AnaGVOD5_13.mrc.mdoc,  26.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-3/rawdata/AnaGVOD5_13.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kbpfg-3q675
title: Ecoli_EPEC_8612

description: These are E coli cells (EPEC 8612) obtained from Ritesh (Ilan Rosenshine lab at the Hebrew  .  university). The idea is to image bacterial nanotubes in these cells.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These cells were prepared with Ritesh in Ilan Rosenshine lab at the Hebrew University. </p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. Then washed with PBS and blotted and then plunge . frozen. Yuxi helped in sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-35/rawdata/</p>EPEC_8612_tilt13.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-35/rawdata/EPEC_8612_tilt13.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt13_part35_6.rec,  Reconstruction (Pixel Size 1.796 nm), 501.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-35/3dimage_110694/EPEC_8612_tilt13_part35_6.rec"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt13.mrc.mdoc,  19.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-35/rawdata/EPEC_8612_tilt13.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rwek6-8nv29
title: BacillusSubtilis_328_4hr

description: This is Bacillus Subtilis tomograms PonA mutant strain 328 (obtained from Amit in Sigal Ben  .  Yehuda lab at the Hebrew university). The idea is to image bacterial nanotubes in Bacilus .  Subtilis.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB328, ΔponA ΔymdB amyE::ymdB Δhag</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates . (I will write the details on my Labjournal).  </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were grown on the grids on agar plates for four hours. Detailed description is in the lab . journal. Yuxi helped in the sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> Samples were taken from the agar plates and washed few times in PBS. Then they were blotted . and plunge-frozen.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-15/rawdata/</p>Bsubtilis_328_4hr_conventional7.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
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        Bsubtilis_328_4hr_conventional7_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
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        Bsubtilis_328_4hr_conventional7.mrc.mdoc,  23.2 kB  
        
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