DOI: 10.22002/6rt8a-5q207
title: hns_overexpression_PS152

description: Andrew's DNA project
description: <strong>Tilt Series Date:</strong> 2016-08-24</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown for 27h from glycerol stock. At OD 0.51 induced with ~3mM IPTG and grown for 4h and froze as such, no spinning. OD was 0.52. Cell density and distribution looked good.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-49/rawdata/</p>hnsM9_PS152_atlas10007.mrc,  Tilt Series (Pixel Size 0.6 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-49/rawdata/hnsM9_PS152_atlas10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        hnsM9_PS152_atlas10007_full.rec,  Reconstruction (Pixel Size 2.4 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2016-08-24-49/3dimage_78551/hnsM9_PS152_atlas10007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2016-08-24-49_slicer17037.png,  stripes / flow-lines within the  .  REZ / nucleoid / .  ribosome exclusion zone .  -andrew, 810.3 kB  
        
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        psa2016-08-24-49_slicer17038.png,  stripes / flow-lines within the  .  REZ / nucleoid / .  ribosome exclusion zone .  -andrew, 812.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-49_slicer17038.png"
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        psa2016-08-24-49_slicer17039.png,  stripes / flow-lines within the  .  REZ / nucleoid / .  ribosome exclusion zone .  -andrew, 468.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-49_slicer17039.png"
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        psa2016-08-24-49_slicer17040.png,  magenta arrow: dense fibrous structure (probably DNA)  green arrow:  .  stripes  flow-lines within the .  REZ / nucleoid  -andrew, 474.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2016-08-24-49_slicer17040.png"
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