cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7898543.v1
title: Spores of Plagiochila (Dumort.) Dumort.: the taxonomic relevance of morphology and ultrastructure

description: ABSTRACT Plagiochilaceae is a family of leafy liverworts that are distributed worldwide. It is of great importance due to its taxonomic and ecological implications among bryophytes. Most species of the family belong to the genus Plagiochila, but there is no consensus regarding its infrageneric circumscription. There have been few palynological studies involving Plagiochilaceae and Plagiochila. Here, we describe the spore morphology of seventeen species of Plagiochila and discuss the taxonomic value of palynological characters for these taxa. The spores were processed by standard palynological techniques and analyzed using light and electron microscopy. The spores were found to be apolar, spheroidal, released monads that vary in size from 13µm to 58µm (small to large size). The sporoderm comprises an intine (stratified), a nexine, and a sexine. The spore surface is ornamented with granules that vary in shape and morphology, thus allowing the studied species to be grouped into four spore types: regular and delicate granulate, irregular and coarse granulate, long granules with flattened apices, and long and straight granules. Hierarchical cluster analysis revealed five different groups of species, evidencing the importance of spore information for taxonomic and phylogenetic studies.
DOI: 10.6084/m9.figshare.7898525
title: First - line, non - cryopreserved autologous stem cell transplant for poor - risk germ - cell tumors: Experience in a developing country

description: ABSTRACT Purpose: The current first - line treatment for non - seminomatous germ cell tumor (NSGCT) consists of four cycles of cisplatin, etoposide, and bleomycin (BEP), which results in 5 - year overall survival &lt; 60% in patients with poor - risk features. Autologous hematopoietic stem cell transplantation (auto - HSCT) as a method for overcoming high toxicity after high dose chemotherapy (HDC) has been explored in different solid tumors, but has remained standard practice only for NSGCT. Our objective was to describe outcomes of patients with poor - risk NSGCT who underwent first - line autologous HSCT in a tertiary center in Mexico. Patients and Methods: Twenty nine consecutive patients with NSGCT who received first - line, non - cryopreserved autologous HSCT at the National Institute of Medical Sciences and Nutrition Salvador Zubiran in Mexico City, Mexico, from November 1998 to June 2016, were retrospectively analyzed. Results: The median age at transplantation was 23 (15 – 39) years. Most patients (n = 18, 62%) had testicular primary tumor, and 23 had metastases (79%). Complete response after auto - HSCT was observed in 45%. Non - relapse mortality was 0. Five - year relapse / progression free and overall survival were 67% and 69%, respectively. Conclusions: This small single limited - resource institution study demonstrated that patients with poor - risk NSGCT are curable by first - line HDC plus autologous HSCT and that this procedure is feasible and affordable to perform using non - cryopreserved hematopoietic stem cells.
DOI: 10.6084/m9.figshare.7898528
title: Relationship of Inter-Arm Systolic Blood Pressure Difference with Subclavian Artery Stenosis and Vertebral Artery Stenosis in Patients Undergoing Carotid Endarterectomy

description: Abstract Introduction: The aim of this study was to examine the association of inter-arm systolic blood pressure difference (IASBPD) with carotid artery stenosis, subclavian artery stenosis and vertebral artery stenosis in patients who underwent carotid endarterectomy. Methods: A total of 141 patients (29 females, 112 males; mean age 71.2±10.4 years; range 47 to 92 years) who underwent carotid endarterectomy between September 2010 and December 2017 were retrospectively evaluated. We classified patients into four groups according to the IASBPD ˂ 10 mmHg, ≥ 10 mm Hg, ≥ 20 mmHg and ≥ 30 mmHg. The stenosis of both subclavian and vertebral arteries was considered as ≥ 50%. Results: Of the 141 patients, 44 (31.2%) had ≥ 10 mmHg, 29 (20.5%) had ≥ 20 mmHg and 4 (2.8%) had ≥ 30 mmHg of IASBPD. 26 patients (18.4%) were diagnosed with significant subclavian artery stenosis and 18 (69.2%) of them had more than 20 mmHg of IASBPD. Of the 29 patients with IASBPD ≥ 20 mmHg, 19 patients (65.5%) had a significant subclavian artery stenosis. We found a significant correlation between preoperative symptoms and subclavian artery stenosis (P=0.018) and overall perioperative stroke was seen more frequently in patients with subclavian artery stenosis (P=0.041). A significant positive correlation was observed between vertebral artery stenosis and subclavian artery stenosis (P=0.01). Conclusion: Patients who were diagnosed with both subclavian artery stenosis and IASBPD (≥ 20 mmHg) had a higher risk of postoperative stroke and death, had higher total cholesterol, LDL-C, blood creatinine level, and were more symptomatic.
DOI: 10.6084/m9.figshare.7898537.v1
title: Use of Mini-FLOTAC and Fill-FLOTAC for rapidly diagnosing parasitic infections in zoo mammals

description: Abstract Animals reared in restricted environments are highly susceptible to gastrointestinal infection by helminths and protozoa and therefore zoos are characterized as being parasite-rich environments. Successful implementation of control programs of these parasites in zoo environment depends upon precise and rapid diagnosing of gastrointestinal infections. The aim of this study was to demonstrate the role of the Mini-FLOTAC technique in combination with Fill-FLOTAC for rapidly diagnosing parasitic infections in zoo mammals. Fecal samples were collected from 70 animals in four different zoos located in central and southern Italy. All the samples were analyzed using Mini-FLOTAC in combination with Fill-FLOTAC. Out of the 70 pooled samples examined, 80% (24/30) were positive for at least one parasite. Among the gastrointestinal nematodes, Strongyles were the most frequent (40%), followed by Trichuris spp. (23.3%), Parascaris spp. (13.3%) and Capillaria spp. (3.3%). Among the protozoa, Blastocystis spp., Giardia spp. and Eimeria spp. were detected in 6.6%, 3.3% and 3.3%, respectively. These results show that Mini-FLOTAC in combination with Fill-FLOTAC can be used, not only for rapidly diagnosing parasitic infections in zoo mammals, but also for monitoring control programs in which large numbers of fecal samples need to be examined rapidly and reliably.
DOI: 10.6084/m9.figshare.7898525.v1
title: First - line, non - cryopreserved autologous stem cell transplant for poor - risk germ - cell tumors: Experience in a developing country

description: ABSTRACT Purpose: The current first - line treatment for non - seminomatous germ cell tumor (NSGCT) consists of four cycles of cisplatin, etoposide, and bleomycin (BEP), which results in 5 - year overall survival &lt; 60% in patients with poor - risk features. Autologous hematopoietic stem cell transplantation (auto - HSCT) as a method for overcoming high toxicity after high dose chemotherapy (HDC) has been explored in different solid tumors, but has remained standard practice only for NSGCT. Our objective was to describe outcomes of patients with poor - risk NSGCT who underwent first - line autologous HSCT in a tertiary center in Mexico. Patients and Methods: Twenty nine consecutive patients with NSGCT who received first - line, non - cryopreserved autologous HSCT at the National Institute of Medical Sciences and Nutrition Salvador Zubiran in Mexico City, Mexico, from November 1998 to June 2016, were retrospectively analyzed. Results: The median age at transplantation was 23 (15 – 39) years. Most patients (n = 18, 62%) had testicular primary tumor, and 23 had metastases (79%). Complete response after auto - HSCT was observed in 45%. Non - relapse mortality was 0. Five - year relapse / progression free and overall survival were 67% and 69%, respectively. Conclusions: This small single limited - resource institution study demonstrated that patients with poor - risk NSGCT are curable by first - line HDC plus autologous HSCT and that this procedure is feasible and affordable to perform using non - cryopreserved hematopoietic stem cells.
DOI: 10.6084/m9.figshare.7898528.v1
title: Relationship of Inter-Arm Systolic Blood Pressure Difference with Subclavian Artery Stenosis and Vertebral Artery Stenosis in Patients Undergoing Carotid Endarterectomy

description: Abstract Introduction: The aim of this study was to examine the association of inter-arm systolic blood pressure difference (IASBPD) with carotid artery stenosis, subclavian artery stenosis and vertebral artery stenosis in patients who underwent carotid endarterectomy. Methods: A total of 141 patients (29 females, 112 males; mean age 71.2±10.4 years; range 47 to 92 years) who underwent carotid endarterectomy between September 2010 and December 2017 were retrospectively evaluated. We classified patients into four groups according to the IASBPD ˂ 10 mmHg, ≥ 10 mm Hg, ≥ 20 mmHg and ≥ 30 mmHg. The stenosis of both subclavian and vertebral arteries was considered as ≥ 50%. Results: Of the 141 patients, 44 (31.2%) had ≥ 10 mmHg, 29 (20.5%) had ≥ 20 mmHg and 4 (2.8%) had ≥ 30 mmHg of IASBPD. 26 patients (18.4%) were diagnosed with significant subclavian artery stenosis and 18 (69.2%) of them had more than 20 mmHg of IASBPD. Of the 29 patients with IASBPD ≥ 20 mmHg, 19 patients (65.5%) had a significant subclavian artery stenosis. We found a significant correlation between preoperative symptoms and subclavian artery stenosis (P=0.018) and overall perioperative stroke was seen more frequently in patients with subclavian artery stenosis (P=0.041). A significant positive correlation was observed between vertebral artery stenosis and subclavian artery stenosis (P=0.01). Conclusion: Patients who were diagnosed with both subclavian artery stenosis and IASBPD (≥ 20 mmHg) had a higher risk of postoperative stroke and death, had higher total cholesterol, LDL-C, blood creatinine level, and were more symptomatic.
DOI: 10.6084/m9.figshare.7898534
title: LUMINOSITY LEVELS AFFECT THE INITIAL SEEDLINGS GROWTH AND NUTRIENT ACCUMULATION IN Khaya senegalensis A. JUSS

description: ABSTRACT Khaya senegalensis, is a promising species for forest production, as it has excellent timber properties and adapts well to local edaphoclimatic conditions. To achieve better results in plant production and growth, some factors are important, such as nutrients, water and light. Light interferes directly with photosynthesis, plant growth and biomass. This study aims to investigate the morphological response and nutrient accumulation in seedlings of Khaya senegalensis under luminosity levels. An experiment was installed in the forest nursery during 150 days. The seedlings were distributed in a completely randomized design with five treatments in luminosity levels (full sun, 70%, 50%, 30% and natural shade), and 16 replicates. Height and diameter were measured in 30 days intervals. At the end of the experiment, mass weights (leaf, stem, root and total) were measured, and the root-shoot ratio and seed quality index proposed by Dickson were calculated. The accumulated macro and micronutrient contents were determined from the dry leaf material. The highest plants, diameters and total dry matter were found at levels of 70%, 50%, 30%, respectively. Plants exposed to natural shade grew less, in relation to plants with higher luminosity levels. As for nutrient accumulation, plants under natural shade accumulated higher levels of iron, while plants in the full sun accumulated more nitrogen contents. Plants with partial light conditions (70%, 50%, and 30%) were heavier in total biomass. Thus, the initial growth of Khaya senegalensis in low light conditions is compromised, whereas partial luminosities favor the growth and quality of seedlings.
DOI: 10.6084/m9.figshare.7898519
title: Treatment of a subdural empyema complicated by intracerebral abscess due to Brucella infection

description: A 55-year-old male presented with fever, stupor, aphasia, and left hemiparesis. A history of head trauma 3 months before was also reported. Cranial magnetic resonance imaging revealed slight contrast enhancement of lesions under the right frontal skull plate and right frontal lobe. Because of deterioration in nutritional status and intracranial hypertension, the patient was prepared for burr hole surgery. A subdural empyema (SDE) recurred after simple drainage. After detection of Brucella species in SDE, craniotomy combined with antibiotic treatment was undertaken. The patient received antibiotic therapy for 6 months (two doses of 2 g ceftriaxone, two doses of 100 mg doxycycline, and 700 mg rifapentine for 6 months) that resulted in complete cure of the infection. Thus, it was speculated that the preexisting subdural hematoma was formed after head trauma, which was followed by a hematogenous infection caused by Brucella species.
DOI: 10.6084/m9.figshare.7898522
title: EFFECT OF RESIDUAL EFFECTIVE ALKALI ON EUCALYPTUS KRAFT PULP YIELD AND CHEMISTRY

description: ABSTRACT The impact of residual effective alkali (REA) and pulp delignification degree on Eucalyptus kraft pulp yield and chemistry was investigated. In this study four types of eucalypt clones were evaluated. The eucalypt woods were cooked to kappa numbers of 14±1, 18±1, 22±1 and 26±1 using two different ranges of REA, namely: low-REA (1-2 g.L-1) and high-REA (8-9 g.L-1). In general, the low-REA cooks lead to pulps of higher yield, viscosity and xylans content and lower brightness and hexenuronic acid (HexA) content, in relation to the high-REA cooks. In addition, the low-REA cooks required about 20% less effective alkali to achieve the target kappa, in spite of demanding about 50% more H-factor. Similar trends were observed for the other woods and kappa values evaluated, but the level of gains varied substantially among woods and delignification degrees. Among the various woods, E. globulus was the easiest to delignify. There was a trend of higher brown pulp yield for the cooks terminated at higher kappa in the range of 14-26, but the lignin-HexA free screen yields were about similar in the kappa range of 18-26 for a given wood type.
DOI: 10.6084/m9.figshare.7898534.v1
title: LUMINOSITY LEVELS AFFECT THE INITIAL SEEDLINGS GROWTH AND NUTRIENT ACCUMULATION IN Khaya senegalensis A. JUSS

description: ABSTRACT Khaya senegalensis, is a promising species for forest production, as it has excellent timber properties and adapts well to local edaphoclimatic conditions. To achieve better results in plant production and growth, some factors are important, such as nutrients, water and light. Light interferes directly with photosynthesis, plant growth and biomass. This study aims to investigate the morphological response and nutrient accumulation in seedlings of Khaya senegalensis under luminosity levels. An experiment was installed in the forest nursery during 150 days. The seedlings were distributed in a completely randomized design with five treatments in luminosity levels (full sun, 70%, 50%, 30% and natural shade), and 16 replicates. Height and diameter were measured in 30 days intervals. At the end of the experiment, mass weights (leaf, stem, root and total) were measured, and the root-shoot ratio and seed quality index proposed by Dickson were calculated. The accumulated macro and micronutrient contents were determined from the dry leaf material. The highest plants, diameters and total dry matter were found at levels of 70%, 50%, 30%, respectively. Plants exposed to natural shade grew less, in relation to plants with higher luminosity levels. As for nutrient accumulation, plants under natural shade accumulated higher levels of iron, while plants in the full sun accumulated more nitrogen contents. Plants with partial light conditions (70%, 50%, and 30%) were heavier in total biomass. Thus, the initial growth of Khaya senegalensis in low light conditions is compromised, whereas partial luminosities favor the growth and quality of seedlings.
DOI: 10.6084/m9.figshare.7898510
title: Does the incremental shuttle walk test require maximal effort in young obese women?

description: Obesity is a chronic disease with a multifaceted treatment approach that includes nutritional counseling, structured exercise training, and increased daily physical activity. Increased body mass elicits higher cardiovascular, ventilatory and metabolic demands to varying degrees during exercise. With functional capacity assessment, this variability can be evaluated so individualized guidance for exercise training and daily physical activity can be provided. The aim of the present study was to compare cardiovascular, ventilatory and metabolic responses obtained during a symptom-limited cardiopulmonary exercise test (CPX) on a treadmill to responses obtained by the incremental shuttle walk test (ISWT) in obese women and to propose a peak oxygen consumption (VO2) prediction equation through variables obtained during the ISWT. Forty obese women (BMI ≥30 kg/m2) performed one treadmill CPX and two ISWTs. Heart rate (HR), arterial blood pressure (ABP) and perceived exertion by the Borg scale were measured at rest, during each stage of the exercise protocol, and throughout the recovery period. The predicted maximal heart rate (HRmax) was calculated (210 – age in years) (16) and compared to the HR response during the CPX. Peak VO2 obtained during CPX correlated significantly (P&lt;0.05) with ISWT peak VO2 (r=0.79) as well as ISWT distance (r=0.65). The predictive model for CPX peak VO2, using age and ISWT distance explained 67% of the variability. The current study indicates the ISWT may be used to predict aerobic capacity in obese women when CPX is not a viable option.
DOI: 10.6084/m9.figshare.7898513
title: Use of the Wasserman equation in optimization of the duration of the power ramp in a cardiopulmonary exercise test: a study of Brazilian men

description: This study aimed to analyze the agreement between measurements of unloaded oxygen uptake and peak oxygen uptake based on equations proposed by Wasserman and on real measurements directly obtained with the ergospirometry system. We performed an incremental cardiopulmonary exercise test (CPET), which was applied to two groups of sedentary male subjects: one apparently healthy group (HG, n=12) and the other had stable coronary artery disease (n=16). The mean age in the HG was 47±4 years and that in the coronary artery disease group (CG) was 57±8 years. Both groups performed CPET on a cycle ergometer with a ramp-type protocol at an intensity that was calculated according to the Wasserman equation. In the HG, there was no significant difference between measurements predicted by the formula and real measurements obtained in CPET in the unloaded condition. However, at peak effort, a significant difference was observed between oxygen uptake ( V ˙O2)peak(predicted) and V ˙O2peak(real) (nonparametric Wilcoxon test). In the CG, there was a significant difference of 116.26 mL/min between the predicted values by the formula and the real values obtained in the unloaded condition. A significant difference in peak effort was found, where V ˙O2peak(real) was 40% lower than V ˙O2peak(predicted) (nonparametric Wilcoxon test). There was no agreement between the real and predicted measurements as analyzed by Lin’s coefficient or the Bland and Altman model. The Wasserman formula does not appear to be appropriate for prediction of functional capacity of volunteers. Therefore, this formula cannot precisely predict the increase in power in incremental CPET on a cycle ergometer.
DOI: 10.6084/m9.figshare.7898519.v1
title: Treatment of a subdural empyema complicated by intracerebral abscess due to Brucella infection

description: A 55-year-old male presented with fever, stupor, aphasia, and left hemiparesis. A history of head trauma 3 months before was also reported. Cranial magnetic resonance imaging revealed slight contrast enhancement of lesions under the right frontal skull plate and right frontal lobe. Because of deterioration in nutritional status and intracranial hypertension, the patient was prepared for burr hole surgery. A subdural empyema (SDE) recurred after simple drainage. After detection of Brucella species in SDE, craniotomy combined with antibiotic treatment was undertaken. The patient received antibiotic therapy for 6 months (two doses of 2 g ceftriaxone, two doses of 100 mg doxycycline, and 700 mg rifapentine for 6 months) that resulted in complete cure of the infection. Thus, it was speculated that the preexisting subdural hematoma was formed after head trauma, which was followed by a hematogenous infection caused by Brucella species.
DOI: 10.6084/m9.figshare.7898522.v1
title: EFFECT OF RESIDUAL EFFECTIVE ALKALI ON EUCALYPTUS KRAFT PULP YIELD AND CHEMISTRY

description: ABSTRACT The impact of residual effective alkali (REA) and pulp delignification degree on Eucalyptus kraft pulp yield and chemistry was investigated. In this study four types of eucalypt clones were evaluated. The eucalypt woods were cooked to kappa numbers of 14±1, 18±1, 22±1 and 26±1 using two different ranges of REA, namely: low-REA (1-2 g.L-1) and high-REA (8-9 g.L-1). In general, the low-REA cooks lead to pulps of higher yield, viscosity and xylans content and lower brightness and hexenuronic acid (HexA) content, in relation to the high-REA cooks. In addition, the low-REA cooks required about 20% less effective alkali to achieve the target kappa, in spite of demanding about 50% more H-factor. Similar trends were observed for the other woods and kappa values evaluated, but the level of gains varied substantially among woods and delignification degrees. Among the various woods, E. globulus was the easiest to delignify. There was a trend of higher brown pulp yield for the cooks terminated at higher kappa in the range of 14-26, but the lignin-HexA free screen yields were about similar in the kappa range of 18-26 for a given wood type.
DOI: 10.6084/m9.figshare.7898507
title: 6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway

description: This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM BIO exhibited detectable β-catenin expression in their nuclei, and showed upregulated β-catenin and glycogen synthase kinase 3 beta(GSK3β) phosphorylation compared to untreated cells. In addition, BIO enhanced the mRNA expression of osteoblast differentiation markers such as ALP, runt-related transcription factor 2, collagen I, osteocalcin, and osteonectin. In conclusion, BIO upregulated GSK3β phosphorylation and inhibited its activity, thereby activating the Wnt/β-catenin signaling pathway and promoting the osteogenic differentiation of canine BMSCs. The effect of 1.0 μM BIO on BMSCs differentiation was stronger than that of 0.5 μM BIO.