DOI: 10.5061/dryad.kwh70rzf9
title: Soil microbial legacies and drought mediate diversity-invasibility relationships in non-native communities

description: High native species diversity generally suppresses non-native invasions,
 but many ecosystems are now characterized by non-native assemblages that
 vary in species diversity. How this non-native species diversity affects
 subsequent invaders and its environmental dependence remains unclear.
 Here, we conducted a plant-soil feedback experiment to investigate how
 non-native plant species diversity affects the growth of subsequent
 non-native plants, the role of soil microbes in this process, and the
 dependence of these patterns on drought. We found that under well-watered
 conditions, the biomass of subsequent invaders was higher with soil
 inocula generated by high non-native diversity, which was associated with
 higher arbuscular mycorrhizal fungal richness. However, under drought
 conditions, the biomass of subsequent invaders did not depend on soil
 inocula generated by non-native diversity. Our study reveals soil
 microbial legacies likely contribute to the commonly observed
 co-occurrence of multiple non-native species in nature and the importance
 of environmental conditions for these effects.
description: # **Soil microbial legacies and drought mediate diversity-invasibility
 relationships in non-native communities** ####
 **Appendix\_S1\_Information\_for\_non\_native\_species** Information for
 non-native species and non-native assemblage used in the two-phase
 feedback experiment. #### **Appendix\_S2\_R\_codes** R code for all the
 data analysis and all figures. #### **Data01\_soil\_AMF\_rarefied** |
 Non\_native\_species\_diversity | Non-native species diversity in phase I
 (1, 2, and 4) | | --------------------------------- |
 --------------------------------------------------------------------------------------------------------------------------------------------------------- | | Log\_diversity | Log-transformed (Non-native species diversity) | | Non\_native\_species\_assemblages | six monocultures (D1-1 to D1-6), six mixtures of two species (D2-1 to D2-6), and six mixtures of four species (D4-1 to D4-6), see Appendix\_S1 for detail | | 8d299e0078705469d02e60b50f2c32bb | ASV\_ID, number of reads for AMF ASV | #### **Data09\_soil\_pathogen\_rarefied** | Non\_native\_species\_diversity | Non-native species diversity in phase I (1, 2, and 4) | | --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------- | | Log\_diversity | Log-transformed (Non-native species diversity) | | Non\_native\_species\_assemblages | six monocultures (D1-1 to D1-6), six mixtures of two species (D2-1 to D2-6), and six mixtures of four species (D4-1 to D4-6), see Appendix\_S1 for detail | | bd7507fffdcc8874ff0dd036b21e19a9 | ASV\_ID, number of reads for ASV of soil pathogen | #### **Data02\_soil\_microbes** | Non\_native\_species\_diversity | Non-native species diversity in phase I (1, 2, and 4) | | --------------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------- | | Log\_diversity | Log-transformed (Non-native species diversity) | | Non\_native\_species\_assemblages | six monocultures (D1-1 to D1-6), six mixtures of two species (D2-1 to D2-6), six mixtures of four species (D4-1 to D4-6), see Appendix\_S1 for detail | | Soil\_sample | Soil samples in phase I (mixing soils from 2 pots of each diversity and assemblage combination) | | AMF\_richness | Richness of AMF at the end of phase I | | Pathogen\_richness | Richness of the pathogen at the end of phase I | | Pathogen\_relative\_abundance | Abundance of the pathogen at the end of phase I | | Glomeraceae\_richness | Richness of Glomeraceae at the end of phase I | #### **Data03\_individual\_species\_and\_AMF** | Non\_native\_species\_diversity | Non-native species diversity in phase I (1, 2, and 4) | | --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------- | | Log\_diversity | Log-transformed (Non-native species diversity) | | Non\_native\_species\_assemblages | six monocultures (D1-1 to D1-6), six mixtures of two species (D2-1 to D2-6), and six mixtures of four species (D4-1 to D4-6), see Appendix\_S1 for detail | | Soil\_sample | Soil samples in phase I (mixing soils from 2 pots of each diversity and assemblage combination) | | AT/AC/AA/EC/PU/SC | Non-native species present in non-native assemblages (Y) or absent (N) | | AMF\_richness | Richness of AMF at the end of phase I | | AMF\_PCoA1 | Axis 1 of PCoA of AMF community | | AMF\_PCoA2 | Axis 2 of PCoA of AMF community | #### **Data04\_subsequent\_plant\_biomass** | Non\_native\_species\_diversity | Non-native species diversity in phase I (1, 2, and 4) | | --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------- | | Log\_diversity | Log-transformed (Non-native species diversity) | | Non\_native\_species\_assemblages | six monocultures (D1-1 to D1-6), six mixtures of two species (D2-1 to D2-6), and six mixtures of four species (D4-1 to D4-6), see Appendix\_S1 for detail | | Soil\_sample | Soil samples in phase I (mixing soils from 2 pots of each diversity and assemblage combination) | | Non\_native\_species\_identity | Plant species used in phase II | | Home\_away\_soil | Soil when responding species in phase II was present in assemblages in phase I (home) or absent (away) | | Watering\_treatment | Watering treatment in phase II: well-watered or drought | | Biomass | Biomass (g) for non-native species at the end of phase II, including aboveground and belowground biomass | #### **Data05\_microbes\_and\_subsequent\_plant\_biomass** | Non\_native\_species\_diversity | Non-native species diversity in phase I (1, 2, and 4) | | --------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------- | | Log\_diversity | Log-transformed (Non-native species diversity) | | Non\_native\_species\_assemblages | six monocultures (D1-1 to D1-6), six mixtures of two species (D2-1 to D2-6), and six mixtures of four species (D4-1 to D4-6), see Appendix\_S1 for detail | | Soil\_sample | Soil samples in phase I (mixing soils from 2 pots of each diversity and assemblage combination) | | AMF\_richness | Richness of AMF at the end of phase I | | Pathogen\_richness | Richness of pathogen at the end of phase I | | Glomeraceae\_richness | Richness of Glomeraceae at the end of phase I | | Watering\_treatment | Watering treatment in phase II: well-watered or drought | | Average\_biomass | Average biomass (g) of non-native species in each soil inocula in each watering treatment | #### **Data06\_plots\_in\_field\_survey** Numbers of plots with different diversities of non-native plants in field survey. | Non\_native\_species\_diversity | Non-native species diversity in each plot in a field survey | | ------------------------------- | ---------------------------------------------------------------------------------- | | Plot\_number | Numbers of plots with different diversities of non-native plants in a field survey | #### **Data07\_otu\_fungi\_unrarefied** | #ASV ID | ID of ASVs in soil samples | | ------- | -------------------------- | | D1-1-1 | Number of soil samples | #### **Data08\_otu\_AMF\_unrarefied** | #ASV ID | ID of ASVs in soil samples | | ------- | -------------------------- | | D1-1-1 | Number of soil samples | #### **Data10\_home\_away\_statisitic\_result** | Non\_native\_species\_identity | Plant species used in phase II | | ------------------------------ | ------------------------------------------------------------------------------------------------------ | | Home\_away\_soil | Soil when responding species in phase II was present in assemblages in phase I (home) or absent (away) | | Watering\_treatment | Watering treatment in phase II: well-watered or drought | | emmean | Average biomass (g) for non-native species in the home or away soil in each watering treatment | | SE | Standard error of emmean | #### **Data11\_AMF\_composition** | AMF\_family | Taxon name of AMF at family level | | ----------- | ------------------------------------------------------------------------- | | Proportion | Relative abundance of each taxon at the family level in the AMF community |