cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12340724.v4
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i> <sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i>
 <sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i>
 <sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i>
 <sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i>
 <sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.12340724.v9
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i><sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i><sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i><sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i><sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i><sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i><sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i><sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i><sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i><sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.14437583.v1
title: Additional file 2 of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

description: Additional file 2: Specific genes, shared genes and disrupted open reading frames. Contains lists of isolate specific genes, genes shared between two isolates and putative pseudogenes. A list of artificially disrupted open reading frames in the P15 data is also included. (XLS 264 KB)
DOI: 10.6084/m9.figshare.14437589
title: Additional file 4 of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

description: Additional file 4: Conservation of meiosis, DNA repair and ultraconserved proteins. Conservation of putative Meiosis and DNA repair proteins in WB, GS and P15. Protein identities are recorded as percent extracted from ClustalW alignments of orthologs in WB, GS and P15. Several major deletions or insertions that are lineage specific were recorded. Most of these were specific for the GS lineage. A list of ultraconserved proteins in the genomes is included. (XLS 21 KB)
DOI: 10.6084/m9.figshare.20729314.v1
title: ANALYSIS AND INTERVENTIONS OF COMMON SPORTS INJURIES IN COLLEGE BASKETBALL PLAYERS

description: ABSTRACT Introduction As college basketball continues to develop, more and more college students are joining the sport, and the resulting sports injuries have also gradually increased, affecting the quality of life and academic performance. Objective Analyze the most common sports injuries and offer solutions for the safe practice of college basketball. Methods This article studies, through interviews and literature review, the basketball injuries of college students, analyze the main causes of sports injuries, and presents corresponding countermeasures to promote the safe development of sports education in colleges. Results The main injuries were during training, excessive force, and inadequate preparation. Deficient muscle work is also associated with most of these injuries. Conclusion Basketball is highly competitive and popular on college campuses. It is a sport that can exercise and enrich the extracurricular life of college students, requiring from its practitioners greater attention in the pre-training warm-up phase and parallel muscle strengthening to reduce its major injuries in college students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes
DOI: 10.6084/m9.figshare.12340724.v17
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i> <sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i>
 <sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i>
 <sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i>
 <sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i>
 <sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.12864937
title: Additional file 9 of Upregulation of METTL14 mediates the elevation of PERP mRNA N6 adenosine methylation promoting the growth and metastasis of pancreatic cancer

description: Additional file 9: Table S4. shMETTL14 m6A experiment peak annotation.
DOI: 10.6084/m9.figshare.14437586
title: Additional file 3 of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

description: Additional file 3: List of Giardia non-core and core genes. A list of non-core genes in the P15 genome and Giardia core genes. A few genes lack annotated orthologs due to an incomplete genome assembly. (XLS 672 KB)
DOI: 10.6084/m9.figshare.14437589.v1
title: Additional file 4 of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

description: Additional file 4: Conservation of meiosis, DNA repair and ultraconserved proteins. Conservation of putative Meiosis and DNA repair proteins in WB, GS and P15. Protein identities are recorded as percent extracted from ClustalW alignments of orthologs in WB, GS and P15. Several major deletions or insertions that are lineage specific were recorded. Most of these were specific for the GS lineage. A list of ultraconserved proteins in the genomes is included. (XLS 21 KB)
DOI: 10.6084/m9.figshare.12340724.v2
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i> <sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i>
 <sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i>
 <sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i>
 <sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i>
 <sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.12864934.v1
title: Additional file 8 of Upregulation of METTL14 mediates the elevation of PERP mRNA N6 adenosine methylation promoting the growth and metastasis of pancreatic cancer

description: Additional file 8: Table S3. shMETTL14 vs shCtrl differential expression.
DOI: 10.6084/m9.figshare.12864956.v1
title: Additional file 2 of Predicting viral exposure response from modeling the changes of co-expression networks using time series gene expression data

description: Additional file 2 An example of the R code used in the paper.
DOI: 10.6084/m9.figshare.14437559.v9
title: Additional file of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

description: Additional file of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate
DOI: 10.6084/m9.figshare.20729295
title: THE EFFECT OF DISTANCE LEARNING IN COLLEGES AND UNIVERSITIES ON PHYSICAL HEALTH PROMOTION IN COLLEGE STUDENTS

description: ABSTRACT Introduction Influenced by the traditional teaching methods of physical education, the teaching activities of athletics are exclusive to the outdoor environment, which is very common in China, resulting in many disadvantages in teaching these sports by distance, restricting career development and requiring adaptations due to current circumstances. Objective Provide a theoretical basis to further improve the level of physical health of university students and substantiate the necessary adaptations to the teaching of athletics in colleges and universities. Methods This paper reviews the physical health status of university students, raises the reasons for their decline, and analyzes the role of teaching athletics activities in promoting the physical health of university students. Results The performance of physical activities related to athletics promotes the physical health of university students. Conclusion Athletic sports play an important role in the health promotion of university students, not receiving proper care currently, lacking more attention in its promotion by responsible agents. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
DOI: 10.6084/m9.figshare.12340724.v1
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i><sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i><sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i><sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i><sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i><sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i><sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i><sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i><sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i><sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.12340724.v11
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i><sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i><sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i><sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i><sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i><sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i><sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i><sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i><sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i><sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.12340724.v12
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i> <sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i>
 <sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i>
 <sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i>
 <sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i>
 <sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.12340724.v14
title: The type I-E CRISPR-Cas system influences the acquisition of <i>bla</i> <sub>KPC</sub>-IncF plasmid in <i>Klebsiella pneumonia</i>

description: <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>K. pneumoniae</i> (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids are favored by CG258 <i>K. pneumoniae,</i> it was of interest to examine the co-distribution of CRISPR and <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in such isolates. We collected 459 clinical <i>K. pneumoniae isolates</i> in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and <i>bla</i>
 <sub>KPC</sub>-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in <i>K. pneumoniae</i> could effectively hindered <i>bla</i>
 <sub>KPC</sub>-IncF plasmids invasion and existence. Notably, most <i>bla</i>
 <sub>KPC</sub>-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of <i>bla</i>
 <sub>KPC</sub> in <i>K. pneumoniae</i> populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of <i>bla</i>
 <sub>KPC</sub>-IncF plasmids in CG258 <i>K. pneumoniae</i>.
DOI: 10.6084/m9.figshare.14437586.v1
title: Additional file 3 of Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

description: Additional file 3: List of Giardia non-core and core genes. A list of non-core genes in the P15 genome and Giardia core genes. A few genes lack annotated orthologs due to an incomplete genome assembly. (XLS 672 KB)
DOI: 10.6084/m9.figshare.20729334
title: EXPERIMENTAL STUDY OF EXERCISE PRESCRIPTION INTERVENTION ON THE UNIVERSITY STUDENTS’ HEALTH

description: ABSTRACT Introduction Disparate to the development of society, health indices in Chinese college students have shown a pronounced decline in recent years. Government concern over this recent challenge has encouraged research for practical solutions, including optimized physical activity protocols. Objective Explore the effects of an exercise intervention on the health of Chinese university students. Methods Full-time university students (80 males and 55 females) were selected and randomly assigned into two groups. Before and after the experiment, the college students’ body composition scale and exercise experience were tested, focusing on the relationship between exercise prescription and changes in physical and mental health indicators. Results The body fat percentage of male and female students decreased significantly after the experiment. After eight weeks of the exercise prescription experiment, the weight of male and female college students was controlled or reduced. The suggested exercise prescription achieved a good moderating effect on the weight of the volunteers. There was a significant difference between the two groups, indicating that the experiment had a beneficial effect on vital capacity (p&lt;0.01). Conclusion The suggested exercise prescription proved to be feasible to guide and intervene in the physical exercise of college students aiming at a beneficial impact on the physical health of college students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.