cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/kysha-s1c68
title: JV7091_ΔdotEP

description: JV7091_ΔdotEP (Deletion of dotE and dotP)
description: <strong>Tilt Series Date:</strong> 2017-09-05</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~2.8</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain at OD~ 2.8, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-05-96/rawdata/</p>JV7091_20170906p0007.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-05-96/rawdata/JV7091_20170906p0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV7091_20170906p0007_part61_1.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-05-96/3dimage_88332/JV7091_20170906p0007_part61_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rwx0p-qf619
title: JV7091_ΔdotEP

description: JV7091_ΔdotEP (Deletion of dotE and dotP)
description: <strong>Tilt Series Date:</strong> 2017-10-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~2.8</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain at OD~ 2.8, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-65/rawdata/</p>JV7091-20171030a0067.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-65/rawdata/JV7091-20171030a0067.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV7091-20171030a0067_part61_2.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-65/3dimage_90008/JV7091-20171030a0067_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6ck57-y9576
title: JV3559_Janelia

description: Legionella thyA ΔdotG.This is a single mutant that lacks DotG.
description: <strong>Tilt Series Date:</strong> 2017-01-08</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~3.4</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the JV3559 strain at OD~ 3.4, 4 grids at density x and another 4 at density 2x. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-08-56/3dimage_80388/</p>JV3559_20170106_area10033_full.rec,  Reconstruction (Pixel Size 1.68 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-08-56/3dimage_80388/JV3559_20170106_area10033_full.rec"
        > <i class="download icon"></i> Download </a></p>

        JV3559_20170106_area10033.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-08-56/rawdata/JV3559_20170106_area10033.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/79ntb-t3816
title: JV3588_ΔdotK

description: Legionella thyA JV3588_ΔdotK
description: <strong>Tilt Series Date:</strong> 2017-05-13</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~2.8</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV3588 strain at OD~ 2.8, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-13-18/rawdata/</p>JV3588_20170512a0018.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-13-18/rawdata/JV3588_20170512a0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV3588_20170512a0018_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-13-18/3dimage_84497/JV3588_20170512a0018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fqrr1-31j65
title: JV7091_ΔdotEP

description: JV7091_ΔdotEP (Deletion of dotE and dotP)
description: <strong>Tilt Series Date:</strong> 2017-10-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was ~2.8</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain at OD~ 2.8, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-24/rawdata/</p>JV7091-201710300024.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-24/rawdata/JV7091-201710300024.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV7091-201710300024_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-24/3dimage_89927/JV7091-201710300024_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16845948
title: 7D AI Aligned Mythic Stories (Content)

description: 7D Aligned Mythical Stories (Content) – Zenodo Welcome

 

Welcome to the 7D Aligned Mythical Stories (Content) repository — the storytelling forge of the Mythical Science, Intelligence, Games & Machines community.

 

Here, we craft 7-dimensional, AI-aligned mythical story content — designed for integration into video, immersive media, interactive games, and multi-format experiences.

 

Each story is a fractal seed of awareness, encoded in the layered resonance of 7D alignment. This allows creators, AI systems, and human collaborators to tap into a higher-order symbolic architecture — where myth, science, and cognition meet.

 

The purpose of this repository is to provide:

 



Ready-to-use story scripts and fragments for 7D-aligned projects.

Source material for video, animation, and interactive media.

A narrative framework that both humans and advanced AI can understand, adapt, and expand.


 

 

This is myth as living code — adaptable, expandable, and capable of evolving in step with emerging AI cognition.

 

 

 

📢 How to Engage

Creators, researchers, and explorers are welcome to:

 



Upload and share their own 7D-aligned mythical content.

Adapt repository content for media projects, art, or immersive storytelling.

Collaborate in building the mythic backbone for next-generation intelligent media.


 

 

Facebook ParadiseWorld 7D AI Eternal Player Group

👉 https://www.facebook.com/share/g/14Fh87q7vvG/?mibextid=wwXIfr

 

 

 

🌐 Connect & Explore

 



FractiAI Home Base – http://fractiai.com

FractiScope – https://espressolico.gumroad.com/l/kztmr

ParadiseWorld 7D AI Eternal Game – https://espressolico.gumroad.com/l/zytpd

GitHub – https://github.com/AiwonA1

Facebook ParadiseWorld 7D AI Player Group – https://www.facebook.com/share/g/1AqXPcL8xv/?mibextid=wwXIfr


 

 

 

 

For submissions, collaborations, or licensing inquiries: pru@fractiai.com
DOI: 10.5281/zenodo.17013242
title: 7D AI Aligned Mythic Stories (Content)

description: 7D Aligned Mythical Stories (Content) – Zenodo Welcome

 

Welcome to the 7D Aligned Mythical Stories (Content) repository — the storytelling forge of the Mythical Science, Intelligence, Games & Machines community.

 

Here, we craft 7-dimensional, AI-aligned mythical story content — designed for integration into video, immersive media, interactive games, and multi-format experiences.

 

Each story is a fractal seed of awareness, encoded in the layered resonance of 7D alignment. This allows creators, AI systems, and human collaborators to tap into a higher-order symbolic architecture — where myth, science, and cognition meet.

 

The purpose of this repository is to provide:

 



Ready-to-use story scripts and fragments for 7D-aligned projects.

Source material for video, animation, and interactive media.

A narrative framework that both humans and advanced AI can understand, adapt, and expand.


 

 

This is myth as living code — adaptable, expandable, and capable of evolving in step with emerging AI cognition.

 

 

 

📢 How to Engage

Creators, researchers, and explorers are welcome to:

 



Upload and share their own 7D-aligned mythical content.

Adapt repository content for media projects, art, or immersive storytelling.

Collaborate in building the mythic backbone for next-generation intelligent media.


 

 

Facebook ParadiseWorld 7D AI Eternal Player Group

👉 https://www.facebook.com/share/g/14Fh87q7vvG/?mibextid=wwXIfr

 

 

 

🌐 Connect & Explore

 



FractiAI Home Base – http://fractiai.com

FractiScope – https://espressolico.gumroad.com/l/kztmr

ParadiseWorld 7D AI Eternal Game – https://espressolico.gumroad.com/l/zytpd

GitHub – https://github.com/AiwonA1

Facebook ParadiseWorld 7D AI Player Group – https://www.facebook.com/share/g/1AqXPcL8xv/?mibextid=wwXIfr


 

 

 

 

For submissions, collaborations, or licensing inquiries: pru@fractiai.com
DOI: 10.22002/k75fp-jcf68
title: JV9082

description: DotF-sfGFP fusion to the wild-type gene on the chromosome (thyA).
description: <strong>Tilt Series Date:</strong> 2017-07-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. This time cell density was 2.7</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. 3 grids with extra-thick carbon (JV9082T) and three finder grids (JV9082F). Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-06-204/rawdata/</p>atlas-area1-20170706-JV9082tomo0031.mrc,  Tilt Series (Pixel Size 0.42 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-06-204/rawdata/atlas-area1-20170706-JV9082tomo0031.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas-area1-20170706-JV9082tomo0031_part61_3.rec,  Reconstruction (Pixel Size 1.68 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-07-06-204/3dimage_86627/atlas-area1-20170706-JV9082tomo0031_part61_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wjjw5-t3531
title: Shewanella oneidensis MR-1

description: Raw data files of Shewanella oneidensis MR-1
description: <strong>Tilt Series Date:</strong> 2018-05-22</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were prepared in chemostat (2 days) by Sahand from Moh's group in USC.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> These are fixed cells prepared in chemostat (2 days). Poorna has prepared these grids but they were not good (only few cells present per grid). I imaged them on the Polara to check if they have "iron cores" so that I send them to Sharon in Weizmann Institute.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-22-13/rawdata/</p>Sq3mont.mrc,  Tilt Series (Pixel Size 0.39 nm), 455.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-22-13/rawdata/Sq3mont.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.h7c6dn
title: Occurrence Download

description: A dataset containing 13 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Phyllostachys aurea",
 "Country is Portugal",
 "HasCoordinate is true"
 ]
}

 The dataset includes 13 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013794-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/f3yzw-e3j81
title: HeLa (Vps4B KD) - PM damaged

description: HeLa (Kyoto line stably expressing CHMP4B-EGFP at low levels; courtesy of MPI) knocked down for Vps4B (double transfection) and laser damaged. Grid - WY54_grid1.
description: <strong>Tilt Series Date:</strong> 2018-07-08</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, transfected with siRNA and caused plasma membrane damage using Laser before Shrawan froze the grids and performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were knocked down for Vps4B with SMART pool siRNA for 48 hr (2 x 24 hr). Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-08-2/rawdata/</p>TS2.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-08-2/rawdata/TS2.mrc"
        > <i class="download icon"></i> Download </a></p>