cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/3je42-2re36
title: E. coli (pLE7, 20 uM IPTG)

description: Cells contain WT MreB in the chromosome plus an N-terminal YFP fusion to MreB (YFP-MreB) on the plasmid pLe7. pLE7 also confers a resistance to ampicillin.
description: <strong>Tilt Series Date:</strong> 2011-06-02</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> Gift from Larry Rothfield</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot plunge freezing</p>
  mst2011-06-02-24_slicer9081.jpg: unknown filament, Escherichia coli mst2011-06-02-24_slicer9082.jpg: filament bundle, Escherichia coli
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-06-02-24/rawdata/</p>20110602_PLE7_29.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-06-02-24/rawdata/20110602_PLE7_29.mrc"
        > <i class="download icon"></i> Download </a></p>

        20110602_PLE7_29_full.rec,  Reconstruction (Pixel Size 1.9 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-06-02-24/3dimage_19063/20110602_PLE7_29_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y8f2e-rw253
title: Myxococcus xanthus DK1622 wt

description: For T4P averaging
description: <strong>Tilt Series Date:</strong> 2013-08-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-08-26-82_slicer9132.jpg: Myxococcus Xanthus .  unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-82/rawdata/</p>20130826_DK1622_WT_30023.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-82/rawdata/20130826_DK1622_WT_30023.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130826_DK1622_WT_30023_part131_19.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-26-82/3dimage_30278/20130826_DK1622_WT_30023_part131_19.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eb20y-r1h75
title: MT310_xyl(+)_Cd50uM(+)

description: Raw data files of MT310_xyl(+)_Cd50uM(+)
description: <strong>Tilt Series Date:</strong> 2009-06-09</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> The MT310 strain was constructed by Martin Thanbichler; cells were cultured and treated by Zhuo Li; data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were induced by xylose and treated with 50 uM CdCl2 for 20 hours.</p>
<strong>Sample Preparation:</strong> 10 nm gold. </p>
  zla2009-06-09-28_slicer8128.jpg: filament bundle, Caulobacter crescentus zla2009-06-09-28_slicer8130.jpg: flat vesicles, Caulobacter crescentus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-28/rawdata/</p>09jun09a_Cd50uM_047.mrc,  Tilt Series (Pixel Size 0.95 nm), 511.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-28/rawdata/09jun09a_Cd50uM_047.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun09a_Cd50uM_047_full.rec,  Reconstruction (Pixel Size 1.9 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-28/3dimage_5159/09jun09a_Cd50uM_047_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.9q8e8k
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea chamaedryfolia",
 "Country is Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002609-250827131500795/datasets/export for details.
DOI: 10.22002/k3j69-pvz97
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-09-30</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-68.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-76/rawdata/</p>RCC745_G1tovis_245.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-76/rawdata/RCC745_G1tovis_245.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_245.rec,  Reconstruction (Pixel Size 1.26 nm), 61.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-09-30-76/3dimage_12239/RCC745_G1tovis_245.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q0p0v-bf389
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-4/rawdata/</p>RCC745_RELtovis_004.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-4/rawdata/RCC745_RELtovis_004.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_004.rec,  Reconstruction (Pixel Size 1.26 nm), 346.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-4/3dimage_12349/RCC745_RELtovis_004.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wk6cc-bwg38
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-40/3dimage_11691/</p>RCC745_MGtovis_355.rec,  Reconstruction (Pixel Size 1.26 nm), 311.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-40/3dimage_11691/RCC745_MGtovis_355.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_355.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-40/rawdata/RCC745_MGtovis_355.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bnmzg-r8r94
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-88/rawdata/</p>RCC745_RELtovis_097.mrc,  Tilt Series (Pixel Size 1.26 nm), 570.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-88/rawdata/RCC745_RELtovis_097.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_097.rec,  Reconstruction (Pixel Size 1.26 nm), 693.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-88/3dimage_12517/RCC745_RELtovis_097.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/43f4y-6n426
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-11-16</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-13/rawdata/</p>RCC745_MGtovis_409.mrc,  Tilt Series (Pixel Size 1.26 nm), 771.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-13/rawdata/RCC745_MGtovis_409.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_409.rec,  Reconstruction (Pixel Size 1.26 nm), 463.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-11-16-13/3dimage_11791/RCC745_MGtovis_409.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4dd49-tb666
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC114
description: <strong>Tilt Series Date:</strong> 2014-08-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-20/rawdata/</p>20140811_YWC114_30010.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-20/rawdata/20140811_YWC114_30010.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140811_YWC114_30010_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-20/3dimage_45459/20140811_YWC114_30010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xeee1-a7y83
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-26/rawdata/</p>RCC745_RELtovis_029.mrc,  Tilt Series (Pixel Size 1.26 nm), 771.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-26/rawdata/RCC745_RELtovis_029.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_029.rec,  Reconstruction (Pixel Size 1.26 nm), 307.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-26/3dimage_12393/RCC745_RELtovis_029.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/txzmd-cqv06
title: MT310_xyl(+)_metal(+)_A22(+)

description: Raw data files of MT310_xyl(+)_metal(+)_A22(+)
description: <strong>Tilt Series Date:</strong> 2009-06-17</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> MT310 was constructed by Martin Thanbichler. The cell was cultured and treated by Zhuo Li. Data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MT310 cells were induced by xylose and treated with 50 uM AuCl for 20 hours, and treated with 10 ug/ml A22 for 1.5 hours.</p>
<strong>Sample Preparation:</strong> 10 nm gold. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-17-20/rawdata/</p>09jun17a_A22c_025.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-17-20/rawdata/09jun17a_A22c_025.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun17a_A22c_025_full.rec,  Reconstruction (Pixel Size 1.9 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-17-20/3dimage_5018/09jun17a_A22c_025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5vchb-q8t71
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-08-38_slicer7730.jpg: T6SS in Myxococcus Xanthus; 23nm
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-38/rawdata/</p>20140308_YWC093_10024.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        20140308_YWC093_10024_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
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