cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/tz0wv-pp537
title: H. pylori 26695 / AGS

description: Grid box YWC054
description: <strong>Tilt Series Date:</strong> 2013-11-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-22/rawdata/</p>20131125_YWC054_10022.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-22/rawdata/20131125_YWC054_10022.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131125_YWC054_10022_part121_52.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-22/3dimage_35058/20131125_YWC054_10022_part121_52.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.t7pwvz
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Labeo rectipinnis)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008214-250827131500795/datasets/export for details.
DOI: 10.22002/sj3xj-n0960
title: Sulfolobus solfac.P2+STIV - 41KX

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 10 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-02-05</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-05-16/rawdata/</p>atlas8stiv100007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-05-16/rawdata/atlas8stiv100007.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas8stiv100007_part111_26.rec,  Reconstruction (Pixel Size 1.56 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-05-16/3dimage_38446/atlas8stiv100007_part111_26.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t4etk-46m15
title: H. pylori 26695 / AGS

description: Grid box YWC054
description: <strong>Tilt Series Date:</strong> 2013-11-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
  ycw2013-11-25-55_slicer17903.jpg: cag T4SS wi fi particles with pili?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-55/rawdata/</p>20131125_YWC054_10055.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-55/rawdata/20131125_YWC054_10055.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131125_YWC054_10055_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-55/3dimage_35123/20131125_YWC054_10055_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/szd60-1jt27
title: S. pombe (Cdc25-22)

description: Cryosections (180 nm nominal thickness)
description: <strong>Tilt Series Date:</strong> 2014-04-20</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized cytokinesis. From OD 600 = 0.25 grow for 4 hrs at 36 C. Return to 25 C for 45 min then begin monitoring ring formation by LM. Once 80-90 of rings are continuous they were mixed with Dextran (100 ul of 40% Dextran in ~ 400 ul of pelleted cells). They were then high pressure frozen.</p>
<strong>Sample Preparation:</strong> High pressure freezing followed by cryosectioning</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-10/3dimage_42038/</p>042014_pombe_0010.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-10/3dimage_42038/042014_pombe_0010.rec"
        > <i class="download icon"></i> Download </a></p>

        042014_pombe_0010.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-10/rawdata/042014_pombe_0010.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sbw15-0c848
title: H.pylori 26695 WT

description: Grid box YWC089.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-24/rawdata/</p>20140301_YWC089_20016.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-24/rawdata/20140301_YWC089_20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140301_YWC089_20016_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-01-24/3dimage_39450/20140301_YWC089_20016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jn3vz-jt421
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter lysed n the presence of the crowding agent 10% Polyvinylpyrrolidone (PVP)
description: <strong>Tilt Series Date:</strong> 2013-10-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Protocol:-     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xg-     resuspended PVP in 80µL appropriate Buffer 2Buffer 1:Crowding agent1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0Buffer 2:Crowding agentQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-1/rawdata/</p>Rhodopvp27k0002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-1/rawdata/Rhodopvp27k0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp27k0002SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-1/3dimage_46399/Rhodopvp27k0002SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.3j637e
title: Occurrence Download

description: A dataset containing 103 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Cyphotilapia frontosa, Cyprichromis leptosoma, Cyprichromis microlepidotus, Cyrtocara moorii, Dagetichthys commersonnii, Synaptura commersonnii, Dagetichthys lakdoensis, Dalophis boulengeri, Dalophis cephalopeltis, Danakilia franchettii, Datnia elongata, Mesopristes elongatus, Denticeps clupeoides, Dermatopsoides kasougae, Dicentrarchus punctatus, Dicologlossa cuneata, Dimidiochromis compressiceps, Dimidiochromis dimidiatus, Dimidiochromis kiwinge, Dimidiochromis strigatus, Dinotopterus cunningtoni, Diplotaxodon aeneus, Diplotaxodon altus, Diplotaxodon argenteus, Diplotaxodon ecclesi, Diplotaxodon greenwoodi, Diplotaxodon limnothrissa, Diplotaxodon longimaxilla, Diplotaxodon macrops, Distichodus affinis, Distichodus altus, Distichodus antonii, Distichodus atroventralis, Distichodus brevipinnis, Distichodus decemmaculatus, Distichodus engycephalus, Distichodus fasciolatus, Distichodus hypostomatus, Distichodus kolleri, Distichodus langi, Distichodus lusosso, Distichodus maculatus, Distichodus mbiniensis, Distichodus microps, Distichodus mossambicus, Distichodus nefasch, Distichodus noboli, Distichodus notospilus, Distichodus petersii, Distichodus rostratus, Distichodus rufigiensis, Distichodus schenga, Distichodus sexfasciatus, Divandu albimarginatus, Docimodus evelynae, Docimodus johnstoni, Dolichallabes microphthalmus, Dolichamphilius brieni, Dolichamphilius longiceps, Dormitator lebretonis, Doumea angolensis, Doumea chappuisi, Doumea gracila, Doumea sanaga, Doumea thysi, Doumea typica, Drombus bontii, Gobius bontii, Dundocharax bidentatus, Ebomegobius goodi, Eclectochromis ornatus, Ectodus descampsii, Eleotris acanthopoma, Eleotris daganensis, Eleotris fusca, Eleotris mauritiana, Eleotris pellegrini, Eleotris senegalensis, Eleotris vittata, Eleotris vomerodentata, Ellochelon vaigiensis, Elops lacerta, Elops senegalensis, Engraulicypris bredoi, Engraulicypris brevianalis, Engraulicypris gariepinus, Engraulicypris moeruensis, Engraulicypris ngalala, Engraulicypris sardella, Engraulicypris spinifer, Enneacampus ansorgii, Enneacampus kaupi, Enteromius ablabes, Enteromius aboinensis, Enteromius afrohamiltoni, Enteromius afrovernayi, Enteromius aliciae, Enteromius aloyi, Enteromius amanpoae, Enteromius amatolicus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((27.3628 -33.9401,28.1382 -33.4828,28.1382 -24.9401,19.1382 -24.9401,19.1382 -33.9401,27.3628 -33.9401))"
 ]
}

 The dataset includes 103 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008216-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/76sew-37181
title: Bdellovibrio/Vibrio cholerae

description: Bdellovibrio invasion complex with Vibrio cholerae MKW1383.Imaged by Janelia farm Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-05-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-12/rawdata/</p>grid3-tiltseries0012.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-12/rawdata/grid3-tiltseries0012.mrc"
        > <i class="download icon"></i> Download </a></p>

        grid3-tiltseries0012_part131_2.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-12/3dimage_26407/grid3-tiltseries0012_part131_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7eqjf9
title: Occurrence Download

description: A dataset containing 9 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lagarosiphon major",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 9 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008215-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/xjdjh-rhf06
title: Myxococcus xanthus delta-pilB

description: grid box YWC066
description: <strong>Tilt Series Date:</strong> 2013-12-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2013-12-31-23_slicer8995.jpg: filament bundle .  Myxococcus xanthus ycw2013-12-31-23_slicer8998.jpg: filament .  Myxococcus xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-31-23/rawdata/</p>20131231_YWC066_10023.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-31-23/rawdata/20131231_YWC066_10023.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131231_YWC066_10023_part131_4.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-31-23/3dimage_36973/20131231_YWC066_10023_part131_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qk8sb-g2n07
title: Helicobacter pylori 26695

description: Cells are sick. Don't have flagella. Only see partial motors.
description: <strong>Tilt Series Date:</strong> 2013-10-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in brain heart infusion broth + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-10-5_slicer10183.jpg: bunch of small vesicles, Helicobacter pylori
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-10-5/rawdata/</p>20131010_Hpylori_26695_BHI_20005.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-10-5/rawdata/20131010_Hpylori_26695_BHI_20005.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131010_Hpylori_26695_BHI_20005_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
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DOI: 10.15468/dl.btn2sq
title: Occurrence Download

description: A dataset containing 821 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lagarosiphon major",
 "Country is Belgium",
 "HasCoordinate is true"
 ]
}

 The dataset includes 821 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008218-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.