DOI: 10.18150/tt8kis
title: Raw data obtained as the result of the antifungal effect of pyroligneous acid on the phytopathogenic fungus Botrytis cinerea

description: The deposited data was collected as part of the research project entitled: “Zmiany profilu fenotypowego i wrażliwości chemicznej patogenicznych dla upraw truskawki grzybów z gatunku Botrytis cinerea w wyniku działania octu drzewnego (łac. acetum pyro-lignosum)” (The phenotypic profile and chemical sensitivity of pathogenic for strawberry fungi beloging to the species of Botrytis cinerea under the influence of pyroligneous acid (lat. acetum pyro-lignosum)).This study was funded by the National Science Centre in the frame of the Miniatura-5 scientific research task, contract number DEC-2021/05/X/NZ9/01672 (isolates used in the experiments were first obtained as a part of the BIOSTRATEG3/344433/16/NCBR/2018 funded by The National Centre for Research and Development).In recent years, climate change has intensified harsh periods of rain alternating with periods of drought, leading to an increase in the presence of phytopathogenic fungi. In this study, we want to analyse the antifungal properties of pyroligneous acid against the fungal phytopathogen Botrytis cinerea. Through the inhibition test, we observed that the application of different dilutions of pyroligneous acid rarefied the growth of the fungal mycelium. Furthermore, we have seen through the metabolic profile that B. cinerea is not able to use pyroligneous acid as a resource or even grow in close contact with this resource. Moreover, we observed that the pre-incubation of the fungus in pyroligneous acid leads to a reduction in biomass production. These results give us hope for the possible use of this natural substance as a possible substance to protect plantations from pathogen attacks.The research was conducted from 15.12.2021 to 14.12.2022, at the Institute of Agrophysics, Polish Academy of Sciences, the Department of Soil and Plant System, Laboratory of Molecular and Environmental Microbiology.The data includes sets of biological data (metabolic profile analyses, sensitivity of fungi to different concentration of pyroligneous acid). Metabolic profile characterization was performed using BiologTM FF microplates, with readings performed at 490 nm and 750 nm wavelengths. Sensitivity data was obtained via inhibition zone measurements on Petri dishes, as well as absorbance measurements on BiologTM MT2 microplates, including the measurements of respiratory response of the tested isolates resulting from substrate catabolism (colorimetric reaction) (490 nm) and the level of cell biomass growth (measured by optical density) (750 nm).The dataset consists of the following filesFF_analysis - raw data regarding research results to evaluate fungal isolates’ metabolic profile in control conditions.FF_analysis_2 - raw data regarding research results to evaluate fungal isolates’ metabolic profile after pre-incubation under the influence of different concentrations of pyroligneous acid.Inhibition_test - results from disc-diffusion inhibition test on Petri dishes to evaluate the growth inhibitory properties of different concentrations of pyroligneous acid.MT2_analyses - raw data regarding research results to evaluate fungal isolates sensitivity to coincubation with different concentrations of pyroligneous acid.The data files were compiled based on conducted laboratory research. The results presented in the files were obtained using research equipment: microplate reader with a computer workstation - MicroStationTM (BiologTM Hayward, USA).