cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5286/isis.e.rb1920382-1
title: Hyaluronic acid-based nanogels encapsulating cell-penetrating peptides and insulin

description: In this proposal we want to characterize the physico-chemical properties of self-assembling nanogels composed of octenyl-succinic anhydride modified hyaluronic acid (OSA-HA) encapsulating cell-penetrating peptides (CPPs). CPPs are short amino acid chains (10-50 AAs) which have shown to enhance transport of cargos, such as insulin into or across cells. We have assessed the formation of nanogels with a lipophilic antibiotic and an antimicrobial peptide and have noticed a difference in structural organization, but we still lack fundamental understanding on what causes the changes in the structure of the nanogels upon drug loading. We would like to use a range of 12 peptides - from more cationic to neutral and more hydrophilic to more hydrophobic - for nanogel formulation and assess the changes in nanogel structure as a result of peptide charge and hydrophobicity.
DOI: 10.14457/cu.the.2009.1579
title: Comparison of fault current levels between grid-connected photovoltaic system and synchronous machine distributed generation in a distribution system

description: This thesis presents the comparison of fault current levels between grid-connected photovoltaic (GCPV) system and synchronous machine distributed generator (SG). Their impacts are studied using 23-bus, 65MVAsc, 23kV distribution system with the total load of 700W. Modeling of the GCPV is based on a PV model described by Shockley diode equation with a series resistance, a boost converter with maximum power point tracking (MPPT) technique, and a pulse width modulation (PWM) inverter with a voltage regulator. The siulation is carried out using the MATLAB-SIMULINK. Both GCPV and SG with various penetration levels are installed at various buses in the system. Various fault positions are assumed in order to assess the fault current contributon from the GCPV system to the man grid by comparing with the fault current contribution from the SG. Result comparison between the system with GCPV installaton and SG installation is discussed. Impacts of fault current contribution on protection coordination are considered based on IEEE Std C37.112-1996.
DOI: 10.14457/cu.the.2009.1580
title: Biodegradation of 17 alpha-methyltestosterone by microorganisms in sediment from masculinizing pond of nile tilapia fry

description: 17alpha-methyltestosterone (MT), an anabolic androgenic steroid, is used widely in reversing the sex of Nile tilapia (Oreochromis niloticus), an important freshwater aquaculture fish in many developing countries, to produce an all male population. MT is a suspect human carcinogen and can act as an endocrine disrupting compound interfering with the normal function of endocrine and reproductive systems of animals when exposed to MT levels as low as nanogram per liter. However, an understanding of the occurrence and fate of MT in the environment is very limited. This study investigated the biodegradation of MT and its androgenic potency by microorganisms in sediment under different electron acceptor conditions and by isolated MT-degrading bacteria from the sediment and water of a masculinizing pond. MT was degraded under aerobic, sulfate-reducing and methanogenic conditions, but it was hardly degraded under iron(III)-reducing condition and was not degraded under nitrate-reducing condition. Statistically, the first-order biodegradation rates of MT for aerobic, sulfate-reducing and methanogenic conditions were similar. However, the measured androgenic activities under methanogenic condition continued to persist for more than one month even though the measured MT concentrations were negligible. An unknown labile metabolite of MT was found under aerobic condition but not for other electron acceptor conditions. Based on the biodegradation results, it appeared that MT and its metabolites can potentially accumulate in environments under iron (III)-reducing, nitratereducing, and methanogenic conditions. Thirty strains of MT-degrading bacteria were isolated from the sediment and water of masculinization pond. The isolated bacteria were related to three genus including Pimelobacter, Rhodococcus, and Nocardioides. Three isolated strains, strains SB100-05, SB010-03, and WB100-05, were selected for further identification and characterization. Phylogenetic analysis, physiological properties and cell morphology of the three strains SB100-05, SB010-03 and WB100-05 suggested that they were closely related to Nocardioides aromaticivorans, Rhodococcus equi, and Nocardioides nitrophenolicus, respectively. Haldane’s model was found to fit the MT degradation kinetics of the three strains indicating the possibility of substrate inhibition. Of the three strains tested, strain SB10005 degraded MT faster but had lower resistance to high MT concentration than the other strains.
DOI: 10.14457/cu.the.2009.1581
title: Effects of chemical crosslinking and hydroxyapatite on the properties of Thai silk fibroin/gelatin scaffolds

description: This research aimed to investigate the effects of chemical crosslinking and hydroxyapatite on the properties of Thai silk fibroin/gelatin scaffolds. Crosslinked Thai silk fibroin/gelatin scaffolds were prepared by directly adding EDC/NHS in blended solution and subsequently fabricating via freeze-drying method. The effect of the weight blending ratios of Thai silk fibroin/gelatin was examined. It was found that all Thai silk fibroin/gelatin scaffolds possessed uniform porous structure. Crosslinked Thai silk fibroin/gelatin scaffolds showed lower weight loss (%) and higher compressive modulus than those of non-crosslinked scaffolds. Interestingly, non-crosslinked Thai silk fibroin/gelatin scaffold at the weight blending ratio of 50/50 showed the lowest weight loss (%) with excellent mechanical strength as good as the crosslinked scaffold due to the suitable electrostatic interactions between silk fibroin and gelatin. The results on in vitro biodegradability using collagenase solution revealed that Thai silk fibroin and crosslinking could delay the biodegradability of scaffolds. XRD results proved that all Thai silk fibroin/gelatin scaffolds before and after biodegradation possessed similar amorphous structure. The results on in vitro cell culture using bone marrow-derived stem cells showed that Thai silk fibroin/gelatin scaffolds containing high amount of gelatin were more effective to promote cell proliferation. Furthermore, in the study of the effect of hydroxyapatite adding, Thai silk fibroin/gelatin scaffolds were added with hydroxyapatite using homogenization method. Homogeneous distribution of hydroxyapatite granules in the scaffolds was observed. Homogenized Thai silk fibroin/gelatin scaffolds with and without hydroxyapatite incorporation did not support proliferation. This might be the result of mass transfer limit and the toxicity of chloroform residue in scaffolds. The result of osteogenic differentiation suggested that osteoconductive potential of both scaffolds was similar. However, these should be investigated and clarified in further study to obtain suitable scaffolds for tissue engineering application.
DOI: 10.14457/cu.the.2009.1582
title: Effects of whey protein supplementation on clinical outcomes and insulin resistance in type 2 diabetic outpatients at public health center 66, health department, Bangkok metropolitan administration

description: This study examined the effects of whey protein isolate (WPI) supplementation on clinical outcomes and insulin resistance in type 2 diabetic patients at Public Health Center 66. The subjects (n = 36) received nutrition counseling and were randomly assigned into 2 groups: treatment group supplemented with 30 gram per day of WPI for 6 weeks and control group. Fasting blood biochemistry, anthropometry, and blood pressure were examined at baseline and week 6 of the study. The results demonstrated that at week 6 of the study the levels of glucose, insulin, glycosylated hemoglobin HbA1c , insulin resistance assessed by the homeostasis model assessment’method , total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol did not change from baselines in both groups, and no significant differences between groups were observed. However, after WPI supplementation the subjects in the treatment group had significant reduction in triglyceride levels (p = 0.043), systolic blood pressure (p = 0.002), body weight (p = 0.043), and body mass index (p = 0.043), when compared with baselines, but there were no significant differences in these parameters between groups. WPI was generally well tolerated by most subjects, and no serious adverse effects were observed throughout the supplementation period. Three of eighteen subjects (16.67%) had mild flatulence during WPI supplementation. Blood urea nitrogen (BUN) levels were significantly increased in the treatment group when compared with baseline and between groups, whereas serum creatinine level did not change. These suggested that an increase in BUN might be due to higher protein intake during the supplementation period. This study indicated that short-term WPI supplementation did not affect blood glucose level and insulin resistance in type 2 diabetic patients. However, it may be beneficial for controlling of blood triglyceride, blood pressure and body weight.
DOI: 10.14457/cu.the.2009.1583
title: Nasal delivery of japanese encephalitis vaccine using modified poly (D,L-Lactic -co-Glycolic acid) particles as delivery vehicle

description: Novel carriers for nasal application of Japanese encephalitis (JE) vaccine utilized by poly(D,L lactic-co-glycolic acid) (PLGA) and surface conjugating with chitosan (CS) and Al(OH)₃ were prepared by means of multiple emulsion solvent evaporation technique to obtain various sizes and surface charge of particles. The influences of preparation conditions on physicochemical characteristics and on integrity of JE virus were evaluated. The results demonstrated that size and uniformity increased by increasing primary w/o ratio, secondary w/o/w ratio, volume of PVA or by decreasing the percentage of PVA as well as the sonication output. All microparticles were spherical with smooth surface comprising the positively surfaced charge after conjugating with CS and Al(OH)₃. Integrity and structural conformation of epitope E protein was fine retained during the preparation process. The efficacy of these particles as nasal JE vaccine carrier whose particles diameter and surface charge were suitable for nasal route was first introduced in this study. The use of various size of PLGA, PLGA conjugated CS and Al(OH)₃ as nasal JE vaccine carrier was investigated after application into porcine nasal mucosa. CLSM suggested that particulate uptake of particles up to approximately 1µm was rapidly taken up by and permeated through nasal mucosa even though the adhesion on tissue surface was relatively lower compared to those from 5 and 15 µm. The tissue adhesion was considerably improved after the conjugation of 1 µm particles with CS and Al(OH)₃ However, higher amount of particulate uptake and permeation occurred only in Al(OH)₃ formulation. CS formulation showed conversely different result as the uptake mechanism of CS was mainly by paracellular path, consequence in the lower percentage of particulate uptake but the higher percentage of particulate permeation within the same period of time since paracellular process was a more rapid process than transcellular pathway. All formulations selected to further study in experimental animal were relatively non-toxic to nasal tissue. Induction of nasal immune response following nasal administration of various dose of JE vaccine and JE vaccine entrapped in different size and surface charge of PLGA particles was evaluated. The immunogenicity of 10,40 and 80µg dose of JE in Balb/C mice was relatively equal but considerably lower compared to subcutaneous route with the same frequency of administration. The 10µg dose entrapped in 1,5 and 15µm PLGA particles was selected to inoculate in Balb/C mice and found that particles could enhance more level of immunological titer compared to vaccine alone and 1µm could elicit the highest level but still not comparable to subcutaneous route and repeated vaccination dose remained necessitate. After conjugation by CS and Al(OH)₃, the titer seemed to persist for a longer period of time with much higher level compared to unconjugated particles and the booster dose need not be required.
DOI: 10.14457/cu.the.2009.1584
title: Preparation of Thai silk fibroin/gelatin electrospun fiber mats for controlled release applications

description: This research aimed to study the preparation of fiber mats from Thai silk fibroin/type B gelatin (SF/GB) using electrospinning for controlled release applications. From the results, the increasing in applied voltage resulted in narrow fiber size distribution and decreased average fiber size. An increasing in silk fibroin content in blended solution also resulted in larger size of obtained electrospun fiber. A smooth fiber could be produced from SF/GB blended solution at weight blending ratios of 10/90, 20/80, 30/70, 40/60, and 50/50. Therefore, the blended SF/GB fiber mats at the weight ratio of 50/50, 30/70 and 10/90 were selected to prevent the water solubility of the fiber mats by crosslinking treatment. Among three treatment methods, spraying EDC/NHS dissolved in ethanol solution onto fiber mats following by soaking the fiber mats in that solution was a suitable treatment method because the blended fiber mats after treatment showed the lowest percentage of weight loss and the morphology similar to its original. Moreover, the blended fiber mats with high silk fibroin content showed good mechanical properties compared to those with low silk fibroin content. From in vitro biodegradation, SF/GB 10/90 fiber mats was rapidly degraded in collagenase solution because the structure of gelatin was the same as collagen that can be degraded by collagenase. From in vitro controlled release of model compounds from SF/GB blended fiber mats, it was expected that the same charge of blended fiber mats and azo-casein would result in the repulsion force. Azo-casein was continuously diffused from blended fiber mats within 72-h of incubation. In contrast, methylene blue and nerve growth factor could be absorbed by an attraction interaction between the blended fiber mats and the two model compounds. The release of these of model compounds could be controlled by the biodegradation of SF/GB blended fiber mats.
DOI: 10.14457/cu.the.2009.1585
title: Selection of highly variable chloroplast DNA sequences for genetic relationship study of tobacco cultivars in Thailand

description: In Thailand, imported and local tobacco cultivars have different regulations in tariff collection. However, there are major legal and technical problems on how to distinguish between the two groups of tobacco cultivars. In this study, highly-variable noncoding regions of chloroplast DNA was ntroduced to study genetic relatonships among totally 51 local and imported tobacco cultvars (Virginia, Turkish, and Burley cultivar groups) grown in Thailand. The amplification and sequencing results revealed that six out of nine regions (rp/32-trnL, ndhC-trnV, ndhF-rpl32, psbD-trnT, psbJ-petA and atpl-atpH) showed polymorphic sequence characteristcs, both in sequence engths and the amounts of base usbstitutions. Flmost all of Virginia cultivars were different in nucleotide sequence from the other cultivars. rpl32-trnL region could also distinguish seven local cultivars and one roll-your-own tobacco product with a large 66 bp insertion. The sequence polymorphism of rpl32-trnL region was in the highest vaue with 10.47% variability and 75 potentially informative characters. A multiplex PCR technique was successfully developed to distinguish Virginia cultivars and the seven special local cultivars from the others. This marker can be used as a solution for the "local" and "imported" cultivar problem.
DOI: 10.14457/cu.the.2009.1586
title: Prospects and policy options for Thailand's trade in bioenergy in the Mekong : implications for sustainable development

description: This thesis examines the emerging dynamics of agroenergy, sustainable development and regional integration in the Mekong region, where trade has become the currency of cooperation. Thailand has commercialised biofuels to contribute to the national target to increase renewable energy sources to 8% by 2011. Whilst Thailand has signalled a new era with its renewable energy strategies, there has yet to be sufficient assessment of the socio–environmental effects of the agricultural shifts entailed. The conceptual framework for this thesis sets the Mekong agroenergy discourse in the context of the theories of reflexive modernisation and regional modernities. It examines agroenergy development in theory, policy and practice through empirical analysis based on field study of six cases, indepth interviews, focus groups and a survey questionnaire, with Brazil’s agroenergy experience as a comparative model. This work elaborates a new interpretation of modernsation based on an agroecological framework that incorporates smallholders in the regional energy matrix. The main conclusions are that Thailand’s bioenergy model has the potential to diversify and add value to its agricultural production and trade while addressing sustainable development. However, increasing demand for agricultural feedstocks, such as cassava and sugarcane for ethanol and palm oil for biodiesel, may encourage unsustainable agricultural expansion in neighbouring Mekong countries. There is a rupture in policy making between different stakeholders that needs to be addressed by coordinated change towards sustainable development that integrates smallholders and avoids business as usual scenarios driving large–scale monoculture plantations.
DOI: 10.15468/oysur7
title: Review of genera of Sciomyzidae (Diptera: Acalyptratae) from China, with new records, synonyms, and notes on distribution

description: This dataset contains the digitized treatments in Plazi based on the original journal article Li, Zhu, Yang, Ding, Murphy, William L. (2019): Review of genera of Sciomyzidae (Diptera: Acalyptratae) from China, with new records, synonyms, and notes on distribution. Zootaxa 4656 (1): 71-98, DOI: https://doi.org/10.11646/zootaxa.4656.1.3
DOI: 10.5286/isis.e.rb2000023
title: SANS studies of Physiogel creams

description: Aqueous creams are widely used to treat skin conditions like rosacea, dermatitis and psoriasis.They are very complex chemical mixtures, and our knowledge of their molecular structure, and the relationship between this and their stability and clinical benefit to patients is sadly lacking. The investigations to be performed here (using the technique of small angle neutron scattering) aim to determine the molecular structure of aqueous creams designed to model the GlaxoSmithKline Physiogel(TM) range of skin care products. A series of these cream formulations will be studied, each having a single deuterium-labelled constituent, so that we can then determine how the constituents are arranged, and thereby deduce the cream structure. By this means we hope to gain a better understanding of how the formulation of such creams might be modified to give improved and more efficacious skin care produc
DOI: 10.5286/isis.e.rb2000023-1
title: SANS studies of Physiogel creams

description: Aqueous creams are widely used to treat skin conditions like rosacea, dermatitis and psoriasis.They are very complex chemical mixtures, and our knowledge of their molecular structure, and the relationship between this and their stability and clinical benefit to patients is sadly lacking. The investigations to be performed here (using the technique of small angle neutron scattering) aim to determine the molecular structure of aqueous creams designed to model the GlaxoSmithKline Physiogel(TM) range of skin care products. A series of these cream formulations will be studied, each having a single deuterium-labelled constituent, so that we can then determine how the constituents are arranged, and thereby deduce the cream structure. By this means we hope to gain a better understanding of how the formulation of such creams might be modified to give improved and more efficacious skin care produc
DOI: 10.14457/cu.the.2009.1587
title: Synthesis of cationic hyperbranched polyamidoamine dendrimer and comparison of its antimicrobal activity with chitosan

description: The cationic hyperbranched dendritic polyamidoamine (PAMAM) containing terminal methyl ester end groups was synthesized and employed for modifying bulk chitosan. This modifying agent was synthesized using the repetitive reactions between Michael addition and amidation to obtain the methyl ester group terminated hyperbranched PAMAM and then followed by the methylaton of methyl ester terminated hyperbranched dendritic PAMAM (PAMAM-ester) with dimethyl sulphate. Hyperbranched dendrtic PAMAM and cationic hyperbranched dendritic PAMAM were characterized by FTIR and H NMR analysis to confirm these structures. The modification of chitosan with catonic hyperbranched PAMAM-ester was carried-out through the reaction of the polyamidoamine methyl ester end group and the chitosan amine group at room temperature for 5 days. The amide linkage was formed and evidenced by FTIR analysis. Chitosan modfied with excess amount of catonic hyperbranched PAMAM-ester was water soluble at neutral pH. TGA and XRD analyses provided evidence that the catonic hyperbranched PAMAM-chitosan containing a bulky side group exhibited enhanced chain mobility, resultng n increasing water solubility. Pure chitosan and cationic hyperbranched dendritic PAMAM-chitosan films showed excellent antimicrobial activity. However, coating of bulk chitosan and in-situ depolymerization of coated chitosan show that chitosan-fabrics showed negative antimicrobial result. On other hand, cotton fabrics treated with cationic hyperbranched dendritic PAMAM have good ability to inhibit the microbial growth. Combined treatment of cotton fabric with chitosan and cationic hyperbranched dendritic PAMAM indicated that cationic hyperbranched dendritic PAMAM moiety played a synergistic effect on antimicrobial activity due to its cationic characterstic.