DOI: 10.5061/dryad.m874vg5
title: Data from: Floral organs act as environmental filters and interact with pollinators to structure the yellow monkeyflower (Mimulus guttatus) floral microbiome

description: Assembly of microbial communities is the result of neutral and selective
 processes. However, the relative importance of these processes is still
 debated. Microbial communities of flowers, in particular, have gained
 recent attention because of their potential impact to plant fitness and
 plant-pollinator interactions. However, the role of selection and
 dispersal in the assembly of these communities remains poorly understood.
 We evaluated the role of pollinator-mediated dispersal on the contribution
 of neutral and selective processes in the assembly of floral microbiomes
 of the yellow monkeyflower (Mimulus guttatus). We sampled flowers in the
 presence and absence of pollinators within five different serpentine seeps
 in CA and obtained 16s amplicon data on the epiphytic bacterial
 communities. Consistent with strong micro-environment selection within
 flowers we observed significant differences in community composition
 across floral organs and only a small effect of geographic distance.
 Pollinator exposure affected the contribution of environmental selection
 and depended on the rate and “intimacy” of interactions with flower
 visitors. This study provides evidence of the importance of dispersal and
 within-flower heterogeneity in shaping epiphytic bacterial communities of
 flowers, and highlights the complex interplay between pollinator behavior,
 environmental selection and additional abiotic factors in shaping the
 epiphytic bacterial communities of flowers.
description: # Data from: Floral organs act as environmental filters and interact with
 pollinators to structure the yellow monkeyflower (Mimulus guttatus) floral
 microbiome
 [https://doi.org/10.5061/dryad.m874vg5](https://doi.org/10.5061/dryad.m874vg5) ## Description of the data and file structure ### Files and variables #### Environmental covariates Pollinator data, co-flowering community * **PollinatorData.csv** : Pollination observations data. Collected by Maria Rebolleda-Gomez and Rebecca Hayes. * Location: The number of location within seep (see figure 1 of paper, and supplementary table for coordinates). * Seep: One of the five replicate seeps in the study (BNS, RH1, RHA, RHB, TP9; see figure 1 of paper and supplementary table for coordinates). * Visits: Number of visits in 15 min. * Functional group: Functional pollinator categories from Koski et al., 2015: XS- extra-large social bees; LS- Large social bees; SB- small solitary bee; MB- medium solitary bee; LB- large solitary bee (pollen on body); LL- large solitary bee (pollen on legs); BF- bee fly; SF- small syrphid fly; LF- large syrphid fly; FL- other flies; LE- moths and butterflies; BE- beetles; VE- wasps; OT- other. * Mimulus: If pollinator visited Mimulus or not “No”. If it did visit a M. guttatus flower it was recorded as either “In” if it went inside the flower “In” or stayed “Out”. * Hour: Time of the day at which the observation was finished. * Block: There were two blocks done almost a week apart from each other, sequencing samples were collected together with the second block. * Time: 1-morning; 2-afternoon * Day: Date in Julian * **FlowerAbundance_170510.csv** : Observations of co-flowering community on May X-Y. Initial days of the experiment around the time I set the pollinator exclusion cages. * Columns indicate the different locations: The first three letters are the seep identifier see figure 1 of paper , and supplementary table for coordenates). * Rows indicate the flowering species. Species are in codes of four letters (see species code file). * **FlowerAbundance_170516_Block2.csv** : Observations of co-flowering community on May X-Y. Around the time I sampled flowers in the field. **Rows and columns as above**. #### Sample ID key Sample that corresponds to the file ID in the demultiplex samples from ARGONNE. #### MIGU\_metadata.txt Data about the samples: * SampleID: same as in sequences and OTU tables * BarcodeSequence: from sequencing * LinkerPrimerSequence: from sequencing * BarcodePlate: Number of sequencing plate * Well: Well in plate for sequencing * Seep: Seep codes (BNS, RH1, RHA, RHB, TP9; see figure 1 of paper and supplementary table for coordenates). * Loc: Location within seep (1-3, or 1-4; see figure 1 of paper and supplementary table for coordenates).) * StigmaOpen: Number of flowers with open stigmas in sample * Pollinator: Pollinator treatment (NP-No pollinator (pollinator exclusion); WP- with pollinators (control)) * Organ: Where did the sample come from? Style, Anthers (Stamens), Petals, Community, Soil or Leaves. * Type: Endo (endophytes, sequence after wahing and then lysing tissue), Wash (sequenced after washing the sample) * Description: Seep_Location_Treatment_Organ_Type #### Locations.csv Coordinates of our seeps and locations.