DOI: 10.5061/dryad.t4n42rr
title: Data from: Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation

description: Despite the ecological and societal importance of large rivers, fish
 sampling remains costly and limited to specific habitats (e.g., river
 banks). Using an eDNA metabarcoding approach, we regularly sampled 500 km
 of a large river (Rhône River). Comparisons with long-term electrofishing
 surveys demonstrated the ability of eDNA metabarcoding to qualitatively
 and quantitatively reveal fish assemblage structures (relative species
 abundance) but integrated a larger space than the classical sampling
 location. Combination of a literature review and field data showed that
 eDNA behaves in the water column like fine particulate organic matter. Its
 detection distance varied from a few km in a small stream to more than 100
 km in a large river. To our knowledge, our results are the first
 demonstration of the capacity of eDNA metabarcoding to describe
 longitudinal fish assemblage patterns in a large river, and metabarcoding
 appears to be a reliable, cost-effective method for future monitoring.
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