DOI: 10.5061/dryad.dm5p030
title: Data from: Energy demand and the context-dependent effects of genetic interactions underlying metabolism

description: Genetic effects are often context-dependent, with the same genotype
 differentially affecting phenotypes across environments, life stages, and
 sexes. We used an environmental manipulation designed to increase energy
 demand during development to investigate energy demand as a general
 physiological explanation for context-dependent effects of mutations,
 particularly for those mutations that affect metabolism. We found that
 increasing the period during which Drosophila larvae are active during
 development phenocopies a temperature-dependent developmental delay in a
 mitochondrial-nuclear genotype with disrupted metabolism. This result
 indicates that the context-dependent fitness effects of this genotype are
 not specific to the effects of temperature and may result generally from
 variation in energy demand. The effects of this genotype also differ
 across life stages and between the sexes. Metabolic rates are disrupted by
 this genetic interaction in growing larvae, but not in adults; and
 reproduction of females, but not males, is compromised by this genetic
 interaction. These patterns are consistent with a model where
 context-dependent genotype-phenotype relationships may generally arise
 from differences in energy demand experienced by individuals across
 environments, life stages, and sexes.
description: development time
 dataFile 1: devtime.xlsx 5
 data sheets Eclose16_LD.txt Eclose16_LL.txt Eclose22_LL.txt
 Eclose22_LD.txt Eclose25_LD.txt Rows: each row represents a single adult
 that eclosed on a given date with a particular development time in days
 (“EcloseDay”) Columns: Date — date vial was scored for progeny; Mito —
 mtDNA genotype; Nuc — nuclear genotype; Temp — rearing temperature; Light
 — photoperiod; 12,12 (LD) or 24,0 (LL); Vial — unique identifier for each
 vial; EcloseDay — days until eclosion;devtime.xlsxmetabolic rate dataFile 2: metrate.xlsx 4 data sheets MRdat_Group1616.txt MRdat_Group2525.txt MRdat_Group1625.txt MRdat_Group2516.txt Rows: each row represents a measure of average VCO2 from a chamber containing 10 adult flies Columns date — date of measurement; run — unique run of the respirometry system; rep — each chamber was sampled twice during the run; line — (mtDNA);nuclear genotype; mtDNA — mtDNA genotype; nuclear — nuclear genotype; tdev - development rearing temperature in C; tmeasure - temperature in C at which metabolic rate was measured; sex; CO2 — average rate of CO2 production; CO2.se — standard error of this average; mass — mass of 10 flies in chamber; MassMeasured — the initial mass of 10 flies corrected for any flies lost during the experiment (total mass - mean mass * number of flies lost); Act1 — the median absolute difference of the squared activity signal; Act2 — the standard error of the squared activity signalmetrate.xlsxreproduction dataFile 3: reproduction.xlsx datasheet: mitofert.txt Rows: each row represents a single male’s number of offspring sired across three females Columns mtDNA — mtDNA genotype; nuclear — nuclear genotype; genotype — mtDNA;nuclear genotype; male — replicate male ID; females — number of females who survived and produced offspring; total — the sum of offspring for each male across the three females; totalper — total divided by the number of females; exp — block A or B datasheet: mitofecund.txt Rows: each row represents the number of eggs laid by each female (original data are reported in Meiklejohn et al. (2013) PLoS Genet 9:e1003238) Columns mtDNA — mtDNA genotype; nuclear — nuclear genotype; genotype — mtDNA.nuclear genotype; female — female ID number; day1-day10 — egg counts each day; total — summed egg counts across all daysreproduction.xlsx