DOI: 10.5061/dryad.9gs8k
title: Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea

description: Whilst genotype-environment interaction is increasingly receiving
 attention by ecologists and evolutionary biologists, such studies need
 genetically homogeneous replicates-a challenging hurdle in outcrossing
 plants. This could potentially be overcome by using tissue culture
 techniques. However, plants regenerated from tissue culture may show
 aberrant phenotypes and "somaclonal" variation. Here we examined
 the somaclonal variation due to tissue culturing using the response to
 cold treatment of the photosynthetic efficiency (chlorophyll fluorescence
 measurements for Fv/Fm, Fv'/Fm' and ΦPSII, representing maximum
 efficiency of photosynthesis for dark- and light-adapted leaves, and the
 actual electron transport operating efficiency, respectively, which are
 reliable indicators of photoinhibition and damage to the photosynthetic
 electron transport system). We compared this to variation among
 half-sibling seedlings from three different families of Arabidopsis lyrata
 ssp. petraea. Somaclonal variation was limited and we could successfully
 detect within-family variation in change in chlorophyll fluorescence due
 to cold shock with the help of tissue-culture derived replicates.
 Icelandic and Norwegian families exhibited higher chlorophyll
 fluorescence, suggesting higher performance after cold shock, than a
 Swedish family. Although the main effect of tissue culture on Fv/Fm,
 Fv'/Fm' and ΦPSII, was small, there were significant
 interactions between tissue culture and family, suggesting that the effect
 of tissue culture is genotype-specific. Tissue-cultured plantlets were
 less affected by cold treatment than seedlings, but to a different extent
 in each family. These interactive effects, however, were comparable to, or
 much smaller than the single effect of family. These results suggest that
 tissue culture is a useful method for obtaining genetically homogenous
 replicates for studying genotype-environment interaction related to
 adaptively-relevant phenotypes, such as cold response, in non-model
 outcrossing plants.
description: Data for Alp clone cold
 reaction for Dryad.xlsxThe row data is in
 "data" sheet. The description of the data is in
 "read.me" sheet. The description of the data: 1 row indicates 1
 plant for which chlorophyll fluorescence was measured. Column
 "source". Which plants were derived from "seed" (i.e.
 seedling) or "clone" (i.e. tissule cultured plantlet). Column
 "w.genotype". If clone, name of genotype is provided. Otherwise,
 "half-sib". Column "pop". Name of populations. Either
 "ardal" in Norway, "notsand" in Sweden, or
 "sandfell" in Iceland. Column "treat". Experimental
 treatments. "pre": pre-cold treatment. "post":
 post-cold treatment. Column "ind". Number of plants. Column
 "block". Name of blocks. Chlorophyll fluorescence of plants in
 the same block was measured at the same time. Column "fvfm".
 Fv/Fm, chlorophyll fluorescence. Column "phy". Fv’/Fm’,
 chlorophyll fluorescence. Column "fvfm2". ФPSII, chlorophyll
 fluorescence.