cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j01v5d98
title: CIL:39245, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A view of a part of the oral apparatus of Conchophthirus which reveals details of cortical structure. This includes one oral membranelle and three vestibular kinities as well as cytoplasm and cortical structure. The negative magnification is 6,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0513xjt
title: CIL:39242, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The macronucleus, one micronucleus, and the cytoplasm of Conchophthirus. The negative magnification is 6,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j00c4td2
title: CIL:39306, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: This EM features the infraciliary lattice which forms a continuous network at the level of the proximal ends of basal bodies where the kinetodesmal fibers are connected to the posterior basal bodies of a pair. This system is separated by a small gap from the more superficially located striated bands. The gap has no apparent fibers connecting these two systems. This network forms a highly porous boundary between the ectoplasm and the endoplasm. Electron dense posts are located at each branching site of this network. It is now known that this network is composed of centrin, a contractile protein also found in centrosomes of higher animals including man. TEM taken on 12/18/68 by R. Allen with Philips 300. Neg. 16,000X. Published in J. Cell Biol. 49:1-20, 1971.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: transmission electron microscopy (TEM)
Source of contrast: electron density; stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j02v2dr8
title: CIL:39254, Mus musculus, myoblast. In Cell Image Library

description: C2C12 cells were transfected with GFP-PCNA and 24 hr later pulse labeled with BrdU, fixed, and stained with anti-BrdU antibodies. optical sections acquired with a Leica TCS confocal microscope. Shown are stacks of 4-5 midplane sections.

See: Fig 2B in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
description: Mouse myoblast cells were transfected with GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, pulsed with BrdU, fixed, and immunostained with anti-BrdU. The image shows t different typical S-phase patterns displaying GFP-PCNA (left, green), BrdU (red, center) and the merged images (right). The dominant yellow coloration in the merges shows the strong localization of PCNA at replication foci.
 Other images in this group show the co-localization of enzymes associated with DNA replication at foci and the dynamic changes in the GFP-PCNA signal in individual foci over time.
description: Preparation: formaldehyde fixed tissue; living tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT); charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Fluorescein (FITC)
Processing history: montage
Data qualification: Processed
DOI: 10.6075/j0833rbp
title: CIL:39250, Didinium nasutum, Paramecium sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Didinium captures Paramecium. It is not unusual for an undersized Didinium to attempt to ingest a much larger Paramecium. In this case, the organisms were preserved before the outcome could be determined. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 440X. Further details are available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270.
description: Preparation: chemically fixed tissue; lyophilized specimen; Parducz fixative; Au/Pd metal sputter-coat
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: illumination by electrons; secondary_electron imaging
Parameter imaged: illumination by electrons; secondary electron generation; secondary_electron imaging
Source of contrast: detection of electrons
Visualization methods: shadowing and plating
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j00c4v3f
title: CIL:39238, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: A transverse section of the cortex of Conchophthirus curtus reveals details of cortical structure. This includes the nature of the cell membrane and the alveolar membranes that reside immediately below the surface; fibrillar and microtubular structures that support this ciliated cell, and in the case of Conchophthirus, the cortical mitochondria that may represent a single mitochondrion that has finger-like extensions that lie immediately between the rows of basal bodies and are independent of more typical vesicular mitochondria that reside in the inner cytoplasm. Details of the basal bodies are revealed. The negative magnification is 15,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
DOI: 10.6075/j0c53k6f
title: CIL:39237, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
description: Vestibular kinities and the cortical architecture adjacent to the oral apparatus of Conchophthirus curtus. The negative magnification is 15,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501 and Antipa, G. A. and Small, E. B. 1971. A redescription of Conchophthirus curtus Engelmann, 1862 (Protozoa, Ciliatea). J.¬†Protozool. 18:491-503.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j07d2sv7
title: CIL:39236, Mus musculus, fibroblast. In Cell Image Library

description: Shown are 0.5 micrometer thick confocal image of midplane sections of synchronized 3T3 fibroblasts pulsed for 2 min with CldU and labeled with FITC (green) followed by a variable chase time, and a second pulse with IdU and subsequently labeled with Cy-5 (red). With simultaneous pulses, the green and red signals overlap (A), but with longer chases, the two sets of replication sites become increasingly separate (B-F).
description: Cells were fixed with 70% ethanol at -20C, DNA denatured with 4N HCl for 30 min at room temperature, and processed for immunofluorescence using anti-CldU antibody and an appropriate FITC labeled secondary antibody. IuD was detected with a Cy-5 conjugated secondary antibody. Cells were examined in a MRC-1024 confocal microscope mounted on a Nikon Optphot 2 microscope with 60x 1.4 NA objective lens.

See: Fig 3 in, Ma, H et al. 1998 Spatial and temporal dynamics of DNA replication sites in mammalian cells. J Cell Biol 143:1415-1425.
description: Preparation: ethanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Fluorescein (FITC); TexasRed
Processing history: montage
Data qualification: Processed
DOI: 10.6075/j0gf0s51
title: CIL:39235, Macropus sp. MG002, kidney cell. In Cell Image Library

description: Shown is a chromosome spread of a PtK1 cell pulsed with BrdU in early S phase, then treated with colcemid to arrest cells at metaphase. Spreads were immuno-labeled with FITC-conjugated anti-BrdU antibodies and counterstained with propidium iodide (red). Replication is confined to discrete sites of replication, some of which appear to occur in bands (arrows).
description: Preparation: ethanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: photomultiplier tube (PMT)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Fluorescein (FITC); TexasRed
Processing history: montage
Data qualification: Processed
description: Metaphase-arrested PtK1 Cells were swollen, pre-fixed in methanol-acetic acid, dropped onto cover glasses, incubated with H2O to promote spreading, and air dried. After hardening by a 4 hr incubation at 65C samples were fixed with 70% ethanol at -20C. Spreads were denatured with 4N HCl for 30 min at room temperature, and processed for immunofluorescence using an anti-BrdU antibody and FITC-conjugated secondary antibody. Cells were examined in a MRC-1024 confocal microscope mounted on a Nikon Optphot 2 microscope with 60x 1.4 NA objective lens.

See: Fig 6 in, Ma, H et al. 1998 Spatial and temporal dynamics of DNA replication sites in mammalian cells. J Cell Biol 143:1415-1425.
DOI: 10.6075/j08k78d4
title: CIL:39232, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A glancing view of the cortex of Conchophthirus reveals details of cortical structure. A near serial section with CIL:39239. This includes the nature of the cell membrane and the alveolar membranes that reside immediately below the surface; fibrillar and microtubular structures that support this ciliated cell, and in the case of Conchophthirus, the cortical mitochondria that may represent a single mitochondrion that has finger-like extensions that lie immediately between the rows of basal bodies and are independent of more typical vesicular mitochondria that reside in the inner cytoplasm. The negative magnification is 14,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0tt4q9d
title: CIL:39230, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A glancing view of the thigmotactic ciliature of Conchophthirus reveals details of cortical structure. CIL:39231 is a near serial section. Both exhibit the characteristic zig-zag kinetal arrangement of the diplokinities found in the thigmotactic ciliature of conchophthirids. This includes the nature of the cell membrane and the alveolar membranes that reside immediately below the surface; fibrillar and microtubular structures that support this ciliated cell, and in the case of Conchophthirus, the cortical mitochondria that may represent a single mitochondrion that has finger-like extensions that lie immediately between the rows of basal bodies and are independent of more typical vesicular mitochondria that reside in the inner cytoplasm. The negative magnification is 13,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0dv1j7x
title: CIL:39226, Psophus stridulus, spermatocyte. In Cell Image Library

description: The movie was originally recorded on 35mm film, and later transferred to CD format. The text at the beginning of the movie translates approximately as follows:

Meiosis division I
Metaphase ( ca. 9-10 hr)
and
Anaphase (ca. 1-2 hr) 
-----------------------
 Based on the duration, it appears the movie represents an increased speed of ca. 50x.

See also:
Michel, K. (1941): Die Darstellung von Chromosomen mittels des Phasenkontrastverfahrens. Die Naturwissenschaften 29, 61-62 
Michel, K. (1958): Die Reifeteilungen (Meiose) bei der Spermatogenese der Schnarrheuschrecke (Psophus stridulus l.) Institut für den Wissenschaftlichen Film, Göttingen
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: film
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: Time lapse movie of meiotic metaphase and anaphase in spermatocytes of the Locust Psophus stridulus. Two sequences are shown, each continuing through telophase to the start of cytokinesis. In addition to the squat meiotic chromosomes, the spindles are clearly visible.
 This is a historic movie made in the early 40s by Kurt Michel of the Zeiss company in Jena using Zernike's newly invented phase contrast microscope. 
 Despite its early date, the movie and the others in the group remain some of the clearest examples of chromosome behavior during meiosis.
DOI: 10.6075/j0k073mp
title: CIL:39225, Psophus stridulus, spermatocyte. In Cell Image Library

description: Time lapse movie of the prophase stage during meiosis I in spermatocytes of the Locust Psophus stridulus.
 This is a historic movie made in the early 40s by Kurt Michel of the Zeiss company in Jena using Zernike's newly invented phase contrast microscope. Several sequences are shown, some proceeding to metaphase. 
 Despite its early date, the movie and the others in the group remain some of the clearest examples of chromosome behavior during meiosis.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: film
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: The movie was originally recorded on 35mm film, and later transferred to CD format. The text at the beginning of the movie translates approximately as follows:
From the microlaboratory of the Carl Zeiss Company, Jena
Director: Dr. K. Michel
Meiosis in spermatogenesis in the locust Psophus stridulus L
Scientific direction, production and filming by Dr. K. Michel
Recorded with the phase contrast procedure of Zernike.
Meiosis division I
Prophase
(Duration ca. 2 hr) 

IWF is the logo of The Institut fur den wissenscaftliche Film (Institute for scientific film located in Goettingen)
-----------------------
 Based on the 2 hr real time duration, it appears the movie represents an increased speed of ca. 50x.

See also:
Michel, K. (1941): Die Darstellung von Chromosomen mittels des Phasenkontrastverfahrens. Die Naturwissenschaften 29, 61-62 
Michel, K. (1958): Die Reifeteilungen (Meiose) bei der Spermatogenese der Schnarrheuschrecke (Psophus stridulus l.) Institut für den Wissenschaftlichen Film, Göttingen
DOI: 10.6075/j0gb22q7
title: CIL:39213, Homo sapiens, cardiomyocytes. In Cell Image Library

description: Fluorescent micrograph showing human induced pluripotent stem (iPS) cell-derived cardiomyocytes (CDI iCell) treated with 2 microM Erythromycin for 4 hours and stained for cell membrane permeabilization (Propidium Iodide, red), calcium mobilization (Fluo-4 AM, green) and Hoechst 33342 to visualize nuclei (blue). Images of live cells were taken with GE IN Cell Analyzer 1000 (20x objective).
description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Hoechst 33342; propidium iodide; Fluo-4 AM
DOI: 10.6075/j0vq3191
title: CIL:39209, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A cross section of the cell (ventral side up) through the buccal cavity showing basal bodies of the 3 membranelles (1, 2 and 3) lying along the indented dorsal wall. A short segment of the oral ribs separates the membranelles from the undulating membrane that lies at the margin of the right wall. Basal body/cilium complexes of a kinety are found at the margin of the left wall. The left wall is covered by ER on its cytosolic side. TEM taken on 8/25/67 by R. Allen with Philips 200 operating at 60kV. Neg. 4,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).