cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/qav5a-ddh81
title: Vibrio cholerae C6706 ∆lacZ

description: Wild Type Vibrio
description: <strong>Tilt Series Date:</strong> 2015-06-02</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Simon Ringgaard</p>
  aba2015-06-02-29_slicer13884.jpg:  .  [2015-11-21 00:35:21] hollow spheres at the bottom vaguely resemble structures seen in yc2015-06-17-31 and ab2015-06-02-29.  In this picture, they appear to be membrane invaginations.-andrew
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-06-02-29/rawdata/</p>VibrioLacZ0001.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-06-02-29/rawdata/VibrioLacZ0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioLacZ0001_part121_5.rec,  Reconstruction (Pixel Size 1.28 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-06-02-29/3dimage_57904/VibrioLacZ0001_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>

        aba2015-06-02-29_slicer16424.png,  These hollow spheres resemble structures seen in yc2015-06-17-31 and ab2015-06-02-29.  However these spheres are a little bit bigger than those (25nm-44nm).  These may be invaginations of the inner membrane. .  -andrew, 165.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/aba2015-06-02-29_slicer16424.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m94zc-7xn74
title: HeLa cells expressing TRIM5α-YFP

description: HeLa cell line 25 expressing stable TRIM5α-YFP. Correlated lightelectron microscopy (11062015Tom). Concentration of 500 nmblue beads (phosphorex) 4/150. 20 nm gold highly concentrated.Manual blotting with Whatman 40.HeLa cell line 25 expressingstable TRIM5α-YFP.
description: <strong>Tilt Series Date:</strong> 2015-10-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cell line 25 and VSV-G pseudotyped virus Vpr-Tomato provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells infected with pseudovirus Vpr-Tomato (in box). 500 µl of media and 500 µl of virus. Incubated with MG-132 (Campbell et al 2008) 6hrs. Cells were washed with warm media and incubated at 37°C before freezing.</p>
<strong>Sample Preparation:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-7/3dimage_71016/</p>tomography18.rec,  Reconstruction (Pixel Size 24.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-7/3dimage_71016/tomography18.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography18.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-7/rawdata/tomography18.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography18_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-7/3dimage_76108/tomography18_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h9zy2-62f59
title: V.cholerae

description: This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.
description: <strong>Tilt Series Date:</strong> 2017-01-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Yi-wei</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: -60, 60.         dosage: 160e/A2. defocus: -6um. magnification: 27500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-25/3dimage_80122/</p>atlas50003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-25/3dimage_80122/atlas50003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas50003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-25/rawdata/atlas50003.mrc"
        > <i class="download icon"></i> Download </a></p>

        psa2017-01-06-25_slicer19185.png,  large REZ .  -andrew, 875.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2017-01-06-25_slicer19185.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y1f6v-jwn60
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-29/rawdata/</p>20160424_YWC212_40002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-29/rawdata/20160424_YWC212_40002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_40002_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-29/3dimage_74821/20160424_YWC212_40002_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_40002_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-29/3dimage_74822/20160424_YWC212_40002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3h706-y3910
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-11-08</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-57.0°, 57.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-60/rawdata/</p>Fjoh4_27.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-60/rawdata/Fjoh4_27.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/95z5t-bk510
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-11-08</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-57.0°, 57.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-107/rawdata/</p>Fjoh4_74.mrc,  Tilt Series (Pixel Size 0.39 nm), 113.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-11-08-107/rawdata/Fjoh4_74.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16951152
title: Code and Data from Pinho et al. - "Fragmented tropical forests can maintain tree diversity and function, but not under high deforestation"

description: This repository contains code and data used in the analyses for the manuscript entitled: 

"Fragmented tropical forests can maintain tree diversity and function, but not under high deforestation" - Pinho et al. 2025, under review in Ecology Letters.

See README.txt for details on the available materials.
DOI: 10.22002/y91z4-gf194
title: Unspecified

description: Raw data files of Unspecified
description: <strong>Tilt Series Date:</strong> 2018-10-22</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-82/rawdata/</p>Fjoh1_42.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2018-10-22-82/rawdata/Fjoh1_42.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sfqza-new63
title: Ecoli_EPEC_8612

description: These are E coli cells (EPEC 8612) obtained from Ritesh (Ilan Rosenshine lab at the Hebrew  .  university). The idea is to image bacterial nanotubes in these cells.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These cells were prepared with Ritesh in Ilan Rosenshine lab at the Hebrew University. </p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. Then washed with PBS and blotted and then plunge . frozen. Yuxi helped in sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-42/rawdata/</p>EPEC_8612_tilt15.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-42/rawdata/EPEC_8612_tilt15.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt15_part35_2.rec,  Reconstruction (Pixel Size 1.796 nm), 501.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-42/3dimage_110702/EPEC_8612_tilt15_part35_2.rec"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt15.mrc.mdoc,  19.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-42/rawdata/EPEC_8612_tilt15.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rngtq-nf950
title: BacillusSubtilis_328_4hr

description: This is Bacillus Subtilis tomograms PonA mutant strain 328 (obtained from Amit in Sigal Ben  .  Yehuda lab at the Hebrew university). The idea is to image bacterial nanotubes in Bacilus .  Subtilis.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB328, ΔponA ΔymdB amyE::ymdB Δhag</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates . (I will write the details on my Labjournal).  </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were grown on the grids on agar plates for four hours. Detailed description is in the lab . journal. Yuxi helped in the sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> Samples were taken from the agar plates and washed few times in PBS. Then they were blotted . and plunge-frozen.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-4/rawdata/</p>Bsubtilis_328_4hr_conventional4_Positive.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-4/rawdata/Bsubtilis_328_4hr_conventional4_Positive.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_328_4hr_conventional4_Positive_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-4/3dimage_110650/Bsubtilis_328_4hr_conventional4_Positive_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_328_4hr_conventional4_Positive.mrc.mdoc,  13.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-4/rawdata/Bsubtilis_328_4hr_conventional4_Positive.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/519ff-xpg11
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-120/rawdata/</p>Ecoli_CONjugation8.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-120/rawdata/Ecoli_CONjugation8.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation8_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-120/3dimage_107212/Ecoli_CONjugation8_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation8.mrc.mdoc,  24.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-120/rawdata/Ecoli_CONjugation8.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xfff4-s6q52
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-50/rawdata/</p>Ecoli_CONjugation21.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-50/rawdata/Ecoli_CONjugation21.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation21_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-50/3dimage_107108/Ecoli_CONjugation21_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation21.mrc.mdoc,  50.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-50/rawdata/Ecoli_CONjugation21.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>