cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.nmapa9
title: Occurrence Download

description: A dataset containing 428 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Mastofauna of the Bosque Protector Cerro Blanco during the dry season"
 ]
}

 The dataset includes 428 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0056573-240506114902167/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4ybfko
title: Occurrence Download

description: A dataset containing 44 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Belgium",
 "TaxonKey is Castor fiber Linnaeus, 1758"
 ]
}

 The dataset includes 44 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000131-190415153152247/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rde9ea
title: Occurrence Download

description: A dataset containing 449 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Aplocnemus alpestris Kiesenwetter, 1861, Carabus concolor Fabricius, 1792)",
 "Geometry POLYGON((-9.36035 44.71551,-10.59082 42.81152,-11.82129 36.80928,-7.69043 36.17336,-3.38379 35.88905,3.12012 38.13456,9.18457 38.20366,11.7334 37.16032,12.78809 34.74161,17.09473 33.79741, 23.15918 34.23451,30.10254 32.62087,33.2666 32.99024,35.72754 35.03,33.00293 35.53223,28.69629 35.17381,28.34473 36.31513,25.7959 37.50973,24.91699 39.90974,28.78418 40.6473,30.71777 41.17865,36.87012 45.213,38.18848 46.98025,38.71582 47.7541,39.94629 47.93107,40.20996 49.61071,37.0459 50.34546,35.72754 50.40152,33.79395 52.26816,31.68457 51.94426,31.4209 53.27835,32.56348 53.43572,30.98145 54.77535,30.89355 55.52863,28.43262 56.02295,27.20215 57.65716,27.37793 58.30949,27.90527 59.17593,29.66309 59.88894,27.6416 60.58697,31.50879 62.75473,28.78418 69.83962,30.89355 70.28912,29.22363 71.55274,21.75293 71.30079,14.28223 70.14036,11.38184 67.47492,4.87793 63.66576,-5.53711 71.52491,-10.63477 71.69129,-21.5332 67.74276,-28.56445 65.36684,-24.3457 61.35461,-9.36035 44.71551))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2021"
 ]
}

 The dataset includes 449 records from 23 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0156696-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nt6rve
title: Occurrence Download

description: A dataset containing 117600 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Hieracium bauhinii Schwägr. ex Schrank, 1822, Hordeum murinum L., Hordeum murinum L., Knautia godetii Reut., Luzula spicata (L.) DC.)",
 "Geometry POLYGON((-9.36035 44.71551,-10.59082 42.81152,-11.82129 36.80928,-7.69043 36.17336,-3.38379 35.88905,3.12012 38.13456,9.18457 38.20366,11.7334 37.16032,12.78809 34.74161,17.09473 33.79741, 23.15918 34.23451,30.10254 32.62087,33.2666 32.99024,35.72754 35.03,33.00293 35.53223,28.69629 35.17381,28.34473 36.31513,25.7959 37.50973,24.91699 39.90974,28.78418 40.6473,30.71777 41.17865,36.87012 45.213,38.18848 46.98025,38.71582 47.7541,39.94629 47.93107,40.20996 49.61071,37.0459 50.34546,35.72754 50.40152,33.79395 52.26816,31.68457 51.94426,31.4209 53.27835,32.56348 53.43572,30.98145 54.77535,30.89355 55.52863,28.43262 56.02295,27.20215 57.65716,27.37793 58.30949,27.90527 59.17593,29.66309 59.88894,27.6416 60.58697,31.50879 62.75473,28.78418 69.83962,30.89355 70.28912,29.22363 71.55274,21.75293 71.30079,14.28223 70.14036,11.38184 67.47492,4.87793 63.66576,-5.53711 71.52491,-10.63477 71.69129,-21.5332 67.74276,-28.56445 65.36684,-24.3457 61.35461,-9.36035 44.71551))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2021"
 ]
}

 The dataset includes 117600 records from 485 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0161759-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.zkgjj2
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hyperolius tuberilinguis Smith, 1849",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((15.6372 -28.0032,16.2782 -28.5786,20.509 -28.5786,20.509 -24.0715,15.4933 -24.0715,15.4945 -24.1257,16.0509 -25.6631,15.6372 -28.0032))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034418-250525065834625/datasets/export for details.
DOI: 10.15468/dl.f4wvf6
title: Occurrence Download

description: A dataset containing 2183 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is South America",
 "Country is Colombia",
 "Geometry POLYGON((-75.8624 1.91469,-75.6893 1.91469,-75.6893 2.10573,-75.8624 2.10573,-75.8624 1.91469))",
 "HasGeospatialIssue is false",
 "TaxonKey is Chordata",
 "Year 2000-2025"
 ]
}

 The dataset includes 2183 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034419-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4cpd2m
title: Occurrence Download

description: A dataset containing 85 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Material sample)"
 ]
 }
 },
 "TaxonKey is Taterillus pygargus (F.Cuvier, 1838)",
 "Continent is one of (Africa)",
 "Year is greater than or equal to 1900"
 ]
}

 The dataset includes 85 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034420-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.szfps8
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hyperolius tuberilinguis Smith, 1849",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((15.4784 -23.4046,15.4933 -24.0715,20.509 -24.0715,20.509 -24.0455,20.0952 -23.5072,19.9968 -23.017,19.7843 -22.5405,19.1988 -21.8785,18.5972 -21.5447,17.8859 -21.3993,17.1626 -21.4805,16.5332 -21.7596,16.1257 -22.0882,15.8234 -22.4662,15.606 -22.8993,15.4784 -23.4046))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034421-250525065834625/datasets/export for details.
DOI: 10.15468/dl.6z4r33
title: Occurrence Download

description: A dataset containing 5357 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Hybognathus nuchalis Agassiz, 1855"
 ]
}

 The dataset includes 5357 records from 61 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034422-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.74gv5u
title: Occurrence Download

description: A dataset containing 1787 species occurrences available in GBIF matching the query:
{
 "and" : [
 "CoordinateUncertaintyInMeters 0.0-2499.0",
 "OccurrenceStatus is Present",
 "TaxonKey is Speleomantes strinatii (Aellen, 1958)"
 ]
}

 The dataset includes 1787 records from 72 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034423-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ba4zpc
title: Occurrence Download

description: A dataset containing 46 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Costa Rica",
 "TaxonKey is Clitoria javitensis (Kunth) Benth."
 ]
}

 The dataset includes 46 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034425-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15615451
title: GL‑5.0: GR + Λ Deflection Baseline under Weak-Field Approximation

description: This dataset provides the control simulation for GL‑5, measuring gravitational deflection angles under pure GR and GR + Λ (Schwarzschild–de Sitter) metrics. The goal is to establish that Λ (cosmological constant) introduces no observable change in deflection angles at realistic lensing distances (50–500 kpc impact parameters). Both deflection curves (α_GR and α_Λ) were found to be numerically identical, confirming that curvature effects introduced by the Tejera-Ace Curvature Factor (CF) are not mimicking Λ contributions.

This run serves as the validated baseline geometry prior to CF injection and is part of the TASM GL-series under the Simulation Scrutiny Protocol.
DOI: 10.15468/dl.7852xc
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Material sample)"
 ]
 }
 },
 "TaxonKey is Taterillus tranieri Dobigny, Granjon, Aniskin, Ba & Volobouev, 2003",
 "Continent is one of (Africa)",
 "Year is greater than or equal to 1900"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034424-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.mpkm5y
title: Occurrence Download

description: A dataset containing 41 species occurrences available in GBIF matching the query:
{
 "and" : [
 "CoordinateUncertaintyInMeters 0.0-180.0",
 "GadmGid is USA.13_1",
 "Geometry POLYGON((-111.77704 41.97959,-111.20313 42.52003,-110.96878 44.70091,-112.51357 44.61961,-112.70966 42.3622,-111.77704 41.97959))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Oncorhynchus virginalis (Girard, 1856)"
 ]
}

 The dataset includes 41 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034430-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.w37khc
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hyperolius tuberilinguis Smith, 1849",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((25.5395 -28.5786,20.509 -28.5786,20.509 -32.9007,25.5395 -32.9007,25.5395 -28.5786))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034427-250525065834625/datasets/export for details.
DOI: 10.6084/m9.figshare.28229990.v1
title: Integrative phylogenomics positions sponges at the root of the animal tree

description: Additional data associated with a project that used integrative phylogenomics to root the animal tree.
DOI: 10.15468/dl.ajccc4
title: Occurrence Download

description: A dataset containing 3360721 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Plantae, Undaria pinnatifida (Harv.) Suringar)",
 "Country is New Zealand",
 "OccurrenceStatus is Present",
 "Year is greater than or equal to 0",
 "DecimalLongitude is greater than or equal to -180",
 "DecimalLatitude is greater than or equal to -90",
 {
 "not" : {
 "or" : [
 "Issue is Zero coordinate",
 "Issue is Coordinate out of range",
 "Issue is Country coordinate mismatch"
 ]
 }
 }
 ]
}

 The dataset includes 3360721 records from 282 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034404-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2zuq2u
title: Occurrence Download

description: A dataset containing 20 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Material sample)"
 ]
 }
 },
 "TaxonKey is Thallomys loringi (Heller, 1909)",
 "Continent is one of (Africa)",
 "Year is greater than or equal to 1900"
 ]
}

 The dataset includes 20 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034431-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.4121/adb0b003-4912-4bdb-b12f-e56d18c3b937.v2
title: Dataset underlying the publication: Response of a turbulent boundary layer to steady, square-wave-type transverse wall forcing

description: This dataset contains experimental data accompanying a publication investigating the efficacy of wall-based forcing of a zero pressure gradient turbulent boundary layer. Streamwise periodic forcing is applied experimentally by a series of 48 spanwise running belts, where we investigate the variation of wavelength between its near-optimum (as per the literature), sub-optimum, and post-optimum value. Multi-camera 2D-2C particle image velocimetry measurements were employed in the streamwise-wall-normal plane (x-y), capturing two fields-of-view (FOV), at the initiation of forcing (FOV1) and downstream (FOV2) where the control is fully established.All data underlying the figures, accompanied by the wind-tunnel conditions and relevant scaling parameters, such that they can be reproduced, will be published in .mat format for the individual figures. In addition, we publish the full dataset of the mean of the streamwise velocity component (U), and the higher-order streamwise (uu), streamwise-wall-normal (uv), and wall-normal (vv) Reynolds shear stresses, included in the data of fig7-8 and fig10The dataset is published for reproducibility, comparison, and to allow for future studies by other investigators.Under the CC BY 4.0 license, any reuse of this dataset should cite the original accompanying publication.
DOI: 10.15468/dl.hkb93h
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hyperolius tuberilinguis Smith, 1849",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((20.7576 -24.3688,20.529 -24.0715,20.509 -24.0715,20.509 -28.5786,25.5395 -28.5786,25.5395 -24.0715,25.1306 -24.0715,24.9333 -24.4571,23.8962 -24.2467,22.8065 -24.295,22.3719 -24.7207,22.2323 -25.547,22.0387 -25.7188,21.7833 -25.7784,21.5388 -25.7113,21.3553 -25.5415,20.7576 -24.3688))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034432-250525065834625/datasets/export for details.
DOI: 10.15468/dl.u845kd
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hyperolius tuberilinguis Smith, 1849",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((20.529 -24.0715,20.509 -24.0455,20.509 -24.0715,20.529 -24.0715))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034433-250525065834625/datasets/export for details.
DOI: 10.15468/dl.2x5ut7
title: Occurrence Download

description: A dataset containing 235 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Material sample)"
 ]
 }
 },
 "TaxonKey is Thallomys nigricauda (Thomas, 1882)",
 "Continent is one of (Africa)",
 "Year is greater than or equal to 1900"
 ]
}

 The dataset includes 235 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034434-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.azrvuz
title: Occurrence Download

description: A dataset containing 2511 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Geometry POLYGON((-71.18871 -34.46195,-71.18446 -34.46063,-71.18034 -34.45901,-71.17667 -34.45505,-71.17729 -34.45211,-71.18093 -34.44844,-71.1849 -34.44727,-71.18886 -34.45006,-71.19062 -34.44697,-71.20413 -34.44345,-71.20736 -34.43846,-71.21367 -34.4342,-71.25045 -34.42177,-71.26979 -34.44392,-71.27688 -34.45204,-71.27754 -34.45643,-71.25976 -34.46652,-71.25888 -34.47333,-71.30585 -34.45972,-71.31814 -34.45533,-71.31594 -34.46169,-71.28614 -34.47888,-71.31398 -34.50885,-71.31132 -34.51741,-71.29649 -34.52848,-71.30641 -34.55323,-71.32001 -34.55023,-71.32981 -34.5593,-71.34172 -34.57786,-71.34096 -34.59899,-71.33993 -34.60784,-71.32779 -34.6142,-71.31392 -34.59542,-71.28676 -34.59744,-71.28214 -34.60639,-71.26712 -34.61159,-71.23975 -34.6434,-71.152 -34.62335,-71.14256 -34.61181,-71.12965 -34.60797,-71.11739 -34.60106,-71.08697 -34.59529,-71.04856 -34.58944,-71.04203 -34.58844,-71.03584 -34.57746,-70.97598 -34.52469,-70.96814 -34.52049,-70.96851 -34.51492,-71.06425 -34.51247,-71.07743 -34.5159,-71.10074 -34.50788,-71.1158 -34.50595,-71.12212 -34.5081,-71.13354 -34.51198,-71.14171 -34.52236,-71.14806 -34.52692,-71.15124 -34.5292,-71.15447 -34.53427,-71.14447 -34.53778,-71.14583 -34.54292,-71.14276 -34.54743,-71.13691 -34.54836,-71.13443 -34.54889,-71.12875 -34.54942,-71.13123 -34.5567,-71.12899 -34.56764,-71.11877 -34.56696,-71.10712 -34.57126,-71.12061 -34.58104,-71.11972 -34.58255,-71.11517 -34.59026,-71.13199 -34.57885,-71.14718 -34.58242,-71.15229 -34.58971,-71.15032 -34.59626,-71.1536 -34.59448,-71.16334 -34.58375,-71.19869 -34.58666,-71.2182 -34.59238,-71.22583 -34.5813,-71.24119 -34.57755,-71.24425 -34.5768,-71.25927 -34.58376,-71.26551 -34.57981,-71.26644 -34.57429,-71.26564 -34.56838,-71.26328 -34.55929,-71.27307 -34.5616,-71.26298 -34.55627,-71.25911 -34.55217,-71.26053 -34.54921,-71.27134 -34.54935,-71.26486 -34.54546,-71.26039 -34.54719,-71.25259 -34.54526,-71.24385 -34.54023,-71.23676 -34.53869,-71.2341 -34.53811,-71.2194 -34.5267,-71.21762 -34.5085,-71.20141 -34.48439,-71.20772 -34.46442,-71.18871 -34.46195))",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 2511 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0034435-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29286497
title: Processed 10X Genomics scRNA-seq data for article: <i>Single-cell transcriptomics showed that maternal PCB exposure dysregulated ER stress-mediated cell type-specific responses in the liver of female offspring</i>

description: Polychlorinated biphenyls (PCBs) are persistent environmental toxicants that bioaccumulate in the food chain and readily cross the placenta, raising concerns for developmental toxicity.  While PCB exposure has been associated with metabolic and neurodevelopmental disorders, its cell type-specific effects on liver development remain poorly understood. This study aimed to investigate how maternal exposure to an environmentally relevant Fox River PCB mixture affects liver development in female offspring at single-cell resolution. We hypothesized that developmental PCB exposure disrupts hepatic metabolic and immune function in a cell-type-specific manner. From 2 weeks before mating, female dams were exposed to organic peanut oil mixed with organic peanut butter (vehicle, Trader Joe’s, Monrovia, CA) or the Fox River PCB mixture in vehicle (6 mg/kg body weight) once daily until pups were weaned at postnatal day (PND) 21.  Female mouse livers of the pups at PND 28 were collected.  Cryopreserved cells were thawed using a water bath at 37°C for 2 min, followed by serial dilution in DPBS until 32 ml was reached. Cells were centrifuged and resuspended in DPBS until a concentration of approximately 100 cells/μl was reached. The resuspended cells (n = 3), targeting 10 000 cells per sample, were then subject to scRNA-seq using a Chromium Next GEM single cell 3′ v3.1 kit and a Chromium X controller (10X Genomics, Pleasanton, CA) following the manufacturer’s instructions. The created libraries were then sequenced using the NovaSeq platform. Raw data were processed using the Cell Ranger v7.0 (10X Genomics, Pleasanton, CA).
DOI: 10.6084/m9.figshare.29286497.v1
title: Processed 10X Genomics scRNA-seq data for article: <i>Single-cell transcriptomics showed that maternal PCB exposure dysregulated ER stress-mediated cell type-specific responses in the liver of female offspring</i>

description: Polychlorinated biphenyls (PCBs) are persistent environmental toxicants that bioaccumulate in the food chain and readily cross the placenta, raising concerns for developmental toxicity.  While PCB exposure has been associated with metabolic and neurodevelopmental disorders, its cell type-specific effects on liver development remain poorly understood. This study aimed to investigate how maternal exposure to an environmentally relevant Fox River PCB mixture affects liver development in female offspring at single-cell resolution. We hypothesized that developmental PCB exposure disrupts hepatic metabolic and immune function in a cell-type-specific manner. From 2 weeks before mating, female dams were exposed to organic peanut oil mixed with organic peanut butter (vehicle, Trader Joe’s, Monrovia, CA) or the Fox River PCB mixture in vehicle (6 mg/kg body weight) once daily until pups were weaned at postnatal day (PND) 21.  Female mouse livers of the pups at PND 28 were collected.  Cryopreserved cells were thawed using a water bath at 37°C for 2 min, followed by serial dilution in DPBS until 32 ml was reached. Cells were centrifuged and resuspended in DPBS until a concentration of approximately 100 cells/μl was reached. The resuspended cells (n = 3), targeting 10 000 cells per sample, were then subject to scRNA-seq using a Chromium Next GEM single cell 3′ v3.1 kit and a Chromium X controller (10X Genomics, Pleasanton, CA) following the manufacturer’s instructions. The created libraries were then sequenced using the NovaSeq platform. Raw data were processed using the Cell Ranger v7.0 (10X Genomics, Pleasanton, CA).