cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/33y87-59e53
title: VSVGpNL4GFP entry HUVECs

description: HUVECs 30 min post spinoculation with VSVGpNL4GFP
description: <strong>Tilt Series Date:</strong> 2011-08-12</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Proviral construct from Irvin Chen UCLA</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG pNL4GFP by spinoculation</p>
<strong>Sample Preparation:</strong> manual blot</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-12-23/rawdata/</p>11aug12_batch_0019_ns.st,  Tilt Series (Pixel Size 0.5 nm), 999.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-12-23/rawdata/11aug12_batch_0019_ns.st"
        > <i class="download icon"></i> Download </a></p>

        11aug12_batch_0019_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 491.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-12-23/3dimage_54240/11aug12_batch_0019_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ke4gb-t4w33
title: E. coli (pLE6, 20 um IPTG)

description: e. coli from Larry Rothfield containing plasmid carrying WT MreB.
description: <strong>Tilt Series Date:</strong> 2011-07-29</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Sample Preparation:</strong> Induced with 20um IPTG for 2 hours starting at OD600=0.2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-07-29-14/3dimage_44271/</p>20110729_ple6_20umIPTG0006_part122_2.rec,  Reconstruction (Pixel Size 1.892 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-07-29-14/3dimage_44271/20110729_ple6_20umIPTG0006_part122_2.rec"
        > <i class="download icon"></i> Download </a></p>

        20110729_ple6_20umIPTG0006.mrc,  Tilt Series (Pixel Size 0.946 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-07-29-14/rawdata/20110729_ple6_20umIPTG0006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cpbm1-mya74
title: Acetonema longum

description: Vegetative septa of A. longum
description: <strong>Tilt Series Date:</strong> 2011-03-28</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Eric Matson grew the cells</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-03-28-22/3dimage_25374/</p>11mar28a_gr1_026_full.rec,  Reconstruction (Pixel Size 1.9 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-03-28-22/3dimage_25374/11mar28a_gr1_026_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11mar28a_gr1_026.mrc,  Tilt Series (Pixel Size 0.95 nm), 947.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-03-28-22/rawdata/11mar28a_gr1_026.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ezk7c-h0f81
title: E.coli "strain11" A413T CheAoff

description: E.coli methylation site mutant locked in a CHeA inactivating state
description: <strong>Tilt Series Date:</strong> 2011-03-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: °, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> mutant from John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown at 30C in TB to log phase, then treated with penicillin for one hour </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2011-03-04-4/rawdata/</p>11mar04a_coli11_012.st,  Tilt Series (Pixel Size 0.0 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2011-03-04-4/rawdata/11mar04a_coli11_012.st"
        > <i class="download icon"></i> Download </a></p>

        11mar04a_coli11_012.rec,  Reconstruction (Pixel Size 0.0 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2011-03-04-4/3dimage_15500/11mar04a_coli11_012.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0z3pv-0py78
title: correlated LMEM CTPsynthase

description: Raw data files of correlated LMEM CTPsynthase
description: <strong>Tilt Series Date:</strong> 2009-06-02</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: °, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zemer Gitai</p>
  aba2009-06-02-6_slicer9670.jpg: periplasmic vesicles, Caulobacter crescentus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2009-06-02-6/rawdata/</p>corrLMEM_CTP_cell6.st,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2009-06-02-6/rawdata/corrLMEM_CTP_cell6.st"
        > <i class="download icon"></i> Download </a></p>

        corrLMEM_CTP_cell6.rec,  Reconstruction (Pixel Size 1.9 nm), 243.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2009-06-02-6/3dimage_9127/corrLMEM_CTP_cell6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qrdh4-x5g33
title: sporulating MJ007 349

description: MJ007 349
description: <strong>Tilt Series Date:</strong> 2009-09-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> JFG349, ponA null, SpoIIIE73-11-GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation medium</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2009-09-27-28/rawdata/</p>09sep27a_et-pos3_003.mrc,  Tilt Series (Pixel Size 0.95 nm), 998.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2009-09-27-28/rawdata/09sep27a_et-pos3_003.mrc"
        > <i class="download icon"></i> Download </a></p>

        cell3_full.rec,  Reconstruction (Pixel Size 1.9 nm), 280.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2009-09-27-28/3dimage_25870/cell3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/epj74-dbx84
title: 1ug chloramphenicol

description: Raw data files of 1ug chloramphenicol
description: <strong>Tilt Series Date:</strong> 2013-12-11</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
  psa2013-12-11-22_slicer4150.jpg: Nested Vesicle psa2013-12-11-22_slicer4151.jpg: Granules
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2013-12-11-22/rawdata/</p>atlas20011.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2013-12-11-22/rawdata/atlas20011.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas20011_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2013-12-11-22/3dimage_35979/atlas20011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/421rc-xx532
title: E.coli   "strain 2619" EEEE

description: E.coli   "strain 2619" EEEE tsr receptor methylation site mutant EEEE CheA off state (CCWA)
description: <strong>Tilt Series Date:</strong> 2012-04-02</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB medium at 30 degrees, 1 hour lysed with Penicillin</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-04-02-27/rawdata/</p>UU2619_marchb0001.mrc,  Tilt Series (Pixel Size 0.644 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-04-02-27/rawdata/UU2619_marchb0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        UU2619_marchb0001_full.rec,  Reconstruction (Pixel Size 1.288 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-04-02-27/3dimage_18103/UU2619_marchb0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vhasj-j6470
title: L256T chemotaxis mutant

description: L256T HAMP mutant locked in an 'CheA activating state'
description: <strong>Tilt Series Date:</strong> 2010-03-18</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> John Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in TB media with Chloramphenicol, induced with 0.6µM salicylate for 3 hours, penicillin treated for 1 hour</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-03-18-34/rawdata/</p>10mar29a_EcoliL256T_024.st,  Tilt Series (Pixel Size 0.632 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-03-18-34/rawdata/10mar29a_EcoliL256T_024.st"
        > <i class="download icon"></i> Download </a></p>

        10mar29a_EcoliL256T_024_full.rec,  Reconstruction (Pixel Size 0.632 nm), 623.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-03-18-34/3dimage_12757/10mar29a_EcoliL256T_024_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2yeb1-j6044
title: Vibrio cholerae

description: 2011_05_24_
description: <strong>Tilt Series Date:</strong> 2011-05-24</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> 2740-80</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 0.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> </p>
<strong>Collaborators and Roles:</strong> Mekalanos</p>
  mpa2011-05-24-8_slicer7266.jpg: ring mpa2011-05-24-8_slicer7268.jpg: tube mpa2011-05-24-8_slicer7269.jpg: filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2011-05-24-8/rawdata/</p>2011_05_24_10.mrc,  Tilt Series (Pixel Size 0.0 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2011-05-24-8/rawdata/2011_05_24_10.mrc"
        > <i class="download icon"></i> Download </a></p>

        2011_05_24_10_full.rec,  Reconstruction (Pixel Size 0.0 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2011-05-24-8/3dimage_34854/2011_05_24_10_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kcg1w-bkc07
title: MLV 43D

description: MLV, wild type, WT, 2nd batch
description: <strong>Tilt Series Date:</strong> 2014-04-08</p>
<strong>Data Taken By:</strong> Luiza Mendonca</p>
<strong>Species / Specimen:</strong> MLV</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Hung Fan</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lmm2014-04-08-29/rawdata/</p>L018_MLV_43D_10009.mrc,  Tilt Series (Pixel Size 0.6 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lmm2014-04-08-29/rawdata/L018_MLV_43D_10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        L018_MLV_43D_10009_part81_1.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lmm2014-04-08-29/3dimage_41219/L018_MLV_43D_10009_part81_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/phb05-k6e49
title: M. xanthus (Dk1622)

description: Imaged for Lotte in search of T6SS. This is the WT strain that they work with.
description: <strong>Tilt Series Date:</strong> 2012-08-17</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 23000x. </p>
<strong>Microscope:</strong> UCLA Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Lotte Soregard-Anderson</p>
  mst2012-08-17-32_slicer4448.jpg: Granules
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2012-08-17-32/rawdata/</p>081712_2_DK1622_13.mrc,  Tilt Series (Pixel Size 1.11 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2012-08-17-32/rawdata/081712_2_DK1622_13.mrc"
        > <i class="download icon"></i> Download </a></p>

        081712_2_DK1622_13_full.rec,  Reconstruction (Pixel Size 2.22 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2012-08-17-32/3dimage_43455/081712_2_DK1622_13_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m5aw4-2wn64
title: Sacculi Protochlamydia amoebophila

description: Sacculus preparation
description: <strong>Tilt Series Date:</strong> 2013-01-04</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Protochlamydia amoebophila</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Matthias Horn</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified sacculi</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-01-04-17/rawdata/</p>01042013_MP116_0009.st,  Tilt Series (Pixel Size 1.23 nm), 215.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-01-04-17/rawdata/01042013_MP116_0009.st"
        > <i class="download icon"></i> Download </a></p>

        01042013_MP116_0009.rec,  Reconstruction (Pixel Size 2.47 nm), 22.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-01-04-17/3dimage_31807/01042013_MP116_0009.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6t235-y0y81
title: B subtilis bMD208

description: (Ω) cat::Pxyl-gfp-mreB  ΔponA::kan 30 mM xyloseNotes from Mike Dion:clump mildly in liquid LB.  This can be easily disrupted by very mild vortexing, or growing them in the presence of 10 mM MgCl2.  The clumping is the result of their ponA deletionbMD208 and 210 are Graumann's strains that I just recently knocked ponA out of.  These strains have the mut1 variant of GFP that, in addition to mut1, is also L236P.  I determined this by sequencing both of Gruamann's strains.  Both of these grow very slow on LB agar (bMD208 grows worse than bMD210, on LB agar)
description: <strong>Tilt Series Date:</strong> 2013-12-04</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> bMD208</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Ethan Garner and Mike Dion</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-12-04-23/rawdata/</p>120413_bs208_40014.mrc,  Tilt Series (Pixel Size 0.42 nm), 1.7 GB  
        
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        120413_bs208_40014_full.rec,  Reconstruction (Pixel Size 1.68 nm), 861.2 MB  
        
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