DOI: 10.5061/dryad.hqbzkh1sg
title: Data from: Duration vs severity: Understanding invertebrate herbivory under varying warming events

description: As oceans warm, organisms are experiencing increased thermal stress.
 Marine thermal anomalies occur in both short-term heatwaves and long-term
 increases to mean temperatures, but direct comparative studies looking at
 the impacts of multiple types of thermal anomalies are rare. In
 ectothermic organisms, studies examining metabolic change after heatwaves
 show conflicting results to long-term climate change studies, leaving
 questions as to how species will be impacted across timescales. We tested
 the hypothesis that moderate experimental warming would increase herbivory
 rates over time, with a more pronounced increase occurring during
 long-term warming in comparison to short-term heatwaves. After simulating
 warming events on red sea urchins (Mesocentrotus franciscanus) and purple
 sea urchins (Strongylocentrotus purpuratus), the dominant eastern Pacific
 kelp forest herbivores, we found that feeding rates and thermal maxima
 varied by species and length of warming. While short-term heatwave events
 showed no increase in herbivory, longer-term warming led to increased
 herbivory in both species. Consumption and dissection data is included for
 both long-term and short-term warming experiments for both urchin species.
description: # Title: Duration vs severity: understanding invertebrate herbivory under
 varying warming events Author: Mary Cortese Finalized: 10.23.25 All Data
 was collected at the University of Washington's Friday Harbor
 Laboratory in Friday Harbor, Washington. More details provided in each
 data sheet. All weights are in grams File:
 "Heatwave_Data_Compiled.csv" About: Data collected during the
 heatwave experiment with grams of kelp consumed for each individual urchin
 within the heatwave experiment for each assay.Compiled data from all eight
 assays. For each date, data is the adjusted consumption data, meaning
 weight consumed minus the average change in weight of the controls held at
 the same temperature. See manuscript for more details on kelp controls.
 Note: NA would mean the urchin died and was not able to consume any kelp.
 Columns include: * Number: Urchin number (unique identifier) * Tank: the
 number of each large mesocosm tank * Temp: celsius, the maximum
 temperature of each tank * Species: red or purple depending on which
 urchin species, see manuscript for species name. * Assay 1-8: Amount of
 kelp consumed by urchin in grams corresponding to each feeding assay. *
 TestDiameter: test diameter measured before the experiment began (mm).
 File: "Intertidal_Subtidal_data_compiled.csv" About: Data
 collected during the intertidal/subtidal comparison experiment with grams
 of kelp consumed for each urchin during each individual assay.Compiled
 data from all four assays. For each date, data is the adjusted consumption
 data meaning the weight of kelp consumed minus the average change in
 weight of the controls held at the same temperature. See manuscript for
 more details on kelp controls. Note: NA would mean the urchin died and was
 not able to consume any kelp. Columns include: * Number: Urchin number
 (unique identifier). * Population: Inter for intertidal, sub for subtidal.
 * Species: red or purple depending on which urchin species, see manuscript
 for species name. * Tank: the number of each large mesocosm tank. * Date
 Oct 23- Nov 1: grams of kelp consumed per assay labeled by the first day
 of each assay. Year: 2022. * Average: average grams consumed across all
 four assays per individual. File:
 "LongTerm_Consumption_Data.csv" About: Data collected during the
 long term urchin consumption experiment with grams of kelp consumed for
 each urchin during each individual assay.Compiled data from all 22 assays.
 For each date, data is the adjusted consumption data. Note: Calculations
 for adjusted weight was tracked on a separate datasheet with other raw
 data and can be made avaiable upon request.Note: NA would mean the urchin
 died and was not able to consume any kelp. Columns include: *
 Urchin_Number: Urchin number (unique identifier). * Temp: the maximum
 temperature of each tank, celsius. * Tank: the number of each large
 mesocosm tank. * Species:red or purple depending on which urchin species,
 see manuscript for species name. * Dates Aug 29- Nov 1: grams of kelp
 consumed corresponding to the first day of each assay.Year: 2022. *
 Average_consumption: average grams consumed per individual across all 22
 assays. * TestDiameter: test diameter measured before the experiment began
 (mm). File:
 "Dissection_Data-_LongTerm_Experiment_Measurements.csv"
 About:Data from urchin dissection after the long-term experiment. Date of
 Dissection: 11/2/22, Date of dry weight: 11/6/22, Intertidal urchins
 collected from Clallum Bay WA on 8/9/2022. Goand Dry weight has one gonad
 removed for preservation and histology. Multiply by 1.2 for approximate
 dry weight. All weights are in grams. Data sheet for dissection data post
 long-term experiment. Columns include: * Urchin #: urchin unique
 identifier assigned upon placement in tank, corresponds to individual
 number in long term consumption datasheet. * SP: species, R for red, P for
 purple. * Diam 1: diameter of test in mm. * Diam 2: diameter of test,
 collected at a separate point, in mm. * Diam AVG: average diameter across
 both measurements, in mm. * Height 1: Height of test in mm. * Height 2:
 Height of test collected at a separate point, in mm. * WW Total: total wet
 weight of whole urchin in grams. * Test Cup #: unique identifier for each
 weigh boat. * Test Cup W: weight, in grams, of each weigh boat * Test WW:
 wet weight of dissected test (g). * Test DW: Dry weight of dissected test
 (g). * Test WW correct: wet weight of dissected test minus the weight of
 the corresponding weigh boat used (g). * Test DW correct: dry weight of
 dissected test minus the weight of the corresponding weigh boat used (g).
 * Lantern cup #: unique identifier for each weigh boat. * Lantern WW: Wet
 weight of dissected Aristotle's lantern (g). * Lantern DW: dry weight
 of dissected Aristotle's lantern (g). * Stomach Cup #: unique
 identifier for each weigh boat. * Stomach WW: wet weight of dissected
 stomach, including contents (g). * Stomach DW: dry weight of dissected
 stomach (g). * Gonad Cup #: Unique identifier for weigh boat. * Gonad Cup
 Weight: Weight of each weigh boat (g). * Gonad WW: wet weight of gonad
 (g). * Gonad DW: dry weight of gonad (g), one removed for preservation.
 See note above. * Gonad WW Correct: Gonad wet weight minus the weight of
 the weigh boat (g). * Gonad DW Correct: Gonad dry weight minus the weight
 of the weigh boat (g). * Gonad Index Corrected: Gonad index calculated
 with the corrected weights, GI = gonad WW/ Test WW. * Gonad Index V2:
 Gonad index calculated with corrected weights, GI = gonad WW/ Whole animal
 WW. File: "Dissection_Data-_LongTerm_Experiment_GonadIndex.csv"
 About:Data from urchin dissection after the long-term experiment. Date of
 Dissection: 11/2/22, Date of dry weight: 11/6/22, Intertidal urchins
 collected from Clallum Bay WA on 8/9/2022. Abridged datasheet for running
 gonad index analysis. Contains only calculated GI from master LongTerm
 Measurements Datasheet. Columns include: * Urchin: urchin unique
 identifier. * GonadIndex: gonad index calculation corresponding to
 'Gonad Index Corrected' column from LongTerm measurements sheet
 (gonad WW/ Test WW). * Temp: Maximum temperature of tank, degrees celsius
 * Tank: number of each mesocosm. * Species: purple or red. * GonadIndexV2:
 gonad index calculation corresponding to 'Gonad Index V2' column
 from LongTerm measurements sheet (GI = gonad WW/ Whole Urchin WW) File:
 "Dissection_Data-_LongTerm_Experiment_Change.csv" About:Data
 from urchin dissection after the long-term experiment. Date of Dissection:
 11/2/22, Date of dry weight: 11/6/22, Intertidal urchins collected from
 Clallum Bay WA on 8/9/2022. Datasheet calculating the change in body size
 of each urchin after the longterm experiment using various metrics.
 Columns include: * UrchinNumber: Unique identifier * OriginalDiam: Average
 diameter of urchin before experiment, mm. * EndDiam: Average diameter of
 urchin test after experiment, mm. * Diam Change: difference in urchin
 diameter before and after experiment (mm). * Original weight: wet weight
 of whole urchin before experiment, grams. * EndWeight: wet weight of whole
 urchin after experiment, grams. * WeightChange: differnece in wet weight
 before and after experiment (g). * Temp: Celsius, max temp of tank urchin
 was in. * Tank: Unique identifier for mesocosm urchin was in. * Species:
 red or purple. * CorrectedWeightChange: Change in ww divided by the
 original ww for percent change calculation. * WWPercentChange:
 'correctedWeightChange' Column multiplied by 100 to calculate
 percent change. File:
 "Dissection_Data-_PreExperiment_Measurements.csv" About: A group
 of urchins, collected around the same time and from the same population as
 the long-term experiment, were dissected without undergoing experimental
 treatment to assess body condition of urchins long-term experimental
 urchins upon collection. Intertidal urchins collected from Clallum Bay WA
 on 9/9/22. Date of Dissection: 9/12/22. Date of dry weight: 9/20/22.Data
 sheet for dissection data of non-experimental urchins. Columns include: *
 Urchin #: urchin unique identifier, assigned at random. Does no correspond
 to any other experimental urchins. * SP: species, R for red, P for purple.
 * Diam 1: diameter of test in mm. * Diam 2: diameter of test, collected at
 a separate point, in mm. * Height 1: Height of test in mm. * Height 2:
 Height of test collected at a separate point, in mm. * WW Total: total wet
 weight of whole urchin in grams. * Gonad Cup #: Unique identifier for
 weigh boat. * Gonad Cup Weight: Weight of each weigh boat (g). * Gonad WW:
 wet weight of gonad (g). * Gonad DW: dry weight of gonad (g). * Gonad WW
 Correct: Gonad wet weight minus the weight of the weigh boat (g). * Gonad
 DW Correct: Gonad dry weight minus the weight of the weigh boat (g). *
 Stomach Cup #: unique identifier for each weigh boat. * Stomach WW: wet
 weight of dissected stomach, including contents (g). * Stomach DW: dry
 weight of dissected stomach (g). * Lantern cup #: unique identifier for
 each weigh boat. * Lantern WW: Wet weight of dissected Aristotle's
 lantern (g). * Lantern DW: dry weight of dissected Aristotle's
 lantern (g). * Test Cup #: unique identifier for each weigh boat. * Test
 Cup Weight: weight, in grams, of each weigh boat (g). * Test WW: wet
 weight of dissected test (g). * Test DW: Dry weight of dissected test (g).
 * Test WW correct: wet weight of dissected test minus the weight of the
 corresponding weigh boat used (g). * Test DW correct: dry weight of
 dissected test minus the weight of the corresponding weigh boat used (g).
 * Gonad Index Corrected: Gonad index calculated with the corrected
 weights, GI = gonad WW/ Test WW. * Gonad Index V2: Gonad index calculated
 with corrected weights, GI = gonad WW/ Whole animal WW. File:
 "Dissection_Data-_PreExperiment_PrePost_Comparison.csv" About: A
 group of urchins, collected around the same time and from the same
 population as the long-term experiment, were dissected without undergoing
 experimental treatment to assess body condition of urchins long-term
 experimental urchins upon collection. Intertidal urchins collected from
 Clallum Bay WA on 9/9/22. Date of Dissection: 9/12/22. Date of dry weight:
 9/20/22.Sheet comparing Gonad index between the pre-experiment dissected
 urchins and the post-experimental treatment dissected urchins. Columns
 include: * Urchin: Unique identifier within each population. * GonadIndex:
 Corresponds to the 'Gonad Index Corrected' columns in both the
 pre and post dissection datasheets. * Temp: Max temp of each tank in
 Celsius. * Tank: Unique identifier for each mesocosm within each
 experiment. * Species: red or purple. * GonadWWCorrect: Gonad wet weight
 minus the weight of the weigh boat (g). * Status: Pre-Experiment or
 Post-Experiment. * GonadIndexV2: corresponds to 'Gonad Index V2'
 in both the pre experiment and post longterm datasheets.