cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20455157
title: Evaluation of nefrotoxicity by tacrolimus and micophenolate mofetil associated with kidney ischemia and reperfusion: experimental study in rats

description: ABSTRACT Objective: to evaluate the renal toxicity caused by tacrolimus and mycophenolate mofetil (MMF) in a single kidney ischemia and reperfusion model. Method: experimental study using Wistar rats, submitted to right nephrectomy and left renal ischemia for 20 minutes, separated into groups in the postoperative period (PO): 1) Control (nonoperated); 2) Sham (operated, without PO drug); 3) TAC0.1, TAC1 and TAC10, tacrolimus administered PO at doses of 0.1mg/kg, 1mg/kg and 10mg/kg via gavage, respectively; 4) MMF, administered mycophenolate mofetil 20mg/kg; 5) MMF/TAC1 and MMF/TAC0.5, with an association of mycophenolate mofetil 20mg/kg and tacrolimus 1mg/kg and 0.5mg/kg, respectively. They were killed on the 14th PO and the kidney was removed for tissue oxidative stress analysis, by the dosage of reduced glutathione (GSH), lipoperoxidation (LPO) and protein carbonylation (PCO), and histological analysis by glomerular stereology (Glomerular volume density, Numerical density glomerular and mean glomerular volume). Renal function was evaluated by the measurement of serum creatinine and urea. Results: both drugs caused alterations in renal function, and the toxicity of tacrolimus was dose-dependent. Subacute toxicity did not show significant glomerular histological changes, and there was renal and compensatory glomerular hypertrophy in all groups except TAC10. Conclusion: Both drugs cause changes in renal function. Glomerular morphometry and stereology showed negative interference of immunosuppressants during compensatory glomerular hypertrophy.
DOI: 10.6084/m9.figshare.20455157.v1
title: Evaluation of nefrotoxicity by tacrolimus and micophenolate mofetil associated with kidney ischemia and reperfusion: experimental study in rats

description: ABSTRACT Objective: to evaluate the renal toxicity caused by tacrolimus and mycophenolate mofetil (MMF) in a single kidney ischemia and reperfusion model. Method: experimental study using Wistar rats, submitted to right nephrectomy and left renal ischemia for 20 minutes, separated into groups in the postoperative period (PO): 1) Control (nonoperated); 2) Sham (operated, without PO drug); 3) TAC0.1, TAC1 and TAC10, tacrolimus administered PO at doses of 0.1mg/kg, 1mg/kg and 10mg/kg via gavage, respectively; 4) MMF, administered mycophenolate mofetil 20mg/kg; 5) MMF/TAC1 and MMF/TAC0.5, with an association of mycophenolate mofetil 20mg/kg and tacrolimus 1mg/kg and 0.5mg/kg, respectively. They were killed on the 14th PO and the kidney was removed for tissue oxidative stress analysis, by the dosage of reduced glutathione (GSH), lipoperoxidation (LPO) and protein carbonylation (PCO), and histological analysis by glomerular stereology (Glomerular volume density, Numerical density glomerular and mean glomerular volume). Renal function was evaluated by the measurement of serum creatinine and urea. Results: both drugs caused alterations in renal function, and the toxicity of tacrolimus was dose-dependent. Subacute toxicity did not show significant glomerular histological changes, and there was renal and compensatory glomerular hypertrophy in all groups except TAC10. Conclusion: Both drugs cause changes in renal function. Glomerular morphometry and stereology showed negative interference of immunosuppressants during compensatory glomerular hypertrophy.
DOI: 10.6084/m9.figshare.20455159
title: Formulation and evaluation of the vascular endothelial growth factor loaded polycaprolactone nanoparticles

description: Abstract In an attempt to increase molecular stability and provide controlled release, vascular endothelial growth factor (VEGF) was encapsulated into polycaprolactone (PCL) nanoparticles. Both VEGF-free and VEGF-loaded PCL nanoparticles were formulated by w/o/w double emulsion of the dichloromethane-water system in the presence of polyvinyl alcohol (PVA) and rat serum albumin. To achieve the optimal formulation concerning particle size and monodispersity, studies were carried out with different formulation parameters, including PVA concentration, homogenization time and rate. Scanning electron microscopy and dynamic light scattering analysis showed respectively that particles had a spherical shape with a smooth surface and particle size varying between 58.68-751.9 nm. All of the formulations were negatively charged according to zeta potential analysis. In vitro release study was performed in pH 7.4 phosphate-buffered saline at 37°C and released VEGF amount was measured by enzyme-linked immunosorbent assay (ELISA) method. At the end of the 35th day, 10% of total encapsulated VEGF was released with a sustained-release profile, which fitted the Korsmeyer-Peppas kinetic model. The bioactivation of the nanoparticles was evaluated using XTT and ELISA methods. As a result, the released VEGF was biologically active and also VEGF loaded PCL nanoparticles enhanced proliferation of the human umbilical vein endothelial cells in cell culture.
DOI: 10.6084/m9.figshare.20455159.v1
title: Formulation and evaluation of the vascular endothelial growth factor loaded polycaprolactone nanoparticles

description: Abstract In an attempt to increase molecular stability and provide controlled release, vascular endothelial growth factor (VEGF) was encapsulated into polycaprolactone (PCL) nanoparticles. Both VEGF-free and VEGF-loaded PCL nanoparticles were formulated by w/o/w double emulsion of the dichloromethane-water system in the presence of polyvinyl alcohol (PVA) and rat serum albumin. To achieve the optimal formulation concerning particle size and monodispersity, studies were carried out with different formulation parameters, including PVA concentration, homogenization time and rate. Scanning electron microscopy and dynamic light scattering analysis showed respectively that particles had a spherical shape with a smooth surface and particle size varying between 58.68-751.9 nm. All of the formulations were negatively charged according to zeta potential analysis. In vitro release study was performed in pH 7.4 phosphate-buffered saline at 37°C and released VEGF amount was measured by enzyme-linked immunosorbent assay (ELISA) method. At the end of the 35th day, 10% of total encapsulated VEGF was released with a sustained-release profile, which fitted the Korsmeyer-Peppas kinetic model. The bioactivation of the nanoparticles was evaluated using XTT and ELISA methods. As a result, the released VEGF was biologically active and also VEGF loaded PCL nanoparticles enhanced proliferation of the human umbilical vein endothelial cells in cell culture.
DOI: 10.6084/m9.figshare.20455162
title: Perception of pharmacists regarding human resources, training, and development of a national hospital pharmacy: a preliminary report on Basel Statements

description: Abstract The hospital pharmacy needs a constant process of evaluation and monitoring of its activities. In Brazil, several agencies establish tasks and duties for pharmacists, but little is known about the compliance and the perception of the professional regarding these policies. The present study aims to characterize the pharmacist’s perception of Brazilian hospital pharmacy policies according to the Basel Statements. A search was performed for the contacts of all medium and high complexity hospitals in the country. Subsequently, a questionnaire elaborated following the Basel Statements was sent by e-mail to hospital pharmacists throughout the country. The domain analyzed was "Human Resources, Training, and Development". Statistical analyses were performed using SPSS 19.0. Pharmacists representing a total of 111 hospitals from all Brazilian regions answered the survey questionnaire and showed that more than half of the hospital pharmacists perceive themselves as not complying, whether in the service of local, national, or pharmaceutical education. Besides updating the professionals in relation to national policies, it is necessary that the representative bodies of the pharmaceutical class be more present in the elaboration and evaluation of the policies directed to human resources, seeking uniformity and the possibility of carrying out the activities required.
DOI: 10.6084/m9.figshare.20455162.v1
title: Perception of pharmacists regarding human resources, training, and development of a national hospital pharmacy: a preliminary report on Basel Statements

description: Abstract The hospital pharmacy needs a constant process of evaluation and monitoring of its activities. In Brazil, several agencies establish tasks and duties for pharmacists, but little is known about the compliance and the perception of the professional regarding these policies. The present study aims to characterize the pharmacist’s perception of Brazilian hospital pharmacy policies according to the Basel Statements. A search was performed for the contacts of all medium and high complexity hospitals in the country. Subsequently, a questionnaire elaborated following the Basel Statements was sent by e-mail to hospital pharmacists throughout the country. The domain analyzed was "Human Resources, Training, and Development". Statistical analyses were performed using SPSS 19.0. Pharmacists representing a total of 111 hospitals from all Brazilian regions answered the survey questionnaire and showed that more than half of the hospital pharmacists perceive themselves as not complying, whether in the service of local, national, or pharmaceutical education. Besides updating the professionals in relation to national policies, it is necessary that the representative bodies of the pharmaceutical class be more present in the elaboration and evaluation of the policies directed to human resources, seeking uniformity and the possibility of carrying out the activities required.
DOI: 10.6084/m9.figshare.20036539
title: Singular informal curriculum: electiveness in medical training during the pandemic

description: Abstract: Introduction: The interruption of teaching activities imposed by the Sars-CoV-2 pandemic offered a unique opportunity to analyze the extracurricular study habits of medical students, seeking to understand how the lack of a thematic institutional targeting impacted on medical training during the period of social isolation. Objective: This study aimed to evaluation of the occurrence, themes, and main sources of studies during the period of suspension of classes in the pandemic. Method: Analytical and descriptive study with data collected from a virtual questionnaire applied to medical students of a higher education institution. Result: From the complete suspension of local activities, of the 310 participants, 152 (49.0%) students claimed to have studied topics related to the health sciences and 93 (30.0%) studied topics not related to the health sciences area. Statistically, there was an exclusive preference for one area to another (p &lt; 0.001), indicating two study profiles in the period. The most abundant thematic interests were those addressed in virtual medical events, such as conferences or symposia, as well as those related to Covid-19. It stands out as the most cited themes: physiology, medical and general or specialized clinic, anatomy, languages, finance and philosophy. Conclusion: Despite the suspension of academic activities, students sought new learning habits according to their intellectual demands, denoting the development of a singularized informal curriculum.
DOI: 10.6084/m9.figshare.20036539.v1
title: Singular informal curriculum: electiveness in medical training during the pandemic

description: Abstract: Introduction: The interruption of teaching activities imposed by the Sars-CoV-2 pandemic offered a unique opportunity to analyze the extracurricular study habits of medical students, seeking to understand how the lack of a thematic institutional targeting impacted on medical training during the period of social isolation. Objective: This study aimed to evaluation of the occurrence, themes, and main sources of studies during the period of suspension of classes in the pandemic. Method: Analytical and descriptive study with data collected from a virtual questionnaire applied to medical students of a higher education institution. Result: From the complete suspension of local activities, of the 310 participants, 152 (49.0%) students claimed to have studied topics related to the health sciences and 93 (30.0%) studied topics not related to the health sciences area. Statistically, there was an exclusive preference for one area to another (p &lt; 0.001), indicating two study profiles in the period. The most abundant thematic interests were those addressed in virtual medical events, such as conferences or symposia, as well as those related to Covid-19. It stands out as the most cited themes: physiology, medical and general or specialized clinic, anatomy, languages, finance and philosophy. Conclusion: Despite the suspension of academic activities, students sought new learning habits according to their intellectual demands, denoting the development of a singularized informal curriculum.
DOI: 10.6084/m9.figshare.20455165
title: USE OF ULTRA COARSE DROPLETS AT DIFFERENT SPRAY VOLUMES TO CONTROL WEEDS WITH DICAMBA AND GLYPHOSATE HERBICIDES

description: ABSTRACT The use of ultra-coarse droplets for herbicide application reduces the risk of drift but may compromise the coverage and efficacy of the treatment. The objective of this work was to evaluate the use of ultra-coarse droplets, in different spray volumes, in the application of dicamba herbicide in association with glyphosate, for the control of weeds in two field trials. The first trial was conducted in a 5 x 2 factorial design, with five spray volumes (58, 72, 96, 144, and 177 L ha-1) and two air induction flat-fan spray tips (MUG 110015 - MagnoJet and TTI 110015 - Teejet). The control efficiency of Commelina benghalensis and the spray deposition on the target were evaluated by detecting a tracer using spectrophotometry. The second was conducted in a 2 x 2 factorial scheme, using two volumes (100 and 125 L ha-1) and two tips (MUG 11002 and TTI 11002). The control efficiency of Conyza spp. and fresh matter were evaluated. The different spray volumes and tips did not differ in relation to the deposition of the tracer. The use of ultra-coarse droplets was adequate for weed control. The TTI and MUG tips gave similar and adequate control performance starting at 96 L ha-1. Increasing the volume above 100 L ha-1 did not result in better control.
DOI: 10.6084/m9.figshare.20455165.v1
title: USE OF ULTRA COARSE DROPLETS AT DIFFERENT SPRAY VOLUMES TO CONTROL WEEDS WITH DICAMBA AND GLYPHOSATE HERBICIDES

description: ABSTRACT The use of ultra-coarse droplets for herbicide application reduces the risk of drift but may compromise the coverage and efficacy of the treatment. The objective of this work was to evaluate the use of ultra-coarse droplets, in different spray volumes, in the application of dicamba herbicide in association with glyphosate, for the control of weeds in two field trials. The first trial was conducted in a 5 x 2 factorial design, with five spray volumes (58, 72, 96, 144, and 177 L ha-1) and two air induction flat-fan spray tips (MUG 110015 - MagnoJet and TTI 110015 - Teejet). The control efficiency of Commelina benghalensis and the spray deposition on the target were evaluated by detecting a tracer using spectrophotometry. The second was conducted in a 2 x 2 factorial scheme, using two volumes (100 and 125 L ha-1) and two tips (MUG 11002 and TTI 11002). The control efficiency of Conyza spp. and fresh matter were evaluated. The different spray volumes and tips did not differ in relation to the deposition of the tracer. The use of ultra-coarse droplets was adequate for weed control. The TTI and MUG tips gave similar and adequate control performance starting at 96 L ha-1. Increasing the volume above 100 L ha-1 did not result in better control.
DOI: 10.6084/m9.figshare.20455168
title: Categories of the International Classification of Functioning, Disability and Health in studies of people with aphasia: a scoping review

description: ABSTRACT Purpose: to identify, through a scoping review, the categories of the International Classification of Functioning, Disability and Health described in studies on aphasia. Methods: the search was performed by DECs, MeSH terms and free terms related to the “International Classification of Functioning, Disability and Health” and “aphasia” in five databases, EMBASE, LILACS, PubMed, Scopus and Web of Science, and four search engines of grey literature. The PRISMA recommendations were used. EndNote and Rayyan managers were used to remove duplicates and read titles, abstracts and full studies. Papers that contained “aphasia” and some aspect from the International Classification of Functioning, Disability and Health were eligible, published from 2001. The data recorded were: type of study, level of evidence, sample, objectives and categories. Literature Review: 1,366 studies were located in the databases and 341 in the grey literature. The mostly described first level categories in the 13 selected studies were: Structures of the Nervous System (Brain); Mental Functions (Language); Communication (Conversation); Support and Relationships (Immediate Family). Gender and age were some of the most identified Personal Factors. Conclusion: this review provides support to the use of the International Classification of Functioning, Disability and Health for performance with people with aphasia.
DOI: 10.6084/m9.figshare.20455168.v1
title: Categories of the International Classification of Functioning, Disability and Health in studies of people with aphasia: a scoping review

description: ABSTRACT Purpose: to identify, through a scoping review, the categories of the International Classification of Functioning, Disability and Health described in studies on aphasia. Methods: the search was performed by DECs, MeSH terms and free terms related to the “International Classification of Functioning, Disability and Health” and “aphasia” in five databases, EMBASE, LILACS, PubMed, Scopus and Web of Science, and four search engines of grey literature. The PRISMA recommendations were used. EndNote and Rayyan managers were used to remove duplicates and read titles, abstracts and full studies. Papers that contained “aphasia” and some aspect from the International Classification of Functioning, Disability and Health were eligible, published from 2001. The data recorded were: type of study, level of evidence, sample, objectives and categories. Literature Review: 1,366 studies were located in the databases and 341 in the grey literature. The mostly described first level categories in the 13 selected studies were: Structures of the Nervous System (Brain); Mental Functions (Language); Communication (Conversation); Support and Relationships (Immediate Family). Gender and age were some of the most identified Personal Factors. Conclusion: this review provides support to the use of the International Classification of Functioning, Disability and Health for performance with people with aphasia.
DOI: 10.6084/m9.figshare.20455171
title: Gas exchange and yield of grafted yellow passion fruit under salt stress and plastic mulching

description: ABSTRACT Irrigation with saline water is one of the main factors that limit gas exchange and yield of yellow passion fruit in the semi-arid region of Brazil. The use of wild species of Passiflora ssp. tolerant to salinity as a rootstock and the application of mulching with plastic film can attenuate the effects of salt stress. The objective of present study was to evaluate the application of plastic film mulching and irrigation with saline water on the gas exchange and yield of yellow passion fruit grafted on P. cincinnata. The experiment was carried out under field conditions, from September 2019 to February 2021, in a 2 × (2 × 2) factorial scheme, in randomized blocks, in split plots and four replications. The treatments were related to irrigation with low-salinity (0.5 dS m-1) and high-salinity (4.5 dS m-1) water in yellow passion fruit plants, accession ‘Guinezinho’, propagated by seed and grafted on Passiflora cincinnata in the soil without and with plastic mulching. Irrigation with 4.5 dS m-1 water reduced gas exchange and yield of yellow passion fruit, but did not affect plants in the plastic mulched soil. Yellow passion fruit grafted on P. cincinnata showed increases in CO2 assimilation rate, but it was not reflected in fruit yield. For cultivation under high salinity conditions (4.5 dS m-1), it is recommended to use yellow passion fruit propagated by seeds in the soil with plastic film mulching.
DOI: 10.6084/m9.figshare.20455171.v1
title: Gas exchange and yield of grafted yellow passion fruit under salt stress and plastic mulching

description: ABSTRACT Irrigation with saline water is one of the main factors that limit gas exchange and yield of yellow passion fruit in the semi-arid region of Brazil. The use of wild species of Passiflora ssp. tolerant to salinity as a rootstock and the application of mulching with plastic film can attenuate the effects of salt stress. The objective of present study was to evaluate the application of plastic film mulching and irrigation with saline water on the gas exchange and yield of yellow passion fruit grafted on P. cincinnata. The experiment was carried out under field conditions, from September 2019 to February 2021, in a 2 × (2 × 2) factorial scheme, in randomized blocks, in split plots and four replications. The treatments were related to irrigation with low-salinity (0.5 dS m-1) and high-salinity (4.5 dS m-1) water in yellow passion fruit plants, accession ‘Guinezinho’, propagated by seed and grafted on Passiflora cincinnata in the soil without and with plastic mulching. Irrigation with 4.5 dS m-1 water reduced gas exchange and yield of yellow passion fruit, but did not affect plants in the plastic mulched soil. Yellow passion fruit grafted on P. cincinnata showed increases in CO2 assimilation rate, but it was not reflected in fruit yield. For cultivation under high salinity conditions (4.5 dS m-1), it is recommended to use yellow passion fruit propagated by seeds in the soil with plastic film mulching.
DOI: 10.6084/m9.figshare.20455174
title: Clinical application of the International Classification of Functioning, Disability and Health (ICF) in children and adolescents from a public hearing rehabilitation service

description: ABSTRACT Purpose: to develop an International Classification of Functioning, Disability and Health (ICF) Checklist for clinical application in the rehabilitation of children and adolescents presented with hearing loss, to describe the biopsychosocial profile of participants and present the results of the use of ICF by speech therapists. Methods: a descriptive cross-sectional observational research, with quantitative and qualitative analyses. The study was conducted with five speech therapists, eight children aged 7 to 10 years and eight adolescents between 11 and 18 years old, diagnosed with moderate to profound bilateral hearing loss. Data collection included interviews, analysis of medical records and a questionnaire for the speech therapists. Exploratory data analysis was performed using summary measures and descriptive analysis of the profile of participants. Results: an ICF Checklist for clinical use in Hearing Rehabilitation was created. With its application, deficiencies related to hearing, speech and language functions were observed, with considerable difficulties in expressive and receptive language skills, learning and communication and access barriers related to communication and health services. Conclusion: the use of the Checklist expands the aspects observed by professionals, allows identification of the subjects' needs, and enables the recording and monitoring of worsening, stabilization or progress of the biopsychosocial aspects in the rehabilitation process.