cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0q52p15
title: CIL:41716, Cricetulus griseus, ovary. In Cell Image Library

description: Cells were metaphase arrested with thymidine followed by colcemid, then exposed to a low ionic strength swelling medium (0.07M phosphate buffer). Chromosomes were isolated by the non-aqueous hexylene glycol technique of Wray and Stubblefield, squashed in 1:1 acetic acid:ethanol,stained, and transferred to an EM grid.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: High Voltage (1MeV) EM image of metaphase chromosomes isolated from CHO cells using a non-aqueous techniqe, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 45 degrees. The accompanying image has a tilt of 55 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.
description: Preparation: chemically fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0k936gq
title: CIL:35167, Rattus rattus, podocyte (sensu Diptera), epithelial cell. In Cell Image Library

description: Formaldehyde fixed rat kidney was cut by Vibratome and incubated with fluorescent labeled phalloidin, a toxin which binds to f-actin filaments. Imaging was performed using a BioRad MRC 600 laser scanning confocal microscope with a Kr/Ar laser. The microscope was a Nikon Diaphot with a 60X or 40X fixed tube length objective. Images of adjacent fields were manually stitched using Adobe Photoshop 2.5 on a Quadra Macintosh computer and may have manual contrast curves applied. Imaging performed at the Image Analysis Facility at the Albert Einstein College of Medicine.
description: Dominant features of a nephron in the cortex of rat kidney showing f-actin localization in the glomerulus, a blood vessel, and proximal tubules. The glomerulus is the large ball at the left. The blood vessel that delivers blood to the glomerulus (Afferent arteriole) is the bright narrow tube shown both in elongated view and in cross section (upper right) that is lined with juxtaglomerular cells. The proximal convoluted tubules are cut to reveal the actin rich micro villi lining the interior surface.
 
 This thin optical section was acquired with a laser scanning confocal microscope and is a montage of a few adjacent fields imaged at high resolution.
description: Preparation: chemically fixed tissue; vibratome-sectioned tissue
Relation to intact cell: chemically fixed tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy; fluorescence microscopy
Parameter imaged: distribution of a specific protein
Source of contrast: fluorescence emission
Visualization methods: visualization of contiguous regions; fluorescent labels
Processing history: montage
Data qualification: Processed;spatialmeasurements
DOI: 10.6075/j0ft8kcr
title: CIL:40689, Mus musculus, fibroblast. In Cell Image Library

description: High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 fibroblast, showing the fiber-like structure. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 45 degrees, providing a pair that affords an oblique stereo view of the chromosome.
description: Cells in metaphase were exposed to 75 mM KCl, spread on water, picked up on grids, stained with uranyl acetate and critical point dried.
See also:
H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96
H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: Preparation: chemically fixed tissue; critical_point dried specimen
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0sq8zpd
title: CIL:40685, Mus musculus, fibroblast. In Cell Image Library

description: Preparation: chemically fixed tissue; critical_point dried specimen
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Cells in metaphase were exposed to 75 mM KCl, spread on water, picked up on grids, stained with uranyl acetate and critical point dried.
See also:
H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96
H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 fibroblast, showing the fiber-like structure. The image was taken with a specimen tilt of 45 degrees. Grouped with it is an image with a tilt of 55 degrees, providing a pair that affords an oblique stereo view of the chromosome.
DOI: 10.6075/j0fn14tt
title: CIL:37147. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 555,000X.
description: Transmission electron micrograph of cell fractionation artificial phopsholipid membranes. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.5281/zenodo.17288179
title: Notas de leitura do processo "Proyecto de Centro Sanitario en Écija [1984]" existente no Archivo Municipal de Écija, em Écija (Sevilla).

description: Registo efetuado em 2024.08.30 no âmbito do projeto de investigação ReARQ.IB – Built Environment Knowledge for Resilient, Sustainable Communities: Understanding Everyday Modern Architecture and Urban Design in the Iberian Peninsula (1939-1985) (ERC GA949686) e publicado online em https://arquitecturaaqui.eu/pt/documentacao/processos/48927/proyecto-de-centro-sanitario-en-ecija. 

Dados exportados da plataforma Arquitectura Aqui (https://arquitecturaaqui.eu), repositório do referido projeto, em outubro de 2025.
DOI: 10.5281/zenodo.17288180
title: Notas de leitura do processo "Proyecto de Centro Sanitario en Écija [1984]" existente no Archivo Municipal de Écija, em Écija (Sevilla).

description: Registo efetuado em 2024.08.30 no âmbito do projeto de investigação ReARQ.IB – Built Environment Knowledge for Resilient, Sustainable Communities: Understanding Everyday Modern Architecture and Urban Design in the Iberian Peninsula (1939-1985) (ERC GA949686) e publicado online em https://arquitecturaaqui.eu/pt/documentacao/processos/48927/proyecto-de-centro-sanitario-en-ecija. 

Dados exportados da plataforma Arquitectura Aqui (https://arquitecturaaqui.eu), repositório do referido projeto, em outubro de 2025.
DOI: 10.6075/j0mp5230
title: CIL:37289, Streptococcus pneumoniae, bacteria. In Cell Image Library

description: A transmission electron micrograph of a diplococcus (renamed Streptococcus in 1974) pneumoniae bacteria. A striking feature of these bacteria is an intra-cytoplasmic membrane system which appears to be an extension of septa in dividing bacteria. Image is related to Figure 3 in J. Cell. Biol. 22:453-467, 1964. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: lead salt
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnificaiton X10,000.
DOI: 10.6075/j0zk5fsk
title: CIL:9983, notopthalmus viridescens, erythrocyte. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: spread nucleus
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: other negative stain
Processing history: unprocessed raw data
description: Newt (Notopthalmus viridescens erythrocytes were isolated in 0.1M KCl, spread on the surface of 5mM Na-citrate, picked up on glow discharged formvar-carbon films, fixed with glutaraldehyde and paraformaldehyde and negatively stained with 2% sodium tetraborate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 44 degrees. A grouped image of the same field at a 55 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.
DOI: 10.6075/j0sx6ccn
title: CIL:9981, notopthalmus viridescens, erythrocyte. In Cell Image Library

description: Newt (Notopthalmus viridescens erythrocytes were isolated in 0.1M KCl, spread on the surface of 5mM Na-citrate, picked up on glow discharged formvar-carbon films, fixed with glutaraldehyde and paraformaldehyde and negatively stained with 2% sodium tetraborate. Images were recorded at 20KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 43 degrees. A grouped image of the same field at a 55 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: spread nucleus
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: other negative stain
Processing history: unprocessed raw data
DOI: 10.17028/rd.lboro.30500054
title: Supplementary information files for "General-purpose machine-learned potential for 16 elemental metals and their alloys"

description: Supplementary files for article "General-purpose machine-learned potential for 16 elemental metals and their alloys"<br><br>Machine-learned potentials (MLPs) have exhibited remarkable accuracy, yet the lack of general-purpose MLPs for a broad spectrum of elements and their alloys limits their applicability. Here, we present a promising approach for constructing a unified general-purpose MLP for numerous elements, demonstrated through a model (UNEP-v1) for 16 elemental metals and their alloys. To achieve a complete representation of the chemical space, we show, via principal component analysis and diverse test datasets, that employing one-component and two-component systems suffices. Our unified UNEP-v1 model exhibits superior performance across various physical properties compared to a widely used embedded-atom method potential, while maintaining remarkable efficiency. We demonstrate our approach’s effectiveness through reproducing experimentally observed chemical order and stable phases, and large-scale simulations of plasticity and primary radiation damage in MoTaVW alloys.<br><br>©The Author(s), CC BY-NC-ND 4.0
DOI: 10.17028/rd.lboro.30500054.v1
title: Supplementary information files for "General-purpose machine-learned potential for 16 elemental metals and their alloys"

description: Supplementary files for article "General-purpose machine-learned potential for 16 elemental metals and their alloys"<br><br>Machine-learned potentials (MLPs) have exhibited remarkable accuracy, yet the lack of general-purpose MLPs for a broad spectrum of elements and their alloys limits their applicability. Here, we present a promising approach for constructing a unified general-purpose MLP for numerous elements, demonstrated through a model (UNEP-v1) for 16 elemental metals and their alloys. To achieve a complete representation of the chemical space, we show, via principal component analysis and diverse test datasets, that employing one-component and two-component systems suffices. Our unified UNEP-v1 model exhibits superior performance across various physical properties compared to a widely used embedded-atom method potential, while maintaining remarkable efficiency. We demonstrate our approach’s effectiveness through reproducing experimentally observed chemical order and stable phases, and large-scale simulations of plasticity and primary radiation damage in MoTaVW alloys.<br><br>©The Author(s), CC BY-NC-ND 4.0
DOI: 10.6075/j0ww7h0b
title: CIL:41452, Cricetulus griseus, epithelial cell. In Cell Image Library

description: High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section through a Chinese hamster ovary cell at metaphase showing a chromatid pair, with kinetochores at the centromere. The image was taken with a specimen tilt of 72 degrees and is one of a group of 10 tilts from 77 degrees to 32 degrees at 5 degree intervals. Pairs of images provide oblique stereo views of the chromosome.
description: CHO cells at metaphase were fixed with paraformaldehyde followed by OsO4, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranly acetate for HVEM observation.
See also:
H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96
H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
description: Preparation: formaldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j09w0d3h
title: CIL:37231, Cavia porcellus, epithelial cell of pancreas, acinar cell. In Cell Image Library

description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x43,000.
description: Early transmission electron micrograph of thin section of acinar cell on guinea pig pancreas. Rough endoplasmic reticulum, Golgi vesicles and portion of a zymogen granule are seen. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0tb15kd
title: CIL:37133, Rattus, capillary endothelial cell, reticulocyte. In Cell Image Library

description: Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x18,000.
description: Transmission electron micrograph of continuous endothelium from a rat diaphram capillary. Image aIso shows a reticulocyte in the capillary lumen. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0416wfw
title: CIL:41723, Cricetulus griseus, ovary. In Cell Image Library

description: High Voltage EM image of a CHO metaphase chromosome from a 'thick' section of metaphase-arrested cells treated with D20 to stabilize microtubules. This image was recorded with a specimen tilt of 45 degrees. The accompanying grouped image had a tilt of 55 degrees to create a stereo pair providing an oblique 3D view of the chromosome.
description: Preparation: chemically fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation at 1 MeV. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
DOI: 10.6075/j0c24v29
title: CIL:37228, neuron. In Cell Image Library

description: Transmission electron micrograph of a nerve cell synapsing on a dendrite. The synapse contains many synaptic vesicles and a cluster of mitochondria.
 Note that this very early (1953) micrograph is primarily of historical interest since it pre-dates glutaraldehye fixation which was introduced in the early 60's and is considered essential for adequate nerve tissue preservation. Examples of nerve tissue micrographs prepared in 1965 are CIL:37218, CIL:37219 and CIL:37220. 
 Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x35,000.
DOI: 10.6075/j028068w
title: CIL:37195, Neurospora. In Cell Image Library

description: Preparation: osmium tetroxide fixed tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: negative staining
description: References:
Palade, G.E. 1952. J. Exp. Med. 95:285-298.
Palade, G.E. 1952. Anat. Rec. 114:427-451.
Sabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58. 
Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X64,000.
description: Transmission electron micrograph of a negatively stained mitochondria from the fungi Neurospora. The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
DOI: 10.6075/j0nc5zvt
title: CIL:37135, Rattus, endothelial cell. In Cell Image Library

description: Transmission electron micrograph of endothelium caveolea. The specimen is a rat ventricular myocardium capillary. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi.
description: Relation to intact cell: freeze_fracture/freeze_etch
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
DOI: 10.6075/j0ng4p8j
title: CIL:37223, neuron. In Cell Image Library

description: Transmission electron micrograph showing the aggregation of rough endplasmic reticulum known as a Nissl bodiy within nerve cells of the superior cervical ganglion.
 Note that this very early (1953) micrograph is primarily of historical interest since it pre-dates glutaraldehye fixation which was introduced in the early 60's and is considered essential for adequate nerve tissue preservation. Examples of nerve tissue micrographs prepared in 1965 are CIL:37218, CIL:37219 and CIL:37220. 
 Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Additional reference: Palay, S and Palade, GE
J Biophys Biochem Cytol. 1955 Jan;1(1):69-88.
Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 37,500x.
description: Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data
DOI: 10.6075/j0xw4hgs
title: CIL:37156, Cavia porcellus. In Cell Image Library

description: Transmission electron micrograph of a cell fractionation of golgi bottom fraction. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.
description: Preparation: cell fractionation
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
description: The authors took advantage of the fact that rats gavage-fed a large bolus of ethanol were observed to have their Golgi cisternae loaded with LDL particles. Since LDL partlcles are lighter than most other intracellular organelles, following homognenization of ethanol-fed rats, the Golgi fractions "floated" to the top of denser sucrose gradients during centrifugation. This allowed investigators to analyze the membrane properties of "pure" Golgi fractions and set the stage for development of fusion assays that led to an understanding of factors regulating the specificity of membrane fusion - the SNARE hypothesis. An additional technical reference is Ehrenreich et al 1973 JCB 59:45-72.
Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x13,750.