cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.79zmv9
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Copadichromis diplostigma, Copadichromis geertsi, Copadichromis ilesi, Copadichromis insularis, Copadichromis jacksoni, Copadichromis likomae, Copadichromis mbenjii, Copadichromis melas, Copadichromis mloto, Copadichromis nkatae, Copadichromis parvus, Copadichromis pleurostigma, Copadichromis pleurostigmoides, Copadichromis quadrimaculatus, Copadichromis trewavasae, Copadichromis trimaculatus, Copadichromis verduyni, Copadichromis virginalis, Coptodon bakossiorum, Coptodon bemini, Coptodon bythobates, Coptodon cameronensis, Coptodon camerunensis, Coptodon coffea, Coptodon congica, Coptodon dageti, Coptodon deckerti, Coptodon discolor, Coptodon ejagham, Coptodon flava, Coptodon fusiforme, Coptodon guineensis, Coptodon gutturosa, Coptodon imbriferna, Coptodon ismailiaensis, Coptodon kottae, Coptodon louka, Coptodon margaritacea, Coptodon nigrans, Coptodon nyongana, Coptodon rendalli, Coptodon rheophila, Coptodon snyderae, Coptodon spongotroktis, Coptodon tholloni, Coptodon thysi, Coptodon walteri, Coptodon zillii, Coptostomabarbus bellcrossi, Coptostomabarbus wittei, Corematodus shiranus, Corematodus taeniatus, Coryogalops william, Cotylopus acutipinnis, Cotylopus rubripinnis, Crenimugil buchanani, Crenimugil crenilabis, Crenimugil seheli, Cromeria nilotica, Cromeria occidentalis, Ctenochromis horei, Ctenochromis luluae, Ctenochromis oligacanthus, Ctenochromis polli, Ctenogobius lepturus, Ctenopharyngodon idella, Ctenopharynx intermedius, Ctenopharynx nitidus, Ctenopharynx pictus, Ctenopoma acutirostre, Ctenopoma ashbysmithi, Ctenopoma kingsleyae, Ctenopoma machadoi, Ctenopoma multispine, Ctenopoma maculatum, Ctenopoma muriei, Ctenopoma nebulosum, Ctenopoma nigropannosum, Ctenopoma ocellatum, Ctenopoma petherici, Ctenopoma weeksii, Cunningtonia longiventralis, Cyathochromis obliquidens, Cyathopharynx furcifer, Cyclopharynx fwae, Cyclopharynx schwetzi, Cynotilapia afra, Cynotilapia aurifrons, Cynotilapia axelrodi, Cynotilapia chilundu, Cynotilapia pulpican, Cynotilapia zebroides, Microchromis zebroides, Cyphomyrus discorhynchus, Cyphomyrus macrops, Hippopotamyrus macrops, Cyphomyrus psittacus, Hippopotamyrus psittacus, Cyphomyrus wilverthi, Hippopotamyrus wilverthi)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((59.3519 -17.3042,59.9568 -17.101,60.2121 -16.521,59.9717 -15.9401,59.2192 -15.9401,58.9855 -16.8095,59.1248 -17.1702,59.3519 -17.3042))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007980-250827131500795/datasets/export for details.
DOI: 10.22002/61bxw-ae520
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-45/3dimage_73311/</p>20150511_AK07_10045_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-45/3dimage_73311/20150511_AK07_10045_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10045.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-45/rawdata/20150511_AK07_10045.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/37h39-yx833
title: Lp02-fixed

description: WT Lp02 cells were fixed with GA and re-suspended in  PBS.
description: <strong>Tilt Series Date:</strong> 2017-11-20</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Shuman Lab</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-20-35/rawdata/</p>Legionella_fixed_201711200041.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-20-35/rawdata/Legionella_fixed_201711200041.mrc"
        > <i class="download icon"></i> Download </a></p>

        Legionella_fixed_201711200041_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-20-35/3dimage_90367/Legionella_fixed_201711200041_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hq4m9x
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Cyphotilapia frontosa, Cyprichromis leptosoma, Cyprichromis microlepidotus, Cyrtocara moorii, Dagetichthys commersonnii, Synaptura commersonnii, Dagetichthys lakdoensis, Dalophis boulengeri, Dalophis cephalopeltis, Danakilia franchettii, Datnia elongata, Mesopristes elongatus, Denticeps clupeoides, Dermatopsoides kasougae, Dicentrarchus punctatus, Dicologlossa cuneata, Dimidiochromis compressiceps, Dimidiochromis dimidiatus, Dimidiochromis kiwinge, Dimidiochromis strigatus, Dinotopterus cunningtoni, Diplotaxodon aeneus, Diplotaxodon altus, Diplotaxodon argenteus, Diplotaxodon ecclesi, Diplotaxodon greenwoodi, Diplotaxodon limnothrissa, Diplotaxodon longimaxilla, Diplotaxodon macrops, Distichodus affinis, Distichodus altus, Distichodus antonii, Distichodus atroventralis, Distichodus brevipinnis, Distichodus decemmaculatus, Distichodus engycephalus, Distichodus fasciolatus, Distichodus hypostomatus, Distichodus kolleri, Distichodus langi, Distichodus lusosso, Distichodus maculatus, Distichodus mbiniensis, Distichodus microps, Distichodus mossambicus, Distichodus nefasch, Distichodus noboli, Distichodus notospilus, Distichodus petersii, Distichodus rostratus, Distichodus rufigiensis, Distichodus schenga, Distichodus sexfasciatus, Divandu albimarginatus, Docimodus evelynae, Docimodus johnstoni, Dolichallabes microphthalmus, Dolichamphilius brieni, Dolichamphilius longiceps, Dormitator lebretonis, Doumea angolensis, Doumea chappuisi, Doumea gracila, Doumea sanaga, Doumea thysi, Doumea typica, Drombus bontii, Gobius bontii, Dundocharax bidentatus, Ebomegobius goodi, Eclectochromis ornatus, Ectodus descampsii, Eleotris acanthopoma, Eleotris daganensis, Eleotris fusca, Eleotris mauritiana, Eleotris pellegrini, Eleotris senegalensis, Eleotris vittata, Eleotris vomerodentata, Ellochelon vaigiensis, Elops lacerta, Elops senegalensis, Engraulicypris bredoi, Engraulicypris brevianalis, Engraulicypris gariepinus, Engraulicypris moeruensis, Engraulicypris ngalala, Engraulicypris sardella, Engraulicypris spinifer, Enneacampus ansorgii, Enneacampus kaupi, Enteromius ablabes, Enteromius aboinensis, Enteromius afrohamiltoni, Enteromius afrovernayi, Enteromius aliciae, Enteromius aloyi, Enteromius amanpoae, Enteromius amatolicus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((10.1382 29.0599,1.1382 29.0599,1.1382 20.0599,10.1382 20.0599,10.1382 29.0599))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007982-250827131500795/datasets/export for details.
DOI: 10.22002/87e7h-z8575
title: perfusion_dcrp_fixed

description: This sample was fixed with 2.5% glutaraldehyde for 1 hour. The cells look sick, the outer membrane looks bad. Fixing for 1 hour turns out to be bad,need to fix for lesser time.
description: <strong>Tilt Series Date:</strong> 2015-08-18</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The sample was grown in perfusion chamber and fixed for 1hour with 2.5% glutaraldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1ul gold beads.</p>
<strong>Sample Preparation:</strong> The ice was too thick in the squares for which LM images were available, so could not correlate. Took tomograms of all bugs in a square.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-49/3dimage_60535/</p>perfusion_dcrp_atlas10050_part121_1.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-49/3dimage_60535/perfusion_dcrp_atlas10050_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>

        perfusion_dcrp_atlas10050.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-49/rawdata/perfusion_dcrp_atlas10050.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bddqz-q7t13
title: Halobacterium salinarum

description: Raw data files of Halobacterium salinarum
description: <strong>Tilt Series Date:</strong> 2016-12-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Halobacterium salinarum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard H. salinarum medium</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-12-16-15/3dimage_80653/</p>Hs_201612160014_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-12-16-15/3dimage_80653/Hs_201612160014_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Hs_201612160014.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-12-16-15/rawdata/Hs_201612160014.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7xbpe-prm07
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-04-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-09-4/rawdata/</p>20150409_AK005_10004.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-09-4/rawdata/20150409_AK005_10004.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150409_AK005_10004_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-09-4/3dimage_53765/20150409_AK005_10004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8y4jv-sw930
title: V. cholerae RT4236 (refreshed)

description: Grid box AK15.Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-07-13</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 N1 pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Refreshed in fresh media right before freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: C-flat Cu 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-1/rawdata/</p>20150713_AK15_10001.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-1/rawdata/20150713_AK15_10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150713_AK15_10001_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-1/3dimage_59110/20150713_AK15_10001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e4rp9-drt81
title: Thermococcus koakarensis

description: Imaging Thermococcus koakarensis flagella
description: <strong>Tilt Series Date:</strong> 2016-08-25</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard Tk medium with starch and sulfur</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-38/rawdata/</p>Tk_250820160038.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-38/rawdata/Tk_250820160038.mrc"
        > <i class="download icon"></i> Download </a></p>

        Tk_250820160038_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-25-38/3dimage_78641/Tk_250820160038_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6hdvz-0g865
title: Halobacterium salinarum

description: Archaella and plate
description: <strong>Tilt Series Date:</strong> 2017-05-26</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Halobacterium salinarum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard H. salinarum medium</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-2/rawdata/</p>Halobacterium_20170525a0001.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-2/rawdata/Halobacterium_20170525a0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Halobacterium_20170525a0001_part56_7.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-2/3dimage_84749/Halobacterium_20170525a0001_part56_7.rec"
        > <i class="download icon"></i> Download </a></p>