cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8987852
title: New species of Pyrrhulina (Teleostei: Characiformes: Lebiasinidae) from the eastern Amazon, Pará, Brazil

description: ABSTRACT A new species of Pyrrhulina is described based on 60 specimens from tributaries of the rio Amazonas: rio Anapu, rio Capim, rio Guamá and rio Xingu, and coastal drainages in the state of Pará, Brazil. The new species differs from all congeners by having the primary stripe (the horizontal stripe of dark pigmentation extending posteriorly from the snout) terminating at the distal edge of the opercle. In all other species of Pyrrhulina, the primary stripe is either restricted to the snout or continues beyond the head (i.e. at least the anteriormost four scales of the lateral line series).
DOI: 10.6084/m9.figshare.8987852.v1
title: New species of Pyrrhulina (Teleostei: Characiformes: Lebiasinidae) from the eastern Amazon, Pará, Brazil

description: ABSTRACT A new species of Pyrrhulina is described based on 60 specimens from tributaries of the rio Amazonas: rio Anapu, rio Capim, rio Guamá and rio Xingu, and coastal drainages in the state of Pará, Brazil. The new species differs from all congeners by having the primary stripe (the horizontal stripe of dark pigmentation extending posteriorly from the snout) terminating at the distal edge of the opercle. In all other species of Pyrrhulina, the primary stripe is either restricted to the snout or continues beyond the head (i.e. at least the anteriormost four scales of the lateral line series).
DOI: 10.6084/m9.figshare.8987846
title: Population and reproductive parameters of the red-tailed catfish, Phractocephalus hemioliopterus (Pimelodidae: Siluriformes), from the Xingu River, Brazil

description: ABSTRACT A field study of the sex ratio, length-weight ratio, body size at first gonadal maturation, and the breeding season of the red-tailed catfish, Phractocephalus hemioliopterus, was carried out in the region of the middle Xingu River in the eastern Amazon basin. The fish were sampled quarterly from September 2012 to January 2014 using gillnets and long lines. The sex ratio was balanced, and while males and females were similar in standard length, the females were heavier than the males, on average. The males presented negative allometric growth, and the females, isometric growth. The estimated size at first maturity was 77.8 cm. The variation in the gonadosomatic index and the greater frequencies of mature specimens indicated a reproductive peak at the beginning of the high-water (flood) period. As P. hemioliopterus is an important commercial fish, our findings will contribute to the future development of effective conservation and management strategies, such as minimum catch size and a close season that is appropriate for the region. These data will also provide important insights for the evaluation of potential impacts on the biology of this fish resulting from the construction of Belo Monte dam.
DOI: 10.6084/m9.figshare.8987849.v1
title: Unravelling the foraging behavior of the southern stingray, Hypanus americanus (Myliobatiformes: Dasyatidae) in a Southwestern Atlantic MPA

description: ABSTRACT Previous study suggested that the southern stingray (Hypanus americanus) displays a marked ontogenetic shift in its habitat use in the Fernando de Noronha Archipelago (FNA), an attractive touristic spot in Brazil, that could be associated with changes in its feeding habits and foraging strategies. Here, we conducted underwater visual surveys (~100 h a 3 years’ study) at this site to describe the foraging behavior of the same species in details and to assess diel patterns in activity. We observed that H. americanus is capable of several complex and highly plastic behaviors (we described more than 21 of them) while foraging. The foraging events’ timing appears to be related to the size (as well as sex) of the stingrays, environment and depth. In addition, our results support a segregation of habitat mediated by the size and sex of the stingrays, but it is able to modulate its foraging repertoire based mainly on environmental stimulus. Finally, we found that H. americanus forage intermittently throughout the day and tidal cycle, yet are less active during non-crepuscular periods and at flood and ebb tides. These results, coupled with the fact that the species uses both reef and beach environments, should be considered by decision makers in management plans.
DOI: 10.6084/m9.figshare.8987858.v1
title: Optimized protocol to isolate high quality genomic DNA from different tissues of a palm species

description: ABSTRACT The application of molecular techniques to tackle ecological and evolutionary questions requires genomic DNA in good quality and quantity. The quality of the isolated DNA, however, can be influenced by the tissue type and the way the sample was conserved and manipulated. Therefore, customizing protocols to improve the DNA isolation and locus amplification is crucial. We optimized a cheap and manual protocol of DNA extraction and microsatellites amplification using five different tissues of a palm species of the brazilian Atlantic Forest. We successfully extracted DNA from all five tissue types. Leaf, stem, and endocarp of non-dispersed seeds presented the highest rates of successful DNA extraction and microsatellite amplification; whereas root, endocarp of dispersed seeds, and embryo showed the lowest quality and quantity. Based on these results, we discussed the implications of using different tissues for studies about seed dispersal, pollination, and population genetics.
DOI: 10.6084/m9.figshare.8987837
title: Seed physiological quality and harvest point of dovyalis fruits

description: ABSTRACT The main propagation mode of dovyalis is by seeds, what makes important to determine the ideal moment of fruit harvest. This study aimed to evaluate the physiological seed quality and harvest time of dovyalis fruits. To determine the fruit physical attributes, they were visually classified into five classes, according to the maturity stages, based on the external color of the exocarp: stage 1: green fruit; stage 2: yellowish-green; stage 3: yellowish-brown; stage 4: brown; stage 5: intense brown. The seed extraction to evaluate the physiological quality was based on the seed water content, germination percentage, germination speed index and determination of the dry biomass of the shoot, root and seedlings originated from these seeds. The experimental design was completely randomized, containing 25 seeds per treatment and 4 replicates. The fruit color during ripening varies from green to intense brown. The fruits reach their largest size and fresh biomass accumulation in the stage 4, with the brown exocarp color represented in the Munsell chart by 2.5YR 3/4. The removal of seeds for propagation should occur from the stage 2 (2.5GY 5/8), when the fruits have a yellowish-green color, although no statistical variation is observed in the number of seeds during maturation.
DOI: 10.6084/m9.figshare.8987840
title: GLA Gene Mutation in Hypertrophic Cardiomyopathy with a New Variant Description: Is it Fabry's Disease?

description: Abstract Background: Fabry disease (FD) is an X-linked lysosomal storage disorder caused by mutations in the alpha galactosidase A gene (GLA) that lead to the enzymatic deficiency of alpha galactosidase (α-Gal A), resulting in the accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3), causing multiple organ dysfunctions. Objective: To perform GLA gene screening in a group of patients with echocardiographic diagnosis of hypertrophic cardiomyopathy (HCM). Methods: a cross-sectional study was conducted with HCM patients from a university hospital. Patients with coronary artery disease and valvulopathies were excluded. Mutation analysis of the GLA gene was performed. In male subjects, the analysis was performed after evidence of low α-Gal A activity. Results: 60 patients with echocardiographic diagnosis of HCM were included. Age ranged from 12 to 85 years and 60% were women. Mean myocardial fibrosis percentage on MRI was 10.7 ± 13.1% and mean ventricular thickness was18.7 ± 6.7 mm. Four patients had the following GLA gene mutations: c.967C&gt;A (p.Pro323Thr), not yet described in the literature; c.937G&gt;T (p.Asp313Tyr); and c.352C&gt;T (p.Arg118Cys). All patients had normal levels of lyso-Gb3 and non-ischemic myocardial fibrosis on magnetic resonance imaging; one patient had proteinuria and one patient had ventricular tachycardia. Conclusion: in this study, the frequency of mutation in the GLA gene in patients with HCM was 6.7%. A novel mutation in exon 6 of the GLA gene, c.967C&gt;A (p.Pro323Thr), was identified. Patients with HCM may have GLA mutations and FD should be ruled out. Plasma (lyso-Gb3) levels do not seem to be sufficient to attain a diagnosis and organ biopsy should be considered.
DOI: 10.6084/m9.figshare.8987843
title: Agrometeorological analysis of the soybean potentiality in an Amazonian environment

description: ABSTRACT The use of crop models that integrate soil, climate, cultivar and management information may broaden the understanding of the interactions between soybean cropping system and local climate variability. This study aimed to analyze the potentiality of soybean in an Amazonian production environment, as well as to determine an optimal sowing window via agrometeorological modeling. A crop model was programmed to simulate the soybean yield for 18 sowing dates, obtained along 33 years of climatic data, under attainable and potential conditions. The simulated potential of soybean yield ranged from 3,785 kg ha-1 to 5,114 kg ha-1, owing to the local energy availability, whereas the average attainable yield ranged from 557 kg ha-1 to 4,700 kg ha-1, mainly because of the soil moisture conditions. The smallest difference between the potential and attainable yields was observed in the sowing dates from 01-Jan to 15-Feb. For this window, the probability of obtaining yields above 3,500 kg ha-1 was higher than 90 %.
DOI: 10.6084/m9.figshare.8987837.v1
title: Seed physiological quality and harvest point of dovyalis fruits

description: ABSTRACT The main propagation mode of dovyalis is by seeds, what makes important to determine the ideal moment of fruit harvest. This study aimed to evaluate the physiological seed quality and harvest time of dovyalis fruits. To determine the fruit physical attributes, they were visually classified into five classes, according to the maturity stages, based on the external color of the exocarp: stage 1: green fruit; stage 2: yellowish-green; stage 3: yellowish-brown; stage 4: brown; stage 5: intense brown. The seed extraction to evaluate the physiological quality was based on the seed water content, germination percentage, germination speed index and determination of the dry biomass of the shoot, root and seedlings originated from these seeds. The experimental design was completely randomized, containing 25 seeds per treatment and 4 replicates. The fruit color during ripening varies from green to intense brown. The fruits reach their largest size and fresh biomass accumulation in the stage 4, with the brown exocarp color represented in the Munsell chart by 2.5YR 3/4. The removal of seeds for propagation should occur from the stage 2 (2.5GY 5/8), when the fruits have a yellowish-green color, although no statistical variation is observed in the number of seeds during maturation.
DOI: 10.6084/m9.figshare.8987843.v1
title: Agrometeorological analysis of the soybean potentiality in an Amazonian environment

description: ABSTRACT The use of crop models that integrate soil, climate, cultivar and management information may broaden the understanding of the interactions between soybean cropping system and local climate variability. This study aimed to analyze the potentiality of soybean in an Amazonian production environment, as well as to determine an optimal sowing window via agrometeorological modeling. A crop model was programmed to simulate the soybean yield for 18 sowing dates, obtained along 33 years of climatic data, under attainable and potential conditions. The simulated potential of soybean yield ranged from 3,785 kg ha-1 to 5,114 kg ha-1, owing to the local energy availability, whereas the average attainable yield ranged from 557 kg ha-1 to 4,700 kg ha-1, mainly because of the soil moisture conditions. The smallest difference between the potential and attainable yields was observed in the sowing dates from 01-Jan to 15-Feb. For this window, the probability of obtaining yields above 3,500 kg ha-1 was higher than 90 %.
DOI: 10.6084/m9.figshare.8987846.v1
title: Population and reproductive parameters of the red-tailed catfish, Phractocephalus hemioliopterus (Pimelodidae: Siluriformes), from the Xingu River, Brazil

description: ABSTRACT A field study of the sex ratio, length-weight ratio, body size at first gonadal maturation, and the breeding season of the red-tailed catfish, Phractocephalus hemioliopterus, was carried out in the region of the middle Xingu River in the eastern Amazon basin. The fish were sampled quarterly from September 2012 to January 2014 using gillnets and long lines. The sex ratio was balanced, and while males and females were similar in standard length, the females were heavier than the males, on average. The males presented negative allometric growth, and the females, isometric growth. The estimated size at first maturity was 77.8 cm. The variation in the gonadosomatic index and the greater frequencies of mature specimens indicated a reproductive peak at the beginning of the high-water (flood) period. As P. hemioliopterus is an important commercial fish, our findings will contribute to the future development of effective conservation and management strategies, such as minimum catch size and a close season that is appropriate for the region. These data will also provide important insights for the evaluation of potential impacts on the biology of this fish resulting from the construction of Belo Monte dam.
DOI: 10.6084/m9.figshare.8987840.v1
title: GLA Gene Mutation in Hypertrophic Cardiomyopathy with a New Variant Description: Is it Fabry's Disease?

description: Abstract Background: Fabry disease (FD) is an X-linked lysosomal storage disorder caused by mutations in the alpha galactosidase A gene (GLA) that lead to the enzymatic deficiency of alpha galactosidase (α-Gal A), resulting in the accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3), causing multiple organ dysfunctions. Objective: To perform GLA gene screening in a group of patients with echocardiographic diagnosis of hypertrophic cardiomyopathy (HCM). Methods: a cross-sectional study was conducted with HCM patients from a university hospital. Patients with coronary artery disease and valvulopathies were excluded. Mutation analysis of the GLA gene was performed. In male subjects, the analysis was performed after evidence of low α-Gal A activity. Results: 60 patients with echocardiographic diagnosis of HCM were included. Age ranged from 12 to 85 years and 60% were women. Mean myocardial fibrosis percentage on MRI was 10.7 ± 13.1% and mean ventricular thickness was18.7 ± 6.7 mm. Four patients had the following GLA gene mutations: c.967C&gt;A (p.Pro323Thr), not yet described in the literature; c.937G&gt;T (p.Asp313Tyr); and c.352C&gt;T (p.Arg118Cys). All patients had normal levels of lyso-Gb3 and non-ischemic myocardial fibrosis on magnetic resonance imaging; one patient had proteinuria and one patient had ventricular tachycardia. Conclusion: in this study, the frequency of mutation in the GLA gene in patients with HCM was 6.7%. A novel mutation in exon 6 of the GLA gene, c.967C&gt;A (p.Pro323Thr), was identified. Patients with HCM may have GLA mutations and FD should be ruled out. Plasma (lyso-Gb3) levels do not seem to be sufficient to attain a diagnosis and organ biopsy should be considered.
DOI: 10.6084/m9.figshare.8987828
title: Azospirillum brasilense inoculation methods in corn and sorghum

description: ABSTRACT The Azospirillum brasilense inoculation is usually carried out via seed. However, this bacterium presents passive penetration in the plant, what makes it possible to inoculate the crops using other methods. This study aimed to evaluate the influence of A. brasilense inoculation methods on the emergence and initial growth of corn and sorghum, under greenhouse conditions. A randomized block design, with four replications, in a 4 x 2 factorial scheme, was used. The first factor consisted of a control treatment (without inoculation) and three inoculation methods: via seed, via sowing furrow and via leaf spray. The second factor was composed by two hybrids of corn and two of sorghum. The application of A. brasilense to the seed can reduce the emergence percentage and the emergence speed index of corn seedlings, depending on the genotype, but its application does not influence the initial growth of the plants. The A. brasilense inoculation via seed and via sowing furrow can reduce the emergence and increase the mean emergence time of sorghum seedlings, depending on the genotype. The A. brasilense inoculation via seed results in an increase in the dry mass of the root system of sorghum plants.
DOI: 10.6084/m9.figshare.8987834
title: People, Manatees and the Aquatic Environment in Early Modern Americas: Confluence and Divergence in the Historical Relationships Between Humans and Animals

description: ABSTRACT The manatee (manati of the West Indies, also called manatim or iguaragua in colonial Brazil) was highly valued and presented a broad historical geographic distribution in coastal, transitional and fluvial areas of the tropical Atlantic Ocean. Documental and iconographic sources of the 16th and 17th centuries show us that it was used as food and for medicinal purposes, to manufacture tools or even as a pet. Moreover, its economic and symbolic value was equally relevant. Continued overexploitation led to the disappearance of many populations of manatees, which are currently at the brinck of extinction. We will explore, from the point of view of marine environmental history, the pragmatic and symbolic meaning of the manatee in close connection to the importance given to rivers and watercourses. We also expect to contribute to place manatees and their ecosystem at the center of the discussion about the environmental and sociocultural contexts and interactions of indigenous and colonial societies in the Americas of the modern period.
DOI: 10.6084/m9.figshare.8987825
title: Preliminary investigation of Terahertz spectroscopy to predict pork freshness non-destructively

description: Abstract Freshness, a very important criterion for pork quality control, is normally assessed by the index of K value. In this paper, Terahertz (THz) spectroscopy was employed to predict K value of pork nondestructively. The THz spectra (0.2~2.0THz) of 80 pork samples with different freshness in the attenuated total reflectance (ATR) mode were acquired. Simultaneously, their K values were determined by high performance liquid chromatography (HPLC). A back propagation artificial neural network (BP-ANN) prediction model of K value was established. The precision of BP-ANN was further improved after optimization by the algorithm of Adaptive boosting (AdaBoost), whose root mean square error of prediction (RMSEP) and correlation coefficient (RP) were 9.89% and 0.84 respectively in the prediction set, indicating that the non-linear models (BP-ANN and BP-AdaBoost) were superior to the linear principal component regression (PCR) model. The topological neural network architecture was much more suitable for analyzing complicated regression relationship between K value and THz spectra. It can be concluded that the THz spectral coupled with BP-AdaBoost algorithm is capable of predicting the pork K value.
DOI: 10.6084/m9.figshare.8987828.v1
title: Azospirillum brasilense inoculation methods in corn and sorghum

description: ABSTRACT The Azospirillum brasilense inoculation is usually carried out via seed. However, this bacterium presents passive penetration in the plant, what makes it possible to inoculate the crops using other methods. This study aimed to evaluate the influence of A. brasilense inoculation methods on the emergence and initial growth of corn and sorghum, under greenhouse conditions. A randomized block design, with four replications, in a 4 x 2 factorial scheme, was used. The first factor consisted of a control treatment (without inoculation) and three inoculation methods: via seed, via sowing furrow and via leaf spray. The second factor was composed by two hybrids of corn and two of sorghum. The application of A. brasilense to the seed can reduce the emergence percentage and the emergence speed index of corn seedlings, depending on the genotype, but its application does not influence the initial growth of the plants. The A. brasilense inoculation via seed and via sowing furrow can reduce the emergence and increase the mean emergence time of sorghum seedlings, depending on the genotype. The A. brasilense inoculation via seed results in an increase in the dry mass of the root system of sorghum plants.
DOI: 10.6084/m9.figshare.8987831
title: Population medical genetics: translating science to the community

description: Abstract Rare genetic disorders are currently in the spotlight due to the elevated number of different conditions and significant total number of affected patients. The study of these disorders is extremely helpful for the elucidation of physiological processes related with complex disorders. Isolated populations are instrumental for the study of genetic disorders, considering their homogeneity and high proportion of affected patients in a small geographic area. These favorable conditions lead to the creation of a new discipline, known as “population medical genetics”, which integrates medical genetics, population genetics, epidemiological genetics and community genetics. In order to develop practical activities in this new discipline, the National Institute of Population Medical Genetics (INaGeMP) was created in 2008 in Brazil. INaGeMP has developed several tools and funded numerous research activities. In this review, we highlight three successful projects developed in the first 10 years of INaGeMP activities (2008-2018): a newborn screening pilot study for MPS VI in Northeast Brazil, the study of Machado-Joseph disease in Brazilian families with Azorian ancestry, and the high twinning rate in a small town in southern Brazil. The results of these projects in terms of scientific output and contributions to the affected communities highlight the success and importance of INaGeMP.
DOI: 10.6084/m9.figshare.8987825.v1
title: Preliminary investigation of Terahertz spectroscopy to predict pork freshness non-destructively

description: Abstract Freshness, a very important criterion for pork quality control, is normally assessed by the index of K value. In this paper, Terahertz (THz) spectroscopy was employed to predict K value of pork nondestructively. The THz spectra (0.2~2.0THz) of 80 pork samples with different freshness in the attenuated total reflectance (ATR) mode were acquired. Simultaneously, their K values were determined by high performance liquid chromatography (HPLC). A back propagation artificial neural network (BP-ANN) prediction model of K value was established. The precision of BP-ANN was further improved after optimization by the algorithm of Adaptive boosting (AdaBoost), whose root mean square error of prediction (RMSEP) and correlation coefficient (RP) were 9.89% and 0.84 respectively in the prediction set, indicating that the non-linear models (BP-ANN and BP-AdaBoost) were superior to the linear principal component regression (PCR) model. The topological neural network architecture was much more suitable for analyzing complicated regression relationship between K value and THz spectra. It can be concluded that the THz spectral coupled with BP-AdaBoost algorithm is capable of predicting the pork K value.