cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19920589
title: Taxonomic and functional turnover of Amazonian stream fish assemblages is determined by deforestation history and environmental variables at multiple scales

description: Abstract High rates of deforestation, either in the past or the present, affect many of the ecological processes in streams. Integrating deforestation history and the current landscape structure enhances the evaluation of ecological effects of land-use change. This is especially true when contemporary landscape conditions are similar but the temporal path to those conditions differs. One approach that has shown promise for evaluating biodiversity responses over time and space is the β-diversity partitioning, which combines taxonomic and functional trait-based approaches. We tested hypotheses related to stream fish assemblages’ turnover in watersheds with different environmental conditions and deforestation histories. We sampled fish from 75 watersheds in the Machado River basin, Brazil, and environmental factors were quantified at multiple scales. Taxonomic turnover was higher than expected by chance, whereas functional turnover was lower than expected by the observed taxonomic turnover, indicating that deterministic processes are structuring these assemblages. The turnover, and the environmental factors differed among watersheds with different deforestation histories. Besides being scale-dependent, turnover patterns are also likely dependent on land use dynamics and involve time-lags.
DOI: 10.6084/m9.figshare.19920589.v1
title: Taxonomic and functional turnover of Amazonian stream fish assemblages is determined by deforestation history and environmental variables at multiple scales

description: Abstract High rates of deforestation, either in the past or the present, affect many of the ecological processes in streams. Integrating deforestation history and the current landscape structure enhances the evaluation of ecological effects of land-use change. This is especially true when contemporary landscape conditions are similar but the temporal path to those conditions differs. One approach that has shown promise for evaluating biodiversity responses over time and space is the β-diversity partitioning, which combines taxonomic and functional trait-based approaches. We tested hypotheses related to stream fish assemblages’ turnover in watersheds with different environmental conditions and deforestation histories. We sampled fish from 75 watersheds in the Machado River basin, Brazil, and environmental factors were quantified at multiple scales. Taxonomic turnover was higher than expected by chance, whereas functional turnover was lower than expected by the observed taxonomic turnover, indicating that deterministic processes are structuring these assemblages. The turnover, and the environmental factors differed among watersheds with different deforestation histories. Besides being scale-dependent, turnover patterns are also likely dependent on land use dynamics and involve time-lags.
DOI: 10.6084/m9.figshare.19921883
title: "THE SUN OF FREEDOM"? THE EDUCATION OF ENSLAVED, FREEDMEN AND INGENUOUS (1871-1875)

description: ABSTRACT: In this article, we seek to give visibility to the speeches made in the House of Representatives and in the Senate about the approval of the Free Womb Law. After all, how did the enslaved population face the new situation and what measures were taken to abolish what some considered as the "blackest slavery: ignorance"? To explore this question, we analyze the debates that took place during the drafting, processing and approval of the Law of September 28, 1871, the so-called "Free Womb Law", based on documents from the House of Representatives and the Senate, produced during the administration of João Alfredo Corrêa de Oliveira at the Ministry of the Empire's Businesses (1871-1975). With this investment, we analyze some aspects related to the access to formal or informal schooling of blacks, enslaved, freedmen and ingénues. In order to reflect on the effects of this project, we consulted printed materials circulating at that time, such as Gazeta da Tarde (Evening Gazette), A Instrucção Pública (The Public Instruction), Illustração Brasileira (Brazilian Illustration) and Jornal do Commercio (Trade Newspaper), in which we found important information about the debates and positions of this reform in the legislative apparatus and in public opinion. The mentioned printed materials also functioned as important sources to understand aspects and challenges of the schooling of blacks, slaves and ingenuous in the focused period.
DOI: 10.6084/m9.figshare.19921883.v1
title: "THE SUN OF FREEDOM"? THE EDUCATION OF ENSLAVED, FREEDMEN AND INGENUOUS (1871-1875)

description: ABSTRACT: In this article, we seek to give visibility to the speeches made in the House of Representatives and in the Senate about the approval of the Free Womb Law. After all, how did the enslaved population face the new situation and what measures were taken to abolish what some considered as the "blackest slavery: ignorance"? To explore this question, we analyze the debates that took place during the drafting, processing and approval of the Law of September 28, 1871, the so-called "Free Womb Law", based on documents from the House of Representatives and the Senate, produced during the administration of João Alfredo Corrêa de Oliveira at the Ministry of the Empire's Businesses (1871-1975). With this investment, we analyze some aspects related to the access to formal or informal schooling of blacks, enslaved, freedmen and ingénues. In order to reflect on the effects of this project, we consulted printed materials circulating at that time, such as Gazeta da Tarde (Evening Gazette), A Instrucção Pública (The Public Instruction), Illustração Brasileira (Brazilian Illustration) and Jornal do Commercio (Trade Newspaper), in which we found important information about the debates and positions of this reform in the legislative apparatus and in public opinion. The mentioned printed materials also functioned as important sources to understand aspects and challenges of the schooling of blacks, slaves and ingenuous in the focused period.
DOI: 10.6084/m9.figshare.19905672
title: Dynamic changes in and time sequence of ultraviolet B-induced apoptosis in rat corneal epithelial cells

description: ABSTRACT Purpose: To systematically examine the dynamic changes and time sequence in corneal epithelial cell apoptosis after excessive ultraviolet B irradiation. Methods: Ultraviolet B (144 mJ/cm2) was used to irradiate rat corneal epithelial cells for 2 h. Cell morphology was observed on differential interference contrast microscopy, and the numbers of the different kinds of apoptotic cells were counted using the ImageJ software. Cell viability was measured with the 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2-H-tetrazolium bromide method. Cell apoptotic rate and loss of mitochondrial membrane potential were detected using flow cytometric analyses. The expression levels of 3 apoptotic genes were measured with real-time quantitative polymerase chain reaction at different time points within 0-24 h after irradiation. Results: After 144-mJ/cm2 ultraviolet B irradiation for 2 h, the expression levels of caspase-8 and Bax were highest at 0 h; furthermore, the mitochondrial membrane potential decreased at 0 h and remained constant for 6 h in a subsequent culture. At 6 h, caspase-3 was activated. The decrease in cell viability and increase in apoptotic rate peaked at 6 h. The caspase-3 expression level decreased within 12-24 h, which led to a decline in apoptotic rate and change in apoptotic stage. Conclusions: The corneal epithelial cells exhibited rapid apoptosis after ultraviolet B irradiation, which was associated with both extrinsic and intrinsic pathways.
DOI: 10.6084/m9.figshare.19905672.v1
title: Dynamic changes in and time sequence of ultraviolet B-induced apoptosis in rat corneal epithelial cells

description: ABSTRACT Purpose: To systematically examine the dynamic changes and time sequence in corneal epithelial cell apoptosis after excessive ultraviolet B irradiation. Methods: Ultraviolet B (144 mJ/cm2) was used to irradiate rat corneal epithelial cells for 2 h. Cell morphology was observed on differential interference contrast microscopy, and the numbers of the different kinds of apoptotic cells were counted using the ImageJ software. Cell viability was measured with the 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2-H-tetrazolium bromide method. Cell apoptotic rate and loss of mitochondrial membrane potential were detected using flow cytometric analyses. The expression levels of 3 apoptotic genes were measured with real-time quantitative polymerase chain reaction at different time points within 0-24 h after irradiation. Results: After 144-mJ/cm2 ultraviolet B irradiation for 2 h, the expression levels of caspase-8 and Bax were highest at 0 h; furthermore, the mitochondrial membrane potential decreased at 0 h and remained constant for 6 h in a subsequent culture. At 6 h, caspase-3 was activated. The decrease in cell viability and increase in apoptotic rate peaked at 6 h. The caspase-3 expression level decreased within 12-24 h, which led to a decline in apoptotic rate and change in apoptotic stage. Conclusions: The corneal epithelial cells exhibited rapid apoptosis after ultraviolet B irradiation, which was associated with both extrinsic and intrinsic pathways.
DOI: 10.34740/kaggle/dsv/3718179
title: AutoXGB
DOI: 10.6084/m9.figshare.19921922
title: Repeatability and divergence among genotypes of Desmanthus sp. in a semiarid region

description: Abstract: The objective of this work was to evaluate the divergence among genotypes of Desmanthus sp. regarding morphological, productive, and qualitative characteristics, as well as to estimate the number of observations necessary to predict the real value of these characteristics. The experiment was conducted in the semiarid of the state of Pernambuco, Brazil, using 26 genotypes of Desmanthus sp. from different locations in the region. Seven evaluations were carried out from July 2013 to July 2014. Tocher’s grouping method and standardized average Euclidean distance were used. The coefficients of repeatability (r) and determination (R2) were estimated using two models of the analysis of variance, principal components based on the correlation or covariance matrix, and structural analysis based on the correlation matrix. The variables with the greatest weights were stem diameter, leaf area index, and forage yield, with frequencies of 22.05, 17.57, and 14.58%, respectively. Morphological, productive, and qualitative variability was observed among the genotypes of Desmanthus sp. All characteristics presented r and R2 of high magnitude in the methods of analysis. Up to four evaluation cycles are required to predict (R2=95%) the real value of stem diameter, peduncle length, plant height, leaf length and width, number of leaves, and pod length.
DOI: 10.6084/m9.figshare.19921922.v1
title: Repeatability and divergence among genotypes of Desmanthus sp. in a semiarid region

description: Abstract: The objective of this work was to evaluate the divergence among genotypes of Desmanthus sp. regarding morphological, productive, and qualitative characteristics, as well as to estimate the number of observations necessary to predict the real value of these characteristics. The experiment was conducted in the semiarid of the state of Pernambuco, Brazil, using 26 genotypes of Desmanthus sp. from different locations in the region. Seven evaluations were carried out from July 2013 to July 2014. Tocher’s grouping method and standardized average Euclidean distance were used. The coefficients of repeatability (r) and determination (R2) were estimated using two models of the analysis of variance, principal components based on the correlation or covariance matrix, and structural analysis based on the correlation matrix. The variables with the greatest weights were stem diameter, leaf area index, and forage yield, with frequencies of 22.05, 17.57, and 14.58%, respectively. Morphological, productive, and qualitative variability was observed among the genotypes of Desmanthus sp. All characteristics presented r and R2 of high magnitude in the methods of analysis. Up to four evaluation cycles are required to predict (R2=95%) the real value of stem diameter, peduncle length, plant height, leaf length and width, number of leaves, and pod length.
DOI: 10.6084/m9.figshare.19921943
title: Integrative mind-body practices: concept proposal for the field of Traditional and Complementary Medicine

description: Abstract The Brazilian National Policy on Integrative and Complementary Practices (PNPIC) was published in 2006. Integrative mind-body practices are part of that policy and they are offered in Brazilian health care services. The concept of Integrative Mind-Body Practices is not consolidated in the literature and its use in government documents is imprecise. The article aimed at proposing a definition for that concept based on the experience of groups of Integrative Mind-Body Practices in Primary Health Care in Florianópolis, Brazil, as well as offering an alternative to the problems of registration and monitoring of the PNPIC. The methodology adopted was Grounded Theory (GT) in its interpretative and constructivist perspective, upon which a formal theory was developed. The data were produced through observations of the activities, conversations with professionals, and interviews with users of groups of Yoga, Lian Gong, Qi Gong, and Circular Dances. The data were coded, validated, and analyzed according to GT steps. The process of devising the concept resulted from the interconnection of the meanings ascribed by participants in contrast to the literature. This analysis enabled us to outline the phenomenon and propose a designation aligned with the PNPIC.
DOI: 10.6084/m9.figshare.19921943.v1
title: Integrative mind-body practices: concept proposal for the field of Traditional and Complementary Medicine

description: Abstract The Brazilian National Policy on Integrative and Complementary Practices (PNPIC) was published in 2006. Integrative mind-body practices are part of that policy and they are offered in Brazilian health care services. The concept of Integrative Mind-Body Practices is not consolidated in the literature and its use in government documents is imprecise. The article aimed at proposing a definition for that concept based on the experience of groups of Integrative Mind-Body Practices in Primary Health Care in Florianópolis, Brazil, as well as offering an alternative to the problems of registration and monitoring of the PNPIC. The methodology adopted was Grounded Theory (GT) in its interpretative and constructivist perspective, upon which a formal theory was developed. The data were produced through observations of the activities, conversations with professionals, and interviews with users of groups of Yoga, Lian Gong, Qi Gong, and Circular Dances. The data were coded, validated, and analyzed according to GT steps. The process of devising the concept resulted from the interconnection of the meanings ascribed by participants in contrast to the literature. This analysis enabled us to outline the phenomenon and propose a designation aligned with the PNPIC.
DOI: 10.6084/m9.figshare.19921976
title: Dihydronaphthalenone chalconoid derivatives as potential cathepsin B inhibitors; design, synthesis, cytotoxicity evaluation and docking analysis

description: Cathepsin B, an abundant expressed cysteine peptidase, plays a key role in cancer cell proliferation, tumor metastasis, apoptosis, angiogenesis, invasion and migration. Therefore, development of cathepsin B inhibitors to treat cancer is of great significance. In this study, dihydronaphthalenone chalconoid derivatives containing different benzyliden moieties were synthesized via an efficient route in microwave condition that resulted in the desired compounds in high yields compared to acid- or base-catalyzed refluxing conditions. Cytotoxicity of the compounds was evaluated against K562, HT-29 and MCF-7 human cancer cell lines by MTT assay. P1, P3 and P9 (containing 4-OCH3, 3-NO2 and 4-CN moieties on phenyl ring, respectively) exhibited good cytotoxic activity with an IC50 range of 7.1-28.9 μM. Molecular docking analysis was carried out to investigate the possible interactions and binding modes of all compounds with cathepsin B. The most promising compounds, P1, P3 and P9 were well accommodated within the active site and had the least estimated free binding energies. It was concluded from both MTT assay and docking studies that some dihydronaphthalenone chalconoid derivatives could be suggested as effective cytotoxic agents and potential cathepsin B inhibitors
DOI: 10.6084/m9.figshare.19921976.v1
title: Dihydronaphthalenone chalconoid derivatives as potential cathepsin B inhibitors; design, synthesis, cytotoxicity evaluation and docking analysis

description: Cathepsin B, an abundant expressed cysteine peptidase, plays a key role in cancer cell proliferation, tumor metastasis, apoptosis, angiogenesis, invasion and migration. Therefore, development of cathepsin B inhibitors to treat cancer is of great significance. In this study, dihydronaphthalenone chalconoid derivatives containing different benzyliden moieties were synthesized via an efficient route in microwave condition that resulted in the desired compounds in high yields compared to acid- or base-catalyzed refluxing conditions. Cytotoxicity of the compounds was evaluated against K562, HT-29 and MCF-7 human cancer cell lines by MTT assay. P1, P3 and P9 (containing 4-OCH3, 3-NO2 and 4-CN moieties on phenyl ring, respectively) exhibited good cytotoxic activity with an IC50 range of 7.1-28.9 μM. Molecular docking analysis was carried out to investigate the possible interactions and binding modes of all compounds with cathepsin B. The most promising compounds, P1, P3 and P9 were well accommodated within the active site and had the least estimated free binding energies. It was concluded from both MTT assay and docking studies that some dihydronaphthalenone chalconoid derivatives could be suggested as effective cytotoxic agents and potential cathepsin B inhibitors
DOI: 10.6084/m9.figshare.19921971
title: Odontogenic gene expression profile of human dental pulp-derived cells under high glucose influence: a microarray analysis

description: Abstract Hyperglycemia, a major characteristic of diabetes, is considered to play a vital role in diabetic complications. High glucose levels have been found to inhibit the mineralization of dental pulp cells. However, gene expression associated with this phenomenon has not yet been reported. This is important for future dental therapeutic application. Objective Our study aimed to investigate the effect of high glucose levels on mineralization of human dental pulp-derived cells (hDPCs) and identify the genes involved. Methodology hDPCs were cultured in mineralizing medium containing 25 or 5.5 mM D-glucose. On days 1 and 14, RNA was extracted and expression microarray performed. Then, differentially expressed genes (DEGs) were selected for further validation using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. Cells were fixed and stained with alizarin red on day 21 to detect the formation of mineralized nodules, which was further quantified by acetic acid extraction. Results Comparisons between high-glucose and low-glucose conditions showed that on day 1, there were 72 significantly up-regulated and 75 down-regulated genes in the high-glucose condition. Moreover, 115 significantly up- and 292 down-regulated genes were identified in the high-glucose condition on day 14. DEGs were enriched in different GO terms and pathways, such as biological and cellular processes, metabolic pathways, cytokine–cytokine receptor interaction and AGE-RAGE signaling pathways. RT-qPCR results confirmed the significant expression of pyruvate dehydrogenase kinase 3 (PDK3), cyclin-dependent kinase 8 (CDK8), activating transcription factor 3 (ATF3), fibulin-7 (Fbln-7), hyaluronan synthase 1 (HAS1), interleukin 4 receptor (IL-4R) and apolipoprotein C1 (ApoC1). Conclusions The high-glucose condition significantly inhibited the mineralization of hDPCs. DEGs were identified, and interestingly, HAS1 and Fbln-7 genes may be involved in the glucose inhibitory effect on hDPC mineralization.
DOI: 10.6084/m9.figshare.19921971.v1
title: Odontogenic gene expression profile of human dental pulp-derived cells under high glucose influence: a microarray analysis

description: Abstract Hyperglycemia, a major characteristic of diabetes, is considered to play a vital role in diabetic complications. High glucose levels have been found to inhibit the mineralization of dental pulp cells. However, gene expression associated with this phenomenon has not yet been reported. This is important for future dental therapeutic application. Objective Our study aimed to investigate the effect of high glucose levels on mineralization of human dental pulp-derived cells (hDPCs) and identify the genes involved. Methodology hDPCs were cultured in mineralizing medium containing 25 or 5.5 mM D-glucose. On days 1 and 14, RNA was extracted and expression microarray performed. Then, differentially expressed genes (DEGs) were selected for further validation using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. Cells were fixed and stained with alizarin red on day 21 to detect the formation of mineralized nodules, which was further quantified by acetic acid extraction. Results Comparisons between high-glucose and low-glucose conditions showed that on day 1, there were 72 significantly up-regulated and 75 down-regulated genes in the high-glucose condition. Moreover, 115 significantly up- and 292 down-regulated genes were identified in the high-glucose condition on day 14. DEGs were enriched in different GO terms and pathways, such as biological and cellular processes, metabolic pathways, cytokine–cytokine receptor interaction and AGE-RAGE signaling pathways. RT-qPCR results confirmed the significant expression of pyruvate dehydrogenase kinase 3 (PDK3), cyclin-dependent kinase 8 (CDK8), activating transcription factor 3 (ATF3), fibulin-7 (Fbln-7), hyaluronan synthase 1 (HAS1), interleukin 4 receptor (IL-4R) and apolipoprotein C1 (ApoC1). Conclusions The high-glucose condition significantly inhibited the mineralization of hDPCs. DEGs were identified, and interestingly, HAS1 and Fbln-7 genes may be involved in the glucose inhibitory effect on hDPC mineralization.
DOI: 10.6084/m9.figshare.19921994
title: Weathering of soils originated from diabase in the Paraná Basin, São Paulo State, Brazil

description: ABSTRACT Weathering of the parent material is the initial process that plays an essential role in soil fertility. The study of this process has become crucial to better understand soil dynamics. Most studies on weathering are based on indexes that calculate the feldspar dissolution rate (for example, CIA, CIW, and PIA) which not always reflect the complexity in the soil profile. Other methodologies have been designed to improve these approaches, such as geochemical balance, difference of elemental contents (DE), and the W index. This work aim to assess the efficiency of these methods to identify variations in recent soil profiles developed from diabase as compared to the CIA, CIW, and PIA indexes. Data on the main oxides were used to calculate the geochemical balance, DE, CIA, CIW, PIA, and W indexes to analyze weathering intensity in two profiles originated from diabase in the São Paulo State, Brazil. The Ki and Kr indexes and Feo /Fed ratio were also factored and we analyzed the mineralogy of soil horizons and the source rock. The W index and DE presented greater weathering variation than the CIA, CIW, and PIA in both profiles. The geochemical balance, mineralogy, and the Ki, Kr and Feo /Fed ratios explained the variations between the horizons. The DE and geochemical balance together generated new information for the analysis of the weathering process than the isolated indexes.