cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.13489778
title: Prediction Error of Intraocular Lens Power Calculation in Very Elderly Patients over 90 Years Old

description: To investigate the influence of age on prediction error (PE) after cataract surgery in very elderly (VE) patients aged more than 90 years. We retrospectively analyzed 66 eyes of patients aged ≥90 years (VE group) who underwent phacoemulsification and intraocular lens (IOL) implantation. As the control group (CG), we investigated 121 eyes of patients aged 70–89 years who underwent the same surgery. PE was calculated 1 month post-surgery as the actual postoperative spherical equivalent minus the target diopter, which was calculated using the Sanders–Retzlaff–Kraff/T formula. The absolute and arithmetic PE were compared between the two groups. The factors affecting absolute PE outside ±0.5 diopter (D) and ±1.0 D were determined through logistic regression analysis with the variables age, sex, axial length (AL), average corneal power, preoperative best-corrected visual acuity, target diopter, and coexisting pseudoexfoliation syndrome. The absolute PE was significantly larger in the VE group than that in the CG (0.60 ± 0.52 D and 0.34 ± 0.25 D, respectively; <i>P</i> &lt; .001). There was no significant difference in terms of arithmetic PE between the two groups (−0.06 ± 0.79 D and −0.07 ± 0.42 D, respectively; <i>P</i> = .653). In the logistic regression analysis, age was significantly associated with absolute PE outside ±0.50 D (Odds ratio [OR]: 1.05). Age and AL were significantly associated with absolute PE outside ±1.0 D (OR: 1.24 and 0.20, respectively). Absolute PE tended to increase in the cataract surgery of VE patients.
DOI: 10.6084/m9.figshare.13489778.v1
title: Prediction Error of Intraocular Lens Power Calculation in Very Elderly Patients over 90 Years Old

description: To investigate the influence of age on prediction error (PE) after cataract surgery in very elderly (VE) patients aged more than 90 years. We retrospectively analyzed 66 eyes of patients aged ≥90 years (VE group) who underwent phacoemulsification and intraocular lens (IOL) implantation. As the control group (CG), we investigated 121 eyes of patients aged 70–89 years who underwent the same surgery. PE was calculated 1 month post-surgery as the actual postoperative spherical equivalent minus the target diopter, which was calculated using the Sanders–Retzlaff–Kraff/T formula. The absolute and arithmetic PE were compared between the two groups. The factors affecting absolute PE outside ±0.5 diopter (D) and ±1.0 D were determined through logistic regression analysis with the variables age, sex, axial length (AL), average corneal power, preoperative best-corrected visual acuity, target diopter, and coexisting pseudoexfoliation syndrome. The absolute PE was significantly larger in the VE group than that in the CG (0.60 ± 0.52 D and 0.34 ± 0.25 D, respectively; <i>P</i> &lt; .001). There was no significant difference in terms of arithmetic PE between the two groups (−0.06 ± 0.79 D and −0.07 ± 0.42 D, respectively; <i>P</i> = .653). In the logistic regression analysis, age was significantly associated with absolute PE outside ±0.50 D (Odds ratio [OR]: 1.05). Age and AL were significantly associated with absolute PE outside ±1.0 D (OR: 1.24 and 0.20, respectively). Absolute PE tended to increase in the cataract surgery of VE patients.
DOI: 10.6084/m9.figshare.14198833
title: 3D Printable, Modified Trephine Designs for Consistent Anterior Lamellar Keratectomy Wounds in Rabbits

description: Our goal is to develop a low-cost tool that can be used to create consistent, partial-thickness defects in rabbit and other large animals with minimal surgical training and that can facilitate pre-clinical testing of lamellar and in situ-forming biosynthetic matrix materials for corneal repair. In this study, three modified trephines were designed to create deep corneal wound defects with consistent depth in large animals. The modified trephines incorporated either 3D-printed parts made from photopolymerizable resins, or custom-cut commercially available Teflon sheets. Wound defects were imaged with optical coherence tomography (OCT), and the depth was analyzed based on the OCT images. The results revealed that an inner-stopper guard trephine had the best performance in creating consistent and precise wound defect depth compared to modified vacuum trephine and custom guard vacuum trephine. A 75% ± 10% cut of the cornea was achieved with the inner-stopper guard trephine. The wound defect depth by created by the inner-stopper guard trephine was independent of the corneal thickness or size of the globes. Although the cut depth of the inner-stopper guard trephine differed by the experience-level of its users, the consistency (standard deviation) of the depth was independent of experience. Our studies provided three cost-efficient animal trephines that can create corneal wounds of consistent depth by lab researchers without extensive training in keratectomy.
DOI: 10.6084/m9.figshare.14198833.v1
title: 3D Printable, Modified Trephine Designs for Consistent Anterior Lamellar Keratectomy Wounds in Rabbits

description: Our goal is to develop a low-cost tool that can be used to create consistent, partial-thickness defects in rabbit and other large animals with minimal surgical training and that can facilitate pre-clinical testing of lamellar and in situ-forming biosynthetic matrix materials for corneal repair. In this study, three modified trephines were designed to create deep corneal wound defects with consistent depth in large animals. The modified trephines incorporated either 3D-printed parts made from photopolymerizable resins, or custom-cut commercially available Teflon sheets. Wound defects were imaged with optical coherence tomography (OCT), and the depth was analyzed based on the OCT images. The results revealed that an inner-stopper guard trephine had the best performance in creating consistent and precise wound defect depth compared to modified vacuum trephine and custom guard vacuum trephine. A 75% ± 10% cut of the cornea was achieved with the inner-stopper guard trephine. The wound defect depth by created by the inner-stopper guard trephine was independent of the corneal thickness or size of the globes. Although the cut depth of the inner-stopper guard trephine differed by the experience-level of its users, the consistency (standard deviation) of the depth was independent of experience. Our studies provided three cost-efficient animal trephines that can create corneal wounds of consistent depth by lab researchers without extensive training in keratectomy.
DOI: 10.6084/m9.figshare.13395668
title: LncRNA-MALAT1/miRNA-204-5p/Smad4 Axis Regulates Epithelial–Mesenchymal Transition, Proliferation and Migration of Lens Epithelial Cells

description: <b>Purpose</b>: Posterior capsular opacification (PCO), a common complication after cataract surgery, primarily originated from the epithelial–mesenchymal transition (EMT), proliferation, and migration of human lens epithelial cells (LECs). This study aimed to explore whether the long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/miR-204-5p/Smad4 axis are involved in EMT of LECs. LECs were cultured and induced with TGF-β2 (10 ng/mL). SiRNA against MALAT1 (Si‐MALAT1) was transfected into LECs to knockdown the expression of MALAT1. To overexpress or knockdown miR‐204-5p, miR-204-5p mimics (miR-204-5p mimics) and anti-miR-204-5p (miR-204-5p inhibitor) were transfected into LECs. We used RNA FISH to identify the location of MALAT1. RNA levels of MALAT1 and miR-204-5p were analyzed by RT-qPCR. Additionally, target protein levels of Smad4, epithelial differentiation and mesenchymal markers were analyzed with Western blot. We employed EdU Labeling to measured cell proliferation and performed Transwell Assay to analyze the cell migration. Dual-luciferase reporter assays in LECs were conducted to verify whether miRNA-204-5p was negatively regulated by MALAT1 and Smad4 was a direct target of miR-204-5p. The expression of MALAT1 was upregulated in PCO specimens. MALAT1 was overexpressed in TGF-β2 induced LECs, and the knockdown of MALAT1 could attenuate TGF-β2 induced EMT. Besides, the upregulation of MALAT1 was correlated with the downregulation of miR-204-5p and upregulation of Smad4. Importantly, MALAT1 was revealed to be located in the cytoplasm of LECs. Furthermore, luciferase reporter assays confirmed that MALAT1 could negatively regulate the expression of miR-204-5p and then regulate its direct target Smad4. Finally, the knockdown of MALAT1 could inhibit the EMT, proliferation, and migration of LECs; however, those can be reversed by anti-miR-204-5p. Our findings reveal that MALAT1 may regulate EMT, proliferation, and migration of LECs as a ceRNA by “sponging” miR-204-5p and targeting Smad4, and serve as a promising therapeutic target in preventing PCO.
DOI: 10.6084/m9.figshare.13395668.v1
title: LncRNA-MALAT1/miRNA-204-5p/Smad4 Axis Regulates Epithelial–Mesenchymal Transition, Proliferation and Migration of Lens Epithelial Cells

description: <b>Purpose</b>: Posterior capsular opacification (PCO), a common complication after cataract surgery, primarily originated from the epithelial–mesenchymal transition (EMT), proliferation, and migration of human lens epithelial cells (LECs). This study aimed to explore whether the long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/miR-204-5p/Smad4 axis are involved in EMT of LECs. LECs were cultured and induced with TGF-β2 (10 ng/mL). SiRNA against MALAT1 (Si‐MALAT1) was transfected into LECs to knockdown the expression of MALAT1. To overexpress or knockdown miR‐204-5p, miR-204-5p mimics (miR-204-5p mimics) and anti-miR-204-5p (miR-204-5p inhibitor) were transfected into LECs. We used RNA FISH to identify the location of MALAT1. RNA levels of MALAT1 and miR-204-5p were analyzed by RT-qPCR. Additionally, target protein levels of Smad4, epithelial differentiation and mesenchymal markers were analyzed with Western blot. We employed EdU Labeling to measured cell proliferation and performed Transwell Assay to analyze the cell migration. Dual-luciferase reporter assays in LECs were conducted to verify whether miRNA-204-5p was negatively regulated by MALAT1 and Smad4 was a direct target of miR-204-5p. The expression of MALAT1 was upregulated in PCO specimens. MALAT1 was overexpressed in TGF-β2 induced LECs, and the knockdown of MALAT1 could attenuate TGF-β2 induced EMT. Besides, the upregulation of MALAT1 was correlated with the downregulation of miR-204-5p and upregulation of Smad4. Importantly, MALAT1 was revealed to be located in the cytoplasm of LECs. Furthermore, luciferase reporter assays confirmed that MALAT1 could negatively regulate the expression of miR-204-5p and then regulate its direct target Smad4. Finally, the knockdown of MALAT1 could inhibit the EMT, proliferation, and migration of LECs; however, those can be reversed by anti-miR-204-5p. Our findings reveal that MALAT1 may regulate EMT, proliferation, and migration of LECs as a ceRNA by “sponging” miR-204-5p and targeting Smad4, and serve as a promising therapeutic target in preventing PCO.
DOI: 10.6084/m9.figshare.14038378
title: Ocular surface response of two preservative-free cylcosporine A emulsion eye drops in a mouse model of dry eye

description: Dry eye (DE) disease is a multifactorial disease in which uncontrolled inflammation can lead to corneal epithelium lesions and symptoms of discomfort. The aim of the present study was to evaluate the efficacy of two cyclosporine emulsions in a mouse model of DE with corneal epithelium lesions. Six- to 9-week-old female C57BL/6 N mice were housed in a controlled-environment room to induce DE. Following DE development, mice were instilled with: QD 0.1%CsA cationic emulsion (CaEm), BID 0.05%CsA anionic emulsion (AEm), or left untreated. Aqueous tear production and corneal epithelium lesions were assessed throughout the experiment. At the end of the treatment period, left eyes were sampled, fixed, and stained for histology, while the cornea, conjunctiva, and lacrimal gland of right eyes were sampled for transcriptomic analysis. Corneal lesion scores were reduced by 10.4%, 18.4%, and 10.9% at day 6, 10, and 14, respectively, with CaEm (QD), and by 2.6%, 3.0%, and 5.5% at day 6, 10, and 14, respectively, with AEm (BID). Histology demonstrated that 7 out of 10 DE mice presented moderate to severe ocular lesions, while only 2 and 5 out of 10 mice presented slight to moderate ocular lesions when treated with the CaEm (QD) and AEm (BID), respectively. The transcriptomic profile analysis suggests that a different set of inflammatory genes are modulated in the cornea, conjunctiva, and lacrimal gland upon DE development. In addition, the two emulsions distinctively modulate the gene expression profile. This study demonstrates that both emulsions were effective at reducing corneal lesions, with the CaEm (QD) being slightly better than the AEm (BID). Interestingly, this study suggests that ocular tissues may not respond similarly to a dry environment and that a different set of genes is modulated by the two formulations in the ocular tissues.
DOI: 10.6084/m9.figshare.14038378.v1
title: Ocular surface response of two preservative-free cylcosporine A emulsion eye drops in a mouse model of dry eye

description: Dry eye (DE) disease is a multifactorial disease in which uncontrolled inflammation can lead to corneal epithelium lesions and symptoms of discomfort. The aim of the present study was to evaluate the efficacy of two cyclosporine emulsions in a mouse model of DE with corneal epithelium lesions. Six- to 9-week-old female C57BL/6 N mice were housed in a controlled-environment room to induce DE. Following DE development, mice were instilled with: QD 0.1%CsA cationic emulsion (CaEm), BID 0.05%CsA anionic emulsion (AEm), or left untreated. Aqueous tear production and corneal epithelium lesions were assessed throughout the experiment. At the end of the treatment period, left eyes were sampled, fixed, and stained for histology, while the cornea, conjunctiva, and lacrimal gland of right eyes were sampled for transcriptomic analysis. Corneal lesion scores were reduced by 10.4%, 18.4%, and 10.9% at day 6, 10, and 14, respectively, with CaEm (QD), and by 2.6%, 3.0%, and 5.5% at day 6, 10, and 14, respectively, with AEm (BID). Histology demonstrated that 7 out of 10 DE mice presented moderate to severe ocular lesions, while only 2 and 5 out of 10 mice presented slight to moderate ocular lesions when treated with the CaEm (QD) and AEm (BID), respectively. The transcriptomic profile analysis suggests that a different set of inflammatory genes are modulated in the cornea, conjunctiva, and lacrimal gland upon DE development. In addition, the two emulsions distinctively modulate the gene expression profile. This study demonstrates that both emulsions were effective at reducing corneal lesions, with the CaEm (QD) being slightly better than the AEm (BID). Interestingly, this study suggests that ocular tissues may not respond similarly to a dry environment and that a different set of genes is modulated by the two formulations in the ocular tissues.
DOI: 10.6084/m9.figshare.13468625
title: A Single Low-Dose of Methylphenidate Improves Abnormal Visual Field Testing

description: To evaluate the effect of methylphenidate on visual field testing in healthy adults with abnormal visual field results. This prospective, randomized, controlled interventional clinical trial comprised all patients who had abnormal visual field test results and normal eye examination and ophthalmic history. Eligible patients were randomly assigned to either the study group or the control group. All patients repeated their visual field testing. Study group patients received a single dose of 10 mg methylphenidate prior to that. The main outcome measures were the percent difference in mean deviation and pattern standard deviation between the second and first visual fields. The methylphenidate group had greater improvement in all parameters. Mean deviation improved by median 68% (IQR 19%–78%) in the methylphenidate group vs. 27% [−5% to 55%] in the controls. However, this was not statistically significant (<i>p</i> = .83). Pattern standard deviation improved by median 49% (22%–59%) vs. 7% [−9% to 45%], respectively (<i>p</i> = .012). The visual fields were also reviewed by 3 masked experienced ophthalmologists. They indicated that the second visual field improved in 76.2% of the methylphenidate group vs. 48.5% of the controls (<i>p</i> = .04). A normal repeat visual field occurred in 57.7% vs. 21.2%, respectively. A subgroup analysis of patients with prior experience in visual field testing yielded an even more striking improvement in the methylphenidate group vs. controls. A single low dose of methylphenidate can improve visual field testing in subjects without ocular pathology, and even more in those with prior experience in perimetry.
DOI: 10.6084/m9.figshare.13468625.v1
title: A Single Low-Dose of Methylphenidate Improves Abnormal Visual Field Testing

description: To evaluate the effect of methylphenidate on visual field testing in healthy adults with abnormal visual field results. This prospective, randomized, controlled interventional clinical trial comprised all patients who had abnormal visual field test results and normal eye examination and ophthalmic history. Eligible patients were randomly assigned to either the study group or the control group. All patients repeated their visual field testing. Study group patients received a single dose of 10 mg methylphenidate prior to that. The main outcome measures were the percent difference in mean deviation and pattern standard deviation between the second and first visual fields. The methylphenidate group had greater improvement in all parameters. Mean deviation improved by median 68% (IQR 19%–78%) in the methylphenidate group vs. 27% [−5% to 55%] in the controls. However, this was not statistically significant (<i>p</i> = .83). Pattern standard deviation improved by median 49% (22%–59%) vs. 7% [−9% to 45%], respectively (<i>p</i> = .012). The visual fields were also reviewed by 3 masked experienced ophthalmologists. They indicated that the second visual field improved in 76.2% of the methylphenidate group vs. 48.5% of the controls (<i>p</i> = .04). A normal repeat visual field occurred in 57.7% vs. 21.2%, respectively. A subgroup analysis of patients with prior experience in visual field testing yielded an even more striking improvement in the methylphenidate group vs. controls. A single low dose of methylphenidate can improve visual field testing in subjects without ocular pathology, and even more in those with prior experience in perimetry.
DOI: 10.15468/dl.7ctryq
title: Occurrence Download

description: A dataset containing 17878 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is South America",
 "Country is Colombia",
 "GadmGid is COL.13.26_2",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 17878 records from 122 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009790-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.18770998
title: LINF_340008000

description: Vacuolar fusion protein Mon1-like protein; Leishmania infantum (strain JPCM5)
DOI: 10.5281/zenodo.18770997
title: LINF_340008000

description: Vacuolar fusion protein Mon1-like protein; Leishmania infantum (strain JPCM5)
DOI: 10.15468/dl.6bnevm
title: Occurrence Download

description: A dataset containing 8736 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Trichilia hirta",
 "Country is Colombia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 8736 records from 72 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009791-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25824/redu/xy7vmc
title: Dados experimentais associados ao desenvolvimento de técnicas de descelularização supercrítica de cartilagem auricular porcina para obtenção de materiais com aplicação no desenvolvimento de hidrogeis e scaffolds para medicina regenerativa

description: Dados mecânicos e de concentração de DNA de cartilagens auriculares porcinas submetidas ao processo de extração supercrítica, com diferentes tratamentos e cosolventes, para sua desceluarização. O objetivo é avaliar os efeitos dessas técnicas nas características mecânicas e morfológicas, visando a aplicação do material para fabricação de hidrogéis e scaffolds em engenharia tecidual.
DOI: 10.15468/dl.4bn5rc
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Platygaster zosine",
 "BasisOfRecord is Human Observation",
 "Year is greater than or equal to 1990",
 "Year is less than or equal to 2026"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009793-260221153910048/datasets/export for details.
DOI: 10.6084/m9.figshare.31396518
title: YouTube Travel Videos Metadata: Verified Authority Ledger & AI Routing Taxonomy

description: This dataset serves as the central Authority Ledger and metadata index for 2,267 travel-related YouTube videos produced by the Samuel & Audrey Media Network. Acting as the critical "directory layer," it links the creator's raw video assets to their corresponding transcript corpora (both English-only and bilingual parallel datasets).Explicitly engineered for Answer Engine Optimization (AEO) and AI routing, this dataset provides verified videoIds, geographic regional tags, view counts, and publication years. This structured taxonomy enables AI models to perform accurate entity resolution, temporal filtering, and geographic routing before initiating text retrieval. Furthermore, this dataset integrates comprehensive metadata frameworks—including precise <code>ImageObject</code> schema mapping to properly structure visual assets for knowledge graphs—ensuring high-fidelity verification and cryptographic ownership across enterprise RAG systems.
DOI: 10.6084/m9.figshare.31396518.v1
title: YouTube Travel Videos Metadata: Verified Authority Ledger & AI Routing Taxonomy

description: This dataset serves as the central Authority Ledger and metadata index for 2,267 travel-related YouTube videos produced by the Samuel & Audrey Media Network. Acting as the critical "directory layer," it links the creator's raw video assets to their corresponding transcript corpora (both English-only and bilingual parallel datasets).Explicitly engineered for Answer Engine Optimization (AEO) and AI routing, this dataset provides verified videoIds, geographic regional tags, view counts, and publication years. This structured taxonomy enables AI models to perform accurate entity resolution, temporal filtering, and geographic routing before initiating text retrieval. Furthermore, this dataset integrates comprehensive metadata frameworks—including precise <code>ImageObject</code> schema mapping to properly structure visual assets for knowledge graphs—ensuring high-fidelity verification and cryptographic ownership across enterprise RAG systems.
DOI: 10.15468/dl.ndnzm5
title: Occurrence Download

description: A dataset containing 1684 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lynx pardinus",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 1684 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009795-260221153910048/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.