cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/8c4zbxfvwk.1
title: GERDA datasets

description: pre-ART (antiretroviral therapy), P01_46_T1 and P01_46_VOA_d14 dataset for HIV-1 reservoir screen from Swiss HIV Study Cohort using GERDA (Gag and Envelope reactivation co-detection assay). Resubmitted to Cell Reports Methods (Jan-2023)

GERDA is a new detection method to decipher the HIV-1 cellular reservoir in blood (tissue or any other specimen). It integrates HIV-1 Gag and Env co-detection along with cellular surface markers to reveal 1) what cells still contain HIV-1 translation competent virus and 2) which marker the respective infected cells express. The phenotypic marker repertoire of the cells allow to make predictions on potential homing and to assess the HIV-1 (tissue) reservoir.
All FACS data were acquired on a LSRFortessa BD FACS machine (markers: CCR7, CD45RA, CD28, CD4, CD25, PD1, IntegrinB7, CLA, HIV-1 Env, HIV-1 Gag)
Raw FACS data (pre-gated CD4CD3+ T-cells) were arcsin transformed and dimensionally reduced using optsne. Data was further clustered using DBSCAN and either individual clusters were further analyzed for individual marker expression or expression profiles of all relevant clusters were analyzed by heatmaps.

data normalization code (by Julian Spagnuolo)
FACS normalized data as CSV (XXX_arcsin.csv)
OMIQ conText file (_OMIQ-context_XXX)
arcsin normalized FACS data after optsne dimension reduction with OMIQ.ai as CSV file (XXXarcsin.csv.csv)
R pipeline with codes (XXX_commented.R)
DOI: 10.17632/8c4zbxfvwk.3
title: GERDA datasets including NGS and SGA data

description: Datasets linked to publication "Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods", Otte et al 2023 published in Cell Reports Methods
pre-ART (antiretroviral therapy) cryo-conserved and and whole blood specimen were sampled for HIV-1 virus reservoir determination in HIV-1 positive individuals from the Swiss HIV Study Cohort. Patients were monitored for proviral (DNA), poly-A transcripts (RNA), late protein translation  (Gag and Envelope reactivation co-detection assay, GERDA) and intact viruses (golden standard: viral outgrowth assay, VOA).
In this dataset we deposited the pipeline for the multidimensional data analysis of our newly established GERDA method, using DBScan and tSNE. For further comprehension NGS and Sanger sequencing data were attached as processed and raw data (GenBank).

Resubmitted to Cell Reports Methods (Jan-2023), accepted in principal (Mar-2023)

GERDA is a new detection method to decipher the HIV-1 cellular reservoir in blood (tissue or any other specimen). It integrates HIV-1 Gag and Env co-detection along with cellular surface markers to reveal 1) what cells still contain HIV-1 translation competent virus and 2) which marker the respective infected cells express. The phenotypic marker repertoire of the cells allow to make predictions on potential homing and to assess the HIV-1 (tissue) reservoir.
All FACS data were acquired on a LSRFortessa BD FACS machine (markers: CCR7, CD45RA, CD28, CD4, CD25, PD1, IntegrinB7, CLA, HIV-1 Env, HIV-1 Gag)
Raw FACS data (pre-gated CD4CD3+ T-cells) were arcsin transformed and dimensionally reduced using optsne. Data was further clustered using DBSCAN and either individual clusters were further analyzed for individual marker expression or expression profiles of all relevant clusters were analyzed by heatmaps.
Sequences before/after therapy initiation and during viral outgrowth cultures were monitored for individuals P01-46 and P04-56 by Next-generation sequencing (NGS of HIV-1 Envelope V3 loop only)  and by Sanger (single genome amplification, SGA)

data normalization code (by Julian Spagnuolo)
FACS normalized data as CSV (XXX_arcsin.csv)
OMIQ conText file (_OMIQ-context_XXX)
arcsin normalized FACS data after optsne dimension reduction with OMIQ.ai as CSV file (XXXarcsin.csv.csv)
R pipeline with codes (XXX_commented.R)
P01_46-NGS and Sanger sequences
P04_56-NGS and Sanger sequences
DOI: 10.17632/gbvh8rjwhc.1
title: Prevalence of Ankle-foot pain and associated factors among medical and physiotherapy students in MM(DU) Mullana, Haryana, India

description: Ankle-foot pain a common musculoskeletal problem that affects people of any age, including health care providers like medical and physiotherapy students. Due to the nature of the careers, they have, require lengthy standing, prolonged walking, long hours of posting, and low shoe comfort, these people are more likely to get ankle-foot pain. Ankle-foot pain may have a major impact on a person's quality of life and may make it difficult for them to carry out daily tasks, including studies.
DOI: 10.17632/fkbkf3xgck.2
title: Optimization of bioethanol production from banana peels

description: Energy plays a vital role in the development of any nation. However, that development increases pollution levels. Recycling and utilization have become major concerns of developing countries. The goal of this research is to utilize banana peels for the production of bioethanol by using the yeast Saccharomyces cerevisiae. The effects of hydrolysis factors were investigated, and the optimized combination of variables with response surface designs was found. The results show that 45.088% of ethanol was obtained at 1.50% v/v acid concentration, 91.02 °C temperature and 21.66 min retention time. Analysis of variance (ANOVA) correlation coefficients Pred R2 = 0.8650 and Adj R2 = 0.9782 indicates an excellent evaluation of the experimental data by a second-order polynomial regression model.
DOI: 10.17632/fkbkf3xgck.1
title: EtOH from Banana Peels

description: BSc thesis work data
DOI: 10.17632/fkbkf3xgck.3
title: Optimization of bioethanol production from banana peels

description: Energy plays a vital role in the development of any nation. However, that development increases pollution levels. Recycling and utilization have become major concerns of developing countries. The goal of this research is to utilize banana peels for the production of bioethanol by using the yeast Saccharomyces cerevisiae. The effects of hydrolysis factors were investigated, and the optimized combination of variables with response surface designs was found. The results show that 45.088% of ethanol was obtained at 1.50% v/v acid concentration, 91.02 °C temperature and 21.66 min retention time. Analysis of variance (ANOVA) correlation coefficients Pred R2 = 0.8650 and Adj R2 = 0.9782 indicates an excellent evaluation of the experimental data by a second-order polynomial regression model.
DOI: 10.17632/xf8dz7962j.1
title: Examining the Relationship of National Values, Adaptive Culture, Transformational Leadership, and Strategic Human Resource Management to Organizational Resilience: A Quantitative Studey in the Philippine Government

description: This study examines the effects of an organization’s human resources, leadership, culture, and adaptation of national values to organizational resilience in the context of the Philippine public sector. Through a questionnaire administered to 186 respondents, results of the path analysis conducted using Jamovi revealed that strategic human resource management showed significant mediation paths for the relationship between national values and organizational resilience [NV→HR (B = 0.33, SE = 0.08, p < 0.001) and HR→OR (B = 0.17, SE = 0.06, p = 0.016)] and between adaptive culture and organizational resilience [AC→HR (B = 0.48, SE = 0.13, p < 0.001) and HR→OR (B = 0.17, SE = 0.06, p = 0.016)]; and that the path analysis model also converged with its predictions matching the actual data well (SRMR = 0.000 and CFI = 0.000).
DOI: 10.17632/465b2ty66p.1
title: Factors influencing physiotherapists to make a choice of specialization for their post graduation

description: People now seek out improved evidence-based treatment due to advancements in health studies and increased public awareness. This was a cross-sectional online survey study conducted at MMIPR, (MMDU). The questionnaire was distributed to physiotherapy students via Whatsapp and E-mail. Out of 380, 241 responded and making a response rate of 63.42%. Of students who want higher education in physiotherapy 68.8% (166) and 23.6% (57) are not sure. The major factor influencing was their interest in the subject (89.2%), the possibility of high potential salary (76.6%), clinical perspective (80.2%), clinical postings (77.5%), employment perspective (70.8%), and serve the community and help others (60.9%). The major factor influencing physiotherapists was their interest in the subject, clinical perspective, experience during clinical posting, employment perspective, the possibility of high potential salary, serve the community/ helping others.
DOI: 10.17632/99rgnnvhfr.1
title: LSB mendelian

description: Role of Leisure Sedentary Behavior in the Increased Prevalence of Type 2 Diabetes and Impaired Glycemic Homeostasis: A Mendelian Randomized Study
DOI: 10.17632/mjn83psf9c.1
title: ExMD: SU(2) matrix decomposition

description: In quantum information science, Berry’s geometric phase, corresponding to the evolution of the quantum system, has important information. Notably, the geometric phase is essential in developing fault-tolerant quantum computing. However, a general description of the geometric phase has not yet been reported, especially for non-commuting and non-anticommuting properties (we call these non-diabolical properties). We explain the cyclic evolution of arbitrary quantum states using multiple rotation operators with non-diabolical properties, and compare it with the quantum evolution by commuting and anticommuting properties. In addition, we analyze the conditions of such evolution in terms of geometric and dynamic phases using Uhlmann’s fidelity and visualization on the Bloch sphere, drawn in the ExMD Mathematica package.
DOI: 10.17632/c5fkv7k2nr.1
title: Hydraulic stimulation data of the St1 deep wells OTN-2 and OTN-3, Finland

description: Hydraulic stimulation data of the OTN-2 and OTN-3 wells
DOI: 10.17632/3k5y2jcpk5.1
title: Data on microstructural and optoelectronic properties of electrodeposited silver mesh transparent conducting electrodes

description: This dataset contains microstructural and optoelectronic properties of electrodeposited silver mesh transparent conducting electrodes
DOI: 10.17632/jpvk5rjksk.1
title: Study of MK-associated phage

description: Data sets related to "Combined actions of bacteriophage-encoded genes in Wolbachia-induced male lethality" (iScinece).
DOI: 10.5281/zenodo.7393879
title: The spectral slope of chromophoric dissolved organic matter (S275-295) as physical and biogeochemical tracers in the largest marginal sea in western Pacific

description: The present study is focused on the CDOM vertical profiles in the northern South China Sea. The results suggest humic-like FDOM is controlling the variation of the spectral slope of CDOM (<em>S</em><sub>275-295</sub>). In addition, our results suggest <em>S</em><sub>275-295</sub> could be used as tracers of water mass, CDOM diversity and radiocarbon age of dissolved organic carbon in oligotrophic ocean.
DOI: 10.6084/m9.figshare.22693042
title: DFT data for figures

description: Raw data of VASP configuration ifles, selected VASP output files, and scripts for generating the figure plots related to this research.
DOI: 10.5281/zenodo.7866043
title: CDOM spectral slope (S275-295) as tracers of water masses, CDOM heterogeneity, and 14C-DOC in an oligotrophic marginal sea

description: The present study is focused on the CDOM vertical profiles in the northern South China Sea. The results suggest humic-like FDOM is controlling the variation of the spectral slope of CDOM (<em>S</em><sub>275-295</sub>). In addition, our results suggest <em>S</em><sub>275-295</sub> could be used as tracers of water mass, CDOM diversity and radiocarbon age of dissolved organic carbon in oligotrophic ocean.
DOI: 10.5281/zenodo.7393878
title: CDOM spectral slope (S275-295) as tracers of water masses, CDOM heterogeneity, and 14C-DOC in an oligotrophic marginal sea

description: The present study is focused on the CDOM vertical profiles in the northern South China Sea. The results suggest humic-like FDOM is controlling the variation of the spectral slope of CDOM (<em>S</em><sub>275-295</sub>). In addition, our results suggest <em>S</em><sub>275-295</sub> could be used as tracers of water mass, CDOM diversity and radiocarbon age of dissolved organic carbon in oligotrophic ocean.
DOI: 10.17632/d53y7b28kf.1
title: supervisees' perceptions of how often and how supervisors address countertansference

description: Participants were 78 supervisees who reported demographic information about themselves.  They also were identified a specific instance that stood out to them (for any reason) in which they had addressed countertransference (CT) in supervision.  They then described characteristics of their supervisor and the client. 
They completed The Supervisory Working Alliance Inventory–Trainee Short Form and a six-item adaptation of the Clinical Outcomes scale of Tsong and Goodyear’s (2014)  Supervision Outcome Scale.  They also were asked to report (a) how often their supervisors addressed CT, (b) who identified this instance of CT (their supervisor, they the supervisee, or both together), (c) the extent to which the supervisor adopted a therapist role, (d) the extent to which the supervisor was perceived to pathologize them, (e ) the effect of this experience on them, and (f) the extent to which their CT reaction matched 14 types of possible CT reactions
DOI: 10.17632/dk2zvnj2c3.1
title: Codes for: Free-ridership in subsidies for company and private electric vehicles.

description: Includes a Stata do-file to estimate the free rider share and the cost-effectiveness of Germany's subsidy program for electric vehicles using high-dimensional vehicle registration panel data from 2014 to 2021. Additionally, an Excel file is provided, which contains the processed data at the aggregate level, as well as the descriptive statistics reported in the paper.
DOI: 10.17632/pfs8yt8krc.1
title: research data

description: we have demonstrated that ASR significantly affects contest and courtship behaviour in the dung beetle Onthophagus taurus. ASR is an accurate predictor of the intensity of contest and courtship competition, with the male-biased treatment exhibiting higher levels of these behaviours. In addition, we found no significant difference in the weight of brood balls among the different ASR treatments. The mean number of broods per male was lower in the male-biased treatment compared to the female-biased and unbiased treatments, while the mean number of brood balls per female did not differ significantly among ASR treatments. These findings suggest that ASR may impair the breeding success of dung beetles by influencing their fecundity, but not parental care.
DOI: 10.17632/rdfwst7xxb.1
title: Species list, life zones, and vegetation types for Caatinga

description: A list of floristic studies in Caatinga was compiled to test the hypothesis that edaphic differentiation (crystalline vs sedimentary terrains) affects the distribution of Holdridge life zones, vegetation types, and beta diversity. The first spreadsheet has the species list by sampling area and terrain, the second has the life zones for 1,208 municipalities, the third has the vegetation type, and the fourth has the presence/absence of species to create a table of area pairs in the GDM format.
DOI: 10.17632/gr6j9h7rbd.1
title: Impact of Prolonged Wrist Extension as the Prevalent Cause of Wrist Pain among Computer Desktop Workers- A Survey Study

description: Objective: - The study focuses on determining the impact of prolonged wrist extension on wrist pain among computer desktop workers. It is observed that prolonged wrist extension may lead a person to develop wrist pain. Long-lasting wrist pain affects the daily working performance of the users by restricting their activities and ability to cope with the demanding workload.
Material and Method: -  This study was a cross-sectional study. 302 computer desktop workers aged 18 to 60 years participated in an online survey.
Result: - The study shows that about 85.09% of participants had wrist pain during typing. 12.9% of participants, felt wrist pain in their left hand, 30.4% of participants had pain in both hands and 56.6% of participants had wrist pain in their right hand.
Conclusion: - The study concluded that wrist pain was prevalent among computer desktop workers. The pain was felt, and symptoms were connected to a particular person and job-related characteristics. With no threshold effects, typing time indicated wrist pain at low exposure levels.
DOI: 10.17632/w3jkrw2ct8.1
title: 3D printed wind-tunnel for calibrating bidirectional velocity probes in fire engineering

description: This is 3D printed wind tunnel with Arduino-based unit.

This source describes Open Hardware and is licensed under the CERN-OHL-S v2

Experience with Arduino platform is required and supervision from a qualified electric engineering professional.

The project is prototype under development and authors are not responsible for any damages or injuries caused by inappropriate construction or operation. This source is distributed WITHOUT ANY EXPRESS OR IMPLIED WARRANTY, INCLUDING OF MERCHANTABILITY, SATISFACTORY QUALITY AND FITNESS FOR A PARTICULAR PURPOSE.

References
B.J. McCaffrey, G. Heskestad,A robust bidirectional low-velocity probe for flame and fire application, Combustion and Flame, Volume 26, 1976, Pages 125-127, ISSN 0010-2180, https://doi.org/10.1016/0010-2180(76)90062-6.
Mauro, Stefano & Lanzafame, Rosario & Messina, Michele & Brusca, S. & Famoso, Fabio & Galvagno, Antonio. (2017). Small-Scale Open-Circuit Wind Tunnel: Design Criteria, Construction and Calibration. International Journal of Applied Engineering Research. 12. 13649-13662.
The JeeLabs Shop. (n.d.). Retrieved February 11, 2021, from https://www.digitalsmarties.net/products/wind-sensor-rev-p
Wind sensor REV. p - low Cost Anemometer. (n.d.). Retrieved February 12, 2021, from https://moderndevice.com/product/wind-sensor-rev-p/
How can i tell if a case fan is sucking or blowing air? (2019, December 30). Retrieved February 13, 2021, from https://www.computerhope.com/issues/ch001151.htm
Prohasky, Daniel & Watkins, Simon. (2014). Low cost hot-element anemometry versus the TFI Cobra.
Thingiverse.com. (n.d.). The ultimate box maker BY HEARTMAN. Retrieved February 12, 2021, from https://www.thingiverse.com/thing:1264391
T H Yong and S S Dol 2015 IOP Conf. Ser.: Mater. Sci. Eng. 78 012039
DOI: 10.17632/w3jkrw2ct8.2
title: Setup of a 3D printed wind tunnel: application for calibrating bi-directional velocity probes used in Fire Engineering Applications

description: The research presented here focuses on the development of a 3D printed wind tunnel and the relevant equipment to be used for calibrating bi-directional velocity probes (BDVP). BDVP are equipment to be used for measuring velocity flow by determining the pressure difference of hot gases generated during fires. The manufactured probes require calibration to determine the calibration factor to achieve precise measurement. The calibration is usually performed in wind tunnels which can be difficult to access due to costs, complexity and the various pieces of equipment required. The aim of the current study is to develop and assemble an inexpensive and easy-to-build bench-scale wind tunnel, with a data-logging system and fan control functionalities for fast and effective calibration of BDVP. A 3D printer with a PET-G filament is used, able to produce parts for the wind tunnel system which are durable and easy to handle and assemble. The system additionally includes an Arduino-based measuring unit with a hot-wire anemometer and temperature correction: Rev. P. This takes precise measurements; continuously logging data on a computer through a USB interface and capable of saving data on an SD card. This design provides users with parameters of velocity flow up to 4 m/s with standard deviation of 1.2 % and turbulence intensity of 1 %. The main advantages of this wind tunnel are its simplicity to build and portability.

The dataset contains design files for 3d printing of the wind tunnel, BOM and wiring of electronic components. 

The project is prototype under development and authors are not responsible for any damages or injuries caused by inappropriate construction or operation. This source is distributed WITHOUT ANY EXPRESS OR IMPLIED WARRANTY, INCLUDING OF MERCHANTABILITY, SATISFACTORY QUALITY AND FITNESS FOR A PARTICULAR PURPOSE.
DOI: 10.17632/4cvwmvdgnj.1
title: A novel metabarcoded deep amplicon sequencing tool for the detection of diminazene drug resistance mutations in Trypanosoma evansi

description: Diminazene resistance of Trypanosoma is an increasing problem whose underlying genetics are not fully understood. The development of diminazene resistance in Trypanosoma brucei is associated with loss of drug uptake by the P2 aminopurine due to alteration in the corresponding adenosine transporter-1 (AT-1) domain. Specific mutant-type alleles (71V, 178T, 181E, 239G, 286S and 380P) linked to resistance were more prevalent in T. brucei subspecies gambiense in humans. In the present study, we have analysed the adenosine transporter-1 (AT-1) domain of the P2 aminopurine from Trypanosoma evansi to explore a possible genetic link between the presence of mutations in this gene and diminazene treatment in ruminants. The genetic basis of diminazene resistance was investigated with a new high-throughput metabarcoded deep amplicon sequencing tool in T. evansi field isolates. We examined Trypanosoma isolates collected from ruminants (Camel, Buffalo, Cattle, Goat, Sheep) in seven known endemic regions of the Punjab province of Pakistan, where animals are treated in a sporadic manner, often with generic diminazene drugs of unknown quality. Blood samples were collected for DNA preparation, adapter/barcoded PCR amplification and Illumina Mi-Seq based sequencing. Our results indicate that 7  T. evansi field isolates had resistance mutations at codon 178E/S, 239Y/A/E, 286S/H/I/D/T in the adenosine transporter-1 (AT-1) domain. In contrast, 26 T. evansi field isolates had susceptible mutations at codon A178, G181, D239 and N286 in the adenosine transporter-1 (AT-1) domain. The overall outcomes will be useful in understanding the emergence of diminazene resistance. This information will help design strategies to optimize the use and lifespan of the most important drug to control trypanosomiasis.
DOI: 10.17632/dym93kv36x.1
title: PFAS in Water, Soil, and Sediment of the Fountain Creek Watershed, Colorado Springs, CO, USA

description: Ten perfluorinated alkyl substances (PFAS), including linear and branched isomers, were surveyed in samples collected from the Fountain Creek Watershed. Water, soil, and sediment samples were collected monthly from eight locations in the Fountain Creek Watershed for a full year (May 2018 through May 2019). Environmental samples were prepared via solid phase extraction (SPE) prior to high-performance liquid chromatography tandem mass spectrometry (LC/MS/MS) analysis on a Shimadzu LCMS-8030 system with coupled LC fitted with a Waters XBridge C18 column (50 mm x 4.6 mm x 3 micron particle). These data are useful for investigators interested in the long-term environmental presence and impact of PFAS released into the environment. We hypothesized that PFAS concentrations in water, soil, and sediment samples would be statistically significantly higher (P < 0.05) in environmental samples south of Peterson Air Force Base (PAFB). These data are of interest to investigators and entities seeking to understand the longer term impact of PFAS release into surface and groundwater resources.