cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19944763.v1
title: STABILITY OF ANTHOCYANINS FROM AGROINDUSTRIAL RESIDUE OF ISABEL GRAPE GROWN IN SÃO FRANCISCO VALLEY, BRAZIL

description: ABSTRACT Grape by-products are promising and sustainable sources of anthocyanins. Considering the factors related to degradation of those pigments, spectrophotometric and kinetic analyzes were carried out with the aim to evaluate the light and heat stability of anthocyanins from Isabel grape residue. We found that the exposure to luminosity (80W; 2500 lux) and high temperature (= 70ºC) significantly affected the absorption and content of those compounds (p &lt;0.05), whose the most stable condition was obtained at 50°C (t1/2: 37.7h; k: 1.84 x 10-2 h-1) and protected from light (t1/2: 3,320.6h; k: 2.09 x 10-4 h-1). Therefore, the anthocyanins from the agroindustrial residue of Isabel grape are an alternative of natural colorants for food that can be packaged in opaque packaging and not subjected to severe heat treatment during processing.
DOI: 10.6084/m9.figshare.19944763
title: STABILITY OF ANTHOCYANINS FROM AGROINDUSTRIAL RESIDUE OF ISABEL GRAPE GROWN IN SÃO FRANCISCO VALLEY, BRAZIL

description: ABSTRACT Grape by-products are promising and sustainable sources of anthocyanins. Considering the factors related to degradation of those pigments, spectrophotometric and kinetic analyzes were carried out with the aim to evaluate the light and heat stability of anthocyanins from Isabel grape residue. We found that the exposure to luminosity (80W; 2500 lux) and high temperature (= 70ºC) significantly affected the absorption and content of those compounds (p &lt;0.05), whose the most stable condition was obtained at 50°C (t1/2: 37.7h; k: 1.84 x 10-2 h-1) and protected from light (t1/2: 3,320.6h; k: 2.09 x 10-4 h-1). Therefore, the anthocyanins from the agroindustrial residue of Isabel grape are an alternative of natural colorants for food that can be packaged in opaque packaging and not subjected to severe heat treatment during processing.
DOI: 10.6084/m9.figshare.19944764.v1
title: ‘FUJI’ APPLE TREE RESPONSE TO PHOSPHORUS FERTILIZATION

description: ABSTRACT The aim of this work was to evaluate the effects of orchard fertilization with increasing rates of phosphorus (P) on yield and critical levels of P in the soil and in the leaf of ‘Fuji’ apple trees. The experiment was conducted in São Joaquim, state of Santa Catarina, southern Brazil, from 2010 to 2015, in an Inceptisol soil. The three apple orchards were planted in 2004 with the Fuji cultivar grafted on Marubakaido/ M9 rootstock and grown under a high-plant-density system (1984 trees ha-1). Annual fertilizer levels of 0, 40, 80, 120 and 160 kg ha-1 of P2O5 (as triple superphosphate) were applied to the soil surface, without incorporation, in a randomized block design with five replicates. In the first and last years (2011 and 2015), soil samples were collected from 0-10, 10-20 and 0-20 cm layers and the available P content was analyzed. Annually, whole leaves were collected and analyzed for P content. The number and weight of fruits per tree and hfruit yield were also evaluated. Application of P increased the content of available P in the soil, but this increase was not accompanied by increased leaf P content nor by increased fruit yield. This suggests that, in soils with medium to high content of organic matter and well fertilized with P before planting, there is no response of apple trees for P reapplication in the 10 subsequent years.
DOI: 10.6084/m9.figshare.19944764
title: ‘FUJI’ APPLE TREE RESPONSE TO PHOSPHORUS FERTILIZATION

description: ABSTRACT The aim of this work was to evaluate the effects of orchard fertilization with increasing rates of phosphorus (P) on yield and critical levels of P in the soil and in the leaf of ‘Fuji’ apple trees. The experiment was conducted in São Joaquim, state of Santa Catarina, southern Brazil, from 2010 to 2015, in an Inceptisol soil. The three apple orchards were planted in 2004 with the Fuji cultivar grafted on Marubakaido/ M9 rootstock and grown under a high-plant-density system (1984 trees ha-1). Annual fertilizer levels of 0, 40, 80, 120 and 160 kg ha-1 of P2O5 (as triple superphosphate) were applied to the soil surface, without incorporation, in a randomized block design with five replicates. In the first and last years (2011 and 2015), soil samples were collected from 0-10, 10-20 and 0-20 cm layers and the available P content was analyzed. Annually, whole leaves were collected and analyzed for P content. The number and weight of fruits per tree and hfruit yield were also evaluated. Application of P increased the content of available P in the soil, but this increase was not accompanied by increased leaf P content nor by increased fruit yield. This suggests that, in soils with medium to high content of organic matter and well fertilized with P before planting, there is no response of apple trees for P reapplication in the 10 subsequent years.
DOI: 10.6084/m9.figshare.19944767
title: EFFECTS OF POTASSIUM SILICATE APPLICATION ON PAPAYA PLANTS IN THE TWOSPOTTED SPIDER MITE POPULATION

description: ABSTRACT The study objective was to determine whether potassium silicate application on papaya plants induces resistance to twospotted spider mite, Tetranychus urticae, and decreases its infestation. In greenhouse, the effects of potassium silicate in foliar and soil application on seedlings artificially infested, were evaluated. Two doses of potassium silicate 2 and 4L.ha-1 applied at different times were tested, resulting in treatments with different spray frequencies. Mites were submitted to three assessments, 20, 30 and 40 days after infestation, counting the number of eggs, mites and exuviae of T. urticae present along the main vein. In laboratory, fertility life tables of T. urticae fed with papaya leaves taken from plants of treatments receiving foliar application of potassium silicate were built. The residual effect was evaluated by spraying leaf discs in a Potter tower and transfer of females after 24 hours. Physiological analyses of leaves were performed to assess the physiological components indicative of activation of the defense mechanism of plants. The application of potassium silicate induced the production of plant defense substances reducing the net reproduction rate values (Ro). On the other hand, in the experiment about residual effect, it was observed hormoligosis induction with increased number of eggs per female. In the greenhouse, potassium silicate application did not result in reduced T. urticae infestation.
DOI: 10.6084/m9.figshare.19944767.v1
title: EFFECTS OF POTASSIUM SILICATE APPLICATION ON PAPAYA PLANTS IN THE TWOSPOTTED SPIDER MITE POPULATION

description: ABSTRACT The study objective was to determine whether potassium silicate application on papaya plants induces resistance to twospotted spider mite, Tetranychus urticae, and decreases its infestation. In greenhouse, the effects of potassium silicate in foliar and soil application on seedlings artificially infested, were evaluated. Two doses of potassium silicate 2 and 4L.ha-1 applied at different times were tested, resulting in treatments with different spray frequencies. Mites were submitted to three assessments, 20, 30 and 40 days after infestation, counting the number of eggs, mites and exuviae of T. urticae present along the main vein. In laboratory, fertility life tables of T. urticae fed with papaya leaves taken from plants of treatments receiving foliar application of potassium silicate were built. The residual effect was evaluated by spraying leaf discs in a Potter tower and transfer of females after 24 hours. Physiological analyses of leaves were performed to assess the physiological components indicative of activation of the defense mechanism of plants. The application of potassium silicate induced the production of plant defense substances reducing the net reproduction rate values (Ro). On the other hand, in the experiment about residual effect, it was observed hormoligosis induction with increased number of eggs per female. In the greenhouse, potassium silicate application did not result in reduced T. urticae infestation.
DOI: 10.6084/m9.figshare.19944769.v1
title: PENETRATION, DEVELOPMENT, AND REPRODUCTION OF Meloidogyne enterolobii ON Psidium SPECIES AND INDUCED CELLULAR RESPONSES IN THE ROOTS

description: ABSTRACT Meloidogyne enterolobii has severely compromised the main guava producing regions in Brazil, thereby stimulating the development of resistant varieties for efficient management of this nematode. This study aimed to assess the penetration, development, reproduction, and induced cellular responses of M. enterolobii in roots of four species of the genus Psidium (P. guajava‘Paluma’, P. guineense, P. cattleyanum, and P. friedrichstalianum) from the Active Germplasm Bank (BAG) of the Center for Agricultural Research of the Semi-arid Tropics (Embrapa Semi-arid) located in Petrolina, PE, Brazil. Three experiments were carried out to assess the penetration, development, and reproduction of the nematode and, subsequently, histological analyses were performed. Nematode penetrated indistinctly in both resistant and susceptible species, but only in ‘Paluma’ an evolution was observed from vermiform juveniles to “sausage” and globose forms in experiment 1. In experiment 2, all species presented “sausage” forms of nematode, but only in ‘Paluma’, these forms reached adult female stages. In experiment 3, only ‘Paluma’ was susceptible to M. enterolobii, with reproduction factor above unity. Histological analyses showed that, except for P. guajava, the other Psidium species presented poorly developed feeding sites at 20 days after inoculation.
DOI: 10.6084/m9.figshare.19944769
title: PENETRATION, DEVELOPMENT, AND REPRODUCTION OF Meloidogyne enterolobii ON Psidium SPECIES AND INDUCED CELLULAR RESPONSES IN THE ROOTS

description: ABSTRACT Meloidogyne enterolobii has severely compromised the main guava producing regions in Brazil, thereby stimulating the development of resistant varieties for efficient management of this nematode. This study aimed to assess the penetration, development, reproduction, and induced cellular responses of M. enterolobii in roots of four species of the genus Psidium (P. guajava‘Paluma’, P. guineense, P. cattleyanum, and P. friedrichstalianum) from the Active Germplasm Bank (BAG) of the Center for Agricultural Research of the Semi-arid Tropics (Embrapa Semi-arid) located in Petrolina, PE, Brazil. Three experiments were carried out to assess the penetration, development, and reproduction of the nematode and, subsequently, histological analyses were performed. Nematode penetrated indistinctly in both resistant and susceptible species, but only in ‘Paluma’ an evolution was observed from vermiform juveniles to “sausage” and globose forms in experiment 1. In experiment 2, all species presented “sausage” forms of nematode, but only in ‘Paluma’, these forms reached adult female stages. In experiment 3, only ‘Paluma’ was susceptible to M. enterolobii, with reproduction factor above unity. Histological analyses showed that, except for P. guajava, the other Psidium species presented poorly developed feeding sites at 20 days after inoculation.
DOI: 10.6084/m9.figshare.5666935
title: PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

description: ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las.
DOI: 10.6084/m9.figshare.5666935.v1
title: PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

description: ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las.
DOI: 10.6084/m9.figshare.5667394
title: IS Annona emarginata CAPABLE OF ACCUMULATE ESSENTIAL HEAVY METALS WITHOUT DAMAGES IN THE METABOLISM?

description: ABSTRACT This study aimed to evaluate if ionic strength variation causes differential accumulation of heavy metals in plants of Annona emarginata (Schltdl.) H. Rainer var. ‘terra-fria’, modifying gas exchange and dry matter production. The seedlings were cultivated under different ionic strengths of the ‘Hoagland and Arnon’s’ nutrient solution (75% I, 50% I and 25% I). At 180 days after the application of the treatments, the effects of the essential heavy metals on the physiological parameters and foliar ionic concentration were assessed. The leaf gas exchanges and vegetative growth were affected by the variation in the essential heavy metals, with positive effects on the plants that were treated with 75%I. The seedlings that were grown under 75% I presented higher concentrations of Zn (24.2mg kg-1). On the other hand Fe (453mg kg-1) e Mn (803.5mg kg-1) accumulated more under 25% I, but gas exchanges and vegetative growth were reduced, compared to 50%I and 75%I. We can conclude that the ionic strength variation alters gas exchange and the dry matter accumulation, causing differential accumulation of Fe, Mn and Zn in A. emarginata, occurring inverse relationship between increased ionic strength and Fe and Mn content, besides directly relationship to the Zn content.
DOI: 10.6084/m9.figshare.5667394.v1
title: IS Annona emarginata CAPABLE OF ACCUMULATE ESSENTIAL HEAVY METALS WITHOUT DAMAGES IN THE METABOLISM?

description: ABSTRACT This study aimed to evaluate if ionic strength variation causes differential accumulation of heavy metals in plants of Annona emarginata (Schltdl.) H. Rainer var. ‘terra-fria’, modifying gas exchange and dry matter production. The seedlings were cultivated under different ionic strengths of the ‘Hoagland and Arnon’s’ nutrient solution (75% I, 50% I and 25% I). At 180 days after the application of the treatments, the effects of the essential heavy metals on the physiological parameters and foliar ionic concentration were assessed. The leaf gas exchanges and vegetative growth were affected by the variation in the essential heavy metals, with positive effects on the plants that were treated with 75%I. The seedlings that were grown under 75% I presented higher concentrations of Zn (24.2mg kg-1). On the other hand Fe (453mg kg-1) e Mn (803.5mg kg-1) accumulated more under 25% I, but gas exchanges and vegetative growth were reduced, compared to 50%I and 75%I. We can conclude that the ionic strength variation alters gas exchange and the dry matter accumulation, causing differential accumulation of Fe, Mn and Zn in A. emarginata, occurring inverse relationship between increased ionic strength and Fe and Mn content, besides directly relationship to the Zn content.
DOI: 10.6084/m9.figshare.19944770
title: BOTÂNICA E FISIOLOGIA/ BOTANY AND PHYSIOLOGY ANTIOXIDANT ACTIVITY AND PHYSICOCHEMICAL PARAMETERS IN ‘CUERNAVAQUEÑA’ MEXICAN PLUM (Spondias purpurea L.) AT DIFFERENT RIPENING STAGES

description: ABSTRACT Mexican plum (Spondias purpurea L.) ‘Cuernavaqueña’ was harvested at four ripening stages, with the aim of evaluating the concentration of bioactive compounds and antioxidant capacity in the pulp and the epicarp. The highest ethylene production (9.43 mL kg h-1) and total soluble solids concentration (23.9 °Brix) was observed in the fully ripe stage. Titratable acidity was higher in green stage compared to other analyzed ripening stages in both pulp (0.48 %) and epicarp (0.32 %). Fully ripe plum epicarp presented the highest content of total phenols (GAE 190 mg g-1), flavonoids (QE 214 mg g-1), and carotenoids (853 mg g-1) compared to other ripening stages. The antioxidant capacity was higher in the epicarp of the fully ripe fruit compared to the other ripening stages: DPPH (1087 µM TE /100 g), ABTS (1534 µM TE/100 g), and FRAP (1764 µM TE/100 g). Significant correlations (r = 0.60 *** to 0.95 **) between bioactive compounds concentrations and antioxidant activity were obtained.
DOI: 10.6084/m9.figshare.19944770.v1
title: BOTÂNICA E FISIOLOGIA/ BOTANY AND PHYSIOLOGY ANTIOXIDANT ACTIVITY AND PHYSICOCHEMICAL PARAMETERS IN ‘CUERNAVAQUEÑA’ MEXICAN PLUM (Spondias purpurea L.) AT DIFFERENT RIPENING STAGES

description: ABSTRACT Mexican plum (Spondias purpurea L.) ‘Cuernavaqueña’ was harvested at four ripening stages, with the aim of evaluating the concentration of bioactive compounds and antioxidant capacity in the pulp and the epicarp. The highest ethylene production (9.43 mL kg h-1) and total soluble solids concentration (23.9 °Brix) was observed in the fully ripe stage. Titratable acidity was higher in green stage compared to other analyzed ripening stages in both pulp (0.48 %) and epicarp (0.32 %). Fully ripe plum epicarp presented the highest content of total phenols (GAE 190 mg g-1), flavonoids (QE 214 mg g-1), and carotenoids (853 mg g-1) compared to other ripening stages. The antioxidant capacity was higher in the epicarp of the fully ripe fruit compared to the other ripening stages: DPPH (1087 µM TE /100 g), ABTS (1534 µM TE/100 g), and FRAP (1764 µM TE/100 g). Significant correlations (r = 0.60 *** to 0.95 **) between bioactive compounds concentrations and antioxidant activity were obtained.
DOI: 10.6084/m9.figshare.5719156.v1
title: FREMONT - IAC 543: TANGERINE WITH POTENTIAL FOR THE BRAZILIAN MARKET

description: ABSTRACT Planting new types of tangerines is an interesting alternative for Brazilian citrus growers due to the increasing domestic market. Thus, the aim of this study was to evaluate a less exploited variety of tangerine, the Fremont. The physics and chemical quality of the fruit and juice (mass, peel color, juice yield, acidity, soluble solids and maturation index) was evaluated, followed by sensory testing for ranking of difference and preference to Nules clementine and Ponkan tangerine varieties. The Fremont tangerine produces fruits that meet the standards required by the Brazilian consumer market, with an appropriate mass for sale of the fresh fruit, a suitable juice yield, and early to mid-season maturation. Furthermore, et the preference, together with the Nules clementine, when compared to the Ponkan tangerine, wich is the main commercial variety in Brazil.
DOI: 10.6084/m9.figshare.5719156
title: FREMONT - IAC 543: TANGERINE WITH POTENTIAL FOR THE BRAZILIAN MARKET

description: ABSTRACT Planting new types of tangerines is an interesting alternative for Brazilian citrus growers due to the increasing domestic market. Thus, the aim of this study was to evaluate a less exploited variety of tangerine, the Fremont. The physics and chemical quality of the fruit and juice (mass, peel color, juice yield, acidity, soluble solids and maturation index) was evaluated, followed by sensory testing for ranking of difference and preference to Nules clementine and Ponkan tangerine varieties. The Fremont tangerine produces fruits that meet the standards required by the Brazilian consumer market, with an appropriate mass for sale of the fresh fruit, a suitable juice yield, and early to mid-season maturation. Furthermore, et the preference, together with the Nules clementine, when compared to the Ponkan tangerine, wich is the main commercial variety in Brazil.
DOI: 10.6084/m9.figshare.5718628
title: POST-ACIDIFICATION AND EVALUATION OF ANTHOCYANINS STABILITY AND ANTIOXIDAN ACTIVITY IN AÇAI FERMENTED MILK AND YOGURTS (Euterpe oleracea Mart.)

description: ABSTRACT This study evaluated the post-acidification, stability of anthocyanins and antioxidant activity in açai yogurts and fermented milks for 28 days of cold storage. For the determination of post-acidification and stability of the functional properties of açai yogurt (IA) and fermented milks (LFA), products stored at 4°C were evaluated on day 0 and every 7 days for pH, titratable acidity in lactic acid, instrumental color, anthocyanins and antioxidant activity by the DPPH free radical method. Acidification of both food matrixes was more evident between days 0 and 7 of evaluation. IA presented reduction in parameter L *, while chromaticity a * and b * of IA and LFA increased as the Açai pulp content increased; however, considering the evaluation period, it was found increase in L * and b * relating to anthocyanin degradation. Anthocyanins, as well as the antioxidant activity of IA and LFA, showed an increase in their values as the pulp content increased, but their concentrations reduced at the end of the storage period. IA and LFA can be considered excellent sources of antioxidants, being alternative to individuals not used to the consumption of fruits and vegetables.