DOI: 10.5061/dryad.pvmcvdnwv
title: An amphipol-stabilized multi-pass transmembrane protein as an immunogen to generate mouse memory B cells against native VMAT2

description: Complex integral membrane proteins with the property of multiple
 transmembrane-spanning regions are large protein families, including
 G-protein-coupled receptors (GPCR), ion channels, and transporters. Due to
 their essential roles in sensing and processing signals, they are the
 primary drug targets of more than half of the approved drugs—majorly small
 molecules. Although antibodies succeeded in the pharmaceutical markets,
 they are rare in modulating complex integral membrane proteins with
 favorable properties. Two significant limitations in such antibody
 discovery are the preparation of correctly folded antigens and the
 generation of antibodies against the natural conformation. Here, we
 developed an amphipol-trapped antigen as an immunogen and induced
 efficient mouse memory B cell responses. We generated antibodies
 unbiasedly by culturing single memory B cells and characterized their
 specificities. We implemented our strategy to generate high-affinity
 antibodies against the native conformation of vesicular monoamine
 transporter 2 (VMAT2), demonstrating the potential use in discovering
 antibodies against membrane proteins, including multi-pass transmembrane
 protein, for therapeutics.
description:   Eighty-eight antibody sequences (heavy chain
 and light chains) discovered in this study using Sanger sequencing are
 included in this dataset. They were isolated from mouse memory B
 cells.    
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
    
description: # An amphipol-stabilized multi-pass transmembrane protein as an immunogen
 to generate mouse memory B cells against native VMAT2
 [https://doi.org/10.5061/dryad.pvmcvdnwv](https://doi.org/10.5061/dryad.pvmcvdnwv) ## Antibody\_heavy\_and\_light\_chain.rtf 88 antibody heavy and light chains discovered in this study were included in the dataset. They were from mouse memory B cells. This file is in FASTA format.