cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12127260.v1
title: In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

description: ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.
DOI: 10.6084/m9.figshare.12127248
title: Agronomic potential of BC1F2 dwarf round tomato populations

description: ABSTRACT The use of dwarf lines to obtain mini-tomato hybrids has provided agronomic and economic benefits. In Brazil, round tomatoes predominate over other varieties. The benefits of using a dwarf parent in round tomato hybrids has yet to be explored, making it important to develop dwarf round tomato lines. Backcrossing is the most suitable method to develop these lines. Evaluation and selection of the dwarf populations can improve the development of such lines. Thus, the aim of this study was to select BC1F2 populations of dwarf round tomatoes with agronomic potential and high-quality fruit. The study was conducted at the Vegetable Experimental Station of the Federal University of Uberlândia (UFU). A randomized block design was used, with 15 treatments and three replicates. The genetic material analyzed consisted of 12 BC1F2 dwarf tomato populations, plus both parents (recurrent and donor) and a commercial hybrid. The characteristics assessed were: average fruit weight (g), total soluble solids (ºBrix), number of locules (locules per fruit-1), fruit shape, pulp thickness (cm), longitudinal (cm) and transverse fruit diameter (cm), internode length (cm) and plant height (cm). The data were submitted to mean testing, multivariate analyses and a selection index. In general, average fruit weight in the dwarf populations increased significantly after the first backcross, with some fruits exhibiting a similar shape to round tomatoes. Selection of the populations UFU-DTOM7, UFU-DTOM10, UFU-DTOM5, UFU-DTOM9, and UFU-DTOM3 resulted in an estimated 6% increase in the number of locules, transverse diameter, TD/LD ratio and average fruit weight. The BC1F2 dwarf populations UFU-DTOM7 and UFU-DTOM10 were the most promising for develop inbred lines with round fruits. Despite the considerable progress achieved in this study, we suggest a second backcross, in order to obtain lines and, posteriorly, hybrids with round fruits and compact plants.
DOI: 10.6084/m9.figshare.12127254.v1
title: Nutritional status and physiological parameters of maize cultivated with sewage sludge

description: ABSTRACT The use of sewage sludge as a source of nutrients and organic matter for agricultural soils is a well-established practice. However, few reports highlight the effect of the nutrients and potentially toxic elements provided by organic wastes application on the plant physiological parameters, such as photosynthetic activity and stomatal conductivity. We performed a greenhouse experiment with maize exposed to a dystrophic red Latosol amended with mineral fertilizer and different rates of sewage sludge with the following objectives: i) assess the nutrients and metal uptake translocation and distribution in plants and ii) evaluate the relationship between plant physiological parameters and yield indicators under the study conditions. The application of sewage sludge increased the soil organic matter, pH, and the amounts of available Ca, S, and Mg, comparing to the mineral fertilizer treatment. The plants promote a higher translocation of macronutrients to the shoots in the sewage sludge treatments, which results in higher photosynthetic activity, stomatal conductivity, and maize yield parameters. Moreover, the trace elements, which can cause toxicity in small concentrations, were founded mainly in the roots, which indicates a plant defense mechanism.
DOI: 10.6084/m9.figshare.12127239
title: Spatial diffusion of Zika fever epidemics in the Municipality of Salvador-Bahia, Brazil, in 2015-2016: does Zika fever have the same spread pattern as Dengue and Chikungunya fever epidemics?

description: Abstract INTRODUCTION The recent emergence and rapid spread of Zika and Chikungunya fevers in Brazil, occurring simultaneously to a Dengue fever epidemic, together represent major challenges to public health authorities. This study aimed to identify and compare the 2015-2016 spatial diffusion pattern of Zika, Chikungunya, and Dengue epidemics in Salvador-Bahia. METHODS We used two study designs comprising a cross-sectional-to-point pattern and an ecological analysis of lattice data. Residential addresses involving notified cases were geocoded. We used four spatial diffusion analysis techniques: (i) visual inspection of the sequential kernel and choropleth map, (ii) spatial correlogram analysis, (iii) spatial local autocorrelation (LISA) changes analysis and, (iv) nearest neighbor index (NNI) modeling. RESULTS Kernel and choropleth maps indicated that arboviruses spread to neighboring areas near the first reported cases and occupied these new areas, suggesting a diffusion expansion pattern. A greater case density occurred in central and western areas. In 2015 and 2016, the NNI best-fit model had an S-curve compatible with an expansion pattern for Zika (R2 = 0.94; 0.95), Chikungunya (R2 = 0.99; 0.98) and Dengue (R2 = 0.93; 0.99) epidemics, respectively. Spatial correlograms indicated a decline in spatial lag autocorrelations for the three diseases (expansion pattern). Significant LISA changes suggested different diffusion patterns, although a small number of changes were detected. CONCLUSIONS These findings indicate diffusion expansion, a unique spatial diffusion pattern of Zika, Chikungunya, and Dengue epidemics in Salvador-Bahia, namely. Knowing how and where arboviruses spread in Salvador-Bahia can help improve subsequent specific epidemic control interventions.
DOI: 10.6084/m9.figshare.12127242
title: Nutrition, yield and nutrient export in common bean under zinc fertilization in no-till system

description: ABSTRACT Zinc (Zn) is one of the most deficient plant micronutrients in agricultural crops. The objective of this study was to evaluate plant nutrition, grain yield and nutrient export rate in response to soil and foliar Zn fertilization in common bean (Phaseolus vulgaris L.). Two field experiments in no-till system were carried out using two common bean cultivars, BRS Esteio (black bean) and IPR Campos Gerais (Carioca bean). Treatments were composed of soil Zn application during sowing and foliar Zn spray at flowering stage. Soil Zn application had effect on leaf Zn concentration in IPR Campos Gerais and did not affect grain yield of both cultivars. Foliar Zn spray increased leaf Zn concentration by approximately two times in both cultivars, but negatively affected the grain yield in BRS Esteio. Leaf concentration of N, Ca and S were affected by soil Zn application and leaf concentration of Mn was affected by foliar Zn spray, while leaf concentration of P, K, Mg, Cu and Fe were not influenced by the soil and foliar Zn treatments. In treatments without Zn, the descending order of nutrient export rate from the experimental site was as follows: N &gt; K &gt; P &gt; Ca ≈ S &gt; Mg for macronutrients and Fe &gt; Mn &gt; Cu &gt; Zn for micronutrients. Foliar Zn spray increased the export rate of Zn, P, Ca, Mg, S, Mn, Cu and Fe in IPR Campos Gerais, while soil Zn application resulted in higher export rate of P, K and Mn in BRS Esteio.
DOI: 10.6084/m9.figshare.12127257.v1
title: Thermodynamic properties of moisture desorption isotherms of ryegrass (Lolium multiflorum L.) seeds

description: ABSTRACT Studies about the thermodynamic properties of ryegrass seeds are necessary to improve post-harvest processes, relating the factors that affect product quality with the interaction between water and its chemical components. Given the importance of recognizing and understanding the intrinsic behavior of water in ryegrass seeds and providing data for the improvement of industrial drying equipment, this work aimed to calculate and evaluate the thermodynamic properties of moisture desorption of ryegrass seeds as a function of the equilibrium moisture content. Ryegrass seeds with initial moisture content of 10.4 (% d.b.) was used. The equilibrium moisture content of seeds was determined by static-gravimetric method at different temperatures (10, 20, 30, 40, and 50 °C) and water activity values (between 0.10 and 0.90), in three repetitions. The Chung Pfost model presented the best fit to the experimental data. It was observed that the integral isosteric desorption heat increased as the equilibrium moisture content decreased, ranged from 2499.95 to 4241.96 kJ kg-1 in the moisture content range 2.80 to 22.10 (% d.b.). Differential entropy also increased with decreasing equilibrium moisture content, as did Gibbs free energy, being positive for all temperature studied, indicating that ryegrass seeds desorption is a non-spontaneous process. The enthalpy-entropy compensation theory was satisfactorily applied to the sorption phenomenon, being controlled by enthalpy.
DOI: 10.6084/m9.figshare.12127233
title: Characterization of cattle tick fever in calves from the northwestern region of Minas Gerais, Brazil

description: Abstract The present study aimed to characterize the importance of the Anaplasma marginale, Babesia bovis and Babesia bigemina in the genesis of cattle tick fever (CTF) among dairy calves in the northwest of Minas Gerais, Brazil. Blood samples from 300 calves were collected, followed by DNA extraction and nested PCR using oligonucleotide primers to amplify fragments of the semi-nested for the msp5 gene (A. marginale), sbp-4 (B. bovis) and rap-1a (B. bigemina) Among the examined calves, the prevalence of A. marginale was 55.6% (n=167/300), B. bovis was 4.0% (n=12/300) and B. bigemina was 15.3% (n=46/300), by PCR techniques. Parasitic forms of A. marginale and B. bigemina were found in 36,3% and 2,6% of the blood smears while B. bovis was not detected. There was a statistical difference between the positivity of infected animals in the age groups 1 (10-70 days) and (&gt;70-300 days) for A. marginale and B. bigemina. A total of 15 calves with the classic symptoms of disease were examined, and the samples obtained were confirmed as a simple infection by A. marginale through semi-nested PCR. These results confirm bovine anaplasmosis as the primary cause of CTF among the calves of dairy cattle within the studied area.
DOI: 10.6084/m9.figshare.12127239.v1
title: Spatial diffusion of Zika fever epidemics in the Municipality of Salvador-Bahia, Brazil, in 2015-2016: does Zika fever have the same spread pattern as Dengue and Chikungunya fever epidemics?

description: Abstract INTRODUCTION The recent emergence and rapid spread of Zika and Chikungunya fevers in Brazil, occurring simultaneously to a Dengue fever epidemic, together represent major challenges to public health authorities. This study aimed to identify and compare the 2015-2016 spatial diffusion pattern of Zika, Chikungunya, and Dengue epidemics in Salvador-Bahia. METHODS We used two study designs comprising a cross-sectional-to-point pattern and an ecological analysis of lattice data. Residential addresses involving notified cases were geocoded. We used four spatial diffusion analysis techniques: (i) visual inspection of the sequential kernel and choropleth map, (ii) spatial correlogram analysis, (iii) spatial local autocorrelation (LISA) changes analysis and, (iv) nearest neighbor index (NNI) modeling. RESULTS Kernel and choropleth maps indicated that arboviruses spread to neighboring areas near the first reported cases and occupied these new areas, suggesting a diffusion expansion pattern. A greater case density occurred in central and western areas. In 2015 and 2016, the NNI best-fit model had an S-curve compatible with an expansion pattern for Zika (R2 = 0.94; 0.95), Chikungunya (R2 = 0.99; 0.98) and Dengue (R2 = 0.93; 0.99) epidemics, respectively. Spatial correlograms indicated a decline in spatial lag autocorrelations for the three diseases (expansion pattern). Significant LISA changes suggested different diffusion patterns, although a small number of changes were detected. CONCLUSIONS These findings indicate diffusion expansion, a unique spatial diffusion pattern of Zika, Chikungunya, and Dengue epidemics in Salvador-Bahia, namely. Knowing how and where arboviruses spread in Salvador-Bahia can help improve subsequent specific epidemic control interventions.
DOI: 10.6084/m9.figshare.12127242.v1
title: Nutrition, yield and nutrient export in common bean under zinc fertilization in no-till system

description: ABSTRACT Zinc (Zn) is one of the most deficient plant micronutrients in agricultural crops. The objective of this study was to evaluate plant nutrition, grain yield and nutrient export rate in response to soil and foliar Zn fertilization in common bean (Phaseolus vulgaris L.). Two field experiments in no-till system were carried out using two common bean cultivars, BRS Esteio (black bean) and IPR Campos Gerais (Carioca bean). Treatments were composed of soil Zn application during sowing and foliar Zn spray at flowering stage. Soil Zn application had effect on leaf Zn concentration in IPR Campos Gerais and did not affect grain yield of both cultivars. Foliar Zn spray increased leaf Zn concentration by approximately two times in both cultivars, but negatively affected the grain yield in BRS Esteio. Leaf concentration of N, Ca and S were affected by soil Zn application and leaf concentration of Mn was affected by foliar Zn spray, while leaf concentration of P, K, Mg, Cu and Fe were not influenced by the soil and foliar Zn treatments. In treatments without Zn, the descending order of nutrient export rate from the experimental site was as follows: N &gt; K &gt; P &gt; Ca ≈ S &gt; Mg for macronutrients and Fe &gt; Mn &gt; Cu &gt; Zn for micronutrients. Foliar Zn spray increased the export rate of Zn, P, Ca, Mg, S, Mn, Cu and Fe in IPR Campos Gerais, while soil Zn application resulted in higher export rate of P, K and Mn in BRS Esteio.
DOI: 10.6084/m9.figshare.12127248.v1
title: Agronomic potential of BC1F2 dwarf round tomato populations

description: ABSTRACT The use of dwarf lines to obtain mini-tomato hybrids has provided agronomic and economic benefits. In Brazil, round tomatoes predominate over other varieties. The benefits of using a dwarf parent in round tomato hybrids has yet to be explored, making it important to develop dwarf round tomato lines. Backcrossing is the most suitable method to develop these lines. Evaluation and selection of the dwarf populations can improve the development of such lines. Thus, the aim of this study was to select BC1F2 populations of dwarf round tomatoes with agronomic potential and high-quality fruit. The study was conducted at the Vegetable Experimental Station of the Federal University of Uberlândia (UFU). A randomized block design was used, with 15 treatments and three replicates. The genetic material analyzed consisted of 12 BC1F2 dwarf tomato populations, plus both parents (recurrent and donor) and a commercial hybrid. The characteristics assessed were: average fruit weight (g), total soluble solids (ºBrix), number of locules (locules per fruit-1), fruit shape, pulp thickness (cm), longitudinal (cm) and transverse fruit diameter (cm), internode length (cm) and plant height (cm). The data were submitted to mean testing, multivariate analyses and a selection index. In general, average fruit weight in the dwarf populations increased significantly after the first backcross, with some fruits exhibiting a similar shape to round tomatoes. Selection of the populations UFU-DTOM7, UFU-DTOM10, UFU-DTOM5, UFU-DTOM9, and UFU-DTOM3 resulted in an estimated 6% increase in the number of locules, transverse diameter, TD/LD ratio and average fruit weight. The BC1F2 dwarf populations UFU-DTOM7 and UFU-DTOM10 were the most promising for develop inbred lines with round fruits. Despite the considerable progress achieved in this study, we suggest a second backcross, in order to obtain lines and, posteriorly, hybrids with round fruits and compact plants.
DOI: 10.6084/m9.figshare.12127224
title: Post-dural puncture headache incidence after cerebrospinal fluid aspiration. A prospective observational study

description: Abstract Background: Post-dural puncture headache (PDPH) is an iatrogenic condition following lumbar puncture (LP). Incidence is variable and often associated with young females. Technical features of the procedure (i.e. needle gauge) have been investigated; however there is no investigation on the method of cerebrospinal fluid (CSF) collection. Objective: To investigate whether mild CSF aspiration is associated with increased PDPH in selected patients. Methods: 336 subjects were eligible to the study. Data on 237 patients from a tertiary neurology hospital who underwent diagnostic LP from February 2010 to December 2012 were analysed. Patient demographics, lumbar puncture method, CSF biochemical characteristics, opening pressures, and a follow-up inquire on PDPH occurrence were collected. CSF was collected either by allowing free flow or by mild aspiration. Results: The aspiration arm (n=163) was comprised of 55.8% females with mean age of 52(35‒69) years. Sex distribution was not different between the two arms (p=0.191). A significant larger amount of CSF was obtained in the aspiration arm (p=0.011). The incidence of PDPH in the aspiration arm was 16.5% versus 20.2% in the free flow arm, not statistically significant (p=0.489). No relevant associations emerged from the analyses in the subgroup aged &lt;65 years. Conclusions: Aspiration of the CSF during LP was not associated with increased rates of PDPH compared to the standard method, particularly when larger amounts of CSF are required and ideal conditions are met. This is the first study looking into this matter, aiming to add safety to the procedure. Further randomized trials are required.
DOI: 10.6084/m9.figshare.12127227
title: Longitudinal study of the sympathovagal balance in women submitted to bariatric surgery

description: Abstract Studies have shown improvement of the cardiac autonomic balance in post-bariatric patients. Most of these studies included patients using drugs interfering in the autonomic nervous system. This study assessed the time course of changes in the sympathovagal balance after bariatric surgery (Roux-en-Y gastric bypass) in 26 women free from drugs. A 10-min electrocardiographic recording was obtained at baseline and at 3 and 6 months after surgery. Temporal and spectral domains of heart rate variability were analysed. The time domain components of cardiac vagal modulation increased progressively after surgery. In the frequency domain, high frequency power increased from 24.9 at baseline (18.0 to 46.3) to 44.5 at 3 months (23.4 to 65.6), and 54.1 at 6 months after surgery (37.6 to 64.0) (median and IQR in normalized units). Low frequency spectral power decreased from 56.2 at baseline (39.6 to 74.4) to 36.8 at 6 months after surgery (24.9 to 53.9) (P= 0.036). Low frequency/high frequency ratio decreased from 2.3 at baseline (1.0 to 4.2) to 0.8 at 6 months after surgery (0.4 to 1.3) (P= 0.038). Progressive shift towards predominance of vagal tonus was detected in the follow-up. Most of the patients recovered low frequency/high frequency at 6 months after surgery.
DOI: 10.6084/m9.figshare.12127230
title: Estimated mortality rate and leading causes of death among individuals with chikungunya in 2016 and 2017 in Brazil

description: Abstract INTRODUCTION: In 2014, the first cases of autochthonous chikungunya (CHIK) were recorded in Brazil. Lethality associated with this disease is underestimated. Thus, this study aimed to analyze the causes of death among individuals with CHIK in Brazil. METHODS: A descriptive observational study was conducted on individuals with CHIK who died within 6 months from symptom onset. Data pairing between the Information System for Notifiable Diseases and the Mortality Information System was performed. Deaths were classified according to case confirmation criterion, mention of CHIK in the death certificates (DCs), and disease phase. The lethality rate per 1,000 cases was corrected for underreporting and was estimated according to region, sex, age, years of education, race/color, and cause groups. RESULTS: We identified 3,135 deaths (mention of CHIK in the DCs, 764 [24.4%]). In 17.6% of these cases, CHIK was the underlying cause. Most deaths occurred in the acute (38.1%) and post-acute (29.6%) phases. The corrected LR (5.7; x1,000) was 6.8 times higher than that obtained from the Information System for Notifiable Diseases (0.8). The highest corrected LRs were estimated for among individuals living in the Northeast region (6.2), men (7.4), those with low years of education and those aged &lt;1 year (8.6), 65-79 years (20.7), and ≥80 years (75.4). CONCLUSIONS: The LR of CHIK estimates based on information system linkage help to reveal the relevance of this disease as the direct cause or as a cause associated with serious or fatal events, provide timely interventions, and increase the knowledge about this disease.
DOI: 10.6084/m9.figshare.12127233.v1
title: Characterization of cattle tick fever in calves from the northwestern region of Minas Gerais, Brazil

description: Abstract The present study aimed to characterize the importance of the Anaplasma marginale, Babesia bovis and Babesia bigemina in the genesis of cattle tick fever (CTF) among dairy calves in the northwest of Minas Gerais, Brazil. Blood samples from 300 calves were collected, followed by DNA extraction and nested PCR using oligonucleotide primers to amplify fragments of the semi-nested for the msp5 gene (A. marginale), sbp-4 (B. bovis) and rap-1a (B. bigemina) Among the examined calves, the prevalence of A. marginale was 55.6% (n=167/300), B. bovis was 4.0% (n=12/300) and B. bigemina was 15.3% (n=46/300), by PCR techniques. Parasitic forms of A. marginale and B. bigemina were found in 36,3% and 2,6% of the blood smears while B. bovis was not detected. There was a statistical difference between the positivity of infected animals in the age groups 1 (10-70 days) and (&gt;70-300 days) for A. marginale and B. bigemina. A total of 15 calves with the classic symptoms of disease were examined, and the samples obtained were confirmed as a simple infection by A. marginale through semi-nested PCR. These results confirm bovine anaplasmosis as the primary cause of CTF among the calves of dairy cattle within the studied area.