DOI: 10.5281/zenodo.14072069
title: A LexA-like repressor and global H-NS-like regulators enable the fine-tuning of R-tailocin expression in environmental Pseudomonas

description: This dataset is related to "A LexA-like repressor and global H-NS-like regulators enable the fine-tuning of R-tailocin expression in environmental Pseudomonas" and contains :



"RNA-seq_analyzed_dataset.xlsx": The processed RNA-seq data (related to Figures 1, 4 and Supplementary Figure 11). 

"Expression_data_analyzed.xlsx": The processed expression data (related to Figures 2, 3, Supplementary Figures 2, 3 and 10).

"Chip_seq_CHA0_I_CPM_normalized.bw": Bigwig file used for IGV visualization for wild type Pseudomonas protegens CHA0 under inducing conditions (related to Figures 2, 3, Supplementary Figures 6, 7, 8, 9, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_CHA0_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for wild type P. protegens CHA0 under non-inducing conditions (related to Figures 2, 3, Supplementary Figures 6, 7, 8, 9, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_prtR_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for the P. protegens CHA0 PrtR-V5 under inducing conditions (related to Figure 2, Supplementary Figures 7, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_prtR_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 PrtR-V5 under non-inducing conditions (related to Figure 2, Supplementary Figures 7, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_mvaT_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaT-V5 under inducing conditions (related to Figure 3, Supplementary Figures 7, 8, 9, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_mvaT_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaT-V5 under non-inducing conditions (related to Figure 3, Supplementary Figures 7, 8, 9, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_mvaV_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaV-V5 under inducing conditions (related to Figure 3, Supplementary Figures 7, 8, 9, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_mvaV_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaV-V5 under non-inducing conditions (related to Figure 3, Supplementary Figures 7, 8, 9, 12, 13, 14 and 15; reference genome: P. protegens CHA0 LS999205.1).

"GC_content.csv": Percentage of GC content in the different peaks identified in the ChIP-seq (related to Figure 3B).

"mvaT_mvaV_differences.xlsx": The processed data for the differences found in the RNA-seq between the double mutant ΔmvaTΔmvaV and wild type P. protegens CHA0 in non-induced condition and the double mutant ΔmvaTΔmvaV andwild type P. protegens CHA0 in induced condition (related to Figure 4).

"Supplementary_Figure_1AB_Expression.xlsx": The processed expression data (related to Supplementary Figure 1AB).

"Supplementary_Figure_1B_CFU.xlsx": The colony forming units obtained following exposure to the two inducing agents (mitomycin C and hydrogen peroxide; related to Supplementary Figure 1B)

"Supplementary_Figure_4.xlsx": The processed growth curve data for the mutants used for the ChIP-seq (related to Supplementary Figure 4).

"Chip_seq_parB_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 ParB-V5 under inducing conditions (related to Supplementary Figures 6 and 7; reference genome: P. protegens CHA0 LS999205.1).

"Chip_seq_parB_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 ParB-V5 under non-inducing conditions (related to Supplementary Figures 6 and 7; reference genome: P. protegens CHA0 LS999205.1).

"Supplementary_Figure_11_mvaT_mvaV_differences.xlsx": The processed data for the differences found in the RNA-seq between the single mutants ΔmvaT or ΔmvaV and wild type P. protegens CHA0 in non-induced condition and the single mutants ΔmvaT or ΔmvaV and wild type P. protegens CHA0 in induced condition (related to Supplementary Figure 11).