cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8194847.v1
title: Stability and phosphorus leaching of tilapia feed in water

description: ABSTRACT:  The present research aimed to investigate the stabil¬¬ity of pellets and phosphorus leaching of diets formulated for juveniles of Nile tilapia (Oreochromis niloticus), with different sources of phosphorus and different exposure times in water. Six diets were elaborated by varying the source of phosphorus (1 ‒ dicalcium phosphate (DP); 2 ‒ meat and bone meal (MBM); 3 ‒ poultry meal (PM); 4 ‒ anchovy meal (AM); 5 ‒ tilapia filleting industrial meal (TM); 6 ‒ calcined bone meal (CBM)) and, then, were submitted to four exposure times in water (5, 10, 15 and 20 minutes), with three replicates. Thus, 72 aquariums of 30‒liters were used, each being an experimental unit. All diets were evaluated for electrical conductivity of water, turgidity of pellets, mineral matter leaching, flotation of pellets, and total phosphorus leaching. Only turgidity and flotation of pellets varied with the different sources of phosphorus in the diets. The MBM diet had the highest turgidity of pellets. The PM, AM, and CBM diets had the highest flotation of pellets. The total phosphorus leaching had a linear effect with the increase of the exposure time, showing a greater release of phosphorus in the water with increase of exposure time. Data showed that PM, AM, and CBM diets had less potential impact on the aquatic environment. Conversely, the TM diet has greater polluting potential. These results showed that diets formulated with different sources of phosphorus exhibit distinct actions in the water, providing different effects on the fish culture environment.
DOI: 10.6084/m9.figshare.8194850
title: Changes in ascorbic acid and total phenolics contents associated with browning inhibition of pineapple slices

description: Abstract Pineapple slices were treated with three antioxidants and stored under passive modified atmosphere packaging (MAP) to evaluate the effect of these treatments on tissue browning (percentage of dark area) and the accumulation of ascorbic acid and total phenolics. Pineapple slices were immersed in ascorbic acid (AA), isoascorbic acid (IAA), and N-acetylcysteine (NAC) solutions for 2 min, and then were packed and stored at 5 °C under air (AIR) or MAP for 15 days. Water was used as control. The application of IAA and NAC under passive MAP reduced better the tissue browning index and maintained a good quality of the slices. The use of AA and IAA increased the contents of ascorbic acid and total phenolics. The application of IAA followed by storage under MAP conditions was effective in reducing tissue browning, which was associated with a higher retention of ascorbic acid and total phenolics contents of the pineapple slices.
DOI: 10.6084/m9.figshare.8194859.v1
title: Concentrated beta-galactosidase and cell permeabilization from Saccharomyces fragilis IZ 275 for beta-galactosidase activity in the hydrolysis of lactose

description: Abstract The cheese whey shows an organic nutrient charge that can be used to obtain metabolites of interest by biotechnology of microorganisms. Thus, fermentative processes for enzyme production, in particular beta-galactosidase becomes feasible. The enzyme plays an important role in the biotech food industry to obtain milk and dairy products with low lactose content for consumption by intolerant individuals. The objective of this work was to determine the enzyme activity of the concentrated beta-galactosidase (CBG) and the permeabilized cells (PC) both obtained from Saccharomyces fragilis IZ 275. The enzyme beta-galactosidase obtained from the fermentation of Saccharomyces fragilis IZ 275 in cheese whey was used to determine the optimal conditions for the hydrolysis of lactose solution at 1% (w/v). Response Surface Methodology (RSM) by Box-Behnken Design (BBD) was employed to determine beta-galactosidase activity for such factors pH, temperature and enzyme concentration suitable for the lactose hydrolysis. Based on the statistical analysis, the optimum operational conditions for maximizing lactose hydrolysis thus optimizing the enzyme activity for CBG were, temperature 30 °C, pH 6.0 and enzyme concentration 3% (v/v) and for PC was temperature 44 °C, pH 7.0 and enzyme concentration 4% (v/v).
DOI: 10.6084/m9.figshare.8194865.v1
title: Streamlined Synthesis of 6-((1H-1,2,3-Triazol-4-yl)methyl)-1H-pyrrolo [3,4-d]pyridazin-1-one System via Sequential N-Alkylation, CuAAC, and [4 + 2] Cyclization Reactions

description: An efficient sequential three-step reaction methodology for the synthesis of three new series-1-(prop-2-yn-1-yl)-1H-pyrroles, methyl 4-acetyl-1-((1H-1,2,3-triazol-4-yl)methyl)-1H-pyrrole-3-carboxylates and 6-((1H-1,2,3-triazol-4-yl)methyl)-2,6-dihydro-1H-pyrrolo[3,4-d]pyridazin-1-ones-is reported. The methodology comprises: (i) N-alkylation reactions of polyfunctionalized 1H-pyrroles-which were previously obtained from (E)-methyl 2-azido-3-arylacrylates-with propargyl bromide in order to obtain 1H-pyrroles; (ii) standard copper-catalyzed azide-alkyne cycloaddition (CuAAC) involving organic azides (benzyl-, 4-methoxybenzyl- and 4-chlorobenzyl, as well as n-octyl azide) and N-propargylated 1H-pyrroles to give triazolyl derivatives, as methyl 1H-pyrrole-3-carboxylates (click chemistry); and (iii) [4 + 2] cyclocondensation reactions of the ketoesters in the presence of hydrazine hydrochloride in order to furnish the desired series of pyrrolo[3,4-d]pyridazin-1-ones at total yields up to 54%.
DOI: 10.6084/m9.figshare.8194868
title: IYPT 2019 - INTERNATIONAL YEAR OF THE PERIODIC TABLE OF THE CHEMICAL ELEMENTS

description: United Nations elected 2019 a year devoted to the Periodic Table of the Elements, in deference to the original contribution by Dmitri Ivanovich Mendeleev in 1869. Since then, the impact of the Periodic Table in Science has been tremendous, becoming an icon today as the Chemistry Portal, leading to a better acquaintance of the chemical elements. In this article, modern aspects of the Periodic Table of the Elements are presented, complementing an existing historical presentation in this Journal, with special emphasis on the quantum distribution models and on the remarkable role of the elements in Science and Nanotechnology.
DOI: 10.6084/m9.figshare.8194874.v1
title: ENANTIOSELECTIVE ORGANOCATALYSIS: EVOLUTION AND RECENT ASPECTS

description: In the last two decades, enantioselective organocatalysis has established itself as one of the three pillars of asymmetric catalysis. The rapid growth in the area is due to the rationalization of organocatalysis based on the generic modes of catalyst activation, being applied to several types of reactions, in a rather generic and predictable way and providing high enantioselectivities. This tutorial review presents the evolution of this area through a brief discussion on all generic modes of activation previously systematized in the literature: activation via enamine, iminium ion, hydrogen-bonding, counterion, SOMO, photoredox, carbene and phase-transfer, and the recent advances in the area.
DOI: 10.6084/m9.figshare.8194883.v1
title: Effect of convective and microwave methods on drying characteristics, color, rehydration and microstructure properties of ginger

description: Abstract In this research, the effects of convective drying method (60, 70 and 80 °C) and microwave drying method (160 and 350 W) on the drying characteristics, color, rehydration and microstructure properties of ginger has been examined. For the purpose of choosing the most appropriate thin-layer drying models in terms of the drying applications, nine mathematical models have been applied on the experimental data. Pursuant to the assessment of the statistical tests, models of Diffusion Approach, Midilli et al. and Page that represent drying characteristics are found to be better than other models. Drying applications have affected the color values of all fresh ginger samples. Results indicated that browning degree increases as drying temperature and microwave power rise. The samples dried at 60 °C has the maximum rehydration ratio while the samples dried at 80 °C has the minimum rehydration ratio. Scanning electron microscopy visuals have ascertained that the deterioration of the ginger samples remarkably surged due to the rise in either drying temperature or microwave power. Applying the convective and microwave techniques as an alternative drying method for ginger samples is possible.
DOI: 10.6084/m9.figshare.8194886
title: Treatment Options and Outcomes of Penile Constriction Devices

description: ABSTRACT  Purpose: To study the effect of penile constriction devices used on a large series of patients who presented at our emergency facility. We explored treatment options to prevent a wide range of vascular and mechanical injuries occurring due to penile entrapment.  Materials and Methods: Between January 2001 and March 2016, 26 patients with penile entrapment were admitted to our facility and prospectively evaluated.  Results: The time that elapsed from penile constrictor application to hospital admission varied from 10 hours to 6 weeks (mean: 22.8 hours). Non-metallic devices were used by 18 patients (66.6%) while the other nine (33.4%) had used metallic objects. Acute urinary retention was present in six (23%) patients, of whom four (66.6%) underwent percutaneous surgical cystotomy and two (33.4%) underwent simple bladder catheterization. The main reason for penile constrictor placement was erectile dysfunction, accounting for 15 (55.5%) cases. Autoerotic intention, psychiatric disorders, and sexual violence were responsible in five (18.5%), five (18.5%), and two (7.4%) cases, respectively. The mean hospital stay was 18 hours (range, 6 hours to 3 weeks).  Conclusion: Penile strangulation treatment must be immediate through the extraction of the foreign body, avoiding vascular impairments that can lead to serious complications. Most patients present with low-grade injuries and use penile constrictors due to erectile dysfunction. Removal of constrictor device can be challenging. The use of specific tools for achieving penile release from constrictors is a fast, safe and effective method. Patients with urinary retention may require urinary diversion.
DOI: 10.6084/m9.figshare.8194886.v1
title: Treatment Options and Outcomes of Penile Constriction Devices

description: ABSTRACT  Purpose: To study the effect of penile constriction devices used on a large series of patients who presented at our emergency facility. We explored treatment options to prevent a wide range of vascular and mechanical injuries occurring due to penile entrapment.  Materials and Methods: Between January 2001 and March 2016, 26 patients with penile entrapment were admitted to our facility and prospectively evaluated.  Results: The time that elapsed from penile constrictor application to hospital admission varied from 10 hours to 6 weeks (mean: 22.8 hours). Non-metallic devices were used by 18 patients (66.6%) while the other nine (33.4%) had used metallic objects. Acute urinary retention was present in six (23%) patients, of whom four (66.6%) underwent percutaneous surgical cystotomy and two (33.4%) underwent simple bladder catheterization. The main reason for penile constrictor placement was erectile dysfunction, accounting for 15 (55.5%) cases. Autoerotic intention, psychiatric disorders, and sexual violence were responsible in five (18.5%), five (18.5%), and two (7.4%) cases, respectively. The mean hospital stay was 18 hours (range, 6 hours to 3 weeks).  Conclusion: Penile strangulation treatment must be immediate through the extraction of the foreign body, avoiding vascular impairments that can lead to serious complications. Most patients present with low-grade injuries and use penile constrictors due to erectile dysfunction. Removal of constrictor device can be challenging. The use of specific tools for achieving penile release from constrictors is a fast, safe and effective method. Patients with urinary retention may require urinary diversion.
DOI: 10.6084/m9.figshare.8194889
title: Determination of L-Ascorbic Acid in Milk by Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry Analysis

description: This study provides a fast, accurate and reproducible method for L-ascorbic acid (L-AA) determination in milk samples by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). A small volume of a low toxicity organic solvent (ethanol) was used for degreasing and deproteinization steps. Ethylenediaminetetraacetic acid disodium salt (EDTA) and formic acid were used as stabilizing agents. The method was successfully validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and inter/intra-day precision and applied in raw and processed milk samples. Good linearity (r2 &gt; 0.9915) and low LOD and LOQ, 1.5 and 5.0 µg L-1, respectively, were obtained. The recoveries for 500 and 1000 µg L-1 spikes were higher than 90% and the precision values expressed in terms of relative standard deviation (RSD) were ≤ 6.8%. For the first time, the determination of L-AA in a 500 µg L-1 concentration range was reported for milk samples.
DOI: 10.6084/m9.figshare.8194889.v1
title: Determination of L-Ascorbic Acid in Milk by Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry Analysis

description: This study provides a fast, accurate and reproducible method for L-ascorbic acid (L-AA) determination in milk samples by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). A small volume of a low toxicity organic solvent (ethanol) was used for degreasing and deproteinization steps. Ethylenediaminetetraacetic acid disodium salt (EDTA) and formic acid were used as stabilizing agents. The method was successfully validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and inter/intra-day precision and applied in raw and processed milk samples. Good linearity (r2 &gt; 0.9915) and low LOD and LOQ, 1.5 and 5.0 µg L-1, respectively, were obtained. The recoveries for 500 and 1000 µg L-1 spikes were higher than 90% and the precision values expressed in terms of relative standard deviation (RSD) were ≤ 6.8%. For the first time, the determination of L-AA in a 500 µg L-1 concentration range was reported for milk samples.
DOI: 10.6084/m9.figshare.8194895
title: Cytomorphological analysis of cervical cytological smears of women aged over 60 years

description: ABSTRACT  Introduction: Cervicovaginal atrophy is a condition that can affect women after menopause, and cytology is a diagnostic tool useful in such cases.  Objective: To evaluate the cytomorphological profile of cervical smears in patients over 60 years old.  Methods: Cytopathological examinations of 500 patients over 60 years old were selected consecutively in this cross-sectional, quantitative, retrospective study.  Results: Only 114 (22.8%) presented the squamocolumnar junction (SCJ) sampled, and their presence decreased progressively with advancing age (p &lt; 0.001). Most smears (95.6%) were classified as atrophic. Microbiological analysis showed that from the 22 non-atrophic smears, most presented lactobacillus flora. Among the atrophic swabs, the predominant flora was cocci, with 47.2%. Only 4% presented cytological changes: atypical squamous cells of undetermined significance [(ASC-US) - eight cases/40%], atypical squamous cells - cannot exclude high-grade squamous intraepithelial lesion [(ASC-H) - five cases/25%], high-grade squamous intraepithelial lesion [(HSIL) - three/15%], low-grade squamous intraepithelial lesion [(LSIL) - two cases/10%] and adenocarcinoma in situ [(ACI) - two cases/10%]. Among the modified smears, four (20%) presented SCJ cells, and four patients (20%) took hormones (from these, two cases of ASC-H (10%) and two cases ASC-US (10%), showing a relationship between the onset of the lesion and the use of hormones (p &lt; 0.05).  Conclusion: The absence of SCJ indicates a diagnostic limitation of sample collection. Although the frequency of lesions has been similar to other studies, and the recommended age range for the examination is between 25 and 60 years, it is important to note that many women older than this range should perform the collection of oncology cytology due to existence of elderly women with risk profile for the disease.
DOI: 10.6084/m9.figshare.8194895.v1
title: Cytomorphological analysis of cervical cytological smears of women aged over 60 years

description: ABSTRACT  Introduction: Cervicovaginal atrophy is a condition that can affect women after menopause, and cytology is a diagnostic tool useful in such cases.  Objective: To evaluate the cytomorphological profile of cervical smears in patients over 60 years old.  Methods: Cytopathological examinations of 500 patients over 60 years old were selected consecutively in this cross-sectional, quantitative, retrospective study.  Results: Only 114 (22.8%) presented the squamocolumnar junction (SCJ) sampled, and their presence decreased progressively with advancing age (p &lt; 0.001). Most smears (95.6%) were classified as atrophic. Microbiological analysis showed that from the 22 non-atrophic smears, most presented lactobacillus flora. Among the atrophic swabs, the predominant flora was cocci, with 47.2%. Only 4% presented cytological changes: atypical squamous cells of undetermined significance [(ASC-US) - eight cases/40%], atypical squamous cells - cannot exclude high-grade squamous intraepithelial lesion [(ASC-H) - five cases/25%], high-grade squamous intraepithelial lesion [(HSIL) - three/15%], low-grade squamous intraepithelial lesion [(LSIL) - two cases/10%] and adenocarcinoma in situ [(ACI) - two cases/10%]. Among the modified smears, four (20%) presented SCJ cells, and four patients (20%) took hormones (from these, two cases of ASC-H (10%) and two cases ASC-US (10%), showing a relationship between the onset of the lesion and the use of hormones (p &lt; 0.05).  Conclusion: The absence of SCJ indicates a diagnostic limitation of sample collection. Although the frequency of lesions has been similar to other studies, and the recommended age range for the examination is between 25 and 60 years, it is important to note that many women older than this range should perform the collection of oncology cytology due to existence of elderly women with risk profile for the disease.
DOI: 10.6084/m9.figshare.8194898
title: Polymorphisms in the IL17A gene are not involved in the development of preeclampsia in the Brazilian population

description: ABSTRACT  Introduction: Preeclampsia is defined by the development of hypertension associated with proteinuria after the 20th week of gestation in previously normotensive women. IL17A is a potent inducer of tissue inflammation and polymorphisms in the IL17A gene can modulate gene expression and affect the functioning of Th17 cells, strengthening susceptibility to preeclampsia.  Objective: To investigate the polymorphisms rs4711998 A&gt;G, rs8193036 C&gt;T and rs2275913 A&gt;G in the IL17A gene in women with preeclampsia.  Methods: This is a control case study, composed of 263 women, 89 with preeclampsia and 174 of the control group. The polymorphisms investigated by real time polymerase chain reaction (PCR) allele discrimination technique. The risk of IL17A polymorphisms contributing to preeclampsia was assessed by the inheritance model through logistic regression. Statistical power presented 99.5% for association detection. Statistical significance was defined as p &lt; 0.05.  Results: Genotype frequencies as well as multiple logistic regression analysis were not statistically significant for the rs4711998 A&gt;G, rs8193036 C&gt;T and rs2275913 A&gt;G polymorphisms of the IL17A gene. No association was found between any haplotypes of the polymorphisms investigated and the risk of developing PE.  Conclusion: There is no association between the allele frequencies, genotype, inheritance models and haplotypes of the rs4711998 A&gt;G, rs8193036 C&gt;T and rs2275913 A&gt;G polymorphisms of the IL17A gene and PE.
DOI: 10.6084/m9.figshare.8194898.v1
title: Polymorphisms in the IL17A gene are not involved in the development of preeclampsia in the Brazilian population

description: ABSTRACT  Introduction: Preeclampsia is defined by the development of hypertension associated with proteinuria after the 20th week of gestation in previously normotensive women. IL17A is a potent inducer of tissue inflammation and polymorphisms in the IL17A gene can modulate gene expression and affect the functioning of Th17 cells, strengthening susceptibility to preeclampsia.  Objective: To investigate the polymorphisms rs4711998 A&gt;G, rs8193036 C&gt;T and rs2275913 A&gt;G in the IL17A gene in women with preeclampsia.  Methods: This is a control case study, composed of 263 women, 89 with preeclampsia and 174 of the control group. The polymorphisms investigated by real time polymerase chain reaction (PCR) allele discrimination technique. The risk of IL17A polymorphisms contributing to preeclampsia was assessed by the inheritance model through logistic regression. Statistical power presented 99.5% for association detection. Statistical significance was defined as p &lt; 0.05.  Results: Genotype frequencies as well as multiple logistic regression analysis were not statistically significant for the rs4711998 A&gt;G, rs8193036 C&gt;T and rs2275913 A&gt;G polymorphisms of the IL17A gene. No association was found between any haplotypes of the polymorphisms investigated and the risk of developing PE.  Conclusion: There is no association between the allele frequencies, genotype, inheritance models and haplotypes of the rs4711998 A&gt;G, rs8193036 C&gt;T and rs2275913 A&gt;G polymorphisms of the IL17A gene and PE.
DOI: 10.6084/m9.figshare.8194901
title: Effect of acetylsalicylic acid on total myenteric neurons in mice experimentally infected with Trypanosoma cruzi

description: Abstract: We investigated the effects of acetylsalicylic acid (ASA) on the total myenteric neuronal population in the descending colon in Trypanosoma cruzi-infected mice. Thirty-five male Swiss mice, 60 days old, were divided into a control group (C group), control group treated with ASA (CA group), infected group (I group), and infected group treated with ASA (IA group). A total of 1300 trypomastigotes of the Y strain of T. cruzi were intraperitoneally inoculated in the IA and I groups. The CA and IA groups were treated with ASA intraperitoneally. At 75 days post-infection (dpi), all of the animals were sacrificed. Neurons in the colon were stained with Giemsa, quantified, and measured. No difference in the course of infection was observed between the IA and I groups, reflected by the parasitemia curve. Acetylsalicylic acid treatment in the CA and IA groups did not alter the total number of myenteric neurons compared with the C and I groups. The CA and IA groups exhibited an increase in the nuclear area, cytoplasmic area, and neuronal body area compared with the C and I groups. Future studies should elucidate the mechanism of action of ASA against Chagas’ disease in the chronic phase.