DOI: 10.5061/dryad.qm0b25h
title: Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance

description: Disruption of neuronal function is likely to influence limits to thermal
 tolerance. We hypothesized that with acute warming the structure and
 function of neuronal membranes in the Antarctic notothenioid fish
 Chaenocephalus aceratus are more vulnerable to perturbation than membranes
 in the more thermotolerant notothenioid Notothenia coriiceps. Fluidity was
 quantified in synaptic membranes, mitochondrial membranes, and myelin from
 brains of both species of Antarctic fishes. Polar lipid compositions and
 cholesterol contents were analyzed in myelin; cholesterol was measured in
 synaptic membranes. Thermal profiles were determined for activities of two
 membrane-associated proteins, acetylcholinesterase (AChE) and
 Na+/K+-ATPase (NKA), from brains of animals maintained at ambient
 temperature or exposed to their critical thermal maxima (CTMAX). Synaptic
 membranes of C. aceratus were consistently more fluid than those of N.
 coriiceps (P &lt; 0.0001). Although the fluidities of both myelin and
 mitochondrial membranes were similar among species, sensitivity of myelin
 fluidity to in vitro warming was greater in N. coriiceps than in C.
 aceratus (P &lt; 0.001), which can be explained by lower cholesterol
 contents in myelin of N. coriiceps (P &lt; 0.05). Activities of both
 enzymes, AChE and NKA, declined upon CTMAX exposure in C. aceratus, but
 not in N. coriiceps. We suggest that hyper-fluidization of synaptic
 membranes with warming in C. aceratus may explain the greater stenothermy
 in this species, and that thermal limits in notothenioids are more likely
 to be influenced by perturbations in synaptic membranes than in other
 membranes of the nervous system.
description: Biederman et al (2019)
 AChE activityAcetylcholine esterase
 activity in brains of Chaenocephalus aceratus and Notothenia
 coriiceps.Biederman et al (2019)
 cholesterolCholeresterol in myelin
 and synaptic membranes from brains of Chaenocephalus aceratus and
 Notothenia coriiceps.Biederman et al (2019)
 LDH activityLactate dehydrogenase
 activity in brains of Chaenocephalus aceratus and Notothenia
 coriiceps.Biederman et al (2019)
 marker enzyme enrichmentsMarker enzymes for
 neuronal membrane preparations in Chaenocephalus aceratus and Notothenia
 coriiceps.Biederman et al (2019)
 mitochondrial fluidityFluidity of
 mitochondrial membranes in brains of Chaenocephalus aceratus and
 Notothenia coriiceps.Biederman et al (2019)
 myelin fluidityFluidity of myelin in
 brains of Chaenocephalus aceratus and Notothenia
 coriiceps.Biederman et al (2019)
 NKA activitySodium-potassium ATPase
 activity in brains of Notothenia coriiceps and Chaenocephalus
 aceratus.Biederman et al (2019)
 PK activityPyruvate kinase activity
 in brains of Notothenia coriiceps and Chaenocephalus
 aceratus.Biederman et al (2019)
 polar lipid profilePolar lipid composition
 of myelin in Notothenia coriiceps and Chaenocephalus
 aceratusBiederman et al (2019)
 synaptic membrane fluidityFluidity of synaptic
 membranes in brains of Notothenia coriiceps and Chaenocephalus
 aceratus.Biederman et al (2019)
 KmKm
 of acetylcholine esterase (for acetylthiocholine iodide) in Notothenia
 coriiceps and Chaenocephalus aceratus.