cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9599486
title: Richness and endemism of Lauraceae in Paraná: Phytogeographical aspects and priority areas for conservation

description: Abstract Lauraceae are among the three main families of the arboreal component of the forests in Paraná, being a frequent element in phytosociological and biogeographic studies. In spite of this, the family remains insufficiently known in the state. Our aims were: to recognize the species of Lauraceae in Paraná, relating them to the phytogeographic units; map the regions with the greatest species richness and concentration of endemisms; identify complementarity areas and compare them with the priority areas for conservation. We used collection records from 23 national herbaria, totaling 3,332 specimens analyzed, plotting their geographic coordinates onto the phytogeographic map of the state. We found that there are 76 species in Paraná, of which eleven are cited for the first time in the state. The Atlantic Rainforest contains the largest number of species (59 spp.), followed by the Araucaria Rainforest (31 spp.), Semideciduous Forest (23 spp.), Savanna (9 spp.), and Grasslands (2 spp.). The Serra do Mar and Ribeira Valley regions have the highest concentration of species, as well as the highest index of endemism. The Lauráceas State Park is a strategic conservation unit, because it houses two thirds of the species of Paraná, including seven endemic.
DOI: 10.6084/m9.figshare.9599483
title: Molecular markers indicate the phylogenetic identity of southern Brazilian sea asparagus: first record of Salicornia neei in Brazil

description: Abstract Molecular phylogenetic analyses based on ETS, ITS and atpB - rbcL spacer sequences assessed the phylogenetic status of the southern Brazil sea asparagus species of the genus Salicornia (Salicornioideae, Amaranthaceae). Accessions of Patos Lagoon estuary (32° S) were obtained from wild plants and two pure line lineages, selected from contrasting prostrate (BTH1) and decumbent (BTH2) ecomorphotypes found locally. Patos Lagoon wild plants, BTH1 and BTH2 f4 progenies showed 100% identical sequences for the atpB - rbcL and ITS spacers, only two mutations for ETS. Comparison of the sequences of these three markers with GenBank records confirmed the identity of Brazilian accessions as Salicornia neei . Maximum-likelihood phylogenetic analysis of ETS sequences indicated that the southern Brazilian accessions of Salicornia certainly are not close to any of the Salicornia ambigua accessions in GenBank, which are restricted to the northern hemisphere, nor are they related to any Salicornia fruticosa/Salicornia perennis clade accessions, which are also restricted to Eurasia. All above cited species have been wrongly applied to the southern Brazil sea asparagus.
DOI: 10.6084/m9.figshare.9599483.v1
title: Molecular markers indicate the phylogenetic identity of southern Brazilian sea asparagus: first record of Salicornia neei in Brazil

description: Abstract Molecular phylogenetic analyses based on ETS, ITS and atpB - rbcL spacer sequences assessed the phylogenetic status of the southern Brazil sea asparagus species of the genus Salicornia (Salicornioideae, Amaranthaceae). Accessions of Patos Lagoon estuary (32° S) were obtained from wild plants and two pure line lineages, selected from contrasting prostrate (BTH1) and decumbent (BTH2) ecomorphotypes found locally. Patos Lagoon wild plants, BTH1 and BTH2 f4 progenies showed 100% identical sequences for the atpB - rbcL and ITS spacers, only two mutations for ETS. Comparison of the sequences of these three markers with GenBank records confirmed the identity of Brazilian accessions as Salicornia neei . Maximum-likelihood phylogenetic analysis of ETS sequences indicated that the southern Brazilian accessions of Salicornia certainly are not close to any of the Salicornia ambigua accessions in GenBank, which are restricted to the northern hemisphere, nor are they related to any Salicornia fruticosa/Salicornia perennis clade accessions, which are also restricted to Eurasia. All above cited species have been wrongly applied to the southern Brazil sea asparagus.
DOI: 10.6084/m9.figshare.9599510.v1
title: Zornia (Leguminosae) in the state of Ceará, Northeast Brazil

description: Abstract We present the floristic survey of Zornia occurring in the state of Ceará, as part of the “Flora do Ceará” Project. The study was based on the comparative analysis of morphological characters of specimens in herbaria, samples obtained in field, specialized bibliography, as well as photo-type material. For the State were registered ten species: Zornia afranioi, Z. brasiliensis, Z. cearensis, Z. curvata, Z. guanipensis, Z. harmsiana, Z. latifolia, Z. leptophylla, Z. reticulata and Z. sericea. The species occur mainly in Stepic Savanna (Caatinga), but some were registered in Deciduous Forest (Mata Seca), Savanna (Cerrado), Semi-deciduous Lowland Forest (Mata de Tabuleiro) and Vegetation with Marine Influence (Restinga). Zornia curvata, Z. guanipensis, Z. harmsiana are new occurrences for Ceará state. Three species were recorded in five Conservation Units in state.
DOI: 10.6084/m9.figshare.9599510
title: Zornia (Leguminosae) in the state of Ceará, Northeast Brazil

description: Abstract We present the floristic survey of Zornia occurring in the state of Ceará, as part of the “Flora do Ceará” Project. The study was based on the comparative analysis of morphological characters of specimens in herbaria, samples obtained in field, specialized bibliography, as well as photo-type material. For the State were registered ten species: Zornia afranioi, Z. brasiliensis, Z. cearensis, Z. curvata, Z. guanipensis, Z. harmsiana, Z. latifolia, Z. leptophylla, Z. reticulata and Z. sericea. The species occur mainly in Stepic Savanna (Caatinga), but some were registered in Deciduous Forest (Mata Seca), Savanna (Cerrado), Semi-deciduous Lowland Forest (Mata de Tabuleiro) and Vegetation with Marine Influence (Restinga). Zornia curvata, Z. guanipensis, Z. harmsiana are new occurrences for Ceará state. Three species were recorded in five Conservation Units in state.
DOI: 10.6084/m9.figshare.9599075
title: Edible plants used in Brazil during the 16th and 17th century according to historical reports

description: Abstract This work aimed to study the edible flora in Brazil during the 16th and 17th centuries from analyses of texts of 18 authors who visited the country during this period. The cited plants were identified by similarity through the descriptions and images in the studied texts, area of occurrence, popular name, and the analysis of other botanical studies. In all, 827 citations of food plants were found in the texts studied; it was possible to identify 183 species belonging to 61 different botanical families; 37 citations weren’t possible to identify. Of the plants identified, 55% are native to Americas, including Brazil and other american countries, 8% are endemic to Brazil, 37% are exotic to Americas and for two plants it wasn’t possible to find their origin. From this data it is possible to say that during the 16th and 17th century there was the use of a great cornucopia of plant species by the brazilian population, especially cassava, pineapple, genipap, sweet potato and corn. The presence of exotic and naturalized species native to the Americas shows the exchange of germplasm that already existed among pre-columbian populations. During the 16th and 17th century there was an intense exchange of plants with other regions of the world promoted by Europeans, which resulted in the introduction of many cultivated and exotic species that became important in the brazilian diet. Finally we can say that many of those plants used during that time are now neglected.
DOI: 10.6084/m9.figshare.9599075.v1
title: Edible plants used in Brazil during the 16th and 17th century according to historical reports

description: Abstract This work aimed to study the edible flora in Brazil during the 16th and 17th centuries from analyses of texts of 18 authors who visited the country during this period. The cited plants were identified by similarity through the descriptions and images in the studied texts, area of occurrence, popular name, and the analysis of other botanical studies. In all, 827 citations of food plants were found in the texts studied; it was possible to identify 183 species belonging to 61 different botanical families; 37 citations weren’t possible to identify. Of the plants identified, 55% are native to Americas, including Brazil and other american countries, 8% are endemic to Brazil, 37% are exotic to Americas and for two plants it wasn’t possible to find their origin. From this data it is possible to say that during the 16th and 17th century there was the use of a great cornucopia of plant species by the brazilian population, especially cassava, pineapple, genipap, sweet potato and corn. The presence of exotic and naturalized species native to the Americas shows the exchange of germplasm that already existed among pre-columbian populations. During the 16th and 17th century there was an intense exchange of plants with other regions of the world promoted by Europeans, which resulted in the introduction of many cultivated and exotic species that became important in the brazilian diet. Finally we can say that many of those plants used during that time are now neglected.
DOI: 10.6084/m9.figshare.9599495
title: Cytogenetic studies in three diploid species of Andropogon (Andropogoneae), section Leptopogon

description: Abstract Karyotypes can provide a relevant information about relationships and evolutionary origin among species of the Andropogon genus. This paper presents the karyotype, C+ and DAPI/CMA3 banding and DNA content of three diploid (2n=20) species belonging to section Leptopogon: A. selloanus, A. macrothrix and A. gyrans. Karyotypes of the three diploid species are symmetrical. We propose a karyotype formulae (18m + 2sm) for each of them. The three species show a pair of metacentric chromosomes with a terminal secondary constriction on short arms. Fluorochrome banding revealed different constitutive heterochromatin patterns and CMA3+/DAPI¬ terminal bands related to the nucleolar organizer region in each species. Nuclear DNA content was estimated by flow cytometry ranged from 2.22 to 2.61 pg. FISH technique revealed that these three species have two 45S rDNA loci at the distal ends of the short arms of two metacentric chromosomes. We compare the genomes of the diploids A. selloanus, A. macrothrix and A. gyrans, and the triploid A. ternatus using GISH. These technique allowed us to confirm the hypotheses that the A. selloanus, A. macrothrix and A. gyrans constitute a homogeneous group that share a common S genome that comprises just one of the genomes in the triploid A. ternatus.
DOI: 10.6084/m9.figshare.9599495.v1
title: Cytogenetic studies in three diploid species of Andropogon (Andropogoneae), section Leptopogon

description: Abstract Karyotypes can provide a relevant information about relationships and evolutionary origin among species of the Andropogon genus. This paper presents the karyotype, C+ and DAPI/CMA3 banding and DNA content of three diploid (2n=20) species belonging to section Leptopogon: A. selloanus, A. macrothrix and A. gyrans. Karyotypes of the three diploid species are symmetrical. We propose a karyotype formulae (18m + 2sm) for each of them. The three species show a pair of metacentric chromosomes with a terminal secondary constriction on short arms. Fluorochrome banding revealed different constitutive heterochromatin patterns and CMA3+/DAPI¬ terminal bands related to the nucleolar organizer region in each species. Nuclear DNA content was estimated by flow cytometry ranged from 2.22 to 2.61 pg. FISH technique revealed that these three species have two 45S rDNA loci at the distal ends of the short arms of two metacentric chromosomes. We compare the genomes of the diploids A. selloanus, A. macrothrix and A. gyrans, and the triploid A. ternatus using GISH. These technique allowed us to confirm the hypotheses that the A. selloanus, A. macrothrix and A. gyrans constitute a homogeneous group that share a common S genome that comprises just one of the genomes in the triploid A. ternatus.
DOI: 10.6084/m9.figshare.9957053
title: In vitro propagation of Bucquetia glutinosa, endemic species of the Colombian Paramos

description: Abstract In vitro multiplication protocol of B. glutinosa was established by seeds collected in the Paramo of Rabanal (Boyacá-Colombia), this species is useful in programs for the conservation and restoration of moorland ecosystems. During the establishment of in vitro cultures, 57% of aseptic seeds were obtained with 5 and 10% NaOCl, after 30 days of culture in MS-1, 61.4% of them developing normal appearance seedlings; 75% of the micro-stems cultivated in MS / 2 + 0.5 mg l-1 IBA reached an average length of 2.06 cm, formed up to 10 axillary buds and among these, around two and four basal shoots were individualized. Root development was parallel to multiplication phase. Plants showed a 94.4% survival rate during acclimation in the greenhouse, on a substrate composed of soil and ground mulch (1:1). This is the first micropropagation protocol of native Colombian melastomáceas with a potential use for restoration and conservation of high Andean ecosystems in a state of deterioration and vulnerability.
DOI: 10.6084/m9.figshare.9957053.v1
title: In vitro propagation of Bucquetia glutinosa, endemic species of the Colombian Paramos

description: Abstract In vitro multiplication protocol of B. glutinosa was established by seeds collected in the Paramo of Rabanal (Boyacá-Colombia), this species is useful in programs for the conservation and restoration of moorland ecosystems. During the establishment of in vitro cultures, 57% of aseptic seeds were obtained with 5 and 10% NaOCl, after 30 days of culture in MS-1, 61.4% of them developing normal appearance seedlings; 75% of the micro-stems cultivated in MS / 2 + 0.5 mg l-1 IBA reached an average length of 2.06 cm, formed up to 10 axillary buds and among these, around two and four basal shoots were individualized. Root development was parallel to multiplication phase. Plants showed a 94.4% survival rate during acclimation in the greenhouse, on a substrate composed of soil and ground mulch (1:1). This is the first micropropagation protocol of native Colombian melastomáceas with a potential use for restoration and conservation of high Andean ecosystems in a state of deterioration and vulnerability.
DOI: 10.6084/m9.figshare.9957050
title: New registers of colleters in species of Myrtaceae from Brazilian Cerrado

description: Abstract Colleters are secretory structures that produce a sticky substance, consisting of a mixture of mucilage, proteins, terpenes, pectic substances and even alkaloids, which lubricates and protects the shoot apical meristem. Several colleter types have been described and have taxonomic value in many botanical families. In Myrtaceae, the colleters description is recent and presents three new morphological types (conic, euryform and petaloid) that differ those already described for other eudicots. In this work, we report the colleters morphological types in six species of three genera belonging to the Myrteae tribe of Myrtoideae from the Brazilian Cerrado. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on the fresh and methacrylate-embedded material. The conic and euryform colleters from Myrtoideae species of the Cerrado did not differ either morphologically nor as to the secretion nature from those described for Myrtoideae species from others biomes, which may indicate their potential use for taxonomic purposes. Considering the hypothesis that the multiple fleshy-fruit lineages have evolved independently in Myrteae tribe, our results indicate the relevance of additional studies in order to recognize the pattern of distribution of colleters in Myrtaceae.
DOI: 10.6084/m9.figshare.9957050.v1
title: New registers of colleters in species of Myrtaceae from Brazilian Cerrado

description: Abstract Colleters are secretory structures that produce a sticky substance, consisting of a mixture of mucilage, proteins, terpenes, pectic substances and even alkaloids, which lubricates and protects the shoot apical meristem. Several colleter types have been described and have taxonomic value in many botanical families. In Myrtaceae, the colleters description is recent and presents three new morphological types (conic, euryform and petaloid) that differ those already described for other eudicots. In this work, we report the colleters morphological types in six species of three genera belonging to the Myrteae tribe of Myrtoideae from the Brazilian Cerrado. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on the fresh and methacrylate-embedded material. The conic and euryform colleters from Myrtoideae species of the Cerrado did not differ either morphologically nor as to the secretion nature from those described for Myrtoideae species from others biomes, which may indicate their potential use for taxonomic purposes. Considering the hypothesis that the multiple fleshy-fruit lineages have evolved independently in Myrteae tribe, our results indicate the relevance of additional studies in order to recognize the pattern of distribution of colleters in Myrtaceae.
DOI: 10.6084/m9.figshare.9957026
title: Vascular epiphytes of the restinga forest of Praia do Sul, Ilha Grande, RJ, Brazil

description: Abstract Epiphytic plants show high richness and ecological importance in Atlantic Forest ecosystems. The present study aims to contribute to the knowledge of the vascular epiphyte flora of the restinga forest in Praia do Sul, Ilha Grande, RJ. We demarcated 52 subplots of 100 m2 in the Praia do Sul State Biological Reserve (RBEPS), inspected all the trees and recorded all the epiphytes found. We identified 31 species of vascular epiphytes, 21 genera and nine family, with nine new records for the island. The angiosperms were responsible for 80.6% of the species. Araceae and Philodendron were the family and genus with greatest wealth (22.6% and 16.1%, respectively). The main ecological category in relation to substrate fidelity is holoepiphyte, the form of pollination is entomophilia (64%) and dispersion is zoocoria (58.1%). Most species are endemic to the Atlantic Forest (63%) and have an unknown conservation status (74%). This is the first study on vascular epiphytes in Praia do Sul and highlights the importance of preserving the study area for the conservation of the vascular epiphytes of Ilha Grande and Rio de Janeiro.
DOI: 10.6084/m9.figshare.9957026.v1
title: Vascular epiphytes of the restinga forest of Praia do Sul, Ilha Grande, RJ, Brazil

description: Abstract Epiphytic plants show high richness and ecological importance in Atlantic Forest ecosystems. The present study aims to contribute to the knowledge of the vascular epiphyte flora of the restinga forest in Praia do Sul, Ilha Grande, RJ. We demarcated 52 subplots of 100 m2 in the Praia do Sul State Biological Reserve (RBEPS), inspected all the trees and recorded all the epiphytes found. We identified 31 species of vascular epiphytes, 21 genera and nine family, with nine new records for the island. The angiosperms were responsible for 80.6% of the species. Araceae and Philodendron were the family and genus with greatest wealth (22.6% and 16.1%, respectively). The main ecological category in relation to substrate fidelity is holoepiphyte, the form of pollination is entomophilia (64%) and dispersion is zoocoria (58.1%). Most species are endemic to the Atlantic Forest (63%) and have an unknown conservation status (74%). This is the first study on vascular epiphytes in Praia do Sul and highlights the importance of preserving the study area for the conservation of the vascular epiphytes of Ilha Grande and Rio de Janeiro.
DOI: 10.6084/m9.figshare.9957032.v1
title: Host tree bark traits and development of reintroduced Cattleya intermedia (Orchidaceae) plants in Southern Brazil

description: Abstract The traits of the bark of host trees can influence the distribution and development of epiphytes. Bark samples were suspended in 10 and 20 mL of distilled water for 1, 4 and 24 h, to evaluate pH determination methods. Bark samples of Myrcia brasiliensis, M. glabra and Myrsine coriacea, three host trees of Cattleya intermedia had bark samples collected from the trunk and the crown, for the evaluation of the pH, water retention capacity and thickness. Shoot height (cm) and number of roots of C. intermedia grown on trunk and into the crown of the three species were evaluated. Volume of solvent and suspension time of the bark samples influenced pH values. The pH and thickness of Myrsine coriacea barks were higher when compared to Myrcia species. Shoot height and number of roots of C. intermedia individuals were significantly higher in the crown. The growth parameters of the reintroduced plants were unrelated to pH and bark thickness. The data bring information for the application of further vascular epiphyte studies including new parameters and greater samplings, in order to support proposals of the conservation of endangered species.
DOI: 10.6084/m9.figshare.9957032
title: Host tree bark traits and development of reintroduced Cattleya intermedia (Orchidaceae) plants in Southern Brazil

description: Abstract The traits of the bark of host trees can influence the distribution and development of epiphytes. Bark samples were suspended in 10 and 20 mL of distilled water for 1, 4 and 24 h, to evaluate pH determination methods. Bark samples of Myrcia brasiliensis, M. glabra and Myrsine coriacea, three host trees of Cattleya intermedia had bark samples collected from the trunk and the crown, for the evaluation of the pH, water retention capacity and thickness. Shoot height (cm) and number of roots of C. intermedia grown on trunk and into the crown of the three species were evaluated. Volume of solvent and suspension time of the bark samples influenced pH values. The pH and thickness of Myrsine coriacea barks were higher when compared to Myrcia species. Shoot height and number of roots of C. intermedia individuals were significantly higher in the crown. The growth parameters of the reintroduced plants were unrelated to pH and bark thickness. The data bring information for the application of further vascular epiphyte studies including new parameters and greater samplings, in order to support proposals of the conservation of endangered species.
DOI: 10.6084/m9.figshare.9957047
title: Special offer while stocks last: high-energy pulse of nectar caused by a massive post-fire flowering of Sinningia elatior (Gesneriaceae) in an area of Cerrado and implications for its pollination

description: Abstract The massive post-fire flowering of Sinningia elatior (Gesneriaceae) was examined in an area of Cerrado (Brazilian savanna). To study the reproductive system, pollination tests were conducted in the field. Individuals of S. elatior were found isolated or grouped in the area, the orange to reddish flowers are odorless and tubular. Despite being a protandrous species, S. elatior is self-compatible with spontaneous self-pollination, without apomixis. The male phase lasted two days whereas the female lasted three days. The caloric reward per flower was estimated in 40.8 cal ± 4.2 (n = 20). The massive post-fire flowering of this species provided a large, episodic supply of nectar to nine hummingbirds’ species and 16 insects’ species. While all hummingbirds acted as pollinators, insects were only recorded as nectar/pollen thieves. The S. elatior population offered around 23 Kcal, supplying the energetic needs of approximately two to four hummingbirds per day. Post-fire massive flowering is rare and unpredictable in the Cerrado. Therefore, the fire regime acts shaping the flowering dynamics of some species, producing mosaics of temporary special offers of floral resources that affect not only the foraging dynamics of pollinators but also the crossing patterns of the plant species.