DOI: 10.6084/m9.figshare.9862139
title: Data files describing the systemic screening of 96 Schistosoma mansoni cell surface and secreted antigens produced in a mammalian expression system

description: In this study, the authors have established an infrastructure for testing a large number of vaccine candidates in mice and used it to screen 96 cell-surface and secreted recombinant proteins from <i>Schistosoma mansoni</i>. This approach, using standardised immunisation and percutaneous infection protocols, allowed comparison of an extensive set of antigens in a systematic manner.<br>This data record consists of nine datasets. Datasets <b>1. Optimisation of the duration of challenge for percutaneous infection.pzf</b>, <b>2. Optimisation of the rest period after immunisation.pzf</b>, <b>4. Half maximal antibody titres.pzf</b>, <b>5. Correlation Eggs vs Total Adults or Mature Females.pzf</b>, <b>7. Cohort analysis - Individual data for antigens 10 and 58.pzf</b> and <b>8. Repeat analysis on larger groups - Proteins 10 and 58.pzf</b> are in <b>.pzf</b> file format. Dataset <b>3. S. mansoni proteins gels.pptx</b> is in <b>.pptx</b> file format. Dataset <b>6. Eggs per gram of liver counted across cohorts.xlsx </b>is in <b>.xlsx</b> file format. Dataset <b>RDS-SPRN-00358.xlsx </b>is in<b> .xlsx </b>file format and shows the data contained within the .pzf datasets. Spreadsheet "1. Duration of challenge" shows the data in .pzf file 1. Spreadsheet "2. Rest period" shows the data in .pzf file 2. Spreadsheet "4. Antibody titres" shows the data in .pzf file 4. Spreadsheets "5a. Correlation eggs-adults" and "5b. Correlation eggs-females" show the data in .pzf file 5. Spreadsheets "7a. Cohort-protein 10" and "7b. Cohort-protein 58" show the data in .pzf file 7. Spreadsheets "8a. Repeat-protein 10" and "8b. Repeat-protein 58" show the data in .pzf file 8.<br>Dataset 1. Optimisation of the duration of challenge for percutaneous infection.pzf includes data on the optimisation of the conditions of percutaneous challenge in BALB/C female mice by testing different durations of exposure to the parasite.<br>Dataset 2. Optimisation of the rest period after immunisation.pzf includes data on the optimisation of the conditions of percutaneous challenge in BALB/C female mice by testing different rest periods between immunisations and infection challenge.<br>Dataset 3. S. mansoni proteins gels.pptx includes SDS-page data on 96 recombinant <i>S. mansoni </i>antigens that were produced in mammalian cells and purified by Ni<sup>2+</sup>-affinity purification (their integrity was checked by SDS-page before immunisations in BALB/C female mice).<br>Dataset 4. Half maximal antibody titres.pzf includes data on the half-maximal antibody titres. These were derived by immunising each group of mice (5 animals in each group) with an antigen of interest and one control who received PBS. Eight days after the final immunisations, blood samples were collected from each animal and the half-maximal antibody titres against the antigen of interest were determined.<br>Dataset 5. Correlation Eggs vs Total Adults or Mature Females.pzf includes data on the number of eggs and the number of mature female worms counted on euthanized, immunised animals that were challenged by percutaneous infection with <i>S. mansoni </i>cercariae.<br>Dataset 6. Eggs per gram of liver counted across cohorts.xlsx includes data on the number of eggs per gram of liver, that were counted across all cohorts of animals.<br>Dataset 7. Cohort analysis - Individual data for antigens 10 and 58.pzf includes data on individual data points in cohorts corresponding to antigens 10 and 58, for which a statistically significant difference in the number of eggs per gram of liver was observed by ANOVA analysis compared to the other groups from the same cohort.<br>Dataset 8. Repeat analysis on larger groups - Proteins 10 and 58.pzf includes data from repeat experiments when immunisations and challenges were repeated with antigens 10 and 58 on a larger set of animals comprising of twelve experimental and six control animals.<br><b>Study aims and methodology: </b>The study aimed to test the protective efficacy of 96 secreted antigen proteins from <i>Schistosoma mansoni</i>, in immunised mice, against infection with <i>S. mansoni</i>. Recombinant antigens were produced by transient transfection of human embryonic kidney (HEK)-293-E or 6E cells. All animal experiments were performed in accordance with UK Home Office regulations under project licenses P77E8A062 and PD3DA8D1F. For each purified antigen, a group of 5 female BALB/C mice was immunised intraperitoneally. In each group, a sixth animal was used as a cage control. After a 4-week rest period, animals were challenged with <i>S. mansoni </i>cercariae. Forty-two days after the challenge, mice were euthanized and <i>S. mansoni </i>worms were collected from individual mice. Liver tissues were weighed and the number of eggs per gram of liver was calculated. Following immunisation, blood samples were collected from each animal and antibody titres were determined using enzyme-linked immunosorbent assay (ELISA). For more details on the methodology and statistical analysis, see the published article. <br><b>Software needed to access datasets:</b> Datasets in the. pzf file format require the GraphPad Prism software to be accessed. <br>