DOI: 10.22002/pzet2-gym95
title: Caulobacter crescentus

description: wild type CB15N. 1 of 7, no warping during imaging, good contrast. small, almost straight swarmer; no S-layer; 3 light & 2 dark storage granules; possible filaments at low end; central amorphous density 2-3rds cell length; one pilus; >4 bowl-like, dark (membrane?) disks
description: <strong>Tilt Series Date:</strong> 2006-09-26</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ddi-water ATCC (PYE) media;  cultured in 50ml flask; 7 days growth + passage + 5 days growth; see DPD notebook Sep 20 & 25, 2006</p>
<strong>Sample Preparation:</strong> box 697, grid 2; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 0.5 sec; see DPD notebook Sep 25, 2006</p>
  SAVE_06Sep26t01_xy.jpg: file associated to 3D Image #344 SAVE_bowl_01_xz.jpg: file associated to 3D Image #344 SAVE_fil_01_yz40.jpg: file associated to 3D Image #344 SAVE_fil_02_zx33.jpg: file associated to 3D Image #344 SAVE_patch_yz.jpg: file associated to 3D Image #344 SAVE_patch_zx30.jpg: file associated to 3D Image #344 SAVE_ribofil_01_xy.jpg: file associated to 3D Image #344 SAVE_ribofil_01_yz.jpg: file associated to 3D Image #344 SAVE_ribofil_01b_yz.jpg: file associated to 3D Image #344 SAVE_ribofil_02_xy.jpg: file associated to 3D Image #344 SAVE_ribofil_02_yz.jpg: file associated to 3D Image #344 SAVE_ribofil_03_xy.jpg: file associated to 3D Image #344 SAVE_ribofil_03_yz30.jpg: file associated to 3D Image #344 pdd2006-09-26-1_slicer10048.jpg: nested vesicle, Caulobacter crescentus pdd2006-09-26-1_slicer10053.jpg: flat vesicle, Caulobacter crescentus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-26-1/rawdata/</p>CC06Sep26t01.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-26-1/rawdata/CC06Sep26t01.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC06Sep26t01_18kx.mrc,  Tilt Series (Pixel Size 1.2 nm), 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-26-1/rawdata/CC06Sep26t01_18kx.mrc"
        > <i class="download icon"></i> Download </a></p>

        06Sep26t01bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-26-1/3dimage_344/06Sep26t01bn_full.rec"
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        SAVE_06Sep26t01_xy.tif,  file associated to 3D Image #344, 4.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-26-1/file_780/SAVE_06Sep26t01_xy.tif"
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        SAVE_bowl_01_xz.tif,  file associated to 3D Image #344, 5.1 MB  
        
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        SAVE_fil_01_yz40.tif,  file associated to 3D Image #344, 5.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-26-1/file_784/SAVE_fil_01_yz40.tif"
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        SAVE_fil_02_zx33.tif,  file associated to 3D Image #344, 6.6 MB  
        
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        SAVE_patch_yz.tif,  file associated to 3D Image #344, 5.8 MB  
        
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        SAVE_patch_zx30.tif,  file associated to 3D Image #344, 5.8 MB  
        
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        SAVE_ribofil_01_xy.tif,  file associated to 3D Image #344, 5.8 MB  
        
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        SAVE_ribofil_01_yz.tif,  file associated to 3D Image #344, 5.8 MB  
        
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        SAVE_ribofil_01b_yz.tif,  file associated to 3D Image #344, 5.8 MB  
        
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        SAVE_ribofil_02_xy.tif,  file associated to 3D Image #344, 5.8 MB  
        
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        SAVE_ribofil_02_yz.tif,  file associated to 3D Image #344, 5.9 MB  
        
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        SAVE_ribofil_03_xy.tif,  file associated to 3D Image #344, 6.1 MB  
        
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        SAVE_ribofil_03_yz30.tif,  file associated to 3D Image #344, 5.5 MB  
        
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