cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14285753.v1
title: Protein profile as a quality indicator of cryopreserved semen from Tambaqui Colossoma macropomum (Cuvier, 1818)

description: Abstract The aim of this study was to evaluate the association between proteins in the seminal plasma of tambaqui Colossoma macropomum (Cuvier, 1818) with seminal quality indicators after thawing. The semen was cryopreserved with a dilution based on BTS with 8% DMSO. A 200 µL sample of semen from each animal was diluted in 800 µL BTS, centrifuged at 800 rpm, and the supernatant was cryopreserved to further analyze of the protein profile of seminal plasma through one-dimensional electrophoresis (SDS-PAGE). After 15 days of cryopreservation, a cryopreserved semen straw was thawed to analyze both qualitative and quantitative parameters. When considering all collections, the SDS-PAGE identified 15 protein bands in the seminal plasma of tambaqui. When the interaction (presence or absence) between proteins observed in the seminal plasma and the post thawed spermatic parameters was evaluated, we observed a great influence of the presence of proteins on spermatic quality. A greater (P&lt;0.05) fertilization rate was observed with the presence of proteins 12, 34, 44, 85, and 90 kDa. Proteins in seminal plasma of tambaqui influenced the spermatic quality after thawing, and thus, they can be utilized as an indicator of sperm quality, especially the proteins with a molecular weight ≤ 50 kDa.
DOI: 10.6084/m9.figshare.14285752.v1
title: In vitro haemostatic efficacy of aqueous, methanol and ethanol plant extracts of three medicinal plant species in Palestine

description: Abstract The haemostatic efficacy of different extract types of Satureja thymbra L., Thymbra spicata L. (Lamiaceae) and Verbascum fruticulosum Post. (Scrophulariaceae) was evaluated in this study via the Prothrombin time (PT) and Activated partial thromboplastin time (aPTT) analysis. Aqueous, methanol and ethanol extracts of the examined plant species leaves were prepared to a final concentration 50 mg/mL. In vitro PT and aPTT assays were conducted on normal platelet poor plasma blood samples by a digital coagulation analyzer. The obtained results revealed anticoagulation activity of all investigated plant species with observed variations among them. The aqueous and ethanol extracts of T. spicata as well as the aqueous extract of S. thymbra prolonged PT values significantly (p &lt; 0.05). While, all V. fruticulosum extract types have had no significant effect on the PT values. The recorded aPTT data showed that all aqueous extracts have had a significant effect on the blood haemostasis as they increased aPTT values in all plant species under study. Out of which, both the ethanol and methanol extracts of T. spicata and methanol extract of S. thymbra showed similar effect. Of great concern, it was clearly noticed that the aqueous and ethanol extract of T. spicata and the aqueous extract of S. thymbra possess the strongest anticoagulation effect as they increased both PT and aPTT values significantly relative to the control (p &lt; 0.05). The variable anticoagulation bioactivity among the studied plant species could be referred to the various solvents degrees of solubility of different phyto-constituents. Thus, the efficacy of the plant species extracts evaluation as anticoagulants or coagulants were related to the plant species and to the solvent of extraction.
DOI: 10.6084/m9.figshare.14285750.v1
title: The influence of catch-and-release on mortality of Salminus brasiliensis (Cuvier, 1816)

description: Abstract Catch-and-release (CR) angling has been used to assist management programs seeking to promote the conservation and recovery of some recreational fish stocks by decreasing mortality from fishing. However, without appropriate fish handling, this activity may cause damage and injuries to individual fish, causing mortality. Therefore, CR should be allowed under some circumstances that ensure higher rates of survival to achieve the goals of the conservation measures. Thus, we experimentally tested the CR effects on dourado (Salminus brasiliensis) by comparing caught-and-released fish (CR fish) with uncaught fish (control fish) in a pond and estimating individual mortality rates related to seven air exposure times (AET: 120 s, 240 s, 360 s, 480 s, 600 s, 720 s and 840 s). We also determined a safe limit for fish handling and investigated the length and weight gain variations. We found that an increase in AET affects fish survival and that the limit of handling time for this species was 546 s; after this threshold, the individual probability of death exceeds 5%. The length and weight gain of CR fish did not differ significantly from the control fish. We believe that CR is efficient for S. brasiliensis because it does not significantly impair their organic growth and causes little or no mortality if handling is performed properly; thus, it could be employed to assist conservation programs.
DOI: 10.6084/m9.figshare.14285748.v1
title: Influence of the host diet on the performance of Doryctobracon areolatus (Hymenoptera: Braconidae)

description: Abstract Fitness in parasitoids is generally influenced by host quality. We evaluated the development parameters of Doryctobracon areolatus (Hymenoptera: Braconidae) parasitoid on Anastrepha fraterculus (Diptera: Tephritidae) larvae, reared in artificial diets with different protein sources. Larvae of A. fraterculus were reared in the diets based on 1) raw wheat germ (control); 2) whole rice flour; 3) corn flour; and, 4) whole wheat flour + soybean meal. The larvae were used for the development of D. areolatus, to evaluate the number of offspring/female, emergence rate, sex ratio, egg-adult period, adult weight posterior tibia length and longevity. Larvae of A. fraterculus reared in the diets based on wheat germ and corn flour obtained a greater number of offspring, with shorter duration of egg-adult period for males and females. In diets with rice flour and corn flour, A. fraterculus larvae generated adults of D. areolatus with greater weight, longer tibia length and higher sex ratio, whereas larvae reared in wheat flour + soybean meal diet had a lower sex ratio. The diets based on rice flour and corn are the most appropriate for multiplication of the parasitoid.
DOI: 10.6084/m9.figshare.14285745.v1
title: Verification of atmospheric plasma irradiation as an alternative control method for Tribolium castaneum (Herbst)

description: Abstract Radio frequency plasma, RF plasma, has been applied to a number of industrial applications. Laboratory experiments were conducted to determine the RF plasma potential in Tribolium castaneum (Herbst) control. Three treatment variables: (1) helium or argon plasmas; (2) two power levels (50 and 100 watt); and (3) different RF plasma exposure times (0, 20, 40, 60 and 90 seconds) were examined in this study. The susceptibility of T. castaneum to treatment varies as gas, power level, and the exposure time varies. A positive correlation was found between insect mortality percent in treating larvae as well as treated pupae and exposure time to RF plasma and also the power level of irradiation. The results revealed that no live insects were found after a 90 second, 100 watt helium RF plasma treatment. The survival percents of the adults were developed from treated larvae and treated pupae were significantly reduced by increasing of the exposure time or power level. Obtained data indicated that the treatment with RF plasma might be considered as an environmentally compatible or alternative supplement to the other control methods for stored product-pests management.
DOI: 10.6084/m9.figshare.14285744.v1
title: Assessment of interspecific interactions between the invasive red-claw crayfish (Cherax quadricarinatus) and the Mozambique tilapia (Oreochromis mossambicus)

description: Abstract The Australian red-claw crayfish, Cherax quadricarinatus, has been introduced for aquaculture purposes worldwide and consequently colonized natural environments, where it might cause ecosystem services losses or adversely affect native species and the local environment. This species was first found in Pequenos Libombos Reservoir in Maputo Province, Southern Mozambique in the late 2009 and is linked to reduction in tilapia fisheries. This study, conducted in 2015 under controlled conditions, aimed to assess the interspecific relationships between the Mozambique tilapia (Oreochromis mossambicus) and the alien crayfish. For both species, no significant differences in growth and survival rates were found between animals reared in the presence versus the absence of heterospecifics, indicating no direct deleterious interspecific effects. Behavioural observations revealed that fish and crayfish competed for shelter and food. Both species reduced the foraging in the presence of heterospecifics during feeding period, in the daytime. Crayfish seemed to have an advantage in competition for shelter, suggesting that they may interfere with tilapia sheltering activity and make tilapia vulnerable to predators in natural habitats.
DOI: 10.6084/m9.figshare.14285739.v1
title: Survival, development and reproduction of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) on kaolin-treated cotton

description: Abstract The mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a cotton pest widespread in several cotton growing regions of Brazil, particularly in the semi-arid region of southwestern Bahia. The impact of kaolin on survival, development and reproduction of P. solenopsis was evaluated in the laboratory. The experiment was developed in a completely randomized design with two treatments: immature or newly emerged adults of P. solenopsis sprayed with kaolin and fed with cotton leaf discs treated with kaolin suspension (with kaolin) (T1) and immature or newly emerged adults of P. solenopsis sprayed with distilled water and fed with cotton leaf discs treated with distilled water (without kaolin) (T2). The kaolin suspension shortens the life cycle, increases the reproductive potential and population growth of the cotton mealybug, P. solenopsis and, therefore, it should be used with caution on cotton plants in regions with a history of occurrence of this pest.
DOI: 10.6084/m9.figshare.14285733.v1
title: Physicochemical quality of and Escherichia coli resistance profiles in urban surface waters

description: Abstract Aquatic ecosystems of urban rivers are contaminated through waste disposal, which poses a public health problem. The objective of this research was to evaluate the quality of water used for recreation and public supply of six rivers in the city of Cascavel - Paraná, including Cascavel, Quati, Bezerra, Antas, Clarito and Amambay. Samples were collected every 4 months in 2017, and their physicochemical and microbiological parameters, as well as resistance profiles of strains of Escherichia coli to antimicrobials distributed by pharmacies of the primary healthcare network, were evaluated. Parameters such as water temperature, turbidity, total nitrogen, total phosphorus, total coliforms and thermotolerant coliforms showed significant differences. The allowed limit for thermotolerant coliforms, which was set by National Environment Council, Resolution 357/2005, was exceeded in all of the six analyzed rivers. It was determined that 48.1% of E. coli strains showed resistance to nine antimicrobial tested. The highest levels of resistance were found for ampicillin (27.7%), tetracycline (27.7%) and amoxicillin (24.0%). The results of this study contribute to the understanding of the hazards associated with the contamination of springs in urban centers with wastewater containing resistant bacteria. Therefore, recovery work is necessary in these areas because of the importance of these water sources for the entire western region of Paraná state.
DOI: 10.6084/m9.figshare.14285732.v1
title: Root-knot nematode, Meloidogyne javanica, in response to soil fertilization

description: Abstract Root-knot nematodes (RKNs) (Meloidogyne spp.) are well known disease problem causing major losses in vegetable crops. Although, chemical nematicides have been used as one of the primary means for controlling RKNs, reliance on these nematicides is associated with heavy costs and negative effects on human health and environment. In this research, the suppressing potential of 6 Iranian commercial fertilizers on RKNs was investigated in laboratory and greenhouse conditions as an alternative to reduce the use of chemical nematicides. For this purpose, M. javanica inoculum was thoroughly mixed with autoclaved sandy loam soil. Then, 6 fertilizers (biofertilizer, phosphate chemical fertilizer (phosphate), potassium chemical fertilizer (potassium), peat moss, vermicompost, and leaf mold) were added individually to the inoculated soil, according to the defined treatments. The nematicide Cadusafos was used as a positive control. A negative control was also included in the experiment (including no fertilizer and no nematicide). Four-leaf seedlings of the tomato (Super Chief cv.) were transferred to the pots filled using 2 kg of the treated soil. After 60 days, reproduction factor (RF), egg mass, and root galling of the nematode were recorded. The results showed that, fertilizer-treated soils had significantly (P≤0.05) lower root galling, egg mass, and RF compared to the negative control. After the nematicide treatment, the highest suppression capability on the RKN was obtained in treatments of phosphate, biofertilizer, potassium, vermicompost, peat moss, and leaf mold, respectively. It can be concluded that, replacing chemical nematicides with fertilizers may be considered as a successful nematode management in tomato fields.
DOI: 10.6084/m9.figshare.14285731.v1
title: Effect of oviposition by Bactrocera dorsalis on the antioxidant activity of orange juice

description: Abstract Among fruits and fruit products, oranges and orange juice are the most widely consumed worldwide. However, the effects of pest infestation of oranges on the quality of orange juice are not yet known. To evaluate the effect of the oriental fruit fly Bactrocera dorsalis on the antioxidant activity of orange juice, we measured changes in the vitamin C (Vc) concentration, total phenol content, and antioxidant activity of orange juice after the introduction of fruit fly eggs. Ten days after the eggs were introduced (larvae removed), the concentration of Vc in orange juice was 18.65 µg/mL, which was 9.16 µg/mL lower than that measured in healthy orange juice. In addition, the total phenol content decreased by 46.519 mg Gallic Acid Equivalents (GAE)/g to 9.748 mg GAE/g. Furthermore, the free-radical scavenging activity decreased from 22.297% to 5.393%. Correlation analysis indicated significant correlations between Vc concentration, total phenol content, and antioxidant activity of orange juice after B. dorsalis infestation. The decrease in Vc concentration, total phenol content and free-radical scavenging activity indicated that B. dorsalis changed the quality of orange juice by affecting the antioxidant activity of the juice after the oranges were infested.
DOI: 10.6084/m9.figshare.14285727.v1
title: A green approach for the treatment of dye and surfactant contaminated industrial wastewater

description: Abstract Synthetic dyes, particularly reactive and acid dyes, are commonly used in the textile industry because of their advantages as excellent color fastness and brightness. Also, surfactants are used for an increment of coloring success in the textile industry. One of the major problems concerning textile wastewater is the treatment of the effluents containing both dyes and surfactants. Biological treatment systems are recommended as useful, economic and eco-friendly methods for treatment of industrial wastewater. The purpose of this study was to investigate the binary removal of a textile dye and a surfactant by growing Aspergillus versicolor culture in molasses medium. The effect of dye and surfactant concentration on the removal of dye and surfactant was determined. This study resulted that 100% of the surfactant and dye molecules removed together with the formation of a dye-surfactant complex by fungus. It is concluded that binary removal systems are very efficient for industrial wastewater treatment.
DOI: 10.6084/m9.figshare.14285724.v1
title: Leaf application of chitosan and physiological evaluation of maize hybrids contrasting for drought tolerance under water restriction

description: Abstract It is a fact that the regions that cultivate the most maize crop do not have fully adequate technologies to measure productivity losses caused by irregularities in water availability. The objective of this study was to evaluate the physiological characteristics of maize hybrids tolerant (DKB 390) and sensitive (BRS 1030) to drought, at V5 growth stage and under water restriction, in order to understand the mechanisms involved in the induction of tolerance to drought by chitosan in contrasting maize genotypes. Plants were cultivated in pots at a greenhouse, and chitosan 100 ppm was applied by leaf spraying. The water restriction was imposed for 10 days and then leaf gaseous exchange and chlorophyll fluorescence were evaluated. The tolerant hybrid (DKB 390) showed higher photosynthesis, stomatal conductance, carboxylation efficiency, electron transport rate, and non-photochemical quenching when chitosan was used. Plants from tolerant genotype treated with chitosan were more tolerant to water stress because there were more responsive to the biopolymer.
DOI: 10.6084/m9.figshare.14285722.v1
title: Evaluation of the effects of sugarcane molasses as a carbon source for Ankistrodesmus gracilis and Haematococcus pluvialis (Chlorophyceae)

description: Abstract Two species of Chlorophyceae, Ankistrodesmus gracilis and Haemotococcus pluvialis, were used to compare and evaluate the effect of sugarcane molasses as a carbon source. Highest cell density in the two microalgae culture media was obtained in commercial culture media (CHU12 and WC). During exponential growth (day 1 to day 10), high cell density in H. pluvialis was detected for E. crassipes culture medium ranging between 0.4 x 105 cells mL-1 and 1.7 x 105 cells mL-1. Culture media were fundamental for growth under mixotrophic cultivation. Sugarcane molasses showed different results for the two microalgae with regard to growth performance, lipid and protein levels. Rates were high for H. pluvialis except lipid at the end of the experiment. In fact, A. gracilis presented almost double the lipid levels. Sugarcane molasses may be an alternative carbon source in laboratory conditions.
DOI: 10.6084/m9.figshare.14285720.v1
title: Assessment and incidence of fish associated bacterial pathogens at hatcheries of Azad Kashmir, Pakistan

description: Abstract Fish is the most indispensable source of proteins for individuals and have high nutritional value. On the other hand, the fish culturing raised issues of fish health due to close contact between the aquatic environment and the fish pathogens. So, the aim of the current study was to identify the bacterial pathogens and screen the injured Rainbow trout rearing in different trout hatcheries run under fisheries department of the government of Azad Jammu and Kashmir, Pakistan. Seven bacterial pathogens such as Shigella flexneri, Enterobacter amnigenus, Salmonella Typhimurium, Serratia odorifera, Pseudomonas aeruginosa, Streptococcus pyogenes, and Bacillus cereus were isolated and identified. Results revealed that the injury of fish specimens was due to overcrowding. Instead of rainbow coloration, specimens have darker black in color. The water of ponds was not clean and clear and such conditions was because of the greater quantity of feed thrown in the water. It was concluded that poor hygienic water condition and overloading allowed the opportunistic bacterial contaminations to succeed which cause a serious threat to hatcheries.
DOI: 10.6084/m9.figshare.14285718.v1
title: In vivo effect of essential oil of Mentha x villosa and its active compound against Schistosoma mansoni (Sambon, 1907)

description: Abstract Schistosomiasis treatment is dependent on a single drug, praziquantel (PZQ). The development of resistance of PZQ has drawn the attention of many researchers to alternative drugs. One viable and promising treatment is the study of medicinal plants as a new approach to the experimental treatment for Schistosomiasis. The present work aimed to evaluate in vivo antischistosomal activity of effect of Mentha x villosa Oil Essential (Mv-EO) and rotundifolone (ROT) against Schistosoma mansoni. Thirty-day-old female Swiss webster mice (Mus musculus) weighing 50 grams were used. Mice were infected with 80 cercariae of S. mansoni (BH strain) and orally administered Mv-EO (50, 100 and 200 mg/Kg) and ROT (35.9, 70.9 and 141.9 mg/Kg) at 45-days post infection for 5 consecutive days. All mice were euthanized 60 days after infection. Praziquantel was the positive control in the experiment. Doses of 200 mg/kg (Mv-EO) and ROT (141.9 mg/Kg) resulted in a significant reduction in fluke burden (72.44% and 74.48%, respectively). There was also marked reduction in liver, intestinal and faecal and changed oogram pattern, compared to infected untreated mice. Considering the results obtained, further biological studies are required in order to elucidate the mechanism of schistosomicidal action on against adult S. mansoni.
DOI: 10.6084/m9.figshare.14285715.v1
title: Pharmacological evaluation of the hydro-alcoholic extract of Campomanesia phaea fruits in rats

description: Abstract Campomanesia phaea (Myrtaceae), popularly known as cambuci, is one of several species of plants producing comestible fruits, largely used in human nutrition. Despite its consumption and economic potential, limited scientific research is available on the Campomanesia, especially those related to its therapeutic benefits. It is reported by traditional medicine the use of the plant in the treatment of different disorders, such as cardiovascular and nervous system disturbances. So, the aim of this study was to carry out the pharmacological evaluation of the hydro-alcoholic extract (HAE) of Campomanesia fruits in rats by screening consisting of tests: a) neuropharmacological observation, b) test on the cardiovascular system. The HAE, prepared from the extraction of fruits with water/ethanol, was concentrated and freeze-dried. Behavioral responses in rats were investigated in open field test and the cardiovascular actions were investigated by a register of indirect blood pressure and the register of spontaneous beating rate right atrium. The results revealed that HAE induced grooming, hypotension and bradycardia. So, this study identified an action on the central nervous system, represented by grooming, and a cardiovascular activity of Campomanesia. The hypotension, attributed in part to bradycardia, was not related to a cholinergic effect, discarding a possible cholinomimetic action of the plant that could justify both cardiovascular and central actions.