cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.57760/sciencedb.14839
title: Fractionated radiation alters the extracellular matrix produced by muscle-invasive bladder cancer cells
title: Fractionated radiation alters the extracellular matrix produced by muscle-invasive bladder cancer cells

description: MS analysis was performed following previously described protocols (Humphries et al., 2022; J. Robertson et al., 2015). In short, 5 µg of protein was loaded onto a 4-15% agarose gel (Thermo Fisher Scientific for SDS-PAGE electrophoresis (3 min, 200V; Thermo Fisher Scientific). Protein bands were stained with InstantBlue Coomassie (Abcam), sectioned, and in-gel trypsin digested before being analysed by tandem liquid chromatography-mass spectrometry (LC-MS/MS) using an UltiMate 3000 Rapid Separation LC system (Thermo Fisher Scientific) coupled with an Orbitrap Elite Mass Spectrometer (Thermo Fisher Scientific).Following previously described protocols (Byron et al., 2015; J. Robertson et al., 2015), MS data was analysed using an in-house Mascot Server (v. 2.5.1; Matrix Sciences) with mass tolerances of 0.4 Da and 0.5 Da for precursor and fragment ions, respectively. Data was validated using Scaffold (v. 4.6.3; Thermo Fisher Scientific) with an identification threshold of 90% at the peptide level, with at least one unique validated peptide (0.1% estimated false discovery rate). Protein identification, normalisation, and fold-change and p-values calculations were performed with Protein Discoverer (v. 2.5.0.400; ThermoFisher Scientific).
description: MS analysis was performed following previously described protocols (Humphries et al., 2022; J. Robertson et al., 2015). In short, 5 µg of protein was loaded onto a 4-15% agarose gel (Thermo Fisher Scientific for SDS-PAGE electrophoresis (3 min, 200V; Thermo Fisher Scientific). Protein bands were stained with InstantBlue Coomassie (Abcam), sectioned, and in-gel trypsin digested before being analysed by tandem liquid chromatography-mass spectrometry (LC-MS/MS) using an UltiMate 3000 Rapid Separation LC system (Thermo Fisher Scientific) coupled with an Orbitrap Elite Mass Spectrometer (Thermo Fisher Scientific).Following previously described protocols (Byron et al., 2015; J. Robertson et al., 2015), MS data was analysed using an in-house Mascot Server (v. 2.5.1; Matrix Sciences) with mass tolerances of 0.4 Da and 0.5 Da for precursor and fragment ions, respectively. Data was validated using Scaffold (v. 4.6.3; Thermo Fisher Scientific) with an identification threshold of 90% at the peptide level, with at least one unique validated peptide (0.1% estimated false discovery rate). Protein identification, normalisation, and fold-change and p-values calculations were performed with Protein Discoverer (v. 2.5.0.400; ThermoFisher Scientific).
DOI: 10.57760/sciencedb.j00217.03856
title: Research progress of MicroRNA in allergic diseases
title: MicroRNA在过敏性疾病中的研究进展

description: In recent years, the incidence of allergic diseases has increased year by year, which affects people's social life, study, work and sleep to varying degrees, and also increases the economic burden of families and society. It is imperative to deeply study the pathogenesis of allergic diseases and develop new preparations for the treatment of allergic diseases. MicroRNA (miRNA) is 19-25 nucleotides in length. miRNA is a key post-transcriptional regulator, which controls many cellular pathways and acts as a key regulatory element in various immunological environments, and plays an important role in regulating the development and differentiation of T cells. Recent studies have found that miRNA plays an important role in the pathogenesis of allergic diseases, and specific miRNA is expected to become a diagnostic marker and potential therapeutic target of allergic diseases.
description: In recent years, the incidence of allergic diseases has increased year by year, which affects people's social life, study, work and sleep to varying degrees, and also increases the economic burden of families and society. It is imperative to deeply study the pathogenesis of allergic diseases and develop new preparations for the treatment of allergic diseases. MicroRNA (miRNA) is 19-25 nucleotides in length. miRNA is a key post-transcriptional regulator, which controls many cellular pathways and acts as a key regulatory element in various immunological environments, and plays an important role in regulating the development and differentiation of T cells. Recent studies have found that miRNA plays an important role in the pathogenesis of allergic diseases, and specific miRNA is expected to become a diagnostic marker and potential therapeutic target of allergic diseases.
DOI: 10.57760/sciencedb.14864
title: Research on the influencing factors and interactions of labyrinth–honeycomb seals via orthogonal experiments and multiple linear regression models
title: Research on the influencing factors and interactions of labyrinth–honeycomb seals via orthogonal experiments and multiple linear regression models

description: 为提高压缩机级间迷宫蜂窝密封的密封性能，提出了一种正交实验设计，研究了 9 个主要参数对泄漏特性的影响和交互作用。最后，建立与几何设计变量相关的多因素线性回归模型来预测泄漏量。结果表明，间隙和压力比对密封特性有显著影响;蜂窝深度、芯直径、翅片高度和翅片间距对密封特性有很大影响。前倾角应向左倾斜，以避免腔内有死角。后倾斜角应向右倾斜，这会引导腔涡流撞击尖端喷射流。线性回归模型对泄漏有较好的预测效果，可为迷宫-蜂窝密封的性能分析和结构设计提供技术参考。
description: 为提高压缩机级间迷宫蜂窝密封的密封性能，提出了一种正交实验设计，研究了 9 个主要参数对泄漏特性的影响和交互作用。最后，建立与几何设计变量相关的多因素线性回归模型来预测泄漏量。结果表明，间隙和压力比对密封特性有显著影响;蜂窝深度、芯直径、翅片高度和翅片间距对密封特性有很大影响。前倾角应向左倾斜，以避免腔内有死角。后倾斜角应向右倾斜，这会引导腔涡流撞击尖端喷射流。线性回归模型对泄漏有较好的预测效果，可为迷宫-蜂窝密封的性能分析和结构设计提供技术参考。
DOI: 10.57760/sciencedb.partic.00056
title: Positive and negative effects of graphite flake and monolayer graphene oxide templates on protein crystallization
title: Positive and negative effects of graphite flake and monolayer graphene oxide templates on protein crystallization

description: Heterogeneous template-induced nucleation is a promising way to regulate protein crystallization events and could be employed for purification processes and crystallographic studies. Protein crystallization process with graphite and graphene oxide (as heterogeneous templates) were investigated. More than 640 hanging drops with different concentrations of Lysozyme (30, 50, 70, 100 mg/mL) and NaCl (0.7, 0.9, 1.1, 1.3, 1.5 M) were crystallised at 4 °C with or without graphite / graphene oxide templates. The induction times and crystallization process were observed under the microscope. The lysozyme in the solutions with graphite flakes nucleated faster under all the conditions than the lysozyme with equal experimental conditions without templates. The crystals preferred to grow around the edge of graphite flakes than on the flat surfaces. In the droplets with monolayer graphene oxide, more crystals appeared around graphene oxide particles, and the faster or slower nucleation process with templates was dependent on the lysozyme and NaCl concentrations. Graphene oxide templates strongly inhibited nucleation at high lysozyme concentrations but promoted nucleation at low lysozyme concentrations. Both heterogeneous templates changed the crystal morphology and the crystallization kinetics. More crystals were observed in the solution with graphite templatesthan with graphene oxide templates and without any template. 
description: Heterogeneous template-induced nucleation is a promising way to regulate protein crystallization events and could be employed for purification processes and crystallographic studies. Protein crystallization process with graphite and graphene oxide (as heterogeneous templates) were investigated. More than 640 hanging drops with different concentrations of Lysozyme (30, 50, 70, 100 mg/mL) and NaCl (0.7, 0.9, 1.1, 1.3, 1.5 M) were crystallised at 4 °C with or without graphite / graphene oxide templates. The induction times and crystallization process were observed under the microscope. The lysozyme in the solutions with graphite flakes nucleated faster under all the conditions than the lysozyme with equal experimental conditions without templates. The crystals preferred to grow around the edge of graphite flakes than on the flat surfaces. In the droplets with monolayer graphene oxide, more crystals appeared around graphene oxide particles, and the faster or slower nucleation process with templates was dependent on the lysozyme and NaCl concentrations. Graphene oxide templates strongly inhibited nucleation at high lysozyme concentrations but promoted nucleation at low lysozyme concentrations. Both heterogeneous templates changed the crystal morphology and the crystallization kinetics. More crystals were observed in the solution with graphite templatesthan with graphene oxide templates and without any template.
DOI: 10.57760/sciencedb.14871
title: Data of belief-fixation model testing
title: Data of belief-fixation model testing

description: Three experiments were conducted to assess participants’ recognition memory for true, false, and uncertain statements (i.e., statements with ambiguous veracity), utilizing a narrative-based false information paradigm.
description: Three experiments were conducted to assess participants’ recognition memory for true, false, and uncertain statements (i.e., statements with ambiguous veracity), utilizing a narrative-based false information paradigm.
DOI: 10.57760/sciencedb.partic.00057
title: A new track for antibacterial treatment: Progress and challenges of using cytomembrane-based vesicles to combat bacteria
title: A new track for antibacterial treatment: Progress and challenges of using cytomembrane-based vesicles to combat bacteria

description: The emergence and re-emergence of antibiotic-resistant bacteria, especially superbugs, are leading to complicated infections that are increasingly difficult to treat. Therefore, novel alternative antimicrobial therapies are urgently needed to reduce the morbidity and mortality caused by antibiotic resistance. The development of biomimetic-based therapy is expected to provide innovative means for addressing this challenging task. As a kind of novel biomaterial, cytomembrane-based vesicles (MVs) continue to receive considerable attention in antimicrobial therapy owing to their inherent biocompatibility, design flexibility, and remarkable ability to interact with biological molecules or the surrounding environment. These remarkable cell-like properties and their inherent interaction with pathogens, toxins, and the immune system underlie MVs-based functional protein therapy and targeted delivery to develop advanced therapeutic strategies against bacterial infection. This review provides a fundamental understanding of the characteristics and physiological functions of cytomembrane-based vesicles, focusing on their potential to combat bacterial infections, including detoxification, immune modulation, antibiotics delivery, and physical therapy. In addition, the future possibilities and remaining challenges for clinically implementing MVs in the field of antibacterial treatment are discussed.
description: The emergence and re-emergence of antibiotic-resistant bacteria, especially superbugs, are leading to complicated infections that are increasingly difficult to treat. Therefore, novel alternative antimicrobial therapies are urgently needed to reduce the morbidity and mortality caused by antibiotic resistance. The development of biomimetic-based therapy is expected to provide innovative means for addressing this challenging task. As a kind of novel biomaterial, cytomembrane-based vesicles (MVs) continue to receive considerable attention in antimicrobial therapy owing to their inherent biocompatibility, design flexibility, and remarkable ability to interact with biological molecules or the surrounding environment. These remarkable cell-like properties and their inherent interaction with pathogens, toxins, and the immune system underlie MVs-based functional protein therapy and targeted delivery to develop advanced therapeutic strategies against bacterial infection. This review provides a fundamental understanding of the characteristics and physiological functions of cytomembrane-based vesicles, focusing on their potential to combat bacterial infections, including detoxification, immune modulation, antibiotics delivery, and physical therapy. In addition, the future possibilities and remaining challenges for clinically implementing MVs in the field of antibacterial treatment are discussed.
DOI: 10.57760/sciencedb.partic.00058
title: Poly(N-isopropylmethacrylamide-co-4-acrylamidobenzo-18-crown-6) microgels with expanded networks for excellent adsorption of lead(II) ions
title: Poly(N-isopropylmethacrylamide-co-4-acrylamidobenzo-18-crown-6) microgels with expanded networks for excellent adsorption of lead(II) ions

description: Lead(II) (Pb2+) ions are toxic heavy metal ions that can accumulate in the human body through water and cause severe health problems, including neurotoxicity, nephrotoxicity, hematological toxicity and even genotoxicity effects. To remove Pb2+ selectively and effectively from aqueous solutions, we develop a novel type of Pb2+-recognizable microgels with excellent adsorption capacity to Pb2+, which are fabricated from 4-acrylamidobenzo-18-crown-6 (B18C6Am) and N-isopropylmethacrylamide (NIPMAM) monomers by precipitation copolymerization method. The prepared poly(N-isopropylmethacrylamide-co-4-acrylamidobenzo-18-crown-6) (PNMB) microgels exhibit expanded structures, because the electron-donating methyl groups at α-carbon could descend the polarity of C=O in the NIPMAM monomers and thus weaken the polymer segment···segment interactions. The expanded structures of PNMB microgels are beneficial for adsorption of Pb2+ due to the low steric hindrance in the polymeric networks. The Pb2+ adsorption isotherms of PNMB microgels are consistent with the Langmuir model for monolayer adsorption. The results indicate that the prepared Pb2+-recognizable PNMB microgels are highly promising for the selective removal of lead(II) ions from aqueous solutions.
description: Lead(II) (Pb2+) ions are toxic heavy metal ions that can accumulate in the human body through water and cause severe health problems, including neurotoxicity, nephrotoxicity, hematological toxicity and even genotoxicity effects. To remove Pb2+ selectively and effectively from aqueous solutions, we develop a novel type of Pb2+-recognizable microgels with excellent adsorption capacity to Pb2+, which are fabricated from 4-acrylamidobenzo-18-crown-6 (B18C6Am) and N-isopropylmethacrylamide (NIPMAM) monomers by precipitation copolymerization method. The prepared poly(N-isopropylmethacrylamide-co-4-acrylamidobenzo-18-crown-6) (PNMB) microgels exhibit expanded structures, because the electron-donating methyl groups at α-carbon could descend the polarity of C=O in the NIPMAM monomers and thus weaken the polymer segment···segment interactions. The expanded structures of PNMB microgels are beneficial for adsorption of Pb2+ due to the low steric hindrance in the polymeric networks. The Pb2+ adsorption isotherms of PNMB microgels are consistent with the Langmuir model for monolayer adsorption. The results indicate that the prepared Pb2+-recognizable PNMB microgels are highly promising for the selective removal of lead(II) ions from aqueous solutions.
DOI: 10.57760/sciencedb.partic.00059
title: High-throughput generation of uniform droplets from parallel microchannel droplet generators and the preparation of polystyrene microsphere material
title: High-throughput generation of uniform droplets from parallel microchannel droplet generators and the preparation of polystyrene microsphere material

description: To prepare uniform polystyrene particles with ten microns of diameter, a parallel scaling-up strategy for the capillary-assembled stepwise microchannel was developed, which created uniform droplets with high-throughput and formed a large amount of emulsion templates for the polymerizations of styrene and cross-linker. The microchannel droplet generator was robust for the flow rate deviation of the continuous phase in the jetting flow, and droplet generation frequency up to 2.8×104 Hz was achieved with only four parallel droplet generators, which were much more efficient than the parallelly scaled microfluidic devices working in dripping flow. 32–52 μm average diameter droplets with 4.5%–8.4% diameter variation coefficients were successfully prepared from the microfluidic device fabricated by low-cost 3D-print method, and the droplets were subsequently turned to solid particles via a two-step polymerization in the microfluidic platform. The polystyrene particles were further reduced to 16.9–23.5 μm with 5.0%–8.6% diameter variation coefficients due to the accompanying emulsion polymerization, and the working capacity of the microfluidic platform reached hundred milligrams of particles per hour.
description: To prepare uniform polystyrene particles with ten microns of diameter, a parallel scaling-up strategy for the capillary-assembled stepwise microchannel was developed, which created uniform droplets with high-throughput and formed a large amount of emulsion templates for the polymerizations of styrene and cross-linker. The microchannel droplet generator was robust for the flow rate deviation of the continuous phase in the jetting flow, and droplet generation frequency up to 2.8×104 Hz was achieved with only four parallel droplet generators, which were much more efficient than the parallelly scaled microfluidic devices working in dripping flow. 32–52 μm average diameter droplets with 4.5%–8.4% diameter variation coefficients were successfully prepared from the microfluidic device fabricated by low-cost 3D-print method, and the droplets were subsequently turned to solid particles via a two-step polymerization in the microfluidic platform. The polystyrene particles were further reduced to 16.9–23.5 μm with 5.0%–8.6% diameter variation coefficients due to the accompanying emulsion polymerization, and the working capacity of the microfluidic platform reached hundred milligrams of particles per hour.
DOI: 10.57760/sciencedb.partic.00060
title: Composing atomic transition metal sites for high-performance bifunctional oxygen electrocatalysis in rechargeable zinc–air batteries
title: Composing atomic transition metal sites for high-performance bifunctional oxygen electrocatalysis in rechargeable zinc–air batteries

description: Rechargeable zinc–air batteries have attracted extensive attention as clean, safe, and high-efficient energy storage devices. However, the oxygen redox reactions at cathode are highly sluggish in kinetics and severely limit the actual battery performance. Atomic transition metal sites demonstrate high electrocatalytic activity towards respective oxygen reduction and evolution, while high bifunctional electrocatalytic activity is seldomly achieved. Herein a strategy of composing atomic transition metal sites is proposed to fabricate high active bifunctional oxygen electrocatalysts and high-performance rechargeable zinc–air batteries. Concretely, atomic Fe and Ni sites are composed based on their respective high electrocatalytic activity on oxygen reduction and evolution. The composite electrocatalyst demonstrates high bifunctional electrocatalytic activity (ΔE = 0.72 V) and exceeds noble-metal-based Pt/C+Ir/C (ΔE = 0.79 V). Accordingly, rechargeable zinc–air batteries with the composite electrocatalyst realize over 100 stable cycles at 25 mA cm−2. This work affords an effective strategy to fabricate bifunctional oxygen electrocatalysts for high-performance rechargeable zinc–air batteries.
description: Rechargeable zinc–air batteries have attracted extensive attention as clean, safe, and high-efficient energy storage devices. However, the oxygen redox reactions at cathode are highly sluggish in kinetics and severely limit the actual battery performance. Atomic transition metal sites demonstrate high electrocatalytic activity towards respective oxygen reduction and evolution, while high bifunctional electrocatalytic activity is seldomly achieved. Herein a strategy of composing atomic transition metal sites is proposed to fabricate high active bifunctional oxygen electrocatalysts and high-performance rechargeable zinc–air batteries. Concretely, atomic Fe and Ni sites are composed based on their respective high electrocatalytic activity on oxygen reduction and evolution. The composite electrocatalyst demonstrates high bifunctional electrocatalytic activity (ΔE = 0.72 V) and exceeds noble-metal-based Pt/C+Ir/C (ΔE = 0.79 V). Accordingly, rechargeable zinc–air batteries with the composite electrocatalyst realize over 100 stable cycles at 25 mA cm−2. This work affords an effective strategy to fabricate bifunctional oxygen electrocatalysts for high-performance rechargeable zinc–air batteries.
DOI: 10.57760/sciencedb.14880
title: Improvements of microstructure and mechanical properties of wire-arc directed energy deposition 2024 aluminum alloy after adding TiC nanoparticles
title: Improvements of microstructure and mechanical properties of wire-arc directed energy deposition 2024 aluminum alloy after adding TiC nanoparticles

description: The data were obtained using conventional microstructure and mechanical properties characterization methods.
description: The data were obtained using conventional microstructure and mechanical properties characterization methods.
DOI: 10.15468/dl.3tfe36
title: Occurrence Download

description: A dataset containing 71 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "OccurrenceStatus is Present",
 "TaxonKey is Castnia eudesmia Gray, 1838"
 ]
}

 The dataset includes 71 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001128-250426092105405/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.57760/sciencedb.partic.00061
title: Nanozymes for biomedical applications in orthopaedics
title: Nanozymes for biomedical applications in orthopaedics

description: As the next generation of artificial enzymes, nanozymes have attracted increasing attention in biomedical applications due to their multienzyme-like characteristics, multifunctionalities, low cost, and high stability. By taking advantage of their diverse activities, a growing number of nanozyme-mediated therapeutic strategies have been developed for various diseases. Herein, we provide a brief review of the representative studies of nanozymes, especially in orthopaedic diseases over the past decade, which include arthritis, osteoporosis, bone regeneration, bacteria-associated infections, and osteosarcoma. Moreover, the future potential applications and some major challenges are also discussed. This review would not only provide some instructive views of nanozymes but also promote the development of enzyme-mimetic strategies in orthopaedics.
description: As the next generation of artificial enzymes, nanozymes have attracted increasing attention in biomedical applications due to their multienzyme-like characteristics, multifunctionalities, low cost, and high stability. By taking advantage of their diverse activities, a growing number of nanozyme-mediated therapeutic strategies have been developed for various diseases. Herein, we provide a brief review of the representative studies of nanozymes, especially in orthopaedic diseases over the past decade, which include arthritis, osteoporosis, bone regeneration, bacteria-associated infections, and osteosarcoma. Moreover, the future potential applications and some major challenges are also discussed. This review would not only provide some instructive views of nanozymes but also promote the development of enzyme-mimetic strategies in orthopaedics.