DOI: 10.6084/m9.figshare.14650776.v1
title: Additional file 2 of Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue

description: Additional file 2: Tables containing additional information of models tested and data (re-)analysis. Table S1. Parameters, error (in parentheses), and pseudo-R2 for models of freezing damage as measured by A-D) electrolyte leakage or E) visual observation of cambial browning. Models are fit to A-C, E) a general logistic curve or D) a Gompertz curve as described in the text. Table S2. Maximum freezing-induced leakage (%) attained across several electrolyte leakage studies. Mean separations reflect outcomes of Tukey's HSD post-hoc tests (α = 0.05). A) Data for maple (Acer spp.) species included in the present study. Percentages indicate leakage following immersion in liquid nitrogen divided by leakage following boiling. Superscripts reflect species differences among means. B) Data for oak (Quercus spp.) species from Fallon and Cavender-Bares' [11] study. Percentages indicate leakage following freezing to -40℃ (the lowest freezing temperature used in the study) divided by leakage following boiling. Superscripts reflect differences among means of species-treatment combinations. C) Data for a variety of species assessed during three times of year extracted from supplementary figures from Kreyling et al. [10]. Percentages indicate leakage following immersion in liquid nitrogen divided by leakage following boiling. Two axes of mean separation are presented. Differences among species within the same sampling month are indicated by lowercase letters. Differences among sampling months for a single species are indicated by uppercase letters. Color coding (red to blue) highlights species and temporal patterns in maximum freezing-induced leakage in the Kreyling et al. data. Table S3. Results from Type III ANOVA of linear models assessing the consequences of species (A. caudatifolium vs. A. campestre), control type (boiling at 120C, boiling at 125 C, or liquid nitrogen immersion), and incubation time (1, 2, 5, or 7 days post-treatment) on A) conductivity after experimental freezing and B) conductivity after control treatment. Temperature of experimental freezing was included as a covariate.Samples were collected on 29 Dec. 2020. Table S4. Critical cold hardiness (T50, degrees C) estimated using the Limlogistic approach for two species, A) Acer caudatifolium and B) A. campestre with control type (boiling at 120C, boiling at 125 C, or liquid nitrogen immersion) and incubation time (1, 2, 5, or 7* days post-treatment) varied. Samples were collected on 29 Dec. 2020. Table S5. Species means of critical temperatures at which tissue accrued 20% and 80% damage as measured with electrolyte leakage and calculated using the Limlogistic approach. In cases for which differences among species are significant, superscripts indicate results of a Tukey's HSD post-hoc test (α = 0.05). Table S6. Description of all sampled plants. Genotypes of 10 species are all accessioned individuals in the Arnold Arboretum's living collections, as detailed below. Samples of A. negundo were collected from three spontaneously occurring plants found in the Arboretum's Bussey Brook Meadow. Samples of A. platanoides were similarly collected from three spontaneous individuals growing on the Arboretum's Weld Hill parcel.