cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647574.v1
title: CIL:34929

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell interior at cell edge (CIL 34926) of cytochalasin D-treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal ABP-280 at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green) can be found at (CIL 34923). Image corresponds to Figure 5h from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647568
title: CIL:34923

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles human 356 fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green). The protein/actin ratio at the leading edge of the lamellipodium is medium for ABP-280. Immuno-EM of the cell edge (CIL 34926) or interior (CIL 34929) of cytochalasin D treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody reveals ABP-280 at filament crossovers in the cell interior. Image corresponds to Figure 5b and b' from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647569
title: CIL:34924

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles of Xenopus fibroblast lamellipodia double stained with TRITC-phalloidin (red) and α-actinin antibodies (green). The protein/actin ratio at the leading edge of the lamellipodium is low for α-actinin compared with internal actin structures. Immuno-EM of the cell edge (CIL 34927) or interior (CIL 34930) of cytochalsin D-treated Xenopus fibroblasts stained with α-actinin ( primary antibody and 18-nm gold-conjugated secondary antibody) reveals α-actinin at filament crossovers in the cell interior. Image corresponds to Figure 5c and c' from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647570.v1
title: CIL:34925

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 (Arp2/3) primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal Arp2/3 complex at Y-junctions at cell edge. Fluorescence microscopy and corresponding intensity profiles double stained with TRITC-phalloidin (red) and p21 antibodies (green) is available at CIL 34922. Image corresponds to Figure 5d from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647571.v1
title: CIL:34926

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34929) of cytochalasin D-treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal ABP-280 at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green) can be found at (CIL 34923). Image corresponds to Figure 5e from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647566
title: CIL:34886

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1f from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647567
title: CIL:34887

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1g from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647568.v1
title: CIL:34923

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles human 356 fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green). The protein/actin ratio at the leading edge of the lamellipodium is medium for ABP-280. Immuno-EM of the cell edge (CIL 34926) or interior (CIL 34929) of cytochalasin D treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody reveals ABP-280 at filament crossovers in the cell interior. Image corresponds to Figure 5b and b' from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647567.v1
title: CIL:34887

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1g from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647562
title: CIL:25706

description: MTLn3 cell cotransfected with control siRNA and GFP-cortactin to visualize invadipodia dynamics. The corresponding FAK knockdown experiments are CIL:25705 and CIL:25704. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Video corresponds to Fig. 1 D. and supplemental video 1 in J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13. Bar, 10 µm.
DOI: 10.6084/m9.figshare.647564
title: CIL:34884

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1d from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647565
title: CIL:34885

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1e from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647566.v1
title: CIL:34886

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1f from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647563
title: CIL:34883

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1c from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647564.v1
title: CIL:34884

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1d from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647565.v1
title: CIL:34885

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1e from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647560
title: CIL:25704

description: MTLn3 cell cotransfected with FAK siRNA (target B) and GFP-cortactin to visualize invadipodia dynamics. The corresponding FAK control experiments is CIL:25706 and CIL:25705 shows a different FAK siRNA target. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Video corresponds to Fig. 1 D. and Supplementary Video 3 in Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13. Bar, 10 µm.
DOI: 10.6084/m9.figshare.647561
title: CIL:25705

description: MTLn3 cell cotransfected with FAK siRNA (target A) and GFP-cortactin.The corresponding FAK control experiment is CIL:25706, and an different target FAK siRNA experiment is CIL:25704. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Video corresponds to Fig. 1 D. and Supplemental Video 2 in J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13. Bar, 10 µm.
DOI: 10.6084/m9.figshare.647562.v1
title: CIL:25706

description: MTLn3 cell cotransfected with control siRNA and GFP-cortactin to visualize invadipodia dynamics. The corresponding FAK knockdown experiments are CIL:25705 and CIL:25704. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Video corresponds to Fig. 1 D. and supplemental video 1 in J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13. Bar, 10 µm.
DOI: 10.6084/m9.figshare.647563.v1
title: CIL:34883

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image show an enlargement of a local region from the overview of the leading edge, CIL 24786. Image corresponds to Figure 1c from J Cell Biol. 1999 May 31;145(5):1009-26. Figure 1b-1g are CIL 24882-24887.
DOI: 10.6084/m9.figshare.647559
title: CIL:25629

description: This movie illustrates the early division up to the four-cell stage of a wild-type C. elegans embryo by DIC microscopy. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1B and Movie 2 of Mouysset et al. Movie is CIL 25629 and original data file is CIL 35124.
DOI: 10.6084/m9.figshare.647561.v1
title: CIL:25705

description: MTLn3 cell cotransfected with FAK siRNA (target A) and GFP-cortactin.The corresponding FAK control experiment is CIL:25706, and an different target FAK siRNA experiment is CIL:25704. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Video corresponds to Fig. 1 D. and Supplemental Video 2 in J Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13. Bar, 10 µm.
DOI: 10.6084/m9.figshare.647557
title: CIL:24807

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes done with immuno-EM. A higher mag view of a localized region of the cell is shown in CIL 24808. Image corresponds to Figure 8g from J Cell Biol. 1999 May 31;145(5):1009-26. A fluorescence image showing XAC and phalloidin labelin is available as CIL 24807.
DOI: 10.6084/m9.figshare.647560.v1
title: CIL:25704

description: MTLn3 cell cotransfected with FAK siRNA (target B) and GFP-cortactin to visualize invadipodia dynamics. The corresponding FAK control experiments is CIL:25706 and CIL:25705 shows a different FAK siRNA target. Fluorescence imaging was performed using a 60×/1.40 oil objective on an inverted microscope (1X-70;Olympus) in a 37°C closed system as previously described. Glass-bottomed dishes (35 mm) were coated with 10 µg/ml fibronectin for 1 h at 37°C. Cells were plated in Ham’s F12 containing 5% FBS and 20 mM Hepes, pH 7.2, and were allowed to adhere for 3 h. Fluorescent images were collected using a cooled CCD camera (CoolSNAP FX; Hamamatsu Photonics) and captured into MetaVue every 1 min for 1 h. Time-lapse sequences from live fluorescence imaging were first subjected to high-pass filtration based on the water algorithm (Zamir et al., 1999) to remove diffuse background fluorescence. Video corresponds to Fig. 1 D. and Supplementary Video 3 in Cell Biol. 2009 Apr 20;185(2):357-70. Epub 2009 Apr 13. Bar, 10 µm.
DOI: 10.6084/m9.figshare.647556
title: CIL:24797

description: Localization of Arp2/3 complex in the lamellioidia of afibroblast . Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization, also in fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3h from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647558
title: CIL:24808

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes done with immuno-EM. A low mag view of the cell from which this high mag view is taken is shown in CIL 24807. Image corresponds to Figure 8h from J Cell Biol. 1999 May 31;145(5):1009-26. A fluorescence image showing XAC and phalloidin labeling is available as CIL 24807.
DOI: 10.6084/m9.figshare.647558.v1
title: CIL:24808

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes done with immuno-EM. A low mag view of the cell from which this high mag view is taken is shown in CIL 24807. Image corresponds to Figure 8h from J Cell Biol. 1999 May 31;145(5):1009-26. A fluorescence image showing XAC and phalloidin labeling is available as CIL 24807.
DOI: 10.6084/m9.figshare.647559.v1
title: CIL:25629

description: This movie illustrates the early division up to the four-cell stage of a wild-type C. elegans embryo by DIC microscopy. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1B and Movie 2 of Mouysset et al. Movie is CIL 25629 and original data file is CIL 35124.
DOI: 10.6084/m9.figshare.647555
title: CIL:24796

description: Localization of Arp2/3 complex in a fibroblast lamellioidia. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24796 shows an immuno EM image of Arp2/3 localization in a fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3e-g from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647556.v1
title: CIL:24797

description: Localization of Arp2/3 complex in the lamellioidia of afibroblast . Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization, also in fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3h from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647557.v1
title: CIL:24807

description: Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes done with immuno-EM. A higher mag view of a localized region of the cell is shown in CIL 24808. Image corresponds to Figure 8g from J Cell Biol. 1999 May 31;145(5):1009-26. A fluorescence image showing XAC and phalloidin labelin is available as CIL 24807.
DOI: 10.6084/m9.figshare.647553
title: CIL:24794

description: Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24795 shows an immuno EM image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3a-c from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647554
title: CIL:24795

description: Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3d from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647552
title: CIL:24788

description: Multiple branching of actin filaments in lamellipodia of vertebrate fibroblasts. This image shows an overview of the leading edge, and CIL 24789 shows enlargements of local regions of this platinum replica. CIL 24786 shows similar overview and local enlargements for the lamellipodia of a Xenopus keratocyte. Image corresponds to Figure 1h from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647553.v1
title: CIL:24794

description: Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24795 shows an immuno EM image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3a-c from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647554.v1
title: CIL:24795

description: Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3d from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647551
title: CIL:24786

description: Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image shows an overview of the leading edge, and CIL 24787 shows enlargements of local regions of this platinum replica. CIL 24788 shows a similar overview for the lamellipodia of a vertebrate fibroblasts. Image corresponds to Figure 1a from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647552.v1
title: CIL:24788

description: Multiple branching of actin filaments in lamellipodia of vertebrate fibroblasts. This image shows an overview of the leading edge, and CIL 24789 shows enlargements of local regions of this platinum replica. CIL 24786 shows similar overview and local enlargements for the lamellipodia of a Xenopus keratocyte. Image corresponds to Figure 1h from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647555.v1
title: CIL:24796

description: Localization of Arp2/3 complex in a fibroblast lamellioidia. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24796 shows an immuno EM image of Arp2/3 localization in a fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3e-g from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647548
title: CIL:23051

description: MAP4 is not required for plectin binding to microtubules (MTs). Electron microscopy of control human 356 fibroblasts (CIL:23051, this image) and MAP4 immunodepleted (CIL:23052) cells after staining with antibody to NH 2 terminus of MAP4. Cells were microinjected with an antibody to the COOH-terminal domain of MAP4 to remove MAP4 from MTs. 10-nm gold particles decorate MTs in control cells, but are absent in injected cells. Plectin forms bridges between intermediate filaments and MTs in both cells. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 9c in J Cell Biol. 1996 Nov;135(4):991-1007.
DOI: 10.6084/m9.figshare.647549
title: CIL:23052

description: MAP4 is not required for plectin binding to microtubules (MTs). Electron microscopy of control human 356 fibroblasts (CIL:23051) and MAP4 immunodepleted (CIL:23052, this image) cells after staining with antibody to NH 2 terminus of MAP4. Cells were microinjected with an antibody to the COOH-terminal domain of MAP4 to remove MAP4 from MTs. 10-nm gold particles decorate MTs in control cells, but are absent in injected cells. Plectin forms bridges between intermediate filaments and MTs in both cells. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 9d in J Cell Biol. 1996 Nov;135(4):991-1007.