cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4818382.v1
title: Dataset for: Dopaminergic activation anticipates daily nursing in the rabbit

description: Maternal care is a motivated behavior and in the rabbit it is restricted to the spontaneous return of the mother to nurse her pups for just a few minutes once a day. Previously we have reported neural activation of brain areas and neuroendocrine cells after nursing. However, this daily spontaneous return suggests that the mother is in a high motivational state to nurse her pups. Here we hypothesized that during anticipation of nursing there is an activation of dopaminergic neurons of the mesolimbic system and in their target areas. Then we explored, by the expression of FOS protein, possible activation of the mesolimbic system as well as dopaminergic cells of the A10 cell group before and after nursing and in control does. Additionally, we measured FOS expression in the preoptic area and lateral septum. We found a significant increase of FOS before nursing, and a further increase after nursing, in the mesolimbic system and dopaminergic cells as well as in the preoptic area and lateral septum. Interestingly, the medial prefrontal area shows an intense activation during anticipation of nursing, which remains after nursing. We conclude that the activation of the mesolimbic system before nursing is related to the high locomotor behavior prior to the next nursing bout and support the proposal that the mother is in a high motivational state at the time of returning to the nest. The additional activation after nursing can be related to the neuroendocrine and neural consequences of the milk ejection reflex by suckling.
DOI: 10.6084/m9.figshare.4818373.v1
title: Dataset for: Light-dependent expression of a Na+/H+ exchanger 3-like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light-enhanced calcification

description: Na+/H+ exchangers (NHEs) regulate intracellular pH and ionic balance by mediating H+ efflux in exchange for Na+ uptake in a 1:1 stoichiometry. This study aimed to obtain from the ctenidium of the giant clam Tridacna squamosa (TS) the complete cDNA sequence of a NHE3-like transporter (TSNHE3), and to determine the effect of light exposure on its mRNA expression level and protein abundance therein. The coding sequence of TSNHE3 comprised 2886 bp, encoding 961 amino acids with an estimated molecular mass of 105.7 kDa. Immunofluorescence microscopy revealed that TSNHE3 was localized to the apical membrane of epithelial cells of the ctenidial filaments and the tertiary water channels. Particularly, the apical immunofluorescence of the ctenidial filaments was consistently stronger in the ctenidium of clams exposed to 12 h of light than those of the control kept in darkness. Indeed, light induced significant increases in the transcript level and protein abundance of TSNHE3/TSNHE3 in the ctenidium, indicating that the transcription and translation of TSNHE3/TSNHE3 were light-dependent. As light-enhanced calcification generates H+, the increased expression of TSNHE3/TSNHE3 in the ctenidium could be a response to augment H+ excretion in pursuance of whole-body acid-base balance during light exposure. These results signify that shell formation in giant clams requires the collaboration between the ctenidium, which is a respiratory and iono-regulatory organ, and the inner mantle, which is directly involved in the calcification process, and provide new insights into the mechanisms of light-enhanced calcification in giant clams.
DOI: 10.6084/m9.figshare.4818361
title: Dataset for: Effects of insulin and the glucagon-like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice

description: Diabetes mellitus (DM) is a worldwide disease that affects 9% of the adult world population and type 2 DM accounts for 90% of those. A common consequence of DM is kidney complications, which could lead to kidney failure. We studied the potential effects of treatment with insulin and the glucagon-like peptide 1 receptor (GLP-1R) agonist liraglutide on the diabetic kidney proteome through the use of the db/db mouse model system and mass spectrometry (MS). Multivariate analyses revealed distinct effects of insulin and liraglutide on the db/db kidney proteome, which was seen on the protein levels of, for example, pterin-4 α-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1α (PCBD1), neural precursor cell expressed developmentally down-regulated-8 (NEDD8), transcription elongation factor-B polypeptide-1 (ELOC) and hepcidin (HEPC). Furthermore, the separation of the insulin, liraglutide and vehicle db/db mouse groups in multivariate analyses was not mainly related to the albumin excretion rate (AER) or the level of glycated hemoglobin A1c (HbA1c%) in the mice. In summary, we show that insulin and liraglutide give rise to separate protein profiles in the db/db mouse kidney.
DOI: 10.6084/m9.figshare.4818364
title: Dataset for: An efficient multi-stage algorithm for full calibration of the hemodynamic model from BOLD signal responses

description: We propose a computational strategy that falls into the category of prediction/correction iterative-type approaches, for calibrating the hemodynamic model introduced by Friston et al. (2000). The proposed method is employed to estimate consecutively the values of the biophysiological system parameters and the external stimulus characteristics of the model. Numerical results corresponding to both synthetic and real functional Magnetic Resonance Imaging (fMRI) measurements for a single stimulus as well as for multiple stimuli are reported to highlight the capability of this computational methodology to fully calibrate the considered hemodynamic model.
DOI: 10.6084/m9.figshare.4818367
title: Dataset for: ACUTE ELEVATIONS IN SERUM HORMONES ARE ATTENUATED AFTER CHRONIC TRAINING WITH TRADITIONAL ISOINERTIAL BUT NOT ACCENTUATED ECCENTRIC LOADS IN STRENGTH-TRAINED MEN

description: It has been proposed that the maintenance of acute hormonal responses reveal an efficacy of a training stimulus to evoke ongoing increases in strength and muscle mass. We previously observed that maximum strength continued to improve throughout a 10-week period in an accentuated eccentric loading group (AEL) but not a traditional isoinertial loading (ISO) group. Therefore, the present study investigated whether the magnitude of acute hormonal responses was greater (i.e. maintained) in AEL compared to ISO at the end of the training period. Subjects in AEL (eccentric load = concentric load + 40%) and ISO performed experimental loading tests (3 sets of 10 repetitions in the leg press and knee extension exercises) during weeks 2 and 9 of the training period. Blood samples collected during these experimental loadings were analyzed for serum testosterone, growth hormone and cortisol concentrations. Maximum isometric knee extension torque (MVC) and lower-limb lean mass were assessed before and after 5 and 10 weeks of training. Acute testosterone, growth hormone and cortisol responses to traditional isoinertial loading were reduced at the end of the training period but were not reduced after accentuated eccentric load training (P&lt;0.05‒0.1 between-groups). Increases in MVC and lower-limb lean mass over weeks 6‒10 were greater in AEL compared to ISO (MVC: 7.3±5.4 vs. -0.4±7.2%, P=0.026 for between-group difference; lower-limb lean mass: 1.6±2.2 vs. -0.2±1.4%, P=0.063 for between-group difference). The maintenance of acute hormonal responses and continued strength gain in AEL but not ISO are consistent with the hypothesis that maintained acute responses indicate an efficacy of a training stimulus to evoke ongoing adaptation. However, since relationships between hormonal responses and training-induced adaptations were not statistically significant, the data suggest that tracking of acute hormonal responses on an individual level may not provide a sensitive enough guide for decisions regarding program design and periodization.
DOI: 10.6084/m9.figshare.4818370.v1
title: Dataset for: Blindfolding during wakefulness causes decrease in sleep slow wave activity

description: Slow wave activity (SWA, 0.5-4 Hz) represents the predominant EEG oscillatory activity during slow wave sleep (SWS). Its amplitude is considered in part a reflection of synaptic potentiation in cortical networks due to encoding of information during prior waking, with higher amplitude indicating stronger potentiation. Previous studies showed that increasing and diminishing specific motor behaviours produced corresponding changes in SWA in the respective motor cortical areas during subsequent SWS. Here, we tested whether this relationship can be generalized to the visual system, i.e., whether diminishing encoding of visual information likewise leads to a localized decrease in SWA over the visual cortex. Experiments were performed in healthy men whose eyes on two different days were or were not covered for 10.5 h before bedtime. The subject’s EEG was recorded during sleep and, after sleep, visual evoked potentials (VEPs) were recorded. SWA during non-rapid eye movement sleep (NonREM sleep) was lower after blindfolding than after eyes open (P &lt; 0.01). The decrease in SWA that was most consistent during the first 20 min of NonREM sleep, did not remain restricted to visual cortex regions, with changes over frontal and parietal cortical regions being even more pronounced. In the morning after sleep, the N75-P100 peak-to-peak-amplitude of the VEP was significantly diminished in the blindfolded condition. Our findings confirm a link between reduced wake encoding and diminished SWA during ensuing NonREM sleep, although this link appears not to be restricted to sensory cortical areas.
DOI: 10.6084/m9.figshare.4818376.v1
title: Dataset for: NF-kappaB signalling is involved in immune-modulation, but not basal functioning, of the mouse suprachiasmatic circadian clock.

description: Circadian rhythms are recurring near-twenty four hour patterns driven by an endogenous circadian timekeeping system. The master pacemaker in this system is the hypothalamuc suprachiasmatic nucleus (SCN) . Recently interest has been drawn to how the SCN clock responds to immune system stimulation. A major signalling component in the immune system is nuclear factor (NF)-kappaB. In the present study we examined the role of NF-kappaB in SCN function. Whilst serum shocked fibroblasts showed rhythmic nuclear localisation of p65 and p65-dependent transcription, there were no circadian changes in the SCN in expression of the NF-kappaB components p65, c-Rel, p-IkappaB or p-IKK. Chronic treatment with the NF-kappaB inhibitor PDTC did not impact on circadian or diurnal rhythms. Phase-shifting light pulses did not impact on SCN expression of p65, and PDTC treatment did not attenuate the behavioural or molecular response to light pulses. Peripheral treatment with lipopolysaccharide resulted in increased NF-kappaB component expression in the SCN. In vitro experiments with SCN slice cultures showed that treatment with NF-kappaB inhibitors did not markedly alter rhythmic changes in PER2::LUC expression. Further, SCN slices from nf-kappab::luc mice did not show any evidence for circadian rhythms in NF-kappaB-mediated transcription. Experiments utilising older mice (~16 months old) showed that SCN treatment in vitro with PDTC resulted in increased amplitude of rhythmic PER2::LUC expression, and LPS treatment resulted in altered PER2::LUC rhythm acrophase. Overall, we interpret our results as providing evidence for the involvement of NF-kappaB in the suprachiasmatic circadian clock following immune stimulation, but not under basal conditions.
DOI: 10.6084/m9.figshare.4818355
title: Dataset for: Edaravone attenuates intracerebroventricular streptozotocin-induced cognitive impairment in rats

description: Alzheimer’s disease is a major cause of dementia worldwide. Edaravone, a potent free radical scavenger, is reported to be neuroprotective. The present study was designed to investigate the effect of chronic edaravone administration on intracerebroventricular-streptozotocin (ICV-STZ) induced cognitive impairment in male Wistar rats. Cognitive impairment was developed by single ICV-STZ (3 mg/kg) injection bilaterally on day 1. Edaravone (1, 3 and 10 mg/kg, orally, once daily) was administered for 28 days. Morris water maze and passive avoidance tests were used to assess cognitive functions at baseline and on days 14 and 28. ICV-STZ caused cognitive impairment as evidenced by increased escape latency and decreased time spent in target quadrant in the Morris water maze test and reduced retention latency in the passive avoidance test. STZ caused increase in oxidative stress, cholinesterases, inflammatory cytokines and protein expression of ROCK-II and decrease in protein expression of ChAT. Edaravone ameliorated the STZ-induced cognitive impairment. STZ-induced increase in oxidative stress and increased levels of pro-inflammatory cytokines (TNF-α, IL-1β) were mitigated by edaravone. Edaravone also prevented STZ-induced increased protein expression of ROCK-II. Moreover, edaravone significantly prevented STZ-induced increased activity of cholinesterases in the cortex and hippocampus. The decreased expression of ChAT caused by STZ was brought towards normal by edaravone in the hippocampus. The results thus show that edaravone is protective against STZ-induced cognitive impairment, oxidative stress, cholinergic dysfunction and altered protein expressions. This study thus suggests the potential of edaravone as an adjuvant in the treatment of Alzheimer’s disease.
DOI: 10.6084/m9.figshare.4818361.v1
title: Dataset for: Effects of insulin and the glucagon-like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice

description: Diabetes mellitus (DM) is a worldwide disease that affects 9% of the adult world population and type 2 DM accounts for 90% of those. A common consequence of DM is kidney complications, which could lead to kidney failure. We studied the potential effects of treatment with insulin and the glucagon-like peptide 1 receptor (GLP-1R) agonist liraglutide on the diabetic kidney proteome through the use of the db/db mouse model system and mass spectrometry (MS). Multivariate analyses revealed distinct effects of insulin and liraglutide on the db/db kidney proteome, which was seen on the protein levels of, for example, pterin-4 α-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1α (PCBD1), neural precursor cell expressed developmentally down-regulated-8 (NEDD8), transcription elongation factor-B polypeptide-1 (ELOC) and hepcidin (HEPC). Furthermore, the separation of the insulin, liraglutide and vehicle db/db mouse groups in multivariate analyses was not mainly related to the albumin excretion rate (AER) or the level of glycated hemoglobin A1c (HbA1c%) in the mice. In summary, we show that insulin and liraglutide give rise to separate protein profiles in the db/db mouse kidney.
DOI: 10.6084/m9.figshare.4818364.v1
title: Dataset for: An efficient multi-stage algorithm for full calibration of the hemodynamic model from BOLD signal responses

description: We propose a computational strategy that falls into the category of prediction/correction iterative-type approaches, for calibrating the hemodynamic model introduced by Friston et al. (2000). The proposed method is employed to estimate consecutively the values of the biophysiological system parameters and the external stimulus characteristics of the model. Numerical results corresponding to both synthetic and real functional Magnetic Resonance Imaging (fMRI) measurements for a single stimulus as well as for multiple stimuli are reported to highlight the capability of this computational methodology to fully calibrate the considered hemodynamic model.
DOI: 10.6084/m9.figshare.4818367.v1
title: Dataset for: ACUTE ELEVATIONS IN SERUM HORMONES ARE ATTENUATED AFTER CHRONIC TRAINING WITH TRADITIONAL ISOINERTIAL BUT NOT ACCENTUATED ECCENTRIC LOADS IN STRENGTH-TRAINED MEN

description: It has been proposed that the maintenance of acute hormonal responses reveal an efficacy of a training stimulus to evoke ongoing increases in strength and muscle mass. We previously observed that maximum strength continued to improve throughout a 10-week period in an accentuated eccentric loading group (AEL) but not a traditional isoinertial loading (ISO) group. Therefore, the present study investigated whether the magnitude of acute hormonal responses was greater (i.e. maintained) in AEL compared to ISO at the end of the training period. Subjects in AEL (eccentric load = concentric load + 40%) and ISO performed experimental loading tests (3 sets of 10 repetitions in the leg press and knee extension exercises) during weeks 2 and 9 of the training period. Blood samples collected during these experimental loadings were analyzed for serum testosterone, growth hormone and cortisol concentrations. Maximum isometric knee extension torque (MVC) and lower-limb lean mass were assessed before and after 5 and 10 weeks of training. Acute testosterone, growth hormone and cortisol responses to traditional isoinertial loading were reduced at the end of the training period but were not reduced after accentuated eccentric load training (P&lt;0.05‒0.1 between-groups). Increases in MVC and lower-limb lean mass over weeks 6‒10 were greater in AEL compared to ISO (MVC: 7.3±5.4 vs. -0.4±7.2%, P=0.026 for between-group difference; lower-limb lean mass: 1.6±2.2 vs. -0.2±1.4%, P=0.063 for between-group difference). The maintenance of acute hormonal responses and continued strength gain in AEL but not ISO are consistent with the hypothesis that maintained acute responses indicate an efficacy of a training stimulus to evoke ongoing adaptation. However, since relationships between hormonal responses and training-induced adaptations were not statistically significant, the data suggest that tracking of acute hormonal responses on an individual level may not provide a sensitive enough guide for decisions regarding program design and periodization.
DOI: 10.6084/m9.figshare.4818352
title: Dataset for: Estimating marginal causal effects in a secondary analysis of case-control data

description: When an initial case-control study is performed data can be used in a secondary analysis to evaluate the effect of the case-defining event on later outcomes. In this paper, we study the example in which the role of the event is changed from a response variable to a treatment of interest. If the aim is to estimate marginal effects, such as average effects in the population, the sampling scheme needs to be adjusted for. We study estimators of the average effect of the treatment in a secondary analysis of matched and unmatched case-control data where the probability of being a case is known. For a general class of estimators, we show the components of the bias resulting from ignoring the sampling scheme and demonstrate a design-weighted matching estimator of the average causal effect. In simulations, the finite sample properties of the design-weighted matching estimator are studied. Using a Swedish diabetes incidence register with a matched case-control design we study the effect of childhood onset diabetes on the use of anti-depressant medication as an adult.
DOI: 10.6084/m9.figshare.4818346
title: Dataset for: Validation of a routine two-sample iohexol plasma clearance assessment of GFR and an evaluation of common endogenous markers in a rat model of CKD

description: Endogenous markers of kidney function are insensitive to early declines in GFR and in rodent models, validated, practical alternatives are unavailable. In this study we determined GFR by modelling the plasma clearance of two compounds, iohexol and inulin, and compared the findings to common endogenous markers. All plasma clearance methods of both iohexol and inulin detected a decline in renal function weeks prior to any increase in endogenous marker. Iohexol plasma clearance and inulin plasma clearance had very high agreement and minimal bias when using 12-sample models. However, only iohexol could be accurately simplified to a two-sample, 1-compartment estimation strategy. Following an IV injection of low-dose iohexol and two timed blood samples at 30 and 90 minutes, one can accurately approximate a complex 12-sample strategy of plasma clearance. This method is simple enough to use in routine, longitudinal analysis of larger cohort animal studies.
DOI: 10.6084/m9.figshare.4818349
title: Dataset for: Statistical Consequences of a Successful Lung Allocation System - Recovering Information and Reducing Bias in Models for Urgency

description: The lung allocation system has reduced the number of waitlist deaths by ranking transplant candidates based on a lung allocation score (LAS) that requires estimation of the current 1-year restricted mean waitlist survival (urgency). Fewer waitlist deaths and the systematic removal of candidates from the waitlist for transplantation present statistical challenges that must be addressed when using recent waitlist data. Multiple overlapping 1-year follow-up windows are used in a restricted mean model that estimates patient urgency based on updated risk factors at the start of the window. In simulation studies, our proposed multiple imputation procedure was able to produce unbiased parameter estimates with similar efficiency to those obtained if censoring had never occurred. The analysis of 10,740 lung transplant candidates revealed that for most risk factors incorporating additional follow-up windows produced more efficient estimates.
DOI: 10.6084/m9.figshare.4818355.v1
title: Dataset for: Edaravone attenuates intracerebroventricular streptozotocin-induced cognitive impairment in rats

description: Alzheimer’s disease is a major cause of dementia worldwide. Edaravone, a potent free radical scavenger, is reported to be neuroprotective. The present study was designed to investigate the effect of chronic edaravone administration on intracerebroventricular-streptozotocin (ICV-STZ) induced cognitive impairment in male Wistar rats. Cognitive impairment was developed by single ICV-STZ (3 mg/kg) injection bilaterally on day 1. Edaravone (1, 3 and 10 mg/kg, orally, once daily) was administered for 28 days. Morris water maze and passive avoidance tests were used to assess cognitive functions at baseline and on days 14 and 28. ICV-STZ caused cognitive impairment as evidenced by increased escape latency and decreased time spent in target quadrant in the Morris water maze test and reduced retention latency in the passive avoidance test. STZ caused increase in oxidative stress, cholinesterases, inflammatory cytokines and protein expression of ROCK-II and decrease in protein expression of ChAT. Edaravone ameliorated the STZ-induced cognitive impairment. STZ-induced increase in oxidative stress and increased levels of pro-inflammatory cytokines (TNF-α, IL-1β) were mitigated by edaravone. Edaravone also prevented STZ-induced increased protein expression of ROCK-II. Moreover, edaravone significantly prevented STZ-induced increased activity of cholinesterases in the cortex and hippocampus. The decreased expression of ChAT caused by STZ was brought towards normal by edaravone in the hippocampus. The results thus show that edaravone is protective against STZ-induced cognitive impairment, oxidative stress, cholinergic dysfunction and altered protein expressions. This study thus suggests the potential of edaravone as an adjuvant in the treatment of Alzheimer’s disease.