DOI: 10.6084/m9.figshare.5936287.v2
title: Drosophila wing images from a phenotypic selection gradient experiment with lineages evolved with predators.

description: These are images of wings from populations of <i>Drosophila melanogaster </i>experimentally evolved in the presence (or absence for controls) of juvenile Chinese mantids <i>(Tenodera aridifolia sinensis</i>) for 32 generations.<br>There are two control lineages and two selection lineages (i.e. 2 replicate populations for each of the predator treatment and the control).<br>Flies were reared in a common garden for two generations prior to the experiment used for the phenotypic selection gradient analysis.<br>These experiments were done by Dr. Michael DeNieu in the lab of Ian Dworkin at Michigan State University in 2010. <br>Each zipped folder contains a subset of the images. This was done to help with uploading to Figshare.<br><br><br>Assaying Phenotypic Selection: Evolved populations<br>To examine how the fitness function changed as a result of experimental evolution, we<br>repeated phenotypic selection (as described above) during generations 31 and 32 of the<br>experiment. Given the large size of this experiment, it was performed in four blocks,<br>with two blocks for each generation. At generation 31 of experimental evolution, we set<br>up 14 arenas each of PredR1 females & males and ConR1 females & males. Five days<br>later, we set up 14, 14, 8, and 9 arenas for PredR2 females & males and ConR2 females &<br>males respectively. At generation 32, we set up 14 arenas each of PredR1 females & males<br>and ConR1 females & males . Five days later we set up 13, 13, 14, and 14 arenas for<br>PredR2 females & males and ConR2 females & males respectively. As before, we collected<br>all surviving flies and unconsumed wings and stored them in ethanol. Overlapping egg<br>cases were used for this experiment, and egg case of origin was used as a covariate in the<br>model (see below). We distributed mantids so as not to confound predation effects across<br>replicates and treatments.<br><br>Wing imaging<br>Wings were dissected and mounted on slides in 70% glycerol. When available, both<br>wings from an individual were mounted. Wings were also dissected from 25 flies that<br>were stored from the initial generation of experimental evolution, and from every 10 generations following up to generation 30 to estimate the trajectory of size and shape<br>change. Wings were imaged with a total 40X magnification on an Olympus DP30BW camera<br>mounted on a Olympus BX51 microscope using ‘DP controller’ V3.1.1 software. All<br>images were saved in greyscale as tiff files.<br><br>** Note ** in preparing these images for upload to figshare I noticed a small set were saved as .jpg.<br><br>See the pre-print<br>https://www.biorxiv.org/content/early/2014/05/19/005322.short for more details about the experimental protocol.<br><br><br>Naming convention for the images. Variables are seperated by underscore<br><br>SEL/CON - selection or control treatments. This refers to the experimental evolution lineages.<br>M/F - Sex of individuals<br>R1/R2 - Replicate lineage for experimental evolution<br>B1/B2 - Block one or block two. These are the experimental blocks used for performing the phenotypic selection analysis.<br>S/D - Whether the individual survived the predation assay for phenotypic selection or died (was preyed upon by the mantid).<br>number - unique number for each fly (I believe)<br>L/R - Left or Right wing. We don't have this for most flies that were preyed upon (i.e. had a code of D).<br><br>So<br>CON_F_R2_B2_D_4520_L.tif<br><br>would be control lineage female replicate 2 block 2 dead left wing.<br><br><br>and<br>SEL_M_R1_B1_S_2120_R.jpg<br>Would be selection lineage male replicate 1 block 1 survivor right wing.<br><br>