cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/aznvj-12236
title: Helicobacter pylori 26695

description: Cells are sick. Don't have flagella. Only see partial motors.
description: <strong>Tilt Series Date:</strong> 2013-10-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in Brucella broth (Fluka) + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-25/rawdata/</p>20131009_Hpylori_26695_BB_10025.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-25/rawdata/20131009_Hpylori_26695_BB_10025.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131009_Hpylori_26695_BB_10025_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-25/3dimage_32180/20131009_Hpylori_26695_BB_10025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/en2g3-22v98
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug).
description: <strong>Tilt Series Date:</strong> 2012-11-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-10/rawdata/</p>12nov29_TRIM5_21R_delSpry_cart1_AT2BT1man_002.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-10/rawdata/12nov29_TRIM5_21R_delSpry_cart1_AT2BT1man_002.mrc"
        > <i class="download icon"></i> Download </a></p>

        12nov29_TRIM5_21R_delSpry_cart1_AT2BT1man_002_part122_5.rec,  Reconstruction (Pixel Size 1.96 nm), 530.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-10/3dimage_24059/12nov29_TRIM5_21R_delSpry_cart1_AT2BT1man_002_part122_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.h24qcp
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Enteromius amboseli, Enteromius apleurogramma, Enteromius anema, Enteromius annectens, Enteromius anniae, Enteromius anoplus, Enteromius ansorgii, Enteromius arambourgi, Enteromius arcislongae, Enteromius argenteus, Enteromius aspilus, Enteromius atakorensis, Enteromius atkinsoni, Enteromius atromaculatus, Enteromius bagbwensis, Enteromius barnardi, Enteromius barotseensis, Enteromius baudoni, Enteromius bawkuensis, Enteromius bifrenatus, Enteromius bigornei, Enteromius boboi, Enteromius bourdariei, Enteromius brachygramma, Enteromius brazzai, Enteromius breviceps, Enteromius brevidorsalis, Enteromius brevilateralis, Enteromius brevipinnis, Enteromius brichardi, Enteromius cadenati, Enteromius callipterus, Enteromius camptacanthus, Enteromius candens, Enteromius carcharhinoides, Enteromius carens, Enteromius castrasibutum, Enteromius catenarius, Enteromius caudosignatus, Enteromius cercops, Enteromius chicapaensis, Enteromius chiumbeensis, Enteromius chlorotaenia, Enteromius choloensis, Enteromius citrinus, Enteromius clauseni, Enteromius collarti, Enteromius condei, Enteromius crocodilensis, Enteromius deguidei, Enteromius deserti, Enteromius dialonensis, Enteromius diamouanganai, Enteromius ditinensis, Enteromius dorsolineatus, Enteromius eburneensis, Enteromius erythrozonus, Enteromius eutaenia, Enteromius evansi, Enteromius fasciolatus, Enteromius foutensis, Enteromius greenwoodi, Enteromius guildi, Enteromius guineensis, Enteromius guirali, Enteromius gurneyi, Enteromius haasianus, Enteromius holotaenia, Enteromius hospes, Pseudobarbus hospes, Enteromius huguenyi, Enteromius hulstaerti, Enteromius humeralis, Enteromius inaequalis, Enteromius innocens, Enteromius jacksoni, Enteromius jae, Enteromius janssensi, Enteromius kamolondoensis, Enteromius kerstenii, Enteromius kessleri, Enteromius kissiensis, Enteromius kuiluensis, Enteromius lamani, Enteromius laticeps, Enteromius lauzannei, Enteromius leonensis, Enteromius liberiensis, Enteromius lineomaculatus, Enteromius litamba, Enteromius lornae, Enteromius loveridgii, Enteromius lufukiensis, Enteromius luikae, Enteromius lujae, Enteromius lukindae, Enteromius lukusiensis, Enteromius luluae, Enteromius machadoi, Enteromius macinensis)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((59.2345 -15.8834,59.2192 -15.9401,59.9717 -15.9401,59.949 -15.8852,59.5935 -15.7371,59.2345 -15.8834))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008471-250827131500795/datasets/export for details.
DOI: 10.22002/wvzkf-0xg51
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-71/rawdata/</p>20131025_Janelia_YWC043_20017.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-71/rawdata/20131025_Janelia_YWC043_20017.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_20017_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-71/3dimage_33545/20131025_Janelia_YWC043_20017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wvtwh-pgw40
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC117
description: <strong>Tilt Series Date:</strong> 2014-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 180 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-28-28/rawdata/</p>20140928_YWC117_20012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-28-28/rawdata/20140928_YWC117_20012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140928_YWC117_20012_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-28-28/3dimage_46811/20140928_YWC117_20012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t8zw9-13n13
title: Myxococcus xanthus delta-pilA

description: grid box YWC059
description: <strong>Tilt Series Date:</strong> 2013-12-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-18/rawdata/</p>20131228_Janelia_YWC059_10007.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-18/rawdata/20131228_Janelia_YWC059_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131228_Janelia_YWC059_10007_part131_6.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-28-18/3dimage_37196/20131228_Janelia_YWC059_10007_part131_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9jv0g-6yq83
title: Hippocampal Neurons (cultured)

description: Plunge frozen at DIV 14
description: <strong>Tilt Series Date:</strong> 2014-10-18</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-10-18-14/rawdata/</p>101814_hipp_neuron_27kx0015.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-10-18-14/rawdata/101814_hipp_neuron_27kx0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        101814_hipp_neuron_27kx0015_part121_2.rec,  Reconstruction (Pixel Size 1.64 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-10-18-14/3dimage_47224/101814_hipp_neuron_27kx0015_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wx6ez-a0h72
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-35/rawdata/</p>20141022_YWC121_40003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-35/rawdata/20141022_YWC121_40003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_40003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-35/3dimage_47434/20141022_YWC121_40003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.kbxn84
title: Occurrence Download

description: A dataset containing 20 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Enteromius amboseli, Enteromius apleurogramma, Enteromius anema, Enteromius annectens, Enteromius anniae, Enteromius anoplus, Enteromius ansorgii, Enteromius arambourgi, Enteromius arcislongae, Enteromius argenteus, Enteromius aspilus, Enteromius atakorensis, Enteromius atkinsoni, Enteromius atromaculatus, Enteromius bagbwensis, Enteromius barnardi, Enteromius barotseensis, Enteromius baudoni, Enteromius bawkuensis, Enteromius bifrenatus, Enteromius bigornei, Enteromius boboi, Enteromius bourdariei, Enteromius brachygramma, Enteromius brazzai, Enteromius breviceps, Enteromius brevidorsalis, Enteromius brevilateralis, Enteromius brevipinnis, Enteromius brichardi, Enteromius cadenati, Enteromius callipterus, Enteromius camptacanthus, Enteromius candens, Enteromius carcharhinoides, Enteromius carens, Enteromius castrasibutum, Enteromius catenarius, Enteromius caudosignatus, Enteromius cercops, Enteromius chicapaensis, Enteromius chiumbeensis, Enteromius chlorotaenia, Enteromius choloensis, Enteromius citrinus, Enteromius clauseni, Enteromius collarti, Enteromius condei, Enteromius crocodilensis, Enteromius deguidei, Enteromius deserti, Enteromius dialonensis, Enteromius diamouanganai, Enteromius ditinensis, Enteromius dorsolineatus, Enteromius eburneensis, Enteromius erythrozonus, Enteromius eutaenia, Enteromius evansi, Enteromius fasciolatus, Enteromius foutensis, Enteromius greenwoodi, Enteromius guildi, Enteromius guineensis, Enteromius guirali, Enteromius gurneyi, Enteromius haasianus, Enteromius holotaenia, Enteromius hospes, Pseudobarbus hospes, Enteromius huguenyi, Enteromius hulstaerti, Enteromius humeralis, Enteromius inaequalis, Enteromius innocens, Enteromius jacksoni, Enteromius jae, Enteromius janssensi, Enteromius kamolondoensis, Enteromius kerstenii, Enteromius kessleri, Enteromius kissiensis, Enteromius kuiluensis, Enteromius lamani, Enteromius laticeps, Enteromius lauzannei, Enteromius leonensis, Enteromius liberiensis, Enteromius lineomaculatus, Enteromius litamba, Enteromius lornae, Enteromius loveridgii, Enteromius lufukiensis, Enteromius luikae, Enteromius lujae, Enteromius lukindae, Enteromius lukusiensis, Enteromius luluae, Enteromius machadoi, Enteromius macinensis)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((40.4338 -16.6515,40.833 -15.9401,37.1382 -15.9401,37.1382 -18.642,37.5549 -18.1303,38.5882 -17.6405,39.1071 -17.6835,39.6106 -17.1715,40.0468 -17.0272,40.4338 -16.6515))"
 ]
}

 The dataset includes 20 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008472-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/8gnmb-t3e28
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 10 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-01-14</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-29/rawdata/</p>stiv10h40019.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-29/rawdata/stiv10h40019.mrc"
        > <i class="download icon"></i> Download </a></p>

        stiv10h40019_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-14-29/3dimage_37723/stiv10h40019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/61fs2-hn510
title: Myxococcus xanthus delta-pilP

description: grid box YWC064
description: <strong>Tilt Series Date:</strong> 2013-12-30</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-30-7/rawdata/</p>20131230_YWC064_10007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
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        20131230_YWC064_10007_part131_8.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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