cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7899017.v1
title: Development of Imagination and Creativity Through the Game Design by Children in Inclusive School

description: ABSTRACT: This paper examines the development of imagination and creativity in children with and without disabilities through game design. The study is based on Vygotsky’s theories related to conceptual elaboration, imagination and creativity in childhood, and in advanced studies in game design with children. The Design-Based Research (DBR) was adopted as a methodological approach, configuring qualitative and collaborative research for game creation. Encounters were held weekly at a public school in the city of Itajaí, Santa Catarina, Brazil, for six months, involving a group of four nine-year-old children, including two with intellectual disabilities, and researchers and undergraduate students in the areas of computing, design, and education. The aim was to demonstrate the potential of the collective, creative and shared approach of game creation for the intellectual development of children in contexts of school inclusion. As a result, the framework “I’ve made my game” was defined for the development of games in school contexts. The analysis of the research results reveals the empowerment of the children involved in face of the proposed challenges, the intellectual operation at complex levels, the protagonism in the construction of solutions and the valorization of individual differences, favored by the collective and collaborative activities experienced in the process of creating digital games.
DOI: 10.6084/m9.figshare.7899020.v1
title: Evaluation of incidence and histolopathological findings of soft tissue sarcomas in genitourinary tract: Uludag university experience

description: ABSTRACT Purpose: In this study we aimed to review urological soft tissue sarcomas of genitourinary tract that were diagnosed in our institution and their prognostic factors for survival. Materials and Methods: The clinical and pathological records of 31 patients who had diagnosis of soft tissue sarcomas primarily originating from the genitourinary tract between 2005-2011 were reviewed. Results: The most common site was kidney (17 cases, 54.8%), and most common diagnosis was leiomyosarcoma (11 cases, 35.4%). A total of 24 patients (77.4%) had surgical excision. The surgical margins were positive in 7 patients who presented with local recurrence after primary resection. Twelve patients developed metastatic disease. During follow-up (range 9-70 month), 26 of the 31 patients (88.9%) were alive. Significant survival differences were found according to histological type (p: 0.001), with lower survival rates for malignant fibrous histiocytoma. The tumor size, the presence of metastasis at the time of diagnosis and tumor localization were not statistically significant for overall survival. Conclusions: In our series, prostate sarcomas, paratesticular rhabdomyosarcoma and malignant fibrous histiocytoma had poor prognosis, especially in patients presenting with metastatic disease.
DOI: 10.6084/m9.figshare.7899005
title: In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae

description: The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The chemical compositions of the active extracts were investigated using high performance liquid chromatography. Eleven extracts obtained from five Annonaceae plant species were active and were particularly effective against the UA251, NCI-470 lung, HT-29, NCI/ADR, and K-562 cell lines with growth inhibition (GI50) values of 0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL, respectively. In addition, the Annona crassiflora and A. coriacea seed extracts were the most active among the tested extracts and the most effective against the tumor cell lines, with GI50 values below 8.90 µg/mL. The A. cacans extract displayed the lowest activity. Based on the microplate assay, the percent AchE inhibition of the extracts ranged from 12 to 52%, and the A. coriacea seed extract resulted in the greatest inhibition (52%). Caffeic acid, sinapic acid, and rutin were present at higher concentrations in the A. crassiflora seed samples. The A. coriacea seeds contained ferulic and sinapic acid. Overall, the results indicated that A. crassiflora and A. coriacea extracts have antiproliferative and anticholinesterase properties, which opens up new possibilities for alternative pharmacotherapy drugs.
DOI: 10.6084/m9.figshare.7899011.v1
title: Association between BRAF (V600E) mutation and clinicopathological features of papillary thyroid carcinoma: a Brazilian single-centre case series

description: ABSTRACT Objectives: We aimed to investigate the prevalence of the BRAF (V600E) mutation in consecutive cases of papillary thyroid carcinoma (PTC) in patients diagnosed and treated at the Hospital Sao Rafael (Salvador, BA, Brazil) and evaluate its association with clinical and pathological characteristics of PTC. Subjects and methods: We retrospectively enrolled in the study a total of 43 consecutive PTC patients who underwent total thyroidectomy. We performed DNA extraction from formalin-fixed paraffin-embedded (FFPE) tumour tissue samples. Polymerase chain reaction (PCR) and direct sequencing were used to determine BRAF (V600E) mutation status. Univariate and multivariate logistic regression analyses were employed to identify independent associations. Results: The prevalence of BRAF (V600E) mutation was 65.1% (28/43). A high frequency of older patients (p value: 0.004) was observed among the BRAF-mutated PTC group and, in contrast, a low frequency of concurrent Hashimoto's thyroiditis (HT) (p value: 0.011) was noted. Multivariate analysis confirmed that older age (OR: 1.15; 95% CI: 1.00 – 1.33; p value: 0.047) and HT (OR: 0.05; 95% CI: 0.006-0.40; p value: 0.005) were independent factors associated with BRAF (V600E) mutation. Conclusion: We found a high prevalence of BRAF (V600E) mutation in PTC cases. Older age and no concurrent HT were independently associated with BRAF (V600E) mutation.
DOI: 10.6084/m9.figshare.7898996
title: Sources for the history of gynecology and obstetrics in Brazil

description: Abstract This article contributes to the debate about the medicalization of childbirth in Brazil through a literature review of databases of Brazilian libraries. Its main goal is to enable the systematized availability of specialized scientific publications in gynecology and obstetrics in the twentieth century, especially 1900 to 1950. In view of the importance of the analysis of journals in the development of scientific knowledge in Brazil and the importance of specialized journals in constituting and developing medical specializations, details of these archives are presented, including their location, when they were published, and where they were produced, paying particular attention to the archive in the library of the Teaching Maternity Hospital (Maternidade-Escola) of the Universidade Federal do Rio de Janeiro.
DOI: 10.6084/m9.figshare.7899002
title: POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells

description: During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1 overexpression, following the transfection of pCMVMycPod-1, significantly decreased the endogenous levels of Sf-1 mRNA and protein in F/R cells, but not in G cells, and also decreased the expression of the SF-1 target StAR in F/R cells. In G cells overexpressing POD-1, no modulation of the expression of SF-1 targets, StAR and CYP11B2, was observed. Our data showing that G and F/R cells respond differently to ectopic POD-1 expression emphasize the functional differences between the outer and inner zones of the adrenal cortex, and support the hypothesis that SF-1 is regulated by POD-1/Tcf21 in normal adrenocortical cells lacking the alterations in cellular physiology found in tumor cells.
DOI: 10.6084/m9.figshare.7899005.v1
title: In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae

description: The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The chemical compositions of the active extracts were investigated using high performance liquid chromatography. Eleven extracts obtained from five Annonaceae plant species were active and were particularly effective against the UA251, NCI-470 lung, HT-29, NCI/ADR, and K-562 cell lines with growth inhibition (GI50) values of 0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL, respectively. In addition, the Annona crassiflora and A. coriacea seed extracts were the most active among the tested extracts and the most effective against the tumor cell lines, with GI50 values below 8.90 µg/mL. The A. cacans extract displayed the lowest activity. Based on the microplate assay, the percent AchE inhibition of the extracts ranged from 12 to 52%, and the A. coriacea seed extract resulted in the greatest inhibition (52%). Caffeic acid, sinapic acid, and rutin were present at higher concentrations in the A. crassiflora seed samples. The A. coriacea seeds contained ferulic and sinapic acid. Overall, the results indicated that A. crassiflora and A. coriacea extracts have antiproliferative and anticholinesterase properties, which opens up new possibilities for alternative pharmacotherapy drugs.
DOI: 10.6084/m9.figshare.7898990
title: Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties

description: Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD50 &gt;1000 µg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC50 antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (P&lt;0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential.
DOI: 10.6084/m9.figshare.7898996.v1
title: Sources for the history of gynecology and obstetrics in Brazil

description: Abstract This article contributes to the debate about the medicalization of childbirth in Brazil through a literature review of databases of Brazilian libraries. Its main goal is to enable the systematized availability of specialized scientific publications in gynecology and obstetrics in the twentieth century, especially 1900 to 1950. In view of the importance of the analysis of journals in the development of scientific knowledge in Brazil and the importance of specialized journals in constituting and developing medical specializations, details of these archives are presented, including their location, when they were published, and where they were produced, paying particular attention to the archive in the library of the Teaching Maternity Hospital (Maternidade-Escola) of the Universidade Federal do Rio de Janeiro.
DOI: 10.6084/m9.figshare.7899002.v1
title: POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells

description: During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1 overexpression, following the transfection of pCMVMycPod-1, significantly decreased the endogenous levels of Sf-1 mRNA and protein in F/R cells, but not in G cells, and also decreased the expression of the SF-1 target StAR in F/R cells. In G cells overexpressing POD-1, no modulation of the expression of SF-1 targets, StAR and CYP11B2, was observed. Our data showing that G and F/R cells respond differently to ectopic POD-1 expression emphasize the functional differences between the outer and inner zones of the adrenal cortex, and support the hypothesis that SF-1 is regulated by POD-1/Tcf21 in normal adrenocortical cells lacking the alterations in cellular physiology found in tumor cells.
DOI: 10.6084/m9.figshare.7898981
title: Enhancement of anti-OVA IgG2c production in vivo by enalapril

description: Angiotensin-converting enzyme (ACE) inhibitors have non-hemodynamic, pleiotropic effects on the immune response. The effects of ACE inhibitors on the production of cytokines and T-cell functions are well established. However, little is known on the effects of these medicines on humoral response to foreign antigens. In this study, we investigated the effect of enalapril treatment on ovalbumin (OVA)-specific IgG1 and IgG2c production in mice determined by ELISA. Two groups of 8-week-old C57BL/6 females mice (3–4/group) were subcutaneously immunized with OVA (10 μg/animal) in presence of Alhydrogel (1 mg/mouse) and boosted at day 21. The mice were treated with enalapril (5 mg/kg daily, po) or were left without treatment for one month. The animals were bled from the orbital plexus on days 0, 7, 14, 21, and 28 after the first immunization and the sera were stored at –20°C until usage. OVA-specific serum IgG1 and IgG2c were determined by ELISA using serum from each individual animal. The results showed that enalapril significantly increased anti-OVA serum IgG2c in the secondary response without affecting IgG1 synthesis. These data expand our understanding on the properties of enalapril on the immune response, including antibody production.
DOI: 10.6084/m9.figshare.7898984
title: Reading of Students with Developmental Dyslexia: Impacts of an Intervention with Phonic Method Associated with the Executive Functions Stimuli

description: ABSTRACT: The aim of this study was to verify the impact of an intervention program involving the phonic method associated with executive functions (EF) in the performance of seven students with dyslexia with the mean age of 10.7 years (experimental group - EG), in tasks of executive functions (EF), phonemic awareness and reading. The performance of the EG was compared to three control groups: dyslexics (DCG) of the same age range of the EG; by age group (ACG) composed of seven boys of the same age group as the EG, and another group of skilled but younger readers (RCG) with four participants. The EG received the intervention in 28 sessions. The four groups were evaluated in phonemic awareness, reading, comprehension and the EF before and after the intervention received by the EG. As a result, the EG presented significantly higher performance than DCG in reading of isolated words (frequent, non-frequent and pseudowords). In reading comprehension, the two groups of dyslexics progressed in their performance, but not significantly. The intervention did not affect the performance of the EF of cognitive flexibility and working memory. The EG improved in orthographic verbal fluency and inhibitory control as measured by Go/No Go. The performance of the ACG and RCG did not change significantly in phonemic awareness, word reading, sentence and text comprehension, and most of the EF. These results allow to conclude that the participation of dyslexics in an intervention focusing on the development of phonemic awareness, reading and executive functions was efficient to promote their performance in reading, especially the reading of isolated words.
DOI: 10.6084/m9.figshare.7898987
title: Infant mortality in Santiago: representations and discourses, Chile, 1860-1914

description: Abstract The article explores the characteristics of infant mortality in Santiago during a historical period in which the demographic indicators reached very high figures turning the phenomenon into a medical and political issue. After commenting on historiographic data, it demonstrates the behavior of the infant population quantitatively, taking into account primary sources – from medicine, the press and civil records – and bibliography. It also exposes the motives employed by the elite to justify the children’s deaths: materially, morally and culturally. The conclusion addresses the promulgation of the defenseless child law, the beginnings of knowledge on child health and the inauguration of the Child Hospital.
DOI: 10.6084/m9.figshare.7898990.v1
title: Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties

description: Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD50 &gt;1000 µg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC50 antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (P&lt;0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential.
DOI: 10.6084/m9.figshare.7898975
title: Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro

description: Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP activity and calcium content were significantly enhanced by 10 μmol/L NaF with osteogenic inductive medium. Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. We conclude that moderate concentrations of NaF can stimulate proliferation and mineralization in periodontal ligament cells. These in vitro findings may provide a novel therapeutic approach for acceleration of periodontal regeneration by addition of suitable concentrations of NaF into the medication for periodontitis treatment, i.e., into periodontal packs and tissue patches.