DOI: 10.22002/701s6-vf312
title: Coxiella burnetii_wt

description: Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type  cells day-5 culture.
description: <strong>Tilt Series Date:</strong> 2016-09-09</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab</p>
  dga2016-09-09-17_slicer17071.jpg: Arrays(?) in the periplasmic space
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-09-17/rawdata/</p>Coxiella_Wt_D50022.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
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        Coxiella_Wt_D50022_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
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        dga2016-09-09-17_slicer17215.png,  Very last moment of constriction. Sharp edges connecting!, 541.6 kB  
        
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        Coxiella_Wt_D50022.ali,  577.3 MB  
        
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        Coxiella_Wt_D50022.tlt,  648.0 B  
        
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        T4SS.mod,  987.0 B  
        
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        T4SS_all.mod,  1.0 kB  
        
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        T4SS_wifi.mod,  1.0 kB  
        
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        T4SS_wifi_2k.mod,  1.0 kB  
        
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