cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21794405
title: Fig2E_NB_luciferase_R2_LG378.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without a 99bp sequence from the STOML2 gene inserted. RNA was extracted and examined by northern blotting. Ethidium bromide-stained gel images are also included to show rRNA.
DOI: 10.6084/m9.figshare.21794114
title: Fig2G_RACE_STOML2_R3_LG366_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794309
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R2_LG297.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794309.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R2_LG297.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794312
title: Fig2G_RACE_STOML2_R2_LG364.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794312.v1
title: Fig2G_RACE_STOML2_R2_LG364.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794405.v1
title: Fig2E_NB_luciferase_R2_LG378.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without a 99bp sequence from the STOML2 gene inserted. RNA was extracted and examined by northern blotting. Ethidium bromide-stained gel images are also included to show rRNA.
DOI: 10.6084/m9.figshare.21794114.v1
title: Fig2G_RACE_STOML2_R3_LG366_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794156
title: Fig2G_RACE_STOML2_R1_LG361.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794156.v1
title: Fig2G_RACE_STOML2_R1_LG361.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794216
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R3_LG300.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794216.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R3_LG300.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794315
title: Fig2G_RACE_YKT6_R2_LG364.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794315.v1
title: Fig2G_RACE_YKT6_R2_LG364.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794234
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R2_LG297_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794234.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R2_LG297_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794288.v1
title: Fig2G_RACE_STOML2_R1_LG361.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794291
title: Fig2G_RACE_YKT6_R1_LG361.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21793604
title: Fig2G_RACE_YKT6_R2_LG364_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21793604.v1
title: Fig2G_RACE_YKT6_R2_LG364_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21793808
title: Fig2D_RACE_STOML2_YKT6_ExtFig3E_TUBA1B_R3_LG289.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X or vector and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6 and TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21793808.v1
title: Fig2D_RACE_STOML2_YKT6_ExtFig3E_TUBA1B_R3_LG289.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X or vector and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6 and TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21794288
title: Fig2G_RACE_STOML2_R1_LG361.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794291.v1
title: Fig2G_RACE_YKT6_R1_LG361.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794303
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R1_LG295_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794303.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R1_LG295_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793640
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R3_LG300.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793640.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R3_LG300.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793889
title: Fig2G_RACE_STOML2_R3_LG366.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21793889.v1
title: Fig2G_RACE_STOML2_R3_LG366.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21793694
title: Fig2D_RACE_STOML2_YKT6_R1_LG283.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X or vector and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6 and TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21793694.v1
title: Fig2D_RACE_STOML2_YKT6_R1_LG283.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X or vector and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6 and TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
DOI: 10.6084/m9.figshare.21793913
title: Fig2G_RACE_STOML2_R3_LG366.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21793913.v1
title: Fig2G_RACE_STOML2_R3_LG366.sgd

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.
DOI: 10.6084/m9.figshare.21794090
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R3_LG300_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21794090.v1
title: Fig2F_YKT6_ExtFig4C_TUBA1B_RACE_R3_LG300_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793952
title: Fig2F_STOML2_ExtFig4C_BCAP31_RACE_R2_LG297_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793952.v1
title: Fig2F_STOML2_ExtFig4C_BCAP31_RACE_R2_LG297_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) ("PR8") or A/Perth/16/2009 (H3N2) ("Perth") strains or vector, and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6, BCAP31 or TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. (Cal09 construct had an issue and these samples were re-done in separate experiments).
DOI: 10.6084/m9.figshare.21793994
title: Fig2G_RACE_STOML2_R1_LG361_annotated.tif

description: Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.