DOI: 10.5061/dryad.51c59zwmm
title: Hormone type, soaking duration and seed coating affect dormancy and germination of pennycress (Thlaspi arvense) lines

description: Establishment of pennycress is key to ensure uniform stands and maximise
 canopy closure in autumn. Past work has demonstrated that selected seed
 treatments could improve germination and establishment, but the
 optimisation of these processes to minimise inputs and time have not been
 assessed. The objective of this work was to evaluate seed enhancement
 technologies (hormone application by soaking and seed coating) to overcome
 seed dormancy and/or enhance germination. Two black-seeded lines
 (‘MN106NS’ and ‘ARV1’) and one golden-seeded line (‘tt8-t/ARV1’) were
 examined. Seeds were soaked in gibberellic acid (GA4+7) or benzyladenine
 (6-BA), or distilled water for different durations (30, 60, 240 minutes)
 and compared to an unsoaked control. Results showed that the GA treatment
 significantly increased germination, particularly for ‘ARV1’ and ‘MN106NS’
 lines. Seed coating increased seed diameter but did not enhance
 germination; instead, it hindered ‘tt8-t/ARV1’ germination by 99-100%. The
 study suggests soaking seeds for at least 30 minutes in a GA solution to
 improve germination in black-seeded lines, however, economic and
 logistical factors must be considered. None of the tested enhancements
 proved beneficial on the two seed lots of golden-seeded line ‘tt8-t/ARV1’
 and further research is needed to examine more seed lots before
 conclusions can be made.
description: # Hormone type, soaking duration and seed coating affect dormancy and
 germination of pennycress (Thlaspi arvense) lines Dataset DOI:
 [10.5061/dryad.51c59zwmm](10.5061/dryad.51c59zwmm) ## Description of the
 data and file structure Data contained in this entry focuses solely on
 germination data after pennycress lines were treated with various
 processes. The Pellet_soak file contains black-seeded and golden-seeded
 pennycress lines and their germination after being soaked in water,
 gibberellic acid A4+A7, or benzyladenine for 30, 60, or 240 minutes. Total
 recorded germination on days 4, 7, and 10 are shared. The file called
 Pellet_process contains germination data of pennycress seeds after
 undergoing different intensities of processing that seeds will experience
 during seed pelleting. Seeds were stopped at different points in the
 pellet-building process to see if the increased amount of handling and
 agitation was contributing to changes in germination. Again, germination
 totals on days 4, 7, and 10 are recorded as well as information on 1000
 seed weight, average seed diameter, and change in weight of pelleted seeds
 after agitation. Missing data for a given variable within a plot is
 denoted solely with a “.” value as data was not or could not be collected
 in the specific instance. ### Files and variables #### File:
 Pellet_soak.csv **Description:** The dataset used to examine the effects
 of hormones and the soaking process on seed germination. ##### Variables *
 Block (Rep): Experimental run in time. * Treatment: Soaking treatment used
 for that experimental unit. * Pennycress Line: Pennycress line tested. The
 "-22" or "-21" following 'tt8' indicates the
 harvest year of the seed lot. * PlotID: Assigned experimental unit
 identifier. * 4 day total germination %: Cumulative percent germination on
 day 4. * 7 day total germination %: Cumulative percent germination on day
 7. * 10 day total germination %: Cumulative percent germination on day 10.
 #### File: Pellet_process.csv **Description:** The dataset used to analyze
 the pelleting process impact on seed germination of pennycress. #####
 Variables * Block (Rep): Experimental run in time. * Treatment: Applied
 component of the pelleting process used on this experimental unit. *
 Pennycress Line: Pennycress line tested. * PlotID: Assigned experimental
 unit identifier. * 4 day total germination %: Cumulative percent
 germination on day 4. * 7 day total germination %: Cumulative percent
 germination on day 7. * 10 day total germination %: Cumulative percent
 germination on day 10. * Weighted Average Seed Diameter (mm): Weighted
 average diameter of 100 seeds in mm. * Weight Difference (g): Change in
 weight of 5g of pelleted seed after agitation. ## Access information Other
 publicly accessible locations of the data: GEMS Informatics