DOI: 10.5061/dryad.cp40s
title: Data from: A migratory divide in the painted bunting (Passerina ciris)

description: In the Painted Bunting (Passerina ciris), a North American songbird,
 populations on the Atlantic coast and interior southern United States are
 known to be allopatric during the breeding season, but efforts to map
 connectivity with wintering ranges have been largely inconclusive. Using
 genomic and morphological data from museum specimens and banded birds, we
 found evidence of three genetically differentiated Painted Bunting
 populations with distinct wintering ranges and molt-migration phenologies.
 In addition to confirming that the Atlantic coast population remains
 allopatric throughout the annual cycle, we identified an unexpected
 migratory divide within the interior breeding range. Populations breeding
 in Louisiana winter on the Yucatán Peninsula, and are parapatric with
 other interior populations that winter in mainland Mexico and Central
 America. Across the interior breeding range, genetic ancestry is also
 associated with variation in wing length, suggesting that selection may be
 promoting morphological divergence in populations with different migration
 strategies.
description: Pciris_mtDNA_ND2ND2 sequence alignment in phylip formatPciris_ND2.phyR_scriptsR scripts used for multivariate genomic analysis, mtDNA analysis, morphology, dadi data preparation, dadi model to demographic value conversion, and all figures.specimen_dataMuseum and band numbers for specimens analyzed in this study, plus wing length and tarsus measurements for interior samples.ddRAD_assemblyddRAD sequence assembly in vcf and structure format, and pyRAD sequence assembly parameters file.dadidemographic analysis (dadi) python scripts and dadi-formatted input file.Pciris_reads_1of6100bp SR sequence reads from an illumina Hiseq 2500 for Passerina ciris. Library prepped via ddRAD protocol with enzymes sbf1 and msp1. Reads were demultiplexed in pyrad v 3-0-65 (command "pyrad -p params.txt -s 1") but have had no other processing.Pciris_reads_2of6100bp SR sequence reads from an illumina Hiseq 2500 for Passerina ciris. Library prepped via ddRAD protocol with enzymes sbf1 and msp1. Reads were demultiplexed in pyrad v 3-0-65 (command "pyrad -p params.txt -s 1") but have had no other processing.Pciris_reads_3of6100bp SR sequence reads from an illumina Hiseq 2500 for Passerina ciris. Library prepped via ddRAD protocol with enzymes sbf1 and msp1. Reads were demultiplexed in pyrad v 3-0-65 (command "pyrad -p params.txt -s 1") but have had no other processing.Pciris_reads_4of6100bp SR sequence reads from an illumina Hiseq 2500 for Passerina ciris. Library prepped via ddRAD protocol with enzymes sbf1 and msp1. Reads were demultiplexed in pyrad v 3-0-65 (command "pyrad -p params.txt -s 1") but have had no other processing.Pciris_reads_5of6100bp SR sequence reads from an illumina Hiseq 2500 for Passerina ciris. Library prepped via ddRAD protocol with enzymes sbf1 and msp1. Reads were demultiplexed in pyrad v 3-0-65 (command "pyrad -p params.txt -s 1") but have had no other processing.Pciris_reads_6of6100bp SR sequence reads from an illumina Hiseq 2500 for Passerina ciris. Library prepped via ddRAD protocol with enzymes sbf1 and msp1. Reads were demultiplexed in pyrad v 3-0-65 (command "pyrad -p params.txt -s 1") but have had no other processing.