cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.b42s4
title: Data from: Ecdysteroid hormones link the juvenile environment to alternative adult life histories in a seasonal insect

description: The conditional expression of alternative life strategies is a widespread
 feature of animal life and a pivotal adaptation to life in seasonal
 environments. To optimally match suites of traits to seasonally changing
 ecological opportunities, animals living in seasonal environments need
 mechanisms linking information on environmental quality to resource
 allocation decisions. The butterfly Bicyclus anynana expresses alternative
 adult life histories in the alternating wet and dry seasons of its habitat
 as endpoints of divergent developmental pathways triggered by seasonal
 variation in preadult temperature. Pupal ecdysteroid hormone titers are
 correlated with the seasonal environment, but whether they play a
 functional role in coordinating the coupling of adult traits in the
 alternative life histories is unknown. Here, we show that manipulating
 pupal ecdysteroid levels is sufficient to mimic in direction and magnitude
 the shifts in adult reproductive resource allocation normally induced by
 seasonal temperature. Crucially, this allocation shift is accompanied by
 changes in ecologically relevant traits, including timing of reproduction,
 life span, and starvation resistance. Together, our results support a
 functional role for ecdysteroids during development in mediating strategic
 reproductive investment decisions in response to predictive indicators of
 environmental quality. This study provides a physiological mechanism for
 adaptive developmental plasticity, allowing organisms to cope with
 variable environments.
description: Four datasets from
 Oostra et al. Am Nat 2014Four datasets from:
 Ecdysteroid hormones link the juvenile environment to alternative adult
 life histories in a seasonal insect; by Oostra, Vicencio; Mateus, Ana Rita
 A.; Van der Burg, Karin R.L.; Piessens,Thomas; Van Eijk, Marleen;
 Brakefield, Paul M.; Beldade, Patrícia; Zwaan, Bas; as published in
 American Naturalist 2014. This ZIP archive contains four csv data files:
 1) experiment1.csv; 2) experiment2_dataset1.csv; 3)
 experiment2_dataset2.csv; 4) experiment2_dataset3.csv. See readme.txt for
 details.data_from_Oostra_et_al_Am_Nat_2014.zip
DOI: 10.15468/dl.z5yhmq
title: Occurrence Download

description: A dataset containing 45 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aosa grandis",
 "HasCoordinate is true",
 "BasisOfRecord is one of (Specimen)"
 ]
}

 The dataset includes 45 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001066-260126135527185/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5061/dryad.63t28
title: Data from: Spatial analysis of gene regulation reveals new insights into the molecular basis of upper thermal limits

description: The cellular stress response has long been the primary model for studying
 the molecular basis of thermal adaptation, yet the link between gene
 expression, RNA metabolism and physiological responses to thermal stress
 remains largely unexplored. We address this by comparing the
 transcriptional and physiological responses of three geographically
 distinct populations of D. melanogaster from eastern Australia in response
 to, and recovery from, a severe heat stress with and without a pre-stress
 hardening treatment. We focus on Starvin (stv), recently identified as an
 important thermally responsive gene. Intriguingly, stv encodes seven
 transcripts from alternative transcription sites and alternative splicing,
 yet appears to be rapidly heat-inducible. First, we show genetic
 differences in upper thermal limits of the populations tested. We then
 demonstrate that the stv locus does not ubiquitously respond to thermal
 stress but is expressed as three distinct thermal and temporal RNA
 phenotypes (isoforms). The shorter transcript isoforms are rapidly
 up-regulated under stress in all populations and show similar molecular
 signatures to Heat shock proteins. Multiple stress exposures seem to
 generate a reserve of pre-mRNAs, effectively ‘priming’ the cells for
 subsequent stress. Remarkably, we demonstrate a bypass in the splicing
 blockade in these isoforms, suggesting an essential role for these
 transcripts under heat stress. Temporal profiles for the weakly heat
 responsive stv isoform subset show opposing patterns in the two most
 divergent populations. Innate and induced transcriptome responses to
 hyperthermia are complex, and warrant moving beyond gene-level analyses.
description: Data from: Spatial
 analysis of gene regulation reveals new insights into the molecular basis
 of upper thermal limits.This compressed folder
 contains three data files comprising the raw phenotype data (heat
 knockdown), the raw real time PCR data for the stv isoforms and reference
 gene RPL11, and the normalised real-time PCR data for the stv
 isoformsData_MEC-14-0904.R1.zip
DOI: 10.5061/dryad.jd5j3
title: Data from: Ontogenetic changes in the phenotypic integration and modularity of leaf functional traits

description: 1.Changes in resource availability, functional demands, hormonal
 regulation and developmental constraints can promote differences in the
 expression of leaf traits during plant development and foster changes in
 the targets of natural selection. As a consequence, the pattern and
 magnitude of covariation among traits, and therefore their phenotypic
 integration and modularity are equally expected to change throughout
 ontogeny. However, these changes have not been described yet. 2.We
 measured leaf economic, defensive and morphological traits in plants of
 Turnera velutina and estimated the magnitude and pattern of foliar
 integration and modularity for juvenile and reproductive individuals. In
 addition, we assessed the relationship between plant biomass and foliar
 integration within and among ontogenetic stages. 3.Both the pattern and
 magnitude of foliar integration changed across plant ontogeny. Foliar
 integration was lower in juvenile than in reproductive plants, and the
 pattern of phenotypic integration and modularity was different between
 ontogenetic stages: whereas leaves from juvenile plants showed two
 functional modules related to plant defence and leaf economy, traits from
 reproductive plants had greater interconnectivity and hence lower
 modularity. 4.The relationship between plant biomass and foliar
 integration was negative within each ontogenetic stage but positive
 between ontogenetic stages, suggesting that processes intrinsic to plant
 development influenced the magnitude of foliar integration to a greater
 extent than plant size. 5.Our findings indicate that plants can change the
 patterns of covariation among leaf traits during their development.
 Whereas a lower foliar integration in juvenile plants could allow for
 greater lability to explore a multi-trait phenotypic space, canalization
 of leaf attributes along ontogeny should promote greater phenotypic
 integration, constraining the number of multi-trait combinations that
 plants can express. Hence, we suggest that ontogenetic changes in foliar
 integration allow plants to deal with changing selective dynamics and
 physiological priorities along their development.
description: foliar traits in
 turneraDataset of foliar traits
 used to estimate phenotypic integration of 24 genotypes in juvenile and
 reproductive plants of Turnera velutina.phenotypic_integration_turnera_leaves.xlsx
DOI: 10.5061/dryad.4sd09
title: Data from: Asteraceae pollen provisions protect Osmia mason bees (Hymenoptera: Megachilidae) from brood parasitism

description: Many specialist herbivores eat foods that are apparently low quality. The
 compensatory benefits of a poor diet may include protection from natural
 enemies. Several bee lineages specialize on pollen of the plant family
 Asteraceae, which is known to be a poor-quality food. Here we tested the
 hypothesis that specialization on Asteraceae pollen protects bees from
 parasitism. We compared rates of brood parasitism by Sapyga wasps on
 Asteraceae-specialist, Fabeae-specialist, and other species of Osmia bees
 in the field over several years and sites and found that
 Asteraceae-specialist species were parasitized significantly less
 frequently than other species. We then tested the effect of Asteraceae
 pollen on parasites by raising Sapyga larvae on three pollen mixtures:
 Asteraceae, Fabeae, and generalist (a mix of primarily non-Asteraceae
 pollens). Survival of parasite larvae was significantly reduced on
 Asteraceae provisions. Our results suggest that specialization on
 low-quality pollen may evolve because it helps protect bees from natural
 enemies.
description: sapygid_parasitism_2008-2015Incidence of parasitism by sapygid wasps on species of Osmia using different types of pollen, based on trapnests established at multiple field sites between 2008 and 2015. Please consult the ReadMe file for details.sapygid_pollen_experimentMany specialist herbivores eat foods that are apparently "low-quality". The compensatory benefits of a poor diet may include protection from natural enemies. Several bee lineages specialize on pollen of the plant family Asteraceae, which is known to be a poor-quality food. Here we tested the hypothesis that specialization on Asteraceae pollen protects bees from parasitism. We compared rates of brood parasitism by Sapyga wasps on Asteraceae-specialist, Fabeae-specialist, and other species of Osmia bees in the field over several years and sites, and found that Asteraceae-specialist species were parasitized significantly less frequently than other species. We then tested the effect of Asteraceae pollen on parasites by raising Sapyga larvae on three pollen mixtures: Asteraceae, Fabeae, and generalist (a mix of primarily non-Asteraceae pollens). Survival of parasite larvae was significantly reduced on Asteraceae provisions. Our results suggest that specialization on low-quality pollen may evolve because it helps protect bees from natural enemies.
DOI: 10.5061/dryad.0vr6m66t
title: Data from: Geographic structure and adaptive population differentiation in herbivore defense genes in European aspen (Populus tremula L., Salicaceae)

description: When a phenotypic trait is subjected to spatially variable selection and
 local adaptation, the underlying genes controlling the trait are also
 expected to show strong patterns of genetic differentiation because
 alternative alleles are favoured in different geographical locations.
 Here, we study 71 single nucleotide polymorphisms (SNPs) from seven genes
 associated with inducible defence responses in a sample of Populus tremula
 collected from across Sweden. Four of these genes (PPO2, TI2, TI4 and TI5)
 show substantial population differentiation, and a principal component
 analyses conducted on the defence SNPs divides the Swedish population into
 three distinct clusters. Several defence SNPs show latitudinal clines,
 although these were not robust to multiple testing. However, five SNPs
 (located within TI4 and TI5) show strong longitudinal clines that remain
 significant after multiple test correction. Genetic geographical
 variation, supporting local adaptation, has earlier been confirmed in
 genes involved in the photoperiod pathway in P. tremula, but this is, to
 our knowledge, one of the first times that geographical variation has been
 found in genes involved in plant defence against antagonists.
description: control_SNPsdata file containing 93 control SNPs (SNPs without any correlation to defense mechanisms) scored in 115 individuals from the SwAsp collection of European aspen (Populus tremula)defense_SNPsdata file containing 71 defense SNPs scored in 115 individuals from the SwAsp collection of European aspen (Populus tremula)patterson.RR scrips file for calculating population structure using the principle component analysis method of Patterson et al. (2006)TW tableCumulative distribution of Tracy-Widom statistic used in calculating significance of population structure using PCA method of Patterson et al (2006).twtable
DOI: 10.5061/dryad.tg386p7
title: Data from: A competitive environment influences sperm production, but not testes tissue composition, in house mice

description: Due to the physiological cost of sperm production, males are expected to
 be prudent in their expenditure and adjust their investment according to
 current social conditions. Strategic adjustments in sperm expenditure
 during development can be made via changes in testes size, sperm
 production rates, or testes tissue composition. Here, using house mice, we
 test the hypothesis that elevated sperm production is driven by a plastic
 response in the spatial organisation of the testes. We reared males under
 different social conditions (competitive vs. non-competitive) and
 quantified sperm number and the proportion of sperm‐producing tissue
 within the testes. Further, because sperm quality is a critical
 determinant of competitive fertilization success, we used
 computer-assisted sperm analysis to quantify six sperm motility traits.
 Our investigation revealed that males reared in an environment with a
 perceived risk of reproductive competition produced more sperm in the
 absence of changes in testes morphology. We discuss this result in
 relation to fixed and flexible phenotypically plastic responses to future
 competitive conditions, and conclude that adaptive adjustments in sperm
 number in response to the social environment are likely attributable to
 variation in sperm production rate. Further, we found no difference in in
 vitro sperm motility parameters among males from the different social
 environment regimes. Overall, this investigation improves our
 understanding of the mechanisms of male plastic responses to reproductive
 competition experienced during sexual development.
description: Firman et al. JEB
 2018
DOI: 10.5061/dryad.2p00v
title: Data from: Measuring biomass and carbon stock in resprouting woody plants

description: Resprouting multi-stemmed woody plants form an important component of the
 woody vegetation in many ecosystems, but a clear methodology for reliable
 measurement of their size and quick, non-destructive estimation of their
 woody biomass and carbon stock is lacking. Our goal was to find a minimum
 number of sprouts, i.e., the most easily obtainable, and sprout parameters
 that should be measured for accurate sprout biomass and carbon stock
 estimates. Using data for 5 common temperate woody species, we modelled
 carbon stock and sprout biomass as a function of an increasing number of
 sprouts in an interaction with different sprout parameters. The mean basal
 diameter of only two to five of the thickest sprouts and the basal
 diameter and DBH of the thickest sprouts per stump proved to be accurate
 estimators for the total sprout biomass of the individual resprouters and
 the populations of resprouters, respectively. Carbon stock estimates were
 strongly correlated with biomass estimates, but relative carbon content
 varied among species. Our study demonstrated that the size of the
 resprouters can be easily measured, and their biomass and carbon stock
 estimated; therefore, resprouters can be simply incorporated into studies
 of woody vegetation.
description: Data_biomass, carbon and
 enery in sprouts with averagege sprout parameters of 10 largest
 sproutsData for whole
 resprouting woody plants. They include sprout biomass weight, carbon
 content, average sprout parameters for 1-10 largest sprouts - basal
 diameter (BD), diameter at breast height (DBH) and height
 (H).Data for individual
 sprouts_size and weightData on individual
 sprouts of 5 common temperate woody species. They include sprout weight
 and sprout parameters - length, basal diameter and diameter at breast
 height (DBH) - for 3 sprouts per plant: large (A), medium (B) and small
 (C) (within resprouting woody plant identified by ID).
DOI: 10.5061/dryad.62v82
title: Data from: No evidence for induction or selection of mutant sodium channel expression in the copepod Acartia husdsonica challenged with the toxic dinoflagellate Alexandrium fundyense

description: Some species in the dinoflagellate genus Alexandrium spp. produce a suite
 of neurotoxins that block sodium channels, known as paralytic shellfish
 toxins (PST), which have deleterious effects on grazers. Populations of
 the ubiquitous copepod grazer Acartia hudsonica that have co-occurred with
 toxic Alexandrium spp. are better adapted than naïve populations. The
 mechanism of adaptation is currently unknown. We hypothesized that a
 mutation in the sodium channel could account for the grazer adaptation. We
 tested two hypotheses: (1) Expression of the mutant sodium channel could
 be induced by exposure to toxic Alexandrium fundyense; (2) in the absence
 of induction, selection exerted by toxic A. fundyense would favor copepods
 that predominantly express the mutant isoform. In the copepod A.
 hudsonica, both isoforms are expressed in all individuals in varying
 proportions. Thus, in addition to comparing expression ratios of wild-type
 to mutant isoforms for individual copepods, we also partitioned copepods
 into three groups: those that predominantly express the mutant (PMI)
 isoform, the wild-type (PWI) isoform, or both isoforms approximately
 equally (EI). There were no differences in isoform expression between
 individuals that were fed toxic and nontoxic food after three and 6 days;
 induction of mutant isoform expression did not occur. Furthermore, the
 hypothesis that mutant isoform expression responds to toxic food was also
 rejected. That is, no consistent evidence showed that the wild-type to
 mutant isoform ratios decreased, or that the relative proportion of PMI
 individuals increased, due to the consumption of toxic food over four
 generations. However, in the selected line that was continuously exposed
 to toxic food sources, egg production rate increased, which suggested that
 adaptation occurred but was unrelated to sodium channel isoform
 expression.
description: Finiguerra et al., 2014,
 Ecology and Evolution, Data SharingThis file contains all
 data used for plotting and statistical analyses.
DOI: 10.5061/dryad.44mt9
title: Data from: Are pollinators the agents of selection on flower colour and size in irises?

description: Plant-pollinator interactions are believed to play a major role in the
 evolution of floral traits. Flower colour and flower size are important
 for attracting pollinators, directly influencing reproduction, and thus
 expected to be under pollinator-mediated selection. Pollinator-mediated
 selection is also proposed to play a role in maintaining flower colour
 polymorphism within populations. However, pigment concentrations, and thus
 flower colour, are also under selective pressures independent of
 pollinators. We quantified phenotypic pollinator-mediated selection on
 flower colour and size in two colour polymorphic Iris species. Using
 female fitness, we estimated phenotypic selection on flower colour and
 size, and tested for pollinator-mediated selection by comparing selection
 gradients between flowers open to natural pollination and supplementary
 pollinated flowers. In both species, we found evidence for pollen
 limitation, which set the base for pollinator-mediated selection. In the
 colour dimorphic Iris lutescens, while pigment concentration and flower
 size were found to be under selection, this was independent of
 pollinators. For the polymorphic Iris pumila, pigment concentration is
 under selective pressure by pollinators, but only for one colour morph.
 Our results suggest that pollinators are not the main agents of selection
 on floral traits in these irises, as opposed to the accepted paradigm on
 floral evolution. This study provides an opposing example to the
 largely-accepted theory that pollinators are the major agent of selection
 on floral traits.
description: Data for both
 speciesComplete dataset used in
 this articlephenotypes.ods
DOI: 10.5061/dryad.406248v
title: Data from: Sexual attractiveness and reproductive performance in aging females of a coccoid insect

description: Female age strongly influences reproductive success in various animals.
 Males are therefore expected to show preferential responses to sexual
 signals released from females of ages suitable for reproduction. Here,
 however, we report an unexpected and contradictory effect of aging on
 sexual attractiveness and reproductive performance in a coccoid insect:
 the pheromone-based attractiveness of females increased with aging, though
 their reproductive performance was in rapid decline. Surprisingly,
 senescent females continued releasing relatively high amounts of pheromone
 and maintained their sexual attractiveness, even at ages when they had
 almost completely lost fertility, with reduced densities of endosymbionts
 to support their physiology. Our dataset suggests a potential sexual
 conflict within a pheromone communication system, where females benefit at
 males’ expense through deceptive signals of fertility.
description: Tabata_MealybugAging_20180523
DOI: 10.5061/dryad.2hb12
title: Data from: Differential population responses of native and alien rodents to an invasive predator, habitat alteration, and plant masting

description: Invasive species and anthropogenic habitat alteration are major drivers of
 biodiversity loss. When multiple invasive species occupy different trophic
 levels, removing an invasive predator might cause unexpected outcomes due
 to complex interactions among native and non-native prey. Moreover,
 external factors such as habitat alteration and resource availability can
 affect such dynamics. We hypothesized that native and non-native prey
 respond differently to an invasive predator, habitat alteration, and
 bottom–up effects. To test the hypothesis, we used Bayesian state-space
 modelling to analyse 8-year data on the spatio-temporal patterns of two
 endemic rat species and the non-native black rat in response to the
 continual removal of the invasive small Indian mongoose on Amami Island,
 Japan. Despite low reproductive potentials, the endemic rats recovered
 better after mongoose removal than did the black rat. The endemic species
 appeared to be vulnerable to predation by mongooses, whose eradication
 increased the abundances of the endemic rats, but not of the black rat.
 Habitat alteration increased the black rat's carrying capacity, but
 decreased those of the endemic species. We propose that spatio-temporal
 monitoring data from eradication programs will clarify the underlying
 ecological impacts of land-use change and invasive species, and will be
 useful for future habitat management.
description: Fukasawa et al.
 datasetThis zip file contains a
 dataset file (fukasawa2013data.csv) used to estimate the Bayesian
 state-space model, and a Shapefile (a set of 5 files named
 "fukasawa2013grid.***") which includes polygon data of the
 dataset location.fukasawa2013.zip