cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5885773.v1
title: Additional file 2: Table S1. of Computational master-regulator search reveals mTOR and PI3K pathways responsible for low sensitivity of NCI-H292 and A427 lung cancer cell lines to cytotoxic action of p53 activator Nutlin-3

description: Normalized expression values of all genes with detected expression in the studies conditions and mapped to Ensembl. In the tab â Nsen vs Senâ we give the results of Limma analysis of the LogFC between Nutlin-3 insensitive (Nsen) and sensitive cell lines. (XLSX 3291Â kb)
DOI: 10.6084/m9.figshare.5885758
title: Additional file 3: Table S3. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Criterion 1 results. (XLSX 59Â kb)
DOI: 10.6084/m9.figshare.5885758.v1
title: Additional file 3: Table S3. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Criterion 1 results. (XLSX 59Â kb)
DOI: 10.6084/m9.figshare.5885746
title: Additional file 2: Table S1. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Lists of genes associated with asthma and hypertension. (XLSX 105Â kb)
DOI: 10.6084/m9.figshare.5885746.v1
title: Additional file 2: Table S1. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Lists of genes associated with asthma and hypertension. (XLSX 105Â kb)
DOI: 10.6084/m9.figshare.5885728
title: Additional file 1: Table S2. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Results of gene ontology enrichment analysis. (XLSX 280Â kb)
DOI: 10.6084/m9.figshare.5885728.v1
title: Additional file 1: Table S2. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Results of gene ontology enrichment analysis. (XLSX 280Â kb)
DOI: 10.6084/m9.figshare.5885710
title: Additional file 10: Table S10. of Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks

description: Total score results. (XLSX 34Â kb)
DOI: 10.6084/m9.figshare.5885680
title: Evaluation of collared anteaters (Tamandua tetradactyla) presented in a wildlife health reference center of São Paulo state, Brazil

description: Abstract Deforestation and road construction have inflicted negative effects on wildlife populations. To contribute to the comprehension and preservation of wildlife species, this study aimed to evaluate collared anteater presented to a reference center located in São Paulo state, Brazil. Furthermore, the locations where the animals were most frequently found were determined. Data on collared anteaters (Tamandua tetradactyla) examined at the Center for Medicine and Research in Wild Animals (CEMPAS) were evaluated retrospectively for a period of 13 years. A total of 46 collared anteaters was examined, comprised of 14 males, 10 females, and 22 without identifying information. On average, adult males weigh 4.41 kg while adult females weigh 4.95 kg. The most common admission circumstance was vehicular strikes (36.96%), followed by dog bites (21.74%); 23.91% were considered clinically healthy. Animal outcomes were 36.96% (n=17) reintroduced, 34.78% (n=16) fatalities, 15.22% (n=7) transferred to other institutions, and 6.52% (n=3) escaped from the enclosure; and 6.52% (n=3) presented no medical record information. The collared anteaters were found mainly in the municipal areas of Botucatu (21.73%), São Manuel (17.39%), Lençóis Paulista (6.52%), and Bauru (4.34%), with SP-300 being the main highway associated with the occurrences. In conclusion, the main cause of lesion in the collared anteaters evaluated in this study was vehicular strike that that led to death. However, including all collared anteaters at least half of them had a positive outcome.
DOI: 10.6084/m9.figshare.5885680.v1
title: Evaluation of collared anteaters (Tamandua tetradactyla) presented in a wildlife health reference center of São Paulo state, Brazil

description: Abstract Deforestation and road construction have inflicted negative effects on wildlife populations. To contribute to the comprehension and preservation of wildlife species, this study aimed to evaluate collared anteater presented to a reference center located in São Paulo state, Brazil. Furthermore, the locations where the animals were most frequently found were determined. Data on collared anteaters (Tamandua tetradactyla) examined at the Center for Medicine and Research in Wild Animals (CEMPAS) were evaluated retrospectively for a period of 13 years. A total of 46 collared anteaters was examined, comprised of 14 males, 10 females, and 22 without identifying information. On average, adult males weigh 4.41 kg while adult females weigh 4.95 kg. The most common admission circumstance was vehicular strikes (36.96%), followed by dog bites (21.74%); 23.91% were considered clinically healthy. Animal outcomes were 36.96% (n=17) reintroduced, 34.78% (n=16) fatalities, 15.22% (n=7) transferred to other institutions, and 6.52% (n=3) escaped from the enclosure; and 6.52% (n=3) presented no medical record information. The collared anteaters were found mainly in the municipal areas of Botucatu (21.73%), São Manuel (17.39%), Lençóis Paulista (6.52%), and Bauru (4.34%), with SP-300 being the main highway associated with the occurrences. In conclusion, the main cause of lesion in the collared anteaters evaluated in this study was vehicular strike that that led to death. However, including all collared anteaters at least half of them had a positive outcome.
DOI: 10.6084/m9.figshare.5885677
title: The use of information and communication technologies (ICTs) in health centers: the practititoners’ point of view in Catalonia, Spain

description: The aim of this research is to identify experiences using information and communication technologies (ICTs) that are able to improve the public management of health centers in Catalonia (Spain), as well as being generated bottom-up by primary health care practitioners. We have compiled these experiences through eleven semi-structured interviews with primary care physicians and nurses. The result is a set of experiences that can improve public management in health centers through a) greater interaction between patients and primary health care providers; b) more patient-centred medical care; and c) greater participation and involvement of patients in their own health situation.
DOI: 10.6084/m9.figshare.5885674
title: Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories

description: ABSTRACT Introduction: The detection of autoantibodies in HEp-2 cells represents a relevant tool for the diagnosis of autoimmune diseases, especially rheumatic autoimmune diseases. As a result of the methodological advances, the technique gradually increased the sensitivity, as well as the need for standardization. Objective: To evaluate the implementation of the Brazilian Consensus recommendations for autoantibody determination in HEp-2 cells. Methods: A structured form in a virtual platform was filled in by experts in clinical laboratories that carry out the methodology across the country. The questionnaire addressed the adoption of the Brazilian Consensus guidelines, detailing the technical aspects, quality control, the strategy for reading slides and the release of reports. Results: The study included 53 laboratories responsible for more than 300,000 antinuclear antibody (ANA) tests/month; more than half (58.5%) reported fully adopting the recommendations of the Brazilian Consensus. The majority (83.1%) used the 1:80 for screening dilution, and 75.5% of laboratories, perform education and quality control programs. Only 39.6% reported using more than one kit brand to perform the test, and 32.1% did not report observing all phases of the cell cycle during slide reading. The study also detected some heterogeneity among participants in the identification of patterns. Conclusion: The results confirm the adoption of the Brazilian Consensus recommendations by most of participating laboratories, although with variable extent. There is need for improvement in some aspects, especially those related to the quality control.
DOI: 10.6084/m9.figshare.5885674.v1
title: Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories

description: ABSTRACT Introduction: The detection of autoantibodies in HEp-2 cells represents a relevant tool for the diagnosis of autoimmune diseases, especially rheumatic autoimmune diseases. As a result of the methodological advances, the technique gradually increased the sensitivity, as well as the need for standardization. Objective: To evaluate the implementation of the Brazilian Consensus recommendations for autoantibody determination in HEp-2 cells. Methods: A structured form in a virtual platform was filled in by experts in clinical laboratories that carry out the methodology across the country. The questionnaire addressed the adoption of the Brazilian Consensus guidelines, detailing the technical aspects, quality control, the strategy for reading slides and the release of reports. Results: The study included 53 laboratories responsible for more than 300,000 antinuclear antibody (ANA) tests/month; more than half (58.5%) reported fully adopting the recommendations of the Brazilian Consensus. The majority (83.1%) used the 1:80 for screening dilution, and 75.5% of laboratories, perform education and quality control programs. Only 39.6% reported using more than one kit brand to perform the test, and 32.1% did not report observing all phases of the cell cycle during slide reading. The study also detected some heterogeneity among participants in the identification of patterns. Conclusion: The results confirm the adoption of the Brazilian Consensus recommendations by most of participating laboratories, although with variable extent. There is need for improvement in some aspects, especially those related to the quality control.
DOI: 10.6084/m9.figshare.5885668
title: Frequencies of CD39, IVS1-1, IVS1-6 and IVS1-110 mutations in beta-thalassemia carriers and their influence on hematimetric indices

description: ABSTRACT Introduction: Beta-thalassemia is caused by a deficient synthesis of the ß-chain of hemoglobin, which leads to a chronic, microcytic and hypochromic anemia. More than 200 mutations have already been associated with this type of thalassemia, and their frequencies may vary according to the population. Objectives: The objectives of this study were to determine the frequencies of CD39, IVS1-1, IVS1-6 and IVS1-110 mutations in people with beta-thalassemia from the city of Franca, São Paulo, and to evaluate the influence of the genotypes on hematological alterations. Methods: Venous blood samples were collected from 25 volunteers previously diagnosed with beta-thalassemia. Complete blood counts (CBC) were performed, and the identification of the mutations was carried out using the polymerase chain reaction (PCR). Results: The CD39 mutation was found in 11 (44%) individuals, followed by IVS1-6 (9; 36%) and IVS1-110 (4; 16%). One patient (4%) did not present any of these mutations. IVS1-6 mutation was inversely correlated to red cell distribution width (RDW) (rs = -0.44; p = 0.034), and CD39 was correlated to lower mean corpuscular volume (MCV) (rs = -0.44; p = 0.034). Multivariable linear regression models showed that the CD39 mutation carriers have lower levels for hemoglobin (ß = -0.61; p = 0.044) and hematocrit (ß = -2.1; p = 0.018). Conclusion: The results showed a high frequency of the CD39 mutation in the city of Franca, and the correlations observed between the presence of CD39 mutation and the hematological alterations suggest a genotype influence on the phenotype of beta-thalassemia, which would contribute to the clinical variations of this hemoglobinopathy.
DOI: 10.6084/m9.figshare.5885668.v1
title: Frequencies of CD39, IVS1-1, IVS1-6 and IVS1-110 mutations in beta-thalassemia carriers and their influence on hematimetric indices

description: ABSTRACT Introduction: Beta-thalassemia is caused by a deficient synthesis of the ß-chain of hemoglobin, which leads to a chronic, microcytic and hypochromic anemia. More than 200 mutations have already been associated with this type of thalassemia, and their frequencies may vary according to the population. Objectives: The objectives of this study were to determine the frequencies of CD39, IVS1-1, IVS1-6 and IVS1-110 mutations in people with beta-thalassemia from the city of Franca, São Paulo, and to evaluate the influence of the genotypes on hematological alterations. Methods: Venous blood samples were collected from 25 volunteers previously diagnosed with beta-thalassemia. Complete blood counts (CBC) were performed, and the identification of the mutations was carried out using the polymerase chain reaction (PCR). Results: The CD39 mutation was found in 11 (44%) individuals, followed by IVS1-6 (9; 36%) and IVS1-110 (4; 16%). One patient (4%) did not present any of these mutations. IVS1-6 mutation was inversely correlated to red cell distribution width (RDW) (rs = -0.44; p = 0.034), and CD39 was correlated to lower mean corpuscular volume (MCV) (rs = -0.44; p = 0.034). Multivariable linear regression models showed that the CD39 mutation carriers have lower levels for hemoglobin (ß = -0.61; p = 0.044) and hematocrit (ß = -2.1; p = 0.018). Conclusion: The results showed a high frequency of the CD39 mutation in the city of Franca, and the correlations observed between the presence of CD39 mutation and the hematological alterations suggest a genotype influence on the phenotype of beta-thalassemia, which would contribute to the clinical variations of this hemoglobinopathy.
DOI: 10.6084/m9.figshare.5885665
title: COMPARATIVE STUDY OF THE APPRAISAL ATTITUDES OF OSCAR WILDE IN THE LETTER DE PROFUNDIS AND ITS TRANSLATION TO BRAZILIAN PORTUGUESE

description: ABSTRACT This paper is concerned with an investigation on the appraisal resources (MARTIN e ROSE, 2003; MARTIN e ROSE, 2007; MARTIN e WHITE, 2005; MUNDAY, 2012; RODRIGUES-JÚNIOR, 2016) used by Oscar Wilde in his manuscript De Profundis, in comparison with its translation into Brazilian portuguese. The letter, written on a given context of Wilde’s life, when he was arrested for having committed some acts considered crimes by the society of his time, presents many linguistic manifestation of evaluative nature. The aims of the study are: i) to verify how the lexical choices that express affect, judgement and appreciation appear on the source and target texts; ii) to contribute to the analysis of translations from a systemic functional perspective, especially texts that present evaluative attitudes; ii) to discuss the presence of the social subject Oscar Wilde in his letter De Profundis. The metodological procedures used were the comparison of the shifts of equivalence in terms of attitudinal evaluation (CATFORD, 1965; RODRIGUES-JÚNIOR; OLIVEIRA, 2015), the analysis of the shifts and the quantitative discussions concerning the shifts found in the corpus. It was possible to identify three kinds of shifts referring to the linguistic constructions of appraisal: the ones that do not modify the semantic meaning potential of the source text, the ones that modify the meaning potential and the omissions. The study has developed a new kind of equivalent shift category in the scope of appraisal attitudes, which is called Attitudinal Shifts.
DOI: 10.6084/m9.figshare.5885665.v1
title: COMPARATIVE STUDY OF THE APPRAISAL ATTITUDES OF OSCAR WILDE IN THE LETTER DE PROFUNDIS AND ITS TRANSLATION TO BRAZILIAN PORTUGUESE

description: ABSTRACT This paper is concerned with an investigation on the appraisal resources (MARTIN e ROSE, 2003; MARTIN e ROSE, 2007; MARTIN e WHITE, 2005; MUNDAY, 2012; RODRIGUES-JÚNIOR, 2016) used by Oscar Wilde in his manuscript De Profundis, in comparison with its translation into Brazilian portuguese. The letter, written on a given context of Wilde’s life, when he was arrested for having committed some acts considered crimes by the society of his time, presents many linguistic manifestation of evaluative nature. The aims of the study are: i) to verify how the lexical choices that express affect, judgement and appreciation appear on the source and target texts; ii) to contribute to the analysis of translations from a systemic functional perspective, especially texts that present evaluative attitudes; ii) to discuss the presence of the social subject Oscar Wilde in his letter De Profundis. The metodological procedures used were the comparison of the shifts of equivalence in terms of attitudinal evaluation (CATFORD, 1965; RODRIGUES-JÚNIOR; OLIVEIRA, 2015), the analysis of the shifts and the quantitative discussions concerning the shifts found in the corpus. It was possible to identify three kinds of shifts referring to the linguistic constructions of appraisal: the ones that do not modify the semantic meaning potential of the source text, the ones that modify the meaning potential and the omissions. The study has developed a new kind of equivalent shift category in the scope of appraisal attitudes, which is called Attitudinal Shifts.
DOI: 10.6084/m9.figshare.5885662
title: Jaburetox: update on a urease-derived peptide

description: Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes. A recombinant form of this peptide, called jaburetox (JBTX), was constructed using jburell gene as a template. The peptide exhibits liposome disruption properties, and insecticidal and fungicidal activities. Here we review the known biological properties activities of JBTX, and comment on new ones not yet fully characterized. JBTX was able to cause mortality of Aedes aegypti larvae in a feeding assay whereas in a dose as low as of 0.1 μg it provoked death of Triatoma infestans bugs. JBTX (10−5-10−6 M) inhibits the growth of E. coli, P. aeruginosa and B. cereus after 24 h incubation. Multilamellar liposomes interacting with JBTX undergo reorganization of the membrane's lipids as detected by small angle X-ray scattering (SAXS) studies. Encapsulating JBTX into lipid nanoparticles led to an increase of the peptide's antifungal activity. Transgenic tobacco and sugarcane plants expressing the insecticidal peptide JBTX, showed increased resistance to attack of the insect pests Spodoptera frugiperda, Diatraea saccharalis and Telchin licus licus. Many questions remain unanswered; however, so far, JBTX has shown to be a versatile peptide that can be used against various insect and fungus species, and in new bacterial control strategies.
DOI: 10.6084/m9.figshare.5885662.v1
title: Jaburetox: update on a urease-derived peptide

description: Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes. A recombinant form of this peptide, called jaburetox (JBTX), was constructed using jburell gene as a template. The peptide exhibits liposome disruption properties, and insecticidal and fungicidal activities. Here we review the known biological properties activities of JBTX, and comment on new ones not yet fully characterized. JBTX was able to cause mortality of Aedes aegypti larvae in a feeding assay whereas in a dose as low as of 0.1 μg it provoked death of Triatoma infestans bugs. JBTX (10−5-10−6 M) inhibits the growth of E. coli, P. aeruginosa and B. cereus after 24 h incubation. Multilamellar liposomes interacting with JBTX undergo reorganization of the membrane's lipids as detected by small angle X-ray scattering (SAXS) studies. Encapsulating JBTX into lipid nanoparticles led to an increase of the peptide's antifungal activity. Transgenic tobacco and sugarcane plants expressing the insecticidal peptide JBTX, showed increased resistance to attack of the insect pests Spodoptera frugiperda, Diatraea saccharalis and Telchin licus licus. Many questions remain unanswered; however, so far, JBTX has shown to be a versatile peptide that can be used against various insect and fungus species, and in new bacterial control strategies.
DOI: 10.6084/m9.figshare.5885653
title: Laboratory diagnosis of chronic kidney disease in adults: an overview of hospitals inserted in the Portuguese National Health System

description: ABSTRACT Introduction: The laboratory diagnosis of chronic kidney disease (CKD) is a simple and cost-effective procedure that allows the detection of early stages of the disease, which is essential to avoid kidney damage and a life threateaning event. It consists of measuring serum creatinine concentration, urinary albumin concentration and calculating the estimated glomerular filtration rate (eGFR). In 2012, the guidelines for laboratory evaluation of the CKD were published by the Kidney Disease: Improving Global Outcomes (KDIGO). Objectives: This study aimed to evaluate whether the laboratories in hospitals of the Portuguese National Health System follow these guidelines and provide a correct diagnosis of CKD. Material and method: A questionnaire composed of 32 questions was sent to the Clinical Pathology Services of all hospitals inserted in the System. Results: All 49 labs responded that measure serum creatinine, 18 reported measurering eGFR. Ten reported measuring eGFR only if specifically ordered. Forty-four measure total protein and albumin in the urine, three only protein, one albumin alone, and one measure none of them. The type of samples, methods, reagents, equipment, expression units of results and reference intervals varied. Conclusion: There is great variability among laboratories in relation to the methodology of measuring serum creatinine, albumin and total protein in the urine. There are wide variations in the release of results. Most laboratories do not follow the guidelines recommended by the KDIGO 2012. This work indicates that there is a need to develop education and alignment processes in the laboratory diagnosis of CKD in the laboratories installed in hospitals inserted in the Portuguese National Health System.