DOI: 10.5061/dryad.277g0
title: Data from: Evasion of predators contributes to the maintenance of male eyes in sexually dimorphic Euphilomedes ostracods (Crustacea)

description: Sexual dimorphisms have long drawn the attention of evolutionary
 biologists. However, we still have much to learn about the evolutionary,
 genetic, and developmental drivers of sexual dimorphisms. Here, we
 introduce ostracods of the genus Euphilomedes (Myodocopida, Ostracoda, and
 Crustacea) as a promising new system in which to investigate why and how
 sexual dimorphisms evolve. First, we ask whether male-skewed selective
 pressure from pelagic predators may help explain a dramatic sexual
 dimorphism in which male Euphilomedes have compound eyes, but females do
 not. Manipulative experiments demonstrate that blindfolding reduces the
 survival rate of male Euphilomedes when they are exposed to predatory
 fish. Blindfolding of the female rudimentary eyes (rudiments) does not,
 however, similarly influence the survival rate of brooding females.
 Further, numerical estimates of sighting distances, based on reasonable
 extrapolations from Euphilomedes’s eye morphology, suggest that the eyes
 of male Euphilomedes are useful for detecting objects roughly the size of
 certain pelagic predators, but not conspecifics. We conclude that eyes do
 not mediate direct interactions between male and female Euphilomedes, but
 that differences in predation pressure—perhaps associated with different
 reproductive behaviors—contribute to maintaining the sexually dimorphic
 eyes of these ostracods. Second, through transcriptome sequencing, we
 examined potential gene regulatory networks that could underlie sexual
 dimorphism in Euphilomedes’ eyes. From the transcriptome of juvenile male
 Euphilomedes’ eyes, we identified phototransduction genes and components
 of eye-related developmental networks that are well characterized in
 Drosophila and other species. The presence of suites of eye regulatory
 genes in our Euphilomedes juvenile male transcriptome will allow us, in
 future studies, to test how ostracods regulate the development of their
 sexually dimorphic eyes.
description: Assembled fasta contigs
 from E. carcharodonta juvenile male eye sequencingWe extracted RNA from
 dissected male eyes, made cDNA and sequenced with Illumina Ultra High
 Throughput Sequencing. Assembled with Trinity and
 iAssembler.EcJME_contigs.fastaEcJME_protein_ORFsEcJME_R1-part1This is part 1 of the forward raw reads from Illumina sequencing. Please download all three parts and recombine them using the following command in Terminal or Command Prompt: cat xaa xab xac &gt; EcJME_R1.fastqsangerxaaEcJME_R1-part2This is part 2 of the forward raw reads from Illumina sequencing. Please download all three parts and recombine them using the following command in Terminal or Command Prompt: cat xaa xab xac &gt; EcJME_R1.fastqsangerxabEcJME_R1-part3This is part 3 of the forward raw reads from Illumina sequencing. Please download all three parts and recombine them using the following command in Terminal or Command Prompt: cat xaa xab xac &gt; EcJME_R1.fastqsangerxacEcJME_R2-part1This is part 1 of the reverse raw reads from Illumina sequencing. Please download all three parts and recombine them using the following command in Terminal or Command Prompt: cat xaa2 xab2 xac2 &gt; EcJME_R2.fastqsangerxaa2EcJME_R2-part2This is part 2 of the reverse raw reads from Illumina sequencing. Please download all three parts and recombine them using the following command in Terminal or Command Prompt: cat xaa2 xab2 xac2 &gt; EcJME_R2.fastqsangerxab2EcJME_R1-part3This is part 3 of the reverse raw reads from Illumina sequencing. Please download all three parts and recombine them using the following command in Terminal or Command Prompt: cat xaa2 xab2 xac2 &gt; EcJME_R2.fastqsangerxac2eyegenehitsAnnotated predicted peptides of all E. carcharodonta sequences used in our phylogenetic analyses.