cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4580845.v1
title: Single-stage versus two-stage models to estimate creatinine corrected urinary analyte concentrations

description: The ability to detect 'known' differences in urinary analyte concentrations due to gender, age, and race/ethnicity when adjusted for similar differences in urinary creatinine concentrations were evaluated by a single-stage and a two-stage model by ten simulation studies. Log10 transformed values of observed urinary analyte concentration were used as the dependent variable and age, gender, and race/ethnicity were used as the categorical independent variables. In addition, while single-stage model used log10 transformed values of urinary creatinine as a covariate, two-stage model used a correction factor (CF) determined during the first stage of the model by fitting a secondary model for urinary creatinine. Single-stage model was almost always able to statistically significantly detect 'known' differences due to age, gender, and race/ethnicity. On the other hand, two-stage model was able to statistically significantly detect 'known' differences due to age, gender, and race/ethnicity a maximum of 87.2% of the times and as low as 10.6% of the times primarily because of the presence of multicollinearity between CF and urinary creatinine concentrations. Consequently, as long as the sole objective is to estimate the urinary analyte concentrations adjusted for the effect of all factors including urinary creatinine, single-stage models are the models of choice.
DOI: 10.6084/m9.figshare.4580887.v1
title: Supplementary Material for: Methotrexate Induces Apoptosis of Postpartum Placental Cytotrophoblasts

description: <b><i>Background:</i></b> Though methotrexate (MTX) is known to inhibit proliferation of trophoblasts derived from ectopic and intrauterine pregnancies, its action on trophoblasts derived from postpartum placenta remains questionable. This study was designed to ascertain the efficacy of MTX in inducing cell death of postpartum placental cytotrophoblasts (PPTC). <b><i>Methodology:</i></b> Primary human cytotrophoblasts were isolated from placentae of 1st and 2nd trimester intrauterine pregnancies and from postpartum placentae. The isolated trophoblasts were identified based on the expression of cytokeratin 7. MTX-induced inhibition of proliferation of cytotrophoblasts was detected by flow cytometry combined with the WST-1 assay. Secretion of HCG-β and invasiveness were evaluated to assess the effect of MTX on blocking the differentiated cellular function of cytotrophoblasts in relation to the gestational age. The efficacy of MTX in inducing apoptosis of cytotrophoblasts was estimated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. <b><i>Results:</i></b> MTX significantly inhibited the proliferation and differentiation of cytotrophoblasts. MTX-induced cell apoptosis of PPTC was confirmed by increased expression of Fas, FasL, Bax, cleaved caspases 3, 7, 8, and 9, and decreased expression of Bcl-2. <b><i>Conclusion:</i></b> MTX inhibits replication and differentiation of cytotrophoblasts and appears to be an efficient inducer of PPTC apoptosis.
DOI: 10.6084/m9.figshare.4580890.v1
title: Supplementary Material for: Association of Albuminuria and Estimated Glomerular Filtration Rate with Functional Performance Measures in Older Adults with Chronic Kidney Disease

description: <b><i>Background:</i></b> Chronic kidney disease (CKD) is increasingly common and disproportionately affects older adults. The contribution of kidney disease to the functional impairment noted in the elderly CKD population is unclear. <b><i>Methods:</i></b> This is a cross-sectional analysis of a hypertensive cohort of people aged ≥75 years from the Systolic Blood Pressure Intervention Trial. We evaluated estimated glomerular filtration rate (eGFR) and urine albumin-to-creatinine ratio (UACR) as predictors of 3 measures of functional status: EuroQol-5 Dimensional (EQ-5D) score, Falls Efficacy Scale (FES) score, and gait speed. Linear regression models were used to evaluate the associations between our independent variables and outcome measures. <b><i>Results:</i></b> Our analysis included 2,620 participants, mean age of 79.9 (4.0) years. Unadjusted models showed that lower eGFR level and higher UACR level were associated with lower EQ-5D (<i>p</i> &lt; 0.001 for both) and slower gait speed (<i>p</i> &lt; 0.001 for both) and worse scores on FES (<i>p</i> = 0.032 and <i>p</i> = 0.039). In the fully adjusted models, higher levels of UACR remained significantly associated with lower EQ-5D scores and slower gait speed (<i>p</i> = 0.011 and <i>p</i> = 0.002, respectively). In contrast, level of eGFR was not associated with any functional outcome measures when accounting for covariates. <b><i>Conclusions:</i></b> In individuals aged ≥75 years, albuminuria and eGFR were associated with impairments in physical performance and self-reported functional status; however, only the association with albuminuria remained after adjusting for relevant demographics and comorbidities. Evaluation of albuminuria may provide an additional tool for identifying older individuals at risk for functional impairment.
DOI: 10.6084/m9.figshare.4580800
title: A new genus of schizomids (Arachnida: Schizomida: Hubbardiidae) from Mexico, with notes on its systematics

description: During the systematic revision of <i>Mayazomus</i>, the third most diverse genus of micro whip-scorpions in North America, we found three species with peculiar morphological variation, which are different from those diagnostic characters for <i>Mayazomus</i>. These species share with <i>Mayazomus</i> the large body size and the unusual development of the male pedipalps; however, these three species share the shape of the female spermathecae with <i>Stenochrus</i> rather than with <i>Mayazomus.</i> Therefore, their phylogenetic placements create a dilemma. Here we perform analyses with morphological evidence, using two different methodologies (Parsimony and Bayesian inference) in order to hypothesize the phylogenetic relationships of the three species mentioned with each other and with respect to other North American and/or Central American genera. The results confirm our initial hypothesis that the species belong to a different genus, <i>Olmeca</i> gen. nov., which is recovered as the sister group of all hubbardiine genera included in these analyses, except for the genus <i>Hubbardia</i>. The results also indicate that despite sharing some morphological similarities, it is distantly related with <i>Mayazomus</i>. Three species are described herein: <i>Olmeca brujo</i> sp. nov., <i>Olmeca cruzlopezi</i> sp. nov., and <i>Olmeca santibanezi</i> sp. nov. Additionally, we provide a hypothesis of the relationships and biogeography of <i>Olmeca</i> gen. nov. with other genera. http://zoobank.org/urn:lsid:zoobank.org:pub:F64CE801-7D82-4827-A5B9-0C0B41F58043
DOI: 10.6084/m9.figshare.4580773
title: <i>Heterostegina matteuccii</i> sp. nov. (Foraminiferida: Nummulitidae) from the lower Oligocene of Sicily and Aquitaine: a possible transatlantic immigrant

description: The systematics and phylogenetic relations of the Oligocene species of <i>Heterostegina</i> (Nummulitidae) are poorly known compared to those of Eocene and Miocene species. The new species <i>Heterostegina matteuccii</i> sp. nov., characterized by the combination of an advanced nepionic stage (i.e. very few operculine chambers) and a relatively small proloculus, is here described from the lower Oligocene Caltavuturo Formation of Portella Colla (Madonie Mts, Sicily, Italy). The same species is recorded also in the ‘Calcaire à astéries’ Formation of Illats (Northern Aquitaine, near Bordeaux, France). Biometrically, <i>H. matteuccii</i> sp. nov. does not fit within any known Neo-Tethyan lineage of <i>Heterostegina</i> from the Eocene to the Miocene, and hence is considered to be a member of a distinct phyletic lineage. The advanced nepionic stage of <i>H</i>. <i>matteuccii</i> sp. nov., however, calls for the existence of predecessors. The Priabonian (and possibly also earliest Oligocene) <i>H</i>. <i>ocalana</i> Cushman, 1921 from the American-Caribbean realm is here interpreted as the most probable ancestral form for the <i>H</i>. <i>matteuccii</i> lineage. Thus, an early Oligocene eastward transatlantic migration of this lineage may be inferred. New results of strontium isotope stratigraphy from both sides of the Atlantic Ocean are consistent with this hypothesis. http://zoobank.org/urn:lsid:zoobank.org:pub:CC54DB64-091D-4CDE-A6AB-6DC620F0FC23
DOI: 10.6084/m9.figshare.4580800.v1
title: A new genus of schizomids (Arachnida: Schizomida: Hubbardiidae) from Mexico, with notes on its systematics

description: During the systematic revision of <i>Mayazomus</i>, the third most diverse genus of micro whip-scorpions in North America, we found three species with peculiar morphological variation, which are different from those diagnostic characters for <i>Mayazomus</i>. These species share with <i>Mayazomus</i> the large body size and the unusual development of the male pedipalps; however, these three species share the shape of the female spermathecae with <i>Stenochrus</i> rather than with <i>Mayazomus.</i> Therefore, their phylogenetic placements create a dilemma. Here we perform analyses with morphological evidence, using two different methodologies (Parsimony and Bayesian inference) in order to hypothesize the phylogenetic relationships of the three species mentioned with each other and with respect to other North American and/or Central American genera. The results confirm our initial hypothesis that the species belong to a different genus, <i>Olmeca</i> gen. nov., which is recovered as the sister group of all hubbardiine genera included in these analyses, except for the genus <i>Hubbardia</i>. The results also indicate that despite sharing some morphological similarities, it is distantly related with <i>Mayazomus</i>. Three species are described herein: <i>Olmeca brujo</i> sp. nov., <i>Olmeca cruzlopezi</i> sp. nov., and <i>Olmeca santibanezi</i> sp. nov. Additionally, we provide a hypothesis of the relationships and biogeography of <i>Olmeca</i> gen. nov. with other genera. http://zoobank.org/urn:lsid:zoobank.org:pub:F64CE801-7D82-4827-A5B9-0C0B41F58043
DOI: 10.6084/m9.figshare.4580773.v1
title: <i>Heterostegina matteuccii</i> sp. nov. (Foraminiferida: Nummulitidae) from the lower Oligocene of Sicily and Aquitaine: a possible transatlantic immigrant

description: The systematics and phylogenetic relations of the Oligocene species of <i>Heterostegina</i> (Nummulitidae) are poorly known compared to those of Eocene and Miocene species. The new species <i>Heterostegina matteuccii</i> sp. nov., characterized by the combination of an advanced nepionic stage (i.e. very few operculine chambers) and a relatively small proloculus, is here described from the lower Oligocene Caltavuturo Formation of Portella Colla (Madonie Mts, Sicily, Italy). The same species is recorded also in the ‘Calcaire à astéries’ Formation of Illats (Northern Aquitaine, near Bordeaux, France). Biometrically, <i>H. matteuccii</i> sp. nov. does not fit within any known Neo-Tethyan lineage of <i>Heterostegina</i> from the Eocene to the Miocene, and hence is considered to be a member of a distinct phyletic lineage. The advanced nepionic stage of <i>H</i>. <i>matteuccii</i> sp. nov., however, calls for the existence of predecessors. The Priabonian (and possibly also earliest Oligocene) <i>H</i>. <i>ocalana</i> Cushman, 1921 from the American-Caribbean realm is here interpreted as the most probable ancestral form for the <i>H</i>. <i>matteuccii</i> lineage. Thus, an early Oligocene eastward transatlantic migration of this lineage may be inferred. New results of strontium isotope stratigraphy from both sides of the Atlantic Ocean are consistent with this hypothesis. http://zoobank.org/urn:lsid:zoobank.org:pub:CC54DB64-091D-4CDE-A6AB-6DC620F0FC23
DOI: 10.6084/m9.figshare.4580812.v2
title: ODMeasurements of MIC.xlsx

description: The MIC values were determined using a 96-well Microtiter plate assay. Antibiotics were prepared after sugested concentrations of J.M. Andrews (2001). The plate layout used for the MIC tests were all the same. Bacteriocins in row A-D and antibiotics in row E-H, The test solutions were undiluted in column 1 and two-fold dilutions were made from column 2-10. Column 11 and 12 were controls, a growth control with inoculated Müller-Hinton broth (MHB) and a sterility control with MHB. The pathogens were taken from glycerol stocks and were incubated in MHB overnight, except S. pneumoniae which were incubated for 6h. After incubation OD450 were measured and the cultures were diluted in an appropriate volume of MHB to a OD450 = 0, 01. Each well in column 1-11 were inoculated with the same pathogen and covered with film. Each plate setup was done in triplets and incubated at 37oC for 16h. After incubation OD450 were measured for further analysis.
DOI: 10.6084/m9.figshare.4580770
title: Leaves of <i>Podozamites</i> and <i>Pseudotorellia</i> from the Early Cretaceous of Mongolia: stomatal patterns and implications for relationships

description: Strap-shaped, parallel-veined leaves of <i>Podozamites</i> and <i>Pseudotorellia</i> are among the most common fossils in Late Triassic to Early Cretaceous floras from the Northern Hemisphere. <i>Podozamites</i> is considered to be the leaf of a voltzian conifer, but its cuticle is poorly known, whereas <i>Pseudotorellia</i>, with a thick cuticle and haplocheilic stomata, is commonly considered to be the leaf of a ginkgoalean. Here we describe the leaf morphology, cuticle and stomata of <i>Podozamites</i> and <i>Pseudotorellia</i> based on excellently preserved material from the Early Cretaceous of central Mongolia. <i>Podozamites harrisii</i> sp. nov. has transversely oriented, paracytic (probably syndetocheilic) stomata that are regularly arranged in longitudinal files. <i>Pseudotorellia resinosa</i> sp. nov. and <i>Pseudotorellia palustris</i> sp. nov. have scattered, longitudinally oriented stomata in which the two guard cells are sunken and surrounded by 2–5 specialized lateral subsidiary cells and 1–3 unspecialized polar cells. Association evidence and similarities in cuticular structure suggest that <i>Podozamites harrisii</i> was produced by the same plant as the seed cone <i>Krassilovia</i>. The distinctive stomatal pattern of <i>Podozamites harrisii</i> and <i>Krassilovia</i> is also seen in some species of <i>Swedenborgia</i> and <i>Cycadocarpidium</i>, suggesting these plants may all belong to the same natural group. <i>Cycadocarpidium</i>, <i>Krassilovia</i> and <i>Swedenborgia</i> have previously been treated as conifers, but their transversely oriented, paracytic stomata hint instead at a possible relationship with Bennettitales and Gnetales.
DOI: 10.6084/m9.figshare.4580770.v1
title: Leaves of <i>Podozamites</i> and <i>Pseudotorellia</i> from the Early Cretaceous of Mongolia: stomatal patterns and implications for relationships

description: Strap-shaped, parallel-veined leaves of <i>Podozamites</i> and <i>Pseudotorellia</i> are among the most common fossils in Late Triassic to Early Cretaceous floras from the Northern Hemisphere. <i>Podozamites</i> is considered to be the leaf of a voltzian conifer, but its cuticle is poorly known, whereas <i>Pseudotorellia</i>, with a thick cuticle and haplocheilic stomata, is commonly considered to be the leaf of a ginkgoalean. Here we describe the leaf morphology, cuticle and stomata of <i>Podozamites</i> and <i>Pseudotorellia</i> based on excellently preserved material from the Early Cretaceous of central Mongolia. <i>Podozamites harrisii</i> sp. nov. has transversely oriented, paracytic (probably syndetocheilic) stomata that are regularly arranged in longitudinal files. <i>Pseudotorellia resinosa</i> sp. nov. and <i>Pseudotorellia palustris</i> sp. nov. have scattered, longitudinally oriented stomata in which the two guard cells are sunken and surrounded by 2–5 specialized lateral subsidiary cells and 1–3 unspecialized polar cells. Association evidence and similarities in cuticular structure suggest that <i>Podozamites harrisii</i> was produced by the same plant as the seed cone <i>Krassilovia</i>. The distinctive stomatal pattern of <i>Podozamites harrisii</i> and <i>Krassilovia</i> is also seen in some species of <i>Swedenborgia</i> and <i>Cycadocarpidium</i>, suggesting these plants may all belong to the same natural group. <i>Cycadocarpidium</i>, <i>Krassilovia</i> and <i>Swedenborgia</i> have previously been treated as conifers, but their transversely oriented, paracytic stomata hint instead at a possible relationship with Bennettitales and Gnetales.
DOI: 10.6084/m9.figshare.4580812.v1
title: OD.xlsx

description: The MIC values were determined using a 96-well Microtiter plate assay. Antibiotics were prepared after sugested concentrations of J.M. Andrews (2001). The plate layout used for the MIC tests were all the same. Bacteriocins in row A-D and antibiotics in row E-H, The test solutions were undiluted in column 1 and two-fold dilutions were made from column 2-10. Column 11 and 12 were controls, a growth control with inoculated Müller-Hinton broth (MHB) and a sterility control with MHB. The pathogens were taken from glycerol stocks and were incubated in MHB overnight, except S. pneumoniae which were incubated for 6h. After incubation OD450 were measured and the cultures were diluted in an appropriate volume of MHB to a OD450 = 0, 01. Each well in column 1-11 were inoculated with the same pathogen and covered with film. Each plate setup was done in triplets and incubated at 37oC for 16h. After incubation OD450 were measured for further analysis.
DOI: 10.6084/m9.figshare.4580755
title: Glacial geomorphology of the northern Kivalliq region, Nunavut, Canada, with an emphasis on meltwater drainage systems

description: This paper presents a glacial geomorphological map of glacial lineations, ribbed terrain, moraines, meltwater channels (subglacial and ice-marginal/proglacial), eskers, glaciofluvial deposits, ice-contact outwash fans and deltas and abandoned shorelines on the bed of the former Laurentide Ice Sheet in northern Canada. Mapping was compiled from satellite imagery and digital elevation data and landforms were digitised directly into a Geographical Information System. The map reveals a complex glacial history characterised by multiple ice-flow events, including fast-flowing ice streams. Moraines record a series of pauses or re-advances during overall SE retreat towards the Keewatin Ice Divide. The distribution of subglacial meltwater landforms indicates that several distinctive scales and modes of drainage system operated beneath the retreating ice sheet. This includes a large (&gt;100 km) integrated network of meltwater channels, eskers, ice-contact outwash fans and deltas and glaciofluvial deposits that originates at the northern edge of Aberdeen Lake. The map comprises zone 66 of the Canadian National Topographic System, which encompasses an area of 160,000 km<sup>2</sup>. It is presented at a scale of 1:500,000 and is designed to be printed at A0 size.
DOI: 10.6084/m9.figshare.4580752
title: Digestive cell lysosomes as main targets for Ag accumulation and toxicity in marine mussels, <i>Mytilus galloprovincialis</i>, exposed to maltose-stabilised Ag nanoparticles of different sizes

description: Bioavailability and toxicity of maltose-stabilised AgNPs of different sizes (20, 40 and 100 nm) in mussels were compared with bulk and aqueous forms of the metal through a two-tier experimental approach. In the first tier, mussels were exposed for 3 d to a range of concentrations (0.75, 75, 750 μg Ag/l) in the form of Ag20-Mal, Ag40-Mal, Ag100-Mal, bulk Ag and aqueous Ag (as AgNO<sub>3</sub>), as well as to the concentrations of maltose used in the formulation of NPs. Mortality, bioaccumulation, tissue and cell distribution and lysosomal responses were investigated. In the second tier, mussels were exposed for 21 d to Ag20-Mal, Ag100-Mal, bulk Ag and aqueous Ag at the lowest effective concentration selected after <i>Tier 1</i> (0.75 μg Ag/l), biomarkers and toxicopathic effects were investigated. Aqueous Ag was lethal within 3 d at 75 μg Ag/l; Ag NPs or bulk Ag did not produce significant mortality at 750 μg Ag/l. Ag accumulation was limited and metallothionein gene transcription was not regulated although metal accumulation occurred in digestive, brown and stomach epithelial cells and in gut lumen after exposure to AgNPs and aqueous Ag starting at low concentrations after 1 d. Electrondense particles (&lt;10 nm) in lysosomes and residual bodies after exposure to AgNPs contained Ag and S (X-ray). Intralysosomal metal accumulation and lysosomal membrane destabilisation were enhanced after exposure to all the forms of Ag and more marked after exposure to Ag20-Mal than to larger NPs. 21 d exposure to AgNPs provoked digestive cell loss and loss of digestive gland integrity, resulting in atrophy-necrosis in digestive alveoli and oedema/hyperplasia in gills (Ag NP), vacuolisation in digestive cells (aqueous Ag) and haemocyte infiltration of connective tissue (all treatments). Intralysosomal metal accumulation, lysosomal responses and toxicopathic effects are enhanced at decreasing sizes and appear to be caused by Ag<sup>+ </sup>ions released from NPs, although the metal was not substantially accumulated.
DOI: 10.6084/m9.figshare.4580755.v1
title: Glacial geomorphology of the northern Kivalliq region, Nunavut, Canada, with an emphasis on meltwater drainage systems

description: This paper presents a glacial geomorphological map of glacial lineations, ribbed terrain, moraines, meltwater channels (subglacial and ice-marginal/proglacial), eskers, glaciofluvial deposits, ice-contact outwash fans and deltas and abandoned shorelines on the bed of the former Laurentide Ice Sheet in northern Canada. Mapping was compiled from satellite imagery and digital elevation data and landforms were digitised directly into a Geographical Information System. The map reveals a complex glacial history characterised by multiple ice-flow events, including fast-flowing ice streams. Moraines record a series of pauses or re-advances during overall SE retreat towards the Keewatin Ice Divide. The distribution of subglacial meltwater landforms indicates that several distinctive scales and modes of drainage system operated beneath the retreating ice sheet. This includes a large (&gt;100 km) integrated network of meltwater channels, eskers, ice-contact outwash fans and deltas and glaciofluvial deposits that originates at the northern edge of Aberdeen Lake. The map comprises zone 66 of the Canadian National Topographic System, which encompasses an area of 160,000 km<sup>2</sup>. It is presented at a scale of 1:500,000 and is designed to be printed at A0 size.
DOI: 10.6084/m9.figshare.4580701
title: <sup>40</sup>Ar/<sup>39</sup>Ar ages. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S4: Summary of Early Mesozoic metamorphic, deformation and granite-related Nb–Ta–W–Sn.
DOI: 10.6084/m9.figshare.4580704
title: Major and trace element data. Early Mesozoic intracontinental orogeny and stress transmission in South China: evidence from Triassic peraluminous granites

description: Table S3: Major REEs Trace of 15 samples.