cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10073495
title: The elevated gradient of aversion: a new apparatus to study the rat behavior dimensions of anxiety, fear, and impulsivity

description: Few behavioral tests allow measuring several characteristics and most require training, complex analyses, and/or are time-consuming. We present an apparatus based on rat exploratory behavior. Composed of three different environments, it allows the assessment of more than one behavioral characteristic in a short 3-min session. Factorial analyses have defined three behavioral dimensions, which we named Exploration, Impulsivity, and Self-protection. Behaviors composing the Exploration factor were increased by chlordiazepoxide and apomorphine and decreased by pentylenetetrazole. Behaviors composing the Impulsivity factor were increased by chlordiazepoxide, apomorphine, and both acute and chronic imipramine treatments. Behaviors composing the Self-protection factor were decreased by apomorphine. We submitted Wistar rats to the open-field test, the elevated-plus maze, and to the apparatus we are proposing. Measures related to exploratory behavior in all three tests were correlated. Measures composing the factors Impulsivity and Self-protection did not correlate with any measures from the two standard tests. Also, compared with existing impulsivity tests, the one we proposed did not require previous learning, training, or sophisticated analysis. Exploration measures from our test are as easy to obtain as the ones from other standard tests. Thus, we have proposed an apparatus that measured three different behavioral characteristics, was simple and fast, did not require subjects to be submitted to previous learning or training, was sensitive to drug treatments, and did not require sophisticated data analyses.
DOI: 10.6084/m9.figshare.10073483.v1
title: Improved Functionalization of Multiwalled Carbon Nanotubes in Ultra-Low Acid Volume: Effect of Solid/Liquid Interface

description: A comprehensive study of a less harmful functionalization of multiwalled carbon nanotubes (MWCNT) was performed. The influence of ultrasonic cavitation and solid/liquid interface was indirectly evaluated by statistical characterization of MWCNT aspect ratio and degree of functionalization. MWCNT aspect ratio was determined through hundreds of electron microscopy images. Degree of functionalization of MWCNT was characterized by elemental analysis and thermogravimetry. Distribution of different oxygen-containing functional groups was assessed by potentiometric titration. MWCNT aqueous dispersions were evaluated by electrophoretic mobility and dynamic light scattering. An acid volume over 90% lower than usually reported in literature guaranteed MWCNTs with significantly larger aspect ratio, higher proportion of carboxylic groups and a more negative zeta potential in aqueous suspension. These results can be related to a more localized ultrasonic cavitation in MWCNTs/acid interface for reduced liquid volumes. This distinguished influence of ultrasonic cavitation may be a valuable contribution to more eco-friendly processes with nanomaterials.
DOI: 10.6084/m9.figshare.10073495.v1
title: The elevated gradient of aversion: a new apparatus to study the rat behavior dimensions of anxiety, fear, and impulsivity

description: Few behavioral tests allow measuring several characteristics and most require training, complex analyses, and/or are time-consuming. We present an apparatus based on rat exploratory behavior. Composed of three different environments, it allows the assessment of more than one behavioral characteristic in a short 3-min session. Factorial analyses have defined three behavioral dimensions, which we named Exploration, Impulsivity, and Self-protection. Behaviors composing the Exploration factor were increased by chlordiazepoxide and apomorphine and decreased by pentylenetetrazole. Behaviors composing the Impulsivity factor were increased by chlordiazepoxide, apomorphine, and both acute and chronic imipramine treatments. Behaviors composing the Self-protection factor were decreased by apomorphine. We submitted Wistar rats to the open-field test, the elevated-plus maze, and to the apparatus we are proposing. Measures related to exploratory behavior in all three tests were correlated. Measures composing the factors Impulsivity and Self-protection did not correlate with any measures from the two standard tests. Also, compared with existing impulsivity tests, the one we proposed did not require previous learning, training, or sophisticated analysis. Exploration measures from our test are as easy to obtain as the ones from other standard tests. Thus, we have proposed an apparatus that measured three different behavioral characteristics, was simple and fast, did not require subjects to be submitted to previous learning or training, was sensitive to drug treatments, and did not require sophisticated data analyses.
DOI: 10.6084/m9.figshare.10073474
title: Torsional comparative biomechanical test of modified tibial-plateau-leveling osteotomy plate and locking plate in canine synthetic tíbias

description: ABSTRACT The objective of this study was to develop a locked tibial-plateau-leveling osteotomy (TPLO) plate and to compare this implant with the conventional bone fixation method using a locked plate to determine bone stabilization against torsion forces. Maximum force, angle at peak torque, and stiffness values were determined. Ten synthetic tibias were used, divided into 2 groups. The results in Group 1 (modified TPLO plate) and Group 2 (locked plate) were assessed using analysis of variance and the means were compared using Tukey’s test at 5% probability. There were significant differences in the angle at peak torque and stiffness. The group Modified TPLO plate had higher mean values of stiffness compared with Group locked plate. Group locked plate showed a greater angle at peak torque compared with Group modified TPLO plate. All constructs failed due to tibial fractures distal to the plate. The modified TPLO plate presented higher stiffness indexes than conventional locked plate in torsion. The locked plate presented greater elasticity than modified TPLO plate having greater angle at the peak of torque.
DOI: 10.6084/m9.figshare.10073480
title: Uterine intussusception in a crossbred female dog - case report

description: ABSTRACT The present study describes a rare condition of uterine intussusception in a 4 year old crossbred female dog diagnosed by exploratory celiotomy and anatomopathological analysis. As treatment, a ovariohysterectomy (OH) was performed. This pathology usually requires surgical management because the initial diagnosis can be challenging.
DOI: 10.6084/m9.figshare.10073468
title: Multi-Energy Calibration: A Practical Method for Determination of Macro and Micro Nutrients in Meat by ICP OES

description: The multi-energy calibration (MEC) was evaluated for the determination of Ca, Cu, Fe, K, Mg, Na, P, S, and Zn in meat samples by inductively coupled plasma optical spectrometry (ICP OES). This calibration method consists of using only two calibration standards and several atomic and ionic emission wavelengths with different sensitivities to determine the analyte concentration in the sample. Both calibration mixtures are prepared using the same amount of sample, which contributes to minimize matrix effects. The accuracy was evaluated using two certified reference materials, bovine liver (SRM 1577c) and bovine muscle (SRM 8414), with recoveries within 87-107% range. The method was applied for the determination of the analytes in meats of chicken, sheep loin, sheep carcass and bovine, and prove to be usable in samples with different characteristics.
DOI: 10.6084/m9.figshare.10073468.v1
title: Multi-Energy Calibration: A Practical Method for Determination of Macro and Micro Nutrients in Meat by ICP OES

description: The multi-energy calibration (MEC) was evaluated for the determination of Ca, Cu, Fe, K, Mg, Na, P, S, and Zn in meat samples by inductively coupled plasma optical spectrometry (ICP OES). This calibration method consists of using only two calibration standards and several atomic and ionic emission wavelengths with different sensitivities to determine the analyte concentration in the sample. Both calibration mixtures are prepared using the same amount of sample, which contributes to minimize matrix effects. The accuracy was evaluated using two certified reference materials, bovine liver (SRM 1577c) and bovine muscle (SRM 8414), with recoveries within 87-107% range. The method was applied for the determination of the analytes in meats of chicken, sheep loin, sheep carcass and bovine, and prove to be usable in samples with different characteristics.
DOI: 10.6084/m9.figshare.10073474.v1
title: Torsional comparative biomechanical test of modified tibial-plateau-leveling osteotomy plate and locking plate in canine synthetic tíbias

description: ABSTRACT The objective of this study was to develop a locked tibial-plateau-leveling osteotomy (TPLO) plate and to compare this implant with the conventional bone fixation method using a locked plate to determine bone stabilization against torsion forces. Maximum force, angle at peak torque, and stiffness values were determined. Ten synthetic tibias were used, divided into 2 groups. The results in Group 1 (modified TPLO plate) and Group 2 (locked plate) were assessed using analysis of variance and the means were compared using Tukey’s test at 5% probability. There were significant differences in the angle at peak torque and stiffness. The group Modified TPLO plate had higher mean values of stiffness compared with Group locked plate. Group locked plate showed a greater angle at peak torque compared with Group modified TPLO plate. All constructs failed due to tibial fractures distal to the plate. The modified TPLO plate presented higher stiffness indexes than conventional locked plate in torsion. The locked plate presented greater elasticity than modified TPLO plate having greater angle at the peak of torque.
DOI: 10.6084/m9.figshare.10073462
title: Nutritional intervention may improve migraine severity: a pilot study

description: ABSTRACT Although some studies have investigated the role of nutritional intervention on migraine, they had focused on triggers or on weight change and, to the best of our knowledge, none studied diet quality. Objective To investigate whether nutritional intervention focused on improving diet quality and healthy weight can promote improvement in clinical parameters of women with migraine. Methods Non-controlled and non-randomized intervention study conducted for 90 days. Women received an individualized diet meal plan and nutritional orientation according to their nutritional diagnosis. Anthropometric, clinical and nutritional data were measured once a month. Diet energy content and macronutrients were evaluated using 24-hour dietary recall. Diet quality was assessed through the Brazilian Healthy Eating Index–Revised (BHEI-R). The Migraine Disability Assessment and Headache Impact Test version 6 were used to assess the severity of migraine, and the Beck Depression Inventory evaluated depressive symptoms. Results Fifty-two women aged 44.0 ± 13.0 years were enrolled. Anthropometric characteristics, energy, macronutrients and fiber intake did not change after intervention. However, the BHEI-R scores improved after 60 and 90 days of intervention. Concurrent to this, the Beck Depression Inventory scores and Headache Impact Test scores decreased after 60 and 90 days, respectively. The change in the BHEI-R score was negatively correlated with the migraine severity as assessed by the Headache Impact Test at the end of the intervention. Conclusions We concluded that the management of diet quality may be a good strategy for improving migraine severity, regardless of the nutritional status and weight change.
DOI: 10.6084/m9.figshare.10073480.v1
title: Uterine intussusception in a crossbred female dog - case report

description: ABSTRACT The present study describes a rare condition of uterine intussusception in a 4 year old crossbred female dog diagnosed by exploratory celiotomy and anatomopathological analysis. As treatment, a ovariohysterectomy (OH) was performed. This pathology usually requires surgical management because the initial diagnosis can be challenging.
DOI: 10.6084/m9.figshare.10073447
title: Pre-experimental Stimuli Functions in Equivalence Class Formation

description: Abstract Can pre-experimental conditional relations that elicit emotional responses, such as jealousy, affect the formation of equivalence classes? Would there be differences between men and women? To address these questions, 19 participants of both sexes were exposed to training and testing of the formation of four equivalence classes, one consisting of the boyfriend’s/girlfriend’s name, an unknown woman’s/man’s name, and two unfamiliar figures. After the tests, the Romantic Jealousy Scale was applied. Participants classified as having a higher degree of jealousy did not form equivalence classes regardless of whether they were men or women. It is suggested that pre-experimental relations with affective functions interfere in the formation of equivalence classes in which the boyfriend’s/girlfriend’s name should be related to the unknown woman’s/man’s name.
DOI: 10.6084/m9.figshare.10073465
title: Investigating the Emergence of Equivalent Relationships with Mutually Exclusive Training Structures

description: ABSTRACT This study investigated equivalence classes formation from conditional discrimination training structures that correspond to mutually exclusive emerging relationships. A total of 10 college students participated in two experiments. Trained relations were B1A1, B2A2, B3A3, D1C1, D2C2, D3C3, B1C2, B2C1, B3C3, D1A1, D2A2 and D3A3. Relations AC, CA, BD and DB were tested. Experiment II differed from Experiment I in that it included a review of training relations. The results indicated class formation for seven of the 10 participants, without significant differences between the experiments. However, different classes were established for the participants of both experiments, suggesting that the training order was not enough to determine which equivalence classes would be formed.
DOI: 10.6084/m9.figshare.10073453
title: Brain adaptations during a long-time task: An Event-Related Potential (ERP) study.

description: Abstract The aim of this study is to investigate the effect of prolonged cognitive demand in Event Related Potential (ERP) modulation in inhibitory control paradigm. Data were collected in 19 right-handed volunteers with mean age of 21.21 (±1,77) years who performed the Go/NoGo paradigm for 50 minutes, with synchronized electroencephalogram recording to obtain the ERPs. The time on task effect induced significant changes in subjective variable, cognitive performance and maximum amplitudes of the N2 and P3 component. Our results suggest that when our brain is subjected to extensive cognitive demands, there are adaptations to maintain the behavioral performance through strategy reallocation of energy resources.
DOI: 10.6084/m9.figshare.10073462.v1
title: Nutritional intervention may improve migraine severity: a pilot study

description: ABSTRACT Although some studies have investigated the role of nutritional intervention on migraine, they had focused on triggers or on weight change and, to the best of our knowledge, none studied diet quality. Objective To investigate whether nutritional intervention focused on improving diet quality and healthy weight can promote improvement in clinical parameters of women with migraine. Methods Non-controlled and non-randomized intervention study conducted for 90 days. Women received an individualized diet meal plan and nutritional orientation according to their nutritional diagnosis. Anthropometric, clinical and nutritional data were measured once a month. Diet energy content and macronutrients were evaluated using 24-hour dietary recall. Diet quality was assessed through the Brazilian Healthy Eating Index–Revised (BHEI-R). The Migraine Disability Assessment and Headache Impact Test version 6 were used to assess the severity of migraine, and the Beck Depression Inventory evaluated depressive symptoms. Results Fifty-two women aged 44.0 ± 13.0 years were enrolled. Anthropometric characteristics, energy, macronutrients and fiber intake did not change after intervention. However, the BHEI-R scores improved after 60 and 90 days of intervention. Concurrent to this, the Beck Depression Inventory scores and Headache Impact Test scores decreased after 60 and 90 days, respectively. The change in the BHEI-R score was negatively correlated with the migraine severity as assessed by the Headache Impact Test at the end of the intervention. Conclusions We concluded that the management of diet quality may be a good strategy for improving migraine severity, regardless of the nutritional status and weight change.
DOI: 10.6084/m9.figshare.10073465.v1
title: Investigating the Emergence of Equivalent Relationships with Mutually Exclusive Training Structures

description: ABSTRACT This study investigated equivalence classes formation from conditional discrimination training structures that correspond to mutually exclusive emerging relationships. A total of 10 college students participated in two experiments. Trained relations were B1A1, B2A2, B3A3, D1C1, D2C2, D3C3, B1C2, B2C1, B3C3, D1A1, D2A2 and D3A3. Relations AC, CA, BD and DB were tested. Experiment II differed from Experiment I in that it included a review of training relations. The results indicated class formation for seven of the 10 participants, without significant differences between the experiments. However, different classes were established for the participants of both experiments, suggesting that the training order was not enough to determine which equivalence classes would be formed.
DOI: 10.6084/m9.figshare.10073447.v1
title: Pre-experimental Stimuli Functions in Equivalence Class Formation

description: Abstract Can pre-experimental conditional relations that elicit emotional responses, such as jealousy, affect the formation of equivalence classes? Would there be differences between men and women? To address these questions, 19 participants of both sexes were exposed to training and testing of the formation of four equivalence classes, one consisting of the boyfriend’s/girlfriend’s name, an unknown woman’s/man’s name, and two unfamiliar figures. After the tests, the Romantic Jealousy Scale was applied. Participants classified as having a higher degree of jealousy did not form equivalence classes regardless of whether they were men or women. It is suggested that pre-experimental relations with affective functions interfere in the formation of equivalence classes in which the boyfriend’s/girlfriend’s name should be related to the unknown woman’s/man’s name.
DOI: 10.6084/m9.figshare.10073441
title: Genotoxic and Genoprotective Potential of Black Mulberry (Morus nigra) Fruit

description: Abstract: Black Mulberry (Morus nigra L.) belongs to Moraceae family. The present study evaluated the possible genotoxic and/or protective activities of black mulberry fruit juice (BMFJ), in vitro, using mitomycin C (MMC) as a positive control, by chromosomal aberrations and micronucleus assays. Human lymphocytes were treated with BMFJ concentrations alone (1/1, 1/2, 1/4, 1/8 dilutions), pretreatment (49h) (0.20 μg/ml MMC+ 1/1 BMFJ, 0.20 μg/ml MMC+1/2 diluted BMFJ, 0.20 μg/ml MMC+1/4 diluted BMFJ, 0.20 μg/ml MMC+1/8 diluted BMFJ) and simultaneous-treatment (48h) (0.20 μg/ml MMC+ 1/1 BMFJ, 0.20 μg/ml MMC+1/2 diluted BMFJ, 0.20 μg/ml MMC+1/4 diluted BMFJ, 0.20 μg/ml MMC+1/8 diluted BMFJ). The in vitro results demonstrated that BMFJ showed no genotoxicity, but it significantly decreased chromosomal aberration and micronucleus frequency induced by MMC. Our results showed that all concentrations of BMFJ revealed no genotoxicity but protective activity against genomic changes induced by anti-tumor agent MMC in human lymphocytes. Protective effects of BMFJ on MMC induced chromosomal damages most probably due to its free radical scavenging activity.
DOI: 10.6084/m9.figshare.10073453.v1
title: Brain adaptations during a long-time task: An Event-Related Potential (ERP) study.

description: Abstract The aim of this study is to investigate the effect of prolonged cognitive demand in Event Related Potential (ERP) modulation in inhibitory control paradigm. Data were collected in 19 right-handed volunteers with mean age of 21.21 (±1,77) years who performed the Go/NoGo paradigm for 50 minutes, with synchronized electroencephalogram recording to obtain the ERPs. The time on task effect induced significant changes in subjective variable, cognitive performance and maximum amplitudes of the N2 and P3 component. Our results suggest that when our brain is subjected to extensive cognitive demands, there are adaptations to maintain the behavioral performance through strategy reallocation of energy resources.
DOI: 10.6084/m9.figshare.10073438
title: Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

description: ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated shortly after follicular aspiration, 79.00% were in GV, GVBD, MI, while 13.40%, were in MII and 7.60%, D/NI. Significant difference was observed in different concentrations (T100, T200 and T150µM) in oocytes that have reached the MII phase compared to control treatments (P= 0.003). Differences were observed in cleavage rate (P&lt; 0.05) between T150 and T200 when compared to the C/24 Group. A high difference was observed on blastocyst rate (P&lt; 0.001) among treatments compared to the control group.
DOI: 10.6084/m9.figshare.10073438.v1
title: Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

description: ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated shortly after follicular aspiration, 79.00% were in GV, GVBD, MI, while 13.40%, were in MII and 7.60%, D/NI. Significant difference was observed in different concentrations (T100, T200 and T150µM) in oocytes that have reached the MII phase compared to control treatments (P= 0.003). Differences were observed in cleavage rate (P&lt; 0.05) between T150 and T200 when compared to the C/24 Group. A high difference was observed on blastocyst rate (P&lt; 0.001) among treatments compared to the control group.