cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12027207
title: Isolation, in vitro and in silico Evaluation of Phenylethanoid Glycoside from Arrabidaea brachypoda as Lipoxygenase Inhibitor

description: Lipoxygenase (LOX) plays an important role in inflammatory processes and Arrabidaea brachypoda (DC.) Bureau (Bignoniaceae) has been described as presenting anti-inflammatory activity. Therefore, the objective of this study was to develop a high-performance liquid chromatography (HPLC) procedure to directly recognize LOX inhibitor compounds in A. brachypoda crude extract, facilitating the isolation, characterization of bioactive compounds, evaluation of natural compounds using an in vitro 15-LOX assay and prediction of the most probable binding modes of their main constituent through molecular docking simulations. The chemical analysis was performed by ethanol crude extract microfractionation using HPLC-DAD (diode array detector) associated to a fraction collector. The bioactive chromatogram displayed a peak with 50.9% LOX inhibition at 13.6 min retention time. The extract was purified and conandroside was isolated, presenting a LOX inhibitory activity with an inhibitory concentration (IC50) of 7.8 ± 1.1 µM, close to standard quercetin (IC50 7.6 ± 0.3 µM). Additionally, conandroside was not cytotoxic to normal cells (GM07492A). The LOX-conandroside complex displayed a slightly higher docking score (92.7) than quercetin (71.5). These results together suggest that conandroside could be explored as lipoxygenase inhibitor.
DOI: 10.6084/m9.figshare.12027213.v1
title: Thoracic Aortic Aneurysm Surgery in Marfan Patients: a Perspective from the UK

description: Abstract Objective: Cardiovascular complications in Marfan patients include progressive aortic root dilation which can precipitate acute aortic dissection, ruptured aorta, severe aortic regurgitation, or all the aforementioned. Such complications can be fatal and the cause of death prior to any surgical intervention. We set out to identify the Marfan population in England and Wales and present their surgical outcomes. Methods: A total of 306 patients with Marfan syndrome who underwent aortic root surgery were identified between April 2007 and March 2013 from NICOR database. We examined the perioperative characteristics of such cohort along with in-hospital outcomes and survival. Results: Root and ascending segment procedures on Marfan patients performed in 3.3% of the total cohort by NICOR root surgery patients. The median reported age was 40 years (IQR = 29-49 years) and 100 (32.7%) were female. Of the patients analysed, 17.3% were treated non-electively and 68.6% of them received concomitant valve procedure. The in-hospital mortality was 2.0%. Reoperation for bleeding was required in 8.2% of patients and 1.3% of them suffered a cerebrovascular accident (CVA). Mortality at 1 year was reported as 5.5%. Conclusion: The outcomes of surgery on the root and ascending aorta in Marfan patients in the United Kingdom are satisfactory; however, the overall complexities of this patient population are not well understood and would benefit from further investigations.
DOI: 10.6084/m9.figshare.12027201
title: Comparative Evaluation of the Effects of Binzalkonium Chloride, Iodine, Gluteraldehyde and Hydrogen Peroxide Disinfectants against Avian Salmonellae Focusing on Genotypic Resistance Pattern of the Salmonellae Serotypes toward Benzalkonium Chloride

description: ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations (1:100, 200 and 400) at different temperatures and periods. Bacterial count was performed before and after the treatment. PCR for presence of qacEΔ1 gene was also performed before and after the treatment. The biocidal effect of the disinfectants found to be dependent on concentration, temperature and treatment period in addition to the type of the disinfectant. Hydrogen peroxide proved to be the most active agent followed by gluteradehyde, iodine and benzalkonium chloride. A link between the resistance against benzalkonium chloride and the existence of qacEΔ1 gene was proven in S. Typhimurium, whether treated or not treated with benzalkonium chloride.
DOI: 10.6084/m9.figshare.12027207.v1
title: Isolation, in vitro and in silico Evaluation of Phenylethanoid Glycoside from Arrabidaea brachypoda as Lipoxygenase Inhibitor

description: Lipoxygenase (LOX) plays an important role in inflammatory processes and Arrabidaea brachypoda (DC.) Bureau (Bignoniaceae) has been described as presenting anti-inflammatory activity. Therefore, the objective of this study was to develop a high-performance liquid chromatography (HPLC) procedure to directly recognize LOX inhibitor compounds in A. brachypoda crude extract, facilitating the isolation, characterization of bioactive compounds, evaluation of natural compounds using an in vitro 15-LOX assay and prediction of the most probable binding modes of their main constituent through molecular docking simulations. The chemical analysis was performed by ethanol crude extract microfractionation using HPLC-DAD (diode array detector) associated to a fraction collector. The bioactive chromatogram displayed a peak with 50.9% LOX inhibition at 13.6 min retention time. The extract was purified and conandroside was isolated, presenting a LOX inhibitory activity with an inhibitory concentration (IC50) of 7.8 ± 1.1 µM, close to standard quercetin (IC50 7.6 ± 0.3 µM). Additionally, conandroside was not cytotoxic to normal cells (GM07492A). The LOX-conandroside complex displayed a slightly higher docking score (92.7) than quercetin (71.5). These results together suggest that conandroside could be explored as lipoxygenase inhibitor.
DOI: 10.6084/m9.figshare.12027201.v1
title: Comparative Evaluation of the Effects of Binzalkonium Chloride, Iodine, Gluteraldehyde and Hydrogen Peroxide Disinfectants against Avian Salmonellae Focusing on Genotypic Resistance Pattern of the Salmonellae Serotypes toward Benzalkonium Chloride

description: ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations (1:100, 200 and 400) at different temperatures and periods. Bacterial count was performed before and after the treatment. PCR for presence of qacEΔ1 gene was also performed before and after the treatment. The biocidal effect of the disinfectants found to be dependent on concentration, temperature and treatment period in addition to the type of the disinfectant. Hydrogen peroxide proved to be the most active agent followed by gluteradehyde, iodine and benzalkonium chloride. A link between the resistance against benzalkonium chloride and the existence of qacEΔ1 gene was proven in S. Typhimurium, whether treated or not treated with benzalkonium chloride.
DOI: 10.6084/m9.figshare.12027177
title: Metabolization of Insecticidal Amides from Leaves of Piper tuberculatum by Heraclydes hectorides and Naupactus bipes

description: Amides have been recognized as potent insecticidal natural products but, despite their variety of targets and mechanisms of action, their metabolic fate in insects is virtually unknown. The currently accepted hypothesis is that specialist herbivores are capable of biotransforming xenobiotics rendering them more polar and excretable while generalist insects do not have comparable capacity. The leaves from Piper tuberculatum, rich in insecticide amides, were offered to two insect species found on Piper leaves under natural conditions and also to four generalist grasshoppers in order to compare their capacity of biotransforming xenobiotics. The amides 1-7 were identified in the P. tuberculatum leaves and their corresponding carboxylic acids 8-13 were detected in frass samples of two host insects suggesting that these species promote the amides hydrolysis. The four generalist grasshoppers when offered P. tuberculatum leaves, starved to death after 72 h, indicating a strong antifeedant activity of P. tuberculatum leaves.
DOI: 10.6084/m9.figshare.12027171
title: Sodium Requirement for Muscovy Ducks in Housing

description: ABSTRACT The present study aimed to determine the ideal sodium levels for muscovy ducks in housing. Two hundred and forty muscovy ducks of creole lineage were used, distributed in boxes with water and food ad libitum. The experimental design was completely randomized, where treatments were constituted by six nutritional plans (initial, growth and termination) with different sodium levels, and four replicates of 10 muscovy ducks each. The birds had weekly performance evaluations, and after 90 days, eight birds (four males and four females) in each treatment were slaughtered for evaluation of carcass traits. Data collected were subjected to Tukey test at 5% of significance. Differences were observed (p&lt;0.05) in performance (feed intake and feed conversion), where average levels of sodium presented better results. In carcass yields, average levels presented a positive influence (p&lt;0.05) on muscovy duck growth. Male muscovy ducks presented better feed efficiency than females in the same period. The present study indicates that nutritional plan 3 (initial = 0.25%; growth = 0.30% and termination = 0.35%) showed better nutritional requirements of sodium for muscovy ducks in housing, obtaining better performance and carcass development.
DOI: 10.6084/m9.figshare.12027174
title: Impact of catastrophizing on pain during orthodontic treatment

description: ABSTRACT Objective: This study proposed to investigate the influence of catastrophizing and others factors related to pain during orthodontic treatment. Methods: 27 patients with 0.022 x 0.028-in Straight-wire brackets were evaluated during alignment and leveling phase with nickel-titanium wires. Visual Analog Scales measured the intensity of orthodontic pain at six moments after a clinical appointment: 6 first hours; 1, 2, 3, 5, and 7 days. Multiple linear regression and stepwise approach assessed the influence of the following variables on pain: catastrophizing, sex, age, duration of treatment, clinical appointment time (morning or afternoon), and wire diameter. Results: The highest pain intensity was reported 24 hours after activation. These data were used to analyze factors associated with pain level. Age (r = 0.062, p= 0.7586), sex (p= 0.28), catastrophizing (r = -0.268, p= 0.1765), and orthodontic wire diameter (r = 0.0245, p= 0.2181) were not correlated with orthodontic pain in the univariate statistics. Catastrophizing was included in the multiple regression model because it was of great interest. Duration of orthodontic treatment (r = 0.6045, p= 0.0008) and the time when orthodontic appliance was activated (p= 0.0106) showed statistical significant associations with pain, and were also included in the multivariate regression, which showed that about 32% of orthodontic pain could be explained by the duration of treatment (R2= 0.32, p= 0.0475). Catastrophizing (R2= 0.0006, p= 0.8881) and clinical appointment time were not significantly associated with pain (R2= 0.037, p= 0.2710). Conclusions: Pain after activation of fixed orthodontic appliance is not associated with catastrophizing as well as age, sex, orthodontic wire diameter, and period of activation.
DOI: 10.6084/m9.figshare.12027177.v1
title: Metabolization of Insecticidal Amides from Leaves of Piper tuberculatum by Heraclydes hectorides and Naupactus bipes

description: Amides have been recognized as potent insecticidal natural products but, despite their variety of targets and mechanisms of action, their metabolic fate in insects is virtually unknown. The currently accepted hypothesis is that specialist herbivores are capable of biotransforming xenobiotics rendering them more polar and excretable while generalist insects do not have comparable capacity. The leaves from Piper tuberculatum, rich in insecticide amides, were offered to two insect species found on Piper leaves under natural conditions and also to four generalist grasshoppers in order to compare their capacity of biotransforming xenobiotics. The amides 1-7 were identified in the P. tuberculatum leaves and their corresponding carboxylic acids 8-13 were detected in frass samples of two host insects suggesting that these species promote the amides hydrolysis. The four generalist grasshoppers when offered P. tuberculatum leaves, starved to death after 72 h, indicating a strong antifeedant activity of P. tuberculatum leaves.
DOI: 10.6084/m9.figshare.12027168
title: Variation in root morphology of epiphytic orchids along small-scale and large-scale moisture gradients

description: ABSTRACT Root morphology is expected to respond to environmental conditions. Two earlier studies reported predictable changes in the structure of the velamen radicum (common in monocotyledons) along rainfall gradients, which was expected from its assumed role in plant-water-relations. The present study expanded on this idea by analysing nine root traits that can be linked to root function, including velamen structure, along two moisture gradients of different spatial scales: 1) along the vertical gradient of a lowland forest in central Panama and 2) along a strong regional rainfall gradient in western Panama. All studied traits (e.g. velamen width, number of xylem poles, lignification of the exodermis) of the 45 orchid species showed substantial intraspecific and interspecific variation, but none of this (within forest) or very little of this (regional gradient) was related to the gradients. Only the community weighted means of velamen width, stele width and xylem pole number varied significantly with rainfall, with substantial effect sizes only found in the latter two traits. Our results clearly disagree with those of earlier publications, which did not include species abundance and/or rigorous statistics in their analyses. This discrepancy highlights that our understanding of the link between velamen form and function is still rudimentary.
DOI: 10.6084/m9.figshare.12027171.v1
title: Sodium Requirement for Muscovy Ducks in Housing

description: ABSTRACT The present study aimed to determine the ideal sodium levels for muscovy ducks in housing. Two hundred and forty muscovy ducks of creole lineage were used, distributed in boxes with water and food ad libitum. The experimental design was completely randomized, where treatments were constituted by six nutritional plans (initial, growth and termination) with different sodium levels, and four replicates of 10 muscovy ducks each. The birds had weekly performance evaluations, and after 90 days, eight birds (four males and four females) in each treatment were slaughtered for evaluation of carcass traits. Data collected were subjected to Tukey test at 5% of significance. Differences were observed (p&lt;0.05) in performance (feed intake and feed conversion), where average levels of sodium presented better results. In carcass yields, average levels presented a positive influence (p&lt;0.05) on muscovy duck growth. Male muscovy ducks presented better feed efficiency than females in the same period. The present study indicates that nutritional plan 3 (initial = 0.25%; growth = 0.30% and termination = 0.35%) showed better nutritional requirements of sodium for muscovy ducks in housing, obtaining better performance and carcass development.
DOI: 10.6084/m9.figshare.12027174.v1
title: Impact of catastrophizing on pain during orthodontic treatment

description: ABSTRACT Objective: This study proposed to investigate the influence of catastrophizing and others factors related to pain during orthodontic treatment. Methods: 27 patients with 0.022 x 0.028-in Straight-wire brackets were evaluated during alignment and leveling phase with nickel-titanium wires. Visual Analog Scales measured the intensity of orthodontic pain at six moments after a clinical appointment: 6 first hours; 1, 2, 3, 5, and 7 days. Multiple linear regression and stepwise approach assessed the influence of the following variables on pain: catastrophizing, sex, age, duration of treatment, clinical appointment time (morning or afternoon), and wire diameter. Results: The highest pain intensity was reported 24 hours after activation. These data were used to analyze factors associated with pain level. Age (r = 0.062, p= 0.7586), sex (p= 0.28), catastrophizing (r = -0.268, p= 0.1765), and orthodontic wire diameter (r = 0.0245, p= 0.2181) were not correlated with orthodontic pain in the univariate statistics. Catastrophizing was included in the multiple regression model because it was of great interest. Duration of orthodontic treatment (r = 0.6045, p= 0.0008) and the time when orthodontic appliance was activated (p= 0.0106) showed statistical significant associations with pain, and were also included in the multivariate regression, which showed that about 32% of orthodontic pain could be explained by the duration of treatment (R2= 0.32, p= 0.0475). Catastrophizing (R2= 0.0006, p= 0.8881) and clinical appointment time were not significantly associated with pain (R2= 0.037, p= 0.2710). Conclusions: Pain after activation of fixed orthodontic appliance is not associated with catastrophizing as well as age, sex, orthodontic wire diameter, and period of activation.
DOI: 10.6084/m9.figshare.12027168.v1
title: Variation in root morphology of epiphytic orchids along small-scale and large-scale moisture gradients

description: ABSTRACT Root morphology is expected to respond to environmental conditions. Two earlier studies reported predictable changes in the structure of the velamen radicum (common in monocotyledons) along rainfall gradients, which was expected from its assumed role in plant-water-relations. The present study expanded on this idea by analysing nine root traits that can be linked to root function, including velamen structure, along two moisture gradients of different spatial scales: 1) along the vertical gradient of a lowland forest in central Panama and 2) along a strong regional rainfall gradient in western Panama. All studied traits (e.g. velamen width, number of xylem poles, lignification of the exodermis) of the 45 orchid species showed substantial intraspecific and interspecific variation, but none of this (within forest) or very little of this (regional gradient) was related to the gradients. Only the community weighted means of velamen width, stele width and xylem pole number varied significantly with rainfall, with substantial effect sizes only found in the latter two traits. Our results clearly disagree with those of earlier publications, which did not include species abundance and/or rigorous statistics in their analyses. This discrepancy highlights that our understanding of the link between velamen form and function is still rudimentary.
DOI: 10.6084/m9.figshare.12027144
title: Experimental Investigation on Mechanical Behaviors of Q345B Steel Material over Wide Ranges of Strain Rates and Temperatures

description: <b>1.the yield strength(fy), tensile strength (Rm) and elastic modulus (E) of Q345B steel at different temperatures</b> <b>2. the Johnson-cook constitutive equation of Q345B</b>
DOI: 10.6084/m9.figshare.12027144.v1
title: Experimental Investigation on Mechanical Behaviors of Q345B Steel Material over Wide Ranges of Strain Rates and Temperatures

description: <b>1.the yield strength(fy), tensile strength (Rm) and elastic modulus (E) of Q345B steel at different temperatures</b> <b>2. the Johnson-cook constitutive equation of Q345B</b>
DOI: 10.6084/m9.figshare.12027138
title: Supplementary table

description: Supplementary table
DOI: 10.6084/m9.figshare.12027129
title: VT20200324.Top16Covid19ImpactedCountries.png

description: Johns Hopkins Data
DOI: 10.6084/m9.figshare.12027138.v1
title: Supplementary table

description: Supplementary table
DOI: 10.6084/m9.figshare.12027063
title: LiJie

description: BACTERIA BGC CLUSTER
DOI: 10.6084/m9.figshare.12027129.v1
title: VT20200324.Top16Covid19ImpactedCountries.png

description: Johns Hopkins Data
DOI: 10.6084/m9.figshare.12027063.v1
title: LiJie

description: BACTERIA BGC CLUSTER
DOI: 10.6084/m9.figshare.12026997
title: SiHerbMiniArchive.xlsx

description: Synthesis of publications between years 1900 and 2020 in the Web of Science core collection database generated using search terms “silic*” and “herbivor*” and not “in silico”. We filtered the 413 results until only those publications that directly studied Si and herbivory remained (119). Synthesis terms include system, biome, plant functional group, plant, damaged part, herbivore type, herbivore guild/group.
DOI: 10.6084/m9.figshare.12026994
title: Alignments of Each Gene Used in Multi-gene Phylogenies

description: Alignments for each individual gene utilized for both multi-gene phylogenies. These genes were pulled from the core eukaryotic gene databases of CEGMA and BUSCO, as well as from the Janouškovec et al., 2017 study on Dinoflagellate phylogenetic relationships. <br>
DOI: 10.6084/m9.figshare.12026997.v1
title: SiHerbMiniArchive.xlsx

description: Synthesis of publications between years 1900 and 2020 in the Web of Science core collection database generated using search terms “silic*” and “herbivor*” and not “in silico”. We filtered the 413 results until only those publications that directly studied Si and herbivory remained (119). Synthesis terms include system, biome, plant functional group, plant, damaged part, herbivore type, herbivore guild/group.
DOI: 10.6084/m9.figshare.12026988
title: Janouškovec et al., 2017 Gene Alignment

description: Alignment generated with concatenated genes identified in Janouškovec et al., 2017 that were found to be single-copy.<br>
DOI: 10.6084/m9.figshare.12026991
title: Core Eukaryotic Genes Alignment

description: Alignment of concatenated genes pulled from the core eukaryotic gene databases of CEGMA and BUSCO that were found to be single copy in the species examined in this study.<br>
DOI: 10.6084/m9.figshare.12026979
title: rDNA D1-D3 Alignment

description: Alignments generated by isolating the D1-D3 region of each species in this study, and using previously submitted NCBI data.<br>
DOI: 10.6084/m9.figshare.12026988.v1
title: Janouškovec et al., 2017 Gene Alignment

description: Alignment generated with concatenated genes identified in Janouškovec et al., 2017 that were found to be single-copy.<br>