cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7513658
title: SOMATIC CELLS LEVEL ON THE PHYSICOCHEMICAL PROFILE OF POWDERED MILK

description: Abstract The objective of this study was to evaluate the level of somatic cells (SCC) on the physical-chemical profile of powdered milk. From January 2010 to June 2011, we analyzed seven raw milk samples and the respective powdered milk, totaling 14 samples from an industry in São Luís de Montes Belos-GO. Raw milk was analyzed for somatic cell counting and powdered milk for physical and chemical characteristics: acidity, moisture, protein, and fat. Two treatments were evaluated: powdered milk made from milk containing SCC ≤ 400,000 cells/mL and powdered milk prepared with SCC &gt; 400,000 cells/mL. The experimental design was in randomized blocks. For the statistical analysis we used analysis of variance, followed by Tukey test, considering p &lt; 0.05 as the minimum acceptable probability for the difference between means. The powdered milk prepared with raw material with SCC superior to 400,000 cells/mL presented lower protein and higher humidity content. Considering the treatments, we can state that to obtain powder milk satisfactorily, it is necessary to use milk with SCC lower than 400,000 cells/mL.
DOI: 10.6084/m9.figshare.7513658.v1
title: SOMATIC CELLS LEVEL ON THE PHYSICOCHEMICAL PROFILE OF POWDERED MILK

description: Abstract The objective of this study was to evaluate the level of somatic cells (SCC) on the physical-chemical profile of powdered milk. From January 2010 to June 2011, we analyzed seven raw milk samples and the respective powdered milk, totaling 14 samples from an industry in São Luís de Montes Belos-GO. Raw milk was analyzed for somatic cell counting and powdered milk for physical and chemical characteristics: acidity, moisture, protein, and fat. Two treatments were evaluated: powdered milk made from milk containing SCC ≤ 400,000 cells/mL and powdered milk prepared with SCC &gt; 400,000 cells/mL. The experimental design was in randomized blocks. For the statistical analysis we used analysis of variance, followed by Tukey test, considering p &lt; 0.05 as the minimum acceptable probability for the difference between means. The powdered milk prepared with raw material with SCC superior to 400,000 cells/mL presented lower protein and higher humidity content. Considering the treatments, we can state that to obtain powder milk satisfactorily, it is necessary to use milk with SCC lower than 400,000 cells/mL.
DOI: 10.6084/m9.figshare.7514612
title: SANITARY-HYGIENIC QUALITY OF FRESH AND FROZEN TILAPIA (Oreochromis spp.) IN PUBLIC MARKETS

description: Abstract This study aimed to verify the sanitary quality of tilapia (Oreochromis spp.) sold in public markets in the city of Mossoro-RN. For such we made use of a checklist and an observational study to evaluate good handling practices in 10 establishments that commercialize fish. We obtained 30 samples of tilapia and evaluated them as to their sensory and microbiological aspects (Salmonella spp., coliforms at 45 ºC, Staphylococcus ssp. count, psychrotrophic bacteria and enterobacteria), as well as to their pH and internal and external temperature. We observed that none of the studied establishments were in accordance with the standards recommended by the legislation. Regarding the sensory evaluation, 83.3% of the samples showed compliance with the requirements of the legislation. As for the coliforms at 45 °C essay, the results ranged from 0.477 to 1.509 log10 CFU/g; in the Staphylococcus spp. essay we found results from 4.854 to 5.397 log10 CFU/g; and further, we found values from 3.884 to 4.937 and from 4.707 to 5.463 log10 CFU/g for enterobacteria and psychrotrophic bacteria, respectively. All the samples were within the requirements for contamination by Salmonella spp. (absence). The pH ranged from 5.99 to 6.18 and temperatures indicated non-compliance with the recommendations. Thus, we can infer that the sanitary quality of tilapia sold in public markets in Mossoro-RN was impaired, presenting a risk to consumer health due to unsatisfactory handling conditions.
DOI: 10.6084/m9.figshare.7514612.v1
title: SANITARY-HYGIENIC QUALITY OF FRESH AND FROZEN TILAPIA (Oreochromis spp.) IN PUBLIC MARKETS

description: Abstract This study aimed to verify the sanitary quality of tilapia (Oreochromis spp.) sold in public markets in the city of Mossoro-RN. For such we made use of a checklist and an observational study to evaluate good handling practices in 10 establishments that commercialize fish. We obtained 30 samples of tilapia and evaluated them as to their sensory and microbiological aspects (Salmonella spp., coliforms at 45 ºC, Staphylococcus ssp. count, psychrotrophic bacteria and enterobacteria), as well as to their pH and internal and external temperature. We observed that none of the studied establishments were in accordance with the standards recommended by the legislation. Regarding the sensory evaluation, 83.3% of the samples showed compliance with the requirements of the legislation. As for the coliforms at 45 °C essay, the results ranged from 0.477 to 1.509 log10 CFU/g; in the Staphylococcus spp. essay we found results from 4.854 to 5.397 log10 CFU/g; and further, we found values from 3.884 to 4.937 and from 4.707 to 5.463 log10 CFU/g for enterobacteria and psychrotrophic bacteria, respectively. All the samples were within the requirements for contamination by Salmonella spp. (absence). The pH ranged from 5.99 to 6.18 and temperatures indicated non-compliance with the recommendations. Thus, we can infer that the sanitary quality of tilapia sold in public markets in Mossoro-RN was impaired, presenting a risk to consumer health due to unsatisfactory handling conditions.
DOI: 10.6084/m9.figshare.7516070
title: ACCURACY OF OSCILLOMETRIC (PETMAPTM) AND DOPPLER METHODS TO INDIRECT MEASUREMENT OF BLOOD PRESSURE IN LAMBS

description: Abstract Neonatal physiology has peculiarities inherent to the age group. The objective of this study was to monitor the systemic arterial pressure in lambs during neonatal period. We used 20 Ile de France lambs, from birth and at 7, 14, 21, 28 and 35 days of life. The following parameters were analyzed: heart rate (HR), systolic (SBP), diastolic (DBP), and average blood pressure (ABP) by oscillometric method petMAPTM, and SBP with Doppler. Invasive pressure validated indirect methods with the average 101.52 ± 12.04 mmHg. The averages with petMAPTM were as follows: HR (156.38 ± 37.46 bpm); DBP (63.80 ± 11.14 mmHg); ABP (81.58 ± 11.83 mmHg); SBP (112.48 ± 15.68 mmHg) and SBP by Dobler (90.27 ± 12.11 mmHg). There was significant difference in HR and blood pressure among the moments. Indirect methods differ between each other in 12.30 mmHg (overestimation of 11%). Comparing with the invasive methods, both overestimated values of 4% and 16% in PAS, respectively, for Doppler and petMAPTM. The results showed that the Doppler method has established a good relationship with the invasive one being useful for gauging the SBP. The oscillometric method requires larger studies to be used in small ruminants.
DOI: 10.6084/m9.figshare.7516070.v1
title: ACCURACY OF OSCILLOMETRIC (PETMAPTM) AND DOPPLER METHODS TO INDIRECT MEASUREMENT OF BLOOD PRESSURE IN LAMBS

description: Abstract Neonatal physiology has peculiarities inherent to the age group. The objective of this study was to monitor the systemic arterial pressure in lambs during neonatal period. We used 20 Ile de France lambs, from birth and at 7, 14, 21, 28 and 35 days of life. The following parameters were analyzed: heart rate (HR), systolic (SBP), diastolic (DBP), and average blood pressure (ABP) by oscillometric method petMAPTM, and SBP with Doppler. Invasive pressure validated indirect methods with the average 101.52 ± 12.04 mmHg. The averages with petMAPTM were as follows: HR (156.38 ± 37.46 bpm); DBP (63.80 ± 11.14 mmHg); ABP (81.58 ± 11.83 mmHg); SBP (112.48 ± 15.68 mmHg) and SBP by Dobler (90.27 ± 12.11 mmHg). There was significant difference in HR and blood pressure among the moments. Indirect methods differ between each other in 12.30 mmHg (overestimation of 11%). Comparing with the invasive methods, both overestimated values of 4% and 16% in PAS, respectively, for Doppler and petMAPTM. The results showed that the Doppler method has established a good relationship with the invasive one being useful for gauging the SBP. The oscillometric method requires larger studies to be used in small ruminants.
DOI: 10.6084/m9.figshare.7509146
title: BIOCHEMISTRY CHARACTERIZATION OF EXCRETION / SECRETION PRODUCT OF Cochliomyia hominivorax LARVAE (DIPTERA : CALLIPHORIDAE)

description: Abstract The species Cochliomyia hominivorax, also known as screwworm fly, is an obligate parasite of warm-blooded animals and its geographic range extends thoughout South America, except Chile. This fly causes significant economic losses and has great importance in Brazil. Few studies have focused on the excretion and secretion products of this species, and this research aimed to study the enzymes present in the secretion and excretion (E/S) products of the three larval instars of C. hominivorax. The E/S profile of proteins was obtained by polyacrylamide gel electrophoresis and proteolytic activity was analyzed using gelatin, azocasein and Na-benzoyl-arginine-nitroanilide as substrates. In E/S products of the three instars, proteins were detected with an apparent molecular weight ranging between 116 and 20 kDa. In the azocasein assay, at different pH ranges, the major proteolytic activity occurred at pH 7.5 for all larval instars. Assays were performed using the same substrates in which the samples were treated with the inhibitors Benzamidine, Pepstatin A, 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF), N-α-tosyl-L-lysine chloromethyl ketone (TLCK), N-α- tosyl-L-phenylalanine chloromethyl ketone (TPCK), Ethylenediamine tetraacetic acid (EDTA), and Leupeptin-trans-Epoxysuccinyl-leucylamido(4-guanidino) butane (E-64). Proteinases present in the E/S product of first larvae instar are mostly serine trypsin and chymotrypsin proteases, whereas for second and third instars serine proteases and aspartyl proteases were predominantly observed. Biochemical characterization of E/S products of all larval stages of C. hominivorax helps to improve the understanding of the physiology and the interaction of this parasite with host tissues.
DOI: 10.6084/m9.figshare.7509146.v1
title: BIOCHEMISTRY CHARACTERIZATION OF EXCRETION / SECRETION PRODUCT OF Cochliomyia hominivorax LARVAE (DIPTERA : CALLIPHORIDAE)

description: Abstract The species Cochliomyia hominivorax, also known as screwworm fly, is an obligate parasite of warm-blooded animals and its geographic range extends thoughout South America, except Chile. This fly causes significant economic losses and has great importance in Brazil. Few studies have focused on the excretion and secretion products of this species, and this research aimed to study the enzymes present in the secretion and excretion (E/S) products of the three larval instars of C. hominivorax. The E/S profile of proteins was obtained by polyacrylamide gel electrophoresis and proteolytic activity was analyzed using gelatin, azocasein and Na-benzoyl-arginine-nitroanilide as substrates. In E/S products of the three instars, proteins were detected with an apparent molecular weight ranging between 116 and 20 kDa. In the azocasein assay, at different pH ranges, the major proteolytic activity occurred at pH 7.5 for all larval instars. Assays were performed using the same substrates in which the samples were treated with the inhibitors Benzamidine, Pepstatin A, 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF), N-α-tosyl-L-lysine chloromethyl ketone (TLCK), N-α- tosyl-L-phenylalanine chloromethyl ketone (TPCK), Ethylenediamine tetraacetic acid (EDTA), and Leupeptin-trans-Epoxysuccinyl-leucylamido(4-guanidino) butane (E-64). Proteinases present in the E/S product of first larvae instar are mostly serine trypsin and chymotrypsin proteases, whereas for second and third instars serine proteases and aspartyl proteases were predominantly observed. Biochemical characterization of E/S products of all larval stages of C. hominivorax helps to improve the understanding of the physiology and the interaction of this parasite with host tissues.
DOI: 10.6084/m9.figshare.7506959.v1
title: FREQUENCY OF ANTI-TOXOPLASMA GONDII ANTIBODIES IN DOGS WITH CLINICAL SIGNS CONSISTENT WITH TOXOPLASMOSIS

description: Abstract: The aim of this study was to detect antibodies against T. gondii in dogs with clinical signs compatible with toxoplasmosis attended in a veterinary hospital. A serological survey was performed in 598 dogs from 1993 to 2013. The IgG antibodies survey against T. gondii was carried out by means of Indirect Fluorescence Antibodies Test (IFAT); samples with titers ≥16 were considered positive. Among the dogs with clinical signs, 259 (43,31%) were positive for T. gondii with titers varying between 16 to 4096; among the healthy animals, 79 (22.25%) were reagents. The most frequent signals were: neurological (30.43%) and uveitis (8.03%). Among variables race, gender, and age, only the last one affected the observed results with greater frequency f seropositive in elderly dogs.
DOI: 10.6084/m9.figshare.7517801
title: ULTRASONOGRAPHIC CHARACTERISTICS AND MEASUREMENTS OF SPLEEN OF HEALTHY PUPPIES AND KITTENS

description: Abstract The aim of the study was to analyze and compare the characteristics and ultrasound measurements of the spleen of healthy puppies and kittens, establishing normal standards and landmarks. We used 15 healthy male dogs and 15 healthy male cats, mixed breed and average age of six months. They were divided into two groups: G1 with 15 puppies with an average weight of 3 kg and G2 with 15 kittens with an average weight of 2 kg. The animals underwent ultrasound examination of the spleen for visualization of internal characteristics and measurement of the organ. The structural characteristics was analyzed and compared between the two species through the images obtained in the exam. The measurements were compared statistically using the SPSS program, with analysis of variance (ANOVA) followed by Tukey test (P ˂ 0.05). In both groups, we observed the splenic artery showed smaller diameter than the splenic veins. In puppies and kittens, the echotexture was visualized homogeneous and finely granular. The puppy's spleen tends to be bigger in length (6.87 ± 0.03 cm) and width (5.95 ± 0.07 cm) in relation to spleen length (4,53 ± 0,02 cm)) and width (4.55 ± 0.04) in kittens. We concluded there is no difference of echotexture and splenic echogenicity between puppies and kittens, and the spleen of puppies showed bigger dimensions than in kittens.
DOI: 10.6084/m9.figshare.7517801.v1
title: ULTRASONOGRAPHIC CHARACTERISTICS AND MEASUREMENTS OF SPLEEN OF HEALTHY PUPPIES AND KITTENS

description: Abstract The aim of the study was to analyze and compare the characteristics and ultrasound measurements of the spleen of healthy puppies and kittens, establishing normal standards and landmarks. We used 15 healthy male dogs and 15 healthy male cats, mixed breed and average age of six months. They were divided into two groups: G1 with 15 puppies with an average weight of 3 kg and G2 with 15 kittens with an average weight of 2 kg. The animals underwent ultrasound examination of the spleen for visualization of internal characteristics and measurement of the organ. The structural characteristics was analyzed and compared between the two species through the images obtained in the exam. The measurements were compared statistically using the SPSS program, with analysis of variance (ANOVA) followed by Tukey test (P ˂ 0.05). In both groups, we observed the splenic artery showed smaller diameter than the splenic veins. In puppies and kittens, the echotexture was visualized homogeneous and finely granular. The puppy's spleen tends to be bigger in length (6.87 ± 0.03 cm) and width (5.95 ± 0.07 cm) in relation to spleen length (4,53 ± 0,02 cm)) and width (4.55 ± 0.04) in kittens. We concluded there is no difference of echotexture and splenic echogenicity between puppies and kittens, and the spleen of puppies showed bigger dimensions than in kittens.
DOI: 10.6084/m9.figshare.7506959
title: FREQUENCY OF ANTI-TOXOPLASMA GONDII ANTIBODIES IN DOGS WITH CLINICAL SIGNS CONSISTENT WITH TOXOPLASMOSIS

description: Abstract: The aim of this study was to detect antibodies against T. gondii in dogs with clinical signs compatible with toxoplasmosis attended in a veterinary hospital. A serological survey was performed in 598 dogs from 1993 to 2013. The IgG antibodies survey against T. gondii was carried out by means of Indirect Fluorescence Antibodies Test (IFAT); samples with titers ≥16 were considered positive. Among the dogs with clinical signs, 259 (43,31%) were positive for T. gondii with titers varying between 16 to 4096; among the healthy animals, 79 (22.25%) were reagents. The most frequent signals were: neurological (30.43%) and uveitis (8.03%). Among variables race, gender, and age, only the last one affected the observed results with greater frequency f seropositive in elderly dogs.
DOI: 10.6084/m9.figshare.7516496
title: MORPHOMETRIC AND TESTICULAR CHARACTERISTICS OF BUFFALOS AT DIFFERENT AGES

description: Abstract We evaluated the live weight (LW), morphological and testicular measures and their correlations in 31 Mediterranean buffaloes at three ages. We used seven animals at 8 months of age (T1), 13 animals at the age of 10 months (T2), and 11 animals at the age of 12 months (T3) coming from a farm of the Coastal Lowlands of Rio de Janeiro. The following morphometric measurements and testes were evaluated: rump height (RH), withers height (WH), rump length (RL), round thickness (RT), scrotal circumference (SC), testicular length (TL), testicular width (TW). Testicular volume (TV) and body mass index (BMI) were also calculated. The testicular form was determined by the ratio between the TW and TL. We found the following means for the variables LW (240.57 ± 18.36; 259.38 ± 28.66; and 331.82 ± 63.23), WH (113.28 ± 2.44; 116.23 ± 4.74; and 121.45 ± 6.95cm), RH (116.07 ± 3.09; 117.54 ± 4.97; and 125.45 ± 6.92 cm), RL (34.86 ± 2.03; 36.35 ± 2.80; and 38.09 ± 3.24 cm), RT (39.07 ± 2.22; 40 ± 2.37; and 42.91 ± 3.97cm), BMI (187.37 ± 11.45; 191.69 ± 14.61; and 222.39 ± 23.57) regarding the treatments T1, T2, and T3, respectively. We found the following means for the variables SC (17.43±1.81; 19.08±1.98; and 22.11±3.25 cm), TL (8.28 ± 0.99; 9.27 ± 1.33; and 10.68 ± 1.47 cm), TW (3.25 ± 0.25; 3.90 ± 0.58; and 4.66 ± 0.80cm), and TV (109.43 ± 24.93; 198.36 ± 87.08; and 343.83 ± 183.04 cm3) regarding the treatments T1, T2, and T3, respectively. The predominant form in all treatments was long. There was no difference between the T1 and T2 for the studied variables. T3 had higher averages for LW, BMI, RH, SC, TV, and TW. For the variables WH, RL, TL, and RT there was no difference between T2 and T3. There was a correlation between measurements and testicular measures WH, RH, and RL and between SC and TV, TL, and TW.
DOI: 10.6084/m9.figshare.7516496.v1
title: MORPHOMETRIC AND TESTICULAR CHARACTERISTICS OF BUFFALOS AT DIFFERENT AGES

description: Abstract We evaluated the live weight (LW), morphological and testicular measures and their correlations in 31 Mediterranean buffaloes at three ages. We used seven animals at 8 months of age (T1), 13 animals at the age of 10 months (T2), and 11 animals at the age of 12 months (T3) coming from a farm of the Coastal Lowlands of Rio de Janeiro. The following morphometric measurements and testes were evaluated: rump height (RH), withers height (WH), rump length (RL), round thickness (RT), scrotal circumference (SC), testicular length (TL), testicular width (TW). Testicular volume (TV) and body mass index (BMI) were also calculated. The testicular form was determined by the ratio between the TW and TL. We found the following means for the variables LW (240.57 ± 18.36; 259.38 ± 28.66; and 331.82 ± 63.23), WH (113.28 ± 2.44; 116.23 ± 4.74; and 121.45 ± 6.95cm), RH (116.07 ± 3.09; 117.54 ± 4.97; and 125.45 ± 6.92 cm), RL (34.86 ± 2.03; 36.35 ± 2.80; and 38.09 ± 3.24 cm), RT (39.07 ± 2.22; 40 ± 2.37; and 42.91 ± 3.97cm), BMI (187.37 ± 11.45; 191.69 ± 14.61; and 222.39 ± 23.57) regarding the treatments T1, T2, and T3, respectively. We found the following means for the variables SC (17.43±1.81; 19.08±1.98; and 22.11±3.25 cm), TL (8.28 ± 0.99; 9.27 ± 1.33; and 10.68 ± 1.47 cm), TW (3.25 ± 0.25; 3.90 ± 0.58; and 4.66 ± 0.80cm), and TV (109.43 ± 24.93; 198.36 ± 87.08; and 343.83 ± 183.04 cm3) regarding the treatments T1, T2, and T3, respectively. The predominant form in all treatments was long. There was no difference between the T1 and T2 for the studied variables. T3 had higher averages for LW, BMI, RH, SC, TV, and TW. For the variables WH, RL, TL, and RT there was no difference between T2 and T3. There was a correlation between measurements and testicular measures WH, RH, and RL and between SC and TV, TL, and TW.
DOI: 10.6084/m9.figshare.7516502
title: EVALUATION OF THE GENETIC COMPONENT IN THE PHENOTYPIC EXPRESSION OF PRODUCTION TRAITS IN NELLORE CATTLE SUBMITTED TO PERFORMANCE TEST

description: Abstract The objective of this study was to evaluate the effect of the genetic component on the phenotypic expression of productive traits of Nellore cattle submitted to the individual performance test. The data used came from 51 young bulls participating in the individual performance test of Nelore bulls held at the Capim Branco experimental farm of the Federal University of Uberlândia. The evaluated traits were weight and scrotal circumference standardized at 365 and at 450 days of age, the longissimus muscle area, and the backfat thickness. The expected progeny differences of the animals and the sires were considered to evaluate the contribution of the genetic component. Variance analysis was performed through the General Linear Model procedure of the Statistical Analysis System to verify the effects of the genetic groups on the productive performance. The progenies of bulls with greater genetic potential tend to present better productive performance. In conclusion, the use of genetically superior animals allows better zootechnical indexes to be obtained, reflecting higher gains in herd productivity.
DOI: 10.6084/m9.figshare.7516502.v1
title: EVALUATION OF THE GENETIC COMPONENT IN THE PHENOTYPIC EXPRESSION OF PRODUCTION TRAITS IN NELLORE CATTLE SUBMITTED TO PERFORMANCE TEST

description: Abstract The objective of this study was to evaluate the effect of the genetic component on the phenotypic expression of productive traits of Nellore cattle submitted to the individual performance test. The data used came from 51 young bulls participating in the individual performance test of Nelore bulls held at the Capim Branco experimental farm of the Federal University of Uberlândia. The evaluated traits were weight and scrotal circumference standardized at 365 and at 450 days of age, the longissimus muscle area, and the backfat thickness. The expected progeny differences of the animals and the sires were considered to evaluate the contribution of the genetic component. Variance analysis was performed through the General Linear Model procedure of the Statistical Analysis System to verify the effects of the genetic groups on the productive performance. The progenies of bulls with greater genetic potential tend to present better productive performance. In conclusion, the use of genetically superior animals allows better zootechnical indexes to be obtained, reflecting higher gains in herd productivity.