cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7513133.v1
title: Physiological and isozyme alterations in lettuce seeds under different conditions and storage periods

description: Abstract: The objective of the study was to determine the physiological and isozyme alterations in lettuce seeds submitted to different conditions and storage periods. We used a completely randomized design with four replications in a factorial arrangement with six lettuce cultivars (Everglades, Babá de Verão, Elisa, Grand Rapids, Hortência and Salinas 88) and four storage periods (30, 60, 90 and 120 days), on three storage rooms (15, 25 and 35 ° C). The physiological quality was evaluated by first and final germination count, emergence, and germination and emergence speed indexes. The isoenzyme expression of catalase (CAT), esterase (EST), alcohol dehydrogenase (ADH) and malate dehydrogenase (MDH) were determined. The physiological quality of lettuce seeds was preserved when stored at 15 ° C room temperature. The high room temperatures associated with higher storage periods cause a reduction in physiological quality and alterations in enzymatic patterns of CAT, EST, ADH and MDH. For seeds stored at 15 ° C, there were no physiological changes. The lettuce cultivars, especially Everglades, have different levels of tolerance to storage and isoenzyme expressions.
DOI: 10.6084/m9.figshare.7516982
title: Correlation of biometrical characteristics of fruit and seed with twinning and vigor of Prunus persica rootstocks

description: Abstract: This study aimed at determining and correlating the main morphometric characteristics of fruits and seeds with the germination potential and vigor of eight peach rootstocks. The experimental design was completely randomized, with four replications of 50 seeds per treatment. The analyzed variables were: length, width, thickness, fresh mass of fruit and seeds, moisture content of seeds, percentage of seeds attacked by fungi and intact seeds, germination percentage, germination speed index (GSI) and mean germination time (MGT). The Capdeboscq, Aldrighi and Tsukuba 1 cultivars showed higher values of length, width, thickness and fresh mass of seeds compared to other cultivars, presenting a relationship with the later period of fruit ripening. Regardless of the fruit ripening time, the germination percentage was high, ranging between 93% and 100% for all cultivars. In addition, the fresh mass of fruits has showed a high positive correlation with the fresh mass of seeds for the eight evaluated peach rootstocks. The GSI and MGT have a high relationship with the fresh mass of seeds.
DOI: 10.6084/m9.figshare.7508837
title: Alternative treatment to stimulate the germination of recently harvested wheat seeds

description: Abstract: The germination test for recently harvested wheat seeds requires a period of up to 20 days, because of the presence of dormancy. The possibility of reducing this period provides greater speed to the allocation of seeds, as well as an increased operational capacity of analytical laboratories. This study aimed at evaluating the effectiveness of treatments to stimulate the germination of recently harvested wheat seeds, reducing the five-day pre-cheeling period recommended to run the test. Seeds from various wheat genotypes were used: BRS Guamirim, BRS Tangará, BRS Pardela, CD 108, CD 116, CD 150, IPR Catuara TM, Frontana, IPR Taquari, Safira and Quartzo. They were analyzed immediately after collection and then stored for 30 and 180 days. In each evaluation period (immediately after harvesting, 30 and 180 days of storage), the determination of water content, the germination test (seeds without pre-chilling and pre-chilling between 5 and 10 ° C for three to five days), and also the tetrazolium test to quantify viability and seed dormancy were carried out. Based on these results, it is concluded that there is effectiveness in overcoming wheat seed dormancy, adopting the pre-cheeling procedure between 5 and 10 ° C for three days.
DOI: 10.6084/m9.figshare.7513298
title: Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

description: Abstract: Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.
DOI: 10.6084/m9.figshare.7518878
title: Common bean seed vigor affecting crop grain yield

description: Abstract: Among the factors that affect seedling emergence and crop establishment are seed germination and vigor, which has an important influence on the establishment of the initial plant population and development, and may affect crop yield. Based on this background the objective of this research work was to evaluate the effects of seed vigor of common bean seed lots and its effects on crop grain yield. The treatments consisted of six seed lots of common bean, which were composed by different combinations of high and low vigor seeds. Seed lots were assessed by germination and vigor tests (first count of germination, electrical conductivity and accelerated aging). A field experiment was conducted in a randomized block design, with plots of six lines of five meters and were evaluated for grain yield at harvest. It was concluded that seed vigor affects common bean grain yield; the use of low vigor seeds results in grain yield reductions of up to 20%.
DOI: 10.6084/m9.figshare.7511663
title: Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings

description: Abstract: Phytelephas macrocarpa (ivory palm) is an Amazonian palm vulnerable to exploitation pressure, as its seeds are widely used in regional handicrafts. The aims of this study were to evaluate the effectiveness of different stratification temperatures in overcoming seed dormancy and to analyze the seedling development stages of this species. In germination under stratification, the seeds were placed in plastic bags containing moistened vermiculite, and maintained at the constant temperatures of 25, 30, 35 and 40 °C, and an alternating temperature from 26 to 40 °C. In the study about the development of seedlings, seeds were sown in vermiculite under plastic cover (growing house), and the evolution of the seedling was evaluated, from the formation of germinative button to the complete blade expansion of the first eophyll. Stratification at alternating temperatures (26 to 40 °C) helped overcoming seed dormancy. Stratification at 25 °C kept the seed viability for nine months. Germination, characterized by the formation of the germinative button took an average of 114 ± 24 days, and the seedling development until the first extended eophyll lasted 244 ± 57 days.
DOI: 10.6084/m9.figshare.7507481
title: Aspects of Peltophorum dubium Sprengel (Taubert) seeds in an aerial seed bank

description: Abstract: Knowledge concerning natural regeneration strategies is important due to the effect of natural regeneration on the local ecosystem. The aim of this study was to examine the ecophysiological aspects of the Peltophorum dubium aerial seed bank. Fruits were harvested monthly, before and after seed maturation, from selected trees in the municipality of Lavras, MG, Brazil, for determination of moisture content, acquisition of tolerance to desiccation, seed viability, maintenance of dormancy, and mean germination time. Physiological maturity of the species was attained in June, when low moisture content, maximum dry matter, and high germination percentage were observed. We found that there was no change in dormancy, in germination percentage, or in the protein profile of seeds during the period they remained in the aerial seed bank. The P. dubium species creates an aerial seed bank of short serotiny, with fruits remaining attached to the tree for 9 months.
DOI: 10.6084/m9.figshare.7508837.v1
title: Alternative treatment to stimulate the germination of recently harvested wheat seeds

description: Abstract: The germination test for recently harvested wheat seeds requires a period of up to 20 days, because of the presence of dormancy. The possibility of reducing this period provides greater speed to the allocation of seeds, as well as an increased operational capacity of analytical laboratories. This study aimed at evaluating the effectiveness of treatments to stimulate the germination of recently harvested wheat seeds, reducing the five-day pre-cheeling period recommended to run the test. Seeds from various wheat genotypes were used: BRS Guamirim, BRS Tangará, BRS Pardela, CD 108, CD 116, CD 150, IPR Catuara TM, Frontana, IPR Taquari, Safira and Quartzo. They were analyzed immediately after collection and then stored for 30 and 180 days. In each evaluation period (immediately after harvesting, 30 and 180 days of storage), the determination of water content, the germination test (seeds without pre-chilling and pre-chilling between 5 and 10 ° C for three to five days), and also the tetrazolium test to quantify viability and seed dormancy were carried out. Based on these results, it is concluded that there is effectiveness in overcoming wheat seed dormancy, adopting the pre-cheeling procedure between 5 and 10 ° C for three days.
DOI: 10.6084/m9.figshare.7511663.v1
title: Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings

description: Abstract: Phytelephas macrocarpa (ivory palm) is an Amazonian palm vulnerable to exploitation pressure, as its seeds are widely used in regional handicrafts. The aims of this study were to evaluate the effectiveness of different stratification temperatures in overcoming seed dormancy and to analyze the seedling development stages of this species. In germination under stratification, the seeds were placed in plastic bags containing moistened vermiculite, and maintained at the constant temperatures of 25, 30, 35 and 40 °C, and an alternating temperature from 26 to 40 °C. In the study about the development of seedlings, seeds were sown in vermiculite under plastic cover (growing house), and the evolution of the seedling was evaluated, from the formation of germinative button to the complete blade expansion of the first eophyll. Stratification at alternating temperatures (26 to 40 °C) helped overcoming seed dormancy. Stratification at 25 °C kept the seed viability for nine months. Germination, characterized by the formation of the germinative button took an average of 114 ± 24 days, and the seedling development until the first extended eophyll lasted 244 ± 57 days.
DOI: 10.6084/m9.figshare.7507982
title: Effect of temperature on passion fruit emergence and seedling vigor

description: Abstract: Seedlings for commercial growing of passion fruit are preferentially acquired through seeds. Success in germination and seedling emergence, in turn, is influenced by the genetic constitution of the progenies and the thermal conditions imposed on the growth environment. The aim of this study was to evaluate the emergence and vigor of seedlings of passion fruit cultivars exposed to different temperatures. The experiment was conducted in a germination chamber at the Unidade de Ensino, Pesquisa e Extensão / Pomar Campus of the Department of Plant Science of the Universidade Federal de Viçosa (UFV). The study was carried out in 5 × 3 factorial arrangement, with five temperature ranges (5-15, 10-20, 15-25, 20-30 and 25-35 °C) and three varieties of passion fruit (BRS Sol do Cerrado, BRS Gigante Amarelo and FB-200 Yellow Master) in a completely randomized design with six replications of 25 seeds per plot. The variables analyzed were the percentage, the speed index, and the average time of emergence, shoot length, total length, and seedling dry matter. The cultivars BRS Sol do Cerrado and BRS Gigante Amarelo exhibited seedling emergence of over 95% in the temperature ranges of 20-30 °C and 25-35 °C. Alternating temperatures from 20-30 °C stimulates seedling emergence, seedling growth, and greater accumulation of seedling dry matter.
DOI: 10.6084/m9.figshare.7507481.v1
title: Aspects of Peltophorum dubium Sprengel (Taubert) seeds in an aerial seed bank

description: Abstract: Knowledge concerning natural regeneration strategies is important due to the effect of natural regeneration on the local ecosystem. The aim of this study was to examine the ecophysiological aspects of the Peltophorum dubium aerial seed bank. Fruits were harvested monthly, before and after seed maturation, from selected trees in the municipality of Lavras, MG, Brazil, for determination of moisture content, acquisition of tolerance to desiccation, seed viability, maintenance of dormancy, and mean germination time. Physiological maturity of the species was attained in June, when low moisture content, maximum dry matter, and high germination percentage were observed. We found that there was no change in dormancy, in germination percentage, or in the protein profile of seeds during the period they remained in the aerial seed bank. The P. dubium species creates an aerial seed bank of short serotiny, with fruits remaining attached to the tree for 9 months.
DOI: 10.6084/m9.figshare.7513298.v1
title: Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

description: Abstract: Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.
DOI: 10.6084/m9.figshare.5671018.v1
title: Embryonic dormancy in seeds of Bactris gasipaes Kunth (peach-palm)

description: Abstract: Bactris gasipaes is a domesticated palm whose fruits are of great importance for the Amazonian people and whose heart of palm is also receiving economic interest in other brazilian and Latin America regions. The aim of this study was verify embryonic dormancy and its correlation with first cataphyll emergence in B. gasipaes seeds collected from four plants at Manaus city and four others at Coari city, both in the Amazonas state, Brazil. After extraction and cleaning, some of the seeds (4 replications of 25 per plant) were sown in a seedbed with a sawdust and sand mixture as substrate, and embryos (4 replications of 10 per plant), after extraction, were inoculated into half strength Murashige and Skoog cultures. Were used 100 seeds and 40 embryo per treatment. Whole seed and embryo germination varied between the different source plants and locations, with the greatest difference observed for the emergence of first cataphyll from seeds in the seedbed. For the most part of variables, results of seed and embryo were positively associated, namely, as one went up the other also, and vice versa. These results suggesting that, at least in part, seed dormancy in Bactris gasipaes is associated with embryonic dormancy.
DOI: 10.6084/m9.figshare.7507982.v1
title: Effect of temperature on passion fruit emergence and seedling vigor

description: Abstract: Seedlings for commercial growing of passion fruit are preferentially acquired through seeds. Success in germination and seedling emergence, in turn, is influenced by the genetic constitution of the progenies and the thermal conditions imposed on the growth environment. The aim of this study was to evaluate the emergence and vigor of seedlings of passion fruit cultivars exposed to different temperatures. The experiment was conducted in a germination chamber at the Unidade de Ensino, Pesquisa e Extensão / Pomar Campus of the Department of Plant Science of the Universidade Federal de Viçosa (UFV). The study was carried out in 5 × 3 factorial arrangement, with five temperature ranges (5-15, 10-20, 15-25, 20-30 and 25-35 °C) and three varieties of passion fruit (BRS Sol do Cerrado, BRS Gigante Amarelo and FB-200 Yellow Master) in a completely randomized design with six replications of 25 seeds per plot. The variables analyzed were the percentage, the speed index, and the average time of emergence, shoot length, total length, and seedling dry matter. The cultivars BRS Sol do Cerrado and BRS Gigante Amarelo exhibited seedling emergence of over 95% in the temperature ranges of 20-30 °C and 25-35 °C. Alternating temperatures from 20-30 °C stimulates seedling emergence, seedling growth, and greater accumulation of seedling dry matter.
DOI: 10.6084/m9.figshare.7513133
title: Physiological and isozyme alterations in lettuce seeds under different conditions and storage periods

description: Abstract: The objective of the study was to determine the physiological and isozyme alterations in lettuce seeds submitted to different conditions and storage periods. We used a completely randomized design with four replications in a factorial arrangement with six lettuce cultivars (Everglades, Babá de Verão, Elisa, Grand Rapids, Hortência and Salinas 88) and four storage periods (30, 60, 90 and 120 days), on three storage rooms (15, 25 and 35 ° C). The physiological quality was evaluated by first and final germination count, emergence, and germination and emergence speed indexes. The isoenzyme expression of catalase (CAT), esterase (EST), alcohol dehydrogenase (ADH) and malate dehydrogenase (MDH) were determined. The physiological quality of lettuce seeds was preserved when stored at 15 ° C room temperature. The high room temperatures associated with higher storage periods cause a reduction in physiological quality and alterations in enzymatic patterns of CAT, EST, ADH and MDH. For seeds stored at 15 ° C, there were no physiological changes. The lettuce cultivars, especially Everglades, have different levels of tolerance to storage and isoenzyme expressions.
DOI: 10.6084/m9.figshare.5671258
title: Assessment of squash seed vigor using computerized image analysis

description: Abstract: The objective of this study was to determine the efficacy of the SVIS® (Seed Vigor Imaging System) to determine squash seed vigor evaluated at different stages of maturation. ‘Jabras’ hybrid seeds, resulting from a cross between Cucurbita maxima (female parent) and Cucurbita moschata (male parent), were used in this study. Seeds extracted from fruit at different maturation stages (harvested at 15, 30, 45, 60 and 75 days after anthesis and stored or not for 20 days after each harvest) were subjected to the following determinations: 100 seed weight, germination, saturated salt accelerated aging, and SVIS®. This computerized system provided data on indexes of seedling vigor and uniformity of seedling development and on seedling growth. Results from SVIS® were similar to information from the other vigor tests performed in this study, detecting significant differences in performance among seeds during the maturation process. This indicates that SVIS® is a reliable alternative for obtaining consistent information on the physiological potential of squash seeds.
DOI: 10.6084/m9.figshare.5644540
title: Evaluation of inoculum potential of pathogens in seeds: relation to physiological quality and DNA quantification by qPCR

description: Abstract: Given what is already known in regard to seed health and the availability of molecular methods for detection of the pathogens Stenocarpella maydis and Stenocarpella macrospora in maize seeds, Colletotrichum gossypii var. cephalosporioides in cotton seeds, and Corynespora cassiicola in soybean seeds, the aim of this study was to evaluate seed vigor according to different inoculum potentials. The fungus isolates were inoculated on seeds by the technique of water restriction, through which different inoculum potentials are obtained, corresponding to times of seed exposure of 0, 24, 48, and 96 hours for maize and cotton seeds, and 0, 36, 108, and 144 hours for soybean seeds. The seeds were subjected to germination, electrical conductivity, health, and qPCR tests. Results of the blotter test showed that in most pathosystems, there was a higher incidence of the fungi with an increase in inoculum potential. A decrease in germination percentage was observed in all species as inoculum potential increased, as well as further degradation of seed membranes. The qPCR test confirmed that the most damaged seeds in the tests had higher presence of the pathogens.
DOI: 10.6084/m9.figshare.5670667
title: Changes in desiccation tolerance and respiratory rates of immature Caesalpinia echinata Lam. Seeds

description: Abstract: Seed storage is an important tool for ex situ conservation. Orthodox seeds can be stored for long periods, but recalcitrant seeds generally only for short periods. There is wide variation in the degree of desiccation tolerance between orthodox and recalcitrant seeds, leading authors to suggest levels of recalcitrance and, more recently, that there are variations in the stage of maturity at seed dispersal. Thus, recalcitrant behavior would be a result of premature dispersal from the mother plant. In this study, we sought to establish physiological relationships between different stages of development of immature orthodox Caesalpinia echinata seeds and the stages described for recalcitrant seeds to verify similarity of behavior. Therefore, we analyzed the desiccation tolerance of seeds collected at different ages, with and without PEG treatment, at different levels of drying (40%, 30%, 20% and 10% water content, wet basis). Changes in water potential, germination, vigor, and respiratory rates of the seeds were analyzed, and the results showed that: 1) desiccation tolerance increased as maturation proceeded; 2) PEG treatment did not induce desiccation tolerance; and 3) PEG treatment decreased the seed oxidation rate, which is the main factor in rapid seed deterioration of C. echinata in storage.