cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ctvf3-yn313
title: MJ007 sacculi

description: sacculi purification of sporulating cells
description: <strong>Tilt Series Date:</strong> 2012-03-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kit Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> See E Tocheva Mol Micro 2013</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-43/rawdata/</p>mj28.mrc,  Tilt Series (Pixel Size 1.9 nm), 925.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-43/rawdata/mj28.mrc"
        > <i class="download icon"></i> Download </a></p>

        mj28_part112_2.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-11-43/3dimage_17662/mj28_part112_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vm71g-eyn21
title: Pseudoalteromonas Iuteoviolacea MP129

description: P Iuteo DvipABhcpI Dbact2 whole cells from liquid culture
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab, taken at Janelia Farms</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid culture</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
  mpa2013-07-12-18_slicer8616.jpg: Pseudoalteromonas iuteoviolacea .  Chemoreceptor mpa2013-07-12-18_slicer8617.jpg: Pseudoalteromonas iuteoviolacea .  Unknown appendage
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-18/rawdata/</p>2013_07_12_JF_MP129_b_0013.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-18/rawdata/2013_07_12_JF_MP129_b_0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_07_12_JF_MP129_b_0013_2k_full.rec,  Reconstruction (Pixel Size 1.68 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-18/3dimage_28950/2013_07_12_JF_MP129_b_0013_2k_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kzfbt-ptb37
title: gold labeled CheA- in vitro

description: post wash sample - gold labeled CheA, 1.8 nm gold, post ternary formation snap rxn.
description: <strong>Tilt Series Date:</strong> 2013-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Heather Hodge and Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Buffer: 1x Snap + 1mM DTT 50 mM Tris Hcl pH 7.5, 100 mM Ncl, 0.1% Tween-200)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-79/rawdata/</p>HTH1B_10033.mrc,  Tilt Series (Pixel Size 0.2989 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-79/rawdata/HTH1B_10033.mrc"
        > <i class="download icon"></i> Download </a></p>

        HTH1B_10033_part67_6.rec,  Reconstruction (Pixel Size 0.5978 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-79/3dimage_26522/HTH1B_10033_part67_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cjxp8-84k95
title: E. coli pED208

description: E. coli with plasmid pED208
description: <strong>Tilt Series Date:</strong> 2014-12-16</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells arrived dead.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-12/rawdata/</p>pED2080004.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-12/rawdata/pED2080004.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080004_full.rec,  Reconstruction (Pixel Size 2.52 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-12/3dimage_48800/pED2080004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/22v6j-xrt23
title: Nitrosopumilus maritimus

description: Nmar cells grown to mid log phase. Not concentrated, looking for cell division related CDV proteins. (Pipeline: autoprocref_1396627728)(Pipeline:  autoprocref_1397923498)
description: <strong>Tilt Series Date:</strong> 2014-05-10</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Nitrosopumilus maritimus</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> With David Stahl</p>
  rra2014-05-10-8_slicer3898.jpg:  .  [2014-05-12 16:30:50] nice DNA leaking out of cell rra2014-05-10-8_slicer3903.jpg: Cell lysis- DNA with histones? .  Nmar cells rra2014-05-10-8_slicer3904.jpg: Fusing vesicles? rra2014-05-10-8_slicer10487.jpg: Filament FTSz/CDV related?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-05-10-8/3dimage_42312/</p>nmar30001_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-05-10-8/3dimage_42312/nmar30001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        nmar30001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-05-10-8/rawdata/nmar30001.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jyyde-11n43
title: Cryo-sectioned synchronized Jurkat

description: Jurkats were cell-cycle synchronized (Sarah Cheng) using nocodazole then HP frozen and cryo-sectioned by Mark Ladinsky-Grid4
description: <strong>Tilt Series Date:</strong> 2012-08-16</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>
<strong>Strain:</strong> Clone E6-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cryo-sectioning by Mark Ladinsky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> nocodazole synchronized</p>
<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-08-16-1/3dimage_68651/</p>12aug16_mitoticjurkat_1_BINNED_full.rec,  Reconstruction (Pixel Size 2.0 nm), 535.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-08-16-1/3dimage_68651/12aug16_mitoticjurkat_1_BINNED_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12aug16_mitoticjurkat_1.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-08-16-1/rawdata/12aug16_mitoticjurkat_1.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wc88w-h6j97
title: UU 2803- A413T delta P1P2

description: E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
description: <strong>Tilt Series Date:</strong> 2012-09-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown in TB to log phase, then lysed with Penicillin for one hour</p>
<strong>Sample Preparation:</strong> cells plunge frozen on R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-1/rawdata/</p>Coli2803atlas0917_10001.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-1/rawdata/Coli2803atlas0917_10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Coli2803atlas0917_10001_part130_8.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-1/3dimage_20808/Coli2803atlas0917_10001_part130_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nyy3a-mk698
title: HIV capsid

description: Box C, 0.5 exposure, 152, 41000 mag 3 defocus, slit 20 eV, search track 13500
description: <strong>Tilt Series Date:</strong> 2013-12-05</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 152.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod, need to reconstruct with SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified HIV cores</p>
<strong>Sample Preparation:</strong> Box C</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-36/rawdata/</p>hivcapsid00004.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-36/rawdata/hivcapsid00004.mrc"
        > <i class="download icon"></i> Download </a></p>

        hivcapsid00004_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-36/3dimage_35566/hivcapsid00004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/myfaz-9nq97
title: Agro Bald VirB8-GFP

description: the bald strain lacks flagellin 1-3 and is considered non-motile
description: <strong>Tilt Series Date:</strong> 2013-07-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown at 19C and induced for T4SS</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-12/rawdata/</p>bald_gfp20012.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-12/rawdata/bald_gfp20012.mrc"
        > <i class="download icon"></i> Download </a></p>

        bald_gfp20012_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-02-12/3dimage_27818/bald_gfp20012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ekmr3-vkt13
title: Streptococcus pneumoniae

description: over expression of SP_0107 PG hydroalse
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Streptococcus pneumoniae</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Andrew Camili</p>
<strong>Purification / Growth Conditions / Treatment:</strong> over expression of SP_0107 PG hydroalse</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-5/rawdata/</p>overexpr0005.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-5/rawdata/overexpr0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        overexpr0005_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-5/3dimage_30541/overexpr0005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xxy4g-0bz44
title: Treponema primitia Proteinase K

description: Raw data files of Treponema primitia Proteinase K
description: <strong>Tilt Series Date:</strong> 2012-04-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Treponema primitia</p>
<strong>Strain:</strong> ZAS-2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in the presence of proteinase K for 15min</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-27-2/rawdata/</p>protk0002.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-27-2/rawdata/protk0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        protk0002_full.rec,  Reconstruction (Pixel Size 3.8 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-27-2/3dimage_18302/protk0002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vgxbm-kjd78
title: E. coli N050

description: Sandwich fusion to sfGFP. Wild Type MreB (no point mutations)
description: <strong>Tilt Series Date:</strong> 2014-03-27</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli </p>
<strong>Strain:</strong> NO50</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Josh Schaevitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown at 37 to OD600 = 0.3 then plunge frozen.</p>
<strong>Sample Preparation:</strong> Plunge Frozen</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-03-27-7/rawdata/</p>032714_NO500007.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-03-27-7/rawdata/032714_NO500007.mrc"
        > <i class="download icon"></i> Download </a></p>

        032714_NO500007.rec,  Reconstruction (Pixel Size 0.96 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-03-27-7/3dimage_40547/032714_NO500007.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m7ef5-rxf28
title: MJ sacculi

description: Skinny B. subtilis MJ007 sacculi of sporulating cells
description: <strong>Tilt Series Date:</strong> 2012-03-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kit Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> see Mol Micro Tocheva et al reference</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-19/rawdata/</p>mjsac20019.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-19/rawdata/mjsac20019.mrc"
        > <i class="download icon"></i> Download </a></p>

        mjsac20019_part102_10.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-19/3dimage_17375/mjsac20019_part102_10.rec"
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DOI: 10.22002/mpnvc-hzg74
title: E. Coli delta53

description: Mutant MreB with sfGFP in sandwich position. WT MreB is replaced at the original locus with this fusion.
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia Coli </p>
<strong>Strain:</strong> delta53</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Josh Schaevitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 celsius to OD 600 ~ 0.3</p>
<strong>Sample Preparation:</strong> Blotted manually from rear in vitrobot at 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-01-52/rawdata/</p>040114_delta53_0022.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
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        040114_delta53_0022_part131_1.rec,  Reconstruction (Pixel Size 1.68 nm), 861.2 MB  
        
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