cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/f53aa-9tk54
title: Bdellovibrio/Vibrio cholerae

description: Bdellovibrio invasion complex with Vibrio cholerae MKW1383Janelia liquid nitrogen auto-filling system failed in the middle of data collection
description: <strong>Tilt Series Date:</strong> 2013-06-04</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 40 min</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-04-11/rawdata/</p>atlas_29x29_20011.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-04-11/rawdata/atlas_29x29_20011.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_29x29_20011_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 215.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-04-11/3dimage_27027/atlas_29x29_20011_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wxdq8-7pb12
title: Skinny E. coli with GFP-MreB

description: Raw data files of Skinny E. coli with GFP-MreB
description: <strong>Tilt Series Date:</strong> 2010-03-05</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR and Peach</p>
<strong>Collaborators and Roles:</strong> Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)</p>
<strong>Sample Preparation:</strong> R2/2 CF or Quantifoil, 10 nm gold, 100% humidity</p>
  zla2010-03-05-32_slicer9793.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-32/rawdata/</p>10mar05a_ZL001_032.mrc,  Tilt Series (Pixel Size 1.192 nm), 721.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-32/rawdata/10mar05a_ZL001_032.mrc"
        > <i class="download icon"></i> Download </a></p>

        10mar05a_ZL001_032_part.rec,  Reconstruction (Pixel Size 2.384 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-32/3dimage_9345/10mar05a_ZL001_032_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hm1qd-59z19
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-03-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-07-8_slicer8220.jpg: Filment bundle in Myxococcus Xanthus ycw2014-03-07-8_slicer8224.jpg: Filaments in Myxococcus Xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-07-8/rawdata/</p>20140306_YWC092_10007.mrc,  Tilt Series (Pixel Size 4.2 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-07-8/rawdata/20140306_YWC092_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140306_YWC092_10007_full.rec,  Reconstruction (Pixel Size 16.8 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-07-8/3dimage_39797/20140306_YWC092_10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bmyx5-zjc62
title: Hylemonella gracilis

description: flagellar motor, storage granule, weird cytosolic density
description: <strong>Tilt Series Date:</strong> 2012-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 112.0 eV/Å²,  defocus: -15.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2012-09-23-44_slicer5103.jpg: Filament ycw2012-09-23-44_slicer5105.jpg: Filament ycw2012-09-23-44_slicer5106.jpg: Filament ycw2012-09-23-44_slicer5109.jpg: Flagellar Motors ycw2012-09-23-44_slicer5110.jpg: Unknown ycw2012-09-23-44_slicer5112.jpg: Filament ycw2012-09-23-44_slicer5113.jpg: Unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-44/rawdata/</p>20120923_Hylemonella_30008.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-44/rawdata/20120923_Hylemonella_30008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120923_Hylemonella_30008_full.rec,  Reconstruction (Pixel Size 1.96 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-44/3dimage_20940/20120923_Hylemonella_30008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zmbt2-zz258
title: Hylemonella gracilis

description: nucleoid filament, flagellar motor, chemoreceptor array
description: <strong>Tilt Series Date:</strong> 2012-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 112.0 eV/Å²,  defocus: -15.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2012-09-23-42_slicer5083.jpg: Chemoreceptor array ycw2012-09-23-42_slicer5084.jpg: Flagellar motor (two) ycw2012-09-23-42_slicer5087.jpg: Unknown ycw2012-09-23-42_slicer5089.jpg: Peptidoglycan ycw2012-09-23-42_slicer5093.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-42/rawdata/</p>20120923_Hylemonella_30006.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-42/rawdata/20120923_Hylemonella_30006.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120923_Hylemonella_30006_full.rec,  Reconstruction (Pixel Size 1.96 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-42/3dimage_20936/20120923_Hylemonella_30006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/71mtq-zxd69
title: AimB depletion control w/ xylose

description: Raw data files of AimB depletion control w/ xylose
description: <strong>Tilt Series Date:</strong> 2009-09-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach, IMOD</p>
<strong>Collaborators and Roles:</strong> The AimB_depletion strain was constructed by John Werner, data collected by ZL</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity</p>
  zla2009-09-21-18_slicer10167.jpg: Caulobacter crescentus .  flat vesicle zla2009-09-21-18_slicer10170.jpg: Caulobacter crescentus .  flat vesicle zla2009-09-21-18_slicer10171.jpg: Caulobacter crescentus .  flat vesicle zla2009-09-21-18_slicer10172.jpg: Caulobacter crescentus .  filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-18/rawdata/</p>09sep21a_AimBxyl_036.mrc,  Tilt Series (Pixel Size 1.192 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-18/rawdata/09sep21a_AimBxyl_036.mrc"
        > <i class="download icon"></i> Download </a></p>

        09sep21a_AimBxyl_036_full.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-09-21-18/3dimage_10350/09sep21a_AimBxyl_036_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gcpkk-cq273
title: Myxococcus xanthus TM530

description: Wza0 knockoutGrid box YWC029
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Tam Mignot</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-10-25-18_slicer9076.jpg: ring .  Myxococcus xanthus ycw2013-10-25-18_slicer9077.jpg: filament .  Myxococcus xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-18/rawdata/</p>20131025_YWC029_10002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-18/rawdata/20131025_YWC029_10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_YWC029_10002_part121_50.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-18/3dimage_33374/20131025_YWC029_10002_part121_50.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.he2ush
title: Occurrence Download

description: A dataset containing 2592699 species occurrences available in GBIF matching the query:
{
 "DatasetKey" : [
 "is Pl@ntNet observations"
 ]
}

 The dataset includes 2592699 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013613-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/g571n-45t83
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-12-19</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, place the tube on bench for ~20 min, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~20 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-58/3dimage_71668/</p>20151219_YWC206_10058_part121_2.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-58/3dimage_71668/20151219_YWC206_10058_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        20151219_YWC206_10058.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-58/rawdata/20151219_YWC206_10058.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nne2w-7pr58
title: Agro_AD1408_GFP-VirB11

description: AD1408: pAdi16/A348dB11; Kanamycin 50; Functional GFP-VirB11 fusion.
description: <strong>Tilt Series Date:</strong> 2015-07-21</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 121.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow culture in AB medium (pH 7) at 30C. For induction, use 375 µl of a decent ON culture to inoculate 5 ml AB mes pH 5.8 medium containing 100 µM Acetosyringone and antibiotic (Kan 50). Grow at 20C for 18 hr. Freeze cells.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  dga2015-07-21-3_slicer14030.jpg: Regularly spread periplasmic  space.
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-07-21-3/rawdata/</p>1408_atlas0003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-07-21-3/rawdata/1408_atlas0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        1408_atlas0003_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-07-21-3/3dimage_59348/1408_atlas0003_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.gh9jeq
title: Occurrence Download

description: A dataset containing 1728 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Paspalum mandiocanum",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1728 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013627-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ntr55-9h495
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-09-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2017-09-29-5_slicer18884.jpg: cytoplasmic vesicles dga2017-09-29-5_slicer18885.jpg: many extracellular vesicular extentions from the outer membrane
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-29-5/rawdata/</p>Cb_d5pH4_201709290016.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-29-5/rawdata/Cb_d5pH4_201709290016.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_d5pH4_201709290016_part61_24.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
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        > <i class="download icon"></i> Download </a></p>