cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/mrdpkb5jrd
title: Single-nucleus RNA-seq of archived PAXgene whole blood samples

description: Summary - No method has been developed for single-cell analysis of the large repositories of preserved whole blood samples stored in PAXgene Blood RNA tubes. To address this gap, two nuclei isolation techniques for single-nucleus RNA sequencing were evaluated: mechanical separation (MS), using an Acrodisc filter, and cell lysis (CL). While both methods captured nuclei from all major immune cell types, CL resulted in up to two orders of magnitude higher nuclei yields and less biased proportions of immune cells than MS. High ambient globin gene counts following CL were substantially reduced by CRISPR-guided globin gene depletion of complementary DNA, resulting in more sensitive and efficient gene detection per cell. Despite capturing only nuclear transcripts, CL-derived samples maintained similar cell-type proportions and gene expression as matched peripheral blood mononuclear cell samples, while retaining granulocytes. The CL isolation with globin depletion method enables comprehensive analysis of PAXgene whole blood samples at single-cell resolution.
Experimental design - Three healthy donor blood was used to extract PBMC and prepare PAXgene blood RNA tubes. Frozen PAXgene tubes were later slowly thawed to perform MS and CL methods. MS focused on separating white blood cells by Acrodisc filter whereas CL focused on lysing of the blood pellet from PAXgene blood tubes. Matched PBMCs were also processed. All using 10x genomics v3.1 3’ gene expression assay. 
Extract protocol - Human donor blood A, B and C were commercially obtained from research blood components (https://www.researchbloodcomponents.com/). PBMCs were extracted and additional blood was stored in PAXgene blood RNA tubes. PBMCs were stored for long term in liquid nitrogen. PAXgene blood RNA tubes were stored at room temperature for 2 hours and then stored in minus 80⁰C. PBMCs were processed for scRNA-seq and PAXgene blood RNA tubes for snRNA-seq using 10X genomics v3.1 3' gene expression assay for single cell sequencing. Single cell suspension from PBMC was prepared after slowing thawing frozen PBMCs followed by washing in RPMI 0.1% BSA. Nuclei suspension was prepared from PAXgene blood RNA tubes for mechanical separation and Cell lysis as detailed in supplementary of preprint https://doi.org/10.1101/2025.03.06.641241 and in depth Cell lysis with depletion in protocols.io link. https://dx.doi.org/10.17504/protocols.io.n92ld6yxxg5b/v1. 10X genomics library construction protocol CG000315 Rev E was used for preparation of all libraries. CRIPSR Globin depletion-protcols.io https://dx.doi.org/10.17504/protocols.io.n92ld6yxxg5b/v1
Data processing step - The cellranger mkfastq pipeline (version 6.1.1; 10x Genomics) was used to demultiplex the base call files (BCLs) to FASTQ files, and then the cellranger count pipeline was used for downstream processing steps, including alignment to reference genome GRCh38, counting of unique molecular identifiers (UMIs), and cell calling. 
SRA - PRJNA1379454
DOI: 10.17632/mrdpkb5jrd.2
title: Single-nucleus RNA-seq of archived PAXgene whole blood samples

description: Summary - No method has been developed for single-cell analysis of the large repositories of preserved whole blood samples stored in PAXgene Blood RNA tubes. To address this gap, two nuclei isolation techniques for single-nucleus RNA sequencing were evaluated: mechanical separation (MS), using an Acrodisc filter, and cell lysis (CL). While both methods captured nuclei from all major immune cell types, CL resulted in up to two orders of magnitude higher nuclei yields and less biased proportions of immune cells than MS. High ambient globin gene counts following CL were substantially reduced by CRISPR-guided globin gene depletion of complementary DNA, resulting in more sensitive and efficient gene detection per cell. Despite capturing only nuclear transcripts, CL-derived samples maintained similar cell-type proportions and gene expression as matched peripheral blood mononuclear cell samples, while retaining granulocytes. The CL isolation with globin depletion method enables comprehensive analysis of PAXgene whole blood samples at single-cell resolution.
Experimental design - Three healthy donor blood was used to extract PBMC and prepare PAXgene blood RNA tubes. Frozen PAXgene tubes were later slowly thawed to perform MS and CL methods. MS focused on separating white blood cells by Acrodisc filter whereas CL focused on lysing of the blood pellet from PAXgene blood tubes. Matched PBMCs were also processed. All using 10x genomics v3.1 3’ gene expression assay. 
Extract protocol - Human donor blood A, B and C were commercially obtained from research blood components (https://www.researchbloodcomponents.com/). PBMCs were extracted and additional blood was stored in PAXgene blood RNA tubes. PBMCs were stored for long term in liquid nitrogen. PAXgene blood RNA tubes were stored at room temperature for 2 hours and then stored in minus 80⁰C. PBMCs were processed for scRNA-seq and PAXgene blood RNA tubes for snRNA-seq using 10X genomics v3.1 3' gene expression assay for single cell sequencing. Single cell suspension from PBMC was prepared after slowing thawing frozen PBMCs followed by washing in RPMI 0.1% BSA. Nuclei suspension was prepared from PAXgene blood RNA tubes for mechanical separation and Cell lysis as detailed in supplementary of preprint https://doi.org/10.1101/2025.03.06.641241 and in depth Cell lysis with depletion in protocols.io link. https://dx.doi.org/10.17504/protocols.io.n92ld6yxxg5b/v1. 10X genomics library construction protocol CG000315 Rev E was used for preparation of all libraries. CRIPSR Globin depletion-protcols.io https://dx.doi.org/10.17504/protocols.io.n92ld6yxxg5b/v1
Data processing step - The cellranger mkfastq pipeline (version 6.1.1; 10x Genomics) was used to demultiplex the base call files (BCLs) to FASTQ files, and then the cellranger count pipeline was used for downstream processing steps, including alignment to reference genome GRCh38, counting of unique molecular identifiers (UMIs), and cell calling. 
SRA - PRJNA1379454
DOI: 10.17632/krbvmj2pss
title: Assessing the Clinical Significance of Cervical Cultures in Postpartum Endometritis: Focus on Enterobacterales

description: Cervical cultures - focus on enterobacterials data tables
DOI: 10.17632/krbvmj2pss.1
title: Assessing the Clinical Significance of Cervical Cultures in Postpartum Endometritis: Focus on Enterobacterales

description: Cervical cultures - focus on enterobacterials data tables
DOI: 10.17632/dzrgwdxhgp
title: Apremilast Improves Skin Outcomes in Pediatric Plaque Psoriasis of Shorter Disease Duration: 52-Week Results From the SPROUT Phase 3 Trial

description: Supplemental data for an article published in the Journal of the American Academy of Dermatology.
DOI: 10.17632/dzrgwdxhgp.1
title: Apremilast Improves Skin Outcomes in Pediatric Plaque Psoriasis of Shorter Disease Duration: 52-Week Results From the SPROUT Phase 3 Trial

description: Supplemental data for an article published in the Journal of the American Academy of Dermatology.
DOI: 10.17632/2zhrd6by36
title: Geothermal Power Plants

description: The dataset comprises land price and various location-specific characteristics, which are used to analyze the potential impact of geothermal power plants on land values.
DOI: 10.17632/2zhrd6by36.2
title: Geothermal Power Plants

description: The dataset comprises land price and various location-specific characteristics, which are used to analyze the potential impact of geothermal power plants on land values.
DOI: 10.60797/irj.2026.163.87.1
title: Показатели продуктивности телок в первую лактацию, с учетом линейной принадлежности (n=418)

description: Международный научно-исследовательский журнал, Выпуск 1 (163) 2026
DOI: 10.60797/irj.2026.163.87.2
title: Продуктивные качества коров во вторую лактацию, с учетом линейной принадлежности (n=345)

description: Международный научно-исследовательский журнал, Выпуск 1 (163) 2026
DOI: 10.60797/irj.2026.163.87.3
title: Продуктивность коров различных линий за третью лактацию (n=197)

description: Международный научно-исследовательский журнал, Выпуск 1 (163) 2026
DOI: 10.6084/m9.figshare.30870640
title: Efficacy, safety, and patient-centered outcomes of needle-free injectors versus insulin pens in diabetes management: a comprehensive meta-analysis

description: To evaluate the comparative effectiveness, safety, and patient acceptance of needle-free injectors and insulin pens through a meta-analysis. A thorough literature search was performed across multiple databases, including CNKI, the Weipu Database, the Wanfang Database, PubMed, Embase, and The Cochrane Library for randomized controlled trials (RCTs) on the use of needle-free injectors and insulin pens in diabetes treatment, with a time frame from database inception until June 2024. Five researchers carefully screened the literature in accordance with the inclusion and exclusion criteria, assessed study quality with validated tools and conducted the meta-analysis using RevMan 5.4. This study included 22 publications encompassing 22 studies, involving a total of 2,580 patients, with 1,290 in the needle-free group and 1,290 in the insulin pen group. The findings suggest that needle-free injectors were superior to insulin pens in improving treatment efficacy, local adverse reactions, and increasing patient acceptability, with statistically significant differences. However, no significant variations were observed in continuous glucose monitoring. Needle-free injectors can improve certain aspects of glycemic control, such as reducing HbA1c, FPG, and 2hPPG; improve local adverse reactions and increase patient acceptability. However, improvements in glycemic variability metrics were not statistically significant, indicating that further research is needed to fully assess their long-term safety and therapeutic effectiveness. In terms of systemic safety, the risk of hypoglycemia in the needle-free group was not significantly different from that in the needle group (<i>P</i> = 0.14). Additionally, our publication bias analysis revealed potential biases in the outcomes related to hypoglycemia and insulin dosage.
DOI: 10.6084/m9.figshare.30870640.v1
title: Efficacy, safety, and patient-centered outcomes of needle-free injectors versus insulin pens in diabetes management: a comprehensive meta-analysis

description: To evaluate the comparative effectiveness, safety, and patient acceptance of needle-free injectors and insulin pens through a meta-analysis. A thorough literature search was performed across multiple databases, including CNKI, the Weipu Database, the Wanfang Database, PubMed, Embase, and The Cochrane Library for randomized controlled trials (RCTs) on the use of needle-free injectors and insulin pens in diabetes treatment, with a time frame from database inception until June 2024. Five researchers carefully screened the literature in accordance with the inclusion and exclusion criteria, assessed study quality with validated tools and conducted the meta-analysis using RevMan 5.4. This study included 22 publications encompassing 22 studies, involving a total of 2,580 patients, with 1,290 in the needle-free group and 1,290 in the insulin pen group. The findings suggest that needle-free injectors were superior to insulin pens in improving treatment efficacy, local adverse reactions, and increasing patient acceptability, with statistically significant differences. However, no significant variations were observed in continuous glucose monitoring. Needle-free injectors can improve certain aspects of glycemic control, such as reducing HbA1c, FPG, and 2hPPG; improve local adverse reactions and increase patient acceptability. However, improvements in glycemic variability metrics were not statistically significant, indicating that further research is needed to fully assess their long-term safety and therapeutic effectiveness. In terms of systemic safety, the risk of hypoglycemia in the needle-free group was not significantly different from that in the needle group (<i>P</i> = 0.14). Additionally, our publication bias analysis revealed potential biases in the outcomes related to hypoglycemia and insulin dosage.
DOI: 10.6084/m9.figshare.28182291
title: In vivo antitumor activity of PHT-427 inhibitor-loaded polymeric nanoparticles in head and neck squamous cell carcinoma

description: Recent studies on head and neck squamous cell carcinoma (HNSCC) tumorigenesis have revealed several dysregulated molecular pathways. The phosphatidylinositol-3-kinase (PI3K) signaling pathway is frequently activated in HNSCC, making it an attractive target for therapies. PHT-427 is a dual inhibitor of PI3K and the mammalian target of AKT/PDK1. This study evaluates the anticancer efficacy of the inhibitor PHT-427 loaded into polymeric nanoparticles (NP) based on α-TOS (NP-427) administered by intratumoral injection into a hypopharyngeal squamous cell carcinoma (FaDu cells) heterotopic xenograft mouse model. The nanocarrier system, based on block copolymers of N-vinylpyrrolidone (VP) and a methacrylic derivative of α-TOS (MTOS), was synthesized, and PHT-427 was loaded into the delivery system. First, we evaluated the effect of NP-427 on tumor growth by measuring tumor volume, mouse weight, survival, and the development of tumor ulceration and necrosis. In addition, we measured PI3KCA/AKT/PDK1 gene expression, PI3KCA/AKT/PDK1 protein levels, Epidermal Growth Factor Receptor (EGFR), and angiogenesis in the tumor tissue. PHT-427 encapsulation increased drug efficacy and safety, as demonstrated by decreased tumor volume, reduced PI3K/AKT/PDK1 pathway expression, and improved antitumor activity and necrosis induction in the mouse xenograft model. EGFR and angiogenesis marker (Factor VIII) expression were significantly lower in the NP-427 group compared to other experimental groups. Administration of encapsulated PHT-427 at the tumor sites proves promising for HNSCC therapy.
DOI: 10.6084/m9.figshare.28182291.v1
title: In vivo antitumor activity of PHT-427 inhibitor-loaded polymeric nanoparticles in head and neck squamous cell carcinoma

description: Recent studies on head and neck squamous cell carcinoma (HNSCC) tumorigenesis have revealed several dysregulated molecular pathways. The phosphatidylinositol-3-kinase (PI3K) signaling pathway is frequently activated in HNSCC, making it an attractive target for therapies. PHT-427 is a dual inhibitor of PI3K and the mammalian target of AKT/PDK1. This study evaluates the anticancer efficacy of the inhibitor PHT-427 loaded into polymeric nanoparticles (NP) based on α-TOS (NP-427) administered by intratumoral injection into a hypopharyngeal squamous cell carcinoma (FaDu cells) heterotopic xenograft mouse model. The nanocarrier system, based on block copolymers of N-vinylpyrrolidone (VP) and a methacrylic derivative of α-TOS (MTOS), was synthesized, and PHT-427 was loaded into the delivery system. First, we evaluated the effect of NP-427 on tumor growth by measuring tumor volume, mouse weight, survival, and the development of tumor ulceration and necrosis. In addition, we measured PI3KCA/AKT/PDK1 gene expression, PI3KCA/AKT/PDK1 protein levels, Epidermal Growth Factor Receptor (EGFR), and angiogenesis in the tumor tissue. PHT-427 encapsulation increased drug efficacy and safety, as demonstrated by decreased tumor volume, reduced PI3K/AKT/PDK1 pathway expression, and improved antitumor activity and necrosis induction in the mouse xenograft model. EGFR and angiogenesis marker (Factor VIII) expression were significantly lower in the NP-427 group compared to other experimental groups. Administration of encapsulated PHT-427 at the tumor sites proves promising for HNSCC therapy.
DOI: 10.6084/m9.figshare.29222895
title: Novel ethosomal gel formulation for enhanced transdermal delivery of curcumin and cyclosporine: a preclinical approach to rheumatoid arthritis management

description: Vesicular systems have demonstrated efficacy in the management of Rheumatoid Arthritis (RA). This study explores the synergistic effect of edge-activated ethosomal gel to enhance the transdermal delivery of Curcumin (CUR) and Cyclosporine (CYC). Ethosomal vesicles prepared via the ethanol injection method were incorporated into a gel, with the optimized formulation exhibiting an average particle size of 93.3 ± 1.17 nm and a zeta potential of −29.2 ± 0.17 mV. <i>Ex vivo</i> diffusion studies on porcine ear skin demonstrated 97.115 ± 0.40% CUR and 98.331 ± 1.08% CYC release over 18 hours, exhibiting Hixson-Crowell diffusion mechanisms. The steady-state flux and permeability coefficients were 0.095 µg/cm<sup>2</sup>/hr and 0.0095 cm/hr for CUR, and 0.0804 µg/cm<sup>2</sup>/hr and 0.01608 cm/hr for CYC respectively. In anti-inflammatory tests on lipopolysaccharide (LPS)-induced RAW 264.7 cells, the gel significantly increased IL-10 levels (p &lt; 0.001), inhibited prostaglandin-E2, and reduced IL-6 and TNF-α levels (p &lt; 0.001). Moreover, the ethosomal gel demonstrated nonirritating properties and exhibited significant reduction in arthritic symptoms in the Complete Freund’s Adjuvant induced 28-day rat model, surpassing the effects of marketed and conventional gel. These findings highlight the synergistic benefits of combining CUR and CYC in an ethosomal gel, offering a promising alternative for RA management. Future clinical investigations are warranted to validate its safety and efficacy in humans and facilitate potential therapeutic integration.
DOI: 10.6084/m9.figshare.29222895.v1
title: Novel ethosomal gel formulation for enhanced transdermal delivery of curcumin and cyclosporine: a preclinical approach to rheumatoid arthritis management

description: Vesicular systems have demonstrated efficacy in the management of Rheumatoid Arthritis (RA). This study explores the synergistic effect of edge-activated ethosomal gel to enhance the transdermal delivery of Curcumin (CUR) and Cyclosporine (CYC). Ethosomal vesicles prepared via the ethanol injection method were incorporated into a gel, with the optimized formulation exhibiting an average particle size of 93.3 ± 1.17 nm and a zeta potential of −29.2 ± 0.17 mV. <i>Ex vivo</i> diffusion studies on porcine ear skin demonstrated 97.115 ± 0.40% CUR and 98.331 ± 1.08% CYC release over 18 hours, exhibiting Hixson-Crowell diffusion mechanisms. The steady-state flux and permeability coefficients were 0.095 µg/cm<sup>2</sup>/hr and 0.0095 cm/hr for CUR, and 0.0804 µg/cm<sup>2</sup>/hr and 0.01608 cm/hr for CYC respectively. In anti-inflammatory tests on lipopolysaccharide (LPS)-induced RAW 264.7 cells, the gel significantly increased IL-10 levels (p &lt; 0.001), inhibited prostaglandin-E2, and reduced IL-6 and TNF-α levels (p &lt; 0.001). Moreover, the ethosomal gel demonstrated nonirritating properties and exhibited significant reduction in arthritic symptoms in the Complete Freund’s Adjuvant induced 28-day rat model, surpassing the effects of marketed and conventional gel. These findings highlight the synergistic benefits of combining CUR and CYC in an ethosomal gel, offering a promising alternative for RA management. Future clinical investigations are warranted to validate its safety and efficacy in humans and facilitate potential therapeutic integration.
DOI: 10.17632/r7yyj8yfhb
title: Supplemental files for AD Brief reports

description: Probe-Guided Core Excision for Complete Sinus Tract Removal in Pilonidal Disease
DOI: 10.17632/r7yyj8yfhb.1
title: Supplemental files for AD Brief reports

description: Probe-Guided Core Excision for Complete Sinus Tract Removal in Pilonidal Disease
DOI: 10.17632/6p865gztg6
title: Industrial Sulfur Gas Concentration Dataset for SRU Forecasting

description: This dataset contains raw industrial H2S/SO2 concentration sequences and processed subsequences (via VMD-CEEMDAN) used in the study "Hybrid Multiscale Forecasting of SRU Sulfur Gas Concentrations Using Optimized PatchTST". It is provided to support the reproducibility of the forecasting results and performance metrics reported in the paper.
DOI: 10.17632/6p865gztg6.1
title: Industrial Sulfur Gas Concentration Dataset for SRU Forecasting

description: This dataset contains raw industrial H2S/SO2 concentration sequences and processed subsequences (via VMD-CEEMDAN) used in the study "Hybrid Multiscale Forecasting of SRU Sulfur Gas Concentrations Using Optimized PatchTST". It is provided to support the reproducibility of the forecasting results and performance metrics reported in the paper.
DOI: 10.17632/6jmyj9g6rj
title: From Digital Infrastructure to Breakthrough Innovation: Evidence from China

description: README: Replication Package 
Paper Title: From Digital Infrastructure to Breakthrough Innovation: Evidence from China

Authors:
Peiyu Li (Shandong University)
Chongyang Lv (Shandong University)
Xinzhi Liu (Southwest University)
Liping Li (Shanghai University of Finance and Economics)

Overview:
This replication package provides all the data and code necessary to reproduce the results of the paper. The package is designed to promote transparency and enable independent verification of all statistical results in the study.
DOI: 10.17632/6jmyj9g6rj.2
title: From Digital Infrastructure to Breakthrough Innovation: Evidence from China

description: README: Replication Package 
Paper Title: From Digital Infrastructure to Breakthrough Innovation: Evidence from China

Authors:
Peiyu Li (Shandong University)
Chongyang Lv (Shandong University)
Xinzhi Liu (Southwest University)
Liping Li (Shanghai University of Finance and Economics)

Overview:
This replication package provides all the data and code necessary to reproduce the results of the paper. The package is designed to promote transparency and enable independent verification of all statistical results in the study.
DOI: 10.17632/bpmrbntb3p
title: CyTOF profiling data of peripheral immune cells in mild ischemic stroke

description: This dataset contains raw mass cytometry (CyTOF) data of peripheral blood immune cells from patients with mild ischemic stroke and matched healthy controls. Peripheral blood samples were collected at days 1, 3, and 7 after stroke onset. The dataset includes immune cell profiling data used to characterize temporal and infarct location–specific differences in myeloid and lymphoid cell subsets, as well as immune checkpoint expression.
DOI: 10.17632/bpmrbntb3p.2
title: CyTOF profiling data of peripheral immune cells in mild ischemic stroke

description: This dataset contains raw mass cytometry (CyTOF) data of peripheral blood immune cells from patients with mild ischemic stroke and matched healthy controls. Peripheral blood samples were collected at days 1, 3, and 7 after stroke onset. The dataset includes immune cell profiling data used to characterize temporal and infarct location–specific differences in myeloid and lymphoid cell subsets, as well as immune checkpoint expression.
DOI: 10.5281/zenodo.18375257
title: GID raw data for DISDRODB

description: The dataset contains available disdrometer raw data of some of the stations of the GID network. The name of the folder is referred to the ID of the considered GID stations.  

Those data are used by DISDRODB software to download, process and archive disdrometer data of the considered stations following the best open science practices.

We respect to the previous version, here we added the data of the disdrometer installed in Torino (ID: TC-TO).