DOI: 10.5061/dryad.05qfttf8z
title: Algal symbiont diversity in Acropora muricata from the extreme reef of Bouraké associated with resistance to coral bleaching

description: Widespread coral bleaching has generally been linked to high water
 temperatures at larger geographic scale. However, teh bleaching response
 can be highly variable aming individuals of the same species, between
 different species, and across localities; what causes this variability
 remais unresolved. Here, we tracked bleached and non-bleached colonies of
 Acropora muricata to see if they recovered or died following a
 stress event inside the semi-enclosed lagoon of Bouraké (New Caledonia),
 where corals are long-term acclimatized to extreme conditions of
 temperature, pH and dissolved oxygen, and at a nearby control reef. We
 describe Symbiodiniaceae community changes, metabolic responses, and
 energetical reserves (12 physiological traits evaluated) during La Niña
 warm and rainy summer in 2021. Widespread coral bleaching (score 1 and 2
 on coral colour health chart) was observed only in Bouraké, likely due to
 the combination of the high temperatures (up to 32 oC) and heavy rain. All
 colonies (i.e., Bouraké and reference site) had Cladocopium  as
 their main genera. Unbleached colonies in Bouraké had a specific ITS2-type
 profile (proxies for Symbiodiniaceae genotypes), while the bleached
 colonies in Bouraké had the same ITS2-type profile of control colonies
 during the stress event. After four months, teh few bleached colonies
 thata survived acquired the same ITS2 type profiles of the unbleached
 resistant colonies. In terms of physiological performances, all bleached
 corals showed metabolic depression (e.g., Pgross and Rdark). In contrast,
 unbleahed colonies in Bouraké maintained higher metabolic rates and
 energetic reserves compared to control corals. Our study suggests that
 Acropora muricata enhanced their resistance to bleaching thanks to
 specific Symbiodiniaceae, while energetic reserves may increase their
 resilience after stress. 
description: See the mauscript for the methods
description: # **Algal symbiont diversity in *Acropora muricata* from the extreme reef
 of Bouraké associated with resistance to coral bleaching**
 [https://doi.org/10.5061/dryad.05qfttf8z](https://doi.org/10.5061/dryad.05qfttf8z) There are four main files. The first file (physio) reports the physiological responses of bleached and unbleached corals inhabiting the extreme semi-lagoon of Bouraké (New Caledonia). ## Description of the data and file structure File name: Gross photosynthesis Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 61 rows x 6 columns \* Pg: gross photosynthesis \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: respiration Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 61 rows x 6 columns \* R: respiration \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Pg-R Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 60 rows x 6 columns \* R: respiration \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Lipid Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 57 rows x 6 columns \* lipids mg gdw: lipid mg gdw \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: protein Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 43 rows x 6 columns \* protein\_mgcm: protein mg cm-2 \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: carbohydrates Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 56 rows x 6 columns \* carbs\_mg gdw: carbohydrates mg gdw \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Biomass Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 57 rows x 6 columns \* Biomass: Biomass mg cm-2 \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Symbiont count Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 10 KB \* Dimensions: 61 rows x 6 columns \* zooxcm: number of zooxanthellae cm-2 \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Chlorophyll Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 10 KB \* Dimensions: 43 rows x 6 columns \* Chltotcm: Chlorophyll cm-2 \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: ETRmax Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 57 rows x 6 columns \* ETRmax: maximum electron transport rate \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Yield Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 57 rows x 6 columns \* Yield: photosynthetic efficiency \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: Growth rate Description: data are reported in an excel file containing the relative to all physiological parameters used in this study. Format(s): .xls \* Size(s): 11 KB \* Dimensions: 46 rows x 6 columns \* Growth rate: growth rate \* ID: the ID of the coral sample \* Condition: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Origin: the site of origin \* State: defines the health status \* Time: time of sampling File name: ITS2 results profiles Description: data are reported in an csv file containing the total sequences defining the ITS2 type profile that have been used to produce Figure 5. Format(s): .csv \* Size(s): 5 KB \* Dimensions: 61 rows x 21 columns \* From column 5 to 21 there are the main ITS2 type profile \* ID: the ID of the coral sample \* Treat: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Site: the site of origin \* Time: time of sampling File name: ITS2 results Description: data are reported in an csv file containing the relative sequences defining the ITS2 type of each coral individual. These data have been used to produce Figure 5. Format(s): .csv \* Size(s): 32 KB \* Dimensions: 61 rows x 168 columns \* Variables: ITS2 type profile \* ID: the ID of the coral sample \* Treat: the category that define coral health status and provenience (BZ = Bouraké healthy, BB = Bouraké bleached; RZ = Reference healthy) \* Site: the site of origin \* Time: time of sampling File name: Seawater temperature Description: Seawater data temperature recorded each 10 minutes from the 1st of December 2020 to the 31st of May 2021. Format(s): .xls \* Size(s): 4.9 MB \* Dimensions: 52328 rows x 4 columns \* Temp: Seawater temperature \* month: month of data recording \* day: day of the month \* Site: site investigated \* Date: full date File name: community\_4 categories Description: data on coral cover defined as 4 categories (Healthy, bleached, recently dead, old dead). Format(s): .csv \* Size(s): 22 KB \* Dimensions: 721 rows x 6 columns \* Percentage: percentage of coral cover \* State: the four category used in the article (i.e., healthy, bleached, recently dead, old dead) \* Transect: number of transect \* Quadrate: number of quadrate (50x50cm) \* Site: the site of origin \* Time: time of sampling ## ## Sharing/Access information This is a section for linking to other ways to access the data, and for linking to sources the data is derived from, if any. Links to other publicly accessible locations of the data: * [http://...](http://...) Data was derived from the following sources: * ## Code/Software This is an optional, freeform section for describing any code in your submission and the software used to run it. Describe any scripts, code, or notebooks (e.g., R, Python, Mathematica, MatLab) as well as the software versions (including loaded packages) that you used to run those files. 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