DOI: 10.5061/dryad.15dv41p59
title: Integration of attractive and defensive phytochemicals is unlikely to constrain chemical diversification in a perennial herb

description: Diversification of plant chemical phenotypes is typically associated with
 spatially and temporally variable plant-insect interactions. Floral scent
 is often assumed to be the target of pollinator-mediated selection,
 whereas foliar compounds are considered targets of antagonist-mediated
 selection. However, floral and vegetative phytochemicals can be
 biosynthetically linked and may thus evolve as integrated
 phenotypes. Utilizing a common garden of 28 populations of the
 perennial herb Arabis alpina (Brassicaceae), we investigated
 integration within and among floral scent compounds and foliar defense
 compounds (both volatile compounds and tissue-bound glucosinolates).
 Within floral scent volatiles, foliar volatile compounds, and
 glucosinolates, phytochemicals were often positively correlated, and
 correlations were stronger within these groups than between them. Thus, we
 found no evidence of integration between compound groups indicating that
 these are free to evolve independently. Relative to self-compatible
 populations, self-incompatible populations experienced stronger
 correlations between floral scent compounds and a trend towards lower
 integration between floral scent and foliar volatiles. Our study serves as
 a rare test of the integration of multiple, physiologically related plant
 traits that each are potential targets of insect-mediated selection. Our
 results suggest that independent evolutionary forces are likely to
 diversify different axes of plant chemistry without major constraints.
description: This data was collected in 2020 and 2021 at Lund university,
 Sweden and ETH Zürich, Switzerland.  The data has been
 processed and cleaned for publication according to standard practices in
 the field of phytochemistry. Details on data handling can be found in the
 associated manuscript and supporting information.
description: # Integration of attractive and defensive phytochemicals is unlikely to
 constrain chemical diversification in a perennial herb
 [https://doi.org/10.5061/dryad.15dv41p59](https://doi.org/10.5061/dryad.15dv41p59) The two tables included in this repository contains information on floral scent, foliar volatile and glucosinolate content for 28 populations of the perennial herb *Arabis alpina* L. (Brassicaceae). Detailed descriptions of methods can be found in the associated publication. ## Description of the data and file structure The data is structured in **two Excel files**. Each file contains two sheets, one README, and one datasheet. All compounds are reported in standardized units as either nanograms/flower/hour (floral scent volatiles), nanograms/gram/hour (foliar volatiles), or μmol g-1 dry tissue weight (glucosinolates). Floral and foliar volatiles were collected using dynamic headspace and subsequent GC/MS analysis, and glucosinolates were analysed using LC/MS. This data can be used for emission rate analysis and compound content analysis, appropriate for among-population variation. ## Code/Software All packages used to analyze this data for publication are accessible in R (v. 4.3.1) and R Studio (v. 2023.06.2). Information on the *bouquet* package used to produce the floral and foliar volatile data is available here: Eisen KE, Powers JM, Raguso RA and Campbell DR (2022) An analytical pipeline to support robust research on the ecology, evolution, and function of floral volatiles. Front. Ecol. Evol. 10:1006416. doi: 10.3389/fevo.2022.1006416