DOI: 10.5061/dryad.sj3tx969t
title: Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning

description: O-GlcNAcylation is a dynamic post-translational modification that
 diversifies the proteome. Its dysregulation is associated with
 neurological disorders that impair cognitive function, and yet
 identification of phenotype-relevant candidate substrates in a
 brain-region-specific manner remains unfeasible. By combining an O-GlcNAc
 binding activity derived from Clostridium
 perfringens OGA (CpOGA) with TurboID proximity labeling in
 Drosophila, we developed an O-GlcNAcylation profiling tool that translates
 O-GlcNAc modification into biotin conjugation for tissue-specific
 candidate substrates enrichment. We mapped the O-GlcNAc interactome in
 major brain regions of Drosophila and found that components of
 the translational machinery, particularly ribosomal subunits, were
 abundantly O-GlcNAcylated in the mushroom body of
 Drosophila brain. Hypo-O-GlcNAcylation induced by ectopic
 expression of active CpOGA in the mushroom body decreased local
 translational activity, leading to olfactory learning deficits that could
 be rescued by dMyc overexpression-induced increase of protein synthesis.
 Our study provides a useful tool for future dissection of tissue-specific
 functions of O-GlcNAcylation in Drosophila and suggests a
 possibility that O-GlcNAcylation impacts cognitive function via regulating
 regional translational activity in the brain.
description: # Data from: Tissue-specific O-GlcNAcylation profiling identifies
 substrates in translational machinery in the *Drosophila* mushroom body
 contributing to olfactory learning DOI:[10.5061/dryad.sj3tx969t
 ](https://doi.org/10.5061/dryad.sj3tx969t) This dataset includes the data
 in the above publication. In this publication, we developed an
 *O*-GlcNAcylation profiling tool that translates *O*-GlcNAc modification
 into biotin conjugation for tissue-specific candidate substrate
 enrichment. We mapped the *O*-GlcNAc interactome in major brain regions of
 *Drosophila* and found that components of the translational machinery,
 particularly ribosomal subunits, were abundantly *O*-GlcNAcylated in the
 mushroom body of *Drosophila* brain. Hypo-*O*-GlcNAcylation induced by
 ectopic expression of active *Cp*OGA in the mushroom body decreased local
 translational activity, leading to olfactory learning deficits that could
 be rescued by dMyc overexpression-induced increase of protein synthesis. #
 Description of the data and file structure  Files: 1\. Figure source data:
 This file contains the original files and data for all the figures in the
 article (This includes raw data from Western blot, immunofluorescence,
 QPCR, statistical graphs, behavioral experiments, GO analysis, and more).
 2\. Supplementary file: This file contains 11 supplementary files in the
 article. # Sharing/Access information: 1\. The accession numbers for the
 mass spectrometry data were PXD040547 and PXD040412 on the ProteomeXchange
 Consortium PRIDE partner repository. 2\. We created an online database for
 tissue-specific O-GlcNAcylation Atlas of Drosophila Brain (tsOGA, link in
 Related Works) to host these datasets.