DOI: 10.5061/dryad.k90h35k
title: Data from: Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity

description: Antarctic notothenioid fishes are highly stenothermal, yet their tolerance
 for warming is species-dependent. Because a body of literature points to
 the loss of cardiac function as underlying thermal limits in ectothermic
 animals, we investigated potential relationships among properties of
 ventricular mitochondrial membranes in notothenioids with known
 differences in both cardiac mitochondrial metabolism and organismal
 thermal tolerance. Fluidity of mitochondrial membranes was quantified by
 fluorescence depolarization for the white-blooded Chaenocephalus aceratus
 and the red-blooded Notothenia coriiceps. In these same membranes, lipid
 compositions and products of lipid peroxidation, the latter of which can
 disrupt membrane order, were analyzed in both species and in a second
 icefish, Pseudochaenichthys georgianus. Mitochondrial membranes from C.
 aceratus were significantly more fluid than those of the more
 thermotolerant species N. coriiceps (P &lt; .0001). Consistent with
 this, ratios of total phosphatidylethanolamine (PE) to total
 phosphatidylcholine (PC) were lower in membranes from both species of
 icefishes, compared to those of N. coriiceps (P &lt; .05). However,
 membranes of N. coriiceps displayed a greater unsaturation index
 (P &lt; .0001). No differences among species were found in membrane
 products of lipid peroxidation. With rising temperatures, greater contents
 of PC in mitochondrial membranes from ventricles of icefishes are likely
 to promote membrane hyperfluidization at a lower temperature than for
 cardiac mitochondrial membranes from the red-blooded notothenioid. We
 propose that physical and chemical properties of the mitochondrial
 membranes may contribute to some of the observed differences in thermal
 sensitivity of physiological function among these species.
description: Biederman et al (2019)
 2009 vs 2015 comparisonPolar lipid composition
 of mitochondrial membranes prepared from cardiac ventricle of
 Chaenocephalus aceratus and Notothenia coriiceps. Measurements were
 performed in animals collected in the region of the Western Antarctic
 Peninsula during the austral fall seasons of 2009 and
 2015.Biederman et al (2019)
 cardiolipinCardiolipin contents of
 mitochondrial membranes prepared from cardiac ventricle of Chaenocephalus
 aceratus, Notothenia coriiceps, and Pseudochaenichthys georgianus.
 Measurements were performed in animals collected in the region of the
 Western Antarctic Peninsula during the austral fall season of
 2015.Biederman et al (2019)
 lipid peroxidationLipid peroxidation of
 mitochondrial membranes prepared from cardiac ventricle of Chaenocephalus
 aceratus, Notothenia coriiceps, and Pseudochaenichthys georgianus.
 Measurements were performed in animals collected in the region of the
 Western Antarctic Peninsula during the austral fall season of
 2015.Biederman et al (2019)
 membrane fluidityFluidity of
 mitochondrial membranes prepared from cardiac ventricle of Chaenocephalus
 aceratus, Notothenia coriiceps, and Pseudochaenichthys georgianus.
 Measurements were performed in animals collected in the region of the
 Western Antarctic Peninsula during the austral fall season of
 2015.Biederman et al (2019)
 polar lipid profilePolar lipid profile of
 mitochondrial membranes prepared from cardiac ventricle of Chaenocephalus
 aceratus, Notothenia coriiceps, and Pseudochaenichthys georgianus.
 Measurements were performed in animals collected in the region of the
 Western Antarctic Peninsula during the austral fall season of
 2015.Biederman et al (2019)
 sample ID numbersSample IDs and thermal
 treatment information of mitochondrial membranes prepared from cardiac
 ventricle of Chaenocephalus aceratus, Notothenia coriiceps, and
 Pseudochaenichthys georgianus. Measurements were performed in animals
 collected in the region of the Western Antarctic Peninsula during the
 austral fall season of 2015.