DOI: 10.5061/dryad.p725k
title: Data from: Electromyographic permutation entropy quantifies diaphragmatic denervation and reinnervation

description: Spontaneous reinnervation after diaphragmatic paralysis due to trauma,
 surgery, tumors and spinal cord injuries is frequently observed. A
 possible explanation could be collateral reinnervation, since the
 diaphragm is commonly double-innervated by the (accessory) phrenic nerve.
 Permutation entropy (PeEn), a complexity measure for time series, may
 reflect a functional state of neuromuscular transmission by quantifying
 the complexity of interactions across neural and muscular networks. In an
 established rat model, electromyographic signals of the diaphragm after
 phrenicotomy were analyzed using PeEn quantifying denervation and
 reinnervation. Thirty-three anesthetized rats were unilaterally
 phrenicotomized. After 1, 3, 9, 27 and 81 days, diaphragmatic
 electromyographic PeEn was analyzed in vivo from sternal, mid-costal and
 crural areas of both hemidiaphragms. After euthanasia of the animals, both
 hemidiaphragms were dissected for fiber type evaluation. The
 electromyographic incidence of an accessory phrenic nerve was 76%. At day
 1 after phrenicotomy, PeEn (normalized values) was significantly
 diminished in the sternal (median: 0.69; interquartile range: 0.66–0.75)
 and mid-costal area (0.68; 0.66–0.72) compared to the non-denervated side
 (0.84; 0.78–0.90) at threshold p&lt;0.05. In the crural area,
 innervated by the accessory phrenic nerve, PeEn remained unchanged (0.79;
 0.72–0.86). During reinnervation over 81 days, PeEn normalized in the
 mid-costal area (0.84; 0.77–0.86), whereas it remained reduced in the
 sternal area (0.77; 0.70–0.81). Fiber type grouping, a histological sign
 for reinnervation, was found in the mid-costal area in 20% after 27 days
 and in 80% after 81 days. Collateral reinnervation can restore diaphragm
 activity after phrenicotomy. Electromyographic PeEn represents a new,
 distinctive assessment characterizing intramuscular function following
 denervation and reinnervation.
description: PONE-D-14-21748R1Explanation: Electromyography (EMG) data is displayed in 5 folders, that are named „EMG_Day1”, “EMG_Day3”, “EMG_Day9”, “EMG_Day27”, “EMG_Day81” respectively. Each folder contains data from rats that were phrenicotomized at the respective timepoint. The rats are identified by the following numbers: Day 1: 1409, 1410, 1411,1412,1413,1414 Day3: 1815, 1816, 1817, 1818, 1819, 1820 Day 9: 1821, 1822, 1823, 1824, 1825, 1826 Day 27: 1720, 1721, 1722, 1723, 1725 Day 81: 1484, 1485, 1486, 1487, 1488, 1489, 1490, 1491, 1492, 1493 Folder "EMG" contains primary EMG data (e.g. bursts). According to the Materials and Methods Section in the Manuscript, a mean PeEn was created using the respective EMG bursts. The mean PeEn for each subject (sorted timepoint-wise) is displayed in the pdf-file "EMG_PeEN". Primary data is grouped timepoint-wise, including one folder for each subject. EMG bursts are provided as .wav-files. It has to be considered that the following terminology concerning the area of the diaphragm the EMG bursts (.wav-files) originated was used in the respective folders: "animal number_hinten" = "animal number_crural" "animal number_mitte" = "animal number_mid-costal" "animal number_vorne" = "animal number_sternal" "animal_number_kl" = "animal number_non-denervated side"