DOI: 10.6084/m9.figshare.1177910.v1
title: Raw data of qRT-PCR and western blot analyses of proteasome subunits and GFP-CL1 degradation in Podospora anserina

description: Dataset 1 : Raw data of qRT-PCR analysis of the PaPre3 gene used in Figure 1A<br>CP values of the reference gene PaPorin and of the target gene PaPre3 are displayed for juvenile middle-aged and senescent samples Dataset 2 : Raw data of qRT-PCR analysis of the PaPre2 gene used in Figure 1A<br>CP values of the reference gene PaPorin and of the target gene PaPre2 are displayed for juvenile middle-aged and senescent samples Dataset 3 : Raw data of qRT-PCR analysis of the PaUmp1 gene used in Figure 1A<br>CP values of the reference gene PaPorin and of the target gene PaUmp1 are displayed for juvenile, middle-aged and senescent samples Dataset 4 : Raw data of qRT-PCR analysis of the PaPre3 gene used in Figure 2A<br>CP values of the reference gene PaPorin and of the target gene PaPre3 are displayed. The wild type CP is the mean CP value of juvenile samples displayed in Dataset 1 Dataset 5 : Raw data of qRT-PCR analysis of the PaPre2 gene used in Figure 2B<br>CP values of the reference gene PaPorin and of the target gene PaPre2 are displayed. The wild type CP is the mean CP value of the juvenile samples displayed in Dataset 2 Dataset 6: Raw data of western blot displayed in Figure 1B probed with α-PaPRE2. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE2. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM (Cat# 26616) Prestained protein ladder. Lanes 2 – 7: Samples described in Figure 1B. Dataset 7: Raw data of western blot displayed in Figure 1B probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 7 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 1 – 6: Samples described in Figure 1B. Dataset 8: Raw data of western blot displayed in Figure 1B probed with α-PaPUP1. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPUP1. Red signal represents fluorescence at 700 nm. Lane 10 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 1 – 6: Samples described in Figure 1B. Lanes 7 - 9 are not relevant to this study. Dataset 9: Raw data of western blot displayed in Figure 1B probed with α-PaHSP60. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-PaHSP60. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 2 - 7: Samples described in Figure 1B. Dataset 10: Raw data of western blot displayed in Figure 2C probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 – 5 and lane 9: Samples described in Figure 1C. The other lanes are not relevant to this study. Dataset 11: Raw data of western blot displayed in Figure 2D probed with α-PaPRE2. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE2. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 and 4: Samples described in Figure 1D. Lane 2 is not relevant to this study. Dataset 12: Raw data of western blot displayed in Figure 2D probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 and 4: Samples described in Figure 1D. Lane 2 is not relevant to this study. Dataset 13: Raw data of western blot displayed in Figure 3B probed with α-GFP. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-GFP. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 9 and 10: Samples described in Figure 3B. Dataset 14: Raw data of western blot displayed in Figure 3C probed with α-GFP. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-GFP. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 5 and 6: Samples described in Figure 3C.