cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8848304.v1
title: Evaluation of muscle tissue and liver glycogen of cattle submitted to transport over long distances and subjected to emergency slaughter

description: ABSTRACT The study evaluated the effect of transportation over long distances on cattle muscle tissue of submitted to emergency slaughter in slaughterhouses in northern Tocantins, Brazil. The evaluations consisted in pH, muscle and liver glycogen, muscle histopathology and creatine kinase (CK), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity. Animals were placed into two groups: Experimental Group (EG), consisting of 19 bovines intended for immediate emergency slaughter, and Control Group (CG), composed of 24 bovines slaughtered in accordance with the normal flow. CK and ALT levels were high in EG. AST did not differ between groups. EG showed higher muscle pH and mean of degenerate fibers, mainly on the intercostal. However, muscle and liver glycogen did not differ between groups. In conclusion, cattle transported over long distances and subjected to immediate emergency slaughter showed markedly stress condition, with changes in biochemical parameters in the muscle tissue, determined by cellular degeneration.
DOI: 10.6084/m9.figshare.8848295
title: Pagetoid reticulosis in a dog: case report

description: ABSTRACT Reticulosis pagetoide is an expression used for one of the rare forms of lymphoma described in dogs. It is a cutaneous lymphoproliferative disorder that emerges from intraepidermal T-cells. We describe a case of pagetoid reticulosis with localized presentation, very similar to Woringer-Kolopp's disease in humans, which affected a 10-year-old Boxer female. The patient was seen due to the appearance of a nodule in the nasolabial region with clinical evolution of about 30 days. Histologically, it was composed of an intraepidermal and annexal lymphoid proliferation. Neoplastic lymphocytes, T-cells confirmed by CD3-positive and CD79-negative immunolabeling, were large, round, and monomorphic cells. Although rare, pagetoid reticulosis is an easily suspected lymphoproliferative disorder based only on cell morphology. This neoplasm should be remembered whenever an intraepidermal lymphoid pattern is present.
DOI: 10.6084/m9.figshare.8848292
title: Effects of inspired oxygen fractions in rabbits anesthetized with isoflurane or sevoflurane, maintained on spontaneous ventilation

description: ABSTRACT It is important to identify the best inspired fraction of oxygen in a variety of situations, including sevoflurane or isoflurane anesthesia, in spontaneously breathing rabbits. For this, 64 rabbits were assigned to eight groups: GI100 (FiO2= 1,0 + isoflurane), GS100 (FiO2= 1,0 + sevoflurane), GI80 (FiO2= 0,8 + isoflurane), GS80 (FiO2= 0,8 + sevoflurane), GI60 (FiO2= 0,6 + isoflurane), GS60 (FiO2= 0,6 + sevoflurane), GI21 (FiO2= 0,21 + isoflurane), GS21 (FiO2= 0,21 + sevoflurane). The induction was performed with (2.5MAC) of the anesthetic. The vaporizer was setted at 1.5 MAC and FiO2 as attributed for each group. After the induction, the concentration was changed to 1 MAC. Measurements of parameters were performed 30 minutes after induction (T0), and then at 15 minute intervals (from T15 to T60). The arterial partial pressures of oxygen (PaO2), alveolar oxygen partial pressure (PAO2) and alveolar-arterial oxygen gradient [P(A-a)O2] were higher with the use of high FiO2. The GI80 showed higher levels of PaO2 FiO2 ratio and respiratory index (RI). In conclusion, the FiO2 of 0.21 is not indicated, because it causes hypoxemia. The isoflurane determines better ventilation when compared to sevoflurane, but isoflurane associated with 80% of oxygen promotes intrapulmonary shunt increase.
DOI: 10.6084/m9.figshare.8848292.v1
title: Effects of inspired oxygen fractions in rabbits anesthetized with isoflurane or sevoflurane, maintained on spontaneous ventilation

description: ABSTRACT It is important to identify the best inspired fraction of oxygen in a variety of situations, including sevoflurane or isoflurane anesthesia, in spontaneously breathing rabbits. For this, 64 rabbits were assigned to eight groups: GI100 (FiO2= 1,0 + isoflurane), GS100 (FiO2= 1,0 + sevoflurane), GI80 (FiO2= 0,8 + isoflurane), GS80 (FiO2= 0,8 + sevoflurane), GI60 (FiO2= 0,6 + isoflurane), GS60 (FiO2= 0,6 + sevoflurane), GI21 (FiO2= 0,21 + isoflurane), GS21 (FiO2= 0,21 + sevoflurane). The induction was performed with (2.5MAC) of the anesthetic. The vaporizer was setted at 1.5 MAC and FiO2 as attributed for each group. After the induction, the concentration was changed to 1 MAC. Measurements of parameters were performed 30 minutes after induction (T0), and then at 15 minute intervals (from T15 to T60). The arterial partial pressures of oxygen (PaO2), alveolar oxygen partial pressure (PAO2) and alveolar-arterial oxygen gradient [P(A-a)O2] were higher with the use of high FiO2. The GI80 showed higher levels of PaO2 FiO2 ratio and respiratory index (RI). In conclusion, the FiO2 of 0.21 is not indicated, because it causes hypoxemia. The isoflurane determines better ventilation when compared to sevoflurane, but isoflurane associated with 80% of oxygen promotes intrapulmonary shunt increase.
DOI: 10.6084/m9.figshare.8848295.v1
title: Pagetoid reticulosis in a dog: case report

description: ABSTRACT Reticulosis pagetoide is an expression used for one of the rare forms of lymphoma described in dogs. It is a cutaneous lymphoproliferative disorder that emerges from intraepidermal T-cells. We describe a case of pagetoid reticulosis with localized presentation, very similar to Woringer-Kolopp's disease in humans, which affected a 10-year-old Boxer female. The patient was seen due to the appearance of a nodule in the nasolabial region with clinical evolution of about 30 days. Histologically, it was composed of an intraepidermal and annexal lymphoid proliferation. Neoplastic lymphocytes, T-cells confirmed by CD3-positive and CD79-negative immunolabeling, were large, round, and monomorphic cells. Although rare, pagetoid reticulosis is an easily suspected lymphoproliferative disorder based only on cell morphology. This neoplasm should be remembered whenever an intraepidermal lymphoid pattern is present.
DOI: 10.6084/m9.figshare.8848283
title: Thermal, dielectric and catalytic behavior of palladium doped PVC films

description: Abstract The present paper discusses the aspects of synthesizing palladium (Pd) doped (Pd+2 and Pd0) poly(vinyl chloride) (PVC) using simple solution cast technique. The Pd loading to PVC was altered from 2.5% to 10.0% and the material properties were studied using UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDX) and Field Emission Scanning Electron Microscopy (FE-SEM). Thermal behavior of all the samples were studied using thermogravimetric analysis (TGA) and Broido’s method was employed to analyse the kinetic parameters involved in different degradation steps. All the composite films were sandwitched between disk shape gold electrodes; electrical contacts were established to study the dielectric properties. The influence of Pd loading on the dielectric properties of PVC were examined. Finally, the catalytic properties of Pd0 composites were studied using standard model reduction reaction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of aqueous sodium borohydride and reported.
DOI: 10.6084/m9.figshare.8848286
title: Synthesis and characterization of amphiphilic block copolymers by transesterification for nanoparticle production

description: Abstract Poly(ε-caprolactone)-block-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, triblock) and Poly(ε-caprolactone)-block-(poly(ethylene oxide)−poly(propylene oxide)−poly(ethylene oxide)-poly (ε-caprolactone) (PCL-PEO-PPO-PEO-PCL, pentablock) copolymers were synthesized by transesterification with reduction of PCL molecular mass, enabling fewer reactions, lower temperatures, and eliminating extensive purification steps. Free hydrophilic groups were removed from the samples by selective precipitation, and 1H-NMR, FTIR, GPC and DSC analyses characterized the structure and properties of the resulting copolymers. The detection of remaining hydrophilic groups indicates the formation of the amphiphilic block copolymers (BCPs). Further, we obtained polymeric nanoparticles with monodisperse size distribution profiles by nano-precipitation from both the triblock and the pentablock copolymers using a microfluidic device, resulting 144.6 and 188.9 nm size and 0.093 and 0.102 nm polydispersity index, respectively. The nanoparticle assembly depends on the copolymer composition, and the possibility of nanoparticle assembly corroborates to the block structure of the copolymers, and the success of this synthesis route to obtain BCPs.
DOI: 10.6084/m9.figshare.8848280
title: Fatty Acid Determination in Fermented Milk Samples Employing Direct Esterification and Gas Chromatography

description: The developed method (DM) allows to perform experiments in shorter time with reduced amount of sample and reagent for fatty acids (FA) determination in fermented milk samples. Design Expert software was employed to discover the greatest sonication time (7 and 20 min) and concentration for alkaline and acidic reactions (0.70 and 1.5 mol L-1). Validation procedures were applied in yogurt samples with diverse fat content, ranging from 1.22 to 2.83%, being in agreement with acceptable values. Besides, the intraday relative standard deviation (RSD) (1.86%) and interday RSD (4.23%) values confirmed the good accuracy of the proposed method for FA derivatization.
DOI: 10.6084/m9.figshare.8848283.v1
title: Thermal, dielectric and catalytic behavior of palladium doped PVC films

description: Abstract The present paper discusses the aspects of synthesizing palladium (Pd) doped (Pd+2 and Pd0) poly(vinyl chloride) (PVC) using simple solution cast technique. The Pd loading to PVC was altered from 2.5% to 10.0% and the material properties were studied using UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDX) and Field Emission Scanning Electron Microscopy (FE-SEM). Thermal behavior of all the samples were studied using thermogravimetric analysis (TGA) and Broido’s method was employed to analyse the kinetic parameters involved in different degradation steps. All the composite films were sandwitched between disk shape gold electrodes; electrical contacts were established to study the dielectric properties. The influence of Pd loading on the dielectric properties of PVC were examined. Finally, the catalytic properties of Pd0 composites were studied using standard model reduction reaction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of aqueous sodium borohydride and reported.
DOI: 10.6084/m9.figshare.8848277
title: Maturation and Maceration Effects on Tropical Red Wines Assessed by Chromatography and Analysis of Variance - Principal Component Analysis

description: Effects of grape maturity, maceration length during winemaking and interaction of both of them on physicochemical parameters, phenolic and volatile composition were investigated in tropical Syrah wines with the aim of finding the best conditions to produce wines that are rich in phenols and positive aroma active compounds. A headspace solid phase micro extraction and gas chromatography with mass spectrometry (HS-SPME-GC-MS) method was improved and validated for the analysis of 41 volatiles. Principal component analysis (PCA) and analysis of variance (ANOVA)-PCA coupled with inspection of statistically significant loadings were important to access the effects of maturation and maceration on wine composition. Wines made from riper grapes (21-23 ºBrix) stood out in relation to color intensity, total phenolic compounds, and presented high levels of anthocyanins, flavonols, procyanidin A2, and linalool (floral). Thirty days of maceration were linked to higher levels of some flavan-3-ols, gallic acid, a few esters (fruity) and alcohols, (E)-nerolidol (floral), while nonanoic acid (unpleasant aroma) reduced its concentration with prolonged maceration.
DOI: 10.6084/m9.figshare.8848280.v1
title: Fatty Acid Determination in Fermented Milk Samples Employing Direct Esterification and Gas Chromatography

description: The developed method (DM) allows to perform experiments in shorter time with reduced amount of sample and reagent for fatty acids (FA) determination in fermented milk samples. Design Expert software was employed to discover the greatest sonication time (7 and 20 min) and concentration for alkaline and acidic reactions (0.70 and 1.5 mol L-1). Validation procedures were applied in yogurt samples with diverse fat content, ranging from 1.22 to 2.83%, being in agreement with acceptable values. Besides, the intraday relative standard deviation (RSD) (1.86%) and interday RSD (4.23%) values confirmed the good accuracy of the proposed method for FA derivatization.
DOI: 10.6084/m9.figshare.8848286.v1
title: Synthesis and characterization of amphiphilic block copolymers by transesterification for nanoparticle production

description: Abstract Poly(ε-caprolactone)-block-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, triblock) and Poly(ε-caprolactone)-block-(poly(ethylene oxide)−poly(propylene oxide)−poly(ethylene oxide)-poly (ε-caprolactone) (PCL-PEO-PPO-PEO-PCL, pentablock) copolymers were synthesized by transesterification with reduction of PCL molecular mass, enabling fewer reactions, lower temperatures, and eliminating extensive purification steps. Free hydrophilic groups were removed from the samples by selective precipitation, and 1H-NMR, FTIR, GPC and DSC analyses characterized the structure and properties of the resulting copolymers. The detection of remaining hydrophilic groups indicates the formation of the amphiphilic block copolymers (BCPs). Further, we obtained polymeric nanoparticles with monodisperse size distribution profiles by nano-precipitation from both the triblock and the pentablock copolymers using a microfluidic device, resulting 144.6 and 188.9 nm size and 0.093 and 0.102 nm polydispersity index, respectively. The nanoparticle assembly depends on the copolymer composition, and the possibility of nanoparticle assembly corroborates to the block structure of the copolymers, and the success of this synthesis route to obtain BCPs.
DOI: 10.6084/m9.figshare.8848268
title: Ingestive behavior of dairy goats fed diets containing increasing levels of neutral detergent fiber and particle size using multivariate analysis

description: ABSTRACT. The purpose of this study was to evaluate the effect of interaction between increasing neutral detergent fiber content and particle sizes on ingestive behavior of dairy goats. Forty-eight lactating, multiparous Saanen and Alpine goats, with average milk production of 1.4 ±0.57 kg d-1, around 60th ±12 day of lactation were distributed in a 3 x 4 factorial completely randomized design. The diets consisted: three particles sizes (02, 05 or 15 cm) and four levels of neutral detergent fiber (34, 41, 49 or 57% NDFf) from forage (Tifton 85 hay). The ingestive behavior was monitored during 24 hours. A regression and a multivariate time series cluster analysis were performed. No interaction was found (p &gt; 0.05) between treatments. Feeding time was different according to the particle size, having an increasing linear effect. Rumination and idle times were not affected (p &gt; 0.05). The temporal feeding behavior was clustered into two groups according to the profile of particle size of the diet. Rumination peaks were randomly distributed with more intense activity before morning and afternoon meals. The increase in NDFf content in the diet did not change the ingestive behavior. The multivariate cluster analysis in a time series data is useful to interpret animal feeding behavior.
DOI: 10.6084/m9.figshare.8848271
title: Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro

description: ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the TUNEL Test. The use of antioxidants during cultivation did not increase (P&gt; 0.05) the final blastocyst production rate. The treatments T2, T3, T4 and T5 had the lowest (P&lt; 0.05) apoptotic indexes (4.5±1.1%, 8.4±2.5%, 3.4±1.1% and 5.5±0.9%, respectively) when compared to T1 treatment (10.0±1.4%). The number of inner cell mass did not differ (P&gt; 0.05) among embryos from different treatments. The addition of antioxidant obtained from oily extract of Lippia origanoides reduces the apoptosis rate and improves the quality without increasing the total in vitro production of bovine embryos.
DOI: 10.6084/m9.figshare.8848274
title: Study on mechanical & thermal properties of PCL blended graphene biocomposites

description: Abstract Graphene is a new carbon based nonmaterial that attracts the technology and constitutes one of the great promises for nanotechnology applications in a near feature. It’s having versatile intrinsic mechanical, thermal and electrical properties.By Incorporation of small amount of graphene fillers into polymer matrix can create attractive bio composites with different morphological and functional properties. The development of biomaterials with special properties is a requirement in biomedical research, particularly in biomedical application. The aim of this work was to develop biocompatible, usable bio composites for biomedical applications using graphene as filler. Recent research evidenced that grapheme-polymer bio composites are promising materials with applications ranging from transportation, biomedical systems, sensors, electrodes for solar panels and EMI.Chemically converted graphene (CCG) solution were prepared through reduction of GO, and Polycaprolactone (PCl), a synthetic biodegradable and biocompatible aliphatic polyester also a suitable for developing biocomposites.
DOI: 10.6084/m9.figshare.8848268.v1
title: Ingestive behavior of dairy goats fed diets containing increasing levels of neutral detergent fiber and particle size using multivariate analysis

description: ABSTRACT. The purpose of this study was to evaluate the effect of interaction between increasing neutral detergent fiber content and particle sizes on ingestive behavior of dairy goats. Forty-eight lactating, multiparous Saanen and Alpine goats, with average milk production of 1.4 ±0.57 kg d-1, around 60th ±12 day of lactation were distributed in a 3 x 4 factorial completely randomized design. The diets consisted: three particles sizes (02, 05 or 15 cm) and four levels of neutral detergent fiber (34, 41, 49 or 57% NDFf) from forage (Tifton 85 hay). The ingestive behavior was monitored during 24 hours. A regression and a multivariate time series cluster analysis were performed. No interaction was found (p &gt; 0.05) between treatments. Feeding time was different according to the particle size, having an increasing linear effect. Rumination and idle times were not affected (p &gt; 0.05). The temporal feeding behavior was clustered into two groups according to the profile of particle size of the diet. Rumination peaks were randomly distributed with more intense activity before morning and afternoon meals. The increase in NDFf content in the diet did not change the ingestive behavior. The multivariate cluster analysis in a time series data is useful to interpret animal feeding behavior.
DOI: 10.6084/m9.figshare.8848271.v1
title: Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro

description: ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the TUNEL Test. The use of antioxidants during cultivation did not increase (P&gt; 0.05) the final blastocyst production rate. The treatments T2, T3, T4 and T5 had the lowest (P&lt; 0.05) apoptotic indexes (4.5±1.1%, 8.4±2.5%, 3.4±1.1% and 5.5±0.9%, respectively) when compared to T1 treatment (10.0±1.4%). The number of inner cell mass did not differ (P&gt; 0.05) among embryos from different treatments. The addition of antioxidant obtained from oily extract of Lippia origanoides reduces the apoptosis rate and improves the quality without increasing the total in vitro production of bovine embryos.
DOI: 10.6084/m9.figshare.8848277.v1
title: Maturation and Maceration Effects on Tropical Red Wines Assessed by Chromatography and Analysis of Variance - Principal Component Analysis

description: Effects of grape maturity, maceration length during winemaking and interaction of both of them on physicochemical parameters, phenolic and volatile composition were investigated in tropical Syrah wines with the aim of finding the best conditions to produce wines that are rich in phenols and positive aroma active compounds. A headspace solid phase micro extraction and gas chromatography with mass spectrometry (HS-SPME-GC-MS) method was improved and validated for the analysis of 41 volatiles. Principal component analysis (PCA) and analysis of variance (ANOVA)-PCA coupled with inspection of statistically significant loadings were important to access the effects of maturation and maceration on wine composition. Wines made from riper grapes (21-23 ºBrix) stood out in relation to color intensity, total phenolic compounds, and presented high levels of anthocyanins, flavonols, procyanidin A2, and linalool (floral). Thirty days of maceration were linked to higher levels of some flavan-3-ols, gallic acid, a few esters (fruity) and alcohols, (E)-nerolidol (floral), while nonanoic acid (unpleasant aroma) reduced its concentration with prolonged maceration.