cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0571bcv
title: CIL:39140, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The cytosol of Paramecium contains many vesicles of various sizes, shapes and contents as well as complex particles such as ribosomes and glycogen granules. The rough endoplasmic reticulum exists as membrane cisternae and tubules which bear ribosomes on their cytosolic surface. Mitochondria and peroxisomes both have tubular membrane inclusions. Large vesicles with an electron transparent center are acidosomes. Lysosomes have a heterogeneous content but always have a heavy polysaccharide lining on their luminal membrane face. Flattened vesicles, called discoidal vesicles, form a pool of membrane from which phagosome membrane is derived. TEM taken on 10/30/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X.
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0d50m9c
title: CIL:39139, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Receptor mediated endocytosis is a process in which extracellular molecules are taken into the cell via coated vesicles that pinch from the plasma membrane. In Paramecium the resulting vesicles fuse with flattened cisterna called early endosomes. Early endosomes accumulate just under the cortex of the cell. Sorting of proteins destined to be returned to the plasma membrane after separating from cargo occurs in the early endosomes and is facilitated by the formation of secondary coated pits and vesicles on the early endosomes that are smaller than the coated and uncoated preendosomal vesicles that arise from the plasma membrane. These small coated pits pinch off and subsequently lose their coats to form carrier vesicles that are roughly 100nm in diameter. These vesicles move deeper into the cell where they bind to microtubular ribbons. TEM taken on 11/4/68 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X.
DOI: 10.6075/j0sf2vgf
title: CIL:39138, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Tangential section through the anterior half of the cytopharyngeal basket. Drawings and discussion of the basket can be found in a paper by Tucker, J. Cell Science 3: 493-514, 1968. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV.
DOI: 10.6075/j0qj7gnf
title: CIL:39137, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The top of the cytopharyngeal basket showing a closed cytostome where food will be engulfed. In the lab Nassula was fed Phormidium inundatum, a cyanobacterium. Ingested food is seen in food vacuoles as intensely stained blobs which are in various stages of digestion. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X.
DOI: 10.6075/j00001d7
title: CIL:39208, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: A micrograph showing the polyploidal macronucleus of Tetrahymena containing many copies of the genome. Chromatin material is found as condensed electron opaque spots as well as in an uncondensed form between the opaque spots. The macronucleus, unlike the micronucleus, contains many nucleoli that tend to accumulate next to the nuclear envelope. Nucleoli are sites of ribosomal RNA synthesis and ribosome subunit assembly. TEM taken on 8/14/67 by R. Allen with Philips 200 operating at 60kV. Neg. 4,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0mw2ggt
title: CIL:39205, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Nucleoli in a macronucleus of a cell undergoing micronuclear division. The nucleolus appears to have an organized surface layer composed of parallel threads of subunits. These may be developing ribosomal subunits that will soon be transported to the cytoplasm through the nuclear pores. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 15,759X.
DOI: 10.6075/j05t3jts
title: CIL:39204, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: The micronucleus of Paramecium caudatum is distinctive from other closely related Paramecium such as P. multimicronucleatum and P. tetraurela. P. caudatum has only one micronucleus of a type in which the chromatin is spread equally throughout the nucleus as opposed to P. multimicronucleatum which has four very small micronuclei of the vesicular type and P. aurelia which has two micronuclei of the vesicular type where the chromatin forms a central electron opaque mass in the center of the nucleus. The micronucleus in this micrograph contains many microtubules so it may be in early division. The nuclear envelope has many nuclear pores. TEM taken on 3/23/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,250X.
DOI: 10.6075/j05b0136
title: CIL:39201, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: A bundle of microtubules that arises from a basal body adjacent to the cytoproct comes to lie along a spent DV-IV. The assumption is that movement generated by molecular motors propels the spent vacuole toward the cytoproct. TEM taken on 6/1/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Published in J. Cell Sci. 14:611-631, 1974. Adapted with permission.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j02b8xd3
title: CIL:39157, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Detail of the junction between the ribbed wall and the right side of the cytopharynx area. The ribbed wall is covered by alveoli and the ribs are produced by small narrow ridges similar to the larger ridges covering the whole cell body. Two arrays of microtubules are present in this zone, clumps of hexagonally packed bundles lying under the ridges and rows of microtubules that lie under the single cytopharyngeal membrane. There is some suggestion that these rows of microtubules may associate together to form the bundles. Tips of cilia in cross section give 4 views of how the tips of cilia end, the 9-doublet first become 9 singlets and then these are lost one by one leaving only the two central singlets at the tip of a cilium. Sections of the cytostomal cord are also present under the rib wall. This is the cord that wraps around the cytopharynx. TEM taken on 7/27/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 23,000X.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0bp0242
title: CIL:39156, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Cross section through the more anterior part of the oral region showing the four more loosely spaced rows of cilia making up the quadrulus and more compact cilia of the peniculi on the left hand wall of the buccal cavity. The indentation of the endoral membranelle lies to the right just above the buccal overture. The vestibulum bearing somatic ciliature connects the buccal cavity with the outside of the cell. The ribbed wall covers the upper right side of the buccal cavity. mac, macronucleus. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,850X.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j07w6bhq
title: CIL:39155, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Developing trichocyst containing a paracrystalline core surrounded by amorphous material and an autophagosome containing mitochodrion. TEM taken on 1/25/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0nc601v
title: CIL:38928, Cucurbita, plant cell, xylem, phloem. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: modes of light microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in intrinsic optical density
Processing history: unprocessed raw data
description: B0006199 Vascular bundles in a marrow stem. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Unlike humans and animals, plants use separate systems to transport food and water - the heavily reinforced xylem for transporting water and the phloem system, with its own built in filters (sieve plates) for food. In this longitudinal section of part of a marrow (Cucurbita) stem, the red bands show the reinforcing structures in the xylem, and the lighter brown vertical bands across the vessels at the top of the image show the sieve plates arranged at intervals along the phloem.
DOI: 10.6075/j0fj2fm6
title: CIL:38927, Rhizopus, bread mold. In Cell Image Library

description: B0006132 Fruiting body of bread mould. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Scanning electron micrograph of the the fruiting body of bread mould. As the mould grows, it propagates itself by forming spores that are released into the environment and grow into new mould colonies.
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j0z89b81
title: CIL:39001, sensory neuron. In Cell Image Library

description: Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
description: B0001893 Chemorepulsion of DRG sensory neuron. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Sensory neuron from an adult dorsal root ganglion immunostained for Growth Associated Protein (GAP43) shown in red. Neurites are growing in between stripes containing the EphrinA5 protein (green). When the cell processes come into contact with this protein they are repelled and therefore grow along between the stripes. The effects of the Ephrin molecules are mediated through a family of tyrosine kinase receptors and are important in providing repulsive signals during the development of the nervous system
DOI: 10.6075/j0np238t
title: CIL:38993, blood cell, red blood cell, white blood cell. In Cell Image Library

description: Scanning electron microscope image of a blood clot showing a white blood cell in the center.
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: B0000562 Electron micrograph of blood clot,high powe. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
DOI: 10.6075/j0b85703
title: CIL:38992, Danio rerio, neuromast hair cell. In Cell Image Library

description: Scanning electron micrograph of the apical surface of neuromast organ of lateral line system in a 17-day-old zebrafish. The hair bundles at the top of each sensory cell emerge through a pore in the skin (picture width 52.8 um). Movement of the surrounding water deflects the hair bundle, stimulating the hair cell. Fish (and aquatic amphibian larvae) use such info to detect presence of other fish or avoid predators. All vertebrate inner ears that detect sound vibrations and are similar to the hair cells of neuromast organs.
description: Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: B0002042 Neuromast organ, lateral line system. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/