DOI: 10.5061/dryad.0p2ngf28f
title: Data from: Humidity modifies age-dependent heat wave effects in an insect host-parasitoid interaction

description: Climate change is projected to increase the likelihood of extreme heat
 events, but it may also increase humidity levels, leading to the potential
 for coupled thermal and hydric stress. While increasing frequency and
 intensity of extreme heat events have been well-documented for their
 negative effects on species and their interactions, how humidity modulates
 the impacts of heat stress is currently unknown.  We investigated
 how humidity interacted with heat stresses of different timings and
 durations to affect the life histories in an insect host-parasitoid
 interaction, comprising the Indian meal moth, Plodia
 interpunctella, and its endoparasitoid wasp, Venturia canescens. Hosts
 parasitised as 4th instar larvae and unparasitized hosts were maintained
 in a high humid (60.8% RH) or low-humid (32.5% RH) environment at a
 constant temperature of 28℃. They were then exposed to 38℃ thermal
 stresses with a duration of 6 or 72 hours in either the 4th or 5th instar.
 Humidity and heat stresses did not affect the adult emergence of
 unparasitized hosts, but longer heat stress and lower humidity increased
 the probability that host adults emerged from parasitized hosts,
 indicating the negative effect of these conditions on the survival of
 parasitoids. Furthermore, juvenile development time and body size of hosts
 and parasitoids responded differently to timing and duration of heat
 stresses, and high humidity decreased larval development time of
 unparasitized hosts and increased the size of both hosts and parasitoids.
 In addition, humidity negatively interacted with larval stage and duration
 of heat stress to affect the size of unparasitized hosts, but this effect
 was not found in parasitoids. Our results show that humidity modulates the
 life history of hosts and parasitoids, highlighting the importance of
 humidity in maintaining host-parasitoid interactions. Humidity should be
 considered when predicting the impact of temperature extremes on species’
 population dynamics and their interactions.
description: This data were collected from a lab-based study investigating the
 impact of humidity on the responses of host-parasitoid to heat
 stresses.
description: # Data from: Humidity modifies age-dependent heat wave effects in an
 insect host-parasitoid interaction
 [https://doi.org/10.5061/dryad.0p2ngf28f](https://doi.org/10.5061/dryad.0p2ngf28f) This data set corresponding to the paper: Li D, Brough B, Rees JW, Coste CFD, Yuan C, Fowler MS, Sait SM. Humidity modifies species-specific and age-dependent heat stress effects in an insect host-parasitoid interaction. Ecol Evol. 2024 Jul 21;14(7):e70047. doi: 10.1002/ece3.70047. PMID: 39035041; PMCID: PMC11260500. ## Description of the data and file structure This contains two data files:  **Host.csv**---life history measures of the host. **Parasitoid.csv** - life history measures of the parasitoid. \~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Within each data set, there are several columns indicate different treatments and measures for each individual of host and parasitoid:  `Plate_id`: the id of 25-well plate used for the experiment.  `Id`: unique id of each individual larvae.  `Parasitism`: Whether hosts are parasitized by parasitoids. H = host alone, HP = hosts were parasitized by parasitoids. `Duration`: Duration of heat stress i.e., 0, 6, 72 hours. Unite = hour `Stage`: Host stages when heat stress exposed i.e., L4 = 4th instar, L5 = 5th instar.  `Eggtransfer_date`: The date when eggs were transferred into plates i.e., the first day of observation. Unit = date. `Survivived2L4`: If individual host larval has survived to 4th instar. 1 = yes, 0 = no.  `Pupate`: If individual host larval has successfully pupated. 1 = yes, 0 = no. NA when hosts did not survive earlier.  `Hostpupate_date`: When hosts were first observed as pupated. NA when hosts did not survive earlier. Unit: date `Hostemergence/Wasp_emergence`: If individual host/parasitoid adult has successfully emerged. 1 = yes, 0 = no. NA when host/parasitoid did not survive to this stage. `Hostemerge_date`: When host adults were emerged. NA when hosts did not survive earlier. Unit = date `Hostdead_date`: When host adults were recorded dead. NA when hosts did not survive earlier. Unit = date. `Host_sex`: The sex of host i.e., M= male, F = femal. NA when hosts did not survive earlier.  `Midfemur`: The mid-femur length of host adults. NA when not applicable. Unit = mm. `Rearfemur:` The rear-femur length of host adults. NA when not applicable. Unit = mm. `Wasp_size`: The hind tibia length of parasitoid. Unit = mm. ## Sharing/Access information Please refer to the original publication: Li D, Brough B, Rees JW, Coste CFD, Yuan C, Fowler MS, Sait SM. Humidity modifies species-specific and age-dependent heat stress effects in an insect host-parasitoid interaction. Ecol Evol. 2024 Jul 21;14(7):e70047. doi: 10.1002/ece3.70047. PMID: 39035041; PMCID: PMC11260500. Links to other publicly accessible locations of the data: * Github: [https://github.com/Dongboli/experimental-data](https://github.com/Dongboli/experimental-data) Data was derived from the following sources: * Lab study