cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/a5xtk-9m294
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 10 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-01-14</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-32/rawdata/</p>stiv10h40022.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-32/rawdata/stiv10h40022.mrc"
        > <i class="download icon"></i> Download </a></p>

        stiv10h40022_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-32/3dimage_37728/stiv10h40022_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/avarh-3ff16
title: Myxococcus xanthus DK1622 wt

description: Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-03-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> FEIBatchTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-03-27-3/rawdata/</p>DK1622_UCLA_20130327_12.mrc,  Tilt Series (Pixel Size 1.352 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-03-27-3/rawdata/DK1622_UCLA_20130327_12.mrc"
        > <i class="download icon"></i> Download </a></p>

        DK1622_UCLA_20130327_12_part131_7.rec,  Reconstruction (Pixel Size 2.704 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-03-27-3/3dimage_26037/DK1622_UCLA_20130327_12_part131_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c8m8q-rc536
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-09-30</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-68.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-8/rawdata/</p>RCC745_G1tovis_135.mrc,  Tilt Series (Pixel Size 1.26 nm), 746.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-8/rawdata/RCC745_G1tovis_135.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_135.rec,  Reconstruction (Pixel Size 1.26 nm), 149.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-8/3dimage_12103/RCC745_G1tovis_135.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/466vv-4xs47
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-11/rawdata/</p>RCC745_RELtovis_011.mrc,  Tilt Series (Pixel Size 1.26 nm), 771.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-11/rawdata/RCC745_RELtovis_011.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_011.rec,  Reconstruction (Pixel Size 1.26 nm), 267.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-11/3dimage_12363/RCC745_RELtovis_011.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.yppfpk
title: Occurrence Download

description: A dataset containing 42 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea chamaedryfolia",
 "Country is Czechia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 42 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002602-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/mnm2b-1b370
title: Sulfolobus cells synchronized for division

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2011-01-14</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 1.5 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 5 sec, sponge for humidity</p>
  mdj2011-01-14-4_slicer8574.jpg: pili .  Sulfolobus acidocaldarius mdj2011-01-14-4_slicer8575.jpg: filament .  Sulfolobus acidocaldarius mdj2011-01-14-4_slicer8576.jpg: filament .  Sulfolobus acidocaldarius mdj2011-01-14-4_slicer8577.jpg: filament .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-14-4/rawdata/</p>11jan14a_Bx8g3_006.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-14-4/rawdata/11jan14a_Bx8g3_006.mrc"
        > <i class="download icon"></i> Download </a></p>

        11jan14a_Bx8g3_006_full.rec,  Reconstruction (Pixel Size 2.5 nm), 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-14-4/3dimage_19182/11jan14a_Bx8g3_006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pbhv3-15q63
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-12-10</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-12-10-38/rawdata/</p>RCC745_G1tovis_343.mrc,  Tilt Series (Pixel Size 0.96 nm), 671.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-12-10-38/rawdata/RCC745_G1tovis_343.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_343.rec,  Reconstruction (Pixel Size 0.96 nm), 896.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-12-10-38/3dimage_12327/RCC745_G1tovis_343.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n6wkh-22619
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-11-16</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-32/rawdata/</p>RCC745_MGtovis_433.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-32/rawdata/RCC745_MGtovis_433.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_433.rec,  Reconstruction (Pixel Size 1.26 nm), 295.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-32/3dimage_11829/RCC745_MGtovis_433.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sbak5-9n325
title: Pfl 121509 MP36

description: Prosthecobacter fluviatilis, 10jan14a_MP36_Pfl-stat_002, classII tube,
description: <strong>Tilt Series Date:</strong> 2010-01-14</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Prosthecobacter fluviatilis</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
  mpa2010-01-14-1_slicer4190.jpg: Protein-decorated innermembrane vesicle mpa2010-01-14-1_slicer4192.jpg: Horseshoe vesicle mpa2010-01-14-1_slicer4194.jpg: Intracellular vesicle mpa2010-01-14-1_slicer4198.jpg: Tube mpa2010-01-14-1_slicer9025.jpg: horseshoe .  Prosthecobacter .  snapshot by Megan
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-01-14-1/rawdata/</p>10jan14a_MP36_Pfl-stat_002.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-01-14-1/rawdata/10jan14a_MP36_Pfl-stat_002.mrc"
        > <i class="download icon"></i> Download </a></p>

        10jan14a_MP36_Pfl-stat_002_part.rec,  Reconstruction (Pixel Size 1.9 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-01-14-1/3dimage_14419/10jan14a_MP36_Pfl-stat_002_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d63th-rry36
title: HeLa siRNA tfx CHMP2A/B infx pNLGFP

description: HeLa cells infected with pNLGFP and 12h post infection cells were transfected with siRNA targeting CHMP2A and CHMP2B. 18h after siRNA tfx, cells were frozen Sample# C85
description: <strong>Tilt Series Date:</strong> 2011-06-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP2A and 2B siRNAs provided by Wes Sundquist. pNLGFP proviral construct provided by Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward.  Cells transfected, infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and infected and siRNA transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-28-3/rawdata/</p>11jun28b_atlas1_359_0003_new.st,  Tilt Series (Pixel Size 0.5 nm), 867.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-28-3/rawdata/11jun28b_atlas1_359_0003_new.st"
        > <i class="download icon"></i> Download </a></p>

        11jun28b_atlas1_359_0003_new_full.rec,  Reconstruction (Pixel Size 2.0 nm), 607.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-28-3/3dimage_68393/11jun28b_atlas1_359_0003_new_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rnq98-p2v38
title: Myxococcus xanthus pilM-YFP

description: strain SA4088grid box YWC101
description: <strong>Tilt Series Date:</strong> 2014-05-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-05-14-7_slicer5158.jpg: Filament .  Pilli
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-14-7/rawdata/</p>20140514_Janelia_YWC096_10007.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-14-7/rawdata/20140514_Janelia_YWC096_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140514_Janelia_YWC096_10007_part131_9.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-14-7/3dimage_42610/20140514_Janelia_YWC096_10007_part131_9.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s6jr5-3gn33
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-93/rawdata/</p>RCC745_RELtovis_102.mrc,  Tilt Series (Pixel Size 1.26 nm), 570.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-93/rawdata/RCC745_RELtovis_102.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_102.rec,  Reconstruction (Pixel Size 1.26 nm), 762.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-93/3dimage_12527/RCC745_RELtovis_102.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eqg1b-p6247
title: E. coli (pLE7, 20 uM IPTG)

description: Cells contain WT MreB in the chromosome plus an N-terminal YFP fusion to MreB (YFP-MreB) on the plasmid pLe7. pLE7 also confers a resistance to ampicillin.
description: <strong>Tilt Series Date:</strong> 2011-11-05</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Gift from Larry Rothfield</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot plunge freeze.</p>
  mst2011-11-05-14_slicer9449.jpg: Escherichia coli .  filament mst2011-11-05-14_slicer9450.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-11-05-14/rawdata/</p>20111105_ple7_20uM0002.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-11-05-14/rawdata/20111105_ple7_20uM0002.mrc"
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        20111105_ple7_20uM0002_full.rec,  Reconstruction (Pixel Size 1.9 nm), 825.8 MB  
        
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