cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.34808/byfv-zw81
title: Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-MAG1L

description: The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and MAG1. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 30-452 of the MAG1 antigen (full length protein). In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.
DOI: 10.34808/yg47-zd34
title: Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-MAG1S

description: The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and MAG1. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 30-222 of the MAG1 antigen (the immunodominant fragment well recognized by antibodies in the early stage of parasite invasion). In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.
DOI: 10.34808/k25z-7k30
title: Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-GRA1

description: The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and GRA1. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 24-190 of the GRA1 antigen. In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.
DOI: 10.34808/gj2c-4m15
title: Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-GRA7

description: The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and GRA7. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 18-236 of the GRA7 antigen. In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.
DOI: 10.34808/mnmb-zx52
title: Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-GRA9

description: The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and GRA9. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 21-318 of the GRA9 antigen. In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.
DOI: 10.25646/8550
title: Tagesdaten-CSV aus dem DIVI-Intensivregister

description: Die Tagesdaten-CSV entspricht einem Auszug der Daten des DIVI-Intensivregisters. Die Datei enthält eine Aggregation der aktuellsten Meldungen pro Landkreis. Es werden die aktuell gemeldeten Anzahlen der COVID-19 Intensivfälle sowie die gemeldeten intensivmedizinischen Behandlungskapazitäten angezeigt. Die Tagesdaten-CSV liefert dabei ausschließlich einen Blick auf die Daten gemäß dem Stand des betrachteten Tages. Die Daten sind im situationsbedingten Kontext aufbereitet, damit sind verschiedene Tagesdaten-CSVs u.U. nicht direkt miteinander vergleichbar. Die aktuellsten Meldungen werden im gewählten Betrachtungszeitfenster über alle Meldebereiche und Standorte aufsummiert. Weitere Informationen sind zu finden unter https://www.intensivregister.de/#/faq
DOI: 10.34740/kaggle/dsv/2279541
title: PIV CounterRotating Turbines
DOI: 10.5523/bris.3rmvxwftl97gi2qxttfi54mjme
title: The ASK study: improving AccesS to Kidney transplantation
title: ASK study interviews

description: Primary study: A living-donor kidney transplant (LDKT) is one of the best treatments for kidney failure. The UK's LDKT activity falls behind that of many other countries, and there is evidence of socioeconomic inequity in access. We aimed to develop a UK-specific multicomponent intervention to support eligible individuals to access a LDKT. The intervention was designed to support those who are socioeconomically-deprived and currently disadvantaged, by targeting mediators of inequity identified in earlier work. We identified three existing interventions in the literature which target these mediators: a) the Norway model (healthcare practitioners contact patients' family with information about kidney donation), b) a home education model, and c) a Transplant candidate advocate model. We undertook intervention development using the Person-Based Approach (PBA). We performed in-depth qualitative interviews with people with advanced kidney disease (n=13), their family members (n=4), and renal and transplant healthcare practitioners (n=15). We investigated participant views on each proposed intervention component. Secondary study: In 2020 England moved to an opt-out deceased donation law. We aimed to investigate the views of relevant stakeholders towards the change in legislation. We investigated the expected impacts of the new legislation on deceased-donor and living-donor transplantation, and participant views on the focus of media campaigns regarding the law change. Available data: 29 of 32 individuals provided consent to data sharing. 29 in-depth qualitative interview transcripts are available. Interviews ranged from 13-74 minutes, with a mean duration of 42 minutes.
DOI: 10.34740/kaggle/dsv/2279572
title: 2021 World Population (updated daily)
DOI: 10.5517/ccdc.csd.cc1n6387
title: CCDC 1525208: Experimental Crystal Structure Determination

description: Related Article: Guoyu Yang, Lijun Shi, Zhenliang Pan, Lulu Wu, Liangxin Fan, Caixia Wang, Cuilian Xu, Jun Liang|2020|Arab.J.Chem.|14|102880|doi:10.1016/j.arabjc.2020.10.027
DOI: 10.5285/df8c4ac0-1723-43ae-ad48-d02d58699f32
title: Thwaites Glacier ice surface elevation change, December 2013 to July 2017, and July 2017 to November 2020

description: Two maps of surface elevation change for Thwaites Glacier, West Antarctica. Change is in metres between 2013-12-21 and 2017-07-11, and between 2017-07-11 and 2020-11-02. The work was funded by NERC projects NE/P011365/1 and NE/S006605/1.
description: The initial digital elevation models (DEMs) were created via an interferometric analysis of TanDEM-X satellite system synthetic aperture radar data. Phase-scaling utilised the supplied orbit data. The DEMs were geocoded first using the RAMP DEM (Liu et al., 2015) and then, iteratively, using the DEMs themselves. The DEMs were calibrated in the vertical using an ICESat-2 point at (107.49°W, 75.47°S) acquired on 2018-11-05. Processing is described fully in the reference paper.
description: Interferometric analysis was carried out using Gamma Remote Sensing software 20190603.
description: Via comparison with ICESat-2 data and in consideration of orbit uncertainty, the original DEMs have an estimated vertical uncertainty of ±3 m, the difference therefore has an estimated uncertainty of ±4.2 m.
DOI: 10.5285/21b3d4fa-0edf-4b05-b762-b4633616b0bc
title: Thwaites Glacier time series of surface elevations at (107.09 W, 75.48 S) from January 2012 to November 2020

description: A time series of surface elevation at a point on Thwaites Glacier, West Antarctica. The point is on grounded ice and is upstream of a sub-shelf cavity on the west flank of the fast-moving core of Thwaites Glacier. There are a total of 88 points. First column = yyyy-mm-dd, second column = elevation in metres. The work was funded by NERC projects NE/P011365/1 and NE/S006605/1.
description: The points were extracted from digital elevation models (DEMs) based on an interferometric analysis of TanDEM-X satellite system synthetic aperture radar (SAR) data. Phase-scaling of the interferograms utilised the supplied orbit data. The DEMs were geocoded first using the RAMP DEM (Liu et al., 2015) and then, iteratively, using the DEMs themselves. The DEMs were calibrated in the vertical using an ICESat-2 point at (107.49°W, 75.47°S) acquired on 2018-11-05. Heights are relative to the WGS84 ellipsoid. Processing is described fully in the reference paper.
description: Feature tracking was carried out using Gamma Remote Sensing software 20190603.
description: 9. Via comparison with ICESat-2 data and in consideration of orbit uncertainty, the original DEMs have an estimated vertical uncertainty of ±3 m.
DOI: 10.5285/ede3520b-cf1c-4979-afcc-94ac266bb61a
title: Thwaites Glacier ice surface elevation profiles from June 2011 to November 2020

description: Thwaites Glacier, West Antarctica. A time series of 156 profiles of ice surface elevation along a flowline based on the mean flow direction. The flowline passes through a region of large elevation change that took place between 2014 and 2017. The work was funded by NERC projects NE/P011365/1 and NE/S006605/1.
description: The profiles were extracted from digital elevation models (DEMs) based on an interferometric analysis of TanDEM-X satellite system synthetic aperture radar (SAR) data. Phase-scaling of the interferograms utilised the supplied orbit data. The DEMs were geocoded first using the RAMP DEM (Liu et al., 2015) and then, iteratively, using the DEMs themselves. The DEMs were calibrated in the vertical using an ICESat-2 point at (107.49°W, 75.47°S) acquired on 2018-11-05. Heights are relative to the WGS84 ellipsoid. Processing is described fully in the reference paper.
description: Interferometric analysis was carried out using Gamma Remote Sensing software 20190603.
description: Via comparison with ICESat-2 data and in consideration of orbit uncertainty, the original DEMs have an estimated vertical uncertainty of ±3 m.
DOI: 10.5285/668bf042-d0de-4741-a62e-2ae93b6f7106
title: Thwaites Glacier ice surface speed change from January 2012 to January 2021

description: A map of changes in ice surface speed in metres/year for Thwaites Glacier, West Antarctica, between January 2012 and January 2021. Speeds based on feature tracking of satellite synthetic aperture radar data. The work was funded by NERC projects NE/P011365/1 and NE/S006605/1.
description: January 2012 speeds were measures using feature-tracking of TerraSAR-X data between a pair of images acquired on 17/01/2012 and 28/01/2012. Speeds were measured at a horizontal resolution of 40 m. Image data were supplied by DLR. The 2021 speeds were measured using feature-tracking Sentinel-1A and Sentinel-1B images and are a mean of all ten 6- or 12-day pairs acquired in January 2021. Speeds were measured at a horizontal resolution of 100 m and oversampled to 40 m before differencing (2021-2012) with the 2012 speeds. Image data were supplied by ESA.
description: Feature-tracking was carried out using Gamma Remote Sensing software 20190603.
description: All feature-tracked displacements were filtered twice: first by a signal to noise ratio based on the cross-correlation of image patches, and then according to deviation from the mean displacement within a neighbourhood. The displacements were converted from the SAR image geometry to ground range geometry, and the results geocoded using the RAMP DEM (Liu et al., 2015). N/A values in the difference geotiff are where either the 2012 or 2021 data are missing.
DOI: 10.5285/c0c1050a-2360-4464-9b0f-c2c101e5d1c2
title: Thwaites Glacier time series ice surface flow speeds at (107.09 W, 75.48 S) from January 2012 to December 2020

description: A time series of surface ice flow speed at a point on Thwaites Glacier, West Antarctica. The point is on grounded ice and is upstream of a sub-shelf cavity on the west flank of the fast-moving core of Thwaites Glacier. There are a total of 589 points. First column = yyyy-mm-dd, second column = speed in kilometres per year. The work was funded by NERC projects NE/P011365/1 and NE/S006605/1.
description: From 2012 to 2014 speeds were measured using feature-tracking of TerraSAR-X image pairs separated by 11 days. TerraSAR-X images were supplied by DLR. Speeds from 2015 onwards were measured using feature-tracking of Sentinel-1A and Sentinel-1B images separated by 6 or 12 days. Sentinel images were supplied by ESA.
description: Feature tracking was carried out using Gamma Remote Sensing software 20190603.
description: All feature-tracked displacements were filtered twice: first by a signal to noise ratio based on the cross-correlation of image patches, and then according to deviation from the mean displacement within a neighbourhood. The displacements were converted from the SAR image geometry to ground range geometry, and the results geocoded using the RAMP DEM (Liu et al., 2015).
DOI: 10.21227/jtdy-xp05
title: Artefacts due to Skin electrode impedance disturbance in multi-frequency bio-impedance measurements

description: This dataset is taken from 20 subjects over a duration of 1 hour where experiments were done on the upper body bio-impedance with the following objectives:a) Evaluate the effect of externally induced perturbance at the SE interface caused by motion, applied pressure, temperature variation and posture change on bio-impedance measurements.b) Evaluate the degree of distortion due to artefact at multiple frequencies (10kHz-100kHz) in the bio-impedance measurements.c) Evaluate the repeatability of induced impedance change due to external perturbance.d) Create a multi-frequency body bio-impedance dataset to develop an algorithm identifying features specific to induced artefacts, and subsequently, to employ artefact suppression.The dataset contains artefact labels for bio-impedance data at ten frequencies, skin-electrode impedance data from four electrodes in tetrapolar configuration and accelerometer data.
DOI: 10.6084/m9.figshare.14695578
title: EEG dataset for evaluation of a single-session SIT effect in healthy aging

description: Thirteen young adults (YA group, mean age 25.5, SD 5.3, 4F/9M) and eleven elderly adults (EA group, mean age 64.2, SD 7.1, 4F/7M) were recruited for the study. All subjects had no medical history of the neural pathological conditions, i.e., stroke, head trauma or tumors.<br><br>Each subject underwent the sensorimotor integration training (SIT) procedure which consisted in <i>N</i> repetitions of elementary sensorimotor integration task. The task required the participants to classify the duration of presented audio stimulus and execute of one of the motor actions (ME) depending on its duration. Specifically, the participant should clench left or right hand into a fist in response to short (SAS) or long (LAS) audio stimulus. The durations of SAS and LAS were 300 ms and 750 ms, respectively. During ME the hand should be held clenched until the next audio stimulus of the same duration, which informed the participant about the end of ME. The time interval required for SI and ME within single trail was chosen randomly in the range 4--5 s. The pause between the trials was also picked randomly within the range 6--8 s. See Fig. 1A for a single trial timeline. The audio stimuli were presented via audio speakers located on the table in front of the participant. During SIT each participant performed <i>N=</i>60 SI task repetitions (30 per stimulus). The overall duration of the experimental session was approximately 10 minutes per participant.<br><br>The raw EEG recordings were filtered using the 50 Hz Notch filter. Additionally, the data were filtered using the 5th-order Butterworth filter in the range 1-100 Hz to remove low-frequency artifacts. The ocular and cardiac artifacts were removed using the independent component analysis (ICA). The filtered time-series were segmented into 60 epochs 6 s long each according to the experiment protocol. Each epoch included 3 s of prestimulus EEG and 3 s of poststimulus EEG centered at the presentation of the first audio stimulus. <br> To evaluate the effect of SIT on cortical activation with trial progression the timeline of experimental session (<i>N=</i>60 epochs total, 30 per stimulus) was divided into four equal Intervals: <i>T1</i> (epochs 1-15); <i>T2</i> (epochs 16-30); <i>T3</i> (epochs 31-45); <i>T4</i> (epochs 46-60). Thus, the interval <i>T1</i> represented early phase, while the interval <i>T4</i> represented the last phase of the experiment. The data was then inspected manually and the epochs with the remaining artifacts were rejected. Finally, each interval contained 10 artifact-free epochs (5 epochs per stimulus), 40 total. See Fig. 1B for sampling epochs into sets.<br>Individual epochs for each subject are stored in the attached ._epo.fif files for <b>Python MNE</b> package for M/EEG analysis. Prefix <b>EA</b> or <b>YA</b> in the name of the file identifies the age group, which subject belongs to. Postfix <b>T1</b>, <b>T2</b>, <b>T3</b> or <b>T4</b> in the name of the file indicates the interval of SIT. <br>
DOI: 10.7479/bbmz-x418
title: Results of Herpetofauna survey from VIETBIO inventory work and field training in the Cuc Phuong National Park (Vietnam)

description: A total of 51 records of 15 amphibians and 17 reptiles were recorded during the surveys in Cuc Phuong National Park. Information about vouchers and other materials such as association, events, taxonomy, geography, media, and dataset for taxa is documented. There are three species including Ingerophrynus galeatus, Ahaetulla cf. prasina, and Raorchestes gryllus are listed in the Vietnam red book (2007) or The IUCN list of Threatened Species (2020).
DOI: 10.6084/m9.figshare.14695790.v1
title: Diamondiferous kimberlites from recently explored Upper Muna field (Siberian craton):petrology, mineralogy and geochemistry insights

description: Table S2. Trace element composition of major minerals
DOI: 10.6084/m9.figshare.14695784.v1
title: Diamondiferous kimberlites from recently explored Upper Muna field (Siberian craton):petrology, mineralogy and geochemistry insights

description: Table S4. Major and trace element composition of kimberlites
DOI: 10.6084/m9.figshare.14695781.v1
title: Diamondiferous kimberlites from recently explored Upper Muna field (Siberian craton):petrology, mineralogy and geochemistry insights

description: Table S1. Major element composition of indicator minerals (concentrates)