cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.18238806
title: LEYKOTSIT ADGEZIYASI VA DIAPEDEZI BUZILISHLARI KAPILLYAR-PERIVASKULYAR TUZILMALAR FUNKSIYASIGA TA'SIRI VA KLINIK AHAMIYATI

description: Leykotsit adgeziya va diapedez (LAD) sindromlari va funksional buzilishlar kam uchraydigan immun yetishmovchiligi holatlari bo'lib, ular leykotsitlarning endotelga yopishishi va qon tomirlaridan chiqish jarayonlarini tartibga soluvchi molekulyar mexanizmlarning buzilishi natijasida kelib chiqadi. Bu buzilishlar asosan integrinlar va selektinlar oilasiga mansub adgeziya molekulalarining yoki ularning ligandlarining defektlari bilan bog'liq. Ushbu sharhli maqola LAD va unga o'xshash holatlarning patofiziologik mexanizmlarini, xususan kapillyar-perivaskulyar birlikning normal funksiyasiga ta'sirini chuqur o'rganishga qaratilgan. So'nggi o'n yillikda (2015-2025) olib borilgan ilmiy tadqiqotlar shuni ko'rsatdiki, leykotsit endotelial o'zaro ta'sirining buzilishi nafaqat o'tkir va surunkali yallig'lanish jarayonlarini, balki to'qimalarning qayta tiklanishi, angiogenez, immun monitoring va yaralarning bitishini ham jiddiy ta'sir qiladi. LAD bilan og'rigan bemorlarda takrorlanuvchi bakteriyal infektsiyalar, periodontal kasalliklar va gipopiyon bilan kechadigan yara bitishining kechikishi kabi og'ir klinik ko'rinishlar kuzatiladi. Maqolada adgeziya molekulalarining funktsiyalari va ularning buzilishining molekulyar asoslari, hayvon modellarida olib borilgan eksperimental tadqiqotlar, yutuqlar va klinik amaliyotga oid ko'rib chiqilgan holatlar tahlil qilinadi. Bundan tashqari, ushbu sohadagi zamonaviy diagnostika usullari (oq qon hujayralarining adgeziya qobiliyatini tekshirish, oqsil ekspressiyasining o'lchovi, genetik tahlillar) va potentsial terapevtik yondashuvalar (gen terapiyasi, ferment almashtirish terapiyasi, yangi dorilarning rivojlanishi) haqida ma'lumotlar keltirilgan. Ushbu tadqiqotning asosiy maqsadi leykotsit adgeziyasi va migratsiyasidagi patologiyalarning to'qimalarning normal fiziologiyasi va turli kasalliklarning patogenezidagi ahamiyatini tushunishni yanada chuqurlashtirish hamda yangi davolash strategiyalarini ishlab chiqish uchun ilmiy asos yaratishdir.
DOI: 10.15468/dl.zrznr9
title: Occurrence Download

description: A dataset containing 83 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Asia",
 "Country is China",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Vicia tetrasperma",
 "Year 1970-2000"
 ]
}

 The dataset includes 83 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007883-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.18238824
title: Achyrocline alata (Kunth) DC. In: Marchesi E., Mailhos A. & Bonifacino J.M. (Eds.). Flora Uruguaya: Flora Vascular del Uruguay. ISSN 3121-2050, V. 1.0.

description: Citable snapshot. Includes PDF and CSV of collections. Date: January 13th, 2026 at 16:41
DOI: 10.5281/zenodo.18238823
title: Achyrocline alata (Kunth) DC. In: Marchesi E., Mailhos A. & Bonifacino J.M. (Eds.). Flora Uruguaya: Flora Vascular del Uruguay. ISSN 3121-2050, V. 1.0.

description: Citable snapshot. Includes PDF and CSV of collections. Date: January 13th, 2026 at 16:41
DOI: 10.15468/dl.k3x7ce
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{"type":"and","predicates":[{"type":"equals","key":"GADM_LEVEL_2_GID","value":"USA.11.131_1","matchCase":false},{"type":"equals","key":"CLASS_KEY","value":"212","matchCase":false},{"type":"greaterThanOrEquals","key":"YEAR","value":"2015","matchCase":false},{"type":"not","predicate":{"type":"in","key":"BASIS_OF_RECORD","values":["MATERIAL_CITATION","MATERIAL_SAMPLE","PRESERVED_SPECIMEN"],"matchCase":false}},{"type":"lessThanOrEquals","key":"COORDINATE_UNCERTAINTY_IN_METERS","value":"10000","matchCase":false},{"type":"equals","key":"LICENSE","value":"CC BY 4.0","matchCase":false}]}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007884-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.upz6fa
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Malpaisomys insularis"
 ]
}

 The dataset includes 15 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007885-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.18238825
title: Achyrocline crassiuscula (Malme) Deble & Marchiori. In: Marchesi E., Mailhos A. & Bonifacino J.M. (Eds.). Flora Uruguaya: Flora Vascular del Uruguay. ISSN 3121-2050, V. 1.0.

description: Citable snapshot. Includes PDF and CSV of collections. Date: January 13th, 2026 at 16:41
DOI: 10.5281/zenodo.18238826
title: Achyrocline crassiuscula (Malme) Deble & Marchiori. In: Marchesi E., Mailhos A. & Bonifacino J.M. (Eds.). Flora Uruguaya: Flora Vascular del Uruguay. ISSN 3121-2050, V. 1.0.

description: Citable snapshot. Includes PDF and CSV of collections. Date: January 13th, 2026 at 16:41
DOI: 10.15468/dl.ervs7d
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Lepidophyma inagoi"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007886-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.18238833
title: Achyrocline flaccida (Weinm.) DC. In: Marchesi E., Mailhos A. & Bonifacino J.M. (Eds.). Flora Uruguaya: Flora Vascular del Uruguay. ISSN 3121-2050, V. 1.0.

description: Citable snapshot. Includes PDF and CSV of collections. Date: January 13th, 2026 at 16:41
DOI: 10.5281/zenodo.18238834
title: Achyrocline flaccida (Weinm.) DC. In: Marchesi E., Mailhos A. & Bonifacino J.M. (Eds.). Flora Uruguaya: Flora Vascular del Uruguay. ISSN 3121-2050, V. 1.0.

description: Citable snapshot. Includes PDF and CSV of collections. Date: January 13th, 2026 at 16:41
DOI: 10.15468/dl.dxxz3b
title: Occurrence Download

description: A dataset containing 9 species occurrences available in GBIF matching the query:
{"type":"and","predicates":[{"type":"equals","key":"GADM_LEVEL_2_GID","value":"USA.11.132_1","matchCase":false},{"type":"equals","key":"CLASS_KEY","value":"212","matchCase":false},{"type":"greaterThanOrEquals","key":"YEAR","value":"2015","matchCase":false},{"type":"not","predicate":{"type":"in","key":"BASIS_OF_RECORD","values":["MATERIAL_CITATION","MATERIAL_SAMPLE","PRESERVED_SPECIMEN"],"matchCase":false}},{"type":"lessThanOrEquals","key":"COORDINATE_UNCERTAINTY_IN_METERS","value":"10000","matchCase":false},{"type":"equals","key":"LICENSE","value":"CC BY 4.0","matchCase":false}]}

 The dataset includes 9 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007887-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.14557393
title: Localized plant species-specific BVOC emission rate library

description: Localized plant species-specific BVOC emission rate library was established based on our and reported local field measurements, a statistical approach of classifying more detailed emission categories and assigning the representative emission rates used in BVOC emission inventory was developed. The developed library included the isoprene, monoterpene, and sesquiterpene emission rates for 599 native plant species. Critically, different level of reliability was given to each emission rate according to the measurement technique. This library will be significant for improving the accuracy of local biogenic emission inventory and furtherly the modeling of air quality.
DOI: 10.5281/zenodo.14557394
title: Localized plant species-specific BVOC emission rate library

description: Localized plant species-specific BVOC emission rate library was established based on our and reported local field measurements, a statistical approach of classifying more detailed emission categories and assigning the representative emission rates used in BVOC emission inventory was developed. The developed library included the isoprene, monoterpene, and sesquiterpene emission rates for 599 native plant species. Critically, different level of reliability was given to each emission rate according to the measurement technique. This library will be significant for improving the accuracy of local biogenic emission inventory and furtherly the modeling of air quality.
DOI: 10.5281/zenodo.18238830
title: IMMUNOTROMBOZ VA MIKROTOMIRLAR ANATOMIYASI NETOZISNING TOMIR O'TKAZUVCHANLIGI VA TROMBOGENEZGA TA'SIRI

description: Immunotromboz – bu yallig‘lanish va qon ivishining o‘zaro bog‘liq holda ishlashi natijasida hosil bo‘ladigan patologik jarayon bo‘lib, mikrotomirlar darajasida rivojlanadigan asosiy klinik hodisalardan biridir. Ushbu jarayonda neytrofillarning o‘ziga xos o‘limi – NETozis (neytrofil ekstrasellyulyar tuzoqlari) muhim rol o‘ynaydi. NETozis jarayonida faollashgan neytrofillarning yadrolari parchalanishi va sitoplazmatik granulalaridan chiqqan DNK tolalari, gistonlar va mikrobitsid oqsillarning (MPO, neytrofil elastaza va boshqalar) ekstrasellyulyar tarmoqqa (NET) chiqishi kuzatiladi. Ushbu tarmoqlar dastlab patogenlarni ushlab qolish va yo‘q qilish vazifasini bajaradi. Biroq, patologik sharoitda, ayniqsa tomir devori (endoteliy) shikastlangan yoki yallig‘langan joyda, NETlar tomir o‘tkazuvchanligini va qon ivish kaskadini chuqur modulyatsiya qiladi. Maqolaning maqsadi 2015-2025-yillar oralig‘ida o‘tkazilgan ilmiy tadqiqotlar asosida NETozisning mikrotomirlar anatomiyasi va funksiyasiga, shuningdek, uning tromb hosil bo‘lishi (trombogenez) jarayonlariga ta’sirini tizimli tahli qilishdan iborat. Tadqiqotlar shuni ko‘rsatadiki, NETlar endotelial bo‘shliqlarning kengayishiga sabab bo‘lib, plazma oqsillari va qon hujayralarining tomir devoridan chiqib ketishini osonlashtiradi, bu esa mikrotomirlarda to‘qimalarning shishishi va ishemiyasiga olib keladi. Bundan tashqari, NET tarmoqlarining o‘zida joylashgan sitokitik oqsillar endotelial hujayralarga toksik ta’sir ko‘rsatadi va ularning apoptozini rag‘batlantiradi. Trombogenez nuqtai nazaridan, NETlar IV faktor va XII faktor kabi plazma oqsillarini faollashtirib, kontakt tizimi orqali qon ivishini boshlaydi. Ular, shuningdek, trombotsitlarning adgeziyasi va agregatsiyasini kuchaytiradi, shu bilan birga plazminogenning faollashishini ingibirlaydi, natijada fibrinolitik tizimning susayishi va barqaror tromb massalarining shakllanishi kuzatiladi. Ushbu maqola NETozisning mikrosirkulyator tomir yotog‘i va uning o‘tkazuvchanligiga ta’sir etadigan anatomik-morfologik o‘zgarishlar, shu jumladan bazal membrananing buzilishi, perisit funksiyasining yetishmovchiligi va kapillyar kanallarning torayishi kabi jihatlarni yoritadi. Xulosa qilib aytganda, NETozis tomir o‘tkazuvchanligi va trombogenezning asosiy tashkilotchisi sifatida nafaqat septik shok, qondiabetik angiopatiya, o‘tkir respirator distress sindromi (ARDS), COVID-19 bilan bog‘liq asoratlar, balki trombotik mikroangiopatiya va ko‘plab autoimmun kasalliklarning patogenezida ham muhim ahamiyatga ega. Ushbu jarayonlarni maqsadli nazorat qilish yangi terapevtik strategiyalarni ishlab chiqishga yo‘l ochishi mumkin.
DOI: 10.5281/zenodo.18238829
title: IMMUNOTROMBOZ VA MIKROTOMIRLAR ANATOMIYASI NETOZISNING TOMIR O'TKAZUVCHANLIGI VA TROMBOGENEZGA TA'SIRI

description: Immunotromboz – bu yallig‘lanish va qon ivishining o‘zaro bog‘liq holda ishlashi natijasida hosil bo‘ladigan patologik jarayon bo‘lib, mikrotomirlar darajasida rivojlanadigan asosiy klinik hodisalardan biridir. Ushbu jarayonda neytrofillarning o‘ziga xos o‘limi – NETozis (neytrofil ekstrasellyulyar tuzoqlari) muhim rol o‘ynaydi. NETozis jarayonida faollashgan neytrofillarning yadrolari parchalanishi va sitoplazmatik granulalaridan chiqqan DNK tolalari, gistonlar va mikrobitsid oqsillarning (MPO, neytrofil elastaza va boshqalar) ekstrasellyulyar tarmoqqa (NET) chiqishi kuzatiladi. Ushbu tarmoqlar dastlab patogenlarni ushlab qolish va yo‘q qilish vazifasini bajaradi. Biroq, patologik sharoitda, ayniqsa tomir devori (endoteliy) shikastlangan yoki yallig‘langan joyda, NETlar tomir o‘tkazuvchanligini va qon ivish kaskadini chuqur modulyatsiya qiladi. Maqolaning maqsadi 2015-2025-yillar oralig‘ida o‘tkazilgan ilmiy tadqiqotlar asosida NETozisning mikrotomirlar anatomiyasi va funksiyasiga, shuningdek, uning tromb hosil bo‘lishi (trombogenez) jarayonlariga ta’sirini tizimli tahli qilishdan iborat. Tadqiqotlar shuni ko‘rsatadiki, NETlar endotelial bo‘shliqlarning kengayishiga sabab bo‘lib, plazma oqsillari va qon hujayralarining tomir devoridan chiqib ketishini osonlashtiradi, bu esa mikrotomirlarda to‘qimalarning shishishi va ishemiyasiga olib keladi. Bundan tashqari, NET tarmoqlarining o‘zida joylashgan sitokitik oqsillar endotelial hujayralarga toksik ta’sir ko‘rsatadi va ularning apoptozini rag‘batlantiradi. Trombogenez nuqtai nazaridan, NETlar IV faktor va XII faktor kabi plazma oqsillarini faollashtirib, kontakt tizimi orqali qon ivishini boshlaydi. Ular, shuningdek, trombotsitlarning adgeziyasi va agregatsiyasini kuchaytiradi, shu bilan birga plazminogenning faollashishini ingibirlaydi, natijada fibrinolitik tizimning susayishi va barqaror tromb massalarining shakllanishi kuzatiladi. Ushbu maqola NETozisning mikrosirkulyator tomir yotog‘i va uning o‘tkazuvchanligiga ta’sir etadigan anatomik-morfologik o‘zgarishlar, shu jumladan bazal membrananing buzilishi, perisit funksiyasining yetishmovchiligi va kapillyar kanallarning torayishi kabi jihatlarni yoritadi. Xulosa qilib aytganda, NETozis tomir o‘tkazuvchanligi va trombogenezning asosiy tashkilotchisi sifatida nafaqat septik shok, qondiabetik angiopatiya, o‘tkir respirator distress sindromi (ARDS), COVID-19 bilan bog‘liq asoratlar, balki trombotik mikroangiopatiya va ko‘plab autoimmun kasalliklarning patogenezida ham muhim ahamiyatga ega. Ushbu jarayonlarni maqsadli nazorat qilish yangi terapevtik strategiyalarni ishlab chiqishga yo‘l ochishi mumkin.
DOI: 10.15468/dl.femsqg
title: Occurrence Download

description: A dataset containing 822433 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "not" : {
 "PublishingCountry" : [
 "is Norway"
 ]
 }
 },
 "Country is Svalbard and Jan Mayen",
 {
 "or" : [
 "CoordinateUncertaintyInMeters is null",
 "CoordinateUncertaintyInMeters is less than or equal to 10000"
 ]
 },
 {
 "not" : {
 "DatasetKey" : [
 "is EBCC Atlas of European Breeding Birds"
 ]
 }
 },
 {
 "not" : {
 "DatasetKey" : [
 "is (Table 3) Relative abundance of Azolla spp., peridinoid dinocysts, gonyaulacoid dinocysts, non-saccate pollen, bisaccate pollen and spores from the Lomonosov Ridge during different Arctic coring expeditions"
 ]
 }
 }
 ]
}

 The dataset includes 822433 records from 839 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007849-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2e9rqg
title: Occurrence Download

description: A dataset containing 503639 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Rhinella marina, Lithobates catesbeianus, Polypedates leucomystax, Andrias davidianus, Xenopus laevis, Rhinella diptycha, Kaloula pulchra, Anaxyrus terrestris, Anaxyrus debilis, Osteopilus septentrionalis, Duttaphrynus melanostictus, Bufo bufo, Bufotes viridis, Eleutherodactylus coqui, Anaxyrus cognatus, Rhaebo guttatus, Anaxyrus punctatus, Anaxyrus quercicus, Anaxyrus speciosus, Eleutherodactylus johnstonei, Eleutherodactylus planirostris, Bufo ailaoanus, Bufo aspinius, Bufo bankorensis, Bufo cryptotympanicus, Bufo eichwaldi, Bufo luchunnicus, Bufo menglianus, Bufo pageoti, Bufo spinosus, Bufo stejnegeri, Bufo tuberculatus, Bufo tuberospinius, Bufo verrucosissimus, Osteopilus crucialis, Osteopilus dominicensis, Osteopilus marianae, Osteopilus ocellatus, Osteopilus pulchrilineatus, Osteopilus vastus, Osteopilus wilderi, Lithobates clamitans, Lithobates grylio, Lithobates heckscheri, Lithobates okaloosae, Lithobates septentrionalis, Lithobates virgatipes, Incilius valliceps)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is Fossil"
 ]
 }
 },
 "Year is greater than or equal to 2000",
 "CoordinateUncertaintyInMeters is less than 1000"
 ]
}

 The dataset includes 503639 records from 892 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007874-260108223611665/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15277283
title: Survey design: GitHub Copilot in GovTech
DOI: 10.5281/zenodo.15277282
title: Survey design: GitHub Copilot in GovTech
DOI: 10.5281/zenodo.15277339
title: Supplementary Materials for "Tracking SARS-CoV-2 genomic variants in wastewater sequencing data with LolliPop".
DOI: 10.5281/zenodo.10987169
title: Survey design: GitHub Copilot in NAV
DOI: 10.5281/zenodo.10987170
title: Survey design: GitHub Copilot in NAV
DOI: 10.5281/zenodo.15448387
title: Online behavior patterns in teachers

description: The dataset contains 10 sociodemographic descriptive variables and a set of 11 psychometric measures about online behavior, problematic buying behavior, online vulnerability, anxiety, and depression. The answers correspond to a sample of 1043 teachers.
DOI: 10.5281/zenodo.15448388
title: Online behavior patterns in teachers

description: The dataset contains 10 sociodemographic descriptive variables and a set of 11 psychometric measures about online behavior, problematic buying behavior, online vulnerability, anxiety, and depression. The answers correspond to a sample of 1043 teachers.
DOI: 10.5281/zenodo.15367749
title: Phylogenetically compressed 661k collection (.tar.xz) - High quality genomes

description: Phylogenetically compressed 661k collection (high-quality genomes only) with XZ as a low-level compressor. More information can be found on https://brinda.eu/mof/.

Batching is done with the GTDBk classification from: https://bakrep.computational.bio/search.

Criteria for genomes:





Size between 100 kbp and 15 Mbp




Fewer than 2,000 contigs




N50 greater than 5,000




Completeness ≥ 90%




Contamination ≤ 5%



These criteria yield 631,561 assemblies, compared to the 639,981 reported in the original 661k paper.

Due to file count limitations on Zenodo, the 284 batches are grouped into 99 tar files. Metadata is provided to indicate which tar file contains each batch.

Citation:



Břinda, K., Lima, L., Pignotti, S. et al. Efficient and robust search of microbial genomes via phylogenetic compression. Nat Methods 22, 692–697 (2025). https://doi.org/10.1038/s41592-025-02625-2

Grace A. Blackwell et al., “Exploring Bacterial Diversity via a Curated and Searchable Snapshot of Archived DNA Sequences,” PLOS Biology 19, no. 11 (November 9, 2021): e3001421, https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001421.

Linda Fenske et al., “BakRep – a Searchable Large-Scale Web Repository for Bacterial Genomes, Characterizations and Metadata,” Microbial Genomics 10, no. 10 (2024): 001305, https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001305.
DOI: 10.5281/zenodo.15367750
title: Phylogenetically compressed 661k collection (.tar.xz) - High quality genomes

description: Phylogenetically compressed 661k collection (high-quality genomes only) with XZ as a low-level compressor. More information can be found on https://brinda.eu/mof/.

Batching is done with the GTDBk classification from: https://bakrep.computational.bio/search.

Criteria for genomes:





Size between 100 kbp and 15 Mbp




Fewer than 2,000 contigs




N50 greater than 5,000




Completeness ≥ 90%




Contamination ≤ 5%



These criteria yield 631,561 assemblies, compared to the 639,981 reported in the original 661k paper.

Due to file count limitations on Zenodo, the 284 batches are grouped into 99 tar files. Metadata is provided to indicate which tar file contains each batch.

Citation:



Břinda, K., Lima, L., Pignotti, S. et al. Efficient and robust search of microbial genomes via phylogenetic compression. Nat Methods 22, 692–697 (2025). https://doi.org/10.1038/s41592-025-02625-2

Grace A. Blackwell et al., “Exploring Bacterial Diversity via a Curated and Searchable Snapshot of Archived DNA Sequences,” PLOS Biology 19, no. 11 (November 9, 2021): e3001421, https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001421.

Linda Fenske et al., “BakRep – a Searchable Large-Scale Web Repository for Bacterial Genomes, Characterizations and Metadata,” Microbial Genomics 10, no. 10 (2024): 001305, https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001305.
DOI: 10.6084/m9.figshare.29092295
title: Supplementary Material for: Cryobiopsy-based Tri-modality sampling using an ultrathin bronchoscope for the diagnosis of peripheral lung lesions: a prospective observational study

description: Introduction: Transbronchial lung biopsy using radial probe endobronchial ultrasound (RP-EBUS) is an important modality for diagnosing peripheral lung lesions (PLLs), but unmet needs remain. This study evaluates the diagnostic yields and safety of cryobiopsy-based Tri-modality sampling using an ultrathin bronchoscope (UTB) with RP-EBUS for diagnosing PLLs.
Methods: This prospective observational pilot study was conducted from November 2023 to January 2024, and included patients with PLLs ≤ 30 mm in diameter. Bronchoscopic procedures were performed using a UTB guided by RP-EBUS. Tri-modality sampling was sequentially conducted using cryobiopsy, forceps biopsy, and transbronchial needle aspiration (TBNA). The primary outcome was cumulative diagnostic yield, secondary outcomes included the diagnostic yield of each modality and adverse events.
Results: Fifty patients with PLLs, with a median diameter of 20.3 mm, were included. A bronchus sign was present in 66% of cases, and lesions were invisible on RP-EBUS in three instances. The overall cumulative diagnostic yield was 78%. The diagnostic yields were 78.7%, 66.7 %, and 54.2% for cryobiopsy, forceps biopsy, and TBNA, respectively. Adding forceps biopsy to cryobiopsy improved the diagnostic yield by 4%, from 74% to 78%, and increased sensitivity for malignancy by 5.1%, from 84.6% to 89.7%. TBNA did not provide any additional improvement in diagnostic yield or sensitivity to malignancy. Bleeding occurred in 68% of the patients, with 28% experiencing grade 3 bleeding, all managed without life-threatening complications. 
Conclusion: Cryobiopsy-based combination sampling modality using UTB is feasible and safe for diagnosing PLLs, offering high diagnostic yield and improved sensitivity for malignancy.