cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/b16hb-9z908
title: E.coli minicell/Pul T2SS

description: grid box YWC252strain PAP5359 (minCDE knockout)Cells were grown on plate before freezing
description: <strong>Tilt Series Date:</strong> 2017-05-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain provided by Ingrid Guilvout in Olivera Francetic lab</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-05-15-31/rawdata/</p>20170515_YWC252b_10034.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-05-15-31/rawdata/20170515_YWC252b_10034.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170515_YWC252b_10034_part121_5.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-05-15-31/3dimage_84573/20170515_YWC252b_10034_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hpb4qn
title: Occurrence Download

description: A dataset containing 105 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster horizontalis",
 "Country is Canada",
 "HasCoordinate is true"
 ]
}

 The dataset includes 105 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002159-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/m120h-05h88
title: twitching Pseudomonas WT

description: Box YWC219-1Cells incubated on grid to promote twitching
description: <strong>Tilt Series Date:</strong> 2016-08-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sample prepared in collaboration with Alex Persat</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-21-15/rawdata/</p>20160821_YWC219_20012.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-21-15/rawdata/20160821_YWC219_20012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160821_YWC219_20012_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-21-15/3dimage_78241/20160821_YWC219_20012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.xddad7
title: Occurrence Download

description: A dataset containing 2959 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cotoneaster horizontalis",
 "Country is Belgium",
 "HasCoordinate is true"
 ]
}

 The dataset includes 2959 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002157-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/2wtcz-26e04
title: B. subtilis AD3268

description: Alp7AR dynamic filaments in skinny Bacillus
description: <strong>Tilt Series Date:</strong> 2015-03-29</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> AD3268, ponA null, Alp7A</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joe Pogliano and Alan Derman</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-03-29-2/rawdata/</p>32680002.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-03-29-2/rawdata/32680002.mrc"
        > <i class="download icon"></i> Download </a></p>

        32680002_part121_22.rec,  Reconstruction (Pixel Size 2.0 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-03-29-2/3dimage_53441/32680002_part121_22.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y3pss-3kn32
title: T9_wt

description: This was a sample prepared when Paul visited
description: <strong>Tilt Series Date:</strong> 2017-02-05</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Paul Veith and Eric Reynolds</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-05-20/rawdata/</p>sq160008.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-05-20/rawdata/sq160008.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq160008_part121_1.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-05-20/3dimage_82025/sq160008_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7cjp4-3dj94
title: Saccharomyces cerevisiae - JAWY1

description: Raw data files of Saccharomyces cerevisiae - JAWY1
description: <strong>Tilt Series Date:</strong> 2016-10-31</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-10-31-4to5 (TS3,TS4)- Heat shocked @38 deg C for 2min10min. Other sm2016-10-31-* - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.</p>
<strong>Sample Preparation:</strong> Final OD of 0.2 on grids (in growth medium) and blotted from behind</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-3/3dimage_80815/</p>TS13_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-3/3dimage_80815/TS13_full.rec"
        > <i class="download icon"></i> Download </a></p>

        TS13.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-3/rawdata/TS13.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS13_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 200.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-3/3dimage_81450/TS13_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rgeeqr
title: Occurrence Download

description: A dataset containing 388760 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Themeda triandra, Thermopsis dolichocarpa, Thespesia lampas, Thespesia populnea, Thevetia peruviana, Thinopyrum elongatum, Thinopyrum intermedium, Thinopyrum pycnanthum, Thladiantha dubia, Thlaspi alliaceum, Thlaspi arvense, Thunbergia alata, Thunbergia fragrans, Thunbergia grandiflora, Thunbergia laurifolia, Thymelaea passerina, Thymophylla pentachaeta, Thymus praecox, Thymus vulgaris, Thyrsacanthus nitidus)",
 "BasisOfRecord is one of (Machine Observation, Human Observation, Observation)",
 "HasCoordinate is true",
 "Year is greater than or equal to 1990"
 ]
}

 The dataset includes 388760 records from 1490 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002133-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15602043
title: Idaho Temperature-Derived Metrics (Frost-Free Days & Growing Degree Days) sum (1980-2022)

description: Annual sum of frost free days (FFD) in the state of Idaho between 1980 and 2022.  These data were processed by ISU's GIS TREC with source data from the DAYMET dataset. https://daac.ornl.gov/DAYMET/guides/Daymet_Daily_V4R1.html

Note: The frost free days (daily Tmin > 0° C) do not need to be consecutive to be included in this analysis.

Annual sum of growing degree days (GDD) in the state of Idaho between 1980 and 2022.  These data were processed by ISU's GIS TREC with source data from the DAYMET dataset. https://daac.ornl.gov/DAYMET/guides/Daymet_Daily_V4R1.html

Note: GDD was calculated using a base temperature of 0° C following the methodology described here https://www.canr.msu.edu/uploads/files/Research_Center/NW_Mich_Hort/General/CalculatingGrowingDegreeDays.pdf
DOI: 10.5281/zenodo.15602044
title: Idaho Temperature-Derived Metrics (Frost-Free Days & Growing Degree Days) sum (1980-2022)

description: Annual sum of frost free days (FFD) in the state of Idaho between 1980 and 2022.  These data were processed by ISU's GIS TREC with source data from the DAYMET dataset. https://daac.ornl.gov/DAYMET/guides/Daymet_Daily_V4R1.html

Note: The frost free days (daily Tmin > 0° C) do not need to be consecutive to be included in this analysis.

Annual sum of growing degree days (GDD) in the state of Idaho between 1980 and 2022.  These data were processed by ISU's GIS TREC with source data from the DAYMET dataset. https://daac.ornl.gov/DAYMET/guides/Daymet_Daily_V4R1.html

Note: GDD was calculated using a base temperature of 0° C following the methodology described here https://www.canr.msu.edu/uploads/files/Research_Center/NW_Mich_Hort/General/CalculatingGrowingDegreeDays.pdf
DOI: 10.22002/5ptt3-mrp85
title: JV9082_K3_janelia

description: JV9082:DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2019-12-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -2.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-54/rawdata/</p>9082_Box1_No1_S154.mrc,  Tilt Series (Pixel Size 0.261 nm), 1.7 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        9082_Box1_No1_S154_part37_4.rec,  Reconstruction (Pixel Size 1.044 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-54/3dimage_112578/9082_Box1_No1_S154_part37_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a8etj-5g109
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-352/rawdata/</p>JV9082_20180901a0126.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-352/rawdata/JV9082_20180901a0126.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0126_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-352/3dimage_97058/JV9082_20180901a0126_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xqrh2-c0g30
title: Exp phase S. acidocaldarius

description: Grid box 82 - grid1. To study division belt.
description: <strong>Tilt Series Date:</strong> 2018-01-25</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for alignment and tomo3D for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were harvested at 0.06 OD and kept at 4 C for further steps until freezing. Spun cells at 6000 xg for 3 min to concentrated cells to ~4 OD and mixed with equal volume of 10 nm Au beads to reach final concentration of ~2 OD.</p>
<strong>Sample Preparation:</strong> Cu finder grids - glow discharged. Added 3.5 µL of suspension and blotted for 2-3 sec from behind manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-25-2/rawdata/</p>TS2_full.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-25-2/rawdata/TS2_full.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS2_4bin_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-25-2/3dimage_102333/TS2_4bin_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mj560-kj688
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-217/rawdata/</p>JV9082_201808290217.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-217/rawdata/JV9082_201808290217.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_201808290217_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-217/3dimage_96828/JV9082_201808290217_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wdng0-x6x32
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-362/rawdata/</p>Hpylori_DFliF_FISE_367.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_367_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>