cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/c5hvt-d3j26
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-21/rawdata/</p>20160424_YWC212_20011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-21/rawdata/20160424_YWC212_20011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20011_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-21/3dimage_74844/20160424_YWC212_20011_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20011_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-21/3dimage_74845/20160424_YWC212_20011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xjds9-14t24
title: HeLa cells expressing TRIM5α-YFP

description: HeLa cell line 25 expressing stable TRIM5α-YFP. Correlated lightelectron microscopy (11062015Tom). Concentration of 500 nmblue beads (phosphorex) 4/150. 20 nm gold highly concentrated.Manual blotting with Whatman 40.HeLa cell line 25 expressingstable TRIM5α-YFP.
description: <strong>Tilt Series Date:</strong> 2015-10-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cell line 25 and VSV-G pseudotyped virus Vpr-Tomato provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells infected with pseudovirus Vpr-Tomato (in box). 500 µl of media and 500 µl of virus. Incubated with MG-132 (Campbell et al 2008) 6hrs. Cells were washed with warm media and incubated at 37°C before freezing.</p>
<strong>Sample Preparation:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-11/3dimage_71018/</p>tomography3.rec,  Reconstruction (Pixel Size 24.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-11/3dimage_71018/tomography3.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography3.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-11/rawdata/tomography3.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography3_full.rec,  Reconstruction (Pixel Size 0.0 nm), 533.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-11/3dimage_76102/tomography3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.nsu4yq
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus sideroxylon",
 "Country is Mozambique",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045040-250811113504898/datasets/export for details.
DOI: 10.22002/nqhzm-a5w58
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-24/rawdata/</p>20160424_YWC212_20014.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-24/rawdata/20160424_YWC212_20014.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20014_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-24/3dimage_74836/20160424_YWC212_20014_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20014_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-24/3dimage_74837/20160424_YWC212_20014_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a4bwgb
title: Occurrence Download

description: A dataset containing 3 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus sideroxylon",
 "Country is Portugal",
 "HasCoordinate is true"
 ]
}

 The dataset includes 3 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045042-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/yfqtg-pjn20
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-67/3dimage_73323/</p>20150511_AK07_10067_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-67/3dimage_73323/20150511_AK07_10067_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10067.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-67/rawdata/20150511_AK07_10067.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10067_SIRT_2K.rec~,  5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-67/file_16624/20150511_AK07_10067_SIRT_2K.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bcaws-6x206
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-71/3dimage_73326/</p>20150511_AK07_10071_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-71/3dimage_73326/20150511_AK07_10071_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10071.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-71/rawdata/20150511_AK07_10071.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10071_SIRT_2K.rec~,  5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-71/file_16627/20150511_AK07_10071_SIRT_2K.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mr2ae-7f470
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-19/rawdata/</p>20160424_YWC212_20009.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-19/rawdata/20160424_YWC212_20009.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20009_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-19/3dimage_74834/20160424_YWC212_20009_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20009_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-19/3dimage_74835/20160424_YWC212_20009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g8pmj-wvy53
title: Rhesus Macaque Fibroblast

description: Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate any virus because light image only taken in the red channel.
description: <strong>Tilt Series Date:</strong> 2015-04-20</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-04-20-1/rawdata/</p>tomography1.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-04-20-1/rawdata/tomography1.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography1_full.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-04-20-1/3dimage_54606/tomography1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography1_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-04-20-1/3dimage_55711/tomography1_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.vy3z8v
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus sideroxylon",
 "Country is Pakistan",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045041-250811113504898/datasets/export for details.
DOI: 10.22002/6dvf2-jec48
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-59/rawdata/</p>exsD-mini-cell_target_2.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-59/rawdata/exsD-mini-cell_target_2.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_2_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-59/3dimage_105528/exsD-mini-cell_target_2_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_2.mrc.mdoc,  63.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-59/rawdata/exsD-mini-cell_target_2.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/edrey-nd112
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-33/rawdata/</p>exsD-mini-cell_target_19.mrc,  Tilt Series (Pixel Size 0.45 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-33/rawdata/exsD-mini-cell_target_19.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_19_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-33/3dimage_105374/exsD-mini-cell_target_19_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_19.mrc.mdoc,  20.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-33/rawdata/exsD-mini-cell_target_19.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>