DOI: 10.5061/dryad.5dv41nsfm
title: Diplazium zangnanense, a new species of the twinsorus-fern genus Diplazium (Athyriaceae) from Xizang Province, China

description: Diplazium zangnanense, a new species of Diplazium Sw. from Medog county,
 Xizang Autonomous Region, China, is described and illustrated.
 Morphological comparisons between this new species and similar ones (e.g.,
 D. kawakamii, D. medogense, D. muricatum, D. multicaudatum, and D.
 spectabile, etc.) are provided. Molecular evidence shows that the new
 species is closely related to D. multicaudatum. The most distinctive
 characters of D. zangnanense are its decumbent to erect rhizomes,
 bicolorous scales with toothed margin on the stems, and oblong sori.
description: We combined all six chloroplast markers(<em>atpA,atpB, matK
 , rps4-trnS IGS, trnL-F, matK </em>) in one dataset and analyzed
 them using the maximum likelihood (ML) and Bayesian inference (BI)
 methods. For ML and BI analyses, we used PartitionFinder v.2.1.1 (Lanfear
 et al., 2012) to determine the appropriate DNA substitution model
 according to the corrected Akaike information criterion (AICc). The ML
 analysis was conducted using IQ-Tree v.1.6 (Nguyen et al., 2014), with
 bootstrap values (BS) calculated based on 1,000 bootstrap replications.
 The BI analysis was performed in MrBayes v.3.2.6 (Ronquist et al., 2012)
 based on the same partitioning schemes and substitution model as indicated
 in the ML analysis. Four Markov Chain Monte Carlo (MCMC) chains were run,
 and every chain was generated for 3,000,000 generations with one cold
 chain and three hot chains. We sampled the cold chain every 1,000
 generations. We combined the independent runs to obtain majority-rule
 consensus trees and to calculate posterior probabilities (PP).
description: # *Diplazium zangnanense*, a new species of the twinsorus-fern genus
 *Diplazium* (Athyriaceae) from Xizang Province, China
 [https://doi.org/10.5061/dryad.5dv41nsfm](https://doi.org/10.5061/dryad.5dv41nsfm) ## Description of the data and file structure We have submitted our raw alignment data(**LH_Diplazium_lmjnew_corrected.fas**),GenBank number and voucher information(**Table_S1.docx**),raw trimmed phylogenetic trees based on Bayesian inference (BI) methods methods(**LH_Diplazium_BI.con.tre**),raw trimmed phylogenetic trees based on maximum likelihood (ML) methods (**LH_Diplazium_ML.contree**). **Data Files (Dryad)** **LH_Diplazium_lmjnew_corrected.fas** * The dataset includes chloroplast sequences of other species that are closely related to the new species,including matK, rbcL, rps4, trnL-F, rps4-trnS IGS, atpA, and atpB. **LH_Diplazium_BI.con.tre** * Used alignment data(**LH_Diplazium_lmjnew_corrected.fas**) constructed the phylogenetic tree. Based on Bayesian inference (BI) methods. **LH_Diplazium_ML.contree** * Used alignment data(**LH_Diplazium_lmjnew_corrected.fas**) constructed the phylogenetic tree. Based on maximum likelihood (ML) methods. **Supplemental Files (Zenodo)** **Table_S1.docx** * *Species*: Currently valid scientific name. * *Voucher information(herbarium)*: The provenance of the sequence or the collection information of the specimen. * *GenBank accession numbers* : GenBank accession numbers of the sequences, reader can use these numbers to download sequences easily from NCBI. ## Code/software Use Genious, MEGA can open and edit **LH_Diplazium_lmjnew_corrected.fas**, you can use some text readers to read it, but please don't use text reader to edit alignment file it may cause some error. **Table_S1.docx** can use Word to read. **LH_Diplazium_BI.con.tre** and **LH_Diplazium_ML.contree** can use Figtree software to read, and also can use some online website to read, like:itol([https://itol.embl.de/](https://itol.embl.de/)) ## Access information Other publicly accessible locations of the data: * NA Data was derived from the following sources: * NCBI [National Center for Biotechnology Information (nih.gov)](https://www.ncbi.nlm.nih.gov/)