DOI: 10.22002/71crb-2hn94
title: E.cloacae-typeV-CdiA+

description: Type V secretion system
description: <strong>Tilt Series Date:</strong> 2014-03-03</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Drs. Davod Low and Chris Hayes, UC Santa Barbara</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning</p>
<strong>Sample Preparation:</strong> Blot time 3s, Blot off-set -4, 3 ul culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-9/rawdata/</p>atlas10009.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-9/rawdata/atlas10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10009_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-9/3dimage_39530/atlas10009_part121_2.rec"
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        psa2014-03-03-9_slicer19351.png,  filaments tubes vesicles connected, 1.4 MB  
        
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        psa2014-03-03-9_slicer19352.png,  magnetosome?, 1.4 MB  
        
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        psa2014-03-03-9_slicer19353.png,  vesicles with filaments, 1.4 MB  
        
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        psa2014-03-03-9_slicer19354.png,  vesicles with filaments, 1.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2014-03-03-9_slicer19354.png"
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        psa2014-03-03-9_slicer19355.png,  vesicles with filaments, 1.4 MB  
        
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