DOI: 10.5061/dryad.31zcrjdrp
title: High specificity of symbiont-conferred resistance in an aphid-parasitoid field community

description: Host-parasite coevolution is mediated by genetic interactions between the
 antagonists and may lead to reciprocal adaptation. In the black bean
 aphid, Aphis fabae fabae, resistance to parasitoids can be conferred by
 the heritable bacterial endosymbiont Hamiltonella defensa. H. defensa has
 been shown to be variably protective against different parasitoid species,
 and different genotypes of the black bean aphid’s main parasitoid
 Lysiphlebus fabarum. However, these results were obtained using haphazard
 combinations of laboratory-reared insect lines with different origins,
 making it unclear how representative they are of natural, locally
 (co)adapted communities. We therefore comprehensively sampled the
 parasitoids of a natural A. f. fabae population and measured the ability
 of the five most abundant species to parasitize aphids carrying the
 locally prevalent H. defensa haplotypes. H. defensa provided resistance
 only against the dominant parasitoid L. fabarum (70% of all parasitoids),
 but not against less abundant parasitoids, and resistance to L. fabarum
 acted in a genotype-specific manner (G × G interactions between H. defensa
 and L. fabarum). These results confirm that strong species- and
 genotype-specificity of symbiont-conferred resistance is indeed a hallmark
 of wild A. f. fabae populations, and they are consistent with
 symbiont-mediated local adaptation of aphids to parasitoids.
description: See manuscript.
description: The data submission contains: a
 .csv file containing all experimental data a README
 containing information to understand the data file a
 .R file containing the code to reproduce the statistical analyses and
 figures presented in the manuscript
description: # High specificity of symbiont-conferred resistance in an aphid-parasitoid
 field community Authors:
 Elena Gimmi & Christoph Vorburger Title: High specificity of
 symbiont-conferred resistance in an aphid-parasitoid field community
 submitted to Proceedings of the Royal Society B on June 20, 2023 The study
 design is explained in the manuscript. For questions please contact
 
 \############################################# This data submission
 contains two files: analysis_wasp_project_Gimmi_Vorburger.R
 data_Gimmi_Vorburger.csv \#############################################
 The file analysis_wasp_project_Gimmi_Vorburger.R contains the R code to
 run all analyses. The file data_Gimmi_Vorburger.csv contains all data
 discussed in the paper and has 7 columns: id: Unique identifier for the
 experimental unit, that is, one potted plant. The id is a combination of
 block (left side of the point) and position within the block (right side
 of the point). There are 240 samples, i.e. 240 ids. block: 1 out of 10
 randomized complete blocks. Each block consisted of 1 plastic tray
 containing a total of 24 potted and singly covered plants, i.e.
 experimental units. aphid: Aphid line. All aphids were genetically
 identical and differed only in their Hamiltonella infection state:
 uninfected (a407), infected with Hamiltonella H42 (a407H42) or infected
 with Hamiltonella H76 (a407H76) wasp: Parasitoid species or line. Acha
 (Aphelinus chaonia), Bang (Binodoxys angelicae), Epla (Ephedrus
 palliator), Pvol (Praon voluter), Lfab1 (Lysiphlebus fabarum line 1),
 Lfab2 (L. fabarum line 2), Lfab3 (L. fabarum line 3), Lfab5 (L. fabarum
 line 5). nr_aphids_day_3: Number of aphid nymphs counted per plant on day
 3 of the experiment. nr_mummies_day_16: Number of mummies counted per
 plant on day 16 of the experiment. nr_wasps_hatched: Number of parasitoids
 hatched from the mummies collected on day 16.
 \#############################################