DOI: 10.22002/e7ncb-wng76
title: Caulobacter crescentus

description: wild type CB15N. not included in paper?  second axis tilt range is  +/- 62 degrees. extended stationary phase, ATCC PYE formula, stressed cells, 2nd bacterium found
description: <strong>Tilt Series Date:</strong> 2004-06-04</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Dual Axis, tilt range: (-60.0°, 62.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep & data collection: D. Prabha Dias; processing: Bingni Brunton (prev. Wen)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> extended stationary phase, tapwater ATCC (PYE) media, wild-type CB15N</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, 10nm gold on grids & in suspension, 100% humidity, -3 offset, 1 sec blot, 2 sec drain, gold on </p>
  pdd2004-06-04-1_slicer4652.jpg: Granule pdd2004-06-04-1_slicer4653.jpg: Unknown pdd2004-06-04-1_slicer4654.jpg: Peptidoglycan pdd2004-06-04-1_slicer4655.jpg: Unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/rawdata/</p>CC04Jun04e100t01a.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/rawdata/CC04Jun04e100t01a.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC04Jun04e100t01b.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/rawdata/CC04Jun04e100t01b.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cbin.rec,  Reconstruction (Pixel Size 2.4 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/3dimage_184/Cbin.rec"
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        Cbina.rec,  Reconstruction (Pixel Size 2.4 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/3dimage_185/Cbina.rec"
        > <i class="download icon"></i> Download </a></p>

        Cbinb.rec,  Reconstruction (Pixel Size 2.4 nm) Notes for Full: Keywords:  .  stationary phase .  100 electrons dose per tilt series .  CB15 .  GIFCCD, 985.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/3dimage_186/Cbinb.rec"
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        CC04Jun_a.mov,  file associated to 3D Image #186, 23.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-1/file_265/CC04Jun_a.mov"
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        CC04Jun_b.mov,  file associated to 3D Image #186, 22.8 MB  
        
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