DOI: 10.57760/sciencedb.j00217.10496
title: XU Jin1,2, LI Ke1,2, ZHANG Xiaoxia3, MA Junbai1, WANG Hao1 1. School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004,China; 2. Ningxia Hui Autonomous Region People's Hospital, Yinchuan 750002; 3.College of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan 750004, China.
title: Clostridium sporogenes 对Graves 病小鼠髓源抑制性细胞及炎症因子的影响

description: This study was designed to analyze the effects of Clostridium sporogenes (C. sporogenes) on myeloid-derived suppressor cells (MDSCs) and inflammatory factors in mice with Graves disease (GD).A GD mice model was constructed using recombinant adenovirus overexpressing Ad-hTSHR-289 in 6-week-old female BALB/C mice.After modeling, the mice were randomly divided into 6 groups,(8 mice/group): blank control group (CON), C. sporogenes intervention negative control group (CON_C.sp), C. sporogenes supernatant intervention negative control group (CON_C.sp_supernate), GD model group (MOD), C. sporogenes intervention GD model group (MOD_C.sp) and C. sporogenes supernatant intervention GD model group (MOD_ C.sp_supernate), and gave normal saline (NS), C. sporogenes, and C. sporogenes supernatant gavage intervention for 6 weeks, the proportions of MDSCs and their subtype monocyte-like myeloid-derived suppressor cells M-MDSCs in spleen were respectively detected by flow cytometry. The expression levels of tumor necrosis factor -α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), free thyroxine (FT4), thyroid-stimulating hormone receptor antibody (TRAb) and thyroid-stimulating hormone (TSH) in plasma were determined by ELISA.Pearson method was used to analyze the correlation between MDSCs , M-MDSCs , inflammatory cytokines and thyroxine. Compared with the model group mice, the proportion of M-MDSCs in GD model group (MOD_C.sp_supernate) treated with C. sporogenes supernatant was increased in spleen. The plasma TNF-α and IL-6 of the GD model group (MOD_C.sp and MOD_C.sp_supernate) treated with C. sporogenes and their supernatant were decreased, and IL-10 was increased. Analysis of the correlation between Spleen MDSCs ,M-MDSCs and plasma inflammatory cytokines showed that Spleen MDSCs and M-MDSCs were negatively correlated with plasma TNF-α and IL-6,, but positively correlated with IL-10. Spleen MDSCs and M-MDSCs were negatively correlated with plasma FT4 and TRAb, but positively correlated with TSH .In conclusion, C. sporogenes can increase the proportion of M-MDSCs in the spleen of GD mice, and improve GD and inflammatory response in mice.
description: This study was designed to analyze the effects of Clostridium sporogenes (C. sporogenes) on myeloid-derived suppressor cells (MDSCs) and inflammatory factors in mice with Graves disease (GD).A GD mice model was constructed using recombinant adenovirus overexpressing Ad-hTSHR-289 in 6-week-old female BALB/C mice.After modeling, the mice were randomly divided into 6 groups,(8 mice/group): blank control group (CON), C. sporogenes intervention negative control group (CON_C.sp), C. sporogenes supernatant intervention negative control group (CON_C.sp_supernate), GD model group (MOD), C. sporogenes intervention GD model group (MOD_C.sp) and C. sporogenes supernatant intervention GD model group (MOD_ C.sp_supernate), and gave normal saline (NS), C. sporogenes, and C. sporogenes supernatant gavage intervention for 6 weeks, the proportions of MDSCs and their subtype monocyte-like myeloid-derived suppressor cells M-MDSCs in spleen were respectively detected by flow cytometry. The expression levels of tumor necrosis factor -α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), free thyroxine (FT4), thyroid-stimulating hormone receptor antibody (TRAb) and thyroid-stimulating hormone (TSH) in plasma were determined by ELISA.Pearson method was used to analyze the correlation between MDSCs , M-MDSCs , inflammatory cytokines and thyroxine. Compared with the model group mice, the proportion of M-MDSCs in GD model group (MOD_C.sp_supernate) treated with C. sporogenes supernatant was increased in spleen. The plasma TNF-α and IL-6 of the GD model group (MOD_C.sp and MOD_C.sp_supernate) treated with C. sporogenes and their supernatant were decreased, and IL-10 was increased. Analysis of the correlation between Spleen MDSCs ,M-MDSCs and plasma inflammatory cytokines showed that Spleen MDSCs and M-MDSCs were negatively correlated with plasma TNF-α and IL-6,, but positively correlated with IL-10. Spleen MDSCs and M-MDSCs were negatively correlated with plasma FT4 and TRAb, but positively correlated with TSH .In conclusion, C. sporogenes can increase the proportion of M-MDSCs in the spleen of GD mice, and improve GD and inflammatory response in mice.