DOI: 10.5061/dryad.2p7r5g6
title: Data from: Environmental variables associated with Nothophaeocryptopus gaeumannii population structure and Swiss needle cast severity in Western Oregon and Washington

description: The environment has a strong influence on the abundance and distribution
 of plant pathogenic organisms, and plays a major role in the causation of
 plant disease. Climatological factors may also alter the dynamics of the
 interactions between plant pathogens and their hosts. Nothophaeocryptopus
 (=Phaeocryptopus) gaeumannii, the causal agent of Swiss needle cast (SNC)
 of Douglas-fir, is endemic to western North America where it exists as two
 sympatric, reproductively isolated lineages. The abundance of this fungus
 and the severity of SNC are strongly influenced by climate. We used
 statistical and population genetic analyses to examine relationships
 between environment, pathogen population structure, and SNC severity.
 Although N. gaeumannii Lineage 2 in western Oregon and Washington was most
 abundant where SNC symptoms were most severe, we did not detect a
 significant relationship between Lineage 2 and disease severity. Warmer
 winter temperatures were inversely correlated with foliage retention (AFR)
 and positively correlated with the relative abundance of Lineage 2 (PL2).
 However when distance inland, which was strongly correlated with both AFR
 and PL2, was included in the model, there was no significant relationship
 between Lineage 2 and AFR. Spring/early summer dew point temperatures also
 were positively associated with total N. gaeumannii abundance
 (colonization index (CI)), and inversely correlated with AFR. Warmer
 summer mean temperatures were associated with lower CI and higher AFR. Our
 results suggest that the two lineages have overlapping environmental
 optima, but slightly different tolerance ranges. Lineage 2 was absent from
 more inland sites where winters are colder and summers are warm and dry,
 while Lineage 1 occurred at most sites across an environmental gradient
 suggesting broader environmental tolerance. These relationships suggest
 that climate influences the abundance and distribution of this
 ecologically important plant pathogen, and may have played a role in the
 evolutionary divergence of these two cryptic fungal lineages.
description: Bennett_Nothophaeocryptopus_SSR_PopGen_Data_2014-2016This file includes alleles for 9 microsatellite (SSR) markers for 1,061 isolates of Nothophaeocryptopus gaeumannii collected from Douglas-fir foliage in Oregon and Washington between 2014 and 2016. This file is in GenAlEx format. The isolate name includes, lineage designation (i.e. Lineage 1 or Lineage 2), state where samples were collected, sampling location (i.e. Coos Bay, Tillamook, etc.), site name, tree number, and isolate number separated by underscores. Other column heading correspond to SSR locus names (e.g. Di1, Di2, Di3, etc.).Bennett_SNC_Severity_and_Environmental_Data_OR_WA_2014-2016This file includes SNC severity data (AFR = average foliage retention, CI = colonization index) as well as data for the environmental and geographic variables described in the manuscript. The relative proportions of Nothophaeocryptopus gaeumannii Lineage 1 (PL1) and Lineage 2 (PL2) recovered from each of the sites are also included. These data were used to analyze correlations between Swiss needle cast (SNC) severity, environment, and population structure, and to construct the non-metric multidimensional scaling (NMDS) plot.Bennett_Ecology_and_Evolution_RScript_For_AnalysesThis file includes R script for performing data analyses and creating figures/tables presented in the manuscript. Files, variables, and analyses are described therein.