DOI: 10.57715/unr/qkldvr
title: Experimental and analysed data from the characterization of bromodomain factor of Trypanosoma cruzi (TcBDF6)

description: Introduction Bromodomains regulate gene expression in trypanosomatids by recognizing acetylated lysine residues, primarily in histones. Known as "reader" epigenetic proteins, they act as scaffolds to assemble macromolecular complexes that interact with chromatin. In T. cruzi, genes are organized and transcribed in a polycistronic manner, and expression depends on the degree of chromatin compaction. In this Dataset, we characterized one of the 8 bromodomains, TcBDF6. We used CRISPR/Cas9 to generate the mutant strain Dm28cBDF6-/-. This strain enabled us to investigate the bromodomain, revealing a highly interesting phenotype characterized by decreased in vitro infectivity and no intracellular replication. Dataset Content and File Organization This dataset contains the experiments corresponding to the raw data and analyzed data with the strains: DM28c (wild strain) BDF6-/+ (hemizygous mutant strain) BDF6-/- (homozygous mutant strain) BDF6-/- pBDF6 (complemented or addback strain) In this dataset, there are three parts ordered in three general folders: 1. Description of the phenotype from the mutant strain Epimastigotes growth curves: This folder contains two files, one from raw data of the Growth curve RawData_Growth curve epimastigotes.txt and the other file AnalyzedData_Growth_curve_epimastigotes.pzf with the graphics, statistics, and tests. Dormancy assays: This folder contains two files, one from raw data of the dormancy assay  RawData_Dormancy_assay.txt and the other named Dormancy_assay_graphs.pzfx with the graphics, statistics, and tests. Quantification of the area of epimastigotes: this section has raw data of the quantification of areas measured and with the file format  .txt  for each strain, and we also add the file Quantification_analyzed_data_Areas_of_epimastigotes.pzfx. Expansion microscopy from epimastigotes and trypomastigotes: in this section, there are files from expansion microscopy of epimastigotes in the file format .czi (confocal microphotographs, stained with NHS-ester and DAPI) Cell cycle analysis of epimastigotes by flow cytometry: analyzed data of cell_cycle.pzfx  Oxidative Stress Assays: analyzed data from oxidative stress assay in the file oxidative_stress.pzfx 2. In vitro Cell infection with T.cruzi strains Percentage of infected cells and amount of amastigotes in the cells: the raw data file is quantification of infection.tab and the analyzed file is Analyzedata_invitro_infections.pzfx  Colocalization of amastigotes and lysosomes: raw data from this assay with the file format .oib 3. Mice infection In this section, we exposed the results from Parasite burden in vivo,  detection of Parasite Burden in tissues, qPCR quantification of parasite load in blood and Antibody quantification by ELISA in mouse sera. All the results are shown in only one file named In_vivo_infections_BDF6.pzfx Each assay folder contains: Raw data (format file: e.g., .csv, .xls, .oib or .czi) Analyzed data including graphical representations and statistical analyses (format file: e.g., .pzf) Value of the dataChagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects millions worldwide, with increasing urban cases emerging beyond Latin America. Current treatments rely on two drugs: benznidazole and nifurtimox, which have limitations, especially in chronic cases. This study highlights the critical role oTcBDF6, a bromodomain protein, in the infection phenotype of T. cruzi. Experimental findings reveal that intracellular amastigotes fail to replicate effectively when host cells have reduced or absent TcBDF6 expression. By identifying TcBDF6 as a key regulator of gene expression during the amastigote stage, this research enhances our understanding of T. cruzi biology. The findings provide a foundation for developing innovative therapeutic strategies to combat Chagas disease.