DOI: 10.5061/dryad.brv15dvkg
title: <em>Aedes sierrensis</em> - <em>Lambornella clarki</em> host - parasite interaction data: Field survey and temperature dependence laboratory experiments

description: Temperature is a fundamental driver of species interactions, including
 parasitism. Here, we use field surveys and lab experiments to characterize
 the temperature dependence of the interaction between the mosquito host
 Aedes sierrensis and its facultative ciliate parasite Lambornella clarki.
 Field survey data quantifying infection presence across a 1100 km
 geographic climate gradient are paired with long-term environmental
 temperature measurements from PRISM. PRISM temperature estimates are
 compared to direct measurements of tree hole habitat temperatures logged
 with iButtons. Lab experiments with six host populations from different
 climates across the study area describe the fundamental temperature
 sensitivity of the host - parasite interaction and underlying host and
 parasite traits. 
description: There are five data files. Two files contain
 field data for tree holes sampled in the <em>Lambornella
 clarki</em> infection survey. One of these files contains the
 infection data from the sampled juvenile <em>Aedes
 sierrensis</em> mosquitoes, plus temperature and precipitation data
 from PRISM and RADS-CHIRPS. The other file contains temperature data
 logged by iButtons placed within tree holes, plus temperature data from
 PRISM and infection presence data. Three files contain
 data from the laboratory temperature dependence experiments. One file
 contains data from the infection experiment, one file contains data from
 the <em>Lambornella clarki</em> free-living population growth
 experiment, and one file contains data from the mosquito melanization
 assay.
description: # *Aedes sierrensis* - *Lambornella clarki* host - parasite interaction
 data: Field survey and temperature dependence laboratory experiments
 Dataset DOI:
 [10.5061/dryad.brv15dvkg](https://doi.org/10.5061/dryad.brv15dvkg) ##
 Description of the data and file structure Field survey data were
 collected from sampled tree holes in California, Oregon, and Washington
 during the 2022 and 2023 rainy seasons. Ibutton field temperature data was
 logged during between 2022 and 2023 sampling visits. Climate and weather
 data are extracted from PRISM using Google Earth Engine. Data from
 experiments were generated from incubation of Aedes sierrensis hosts and
 Lambornella clarki parasites at different temperatures in the laboratory. 
 ### Files and variables #### File:
 TreeHolesTemperature_LambornellaFreeLivingExperiment_Ecol.csv
 **Description:** Data from the free-living Lambornella clarki population
 growth experiment. ##### Variables * Temperature: incubation temperature
 (ºC) * Tray ID: identifier for the 6-well plate in which the microcosm was
 housed * Well ID: identifier for the microcosm * day0 – starting number of
 Lambornella clarki cells per 100 uL * day1 – number of Lambornella clarki
 cells per 100 uL after one day of incubation * day2 – number of
 Lambornella clarki cells per 100 uL after two days of incubation * day3 –
 number of Lambornella clarki cells per 100 uL after three days of
 incubation * day4 – number of Lambornella clarki cells per 100 uL after
 four days of incubation * day5 – number of Lambornella clarki cells per
 100 uL after five days of incubation * replicate – replicate number for
 the temperature treatment #### File:
 TreeHolesTemperature_MelanizationAssay_Ecol.csv **Description:** Data from
 the melanization assay experiment. ##### Variables * temperature –
 incubation temperature (ºC) * tray – identifier for the 6-well plate in
 which the microcosm was housed * well – identifier for the microcosm *
 start.date – date on which the melanization response was stimulated * time
 – time at which the melanization response was stimulated * check.date –
 date on which the melanization response was assessed * check.time – time
 at which the melanization response was assessed * num.alive – number of
 larvae alive at the time of the melanization assessment (out of 5) *
 num.melanized – number of larvae with a melanization spot at the puncture
 site #### File: TreeHoles_2022InfectionTemperatureExperiment_Ecol.csv
 **Description:** Data from the infection experiment. “NA” represents that
 the field was not applicable, e.g., num.infections was NA when there were
 no surviving larvae left to inspect for infections ##### Variables *
 temperature – incubation temperature (ºC) * tray – identifier for the
 6-well plate in which the microcosm was housed * population – host
 population identifier * well – identifier for the microcosm * climate –
 climate category for the host population * check.date – date of the
 microscope check * check.day – day of the experiment on which the
 microscope check took place * check.num – experimental checkpoint number *
 num.alive – number of juvenile mosquitoes alive * num.infected – number of
 ciliate infections observed * num.melanized – number of larvae with
 melanization spots * tot.melanized – cumulative larvae with melanization
 spots * tot.infected – cumulative larvae with ciliate infections *
 tot.dead – cumulative mosquito host deaths * deaths.infected – cumulative
 deaths of infected hosts * start.num – starting number of mosquito larvae
 in the microcosm * last.check – binary, 1 indicates that the row contains
 the final observation data for the microcosm * new.deaths.infected - count
 of new infection mortalities observed for the checkpoint * lambo.count -
 density of free-living ciliates per 300 uL in the microcosm #### File:
 TreeHoles_FieldSurvey_InfectionData_Ecol.csv **Description:**  #####
 Variables * treehole.id – identifier for each sampled tree hole *
 Date.of.Sampling – date of sample collection * num.checked – number of
 juvenile mosquitoes from the sample that were checked for ciliate
 infection and for melanization spots * num.infected – number of infected
 mosquitoes observed in the sample * num.melanized – number of mosquitoes
 with melanization spots observed in the sample * th.infected – binary, 1
 indicates infection presence in the sample * th.melanized – binary, 1
 indicates melanization spot presence in the sample * lat – tree hole
 latitude (º) * long – tree hole longitude (º) 
 
 #### File: TreeHoles_Ibuttons_Infection_Ecol.csv **Description:** Data
 from iButton temperature loggers placed within tree holes ##### Variables
 * treehole.id – identifier for the tree hole in which the iButton was
 placed * date – date of the temperature data * placementdate – date of
 iButton placement within the tree hole * finaldate – date of iButton
 removal from the tree hole * day_mean_temp_ib – the mean temperature
 recorded by the iButton on the date (ºC) * infected – binary, 1 indicates
 that infected mosquitoes were collected from the tree hole at least once
 during the survey #### File: TreeHoles_SampleDates_LatLon_Ecol.csv
 **Description:** gps coordinates and sampling dates for the infection
 field survey, for use in Google Earth Engine with
 "GEE_ExtractPRISM_FieldSurvey_Ecol.rtf" ##### Variables *
 latitude: surveyed tree hole latitude (º) * longitude: surveyed tree hole
 longitude (º) * sampledate: date on which tree hole was sampled *
 surveyyear: year of sampling * treeholeid: identifier for the sampled tree
 hole #### File: TreeHoles_ibuttons_forGEE_Ecol.csv
 **Description:** locations and dates for iButton logs of temperatures
 within tree hole habitats, for use in Google Earth Engine with
 "GEE_ExtractPRISM_iButtons_Ecol.rtf" ##### Variables *
 finaldate: date on which iButton was removed from the tree hole * ibutton:
 identifier number for the iButton * latitude: latitude of the tree hole in
 which the iButton was placed (º) * longitude: longitude of the tree hole
 in which the iButton was placed (º) * placementdate: date on which the
 iButton was placed inside the tree hole * treeholeid: identifier for the
 tree hole ## Code/software **File:
 TreeHoles_iButtonsPRISM_comparison_Ecol.R** **Description:** R script for
 analyzing the paired field iButton and PRISM temperature data **File:
 TreeHoles_TPCs_OverallInteraction_Ecol.R** **Description:** R script fits
 and plots thermal performance curves from the infection experiment for the
 data combined across all host populations; for the free-living Lambornella
 populatoin growth rate experiment; and for the melanization assay
 experiment  **File: TreeHoles_InfectionExperiment_PopulationTPCs_Ecol.R**
 **Description:** Fits and plots thermal performance curves from the
 infection experiment separately for each population **File:
 TreeHolesTemperature_InfectionExperimentTimeline_Ecol.R**
 **Description:** time series plots for the infection experiment **File:
 GEE_ExtractPRISM_FieldSurvey_Ecol.rtf** **Description:** Google Earth
 Engine script for extracting PRISM temperature and precipitation data used
 in the field survey analysis **File:
 TreeHoles_InfectionFieldSurvey_Analysis_Final_Ecol.R**
 **Description:** field survey infection data analysis **File:
 GEE_ExtractPRISM_iButtons_Ecol.rtf** **Description:** Google Earth Engine
 script for extracting PRISM daily mean temperature estimates for tree
 holes with iButton daily temperature time series **File:
 TreeHoles_Ibuttons_PRISM_Comparison_Ecol.R** **Description:** comparison
 of iButton and PRISM temperature data ## Access information Other publicly
 accessible locations of the data: * Extracted PRISM data used in the
 analyses are available on
 Github: [https://github.com/johannah-farner/TreeHoles_Ecol](https://github.com/johannah-farner/TreeHoles_Ecol)