cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/n5n6z-ya145
title: Shewanella OMV delMTR delDMSOR

description: Shewanella outer membrane vesicles, delMTR, del DMSO Reductase, high resolution
description: <strong>Tilt Series Date:</strong> 2018-09-14</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 82000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Lori Zacharoff in the El Naggar lab at USC; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Sample Preparation:</strong> protochip c-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, -1.5 to -5 micron defocus</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-14-5/rawdata/</p>05_dose-filt.st,  Tilt Series (Pixel Size 0.1778 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-14-5/rawdata/05_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        05_dose-filt.rec,  Reconstruction (Pixel Size 0.7112 nm), 96.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-14-5/3dimage_103122/05_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_05.mrc.mdoc,  24.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-14-5/rawdata/TS_05.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-14-5/file_20370/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 8.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-14-5/file_20371/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/69x11-59m15
title: H. Neapolitanus Carboxysomes

description: Carboxysomes purified from H. Neapolitanus, WT, high resolution
description: <strong>Tilt Series Date:</strong> 2019-08-21</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Halothiobacillus neapolitanus</p>
<strong>Strain:</strong> c2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, novaCTF, custom bash and Matlab scripts</p>
<strong>Collaborators and Roles:</strong> purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by the Savage lab</p>
<strong>Sample Preparation:</strong> protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-50/rawdata/</p>50_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 4.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-50/rawdata/50_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        50_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 405.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-50/3dimage_113111/50_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_50.mrc.mdoc,  30.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-50/rawdata/TS_50.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-50/file_26707/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 14.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-50/file_26708/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gk9zz-r8f64
title: Mega gas vesicles

description: Mega gas vesicles, purified, -1 to -5 micron defocus. Reconstruct with included scripts for ctf-corrected bin1 tomogram.
description: <strong>Tilt Series Date:</strong> 2019-09-25</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Bacillus megaterium</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-39.0°, 39.0°), step: 3°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD 4.9.8, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Rob Hurt and George Lu from the Shapiro lab at Caltech; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas with assistance from Przemek Dutka</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified Mega gas vesicles in 10 mM HEPES</p>
<strong>Sample Preparation:</strong> protochips C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-7/rawdata/</p>06_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-7/rawdata/06_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        06_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 368.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-7/3dimage_121816/06_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_06.mrc.mdoc,  28.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-7/rawdata/TS_06.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-7/file_27699/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 18.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-7/file_27700/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.wrafw7
title: Occurrence Download

description: A dataset containing 2787 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Schismus barbatus",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 2787 records from 73 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0036424-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/asj5v-0v135
title: Shewanella OMV delMTR

description: Shewanella outer membrane vesicles, delMTR (cytochromes) knockout, high resolution
description: <strong>Tilt Series Date:</strong> 2018-09-12</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 82000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Lori Zacharoff in the El Naggar lab at USC; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Sample Preparation:</strong> C-Flat 2/2-300 mesh grid, 10nm BSA-gold, 100% humidity, -1.5 to -5 micron defocus</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-23/rawdata/</p>42_dose-filt.st,  Tilt Series (Pixel Size 0.1778 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-23/rawdata/42_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        42_dose-filt.rec,  Reconstruction (Pixel Size 0.7112 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-23/3dimage_103053/42_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_42.mrc.mdoc,  24.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-23/rawdata/TS_42.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 4.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-23/file_20346/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 7.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-23/file_20347/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t47h8-1mk44
title: Coxiella burnetii_wt_D15

description: Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-15  culture.
description: <strong>Tilt Series Date:</strong> 2016-11-04</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab</p>
  dga2016-11-04-96_slicer17263.jpg: T4SS
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-96/rawdata/</p>15D_Pos4_atlas20067.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-96/rawdata/15D_Pos4_atlas20067.mrc"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20067_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-96/3dimage_108201/15D_Pos4_atlas20067_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20067.ali,  577.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-96/file_24733/15D_Pos4_atlas20067.ali"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20067.tlt,  648.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-96/file_24734/15D_Pos4_atlas20067.tlt"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20067_fid.tlt,  648.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-96/file_24735/15D_Pos4_atlas20067_fid.tlt"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16934305
title: Recursive Cascade BAO Inversion: Derived Ratios, Metrics, and Controller Outputs (v1.0)
title: RC-BAO Derived Dataset (v1.0)

description: This deposit contains the **derived data products** accompanying the manuscript:- BAO-window ratio series and metrics **before/after** Δ-match inversion- Per-scale controller outputs (λ_s)- High-z Δ_s targets (median, q25)- Trend/figure assets used in the paper
DOI: 10.15468/dl.cfq5x3
title: Occurrence Download

description: A dataset containing 4984 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Echinochloa crus-pavonis"
 ]
}

 The dataset includes 4984 records from 301 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0036422-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/g1ybj-wfn98
title: Coxiella burnetii_wt_D15

description: Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-15  culture.
description: <strong>Tilt Series Date:</strong> 2016-11-04</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-82/rawdata/</p>15D_Pos4_atlas20053.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-82/rawdata/15D_Pos4_atlas20053.mrc"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20053_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-82/3dimage_108193/15D_Pos4_atlas20053_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20053.ali,  491.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-82/file_24707/15D_Pos4_atlas20053.ali"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20053.tlt,  552.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-82/file_24708/15D_Pos4_atlas20053.tlt"
        > <i class="download icon"></i> Download </a></p>

        15D_Pos4_atlas20053_fid.tlt,  552.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-11-04-82/file_24709/15D_Pos4_atlas20053_fid.tlt"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16934306
title: Recursive Cascade BAO Inversion: Derived Ratios, Metrics, and Controller Outputs (v1.0)
title: RC-BAO Derived Dataset (v1.0)

description: This deposit contains the **derived data products** accompanying the manuscript:- BAO-window ratio series and metrics **before/after** Δ-match inversion- Per-scale controller outputs (λ_s)- High-z Δ_s targets (median, q25)- Trend/figure assets used in the paper
DOI: 10.22002/bk2ea-zam16
title: Mega gas vesicles

description: Mega gas vesicles, purified, -1 to -5 micron defocus. Reconstruct with included scripts for ctf-corrected bin1 tomogram.
description: <strong>Tilt Series Date:</strong> 2019-09-25</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Bacillus megaterium</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-39.0°, 39.0°), step: 3°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD 4.9.8, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Rob Hurt and George Lu from the Shapiro lab at Caltech; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas with assistance from Przemek Dutka</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified Mega gas vesicles in 10 mM HEPES</p>
<strong>Sample Preparation:</strong> protochips C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-8/rawdata/</p>07_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-8/rawdata/07_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        07_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-8/3dimage_121818/07_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_07.mrc.mdoc,  28.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-8/rawdata/TS_07.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-8/file_27702/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 16.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-8/file_27703/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.t8tsmu
title: Occurrence Download

description: A dataset containing 958 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Echium tuberculatum"
 ]
}

 The dataset includes 958 records from 61 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0036426-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/wj2v8-3a177
title: Mega gas vesicles

description: Mega gas vesicles, purified, -1 to -5 micron defocus. Reconstruct with included scripts for ctf-corrected bin1 tomogram.
description: <strong>Tilt Series Date:</strong> 2019-09-25</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Bacillus megaterium</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-39.0°, 39.0°), step: 3°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD 4.9.8, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Rob Hurt and George Lu from the Shapiro lab at Caltech; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas with assistance from Przemek Dutka</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified Mega gas vesicles in 10 mM HEPES</p>
<strong>Sample Preparation:</strong> protochips C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-3/rawdata/</p>02_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 2.5 GB  
        
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        02_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 368.6 MB  
        
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        TS_02.mrc.mdoc,  28.3 kB  
        
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        frames.tar.bz2,  zipped raw frames, 1.7 GB  
        
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        scripts.tar.bz2,  zipped processing scripts, 15.2 kB  
        
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