cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/nz7vp-k8634
title: Thermococcus koakarensis

description: Imaging Thermococcus koakarensis flagella
description: <strong>Tilt Series Date:</strong> 2016-08-25</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard Tk medium with starch and sulfur</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-10/rawdata/</p>Tk_250820160010.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-10/rawdata/Tk_250820160010.mrc"
        > <i class="download icon"></i> Download </a></p>

        Tk_250820160010_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-10/3dimage_78585/Tk_250820160010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4ttke-br833
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-10-17</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-17-42/rawdata/</p>Cb_d5pH4_20171017b0015.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-17-42/rawdata/Cb_d5pH4_20171017b0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_d5pH4_20171017b0015_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-17-42/3dimage_89248/Cb_d5pH4_20171017b0015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n53r1-1e176
title: perfusion_dcrp_fixed

description: This sample was fixed with 2.5% glutaraldehyde for 1 hour. The cells look sick, the outer membrane looks bad. Fixing for 1 hour turns out to be bad,need to fix for lesser time.
description: <strong>Tilt Series Date:</strong> 2015-08-18</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The sample was grown in perfusion chamber and fixed for 1hour with 2.5% glutaraldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1ul gold beads.</p>
<strong>Sample Preparation:</strong> The ice was too thick in the squares for which LM images were available, so could not correlate. Took tomograms of all bugs in a square.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-20/3dimage_60481/</p>perfusion_dcrp_atlas10020_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-20/3dimage_60481/perfusion_dcrp_atlas10020_full.rec"
        > <i class="download icon"></i> Download </a></p>

        perfusion_dcrp_atlas10020.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-20/rawdata/perfusion_dcrp_atlas10020.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.21227/z81e-0w73
title: "6G OAM-THz Channel Dataset: ITU-R IMT-2030 Compliant Physics Simulation with 33 Parameters for Deep Reinforcement Learning"

description: "The 6G OAM THz Dataset is the first comprehensive, physics-based library dedicated to Orbital Angular Momentum (OAM) beam communications at sub-Terahertz frequencies (300-600 GHz) for 6G wireless systems. This dataset includes 270,000 high-fidelity samples with perfect validation quality, as well as 33 comprehensive physics parameters covering eight critical domains: atmospheric turbulence, weather environment, fading and channel characteristics, pointing and alignment, OAM beam physics, hardware impairments, propagation physics, and performance metrics.The dataset, created using rigorous physics-based simulatio ns, covers four deployment scenarios: lab controlled (70K samples), indoor realistic (80K samples), outdoor urban (70K samples), and high mobility (50K samples), providing comprehensive coverage from controlled environments to wide-area applications. Each sample includes realistic atmospheric modeling, hardware impairments, and ambient circumstances, resulting in throughput ranges of 0.1-1000 Gbps, latency performance of 0.011-2.841 ms, and SINR coverage ranging from -30 to +50 dB.This dataset is completely consistent with DOCOMO 6G specifications and ITU-R IMT-2030 frameworks, allowing for sophisticated Deep Reinforcement Learning applications in next-generation wireless communications. Multi-agent network optimization, dynamic beam steering control, adaptive power allocation, and interference mitigation procedures are some of the primary applications. The repository includes comprehensive documentation, visualization tools, and code samples to facilitate reproducible research in 6G system design, THz propagation modeling, and OAM-based wireless communications.Keywords: 6G wireless communications, Terahertz frequency, Orbital Angular Momentum, Deep Reinforcement Learning, Physics-based simulation, DOCOMO compliance, ITU-R IMT-2030, Channel modeling, Atmospheric turbulence, Wireless dataset"
DOI: 10.22002/d0wed-bam89
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-10-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-100/rawdata/</p>Cb_vero_20171019b0097.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-100/rawdata/Cb_vero_20171019b0097.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_20171019b0097_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-100/3dimage_89629/Cb_vero_20171019b0097_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gw2by-ft570
title: Exp phase S. acidocaldarius

description: Collected sm2017-12-21-x dataset on Caltech Krios
description: <strong>Tilt Series Date:</strong> 2017-12-22</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD.</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-1/rawdata/</p>TS1_minicell.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-1/rawdata/TS1_minicell.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS1_minicell_4bin_SIRT90rot_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-22-1/3dimage_91677/TS1_minicell_4bin_SIRT90rot_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dq2rn-sw859
title: V. cholerae JAS290 delta-tcpS

description: Classical strain with ctxA deletionStrain name JAS290 (tcpS gene knockout)Cell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2016-08-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Gabriela Kovacikova</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-32/rawdata/</p>20160807_YWC217_10032.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-32/rawdata/20160807_YWC217_10032.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160807_YWC217_10032_part121_25.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-32/3dimage_76515/20160807_YWC217_10032_part121_25.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gc817-6kv73
title: Xanthomonas vs minicells

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Ec-mini cells
description: <strong>Tilt Series Date:</strong> 2017-04-27</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-53.0°, 53.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the strains, data collected by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Ec-mini cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-50/3dimage_84063/</p>Xac-mini-20170427x30003_part71_31.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-50/3dimage_84063/Xac-mini-20170427x30003_part71_31.rec"
        > <i class="download icon"></i> Download </a></p>

        Xac-mini-20170427x30003.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-27-50/rawdata/Xac-mini-20170427x30003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9j8fs-2xv44
title: V. cholerae RT4236 (refreshed)

description: Grid box AK15.Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-07-13</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 N1 pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Refreshed in fresh media right before freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: C-flat Cu 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-21/rawdata/</p>20150713_AK15_10021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-21/rawdata/20150713_AK15_10021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150713_AK15_10021_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-21/3dimage_59147/20150713_AK15_10021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f9sx6-rka23
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-10-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-27/rawdata/</p>Cb_vero_201710190025.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-27/rawdata/Cb_vero_201710190025.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_201710190025_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-27/3dimage_89503/Cb_vero_201710190025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h5cyv-qdy67
title: Lp02 ΔlspDE

description: Raw data files of Lp02 ΔlspDE
description: <strong>Tilt Series Date:</strong> 2017-03-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Nicholas Cianciotto at Northwestern University Medical School Data collected and processed by Debnath Ghosal</p>
<strong>Sample Preparation:</strong> 4 grids were frozen for this strain at OD= 3 Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-02-47/rawdata/</p>Lg_DeltaDE_201703020008.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-02-47/rawdata/Lg_DeltaDE_201703020008.mrc"
        > <i class="download icon"></i> Download </a></p>

        Lg_DeltaDE_201703020008_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-02-47/3dimage_82815/Lg_DeltaDE_201703020008_full.rec"
        > <i class="download icon"></i> Download </a></p>