cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9956864
title: Nutritional value of non-conventional vegetables prepared by family farmers in rural communities

description: ABSTRACT: Four most consumed non-conventional vegetables were analyzed raw and after cooking techniques routinely used by family farmers: ora-pro-nobis (Pereskia aculeata Mill.); wild mustard (Sinapis arvensis L.), serralha (Sonchus arvensis L), and capiçova (Erechtites valeriana). Chemical composition was determined according to AOAC. Vitamin C, vitamin E and carotenoids were determined by high-performance liquid chromatography, and phenolic compounds and minerals by spectrophotometry. Vitamin E and carotenoids concentrations were higher in stir fried wild mustard (7.68 mg.100 g-1 and 7.45 mg.100 g-1, respectively). Cooking reduced some minerals concentration in the non-conventional vegetables, but increased vitamins and carotenoids concentrations. The vegetables presented high content of minerals but low protein concentration and total energy content. Non-conventional vegetables can be considered of excellent nutritional value and frequent consumption of these vegetables can contribute to improve the feeding of farmers and their families.
DOI: 10.6084/m9.figshare.9956879.v1
title: DNA hypermethylation of Fgf16 and Tbx22 associated with cleft palate during palatal fusion

description: Abstract Objective: Cleft palate (CP) is a congenital birth defect caused by the failure of palatal fusion. Little is known about the potential role of DNA methylation in the pathogenesis of CP. This study aimed to explore the potential role of DNA methylation in the mechanism of CP. Methodology: We established an all-trans retinoic acid (ATRA)-induced CP model in C57BL/6J mice and used methylation-dependent restriction enzymes (MethylRAD, FspEI) combined with high-throughput sequencing (HiSeq X Ten) to compare genome-wide DNA methylation profiles of embryonic mouse palatal tissues, between embryos from ATRA-treated vs. untreated mice, at embryonic gestation day 14.5 (E14.5) (n=3 per group). To confirm differentially methylated levels of susceptible genes, real-time quantitative PCR (qPCR) was used to correlate expression of differentially methylated genes related to CP. Results: We identified 196 differentially methylated genes, including 17,298 differentially methylated CCGG sites between ATRA-treated vs. untreated embryonic mouse palatal tissues (P&lt;0.05, log2FC&gt;1). The CP-related genes Fgf16 (P=0.008, log2FC=1.13) and Tbx22 (P=0.011, log2FC=1.64,) were hypermethylated. Analysis of Fgf16 and Tbx22, using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), identified 3 GO terms and 1 KEGG pathway functionally related to palatal fusion. The qPCR showed that changes in expression level negatively correlated with methylation levels. Conclusions: Taken together, these results suggest that hypermethylation of Fgf16 and Tbx22 is associated with decreased gene expression, which might be responsible for developmental failure of palatal fusion, eventually resulting in the formation of CP.
DOI: 10.6084/m9.figshare.9956861
title: AEROBIC CAPACITY OF CHILEAN ADULTS AND ELDERLY: PROPOSAL OF CLASSIFICATION BY REGIONAL PERCENTILES

description: ABSTRACT Introduction Aerobic fitness is an important predictor that contributes to the preservation of functional independence during the aging process. Its measurement represents a fundamental tool in the identification of multiple health problems. Objective To compare the aerobic capacity of adults and elderly subjects through international studies and to develop percentiles by age group using the LMS method. Methods A cross-sectional descriptive study was conducted with 1146 subjects (437 men and 709 women). The age group of the sample ranged from 50 to 84 years. The subjects evaluated came from the physical activity programs offered by the National Sports Institute (IND) and by the city council of Talca (Chile). Body mass, stature, oxygen saturation (SatO2), six-minute walk test, and systolic and diastolic blood pressure were assessed. Body Mass Index (BMI) was calculated for both sexes. The LMS method was used to propose the percent distribution. Results Aerobic capacity decreases with age (28.5% for men and 29.9% for women). There was a negative relationship between age and the six-minute walk test (men r = -0.13 and women r = -0.39). There was a discrepancy between the elderly subjects in the current study and those from international studies. The normative data for the classification of aerobic fitness were expressed in percentiles (p3, p5, p10, p15, p25, p50, p75, p85, p90, p95 and p97). Conclusion The aerobic performance of elderly subjects diminishes as they age; in addition, the current results differ from international studies, which motivated the development of percentiles to classify aerobic fitness in everyday situations, especially in places with few resources and particularly where field tests are considered a priority for large-scale physical evaluation. Level of evidence II; Diagnostic studies – investigation of diagnostic test.
DOI: 10.6084/m9.figshare.9956864.v1
title: Nutritional value of non-conventional vegetables prepared by family farmers in rural communities

description: ABSTRACT: Four most consumed non-conventional vegetables were analyzed raw and after cooking techniques routinely used by family farmers: ora-pro-nobis (Pereskia aculeata Mill.); wild mustard (Sinapis arvensis L.), serralha (Sonchus arvensis L), and capiçova (Erechtites valeriana). Chemical composition was determined according to AOAC. Vitamin C, vitamin E and carotenoids were determined by high-performance liquid chromatography, and phenolic compounds and minerals by spectrophotometry. Vitamin E and carotenoids concentrations were higher in stir fried wild mustard (7.68 mg.100 g-1 and 7.45 mg.100 g-1, respectively). Cooking reduced some minerals concentration in the non-conventional vegetables, but increased vitamins and carotenoids concentrations. The vegetables presented high content of minerals but low protein concentration and total energy content. Non-conventional vegetables can be considered of excellent nutritional value and frequent consumption of these vegetables can contribute to improve the feeding of farmers and their families.
DOI: 10.6084/m9.figshare.9956867.v1
title: Aquatic vascular plants of South Brazil: checklist and a comparative floristic approach

description: ABSTRACT Aquatic ecosystems support species diversity, and knowledge of plant communities is essential for wetland conservation programs. This study provides a checklist of aquatic vascular plants of South Brazil and establishes their floristic affinities with bordering South America phytogeographical domains. The checklist was based on 52 sources, including 35 floristic lists, 17 regional taxonomic studies, and information from an electronic database on wetlands of South Brazil. Floristic similarities with published checklists for neighbouring regions were assessed. A total of 780 species distributed in 277 genera and 85 families of vascular plants were reported for South Brazil. Families with higher species richness were Cyperaceae (128), Poaceae (102), Asteraceae (69), Plantaginaceae (21), Lentibulariaceae (20), and Onagraceae (20). The most represented genera were Eleocharis (41) and Cyperus (24). A cluster analysis revealed high similarity with Iberá (Argentinian Chaco), Pampa and Atlantic Forest, and low similarity with Pantanal, Caatinga and the Amazon Rainforest. The high number of aquatic plant families recorded relative to temperate and tropical climates suggests a zone of biogeographical overlap in Southern Brazil. The compiled data set reveals high biodiversity of wetlands of South Brazil, provides a baseline for future research, and highlights the need for regional conservation planning.
DOI: 10.6084/m9.figshare.9956870.v1
title: EFFECT OF 16 WEEKS OF RESISTANCE TRAINING ON STRENGTH ENDURANCE IN MEN AND WOMEN

description: ABSTRACT Introduction Although resistance training (RT) can provide numerous benefits for both men and women, morphological, neuromuscular, metabolic, physiological, and behavioral differences between sexes may influence the magnitude of training responses. Objective To analyze the impact of 16 weeks of progressive RT on strength endurance in untrained men and women. Methods Twenty-eight men and 31 women (18-30 years) underwent a supervised RT program that was divided into two 8-week stages, 3 times per week on nonconsecutive days. The RT program was composed of exercises for different body segments (trunk, upper and lower limbs) that were performed with three sets of 8-12 repetitions maximum (RM), in 10 and 12 exercises, in the first and second stage, respectively. Strength endurance was assessed in 3 exercises (bench press, squat, and arm curl) and in a combination of these exercises through a protocol composed of 4 sets performed to failure with 80% of 1-RM on the baseline, after 8 and 16 weeks of RT. Results Group vs. time interactions (p &lt;0.05) were found for bench press (men = +28.3% vs. women = +32.1%), squat (men = +13.5% vs. women = +32.7%), and arm curl (men = +20.2% vs. women = +24.4%) exercises, as well as in the set of all 3 exercises (men = +18.4% vs. women = +31.2%). Conclusion Our results suggest that 16 weeks of RT can improve strength endurance in both men and women, although higher gains are achieved by women. Level of evidence II; Therapeutic study-Investigating treatment results.
DOI: 10.6084/m9.figshare.9956849
title: Contents of total phenolics and flavonoids in and antioxidant activity of Ilex paraguariensis leaves

description: Abstract: The objective of this work was to evaluate different solvents and times in the ultrasound-assisted extraction of polyphenols, in order to quantify the total phenolic and flavonoid contents in and the antioxidant activity of mate (Ilex paraguariensis) leaves. The experiment was carried out in a completely randomized design, in a 8x3 (solvent x time of extraction) factorial arrangement. The solvent extractors were distilled water and aqueous solutions of ethanol, acetone, and methanol, acidified or not with hydrochloric acid, and the time of ultrasound-assisted extractions were 15, 30, and 45 min. All extractions were finished with a mechanical agitation of 30 min. Samples treated with acidified hydroalcoholic solvents resulted in higher values of total phenolics and flavonoids and antioxidant activity. The extraction using acidified hydroethanol solvent was not affected by the time in the ultrasound. The contents of total phenolics and flavonoids were highly correlated with each other and with the antioxidant activity, especially when it was determined by the ferric-reducing antioxidant power method. Acidified hydroethanol solvent in ultrasound for 15 min is the most suitable extraction condition for the quantification of the contents of total phenolics and flavonoids in and the antioxidant activity of mate leaves.
DOI: 10.6084/m9.figshare.9956852
title: RFID in building: proposal of a system model for organizing maintenance information

description: Abstract The construction industry is seen as a knowledge-based industry. Throughout the process of designing a building, much information is generated, and it is written down in a series of documents that, upon completion of the work, must be delivered to the owners and managers of the buildings for the proper maintenance of the property. This information must be organised in such a way as to enable it to be retrieved and used where necessary. The use of new information and communication technologies (ICTs) helps in this organisation. These include the Radio-Frequency Identification (RFID) technology, an automated data collection technology that have already been adopted by some industries. This paper aims to propose a computerised system model for accessing maintenance information, using RFID tags installed in building elements, mobile reading devices and free software in order to reduce the costs of implementation and operation of the system. An experiment was carried out in order to validate the proposed model, as well as to demonstrate the contribution of the model to the improvement of access to information. This paper contributes to the discussion on the application of RFID technology associated with mobile devices to building maintenance.
DOI: 10.6084/m9.figshare.9956855
title: Homophobic discrimination through humor: naturalization and heteronormativity maintenance in the organizational context

description: Abstract This research is part of the studies on sexual diversity in organizations, focusing on the theme of homophobic humor. The aim was to investigate whether homophobic humor is present in the workplace and in what way. In theoretical terms, guided by an interpretativist approach, the article is based on studies on discrimination by means of homophobia and humor, as well as research on heteronormativity in organizations. In order to fulfill the objective, a content analysis was carried out to analyze the interviews carried out with homosexual, bisexual and heterosexual employees of a company. A case study was conducted in a company interviewing employees, regardless their sexual orientation. The interviews were analyzed using Bardin’s content analysis method. The presence of the following was observed: frequent homophobic humor; naturalized homophobic humor; homophobic jokes as speech to maintain heteronormativity. There is a scenario in which it is necessary to take actions that promote diversity, especially in relation to the issue of humor, as well as regarding the lack of understanding on the part of workers that jokes containing homophobic humor are linked to oppression. This article contributes to the deepening of national studies on discrimination based on sexual orientation, specifically regarding homophobic humor, and also in terms of relating this type of discrimination to the theme of heteronormativity in the organizational context through the concepts of queer theory.
DOI: 10.6084/m9.figshare.9956858
title: Interproscan annotation results of proteins in Chlorella vulgaris NJ-7

description: Interproscan annotation results of proteins in Chlorella vulgaris NJ-7
DOI: 10.6084/m9.figshare.9956861.v1
title: AEROBIC CAPACITY OF CHILEAN ADULTS AND ELDERLY: PROPOSAL OF CLASSIFICATION BY REGIONAL PERCENTILES

description: ABSTRACT Introduction Aerobic fitness is an important predictor that contributes to the preservation of functional independence during the aging process. Its measurement represents a fundamental tool in the identification of multiple health problems. Objective To compare the aerobic capacity of adults and elderly subjects through international studies and to develop percentiles by age group using the LMS method. Methods A cross-sectional descriptive study was conducted with 1146 subjects (437 men and 709 women). The age group of the sample ranged from 50 to 84 years. The subjects evaluated came from the physical activity programs offered by the National Sports Institute (IND) and by the city council of Talca (Chile). Body mass, stature, oxygen saturation (SatO2), six-minute walk test, and systolic and diastolic blood pressure were assessed. Body Mass Index (BMI) was calculated for both sexes. The LMS method was used to propose the percent distribution. Results Aerobic capacity decreases with age (28.5% for men and 29.9% for women). There was a negative relationship between age and the six-minute walk test (men r = -0.13 and women r = -0.39). There was a discrepancy between the elderly subjects in the current study and those from international studies. The normative data for the classification of aerobic fitness were expressed in percentiles (p3, p5, p10, p15, p25, p50, p75, p85, p90, p95 and p97). Conclusion The aerobic performance of elderly subjects diminishes as they age; in addition, the current results differ from international studies, which motivated the development of percentiles to classify aerobic fitness in everyday situations, especially in places with few resources and particularly where field tests are considered a priority for large-scale physical evaluation. Level of evidence II; Diagnostic studies – investigation of diagnostic test.
DOI: 10.6084/m9.figshare.9956849.v1
title: Contents of total phenolics and flavonoids in and antioxidant activity of Ilex paraguariensis leaves

description: Abstract: The objective of this work was to evaluate different solvents and times in the ultrasound-assisted extraction of polyphenols, in order to quantify the total phenolic and flavonoid contents in and the antioxidant activity of mate (Ilex paraguariensis) leaves. The experiment was carried out in a completely randomized design, in a 8x3 (solvent x time of extraction) factorial arrangement. The solvent extractors were distilled water and aqueous solutions of ethanol, acetone, and methanol, acidified or not with hydrochloric acid, and the time of ultrasound-assisted extractions were 15, 30, and 45 min. All extractions were finished with a mechanical agitation of 30 min. Samples treated with acidified hydroalcoholic solvents resulted in higher values of total phenolics and flavonoids and antioxidant activity. The extraction using acidified hydroethanol solvent was not affected by the time in the ultrasound. The contents of total phenolics and flavonoids were highly correlated with each other and with the antioxidant activity, especially when it was determined by the ferric-reducing antioxidant power method. Acidified hydroethanol solvent in ultrasound for 15 min is the most suitable extraction condition for the quantification of the contents of total phenolics and flavonoids in and the antioxidant activity of mate leaves.
DOI: 10.6084/m9.figshare.9956855.v1
title: Homophobic discrimination through humor: naturalization and heteronormativity maintenance in the organizational context

description: Abstract This research is part of the studies on sexual diversity in organizations, focusing on the theme of homophobic humor. The aim was to investigate whether homophobic humor is present in the workplace and in what way. In theoretical terms, guided by an interpretativist approach, the article is based on studies on discrimination by means of homophobia and humor, as well as research on heteronormativity in organizations. In order to fulfill the objective, a content analysis was carried out to analyze the interviews carried out with homosexual, bisexual and heterosexual employees of a company. A case study was conducted in a company interviewing employees, regardless their sexual orientation. The interviews were analyzed using Bardin’s content analysis method. The presence of the following was observed: frequent homophobic humor; naturalized homophobic humor; homophobic jokes as speech to maintain heteronormativity. There is a scenario in which it is necessary to take actions that promote diversity, especially in relation to the issue of humor, as well as regarding the lack of understanding on the part of workers that jokes containing homophobic humor are linked to oppression. This article contributes to the deepening of national studies on discrimination based on sexual orientation, specifically regarding homophobic humor, and also in terms of relating this type of discrimination to the theme of heteronormativity in the organizational context through the concepts of queer theory.
DOI: 10.6084/m9.figshare.9956852.v1
title: RFID in building: proposal of a system model for organizing maintenance information

description: Abstract The construction industry is seen as a knowledge-based industry. Throughout the process of designing a building, much information is generated, and it is written down in a series of documents that, upon completion of the work, must be delivered to the owners and managers of the buildings for the proper maintenance of the property. This information must be organised in such a way as to enable it to be retrieved and used where necessary. The use of new information and communication technologies (ICTs) helps in this organisation. These include the Radio-Frequency Identification (RFID) technology, an automated data collection technology that have already been adopted by some industries. This paper aims to propose a computerised system model for accessing maintenance information, using RFID tags installed in building elements, mobile reading devices and free software in order to reduce the costs of implementation and operation of the system. An experiment was carried out in order to validate the proposed model, as well as to demonstrate the contribution of the model to the improvement of access to information. This paper contributes to the discussion on the application of RFID technology associated with mobile devices to building maintenance.
DOI: 10.6084/m9.figshare.9956840
title: Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells

description: Abstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation.