DOI: 10.25345/c5nc5ss2w
title: MassIVE MSV000100564 - Degradation of deuterated NIs (DMPP and MBOA) in soil
title: Goal :&lt;br&gt; Detection of unknown degradation products of the nitrification inhibitors DMPP (SNI) and MBOA (BNI) in three different soils (Wieselburg, Marchfeld, and Linz).&lt;br&gt; &lt;br&gt; List of samples:&lt;br&gt; Same labeling scheme for all three soils (L, MF, WB)&lt;br&gt; Sample NR | Timepoint | Treatment | weighed soil [g] | Which medium is it stored in&lt;br&gt; 1 | T0 (same day) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 5 | T0 (same day) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 6 | T0 (same day) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 10 | T0 (same day) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 11 | T0 (same day) | MBOA | 0.25 | Acetonirile&lt;br&gt; 15 | T0 (same day) | MBOA | 0.25 | Acetonirile&lt;br&gt; 16 | T0 (same day) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 20 | T0 (same day) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 21 | T0 (same day) | Control | 0.25 | HPLC-eluent&lt;br&gt; 25 | T0 (same day) | Control | 0.25 | Acetonirile&lt;br&gt; 26 | T1 (after 3 days) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 30 | T1 (after 3 days) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 31 | T1 (after 3 days) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 35 | T1 (after 3 days) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 36 | T1 (after 3 days) | MBOA | 0.25 | Acetonirile&lt;br&gt; 40 | T1 (after 3 days) | MBOA | 0.25 | Acetonirile&lt;br&gt; 41 | T1 (after 3 days) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 45 | T1 (after 3 days) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 46 | T1 (after 3 days) | Control | 0.25 | Acetonirile&lt;br&gt; 50 | T1 (after 3 days) | Control | 0.25 | HPLC-eluent&lt;br&gt; 51 | T2 (after 10 days) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 55 | T2 (after 10 days) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 56 | T2 (after 10 days) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 60 | T2 (after 10 days) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 61 | T2 (after 10 days) | MBOA | 0.25 | Acetonirile&lt;br&gt; 65 | T2 (after 10 days) | MBOA | 0.25 | Acetonirile&lt;br&gt; 66 | T2 (after 10 days) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 70 | T2 (after 10 days) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 71 | T2 (after 10 days) | Control | 0.25 | HPLC-eluent&lt;br&gt; 75 | T2 (after 10 days) | Control | 0.25 | Acetonirile&lt;br&gt; 76 | T3 (after 14 days) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 80 | T3 (after 14 days) | DMPP | 0.25 | HPLC-eluent&lt;br&gt; 81 | T3 (after 14 days) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 85 | T3 (after 14 days) | DMPP - stable isotope | 0.25 | HPLC-eluent&lt;br&gt; 86 | T3 (after 14 days) | MBOA | 0.25 | Acetonirile&lt;br&gt; 90 | T3 (after 14 days) | MBOA | 0.25 | Acetonirile&lt;br&gt; 91 | T3 (after 14 days) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 95 | T3 (after 14 days) | MBOA - stable isotope | 0.25 | Acetonirile&lt;br&gt; 96 | T3 (after 14 days) | Control | 0.25 | Acetonirile&lt;br&gt; 100 | T3 (after 14 days) | Control | 0.25 | HPLC-eluent&lt;br&gt; &lt;br&gt; Concentrations:&lt;br&gt; all smaples have 130ug of (NH4)2SO4&lt;br&gt; DMPP samples have 1,3ug of DMPP per 1 g soil&lt;br&gt; MBOA samples have 300ug of MBOA per 1 g soil&lt;br&gt; for samples that are isotopically labeled the amount of Inhibitor was split between the unlabeled and labeled compound&lt;br&gt; 5ul of the respective solution was added to the sample.&lt;br&gt; &lt;br&gt; Measurements according to 10.1021/ac503290j, but with an Q Exactive HF instrument&lt;br&gt; &lt;br&gt; Results: &lt;br&gt; D patterns unfortunately also always show up in the T0 controls --&gt; so it's unlikely to be biological breakdown/degradation products&lt;br&gt; &lt;br&gt;