DOI: 10.5061/dryad.76hdr7t80
title: Data from: Isoflurane anesthesia alters <sup>31</sup>P magnetic resonance spectroscopy markers compared to awake mouse brain

description: Phosphorus magnetic resonance spectroscopy (³¹P MRS) enables noninvasive
 measurement of brain energy metabolism. Preclinical studies are typically
 performed under isoflurane anesthesia, which can alter neuronal activity
 and metabolism. To address this, we compared ³¹P MRS in awake,
 MR-habituated mice and under isoflurane anesthesia. We demonstrate that
 awake ³¹P MRS in MR-habituated mice is feasible and provides spectra of
 comparable quality to those acquired under anesthesia. Importantly, we
 show that isoflurane alters brain metabolism, reducing PCr, intracellular
 pH, and PME levels.
description: # Data from: Isoflurane anesthesia alters ^31^P magnetic resonance
 spectroscopy markers compared to awake mouse brain Dataset DOI:
 [10.5061/dryad.76hdr7t80](https://doi.org/10.5061/dryad.76hdr7t80) ##
 Description of the data and file structure These data were collected to
 investigate brain energy metabolism in mice using in vivo phosphorus
 magnetic resonance spectroscopy (³¹P MRS). Experiments included
 inter-variability, intra-variability, and awake versus anesthetized
 conditions. For each experiment, respiration rate and body temperature
 were recorded during ISIS scans, ³¹P MR spectra were acquired and
 processed in TopSpin, and metabolite integrals were extracted. **Note on
 temperature measurements:** In the awake versus anesthetized experiments,
 temperature was measured on the abdominal surface, which underestimates
 core body temperature by ~3.5 °C. For mice M1 and F2, the temperature
 probe was slightly misplaced; however, body temperature was maintained in
 the same fashion as in all other experiments, and there is no reason to
 believe that actual body temperature deviated from 37 °C. Full
 experimental and processing details are described in the Methods section
 of the associated publication:\ Saba Molhemi, Rasmus West Knopper,
 Christian Stald Skoven, Thomas Beck Lindhardt, Caroline Degel, Leif
 Østergaard, Brian Hansen*, 2025. "Isoflurane anesthesia alters 31P
 magnetic resonance spectroscopy markers compared to awake mouse
 brain". PLOS One. DOI: *10.1371/journal.pone.0333627 ### Files and
 variables #### File: Dryad.zip **Description:** This dataset contains the
 minimal data underlying the analyses reported in the associated
 manuscript.\ Data are organized by experiment: * Inter-variability *
 Intra-variability * Awake vs. Anesthetized Each experiment folder includes
 three subfolders: **1. AUC** **Folder:** `AUC/`\ Contains `.csv` files
 with extracted area-under-the-curve (AUC) values of ³¹P metabolites. *
 **File naming convention:** `[MouseID][Condition]and/or[Scan]_AUC.csv` *
 *MouseID* = animal identifier (e.g., M1, F4). M = male, F = female. *
 *Condition* = awake or anes (for anesthetized).\ Each file corresponds to
 one individual mouse under one condition. * Scan = ISIS scan number. *
 **File contents:** 1. `Object` — name of the metabolite whose AUC was
 extracted; the rows appear in the following sequence: PME, Pi, PDE, PCr,
 γ-ATP, α-ATP, β-ATP. 2. `Integral [abs]` — absolute area-under-the-curve
 (AUC) value for the metabolite (arbitrary units). 3. `Integral [rel]` —
 relative AUC normalized to the phosphocreatine (PCr) signal. 4. `Peak
 [ppm]` — chemical shift position of the metabolite peak (in parts per
 million, ppm). - **Rows:**\ Each row in `Object` lists one ³¹P metabolite
 from the spectrum of a single mouse, with its respective `Integral [abs]`,
 `Integral [rel]`, and `Peak [ppm]` values in the next columns.  **2.
 Biometry** **Folder:** `Biometry/`\ Contains `.txt` files recording
 respiration rate and body temperature during each ISIS scan. * **File
 naming convention:** `[MouseID]_[Condition]_biometry.txt` * *MouseID* =
 animal identifier (e.g., M1, F4). * *Condition* = `awake` or `anes`
 or `ISIS_scan_number`\ Each file corresponds to the raw biometry log for
 one mouse in one condition. * **File contents:** * **Column headers:** 1.
 `Time` — time elapsed since the start of the recording (in seconds). 2.
 `Resp Rate` — respiration rate at each time point (in respirations per
 minute). 3. `Temp 1` — body temperature (in °C). - **Rows:**\ Each row
 represents one time point recorded during the ISIS scan, providing the
 instantaneous respiration rate and temperature of the mouse.  **3.
 Spectra** **Folder:** `Spectra/`\ Contains a folder with the naming
 convention  `[MouseID][Condition]and/or[Scan]`  with one
 `ascii-spec.txt` file in each folder with the preprocessed ³¹P MR spectra
 exported from TopSpin. * **File contents:** * The file has four
 comma-separated columns: 1. **Index** – a sequential data point number
 (integer) used for reference. 2. **Intensity** – the MRS signal amplitude
 in arbitrary units (a.u.), corresponding to the y-axis. 3. **Frequency** –
 the raw spectrometer frequency value in Hz (optional for most analyses).
 4. **Chemical Shift** – the chemical shift in parts per million (ppm),
 corresponding to the x-axis. * **Rows:**\ Each row corresponds to one
 spectral point. The spectrum is ordered from higher to lower ppm. For
 plotting, use the chemical shift (Column 4) as the x-axis and the
 intensity (Column 2) as the y-axis.