DOI: 10.5281/zenodo.11113844
title: Selective autophagy acts as an immune rheostat to balance  plant defense with cellular survival during viral infection - part I

description: The dataset contains all the original raw files for the main figures to the following study  - and is a second version of the original preprinted work.

Marion Clavel1,2*, Anita Bianchi1,3, Roksolana Kobylinska1, Roan Groh1,4, Xi Zhang2, Juncai Ma5, Ranjith K. Papareddy1,3, Nenad Grujic1, Lorenzo Picchianti1,4, Ethan Stewart5, Michael Schutzbier1, Karel Stejskal1, Juan Carlos de la Concepcion1, Victor Sanchez de Medina Hernandez1,4,8, Yoav Voichek1,9, Pieter Clauw1, Joanna Gunis1, Gerhard Durnberger1, Jens Christian Muelders2, Annett Grimm2, Arthur Sedivy6,10, Mathieu Erhardt7, Victoria Vyboishchikov1, Peng Gao1, Esther Lechner7, Emilie Vantard7, Thomas Potuschak7, Jakub Jez6,11, Elisabeth Roitinger1, Pascal Genschik7, Byung-Ho Kang5, Yasin Dagdas1,3*

Affiliations:                                                                                                                 

1Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter; Vienna, Austria.

2Max-Planck-Institut für Molekulare Pflanzenphysiologie; Potsdam-Golm, Germany.

3Heidelberg University, Centre for Organismal Studies (COS); 69120 Heidelberg, Germany. 

4Vienna BioCenter PhD Program, Doctoral School of the University at Vienna and Medical University of Vienna; Vienna, Austria.

5School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories; Hong Kong, China.

6Vienna Biocenter Core Facilities (VBCF); Vienna, Austria.

7 Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg; 12, rue du Général Zimmer, 67084 Strasbourg, France.

8Current affiliation: Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland

9Current affiliation: Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel

10Current affiliation: BOKU University, Core Facility Biomolecular & Cellular Analysis, Vienna, Austria

11Current affiliation: Australian Institute of Marine Science; PMB 3, Townsville MC, QLD, 4810, Australia

*Corresponding authors. Email: Marion Clavel (marion.clavel@mpimp-golm.mpg.de), Yasin Dagdas (yasin.dagdas@cos.uni-heidelberg.de)

 

Abstract: RNA viruses co-opt host endomembranes to form replication complexes, often triggering cellular stress and immune responses. Here, we show that Arabidopsis thaliana activates selective autophagy to respond to viruses targeting mitochondria, chloroplasts, and the endoplasmic reticulum. Rather than degrading viral components, autophagy selectively removes the immune regulator Enhanced Disease Susceptibility 1 (EDS1) to prevent cell death. This targeted mechanism is mediated by oligomeric metabolic enzymes that moonlight as selective autophagy receptors, linking organelle stress to immune homeostasis. Our findings establish selective autophagy as an essential immune rheostat, fine-tuning defense responses and safeguarding cellular integrity to promote host survival during viral infections.

 

One archive corresponds to one main figure.