cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25402/bio.24745272
title: Free hemoglobin determination atpatients’ bedside to evaluate hemolysis: supplementary data

description: Background: The authors report the relevance of using a point of care test (Helge R ? ) for free hemoglobindetermination and concordance of the values the with Cobas R ? 8000 and spectrophotometer methods.Results: The within-run of the point of care test was &lt;3%. Good correlations among the threemethods were observed and an acceptable concordance for hemolysis index values from 50 mg/dl.An excellent agreement between the Cobas 8000 and the spectrophotometer was found. Conclusion:Automated methods represent methods of choice for free hemoglobin determination. An advantageof the Helge system is that it can be applied to samples experiencing a delay in evaluation due to thelong distance between the collection site and the central laboratory. Another advantage is its use at thebedside, in the monitoring of extracorporeal membrane oxygenation patients.
DOI: 10.25402/bio.24745272.v1
title: Free hemoglobin determination atpatients’ bedside to evaluate hemolysis: supplementary data

description: Background: The authors report the relevance of using a point of care test (Helge R ? ) for free hemoglobindetermination and concordance of the values the with Cobas R ? 8000 and spectrophotometer methods.Results: The within-run of the point of care test was &lt;3%. Good correlations among the threemethods were observed and an acceptable concordance for hemolysis index values from 50 mg/dl.An excellent agreement between the Cobas 8000 and the spectrophotometer was found. Conclusion:Automated methods represent methods of choice for free hemoglobin determination. An advantageof the Helge system is that it can be applied to samples experiencing a delay in evaluation due to thelong distance between the collection site and the central laboratory. Another advantage is its use at thebedside, in the monitoring of extracorporeal membrane oxygenation patients.
DOI: 10.25549/examiner-c44-15314
title: Navy flyers back (Burbank); men of Patrol Squadron VP 772, 1951

description: 12 images. Navy flyers back (Burbank); men of Patrol Squadron VP 772, 9 August 1951. Lieutenant Warren Harrison; Stephen Harrison -- 2 years; Joe Pummill; Mrs. Mildred Pummill (wife); James F. Bowman; Lieutenant M.G. Smith.
DOI: 10.25402/bio.24799887
title: Bioanalysis of the Bicycle R ? toxin conjugateBT5528 and released monomethyl auristatinE via liquid chromatography–tandem massspectrometry: supplementary materials

description: Background: The Bicycle R ? toxin conjugate BT5528 is a novel peptide therapeutic conjugated to thecytotoxic agent monomethyl auristatin E (MMAE). A bioanalytical assay was developed to quantifyBT5528 and unconjugated MMAE in human plasma. Methodology: BT5528 quantitation used a proteinprecipitation procedure followed by LC–MS/MS detection. Quantitation of MMAE required a selectiveoffline and online solid-phase extraction with detection via LC–MS/MS. Results: BT5528 was quantifiedover the assay range of 5–2500 ng/ml and free MMAE was quantified over the assay range of 0.05–50 ng/ml. Conclusion: Bioanalytical methods were used in the bioanalysis of intact BT5528 and releasedMMAE, in a phase I/IIa clinical trial; to date, over 2000 human patient samples have been analyzed.
DOI: 10.25402/bio.24799887.v1
title: Bioanalysis of the Bicycle R ? toxin conjugateBT5528 and released monomethyl auristatinE via liquid chromatography–tandem massspectrometry: supplementary materials

description: Background: The Bicycle R ? toxin conjugate BT5528 is a novel peptide therapeutic conjugated to thecytotoxic agent monomethyl auristatin E (MMAE). A bioanalytical assay was developed to quantifyBT5528 and unconjugated MMAE in human plasma. Methodology: BT5528 quantitation used a proteinprecipitation procedure followed by LC–MS/MS detection. Quantitation of MMAE required a selectiveoffline and online solid-phase extraction with detection via LC–MS/MS. Results: BT5528 was quantifiedover the assay range of 5–2500 ng/ml and free MMAE was quantified over the assay range of 0.05–50 ng/ml. Conclusion: Bioanalytical methods were used in the bioanalysis of intact BT5528 and releasedMMAE, in a phase I/IIa clinical trial; to date, over 2000 human patient samples have been analyzed.
DOI: 10.25549/examiner-c44-15304
title: Mrs. F.S. McGinnis; society, 1951

description: 5 images. Mrs. F.S. McGinnis; society, 22 April 1951. Mr. & Mrs. Frank H. Powell, Jr.; Mrs. Felix S. McGinnis; Mr. & Mrs. William C. Brophy.
DOI: 10.25402/bio.24972819
title: LC–MS/MS method for simultaneousestimation of raloxifene, cladrin in ratplasma: application in pharmacokineticstudies – supplementary materials

description: Aim: A newer LC–MS/MS method was developed and validated for the simultaneous quantification ofraloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 μ)Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v)as mobile phase by using biological matrices in female Sprague–Dawley rats using–MS/MS. Results: Thedeveloped method was found to be linear over the concentration ranges of 1–600 ng/ml, and lowerlimit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showedCmax = 4.23 ± 0.61, 26.97 ± 1.14 ng/ml, at Tmax(h) 5.5 ± 1.00 and 3.5 ± 1.00, respectively. Conclusion:Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL forosteoporosis treatment.
DOI: 10.25402/bio.24972819.v1
title: LC–MS/MS method for simultaneousestimation of raloxifene, cladrin in ratplasma: application in pharmacokineticstudies – supplementary materials

description: Aim: A newer LC–MS/MS method was developed and validated for the simultaneous quantification ofraloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 μ)Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v)as mobile phase by using biological matrices in female Sprague–Dawley rats using–MS/MS. Results: Thedeveloped method was found to be linear over the concentration ranges of 1–600 ng/ml, and lowerlimit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showedCmax = 4.23 ± 0.61, 26.97 ± 1.14 ng/ml, at Tmax(h) 5.5 ± 1.00 and 3.5 ± 1.00, respectively. Conclusion:Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL forosteoporosis treatment.
DOI: 10.25402/bio.25003553
title: Validation of Mitra ?VAMS as a bloodcollection technique for trace elementsanalysis using ICP-MS/MS: supplementary data

description: Background: Clinical dosage of toxic and essential elements in blood is well established and the collectionmethod is still by venipuncture. This method has drawbacks and is not suited for everyone. Volumetricabsorptive microsampling (VAMS) has been shown to have advantages over venipuncture. Materials& methods: Using inductively coupled plasma tandem mass spectrometry, a method for quantifyingelements in whole blood sampled on VAMS was developed/validated.Method’s performance was assessedby comparison with whole blood results. Results: Validation and performance assessment tests tend toshow that most of the targeted elements provides accurate and reproducible results comparing to amethod of reference. Conclusion: Overall, VAMS presents good preliminary results to eventually becomean alternative to venipuncture for blood sampling for some trace elements analysis purposes.
DOI: 10.25402/bio.25003553.v1
title: Validation of Mitra ?VAMS as a bloodcollection technique for trace elementsanalysis using ICP-MS/MS: supplementary data

description: Background: Clinical dosage of toxic and essential elements in blood is well established and the collectionmethod is still by venipuncture. This method has drawbacks and is not suited for everyone. Volumetricabsorptive microsampling (VAMS) has been shown to have advantages over venipuncture. Materials& methods: Using inductively coupled plasma tandem mass spectrometry, a method for quantifyingelements in whole blood sampled on VAMS was developed/validated.Method’s performance was assessedby comparison with whole blood results. Results: Validation and performance assessment tests tend toshow that most of the targeted elements provides accurate and reproducible results comparing to amethod of reference. Conclusion: Overall, VAMS presents good preliminary results to eventually becomean alternative to venipuncture for blood sampling for some trace elements analysis purposes.
DOI: 10.25549/examiner-c44-15387
title: Orphans party, 1951

description: 3 images. Orphans party, 10 August 1951. Bozo the Clown directs kids in balloon blowing contest.
DOI: 10.25402/bio.25146266
title: Validation and comprehensive analysis ofStreptococcus pneumoniae IgG WHOenzyme linked immunosorbent assay in anIndian reference laboratory: supplementary data

description: Monitoring serotype-specific IgG levels against pneumococci is crucial for assessing immunity, vaccineefficacy, and evaluating vaccination programs. The WHO ELISA for pneumococci is a standardized assayensuring consistency in testing and comparability of results across laboratories. It involves a rigoroustesting process to confirm accurate, precise, and reliable detection of antibodies. We validated theprotocol for 13 pneumococcal serotypes by assessing its specificity, reproducibility (coefficient of variation≤15%), repeatability (coefficient of variation ≤20%) , accuracy, lower limit of quantification, stability,and robustness. We found these parameters were within acceptable ranges and showed excellentperformance. Our findings imply that the method employed is appropriate for evaluating 13 valentpneumococcal conjugate vaccine which is introduced in the national immunization program by comparingpre-and post-vaccination IgG response.
DOI: 10.25402/bio.25146266.v1
title: Validation and comprehensive analysis ofStreptococcus pneumoniae IgG WHOenzyme linked immunosorbent assay in anIndian reference laboratory: supplementary data

description: Monitoring serotype-specific IgG levels against pneumococci is crucial for assessing immunity, vaccineefficacy, and evaluating vaccination programs. The WHO ELISA for pneumococci is a standardized assayensuring consistency in testing and comparability of results across laboratories. It involves a rigoroustesting process to confirm accurate, precise, and reliable detection of antibodies. We validated theprotocol for 13 pneumococcal serotypes by assessing its specificity, reproducibility (coefficient of variation≤15%), repeatability (coefficient of variation ≤20%) , accuracy, lower limit of quantification, stability,and robustness. We found these parameters were within acceptable ranges and showed excellentperformance. Our findings imply that the method employed is appropriate for evaluating 13 valentpneumococcal conjugate vaccine which is introduced in the national immunization program by comparingpre-and post-vaccination IgG response.
DOI: 10.25549/examiner-c44-15143
title: Santa Suzanna [Susana] fire, 1951

description: 6 images. Santa Suzanna [Susana] fire, 7 August 1951.; Caption slip reads: "Photographer: Snow. Assignment: Santa Suzanna Fire. 57-10: General view of Suzanna Knolls area burning. 58-9: General view of firemen and fire. 23-24: General view of fire in black canyon".
DOI: 10.25402/bio.25196069
title: Interlaboratory evaluation of LC–MS-basedbiomarker assays: supplementary materials

description: Validation of biomarker assays is crucial for effective drug development and clinical applications.Interlaboratory reproducibility is vital for reliable comparison and combination of data from differentcenters. This review summarizes interlaboratory studies of quantitative LC–MS-based biomarker assaysusing reference standards for calibration curves. The following points are discussed: trends in reports,reference and internal standards, evaluation of analytical validation parameters, study sample analysisand normalization of biomarker assay data. Full evaluation of these parameters in interlaboratory studiesis limited, necessitating further research. Some reports suggest methods to address variations in biomarkerassay data among laboratories, facilitating organized studies and data combination. Method validationacross laboratories is crucial for reducing interlaboratory differences and reflecting target biomarkerresponses.
DOI: 10.25402/bio.25196069.v1
title: Interlaboratory evaluation of LC–MS-basedbiomarker assays: supplementary materials

description: Validation of biomarker assays is crucial for effective drug development and clinical applications.Interlaboratory reproducibility is vital for reliable comparison and combination of data from differentcenters. This review summarizes interlaboratory studies of quantitative LC–MS-based biomarker assaysusing reference standards for calibration curves. The following points are discussed: trends in reports,reference and internal standards, evaluation of analytical validation parameters, study sample analysisand normalization of biomarker assay data. Full evaluation of these parameters in interlaboratory studiesis limited, necessitating further research. Some reports suggest methods to address variations in biomarkerassay data among laboratories, facilitating organized studies and data combination. Method validationacross laboratories is crucial for reducing interlaboratory differences and reflecting target biomarkerresponses.
DOI: 10.25402/bio.25196105
title: UHPLC-MS/MS for plasma lamotrigineanalysis and comparison with a homogenousenzyme immunoassay: supplementary data

description: Aims: To develop and validate a UHPLC-MS/MS method for lamotrigine (LTG) analysis in human plasmaand evaluate its agreement with a homogenous enzyme immunoassay (HEIA). Materials & methods: TheUHPLC-MS/MS method was developed and validated according to the USFDA/EMA guidelines. A Bland–Altman plot was used to evaluate the agreement between UHPLC-MS/MS and HEIA. Results: Samples werepretreated with one-step protein precipitation and separated in 2.6 min. The intra- and inter-day biasand imprecisions were -15.8 to 15.0% and less than 11.17%, respectively. The recovery and matrix factorwere 98.30 to 111.97%. The mean overestimation of UHPLC-MS/MS compared with HEIA was 21.57%.Conclusion: A rapid, sensitive and robust UHPLC-MS/MS method for plasma LTG analysis was developedand validated and was a 21.57% overestimation compared with HEIA.
DOI: 10.25402/bio.25196105.v1
title: UHPLC-MS/MS for plasma lamotrigineanalysis and comparison with a homogenousenzyme immunoassay: supplementary data

description: Aims: To develop and validate a UHPLC-MS/MS method for lamotrigine (LTG) analysis in human plasmaand evaluate its agreement with a homogenous enzyme immunoassay (HEIA). Materials & methods: TheUHPLC-MS/MS method was developed and validated according to the USFDA/EMA guidelines. A Bland–Altman plot was used to evaluate the agreement between UHPLC-MS/MS and HEIA. Results: Samples werepretreated with one-step protein precipitation and separated in 2.6 min. The intra- and inter-day biasand imprecisions were -15.8 to 15.0% and less than 11.17%, respectively. The recovery and matrix factorwere 98.30 to 111.97%. The mean overestimation of UHPLC-MS/MS compared with HEIA was 21.57%.Conclusion: A rapid, sensitive and robust UHPLC-MS/MS method for plasma LTG analysis was developedand validated and was a 21.57% overestimation compared with HEIA.
DOI: 10.25402/bio.25196240
title: Strategy and validation of a nonclinicalgeneric plug-and-play antidrug antibodymethod for human monoclonal antibodybiotherapeutics: supplementary tables

description: The measurement of antidrug antibodies (ADA) in nonclinical studies provides limited value because theformation and incidence of nonclinical ADA does not translate to clinical experience. The formation andpresence of ADA in nonclinical species can, however, correlate to reduced drug exposure and safetyobservations including vasculitis and immune complex disease. Generic ADA methods for humanizedmonoclonal antibody biotherapeutics mitigate the need to develop bespoke ADA methods duringnonclinical drug development. A drug-tolerant, sensitive, generic ADA immunoassay has been developedand validated for measuring ADA in cynomolgus monkey serum samples, allowing for immediatequalification of future monoclonal antibody biotherapeutics. This approach allows us to differentiatecomplexed and free ADA in a rapidly deployable manner when needed.
DOI: 10.25402/bio.25196240.v1
title: Strategy and validation of a nonclinicalgeneric plug-and-play antidrug antibodymethod for human monoclonal antibodybiotherapeutics: supplementary tables

description: The measurement of antidrug antibodies (ADA) in nonclinical studies provides limited value because theformation and incidence of nonclinical ADA does not translate to clinical experience. The formation andpresence of ADA in nonclinical species can, however, correlate to reduced drug exposure and safetyobservations including vasculitis and immune complex disease. Generic ADA methods for humanizedmonoclonal antibody biotherapeutics mitigate the need to develop bespoke ADA methods duringnonclinical drug development. A drug-tolerant, sensitive, generic ADA immunoassay has been developedand validated for measuring ADA in cynomolgus monkey serum samples, allowing for immediatequalification of future monoclonal antibody biotherapeutics. This approach allows us to differentiatecomplexed and free ADA in a rapidly deployable manner when needed.
DOI: 10.25549/examiner-c44-9589
title: Trout fishing (west fork in San Gabriel River), 1951

description: 12 images. Trout fishing (west fork in San Gabriel River), April 28, 1951. Randy Cobb; Michael Brunette; Kent Glasglow; Mrs. Hazel Mack; Mrs. Eunice Ward.
DOI: 10.25402/bio.25196255
title: A high-speed microscopy system based ondeep learning to detect yeast-like fungi cellsin blood: supplementary materials

description: Background: Blood-invasive fungal infections can cause the death of patients, while diagnosis of fungalinfections is challenging. Methods: A high-speed microscopy detection system was constructed thatincluded a microfluidic system, a microscope connected to a high-speed camera and a deep learninganalysis section. Results: For training data, the sensitivity and specificity of the convolutional neuralnetwork model were 93.5% (92.7–94.2%) and 99.5% (99.1–99.5%), respectively. For validating data, thesensitivity and specificity were 81.3% (80.0–82.5%) and 99.4% (99.2–99.6%), respectively. Cryptococcalcells were found in 22.07% of blood samples. Conclusion: This high-speed microscopy system can analyzefungal pathogens in blood samples rapidly with high sensitivity and specificity and can help dramaticallyaccelerate the diagnosis of fungal infectious diseases.
DOI: 10.25402/bio.25196255.v1
title: A high-speed microscopy system based ondeep learning to detect yeast-like fungi cellsin blood: supplementary materials

description: Background: Blood-invasive fungal infections can cause the death of patients, while diagnosis of fungalinfections is challenging. Methods: A high-speed microscopy detection system was constructed thatincluded a microfluidic system, a microscope connected to a high-speed camera and a deep learninganalysis section. Results: For training data, the sensitivity and specificity of the convolutional neuralnetwork model were 93.5% (92.7–94.2%) and 99.5% (99.1–99.5%), respectively. For validating data, thesensitivity and specificity were 81.3% (80.0–82.5%) and 99.4% (99.2–99.6%), respectively. Cryptococcalcells were found in 22.07% of blood samples. Conclusion: This high-speed microscopy system can analyzefungal pathogens in blood samples rapidly with high sensitivity and specificity and can help dramaticallyaccelerate the diagnosis of fungal infectious diseases.
DOI: 10.25549/examiner-c44-10992
title: Citizenship, 1951

description: 4 images. Citizenship, 27 July 1951. Mrs. Donald M. Nelson (Valerie); Mrs. June Hillman (formerly Lady Inverclyde).
DOI: 10.25402/bio.25196285
title: Development, validation and application ofan ion-pair reversed-phase liquidchromatography-tandem massspectrometry method for the quantificationof nusinersen: supplementary materials

description: Background: The fully phosphorothioate-modified oligonucleotide (OGN) nusinersen has low ionizationefficiency in the negative ion mode, resulting in a low mass spectrometry response. There havebeen no relevant reports on developing a LC–MS method for the determination of nusinersen byoptimizing mobile phase composition. Materials & methods: Mobile phase additives comprised of 15 mMtriethylamine/25 mM 1,1,1,3,3,3-hexafluoro-2-propanol with a pH of 9.6. Nusinersen was extracted fromplasma using Oasis R ? HLB solid-phase extraction (Waters, MA, USA). Results & conclusion: By adjustingthe pH of the mobile phase to 9.6 by optimizing the type and concentration of ion-pair reagents, a highmass spectrometry response was obtained. The developed method was applied to nusinersen and met therequirements for the pharmacokinetic study of nusinersen in rabbits.
DOI: 10.25402/bio.25196285.v1
title: Development, validation and application ofan ion-pair reversed-phase liquidchromatography-tandem massspectrometry method for the quantificationof nusinersen: supplementary materials

description: Background: The fully phosphorothioate-modified oligonucleotide (OGN) nusinersen has low ionizationefficiency in the negative ion mode, resulting in a low mass spectrometry response. There havebeen no relevant reports on developing a LC–MS method for the determination of nusinersen byoptimizing mobile phase composition. Materials & methods: Mobile phase additives comprised of 15 mMtriethylamine/25 mM 1,1,1,3,3,3-hexafluoro-2-propanol with a pH of 9.6. Nusinersen was extracted fromplasma using Oasis R ? HLB solid-phase extraction (Waters, MA, USA). Results & conclusion: By adjustingthe pH of the mobile phase to 9.6 by optimizing the type and concentration of ion-pair reagents, a highmass spectrometry response was obtained. The developed method was applied to nusinersen and met therequirements for the pharmacokinetic study of nusinersen in rabbits.
DOI: 10.15468/dl.g4smpz
title: Occurrence Download

description: A dataset containing 689 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Specimen, Human Observation)",
 "Country is Mexico",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Juniperus monticola Martínez"
 ]
}

 The dataset includes 689 records from 52 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0018198-240506114902167/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/examiner-c44-11446
title: Strike...Western mechanics union local 700, international mine, mill, and smelter workers, 1951

description: 4 images. Strike...Western mechanics union local 700, international mine, mill, and smelter workers, June 29, 1951. Picket line at Andrson Die Casting and Engineering Corporation at 1000 East 60th Street; Picket line at Union Die Casting Company Limited; Dorris Prosser -- 33 years (drill press operator); Ben Behmer.
DOI: 10.15468/dl.patpfk
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Guatemala",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Lycaste virginalis (Scheidw.) Linden"
 ]
}

 The dataset includes 15 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0018199-240506114902167/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.