DOI: 10.5061/dryad.15dv41p8f
title: Squash preparations and living cells showing chromosome arrangement in mitosis, meiosis I, and meiosis II in green lacewing (Chrysoperla rufilabris) males

description: In previously-studied species of insects in the order Neuroptera, sex
 chromosomes in males exhibit a phenomenon called "distance
 segregation," where the X and Y chromosomes are separated and
 associated with opposite poles from prometaphase I-anaphase I. This study
 aimed to determine the chromosome number and sex determination of a
 previously unstudied Neuropteran insect, the green lacewing Chrysoperla
 rufilabris (Burmeister, 1839).  Following determination of
 chromosome number and sex determination we examined images of fixed and
 living specimens to determine if distance segregation was observed. This
 dataset includes all of the raw images of living and aceto-orcein squashed
 cells that were observed to determine chromosome number, sex
 determination, and existence of distance segregation.
description: Testes of <em>Chrysoperla rufilabris</em> were
 removed from adult male abdomens.   For aceto-orcein
 squashes, isolated testes were fixed in 3:1 ethanol/acetic acid for 5
 minutes, rinsed 30 seconds in distilled water, and stained in filtered
 aceto-orcein (2.5 g orcein in 100 mL 45% (v/v) acetic acid) for 3
 minutes.  Stained testes were placed on a slide in a drop of 45% acetic
 acid.  A coverslip was placed over the testes and uniform pressure was
 applied by hand on the coverslip.  For living-cell
 preparations, testes were placed under a thin layer of Kel-F #10 oil (Ohio
 Valley Specialty Company, Marietta, Ohio) in a culture chamber on a
 coverslip.  Testes contents were spread under the oil and living cells
 were immediately imaged. Imaging was done on a Nikon
 Eclipse TS100 microscope (Nikon Instruments, Tokyo, Japan) equipped with a
 100X, 1.25 NA phase-contrast, oil immersion objective.  Images were
 collected either with a View 4K HD camera (Microscope Central,
 Feasterville, PA, USA), using InFocus software version x64 (Microscope
 Central), or using a Spot camera with the Spot 3.5.7 software (SPOT
 Imaging, Sterling Heights, MI, USA).
description: # Squash preparations and living cells showing chromosome arrangement in
 mitosis, meiosis I, and meiosis II in green lacewing (*Chrysoperla
 rufilabris*) males Dataset DOI:
 [10.5061/dryad.15dv41p8f](10.5061/dryad.15dv41p8f) ## Description of the
 data and file structure This dataset contains the raw image files required
 to study chromosome number, alignment, and position during mitosis,
 meiosis I, and meiosis II in adult males of the green lacewing
 *Chrysoperla rufilabris* (Burmeister, 1839).  Images of aceto-orcein
 squashes of testes and living spermatocytes are included in this dataset. 
 Preparations were imaged using a Nikon Eclipse TS100 microscope equipped
 with a 100X, 1.25 NA phase-contrast, oil immersion objective.  Images of
 aceto-orcein squashes were collected using a View 4K HD camera (Microscope
 Central, Feasterville, PA, USA) using InFocus software version x64
 (Microscope Central).  Images of living cells were either collected  using
 a View 4K HD camera using InFocus software version x64 (subfolder
 03-19-2025), or a Spot camera with the Spot 3.5.7 software (SPOT Imaging,
 Sterling Heights, MI, USA).  In images in the subfolder "Live-cell
 preps" in the sub-subfolders 02-19-2009, 04-14-2009, and 07-21-2009,
 130 pixels=10 μm.  In images in the subfolder "Live-cell preps"
 in the sub-subfolder 03-19-2025, 620 pixels=10 μm.  In all images in the
 subfolder "Chromosome-squash preps" 620 pixels=10 μm. ### Files
 and variables #### File: C-rufilabris-male-cell-division_Dryad-upload.zip
 **Description:** The zipped folder
 (C-rufilabris-male-cell-division_Dryad-upload_07-21-2025.zip) contains
 3490 image files, all in jpeg format.  The images are all of testes
 preparations from male *Chrysoperla rufilabris*. Subfolder
 "Chromosome-squash preps" contains 778 files organized into 14
 sub-subfolders that are named according to the date images sub-subfolder
 were obtained, in the format MM-DD-YYYY where M=month, D=day, and Y=year. 
 Images of squashed cells in mitosis, meiosis I, and meiosis II are
 contained in these sub-subfolders.  Within the sub-subfolders, the file
 naming convention is based on date/time imaged.  File names are in the
 format YYYYMMDDHHMMSSsss, where Y=year, M=month, D=day, H=hour, S=second,
 and s=millisecond. Subfolder "Live-cell preps" contains 2496
 files organized into 4 sub-subfolders named in the format MM-DD-YYYY where
 M=month, D=day, and Y=year.  All images in this subfolder are of living
 prometaphase I and metaphase I spermatocytes.  File naming conventions are
 as follows: In the "02-19-2009" sub-subfolder, images in the
 "cell 01" sub-sub-subfolder are named with the prefix cell01
 followed by the number in the series starting with 000 and ending with
 785. In the "03-19-2025" sub-subfolder, all image file names are
 in the format YYYYMMDDHHMMSSsss, where Y=year, M=month, D=day, H=hour,
 S=second, and s=millisecond. In the "04-14-2009" sub-subfolder,
 all images are named with the prefix "cell01" followed by the
 number in the series starting with 000 and ending with 292. In the
 "07-21-2009" sub-subfolder, within the "Cell 01"
 sub-sub-subfolder all images are named with the prefix "Cell
 one" followed by the number in the series, starting with 000 and
 ending with 1030. ## Code/software Images can be viewed using any image
 viewer.  Images can be viewed and analyzed using ImageJ
 ([https://imagej.net/ij/](https://imagej.net/ij/)) ## Access information
 Other publicly accessible locations of the data: * NA Data was derived
 from the following sources: * NA