DOI: 10.5061/dryad.xksn02vq0
title: Environmentally-associated color divergence does not coincide with population structure across Lesser Antillean anoles

description: Species distributed across heterogeneous environments may undergo local
 adaptation, which can be limited by the homogenizing effects of gene flow.
 Lesser Antillean anoles exhibit dorsal color variation associated with
 dramatic shifts in environment across small spatial scales, providing an
 excellent system for studying the maintenance of local adaptation despite
 ongoing gene flow. The 1995 Soufriere Hills eruption on Montserrat
 provides an opportunity to understand how natural disasters may influence
 evolution of insular species. Thus, our study had two main objectives.
 First, we collected genetic, phenotypic, and environmental data to
 investigate whether environmentally-associated dorsal coloration reflects
 underlying population structure in three species of Lesser Antillean
 anoles. Second, we examined the short-term evolutionary consequences of
 volcanism by comparing pre- and post-eruption samples of Anolis lividus
 using multi-locus sequence data. We found all three species exhibited
 genetic isolation- by-distance but not isolation-by-environment.
 Furthermore, phenotypic differences within each species were not predicted
 by genetic distances, suggesting gene flow across color morphs. We
 confirmed the repopulation of A. lividus in the impacted area and found
 genetic diversity equivalent to pre-eruption sampling. Our findings
 contribute to our understanding of local adaptation in spatially
 small-scale systems and the impact of catastrophic natural disasters on
 population structure.
description: # README: Environmentally-associated color divergence does not coincide
 with population structure across Lesser Antillean anoles Access this
 dataset on Dryad:
 [https://doi.org/10.5061/dryad.xksn02vq0](https://doi.org/10.5061/dryad.xksn02vq0) Submitted here is a datafile containing the unphased DNA sequence of a nuclear gene (MC1R) in FASTA format. Sequences were generated by Sanger sequencing. These sequences are provided here because they do not meet the minimum length required for accession in Genbank. These data were used in analyses described in the article "Environmentally-associated color divergence does not coincide with population structure across Lesser Antillean anoles." In this study we examined the relationship between genetic population structure and habitat associated phenotypic differences in Lesser Antillean anoles. Specifically, MC1R data is derived from *Anolis lividus* from the island of Montserrat. ## Description of the data and file structure Data is provided in standard FASTA format. The datafile contains sequence data from 59 individuals of *Anolis lividus* for the nuclear gene MC1R. Data are aligned and unphased. All sequences are 163 base pairs in length. Anolis-lividus_MC1R_163bp.fasta File Details: * Name: Anolis-lividus_MC1R_163bp.fasta * Format: FASTA File Data Description: In FASTA format individual sequences begin with "&gt;" followed by the name of the sequences. Here, sequences are named by field collection ID (collector initials followed by numeric identifier) or by the specimen accession number if derived from a natural history museum's tissue collection. The sequence name is followed by a line break and then sequence data in a single line. Sequence data is represented using IUPAC ambiguity codes. This data file is compatible with a wide variety of analytical software that accepts FASTA formatted files.  ## Sharing/Access information All other sequence data are available on Genbank under the following accession numbers: PP195265–PP195577.