cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.3370186
title: Distribution and accumulation of 10 nm silver nanoparticles in maternal tissues and visceral yolk sac of pregnant mice, and a potential effect on embryo growth

description: We examined the distribution of silver in pregnant mice and embryos/fetuses following intravenous injections of 10 nm silver nanoparticles (AgNPs) or soluble silver nitrate (AgNO<sub>3</sub>) at dose levels of 0 (citrate buffer control) or 66 µg Ag/mouse to pregnant mice on gestation days (GDs) 7, 8 and 9. Selected maternal tissues and all embryos/fetuses from control, AgNP- and AgNO<sub>3</sub>-treated groups on GD10 and control and AgNP-treated groups on GD16 were processed for the measurement of silver concentrations, intracellular AgNP localization, histopathology and gross examination of tissue morphology. Inductively-coupled plasma mass spectrometry revealed silver in all examined tissues following either AgNP or AgNO<sub>3</sub> treatment, with highest concentrations of silver in maternal liver, spleen and visceral yolk sac (VYS), and lowest concentrations in embryos/fetuses. For VYS, mean silver concentration following AgNO<sub>3</sub> treatment (4.87 ng Ag/mg tissue) was approximately two-fold that following AgNP treatment (2.31 ng Ag/mg tissue); for all other tissues examined, mean silver concentrations following either AgNP or AgNO<sub>3</sub> treatment were not significantly different from each other (e.g. 2.57 or 2.84 ng Ag/mg tissue in maternal liver and 1.61 or 2.50 ng Ag/mg tissue in maternal spleen following AgNP or AgNO<sub>3</sub> treatment, respectively). Hyperspectral imaging revealed AgNP aggregates in maternal liver, kidney, spleen and VYS from AgNP-treated mice, but not AgNO<sub>3</sub>-treated mice. Additionally, one or more embryos collected on GD10 from eight of ten AgNP-treated mice appeared small for their age (i.e. Theiler stage 13 [GD8.5] or younger). In the control group (<i>N</i> = 11), this effect was seen in embryos from only one mouse. In conclusion, intravenous injection of 10 nm AgNPs to pregnant mice resulted in notable silver accumulation in maternal liver, spleen and VYS, and may have affected embryonic growth. Silver accumulation in embryos/fetuses was negligible.
DOI: 10.6084/m9.figshare.3370186.v1
title: Distribution and accumulation of 10 nm silver nanoparticles in maternal tissues and visceral yolk sac of pregnant mice, and a potential effect on embryo growth

description: We examined the distribution of silver in pregnant mice and embryos/fetuses following intravenous injections of 10 nm silver nanoparticles (AgNPs) or soluble silver nitrate (AgNO<sub>3</sub>) at dose levels of 0 (citrate buffer control) or 66 µg Ag/mouse to pregnant mice on gestation days (GDs) 7, 8 and 9. Selected maternal tissues and all embryos/fetuses from control, AgNP- and AgNO<sub>3</sub>-treated groups on GD10 and control and AgNP-treated groups on GD16 were processed for the measurement of silver concentrations, intracellular AgNP localization, histopathology and gross examination of tissue morphology. Inductively-coupled plasma mass spectrometry revealed silver in all examined tissues following either AgNP or AgNO<sub>3</sub> treatment, with highest concentrations of silver in maternal liver, spleen and visceral yolk sac (VYS), and lowest concentrations in embryos/fetuses. For VYS, mean silver concentration following AgNO<sub>3</sub> treatment (4.87 ng Ag/mg tissue) was approximately two-fold that following AgNP treatment (2.31 ng Ag/mg tissue); for all other tissues examined, mean silver concentrations following either AgNP or AgNO<sub>3</sub> treatment were not significantly different from each other (e.g. 2.57 or 2.84 ng Ag/mg tissue in maternal liver and 1.61 or 2.50 ng Ag/mg tissue in maternal spleen following AgNP or AgNO<sub>3</sub> treatment, respectively). Hyperspectral imaging revealed AgNP aggregates in maternal liver, kidney, spleen and VYS from AgNP-treated mice, but not AgNO<sub>3</sub>-treated mice. Additionally, one or more embryos collected on GD10 from eight of ten AgNP-treated mice appeared small for their age (i.e. Theiler stage 13 [GD8.5] or younger). In the control group (<i>N</i> = 11), this effect was seen in embryos from only one mouse. In conclusion, intravenous injection of 10 nm AgNPs to pregnant mice resulted in notable silver accumulation in maternal liver, spleen and VYS, and may have affected embryonic growth. Silver accumulation in embryos/fetuses was negligible.
DOI: 10.6084/m9.figshare.3370177
title: The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells

description: Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co<sub>0.5</sub>Zn<sub>0.5</sub>Fe<sub>2</sub>O<sub>4+γ</sub> [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F<sub>2t</sub>-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
DOI: 10.6084/m9.figshare.3370177.v1
title: The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells

description: Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co<sub>0.5</sub>Zn<sub>0.5</sub>Fe<sub>2</sub>O<sub>4+γ</sub> [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F<sub>2t</sub>-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
DOI: 10.6084/m9.figshare.3370171
title: Suppression of basal and carbon nanotube-induced oxidative stress, inflammation and fibrosis in mouse lungs by Nrf2

description: The lungs are susceptible to oxidative damage by inhaled pathogenic agents, including multi-walled carbon nanotubes (MWCNT). The nuclear factor erythroid 2-related factor 2 (Nrf2) has been implicated in regulating the body’s defense against oxidative stress. Here, we analyzed the function of Nrf2 in the lungs. Under a basal condition, Nrf2 knockout (KO) mice showed apparent pulmonary infiltration of granulocytes, macrophages and B and T lymphocytes, and elevated deposition of collagen fibers. Exposure to MWCNT (XNRI MWNT-7, Mitsui, Tokyo, Japan) by pharyngeal aspiration elicited rapid inflammatory and fibrotic responses in a dose (0, 5, 20 and 40 μg) and time (1, 3, 7 and 14 d)-dependent manner. The responses reached peak levels on day 7 post-exposure to 40 μg MWCNT, evidenced by massive inflammatory infiltration and formation of inflammatory and fibrotic foci, which were more evident in Nrf2 KO than wild-type (WT) lungs. At the molecular level, Nrf2 protein was detected at a low level under a basal condition, and was dramatically increased by MWCNT in WT, but not Nrf2 KO, lungs. Activation of Nrf2 was inversely correlated with induced expression of fibrosis marker genes and profibrotic cytokines. Furthermore, the levels of ROS and oxidative stress were remarkably higher in Nrf2 KO than WT lungs under a physiological condition, and were dramatically increased by MWCNT, with the increase significantly more striking in KO lungs. The findings reveal that Nrf2 plays an important role in suppressing the basal and MWCNT-induced oxidant production, inflammation and fibrosis in the lungs, thereby protecting against MWCNT lung toxicity.
DOI: 10.6084/m9.figshare.3370171.v1
title: Suppression of basal and carbon nanotube-induced oxidative stress, inflammation and fibrosis in mouse lungs by Nrf2

description: The lungs are susceptible to oxidative damage by inhaled pathogenic agents, including multi-walled carbon nanotubes (MWCNT). The nuclear factor erythroid 2-related factor 2 (Nrf2) has been implicated in regulating the body’s defense against oxidative stress. Here, we analyzed the function of Nrf2 in the lungs. Under a basal condition, Nrf2 knockout (KO) mice showed apparent pulmonary infiltration of granulocytes, macrophages and B and T lymphocytes, and elevated deposition of collagen fibers. Exposure to MWCNT (XNRI MWNT-7, Mitsui, Tokyo, Japan) by pharyngeal aspiration elicited rapid inflammatory and fibrotic responses in a dose (0, 5, 20 and 40 μg) and time (1, 3, 7 and 14 d)-dependent manner. The responses reached peak levels on day 7 post-exposure to 40 μg MWCNT, evidenced by massive inflammatory infiltration and formation of inflammatory and fibrotic foci, which were more evident in Nrf2 KO than wild-type (WT) lungs. At the molecular level, Nrf2 protein was detected at a low level under a basal condition, and was dramatically increased by MWCNT in WT, but not Nrf2 KO, lungs. Activation of Nrf2 was inversely correlated with induced expression of fibrosis marker genes and profibrotic cytokines. Furthermore, the levels of ROS and oxidative stress were remarkably higher in Nrf2 KO than WT lungs under a physiological condition, and were dramatically increased by MWCNT, with the increase significantly more striking in KO lungs. The findings reveal that Nrf2 plays an important role in suppressing the basal and MWCNT-induced oxidant production, inflammation and fibrosis in the lungs, thereby protecting against MWCNT lung toxicity.
DOI: 10.6084/m9.figshare.3370144
title: Input collection

description: This folder is including the input data necessary to fully replicate the generation process of the enviroGRIDS scenarios described in the Future Land-Use change scenarios for the Black Sea catchment [doi:10.1016/j.envsci.2014.02.008]
DOI: 10.6084/m9.figshare.3370144.v1
title: Input collection

description: This folder is including the input data necessary to fully replicate the generation process of the enviroGRIDS scenarios described in the Future Land-Use change scenarios for the Black Sea catchment [doi:10.1016/j.envsci.2014.02.008]
DOI: 10.6084/m9.figshare.3370135
title: Explanatory document

description: his document gives a general overview on the input data used to generate the land-use change scenarios for the Black Sea catchment<br>
DOI: 10.6084/m9.figshare.3370135.v1
title: Explanatory document

description: his document gives a general overview on the input data used to generate the land-use change scenarios for the Black Sea catchment<br>
DOI: 10.6084/m9.figshare.3370033
title: Fixed and moored bodies using SPH with the Froude Krylov approximation

description: Tecplot packaged layout files containing data associated with manuscript "Fixed and moored bodies in steep and breaking waves using SPH with the Froude Krylov approximation", SJ Lind, PK Stansby, BD Rogers, J. Ocean Engineering and Marine Energy, 2016
DOI: 10.6084/m9.figshare.3370033.v2
title: Fixed and moored bodies using SPH with the Froude Krylov approximation

description: Tecplot packaged layout files containing data associated with manuscript "Fixed and moored bodies in steep and breaking waves using SPH with the Froude Krylov approximation", SJ Lind, PK Stansby, BD Rogers, J. Ocean Engineering and Marine Energy, 2016
DOI: 10.6084/m9.figshare.3370033.v1
title: F16-in-prep.lpk

description: Tecplot packaged layout files containing data associated with manuscript "Fixed and moored bodies in steep and breaking waves using SPH with the Froude Krylov approximation", SJ Lind, PK Stansby, BD Rogers, J. Ocean Engineering and Marine Energy, 2016
DOI: 10.6084/m9.figshare.3369994
title: GEHM_bams.tar.gz

description: Raw sequence files for all samples included in the Gut microbiota associated with infant weight-for-length in a longitudinal cohort of healthy, breastfed infants manuscript
DOI: 10.6084/m9.figshare.3369994.v1
title: GEHM_bams.tar.gz

description: Raw sequence files for all samples included in the Gut microbiota associated with infant weight-for-length in a longitudinal cohort of healthy, breastfed infants manuscript
DOI: 10.6084/m9.figshare.3369985
title: BoxC - Copulation Predator

description: Videos measuring copulation occurrence, latency and duration of 2 day old virgin <i>Drosophila melanogaster</i> females / males under threat of predation from zebra jumping spiders. Trials from April 11-16th 2016Note: BoxA is also with predator, but different recording Bin
DOI: 10.6084/m9.figshare.3369985.v2
title: BoxC - Copulation Predator

description: Videos measuring copulation occurrence, latency and duration of 2 day old virgin <i>Drosophila melanogaster</i> females / males under threat of predation from zebra jumping spiders. Trials from April 11-16th 2016Note: BoxA is also with predator, but different recording Bin
DOI: 10.6084/m9.figshare.3369985.v1
title: BoxC - Copulation Predator

description: Videos measuring copulation occurrence, latency and duration of 2 day old virgin <i>Drosophila melanogaster</i> females / males under threat of predation from zebra jumping spiders. Trials from April 11-16th 2016<br>
DOI: 10.6084/m9.figshare.3369952
title: Shupert, Lindsay Anne. 2016. INTEGRATIVE MANAGEMENT OF LESPEDEZA CUNEATA IN PRAIRIE RESTORATIONS. MS Thesis. Southern Illinois University Carbondale.

description: Background: <i>Lespedeza cuneata</i> is a perennial invasive legume native to Asia that is common across grasslands in the eastern half of the United States and parts of Canada. The competitive nature and limited palatability of <i>L. cuneata</i> for grazers in the tallgrass prairie has made it an undesirable and invasive weed. Objectives: This study was conducted to investigate an invasive species management plan for restored grasslands and prairie areas. Methods: Study plots (n=144) for experiment 1 (summer treatment) were established at Crab Orchard Wildlife Refuge in Jackson County Illinois in August of 2012 and were situated to contain infestations of the target species. Two herbicides, in five concentrations were applied in August of 2012. A native seed mix was added to a portion of the summer plots later, in February of 2013. These treatments were assessed in June and August of 2013 to determine herbicide effects on stem count and percent cover of <i>L. cuneata</i>, along with the composition and percent cover of other species present. Spring herbicide applications (n=18) for experiment 2 were established, assessed for <i>L. cuneata </i>cover and density, and treated with two herbicide treatments in May of 2013. Spring treatments were identical to existing plots and were re-assessed in August 2013 to determine species composition and the abundance of <i>L. cuneata</i>. Seed was not added to these plots. Analysis: Mixed Model analysis by site was performed to test the effect of herbicide and seeding treatments on <i>L. cuneata</i> for both experiments 1 &2. The summer plots were also tested for effects on other plant groups. A<b> </b>repeated measures PERMANOVA along with ANOSIM and PERMADISP was performed to assess community composition response to seeding and herbicide treatments in the summer plots. Results: Abundance of <i>L. cuneata</i> was reduced 67 to &lt;90% following herbicide treatment in Experiments 1 and 2. There were no differences in the effectiveness of herbicide treatments in in Experiment 1, sites 1 &3, and in Experiment 2. Supplemental seeding in the summer plots did not reduce the abundance of <i>L. cuneata</i> consistently across sites. Conclusion: Considerable short-term control of the target species was achieved in both the summer and spring plots, providing a window of opportunity for the imposition of additional methods of control. However, data from the summer plots suggest recovery of <i>L. cuneata</i> some sites, indicating further control would be necessary, with potentially the addition of other control methods or site alterations.
DOI: 10.6084/m9.figshare.3369901
title: PLoSOne:Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model

description: The compressed file contains the dataset and the source-code for the paper: Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model<br>
DOI: 10.6084/m9.figshare.3369901.v1
title: PLoSOne:Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model

description: The compressed file contains the dataset and the source-code for the paper: Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model<br>
DOI: 10.6084/m9.figshare.3369901.v3
title: PLoSOne:Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model

description: The compressed file contains the dataset and the source-code for the paper: Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model<br>
DOI: 10.6084/m9.figshare.3369901.v2
title: Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model

description: The compressed file contains the dataset and the source-code for the paper: Prediction of Pathological Stage in Patients with Prostate Cancer: A Neuro-Fuzzy Model<br>Georgina Cosma, Giovanni Acampora, David Brown, Robert C. Rees,Masood Khan, A. Graham Pockley. PLoS ONE, 2016.
DOI: 10.6084/m9.figshare.3369832
title: MMGEPS

description: MMGEP with gene symbols.
DOI: 10.6084/m9.figshare.3369832.v1
title: MMGEPS

description: MMGEP with gene symbols.
DOI: 10.6084/m9.figshare.3369790
title: Pacing during an ultramarathon running event in hilly terrain

description: <b>Purpose.</b> The dynamics of speed selection as a function of distance, or pacing, are used in recreational, competitive, and scientific research situations as an indirect measure of the psycho-physiological status of an individual. The purpose of this study was to determine pacing on level, uphill and downhill sections of participants in a long (~173 km) ultramarathon performed on trails in hilly terrain. <b>Methods.</b> Fifteen ultramarathon runners competed in a ~173 km event (five finished at ~103 km) carrying a Global-Positioning System (GPS) device. Using the GPS data, we determined the speed, relative to average total speed, in level (LEV), uphill (UH) and downhill (DH) gradient categories as a function of total distance, as well as the correlation between overall performance and speed variability, speed loss, and total time stopped. <b>Results.</b> There were no significant differences in normality, variances or means in the relative speed in 173-km and 103-km participants. Relative speed decreased in LEV, UH and DH. The main component of speed loss occurred between 5% and 50% of the event distance in LEV, and between 5% and 95% in UH and DH. There were no significant correlations between overall performance and speed loss, the variability of speed, or total time stopped. <b>Conclusions.</b> Positive pacing was observed at all gradients, with the main component of speed loss occurring earlier (mixed pacing) in LEV compared to UH and DH. A speed reserve (increased speed in the last section) was observed in LEV and UH. The decrease in speed and variability of speed were more important in LEV and DH than in UH. The absence of a significant correlation between overall performance and descriptors of pacing is novel and indicates that pacing in ultramarathons in trails and hilly terrain differs to other types of running events.
DOI: 10.6084/m9.figshare.3369745
title: The <i>CELSR1</i> polymorphisms rs6007897 and rs4044210 are associated with ischaemic stroke in Chinese Han population

description: <i>Background</i>: Recently, CELSR1 was identified by genome-wide association studies (GWAS) as a susceptibility gene for ischaemic stroke (IS) in Japanese individuals. <i>Aim</i>: The goal was to examine whether CELSR1 variants are associated with IS in the Chinese Han population. <i>Subjects and methods</i>: This study genotyped two single nucleotide polymorphisms (SNPs) of CELSR1, rs6007897 and rs4044210, in a Chinese sample of 569 IS cases and 581 controls and assessed their genotype and allele associations with IS. <i>Results</i>: The results showed that rs6007897 and rs4044210 variants of CELSR1 were significantly (<i>p</i> p <i>Conclusion</i>: Taken together, the present study has proven for the first time that CELSR1 is a susceptibility gene for IS in the Chinese Han population, especially for LAA.
DOI: 10.6084/m9.figshare.3369745.v2
title: The <i>CELSR1</i> polymorphisms rs6007897 and rs4044210 are associated with ischaemic stroke in Chinese Han population

description: <i>Background</i>: Recently, CELSR1 was identified by genome-wide association studies (GWAS) as a susceptibility gene for ischaemic stroke (IS) in Japanese individuals. <i>Aim</i>: The goal was to examine whether CELSR1 variants are associated with IS in the Chinese Han population. <i>Subjects and methods</i>: This study genotyped two single nucleotide polymorphisms (SNPs) of CELSR1, rs6007897 and rs4044210, in a Chinese sample of 569 IS cases and 581 controls and assessed their genotype and allele associations with IS. <i>Results</i>: The results showed that rs6007897 and rs4044210 variants of CELSR1 were significantly (<i>p</i> p <i>Conclusion</i>: Taken together, the present study has proven for the first time that CELSR1 is a susceptibility gene for IS in the Chinese Han population, especially for LAA.