cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14268326.v1
title: Identification and sequence analysis of prolactin receptor and its differential expression profile at various developmental stages in striped hamsters

description: Prolactin (PRL) plays critical roles in regulation of biological functions with the binding of specific prolactin receptor (PRLR). Revealing the expression patterns of PRLR at different developmental stages is beneficial to better understand the role of PRL and its mechanism of action in striped hamsters. In this study, the cDNA sequence of PRLR (2866-base-pairs) was harvested from the pituitary of mature female striped hamsters (Cricetulus barabensis) that contains an 834-base-pair 5′-untranslated region (1-834 bp), a 1848-base-pair open reading frame (835-2682 bp), and a 184-base-pair 3′-untranslated region (2683-2866). The 1848-base-pair open reading frame encodes a mature prolactin-binding protein of 592 amino acids. In the mature PRLR, two prolactin-binding motifs, 12 cysteines, and five potential Asn-linked glycosylation sites were detected. Our results showed that the PRLR mRNA quantity in the hypothalamus, pituitary, ovaries, or testis was developmental-stage-dependent, with the highest level at sub-adult stage and the lowest level at old stage. We also found that PRLR mRNAs were highest in pituitary, medium level in hypothalamus, and lowest in ovaries or testis. PRLR mRNAs were significantly higher in males than in females, except in the hypothalamus and pituitary from 7-week-old striped hamsters. Moreover, the PRLR mRNAs in the hypothalamus, pituitary, and ovaries or testis were positively correlated with the expression levels of GnRH in the hypothalamus. These results indicated that the PRLR has conserved domain in striped hamster, but also possesses specific character. PRLR has multiple biological functions including positively regulating reproduction in the striped hamster.
DOI: 10.6084/m9.figshare.12171198.v1
title: Tissue fusion technology versus suture and staple in porcine bowel anastomosis: an in vivo study

description: The aim of this study was to make a comparison between the tissue fusion technique and conventional methods for sealing bowel anastomosis. Eighteen female domestic pigs (Suidae, Sus) were used in our study. Tissue-fused anastomoses (LigaSure groups) were made in 13 animals (5 anastomoses per animal), which were subdivided into 4 groups according to different manufacturing settings: “LigaSure-L-1” and “LigaSure-L-2”, with low energy output level with 1 or 2 device-activated tissue sealing times, and “LigaSure-M” and “LigaSure-H”, with medium or high energy output level. As controls, automatically stapled (GIA group) and hand-sewn (suture group) anastomoses were utilized in 3 and 2 animals, respectively. There was no statistical difference in the overall leakage rate between the GIA group (6.7%) and the LigaSure groups (15%) (P=1.000). There was less proliferating epithelium covering the anastomosis gap in the LigaSure groups compared with the other two groups. The gap between the two extremities of muscular layers of the anastomosis in the LigaSure groups was filled with collagen fibers. More proliferating cell nuclear antigen (PCNA)-positive cells were found in the anastomoses of the LigaSure groups compared with the other two groups (P=0.010). Our results showed that the tissue fusion technology was a feasible and safe method for anastomoses.
DOI: 10.6084/m9.figshare.21608186
title: Chemotherapy plus panitumumab/cetuximab versus chemotherapy plus bevacizumab in wild-type KRAS/RAS metastatic colorectal cancer: a meta-analysis

description: It remains controversial which targeted monoclonal antibodies combined with chemotherapy can provide better efficacy in wild-type KRAS/RAS metastatic colorectal cancer (mCRC) patients. Therefore, we used this meta-analysis to assess the latest evidence of clinical outcomes. We systematically searched PubMed, Web of Science, Cochrane Library and Embase databases for eligible studies published from database inception to May 2022. RevMan 5.4 was used to conduct the meta-analysis. 11 RCTs involving a total of 3575 patients were included. Meta-analysis showed that EGFR inhibitors significantly prolonged the overall survival (OS) [HR = 0.83, 95%CI (0.73, 0.94), <i>P</i> = 0.003] and overall response rate (ORR) [RR = 1.11, 95%CI (1.05, 1.18), <i>P</i> = 0.0003] compared to VEGF inhibitors in wild-type KRAS/RAS mCRC patients, but no significant difference in progression-free survival (PFS) [HR = 0.96, 95%CI (0.87, 1.07), <i>P</i> = 0.50]. In subgroup analysis, the survival benefit of EGFR inhibitors was limited to first-line treatment. Our study showed that EGFR inhibitors were superior to VEGF inhibitors in wild-type KRAS/RAS mCRC patients, especially in patients with first-line treatment. However, subsequent large sample, multi-center RCTs are needed to further verify our conclusions.
DOI: 10.6084/m9.figshare.7976906
title: Metastable DNA methylation sites associated with longitudinal lung function decline and aging in humans: an epigenome-wide study in the NAS and KORA cohorts

description: DNA methylation is an epigenetic regulator of gene transcription, which has been found to be both metastable and variable within human cohort studies. Currently, few studies have been done to identify metastable DNA methylation biomarkers associated with longitudinal lung function decline in humans. The identification of such biomarkers is important for screening vulnerable populations. We hypothesized that quantifiable blood-based DNA methylation alterations would serve as metastable biomarkers of lung function decline and aging, which may help to discover new pathways and/or mechanisms related to pulmonary pathogenesis. Using linear mixed models, we performed an Epigenome Wide Association Study (EWAS) between DNA methylation at CpG dinucleotides and longitudinal lung function (FVC, FEV<sub>1</sub>, FEF<sub>25–75%</sub>) decline and aging with initial discovery in the Normative Aging Study, and replication in the Cooperative Health Research in the Region of Augsburg cohort. We identified two metastable epigenetic loci associated with either poor lung function and aging, cg05575921 (<i>AHRR</i> gene), or lung function independently of aging, cg06126421 (<i>IER3</i> gene). These loci may inform basic mechanisms associated with pulmonary function, pathogenesis, and aging. Human epigenomic variation, may help explain features of lung function decline and related pathophysiology not attributable to DNA sequence alone, such as accelerated pulmonary decline in smokers, former smokers, and perhaps non-smokers. Our EWAS across two cohorts, therefore, will likely have implications for the human population, not just the elderly.
DOI: 10.6084/m9.figshare.7976906.v1
title: Metastable DNA methylation sites associated with longitudinal lung function decline and aging in humans: an epigenome-wide study in the NAS and KORA cohorts

description: DNA methylation is an epigenetic regulator of gene transcription, which has been found to be both metastable and variable within human cohort studies. Currently, few studies have been done to identify metastable DNA methylation biomarkers associated with longitudinal lung function decline in humans. The identification of such biomarkers is important for screening vulnerable populations. We hypothesized that quantifiable blood-based DNA methylation alterations would serve as metastable biomarkers of lung function decline and aging, which may help to discover new pathways and/or mechanisms related to pulmonary pathogenesis. Using linear mixed models, we performed an Epigenome Wide Association Study (EWAS) between DNA methylation at CpG dinucleotides and longitudinal lung function (FVC, FEV<sub>1</sub>, FEF<sub>25–75%</sub>) decline and aging with initial discovery in the Normative Aging Study, and replication in the Cooperative Health Research in the Region of Augsburg cohort. We identified two metastable epigenetic loci associated with either poor lung function and aging, cg05575921 (<i>AHRR</i> gene), or lung function independently of aging, cg06126421 (<i>IER3</i> gene). These loci may inform basic mechanisms associated with pulmonary function, pathogenesis, and aging. Human epigenomic variation, may help explain features of lung function decline and related pathophysiology not attributable to DNA sequence alone, such as accelerated pulmonary decline in smokers, former smokers, and perhaps non-smokers. Our EWAS across two cohorts, therefore, will likely have implications for the human population, not just the elderly.
DOI: 10.6084/m9.figshare.24235270
title: Mesozoic evolution of large-scale drainage systems in the Indochina Block: evidence from paleomagnetic and U-Pb geochronological constraints

description: Table S5.
DOI: 10.6084/m9.figshare.24235270.v1
title: Mesozoic evolution of large-scale drainage systems in the Indochina Block: evidence from paleomagnetic and U-Pb geochronological constraints

description: Table S5.
DOI: 10.6084/m9.figshare.24235276
title: Mesozoic evolution of large-scale drainage systems in the Indochina Block: evidence from paleomagnetic and U-Pb geochronological constraints

description: Table S4.
DOI: 10.6084/m9.figshare.24235276.v1
title: Mesozoic evolution of large-scale drainage systems in the Indochina Block: evidence from paleomagnetic and U-Pb geochronological constraints

description: Table S4.
DOI: 10.6084/m9.figshare.14268156
title: Pharmacological inhibition of mTORC1 activity protects against inflammation-induced apoptosis of nucleus pulposus cells

description: Lumbar disc herniation is a common disease characterized by the degeneration of intervertebral discs (IVDs), accompanied by imbalance of metabolic and inflammatory homeostasis. Current studies establish that IVD degeneration is induced by increased apoptosis of nucleus pulposus (NP) cells. However, the underlying mechanisms of NP cell survival/apoptosis are not well elucidated. Here, we reveal a novel mechanism by which mTORC1 signaling controls NP cell survival through regulating metabolic homeostasis. We demonstrated that hyperactivated mTORC1 activity induced by inflammatory cytokines engenders the apoptosis of NP cells, whereas pharmacological inhibition of mTORC1 activity promotes NP cell survival. Using an integrative approach spanning metabolomics and biochemical approaches, we showed that mTORC1 activation enhanced glucose metabolism and lactic acid production, and therefore caused NP cell apoptosis. Our study identified mTORC1 in NP cells as a novel target for IVD degeneration, and provided potential strategies for clinical intervention of lumbar disc herniation.
DOI: 10.6084/m9.figshare.6933806.v1
title: Additional file 7: of Dealing with the adaptive immune system during de novo evolution of genes from intergenic sequences

description: Sample designations for transcriptome samples and read count overview. (XLSX 59 kb)
DOI: 10.6084/m9.figshare.22132338
title: Research Status of SARS-CoV-2 on Cardiovascular System Injury in Children

description: Abstract In December 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began to break out in the Hubei Province of China. At present, the epidemic situation in the world continues and the number of confirmed cases is increasing every day. A recent review showed that children under the age of ten years make up about 1% of the infected population, which cannot be ignored. Studies have shown that after SARS-CoV-2 infection children can show clinical symptoms of cardiovascular system damage in addition to typical respiratory symptoms. This article mainly discusses the possible damage of SARS-CoV-2 to children's cardiovascular system and related mechanisms.
DOI: 10.6084/m9.figshare.7452443.v1
title: Hydrogen Storage Properties and Reactive Mechanism of LiBH4/Mg10YNi-H Composite

description: The Mg10YNi alloy was hydrogenated and then coupled with LiBH4 to form LiBH4/Mg10YNi-H reactive hydride composite. The results indicate that thermal dehydrogenation stability of LiBH4 can be remarkably reduced by combining with Mg10YNi hydride. The starting and ending temperatures for hydrogen desorption from the LiBH4/Mg10YNi-H composite are approximately 275 and 430 ºC, respectively. Dehydrogenation of the LiBH4/Mg10YNi-H composite proceeds mainly in two steps with a total reaction of 12LiBH4 + 2.5Mg10YNiH20 → 24Mg + MgNi2.5B2 + 2.5YB4 + 12LiH + 43H2. After rehydrogenation at 450 ºC under 9 MPa hydrogen pressure, the LiBH4/Mg10YNi-H composite starts to release hydrogen around 260 ºC, and as much as approximately 5.2 wt.% of hydrogen can be desorbed during the second dehydrogenation process.
DOI: 10.6084/m9.figshare.13219326
title: Additional file 3 of Integrated analysis of lncRNAs and mRNAs reveals key trans-target genes associated with ETEC-F4ac adhesion phenotype in porcine small intestine epithelial cells

description: Additional file 3. Total number of identified isoforms and DE-lncRNA.
DOI: 10.6084/m9.figshare.24235320
title: Characterization of the complete mitochondrial genome and phylogenetic analysis of bean thrips <i>Megalurothrips usitatus</i> (Bagnall, 1913) (Thysanoptera: thripidae)

description: <i>Megalurothrips usitatus</i> (Bagnall, 1913) (Thysanoptera: Thripidae) is a widely distributed pest in Asia that primarily affects the production of snap beans and cowpea. The complete mitochondrial genome of <i>Megalurothrips usitatus</i> has been sequenced and annotated in this study, which is 17,209 bp long and contains 13 protein-coding genes (PCGs), two rRNAs, and 22 tRNA genes. Most of the protein-coding genes (PCGs) start with ATG except <i>ND</i>4 using TTG. Meanwhile, eight PCGs stop with TAA, four PCGs have an incomplete stop codon, and the gene <i>Cytb</i> ends with TAG. Phylogenetic analysis showed that <i>M. usitatus</i> is closely related to <i>Frankliniella intonsa</i> and <i>F. occidentalis</i>, providing a basis for the study of the mitochondrial evolution of Thripinae.
DOI: 10.6084/m9.figshare.24235320.v1
title: Characterization of the complete mitochondrial genome and phylogenetic analysis of bean thrips <i>Megalurothrips usitatus</i> (Bagnall, 1913) (Thysanoptera: thripidae)

description: <i>Megalurothrips usitatus</i> (Bagnall, 1913) (Thysanoptera: Thripidae) is a widely distributed pest in Asia that primarily affects the production of snap beans and cowpea. The complete mitochondrial genome of <i>Megalurothrips usitatus</i> has been sequenced and annotated in this study, which is 17,209 bp long and contains 13 protein-coding genes (PCGs), two rRNAs, and 22 tRNA genes. Most of the protein-coding genes (PCGs) start with ATG except <i>ND</i>4 using TTG. Meanwhile, eight PCGs stop with TAA, four PCGs have an incomplete stop codon, and the gene <i>Cytb</i> ends with TAG. Phylogenetic analysis showed that <i>M. usitatus</i> is closely related to <i>Frankliniella intonsa</i> and <i>F. occidentalis</i>, providing a basis for the study of the mitochondrial evolution of Thripinae.
DOI: 10.6084/m9.figshare.24235323
title: The complete mitochondrial genome of the rice blast fungus <i>Pyricularia oryzae</i> Cavara 1892 strain Guy11 and phylogenetic analysis

description: The complete mitochondrial genome of <i>Pyricularia oryzae</i> Cavara 1892 strain Guy11 is 34,865 bp in length (GenBank accession number OP095391), containing 29 tRNA genes, 2 rRNA genes, and 15 protein-coding genes (PCGs). The gene order and orientation are novel compared to other Sordariomycetes species with sequenced mitogenomes in the GenBank database. Phylogenetic analysis suggests that <i>P. oryzae</i> Guy11 and 19 other Sordariomycetes species form a monophyletic group. The complete mitochondrial sequence of <i>P. oryzae</i> Guy11 will be a valuable resource for species identification, population genetics, phylogenetics, and comparative genomics studies in Sordariomycetes and Magnaporthales.
DOI: 10.6084/m9.figshare.24235323.v1
title: The complete mitochondrial genome of the rice blast fungus <i>Pyricularia oryzae</i> Cavara 1892 strain Guy11 and phylogenetic analysis

description: The complete mitochondrial genome of <i>Pyricularia oryzae</i> Cavara 1892 strain Guy11 is 34,865 bp in length (GenBank accession number OP095391), containing 29 tRNA genes, 2 rRNA genes, and 15 protein-coding genes (PCGs). The gene order and orientation are novel compared to other Sordariomycetes species with sequenced mitogenomes in the GenBank database. Phylogenetic analysis suggests that <i>P. oryzae</i> Guy11 and 19 other Sordariomycetes species form a monophyletic group. The complete mitochondrial sequence of <i>P. oryzae</i> Guy11 will be a valuable resource for species identification, population genetics, phylogenetics, and comparative genomics studies in Sordariomycetes and Magnaporthales.
DOI: 10.6084/m9.figshare.13219332
title: Additional file 5 of Integrated analysis of lncRNAs and mRNAs reveals key trans-target genes associated with ETEC-F4ac adhesion phenotype in porcine small intestine epithelial cells

description: Additional file 5. DE-lncRNAs and their cis-target genes.
DOI: 10.6084/m9.figshare.22132533
title: WeChat-Assisted Preoperative Health Education Reduces Burden of Care on Parents of Children with Simple Congenital Heart Disease: a Prospective Randomized Controlled Study

description: Abstract Objective: The purpose of this study was to explore the clinical effect of preoperative health education based on the WeChat platform for parents of children with simple congenital heart disease. Methods: In this study, participants were randomly divided into an intervention group (WeChat group, n=40) and a control group (leaflet group, n=40) in our center. All parents were required to complete the Family Caregiver Task Inventory (FCTI) on the first visit and the Zarit Burden Interview (ZBI) and FCTI before the operation. Clinical, family and relevant data from children and parents were collected and subsequently analyzed. Results: Before the operation, the FCTI score and the ZBI score in the WeChat group were significantly lower than those in the control group (P=0.010, P=0.027, respectively). Compared to the FCTI score on the first visit, the preoperative status score was significantly lower in the WeChat group (P=0.008). The rate of loss to follow-up and complications in the WeChat group was also significantly lower than in the control group (P=0.003). Conclusion: Preoperative health education assisted by the WeChat platform for parents of children with simple congenital heart disease can effectively improve the parents' care ability and reduce the burden of care, preoperative complications and the rate of loss to follow-up.
DOI: 10.6084/m9.figshare.12171213
title: MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL

description: Systemic lupus erythematosus (SLE) is an autoimmune disease associated with an aberrant activation of immune cells partly due to the dysfunction of cytokines such as type I interferons (IFNs). Long non-coding RNA MALAT1 has been found to play a pathogenic role in SLE; however, the underlying mechanisms are still poorly understood. Bioinformatics analysis showed the up-regulation of type I IFN downstream effectors OAS2, OAS3, and OASL (OAS-like) in CD4+ T cells, CD19+ B cells, and CD33+ myeloid cells in patients with active SLE compared to healthy participants. In this study, peripheral blood mononuclear cells (PBMCs), CD19+ B, and CD4+ T cells were isolated from active SLE patients and healthy participants. PCR was performed to quantify MALAT1, OAS2, OAS3, and OASL expression in immune cells. MALAT1, OAS2, OAS3, and OASL were knocked down in CD4+ T cells to investigate the regulatory effect of MALAT1 on the effectors and their involvement in type I IFNs-mediated inflammation. Results showed higher OAS2, OAS3, and OASL expression in active SLE patients. MALAT1 expression was positively correlated to OAS2, OAS3, and OASL expression in CD19+ B or CD4+ T cells. MALAT1 knockdown decreased OAS2, OAS3, and OASL expression. Treatment with IFN-α-2a increased the expression of TNF-α, IL-1β, and IFN-α in CD4+ T cells. However, knockdown of MALAT1, OAS2, OAS3, and OASL alone inhibited the effect of IFN-α-2a on TNF-α and IL-1β. This study suggested the involvement of MALAT1 in type I IFNs-mediated SLE by up-regulating OAS2, OAS3, and OASL.
DOI: 10.6084/m9.figshare.14268156.v1
title: Pharmacological inhibition of mTORC1 activity protects against inflammation-induced apoptosis of nucleus pulposus cells

description: Lumbar disc herniation is a common disease characterized by the degeneration of intervertebral discs (IVDs), accompanied by imbalance of metabolic and inflammatory homeostasis. Current studies establish that IVD degeneration is induced by increased apoptosis of nucleus pulposus (NP) cells. However, the underlying mechanisms of NP cell survival/apoptosis are not well elucidated. Here, we reveal a novel mechanism by which mTORC1 signaling controls NP cell survival through regulating metabolic homeostasis. We demonstrated that hyperactivated mTORC1 activity induced by inflammatory cytokines engenders the apoptosis of NP cells, whereas pharmacological inhibition of mTORC1 activity promotes NP cell survival. Using an integrative approach spanning metabolomics and biochemical approaches, we showed that mTORC1 activation enhanced glucose metabolism and lactic acid production, and therefore caused NP cell apoptosis. Our study identified mTORC1 in NP cells as a novel target for IVD degeneration, and provided potential strategies for clinical intervention of lumbar disc herniation.
DOI: 10.6084/m9.figshare.12695489.v1
title: Additional file 3 of Overexpression of TGFβ1 in murine mesenchymal stem cells improves lung inflammation by impacting the Th17/Treg balance in LPS-induced ARDS mice

description: Additional file 3.
DOI: 10.6084/m9.figshare.14788422.v1
title: Additional file 2 of Genetic differentiation and restricted gene flow in rice landraces from Yunnan, China: effects of isolation-by-distance and isolation-by-environment

description: Additional file 2: Table S1. List of samples included in the study, including their origin and group. Table S2. Summary of sequenced genes and primer sequences used in this study. Table S3. Summary of nucleotide polymorphisms and neutrality tests. Table S4. The genetic diversity of rice landraces based on 48 SSRs. Table S5. The genetic diversity of rice landraces in each population. Table S6. Putative selective-sweep regions in subgroup jap-N. Table S7. Putative selective-sweep regions in subgroup jap-S. Table S8. Putative selective-sweep regions in subgroup ind-N. Table S9. Putative selective-sweep regions in subgroup ind-S. Table S10. Cloned genes located on the selection-sweep regions. Table S11. QTLs that overlapped with selection-sweep regions. Table S12. SNPs with absolute value greater than 6 for the first three components of 24 environmental factors. Table S13. SNPs with the top 25 highest |z|-scores among those candidate genes with known functions. Table S14. Summary of SSR markers and primer sequences. Table S15. The detailed information of 24 environmental factors in each population. Table S16. Summary of agronomic traits for rice landraces in each population. The value before the slash shows the data collection from Yunnan, and the value after the slash shows the data collection from Hainan.
DOI: 10.6084/m9.figshare.14788359.v1
title: Additional file 1 of Geographic distribution of the cagA, vacA, iceA, oipA and dupA genes of Helicobacter pylori strains isolated in China

description: Additional file 1: The phylogenetic tree of cagA gene.