DOI: 10.5061/dryad.rfj6q578w
title: Data from: Tropical deforestation reduces plant mating quality by shifting the functional composition of pollinator communities

description: Deforestation can impact the quality of pollen received by target plants
 (i.e., delivery of incompatible pollen, self-pollen, or pollen
 from closely related individuals). Such reductions in plant mating
 quality may be direct,
 when deforestation reduces plant population size and
 the availability of pollen donors, or indirect, when
 decreased mating quality results, for
 example, from shifts in the composition of the pollinator
 community. As most flowering plants depend on animal pollinators for
 reproduction, there is a need to understand the direct and indirect links
 between deforestation, pollinator community composition,
 and plant mating quality. We quantified the direct,
 pollen-donor-mediated and indirect, pollinator-mediated effects
 of deforestation on mating
 quality in Heliconia tortuosa, a tropical herb
 pollinated by low- and high-mobility hummingbirds. We used a confirmatory
 path analysis to test the hypothesis
 that deforestation (amount of forest cover and forest
 patch size) influenced mating quality (haplotype
 diversity of pollen pools, outcrossing, and biparental inbreeding)
 directly and indirectly through functional shifts in the composition of
 pollinator communities (proportion of high-mobility hummingbirds). We
 found that deforestation triggered functional shifts in
 the composition of pollinator communities, as the proportion of
 high-mobility hummingbirds increased significantly with the amount of
 forest cover and forest patch size. The composition of the pollinator
 community affected mating quality, as the haplotype diversity of
 pollen pools increased significantly with the proportion of high-mobility
 hummingbirds, while biparental inbreeding decreased significantly.
 Although we did not detect any significant direct, pollen-donor-mediated
 effects of deforestation on mating quality,
 reductions in the amount of forest cover and forest patch size resulted in
 functional shifts that filtered out high-mobility hummingbirds from the
 pollinator community, thereby reducing mating
 quality indirectly.
 Synthesis. Deforestation primarily
 influenced plant mating quality through a cascading
 effect mediated by functional shifts in the composition of the pollinator
 community. Our results indicate that plant mating
 quality strongly depends on the composition of local pollinator
 communities. Functional shifts that filter out highly mobile and effective
 pollinators may reduce the transfer of genetically diverse pollen loads
 from unrelated plants. Such shifts may have pronounced effects on plant
 population dynamics and disrupt genetic connectivity.
description: This folder contains the raw input data and R scripts necessary
 to: (1) estimate the haplotype diversity (h) of pollen pools, outcrossing
 rates (tm), and biparental inbreeding (tm-ts); (2) estimate the proportion
 of high-mobility hummingbirds across focal forest patches; (3) run the
 confirmatory path analysis based on a piecewise SEM approach; and (4)
 recreate the main figures (graphs) of the paper. This folder contains the
 following files: <strong>1.
 Data_Moms_Locations.csv</strong> Spatial
 coordinates for all sampled maternal plants.
 <strong>2. Data_Moms.csv</strong>
 Summary data for all sampled maternal plants. For each sampled
 maternal plant, this file includes deforestation variables (amount of
 forest cover and forest patch size), data on the local pollinator
 community composition (proportion of high-mobility hummingbirds), and
 plant mating quality surrogates (haplotype diversity (h) of pollen pools,
 outcrossing rates (tm) and biparental inbreeding (tm-ts)).
 <strong>3. Data_Patches.csv</strong>
 Summary data for all sampled focal forest patches. For each focal
 forest patch, this file includes deforestation variables (amount of forest
 cover and forest patch size) and data on the local pollinator community
 composition (proportion of high-mobility hummingbirds).
 <strong>4. Genotypes.csv</strong>
 Genetic data (11 microsatellites) for all sampled maternal plants
 and selected seeds. The column “ID” is composed by the ID of each focal
 forest patch (first two numbers), followed be the ID of each maternal
 plant (letter character) and sampling year. The column “OffID” denotes
 whether a sample corresponds to a maternal plant (OffID = 0) or a seed
 (OffID ≠ 0). <strong>5.
 Graphs.Rmd</strong> R script necessary to
 recreate the main graphs (Figures 1 & 2) in the main text of the
 paper, plus Supplementary Figures 2 & 3.
 <strong>6.
 Haplotype_Diversity_Pollen_Moms.csv</strong> This
 file contains the estimated haplotype diversity (h) of pollen pools for
 each sampled maternal plant. <strong>7.
 Hummingbird_Data.csv</strong> Raw hummingbird
 capture data with mist nets. Summary data for each focal forest patch and
 number of unique hummingbird captures (identified to the species
 level). <strong>8.
 piecewiseSEM.Rmd</strong> R script necessary to
 run the confirmatory path analysis based on a piecewise SEM
 approach. <strong>9.
 Pollen_Pool.Rmd</strong> R script with function
 used to calculate the haplotype diversity (h) of pollen pools for each
 sampled maternal plant. <strong>10.
 Pollinator_Community.Rmd</strong> R script
 necessary to estimate the proportion of high-mobility hummingbirds per
 each focal forest patch (including small-sample size
 correction). <strong>11.
 Proportion_High_Mobility_Pollinators.csv</strong>
 Summary data for each focal forest patch. The total number of
 captures per species are shown for each forest patch, as well as the total
 number of high-mobility hummingbird captures and the total number of
 hummingbird captures. The proportion of high-mobility hummingbirds per
 forest patch (after small-sample size correction) is shown. Hummingbird
 species codes: CHRH (Amazilia decora), SNOB (Amazilia edward), RTAH
 (Amazilia tzacatl), VISA (Campylopterus hemileucurus), GCBR (Heliodoxa
 jacula), SBRH (Phaeochroa cuvierii), GREH (Phaethornis guy), LBIH
 (Phaethornis longirostris), and STHR (Phaethornis striigularis).