DOI: 10.5061/dryad.m2579m0
title: Data from: Multi-trophic β-diversity mediates the effect of environmental gradients on the turnover of multiple ecosystem functions

description: 1. Much effort has been devoted to better understanding the effects of
 environment and biodiversity on ecosystem functioning. However, few
 studies have moved beyond measuring biodiversity as species richness of a
 single group and/or focusing on a single ecosystem function. While there
 is a growing recognition that along environmental gradients, the
 compositional turnover of multiple trophic groups influences not only
 productivity but multiple ecosystem functions, we do not know yet which
 components of multi-trophic β-diversity influence which ecosystem
 functions. 2. Here, we captured the biodiversity found in soils using
 environmental DNA to study total soil multi-trophic β-diversity (between
 all taxa regardless of their trophic group association), horizontal
 β-diversities (β-diversities within trophic groups) and vertical
 β-diversity (β-diversity across trophic groups) along a 1000-m elevational
 gradient in the French Alps. Using path analyses, we quantified how these
 β-diversity components mediate the effects of environmental turnover on
 the turnover of multiple ecosystem functions (i.e. productivity,
 N-cycling, N-leaching) and overall multifunctionality. 3. While we found a
 strong direct effect of soil properties on the turnover of multiple
 ecosystem functions, we also found an indirect effect of climate and soil
 properties through multi-trophic β-diversity. More specifically, only
 total multi-trophic β-diversity and the horizontal β-diversity of
 saprophytic fungi were strongly related to the turnover of
 multifunctionality and, to a lower extent, the turnover of productivity
 and N-cycling. Our results suggest that decomposition processes and
 resulting nutrient availability are key to understand how ecosystem
 functions change along soil properties and climatic gradients in alpine
 ecosystems. 4. Beyond alpine systems, our study stresses the paramount
 importance of considering the mediating role of multi-trophic diversity in
 environmental change impacts on different ecosystem functions and their
 multifunctionality.
description: Soil properties
 variablesThis files contains
 following informations: "pH",
 "apparent_density_g.cm3", "%_total_porosity",
 "%_N_total", "%_organic_matter", "%_slope"
 Further description of the sampling and variables is descripted in the
 Mat&Met section of the papersoil_properties_variables.RDataClimatic VariablesThis files contains following informations: "gravel.g", "%_WFPS", "soil_temperature_celciusdeg", "Nb_FrostDays", "mean_Growth_Season_Length", "Topographical_Wetness_Index", "Topographic_Position_Index", "altitude_m", "mean.solar.gain.W.m2.10e5", "variance_Growth_Season_Length". Further description of the sampling and variables is descripted in the Mat&Met section of the paperclimatic_variables.RDataOTUs from several metabarcoding markorsThis data file contains raw OTUs from several metabarcoding markors (18S = all Eukaryots, AR = archea, BA = bacteria, FU = Fungi, GH = plants) For each marker, two files are provided, the "motu" one corresponds to taxonomic assignations of each MOTU, the "mxp" one is the motu*plot matrix. These informations allow to reproduce the trophic groups and the code is provided belowMETABAR_TOT.RDataEcosystem functionsThis files contains following informations: "ratio_C_N","leaching_NO3_µg_g","leaching_NH4_µg_g","N_NH4_produit_µgN_g_sol_j", #"ratio_N_DON_N_NO3&N_NH4","ratio_N_TDN_N_total","total_green_biomass_g_m2","NDVI_mean","NDVI_variance" Further description of the sampling and variables is descripted in the Mat&Met section of the paperecosystem_functions.RDataMain script to process, format and analyse environmental, multi-trophic and ecosystem functions dataThis script will allow the user to reproduce the main figures and analyses of "Multi-trophic β-diversity mediates the effect of environmental gradients on the turnover of multiple ecosystem functions". In particular, it built environmental and ecosystem functioning turnover matrices, built trophic groups and computes multiple assets of multi-trophic ß-diversity and computes multiple path analyses using lavaan. The associated script of functions is necessary to run this script.SCRIPT_Multi-trophic_β-diversity_mediates_the_effect_of_environmental_gradients_on_the_turnover_of_multiple_ecosystem_functions.RFunctions of the main scriptThis script holds the necessary functions to run the main script and reproduce data formatting & path analyses of "Multi-trophic β-diversity mediates the effect of environmental gradients on the turnover of multiple ecosystem functions"FONCTIONS_SCRIPT_Multi-trophic_β-diversity_mediates_the_effect_of_environmental_gradients_on _the_turnover_of_multiple_ecosystem_functions.R