cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14306430.v1
title: Effect of okara levels on corn grain silage

description: ABSTRACT We ensiled different levels of okara and ground corn to evaluate the effects on the fermentative pattern, aerobic stability, and chemical composition of resulting silages. The experimental design was completely randomized with four replicates per treatment. The okara levels were (dry matter basis): control (without okara) and 200, 300, 400, and 500 g kg−1 okara, with four replicates per treatment. Control silage did not contain okara, but water was added to adjust the moisture content (400 g kg−1 as fed). Mixtures were ensiled in lab-scale silos and stored for 150 days. Compared with the control silage, okara inclusion linearly increased crude protein (from 89.1 to 251 g kg−1 DM), ether extract (from 39.6 to 136 g kg−1 DM), neutral detergent fiber (from 79.9 to 174 g kg−1 DM), acid detergent fiber (from 22.4 to 119 g kg−1 DM), and ash (from 12.2 to 32.4 g kg−1 DM), whereas decreased dry matter content and in vitro dry matter digestibility (from 830 to 730 g kg−1 DM). The use of okara linearly increased lactic acid concentration but also intensified secondary fermentation. On the other hand, aerobic stability of silages increased due to okara inclusion because of the higher amount of short-chain fatty acids, such as butyric and acetic acids, which accumulated during fermentation. Okara inclusion in corn grain silage must be conditioned to the dry matter content at ensiling, but must not exceed 200 g kg−1 on dry matter basis.
DOI: 10.6084/m9.figshare.14306429.v1
title: Developmental expression patterns and correlation analysis of TLR4 and its downstream genes in the intestinal and immune tissues of Meishan pigs

description: ABSTRACT To understand the developmental expression patterns of key genes in the Toll-like receptor 4 ( TLR4 ) pathway and their regulatory characteristics in the immune response in pigs, we examined TLR4 and its downstream genes expression levels in the intestinal and immune tissues of Meishan pigs. The genes were expressed in all examined tissues at the different developmental stages. TLR4 expression was higher in spleen and lower in other tissues. Spleen and lymph TLR4 expression was significantly lower in 7- and 35-day-old pigs; in the intestinal tissues, it was significantly lower in 21- and 35-day-old pigs. IFNA, IL1B , and TNFA expression varied greatly with developmental stage; expression was significantly higher in most tissues in 21-, 134-, and 158-day-old pigs. TLR4 was highly positively correlated with TNFA in the immune tissues and was significantly correlated with all downstream genes in the spleen; there was no significant correlation in the intestinal tissues. There was near significant positive correlation among the downstream genes in the intestinal tissues, but almost no significant correlation in the immune tissues. We speculated that the TLR4 pathway genes may have an anti-lipopolysaccharide invasion effect during weaning, and the high expression of the downstream genes is beneficial for improving immunity in adult pigs. Our results may contribute to better understanding the TLR4 signaling pathway and its molecular mechanisms and could provide a reference and basis for appropriate-age experimental animal selection for relevant future research.
DOI: 10.6084/m9.figshare.14306427.v1
title: Identification of novel single nucleotide polymorphisms in the growth hormone ( GH ) gene in Anatolian water buffalo ( Bubalus bubalis ) populations in Turkey

description: ABSTRACT This study was conducted to investigate the growth hormone ( GH; somatotropin-like) gene polymorphisms in 150 water buffalo ( Bubalus bubalis ) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3’ UTR regions of the GH gene (also called GH/Alu I locus) and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene (also called GH/Msp I locus) were amplified, and their PCR products analyzed via DNA sequencing method. Seven genotypes due to twenty single nucleotide polymorphisms (SNP) and one deletion/insertion were identified in a 347 bp long region of the GH/Msp I locus. A missense mutation from glycine to glutamate amino acid and four silent mutations in the serine, threonine, and asparagine amino acids were determined in the exon 3 region of the GH gene. Four genotypes due to eight SNP were identified in a 404 bp long region of the GH/Alu I locus. A missense mutation from lysine to arginine amino acid and six silent mutations in Leucine, aspartate, histidine, lysine, arginine, and cysteine amino acids were revealed in the exon 5 region of the GH gene. The partial DNA sequence of the GH gene in water buffalos was reported, and these sequences were deposited at the NCBI Genbank database with the accession numbers MN266903-MN266909 and MN530973-MN530976. These SNP may have an effect on economic (such as body composition) and carcass traits, reproduction, and milk yield and content in water buffalo populations and may prove to be useful for water buffalo breeding.
DOI: 10.6084/m9.figshare.14306424.v1
title: Markers and antibodies for characterization of goat mammary tissue and the derived primary epithelial cell cultures

description: ABSTRACT A selection of commercially available antibodies, targeted against markers employed in studies of mammary gland biology, was tested to determine their reactivity in goat mammary tissue and the derived tissue cultures. Expression of the markers smooth muscle actin ( SMA ), selected keratins ( KRT ) 5, 14, 18 , and 19 , CD24 molecule ( CD24 ), epithelial cell adhesion molecule ( EPCAM ), mucin 1 ( MUC1 ), integrin subunit alpha 6 ( ITGA6; CD49F ), integrin subunit beta 1 ( ITGB1; CD29 ), cyclin dependent kinase inhibitor 1A ( CDKN1A; p21 ), membrane metalloendopeptidase ( MME; CD10 ), progesterone receptor ( PGR ), estrogen receptor 1 ( ESR1 ), and vimentin ( VIM ) was first assessed on mRNA level, using reverse transcription PCR (RT-PCR). The reactivity of the antibodies in the tissue sections and the derived tissue cultures was determined using immunofluorescence. The result of this study is a list of commercially available antibodies, raised mostly against human antigens, which also recognize orthologous goat antigens and are useful for characterization of different mammary cell types. Additionally, primers that are functional in detecting expression of mammary lineage markers in goat mammary mRNA isolates were validated. The suggested antibodies, PCR primers, and the described methods are of practical value for researchers interested in characterization and isolation of cell types comprising mammary tissue of goats and probably other ruminants.
DOI: 10.6084/m9.figshare.14306421.v1
title: Apparent digestibility coefficients for amino acids of feed ingredients in tambaqui (Colossoma macropomum) diets

description: ABSTRACT This study evaluated the apparent digestibility coefficients (ADC) of essential (EAA) and non-essential (NEAA) amino acids of 13 ingredients for tambaqui (Colossoma macropomum) diets. Proteic and energetic ingredients were analyzed separately. The trial with energetic and proteic ingredients were arranged in a randomized block design, with four replicates: energetic ingredients (corn, wheat bran, broken rice, and sorghum) with four treatments, whereas proteic ingredients (corn gluten meal, soybean meal, poultry byproduct meal, salmon meal, fish meal [tilapia processing residue], wheat gluten meal, feather meal, cottonseed meal, and alcohol yeast [spray dried]) with nine treatments. Each block was considered as one round of fecal collection. A total of 420 tambaqui juveniles (mean initial weight: 70±8.58 g) were used. Among energetic ingredients, corn (94.6%) and wheat bran (91.9%) had the highest ADCEAA, followed by broken rice (75.7%), and sorghum (72.8%). On average, ADCEAA and ADCNEAA values of proteic ingredients were 79.5-98.5%, except for alcohol yeast (ADCEAA: 68.4 and ADCNEAA: 76.7%). Tryptophan was the first limiting amino acid in most ingredients tested and had the lowest chemical scores (0.06-0.51), except for wheat bran, corn gluten meal, and soybean meal, in which lysine was the first limiting amino acid. Soybean meal had the highest digestible essential amino acid index (EAAI: 1.02) and the most balanced amino acid profile, whereas wheat gluten meal had the lowest EAAI (0.48). Overall, tambaqui was very efficient to digest proteic and energetic ingredients.
DOI: 10.6084/m9.figshare.14306419.v1
title: Animal performance and welfare of giant freshwater prawn (Macrobrachium rosenbergii) subjected to feed restriction

description: ABSTRACT Behavior activities of giant freshwater prawn Macrobrachium rosenbergii juvenile, their performance, and acquired lesions when under temporary feed restrictions were analyzed. Five animals/experimental units (10 prawns/m2) were employed for treatments DFL – daily feed supply in the light phase; DFD – daily feed supply in the dark phase; AFL – alternating feed supply in the light phase; and AFD – alternating feed supply in the dark phase, for a 24-hour period, with four replications each. Prawns were fed a pellet diet, twice a day, with 10% of their biomass/day. Specimens were monitored during 60 consecutive days, with behavior recording daily and weighed every 10 days, with assessment of lesions and/or amputated limbs at the end of the experimental period. Types of behavior comprised exploring, grooming, inactivity, crawling, swimming, excavation, burrowing, agonism, and feed ingestion by scan sampling, with instantaneous registration every 60 s, in 15-min windows, before and after feed, in light and dark phases. Feed restricted in alternate days during light and dark phases did not interfere in growth or survival of the specimens, with increase in apparent feed intake and less feed conversion ratio and specific growth rate. Agonistic behavior was higher in specimens with feed restrictions, with an increase in the number of lesions and amputations. The above demonstrates welfare decrease in farmed M. rosenbergii and depreciation in the quality of the final product.
DOI: 10.6084/m9.figshare.14306417.v1
title: Slaughter body weights of Jersey young bulls finished on feedlot

description: ABSTRACT The objective of this study was to investigate the effects of slaughter body weight (SW) on the performance, carcass characteristics, and meat quality of Jersey bulls in feedlots from weaning to slaughter. Eighteen Jersey bulls (125.7±15.9 kg; 5±0.6 months old) were used in the study. The treatments corresponded to three slaughter-weights: L360 – light weight, SW of 360 kg, M390 – medium weight, SW of 390 kg, and H420 – heavy weight, SW of 420 kg (n = 6/treatment); the animals were slaughtered at 368, 392, and 422 kg, respectively. The average daily gain was not influenced by SW. The M390 and H420 groups remained in feedlot, on average, 6 and 51 d longer, respectively, to reach a similar SW in relation to the L360 group. The increase in SW did not influence dry matter intake. However, the L360 group exhibited greater dry matter intake relative to body weight compared with the other two groups (29 vs. 26 g/kg). The increase in SW resulted in heavier and longer carcasses, with higher hot carcass yields. However, animals with greater SW had lower fat thickness. In addition, the increase in SW led to greater pistol cut, bone, and muscle weight for the carcasses. Although the meat color of Jersey bulls was darker in the H420 group, the meat was considered softer and more palatable by the consumer panel compared with the meat from the L360 group. The L360 group exhibited less fluid loss during thawing and cooking. Shear force measured by Warner Blatzer Shear was lower for H420. Animals slaughtered in the L390 group had an intermediate carcass weight, similar carcass yield, better cover fat and meat quality (color and shear) than animals slaughtered in the H420. Combining these factors with a shorter feedlot time compared with that of heavier animals, the SW of feedlot Jersey bulls is recommended at 390 kg.
DOI: 10.6084/m9.figshare.14306415.v1
title: Public policies for enhancing diffusion of technology: a network analysis for a dairy farmer community in Minas Gerais, Brazil

description: ABSTRACT The objective of this study is to apply Social Network Analysis (SNA) approach to improve the understanding of how to shape effective information diffusion policies among small-scale dairy farmers in rural communities of Brazil, for whom milk production is an important source of income. We interviewed 24 dairy farmers representing all participants of a dairy farmer association in a small-scale production community in the Zona da Mata of Minas Gerais, Brazil. Two sociograms were built by means of an adjacency matrix representing the two internal networks, friendship (F) and advising (A). The beta-centrality and betweenness centrality measures were calculated to identify central farmers and correlate with efficiency indicators in each network (F and A) using a quadratic assignment procedure (QAP). The two internal networks structures and individual thresholds of adoption were represented in an agent-based model (ABM) to simulate the spread of the information among farmers. Our simulations indicate that the assortativity degree of social networks affects the information diffusion among farmers, highlighting the importance of opinion leaders versus convincing a critical mass to implement innovation strategies. Our analysis suggests that vertical policies focused on local opinion leaders will be more effective for enhancing the adoption of techniques that involve investments in private goods such as the adoption of artificial insemination, whereas horizontal policies, such as broadcasting information, will be more effective in encouraging investments in public and semi-public goods, such as the purchase of community cooling tanks and techniques for enhancing milk quality.
DOI: 10.6084/m9.figshare.14306412.v1
title: Liver histology and hematological parameters of female Rhamdia quelen fed different lipid sources

description: ABSTRACT The objective with this study was to evaluate the liver histology and hematological parameters of female Rhamdia quelen fed diets supplemented with different oils: 5% marine fish oil, 5% refined palm oil, 5% soybean oil, and a combination of the three. The lipid vacuolization (steatosis) in the liver was analyzed according to a score of vacuolization when: 0 = absence, 1 = reduced, 2 = intermediate, and 3 = intense. At the end of the experiment, females (n = 10) were selected from each treatment (two per cage), and the blood was collected for erythrocyte and biochemical analysis. Lower vacuolization indices in the liver were observed at the beginning of the experiment. However, with time, the presence of vacuoles was more evident but presented similar morphology among all treatments. The blood parameters were also not influenced by the different diets, except for glucose levels, which was higher in the treatment with the mixture of oils. The absence of differences regarding liver morphology (steatosis) and hematological parameters indicates that the replacement of fish oil by vegetable oils could be performed without damage to the health of R. quelen females.
DOI: 10.6084/m9.figshare.14306411.v1
title: Identifying parents and generating hybrids with high combining ability for yielding fresh cocoon and raw silk in silkworm (Bombxy mori L.)

description: ABSTRACT This study was carried out to identify parents and hybrids by determination of general combining ability (GCA), specific combining ability (SCA), and heterosis in pure lines and their hybrids, which were selected from our gene sources, to determine new hybrid combinations that could be alternative or higher in yielding compared with M × N hybrid used in production. Combining ability and heterosis effects were studied for the eight quantitative characters through line × tester mating design. Chinese origin lines (KZ, ZF) were identified as females and Japanese origin lines (BR, ZB) as males, and by these, four hybrids were derived. M, N, and their hybrid were both used as control genotypes and to calculate the heterosis and commercial heterosis as well. All the genotypes were reared in standard conditions, in three replications. ZF (for pupa survival rate) and ZB (for hatching and filament length) presented significantly higher and positive GCA effects. In addition, ZB had negative GCA effect for infertile egg rate. Effects of SCA were not significant for all the characters. In conclusion, ZF × ZB hybrid could have a potential for the higher cocoon and silk production in Turkey.
DOI: 10.6084/m9.figshare.14306408.v1
title: Determination of optimum agricultural policy for buffalo breeding

description: ABSTRACT The present study aimed to determine the optimum agricultural policy components for buffalo breeding respecting the welfare of breeders. The data used for the study was composed of microdata retrieved from buffalo breeders in Turkish provinces, where buffalo breeding is widespread. The data retrieved via face to face survey was analyzed and interpreted with descriptive statistics and Conjoint Analysis, which is one of the multivariate analysis techniques. In this study, we investigated and discussed the scope of supporting policies provided to buffalo breeders to promote buffalo breeding and to assure its sustainability. Besides, this research is a preliminary and innovative study that intended to focus on breeders' utility in the evaluation of supports provided to buffalo breeding. According to the findings, the most critical factor for buffalo breeders in terms of producer-oriented policy framework appeared as “subsidy in cash paid per head” (32.10%), which was followed by “investment support” with 21.25%. In addition to these tools, “marketing support” (16.51%), “product support” (16.18%), and “investment credit” (13.96%) appeared as essential and vital policy tools.
DOI: 10.6084/m9.figshare.14306405.v1
title: Producers' knowledge and perception about environmental enrichment and materials used in pig farms

description: ABSTRACT This study investigated the knowledge and perception of Brazilian pig producers in terms of environmental enrichment (EE) for pigs, the materials used, and the forms of presentation, identifying the single conditions that can improve their application on pig farms. A questionnaire was applied to 1340 farms – representing 7.4% of the farms and 12% of the sows in the country. The questions included descriptions of farms and respondents, their knowledge, judgments and level of interest in the use of EE, and frequency of use of the materials. Enrichment was used in 89.1% of farms, but half of the respondents admitted to using it without knowing what was involved. The producers presented an optimistic view (92.3%) about the application of this tool and were interested in receiving more knowledge and guidance on the subject (97.8%). The materials were used mainly to avoid fights (46.3%) and to prevent tail biting (23.3%); on the other hand, the increase in production costs (39.6%) and lack of knowledge about the subject (31.3%) were the main reasons for not using enrichment; concerns about increased management on farms were sporadic (7.1%). Metal chains were the main artifacts used, followed by plastic containers and pieces of wood; the same breeding farm used up to five types of materials. The fact that the respondents stated that they knew what EE was and that they had a good perspective on its use were significant conditions for the use of some kind of enrichment on the farms. Younger farm owners were more likely to use enrichment materials than more experienced ones. The results suggested that Brazilian pig producers use EE even with limited knowledge about the subject and that there is an argument to improve the use of this animal welfare strategy.
DOI: 10.6084/m9.figshare.14306404.v1
title: The risk of post-polypectomy bleeding among patients receiving antithrombotic agents: A prospective observational study

description: ABSTRACT BACKGROUND: In July 2012, the Japan Gastroenterological Endoscopy Society updated their guidelines for gastroenterological endoscopy in patients receiving antithrombotic therapy. Colonoscopic polypectomy procedures are associated with a high risk of bleeding. OBJECTIVES: The present study evaluated the safety of colonoscopic polypectomy procedures in terms of bleeding, among patients receiving antithrombotic therapy. DESIGN AND SETTING: Prospective observational study conducted in a tertiary-level public cardiovascular hospital in Istanbul, Turkey. METHODS: Colonoscopic polypectomies carried out in a single endoscopy unit between July 2018 and July 2019 were evaluated prospectively. The patients’ data, including age, gender, comorbidities, whether antithrombotic drug use was ceased or whether patients were switched to bridging therapy, polyp size, polyp type, polyp location, histopathology, resection methods (hot snare, cold snare or forceps) and complications relating to the procedures were recorded. RESULTS: The study was completed with 94 patients who underwent a total of 167 polypectomy procedures. As per the advice of the physicians who prescribed antithrombotic medications, 108 polypectomy procedures were performed on 60 patients without discontinuing medication and 59 polypectomy procedures were performed on 34 patients after discontinuing medication. The age, gender distribution and rate of bleeding did not differ significantly between the patients whose medication was discontinued and those whose medication was continued (P &gt; 0.05). CONCLUSION: This study found that the colonoscopic polypectomy procedure without discontinuation of antithrombotic medication did not increase the risk of bleeding. This procedure can be safely performed by experienced endoscopists in patients with an international normalized ratio (INR) below 2.5.
DOI: 10.6084/m9.figshare.14306403.v1
title: Association between frailty syndrome and sedentary behavior among community-dwelling older adults in the Amazon region: a cross-sectional study

description: ABSTRACT BACKGROUND: Existence of an association between sedentary behavior and frailty among older adults has been suggested. However, there is a lack of studies conducted in Brazil, especially in areas of the Amazon region. OBJECTIVE: To analyze the association between frailty syndrome and sedentary behavior among community-dwelling older adults. DESIGN AND SETTING: Cross-sectional study carried out in Macapá, state of Amapá, Brazil. METHODS: Frailty status was assessed using Fried's frailty phenotype, and sedentary behavior was evaluated using two questions concerning time spent in a seated position, from the International Physical Activity Questionnaire (IPAQ). A multinomial logistic regression model was used to verify the association between frailty syndrome and sedentary behavior. RESULTS: The final study sample was made up of 411 older adults with a mean age of 70.14 ± 7.25 years and an average daily duration of sedentary behavior of 2.86 ± 2.53 hours. The prevalence of non-frailty was 28.7%, prevalence of pre-frailty was 58.4% and prevalence of frailty was 12.9%. The adjusted analysis showed that there were independent associations between sedentary behavior and pre-frailty (odds ratio, OR = 1.18; 95% confidence interval, CI: 1.03-1.34) and between sedentary behavior and frailty (OR = 1.20; 95% CI: 1.02-1.40). CONCLUSION: Frailty and pre-frailty status were associated with sedentary behavior among community-dwelling older adults.