cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25549/uschist-c104-289724
title: USC trustee delegation trip to China, 2006-05-22: Beijing

description: 131 photographs of the University of Southern California trustee delegation in Beijing, China, 2006 May 22. Meeting with dignitary, the Forbidden City, The St. Regis hotel interiors, dinner, breakfast, meetings, Beijing exterior views, presentation of International Alumna of the 20th Century award to 101-year old Jieqiong Lei, Great Wall of China. Trustees and protocol contingent pictured include: Betsy Amster, David Ayón, Charles Bolden, Ronnie Chan, Dennis Cornell, Lillian Doeng, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Herb Klein, Lei Jieqiong, Liu Erh Fei, Monica Lozano, Max Nikias, Niki Nikias, Edward Roski, Kathryn Sample, Steven Sample, Sharon Schoen, William Schoen, Mark Stevens, Edward T..., Gwendolyn Watt, Raymond Watt, Anne Westfall, Norene Zapanta, Zhang Xin. Photography by Jon Weiner (94 series), Baizhu (101 series), Elizabeth Garrett (102 series), Charles Bolden (107 series), Stanley Gold (110 series)
DOI: 10.25549/uschist-c104-289731
title: USC trustee delegation trip to China, 2006-05-22: Beijing

description: 131 photographs of the University of Southern California trustee delegation in Beijing, China, 2006 May 22. Meeting with dignitary, the Forbidden City, The St. Regis hotel interiors, dinner, breakfast, meetings, Beijing exterior views, presentation of International Alumna of the 20th Century award to 101-year old Jieqiong Lei, Great Wall of China. Trustees and protocol contingent pictured include: Betsy Amster, David Ayón, Charles Bolden, Ronnie Chan, Dennis Cornell, Lillian Doeng, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Herb Klein, Lei Jieqiong, Liu Erh Fei, Monica Lozano, Max Nikias, Niki Nikias, Edward Roski, Kathryn Sample, Steven Sample, Sharon Schoen, William Schoen, Mark Stevens, Edward T..., Gwendolyn Watt, Raymond Watt, Anne Westfall, Norene Zapanta, Zhang Xin. Photography by Jon Weiner (94 series), Baizhu (101 series), Elizabeth Garrett (102 series), Charles Bolden (107 series), Stanley Gold (110 series)
DOI: 10.25549/uschist-c104-286921
title: USC trustee delegation trip to China, 2006-05-23: Beijing & Shanghai

description: 98 photographs of the University of Southern California trustee delegation in Beijing and Shanghai, China, 2006 May 23. Tiananmen Square, concert and dance performance at the Great Hall of the People, dignitary meeting with the mayor of Beijing, airport, Shanghai school, Shanghai exteriors. Trustees and protocol contingent pictured include: Marie Allen, William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Zhou Bing, Charles Bolden, George Boone, MaryLou Boone, Gail Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Ronnie Chan, Yang-Ho Cho, Dennis Cornell, Barbara Currie, Malcolm Currie, Dana Dornsife, David Dornsife, Dennis Dougherty, Daniel Epstein, Phyllis Epstein, Barry Glassner, Ilene Gold, Stanley Gold, Martha Harris, Ann Hill, Ghada Irani, Ray Irani, Herb Klein, Alan Kreditor, Dolores Kusmiersky, John Kusmiersky, Kenneth Leventhal, Elaine Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy neely, Max Nikias, Niki Nikias, Tosh Ogasawara, Yukiko Ogasawara, Kristoffer Popovich, Jane Popovich, Frank Price, Katherine Price, Blake Quinn, Terri Quinn, Kim Rollo, Robert Rollo, Edward Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, William Schoen, William Siart, Laura Siart, Loretta Smith, Robert Smith, Mark Stevens, Valerie Sugar, David Tappan, Gwendolyn Watt, Raymond Watt, Anne Westfall, and Stan Westfall. Photography by Jon Weiner (94 series), Johansen Krause (95 series), Baizhu (101 series), Elizabeth Garrett (102 series), William Allen (103 series), Charles Bolden (108-109 series), Stanley Gold (110 series).
DOI: 10.25549/uschist-c104-286926
title: USC trustee delegation trip to China, 2006-05-23: Beijing & Shanghai

description: 98 photographs of the University of Southern California trustee delegation in Beijing and Shanghai, China, 2006 May 23. Tiananmen Square, concert and dance performance at the Great Hall of the People, dignitary meeting with the mayor of Beijing, airport, Shanghai school, Shanghai exteriors. Trustees and protocol contingent pictured include: Marie Allen, William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Zhou Bing, Charles Bolden, George Boone, MaryLou Boone, Gail Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Ronnie Chan, Yang-Ho Cho, Dennis Cornell, Barbara Currie, Malcolm Currie, Dana Dornsife, David Dornsife, Dennis Dougherty, Daniel Epstein, Phyllis Epstein, Barry Glassner, Ilene Gold, Stanley Gold, Martha Harris, Ann Hill, Ghada Irani, Ray Irani, Herb Klein, Alan Kreditor, Dolores Kusmiersky, John Kusmiersky, Kenneth Leventhal, Elaine Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy neely, Max Nikias, Niki Nikias, Tosh Ogasawara, Yukiko Ogasawara, Kristoffer Popovich, Jane Popovich, Frank Price, Katherine Price, Blake Quinn, Terri Quinn, Kim Rollo, Robert Rollo, Edward Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, William Schoen, William Siart, Laura Siart, Loretta Smith, Robert Smith, Mark Stevens, Valerie Sugar, David Tappan, Gwendolyn Watt, Raymond Watt, Anne Westfall, and Stan Westfall. Photography by Jon Weiner (94 series), Johansen Krause (95 series), Baizhu (101 series), Elizabeth Garrett (102 series), William Allen (103 series), Charles Bolden (108-109 series), Stanley Gold (110 series).
DOI: 10.15468/dl.jykdxn
title: Occurrence Download

description: A dataset containing 639 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-104.76717 43.01722,-102.91607 43.01722,-102.91607 44.99329,-104.76717 44.99329,-104.76717 43.01722))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Cirsium arvense"
 ]
}

 The dataset includes 639 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030494-260208012135463/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/uschist-c104-286295
title: USC trustee delegation trip to China, 2006-05-24: Shanghai

description: 110 photographs of the University of Southern California trustee delegation in Shanghai, China, 2006 May 24. Four Seasons hotel, Shanghai exterior day and night views, dignitary meeting, jazz and dancing at Peace Hotel, visit to ChinaVest headquarters. Trustees and protocol contingent pictured include: Marie Allen, William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Judith Blumenthal, Charles Bolden, George Boone, MaryLou Boone, Gail Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Alex Cappello, Ronnie Chan, Yang-Ho Cho, Dennis Cornell, Barbara Currie, Malcolm Currie, Marybeth Cutietta, Rob Cutietta, Dana Dornsife, David Dornsife, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Martha Harris, Ray Irani, Herb Klein,Dolores Kusmiersky, John Kusmiersky, Reggie Lathan, Kenneth Leventhal, Elaine Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy Neely, Max Nikias, Niki Nikias, Tosh Ogasawara, Yukiko Ogasawara, Kristoffer Popovich, Jane Popovich, Frank Price, Katherine Price, Blake Quinn, Terri Quinn, Kim Rollo, Robert Rollo, Edward Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, William Schoen, William Siart, Laura Siart, Loretta Smith, Robert Smith, Andrea Sonnenberg, Glenn Sonnenberg, Mark Stevens, Valerie Sugar, David Tappan, Gwendolyn Watt, Raymond Watt, Anne Westfall, and Stan Westfall, Norene Murray Zapanta. Photography by Jon Weiner (94 series), Charles Bolden (109 series).
DOI: 10.25549/uschist-c104-286297
title: USC trustee delegation trip to China, 2006-05-24: Shanghai

description: 110 photographs of the University of Southern California trustee delegation in Shanghai, China, 2006 May 24. Four Seasons hotel, Shanghai exterior day and night views, dignitary meeting, jazz and dancing at Peace Hotel, visit to ChinaVest headquarters. Trustees and protocol contingent pictured include: Marie Allen, William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Judith Blumenthal, Charles Bolden, George Boone, MaryLou Boone, Gail Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Alex Cappello, Ronnie Chan, Yang-Ho Cho, Dennis Cornell, Barbara Currie, Malcolm Currie, Marybeth Cutietta, Rob Cutietta, Dana Dornsife, David Dornsife, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Martha Harris, Ray Irani, Herb Klein,Dolores Kusmiersky, John Kusmiersky, Reggie Lathan, Kenneth Leventhal, Elaine Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy Neely, Max Nikias, Niki Nikias, Tosh Ogasawara, Yukiko Ogasawara, Kristoffer Popovich, Jane Popovich, Frank Price, Katherine Price, Blake Quinn, Terri Quinn, Kim Rollo, Robert Rollo, Edward Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, William Schoen, William Siart, Laura Siart, Loretta Smith, Robert Smith, Andrea Sonnenberg, Glenn Sonnenberg, Mark Stevens, Valerie Sugar, David Tappan, Gwendolyn Watt, Raymond Watt, Anne Westfall, and Stan Westfall, Norene Murray Zapanta. Photography by Jon Weiner (94 series), Charles Bolden (109 series).
DOI: 10.25549/uschist-c104-287090
title: USC trustee delegation trip to China, 2006-05-21: Beijing

description: 98 photographs of the University of Southern California trustee delegation in Beijing, China, 2006 May 21. Arrival at the hotel, buffet, reception at the U.S. Embassy residence (includes painting of George Bush) with Ambassador Clark T. Randt, Jr., meeting with dignitary in traditional Chinese building, tour of CCTV headquarters construction site. Trustees and protocol contingent pictured include: Marie Allen, William Allen, Betsy Amster, David Ayón, Gale Bensussen, Judith Blumenthal, Charles Bolden, George Boone, Gail Boskovich, Joseph Boskovich, Greg Brakovich, Sue Brakovich, Alex Cappello, Dennis Cornell, Barbara Currie, Malcolm Currie, Gail Ellis, James Ellis, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Ann Hill, Herb Klein, Elaine Leventhal, Kenneth Leventhal, Monica Lozano, Michael Mayne, Max Nikias, Tosh Ogasawara, Yukiko Ogasawara, Jane Hoffman Popovich, J. Kristoffer Popovich, Terri Quinn, Kim Rollo, Robert Rollo, Edward Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, William Schoen, Bill Siart, Laura Siart, Robert Smith, Mark Stevens, David Tappan, Stan Westfall, Norene Zapanta. Photography by Jon Weiner (94 series), Baizhu (100-101 series), Elizabeth Garrett (102 series), Charles Bolden (107 series), Stanley Gold (110 series).
DOI: 10.15468/dl.mx6zyd
title: Occurrence Download

description: A dataset containing 639 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-104.76717 43.01722,-102.91607 43.01722,-102.91607 44.99329,-104.76717 44.99329,-104.76717 43.01722))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Cirsium arvense"
 ]
}

 The dataset includes 639 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030492-260208012135463/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/uschist-c104-287097
title: USC trustee delegation trip to China, 2006-05-21: Beijing

description: 98 photographs of the University of Southern California trustee delegation in Beijing, China, 2006 May 21. Arrival at the hotel, buffet, reception at the U.S. Embassy residence (includes painting of George Bush) with Ambassador Clark T. Randt, Jr., meeting with dignitary in traditional Chinese building, tour of CCTV headquarters construction site. Trustees and protocol contingent pictured include: Marie Allen, William Allen, Betsy Amster, David Ayón, Gale Bensussen, Judith Blumenthal, Charles Bolden, George Boone, Gail Boskovich, Joseph Boskovich, Greg Brakovich, Sue Brakovich, Alex Cappello, Dennis Cornell, Barbara Currie, Malcolm Currie, Gail Ellis, James Ellis, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Ann Hill, Herb Klein, Elaine Leventhal, Kenneth Leventhal, Monica Lozano, Michael Mayne, Max Nikias, Tosh Ogasawara, Yukiko Ogasawara, Jane Hoffman Popovich, J. Kristoffer Popovich, Terri Quinn, Kim Rollo, Robert Rollo, Edward Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, William Schoen, Bill Siart, Laura Siart, Robert Smith, Mark Stevens, David Tappan, Stan Westfall, Norene Zapanta. Photography by Jon Weiner (94 series), Baizhu (100-101 series), Elizabeth Garrett (102 series), Charles Bolden (107 series), Stanley Gold (110 series).
DOI: 10.25549/uschist-c104-289735
title: USC trustee delegation trip to China, 2006-05-22: Beijing

description: 131 photographs of the University of Southern California trustee delegation in Beijing, China, 2006 May 22. Meeting with dignitary, the Forbidden City, The St. Regis hotel interiors, dinner, breakfast, meetings, Beijing exterior views, presentation of International Alumna of the 20th Century award to 101-year old Jieqiong Lei, Great Wall of China. Trustees and protocol contingent pictured include: Betsy Amster, David Ayón, Charles Bolden, Ronnie Chan, Dennis Cornell, Lillian Doeng, Daniel Epstein, Phyllis Epstein, Elizabeth Garrett, Barry Glassner, Ilene Gold, Stanley Gold, Herb Klein, Lei Jieqiong, Liu Erh Fei, Monica Lozano, Max Nikias, Niki Nikias, Edward Roski, Kathryn Sample, Steven Sample, Sharon Schoen, William Schoen, Mark Stevens, Edward T..., Gwendolyn Watt, Raymond Watt, Anne Westfall, Norene Zapanta, Zhang Xin. Photography by Jon Weiner (94 series), Baizhu (101 series), Elizabeth Garrett (102 series), Charles Bolden (107 series), Stanley Gold (110 series)
DOI: 10.25549/uschist-c104-287687
title: USC trustee delegation trip to China, 2006-05-27: Hong Kong floating restaurant

description: 65 photographs of the University of Southern California trustee delegation in Hong Kong, China, 2006 May 27. Boat trip to the Jumbo Floating Restaurant and lunch. Trustees, administration and their hosts pictured include: William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Judith Blumenthal, Charles Bolden, Gregory Brakovich, Susan Brackovich, Yang-Ho Cho, Marybeth Cutietta, Rob Cutietta, Dana Dornsife, David Dornsife, Elizabeth Garrett, Barry Glassner, Martha Harris, Herb Klein, Alan Kreditor, Elaine Leventhal, Kenneth Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy Neely, Max Nikias, Niki Nikias, Frank Price, Bruce Ramer, Kim Rollo, Robert Rollo, Barbara Rossier, Roger Rossier, Sharon Schoen, William Schoen, Robert Smith, Jeffrey Smulyen, Andrea Sonnenberg, Glenn Sonnenberg, David Tappan, Gwendolyn Watt, Raymond Watt, Norene Zapanta. Photography by Jon Weiner (94 series), William Allen (103 series), Baizhu (101 series), Charles Bolden (104, 106 series).
DOI: 10.25549/uschist-c104-287702
title: USC trustee delegation trip to China, 2006-05-27: Hong Kong floating restaurant

description: 65 photographs of the University of Southern California trustee delegation in Hong Kong, China, 2006 May 27. Boat trip to the Jumbo Floating Restaurant and lunch. Trustees, administration and their hosts pictured include: William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Judith Blumenthal, Charles Bolden, Gregory Brakovich, Susan Brackovich, Yang-Ho Cho, Marybeth Cutietta, Rob Cutietta, Dana Dornsife, David Dornsife, Elizabeth Garrett, Barry Glassner, Martha Harris, Herb Klein, Alan Kreditor, Elaine Leventhal, Kenneth Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy Neely, Max Nikias, Niki Nikias, Frank Price, Bruce Ramer, Kim Rollo, Robert Rollo, Barbara Rossier, Roger Rossier, Sharon Schoen, William Schoen, Robert Smith, Jeffrey Smulyen, Andrea Sonnenberg, Glenn Sonnenberg, David Tappan, Gwendolyn Watt, Raymond Watt, Norene Zapanta. Photography by Jon Weiner (94 series), William Allen (103 series), Baizhu (101 series), Charles Bolden (104, 106 series).
DOI: 10.15468/dl.8gjdks
title: Occurrence Download

description: A dataset containing 49192 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Vascular Plants Collection of Sagamihara City Museum"
 ]
}

 The dataset includes 49192 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030491-260208012135463/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/uschist-c104-287714
title: USC trustee delegation trip to China, 2006-05-27: Hong Kong floating restaurant

description: 65 photographs of the University of Southern California trustee delegation in Hong Kong, China, 2006 May 27. Boat trip to the Jumbo Floating Restaurant and lunch. Trustees, administration and their hosts pictured include: William Allen, Betsy Amster, David Ayón, Gale Bensussen, Jane Bensussen, Judith Blumenthal, Charles Bolden, Gregory Brakovich, Susan Brackovich, Yang-Ho Cho, Marybeth Cutietta, Rob Cutietta, Dana Dornsife, David Dornsife, Elizabeth Garrett, Barry Glassner, Martha Harris, Herb Klein, Alan Kreditor, Elaine Leventhal, Kenneth Leventhal, Monica Lozano, Morgan Lyons, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy Neely, Max Nikias, Niki Nikias, Frank Price, Bruce Ramer, Kim Rollo, Robert Rollo, Barbara Rossier, Roger Rossier, Sharon Schoen, William Schoen, Robert Smith, Jeffrey Smulyen, Andrea Sonnenberg, Glenn Sonnenberg, David Tappan, Gwendolyn Watt, Raymond Watt, Norene Zapanta. Photography by Jon Weiner (94 series), William Allen (103 series), Baizhu (101 series), Charles Bolden (104, 106 series).
DOI: 10.25549/uschist-c104-289792
title: USC trustee delegation trip to China, 2006-05-25: Shanghai small dinner

description: 51 photographs of the University of Southern California trustee delegation in Shanghai, China, 2006 May 25. Small dinner. Trustees and hosts pictured include: Marie Allen, William Allen, Gale Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Bill Gu, Reggie Lathan, Blake Quinn, Terri Quinn, Jeffrey Smulyan, Mark Stevens, John Van Fleet, Norene Zapenta.
DOI: 10.15468/dl.74dqx7
title: Occurrence Download

description: A dataset containing 620523 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is North America",
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Accipitridae"
 ]
}

 The dataset includes 620523 records from 133 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0030455-260208012135463/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/uschist-c104-289793
title: USC trustee delegation trip to China, 2006-05-25: Shanghai small dinner

description: 51 photographs of the University of Southern California trustee delegation in Shanghai, China, 2006 May 25. Small dinner. Trustees and hosts pictured include: Marie Allen, William Allen, Gale Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Bill Gu, Reggie Lathan, Blake Quinn, Terri Quinn, Jeffrey Smulyan, Mark Stevens, John Van Fleet, Norene Zapenta.
DOI: 10.25549/uschist-c104-288134
title: USC trustee delegation trip to China, 2006-05-27: Hong Kong Indian restaurant

description: 195 photographs of the University of Southern California trustee delegation in Hong Kong, China, 2006 May 27. Dinner in an Indian restaurant. Trustees, administration and their hosts pictured include: Marie Allen, William Allen, David Ayón, Gale Bensussen, Jane Bensussen, Judith Blumenthal, Charles Bolden, George Boone, MaryLou Boone, Gail Boskovich, Joseph Boskovich, Gregory Brakovich, Susan Brakovich, Alexander Cappello, Yang-Ho Cho, Dana Dornsife, David Dornsife, Dennis Dougherty, Daniel Epstein, Phyllis Epstein, Ilene Gold, Stanley Gold, Marsha Harris, Ann Hill, Herb Klein, Alan Kreditor, Dolores Kusmiersky, John Kusmiersky, Catherine Leung, Elaine Leventhal, Kenneth Leventhal, Morgan Lyons, Monica Lozano, Michael Mayne, Kathleen McCarthy, Jerry Neely, Nancy Neely, Max Nikias, Niki Nikias, Jane Popovich, Kristoffer Popovich, Frank Price, Katherine Price, Blake Quinn, Terri Quinn, Bruce Ramer, Edward Roski, Gayle Roski, Barbara Rossier, Roger Rossier, Kathryn Sample, Steven Sample, Sharon Shoen, William Schoen, Laura Siart, William Siart, Loretta Smith, Robert Smith, Jeffrey Smulyan, Andrea Sonnenberg, Glenn Sonnenberg, Valerie Sugar, David Tappan, Gwendolyn Watt, Raymond Watt, Douglas Yu, Norene Zapanta. Photography by Jon Weiner (62), Charles Bolden (104 series).
DOI: 10.25376/hra.31374127
title: 41467_2024_54934_MOESM8_ESM.xlsx

description: Clade 2.3.4.4b H5N1 is causing an unprecedented outbreak in dairy cows in the United States. To understand if recent H5N1 viruses are changing their receptor use, we screened recombinant hemagglutinin (HA) from historical and recent 2.3.4.4b H5N1 viruses for binding to distinct glycans bearing terminal sialic acids using a glycan microarray. We find that H5 from A/Texas/37/2024, an isolate from the dairy cow outbreak, has increased binding breadth to core glycans bearing terminal α2,3 sialic acids, the avian receptor, compared to historical and recent 2.3.4.4b H5N1 viruses. We do not observe any binding to α2,6 sialic acids, the receptor used by human seasonal influenza viruses. Using molecular dynamics and a cryo-EM structure of A/Texas/37/2024 H5, we show A/Texas/37/2024 H5 is more flexible within the receptor-binding site compared to a 2.3.4.4b H5 from 2022. We identify a single mutation outside of the receptor binding site, T199I, is responsible for increased binding breadth, as it increases receptor binding site flexibility. Together, these data show recent H5N1 viruses are evolving increased receptor binding breadth which could impact the host range and cell types infected with H5N1.
DOI: 10.25376/hra.31374127.v1
title: 41467_2024_54934_MOESM8_ESM.xlsx

description: Clade 2.3.4.4b H5N1 is causing an unprecedented outbreak in dairy cows in the United States. To understand if recent H5N1 viruses are changing their receptor use, we screened recombinant hemagglutinin (HA) from historical and recent 2.3.4.4b H5N1 viruses for binding to distinct glycans bearing terminal sialic acids using a glycan microarray. We find that H5 from A/Texas/37/2024, an isolate from the dairy cow outbreak, has increased binding breadth to core glycans bearing terminal α2,3 sialic acids, the avian receptor, compared to historical and recent 2.3.4.4b H5N1 viruses. We do not observe any binding to α2,6 sialic acids, the receptor used by human seasonal influenza viruses. Using molecular dynamics and a cryo-EM structure of A/Texas/37/2024 H5, we show A/Texas/37/2024 H5 is more flexible within the receptor-binding site compared to a 2.3.4.4b H5 from 2022. We identify a single mutation outside of the receptor binding site, T199I, is responsible for increased binding breadth, as it increases receptor binding site flexibility. Together, these data show recent H5N1 viruses are evolving increased receptor binding breadth which could impact the host range and cell types infected with H5N1.