DOI: 10.5061/dryad.pc866t1w3
title: Dynamic metabolic and molecular changes during seasonal shrinking in <em>Sorex araneus</em>

description: To meet the challenge of wintering in place many high-latitude small
 mammals reduce energy demands through hibernation. In contrast,
 short-lived Eurasian common shrews,Sorex araneus, remain active and
 shrink, including energy-intensive organs in winter, regrowing in spring
 in an evolved strategy called Dehnel’s phenomenon. How this size change is
 linked to metabolic and regulatory changes to sustain their high
 metabolism is unknown. We analyzed metabolic, proteomic, and gene
 expression profiles spanning the entirety of Dehnel’s seasonal cycle in
 wild shrews. We show regulatory changes to oxidative phosphorylation and
 increased fatty acid metabolism during autumn-to-winter shrinkage, as
 previously found in hibernating species. But in shrews we also found
 upregulated winter expression of genes involved in gluconeogenesis: the
 biosynthesis of glucose from non-carbohydrate substrates. Co-expression
 models revealed changes in size and metabolic gene expression interconnect
 via FOXO signaling, whose overexpression reduces size and extends lifespan
 in many model organisms. We propose that while shifts in gluconeogenesis
 meet the challenge posed by high metabolic rate and active winter
 lifestyle, FOXO signaling is central to Dehnel’s phenomenon, with spring
 downregulation limiting lifespan in these shrews.
description: # Dynamic metabolic and molecular changes during seasonal shrinking in
 *Sorex araneus*
 [https://doi.org/10.5061/dryad.pc866t1w3](https://doi.org/10.5061/dryad.pc866t1w3) Here we present the supplemental data for the analyses conducted in the above manuscript. File information is discussed within the manuscript and below. ## Change Log From the previous version, this data set now includes raw proteomic data, metabolomic data, full transcriptomic results, and a new WGCNA analyses that correlates network change to body size rather than liver size. Additionally, we have removed the code to reproduce these analyses, which can now be found on our GitHub to more accurately track changes https://github.com/wrthomas315/Dehnels_Seasonal_RNAseq2 .  ## Description of the data and file structure Supplementary data consists of 10 tables described below. **Supplemental Data Information** **Supplemental Data 1** – Sample information including (IDs, body mass, season, organ masses) and sequencing information (RIN, reads, filtering, mapping percentage). **Supplemental Data 2** – Metabolomics data. Data includes 250 metabolites tested (one-way ANOVA) for differential concentrations between seasons (raw and normalized). 250 metabolites tested required at least 2 levels of identification. Additionally, raw data for each level (1, 2a, 2b, 3; See Materials and Methods for descriptions) of identification included. **Supplemental Data 3** – Metabolite (n=250) one-way ANOVA test for significant differential concentrations (p&lt;0.05) between seasons. **Supplemental Data 4** – Likelihood Ratio Test results, gene clustering, and pathway enrichment of gene expression clusters. **Supplemental Data 5** – DESeq2 results and pathway enrichment for differential gene expression of the liver comparisons between summer juveniles and winter juveniles. **Supplemental Data 6** – Differential abundance, pathway enrichment, and raw data for summer juveniles versus winter juvenile proteomic comparisons. **Supplemental Data 7** – Hibernation pathway enrichment and overlap of significant genes with Dehnel’s phenomenon. **Supplemental Data 8** – DESeq2 results and pathway enrichment for differential gene expression of the liver comparisons between winter juveniles and spring adults. **Supplemental Data 9** – Differential abundance, pathway enrichment, and raw data for winter juveniles versus spring adult proteomic comparisons. **Supplemental Data 10** – List of WGCNA modules memberships and correlations with body size.