DOI: 10.5061/dryad.652r5
title: Data from: Fine scale genetic structure among greater sage-grouse leks in central Nevada

description: Background: Mating systems that reduce dispersal and lead to non-random
 mating might increase the potential for genetic structure to arise at fine
 geographic scales. Greater sage-grouse (Centrocercus urophasianus) have a
 lek-based mating system and exhibit high site fidelity and skewed mating
 ratios. We quantified population structure by analyzing variation at
 27,866 single-nucleotide polymorphisms in 140 males from ten leks (within
 five lek complexes) occurring in a small geographic region in central
 Nevada. Results: Lek complexes, and to a lesser extent individual leks,
 formed statistically identifiable clusters in ordination analyses,
 providing evidence for fine-scale geographic genetic differentiation. Lek
 geography predicted genetic differentiation even at a small geographic
 scale, which could be sharpened by strong site fidelity. Relatedness was
 also higher among individuals within lek complexes (and leks), suggesting
 that reproductive skew, where few males participate in most of the
 successful matings, could also potentially contribute to genetic
 differentiation. Models incorporating a habitat resistance surface as a
 proxy for potentially reduced movement due to landscape features indicated
 that both geographic distance and habitat suitability (i.e. preferred
 habitat) predicted genetic structure, with no significant effect of
 man-made barriers to movement (i.e. power lines and roads). Finally, we
 illustrate how data sets containing fewer loci (&lt;4000) had less
 statistical precision and failed to detect the full degree of genetic
 structure. Conclusion: Our results suggest that habitat features and lek
 site geography of sage-grouse shape fine scale genetic structure, and
 highlight how larger data sets can have increased precision and accuracy
 for quantifying ecologically relevant genetic structure over small
 geographic scales.
description: Illumina library prep
 protocolThis file contains the
 protocol for constructing Illumina libraries for
 genotyping-by-sequencing.RFseq_protocol.pdfMatrix of genotype probabilitiesThis file contains a matrix of genotype probabilities and individual ids (leks and lek complexes) for the 140 sage-grouse included in the final downstream analyses. Genotype probabilities range from 0 to 2, with 0s and 2s representing homozygotes and 1s representing heterozygotes.gprob_ids_complex.txtHorse Creek bamsThis file contains the sorted .bam files for individuals from the Horse Creek lek.CO_HC_bams.tgzQuartz Road bamsThis file contains the sorted .bam files for individuals from the Quartz Road lek.CO_QR_bams.tgzKobeh Valley bamsThis file contains the sorted .bam files for individuals from the Kobeh Valley lek.KV_KB_bams.tgzLone Mountain bamThis file contains the sorted .bam files for individuals from the Lone Mountain lek.KV_LM_bams.tgzModarelli Mine bamsThis file contains the sorted .bam files for individuals from the Modarelli Mine lek.NC_MM_bams.tgzPony Express bamsThis file contains the sorted .bam files for individuals from the Pony Express lek.PE_PE_bams.tgzDome House bamsThis file contains the sorted .bam files for individuals from the Dome House lek.PV_DH_bams.tgzGable Canyon bamsThis file contains the sorted .bam files for individuals from the Gable Canyon lek.PV_GC_bams.tgzPinefield bamsThis file contains the sorted .bam files for individuals from the Pinefield lek.PV_PF_bams.tgzBig Pole bamsThis file contains the sorted .bam files for individuals from the Big Pole lek.NC_BP_bams.tgz