DOI: 10.22002/eh3qz-6et89
title: Wild type NA1000

description: This is the cell shown in Fig.2(A-A3) of Li et al. 2007.
description: <strong>Tilt Series Date:</strong> 2006-03-23</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, Amira</p>
<strong>Collaborators and Roles:</strong> Cells were cultured by Zhuo Li. Data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle.</p>
<strong>Sample Preparation:</strong> 4 ul of cell culture at different time points were mixed with 10nm colloidal gold and plunge-frozen onto glow-discharged Quantifoil R2/2, R2/1, or R1.2/1.3 grids using a Vitrobot (FEI).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/3dimage_662/</p>dwt75min01.rec_0015.mrc,  Denoised tomogram, 104.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/3dimage_662/dwt75min01.rec_0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        wt75min23030663w185e12u1d01.mrc,  Tilt Series (Pixel Size 0.67 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/rawdata/wt75min23030663w185e12u1d01.mrc"
        > <i class="download icon"></i> Download </a></p>

        wt75min01_full.rec,  Reconstruction (Pixel Size 1.34 nm), 901.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/3dimage_742/wt75min01_full.rec"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01.mod,  Segmentation, 4.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1347/dwt75min01.mod"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01_ascii_ascii.am,  Segmentation, 2.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1348/dwt75min01_ascii_ascii.am"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01_ascii_asciiIM.am,  Segmentation, 2.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1349/dwt75min01_ascii_asciiIM.am"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01_ascii_asciiIM2.surf,  Segmentation, 324.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1350/dwt75min01_ascii_asciiIM2.surf"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01_ascii_asciiIM2.surf,  Segmentation, 324.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1351/dwt75min01_ascii_asciiIM2.surf"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01_ascii_asciiOM.am,  Segmentation, 2.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1352/dwt75min01_ascii_asciiOM.am"
        > <i class="download icon"></i> Download </a></p>

        dwt75min01_ascii_asciiOM2.surf,  Segmentation, 324.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1353/dwt75min01_ascii_asciiOM2.surf"
        > <i class="download icon"></i> Download </a></p>

        Fig9_0unconstricted.hx,  Segmentation, 4.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-03-23-4/file_1354/Fig9_0unconstricted.hx"
        > <i class="download icon"></i> Download </a></p>