DOI: 10.5061/dryad.660js04
title: Data from: B cell receptor and Toll-like receptor signaling coordinate to control distinct B-1 responses to both self and the microbiota

description: B-1a cells play an important role in mediating tissue homeostasis and
 protection against infections. They are the main producers of
 spontaneously secreted serum antibodies predominately reactive to self
 antigens, like phosphatidylcholine (PtC), or to the intestinal microbiota.
 The mechanisms that regulate the B-1a immunoglobulin (Ig) repertoire and
 their antibody secretion remain poorly understood. Here, we use a novel
 reporter mouse to demonstrate that production of self- and
 microbiota-reactive antibodies is linked to BCR signaling in B-1a cells.
 Moreover, we show that Toll-like receptors (TLRs) are critical for shaping
 the Ig repertoire of B-1a cells as well as regulating their antibody
 production. Strikingly, we find that both the colonization of a microbiota
 as well as microbial-sensing TLRs are required for anti-microbiota B-1a
 responses, whereas nucleic-acid sensing TLRs are required for anti-PtC
 responses, demonstrating that linked activation of BCR and TLRs controls
 steady state B-1a responses to both self and microbiota-derived antigens.
description: Spleen_B-1a_TLRKO_2Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87686_pep.csvPerC_B-1a_TLRKO_3Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#3) 7wk old Peritoneal Cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87688_pep.csvPerC_B-1a_TLRKO_1Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#1) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87692_pep.csvSpleen_B-1a_TLRKO_3Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#3) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87695_pep.csvSpleen_B-1a_TLRKO_1Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#1) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87697_pep.csvPerC_B-1a_TLRKO_2Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#2) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87701_pep.csvPerC_B-1a_TLR2x4dKO_2Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#2) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.89642_pep.csvSpleen_B-1a_TLR2x4dKO_1Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#1) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.89641_pep.csvSpleen_B-1a_TLR2x4dKO_3Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#3) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.89640_pep.csvPerC_B-1a_TLR2x4dKO_1Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#1) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.89639_pep.csvPerC_B-1a_TLR2x4dKO_3Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#3) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.89638_pep.csvSpleen_B-1a_TLR2x4dKO_2Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.89637_pep.csvPerC_B-1a_Unc3d_2Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#2) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87705_pep.csvSpleen_B-1a_Unc3d_2Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87704_pep.csvSpleen_B-1a_Unc3d_3Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#3) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87703_pep.csvPerC_B-1a_Unc3d_3Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#3) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87702_pep.csvSpleen_B-1a_Unc3d_1Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#1) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87700_pep.csvPerC_B-1a_Unc3d_1Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#1) 7wk old Peritoneal Cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87696_pep.csvPerC_B-1a_WT_3WT C57BL/6 (Mus musculus) Female (#3) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87698_pep.csvPerC_B-1a_WT_1WT C57BL/6 (Mus musculus) Female (#1) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87694_pep.csvPerC_B-1a_WT_2WT C57BL/6 (Mus musculus) Female (#2) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc.87693_pep.csv87691_pepWT C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.Spleen_B-1a_WT_1WT C57BL/6 (Mus musculus) Female (#1) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87689_pep.csvSpleen_B-1a_WT_3WT C57BL/6 (Mus musculus) Female (#3) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc.87699_pep.csv