DOI: 10.5061/dryad.dbrv15fcp
title: Plant-microbe interactions derive the rhizosphere microbial assembly and nitrogen cycling in a subtropical forest

description: 1. Interactions between plants and soil microorganisms in the rhizosphere
 are vital for maintaining the nutrient cycle and stability of terrestrial
 ecosystems. Nitrogen, closely related to carbon (C) cycling and ecosystem
 productivity, undergoes transformation by soil microorganisms into forms
 that can be assimilated by plants. However, the mechanism by which plants
 impact nitrogen (N) cycling in acid subtropical forest soil by governing
 the rhizosphere microbial community remains unclear. 2. This study
 examined the impact of host phylogeny, root traits, and soil properties on
 microbial communities and N cycles in the rhizospheres of Fagaceae and
 Lauraceae, the two dominant families in the subtropical forest of
 Heishiding Natural Reserve in southern China. 3. Our findings revealed
 that host phylogeny dominates the variation in the richness and
 composition of the rhizosphere microbial community. Furthermore,
 antibacterial exudates from Lauraceae might reduce the stability of
 microbial network structure and enrich fatty acid biosynthesis function
 for antimicrobial resistance. Net nitrification rates (NNR) and nitrifying
 enzyme activities (NEA) of arbuscular mycorrhizae (AM) trees of Lauraceae
 were significantly higher than that of ectomycorrhizae (ECM) trees of
 Fagaceae. The positive relationships among NNR, NEA, and root nitrogen
 content (RNC) suggests a close correlation between soil nitrification
 activity and N uptake efficiency by plants. Complete ammonia oxidizer
 (comammox) dominated the ammonia-oxidizing communities of these acid
 soils, followed by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing
 bacteria (AOB). The niche differentiation among these groups was strongly
 influenced by soil properties and plant traits, which in turn affects
 plant N uptake efficiency. 4. In conclusion, we conducted a comprehensive
 analysis of the root-mediated plant-microbe interactions and the regulated
 microbial N cycles in the rhizosphere soils of an acid subtropical forest.
 These findings are crucial for elucidating the biological mechanisms
 guiding microbial assembly in the rhizosphere and refining biological
 strategies to mitigate N loss in forest ecosystems. --
description: # Plant-microbe interactions derive the rhizosphere microbial assembly and
 nitrogen cycling in a subtropical forest
 [https://doi.org/10.5061/dryad.dbrv15fcp](https://doi.org/10.5061/dryad.dbrv15fcp) ## Description of the data and file structure Rhizosphere soil samples of Lauraceae and Fagaceae were collected from typical subtropical forests in Heishingting Nature Reserve, South China. DNA of soil samples was extracted and 16S rRNA gene amplicon sequencing was performed. ### Files and variables #### File: 16Sv4.11.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.12.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.13.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.14.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.15.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.16.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.17.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.18.fastq **Description:** Castanopsis fordii,Fagaceae | | | :- | #### File: 16Sv4.181.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.182.fastq **Description:**  Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.183.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.187.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.189.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.190.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.186.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.325.fastq **Description:** Lindera chunii,Lauraceae #### File: 16Sv4.327.fastq **Description:** Lindera chunii,Lauraceae #### File: 16Sv4.329.fastq **Description:** Lindera chunii,Lauraceae #### File: 16Sv4.324.fastq **Description:** Lindera chunii,Lauraceae #### File: 16Sv4.371.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 16Sv4.375.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 16Sv4.376.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 16Sv4.377.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 16Sv4.378.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 16Sv4.374.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 16Sv4.434.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 16Sv4.437.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 16Sv4.438.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 16Sv4.439.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 16Sv4.592.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 16Sv4.436.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 16Sv4.593.1.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 16Sv4.595.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 16Sv4.598.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 188.fastq **Description:** Cyclobalanopsis chungii,Fagaceae #### File: 16Sv4.594.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 328.fastq **Description:** Lindera chunii,Lauraceae #### File: 323.fastq **Description:** Lindera chunii,Lauraceae #### File: 326.fastq **Description:** Lindera chunii,Lauraceae #### File: 330.fastq **Description:** Lindera chunii,Lauraceae #### File: 440.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 373.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 379.fastq **Description:** Cryptocarya chinensis,Lauraceae #### File: 432.fastq **Description:** Lithocarpus litseifolius,Fagaceae #### File: 596.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 591.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: 600.fastq **Description:** Cinnamomum appelianum,Lauraceae #### File: b435.join.fastq **Description:** Lithocarpus litseifolius,Fagaceae ## Access information Other publicly accessible locations of the data: * NCBI Data was derived from the following sources: * [PRJNA1108040](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1108040)