DOI: 10.22002/mgzq9-fgd83
title: NR1769pKM101+NR1769

description: Skinny E. coli NR1769 with conjugative plasmid plus skinny E. coli NR1769
description: <strong>Tilt Series Date:</strong> 2014-11-25</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> NR1769 pKM101</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christian Baron</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown to log phase and mixed 1:1 ratio for 30 min to induce conjugation</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/rawdata/</p>4plus7_M130005.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/rawdata/4plus7_M130005.mrc"
        > <i class="download icon"></i> Download </a></p>

        4plus7_M130005.rec,  Reconstruction (Pixel Size 0.0 nm), 711.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/3dimage_49630/4plus7_M130005.rec"
        > <i class="download icon"></i> Download </a></p>

        slicer000.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12173/slicer000.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer001.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12174/slicer001.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer002.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12175/slicer002.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer003.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12176/slicer003.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer004.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12177/slicer004.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer005.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12178/slicer005.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer006.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12179/slicer006.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer007.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12180/slicer007.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer008.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12181/slicer008.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer009.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12182/slicer009.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer010.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12183/slicer010.tif"
        > <i class="download icon"></i> Download </a></p>

        slicer011.tif,  2.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-25-5/file_12184/slicer011.tif"
        > <i class="download icon"></i> Download </a></p>