DOI: 10.5061/dryad.8w9ghx40n
title: Data from: Larvicidal potential of Pseudomonas mosselii isolated from Aedes (Diptera: Culicidae) egg surfaces against dengue vector mosquitoes and its impact on non-target organisms

description: The critical impact of mosquito-borne diseases on public health, along
 with the failure of current control strategies, highlights the need for
 novel mosquito control approaches, especially in tropical countries. This
 study explored the larvicidal properties of bacterial isolates from the
 egg surfaces of Aedes aegypti L. and Aedes albopictus Skuse (Diptera:
 Culicidae), the primary and secondary dengue vector mosquitoes in Sri
 Lanka. Among 30 bacterial isolates, a local strain of Pseudomonas mosselii
 Quesnel, isolated from the egg surfaces of dengue vectors, demonstrated
 strong larvicidal activity against Ae. aegypti. Larvicidal assays using
 the bacterial supernatant (OD 1.7) and freeze-dried products were
 performed across concentrations ranging from 80 to 2.1 mg/mL. The LC50
 values for the supernatant were 55, 54, and 52 mg/mL at 24, 48, and 72 h,
 respectively, with corresponding LC90 values of 91, 89, and 86 mg/mL.
 Freeze-dried supernatant showed LC50 values of 2.73, 2.64, and 2.63 mg/mL,
 while the freeze-dried bacterial culture showed higher toxicity with LC50
 values of 2.57, 2.51, and 2.44 mg/mL. Strong larvicidal activity was also
 observed against Culex quinquefasciatus Say (Diptera: Culicidae), a
 primary vector of filariasis. The freeze-dried products retained their
 efficacy for up to two months. Ovicidal and adulticidal assays indicated
 no significant effects on mosquito eggs or adults. However, the active
 components of bacterial metabolites significantly impacted the development
 of Aedes larvae. Toxicity effects were also observed in non-target
 organisms, including zooplankton and tadpoles. These findings suggest that
 the local Ps. mosselii strain may serve as a potential bio-larvicide
 against dengue vectors while underscoring the need for further
 environmental assessment.
description: # Data from: Larvicidal potential of Pseudomonas mosselii isolated from
 Aedes (Diptera: Culicidae) egg surfaces against dengue vector mosquitoes
 and its impact on non-target organisms Dataset DOI:
 [10.5061/dryad.8w9ghx40n](10.5061/dryad.8w9ghx40n) ## Description of the
 data and file structure This dataset collection presents the raw
 experimental data underlying laboratory bioassays that evaluated the
 mosquitocidal potential of Pseudomonas mosselii against dengue vector
 mosquitoes (Aedes aegypti and Aedes albopictus), as well as its effects on
 two non-target aquatic organisms (Culex quinquefasciatus and ostracods).
 The study assessed three formulations of the bacterial treatment, namely,
 cell-free supernatant (CFS), freeze-dried cell-free supernatant (FDCFS),
 and freeze-dried bacterial culture (FDBC), with treatment concentrations
 expressed in mg/mL. Each test concentration was replicated four times,
 along with four distilled water controls. Each dataset is provided in .csv
 format. * Dataset 1 reports larval mortality of *Aedes aegypti* exposed to
 different concentrations of CFS, FDCFS, and FDBC over 24, 48, and 72
 hours. Each treatment group involved 15 larvae per replicate. - Dataset 2
 presents the number of *Aedes aegypti* larvae reaching pupation and adult
 emergence after treatment exposure, with initial sample sizes of 15 larvae
 per concentration. * Dataset 3 includes time-to-event data (days to
 pupation and adult emergence) for *Aedes aegypti* larvae hatched from eggs
 and continuously exposed to CFS, FDCFS, or FDBC. Twenty individuals were
 exposed per treatment condition. - Dataset 4 mirrors Dataset 3 but focuses
 on *Aedes albopictus* larvae. It records the number of pupae and adults
 emerged, along with the development time. Each treatment condition had 20
 exposed individuals. * Dataset 5 contains mortality data for *Culex
 quinquefasciatus* larvae exposed to *Pseudomonas mosselii* treatments
 (CFS, FDCFS, FDBC) at various concentrations, with 15 larvae per treatment
 group. - Dataset 6 records the mortality of non-target ostracods after
 exposure to FDCFS and FDBC, with 20 organisms per treatment group. *
 Dataset 7 evaluates the long-term stability of the freeze-dried bacterial
 products (FDCFS and FDBC) by measuring larval mortality of *Aedes aegypti*
 at different storage intervals (immediate, 1 month, 2 months, and 3 months
 post-lyophilization). Each treatment replicate involved 15 larvae. All
 experiments were conducted under laboratory conditions with ethical
 approval obtained from the  Ethical Review Committee of Postgraduate
 Institute of Science, University of Peradeniya, Sri Lanka (Certificate ID:
 CEC_PGIS_2024_12). ### Files and variables #### File:
 Dataset_1_RawData_Larval_Mortality-Aedes.csv **Description:** This dataset
 records the number of dead Aedes aegypti larvae at different treatment
 concentrations of the cell-free supernatant (CFS), freeze-dried cell-free
 supernatant (FDCFS), and freeze-dried bacterial culture (FDBC) of
 Pseudomonas mosselii. Mortality data were collected at 24, 48, and 72
 hours post-exposure. Each concentration was tested on 15 larvae per
 replicate, with four replicates per concentration and four parallel
 control replicates using distilled water. The treatment concentrations are
 expressed in milligrams per milliliter (mg/mL). This dataset represents
 acute toxicity data used to determine dose-response effects over time.
 ##### Variables * Direct supernatant -CFS: * Freeze dried supernatant -
 FDCFS: * Freeze dried culture- FDBC: #### File:
 Dataset_2_RawData_Aedes_Devlopment.csv **Description:** This dataset
 presents the number of Aedes aegypti larvae that successfully reached the
 pupal and adult stages after exposure to Pseudomonas mosselii treatments
 of larval bioassays. The three formulations tested were cell-free
 supernatant (CFS), freeze-dried cell-free supernatant (FDCFS), and
 freeze-dried bacterial culture (FDBC). Each concentration was tested with
 15 larvae per replicate, using four replicates per treatment and four
 distilled water controls. The data capture potential sublethal effects by
 measuring how each treatment impacts development into later life stages.
 NA means-No observations recorded ##### Variables * Direct supernatant-
 CFS: * Freeze dried Supernatant -FDCFS: * Freeze dried bacterial culture-
 FDBC: #### File:
 Dataset_4_RawData-Ovicidal_and_Developmental-Albopictus.csv
 **Description:** Similar to Dataset 3, this dataset evaluates ovicidal and
 post-hatching developmental effects, but in Aedes albopictus. Eggs were
 exposed to Pseudomonas mosselii treatments, CFS, FDCFS, and FDBC and data
 were collected on the number of individuals reaching pupation and adult
 emergence, along with the number of days required to reach these stages.
 Each treatment was tested using 20 exposed eggs per replicate, with four
 replicates per concentration and four distilled water controls. The
 dataset allows for species-level comparison of developmental sensitivity
 to microbial treatment. NA means-No observations recorded ##### Variables
 * Direct supernatant- CFS: * Freeze dried Supernatant -FDCFS: * Freeze
 dried bacterial culture- FDBC: #### File:
 Dataset_3_RawData-Ovicidal_and_Development-_Aegypti.csv
 **Description:** This dataset contains the results from ovicidal bioassays
 performed on Aedes aegypti. Eggs were exposed to the three treatment
 formulations of Pseudomonas mosselii, cell-free supernatant (CFS),
 freeze-dried cell-free supernatant (FDCFS), and freeze-dried bacterial
 culture (FDBC) and development of hatched larvae was monitored until adult
 emergence. The dataset includes the number of larvae that reached pupation
 and adulthood, as well as the time taken to reach these developmental
 stages, recorded in days. Twenty mosquito eggs were exposed per treatment,
 with four replicates per treatment and four controls. These data help
 assess whether exposure at the egg stage delays or suppresses mosquito
 development. NA means-No observations recorded ##### Variables * Direct
 supernatant- CFS: * Freeze dried Supernatant -FDCFS: * Freeze dried
 bacterial culture- FDBC: #### File:
 Dataset_5_RawData-Culex_larval_mortality.csv **Description:** This dataset
 includes larval mortality data for Culex quinquefasciatus, a non-target
 mosquito species, exposed to the cell-free supernatant (CFS), freeze-dried
 cell-free supernatant (FDCFS), and freeze-dried bacterial culture (FDBC)
 of Pseudomonas mosselii. Mortality was recorded at 24, 48, and 72 hours
 post-exposure. Each concentration was tested using 15 larvae per
 replicate, with four replicates per treatment and four controls. The
 dataset is essential for evaluating the non-target specificity and
 environmental safety of the tested biolarvicides. ##### Variables * Direct
 supernatant -CFS: * Freeze dried supernatant - FDCFS: * Freeze dried
 culture- FDBC: #### File: Dataset_6_RawData_Non_target.csv
 **Description:** This dataset documents mortality in ostracods (small
 aquatic crustaceans) exposed to the freeze-dried products of Pseudomonas
 mosselii, specifically, FDCFS and FDBC. Each treatment was tested with 15
 ostracods per replicate, across four replicates per concentration and four
 controls. Mortality was recorded at 24 and 48 hours after exposure. These
 data contribute to the environmental risk assessment of microbial
 larvicides on aquatic microfauna. ##### Variables * Freeze dried cell free
 supernatant-FDCFS: * Freeze dried culture-FDBC: #### File:
 Dataset_7_RawData_Shelf_life.csv **Description:** This dataset evaluates
 the long-lasting larvicidal effects of the freeze-dried cell-free
 supernatant (FDCFS) and freeze-dried bacterial culture (FDBC) of
 Pseudomonas mosselii. Aedes aegypti larvae (15 per replicate) were exposed
 to the LC₅₀ concentration previously determined in Dataset 1. The
 treatments were stored at 4 °C and tested at four time intervals:
 immediately after lyophilization (0 months), and after 1, 2, and 3 months
 of storage. Mortality was assessed at 24, 48, and 72 hours post-exposure.
 Each time point included four replicates per treatment and four control
 replicates. This dataset reflects the storage stability and residual
 bioactivity of microbial larvicidal products. Merged cells in spreadsheets
 group treatment identifiers and replicate sets ##### Variables * Freeze
 dried cell free supernatant -FDCFS: * Freeze dried cell free supernatant
 -FDCFS: ## Access information Other publicly accessible locations of the
 data: * No Data was derived from the following sources: * No