DOI: 10.58132/p6wdss
title: Dynamics of PsaA and Representative Plastid Proteins in Selected Angiosperm Species During Etiolation and Greening

description: This dataset contains results from experiments on plastid protein composition, plastid complexes, and gene expression comparing several plant species (Arabidopsis thaliana, Phaseolus coccineus, Pisum sativum, Avena sativa) during a 10-day etiolation process at selected time points and under reference greening and illumination conditions.File name: species_western_blot.zip – compressed archive containing Western blot scans (.tif, .png) of etioplast and chloroplast proteins (PsaA, PsaB, Alb3, Ycf4, Lhca2) in samples of Arabidopsis thaliana, Phaseolus coccineus, Pisum sativum, and Avena sativa collected after 10 days of etiolation – 0d, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Additional Coomassie Briliant Blue R-250 (CBB)-stained gels represent total protein loading controls. The archive includes a README.txt file with lane annotations and specifications. File naming convention: Species_Protein_ReplicateNumber.File name: Avena_western_blot.zip – compressed archive containing Western blot scans (.tif, .png) of etioplast and chloroplast proteins (PsaA, PsaB, PsaC, PsaD, Lhca2) in samples of Avena sativa collected during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation), 3 hours of light illumination after 10 days od etiolation – 0d3hL, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Additional Coomassie Briliant Blue R-250 (CBB)-stained gels represent total protein loading controls. The archive includes a README.txt file with lane annotations and specifications. File naming convention: Species_Protein_ReplicateNumber. Sample order on the membranes: Avena_sativa_-10d, Avena_sativa_-7d, Avena_sativa_-5d, Avena_sativa_-3d, Avena_sativa_0d, Avena_sativa_0d3hL, Avena_sativa_20dL/3, Avena_sativa_20dL.File name: Pisum_western_blot.zip– compressed archive containing Western blot scans (.tif, .png) of etioplast and chloroplast proteins (PsaA, PsaB, PsaC, PsaD, Lhca2) in samples of Pisum sativum collected during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation), 3 hours of light illumination after 10 days of etiolation – 0d3hL, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Additional Coomassie Briliant Blue R-250 (CBB)-stained gels represent total protein loading controls. The archive includes a README.txt file with lane annotations and specifications. File naming convention: Species_Protein_ReplicateNumber. Sample order on the membranes: Pisum_sativum_-10d, Pisum_sativum_-7d, Pisum_sativum_-5d, Pisum_sativum_-3d, Pisum_sativum_0d, Pisum_sativum_0d3hL, Pisum_sativum_20dL/3, Pisum_sativum_20dL.File name: species_BN.zip – compressed archive containing Western blot scans (.tif, .png) of etioplast and chloroplast proteins within native protein complexes (PsaA) in samples of Pisum sativum and Avena sativa collected after 10 days of etiolation – 0d, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Additional Blue Native PAGE gels represent total protein complexes resolving controls. The archive includes a README.txt file with lane annotations and specifications. File naming convention: Species_Protein_ BN_ReplicateNumber. Sample order on the membranes: Pisum_sativum_0d, Avena_sativa_0d, Pisum_sativum_20dL, Avena_sativa_20dL.File name: Pisum_BN_western_blot.zip – compressed archive containing Western blot scans (.tif, .png) of etioplast proteins within native protein complexes (PsaA, PsaB, FNR, POR, Chlorophyll synthase) in samples of Pisum sativum collected after 10 days of etiolation – 0d. Additional Blue Native PAGE gels represent total protein complexes resolving controls. The archive includes a README.txt file with lane annotations and specifications. File naming convention: Species_Protein_ BN_ReplicateNumber.File name: species_RT-qPCR.zip – . archive containing raw RT-qPCR (.txt format) results for plastid genes (psaA, psaB, atpB, Lhca2) and reference genes (TUBB, PP2A, HNR, ARF) from Pisum sativum and Avena sativa samples during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation) and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Columns: well – position on the qPCR plate, sample ID, sample type, target gene, target type (reference vs. target of interest), dye, Cq value. Additional columns represent raw fluorescence values across PCR cycles. The archive includes a README.txt file with annotations and specifications. File naming convention: OrderNumberOfSeries_Plate_RT-qPCR_ Replicate.File name: Avena_77K.tab – File (.csv format) contains raw data for low-temperature (77 K) chlorophyll or protochlorophyllide fluorescence emission spectra of Avena sativa first leaf tissue samples collected during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation), 3 hours of light illumination after 10 days of etiolation – 0d3hL and baseline in manner: OrderNumber_species_ ReplicateNumber | OrderNumber_baseline.File name: Pisum_77K.tab – File (.csv format) contains raw data for low-temperature (77 K) chlorophyll or protochlorophyllide fluorescence emission spectra of Pisum sativum first leaf tissue samples collected during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation), 3 hours of light illumination after 10 days of etiolation – 0d3hL and baseline in manner: OrderNumber_species_ ReplicateNumber | OrderNumber_baseline.File name: Pisum_densitometry.tab – File (.csv format) contains densitometric analysis of immunodetection of etioplast and chloroplast proteins (PsaA, PsaB, PsaC, PsaD, Lhca2) in samples of Pisum sativum collected during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation), 3 hours of light illumination after 10 days of etiolation – 0d3hL, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Column A – lane_no, column B – sample_description (naming convention: species_GrowthStage; columns C-Q – Adjusted Volume Intensity data with naming convention: Protein_ ReplicateNumber.File name: Avena_ densitometry.tab – File (.csv format) contains densitometric analysis of immunodetection of etioplast and chloroplast proteins (PsaA, PsaB, PsaC, PsaD, Lhca2) in samples of Avena sativa collected during 10 days of etiolation – (-10d – 0 days of etiolation, -7d - 3 days of etiolation, -5d – 5 days of etiolation, -3d - 7 days of etiolation, 0d - 10 days of etiolation), 3 hours of light illumination after 10 days of etiolation – 0d3hL, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Column A – lane_no, column B – sample_description (naming convention: species_GrowthStage; columns C-Q – Adjusted Volume Intensity data with naming convention: Protein_ ReplicateNumber.File name: species_densitometry.tab – File (.csv format) contains densitometric analysis of immunodetection of etioplast and chloroplast proteins (PsaA, PsaB, Ycf4, Alb3, Lhca2) in samples of Arabidopsis thaliana, Phaseolus coccineus, Pisum sativum, and Avena sativa collected after 10 days of etiolation – 0d, and 20 days of long day conditions (16 h light/8 h darkness) – 20dL. Column A – lane_no, column B – sample_description (naming convention: species_GrowthStage; columns C-Q – Adjusted Volume Intensity data wth naming convention: Protein_ ReplicateNumber.