cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5211445.v1
title: Electrochemical Determination of 6-thioguanine and Its Interaction with DNA Oligonucleotides Using Disposable Graphite Pencil Electrodes

description: Disposable graphite pencil electrodes were used in this study for the electrochemical determination of 6-thioguanine using its interaction with a single stranded Oligo (A)<sub>25</sub> composed of twenty-five adenine (A) bases or double stranded Oligo (A)<sub>25</sub>–Oligo (T)<sub>25</sub>, a hybrid form of Oligo (A)<sub>25</sub> and Oligo (T)<sub>25</sub> composed of twenty-five thymine (T) bases. The surface interaction was evaluated by monitoring the changes of the oxidation signals of 6-thioguanine and adenine. The electrochemical measurements were performed using differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy. The experimental conditions, including the concentration of 6-thioguanine and its period of interaction with single stranded Oligo (A)<sub>25</sub> or double stranded Oligo (A)<sub>25</sub>–Oligo (T)<sub>25</sub>, were optimized by observing the changes in the 6-thioguanine and adenine oxidation currents by differential pulse voltammetry. The detection limit was determined to be 0.24 µM across the useful linear concentration range from 1 to 4 µM 6-thioguanine. The reported single-use graphite sensor provides a cost effective, rapid, and sensitive detection approach for the electrochemical characterization of the interaction between DNA and 6-thioguanine.
DOI: 10.6084/m9.figshare.5209270
title: Supplementary Data for Hynes et al. (2016)

description: This data set contains sequences, sequence alignments and phylogenetic trees used in the bioinformatic analyses presented in:<br><pre>Hynes AP, Shakya M, Mercer RG, Grüll MP, Bown L, Davidson F, Steffen E, Matchem H, Peach ME, Berger T, Grebe K, Zhaxybayeva O, Lang AS. <i>Functional and Evolutionary Characterization of a Gene Transfer Agent's Multilocus "Genome".</i> Mol Biol Evol. <b>2016</b> Oct;<b>33(10)</b>:2530-43. Epub 2016 Jun 24.<br><br>doi: 10.1093/molbev/msw125. PubMed PMID: 27343288; <br>PubMed Central PMCID: PMC5026251.<br><br>The contents of four files:<br><br>1) <b>32core_genes.zip</b>: amino acid sequences of 32 gene families (in FASTA format) that were used to reconstruct Rhodobacterales phylogeny (shown in Figure 6 in the article.)<br>2) <b>rcc00171_tree.zip</b>: alignment (in FASTA format) and tree file (in NEWICK format) for the tree shown in Figure 7B.<br>3) <b>gtaNphage_teeNafa.zip</b>: alignments (in FASTA format) and tree files (in NEWICK format) for all RcGTA genes and their phage homologs (trees are shown in Figures 7A and S2.)<br>4) <b>RcGTA.zip</b>: amino acid sequences of genes in the RcGTA genome. These are also available in GenBank.<br></pre>
DOI: 10.6084/m9.figshare.5211451.v2
title: Biodiversity Measures to Summarize Antibiotic Resistance

description: R script for data analysis in the manuscript Biodiversity Measures to Summarize Antibiotic Resistance. The data set included here is an example data set as the original data set cannot be made available without request to the Public Health Agency of Canada. Hence the R script will run but will create different figures and give different statistical results compared to the original figure and results in the manuscript. <br>Please use R version (3.3.2; 2016-10-31).<br>
DOI: 10.6084/m9.figshare.5209270.v1
title: Supplementary Data for Hynes et al. (2016)

description: This data set contains sequences, sequence alignments and phylogenetic trees used in the bioinformatic analyses presented in:<br><pre>Hynes AP, Shakya M, Mercer RG, Grüll MP, Bown L, Davidson F, Steffen E, Matchem H, Peach ME, Berger T, Grebe K, Zhaxybayeva O, Lang AS. <i>Functional and Evolutionary Characterization of a Gene Transfer Agent's Multilocus "Genome".</i> Mol Biol Evol. <b>2016</b> Oct;<b>33(10)</b>:2530-43. Epub 2016 Jun 24.<br><br>doi: 10.1093/molbev/msw125. PubMed PMID: 27343288; <br>PubMed Central PMCID: PMC5026251.<br><br>The contents of four files:<br><br>1) <b>32core_genes.zip</b>: amino acid sequences of 32 gene families (in FASTA format) that were used to reconstruct Rhodobacterales phylogeny (shown in Figure 6 in the article.)<br>2) <b>rcc00171_tree.zip</b>: alignment (in FASTA format) and tree file (in NEWICK format) for the tree shown in Figure 7B.<br>3) <b>gtaNphage_teeNafa.zip</b>: alignments (in FASTA format) and tree files (in NEWICK format) for all RcGTA genes and their phage homologs (trees are shown in Figures 7A and S2.)<br>4) <b>RcGTA.zip</b>: amino acid sequences of genes in the RcGTA genome. These are also available in GenBank.<br></pre>
DOI: 10.6084/m9.figshare.5209165
title: Analysis of <i>MTNR1B</i> gene polymorphisms in relationship with <i>IRS2</i> gene variants, epicardial fat thickness, glucose homeostasis and cognitive performance in the elderly

description: Genome-wide association studies pinpointed common variants in or near the <i>MTNR1B</i> gene encoding MT2 melatonin receptor to be strongly associated with fasting glucose levels. <i>IRS2</i> gene polymorphisms impact insulin resistance and epicardial fat (EF) thickness, which in turn is correlated with visceral adiposity, cognitive ability and risk for metabolic plus cardiovascular disease. We aimed to discover the interactions between <i>MTNR1B</i> and <i>IRS2</i> gene polymorphisms, insulin sensitivity, EF thickness and cognitive performance in the elderly. In 60 subjects aged 60 years and older, we evaluated five single nucleotide polymorphisms (SNPs) within the <i>MTNR1B</i> locus (rs10830962, rs4753426, rs12804291, rs10830963, rs3781638), the Gly1057Asp variant of <i>IRS2</i> gene (rs1805097), biochemical parameters, cognitive performance by the Mini Mental State Examination (MMSE) and EF thickness by transthoracic echocardiography. We found that <i>MTNR1B</i> and <i>IRS2</i> gene variants impacted EF thickness, lipid profile and glucose homeostasis. <i>IRS2</i> but not <i>MTNR1B</i> variants impacted MMSE scores. In conclusion, <i>MTNR1B</i> SNPs interact with <i>IRS2</i> gene variant, correlate with the amount of epicardial adipose tissue and impact glucose homeostasis and lipid profile influencing cardiometabolic risk.
DOI: 10.6084/m9.figshare.5209159
title: ARNTL, CLOCK and PER3 polymorphisms – links with chronotype and affective dimensions

description: Recently, seven single nucleotide polymorphisms (SNPs) of ARNTL, TIM and PER3 genes were found associated with affective temperaments in bipolar disorder patients. This study aimed to test whether a) the same associations appear in a non-clinical sample; b) the SNPs are related to other affective dimensions; c) the SNPs underpin the associations between chronotype and affective temperaments/dimensions. Three hundred thirty-eight university students completed the Temperament Scale of Memphis, Pisa, Paris and San Diego Auto-questionnaire, the Centre for Epidemiological Studies Depression Scale, the Perceived Stress Scale, the General Health Questionnaire, the Seasonal Pattern Assessment Questionnaire and the Composite Scale of Morningness. Seven SNPs of the ARNTL, TIM and PER3 genes were genotyped. According to nominal significance, ARNTL rs7107287 was associated with a cyclothymic temperament, depressive and stress symptoms, general mental health and perceived negative impact of seasonality, while TIM rs10876890 was associated with a hyperthymic temperament, and the TIM rs2291738 was associated with chronotype. Different SNPs were related to chronotype and affective temperaments/dimensions, and therefore, they seem to not underpin relationships between chronotype and affective dysfunction, that is, in the present study, eveningness was related to dysthymic, cyclothymic and irritable temperaments, more symptoms of depression, stress, worse mental health and a negative impact of seasonality, while morningness was related to hyperthymic temperament. The SNPs associations need further replication given that they did not achieve Bonferroni criteria of significance accounting for the number of polymorphisms considered and tests conducted.
DOI: 10.6084/m9.figshare.5209165.v1
title: Analysis of <i>MTNR1B</i> gene polymorphisms in relationship with <i>IRS2</i> gene variants, epicardial fat thickness, glucose homeostasis and cognitive performance in the elderly

description: Genome-wide association studies pinpointed common variants in or near the <i>MTNR1B</i> gene encoding MT2 melatonin receptor to be strongly associated with fasting glucose levels. <i>IRS2</i> gene polymorphisms impact insulin resistance and epicardial fat (EF) thickness, which in turn is correlated with visceral adiposity, cognitive ability and risk for metabolic plus cardiovascular disease. We aimed to discover the interactions between <i>MTNR1B</i> and <i>IRS2</i> gene polymorphisms, insulin sensitivity, EF thickness and cognitive performance in the elderly. In 60 subjects aged 60 years and older, we evaluated five single nucleotide polymorphisms (SNPs) within the <i>MTNR1B</i> locus (rs10830962, rs4753426, rs12804291, rs10830963, rs3781638), the Gly1057Asp variant of <i>IRS2</i> gene (rs1805097), biochemical parameters, cognitive performance by the Mini Mental State Examination (MMSE) and EF thickness by transthoracic echocardiography. We found that <i>MTNR1B</i> and <i>IRS2</i> gene variants impacted EF thickness, lipid profile and glucose homeostasis. <i>IRS2</i> but not <i>MTNR1B</i> variants impacted MMSE scores. In conclusion, <i>MTNR1B</i> SNPs interact with <i>IRS2</i> gene variant, correlate with the amount of epicardial adipose tissue and impact glucose homeostasis and lipid profile influencing cardiometabolic risk.
DOI: 10.6084/m9.figshare.5209159.v1
title: ARNTL, CLOCK and PER3 polymorphisms – links with chronotype and affective dimensions

description: Recently, seven single nucleotide polymorphisms (SNPs) of ARNTL, TIM and PER3 genes were found associated with affective temperaments in bipolar disorder patients. This study aimed to test whether a) the same associations appear in a non-clinical sample; b) the SNPs are related to other affective dimensions; c) the SNPs underpin the associations between chronotype and affective temperaments/dimensions. Three hundred thirty-eight university students completed the Temperament Scale of Memphis, Pisa, Paris and San Diego Auto-questionnaire, the Centre for Epidemiological Studies Depression Scale, the Perceived Stress Scale, the General Health Questionnaire, the Seasonal Pattern Assessment Questionnaire and the Composite Scale of Morningness. Seven SNPs of the ARNTL, TIM and PER3 genes were genotyped. According to nominal significance, ARNTL rs7107287 was associated with a cyclothymic temperament, depressive and stress symptoms, general mental health and perceived negative impact of seasonality, while TIM rs10876890 was associated with a hyperthymic temperament, and the TIM rs2291738 was associated with chronotype. Different SNPs were related to chronotype and affective temperaments/dimensions, and therefore, they seem to not underpin relationships between chronotype and affective dysfunction, that is, in the present study, eveningness was related to dysthymic, cyclothymic and irritable temperaments, more symptoms of depression, stress, worse mental health and a negative impact of seasonality, while morningness was related to hyperthymic temperament. The SNPs associations need further replication given that they did not achieve Bonferroni criteria of significance accounting for the number of polymorphisms considered and tests conducted.
DOI: 10.6084/m9.figshare.5209087
title: Data set Arbuscular mycorrhizal symbiosis mitigates the negative effects of salinity on durum wheat

description: Data set article 'Arbuscular mycorrhizal symbiosis mitigates the negative effects of salinity on durum wheat'
DOI: 10.6084/m9.figshare.5209087.v1
title: Data set Arbuscular mycorrhizal symbiosis mitigates the negative effects of salinity on durum wheat

description: Data set article 'Arbuscular mycorrhizal symbiosis mitigates the negative effects of salinity on durum wheat'
DOI: 10.6084/m9.figshare.5209048
title: Network analysis of surgical innovation_PlosOne_data.csv

description: Authors' elaborations from ScopusMeasures of academic impact (citations, fraction of citations by 1 year), network-based virality (cascade size, structural depth, structural width) and level of evidence of robotic surgery articles published up to 2014 classified surgical procedures. Titles, scopus electronic ids (eid) and year of publication are also provided.
DOI: 10.6084/m9.figshare.5209048.v1
title: Network analysis of surgical innovation_PlosOne_data.csv

description: Authors' elaborations from ScopusMeasures of academic impact (citations, fraction of citations by 1 year), network-based virality (cascade size, structural depth, structural width) and level of evidence of robotic surgery articles published up to 2014 classified surgical procedures. Titles, scopus electronic ids (eid) and year of publication are also provided.
DOI: 10.6084/m9.figshare.5208970
title: Calibration and validation data for cropland classification over two sites in 2013

description: The data are for two sites located in Belgium (top left: 51.00°N, 4.50°E and bottom right: 49.60°N, 5.80°E) and South Africa (top left: 26.85°S, 24.55°E and bottom right: 30.74°S, 29.77°E).. They each cover an area of 60 km x 60 km and are dominated by cropland. For both sites, a 20-m multi-sensor time series consisting of SPOT-4 (Take 5) data and Landsat-8 data spanning from February to December 2013 were at hand. For Landsat, only reflectances in the green, red, near infra-red and short wave infrared wavelengths were considered. The SPOT-4 and Landsat-8 images were processed with the Multi-sensor Atmospheric Correction and Cloud Screening processor. The time series were gap filled using linear interpolation and three spectral-temporal features were extracted. These spectral-temporal features correspond to reflectance composites at the minimum and the maximum of normalized difference vegetation index as well as the mean reflectance over the season. Such features were shown to provide high discrimination power between cropland and non-cropland areas.<br>Ground truth observations from the corresponding growing season supplemented the satellite image time series. In Belgium, field polygons were sourced from the Land Parcel Identification System and non-cropland polygons were digitized based on very high resolution imagery. In South Africa, both cropland and non-cropland objects were both digitized based on very high resolution data. These reference data sets were evenly split at the polygon level into two independent sets to be used for calibration and validation, respectively.
DOI: 10.6084/m9.figshare.5208970.v1
title: Calibration and validation data for cropland classification over two sites in 2013

description: The data are for two sites located in Belgium (top left: 51.00°N, 4.50°E and bottom right: 49.60°N, 5.80°E) and South Africa (top left: 26.85°S, 24.55°E and bottom right: 30.74°S, 29.77°E).. They each cover an area of 60 km x 60 km and are dominated by cropland. For both sites, a 20-m multi-sensor time series consisting of SPOT-4 (Take 5) data and Landsat-8 data spanning from February to December 2013 were at hand. For Landsat, only reflectances in the green, red, near infra-red and short wave infrared wavelengths were considered. The SPOT-4 and Landsat-8 images were processed with the Multi-sensor Atmospheric Correction and Cloud Screening processor. The time series were gap filled using linear interpolation and three spectral-temporal features were extracted. These spectral-temporal features correspond to reflectance composites at the minimum and the maximum of normalized difference vegetation index as well as the mean reflectance over the season. Such features were shown to provide high discrimination power between cropland and non-cropland areas.<br>Ground truth observations from the corresponding growing season supplemented the satellite image time series. In Belgium, field polygons were sourced from the Land Parcel Identification System and non-cropland polygons were digitized based on very high resolution imagery. In South Africa, both cropland and non-cropland objects were both digitized based on very high resolution data. These reference data sets were evenly split at the polygon level into two independent sets to be used for calibration and validation, respectively.
DOI: 10.6084/m9.figshare.5208898
title: Funders List Open Access Monographs in Media Studies

description: This list is meant as a resource for researchers in film and media studies to help them find their way to open access funding at their national research council or institutional funding body.<br>The list has been developed for the project: www.oamediastudies.com
DOI: 10.6084/m9.figshare.5208898.v1
title: Funders List Open Access Monographs in Media Studies

description: This list is meant as a resource for researchers in film and media studies to help them find their way to open access funding at their national research council or institutional funding body.<br>The list has been developed for the project: www.oamediastudies.com
DOI: 10.6084/m9.figshare.5208886
title: Supplementary Material for: Population Pharmacokinetics of Diazoxide in Children with Hyperinsulinemic Hypoglycemia

description: <b><i>Background:</i></b> Diazoxide is the first-line treatment for pediatric hyperinsulinemic hypoglycemia (HI). This study aimed to elucidate the pharmacokinetics of diazoxide in children with HI. <b><i>Methods:</i></b> We obtained 81 blood samples from 22 children with HI. Measured serum diazoxide concentrations were used for population pharmacokinetic analysis. Patient factors influencing pharmacokinetics were estimated using nonlinear mixed-effects model analysis. Relationships between drug exposure and adverse drug reactions were also investigated. <b><i>Results:</i></b> Diazoxide disposition in the body was described by a 1-compartment model. Oral clearance (CL/F) and the volume of distribution were proportional to body weight (WT), as expressed by CL/F in males (liters/h) = 0.0358 + 0.00374 × WT (kg). CL/F in females was 39% greater than that in males. Steady-state concentrations of diazoxide were similar following twice- and 3 times-daily dosing when the total daily doses were comparable. A patient whose serum diazoxide concentration exceeded 100 μg/mL over a 4-month period developed hyperglycemia. No significant correlation was observed between severity of hirsutism and diazoxide concentration. <b><i>Conclusion:</i></b> We have proposed for the first time a population pharmacokinetic model for diazoxide in children with HI. The potential risk of diabetes mellitus and/or hyperglycemia increases when serum concentrations of diazoxide exceed 100 μg/mL.
DOI: 10.6084/m9.figshare.5208886.v1
title: Supplementary Material for: Population Pharmacokinetics of Diazoxide in Children with Hyperinsulinemic Hypoglycemia

description: <b><i>Background:</i></b> Diazoxide is the first-line treatment for pediatric hyperinsulinemic hypoglycemia (HI). This study aimed to elucidate the pharmacokinetics of diazoxide in children with HI. <b><i>Methods:</i></b> We obtained 81 blood samples from 22 children with HI. Measured serum diazoxide concentrations were used for population pharmacokinetic analysis. Patient factors influencing pharmacokinetics were estimated using nonlinear mixed-effects model analysis. Relationships between drug exposure and adverse drug reactions were also investigated. <b><i>Results:</i></b> Diazoxide disposition in the body was described by a 1-compartment model. Oral clearance (CL/F) and the volume of distribution were proportional to body weight (WT), as expressed by CL/F in males (liters/h) = 0.0358 + 0.00374 × WT (kg). CL/F in females was 39% greater than that in males. Steady-state concentrations of diazoxide were similar following twice- and 3 times-daily dosing when the total daily doses were comparable. A patient whose serum diazoxide concentration exceeded 100 μg/mL over a 4-month period developed hyperglycemia. No significant correlation was observed between severity of hirsutism and diazoxide concentration. <b><i>Conclusion:</i></b> We have proposed for the first time a population pharmacokinetic model for diazoxide in children with HI. The potential risk of diabetes mellitus and/or hyperglycemia increases when serum concentrations of diazoxide exceed 100 μg/mL.
DOI: 10.6084/m9.figshare.5208853
title: The pollen of <i>Solanum</i> L. and its systematic significance

description: The morphology of 75 different pollen grains from 67 species of genus <i>Solanum</i> L., two species of genus <i>Cyphomandra</i> Sendt., and six species of genus <i>Lycianthes</i> (Dunal) Hassl. was studied using light microscopy and scanning electron microscopy. Among them, the pollen of 66 species is described for the first time. Our results suggest that the sexine ornamention of <i>Solanum</i>, <i>Cyphomandra</i> and <i>Lycianthes</i> is extremely similar, but the apertures of genus <i>Solanum</i> and <i>Cyphomandra</i> are not syncolpate while those of genus <i>Lycianthes</i> are syncolpate. Overall, we argued that the <i>Solanum</i> species studied demonstrated sufficient pollinic heterogeneity in their shapes, aperture feature and sexine ornamentation to enable their palynological characterisation and support the point of view that the genus <i>Cyphomandra</i> should be merged into <i>Solanum</i>, but <i>Lycianthes</i> should be an independent genus from the aspect of palynology.
DOI: 10.6084/m9.figshare.5208853.v2
title: The pollen of <i>Solanum</i> L. and its systematic significance

description: The morphology of 75 different pollen grains from 67 species of genus <i>Solanum</i> L., two species of genus <i>Cyphomandra</i> Sendt., and six species of genus <i>Lycianthes</i> (Dunal) Hassl. was studied using light microscopy and scanning electron microscopy. Among them, the pollen of 66 species is described for the first time. Our results suggest that the sexine ornamention of <i>Solanum</i>, <i>Cyphomandra</i> and <i>Lycianthes</i> is extremely similar, but the apertures of genus <i>Solanum</i> and <i>Cyphomandra</i> are not syncolpate while those of genus <i>Lycianthes</i> are syncolpate. Overall, we argued that the <i>Solanum</i> species studied demonstrated sufficient pollinic heterogeneity in their shapes, aperture feature and sexine ornamentation to enable their palynological characterisation and support the point of view that the genus <i>Cyphomandra</i> should be merged into <i>Solanum</i>, but <i>Lycianthes</i> should be an independent genus from the aspect of palynology.
DOI: 10.17028/rd.lboro.5208826
title: Data used in 'Investigation of polymer electrolyte membrane fuel cell internal behavior during long term operation and its use in prognostics'

description: This data-set has been used in the above paper (Investigation of polymer electrolyte membrane fuel cell internal behavior during long term operation and its use in prognostics) to investigate the variation of fuel cell parameters with the equations in the paper.<br>The deposit consists of a zip folder which can be extracted using 7-zip. The data files are in .csv format.