DOI: 10.5061/dryad.ns1rn8q32
title: Soil microbe-induced plant volatiles can alert neighboring plants for tolerating heavy metal stress

description: Induced volatiles can elicit defense or tolerance responses in neighboring
 receiver plants, a phenomenon referred to as the ‘alerting effect’. While
 the alerting effect of microbe-induced plant volatiles (MIPVs) to biotic
 stressors has been extensively studied, the function of MIPVs in relation
 to abiotic stressors has received less attention. Furthermore, whether
 changes in MIPVs can prime neighboring plants in preparation for
 subsequent heavy metal stress remains unclear. This study employs
 Phytolacca americana as study material to evaluate the impact of manganese
 (Mn) stress on the emission of volatile organic compounds (VOCs) and
 MIPVs. Additionally, we investigate whether the changes in MIPVs released
 by the emitter plants contribute to Mn tolerance in neighboring receiver
 plants. Our findings demonstrated that elevated levels of Mn stress
 significantly increased the total emissions and changed the compositions
 of MIPVs from plants cultivated in non-sterilized soil, in contrast to
 that of VOCs emitted by plants in sterilized soil. Moreover, MIPVs under
 high Mn stress conditions notably altered the hormonal profiles of the
 receiver plants, leading to a modification in their CSR strategies.
 Consequently, the receiver plants exhibited enhanced tolerance to
 subsequent Mn stress, as evidenced by improved growth performance,
 increased antioxidant enzyme activity, and reduced membrane damage. This
 study represents one of the initial efforts to elucidate the alerting
 effect of MIPVs induced by heavy metal stress on neighboring plants and
 its ecological implications.
description: ## Soil microbe-induced plant volatiles can alert neighboring plants for
 tolerating heavy metal stress Under manganese stress, soil microorganisms
 induced changes in the volatile compounds of Phytolacca americana,
 triggering alerting effect in neighboring receiver plants. The production
 of MIPVs from emitter plants led receiver plants to shift their hormone
 state and ecological strategies, enhancing their tolerance to subsequent
 manganese stress. ## Description of the Data and file structure In this
 file, there are seven sheets, which include Experiments 1, 2, 3, and 4
 measured in this study. ### Experiment 1 In Experiment 1,there are two
 sheets, including concentration (ng/mL) of VOCs from emitter plants grown
 on sterilized soil and MIPVs of emitter plants grown on non-sterilized
 soil were listed. The column headers represent the CAS number of VOCs
 substance,VOCs that could not be retrieved are represented by unknown. ###
 Experiment 2 In Experiment 2, there are two sheets, the leaf SA level
 (mg/g FW), the SA contents in root exudate(ng/ml), the leaf IAA level(ng/g
 FW), and CSR strategy scores(%) of receiver plants were listed in one
 sheet; soil bacterial OTU relative abundance (%) of MIPVs emitter and
 receiver plants were listed in the other one. ### Experiment 3 In
 Experiment 3, the sheet includes dry weight (mg) (leaf, stem, root, and
 total) and CSR strategy scores(%) of receiver plants after Mn stress. ###
 Experiment 4 In Experiment 4, there are two sheets, including tolerance
 indicators of VOCs receiver plants and MIPVs receiver plants before or
 after Mn stress. The treatment codes are listed as below: (1)Emitter
 plants: Sterilized soil- inoculation with sterilized soil Non-sterilized
 soil- inoculation with non-sterilized soil e0mM-treatment with soil under
 non-Mn stress e10mM-treatment with soil under 10mM Mn stress
 e30mM-treatment with soil under 30mM Mn stress e50mM-treatment with soil
 under 50mM Mn stress (2)Receiver plants: Sterilized soil- treatment with
 VOCs from emitter plants in sterilized soil Non-sterilized soil- treatment
 with MIPVs from emitter plants in non-sterilized soil R0mM-treatment with
 volatiles from emitter plants under non-Mn stress R10mM-treatment with
 volatiles from emitter plants under 10mM Mn stress R30mM-treatment with
 volatiles from emitter plants under 30mM Mn stress R50mM-treatment with
 volatiles from emitter plants under 50mM Mn stress ### Abbreviations used
 as below: VOCs-volatile organic compounds MIPVs-microbe-induced plant
 volatiles SA-salicylic acid IAA-indole-3-acetic acid OTU-operational
 taxonomic units CSR-competitiveness (C), stress tolerance (S) and
 ruderality (R) CAT-catalase POD-peroxidase SOD-superoxide dismutase
 H2O2-hydrogen peroxide MG-methylglyoxal MDA-malondialdehyde ##
 Sharing/access Information Links to other publicly accessible locations of
 the data: No Was data derived from another source? No