DOI: 10.5061/dryad.z08kprrq7
title: ITS dataset for Sumacoa (Gesneriaceae), an arborescent new genus from the Eastern Andean slopes of Ecuador

description: A new neotropical genus, Sumacoa, with a single species, S. barbata J. L.
 Clark & D. A. Neill, is described from the Cordillera del Cóndor,
 Cordillera Galeras, and eastern slopes of the Andes in Ecuador and is
 placed in the family Gesneriaceae and tribe Beslerieae. The placement of
 Sumacoa in the tribe Beslerieae is strongly supported by molecular
 sequence data generated from analyses of nuclear ribosomal DNA internal
 transcribed spacer region (ITS). Sequence data were evaluated from 34 taxa
 representing all genera from the tribes Beslerieae and Napeantheae.
 Sumacoa is characterized by the following unique combination of relatively
 uncommon characters in the Gesneriaceae: arborescent habit, a tardily
 dehiscent globose bivalved capsule, and elongate multicellular trichomes
 clustered apically in the throat. Sumacoa is endemic to the provinces of
 Napo and Morona-Santiago and in lower montane cloud forest from the
 Cordillera del Cóndor and Cordillera Galeras at 1200–1800 m elevation, as
 well as one locality on the eastern slopes of the Eastern Cordillera of
 the Andes. A key and a table are presented for differentiating Sumacoa
 from other genera. Based on IUCN guidelines, a preliminary conservation
 status of Vulnerable (VU) is provided for S. barbata.
description: <strong>DNA Extraction, Amplification, and
 Sequencing</strong>—All the tissue samples included in the analysis
 were collected in the field and dried in silica gel. Leaf samples were
 ground using a ThermSavant FastPrep FP120 cell disrupter (Qbiogene,
 Carlsbad, California). DNA was isolated using the Qiagen DNeasyTM DNA
 isolation kit (Qiagen, Valencia, California). 
 Templates of the nrDNA internal transcribed spacer region (ITS)
 were prepared using the primers ITS5HP (5’ –GGA AGG AGA AGT CGT AAC AAG
 G-3’; Suh et al. 1993) and ITS4 (5’ –TCC TCC GCT TAT TGA TAT GC-3’; White
 et al. 1990).  Polymerase chain reaction (PCR)
 amplifications followed the procedures described by Baldwin et al. (1995)
 utilizing Taq DNA polymerase (Promega, Madison, Wisconsin). To reduce
 within-strand base pairing that can result in interference with Taq
 polymerase activity, we found it essential to use 5% DMSO and 5% BSA in
 PCR reactions for ITS. The PCR products were electrophoresed using a 1.0%
 agarose gel in 13 TBE (pH 8.3) buffer, stained with ethidium bromide to
 confirm a single product, and purified using PEG 8000 (polyethylene
 glycol) in 2.5 M NaCl under the conditions described in Johnson and Soltis
 (1995). Direct cycle sequencing of purified template DNAs followed the
 manufacturer’s specifications using the ABI PrismVR BigDyeTM Terminator
 Cycle Sequencing Ready Reaction Kit (PE Biosystems, Foster City,
 California).  DNA chromatograms were edited and contigs
 were assembled using Sequencher version 5.4.6 (Gene Codes Corporation, Ann
 Arbor, Michigan). The sequences include only ITS1, 5.8S, and ITS2 regions.
 Identification of the ends of ITS1, ITS2, and 5.8S were determined by
 comparisons with sequences of <em>Daucus carota</em> and
 <em>Vicia faba</em> from Yokota et al. (1989). All sequence
 data are available in GenBank.
description: # ITS dataset for *Sumacoa* (Gesneriaceae), an arborescent new genus from
 the eastern Andean slopes of Ecuador
 [https://doi.org/10.5061/dryad.z08kprrq7](https://doi.org/10.5061/dryad.z08kprrq7) ## Description of the data and file structure ***DNA Sequencing and Alignment***—The ITS region produced overlapping fragments that collectively covered the entire spacer and 5.8S rDNA regions along both strands. The aligned ITS data matrix was 688 base pairs (bp) long with 373 (54%) variable sites, of which 268 (39%) were parsimony informative. The two outgroups of the analysis contributed 16 of the 268 parsimony informative characters. There were no ambiguously aligned sites excluded from the analysis.