cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.413
title: Soil microarthropods in reforested area of dipterocarpus seedlings at different stages

description: The diversity and abundance of soil microarthropods and physical and biological factors had been evaluated in 1-year and 2-year reforested areas planted with dipterocarpus seedlings inoculated with ectomycorrhizae and a non-reforested area at Chulalongkorn University Area, Saraburi Province, Thailand. Soil microarthropods were extracted with Berlese-Tullgren funnels from monthly collected soil samples from October 2014 to October 2015. The total abundance of soil microarthropods was highest in the 2-year dipterocarpus reforestation area (3,596 ± 227 ind./m2), followed by the 1-year reforestation area (2,989 ± 334 ind./m2) and the non-reforested area (2,496 ± 361 ind./m2), respectively (F = 1.988, df = 2, 73, p = 0.051). Mites (74-83%) and collembolans (15-21%) were the most abundant groups from the nine groups of collected soil microarthropods in all three areas. Symphylans, spirobilids, geophilomorphs and proturans were more abundant in the reforestation areas than the non-reforested area, while pseudoscorpions were most abundant in the non-reforested area. Detrito-fungivorous microarthropods were the most abundant guild in all three areas, followed by predatory arthropods and detritivore microarthropods. Diversity index of soil microarthropods at morphospecies level was lowest in the non-reforested area (H/ = 0.85) when compared to the 1-year reforestation area (H/ = 0.92) and the 2-year reforestation area (H/ = 0.93). Soil moisture was significantly different in three areas (F = 93.602, df = 2, 33, p &lt; 0.001), and was highest in the 2-year dipterocarpus reforestation area, followed by the 1-year reforestation area and the non-reforested area, respectively. However, soil microarthropods were positively correlated with soil moisture only in the non-reforested area (r = 0.752, p = 0.003). This study shows that increasing age progression of seedling affected several environmental factors, particularly soil moisture, which in turn relate to an increasing trend of the diversity and abundance of soil microarthropods.
DOI: 10.14457/cu.the.2015.414
title: Designing peptides with inhibitory activity on human papillomavirus 16 E1-E2 protein complex formation

description: Human papillomavirus 16 (HPV 16) is a DNA virus that is capable of infecting humans and causing cervical cancer. HPV16 E2 plays an important role in viral gene regulation and replication. Fujii et al 2003 reported that nine peptides screened by phage display technique, could bind to HPV16 E2. This work aims to predict the binding conformations and interactions between the dodecapeptides and HPV16 E2 as well as to design novel peptide inhibitors that are capable of binding to HPV16 E2 and disrupting the transcriptional regulator E1-E2 complex formation, using computational protein design techniques. ClusPro Web Server was used to dock these peptides to E2. The ranking of the predicted binding affinities of the peptides is consistent to that of the experimental results; and peptide4 is the best E2 binder. Therefore, small peptides were designed based on peptide4 (TWFWPYPYPHLP). Trp4 of peptide4 was mutated to His, Asn and Ser, Tyr6 of peptide4 was mutated to Lys, Asn and Arg. Five newly designed peptides that showed lower binding energy to HPV16 E2 than that of peptide4, were selected for in vitro experiments. The HPV16 E1 and E2 were expressed and purified in Escherichia coli. Enzyme- linked immunosorbent assay was used to test whether the designed peptides could bind to HPV16 E2 and disrupt the E1-E2 complex formation better than peptide4. Y6R, W4H_Y6R and W4H peptides could bind better to HPV16 E2 than peptide4 did. Moreover, these three peptides are more effectively inhibited E1-E2 complex formation than peptide 4.
DOI: 10.14457/cu.the.2015.415
title: Abundance and courtship behavior of a firefly Luciola sp. HK 1 at The Huai Hongkhrai Royal Development Study Center, Doi Saket District, Chiang Mai Province

description: Abundance and courtship behavior of Luciola sp. HK 1 at the Huai Hongkhrai Royal Development Study Center, Doi Saket District, Chiang mai Province were investigated from July 2014 to June 2015. Adults of the Luciola sp. HK 1 firefly were sampled along a 120-meter transect line nearby a creek. The numbers of adult firefly flashed were recorded by direct counting method using a counter. Then, an aerial net was used to catch all adult fireflies in each transect line section. The numbers of all adult fireflies caught and the numbers of adults Luciola sp. HK 1 caught were recorded and the indirect estimated numbers of individual of Luciola sp. HK 1 were calculated in the unit of individuals/ 40-meter section of transect line. The indirect estimated numbers of individual of Luciola sp. HK 1 were 2.57±0.76 in July, 2.77±0.64 in August, 2.64±0.84 in September, 3.68±0.83 in October, 4.90±0.99 in April, 4.40±0.80 in May, 4.30±0.86 in June; and adult Luciola sp. HK 1 was not found in November 2015 to March 2015. Firefly flash display peaks at about 55 minutes after first flash, so the recommended time for ecotourism is around 7:00-8:00 PM. To study of courtship behavior of a firefly Luciola sp., the study was conducted with adult fireflies in the laboratory during 6:00-8:00 PM by keeping a couple specimen of one adult male and one adult female fireflies in a clear square box with lid, then, started recording the moving and flashing of the couple specimen. The video was recorded in 3 phases; before the couple saw each other, during the period that the couple saw each other and the mating period between the couple specimen. It was found that there were 3 flashing patterns; male flash, female respond and mating flash patterns.
DOI: 10.14457/cu.the.2015.416
title: Structural geology in Amphoe Wang Nam Khiao area, Changwat Nakhon Ratchasima

description: Tectonic evolution in study area all along had been effected to bring about the apparent geological structure in Amphoe Wang Nam Khiao Area, where situated between the Sibumasu terrane, the Sukhothai-Chanthaburi terrane and the Indochina terrane. The rocks stratigraphy in study area in term of age form older to younger can be recognized to be five groups, including the Permian sedimentary rocks, the Permo-Triassic volcanic rocks, the Triassic Intrusive rocks in NE part of the study area, the Triassic hornblende granite and the Mesozoic in the Khorat group. Structural geology studies by measuring the orientations of apparent structure in outcrops such as foliations, bedding which plotted in stereonet diagrams and complied with stratigraphy, suggests two groups of trend. The first group includes the Permian sedimentary rocks, the Permo-Triassic volcanic rocks, some part of the Triassic intrusive rocks and the hornblende granite illustrated trend ENE-WSW to ESE-WNW and NE-SW. The second group consists of the Triassic intrusive rocks in SW part of the study area illustrated NW-SE orientation trends. Microstructures study further show evidence of brittle and ductile deformation mechanism. The orientations analysis of structural geology obtain and stratigraphy from the study area are indicated two deformations, as a result the former deformation (D1) generated first fold (F1) in ENE-WSW to ESE- WNW and NE-SW directions. The first fold was superimposed by the later deformation (D2) generated second fold (F2) in NW-SE direction. The resulting of two deformations caused structure like dome and basin apparent in Amphoe Wang Nam Khiao Area.
DOI: 10.14457/cu.the.2015.417
title: Taxonomy and systematics of terrestrial earthworm genera Amynthas Kinberg, 1867 and Metaphire Sims and Easton, 1972 in Thailand

description: Taxonomy and systematics of two main earthworm genera of the family Megascolecidae has been carried out and analyzed in Thailand. The previous reports confirmed the total number of 29 nominal species which 18 belonging to the genus Amynthas and 11 to the genus Metaphire. After making additional 123 collecting sites and carefully examining principal morphological characters such as the male pore area, spermathecae, genital markings, testes, seminal vesicles, prostate gland and intestinal caeca. The total number of 44 nominal species and three undescribed have been confirmed and reported. Of which, two are newly record to Thailand: Amynthas andersoni (Michaelsen, 1907) and A. corticis (Kinberg, 1867), while three are considered to be peregrine species. There are A. corticis (Kinberg, 1867), A. morrisi (Beddard, 1892) and A. gracilis (Kinberg, 1867). In additional, fourteen species have been described containing seven from each genus. The re-descriptions of the type species of the genus Amynthas, A. aeruginosus Kinberg, 1867 has also been written. The total number has finally been clarified as 26 and 18 species of the genera Amynthas and Metaphire respectively. The phylogenetic analysis using COI and ITS2 genes has been analyzed and using 27 operational taxonomic units (OTUs), consisting of 15 species of Amynthas and 12 species of Metaphire. The results were largely congruent with morphological identified species. However, some possible cryptic speciation has been noticed in Metaphire houlleti species complex, which probably contains at least three distinct species. Phylogenetic relationships between the two genera still have not been resolved clearly. The relations are not monophyly, but interpreted as polyphyly and/or paraphyly groups. However the closed relationships have been found in many cases such as Amynthas thakhantho and A. turris; Metaphire peguana and M. bahli which showing monophyletic relationships. The adding taxa and sampling localities and also genes may be interpreted the better distinct phylogenetic analysis.
DOI: 10.14457/cu.the.2015.418
title: All-cellulose nanocomposites film from sisal fiber

description: In this work, self-reinforced cellulose nanocomposite films were produced using cellulose and nanofiber from sisal fiber as matrix and reinforcement, respectively. Cellulose nanofiber was prepared via catalytic oxidation using TEMPO/ NaClO/ NaClO2 system. By mild mechanical treatment in water, oxidized celluloses could be disintegrated into individual cellulose nanofiber and utilized as nanofiller. A morphology of TEMPO-oxidized cellulose nanofiber was characterized through transmission electron microscopy (TEM), which revealed nanosized fibrils with diameters in the range of 10–20 nm and at least 1 µm in length. These cellulosic nanofibers were subsequently impregnated in dissolved cellulose matrix which was prepared by dissolving sisal fiber in lithium chloride/N,N-dimethylacetamide solvent. The effects of reinforcement content in all-cellulose nanocomposite films were examined in terms of morphology, mechanical properties, physical properties, and thermal properties. The crystallinity of the nanocomposite films was increased as the cellulose nanofiber content went up. Even though tensile strength of 0.5% composite film was reduced from 40 to 29 MPa, elongation at break was greatly increased from 11% to 37%. These results mean that the nanocomposite films were tougher than the neat cellulose film. In addition, the cellulose nanofiber led to an improvement in the thermal stability of the nanocomposite films, as evidenced by an increment of the onset of the degradation temperature. The hydrophilicity of the nanocomposite film was decreased with an increasing amount of cellulose nanofiber. The % water absorption of the nanocomposite film was reduced from 202% to 150% with the addition of 2% nanofiber.
DOI: 10.14457/cu.the.2015.419
title: Mesostructured Sr AND Ti mixed oxide catalysts for synthesis of fatty acid methyl esters

description: The objectives of this research were synthesis of mesostructured Sr and Ti mixed oxides (MSTs) via a self-assembly sol-gel combustion method using Pluronic P123 as a structure-directing agent, in order to use them as heterogeneous catalyst in transesterification of palm kernel oil (PKO) and methanol. Firstly, the effects of synthesis parameters on physicochemical properties of MSTs were studied. The results showed that the MST synthesized with molar ratio of citric acid: total metals at 1: 1, amount of Pluronic P123 at 0.17 mmol and calcination temperature at 600 °C exhibited good structural and textural properties. After that, the catalytic activity and stability of MST synthesized without citric acid (MST-0C-0.17P(600)) and MST synthesized with adding citric acid at molar ratio of citric acid: total metals 1: 1 (MST-1C-0.17P(600)) were comparatively studied in transesterification. Although the reaction over MST-1C-0.17P(600)) required the temperature higher than that over MST-0C-0.17P(600)), to achieve the fatty acid methyl esters (FAME) yield of 99.8 wt%, the MST-1C-0.17P(600)) showed superior chemical stability compared to the MST-0C-0.17P(600)) in the transesterification. Finally, the results from in situ FTIR showed that MST-1C-0.17P(600)) had Lewis acid sites and 4 types with different strength of basic sites. Furthermore, in situ FTIR results provided the information about the nature of adsorbed methanol and ethyl propionate species on the active sites of MST-1C-0.17P(600). Moreover, the reaction mechanism for the transesterification using the MST-1C-0.17P(600) as catalyst was proposed.
DOI: 10.14457/cu.the.2015.420
title: Solubility enhancement of pinostrobin by complexationwith β-cyclodextrin and its derivatives

description: Pinostrobin (PNS) is one of the important flavonoids and can be abundantly found in the rhizomes of fingerroot Boesenbergia rotrunda and galangal Alpinia galangal and A. officinarum. Similar to other flavonoids, PNS has an extremely low water solubility that limits its use in pharmaceutical applications. Beta-cyclodextrin (βCD) and its derivatives, heptakis (2,6-di-O-methyl)-βCD (2,6-DMβCD) and 2-hydroxypropyl-βCD (HPβCD), can enhance the stability and solubility of low-soluble guest molecules. In the present work, computational and experimental studies were applied to investigate the dynamics and stability of PNS inclusion complexes with βCD and its derivatives, 2,6-DMβCD and HPβCD. From molecular dynamics (MD) study, PNS was able to form complexes with all βCDs by either the chromone (C-PNS) or phenyl (P-PNS) ring dipping towards the cavity. According to calculated binding free energies, the stability of the different PNS/βCDs complexes was in the order of 2,6-DMβCD &gt; HPβCD &gt; βCD in good agreement with the experimental stability constants. The AL-type diagram exhibited the formed inclusion complexes with the 1:1 molar ratio. The inclusion complexes were successfully prepared by freeze-drying method and characterized by DSC and 2D-ROESY NMR techniques. Steered MD and 2D-ROESY results revealed the both binding modes of PNS favorably occupied inside the cavity of βCD derivatives, whilst C-PNS likely preferred to interact with the parental βCD. All PNS/βCDs complexes had a higher dissolution rate than the free PNS. Both PNS and its complexes significantly exerted a lowering effect on the IL-6 secretion in LPS-stimulated macrophages and showed a moderate cytotoxic effect against HeLa and MCF-7 cancer cell lines in vitro.
DOI: 10.14457/cu.the.2015.421
title: Preparation of rigid polyurethane foams catalyzed by delayed action catalyst derived from copper-amine complexes and carboxylic acid

description: In this research, a delayed action catalyst system is used in the preparation of rigid polyurethane foams. The delayed action catalysts were synthesized from reaction between copper-amine complexes and carboxylic acids in ethylene glycol as a solvent to give the salts of copper-amine complex and carboxylic acid. Copper-amine complexes used in this study were Cu(OAc)2(en)2 and Cu(OAc)2(trien) (where en = ethylenediamine and trien = triethylenetetramine). Carboxylic acids used were salicylic acid (Sal) and acetic acid (Ac). Copper-amine complex/carboxylic acid salts, namely Cu(OAc)2(en)2/Sal, Cu(OAc)2(en)2/Ac and Cu(OAc)2(trien)/Sal were studied. UV-Visible spectroscopy and IR spectroscopy were used to characterize the structure of copper-amine complex/salicylic acid salts. The reaction times in the foam formation, physical and mechanical properties of foams are studied. When only copper-amine complex was used as a catalyst, the foam formation was fast. However, there was a problem with the fast gel time which resulted in difficulty in the foam processing. This problem could be solved by using copper-amine complexes/carboxylic acid salts as catalysts, which gave longer gel time while the tack free time was not too long. Cu(OAc)2(en)2/Sal and Cu(OAc)2(trien)/Sal at the mole ratios of 1:1 and 1:0.5, respectively were suitable catalysts, which had gel time about 60 seconds while tack free time was not too slow when compared with commercial catalyst (DMCHA). The gel time of 60 sec was long enough for pouring the mixture of foam formulation into a mold.
DOI: 10.14457/cu.the.2015.422
title: Effects of emulsifiers on mixing properties, viscoelastic properties and stability of zein-starch doughs

description: Zein, maize protein, has ability to form viscoelastic dough but requires mixing above room temperature (35oC) and has inferior dough properties compared to wheat dough. The objective of this research was to study effect of emulsifiers on zein-starch dough’s properties, including the mixing properties, viscoelastic properties, and dough stability. Corn starch (90%, w/w of flour), zein (10%, w/w of flour), emulsifier (4-6% PATCO-3™ or 2-4% sodium stearoyl lactilates (SSL), w/w of flour) and water (optimum %) were mixed at 35oC and 28oC into dough. Farinograph results revealed that emulsifier addition decreased optimum water amount and prolonged mixing time. SSL dough exhibited lower water amount and less mixing time than PATCO-3™ dough. Dough with emulsifier showed lower storage modulus G’ transition temperature (T of G’ transition) compared to the control. More pronounced depression in T of G’ transition was shown by addition of SSL than PATCO-3™ and the higher the emulsifier concentration, the greater the decrease obtained. Small deformation test showed that the phase angle value decreased with emulsifier incorporation, indicating enhancement of its elastic characteristic. Complex modulus, storage modulus, and viscous modulus values also increased, recognized as higher dough strength. Those were more obvious in SSL dough than PATCO-3™ dough. Those trends also were shown for dough mixed at 28oC and 35oC, indicating zein could be functionalized at below room temperature through decreasing in T of G’ transition. FTIR results exhibited that PATCO-3™ dough had better stability than SSL dough, as shown by relatively higher and constant of β-sheet secondary structure amount during storage for 15 min at room temperature (25-28oC). More apparent effect was seen in dough mixed at 35oC than at 28oC. Comparison with the control was not possible due to severe phase separation. The results of this study suggested that PATCO-3™ and SSL might help to form viscoelastic zein-starch dough at room temperature. PATCO-3™ had more positive effect on G’ transition temperature, viscoelastic properties and dough stability. SSL addition even though resulted in lower G’ transition temperature and more improved viscoelastic nature, the dough was unstable during storage. Thus, structural improvement in zein cannot be attributed only by the effect of G’ transition but also the interaction between zein and emulsifier. The proper balance between hydrophobic and hydrophilic parts of emulsifier might be required to get optimum decreased T of G’ transition, viscoelastic properties, and stability of zein-starch dough.
DOI: 10.14457/cu.the.2015.423
title: Detection of glucose by silver nanoplates paper-based sensor

description: Silver nanoplates (AgNPls) were employed to detect a low level of hydrogen peroxide (H2O2) and glucose based on the decomposition of AgNPls. A new AgNPls-modified paper was fabricated by depositing AgNPls on a filter paper coated with (3-mercaptopropyl) trimethoxysilane (MPTMS). For H2O2 detection, the color of AgNPls-modified paper was bleached due to the oxidation of AgNPls by H2O2, when immersed in H2O2 solution. It could be used to detect H2O2 in the concentration range from 0.7 to 3.0 mM by naked eyes detection and the quantitative data was measured via ImageJ software. For glucose detection, glucose oxidase was added into urine samples to react with glucose and generate H2O2. AgNPls-paper strip was prepared and used in this method. The standard addition method and sample dilution were performed to overcome the matrix effect from human urine. Chloride, sulfate and phosphate ions in urine samples were eliminated by adding AgNO3 into the urine sample solutions to form precipitates. In the determination of glucose in urine samples, the dynamic linear range was in the range from 0 to 1.4 mM. The recovery of glucose added to urine samples in the range of 94 to 105% was obtained with the relative standard deviation of the results in the range of 5.9–21.4%. The paper-based method could be used for glucose detection in human urine with acceptable accuracy and precision.
DOI: 10.14457/cu.the.2015.424
title: Rearrangement of epoxides by transition metal salts in ionic liquid

description: A new and efficient system for the rearrangement of aryl epoxides by nickel complexes in imidazolium ionic liquids has been developed. Types of nickel complexes, ionic liquids, reaction temperature and reaction time were optimized. The rearrangement of styrene oxide with NiBr2·3H2O and anhydrous NiBr2 in 1-hexyl-3-methylimidazolium bromide ([hmim][Br]) at 120oC for 15 min provided 76 and 84% yield of phenylacetaldehyde, respectively. Applications on the rearrangements of aryl-substituted epoxides were fruitfully achieved yielding desired products in moderate to high yield and excellent selectivity via hydride migration. The activity of the reused NiBr2.3H2O/[hmim][Br] was tested in subsequent runs without addition of fresh catalyst for five runs on α-methylstyrene oxide. However, the reuse of NiBr2.3H2O/[hmim][Br] with styrene oxide gave unsatisfactory result. Anhydrous NiBr2 could be used to solve this problem. Moreover, the aids of microwave for the rearrangement of styrene oxide with anhydrous NiBr2 in [hmim][Br] at 90 oC for 5 min provided 85% yield of the target product. Very good reusability of the recovered ionic liquid without significant loss of the activity was observed within three subsequent runs.