cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.48597/mdvf-v3ay
title: Arsenic at Kosetice (NOAK)

description: Measurement of Arsenic at Kosetice (NOAK)
DOI: 10.48597/muu3-gxsu
title: Inorganics in air and particle phase at Vavihill

description: Measurement of Inorganics in air and particle phase at Vavihill
DOI: 10.48597/n57u-mup6
title: Heavy metals at Aspvreten

description: Measurement of Heavy metals at Aspvreten
DOI: 10.48597/na42-4stj
title: Pm10_mass at Kosetice (NAOK)

description: Measurement of Pm10_mass at Kosetice (NAOK)
DOI: 10.48597/nt2m-3g57
title: Particle_number_size_distribution at Harwell

description: Measurement of Particle_number_size_distribution at Harwell
DOI: 10.48597/nxrf-f2yp
title: Inorganics in precipitation at Aspvreten

description: Measurement of Inorganics in precipitation at Aspvreten
DOI: 10.48597/p4m8-utz5
title: POPs (persistent organic pollutants) at Kosetice (NAOK)

description: Measurement of POPs (persistent organic pollutants) at Kosetice (NAOK)
DOI: 10.48597/pjzv-5eyw
title: Aerosol_light_scattering_coefficient at Zeppelin mountain (Ny-Ålesund)

description: Measurement of Aerosol_light_scattering_coefficient at Zeppelin mountain (Ny-Ålesund)
DOI: 10.48597/qaap-m2qx
title: Particle_number_size_distribution at Harwell

description: Measurement of Particle_number_size_distribution at Harwell
DOI: 10.48597/qbh7-r64q
title: Particle_number_size_distribution at Puy de Dôme

description: Measurement of Particle_number_size_distribution at Puy de Dôme
DOI: 10.48597/qhrj-zc3d
title: Heavy metals at Poiana Stampei

description: Measurement of Heavy metals at Poiana Stampei
DOI: 10.48597/raxq-3hda
title: Sulphate_corrected at Ispra

description: Measurement of Sulphate_corrected at Ispra
DOI: 10.48597/rd7k-9byn
title: Mercury at Bredkälen

description: Measurement of Mercury at Bredkälen
DOI: 10.6084/m9.figshare.19367178
title: Dataset from <i>Phytophthora capsici</i> sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity

description: The <i>de novo</i> biosynthesis of sterols is critical for the majority of eukaryotes; however, some organisms lack this pathway including most oomycetes. <i>Phytophthora</i> spp. are sterol auxotrophic but remarkably, have retained a few genes encoding enzymes in the sterol biosynthesis pathway. Here, we show that <i>PcDHCR7,</i> a gene in <i>Phytophthora capsici</i> predicted to encode Δ7-sterol reductase, displays multiple functions. When expressed in <i>Saccharomyces cerevisiae</i>, PcDHCR7 showed the Δ7-sterol reductase activity. Knocking out <i>PcDHCR7</i> in <i>P. capsici</i> resulted in the loss of the capacity to transform ergosterol into brassicasterol, which means PcDHCR7 has the Δ7-sterol reductase activity in <i>P. capsici</i> itself<i>.</i> This enables <i>P. capsici</i> to transform sterols recruited from the environment for better use. The biological characteristics of <i>ΔPcDHCR7</i> transformants were compared with those of the wild-type strain and a <i>PcDHCR7</i> complemented transformant, and the results showed that <i>PcDHCR7</i> plays a key role in mycelium development and pathogenicity of zoospores. Further analysis of the transcriptome indicated that the expression of many genes changed in the <i>ΔPcDHCR7</i> transformant, which involve in different biological processes. Possibly <i>P. capsici</i> compensates for the defects caused by the loss of <i>PcDHCR7</i> by remodelling its transcriptome.
DOI: 10.6084/m9.figshare.19367178.v1
title: Dataset from <i>Phytophthora capsici</i> sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity

description: The <i>de novo</i> biosynthesis of sterols is critical for the majority of eukaryotes; however, some organisms lack this pathway including most oomycetes. <i>Phytophthora</i> spp. are sterol auxotrophic but remarkably, have retained a few genes encoding enzymes in the sterol biosynthesis pathway. Here, we show that <i>PcDHCR7,</i> a gene in <i>Phytophthora capsici</i> predicted to encode Δ7-sterol reductase, displays multiple functions. When expressed in <i>Saccharomyces cerevisiae</i>, PcDHCR7 showed the Δ7-sterol reductase activity. Knocking out <i>PcDHCR7</i> in <i>P. capsici</i> resulted in the loss of the capacity to transform ergosterol into brassicasterol, which means PcDHCR7 has the Δ7-sterol reductase activity in <i>P. capsici</i> itself<i>.</i> This enables <i>P. capsici</i> to transform sterols recruited from the environment for better use. The biological characteristics of <i>ΔPcDHCR7</i> transformants were compared with those of the wild-type strain and a <i>PcDHCR7</i> complemented transformant, and the results showed that <i>PcDHCR7</i> plays a key role in mycelium development and pathogenicity of zoospores. Further analysis of the transcriptome indicated that the expression of many genes changed in the <i>ΔPcDHCR7</i> transformant, which involve in different biological processes. Possibly <i>P. capsici</i> compensates for the defects caused by the loss of <i>PcDHCR7</i> by remodelling its transcriptome.
DOI: 10.6084/m9.figshare.8357186
title: MOESM1 of Identification of potential key genes and pathways predicting pathogenesis and prognosis for triple-negative breast cancer

description: Additional file 1: Table S1. Differential expressed mRNAs between 1109 breast cancer tissue samples and 113 normal tissue samples. Differential expressed mRNAs between 117 TNBC and 970 non-TNBC breast cancer samples. 590 up-regulated overlapped DEGs and 165 down-regulated overlapped DEGs.
DOI: 10.6084/m9.figshare.8357186.v1
title: MOESM1 of Identification of potential key genes and pathways predicting pathogenesis and prognosis for triple-negative breast cancer

description: Additional file 1: Table S1. Differential expressed mRNAs between 1109 breast cancer tissue samples and 113 normal tissue samples. Differential expressed mRNAs between 117 TNBC and 970 non-TNBC breast cancer samples. 590 up-regulated overlapped DEGs and 165 down-regulated overlapped DEGs.
DOI: 10.5281/zenodo.10730822
title: PERCEPTIONS AND ADOPTION OF DIFFERENTIATED INSTRUCTION BY HIGH EDUCATION TEACHERS
DOI: 10.5281/zenodo.10730821
title: PERCEPTIONS AND ADOPTION OF DIFFERENTIATED INSTRUCTION BY HIGH EDUCATION TEACHERS
DOI: 10.6084/m9.figshare.19367243
title: Additional file 1 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 1. Quantitative tool: quantitative questionnaire for the population. QuantiPop.
DOI: 10.6084/m9.figshare.19367243.v1
title: Additional file 1 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 1. Quantitative tool: quantitative questionnaire for the population. QuantiPop.
DOI: 10.6084/m9.figshare.19367246
title: Additional file 2 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 2. Quantitative tool: questionnaire for the health service providers. QuantiHSP.
DOI: 10.6084/m9.figshare.19367246.v1
title: Additional file 2 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 2. Quantitative tool: questionnaire for the health service providers. QuantiHSP.
DOI: 10.6084/m9.figshare.25323766
title: A Systematic Analysis on the Application Areas of Cognitive Load Theory

description: A Systematic Analysis on the Application Areas of Cognitive Load Theory <br>
DOI: 10.6084/m9.figshare.25323766.v1
title: A Systematic Analysis on the Application Areas of Cognitive Load Theory

description: A Systematic Analysis on the Application Areas of Cognitive Load Theory <br>
DOI: 10.6084/m9.figshare.19367255
title: Additional file 5 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 5.
DOI: 10.6084/m9.figshare.19367255.v1
title: Additional file 5 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 5.
DOI: 10.48597/rj9w-dzs5
title: Lead at Poiana Stampei

description: Measurement of Lead at Poiana Stampei
DOI: 10.48597/sat8-pvhj
title: Aerosol_light_backscattering_coefficient at Pallas (Sammaltunturi)

description: Measurement of Aerosol_light_backscattering_coefficient at Pallas (Sammaltunturi)
DOI: 10.48597/sgtv-bqe5
title: Particle_number_size_distribution at Mace Head

description: Measurement of Particle_number_size_distribution at Mace Head
DOI: 10.48597/smv4-c5sw
title: Particle_number_size_distribution at Harwell

description: Measurement of Particle_number_size_distribution at Harwell
DOI: 10.48597/tdtq-nq5e
title: PM mass at Vavihill

description: Measurement of PM mass at Vavihill
DOI: 10.48597/ttzy-xsww
title: Particle_number_concentration at Hohenpeissenberg

description: Measurement of Particle_number_concentration at Hohenpeissenberg
DOI: 10.48597/ubyh-sfrt
title: Arsenic at Kosetice (NOAK)

description: Measurement of Arsenic at Kosetice (NOAK)
DOI: 10.48597/ukhj-swhc
title: Particle_number_size_distribution at Mace Head

description: Measurement of Particle_number_size_distribution at Mace Head
DOI: 10.48597/vemg-duc3
title: Lead and manganium at Svratouch

description: Measurement of Lead and manganium at Svratouch
DOI: 10.48597/vncc-cadf
title: Particle_number_size_distribution at Mt Cimone

description: Measurement of Particle_number_size_distribution at Mt Cimone
DOI: 10.48597/w3yb-r7nm
title: Aerosol_optical_depth at Potsdam

description: Measurement of Aerosol_optical_depth at Potsdam
DOI: 10.48597/wsmk-8zjy
title: Inorganics in air and particle phase at Sniezka

description: Measurement of Inorganics in air and particle phase at Sniezka
DOI: 10.48597/xm9d-52yz
title: Particle_number_size_distribution at Kosetice (NOAK)

description: Measurement of Particle_number_size_distribution at Kosetice (NOAK)
DOI: 10.48597/xvxw-8nmk
title: Ozone at Kosetice (NAOK)

description: Measurement of Ozone at Kosetice (NAOK)
DOI: 10.48597/ybxb-mpvy
title: Particle_number_size_distribution at Cabauw Zijdeweg

description: Measurement of Particle_number_size_distribution at Cabauw Zijdeweg
DOI: 10.48597/ydsg-uht6
title: Aerosol_optical_depth at Zugspitze-Gipfel

description: Measurement of Aerosol_optical_depth at Zugspitze-Gipfel
DOI: 10.6084/m9.figshare.19367264
title: Additional file 8 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 8.
DOI: 10.48597/yrhr-85bg
title: Pm10_mass at Aspvreten

description: Measurement of Pm10_mass at Aspvreten
DOI: 10.48597/zdyz-kv86
title: Particle_number_size_distribution at Zugspitze-Schneefernerhaus

description: Measurement of Particle_number_size_distribution at Zugspitze-Schneefernerhaus
DOI: 10.48597/22uu-5k32
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/zftn-zghp
title: Copper at Kosetice (NOAK)

description: Measurement of Copper at Kosetice (NOAK)
DOI: 10.6084/m9.figshare.19367264.v1
title: Additional file 8 of Healthcare system resilience in Bangladesh and Haiti in times of global changes (climate-related events, migration and Covid-19): an interdisciplinary mixed method research protocol

description: Additional file 8.
DOI: 10.48597/23yc-bfve
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/2vxh-3avj
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/2zez-6ug8
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/332y-zvcs
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/37wg-chwe
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/38df-3fbu
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/3pzx-g4w7
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/3s66-24gf
title: Measurement of Heavy metals at Kollumerwaard

description: Measurement of Heavy metals at Kollumerwaard
DOI: 10.48597/3wqg-hv9x
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/43fb-k4w4
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/44nf-wysk
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/468z-6duk
title: Measurement of Ozone at Öhringen

description: Measurement of Ozone at Öhringen
DOI: 10.48597/4dap-mtjs
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/4njy-hu48
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/4pt9-f9xg
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/4sn7-tvpc
title: Measurement of Heavy metals at Kollumerwaard

description: Measurement of Heavy metals at Kollumerwaard
DOI: 10.48597/52eg-48bt
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/5423-fks8
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/5jcv-45d8
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/5kt4-weg8
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/5zzv-aqe7
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/65sp-p8am
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/66pr-ze7m
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/6hvc-v3fs
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/6syz-aen9
title: Measurement of Heavy metals at Kollumerwaard

description: Measurement of Heavy metals at Kollumerwaard
DOI: 10.48597/6ut7-c2x3
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/6xxd-gby4
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/6zea-8s8u
title: Measurement of Heavy metals at Kollumerwaard

description: Measurement of Heavy metals at Kollumerwaard
DOI: 10.48597/72md-hr5j
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/7can-3uzp
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/7qpr-uzre
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/84ge-uvw8
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/8aae-7fsk
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/8fgv-rdw4
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/8z7q-qtht
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/9659-ttgy
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/9rfu-6wkh
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/9uek-ngax
title: Measurement of Calcium at Kollumerwaard

description: Measurement of Calcium at Kollumerwaard
DOI: 10.48597/bctm-av8s
title: Measurement of Precipitation_amount at Kollumerwaard

description: Measurement of Precipitation_amount at Kollumerwaard
DOI: 10.48597/bpj7-m82e
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/bu9j-e9wf
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/c3kx-gfp3
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/c8df-ft8g
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/cbgu-wumy
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/cgef-qdfh
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/d2x3-7z6k
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/d9pg-a873
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.5281/zenodo.10466561
title: Learning Path Data

description: The given data set contains learning path data in combination with ILS strength expressions.An example entry for the Active learning style looks like:




ILS Strength
LE1
LE2
LE3
LE4
LE5
LE6
LE7
LE8
LE9


7
LG
BO
MS
AAM
QU
EX
VAM
QU
LG




The learning elements are chosen according to the learning element defined by Staufer et al. in "LEARNING ELEMENTS IN ONLINE LEARNING MANAGEMENT SYSTEMS" (doi:10.21125/iceri.2023.0815)
DOI: 10.5281/zenodo.10466562
title: Learning Path Data

description: The given data set contains learning path data in combination with ILS strength expressions.An example entry for the Active learning style looks like:




ILS Strength
LE1
LE2
LE3
LE4
LE5
LE6
LE7
LE8
LE9


7
LG
BO
MS
AAM
QU
EX
VAM
QU
LG




The learning elements are chosen according to the learning element defined by Staufer et al. in "LEARNING ELEMENTS IN ONLINE LEARNING MANAGEMENT SYSTEMS" (doi:10.21125/iceri.2023.0815)
DOI: 10.48597/dcca-gwhz
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/dp3q-dz6s
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/due8-d2nr
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard
DOI: 10.48597/ecbq-zenf
title: Measurement of Sulphate_corrected at Kollumerwaard

description: Measurement of Sulphate_corrected at Kollumerwaard
DOI: 10.48597/ey6d-zjxu
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/ff78-3mfn
title: Measurement of Inorganics in precipitation at Kollumerwaard

description: Measurement of Inorganics in precipitation at Kollumerwaard
DOI: 10.48597/fudx-j2gw
title: Measurement of Heavy metals at Kollumerwaard

description: Measurement of Heavy metals at Kollumerwaard
DOI: 10.48597/gzjs-nmbs
title: Measurement of Conductivity at Kollumerwaard

description: Measurement of Conductivity at Kollumerwaard
DOI: 10.48597/h534-a9wu
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.6084/m9.figshare.12027249
title: Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress

description: Abstract The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19 stress-related genes. RNA interference used to further assess the functions of the cold-related unigenes 15628 and 15596 showed that the knockdown of each of these genes led to decreased cold tolerance of D. destructor. Hence, this study revealed molecular processes and pathways active in cold- or dessication-treated nematodes. The transcriptome profiles presented in this study provide insight into the transcriptome complexity and will contribute to further understand stress tolerance in D. destructor.
DOI: 10.48597/hwdh-r5xu
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.48597/jg96-jmh9
title: Measurement of Calcium at Kollumerwaard

description: Measurement of Calcium at Kollumerwaard
DOI: 10.48597/jgp7-jcn5
title: Measurement of pH at Kollumerwaard

description: Measurement of pH at Kollumerwaard
DOI: 10.6084/m9.figshare.12027249.v1
title: Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress

description: Abstract The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19 stress-related genes. RNA interference used to further assess the functions of the cold-related unigenes 15628 and 15596 showed that the knockdown of each of these genes led to decreased cold tolerance of D. destructor. Hence, this study revealed molecular processes and pathways active in cold- or dessication-treated nematodes. The transcriptome profiles presented in this study provide insight into the transcriptome complexity and will contribute to further understand stress tolerance in D. destructor.
DOI: 10.48597/kkb2-qkhb
title: Measurement of Nitrogen_dioxide at Kollumerwaard

description: Measurement of Nitrogen_dioxide at Kollumerwaard