DOI: 10.5061/dryad.1pj04
title: Data from: Enhanced diversity and aflatoxigenicity in interspecific hybrids of Aspergillus flavus and Aspergillus parasiticus

description: Aspergillus flavus and A. parasiticus are the two most important
 aflatoxin-producing fungi responsible for the contamination of
 agricultural commodities worldwide. Both species are heterothallic and
 undergo sexual reproduction in laboratory crosses. Here we examine the
 possibility of interspecific matings between A. flavus and A. parasiticus.
 These species can be distinguished morphologically and genetically, as
 well as by their mycotoxin profiles. Aspergillus flavus produces both B
 aflatoxins and cyclopiazonic acid (CPA), B aflatoxins or CPA alone, or
 neither mycotoxin; Aspergillus parasiticus produces B and G aflatoxins or
 the aflatoxin precursor O-methylsterigmatocystin, but not CPA. Only four
 of forty-five attempted interspecific crosses between opposite mating
 types of A. flavus and A. parasiticus were fertile and produced viable
 ascospores. Single ascospore strains from each cross were shown to be
 recombinant hybrids using multilocus genotyping and array comparative
 genome hybridization. Conidia of parents and their hybrid progeny were
 haploid and predominantly monokaryons and dikaryons based on flow
 cytometry. Multilocus phylogenetic inference showed that experimental
 hybrid progeny were grouped with naturally occurring A. flavus L strain
 and A. parasiticus. Higher total aflatoxin concentrations in some F1
 progeny strains compared to midpoint parent aflatoxin levels indicate
 synergism in aflatoxin production; moreover, three progeny strains
 synthesized G aflatoxins that were not produced by the parents, and there
 was evidence of allopolyploidization in one strain. These results suggest
 that hybridization is an important diversifying force resulting in the
 genesis of novel toxin profiles in these agriculturally important fungi.
description: CEL file for isolate
 IC899aCGH raw data submitted
 to Gene Expression Omnibus001143_3357.CELCEL file for isolate IC904aCGH raw data submitted to Gene Expression Omnibus001154_RIB40_020907.CELCEL file for isolate IC158aCGH raw data submitted to Gene Expression Omnibus001447_NRRL1957_DNA.CELCEL file for isolate IC277aCGH raw data submitted to Gene Expression Omnibus001448_IC277_DNA.CELCEL file for isolate IC903aCGH raw data submitted to Gene Expression Omnibus001523_RIB128.CELCEL file for isolate IC902aCGH raw data submitted to Gene Expression Omnibus001524_RIB430.CELCEL file for isolate IC1aCGH raw data submitted to Gene Expression Omnibus001857_IC-1.CELCEL file for isolate IC33aCGH raw data submitted to Gene Expression Omnibus001858_IC-33.CELCEL file for isolate IC75aCGH raw data submitted to Gene Expression Omnibus001859_IC-75.CELCEL file for isolate IC1410aCGH raw data submitted to Gene Expression Omnibus001860_IC-1410.CELCEL file for isolate IC313aCGH raw data submitted to Gene Expression OmnibusEA08090_81339_AFLAVUSa520391F_IC313.CELCEL file for isolate IC201aCGH raw data submitted to Gene Expression OmnibusEA08090_82453_AFLAVUSa520391F_IC201.CELCEL file for isolate IC311aCGH raw data submitted to Gene Expression OmnibusEA08090_82455_AFLAVUSa520391F_IC311.CELCEL file for isolate IC278aCGH raw data submitted to Gene Expression OmnibusEA08090_89554_AFLAVUSa520391F_278.CELCEL file for isolate IC1612aCGH raw data submitted to Gene Expression OmnibusEA08090_89555_AFLAVUSa520391F_1612.CELCEL file for isolate IC1616aCGH raw data submitted to Gene Expression OmnibusEA08090_89556_AFLAVUSa520391F_1616.CELCEL file for isolate IC65aCGH raw data submitted to Gene Expression OmnibusEA08090_90215_AFLAVUSa520391F_65.CELCEL file for isolate IC1622aCGH raw data submitted to Gene Expression OmnibusEA08090_90216_AFLAVUSa520391F_1622.CELCEL file for isolate IC308aCGH raw data submitted to Gene Expression OmnibusEA08090_90217_AFLAVUSa520391F_308.CELCEL file for isolate IC1637aCGH raw data submitted to Gene Expression OmnibusEA08090_90218_AFLAVUSa520391F_1637.CELCEL file for isolate IC35aCGH raw data submitted to Gene Expression OmnibusEA08090_90797_AFLAVUSa520391F_35.CELCEL file for isolate IC36aCGH raw data submitted to Gene Expression OmnibusEA08090_90798_AFLAVUSa520391F_36.CELCEL file for isolate IC307aCGH raw data submitted to Gene Expression OmnibusEA08090_90799_AFLAVUSa520391F_307.CELCEL file for isolate IC1719aCGH raw data submitted to Gene Expression OmnibusEA08090_90800_AFLAVUSa520391F_1719.CELCEL file for isolate IC1722aCGH raw data submitted to Gene Expression OmnibusEA08090_90801_AFLAVUSa520391F_1722.CELCEL file for isolate IC1751aCGH raw data submitted to Gene Expression OmnibusEA08090_90802_AFLAVUSa520391F_1751.CELCEL file for isolate IC310aCGH raw data submitted to Gene Expression OmnibusEA08090_91004_AFLAVUSa520391F_310.CELCEL file for isolate IC31aCGH raw data submitted to Gene Expression OmnibusEA08090_91005_AFLAVUSa520391F_31.CELCEL file for isolate IC244aCGH raw data submitted to Gene Expression OmnibusEA08090_91006_AFLAVUSa520391F_244.CELCEL file for isolate IC2207aCGH raw data submitted to Gene Expression OmnibusEA08090_91007_AFLAVUSa520391F_2207.CELCEL file for isolate IC327aCGH raw data submitted to Gene Expression OmnibusEA08090_91153_AFLAVUSa520391F_327.CELCEL file for isolate IC1179aCGH raw data submitted to Gene Expression OmnibusEA08090_91154_AFLAVUSa520391F_1179.CELCEL file for isolate IC1603aCGH raw data submitted to Gene Expression OmnibusEA08090_91155_AFLAVUSa520391F_1603.CELCEL file for isolate IC1650aCGH raw data submitted to Gene Expression OmnibusEA08090_91156_AFLAVUSa520391F_1650.CELCEL file for isolate IC2205aCGH raw data submitted to Gene Expression OmnibusEA08090_91157_AFLAVUSa520391F_2205.CELCEL file for isolate IC2209aCGH raw data submitted to Gene Expression OmnibusEA08090_91158_AFLAVUSa520391F_2209.CELCEL file for isolate IC640aCGH raw data submitted to Gene Expression OmnibusEA08090_91862_AFLAVUSa520391F_640.CELCEL file for isolate IC1566aCGH raw data submitted to Gene Expression OmnibusEA08090_91863_AFLAVUSa520391F_1566.CELCEL file for isolate IC1533aCGH raw data submitted to Gene Expression OmnibusEA08090_92106_AFLAVUSa520391F_1533.CELCEL file for isolate IC324aCGH raw data submitted to Gene Expression OmnibusEA08090_93860_AFLAVUSa520391F_324.CELCEL file for isolate IC1630aCGH raw data submitted to Gene Expression OmnibusEA08090_93861_AFLAVUSa520391F_1630.CELCEL file for isolate IC1112aCGH raw data submitted to Gene Expression OmnibusEA08090_96998_AFLAVUSa520391F_1112.CELCEL file for isolate IC1516aCGH raw data submitted to Gene Expression OmnibusEA08090_96999_AFLAVUSa520391F_1516.CELCEL file for isolate IC1766aCGH raw data submitted to Gene Expression OmnibusEA08090_99115_AFLAVUSa520391F_1766.CELCEL file for isolate IC2171aCGH raw data submitted to Gene Expression OmnibusEA08090_99117_AFLAVUSa520391F_2171.CELCEL file for isolate IC1755aCGH raw data submitted to Gene Expression OmnibusEA08090_99815_AFLAVUSa520391F_1775.CELCEL file for isolate IC301aCGH raw data submitted to Gene Expression OmnibusEA08090_99816_AFLAVUSa520391F_301.CELCEL file for isolate IC34aCGH raw data submitted to Gene Expression OmnibusEA08090_99817_AFLAVUSa520391F_34_2.CELCEL file for isolate IC720aCGH raw data submitted to Gene Expression OmnibusEA08090_109845_AFLAVUSa520391F_720.CELCEL file for isolate IC796aCGH raw data submitted to Gene Expression OmnibusEA08090_109846_AFLAVUSa520391F_796.CELCEL file for isolate IC1142aCGH raw data submitted to Gene Expression OmnibusEA08090_109847_AFLAVUSa520391F_1142.CELCEL file for isolate IC1169aCGH raw data submitted to Gene Expression OmnibusEA08090_109848_AFLAVUSa520391F_1169.CELCEL file for isolate IC3655aCGH raw data submitted to Gene Expression OmnibusEA08090_115513_AFLAVUSA520391F_1C3655.CELCEL file for isolate IC3656aCGH raw data submitted to Gene Expression OmnibusEA08090_115514_AFLAVUSA520391F_1C3656.CELCEL file for isolate IC3657aCGH raw data submitted to Gene Expression OmnibusEA08090_115515_AFLAVUSA520391F_1C3657.CELCEL file for isolate IC4047aCGH raw data submitted to Gene Expression OmnibusEA08090_130747_AFLAVUSA520391F_IC4047.CELFigure 4 - aCGH data for dendrogramdendrogram_data_log_transfored_unnormalized_09302014.xlsbFigure 5 - aCGH data for heat mapfigure5_heatmap_data_log_transformed_loess_normalized_09302014.xlsbaCGH metadataaCGH metadata submitted to Gene Expression OmnibusOLARTE_metadata_updated_9.30.14.xlsaflM_aflN locus - A. parasiticusBankit submission to GenBankSubmission1628464.txtaflW_aflX locus - A. parasiticusBankit submission to GenBankSubmission1628476.txttrpC locus - A. parasiticusBankit submission to GenBankSubmission1628485.txtaflC_aflD locus - A. parasiticusBankit submission to GenBankSubmission1633431.txtaflC_aflD locus - A. parasiticus x. A. flavusBankit submission to GenBankSubmission1633434.txtaflM_aflN locus - A. parasiticus x. A. flavusBankit submission to GenBankSubmission1633435.txtaflW_aflX locus - A. parasiticus x. A. flavusBankit submission to GenBankSubmission1633437.txttrpC locus - A. parasiticus x. A. flavusBankit submission to GenBankSubmission1633443.txtFigure S1 - Flow CytometryRaw data for Figure S1flow cytometry.xlsxTable 1Raw data for Table 1 including: number of sclerotia/stromata per slant, number of sclerotia/stromata examined, percent with ascocarps, and percent with ascospore-bearing ascocarps.Table 2 - Colony DiameterTable 2 Colony dia.xlsxTable 2 - Conidial DiameterTable 2 Conidial dia.xlsxTable 2 - Sclerotial WeightTable 2 Sclerotial weight.xlsxTable 2 - Stipe LengthTable 2 Stipe length.xlsxTable 3 - CMLST_alignment_278x33DNA sequence alignment for cluster multilocus sequence typing of 278x33 crossCMLST_alignment_278x33.nxsTable 3 - CMLST_alignment_278x65DNA sequence alignment for cluster multilocus sequence typing of 278x65 crossCMLST_alignment_278x65.nxsTable 3 - CMLST_alignment_278x324DNA sequence alignment for cluster multilocus sequence typing of 278x324 crossCMLST_alignment_278x324.nxsTable 3 - CMLST_alignment_278x327DNA sequence alignment for cluster multilocus sequence typing of 278x327 crossCMLST_alignment_278x327.nxsTable 3 - GMLST_alignment_278x33DNA sequence alignment for genome multilocus sequence typing of 278x33 crossGMLST_alignment_278x33.nxsTable 3 - GMLST_alignment_278x65DNA sequence alignment for genome multilocus sequence typing of 278x65 crossGMLST_alignment_278x65.nxsTable 3 - GMLST_alignment_278x324DNA sequence alignment for genome multilocus sequence typing of 278x324 crossGMLST_alignment_278x324.nxsTable 3 - GMLST_alignment_278x327DNA sequence alignment for genome multilocus sequence typing of 278x327 crossGMLST_alignment_278x327.nxsTable 3 - mycotoxinsHPLC data for mycotoxinsTable 3 Final mycotoxins.xlsxFigure 6 - Global B aflatoxin isolatesGlobal_Bs.csvFigure 6 - Global G aflatoxin isolatesGlobal_Gs.csvFigure 6 - Global locality fileglobal_locality_iTOL_NEW.csvFigure 6 - Global species fileglobal_species_iTOL_NEW.csvFigure 6 - RAxML fileRAxML_bipartitionsBranchLabels.MLST_3_lociFigure 6 - RAxML multiple sequence alignment filecombine_intersect.nxs