cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7482518
title: Effect of adding fatty acids and surfactant on the functional properties of biodegradable films prepared with myofibrillar proteins from acoupa weakfish (Cynoscion acoupa )

description: Abstract This research aimed to assess the properties of biodegradable films prepared with lyophilized myofibrillar proteins (LMP) from fish filleting residues, fatty acids (stearic, palmitic, and caproic), and surfactant (SLS). The films were characterized to assess the effects of adding those components. Adding fatty acids and SLS resulted in more flexible films with higher elongation values compared to the LMP film. The films prepared with 5% stearic acid and 10% SLS and with 10% palmitic acid and 20% SLS had higher tensile strength compared to the LMP film. Solubility reached 100% in the films added with 10% fatty acids with and without SLS. Increasing the concentration of fatty acids and SLS led to less transparent films. Microscopy analysis showed changes in the morphological structure of the films added with fatty acids and SLS, resulting in whitish films when greater SLS contents were used.
DOI: 10.6084/m9.figshare.7482518.v1
title: Effect of adding fatty acids and surfactant on the functional properties of biodegradable films prepared with myofibrillar proteins from acoupa weakfish (Cynoscion acoupa )

description: Abstract This research aimed to assess the properties of biodegradable films prepared with lyophilized myofibrillar proteins (LMP) from fish filleting residues, fatty acids (stearic, palmitic, and caproic), and surfactant (SLS). The films were characterized to assess the effects of adding those components. Adding fatty acids and SLS resulted in more flexible films with higher elongation values compared to the LMP film. The films prepared with 5% stearic acid and 10% SLS and with 10% palmitic acid and 20% SLS had higher tensile strength compared to the LMP film. Solubility reached 100% in the films added with 10% fatty acids with and without SLS. Increasing the concentration of fatty acids and SLS led to less transparent films. Microscopy analysis showed changes in the morphological structure of the films added with fatty acids and SLS, resulting in whitish films when greater SLS contents were used.
DOI: 10.6084/m9.figshare.7482521.v1
title: Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion

description: Abstract Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules. Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran Defense Peptide. Furthermore, we performed caseinolytic activity test to assess the presence of serine and metalloproteases in skin secretion and it was fractionated by fast liquid protein chromatography using a reverse-phase column. The fractions were partially sequenced by Edman's degradation. Results: We were able to identify several classes of antimicrobial peptides, such as buforins, peroniins and brevinins, as well as PLA2, lectins and galectins, combining protein sequencing and RNA-seq analysis for the first time. In addition, we could isolate a PLA2 from the skin secretion and infer the presence of serine proteases in cutaneous secretion. Conclusions: We identified novel toxins and proteins from R. schneideri mucous glands. Besides, this is a pioneer study that presented the in depth characterization of protein molecules richness from this toad secretion. The results obtained herein showed evidence of novel AMP and enzymes that need to be further explored.
DOI: 10.7284/102944
title: Gyrocompass data collected during research cruise MGL1115 using a Kongsberg Simrad GC80 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7482506
title: Growth Performance, Carcass Traits and Breast Meat Fatty Acids Profile of Helmeted Guinea Fowls (Numida meleagris) Fed Increasing Level of Linseed (Linum usitatissimum) and Pumpkin Seed (Cucurbita moschata) Meals

description: ABSTRACT This study was conducted to determine the effects of dietary inclusion with linseed and pumpkin seed meals on growth performance, carcass traits and breast meat fatty acids profile of helmeted guinea fowls. A total of 120 meat-type helmeted guinea fowl females of 12 weeks of age were fed with 0 (T0), 100 (T1) and 200 (T2) g/kg of linseed (LM) and pumpkin seed meals (PSM) for 56 days, in a completely randomized design with 10 replicates per treatment and four birds per replicate. The inclusion up to 200 g/kg of LM and PSM did not affect the livability, initial live weight and feed intake (p&gt;0.05). However, final live weight and feed:gain ratio improved significantly (p&lt;0.05). The T1 increased (p&lt;0.05) the carcass yield and the T2 improved the breast weight and weight and yield of leg. Also, these experimental diets did not affect the carcass weight, breast yield and sensory quality of meat (p&gt;0.05). The oleaginous seeds (LM and PSM) decreased (p&lt;0.05) the mystic, palmitic and octadecanoic acids and the w-6/w-3 ratio, as well as increased the linoleic, a-linolenic, eicosapentanoic and docosahexanoic acids (p&lt;0.05), but did not modify the concentration of monounsaturated fatty acids (p&gt;0.05) and the eicosatrienoic and arachidonic acids in breast meat (p&lt;0.05). It is recommended the inclusion of 100 g/kg of LM and PSM to improve the live weight, weight and yield of the edible portions and the essential fatty acids in breast meat of guinea fowl, without affecting the sensory quality of the meat.
DOI: 10.6084/m9.figshare.7482512
title: Biofilme production by Staphylococcus chromogenes isolated from milk samples from bovine with mastitis

description: ABSTRACT A total of 135 crossbred cows was studied, from ten dairy herds on the state of Acre. The purpose was to identify species of Staphylococcus isolated from the mammary quarters of cows with mastitis. Additionally, the capacity of biofilm production by the species Staphylococcus chromogenes was evaluated. The sample characterization of the isolates was performed using the MALDI TOF MS (Matrix Associated Laser Desorption-Ionization - Time of Flight - Mass Spectrometry). The following species were identified by MALDI TOFF MS: S. chromogenes (36), Staphylococcus saprophyticus (5), S. chromogenes or Staphylococcus hycus (5), Staphylococcus haemolyticus (4), Staphylococcus epidermidis (3), Staphylococcus hycus (3), Staphylococcus aureus (1), Staphylococcus auriculares (1), Staphylococcus kloosii (1) and Staphylococcus xylosus (1). S. chromogenes corresponded to 60% of the Staphylococcus isolates (17 isolates positive on tube coagulase test). From those, 83.33% (30/36) of them were biofilm producers. This virulence factor had no association with the plasma coagulation phenotype. The identification of these microorganisms is important for the elucidation of the bovine mastitis etiology. The high percentage of S. chromogenes, biofilm producers, isolated from cows with mastitis is an important finding. This may reveal a profile change on the colonization of etiologic agents that cause this disease.
DOI: 10.6084/m9.figshare.7482515.v1
title: SWINE WASTEWATER NITROGEN REMOVAL AT DIFFERENT C/N RATIOS USING THE MODIFIED LUDZACK-ETTINGER PROCESS

description: ABSTRACT The swine industry has been presented strong growth and thus is producing large volumes of effluents that must be correctly management. Nitritation/denitritation process can be used to improve system performance, saving the energy cost with aeration and with the external source of carbon due to the lower C/N requirement. The aim of this study was to evaluate swine wastewater Nitrification/Denitrification (NDF) and Nitritation/denitritation (NDT) processes at different C/N ratios, using the Modified Ludzack-Ettinger (MLE) process, in order to obtain a system with efficient nitrogen removal and low oxygen and carbon consumption. Four phases were conducted during the experiment: Phase I) DO between 2.0-3.0 mg L−1 of O2 and C/N 1.5 (NDF); Phase II) DO between 0.6-0.7 mg L−1 of O2 and C/N 1.5 (NDT); Phase III) DO between 0.6-0.7 mg L−1 of O2, C / N ratios of1.5, 0.9, 0.75 and 0.6 (NDT); Phase IV) DO between 2.0-3.0 mg L−1 of O2, C/N ratios of 1.5, 0.9, 0.75, and 0.6 (NDF). The best operational condition was found in the C / N ratio of 0.9 (Phase III) obtaining an optimum N removal of 86.3%. In this condition, the system saved around 26.8% in total organic carbon consumption and operated at conditions of 74% lower dissolved oxygen when compared to conventional process for N-removal. This strategy can be very useful for nitrogen removal for low carbon swine wastewater as digestate from anaerobic processes.
DOI: 10.6084/m9.figshare.7482512.v1
title: Biofilme production by Staphylococcus chromogenes isolated from milk samples from bovine with mastitis

description: ABSTRACT A total of 135 crossbred cows was studied, from ten dairy herds on the state of Acre. The purpose was to identify species of Staphylococcus isolated from the mammary quarters of cows with mastitis. Additionally, the capacity of biofilm production by the species Staphylococcus chromogenes was evaluated. The sample characterization of the isolates was performed using the MALDI TOF MS (Matrix Associated Laser Desorption-Ionization - Time of Flight - Mass Spectrometry). The following species were identified by MALDI TOFF MS: S. chromogenes (36), Staphylococcus saprophyticus (5), S. chromogenes or Staphylococcus hycus (5), Staphylococcus haemolyticus (4), Staphylococcus epidermidis (3), Staphylococcus hycus (3), Staphylococcus aureus (1), Staphylococcus auriculares (1), Staphylococcus kloosii (1) and Staphylococcus xylosus (1). S. chromogenes corresponded to 60% of the Staphylococcus isolates (17 isolates positive on tube coagulase test). From those, 83.33% (30/36) of them were biofilm producers. This virulence factor had no association with the plasma coagulation phenotype. The identification of these microorganisms is important for the elucidation of the bovine mastitis etiology. The high percentage of S. chromogenes, biofilm producers, isolated from cows with mastitis is an important finding. This may reveal a profile change on the colonization of etiologic agents that cause this disease.
DOI: 10.6084/m9.figshare.7482506.v1
title: Growth Performance, Carcass Traits and Breast Meat Fatty Acids Profile of Helmeted Guinea Fowls (Numida meleagris) Fed Increasing Level of Linseed (Linum usitatissimum) and Pumpkin Seed (Cucurbita moschata) Meals

description: ABSTRACT This study was conducted to determine the effects of dietary inclusion with linseed and pumpkin seed meals on growth performance, carcass traits and breast meat fatty acids profile of helmeted guinea fowls. A total of 120 meat-type helmeted guinea fowl females of 12 weeks of age were fed with 0 (T0), 100 (T1) and 200 (T2) g/kg of linseed (LM) and pumpkin seed meals (PSM) for 56 days, in a completely randomized design with 10 replicates per treatment and four birds per replicate. The inclusion up to 200 g/kg of LM and PSM did not affect the livability, initial live weight and feed intake (p&gt;0.05). However, final live weight and feed:gain ratio improved significantly (p&lt;0.05). The T1 increased (p&lt;0.05) the carcass yield and the T2 improved the breast weight and weight and yield of leg. Also, these experimental diets did not affect the carcass weight, breast yield and sensory quality of meat (p&gt;0.05). The oleaginous seeds (LM and PSM) decreased (p&lt;0.05) the mystic, palmitic and octadecanoic acids and the w-6/w-3 ratio, as well as increased the linoleic, a-linolenic, eicosapentanoic and docosahexanoic acids (p&lt;0.05), but did not modify the concentration of monounsaturated fatty acids (p&gt;0.05) and the eicosatrienoic and arachidonic acids in breast meat (p&lt;0.05). It is recommended the inclusion of 100 g/kg of LM and PSM to improve the live weight, weight and yield of the edible portions and the essential fatty acids in breast meat of guinea fowl, without affecting the sensory quality of the meat.
DOI: 10.7284/102941
title: INS (Inertial Navigation System) data collected during research cruise MGL1115 using a Applanix POS/MV-320 V3 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7482494
title: Dietary Supplementation of Different Levels of Phytogenic Feed Additive in Broiler Diets: The Dynamics of Growth Performance, Caecal Microbiota, and Intestinal Morphometry

description: ABSTRACT The present study was conducted to investigate the influence of different levels of dietary phytogenic feed additive (PFA) on growth performance, caecal microbiota, and intestinal morphology of broilers. A total of 480 Ross-308 one-day-old male broilers chicks (body weight 43±3 g) were randomly assigned to 32 replicate pens of four experimental groups, each experimental group consisting of 8 replicates (each replicate pen consisting of 15 chicks). A basal diet was formulated based on corn and soybean meal that was fed to the control group. Other dietary treatments received a commercial PFA at 100 mg/kg (PFA100), 125 mg/kg (PFA125), and 150 mg/kg (PFA150). Body weight gain, feed intake, and feed conversion rate of broilers were recorded on 1-21, 22-42, and 1-42 days of age. One bird was slaughtered on the 21st and 42nd days and caecal contents were aseptically collected. Jejunal tissue samples were also collected on the same days. Total aerobic bacteria, coliforms, Escherichia coli, and lactobacilli were counted in the caecal contents. Villus height, villus diameter, crypt depth, muscular thickness, and goblet cell number per villus were recorded. There was no difference among the dietary treatments for growth performance and caecal microbe populations at any phase. However, the dietary PFA supplementation increased the villus height, villus width, muscularis thickness, and reduced the crypt depth and goblet cell number per villus in broilers compared to those fed control diets. In conclusion, this study suggests that dietary supplementation of a PFA consisting of blend of different spices and essential oils did not improve growth performance and caecal microbial populations despite a positive improvement in the jejunal morphometry of broilers.
DOI: 10.6084/m9.figshare.7482497
title: Intervention Technologies in juvenile delinquency in Chile: following a portfolio

description: Abstract This article deals with intervention processes in young people linked to the adolescent criminal responsibility law, promulgated in Chile in 2007. Elements of Governmentality Studies are used to propose the analysis of intervention technologies. A qualitative methodology is proposed, which incorporates elements of the Actor Network Theory. Different documents and materials are analysed with the purpose of following up on an intervention instrument designed between 2009 and 2011 for the treatment of people with problematic substance use and subsequently adapted to young law offenders. The main results describe processes of translation of interests of the government agency in charge of designing the policies for the treatment and rehabilitation of the problematic use of substances and different moments of the process of development and adaptation of the intervention instrument. This is analyzed using concepts from the Actor-Network theory. It is concluded that the instrument functions as a mediator between a logic of government and the intervention strategies aimed at young law offenders, which paradoxically makes invisible the elements that participate in its network, typical of law infringement.
DOI: 10.6084/m9.figshare.7482491
title: Quantitative sensory testing in elderly: longitudinal study

description: ABSTRACT Objective: To evaluate elderly patients in a geriatric service, along with their sensory characteristics and their association with clinical aspects. Methods: This was a descriptive longitudinal study. We enrolled 36 healthy participants of both sexes in this study. The following instruments were used and evaluations performed: clinical evaluation, Mini-Mental State Exam, and quantitative sensory testing. Results: During the follow-up, there was reduction of mean corpuscular volume at each evaluation (p &lt; 0.001) and significant increase in mean corpuscular hemoglobin concentration (p &lt; 0.001). There was an increase of the olfactory (p &lt; 0.001), salty (p = 0.024), sour (p = 0.020), bitter (p = 0.001), facial cold (p = 0.019), hand cold (p = 0.004), facial tactile (p &lt; 0.001), hand tactile (p = 0.012) and facial vibration (p = 0.018) thresholds. Previous existing morbidities were associated with sensitivity changes in the individuals in this sample. Conclusion: This longitudinal study suggests that the loss of sensitivity with aging may be associated with the presence of morbidities in elders.
DOI: 10.6084/m9.figshare.7482494.v1
title: Dietary Supplementation of Different Levels of Phytogenic Feed Additive in Broiler Diets: The Dynamics of Growth Performance, Caecal Microbiota, and Intestinal Morphometry

description: ABSTRACT The present study was conducted to investigate the influence of different levels of dietary phytogenic feed additive (PFA) on growth performance, caecal microbiota, and intestinal morphology of broilers. A total of 480 Ross-308 one-day-old male broilers chicks (body weight 43±3 g) were randomly assigned to 32 replicate pens of four experimental groups, each experimental group consisting of 8 replicates (each replicate pen consisting of 15 chicks). A basal diet was formulated based on corn and soybean meal that was fed to the control group. Other dietary treatments received a commercial PFA at 100 mg/kg (PFA100), 125 mg/kg (PFA125), and 150 mg/kg (PFA150). Body weight gain, feed intake, and feed conversion rate of broilers were recorded on 1-21, 22-42, and 1-42 days of age. One bird was slaughtered on the 21st and 42nd days and caecal contents were aseptically collected. Jejunal tissue samples were also collected on the same days. Total aerobic bacteria, coliforms, Escherichia coli, and lactobacilli were counted in the caecal contents. Villus height, villus diameter, crypt depth, muscular thickness, and goblet cell number per villus were recorded. There was no difference among the dietary treatments for growth performance and caecal microbe populations at any phase. However, the dietary PFA supplementation increased the villus height, villus width, muscularis thickness, and reduced the crypt depth and goblet cell number per villus in broilers compared to those fed control diets. In conclusion, this study suggests that dietary supplementation of a PFA consisting of blend of different spices and essential oils did not improve growth performance and caecal microbial populations despite a positive improvement in the jejunal morphometry of broilers.
DOI: 10.6084/m9.figshare.7482497.v1
title: Intervention Technologies in juvenile delinquency in Chile: following a portfolio

description: Abstract This article deals with intervention processes in young people linked to the adolescent criminal responsibility law, promulgated in Chile in 2007. Elements of Governmentality Studies are used to propose the analysis of intervention technologies. A qualitative methodology is proposed, which incorporates elements of the Actor Network Theory. Different documents and materials are analysed with the purpose of following up on an intervention instrument designed between 2009 and 2011 for the treatment of people with problematic substance use and subsequently adapted to young law offenders. The main results describe processes of translation of interests of the government agency in charge of designing the policies for the treatment and rehabilitation of the problematic use of substances and different moments of the process of development and adaptation of the intervention instrument. This is analyzed using concepts from the Actor-Network theory. It is concluded that the instrument functions as a mediator between a logic of government and the intervention strategies aimed at young law offenders, which paradoxically makes invisible the elements that participate in its network, typical of law infringement.
DOI: 10.7284/102942
title: INS (Inertial Navigation System) data collected during research cruise MGL1115 using a Kongsberg Seapath 200 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7482491.v1
title: Quantitative sensory testing in elderly: longitudinal study

description: ABSTRACT Objective: To evaluate elderly patients in a geriatric service, along with their sensory characteristics and their association with clinical aspects. Methods: This was a descriptive longitudinal study. We enrolled 36 healthy participants of both sexes in this study. The following instruments were used and evaluations performed: clinical evaluation, Mini-Mental State Exam, and quantitative sensory testing. Results: During the follow-up, there was reduction of mean corpuscular volume at each evaluation (p &lt; 0.001) and significant increase in mean corpuscular hemoglobin concentration (p &lt; 0.001). There was an increase of the olfactory (p &lt; 0.001), salty (p = 0.024), sour (p = 0.020), bitter (p = 0.001), facial cold (p = 0.019), hand cold (p = 0.004), facial tactile (p &lt; 0.001), hand tactile (p = 0.012) and facial vibration (p = 0.018) thresholds. Previous existing morbidities were associated with sensitivity changes in the individuals in this sample. Conclusion: This longitudinal study suggests that the loss of sensitivity with aging may be associated with the presence of morbidities in elders.
DOI: 10.6084/m9.figshare.7482482
title: Metabolizable Energy Levels for Meat-Type Quails at Starter Phase

description: ABSTRACT This experiment was conducted to evaluate the effects of dietary nitrogen-corrected apparent metabolizable energy (AMEn) levels on meat-type quail performance and carcass traits from 1 to 14d of age. A total of 1120 not sexed meat-type quails were randomly distributed to seven treatments, with eight replicates with 20 quails each. A basal corn and soybean meal-based diet was formulated to meet or exceed quail nutritional requirements, except for ME. Graded levels of soybean oil were added to the basal diet in replacement, to sand, to obtain dietary treatments (2,600; 2,700; 2,800; 2,900; 3,000; 3,100 and 3,200 KcalAMEn/kg diet). Data were analyzed as one-way ANOVA and optimum AMEn levels were estimated using polynomial regression model. Increasing in dietary AMEn levels elicited a linear decrease (p&lt;0.01) in feed intake and nutrient intake (AMEn, protein and lysine). Quail weight gain and final body weight exhibited a quadratic response (p&lt;0.03) to increased AMEn levels, being both optimized at 2820 KcalAMEn/kg diet. Graded AMEn levels elicited a linear increase (p&lt;0.01) in carcass dry matter and fat content, whereas moisture content was linearly decreased (p&lt;0.01). The protein content of the carcasses was not influenced (p&gt;0.05) by AMEn. Based on the results, the dietary AMEn level that warrants adequate performance and carcass traits of meat-type quails from 1 to 14d of age is 2,820 Kcal/kg diet.