cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2006.2087
title: Annual variation and cellular source of renieramycins from the blue sponge Xestospongia sp.

description: The Thai blue sponge Xestospongia sp. was found to produce large amount of the highly potent cytotoxic bistetrahydroisoquinolines, named renieramycins. Pretreatment of the sponge with potassium cyanide before extraction yielded a mixture of stabilized renieramycins, of which renieramycin M was predominant. The purpose of this study was to determine the annual variation in quantity and to localize the cellular source of renieramycin M within the sponge. The sponge Xestospongia sp. samples were monthly collected from Sichang Island, Cholburi Province, in the Gulf of Thailand during August 2005 to July 2006. A high performance liquid chromatography (HPLC) method, which was developed to analyze renieramycins, consisted of a RP-18e column (125×4 mm, 5 μm), the isocratic solution of methanol/water (7:3) as the mobile phase at flow rate 1.0 mL/min, a photodiode array detector at wavelength 270 nm and acenaphthene as the internal standard. The HPLC method was reliable in terms of linearity (R² = 0.9999), accuracy (%recovery of 103.10-107.14%), and precision (%RSD of 0.39-0.95%). The HPLC analyses showed that renieramycin M was normally detected as the major component at retention time 8.9 min and the annual variation of renieramycin M quantity (%w/w, semi-dried weight sponge) was significantly different (ANOVA, p &lt; 0.05) with the average of 0.13%. However, the sponge produced the highest amount of renieramycin M at 0.20% w/w in April and October and the lowest at 0.08% w/w in January and at 0.09% w/w in July. Following the Pearson correlation coefficient (r), the relationships between renieramycin M annual content and other environment factors were found to have the positive correlations with total dissolved solids and chlorophyll-a. Fractionation of the sponge cells was performed by differential centrifugation and Ficoll density gradient centrifugation. The result showed that choanocytes and archaeocytes were the major cell types of the sponge. The HPLC analyses of cell fractions demonstrated that renieramycin M was associated with both sponge cells but not with the bacterial fraction
DOI: 10.14457/cu.the.2006.2088
title: Formulation development and Evaluation of Hydrophobic Base Containing Herbal extracts for Oral Ulcers

description: This research study tended to develop hydrophobic base with suitable gelling agents containing mangostin and/or asiaticoside for the relief of oral lichen planus and apthous ulcer. Hydrophobic base with good physical appearance was prepared from melting process of plyethylene polymer (PE) and mineral oil at about 80 degree Celsius. PE polymer in mineral oil was found to precipitate as small crystallites surrounded by long fibrous amorphous filaments which intermesh and produce a sponge-like structure resulting in a three dimentional lattice responsible for stable gel structure. Among various percentages of PE in this study, it was found that the appropriate amount of PE polymer in hydroiphobic base was 4.5 percent. From rheogram at various temperatures, hydrophobic base exhibits psuedoplastic behavior. Activation energy calculated from modified Arrhenius’s equation was about 12.45 kj/mol. Gelatin, xanthan gum, pectin, sodium evaluated for suitable gelling agent in hydrophobic base. Chitosan salts prepared by spray-drying process with suitable conditions were fine yellowish powder with round shape. Among these gelling agents mixed with hydrophobic base, SCMC, pectin, chitosan glutamate molecular weight 227000, butylated hydroxytoluene (BHT) and active ingredient (either mangostin or asiaticoside_ was suitable. From photoxidation study the result revealed that the percent contents of both mangostin and asiaticoside in formulation using BHT as antioxidant were slightly changed with in acceptablelimit. In addition, after 4 months of stability study at 30 degree Celsius the percent contents of mangostin and asiaticoside in formulation seem to be unchanged at various storage time intervals
DOI: 10.14457/cu.the.2006.2089
title: Preparation, characterization and application of titanium dioxide/activated carbon composite in 2-chlorophenol removal

description: TiO₂/AC composite nanoparticles in this work were prepared from alkoxide solution via sol-gel method. TiO₂/AC composite with different molar ratios (1:0, 1:10, 1:33, 1:50, 1:70, and 1:100) were determined. It was found that the ratios of TiO2: AC at 1:0 and 1:10 have anatase as predominant phase, while rutile phase appeared at ratio 1:33. Other types of TiO₂, TiO₂/O₂ and TiO₂/P-25 were also investigated in this work as well. In the adsorption isotherm part, it was found that TiO₂/O₂ was followed Langmuir adsorption isotherm, while TiO₂/AC/N₂ and TiO₂/N₂ were followed Freundlich adsorption isotherm. In photocatalytic process, the 2-chlorophenol removal efficiency can be ordered as TiO₂/AC/N₂ &gt; TiO₂/N₂ &gt; TiO₂/O₂ &gt; TiO₂/P-25. In the determination of effects of different calcination temperature (500ºC, 800ºC, 1100ºC, and 1300ºC) on the properties of TiO₂/AC. It was found that the calcinations temperature at 500ºC provided the most appropriate properties of TiO₂/AC for 2-chlorophenol removal. In a study of 2-chlorophenol degradation using TOC and GC/MS, no intermediates were detected. As the TOC appeared during the degradation process solely come from 2-chlorophenol, it can be concluded that the mineralization of 2-chlorophenol is achievable under TiO₂/AC process.
DOI: 10.14457/cu.the.2006.2090
title: The association between single nucleotide polymorphism within vascular endothelial growth factor gene with disease susceptibility and severity of psoriasis in Thai population
DOI: 10.14457/cu.the.2006.2091
title: Isolation and characterization of 4-chloroaniline-degrading bacteria

description: Chloroanilines have been widely used in several industries such as insecticides and herbicides etc. It is also accumulated in the environment owing to the microbial degradation of various types of chemical substances, namely, herbicides, phenyl urea, acylanilide, and phenylcarbamate. Three bacterial strains enabled to degrade 4CA were isolated from Thailand agricultural soil. they were identified based on the basis of morphology, biochemical characteristics and comparison of 16S rDNA sequence. These strains wer Acinetobacter baumannii, Pseudomonas putida, and Klebsiella pneumoniae. These bacteria showed the highest degradation rate and exhibited the percentage of 4-chloroaniline biodegradation were 61.00%±1.68, 59.82%±1.68, 59.82%±0.71, and 62.82%±3.87, respectively at 25 ppm (0.2 mM) of 4-chloroaniline which incubated for 12 days at 30℃. Furthermore, the dechlorination was monitored for the 4-chloroaniline degradation. Acinetobacter baumannii, Pseudomonas putida, and Klebsiella pneumoniae showed high chlorocatechol 1, 2 dioxygenase activity and fair activity of catechol 1,2 dioxegenase. Thesefore, the biodegradation of 4-chloroaniline by these three isolates was occured via the modified ortho-pathway. Moreover, the additional carbon or/and nitrogen source to enhance 4-chloroaniline degradation are, for Acinetobacter baumannii: 4mM citrate, 4mM NH₄Cl and 4mM citrate + 4mM NH₄Cl; for Pseudomonas putida: 4mM citrate and 4mM succinate, for Klebsiella pneumoniae: 1mM aniline and 2mM aniline. Furthermore, all isolates could degrade aniline, 2-chloroaniline, 3-chloroaniline but not degrade 3,4-dichloroaniline.
DOI: 10.14457/cu.the.2006.2092
title: การพัฒนาตัวบ่งชี้สมรรถนะของผู้บริหารสถานศึกษาของรัฐระดับมัธยมศึกษา สังกัดสำนักงานคณะกรรมการการศึกษาขั้นพื้นฐาน

description: การวิจัยนี้มีวัตถุประสงค์เพื่อ 1) พัฒนาตัวบ่งชี้สมรรถนะผู้บริหารสถานศึกษาของรัฐที่จัดการเรียนรู้ในระดับมัธยมศึกษา สังกัดสำนักงานคณะกรรมการการศึกษาขั้นพื้นฐาน โดยพิจารณาจากมาตรฐานตำแหนงและมาตรฐานวิทยฐานะและบุคลิกลักษณะของผู้บริหารสถานศึกษา และ 2) สำรวจสมรรถนะผู้บริหารสถานศึกษาของรัฐที่จัดการเรียนรู้ในระดับมัธยมศึกษาสังกัดสำนักงานคณะกรรมการการศึกษาขั้นพื้นฐาน จำแนกตามขนาดสถานศึกษาและภูมิภาค ตามการรับรู้ของผู้เกี่ยวข้อง กลุ่มตัวอย่างคือผู้บริหารสถานศึกษาของรัฐ ระดับมัธยมศึกษาสังกัดสำนักงานคณะกรรมการการศึกษาขั้นพื้นฐาน จำนวน 406 คน การเก็บรวบข้อมูลโดยใช้แบบสอบถามรองผู้อำนวยการและหัวหน้างานแผนงาน สถานศึกษาละ 2 คน การวิเคราะห์ข้อมูลใช้สถิติ เชิงบรรยายโดยการหาค่าเฉลี่ย ส่วนเบี่ยงเบนมาตรฐาน และการวิเคราะห์ความแปรปรวนแบบทางเดียว (one-Way ANOVA) ผลการวิจัยสรุปได้ดังนี้ 1. ผลการพัฒนาตัวบ่งชี้ได้ตัวบ่งชี้สมรรถนะผู้บริหารระดับมัธยมศึกษาจำนวน 78 ตัวบ่งชี้เป็นตัวบ่งชี้ด้านความรู้ 26 ด้านทักษะ 26 และด้านบุคลิกลักษณะ 26 ตัวบ่งชี้ พัฒนาจากร่างตัวบ่งชี้ที่มีค่าเฉลี่ยความสำคัญอยู่ระหว่าง 4.12-4.87 และมีคา IOC อยู่ระหว่าง 0.8-1.00 2. ผลการสำรวจสมรรถนะของผู้บริหารสถานศึกษาของรัฐระดับมัธยมศึกษา สังกัดสำนักงานคณะกรรมการการศึกษาขั้นพื้นฐาน พบว่า มีสมรรถนะในระดับสูงทั้งด้านความรู้ ทักษะและบุคลิกลักษณะ โดยมีผลการสำรวจสูงสุด้านความรู้ รองลงมาด้านบุคลิกลักษณะ และด้านทักษะตามลำดับ สมรรถนะที่มีผลการสำรวจสูงสุด คือ ทักษะในการติดต่อสื่อสาร และต่ำสุดคือทักษะการสั่งการ/การมอบหมายงานผลการสำรวจจำแนกตามขนาดโรงเรียนพบว่าโรงเรียนขนาดใหญ่พิเศษมีผลการสำรวจสูงสุดทั้ง 3 ด้าน รองลงมาเป็นโรงเรียนขนาดเล็ก ขนาดกลางและขนาดใหญ่ตามลำดับ ผู้บริหารโรงเรียนขนาดใหญ่พิเศษ ขนาดใหญ่ ขนาดกลางและขนาดเล็ก มีสมรรถนะสูงสุดคือความรู้ในเรื่องการประชาสัมพันธ์ การบริหารบุคคลและทีมงาน ความรับผิดชอบและ วิสัยทัศน์ ตามลำดับ ผลการสำรวจจำแนกตามภูมิภาค พบว่าภาคตะวันออกมีผลการสำรวจสูงสุดทั้ง 3 ด้าน รองลงมาเป็นภาคใต้ ภาคเหนือ ภาคตะวันออกเฉียงเหนือ ภาคตะวันออกเฉียงเหนือ ภาคกลาง และกรุงเทพมหานคร ตามลำดับ ผู้บริหารกรุงเทพมหานคร ภาคเหนือ ภาคกลาง ภาคตะวันออก ภาคตะวันออกเฉียงเหนือและภาคใต้มีสรรถนะสูงสุด ด้านความรู้ในเรื่องการประชาสัมพันธ์ และด้านบุคลิกลักษณะ ได้แก่ มีวิสัยทัศน์ ความรับผิดชอบ การอุทิศตนและเวลา และใจกว้างรับฟังความคิดเห็นของผู้อื่น ตามลำดับ
DOI: 10.25442/hku.12814649
title: Blackman-Esterling-Berk based FCS-CPA method in off-diagonal disorder systems

description: This dataset consists of the following parts: (1) the Python code to calculate the research data displayed in the thesis. (2) The calculation result used in the thesis (in .npy files). (3) The figures used to generate the thesis pdf file.
DOI: 10.25442/hku.12814649.v1
title: Blackman-Esterling-Berk based FCS-CPA method in off-diagonal disorder systems

description: This dataset consists of the following parts: (1) the Python code to calculate the research data displayed in the thesis. (2) The calculation result used in the thesis (in .npy files). (3) The figures used to generate the thesis pdf file.
DOI: 10.14457/cu.the.2006.2093
title: To study the associations between endogenous DNA double-strand breaks, DNA methylation, and cell cycle

description: This study aimed to evaluate the association between global hypomethylation and endogenous DSBs (EDSBs). Global hypomethylation is one of the most common molecular events in the multistep carcinogenesis. This process leads to genomic instability. EDSBs could account for a substantial fraction of oncogenic events in human carcinomas If EDSBs do not arise uniformly or are not processed at equal rates across the genome; mutation hot spots should be present. Therefore, genome wide methylation depletion may cause genomic instability if DNA methylation influences EDSB processes. In this thesis, we first developed a set of novel techniques to analyze the extent and methylation level of genomic EDSBs. The first is L1-EDSB-LMPCR and COBRA-L1-EDSB to quantitate level and methylation of EDSBs. The other is chromatin immunoprecipitating γ-H2AX, one of the earliest DSB repair responses. Then we evaluated the relation between quantity and methylation of EDSBs and the cellular DSB repair response. Our study discovered first endogenous DNA breakages are universally detectable and are hypermethylated. Second, the levels of EDSBs and γ-H2AX-bound DNA are cell type specific and inversely related. Whereas WBCs possessed more L1-EDSB-LMPCR than did epithelial cells, epithelial had more γ-H2AX-bound L1s than WBCs. Finally, the EDSBs are concealed in heterochromatin, which obstructs the early cellular DSB repair response. Trichostatin A treatment, which inhibits histone deacetylase and disrupts heterochromatin, released methylated EDSBs and increased the quantity and methylation level of γ-H2AX-bound DNA. DNA hypomethylation can therefore be linked to a high mutation rate if the early repair response of unmethylated EDSBs is error prone.
DOI: 10.14457/cu.the.2006.2094
title: Role of opioid receptors in sweet food intake in rats exposed to chronic restraint stress

description: The present study aimed to investigate the roles of the endogenous opioid system and its receptors (OR) in the development of sweet food intake in rats exposed to chronic restraint stress. The sweet food intake behavioral paradigm and the measurement of dopaminergic neurotransmitter levels in mesocorticolimbic system of rat treated with or without opioid receptor blockers were assessed. Male adult Wistar rats were divided into two groups: control (CON) and stress (ST) which rat were restrain 1 h/day, for 14 days. Both groups were subdivided into two groups; treated with or without non-selective or selective µ-, δ- or k-OR blockers (naloxone; NX, CTOP, naltridole; NT or nor-binaltorphimine dihydrochloride; nor-BNI, 0.5-1 mg/ml, s.c., 30 min before the bahavioral test). In the sweet food intake test, the rats were placed in the cage and allowed to consume sweet food (Froot loops® pellets), the number of ingested pellets during a period of 6 min was then recorded. Sweet food consumption was increased in chronic restraint stress-rats compared to control rats. Even though, there were no differences in the consumption of standard rat chow between groups. Adrenal gland mass associated with serum corticosterone level significantly increased in restraint stress-rats. These changes of adrenal gland mass and serum corticosterone confirmed the effective of stress model. Interestingly, the increase of sweet food consumption induced by restraint stress was inhibited by non-selective OR blocker (NX, 0.5 mg/ml) but not by any of selective OR blockers. To assess dopaminergic system, brain dopamine (DA) and its metabolites (DOPAC or HVA) level in amygdala, frontal cortex, hippocampus, and nucleus accumbens were measured by HPLC-EC. The dopamine levels were significntly increased in nucleus accumbens of chronic stressed-rat. Moreover, the sweet food consumption tended to increase the DA levels in nucleus accumbens in both stress and non-stress rats. On the other hand, dopaminergic activity (DOPAC/DA and HVA/DA ratios) of chronic stress-rats was lower than control rats. Additionally, the HVA levels in the hippocampus and the dopaminergic activity, shown by DOPAC/DA and HVA/DA ratios in the amygdala were significantly decreased in chronic stressed-rats. Further, nor-BNI tended to decrease dopamine levels in control rats, while other opioid antagonist did not affect. In contrast, naltridole could suppress DA level in nucleus accumbens in chronic stress rats while nor-BNI has no effect. These results suggested that chronic stress causes an increase in sweet food intake, and opioid system partially regulated stress induced-sweet food intake. Moreover, chronic stress may cause adaptation of δ- and k-OR in opioid systems as they differentially affected dopaminergic activity in mesolimbic system following OR antagonists administration. Nevertheless, this study has established the relationship between stress and opioid system in modulating mesolimbic dopamine system that affected sweet food intake.
DOI: 10.14457/cu.the.2006.2095
title: Carbofuran removal from soil by supplementation with agricultural residues

description: This research investigated the effects of organic amendments i.e., rice straw (RS), cassava pulp (CS), cattle manure (CM), compost (CP), corn cob (CC), glucose (GL), and molasses (ML) and inorganic amendment on removal of carbofuran from soil and aged soil. Initial carbofuran concentration at 50 mg/kg soil was spiked into soil microcosm. Results showed that 1.5% RS effectively stimulated indigenous microorganisms indicating by the shortest t1/2 of carbofuran of 7 days and high microbial activity. CP and CM were found to inhibit microbial activity suggesting by a decrease in dehydrogenase activity and a low carbofuran degradation. GL and ML at high load of 1.0% to 5.0% were more favorable to be used as C-source for indigenous microorganisms, therefore there was low carbofuran degraded in soil amended with GL and ML but there was high microbial activity. Inorganic amendment was found to inhibit carbofuran degradation as well as microbial activities in soil with t1/2 of 20-21 days. Addition of 1.5% RS or 1 mL of inorganic amendment into aged soil improved carbofuran degradation ability and microbial activities of indigenous microorganisms. Growth kinetic analysis of carbofuran degraders obtained from 1.5% RS stimulated soil indicated that carbofuran concentration in the contaminated site should be lower than 17.88 mg/L which corresponds to 13.23 L/kg soil, base upon Kd value of 0.74 in our soil sample, in order to obtain an effective biostimulation process by indigenous carbofuran degraders in soil sample used in this study.
DOI: 10.14457/cu.the.2006.2096
title: Leachability of glyphosate herbicide

description: The main objective of this study was to investigate the leaching behavior of glyphosate and its metabolite aminomethyphosphonic acid (AMPA) with and without the influence of the chemical fertilizer N-P-K [in the ratio of 16:16:16] under rainy season and summer conditions. The specific effects of the chemical fertilizer on the accumulation of glyphosate and its metabolite aminomethyphosphonic acid in soil were determined. The experiment was performed under outdoor conditions by using soil columns. The leaching of glyphosate was monitored for 60 days, after that the residual glyphosate in the soil was evaluated. The amounts of glyphosate leaching influenced by the chemical fertilizer were clearly higher than the leaching that occurred without the influence of the chemical fertilizer. The total amounts of glyphosate leaching influenced by the chemical fertilizer were 6.53 % and 1.92% for rainy season and summer, respectively, while the total amount of glyphosate leaching in the soil columns that were not influenced by the chemical fertilizer was 0.03 % during both the rainy season and summer. The total AMPA leaching from the experimental groups were 2.77% and 1.82 % in the rainy season and summer, while the total AMPA in the rainy control group was 0.09%, the lowest result. In soil, the total accumulation levels of glyphosate influenced by the chemical fertilizer were 6.30 % and 2.39 % for the rainy season and summer, respectively, while the total accumulation of glyphosate when not influenced by the chemical fertilizer was 0.43%. For AMPA, the total accumulation levels of AMPA influenced by the chemical fertilizer were 7.35 % and 7.30 % for rainy season and summer, respectively, while the total accumulation of AMPA when not influenced by the chemical fertilizer was 1.77 %. From the results, the chemical fertilizer was seen to affect the behavior of both glyphosate leaching and accumulation in soil. The phosphorus contained in the chemical fertilizer replaced glyphosate in specific sorption sites by the competition mechanism. The replaced glyphosate then had the opportunity to form complexes with Ca and Mg. Consequently, its complexation was more difficult for the soil microbial organisms to degrade. The amounts of AMPA both in the leachate and soil were found to be related to the amount of glyphosate that remained after undergoing degradation.
DOI: 10.14457/cu.the.2006.2097
title: Effects of Terminalia Citrina Roxb. alcoholic extract on extended spectrum beta-lactamase producing Escherichia Coli

description: Extended spectrum beta-lactamase producing Escherichia coli is a problem in hospitalized patients worldwide and are increasingly associated with community acquired infection. The most frequent coresistances found in ESBL-producing organisms are broad spectrum beta-lactam, third generation cephalosporins, aminoglycosides and fluoroquinolones. Combination therapy is the other choice in the treatment of ESBLproducing E.coli infections with the aim of decreasing the emergence of resistance strains and increasing bacterial killing. Therefore, the purpose of the present study is to determine the susceptibility of alcoholic extract of T. citina ROXB, ampicillin and norfloxacin against 30 E.coli ( 29 ESBL-producing,1 non- ESBL-producing) strains. The MIC90 values were 10 mg/ml, &gt;256 μg/ml and 64 μg/ml, respectively. All of 30 strains were beta-lactamase positive by Nitrocefin base test. The high ampicillin resistance were detected (MIC range 256- &gt;256 μg/ml). There were 18 strains of ESBL –producing E.coli (60%), which were resistant to both ampicillin and norfloxacin. Checkerboard method served to determine the activity of extract in combination with ampcillin and norfloxacin. Combination of the extract with ampicillin showed indifference against 30 E.coli strains while norfloxacin plus the extract against 18 strains of ESBL –producing E.coli (resistance to both ampicillin and norfloxacin) showed synergistic effect in 11 strains (61.11%), partial synergistic effect in 6 strains (33.33%) and additive effect in 1 strain (5.55%). In the Time kill study using 18 ESBL-producing E.coli strains, extract alone (½ MIC, 1 MIC) showed bacteriostatic activity (90% killing), whereas norfloxacin alone (½ MIC) showed bactericidal activity (99.9% killing) in 2 strains at 8 hour of growth. However, the regrowth were observed in 14, 16 and 12 strains at 24 hour by the extract alone or norfloxacin alone. When the concentration of the extract was increased from ½ MIC to 1 MIC and combined with norfloxacin, the bactericidal rate was faster and the number of strains killed was increased. For example, in strain no. U16 had the bactericidal activity was seen at 2 hour instead of 4 hour when using the conbination of the extract 1 MIC plus norfloxacin. At 24 hour of growth, the bacteriostatic and bactericidal activity (90-99.9% killing ) were observed in only 4 strains [BA24=164.75 ±30.69 log CFU/ml·h] (1/2 MIC of the extract plus norfloxacin), while conbined 1 MIC of the extract plus norfloxacin showed the bacteriostatic and bactericidal activity (90-99.9% killing) against 16 strains [BA24=175.78±30.41 log CFU/ml·h]. The results obtained suggested that antibacterial activity of the combination extract plus norfloxacin were higher than the antibacterial activity of each drug. It is concluded that the combination of extract plus norfloxacin could be promising alternatives in the treatment of infections due to ESBL-producing E.coli that were resistant to ampicillin and norfloxacin.