DOI: 10.5061/dryad.vq83bk454
title: Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 2

description: This dataset contains raw whole-genome sequencing data (FASTQ files) for
 wild-type and riboflavin biosynthetic gene deletion mutants
 of Mycobacterium
 tuberculosis and Mycolicibacterium smegmatis. These
 strains were generated to investigate the source of metabolic antigens
 recognized by mucosal-associated invariant T (MAIT) cells, which detect
 intermediates from the riboflavin biosynthetic pathway. To confirm
 successful gene deletions and rule out off-target effects, whole-genome
 sequencing was performed for each mutant and its corresponding wild-type
 strain. This dataset supports the verification of targeted genetic
 modifications and enables comparative genomic analyses related to MR1
 antigen production in mycobacteria.
description: # Whole genome sequence data of Mycobacterium tuberculosis and
 Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic
 pathway- Part 2 Dataset DOI:
 [10.5061/dryad.vq83bk454](10.5061/dryad.vq83bk454) ## Description of the
 data and file structure Whole Genome Sequence of wild type strain and
 riboflavin biosynthetic pathway mutants of *Mycobacterium tuberculosis*
 (Mtb) and *Mycolicibacterium smegmatis* (Msm). Also, this dataset has part
 1 which is available at https://doi.org/10.5061/dryad.4tmpg4fnv Naming
 convention for files:  For file names starting in digits 1. Strain number
 (1: Wild Type, 2: *ΔfbiC, *3: ΔribH2*, 4: *ΔribH2 ΔribH1,* 5:
 ΔribH2::ribH2, *6: *ΔfbiC::fbiC, 7: ΔribH2 ΔribH:: ribH2 ribH1*) 2. Sample
 Number 3. Read (Forward/Reverse) 6. Replicate e.g. For file name
 "7_S23_L004_R2_001"Strain number: 7 (*ΔribH2 ΔribH:: ribH2
 ribH1*); Sample Number: S23; Read: R2 (Reverse Read); Replicate: 001 OR
 For file names starting in "Dobos" 1. Lab name 2. Strain Name 3.
 Sample Number 4. Batch Number 5. Read (Forward/Reverse) 6. Replicate e.g.
 For file name "Dobos_msmegriba2comp_7_S25_L002_R2_001": Lab
 name: Dobos; Strain Name: msmegriba2comp_7; Sample Number: S25; Batch
 Number: L002; Read: R2 (Reverse Read); Replicate: 001 ### Files and
 variables #### File: 7\_S23\_L004\_R2\_001.fastq.gz
 **Description:** Reverse Read for Msm *ΔribH2 ΔribH1::ribH2 ribH1* ####
 File: 7\_S23\_L004\_R1\_001.fastq.gz **Description:** Forward Read for
 Msm *ΔribH2 ΔribH1::ribH2 ribH1* #### File: 6\_S22\_L004\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔfbiC::fbiC* #### File:
 6\_S22\_L004\_R2\_001.fastq.gz **Description:** Reverse Read for
 Msm *ΔfbiC::fbiC* #### File: 4\_S20\_L004\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribH2 ΔribH1* #### File:
 4\_S20\_L004\_R2\_001.fastq.gz **Description:** Reverse Read for
 Msm *ΔribH2 ΔribH1* #### File: 5\_S21\_L004\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribH2::ribH2* #### File:
 3\_S19\_L004\_R2\_001.fastq.gz **Description:** Forward Read for
 Msm *ΔribH2* #### File: 3\_S19\_L004\_R1\_001.fastq.gz
 **Description:**  Reverse Read for Msm *ΔribH2* #### File:
 5\_S21\_L004\_R2\_001.fastq.gz **Description:** Reverse Read for
 Msm *ΔribH2::ribH2* #### File: 1\_S17\_L004\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm Wild Type #### File:
 1\_S17\_L004\_R2\_001.fastq.gz **Description:** Reverse Read for Msm Wild
 Type #### File: 2\_S18\_L004\_R1\_001.fastq.gz **Description:** Forward
 Read for Msm *ΔfbiC* #### File: 2\_S18\_L004\_R2\_001.fastq.gz
 **Description:** Reverse Read for Msm *ΔfbiC* #### File:
 Dobos\_mtbwt\_18\_S36\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Mtb Wild Type H37Rv #### File:
 Dobos\_mtbwt\_18\_S36\_L002\_R1\_001.fastq.gz **Description:** Forward
 Read for Mtb Wild Type H37Rv #### File:
 Dobos\_mtbrv3032\_19\_S37\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Mtb Wild Type Rv3032 #### File:
 Dobos\_mtbrv3032\_19\_S37\_L002\_R1\_001.fastq.gz **Description:** Forward
 Read for Mtb Wild Type Rv3032 #### File:
 Dobos\_mtbribhko\_13\_S31\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Mtb *ΔribH* #### File:
 Dobos\_mtbribhko\_13\_S31\_L002\_R1\_001.fastq.gz **Description:** Forward
 Read for Mtb *ΔribH* #### File:
 Dobos\_mtbribhcomp\_12\_S30\_L002\_R2\_001.fastq.gz
 **Description:** Reverse Read for Mtb *ΔribH::ribH* #### File:
 Dobos\_mtbribhcomp\_12\_S30\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Mtb *ΔribH::ribH* #### File:
 Dobos\_mtbribgko\_15\_S33\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Mtb *ΔribG* #### File:
 Dobos\_mtbribgko\_15\_S33\_L002\_R1\_001.fastq.gz **Description:** Forward
 Read for Mtb *ΔribG* #### File:
 Dobos\_mtbribgcomp\_14\_S32\_L002\_R2\_001.fastq.gz
 **Description:** Reverse Read for Mtb *ΔribG::ribG* #### File:
 Dobos\_mtbribgcomp\_14\_S32\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Mtb *ΔribG::ribG* #### File:
 Dobos\_mtbribcko\_11\_S29\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Mtb *ΔribC* #### File:
 Dobos\_mtbribcko\_11\_S29\_L002\_R1\_001.fastq.gz **Description:** Forward
 Read for Mtb *ΔribC* #### File:
 Dobos\_mtbribccomp10\_S28\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Mtb *ΔribC::ribC* #### File:
 Dobos\_mtbribccomp10\_S28\_L002\_R1\_001.fastq.gz **Description:** Forward
 Read for Mtb *ΔribC::ribC*