cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/hvcew-1kp79
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-310/rawdata/</p>JV9298_PD-1-3_236.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-310/rawdata/JV9298_PD-1-3_236.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_PD-1-3_236_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-310/3dimage_124281/JV9298_PD-1-3_236_SIRT_1k.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yg888-22779
title: Xanthomonas WT

description: Xac WT pBRaφ pBRR
description: <strong>Tilt Series Date:</strong> 2019-12-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Carvalho and Farah lab sent the strain. Debnath Ghosal collected data</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in 2xTY with apt antibiotics, freeze cells next day after about 14 hour when they are in stationary phase.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 5 sec. ** Use 7 sec for next sessions.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-6/rawdata/</p>Xac_No1_TS47.mrc,  Tilt Series (Pixel Size 0.353 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-6/rawdata/Xac_No1_TS47.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_No1_TS47_full.rec,  Reconstruction (Pixel Size 1.412 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-6/3dimage_112328/Xac_No1_TS47_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p0ct3-9x422
title: HeLa (Kyoto) - PM damaged

description: HeLa cell stably expresses CHMP4B-EGFP at low levels (courtesy MPI). grid - WY41_grid3.
description: <strong>Tilt Series Date:</strong> 2018-05-15</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, caused plasma membrane damage using Laser and froze. Shrawan performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-05-15-13/rawdata/</p>TS20.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-05-15-13/rawdata/TS20.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS20_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-05-15-13/3dimage_102307/TS20_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jrqkm-s0p23
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-255/3dimage_124179/</p>JV9298_PD-1-3_179_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-255/3dimage_124179/JV9298_PD-1-3_179_SIRT_1k.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_PD-1-3_179.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-255/rawdata/JV9298_PD-1-3_179.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g87fj-ps143
title: Legionella_JV9246

description: Legionella_JV9246: DotF-sfGFP fusion to the wild-type gene on the chromosome (thyA) and ΔdotO.
description: <strong>Tilt Series Date:</strong> 2019-05-28</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-36/rawdata/</p>JV9246_area1_tomo0018.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-36/rawdata/JV9246_area1_tomo0018.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9246_area1_tomo0018_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-36/3dimage_103447/JV9246_area1_tomo0018_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r4q46-g2m77
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-118/rawdata/</p>Pseudomonas_JT2944Tilt520.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-118/rawdata/Pseudomonas_JT2944Tilt520.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudomonas_JT2944Tilt520_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-118/3dimage_96285/Pseudomonas_JT2944Tilt520_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/73bzh-a6687
title: ec11-3_lam1_ts1

description: .  [added 2019-10-15 15:25:39] IT 0.4s
description: <strong>Tilt Series Date:</strong> 2019-05-27</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 1094</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: °, constant angular increment, dosage: 64.0 eV/Å²,  defocus: -4.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 3ml cultures of E. coli 1094 and cultivated 37C - 220rpm ON OD600 5.4 on the next day. Diluted 50x in Minimal Media After 3hours, turbid media became clear and flakes appeared. Cells were concentrated (10min - 3000rcf - 20C) at OD 20 (based on the OD of the cellulase culture that doesn't make flakes)</p>
<strong>Sample Preparation:</strong> 27C - 95% hum Manual backblotting 7s (no glare seen) DT1'' Grid was FIB milled</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-05-27-1/rawdata/</p>ec11-3_lam1_ts1_dose_weight.st,  Tilt Series (Pixel Size 0.45 nm), 5.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-05-27-1/rawdata/ec11-3_lam1_ts1_dose_weight.st"
        > <i class="download icon"></i> Download </a></p>

        ec11-3_lam1_ts1_dose_weight.rec,  Reconstruction (Pixel Size 1.8 nm), 308.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-05-27-1/3dimage_106879/ec11-3_lam1_ts1_dose_weight.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.4fqee7
title: Occurrence Download

description: A dataset containing 1346 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorbaria sorbifolia",
 "Country is Estonia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1346 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002528-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/adfy3-3x821
title: ff22-1-4_cell2

description: F22-1-4 ; 1.2s
description: <strong>Tilt Series Date:</strong> 2018-10-04</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter xylinus</p>
<strong>Strain:</strong> F2-2, Δcmcax</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 50.0°), step: 2°, constant angular increment, dosage: 121.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> F2-2 strain from Tomonori Nakai</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Shaken culture grown until OD600 of 1.1 and ten diluted to 0.55 (0.5X)</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 Cu NH2 Finder - Glowdischarged 15mA 1min - autoblot BT 4s - BF 0% 22deg - hum 100%</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-10-04-4/rawdata/</p>ff2-1-4_cell3.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-10-04-4/rawdata/ff2-1-4_cell3.mrc"
        > <i class="download icon"></i> Download </a></p>

        ff2-1-4_cell3_full.rec,  Reconstruction (Pixel Size 0.96 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-10-04-4/3dimage_97404/ff2-1-4_cell3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0asns-bjb82
title: MEF cell Drp1 Knockout

description: Raw data files of MEF cell Drp1 Knockout
description: <strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -2.5 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang grew and froze cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown on R2/2 quantifoil gold grids coated with 0.1 mg/ml fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, 20min 200nM mitotracker Red, plunge frozen and FIB milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-7/rawdata/</p>TS3.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-7/rawdata/TS3.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS3_bin4_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 862.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-7/3dimage_101822/TS3_bin4_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.eh4ha6
title: Occurrence Download

description: A dataset containing 1905 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorbaria sorbifolia",
 "Country is France",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1905 records from 31 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002530-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/s3x64-2gc79
title: bio12-2_lam2_ts3

description: .  [added 2019-10-11 16:53:00] IT 0.8s
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: °, constant angular increment, dosage: 88.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-6/rawdata/</p>bio12-2_lam2_ts3.st,  Tilt Series (Pixel Size 0.48 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-6/rawdata/bio12-2_lam2_ts3.st"
        > <i class="download icon"></i> Download </a></p>

        bio12-2_lam2_ts3.rec,  Reconstruction (Pixel Size 1.8 nm), 166.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-6/3dimage_106839/bio12-2_lam2_ts3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/76are-pxc40
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-89/3dimage_123863/</p>JV9298_PD-1-3_455_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-89/3dimage_123863/JV9298_PD-1-3_455_SIRT_1k.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_PD-1-3_455.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-89/rawdata/JV9298_PD-1-3_455.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2c63sp
title: Occurrence Download

description: A dataset containing 3748 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorbaria sorbifolia",
 "Country is Finland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 3748 records from 36 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002529-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/g0rsc-j1f45
title: bl21_1-1-4_sq1_cell2

description: BL21_1-1-4 - SQ1 - IT1s
description: <strong>Tilt Series Date:</strong> 2019-02-22</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK1, BL21 dHisdHA RQABpCDF+EFGpRSF S2-ZC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: °, constant angular increment, dosage: 87.0 eV/Å²,  defocus: -4.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 3mL culture started from glycerol stock. TB + salts + Kan (40ug/mL) + Strepto (70ug/mL) incubated at 37C - 220rpm until OD 1.5 (overdue a little bit). IPTG added and incubated at 17C-ON.OD at 3.4 before freezing the next day.</p>
<strong>Sample Preparation:</strong> Diluted to OD 0.4 - Broad 100% 22C - Manual backblotting 4s, glare seen at 2s</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-02-22-6/rawdata/</p>bl21_1-1-4_sq1_cell2_orig.st,  Tilt Series (Pixel Size 0.54 nm), 1.7 GB  
        
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        bl21_1-1-4_sq1_cell2.rec,  Reconstruction (Pixel Size 2.164 nm), 160.4 MB  
        
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