DOI: 10.22002/x7d70-6v630
title: HUVECs CHMP4A+VPS4DN

description: HUVECs cells transfected with CHMP4A+VPS4DN constructs using Nucleofector Kit. Sample# C58-2
description: <strong>Tilt Series Date:</strong> 2011-03-31</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4DN construct provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-03-31-17/3dimage_68433/</p>11mar31a_C_58-2_021_full.rec,  Reconstruction (Pixel Size 2.0 nm), 469.8 MB  
        
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        11mar31a_C_58-2_021.st,  Tilt Series (Pixel Size 0.5 nm), 255.9 MB  
        
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        11mar31a_C_58-2_021_vaultovrview.tiff,  file associated to 3D Image #68433, 1.2 MB  
        
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        11mar31a_C_58-2_021_vault3ovrview.tiff,  file associated to 3D Image #68433, 1.2 MB  
        
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        11mar31a_C_58-2_021_vault3.tiff,  file associated to 3D Image #68433, 759.5 kB  
        
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        11mar31a_C_58-2_021_vault2ovrview.tiff,  file associated to 3D Image #68433, 1.1 MB  
        
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        11mar31a_C_58-2_021_vault.tiff,  file associated to 3D Image #68433, 830.1 kB  
        
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