DOI: 10.5061/dryad.dn58p
title: Data from: Hierarchical structure of ecological and non-ecological processes of differentiation shaped ongoing gastropod radiation in the Malawi Basin

description: Ecological processes, non-ecological processes or a combination of both
 may cause reproductive isolation and speciation, but their specific roles
 and potentially complex interactions in evolutionary radiations remain
 poorly understood, which defines a central knowledge gap at the interface
 of microevolution and macroevolution. Here I examine genome scans in
 combination with phenotypic and environmental data to disentangle how
 ecological and non-ecological processes contributed to population
 differentiation and speciation in an ongoing radiation of Lanistes
 gastropods from the Malawi Basin. I found a remarkable hierarchical
 structure of differentiation mechanisms in space and time: neutral and
 mutation-order processes are older and occur mainly between regions,
 whereas more recent adaptive processes are the main driver of genetic
 differentiation and reproductive isolation within regions. The strongest
 differentiation occurs between habitats and between regions, i.e. when
 ecological and non-ecological processes act synergistically. The
 structured occurrence of these processes based on the specific geographic
 setting and ecological opportunities strongly influenced the potential for
 evolutionary radiation. The results highlight the importance of
 interactions between various mechanisms of differentiation in evolutionary
 radiations, and suggest that non-ecological processes are important in
 adaptive radiations, including those of cichlids. Insight into such
 interactions is critical to understanding large-scale patterns of
 organismal diversity.
description: Environmental data at
 sampling localities[primary data file] This
 file contains information on five environmental variables documented at
 the studied sample localitiesLanistesEnvData.txtResults of BayeScan[output file] This file contains the results of the BayeScan analysis. This output file has been used to identify loci that are putatively under selection.BayeScan_Output_fst.txtRaw amplified fragment length polymorphism dataset (NA = 9)[primary data file] This file contains the raw AFLP dataset with NA’s coded as '9'.gen_morph_pop_nine.txtRaw amplified fragment length polymorphism dataset (NA = ?)[primary data file] This file contains the raw AFLP dataset with NA’s coded as ‘?’.gen_morph_pop_questionmark.txtInformation on genetic diversity[output file] This file contains results from analysis of the AFLP dataset with AFLPsurv. Some of the tests reported in the paper have been performed on this output file.AFLPsurv-geneticdiversity.txtPopulation pairwise Fst data[output file] This file contains results from analysis of the AFLP dataset with AFLPsurv. These results have been used as input for the Fst-Pst comparisons.AFLPsurv-PopPairwiseFST.txt1000 Bootstrap replicates on Population pairwise Fsts[output file] This file contains results from analysis of the AFLP dataset with AFLPsurv. These results have been used to calculate 95 % confidence intervals on the Population pairwise Fst data.LanistesAFLPsurv_pairwiseFSTreplicates.txtGeometric morphometric dataset[primary data file] This file contains information on individual specimens and the coordinates from semilandmark morphometrics after Procrustes superimposition and semilandmark alignment with perpendicular projection. This dataset has been used as the dataset for all subsequent morphometric analyses including ordination and the calculation of population pairwise Psts.LanistesAFLP-MorphometricCoordsCS.txtPst summary statistics (from 1000 bootstraps)[output file] These summary statistics have been calculated from 1000 bootstrap replicates of the geometric morphometric dataset and subsequent Pst calculations. These summary statistics are used for Fst-Pst comparisons.PST_1000BootstrapReplicates.txtFst summary statistics (from 1000 bootstraps)[output file] These summary statistics have been calculated from 1000 bootstrap replicates of AFLPsurv. These summary statistics are used for Fst-Pst comparisons.FST_95CIfromBootstrappingAFLPsurv.txtData for Fst-Pst comparisons[input file] This file contains the compiled data from Pst and Fst calculations in an organized format to do Fst-Pst comparisons.PstFst-comparisondataFinal.txtInput for analyses with SPAGeDi (Basin-wide)[input file] This file is the file used to do basin-wide analyses with SPAGeDi.Lanistes_spagedi_Final.txtInput for analyses with SPAGeDi (Northern region)[input file] This file is the file used to do SPAGeDi-analyses on populations from the northern region.Lan_north_spagedi_Final.txtInput for analyses with SPAGeDi (Southern region)[input file] This file is the file used to do SPAGeDi-analyses on populations from the southern region.Lan_south_spagedi_Final.txtSPAGeDI output summary for Fi = 0.0[output file] This file summarizes the results of basin-wide and regional analyses with SPAGeDi under an inbreeding coefficient Fi = 0.0 (thus under Hardy-Weinberg equilibrium).kinship-distance_matrix_HW_Fi0_0.txtSPAGeDI output summary for Fi = 0.2[output file] This file summarizes the results of basin-wide and regional analyses with SPAGeDi under an inbreeding coefficient Fi = 0.2.kinship-distance_matrix_Fi0_2.txtSPAGeDI output summary for Fi = 0.4[output file] This file summarizes the results of basin-wide and regional analyses with SPAGeDi under an inbreeding coefficient Fi = 0.4.kinship-distance_matrix_Fi0_4.txtSPAGeDI output summary for Fi = 0.6[output file] This file summarizes the results of basin-wide and regional analyses with SPAGeDi under an inbreeding coefficient Fi = 0.6.kinship-distance_matrix_Fi0_6.txt