cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.60909/kxlhlw
title: Bipartite quantitative matrix of pollination interactions in the Matarredonda páramo

description: The database contains records of pollinator visits to flowers of 36 angiosperm species during 2021, 2022, and 2023 in the Matarredonda páramo, located in the Eastern Cordillera of Colombia. This frequency matrix shows the morphospecies of pollinators in the columns and the studied plant species in the rows. In total, the database includes 333 interactions, reporting 90 pollinator morphospecies, including hummingbirds, bats, hymenoptera, diptera, lepidoptera, coleoptera, and hemiptera. Many of the reported interactions may be new for the páramo, increasing our understanding of pollination interactions in the ecosystem. This allows us to continue studying the ecology of flowering plants in Colombian páramos and generate new research questions. Additionally, this data helped construct the first pollination network for Colombian páramos, which could serve as a foundation for future monitoring efforts. Finally, with this information, future management plans can be developed to conserve the páramo and its species.
description: &lt;br&gt;La base de datos contiene los registros de visita de polinizadores a flores de 36 especies de angiospermas durante el 2021, 2022 y 2023 en el páramo de Matarredonda, ubicado en la cordillera oriental de Colombia. Esta matriz de frecuencias muestra en las columnas las morfoespecies de polinizadores, en las filas las especies de plantas estudiadas. En total esta base de datos posee 333 interacciones, reportando 90 morfoespecies de polinizadores entre ellos colibríes, murciélagos, himenópteros, dópteros, lepidópteros, coleopteros y hemópteros. Muchas interacciones de las reportadas pueden ser nuevas para el páramo, lo cual aumenta el conocimiento de las interacciones de polinización en el ecosistema, permitiendo continuar con el estudio de la ecología de las plantas con flor de los páramos Colombianos y generando nuevas preguntas a su alrededor. Además, con ellos se construyó la primera red de polinización para el páramo colombiano con la cual se podría tomar como base y realizar monitoreos de las mismas en el futuro. Por último, con esta información se pueden realizar planes de manejo a futuro en pro de la conservación del páramo y sus especies.&lt;/br&gt;
DOI: 10.60909/kxlhlw/mpirwg
title: Bipartite quantitative matrix of pollination interactions in the Matarredonda páramo (1).tab

description: Matriz de las interacciones de polinización del páramo de Matarredonda. Estos datos son de observaciones de 36 especies de plantas tomados en el 2021, 2022 y 2023.
DOI: 10.15468/dl.unxk8n
title: Occurrence Download

description: A dataset containing 87 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Ficus bonijesulapensis R.M.Castro"
 ]
}

 The dataset includes 87 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008237-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5519/qd.nelssmy8
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (74 records)
DOI: 10.15468/dl.qa2cvu
title: Occurrence Download

description: A dataset containing 1415 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Observation, Human Observation, Machine Observation, Living Specimen)"
 ]
 }
 },
 "TaxonKey is Nomada fucata Panzer, 1798"
 ]
}

 The dataset includes 1415 records from 33 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008238-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5519/qd.lvdfwbqk
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (87 records)
DOI: 10.6084/m9.figshare.28904477
title: Preliminary single cell sequencing data from "Telocytes and CCL24+ macrophages do not contribute to TSLP responses during high dose <i>Trichuris muris</i> infection."

description: Infected mice, all age-matched C57BL/6 males, were administered 400 <i>Tm</i> eggs by oral gavage: four mice were infected for harvest at d14 p.i., four mice for harvest at d21 p.i., and four remained uninfected. Caeca were harvested from all mice simultaneously, washed in PBS, and the epithelia stripped <i>via</i> two, 30 minute incubations in 10mL of strip buffer per sample (HBSS -Mg/Ca + 5mM EDTA + 1mM DTT (Sigma), 5% FCS), before vortexing samples and separating lamina propria from suspended epithelial cells through a 100µm filter. Epithelial cell suspensions from mice of the same infection timepoint were combined centrifuged at 400xg at 4ºC for 10 minutes, resuspended in PBS and kept on ice. Lamina propria samples were combined, and each of the three grouped samples digested enzymatically for 30min at 37ºC in a shaking shaking incubator. Digestion buffer was comprised of HBSS +Mg/Ca +1mg/mL of Liberase TL +10mg/mL DNAse. Digested samples were centrifuged at 400xg at 4ºC for 10 minutes, resuspended in chilled PBS buffer. This was repeated to wash samples. Cells were stained using Zombie UV fixable <i>via</i>bility dye and sorted for live cells using the Bigfoot Cell Sorter (Thermo Fisher). The concentrations of cells for the three sorted lamina propria samples, and the three epithelial samples, were determined <i>via</i> CASY counter, and for each group a sample was generated containing 8,000 lamina propria cells and 2,000 epithelial cells in EDTA-free FACS buffer, which was deposited with the Genomics Core Facility for 10x single cell sequencing. FASTQ files generated were subjected to quality control and three resolutions of clustering and annotation by the Bioinformatics Core Facility, who provided the R-scripts used for quality control, .csv files containing cell clustering annotations against cell barcode, and total and log transformed reads for all transcripts against cell barcodes, as well as access to the data loaded onto the Shiny App for examination of data. Data presented here uses Prism-generated graphs of data from quality controlled .csv files at the finest cluster resolution provided by the Bioinformatics Core Facility. The gene list given has been restricted to genes graphed in the related manuscript, with genes for validation of clustering provided, for potential use in subsequent research by authors and collaborators.
DOI: 10.6084/m9.figshare.28901954
title: Doc1.docx

description: The Infectious pancreatic necrosis virus (IPNV) belongs to the Birnaviridae family and the Aquabirnavirus genus, with salmonid fish as its primary host. Using vector-based shRNA molecules against the target genes has presented a new brand and effective therapeutic approach in using the RNAi pathway. Therefore, in the present study, the appropriate oligonucleotide sequence for encoding the gene of the generator gene of RNA polymerase of the IPNV virus was chosen, using the NCBI database. The designed shRNA structures were validated by online shRNA design software and further confirmed by blasting them against the fish genome in the NCBI database. Subsequently, shRNA oligonucleotides were synthesized and cloned into a lentiviral plasmid. Lentiviruses containing shRNA were produced in HEK 293T cells using a lentiviral packaging system. Following this, the lentivectors expressing shRNA were introduced into the CS2-2 cell line infected with the IPNV virus. The effectiveness of the designed shRNA molecules in suppressing the expression of the viral RNA polymerase gene was evaluated using the TCID<sub>50</sub> test. The results indicated a significant reduction in viral titer, up to 99.9%, suggesting the potential of shRNA as a defense tool against IPNV virus in cell culture applications. This study highlights the promising role of shRNA technology in combating viral infections, explicitly targeting IPNV in this context.
DOI: 10.6084/m9.figshare.26779128
title: A qualitative study investigating the experiences of unmet social needs for children with cerebral palsy and their families: perspectives of parents and clinicians

description: To explore (i) the impact of unmet social needs on children with cerebral palsy and their families; (ii) enablers-, and (iii) barriers to addressing unmet social needs. Eligible participants attended or worked at one of the three Paediatric Rehabilitation Departments including: children with a diagnosis of cerebral palsy; parents/carers; and clinicians. One-on-one interviews were conducted with parents/carers and focus groups with clinicians. Interview and focus group transcripts were deductively thematically analysed according to the social model of disability. A total of 44 participants (8 parents and 36 clinicians) took part. No children consented to participate. Analysis of the qualitative data identified four main themes and 14 sub-themes. The main themes were: Unmet social needs are pervasive; An inequitable health system with no roadmap; Everyone suffers as a result of unmet social needs; and It takes a village to raise a child. Unmet social needs have profound impacts on families. The experiences of unmet social needs are intensified by the extra complexities of raising a child with disability. Societal barriers including inequitable systems and the fragmented services are barriers impeding on families receiving support and ultimately limiting their wellbeing. Many families experience a vicious cycle of disability, unmet social needs, and access – which service providers should thoughtfully consider when providing patient-centred care.For many families, a child’s disability impacts their unmet social needs, which influences their access to services and has consequences on their disability and wellbeing.Addressing unmet social needs is a priority for all people working with families of children with cerebral palsy including health, social care, and education providers.Integrated health-social care models such as social prescribing have the potential to support families to address their unmet social needs and warrant consideration within rehabilitation care. Many families experience a vicious cycle of disability, unmet social needs, and access – which service providers should thoughtfully consider when providing patient-centred care. For many families, a child’s disability impacts their unmet social needs, which influences their access to services and has consequences on their disability and wellbeing. Addressing unmet social needs is a priority for all people working with families of children with cerebral palsy including health, social care, and education providers. Integrated health-social care models such as social prescribing have the potential to support families to address their unmet social needs and warrant consideration within rehabilitation care.
DOI: 10.6084/m9.figshare.26779128.v1
title: A qualitative study investigating the experiences of unmet social needs for children with cerebral palsy and their families: perspectives of parents and clinicians

description: To explore (i) the impact of unmet social needs on children with cerebral palsy and their families; (ii) enablers-, and (iii) barriers to addressing unmet social needs. Eligible participants attended or worked at one of the three Paediatric Rehabilitation Departments including: children with a diagnosis of cerebral palsy; parents/carers; and clinicians. One-on-one interviews were conducted with parents/carers and focus groups with clinicians. Interview and focus group transcripts were deductively thematically analysed according to the social model of disability. A total of 44 participants (8 parents and 36 clinicians) took part. No children consented to participate. Analysis of the qualitative data identified four main themes and 14 sub-themes. The main themes were: Unmet social needs are pervasive; An inequitable health system with no roadmap; Everyone suffers as a result of unmet social needs; and It takes a village to raise a child. Unmet social needs have profound impacts on families. The experiences of unmet social needs are intensified by the extra complexities of raising a child with disability. Societal barriers including inequitable systems and the fragmented services are barriers impeding on families receiving support and ultimately limiting their wellbeing. Many families experience a vicious cycle of disability, unmet social needs, and access – which service providers should thoughtfully consider when providing patient-centred care.For many families, a child’s disability impacts their unmet social needs, which influences their access to services and has consequences on their disability and wellbeing.Addressing unmet social needs is a priority for all people working with families of children with cerebral palsy including health, social care, and education providers.Integrated health-social care models such as social prescribing have the potential to support families to address their unmet social needs and warrant consideration within rehabilitation care. Many families experience a vicious cycle of disability, unmet social needs, and access – which service providers should thoughtfully consider when providing patient-centred care. For many families, a child’s disability impacts their unmet social needs, which influences their access to services and has consequences on their disability and wellbeing. Addressing unmet social needs is a priority for all people working with families of children with cerebral palsy including health, social care, and education providers. Integrated health-social care models such as social prescribing have the potential to support families to address their unmet social needs and warrant consideration within rehabilitation care.
DOI: 10.6084/m9.figshare.26840813
title: Effectiveness of trigger point manual therapy for rotator cuff related shoulder pain: a systematic review and meta-analysis

description: To evaluate the effectiveness of trigger point manual therapy (TPMT) in treating rotator cuff related shoulder pain (RCRSP). Randomized controlled trials that compared the effects of TPMT with no or other conservative treatments in patients with RCRSP were included. Primary outcomes were shoulder pain intensity and function. Secondary outcomes were pressure pain threshold (PPT) and number of myofascial trigger points (MTrPs). The Cochrane Risk of Bias 2.0 tool, PEDro scale and GRADE approach were employed. Ten studies were included in this systematic review and seven in the meta-analysis. Very low to low quality of evidence showed no statistically significant difference between TPMT and other conservative treatments in rest and activity pain reduction in the short term (3 days to 12 weeks), and the difference in shoulder function was statistically significant in favor of TPMT. Furthermore, TPMT was found to be effective in the improvement of PPT and the inactivation of active MTrPs in the short term. TPMT may be equally effective as other passive treatments for the pain reduction in patients with RCRSP in the short term, and slightly more effective for functional improvement. TPMT seems to be effective to treat the active MTrPs in RCRSP. CRD42023409101 Trigger point manual therapy (TPMT) seems to be effective to treat the active trigger points of rotator cuff related shoulder pain (RCRSP) in the short term (3 days to 12 weeks).TPMT may be equally effective as other passive treatments for the pain reduction in patients with RCRSP in the short term (3 days to 12 weeks), and slightly more effective for functional improvement.TPMT may be recommended for use in comprehensive treatment programs which may include active components such as exercise therapy and education for the rehabilitation of RCRSP. Trigger point manual therapy (TPMT) seems to be effective to treat the active trigger points of rotator cuff related shoulder pain (RCRSP) in the short term (3 days to 12 weeks). TPMT may be equally effective as other passive treatments for the pain reduction in patients with RCRSP in the short term (3 days to 12 weeks), and slightly more effective for functional improvement. TPMT may be recommended for use in comprehensive treatment programs which may include active components such as exercise therapy and education for the rehabilitation of RCRSP.
DOI: 10.6084/m9.figshare.26840813.v1
title: Effectiveness of trigger point manual therapy for rotator cuff related shoulder pain: a systematic review and meta-analysis

description: To evaluate the effectiveness of trigger point manual therapy (TPMT) in treating rotator cuff related shoulder pain (RCRSP). Randomized controlled trials that compared the effects of TPMT with no or other conservative treatments in patients with RCRSP were included. Primary outcomes were shoulder pain intensity and function. Secondary outcomes were pressure pain threshold (PPT) and number of myofascial trigger points (MTrPs). The Cochrane Risk of Bias 2.0 tool, PEDro scale and GRADE approach were employed. Ten studies were included in this systematic review and seven in the meta-analysis. Very low to low quality of evidence showed no statistically significant difference between TPMT and other conservative treatments in rest and activity pain reduction in the short term (3 days to 12 weeks), and the difference in shoulder function was statistically significant in favor of TPMT. Furthermore, TPMT was found to be effective in the improvement of PPT and the inactivation of active MTrPs in the short term. TPMT may be equally effective as other passive treatments for the pain reduction in patients with RCRSP in the short term, and slightly more effective for functional improvement. TPMT seems to be effective to treat the active MTrPs in RCRSP. CRD42023409101 Trigger point manual therapy (TPMT) seems to be effective to treat the active trigger points of rotator cuff related shoulder pain (RCRSP) in the short term (3 days to 12 weeks).TPMT may be equally effective as other passive treatments for the pain reduction in patients with RCRSP in the short term (3 days to 12 weeks), and slightly more effective for functional improvement.TPMT may be recommended for use in comprehensive treatment programs which may include active components such as exercise therapy and education for the rehabilitation of RCRSP. Trigger point manual therapy (TPMT) seems to be effective to treat the active trigger points of rotator cuff related shoulder pain (RCRSP) in the short term (3 days to 12 weeks). TPMT may be equally effective as other passive treatments for the pain reduction in patients with RCRSP in the short term (3 days to 12 weeks), and slightly more effective for functional improvement. TPMT may be recommended for use in comprehensive treatment programs which may include active components such as exercise therapy and education for the rehabilitation of RCRSP.
DOI: 10.6084/m9.figshare.26860925
title: Association between upper limb clinical tests and accelerometry metrics for arm use in daily life in children with unilateral cerebral palsy

description: To evaluate the association between upper-limb (UL) clinical tests and UL accelerometry-derived metrics in children with unilateral Cerebral Palsy (CP). In this cross-sectional study, twenty children with unilateral CP and Manual Ability Classification System level I-III were included. Outcomes of the Assisting Hand Assessment, Box and Block-Test and accelerometry metrics were collected in the clinical setting and in daily life. UL asymmetry index (i.e., the ratio between the well-functioning UL and the affected UL use) was evaluated in different physical activity levels and relative use of UL was evaluated during daily living. Spearman’s correlation was used to determine the association between UL clinical tests and accelerometry metrics in a clinical setting and in daily life. The strongest negative association was between the Assisting Hand Assessment units and accelerometry metrics during the sedentary time in daily life (r<sub>s</sub> = −0.64). The asymmetries between ULs were highest during the child’s sedentary time (asymmetry index: 45.15) compared to when the child was in light (asymmetry index: 23.97) or higher intensity physical activity (asymmetry index: 13.39). The children used both ULs simultaneously for 44% of the time during daily life. Accelerometry metrics may provide additional objective information to clinical tests by quantifying the amount of UL movements and the amount of asymmetry between the upper limbs in daily life. Accelerometer-based metrics of the upper limbs offer additional information about the quantity of upper limb asymmetry and relative use in children and adolescents with unilateral Cerebral Palsy.Accelerometer-based metrics of upper limb asymmetry in sedentary time and different physical activity intensities in daily life can be a complement to clinical tests among children and adolescents with unilateral Cerebral Palsy. Accelerometer-based metrics of the upper limbs offer additional information about the quantity of upper limb asymmetry and relative use in children and adolescents with unilateral Cerebral Palsy. Accelerometer-based metrics of upper limb asymmetry in sedentary time and different physical activity intensities in daily life can be a complement to clinical tests among children and adolescents with unilateral Cerebral Palsy.