DOI: 10.22002/8jzxp-va985
title: Caulobacter crescentus

description: CB15N strain 1208 CreS deletion. 3 of 5, CreS deletion + A22. extended constricted area of mid-division cell; S-layer topology interesting; cytoplasmic filaments; amorphous blob; dense granulefilament segmented by Sarah
description: <strong>Tilt Series Date:</strong> 2006-04-06</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep: Ariane Briegel; data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> standard PYE media (Shapiro, Brun, etc. recipe);  cultured in 50ml flask; exponential phase; A22 added to depolymerize MreB</p>
<strong>Sample Preparation:</strong> box 519, grid 1; see Ariane Briegel's notes</p>
  SAVE_cytfil_xy1.jpg: file associated to 3D Image #295 SAVE_cytfil_xy2.jpg: file associated to 3D Image #295 SAVE_cytfil_xy_1.jpg: file associated to 3D Image #295 SAVE_cytfil_yz1.jpg: file associated to 3D Image #295 SAVE_cytfil_yz2.jpg: file associated to 3D Image #295 SAVE_cytfil_yz_1.jpg: file associated to 3D Image #295 pdd2006-04-06-3_slicer4299.jpg: Nested vesicle pdd2006-04-06-3_slicer4300.jpg: S-layer pdd2006-04-06-3_slicer4301.jpg: Granule pdd2006-04-06-3_slicer4302.jpg: Peptidoglycan pdd2006-04-06-3_slicer4303.jpg: Unknown pdd2006-04-06-3_slicer4304.jpg: Nested vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-04-06-3/rawdata/</p>cc1208_06Apr06e150t03.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.5 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        06Apr06t03bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
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        pd2006-04-06-3_clip01.rec,  Reconstruction (Pixel Size 2.4 nm) cropped by Sarah, 183.5 MB  
        
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        SAVE_cytfil_xy1.tif,  file associated to 3D Image #295, 6.1 MB  
        
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        SAVE_cytfil_xy2.tif,  file associated to 3D Image #295, 5.9 MB  
        
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        SAVE_cytfil_xy_1.tif,  file associated to 3D Image #295, 5.6 MB  
        
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        SAVE_cytfil_yz1.tif,  file associated to 3D Image #295, 6.1 MB  
        
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        SAVE_cytfil_yz2.tif,  file associated to 3D Image #295, 5.9 MB  
        
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        SAVE_cytfil_yz_1.tif,  file associated to 3D Image #295, 1.1 MB  
        
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        pd2006-04-06-3_clip01-label-Filament01.am,  filament segmented by Sarah, 1.5 MB  
        
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