DOI: 10.57760/sciencedb.12317
title: MicroRNA-223-3p stimulates proliferation, metastasis and regulates cell cycle via downregulating Neurofibromatosis Type 2 in Papillary thyroid cancer
title: MicroRNA-223-3p stimulates proliferation, metastasis and regulates cell cycle via downregulating Neurofibromatosis Type 2 in Papillary thyroid cancer

description: Papillary thyroid carcinoma (PTC) has become one of malignant diseases of endocrine system with the highest incidence rate especially among women. MicroRNA performed different roles in various cancer. MicroRNA-223-3p has been demonstrated to be highly transcribed in various cancerous cells to regulate tumor development. But the performance of microRNA-223-3p in PTC proliferation and metastasis is still unclear. Therefore, microRNA-223-3p and its predicted targeting protein NF-2 were examined in PTC tissues and cells by RT-qPCR and/or western blot assay. CCK-8 assay and transwell assay were carried out to evaluate the regulatory effect on cell proliferative and invasion respectively. Western blot was performed to evaluate N-cadherin, E-cadherin, Vimentin, MMP2 and MMP9 expression.Luciferase reporter assay has been achieved for examining the targeting sites in microRNA-223-3p with 3’UTR of NF-2. Moreover, the xenografts nude mice were utilized for accessing the roles of microRNA-223-3p on tumor increasing and KI-67 expression. According to our results, microRNA-223-3p was upregulated in PTC tissues and cells. The highly expression of microRNA-223-3p is related with poor prognosis in PTC patients. Moreover microRNA-223-3p mimics stimulates cell proliferative and invasive abilities in TPC-1 cells. And microRNA-223-3p could also induce the EMT of TPC-1 cells and up-regulate the expression of MMP2 and MMP9. Furthermore, luciferase reporter assays showed microRNA-223-3p can directly target NF-2 in TPC-1 cells. In addition, NF-2 overexpression could partially reverse the regulatory roles of microRNA-223-3p on PTC cell proliferation, invasion and EMT. The promotion impact of microRNA-223-3p on PTC cell was also confirmed through in vivo experiment. Therefore, microRNA-223-3p becomes a potential marker of PTC prognosis and microRNA-223-3p promotes the cell duplication and invasion through downregulating NF-2 in PTC cell lines. The discovery of microRNA-223-3p/NF-2 axis might pave a new way on the pathogenesis and therapy of PTC.
description: Papillary thyroid carcinoma (PTC) has become one of malignant diseases of endocrine system with the highest incidence rate especially among women. MicroRNA performed different roles in various cancer. MicroRNA-223-3p has been demonstrated to be highly transcribed in various cancerous cells to regulate tumor development. But the performance of microRNA-223-3p in PTC proliferation and metastasis is still unclear. Therefore, microRNA-223-3p and its predicted targeting protein NF-2 were examined in PTC tissues and cells by RT-qPCR and/or western blot assay. CCK-8 assay and transwell assay were carried out to evaluate the regulatory effect on cell proliferative and invasion respectively. Western blot was performed to evaluate N-cadherin, E-cadherin, Vimentin, MMP2 and MMP9 expression.Luciferase reporter assay has been achieved for examining the targeting sites in microRNA-223-3p with 3’UTR of NF-2. Moreover, the xenografts nude mice were utilized for accessing the roles of microRNA-223-3p on tumor increasing and KI-67 expression. According to our results, microRNA-223-3p was upregulated in PTC tissues and cells. The highly expression of microRNA-223-3p is related with poor prognosis in PTC patients. Moreover microRNA-223-3p mimics stimulates cell proliferative and invasive abilities in TPC-1 cells. And microRNA-223-3p could also induce the EMT of TPC-1 cells and up-regulate the expression of MMP2 and MMP9. Furthermore, luciferase reporter assays showed microRNA-223-3p can directly target NF-2 in TPC-1 cells. In addition, NF-2 overexpression could partially reverse the regulatory roles of microRNA-223-3p on PTC cell proliferation, invasion and EMT. The promotion impact of microRNA-223-3p on PTC cell was also confirmed through in vivo experiment. Therefore, microRNA-223-3p becomes a potential marker of PTC prognosis and microRNA-223-3p promotes the cell duplication and invasion through downregulating NF-2 in PTC cell lines. The discovery of microRNA-223-3p/NF-2 axis might pave a new way on the pathogenesis and therapy of PTC.