DOI: 10.5061/dryad.13bd2
title: Data from: Assessing early fitness consequences of exotic gene flow in the wild: a field study with Iberian pine relicts

description: Gene flow from plantations of nonlocal (genetically exotic) tree
 provenances into natural stands of the same species is probably a
 widespread phenomenon, but its effects remain largely unexamined. We
 investigated early fitness consequences of intraspecific exotic gene flow
 in the wild by assessing differences in survival among native, nonlocal,
 and F1 intraspecific hybrid seedlings naturally established within two
 native pine relicts (one of Pinus pinaster and the other of P. sylvestris)
 surrounded by nonlocal plantations. We obtained broad-scale temporally
 sequential genotypic samples of a cohort of recruits in each pine relict,
 from seeds before dispersal to established seedlings months after
 emergence, tracking temporal changes in the estimated proportion of each
 parental cross-type. Results show significant proportions of exotic male
 gametes before seed dispersal in the two pine relicts. Subsequently to
 seedling establishment, the frequency of exotic male gametes became
 nonsignificant in P. pinaster, and dropped by half in P. sylvestris.
 Exotic zygotic gene flow was significantly different from zero among early
 recruits for P. sylvestris, decreasing throughout seedling establishment.
 Seedling mortality resulted in small late sample sizes, and temporal
 differences in exotic gene flow estimates were not significant, so we
 could not reject the null hypothesis of invariant early viability across
 parental cross types in the wild.
description: Nuclear and chloroplast
 microsatellite genotypes of open-pollinated seeds and naturally
 established seedlings in Pinus pinaster at Fuencaliente (Ciudad Real,
 Spain)One line per combined
 nuclear-chloroplast individual genotype, comprising 12 nSSR diploid loci
 and 6 cpSSR haploid regions. For nSSR, the two alleles at each locus are
 listed in consecutive columns. Missing data coded with zeros. Samples
 collected in the field. DNA extracted with Invisorb DNA Plant HTS 96 Kit.
 Electrophoresis of amplified products with LI-COR 4300 DNA Analyzer for
 cpSSRs, and 3130xL and 3500 Genetic Analyzers (Applied Biosystems) for
 nSSRs. Fragment size determination through visual inspection using
 external size markers and reference samples with known allele size for
 cpSSR, and internal size standard and GeneMapper software (Applied
 Biosystems) for nSSR. The reference adult genotypes used in the study are
 available at http://dx.doi.org/10.5061/dryad.pq6hg.Ppinaster_Fuencaliente_cp&nSSRs.csvNuclear and chloroplast microsatellite genotypes of open-pollinated seeds and naturally established seedlings in Pinus sylvestris at Sierra Nevada (Granada, Spain)One line per combined nuclear-chloroplast individual genotype, comprising 10 nSSR diploid loci and 7 cpSSR haploid regions. For nSSR, the two alleles at each locus are listed in consecutive columns. Missing data coded with zeros. Samples collected in the field. DNA extracted with Invisorb DNA Plant HTS 96 Kit. Electrophoresis of amplified products with LI-COR 4300 DNA Analyzer for cpSSRs, and 3130xL and 3500 Genetic Analyzers (Applied Biosystems) for nSSRs. Fragment size determination through visual inspection using external size markers and reference samples with known allele size for cpSSR, and internal size standard and GeneMapper software (Applied Biosystems) for nSSR. The reference adult genotypes used in the study are available at http://dx.doi.org/10.5061/dryad.pq6hgPsylvestris_SierraNevada_cp&nSSRs.csv