cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.6276620
title: ENHG Annotation of "Verleihbuch der Rattenberger Bergrichter" (TLA Hs. 37, 1460-1463)

description: The dataset contains the TEI tags of the historical mining document "Verleihbuch der Rattenberger Bergrichter" (TLA Hs. 37, 1460-1463) that is currently stored in the Tyrolean Regional archives (Innsbruck). The citeable Transcript is online available (DOI: 10.10.5281/zenodo.6274928) as well as the related annotation guidelines (DOI: 10.5281/zenodo.6275197) The data was generated by the research team of the project “Text Mining Medieval Mining Texts” (T.M.M.M.T.). The research project (2019-2022) was carried out at the University of Innsbruck and funded by go!digital next generation programme of the Austrian Academy of Sciences.
DOI: 10.5281/zenodo.6275763
title: Historical Place Gazetteer and Historical Mine Register, Tyrol 15th, 16th century

description: The dataset is split in two separated files in CSV format (UTF 8): 1) Historical place gazetteer: It contains historical tyrolean names of places (core region: districts Kufstein, Schwaz) within the mining documents Hs. 37 and Hs. 1587 and their modern equivalents enriched with unique Identifiers. Furthermore, Places were localised and georeferenced as far as current scientific data and knowledge permit. A final column shows corresponding IDs between Hs. 37 and Hs. 1587. 2) Historical mine register: The file contains historical Tyrolean names of mines and pits (core region: district Kufsten, Schwaz) within the mining documents Hs. 37 and Hs. 1587 as well as a modern standardisation of the names. The mines also received unique Identifiers. The registers were generated by the research team of the project “Text Mining Medieval Mining Texts” (2019-2022). Two historical mining sources were processed: “Verleihbuch der Rattenberger Bergrichter” ( Hs. 37, 1460-1463) and “Schwazer Berglehenbuch” (Hs. 1587, approx. 1515) stored by the Tyrolean Regional Archive, Innsbruck (Austria). The central research objective of T.M.M.M.T. is the extraction and representation of the legal relationships between people, claims and mines over space and time. Furthermore it deals with the semantically opening and visualisation of the montanistic network of two tyrolean mining regions. Citeable Transcripts are online available:<br> Hs. 37 DOI: 10.5281/zenodo.6274562<br> Hs. 1587 DOI: 10.5281/zenodo.6274928 The research project (2019-2022) was carried out at the university of Innsbruck and funded by go!digital next generation programme of the Austrian Academy of Sciences.
DOI: 10.5281/zenodo.6276619
title: ENHG Annotation of "Verleihbuch der Rattenberger Bergrichter" (TLA Hs. 37, 1460-1463)

description: The dataset contains the TEI tags of the historical mining document "Verleihbuch der Rattenberger Bergrichter" (TLA Hs. 37, 1460-1463) that is currently stored in the Tyrolean Regional archives (Innsbruck). The citeable Transcript is online available (DOI: 10.10.5281/zenodo.6274928) as well as the related annotation guidelines (DOI: 10.5281/zenodo.6275197) The data was generated by the research team of the project “Text Mining Medieval Mining Texts” (T.M.M.M.T.). The research project (2019-2022) was carried out at the University of Innsbruck and funded by go!digital next generation programme of the Austrian Academy of Sciences.
DOI: 10.5281/zenodo.6276521
title: Historical Person Register, Tyrol 15th, 16th century

description: The dataset consists of a file in CSV format (UTF 8). The historical person register contains late medieval / ENHG person names within the Tyrolean mining documents Hs. 37 and Hs. 1587 (15th and 16th century) as well as a modern standardisation of the names. The persons received unique Identifiers. The names are also split into title, first name, last name, occupation and descriptor. Furthermore, the year is added, provenance if available, and alternate writings of the names within the text. The project “Text Mining Medieval Mining Texts” (2019-2022) processed two historical mining sources: “Verleihbuch der Rattenberger Bergrichter” ( Hs. 37, 1460-1463) and “Schwazer Berglehenbuch” (Hs. 1587, approx. 1515) stored by the Tyrolean Regional Archive, Innsbruck (Austria). The central research objective of T.M.M.M.T. is the extraction and representation of the legal relationships between people, claims and mines over space and time. Furthermore it deals with the semantically opening and visualisation of the montanistic network of two tyrolean mining regions. Citeable Transcripts are online available:<br> Hs. 37 DOI: 10.5281/zenodo.6274562<br> Hs. 1587 DOI: 10.5281/zenodo.6274928 The research project (2019-2022) was carried out at the university of Innsbruck and funded by go!digital next generation programme of the Austrian Academy of Sciences.
DOI: 10.6084/m9.figshare.19382993
title: Part of the experimental dataset

description: We uploaded some images of cub-200-20011 and Stanford dogs datasets
DOI: 10.6084/m9.figshare.19382993.v1
title: Part of the experimental dataset

description: We uploaded some images of cub-200-20011 and Stanford dogs datasets
DOI: 10.25919/ks91-yy26
title: Parkes observations for project P1041 semester 2021OCTS_05

description: We propose to carry out a long-term timing campaign of millisecond pulsars (MSPs) discovered in the Globular Cluster Omega Centauri. So far, we have discovered seven MSPs and obtained coherent timing solutions for five of them. Monthly observations with the Parkes Ultra-Wideband Low (UWL) receiver system are requested to time known pulsars and search for new pulsars. Long-term timing and polarisation observations of these MSPs will provide us a new way to (1) study the dynamics of the core and constrain the existence of any intermediate-mass black holes; (2) reveal the origin of Gamma-ray emission from the core and put stringent constraints on dark matter parameters; (3) study Galactic ionised medium and magnetic fields on parsec scales.
DOI: 10.48370/ofd/hepazq
title: Bitoma crenata (Fabricius, 1775), C-557

description: Coleoptera collection
DOI: 10.48370/ofd/lqbaqb
title: Bitoma crenata (Fabricius, 1775), C-558

description: Coleoptera collection
DOI: 10.6084/m9.figshare.16802671
title: Measurements of air-broadening parameters of water vapour transitions in the 7520–8586 cm<sup>−1</sup> spectral region

description: The water vapour line broadening (<i>γ</i>) and shift (<i>δ</i>) coefficients for 319 lines of 11 vibrational bands 2ν<sub>3</sub>, 2ν<sub>2</sub> +ν<sub>3</sub>, 3ν<sub>2</sub>+ν<sub>3</sub>, 5ν<sub>2</sub>, 6ν<sub>2</sub>, ν<sub>1</sub>+ν<sub>3</sub>, ν<sub>1</sub>+ν<sub>2</sub>+ν<sub>3</sub>, ν<sub>1</sub>+2ν<sub>2</sub>, ν<sub>1</sub>+3ν<sub>2</sub>, 2ν<sub>1</sub>, and 2ν<sub>1</sub>+ν<sub>2</sub> from the spectral region 7520–8586 cm<sup>–1</sup> induced by air pressure were measured with a Bruker IFS 125 spectrometer. The measurements were performed at a spectral resolution of 0.01 cm<sup>–1</sup> and over a wide air pressure range. The calculations of the line-shift coefficients <i>δ</i> were performed in the framework of a semi-classical method. The measured air-broadening coefficients <i>γ</i> were combined with literature data for air- and N<sub>2</sub>-broadening coefficients <i>γ</i> measured for the lines in the 380–22,593 cm<sup>–1</sup> spectral region. Combined experimental data for <i>N </i>= 4110 measured coefficients <i>γ</i> of 76 vibrational bands of H<sub>2</sub>O molecules were fitted to the empirical functions. The computed coefficients <i>γ</i> derived from the fitted parameters were statistically compared with the measurements. The vibrational dependence of the measured <i>γ</i> values and their compatibility is discussed.
DOI: 10.6084/m9.figshare.16802671.v1
title: Measurements of air-broadening parameters of water vapour transitions in the 7520–8586 cm<sup>−1</sup> spectral region

description: The water vapour line broadening (<i>γ</i>) and shift (<i>δ</i>) coefficients for 319 lines of 11 vibrational bands 2ν<sub>3</sub>, 2ν<sub>2</sub> +ν<sub>3</sub>, 3ν<sub>2</sub>+ν<sub>3</sub>, 5ν<sub>2</sub>, 6ν<sub>2</sub>, ν<sub>1</sub>+ν<sub>3</sub>, ν<sub>1</sub>+ν<sub>2</sub>+ν<sub>3</sub>, ν<sub>1</sub>+2ν<sub>2</sub>, ν<sub>1</sub>+3ν<sub>2</sub>, 2ν<sub>1</sub>, and 2ν<sub>1</sub>+ν<sub>2</sub> from the spectral region 7520–8586 cm<sup>–1</sup> induced by air pressure were measured with a Bruker IFS 125 spectrometer. The measurements were performed at a spectral resolution of 0.01 cm<sup>–1</sup> and over a wide air pressure range. The calculations of the line-shift coefficients <i>δ</i> were performed in the framework of a semi-classical method. The measured air-broadening coefficients <i>γ</i> were combined with literature data for air- and N<sub>2</sub>-broadening coefficients <i>γ</i> measured for the lines in the 380–22,593 cm<sup>–1</sup> spectral region. Combined experimental data for <i>N </i>= 4110 measured coefficients <i>γ</i> of 76 vibrational bands of H<sub>2</sub>O molecules were fitted to the empirical functions. The computed coefficients <i>γ</i> derived from the fitted parameters were statistically compared with the measurements. The vibrational dependence of the measured <i>γ</i> values and their compatibility is discussed.
DOI: 10.6084/m9.figshare.14672085
title: mdd2018

description: GWAS summary statistics from the Psychiatric Genomics Consortium (PGC)
DOI: 10.6084/m9.figshare.14672085.v1
title: mdd2018

description: GWAS summary statistics from the Psychiatric Genomics Consortium (PGC)
DOI: 10.5281/zenodo.4892780
title: Density, kinematic viscosity, surface tension, distillation curve, and flash point data of diesel-biodiesel blends

description: This dataset contains measurement data for density, kinematic viscosity, surface tension, distillation curve, initial boiling point, and flash point for different biodiesel samples blended with standard diesel fuel. Properties of coconut oil methyl ester, palm oil methyl ester, and biodiesel from waste cooking oil were evaluated in terms of temperature and biodiesel volume fraction. For a detailed description of the measurements and further information, please see the published paper.
DOI: 10.48370/ofd/hvtvcq/smgmij
title: Synchita humeralis.jpg

description: :unav
DOI: 10.48370/ofd/hvtvcq
title: Synchita humeralis (Fabricius, 1792), C-559

description: Coleoptera collection
DOI: 10.5281/zenodo.6364808
title: Microscopi: extended data

description: Microscopi: extended data
DOI: 10.5281/zenodo.4568757
title: Microscopi: extended data

description: Microscopi: extended data
DOI: 10.48370/ofd/pn3gzd/byrfql
title: Synchita humeralis.jpg

description: :unav
DOI: 10.48370/ofd/pn3gzd
title: Synchita humeralis (Fabricius, 1792), C-560

description: Coleoptera collection
DOI: 10.48370/ofd/jhp3hj/wlo29s
title: Pycnomerus terebrans.jpg

description: :unav
DOI: 10.48370/ofd/jhp3hj
title: Pycnomerus terebrans (Olivier, 1790), C-561

description: Coleoptera collection
DOI: 10.4228/zalf.6x5w-yc93
title: Pollinator guilds respond contrastingly at different scales to landscape parameters of land-use intensity (Part 1 of data collection, table data_coordinates)

description: Wild bee and hoverly species and abundance caught with pan traps (yellow, blue, white) on dry grasslands (n=22) in the AgroScapeLab in three sampling campaigns (May, June, August). Additionally, flowering forb species in the vicinity of traps (r=5m) were recorded. Research domain: Ecology of Agricultural Landscapes Data Collection: Related tables see under Related Identifier
DOI: 10.5519/qd.6sonsou4
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (9 records)
DOI: 10.5517/ccdc.csd.cc1zsl4h
title: CCDC 1840877: Experimental Crystal Structure Determination

description: Related Article: Jin‐Miao Wang, Ya‐Rong Liu, Xue‐Yang Mao, Ning‐Ning Shi, Xia Zhang, Hui‐Sheng Wang, Yu‐Hua Fan, Mei Wang|2019|Chem.Asian J.|14|2685|doi:10.1002/asia.201900531
DOI: 10.5517/ccdc.csd.cc1zsl5j
title: CCDC 1840878: Experimental Crystal Structure Determination

description: Related Article: Jin‐Miao Wang, Ya‐Rong Liu, Xue‐Yang Mao, Ning‐Ning Shi, Xia Zhang, Hui‐Sheng Wang, Yu‐Hua Fan, Mei Wang|2019|Chem.Asian J.|14|2685|doi:10.1002/asia.201900531
DOI: 10.5517/ccdc.csd.cc20y2vx
title: CCDC 1874999: Experimental Crystal Structure Determination

description: Related Article: Jayeeta Bhattacharjee, Adimulam Harinath, Alok Sarkar, Tarun K. Panda|2019|ChemCatChem|11|3366|doi:10.1002/cctc.201900920
DOI: 10.5517/ccdc.csd.cc21nm9n
title: CCDC 1896651: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmbp
title: CCDC 1896652: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmcq
title: CCDC 1896653: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmdr
title: CCDC 1896654: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmfs
title: CCDC 1896655: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmgt
title: CCDC 1896656: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmhv
title: CCDC 1896657: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc21nmjw
title: CCDC 1896658: Experimental Crystal Structure Determination

description: Related Article: Benjamin Herzigkeit, Ramona Jurgeleit, Benedikt M. Flöser, Nadja E. Meißner, Tobias A. Engesser, Christian Näther, Felix Tuczek|2019|Eur.J.Inorg.Chem.|2019|2258|doi:10.1002/ejic.201900242
DOI: 10.5517/ccdc.csd.cc220gp8
title: CCDC 1907079: Experimental Crystal Structure Determination

description: Related Article: Ved P. Singh, Jayanta Dowarah, Lalhruaizela, David K. Geiger|2020|Cryst.Res.Technol.|55|1900136|doi:10.1002/crat.201900136
DOI: 10.5517/ccdc.csd.cc24qxb4
title: CCDC 1988226: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxc5
title: CCDC 1988227: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxd6
title: CCDC 1988228: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxf7
title: CCDC 1988229: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxg8
title: CCDC 1988230: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxh9
title: CCDC 1988231: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxjb
title: CCDC 1988232: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxkc
title: CCDC 1988233: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxld
title: CCDC 1988234: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc24qxmf
title: CCDC 1988235: Experimental Crystal Structure Determination

description: Related Article: Mariam A. Al‐Azzani, Faizah Al‐Mjeni, Ryoji Mitsuhashi, Masahiro Mikuriya, Imaddin A. Al‐Omari, Craig C. Robertson, Eckhard Bill, Musa S. Shongwe|2020|Chem.-Eur.J.|26|4766|doi:10.1002/chem.201904744
DOI: 10.5517/ccdc.csd.cc250rcb
title: CCDC 1996721: Experimental Crystal Structure Determination

description: Related Article: Jayanta Dowarah, Brilliant N. Marak, Lalhruaizela, Balkaran S. Sran, Ved P. Singh|2020|Cryst.Res.Technol.|55|2000100|doi:10.1002/crat.202000100
DOI: 10.25625/kbe5or/xkc3yx
title: 1.pdf

description: :unav
DOI: 10.25625/kbe5or
title: Annette Strauch-Davey (2022). Newsletter fdm_ubhildesheim - Forschungsdatenmanagement. 03.01.2022

description: fdm_ubhildesheim Forschungsdatenmanagement
DOI: 10.34740/kaggle/dsv/3319138
title: YouTube Trending Video Dataset (updated daily)
DOI: 10.5519/qd.naxv1zrj
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (241 records)
DOI: 10.5519/qd.gfjj68ch
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (263 records)
DOI: 10.25625/jxfope/d4inub
title: 2.pdf

description: :unav
DOI: 10.25625/jxfope
title: Strauch-Davey, Annette, 2022, Annette Strauch-Davey (2022). Newsletter fdm_ubhildesheim - Forschungsdatenmanagement. 25.11.2021

description: Newsletter
DOI: 10.48370/ofd/wxldxu/etq8wi
title: Trixagus carinifrons.jpg

description: :unav
DOI: 10.48370/ofd/wxldxu
title: Trixagus carinifrons (Bonvouloir, 1859), C-562

description: Coleoptera collection
DOI: 10.34740/kaggle/dsv/3319157
title: 2021 World Population (updated daily)
DOI: 10.6084/m9.figshare.19383236
title: Supplementary Material for: Impact of Human Papillomavirus on Wnt/Beta-Catenin Signaling in Morphological Inconspicuous Cervicovaginal Cells

description: <b><i>Introduction:</i></b> The aim of this study was to identify early changes in the Wnt/beta-catenin signaling pathway in high-risk human papillomavirus (HPV) infected cervicovaginal cells and to correlate these changes with cell proliferation, apoptosis, and autophagic processes. <b><i>Methods:</i></b> We evaluated 91 cervicovaginal smears of women with (<i>n</i> = 41) and without (<i>n</i> = 50) HPV-DNA. Smears were stained against beta-catenin, c-myc, secreted frizzled-related protein 4 (sFRP4), cleaved caspase-3, and the autophagy markers Beclin-1 and light chain 3B. In addition, sFRP-1, -2, -3, -4, -5 mRNA levels were determined by quantitative reverse transcription-PCR in primary keratinocytes and FaDu cells expressing HPV16-E6, -E7, or -E6E7. <b><i>Results:</i></b> Our data indicated that the Wnt/beta-catenin signaling is activated in HPV (+) cervicovaginal cells that can already be detected in cells with no obvious changes in cellular morphology (HPV [+]/cyto [−]). These cells also had significantly higher sFRP4 levels when compared to HPV-negative samples. In primary keratinocytes, sFRP4 was found to be absent and sFRP1 and sFRP2 to be repressed in the presence of HPV16-E6 and E7. Interestingly, sFRP4 is expressed in FaDu cells and can be upregulated in the presence of E6E7. Curiously, SFRP4 expression correlated with an increase in the level of autophagic markers in HPV (+)/cyto (−) smears. <b><i>Conclusion:</i></b> In conclusion, the activation of the Wnt/beta-catenin signaling pathway and upregulation of sFRP4, paralleled by an activation of the autophagic pathway may represent predisposing cellular factors early after HPV infection which need to be further determined in larger study.
DOI: 10.6084/m9.figshare.19383236.v1
title: Supplementary Material for: Impact of Human Papillomavirus on Wnt/Beta-Catenin Signaling in Morphological Inconspicuous Cervicovaginal Cells

description: <b><i>Introduction:</i></b> The aim of this study was to identify early changes in the Wnt/beta-catenin signaling pathway in high-risk human papillomavirus (HPV) infected cervicovaginal cells and to correlate these changes with cell proliferation, apoptosis, and autophagic processes. <b><i>Methods:</i></b> We evaluated 91 cervicovaginal smears of women with (<i>n</i> = 41) and without (<i>n</i> = 50) HPV-DNA. Smears were stained against beta-catenin, c-myc, secreted frizzled-related protein 4 (sFRP4), cleaved caspase-3, and the autophagy markers Beclin-1 and light chain 3B. In addition, sFRP-1, -2, -3, -4, -5 mRNA levels were determined by quantitative reverse transcription-PCR in primary keratinocytes and FaDu cells expressing HPV16-E6, -E7, or -E6E7. <b><i>Results:</i></b> Our data indicated that the Wnt/beta-catenin signaling is activated in HPV (+) cervicovaginal cells that can already be detected in cells with no obvious changes in cellular morphology (HPV [+]/cyto [−]). These cells also had significantly higher sFRP4 levels when compared to HPV-negative samples. In primary keratinocytes, sFRP4 was found to be absent and sFRP1 and sFRP2 to be repressed in the presence of HPV16-E6 and E7. Interestingly, sFRP4 is expressed in FaDu cells and can be upregulated in the presence of E6E7. Curiously, SFRP4 expression correlated with an increase in the level of autophagic markers in HPV (+)/cyto (−) smears. <b><i>Conclusion:</i></b> In conclusion, the activation of the Wnt/beta-catenin signaling pathway and upregulation of sFRP4, paralleled by an activation of the autophagic pathway may represent predisposing cellular factors early after HPV infection which need to be further determined in larger study.