cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/etk8d-yrf82
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-27/rawdata/</p>20131221_YWC063_10027.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-27/rawdata/20131221_YWC063_10027.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_10027_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-27/3dimage_36392/20131221_YWC063_10027_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4hckp-x7z16
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-07-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-15/3dimage_11457/</p>RCC745_MGtovis_113.rec,  Reconstruction (Pixel Size 1.26 nm), 383.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-15/3dimage_11457/RCC745_MGtovis_113.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_113.mrc,  Tilt Series (Pixel Size 1.26 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-15/rawdata/RCC745_MGtovis_113.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wjtp8-jwe91
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-04-25</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-04-25-48/3dimage_11340/</p>RCC745_MGtovis_051.rec,  Reconstruction (Pixel Size 1.26 nm), 586.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-04-25-48/3dimage_11340/RCC745_MGtovis_051.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_051.mrc,  Tilt Series (Pixel Size 1.26 nm), 654.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-04-25-48/rawdata/RCC745_MGtovis_051.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/keqd9-dnt88
title: Myxococcus xanthus JK328

description: Bactofilin BacMNOP knockout strain. Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-07-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-07-22-68_slicer9113.jpg: short tube, Myxococcus Xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-68/rawdata/</p>07222013_Janelia_Myxo_JK328_20010.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-68/rawdata/07222013_Janelia_Myxo_JK328_20010.mrc"
        > <i class="download icon"></i> Download </a></p>

        07222013_Janelia_Myxo_JK328_20010.rec,  Reconstruction (Pixel Size 1.68 nm), 861.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-68/3dimage_28560/07222013_Janelia_Myxo_JK328_20010.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pj361-4nt47
title: Skinny E. coli DS664

description: Raw data files of Skinny E. coli DS664
description: <strong>Tilt Series Date:</strong> 2009-11-23</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> Cells provided by Daisuke Shiomi (BW25113 yhdE_deletion mreB-A125V). Data colleted by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Exponential phase in L Broth</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1 or R2/2, 10 nm gold, 100% humidity</p>
  zla2009-11-23-59_slicer9813.jpg: Escherichia coli .  vesicle zla2009-11-23-59_slicer9814.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-59/rawdata/</p>09nov23a_DS664_059.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-59/rawdata/09nov23a_DS664_059.mrc"
        > <i class="download icon"></i> Download </a></p>

        09nov23a_DS664_059_part.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-59/3dimage_9743/09nov23a_DS664_059_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/crd5f-xrq90
title: magnetosome induced t1

description: Raw data files of magnetosome induced t1
description: <strong>Tilt Series Date:</strong> 2014-06-19</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum </p>
<strong>Strain:</strong> deltaQ 167</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
  psa2014-06-19-12_slicer6698.jpg: dividing psa2014-06-19-12_slicer6699.jpg: tube psa2014-06-19-12_slicer6700.jpg: budding vesicle psa2014-06-19-12_slicer6701.jpg: peptidoglycan psa2014-06-19-12_slicer6702.jpg: filament psa2014-06-19-12_slicer6703.jpg: chemoreceptor psa2014-06-19-12_slicer6704.jpg: filament psa2014-06-19-12_slicer8046.jpg: magnetosome vesicle or filamnet or both psa2014-06-19-12_slicer8047.jpg: magnetosome vesicle or filamnet or both psa2014-06-19-12_slicer8048.jpg: magnetosome vesicle or filamnet or both
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-06-19-12/rawdata/</p>atlas40008.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-06-19-12/rawdata/atlas40008.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas40008_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-06-19-12/3dimage_43765/atlas40008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fwfqz-me213
title: Myxococcus xanthus delta-tsaP

description: grid box YWC065
description: <strong>Tilt Series Date:</strong> 2013-12-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-59/rawdata/</p>20131227_YWC065_10060.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-59/rawdata/20131227_YWC065_10060.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131227_YWC065_10060_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-59/3dimage_36783/20131227_YWC065_10060_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xkbzd-zzn53
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-15/rawdata/</p>20140308_YWC093_10008.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-15/rawdata/20140308_YWC093_10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140308_YWC093_10008_part131_5.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-15/3dimage_40027/20140308_YWC093_10008_part131_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/76bt9-hy005
title: O. tauri G1

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-12-10</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-12-10-44/rawdata/</p>RCC745_G1tovis_374.mrc,  Tilt Series (Pixel Size 0.96 nm), 671.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-12-10-44/rawdata/RCC745_G1tovis_374.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_G1tovis_374.rec,  Reconstruction (Pixel Size 0.96 nm), 354.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-12-10-44/3dimage_12339/RCC745_G1tovis_374.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z9zqn-r7m08
title: Sulfolobus cells synchronized for division

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2010-12-04</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity</p>
  mdj2010-12-04-12_slicer8253.jpg: ring .  Sulfolobus acidocaldarius mdj2010-12-04-12_slicer8257.jpg: ring .  Sulfolobus acidocaldarius mdj2010-12-04-12_slicer8259.jpg: OMV .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2010-12-04-12/rawdata/</p>10dec04b_Box13grid3_012new.mrc,  Tilt Series (Pixel Size 1.258 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2010-12-04-12/rawdata/10dec04b_Box13grid3_012new.mrc"
        > <i class="download icon"></i> Download </a></p>

        10dec04b_Box13grid3_012new_part.rec,  Reconstruction (Pixel Size 2.38 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2010-12-04-12/3dimage_19134/10dec04b_Box13grid3_012new_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5waqv-a1662
title: AimB depletion control w/ xylose

description: Raw data files of AimB depletion control w/ xylose
description: <strong>Tilt Series Date:</strong> 2009-09-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> The AimB_depletion strain was constructed by John Werner, data collected by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity</p>
  zla2009-09-21-61_slicer10196.jpg: Caulobacter crescentus .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-61/rawdata/</p>09sep21a_AimBxyl_025.mrc,  Tilt Series (Pixel Size 1.192 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-61/rawdata/09sep21a_AimBxyl_025.mrc"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_025_full.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-61/3dimage_10432/09sep21a_AimBxyl_025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cyz6h-xgk86
title: Sulfolobus cells synchronized for division

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2010-12-04</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity</p>
  mdj2010-12-04-9_slicer8231.jpg: filament .  Sulfolobus acidocaldarius mdj2010-12-04-9_slicer8233.jpg: pili .  Sulfolobus acidocaldarius mdj2010-12-04-9_slicer8235.jpg: vesicle? .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2010-12-04-9/rawdata/</p>10dec04b_Box13grid3_009new.mrc,  Tilt Series (Pixel Size 1.258 nm), 1.1 GB  
        
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        10dec04b_Box13grid3_009new_full.rec,  Reconstruction (Pixel Size 2.38 nm), 1.0 GB  
        
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