DOI: 10.5061/dryad.2z34tmpzx
title: Data from: Dopamine and serotonin co-transmission filters striatonigral synaptic activity via 5-HT1B receptor activation

description: The substantia nigra pars reticulata (SNr), a key basal ganglia output
 nucleus, is modulated by dopamine (DA), believed to be released locally
 from midbrain dopamine neurons. Although DA has been proposed to regulate
 GABA release from medium spiny neurons (MSN) terminals via presynaptic D1
 receptors (D1Rs), the precise mechanisms remain unclear. Using presynaptic
 optical recordings of synaptic vesicle fusion, calcium influx in D1-MSN
 synapses, together with postsynaptic patch-clamp recordings from SNr
 neurons, we found that DA inhibits D1-MSN GABA release in a
 frequency-dependent manner. Surprisingly, this effect was independent of
 DA receptors and instead required 5-HT1B receptor activation. Using
 two-photon serotonin biosensor imaging in slices and fiber photometry in
 vivo, we demonstrate that DA enhances extracellular serotonin in the SNr.
 Our results suggest that serotonin mediates DAergic control of basal
 ganglia output and contributes to the therapeutic actions of dopaminergic
 medications for Parkinson’s disease and psychostimulant-related disorders.
description: # Data from: Dopamine and serotonin co-transmission filters striatonigral
 synaptic activity via 5-HT1B receptor activation Dataset DOI:
 [10.5061/dryad.2z34tmpzx](10.5061/dryad.2z34tmpzx) ## Description of the
 data and file structure This dataset contains the source data for all
 figures. Each CSV file corresponds to one figure panel as indicated by the
 filename. \- Columns: "x" = time or condition "y" =
 measurement (e.g., normalized fluorescence, ΔF/F, etc.) "sem" =
 standard error of the mean (if applicable) "n" = number of
 observations (if applicable) For details on experimental design, see
 Materials and Methods in the manuscript. Contact: Anders Borgkvist,
 Department of Neuroscience, Karolinska Institutet,
 [anders.borgkvist@ki.se](mailto:anders.borgkvist@ki.se) ### Files and
 variables #### File: Dataset_Molinari_etal.zip **Root Contents** •
 **README.txt** — Text note/README. **Fiber_photometry_analysis_code** •
 **README_FP.txt** – Type: Text file • **ann_5HT_grab.m** – Type: M
 **Figure1** • **Fig1C_source.csv** – Type: CSV (comma-delimited) – Shape:
 198 rows × 5 columns – Variables: Time_s, Condition, Mean, SEM, N – Notes:
 Time-course dataset with condition averages and SEM. •
 **Fig1D_source.csv** – Type: CSV (comma-delimited) – Shape: 39 rows × 6
 columns – Variables: RowType, Condition, Mean, SEM, N, FractionalRelease –
 Notes: Summary/condition table with mean, SEM, N. • **Fig1E_source.csv** –
 Type: CSV (comma-delimited) – Shape: 39 rows × 6 columns – Variables:
 RowType, Condition, Mean, SEM, N, BoutonsPerSlice – Notes:
 Summary/condition table with mean, SEM, N. • **Fig1F_source.csv** – Type:
 CSV (comma-delimited) – Shape: 198 rows × 5 columns – Variables: Time_s,
 Condition, Mean, SEM, N – Notes: Time-course dataset with condition
 averages and SEM. • **Fig1G_source.csv** – Type: CSV (comma-delimited) –
 Shape: 12 rows × 6 columns – Variables: RowType, Condition, Mean, SEM, N,
 FractionalRelease – Notes: Summary/condition table with mean, SEM, N.
 **Figure2** • **Fig2A_source.csv** – Type: CSV (comma-delimited) – Shape:
 264 rows × 5 columns – Variables: Time_s, Condition, Mean, SEM, N – Notes:
 Time-course dataset with condition averages and SEM. •
 **Fig2B_source.csv** – Type: CSV (comma-delimited) – Shape: 39 rows × 6
 columns – Variables: RowType, Condition, Mean, SEM, N, FractionalRelease –
 Notes: Summary/condition table with mean, SEM, N. • **Fig2C_source.csv** –
 Type: CSV (comma-delimited) – Shape: 198 rows × 5 columns – Variables:
 Time_s, Condition, Mean, SEM, N – Notes: Time-course dataset with
 condition averages and SEM. • **Fig2D_source.csv** – Type: CSV
 (comma-delimited) – Shape: 26 rows × 6 columns – Variables: RowType,
 Condition, Mean, SEM, N, FractionalRelease – Notes: Summary/condition
 table with mean, SEM, N. • **Fig2F_source.csv** – Type: CSV
 (comma-delimited) – Shape: 41 rows × 6 columns – Variables: RowType,
 Condition, Mean, SEM, N, FractionalRelease – Notes: Summary/condition
 table with mean, SEM, N. **Figure3** • **Fig3C_source.csv** – Type: CSV
 (comma-delimited) – Shape: 278 rows × 4 columns – Variables: avgDA, semDA,
 avgCtr, semCtr – Notes: Tabular dataset. • **Fig3D_source.csv** – Type:
 CSV (comma-delimited) – Shape: 4 rows × 4 columns – Variables: diffDA,
 diffSKF, semSKF, semDA – Notes: Tabular dataset. **Figure4** •
 **Fig4G_source.csv** – Type: CSV (semicolon-delimited) – Shape: 50 rows ×
 5 columns – Variables: Time_m, Condition, Mean, SEM, N – Notes:
 Time-course dataset with condition averages and SEM. •
 **Fig4H_source.csv** – Type: CSV (semicolon-delimited) – Shape: 40 rows ×
 6 columns – Variables: RowType, Condition, Mean, SEM, N, oIPSC – Notes:
 Summary/condition table with mean, SEM, N. • **Fig4I_source.csv** – Type:
 CSV (semicolon-delimited) – Shape: 35 rows × 8 columns – Variables:
 RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7 –
 Notes: Summary/condition table with mean, SEM, N. • **Fig4K_source.csv** –
 Type: CSV (semicolon-delimited) – Shape: 17 rows × 9 columns – Variables:
 RowType, Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed:
 8 – Notes: Summary/condition table with mean, SEM, N. **Figure5** •
 **Fig5C_source .csv** – Type: CSV (semicolon-delimited) – Shape: 30 rows ×
 5 columns – Variables: Time_m, Condition, Mean, SEM, N – Notes:
 Time-course dataset with condition averages and SEM. •
 **Fig5D_source.csv** – Type: CSV (semicolon-delimited) – Shape: 38 rows ×
 12 columns – Variables: RowType, Condition, Mean, SEM, N, oIPSC, Unnamed:
 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11 – Notes:
 Summary/condition table with mean, SEM, N. • **Fig5E_source.csv** – Type:
 CSV (semicolon-delimited) – Shape: 30 rows × 5 columns – Variables:
 Time_m, Condition, Mean, SEM, N – Notes: Time-course dataset with
 condition averages and SEM. • **Fig5F_source.csv** – Type: CSV
 (semicolon-delimited) – Shape: 37 rows × 12 columns – Variables: RowType,
 Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8,
 Unnamed: 9, Unnamed: 10, Unnamed: 11 – Notes: Summary/condition table with
 mean, SEM, N. • **Fig5G_source.csv** – Type: CSV (comma-delimited) –
 Shape: 278 rows × 6 columns – Variables: avgDA, semDA, avgCtr, semCtr,
 avgNAS_DA, semNAS_DA – Notes: Tabular dataset. • **Fig5H_source.csv** –
 Type: CSV (comma-delimited) – Shape: 4 rows × 5 columns – Variables:
 semNAS_DA, semDA, diffNAS_DA, diffDA, freq – Notes: Tabular dataset.
 **Figure6** • **Fig6C_source.csv** – Type: CSV (comma-delimited) – Shape:
 600 rows × 9 columns – Variables: xVal, avg1micro, sem1micro, xVal.1,
 mean_sec, sem_sec, xVal.2, avg100nM, sem100nM – Notes: Tabular dataset. •
 **Fig6D_source.csv** – Type: CSV (comma-delimited) – Shape: 35 rows × 6
 columns – Variables: RowType, Condition, Mean, SEM, N,
 g5HT3_DeltaF_over_F_percent – Notes: Summary/condition table with mean,
 SEM, N. • **Fig6E_source.csv** – Type: CSV (comma-delimited) – Shape: 16
 rows × 6 columns – Variables: RowType, Condition, Mean, SEM, N,
 g5HT3_DeltaF_over_F_percent – Notes: Summary/condition table with mean,
 SEM, N. • **Fig6F_source.csv** – Type: CSV (comma-delimited) – Shape: 14
 rows × 9 columns – Variables: RowType, Condition, Value, Value_display,
 Mean, SEM, N, Mean_display, SEM_display – Notes: Summary/condition table
 with mean, SEM, N. **Figure7** • **Fig7A_source.csv** – Type: CSV
 (semicolon-delimited) – Shape: 21 rows × 9 columns – Variables: time
 (min), DA 10 µM, sem_DA, time (min).1, Cit 10 µM, sem_Cit, time (min).2,
 Cit+DA, sem_Cit+DA – Notes: Tabular dataset. • **Fig7B_source.csv** –
 Type: CSV (comma-delimited) – Shape: 49 rows × 6 columns – Variables:
 RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent – Notes:
 Summary/condition table with mean, SEM, N. • **Fig7D_source.csv** – Type:
 CSV (comma-delimited) – Shape: 162 rows × 8 columns – Variables:
 DA10_t_avg, DA10_t_sem, DA50_t_avg, DA50_t_sem, aCSF_t_avg, aCSF_t_sem,
 TBZ_t_avg, TBZ_t_sem – Notes: Tabular dataset. • **Fig7E_source.csv** –
 Type: CSV (comma-delimited) – Shape: 24 rows × 5 columns – Variables:
 Time_min, Condition, Mean, SEM, N – Notes: Tabular dataset. •
 **Fig7F_source.csv** – Type: CSV (comma-delimited) – Shape: 21 rows × 6
 columns – Variables: RowType, Condition, Mean, SEM, N,
 g5HT3_DeltaF_over_F_percent – Notes: Summary/condition table with mean,
 SEM, N. **Figure8** • **Fig8B_source.csv** – Type: CSV (comma-delimited) –
 Shape: 21 rows × 6 columns – Variables: RowType, Condition, Mean, SEM, N,
 g5HT3_DeltaF_over_F_percent – Notes: Summary/condition table with mean,
 SEM, N. • **Fig8D_source.csv** – Type: CSV (semicolon-delimited) – Shape:
 42 rows × 12 columns – Variables: RowType, Condition, Mean, SEM, N,
 light-evoked eIPSC change (%), Unnamed: 6, Unnamed: 7, Unnamed: 8,
 Unnamed: 9, Unnamed: 10, Unnamed: 11 – Notes: Summary/condition table with
 mean, SEM, N. **Figure9** • **Fig9C_source .csv** – Type: CSV
 (semicolon-delimited) – Shape: 37 rows × 12 columns – Variables: RowType,
 Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8,
 Unnamed: 9, Unnamed: 10, Unnamed: 11 – Notes: Summary/condition table with
 mean, SEM, N. • **Fig9D_source.csv** – Type: CSV (semicolon-delimited) –
 Shape: 37 rows × 13 columns – Variables: RowType, Condition, Mean, SEM, N,
 oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10,
 Unnamed: 11, Unnamed: 12 – Notes: Summary/condition table with mean, SEM,
 N. • **Fig9E_source.csv** – Type: CSV (semicolon-delimited) – Shape: 37
 rows × 13 columns – Variables: RowType, Condition, Mean, SEM, N, PPR
 value, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10,
 Unnamed: 11, Unnamed: 12 – Notes: Summary/condition table with mean, SEM,
 N. • **Fig9G_source.csv** – Type: CSV (comma-delimited) – Shape: 276 rows
 × 4 columns – Variables: ctr_sem, avg_ctr, avg_cp, cp_sem – Notes: Tabular
 dataset. • **Fig9H_source.csv** – Type: CSV (comma-delimited) – Shape: 4
 rows × 3 columns – Variables: freq, FluoDecr, FluoDecrSem – Notes: Tabular
 dataset. **Figure10** • **Fig10C_source.csv** – Type: CSV
 (semicolon-delimited) – Shape: 433330 rows × 18 columns – Variables: Time
 (mins), Vehicle, Unnamed: 2, Unnamed: 3, Unnamed: 4, Unnamed: 5, Unnamed:
 6, GBR, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11, Unnamed: 12,
 Citalopram, Unnamed: 14, Unnamed: 15, Unnamed: 16, Unnamed: 17 – Notes:
 Tabular dataset. • **Fig10D_source.csv** – Type: CSV (semicolon-delimited)
 – Shape: 37 rows × 13 columns – Variables: RowType, Condition, Mean, SEM,
 N, zF, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10,
 Unnamed: 11, Unnamed: 12 – Notes: Summary/condition table with mean, SEM,
 N. **FigureS1** • **FigS1A_source.csv** – Type: CSV (comma-delimited) –
 Shape: 132 rows × 5 columns – Variables: Time_s, Condition, Mean, SEM, N –
 Notes: Time-course dataset with condition averages and SEM. •
 **FigS1B_source.csv** – Type: CSV (comma-delimited) – Shape: 21 rows × 6
 columns – Variables: RowType, Condition, Mean, SEM, N, FractionalRelease –
 Notes: Summary/condition table with mean, SEM, N. • **FigS1C_source.csv**
 – Type: CSV (comma-delimited) – Shape: 18 rows × 6 columns – Variables:
 RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent – Notes:
 Summary/condition table with mean, SEM, N. **FigureS2** • **FigS2B.csv** –
 Type: CSV (comma-delimited) – Shape: 59 rows × 5 columns – Variables:
 Time_s, Condition, Mean, SEM, N – Notes: Time-course dataset with
 condition averages and SEM. • **FigS2C.csv** – Type: CSV (comma-delimited)
 – Shape: 16 rows × 7 columns – Variables: RowType, Condition, Mean, SEM,
 N, FiringFrequency_Hz, PairID – Notes: Summary/condition table with mean,
 SEM, N. • **FigS2D.csv** – Type: CSV (comma-delimited) – Shape: 132 rows ×
 5 columns – Variables: Time_s, Condition, Mean, SEM, N – Notes:
 Time-course dataset with condition averages and SEM. • **FigS2E.csv** –
 Type: CSV (comma-delimited) – Shape: 132 rows × 5 columns – Variables:
 Time_s, Condition, Mean, SEM, N – Notes: Time-course dataset with
 condition averages and SEM. • **FigS2F.csv** – Type: CSV (comma-delimited)
 – Shape: 132 rows × 5 columns – Variables: Time_s, Condition, Mean, SEM, N
 – Notes: Time-course dataset with condition averages and SEM. •
 **FigS2G.csv** – Type: CSV (comma-delimited) – Shape: 132 rows × 5 columns
 – Variables: Time_s, Condition, Mean, SEM, N – Notes: Time-course dataset
 with condition averages and SEM. • **FigS2H.csv** – Type: CSV
 (comma-delimited) – Shape: 10 rows × 6 columns – Variables: RowType,
 Condition, Mean, SEM, N, FractionalRelease – Notes: Summary/condition
 table with mean, SEM, N. • **FigS2I.csv** – Type: CSV (comma-delimited) –
 Shape: 10 rows × 6 columns – Variables: RowType, Condition, Mean, SEM, N,
 FractionalRelease – Notes: Summary/condition table with mean, SEM, N. •
 **FigS2J.csv** – Type: CSV (comma-delimited) – Shape: 12 rows × 6 columns
 – Variables: RowType, Condition, Mean, SEM, N, FractionalRelease – Notes:
 Summary/condition table with mean, SEM, N. • **FigS2K.csv** – Type: CSV
 (comma-delimited) – Shape: 18 rows × 6 columns – Variables: RowType,
 Condition, Mean, SEM, N, FractionalRelease – Notes: Summary/condition
 table with mean, SEM, N. **FigureS3** • **FigS3B.csv** – Type: CSV
 (comma-delimited) – Shape: 32 rows × 6 columns – Variables: RowType,
 Condition, Mean, SEM, N, GRAB_DA_dFF_percent_of_baseline – Notes:
 Summary/condition table with mean, SEM, N. • **FigS3E.csv** – Type: CSV
 (comma-delimited) – Shape: 161 rows × 6 columns – Variables: avg_nostim,
 sem_nostim, avg_2hz, sem_2hz, avg_10hz, sem_10hz – Notes: Tabular dataset.
 • **FigS3F.csv** – Type: CSV (comma-delimited) – Shape: 31 rows × 6
 columns – Variables: RowType, Condition, Mean, SEM, N,
 GRAB_DA_dFF_percent_of_baseline – Notes: Summary/condition table with
 mean, SEM, N. • **FigS3G.csv** – Type: CSV (comma-delimited) – Shape: 161
 rows × 6 columns – Variables: avg_nostim, sem_nostim, avg_2hz, sem_2hz,
 avg_10hz, sem_10hz – Notes: Tabular dataset. • **FigS3H.csv** – Type: CSV
 (comma-delimited) – Shape: 24 rows × 6 columns – Variables: RowType,
 Condition, Mean, SEM, N, GRAB_DA_dFF_percent_of_baseline – Notes:
 Summary/condition table with mean, SEM, N. **FigureS4** •
 **FigS4A_source.csv** – Type: CSV (semicolon-delimited) – Shape: 29 rows ×
 9 columns – Variables: RowType, Condition, Mean, SEM, N, PPR value,
 Unnamed: 6, Unnamed: 7, Unnamed: 8 – Notes: Summary/condition table with
 mean, SEM, N. • **FigS4B_source.csv** – Type: CSV (semicolon-delimited) –
 Shape: 29 rows × 10 columns – Variables: RowType, Condition, Mean, SEM, N,
 PPR value, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9 – Notes:
 Summary/condition table with mean, SEM, N. • **FigS4C_source.csv** – Type:
 CSV (semicolon-delimited) – Shape: 31 rows × 9 columns – Variables:
 RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7,
 Unnamed: 8 – Notes: Summary/condition table with mean, SEM, N. •
 **FigS4D_source.csv** – Type: CSV (semicolon-delimited) – Shape: 29 rows ×
 9 columns – Variables: RowType, Condition, Mean, SEM, N, PPR value,
 Unnamed: 6, Unnamed: 7, Unnamed: 8 – Notes: Summary/condition table with
 mean, SEM, N.