DOI: 10.6084/m9.figshare.14531721.v2
title: RNA splicing programs define tissue compartments and cell types at single cell resolution

description: <b>spliz_*: Separate tables with the SpliZ and SpliZVD score for each cell and gene for each dataset. </b>The cell, gene, cell type, SpliZ, and SpliZVD are given by the cell, geneR1A_uniq, ontology, scZ, and svd_z0 columns respectively. A: human individual 1 10x; B: human individual 2 10x; C: human individual 1 Smart-seq2; D: human individual 2 Smart-seq2; E: lemur individual 1 10x; F: lemur individual 2 10x; G: mouse individual 1 10x; H: mouse individual 2 10x. <br><b>Table 1: Dataset summary.</b> Brief overview of the datasets used in this paper, including tissues seanalyzed, number of cells, median number of spliced reads per cell, sex, and age.<br> <b>Table 2: Differential alternative splicing per compartment. </b>Separate table with the p-value based on the SpliZ and SpliZVD for each gene in each dataset, testing differences between compartments. The gene, SpliZ p-value, SpliZVD p-value, and largest magnitude median for all compartments for SpliZ and SpliZVD for that gene are given by the geneR1A_uniq, perm_pval_adj_scZ, perm_pval_adj_svd_z0, max_abs_median_scZ, and max_abs_median_svd_z0 columns respectively. A: human individual 1 10x; B: human individual 2 10x; C: human individual 1 Smart-seq2; D: human individual 2 Smart-seq2; E: lemur individual 1 10x; F: lemur individual 2 10x; G: mouse individual 1 10x; H: mouse individual 2 10x.<br><br><b>Table 3: Differential alternative splicing per cell type. </b>Separate table with the p-values based on the SpliZ and SpliZVD for each gene in each dataset, testing differences between cell types. The gene, SpliZ p-value, SpliZVD p-value, and largest magnitude median for all compartments for SpliZ and SpliZVD for that gene are given by the geneR1A_uniq, perm_pval_adj_scZ, perm_pval_adj_svd_z0, max_abs_median_scZ, and max_abs_median_svd_z0 columns respectively. A: human individual 1 10x; B: human individual 2 10x; C: human individual 1 Smart-seq2; D: human individual 2 Smart-seq2; E: lemur individual 1 10x; F: lemur individual 2 10x; G: mouse individual 1 10x; H: mouse individual 2 10x.<br><b>Table 4: Most variable splice sites (SpliZsites).</b> The most variable splice sites (SpliZsites) for genes with significant alternative splicing in human individual 1 10x data. Each line reports a SpliZsite and contains the coordinates, whether it is an annotated exon, whether it is an exon with known alternative splicing, whether the splice site is in 5’ or 3’ UTR of the gene, and whether the SpliZsite found to be a SpliZsite in mouse lemur and mouse datasets. <br><br><b>Table 5: Regulated alternative splicing events in spermatogenesis.</b> The list of genes with significantly regulated alternative splicing during sperm development. Each line contains information about the number of cells, spearman's correlation, and its p-value for the human gene and also the same information (based on the mouse and mouse lemur sperm data) for its orthologous genes in mouse and mouse lemur genomes.<br>@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:3 0 0 0 1 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Arial",sans-serif; mso-fareast-font-family:Arial; mso-ansi-language:EN;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:11.0pt; mso-ansi-font-size:11.0pt; mso-bidi-font-size:11.0pt; font-family:"Arial",sans-serif; mso-ascii-font-family:Arial; mso-fareast-font-family:Arial; mso-hansi-font-family:Arial; mso-bidi-font-family:Arial; mso-ansi-language:EN;}.MsoPapDefault {mso-style-type:export-only; line-height:115%;}div.WordSection1 {page:WordSection1;}<br>@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:3 0 0 0 1 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Arial",sans-serif; mso-fareast-font-family:Arial; mso-ansi-language:EN;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:11.0pt; mso-ansi-font-size:11.0pt; mso-bidi-font-size:11.0pt; font-family:"Arial",sans-serif; mso-ascii-font-family:Arial; mso-fareast-font-family:Arial; mso-hansi-font-family:Arial; mso-bidi-font-family:Arial; mso-ansi-language:EN;}.MsoPapDefault {mso-style-type:export-only; line-height:115%;}div.WordSection1 {page:WordSection1;}