cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.7bj715s
title: Data from: Architectural constraints, male fertility variation and biased floral morph ratios in tristylous populations

description: Tristyly is a genetic polymorphism in which populations are comprised of
 three floral morphs (mating types) differing reciprocally in sex-organ
 height. Intermorph (disassortative) mating governed by a trimorphic
 incompatibility system should result in 1:1:1 morph ratio at equilibrium,
 but both deterministic and stochastic processes can cause skewed morph
 ratios in tristylous populations. Here, we investigate mechanisms causing
 morph-ratio bias in Pontederia parviflora, an emergent aquatic native to
 tropical America. We compared reproductive traits among morphs and
 surveyed 71 populations to determine patterns of morph-ratio bias. We then
 used simulation models of morph-frequency dynamics to test the hypothesis
 that morph-specific differences in pollen production and their influence
 on male fertility can explain patterns of morph-ratio bias. Ninety-seven
 percent of populations that we sampled were tristylous, but with a
 significant excess of the short-styled morph and a deficiency of the
 long-styled morph. Atypically for a tristylous species, mid-level anthers
 of the short-styled morph produced over twice as much pollen compared with
 the corresponding anthers of the long-styled morph. Our computer models
 incorporating this difference in male fertility resulted in morph ratios
 not significantly different from the average frequencies from our survey
 suggesting that the short-styled morph is more successful than the
 long-styled morphs in siring ovules of the mid-styled morph. We propose
 that the difference in male fertility between morphs may be a non-adaptive
 consequence of a developmental constraint caused by the architecture of
 tristyly in Pontederiaceae.
description: Floral morphs and flower
 measurements of P. parviflora in the Pantanal Wetlandssheet1: Geographic
 coordinates, floral-morph frequencies, inflorescence counts,
 population-size estimates for sampled populations of Pontederia parviflora
 in the Pantanal Wetlands. sheet2: Measurements of flower length, petal
 width, perianth-tube aperture and base of corolla width, and sex organs
 (l, m and s denotes anthers levels) for the three floral morphs of
 Pontederia parviflora in five populations sampled in the Pantanal
 wetlands; sheet3: Number of senesced flowers, open flowers, buds and total
 flower number of inflorescences of the three floral morphs of Pontederia
 parviflora; sheet4: number of aborted fruit and seed set in
 infructescences of the three floral morphs in five populations in the
 Pantanal; sheet5: Mean pollen size and pollen number of the l-, m- and
 s-anthers of the L-, M-, and S-morph of Pontederia parviflora sampled in
 five populations in the Pantanal.Data_P_parviflora.xlsx
DOI: 10.5061/dryad.9811959
title: Data from: DNA methylation predicts immune gene expression in introduced house sparrows (Passer domesticus)

description: Populations undergoing range expansions are often faced with novel
 selective pressures, and to cope with such challenges, populations must
 either adapt quickly or exhibit phenotypic plasticity. This latter option
 allows for rapid phenotypic adjustments and persistence in novel
 environments, and thus could be advantageous at range‐edges. Our previous
 research on house sparrows in Kenya—a site of ongoing range expansion— and
 a growing literature suggests that invasion success is facilitated by
 epigenetic regulation of gene expression. Previously, we found (i)
 differences in the expression of a microbial surveillance gene (i.e.
 Toll‐like receptor 4—TLR4), and (ii) extensive variation in genome‐wide
 DNA methylation among house sparrows across Kenya. Here, our goal was to
 investigate whether these two observations are related, specifically
 whether DNA methylation within a target sequence upstream of the TLR4
 transcription start site is associated with variation in TLR4 expression.
 We found that DNA methylation in the aforementioned region was quite
 variable among individuals, and variation at one CpG site predicted
 differences in TLR4 expression. Moreover, we found genetic variation
 within the same sequence upstream of the TLR4 exon, but this variation did
 not predict TLR4 expression. To our knowledge, this is the first study to
 demonstrate an association between DNA methylation and the expression of
 an ecologically relevant trait in a range‐expanding vertebrate.
description: Kilvitis et al. Dryad
 DataThe excel sheet contains
 identifier data, TLR4 expression, SNP and methylation
 data.Dryad
 Data.xlsx
DOI: 10.5061/dryad.c1t54v2
title: Data from: The evolution of gametic compatibility and compatibility groups in the sea urchin Mesocentrotus franciscanus: an avenue for speciation in the sea

description: The generation of reproductive incompatibility between groups requires a
 rare genotype with low compatibility to increase in frequency. We tested
 the hypothesis that sexual conflict driven by the risk of polyspermy can
 generate compatibility groups in gamete recognition proteins (GRPs) in the
 sea urchin Mesocentrotus franciscanus. We examined variation in the sperm
 (bindin) and egg (EBR1) GRPs, how this variation influences fertilization
 success and how allele frequencies shift in these GRPs over time. The EBR1
 gene is a large, 4595 amino acid, protein made up of 27 TSP and 20 CUB
 repeats. Two TSP and two CUB repeats each demonstrate two common
 non-synonymous haplotypes (alleles). Sperm bindin and one of these EBR1
 repeats (TSP8) shift allele frequencies from one common to two common
 types over an approximate 200 year interval associated with the removal of
 predatory sea otters and rising sea urchin abundances; the egg receptor
 shifts first, followed by the sperm ligand. Laboratory crosses indicate
 that the historically common sperm and egg gamete recognition proteins
 have high compatibility as do the new common proteins, with mismatches
 having lower compatibility. This process of creating compatibility groups
 sets the stage for reproductive isolation and speciation.
description: Gametc compatibity in
 Mesocentrotus franciscanusWorksheets (1) GRP
 genotypes as a function of body size, (2) fertilization success, (3-6)
 EBR1 sequence dataEvolution data files
 2019.xlsx
DOI: 10.17632/jkrfp9dn5g.2
title: Integrating GIS-Based Groundwater Potential Mapping and Geophysical Surveys in Fractured Aquifers: a case study in Brazil

description: This dataset was developed to support the study “Decision-Support Mapping for Sustainable Groundwater Use in Fractured Urban Aquifers: A GIS and Geophysical Approach in Brazil” and provides the spatial and geophysical information used to identify groundwater exploitation potential in a heterogeneous fractured aquifer system. The dataset compiles geological, geomorphological, hydrogeological, and geophysical attributes integrated within a Geographic Information System (GIS) framework to produce a groundwater potential map for an urban environment.

This dataset aims to ensure transparency, reproducibility, and reuse of the proposed methodology, enabling other researchers, water managers, and planners to apply, adapt, or compare the approach in similar geological and urban contexts. By reducing uncertainties in well siting and supporting sustainable groundwater management, the dataset contributes to broader efforts toward urban water security and the responsible use of fractured aquifer systems.
DOI: 10.5061/dryad.sf2gt4k
title: Data from: Survival is negatively related to basal metabolic rate in tropical Andean birds

description: 1. Life history theory postulates that physiological traits, such as
 energy metabolism, can be understood in terms of allocation trade-offs
 between self-maintenance and reproduction over an organism’s lifespan, and
 data show that metabolic intensity and survival vary inversely with
 latitude, with tropical birds exhibiting a ‘slow’ pace of life relative to
 temperature species. However, tropical regions harbor strong environmental
 gradients of their own, and it remains to be shown whether similar life
 history trade-offs between metabolism and longevity are reflected among
 tropical birds of the same latitude. 2. We estimated apparent annual
 survival in 37 species of tropical passerine birds along an elevational
 gradient (400–3000 m) in Peru to test whether variation in survival was
 influenced by basal metabolic rate (BMR; estimated at the same sites),
 elevation or both factors. We used path analysis to test our prediction
 that survival would decline as BMR increased, while accounting for the
 potential direct effects of elevation on survival due to differences in
 predation pressure or environmental conditions as well as potential
 indirect effects of elevation on BMR via temperature and the costs of
 thermoregulation. 3. Higher BMR in tropical passerine birds predicted
 lower apparent survival, regardless of the elevation at which species
 occurred. In addition, elevation had a direct negative effect on apparent
 survival, perhaps due to harsher abiotic conditions, low site fidelity, or
 both at high elevations. 4. We provide evidence of a link between
 metabolic rate and longevity previously undescribed in populations of
 free-living birds. Our results illustrate that tropical montane species
 may be characterized by a unique suite of traits in their pace of life, in
 which BMR does not differ from lowland birds, but survival does.
description: Life history
 measurements of tropical Andean birdsThese data were
 collected in the field from the Manu Biosphere Reserve, southeastern Peru
 between 2011-2016. Abbreviations of column headings are for apparent adult
 survival, phi; standard error, se; the top model selected using QAIC,
 QAIC_top_model; the mean elevation of capture locations for the species,
 cap_elev; basal metabolic rate, bmr; and estimated c-hat for each model
 used to adjust QAIC; chat.scholer_etal_FE2019_data.csv
DOI: 10.17632/k3kxy2sh3y
title: Microstructures and Properties of Nickel-Aluminum-Bronze Coating by Laser Wire Additive Manufacturing

description: This study investigates the microstructure, mechanical properties, and corrosion resistance of nickel-aluminum bronze (NAB) coatings fabricated on Q235 steel using laser fused wire additive manufacturing (LFWAM). The coatings were characterized using X-ray diffraction (XRD), electron backscatter diffraction (EBSD), and electrochemical analysis. The main phases identified in the NAB coating are α-phase Cu, κⅠ-phase AlFe₃, and κⅡ-phase AlNi. The microstructure is fine, dense, and uniform, with an average microhardness of 259 HV0.2. The average coefficient of friction and wear rate are 0.317 and 1.694 × 10-6g/(N·m), respectively. Corrosion tests show a corrosion potential of -0.21 V and a corrosion current density of 6.201 × 10-7A/cm². The solid solution of Al and Ni in the coating enhances strength through solid solution strengthening. Grain refinement from Al and the formation of a dense oxide layer, along with the presence of the Ni-rich κⅡ-phase, significantly improve the coating’s strength, wear resistance, and corrosion resistance, contributing to its excellent overall performance.
DOI: 10.5061/dryad.1qc2r30
title: Data from: Inhibition of Smurf2 translation by miR-322/503 protects from ischemia reperfusion injury by modulating EZH2/Akt/GSK3β signaling

description: BACKGROUND: myocardial ischemia/reperfusion (I/R) is a common and lethal
 disease that threatens people's life worldwide. The underlying
 mechanisms are under intensive study and yet remain unclear. Here, we
 explored the function of miR-322/503 in myocardial I/R injury. METHODS: We
 used isolated rat perfused heart as an in vivo model and H9c2 cells
 subjected with the oxygen and glucose deprivation followed by reperfusion
 (OGD/R) as in vitro model to study myocardial I/R injury. TTC staining was
 used to measure the infarct size and TUNEL staining was used to examine
 apoptosis. qRT-PCR and western blot were used to determine expression
 levels of miR-322/503, Smurf2, EZH2, p-Akt, p-GSK3β. RESULTS:
 Overexpression of miR-322/503 decreased infarct size, inhibited cell
 apoptosis and promoted cell proliferation through up-regulation of p-Akt
 and p-GSK3β. Thus, the expression of miR-322/503 was reduced during I/R
 process. On the molecular level, miR-322/503 directly bound Smurf2 mRNA
 and suppressed its translation. Smurf2 ubiquitinated EZH2 and degraded
 EZH2 which could activate Akt/GSK3β signaling. CONCLUSIONS: Our study
 demonstrates that miR-322/503 plays a beneficial role in myocardial I/R
 injury. By inhibition of Smurf2 translation, miR-322/503 induces EZH2
 expression and activates Akt/GSK3β pathway, thereby protecting cells from
 ischemia reperfusion injury.
description: Figure Supple1. (A)
 qRT-PCR analysis of miR-322, miR-503 mRNA levels in in vivo myocardial I/S
 model. (B). Cell apoptosis was determined by Flow analysis in H9c2 cells
 transfected with shNC or shSmurf2. (C). Cell apoptosis was determined by
 Flow analysis in H9c2 cells with EZH2 overexpression. (D). Cell apoptosis
 was determined by Flow analysis in H9c2 cells with Smurf2 overexpression.
 All the results were shown as mean ± SD (n = 3); * p&lt; 0.05 and ** p
 &lt; 0.01.Supplement.tif
DOI: 10.17632/k3kxy2sh3y.2
title: Microstructures and Properties of Nickel-Aluminum-Bronze Coating by Laser Wire Additive Manufacturing

description: This study investigates the microstructure, mechanical properties, and corrosion resistance of nickel-aluminum bronze (NAB) coatings fabricated on Q235 steel using laser fused wire additive manufacturing (LFWAM). The coatings were characterized using X-ray diffraction (XRD), electron backscatter diffraction (EBSD), and electrochemical analysis. The main phases identified in the NAB coating are α-phase Cu, κⅠ-phase AlFe₃, and κⅡ-phase AlNi. The microstructure is fine, dense, and uniform, with an average microhardness of 259 HV0.2. The average coefficient of friction and wear rate are 0.317 and 1.694 × 10-6g/(N·m), respectively. Corrosion tests show a corrosion potential of -0.21 V and a corrosion current density of 6.201 × 10-7A/cm². The solid solution of Al and Ni in the coating enhances strength through solid solution strengthening. Grain refinement from Al and the formation of a dense oxide layer, along with the presence of the Ni-rich κⅡ-phase, significantly improve the coating’s strength, wear resistance, and corrosion resistance, contributing to its excellent overall performance.
DOI: 10.5061/dryad.8823vb0
title: Data from: Detection of environmental and morphological adaptation despite high landscape genetic connectivity in a pest grasshopper (Phaulacridium vittatum)

description: Widespread species that exhibit both high gene flow and the capacity to
 occupy heterogeneous environments make excellent models for examining
 local selection processes along environmental gradients. Here we evaluate
 the influence of temperature and landscape variables on genetic
 connectivity and signatures of local adaptation in Phaulacridium vittatum,
 a widespread agricultural pest grasshopper, endemic to Australia. With
 sampling across a 900 km latitudinal gradient, we genotyped 185 P.
 vittatum from 19 sites at 11,408 single nucleotide polymorphisms (SNPs)
 using ddRAD sequencing. Despite high gene flow across sites (pairwise Fst
 = 0.0003-0.08), landscape genetic resistance modelling identified a
 positive non-linear effect of mean annual temperature on genetic
 connectivity. Urban areas and water bodies had a greater influence on
 genetic distance among sites than pasture, agricultural areas, and forest.
 Together, Fst outlier tests and Environmental Association Analysis (EAA)
 detected 242 unique SNPs under putative selection, with the highest
 numbers associated with latitude, mean annual temperature, and body size.
 A combination of landscape genetic connectivity analysis together with EAA
 identified mean annual temperature as a key driver of both neutral gene
 flow and environmental selection processes. Gene annotation of putatively
 adaptive SNPs matched with gene functions for olfaction, metabolic
 detoxification and ultra-violet light shielding. Our results imply that
 this widespread agricultural pest has the potential to spread and adapt
 under shifting temperature regimes and land cover change.
description: 3257_snps_PvittFiltered dataset PED format consisting of 3,257 SNPs genotyped in 185 individuals of Phaulacridium vittatum.3257_snps_PvittDatafile (map format) consisting names of 3257 loci11408_snps_PvittFiltered dataset consisting of 11,408 SNPs genotyped in 185 individuals used for adaptive genetic analysis11408_snps_PvittMap file containing 11,408 SNP loci name.Model_selection_LandcoverR script to perform model selectionalpha_gamma_valuesDatafile consisting values of of alpha and gamma. Required to generate resistance surfaces.Landcover_150m_resRe-classified landcover raster file used to generate multiple resistance surfaces.BIO1_Mean_annual_tempAnnual mean temperature raster file used to generate multiple resistance surfacesMorphology_185_ind_PvittMorphological data of 185 individuals used to test morph-environment association in LFMM.
DOI: 10.17632/v8zgyg4pmj
title: Journal of Hazardous Materials

description: Data of “Long-term effect of sulfhydryl modified attapulgite on stabilizing Zn and Mn from Pb-Zn tailings under different hydrological conditions”
DOI: 10.17632/v8zgyg4pmj.1
title: Journal of Hazardous Materials

description: Data of “Long-term effect of sulfhydryl modified attapulgite on stabilizing Zn and Mn from Pb-Zn tailings under different hydrological conditions”
DOI: 10.5061/dryad.0898ss6
title: Data from: Environmental heterogeneity decreases reproductive success via effects on foraging behaviour

description: Environmental heterogeneity shapes the uneven distribution of resources
 available to foragers, and is ubiquitous in nature. Optimal foraging
 theory predicts that an animal’s ability to exploit resource patches is
 key to foraging success. However, the potential fitness costs and benefits
 of foraging in a heterogeneous environment are difficult to measure
 empirically. Heterogeneity may provide higher quality foraging
 opportunities, or alternatively could increase the cost of resource
 acquisition because of reduced patch density or increased competition.
 Here, we study the influence of physical environmental heterogeneity on
 behaviour and reproductive success of black-legged kittiwakes, Rissa
 tridactyla. From GPS tracking data at 15 colonies throughout their British
 and Irish breeding range, we found that environments that were physically
 more heterogeneous were associated with longer trip duration, more time
 spent actively foraging whilst away from the colony, increased overlap of
 foraging areas between individuals, and lower breeding success. These
 results suggest that in more heterogeneous environments, there is greater
 competition between individuals for finite resources, which comes at a
 cost to reproduction. Resource hotspots are often considered to be
 beneficial, as individuals can learn to exploit them if sufficiently
 predictable. However, we demonstrate here that such fitness gains can be
 countered by greater competition in more heterogeneous environments.
description: Code_kittiwake
 heterogeneityR Code to calculate
 environmental heterogeneity and the links with foraging
 dynamicsEnvironmentEnvironmental variables at random locations around kittiwake colonies used to calculate environmental heterogeneityKittiwake trip metrics and successTrip metrics, reproductive success and heterogeneity used in paper analysesKittiwake.csvHMMbehavioursProportion of time spent in forage/transit/restFoverlapOverlap between individual foraging areasFsizeSize of individual foraging areas
DOI: 10.17632/tw3rx4wcnn
title: Osteopontin-4 (OPN-4) suppresses tumor progression features whilst sensitizing c643 anaplastic thyroid cells to sorafenib

description: This processed dataset is associated with the study “Osteopontin-4 (OPN-4) suppresses tumor progression features whilst sensitizing c643 anaplastic thyroid cells to sorafenib.” The dataset includes results from cell proliferation, viability and migration assays, cell death and cell cycle analyses, as well as experiments performed in 3D culture conditions combined with a microfluidic device. Additionally, the dataset contains data regarding the cellular response to sorafenib treatment in the context of OPN-4 expression modulation in the c643 cell line.
All experiments were conducted in duplicate or triplicate, with at least three independent biological replicates. The Excel file is organized into sheets identified according to the corresponding figures presented in the article, providing essential experimental information to support data interpretation and reuse.
DOI: 10.17632/tw3rx4wcnn.1
title: Osteopontin-4 (OPN-4) suppresses tumor progression features whilst sensitizing c643 anaplastic thyroid cells to sorafenib

description: This processed dataset is associated with the study “Osteopontin-4 (OPN-4) suppresses tumor progression features whilst sensitizing c643 anaplastic thyroid cells to sorafenib.” The dataset includes results from cell proliferation, viability and migration assays, cell death and cell cycle analyses, as well as experiments performed in 3D culture conditions combined with a microfluidic device. Additionally, the dataset contains data regarding the cellular response to sorafenib treatment in the context of OPN-4 expression modulation in the c643 cell line.
All experiments were conducted in duplicate or triplicate, with at least three independent biological replicates. The Excel file is organized into sheets identified according to the corresponding figures presented in the article, providing essential experimental information to support data interpretation and reuse.
DOI: 10.5061/dryad.kj67m68
title: Data from: Meta-analysis reveals enhanced growth of marine harmful algae from temperate regions with warming and elevated CO2 levels

description: Elevated pCO2 and warming may promote algal growth and toxin production,
 and thereby possibly support the proliferation and toxicity of HABs. Here,
 we tested whether empirical data supports this hypothesis using a
 meta-analytic approach and investigated the responses of growth rate and
 toxin content or toxicity of numerous marine and estuarine HAB species to
 elevated pCO2 and warming. Most of the available data on HAB responses
 towards the two tested climate change variables concerns dinoflagellates,
 as many members of this phytoplankton group are known to cause HAB
 outbreaks. Toxin content and toxicity did not reveal a consistent response
 towards both tested climate change variables, while growth rate increased
 consistently with elevated pCO2 . Warming also led to higher growth rates,
 but only for species isolated at higher latitudes. The observed gradient
 in temperature growth responses shows the potential for enhanced
 development of HABs at higher latitudes. Increases in growth rates with
 more CO2 may present an additional competitive advantage for HAB species,
 particularly as CO2 was not shown to enhance growth rate of other non-HAB
 phytoplankton species. However, this may also be related to the difference
 in representation of dinoflagellate and diatom species in the respective
 HAB and non-HAB phytoplankton groups. Since the proliferation of HAB
 species may strongly depend on their growth rates, our results warn for a
 greater potential of dinoflagellate HAB development in future coastal
 waters, particularly in temperate regions.
description: Harmful algal growth
 rates and toxin contents under climate changeGrowth rates and toxin
 content of multiple harmful algal species and strains derived from single
 strain laboratory culture experiments performed under varying temperature
 and/or CO2 conditions.Data.csv