DOI: 10.20350/digitalcsic/14780
title: Raw data of manuscript ???Nitro-oleic acid regulates T cell activation through post-translational modification of calcineurin???

description: Methods used for generation of data are described under the Material and Methods section of the manuscript and the Supplementary Methods of the Supplementary Information Text.
description: MAIN FIGURES Figure 1: Figure 1A: Exp1: ???Histogram_Exp1??? and corresponding FACSfiles. Exp2: ???Histogram_Exp2???, ???Hig resolution???, ???LowResolution_Exp2??? and corresponding FACSfiles. Exp3: ???Histogram Exp3??? and corresponding FACSfiles. Exp4: ???Histogram Exp4??? and corresponding FACSfiles. ???Fig1A_Graph???. ???Fig1A_Histogram???. ???Graph_Fig1A???. Figure 1B: Exp1: FACS files corresponding to CD69 and CD71. Exp2: FACS files corresponding to CD69 and CD71. Exp3: FACS files corresponding to CD69 and CD71. Fig1B_HistogramsCD71 and CD69: FACS files corresponding to Figure1B. ???Fig1B_Graph???. ???Graph_Fig1B???. Figure 1C: CTV CD4 CD8: FACS files corresponding to cell proliferation. CTV CD4 CD8II: FACS files corresponding to cell proliferation. Histogram_Fig 1C: FACS files and Histogram Figure 1C. Figure 1D: ???Graph_Fig1D???. ???Fig1D_Graph???. Figure 1E_F: Exp1: FACS files corresponding to Figure 1E and F. Exp2: FACS files corresponding to Figure 1E and F. Exp3: FACS files corresponding to Figure 1E and F. ???Fig1E_Graph??? ???Fig1F_Graph??? ???Graph_Fig1E_F??? ???Layout Th2??? ???Layout Th1??? Figure 2 Figure 2A: ???Data RT-PCR IL2IFNGIL4vsGAPDH Fig 2A??? ???RT-PCR IFNg vs GAPDH 100%??? ???RT-PCR IL2 vs GAPDH 100%??? ???RT-PCR IL4 vs GAPDH 100%??? Figure 2B: IFNgData: ???Data Expts IFNgLUC??? and ???Raw Data Fig 2B IFNgLuc??? IL2Data: ???Data Expts IL2LUC??? and ???Raw Data Fig 2B IL2Luc??? IL4Data: ???Data Expts IL4LUC??? and ???Raw Data Fig 2B IL4Luc??? Figure 3 Figure 3A: NFATLucPMA+Ion: ???Graph_Fig3A_PMAI??? and ???GraphFig3A_PMAI???. NFATLucRaji+SEB: ???Graph_Fig3A_SEB??? and ???GraphFig3A_SEB???. Figure 3B: ???Fig3B_Graph_white??? and ???Graph_Fig3B??? Figure 3C: ???Fig3C_Graph??? and ???Graph_Fig3C??? Figure 3D: Exp: b-actin: 2 files WB b-actin. RCAN: 4 files WB inducible and constitutive RCAN. Quantification: 3 quantifications (3 image files, 3 graphs files and 3 excel files). Excel ???Densitometric analysis_bActin???. Fig 3D. Figure 4 Figure 4A: Photomicrographs_Fig4A: imaging files. Quantitative imaging: imaging files analysed. ???Nuclear_NFAT percentage???. ???Quantification??? Excel file. Figure 4B: Exp: Cytosol: 2 files WB dynamin II and 2 files WB NFATC2. Nucleus: 2 files WB LaminB1 and 2 files WB NFATC2. ???Figure 4B???. Figure 4C: Exp: DynaminII: 3 files WB Dynamin II. NFAT: 4 files WB NFATC2. ???Fig4C??? Figure 4D: ???CaNA_DCAM domains???. Figure 4E: ???Graph_Fig4E??? and ???Fig4E_Graph???. Figure 4F: ???Graph_Fig4F??? and ???Fig4F_Graph???. Figure 4G: ???Graph_Fig4G??? and ???Fig4G_Graph???. Figure 4H: Exp: 5 FACSfiles and ???Fig4H???. Figure 5 Figure 5A: Exp: 2 files WB nitroalkylation and 1 file WB Coomassie CaNA. ???Fig5A???. Figure 5B: Exp: 2 files WB nitroalkylation and 1 file WB Coomassie DCAM- AI. ???Fig 5B???. Figure 5C: ???NitroalkylAA_Sequence_CaNA???. Figure 5D: ???Graph_Fig5D???. ???Dots Fig 5D???. ???Fig5D??? Figure 5E: ???Graph_Fig5E??? and ???Fig5E_Graph???. Figure 5F: Exp: 4 files WB Biotin_Nitroalkylation and 1 file Coomassie GST-DCAM. ???Fig 5F???. ???Quantification???. Figure 6 Figure 6A: Exp: CoIPCnB: 2 files WB CaNB. InputCnA: 4 files WB input CaNA. Input CaNB: 1 file WB input CaNB. IPCnA: 1 file WB IP CaNA. ???Fig 6A???. ???Quantification co_IP Fig6A???. Figure 6B: Exp: 2 files WB CaNB. 1 file WB input CaNB. 1 file Coomassie GST-DCAM-AI. ???Fig 6B???. ???quantification pull_down_Fig6B???. Figure 6C: Exp: 2 files WB CaNB. 1 file Input CaNB. 1 file Coomassie GST-DCAM-AI. ???Fig 6C???. ???Quantification Fig6C???. Figure 6D: Exp: CoIP_CaNB: 3 files WB CaNB. Input_CaNB: 1 file WB input CaNB. Input_DCAM_GFP: 2 files WB GFP-DCAM-AI. IP_DCAM_GFP: 2 files WB GFP-DCAM-AI. ???Fig6D???. ???Quantification Co_IPFig6D???. SUPORTING INFORMATION FIGURES SI_Figure 1 Exp1: ???Histogram Exp1??? and corresponding FACSfiles. Exp2: ???Histogram Exp2??? and corresponding FACSfiles. Exp3: ???Histogram Exp3??? and corresponding FACSfiles. ???Graph_SI_Fig1B???. ???Histogram_SI_Fig1A???. ???Kinetics_CD25_Donor1???. ???Kinetics_CD25_Donor2???. ???Kinetics_CD25_Donor3???. SI Figure 2 T lymphoblasts Annexin 7AAD: Dot plot analysis and corresponding FACSfiles. ???SI_Fig2???. SI Figure 3 SI_Fig 3A: NFkB PMA+Ion: ???Graph_FigS3_PMAI??? and ???GraphFigS3PMAI???. NFkBLuc Raji+SEB: ???Graph_FigS3A_SEB??? and ???FigS3A_SEB_Graph???. SI_Fig 3B: WB Figure 2 SI: 2 files WB IKB and p-IKB. ???Fig_S3B???. SI_Figure 4: ???Mass Spectra???. SI Figure 5 SI_Fig5A: ???DCAM_Mutants??? SI_Fig5B: b_actin_129_228_266_372_GFP: 4 files WB Actin. b_actin_153_178_184_256_GFP: 3 files WB Actin. DCAM_129_228_266_372_GFP: 4 files WB DCAM mutants. DCAM_153_178_184_256_GFP: 3 files WB DCAM mutants. ???Expression of mutants I_153_178_184_256_GFP???. ???Expression of mutants II_129_228_266_372_GFP???. SI Figure 6 SI_Fig6A: ???Mass Spectrometry DCAM-GFP???. SI_Fig6BanC: ???SI_Fig6BandC???. SI Figure 7 SI_Fig 7A: Exp: CaNB: 2 files WB CaNB. Input CaNB: 2 files WB input CaNB. ???Densitometric analysis???. ???Dose_binding Graph???. ???SI_Fig7A???. SI_Fig 7B: Exp: CaNA: 2 files Coomassie GST-DCAM and 3 files corresponding quantification. CaNB: 5 files WB bound CaNB and 6 files Quantification. ???Quantification_BindingCaNB_to_CaNA_kinetics???. ???SI-Fig_7B???