cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22308666
title: Genetic diversity of the species Cnesterodon hypselurus (Cyprinodontiformes: Poeciliidae) in Cinzas River basin: new record and headwater capture evidences

description: Abstract Cnesterodon hypselurus is a small fish that has a restricted distribution in southern Brazil, including headwaters of the Tibagi and Itararé river basins (Upper Paraná River). This study reported C. hypselurus in a headwater of Cinzas River basin, where there were no previous records of this species, and employed microsatellite loci and mitochondrial haplotypes in a population genetic analysis. A total of 57 specimens was analyzed, including 30 from Cinzas River basin, 25 from Itararé River basin and two from Tibagi River basin. Results indicated low genetic diversity levels (HE = 0.334 and h = 0.246) for the sample from Cinzas River, suggesting reflections of a founder effect after the species had dispersed from one watershed to another, possibly by headwater captures. Since different populations were detected between the Cinzas and Itararé rivers (DEST = 0.248, P-value &lt; 0.05) and other occurrence sites are still unknown in the Cinzas River basin, the data herein have great relevance and should be taken into account in future management and conservation actions, as well as in evolutionary studies of C. hypselurus.
DOI: 10.6084/m9.figshare.22308666.v1
title: Genetic diversity of the species Cnesterodon hypselurus (Cyprinodontiformes: Poeciliidae) in Cinzas River basin: new record and headwater capture evidences

description: Abstract Cnesterodon hypselurus is a small fish that has a restricted distribution in southern Brazil, including headwaters of the Tibagi and Itararé river basins (Upper Paraná River). This study reported C. hypselurus in a headwater of Cinzas River basin, where there were no previous records of this species, and employed microsatellite loci and mitochondrial haplotypes in a population genetic analysis. A total of 57 specimens was analyzed, including 30 from Cinzas River basin, 25 from Itararé River basin and two from Tibagi River basin. Results indicated low genetic diversity levels (HE = 0.334 and h = 0.246) for the sample from Cinzas River, suggesting reflections of a founder effect after the species had dispersed from one watershed to another, possibly by headwater captures. Since different populations were detected between the Cinzas and Itararé rivers (DEST = 0.248, P-value &lt; 0.05) and other occurrence sites are still unknown in the Cinzas River basin, the data herein have great relevance and should be taken into account in future management and conservation actions, as well as in evolutionary studies of C. hypselurus.
DOI: 10.6084/m9.figshare.22308667
title: Microtomographic, histomorphometric, and molecular features show a normal alveolar bone healing process in iNOS-deficient mice along a compensatory upregulation of eNOS and nNOS isoforms

description: Abstract Inducible nitric oxide synthase (iNOS) is one of the enzymes responsible for the synthesis of nitric oxide (NO), which is an important signaling molecule with effects on blood vessels, leukocytes, and bone cells. However, the role of iNOS in alveolar bone healing remains unclear. This study investigated the role of iNOS in alveolar bone healing after tooth extraction in mice. Methodology C57Bl/6 wild type (WT) and iNOS genetically deficient (iNOS-KO) mice were subjected to upper incision tooth extraction, and alveolar bone healing was evaluated by micro-computed tomography (μCT) and histological/histomorphometric, birefringence, and molecular methods. Results The expression of iNOS had very low control conditions, whereas a significant increase is observed in healing sites of WT mice, where iNOS mRNA levels peak at 7d time point, followed by a relative decrease at 14d and 21d. Regarding bone healing, both WT and iNOS-KO groups showed the usual phases characterized by the presence of clots, granulation tissue development along the inflammatory cell infiltration, angiogenesis, proliferation of fibroblasts and extracellular matrix synthesis, bone neoformation, and remodeling. The overall micro-computed tomography and histomorphometric and birefringence analyses showed similar bone healing readouts when WT and iNOS-KO strains are compared. Likewise, Real-Time PCR array analysis shows an overall similar gene expression pattern (including bone formation, bone resorption, and inflammatory and immunological markers) in healing sites of WT and iNOS-KO mice. Moreover, molecular analysis shows that nNOS and eNOS were significantly upregulated in the iNOS-KO group, suggesting that other NOS isoforms could compensate the absence of iNOS. Conclusion The absence of iNOS does not result in a significant modulation of bone healing readouts in iNOS-KO mice. The upregulation of nNOS and eNOS may compensate iNOS absence, explaining the similar bone healing outcome in WT and iNOS-KO strains.
DOI: 10.6084/m9.figshare.22308667.v1
title: Microtomographic, histomorphometric, and molecular features show a normal alveolar bone healing process in iNOS-deficient mice along a compensatory upregulation of eNOS and nNOS isoforms

description: Abstract Inducible nitric oxide synthase (iNOS) is one of the enzymes responsible for the synthesis of nitric oxide (NO), which is an important signaling molecule with effects on blood vessels, leukocytes, and bone cells. However, the role of iNOS in alveolar bone healing remains unclear. This study investigated the role of iNOS in alveolar bone healing after tooth extraction in mice. Methodology C57Bl/6 wild type (WT) and iNOS genetically deficient (iNOS-KO) mice were subjected to upper incision tooth extraction, and alveolar bone healing was evaluated by micro-computed tomography (μCT) and histological/histomorphometric, birefringence, and molecular methods. Results The expression of iNOS had very low control conditions, whereas a significant increase is observed in healing sites of WT mice, where iNOS mRNA levels peak at 7d time point, followed by a relative decrease at 14d and 21d. Regarding bone healing, both WT and iNOS-KO groups showed the usual phases characterized by the presence of clots, granulation tissue development along the inflammatory cell infiltration, angiogenesis, proliferation of fibroblasts and extracellular matrix synthesis, bone neoformation, and remodeling. The overall micro-computed tomography and histomorphometric and birefringence analyses showed similar bone healing readouts when WT and iNOS-KO strains are compared. Likewise, Real-Time PCR array analysis shows an overall similar gene expression pattern (including bone formation, bone resorption, and inflammatory and immunological markers) in healing sites of WT and iNOS-KO mice. Moreover, molecular analysis shows that nNOS and eNOS were significantly upregulated in the iNOS-KO group, suggesting that other NOS isoforms could compensate the absence of iNOS. Conclusion The absence of iNOS does not result in a significant modulation of bone healing readouts in iNOS-KO mice. The upregulation of nNOS and eNOS may compensate iNOS absence, explaining the similar bone healing outcome in WT and iNOS-KO strains.
DOI: 10.6084/m9.figshare.22308669.v1
title: REGULATION BY RESULTS AND RECONFIGURATIONS IN INSTITUTIONAL ARRANGEMENTS AIMED AT THE DEMOCRATIC SCHOOL GOVERNMENT

description: ABSTRACT: In the context of the growing implementation of accountability measures in basic education identified with policies of regulation by results, this paper analyzes possible reconfigurations of institutional arrangements to account for democratic school government in terms of participation and social control. We conducted this documental research in two Brazilian capitals (Fortaleza [CE] and Palmas [TO]). We surveyed, examined, and systematized target documents, guidelines, measures, and conditions for the democratic government of public basic education, which were in line with result-based regulation policies carried out since the 2000s. Nonetheless, we conclude that the ensemble of changes in each capital may indicate some favoring of the institutional conditions of participation and social control. However, we highlight that the suggested potential of these changes seems to lose power when faced with a political-institutional framework that emphasizes measures identified with policies of regulation by results.
DOI: 10.6084/m9.figshare.22308669
title: REGULATION BY RESULTS AND RECONFIGURATIONS IN INSTITUTIONAL ARRANGEMENTS AIMED AT THE DEMOCRATIC SCHOOL GOVERNMENT

description: ABSTRACT: In the context of the growing implementation of accountability measures in basic education identified with policies of regulation by results, this paper analyzes possible reconfigurations of institutional arrangements to account for democratic school government in terms of participation and social control. We conducted this documental research in two Brazilian capitals (Fortaleza [CE] and Palmas [TO]). We surveyed, examined, and systematized target documents, guidelines, measures, and conditions for the democratic government of public basic education, which were in line with result-based regulation policies carried out since the 2000s. Nonetheless, we conclude that the ensemble of changes in each capital may indicate some favoring of the institutional conditions of participation and social control. However, we highlight that the suggested potential of these changes seems to lose power when faced with a political-institutional framework that emphasizes measures identified with policies of regulation by results.
DOI: 10.6084/m9.figshare.22308670
title: Nutrient compositional variation and interrelationship in the muscle of wild and plastic-reared brachyuran

description: ABSTRACT. Edible brachyurans are recognized as a popular source of food due to their delicious taste and nutritional quality. This study investigated the nutrient compositional variation and interrelationship in the muscle of wild and plastic-reared Cardisoma armatum. The plastic-reared crab had 27.81 ± 2.29 g 100 g-1 protein while crab from the wild contained 22.45 ± 2.65 g 100 g-1 protein. The difference in protein content of plastic-reared and its counterpart from the mangrove swamp was not significantly different (p &gt; 0.05). This is also true of other proximate composition except that wild crabs were slightly higher in both crude fat (2.68 ± 0.35 g 100 g-1) and carbohydrate (5.89 ± 3.05 g 100 g-1). Generally, the total energy contributed due to protein, carbohydrate and fat in the tissues of both wild and plastic-reared C. armatum are similar. In the wild crab, contents of calcium (16083.27 ± 2127.90 mg 100 g-1) and phosphorus (1191.42 ± 199.21) were relatively higher, while contents of magnesium (368.69 ± 111.05 mg 100 g-1), sodium (125.30 ± 11.18 mg 100 g-1) and potassium (87.36 ± 7.27 mg 100 g-1) were relatively higher in plastic-reared crabs. All significant mineral ratios in wild and plastic-reared C. amartum fall within acceptable range. The positive relationship in the nutritional quality indicates that changes in proximate composition are associated with changes in mineral contents of the crab tissue.
DOI: 10.6084/m9.figshare.22308670.v1
title: Nutrient compositional variation and interrelationship in the muscle of wild and plastic-reared brachyuran

description: ABSTRACT. Edible brachyurans are recognized as a popular source of food due to their delicious taste and nutritional quality. This study investigated the nutrient compositional variation and interrelationship in the muscle of wild and plastic-reared Cardisoma armatum. The plastic-reared crab had 27.81 ± 2.29 g 100 g-1 protein while crab from the wild contained 22.45 ± 2.65 g 100 g-1 protein. The difference in protein content of plastic-reared and its counterpart from the mangrove swamp was not significantly different (p &gt; 0.05). This is also true of other proximate composition except that wild crabs were slightly higher in both crude fat (2.68 ± 0.35 g 100 g-1) and carbohydrate (5.89 ± 3.05 g 100 g-1). Generally, the total energy contributed due to protein, carbohydrate and fat in the tissues of both wild and plastic-reared C. armatum are similar. In the wild crab, contents of calcium (16083.27 ± 2127.90 mg 100 g-1) and phosphorus (1191.42 ± 199.21) were relatively higher, while contents of magnesium (368.69 ± 111.05 mg 100 g-1), sodium (125.30 ± 11.18 mg 100 g-1) and potassium (87.36 ± 7.27 mg 100 g-1) were relatively higher in plastic-reared crabs. All significant mineral ratios in wild and plastic-reared C. amartum fall within acceptable range. The positive relationship in the nutritional quality indicates that changes in proximate composition are associated with changes in mineral contents of the crab tissue.
DOI: 10.6084/m9.figshare.22268666.v1
title: Influence of color and brightness on ontogenetic shelter preference by the prawn Macrobrachium rosenbergii (Decapoda: Palaemonidae)

description: ABSTRACT The giant river prawn, Macrobrachium rosenbergii (de Man, 1879), is a species of great commercial importance. It is farmed under different conditions that translate to a great range of light environments, which impact their behavior and productivity. We present the first study employing both visual modeling and beha vioral data to evaluate the ontogenetic changes in color preferences of juveniles and adults of M. rosenbergii. We offered ten shelters of different colors to juveniles and adults and registered their preferences. Our results show that shelter preference changed with ontogeny: juveniles chose shelters based on chromaticity (preference for blue), while adults based their decisions on brightness (preference for dark grey). This preference adults show for dark colors is probably associated with light avoidance behavior. We recommend providing blue shelters for juveniles and dark shelters for adults.
DOI: 10.6084/m9.figshare.22268666
title: Influence of color and brightness on ontogenetic shelter preference by the prawn Macrobrachium rosenbergii (Decapoda: Palaemonidae)

description: ABSTRACT The giant river prawn, Macrobrachium rosenbergii (de Man, 1879), is a species of great commercial importance. It is farmed under different conditions that translate to a great range of light environments, which impact their behavior and productivity. We present the first study employing both visual modeling and beha vioral data to evaluate the ontogenetic changes in color preferences of juveniles and adults of M. rosenbergii. We offered ten shelters of different colors to juveniles and adults and registered their preferences. Our results show that shelter preference changed with ontogeny: juveniles chose shelters based on chromaticity (preference for blue), while adults based their decisions on brightness (preference for dark grey). This preference adults show for dark colors is probably associated with light avoidance behavior. We recommend providing blue shelters for juveniles and dark shelters for adults.
DOI: 10.6084/m9.figshare.22308672
title: Dom Quixote, Sancho Pança e Dulcineia

description: ABSTRACT The central purpose of this article is to examine the relationship between the three characters: the knight, the squire and the lady, focusing on Don Quixote. We thereby consider a few episodes to try to understand some of the reasons that make today’s readers (and probably readers from all times) admire and intensely respect a character who, in essence, is a complete madman.
DOI: 10.6084/m9.figshare.22308672.v1
title: Dom Quixote, Sancho Pança e Dulcineia

description: ABSTRACT The central purpose of this article is to examine the relationship between the three characters: the knight, the squire and the lady, focusing on Don Quixote. We thereby consider a few episodes to try to understand some of the reasons that make today’s readers (and probably readers from all times) admire and intensely respect a character who, in essence, is a complete madman.
DOI: 10.6084/m9.figshare.21971413
title: Chemical treatment and storage of sorghum seeds produced in different management zones

description: Abstract: Precision Agriculture considers soil attributes and production aspects to define management zones. Although there is little information, it is likely that seeds produced in different management zones have different quality after chemical treatment and storage. The aim was to evaluate the physiological quality of sorghum seeds produced in different management zones, after chemical treatment and storage. The management zones were defined from interpolated maps of soil and vegetation attributes with the aid of the Smart Map - SMP plugin, in a Qgis environment. After harvest, the seeds were treated with Thiamethoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxyl-M and a control treatment (water). Then, they were stored for 0, 60 and 120 days. The seeds were evaluated for their quality through the following tests: moisture, germination, dry mass of seedlings, electrical conductivity, and accelerated aging. The design was completely randomized in a 4 × 3 factorial scheme, with the management zones being analyzed independently. Sorghum seed treatments with insecticides cause greater phytotoxicity during storage in both management zones, unlike the fungicide treatment. Sorghum seeds produced in high-management zones have high physiological quality and less deterioration during storage. Sorghum seeds produced in low-management zones treated with insecticides, as the storage time increases, show lower vigor due to deterioration and phytotoxicity.
DOI: 10.6084/m9.figshare.21971413.v1
title: Chemical treatment and storage of sorghum seeds produced in different management zones

description: Abstract: Precision Agriculture considers soil attributes and production aspects to define management zones. Although there is little information, it is likely that seeds produced in different management zones have different quality after chemical treatment and storage. The aim was to evaluate the physiological quality of sorghum seeds produced in different management zones, after chemical treatment and storage. The management zones were defined from interpolated maps of soil and vegetation attributes with the aid of the Smart Map - SMP plugin, in a Qgis environment. After harvest, the seeds were treated with Thiamethoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxyl-M and a control treatment (water). Then, they were stored for 0, 60 and 120 days. The seeds were evaluated for their quality through the following tests: moisture, germination, dry mass of seedlings, electrical conductivity, and accelerated aging. The design was completely randomized in a 4 × 3 factorial scheme, with the management zones being analyzed independently. Sorghum seed treatments with insecticides cause greater phytotoxicity during storage in both management zones, unlike the fungicide treatment. Sorghum seeds produced in high-management zones have high physiological quality and less deterioration during storage. Sorghum seeds produced in low-management zones treated with insecticides, as the storage time increases, show lower vigor due to deterioration and phytotoxicity.
DOI: 10.6084/m9.figshare.22308676.v1
title: ‘Leather on leather’: men with homosexual practices and HIV prevention in the Recife Metropolitan Region

description: ABSTRACT In this text we address sexual practices and HIV prevention in the circuits of men who have sex with men (MSM) in the Metropolitan Region of Recife (RMR), based on a behavioral survey with 380 MSM, aged between 18 and 51 years, and interviews with 20 of the respondents. The analyzed data were collected between January 2016 and February 2017, when Pre-Exposure Prophylaxis (PrEP) was not yet available and Post-Exposure Prophylaxis (PEP) was little known (51%) and used (1.3%). Unprotected Anal Sex (UAS) (50.6% in steady partners, 30.2% in casual partners) generally occurred between partners who were presumed to be HIV-negative. Serologies were inferred by ties (stranger, acquaintance, friend, boyfriend). Emotions (fear, lust, love, trust, disgust, neediness) were important in UAS configuration, almost always linked to attachments. We observed dissident pleasure regimes of heteronormativity: mouth-anus and mouth-penis; threesome and group sex. Considering the strong presence of UAS and the high prevalence of HIV in Recife (21.5%), we found the need for educational actions that introduce combined prevention techniques (condoms, PrEP, PEP, serochoice, negotiated safety etc.) through narratives that incorporate dissident bonds, emotions, and pleasure regimes, so that, when approaching the contexts of use, they enable safer choices.
DOI: 10.6084/m9.figshare.22308676
title: ‘Leather on leather’: men with homosexual practices and HIV prevention in the Recife Metropolitan Region

description: ABSTRACT In this text we address sexual practices and HIV prevention in the circuits of men who have sex with men (MSM) in the Metropolitan Region of Recife (RMR), based on a behavioral survey with 380 MSM, aged between 18 and 51 years, and interviews with 20 of the respondents. The analyzed data were collected between January 2016 and February 2017, when Pre-Exposure Prophylaxis (PrEP) was not yet available and Post-Exposure Prophylaxis (PEP) was little known (51%) and used (1.3%). Unprotected Anal Sex (UAS) (50.6% in steady partners, 30.2% in casual partners) generally occurred between partners who were presumed to be HIV-negative. Serologies were inferred by ties (stranger, acquaintance, friend, boyfriend). Emotions (fear, lust, love, trust, disgust, neediness) were important in UAS configuration, almost always linked to attachments. We observed dissident pleasure regimes of heteronormativity: mouth-anus and mouth-penis; threesome and group sex. Considering the strong presence of UAS and the high prevalence of HIV in Recife (21.5%), we found the need for educational actions that introduce combined prevention techniques (condoms, PrEP, PEP, serochoice, negotiated safety etc.) through narratives that incorporate dissident bonds, emotions, and pleasure regimes, so that, when approaching the contexts of use, they enable safer choices.
DOI: 10.6084/m9.figshare.22308678
title: Immunogenicity profile after COVID-19 vaccination in patients with onco-hematological diseases

description: ABSTRACT Objective To evaluate the influence of onco-hematological pathologies on seroconversion to COVID-19 vaccines, in addition to the effects of chemotherapy treatment on this response. Methods The present study evaluated the immunogenic response of 76 patients with onco-hematological diseases to multiple vaccine platforms compared to 25 control individuals. Results Our results showed positive response rates of 74.02% in patients with onco-hematological diseases and 100% in controls. When analyzed according to etiological group, patients with lymphoproliferative disorders achieved a positive vaccine response rate of 58.7%, whereas those with myeloproliferative diseases achieved a 100% response rate. We also observed that patients previously exposed to COVID-19 presented a 75% increase in their antibody values after vaccination, and these values were 37% higher than those of patients who did not have such exposure. We found that patients who underwent B-lymphocyte-depleting therapy in the last 2 years before vaccination had a worse response rate of 18.75%. Conclusion Despite the immunosuppression of patients with onco-hematological diseases, caused by the biology of their diseases and treatment, benefit and safety in vaccinating these patients are observed, in view of the important recall immune response and incidence of adverse effects similar to those of the healthy population.