cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21676662
title: Substitution of PINK1 Gly411 modulates substrate receptivity and turnover

description: The ubiquitin (Ub) kinase-ligase pair PINK1-PRKN mediates the degradation of damaged mitochondria by macroautophagy/autophagy (mitophagy). PINK1 surveils mitochondria and upon stress accumulates on the mitochondrial surface where it phosphorylates serine 65 of Ub to activate PRKN and to drive mitochondrial turnover. While loss of either PINK1 or PRKN is genetically linked to Parkinson disease (PD) and activating the pathway seems to have great therapeutic potential, there is no formal proof that stimulation of mitophagy is always beneficial. Here we used biochemical and cell biological methods to study single nucleotide variants in the activation loop of PINK1 to modulate the enzymatic function of this kinase. Structural modeling and <i>in vitro</i> kinase assays were used to investigate the molecular mechanism of the PINK1 variants. In contrast to the PD-linked PINK1<sup>G411S</sup> mutation that diminishes Ub kinase activity, we found that the PINK1<sup>G411A</sup> variant significantly boosted Ub phosphorylation beyond levels of PINK1 wild type. This resulted in augmented PRKN activation, mitophagy rates and increased viability after mitochondrial stress in midbrain-derived, gene-edited neurons. Mechanistically, the G411A variant stabilizes the kinase fold of PINK1 and transforms Ub to adopt the preferred, C-terminally retracted conformation for improved substrate turnover. In summary, we identify a critical role of residue 411 for substrate receptivity that may now be exploited for drug discovery to increase the enzymatic function of PINK1. The genetic substitution of Gly411 to Ala increases mitophagy and may be useful to confirm neuroprotection <i>in vivo</i> and might serve as a critical positive control during therapeutic development. <b>Abbreviations</b>: ATP: adenosine triphosphate; CCCP: carbonyl cyanide m-chlorophenyl hydrazone; Ub-CR: ubiquitin with C-terminally retracted tail; CTD: C-terminal domain (of PINK1); ELISA: enzyme-linked immunosorbent assay; HCI: high-content imaging; IB: immunoblot; IF: immunofluorescence; NPC: neuronal precursor cells; MDS: molecular dynamics simulation; PD: Parkinson disease; p-S65-Ub: ubiquitin phosphorylated at Ser65; RMSF: root mean scare fluctuation; TOMM: translocase of outer mitochondrial membrane; TVLN: ubiquitin with T66V and L67N mutation, mimics Ub-CR; Ub: ubiquitin; WT: wild-type.
DOI: 10.6084/m9.figshare.21676662.v1
title: Substitution of PINK1 Gly411 modulates substrate receptivity and turnover

description: The ubiquitin (Ub) kinase-ligase pair PINK1-PRKN mediates the degradation of damaged mitochondria by macroautophagy/autophagy (mitophagy). PINK1 surveils mitochondria and upon stress accumulates on the mitochondrial surface where it phosphorylates serine 65 of Ub to activate PRKN and to drive mitochondrial turnover. While loss of either PINK1 or PRKN is genetically linked to Parkinson disease (PD) and activating the pathway seems to have great therapeutic potential, there is no formal proof that stimulation of mitophagy is always beneficial. Here we used biochemical and cell biological methods to study single nucleotide variants in the activation loop of PINK1 to modulate the enzymatic function of this kinase. Structural modeling and <i>in vitro</i> kinase assays were used to investigate the molecular mechanism of the PINK1 variants. In contrast to the PD-linked PINK1<sup>G411S</sup> mutation that diminishes Ub kinase activity, we found that the PINK1<sup>G411A</sup> variant significantly boosted Ub phosphorylation beyond levels of PINK1 wild type. This resulted in augmented PRKN activation, mitophagy rates and increased viability after mitochondrial stress in midbrain-derived, gene-edited neurons. Mechanistically, the G411A variant stabilizes the kinase fold of PINK1 and transforms Ub to adopt the preferred, C-terminally retracted conformation for improved substrate turnover. In summary, we identify a critical role of residue 411 for substrate receptivity that may now be exploited for drug discovery to increase the enzymatic function of PINK1. The genetic substitution of Gly411 to Ala increases mitophagy and may be useful to confirm neuroprotection <i>in vivo</i> and might serve as a critical positive control during therapeutic development. <b>Abbreviations</b>: ATP: adenosine triphosphate; CCCP: carbonyl cyanide m-chlorophenyl hydrazone; Ub-CR: ubiquitin with C-terminally retracted tail; CTD: C-terminal domain (of PINK1); ELISA: enzyme-linked immunosorbent assay; HCI: high-content imaging; IB: immunoblot; IF: immunofluorescence; NPC: neuronal precursor cells; MDS: molecular dynamics simulation; PD: Parkinson disease; p-S65-Ub: ubiquitin phosphorylated at Ser65; RMSF: root mean scare fluctuation; TOMM: translocase of outer mitochondrial membrane; TVLN: ubiquitin with T66V and L67N mutation, mimics Ub-CR; Ub: ubiquitin; WT: wild-type.
DOI: 10.6084/m9.figshare.21676662.v2
title: Substitution of PINK1 Gly411 modulates substrate receptivity and turnover

description: The ubiquitin (Ub) kinase-ligase pair PINK1-PRKN mediates the degradation of damaged mitochondria by macroautophagy/autophagy (mitophagy). PINK1 surveils mitochondria and upon stress accumulates on the mitochondrial surface where it phosphorylates serine 65 of Ub to activate PRKN and to drive mitochondrial turnover. While loss of either PINK1 or PRKN is genetically linked to Parkinson disease (PD) and activating the pathway seems to have great therapeutic potential, there is no formal proof that stimulation of mitophagy is always beneficial. Here we used biochemical and cell biological methods to study single nucleotide variants in the activation loop of PINK1 to modulate the enzymatic function of this kinase. Structural modeling and <i>in vitro</i> kinase assays were used to investigate the molecular mechanism of the PINK1 variants. In contrast to the PD-linked PINK1<sup>G411S</sup> mutation that diminishes Ub kinase activity, we found that the PINK1<sup>G411A</sup> variant significantly boosted Ub phosphorylation beyond levels of PINK1 wild type. This resulted in augmented PRKN activation, mitophagy rates and increased viability after mitochondrial stress in midbrain-derived, gene-edited neurons. Mechanistically, the G411A variant stabilizes the kinase fold of PINK1 and transforms Ub to adopt the preferred, C-terminally retracted conformation for improved substrate turnover. In summary, we identify a critical role of residue 411 for substrate receptivity that may now be exploited for drug discovery to increase the enzymatic function of PINK1. The genetic substitution of Gly411 to Ala increases mitophagy and may be useful to confirm neuroprotection <i>in vivo</i> and might serve as a critical positive control during therapeutic development. <b>Abbreviations</b>: ATP: adenosine triphosphate; CCCP: carbonyl cyanide m-chlorophenyl hydrazone; Ub-CR: ubiquitin with C-terminally retracted tail; CTD: C-terminal domain (of PINK1); ELISA: enzyme-linked immunosorbent assay; HCI: high-content imaging; IB: immunoblot; IF: immunofluorescence; NPC: neuronal precursor cells; MDS: molecular dynamics simulation; PD: Parkinson disease; p-S65-Ub: ubiquitin phosphorylated at Ser65; RMSF: root mean scare fluctuation; TOMM: translocase of outer mitochondrial membrane; TVLN: ubiquitin with T66V and L67N mutation, mimics Ub-CR; Ub: ubiquitin; WT: wild-type.
DOI: 10.5281/zenodo.7980346
title: IS network seismograms from the Mw7.8 and Mw7.5 2023 Kahramanmaraş earthquakes

description: M7.?.tar.gz : TAR+GZIP files containing 10 minutes of seismic data from the IS network recording the 2023 Kahramanmaraş earthquake doublet ????_??.tar.gz: TAR+GZIP files of selected IS stations in the Northern Dead Sea basin between 01/2021 to 03/2023. local_eq.tar.gz: TAR+GZIP files of IS stations in the Jordan Valley containing local earthquake data
DOI: 10.5281/zenodo.7980345
title: IS network seismograms from the Mw7.8 and Mw7.5 2023 Kahramanmaraş earthquakes

description: M7.?.tar.gz : TAR+GZIP files containing 10 minutes of seismic data from the IS network recording the 2023 Kahramanmaraş earthquake doublet ????_??.tar.gz: TAR+GZIP files of selected IS stations in the Northern Dead Sea basin between 01/2021 to 03/2023. local_eq.tar.gz: TAR+GZIP files of IS stations in the Jordan Valley containing local earthquake data
DOI: 10.34740/kaggle/dsv/5908580
title: Egypt Gold Prices | Daily Updated
DOI: 10.18712/nsd-nsd0964-v1
title: KEU Customer impact surveys 1996

description: When the Statens Nærings- og Distriktutviklingsfond (SND) was established, emphasis was placed on developing a target and result reporting system that could make the new institution develop in a uniform manner. After thorough work, a system was implemented in 1994 to measure both effects, activity and productivity. In principle, this system included all the instruments managed by SND. For each cohort, a pre-survey is carried out one year after the service was provided, in addition to a follow-up survey four years later. For the operational year 1996, Møreforsking Molde carried out an evaluation survey in 1997, as well as a follow-up survey in 2000.

The survey covers the following areas:

General information about the project for which support is sought
The establishment's organization and implementation
The significance of support from SND for strategy, competence and the like
Results and effects of the support from SND
General information about SND
Background information
SND was established on the basis of the Storting's processing of the Government's legislative proposal in Ot.prp. no. 57 (1991-92) and established with effect from 3 July 1992. At the same time as SND was established, the previous institutions Distriketenes Utbyggsfond, Småbedriftsfondet and Industrifondet were closed down and their operations taken up in the new special law company. From the same time, Industribanken AS was dissolved.
DOI: 10.18712/nsd-nsd0965-v1
title: KEU Customer impact surveys 1998

description: When the Statens Nærings- og Distriktutviklingsfond (SND) was established, emphasis was placed on developing a target and result reporting system that could make the new institution develop in a uniform manner. After thorough work, a system was implemented in 1994 to measure both effects, activity and productivity. In principle, this system included all the instruments managed by SND. For each cohort, a pre-survey is carried out one year after the service was provided, in addition to a follow-up survey four years later. For the operational year 1998, Møreforsking Molde carried out an evaluation survey in 1999, as well as a follow-up survey in 2002.

The survey covers the following areas:

1. General information about the project for which support is sought
2. The establishment's organization and implementation
3. The significance of support from SND for strategy, competence and the like
4. Results and effects of the support from SND
5. General information about SND
6. Background information

SND was established on the basis of the Storting's processing of the Government's legislative proposal in Ot.prp. no. 57 (1991-92) and established with effect from 3 July 1992. At the same time as SND was established, the previous institutions Distriketenes Utbyggsfond, Småbedriftsfondet and Industrifondet were closed down and their operations taken up in the new special law company. 5. From the same time, Industribanken AS was dissolved.
DOI: 10.6084/m9.figshare.23501316
title: The data of the article "<strong>Development and validation of the artificial intelligence learning intention scale (AILIS) for university students</strong>"

description: As artificial intelligence (AI) permeates almost all aspects of our lives, university students need to acquire relevant knowledge, skills, and attitudes to adapt to the challenges it poses. This study involved the development and validation of a scale, called the Artificial Intelligence Learning Intention Scale (AILIS), which measured the relevant factors that shape university students’ behavioral intentions to learn about AI. We recruited 907 Chinese university students who answered the survey. The scale is comprised of 9 factors that are categorized into various dimensions pertaining to the epistemic capacity (AI basic knowledge, programming efficacy, designing AI for social good), facilitating environment (actual use of AI systems, subjective norms, access to support and technology), psychological attitudes (resilience, optimism, personal relevance), and outcomes (behavioral intention to learn AI, actual learning of AI). Reliability analyses and confirmatory factor analyses indicated that the scale has acceptable reliability and construct validity. In addition, structural equational modelling found that 16 out of the 17 hypotheses tested were supported. Overall, the findings reveal that Chinese university students express a strong intention to learn about AI, and this behavioral intention is closely associated with actual learning. We also found several key factors associated with students’ behavioral intention to learn AI. The study contextualizes the theory of planned behavior for university AI education, provides guidelines on the design of AI curriculum courses, and means of assessing course effectiveness.
DOI: 10.6084/m9.figshare.23501256
title: Kuhn_et_al_2023_JVS_traits_clean_20200323.xlsx

description: This is the raw dataset generated during the study, Kühn <em>et al.</em> (2023), "Root trait variation along water gradients in the Cape Floristic Region" <em>J. Veg. Sci.</em> Raw data is presented in the first sheet and column descriptions and units are presented in the second sheet.
DOI: 10.6084/m9.figshare.23501256.v1
title: Kuhn_et_al_2023_JVS_traits_clean_20200323.xlsx

description: This is the raw dataset generated during the study, Kühn <em>et al.</em> (2023), "Root trait variation along water gradients in the Cape Floristic Region" <em>J. Veg. Sci.</em> Raw data is presented in the first sheet and column descriptions and units are presented in the second sheet.
DOI: 10.5517/ccdc.csd.cc2dg8rx
title: CCDC 2218259: Experimental Crystal Structure Determination

description: Related Article: Y. Avila, O. Pérez, L. Sánchez, M. C. Vázquez, R. Mojica, M. González, M. Ávila, J. Rodríguez-Hernández, E. Reguera|2023|New J.Chem.|47|10781|doi:10.1039/D3NJ01498C
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2dg8sy
title: CCDC 2218260: Experimental Crystal Structure Determination

description: Related Article: Y. Avila, O. Pérez, L. Sánchez, M. C. Vázquez, R. Mojica, M. González, M. Ávila, J. Rodríguez-Hernández, E. Reguera|2023|New J.Chem.|47|10781|doi:10.1039/D3NJ01498C
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2dg8tz
title: CCDC 2218261: Experimental Crystal Structure Determination

description: Related Article: Y. Avila, O. Pérez, L. Sánchez, M. C. Vázquez, R. Mojica, M. González, M. Ávila, J. Rodríguez-Hernández, E. Reguera|2023|New J.Chem.|47|10781|doi:10.1039/D3NJ01498C
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.6084/m9.figshare.23501319
title: Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD canine model of Duchenne Muscular Dystrophy

description: Raw data for muscle physiology and fibre typing in the natural history study of the DE50-MD canine model of DMD. <br> Twitch and tetani - raw data include: Twitch and tetani all data Twitch and tetani analysed data only Twitch and tetanus ratio data <br> Eccentric contraction - raw data includes: Eccentric contraction protocol data Linear reg of age and eccentric contraction decrement Linear red of max absolute force and eccentric contraction decrement <br> Fibre typing - raw data includes: IHC fibre typing results IHC fibre typing raw images RT-qPCR fibre typing normalised values RT-qPCR fibre typing relative values
DOI: 10.5281/zenodo.7472749
title: DATASET: A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters

description: Dataset related to "<strong>A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters"</strong> <strong>Structure:</strong> - Data: contains the data to be used to create the results. - Results: contains the results for each crater. Code available here: <pre>https://doi.org/10.5281/zenodo.7472818</pre>
DOI: 10.5281/zenodo.7473178
title: DATASET: A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters

description: Dataset related to "A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters" Structure: - Data: this folder contains the data used in our work (imagery, digital elevation model, and slope map)<br> - Results: this folder contains the resulting faults, transects, plots, and spreadsheets, presented in our work <br> Code available here: https://doi.org/10.5281/zenodo.7472818<br>
DOI: 10.5281/zenodo.7472748
title: DATASET: A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters

description: Dataset related to "A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters" Structure: - Data: this folder contains the data used in our work (imagery, digital elevation model, and slope map)<br> - Results: this folder contains the resulting faults, transects GIS files and spreadsheets presented in our work <br> Code available here: <pre>https://doi.org/10.5281/zenodo.7477125</pre>
DOI: 10.5281/zenodo.7503302
title: DATASET: A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters

description: Dataset related to "A semi-automated analysis of displacement-to-length scaling of the grabens affecting lunar Floor-Fractured craters" Structure: - Data: this folder contains the data used in our work (imagery, digital elevation model, and slope map)<br> - Results: this folder contains the resulting faults, transects GIS files and spreadsheets presented in our work <br> Code available here: <pre>https://doi.org/10.5281/zenodo.7477125</pre>
DOI: 10.11588/data/yyleku
title: Stable Carbon Isotope Signature of Methane Released from Phytoplankton [Research Data]

description: Aquatic ecosystems play an important role in global methane cycling and many field studies have reported methane supersaturation in the oxic surface mixed layer (SML) of the ocean and in the epilimnion of lakes. The origin of methane formed under oxic condition is hotly debated and several pathways have recently been offered to explain the ‘methane paradox’. In this context, stable isotope measurements have been applied to constrain methane sources in supersaturated oxygenated waters. Here we present stable carbon isotope signatures for six widespread marine phytoplankton species, three haptophyte algae and three cyanobacteria, incubated under laboratory conditions. The observed isotopic patterns implicate that methane formed by phytoplankton might be clearly distinguished from methane produced by methanogenic archaea. Comparing results from phytoplankton experiments with isotopic data from field measurements, suggests that algal and cyanobacterial populations may contribute substantially to methane formation observed in the SML of oceans and lakes.
DOI: 10.11588/data/yyleku/8sfbti
title: Data_to_Isotopic_Patterns_of_Methane_Production_from_Phytoplankton.tab

description: :unav
DOI: 10.26300/4ce5-jk07
title: Data for Vasculature and resident macrophage are present in the zebrafish gonad prior to sex determination
DOI: 10.48758/ufz.12355
title: Eddy covariance rawdata for Hohes Holz (DE-HoH) in Central Germany, 49m for 202003

description: Eddy-covariance Rawdata (20Hz) from CSAT and LI7500 Hohes Holz 49m, March 2020. Converted from slt- and dia-files to ascii-format (u, v, w, T_Sonic, CO2, H2O, p_75, SA_DIAG, GA75_DIAG), no time column, individual file names are indicating the starting time of the data acquisition period (according to RFlux-requirements)

Hohes Holz DoY61-91 (Mar 2020)

file: DE-HoH_202003_eddy-raw_csv.zip
more files available in the same repository
DOI: 10.5281/zenodo.7156656
title: Brondata: Evaluatie archeologie 2016-2021. Kenniswinst archeologieregelgeving.

description: In deze zenodo-record zijn de brongegevens terug te vinden waarop de analyses in het hoofdstuk "Kenniswinst archeologieregelgeving 2016-2021" zijn gebaseerd. Dit hoofdstuk maakt deel uit van het onderzoeksrapport "Ameels V, Carpentier F, De Ketelaere S, Ervynck A, Geuens J, Haneca K, Pieters M, Van Looveren J & Verhelst A 2023: Evaluatie archeologie 2016-2021, Onderzoeksrapport Agentschap Onroerend Erfgoed, Brussel.": <strong>1. Een overzicht van alle ingediende eindverslagen (n = 576) en nota's met eindafwerking (n = 26) over de periode 1 april 2016 t.e.m. 31 december 2021</strong> eindverslagen_notas_2016_2021_overzicht.csv (<em>comma-separated values</em>) eindverslagen_notas_2016_2021_overzicht.xlsx (<em>Microsoft Excel Open XML Spreadsheet</em>) eindverslagen_notas_2016_2021_overzicht.shp (<em>ESRI shapefile</em>) (Een overzicht van alle ingediende eindverslagen - vanaf 1 april 2016 tot vandaag - kan je ook downloaden als GIS-bestand op https://geo.onroerenderfgoed.be/downloads.) <strong>2. De resultaten van de inhoudelijke screening (periodisering, materiaalcategorieën, ...) van deze documenten:</strong> eindverslagen_notas_2016_2021_screening.csv (<em>comma-separated values</em>) eindverslagen_notas_2016_2021_screening.xlsx (<em>Microsoft Excel Open XML Spreadsheet</em>) eindverslagen_notas_2016_2021_screening.shp (<em>ESRI shapefile</em>)