DOI: 10.5061/dryad.pg4f4qrws
title: Two species of the Entoloma quadratum–murrayi complex from Japan, E. kermesinum sp. nov. and E. flavescens sp. nov.

description: We describe two new species, Entoloma. kermesinum sp. nov. and E.
 flavescens sp. nov., which are confused with E. quadratum and E. murrayi,
 respectively. We sequenced the large subunit of mitochondrial ribosomal
 RNA, the nuclear ribosomal internal transcribed spacer region and 22
 single-copy genes for 51 specimens of E. kermesinum, E. flavescens, E.
 album, and related species. Species boundaries were assessed using the
 molecular phylogenetics and population genetics approaches. Specimens of
 E. kermesinum, E. flavescens, and E. album formed independent clades,
 which were phylogenetically distinct from the specimens of E. quadratum
 and E. murrayi collected around the type locality (i.e., New England).
 Although the phylogenetic distance between E. flavescens and E. album was
 small, gene flow between them was restricted in areas where they
 coexisted, suggesting reproductive isolation. Therefore, these five
 species can be treated as independent species. We found characteristics
 useful for identifying E. kermesinum and E. flavescens. In particular, E.
 kermesinum is characterized by a crimson to brown-red and fibrillose
 pileus, finely covered by whitish fibrous scales; E. flavescens is
 characterized by a lemon-yellow to tan and shiny-to-silky pileus. In
 addition, relatively large basidiospores and clamp connections are
 diagnostic features of these two species.
description: # Two new species of the Entoloma quadratum-murrayi complex from Japan, E.
 kermesinum sp. nov. and E. flavescens sp. nov. Input and output data of
 phylogenetic analyses in "Two new species of the Entoloma
 quadratum-murrayi complex from Japan, E. kermesinum sp. nov. and E.
 flavescens sp. nov. ## DATA-SPECIFIC INFORMATION FOR INPUT FILES of
 MOLECULAR PHYLOGENETIC INFERENCE \[single_copy_gene.fasta] A multiple
 sequence alignment file (fasta format) of 21 single copy genes used for
 reconstructing ML tree using RAxML. Alignment data contain concatenated
 sequences of 21 single copy genes (7,432 bp).
 \[whole_AIC_partitioned.partition] A partition file for thelignment file
 ("single_copy_gene.fasta") used for reconstructing ML tree using
 RAxML \[MLtree_with_BS_single-copy-gene.nwk] ML tree with bootstrap values
 reconstructed from concatnated sequences of 21 single copy genes using
 RAxML (newick format) \[ITS.fasta] A multiple sequence alignment file
 (fasta format) of the nuclear ribosomal internal transcribed spacer (ITS)
 region used for reconstructing ML tree using RAxML. Alignment data contain
 concatenated sequences of ITS1 and ITS2 regions (727 bp).
 \[MLtree_with_BS_ITS.nwk] ML tree with bootstrap values reconstructed from
 concatnated sequences of ITS1 and ITS2 regions using RAxML (newick format)
 \[mtLSU.fasta] A multiple sequence alignment file (fasta format) of the
 large subunit of mitochondrial ribosomal RNA (mtLSU) used for
 reconstructing ML tree using RAxML. Alignment data contain concatenated
 sequences of mtLSU (282 bp). \[MLtree_with_BS_mtLSU.nwk] ML tree with
 bootstrap values reconstructed from mtLSU sequences using RAxML (newick
 format)