cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/ztw-1p7-d1n
title: Veronica persica Poir.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/0rz-sdk-7ft
title: Carex appendiculata (Trautv. & C.A.Mey.) Kük.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/kn2-p24-md3
title: Hedysarum micropterum Bunge ex Boiss.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/4r1-tyy-xn0
title: Matthiola odoratissima (M. Bieb.) W. T. Aiton

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/yke-z66-dfl
title: Macromitrium parvifolium Dixon

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.5281/zenodo.13951768
title: Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking

description: This upload contains raw data and simulation software underlying the results presented in a manuscript submitted for peer-review, with the title:

 

Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking

 

Authored by:

C Venugopal Srambickal1,*, H Esmaeeli1,*, J Piguet1, L Reinkensmeier2, R Siegmund2, A Agostinho3 ,M Bates2, A Egner2, J Widengren1,**

1 Experimental Biomolecular Physics, Bio-Opto-Nano Unit, Department of Applied Physics, Royal Institute of Technology, SE-10691 Stockholm, Sweden

2 Department of Optical Nanoscopy, Institute for Nanophotonics, D-37077 Göttingen, Germany

3 Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-17165 Solna, Sweden

* Contributed equally

** Corresponding author: jwideng@kth.se

 

ABSTRACT

MINimal photon FLUXes (MINFLUX) offers super-resolution microscopy (SRM) with nanometer localization precision, with lower fluorophore brightness and photostability requirements than for other SRM techniques. Nonetheless, low localization probabilities have been reported in several MINFLUX studies, and a broader use of less bright and photostable fluorophores, including near-infrared (NIR) fluorophores has been difficult to realize. In this work, we identified fluorophore blinking as a main cause of erroneous (and dismissed) fluorophore localizations in MINFLUX imaging and devised strategies to overcome these effects. We systematically studied the blinking/switching properties of cyanine fluorophores emitting in the far-red or NIR range, over typical time scales (µs-10ms), sample and excitation conditions used in MINFLUX imaging. Subsequent simulations of representative MINFLUX localization procedures showed that trans-cis isomerization, and in particular photo-reduction of the fluorophores, can generate significant localization errors. These localization errors, however, could be suppressed by balanced redox buffers and repetitive excitation beam scans. Implementing these strategies, and replacing the slower, intrinsic switching of the fluorophores needed for the localization by transient binding of fluorophore-labelled DNA strands to complementary DNA strands attached to the targets (DNA-PAINT), we could for the first time demonstrate NIR-MINFLUX imaging with nanometer localization precision. This work presents an overall strategy, where fluorophore blinking characterization and subsequent simulations make it possible to design optimal sample and excitation conditions, opening for NIR-MINFLUX imaging, as well as for a broader use of fluorophores in MINFLUX and related SRM studies.

Acknowledgements:

This study was supported by the European Union's Horizon 2020 research and innovation program under grant agreement 101017180 (NanoVIB).

 

Files with raw data on which the manuscript is based are grouped into folders according to the figures/tables in the manuscript where the extracted results are presented. Additionally, software developed and used for the simulations are arranged into a separate folder.
DOI: 10.5281/zenodo.15972470
title: Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking

description: This upload contains raw data and simulation software underlying the results presented in a manuscript submitted for peer-review, with the title:

 

Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking

 

Authored by:

C Venugopal Srambickal1,*, H Esmaeeli1,*, J Piguet1, L Reinkensmeier2, R Siegmund2, A Agostinho3 ,M Bates2, A Egner2, J Widengren1,**

1 Experimental Biomolecular Physics, Bio-Opto-Nano Unit, Department of Applied Physics, Royal Institute of Technology, SE-10691 Stockholm, Sweden

2 Department of Optical Nanoscopy, Institute for Nanophotonics, D-37077 Göttingen, Germany

3 Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-17165 Solna, Sweden

* Contributed equally

** Corresponding author: jwideng@kth.se

 

ABSTRACT

MINimal photon FLUXes (MINFLUX) offers super-resolution microscopy (SRM) with nanometer localization precision, with lower fluorophore brightness and photostability requirements than for other SRM techniques. Nonetheless, low localization probabilities have been reported in several MINFLUX studies, and a broader use of less bright and photostable fluorophores, including near-infrared (NIR) fluorophores has been difficult to realize. In this work, we identified fluorophore blinking as a main cause of erroneous (and dismissed) fluorophore localizations in MINFLUX imaging and devised strategies to overcome these effects. We systematically studied the blinking/switching properties of cyanine fluorophores emitting in the far-red or NIR range, over typical time scales (µs-10ms), sample and excitation conditions used in MINFLUX imaging. Subsequent simulations of representative MINFLUX localization procedures showed that trans-cis isomerization, and in particular photo-reduction of the fluorophores, can generate significant localization errors. These localization errors, however, could be suppressed by balanced redox buffers and repetitive excitation beam scans. Implementing these strategies, and replacing the slower, intrinsic switching of the fluorophores needed for the localization by transient binding of fluorophore-labelled DNA strands to complementary DNA strands attached to the targets (DNA-PAINT), we could for the first time demonstrate NIR-MINFLUX imaging with nanometer localization precision. This work presents an overall strategy, where fluorophore blinking characterization and subsequent simulations make it possible to design optimal sample and excitation conditions, opening for NIR-MINFLUX imaging, as well as for a broader use of fluorophores in MINFLUX and related SRM studies.

Acknowledgements:

This study was supported by the European Union's Horizon 2020 research and innovation program under grant agreement 101017180 (NanoVIB).

 

Files with raw data on which the manuscript is based are grouped into folders according to the figures/tables in the manuscript where the extracted results are presented. Additionally, software developed and used for the simulations are arranged into a separate folder.
DOI: 10.3535/m6e-mtl-5d3
title: Festuca Tourn. ex L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/ang-4c0-j3c
title: Arctotis L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/jyt-bwr-w8t
title: Habenaria linearifolia Maxim.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/hf7-0l1-9cq
title: Aegilops L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/p2n-pw4-jj5
title: Viola delphinantha Boiss.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/g9h-13r-xxm
title: Eriophorum scheuchzeri Hoppe

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/vcm-wev-vzt
title: Ulmus campestris L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/epl-55p-dmx
title: Ferula trachyphylla Rech. fil. & Riedl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/kve-0jm-c2b
title: Ferula pseudoalliacea Rech. fil.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/2qc-4v7-ytk
title: Acantholimon rhodopolium Rech. fil. & Schiman-Czeika

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/6k3-vx8-hwh
title: Ulex gallii Planch.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/jre-v65-hgv
title: Ferula trachyphylla Rech. fil. & Riedl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/8m0-zmg-1tl
title: Genista florida L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/bxv-hw6-ezv
title: Elymus repens (L.) Gould

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/72p-3lv-atv
title: Juniperus excelsa subsp. polycarpos (K.Koch) Takht.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/fdd-zr9-b92
title: Festuca valesiaca Schleich. ex Gaudin

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.15468/dl.qcfjxj
title: Occurrence Download

description: A dataset containing 100 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Scirtes, Naboandelus africanus, Ephydra, Halobates, Dicranomyia capicola, Dicranomyia, Dicranomyia tipulipes, Hydra oligactis, Hydrachna, Radix natalensis, Stratiomys, Tipula pomposa, Tricholeiochiton, Pseudovelia, Anopheles turkhudi, Trithemis kirbyi ardens, Africallagma elongatum, Paragordius, Potamocloeon, Cherax cainii, Coelometopon glenavoni, Neptosternus, Neptosternus alluaudi, Neptosternus meridianus, Neptosternus ornatus, Neptosternus pumicatus, Neptosternus resartus, Neptosternus simulator, Omaloplia, Phallocryptus, Biomphalaria glabrata, Gyraulus chinensis, Pomacea diffusa, Radix rubiginosa, Stempellinella, Acisoma panorpoides ascalaphoides, Attheyella, , Afrogyrus coretus, Microgomphus mozambicensis, Pirata, Rhyssemus, Tetragnatha, Parhydraena asperita, Agraphydrus albescens, Anacaena glabriventris, Sternolophus mundus, Aethionectes apicalis, Coelostoma, Agabus austellus, Mesoceration barriotum, Mesoceration integrum, Cantopelopia meilloni, Mesocyclops tenuisaccus, Alona bukobensis, Corophium triaenonyx, Amphiops lasioides, Aspidytes niobe, Berosus punctulatus, Bryophaenocladius brincki, Candonocypris, Chiltonia capensis, Chrysogaster, Cladopelma, Crenitis zimmermanni, Culex tigripes, Dicrotendipes sudanicus, Leydigia propinqua, Ochterus, Parakiefferiella ephippium, Parametriocnemus capensis, Paratrichocladius micans, Thiara amarula, Lymnaea truncatula)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((32.064 -28.9416,32.4847 -28.5786,30.5701 -28.5786,30.5701 -30.7329,30.6252 -30.6659,31.4179 -29.4257,31.8178 -29.0299,32.064 -28.9416))"
 ]
}

 The dataset includes 100 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067290-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/332-dct-nd5
title: Ferula rechingeri D. F. Chamb.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/m82-n2s-pf1
title: Salix alba L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/d6r-vmm-yht
title: Pulicaria odora (L.) Rchb.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/lc8-4c0-c6w
title: Festuca austrodolomitica Pils & Prosser

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/gq9-kga-wnx
title: Cerastium holosteoides Fr.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/k8j-jvr-vk4
title: Festuca Tourn. ex L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.15468/dl.y4veze
title: Occurrence Download

description: A dataset containing 199 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Occurrence evidence, Observation)",
 "TaxonKey is one of (Sylvia cantillans)",
 "Country is one of (Algeria, Angola, Benin, Botswana, British Indian Ocean Territory, Burkina Faso, Burundi, Cabo Verde, Cameroon, Central African Republic, Chad, Comoros, Congo, Congo, Democratic Republic of the, Côte d’Ivoire, Djibouti, Egypt, Equatorial Guinea, Eritrea, Eswatini, Ethiopia, French Southern Territories, Gabon, Gambia, Ghana, Guinea, Guinea-Bissau, Kenya, Lesotho, Liberia, Libya, Madagascar, Malawi, Mali, Mauritania, Mauritius, Mayotte, Morocco, Mozambique, Namibia, Niger, Nigeria, Réunion, Rwanda, Saint Helena, Ascension and Tristan da Cunha, Sao Tome and Principe, Senegal, Seychelles, Sierra Leone, Somalia, South Africa, South Sudan, Sudan, Tanzania, United Republic of, Togo, Tunisia, Uganda, Western Sahara, Zambia, Zimbabwe)"
 ]
}

 The dataset includes 199 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067292-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/dn1-m4k-1qe
title: Erysimum montosicola Jord.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/3vg-gve-gkr
title: Pimpinella barbata (DC.) Boiss.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/z3x-wvd-9dc
title: Calamagrostis epigejos (L.) Roth

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/bpz-dtr-xpt
title: Bromus danthoniae Trin.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/qqe-f99-l8j
title: Heliotropium macrolimbe Riedl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/jaf-1kx-9qe
title: Lindelofia anchusoides subsp. anchusoides

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/zt9-0r2-0wn
title: Arrhenatherum elatius (L.) P.Beauv. ex J.Presl & C.Presl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/lj3-q6e-91n
title: Robinia pseudoacacia L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/s5r-l46-5c3
title: Sigesbeckia orientalis L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.15468/dl.crzedg
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aegilops cylindrica",
 "Country is Algeria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067293-250717081556266/datasets/export for details.
DOI: 10.3535/6rj-qsc-r12
title: Acantholimon koelzii Rech. fil. & Köie

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/qe6-pdy-feq
title: Berkheya Ehrh.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.15468/dl.qv7ker
title: Occurrence Download

description: A dataset containing 35 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aegilops cylindrica",
 "Country is Austria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 35 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067294-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/c0e-vf8-sbr
title: Festuca Tourn. ex L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/k47-vt7-hbt
title: Heliotropium biannulatum Bunge

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/ccm-x3k-hhn
title: Poa variegata Lam.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/3l2-028-57w
title: Pilosella cymosa subsp. sabina (Sebast.) H. P. Fuchs

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/cm3-50t-a0g
title: Dalechampia ficifolia Lam.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.34740/kaggle/dsv/12609991
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.3535/tgh-sv8-pwt
title: Acantholimon leucochlorum Rech. fil. & Schiman-Czeika

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/xjc-g98-693
title: Acantholimon grammophyllum Rech. fil. & Köie

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/c7s-scf-8xa
title: Acantholimon distichum Rech. fil. & Schiman-Czeika

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/v19-f0g-z5b
title: Cirsium tuberosum (L.) All.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/j5w-9j3-ft6
title: Miconia fasciculata Gardner

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/mx6-72q-r1q
title: Festuca Tourn. ex L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/gqm-895-crq
title: Hedysarum minjanense Rech.f.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/slv-q4n-s6r
title: Alternanthera littoralis P. Beauv.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/3fd-9ek-kbb
title: Pimpinella diversifolia DC.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/gwl-1jt-5dl
title: Cerastium brachypetalum Desp. ex Pers.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/djl-h5v-fly
title: Chaenorhinum rubrifolium subsp. gerense (Stapf) D. A. Sutton

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.15468/dl.nxdjbg
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aegilops cylindrica",
 "Country is Belarus",
 "HasCoordinate is true"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067295-250717081556266/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/jph-xv6-52t
title: Turnera chamaedrifolia Cambess.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/prz-j7a-6r8
title: Sabulina foliosa (Royle ex Edgew. & Hook. fil.) Dillenb. & Kadereit

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/53p-71v-fyl
title: Chaenorhinum rubrifolium subsp. gerense (Stapf) D. A. Sutton

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/e96-l24-jn5
title: Hedysarum wakhanicum Podlech & O.Andersson

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/cnv-lk2-stl
title: Genista L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/bas-f6y-k1p
title: Agropyron Gaertn.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/rqe-ahh-y6s
title: Festuca valesiaca Schleich. ex Gaudin

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/29j-09s-x1q
title: Peucedanum p. min. p.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/53e-sy6-bpw
title: Potamogeton acutifolius Link

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/6tn-d0s-yv1
title: Acantholimon catenatum Rech. fil. & Schiman-Czeika

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/89d-9sw-pwv
title: Secale vavilovii Grossh.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/86r-6vp-db1
title: Hordeum murinum subsp. glaucum (Steud.) Tzvelev

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/bx2-bxf-0f5
title: Acantholimon carinatum Rech. fil. & Schiman-Czeika

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/997-64l-fm5
title: Taeniatherum caput-medusae (L.) Nevski

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/wwc-jd3-jk2
title: Elymus caninus (L.) L.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/3pn-kms-xt9
title: Tanacetum tenuissimum (Trautv.) Grossh.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/y1v-ahs-j42
title: Hedysarum bucharicum B.Fedtsch.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/2kd-6yw-gtj
title: Galium asperifolium Wall.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.5281/zenodo.16568275
title: Pseudoderbesia biodiversity: contigs and alignments
DOI: 10.3535/7tx-s8v-5kn
title: Hedysarum kopetdaghi Boriss.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/x8w-sdj-d16
title: Aegilops kotschyi Boiss.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/0hb-797-4fz
title: Byttneria jaculifolia Pohl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/pxy-lk1-vq7
title: Vellozia alutacea Pohl

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/tet-jt3-8cm
title: Hedysarum bucharicum B.Fedtsch.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/zgn-aex-d16
title: Hedysarum kopetdaghi Boriss.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/nay-44z-js5
title: Halopeplis pygmaea (Pall.) Bunge ex Ung.-Sternb.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/11h-frq-0bh
title: Humiriastrum dentatum (Casar.) Cuatrec.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/8ft-xby-ynt
title: Hedysarum bucharicum B.Fedtsch.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/f2y-g21-44d
title: Carex rigidioides (Gorodkov) V.I.Krecz.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/fye-prf-p35
title: Verbascum ikaricum Murb.

description: Digital Specimen for the physical specimen hosted at University of Graz.
DOI: 10.3535/da0-46w-z6j
title: Agrostis L.

description: Digital Specimen for the physical specimen hosted at University of Graz.