cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2008.1966
title: House dust mite DNA vaccine candidate development of full length and poly-th1 epitopes

description: Allergy is an immune dysregulation against allergens that lead to T-helper type 2 (Th2) immune response and specific IgE production. House dust mite is the most common cause of airway allergic diseases of which 60-70% of patients have detectable anti-house dust mite IgE antibodies. Der p 1 (Dermatophagoides pteronyssinus) protein is one of the major HDM allergens that can sensitize the immune system in an allergic prone person for whom develops an allergic disease. Thus developing a safe and effective HDM vaccine to induce Th1 responses to down regulate Th2 responses is warranted. This study was to developed and optimize a house dust mite (HDM) Der p1 DNA vaccine to generate specific Th1-type responses as a prototype vaccine to be further evaluated its role as a HDM immunotherapy To develop an efficient DNA vaccine, humanized synthetic gene encoding mature Der p 1 allergen was cloned into pHIS plasmid vector containing a CpG motif. The CpG motif, toll-like receptor 9 (TLR9) ligand, served as an adjuvant to activate powerful specific Th1 responses. The protein expression of the construct was tested in pGFP-mHuDer p 1 transfected HEK cells and showed a detection of the chimeric GFP-Der p 1 protein. Intradermally immunizations of pHIS-mHuDer p 1 or control plasmids with and without liposome were performed in Balb/c mice. The immunogenicity was examined by ELISA assays. The results showed mice vaccinated with pHIS-mHuDer p 1 mounted Der p1 specific Th1 immune responses, as evidenced by the detection of specific IgG2a antibody, while it was not detected in control group. Of interest, mice immunized with lipoplexes (liposome-pHIS-mHuDer p 1 DNA) were more efficient to generate faster and higher Der p 1 specific IgG2a responses. An HDM allergic mouse model had also been developed, ProDer p 1 with alum was injected intraperitoneally into mice and had shown a specific Th2 response with IgG1 and IgE antibodies against Der p 1 protein. In conclusions, a humanized Der p1 DNA vaccine has been developed preclinically. Replacing dust mite codons to mammalian codon usage had increased protein expression and its immunogenicity significantly. Immunogenicity studies in mice, when the recombinant humanized Der p 1 DNA injection intradermally showed specific Th1 immune response. Moreover, the lipoplexes formulation was significantly increased the efficacy of the DNA vaccine. This candidate vaccine is therefore warranted for further development as a therapeutic vaccine for house dust mite allergy.
DOI: 10.14457/cu.the.2008.1967
title: Inhibitory effect of tetracyclines on pancreatic lipase activity

description: To demonstrate the inhibitory potencies of 4 chemical derivatives of tetracyclines consist of tetracycline, doxycycline, chlortetracycline and oxytetracycline. Tetracyclines are a group of broad-spectrum agents, exhibiting activity against a wide range of gram-positive and gram-negative bacteria. The results showed inhibitory potency of doxycycline (IC[subscript 50] = 55.42 +- 1.67 µM) &gt; chlortetracycline (IC[subscript 50] = 88.71 +- 11.39 µM) &gt; tetracycline (IC[subscript 50] &gt; 500 µM) but it had no inhibitory activity of oxytetracycline. However, the study which compared the potency between doxycycline and orlistat, a drug clinically used for weight loss by directly inhibit pancreatic lipase indicated that doxycycline was about 80 times less potent than orlistat (IC[subscript 50] = 1.31 +- 0.13 µM). The study inhibitions mechanism using Lineweaver-Burk plot showed that doxycycline was a reversible non-competitive type inhibition against pancreatic lipase which is differs from orlistat in which it was irreversible inhibition. The affinity inhibitor constant of doxycycline showed K[subscript i] = 66.41 +- 3.27 µM and K[subscript i]' = 70.49 +- 7.11 µM. Moreover, doxycycline exhibited the synergistic inhibition on pancreatic lipase activity when administration with a low dose of orlistat. The oral administration of doxycycline (20-100 mg/kg) significantly decreased serum triglyceride concentration in lipid emulsion-induced hyperlipidemia rats. In conclusion, doxycycline markedly decreased serum triglyceride concentration in rats by inhibiting pancreatic lipase. This research may be beneficial for further studies in clinical pharmacology and toxicology.
DOI: 10.14457/cu.the.2008.1968
title: Free-surface flows over submerged obstacles

description: We consider free-surface ﬂows over submerged obstacles under the eﬀects of gravity and surface tension. The ﬂuid is treated as inviscid and incompressible. The ﬂow is assumed to be steady, two-dimensional, and irrotational. The ﬂow is characterized by the following parameters: Froude number F, Bond number Bo, distance between the obstacles x[subscript d], and height of the obstacles hob. Fully nonlinear problem is solved numerically by using the boundary integral equation technique. After the discretization, we obtain a system of nonlinear algebraic equations which can be solved by the Newton’s method. In addition, the weakly nonlinear problem of steady and time-dependent ﬂows are investigated and compared with the fully nonlinear results. In this thesis, special case of ﬂows over a single obstacle is summarized in the solution diagram of (F, hob)-plane for bump(hob &gt; 0) and dip (hob &lt; 0). New family of solutions of free-surface ﬂows over two obstacles are proposed.
DOI: 10.14457/cu.the.2008.1969
title: Petrochemistry of igneous rocks in the southern parts of the Chatree, Changwat Pichit

description: The studied N and V prospects in the southern parts of Chatree Gold Mine, Changwat Pichit is located in the central part of the north-trending Permo-Triassic Loei-Petchabun-Nakhon Nayok Volcanic Belts. Two main types of igneous rocks are recognized petrographically, namely porphyritic granodiorite and andesite. The former show lines of evidence of intrusion into the latter. The porphyritic granodiorite is characterized by phenocrysts of plagioclase (An [subscript 30]), k-feldspar, quartz, hornblende and biotite set in fine-grained quartz-rich groundmass. Associated alterations are predominant potassic alteration with minority of phyllic alteration. Pyrite is principal ore in veins and veinlets whereas chalcopyrite and molybdenite are present in small amount. The andesite host is unvariably porphyritic and largely composed of altered plagioclase and hornblende phenocrysts set in glassy-microlite groundmass. The alteration of vocalnic rocks are widespread and is peripheral with propylitic alteration. Twenty-two least-altered and least-weathered samples of both plutonic and volcanic rocks were selected for mobile-and immobile-elemental analyses using XRF, ICP-OES and ICP-MS methods. Both petrographically and chemically, these studied igneous rocks can be classified as granodiorite to tonalite and basaltic andesite. Geochemically, the studied rocks belong to the calc-alkaline affinity occurring as volcanic arc granite and island arc tholeiite to volcanic arc basalt. Spider diagrams of trace and rare-earth elements reveal that granodiorite shows a pattern consistent with those of the Tertiary intrusive igneous rocks of Azerbaijan, Iran. Principal mineralization at the studies N and V prospects is believed to belong to porphyry-copper type occurring in the shallow intrusive environment and was formed as a result of oceanic subduction during Permo-Triassic Period between Lumpang-Chiang Rai and Nakhon Thai plates.
DOI: 10.14457/cu.the.2008.1970
title: Catalytic cracking of polypropylene and polyethylene using zirconium/aluminium oxide-pillared clay

description: Aluminium oxide-pillared clay was prepared by intercalation between ionic precursors and raw clay suspensions with any previous purification and homoionisation of clay prior to pillaring. The intercalated product was calcined at 500 degress celsius for 1h. And then was impregnated with various amounts of zirconium (from 0.0 to 5.0 wt%) by slurry method and calcined at 450 degress celsius for 4h. The basal spacing, the surface area, the aluminium contents and the vibrational of chemical bonds in different environments were determined by XRD, N[subscript 2] -BET, ICP-AES and IR respectively. The synthesized aluminium oxide-pillared clay had a basal spacing and surface area of 16.3 aungstrom and 189 m[superscript 2] g [superscript-1]. The basal spacings of zirconium doped aluminium oxide-pillared clays(zirconium/aluminium oxide-pillared clays) at 15.6 aungstrom were lower than aluminium oxide-pillared clay. Increasing amount of zirconium, the surface area decreased from 188 to 131 m [superscript 2] g [superscript -1]. When compared catalytic cracking reaction between two type of polymer observed that polyethylene more difficult than polypropylene. When aluminium oxide-pillared clay and zirconium/aluminium oxide-pillared clays were used as catalysts, the conversions of both polymers greatly increased compared to that in the absence of a catalyst. The cracking reaction was carried out in a semi-batch reactor with a mixture of catalyst and plastic at 350-450 degress celsius. The effects of reaction temperature, reaction time, type of catalyst and catalyst per plastic ratio were discussed. The activity and selectivity of catalysts modified by impregnation of zirconium were higher than the original clay catalyst. Gaseous and liquidous hydrocarbon products were analysed by GC. The optimal condition for PP cracking was reaction time of 1.0 h, reaction temperature of 380 degress celsius, 2.0wt%zirconium/aluminium oxide-pillared clay as a catalyst and 10.0 wt% catalyst per plastic ratio. The major components of gas fraction were C [subscript 5] + and C [subscript 3] (propene). Liquid fractions were in he range of C [subscript 7] to C [subscript 9] with the boiling point range compared to that of standard gasoline. The optimal condition for PE cracking was reaction time of 1.5 h, reaction temperature of 410 degress Celsius, 2.0 wt%zirconium/aluminium oxide-pillared clay and 10.0 % catalyst per plastic ratio. The major components of gas fractions were C [subscript 5+], C [subscript 4](n-butane), C [subscript 3] (propene), C [subscript 2] (ethane) and C [subscript 1] (methane). Liquid fractions were in he range of C [subscript 7] to C [subscript 10].
DOI: 10.14457/cu.the.2008.1971
title: Microstructural assessment of cubic InN film grown by molecular beam epitaxy

description: In the thesis, structural modification and crystal quality of cubic InN (c-InN) films grown on GaAs (001) substrates by molecular beam epitaxy have been systematically investigated and analyzed. The effects of growth conditions, namely In- and N-rich conditions, are established. Based on high-resolution X-ray diffraction, the result demonstrates that all the InN grown films have a cubic structure. On the other hand, electron diffraction (ED) pattern and cross-sectional transmission electron microscopy (TEM) micrographs demonstrate that the InN grown films contain a high density of stacking faults (SFs) parallel to (111) planes. Besides, the density of SF and twin defects decreases with the distance from the interface of c-InN/GaAs. No different type of single diffraction spots on the ED pattern was observed. On the other hand, TEM micrographs the ED patterns demonstrate that the c-InN films exhibited a transition from cubic to mixed cubic/hexagonal phase under N-rich growth conditions. In addition, µ-Raman scattering spectra obtained from these InN layers confirmed the existence of a structural modification from cubic to mixed cubic/hexagonal phase in microstructure of the N-rich layers, which exhibit higher hexagonal-phase incorporation than that of the In-rich layers. Our results suggest that the growth condition is a key parameter in the growth of high cubic phase purity c-InN films with lower incorporation of single-crystal h-InN.
DOI: 10.14457/cu.the.2008.1972
title: L-alanine production from the recombinant harboring alanine dehydrogenase and formate dehydrogenase genes and identification of a novel formate dehydrogenase

description: In this research, improvement of Escherichia coli host cell for high production of L-alanine and screening of a novel formate dehydrogenase (FDH) were performed. For improvement of host cell, the T7 gene 1 encoding T7 RNA polymerase was introduced into chromosome of E. coli MB2795, the racemase-deficiency strain, via transduction of lambda phage. After that, the constructed clone, namely E. coli KR, was used as a host cell for transformation of pETFA containing alanine dehydrogenase gene from Aeromonas hydrophila and formate dehydrogenase gene from Mycobacterium vaccae N10. The optical purity of L-alanine produced by recombinant clone of E. coli KR was over 95%. Surprisingly, a novel type of FDH, NADP+-FDH, was found in Burkholderia cepacia complex (BCC) during the screening of formate dehydrogenase. This FDH was able to use both NAD+ and NADP+ as coenzyme, however, it preferred NADP+ over NAD+. The distribution of formate dehydrogenase gene was determined among 46 strains from 10 species of the BCC. The gene was found to be present in several or all tested strains of B. cepacia, B. multivorans, B. cenocepacia, B. stabilis and B. pyrrocinia, but potentially absent in B. ambifaria, B. vietnamiensis, B. dolosa, B. anthina and B. ubonensis. The complete coding sequence of all FDH genes from the five species consisted of 1,161 bp encoding for a polypeptide of 386 amino acids. The similarity of amino acid sequences were very high (91-96%) among the five BCC and high 65-70% when compared to other bacterial FDHs. The gene encoding formate dehydrogenase from B. stabilis was cloned into E. coli BL21 (DE3) and the enzyme was purified to homogeneity. BstFDH had a molecular mass of subunit about 42 kDa. The optimum pH was ranged from 6.0-7.5 and optimum temperature was 60 degress Celsius No loss of the enzyme activity was observed upon incubation at 45, 50 and 55 degress Celsius for 16, 10 and 10 hr, respectively and the activity retained 50% after incubation for 36, 36 and 32 hr, respectively. The enzyme was stable over a broad pH ranged from 4.0 to 12.0. The apparent K [subscript m] values for formate, NADP+ and NAD+ were 62.5, 0.16 and 1.43 mM, respectively. Interestingly, all NAD+ -dependent FDHs and other D specific 2-hydroxy acid dehydrogenases contained the conserved coenzyme binding sequence Gly(Ala)XGlyXXGlyX17Asp for NAD+ while the NADP+ -dependent FDHs from the BCC possessed the sequence GlyXGlyXXGlyX17Gln. Therefore, the Gln223Asp single mutation of BstFDH was performed. Coenzyme preference of the mutant enzyme was completely changed from NADP+ to NAD+.
DOI: 10.14457/cu.the.2008.1973
title: Cloning and characterization of genes and proteins related to calcium metabolism of black tiger shrimp Penaeus monodon

description: Calcium ion (Ca[superscript 2+]) plays critical role in many cellular responses of eukaryotes. The important Ca[superscript 2+] controlling organelle (Ca[superscript 2+] homeostasis) in cellular level is the Endoplasmic reticulum (ER). Therefore, studying genes that control Ca[superscript 2+] homeostasis could lead to understand cellular responses related to Ca[superscript 2+]. The focus of this study was on calreticulin (CRT), Calnexin (CNX) and Endoplasmic Reticulum protein 57 (ERp57). These genes/proteins function together to control cellular Ca[superscript 2+] homeostasis and protein folding in the ER. The full length cDNA of these genes were successfully characterized. A single form of PmCRT and PmCNX contained open reading frame (ORF) of 1221 and 1788 bp corresponding to deduce proteins of 406 and 595 amino acid residues, respectively. In contrast, two isoforms of PmERp57 was found. They contained an identical ORF of 1458 bp (corresponding to 485 amino acid residues) but two different 3' UTR length of 529 and 713 bp. Furthermore, genomic organization of the three genes was characterized. The genomic PMCRT sequence contained 4 exons and 3 introns while that of PmERp57 consisted of 10 exons and 9 introns. Genomic organization of PmCNX was partially characterized. Only 4 exons and 3 introns were completed. Tissue distribution analysis of the three gene homologues revealed their expression in eye stalk, pleopod, gill, heart, ovaries, testis, hepatopancreas, stomach, intestine, hemocytes, thoracic ganglia, lympiod organ and antennal gland of P. monodon juveniles and broodstocks. Semi-quantitative RT-PCR were carried out to examine effect of thermal stress on expression levels of CRT, CNX and ERp57 in hemocytes, hepatopancreas and gill of the juvenile black tiger shrimps. Differential expression was found only in hemocytes. The semi-quantitative result from hemocytes was confirmed by quantitative real-time PCR. Expression of the three genes in hemocytes was up-regulated within the first hour after the 30 ºC heat treatment (P &lt; 0.05). After that, their expression levels return to the normal levels. Recombinant proteins of PmCRT, PmCNX and PmERp57 were produced using an E. coli system. All recombinant proteins were expressed in the soluble form. These recombinant proteins were purified and some protein functions were in vitro assayed. Binding of PMCNX to Ca[superscript 2+] caused change in its conformation, resulting in a mobility shift on native PAGE gel while the others did not exhibit the shift. Moreover, PmCRT and PmCNX also complexed with PmERp57, causing mobility shift of the proteins bands on native PAGE gel.
DOI: 10.14457/cu.the.2008.1974
title: Value relevance of EVA in mergers and acquisitions

description: This study investigates the value-relevance of the EVA (Economic Value Added) in the aspect that the EVA can outperform other accounting measures using mergers and acquisitions data. Relative and incremental information content tests are conducted to investigate whether EVA is more highly correlated with the takeover premium, acquirer excess return and combined return than other traditional accounting measures (CFO, EBEI, RI), and examine which components of EVA, if any, contribute to these association. Relative information content tests show that CFO is more highly correlated with the target premium and combined return while EVA can best describe the variation in acquirer abnormal return. However, these differences in explanatory power are not significant. For the incremental information content test, the results show that EVA components add only marginally to the information content (only CFO and Capital Charge (CapChg) which is not the unique component of EVA). Considered together, there is no enough evidence to claim that EVA outperforms other accounting measures in mergers and acquisitions.
DOI: 10.14457/cu.the.2008.1975
title: Synthesis and characterizaton of polyamidoamine dendrimer nanopolymers

description: The PAMAM dendrimers were synthesized by consecutively repeating two reactions; Michael addition and Amidation to produce amine terminated product. The FTIR, 1H and 13C-NMR analysis were employed to follow the synthesis reaction. In separate work, grafting of hyperbranched PAMAM polymers onto ultrafine silica followed by functionalization via the introduction of phosphonic acid groups into the branch ends was performed. First, an initiating site was incorporated into the silica surface by reacting the silica silanol group with 3-aminopropyltriethoxysilane, producing amino functionalized silica. The free amine group content was altered by varying the ratio of methanol to water in the hydrolysis step of the silanization reaction. Grafting of PAMAM was attained by three rounds of sequential Michael addition of silica amino groups to methyl acrylate and amidation of the resulting terminal methyl ester groups with ethylenediamine. Completion of the grafting reaction in each step was clearly confirmed using FTIR analysis. However, the amino group content determined in each step was found to be significantly lower than theoretically expected, perhaps indicative of side reactions and, in later stages, steric hindrance. The PAMAM dendrimers and hyperbranched PAMAM grafted silica were functionalized by a Mannich type reaction to phosphorylate the terminal amino groups, producing the phosphorylated PAMAM dendrimers and phosphorylated hyperbranched PAMAM grafted silica. Then they were applied on cotton fabric to produce flame-retardant cellulose. The two synthesized products showed flame retardancy effect on cotton fabric. After washing test, the phosphorylated hyperbranched PAMAM grafted silica showed better retention of fire retardancy than phosphorylated PAMAM dendrimers, indicating its wash-off resistancy performance. In addition, the bursting strength of phosphorylated PAMAM dendrimers finished cotton was worst than phosphorylated hyperbranched PAMAM grafted silica finished cotton. This might be due to the effect of more phosphonic acid groups content of phosphorylated PAMAM dendrimers. As a result, its higher acidicity accelerated the thermal degradation of cotton fabric during fixation of phosphorylated PAMAM dendrimers.
DOI: 10.14457/cu.the.2008.1976
title: Use of HIVQUAL-T program to access quality of care among HIV patients at community hospitals, Nakhonratchasima Province

description: The objectives of this study were 1) to explore the completeness of HIV/AIDS patients care databases in community hospitals, 2) to assess quality of care in HIV/AIDS patients by the HIVQUAL-T program version 4.0 in HIV/AIDS patients, based on the guidelines of National Health Security Office for HIV/AIDS management, and 3) to propose a strategy for the continuous improvement of quality of care in HIV/AIDS patients. The study assessed the completeness of HIV/AIDS patient’s data in the NAPHA program and medical record from community hospitals during October 2006 to September 2007. The study also conducted a group meeting of the representation of ARV team in community hospitals to propose a strategy to improve quality of care of HIV/AIDS patients. The results showed that only 6 from 26 hospitals updated and completed data which covered all 7 indicators. There were 262 HIV/AIDS patients including 138 females and 124 males. All of them received ARV drugs. After assessing quality of care by HIVQUAL-T, 37.4% of patients had CD4 counts monitored once a year, 43.5% of patients had CD4 counts checked at least twice a year, 1.91% patients had viral load monitored once a year. For OI prophylaxis, 95% of patients received PCP prophylaxis and 83% of patients received Cryptococcosis prophylaxis. None of them received MAC prophylaxis. All patients with TB received TB treatment but none of them had a PPD skin test during the study. Only 6.49% of patients had a VDRL test for syphilis screening and 10.87% of female HIV/AIDS patients received PAP smear screening. The results of overall quality assessments were reviewed by the representative of ARV team in community hospitals to propose a strategy to improve quality of care of the HIV/AIDS patients. They proposed to have the individual patient record form that gathered all HIV care data. They also proposed the systematic management of quality of care assessment, to encourage HIV/AIDS patients to participate in quality assessment monitoring, and continuous quality of care assessment by the ARV team.