DOI: 10.5061/dryad.0p2ngf27k
title: Data from: The ectomycorrhizal fungus Paxillus ammoniavirescens influences the effects of salinity on loblolly pine in response to potassium availability

description: Salinity is an increasing problem in coastal areas affected by saltwater
 intrusion, with deleterious effects on tree health and forest growth.
 Ectomycorrhizal (ECM) fungi may improve salinity tolerance of host trees,
 but the impact of external potassium (K+) availability on these effects is
 still unclear. Here, we performed several experiments with the ECM fungus
 Paxillus ammoniavirescens and loblolly pine (Pinus taeda L.) in
 axenic and symbiotic conditions at limited or sufficient K+ and increasing
 sodium (Na+) concentrations. Growth rate, biomass, nutrient content, and
 K+ transporter expression levels were recorded for the fungus, and the
 colonization rate, root development parameters, biomass, and shoot
 nutrient accumulation were determined for mycorrhizal and non-mycorrhizal
 plants. P. ammoniavirescens was tolerant to high salinity, although growth
 and nutrient concentrations varied with K+ availability and increasing Na+
 exposure. While loblolly pine root growth and development decreased with
 increasing salinity, ECM colonization was unaffected by pine response to
 salinity. The mycorrhizal influence on loblolly pine salinity response was
 strongly dependent on external K+ availability. This study reveals that P.
 ammoniavirescens can reduce Na+ accumulation of salt-exposed loblolly
 pine, but this effect depends on external K+ availability.
description: # Data from: The ectomycorrhizal fungus Paxillus ammoniavirescens
 influences the effects of salinity on loblolly pine in response to
 potassium availability 
 Dataset contains all biomass, nutrient concentrations, fungal and plant
 growth parameters, Ct, and colonization data obtained from the experiments
 described in the manuscript. ## **Description of the data and file
 structure** Dataset are presented in multiple spreadsheets that
 corresponds to the different experiments performed in the corresponding
 manuscript. Missing values or not collected data are labeled n/a. Below is
 a breakdown of each experiment and technical details. ####
 **fungalBiomass:** axenic experiment in liquid culture to determine
 biomass and nutrient concentrations. * SampleID: sample identification
 number * K_mM: potassium treatment * Na_mM: sodium treatment * biomass_g:
 fungal biomass * K_mg/g: fungal potassium concentrations * Na_mg/g: fungal
 sodium concentrations * K:Na: calculation of potassium/sodium ratios ####
 **fungalqPCRCts:** Ct from qPCR on targeted genes from axenic experiment
 in liquid culture. * SampleID: sample identification number * K_mM:
 potassium treatment * Na_mM: sodium treatment * Ct-PaTub: Ct value for
 *PaTub* * Ct-PaTrk: Ct value for *PaTrk* * Ct-PaACU1: Ct value for
 *PaACU1* * Ct-PaACU2: Ct value for *PaACU2* #### **fungalRadialGrowth:**
 axenic experiment on solid plate to determine radial growth. Each value
 “10” represents 10 mm (i.e. the diameter of the original plug), meaning no
 growth was recorded. * SampleID: sample identification number * day: days
 after the experiment started * K_mM: potassium treatment * Na_mM: sodium
 treatment  * mm1: first diameter measurement * mm2: second diameter
 measurement  * mm3: third diameter measurement      * mmAvg: average
 measurement from mm1, mm2 and mm3 * radAvg: average radius * mm_day:
 growth per day in millimeter #### **coCulture4WkRoot:** co-culture
 experiment to determine the effect of salinity on ectomycorrhiza formation
 and root development (4 weeks). * SampleID: sample identification number *
 K_mM: potassium treatment * Na_mM: sodium treatment * root_dw: root dry
 weight (g) * col_nm_sr: number of non-mycorrhizal short root * col_m_sr :
 number of mycorrhizal short root * col_sr_total: total number of short
 root * col_p: percentage of colonization * primary_rt_mm: primary root
 length in millimeter * lat_rt_mm: average lateral root length in
 millimeter * total_lateral_rts: total number of lateral roots *
 total_rt_mm: total lateral root length in millimeter ####
 **coCulture8WkNaK:**  co-culture experiment to determine the effect of
 symbiosis on plant salinity tolerance (8 weeks). * Sample_ID: sample
 identification number * Rep: replicate (same number means same plant) *
 plantPart: plant tissue (shoot or root) * K_mM: potassium treatment *
 Na_mM: sodium treatment * ECM: mycorrhizal status * fw_g: fresh weight *
 dw_g: dry weight * col%: percentage of colonization * K_mg_g: shoot
 potassium concentrations * Na_mg_g: shoot sodium concentrations * K:Na:
 calculation of potassium/sodium ratios #### **coCulture8WqPCRCts:** Ct
 from qPCR on targeted genes from co-culture experiment (8 weeks). *
 SampleID: sample identification number * K_mM: potassium treatment *
 Na_mM: sodium treatment * Ct-PaTub: Ct value for *PaTub* * Ct-PaTrk: Ct
 value for *PaTrk* * Ct-PaACU1: Ct value for *PaACU1* * Ct-PaACU2: Ct value
 for *PaACU2*