cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1626721
title: Cytotoxicity and intracellular dissolution of nickel nanowires

description: The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis, and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage, and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 μm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.
DOI: 10.6084/m9.figshare.1626721.v1
title: Cytotoxicity and intracellular dissolution of nickel nanowires

description: The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis, and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage, and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 μm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.
DOI: 10.6084/m9.figshare.1626686
title: Specific detection of Type 1 and Type 2 isolates of <i>Pyrenochaeta lycopersici</i> by loop-mediated isothermal amplification reaction

description: <i>Pyrenochaeta lycopersici</i> is the causal agent of corky root, an important soilborne disease of tomato (<i>Lycopersicon esculentum</i>) and other solanaceous crops. <i>P. lycopersici</i> isolates are classified into Types 1 and 2 on the basis of several physiological and molecular features. In this study, we aimed to establish a loop-mediated isothermal amplification (LAMP) method to identify Types 1 and 2 isolates. Using specific LAMP primer sets for Types 1 and 2, both types were easily identified within 35 min. The LAMP method demonstrated equal sensitivity to the polymerase chain reaction in molecular identification of the pathogen in cultured mycelia, infested plant roots, and their surrounding soil. The LAMP technology will contribute to the detection and identification of the pathogen, crop protection, and thorough understanding about the ecology of corky root disease of tomato.
DOI: 10.6084/m9.figshare.1626686.v1
title: Specific detection of Type 1 and Type 2 isolates of <i>Pyrenochaeta lycopersici</i> by loop-mediated isothermal amplification reaction

description: <i>Pyrenochaeta lycopersici</i> is the causal agent of corky root, an important soilborne disease of tomato (<i>Lycopersicon esculentum</i>) and other solanaceous crops. <i>P. lycopersici</i> isolates are classified into Types 1 and 2 on the basis of several physiological and molecular features. In this study, we aimed to establish a loop-mediated isothermal amplification (LAMP) method to identify Types 1 and 2 isolates. Using specific LAMP primer sets for Types 1 and 2, both types were easily identified within 35 min. The LAMP method demonstrated equal sensitivity to the polymerase chain reaction in molecular identification of the pathogen in cultured mycelia, infested plant roots, and their surrounding soil. The LAMP technology will contribute to the detection and identification of the pathogen, crop protection, and thorough understanding about the ecology of corky root disease of tomato.
DOI: 10.6084/m9.figshare.1626667
title: Systematic revision and molecular phylogeny of the land snail genus <i>Fruticocampylaea</i> (Gastropoda: Hygromiidae) from the Caucasus region

description: This paper presents a systematic revision and a molecular phylogenetic analysis of the Caucasian land snail genus <i>Fruticocampylaea</i>. The genus is newly delimited based on the reduction of the cavities adjoining the seminal duct in the penial papilla. Shell and genitalia of all five species (<i>F. narzanensis</i>, <i>F. kobensis</i>, <i>F. tushetica</i> sp. nov., <i>F. christophori</i>, <i>F. daghestana</i>) are described and figures provided. All synonyms and all locality records are listed. Maximum likelihood and Bayesian analyses of mitochondrial and nuclear DNA sequences (fragments of <i>cox1</i>, 16S rDNA, ITS2 and 28S rDNA) confirm the monophyly of <i>Fruticocampylaea</i>. The reduction of the dart apparatus and the conical plug, via which the dart apparatus inserts into the vagina, as well as the molecular phylogenetic analyses, suggests a sister group relationship between <i>Fruticocampylaea</i> and <i>Circassina</i> (without <i>Abchasohela</i>). Furthermore, the molecular phylogenetic analyses indicate that the <i>Fruticocampylaea</i> species originated in a rapid radiation. The uplift of the Greater Caucasus in the Late Miocene or Pliocene or climatic changes at the end of the Pliocene or in the early Pleistocene may have caused the radiation of <i>Fruticocampylaea</i>. Low intraspecific variability can be explained by population bottlenecks during Pleistocene glacial periods followed by postglacial population increase. http://zoobank.org/urn:lsid:zoobank.org:pub:AB15158D-21A3-4945-8D49-F7DE8E406E2B
DOI: 10.6084/m9.figshare.1626667.v2
title: Systematic revision and molecular phylogeny of the land snail genus <i>Fruticocampylaea</i> (Gastropoda: Hygromiidae) from the Caucasus region

description: This paper presents a systematic revision and a molecular phylogenetic analysis of the Caucasian land snail genus <i>Fruticocampylaea</i>. The genus is newly delimited based on the reduction of the cavities adjoining the seminal duct in the penial papilla. Shell and genitalia of all five species (<i>F. narzanensis</i>, <i>F. kobensis</i>, <i>F. tushetica</i> sp. nov., <i>F. christophori</i>, <i>F. daghestana</i>) are described and figures provided. All synonyms and all locality records are listed. Maximum likelihood and Bayesian analyses of mitochondrial and nuclear DNA sequences (fragments of <i>cox1</i>, 16S rDNA, ITS2 and 28S rDNA) confirm the monophyly of <i>Fruticocampylaea</i>. The reduction of the dart apparatus and the conical plug, via which the dart apparatus inserts into the vagina, as well as the molecular phylogenetic analyses, suggests a sister group relationship between <i>Fruticocampylaea</i> and <i>Circassina</i> (without <i>Abchasohela</i>). Furthermore, the molecular phylogenetic analyses indicate that the <i>Fruticocampylaea</i> species originated in a rapid radiation. The uplift of the Greater Caucasus in the Late Miocene or Pliocene or climatic changes at the end of the Pliocene or in the early Pleistocene may have caused the radiation of <i>Fruticocampylaea</i>. Low intraspecific variability can be explained by population bottlenecks during Pleistocene glacial periods followed by postglacial population increase. http://zoobank.org/urn:lsid:zoobank.org:pub:AB15158D-21A3-4945-8D49-F7DE8E406E2B
DOI: 10.6084/m9.figshare.1626665
title: Oxygen isotope composition of North American bobcat (<i>Lynx rufus</i>) and puma (<i>Puma concolor</i>) bone phosphate: implications for provenance and climate reconstruction

description: Feline carnivores are threatened by illegal wildlife trade. Tracing the provenance of unknown felid tissues via stable isotope analysis could provide important information in wildlife crime investigations. The oxygen isotope composition of mammalian skeletal phosphate (δ<sup>18</sup>O<sub>p</sub>) is widely applied to trace the origin of animal remains and to reconstruct migratory patterns in palaeontological, archaeological, ecological and wildlife forensic applications. Teeth and bones of terrestrial mammals form at constant body temperature in isotope equilibrium with body water, which is predominantly controlled by ingested meteoric water (δ<sup>18</sup>O<sub>w</sub>) that varies systematically with latitude, altitude and climate. Here we analysed δ<sup>18</sup>O<sub>p</sub> of 106 North American puma and bobcat bones of known geographic origin to establish the first δ<sup>18</sup>O<sub>p</sub>−δ<sup>18</sup>O<sub>w</sub> regression for feline carnivores: δ<sup>18</sup>O<sub>p</sub> = 0.40(±0.04) * δ<sup>18</sup>O<sub>w</sub> + 20.10(±0.40) (<i>R</i><sup>2</sup> = 0.46, <i>n</i> = 106). This was compared with those from their respective prey species (deer and rabbit), a canid carnivore (fox) and other placental mammals. Effects of species, sex and relative humidity on the feline δ<sup>18</sup>O<sub>p</sub>−δ<sup>18</sup>O<sub>w</sub> correlation were analysed and additional intra-individual tissue comparisons (hair δ<sup>18</sup>O<sub>h</sub> vs. bone δ<sup>18</sup>O<sub>p</sub>) were performed for some bobcat individuals. Bobcats and pumas exhibited only a moderate δ<sup>18</sup>O<sub>p</sub>−δ<sup>18</sup>O<sub>w</sub> correlation, which differed from canid carnivores and other placental mammals. However, feline δ<sup>18</sup>O<sub>p</sub> values revealed a moderate relation with δ<sup>18</sup>O<sub>w</sub>, which lacks for the δ<sup>18</sup>O<sub>h</sub> of hair from the same bobcat individuals. This indicates a difference in oxygen isotope routing from body water to bioapatite and hair. Most herbivores and omnivores track δ<sup>18</sup>O<sub>w</sub> in their bioapatite δ<sup>18</sup>O<sub>p</sub> values much better, whereas δ<sup>18</sup>O<sub>p</sub> and especially δ<sup>18</sup>O<sub>h</sub> values of feline carnivores are less precise proxies for meteoric water δ<sup>18</sup>O<sub>w</sub> values and thus for provenance determination in wildlife forensics and palaeoclimate reconstructions. Oxygen isotope fingerprinting of bobcat and puma is biased by factors related to their diet, behaviour and metabolism that need to be better understood.
DOI: 10.6084/m9.figshare.1626666.v2
title: The resurrection of the genus <i>Bergia</i> (Anthozoa, Zoantharia, Parazoanthidae)

description: The genus <i>Bergia</i> was established by Duchassaing de Fonbressin and Michelotti in 1860, for two species, <i>Bergia catelunaris</i> and <i>B. vialactea</i>. Subsequently, in 1903 Duerden recognized these two species as conspecific, and used <i>B. catelunaris</i> in favour of <i>B. vialactea</i>, and transferred <i>B. catelunaris</i> to the genus <i>Parazoanthus</i>. However, over the last decade, it has been found that the genus <i>Parazoanthus</i> is actually polyphyletic and therefore it has gradually been divided and redefined. Based on phylogenetic analyses, <i>Parazoanthus sensu stricto</i> was recently limited to species which form associations with sponges, but it still comprised of three distinctive and monophyletic subclades. Of these clades, one <i>Parazoanthus</i> clade contains the type species for <i>Parazoanthus</i>, <i>P. axinellae</i>, while another clade was recently described as the genus <i>Umimayanthus</i> based on mitochondrial 16S-rDNA sequences. However, the other remaining <i>Parazoanthus</i> clade contains <i>P. catenularis</i>, the original type species of the genus <i>Bergia</i>. Based on a concatenated set of published data for five molecular markers (18S-rDNA, 28S-rDNA, ITS1/5.8S/ITS2-rDNA, 16S-rDNA and COI-DNA), this study confirms the monophyly of <i>Bergia</i> in the context of Parazoanthidae. The phylogenetic analyses strongly support the resurrection of the genus <i>Bergia</i> as a valid taxonomic unit.
DOI: 10.6084/m9.figshare.1626667.v1
title: Systematic revision and molecular phylogeny of the land snail genus <i>Fruticocampylaea</i> (Gastropoda: Hygromiidae) from the Caucasus region

description: This paper presents a systematic revision and a molecular phylogenetic analysis of the Caucasian land snail genus <i>Fruticocampylaea</i>. The genus is newly delimited based on the reduction of the cavities adjoining the seminal duct in the penial papilla. Shell and genitalia of all five species (<i>F. narzanensis</i>, <i>F. kobensis</i>, <i>F. tushetica</i> sp. nov., <i>F. christophori</i>, <i>F. daghestana</i>) are described and figures provided. All synonyms and all locality records are listed. Maximum likelihood and Bayesian analyses of mitochondrial and nuclear DNA sequences (fragments of <i>cox1</i>, 16S rDNA, ITS2 and 28S rDNA) confirm the monophyly of <i>Fruticocampylaea</i>. The reduction of the dart apparatus and the conical plug, via which the dart apparatus inserts into the vagina, as well as the molecular phylogenetic analyses, suggests a sister group relationship between <i>Fruticocampylaea</i> and <i>Circassina</i> (without <i>Abchasohela</i>). Furthermore, the molecular phylogenetic analyses indicate that the <i>Fruticocampylaea</i> species originated in a rapid radiation. The uplift of the Greater Caucasus in the Late Miocene or Pliocene or climatic changes at the end of the Pliocene or in the early Pleistocene may have caused the radiation of <i>Fruticocampylaea</i>. Low intraspecific variability can be explained by population bottlenecks during Pleistocene glacial periods followed by postglacial population increase. http://zoobank.org/urn:lsid:zoobank.org:pub:AB15158D-21A3-4945-8D49-F7DE8E406E2B
DOI: 10.6084/m9.figshare.1626665.v1
title: Oxygen isotope composition of North American bobcat (<i>Lynx rufus</i>) and puma (<i>Puma concolor</i>) bone phosphate: implications for provenance and climate reconstruction

description: Feline carnivores are threatened by illegal wildlife trade. Tracing the provenance of unknown felid tissues via stable isotope analysis could provide important information in wildlife crime investigations. The oxygen isotope composition of mammalian skeletal phosphate (δ<sup>18</sup>O<sub>p</sub>) is widely applied to trace the origin of animal remains and to reconstruct migratory patterns in palaeontological, archaeological, ecological and wildlife forensic applications. Teeth and bones of terrestrial mammals form at constant body temperature in isotope equilibrium with body water, which is predominantly controlled by ingested meteoric water (δ<sup>18</sup>O<sub>w</sub>) that varies systematically with latitude, altitude and climate. Here we analysed δ<sup>18</sup>O<sub>p</sub> of 106 North American puma and bobcat bones of known geographic origin to establish the first δ<sup>18</sup>O<sub>p</sub>−δ<sup>18</sup>O<sub>w</sub> regression for feline carnivores: δ<sup>18</sup>O<sub>p</sub> = 0.40(±0.04) * δ<sup>18</sup>O<sub>w</sub> + 20.10(±0.40) (<i>R</i><sup>2</sup> = 0.46, <i>n</i> = 106). This was compared with those from their respective prey species (deer and rabbit), a canid carnivore (fox) and other placental mammals. Effects of species, sex and relative humidity on the feline δ<sup>18</sup>O<sub>p</sub>−δ<sup>18</sup>O<sub>w</sub> correlation were analysed and additional intra-individual tissue comparisons (hair δ<sup>18</sup>O<sub>h</sub> vs. bone δ<sup>18</sup>O<sub>p</sub>) were performed for some bobcat individuals. Bobcats and pumas exhibited only a moderate δ<sup>18</sup>O<sub>p</sub>−δ<sup>18</sup>O<sub>w</sub> correlation, which differed from canid carnivores and other placental mammals. However, feline δ<sup>18</sup>O<sub>p</sub> values revealed a moderate relation with δ<sup>18</sup>O<sub>w</sub>, which lacks for the δ<sup>18</sup>O<sub>h</sub> of hair from the same bobcat individuals. This indicates a difference in oxygen isotope routing from body water to bioapatite and hair. Most herbivores and omnivores track δ<sup>18</sup>O<sub>w</sub> in their bioapatite δ<sup>18</sup>O<sub>p</sub> values much better, whereas δ<sup>18</sup>O<sub>p</sub> and especially δ<sup>18</sup>O<sub>h</sub> values of feline carnivores are less precise proxies for meteoric water δ<sup>18</sup>O<sub>w</sub> values and thus for provenance determination in wildlife forensics and palaeoclimate reconstructions. Oxygen isotope fingerprinting of bobcat and puma is biased by factors related to their diet, behaviour and metabolism that need to be better understood.
DOI: 10.6084/m9.figshare.1626666
title: The resurrection of the genus <i>Bergia</i> (Anthozoa, Zoantharia, Parazoanthidae)

description: The genus <i>Bergia</i> was established by Duchassaing de Fonbressin and Michelotti in 1860, for two species, <i>Bergia catelunaris</i> and <i>B. vialactea</i>. Subsequently, in 1903 Duerden recognized these two species as conspecific, and used <i>B. catelunaris</i> in favour of <i>B. vialactea</i>, and transferred <i>B. catelunaris</i> to the genus <i>Parazoanthus</i>. However, over the last decade, it has been found that the genus <i>Parazoanthus</i> is actually polyphyletic and therefore it has gradually been divided and redefined. Based on phylogenetic analyses, <i>Parazoanthus sensu stricto</i> was recently limited to species which form associations with sponges, but it still comprised of three distinctive and monophyletic subclades. Of these clades, one <i>Parazoanthus</i> clade contains the type species for <i>Parazoanthus</i>, <i>P. axinellae</i>, while another clade was recently described as the genus <i>Umimayanthus</i> based on mitochondrial 16S-rDNA sequences. However, the other remaining <i>Parazoanthus</i> clade contains <i>P. catenularis</i>, the original type species of the genus <i>Bergia</i>. Based on a concatenated set of published data for five molecular markers (18S-rDNA, 28S-rDNA, ITS1/5.8S/ITS2-rDNA, 16S-rDNA and COI-DNA), this study confirms the monophyly of <i>Bergia</i> in the context of Parazoanthidae. The phylogenetic analyses strongly support the resurrection of the genus <i>Bergia</i> as a valid taxonomic unit.
DOI: 10.6084/m9.figshare.1626666.v1
title: The resurrection of the genus <i>Bergia</i> (Anthozoa, Zoantharia, Parazoanthidae)

description: The genus <i>Bergia</i> was established by Duchassaing de Fonbressin and Michelotti in 1860, for two species, <i>Bergia catelunaris</i> and <i>B. vialactea</i>. Subsequently, in 1903 Duerden recognized these two species as conspecific, and used <i>B. catelunaris</i> in favour of <i>B. vialactea</i>, and transferred <i>B. catelunaris</i> to the genus <i>Parazoanthus</i>. However, over the last decade, it has been found that the genus <i>Parazoanthus</i> is actually polyphyletic and therefore it has gradually been divided and redefined. Based on phylogenetic analyses, <i>Parazoanthus sensu stricto</i> was recently limited to species which form associations with sponges, but it still comprised of three distinctive and monophyletic subclades. Of these clades, one <i>Parazoanthus</i> clade contains the type species for <i>Parazoanthus</i>, <i>P. axinellae</i>, while another clade was recently described as the genus <i>Umimayanthus</i> based on mitochondrial 16S-rDNA sequences. However, the other remaining <i>Parazoanthus</i> clade contains <i>P. catenularis</i>, the original type species of the genus <i>Bergia</i>. Based on a concatenated set of published data for five molecular markers (18S-rDNA, 28S-rDNA, ITS1/5.8S/ITS2-rDNA, 16S-rDNA and COI-DNA), this study confirms the monophyly of <i>Bergia</i> in the context of Parazoanthidae. The phylogenetic analyses strongly support the resurrection of the genus <i>Bergia</i> as a valid taxonomic unit.
DOI: 10.6084/m9.figshare.1626661
title: Health state utility values associated with advanced gastric, oesophageal, or gastro-oesophageal junction adenocarcinoma: a systematic review

description: Objectives:To systematically identify utility values associated with advanced gastric cancer (GC), oesophageal cancer (OC), or gastro-oesophageal junction (GEJ) cancer. Utility values relating to health states are an essential component for cost-utility analysis (CUA).Methods:MEDLINE, Embase, Cochrane Library, and EconLit databases were reviewed for relevant studies using a pre-defined search strategy. Studies eligible for inclusion reported health state utility values (HSUVs) using direct (standard gamble [SG] and time-trade-off [TTO]) and indirect (such as EuroQol 5D [EQ-5D], short-form 6D [SF-6D], and the 15-dimensional instrument [15D]) methods for patients with advanced GC, OC, or GEJ cancer.Results:A total of 539 unique publications were identified, of which eight met the inclusion criteria (GC, <i>n</i> = 2; mixed population [gastrointestinal cancers], <i>n</i> = 4; OC, <i>n</i> = 2). The most commonly used instrument to estimate HSUVs was the EQ-5D (<i>n</i> = 7). Utilities were also estimated using the SF-6D and the 15D in the same study (<i>n</i> = 1). Direct elicitation methods included the TTO (<i>n</i> = 2) and SG (<i>n</i> = 1). Across the eight identified publications<i>,</i> health states and study populations were heterogeneous and sample sizes were limited.Limitations:This review, as with all summaries of this nature, is only as robust as the data derived from the identified studies. The systematic review process does not resolve any design issues or biases associated with the original studies.Conclusions:Limited data estimate HSUVs in patients with advanced GC, OC<i>,</i> or GEJ cancer. Utilities for advanced GC alone and advanced OC alone were reported in only two publications for each cancer type. No publications considered advanced GEJ utilities alone, and four publications considered utilities for a mixed population of gastrointestinal cancer types. Comparisons are confounded by heterogeneity across the identified publications. Further research into HSUVs associated with advanced GC and OC is required to improve the evidence available for use in CUAs.