DOI: 10.22002/vzat6-94t87
title: Caulobacter crescentus

description: wild type CB15N. 4 of 8, some warping at lower edge of hole. stalked cell; very dense 'medusa-head' feature; amorphous blob; thick paired filament at upper end, fainter similar filament near lower end; strange tubular structure next to cell on concave side
description: <strong>Tilt Series Date:</strong> 2006-06-22</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; 5 days growth; see DPD notebook Jun8-12, 2006</p>
<strong>Sample Preparation:</strong> box 694, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006</p>
  SAVE_filpair_xy_01.jpg: file associated to 3D Image #308 SAVE_filpair_xy_02.jpg: file associated to 3D Image #308 SAVE_filpair_yz_01.jpg: file associated to 3D Image #308 SAVE_filposs_xy_01.jpg: file associated to 3D Image #308 SAVE_membrane_xz_01.jpg: file associated to 3D Image #308 SAVE_membrane_yz_01.jpg: file associated to 3D Image #308 pdd2006-06-22-4_slicer10043.jpg: Caulobacter crescentus .  unknown pdd2006-06-22-4_slicer10044.jpg: Caulobacter crescentus .  periplasmic vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-4/rawdata/</p>06Jun22t04.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-4/rawdata/06Jun22t04.mrc"
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        06Jun22t04bn.rec,  Reconstruction (Pixel Size 2.4 nm), 164.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-4/3dimage_307/06Jun22t04bn.rec"
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        06Jun22t04bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
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        06Jun22t04_13kx.dm3,  * associated to rawdata, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-4/file_698/06Jun22t04_13kx.dm3"
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        06Jun22t04_18kx.dm3,  * associated to rawdata, 8.6 MB  
        
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        SAVE_filpair_xy_01.tif,  file associated to 3D Image #308, 3.0 MB  
        
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        SAVE_filpair_xy_02.tif,  file associated to 3D Image #308, 5.9 MB  
        
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        SAVE_filpair_yz_01.tif,  file associated to 3D Image #308, 5.7 MB  
        
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        SAVE_filposs_xy_01.tif,  file associated to 3D Image #308, 5.9 MB  
        
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        SAVE_membrane_xz_01.tif,  file associated to 3D Image #308, 5.5 MB  
        
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        SAVE_membrane_yz_01.tif,  file associated to 3D Image #308, 5.3 MB  
        
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        06Jun22t04b.mov,  file associated to 3D Image #308, 314.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-4/file_712/06Jun22t04b.mov"
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        Jun22t04sm.mov,  file associated to 3D Image #308, 19.9 MB  
        
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