DOI: 10.7910/dvn/czp3jp
title: Experimental Assessment of Active Homeostasis Regulation for Extending Mammalian Lifespan by Prolonging the Youth Phase

description: Idea of the Work /1/:
Hypothesis 1: Within a living organism, the fluids forming the basis of intercellular and intracellular fluids, plasma and lymph, are chemically H₂O, yet their physical properties differ fundamentally from those of drinking water. For example, physiological saline, which closely mimics the chemical composition of blood plasma, can only be intravenously administered in strictly limited amounts and at a controlled rate, due to its significant physical disparity from blood plasma.
Moreover, within the body, the physical parameters of fluids are maintained in a non-equilibrium state, requiring constant effort from the organism to keep them within the so-called physiological norm, a phenomenon referred to as homeostasis in biology and medicine.
Hypothesis 2: The physical characteristics of homeostasis serve as the foundation for its biochemical parameters. Therefore, maintaining these physical parameters actively within the physiological corridor substantially aids the organism in stabilizing its biochemical homeostasis and reduces the overall energy expenditure required to do so.
Hypothesis 3: Active regulation of the physical parameters of homeostasis, ensuring they remain within physiological limits throughout all stages of life, including under adverse internal and external influences, allows the organism to conserve energy and biological resources during its lifespan.
If these hypotheses hold true, then active homeostasis control enables the body’s cells to exist in a "gentle mode." This would allow the cells to complete their life cycles in the time naturally allotted to them. As a result, cell divisions in the experimental group occur less frequently compared to the control group. Given the biological limit on cell division (e.g., telomere shortening with each division), cells—and therefore organs—in the experimental group would not only live longer but also retain youthful characteristics for a significantly extended period.
description: A characteristic example A representative case of homeostasis restoration in a patient with an acute inflammatory process in both kidneys, bilateral kidney inflammation complicated by high fever, is provided as an illustration example. 
The following criteria were adopted for preserving to assess the preservation of the youth phase in mice:
- the state of the brain, characterized by the level of the aging factor in blood serum;

- the state of the reproductive function and the structure of some internal organs (based on histological analysis) in experimental mice at advanced stages of life, by which time the mice in the control groups had already passed away;

- fertility in experimental mice at later stages of life.

The results of the studies provide comparative data on the level of the aging factor in the blood serum of control and experimental animals, as well as average and maximum life expectancy, the birth of offspring at late stages of life, and the histological analysis of internal organs in 28-month-old mice from the experimental group, which significantly outlived the control group animals.
The histological analysis of the lungs, heart, cerebral cortex, ovaries, lymph nodes, and thymus confirmed the preservation of structural and functional characteristics of these organs in the experimental group animals. The ovarian histology explained the continued reproductive activity of the experimental group and the occurrence of offspring even at advanced stages of life.