cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30019309.v1
title: Autotaxin, LPA receptor and ECM RNA expression in Normal vs Glaucoma Lamina Cribrosa Cells

description: Lamina cribrosa (LC) cells from age-matched glaucoma and normal control patient donors were cultured. RNA was extracted from fresh LC cells by adding 1ml of TriReagent to cell culture flasks and transferring to a 1.5ml Eppendorf. 100μl chloroform was added and incubated on ice for 5 minutes before centrifuging at 12,000 RPM. 500μl isopropanol was added to the aqueous layer, incubated for 5 minutes on ice and centrifuged at 12,000 RPM. The supernatant was discarded. 1ml ethanol was added to the pellet, centrifuged, and discarded. The airdried pellet was resuspended in 10-20μl diethylpyrocarbonate treated water. RNA yield and quality was confirmed by nanodrop. 2-3 nanograms of RNA was converted to complimentary DNA by adding 1.5μl oligoDTs, and DEPC water to make a total volume 13μl. Samples were heated to 65°C for 5 minutes. 2μl deoxynucleotides were added to the samples, with 1μl RevertAid Reverse Transcriptase and 4μl 5x Reverse Transcriptase Buffer. This total volume of 20μl was incubated in a thermocycler at 45°C for 60 minutes and 70°C for 10 minutes. 1μl cDNA was added to wells of a white 96 well PCR plate in triplicate. 5μl SYBR GREEN, 0.5μl forward primer, 0.5μl reverse primer and 3μl DEPC water were added to each sample. Primer sequences were designed using prior studies and the National Center for Biotechnology Information’s Primer-BLAST tool. Primers were acquired from Sigma-Aldrich. 18S was used as a housekeeping gene. The LightCycler® 480 Instrument II (Roche) was used, and standard PCR cycles were as follows: Denaturation at 95°C for 5 min, denaturation at 95°C for 10 seconds, annealing at 60°C for 20 seconds, elongation at 72°C for 20 seconds. This sequence was repeated for 45 cycles from the second denaturation step to amplify the product, followed by a melt curve of 95°C for 5 seconds and 65°C for 1 minute. Relative change in gene expression was calculated using the 2-DDCt equation. LC cells of the same passage from the three normal donors and the three glaucoma donors were used for each experiment, with technical triplicates for each donor.
DOI: 10.6084/m9.figshare.30019309
title: Autotaxin, LPA receptor and ECM RNA expression in Normal vs Glaucoma Lamina Cribrosa Cells

description: Lamina cribrosa (LC) cells from age-matched glaucoma and normal control patient donors were cultured. RNA was extracted from fresh LC cells by adding 1ml of TriReagent to cell culture flasks and transferring to a 1.5ml Eppendorf. 100μl chloroform was added and incubated on ice for 5 minutes before centrifuging at 12,000 RPM. 500μl isopropanol was added to the aqueous layer, incubated for 5 minutes on ice and centrifuged at 12,000 RPM. The supernatant was discarded. 1ml ethanol was added to the pellet, centrifuged, and discarded. The airdried pellet was resuspended in 10-20μl diethylpyrocarbonate treated water. RNA yield and quality was confirmed by nanodrop. 2-3 nanograms of RNA was converted to complimentary DNA by adding 1.5μl oligoDTs, and DEPC water to make a total volume 13μl. Samples were heated to 65°C for 5 minutes. 2μl deoxynucleotides were added to the samples, with 1μl RevertAid Reverse Transcriptase and 4μl 5x Reverse Transcriptase Buffer. This total volume of 20μl was incubated in a thermocycler at 45°C for 60 minutes and 70°C for 10 minutes. 1μl cDNA was added to wells of a white 96 well PCR plate in triplicate. 5μl SYBR GREEN, 0.5μl forward primer, 0.5μl reverse primer and 3μl DEPC water were added to each sample. Primer sequences were designed using prior studies and the National Center for Biotechnology Information’s Primer-BLAST tool. Primers were acquired from Sigma-Aldrich. 18S was used as a housekeeping gene. The LightCycler® 480 Instrument II (Roche) was used, and standard PCR cycles were as follows: Denaturation at 95°C for 5 min, denaturation at 95°C for 10 seconds, annealing at 60°C for 20 seconds, elongation at 72°C for 20 seconds. This sequence was repeated for 45 cycles from the second denaturation step to amplify the product, followed by a melt curve of 95°C for 5 seconds and 65°C for 1 minute. Relative change in gene expression was calculated using the 2-DDCt equation. LC cells of the same passage from the three normal donors and the three glaucoma donors were used for each experiment, with technical triplicates for each donor.
DOI: 10.6084/m9.figshare.30019315
title: In Silico Modeled Structure of Blinatumomab (Bispecific T-cell Engager)

description: This file contains the predicted three-dimensional structure of the bispecific T-cell engager (BiTE) blinatumomab, computationally constructed in silico by linking anti-CD19 and anti-CD3 single-chain variable fragments (scFvs) with flexible GGGGS peptide linkers. The structure was generated using AlphaFold2-multimer via ColabFold and refined through molecular dynamics simulations in GROMACS. The model is provided in PDB format for visualization, analysis, and further computational studies.<br>
DOI: 10.6084/m9.figshare.30019315.v1
title: In Silico Modeled Structure of Blinatumomab (Bispecific T-cell Engager)

description: This file contains the predicted three-dimensional structure of the bispecific T-cell engager (BiTE) blinatumomab, computationally constructed in silico by linking anti-CD19 and anti-CD3 single-chain variable fragments (scFvs) with flexible GGGGS peptide linkers. The structure was generated using AlphaFold2-multimer via ColabFold and refined through molecular dynamics simulations in GROMACS. The model is provided in PDB format for visualization, analysis, and further computational studies.<br>
DOI: 10.22002/6r2dg-49736
title: Agro C58 22C liq

description: Raw data files of Agro C58 22C liq
description: <strong>Tilt Series Date:</strong> 2014-08-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> C58 from Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 22C liquid</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-08-05-22/rawdata/</p>agro_22_liq10003.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-08-05-22/rawdata/agro_22_liq10003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vy8t0-txx22
title: Agrobacterium tumefaciens

description: Agrobacterium_VirB8-GFP_deltaVirB8
description: <strong>Tilt Series Date:</strong> 2013-05-07</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from PatZambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Titan_K2</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-45/rawdata/</p>VirB_deltaVirB0015.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-05-07-45/rawdata/VirB_deltaVirB0015.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.17012954
title: A text book of Linear Algebra and Matrices

description: This book, A Textbook of Linear Algebra and Matrices, is written to meet the requirements of B.A. and B.Sc. students under the recommended unified syllabus of Mathematics. It is designed as a complete text for formal courses in Linear Algebra and Matrices. The book is divided into two parts: the first part covers Linear Algebra, introducing all necessary definitions and concepts clearly. The second part focuses on Matrices, following the syllabus closely. Each topic is explained in a simple and systematic manner to aid understanding. We hope the book will be a valuable resource for both students and teachers.
DOI: 10.5281/zenodo.17012955
title: A text book of Linear Algebra and Matrices

description: This book, A Textbook of Linear Algebra and Matrices, is written to meet the requirements of B.A. and B.Sc. students under the recommended unified syllabus of Mathematics. It is designed as a complete text for formal courses in Linear Algebra and Matrices. The book is divided into two parts: the first part covers Linear Algebra, introducing all necessary definitions and concepts clearly. The second part focuses on Matrices, following the syllabus closely. Each topic is explained in a simple and systematic manner to aid understanding. We hope the book will be a valuable resource for both students and teachers.
DOI: 10.22002/dv1a2-9sz45
title: Agro_C58_22C_liq

description: Raw data files of Agro_C58_22C_liq
description: <strong>Tilt Series Date:</strong> 2014-09-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 22C liquid</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-09-05-31/rawdata/</p>c58_22_liq20004.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-09-05-31/rawdata/c58_22_liq20004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a4egz-tcs40
title: M. xanthus pilA - pilY1.3-flag

description: Raw data files of M. xanthus pilA - pilY1.3-flag
description: <strong>Tilt Series Date:</strong> 2020-01-31</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Yi-Wei Chang, Anke Treuner-Lange, Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated 15 mL 1% CTT with 1mL previously frozen glycerol stock. . Incubated at 32C/225rpm for 2 days.</p>
<strong>Sample Preparation:</strong> Harvested, mixed 40uL cells with 10nm Au fiducials, plunge froze 4uL . (bf 6, bt 6s, d 1s)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-13/rawdata/</p>pilY13_2_018_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-13/rawdata/pilY13_2_018_af.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/875bw-rce05
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-11-14</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, WT</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-43/rawdata/</p>targ43.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-11-14-43/rawdata/targ43.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eczg0-7h384
title: M. xanthus pilA - pilY1.3-flag

description: Raw data files of M. xanthus pilA - pilY1.3-flag
description: <strong>Tilt Series Date:</strong> 2020-01-31</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Yi-Wei Chang, Anke Treuner-Lange, Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated 15 mL 1% CTT with 1mL previously frozen glycerol stock. . Incubated at 32C/225rpm for 2 days.</p>
<strong>Sample Preparation:</strong> Harvested, mixed 40uL cells with 10nm Au fiducials, plunge froze 4uL . (bf 6, bt 6s, d 1s)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-25/rawdata/</p>pilY13_2_036_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-25/rawdata/pilY13_2_036_af.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.17012981
title: English interiors 263-272
DOI: 10.5281/zenodo.17012982
title: English interiors 263-272
DOI: 10.22002/jv73w-p5810
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-355/rawdata/</p>JV9298_PD-1-3_327.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-355/rawdata/JV9298_PD-1-3_327.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kbqcy-jak58
title: B. diazoefficiens, hopanoids

description: WT strain, stationary phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 70.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase</p>
<strong>Sample Preparation:</strong> Broad vitrobot, blot force 6, blot time 3s, drain 1s, wait 0s, 22 degC, 100% humidity.. 4uL of 6X concentrated cell (original O.D. 1.2). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-4/rawdata/</p>WT_ST_70step1p5_3.mrc,  Tilt Series (Pixel Size 2.678 nm), 4.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-4/rawdata/WT_ST_70step1p5_3.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2jy0j-3c709
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-05</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-84/rawdata/</p>targ84.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-84/rawdata/targ84.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hvu2mw
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Paspalum paniculatum",
 "Country is Vanuatu",
 "HasCoordinate is true"
 ]
}

 The dataset includes 8 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013678-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/hxyp0-fjv26
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-35/rawdata/</p>AnchorHM.mrc,  Tilt Series (Pixel Size 0.39 nm), 86.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-35/rawdata/AnchorHM.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wh4za-zd350
title: Pombe Cryosections

description: These cells were high pressure frozen after the majority of the rings were around mid-division.
description: <strong>Tilt Series Date:</strong> 2015-06-03</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Manual/Raptor</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-06-03-20/rawdata/</p>060315_pombe_cryosection2_square6_atlas.mrc,  Tilt Series (Pixel Size 0.5 nm), 4.3 MB  
        
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        > <i class="download icon"></i> Download </a></p>