DOI: 10.22002/0tms7-wq916
title: AimB depletion control w/ xylose

description: Raw data files of AimB depletion control w/ xylose
description: <strong>Tilt Series Date:</strong> 2009-09-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> The AimB_depletion strain was constructed by John Werner, data collected by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-30/rawdata/</p>09sep21a_AimBxyl_048.mrc,  Tilt Series (Pixel Size 1.192 nm), 536.9 MB  
        
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        09sep21a_AimBxyl_0481.tif,  2.6 MB  
        
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        09sep21a_AimBxyl_0482.tif,  1.7 MB  
        
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        09sep21a_AimBxyl_0483.tif,  2.6 MB  
        
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        09sep21a_AimBxyl_0484.tif,  2.6 MB  
        
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        09sep21a_AimBxyl_0485.tif,  1.7 MB  
        
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        09sep21a_AimBxyl_048.tif,  1.1 MB  
        
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