cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7515614
title: Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques

description: Sclerotinia sclerotiorum, the etiological agent of the "white mould" in soybean, is responsible for severe losses in this crop and soil contamination. The introduction and dissemination of the disease can made through the use of seed lots contaminated with sclerotia and by seeds infected by mycelium. Therefore, seed health quality is one aspect to be monitored by means of health testing before to sowing time. In this study conventional and quantitative PCR techniques were used to assess their viability to detect S. sclerotiorum in artificially and naturally infected soybean seed samples. For that, seeds were inoculated by osmotic conditioning technique for 0, 24, 48 and 72 hours of contact of the seed with the fungal colony and mixed with healthy seeds generating incidence levels of 1, 2, 10, 20 and 100% for each incubation time. The cPCR was sensitive to detect S. sclerotiorum in samples with at least incidence 1% inoculated for 72 hours while the qPCR detected the pathogen in all incidence/inoculum potential combinations. The conventional PCR was able to detect 0.25% of the incidence of S. sclerotiorum in soybean seed lots naturally infected added a preincubation step.
DOI: 10.6084/m9.figshare.7515614.v1
title: Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques

description: Sclerotinia sclerotiorum, the etiological agent of the "white mould" in soybean, is responsible for severe losses in this crop and soil contamination. The introduction and dissemination of the disease can made through the use of seed lots contaminated with sclerotia and by seeds infected by mycelium. Therefore, seed health quality is one aspect to be monitored by means of health testing before to sowing time. In this study conventional and quantitative PCR techniques were used to assess their viability to detect S. sclerotiorum in artificially and naturally infected soybean seed samples. For that, seeds were inoculated by osmotic conditioning technique for 0, 24, 48 and 72 hours of contact of the seed with the fungal colony and mixed with healthy seeds generating incidence levels of 1, 2, 10, 20 and 100% for each incubation time. The cPCR was sensitive to detect S. sclerotiorum in samples with at least incidence 1% inoculated for 72 hours while the qPCR detected the pathogen in all incidence/inoculum potential combinations. The conventional PCR was able to detect 0.25% of the incidence of S. sclerotiorum in soybean seed lots naturally infected added a preincubation step.
DOI: 10.6084/m9.figshare.7516436
title: Seasonal dynamics of nutrients in an Amazon estuary

description: AbstractThis paper shows the nutrients distribution in the Paracauari estuary over a Amazon hydrological cycle (2008), and at the end of the period of La Niña (April 2008). This estuary is influenced by the humid tropical climate and meso-tidal (3 to 4 m), semi-diurnal. Sampling was conducted at 10 stations in three distinct seasons: rainy (march), intermediate (june) and less rainy season (september). Were measured in situ physical and chemical parameters using a multiparameter probe; analyzed the dissolved nutrients (nitrate, nitrite, ammonia- -N, phosphate and silicate) by spectrophotometry and particulate matter suspension, gravimetrically. We observed large seasonal variations in the concentrations of the studied parameters. The water temperature (average of 28.58 °C) is quite homogeneous, typical of tropical waters. The pH ranged acid (5.80) to alkaline (7.86) and salinity between 0.06 to 7.56, with both maximum values at the mouth because of the greater marine influence. The waters are poorly oxygenated during the rainy season (2.35 mg.L-1) and at the very least rainy (6.55 mg.L-1). The concentrations of the suspended particulate matter and nutrients reached a maximum during the rainy season due to the natural inflow from adjacent areas. In this estuary, the nutrients are mainly controlled by the river discharge, rainfall, wind and tide and exceptionally by la Niña.
DOI: 10.6084/m9.figshare.7514411.v1
title: Evaluation of Sustainability of Brazilian Ethanol Production: A model in System Dynamics

description: ABSTRACT System dynamics is an approach to analyze the behavior of complex systems, such as the productive chains, strictly considering the inherent characteristics. This approach is based on mathematical concepts of nonlinear processes developed in mathematics and physics and consolidated in engineering. The concepts inherent in this approach assists in creating a mathematical model that represents a production chain by using computer simulation. Thus, the main objective of this paper is to present the formalization of the dynamic model of assessing the sustainability of Brazilian ethanol production. We analyzed the external environment and the scenarios needed for a deeper understanding of relation of cause and effect, causal loops and diagrams of flows and stocks because of the awareness stage, with regard to understanding the problems involved, according to methodology known as design science.
DOI: 10.6084/m9.figshare.7517951
title: Larvae of Nile tilapia lines subject to feeding frequencies under low temperature

description: SUMMARY The adequate food management associated with animals with higher yields and resistance to specific environments, such as waters with temperatures below the appropriate temperature, are determinant for a higher productivity. Therefore, the aim of this study was to compare larviculture performance of two strains of Nile tilapia (Oreochromis niloticus) submitted to different feeding frequencies with temperature below the optimum range for growth. Larvae of Thai (720 individuals with weight of 24.3 mg and total length of 12.4 mm) and GIFT strains (720 individuals with weight of 22.5 mg and total length of 12.0 mm) were distributed into 24 aquariuns-4L, the density of 15 larvae. L−1, 60 larvae aquarium−1, under constant aeration, natural photoperiod and temperature of 23 °C, with recirculation and thermostat. The fish were subjected to three feeding frequencies: 2, 5 and 8 times.day−1 in a randomized design in a 2 strains x 3 frequencies factorial scheme, with four replicates each. Except the temperature the other parameters of water quality, ammonia, nitrite, nitrate, turbidity, conductivity and pH, remained adequate for the species. The Thai lineage showed higher total and standard length, weight, Fulton condition factor, survival and biomass, than GIFT. The feeding frequency 5 times.day-1 led to higher values larvae in weight, standard and total length in relation the frequency of 2 times.day-1 but, when compared values of frequency 8 times.day-1 was not difference. In the temperature 23 oC, Thai strain fed 5 times.day-1 should be used.
DOI: 10.6084/m9.figshare.7511636
title: CLINICAL, HEMATOLOGICAL, AND SEMINAL ALTERATIONS AND PARASITEMIA OF MALE GOATS EXPERIMENTALLY INFECTED WITH Toxoplasma gondii

description: Abstract:Toxoplasmosis is a parasitic disease that affects reproductive performance in small ruminants. Although the Toxoplasma gondii life cycle is well understood since 1960s, several aspects related to its infection remains unclear. This study aimed to determine the effects of T. gondiiexperimental infection, and the influence on clinical, hematological, parasitemia and seminal parameters in male goats. Nine animals were selected and distributed in three groups: GI (n=3) – control group (placebo) orally inoculated with saline solution; GII (n=3) – subcutaneously inoculated with 1 x 106 tachyzoites of T. gondii; and GIII (n=3) – orally inoculated with 2 x 10<sup>5</sup> oocysts of T. gondii. After that, clinical exams, serological tests, hemograms, parasitemia determination and semen evaluation were performed. Reciprocal serological titers had highest values of 4096 in both groups of goats infected with T. gondii,confirming the experimental infections. However, we could not observe clinical changes (except for mild hyperthermia on the 5<sup>th</sup> DAI in one of the animals - GIII) or in hematimetric parameters. Although there were some statistically significant changes (P &lt;0.05) on the percentages of pathology and sperm concentrations in some of the dates between the infected and control animals, these changes were not associated with toxoplasmic infection. Infection was associated with animal handling methods and environmental factors.
DOI: 10.6084/m9.figshare.7511636.v1
title: CLINICAL, HEMATOLOGICAL, AND SEMINAL ALTERATIONS AND PARASITEMIA OF MALE GOATS EXPERIMENTALLY INFECTED WITH Toxoplasma gondii

description: Abstract:Toxoplasmosis is a parasitic disease that affects reproductive performance in small ruminants. Although the Toxoplasma gondii life cycle is well understood since 1960s, several aspects related to its infection remains unclear. This study aimed to determine the effects of T. gondiiexperimental infection, and the influence on clinical, hematological, parasitemia and seminal parameters in male goats. Nine animals were selected and distributed in three groups: GI (n=3) – control group (placebo) orally inoculated with saline solution; GII (n=3) – subcutaneously inoculated with 1 x 106 tachyzoites of T. gondii; and GIII (n=3) – orally inoculated with 2 x 10<sup>5</sup> oocysts of T. gondii. After that, clinical exams, serological tests, hemograms, parasitemia determination and semen evaluation were performed. Reciprocal serological titers had highest values of 4096 in both groups of goats infected with T. gondii,confirming the experimental infections. However, we could not observe clinical changes (except for mild hyperthermia on the 5<sup>th</sup> DAI in one of the animals - GIII) or in hematimetric parameters. Although there were some statistically significant changes (P &lt;0.05) on the percentages of pathology and sperm concentrations in some of the dates between the infected and control animals, these changes were not associated with toxoplasmic infection. Infection was associated with animal handling methods and environmental factors.
DOI: 10.6084/m9.figshare.7516436.v1
title: Seasonal dynamics of nutrients in an Amazon estuary

description: AbstractThis paper shows the nutrients distribution in the Paracauari estuary over a Amazon hydrological cycle (2008), and at the end of the period of La Niña (April 2008). This estuary is influenced by the humid tropical climate and meso-tidal (3 to 4 m), semi-diurnal. Sampling was conducted at 10 stations in three distinct seasons: rainy (march), intermediate (june) and less rainy season (september). Were measured in situ physical and chemical parameters using a multiparameter probe; analyzed the dissolved nutrients (nitrate, nitrite, ammonia- -N, phosphate and silicate) by spectrophotometry and particulate matter suspension, gravimetrically. We observed large seasonal variations in the concentrations of the studied parameters. The water temperature (average of 28.58 °C) is quite homogeneous, typical of tropical waters. The pH ranged acid (5.80) to alkaline (7.86) and salinity between 0.06 to 7.56, with both maximum values at the mouth because of the greater marine influence. The waters are poorly oxygenated during the rainy season (2.35 mg.L-1) and at the very least rainy (6.55 mg.L-1). The concentrations of the suspended particulate matter and nutrients reached a maximum during the rainy season due to the natural inflow from adjacent areas. In this estuary, the nutrients are mainly controlled by the river discharge, rainfall, wind and tide and exceptionally by la Niña.
DOI: 10.6084/m9.figshare.7517951.v1
title: Larvae of Nile tilapia lines subject to feeding frequencies under low temperature

description: SUMMARY The adequate food management associated with animals with higher yields and resistance to specific environments, such as waters with temperatures below the appropriate temperature, are determinant for a higher productivity. Therefore, the aim of this study was to compare larviculture performance of two strains of Nile tilapia (Oreochromis niloticus) submitted to different feeding frequencies with temperature below the optimum range for growth. Larvae of Thai (720 individuals with weight of 24.3 mg and total length of 12.4 mm) and GIFT strains (720 individuals with weight of 22.5 mg and total length of 12.0 mm) were distributed into 24 aquariuns-4L, the density of 15 larvae. L−1, 60 larvae aquarium−1, under constant aeration, natural photoperiod and temperature of 23 °C, with recirculation and thermostat. The fish were subjected to three feeding frequencies: 2, 5 and 8 times.day−1 in a randomized design in a 2 strains x 3 frequencies factorial scheme, with four replicates each. Except the temperature the other parameters of water quality, ammonia, nitrite, nitrate, turbidity, conductivity and pH, remained adequate for the species. The Thai lineage showed higher total and standard length, weight, Fulton condition factor, survival and biomass, than GIFT. The feeding frequency 5 times.day-1 led to higher values larvae in weight, standard and total length in relation the frequency of 2 times.day-1 but, when compared values of frequency 8 times.day-1 was not difference. In the temperature 23 oC, Thai strain fed 5 times.day-1 should be used.
DOI: 10.6084/m9.figshare.8127788
title: Preoperative detection of TERT promoter and BRAFV600E mutations in papillary thyroid carcinoma in high-risk thyroid nodules

description: ABSTRACT Objectives: This observational study analyzed telomerase reverse transcriptase (pTERT) mutations in 45 fine-needle aspiration (FNA) specimens obtained from thyroid nodules followed by postoperatively confirmation of papillary thyroid cancer (PTC) diagnosis, examining their relationship with clinicopathologic aspects and the BRAFV600E mutation. Subjects and methods: Clinical information was collected from patients who presented to Ribeirao Preto University Hospital for surgical consultation regarding a thyroid nodule and who underwent molecular testing between January 2010 to October 2012. Tests included a DNA-based somatic detection of BRAFV600E and pTERT mutations. Results: We found coexistence of pTERTC228T and BRAFV600E mutations in 8.9% (4/45) of thyroid nodules. All nodules positive for pTERT mutations were BRAFV600E positives. There was a significant association between pTERTC228T/BRAFV600E with older age and advanced stage compared with the group negative for either mutation. Conclusions: This series provides evidence that FNA is a reliable method for preoperative diagnosis of high-risk thyroid nodules. pTERTC228T/BRAFV600E mutations could be a marker of poor prognosis. Its use as a personalized molecular medicine tool to individualize treatment decisions and follow-up design needs to be further studied.
DOI: 10.6084/m9.figshare.5791902
title: Antibacterial effectiveness in vitro of different formulations of calcium hydroxide paste

description: ABSTRACT Objective: To evaluate the antibacterial activity of four formulations of calcium hydroxide paste against microorganisms commonly found in infected root canals. Methods: To evaluate antibacterial activity through the agar diffusion method, pastes of calcium hydroxide were made from its pro-analysis form, diffused into four separate vehicles: distilled water, camphorated p-monochlorophenol, propylene glycol and Otosporin®, testing the antimicrobial activity of these on strains of Staphylococcus aureus, Bacillus subtilis and Enterococcus faecalis. After the incubation period, the presence or otherwise of inhibition zones were observed and their sizes in three stages: 24h, 48h and 72h. With this data, the median between the four dishes was obtained and the consequent value was submitted to Kruskal-Wallis nonparametric statistical analysis, with post-tests of Mann-Whitney and Bonferroni correction, at a significance level of 5%. Results: Only pastes with camphorated p-monochlorophenol and Otosporin vehicles caused the formation of significant inhibition zones, with medians of 8.0 mm. Against the strains of Enterococcus faecalis, only pastes with the camphorated p-monochlorophenol vehicle resulted in the formation of significant inhibition zones, with a median of 3.0 mm. Conclusion : Otosporin and CMCP vehicles provide greater antimicrobial potential to calcium hydroxide against the studied bacteria. However, only the Ca(OH)2 and CMCP combination was effective against all the strains, and can thus be regarded as the paste formulation with the greatest antimicrobial effectiveness in this study.
DOI: 10.6084/m9.figshare.5791902.v1
title: Antibacterial effectiveness in vitro of different formulations of calcium hydroxide paste

description: ABSTRACT Objective: To evaluate the antibacterial activity of four formulations of calcium hydroxide paste against microorganisms commonly found in infected root canals. Methods: To evaluate antibacterial activity through the agar diffusion method, pastes of calcium hydroxide were made from its pro-analysis form, diffused into four separate vehicles: distilled water, camphorated p-monochlorophenol, propylene glycol and Otosporin®, testing the antimicrobial activity of these on strains of Staphylococcus aureus, Bacillus subtilis and Enterococcus faecalis. After the incubation period, the presence or otherwise of inhibition zones were observed and their sizes in three stages: 24h, 48h and 72h. With this data, the median between the four dishes was obtained and the consequent value was submitted to Kruskal-Wallis nonparametric statistical analysis, with post-tests of Mann-Whitney and Bonferroni correction, at a significance level of 5%. Results: Only pastes with camphorated p-monochlorophenol and Otosporin vehicles caused the formation of significant inhibition zones, with medians of 8.0 mm. Against the strains of Enterococcus faecalis, only pastes with the camphorated p-monochlorophenol vehicle resulted in the formation of significant inhibition zones, with a median of 3.0 mm. Conclusion : Otosporin and CMCP vehicles provide greater antimicrobial potential to calcium hydroxide against the studied bacteria. However, only the Ca(OH)2 and CMCP combination was effective against all the strains, and can thus be regarded as the paste formulation with the greatest antimicrobial effectiveness in this study.
DOI: 10.6084/m9.figshare.7511684
title: Is 131I ablation necessary for patients with low-risk papillary thyroid carcinoma and slightly elevated stimulated thyroglobulin after thyroidectomy?

description: ABSTRACT Objective This prospective study evaluated the recurrence rate in low-risk patients with papillary thyroid cancer (PTC) who presented slightly elevated thyroglobulin (Tg) after thyroidectomy and who did not undergo ablation with131I. Subjects and methods The study included 53 low-risk patients (nonaggressive histology; pT1b-3, cN0pNx, M0) with slightly elevated Tg after thyroidectomy (&gt; 1 ng/mL, but ≤ 5 ng/mL after levothyroxine withdrawal or ≤ 2 ng/mL after recombinant human TSH). Results The time of follow-up ranged from 36 to 96 months. Lymph node metastases were detected in only one patient (1.9%). Fifty-two patients continued to present negative neck ultrasound. None of these patients without apparent disease presented an increase in Tg. Conclusions Low-risk patients with PTC who present slightly elevated Tg after thyroidectomy do not require ablation with 131I.
DOI: 10.6084/m9.figshare.7511705.v1
title: Is radioactive iodine- 131 treatment related to the occurrence of non-synchronous second primary malignancy in patients with differentiated thyroid cancer?

description: Objective Much controversy relates to the risk of non-synchronous second primary malignancies (NSSPM) after radioactive iodine treatment (RAI-131) in differentiated thyroid cancer (DTC) patients. This study evaluated the relationship between RAI-131 and NSSPM in DTC survivors with long-term follow-up. Materials and methods Retrospective analysis of 413 DTC cases was performed; 252 received RAI-131 and 161 were treated with thyroidectomy alone. Exclusion criteria were: prior or synchronous non-thyroidal malignancies (within the first year), familial syndromes associated to multiple neoplasms, ionizing radiation exposure or second tumors with unknown histopathology. Results During a mean follow-up of 11.0 ± 7.5 years, 17 (4.1%) patients developed solid NSSPM. Patients with NSSPM were older than those without (p = 0.02). RAI-131 and I-131 cumulative activity were similar in patients with and without NSSPM (p = 0.18 and p = 0.78, respectively). Incidence of NSSPM was 5.2% in patients with RAI-131 treatment and 2.5% in those without RAI-131 (p = 0.18). Using multivariate analysis, RAI-131 was not significantly associated with NSSPM occurrence (p = 0.35); age was the only independent predictor (p = 0.04). Under log rank statistical analysis, after 10 years of follow-up, it was observed a tendency of lower NSSPM-free survival among patients that received RAI-131 treatment (0.96 vs . 0.87; p = 0.06), what was not affected by age at DTC diagnosis. Conclusion In our cohort of DTC survivors, with a long-term follow-up period, RAI-131 treatment and I-131 cumulative dose were not significantly associated with NSSPM occurrence. A tendency of premature NSSPM occurrence among patients treated with RAI-131 was observed, suggesting an anticipating oncogenic effect by interaction with other risk factors.
DOI: 10.6084/m9.figshare.5792376
title: Evaluation of the tip of standardized D0 gutta percha cones of four Rotary systems, by means of an endodontic ruler

description: ABSTRACT Objective : This study aimed to evaluate the D0 diameter of standardized gutta-percha cones for four mechanized systems: ProTaper Universal®, Mtwo®, Wave One® and Reciproc®. Methods: An endodontic calibrator ruler was used to measure the D0 diameter of 60 main cones of the above systems. Measurements were made according 3 scores as follows: gutta-percha cone fits exactly into the same hole as the endodontic ruler gauge (score 1), cone falls short of the ruler gauge whole size (score 2); or exceeding the ruler gauge hole size (score 3). The diameters D0 obtained were compared with the values reported by manufacturers. All data were analyzed by means of T Test, at 1% level of significance. Results: The average value measured was significantly higher than the measures established by manufacturers (p &lt;0.001). Conclusion : The gutta-percha cones of ProTaper Universal® systems Mtwo®, Wave One® and Reciproc® were not standardized, except for R40 cone of the Reciproc® system (Reciproc®). The trend was for variation in the D0 Diameter towards increasing measurement values.
DOI: 10.6084/m9.figshare.7511684.v1
title: Is 131I ablation necessary for patients with low-risk papillary thyroid carcinoma and slightly elevated stimulated thyroglobulin after thyroidectomy?

description: ABSTRACT Objective This prospective study evaluated the recurrence rate in low-risk patients with papillary thyroid cancer (PTC) who presented slightly elevated thyroglobulin (Tg) after thyroidectomy and who did not undergo ablation with131I. Subjects and methods The study included 53 low-risk patients (nonaggressive histology; pT1b-3, cN0pNx, M0) with slightly elevated Tg after thyroidectomy (&gt; 1 ng/mL, but ≤ 5 ng/mL after levothyroxine withdrawal or ≤ 2 ng/mL after recombinant human TSH). Results The time of follow-up ranged from 36 to 96 months. Lymph node metastases were detected in only one patient (1.9%). Fifty-two patients continued to present negative neck ultrasound. None of these patients without apparent disease presented an increase in Tg. Conclusions Low-risk patients with PTC who present slightly elevated Tg after thyroidectomy do not require ablation with 131I.