cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/cwzzc-00k75
title: Rhesus Macaque Fibroblast

description: Proteasome inhibition Campbell et al 2008 Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate to any virus because light image only taken in the red channel. Discovered the autofluorescent problem when looking at fibroblasts with no virus, bright puncta everywhere and correlate to onions
description: <strong>Tilt Series Date:</strong> 2015-05-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr-Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift. Proteasome inhibition 200mg/ml</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-2/3dimage_57241/</p>tomography11.rec,  Reconstruction (Pixel Size 24.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-2/3dimage_57241/tomography11.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography11.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-2/rawdata/tomography11.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography11_full.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-2/3dimage_76014/tomography11_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jacfa-g0556
title: Rhesus Macaque Fibroblast

description: Proteasome inhibition Campbell et al 2008 Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate to any virus because light image only taken in the red channel. Discovered the autofluorescent problem when looking at fibroblasts with no virus, bright puncta everywhere and correlate to onions
description: <strong>Tilt Series Date:</strong> 2015-05-18</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr-Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift. Proteasome inhibition 200mg/ml</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-6/3dimage_57228/</p>tomography5_part121_19.rec,  Reconstruction (Pixel Size 24.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-6/3dimage_57228/tomography5_part121_19.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography5.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-6/rawdata/tomography5.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography5_full.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-6/3dimage_76010/tomography5_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.h257dc
title: Occurrence Download

description: A dataset containing 16621 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Maclura tinctoria"
 ]
}

 The dataset includes 16621 records from 431 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047310-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/41rca-vq322
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-64/3dimage_73321/</p>20150511_AK07_10064_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-64/3dimage_73321/20150511_AK07_10064_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10064.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-64/rawdata/20150511_AK07_10064.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10064_SIRT_2K.rec~,  5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-64/file_16622/20150511_AK07_10064_SIRT_2K.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.pxtyxr
title: Occurrence Download

description: A dataset containing 741 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Syrmaticus ellioti",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 741 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047312-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/es2j3-vnn18
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-72/3dimage_73327/</p>20150511_AK07_10072_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-72/3dimage_73327/20150511_AK07_10072_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10072.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-72/rawdata/20150511_AK07_10072.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10072_SIRT_2K.rec~,  5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-72/file_16628/20150511_AK07_10072_SIRT_2K.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kwq49-xr524
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-27/rawdata/</p>20160424_YWC212_20016.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-27/rawdata/20160424_YWC212_20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20016_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-27/3dimage_74809/20160424_YWC212_20016_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20016_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-27/3dimage_74810/20160424_YWC212_20016_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bva29-f7434
title: Saccharomyces cerevisiae - JAWY1

description: Raw data files of Saccharomyces cerevisiae - JAWY1
description: <strong>Tilt Series Date:</strong> 2016-10-31</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-10-31-4to5 (TS3,TS4)- Heat shocked @38 deg C for 2min10min. Other sm2016-10-31-* - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.</p>
<strong>Sample Preparation:</strong> Final OD of 0.2 on grids (in growth medium) and blotted from behind</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-2/3dimage_80813/</p>TS12comb_part121_1.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-2/3dimage_80813/TS12comb_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>

        TS12comb.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-2/rawdata/TS12comb.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS12comb_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 233.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-2/3dimage_81449/TS12comb_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cwexv-wnj90
title: Rhesus Macaque Fibroblast

description: Proteasome inhibition Campbell et al 2008 Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate to any virus because light image only taken in the red channel. Discovered the autofluorescent problem when looking at fibroblasts with no virus, bright puncta everywhere and correlate to onions
description: <strong>Tilt Series Date:</strong> 2015-05-18</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr-Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift. Proteasome inhibition 200mg/ml</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-10/3dimage_57236/</p>tomography9_part121_36.rec,  Reconstruction (Pixel Size 24.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-10/3dimage_57236/tomography9_part121_36.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography9.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-10/rawdata/tomography9.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography9_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-18-10/3dimage_76007/tomography9_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.29927774
title: (04) Band Structure Calculations (Lieb-like Lattices)

description: Band structure calculations for SCIGEN-generated materials with Lieb-like lattices.
DOI: 10.6084/m9.figshare.29927774.v2
title: (04) Band Structure Calculations (Lieb-like Lattices)

description: Band structure calculations for SCIGEN-generated materials with Lieb-like lattices.
DOI: 10.6084/m9.figshare.29927774.v1
title: (04) Band structure calculation of the materials with Lieb-like lattices from SCIGEN

description: The band structure calculation of Lieb-like materials generated by SCIGEN.
DOI: 10.22002/kt73p-mw286
title: Saccharomyces cerevisiae - JAWY1

description: Raw data files of Saccharomyces cerevisiae - JAWY1
description: <strong>Tilt Series Date:</strong> 2016-10-31</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-10-31-4to5 (TS3,TS4)- Heat shocked @38 deg C for 2min10min. Other sm2016-10-31-* - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.</p>
<strong>Sample Preparation:</strong> Final OD of 0.2 on grids (in growth medium) and blotted from behind</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-5/3dimage_80823/</p>TS4combined.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-5/3dimage_80823/TS4combined.rec"
        > <i class="download icon"></i> Download </a></p>

        TS4combined.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-5/rawdata/TS4combined.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS4combined_2bin_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 448.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-5/3dimage_81452/TS4combined_2bin_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v91ea-v3677
title: Sulfolobus cells synchronized for division

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2011-01-16</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 1.5 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on one sides, 6 sec, sponge for humidity</p>
  mdj2011-01-16-8_slicer8747.jpg: unknown .  Sulfolobus acidocaldarius mdj2011-01-16-8_slicer8748.jpg: filament .  Sulfolobus acidocaldarius mdj2011-01-16-8_slicer8749.jpg: filament .  Sulfolobus acidocaldarius mdj2011-01-16-8_slicer8751.jpg: pili .  Sulfolobus acidocaldarius mdj2011-01-16-8_slicer8885.jpg: ESCRT? .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-16-8/rawdata/</p>11jan16a_Bx13gr2_009.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-16-8/rawdata/11jan16a_Bx13gr2_009.mrc"
        > <i class="download icon"></i> Download </a></p>

        11jan16a_Bx13gr2_009_full.rec,  Reconstruction (Pixel Size 2.5 nm), 4.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-16-8/3dimage_19329/11jan16a_Bx13gr2_009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11jan16a_Bx13gr2_009_fullmedian.rec,  Reconstruction (Pixel Size 2.5 nm), 4.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-16-8/3dimage_19330/11jan16a_Bx13gr2_009_fullmedian.rec"
        > <i class="download icon"></i> Download </a></p>