DOI: 10.22002/rhzxj-4tf49
title: Wild type NA1000

description: NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle.Keywords: FtsZ filaments.
description: <strong>Tilt Series Date:</strong> 2006-05-01</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, Amira</p>
<strong>Collaborators and Roles:</strong> Cells were cultured by Zhuo Li. Data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle.</p>
<strong>Sample Preparation:</strong> 4 ul of cell culture at different time points were mixed with 10nm colloidal gold and plunge-frozen onto glow-discharged Quantifoil R2/2, R2/1, or R1.2/1.3 grids using a Vitrobot (FEI).</p>
  wt75minpyeMay010634k180e12u061.jpg: low mag
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/rawdata/</p>wt75minpyeMay010634k180e12u06.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/rawdata/wt75minpyeMay010634k180e12u06.mrc"
        > <i class="download icon"></i> Download </a></p>

        wt75minpye06.rec.mrc,  Reconstruction (Pixel Size 1.34 nm), 202.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/3dimage_680/wt75minpye06.rec.mrc"
        > <i class="download icon"></i> Download </a></p>

        wt75minpye06_full.rec,  Reconstruction (Pixel Size 1.34 nm), 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/3dimage_681/wt75minpye06_full.rec"
        > <i class="download icon"></i> Download </a></p>

        stalk6FL.am,  Segmentation, 3.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/file_1301/stalk6FL.am"
        > <i class="download icon"></i> Download </a></p>

        stalk6FL.surf,  Segmentation, 832.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/file_1302/stalk6FL.surf"
        > <i class="download icon"></i> Download </a></p>

        stalk6IM.am,  Segmentation, 4.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/file_1303/stalk6IM.am"
        > <i class="download icon"></i> Download </a></p>

        stalk6IM.surf,  Segmentation, 3.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/file_1304/stalk6IM.surf"
        > <i class="download icon"></i> Download </a></p>

        stalk6OM.am,  Segmentation, 4.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/file_1305/stalk6OM.am"
        > <i class="download icon"></i> Download </a></p>

        stalk6OM.surf,  Segmentation, 3.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-05-01-6/file_1306/stalk6OM.surf"
        > <i class="download icon"></i> Download </a></p>