DOI: 10.5061/dryad.fj852
title: Data from: Destabilizing mutations encode nongenetic variation that drives evolutionary innovation

description: Evolutionary innovations are often achieved by repurposing existing genes
 to perform new functions; however, the mechanisms enabling the transition
 from old to new remain controversial. We identified mutations in
 bacteriophage λ’s host-recognition gene J that confer enhanced adsorption
 to λ’s native receptor, LamB, and the ability to access a new receptor,
 OmpF. The mutations destabilize particles and cause conformational
 bistability of J, which yields progeny of multiple phenotypic forms, each
 proficient at different receptors. This work provides an example of how
 nongenetic protein variation can catalyze an evolutionary innovation. We
 propose that cases where a single genotype can manifest as multiple
 phenotypes may be more common than previously expected and offer a general
 mechanism for evolutionary innovation. 
description: Petrie Science 2018 Fig
 1B and S1Data collected for
 results shown in figure 1B and S1, see notes within the file and the
 manuscript for further guidanceDryad Petrie Science 2018 Fig
 1B and S1.csvPetrie Science 2018 Fig
 1C and S2Data collected for
 results shown in figures 1C and S2, see notes within the file and the
 manuscript for further guidance.Petrie Science Fig 1C and
 S2.csvPetrie Science 2018
 sequence of 3-mut replicate 1Sequence of engineered
 3-mut J gene replicate 1 (see table S1 for additional
 guidance).3-Mut_4-Jrev.seqPetrie 2018 Science sequence of 3-mut replicate 2.Sequence of engineered 3-mut J gene replicate 2 (see table S1 for additional guidance).3-Mut_7-Jrev.seqPetrie Science 2018 sequence of 4-mut replicate 1Sequence of engineered 4-mut J gene replicate 1 (see table S1 for additional guidance).4-MUT (P5_03-JRev).seqPetrie Science 2018 sequence of 4-mut replicate 2Sequence of engineered 4-mut J gene replicate 2 (see table S1 for additional guidance).4-MUT (P5_04-JRev).seqPetrie Science 2018 sequence of 5-mut replicate 1Sequence of engineered 5-mut J gene replicate 1 (see table S1 for additional guidance).5-MUT (Mage01_1-JRev).seqPetrie Science 2018 sequence of 5-mut replicate 2Sequence of engineered 5-mut J gene replicate 2 (see table S1 for additional guidance).5-MUT (Mage01_2-JRev).seqPetrie Science 2018 Sequence of engineered 7-mut lyso.Sequence of engineered 7-mut lyso J gene (see table S1 for additional guidance)31F1_1a-J_Rev.ab1-1.faPetrie Science 2018 Fig 2AData collected for results shown in figure 2A, see notes within the file and the manuscript for further guidance.Petrie Science 2018 Fig 2BData collected for results shown in figure 2B, see notes within the file and the manuscript for further guidance.Petrie Science 2018 Fig 2CData collected for results shown in figure 2C, see notes within the file and the manuscript for further guidance.Petrie Science 2018 Fig 3A and 3BData collected for results shown in figures 3A and 3B, see notes within the file and the manuscript for further guidance.Petrie Science 2018 Fig 3CData collected for results shown in figure 3C, see notes within the file and the manuscript for further guidance.Petrie Science Fig 3DData collected for results shown in figure 3C, see notes within the file and the manuscript for further guidance.Petrie Science 2018 Fig 3D.csvPetrie Science 2018 Fig S4A and S4BData collected for results shown in figure S4A and S4B, see notes within the file and the manuscript for further guidance.Petrie Science 2018 Fig S5Data collected for results shown in figure S5, see notes within the file and the manuscript for further guidance.