cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.vw3z6b
title: Occurrence Download

description: A dataset containing 1008 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "Country is Peru",
 "PublishingOrg is one of (90fd6680-349f-11d8-aa2d-b8a03c50a862, 061b4f20-f241-11da-a328-b8a03c50a862)",
 "TaxonKey is Gentianella Moench"
 ]
}

 The dataset includes 1008 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019673-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.st54nj
title: Occurrence Download

description: A dataset containing 501299 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Bromus hordeaceus L."
 ]
}

 The dataset includes 501299 records from 3251 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019634-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/r6ef0-dff11
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-22/3dimage_6811/</p>034.rec,  Reconstruction (Pixel Size 1.922 nm), 102.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-22/3dimage_6811/034.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ef4hq-jfx54
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-18/3dimage_6807/</p>030.rec,  Reconstruction (Pixel Size 1.922 nm), 85.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-18/3dimage_6807/030.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/d5g67-qgy90
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-1/3dimage_6790/</p>002.rec,  Reconstruction (Pixel Size 1.922 nm), 226.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-1/3dimage_6790/002.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2swqt-jnn60
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-13/3dimage_6802/</p>020.rec,  Reconstruction (Pixel Size 1.922 nm), 141.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-13/3dimage_6802/020.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rydk6-eng50
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-29/3dimage_6821/</p>09jul11a_atlasming1_020.rec,  Reconstruction (Pixel Size 1.922 nm), 141.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-29/3dimage_6821/09jul11a_atlasming1_020.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1eq9g-1pe82
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-25/3dimage_6814/</p>039.rec,  Reconstruction (Pixel Size 1.922 nm), 68.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-25/3dimage_6814/039.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0encg-1j603
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-9/3dimage_6798/</p>016.rec,  Reconstruction (Pixel Size 1.922 nm), 99.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-9/3dimage_6798/016.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a3zpm4
title: Occurrence Download

description: A dataset containing 144 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Haplopappus chrysanthemifolius"
 ]
}

 The dataset includes 144 records from 31 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006615-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/1mk2a-b4095
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-5/3dimage_6794/</p>011.rec,  Reconstruction (Pixel Size 1.922 nm), 6.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-5/3dimage_6794/011.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cavc8-4yd98
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-17/3dimage_6806/</p>029.rec,  Reconstruction (Pixel Size 1.922 nm), 145.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-17/3dimage_6806/029.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hvt24-j2c28
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-16/3dimage_6805/</p>026.rec,  Reconstruction (Pixel Size 1.922 nm), 2.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-16/3dimage_6805/026.rec"
        > <i class="download icon"></i> Download </a></p>