cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/fdcm0-q2241
title: M222R chemoreceptor mutant

description: M222R HAMP mutant locked in 'CHeA inactivating state'
description: <strong>Tilt Series Date:</strong> 2010-03-19</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> John Parkinson </p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB medium, induced with salycilate (0.6 µM) for three hours, then penicillin treated for one hour </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-03-19-20/rawdata/</p>10mar19a_EcoliM222K_020.st,  Tilt Series (Pixel Size 0.632 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-03-19-20/rawdata/10mar19a_EcoliM222K_020.st"
        > <i class="download icon"></i> Download </a></p>

        10mar19a_EcoliM222K_020.rec,  Reconstruction (Pixel Size 0.632 nm), 249.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-03-19-20/3dimage_12817/10mar19a_EcoliM222K_020.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wbpz4-4fg12
title: E. coli (pLE6, 20 um IPTG)

description: e. coli from Larry Rothfield containing plasmid carrying WT MreB.
description: <strong>Tilt Series Date:</strong> 2011-07-29</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Sample Preparation:</strong> Induced with 20um IPTG for 2 hours starting at OD600=0.2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-07-29-11/3dimage_44260/</p>20110729_ple6_20umIPTG0003_full.rec,  Reconstruction (Pixel Size 1.892 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-07-29-11/3dimage_44260/20110729_ple6_20umIPTG0003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20110729_ple6_20umIPTG0003.mrc,  Tilt Series (Pixel Size 0.946 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-07-29-11/rawdata/20110729_ple6_20umIPTG0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ff5mb-3w078
title: E.coli 16/13 mini cells

description: E.coli flagella motor searchKeywords: flagella, chemoreceptors
description: <strong>Tilt Series Date:</strong> 2008-04-22</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 300.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> FEI Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in standard media</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2008-04-22-3/rawdata/</p>Ecoli1613_Sm1_4.mrc,  Tilt Series (Pixel Size 0.64 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2008-04-22-3/rawdata/Ecoli1613_Sm1_4.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli1613_Sm1_4_1k.rec,  Reconstruction (Pixel Size 1.28 nm), 440.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2008-04-22-3/3dimage_2882/Ecoli1613_Sm1_4_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9zh2s-fcr35
title: MP II3 27.5KX

description: Mycoplasma pneumonia II3 mutant, ok, not a cell
description: <strong>Tilt Series Date:</strong> 2009-10-15</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Mycoplasma pneumoniae</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> data collected by Jian; processed by Jian</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-10-15-19/rawdata/</p>09oct15a_Box169C_021.mrc,  Tilt Series (Pixel Size 0.774 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-10-15-19/rawdata/09oct15a_Box169C_021.mrc"
        > <i class="download icon"></i> Download </a></p>

        09oct15a_Box169C_021_full.rec,  Reconstruction (Pixel Size 1.548 nm), 367.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-10-15-19/3dimage_16581/09oct15a_Box169C_021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4y1y6-fvk22
title: Agrobacterium tumefaciens

description: Agrobacterium_VirB8-GFP
description: <strong>Tilt Series Date:</strong> 2013-06-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from PatZambryski</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-02-2/rawdata/</p>agro_virb80002.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-02-2/rawdata/agro_virb80002.mrc"
        > <i class="download icon"></i> Download </a></p>

        2_full.rec,  Reconstruction (Pixel Size 1.92 nm), 791.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-02-2/3dimage_32296/2_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.87b3yr
title: Occurrence Download

description: A dataset containing 42 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Malva neglecta",
 "Country is South Africa",
 "HasCoordinate is true"
 ]
}

 The dataset includes 42 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044965-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/124bm-r0152
title: R. sphaeroides Cryosections

description: Strain JPA 543 cryosections
description: <strong>Tilt Series Date:</strong> 2013-07-26</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Mark Ladinsky, Judy Armitage</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-26-63/rawdata/</p>rhodobactertestb0069.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-26-63/rawdata/rhodobactertestb0069.mrc"
        > <i class="download icon"></i> Download </a></p>

        rhodobactertestb0069_full.rec,  Reconstruction (Pixel Size 0.52 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-26-63/3dimage_29053/rhodobactertestb0069_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cs414-40s40
title: R. sphaeroides Cryosections

description: R. sphaeroides Cryosections strain 593
description: <strong>Tilt Series Date:</strong> 2013-10-23</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 44000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judith Armitage</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-23-62/rawdata/</p>rhodosectionsatlas20009.mrc,  Tilt Series (Pixel Size 0.32 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-23-62/rawdata/rhodosectionsatlas20009.mrc"
        > <i class="download icon"></i> Download </a></p>

        rhodosectionsatlas20009_full.rec,  Reconstruction (Pixel Size 0.64 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-23-62/3dimage_33159/rhodosectionsatlas20009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7hyr7-f0e83
title: Agrobacterium tumefaciens

description: Agrobacterium_VirB8-GFP_deltaVirB8
description: <strong>Tilt Series Date:</strong> 2013-06-14</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from PatZambryski</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-14-21/rawdata/</p>virb8-delta-agar0008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-14-21/rawdata/virb8-delta-agar0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        virb8-delta-agar0008_part121_4.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-14-21/3dimage_32557/virb8-delta-agar0008_part121_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qxmrs-ve872
title: R. sphaeroides Cryosections

description: R. sphaeroides Cryosections strain 593
description: <strong>Tilt Series Date:</strong> 2013-10-23</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 44000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judith Armitage</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-23-11/rawdata/</p>rhodosectionsatlas10013.mrc,  Tilt Series (Pixel Size 0.32 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-23-11/rawdata/rhodosectionsatlas10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        rhodosectionsatlas10013_full.rec,  Reconstruction (Pixel Size 0.64 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-23-11/3dimage_33058/rhodosectionsatlas10013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kzds5-ezm10
title: dK203 uninduced grown 2 days

description: dK203 is the dQ167 strain in which dK has also been deactivated. It is only inducible for dQ.
description: <strong>Tilt Series Date:</strong> 2014-12-21</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum </p>
<strong>Strain:</strong> deltaQ 167</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Arash Komeili</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-12-21-24/rawdata/</p>dkuninduced_atlas6_montage20005.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-12-21-24/rawdata/dkuninduced_atlas6_montage20005.mrc"
        > <i class="download icon"></i> Download </a></p>

        dkuninduced_atlas6_montage20005_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-12-21-24/3dimage_49906/dkuninduced_atlas6_montage20005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kj9sm-12c13
title: M259Q

description: E. coli mutant locked in the CheA activating state. Penicillin treated
description: <strong>Tilt Series Date:</strong> 2010-11-23</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: °, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -25.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, CTF corrected</p>
<strong>Collaborators and Roles:</strong> John Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB medium (30 degrees), lysed with penicillin 1 hour</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-11-23-4/rawdata/</p>10nov23c_M259Q_002.st,  Tilt Series (Pixel Size 0.0 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-11-23-4/rawdata/10nov23c_M259Q_002.st"
        > <i class="download icon"></i> Download </a></p>

        10nov23c_M259Q_002.rec,  Reconstruction (Pixel Size 0.0 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-11-23-4/3dimage_14987/10nov23c_M259Q_002.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.whw6qq
title: Occurrence Download

description: A dataset containing 9848 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Malva neglecta",
 "Country is United Kingdom of Great Britain and Northern Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 9848 records from 62 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044969-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/319f9-zeq44
title: C65_HuvecspNL24h_11122012

description: Whole cells were grown on Au finder grids and infected with pNLGFP. Sample frozen 24h post infection
description: <strong>Tilt Series Date:</strong> 2011-11-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-11-12-27/rawdata/</p>12nov10_C65_HuvecspNL24h_AT1BT2_0027.mrc,  Tilt Series (Pixel Size 0.45 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-11-12-27/rawdata/12nov10_C65_HuvecspNL24h_AT1BT2_0027.mrc"
        > <i class="download icon"></i> Download </a></p>

        12nov10_C65_HuvecspNL24h_AT1BT2_0027_full.rec,  Reconstruction (Pixel Size 1.8 nm), 530.0 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.xhpmyc
title: Occurrence Download

description: A dataset containing 162 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Characidium etheostoma)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 162 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044968-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/g0f6b-5bb79
title: Tsr oe vesicles:W:A 1.5:0.5:1

description: Tsr overexpression vesicles with 1.5:0.5:1 Tst:W:A incubation for ternary complex formation
description: <strong>Tilt Series Date:</strong> 2013-03-14</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Kiessling, Heather Hodges</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Buffer Tsr reaction buffer (50 mM Tris-HCl pH 7.4 50mM KCl 5mM MgCl2 0.5 Mm TCEP</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-14-8/3dimage_25556/</p>HLH3_031313_16_full.rec,  Reconstruction (Pixel Size 1.22 nm), 838.9 MB  
        
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        HLH3_031313_16.mrc,  Tilt Series (Pixel Size 0.61 nm), 562.0 MB  
        
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