cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.6592231
title: Modulation of Multidrug Resistance Protein 1-mediated transport processes by the antiretroviral drug ritonavir

description: We here provide the structure files, trajectories and topology files for analyzing and reproducing simulations performed in the publication: "<strong>Modulation of Multidrug Resistance Protein 1-mediated transport processes by the antiretroviral drug ritonavir</strong>". Classical molecular dynamics simulation of rat Mrp1 have been performed in complex with native substrates like GSH and GSSG or drug molecules like ritonavir, to study binding modes of different combinations. Structure and trajectory files (all 1 µs long) as well as recorded movies of the time progression are provided for the following simulation systems: 1. GSH bound to rat Mrp1 2. GSSG bound to rat Mrp1 3. Ritonavir bound to rat Mrp1 4. GSH + Ritonavir bound to rat Mrp1 5. GSSG + Ritonavir bound to rat Mrp1 Additionally itp files (GROMACS format) containing parameter information about the ligands GSH, GSSG and Ritonavir are provided for the force field CHARMM36m. Raw data of the homology modeling of rat Mrp1 by I-Tasser as well as Docking results produced via AutoDock4 are uploaded as zip directories.
DOI: 10.6084/m9.figshare.19920616
title: Nutritional status and food consumption of patients with Parkinson disease

description: ABSTRACT Background: Parkinson's disease (PD) is a neurodegenerative disease that affects food intake as the disease progresses. The assessment of nutritional status is essential to identify early nutritional deficits. Objective: To investigate the nutritional status and food intake of patients diagnosed with PD. Methods: This was a descriptive, cross-sectional, and quantitative study. A structured questionnaire with restricted questions was applied to identify the sociodemographic profile and anthropometric measurements were taken. To evaluate the disease stage, the modified Hoehn & Yahr scale was used. Food intake was evaluated using the Food Frequency Questionnaire (FFQ). The acquired information was organized in the Microsoft Office Excel 2010 program and statistical analysis was performed using the BioEstat 5.3 program. Results: The research was carried out with 40 patients; the majority was male (60%), over 60 years old (52.5%), single (55%), with a high school degree (42.5%), retirees (80%), and receiving one minimum salary (42.5%). Most were classified with eutrophic (42.5%) and overweight (42.5%). About food consumption, the most common foods were banana (60%), fish (52.5%), acerola (45%), and orange (32%). Among the high-risk foods, red meat (82.5%), fried foods (30%), heavy cream (25%), and filled sandwich cookies (25%) were the most common. Conclusions: Most patients had a high consumption of red meat and processed foods, with relevant consumption of some fruits and low consumption of grains and oilseeds. The disease stage was not associated with nutritional status.
DOI: 10.5281/zenodo.5647862
title: Modulation of Multidrug Resistance Protein 1-mediated transport processes by the antiretroviral drug ritonavir

description: We here provide the structure files, trajectories and topology files for analyzing and reproducing simulations performed in the publication: "<strong>Modulation of Multidrug Resistance Protein 1-mediated transport processes by the antiretroviral drug ritonavir</strong>". Classical molecular dynamics simulation of rat Mrp1 have been performed in complex with native substrates like GSH and GSSG or drug molecules like ritonavir, to study binding modes of different combinations. Structure and trajectory files (all 1 µs long) as well as recorded movies of the time progression are provided for the following simulation systems: 1. GSH bound to rat Mrp1 2. GSSG bound to rat Mrp1 3. Ritonavir bound to rat Mrp1 4. GSH + Ritonavir bound to rat Mrp1 5. GSSG + Ritonavir bound to rat Mrp1 Additionally itp files (GROMACS format) containing parameter information about the ligands GSH, GSSG and Ritonavir are provided for the force field CHARMM36m. Raw data of the homology modeling of rat Mrp1 by I-Tasser as well as Docking results produced via AutoDock4 are uploaded as zip directories.
DOI: 10.6084/m9.figshare.19905555
title: Enterococcus Spp. Survival Through the Use of Standard Protocol for Clostridium Sp. Isolation

description: ABSTRACT The genera Clostridium and Enterococcus are very different from each other, both morphologically and physiologically. Due to the high resistance by the sporulation capacity of Clostridium species, the thermal shock is a characteristic tool used for the isolation and identification of these microorganisms, this way, it would eliminate any other bacteria that did not present spores. The objective of this work is to show that Enterococcus sp. resist the temperature treatment and grow in culture media used for the isolation of Clostridium sp. For this, the present study initially attempted to identify reducing sulfite clostridia in poultry products, through the use of specific culture media and heat shock treatment. However, the PCR did not detect the presence of Clostridium sp. Then, sequencing of the 16S rDNA region was performed, which showed that the reducing sulfite colonies that were being isolated were, actually, Enterococcus spp. With this, some tests were carried out using different temperature and time combinations in the thermal shock, as well as the use of five different selective and differential culture media, in an attempt to eliminate any contaminants, but all without success, because these bacteria resisted to all modification. Therefore, the standard protocol for the isolation of bacteria of the genus Clostridium does not eliminate Enterococcus, which can lead to failures in the quantification and qualification of sulfite reducing microorganisms, a fact that can significantly affect food safety and animal health.
DOI: 10.6084/m9.figshare.19905555.v1
title: Enterococcus Spp. Survival Through the Use of Standard Protocol for Clostridium Sp. Isolation

description: ABSTRACT The genera Clostridium and Enterococcus are very different from each other, both morphologically and physiologically. Due to the high resistance by the sporulation capacity of Clostridium species, the thermal shock is a characteristic tool used for the isolation and identification of these microorganisms, this way, it would eliminate any other bacteria that did not present spores. The objective of this work is to show that Enterococcus sp. resist the temperature treatment and grow in culture media used for the isolation of Clostridium sp. For this, the present study initially attempted to identify reducing sulfite clostridia in poultry products, through the use of specific culture media and heat shock treatment. However, the PCR did not detect the presence of Clostridium sp. Then, sequencing of the 16S rDNA region was performed, which showed that the reducing sulfite colonies that were being isolated were, actually, Enterococcus spp. With this, some tests were carried out using different temperature and time combinations in the thermal shock, as well as the use of five different selective and differential culture media, in an attempt to eliminate any contaminants, but all without success, because these bacteria resisted to all modification. Therefore, the standard protocol for the isolation of bacteria of the genus Clostridium does not eliminate Enterococcus, which can lead to failures in the quantification and qualification of sulfite reducing microorganisms, a fact that can significantly affect food safety and animal health.
DOI: 10.6084/m9.figshare.19920617
title: Completeness of rapid assessments of medium and large mammal diversity in the northwestern Amazon in Colombia

description: ABSTRACT Rapid assessments are the most common information source on biodiversity in the northwestern Amazon in Colombia due to limited resources and logistic constraints. These assessments are essential for decision-making on environmental policies in this region, that has been strongly impacted by the transformation of its natural ecosystems. Several local camera-trapping rapid assessments of medium and large-sized mammals (MLM) have been conducted in the Colombian Amazon, but they are difficult to compare. We analyzed information of 16 of these rapid assessments of MLM to provide the first list of MLM in the northwestern Amazon in Colombia. We also evaluated the accuracy of four estimators (ICE, Chao-2, Jackknife-1, and Jackknife-2), and the minimum sampling effort for the estimation of MLM richness in local surveys in the region. We report 26 species of MLM for the Colombian Amazon (between five and 13 species per locality), which is an underestimation of MLM richness in the region. The best estimator of MLM richness was the Jackknife-1, due to its precision and the lower influence of singletons. We recommend a minimum sampling effort of 350 camera trap-days. Although rapid assessments do not allow a robust estimation of MLM richness, they record the most common species (or core species) per locality and their abundance variation. The evaluation of the effect of habitat transformation on MLM and the estimation of population parameters of rare species require more intensive studies.
DOI: 10.6084/m9.figshare.19920617.v1
title: Completeness of rapid assessments of medium and large mammal diversity in the northwestern Amazon in Colombia

description: ABSTRACT Rapid assessments are the most common information source on biodiversity in the northwestern Amazon in Colombia due to limited resources and logistic constraints. These assessments are essential for decision-making on environmental policies in this region, that has been strongly impacted by the transformation of its natural ecosystems. Several local camera-trapping rapid assessments of medium and large-sized mammals (MLM) have been conducted in the Colombian Amazon, but they are difficult to compare. We analyzed information of 16 of these rapid assessments of MLM to provide the first list of MLM in the northwestern Amazon in Colombia. We also evaluated the accuracy of four estimators (ICE, Chao-2, Jackknife-1, and Jackknife-2), and the minimum sampling effort for the estimation of MLM richness in local surveys in the region. We report 26 species of MLM for the Colombian Amazon (between five and 13 species per locality), which is an underestimation of MLM richness in the region. The best estimator of MLM richness was the Jackknife-1, due to its precision and the lower influence of singletons. We recommend a minimum sampling effort of 350 camera trap-days. Although rapid assessments do not allow a robust estimation of MLM richness, they record the most common species (or core species) per locality and their abundance variation. The evaluation of the effect of habitat transformation on MLM and the estimation of population parameters of rare species require more intensive studies.
DOI: 10.6084/m9.figshare.19920623
title: Putative poison gland in the thorny catfish Acanthodoras spinosissimus (Siluriformes: Doradidae)

description: ABSTRACT Acanthodoras is the only genus of catfish known to secrete a conspicuous and abundant milky-looking substance through an axillary pore located just below the base of the posterior cleithral process. Despite this remarkable feature, there is no published information on the anatomical structures that produce the secretion and its possible biological/ecological functions. Dissection and histological analysis of preserved specimens of A. spinosissimus revealed the presence of a saccular axillary gland with large, binuclear secretory cells, similar to those found in other poisonous catfish. Secretory cells near the lumen appear to lose nuclei and become filled with secretory products, possibly with proteinaceous elements, as indicated by their eosinophilic appearance. As far as we know, the saccular morphology of the gland appears to constitute a unique characteristic of Acanthodoras among Doradidae catfishes. Further studies are necessary to determine the chemical composition of the secretion, as well as its possible uses by the catfish in its natural environment.
DOI: 10.6084/m9.figshare.19920623.v1
title: Putative poison gland in the thorny catfish Acanthodoras spinosissimus (Siluriformes: Doradidae)

description: ABSTRACT Acanthodoras is the only genus of catfish known to secrete a conspicuous and abundant milky-looking substance through an axillary pore located just below the base of the posterior cleithral process. Despite this remarkable feature, there is no published information on the anatomical structures that produce the secretion and its possible biological/ecological functions. Dissection and histological analysis of preserved specimens of A. spinosissimus revealed the presence of a saccular axillary gland with large, binuclear secretory cells, similar to those found in other poisonous catfish. Secretory cells near the lumen appear to lose nuclei and become filled with secretory products, possibly with proteinaceous elements, as indicated by their eosinophilic appearance. As far as we know, the saccular morphology of the gland appears to constitute a unique characteristic of Acanthodoras among Doradidae catfishes. Further studies are necessary to determine the chemical composition of the secretion, as well as its possible uses by the catfish in its natural environment.
DOI: 10.6084/m9.figshare.19920626
title: A matter of quality: Kurt Goldstein‘s assessment of the abstract attitude after brain damage

description: ABSTRACT Neurologist and psychiatrist Kurt Goldstein (1878-1965) made substantial contributions to neuropsychology in general and to the development of tests for the assessment of brain damage sequelae in particular. Unlike present-day neuropsychology’s psychometric orientation, Goldstein kept a critical distance to a mere quantitative evaluation. Eighty years ago, he impressively demonstrated his own, qualitatively oriented diagnostic approach both in a remarkable monograph and in a didactic film, in collaboration with psychologist Martin Scheerer (1900-1961). By modifying a classical paradigm for the assessment of deficits in visuospatial construction, the Block Design Test, the two authors developed the Goldstein-Scheerer Cube Test. This version characterizes itself by offering the patient different types of cues in order to reveal the nature of the deficit at stake. The test remains an impressive illustration of Goldstein’s most famous neuropsychological concept, viz. the human ability to abstract from a concrete situation: the abstract (or categorial) attitude.
DOI: 10.6084/m9.figshare.19920626.v1
title: A matter of quality: Kurt Goldstein‘s assessment of the abstract attitude after brain damage

description: ABSTRACT Neurologist and psychiatrist Kurt Goldstein (1878-1965) made substantial contributions to neuropsychology in general and to the development of tests for the assessment of brain damage sequelae in particular. Unlike present-day neuropsychology’s psychometric orientation, Goldstein kept a critical distance to a mere quantitative evaluation. Eighty years ago, he impressively demonstrated his own, qualitatively oriented diagnostic approach both in a remarkable monograph and in a didactic film, in collaboration with psychologist Martin Scheerer (1900-1961). By modifying a classical paradigm for the assessment of deficits in visuospatial construction, the Block Design Test, the two authors developed the Goldstein-Scheerer Cube Test. This version characterizes itself by offering the patient different types of cues in order to reveal the nature of the deficit at stake. The test remains an impressive illustration of Goldstein’s most famous neuropsychological concept, viz. the human ability to abstract from a concrete situation: the abstract (or categorial) attitude.
DOI: 10.6084/m9.figshare.19920628
title: First record of helminths parasitizing Vanellus chilensis (Aves, Charadriiformes) in Acre state, southwestern Brazilian Amazon

description: ABSTRACT The southern lapwing, Vanellus chilensis, is a bird frequently seen in most of Brazil. Although it is widely distributed in other Brazilian biomes, including some parts of the Brazilian Amazon, it has only been recorded in the western Amazonian state of Acre since 2000. We report the presence of intestinal parasites in individuals of V. chilensis from the Cazumbá-Iracema Extractive Reserve in Acre through a coproparasitological analysis. Seven of the nine sampled individuals were parasitized. We observed larvae of Strongyloides sp. (28.5% of the samples), and eggs of Ascaridia sp. (28.5%), Ancylostoma sp. (14.2%), and Choanotaenia sp. (42.8%). These parasites are reported for the first time parasitizing V. chilensis in Brazil. The parasitized birds may act as reservoirs and were recorded in a peridomicile area, which may facilitate their contact with domestic birds.
DOI: 10.6084/m9.figshare.19920628.v1
title: First record of helminths parasitizing Vanellus chilensis (Aves, Charadriiformes) in Acre state, southwestern Brazilian Amazon

description: ABSTRACT The southern lapwing, Vanellus chilensis, is a bird frequently seen in most of Brazil. Although it is widely distributed in other Brazilian biomes, including some parts of the Brazilian Amazon, it has only been recorded in the western Amazonian state of Acre since 2000. We report the presence of intestinal parasites in individuals of V. chilensis from the Cazumbá-Iracema Extractive Reserve in Acre through a coproparasitological analysis. Seven of the nine sampled individuals were parasitized. We observed larvae of Strongyloides sp. (28.5% of the samples), and eggs of Ascaridia sp. (28.5%), Ancylostoma sp. (14.2%), and Choanotaenia sp. (42.8%). These parasites are reported for the first time parasitizing V. chilensis in Brazil. The parasitized birds may act as reservoirs and were recorded in a peridomicile area, which may facilitate their contact with domestic birds.
DOI: 10.6084/m9.figshare.19920631
title: Association between Leisure Time Physical Activity and HDL-C in the Elsa-Brasil Study Participants: Are There Any Gender Differences in the Dose-Response Effect?

description: Abstract Background High levels of high-density lipoprotein (HDL-C) are known for their protective effect against cardiovascular diseases and the regular practice of leisure time physical activity (LTPA) may be associated with their increase. Objective To verify the existence of differences between genders in the dose-response effect regarding the association between LTPA and HDL-C in the ELSA-Brasil study cohort. Methods Cross-sectional study with data from wave 2 of 13,931 participants of both genders (7,607 women) from the Longitudinal Study of Adult Health ELSA-Brasil. The LTPA was measured using the International Physical Activity Questionnaire (IPAQ) and classified into four categories: sedentary, low active, active and very active. The discriminatory power of LTPA at different intensities analyzed for high HDL-C was tested using ROC curves. Associations, adjusted for confounders between LTPA and HDL-C were analyzed by logistic regression. A 95% confidence interval was used. Results A positive association, with a dose-response effect, was observed between LTPA and HDL-C in both men and women. With regard to intensity, only vigorous physical activity discriminated high HDL-C in men, while both walking and moderate and vigorous physical activity discriminated high HDL-C in women. Conclusions LTPA shows a positive association with gradient dose-response and HDL-C, but in men, the association is not observed for those classified as physically unfit. In women, both walking intensity and moderate or vigorous physical activity can discriminate high HDL-C levels, whereas only vigorous intensity-exercise discriminate elevated HDL-C levels in men, demonstrating that males need to do more physical activity for this benefit to be observed.
DOI: 10.6084/m9.figshare.19920631.v1
title: Association between Leisure Time Physical Activity and HDL-C in the Elsa-Brasil Study Participants: Are There Any Gender Differences in the Dose-Response Effect?

description: Abstract Background High levels of high-density lipoprotein (HDL-C) are known for their protective effect against cardiovascular diseases and the regular practice of leisure time physical activity (LTPA) may be associated with their increase. Objective To verify the existence of differences between genders in the dose-response effect regarding the association between LTPA and HDL-C in the ELSA-Brasil study cohort. Methods Cross-sectional study with data from wave 2 of 13,931 participants of both genders (7,607 women) from the Longitudinal Study of Adult Health ELSA-Brasil. The LTPA was measured using the International Physical Activity Questionnaire (IPAQ) and classified into four categories: sedentary, low active, active and very active. The discriminatory power of LTPA at different intensities analyzed for high HDL-C was tested using ROC curves. Associations, adjusted for confounders between LTPA and HDL-C were analyzed by logistic regression. A 95% confidence interval was used. Results A positive association, with a dose-response effect, was observed between LTPA and HDL-C in both men and women. With regard to intensity, only vigorous physical activity discriminated high HDL-C in men, while both walking and moderate and vigorous physical activity discriminated high HDL-C in women. Conclusions LTPA shows a positive association with gradient dose-response and HDL-C, but in men, the association is not observed for those classified as physically unfit. In women, both walking intensity and moderate or vigorous physical activity can discriminate high HDL-C levels, whereas only vigorous intensity-exercise discriminate elevated HDL-C levels in men, demonstrating that males need to do more physical activity for this benefit to be observed.
DOI: 10.6084/m9.figshare.19920632.v1
title: Inhibitory control training in healthy and highly educated older adults

description: ABSTRACT. Executive function training is considered a promising tool for delaying the natural effects of aging on cognition. However, there are still few studies that propose a unimodal intervention with a focus on inhibitory control, and none of them has studied the effect of this type of intervention on older adults (OA). Objective: The aim of this study is to investigate the benefits of inhibitory control training in healthy OA by comparing the two assessment time points, namely, before and after training. Methods: Twenty-seven participants were included after interview and checking the inclusion criteria. The training was based on the stop-signal paradigm and carried out in 21 sessions. Results: Participants performed better after training by reducing the false alarm error rate (i.e., for stop-signal trials), reducing omission error rate, showing an increase in hit rate, Go response time (i.e., for go-signal trials), stop-signal response time, and showing a decrease in the level of anxiety. The executive function training had no significant impact on the scores obtained in the complementary neuropsychological tests. Conclusions: These results are consistent with previous studies that support the viability and effectiveness of cognitive intervention for executive functions in OA and suggest a positive effect of the intervention, which may be related to the learning experience of a new and challenging task.