DOI: 10.22002/064p0-gh146
title: AimB depletion control w/ xylose

description: Raw data files of AimB depletion control w/ xylose
description: <strong>Tilt Series Date:</strong> 2009-09-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> The AimB_depletion strain was constructed by John Werner, data collected by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity</p>
  zla2009-09-21-35_slicer10066.jpg: Caulobacter crescentus .  nested vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/rawdata/</p>09sep21a_AimBxyl_053.mrc,  Tilt Series (Pixel Size 1.192 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/rawdata/09sep21a_AimBxyl_053.mrc"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_053_part.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/3dimage_10382/09sep21a_AimBxyl_053_part.rec"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_0531.tif,  file associated to 3D Image #10382, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/file_2845/09sep21a_AimBxyl_0531.tif"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_0532.tif,  file associated to 3D Image #10382, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/file_2846/09sep21a_AimBxyl_0532.tif"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_0533.tif,  file associated to 3D Image #10382, 780.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/file_2847/09sep21a_AimBxyl_0533.tif"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_053.tif,  file associated to 3D Image #10382, 813.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-35/file_2848/09sep21a_AimBxyl_053.tif"
        > <i class="download icon"></i> Download </a></p>