DOI: 10.26181/25241788.v2
title: Variability in the dual mycorrhizal associations of tea tree, <i>Melaleuca alternifolia</i>

description: This repository contains eight packed data files.The quality-filtered otu tables and taxonomic annotation tables for arbuscular mycorrhizal fungi and ectomycorrhizal fungi, as well as the sample metadata sheet, can be used to run the reproducible statistical analysis workflow at GitHub.Contents:The<b>BLAST_best_10.csv</b>file contains the following information:OTU_ID: OTU identifier abundance: total OTU read abundancereference: UNITE database reference sequence idkingdom: UNITE kingdom annotationphylum: UNITE phylum annotationclass: UNITE class annotationorder: UNITE order annotationfamily: UNITE family annotationgenus: UNITE genus annotationspecies: UNITE species hypothesis annotationpident: per cent sequence similarity to the reference sequencelength: alignment length of the query to the reference sequence (sequence overlap)slen: query sequence lengthmismatches: number of mismatches to the reference sequencegapopen: number of gap openings relative to the reference sequenceqstart: first position of alignment of the query sequence to the reference sequenceqend: last position of alignment of the query sequence to the reference sequencesstart: first position of alignment of the reference sequencesend: last position of alignment of the reference sequenceevalue: e-value of the query sequence to the best-hitting reference sequencebitscore: score of the blast match of the query sequence to the reference sequence<pre></pre>The<b>taxa_AM.csv</b>and<b>taxa_ECM.csv</b>files contain the following information (consensus similarity is 66.67% across the five best hits; coverage threshold is 90%; similarity thresholds are defined at level genus for AM fungi and ectomycorrhiza lineage for ECM fungi):<br>OTU_ID: OTU identifier kingdom: kingdom annotation based on consensus hit and similarity and coverage thresholdsphylum: phylum annotation based on consensus hit and similarity and coverage thresholdsclass: class annotation based on consensus hit and similarity and coverage thresholdsorder: order annotation based on consensus hit and similarity and coverage thresholdsfamily: family annotation based on consensus hit and similarity and coverage thresholdsgenus: genus annotation based on consensus hit and similarity and coverage thresholdsspecies: genus annotation based on consensus hit and similarity and coverage thresholdstaxa_consensus: per cent consensus of BLAST hit across the five best hitsabundance: total OTU read abundancesimilarity: per cent sequence similarity to the consensus sequencecoverage: per cent sequence coverage to the consensus sequenceevalue: evalue to the consensus sequenceectomycorrhiza_exploration_type: inferred lineage of ectomycorrhizal fungi based on the FungalTraits databaseectomycorrhiza_lineage: inferred ectomycorrhiza exploration type based on the FungalTraits databaseThe<b>metadata.csv</b>file contains the following information:sample: sample IDecotype: tea tree ecotypesite: site IDcol_AM: number of arbuscular mycorhizal observations per 60 cm of fine rootcol_ECM: number of ectomycorrhizal observations per root tips countedweight: number of total mycorrhizal observations per samplelatitude: latitude in decimal degreeslongitude: longitude in decimal degreesMAP: mean annual precipitation (°C)MAT: mean annual temperatre (mm)soil_moisture: estimate of site level soil moisture content (%)pH: estimate of site level soil pH (1:5 water)C_total: estimate of site level total soil organic carbon (%)N_total: estimate of site level total soil nitrogen (%)P_available: estimate of site level soil plant-avalable phosphorus (mg/kg; Colwell extraction method)ammonium: estimate of site level soil ammonium (mg/kg; KCl extraction)nitrate: estimate of site level soil nitrate (mg/kg; KCl extraction)