DOI: 10.5061/dryad.8kprr4xvd
title: A review of Appalachian Dasycerus Brongniart, and the recognition of cryptic diversity within Dasycerus carolinensis Horn (Coleoptera: Staphylinidae: Dasycerinae)

description: ­Previous analyses have revealed deep divergences among populations of the
 relictual and enigmatic rove beetle, Dasycerus carolinensis Horn. New data
 from additional populations, molecular markers, and morphology
 unambiguously reveal this 'species' to represent a complex of
 closely related species, distinguishable by characters of the male
 genitalia, and corresponding closely to geographically coherent clades
 discovered by molecular analyses. Calibrated dating analyses show
 Appalachian Dasycerus to have been diverging in the region for more than
 10 million years, yet largely respecting important biogeographic barriers
 in the region, such as the French Broad and Little Tennessee River
 drainages. In addition to discussing finer scale biogeographic patterns in
 the group, we formally recognize 9 new species from within what was
 formerly known as D. carolinensis: D. virginiensis sp. nov., D. tuckasegee
 sp. nov., D. pacolet sp. nov., D. chattooga sp. nov., D. itseyi sp. nov.,
 D. unicoi sp. nov., D. nikwasi sp. nov., D. egwanulti sp. nov., and D.
 gadalutsi sp. nov.. It was not, however, possible to assign all samples to
 one of these species, and specimens from some sparsely sampled outlying
 areas, northern Alabama and central Tennessee in particular, may represent
 additional species. 
description: The data set comprises sequences from two regions of the
 mitochondrial COI gene and one of the Carbamoylphosphate synthase domain
 protein (CAD) gene, generated through PCR-based Sanger and Next-generation
 sequencing.
description: # A review of Appalachian Dasycerus Brongniart, and the recognition of
 cryptic diversity within Dasycerus carolinensis Horn (Coleoptera:
 Staphylinidae: Dasycerinae)
 [https://doi.org/10.5061/dryad.8kprr4xvd](https://doi.org/10.5061/dryad.8kprr4xvd) ## Description of the data and file structure This data set consists of one nexus-formatted file containing aligned sequences of two mitochondrial COI regions and one Carbamoylphosphate synthase domain protein (CAD) region for the staphylinid rove beetle genus *Dasycerus*. Altogether, the data set includes 198 taxa (including 2 non-Dasycerus outgroups) and 1667 nucleotide positions (COIa = 1 -421; COIb = 422 -1157; CAD = 1158 -1667). Nexus files may be opened in Mesquite, and analyzed in numerous phylogenetics software packages. ## Sharing/Access information The unaligned sequences may be accessed from GenBank with the following link: * [https://www.ncbi.nlm.nih.gov/nuccore/?term=Dasycerus+caterino](https://www.ncbi.nlm.nih.gov/nuccore/?term=Dasycerus+caterino) Data was derived from the following sources: * all sequences were generated by the Caterino Arthropod Biodiversity Lab, Clemson University * Some sequences were previously published in: Michael S Caterino, Shelley S Langton-Myers, Intraspecific Diversity and Phylogeography in Southern Appalachian *Dasycerus carolinensis* (Coleoptera: Staphylinidae: Dasycerinae), *Insect Systematics and Diversity*, Volume 3, Issue 6, November 2019, 8, [https://doi.org/10.1093/isd/ixz022](https://doi.org/10.1093/isd/ixz022)