DOI: 10.18150/p7veei
title: Juniper Berry Oil as a Functional Additive in Chitosan–Water Kefiran–Paramylon Porous Sponges: Structural, Physicochemical, and Protein Interaction Insights

description: 1. General Information- **Data Set Title**: Juniper Berry Oil as a Functional Additive in Chitosan–Water Kefiran–Paramylon Porous Sponges: Structural, Physicochemical, and Protein Interaction Insights- **Authors**: - Principal investigator and contact person: Dorota Chełminiak-Dudkiewicz, Nicolaus Copernicus University in Torun, e-mail: dorotachd@umk.pl.- **Date of data collection**: year 2025.- **Place of data collection**: Torun- **Keywords***: juniper oil; water kefiran; paramylon; biomaterials; biomedical applicationData language**: English- **Sources of funding**: National Science Center (NCN) competition: SONATA 18- UMO-2022/47/D/NZ7/01821.2. Overview of data and files:-**abbreviations**:CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P)CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w)CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w)CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)- **File List**:Fig.1.tiff: Graphic display of the main findings. Isolation of water kefiran (A) and scheme of obtaining CS/WK/P% sponges (B).Fig.2. tiff: Morphology characterization of the obtained sponges. SEM images (A), porosity (B), and 3D profile (C).Fig.3.tiff: Characterization of the CS/WK/P%JBO sponges. ATR-FTIR spectra (A) and adhesion force (B). Statistical significance: p &lt; 0.001 (**), p &lt; 0.0001 (***), p &lt; 0.00001 (****).Fig.4.tiff: Mechanical cyclic performance of sponges. Strain–stress curves corresponding to the 1st and 10th loading–unloading cycles (A), and maximum stress and hysteresis energy recorded for the 1st and 10th cycles (B).Fig.5.tiff: Properties of the sponges. Swelling rate (A), biodegradation rate (B), anti-inflammatory properties (C), and antioxidant properties (D). Statistical significance: ns (not significant), p &lt; 0.05 (*), p &lt; 0.01 (**), p &lt; 0.001 (***), p &lt; 0.0001 (****).Fig.6.tiff: Protein adsorption on the obtained sponges. Amount of the HSA (A) and fibrinogen (B) bound on the materials. Symbols #, *, and $ indicate p &lt; 0.05 when compared to CS/WK/P, CS/WK/P5%JBO, and CS/WK/P10%JBO, respectively.Raw data: adhesion glass.tab: The adhesive strength of the sponges on the glass surface was tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w).adhezja plastik.tab: The adhesive strength of the sponges on the plastic surface was tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w).adhezja pork.tab: The adhesive strength of the sponges on the pork surface was tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w).anti-inflammatory.txt: The raw data on the anti-inflammatory properties were determined by inhibiting the denaturation of bovine serum albumin (BSA) with the obtained materials. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)antioxidant.txt: The raw data on the anti-inflammatory properties were determined by the DPPH scavenging test. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)fibrinogen adsorption.txt: The fluorescence method was used to determine the adsorption of fibrinogen (FB) onto the materials. Fluorescence spectra were recorded at various time intervals at 25 C, ranging from 290 to 400 nm and 300 to 500 nm for HSA and Fb, respectively, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)guma adhesion.tab: The adhesive strength of the sponges on the gum surface was tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)HSA adsorption.txt: The fluorescence method was used to determine the adsorption of human serum albumin (HSA) onto the materials. Fluorescence spectra were recorded at various time intervals at 25 C, ranging from 290 to 400 nm and 300 to 500 nm for HSA and Fb, respectively, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)hysteresis.txt: Hysteresis behavior was evaluated through tensile loading–unloading experiments performed on sponge-like samples using a universal testing machine. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)metal adhesion.tab: The adhesive strength of the sponges on the metal surface was tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)swelling.txt: A swelling test was performed at pH 5.6 and pH 7.4. The samples were weighed, and the degree of swelling was calculated using a formula. The data was prepared using GraphPad Prism 10. Legend: CS/WK/P - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P); CS/WK/P5%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (5%v/w); CS/WK/P10%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (10%v/w); CS/WK/P20%JBO - sponge based on chitosan (CS), water kefiran (WK), and paramylon (P), enriched with juniper berry oil (JBO) (20%v/w)- **File formats**: tiff, tab, txt3. sharing and access information- **Licenses/Restrictions**: CC BY - Creative Commons Attribution 4.0. - **Related Publications**: https://doi.org/10.3390/ijms26115314