DOI: 10.5061/dryad.bq570
title: Data from: Sexually antagonistic evolution caused by male-male competition in the pistil

description: While sexual selection and sexual conflict are important evolutionary
 forces in animals, their significance in plants is uncertain. In
 hermaphroditic organisms, such as many plants, sexual conflict may occur
 both between mating partners (inter-locus conflict) and between male and
 female sex roles within an individual (intra-locus conflict). We performed
 experimental evolution, involving lines that were crossed with either one
 or two pollen donors (monogamous or polyandrous lines), in the
 hermaphroditic plant (Collinsia heterophylla) where early fertilizations
 are associated with female fitness costs (reduced seed set). Artificial
 polyandry for four generations resulted in enhanced pollen performance and
 increased female fitness costs compared to the monogamous and source
 (starting material) lines. Female fitness was also reduced in the
 monogamous line, indicating a possible trade-off between sex roles,
 resulting from early pollination. We performed a second experiment to
 investigate a potential harming effect of pollen performance on seed set.
 We found that high siring success of early-arriving pollen competing with
 later-arriving pollen was associated with high female fitness costs,
 consistent with an inter-locus sexual conflict. Our study provides
 evidence for the importance of sexual selection in shaping evolution of
 plant reproductive strategies, but also pinpoints the complexity of sexual
 conflict in hermaphroditic species.
description: Data_seed
 germination_experiment 1Data on proportion
 germinated seeds in experimental evolution lines monogamous M, polyandrous
 P, and source S in Collinsia heterophylla.Data_flower
 production_experiment 1Data on flower
 production in experimental evolution lines monogamous M, polyandrous P,
 and source S in Collinsia heterophylla. Flower production estimated as
 number of branches times number of flowers in top
 branch.Data_pollen tube growth
 rate_experiment 1Data on pollen tube
 growth rate in experimental evolution lines monogamous M, polyandrous P,
 and source S in Collinsia heterophylla. Pollen tube growth rate estimated
 in vitro as a mean for ten replicate pollen tubes after 1h, 45
 min.Data_seed
 production_experiment 1Data on seed production
 in experimental evolution lines monogamous M, polyandrous P, and source S
 in Collinsia heterophylla following one-donor crosses within and between
 lines at floral developmental stage 1-4. Seed production estimated as
 number of seeds per capsule.Data_siring success and
 seed production_experiment 2Data on siring success
 of a focal pollen donor and seed production in Collinsia heterophylla
 following two-donor crosses involving a focal pollen donor and a standard
 competing pollen donor on a set of recipient plants. Pollen from the focal
 pollen donor was applied at floral developmental stage 1 and pollen from
 the standard competitor was applied at floral developmental stage 2, i.e.
 one day later. Siring success estimated as proportion seeds sired by the
 donors using microsatellite markers in up to 20 seeds from four replicate
 crosses using the same focal and standard pollen donors, and the same
 recipient. Seed production estimated as number of seeds per capsule as a
 mean of the four replicate crosses.