DOI: 10.22002/stm7f-92898
title: Sulfolobus cells synchronized for division

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2011-01-15</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 1.5 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 6 sec, sponge for humidity</p>
  mdj2011-01-15-14_slicer8700.jpg: pili .  Sulfolobus acidocaldarius mdj2011-01-15-14_slicer8887.jpg: ESCRT .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-15-14/rawdata/</p>11jan15b_Bx8g4_016.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
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        11jan15b_Bx8g4_016_full.rec,  Reconstruction (Pixel Size 2.5 nm), 671.1 MB  
        
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        11jan15b_Bx8g4_016_full_median.rec,  Reconstruction (Pixel Size 2.5 nm), 5.4 GB  
        
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        11jan15b_Bx8g4_016.rec,  Reconstruction (Pixel Size 2.5 nm), 146.4 MB  
        
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        11jan15b_Bx8g4_016median.rec,  Reconstruction (Pixel Size 2.5 nm), 1.2 GB  
        
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        11jan15b_Bx8g4_016mode1.rec,  Reconstruction (Pixel Size 2.5 nm), 292.7 MB  
        
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