cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647796.v1
title: CIL:13873

description: Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows an aligned spindle, CIL #13889 shows a misaligned spindle and CIL# 13874 shows a rebudded cell) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.
DOI: 10.6084/m9.figshare.647798.v1
title: CIL:13889

description: Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a misaligned spindle, CIL #13873 shows an aligned spindle and CIL# 13874 shows a rebudded cell) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, middle panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.
DOI: 10.6084/m9.figshare.647793
title: CIL:13747

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL#13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells relocalizes Hh to apical cells (CIL#13746), but expression of Boi[∆FN1] that lacks the Hh-binding domain fails to rescue Hh localization (this image; boi[e]; UAS-Boi[∆FN1]/+;babGal4/+). Image is Fig 3D in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647794
title: CIL:13744

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium. In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells in the boi[e] mutant rescues Hh localization to apical cells (CIL# 13746). Image is Fig 3A in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647791
title: CIL:13745

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (this image). Expression of Boi in apical cells in the boi[e] mutant rescues Hh localization to apical cells (CIL# 13746); however expression of a Boi mutant that lacks the Hh binding domain does not (CIL# 13747). Image is Fig 3B in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647792
title: CIL:13746

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells in the boi[e] mutant (boi[e]; UAS-Boi/+; babGal4/+) rescues Hh localization to apical cells (this image). However, expression of a Boi mutant lacking the Hh-binding domain does not (CIL# 13747). Nuclei are labeled (DRAQ5, blue). Image is Fig 3C in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647794.v1
title: CIL:13744

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium. In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells in the boi[e] mutant rescues Hh localization to apical cells (CIL# 13746). Image is Fig 3A in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647790
title: CIL:40661

description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. This image shows the interaction of putative PSD from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, (this image) 2, and 3 are CIL 40661, 40662, and 40663, respectively.
DOI: 10.6084/m9.figshare.647793.v1
title: CIL:13747

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL#13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells relocalizes Hh to apical cells (CIL#13746), but expression of Boi[∆FN1] that lacks the Hh-binding domain fails to rescue Hh localization (this image; boi[e]; UAS-Boi[∆FN1]/+;babGal4/+). Image is Fig 3D in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647788
title: CIL:40659

description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Thick fibers in neurites represent microtubules. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image (this image) is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.
DOI: 10.6084/m9.figshare.647789
title: CIL:40660

description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Red asterisks indicate putative PSD fragments. Yellow boxes are enlarged in panels with corresponding numbers. Box 1, interaction of putative PSD (arrow) from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Boxes 2 and 3, branched actin filaments (cyan) in the head (2) and at the neck–head junction (3). Insets in panels 2 and 3 show nonpseudocolored regions outlined by yellow boxes. Dashed arrows in panel 3 indicate potential filament breakage. Bars, 0.2 μm. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.
DOI: 10.6084/m9.figshare.647791.v1
title: CIL:13745

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (this image). Expression of Boi in apical cells in the boi[e] mutant rescues Hh localization to apical cells (CIL# 13746); however expression of a Boi mutant that lacks the Hh binding domain does not (CIL# 13747). Image is Fig 3B in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647792.v1
title: CIL:13746

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells in the boi[e] mutant (boi[e]; UAS-Boi/+; babGal4/+) rescues Hh localization to apical cells (this image). However, expression of a Boi mutant lacking the Hh-binding domain does not (CIL# 13747). Nuclei are labeled (DRAQ5, blue). Image is Fig 3C in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
DOI: 10.6084/m9.figshare.647787
title: CIL:25822

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells deficient in endogenous CP, but rescued with EGFP-CP (KDR cells), were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, KDR cells exhibited dynamic changes on the dorsal surface of the cell, forming numerous protrusions as seen in the DIC time-lapse movie (CIL# 25821). This is a movie of a zoomed area of the irradiated region from CIL# 25821 and control movies are CIL#s 25823, 25824. This movie is Movie2 in Supporting Information and still images are in Fig 2B, top panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647788.v1
title: CIL:40659

description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Thick fibers in neurites represent microtubules. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image (this image) is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.
DOI: 10.6084/m9.figshare.647789.v1
title: CIL:40660

description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Red asterisks indicate putative PSD fragments. Yellow boxes are enlarged in panels with corresponding numbers. Box 1, interaction of putative PSD (arrow) from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Boxes 2 and 3, branched actin filaments (cyan) in the head (2) and at the neck–head junction (3). Insets in panels 2 and 3 show nonpseudocolored regions outlined by yellow boxes. Dashed arrows in panel 3 indicate potential filament breakage. Bars, 0.2 μm. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.
DOI: 10.6084/m9.figshare.647790.v1
title: CIL:40661

description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. This image shows the interaction of putative PSD from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, (this image) 2, and 3 are CIL 40661, 40662, and 40663, respectively.
DOI: 10.6084/m9.figshare.647784
title: CIL:21994

description: The aboral view of Didinium showing seven contractile vacuole pores for it's single posterior contractile vacuole and metachronous waves of cilia in one of the two characteristic ciliary girdles of Didinium nasutum. To one side you will note five white lines that represent rows of clavate cilia (also seen in CIL:17891 and CIL:19538). The bumps you will see on the surface at higher magnification may be either solitary clavate cilia or cyrtocysts, an extrusive organelle of unknown (possibly defensive) function. A thin section of a cyrtocyst can be seen at CIL:4665. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 825X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for qualitative analysis. Further details are available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447.
DOI: 10.6084/m9.figshare.647785
title: CIL:24812

description: Intracellular localization of GFP-Vps74 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutant, GFP-Vps74 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM as shown with colocalization of resident ER protein Sec63-mCherry (CIL# 24813). Thus, correct Golgi localization of Vps74 and GOLPH3 (see CIL#24815 for GFP-GOLPH3 in sac1∆ vps74∆ mutant) requires synthesis of PtdIns4P in the Golgi by Pik1-Frq1 and maintenance of low PtdIns4P levels in non-Golgi membranes by the Sac1 lipid phosphatase. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1B Vps74/sac1∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1B include CIL#s 24815, 24812, 24813.
DOI: 10.6084/m9.figshare.647786
title: CIL:24813

description: Sec63-mCherry in BY4742 sac1∆ vps74∆ highlights nuclear ER and cortical ER and/or PM. This image is the control image labeling ER for overlay with GFP-Vps74 (CIL# 24812) demonstrating that in the sac1∆ vps74∆ mutant, GFP-Vps74 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1B Sec63 (ER)/sac1∆ panel (overlay CIL#24812/24813 in merge panel) in J Cell Biol. 187: 967-975. 2009. Images in Fig 1B include CIL#s 24815, 24812, 24813.
DOI: 10.6084/m9.figshare.647787.v1
title: CIL:25822

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells deficient in endogenous CP, but rescued with EGFP-CP (KDR cells), were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, KDR cells exhibited dynamic changes on the dorsal surface of the cell, forming numerous protrusions as seen in the DIC time-lapse movie (CIL# 25821). This is a movie of a zoomed area of the irradiated region from CIL# 25821 and control movies are CIL#s 25823, 25824. This movie is Movie2 in Supporting Information and still images are in Fig 2B, top panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647783
title: CIL:13887

description: Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (CIL# 13884) and anaphase cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-BFA1–TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose. Image shows localization of Bfa1-Tem1 (eGFP, green) in anaphase after cells were transferred to medium with 2% glucose. Tem1 normally localizes preferentially to the SPB that enters the daughter cell during anaphase (CIL# 13882, 13885). Localization of Bfa1 is similar to that of Tem1, but Bfa1 is more stable on the SPB in the daughter cell and more asymmetric than Tem1 in late anaphase. The Bfa1-Tem1 fusion protein localization resembles Bfa1. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1B, bottom panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.
DOI: 10.6084/m9.figshare.647784.v1
title: CIL:21994

description: The aboral view of Didinium showing seven contractile vacuole pores for it's single posterior contractile vacuole and metachronous waves of cilia in one of the two characteristic ciliary girdles of Didinium nasutum. To one side you will note five white lines that represent rows of clavate cilia (also seen in CIL:17891 and CIL:19538). The bumps you will see on the surface at higher magnification may be either solitary clavate cilia or cyrtocysts, an extrusive organelle of unknown (possibly defensive) function. A thin section of a cyrtocyst can be seen at CIL:4665. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 825X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for qualitative analysis. Further details are available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447.
DOI: 10.6084/m9.figshare.647785.v1
title: CIL:24812

description: Intracellular localization of GFP-Vps74 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutant, GFP-Vps74 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM as shown with colocalization of resident ER protein Sec63-mCherry (CIL# 24813). Thus, correct Golgi localization of Vps74 and GOLPH3 (see CIL#24815 for GFP-GOLPH3 in sac1∆ vps74∆ mutant) requires synthesis of PtdIns4P in the Golgi by Pik1-Frq1 and maintenance of low PtdIns4P levels in non-Golgi membranes by the Sac1 lipid phosphatase. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1B Vps74/sac1∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1B include CIL#s 24815, 24812, 24813.
DOI: 10.6084/m9.figshare.647786.v1
title: CIL:24813

description: Sec63-mCherry in BY4742 sac1∆ vps74∆ highlights nuclear ER and cortical ER and/or PM. This image is the control image labeling ER for overlay with GFP-Vps74 (CIL# 24812) demonstrating that in the sac1∆ vps74∆ mutant, GFP-Vps74 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1B Sec63 (ER)/sac1∆ panel (overlay CIL#24812/24813 in merge panel) in J Cell Biol. 187: 967-975. 2009. Images in Fig 1B include CIL#s 24815, 24812, 24813.
DOI: 10.6084/m9.figshare.647780
title: CIL:13883

description: Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (this image) and anaphase cells (CIL#13886). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-CNM67–TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose. Image shows localization of Cnm67-Tem1 (eGFP, green) in metaphase after cells were transferred to medium with 2% glucose. Tem1 normally localizes preferentially to the SPB that enters the daughter cell during anaphase (CIL# 13882, 13885). Cnm67 is an integral component of the outer plaque of the SPB and the Cnm67-Tem1 fusion is found to be symmetric from SPB duplication until the end of mitosis. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1A, middle panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.
DOI: 10.6084/m9.figshare.647781
title: CIL:13884

description: Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (this image) and anaphase cells (CIL#13887). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-BFA1–TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose. Image shows localization of Bfa1-Tem1 (eGFP, green) in metaphase after cells were transferred to medium with 2% glucose. Tem1 normally localizes preferentially to the SPB that enters the daughter cell during anaphase (CIL# 13882, 13885). Localization of Bfa1 is similar to that of Tem1, but Bfa1 is more stable on the SPB in the daughter cell and more asymmetric than Tem1 in late anaphase. The Bfa1-Tem1 fusion protein localization resembles Bfa1. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1A, bottom panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.