cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7583570
title: Attenuated <i>Salmonella</i> Typhimurium expressing <i>Salmonella</i> Paratyphoid A O-antigen induces protective immune responses against two <i>Salmonella</i> strains

description: <i>Salmonella enterica</i> serovar Paratyphi A is the main causative agent of paratyphoid fever in many Asian countries. As paratyphoid is spread by the fecal-oral route, the most effective means of controlling <i>S</i>. Paratyphi A infection is through the availability of clean water supplies and working sanitation services. Because sanitation facilities improve slowly in these poor areas and antibiotic resistance is severe, the development of a safe and effective vaccine remains a priority for controlling the spread of paratyphoid disease. In this study, we investigated the strategy of heterologous O-antigenic O2 serotype (<i>S</i>. Paratyphi A characterized) conversion in <i>S</i>. Typhimurium to prevent paratyphoid infections. A series of <i>S</i>. Typhimurium mutants were constructed with replacement of <i>abe, wzx</i><sub>B1</sub> and <i>wbaV</i><sub>B1</sub> genes with respective <i>prt-tyv</i><sub>A1</sub>, <i>wzx</i><sub>A1</sub> and <i>wbaV</i><sub>A1</sub>, and the results showed that only three genes including <i>prt, wbaV<sub>A1</sub></i> and <i>wzx<sub>A1</sub></i> from <i>S</i>. Paratyphi A presence enable <i>S</i>. Typhimurium to sufficiently express O2 antigen polysaccharide. We also constructed a series of live attenuated <i>S</i>. Typhimurium vaccine candidates expressing heterologous O2 O-antigens, and a mouse model was used to evaluate the immunogenicity of live vaccines. ELISA data showed that vaccine candidates could induce a comparatively high level of <i>S</i>. Paratyphi A and/or <i>S</i>. Typhimurium LPS-specific IgG and IgA responses in murine model, and IgG2a levels were consistently higher than IgG1 levels. Moreover, the functional properties of serum antibodies were evaluated using <i>in vitro</i> C3 complement deposition and opsonophagocytic assays. Our work highlights the potential for developing <i>S</i>. Typhimurium live vaccines against <i>S</i>. Paratyphi A.
DOI: 10.6084/m9.figshare.7583570.v2
title: Attenuated <i>Salmonella</i> Typhimurium expressing <i>Salmonella</i> Paratyphoid A O-antigen induces protective immune responses against two <i>Salmonella</i> strains

description: <i>Salmonella enterica</i> serovar Paratyphi A is the main causative agent of paratyphoid fever in many Asian countries. As paratyphoid is spread by the fecal-oral route, the most effective means of controlling <i>S</i>. Paratyphi A infection is through the availability of clean water supplies and working sanitation services. Because sanitation facilities improve slowly in these poor areas and antibiotic resistance is severe, the development of a safe and effective vaccine remains a priority for controlling the spread of paratyphoid disease. In this study, we investigated the strategy of heterologous O-antigenic O2 serotype (<i>S</i>. Paratyphi A characterized) conversion in <i>S</i>. Typhimurium to prevent paratyphoid infections. A series of <i>S</i>. Typhimurium mutants were constructed with replacement of <i>abe, wzx</i><sub>B1</sub> and <i>wbaV</i><sub>B1</sub> genes with respective <i>prt-tyv</i><sub>A1</sub>, <i>wzx</i><sub>A1</sub> and <i>wbaV</i><sub>A1</sub>, and the results showed that only three genes including <i>prt, wbaV<sub>A1</sub></i> and <i>wzx<sub>A1</sub></i> from <i>S</i>. Paratyphi A presence enable <i>S</i>. Typhimurium to sufficiently express O2 antigen polysaccharide. We also constructed a series of live attenuated <i>S</i>. Typhimurium vaccine candidates expressing heterologous O2 O-antigens, and a mouse model was used to evaluate the immunogenicity of live vaccines. ELISA data showed that vaccine candidates could induce a comparatively high level of <i>S</i>. Paratyphi A and/or <i>S</i>. Typhimurium LPS-specific IgG and IgA responses in murine model, and IgG2a levels were consistently higher than IgG1 levels. Moreover, the functional properties of serum antibodies were evaluated using <i>in vitro</i> C3 complement deposition and opsonophagocytic assays. Our work highlights the potential for developing <i>S</i>. Typhimurium live vaccines against <i>S</i>. Paratyphi A.
DOI: 10.6084/m9.figshare.7583570.v1
title: Attenuated <i>Salmonella</i> Typhimurium expressing <i>Salmonella</i> Paratyphoid A O-antigen induces protective immune responses against two <i>Salmonella</i> strains

description: <i>Salmonella enterica</i> serovar Paratyphi A is the main causative agent of paratyphoid fever in many Asian countries. As paratyphoid is spread by the fecal-oral route, the most effective means of controlling <i>S</i>. Paratyphi A infection is through the availability of clean water supplies and working sanitation services. Because sanitation facilities improve slowly in these poor areas and antibiotic resistance is severe, the development of a safe and effective vaccine remains a priority for controlling the spread of paratyphoid disease. In this study, we investigated the strategy of heterologous O-antigenic O2 serotype (<i>S</i>. Paratyphi A characterized) conversion in <i>S</i>. Typhimurium to prevent paratyphoid infections. A series of <i>S</i>. Typhimurium mutants were constructed with replacement of <i>abe, wzx</i><sub>B1</sub> and <i>wbaV</i><sub>B1</sub> genes with respective <i>prt-tyv</i><sub>A1</sub>, <i>wzx</i><sub>A1</sub> and <i>wbaV</i><sub>A1</sub>, and the results showed that only three genes including <i>prt, wbaV<sub>A1</sub></i> and <i>wzx<sub>A1</sub></i> from <i>S</i>. Paratyphi A presence enable <i>S</i>. Typhimurium to sufficiently express O2 antigen polysaccharide. We also constructed a series of live attenuated <i>S</i>. Typhimurium vaccine candidates expressing heterologous O2 O-antigens, and a mouse model was used to evaluate the immunogenicity of live vaccines. ELISA data showed that vaccine candidates could induce a comparatively high level of <i>S</i>. Paratyphi A and/or <i>S</i>. Typhimurium LPS-specific IgG and IgA responses in murine model, and IgG2a levels were consistently higher than IgG1 levels. Moreover, the functional properties of serum antibodies were evaluated using <i>in vitro</i> C3 complement deposition and opsonophagocytic assays. Our work highlights the potential for developing <i>S</i>. Typhimurium live vaccines against <i>S</i>. Paratyphi A.
DOI: 10.6084/m9.figshare.21739348
title: Impact of implant-based breast reconstruction on bra fit

description: Comfortable and well-fitting bras are necessary for good quality of life but hard to find for women who undergo reconstruction after breast cancer treatment. This study aimed to provide data to inform bra designs for breast cancer survivors. We measured anatomical distances used in bra design on 3D clinical photographs of patients who underwent unilateral and bilateral implant-based reconstruction to quantify changes after reconstruction relative to the measured values before the person underwent surgery. We performed additional assessments of symmetry before surgery and after reconstruction, and we used regression analyses to identify associations between the measurements and patient characteristics, such as BMI. Overall, almost all measurements changed significantly in implant-based reconstructed breasts relative to native breasts. We highlight several aspects of ergonomic bra design that will be impacted by the changes in anatomical distances. <b>Practitioner summary:</b> Implant-based breast reconstruction surgery changes the breast so that off-the-rack bras are inadequate. This study provides designers with measurement data from women who underwent implant-based reconstruction to inform bra designs for this population. The key factor designers need to account for is the semi-spherical shape of the reconstructed breast.
DOI: 10.6084/m9.figshare.21739348.v2
title: Impact of implant-based breast reconstruction on bra fit

description: Comfortable and well-fitting bras are necessary for good quality of life but hard to find for women who undergo reconstruction after breast cancer treatment. This study aimed to provide data to inform bra designs for breast cancer survivors. We measured anatomical distances used in bra design on 3D clinical photographs of patients who underwent unilateral and bilateral implant-based reconstruction to quantify changes after reconstruction relative to the measured values before the person underwent surgery. We performed additional assessments of symmetry before surgery and after reconstruction, and we used regression analyses to identify associations between the measurements and patient characteristics, such as BMI. Overall, almost all measurements changed significantly in implant-based reconstructed breasts relative to native breasts. We highlight several aspects of ergonomic bra design that will be impacted by the changes in anatomical distances. <b>Practitioner summary:</b> Implant-based breast reconstruction surgery changes the breast so that off-the-rack bras are inadequate. This study provides designers with measurement data from women who underwent implant-based reconstruction to inform bra designs for this population. The key factor designers need to account for is the semi-spherical shape of the reconstructed breast.
DOI: 10.6084/m9.figshare.11777904
title: Supplementary figures from “Metabolite Identification, Tissue Distribution, Excretion and Preclinical Pharmacokinetic Studies of ET-26-HCl, a New Analog of Etomidate”

description: ET-26-HCl, a novel anaesthetic agent with promising pharmacological properties, lacks extensive studies on pharmacokinetics and disposition <i>in vitro</i> and <i>in vivo</i>. In this study, we investigated the metabolic stability, metabolite production and plasma protein binding (PPB) of ET-26-HCl along with its tissue distribution, excretion and pharmacokinetics in animals after intravenous administration. Ultra-high performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry identified a total of eight new metabolites after ET-26-HCl biotransformation in liver microsomes from different species. A hypothetical cytochrome P450-metabolic pathway including dehydrogenation, hydroxylation and demethylation was proposed. The PPB rate was highest in mouse and lowest in human. After intravenous administration, ET-26-HCl distributed rapidly to all tissues in rats and beagle dogs, with the highest concentrations in fat and liver. High concentrations of ET-26-acid, a major hydroxylation metabolite of ET-26-HCl, were found in liver, plasma and kidney. Almost complete clearance of ET-26-HCl from plasma occurred within 4 h after administration. Only a small fraction of the parent compound and its acid form were excreted via the urine and faeces. Taken together, the results added to a better understanding of the metabolic and pharmacokinetic properties of ET-26-HCl, which may contribute to the further development of this drug.
DOI: 10.6084/m9.figshare.11777904.v2
title: Supplementary figures from “Metabolite Identification, Tissue Distribution, Excretion and Preclinical Pharmacokinetic Studies of ET-26-HCl, a New Analog of Etomidate”

description: ET-26-HCl, a novel anaesthetic agent with promising pharmacological properties, lacks extensive studies on pharmacokinetics and disposition <i>in vitro</i> and <i>in vivo</i>. In this study, we investigated the metabolic stability, metabolite production and plasma protein binding (PPB) of ET-26-HCl along with its tissue distribution, excretion and pharmacokinetics in animals after intravenous administration. Ultra-high performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry identified a total of eight new metabolites after ET-26-HCl biotransformation in liver microsomes from different species. A hypothetical cytochrome P450-metabolic pathway including dehydrogenation, hydroxylation and demethylation was proposed. The PPB rate was highest in mouse and lowest in human. After intravenous administration, ET-26-HCl distributed rapidly to all tissues in rats and beagle dogs, with the highest concentrations in fat and liver. High concentrations of ET-26-acid, a major hydroxylation metabolite of ET-26-HCl, were found in liver, plasma and kidney. Almost complete clearance of ET-26-HCl from plasma occurred within 4 h after administration. Only a small fraction of the parent compound and its acid form were excreted via the urine and faeces. Taken together, the results added to a better understanding of the metabolic and pharmacokinetic properties of ET-26-HCl, which may contribute to the further development of this drug.
DOI: 10.6084/m9.figshare.7583675
title: Revising the systematic position of the extinct family Daohugoidae (basal Hymenoptera)

description: Two new species, <i>Daohugoa longa</i> Wang, Rasnitsyn & Ren sp. nov. and <i>D. bella</i> Wang, Rasnitsyn & Ren sp. nov. are described from the latest Middle Jurassic Jiulongshan Formation in north-eastern China. The new fossil evidence indicates that <i>Daohugoa</i> retains numerous plesiomorphies of basal Hymenoptera: an enlarged first flagellomere, complete xyelid-like venation, detached and folded laterotergites, and a laterally flattened sword-like ovipositor. Based on the combination of fossil and extant taxa, a phylogenetic analysis was conducted to clarify and ascertain the phylogenetic position of the extinct family Daohugoidae, which was originally classified in the superfamily Siricoidea. <i>Daohugoa</i> displays a contradictory array of characters. Its putative phylogenetic position within Hymenoptera is discussed in detail with reference to characters associated with the antenna, pronotum, wing venation, mesonotum and ovipositor. Based on the results from phylogenetic analysis and a combination of unique key features, we propose to include the group as a subfamily – Daohugoinae – within Xyelidae. These new findings increase our awareness of Mesozoic basal hymenopterans, and enhance our understanding of the evolution of morphological key characters for basal Hymenoptera. http://zoobank.org/urn:lsid:zoobank.org:pub:E6836213-132F-499A-9413-7C8971E2D5BF
DOI: 10.6084/m9.figshare.7583675.v2
title: Revising the systematic position of the extinct family Daohugoidae (basal Hymenoptera)

description: Two new species, <i>Daohugoa longa</i> Wang, Rasnitsyn & Ren sp. nov. and <i>D. bella</i> Wang, Rasnitsyn & Ren sp. nov. are described from the latest Middle Jurassic Jiulongshan Formation in north-eastern China. The new fossil evidence indicates that <i>Daohugoa</i> retains numerous plesiomorphies of basal Hymenoptera: an enlarged first flagellomere, complete xyelid-like venation, detached and folded laterotergites, and a laterally flattened sword-like ovipositor. Based on the combination of fossil and extant taxa, a phylogenetic analysis was conducted to clarify and ascertain the phylogenetic position of the extinct family Daohugoidae, which was originally classified in the superfamily Siricoidea. <i>Daohugoa</i> displays a contradictory array of characters. Its putative phylogenetic position within Hymenoptera is discussed in detail with reference to characters associated with the antenna, pronotum, wing venation, mesonotum and ovipositor. Based on the results from phylogenetic analysis and a combination of unique key features, we propose to include the group as a subfamily – Daohugoinae – within Xyelidae. These new findings increase our awareness of Mesozoic basal hymenopterans, and enhance our understanding of the evolution of morphological key characters for basal Hymenoptera. http://zoobank.org/urn:lsid:zoobank.org:pub:E6836213-132F-499A-9413-7C8971E2D5BF
DOI: 10.6084/m9.figshare.7583675.v1
title: Revising the systematic position of the extinct family Daohugoidae (basal Hymenoptera)

description: Two new species, <i>Daohugoa longa</i> Wang, Rasnitsyn & Ren sp. nov. and <i>D. bella</i> Wang, Rasnitsyn & Ren sp. nov. are described from the latest Middle Jurassic Jiulongshan Formation in north-eastern China. The new fossil evidence indicates that <i>Daohugoa</i> retains numerous plesiomorphies of basal Hymenoptera: an enlarged first flagellomere, complete xyelid-like venation, detached and folded laterotergites, and a laterally flattened sword-like ovipositor. Based on the combination of fossil and extant taxa, a phylogenetic analysis was conducted to clarify and ascertain the phylogenetic position of the extinct family Daohugoidae, which was originally classified in the superfamily Siricoidea. <i>Daohugoa</i> displays a contradictory array of characters. Its putative phylogenetic position within Hymenoptera is discussed in detail with reference to characters associated with the antenna, pronotum, wing venation, mesonotum and ovipositor. Based on the results from phylogenetic analysis and a combination of unique key features, we propose to include the group as a subfamily – Daohugoinae – within Xyelidae. These new findings increase our awareness of Mesozoic basal hymenopterans, and enhance our understanding of the evolution of morphological key characters for basal Hymenoptera. http://zoobank.org/urn:lsid:zoobank.org:pub:E6836213-132F-499A-9413-7C8971E2D5BF
DOI: 10.6084/m9.figshare.24873976.v1
title: Supplementary Data S3 from Bare parts in the Galliformes: the evolution of a multifunctional structure

description: All life history traits and respective references
DOI: 10.6084/m9.figshare.12291368
title: Additional file 3 of The association of MEFV gene mutations with the disease risk and severity of systemic juvenile idiopathic arthritis

description: Additional file 3. Raw data of the sequencing results.
DOI: 10.5281/zenodo.10474319
title: High-resolution Annual Dynamic dataset of Curve Number from 2008 to 2021 over Conterminous United States

description: The spatial distribution and data quality of curve number (CN) values determine the performance of hydrological estimations. However, existing CN datasets are constrained by universal-applicability hypothesis, medium resolution, and imbalance between specificity CN tables to generalized land use/land cover (LULC) maps, which hinder their applicability and predictive accuracy. A new annual CN dataset named CUSCN30, featuring an enhanced resolution of 30 meters and accounting for temporal variations in climate and LULC in the continental United States (CONUS) between 2008 and 2021, was developed in this study. CUSCN30 demonstrated good performance in surface runoff estimation using CN method when compared to observed surface runoff for the selected watersheds. Compared with existing CN datasets, CUSCN30 exhibits the highest accuracy in runoff estimation for both normal and extreme rainfall events. In addition, CUSCN30, with its high spatial resolution, better captures the spatial heterogeneity of watersheds. This developed CN dataset can be used as input for hydrological models or machine learning algorithms to simulate rainfall-runoff across multiple spatiotemporal scales.
DOI: 10.25402/fon.19107047
title: Supplementary Tables – Developing an immune signature for triple‑negative breast cancer to predict prognosis and immune checkpoint inhibitor

description: <b>Supplementary
 Table S1</b>. Developing an immune
 signature for triple‑negative breast cancer to predict prognosis and
 immune checkpoint inhibitor
 <i>R packages in the
 study</i>
 
 <b>Supplementary
 Table S2</b>. Developing an immune
 signature for triple‑negative breast cancer to predict prognosis and
 immune checkpoint inhibitor
 <i>66 prognostic IRGs
 of IPRS</i>
 
 <b>Supplementary
 Table S3</b>. Developing an immune
 signature for triple‑negative breast cancer to predict prognosis and
 immune checkpoint inhibitor
 <i>Multivariate Cox
 regression for IPRS</i>
 
 <b>Supplementary Table
 S4</b>. Developing an immune signature
 for triple‑negative breast cancer to predict prognosis and
 immune checkpoint inhibitor
 <i>Pathway enrichment
 analysis for 66 IRGs including KEGG and GO database</i>
DOI: 10.6084/m9.figshare.20147038
title: Additional file 13 of circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

description: Additional file 13: Supplementary Table S4. Proteins might bind to circEXOC6B were identified by mass spectrometry (MS).
DOI: 10.6084/m9.figshare.14912675.v1
title: Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells

description: Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in cell-cell and tissue-tissue communication. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived EVs on CSPCs proliferation and differentiation. The characteristics of ADSCs-derived EVs were identified, and proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. SEM, histological staining, biochemical and biomechanical analysis was performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40–1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induce CSPCs to produce significantly more cartilage matrix and proteoglycan. In conclusion, hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering.
DOI: 10.6084/m9.figshare.14912675
title: Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells

description: Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in cell-cell and tissue-tissue communication. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived EVs on CSPCs proliferation and differentiation. The characteristics of ADSCs-derived EVs were identified, and proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. SEM, histological staining, biochemical and biomechanical analysis was performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40–1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induce CSPCs to produce significantly more cartilage matrix and proteoglycan. In conclusion, hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering.