cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25549/whit-c170-43127
title: Father Bernard Hubbard & Holmes at Carthay Circle Theatre, Los Angeles, CA, 1934

description: 4 photographs of Father Bernard Hubbard & Holmes at Carthay Circle Theatre, Los Angeles, CA, 1934. "Subject: Father Bernard Hubbard & Holmes at Carthay Circle Theatre; Client: Pacific Geographic Society, Chamber of Commerce b[ui]ld[in]g, Bert Phillips; Original Print Order: 2 ea[ch]; Size: 8x10; Finish: gl[ossy]; Job: 10-25-141; Year: 1934" -- on envelope front. "Operator's Report Name: Henry; 4 Original Photographs--Size: 8x10 int[erior]; Amount: 13.50" -- on envelope back.
DOI: 10.6084/m9.figshare.24982932
title: STING inhibition enables efficient plasmid-based gene expression in primary vascular cells: a simple and cost-effective transfection protocol

description: Plasmid transfection in cells is widely employed to express exogenous proteins, offering valuable mechanistic insight into their function(s). However, plasmid transfection efficiency in primary vascular endothelial cells (ECs) and smooth muscle cells (SMCs) is restricted with lipid-based transfection reagents such as Lipofectamine. The STING pathway, activated by foreign DNA in the cytosol, prevents foreign gene expression and induces DNA degradation. To address this, we explored the potential of STING inhibitors on the impact of plasmid expression in primary ECs and SMCs. Primary human aortic endothelial cells (HAECs) were transfected with a bicistronic plasmid expressing cytochrome b5 reductase 4 (CYB5R4) and enhanced green fluorescent protein (EGFP) using Lipofectamine 3000. Two STING inhibitors, MRT67307 and BX795, were added during transfection and overnight post-transfection. As a result, MRT67307 significantly enhanced CYB5R4 and EGFP expression, even 24 hours after its removal. In comparison, MRT67307 pretreatment did not affect transfection, suggesting the inhibitor’s effect was readily reversible. The phosphorylation of endothelial nitric oxide synthase (eNOS) at Serine 1177 (S1177) by vascular endothelial growth factor is essential for endothelial proliferation, migration, and survival. Using the same protocol, we transfected wild-type and phosphorylation-incapable mutant (S1177A) eNOS in HAECs. Both forms of eNOS localized on the plasma membrane, but only the wild-type eNOS was phosphorylated by vascular endothelial growth factor treatment, indicating normal functionality of overexpressed proteins. MRT67307 and BX795 also improved plasmid expression in human and rat aortic SMCs. In conclusion, this study presents a modification enabling efficient plasmid transfection in primary vascular ECs and SMCs, offering a favorable approach to studying protein function(s) in these cell types, with potential implications for other primary cell types that are challenging to transfect.
DOI: 10.6084/m9.figshare.24982932.v2
title: STING inhibition enables efficient plasmid-based gene expression in primary vascular cells: a simple and cost-effective transfection protocol

description: Plasmid transfection in cells is widely employed to express exogenous proteins, offering valuable mechanistic insight into their function(s). However, plasmid transfection efficiency in primary vascular endothelial cells (ECs) and smooth muscle cells (SMCs) is restricted with lipid-based transfection reagents such as Lipofectamine. The STING pathway, activated by foreign DNA in the cytosol, prevents foreign gene expression and induces DNA degradation. To address this, we explored the potential of STING inhibitors on the impact of plasmid expression in primary ECs and SMCs. Primary human aortic endothelial cells (HAECs) were transfected with a bicistronic plasmid expressing cytochrome b5 reductase 4 (CYB5R4) and enhanced green fluorescent protein (EGFP) using Lipofectamine 3000. Two STING inhibitors, MRT67307 and BX795, were added during transfection and overnight post-transfection. As a result, MRT67307 significantly enhanced CYB5R4 and EGFP expression, even 24 hours after its removal. In comparison, MRT67307 pretreatment did not affect transfection, suggesting the inhibitor’s effect was readily reversible. The phosphorylation of endothelial nitric oxide synthase (eNOS) at Serine 1177 (S1177) by vascular endothelial growth factor is essential for endothelial proliferation, migration, and survival. Using the same protocol, we transfected wild-type and phosphorylation-incapable mutant (S1177A) eNOS in HAECs. Both forms of eNOS localized on the plasma membrane, but only the wild-type eNOS was phosphorylated by vascular endothelial growth factor treatment, indicating normal functionality of overexpressed proteins. MRT67307 and BX795 also improved plasmid expression in human and rat aortic SMCs. In conclusion, this study presents a modification enabling efficient plasmid transfection in primary vascular ECs and SMCs, offering a favorable approach to studying protein function(s) in these cell types, with potential implications for other primary cell types that are challenging to transfect.
DOI: 10.6084/m9.figshare.24982932.v1
title: STING inhibition enables efficient plasmid-based gene expression in primary vascular cells: a simple and cost-effective transfection protocol

description: Plasmid transfection in cells is widely employed to express exogenous proteins, offering valuable mechanistic insight into their function(s). However, plasmid transfection efficiency in primary vascular endothelial cells (ECs) and smooth muscle cells (SMCs) is restricted with lipid-based transfection reagents such as Lipofectamine. The STING pathway, activated by foreign DNA in the cytosol, prevents foreign gene expression and induces DNA degradation. To address this, we explored the potential of STING inhibitors on the impact of plasmid expression in primary ECs and SMCs. Primary human aortic endothelial cells (HAECs) were transfected with a bicistronic plasmid expressing cytochrome b5 reductase 4 (CYB5R4) and enhanced green fluorescent protein (EGFP) using Lipofectamine 3000. Two STING inhibitors, MRT67307 and BX795, were added during transfection and overnight post-transfection. As a result, MRT67307 significantly enhanced CYB5R4 and EGFP expression, even 24 hours after its removal. In comparison, MRT67307 pretreatment did not affect transfection, suggesting the inhibitor’s effect was readily reversible. The phosphorylation of endothelial nitric oxide synthase (eNOS) at Serine 1177 (S1177) by vascular endothelial growth factor is essential for endothelial proliferation, migration, and survival. Using the same protocol, we transfected wild-type and phosphorylation-incapable mutant (S1177A) eNOS in HAECs. Both forms of eNOS localized on the plasma membrane, but only the wild-type eNOS was phosphorylated by vascular endothelial growth factor treatment, indicating normal functionality of overexpressed proteins. MRT67307 and BX795 also improved plasmid expression in human and rat aortic SMCs. In conclusion, this study presents a modification enabling efficient plasmid transfection in primary vascular ECs and SMCs, offering a favorable approach to studying protein function(s) in these cell types, with potential implications for other primary cell types that are challenging to transfect.
DOI: 10.25549/whit-c170-44001
title: New station - West 3rd Street & Serrano Avenue, Los Angeles, CA, 1933

description: 6 photographs of men and new service station, West 3rd Street & Serrano Avenue, Los Angeles, CA, 1933. "Subject: New station - 3rd & Serrano; Client: Union Oil; Original Print Order: 1 ea[ch]; Size: 8x10, 5x7; Finish: gl[ossy]; Mount: Nice stuff; Re-order: 1#4; Year: 1933; Job: 7-12-88" -- on envelope front. "Operator's Report Name: Dean; 6 Original Photographs-Size: 8x10 gl[ossy]; Amount: 18.00; Extra Charges: Ted Stromberger" -- on envelope back.
DOI: 10.25549/whit-c170-43125
title: Father Bernard Hubbard & Holmes at Carthay Circle Theatre, Los Angeles, CA, 1934

description: 4 photographs of Father Bernard Hubbard & Holmes at Carthay Circle Theatre, Los Angeles, CA, 1934. "Subject: Father Bernard Hubbard & Holmes at Carthay Circle Theatre; Client: Pacific Geographic Society, Chamber of Commerce b[ui]ld[in]g, Bert Phillips; Original Print Order: 2 ea[ch]; Size: 8x10; Finish: gl[ossy]; Job: 10-25-141; Year: 1934" -- on envelope front. "Operator's Report Name: Henry; 4 Original Photographs--Size: 8x10 int[erior]; Amount: 13.50" -- on envelope back.
DOI: 10.25549/whit-c170-109560
title: Wrestling at Olympic Arena, Angel vs Dick Rutz, Los Angeles, Calif., 1940.

description: 3 photographs of 2 wrestlers and a referee in a boxing ring with an audience, taken from a top tier of the Grand Olympic Auditorium in Los Angeles, California, 1940 July 13. "Angel vs Dick Rutz" -- on envelope.
DOI: 10.25549/whit-c170-43702
title: Vis-O-Matic slides, reel 4, Southern California, 1935

description: 17 photographs of men and service station, Southern California, 1935. "Subejct: Visomatic Slides, Reel 4, See also I. 8x10; Client: Standard Oil Co; Year: 1935; Job: 4-12-105-H" -- on envelope.
DOI: 10.25549/whit-c170-109619
title: Midget race cars, California, 1940

description: 10 photographs of "midget race cars" that vary between about 14 inches to 2 feet long, including the oval race track, audience, car owners, a shop where the cars are fixed, and a scale where they are weighed, California, 1940 July 16. Three of the partitions in the workshop have name labels: Herb Shapiro, Al Burns, and Bill Dempsey. The car being weighed (no. 43) tipped the scale at 9 lb.
DOI: 10.25549/whit-c170-43727
title: Hane Brothers service station, Southern California, 1934

description: 3 photographs of Hane Brothers service station, Southern California, 1934. "Subject: Hane Bro[ther]s Service Station, to show gas prices; Client: Shell Oil Company, Hitchman; Original Print Order: 2 ea[ch]; Size: 8x10; Finish: gl[ossy]; Job: 1-15-70; Year: 1934" -- on envelope front. "Operator's Report Name: Joe + William; 2 Original Photographs-Size: 8x10; Amount: 15.00; Extra Charges: Hitchman" -- on envelope back.
DOI: 10.25549/whit-c170-43734
title: Earl Cooper measuring diameter of cylinder, Southern California, 1934

description: 3 photographs of Earl Cooper measuring diameter of cylinder, Southern California, 1934. "Subject: Earl Cooper measuring diameter of cylinder; Client: Union Oil Co[mpany]; Original Print Order: 1 ea[ch], 1; Size: 8x10, 5x7; Finish: gl[ossy]; Mount: full neg[ative], for files; Job: 12-19-105; Year: 1934" -- on envelope front. "Operator's Report Name: Dick; 3 Original Photographs-Size: 8x10; Amount: 7.80; Extra Charges: Stromheyer" -- on envelope back.
DOI: 10.25549/whit-c170-107424
title: La Jolla, Casa Mañana, La Jolla, Calif., 1939

description: 12 photographs of models at Casa de Mañana and the beach, La Jolla, California, 1939 September. A man and woman sit poolside and at the bar, play on the beach, take tea, play golf, and dine.
DOI: 10.15468/dl.5rhg25
title: Occurrence Download

description: A dataset containing 851 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-100.512 21.505,-100.438 21.505,-100.438 21.567,-100.512 21.567,-100.512 21.505))",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 851 records from 23 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0176063-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.pglhhh
title: Occurrence Download

description: A dataset containing 473 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Santolina L.",
 "Country is Portugal"
 ]
}

 The dataset includes 473 records from 22 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0101332-160910150852091/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ccki7b
title: Occurrence Download

description: A dataset containing 58024 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Plantae",
 "Country is Madagascar",
 "Geometry POLYGON((43.769531 -20.591652,44.428710 -20.715015,44.780273 -19.766703,44.560546 -18.729501,44.516601 -17.392579,44.956054 -16.678293,46.538085 -15.919073,48.383789 -14.859850,48.955078 -13.539200,49.262695 -12.897489,49.877929 -12.468760,49.262695 -11.910353,48.471679 -11.867350,43.901367 -15.580710,43.286132 -19.518375,43.769531 -20.591652))"
 ]
}

 The dataset includes 58024 records from 45 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0101333-160910150852091/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.53vaky
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Orthriophis taeniurus (Cope, 1861))",
 "BasisOfRecord is one of (Machine Observation, Human Observation, Observation, Literature Occurrence)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 14 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014097-250310093411724/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.y9ax5d
title: Occurrence Download

description: A dataset containing 594 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Marmota marmota marmota"
 ]
}

 The dataset includes 594 records from 22 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014098-250310093411724/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rnjy74
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Tetrancistrum suezicum (Paperna, 1972) Kritsky, Galli & Yang, 2007)",
 "BasisOfRecord is one of (Machine Observation, Human Observation, Observation, Literature Occurrence)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014099-250310093411724/datasets/export for details.
DOI: 10.15468/dl.h8yz32
title: Occurrence Download

description: A dataset containing 1421 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Fagopyrum esculentum Moench"
 ]
}

 The dataset includes 1421 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014100-250310093411724/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c7qfa6
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Genaparlatoria pseudaspidiotus MacGillivray, 1921",
 "HasGeospatialIssue is false",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014102-250310093411724/datasets/export for details.
DOI: 10.15468/dl.6bxrm7
title: Occurrence Download

description: A dataset containing 33512412 species occurrences available in GBIF matching the query:
{
 "Country" : [
 "is Colombia"
 ]
}

 The dataset includes 33512412 records from 4708 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014083-250310093411724/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yhcn3q
title: Occurrence Download

description: A dataset containing 14977 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Occurrence evidence)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Prunus armeniaca L."
 ]
}

 The dataset includes 14977 records from 145 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028264-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rwx9tx
title: Occurrence Download

description: A dataset containing 158883 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Romania",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Insecta"
 ]
}

 The dataset includes 158883 records from 121 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028242-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c2jpa7
title: Occurrence Download

description: A dataset containing 1097 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Aedeomyia squamipennis (Lynch Arribálzaga, 1878)"
 ]
}

 The dataset includes 1097 records from 28 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028268-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z96s84
title: Occurrence Download

description: A dataset containing 5684 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Occurrence evidence)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Smilax china L."
 ]
}

 The dataset includes 5684 records from 38 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028270-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.sr8kb8
title: Occurrence Download

description: A dataset containing 58744 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Occurrence evidence)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Ricinus communis L."
 ]
}

 The dataset includes 58744 records from 281 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028267-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.xhf5fn
title: Occurrence Download

description: A dataset containing 482 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Occurrence evidence)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Citrus ×aurantiifolia (Christm.) Swingle"
 ]
}

 The dataset includes 482 records from 49 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028271-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4gc7fs
title: Occurrence Download

description: A dataset containing 669 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Occurrence evidence)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Vachellia leucophloea (Roxb.) Maslin, Seigler & Ebinger"
 ]
}

 The dataset includes 669 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028272-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.neuzwa
title: Occurrence Download

description: A dataset containing 59 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Gentianella crispata (Vis.) Holub, Gentianella crispata f. candida (Beck) Jovan.-Dunj., Gentiana crispata Vis., Gentiana crispata subsp. autumnalis Pevalek, Gentiana crispata subsp. poljensis H.Ritter, Gentiana crispata subsp. visianii Pevalek, Gentiana amblyphylla Borbás, Gentianella crispata var. autumnalis (Pevalek) Bjelcic & E.Mayer, Gentianella crispata subsp. autumnalis (Pevalek) Holub, Gentianella crispata subsp. poljensis (H.Ritter) Holub, Gentianella crispata subsp. visianii (Pevalek) Holub, Gentiana crispata subsp. amblyphylla (Borbás) Pevalek, Gentianella crispata var. amblyphylla (Borbás) Bjelcic & E.Mayer, Gentiana crispata subsp. amblyphylla (Borbás) Holub, Gentianella crispata subsp. amblyphylla (Borbás) Holub)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 2000"
 ]
}

 The dataset includes 59 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028273-241126133413365/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.60952/3hff-s058
title: Przewlekły, zanikowy stan zapalny błony śluzowej żołądka z dysplazją dużego stopnia

description: Materiał zdjęciowy powstał w wyniku badania mającego na celu: analizę obrazów endoskopowych żołądka;                              analizę obrazów morfologicznych (histopatologicznych) wycinków błony śluzowej żołądka pobranych  czasie endoskopii  u pacjentów zgłaszających się z powodu dolegliwości dyspeptycznych (w analizie uwzględniono płeć i wiek badanych); ustalenie wpływu w/w czynników na częstotliwość poszczególnych jednostek chorobowych; analizę współwystępowania zakażenia Helicobakter pylori w stanach zapalnych błony śluzowej żołądka. Materiał do badania mikroskopowego stanowiły wycinki błony śluzowej żołądka pobrane w trakcie badania gastroskopowego od 391chorych kobiet i 639 chorych mężczyzn, którzy zgłaszali się do Poradni Gastrologicznych z powodu różnych dolegliwości dyspeptycznych trwających ok. 2,5 lat. Od każdego chorego pobierano rutynowo po dwa wycinki z okolicy podwpustowej, z okolicy trzonu żołądka i z jamy odźwiernikowej (antrum pylori). Analizę przeprowadzano z uwzględnieniem płci i grup wieku (co 10 lat, w zakresie od 10-20 do ponad 80 lat). Preparaty histopatologiczne barwiono metodami rutynowymi H i E. W wycinkach pobranych od 100 chorych wykonano barwienia pozwalające na wykrycie Helicobacter pylori metodami: zmodyfikowana metoda Giemzy, metoda "pół Grama", oraz metodą Warthina-Starry'ego pozwalająca na wykrycie pojedynczych bakterii. Badania endoskopowe wykonano gastrofiberoskopem Olympus GIF D2 i GIF K. Na zdjęciu przewlekły, zanikowy stan zapalny błony śluzowej żołądka z dysplazją dużego stopnia. Wycinek pobrany z części przedodźwiernikowej żołądka. W obrębie cewy gruczołowej widać kilka warstw komórek, pleomorfizm, hyperchromazję jąder komórkowych i przesunięcie wskaźnika jądrowo -cytoplazmatycznego na korzyść jądra. Utrata wydzielania śluzu. H. E. x 400.
. Dokumentacja zdjęciowa preparatów histopatologicznych powstała przy użyciu mikroskopu świetlnego. Opracowanie techniczne na kliszy czarno-białej przez pracownię fotograficzną Instytutu Biostruktury Akademii Medycznej w Warszawie.