DOI: 10.5061/dryad.dbrv15f8d
title: Leaf beetle community data for 20 Iberian localities and associated genetic barcodes (cox1)

description: Field sampling and taxonomic species identification: Leaf beetle
 assemblages were sampled in 20 localities along a South-North transect
 (820 km) in Spain in April–June 2010. Each locality was separated from the
 closest locality by a minimum of 36 km [ALC-UBG] and a maximum of 106 km
 [ANC-EUM]. Sampling localities were selected to maximize the climatic
 variation and spanned an altitudinal range between 120 and 1270 m a.s.l.
 All localities were well preserved areas (most Natural Parks or areas with
 some degree of protection) combining forests dominated by different oak
 species, with shrubs and meadows. Each locality was intensively sampled by
 sweeping and beating all types of vegetation, including trees, shrubs and
 herbs, for 20 sampling periods of 30 min (18 sampling units in UBG).
 Collecting permits were issued by the corresponding regional governments:
 Junta de Andalucía (ALC, UBG, SNS), Junta de Extremadura (JCB, HOR, COR,
 VER, SSP, DEL), Junta de Castilla y León (FRN, ADS, ADN, SAN, OMA, TUE)
 and Xunta de Galicia (LAS, LAR, ANC, MAC, EUM). All specimens were
 preserved in 100% ethanol for DNA extraction. Specimens were identified to
 species level using the taxonomic monographs for the European
 (Warchalowski, 2003) and the Iberian (Petitpierre, 2000) leaf beetle
 faunas. When necessary, male and female genitalia were dissected and
 mounted together with specimens using dimethyl-hydantoin formaldehyde
 resin (DMHF). Sequence data, DNA-based species delimitation and
 phylogenetic analysis: Genomic DNA was extracted from muscle tissue in the
 prothorax region withWizard SV 96-well plates (Promega, UK) or with a
 BioSprint 96 workstation (Qiagen, Germany). A 655 base pair region from
 the 5′ end of mitochondrial cox1 was amplified with primers CO1F2
 (TCTACYAATCATAAAGATATTGGTAC) and CO1R2 (ACTTCTGGATGACCAAAGAATCA) in most
 cases or with standard LCO/HCO primers (Folmer et al., 1994) when the
 previous primers failed. Amplification was performed with Bioline BioTaq
 and the following cycling: 95 °C for 2 min, 35 cycles of 95 °C for 30 s,
 40 °C for 30 s and 72 °C for 45 s, and final extension of 72 °C for 5 min.
 PCR products were cleaned with 96-wellMilliporemultiscreen plates and
 sequenced in both directions using ABI dye terminator sequencing. Sequence
 chromatograms were assembled and manually edited using Genious 5.6. DNA
 sequences are available under GenBank accession numbers KF134544 –
 KF134651 and KF652242 – KF656666.
description: # Leaf beetle community data for 20 Iberian localities and associated
 genetic barcodes (cox1)
 [https://doi.org/10.5061/dryad.dbrv15f8d](https://doi.org/10.5061/dryad.dbrv15f8d) Data used in Baselga et al. 2015 (GEB, doi: 10.1111/geb.12322) and Formoso-Freire et al. 2024 (Ecography, doi: 10.1111/ecog.07200). It includes species abundance data and cox1 5' barcodes for 20 complete leaf beetle communities in Spain. ## Description of the data and file structure Three files are included: specimen_list.csv is a table in which rows are leaf beetle specimens, and columns are sequence identity codes (ID-SEQ), sampling sites (LOC), short species identification code (Sp_id), sequence identity code with species identification (seq_ID), and species name (sp-name). specimen_alignment.fasta is the cox1 5' barcode alignment of the leaf beetle specimens. envdata.csv is a table in which rows are sampling sites, and columns are sampling sites (LOC), geographical coordinates (Longitude and Latitude), altitude (Altitude), and bioclimatic variables for the present and the LGM. Bioclimatic variables are the mean across the sampling points in each locality, and include mean annual temperature in the Last Glacial Maximum (mean_bio1_LGM), annual precipitation in the LGM (mean_bio12_LGM), mean annual temperature in the present (mean_bio1), maximum temperature of the warmest month in the present (mean_bio5), minimum temperature of the coldest month in the present (mean_bio6), annual precipitation in the present (mean_bio12), precipitation of the wettest quarter (mean_bio_16), precipitation of the driest quarter (mean_bio_17), difference in mean annual temperature between present and LGM (temp_diff), and difference in annual precipitation between present and LGM (prec_diff). Geographical coordinates are given in decimal degrees, altitude is given in metres, temperature variables are given in Celsius degrees, and precipitation variables are given in kg/m2.