DOI: 10.5061/dryad.b8gtht77s
title: Data from: Optimization and characterization of PLGA nanoparticles loaded with Astaxanthin and evaluation of anti-photodamage effect in vitro

description: Astaxanthin is a xanthophyll carotenoid with high beneficial biological
 activities, such as antioxidant function and scavenging oxygen free
 radicals, but its application is limited because of poor water solubility
 and low bioavailability. Here, we prepared and optimized poly
 (lactic-co-glycolic acid) (PLGA) nanoparticles loaded with astaxanthin
 using the emulsion solvent evaporation technique and investigated the
 anti-photodamage effect in HaCaT cells. The four-factor three-stage
 Box-Behnken design was used to optimize the nanoparticle formulation. The
 experimental determination of the optimal nanoparticle size was 154.4 ±
 0.35 nm, the zeta potential was 22.07 ± 0.93 mV, encapsulation efficiency
 was 96.42 ± 0.73%, and drug loading capacity was 7.19 ± 0.12%. The
 physicochemical properties of the optimized nanoparticles were
 characterized by dynamic light scattering, SEM, TEM, FTIR, XRD, DSC, and
 TGA. In vitro study exhibited the excellent cell viability and cellular
 uptake of optimized nanoparticle on HaCaT cells. The anti-photodamage
 studies (cytotoxicity assay, ROS content, and JC-1 assessment)
 demonstrated that the optimized nanoparticles were more effective and
 safer than pure astaxanthin in HaCaT cells. These results suggest that our
 PLGA-coated astaxanthin nanoparticles synthesis method was highly
 feasible, and can be used in cosmetics or the treatment of skin diseases.
description: The Box–Behnken  design optimized
 the parameters and obtained the optimal process conditions of AST-PLGA
 NP The various physicochemical and
 morphological properties of the optimized AST-PLGA NP were
 characterized:Particle Size and zeta potential;Evaluation of the encapsulation
 efficiency and drug loading
 capacity ;Morphologic analysis of
 nanoparticles;Fourier
 Transform Infrared (FTIR) Spectroscopic;X-ray Diffraction Patterns
 (XRD);Thermal analysis. In
 <i>vitro</i> cell viability and cytotoxicity use MTT
 method. The antioxidant activity use ROS
 and JC-1 method.          
  
description: dataset: F.B.H-Table1.csv<br> Table1. Factors and levels
 used in the Box–Behnken design.<br> <br> dataset:
 F.B.H-Table2.csv<br> Table 2. Box-Behnken Design Matrix and Observed
 Response Value <br> dataset:
 F.B.H-Table3.csv<br> Table 3. Statistical analysis of variance for
 EE in Box-Behnken Design <br> dataset:
 F.B.H-Table4.csv<br> Table 4. Statistical analysis of variance for
 DL in Box-Behnken Design<br> <br> dataset:
 F.B.H-Table5.csv<br> Table 5. Statistical analysis of variance for
 size in Box-Behnken Design<br> <br> dataset:
 F.B.H-Table6.csv<br> Table 6. Optimized values obtained by
 constraints applied on EE， DL, Size.<br> <br> dataset:
 F.B.H-Figure2sizedata.csv                                                
                                                                          
                                             Raw data of
 experiments with size dataset:F.B.H-Figure3DSCdata.csv
                                                                          
                                                                          
                   Raw data of experiments with differential
 scanning calorimetry
 dataset:F.B.H-Figure3FTIRdata.csv                               
                                                                          
                                                              
 Raw data of experiments with Fourier Transform Infrared
 Spectroscopic
 dataset:F.B.H-Figure3TGdata.csv                                 
                                                                          
                                                            
 Raw data of experiments with thermo-gravimetric
 analyzer    
 dataset:F.B.H-Figure3XRDdata.csv                                
                                                                          
                                                            
 Raw data of experiments with X-ray Diffraction Patterns          
                                                                          
                                                                  
   <br> dataset:F.B.H-Figure4celluptakedata.csv
 Raw data of FACS experiments with HaCaT cell uptake
 dataset: F.B.H-Figure5MTT24hdata.csv;
 F.B.H-Figure5MTT48hdata.csv; F.B.H-Figure5UVBMTTdata.csv
 Raw data of MTT experiments with HaCaT cell viability in
 different time dataset:
 F.B.H-Figure6ROSdata.CSV Raw data of FACS experiments
 with ROS dataset: F.B.H-Figure7JC1ASTdata.csv
 Raw data of FACS experiments with JC1 for Astaxanthin<br>
 <br> dataset: F.B.H-Figure7JC1AST-PLGANPdata.csv
 Raw data of FACS experiments with JC1 for PLGA nanoparticles
 loaded with Astaxanthin
 dataset:F.B.H-Figure4celluptakeconfocal                          
                                                                          
                                                    
  Image of cellular uptake of the nanoparticles on Figure
 4C dataset:F.B.H-Figure6ROSconfocal                    
                                                                          
                                                                
    Image of the ROS level  in cells on Figure 6C
    dataset:F.B.H-Figure7JC1confocal                    
                                                                          
                                                                  
   Image of <i> </i>ΔΨm in cells on Figure
 7C