cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30127210
title: Transgenic barley over-expressing <i>Aspergillus niger</i> phytase phyA in field trials

description: Phytic acid is the main storage of phosphate in grains of staple crops. As phytic acid is hardly digestible for non-ruminants microbial phytases are used to supplement animal feed to enhance phosphate digestibility. A fungal phytase gene was introduced into barley with the aim of enhancing phosphate digestibility. Transgenic homozygous barley over-expressing fungal phytase phyA showed a 3.3fold increase in mature grain phytase activity. Field trials at two locations in the Czech Republic were conducted in a five-year experiment to test transgene stability and activity under field conditions. Increased phytase activity gradually decreased over the generations showing the most significant drop in the initial years of field trials. Molecular analysis revealed methylation in the coding sequence of the <i>phyA</i> transgene, suggesting transcription gene silencing. On the other hand, herbicide resistance used for selection of transgenic plants was functional over all generations. The feasibility of crossing the transgene into the feeding cultivar Azit was demonstrated with subsequent stabilization of hybrid progeny through androgenesis. Our results indicate that the Azit genetic background tended to reduce phytase activity in mature grains of hybrids. Grain-specific over-expression of fungal phytase driven by an amylase promoter improved phosphate levels during germination. Unfortunately, a malting experiment revealed that phytase over-expression did not significantly improve malting parameters. In fact, the higher nitrogen content in unmalted grain negatively affected the quality of the malt produced from them.
DOI: 10.6084/m9.figshare.30127210.v1
title: Transgenic barley over-expressing <i>Aspergillus niger</i> phytase phyA in field trials

description: Phytic acid is the main storage of phosphate in grains of staple crops. As phytic acid is hardly digestible for non-ruminants microbial phytases are used to supplement animal feed to enhance phosphate digestibility. A fungal phytase gene was introduced into barley with the aim of enhancing phosphate digestibility. Transgenic homozygous barley over-expressing fungal phytase phyA showed a 3.3fold increase in mature grain phytase activity. Field trials at two locations in the Czech Republic were conducted in a five-year experiment to test transgene stability and activity under field conditions. Increased phytase activity gradually decreased over the generations showing the most significant drop in the initial years of field trials. Molecular analysis revealed methylation in the coding sequence of the <i>phyA</i> transgene, suggesting transcription gene silencing. On the other hand, herbicide resistance used for selection of transgenic plants was functional over all generations. The feasibility of crossing the transgene into the feeding cultivar Azit was demonstrated with subsequent stabilization of hybrid progeny through androgenesis. Our results indicate that the Azit genetic background tended to reduce phytase activity in mature grains of hybrids. Grain-specific over-expression of fungal phytase driven by an amylase promoter improved phosphate levels during germination. Unfortunately, a malting experiment revealed that phytase over-expression did not significantly improve malting parameters. In fact, the higher nitrogen content in unmalted grain negatively affected the quality of the malt produced from them.
DOI: 10.6084/m9.figshare.29370077
title: Initiating the commercialization of genetically modified staple crops in China: domestic biotechnological advancements, regulatory milestones, and governance frameworks

description: Historically, China’s approach to genetically modified (GM) staple crops has been cautious. However, in 2021, China launched its first pilot program for the commercial cultivation of GM food crops and subsequently expanded their cultivation. Previously, the only GM plants cultivated in China were insect-resistant cotton and virus-resistant papaya. The regulatory and policy shifts, from initial research and cautious development to accelerated commercialization, led to significant changes in China’s safety-related regulatory framework for genetically modified organism (GMOs). Here, China’s progress in GMO research and commercialization over the past three decades is comprehensively analyzed. This review traces the evolution of core regulations governing GMO safety, summarizes the Chinese model of GMO safety governance, and highlights the remaining challenges as commercialization progresses. The goal was to present the international community with a GMO safety governance model reflecting China’s characteristics and practices, offering a Chinese solution to balancing GMO commercial adoption with ecological preservation.
DOI: 10.6084/m9.figshare.29370077.v2
title: Initiating the commercialization of genetically modified staple crops in China: domestic biotechnological advancements, regulatory milestones, and governance frameworks

description: Historically, China’s approach to genetically modified (GM) staple crops has been cautious. However, in 2021, China launched its first pilot program for the commercial cultivation of GM food crops and subsequently expanded their cultivation. Previously, the only GM plants cultivated in China were insect-resistant cotton and virus-resistant papaya. The regulatory and policy shifts, from initial research and cautious development to accelerated commercialization, led to significant changes in China’s safety-related regulatory framework for genetically modified organism (GMOs). Here, China’s progress in GMO research and commercialization over the past three decades is comprehensively analyzed. This review traces the evolution of core regulations governing GMO safety, summarizes the Chinese model of GMO safety governance, and highlights the remaining challenges as commercialization progresses. The goal was to present the international community with a GMO safety governance model reflecting China’s characteristics and practices, offering a Chinese solution to balancing GMO commercial adoption with ecological preservation.
DOI: 10.15468/ssy4eb
title: Cortinarius kashmirensis sp. nov. in Cortinarius sect. Disjungendi (Agaricales) from Pakistan

description: This dataset contains the digitized treatments in Plazi based on the original journal article Khurshid, Rubab, Naseer, Arooj, Garrido-Benavent, Isaac, Khalid, Abdul Nasir (2023): Cortinarius kashmirensis sp. nov. in Cortinarius sect. Disjungendi (Agaricales) from Pakistan. Phytotaxa 583 (1): 50-60, DOI: 10.11646/phytotaxa.583.1.5, URL: http://dx.doi.org/10.11646/phytotaxa.583.1.5AbstractDuring recent fieldwork at Azad Jammu and Kashmir (Pakistan), many basidiomycetes of the genus Cortinarius were collected from mixed coniferous Himalayan temperate forests. Their morphological characterization and phylogenetic analyses based on ITS and LSU sequence data yielded a well-delimited, new species within C. subgenus Telamonia sect Disjungendi. It is characterized by producing small and dry basidiomata with blackish brown conico-convex pilei when young and uplifted at maturity, dark brown lamellae, a fibrillose stipe and small basidiospores (9.4 × 5.5 µm in average).
DOI: 10.6084/m9.figshare.21286313
title: Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

description: Genome editing tools have rapidly been adopted by plant scientists for crop improvement. Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful technique for crop improvement in monocot species. In this study, we utilized precise gene editing techniques to generate wheat 3’(2’), 5’-bisphosphate nucleotidase (<i>TaSal1</i>) mutants using a multiplex sgRNA-CRISPR/Cas9 genome editing system. Five active <i>TaSal1</i> homologous genes were found in the genome of Giza168 in addition to another apparently inactive gene on chromosome 4A. Three gRNAs were designed and used to target exons 4, 5 and 7 of the five wheat <i>TaSal1</i> genes. Among the 120 Giza168 transgenic plants, 41 lines exhibited mutations and produced heritable <i>TaSal1</i> mutations in the M<sub>1</sub> progeny and 5 lines were full 5 gene knock-outs. These mutant plants exhibit a rolled-leaf phenotype in young leaves and bended stems, but there were no significant changes in the internode length and width, leaf morphology, and stem shape. Anatomical and scanning electron microscope studies of the young leaves of mutated <i>TaSal1</i> lines showed closed stomata, increased stomata width and increase in the size of the bulliform cells. <i>Sal1</i> mutant seedlings germinated and grew better on media containing polyethylene glycol than wildtype seedlings. Our results indicate that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing is efficient tool for mutating more multiple TaSal1 loci in hexaploid wheat.
DOI: 10.6084/m9.figshare.21286313.v1
title: Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

description: Genome editing tools have rapidly been adopted by plant scientists for crop improvement. Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful technique for crop improvement in monocot species. In this study, we utilized precise gene editing techniques to generate wheat 3’(2’), 5’-bisphosphate nucleotidase (<i>TaSal1</i>) mutants using a multiplex sgRNA-CRISPR/Cas9 genome editing system. Five active <i>TaSal1</i> homologous genes were found in the genome of Giza168 in addition to another apparently inactive gene on chromosome 4A. Three gRNAs were designed and used to target exons 4, 5 and 7 of the five wheat <i>TaSal1</i> genes. Among the 120 Giza168 transgenic plants, 41 lines exhibited mutations and produced heritable <i>TaSal1</i> mutations in the M<sub>1</sub> progeny and 5 lines were full 5 gene knock-outs. These mutant plants exhibit a rolled-leaf phenotype in young leaves and bended stems, but there were no significant changes in the internode length and width, leaf morphology, and stem shape. Anatomical and scanning electron microscope studies of the young leaves of mutated <i>TaSal1</i> lines showed closed stomata, increased stomata width and increase in the size of the bulliform cells. <i>Sal1</i> mutant seedlings germinated and grew better on media containing polyethylene glycol than wildtype seedlings. Our results indicate that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing is efficient tool for mutating more multiple TaSal1 loci in hexaploid wheat.
DOI: 10.6084/m9.figshare.29370077.v1
title: Initiating the commercialization of genetically modified staple crops in China: domestic biotechnological advancements, regulatory milestones, and governance frameworks

description: Historically, China’s approach to genetically modified (GM) staple crops has been cautious. However, in 2021, China launched its first pilot program for the commercial cultivation of GM food crops and subsequently expanded their cultivation. Previously, the only GM plants cultivated in China were insect-resistant cotton and virus-resistant papaya. The regulatory and policy shifts, from initial research and cautious development to accelerated commercialization, led to significant changes in China’s safety-related regulatory framework for genetically modified organism (GMOs). Here, China’s progress in GMO research and commercialization over the past three decades is comprehensively analyzed. This review traces the evolution of core regulations governing GMO safety, summarizes the Chinese model of GMO safety governance, and highlights the remaining challenges as commercialization progresses. The goal was to present the international community with a GMO safety governance model reflecting China’s characteristics and practices, offering a Chinese solution to balancing GMO commercial adoption with ecological preservation.
DOI: 10.15468/krd7w2
title: Pleurothallis velosana (Orchidaceae: Pleurothallidinae), a new species from the eastern Andes of Colombia

description: This dataset contains the digitized treatments in Plazi based on the original journal article Sierra-Ariza, Mario Alexei, Molina, Jhon Fredy Rojas, Wilson, Mark (2025): Pleurothallis velosana (Orchidaceae: Pleurothallidinae), a new species from the eastern Andes of Colombia. Phytotaxa 715 (2): 164-170, DOI: 10.11646/phytotaxa.715.2.7, URL: https://doi.org/10.11646/phytotaxa.715.2.7
DOI: 10.15468/ppzh2s
title: Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

description: This dataset contains the digitized treatments in Plazi based on the original journal article Fan, Sophia L., Carle, Danielle De, Kvist, Sebastian (2022): Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada. Zootaxa 5159 (2): 265-280, DOI: 10.11646/zootaxa.5159.2.5AbstractThe leech genus Placobdella harbours 24 currently-recognized species, and only a handful of new species has been described in the past decade. Placobdella akahkway n. sp., a new species of ectoparasitic glossiphoniid leech is herein described from sites in the provinces of Alberta, Saskatchewan, and Manitoba, Canada. Beyond morphological description of the species, a total of 21 specimens (including the five specimens from the type series) were also employed in a phylogenetic analysis using the COI locus. The new species can be distinguished from its congeners by its possession of both compact and diffuse salivary glands. The included specimens of Placobdella akahkway n. sp. form a monophyletic group with high support, and place as the sister taxon to Placobdella kwetlumye.
DOI: 10.15468/bb4e43
title: Dionysia persica (Primulaceae), a new species from Shiraz, Iran

description: This dataset contains the digitized treatments in Plazi based on the original journal article Alipour, Sajad, Majidi, Ramiar, Jalilian, Nastaran (2025): Dionysia persica (Primulaceae), a new species from Shiraz, Iran. Phytotaxa 694 (1): 86-92, DOI: 10.11646/phytotaxa.694.1.7, URL: https://doi.org/10.11646/phytotaxa.694.1.7AbstractA new species, Dionysia persica Majidi & Alipour (Primulaceae) from Shiraz, Iran, is described and illustrated. It is similar to Dionysia bokharii but differs in leaves with usually deeply lobed and pinnately veined with weakly sclerified veins, much shorter calyx and corolla tube, smaller corolla limb with narrower and more distinctly emarginate lobes, and fewer ovules and seeds. Unlike all other Iranian Dionysia species, it is homostylous. A detailed description, photographs, geographical distribution, conservation assessment, and taxonomic comments on the new species are presented.
DOI: 10.15468/fhckyw
title: A New Species of Phanaeus Macleay (Coleoptera: Scarabaeidae, Scarabaeinae) from Sonora, Mexico

description: This dataset contains the digitized treatments in Plazi based on the original journal article Edmonds, W. D. (2004): A New Species of Phanaeus Macleay (Coleoptera: Scarabaeidae, Scarabaeinae) from Sonora, Mexico. The Coleopterists Bulletin 58 (1): 119-124, DOI: 10.1649/697, URL: http://dx.doi.org/10.1649/697
DOI: 10.15468/dl.4fha2d
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "MULTIPOLYGON (((149.063454 -36.479045, 149.063454 -36.485782, 149.065699 -36.485782, 149.065699 -36.490274, 149.072437 -36.490274, 149.072437 -36.494766, 149.074683 -36.494766, 149.074683 -36.485782, 149.079174 -36.485782, 149.079174 -36.490274, 149.08142 -36.490274, 149.08142 -36.485782, 149.085912 -36.485782, 149.085912 -36.490274, 149.106124 -36.490274, 149.106124 -36.476799, 149.103878 -36.476799, 149.103878 -36.470062, 149.101632 -36.470062, 149.101632 -36.467816, 149.090403 -36.467816, 149.090403 -36.472308, 149.088157 -36.472308, 149.088157 -36.476799, 149.065699 -36.476799, 149.065699 -36.479045, 149.063454 -36.479045), (149.08142 -36.479045, 149.085912 -36.479045, 149.085912 -36.483537, 149.08142 -36.483537, 149.08142 -36.479045)), ((149.063454 -36.479045, 149.063454 -36.476799, 149.056716 -36.476799, 149.056716 -36.479045, 149.063454 -36.479045)))"
 ]
}

 The dataset includes 8 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054729-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.26186/149293
title: Potential groundwater dependent ecosystems in the South Nicholson - Georgina Basin identified using Landsat satellite data

description: Groundwater dependent ecosystems (GDEs) rely on access to groundwater on a permanent or intermittent basis to meet some or all of their water requirements (Richardson et al., 2011). The Tasseled Cap percentile products created by Digital Earth Australia (2023) were used to identify potential GDEs for the South Nicholson-Georgina basins study area. These percentile products provide statistical summaries (10th, 50th, 90th percentiles) of landscape brightness, greenness and wetness in imagery acquired between 1987 and present day. The 10th percentile greenness and wetness represent the lowest 10% of values for the time period evaluated, e.g. 10th percentile greenness represents the least green period. In arid regions, areas that are depicted as persistently green and/or wet at the 10th percentile have the greatest potential to be GDEs. For this reason, and due to accessibility of the data, the 10th percentile Tasseled Cap greenness (TCG) and Tasseled Cap wetness (TCW) products were used as the basis for the assessment of GDEs for the South Nicholson-Georgina region. The 50th percentile greenness was utilised to create the coefficient of variance (CV) dataset.

This data release is an ESRI geodatabase, with layer files, including:
 
- combined classified 10th percentile greenness and wetness dataset (useful to identify potential groundwater dependent vegetation/other GDEs and differentiate between vegetation types)
 
- CV of 50th percentile greenness dataset (useful when used in conjunction with the combined product to help identify groundwater dependent vegetation)
 
For more information and detail on these products, refer to associated report.
<br><strong>References</strong>Digital Earth Australia (2023). <em>Digital Earth Australia User Guide.</em>Richardson, S., E. Irvine, R. Froend, P. Boon, S. Barber, and B. Bonneville. 2011a. <em>Australian groundwater-dependent ecosystem toolbox part 1: Assessment framework.</em> Waterlines Report 69. Canberra, Australia: Waterlines.<br>
DOI: 10.15468/dl.gmun46
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((148.818662 -36.49252, 148.818662 -36.494766, 148.827645 -36.494766, 148.827645 -36.49252, 148.818662 -36.49252))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054731-240626123714530/datasets/export for details.
DOI: 10.15468/dl.vyhap4
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.088157 -36.49252, 149.088157 -36.494766, 149.085912 -36.494766, 149.085912 -36.499257, 149.090403 -36.499257, 149.090403 -36.49252, 149.088157 -36.49252))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054732-240626123714530/datasets/export for details.
DOI: 10.25380/iastate.24061518
title: 3D analog and numerical experiments on grain comminution

description: This dataset contains data from analog and numerical experiments, both subsets investigate the comminution of grains under simple shear conditions. The analog subset contains photographs (.jpg) of 6 analog experiments performed in the ring shear apparatus designed at the Structure Lab of Iowa State University—taken before, halfway, and after the experiments. In addition, “CF_LG” contains a video (.mov) in real time (30 minute 30 seconds); “CF_D” contains a 52-second video (.mp4) that corresponds to 30 minute 30 seconds in real time. The numerical subset contains input files (.csv), scripts (.py, .prm), and videos (.avi) from 8 numerical experiments performed using the open-source Discrete Element code ESyS-Particle version 2.3.5. The numerical experiments are benchmarked by the analogy experiments, and each 25-second video corresponds to 15 minute 50 seconds in real time.
DOI: 10.25380/iastate.24061518.v1
title: 3D analog and numerical experiments on grain comminution

description: This dataset contains data from analog and numerical experiments, both subsets investigate the comminution of grains under simple shear conditions. The analog subset contains photographs (.jpg) of 6 analog experiments performed in the ring shear apparatus designed at the Structure Lab of Iowa State University—taken before, halfway, and after the experiments. In addition, “CF_LG” contains a video (.mov) in real time (30 minute 30 seconds); “CF_D” contains a 52-second video (.mp4) that corresponds to 30 minute 30 seconds in real time. The numerical subset contains input files (.csv), scripts (.py, .prm), and videos (.avi) from 8 numerical experiments performed using the open-source Discrete Element code ESyS-Particle version 2.3.5. The numerical experiments are benchmarked by the analogy experiments, and each 25-second video corresponds to 15 minute 50 seconds in real time.
DOI: 10.15468/dl.kr9p86
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.103878 -36.49252, 149.103878 -36.494766, 149.106124 -36.494766, 149.106124 -36.49252, 149.103878 -36.49252))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054733-240626123714530/datasets/export for details.
DOI: 10.17632/w5zspm82zd.1
title: Numismatic Heritage of Coin Dataset Bangladesh

description: Bangladesh introduced taka coins in 1973, featuring culturally significant designs, and updated the series in 2013 to include portraits of national figures and the national emblem. A dataset containing 9,983 images of these coins, across 26 different classes, aids in developing technologies for visually impaired individuals to facilitate their banking transactions. This work enhances coin recognition and integrity checks, utilizing computer vision and AI to improve accessibility in financial environments.
DOI: 10.15468/dl.kcf5xq
title: Occurrence Download

description: A dataset containing 3454 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is one of (Fremontodendron californicum (Torr.) Coult.)"
 ]
}

 The dataset includes 3454 records from 122 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054734-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.t3pgm4
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.12409 -36.494766, 149.12409 -36.499257, 149.126336 -36.499257, 149.126336 -36.494766, 149.12409 -36.494766))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054735-240626123714530/datasets/export for details.
DOI: 10.15468/dl.rvwqcf
title: Occurrence Download

description: A dataset containing 124 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Shorea robusta Gaertn.",
 "Year 1960-2024"
 ]
}

 The dataset includes 124 records from 14 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0054736-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.