cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.1594/pangaea.909481
title: Seawater carbonate chemistry and metabolic rate, ventilation rate and critical oxygen partial pressure of Dosidicus gigas and Doryteuthis pealeii

description: Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood–oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (Pcrit), the PO2 below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO2-induced reduction in oxygen supply should be apparent as an increase in Pcrit. In this study, we assessed the effects of CO2 (46–143 Pa; 455–1410 μatm) on the metabolic rate and Pcrit of two squid species - Dosidicus gigas and Doryteuthis pealeii – through manipulative experiments. We also developed a model, with inputs for hemocyanin pH sensitivity, blood PCO2 and buffering capacity, that simulates blood oxygen supply under varying seawater CO2 partial pressures. We compare model outputs with measured Pcrit in squid. Using blood–O2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid–base regulation, an increase in seawater PCO2 to 100 Pa (1000 μatm) would result in a maximum drop in arterial hemocyanin–O2 saturation by 1.6% at normoxia and a Pcrit increase of 0.5 kPa. Our live-animal experiments support this supposition, as CO2 had no effect on measured metabolic rate or Pcrit in either squid species.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-11-20.
description: Supplement to: Birk, Matthew A; McLean, Erin L; Seibel, Brad A (2018): Ocean acidification does not limit squid metabolism via blood oxygen supply. Journal of Experimental Biology, 221(19), jeb187443
DOI: 10.1594/pangaea.868432
title: The effect of ocean acidification on marine soundscapes

description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-15.
DOI: 10.1594/pangaea.762350
title: Seawater carbonate chemistry and biological processes during experiments with Coccolithus braarudii, 2011

description: Ocean acidification and associated shifts in carbonate chemistry speciation induced by increasing levels of atmospheric carbon dioxide (CO2) have the potential to impact marine biota in various ways. The process of biogenic calcification, for instance, is usually shown to be negatively affected. In coccolithophores, an important group of pelagic calcifiers, changes in cellular calcification rates in response to changing ocean carbonate chemistry appear to differ among species. By applying a wider CO2 range we show that a species previously reported insensitive to seawater acidification, Coccolithusbraarudii, responds both in terms of calcification and photosynthesis, although at higher levels of CO2. Thus, observed differences between species seem to be related to individual sensitivities while the underlying mechanisms could be the same. On this basis we develop a conceptual model of coccolithophorid calcification and photosynthesis in response to CO2-induced changes in seawater carbonate chemistry speciation.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
description: Supplement to: Krug, Sebastian; Schulz, Kai Georg; Riebesell, Ulf (2011): Effects of changes in carbonate chemistry speciation on Coccolithus braarudii: a discussion of coccolithophorid sensitivities. Biogeosciences, 8(3), 771-777
DOI: 10.1594/pangaea.835120
title: The photo-physiological response of a model cnidarian-dinoflagellate symbiosis to CO2-induced acidification at the cellular level

description: We measured the relationship between CO2-induced seawater acidification, photo-physiological performance and intracellular pH (pHi) in a model cnidarian-dinoflagellate symbiosis - the sea anemone Aiptasia sp. -under ambient (289.94 ± 12.54 µatm), intermediate (687.40 ± 25.10 µatm) and high (1459.92 ± 65.51 µatm) CO2 conditions. These treatments represented current CO2 levels, in addition to CO2 stabilisation scenarios IV and VI provided by the Intergovernmental Panel on Climate Change (IPCC). Anemones were exposed to each treatment for two months and sampled at regular intervals. At each time-point we measured a series of physiological responses: maximum dark-adapted fluorescent yield of PSII (Fv/Fm), gross photosynthetic rate, respiration rate, symbiont population density, and light-adapted pHi of both the dinoflagellate symbiont and isolated host anemone cell. We observed increases in all but one photo-physiological parameter (Pgross:R ratio). At the cellular level, increases in light-adapted symbiont pHi were observed under both intermediate and high CO2 treatments, relative to control conditions (pHi 7.35 and 7.46 versus pHi 7.25, respectively). The response of light-adapted host pHi was more complex, however, with no change observed under the intermediate CO2 treatment, but a 0.3 pH-unit increase under the high CO2 treatment (pHi 7.19 and 7.48, respectively). This difference is likely a result of a disproportionate increase in photosynthesis relative to respiration at the higher CO2 concentration. Our results suggest that, rather than causing cellular acidosis, the addition of CO2 will enhance photosynthetic performance, enabling both the symbiont and host cell to withstand predicted ocean acidification scenarios.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-08-21.
description: Supplement to: Gibbin, Emma M; Davy, Simon K (2014): The photo-physiological response of a model cnidarian–dinoflagellate symbiosis to CO2-induced acidification at the cellular level. Journal of Experimental Marine Biology and Ecology, 457, 1-7
DOI: 10.1594/pangaea.836051
title: (Table 5) Seawater chemistry at Long Bay reef site, U.S. Virgin Islands
DOI: 10.1594/pangaea.836005
title: Land based mesocosm experiment studying the simultaneous impact of warming and acidification on the planktonic food web of the Eastern Mediterranean

description: A land based mesocosm experiment focusing on the study of the simultaneous impact of warming and acidification on the planktonic food web of the Eastern Mediterranean took place in August-September 2013 at the mesocosm facilities of HCMR in Crete (CRETACOSMOS). Two different pCO2 (present day and predicted for year 2100) were applied in triplicate mesocosms of 3 m**3. This was tested in two different temperatures (ambient seawater T and ambient T plus 3°C). Twelve mesocosms in total were incubated in two large concrete tanks. Temperature was controlled by sophisticated, automated systems. A large variety of chemical, biological and biochemical variables were studied, including salinity, temperature, light and alkalinity measurements, inorganic and organic, particulate and dissolved, nutrient analyses, biological stock (Chla concentration, enumeration and community composition of microbial, phyto- and zooplankton organisms) and rate (primary, bacterial, viral production, copepod egg production, zooplankton grazing, N2 fixation, P uptake) measurements, bacterial DNA extraction and phytoplankton transcriptomics, calcifiers analyses. Twenty three scientists from 6 Institutes and 5 countries participated in this experiment.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010).
DOI: 10.1594/pangaea.889425
title: Raw multibeam EM122 data: transits of SONNE cruise SO261 (South Pacific)

description: Latitude/Longitude extracted from DSHIP. Data acquisition was performed using a multibeam sonar system Simrad EM-122 from Kongsberg. Raw data are delivered within Simrad current multibeam vendor format. SIMRAD raw data can be processed i.e. with the software packages CARIS HIPS/SIPS, Caraibes, QPS Qimera or with the open source software package MB-System (http://www.ldeo.columbia.edu/res/pi/MB-System/).These data should not be used for navigational purposes.
DOI: 10.1594/pangaea.899865
title: Seawater carbonate chemistry and oxygen consumption, metabolite concentration

description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-03-25.
DOI: 10.1594/pangaea.922819
title: Seawater carbonate chemistry and reproduction, energy metabolism and fatty acid composition of Ophryotrocha japonica and Ophryotrocha robusta

description: Trans-generational plasticity (TGP) represents a primary mechanism for guaranteeing species persistence under rapid global changes. To date, no study on TGP responses of marine organisms to global change scenarios in the ocean has been conducted on phylogenetically closely related species, and we thus lack a true appreciation for TGP inter-species variation. Consequently, we examined the tolerance and TGP of life-history and physiological traits in two annelid species within the genus Ophryotrocha: one rare (O. robusta) and one common (O. japonica). Both species were exposed over two generations to ocean acidification (OA) and warming (OW) in isolation and in combination (OAW). Warming scenarios led to a decrease in energy production together with an increase in energy requirements, which was lethal for O. robusta before viable offspring could be produced by the F1. Under OA conditions, O. robusta was able to reach the second generation, despite showing lower survival and reproductive performance when compared to control conditions. This was accompanied by a marked increase in fecundity and egg volume in F2 females, suggesting high capacity for TGP under OA. In contrast, O. japonica thrived under all scenarios across both generations, maintaining its fitness levels via adjusting its metabolomic profile. Overall, the two species investigated show a great deal of difference in their ability to tolerate and respond via TGP to future global changes. We emphasize the potential implications this can have for the determination of extinction risk, and consequently, the conservation of phylogenetically closely related species.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.
DOI: 10.1594/pangaea.925182
title: Seawater carbonate chemistry and larval settlement of Sea urchin larvae

description: Extensive research has shown that the early life stages of marine organisms are sensitive to ocean acidification (OA). Less is known, however, on whether larval settlement and metamorphosis may be affected, or by which mechanisms. These are key processes in the life cycle of most marine benthic organisms, since they mark the transition between the free swimming larval stage to the benthic life. We investigated whether OA could affect the larval settlement success of the sea urchin Evechinus chloroticus, a key coastal species with ecological, economic and cultural importance in New Zealand. We performed four settlement experiments to test whether reduced seawater pH (ranging from 8.1 to 7.0, at an interval of ∼0.2 pH units) alters larval settlement and metamorphosis success. Our results show that settlement success was not significantly reduced when the larvae were exposed to a range of reduced seawater pH treatments (8.1–7.0) at time of settlement (direct effects). Similarly, when presented with crustose coralline algae (CCA) pre-conditioned in different seawater pH of either pH 8.1 or 7.7 for 28 days, larval settlement success remained unaltered (indirect effects). We conclude that competent larvae in this species are resilient to OA at time of settlement. Further research on a range of taxa that vary in settlement selectivity and behaviour is needed in order to fully understand the effects of OA on the life cycle of marine invertebrates and the consequences it might have for future coastal marine ecosystems.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-11-20.
DOI: 10.1594/pangaea.925329
title: Seawater carbonate chemistry and composition of plastic biofilm assemblages

description: The increasing quantity of plastic waste in the ocean is providing a growing and more widespread novel habitat for microbes. Plastics have taxonomically distinct microbial communities (termed the 'Plastisphere') and can raft these unique communities over great distances. In order to understand the Plastisphere properly it will be important to work out how major ocean changes (such as warming, acidification and deoxygenation) are shaping microbial communities on waste plastics in marine environments. Here, we show that common plastic drinking bottles rapidly become colonized by novel biofilm-forming bacterial communities, and that ocean acidification greatly influences the composition of plastic biofilm assemblages. We highlight the potential implications of this community shift in a coastal community exposed to enriched CO2 conditions.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-11-30.
DOI: 10.1594/pangaea.925450
title: Seawater carbonate chemistry and copepod adult size, egg production, and egg size and growth

description: Climate change is expected to exacerbate upwelling intensity and natural acidification in Eastern Boundaries Upwelling Systems (EBUS). Conducted between January-September 2015 in a nearshore site of the northern Humboldt Current System directly exposed to year-round upwelling episodes, this study was aimed at assessing the relationship between upwelling mediated pH-changes and functional traits of the numerically dominant planktonic copepod-grazer Acartia tonsa (Copepoda). Environmental temperature, salinity, oxygen, pH, alkalinity, chlorophyll-a (Chl), copepod adult size, egg production (EP), and egg size and growth were assessed through 28 random oceanographic surveys. Agglomerative clustering and multidimensional scaling identified three main di-similitude nodes within temporal variability of abiotic and biotic variables: A) “upwelling”, B) “non-upwelling”, and C) “warm-acid” conditions. Nodes A and B represented typical features within the upwelling phenology, characterized by the transition from low temperature, oxygen, pH and Chl during upwelling to higher levels during non-upwelling conditions. However, well-oxygenated, saline and “warm-acid” node C seemed to be atypical for local climatology, suggesting the occurrence of a low frequency oceanographic perturbation. Multivariate (LDA and ANCOVA) analyses revealed upwelling through temperature, oxygen and pH were the main factors affecting variations in adult size and EP, and highlighted growth rates were significantly lower under node C. Likely buffering upwelling pH-reductions, phytoplankton biomass maintained copepod reproduction despite prevailing low temperature, oxygen and pH levels in the upwelling setting. Helping to better explain why this species is among the most recurrent ones in these variable yet productive upwelling areas, current findings also provide opportune cues on plankton responses under warm-acid conditions, which are expected to occur in productive EBUS as a consequence of climate perturbations.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-12-08.
DOI: 10.1594/pangaea.925337
title: Seawater carbonate chemistry and copepod traits and performance

description: Linking pH/pCO2 natural variation to phenotypic traits and performance of foundational species provides essential information for assessing and predicting the impact of ocean acidification (OA) on marine ecosystems. Yet, evidence of such linkage for copepods, the most abundant metazoans in the oceans, remains scarce, particularly for naturally corrosive Eastern Boundary Upwelling systems (EBUs). This study assessed the relationship between pH levels and traits (body and egg size) and performance (ingestion rate (IR) and egg reproduction rate (EPR)) of the numerically dominant neritic copepod Acartia tonsa, in a year-round upwelling system of the northern (23° S) Humboldt EBUs. The study revealed decreases in chlorophyll (Chl) ingestion rate, egg production rate and egg size with decreasing pH as well as egg production efficiency, but the opposite for copepod body size. Further, ingestion rate increased hyperbolically with Chl, and saturated at 1 µg Chl/ L. Food resources categorized as high (H, &gt;1 µg/L) and low (L,  7.89) and future (&gt;400 µatm pCO2, pH &lt; 7.89) were used to compare our observations to values globally employed to experimentally test copepod sensitivity to OA. A comparison (PERMANOVA) test with Chl/pH (2*2) design showed that partially overlapping OA levels expected for the year 2100 in other ocean regions, low-pH conditions in this system negatively impacted traits and performance associated with copepod fitness. However, interacting antagonistically with pH, food resource (Chl) maintained copepod production in spite of low pH levels. Thus, the deleterious effects of ocean acidification are modulated by resource availability in this system.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-11-30.
DOI: 10.1594/pangaea.925454
title: Seawater carbonate chemistry and copepod reproduction

description: The combined upwelling-El Niño (EN) event regulation of the numerically dominant Acartia tonsa (Crustacea, Copepoda) reproduction was examined in a year-round upwelling system (23°S) of the Humboldt Eastern Boundary Upwelling System (EBUS) during the EN 2015. A previous analysis of the environmental regulation of this system is extended here by considering complementary oceanographic information (sea level, stratification indexes) and additional reproductive traits, such as maximum (MaxEPR), median (MedianEPR) and prevalence of egg producing females over a period of six months. Furthermore, field minimum-maximum pH levels were reproduced in three 96-h incubation experiments conducted under variable salinity conditions to evaluate copepod mean EPR, egg size and hatching success. Supporting previous assertions, the warm-high salinity EN 2015 was observed in the study site separately from hydrographic conditions associated with upwelling to non-upwelling regimes. Analysis of similarity-distance (Distance based Linear Model (DistLM)) and normalized data (separate-slope comparison under a General Linear Model (GLM)) showed that reproductive traits were regulated by specific combinations of ambient conditions, and that this regulation was also sensitive to the prevailing hydrographic regime. Thus, upwelling to non-upwelling transitions changing the pH, and EN-associated salinity and stratification shifts, were significantly and strongly linked to almost all reproductive traits (DistLM). Slope comparison (GLM) indicated MaxEPR and MedianEPR variations also underlie the phenology, highlighting the relationship between pH and salinity with biological variations. In conjunction with experimental observations, the current study consistently suggests that pH-variations in the upwelling realm, and EN hydrographic perturbations might underpin responses of plankton populations to climate change in productive EBUS.
description: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-12-08.