DOI: 10.5061/dryad.r14bv
title: Data from: Reward-induced phasic dopamine release in the monkey ventral striatum and putamen

description: In-vivo voltammetry has successfully been used to detect dopamine release
 in rodent brains, but its application to monkeys has been limited. We have
 previously detected dopamine release in the caudate of behaving Japanese
 monkeys using diamond microelectrodes (Yoshimi 2011); however it is not
 known whether the release pattern is the same in various areas of the
 forebrain. Recent studies have suggested variations in the dopaminergic
 projections to forebrain areas. In the present study, we attempted
 simultaneous recording at two locations in the striatum, using fast-scan
 cyclic voltammetry (FSCV) on carbon fibers, which has been widely used in
 rodents. Responses to unpredicted food and liquid rewards were detected
 repeatedly. The response to the liquid reward after conditioned stimuli
 was enhanced after switching the prediction cue. These characteristics
 were generally similar between the ventral striatum and the putamen.
 Overall, the technical application of FSCV recording in multiple locations
 was successful in behaving primates, and further voltammetric recordings
 in multiple locations will expand our knowledge of dopamine reward
 responses.
description: Voltammetric recordings
 of behaving monkeysEach folder contain data
 files of one experimental day. Original COLOR.txt files. Each FSCV
 recording consists of 500 voltage points for 10ms (50kHz sampling).
 Recording was repeated at 10Hz, every 0.1s. In MFB stimulation, each trial
 was made for 20s (200 time points) and the electrical stimulation was
 delivered from 5s (from recording 50). Original COLOR data of MFB
 stimulation is a table of 200 x 500. In behavioral analysis, each recoring
 was made for 300s block (3000 time points), simultaneously for 4 channels.
 In most cases, Ch0:ventral striatum (acc, Cd), Ch1:putamen, Ch3:CS signal
 and Ch4:IR sensor monitoring the facial movement. To export full waveform
 data of multi-channel recording on TH-1, backgound subtraction was turned
 off. The original data, recorded as nA value, was exported as COLOR.txt
 files. Original COLOR data of behavioral task is a table of 3000 x 500. CS
 signal was simultaneously recorded on Ch2, and the CS timing during 300s
 block was exported as IT.txt files. The hight of CS signal indicate the
 type of trials, and the offset of CS is the timing of juice delivery. For
 free trials, similar timing signal is recorded and the juice was delivered
 at the offset of the signal, but no visual stimuli appeared on the screen.
 Original ITCS data of CS is a table of 3000 x 1. For food trials, the
 pulses in ITCS data indicate the timing of reaching of the food to the
 mouth. These data were loaded on MATLAB, background subtracted for each
 trials, and PCR was performed using princomp function. Templates for
 Principle Component Regression (PCR) analysis. Voltage-current curves (500
 points) at the peak MFB responses were collected as in-vivo dopamine
 template. Also, presumably pH curves were collected 10-15s after the
 stimulation. PCR results Result of PCR: 1zz, 2zz and 3zz files, each
 columns indicate one trial for 8s (80 points). Each column started with
 the Block number, timing point of CS onset in the block and hight of CS
 signal. The trials judged as "extreme" have more than 1000 value
 of CS signal. 1zz is the dopamine-like component and 2zz is the pH-like
 component. 3zz is the current at the peak of dopamine oxidation potential
 (120/500 points) Point 40 is the CS onset. Timing cue was shown from point
 30, and the onset of juice delivery was point 55. One behavioral session
 consists of multiple 5-min blocks. Review on EXEL show the average of each
 trial type within the session. In original analysis to calculate PCR
 result of 1zz, timing of the trials were aligned by CS onset, so time 0 is
 the time just before the CS onset. For final graph presentation of the
 results, the timing of juice delivery/CS offset is shown as time 0. In
 some review tables of EXEL, you need to find which timing determination is
 applied.YoshimiPlosTXTData-noJP.zip