DOI: 10.5061/dryad.q2bvq83w0
title: Sequencing results of the gut microbiota in zebrafish after the use of anti-Vibrio vulnificus immunoglobulin Y (IgY) antibodies

description: This study investigates the protective effects of immunoglobulin Y (IgY)
 antibodies as a targeted treatment against Vibrio vulnificus infection in
 zebrafish (Danio rerio), focusing on survival outcomes and microbiota
 modulation. Zebrafish were administered IgY antibodies through injection
 and oral routes prior to V. vulnificus exposure. Results showed that IgY
 treatment significantly enhanced survival rates, with injected groups
 achieving up to 90% survival compared to only 40% in untreated controls.
 This protective effect is attributed to IgY pathogen-specific action
 against V. vulnificus, limiting bacterial load and infection severity.
 Additionally, 16S rRNA sequencing revealed favorable shifts in gut
 microbiota among IgY-treated fish, with increased abundances of beneficial
 bacteria such as Lactobacillus and Faecalibacterium prausnitzii, alongside
 reduced levels of pathogenic genera like Vibrio and Aeromonas. These
 findings highlight IgY dual benefits: pathogen-specific inhibition and
 microbiota-friendly action, positioning it as a promising alternative to
 antibiotics in aquaculture. IgY targeted approach supports microbial
 health and reduces risks of antibiotic resistance, aligning with
 sustainable aquaculture goals. Future research should explore optimized
 IgY dosing and its effects across different fish species, positioning IgY
 as a valuable tool for antibiotic-free disease management in fish farming.
description: # Sequencing results of the gut microbiota in zebrafish after the use of
 anti-Vibrio vulnificus immunoglobulin Y (IgY) antibodies
 [https://doi.org/10.5061/dryad.q2bvq83w0](https://doi.org/10.5061/dryad.q2bvq83w0) ## Description of the data and file structure Bacteria_A1_raw.fq.gz、Bacteria_A2_raw.fq.gz、Bacteria_A3_raw.fq.gz : Model groups (A1, A2, A3 Three replicates.): Adult zebrafish weighing 0.2 g were injected with 10 μL of Vibrio vulnificus resuspended in PBS solution (approximately 4×10^6 CFU/mL). Sequencing the intestinal microbiota of zebrafish. Bacteria_B1_raw.fq.gz、Bacteria_B2_raw.fq.gz、Bacteria_B3_raw.fq.gz : Blank control group(B1, B2, B3 Three replicates.) ：Adult zebrafish weighing 0.2 g received no treatment. Sequencing the intestinal microbiota of zebrafish. Bacteria_C1_raw.fq.gz、Bacteria_C2_raw.fq.gz、Bacteria_C3_raw.fq.gz : IgY injection group（C1、C2、C3 Three replicates.）: Adult zebrafish weighing 0.2 g were injected with only 10 μL of anti-Vibrio vulnificus egg yolk antibody (IgY). IgY injection group: Adult zebrafish weighing 0.2 g were injected with only 10 μL of anti-Vibrio vulnificus egg yolk antibody (IgY). Sequencing the intestinal microbiota of zebrafish. Bacteria_D1_raw.fq.gz、Bacteria_D2_raw.fq.gz、Bacteria_D3_raw.fq.gz : IgY prevention groups (D1, D2, D3 Three replicates.): Adult zebrafish weighing 0.2 g were first injected with 10 μL of anti-Vibrio vulnificus egg yolk antibody (IgY) and, after 1 hour, were injected with 10 μL of Vibrio vulnificus resuspended in PBS solution (approximately 4×10^6 CFU/mL). Sequencing the intestinal microbiota of zebrafish.