DOI: 10.5061/dryad.bvq83bkpz
title: Data from: The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs

description: Drug hypersensitivity reactions (DHR) are commonly observed with various
 medications. However, some drugs, like Qingkailing (QKL), iodixanol, and
 vancomycin, previously classified as pseudoallergenic, do not align with
 clinical observations, creating uncertainty in understanding DHR
 mechanisms and implementing prevention. In response, our research team
 hypothesized that IgE-mediated allergy and pseudoallergic hypersensitivity
 may synergistically lead to more severe reactions. We established an
 IgE-mediated hypersensitivity model using an anti-dinitrophenyl IgE
 monoclonal antibody in RBL-2H3 cells and employed ovalbumen (OVA) for
 guinea pigs. Compound 48/80 served as a positive control for
 non-immunologic hypersensitivity. We measured β-hexosaminidase and
 histamine released in cells and guinea pigs to focus on hypersensitivity
 responses from both IgE-mediated and pseudoallergic pathways. Results
 indicated that combining pseudoallergenic drugs and IgE significantly
 increased the hypersensitivity response compared to IgE or separate
 compound 48/80 groups. This effect was mirrored in the OVA-induced model.
 Notably, the three pseudoallergic drugs exhibited a similar increase in
 IgE-mediated hypersensitivity. These findings highlight the additive
 effects of IgE-mediated and pseudoallergic hypersensitivity, suggesting a
 more potent DHR response. The study also measured several cytokines and
 developed a method to differentiate between allergic, pseudoallergic DHRs,
 and additive reactions, aiding in understanding and controlling serious
 DHR risks in clinical practice.
description: # Data from: The additive effect of IgE-mediated and pseudoallergic
 hypersensitivity in RBL-2H3 cells and guinea pigs Dataset DOI:
 [10.5061/dryad.bvq83bkpz](https://doi.org/10.5061/dryad.bvq83bkpz) ##
 Description of the data and file structure This dataset supports the
 research article titled "The additive effect of IgE-mediated and
 pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs"
 (PLOS ONE Manuscript ID: PONE-D-25-28449R2), authored by Yu Zhang, Chengbo
 Zheng, Qilong Xu, Cunyu Li, Yunfeng Zheng, and Guoping Peng. ### Files and
 variables #### File: DATA_PONED2528449R2_YuZhang_20260124.xlsx
 **Description:** In vitro experiments were conducted using RBL-2H3 cells,
 a widely used model for studying allergic responses. The dataset includes
 key measurements such as cell degranulation rates (assessed by
 β-hexosaminidase release assay), levels of inflammatory cytokines (e.g.,
 IL-4, TNF-α) detected via ELISA, and cell viability data. These data
 reflect the response of RBL-2H3 cells to stimuli related to IgE-mediated
 and pseudoallergic pathways, as well as their combined effects. In vitro
 experiments were conducted using RBL-2H3 cells, a widely used model for
 studying allergic responses. The dataset includes key measurements such as
 cell degranulation rates (assessed by β-hexosaminidase release assay),
 levels of inflammatory cytokines (e.g., IL-4, TNF-α) detected via ELISA,
 and cell viability data. These data reflect the response of RBL-2H3 cells
 to stimuli related to IgE-mediated and pseudoallergic pathways, as well as
 their combined effects. In vivo experiments were performed on guinea pigs,
 which were divided into appropriate groups based on experimental design.
 The dataset contains physiological and biochemical data from guinea pigs,
 including changes in body temperature, skin reaction scores (assessing
 allergic edema and erythema), serum levels of histamine and specific IgE,
 and pathological observations of relevant tissues. These data provide
 direct evidence for the additive effect of the two hypersensitivity types
 in a mammalian model. All data have been de-identified and processed in
 compliance with relevant ethical and scientific standards. Detailed
 experimental protocols, including cell culture conditions, stimulus
 concentrations, animal handling procedures, and detection methods, are
 described in the accompanying research article. This dataset is essential
 for replicating the study's findings and can serve as a valuable
 resource for other researchers investigating allergic and pseudoallergic
 mechanisms, as well as their interactions. This dataset contains the
 source data for the figures included in the main article, which were
 generated from both in vitro and in vivo experiments designed to
 investigate the additive effects of IgE-mediated and pseudoallergic
 hypersensitivity. ##### Variables * Control/Untreated cells/animals
 (negative control) * IgE, Cells treated with IgE + DNP-BSA (positive
 control for allergic activation) * C48/80, Cells treated with compound
 48/80 (positive control for pseudoallergic activation) * OVA, Guinea pigs
 sensitized with ovalbumin (OVA) * QKL/Iodi/Van-treatedCells/animals
 treated with test compounds (Qingkailing Injection, \ Iodixanol,
 vancomycin) * β-Hex, β-HexosaminidaseLysosomal enzyme,relative rate% *
 IgE, Immunoglobulin E,, (ng/ml) * His, Histamine, relative rate% *
 IL-4,Interleukin4,(pg/ml) * IL-13,Interleukin13, (pg/ml) * IL-6,
 Interleukin6, (ng/ml) * C3a, Complement Component 3, (ng/ml) *
 C5a, Complement Component 5, (ng/ml) * SC5b-9, Terminal Complement
 Complex, TCC, (ng/ml) ## Code/software The data is stored in Microsoft
 Excel format (.xlsx) containing 14 worksheets corresponding to different
 figures (Fig1-Fig10, S1-S6 Fig). **Free/open software for viewing:** -
 LibreOffice Calc (version 7.0 or higher) - Apache OpenOffice Calc (version
 4.1 or higher) - Google Sheets (latest online version) **For advanced
 analysis:** - Python 3.8+ with packages: pandas (1.0+), openpyxl (3.0+),
 matplotlib/seaborn - Workflow: Read specific sheets using
 pandas.read_excel(), perform data cleaning/analysis, and reproduce figures
 with visualization libraries. No additional code or scripts are required
 for basic data viewing. ## Access information Other publicly accessible
 locations of the data: * This dataset is only publicly accessible via
 Dryad, with no additional copies hosted on other public databases. Data
 was derived from the following sources: * This dataset is original raw
 data generated directly by the authors through laboratory experiments. No
 part of this data was derived from pre-existing public datasets or other
 external sources.