DOI: 10.5061/dryad.p1139
title: Data from: Enzyme polymorphism, oxygen and injury: a lipidomic analysis of flight-induced oxidative damage in a SDH-polymorphic insect

description: When active tissues receive insufficient oxygen to meet metabolic demand,
 succinate accumulates and has two fundamental effects: it causes
 ischemia-reperfusion injury while also activating the hypoxia-inducible
 factor pathway (HIF). The Glanville fritillary butterfly (Melitaea cinxia)
 possesses a balanced polymorphism in Sdhd, shown previously to affect HIF
 pathway activation and tracheal morphology and used here to experimentally
 test the hypothesis that variation in succinate dehydrogenase affects
 oxidative injury. We stimulated butterflies to fly continuously in a
 respirometer (3 min duration), which typically caused episodes of
 exhaustion and recovery, suggesting a potential for cellular injury from
 hypoxia and reoxygenation in flight muscles. Indeed, flight muscle from
 butterflies flown on consecutive days had lipidomic profiles similar to
 rested paraquat-injected butterflies, but distinct from rested untreated
 butterflies. Many butterflies showed a decline in flight metabolic rate
 (FMR) on Day 2, and there was a strong inverse relationship between the
 ratio of Day 2 to Day 1 FMR and the abundance of sodiated adducts of
 phosphatidylcholines and coenzyme Q (CoQ). This result is consistent with
 elevation of sodiated lipids caused by disrupted intracellular ion
 homeostasis in mammalian tissues after hypoxia-reperfusion. Butterflies
 carrying the Sdhd M allele had higher abundance of lipid markers of
 cellular damage, but the association was reversed in field-collected
 butterflies, where focal individuals typically flew for seconds at a time
 rather than continuously. These results indicate that Glanville fritillary
 flight muscles can be injured by episodes of high exertion, but injury
 severity appears to be determined by an interaction between SDH genotype
 and behavior (prolonged vs. intermittent flight).
description: Mono_Norm_Monoisotopic features from lipidomics analysis of flight muscle tissue from Glanville fritillary butterflies. Values are normalized by peak height of an internal standard (1 uM chloropropamide) and fresh mass of the individual tissue sample. Data are in folders named according to treatment (flown, rested, paraquat). File names indicate the ID number of each butterfly.Glanville fritillary lipidomics specimen dataExperimental data for each butterfly, with ID numbers in the column labeled "butterfly". These ID numbers correspond to the ID numbers in the file names of the lipidomics data files.Glanville_fritillary_lipidomics_specimen_data.xlsmark_recapture_lipidomics_all_dataThis is a single file containing butterfly data and all monoisotopic peak heights (normalized by the internal 1 uM chloropropamide spike-in standard) from samples of flight muscle. Columns at the end show peak heights (normalized also by fresh mass of tissue sample) of CoQ9 peaks.Mcinxia_Spain14_Field_FlightDurationsData from observations of focal individuals, comprising start and stop time for different behaviors, with durations noted for flights as well as distances (paces, estimated as 1m per pace) for relatively straight flights. Data include ambient temperatures from Hobo data loggers (two located 0.5m above the ground in full sun; two hung 1m above ground in shade).