DOI: 10.5061/dryad.2v6wwpzvq
title: Multilocus microsatellite genotypes and population geographical coordinates in the southern Damselfly (Coenagrion mercuriale)

description: Aim Accelerating environmental changes caused by human activities result
 in habitat loss and fragmentation, profoundly affecting wildlife
 population genetic structure and evolution. Urbanised and geographically
 marginal areas are often characterized by unfavourable environments,
 reducing the connectivity among populations and resulting in an increase
 in population genetic differentiation and a decrease in intra-population
 genetic diversity. In this context, we examined the effect of geographic
 marginality and anthropogenic pressures on population genetic features in
 the protected southern damselfly (Coenagrion mercuriale, Odonata), which
 has low dispersal capabilities and specific habitat requirements and whose
 populations are declining. Location Two contrasting areas were studied:
 one located in semi-natural habitats at the northern limit of the species
 geographic range (northern France) and the second one located within the
 species’ range core, in an urbanised area surrounding the city of
 Strasbourg (Alsace, eastern France). Methods We genotyped 2743 individuals
 from 128 populations using microsatellite loci, and analysed the spatial
 distribution of neutral genetic diversity and the levels of genetic
 differentiation within the two areas. We also examined fine-scale patterns
 of genetic structure and migration rate in the urbanised area of Alsace.
 Results Northern peripheral populations showed lower levels of genetic
 diversity and higher levels of genetic differentiation compared to central
 Alsacian populations. Although located in anthropized habitats,
 geographically central Alsacian populations exhibited substantial levels
 of gene flow, with dispersal events mainly occurring overland and not
 restricted to watercourses. However, the highly urbanised city of
 Strasbourg negatively impacted the neighbouring populations by decreasing
 levels of genetic diversity and migration rates, thereby magnifying the
 extent of genetic differentiation. Main conclusions Altogether, these
 results suggested the need for management action by restoring breeding
 sites and creating migratory corridors for marginal southern damselfly
 populations. However, our results also highlighted the resilience of
 southern damselfly in central range populations even facing strong
 urbanisation pressures.
description: Genotyping of individuals using microsatellite loci.
description: # Southern\_damselfly\_2023\_Sample\_genotype\_and\_locations
 [https://doi.org/10.5061/dryad.2v6wwpzvq](https://doi.org/10.5061/dryad.2v6wwpzvq) **Contrasting patterns of spatial genetic structure in endangered southern damselfly (Coenagrion mercuriale) populations facing habitat fragmentation and urbanisation. Diversity and Distributions** [Submitted manuscript] **Data S1** Samples raw data (genotype and locations) **File list** Southern_Damselfly_2023_Sample_genotype_and_locations.xlsx **File descriptions** Southern_damselfly_2023_Sample_genotype_and_locations.xlsx – File containing for each sample: * the name of the individual (ID_Sample), * the name of the site where it was sampled (ID_Site), * the population to which it belongs (ID_Population), * the sampling year (Year), * the region where it was sampled (Region), * its location (xcoord_WGS84. EPSG4326, ycoord_WGS84.EPSG4326), * its genotype at the 11 microsattelite loci (l24,l34,l35,l63,l66,l02,l23,l37,l42,l60,l62).