DOI: 10.6084/m9.figshare.7588181
title: Data related to Fabrication of innocuous gold nanoparticles using plant cells in culture – A novel approach, including particle size analysis, catalytic activity and toxicology

description: This data set contains data on PCR primers, FACS analysis, cytotoxicity, particle size analysis, catalytic activity, chromatography, MTT assay and infrared spectroscopy, created and analysed during a study of the fabrication of innocuous gold nanoparticles using plant cells in culture.<br>The related research article describes the development and testing of a novel method for the fabrication of gold nanoparticles (AuNPs) using cell suspension culture of the plant <i>Medicago sativa. </i>This technique is designed as a cost effective and environmentally friendly alternative to physical and chemical methods of nanomaterial synthesis. <i>M. Sativa</i> is utilised for its property of accumulating heavy metals, where the use of cell suspension culture provides useful controlled conditions for particle isolation, compared to the more laborious process of growing entire plants under controlled conditions for this purpose.<br>As toxicity is a significant aspect of this process, often dependent on physical dimensions and surface chemistry of AuNPs, the immunological response to derived nanoparticles in this study was also investigated in a murine model.<b><br></b><b>Methods</b><br>This section provides background to the files held in this data record, for a detailed description of the study methods see the related publication.<br><i>M. Sativa </i>hypocotyl regions were extracted into a continually growing plant cell culture, a single cell suspension culture was then tested for KAuCl<sub>4</sub> tolerance, before supplementing with KAuCl<sub>4 </sub>for AuNP synthesis and recovery of AUNPs, including FT-IR spectroscopy to detect particle presence. Absorbance analysis was then performed for testing of catalytic activity and MTT assay testing for cytotoxicity of nanoparticles in human and mouse cell lines. Morphological analysis of particles was performed via Electron microscopy and cytokine detection in lab mice via ELISA.<br><br><b>Data set contents:</b><br><b>Real time PCR primers.docx</b> - MS Word file holding PCR primers for transcript analysis.<b>Absorbance of column fractions.xls - </b>MS Excel spreadsheet holding absorbance of ten eluted column fractions vs wavelength in nm. Wavelength shift data per fraction is held in a separate tab.<b>Catalytic activity.xlsx </b> - MS Excel spreadsheet holding absorbance data at wavelengths 500-800 nm for five minutes intervals over one hour for test and control groups (note these are separate tabs, with a summary tab comparing overall results).<b>Cytokine profile mice.xlsx - </b>MS Excel spreadsheet holding levels of cytokines and growth factors in control and test mouse groups in <b>Sheet1</b>, ANOVA and t-test analysis in <b>Stats </b>tab.<b>FACS analysis.pptx - </b>MS PowerPoint file of fluorescence-activated cell sorting results, including effects of nanpoarticles 1 and 2 on different cell lines.<b>FTIR dry ccGNP Oct9.xls - </b>MS Excel spreadsheet holding Fourier-transform infrared spectroscopy results in % transmittance against wave number.<b>KAUCl4 cytotoxicity.xls - </b>MS Excel spreadsheet holding cytotoxicity analysis including survival rates in trials 1 and 2 in separate tabs, along statistical analysis in terms of t-test and ANOVA on sheets 1 and 2.<b>MTT assay.pptx - </b>MS Excel spreadsheet holdingpowerpoint file of MTT colorimetric assay, including effects of nanpoarticles 1 and 2 on different cell lines.<b>Particle size analysis.xls - </b>MS Excel spreadsheet holding<b> </b>nanoparticle particle size in terms of diameter, perimeter and feret diameter. Tabs are labelled A-J corresponding to concentrations of KAuCl4 in ppm outlined in the stats and graph tabs, including a summary tab for each.<b>PH-shape Particle analysis.xls - </b><b> </b>MS Excel spreadsheet holding proportions (in percent) of different nanoparticle shapes at different pH values.<b>RT-PCR mice result.xls - </b>MS Excel spreadsheet holding real time PCR results per cytokine (in individual tabs), for cell culture synthesized nanoparticles (ccAuNPs) and chemically synthesized nanoparticles (csAuNPs).<br>The animal use protocol followed in the investigation was reviewed and approved by Western Kentucky University's Institutional Animal Care and Use Committee (Animal Welfare Assurance # A3558-01) on December 8, 2016: “Assessment of Plant-derived, Ecofriendly Gold Nanoparticles using an Animal System.” This protocol was assigned the following designation (16-09).<br>