cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.5811851
title: _A_SxPdCasEVNonGuidedPathway-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T22_SxPv2\_S_P1_TransgenicStableSxPv2\_A_SxPdCasEVNonGuidedPathway-GCMS\output\raw


Title:    SxP dCasEV Non Guided Pathway (EV NGP)
Description:    Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promoters, the dCasEV system and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the specific guide in trans, so the system is complete and the production of the pheromones is triggered.
DOI: 10.5281/zenodo.5811866
title: _A_SxPNonGuidedPathway-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T22_SxPv2\_S_P1_TransgenicStableSxPv2\_A_SxPNonGuidedPathway-GCMS\output\raw


Title:    SxP Non Guided Pathway (NGP)
Description:    Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promoters and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the dCasEV and the specific guide in trans, so the system is complete and the production of the pheromones is triggered.
DOI: 10.5281/zenodo.5811867
title: _A_SxPNonGuidedPathway-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T22_SxPv2\_S_P1_TransgenicStableSxPv2\_A_SxPNonGuidedPathway-GCMS\output\raw


Title:    SxP Non Guided Pathway (NGP)
Description:    Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promoters and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the dCasEV and the specific guide in trans, so the system is complete and the production of the pheromones is triggered.
DOI: 10.15468/dl.pe6bb8
title: Occurrence Download

description: A dataset listing the 3484 species recorded in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Specimen, Occurrence evidence)",
 "Country is United States of America",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is one of (Colletidae, Andrenidae, Apidae, Halictidae, Megachilidae, Melittidae)",
 "Year 1970-2025"
 ]
}

 The dataset's 3484 records were derived from 167 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000056-250324175209820/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.6554065
title: _A_TransientCompLPPSCPPS-GCMS.output.raw

description: pISA-tree file path:   _p_SUSPHIRE\_I_T32_CandidateGeneExpressionTesting\_S_TransientLPPSCPPS\_A_TransientCompLPPSCPPS-GCMS\output\raw


Title:    Transient expression of LPPS and CPPS genes in Nicotiana benthamiana plants
Description:    The purpose of this assay is to check the transient expression of the LPPS and CPPS genes in Nicotiana benthamiana plants. Two different Agrobacterium strains were assayed and compared the levels of production between them. Volatile monoterpenoid levels were measured by GCMS of samples.
DOI: 10.5281/zenodo.6554066
title: _A_TransientCompLPPSCPPS-GCMS.output.raw

description: pISA-tree file path:   _p_SUSPHIRE\_I_T32_CandidateGeneExpressionTesting\_S_TransientLPPSCPPS\_A_TransientCompLPPSCPPS-GCMS\output\raw


Title:    Transient expression of LPPS and CPPS genes in Nicotiana benthamiana plants
Description:    The purpose of this assay is to check the transient expression of the LPPS and CPPS genes in Nicotiana benthamiana plants. Two different Agrobacterium strains were assayed and compared the levels of production between them. Volatile monoterpenoid levels were measured by GCMS of samples.
DOI: 10.15468/dl.nuurvv
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Dussia cuscatlanica (Standl.) Standl. & Steyerm.)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 21 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000072-250324175209820/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.6559100
title: _A_LA_liq-GC-MS.output.raw

description: pISA-tree file path:   _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lav_ac\_A_LA_liq-GC-MS\output\raw\


Title:    Detection of lavandulyl acetate in extracts of SxF liquid cultures
Description:    Detection of lavandulyl acetate in solvent extracts of SxF liquid cultures by GC-MS-MS
DOI: 10.5281/zenodo.6559101
title: _A_LA_liq-GC-MS.output.raw

description: pISA-tree file path:   _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lav_ac\_A_LA_liq-GC-MS\output\raw\


Title:    Detection of lavandulyl acetate in extracts of SxF liquid cultures
Description:    Detection of lavandulyl acetate in solvent extracts of SxF liquid cultures by GC-MS-MS
DOI: 10.5281/zenodo.6559449
title: _A_L_liq-GC-MS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lavandulol\_A_L_liq-GC-MS\output\raw


Title:    Detection of lavandulol in extracts of SxF liquid cultures
Description:    Detection of lavandulol in solvent extracts of SxF liquid cultures by GC-MS-MS
DOI: 10.5281/zenodo.6559450
title: _A_L_liq-GC-MS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lavandulol\_A_L_liq-GC-MS\output\raw


Title:    Detection of lavandulol in extracts of SxF liquid cultures
Description:    Detection of lavandulol in solvent extracts of SxF liquid cultures by GC-MS-MS
DOI: 10.5281/zenodo.6559480
title: _A_L_myc-GC-MS.output.raw

description: pISA-tree file path:  _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lavandulol\_A_L_myc-GC-MS


Title:    Dectection of lavandulol in SxF mycelium extracts
Description:    Detection of lavandulol in solvent extracts of SxF mycelia by GC-MS-MS
DOI: 10.5281/zenodo.6559481
title: _A_L_myc-GC-MS.output.raw

description: pISA-tree file path:  _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lavandulol\_A_L_myc-GC-MS


Title:    Dectection of lavandulol in SxF mycelium extracts
Description:    Detection of lavandulol in solvent extracts of SxF mycelia by GC-MS-MS
DOI: 10.5281/zenodo.6575633
title: _A_T0NbenthLPPSCPPS1stround-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T0NbenthLPPSCPPS1stround-GCMS\output\raw


Title:    Analysis of the first round of T0 generation LPPS and CPPS Nicotiana benthamiana plants
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the generated T0 transgenic plants of Nicotiana benthamiana after transformation with the LPPS or CPPS gene. This analysis of monoterpenoids production is performed by HSPME. This is the analysis for the first round of transgenic plants transfered to soil.
DOI: 10.5281/zenodo.6575634
title: _A_T0NbenthLPPSCPPS1stround-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T0NbenthLPPSCPPS1stround-GCMS\output\raw


Title:    Analysis of the first round of T0 generation LPPS and CPPS Nicotiana benthamiana plants
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the generated T0 transgenic plants of Nicotiana benthamiana after transformation with the LPPS or CPPS gene. This analysis of monoterpenoids production is performed by HSPME. This is the analysis for the first round of transgenic plants transfered to soil.
DOI: 10.5281/zenodo.6575691
title: _A_T0NbenthLPPSCPPS2ndround-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T0NbenthLPPSCPPS2ndround-GCMS\output\raw


Title:    Analysis of the second round of T0 generation LPPS and CPPS Nicotiana benthamiana plants
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the generated T0 transgenic plants of Nicotiana benthamiana after transformation with the LPPS or CPPS gene. This analysis of monoterpenoids production is performed by HSPME. This is the analysis for the second round of transgenic plants transfered to soil.
DOI: 10.5281/zenodo.6575692
title: _A_T0NbenthLPPSCPPS2ndround-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T0NbenthLPPSCPPS2ndround-GCMS\output\raw


Title:    Analysis of the second round of T0 generation LPPS and CPPS Nicotiana benthamiana plants
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the generated T0 transgenic plants of Nicotiana benthamiana after transformation with the LPPS or CPPS gene. This analysis of monoterpenoids production is performed by HSPME. This is the analysis for the second round of transgenic plants transfered to soil.
DOI: 10.5281/zenodo.5514205
title: Philosophy [IO Bijapur 146, 167] Glosses on الهداية, Commentary on الشمسية

description: Philosophy.

This manuscript is now IO Bijapur 146, 167 in the India Office collections.

[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 584 here with notations and hyperlinks].


584.

B 146, 167. Size 7 in. by 4 in.; foll. 151. Seventeen lines in a page.

I. Foll. 1-71. SAIYID SHARÎF’S Glosses on Ḳuṭb al-dîn’s Commentary on the Shamsîyah (see no. 507).

The latter portion wanting. Eight leaves missing after fol. 31.

II. Foll. 72-151. MÎRAK’s Commentary on the Hidâyah (see no. 493).

The first leaf missing. Begins:

و تصير عالَماً

Both pieces are boldly written, by Yûsuf b. Muḥammad b. Yûsuf Zauzanî. The second is dated 4th Ṣafar, 825 [=28 January 1422]. Foll. 110 sqq. Are worm-eaten, but the text is not injured.

[Bijapur Collection]
DOI: 10.5281/zenodo.5514204
title: Philosophy [IO Bijapur 146, 167] Glosses on الهداية, Commentary on الشمسية

description: Philosophy.

This manuscript is now IO Bijapur 146, 167 in the India Office collections.

[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 584 here with notations and hyperlinks].


584.

B 146, 167. Size 7 in. by 4 in.; foll. 151. Seventeen lines in a page.

I. Foll. 1-71. SAIYID SHARÎF’S Glosses on Ḳuṭb al-dîn’s Commentary on the Shamsîyah (see no. 507).

The latter portion wanting. Eight leaves missing after fol. 31.

II. Foll. 72-151. MÎRAK’s Commentary on the Hidâyah (see no. 493).

The first leaf missing. Begins:

و تصير عالَماً

Both pieces are boldly written, by Yûsuf b. Muḥammad b. Yûsuf Zauzanî. The second is dated 4th Ṣafar, 825 [=28 January 1422]. Foll. 110 sqq. Are worm-eaten, but the text is not injured.

[Bijapur Collection]
DOI: 10.5281/zenodo.6575703
title: _A_T1NbenthLPPSCPPSAcetyltransferase-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T1NbenthLPPSCPPSAcetyltransferase-GCMS


Title:    Analysis of acetyltated monoterpenoid products in T1 generation LPPS and CPPS Nicotiana benthamiana plants after transient expression of an acetyltransferase gene.
Description:    The purpose of this assay is to analyze the acetylated products in the T1 transgenic generation LPPS and CPPS plants after the transient expression mediated by Agrobacterium of the transgene LiAAT4, from lavandula.
DOI: 10.5281/zenodo.6575704
title: _A_T1NbenthLPPSCPPSAcetyltransferase-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T1NbenthLPPSCPPSAcetyltransferase-GCMS


Title:    Analysis of acetyltated monoterpenoid products in T1 generation LPPS and CPPS Nicotiana benthamiana plants after transient expression of an acetyltransferase gene.
Description:    The purpose of this assay is to analyze the acetylated products in the T1 transgenic generation LPPS and CPPS plants after the transient expression mediated by Agrobacterium of the transgene LiAAT4, from lavandula.
DOI: 10.5281/zenodo.6575734
title: _A_T1NbenthLPPSSCPPS-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T1NbenthLPPSSCPPS-GCMS


Title:    Analysis of the monoterpenoid production of T1 generation LPPS and CPPS Nicotiana benthamiana plants.
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the T1 transgenic plants of Nicotiana benthamiana after transformation with the LPPS or CPPS gene. This analysis of monoterpenoids production is performed by HSPME.
DOI: 10.5281/zenodo.6575735
title: _A_T1NbenthLPPSSCPPS-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NbenthamianaLPPSCPPS\_A_T1NbenthLPPSSCPPS-GCMS


Title:    Analysis of the monoterpenoid production of T1 generation LPPS and CPPS Nicotiana benthamiana plants.
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the T1 transgenic plants of Nicotiana benthamiana after transformation with the LPPS or CPPS gene. This analysis of monoterpenoids production is performed by HSPME.
DOI: 10.5281/zenodo.6575760
title: _A_T1NtabacumLPPSCPPS-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NtabacumLPPSCPPS\_A_T1NtabacumLPPSCPPS-GCMS\output\raw


Title:    Analysis of the T1 generation of LPPS and CPPS Nicotiana tabacum plants
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the generated T1 transgenic plants of Nicotiana tabacum after transformation with the LPPS or CPPS gene. The analysis of monoterpenoid production was performed by HSPME, and a growth phenotyping of plants was also performed.
DOI: 10.5281/zenodo.6575761
title: _A_T1NtabacumLPPSCPPS-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsPlants\_S_P1_NtabacumLPPSCPPS\_A_T1NtabacumLPPSCPPS-GCMS\output\raw


Title:    Analysis of the T1 generation of LPPS and CPPS Nicotiana tabacum plants
Description:    The purpose of this assay is to analyze the lavandulol, chrysanthemol and other monoterpenoids derivatives production in the generated T1 transgenic plants of Nicotiana tabacum after transformation with the LPPS or CPPS gene. The analysis of monoterpenoid production was performed by HSPME, and a growth phenotyping of plants was also performed.
DOI: 10.5281/zenodo.5514199
title: Philosophy [IO Bijapur 170]

description: Philosophy.

This manuscript is now IO Bijapur 170 in the India Office collections.

[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 583 here with further notations and hyperlinks].


583.

B 170. Size 81/4 in. by 53/4 in.; foll. 72. Mostly nineteen lines in a page.

I.Foll. 1-24. The beginning of MAIBUDHÎ’S شرح الهداية (see no. 487).

Plainly written, with marginal notes. Stained, and injured on the margin.

II.Foll. 25-75. A fragment of MÎRAK’S شرح حكمة العين (see no. 498).

It begins soon after the commencement of the work, with the words الشدة و الضعف.

Well written. The margin covered with glosses, and in better preservation than that of I.
DOI: 10.5281/zenodo.5514200
title: Philosophy [IO Bijapur 170]

description: Philosophy.

This manuscript is now IO Bijapur 170 in the India Office collections.

[metadata: Otto Loth, A Catalogue of the Arabic Manuscripts in the Library of the India Office, (volume 1), no. 583 here with further notations and hyperlinks].


583.

B 170. Size 81/4 in. by 53/4 in.; foll. 72. Mostly nineteen lines in a page.

I.Foll. 1-24. The beginning of MAIBUDHÎ’S شرح الهداية (see no. 487).

Plainly written, with marginal notes. Stained, and injured on the margin.

II.Foll. 25-75. A fragment of MÎRAK’S شرح حكمة العين (see no. 498).

It begins soon after the commencement of the work, with the words الشدة و الضعف.

Well written. The margin covered with glosses, and in better preservation than that of I.
DOI: 10.5281/zenodo.5810414
title: _A_06_SxPv1-0_Illumina-Centrifuge.output

description: pISA-tree file path:    _p_SUSPHIRE\_I_T21_SXPsysbio\_S_P4_SxP10-newG-DE\_A_06_SxPv1-0_Illumina-Centrifuge\output\Centrifuge_outputs


Title:    centrifuge analysis of reads from SxPv1.0 samples
DOI: 10.5281/zenodo.5810415
title: _A_06_SxPv1-0_Illumina-Centrifuge.output

description: pISA-tree file path:    _p_SUSPHIRE\_I_T21_SXPsysbio\_S_P4_SxP10-newG-DE\_A_06_SxPv1-0_Illumina-Centrifuge\output\Centrifuge_outputs


Title:    centrifuge analysis of reads from SxPv1.0 samples
DOI: 10.5281/zenodo.15078203
title: Supplement for "Handling Imperfection: A Taxonomy for Machine Learning on Data with Data Quality Defects"
DOI: 10.5281/zenodo.15078204
title: Supplement for "Handling Imperfection: A Taxonomy for Machine Learning on Data with Data Quality Defects"
DOI: 10.5281/zenodo.5810286
title: _A_AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS\output\raw


Title:    Agroinfiltration of nonguided pathway for moth pheromones production with constitutive dCAs9EV system in Nicotiana benthamiana leaves
Description:    Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system expressed constitutively and a plasmid containing the guideRNA1 or another guideRNA as a negative control. Leaf samples were collected after 5 days and the pheromone production was assayed via GC-MS
DOI: 10.5281/zenodo.5810287
title: _A_AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS\output\raw


Title:    Agroinfiltration of nonguided pathway for moth pheromones production with constitutive dCAs9EV system in Nicotiana benthamiana leaves
Description:    Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system expressed constitutively and a plasmid containing the guideRNA1 or another guideRNA as a negative control. Leaf samples were collected after 5 days and the pheromone production was assayed via GC-MS
DOI: 10.5281/zenodo.5810278
title: _A_AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS\output\raw


Title:    Agroinfiltration of non-guided pathway for moth pheromones production with copper-inducible dCas9EV system in Nicotiana benthamiana leaves
Description:    Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system under the regulation of a copper-inducible promoter and the CUP2:Gal4 protein. Agroinfiltrated leaves were treated 3 days after infiltration with Cu2SO4 1mM or water for the controls. Leaf samples were collected 2 days after copper treatment and the pheromone production was assayed via GC-MS
DOI: 10.5281/zenodo.5810279
title: _A_AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS\output\raw


Title:    Agroinfiltration of non-guided pathway for moth pheromones production with copper-inducible dCas9EV system in Nicotiana benthamiana leaves
Description:    Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system under the regulation of a copper-inducible promoter and the CUP2:Gal4 protein. Agroinfiltrated leaves were treated 3 days after infiltration with Cu2SO4 1mM or water for the controls. Leaf samples were collected 2 days after copper treatment and the pheromone production was assayed via GC-MS
DOI: 10.5281/zenodo.5810256
title: _A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS\output\raw


Title:    Agroinfiltration of guided pathway for moth pheromones production with constitutive dCas9EV system in Nicotiana benthamiana leaves
Description:    Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. In the constructs it was also included the guideRNA1 and nptII genes for their use in stable transformation. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system expressed constitutively or a plasmid containing just P19 as a negative control. Leaf samples were collected after 5 days and the pheromone production was assayed via GC-MS
DOI: 10.5281/zenodo.5810257
title: _A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS.output.raw

description: pISA-tree file path:    _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS\output\raw


Title:    Agroinfiltration of guided pathway for moth pheromones production with constitutive dCas9EV system in Nicotiana benthamiana leaves
Description:    Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. In the constructs it was also included the guideRNA1 and nptII genes for their use in stable transformation. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system expressed constitutively or a plasmid containing just P19 as a negative control. Leaf samples were collected after 5 days and the pheromone production was assayed via GC-MS