cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2017.338
title: NEW LOCATION RECOMMENDATION TECHNIQUE ON NETWORK

description: Nowadays, recommender systems play a crucial role in our daily life as can be seen from numerous websites that utilize the systems to recommend interesting items for their visitors, such as lonelyplanet.com, netfliex.com etc. From operational viewpoint, the recommender system is embedded in several domains by which it is categorized based on their usage. For example, lonelyplanet.com is categorized as a Location-Domain while netflex.com is in a Movie-Domain. In fact, the interesting attractions from traveling websites or movie selections from movie online sites are part of the recommendation outcome. To analyze the user’s preferences, many methods have been widely used including Content-based filtering and Collaborative filtering. Nevertheless, both methods have different benefits and drawbacks. As a result, a hybrid recommender system using more than one technique has been proposed to supplement the short-coming of one technique by the strength of the other technique. Additionally, with the popularity of social network, the new social filtering technique has also been deployed to search for the impact among users on the social network. This thesis proposed a novel method to recommend tourist attractions for the visitors implemented on the location-domain with the combination of 3 recommender system techniques: Content-based filtering, Collaborative filtering, and social-filtering. The comparative results show that the proposed method yields better performance of coverage result and NDCG average score than the other two methods.
DOI: 10.14457/cu.the.2017.339
title: HIGH CAPACITY IMAGE STEGANOGRAPHY TOLERATING IMAGE COMPRESSION

description: Nowadays, data privacy becomes more important. Most of the existing data protection schemes based on cryptography cannot hide the existence of secret data. Thus, a steganography approach plays an important role to handle this problem. Image steganography can be categorized into two methods which are baesd on spatial domain and transform domain. Methods using spatial domain have high embedding capacity but they are not robust to image compression. On the other hand, methods using transform domain usually work with image compression but have low embedding capacity. In recent image steganography research, many transform domain methods are based on JPEG format which is quite old and its compression efficiency may not be suitable in cloud era. In 2010, Google introduced WebP, a new image format that has better lossy compression than JPEG. In this study, the new image steganography based on WebP is presented. Re-calculation and LSB on middle-frequency method are used. The proposed scheme provides better image quality and smaller file size than the existing JPEG based method at the same capacity. Moreover, not only applying to a grayscale image but also a color image and an alpha channel color image is introduced in this research.
DOI: 10.14457/cu.the.2017.340
title: Threshold of luminance contrast and chromaticity contrast for subjects wearing simulated low vision glasses

description: As reported by WHO in 2010, there are 246 million people with low vision (total population in the world is 6,737 million). The proper environment is important for doing their daily activities not only to be safe and convenient but also to improve the quality of life. This research investigated the threshold of luminance contrast and chromaticity contrast for subjects wearing simulated low vision glasses. The subjests are color normal vision wearing simulated low vision glasses with visual acuity (VA) ranging between 0.05 and 0.3: narrow vision (NV), blur vision (BL), occlusion vision (OLS) and combination of blur and occlusion vision (BL-OLS). They took part in series of psycho-physical experiments. The stimuli are achromatic and chromatic sinusoidal gratings of different spatial frequencies and hues. For all stimulated low vision glasses at higher spatial frequencies, the luminance and chromaticity contrast thresholds were high compared to lower spatial frequencies except for blue and red in maxNV glasses. Yellow showed the highest chromaticity contrast threshold and they were not much different for all spatial frequencies. The medBL-maxOLS glasses showed the highest luminance and chromaticity contrast thresholds while the maxNV glasses showed the lowest ones.
DOI: 10.14457/cu.the.2017.341
title: THE ROLE OF TRIARYLMETHANE-34 ON POTASSIUM CHANNEL KCA3.1 IN DOXORUBICIN-INDUCED FELINE KIDNEY CELL LINE

description: Study involved with potassium channel KCa3.1 blocker (triarylmethane-34; TRAM-34) in doxorubicin (DOX)-induced feline kidney cells was performed. The study was divided into two parts. In part I, subtoxic dose of TRAM-34 in feline kidney cell lines was studied to determine the cytotoxicity of TRAM-34 in feline kidney cells by cytotoxicity assay. In part II, feline kidney cell lines were incubated with appropriate dose and time of TRAM-34 pretreatment for 24 h or 30 min and post-treatment for 24 h condition in DOX-induced cell toxicity. Cytotoxicity assay, apoptosis and necrosis assay and KCa3.1 protein expression were measured. The cytotoxicity results indicated no significantly differences in cell viability between cells treated with TRAM-34 at 0.1 to 100 µM concentration and negative control in 24 h but a significant reduction of cell surviving at 100 µM concentration of TRAM-34 in 48 h (p&lt;0.05). We found subtoxic dose of TRAM-34 in feline kidney cells lines at the concentration 100 µM in 24 h, 50 µM in 48 h and 25 µM in 96 h when compared with the negative control. Pretreatment with TRAM-34 at 0.1 to 1 µM concentrations for 24 h had significantly higher percentages of cell viability (p&lt;0.05) and significantly lower percentages of apoptotic cells respect to the total than DOX-treated control (p&lt;0.05) but was not significantly different in percentages of necrosis cells than DOX-treated control. Moreover, Pretreatment with TRAM-34 at the 0.1 µM concentrations for 24 h had significantly decreased KCa3.1 protein expression when compared with DOX-treated control (p&lt;0.05). Therefore, these findings suggested that TRAM-34 can protect feline kidney cells line from DOX-induced toxicity by inhibiting KCa3.1 channel. KCa3.1 channel blocker may be used as one of the potential therapeutic treatment for cats with naturally-occurring chronic kidney disease in the future.
DOI: 10.14457/cu.the.2017.342
title: Fatigue study of cancellous and cortical stainless steel screws used with polymethymethacrylate and the SOP plate system for the immobilisation of vertebral fracture and luxation in canine cadavers

description: Globally, vertebral fracture and luxation (VFL) are one of the most common neurological injuries in dogs and cats. The standard care for vertebral body stabilisation of canine VFL are screws with polymethylmethacrylate (PMMA) and the string of pearls (SOP) plate system fixation. The VFL with implants are usually exposed to force in a repetitive or cyclic loading fashion. Indeed, stress on the implant cyclically at a load significantly less than its ultimate tensile strength can cause fatigue failure, one of the major causes of implant breakage. This study aims to compare the failure mode of four different implants using a specifically designed fatigue testing machine. The four different implants were constructed as follows: 3.5 mm cancellous screw with manually applied PMMA (MP), 3.5 mm cancellous screws with syringe application of PMMA (CanP), 3.5 mm cortical screws with syringe application of PMMA (CorP) and 3.5 mm cortical screws with a SOP plate (SOP). The results revealed that the SOP group tolerated the most cycles before failure, followed by the CorP, CanP and MP groups repectively. The point of weakness, as defined by the failure mode, occurred in the middle of the PMMA bridge, 3.5 mm cancellous screw neck, PMMA at the screw neck position and 3.5 mm cortical screw in MP, CanP, CorP and SOP groups respectively. In conclusion, based on fatigue properties of the four implants tested in this study, the SOP plate is recommended as a standard fixation device for VFL in dogs.
DOI: 10.14457/cu.the.2017.343
title: Application of autologous blood-derived platelet-rich fibrin for periodontitis treatment in dogs

description: Periodontitis is one of the most prevalent inflammatory oral diseases in dog. Non-surgical managements are conventionally performed to obtain healthy oral status. With progressive periodontitis, advanced periodontal surgery and also dental extraction become warranted with major disadvantages of trauma, bleeding, prolonged anesthetic time and hospitalization, and wound healing impairment. Hence, use of platelet-rich fibrin has been studied and considered one of the novel approaches to augment in periodontitis management. This study aimed to evaluate the efficacy of autologous blood-derived platelet-rich fibrin in an aspect of periodontitis treatment in a canine models. In this study, the experiment was done at maxillary 4th premolar and mandibular 1st molar. Dogs with healthy oral status were served as a control (C group, n =5). Another 10 systemically healthy subjects presenting equally bilateral periodontitis were randomized into split mouth clinical design. Experimental groups included periodontitis group with conventional open-flap debridement (PD group, n = 20) and periodontitis group with conventional open-flap debridement and platelet-rich fibrin treatment (PD+ group, n = 20). Clinical parameters included plaque index (PI), gingival index (GI), periodontal pocket depth (PPD), and mobility index (MI) were evaluated at baseline, 7, 14, 21, and 56 days post experiment (DPE). Intra-oral radiography was undergone to assess alveolar bone at baseline, 21 and 56 DPE. Histopathological analysis concerning inflammatory and fibrosis score was evaluated at baseline and 14 DPE. Cytokine expression analysis via anti-inflammatory (TGF-β1, PDGF-B, VEGF-A, TIMP-1, COL1A1, and COL3A1) and pro-inflammatory cytokines (TNF-α and IL-1β) were evaluated at baseline, 7 and 14 DPE. The results revealed that PD+ group presented significant improvement in GI (at 14 DPE), PPD (at 21 and 56 DPE), and inflammatory reaction score (at 14 DPE) compared with PD group. Also, significant up-regulation of anti-inflammatory cytokines (except for COL1A1) together with down-regulation of pro-inflammatory cytokines were observed. In conclusion, platelet-rich fibrin might be an alternative novel approach for periodontitis treatment in dogs. (n represented surgical site)
DOI: 10.14457/cu.the.2017.344
title: SEROLOGICAL STATUS OF PCV2-INFECTED SWINE HERDS

description: This study aimed to determine the dynamics of serological and virological profiles against Porcine Circovirus type 2 (PCV2) infection in Thai swine herds using the in-house indirect ELISA and real-time PCR techniques. A total of 10 swine herds in Thailand were divided into two groups according to their clinical history. Group A (n=5) had clinical signs of PCV2, and was routinely vaccinated by PCV2 vaccines with production loss more than 5%. Group B (n=5) had no clinical signs of PCV2 with production loss less than 5%. Group B consisted of two subgroups: PCV2-vaccinated herds (B-Vac, n=2) and non-vaccinated herds (B-non-Vac, n=3). Serum samples (n=500) were collected from parity 1, 3 and 5 sows and at 3, 5, 9, 13, 17, 21 and 25 weeks of pigs (n=5 per age group). The result of seroprofiles of group A and B revealed similar pattern in which high antibody titers in all parity sows. However, PCV2 antiboby titers of group A at parity 1 and 5 sows were significantly higher than those of group B (p&lt;0.05). In piglets, the titers were high at 3 weeks and gradully decreased at 5 to 9 weeks of age indicating the decline in maternal immunity. After 9 weeks, PCV2 antibodies were gardually increased until fattening period coincided with high viral loads and high percentage of production loss indicating natural infection. At 17 weeks of age, the titers of group A were significantly higher than those of group B (p&lt;0.05). In group B, the antibody levels at 3 to 13 weeks of B-Vac were significantly lower than those of B-non-Vac. The detection of PCV2 load revealed that mean viral load of group A was higher than group B but it was not significantly different. Within group B, the PCV2 DNA load in B-Vac group was significantly lower than B-non-Vac group indicating that vaccination in non-clinical pigs could reduce viral load and risk of clinical disease. This study showed that in-house ELISA and detection of viral DNA together with PCV2 clinical expressions of pigs could indicate the dynamic of PCV2 infection within swine herds and could be beneficial for disease control.
DOI: 10.14457/cu.the.2017.345
title: Oral fluid samples used for PRRS diagnosis and management

description: Porcine reproductive and respiratory syndrome (PRRS) is an economically significant swine disease having a negative impact to the swine industry worldwide. Appropriate management strategies and diagnostic tests are crucial to successfully control PRRS virus (PRRSV). However, the application for oral fluid-based diagnosis has not been evaluated in the Thai farms previously. In this study, pre-extraction methods of oral fluids were evaluated to improve the PCR product yielded. Moreover, the oral fluids utilization for PRRSV monitoring during the gilt acclimatization and farrowing to nursery period was investigated. The results demonstrated that increasing sample volume might be a suitable simple method for column-based extraction kit to improve PRRSV detection. Whereas, other modified pre-extractions could possibly impact nucleic acid quality in the samples. In addition, field evaluation demonstrated that oral fluid testing provided convenient, economical, satisfied and animal welfare friendly method of sample collection with some limitations. Significant positive correlation was found between oral fluid results and the results from serum samples. Mean S/P ratios of oral fluid samples showed significantly higher levels than those of from the serum samples with similar patterns. During acclimatization period, oral fluid testing could be used to monitor the success of acclimatization. However, it did not completely monitor all PRRSV infection in acute phase of infection. In the farrowing to nursery study, oral fluid testing provided satisfied performance to determine PRRS status especially when having moderate to high prevalence. The changes in anti-PRRSV antibodies status in oral fluids after horizontal exposure were concurrently with the presence of high viral loads. In conclusion, oral fluid testing is a suitable sample for PRRSV monitoring. Additionally, the baseline and application for oral fluid-based diagnosis are of essential to explore on its utilization. The finding data would beneficial to the farmers and the future utilization for successful PRRS prevention and control.
DOI: 10.14457/cu.the.2017.346
title: Development of DNA vaccine against porcine epidemic diarrhea virus

description: Porcine epidemic diarrhea (PED) is a devastating enteric disease mainly affected to the sucking piglets which causing the severe economic losses in many countries worldwide including Thailand. To date, there have no satisfied commercial vaccine for PED. The clustering system based on the spike gene demonstrated 2 variant of PEDV, classical and pandemic variant, which the difference of these variants may be affected to the protective immunity between them. The objectives of this study were to investigate the genetic diversity of S gene of PEDV in Thailand and evaluate the efficacy of DNA vaccine which developed from Thai PEDV isolate. In this study, the nucleotide and amino acid sequence of S gene of Thai isolates PEDV were analyzed with isolates from other countries. The results demonstrated Thai PEDV isolates were belong to the pandemic variant which the neutralizing epitope of these isolates were different to the vaccine isolate. In addition, insertion and deletion at N-terminal of S gene were detected in these Thai isolates. To develop the DNA vaccine against PEDV, plasmid DNA was constructed and the vaccine efficacy was evaluated in weaned pigs and gestation gilts. Humoral and cellular immunity were investigated by viral neutralization assay, ELISA, lymphocyte proliferation and detection of IFN-g producing cells. The results demonstrated the absence of PEDV-specific antibody in all groups but the lymphocyte proliferation of CD4+ cell at 7 DPV and the PEDV-specific IFN-g producing cell of CD4+ and CD8+ cells at 28 DPV in vaccinated pigs was higher than control pigs (p&lt;0.05). Meanwhile, the PEDV-specific IgA and the neutralizing antibody titer in colostrum and milk of vaccinated sows were relatively higher than control group which might be associated with the booster effect. This study reveal the possibility of the DNA vaccine to be a vaccine candidate which might be used in the control and prevention program
DOI: 10.14457/cu.the.2017.347
title: Dual infection between a Thai isolate highly pathogenic porcine reproductive and respiratory syndrome virus and pandemic H1N1swine influenza virus in weanling pigs

description: After an introduction of pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in the Thai swine farms, mild clinical outcome is observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in the Thai swine farms. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups: Negative, PRRSV, SIV and Co-infection groups. Pigs in PRRSV and Co-infection groups were challenged intranasally with the Thai isolate HP-PRRSV at 0 days post HP-PRRSV challenge (dphc) and pigs in SIV and Co-infection groups were inoculated intratracheally with pdmH1N1 SIV at 6 dphc. The 1st and 2nd necropsies were done at 2 and 4 days post SIV challenge (dsc). A comparison of the lung lesion scores between PRRSV and Co-infection groups at 2 dsc showed no statistical difference, but at 4 dsc, lung lesions score of the Co-infection group had higher average score than that of the PRRSV only group. Also, SIV viral load in the lung of the Co-infection group showed higher viral load at 4 dsc, comparing to the SIV-infected group significantly. The results suggest that the low pathogenic pdmH1N1 SIV could enhance the lung severity when co-infected with the HP-PRRSV possibly leading to porcine respiratory disease complex (PRDC).
DOI: 10.14457/cu.the.2017.348
title: THE EFFECT OF DEXMEDETOMIDINE IN COMBINATION WITH PETHIDINE ON ISOFLURANE MINIMUM ALVEOLAR CONCENTRATION FOR CANINE ANTINOCICEPTION

description: The effects of dexmedetomidine combined with pethidine on the minimum alveolar concentration (MAC) of isoflurane and cardiorespiratory variables in dogs were evaluated in thirty client-owned, healthy adult male dogs scheduled for castration at the Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University. The dogs were allocated into Group 1 (n=6) receiving sterilized water for injections (placebo) as a negative control group, Group 2 (n=12) receiving 5 µg kg-1 of dexmedetomidine IM as a positive control group, and, Group 3 (n=12) receiving 5 µg kg-1 of dexmedetomidine and 5 mg kg-1 of pethidine IM. At 15 minutes after injection of the tested drug, sedation and pain response to clamping of the 3rd or 4th digit of the hindimb were evaluated. Then, anesthesia was induced via a face mask with 4% isoflurane in 4 L min-1 of oxygen. The ET isoflurane had been initially set at 1% for at least 15 min for anesthetic equilibration before the noxious stimulation was carried out by clamping the 3rd or 4th digit of the hind limb using a 20-cm hemostat. A positive response was considered when there was gross purposeful movement of the head or extremities. Once the response was positive or negative, the ET isoflurane concentration was increased or decreased by 0.1 - 0.2%, respectively. The new ET concentration was maintained for at least 15 min for anesthetic equilibration before the noxious stimulation was repeated. The isoflurane MAC was the average concentration of isoflurane between the highest ET concentration at which the purposeful movement was detected and the lowest ET concentration at which the movement was not detected. After determining the isoflurane MAC, the animal was castrated. Respiratory rate, heart rate, systolic arterial pressure, and venous blood gas variables were monitored before and after injection of the tested drug. Sedation scores after dexmedetomidine given alone and in combination with pethidine were significantly higher than that after the placebo injection, and the score after the combination was significantly greater than that after dexmedetomidine given alone. All dogs responded to the noxious stimulation before anesthesia induction. The isoflurane MAC of Groups 2 and 3 were significantly less than that of Group 1 (p &lt;0.05), and the isoflurane MAC of Group 3 was significantly less than that of Group 2. Cardiorespiratory variables except the heart rate were within the clinically acceptable limits. In conclusion, dexmedetomidine given with pethidine provided sedation and sparing effect on the isoflurane MAC significantly greater than dexmedetomidine given alone.
DOI: 10.14457/cu.the.2017.350
title: Effects of short-term growth hormone administration on plasma leptin levels in normal and diet-induced obesity rats

description: Obesity has related to the alteration of hormonal profiles, i.e. growth hormone (GH), insulin and leptin. Because of the pleiotropic mechanisms of GH, it plays important roles both in the pathophysiological mechanisms and the therapeutic hormonal supplementation, which probably affected plasma leptin. The present research aimed firstly to demonstrate the effect of short-term GH administration on plasma leptin in 3 conditions; basal, meal-induced and fasting condition, from the rats with different adipose tissue mass. Secondly, to investigate short-term effect of GH on insulin sensitivity and body adiposity in control, diet resistant (DR) and diet-induced obesity (DIO) rats. In this regard, the rats were divided to control and hypercaloric (HC) diet-feeding rats. After 6 weeks of feeding period, HC diet-feeding rats were selected for DR and DIO rats. Exogenous GH (1 mg/kg, twice daily) was injected and compared with saline-treated rats. Short-term GH treatment decreased plasma leptin only in DIO rats that significantly occurred at 32 h after the first GH injection. The leptin response probably depended on the higher body adiposity in DIO rats than that of control and DR rats. For meal-induced plasma leptin, short-term GH treatment had no effect in all rats. For fasting condition, GH treatment attenuated the effect of fasting on plasma leptin in control and DR rats, which had the lower adiposity than that of DIO rats. Insulin resistance (IR) was induced by short-term GH treatment, which demonstrated by the higher fasting insulin and increased surrogate indexes of insulin resistance in DR rats, including the homeostasis model IR and adipose tissue IR. Therefore, we conclude that the effect of short-term GH treatment on plasma leptin might depend on body adiposity and energy status. These findings reveal the evidence of short-term GH treatment on plasma leptin and may suggest the short-term GH treatment as adjunctive therapy in obese stage.