cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7422785
title: Supplementary Material for: A Systematic Review and Meta-Analysis of Molecular Biomarkers Associated with Early Neurological Deterioration Following Acute Stroke

description: <b><i>Background:</i></b> Early neurological deterioration (END) following acute stroke is associated with poorer long-term outcomes. Identification of patients at risk could assist early monitoring and treatment decisions. This review summarised the evidence describing non-radiological biomarkers for END. <b><i>Summary:</i></b> Electronic searches from January 1990 to March 2017 identified studies reporting a blood/cerebrospinal fluid (CSF)/urine biomarker measurement within 24 h of acute stroke and at least 2 serial assessments of clinical neurological status (&lt; 24 h and &lt; 7 days). Out of 12,895 citations, 82 studies were included, mostly focusing on ischaemic stroke. Using higher neurological thresholds, the n-weighted END incidence for ischaemic stroke was 11.9% (95% CI 11.4–12.4%) and 18.6% (17.9–19.2%) for lower thresholds. Incidence decreased with advancing study publication year (Pearson r-squared 0.23 and 0.15 for higher and lower threshold studies). After classification into 3 broad categories, meta-analysis showed that biomarkers associated with increased END risk (n; fixed-effects mean difference; 95% CI) were “metabolic” (glucose [<i>n</i> = 9,481; 0.90 mmol/L; 0.74–1.06], glycosylated haemoglobin [<i>n</i> = 3,146; 0.33%; 0.19–0.46], low-density lipoprotein [<i>n</i> = 4,839; 0.13 mmol/L; 0.06–0.21], total cholesterol [<i>n</i> = 4,762; 0.21 mmol/L; 0.11–0.31], triglycerides [<i>n</i> = 4,820; 0.11 mmol/L; 0.06–0.17], urea [<i>n</i> = 1,351; 0.55 mmol/L; 0.14–0.96], decreasing albumin [<i>n</i> = 513; 0.33 g/dL; 0.05–0.61]); “inflammatory and excitotoxic” (plasma glutamate [<i>n</i> = 688; 60.13 µmol/L; 50.04–70.22], CSF glutamate [<i>n</i> = 369; 7.50 µmol/L; 6.76–8.23], homocysteine [<i>n</i> = 824; 2.15 µmol/L; 0.68–3.61], leucocytes [<i>n</i> = 3,766; 0.54 × 10<sup>9</sup>/L; 0.34–0.74], high-sensitivity C-reactive protein [<i>n</i> = 1,707; 3.79 mg/L; 1.23–6.35]); and “coagulation/haematological” (fibrinogen [<i>n</i> = 3,132; 0.32 g/L; 0.25–0.40]; decreasing haemoglobin [<i>n</i> = 3,586; 2.38 g/L; 0.15–4.60]). <b><i>Key Messages:</i></b> Declining incidence of END may represent improving care standards; however, it remains a frequent occurrence. Although statistical associations exist between biomarkers and an increased risk of END, the most promising still need prospective evaluation to determine their additional value relative to baseline radiological and clinical characteristics.
DOI: 10.6084/m9.figshare.7422791
title: Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393

description: Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis. The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes. The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step. Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC). Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored. The majority of chondrocytes survived on all PLLA scaffolds functionalized with BG for the whole culture period. Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans. Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture. Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds. BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair. <b>Abbreviations</b>: <i>AB</i>: alcian blue ACAN: gene coding for aggrecan; BG: Bioactive glass; 2D: two-dimensional; 3D: three-dimensional; COL2A1: gene coding for type II collagen; DAPI: 4ʹ,6-diamidino-2-phenylindole; DMEM: Dulbecco’s Modified Eagle’s Medium; DMMB: dimethylmethylene blue; DSC: Differential scanning calorimetric analysis; ECM: extracellular matrix; EDTA: ethylenediaminetetraacetic acid; EtBr: ethidium bromide; FCS: fetal calf serum; FDA: fluorescein diacetate; GAG: glycosaminoglycans; HDPE: high density polyethylene; HE: hematoxylin and eosin staining; HCA: hydoxylapatite; PBE: phosphate buffered EDTA100 mM Na<sub>2</sub>HPO<sub>4</sub> and 5 mM EDTA, pH8; PBS: phosphate buffered saline; PFA: paraformaldehyde; PG: proteoglycans; PI: propidium iodide; PLLA: Poly-L-Lactic Acid Scaffold; RT: room temperature; SD: standard deviation; SEM: scanning electron microscopy; sGAG: sulfated glycosaminoglycans; SOX9/Sox9: SRY (sex-determining region Y)-box 9 protein; TBS: TRIS buffered saline; TIPS: Thermally Induced Phase Separation; XRD: X-ray diffraction analysis
DOI: 10.6084/m9.figshare.7422794
title: Distribution data and sources for 15 bryophyte species in Europe

description: Distribution data and sources for 15 bryophyte species in Europe
DOI: 10.6084/m9.figshare.7422794.v1
title: Distribution data and sources for 15 bryophyte species in Europe

description: Distribution data and sources for 15 bryophyte species in Europe
DOI: 10.7284/113012
title: Winch data collected during research cruise NH1313 using a Markey DUSH-5 instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7422791.v1
title: Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393

description: Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis. The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes. The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step. Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC). Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored. The majority of chondrocytes survived on all PLLA scaffolds functionalized with BG for the whole culture period. Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans. Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture. Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds. BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair. <b>Abbreviations</b>: <i>AB</i>: alcian blue ACAN: gene coding for aggrecan; BG: Bioactive glass; 2D: two-dimensional; 3D: three-dimensional; COL2A1: gene coding for type II collagen; DAPI: 4ʹ,6-diamidino-2-phenylindole; DMEM: Dulbecco’s Modified Eagle’s Medium; DMMB: dimethylmethylene blue; DSC: Differential scanning calorimetric analysis; ECM: extracellular matrix; EDTA: ethylenediaminetetraacetic acid; EtBr: ethidium bromide; FCS: fetal calf serum; FDA: fluorescein diacetate; GAG: glycosaminoglycans; HDPE: high density polyethylene; HE: hematoxylin and eosin staining; HCA: hydoxylapatite; PBE: phosphate buffered EDTA100 mM Na<sub>2</sub>HPO<sub>4</sub> and 5 mM EDTA, pH8; PBS: phosphate buffered saline; PFA: paraformaldehyde; PG: proteoglycans; PI: propidium iodide; PLLA: Poly-L-Lactic Acid Scaffold; RT: room temperature; SD: standard deviation; SEM: scanning electron microscopy; sGAG: sulfated glycosaminoglycans; SOX9/Sox9: SRY (sex-determining region Y)-box 9 protein; TBS: TRIS buffered saline; TIPS: Thermally Induced Phase Separation; XRD: X-ray diffraction analysis
DOI: 10.7284/113020
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise NH1314 using a Hawaii UHDAS instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7422677
title: Gut microbiome patterns correlate with higher postoperative complication rates after pancreatic surgery

description: The files in this dataset contains the biom file of the project as well as the metadata of the samples.<br>
DOI: 10.6084/m9.figshare.7422707
title: Approaches for the utilization of multiple criteria to select a working correlation structure for use within generalized estimating equations

description: Generalized estimating equations (GEE) incorporate a working correlation structure that is important because the more accurately this structure reflects the true structure, the more efficiently regression parameters may be estimated. Numerous criteria have therefore been proposed to select a working structure, although no criterion will always work better than all other criteria. In practice, it will be unknown which criterion will work best. Therefore, in this manuscript we propose how to utilize information from multiple criteria. We demonstrate the benefits of our proposed approach via a simulation study in a variety of settings and then in an application example.
DOI: 10.6084/m9.figshare.7422719
title: Supplementary Material for: Left-Sided Atrial Septal Pouch as a Risk Factor of Cryptogenic Stroke: A Systematic Review and Meta-Analysis

description: <b><i>Background:</i></b> Despite some evidence of left-sided septal pouch (LSSP) involvement in the pathogenesis of cardioembolic stroke, the question of LSSP clinical significance still remains unsolved. In this study, we aimed to determine the association between the LSSP presence and cryptogenic stroke using meta-analytical approach. <b><i>Methods:</i></b> We performed a systematic review of electronic databases for studies that compared the presence of LSSP in subjects with cryptogenic stroke and non-stroke control. Data were extracted and pooled into a meta-analysis. <b><i>Results:</i></b> Seven studies (400 cryptogenic stroke patients and 1,456 non-stroke controls) were included in the meta-analysis. A total of 138 LSSPs were identified among the cryptogenic stroke patients, with a pooled prevalence of 29.8% (95% CI 17.5–43.7%), and 268 LSSPs were identified in the non-stroke controls, with a pooled prevalence of 21.0% (95% CI 13.7–29.5%). After meta-analysis, the risk of cryptogenic stroke was higher in patients with an LSSP than in patients without LSSP (OR 1.52; 95% CI 1.15–2.00; <i>p</i> &lt; 0.001). No significant heterogeneity was detected across the included studies (<i>p</i> &gt; 0.05). <b><i>Conclusion:</i></b> Our meta-analysis demonstrated association between LSSP and cryptogenic stroke. In our univariate analysis, the risk of cryptogenic stroke is higher among patients with LSSP than in cases without the LSSP.
DOI: 10.6084/m9.figshare.7422677.v1
title: Gut microbiome patterns correlate with higher postoperative complication rates after pancreatic surgery

description: The files in this dataset contains the biom file of the project as well as the metadata of the samples.<br>
DOI: 10.6084/m9.figshare.7422707.v1
title: Approaches for the utilization of multiple criteria to select a working correlation structure for use within generalized estimating equations

description: Generalized estimating equations (GEE) incorporate a working correlation structure that is important because the more accurately this structure reflects the true structure, the more efficiently regression parameters may be estimated. Numerous criteria have therefore been proposed to select a working structure, although no criterion will always work better than all other criteria. In practice, it will be unknown which criterion will work best. Therefore, in this manuscript we propose how to utilize information from multiple criteria. We demonstrate the benefits of our proposed approach via a simulation study in a variety of settings and then in an application example.
DOI: 10.6084/m9.figshare.7422707.v2
title: Approaches for the utilization of multiple criteria to select a working correlation structure for use within generalized estimating equations

description: Generalized estimating equations (GEE) incorporate a working correlation structure that is important because the more accurately this structure reflects the true structure, the more efficiently regression parameters may be estimated. Numerous criteria have therefore been proposed to select a working structure, although no criterion will always work better than all other criteria. In practice, it will be unknown which criterion will work best. Therefore, in this manuscript we propose how to utilize information from multiple criteria. We demonstrate the benefits of our proposed approach via a simulation study in a variety of settings and then in an application example.
DOI: 10.6084/m9.figshare.7422719.v1
title: Supplementary Material for: Left-Sided Atrial Septal Pouch as a Risk Factor of Cryptogenic Stroke: A Systematic Review and Meta-Analysis

description: <b><i>Background:</i></b> Despite some evidence of left-sided septal pouch (LSSP) involvement in the pathogenesis of cardioembolic stroke, the question of LSSP clinical significance still remains unsolved. In this study, we aimed to determine the association between the LSSP presence and cryptogenic stroke using meta-analytical approach. <b><i>Methods:</i></b> We performed a systematic review of electronic databases for studies that compared the presence of LSSP in subjects with cryptogenic stroke and non-stroke control. Data were extracted and pooled into a meta-analysis. <b><i>Results:</i></b> Seven studies (400 cryptogenic stroke patients and 1,456 non-stroke controls) were included in the meta-analysis. A total of 138 LSSPs were identified among the cryptogenic stroke patients, with a pooled prevalence of 29.8% (95% CI 17.5–43.7%), and 268 LSSPs were identified in the non-stroke controls, with a pooled prevalence of 21.0% (95% CI 13.7–29.5%). After meta-analysis, the risk of cryptogenic stroke was higher in patients with an LSSP than in patients without LSSP (OR 1.52; 95% CI 1.15–2.00; <i>p</i> &lt; 0.001). No significant heterogeneity was detected across the included studies (<i>p</i> &gt; 0.05). <b><i>Conclusion:</i></b> Our meta-analysis demonstrated association between LSSP and cryptogenic stroke. In our univariate analysis, the risk of cryptogenic stroke is higher among patients with LSSP than in cases without the LSSP.
DOI: 10.6084/m9.figshare.7422638
title: Loss of functional connectivity is an early imaging marker in primary lateral sclerosis

description: <i>Objective:</i> The clinical diagnosis of primary lateral sclerosis can only be made after upper motor neuron symptoms have progressed for several years without developing lower motor neuron signs. The goal of the study was to identify neuroimaging changes that occur early in primary lateral sclerosis, prior to clinical diagnosis. <i>Methods:</i> MRI scans were obtained on 13 patients with adult-onset progressive spasticity for five years or less who were followed longitudinally to confirm a clinical diagnosis of primary lateral sclerosis. Resting state functional MRI, diffusion tensor imaging, and anatomical images were obtained. These “pre-PLS” patients were compared to 18 patients with longstanding, established primary lateral sclerosis and 28 controls. <i>Results:</i> Pre-PLS patients had a marked reduction in seed-based resting-state motor network connectivity compared to the controls and patients with longstanding disease. White matter regions with reduced fractional anisotropy were similar in the two patient groups compared to the controls. Patients with longstanding disease had cortical thinning of the precentral gyrus. A slight thinning of the right precentral gyrus was detected in initial pre-PLS patients’ scans. Follow-up scans in eight pre-PLS patients 1–2 years later showed increasing motor connectivity, thinning of the precentral gyrus, and no change in diffusion measures of the corticospinal tract or callosal motor region. <i>Conclusions:</i> Loss of motor functional connectivity is an early imaging marker in primary lateral sclerosis. This differs from literature descriptions of amyotrophic lateral sclerosis, warranting further studies to test whether resting-state functional MRI can differentiate between amyotrophic lateral sclerosis and primary lateral sclerosis at early disease stages.
DOI: 10.6084/m9.figshare.7422641
title: Relationship of statins and other cholesterol-lowering medications and risk of amyotrophic lateral sclerosis in the US elderly

description: <i>Objective</i>: Statins are commonly prescribed drugs that have been inconsistently associated with amyotrophic lateral sclerosis (ALS) risk. We examined associations between ALS risk and overall statin use, statin categories based on lipophilicity and other cholesterol-lowering medications, in Medicare beneficiaries. <i>Methods</i>: In <b>t</b>his nation-wide population-based case-control study, 10,450 Medicare participants (ages 66–89 years) diagnosed with ALS, using Medicare Parts A and B claims, between 1 January 2008 and 31 December 2014, were frequency-matched to 104,500 controls on age, sex, and selection year. Odds ratios (ORs) for the association between statins and ALS were estimated using logistic regression models. Covariates included dyslipidemia, other comorbidities, age, sex, race, proxies for smoking and obesity, Medicare use, and indicators of socioeconomic status. Statin use derived from Medicare Part D claims. Non–statin cholesterol–lowering drugs were evaluated as comparison drugs. <i>Results</i>: ALS risk was reduced with statin use (OR = 0.87 (95% confidence interval (CI) = 0.83–0.91)). While risk was unrelated to three cholesterol-lowering medications (nitrates, bile acid sequestrants, and ezetimibe), it was associated with fibrates (OR = 0.88 (95% CI = 0.80–0.97)). Risk for lipophilic statins was slightly lower than for other statins. ALS risk was lower in all statin categories for dyslipidemic individuals, but only lipophilic statins were associated with lower risk in non-dyslipidemic individuals and demonstrated an inverse trend with duration. <i>Conclusions</i>: Our findings suggest that statins are associated with lower ALS risk and offer new evidence that fibrates may be related to lower risk. However, we were unable to fully adjust for smoking and body mass index.
DOI: 10.7284/113023
title: GNSS (Global Navigation Satellite System) data collected during research cruise NH1314 using a Furuno GP-150 instrument system onboard the platform RV New Horizon

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7422638.v1
title: Loss of functional connectivity is an early imaging marker in primary lateral sclerosis

description: <i>Objective:</i> The clinical diagnosis of primary lateral sclerosis can only be made after upper motor neuron symptoms have progressed for several years without developing lower motor neuron signs. The goal of the study was to identify neuroimaging changes that occur early in primary lateral sclerosis, prior to clinical diagnosis. <i>Methods:</i> MRI scans were obtained on 13 patients with adult-onset progressive spasticity for five years or less who were followed longitudinally to confirm a clinical diagnosis of primary lateral sclerosis. Resting state functional MRI, diffusion tensor imaging, and anatomical images were obtained. These “pre-PLS” patients were compared to 18 patients with longstanding, established primary lateral sclerosis and 28 controls. <i>Results:</i> Pre-PLS patients had a marked reduction in seed-based resting-state motor network connectivity compared to the controls and patients with longstanding disease. White matter regions with reduced fractional anisotropy were similar in the two patient groups compared to the controls. Patients with longstanding disease had cortical thinning of the precentral gyrus. A slight thinning of the right precentral gyrus was detected in initial pre-PLS patients’ scans. Follow-up scans in eight pre-PLS patients 1–2 years later showed increasing motor connectivity, thinning of the precentral gyrus, and no change in diffusion measures of the corticospinal tract or callosal motor region. <i>Conclusions:</i> Loss of motor functional connectivity is an early imaging marker in primary lateral sclerosis. This differs from literature descriptions of amyotrophic lateral sclerosis, warranting further studies to test whether resting-state functional MRI can differentiate between amyotrophic lateral sclerosis and primary lateral sclerosis at early disease stages.