DOI: 10.5061/dryad.m3685
title: Data from: Red fluorescence in coral larvae is associated with a diapause-like state

description: Effective dispersal across environmental gradients is the key to species
 resilience to environmental perturbation, including climate change. Coral
 reefs are among the most sensitive ecosystems to global warming, but
 factors predicting coral dispersal potential remain unknown. In a
 reef-building coral Acropora millepora, larval fluorescence emerged as a
 possible indicator of dispersal potential since it correlates with
 responsiveness to a settlement cue. Here we show that gene expression in
 red fluorescent larvae of A. millepora is correlated with diapause-like
 characteristics highly likely to be associated with extended dispersal. We
 compared gene expression among three larval fluorescent morphs under three
 colored light treatments. While color morphs did not differ in their gene
 expression responses to light color, red larvae demonstrated gene
 expression signatures of cell cycle arrest and decreased transcription
 accompanied by elevated ribosome production and heightened defenses
 against oxidative stress. A meta-analysis revealed that this profile was
 highly similar to the signatures of elevated thermal tolerance in the same
 coral species, and moreover, functionally resembled diapause states in an
 insect and a nematode. Our results support a connection between red
 fluorescence and long-range dispersal, which offers a new perspective on
 the molecular underpinnings of coral larval dispersal and the biological
 function of GFP-like fluorescent proteins.
description: DESeq2_ColorMorphGER script for all DESeq2 analysis. allcounts_Jan102015.txt is loaded into this script.allcounts_Jan102015Table of count data from processed reads. Columns are samples and rows are genes.VSDandPVALS_AllVariance stabilized gene expression data for each sample (columns 1-18) and pvalues/FDR corrected pvalues (columns 19-33) associated with each statistical test. Rows are genes. Extensions .c, .t, and .i are results of LRT tests for fluroescent phenotype, light treatment and the interation, respectively. Extensions .Only are Wald test results comparing one color larvae to the other two. Extensions .V are Wald test results comparing only two of the morphs.ParentageR script for characterizing Parentage of individual color morphs based on microsatellites. ParentsRJune2015.csv and props_stacked.csv are loaded into this script.ParentsRJune2015Results of parentage analysis based on microsatellites for individual color morphsprops_stackedProportions of each color morph produced by each parent, used for plotting figure 1D.KOGtableDeltaRanksThis table includes the delta rank values for each KOG category (rows) in each dataset (columns). Explanations of each dataset (column) is the same as in the Fig. 2 legend.KOGtablePvalsTable of FDR corrected pvalues for each KOG category (rows) in each dataset (columns).GO_KOGinputfilesInput files for KOG meta-analysis. Each file has two columns, gene identifier and uncorrected log-transformed p-values (negative if the gene was down-regulated, positive if up-regulated). The file names refers to the datasets specified in the columns of Figure 2A. Two datasets were taken from (Dixon et al 2015), 'tolerance' and 'adult.lt'. Two datasets were taken from (Meyer et al 2011), 'larvae.st' and 'larvae.lt'. One dataset was taken from (Gong et al 2013), diapause, and one dataset was taken from (Sinha et al 2012), dauer. Descriptions of each dataset are given in the Fig 2 legend.