DOI: 10.22002/kh5cc-c2p18
title: Caulobacter crescentus

description: wild type CB15N. 2 of 4, too warped for reconstructing. no reconstruction generated because fine alignment shows that ice warped too much between tilt halves
description: <strong>Tilt Series Date:</strong> 2006-08-15</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> </p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; 7 days growth; see DPD notebook Aug 07-14, 2006</p>
<strong>Sample Preparation:</strong> box 705, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-08-15-1/rawdata/</p>CC06Aug15e200t01.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-08-15-1/rawdata/CC06Aug15e200t01.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC06Aug15e200t01b.mrc,  Tilt Series (Pixel Size 1.2 nm), 637.7 MB  
        
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        grid1_220X_xm434y324.dm3,  * associated to rawdata, 8.6 MB  
        
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        grid2_220X_x127y251.dm3,  * associated to rawdata, 8.6 MB  
        
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        grid2_220X_xm244y242.dm3,  * associated to rawdata, 8.6 MB  
        
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        SAVE_06Aug15t01_13kx..dm3,  8.6 MB  
        
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        SAVE_grid1_220X_xm434y324.dm3,  8.6 MB  
        
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        SAVE_grid2_220X_x127y251.dm3,  8.6 MB  
        
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        SAVE_grid2_220X_xm244y242.dm3,  8.6 MB  
        
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