DOI: 10.5061/dryad.q9080
title: Data from: Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions

description: All diseases are characterized by distinct changes in tissue molecular
 distribution. Molecular analysis of intact tissues traditionally requires
 pre-existing knowledge of, and reagents for, the targets of interest.
 Conversely, label-free discovery of disease-associated tissue analytes
 requires destructive processing for downstream identification platforms.
 Tissue-based analyses therefore sacrifice discovery to gain spatial
 distribution of known targets, or sacrifice tissue architecture for
 discovery of unknown targets. To overcome these obstacles, we developed a
 multi-modality imaging platform for discovery-based molecular histology.
 We apply this platform to a model of disseminated infection triggered by
 the important pathogen Staphylococcus aureus, leading to the discovery of
 infection-associated alterations in the distribution and abundance of
 proteins and elements in tissue. These data provide an unbiased,
 three-dimensional analysis of how disease impacts the molecular
 architecture of complex tissues, enable culture-free diagnosis of
 infection through imaging-based detection of bacterial and host analytes,
 and reveal molecular heterogeneity at the host-pathogen interface.
description: ICP 3D
 VolumeThese data are LA-ICP
 IMS data from a mouse kidney infected with S. aureus. Each section of the
 mouse is numbered and data within each folder correspond to each
 section.ICP Iron
 ReporterThese data are LA-ICP
 IMS data corresponding to mouse kidneys infected with S.aureus. These mice
 were infected with a, iron-reporter. Mice were analyzed at three time
 points post infection: 4, 7, and 10 daysICP Mn
 ReporterThese are LA-ICP IMS
 data from the kidney of mice infected with S. aureus. This stain of S.
 aureus is responsive to manganese. Mice were analyzed at 4, 7, and 10 days
 post infection.bottom
 upThis file represents
 LC-MS/MS targeted analysis of a peptide detected in MALDI IMS data, delta
 hemolysin. The sample is a fraction purified from mouse kidney infected
 with S. aureus. The samples was tryptically digested prior to analysis
 with LC-MS/MSBottom
 up.zipFTICRMALDI FTICR IMS of a
 mouse section containing an infectious foci. The FTICR was used to obtain
 accurate mass measurements from the MALDI IMS data in order to have
 confidence in protein identifications.MALDI 3D
 VolumeThese data are MALDI IMS
 data from a 3D mouse kidney infected with S. aureus. These were collected
 using a SimulTOF mass spectrometer.Top
 DownThese data represent
 LC-MS/MS analysis of intact proteins directly extracted from infectious
 foci of mouse kidneys infected with S. aureus. Proteins were fragmented
 using ETD, and ions matching ions detected in MALDI IMS data were manually
 sequenced.MALDI Iron 4
 DayThese data represent
 MALDI IMS data of biological replicates of mice infected with S. aureus 4
 days post infection. This particular strain of S. aureus has been modified
 with a fluorescent reporter sensitive to iron deplete conditions. Three
 biological replicates are tested here.MALDI Fe Day
 4.zipMALDI Iron 7
 DayThese data represent
 MALDI IMS data of biological replicates of mice infected with S. aureus 7
 days post infection. This particular strain of S. aureus has been modified
 with a fluorescent reporter sensitive to iron deplete conditions. Three
 biological replicates are tested here.MALDI Fe Day
 7.zipMALDI Iron 10
 DayThese data represent
 MALDI IMS data of biological replicates of mice infected with S. aureus 10
 days post infection. This particular strain of S. aureus has been modified
 with a fluorescent reporter sensitive to iron deplete conditions. Three
 biological replicates are tested here.MALDI
 Fe_10DPI.zipMALDI manganese 4
 DayThese data represent
 MALDI IMS data of biological replicates of mice infected with S. aureus 4
 days post infection. This particular strain of S. aureus has been modified
 with a fluorescent reporter sensitive to manganese deplete conditions.
 Three biological replicates are tested here.MALDI
 Mn_day4.zipMALDI manganese 7
 DayThese data represent
 MALDI IMS data of biological replicates of mice infected with S. aureus 7
 days post infection. This particular strain of S. aureus has been modified
 with a fluorescent reporter sensitive to manganese deplete conditions.
 Three biological replicates are tested here.MALDI
 Mn_day7.zipMALDI manganese 10
 DayThese data represent
 MALDI IMS data of biological replicates of mice infected with S. aureus 4
 days post infection. This particular strain of S. aureus has been modified
 with a fluorescent reporter sensitive to manganese deplete conditions.
 Three biological replicates are tested here.MALDI
 Mn_10DPI.zip