DOI: 10.5061/dryad.bnzs7h4kz
title: Data from: Gating-spring stiffness increases outer-hair-cell bundle stiffness, damping, and receptor current

description: In our ears, outer-hair-cell bundles (OHBs) convert sound-induced forces
 into receptor currents that drive cochlear amplification, the process
 responsible for the micropascal-scale threshold and million-fold dynamic
 range of hearing. OHBs rely on gating springs to open
 mechanoelectrical-transduction (MET) ion channels, through which the
 receptor current flows. OHBs have larger gating-spring stiffnesses than
 other types of hair bundles, but we have a poor understanding of how
 gating-spring stiffness contributes to OHB mechanics and receptor-current
 regulation. Using experimentally-constrained mathematical models of the
 OHB, we show that the increased gating-spring stiffness in an OHB
 increases its stiffness and damping. The OHB's 3D morphology reduces
 the contribution of gating-spring stiffness to OHB stiffness, reduces the
 contribution of MET-channel gating to OHB stiffness and damping, but
 causes additional OHB damping that rises with gating-spring stiffness.
 Gating-spring stiffness increases the OHB's receptor current but
 decreases its displacement-current dynamic range. Strikingly, the
 OHB's 3D morphology causes its force-current dynamic range to
 decrease with gating-spring stiffness. Our results suggest a trade-off
 between threshold and dynamic range regulated by OHB gating-spring
 stiffness. The mathematical modeling code supporting the results is
 provided here. Three types of mathematical models are included, a 3D
 wild-type OHB model, 3D OHB models with different gating-spring
 stiffnesses, and identical-columns OHB models with different gating-spring
 stiffnesses. The code generated three data files.
description: Mathematical modeling code was written in Mathematica 13.3 and
 the data from the code was saved in mx files. The data from the mx files
 was exported to CSV files, which can be read using free
 software.
description: # Gating-Spring Stiffness Increases Outer-Hair-Cell Bundle Stiffness,
 Damping, and Receptor Current
 [https://doi.org/10.5061/dryad.bnzs7h4kz](https://doi.org/10.5061/dryad.bnzs7h4kz) ## Description of the data and file structure Simulation data was created using mathematical models. The data is available in the mx files and is in Wolfram Language serialized package format. This data can be read-in by the corresponding code files nb, which can be opened using Mathematical 13.3 or later. The data in the mx files is also available in csv files, which can be opened as a text files. The nb files can also be opened using the freely available Wolfram Player. ### Files and variables Three types of mathematical models of outer-hair-cell bundles are included. 3DOHB.nb contains the code for the wild-type outer-hair-cell bundle 3D model. 3DOHB_012624.mx contains the data for the wild-type outer-hair-cell bundle 3D model. 3DOHB_012624.CSV contains the data for the wild-type outer-hair-cell bundle 3D model. mut3DOHB.nb contains the code for 3D outer-hair-cell bundle models with different gating-spring stiffnesses. mut3DOHB_062724.mx contains the data for 3D outer-hair-cell bundle models with different gating-spring stiffnesses. mut3DOHB_062724.CSV contains the data for 3D outer-hair-cell bundle models with different gating-spring stiffnesses. mutIdenticalColumnsOHB.nb contains the code for identical-columns outer-hair-cell bundle models with different gating-spring stiffnesses. mutIdenticalColumnsOHB_071224.mx contains the data for identical-columns outer-hair-cell bundle models with different gating-spring stiffnesses. mutIdenticalColumnsOHB_071224.CSV contains the data for identical-columns outer-hair-cell bundle models with different gating-spring stiffnesses. ## Code/software Mathematica 13.3 or later, or the free Wolfram Player will open the nb files. The data files are incorporated at the top of the nb code files.