DOI: 10.22002/xm4tq-nh271
title: Caulobacter crescentus

description: Yves Brun strain 2837: CB15-del-hfsB. 1 of 2, holdfast mutants from Yves Brun, had to convert data type with Priism. 'stalked cell; storage granules; short comet-tail filaments at non-stalk end; small bits of material shaped like crumpled sheets all over; long thin filament along cell wall concave side
description: <strong>Tilt Series Date:</strong> 2005-10-27</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.5°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft, Priism</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> PYE media (RB Jensen protocol);  5ml cultures in 14ml tubes; exponential phase; see DPD notebook Sep 28-30, 2005</p>
<strong>Sample Preparation:</strong> box 642, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -1, blot 1 sec, drain 1 sec; see DPD notebook Sep 30, 2005</p>
  pdd2005-10-27-1_slicer5352.jpg: S-layer .  Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/rawdata/</p>YB2837_05Oct27t01_n.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/rawdata/YB2837_05Oct27t01_n.mrc"
        > <i class="download icon"></i> Download </a></p>

        YB2837_05Oct27t01_p.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/rawdata/YB2837_05Oct27t01_p.mrc"
        > <i class="download icon"></i> Download </a></p>

        05Oct27t01bn_full.rec,  Reconstruction (Pixel Size 2.4 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/3dimage_263/05Oct27t01bn_full.rec"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-254y214.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_594/SAVE_x-254y214.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-254y214.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_596/SAVE_x-254y214.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-302y202.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_597/SAVE_x-302y202.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-302y202.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_599/SAVE_x-302y202.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-302y263.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_600/SAVE_x-302y263.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-302y263.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_602/SAVE_x-302y263.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-69y206.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_603/SAVE_x-69y206.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-69y206.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_605/SAVE_x-69y206.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-76y221.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_606/SAVE_x-76y221.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x-76y221.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_608/SAVE_x-76y221.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x142y333.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_609/SAVE_x142y333.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x142y333.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_611/SAVE_x142y333.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x147y-100.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_612/SAVE_x147y-100.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x147y-100.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_614/SAVE_x147y-100.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x162y-120.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_615/SAVE_x162y-120.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x162y-120.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_617/SAVE_x162y-120.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x68y-119.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_618/SAVE_x68y-119.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x68y-119.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_620/SAVE_x68y-119.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x68y-94.dm3,  file associated to 3D Image #263, 8.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_621/SAVE_x68y-94.dm3"
        > <i class="download icon"></i> Download </a></p>

        SAVE_x68y-94.tif,  file associated to 3D Image #263, 8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-27-1/file_623/SAVE_x68y-94.tif"
        > <i class="download icon"></i> Download </a></p>