DOI: 10.5061/dryad.w3r2280xw
title: Data from: Genetic analysis of museum samples suggests temporal stability in the Mexican nonbreeding distribution of a neotropical migrant

description: Seasonal migration is highly labile from an evolutionary perspective and
 known to rapidly evolve in response to selective pressures. However,
 long-distance migratory birds rely partially on innate genetic programs
 and may be constrained in their ability to alter their migratory behavior.
 We take advantage of recent advances in our ability to genotype historical
 DNA samples to examine the temporal stability of migratory connections
 between breeding and nonbreeding populations (i.e., migratory
 connectivity) and population-level nonbreeding distributions in the
 Wilson’s warbler (Cardellina pusilla), a long-distance migratory songbird.
 By assigning historical and contemporary samples collected across the
 nonbreeding range to genetically distinct breeding clusters, we suggest
 that broad-scale population-level nonbreeding distributions within this
 species have remained largely consistent within Mexico from the mid-1900s
 to the present day. These findings support the idea that the nonbreeding
 distributions of long-distance migrants may remain stable over long time
 scales, even in the face of rapid environmental change.
description: # Data from: Genetic analysis of museum samples reveals historical
 wintering distribution of a neotropical migrant
 [https://doi.org/10.5061/dryad.w3r2280xw](https://doi.org/10.5061/dryad.w3r2280xw) This repository contains data generated for the following paper: Polich, A., C.M. Bossu, C. Rayne, A. Carpenter, G. Rodríguez Otero, S. Gómez Villaverde, F. Rodríguez Vásquez, B.E. Hernández-Baños, J. McCormack, K. Ruegg, and S.P. Turbek. Genetic analysis of museum samples reveals historical wintering distribution of a neotropical migrant. ## Description of the data and file structure #### Reference\_Breeding\_WIWA\_Rubias\_Input.txt This file contains genotypes at 96 loci for 407 contemporary Wilson's warblers with known breeding locations taken from Ruegg et al. (2014). These birds served as reference samples when carrying out population assignment with the *rubias* R package. 'sample_type' indicates whether the sample is a reference (i.e., known origin) or mixture (i.e., unknown origin) sample, 'repunit' indicates the reporting unit that the individual belongs to, and 'collection' indicates the name of the population that the individual is from (for reference samples). Missing data are coded as 0. #### Contemporary\_WIWA\_Rubias\_Input.txt This file contains genotypes at 96 loci for the 131 newly genotyped contemporary Wilson's warblers collected from 1998-2020 across Mexico and Honduras. This file was read into *rubias* for population assignment of the contemporary samples. 'sample_type' indicates whether the sample is a reference (i.e., known origin) or mixture (i.e., unknown origin) sample. Missing data are coded as NA. #### Contemporary\_WIWA\_Metadata.csv This file contains metadata for the 131 newly genotyped contemporary Wilson's warblers collected from 1998-2020 across Mexico and Honduras. 'State' refers to the state or department where each individual was sampled. #### Historical\_WIWA\_Rubias\_Input.txt This file contains genotypes at 96 loci for the 49 historical Wilson's warblers collected across Mexico from 1934-1964. This file was read into *rubias* for population assignment of the historical samples. 'sample_type' indicates whether the sample is a reference (i.e., known origin) or mixture (i.e., unknown origin) sample. Missing data are coded as NA. #### Historical\_WIWA\_Metadata.csv This file contains metadata for the 49 historical Wilson's warblers collected across Mexico from 1934-1964. State refers to the state or department where the individual was sampled. #### Ruegg-et-al-2014\_Contemporary\_Assignments.txt This file contains population assignment information for 239 contemporary Wilson's warblers from Ruegg et al. (2014) that were sampled across the wintering grounds (Mexico, Nicaragua, El Salvador, Belize, Costa Rica, and Honduras). 'State' refers to the state or department where the individual was sampled. For each individual, the file indicates the probability of assignment to the Western Boreal ('AK2Alberta'), Coastal California ('CoastalCA'), Eastern ('Eastern'), Pacific Northwest ('PacNorthwest'), Basin Rockies ('RockyMtn'), and California Sierra ('Sierra') breeding clusters.