DOI: 10.5061/dryad.71nv1
title: Data from: The genome of the clonal raider ant Cerapachys biroi

description: Social insects are important models for social evolution and behavior.
 However, in many species, experimental control over important factors that
 regulate division of labor, such as genotype and age, is limited.
 Furthermore, most species have fixed queen and worker castes, making it
 difficult to establish causality between the molecular mechanisms that
 underlie reproductive division of labor, the hallmark of insect societies.
 Here we present the genome of the queenless clonal raider ant Cerapachys
 biroi, a powerful new study system that does not suffer from these
 constraints. Using cytology and RAD-seq, we show that C. biroi reproduces
 via automixis with central fusion and that heterozygosity is lost
 extremely slowly. As a consequence, nestmates are almost clonally related
 (r = 0.996). Workers in C. biroi colonies synchronously alternate between
 reproduction and brood care, and young workers eclose in synchronized
 cohorts. We show that genes associated with division of labor in other
 social insects are conserved in C. biroi and dynamically regulated during
 the colony cycle. With unparalleled experimental control over an
 individual’s genotype and age, and the ability to induce reproduction and
 brood care, C. biroi has great potential to illuminate the molecular
 regulation of division of labor.
description: Gene details for
 Cerapachys biroi chemosensory genes, UGTs and CYPsTab 1 contains details
 of all manually annotated chemosensory genes from Cerapachys biroi. Tab 2
 contains all sequenced ant UDP glycosyltransferases, organised by clade
 (as described in supplemental data of Oxley et al.). Tab 3 contains all
 sequenced ant cytochrome P450 genes, organised by clade (as described in
 supplemental data of Oxley et al.).UGT CYP and OR gene
 lists.xlsxMUSCLE alignment of
 insect UDP glycosyltransferasesAlignment of UDP
 glycosyltransferases containing conserved signature motif (Mackenzie et
 al. Pharmacogenetics (1997) 7,255-269). Sequence includes motif and 100
 amino acids up- and downstream. Details can be found in supplemental data:
 UDP Glycosyltransferases (UGTs) in Oxley et al.UGTs_321.muscle.fastaNEXUS file of maximum likelihood tree for insect UGTsMaximum likelihood tree was constructed using Garli 2.0 (Poisson+G+I evolutionary model; best tree of five runs chosen), using the alignments provided in this repository.UGTs_321.treMUSCLE alignment of insect cytochrome P450 genesMUSCLE alignment of all sequenced ant CYPs and representative dipteran, hymenopteran and lepidopteran CYPs. All genes were manually checked for presence of conserved motifs (Nelson, D. Methods Mol. Biol. (2006) 320, 1-10). The alignment was further edited to remove the hypervariable non-conserved N-terminal up to (but not including) the N-terminal anchor sequence, as well as the region between the C-helix and I-helix. Further details can be found in Supplemental Data: Cytochrome P450 Genes of Oxley et al.CYPs_573.muscle.fastaNEXUS file of maximum likelihood tree for insect CYPsMaximum likelihood phylogeny was constructed using Garli 2.0 (Poisson+G+I evolutionary model; best tree of two runs chosen) using protein alignment provided in this repository. For further details see Supplemental Data: Cytochrome p450 Genes in Oxley et al.CYPs_573.tre