DOI: 10.5061/dryad.51c59zwkt
title: Linked song and preference loci suggest substantial contribution of genetic coupling in rapid speciation of the <em>Laupala</em> crickets

description: Species-specific sexual signals and preferences are central to the
 evolutionary origins and maintenance of reproductive barriers. How such
 communication systems evolve is key to understanding speciation. One
 central question considers how signals and preferences coevolve within
 lineages while each diverges among lineages. Here, we study the rapidly
 speciating Laupala cricket, a system characterized by coordinated sexual
 signaling. By fine-mapping variation for both the pulse rate of the male
 calling song and female pulse rate preference, we examine the genetic
 architecture underlying signal-preference divergence. We document a pair
 of colocalizing pulse rate and preference QTL on linkage group 4, with
 peak locations less than 2 cM apart, offering strong evidence for genetic
 coupling. Intriguingly, this is the third pair of colocalizing male and
 female loci mapped in the Laupala genome. The cumulative effect size of
 the three pairs of colocalizing loci accounts for roughly half of the
 interspecific difference, suggesting that genetic coupling has contributed
 substantially to the evolution of this behavioral barrier in Laupala.
 Annotation of the QTL region identified numerous functionally relevant
 candidate genes, including acetylcholine esterase (AChE) and ryanodine
 receptor (RyR).
description: # Linked song and preference loci suggest substantial contribution of
 genetic coupling in rapid speciation of the *Laupala* crickets Dataset
 DOI: [10.5061/dryad.51c59zwkt](10.5061/dryad.51c59zwkt) ## Description of
 the data and file structure Eight files contain original data inputted to
 r/QTL for QTL mapping using the integrated map (3e_int*) and individual
 family map (3e.X*): ·      **3e_int_map3_miss20_f_input.csv** ·     
 **3e_int_map3_miss20_m_input.csv** ·     
 **3e.4_f_miss20_lg4map3_othermap2_qtl_input.csv** ·     
 **3e.4_m_miss20_lg4map3_othermap2_qtl_input.csv** ·     
 **3e.6_f_miss20_lg4map3_othermap2_qtl_input.csv** ·     
 **3e.6_m_miss20_lg4map3_othermap2_qtl_input.csv** ·     
 **3e.8_f_miss20_lg4map3_othermap2_qtl_input.csv** ·     
 **3e.8_m_miss20_lg4map3_othermap2_qtl_input.csv** All files are in the
 required input format for R package r/QTL 
 
 ### Files and variables #### File: 3e_int_map3_miss20_f_input.csv
 **Description:** input data for QTL mapping using combined family data on
 the integrated map for females #### File:
 3e.4_f_miss20_lg4map3_othermap2_qtl_input.csv **Description:** input data
 for QTL mapping for 3E.4 females #### File: 3e_int_map3_miss20_m_input.csv
 **Description:** input data for QTL mapping using combined family data on
 the integrated map for males #### File:
 3e.8_f_miss20_lg4map3_othermap2_qtl_input.csv **Description:** input data
 for QTL mapping for 3E.8 females #### File:
 3e.8_m_miss20_lg4map3_othermap2_qtl_input.csv **Description:** input data
 for QTL mapping for 3E.8 males #### File:
 3e.6_f_miss20_lg4map3_othermap2_qtl_input.csv **Description:** input data
 for QTL mapping for 3E.6 females #### File:
 3e.6_m_miss20_lg4map3_othermap2_qtl_input.csv **Description:** input data
 for QTL mapping for 3E.6 males #### File:
 3e.4_m_miss20_lg4map3_othermap2_qtl_input.csv **Description:** input data
 for QTL mapping for 3E.4 males