cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/6wx-bs6-gyt
title: Mirabilis expansa (Ruiz & Pav.) Standl.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/nzf-faa-zy2
title: Petrorhagia graminea (Sm.) P. W. Ball & Heywood

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/9hw-r9q-2ty
title: Astragalus monspessulanus subsp. monspessulanus

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/wrb-41w-g26
title: Cerastium pedunculare Bory & Chaub.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/z66-xed-t4p
title: Allionia incarnata L.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/4fw-bar-5zy
title: Mirabilis prostrata (Ruiz & Pav.) Heimerl

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/4wq-jcx-hvd
title: Astragalus monspessulanus subsp. monspessulanus

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/ef9-gjq-8nm
title: Crucianella latifolia L.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/vrb-8z4-8b2
title: Filago eriocephala Guss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/6kv-2ze-4lk
title: Alkanna graeca Boiss. & Spruner

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/ll2-pjg-94n
title: Onosma erecta Sm.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/jn1-chr-20r
title: Bougainvillea modesta Heimerl

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/vag-anb-3h1
title: Centaurea pulchella Ledeb.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/g9r-9qy-ng1
title: Centaurea iberica Trevis. ex Spreng.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/r7p-wnk-h2t
title: Centaurea kilaea Boiss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/zp8-zg1-pjp
title: Centaurea kilaea Boiss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/2xq-h82-wyc
title: Centaurea kilaea Boiss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/gnn-783-fyw
title: Cyperus javanicus Houtt.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/43a-ntd-6bl
title: Mirabilis prostrata (Ruiz & Pav.) Heimerl

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/mse-0xb-h3a
title: Chingia christii (Copel.) Holttum

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/4m8-xkk-rel
title: Chingia christii (Copel.) Holttum

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/t6z-89a-tpe
title: Nyctaginaceae Juss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/cmx-a2l-c5e
title: Centaurea austroanatolica Hub.-Mor.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/g3k-aje-jtt
title: Centaurea virgata Lam.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/q0l-34c-4ty
title: Centaurea cariensis subsp. maculiceps (O. Schwarz) Wagenitz

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/d7h-922-rmq
title: Centaurea cariensis subsp. maculiceps (O. Schwarz) Wagenitz

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/lax-649-fvy
title: Centaurea cariensis subsp. maculiceps (O. Schwarz) Wagenitz

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/e6h-hbz-46a
title: Leucanthemum platylepis Borbás

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/vpp-cxc-mw0
title: Centaurea polyclada DC.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/8b0-t1e-ka5
title: Centaurea cariensis Boiss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/fkk-l3w-n27
title: Centaurea cariensis subsp. maculiceps (O. Schwarz) Wagenitz

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/j36-6cc-1kg
title: Leucanthemum platylepis Borbás

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/8k7-rhw-vax
title: Leucanthemum platylepis Borbás

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/yv9-45p-eqt
title: Leucanthemum ircutianum subsp. leucolepis (Briq. & Cavill.) Vogt & Greuter

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/d4z-32g-lv7
title: Centaurea cariensis Boiss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/e1s-3g5-wq2
title: Tephroseris palustris (L.) Fourr.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/h7l-d2k-3l9
title: Centaurea cariensis subsp. maculiceps (O. Schwarz) Wagenitz

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/hjx-hpn-06c
title: Mirabilis L.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/3pr-0sn-smv
title: Rubiaceae

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/ez3-bv4-z8m
title: Rubiaceae

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/whk-zfh-08h
title: Delphinium cruciatum M. Hossain & P. H. Davis

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/1xz-phk-t0z
title: Nyctaginaceae Juss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/v6l-fqg-xmv
title: Delphinium hellesponticum Boiss.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/8rs-0r2-q8p
title: Centaurea luschaniana Heimerl

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/fhp-a7r-64t
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/0xp-83a-9z4
title: Festuca chrysophylla Phil.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/ve4-fd8-a8w
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/2tg-5y8-n95
title: Festuca chrysophylla Phil.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/6fq-zal-5tb
title: Tephroseris integrifolia subsp. capitata (Wahlenb.) B. Nord.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/w3t-v23-d9y
title: Festuca chrysophylla Phil.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/8as-npd-j1n
title: Festuca Tourn. ex L.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/jsp-b2g-6r0
title: Tephroseris integrifolia subsp. caucasigena (Schischk.) Greuter

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/0ac-zz1-n1h
title: Tephroseris integrifolia subsp. caucasigena (Schischk.) Greuter

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/561-1k1-d8v
title: Tephroseris integrifolia subsp. atropurpurea (Ledeb.) B. Nord.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/l6k-yj5-77a
title: Limonium solandri (Webb & Berthel.) Kunkel

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/6f5-r08-73n
title: Limonium pectinatum (Aiton) Kuntze

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/yp7-00s-qvz
title: Centaurea virgata Lam.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/0j2-7dt-n8z
title: Tephroseris crispa (Jacq.) Rchb.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/ahc-gpw-dfy
title: Tephroseris (Rchb.) Rchb.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/9ww-w5m-31v
title: Centaurea luschaniana Heimerl

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/ged-e8c-pab
title: Tephroseris integrifolia subsp. integrifolia

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/4tq-cvb-8pm
title: Pisoniella arborescens (Lag. & Rodr.) Standl.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/dvp-sy5-6l3
title: Callisthene Mart.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/zas-7eg-37k
title: Barleria L.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/faw-1gr-zm1
title: Barleria irritans Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/jd0-ps6-fsf
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/0mg-0mf-6jg
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/8xs-hyk-r30
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/416-fwa-bwj
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/w78-4vl-b3v
title: Barleria rigida Willd. ex Nees

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.3535/zlb-0sq-tl5
title: Centaurea virgata Lam.

description: Digital Specimen for the physical specimen hosted at Botanic Garden and Botanical Museum Berlin.
DOI: 10.15468/dl.d63m7j
title: Occurrence Download

description: A dataset containing 94 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Sarasinula plebeia"
 ]
}

 The dataset includes 94 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000632-250710112747441/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j01g0kxq
title: CIL: 54453, Homo sapiens. In Cell Image Library

description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
description: Imaging mode: bright-field microscopy
Visualization methods: 4G8 IHC using HRP/DAB
description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled for amyloid-beta (4G8).
DOI: 10.6075/j06q1wxs
title: CIL: 54460, Homo sapiens. In Cell Image Library

description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled by Bielschowsky's silver stain.
description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
description: Imaging mode: bright-field microscopy
Visualization methods: silver nitrate and ammonium hydroxide
DOI: 10.6075/j0183678
title: CIL: 54485, Homo sapiens. In Cell Image Library

description: Imaging mode: bright-field microscopy
Visualization methods: silver nitrate and ammonium hydroxide
description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled by Bielschowsky's silver stain.
description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
DOI: 10.6075/j0ft8kxx
title: CIL: 54834, Simonsiella muelleri, bacteria. In Cell Image Library

description: Imaging mode: phase contrast microscopy
description: Nikon Eclipse NI-U microscope equipped with a MFCool camera (Jenoptik).
description: C. steedae filaments
DOI: 10.6075/j0vh5nkr
title: CIL: 54488, Homo sapiens. In Cell Image Library

description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
description: Imaging mode: bright-field microscopy
Visualization methods: 4G8 IHC using HRP/DAB
description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled for amyloid-beta (4G8).
DOI: 10.6075/j0ng4qgn
title: CIL: 54818, Alysiella filiformis, bacteria. In Cell Image Library

description: Nikon Eclipse NI-U microscope equipped with a MFCool camera (Jenoptik).
description: Imaging mode: fluorescence microscopy
Visualization methods: fluorescent d amino acids
description: A. filiformis filament after incubation with HADA for 30 minutes.
DOI: 10.6075/j0mp52xr
title: CIL: 54438, Homo sapiens. In Cell Image Library

description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human non-symptomatic donor labeled for amyloid-beta (4G8).
description: Imaging mode: bright-field microscopy
Visualization methods: 4G8 IHC using HRP/DAB
description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
DOI: 10.6075/j0bz65w6
title: CIL: 54641, Mus musculus, oocyte. In Cell Image Library

description: SI FIG. 1 Structure of actin layer fenestrae and relationship with plasma membrane.
description: Oocytes fixed with 2% paraformaldehyde and 1% picric acid. Immunolabelled with anti-Calreticulin (green), anti-CD9(white), Alexafluor 568-phalloidin (red), and Hoechst 33342 (blue).
description: Imaging mode: whole mount confocal image
Visualization methods: Laser Scan Confocal; PMT
DOI: 10.6075/j0qc0357
title: CIL: 54467, Homo sapiens. In Cell Image Library

description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
description: Imaging mode: bright-field microscopy
Visualization methods: hematoxylin and eosin
description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled for hematoxylin and eosin.
DOI: 10.6075/j0v40v1c
title: CIL: 54615, Phakopsora Pachyrhizi. In Cell Image Library

description: Imaging mode: time lapse microscopy
description: Processed snapshot images, with binarized single-fungus ROIs.
description: The sequenced reference P. pachyrhizi strain K8108 is a single urediniosoral isolate from P. pachyrhizi-infected soybean leaves collected in 2015 in Uruguay (Colonia region). Genome sequence of this isolate is available at 
https://mycocosm.jgi.doe.gov/PhapaK8108/PhapaK8108.home.html.
DOI: 10.6075/j0jq10nj
title: CIL: 54424, Homo sapiens. In Cell Image Library

description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human non-symptomatic donor labeled for phosphorylated tau (AT8).
description: Imaging mode: bright-field microscopy
Visualization methods: AT8 IHC using HRP/DAB
description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
DOI: 10.6075/j0kk9bnn
title: CIL: 54832, Alysiella filiformis, bacteria. In Cell Image Library

description: Imaging mode: fluorescence microscopy
Visualization methods: fluorescent d amino acids
description: S. muelleri filaments after incubation with BADA for 30 minutes.
description: Nikon Eclipse NI-U microscope equipped with a MFCool camera (Jenoptik).
DOI: 10.6075/j03j3cn3
title: CIL: 54468, Homo sapiens. In Cell Image Library

description: 5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled for amyloid-beta (4G8).
description: Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.
description: Imaging mode: bright-field microscopy
Visualization methods: 4G8 IHC using HRP/DAB
DOI: 10.6075/j0416wx5
title: CIL: 54727. In Cell Image Library

description: Item type: EM tomogram
Imaging mode: Electronic tomography
description: This image displays the PHF array.
description: We examined the structural organization of cytoskeletal components and membrane systems in neurons of well preserved biopsy material from Alzheimer’s disease (AD). Information was obtained from thin sectioned material using conventional electron microscopy and from thick sections with the high voltage electron microscope.
DOI: 10.6075/j00k28f6
title: CIL: 54665, Drosophila melanogaster, astrocytes (red). In Cell Image Library

description: Imaging mode: fluorescence microscopy
Visualization methods: Spinning Disk Confocal Imaging
description: Confocal image of the CNS of a D. melanogaster in the third instar larval stage after Spz3 KD, 40uM from the ventral surface, depicting astrocytes (red), cortex glia (green) and neuronal nuclei (blue). Our study examined the breakdown of the spatial segregation between cortex glia and astrocyte after disruption of cortex glial morphology. After knockdown of Spz3, a secreted neurotrophin, we observed aberrant infiltration by astrocytes into the cortex.
description: Astrocyte-specific RFP expression was achieved using driven using the Gal4-UAS binary system and the driver alrm. Cortex-glia specific GFP was achieved using the lexA-lexAop binary system and the cortex-glia specific driver wrapper932i-LexA (Coutinho-Budd et al., 2017). The larval CNS was dissected in the third instar larval stage, fixed with methanol, stained for RFP, GFP and Elav (neuronal nuclei marker), and mounted in VectaShield reagent. Slides were imaged on an Innovative Imaging Innovations (3i) spinning disk confocal microscope equipped with a Yokogawa CSX-W1 using a 63X oil objective.

Staining: Astrocytes (red); Cortex glia (green); Neurons (blue)