DOI: 10.5281/zenodo.2228371
title: Quantitative data on expression of eve, gt and hb in early Drosophila embryo

description: This dataset is supplementary to the following manuscript:

Svetlana Surkova, Alena Sokolkova, Konstantin Kozlov, Sergey V. Nuzhdin and Maria Samsonova (2019). Quantitative analysis reveals genotype- and domain- specific differences between mRNA and protein expression of segmentation genes in <em>Drosophila</em>. <strong><em>Developmental Biology</em></strong>,  doi: 10.1016/j.ydbio.2019.01.006.

The archive contains recently obtained quantitative data on expression of <em>eve, gt</em> and <em>hb</em> in <em>Drosophila</em> early embryo at the level of mRNA and protein. It also includes integrated expression profiles of these genes, combined with the prior datasets (Surkova et al., 2008, Surkova et al., 2013).

Embryos are classified into 8 time classes within cleavage cycle 14A. Genotype is specified as “wt” (wild type), “het” (<em>Kr+/Kr-</em> heterozygotes) and "mut" (<em>Kr</em> null mutants).

Stained channels: EHG (<em>eve</em> mRNA, <em>hb</em> mRNA, <em>gt</em> mRNA); GGE (<em>gt</em> mRNA, Gt protein, <em>eve</em> mRNA); GE (<em>gt </em>mRNA, <em>eve</em> mRNA); ghe (<em>gt</em> mRNA, Hb protein, <em>eve</em> mRNA); HHE (Hb protein, <em>hb</em> mRNA, <em>eve</em> mRNA); GHH (<em>gt</em> mRNA, Hb protein, <em>hb</em> mRNA).

<strong>The data are arranged in three folders:</strong>

<strong>1. QData_norm</strong>

Includes coordinates and fluorescence intensities of segmented nuclei for each individual embryo after subtraction of non-specific background signal.

<em>Files are arranged according to time class (T1-T8) and named according to the following scheme:</em>

&lt;stained channel&gt;&lt;number&gt;_norm.txt

<em>Structure of data file:</em>

<strong>A-P-coord | D-V-coord | channel 1 | channel 2 | channel 3 </strong>

<strong>2. QData_registered</strong>

Registered one-dimensional gene expression profiles.

<em>Files are arranged according to time class (T1-T8) and named according to the following scheme:</em>

&lt;stained channel&gt;&lt;number&gt;_reg.txt

<em>Structure of data file:</em>

<strong>Number | A-P-coord | channel 3 | channel 2 | channel 1</strong>

<strong>3. Integrated_data</strong>

Integrated one-dimensional profiles of <em>eve, hb</em> and <em>gt</em> expression at mRNA and protein levels for wild type embryos, <em>Kr</em> mutants and <em>Kr+/Kr</em>- heterozygotes.

<em>eve </em>and <em>gt</em> profiles were normalized on stripe 1 and second anterior domain respectively (see "Materials and Methods)".

<em>Files are named according to the following scheme:</em>

<strong>&lt;gene&gt;&lt;genotype&gt;&lt;gene product&gt;&lt;time class&gt;_100</strong>

<em>Structure of data file:</em>

<strong>Number | A-P<em> </em>coord of an averaged nucleus | averaged intensity</strong>

<em>hb profiles are named according to the following scheme:</em>

<strong>&lt;gene&gt;&lt;genotype&gt;&lt;gene product&gt;&lt;time class&gt;</strong>

<em>Structure of data file:</em>

<strong>Number | A-P<em> </em>coord of an averaged nucleus | averaged intensity | standard deviation</strong>

<strong>References</strong>


	Surkova S., Kosman D., Kozlov K., Myasnikova E., Samsonova A.A., Spirov A., Vanario-Alonso C.E., Samsonova M., Reinitz J. (2008). Characterization of the <em>Drosophila</em> segment determination morphome. Developmental biology 313(2):844-862.
	Surkova S., Golubkova E., Manu, Panok L., Mamon L., Reinitz J., Samsonova M. (2013) Quantitative dynamics and increased variability of segmentation gene expression in the <em>Drosophila Kruppel</em> and <em>knirps</em> mutants (2013). Dev Biol. 376: 99-112. doi: 10.1016/j.ydbio.2013.01.008.