DOI: 10.5061/dryad.b245q
title: Data from: A molecular diagnostic tool for the preliminary assessment of host-parasitoid associations in biological control programmes for a new invasive pest

description: Evaluation of host-parasitoid associations can be tenuous using
 conventional methods. Molecular techniques are well-placed to identify
 trophic links and resolve host-parasitoid associations. Establishment of
 the highly invasive Brown Marmorated Stink Bug, Halyomorpha halys
 (Hemiptera: Pentatomidae) outside Asia has prompted interest in the use of
 egg parasitoids (Hymenoptera: Scelionidae) as biological control agents.
 However, little is known regarding their host ranges. To address this, a
 DNA barcoding approach was taken wherein general PCR primers for
 Scelionidae and Pentatomidae were developed to amplify and sequence
 &gt;500-bp products within the DNA barcoding region of the Cytochrome
 Oxidase I (COI) gene that would permit identification of key players in
 this association. Amplification of DNA from Pentatomidae and Scelionidae
 was consistent across a broad range of taxa within these families, and
 permitted detection of Scelionidae eggs within H. halys 1h following
 oviposition. In laboratory assays, amplification and sequencing of DNA
 from empty, parasitized eggs was successful for both host (100% success)
 and parasitoid (50% success). When applied to field-collected, empty egg
 masses the primers permitted host identification in 50 – 100% of the eggs
 analyzed, and yielded species-level identifications. Parasitoid
 identification success ranged from 33 – 67% among field-collected eggs,
 with genus-level identification for most specimens. The inability to
 obtain species-level identities for these individuals is due to the lack
 of coverage of this taxonomic group in public DNA sequence databases; this
 situation is likely to improve as more species are sequenced and recorded
 in these databases. These primers were able to detect and identify both
 pentatomid host and scelionid parasitoid in a hyperparasitized egg mass,
 thereby clarifying trophic links otherwise unresolved by conventional
 methodology.
description: Alignment of public
 sequences for the COI gene for the PentatomidaeAlignment of public
 sequences available on the Barcode of Life Datasystems (BOLD) for the DNA
 barcoding region of the COI gene for the Pentatomidae. Alignment was
 performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID
 number and Genus name provided for each specimen.01-Pentatomidae_BOLD_Public_Seq_Align.pdfAlignment of public sequences for the COI gene for the ScelionidaeAlignment of public sequences available on the Barcode of Life Datasystems (BOLD) for the DNA barcoding region of the COI gene for the Scelionidae. Alignment was performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID number and Genus name provided for each specimen.02-Scelionidae_BOLD_Public_Seq_Align.pdfAlignment of COI barcode sequences for Telenomus spp. (Hymenoptera: Scelionidae)Alignment of sequences for the DNA barcoding region of the COI gene for species of Telenomus collected in the field in Canada, Switzerland, and China. Alignment was performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID number and Genus name provided for each specimen.03-Telenomus_my_BOLD_Seq_Align.pdfAlignment of COI barcode sequences for Trissolcus spp. (Hymenoptera: Scelionidae)Alignment of sequences for the DNA barcoding region of the COI gene for species of Trissolcus collected in the field in Canada, Switzerland, and China. Alignment was performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID number and Genus name provided for each specimen.04-Trissolcus_My_BOLD_SEQ_Align.pdfin silico testing of primers for Scelionidae and PentatomidaeOutput for in silico testing of Scel_F1, PENT_F2, HCO-2198 on Scelionidae, Telenomus, Trissolcus, and Pentatomidae consensus sequences. Results generated using FastPCR v6.4 (Kalendar et al. 2011; http://primerdigital.com/fastpcr.html) to screen target sequences to determine primer location, orientation, efficiency of binding, and complementarity.in_silico_primer_test_all.pdfPrimer Picking Results for PentatomidaeLocation, direction, suitability, and amplification parameters for primers designed to amplify Pentatomidae. Results generated using Primer3 v0.4.0 (Untergrasser et al. 2012; http://bioinfo.ut.ee/primer3-0.4.0/).PRIMER PICKING RESULTS FOR PENTATOMIDAE.pdfPrimer Picking Results for ScelionidaeLocation, direction, suitability, and amplification parameters for primers designed to amplify Scelionidae. Primer stats generated using Primer3 v0.4.0 (Untergrasser et al. 2012; http://bioinfo.ut.ee/primer3-0.4.0/).PRIMER PICKING RESULTS FOR SCELIONIDAE.pdf