cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/3t0f5-gm095
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-12-22</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-5/3dimage_68109/</p>11dec22_C104_1_AT3BT1_0003_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 421.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-5/3dimage_68109/11dec22_C104_1_AT3BT1_0003_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT3BT1_0003_ns.st,  Tilt Series (Pixel Size 0.5 nm), 867.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-5/rawdata/11dec22_C104_1_AT3BT1_0003_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec22_C104_1_AT3BT1_0003_ns_full.tiff,  file associated to 3D Image #68109, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-22-5/file_15841/11dec22_C104_1_AT3BT1_0003_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2bffu3
title: Occurrence Download

description: A dataset containing 88 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "OccurrenceStatus is Present",
 "TaxonKey is Aquila heliaca",
 "Year 2020-2025"
 ]
}

 The dataset includes 88 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046647-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/cfw42-94s12
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-21</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-9/rawdata/</p>tomography3.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-9/rawdata/tomography3.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography3.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-9/3dimage_47333/tomography3.rec"
        > <i class="download icon"></i> Download </a></p>

        f3.rec,  Reconstruction (Pixel Size 0.0 nm), 426.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-9/3dimage_47852/f3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4xhry-tf757
title: Rhodopseudomonas palustris TIE-1

description: Raw data files of Rhodopseudomonas palustris TIE-1
description: <strong>Tilt Series Date:</strong> 2012-05-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Rhodopseudomonas palustris</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Nicholas ShikumaDianne Newman</p>
<strong>Purification / Growth Conditions / Treatment:</strong> wild type and shc mutantgrown by photoheterotrophic anaerobic exponential and stationary</p>
<strong>Sample Preparation:</strong> vitrobot</p>
  amw2012-05-09-12_slicer4846.jpg: nested vesicle amw2012-05-09-12_slicer4848.jpg: REZ .  Granules amw2012-05-09-12_slicer4852.jpg: Unknown amw2012-05-09-12_slicer4855.jpg: Double membrane vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-12/rawdata/</p>WT_expo0009.mrc,  Tilt Series (Pixel Size 0.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-12/rawdata/WT_expo0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        WT_expo0009_part122_7.rec,  Reconstruction (Pixel Size 1.8 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-12/3dimage_18464/WT_expo0009_part122_7.rec"
        > <i class="download icon"></i> Download </a></p>

        WT_expo0009_part122_7.rec,  Reconstruction (Pixel Size 1.8 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2012-05-09-12/3dimage_18471/WT_expo0009_part122_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x0n7q-9g273
title: Myxococcus xanthus LL061

description: mCherry-bacP.First loaded in Titan cartridge and imaged in cryoLM.Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 29100x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-18/rawdata/</p>LL061_UCLA_correlated_20130424_1.mrc,  Tilt Series (Pixel Size 1.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-18/rawdata/LL061_UCLA_correlated_20130424_1.mrc"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_1_full.rec,  Reconstruction (Pixel Size 2.0 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-18/3dimage_26289/LL061_UCLA_correlated_20130424_1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        LL061_UCLA_correlated_20130424_1_bin2_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 314.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-04-24-18/3dimage_26779/LL061_UCLA_correlated_20130424_1_bin2_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j7vk7-kgp76
title: COS7

description: Cos7 - not treatment, no transfection
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cells frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and plunge frozen</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-12/3dimage_69021/</p>12jan20_C106_2_COS7neg_AT1BT1_0019_part122_4.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-12/3dimage_69021/12jan20_C106_2_COS7neg_AT1BT1_0019_part122_4.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0019.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-12/rawdata/12jan20_C106_2_COS7neg_AT1BT1_0019.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C106_2_COS7neg_AT1BT1_0019_part122_4.tiff,  file associated to 3D Image #69021, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-12/file_16094/12jan20_C106_2_COS7neg_AT1BT1_0019_part122_4.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hycm6-byc11
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns_BSKET.jpg: file associated to 3D Image #54383
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-17/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns.st,  Tilt Series (Pixel Size 0.5 nm), 891.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-17/rawdata/11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 501.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-17/3dimage_54383/11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns_BSKET.tiff,  file associated to 3D Image #54383, 698.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-17/file_13115/11dec12_cryosec_pnlegfp_grid3_Batch4_0033_ns_BSKET.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cvw3k-gw762
title: HUVECs infected w/ pNLGFP

description: HUVECs cells infected with pNLGFP and cultured additional 24 h in presence of protease inhibitor. Sample# C71
description: <strong>Tilt Series Date:</strong> 2011-06-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct from Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward. Cells infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HUVECs grown on grids and infected pNLGFP-VSVG and then cultures additional 24 h allowed virus expression in presence of protease inhibitor </p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-5/rawdata/</p>11jun04_PI_5.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-5/rawdata/11jun04_PI_5.st"
        > <i class="download icon"></i> Download </a></p>

        11jun04_PI_5_full.rec,  Reconstruction (Pixel Size 2.0 nm), 644.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-5/3dimage_68370/11jun04_PI_5_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11jun04_PI_5_fid.tiff,  file associated to 3D Image #68370, 825.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-06-04-5/file_15887/11jun04_PI_5_fid.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z2gwm-zey86
title: HeLa siRNA tfx CHMP2A/B infx pNLGFP

description: HeLa cells infected with pNLGFP and 12h post infection cells were transfected with siRNA targeting CHMP2A and CHMP2B. 18h after siRNA tfx, cells were frozen Sample# C84-2/3
description: <strong>Tilt Series Date:</strong> 2011-05-31</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP2A and 2B siRNAs provided by Wes Sundquist. pNLGFP proviral construct provided by Irvin Chen UCLA.  Virus prepared at Caltech by Cora Woodward.  Cells transfected with siRNA and then infected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and infected and siRNA transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-2/3dimage_68489/</p>11may31_4_orig_2_full.rec,  Reconstruction (Pixel Size 2.0 nm), 681.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-2/3dimage_68489/11may31_4_orig_2_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11may31_4_orig_2.st,  Tilt Series (Pixel Size 0.5 nm), 924.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-2/rawdata/11may31_4_orig_2.st"
        > <i class="download icon"></i> Download </a></p>

        11may31_4_orig_2.tiff,  file associated to 3D Image #68489, 452.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-05-31-2/file_15916/11may31_4_orig_2.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bhmtj-v0p06
title: Cryo-sectioned Jurkat VSVG-pNL4GFP

description: Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky
description: <strong>Tilt Series Date:</strong> 2011-12-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
  11dec12_cryosec_pnlegfp_grid3_Batch2_0001_full.jpg: file associated to 3D Image #54353
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-2/rawdata/</p>11dec12_cryosec_pnlegfp_grid3_Batch2_0001.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-2/rawdata/11dec12_cryosec_pnlegfp_grid3_Batch2_0001.st"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch2_0001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 404.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-2/3dimage_54353/11dec12_cryosec_pnlegfp_grid3_Batch2_0001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11dec12_cryosec_pnlegfp_grid3_Batch2_0001_full.tiff,  file associated to 3D Image #54353, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-12-12-2/file_13087/11dec12_cryosec_pnlegfp_grid3_Batch2_0001_full.tiff"
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DOI: 10.22002/tqz2b-8kq17
title: Chromogranin A-GFP

description: CLEM of chromogranin A-GFP expressing INS-1E cells
description: <strong>Tilt Series Date:</strong> 2014-08-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Chromogranin A GFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Friedberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both side pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads added to grids before freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-18/rawdata/</p>tomogram2.mrc,  Tilt Series (Pixel Size 0.96 nm), 861.6 MB  
        
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        tomogram2_part121_2.rec,  Reconstruction (Pixel Size 3.84 nm), 223.7 MB  
        
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        tomogram2.rec,  Reconstruction (Pixel Size 0.0 nm), 133.1 MB  
        
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