DOI: 10.5061/dryad.25b39
title: Data from: Temporal variation of Bistorta vivipara-associated ectomycorrhizal fungal communities in the High Arctic

description: Ectomycorrhizal (ECM) fungi are important for efficient nutrient uptake of
 several widespread arctic plant species. Knowledge of temporal variation
 of ECM fungi, and the relationship of these patterns to environmental
 variables, is essential to understand energy and nutrient cycling in
 Arctic ecosystems. We sampled roots of Bistorta vivipara ten times over
 two years; three times during the growing-season (June, July and
 September) and twice during winter (November and April) of both years. We
 found 668 ECM OTUs belonging to 25 different ECM lineages, whereof 157
 OTUs persisted throughout all sampling time-points. Overall, ECM fungal
 richness peaked in winter and species belonging to Cortinarius,
 Serendipita and Sebacina were more frequent in winter than during summer.
 Structure of ECM fungal communities was primarily affected by spatial
 factors. However, after accounting for spatial effects, significant
 seasonal variation was evident revealing correspondence with seasonal
 changes in environmental conditions. We demonstrate that arctic ECM
 richness and community structure differ between summer (growing-season)
 and winter, possibly due to reduced activity of the core community, and
 addition of fungi adapted for winter conditions forming a winter-active
 fungal community. Significant month × year interactions were observed both
 for fungal richness and community composition, indicating unpredictable
 between-year variation. Our study indicates that addressing seasonal
 changes requires replication over several years.
description: OTU matrix total fungi
 Abu and PA dataThis file contains all
 the fungal OTUs recovered, including ECM and non ECM, abundance as well as
 presence-absence dataOTU matrix ECM fungi Abu
 and PA dataThis file contains all
 the ECM fungal OTUs recovered, abundance as well as presence-absence
 dataEnvironmental data
 matirx (raw data)This file contains
 information about raw environmental data associated with each
 sample.Shared species analysis
 EstimateSOTU Data for shared
 analysis performed using EstimateSShared species
 analysisR script for plotting
 shared species between sampling monthsGNMDs ordination
 analysisR script for GNMDS
 analysis of ECM fungiIllumina sequencing
 plate1This file contains both
 forward (t1-UNIS_S1_L001_R1_001.fastq) and reverse
 (t1-UNIS_S1_L001_R2_001.fastq) Illumina read data and associated barcode
 mapping files. In Mapfile_T1 forward primer is linker primer and in
 Mapfile_T1_rev reverse primer is the linker primer to recover all the
 reads from mix orientation read data files. which is the case in our
 sequencing.Illumina_sequencing_plate1.zipIllumina sequencing plate2This file contains both forward (t2-UNIS_S1_L001_R1_001.fastq) and reverse (t2-UNIS_S1_L001_R2_001.fastq) Illumina read data and associated barcode mapping files. In Mapfile_T2 forward primer is linker primer and in Mapfile_T2_rev reverse primer is the linker primer to recover all the reads from mix orientation read data files. which is the case in our sequencing.Illumina_sequencing_plate2.zip