DOI: 10.5061/dryad.6wwpzgmtv
title: Data from: Responses of activity rhythms to temperature cues evolve in Drosophila populations selected for divergent timing of eclosion

description: Even though the rhythm in adult emergence and rhythm in locomotor activity
 are two different rhythmic phenomena that occur at distinct life-stages of
 the fly life cycle, previous studies have hinted at similarities in
 certain aspects of the organisation of the circadian clock driving these
 two rhythms. For instance, the period gene plays an important regulatory
 role in both rhythms. In an earlier study, we have shown that selection on
 timing of adult emergence behaviour in populations of Drosophila
 melanogaster leads to the co-evolution of temperature sensitivity of
 circadian clocks driving eclosion. In this study, we were interested in
 asking if temperature sensitivity of the locomotor activity rhythm has
 evolved in our populations with divergent timing of adult emergence
 rhythm, with the goal of understanding the extent of similarity (or lack
 of it) in circadian organisation between the two rhythms. We found that in
 response to simulated jetlag with temperature cycles, late chronotypes
 (populations selected for predominant emergence during dusk) indeed
 re-entrain faster than early chronotypes (populations selected for
 predominant emergence during dawn) to 6-h phase-delays, thereby indicating
 enhanced sensitivity of the activity/rest clock to temperature cues in
 these stocks (entrainment is the synchronisation of internal rhythms to
 cyclic environmental time-cues). Additionally, we found that late
 chronotypes show higher plasticity of phases across regimes, day-to-day
 stability in phases and amplitude of entrainment, all indicative of
 enhanced temperature sensitive activity/rest rhythms. Our results
 highlight remarkably similar organisation principles between emergence and
 activity/rest rhythms.
description: Data were collected using the Drosophila Activity Monitor
 system (DAM, Trikinetics, Waltham, MA USA) under a wide variety of light
 and temperature regimes. Scanned monitor files have been uploaded here.
 Please contact either Lakshman Abhilash or Vasu Sheeba for more
 details.
description: <b>Description of data sets</b>
 Three sets of data files from three sets of experiments are
 deposited here.  The first set contains scanned DAM monitor files from 6-h
 phase advanced and 6-h phase delayed jetlag for all four replicates of
 <em>early</em>, <em>control</em> and
 <em>late</em> populations (total of 12 files per regime)
 (Figure 1).  The second set contains scanned DAM monitor files for all 12
 populations under and post entrainment to three temperature cycle regimes
 (three different thermoperiods) (Figures 2, 3 and 6).  The third set has
 scanned DAM monitor files for all 12 populations under and post
 entrainment to light/dark cycles under different ambient temperatures
 (Figures 4 and 5). <strong>Key for file
 names</strong> GE1 - <i>early</i>
 block-1 GE2 - <i>early</i>
 block-2 GE3 - <i>early</i>
 block-3 GE4 - <i>early</i>
 block-4 GC1 - <i>control</i>
 block-1 GC2 - <i>control</i>
 block-2 GC3 - <i>control</i>
 block-3 GC4 - <i>control</i>
 block-4 GL1 - <i>late</i> block-1
 GL2 - <i>late</i> block-2 GL3 -
 <i>late</i> block-3 GL4 -
 <i>late</i> block-4