cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9750503
title: e-VOL BPMN: A Technique to Support the Evolution and Learning of BPMN Diagrams

description: In the software maintenance, business process models help practitioners understanding of the software, because it represents the organizational process in which software is embedded. BPMN (Business Process Modeling and Notation) is the standard notation for business process modeling. However, it is common for the BPMN diagrams to be outdated, which, among other possibilities, may be caused due to the lack of adequate knowledge of some practitioners about the notation. It is important to provide means to stimulate the evolution of these models and, consequently, to aid in the learning of BPMN notation. With this focus, we proposed and evaluated the e-VOL BPMN, a technique that supports the evolution of BPMN diagrams and on learning BPMN notation. In this evaluation, we compared e-VOL BPMN with the BPMN 2.0 Poster, an artifact used commonly to support BPMN notation in companies. Participants who used the e-VOL BPMN in a scenario of diagrams evolution presented more correct BPMN diagrams and had a higher learning perception of the notation. In addition, in a written exam on BPMN notation, participants who used e-VOL BPMN scored higher. From these results, we notice that e-VOL BPMN support both learning and evolution of BPMN diagrams.
DOI: 10.6084/m9.figshare.9750479
title: Supplementary Table - D. eleginoides

description: Annotation, Annotation by species, GO enrichment, KEGG enrichment, EST-SSRs, EST-SSRs primers<br>
DOI: 10.6084/m9.figshare.9750479.v1
title: Supplementary Table - D. eleginoides

description: Annotation, Annotation by species, GO enrichment, KEGG enrichment, EST-SSRs, EST-SSRs primers<br>
DOI: 10.6084/m9.figshare.9750569.v1
title: Motives behind Facebook usage: a study of 11587 users worldwide

description: <b>Marta Kowal<sup>1*</sup>, Piotr Sorokowski<sup>1</sup>, Agnieszka Sorokowska<sup>1</sup>, Małgorzata Dobrowolska<sup>2</sup>, Katarzyna Pisanski<sup>1</sup>, Anna Oleszkiewicz<sup>1, 51</sup>, Toivo Aavik<sup>3</sup>, Grace Akello<sup>5</sup>, Charlotte Alm<sup>6</sup>, Naumana Amjad<sup>7</sup>, Afifa Anjum<sup>7</sup>, Kelly Asao<sup>8</sup>, Chiemezie S. Atama<sup>9</sup>, Derya Atamtürk Duyar<sup>10</sup>, Richard Ayebare<sup>11</sup>, Mons Bendixen<sup>12</sup>, Aicha Bensafia<sup>15</sup>, Boris Bizumic<sup>14</sup>, Mahmoud Boussena<sup>15</sup>, David M. Buss<sup>8</sup>, Marina Butovskaya<sup>16</sup>, Seda Can<sup>17</sup>, Katarzyna Cantarero<sup>18</sup>, Antonin Carrier<sup>19</sup>, Hakan Cetinkaya<sup>17</sup>, Daniel Conroy-Beam<sup>20</sup>, Marco A. Correa Varella<sup>21</sup>, Rosa M. Cueto<sup>22</sup>, Marcin Czub<sup>1</sup>, Daria Dronova<sup>16</sup>, Seda Dural<sup>17</sup>, Izzet Duyar<sup>10</sup>, Berna Ertugrul<sup>10</sup>, Agustín Espinosa<sup>22</sup>, Ignacio Estevan<sup>23</sup>, Carla S. Esteves<sup>24</sup>, Tomasz Frąckowiak<sup>1</sup>, Jorge Contreras-Graduño<sup>25</sup>, Farida Guemaz<sup>13</sup>, Ivana Hromatko<sup>28</sup>, Chin-Ming Hui<sup>29</sup>, Herak Iskra<sup>19</sup>, Jas L. Jaafar<sup>30</sup>, Feng Jiang<sup>31</sup>, Konstantinos Kafetsios<sup>32</sup>, Tina Kavcic<sup>33</sup>, Leif Edward Ottesen Kennair<sup>12</sup>, Nicolas Kervyn<sup>19</sup>, Nils C. Köbis<sup>34</sup>, András Láng<sup>36</sup>, Georgina R. Lennard<sup>14</sup>, Ernesto León<sup>22</sup>, Torun Lindholm<sup>6</sup>, Giulia Lopez<sup>37</sup>, Mohammad Madallh Alhabahba<sup>38</sup>, Alvaro Mailhos<sup>23</sup>, Zoi Manesi<sup>34</sup>, Rocio Martinez<sup>39</sup>, Sarah L. McKerchar<sup>14</sup>, Norbert Meskó<sup>36</sup>, Girishwar Misra<sup>40</sup>, Hoang Moc Lan<sup>41</sup>, Conal Monaghan<sup>14</sup>, Emanuel C. Mora<sup>42</sup>, Alba Moya-Garofano<sup>39</sup>, Bojan Musil<sup>27</sup>, Jean C. Natividade<sup>43</sup>, George Nizharadze<sup>44</sup>, Elisabeth Oberzaucher<sup>45</sup>, Mohd S. Omar Fauzee<sup>46</sup>, Ernest Onyishi<sup>9</sup>, Baris Özener<sup>10</sup>, Ariela F. Pagani<sup>37</sup>, Vilmante Pakalniskiene<sup>47</sup>, Miriam Parise<sup>37</sup>, Farid Pazhoohi<sup>48</sup>, Mariia Perun<sup>49</sup>, Annette Pisanski<sup>42, 50</sup>, Nejc Plohl<sup>27</sup>, Camelia Popa<sup>21</sup>, Pavol Prokop<sup>52, 53</sup>, Muhammad Rizwan<sup>54</sup>, Mario Sainz<sup>4</sup>, Svjetlana Salkičević<sup>28</sup>, Ruta Sargautyte<sup>47</sup>, Susanne Schmehl<sup>45</sup>, Oksana Senyk<sup>49</sup>, Rizwana Shaikh<sup>55</sup>, Shivantika Sharad<sup>56</sup>, Franco Simonetti<sup>57</sup>, Meri Tadinac<sup>28</sup>, Truong Thi Khanh Ha<sup>41</sup>, Trinh Thi Linh<sup>41</sup>, Karina Ugalde González<sup>58</sup>, Nguyen Van Luot<sup>41</sup>, Christin-</b> <b>Melanie Vauclair<sup>24</sup>, Luis D. Vega Araya<sup>58</sup>, Gyesook Yoo<sup>60</sup>, Stanislava Yordanova Stoyanova<sup>26</sup>, Zainab F. Zadeh<sup>59</sup>, Maja Zupančič<sup>35</sup>.</b>
DOI: 10.6084/m9.figshare.9750503.v1
title: e-VOL BPMN: A Technique to Support the Evolution and Learning of BPMN Diagrams Systematic Mapping (Technical Report)

description: In the software maintenance, business process models help practitioners understanding of the software, because it represents the organizational process in which software is embedded. BPMN (Business Process Modeling and Notation) is the standard notation for business process modeling. However, it is common for the BPMN diagrams to be outdated, which, among other possibilities, may be caused due to the lack of adequate knowledge of some practitioners about the notation. It is important to provide means to stimulate the evolution of these models and, consequently, to aid in the learning of BPMN notation. With this focus, we proposed and evaluated the e-VOL BPMN, a technique that supports the evolution of BPMN diagrams and on learning BPMN notation. In this evaluation, we compared e-VOL BPMN with the BPMN 2.0 Poster, an artifact used commonly to support BPMN notation in companies. Participants who used the e-VOL BPMN in a scenario of diagrams evolution presented more correct BPMN diagrams and had a higher learning perception of the notation. In addition, in a written exam on BPMN notation, participants who used e-VOL BPMN scored higher. From these results, we notice that e-VOL BPMN support both learning and evolution of BPMN diagrams.
DOI: 10.6084/m9.figshare.9747911
title: Data set for study on intelligence and gender inequality

description: 89 countries
DOI: 10.6084/m9.figshare.9747860
title: Taxonomic affiliation of non-target bacterial sequences for each SAG. from Single Cell Ecogenomics reveals mating types of individual cells and ssDNA viral infections in the Smallest Photosynthetic Eukaryotes

description: Planktonic photosynthetic organisms of the class Mamiellophyceae include the smallest eukaryotes (less than 2 µm), are globally distributed and form the basis of coastal marine ecosystems. Eight complete fully annotated 13–22 Mb genomes from three genera, <i>Ostreococcus</i>, <i>Bathycoccus</i> and <i>Micromonas</i>, are available from previously isolated clonal cultured strains and provide an ideal resource to explore the scope and challenges of analysing Single Cell Amplified Genomes (SAGs) isolated from a natural environment. We assembled data from 12 SAGs sampled during the Tara Oceans expedition to gain biological insights about their <i>in situ</i> ecology, which might be lost by isolation and strain culture. Although the assembled nuclear genomes were incomplete, they were large enough to infer the mating types of four <i>Ostreococcus</i> SAGs. The systematic occurrence of sequences from the mitochondria and chloroplast, representing less than 3% of the total cell's DNA, intimates that SAGs provide suitable substrates for detection of non-target sequences, such as those of virions. Analysis of the non-Mamiellophyceae assemblies, following filtering out cross-contaminations during the sequencing process, revealed two novel 1.6 and 1.8 kb circular DNA viruses, and the presence of specific Bacterial and Oomycete sequences suggests that these organisms might co-occur with the Mamiellales.This article is part of the discussion meeting issue ‘Single cell ecology’.
DOI: 10.6084/m9.figshare.9750569.v2
title: Motives behind Facebook usage: a study of 11587 users worldwide

description: <b>Marta Kowal<sup>1*</sup>, Piotr Sorokowski<sup>1</sup>, Agnieszka Sorokowska<sup>1</sup>, Małgorzata Dobrowolska<sup>2</sup>, Katarzyna Pisanski<sup>1</sup>, Anna Oleszkiewicz<sup>1, 51</sup>, Toivo Aavik<sup>3</sup>, Grace Akello<sup>5</sup>, Charlotte Alm<sup>6</sup>, Naumana Amjad<sup>7</sup>, Afifa Anjum<sup>7</sup>, Kelly Asao<sup>8</sup>, Chiemezie S. Atama<sup>9</sup>, Derya Atamtürk Duyar<sup>10</sup>, Richard Ayebare<sup>11</sup>, Mons Bendixen<sup>12</sup>, Aicha Bensafia<sup>15</sup>, Boris Bizumic<sup>14</sup>, Mahmoud Boussena<sup>15</sup>, David M. Buss<sup>8</sup>, Marina Butovskaya<sup>16</sup>, Seda Can<sup>17</sup>, Katarzyna Cantarero<sup>18</sup>, Antonin Carrier<sup>19</sup>, Hakan Cetinkaya<sup>17</sup>, Daniel Conroy-Beam<sup>20</sup>, Marco A. Correa Varella<sup>21</sup>, Rosa M. Cueto<sup>22</sup>, Marcin Czub<sup>1</sup>, Daria Dronova<sup>16</sup>, Seda Dural<sup>17</sup>, Izzet Duyar<sup>10</sup>, Berna Ertugrul<sup>10</sup>, Agustín Espinosa<sup>22</sup>, Ignacio Estevan<sup>23</sup>, Carla S. Esteves<sup>24</sup>, Tomasz Frąckowiak<sup>1</sup>, Jorge Contreras-Graduño<sup>25</sup>, Farida Guemaz<sup>13</sup>, Ivana Hromatko<sup>28</sup>, Chin-Ming Hui<sup>29</sup>, Herak Iskra<sup>19</sup>, Jas L. Jaafar<sup>30</sup>, Feng Jiang<sup>31</sup>, Konstantinos Kafetsios<sup>32</sup>, Tina Kavcic<sup>33</sup>, Leif Edward Ottesen Kennair<sup>12</sup>, Nicolas Kervyn<sup>19</sup>, Nils C. Köbis<sup>34</sup>, András Láng<sup>36</sup>, Georgina R. Lennard<sup>14</sup>, Ernesto León<sup>22</sup>, Torun Lindholm<sup>6</sup>, Giulia Lopez<sup>37</sup>, Mohammad Madallh Alhabahba<sup>38</sup>, Alvaro Mailhos<sup>23</sup>, Zoi Manesi<sup>34</sup>, Rocio Martinez<sup>39</sup>, Sarah L. McKerchar<sup>14</sup>, Norbert Meskó<sup>36</sup>, Girishwar Misra<sup>40</sup>, Hoang Moc Lan<sup>41</sup>, Conal Monaghan<sup>14</sup>, Emanuel C. Mora<sup>42</sup>, Alba Moya-Garofano<sup>39</sup>, Bojan Musil<sup>27</sup>, Jean C. Natividade<sup>43</sup>, George Nizharadze<sup>44</sup>, Elisabeth Oberzaucher<sup>45</sup>, Mohd S. Omar Fauzee<sup>46</sup>, Ernest Onyishi<sup>9</sup>, Baris Özener<sup>10</sup>, Ariela F. Pagani<sup>37</sup>, Vilmante Pakalniskiene<sup>47</sup>, Miriam Parise<sup>37</sup>, Farid Pazhoohi<sup>48</sup>, Mariia Perun<sup>49</sup>, Annette Pisanski<sup>42, 50</sup>, Nejc Plohl<sup>27</sup>, Camelia Popa<sup>21</sup>, Pavol Prokop<sup>52, 53</sup>, Muhammad Rizwan<sup>54</sup>, Mario Sainz<sup>4</sup>, Svjetlana Salkičević<sup>28</sup>, Ruta Sargautyte<sup>47</sup>, Susanne Schmehl<sup>45</sup>, Oksana Senyk<sup>49</sup>, Rizwana Shaikh<sup>55</sup>, Shivantika Sharad<sup>56</sup>, Franco Simonetti<sup>57</sup>, Meri Tadinac<sup>28</sup>, Truong Thi Khanh Ha<sup>41</sup>, Trinh Thi Linh<sup>41</sup>, Karina Ugalde González<sup>58</sup>, Nguyen Van Luot<sup>41</sup>, Christin-</b> <b>Melanie Vauclair<sup>24</sup>, Luis D. Vega Araya<sup>58</sup>, Gyesook Yoo<sup>60</sup>, Stanislava Yordanova Stoyanova<sup>26</sup>, Zainab F. Zadeh<sup>59</sup>, Maja Zupančič<sup>35</sup>.</b>
DOI: 10.6084/m9.figshare.9747860.v1
title: Taxonomic affiliation of non-target bacterial sequences for each SAG. from Single Cell Ecogenomics reveals mating types of individual cells and ssDNA viral infections in the Smallest Photosynthetic Eukaryotes

description: Planktonic photosynthetic organisms of the class Mamiellophyceae include the smallest eukaryotes (less than 2 µm), are globally distributed and form the basis of coastal marine ecosystems. Eight complete fully annotated 13–22 Mb genomes from three genera, <i>Ostreococcus</i>, <i>Bathycoccus</i> and <i>Micromonas</i>, are available from previously isolated clonal cultured strains and provide an ideal resource to explore the scope and challenges of analysing Single Cell Amplified Genomes (SAGs) isolated from a natural environment. We assembled data from 12 SAGs sampled during the Tara Oceans expedition to gain biological insights about their <i>in situ</i> ecology, which might be lost by isolation and strain culture. Although the assembled nuclear genomes were incomplete, they were large enough to infer the mating types of four <i>Ostreococcus</i> SAGs. The systematic occurrence of sequences from the mitochondria and chloroplast, representing less than 3% of the total cell's DNA, intimates that SAGs provide suitable substrates for detection of non-target sequences, such as those of virions. Analysis of the non-Mamiellophyceae assemblies, following filtering out cross-contaminations during the sequencing process, revealed two novel 1.6 and 1.8 kb circular DNA viruses, and the presence of specific Bacterial and Oomycete sequences suggests that these organisms might co-occur with the Mamiellales.This article is part of the discussion meeting issue ‘Single cell ecology’.
DOI: 10.6084/m9.figshare.9750503.v2
title: e-VOL BPMN: A Technique to Support the Evolution and Learning of BPMN Diagrams Systematic Mapping (Technical Report)

description: In the software maintenance, business process models help practitioners understanding of the software, because it represents the organizational process in which software is embedded. BPMN (Business Process Modeling and Notation) is the standard notation for business process modeling. However, it is common for the BPMN diagrams to be outdated, which, among other possibilities, may be caused due to the lack of adequate knowledge of some practitioners about the notation. It is important to provide means to stimulate the evolution of these models and, consequently, to aid in the learning of BPMN notation. With this focus, we proposed and evaluated the e-VOL BPMN, a technique that supports the evolution of BPMN diagrams and on learning BPMN notation. In this evaluation, we compared e-VOL BPMN with the BPMN 2.0 Poster, an artifact used commonly to support BPMN notation in companies. Participants who used the e-VOL BPMN in a scenario of diagrams evolution presented more correct BPMN diagrams and had a higher learning perception of the notation. In addition, in a written exam on BPMN notation, participants who used e-VOL BPMN scored higher. From these results, we notice that e-VOL BPMN support both learning and evolution of BPMN diagrams.
DOI: 10.6084/m9.figshare.9747854
title: Genbank accession numbers of 18S sequences used for phylogeny. from Single Cell Ecogenomics reveals mating types of individual cells and ssDNA viral infections in the Smallest Photosynthetic Eukaryotes

description: Planktonic photosynthetic organisms of the class Mamiellophyceae include the smallest eukaryotes (less than 2 µm), are globally distributed and form the basis of coastal marine ecosystems. Eight complete fully annotated 13–22 Mb genomes from three genera, <i>Ostreococcus</i>, <i>Bathycoccus</i> and <i>Micromonas</i>, are available from previously isolated clonal cultured strains and provide an ideal resource to explore the scope and challenges of analysing Single Cell Amplified Genomes (SAGs) isolated from a natural environment. We assembled data from 12 SAGs sampled during the Tara Oceans expedition to gain biological insights about their <i>in situ</i> ecology, which might be lost by isolation and strain culture. Although the assembled nuclear genomes were incomplete, they were large enough to infer the mating types of four <i>Ostreococcus</i> SAGs. The systematic occurrence of sequences from the mitochondria and chloroplast, representing less than 3% of the total cell's DNA, intimates that SAGs provide suitable substrates for detection of non-target sequences, such as those of virions. Analysis of the non-Mamiellophyceae assemblies, following filtering out cross-contaminations during the sequencing process, revealed two novel 1.6 and 1.8 kb circular DNA viruses, and the presence of specific Bacterial and Oomycete sequences suggests that these organisms might co-occur with the Mamiellales.This article is part of the discussion meeting issue ‘Single cell ecology’.
DOI: 10.6084/m9.figshare.9747857
title: Plastid, mitochondrion and nuclear genome coverage in 12 Ostreococcus tauri strains and estimation of mitochondrial and plastid genome copy number per haploid nuclear genome. from Single Cell Ecogenomics reveals mating types of individual cells and ssDNA viral infections in the Smallest Photosynthetic Eukaryotes

description: Planktonic photosynthetic organisms of the class Mamiellophyceae include the smallest eukaryotes (less than 2 µm), are globally distributed and form the basis of coastal marine ecosystems. Eight complete fully annotated 13–22 Mb genomes from three genera, <i>Ostreococcus</i>, <i>Bathycoccus</i> and <i>Micromonas</i>, are available from previously isolated clonal cultured strains and provide an ideal resource to explore the scope and challenges of analysing Single Cell Amplified Genomes (SAGs) isolated from a natural environment. We assembled data from 12 SAGs sampled during the Tara Oceans expedition to gain biological insights about their <i>in situ</i> ecology, which might be lost by isolation and strain culture. Although the assembled nuclear genomes were incomplete, they were large enough to infer the mating types of four <i>Ostreococcus</i> SAGs. The systematic occurrence of sequences from the mitochondria and chloroplast, representing less than 3% of the total cell's DNA, intimates that SAGs provide suitable substrates for detection of non-target sequences, such as those of virions. Analysis of the non-Mamiellophyceae assemblies, following filtering out cross-contaminations during the sequencing process, revealed two novel 1.6 and 1.8 kb circular DNA viruses, and the presence of specific Bacterial and Oomycete sequences suggests that these organisms might co-occur with the Mamiellales.This article is part of the discussion meeting issue ‘Single cell ecology’.
DOI: 10.6084/m9.figshare.9750569.v3
title: Motives behind Facebook usage: a study of 11587 users worldwide

description: <b>Marta Kowal<sup>1*</sup>, Piotr Sorokowski<sup>1</sup>, Agnieszka Sorokowska<sup>1</sup>, Małgorzata Dobrowolska<sup>2</sup>, Katarzyna Pisanski<sup>1</sup>, Anna Oleszkiewicz<sup>1, 51</sup>, Toivo Aavik<sup>3</sup>, Grace Akello<sup>5</sup>, Charlotte Alm<sup>6</sup>, Naumana Amjad<sup>7</sup>, Afifa Anjum<sup>7</sup>, Kelly Asao<sup>8</sup>, Chiemezie S. Atama<sup>9</sup>, Derya Atamtürk Duyar<sup>10</sup>, Richard Ayebare<sup>11</sup>, Mons Bendixen<sup>12</sup>, Aicha Bensafia<sup>15</sup>, Boris Bizumic<sup>14</sup>, Mahmoud Boussena<sup>15</sup>, David M. Buss<sup>8</sup>, Marina Butovskaya<sup>16</sup>, Seda Can<sup>17</sup>, Katarzyna Cantarero<sup>18</sup>, Antonin Carrier<sup>19</sup>, Hakan Cetinkaya<sup>17</sup>, Daniel Conroy-Beam<sup>20</sup>, Marco A. Correa Varella<sup>21</sup>, Rosa M. Cueto<sup>22</sup>, Marcin Czub<sup>1</sup>, Daria Dronova<sup>16</sup>, Seda Dural<sup>17</sup>, Izzet Duyar<sup>10</sup>, Berna Ertugrul<sup>10</sup>, Agustín Espinosa<sup>22</sup>, Ignacio Estevan<sup>23</sup>, Carla S. Esteves<sup>24</sup>, Tomasz Frąckowiak<sup>1</sup>, Jorge Contreras-Graduño<sup>25</sup>, Farida Guemaz<sup>13</sup>, Ivana Hromatko<sup>28</sup>, Chin-Ming Hui<sup>29</sup>, Herak Iskra<sup>19</sup>, Jas L. Jaafar<sup>30</sup>, Feng Jiang<sup>31</sup>, Konstantinos Kafetsios<sup>32</sup>, Tina Kavcic<sup>33</sup>, Leif Edward Ottesen Kennair<sup>12</sup>, Nicolas Kervyn<sup>19</sup>, Nils C. Köbis<sup>34</sup>, András Láng<sup>36</sup>, Georgina R. Lennard<sup>14</sup>, Ernesto León<sup>22</sup>, Torun Lindholm<sup>6</sup>, Giulia Lopez<sup>37</sup>, Mohammad Madallh Alhabahba<sup>38</sup>, Alvaro Mailhos<sup>23</sup>, Zoi Manesi<sup>34</sup>, Rocio Martinez<sup>39</sup>, Sarah L. McKerchar<sup>14</sup>, Norbert Meskó<sup>36</sup>, Girishwar Misra<sup>40</sup>, Hoang Moc Lan<sup>41</sup>, Conal Monaghan<sup>14</sup>, Emanuel C. Mora<sup>42</sup>, Alba Moya-Garofano<sup>39</sup>, Bojan Musil<sup>27</sup>, Jean C. Natividade<sup>43</sup>, George Nizharadze<sup>44</sup>, Elisabeth Oberzaucher<sup>45</sup>, Mohd S. Omar Fauzee<sup>46</sup>, Ernest Onyishi<sup>9</sup>, Baris Özener<sup>10</sup>, Ariela F. Pagani<sup>37</sup>, Vilmante Pakalniskiene<sup>47</sup>, Miriam Parise<sup>37</sup>, Farid Pazhoohi<sup>48</sup>, Mariia Perun<sup>49</sup>, Annette Pisanski<sup>42, 50</sup>, Nejc Plohl<sup>27</sup>, Camelia Popa<sup>21</sup>, Pavol Prokop<sup>52, 53</sup>, Muhammad Rizwan<sup>54</sup>, Mario Sainz<sup>4</sup>, Svjetlana Salkičević<sup>28</sup>, Ruta Sargautyte<sup>47</sup>, Susanne Schmehl<sup>45</sup>, Oksana Senyk<sup>49</sup>, Rizwana Shaikh<sup>55</sup>, Shivantika Sharad<sup>56</sup>, Franco Simonetti<sup>57</sup>, Meri Tadinac<sup>28</sup>, Truong Thi Khanh Ha<sup>41</sup>, Trinh Thi Linh<sup>41</sup>, Karina Ugalde González<sup>58</sup>, Nguyen Van Luot<sup>41</sup>, Christin-</b> <b>Melanie Vauclair<sup>24</sup>, Luis D. Vega Araya<sup>58</sup>, Gyesook Yoo<sup>60</sup>, Stanislava Yordanova Stoyanova<sup>26</sup>, Zainab F. Zadeh<sup>59</sup>, Maja Zupančič<sup>35</sup>.</b>
DOI: 10.6084/m9.figshare.9747851
title: Genbank accession numbers for virus sequences used in Fig.5. from Single Cell Ecogenomics reveals mating types of individual cells and ssDNA viral infections in the Smallest Photosynthetic Eukaryotes

description: Planktonic photosynthetic organisms of the class Mamiellophyceae include the smallest eukaryotes (less than 2 µm), are globally distributed and form the basis of coastal marine ecosystems. Eight complete fully annotated 13–22 Mb genomes from three genera, <i>Ostreococcus</i>, <i>Bathycoccus</i> and <i>Micromonas</i>, are available from previously isolated clonal cultured strains and provide an ideal resource to explore the scope and challenges of analysing Single Cell Amplified Genomes (SAGs) isolated from a natural environment. We assembled data from 12 SAGs sampled during the Tara Oceans expedition to gain biological insights about their <i>in situ</i> ecology, which might be lost by isolation and strain culture. Although the assembled nuclear genomes were incomplete, they were large enough to infer the mating types of four <i>Ostreococcus</i> SAGs. The systematic occurrence of sequences from the mitochondria and chloroplast, representing less than 3% of the total cell's DNA, intimates that SAGs provide suitable substrates for detection of non-target sequences, such as those of virions. Analysis of the non-Mamiellophyceae assemblies, following filtering out cross-contaminations during the sequencing process, revealed two novel 1.6 and 1.8 kb circular DNA viruses, and the presence of specific Bacterial and Oomycete sequences suggests that these organisms might co-occur with the Mamiellales.This article is part of the discussion meeting issue ‘Single cell ecology’.