cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7898357.v1
title: Etiology, occurrence and epidemiology of a begomovirus disease in passionflower in the southwest of Bahia

description: ABSTRACT: Severe mosaic symptoms, accompanied by yellow spots, abnormally small leaves, fruit malformation and cracking, reduced plant growth, and high levels of whitefly (Bemisia tabaci MEAM1) infestation were observed in passionflower (Passiflora edulis) orchards in southwestern Bahia, Brazil. The aim of this work was to identify the species of begomovirus infecting the passionflowers, its prevalence in southwestern Bahia, and the spatial and temporal dynamics of the disease. Leaf samples from symptomatic plants collected at 57 orchards located in ten counties were evaluated by PCR for begomovirus infection. Complete nucleotide sequences of DNA-A for two isolates revealed 97 % identity with Passionfruit severe leaf distortion virus (PSLDV). The occurrence of PSLDV in 57 orchards was evaluated based on the presence of characteristic disease symptoms. Approximately 235,000 visually assessed plants exhibited symptoms characteristic of begomovirus infection. Epidemiological studies, conducted in two orchards in Dom Basílio County, showed that disease progress was relatively slow until 121 days after transplanting (DAT), but more rapid in the following 35 days, reaching 100 % infected plants by 156 DAT. The exponential model was fitted to the temporal dynamic of the disease for both areas. An aggregated pattern of diseased plants was predominant for almost all evaluations. It is possible that the primary and secondary spread of the pathogen occurred concurrently during the epidemic progression in both areas, especially late in the season. Containment measures to prevent the virus and the vector from spreading to other passionfruit producing areas in Brazil should be implemented.
DOI: 10.6084/m9.figshare.7898360.v1
title: Effects of perinatal exposure to nonylphenol on delivery outcomes of pregnant rats and inflammatory hepatic injury in newborn rats

description: The current study aimed to investigate the effects of perinatal exposure to nonylphenol (NP) on delivery outcome of pregnant rats and subsequent inflammatory hepatic injury in newborn rats. The pregnant rats were divided into 2 groups: control group (corn oil) and NP exposure group. Thirty-four pregnant rats were administered NP or corn oil by gavage from the sixth day of pregnancy to 21 days postpartum, with blood samples collected at 12 and 21 days of pregnancy and 60 days after delivery. The NP concentration was measured by HPLC, with chemiluminescence used for detection of estrogen and progesterone levels. Maternal delivery parameters were also observed. Liver and blood of the newborn rats were collected and subjected to automatic biochemical detection of liver function and blood lipid analyzer (immunoturbidimetry), and ultrastructural observation of the hepatic microstructure, with the TNF-α and IL-1β hepatic tissue levels evaluated by immunohistochemistry. Compared with the control group, the pregnant and postpartum serum NP and estradiol levels of the mother rats in the NP group were significantly increased, together with lowered progesterone level, increased number of threatened abortion and dystocia, and fewer newborn rats and lower litter weight. Serum and hepatic NP levels of the newborn rats measured 60 days after birth were significantly higher than those of the control group, as well as lower testosterone levels and increased estradiol levels. When observed under electron microscope, the hepatocyte nuclei of the control group were large and round, with evenly distributed chromatin. The chromatin of hepatocytes in the NP group presented deep staining of the nuclei, significant lipid decrease in the cytoplasm, and the majority of cells bonded with lysate. The results of immunohistochemistry showed that there was almost no TNF-α or IL-1β expression in the hepatocytes of the control group, while the number of TNF-α-, PCNA-, and IL-1β-positive cells in the NP group was increased, with higher integral optical density than the control group. Compared to the control group, the serum levels of alanine aminotransferase, aspartate aminotransferase, triglyceride and low-density lipoprotein in the newborn rats of the NP group were significantly increased. There was no significant difference in the serum level of high-density lipoprotein or cholesterol between the groups. Perinatal exposure to NP can interfere with the in vivo estrogen and progesterone levels of pregnant rats, resulting in threatened abortion, dystocia and other adverse delivery outcomes. High liver and serum NP levels of the newborn rats led to alteration of liver tissue structure and function. The NP-induced hepatotoxicity is probably mediated by inflammatory cytokines TNF-α and IL-1α.
DOI: 10.6084/m9.figshare.7898339
title: Peer instruction to address alternative conceptions in Einstein's special relativity

description: Abstract In education, decades have been spent investigating what kind of beliefs and attitudes students may have in relation to learning. The relevance of these lies on the implications that it brings concerning students' alternative conceptions. This research focuses on the inclusion of Peer Instruction (PI) as a methodology to address the alternative conceptions in Einstein's special relativity. PI was selected since there is evidence that it has an impact on the academic performance of the students, however, concerning the topic of relativity, it has not been previously proposed. Alternative Conception Einstein's Special Relativity Test (ACESRT) is proposed to determine students' alternative conceptions. The sample for this study consisted of 25 high school students from Ecuador. In none of this level, students study Einstein's special relativity, nevertheless, in the current year a special course with senior students (picked randomly) was open in which the topic was considered in their academic curriculum. In the first lecture, the students filled a survey to get a notion of their beliefs and attitudes. Then a pre-test (ACESRT) take place, with a view getting information of alternative conceptions that students may have. Later, lectures following PI were given. At the end, to measure PI impact on students' attitudes, beliefs and alternative conceptions the students filled again a survey, followed by post-test (ACESRT). The results were statistically analyzed, and they reveal that PI has a strong impact on students' beliefs and attitudes. Furthermore, the methodology has the potential to address the alternative conception in concepts related to the topic Einstein's special relativity.
DOI: 10.6084/m9.figshare.7898342
title: Obesity inversely correlates with prostate-specific antigen levels in a population with normal screening results of prostate cancer in northwestern China

description: Serum prostate-specific antigen (PSA) is a diagnostic biomarker of prostate cancer and is possibly associated with obesity. This study aimed to explore the relationships between obesity indicators [body mass index (BMI) and waist circumference (WC)] with PSA in Chinese men. A cross-sectional study of men aged 30-85 years undergoing prostate cancer screening was conducted from August 2008 to July 2013 in Xi'an, China. Data were obtained from clinical reports, condition was recorded based on self-report including demographics, weight, height, and WC (&gt;90 cm=obese). Fasting blood glucose (FBG) and prostate volume (PV) were assessed clinically. Patients were grouped by BMI (normal=22.9, overweight=23-27.4, obese≥27.5 kg/m2). PSA parameters of density (PSAD), PSA serum level, and PSA increasing rate per year (PSAR) were calculated per BMI and age groups (30-40, 41-59, 60-85 years). Obesity indicators (BMI and WC) and PSA parameter relationships were modeled by age-stratified linear regression. Of 35,632 Chinese men surveyed, 13,084 were analyzed, including 13.44% obese, 57.44% overweight, and 29.12% normal weight, according to BMI; 25.84% were centrally (abdominally) obese according to WC. BMI and WC were negatively associated with all PSA parameters, except PSAD and PSAR [P&lt;0.05, BMI: β=-0.081 (95%CI=-0.055 to -0.036), WC: β=-0.101 (-0.021 to -0.015)], and independent of FBG and PV (P&lt;0.05) in an age-adjusted model. In conclusion, obesity was associated with lower PSA in Chinese men. Therefore, an individual's BMI and WC should be considered when PSA is used to screen for prostate cancer.
DOI: 10.6084/m9.figshare.7898354.v1
title: Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro

description: Abstract: Medicinal plants have long been used as an alternative to traditional drugs for the treatment of inflammatory conditions due to the classical side effects and restricted access of various commercially available drugs, such as steroids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs). Sambucus australis is a Brazilian herb that is commonly used to treat inflammatory diseases; however, few studies have examined the use of this species in the treatment of inflammatory conditions. The present study aims to evaluate the potential anti-inflammatory activity of S. australis in vitro. We established spleen cell cultures stimulated with pokeweed mitogen (PWM) to evaluate the production of proinflammatory cytokines, such as IL-4, IL-5, IFN-y, and IL-10 (by ELISA), and the expression of the transcription factor NF-kB (by RT-PCR). In addition, we evaluated the levels of nitric oxide in macrophage cultures and the membrane-stabilizing activity of S. australis methanolic extract (EMSA). Treatment with EMSA at concentrations of 100, 50, 25 and 12.5 µg/ml significantly decreased IL-4 (p&lt;0.001) and IL-5 (p&lt;0.001) levels. Treatment with 100 µg/ml EMSA reduced IFN-у (p&lt;0.001) levels. Moreover, at 100 mg/ml, EMSA also increased IL-10 production and reduced NF-kB expression (p&lt;0.01). In macrophage cultures stimulated with LPS, EMSA decreased nitric oxide levels (p&lt;0.001) at all concentrations tested (100, 50, 25 and 12.5 µg/ml). Additionally, EMSA had a protective effect in the erythrocyte membrane stabilization assay. Taken together, these results suggest that S. australis has anti-inflammatory potential in vitro, characterized by the reduction of both inflammatory cytokines and the expression of NF-kB along with the up-regulation of IL-10.
DOI: 10.6084/m9.figshare.7898330
title: Treatment of hepatitis C virus genotype 3 infection with direct-acting antiviral agents

description: Hepatitis C virus (HCV) genotype 3 is responsible for 30.1% of chronic hepatitis C infection cases worldwide. In the era of direct-acting antivirals, these patients have become one of the most challenging to treat, due to fewer effective drug options, higher risk of developing cirrhosis and hepatocellular carcinoma and lower sustained virological response (SVR) rates. Currently there are 4 recommended drugs for the treatment of HCV genotype 3: pegylated interferon (PegIFN), sofosbuvir (SOF), daclatasvir (DCV) and ribavirin (RBV). Treatment with PegIFN, SOF and RBV for 12 weeks has an overall SVR rate of 83–100%, without significant differences among cirrhotic and non-cirrhotic patients. However, this therapeutic regimen has several contraindications and can cause significant adverse events, which can reduce adherence and impair SVR rates. SOF plus RBV for 24 weeks is another treatment option, with SVR rates of 82–96% among patients without cirrhosis and 62–92% among those with cirrhosis. Finally, SOF plus DCV provides 94–97% SVR rates in non-cirrhotic patients, but 59–69% in those with cirrhosis. The addition of RBV to the regimen of SOF plus DCV increases the SVR rates in cirrhotic patients above 80%, and extending treatment to 24 weeks raises SVR to 90%. The ideal duration of therapy is still under investigation. For cirrhotic patients, the optimal duration, or even the best regimen, is still uncertain. Further studies are necessary to clarify the best regimen to treat HCV genotype 3 infection.
DOI: 10.6084/m9.figshare.7898333
title: Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia

description: We evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia received an oral glucose solution (75 g) Blood was collected at 0 (baseline), 30, 60, 120 and 180 min after glucose ingestion and the plasma concentrations of interferon-α (IFN-α), interferon-γ (IFN-γ), interleukin-1 receptor antagonist (IL-1RA), interleukin 2 (IL-2), interleukin-2 receptor (IL-2R), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin-12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), IFN-γ inducible protein 10 (IP-10), monocyte chemotactic protein 1 (MCP1), monokine induced by IFN-γ (MIG), macrophage inflammatory protein-1α (MIP-1α), interleukin-1β (IL-1β), colony stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), basic fibroblast growth factor (FGF-basic), eotaxin, tumor necrosis factor α (TNFα), epidermal growth factor (EGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), macrophage inflammatory protein-1α (MIP-1α), and 1β (MIP-1β) were evaluated. Overall, glycemic levels increased, reached its maximum at 30 min (phase 1), returned to baseline levels at 120 min (phase 2), followed by a mild hypoglycemia at 180 min (phase 3). During phase 1, cytokine blood levels were maintained. However, we observed a synchronous fall (P&lt;0.05) in the concentrations of pro-inflammatory (IL-15, IL-17, MCP-1) and anti-inflammatory cytokines (FGF-basic, IL-13, IL-1RA) during phase 2. Furthermore, a simultaneous rise (P&lt;0.05) of pro-inflammatory (IL-2, IL-5, IL-17) and anti-inflammatory cytokines (IL-4, IL-1RA, IL-2R, IL-13, FGF-basic) occurred during phase 3. Thus, mild acute hypoglycemia but not a physiological increase of glycemia was associated with increased blood levels of anti-inflammatory and pro-inflammatory cytokines.
DOI: 10.6084/m9.figshare.7898336
title: Clinical and prognostic significance of serum transforming growth factor-beta1 levels in patients with pancreatic ductal adenocarcinoma

description: Pancreatic ductal adenocarcinoma (PDAC) has a poor 5-year survival rate of 5%. Biomarkers for the early detection of pancreatic cancer are urgently needed. Transforming growth factor-beta1 (TGF-β1) is elevated in the tissues and plasma of patients with PDAC. However, no studies systemically report prognostic significance of plasma TGF-β1 levels in PDAC. In the present study, we assessed the prognostic significance of serum TGF-β levels in patients with PDAC. TGF-β levels were determined in serum from 146 PDAC patients, and 58 patients with benign pancreatic conditions. Regression models were used to correlate TGF-β levels to gender, age, stage, class, and metastasis. Survival analyses were performed using multivariate Cox models. Serum levels of TGF-β1 distinguished PDAC from benign pancreatic conditions (P&lt;0.001) and healthy control subjects (P&lt;0.001). Serum levels of TGF-β also distinguished tumor stage (P=0.002) and lymph node metastasis (P=0.001). High serum levels of TGF-β1 were significantly correlated with reduced patient survival. Multivariate analysis revealed that TGF-β1, lymph node metastasis and tumor stage were independent factors for PDAC survival. Our results indicate that serum TGF-β1 may be used as a potential prognostic marker for PDAC.
DOI: 10.6084/m9.figshare.7898339.v1
title: Peer instruction to address alternative conceptions in Einstein's special relativity

description: Abstract In education, decades have been spent investigating what kind of beliefs and attitudes students may have in relation to learning. The relevance of these lies on the implications that it brings concerning students' alternative conceptions. This research focuses on the inclusion of Peer Instruction (PI) as a methodology to address the alternative conceptions in Einstein's special relativity. PI was selected since there is evidence that it has an impact on the academic performance of the students, however, concerning the topic of relativity, it has not been previously proposed. Alternative Conception Einstein's Special Relativity Test (ACESRT) is proposed to determine students' alternative conceptions. The sample for this study consisted of 25 high school students from Ecuador. In none of this level, students study Einstein's special relativity, nevertheless, in the current year a special course with senior students (picked randomly) was open in which the topic was considered in their academic curriculum. In the first lecture, the students filled a survey to get a notion of their beliefs and attitudes. Then a pre-test (ACESRT) take place, with a view getting information of alternative conceptions that students may have. Later, lectures following PI were given. At the end, to measure PI impact on students' attitudes, beliefs and alternative conceptions the students filled again a survey, followed by post-test (ACESRT). The results were statistically analyzed, and they reveal that PI has a strong impact on students' beliefs and attitudes. Furthermore, the methodology has the potential to address the alternative conception in concepts related to the topic Einstein's special relativity.
DOI: 10.6084/m9.figshare.7898342.v1
title: Obesity inversely correlates with prostate-specific antigen levels in a population with normal screening results of prostate cancer in northwestern China

description: Serum prostate-specific antigen (PSA) is a diagnostic biomarker of prostate cancer and is possibly associated with obesity. This study aimed to explore the relationships between obesity indicators [body mass index (BMI) and waist circumference (WC)] with PSA in Chinese men. A cross-sectional study of men aged 30-85 years undergoing prostate cancer screening was conducted from August 2008 to July 2013 in Xi'an, China. Data were obtained from clinical reports, condition was recorded based on self-report including demographics, weight, height, and WC (&gt;90 cm=obese). Fasting blood glucose (FBG) and prostate volume (PV) were assessed clinically. Patients were grouped by BMI (normal=22.9, overweight=23-27.4, obese≥27.5 kg/m2). PSA parameters of density (PSAD), PSA serum level, and PSA increasing rate per year (PSAR) were calculated per BMI and age groups (30-40, 41-59, 60-85 years). Obesity indicators (BMI and WC) and PSA parameter relationships were modeled by age-stratified linear regression. Of 35,632 Chinese men surveyed, 13,084 were analyzed, including 13.44% obese, 57.44% overweight, and 29.12% normal weight, according to BMI; 25.84% were centrally (abdominally) obese according to WC. BMI and WC were negatively associated with all PSA parameters, except PSAD and PSAR [P&lt;0.05, BMI: β=-0.081 (95%CI=-0.055 to -0.036), WC: β=-0.101 (-0.021 to -0.015)], and independent of FBG and PV (P&lt;0.05) in an age-adjusted model. In conclusion, obesity was associated with lower PSA in Chinese men. Therefore, an individual's BMI and WC should be considered when PSA is used to screen for prostate cancer.
DOI: 10.6084/m9.figshare.7898324
title: Surgical treatment of Denis type B thoracolumbar burst fracture with neurological deficiency by paraspinal approach

description: We aimed to describe the surgical technique and clinical outcomes of paraspinal-approach reduction and fixation (PARF) in a group of patients with Denis type B thoracolumbar burst fracture (TLBF) with neurological deficiencies. A total of 62 patients with Denis B TLBF with neurological deficiencies were included in this study between January 2009 and December 2011. Clinical evaluations including the Frankel scale, pain visual analog scale (VAS) and radiological assessment (CT scans for fragment reduction and X-ray for the Cobb angle, adjacent superior and inferior intervertebral disc height, and vertebral canal diameter) were performed preoperatively and at 3 days, 6 months, and 1 and 2 years postoperatively. All patients underwent successful PARF, and were followed-up for at least 2 years. Average surgical time, blood loss and incision length were recorded. The sagittal vertebral canal diameter was significantly enlarged. The canal stenosis index was also improved. Kyphosis was corrected and remained at 8.6±1.4o (P&gt;0.05) 1 year postoperatively. Adjacent disc heights remained constant. Average Frankel grades were significantly improved at the end of follow-up. All 62 patients were neurologically assessed. Pain scores decreased at 6 months postoperatively, compared to before surgery (P&lt;0.05). PARF provided excellent reduction for traumatic segmental kyphosis, and resulted in significant spinal canal clearance, which restored and maintained the vertebral body height of patients with Denis B TLBF with neurological deficits.
DOI: 10.6084/m9.figshare.7898327
title: An overview of inventories of gall-inducing insects in Brazil: looking for patterns and identifying knowledge gaps

description: We compiled published Brazilian gall-inducing insect inventories aiming to understand trends and biases in this field research and to investigate the factors that potentially explain the diversity of gall-inducing insects among different sampling sites. A total of 51 studies with gall-inducing insect inventories were compiled for Brazil, which sampled 151 sites in 88 municipalities, 13 states and five regions. The number of papers published on gall-inducing insects per year has increased over the last 30 years, being Cecidomyiidae (Diptera) the main galling taxon, Fabaceae the main host-plant family and Protium heptaphyllum (Burseraceae) the most important super-host species in these inventories. We found a great bias in the geographical distribution of Brazilian inventories, with the majority of studies in the Southeast region, and Atlantic Forest and Cerrado biomes. The total richness of gall-inducing insects differed significantly among regions and biomes, with higher gall richnesses being recorded in the North region and Amazon biome. However, Brazilian regions and biomes did not vary in richness of gall-inducing insect morphotypes per plant species. According our results, sampling by cecidologists in less studied regions of Brazil is needed, particularly in the North and South regions and subsampled biomes such as the Amazon, Pampas and Pantanal.