cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8259731
title: Functional and technological attributes of probiotic yoghurt prepared with Dahi micro-flora during refrigerated storage

description: Abstract In the present investigation functional, technological, and rheological attributes of probiotic yoghurt were studied during refrigerated storage (4 °C) for 28 days. The different preparations of probiotic yoghurts were made from dahi microflora which was documented as probiotics. Three strains of lactic acid bacteria (LAB) i.e. S. thermophilus (S02FT), L. bulgaricus (TLB06FT) and L. acidophilus (LA06FT) were selected for formulations of probiotic yoghurt. Three sets of probiotic yoghurt including Y1: S02FT and TLB06FT (1:1ratio), Y2: S02FT and TLB06FT (1:2ratio) and Y3: S02FT, TLB06FT and LA06FT (1:1:1 ratio) along with Y0 as a control (non-probiotic strains) were prepared and stored at 4 °C for 28 days. The results showed that technological attributes of Y3 preparation had better lactic acid production (75% at 0 day and 93% at 28 days) as compared to control. Moreover, Y3 preparation had lesser synersis (20% at 0 day and 35% at 28days) as compared to other treatments i.e. Y1, Y2, Y0 that had 24, 70 and 18% synersis at 0 day of storage respectively). However, viscosity of Y3 probiotic yoghurt indicated higher values (3001 cP at 0 days and 1310 cP at 28 days) among the rest of preparations. Functional attributes of Y3 showed the better results such as viability of probiotics (40×10-6 CFU/ml at 0 day and 1.16×10-6 CFU/ml at 28 days) and antibacterial activity (7.5 mm at 0 days 7 mm at 28 days) compared to other preparations. Conclusively, Y3 formulation attained the higher sensory attributes due to its better lactic acid production, viscosity and lesser synersis values during entire study duration in formulated yoghurt.
DOI: 10.6084/m9.figshare.8259734
title: Kidney Disease in Diabetes Mellitus: Cross-Linking between Hyperglycemia, Redox Imbalance and Inflammation

description: Abstract Chronic hyperglycemia is the key point of macro- and microvascular complications associated with diabetes mellitus. Excess glucose is responsible for inducing redox imbalance and both systemic and intrarenal inflammation, playing a critical role in the pathogenesis of diabetic kidney disease, which is currently the leading cause of dialysis in the world. The pathogenesis of the disease is complex, multifactorial and not fully elucidated; many factors and mechanisms are involved in the development, progression and clinical outcomes of the disease. Despite the disparate mechanisms involved in renal damage related to diabetes mellitus, the metabolic mechanisms involving oxidative/inflammatory pathways are widely accepted. The is clear evidence that a chronic hyperglycemic state triggers oxidative stress and inflammation mediated by altered metabolic pathways in a self-perpetuating cycle, promoting progression of cell injury and of end-stage renal disease. The present study presents an update on metabolic pathways that involve redox imbalance and inflammation induced by chronic exposure to hyperglycemia in the pathogenesis of diabetic kidney disease.
DOI: 10.6084/m9.figshare.8259731.v1
title: Functional and technological attributes of probiotic yoghurt prepared with Dahi micro-flora during refrigerated storage

description: Abstract In the present investigation functional, technological, and rheological attributes of probiotic yoghurt were studied during refrigerated storage (4 °C) for 28 days. The different preparations of probiotic yoghurts were made from dahi microflora which was documented as probiotics. Three strains of lactic acid bacteria (LAB) i.e. S. thermophilus (S02FT), L. bulgaricus (TLB06FT) and L. acidophilus (LA06FT) were selected for formulations of probiotic yoghurt. Three sets of probiotic yoghurt including Y1: S02FT and TLB06FT (1:1ratio), Y2: S02FT and TLB06FT (1:2ratio) and Y3: S02FT, TLB06FT and LA06FT (1:1:1 ratio) along with Y0 as a control (non-probiotic strains) were prepared and stored at 4 °C for 28 days. The results showed that technological attributes of Y3 preparation had better lactic acid production (75% at 0 day and 93% at 28 days) as compared to control. Moreover, Y3 preparation had lesser synersis (20% at 0 day and 35% at 28days) as compared to other treatments i.e. Y1, Y2, Y0 that had 24, 70 and 18% synersis at 0 day of storage respectively). However, viscosity of Y3 probiotic yoghurt indicated higher values (3001 cP at 0 days and 1310 cP at 28 days) among the rest of preparations. Functional attributes of Y3 showed the better results such as viability of probiotics (40×10-6 CFU/ml at 0 day and 1.16×10-6 CFU/ml at 28 days) and antibacterial activity (7.5 mm at 0 days 7 mm at 28 days) compared to other preparations. Conclusively, Y3 formulation attained the higher sensory attributes due to its better lactic acid production, viscosity and lesser synersis values during entire study duration in formulated yoghurt.
DOI: 10.6084/m9.figshare.8259734.v1
title: Kidney Disease in Diabetes Mellitus: Cross-Linking between Hyperglycemia, Redox Imbalance and Inflammation

description: Abstract Chronic hyperglycemia is the key point of macro- and microvascular complications associated with diabetes mellitus. Excess glucose is responsible for inducing redox imbalance and both systemic and intrarenal inflammation, playing a critical role in the pathogenesis of diabetic kidney disease, which is currently the leading cause of dialysis in the world. The pathogenesis of the disease is complex, multifactorial and not fully elucidated; many factors and mechanisms are involved in the development, progression and clinical outcomes of the disease. Despite the disparate mechanisms involved in renal damage related to diabetes mellitus, the metabolic mechanisms involving oxidative/inflammatory pathways are widely accepted. The is clear evidence that a chronic hyperglycemic state triggers oxidative stress and inflammation mediated by altered metabolic pathways in a self-perpetuating cycle, promoting progression of cell injury and of end-stage renal disease. The present study presents an update on metabolic pathways that involve redox imbalance and inflammation induced by chronic exposure to hyperglycemia in the pathogenesis of diabetic kidney disease.
DOI: 10.6084/m9.figshare.8259722
title: Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

description: Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN, and good specificity, with less than 0.5% of cross-reactivity towards DAN structurally related compounds. The developed immunoaffinity column had an approximate binding capacity of 2300 ng/mL gel and a relative standard deviation (RSD) of 3.7% among columns. The immunoaffinity columns were then challenged with DAN-fortified milk samples, recoveries of DAN were found to be in the range of 83.8% to 98.8%, indicating the prepared immunoaffinity column is suitable for use as an efficient extraction method to detect DAN residues in food products.
DOI: 10.6084/m9.figshare.8259728
title: Effect of the essential oil of Croton nepetifolius Baill on cervical penetration in crossbred Dorper and Santa Inês ewes

description: ABSTRACT In order to evaluate the use of the essential oil of Croton nepetifolius Baill (EOCn) on cervical penetration in crossbred ewes, 40 ewes were randomly allocated into four groups (n= 10): CONTROL, MISOPROSTOL, EOCn50 and EOCn100 (50 e 100µg of the EOCn, respectively). After estrus synchronization, using CIDR and eCG (200IU), depth of cervical penetration was measured using artificial insemination gun for bovine species which was graduated and used from 0 to 72h after CIDR removal. Results were expressed as mean ± standard error mean, submitted to ANOVA and Tukey test while data in percentage were submitted to Fisher or Chi-Square test. No significant difference (P&gt; 0.05) was observed at grade of cervical penetration. Concerning trespassing time, MISOPROTOL and EOCn100 groups presented a lower trespassing time at 60h (1.7±0.6 and 1.5±0.6min, respectively) than CONTROL group (4.1±0.6min), which did not differ significantly from EOCn50 (2.3±0.6min) group. Therefore, the essential oil of Croton nepetifolius Baill can be used to shorten the cervical penetration time in estrus synchronized ewes.
DOI: 10.6084/m9.figshare.8259716
title: Heritability estimation and genetic correlations for mature weight, visual scores, and growth traits in Nellore cattle

description: ABSTRACT This study was intended to estimate the genetic associations between growth traits and visual scores with possible changes in mature weight (MW) in 397,900 Nellore animals. A bi-character analysis was performed to estimate the (co)variances and genetic parameters for MW, which comprises the following traits: conformation, finishing precocity, musculature at weaning (WC, WP, and WM) and yearling (YC, YP, and YM), birth weight (BW), weight from birth to weaning (WG), weight from weaning to yearling (YG), and final index (FIND). The observed mean MW was 417.6±56.2 kg, and the direct genetic effect mean estimated heritability ( h 2 a 1) of MW was 0.45. Overall, the BW was 31.0±3.7 kg, and the estimated h² was 0.34. The heritability estimate of the maternal additive genetic component ( h 2 m 2) of BW was 0.07. We calculated the mean WG to be 144.1±26.3 and estimated the h 2 a 2 as 0.18 and h 2 m 2 as 0.07. The value for h 2 a YG (0.17) and YW (0.26) were also estimated. The heritability of the weaning WC (0.17), WP (0.19), and WM (0.17) and yearling YC (0.25), YP (0.27), and YM (0.25) were estimated using visual scores. The h 2 m values for weaning WC, WP, and WM (0.06) with visual scores were estimated. The genetic correlations between body weight (BoW) at YC and WC (0.62) were considered moderately high and positive. In addition, YP (0.18), YM (0.15), WP (0.13), and WM (0.14) were considered moderately low compared with MW. The genetic correlation between BW and FIND (0.38) was considered positive and moderate. The heritability estimation indicates that growth traits, visual scores, and weight of adult cows could be changed by selection. Cows that presented the highest h 2 a values for live weight responded rapidly to selection based on growth characteristics, visual scores, and FIND and might result in increased final MW.
DOI: 10.6084/m9.figshare.8259728.v1
title: Effect of the essential oil of Croton nepetifolius Baill on cervical penetration in crossbred Dorper and Santa Inês ewes

description: ABSTRACT In order to evaluate the use of the essential oil of Croton nepetifolius Baill (EOCn) on cervical penetration in crossbred ewes, 40 ewes were randomly allocated into four groups (n= 10): CONTROL, MISOPROSTOL, EOCn50 and EOCn100 (50 e 100µg of the EOCn, respectively). After estrus synchronization, using CIDR and eCG (200IU), depth of cervical penetration was measured using artificial insemination gun for bovine species which was graduated and used from 0 to 72h after CIDR removal. Results were expressed as mean ± standard error mean, submitted to ANOVA and Tukey test while data in percentage were submitted to Fisher or Chi-Square test. No significant difference (P&gt; 0.05) was observed at grade of cervical penetration. Concerning trespassing time, MISOPROTOL and EOCn100 groups presented a lower trespassing time at 60h (1.7±0.6 and 1.5±0.6min, respectively) than CONTROL group (4.1±0.6min), which did not differ significantly from EOCn50 (2.3±0.6min) group. Therefore, the essential oil of Croton nepetifolius Baill can be used to shorten the cervical penetration time in estrus synchronized ewes.
DOI: 10.6084/m9.figshare.8259713
title: Quality changes in reared, hot-smoked meagre (Argyrosomus regius Asso, 1801) during chill storage at 4 ± 1 °C

description: Abstract The aim of this study was to investigate quality and nutritional changes in reared meagre after the use of hot smoking technology. Hot smoking method was applied to the meagre and the resulting smoked fish were used to produce fillets, which were stored in refrigerator after being vacuum packed. As a result of chemical component analysis, it was seen that the fat content was low. In particular, the PUFA contents of meagre were found to be very high (35.53% Control). During storage, changes in fatty acid composition and the chemical, sensory and microbiological changes were analyzed. In terms of the chemical quality indicated, pH, TBA and TMA values didn’t exceed limit values during the storage of both fresh (6.25, 0.44 mgMDA/kg and 4.50 mg/100g) and hot smoked fish (6.23, 1.12 mgMDA/kg,5.57mg/100g respectively), but the TVB-N value exceeded limit values at day 56 of storage for hot smoked samples (39.50mg/100g). According to microbiological limit values accepted for seafood fresh and hot smoked meagre lost consumption characteristics after day 7 and day 35 of storage, respectively. Meagre was determined to be appropriate for the hot smoking process, based on the quality analyses, especially sensory analyses.
DOI: 10.6084/m9.figshare.8259722.v1
title: Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

description: Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN, and good specificity, with less than 0.5% of cross-reactivity towards DAN structurally related compounds. The developed immunoaffinity column had an approximate binding capacity of 2300 ng/mL gel and a relative standard deviation (RSD) of 3.7% among columns. The immunoaffinity columns were then challenged with DAN-fortified milk samples, recoveries of DAN were found to be in the range of 83.8% to 98.8%, indicating the prepared immunoaffinity column is suitable for use as an efficient extraction method to detect DAN residues in food products.
DOI: 10.6084/m9.figshare.8259710
title: Antioxidant activity and lipid oxidation in milk from cows with soybean oil and propolis extract added to their feed

description: Abstract The presence of PUFA (polyunsaturated fatty acids) in cow milk, including conjugated linoleic acids (CLA) isomers, makes milk susceptible to lipid oxidation, and may lead to the formation of volatile products responsible for a rancid smell. Consequently, including natural antioxidants in the diet of dairy cattle can assist in preventing lipid oxidation. In this study, soybean oil (a source of PUFA) and different ethanolic extracts of Brazilian propolis were included in the cows' feed, and the antioxidant activity and lipid oxidation of the animals' milk was assessed. Antioxidant activity was analyzed using the DPPH method, potassium ferricyanide reduction and chelating ability. Total phenolic compounds and flavonoids were also determined. Lipid oxidation was assessed by determining conjugated dienes and the production of aldehydes (propanal, pentanal, hexanal and octanal). Increased antioxidant activity was seen in the samples of milk from cows receiving propolis. Similarly, the addition of ethanolic extract of propolis to the diet of dairy cattle increased milk protection for two treatments with propolis against lipid oxidation in terms of production of propanal and hexanal. These results confirm the positive effect of applying this natural additive to the diet of lactating cows, improving the nutritional quality of the milk produced.
DOI: 10.6084/m9.figshare.8259716.v1
title: Heritability estimation and genetic correlations for mature weight, visual scores, and growth traits in Nellore cattle

description: ABSTRACT This study was intended to estimate the genetic associations between growth traits and visual scores with possible changes in mature weight (MW) in 397,900 Nellore animals. A bi-character analysis was performed to estimate the (co)variances and genetic parameters for MW, which comprises the following traits: conformation, finishing precocity, musculature at weaning (WC, WP, and WM) and yearling (YC, YP, and YM), birth weight (BW), weight from birth to weaning (WG), weight from weaning to yearling (YG), and final index (FIND). The observed mean MW was 417.6±56.2 kg, and the direct genetic effect mean estimated heritability ( h 2 a 1) of MW was 0.45. Overall, the BW was 31.0±3.7 kg, and the estimated h² was 0.34. The heritability estimate of the maternal additive genetic component ( h 2 m 2) of BW was 0.07. We calculated the mean WG to be 144.1±26.3 and estimated the h 2 a 2 as 0.18 and h 2 m 2 as 0.07. The value for h 2 a YG (0.17) and YW (0.26) were also estimated. The heritability of the weaning WC (0.17), WP (0.19), and WM (0.17) and yearling YC (0.25), YP (0.27), and YM (0.25) were estimated using visual scores. The h 2 m values for weaning WC, WP, and WM (0.06) with visual scores were estimated. The genetic correlations between body weight (BoW) at YC and WC (0.62) were considered moderately high and positive. In addition, YP (0.18), YM (0.15), WP (0.13), and WM (0.14) were considered moderately low compared with MW. The genetic correlation between BW and FIND (0.38) was considered positive and moderate. The heritability estimation indicates that growth traits, visual scores, and weight of adult cows could be changed by selection. Cows that presented the highest h 2 a values for live weight responded rapidly to selection based on growth characteristics, visual scores, and FIND and might result in increased final MW.
DOI: 10.6084/m9.figshare.8259701
title: Protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury in rats

description: Abstract This study investigated the protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury (MIRI) in rats. Sixty SD rats were randomly divided into sham-operated, model, and low-, medium- and high-dose crocetin groups. Later 3 groups were intragastrically administrated with 10, 20 and 40 mg/kg crocetin from Crocus sativus L., respectively, for 1 week. On the 8th day, the MIRI model was established in the later 4 groups. The blood biochemical indexes, hemodynamic indexes, myocardial infarct size, myocardial antioxidant indexes and myocardial expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), serine/threonine kinase (Akt) and phosphorylated Akt (p-Akt) protein were determined. Results showed that, compared with model group, in high-dose crocetin group the left ventricular systolic pressure, +dp/dtmax and -dp/dtmax, myocardial superoxide dismutase and glutathione peroxidase levels, and myocardial Bcl-2/Bax ratio and p-Akt/Akt ratio were significantly increased, and the left ventricular end diastolic pressure, myocardial infarct size, serum lactate dehydrogenase, creatine kinase-MB, cardiac troponin I, tumor necrosis factor-α, interleukin-1β, interleukin-6 and malondialdehyde levels were significantly decreased (all P &lt; 0.05). In conclusion, crocetin from Crocus sativus L. has protective effects on MIRI in rats.
DOI: 10.6084/m9.figshare.8259713.v1
title: Quality changes in reared, hot-smoked meagre (Argyrosomus regius Asso, 1801) during chill storage at 4 ± 1 °C

description: Abstract The aim of this study was to investigate quality and nutritional changes in reared meagre after the use of hot smoking technology. Hot smoking method was applied to the meagre and the resulting smoked fish were used to produce fillets, which were stored in refrigerator after being vacuum packed. As a result of chemical component analysis, it was seen that the fat content was low. In particular, the PUFA contents of meagre were found to be very high (35.53% Control). During storage, changes in fatty acid composition and the chemical, sensory and microbiological changes were analyzed. In terms of the chemical quality indicated, pH, TBA and TMA values didn’t exceed limit values during the storage of both fresh (6.25, 0.44 mgMDA/kg and 4.50 mg/100g) and hot smoked fish (6.23, 1.12 mgMDA/kg,5.57mg/100g respectively), but the TVB-N value exceeded limit values at day 56 of storage for hot smoked samples (39.50mg/100g). According to microbiological limit values accepted for seafood fresh and hot smoked meagre lost consumption characteristics after day 7 and day 35 of storage, respectively. Meagre was determined to be appropriate for the hot smoking process, based on the quality analyses, especially sensory analyses.
DOI: 10.6084/m9.figshare.8259704
title: Optimization of extraction process for efficient imino acids recovery and purification from low-value sea cucumber

description: Abstract For high recoveries and purities of L-proline (L-Pro) and L-hydroxyproline (L-Hyp) from the low-value sea cucumber Acaudina leucoprocta, the extraction process was systematically investigated and optimized. The results indicated that, in decoloration of A. leucoprocta hydrolysate, activated carbon powdered W660 was more suitable due to the low addition amount and short treatment time compared with macroporous resin HZ816 offering comparable decoloration ratio and imino acids recovery yields. For oxidative deamination, HCl-HNO3 exhibited better performance than NaNO2 with complete primary amino acids broken-down and fewer imino acids loss. After decoloration and oxidative deamination under their optimized conditions, an extraction solution was obtained with L-Pro and L-Hyp recovery yields of 76.3% and 78.8% from A. leucoprocta hydrolysate, which was directly used for separation of L-Pro and L-Hyp with ion-exchange chromatograph. This work may be referenced for the large-scale L-Pro and L-Hyp production from other low-value materials.
DOI: 10.6084/m9.figshare.8259707
title: Ammonium chloride as an alternative source of nitrogen for sugarcane during two consecutive cycles

description: Abstract: The objective of this work was to evaluate the effect of the application of ammonium chloride as a nitrogen source for sugarcane (Saccharum sp.) on the development of the crop’s shoot and roots during two consecutive cycles. The experiment was conducted on a Typic Hapludox in the first and second sugarcane ratoons. The treatments consisted of four N doses (50, 100, 150, and 200 kg ha-1) applied as ammonium chloride (NH4Cl), besides a control, without the application of N. The increase in the NH4Cl dose increased in up to three times soil electrical conductivity in the layer of 0.0-0.2 m, with a consequent increase in the concentration of chlorine in leaves. In the second cycle, the contents of chlorine increased in 1,900 mg kg-1 in the leaf with the application of 200 mg kg-1 NH4Cl, when compared with the control. In the first cycle, the application of NH4Cl doses above 200 kg ha-1 N promoted a decrease in shoot productivity. In both cycles, there was no effect of the NH4Cl doses on root dry matter. High doses of NH4Cl, in consecutive sugarcane cycles, result in a decrease in the productivity of stalks for processing.