DOI: 10.5061/dryad.xpnvx0k9x
title: Data from: The contribution of hybridization to range‐wide population genetic structure in a Pacific coastal dune plant

description: Premise of the study: Interspecific hybridization can cause genetic
 structure across species ranges if the mating system and degree of
 sympatry/parapatry with close relatives varies geographically. The coastal
 dune endemic Camissoniopsis cheiranthifolia (Onagraceae) exhibits genetic
 subdivisions across its range, some of which are associated with shifts in
 mating system from outcrossing to selfing, while others are not. For
 instance, strong differentiation between large-flowered, self-incompatible
 (LF-SI) and large-flowered, self-compatible (LF-SC) populations occurs
 without much reduction in outcrossing or obvious barriers to gene flow. We
 hypothesized that LF-SI diverged from LF-SC via hybridization with the
 predominantly inland SI sister species C. bistorta. Methods: We analyzed
 spatial proximity using 1460 herbarium records, and genetic variation at
 12 microsatellites assayed for 805 and 404 individuals from 32 C.
 cheiranthifolia and 18 C. bistorta populations, respectively. We also
 assayed nine chloroplast microsatellites for 124 and 111 individuals from
 27 and 19 populations, respectively.  Key results: Closer
 parapatry was associated with unexpectedly high genetic continuity between
 LF-SI C. cheiranthifolia and C. bistorta. LF-SI genotypes clustered with
 C. bistorta exclusive of other C. cheiranthifolia genotypes. Similarly,
 pairwise FST among SI C. cheiranthifolia and C. bistorta, adjusted for
 geographic proximity, was not higher between heterospecific than
 conspecific populations.  Conclusions: The lack of genetic
 differentiation between LF-SI C. cheiranthifolia and C. bistorta
 populations, even those located away from the zone of parapatry, suggests
 that LF-SI C. cheiranthifolia instead of hybridizing with C. bistorta is
 rather an ecotype of C. bistorta that has adapted to coastal dune habitat
 independent of other lineages in C. cheiranthifolia proper.
description: The nuclear microsatellite (nSSR) dataset represents 129
 genotypes at 12 loci from 50 populations across the range of
 <em>Camissoniopsis cheiranthifolia</em> (32 populations) and
 <em>C. bistorta </em>(18 populations) collected in 2009 and
 2010. Fragment sizes were binned using the MsatAllele package (version
 1.04, Alberto 2009) for the R statistical computing environmt.For details
 about PCR conditions and other laboratory methods
 please see López-Villalobos et al., 2014. We provide
 individual and population codes, latitude, longitude, location (coastal
 vs. inland), species (C.ch = <em>C. cheiranthifolia</em>, C.bi
 = <em>C. bistorta</em>) and the categorical variable used to
 identify gentic clusters and mating system variation (please, refer to the
 paper). Loci names are as presented in the supplementary information of
 this study and in López-Villalobos et al., 2014 and López-Villalobos and
 Eckert 2018. The chloroplast microsatellite data
 represents number of variants (base pair repeats)  at nine polymorphic
 chloroplast DNA microsatellites (cpSSR; following Weising and Gardner,
 1999; Chung and Staub, 2003) assyed for 124 individuals from 27
 populations of  <em>C. cheiranthifolia </em>and 111
 <em>C. bistorta</em> individuals from  19
 populations. The third dataset are pairwise
 F<sub>ST</sub> [Weir and Cockerham,1984] and other genetic
 differentiation statistics from nSSR data estimated using the R package
 DiveRsity.This data was obtained using the methods described in the
 isolation-by-distance analyses from this study. Metadata for each colum is
 as follows: population comparison (codes as in nSSR dataset), population
 1, population 2, latitude and longitude of populations 1 and 2, grpspp
 (species grouping, wCc = within <em>C. cheiranthifolia</em>,
 wCbi = within <em>C. bistorta</em>, bSp = between species),
 group according to whether populations are coastal (Co) or inland (In),
 gouping according to their genetic cluster as in Figure 5 (P=pink,
 B=Blue), geographic distance estimated as the great circle surface
 distance calculated using the R package geosphere, version 1.3-11 and
 several genetic differentiation statistics (Fst used in this
 paper) estimated using the R package DiveRsity.