cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/evl-h92-8zz
title: Dasypolia (Dasypolia) templi Thunberg, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/z1s-sx1-b03
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/6mr-z1d-9pm
title: Mythimna (Mythimna) impura Hübner, 1808

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/8jf-caw-z7n
title: Lycaena phlaeas (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/e7p-ejh-yd8
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/36k-7j6-f31
title: Rhizedra lutosa Hübner, 1803

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/1yr-tz6-sq9
title: Agriphila tristellus [Denis & Schiffermüller], 1775

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/kxh-bty-dnv
title: Acronicta (Subacronicta) megacephala ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/ltg-zqk-89s
title: Lithophane (Lithophane) consocia Borkhausen, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/mgh-crw-526
title: Lasionycta Aurivillius, 1892

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/0gm-eab-j01
title: Sphrageidus similis (Fuessly, 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/r4p-t27-d9l
title: Ochropacha duplaris (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/5za-k0r-khx
title: Ochropacha duplaris (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/sxj-gy2-e0b
title: Lithophane (Lithophane) consocia Borkhausen, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/7cd-xza-hsg
title: Drepana falcataria (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d18-vcl-f91
title: Dioryctria abietella ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/081-fv2-y46
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/6s9-xk2-t0p
title: Mythimna (Mythimna) impura Hübner, 1808

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/267-laj-t12
title: Lithophane (Lithophane) consocia Borkhausen, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/xw6-7fj-ag5
title: Acronicta (Subacronicta) megacephala ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/gw5-k8w-cbs
title: Orgyia (Thylacigyna) antiquoides (Hübner, 1822)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/v68-4gp-a6a
title: Ochropacha duplaris (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/qkr-nsf-6q0
title: Boloria Moore, 1900

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/vm8-gq7-m10
title: Fabriciana adippe ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/1zd-yzn-gv1
title: Drepana falcataria (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/j2f-67p-ebl
title: Apamea (Apamea) sublustris (Esper)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d05-h4s-3b8
title: Diachrysia chrysitis (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d0e-4d2-b2m
title: Ammoconia caecimacula [Denis & Schiffermüller], 1775

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/rtf-2j2-e3m
title: Dasypolia (Dasypolia) templi Thunberg, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/ehy-cpj-jt3
title: Rhizedra lutosa Hübner, 1803

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/j44-jy5-20q
title: Eudonia mercurella Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/e64-maa-gl7
title: Lithophane (Lithophane) consocia Borkhausen, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/kwl-aq8-fcn
title: Rhizedra lutosa Hübner, 1803

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/f0x-gvf-hh8
title: Acronicta (Subacronicta) megacephala ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/r9a-1te-7yz
title: Cyaniris semiargus (von Rottemburg, 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/01r-014-z9a
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/m89-jzm-7az
title: Anarta (Calocestra) melanopa Thunberg, 1791

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/qn5-rxw-9t4
title: Mythimna (Mythimna) impura Hübner, 1808

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/sx2-wy0-lye
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/c54-lc5-0sj
title: Dasypolia (Dasypolia) templi Thunberg, 1792

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/yna-ec0-5tl
title: Eudonia mercurella Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/f08-bnb-09x
title: Eudonia mercurella Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/sx0-k4s-y0s
title: Autographa Hübner, 1821

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/269-jc3-cwm
title: Eudonia mercurella Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/4hd-48s-q6d
title: Anarta (Calocestra) melanopa Thunberg, 1791

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/hn5-7yt-l2v
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/r4x-wqt-q5p
title: Anarta (Calocestra) melanopa Thunberg, 1791

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/az2-svf-ybq
title: Polymixis (Polymixis) polymita Linnaeus, 1761

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/m5k-c5s-r2q
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/2ve-53t-fqx
title: Drepana falcataria (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/mem-yel-ndm
title: Anarta (Calocestra) melanopa Thunberg, 1791

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/4pa-a7y-l6y
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/b34-0xb-e97
title: Agrochola (Anchoscelis) Guenée, 1839

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/fe4-vs1-zfc
title: Leptidea sinapis (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/qkl-0th-0y1
title: Cucullia umbratica (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/71m-x0p-gpq
title: Cyaniris semiargus (von Rottemburg, 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/cfc-h3t-q87
title: Protolampra sobrina Duponchel, 1843

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/9rh-ja2-bvd
title: Agriphila tristellus [Denis & Schiffermüller], 1775

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/yxk-t1v-hvy
title: Gonepteryx rhamni (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/q1d-33a-nn4
title: Gonepteryx rhamni (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/36a-hrn-d39
title: Apamea (Apamea) remissa (Hübner)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/8n3-xm7-j8m
title: Oeneis jutta Hübner, 1806

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/n20-da7-w81
title: Gonepteryx rhamni (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/sze-mec-cnb
title: Amphipoea fucosa Freyer, 1830

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/7r8-yew-vlf
title: Boloria selene ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/lpt-z2c-t7t
title: Amphipoea fucosa Freyer, 1830

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/hg1-hhg-am3
title: Autographa Hübner, 1821

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/v3d-1jr-pqy
title: Athetis (Athetis) gluteosa (Treitschke, 1835)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/ef0-y6k-bld
title: Lycaena Fabricius, 1807

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/ytv-fkh-yr2
title: Xestia (Megasema) triangulum Hufnagel, 1766

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.15482/usda.adc/30094714
title: Data from: Contemporary United States PRRSV 1-4-4 L1C.5 isolate causes severe disease comparable to historic highly pathogenic PRRSV

description: This online resource provides source data collected to generate plots and statistics in the work, "Contemporary United States PRRSV 1-4-4 L1C.5 isolate causes severe disease comparable to historic highly pathogenic PRRSV", by Wiarda et al. Weaned pigs were inoculated intranasally with mock inoculum or one of three porcine reproductive and respiratory syndrome viruses (PRRSVs), including a recombinant of the highly pathogenic PRRSV JXwn06 that emerged in Asia in 2006, a 2020 isolate of PRRSV 1-4-4 L1C.5 that has been shown to cause severe morbidity and mortality compared to other contemporary 1-4-4 strains, and moderately pathogenic PRRSV MN184 that emerged in the United States in the early 2000s. Pigs were monitored for signs of disease, and serum samples were collected throughout the study. Animals were necropsied at 2, 6, and 10 days post-infection, or as needed due to development of severe disease. Results demonstrated both PRRSV JXwn06 and the 1-4-4 L1C.5 isolate caused extreme morbidity and mortality that shared very similar disease dynamics. Animal meta data along with data collected for parameters of clinical disease, tissue viral loads, cytokine concentrations, antibody concentrations, gross pathology, and microscopic pathology are available here.
DOI: 10.15482/usda.adc/30094714.v1
title: Data from: Contemporary United States PRRSV 1-4-4 L1C.5 isolate causes severe disease comparable to historic highly pathogenic PRRSV

description: This online resource provides source data collected to generate plots and statistics in the work, "Contemporary United States PRRSV 1-4-4 L1C.5 isolate causes severe disease comparable to historic highly pathogenic PRRSV", by Wiarda et al. Weaned pigs were inoculated intranasally with mock inoculum or one of three porcine reproductive and respiratory syndrome viruses (PRRSVs), including a recombinant of the highly pathogenic PRRSV JXwn06 that emerged in Asia in 2006, a 2020 isolate of PRRSV 1-4-4 L1C.5 that has been shown to cause severe morbidity and mortality compared to other contemporary 1-4-4 strains, and moderately pathogenic PRRSV MN184 that emerged in the United States in the early 2000s. Pigs were monitored for signs of disease, and serum samples were collected throughout the study. Animals were necropsied at 2, 6, and 10 days post-infection, or as needed due to development of severe disease. Results demonstrated both PRRSV JXwn06 and the 1-4-4 L1C.5 isolate caused extreme morbidity and mortality that shared very similar disease dynamics. Animal meta data along with data collected for parameters of clinical disease, tissue viral loads, cytokine concentrations, antibody concentrations, gross pathology, and microscopic pathology are available here.
DOI: 10.3535/hs2-1tp-vqt
title: Hypochalcia ahenella [Denis & Schiffermüller], 1775

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/pb5-aaf-10p
title: Xestia (Megasema) ashworthii Doubleday, 1855

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/6zv-t3c-4k8
title: Cyaniris semiargus (von Rottemburg, 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/35n-kzq-c4a
title: Hydraecia micacea Esper, 1789

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/cpe-3c9-605
title: Hydraecia micacea Esper, 1789

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/kp6-je0-s2v
title: Acronicta (Subacronicta) megacephala ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/lwc-9ch-vgv
title: Glaucopsyche alexis (Poda, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/mc6-nm2-ev7
title: Acronicta (Subacronicta) megacephala ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/1bp-a2y-1s6
title: Agriphila tristellus [Denis & Schiffermüller], 1775

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/vsc-6f4-5vy
title: Apamea (Apamea) remissa (Hübner)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/361-wpz-qd6
title: Apamea (Apamea) remissa (Hübner)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/d69-tyc-63b
title: Gonepteryx rhamni (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/8de-mra-6bj
title: Autographa Hübner, 1821

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/7ns-40p-ly4
title: Glaucopsyche alexis (Poda, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/byg-2hx-9tb
title: Diarsia dahlii Hübner, 1813

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/gyl-9zb-5n9
title: Callophrys rubi (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/g1p-mpn-2nf
title: Oeneis jutta Hübner, 1806

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/fnb-3gb-tww
title: Amphipoea fucosa Freyer, 1830

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/yvd-ptj-zm0
title: Oeneis jutta Hübner, 1806

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/9x9-zkk-kyt
title: Amphipoea fucosa Freyer, 1830

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/rf0-mx2-jd4
title: Anarta (Calocestra) melanopa Thunberg, 1791

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/6f3-8yf-lww
title: Fabriciana adippe ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/yra-z5j-w5k
title: Zophodia convolutella Hübner, 1796

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/92m-clm-7lq
title: Lycaena Fabricius, 1807

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/jtd-fnc-lh2
title: Fabriciana adippe ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/b39-xde-lfb
title: Sideridis (Heliophobus) reticulata Goeze, 1781

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/hnt-be8-004
title: Parascotia fuliginaria (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/cw5-5f2-f3e
title: Sideridis (Heliophobus) reticulata Goeze, 1781

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/tzs-y3k-5cg
title: Lycaena phlaeas (Linnaeus, 1761)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/hdr-emx-g9w
title: Xestia (Megasema) c-nigrum (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/7m3-6rv-01w
title: Acronicta (Subacronicta) megacephala ([Denis & Schiffermüller], 1775)

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.3535/ak2-fq5-ph0
title: Sideridis (Heliophobus) reticulata Goeze, 1781

description: Digital Specimen for the physical specimen hosted at Finnish Museum of Natural History.
DOI: 10.5281/zenodo.17394690
title: Reproducible Research Platform for Genetic Engineering Player Bundle

description: RRP Genetic Engineering

Reproducible Research Platform (RRP) project for genetic engineering.

Overview

This RRP project repository demonstrates alternative, reproducible approaches to genetic engineering including molecular cloning and DNA editing. It provides reproducible workflows in contrast to desktop or cloud-based graphical user interfaces such as ApE, SnapGene, Genious or Benchling.

The main outcome of this example is:



In silico simulation of a CRISPR protocol.

A CRISPR plasmid charged with a target sequence for the yeast gene Myo1.

A PCR product with homologous recombination primers that amplify the fluorescent reporter (3x)mKate2.


PCR Result

This repository utilizes QUEEN [1] and Pydna [2] for DNA design and assembly. Detailed explanations of these tools can be found in their respective publications.

Features



Integration with RRP and openBIS ELN-LIMS: Mounts data (plasmids, oligos) from the research data management system openBIS in RRP for direct use.

Reproducible workflow: Automate genetic engineering tasks using Python-based tools.

Visualization: Generate detailed diagrams and results for plasmid construction and PCR products.


Installation

Prerequisites



Local Docker installation.


Steps



Download and extract the .zip file

Run the play script for your operating system



Linux / macOS: play

Windows: play.bat or play.ps1




Usage

In RRP open and run the Jupyter notebook MYO1_mKate2_CRISPR.ipynb

Workflow Overview



Plasmid Design: Use QUEEN to design CRISPR plasmids targeting Myo1.

PCR Primer Design: Generate primers for homologous recombination.

Visualization: View PCR fragment results using built-in plotting tools.


Directory Structure



├── .binder/ # Environment specifications, Python version, packages (QUEEN) etc.
├── .rrp/ # Specification of openBIS server and required data from RDMS openBIS ELN-LIMS (e.g. Plasmids)
├── docs/ # Documentation files
│ └── resources 
│   └── images
│     └── linearized_mKate2_fragment_w_Myo1_HR.png # Resources for the README
├── LICENSE # License
├── MYO1_mKate2_CRISPR.ipynb # Notebook demonstrating reproducible genetic engineering workflow.
└── README.md # This file


Authors and acknowledgment



Andreas P. Cuny, ETH Zurich initial implementation


Additionally, we acknowledge the use of QUEEN and Pydna tools in our workflows, as detailed in their respective publications.

License

This project is licensed under the Apache 2.0 License. See LICENSE file for details.

Copyright © 2020-2025 ETH Zurich, Andreas P. Cuny, D-BSSE, CSB Group

References

1: Mori, H., Yachie, N. A framework to efficiently describe and share reproducible DNA materials and construction protocols. Nat Commun 13, 2894 (2022). https://doi.org/10.1038/s41467-022-30588-x

2: Pereira, F., Azevedo, F., Carvalho, Â., Ribeiro, G. F., Budde, M. W., & Johansson, B. (2015). Pydna: a simulation and documentation tool for DNA assembly strategies using python. BMC Bioinformatics, 16(142), 142. https://doi.org/10.1186/s12859-015-0544-x