cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.bx2hhs
title: Occurrence Download

description: A dataset containing 8512 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ribes cynosbati"
 ]
}

 The dataset includes 8512 records from 195 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013704-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/qxe18-cxh10
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-26/rawdata/</p>28.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-26/rawdata/28.mrc"
        > <i class="download icon"></i> Download </a></p>

        28_part122_2.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-26/3dimage_24405/28_part122_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dy3h6-65w34
title: Bdellovibrio/E. coli minicell

description: Sample box: YWC053Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion: mixing time 36 min
description: <strong>Tilt Series Date:</strong> 2013-12-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 36 min. Pellet of 10 nm gold beads were first resuspent thoroughly and 20 ul cells were then gently mixed in by tapping the tube</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-12-06-28_slicer6433.jpg: chemoreceptor ycw2013-12-06-28_slicer6434.jpg: nucleoid ycw2013-12-06-28_slicer6437.jpg: chemoreceptor .  filament ycw2013-12-06-28_slicer6438.jpg: filament ycw2013-12-06-28_slicer6439.jpg: filament ycw2013-12-06-28_slicer6440.jpg: filament ycw2013-12-06-28_slicer6441.jpg: pilli ycw2013-12-06-28_slicer7520.jpg: Bdellovibrio bacteriovorus   HD100 .  pilli .  appendage .  secretion system? .  5.5 nm thick
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-28/rawdata/</p>montage_25x250020.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-28/rawdata/montage_25x250020.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_25x250020_part131_2.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-28/3dimage_35766/montage_25x250020_part131_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5g2md-x7x75
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-13/rawdata/</p>13.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-13/rawdata/13.mrc"
        > <i class="download icon"></i> Download </a></p>

        13_part122_5.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-13/3dimage_24390/13_part122_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.caq9q7
title: Occurrence Download

description: A dataset containing 769 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aedes albopictus",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "Year is 2024",
 "Geometry POLYGON((-25 70, 40 70, 40 35, -25 35, -25 70))"
 ]
}

 The dataset includes 769 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013705-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/863ns-zxr58
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
  lga2010-01-04-52_slicer9620.jpg: Sulfolobus solfataricus .  nested vesicles
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-52/rawdata/</p>51.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-52/rawdata/51.mrc"
        > <i class="download icon"></i> Download </a></p>

        51_part122_22.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-52/3dimage_24427/51_part122_22.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fchxt-5v285
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-25/rawdata/</p>27.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-25/rawdata/27.mrc"
        > <i class="download icon"></i> Download </a></p>

        27_part122_10.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-25/3dimage_24399/27_part122_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dx4gh-76577
title: Bdellovibrio/E. coli minicell

description: Sample box: YWC053Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion: mixing time 36 min
description: <strong>Tilt Series Date:</strong> 2013-12-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 36 min. Pellet of 10 nm gold beads were first resuspent thoroughly and 20 ul cells were then gently mixed in by tapping the tube</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-12-06-57_slicer8007.jpg: Bdellovibrio bacteriovorus .  nucleoid ycw2013-12-06-57_slicer8008.jpg: Bdellovibrio bacteriovorus .  pili (7 nm) ycw2013-12-06-57_slicer8012.jpg: Bdellovibrio bacteriovorus .  peptidoglycan ycw2013-12-06-57_slicer8013.jpg: Bdellovibrio bacteriovorus .  unknown ycw2013-12-06-57_slicer8014.jpg: Bdellovibrio bacteriovorus .  chemoreceptor ycw2013-12-06-57_slicer8015.jpg: Bdellovibrio bacteriovorus .  flagella ycw2013-12-06-57_slicer8017.jpg: Bdellovibrio bacteriovorus .  nucleoid .  REZ ribosome exclusion zone
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-57/rawdata/</p>montage_25x250001.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-57/rawdata/montage_25x250001.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_25x250001_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-57/3dimage_35791/montage_25x250001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/37et3-5m820
title: H.pylori 26695 delta-cagA / AGS

description: grid box: YWC082
description: <strong>Tilt Series Date:</strong> 2014-02-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori & AGS cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-02-15-9_slicer3707.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Many tubes extending from Hp cell (top) to what might be a human cell on bottom.  Cells look very unhealthy. T4SS ycw2014-02-15-9_slicer3708.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Many tubes extending from Hp cell(s).  Possibly T4SS related. ycw2014-02-15-9_slicer18639.jpg: H. pylori membrane vesicles & clathrin coated vesicles in co-culture with AGS ycw2014-02-15-9_slicer18640.jpg: H. pylori membrane tubes. Periodicity.
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-15-9/rawdata/</p>20140215_YWC082_20002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-15-9/rawdata/20140215_YWC082_20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140215_YWC082_20002_part121_1.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-15-9/3dimage_38980/20140215_YWC082_20002_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ch670-rfx96
title: H.pylori 26695 delta-cagH / AGS

description: grid box: YWC080
description: <strong>Tilt Series Date:</strong> 2014-02-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori & AGS cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-02-14-15_slicer3690.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Tube projecting from top of Hp cell.  Possibly T4SS related. ycw2014-02-14-15_slicer3691.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Small tubes (arrows) extending from big tube. Possibly T4SS related. ycw2014-02-14-15_slicer3692.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Tube extending from Hp cell.  Part of the tube is in cross section.  Possible T4SS related.
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-15/rawdata/</p>20140214_YWC080_10012.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-15/rawdata/20140214_YWC080_10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140214_YWC080_10012_part121_20.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-15/3dimage_38944/20140214_YWC080_10012_part121_20.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.eg8zz2
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Phalaris angusta",
 "Country is France",
 "HasCoordinate is true"
 ]
}

 The dataset includes 15 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013707-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.