DOI: 10.5061/dryad.vb53h
title: Data from: Mate competition and evolutionary outcomes in genetically modified Zebrafish (Danio rerio)

description: Demonstrating relationships between sexual selection mechanisms and trait
 evolution is central to testing evolutionary theory. Using zebrafish, we
 found that wild-type males possessed a significant advantage in mate
 competition over transgenic RFP Glofish™ males. In mating trials,
 wild-type males were aggressively superior to transgenic males in
 male-male chases and male-female chases; as a result, wild-type males
 sired 2.5x as many young as did transgenic males. In contrast, an earlier
 study demonstrated that female zebrafish preferred transgenic males as
 mates when mate competition was excluded experimentally. We tested the
 evolutionary consequence of this conflict between sexual selection
 mechanisms in a long-term study. The predicted loss of the transgenic
 phenotype was confirmed. More than 18,500 adults collected from 18
 populations across 15 generations revealed that the frequency of the
 transgenic phenotype declined rapidly and was eliminated entirely in all
 but one population. Fitness component data for both sexes indicated that
 only male mating success differed between wild-type and transgenic
 individuals. Our predictive demographic model based on fitness components
 closely matched the rate of transgenic phenotype loss observed in the
 long-term study, thereby supporting its utility for studies assessing
 evolutionary outcomes of escaped or released genetically modified animals.
description: fry for sas
 analysisThese data provide the
 information on the number of fry of each genotype collected each day from
 each aquarium. Group is referred to as aquarium in the paper; number of
 fry refers to how many fry of each type were collected; pctredsires is the
 %red male category of which there are five types (25%, 33$, 50%, 66%, and
 75%; frytype is either red or wild-type; collection refers to the number
 of days that data were collected for each grouplong-term zebrafish
 studyThese data provide the
 information on tankno, generation, numred, numwt, pctredmorph, and
 simulationredpct. Tankno provides information on each of the 18 tanks;
 generation for each of the 18 tanks varies from 1 to a maximum of 15; how
 the generations were collected is explained in the paper as no overlapping
 generations were permitted, numred is the number of red adults counted at
 the end of each generation; numwt is the number of wild-type adults
 counted at the end of each generation; pctredmorph is calculated as:
 (numred/ numred + numwt) * 100; simulationredpct is the predicted percent
 red individuals by the demographic model described in the
 paper.malemale chases for
 sasThese data provide the
 information on the number of male directed chases toward another male and
 indicate if the male chasing was red or wild type, and if the male being
 chased was red or wild type. Data are given for group, %red male category,
 number of chases, collection, type of chase, and chases per male. Group is
 referred to as aquarium in the paper; pctred is the %red male category of
 which there are five types (25%, 33$, 50%, 66%, and 75%; number of chases
 observed is how many chases were observed during the 10 min observation
 period each day; collection refers to the number of days that data were
 collected for each group; Chasetype: 1 = wild-type male chasing a red
 male, 2 = a red male chasing a wild-type male; chasepermale divides the
 numbchases by how many males of each type were in the aquarium to allow
 for comparison of different %red male categories.malefemale chases for
 sasData file: Malefemale
 chases: These data provide the information on the number of male directed
 chases toward females and indicate if the male chasing was red or wild
 type. Data are given for collection, type of chase, number of chases,
 group, %red male category, chases per male, and chase type. Collection
 refers to the number of days that data were collected for each group, type
 of chase: either red male or wild-type male chasing a female, number of
 chases observed is how many chases were observed during the 10 min
 observation period each day; group is referred to as aquarium in the
 paper; pctred is the %red male category of which there are five types
 (25%, 33$, 50%, 66%, and 75%. Chasespermale divides the numbchases by how
 many males were in the aquarium to allow for comparison of different %red
 male categories. Chasetype codes typechase numerically (1 = wild-type male
 chasing female; 2 = red male chasing female).