cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/2qrr4-v4y48
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-100/rawdata/</p>Caulobacter112_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-100/rawdata/Caulobacter112_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter112_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-100/3dimage_116119/Caulobacter112_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter112.mrc.mdoc,  40.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-100/rawdata/Caulobacter112.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n4n1t-a7s25
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-291/rawdata/</p>Caulobacter268_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-291/rawdata/Caulobacter268_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter268_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-291/3dimage_116495/Caulobacter268_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter268.mrc.mdoc,  19.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-291/rawdata/Caulobacter268.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/52gwf-5gv77
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-117/rawdata/</p>Caulobacter129_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-117/rawdata/Caulobacter129_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter129_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-117/3dimage_116152/Caulobacter129_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter129.mrc.mdoc,  44.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-117/rawdata/Caulobacter129.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.tg2wpp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bromus ferronii",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011912-250827131500795/datasets/export for details.
DOI: 10.22002/49y5t-c3153
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-190/rawdata/</p>Caulobacter202_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-190/rawdata/Caulobacter202_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter202_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-190/3dimage_116294/Caulobacter202_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter202.mrc.mdoc,  44.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-190/rawdata/Caulobacter202.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2th5kx
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bromus ferronii",
 "Country is Czechia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011913-250827131500795/datasets/export for details.
DOI: 10.22002/knb5c-1b089
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-276/rawdata/</p>Caulobacter326_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-276/rawdata/Caulobacter326_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter326_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-276/3dimage_116458/Caulobacter326_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter326.mrc.mdoc,  42.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-276/rawdata/Caulobacter326.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1sffp-8es23
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-299/rawdata/</p>Caulobacter269_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-299/rawdata/Caulobacter269_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter269_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-299/3dimage_116511/Caulobacter269_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter269.mrc.mdoc,  42.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-299/rawdata/Caulobacter269.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/xhc-870-w38
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/645jy-g9s23
title: WT tomogram data

description: Raw data files of WT tomogram data
description: <strong>Tilt Series Date:</strong> 2020-12-28</p>
<strong>Data Taken By:</strong> Rachel Rosenzweig</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Patrick Viollier</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated a single colony from a 2% M2G agar plate in 5mL M2G at 30C, 220rpm for 24hr. Diluted each 5mL culture in 15mL M2G at 30C, 220rpm for 3hrs to OD600 0.7. Transferred to a conical centrifuge tube. Centrifuged at 6,400xg at 4C for 10min. (Media and tubes now on ice). Aspirated supernatant, resuspended with 1mL cold 1X M2. Transferred to a new 2mL microcentrifuge tube. Centrifuged at 15,000xg at 4C for 3min. Aspirated supernatant, resuspended with 900uL cold 1X M2. Added and mixed 900uL cold Percoll® [Sigma-Aldrich #P4937]. Centrifuged at 15,000xg at 4C for 20min. Aspirated top stalked cells band, collected bottom swarmer band. Transferred to a new 1.5mL microcentrifuge tube. Washed cells by adding 1mL cold 1X M2. Centrifuged at 15,000xg at 4C for 3min. (**Repeated wash 3x). Aspirated supernatant to leave 20uL cell solution resuspended.</p>
<strong>Sample Preparation:</strong> Mixed 1mL 10nm Au with 250uL 5% BSA. Vortexed. Centrifuged at 18,000xg for 30min. Aspirated supernatant to leave 20uL Au solution resuspended. Mixed 1:1 ratio of Au:Cells. Vitrobot 3uL of solution @ RT with 100% humidity. Blot force: 2. Blot time: 4s. Quantifoil R3.5/1 200 Mesh grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-62/rawdata/</p>Caulobacter68_movies.mrc,  Tilt Series (Pixel Size 0.33 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-62/rawdata/Caulobacter68_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter68_movies_full.rec,  Reconstruction (Pixel Size 1.32 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-62/3dimage_116078/Caulobacter68_movies_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Caulobacter68.mrc.mdoc,  44.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rrb2020-12-28-62/rawdata/Caulobacter68.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.w2wxk6
title: Occurrence Download

description: A dataset containing 151 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bromus ferronii",
 "Country is Denmark",
 "HasCoordinate is true"
 ]
}

 The dataset includes 151 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011914-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.