cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/qyhb9-6ay38
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-3/3dimage_69364/</p>12may29_C135_1_MEFnomitoDNA_AT1BT30004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-3/3dimage_69364/12may29_C135_1_MEFnomitoDNA_AT1BT30004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may29_C135_1_MEFnomitoDNA_AT1BT30004.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-3/rawdata/12may29_C135_1_MEFnomitoDNA_AT1BT30004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ksfm1-z5t20
title: UU 2801- P221D delta P1P2

description: Strain P221D (low kinase activity) with P1P2 deletion in CheA
description: <strong>Tilt Series Date:</strong> 2012-09-18</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase, then lysed for one hour with Penicillin</p>
<strong>Sample Preparation:</strong> cells plunge frozen on Quantifoul R2/2 in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-18-9/rawdata/</p>Coli2801atlas0917_20010.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-18-9/rawdata/Coli2801atlas0917_20010.mrc"
        > <i class="download icon"></i> Download </a></p>

        Coli2801atlas0917_20010_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-18-9/3dimage_20856/Coli2801atlas0917_20010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4s7za-00a77
title: gold labeled CheA- in vitro

description: post wash sample - gold labeled CheA, 1.8 nm gold, post ternary formation snap rxn.
description: <strong>Tilt Series Date:</strong> 2013-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Heather Hodge and Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Buffer: 1x Snap + 1mM DTT 50 mM Tris Hcl pH 7.5, 100 mM Ncl, 0.1% Tween-200)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-60/rawdata/</p>HTH1B_10014.mrc,  Tilt Series (Pixel Size 0.2989 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-60/rawdata/HTH1B_10014.mrc"
        > <i class="download icon"></i> Download </a></p>

        HTH1B_10014_part67_2.rec,  Reconstruction (Pixel Size 0.5978 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-60/3dimage_26507/HTH1B_10014_part67_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gk7b9-7ry09
title: E. coli pED208

description: E. coli with plasmid pED208
description: <strong>Tilt Series Date:</strong> 2014-12-16</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells arrived dead.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-33/rawdata/</p>pED2080025.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-33/rawdata/pED2080025.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080025_part121_4.rec,  Reconstruction (Pixel Size 2.52 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-33/3dimage_48842/pED2080025_part121_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pstnc-9p928
title: HIV capsid purified trim5 alpha

description: 26/A2 slit B grid, 22500 8 defocus  0.3 exposure
description: <strong>Tilt Series Date:</strong> 2013-12-12</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 26.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod and SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified recombinant trim5 alpha decorated HIV cores</p>
<strong>Sample Preparation:</strong> B grid</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-12-13/rawdata/</p>Trim5alpha22500bgrid0013.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-12-13/rawdata/Trim5alpha22500bgrid0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        Trim5alpha22500bgrid0013_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-12-13/3dimage_36014/Trim5alpha22500bgrid0013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/brb8w-d3a05
title: Bsubtilis_ponA_sacculi

description: Bsubtilis_ponA_sacculi_withTA
description: <strong>Tilt Series Date:</strong> 2012-09-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> subsp. subtilis str. 168</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-02-23/rawdata/</p>mj_thin7.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-02-23/rawdata/mj_thin7.mrc"
        > <i class="download icon"></i> Download </a></p>

        mj11_full.rec,  Reconstruction (Pixel Size 2.0 nm), 678.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-02-23/3dimage_57071/mj11_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/avs3h-2sp53
title: Alongum

description: Alongum_periplasmiclayers
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-49/rawdata/</p>alongLys_20030.mrc,  Tilt Series (Pixel Size 0.95 nm), 512.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-49/rawdata/alongLys_20030.mrc"
        > <i class="download icon"></i> Download </a></p>

        alongLys_20030_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-49/3dimage_56818/alongLys_20030_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/spr42-14a95
title: gold labeled CheA- in vitro

description: post wash sample - gold labeled CheA, 1.8 nm gold, post ternary formation snap rxn.
description: <strong>Tilt Series Date:</strong> 2013-03-22</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Heather Hodge and Laura Kiessling</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Buffer: 1x Snap + 1mM DTT 50 mM Tris Hcl pH 7.5, 100 mM Ncl, 0.1% Tween-200)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-59/rawdata/</p>HTH1B_10013.mrc,  Tilt Series (Pixel Size 0.2989 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-59/rawdata/HTH1B_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        HTH1B_10013_part67_2.rec,  Reconstruction (Pixel Size 0.5978 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-03-22-59/3dimage_26501/HTH1B_10013_part67_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.29991799
title: The Core Hypothesis in P vs NP: A Theoretical Framework

description: This paper introduces the Core Hypothesis as a new perspective on the P vs NP problem. The hypothesis suggests that within any computational problem, there exists a core—a minimal, indivisible subset of constraints or conditions—that determines the essential difficulty of the problem. By identifying or approximating this core, one can reduce the search space of candidate solutions and potentially transform exponential complexity into polynomial tractability. Random sampling is proposed as a mechanism to discover the core by iteratively eliminating redundancies and highlighting essential constraints. This framework does not claim to prove P = NP but rather provides a novel theoretical pathway for exploration
DOI: 10.6084/m9.figshare.29991799.v1
title: The Core Hypothesis in P vs NP: A Theoretical Framework

description: This paper introduces the Core Hypothesis as a new perspective on the P vs NP problem. The hypothesis suggests that within any computational problem, there exists a core—a minimal, indivisible subset of constraints or conditions—that determines the essential difficulty of the problem. By identifying or approximating this core, one can reduce the search space of candidate solutions and potentially transform exponential complexity into polynomial tractability. Random sampling is proposed as a mechanism to discover the core by iteratively eliminating redundancies and highlighting essential constraints. This framework does not claim to prove P = NP but rather provides a novel theoretical pathway for exploration
DOI: 10.6084/m9.figshare.29868518.v14
title: Logic and being pdf v1-2-3-4-5-6 NEW

description: Logic and being
DOI: 10.22002/trwjj-gc890
title: Agro C1 induced at 19C

description: Agro C1, no Ti plasmid, induced at 19 in liquid for 24h  AB+AS
description: <strong>Tilt Series Date:</strong> 2014-03-26</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Agro C1, no Ti plasmid, induced at 19 in liquid AB+AS</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-26-8/rawdata/</p>Agro_C10008.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-26-8/rawdata/Agro_C10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        Agro_C10008_part121_3.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-26-8/3dimage_49461/Agro_C10008_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/htkyw-vf050
title: Agrobacterium tumefaciens

description: Agro VG3101
description: <strong>Tilt Series Date:</strong> 2013-10-31</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid LB+antibiotics</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-31-18/rawdata/</p>agro_vg31010005.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-31-18/rawdata/agro_vg31010005.mrc"
        > <i class="download icon"></i> Download </a></p>

        agro_vg31010005_part121_36.rec,  Reconstruction (Pixel Size 0.0 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-31-18/3dimage_49065/agro_vg31010005_part121_36.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yt11h-6j406
title: HIV capsid

description: Box C, 0.5 exposure, 152, 41000 mag 3 defocus, slit 20 eV, search track 13500
description: <strong>Tilt Series Date:</strong> 2013-12-05</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 152.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod, need to reconstruct with SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified HIV cores</p>
<strong>Sample Preparation:</strong> Box C</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-41/rawdata/</p>hivcapsid00008.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-41/rawdata/hivcapsid00008.mrc"
        > <i class="download icon"></i> Download </a></p>

        hivcapsid00008_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-41/3dimage_35572/hivcapsid00008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yetjj-11s76
title: HUVECs infx pNLGFP

description: Raw data files of HUVECs infx pNLGFP
description: <strong>Tilt Series Date:</strong> 2013-11-08</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 24h post infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-08-18/rawdata/</p>13nov08_C176-2_pNLgfpHUVECs_AT3BT1b_0004.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-08-18/rawdata/13nov08_C176-2_pNLgfpHUVECs_AT3BT1b_0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        13nov08_C176-2_pNLgfpHUVECs_AT3BT1b_0004_full.rec,  Reconstruction (Pixel Size 1.96 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-08-18/3dimage_34465/13nov08_C176-2_pNLgfpHUVECs_AT3BT1b_0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1btbs-hw922
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C111
description: <strong>Tilt Series Date:</strong> 2012-02-18</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-7/3dimage_68739/</p>12feb18_C111_pNL_INF_AT1BT10008_part122_10.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-7/3dimage_68739/12feb18_C111_pNL_INF_AT1BT10008_part122_10.rec"
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        12feb18_C111_pNL_INF_AT1BT10008.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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