cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.1017218
title: N2 Schafer Lab N2 (Bristol, Uk) | 2011-03-15T11:33:34+00:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=QukKwRQhVeU
<b>strain</b> : N2
<b>timestamp</b> : 2011-03-15T11:33:34+00:00
<b>gene</b> : -N/A-
<b>chromosome</b> : -N/A-
<b>allele</b> : -N/A-
<b>strain_description</b> : Schafer Lab N2 (Bristol, UK)
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : N2 on food R_2011_03_15__11_33_34___7___1
<b>total time (s)</b> : 899.0
<b>frames per second</b> : 30.03
<b>video micrometers per pixel</b> : 4.2072
<b>number of segmented skeletons</b> : 26665
DOI: 10.5281/zenodo.1017864
title: Ju258 C. Elegans Wild Isolate (Ribeiro Frio, Madeira) | 2011-03-29T12:52:40+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=zivrckPJ6eQ
<b>strain</b> : JU258
<b>timestamp</b> : 2011-03-29T12:52:40+01:00
<b>gene</b> : -N/A-
<b>chromosome</b> : -N/A-
<b>allele</b> : -N/A-
<b>strain_description</b> : C. elegans Wild Isolate (Ribeiro Frio, Madeira)
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : 798 JU258 on food L_2011_03_29__12_52_40___7___7
<b>total time (s)</b> : 899.0
<b>frames per second</b> : 30.03
<b>video micrometers per pixel</b> : 4.2072
<b>number of segmented skeletons</b> : 26805
DOI: 10.5281/zenodo.1017927
title: Cb4852 C. Elegans Wild Isolate, N3 (Rothamsted, Uk) | 2011-03-29T12:17:27+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=aPHbDnj-GSc
<b>strain</b> : CB4852
<b>timestamp</b> : 2011-03-29T12:17:27+01:00
<b>gene</b> : -N/A-
<b>chromosome</b> : -N/A-
<b>allele</b> : -N/A-
<b>strain_description</b> : C. elegans Wild Isolate, N3 (Rothamsted, UK)
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : 135 CB4852 on food R_2011_03_29__12_17_27___7___5
<b>total time (s)</b> : 899.0
<b>frames per second</b> : 30.03
<b>video micrometers per pixel</b> : 4.2072
<b>number of segmented skeletons</b> : 26880
DOI: 10.5281/zenodo.1018207
title: Ed3054 C. Briggsae Wild Isolate (Nairobi, Kenya) | 2011-03-15T15:47:56+00:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=FnrmVbHC1P0
<b>strain</b> : ED3054
<b>timestamp</b> : 2011-03-15T15:47:56+00:00
<b>gene</b> : -N/A-
<b>chromosome</b> : -N/A-
<b>allele</b> : -N/A-
<b>strain_description</b> : C. briggsae Wild Isolate (Nairobi, Kenya)
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : 507 ED3054 on food R_2011_03_15__15_47_56___7___9
<b>total time (s)</b> : 899.0
<b>frames per second</b> : 30.03
<b>video micrometers per pixel</b> : 4.2072
<b>number of segmented skeletons</b> : 26779
DOI: 10.5281/zenodo.1023518
title: Rb1250 Acr-21(Ok1314)Iii | 2009-07-24T12:27:17+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=yH2MCvEdPyo
<b>strain</b> : RB1250
<b>timestamp</b> : 2009-07-24T12:27:17+01:00
<b>gene</b> : acr-21
<b>chromosome</b> : III
<b>allele</b> : ok1314
<b>strain_description</b> : acr-21(ok1314)III
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-21 (ok1314)III on food R_2009_07_24__12_27_17__6
<b>total time (s)</b> : 299.647
<b>frames per second</b> : 25.7069
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6196
DOI: 10.5281/zenodo.1023519
title: Rb1250 Acr-21(Ok1314)Iii | 2009-07-24T12:27:17+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=yH2MCvEdPyo
<b>strain</b> : RB1250
<b>timestamp</b> : 2009-07-24T12:27:17+01:00
<b>gene</b> : acr-21
<b>chromosome</b> : III
<b>allele</b> : ok1314
<b>strain_description</b> : acr-21(ok1314)III
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-21 (ok1314)III on food R_2009_07_24__12_27_17__6
<b>total time (s)</b> : 299.647
<b>frames per second</b> : 25.7069
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6196
DOI: 10.5281/zenodo.1031111
title: Rb1250 Acr-21(Ok1314)Iii | 2009-07-24T15:48:21+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=Gaf0bSTYxxI
<b>strain</b> : RB1250
<b>timestamp</b> : 2009-07-24T15:48:21+01:00
<b>gene</b> : acr-21
<b>chromosome</b> : III
<b>allele</b> : ok1314
<b>strain_description</b> : acr-21(ok1314)III
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-21 (ok1314)III on food L_2009_07_24__15_48_21__17
<b>total time (s)</b> : 299.499
<b>frames per second</b> : 25.8398
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6667
DOI: 10.5281/zenodo.1031112
title: Rb1250 Acr-21(Ok1314)Iii | 2009-07-24T15:48:21+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=Gaf0bSTYxxI
<b>strain</b> : RB1250
<b>timestamp</b> : 2009-07-24T15:48:21+01:00
<b>gene</b> : acr-21
<b>chromosome</b> : III
<b>allele</b> : ok1314
<b>strain_description</b> : acr-21(ok1314)III
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-21 (ok1314)III on food L_2009_07_24__15_48_21__17
<b>total time (s)</b> : 299.499
<b>frames per second</b> : 25.8398
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6667
DOI: 10.5281/zenodo.1031284
title: Rb2119 Acr-23(Ok2804)V | 2009-07-24T14:42:13+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=oRCkhpRS_TM
<b>strain</b> : RB2119
<b>timestamp</b> : 2009-07-24T14:42:13+01:00
<b>gene</b> : acr-23
<b>chromosome</b> : V
<b>allele</b> : ok2804
<b>strain_description</b> : acr-23(ok2804)V
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-23 (ok2804) on food L_2009_07_24__14_42_13__11
<b>total time (s)</b> : 299.656
<b>frames per second</b> : 26.2467
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6334
DOI: 10.5281/zenodo.1031715
title: Rb2119 Acr-23(Ok2804)V | 2009-07-24T12:37:39+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=t_MwHtMPN80
<b>strain</b> : RB2119
<b>timestamp</b> : 2009-07-24T12:37:39+01:00
<b>gene</b> : acr-23
<b>chromosome</b> : V
<b>allele</b> : ok2804
<b>strain_description</b> : acr-23(ok2804)V
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-23 (ok2804) on food L_2009_07_24__12_37_39__7
<b>total time (s)</b> : 300.002
<b>frames per second</b> : 25.8398
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6459
DOI: 10.5281/zenodo.1031716
title: Rb2119 Acr-23(Ok2804)V | 2009-07-24T12:37:39+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=t_MwHtMPN80
<b>strain</b> : RB2119
<b>timestamp</b> : 2009-07-24T12:37:39+01:00
<b>gene</b> : acr-23
<b>chromosome</b> : V
<b>allele</b> : ok2804
<b>strain_description</b> : acr-23(ok2804)V
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-23 (ok2804) on food L_2009_07_24__12_37_39__7
<b>total time (s)</b> : 300.002
<b>frames per second</b> : 25.8398
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6459
DOI: 10.5281/zenodo.1031886
title: Rb1250 Acr-21(Ok1314)Iii | 2009-07-24T14:30:50+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=XK24h0OB9Ag
<b>strain</b> : RB1250
<b>timestamp</b> : 2009-07-24T14:30:50+01:00
<b>gene</b> : acr-21
<b>chromosome</b> : III
<b>allele</b> : ok1314
<b>strain_description</b> : acr-21(ok1314)III
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-21 (ok1314)III on food R_2009_07_24__14_30_50__10
<b>total time (s)</b> : 299.23
<b>frames per second</b> : 25.4453
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6515
DOI: 10.5281/zenodo.1031887
title: Rb1250 Acr-21(Ok1314)Iii | 2009-07-24T14:30:50+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=XK24h0OB9Ag
<b>strain</b> : RB1250
<b>timestamp</b> : 2009-07-24T14:30:50+01:00
<b>gene</b> : acr-21
<b>chromosome</b> : III
<b>allele</b> : ok1314
<b>strain_description</b> : acr-21(ok1314)III
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : anticlockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-21 (ok1314)III on food R_2009_07_24__14_30_50__10
<b>total time (s)</b> : 299.23
<b>frames per second</b> : 25.4453
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6515
DOI: 10.5281/zenodo.1032392
title: Rb1226 Acr-18(Ok1285)V | 2009-07-28T12:49:48+01:00

description: This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org



<b>preview link</b> : https://www.youtube.com/watch?v=WZfQ5oGa0dw
<b>strain</b> : RB1226
<b>timestamp</b> : 2009-07-28T12:49:48+01:00
<b>gene</b> : acr-18
<b>chromosome</b> : V
<b>allele</b> : ok1285
<b>strain_description</b> : acr-18(ok1285)V
<b>sex</b> : hermaphrodite
<b>stage</b> : adult
<b>ventral_side</b> : clockwise
<b>media</b> : NGM agar low peptone
<b>arena</b> :   
  <b>style</b> : petri
  <b>size</b> : 35
  <b>orientation</b> : away
  

<b>food</b> : OP50
<b>habituation</b> : 30m wait
<b>who</b> : Laura Grundy
<b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.
<b>lab</b> :   
  <b>name</b> : William R Schafer
  <b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
  

<b>software</b> :   
  <b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)
  <b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219
  <b>featureID</b> : @OMG
  

<b>base_name</b> : acr-18 (ok1285)V on food L_2009_07_28__12_49_48__4
<b>total time (s)</b> : 299.479
<b>frames per second</b> : 25.3807
<b>video micrometers per pixel</b> : 4.57251
<b>number of segmented skeletons</b> : 6261
DOI: 10.5281/zenodo.1035898
title: Observational data of temperature and oxygen for the study "High-frequency observations of temperature and dissolved oxygen reveal under-ice convection in a large lake"

description: Matlab data for both temperature and oxygen time series between December 1, 2014, 0:00 (EST) and April 26, 2015, 0:00 (EST). Temperature profiles are sampled every 20 seconds. Dissolved oxygen are sampled every 30 minutes. The depth of each time series are written on the file name.
DOI: 10.18434/m32q1v
title: Steps to achieve quantitative measurements of microRNA using two-step droplet digital PCR

description: Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets.  It has consistently been proven to be more sensitive and direct at discerning copy numbers of DNA than other quantitative methods.  However, one of the largest obstacles to measuring microRNA (miRNA) using ddPCR is that reverse transcription efficiency depends upon the target, meaning small RNA nucleotide composition directly effects primer specificity in a manner that prevents traditional quantitation optimization strategies.  Additionally, the use of reagents that are optimized for miRNA measurements using quantitative real time PCR (qRT PCR) appear to either cause false positive or false negative detection of certain targets when used with traditional ddPCR quantification methods.  False readings are often related to using inadequate enzymes, primers and probes.  Given that two step miRNA quantification using ddPCR relies solely on reverse transcription and uses proprietary reagents previously optimized only for qRT PCR, these barriers are substantial.  Therefore, here we outline essential controls, optimization techniques, and an efficacy model to improve the quality of ddPCR miRNA measurements.  We have applied two step principles used for miRNA qRT PCR measurements and leveraged the use of synthetic miRNA targets to evaluate ddPCR following cDNA synthesis with four different commercial kits.  We have identified inefficiencies and limitations as well as proposed ways to circumvent identified obstacles.  Lastly, we show that we can apply these criteria to a model system to confidently quantify miRNA copy number.  Our measurement technique is a novel way to quantify specific miRNA copy number in a single sample, without using standard curves for individual experiments.  Our methodology can be used for validation and control measurements, as well as a diagnostic technique that allows scientists, technicians, clinicians, and regulators to base miRNA measures on a single unit of measurement rather than a ratio of values.