cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/97a3q-sja89
title: Legionella delta DotOB

description: DotF-GFP background
description: <strong>Tilt Series Date:</strong> 2019-02-13</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph Vogel</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase</p>
<strong>Sample Preparation:</strong> 10 nm gold beads, broad vitrobot, R2/2 200 mesh copper quantifoil grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-66/rawdata/</p>20190213_deltaOB_36.mrc,  Tilt Series (Pixel Size 0.27 nm), 227.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-02-13-66/rawdata/20190213_deltaOB_36.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rxds6-zqf08
title: B. diazoefficiens, hopanoids

description: WT strain, stationary phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Broad vitrobot, blot force 6, blot time 3s, drain 1s, wait 0s, 22 degC, 100% humidity.. 4uL of 6X concentrated cell (original O.D. 1.2). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-18/rawdata/</p>WT_ST_70step2_8_2.mrc,  Tilt Series (Pixel Size 2.678 nm), 377.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-18/rawdata/WT_ST_70step2_8_2.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/axrvm-s1648
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-07-10</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, over-express, from Partick, 4331</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-07-10-11/rawdata/</p>targ12.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-07-10-11/rawdata/targ12.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y0n26-awq35
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-178/rawdata/</p>JV9298_PD-1-3_052.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.4 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a9qsg-9qx22
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-55/rawdata/</p>dH_LG_64step2_28.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-55/rawdata/dH_LG_64step2_28.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8zqyqn
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Taeniopygia guttata",
 "Country is Slovenia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006503-250827131500795/datasets/export for details.
DOI: 10.22002/6mdxh-22e75
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-396/rawdata/</p>JV9298_PD-1-3_380.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-396/rawdata/JV9298_PD-1-3_380.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r0qs8-yw349
title: Saccharomyces cerevisiae - 6210

description: Raw data files of Saccharomyces cerevisiae - 6210
description: <strong>Tilt Series Date:</strong> 2017-10-25</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain from Markus Babst @ University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to ~0.6 OD from overnight cultures in YPD.</p>
<strong>Sample Preparation:</strong> added ~3.5 µL of ~0.2 OD cells for each grid and blotted from behind for ~3 sec. FIB milled on Sep25_2017.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-10/rawdata/</p>TS9.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-10-25-10/rawdata/TS9.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wejcm-cz150
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2015-10-19</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> AZ21, ΔsdpABC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Zielinska, A., Billini, M., Moll, A., Kremer, K., Briegel, A., Izquierdo Martinez, A., Jensen, G. J. and Thanbichler, M. (2017). Lytm factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106, 419-438.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-19-35/rawdata/</p>AZ21Set0011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-19-35/rawdata/AZ21Set0011.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0p208-c3m49
title: Pombe Cryosections

description: These cells were high pressure frozen after the majority of the rings were around mid-division.
description: <strong>Tilt Series Date:</strong> 2015-06-03</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Manual/Raptor</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-06-03-26/rawdata/</p>060315_pombe_cryosection2_square8_atlastgt.mrc,  Tilt Series (Pixel Size 0.5 nm), 57.1 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.xpjnvh
title: Occurrence Download

description: A dataset containing 18692 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Haemaphysalis papuana, Ixodes granulatus, Ixodes persulcatus, Haemaphysalis longicornis, Ixodes scapularis, Ornithodoros sonrai)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 18692 records from 70 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006502-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/md71d-xhx85
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-20</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, WT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-52/rawdata/</p>targ71.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-20-52/rawdata/targ71.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.5rkj28
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Chiloglanis carnosus, Chiloglanis congicus, Chiloglanis deckenii, Chiloglanis devosi, Chiloglanis dialloi, Chiloglanis disneyi, Chiloglanis elisabethianus, Chiloglanis emarginatus, Chiloglanis fasciatus, Chiloglanis harbinger, Chiloglanis kabaensis, Chiloglanis kalambo, Chiloglanis kerioensis, Chiloglanis kolente, Chiloglanis lamottei, Chiloglanis loffabrevum, Chiloglanis longibarbis, Chiloglanis lufirae, Chiloglanis lukugae, Chiloglanis macropterus, Chiloglanis marlieri, Chiloglanis mbozi, Chiloglanis microps, Chiloglanis modjensis, Chiloglanis mongoensis, Chiloglanis neumanni, Chiloglanis niger, Chiloglanis niloticus, Chiloglanis normani, Chiloglanis nzerekore, Chiloglanis occidentalis, Chiloglanis paratus, Chiloglanis pezoldi, Chiloglanis pojeri, Chiloglanis polyodon, Chiloglanis polypogon, Chiloglanis pretoriae, Chiloglanis reticulatus, Chiloglanis rukwaensis, Chiloglanis ruziziensis, Chiloglanis sanagaensis, Chiloglanis sardinhai, Chiloglanis somereni, Chiloglanis swierstrai, Chiloglanis trilobatus, Chiloglanis tweddlei, Chiloglanis voltae, Chiloglanis waterloti, Chilotilapia euchilus, Chilotilapia rhoadesii, Chindongo ater, Chindongo bellicosus, Chindongo cyaneus, Chindongo demasoni, Chindongo flavus, Chindongo heteropictus, Maylandia heteropicta, Chindongo longior, Chindongo minutus, Chindongo saulosi, Chromidotilapia cavalliensis, Chromidotilapia elongata, Chromidotilapia guntheri, Chromidotilapia kingsleyae, Chromidotilapia linkei, Chromidotilapia mamonekenei, Chromidotilapia melaniae, Chromidotilapia mrac, Chromidotilapia nana, Chromidotilapia regani, Chromidotilapia schoutedeni, Chrysichthys aluuensis, Chrysichthys ansorgii, Chrysichthys auratus, Chrysichthys bocagii, Chrysichthys brachynema, Chrysichthys brevibarbis, Chrysichthys cranchii, Chrysichthys dageti, Chrysichthys delhezi, Chrysichthys dendrophorus, Chrysichthys duttoni, Chrysichthys furcatus, Chrysichthys nigrodigitatus, Chrysichthys grandis, Chrysichthys graueri, Chrysichthys habereri, Chrysichthys helicophagus, Chrysichthys hildae, Chrysichthys johnelsi, Chrysichthys laticeps, Chrysichthys levequei, Chrysichthys longibarbis, Chrysichthys longidorsalis, Chrysichthys longipinnis, Chrysichthys mabusi, Chrysichthys macropterus, Chrysichthys maurus, Chrysichthys nyongensis)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((50.7597 -16.0878,50.8081 -15.9401,46.1382 -15.9401,46.1382 -24.9401,47.6434 -24.9401,49.8893 -18.4615,50.0065 -17.575,50.4326 -17.1196,50.4413 -16.4368,50.7597 -16.0878))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006504-250827131500795/datasets/export for details.
DOI: 10.22002/g26r2-zgc45
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-84/rawdata/</p>dH_LG_64step2_55.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-84/rawdata/dH_LG_64step2_55.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dyk9s-ywn75
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-30/rawdata/</p>dH_LG_64step2_18.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-30/rawdata/dH_LG_64step2_18.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9eakq-9qm26
title: M. xanthus pilA - pilY1.3-flag

description: Raw data files of M. xanthus pilA - pilY1.3-flag
description: <strong>Tilt Series Date:</strong> 2020-01-31</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Yi-Wei Chang, Anke Treuner-Lange, Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated 15 mL 1% CTT with 1mL previously frozen glycerol stock. . Incubated at 32C/225rpm for 2 days.</p>
<strong>Sample Preparation:</strong> Harvested, mixed 40uL cells with 10nm Au fiducials, plunge froze 4uL . (bf 6, bt 6s, d 1s)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-14/rawdata/</p>pilY13_2_019_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 5.7 GB  
        
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