cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19968395.v1
title: Prepartum administration of recombinant bovine somatotropin (rBST) on adaptation to subclinical ketosis of the ewes and performance of the lambs

description: The aim of this study was to determine the effect of prepartum rbST injection on the metabolic profile of pregnant ewes induced to subclinical ketosis, as well as the metabolism until seven days of life and weight gain until seven weeks of life of the lambs. Twenty seven pregnant ewes of the pantaneiro genetic group were used, divided into two groups: rbST group (n = 14) and control group (n = 13). The rbST group received two applications of 1 mg/kg of rbST, at 97 and 111 days gestation, while the control group received placebo injections. There were significant differences between groups in levels of GGT in the ketosis post induction period and BHB concentrations in the postpartum period. Concentrations of glucose, urea, phosphorus, albumin, cholesterol, AST, NEFA and insulin were not different between dams from the two groups in different periods of the study (P&gt;0.05). There was an effect of rbST on body weight observed already at fourteen days of life (P&lt;0.0001), there was an increase in serum phosphorus levels at birth of lambs (P=0.0014), and albumin at seven days of life (P = 0.0014) of the lambs, with no difference between groups for the other metabolites. Therefore, the use of rbST was effective in increasing the weight of the lambs until the seventh week of life. In addition, rbST treatment had positive effects on the dam metabolism with reduction of liver overload, as indicated by decreased GGT after ketosis induction and decreased BHB at the postpartum period.
DOI: 10.6084/m9.figshare.19968398
title: Evaluation of diet formulated with cassava by-products in the rabbit semen production

description: The quantitative and qualitative rabbit semen characteristics fed with a reference diet and a diet formulated with 79.83% of cassava by-products were evaluated. 20 New Zealand White rabbit bucks, with initial average age of seven months were used, individually allocated and distributed in a completely ramdomized design, with two diets (treatments) and ten replications. The ten semen collections per animal were taken during six months. The evaluated parameters were: semen volume without gel and gel volume, color of semen, pH, spermatic progressive motility, spermatic vigor, spermatic concentration, spermatozoa number in the ejaculation, normal spermatozoa, abnormal and primary and secondary abnormalities spermatozoa. The semen volume without gel, the normal spermatozoa, primary and secondary abnormalities spermatozoa were similar (P&gt;0.05) to the animals fed with cassava by-products diet and reference diet. Other semen parameters were better (P&lt;0.05) in the animals fed with the cassava by-products diet. The results of the quali-quantitative characteristics of the semen from New Zealand White rabbits showed that it is possible to use diets containing 79.83% of inclusion of cassava by-products in the diet of rabbit bucks, without affecting semen characteristics, since we observed the reduced level of tannins and hydrocyanic acid.
DOI: 10.6084/m9.figshare.19968398.v1
title: Evaluation of diet formulated with cassava by-products in the rabbit semen production

description: The quantitative and qualitative rabbit semen characteristics fed with a reference diet and a diet formulated with 79.83% of cassava by-products were evaluated. 20 New Zealand White rabbit bucks, with initial average age of seven months were used, individually allocated and distributed in a completely ramdomized design, with two diets (treatments) and ten replications. The ten semen collections per animal were taken during six months. The evaluated parameters were: semen volume without gel and gel volume, color of semen, pH, spermatic progressive motility, spermatic vigor, spermatic concentration, spermatozoa number in the ejaculation, normal spermatozoa, abnormal and primary and secondary abnormalities spermatozoa. The semen volume without gel, the normal spermatozoa, primary and secondary abnormalities spermatozoa were similar (P&gt;0.05) to the animals fed with cassava by-products diet and reference diet. Other semen parameters were better (P&lt;0.05) in the animals fed with the cassava by-products diet. The results of the quali-quantitative characteristics of the semen from New Zealand White rabbits showed that it is possible to use diets containing 79.83% of inclusion of cassava by-products in the diet of rabbit bucks, without affecting semen characteristics, since we observed the reduced level of tannins and hydrocyanic acid.
DOI: 10.6084/m9.figshare.19968401
title: Quantification of ruminal microbiota and production of methane and carbonic dioxide from diets with inclusion of glycerin

description: The aim of this study was to quantify the liquid-associated bacteria and protozoa of the rumen and to evaluate the potential of in vitroproduction of gases CH4 and CO2 of diets with inclusion of glycerin for sheep. Two diets were formulated with a roughage:concentrate ratio of 20:80, according to the presence or absence of glycerin: G0 - control, without glycerin; and G10 - 10% glycerin as part of the concentrate. To quantify the liquid-associated bacteria and protozoa from the rumen and production of CH4 and CO2 gases, a completely randomized design was used. The diets had no effect (P&gt;0.05) on production and composition of liquid-associated protozoa and bacteria from the rumen of lambs. Likewise, a (P&gt;0.05) diet effect was not observed on production of CH4 and CO2in vitro, which presented average concentrations of 24.55mL/g MS and 51.52mL/g MS, respectively. The use of 10% glycerin did not alter production or composition of ruminal microflora, and moreover, did not negatively impact the production of CH4 and CO2 gases in vitro.
DOI: 10.6084/m9.figshare.19968401.v1
title: Quantification of ruminal microbiota and production of methane and carbonic dioxide from diets with inclusion of glycerin

description: The aim of this study was to quantify the liquid-associated bacteria and protozoa of the rumen and to evaluate the potential of in vitroproduction of gases CH4 and CO2 of diets with inclusion of glycerin for sheep. Two diets were formulated with a roughage:concentrate ratio of 20:80, according to the presence or absence of glycerin: G0 - control, without glycerin; and G10 - 10% glycerin as part of the concentrate. To quantify the liquid-associated bacteria and protozoa from the rumen and production of CH4 and CO2 gases, a completely randomized design was used. The diets had no effect (P&gt;0.05) on production and composition of liquid-associated protozoa and bacteria from the rumen of lambs. Likewise, a (P&gt;0.05) diet effect was not observed on production of CH4 and CO2in vitro, which presented average concentrations of 24.55mL/g MS and 51.52mL/g MS, respectively. The use of 10% glycerin did not alter production or composition of ruminal microflora, and moreover, did not negatively impact the production of CH4 and CO2 gases in vitro.
DOI: 10.6084/m9.figshare.19968402
title: Double-setting alpha-tricalcium phosphate cement provided with interconection channels in rabbits after enucleation: A potential implant for the anophthalmic socket

description: The purpose of this study was to evaluate the macroscopy and microstructure of a double setting alpha-tricalcium phosphate bone cement sphere provided with interconnection channels (alpha-TCP-i), as well as the integration of the implant with the rabbits' orbital tissue, through macroscopic analysis and histopathology. The external and internal surfaces of the alpha-TCP-i were evaluated macroscopically and by electron microscopy. Twelve New Zealand rabbits received 12mm implants of alpha-TCP-i following enucleation of the left eye. The clinical assessment was undertaken daily during the first 15 days, followed by fortnightly assessment until the end of the study period. For the morphological analysis, exenteration was performed in 3 animals per experimental period (15, 45, 90 and 180 days). The external and internal surfaces of the implant appeared solid, smooth and compact, with six channels which interconnected centrally. The micro-architecture was characterized by the formation of columns of hexagonal crystals. No signs of infection, exposure, dehiscence of sutures or extrusion of the implant were noted in any of the animals during the entire period of the study. The morphological evaluation demonstrated the presence of a thin capsule around the implant, from whence appeared fibro-vascular projections, which penetrated it through the interconnecting channels. In the first days after the insertion of the implant, an intense inflammatory reaction was noted. At 180 days, however, there were no signs of inflammation. The alpha-tricalcium phosphate cement implant was well tolerated in this rabbit model and appeared to be relatively inert with some fibrovascular ingrowth through the large channels.
DOI: 10.6084/m9.figshare.19968402.v1
title: Double-setting alpha-tricalcium phosphate cement provided with interconection channels in rabbits after enucleation: A potential implant for the anophthalmic socket

description: The purpose of this study was to evaluate the macroscopy and microstructure of a double setting alpha-tricalcium phosphate bone cement sphere provided with interconnection channels (alpha-TCP-i), as well as the integration of the implant with the rabbits' orbital tissue, through macroscopic analysis and histopathology. The external and internal surfaces of the alpha-TCP-i were evaluated macroscopically and by electron microscopy. Twelve New Zealand rabbits received 12mm implants of alpha-TCP-i following enucleation of the left eye. The clinical assessment was undertaken daily during the first 15 days, followed by fortnightly assessment until the end of the study period. For the morphological analysis, exenteration was performed in 3 animals per experimental period (15, 45, 90 and 180 days). The external and internal surfaces of the implant appeared solid, smooth and compact, with six channels which interconnected centrally. The micro-architecture was characterized by the formation of columns of hexagonal crystals. No signs of infection, exposure, dehiscence of sutures or extrusion of the implant were noted in any of the animals during the entire period of the study. The morphological evaluation demonstrated the presence of a thin capsule around the implant, from whence appeared fibro-vascular projections, which penetrated it through the interconnecting channels. In the first days after the insertion of the implant, an intense inflammatory reaction was noted. At 180 days, however, there were no signs of inflammation. The alpha-tricalcium phosphate cement implant was well tolerated in this rabbit model and appeared to be relatively inert with some fibrovascular ingrowth through the large channels.
DOI: 10.6084/m9.figshare.19968404.v1
title: Performance of meat type quails in diallel cross

description: The aim of this study was to evaluate the performance of four strains of meat type quails, designated L1, L2, L3 and L4 in diallel crosses. This study was carried out at the premises of the Genetic Improvement Program for Quails of UFVJM, from December 2011 to May 2012. The average body weight at hatch, at 35 and at 42 days old, the average daily feed intake at 35 and at 42 days old, and feed conversion at 35 and 42 days old were evaluated. Diallel analyzes were developed using univariate analyses, considering the methodology of complete diallel, from the average of the genotype combinations resulting in a complete randomized design with three replications. The combining ability analysis revealed significance for general combining ability for all traits, except for average feed intake and feed conversion at 35 days old. There was significant effect of specific combining ability for hatch weight (L2xL3; L2xL4; L1xL4); feed conversion from hatch to 35 days of age (L1xL3; L2xL4), and from hatch to 42 days (L1xL3; L2xL3; L2xL4); and average feed intake from hatch to 35 days of age (L1xL3; L2xL3), thus allowing the identification of more favorable hybrid combinations. The reciprocal effect showed significance only for hatch weight. Based on general combining ability, the strains which are more favorable for increased body weight were L1 and L2. For feed conversion, for the specific combining ability, the crosses L1xL3 and L2xL4 showed the best results, while for feed intake from hatch to 35 days old, the crosses L1xL3 and L2xL3 would be the most suitable.
DOI: 10.6084/m9.figshare.19968404
title: Performance of meat type quails in diallel cross

description: The aim of this study was to evaluate the performance of four strains of meat type quails, designated L1, L2, L3 and L4 in diallel crosses. This study was carried out at the premises of the Genetic Improvement Program for Quails of UFVJM, from December 2011 to May 2012. The average body weight at hatch, at 35 and at 42 days old, the average daily feed intake at 35 and at 42 days old, and feed conversion at 35 and 42 days old were evaluated. Diallel analyzes were developed using univariate analyses, considering the methodology of complete diallel, from the average of the genotype combinations resulting in a complete randomized design with three replications. The combining ability analysis revealed significance for general combining ability for all traits, except for average feed intake and feed conversion at 35 days old. There was significant effect of specific combining ability for hatch weight (L2xL3; L2xL4; L1xL4); feed conversion from hatch to 35 days of age (L1xL3; L2xL4), and from hatch to 42 days (L1xL3; L2xL3; L2xL4); and average feed intake from hatch to 35 days of age (L1xL3; L2xL3), thus allowing the identification of more favorable hybrid combinations. The reciprocal effect showed significance only for hatch weight. Based on general combining ability, the strains which are more favorable for increased body weight were L1 and L2. For feed conversion, for the specific combining ability, the crosses L1xL3 and L2xL4 showed the best results, while for feed intake from hatch to 35 days old, the crosses L1xL3 and L2xL3 would be the most suitable.
DOI: 10.6084/m9.figshare.19968405
title: Species-specific identification of red-meat and meat derivative products with the PCR-RFLP technique

description: The present study aimed at evaluate the viability of PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) for identification of fraud and/or accidental contamination in buffalo meat - fresh and processed. Pure, autoclaved and controlled fraud samples, produced in the laboratory with the addition of 1, 5, 10 and 50% of beef in raw homogenized buffalo meat samples, were tested. Furthermore, different extraction methods, using a commercial kit and classical technique using phenol-chloroform, were compared. The statistical result was obtained by contingency table analyzed by chi-square and the Fisher exact test. The specificity was highly significant (p &lt;0.0001), and the sensitivity was highly significant in dilutions from 10% (p &lt;0.0001). Despite its accuracy and precision, capable to detect a contamination level of 1%, PCR-RFLP technique is not recommended for inspection in cases of accidental contamination. This is due to the need of test repetition in levels of contamination lower than 10%. The efficiency of this test is lower to autoclaved meat. The PCR-RFPL technique can be used for certification of food made with specific species (species identification certification stamp).
DOI: 10.6084/m9.figshare.19968407.v1
title: Etiologic diagnosis of diseases of the central nervous system of horses in Minas Gerais State, Brazil

description: Brazil has the fourth largest equine herd in the world and the State of Minas Gerais has the largest equine population in the country. Therefore, an accurate diagnosis of cases of neurologic diseases is a priority in Minas Gerais. The aim of this study was to identify by Polymerase Chain Reaction (PCR) infectious agents associated with neurological disease in the central nervous system (CNS) of horses. A survey of encephalitis and encephalomyelitis in horses in Minas Gerais State was performed on samples of CNS from horses that died with neurological signs from January 2009 to January 2011. Forty seven CNS samples from 217 (21.7%) horses were positive for rabies virus by the indirect immunofluorescence assay and mouse inoculation. Among the 170 samples that were negative for rabies, EHV-1 was detected in 20 (11.8%) and the swine herpesvirus-1 (SHV-1) was detected in one sample by PCR, and the Saint Louis encephalitis virus (SLEV) was identified in another sample by reverse transcription (RT) and PCR (RT-PCR). Rabies virus is the most common causative agent of encephalitis in horses, despite the increasing number of cases of encephalitis associated with EHV-1 in the State of Minas Gerais.
DOI: 10.6084/m9.figshare.19968405.v1
title: Species-specific identification of red-meat and meat derivative products with the PCR-RFLP technique

description: The present study aimed at evaluate the viability of PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) for identification of fraud and/or accidental contamination in buffalo meat - fresh and processed. Pure, autoclaved and controlled fraud samples, produced in the laboratory with the addition of 1, 5, 10 and 50% of beef in raw homogenized buffalo meat samples, were tested. Furthermore, different extraction methods, using a commercial kit and classical technique using phenol-chloroform, were compared. The statistical result was obtained by contingency table analyzed by chi-square and the Fisher exact test. The specificity was highly significant (p &lt;0.0001), and the sensitivity was highly significant in dilutions from 10% (p &lt;0.0001). Despite its accuracy and precision, capable to detect a contamination level of 1%, PCR-RFLP technique is not recommended for inspection in cases of accidental contamination. This is due to the need of test repetition in levels of contamination lower than 10%. The efficiency of this test is lower to autoclaved meat. The PCR-RFPL technique can be used for certification of food made with specific species (species identification certification stamp).
DOI: 10.6084/m9.figshare.19968407
title: Etiologic diagnosis of diseases of the central nervous system of horses in Minas Gerais State, Brazil

description: Brazil has the fourth largest equine herd in the world and the State of Minas Gerais has the largest equine population in the country. Therefore, an accurate diagnosis of cases of neurologic diseases is a priority in Minas Gerais. The aim of this study was to identify by Polymerase Chain Reaction (PCR) infectious agents associated with neurological disease in the central nervous system (CNS) of horses. A survey of encephalitis and encephalomyelitis in horses in Minas Gerais State was performed on samples of CNS from horses that died with neurological signs from January 2009 to January 2011. Forty seven CNS samples from 217 (21.7%) horses were positive for rabies virus by the indirect immunofluorescence assay and mouse inoculation. Among the 170 samples that were negative for rabies, EHV-1 was detected in 20 (11.8%) and the swine herpesvirus-1 (SHV-1) was detected in one sample by PCR, and the Saint Louis encephalitis virus (SLEV) was identified in another sample by reverse transcription (RT) and PCR (RT-PCR). Rabies virus is the most common causative agent of encephalitis in horses, despite the increasing number of cases of encephalitis associated with EHV-1 in the State of Minas Gerais.
DOI: 10.6084/m9.figshare.19968410
title: Crude gliceryn associated with sorghum silagein diets for lambs

description: This study aimed to assess the levels of crude glycerin (CG) 0, 20, 40, 60 and 80g/kg of dry matter (DM) of sorghum silage on fermentation kinetics, 0, 30, 60 and 90g/kg DM silage on the kinetics of degradation of neutral detergent fiber (NDF), and levels 0, 17, 33, 55 and 70g/kg DM silage on intake and digestibility of DM and nutritional compounds and nitrogen balance in lambs. The volume and rate of digestion of non-fiber carbohydrates (Vcnf) and (Kdcnf), respectively, showed a quadratic Vcnf being estimated maximum (126.62mL/g DM) and Kdcnf minimum (0.0782/h) for inclusion of 54.85 CG 58.75g/kg DM, respectively. Since the time of colonization (L), they presented a linear decrease of 0.013h for each 10g CG/kg. DM linearly decreased from 0.996% degradation for fraction B. There was no significant effect of the inclusion of CG on the intake and dry matter digestibility and nutritional compounds and nitrogen balance. The use of 50CG to 70g/kg DM in diets based on sorghum silage can be an alternative energy source for sheep.
DOI: 10.6084/m9.figshare.19968410.v1
title: Crude gliceryn associated with sorghum silagein diets for lambs

description: This study aimed to assess the levels of crude glycerin (CG) 0, 20, 40, 60 and 80g/kg of dry matter (DM) of sorghum silage on fermentation kinetics, 0, 30, 60 and 90g/kg DM silage on the kinetics of degradation of neutral detergent fiber (NDF), and levels 0, 17, 33, 55 and 70g/kg DM silage on intake and digestibility of DM and nutritional compounds and nitrogen balance in lambs. The volume and rate of digestion of non-fiber carbohydrates (Vcnf) and (Kdcnf), respectively, showed a quadratic Vcnf being estimated maximum (126.62mL/g DM) and Kdcnf minimum (0.0782/h) for inclusion of 54.85 CG 58.75g/kg DM, respectively. Since the time of colonization (L), they presented a linear decrease of 0.013h for each 10g CG/kg. DM linearly decreased from 0.996% degradation for fraction B. There was no significant effect of the inclusion of CG on the intake and dry matter digestibility and nutritional compounds and nitrogen balance. The use of 50CG to 70g/kg DM in diets based on sorghum silage can be an alternative energy source for sheep.