cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7185836
title: Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate

description: ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P&gt; 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.
DOI: 10.7284/121074
title: Radiometer data collected during research cruise TN318 using a Eppley RAD instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185836.v1
title: Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate

description: ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P&gt; 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.
DOI: 10.7284/121242
title: Speedlog data collected during research cruise TN318 using a JRC JLN-550 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185827
title: Catheterization of the renal pelvis guided by cistoscopy in mares

description: ABSTRACT The objective of this study was to standardize the technique of access to the renal pelvis by means of ureteral catheterization guided by cystoscopy in mares. Eight animals of different races were used, with an average weight of 439kg. The mares were sedated and contained in containment trunk for the accomplishment of cystoscopy with flexible endoscope. After the identification of the left ureteral ostium, from where inside a catheter was introduced, which went through the entire extension of the ureter up to the renal pelvis. After identification of the left ureteral ostium, from where inside a catheter was introduced, into its lumen until reaching the renal pelvis. The location of the probe in the kidney was confirmed by transcutaneous ultrasonography. Three ml of urine was aspirated aseptically for cytology and microbiological culture. All the samples obtained negative results in the culture and sedimentation. None of the animals had any complications after catheterization. This study demonstrated that the collection of urine directly from the renal pelvis in mares, with the assistance of cystoscopy in the realization of the ureteral catheter, consists of a viable and safe procedure.
DOI: 10.6084/m9.figshare.7185824
title: Peritoneal inflammatory response of the donkeys (Equus asinus) submitted ovariectomy by laparotomy and laparoscopy

description: ABSTRACT The aim of this study was to evaluate the inflammatory response and protein concentrations in the peritoneal fluid of donkeys submitted to laparotomy and laparoscopic ovariectomy. Eighteen adult females, weighing on average 100kg, were divided into two groups, of 09 animals each, where in group I, they were ovariectomized by laparotomy and group II by laparoscopy. Following the surgeries, the following variables were evaluated in the peritoneal fluid: pH, density, number of red cells, leukocytes and acute phase proteins (fibrinogen, haptoglobin, albumin, antitrypsin, alpha-1 acid glycoprotein, ceruloplasmin, transferrin, Immunoglobulins A and G). All variables were analyzed before the surgeries, 12, 24, 48, 72 hours, 8 and 16 days after the surgical procedures. The number of red cells and leukocytes increased 24 hours after the surgeries in both groups. Haptoglobin, alpha-1 acid glycoprotein, albumin, transferrin, gammaglobulins A and G, and total proteins increased in both groups. Concentrations of ceruloplasmin did not change while antitrypsin was not detected in the asinine peritoneal fluid. A protein of 23,000kD, which has no name or description in the peritoneal liquid of donkeys, has been identified.
DOI: 10.6084/m9.figshare.7185827.v1
title: Catheterization of the renal pelvis guided by cistoscopy in mares

description: ABSTRACT The objective of this study was to standardize the technique of access to the renal pelvis by means of ureteral catheterization guided by cystoscopy in mares. Eight animals of different races were used, with an average weight of 439kg. The mares were sedated and contained in containment trunk for the accomplishment of cystoscopy with flexible endoscope. After the identification of the left ureteral ostium, from where inside a catheter was introduced, which went through the entire extension of the ureter up to the renal pelvis. After identification of the left ureteral ostium, from where inside a catheter was introduced, into its lumen until reaching the renal pelvis. The location of the probe in the kidney was confirmed by transcutaneous ultrasonography. Three ml of urine was aspirated aseptically for cytology and microbiological culture. All the samples obtained negative results in the culture and sedimentation. None of the animals had any complications after catheterization. This study demonstrated that the collection of urine directly from the renal pelvis in mares, with the assistance of cystoscopy in the realization of the ureteral catheter, consists of a viable and safe procedure.
DOI: 10.7284/121240
title: SSV (Sound Speed Velocimeter) data collected during research cruise TN318 using a AML SV&T instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185824.v1
title: Peritoneal inflammatory response of the donkeys (Equus asinus) submitted ovariectomy by laparotomy and laparoscopy

description: ABSTRACT The aim of this study was to evaluate the inflammatory response and protein concentrations in the peritoneal fluid of donkeys submitted to laparotomy and laparoscopic ovariectomy. Eighteen adult females, weighing on average 100kg, were divided into two groups, of 09 animals each, where in group I, they were ovariectomized by laparotomy and group II by laparoscopy. Following the surgeries, the following variables were evaluated in the peritoneal fluid: pH, density, number of red cells, leukocytes and acute phase proteins (fibrinogen, haptoglobin, albumin, antitrypsin, alpha-1 acid glycoprotein, ceruloplasmin, transferrin, Immunoglobulins A and G). All variables were analyzed before the surgeries, 12, 24, 48, 72 hours, 8 and 16 days after the surgical procedures. The number of red cells and leukocytes increased 24 hours after the surgeries in both groups. Haptoglobin, alpha-1 acid glycoprotein, albumin, transferrin, gammaglobulins A and G, and total proteins increased in both groups. Concentrations of ceruloplasmin did not change while antitrypsin was not detected in the asinine peritoneal fluid. A protein of 23,000kD, which has no name or description in the peritoneal liquid of donkeys, has been identified.
DOI: 10.7284/121241
title: TSG (Thermosalinograph) data collected during research cruise TN318 using a Sea-Bird SBE-21 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.7284/119090
title: GNSS (Global Navigation Satellite System) data collected during research cruise TN319 using a C&C C-Nav 3050 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185812
title: Knowledge and attitudes of official inspectors at slaughterhouses in southern Brazil regarding animal welfare

description: ABSTRACT We assessed the views, knowledge, and attitudes of livestock inspectors working in federally inspected slaughterhouses in southern Brazil, the major pig and poultry processors in the country, regarding farm animal behavior and welfare, and factors that may influence animal welfare improvements at abattoirs. An online questionnaire with closed and open questions was sent to all the federally inspected slaughterhouses in the three states of the region. All respondents (n = 83) strongly agreed that animals are capable of feeling pain, but fewer agreed that animals have mental states and individual temperament, or that animals can feel boredom or frustration. In the assessment of respondents, professionals in Brazil receive insufficient training in animal welfare; most believe that stockpeople do not understand the relationship between animal welfare and meat quality. Furthermore, most respondents believe that stockpeople are not satisfied with their working conditions at slaughterhouses and are not motivated to adopt practices to improve animal welfare. Respondents reported positive attitudes towards animal welfare but insufficient understanding and training on animal welfare during professional education. The three main actions that, according to the respondents, are required to successfully implement humane slaughter in Brazil are better selection and greater valorization of the stockpeople by the industry; refinements of the legislation to indicate minimum and maximum standards for specific species, practices, and outcomes; and introduction of animal welfare as a topic during the professional training of professionals that will oversee and support the implementation of humane practices in slaughterhouses.
DOI: 10.6084/m9.figshare.7185812.v1
title: Knowledge and attitudes of official inspectors at slaughterhouses in southern Brazil regarding animal welfare

description: ABSTRACT We assessed the views, knowledge, and attitudes of livestock inspectors working in federally inspected slaughterhouses in southern Brazil, the major pig and poultry processors in the country, regarding farm animal behavior and welfare, and factors that may influence animal welfare improvements at abattoirs. An online questionnaire with closed and open questions was sent to all the federally inspected slaughterhouses in the three states of the region. All respondents (n = 83) strongly agreed that animals are capable of feeling pain, but fewer agreed that animals have mental states and individual temperament, or that animals can feel boredom or frustration. In the assessment of respondents, professionals in Brazil receive insufficient training in animal welfare; most believe that stockpeople do not understand the relationship between animal welfare and meat quality. Furthermore, most respondents believe that stockpeople are not satisfied with their working conditions at slaughterhouses and are not motivated to adopt practices to improve animal welfare. Respondents reported positive attitudes towards animal welfare but insufficient understanding and training on animal welfare during professional education. The three main actions that, according to the respondents, are required to successfully implement humane slaughter in Brazil are better selection and greater valorization of the stockpeople by the industry; refinements of the legislation to indicate minimum and maximum standards for specific species, practices, and outcomes; and introduction of animal welfare as a topic during the professional training of professionals that will oversee and support the implementation of humane practices in slaughterhouses.
DOI: 10.7284/121243
title: GNSS (Global Navigation Satellite System) data collected during research cruise TN319 using a C&C C-Nav 2000 instrument system onboard the platform RV Thomas G. Thompson
DOI: 10.6084/m9.figshare.7185800
title: Individual study of anthropometric variation, energy and macronutrients intakes in Paralympic Track and Field athletes in different phases of the season

description: Abstract Aims: a) to measure individual variations in body mass (BM), sum of skinfolds (SSF) and energy intake of Paralympic track and field athletes b) to evaluate whether individual consumption of macronutrients meets recommended levels in three distinct periods of training. Methods: Ten Paralympic track and field athletes were evaluated during three periods: 1) end of season, 2) after vacation, and 3) preparation for the Paralympic Games London 2012. Food history and the 24-hour Dietary Recall methods provided information on daily food intake. To assess changes in body composition, we measured skinfolds and BM. Descriptive statistics were utilized to describe individual results. Results: BM varied among athletes and phases, but most (n=8) had higher SSF after vacation. Four athletes reported an increase in energy intake of more than 500 kcal during their vacation period, while eight athletes reported maintaining their intake in the preparatory phase for the competition. Carbohydrate intake was adequate for most athletes in the end of season period and in the preparatory phase for competition, and most athletes had reduced lipid intake after vacation and in the preparatory phase. Conclusion: We observed important variations in BM and SSF, in food intake and macronutrients between the three evaluated periods. The nutritional status of the athletes suggests that inadequacies that might affect the performance of sprinters and middle-distance runners are more likely to occur during the preparatory period before competitions.
DOI: 10.6084/m9.figshare.7185803
title: People living with AIDS: Association between nursing diagnoses and sociodemographic/clinical characteristics

description: ABSTRACT Objective: To analyze the association between nursing diagnoses and sociodemographic and clinical characteristics in people living with AIDS. Method: Cross-sectional study with 100 people living with AIDS in a University Hospital. Data collection took place between January and July 2015, using an interview script and physical examination. The association occurred through Pearson's Chi-Square Test and Fisher's Exact Test. Results: The most prevalent nursing diagnoses were: ineffective protection, poor knowledge, lack of adherence and sexual dysfunction. Significant associations were observed among nursing diagnoses with the following sociodemographic and clinical characteristics: marital status, place of residence, level of schooling, family income, modes of transmission of acquired immunodeficiency virus, current opportunistic infection, abandonment of treatment, difficulty of access to health services and use of alcohol and illicit drugs. Conclusion: The diagnoses presented significant associations with sociodemographic and clinical aspects.
DOI: 10.6084/m9.figshare.7185800.v1
title: Individual study of anthropometric variation, energy and macronutrients intakes in Paralympic Track and Field athletes in different phases of the season

description: Abstract Aims: a) to measure individual variations in body mass (BM), sum of skinfolds (SSF) and energy intake of Paralympic track and field athletes b) to evaluate whether individual consumption of macronutrients meets recommended levels in three distinct periods of training. Methods: Ten Paralympic track and field athletes were evaluated during three periods: 1) end of season, 2) after vacation, and 3) preparation for the Paralympic Games London 2012. Food history and the 24-hour Dietary Recall methods provided information on daily food intake. To assess changes in body composition, we measured skinfolds and BM. Descriptive statistics were utilized to describe individual results. Results: BM varied among athletes and phases, but most (n=8) had higher SSF after vacation. Four athletes reported an increase in energy intake of more than 500 kcal during their vacation period, while eight athletes reported maintaining their intake in the preparatory phase for the competition. Carbohydrate intake was adequate for most athletes in the end of season period and in the preparatory phase for competition, and most athletes had reduced lipid intake after vacation and in the preparatory phase. Conclusion: We observed important variations in BM and SSF, in food intake and macronutrients between the three evaluated periods. The nutritional status of the athletes suggests that inadequacies that might affect the performance of sprinters and middle-distance runners are more likely to occur during the preparatory period before competitions.
DOI: 10.6084/m9.figshare.7185803.v1
title: People living with AIDS: Association between nursing diagnoses and sociodemographic/clinical characteristics

description: ABSTRACT Objective: To analyze the association between nursing diagnoses and sociodemographic and clinical characteristics in people living with AIDS. Method: Cross-sectional study with 100 people living with AIDS in a University Hospital. Data collection took place between January and July 2015, using an interview script and physical examination. The association occurred through Pearson's Chi-Square Test and Fisher's Exact Test. Results: The most prevalent nursing diagnoses were: ineffective protection, poor knowledge, lack of adherence and sexual dysfunction. Significant associations were observed among nursing diagnoses with the following sociodemographic and clinical characteristics: marital status, place of residence, level of schooling, family income, modes of transmission of acquired immunodeficiency virus, current opportunistic infection, abandonment of treatment, difficulty of access to health services and use of alcohol and illicit drugs. Conclusion: The diagnoses presented significant associations with sociodemographic and clinical aspects.
DOI: 10.7284/121247
title: Speedlog data collected during research cruise TN319 using a JRC JLN-550 instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185797
title: Movement patterns of humpback whales (Megaptera novaeangliae) reoccupying a Brazilian breeding ground

description: Abstract: The population of humpback whales from breeding stock A is increasing, and little is known about the routes used by humpbacks that move north of the main calving area of Brazil, the Abrolhos Bank. The aim of this study was to describe the movements of humpback whales in a reoccupation wintering area (Serra Grande, Bahia state, Brazil) based on land-based surveys to test if movement patterns change during the season and between years, due to group composition, behavioral state, and distance to the coast. The mean leg speed of the groups sighted was 6.88 (±2.92) km/h, and leg speed was positively correlated with distance to the coast. There was an increase in leg speed and distance to the coast with increasing number of escorts in the groups with calves. The mean linearity value for group trajectory was 0.81 (±0.19) and the mean reorientation rate was 25.72 (±19.09) º/min. We observed a predominance of trajectories heading south throughout the study. Groups exhibiting more erratic movements early in the season, and groups moving south showed more linear trajectories than groups moving north, indicating the beginning of their migration back to the feeding grounds. Energy conserving strategies and social context affect the movements of humpback whales in Serra Grande, resulting in the observed patterns of the reoccupation of available and suitable habitat north of Abrolhos. Thereby, special attention should be given managing activities with the potential to disturb or displace whales using the region to calve and breed.
DOI: 10.7284/119091
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise TN320 using a Hawaii UHDAS instrument system onboard the platform RV Thomas G. Thompson

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7185797.v1
title: Movement patterns of humpback whales (Megaptera novaeangliae) reoccupying a Brazilian breeding ground

description: Abstract: The population of humpback whales from breeding stock A is increasing, and little is known about the routes used by humpbacks that move north of the main calving area of Brazil, the Abrolhos Bank. The aim of this study was to describe the movements of humpback whales in a reoccupation wintering area (Serra Grande, Bahia state, Brazil) based on land-based surveys to test if movement patterns change during the season and between years, due to group composition, behavioral state, and distance to the coast. The mean leg speed of the groups sighted was 6.88 (±2.92) km/h, and leg speed was positively correlated with distance to the coast. There was an increase in leg speed and distance to the coast with increasing number of escorts in the groups with calves. The mean linearity value for group trajectory was 0.81 (±0.19) and the mean reorientation rate was 25.72 (±19.09) º/min. We observed a predominance of trajectories heading south throughout the study. Groups exhibiting more erratic movements early in the season, and groups moving south showed more linear trajectories than groups moving north, indicating the beginning of their migration back to the feeding grounds. Energy conserving strategies and social context affect the movements of humpback whales in Serra Grande, resulting in the observed patterns of the reoccupation of available and suitable habitat north of Abrolhos. Thereby, special attention should be given managing activities with the potential to disturb or displace whales using the region to calve and breed.