DOI: 10.5061/dryad.p5hqbzkmw
title: Microsatellite genotypes of Aedes japonicus collected in Belgium and Germany

description: Seven polymorphic microsatellite loci were scored in 292 specimens of Ae.
 japonicus collected in Belgium and Germany to investigate the population
 genetic structure of this invasive. The objectives of the study
 were to determine (i) if the mosquito specimens collected along the
 Belgian border were introduced from the nearby existing West German
 population, and (ii) if the population at Natoye (Belgium) resulted from a
 new introduction event and/or from a few undetected specimens that escaped
 elimination.
description: <em>Aedes japonicus</em> specimens were collected in
 the framework of different successive projects undertaken to monitor the
 introduction and establishment of exotic mosquito species in Belgium and
 Germany. DNA was extracted from legs, abdomens or eggs using either the
 NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel) or the QIAamp DNA
 Micro kit (Qiagen). Specimens were genotyped for seven microsatellite loci
 developed for <em>Ae. japonicus</em>, using the two
 multiplexes as presented in Widdel et al. 2005 (sized on a 3130XL Genetic
 Analyzer, using GeneScanTM 500 LIZ as size standard). Microsatellite
 length variation visualization and determination was performed using
 Geneious® R11.
description: GENERAL INFORMATION<br> 1. Title of Dataset: <br>
 MICROSATELLITE GENOTYPES OF AEDES JAPONICUS COLLECTED IN BELGIUM AND
 GERMANY 2. Author Information     A.
 Principal Investigator Contact Information       
  Name: NATHALIE SMITZ<br>         Institution: ROYAL MUSEUM FOR
 CENTRAL AFRICA<br>         Address: LEUVENSESTEENWEG 17 3080
 TERVUREN BELGIUM<br>         Email:
 NATHALIE.SMITZ@AFRICAMUSEUM.BE     B. Associate or
 Co-investigator Contact Information         Name: WIM
 VAN BORTEL<br>         Institution: OUTBREAK RESEARCH TEAM,
 INSTITUTE OF TROPICAL MEDICINE<br>         Address: NATIONALESTRAAT
 155 2000 ANTWERP BELGIUM<br>         Email:
 WVANBORTEL@ITG.BE     C. Alternate Contact
 Information         Name: DEBLAUWE ISRA<br>   
      Institution: OUTBREAK RESEARCH TEAM, INSTITUTE OF TROPICAL
 MEDICINE<br>         Address: NATIONALESTRAAT 155 2000 ANTWERP
 BELGIUM<br>         Email: IDEBLAUWE@ITG.BE 3.
 Date of data collection (single date, range, approximate date): 
 AEDES JAPONICUS SPECIMENS WERE COLLECTED FROM 2012 UNTIL 2019,
 DURING EXOTIC MOSQUITO MONITORING PROGRAMS.<br> MICROSATELLITE
 GENOTYPES WERE GENERATED IN 2020. 4. Geographic
 location of data collection:  BELGIUM AND GERMANY:
 EXACT LOCATION DETAILS ARE DISPLAYED IN THE EXCEL SHEET FOR EACH PROCESSED
 SPECIMEN.<br> LABPRATORY MANIPULATIONS WERE REALISED IN THE ROYAL
 MUSEUM FOR CENTRAL AFRICA. 5. Information about funding
 sources that supported the collection of the data: 
 This work in Belgium is part of the MEMO project, funded by the
 Flemish, Walloon and Brussels regional governments and the Federal Public
 Service (FPS) Public Health, Food Chain Safety and Environment in the
 context of the National Environment and Health Action Plan (NEHAP)
 (Belgium). <br> The Barcoding Facility for Organisms and Tissues of
 Policy Concern (BopCo - http://bopco.myspecies.info/) is financed by the
 Belgian Science Policy Office (Belspo) as Belgian federal in-kind
 contribution to the European Research Infrastructure Consortium
 “LifeWatch”. <br> The Outbreak Research Team of the Institute of
 Tropical Medicine is funded by the Department of Economy, Science and
 Innovation of the Flemish government. SHARING/ACCESS
 INFORMATION 1. Was data derived from another
 source?  NO 2. Recommended citation
 for this dataset:  PLEASE CITE THE PUBLICATION OF SMITZ
 ET AL., IN WHICH THE DRYAD DOI IS CITED FIRST. DATA
 & FILE OVERVIEW 1. File List: 
 Smitz et al. Aedes_japonicus_microsatellite_genotypes.xls: EXCEL
 SHEET WITH THE MICROSATELLITE GENOTYPES FOR EACH PROCESSED SPECIMEN,
 INCLUDING COLLECTION LOCATION AND YEAR. 2. Additional
 related data collected that was not included in the current data
 package:  NAD4 FRAGMENT WAS ALSO SCORED IN 278
 SPECIMENS AND DEPOSITED IN GENBANK WITH ACCESSION NUMBERS: MT462702 -
 MT462979. 3. Are there multiple versions of the
 dataset? NO METHODOLOGICAL
 INFORMATION<br> 1. Description of methods used for
 collection/generation of data:  Aedes japonicus
 specimens were collected in the framework of different successive projects
 undertaken to monitor the introduction and establishment of exotic
 mosquito species in Belgium and Germany.<br> DNA was extracted from
 legs, abdomens or eggs using either the NucleoSpin® Tissue DNA extraction
 kit (Macherey-Nagel) or the QIAamp DNA Micro kit (Qiagen).
 2. Methods for processing the data:  Specimens
 were genotyped for seven microsatellite loci developed for Ae. japonicus,
 using the two multiplexes presented in Widdel et al. 2005.
 3. Instrument- or software-specific information needed to
 interpret the data:  Microsatellite length variation
 visualization and determination was performed using Geneious®
 R11. 4. People involved with sample collection,
 processing, analysis and/or submission:  Additional
 collaborators are: Katrien De Wolf, Helge Kampen, Francis Schaffner,
 Jacobus De Witte, Anna Schneider, Ingrid Verlé, Adwine Vanslembrouck,
 Wouter Dekoninck, Kenny Meganck, Sophie Gombeer, Ann Vanderheyden, Marc De
 Meyer, Thierry Backeljau, Doreen Werner, Ruth Müller
 DATA-SPECIFIC INFORMATION FOR: Smitz et al.
 Aedes_japonicus_microsatellite_genotypes.xls 1. Number
 of variables: 12 2. Number of rows: 586 (diploid
 organism-&gt; two rows per individual) 3. Variable
 List:  Column A: "Sample Code" - code
 attribuated to each specimen<br> Column B: "Country" -
 either Germany or Belgium, Europe<br> Column C: "State /
 Province" - State where the specimen was collected in Germany, and
 province where the specimen was collected in Belgium<br> Column D:
 "Locality" - name of the city where the specimen was
 collected<br> Column E: "Collection year" - year of
 collection (2012 until 2019)<br> Column F - L: "Microsatellite
 genotypes" - size of each microsatellite loci (N = 7), sized on a
 3130XL Genetic Analyzer, using GeneScanTM 500 LIZ as size
 standard 4. Missing data code: -9