cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7743599
title: Does the nitrogen application associated with Azospirillum brasilense inoculation influence corn nutrition and yield?

description: ABSTRACT The aim of this study was to investigate the synergistic effect between inoculation with Azospirillum brasilense and nitrogen application, thus enabling a higher efficiency of nitrogen fertilization, as evaluated by nutritional value, components production, and grain yield of irrigated corn. The experiment was conducted in Selvíria, MS, Brazil, under a no-till system, on an Oxisol in the Brazilian Cerrado. The experiment was set up in a randomized block design with four replications, in a 4 × 2 factorial arrangement: four patterns of nitrogen application [application of 30 kg ha-1 of N at sowing and 150 kg ha-1 as cover (30 + 150); 30 kg ha-1 of N at sowing, split into two applications of 75 kg ha-1 as cover (30 + 75 + 75); 180 kg ha-1 of N at sowing (180); and 150 kg ha-1 of N at sowing and 30 kg ha-1 as cover (150 + 30)]; with and without inoculation of the seeds with A. brasilense. The application of 30 kg ha-1 of N at sowing and a single application of 150 kg ha-1 or two applications of 75 kg ha-1 in topdressing, inoculated with Azospirillum brasilense provided better nutrition and development, with a positive reflection on irrigated corn grain yield in the Brazilian Cerrado.
DOI: 10.6084/m9.figshare.7743602.v1
title: Production and evaluation of briquettes from urban pruning residue and sugarcane bagasse

description: ABSTRACT The search for new alternatives in order to reuse residues is one way to minimize environmental impacts by promoting sustainable practices. This study analyzed the technical feasibility of briquettes production from urban pruning residue and sugarcane bagasse, turning them into a product with higher added value besides directing their use and reducing their improper disposal. Five treatments were studied from different ratios of the two residues: T1, T2, T3, T4 and T5, respectively made of 100% of urban pruning; 50% urban pruning and 50% sugarcane bagasse; 25% urban pruning and 75% sugarcane bagasse; 10% urban pruning and 90% sugarcane bagasse; 100% sugarcane bagasse. The materials were characterized physically, chemically and energetically. Briquettes were produced and evaluated for mechanical performance, with expansion tests, mechanical resistance and friability to verify the quality of the final product. The treatments 1 and 2 showed the best results, with lower friability and average values of mechanical resistance of 167.14 and 107.56 kgf, respectively. It is concluded that both residues (sugarcane bagasse and urban pruning) had potential for briquette production, as well as the mixture between both materials.
DOI: 10.7284/127499
title: Winch data collected during research cruise AE1628 using a Markey DUSH-5 instrument system onboard the platform RV Atlantic Explorer

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743593
title: Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI

description: Abstract We assessed levels of plasma selenium (Se), selenoproteins and their change after Se supplementation in patients with mucopolysaccharidosis (MPS) types I, II and VI. This was done in a retrospective study of the medical records of 30 patients with MPS I (n=13), MPS II (n=9) and MPS VI (n=8) who were being treated with enzyme replacement therapy. As part of routine nutritional monitoring, Se levels were measured, revealing that 28 patients (93.3%) had values below the normal range. Therefore, they received supplementation for 12 months, and Se was measured after 6 and 12 months. Glutathione peroxidase (GPx) activity, total glutathione (GSHt), oxidized glutathione (GSSG) and reduced glutathione (GSH) were measured at baseline and 6 months after Se supplementation. The mean GSHt at baseline was 7.90 ± 2.36 μmol/g Hb, and after Se supplementation it was 5.76 ± 1.13 μmol/g Hb; GSH/GSSG was 2.3 ± 1.16 at baseline and 0.58 ± 0.38 after supplementation. GPx activity was 16.46 ± 3.31 U/g Hb at baseline and 4.53 ± 4.92 U/g Hb after Se supplementation. The difference was shown to be statistically significant by paired t-test. In conclusion, our study demonstrated that oxidative stress parameters were altered by Se supplementation in patients with MPS I, II and VI who were previously deficient in Se.
DOI: 10.6084/m9.figshare.7743593.v1
title: Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI

description: Abstract We assessed levels of plasma selenium (Se), selenoproteins and their change after Se supplementation in patients with mucopolysaccharidosis (MPS) types I, II and VI. This was done in a retrospective study of the medical records of 30 patients with MPS I (n=13), MPS II (n=9) and MPS VI (n=8) who were being treated with enzyme replacement therapy. As part of routine nutritional monitoring, Se levels were measured, revealing that 28 patients (93.3%) had values below the normal range. Therefore, they received supplementation for 12 months, and Se was measured after 6 and 12 months. Glutathione peroxidase (GPx) activity, total glutathione (GSHt), oxidized glutathione (GSSG) and reduced glutathione (GSH) were measured at baseline and 6 months after Se supplementation. The mean GSHt at baseline was 7.90 ± 2.36 μmol/g Hb, and after Se supplementation it was 5.76 ± 1.13 μmol/g Hb; GSH/GSSG was 2.3 ± 1.16 at baseline and 0.58 ± 0.38 after supplementation. GPx activity was 16.46 ± 3.31 U/g Hb at baseline and 4.53 ± 4.92 U/g Hb after Se supplementation. The difference was shown to be statistically significant by paired t-test. In conclusion, our study demonstrated that oxidative stress parameters were altered by Se supplementation in patients with MPS I, II and VI who were previously deficient in Se.
DOI: 10.6084/m9.figshare.7743599.v1
title: Does the nitrogen application associated with Azospirillum brasilense inoculation influence corn nutrition and yield?

description: ABSTRACT The aim of this study was to investigate the synergistic effect between inoculation with Azospirillum brasilense and nitrogen application, thus enabling a higher efficiency of nitrogen fertilization, as evaluated by nutritional value, components production, and grain yield of irrigated corn. The experiment was conducted in Selvíria, MS, Brazil, under a no-till system, on an Oxisol in the Brazilian Cerrado. The experiment was set up in a randomized block design with four replications, in a 4 × 2 factorial arrangement: four patterns of nitrogen application [application of 30 kg ha-1 of N at sowing and 150 kg ha-1 as cover (30 + 150); 30 kg ha-1 of N at sowing, split into two applications of 75 kg ha-1 as cover (30 + 75 + 75); 180 kg ha-1 of N at sowing (180); and 150 kg ha-1 of N at sowing and 30 kg ha-1 as cover (150 + 30)]; with and without inoculation of the seeds with A. brasilense. The application of 30 kg ha-1 of N at sowing and a single application of 150 kg ha-1 or two applications of 75 kg ha-1 in topdressing, inoculated with Azospirillum brasilense provided better nutrition and development, with a positive reflection on irrigated corn grain yield in the Brazilian Cerrado.
DOI: 10.6084/m9.figshare.7743587
title: Evaluation of Galectin-3 and Myocardial Fibrosis in Patients with Hypertrophic Cardiomyopathy

description: Abstract Background: Galectin-3 is the designation given to the protein that binds to ß-galactosides, expressed by activated macrophages and described as a cardiac fibrosis mediator. In hypertrophic cardiomyopathy (HCM), myocardial fibrosis is an independent predictor of adverse outcome; however, the association between Galectin-3 and myocardial fibrosis has not been studied in this cardiopathy. Objective: To evaluate the association of Galectin-3 and the presence of myocardial fibrosis in a patient with hypertrophic cardiomyopathy. Methods: Galectin-3 was measured in automated equipment using the Elisa technique in 100 participants divided into two groups: 50 patients with hypertrophic cardiomyopathy and 50 healthy control subjects. All patients with hypertrophic cardiomyopathy underwent magnetic nuclear resonance with the late enhancement technique to investigate myocardial fibrosis. For the statistical analysis, p values &lt; 0.05 were considered statistically significant. Results: Galectin-3 levels were low and did not show significant differences between patients with hypertrophic cardiomyopathy and the control group, 10.3 ± 3.1 ng/dL and 11.3 ± 2.6 ng/dL (p = 0.12) respectively. Myocardial fibrosis was a common finding and was identified in 84% (42/50) of patients with HCM, but no differences were observed between Galectin-3 levels when comparing patients with and without fibrosis, 10.3 ± 2.4 ng/dL and 10.1 ± 2.1 ng/dL (p = 0.59). Conclusion: The results did not show an association between Galectin-3 and myocardial fibrosis in patients with hypertrophic cardiomyopathy, suggesting that non-inflammatory mechanisms of myocardial fibrosis formation and cardiac remodeling are involved in this cardiopathy.
DOI: 10.6084/m9.figshare.7743590
title: Development and characterization of 20 polymorphic microsatellite markers for Epinephelus marginatus (Lowe, 1834) (Perciformes: Epinephelidae) using 454 pyrosequencing

description: Abstract The dusky grouper, Epinephelus marginatus, is a well-known and widespread marine fish assessed as endangered by the International Union for the Conservation of Nature. Analyzing the genetic diversity of this species is, therefore, of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first set of species-specific microsatellite loci for E. marginatus that can be applied when assessing both intra- and interpopulation genetic variation. Twenty microsatellite loci were isolated and characterized for the dusky grouper by genotyping 20 individuals obtained from the North Eastern Atlantic Ocean (n = 4) and from the South Western Atlantic Ocean (n = 16). The number of alleles per locus varied from 2 to 11, while the observed and expected heterozygosities ranged from 0.25 to 0.94 and 0.34 to 0.89, respectively. The polymorphic information content varied from moderately to highly informative. This suite of markers provides the first specific nuclear tools for E. marginatus and, thus, allows to assess with more specificity different populations’ structures.
DOI: 10.6084/m9.figshare.7743596.v1
title: Evaluation of Collagen Fibers, MMP2, MMP9, 8-OHdG and Apoptosis in the Aorta of Ovariectomized LDL Knockout Mice Submitted to Aerobic Exercise

description: Abstract Background: In menopause, there is greater cellular exposure to oxidative stress, related to the decreased antioxidative effects of estrogen. These metabolic changes favor the progression of cardiovascular diseases, such as atherosclerosis. Abnormal function of the aorta - the most important artery - is associated with many cardiovascular diseases. Collagen, especially types I and III, is one of the most important aortic wall components and it can be affected by many factors, including menopause. The 8-OHdG is one of the main markers of DNA oxidative damage induced by reactive oxygen species (ROS). Objective: We aimed to investigate effects of moderate aerobic training on the ascending aorta of LDL-knockout (LDL-KO) and ovariectomized female mice. Methods: A total of 15 C57BL/6 mice and 15 LDL-KO mice were divided into experimental groups. The thickness and volume density of types I and III collagen fibers were performed by morphoquantitative analysis, whereas the MMP-2 and MMP-9 and 8-OHdG were detected by immunohistochemistry and apoptosis was detected by the TUNEL assay. The significance level for all tests was p &lt; 0.05. Results: Exercise causes an increase in the thickness of the aorta in LDL-KO groups, particularly accentuated in the ovariectomized groups. The type I collagen fibers showed an increase in volume density influenced by training in both Control groups and in the LDL-KO group. Type III collagen density decreased in both groups. The MMP-2 showed moderade immunostaining in the tunica media in LDL-KO groups, which did not occur in the control groups and the MMP-9 stained irregularly in all tissues. The marker 8-OhdG was stronger in the exercise training groups. Additionally, the ovariectomy, the exercise training and the LDL-KO treatments increased apoptosis. Conclusion: These results suggest that moderate-intensity aerobic exercise in ovariectomized mice associated to an increase in LDL rate possibly increases oxidative stress and apoptosis induction.
DOI: 10.7284/109759
title: Expendable Probe data collected during research cruise AMAT01RR using a Sippican MK21 instrument system onboard the platform RV Roger Revelle

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7743581
title: Hierarchical and optimization methods for the characterization of tomato genotypes

description: ABSTRACT The correct characterization of germplasm banks is fundamental for breeders to succeed in breeding programs. Several studies have sought to obtain genotypes with resistance to pests. However, there is no consensus about which methodology is the most appropriate to characterize a germplasm bank of tomato with different levels of resistance to pests. The objective of this study was to compare methods of multivariate analysis for the evaluation of genetic diversity in tomato genotypes with different levels of resistance to pests. The experiments were conducted at the Vegetable Experimental Station of the Federal University of Uberlândia - Monte Carmelo campus (18º 42’ 43.19” South latitude and 47º 29’ 55.8” West longitude, 873 m altitude), in the period from April 2013 to November 2016. Sixteen genotypes were evaluated from the interspecific cross between LA-716 (S. pennellii) versus pre-commercial line (UFU-057) followed by backcrossing and self-fertilization, along with the pre-commercial line UFU-057 (recurrent parent) Santa Clara and the wild accession S. pennellii (donor genitor). The contents of acylsugar, foliar trichomes, South American tomato pinworm and leaf miner repellency tests were analyzed. The experimental design was the randomized block design totaling 76 plots (19 genotypes x 4 blocks). It was concluded that there was genetic variability among the evaluated genotypes. The method of graphic dispersion by principal components revealed a greater power of discrimination. Genotypes UFU-057F2RC27#4.3, UFU-057F2RC28#2.2 and UFU-057F2RC27#4.7 contain the highest levels of acylsugar, resistance to Liriomyza spp. and T. absoluta.
DOI: 10.6084/m9.figshare.7743584
title: Analysis of Iron Metabolism in Chronic Chagasic Cardiomyopathy

description: Abstract Changes in iron metabolism in heart failure (HF) have been described as an important prognostic marker. To check if the markers of iron kinetics are related to the morbidity and etiology of chagasic cardiomyopathy. Patients with Chronic Chagasic Cardiomyopathy (CCC, n = 40), with indeterminate form (IND, n = 40), besides non-chagasic cardiomyopathy (NCh, n = 40). The mean age was 50.98 ± 5.88 in CCC, 50% were male, 49.68 ± 5.28 in IND, 52.2% were male, and 49.20 ± 10.09 in NCh, 12.5% were male. Lower levels of iron (FeSe) were observed in the CCC groups (93.15 ± 36.53), when compared to IND (125.30 ± 22.79) and NCh (114.77 ± 18.90) (p = 0.0004), lower IST transferrin saturation index in CCC (29.48 ± 6.59), when compared to IND (30.95 ± 7.06) and in the NCh group (39.70 ± 7.54) p = 0.0001), total binding capacity of the lower CTLF iron in the CCC group (297.30 ± 36.46), when compared to the IND group (196.52 ± 56.95) and the NCh group (275.18 ± 33, 48) (p = 0.0001), lower ferritin in the CCC group (134.55, 1.56-42.36), when compared to the IND group (156,25, 1,72-42,20) and the NCh group (112.95, 2.88-42.66) (p = 0.0004). It was also observed that FeSe (95% CI 1.00-1.04, p = 0.0014), IST (95% CI 1.02-1.22) (p = 0.0012) and gender (95% CI 1.07-14.43 p = 0.0038) were independently associated with the degree of ventricular dysfunction in chagasic cardiomyopathy. CCC patients showed greater change in iron metabolism regarding the indeterminate form and other forms of cariomyopathies.
DOI: 10.6084/m9.figshare.7743590.v1
title: Development and characterization of 20 polymorphic microsatellite markers for Epinephelus marginatus (Lowe, 1834) (Perciformes: Epinephelidae) using 454 pyrosequencing

description: Abstract The dusky grouper, Epinephelus marginatus, is a well-known and widespread marine fish assessed as endangered by the International Union for the Conservation of Nature. Analyzing the genetic diversity of this species is, therefore, of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first set of species-specific microsatellite loci for E. marginatus that can be applied when assessing both intra- and interpopulation genetic variation. Twenty microsatellite loci were isolated and characterized for the dusky grouper by genotyping 20 individuals obtained from the North Eastern Atlantic Ocean (n = 4) and from the South Western Atlantic Ocean (n = 16). The number of alleles per locus varied from 2 to 11, while the observed and expected heterozygosities ranged from 0.25 to 0.94 and 0.34 to 0.89, respectively. The polymorphic information content varied from moderately to highly informative. This suite of markers provides the first specific nuclear tools for E. marginatus and, thus, allows to assess with more specificity different populations’ structures.
DOI: 10.6084/m9.figshare.7743578
title: Trace Element Analysis, Model-Based Clustering and Flushing to Prevent Drinking Water Contamination in Public Schools

description: Drinking water samples taken from cafeteria sinks and water fountains in each of the 76 schools in the Winston-Salem/Forsyth County Schools (WSFCS) district (North Carolina, United States) were analyzed by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) to determine As, Cd, Cr, Cu, Pb, Sb, Se and Tl. All samples from currently active schools tested below the maximum contaminant level (MCL) set for each element. Model-based clustering was employed to identify schools more prone to drinking water contamination. This multivariate approach may be used in a prevention program that can be tailored to specific school districts, with each school tested at a frequency compatible with its contamination risk level. Water flow stagnation during the summer break results in higher elemental concentrations in school drinking water, but a simple 5-60 min flushing procedure significantly reduces the contamination levels.
DOI: 10.6084/m9.figshare.7743581.v1
title: Hierarchical and optimization methods for the characterization of tomato genotypes

description: ABSTRACT The correct characterization of germplasm banks is fundamental for breeders to succeed in breeding programs. Several studies have sought to obtain genotypes with resistance to pests. However, there is no consensus about which methodology is the most appropriate to characterize a germplasm bank of tomato with different levels of resistance to pests. The objective of this study was to compare methods of multivariate analysis for the evaluation of genetic diversity in tomato genotypes with different levels of resistance to pests. The experiments were conducted at the Vegetable Experimental Station of the Federal University of Uberlândia - Monte Carmelo campus (18º 42’ 43.19” South latitude and 47º 29’ 55.8” West longitude, 873 m altitude), in the period from April 2013 to November 2016. Sixteen genotypes were evaluated from the interspecific cross between LA-716 (S. pennellii) versus pre-commercial line (UFU-057) followed by backcrossing and self-fertilization, along with the pre-commercial line UFU-057 (recurrent parent) Santa Clara and the wild accession S. pennellii (donor genitor). The contents of acylsugar, foliar trichomes, South American tomato pinworm and leaf miner repellency tests were analyzed. The experimental design was the randomized block design totaling 76 plots (19 genotypes x 4 blocks). It was concluded that there was genetic variability among the evaluated genotypes. The method of graphic dispersion by principal components revealed a greater power of discrimination. Genotypes UFU-057F2RC27#4.3, UFU-057F2RC28#2.2 and UFU-057F2RC27#4.7 contain the highest levels of acylsugar, resistance to Liriomyza spp. and T. absoluta.
DOI: 10.6084/m9.figshare.7743584.v1
title: Analysis of Iron Metabolism in Chronic Chagasic Cardiomyopathy

description: Abstract Changes in iron metabolism in heart failure (HF) have been described as an important prognostic marker. To check if the markers of iron kinetics are related to the morbidity and etiology of chagasic cardiomyopathy. Patients with Chronic Chagasic Cardiomyopathy (CCC, n = 40), with indeterminate form (IND, n = 40), besides non-chagasic cardiomyopathy (NCh, n = 40). The mean age was 50.98 ± 5.88 in CCC, 50% were male, 49.68 ± 5.28 in IND, 52.2% were male, and 49.20 ± 10.09 in NCh, 12.5% were male. Lower levels of iron (FeSe) were observed in the CCC groups (93.15 ± 36.53), when compared to IND (125.30 ± 22.79) and NCh (114.77 ± 18.90) (p = 0.0004), lower IST transferrin saturation index in CCC (29.48 ± 6.59), when compared to IND (30.95 ± 7.06) and in the NCh group (39.70 ± 7.54) p = 0.0001), total binding capacity of the lower CTLF iron in the CCC group (297.30 ± 36.46), when compared to the IND group (196.52 ± 56.95) and the NCh group (275.18 ± 33, 48) (p = 0.0001), lower ferritin in the CCC group (134.55, 1.56-42.36), when compared to the IND group (156,25, 1,72-42,20) and the NCh group (112.95, 2.88-42.66) (p = 0.0004). It was also observed that FeSe (95% CI 1.00-1.04, p = 0.0014), IST (95% CI 1.02-1.22) (p = 0.0012) and gender (95% CI 1.07-14.43 p = 0.0038) were independently associated with the degree of ventricular dysfunction in chagasic cardiomyopathy. CCC patients showed greater change in iron metabolism regarding the indeterminate form and other forms of cariomyopathies.