cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/b89gv-p5g02
title: JV7091_ΔdotEP

description: JV7091_ΔdotEP (Deletion of dotE and dotP)
description: <strong>Tilt Series Date:</strong> 2017-10-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~2.8</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain at OD~ 2.8, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-8/rawdata/</p>JV7091-201710300008.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-8/rawdata/JV7091-201710300008.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV7091-201710300008_part61_8.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-8/3dimage_89896/JV7091-201710300008_part61_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.y5gr72
title: Occurrence Download

description: A dataset containing 83679 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Asperula cynanchica",
 "Year 1954-2022"
 ]
}

 The dataset includes 83679 records from 818 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006549-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/f296h-qrz83
title: JV3559_Janelia

description: Legionella thyA ΔdotG.This is a single mutant that lacks DotG.
description: <strong>Tilt Series Date:</strong> 2017-01-08</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~3.4</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the JV3559 strain at OD~ 3.4, 4 grids at density x and another 4 at density 2x. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-08-17/rawdata/</p>JV3559_20170106_area10054.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-08-17/rawdata/JV3559_20170106_area10054.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV3559_20170106_area10054_full.rec,  Reconstruction (Pixel Size 1.68 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-08-17/3dimage_80279/JV3559_20170106_area10054_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nhdcf-rhe38
title: JV4404_delDot.Icm_Lp02

description: JV4404 is deletion of the Dot.Icm system. Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0
description: <strong>Tilt Series Date:</strong> 2015-12-11</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor, also manually processed 12 tomograms (and used SIRT) to check for presence of the T4SS basal-body</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. This data was collected at the Caltech Polara.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. Added gold before freezing.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen for JV4404 at OD= 2.9 Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-12-11-13/3dimage_71344/</p>JV44040012_part111_1.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-12-11-13/3dimage_71344/JV44040012_part111_1.rec"
        > <i class="download icon"></i> Download </a></p>

        JV44040012.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-12-11-13/rawdata/JV44040012.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4j99n-ns656
title: Vibrio cholerae SAD2095

description: SAD2095 (T2SS and T4 competence pili)
description: <strong>Tilt Series Date:</strong> 2019-09-05</p>
<strong>Data Taken By:</strong> Sara Weaver</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> SAD2095</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, -60.0°), step: 1.0°, constant angular increment, dosage: 134.0 eV/Å²,  defocus: -4.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ankur B Dalia ankdalia@indiana.edu, PhD, Assistant Professor, Indiana University. Cells grown, samples prepared by Andrew Schacht, data collected by Sara Weaver, Strain from Ankur B Dalia ankdalia@indiana.edu, PhD, Assistant Professor, Indiana University. Cells grown, data collected by Sara Weaver, and Matthew Sazinsky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 2019/06/05: Grew up a single colony in 3 mL LB with shaking at 37?C. The next day, subcultured 50uL of this overnight culture into 3mL of fresh LB + 100uM IPTG + 20mM MgCl2 + 10mM CaCl2 and grow at 30?C shaking for ~8 hours. This induced expression of the transcription factor TfoX to turn on the T2SS Type IV competence pilus.</p>
<strong>Sample Preparation:</strong> 2019/06/05 spun gold (100 uL 10 nm gold + 25 uL 5% BSA) at 15,000rpm for 20 minutes, removed supernatant. Resuspended gold beads in 300 uL liquid culture that was washed in 7 g/L instant ocean, resuspended in 50 uL instant ocean.  40 uL was then aliquoted into the gold pellet Vitrobot 4 deg C and 100% humidity, 3uL of gold/sample slurry on carbon/copper, extra thick carbon, Quantifoil Finder grids R2/2 (EmiTech glow discharge 15 mA 60 s) Blot force +2, blot time 3 s, no waiting time, no drain time. Plunged into ethane/propane mix.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2019-09-05-19/rawdata/</p>20190905_ASBox2Grid2_TS17-half1.mrc,  Tilt Series (Pixel Size 0.336 nm), 2.9 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        20190905_ASBox2Grid2_TS17-half1_full.rec,  Reconstruction (Pixel Size 1.344 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swj2019-09-05-19/3dimage_106103/20190905_ASBox2Grid2_TS17-half1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w7pyn-0tr39
title: JV5443

description: JV5443 Legionella (Super-deletion+CDHFG), no Thy
description: <strong>Tilt Series Date:</strong> 2016-08-09</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate and cysteine hydrochloride  (no thymidine). Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0.</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the JV5443 strain at OD~3.2. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2016-08-09-16_slicer16935.jpg: Why two Legionella cells have different peliplasmic space volume? Have seen this before also. Two different cell stages/forms? dga2016-08-09-16_slicer16936.jpg: Why two Legionella cells have different peliplasmic space volume? Have seen this before also. Two different cell stages/forms?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-16/rawdata/</p>JV5443a0068.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-16/rawdata/JV5443a0068.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV5443a0068_full.rec,  Reconstruction (Pixel Size 1.68 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-16/3dimage_77606/JV5443a0068_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8mchkc
title: Occurrence Download

description: A dataset containing 65126 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ichthyaetus melanocephalus",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Geometry POLYGON ((-6.6 29.26705, -6.6 50.94, 40.81373 50.94, 40.81373 29.26705, -6.6 29.26705))",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Observation, Material sample)"
 ]
}

 The dataset includes 65126 records from 24 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006501-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/hgz1k-kwd90
title: Agro_AD1526_GFP-VirB11_20150826

description: Agro_AD1526_GFP-VirB11 (Cb+Kan+AS plates, Mes pH5.8). Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1526) and wash the plate by gently rolling the liquid. Take this liquid and add to the other plate. Repeat the same. This should get enough cells dissolved in the liquid.Add gold to this: 1ul of gold to 4 ul of cells.
description: <strong>Tilt Series Date:</strong> 2015-08-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow AD1526 cells on AB solid medium (pH 7) at 30C. For induction, use one single colony and spread on induction plates (AB Mes pH 5.8) containing  Cb (50ug/ml)+Kan (50ug/ml)+AS (200µM). Grow at 19C for 48 hr. Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1526) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  dga2015-08-26-46_slicer14360.jpg: Two cells connected by a tube, similar to conjugation? dga2015-08-26-46_slicer14361.jpg: Two cells connected by a tube, similar to conjugation? dga2015-08-26-46_slicer14362.jpg: Two cells connected by a tube, similar to conjugation? dga2015-08-26-46_slicer14363.jpg: Two cells connected by a tube, similar to conjugation?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-46/3dimage_60852/</p>AD1526tomo0026_part61_1.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-46/3dimage_60852/AD1526tomo0026_part61_1.rec"
        > <i class="download icon"></i> Download </a></p>

        AD1526tomo0026.mrc,  Tilt Series (Pixel Size 0.42 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-46/rawdata/AD1526tomo0026.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.34740/kaggle/dsv/12903578
title: World Weather Repository ( Daily Updating )
DOI: 10.15468/dl.fgr26j
title: Occurrence Download

description: A dataset containing 1231 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is Cambodia",
 "OccurrenceStatus is Present",
 "TaxonKey is Mulleripicus pulverulentus"
 ]
}

 The dataset includes 1231 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006587-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bkgnq-8kh55
title: H.pylori 26695 delta-cagH / AGS

description: grid box: YWC080
description: <strong>Tilt Series Date:</strong> 2014-02-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori & AGS cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-02-14-6_slicer3678.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Two tubes visible.  Possibly T4SS related. ycw2014-02-14-6_slicer3679.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Four tubes visible.  Possibly T4SS related. ycw2014-02-14-6_slicer3681.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Tube projecting from Hp cell. Possibly T4SS related. ycw2014-02-14-6_slicer3682.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Three tubes, including one super-tube over 1 um long, projecting from Hp cell. Possibly T4SS related. ycw2014-02-14-6_slicer3683.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Super-tube. Possibly T4SS related.
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-6/rawdata/</p>20140214_YWC080_10003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-6/rawdata/20140214_YWC080_10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140214_YWC080_10003_part121_8.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6ataj-1nd74
title: Rhodobacter 1558 lyse ficoll

description: Rhodobacter sphaeroides JPA 1558 lysed with crowding agents
description: <strong>Tilt Series Date:</strong> 2013-09-26</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Spun down for 10' at 5100 rpm. Resuspended pellets in 5 mL PEG-Trehalose-Ficoll-Sucrose-Tris-HCl buffer (7.5% PEG 8000, 4% Trehalose, 20% Sucrose, 25 mM Tris-HCl pH 7.0,10% Ficoll)    * 2.14 mL PEG 8000   * 1 mL Trehalose   * 2 g Sucrose   * 1 g Ficoll   * Q.S. 10 mL 25 mM Tris-HCl, pH 7.0Note:  Ficoll didn't go into solution after 4 hours, so spun down and used aqueous phase.  In future, try less Ficoll?Add 500 µL of 50 mg/mL lysozyme stock (make fresh, in 10 mM Tris-HCl, pH 8.0) for a final concentration of 5 mg/mL Incubate 15' at room temperature Add 28 µL of magnesium chloride, 3.3 µL of calcium chloride, and 5.5 µL of DNase IIncubate 30' at 37 degrees Celsius Spin down for 10' at 5100 rpm.Resuspend with gentle pipetting in 200 µL K-phosphate-PEG-Trehalose-Sucrose buffer (7.5% PEG-8000, 4% Trehalose, 20% Sucrose, 5 mM K-phosphate buffer)    * 2.14 mL PEG 8000   * 1 mL Trehalose   * 2 g Sucrose   * Q.S. 10 mL 5 mM K-phosphate buffer, pH 7.0 9/17/13:  Seems to work well.  Good spheroplast formation, and intact clusters.Freeze grids in grid box "Waldo Ficoll"</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-09-26-6/rawdata/</p>Waldo22mag160dose0006.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-09-26-6/rawdata/Waldo22mag160dose0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        Waldo22mag160dose0006_part121_4.rec,  Reconstruction (Pixel Size 0.96 nm), 2.9 GB  
        
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        > <i class="download icon"></i> Download </a></p>