cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11351045
title: BRS FC104 - Super-early carioca seeded common bean cultivar with high yield potential

description: Abstract BRS FC104 is a carioca seeded common bean cultivar with super-early maturity (mean of 65 days; emergence to physiological maturity) and disease resistance superior to other early maturity carioca seeded cultivars. Is is recommended for growing in 21 Brazilian states and has high yield potential (3792 kg ha-1), a mean yield of 2186 kg ha-1, and 6.9% higher mean of the relative yield compared to the control cultivars.
DOI: 10.6084/m9.figshare.11351045.v1
title: BRS FC104 - Super-early carioca seeded common bean cultivar with high yield potential

description: Abstract BRS FC104 is a carioca seeded common bean cultivar with super-early maturity (mean of 65 days; emergence to physiological maturity) and disease resistance superior to other early maturity carioca seeded cultivars. Is is recommended for growing in 21 Brazilian states and has high yield potential (3792 kg ha-1), a mean yield of 2186 kg ha-1, and 6.9% higher mean of the relative yield compared to the control cultivars.
DOI: 10.6084/m9.figshare.12056373
title: Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR

description: Abstract A large collection of coffee genetic resources is conserved in Costa Rica. In this study, microsatellite DNA fingerprinting of coffee through singleplex and multiplex PCR approaches coupled with capillary electrophoresis are described. To validate both methods, germplasm of Coffea spp. (Arabica and non-Arabica) and intraspecific F1 hybrids were analyzed using fourteen microsatellite markers. It was observed that through both PCR methods the fingerprinting profile of a subset of samples was identical. The genetic analyses revealed that non-Arabica coffee displayed greater genetic variation than Arabica coffee did. In addition, microsatellite analyses allowed the separation of C. arabica from other species using the principal coordinate analysis (PCoA) approach. The neighbor-joining tree clustering analysis revealed either a grouping of wild genotypes separated from cultivars of C. arabica, or a relation of intraspecific F1 hybrids with parental lines. The utility of our methodology for the characterization of F1 hybrids not previously analyzed through SSR (Simple Sequence Repeats) fingerprinting is demonstrated.
DOI: 10.6084/m9.figshare.12056373.v1
title: Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR

description: Abstract A large collection of coffee genetic resources is conserved in Costa Rica. In this study, microsatellite DNA fingerprinting of coffee through singleplex and multiplex PCR approaches coupled with capillary electrophoresis are described. To validate both methods, germplasm of Coffea spp. (Arabica and non-Arabica) and intraspecific F1 hybrids were analyzed using fourteen microsatellite markers. It was observed that through both PCR methods the fingerprinting profile of a subset of samples was identical. The genetic analyses revealed that non-Arabica coffee displayed greater genetic variation than Arabica coffee did. In addition, microsatellite analyses allowed the separation of C. arabica from other species using the principal coordinate analysis (PCoA) approach. The neighbor-joining tree clustering analysis revealed either a grouping of wild genotypes separated from cultivars of C. arabica, or a relation of intraspecific F1 hybrids with parental lines. The utility of our methodology for the characterization of F1 hybrids not previously analyzed through SSR (Simple Sequence Repeats) fingerprinting is demonstrated.
DOI: 10.6084/m9.figshare.12056355
title: Guava breeding via full-sib family selection: conducting selection cycle and divergence between parents and families

description: Abstract The major hindrance to guava fruit (Psidium guajava L.) growing is the low availability of cultivars for use by Brazilian producers, who usually rely on few options of genotypes to implement the crop. In the present study, 11 full-sib families were evaluated in a randomized-block experimental design with three replicates, in order to more efficiently select superior genotypes. Genetic parameters were estimated and the best genotypes were selected based on genetic value by applying the REML/BLUP statistical procedure. Additionally, genetic divergence was estimated based on the mean Euclidian distance between the individuals selected via BLUP. Based on genetic divergence, the best genotypes were selected for use as parents in new crosses aiming at continuity of the guava breeding program. The mean values of the traits of selected individuals surpassed the mean values of their parents, confirming that the strategy of obtaining full-sib families is effective in generating considerable gains.
DOI: 10.6084/m9.figshare.12056376
title: Prediction of grain yield, adaptability, and stability in landrace varieties of lima bean (Phaseolus lunatus L.)

description: Abstract The aim of this study was to compare the Multiple Linear Regression and Artificial Neural Network models in prediction of grain yield of ten landrace varieties of lima bean and evaluate adaptability and stability through the Lin and Binns method for identification of the best performing variety. Trials were conducted in the municipalities of Teresina, PI, and São Domingos do Maranhão, MA, through measurement of 12 traits, except for grain yield in São Domingos do Maranhão. The parameters of Pearson and Spearman correlation, root mean square error, mean absolute error, and coefficient of determination were used to compare the models. The Artificial Neural Network proved to be more adequate for prediction of grain yield. Adaptability and stability analyses indicated that the environments are discriminant for selection of promising genotypes, and that the landrace variety Mulatinha can be recommended for planting in the municipalities.
DOI: 10.6084/m9.figshare.12056376.v1
title: Prediction of grain yield, adaptability, and stability in landrace varieties of lima bean (Phaseolus lunatus L.)

description: Abstract The aim of this study was to compare the Multiple Linear Regression and Artificial Neural Network models in prediction of grain yield of ten landrace varieties of lima bean and evaluate adaptability and stability through the Lin and Binns method for identification of the best performing variety. Trials were conducted in the municipalities of Teresina, PI, and São Domingos do Maranhão, MA, through measurement of 12 traits, except for grain yield in São Domingos do Maranhão. The parameters of Pearson and Spearman correlation, root mean square error, mean absolute error, and coefficient of determination were used to compare the models. The Artificial Neural Network proved to be more adequate for prediction of grain yield. Adaptability and stability analyses indicated that the environments are discriminant for selection of promising genotypes, and that the landrace variety Mulatinha can be recommended for planting in the municipalities.
DOI: 10.6084/m9.figshare.12056367
title: Reproductive analyses of intraspecific Paspalum notatum Flügge hybrids.

description: Abstract Paspalum notatum is an important forage native to Southern Brazil and one of the most promising fodder species in terms of productivity, quality, resistance and growth speed. The objective of this study was to evaluate the reproductive mode and fertility of hybrid progenies resulting from artificial crosses among artificially duplicated sexual plants, selected intraspecific hybrids of the UFRGS breeding program and apomictic ecotypes of the species. The resulting F1 progenies were represented by 24 plants with high pollen viability (varying from 82.15 to 99.67%) and 1.2:1 segregation for the sexual: apomictic reproduction mode, indicating predominantly sexual hybrids, due to distorted segregation. In the apomictic plants, most chromosomes paired as bivalents in diakinesis. Since the low presence of abnormalities observed in the meiotic chromosome pairing and the high pollen viability of the hybrids, all plants were confirmed as promising parents for future crosses.
DOI: 10.6084/m9.figshare.12056367.v1
title: Reproductive analyses of intraspecific Paspalum notatum Flügge hybrids.

description: Abstract Paspalum notatum is an important forage native to Southern Brazil and one of the most promising fodder species in terms of productivity, quality, resistance and growth speed. The objective of this study was to evaluate the reproductive mode and fertility of hybrid progenies resulting from artificial crosses among artificially duplicated sexual plants, selected intraspecific hybrids of the UFRGS breeding program and apomictic ecotypes of the species. The resulting F1 progenies were represented by 24 plants with high pollen viability (varying from 82.15 to 99.67%) and 1.2:1 segregation for the sexual: apomictic reproduction mode, indicating predominantly sexual hybrids, due to distorted segregation. In the apomictic plants, most chromosomes paired as bivalents in diakinesis. Since the low presence of abnormalities observed in the meiotic chromosome pairing and the high pollen viability of the hybrids, all plants were confirmed as promising parents for future crosses.
DOI: 10.6084/m9.figshare.12056937
title: BRS 372 and BRS 416: high-yielding cotton cultivars with multiple disease resistance

description: Abstract BRS 372 and BRS 416 are cotton cultivars with high lint yield and genetic resistance to the main cotton diseases in Brazil, particularly to Ramularia Leaf Spot. They have stable yields, good fiber quality and can contribute to a sustainable cotton disease management in tropical environments.
DOI: 10.6084/m9.figshare.12056937.v1
title: BRS 372 and BRS 416: high-yielding cotton cultivars with multiple disease resistance

description: Abstract BRS 372 and BRS 416 are cotton cultivars with high lint yield and genetic resistance to the main cotton diseases in Brazil, particularly to Ramularia Leaf Spot. They have stable yields, good fiber quality and can contribute to a sustainable cotton disease management in tropical environments.
DOI: 10.6084/m9.figshare.14328672
title: Reduced inbreeding depression in separate generations of onions

description: Abstract Mitigating reductions in vigor in onion seed production has been a great challenge to breeding programs. Therefore, we compared the effects of inbreeding depression among populations obtained from self-fertilization and crossbreeding with two and three plants. We evaluated eleven populations and three breeding methods (two and three-plant crossbreeding, and self-fertilization of one plant) and measured the characteristics related to seed yield (plant appearance, stem vigor, number of umbels, flowering uniformity, fruit setting, and resistance to Alternaria) from 2014 to 2015. Thirteen RAPD molecular markers were used to determine the genetic divergence among populations derived from different breeding methods. We found that inbreeding depression affects plant vigor in early generations that have greater heterozygosity. Three-plant crossbreeding results in greater seed yield in populations with low levels of inbreeding. The populations obtained from self-fertilization and two- and three-plant crossbreeding were genetically similar, demonstrating the efficiency of obtaining inbred lines without genetic divergence.
DOI: 10.6084/m9.figshare.14328672.v1
title: Reduced inbreeding depression in separate generations of onions

description: Abstract Mitigating reductions in vigor in onion seed production has been a great challenge to breeding programs. Therefore, we compared the effects of inbreeding depression among populations obtained from self-fertilization and crossbreeding with two and three plants. We evaluated eleven populations and three breeding methods (two and three-plant crossbreeding, and self-fertilization of one plant) and measured the characteristics related to seed yield (plant appearance, stem vigor, number of umbels, flowering uniformity, fruit setting, and resistance to Alternaria) from 2014 to 2015. Thirteen RAPD molecular markers were used to determine the genetic divergence among populations derived from different breeding methods. We found that inbreeding depression affects plant vigor in early generations that have greater heterozygosity. Three-plant crossbreeding results in greater seed yield in populations with low levels of inbreeding. The populations obtained from self-fertilization and two- and three-plant crossbreeding were genetically similar, demonstrating the efficiency of obtaining inbred lines without genetic divergence.
DOI: 10.6084/m9.figshare.14328676
title: Using additive indices and principal components to select sexual genitors and hybrids of Urochloa decumbens

description: Abstract The selection of sexual genitors in Urochloa decumbens breeding is dependent upon the performance of their progeny simultaneously for several traits. Thus, our objectives were to (i) compare the efficiency of indices to select genitors of U. decumbens, (ii) evaluate the genetic gains obtained through selection intensities, and (iii) evaluate the multivariate pattern of progenies through principal components analysis (PCA). For this purpose, 1415 hybrids from 75 progenies of full siblings were evaluated at Embrapa Beef Cattle (Brazil) using seven cuts for dry matter production, regrowth, protein, fiber, lignin, and percentage digestibility. Statistical analyses were performed using mixed models and PCA. The direct selection for dry matter production provided a 37.51% genetic gain. Agronomic traits using indices provided greater gains. Genitors selected using PCA Biplot were similar to selections using indices. Indices and PCA were proven to be an excellent tool to select multi-traits in U. decumbens.
DOI: 10.6084/m9.figshare.14328678
title: Is the genetic variability of elite rice in southern Brazil really disappearing?

description: Abstract There is a worldwide concern about a possible narrowing of the genetic base of most crops, as e.g. that of rice (Oryza sativa L.), as a result of the modern breeding practices. Thus, the purpose of this study was to investigate this phenomenon in the germplasm of elite paddy rice in southern Brazil, including frequently used accessions in crosses. The panel consisted of 91 accessions. Data of morphological traits, SNP markers and mineral content of husked and polished grain were analyzed by hierarchical clustering and principal component analysis. The SNP markers and hierarchical clustering proved most appropriate to assess the genetic variability. A narrowing of the genetic base of rice was confirmed, although a certain level of genetic variability was still found in the germplasm of elite paddy rice in south Brazilian rice, particularly for grain mineral content.
DOI: 10.6084/m9.figshare.14328678.v1
title: Is the genetic variability of elite rice in southern Brazil really disappearing?

description: Abstract There is a worldwide concern about a possible narrowing of the genetic base of most crops, as e.g. that of rice (Oryza sativa L.), as a result of the modern breeding practices. Thus, the purpose of this study was to investigate this phenomenon in the germplasm of elite paddy rice in southern Brazil, including frequently used accessions in crosses. The panel consisted of 91 accessions. Data of morphological traits, SNP markers and mineral content of husked and polished grain were analyzed by hierarchical clustering and principal component analysis. The SNP markers and hierarchical clustering proved most appropriate to assess the genetic variability. A narrowing of the genetic base of rice was confirmed, although a certain level of genetic variability was still found in the germplasm of elite paddy rice in south Brazilian rice, particularly for grain mineral content.
DOI: 10.6084/m9.figshare.14328679
title: Breeding strategies for tropical maize targeting in vivo haploid inducers

description: Abstract: The objective of this study was to compare the selection of plants bred by different pedigree methods using selection among, among and within and only within families. The haploid induction rate of 14 S0:1 and seven S2:3 families, all crossed with the single-cross hybrid GNZ9501, was evaluated. An experimental area of the Department of Biology of the Federal University of Lavras (UFLA), in Lavras, Minas Gerais, in the growing seasons 2012/2013 and 2014/2015, was used for the experiments. In each growing season, one experiment per was carried out, arranged in a complete randomized design, with one and two replications, respectively. Haploid induction was most effective in the families 2 and 6 in both growing seasons. Selection among and within families resulted in higher genetic gains for haploid induction. The results indicated a high genetic variability for haploid induction rate in plants within families.
DOI: 10.6084/m9.figshare.14328679.v1
title: Breeding strategies for tropical maize targeting in vivo haploid inducers

description: Abstract: The objective of this study was to compare the selection of plants bred by different pedigree methods using selection among, among and within and only within families. The haploid induction rate of 14 S0:1 and seven S2:3 families, all crossed with the single-cross hybrid GNZ9501, was evaluated. An experimental area of the Department of Biology of the Federal University of Lavras (UFLA), in Lavras, Minas Gerais, in the growing seasons 2012/2013 and 2014/2015, was used for the experiments. In each growing season, one experiment per was carried out, arranged in a complete randomized design, with one and two replications, respectively. Haploid induction was most effective in the families 2 and 6 in both growing seasons. Selection among and within families resulted in higher genetic gains for haploid induction. The results indicated a high genetic variability for haploid induction rate in plants within families.
DOI: 10.6084/m9.figshare.14328681
title: Adaptability and yield stability of soybean genotypes by REML/BLUP and GGE Biplot

description: Abstract: The aim of this study was to ascertain the association between the REML/BLUP and GGE Biplot methodologies for selection of superior genotypes in regard to adaptability and yield stability for various regions of the Middle North region of Brazil. Sixteen soybean genotypes were evaluated in eight environments during the 2015/2016 and 2016/2017 crop seasons, analyzing the following traits: number of days to maturity, plant height, one hundred seed weight, and grain yield. In this study, the REML/BLUP and the GGE Biplot methods are highly correlated in terms of genotype ranking for selection and recommendation purposes. The genotypes BRASBT13-0528, M8372 IPRO, and BRASBT13-0621 most approximate a hypothetical ideal genotype.
DOI: 10.6084/m9.figshare.14328681.v1
title: Adaptability and yield stability of soybean genotypes by REML/BLUP and GGE Biplot

description: Abstract: The aim of this study was to ascertain the association between the REML/BLUP and GGE Biplot methodologies for selection of superior genotypes in regard to adaptability and yield stability for various regions of the Middle North region of Brazil. Sixteen soybean genotypes were evaluated in eight environments during the 2015/2016 and 2016/2017 crop seasons, analyzing the following traits: number of days to maturity, plant height, one hundred seed weight, and grain yield. In this study, the REML/BLUP and the GGE Biplot methods are highly correlated in terms of genotype ranking for selection and recommendation purposes. The genotypes BRASBT13-0528, M8372 IPRO, and BRASBT13-0621 most approximate a hypothetical ideal genotype.
DOI: 10.6084/m9.figshare.14328682
title: Auxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco

description: Abstract Determining the copy number is important because it can greatly influence the expression level and genetic stability of transgenes. This study aimed to establish a methodology that can estimate the transgene copy number in tobacco (Nicotiana tabacum) using the Auxin independent1 (Axi1) gene as an endogenous control. Tobacco c.v. Petit Havana plants were transformed via Agrobacterium tumefaciens with a pMCG1005 vector that contained Bar as a selective gene. Oligonucleotide efficiency was determined and a qPCR using the 2-ΔCt and 2-ΔΔCt methods was performed. Bar was the target gene and Axi1 was the endogenous control in five transgenic tobacco events. The results showed that the Axi1 gene was donated by the maternal parent N. sylvestris when interspecific hybridization occurred between N. sylvestris and N. tomentosiformis. The copy number results agreed with the segregation ratios for the Bar gene in T1 plants, which confirmed that Axi1 is a single copy gene that can be used as an endogenous control.
DOI: 10.6084/m9.figshare.14328682.v1
title: Auxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco

description: Abstract Determining the copy number is important because it can greatly influence the expression level and genetic stability of transgenes. This study aimed to establish a methodology that can estimate the transgene copy number in tobacco (Nicotiana tabacum) using the Auxin independent1 (Axi1) gene as an endogenous control. Tobacco c.v. Petit Havana plants were transformed via Agrobacterium tumefaciens with a pMCG1005 vector that contained Bar as a selective gene. Oligonucleotide efficiency was determined and a qPCR using the 2-ΔCt and 2-ΔΔCt methods was performed. Bar was the target gene and Axi1 was the endogenous control in five transgenic tobacco events. The results showed that the Axi1 gene was donated by the maternal parent N. sylvestris when interspecific hybridization occurred between N. sylvestris and N. tomentosiformis. The copy number results agreed with the segregation ratios for the Bar gene in T1 plants, which confirmed that Axi1 is a single copy gene that can be used as an endogenous control.
DOI: 10.6084/m9.figshare.14328684
title: A half century of a bean breeding program in the South and Alto Paranaíba regions of Minas Gerais

description: Abstract The success of bean (Phaseolus vulgaris L.) crops in Brazil is due to the development of new cultivars. The aim of the present study was to critically analyze the fifty years of a bean breeding program at the Universidade Federal de Lavras (UFLA) and estimate the genetic progress (GP) achieved for grain yield. The estimated genetic progress for grain yield was 0.35% per year, which is associated with visible improvement in plant architecture, resistance to some pathogens, and better carioca bean grain quality. The program was found to be efficient, generating 119 theses and 43 dissertations during the period under study. In addition, hundreds of articles have been published, especially studies aimed at improving the efficiency of genetic breeding programs. Over the past few years, partnerships with other public institutions in performing Value for Cultivation and Use tests have resulted in 12 recommended cultivars.