cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/r1qvq-eef65
title: Cos7 cells

description: looking for midbodies in Cos7 cells
description: <strong>Tilt Series Date:</strong> 2015-02-07</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Cos7</p>
<strong>Strain:</strong> Cos7 cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Sample Preparation:</strong> cells+ thymidine 2mM, 11 hour after release</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-02-07-12/rawdata/</p>cos7atlas20007.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-02-07-12/rawdata/cos7atlas20007.mrc"
        > <i class="download icon"></i> Download </a></p>

        cos7atlas20007.rec,  Reconstruction (Pixel Size 1.92 nm), 1.2 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.r4uxvj
title: Occurrence Download

description: A dataset containing 124 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lophophorus impejanus",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 124 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046971-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/m359f-3ds09
title: twitching Pseudomonas WT

description: Box YWC225Cells incubated on grid to promote twitching
description: <strong>Tilt Series Date:</strong> 2016-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 55.0°), step: 2.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sample prepared in collaboration with Alex Persat</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-23-7/3dimage_79382/</p>20160923_YWC225_10008_part62_4.rec,  Reconstruction (Pixel Size 1.28 nm), 134.2 MB  
        
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        > <i class="download icon"></i> Download </a></p>

        20160923_YWC225_10008.mrc,  Tilt Series (Pixel Size 0.32 nm), 441.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-23-7/rawdata/20160923_YWC225_10008.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/exy8d-4r131
title: Pseudomonas full length aer2

description: Pseudomonas full length aer2 on plasmid, induction with arabinose
description: <strong>Tilt Series Date:</strong> 2015-07-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-07-29-40/rawdata/</p>Aer2inducedJuly0034.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-07-29-40/rawdata/Aer2inducedJuly0034.mrc"
        > <i class="download icon"></i> Download </a></p>

        Aer2inducedJuly0034_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-07-29-40/3dimage_59859/Aer2inducedJuly0034_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a6u2eq
title: Occurrence Download

description: A dataset containing 21423 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is United States of America",
 "TaxonKey is Pontia protodice"
 ]
}

 The dataset includes 21423 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046970-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/5d9p7-bme21
title: M. xanthus PilY1.3-sfGFP

description: PilY1.3-sfGFP (SA7661+pMAT321)Grid box YWC262
description: <strong>Tilt Series Date:</strong> 2017-11-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-11-27-93/rawdata/</p>20171127_YWC262_10096.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-11-27-93/rawdata/20171127_YWC262_10096.mrc"
        > <i class="download icon"></i> Download </a></p>

        20171127_YWC262_10096_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9d7dc-18t11
title: twitching Pseudomonas delta-pilH

description: Box YWC213delta-pilH hyperpiliated mutantCells incubated on mucin-coated grid to promote twitching
description: <strong>Tilt Series Date:</strong> 2016-06-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sample prepared in collaboration with Alex Persat</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-06-01-10/rawdata/</p>20160531_YWC213_20011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-06-01-10/rawdata/20160531_YWC213_20011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160531_YWC213_20011_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-06-01-10/3dimage_75112/20160531_YWC213_20011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ytxv2-gpg57
title: H. pylori 26695 WT

description: grid box YWC259
description: <strong>Tilt Series Date:</strong> 2017-07-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-07-06-73/rawdata/</p>20170706_YWC259_10074.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-07-06-73/rawdata/20170706_YWC259_10074.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170706_YWC259_10074.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-07-06-73/3dimage_85792/20170706_YWC259_10074.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.c24z5z
title: Occurrence Download

description: A dataset listing the 24 species recorded in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-8.60639 41.15035,-8.60698 41.14699,-8.60243 41.14711,-8.60314 41.15032,-8.60639 41.15035))",
 "OccurrenceStatus is Present",
 "TaxonKey is Animalia",
 "Year 2020-2025"
 ]
}

 The dataset's 24 records were derived from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046943-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/6w27b-6v842
title: E.coli minicell/Pul T2SS

description: grid box YWC250strain PAP5359 (minCDE knockout)
description: <strong>Tilt Series Date:</strong> 2017-04-17</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain provided by Ingrid Guilvout in Olivera Francetic lab</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-04-17-7/rawdata/</p>20170417_YWC250_10007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-04-17-7/rawdata/20170417_YWC250_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170417_YWC250_10007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-04-17-7/3dimage_83876/20170417_YWC250_10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6t29k-m1s37
title: T9_ABK triple mutant

description: This is a sample that was prepared when Paul vsited
description: <strong>Tilt Series Date:</strong> 2017-01-25</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Porphyromonas gingivalis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Paul Veith and Eric Reynolds</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-25-29/rawdata/</p>sq80003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-25-29/rawdata/sq80003.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq80003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-25-29/3dimage_81533/sq80003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.5g6f4u
title: Occurrence Download

description: A dataset containing 588 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is United States of America",
 "TaxonKey is Pyrisitia nise"
 ]
}

 The dataset includes 588 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046974-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/2k74d-e1c47
title: Caulobacter crescentus ftsE del

description: Caulobacter crescentus, ftsE, FtsZ, Long cell, PYE
description: <strong>Tilt Series Date:</strong> 2017-01-17</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTOMO</p>
<strong>Collaborators and Roles:</strong> Goley ED at Johns Hopkins University School of Medicine</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-01-17-23/rawdata/</p>montage40005.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-01-17-23/rawdata/montage40005.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage40005.rec,  Reconstruction (Pixel Size 1.92 nm), 622.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2017-01-17-23/3dimage_81266/montage40005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.rewjvx
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Enteromius vandewallei)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046973-250811113504898/datasets/export for details.
DOI: 10.22002/w9c8f-whk76
title: HeLa cells

description: Synchronized HeLa cells, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2015-01-15</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Sample Preparation:</strong> Synchronized HeLa cells with siRNA NUP 153 and Thymidine 2mM</p>
  rra2015-01-15-56_slicer12712.jpg: Microtubule end
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-15-56/rawdata/</p>helayfp70001.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-15-56/rawdata/helayfp70001.mrc"
        > <i class="download icon"></i> Download </a></p>

        helayfp70001_full.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2015-01-15-56/3dimage_51119/helayfp70001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.28920987
title: Egyptian Consensus Guidance for Treatment of Adults with Non-Infectious Uveitis

description: Developing and setting up an Egyptian consensus for the management of NIU. A two-phase survey was implemented to obtain expert opinions from Egyptian specialists in the management of non-infectious uveitis. A core team of specialists in management of NIU formulated 21 clinical questions that were structured according to the PICO format (population, intervention, comparison, and the outcome). The literature review team conducted a systemic literature review to address the PICO questions. Based on literature search, a set of 21 sectioned recommendation statements were developed. The differences in ethnicity and socioeconomic standards of the Egyptian population raised the need to develop an Egyptian consensus guidance for management of NIU. The management statements developed in this work were based on a thorough review of the literature and consensus agreement of a national expert panel. Systemic immunosuppression should be initiated during steroid tapering and should be used as first line along with steroids in case of underlying systemic disease. The panel recommended that maintenance dose of steroids should not exceed 7.5 mg daily. A major limitation facing the Egyptian health system is the socioeconomic status and limited insurance coverage. Hence, the use of conventional immunosuppression is usually exhausted before considering biologic therapy, and the role of biosimilars is emphasized to their relatively lower cost, similar efficacy, and safety in comparison to the original molecule. This guideline provides direction for rheumatologists and ophthalmologists making decisions on management of adults with non-infectious uveitis. Putting into consideration that clinical practice varies based on local pharmaceutical availability and patient demographics.
DOI: 10.6084/m9.figshare.28920987.v1
title: Egyptian Consensus Guidance for Treatment of Adults with Non-Infectious Uveitis

description: Developing and setting up an Egyptian consensus for the management of NIU. A two-phase survey was implemented to obtain expert opinions from Egyptian specialists in the management of non-infectious uveitis. A core team of specialists in management of NIU formulated 21 clinical questions that were structured according to the PICO format (population, intervention, comparison, and the outcome). The literature review team conducted a systemic literature review to address the PICO questions. Based on literature search, a set of 21 sectioned recommendation statements were developed. The differences in ethnicity and socioeconomic standards of the Egyptian population raised the need to develop an Egyptian consensus guidance for management of NIU. The management statements developed in this work were based on a thorough review of the literature and consensus agreement of a national expert panel. Systemic immunosuppression should be initiated during steroid tapering and should be used as first line along with steroids in case of underlying systemic disease. The panel recommended that maintenance dose of steroids should not exceed 7.5 mg daily. A major limitation facing the Egyptian health system is the socioeconomic status and limited insurance coverage. Hence, the use of conventional immunosuppression is usually exhausted before considering biologic therapy, and the role of biosimilars is emphasized to their relatively lower cost, similar efficacy, and safety in comparison to the original molecule. This guideline provides direction for rheumatologists and ophthalmologists making decisions on management of adults with non-infectious uveitis. Putting into consideration that clinical practice varies based on local pharmaceutical availability and patient demographics.
DOI: 10.6084/m9.figshare.28941583
title: M2 Macrophages Mitigate Ocular Surface Inflammation and Promote Recovery in a Mouse Model of Dry Eye

description: Dry eye disease (DED) is a chronic, progressive, multifactorial condition characterized by tear film instability and ocular surface damage. Ocular surface inflammation is one of the main mechanisms of DED. This study aims to investigate the therapeutic effects of anti-inflammatory M2 macrophages on ocular surface inflammation and their potential mechanisms in improving dry eye symptoms in a mouse model. Mouse macrophages (RAW264.7) were polarized into M2 macrophages by IL-4 under different osmolarities, and M2 macrophage conditioned medium (M2-CM) was collected. Flow cytometry and ELISA were applied to measure the cytokine expression of the M2 macrophages. Primary mouse corneal epithelial cells (CECs) were co-cultured with RAW264.7 and M2 macrophages using a Transwell system. The viability and migration of CECs were assessed using CCK-8 and scratch assays. Mouse DED was established by subcutaneous injection of scopolamine, and the therapeutic effects of M2-CM were evaluated by phenol red thread test, fluorescein staining, and tear film breakup time (BUT). PCR and immunofluorescence staining were applied to observe inflammatory factors and cells on the ocular surface. M2 macrophages enhanced CEC viability, proliferation, and migration, but hyperosmolarity inhibited M2 macrophage polarization. In the DED model, M2-CM improved ocular surface conditions, reduced pro-inflammatory cytokine expression, and increased anti-inflammatory factors. Immunofluorescence revealed reduced pro-inflammatory cells (M1 macrophages, Th1, and Th17) and increased M2 macrophages in the ocular tissues after M2-CM treatment. These results suggest that M2-CM ameliorates ocular surface inflammation and promotes recovery in DED, offering a potential therapeutic strategy for DED.