DOI: 10.5061/dryad.7m0cfxq3k
title: Data for: Evidence to support phytosanitary policies - The minimum effective heat treatment parameters for pathogens associated with forest products

description: Research on reducing the movement of pests on wood products has led
 to several options for safer trade including heat treatment of
 wood to mitigate pests. In this study, pathogenic organisms
 commonly regulated in the trade of forest products were tested to
 determine the minimum heat dose (temperature and time) required
 to cause mortality. The mycelial stage of tree
 pathogens, Heterobasidion occidentale, Grosmannia clavigera,
 Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum
 and P. xmultiformis, which may be found in wood products, were tested in
 vitro using the Humble water bath with parameters simulating the rate of
 heat applied to wood in a commercial kiln. The lethal temperature for the
 pathogens ranged from 44 to 50°C for a 30-minute treatment duration. A
 molecular diagnostic method to confirm pathogen mortality was available
 for five of the species heat treated. RNA detection using reverse
 transcription real-time PCR was used to validate pathogen mortality
 following treatment for: P. ramorum, P. lateralis, P. cinnamomi, P.
 xmultiformis and G. clavigera. The heat treatment data set is
 deposited here.
description: The data are observations on post-treatment survival of
 <em>Heterobasidion occidentale</em>, <em>Grosmannia
 clavigera</em>, <em>Bretziella fagacearum</em>,
 <em>Phytophthora cinnamomi</em>, <em>P.
 lateralis</em>, <em>P. ramorum</em> and
 <em>P</em>. x<em>multiformis</em> exposed to heat
 in a water bath. The experiments were used to determine the optimal
 heating conditions for treatment of wood infested with forest pathogens.
 The mycelial stage of pathogens growing on grain were exposed to
 temperatures from 40 up to 52 degrees celsius for 30 minutes. Heat-treated
 and unexposed control grains with mycelium were plated on 20% V8 agar for
 <em>Phytophthora</em> species or 2% malt extract agar with
 yeast extract for fungi and observed for growth to determine if they
 survived treatment at each of the exposure temperatures.
description: # Data for: Evidence to support phytosanitary policies -The minimum
 effective heat treatment parameters for pathogens associated with forest
 products
 [https://doi.org/10.5061/dryad.7m0cfxq3k](https://doi.org/10.5061/dryad.7m0cfxq3k) Data are observations on post-treatment survival of *Heterobasidion occidentale*, *Grosmannia clavigera*, *Bretziella fagacearum*, *Phytophthora cinnamomi*, *P. lateralis*, *P. ramorum* and *P. xmultiformis* exposed to heat in a water bath at temperatures ranging from 40 up to 52 degrees Celsius for 30 minutes. # Description of the data and file structure These data were assembled and summarized from raw data sheets and transferred into excel format. Description of fields: * Experiment number: The experimental run within which the data were collected  * Experiment date: The date on which the experimental run was conducted * Species: The Latin name of the pathogen containing the first initial of the genus followed by the species name * Isolate: The unique identifier for each specimen corresponding to the identity assigned by the holder of the culture collection from which the sample was received. * Temperature_C: The temperature in degrees Celsius that each specimen was tested. * Duration_Min: The treatment duration in minutes measured from when the last probe in the water bath reached the target treatment temperature * Replicate: The replicate test of each isolate growing on independent grains, treated in the same run, under the same conditions. * Survival: post treatment outcome, either Y or N, where Y is survival measured by observed mycelial growth and N is no growth observed indicating no survival. Missing data is indicated by a blank. ## Sharing/Access information There are no other available versions of the data. ## Code/Software N/A