cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5126029.v1
title: Supplementary Material for: No Mutations in the <b><i>PSMC3IP </i></b>Gene Identified in a Swedish Cohort of Women with Primary Ovarian Insufficiency

description: Ovarian dysfunction before the age of 40 years, characterized by hypergonadotropic hypogonadism and presenting with either primary or secondary amenorrhea, is called primary ovarian insufficiency (POI). POI has a significant genetic component, but the specific genetic cause is often unknown. A novel candidate gene for POI, <i>PSMC3IP</i>, has recently been identified. The aim of this study was to investigate a group of patients with POI for possible <i>PSMC3IP</i> mutations. Therefore, DNA samples from 50 patients with POI of primarily Swedish origin were used in the study, 27 with secondary amenorrhea (median age of diagnosis 23 years) and 23 with primary amenorrhea. Control material consisting of DNA samples from 95 women without POI was used for investigation of novel sequence variants. All exons and intron/exon boundaries of the <i>PSMC3IP</i> gene were analyzed by PCR and sequencing. As a result, no pathogenic mutation in the <i>PSMC3IP</i> gene was detected in the cohort. A previously unreported variant, NM_016556.3:c.337+33A&gt;G, was detected in heterozygous form in 1 patient with secondary amenorrhea, likely constituting a normal variant. Two reported single nucleotide polymorphisms were detected in the cohort at the expected frequency. In conclusion, <i>PSMC3IP</i> gene mutations are not common causes of POI in this Swedish cohort.
DOI: 10.6084/m9.figshare.5126002
title: Supplementary Material for: The Integrative Management of Treatment-Resistant Depression: A Comprehensive Review and Perspectives

description: <b><i>Background:</i></b> Major depressive disorder is a prevalent and disabling illness. Notwithstanding numerous advances in the pharmacological treatment of depression, approximately 70% of patients do not remit after first-line antidepressant treatment. <b><i>Methods:</i></b> The MEDLINE/PubMed, EMBASE and ClinicalTrials.gov electronic databases were searched from inception to October 1, 2013, for randomized controlled trials (RCT), relevant open-label trials, meta-analyses and ongoing trials of pharmacological and psychotherapeutic approaches to treatment-resistant depression (TRD). <b><i>Results:</i></b> Switching to a different antidepressant is a useful option following nonresponse to a first-line agent. Although widely used in clinical practice, there is limited evidence to support antidepressant combination for TRD. Notwithstanding evidence for lithium or T<sub>3</sub> augmentation to be successful in TRD, most studies were carried out when participants were treated with tricyclic antidepressants (TCA). Of the available strategies to augment the response to new-generation antidepressants, the use of some atypical antipsychotics is best supported by evidence. Several novel therapeutic options are currently discussed. Evidence suggests that cognitive therapy (CT) is an effective strategy for TRD. <b><i>Conclusions:</i></b> The success of switching to a different antidepressant following a first-line agent is supported by evidence, but there is limited evidence for effective combination strategies. Lithium and T<sub>3</sub> augmentation of TCA have the strongest evidence base for successful treatment of TRD. The use of augmentation of newer-generation antidepressants with atypical antipsychotics is supported by a growing evidence base. Current evidence supports CT as an effective strategy for TRD. There is a need for additional large-scale RCT of TRD. The development of new antidepressants targeting novel pathways opens a promising perspective for the management of TRD.
DOI: 10.6084/m9.figshare.5126008
title: Supplementary Material for: Altered Bacterial Profiles in Saliva from Adults with Caries Lesions: A Case-Cohort Study

description: The aim of this study was to learn whether presence of caries in an adult population was associated with a salivary bacterial profile different from that of individuals without untreated caries. Stimulated saliva samples from 621 participants of the Danish Health Examination Survey were analyzed using the Human Oral Microbe Identification Microarray technology. Samples from 174 individuals with dental caries and 447 from a control cohort were compared using frequency and levels of identified bacterial taxa/clusters as endpoints. Differences at taxon/cluster level were analyzed using Mann-Whitney's test with Benjamini-Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles. A reduced bacterial diversity was observed in samples from subjects with dental caries. Five bacterial taxa (<i>Veillonella parvula</i>, <i>Veillonella atypica</i>, <i>Megasphaera micronuciformis</i>, <i>Fusobacterium periodontium</i> and <i>Achromobacter xylosoxidans</i>) and one bacterial cluster (<i>Leptotrichia sp. clones C3MKM102 and GT018</i>_ot417/462) were less frequently found in the caries group (adjusted p value &lt;0.01) while two bacterial taxa (<i>Solobacterium moorei</i> and <i>Streptococcus salivarius</i>) and three bacterial clusters (<i>Streptococcus parasanguinis I and II and sp. clone BE024</i>_ot057/411/721, <i>Streptococcus parasanguinis I and II and sinensis</i>_ot411/721/767, <i>Streptococcus salivarius and sp. clone FO042</i>_ot067/755) were present at significantly higher levels (adjusted p value &lt;0.01). The principal component analysis displayed a marked difference in the bacterial community profiles between groups. Presence of manifest caries was associated with a reduced diversity and an altered salivary bacterial community profile. Our data support recent theories that ecological stress-induced changes of commensal microbial communities are involved in the shift from oral health to tooth decay.
DOI: 10.6084/m9.figshare.5126011
title: Supplementary Material for: Development and Three-Dimensional Morphology of the Zygomaticotemporal Suture in Primate Skulls

description: Cranial sutures are an essential part of the growing skull, allowing bones to increase in size during growth, with their morphology widely believed to be dictated by the forces and displacements that they experience. The zygomaticotemporal suture in primates is located in the relatively weak zygomatic arch, and externally it appears a very simple connection. However, large forces are almost certainly transmitted across this suture, suggesting that it requires some level of stability while also allowing controlled movements under high loading. Here we examine the 2- and 3-dimensional (3D) morphology of the zygomaticotemporal suture in an ontogenetic series of <i>Macaca fascicularis </i>skulls. High resolution microcomputed tomography data sets were examined, and virtual and physical 3D replicas were created to assess both structure and general stability. The zygomaticotemporal suture is much more complex than its external appearance suggests, with interlocking facets between the adjacent zygomatic and temporal bones. It appears as if some movement is permitted across the suture in younger animals, but as they approach adulthood the complexity of the suture's interlocking bone facets reaches a level where these movements become minimal.
DOI: 10.6084/m9.figshare.5125999
title: Supplementary Material for: Reintroduced Sumatran Orangutans <b><i>(Pongo abelii):</i></b> Using Major Food Tree Species as Indicators of Habitat Suitability

description: Reintroducing orangutans (<i>Pongo </i>spp.) into the wild requires a suitable, secure habitat. To identify acceptable areas for their reintroduction and define priority conservation sites, we analysed the tree species composition in the Bukit Tigapuluh ecosystem in Jambi, Sumatra. We used this information to determine the distribution patterns of those species that represent an essential part of the diet of reintroduced orangutans. Important orangutan food tree species showed significant differences in composition, frequency and abundance among topographic forest types and recovered selectively logged and unlogged forests. Riparian forests and recovered selectively logged areas offered a vegetation composition and forest structure most suitable for the reintroduction of orangutans and showed numerous important tree species that serve as indicator species, i.e. species growing predominantly or exclusively in a specific forest type.
DOI: 10.6084/m9.figshare.5126005
title: Supplementary Material for: Accelerated Reepithelialization by Triterpenes: Proof of Concept in the Healing of Surgical Skin Lesions

description: The acceleration of wound healing is a major surgical concern. A triterpene extract from birch bark (Betulae cortex) experimentally enhances keratinocyte differentiation in vitro and accelerates wound healing ex vivo. We conducted an open, blind-evaluated, controlled, prospective, randomized (1:1) phase II clinical trial in patients requiring split-thickness skin graft transplantation at two university hospitals in Germany. Donor sites on the upper legs were covered with a moist silicone-coated dressing. Oleogel-S10 ointment containing 10% birch bark extract was randomly applied to the distal or proximal half of the wound, with the other half serving as an intraindividual control, for 14 days after the skin graft surgery. The primary efficacy variable was faster reepithelialization as determined from macrophotographs by independent, blinded experts. Twenty-four patients were randomized and completed the trial. After the 14-day test period, the planned interim analysis revealed a highly significant (p &lt; 0.0001) superiority of Oleogel-S10 in the primary efficacy variable and the trial was terminated early due to ethical concerns. The treatment side was also better reepithelialized and more similar to normal skin after 3 months. In conclusion, Oleogel-S10 significantly accelerated reepithelialization at split-thickness skin graft donor sites. Treatment with Oleogel-S10 was safe and well tolerated. i 2014 S. Karger AG, Basel
DOI: 10.6084/m9.figshare.5126008.v1
title: Supplementary Material for: Altered Bacterial Profiles in Saliva from Adults with Caries Lesions: A Case-Cohort Study

description: The aim of this study was to learn whether presence of caries in an adult population was associated with a salivary bacterial profile different from that of individuals without untreated caries. Stimulated saliva samples from 621 participants of the Danish Health Examination Survey were analyzed using the Human Oral Microbe Identification Microarray technology. Samples from 174 individuals with dental caries and 447 from a control cohort were compared using frequency and levels of identified bacterial taxa/clusters as endpoints. Differences at taxon/cluster level were analyzed using Mann-Whitney's test with Benjamini-Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles. A reduced bacterial diversity was observed in samples from subjects with dental caries. Five bacterial taxa (<i>Veillonella parvula</i>, <i>Veillonella atypica</i>, <i>Megasphaera micronuciformis</i>, <i>Fusobacterium periodontium</i> and <i>Achromobacter xylosoxidans</i>) and one bacterial cluster (<i>Leptotrichia sp. clones C3MKM102 and GT018</i>_ot417/462) were less frequently found in the caries group (adjusted p value &lt;0.01) while two bacterial taxa (<i>Solobacterium moorei</i> and <i>Streptococcus salivarius</i>) and three bacterial clusters (<i>Streptococcus parasanguinis I and II and sp. clone BE024</i>_ot057/411/721, <i>Streptococcus parasanguinis I and II and sinensis</i>_ot411/721/767, <i>Streptococcus salivarius and sp. clone FO042</i>_ot067/755) were present at significantly higher levels (adjusted p value &lt;0.01). The principal component analysis displayed a marked difference in the bacterial community profiles between groups. Presence of manifest caries was associated with a reduced diversity and an altered salivary bacterial community profile. Our data support recent theories that ecological stress-induced changes of commensal microbial communities are involved in the shift from oral health to tooth decay.
DOI: 10.6084/m9.figshare.5126011.v1
title: Supplementary Material for: Development and Three-Dimensional Morphology of the Zygomaticotemporal Suture in Primate Skulls

description: Cranial sutures are an essential part of the growing skull, allowing bones to increase in size during growth, with their morphology widely believed to be dictated by the forces and displacements that they experience. The zygomaticotemporal suture in primates is located in the relatively weak zygomatic arch, and externally it appears a very simple connection. However, large forces are almost certainly transmitted across this suture, suggesting that it requires some level of stability while also allowing controlled movements under high loading. Here we examine the 2- and 3-dimensional (3D) morphology of the zygomaticotemporal suture in an ontogenetic series of <i>Macaca fascicularis </i>skulls. High resolution microcomputed tomography data sets were examined, and virtual and physical 3D replicas were created to assess both structure and general stability. The zygomaticotemporal suture is much more complex than its external appearance suggests, with interlocking facets between the adjacent zygomatic and temporal bones. It appears as if some movement is permitted across the suture in younger animals, but as they approach adulthood the complexity of the suture's interlocking bone facets reaches a level where these movements become minimal.
DOI: 10.6084/m9.figshare.5125996
title: Supplementary Material for: Skeletal Metastatic Carcinomas from the Roman Period (1st to 5th Century AD) in Hungary

description: <b><i>Objectives:</i></b> According to paleopathological records, tumors have a great antiquity. The prevalence of cancer in ancient populations might have differed from that in modern humans because of substantial differences in environmental factors, life expectancy and the availability of treatment. This study presents 3 cases of probable skeletal metastatic carcinoma from the Roman period (1st-5th century AD) in Hungary, showing the development of bone metastases of cancer without chemo- and radiotherapy. <b><i>Methods:</i></b> All skeletons were subjected to a careful macroscopic investigation, which was extended by radiological, stereo- and scanning electron microscopic analyses. <b><i>Results:</i></b> In 1 case, the mixed nature and localization of the lesions, as well as the sex and age of the individual, suggested breast cancer as the primary focus. In the other 2 cases, based on the mostly osteoblastic nature and the localization of the lesions as well as on the sex and age of the individuals, the most probable diagnostic option is prostate carcinoma with skeletal metastases. <b><i>Conclusions:</i></b> In view of the scarcity of cancer metastases that have been diagnosed in archeological specimens in general, identification of all examples of cancer in antiquity represents an important contribution both to paleopathology and to modern medicine.
DOI: 10.6084/m9.figshare.5125990
title: Supplementary Material for: MicroRNA Expression and Functional Profiles of Osteosarcoma

description: <b><i>Objective:</i></b> Osteosarcoma (OS) is the most frequent primary malignant bone tumor in children and young adults. Although the introduction of combined neoadjuvant chemotherapy has significantly prolonged survival, the outcome for OS patients showing a poor response to chemotherapy is still unfavorable. In order to develop new therapeutic approaches, elucidation of the entire molecular pathway regulating OS cell proliferation would be desirable. <b><i>Methods:</i></b> MicroRNA (miRNA) are highly conserved noncoding RNA that play important roles in the development and progression of various other cancers. Using miRNA microarrays capable of detecting a known number of 933 miRNA, 108 miRNA were found to be commonly expressed in 24 samples of OS tissue and subjected to a cell proliferation assay. <b><i>Results:</i></b> We found that inhibition of 5 let-7 family miRNA (hsa-let-7a, b, f, g and i) significantly suppressed the proliferation of OS cells. Using a quantitative shotgun proteomics approach, we also found that the let-7 family miRNA regulated the expression of vimentin and serpin H1 proteins. <b><i>Conclusions:</i></b> Our present results indicate the involvement of let-7 family miRNA in regulation of the cell proliferation as well as epithelial-mesenchymal transition of OS. Thus, let-7 family miRNA may potentially provide novel targets for the development of therapeutic strategies against OS.
DOI: 10.6084/m9.figshare.5125993
title: Supplementary Material for: Predictive Value of Thrombus Attenuation on Thin-Slice Non-Contrast CT for Persistent Occlusion after Intravenous Thrombolysis

description: <b><i>Background: </i></b>In stroke erythrocyte-rich thrombi are more sensitive to intravenous thrombolysis with recombinant tissue plasminogen activator (IV-rtPA) and have higher density on non-contrast CT (NCCT). We investigated the relationship between thrombus density and recanalization and whether persistent occlusions can be predicted by Hounsfield unit (HU) measurements. <b><i>Methods:</i></b> In 88 IV-rtPA-treated patients with intracranial ICA or MCA occluding thrombus and follow-up imaging, thrombus and contralateral vessel attenuation measurements were performed on thin-slice NCCT. Mean absolute and relative HU were compared between patients with persistent occlusion (modified Thrombolysis in Cerebral Infarction system, grade 0/1/2a) and recanalization (grade 2b/3). Univariate and multivariate (adjusted for stroke subtype, clot burden score, occlusion site and time to thrombolysis) odds ratios for persistent occlusion were calculated. Additional prognostic value for persistent occlusion was estimated by adding HU measurements to the area under the curve (AUC) of known determinants and calculating optimal cut-off values. <b><i>Results:</i></b> Patients with persistent occlusion (n = 19) had significant lower mean HU (absolute 52.2 ± 9.5, relative 1.29 ± 0.20) compared to recanalization (absolute 63.1 ± 10.7, relative 1.54 ± 0.23, both p &lt; 0.0001). Odds ratios for persistent occlusion were 3.1 (95% confidence interval, CI 1.6-6.0) univariate and 3.1 (95% CI 1.7-5.7) multivariate per 10 absolute HU decrease and 3.2 (95% CI 1.6-6.5) univariate and 4.1 (95% CI 1.8-9.1) multivariate per 0.20 relative HU decrease. Attenuation measurements significantly increased the AUC (0.67) of the known determinants to 0.84 (absolute HU) and 0.86 (relative HU). Cut-off values of &lt;56.5 absolute HU and &lt;1.38 relative HU showed optimal predictive values for persistent occlusion. <b><i>Conclusions:</i></b> Thrombus density is related to recanalization rate. Lower absolute and relative HU are independently related to persistent occlusion and HU measurements significantly increase discriminative performances of known recanalization determinants.
DOI: 10.6084/m9.figshare.5126002.v1
title: Supplementary Material for: The Integrative Management of Treatment-Resistant Depression: A Comprehensive Review and Perspectives

description: <b><i>Background:</i></b> Major depressive disorder is a prevalent and disabling illness. Notwithstanding numerous advances in the pharmacological treatment of depression, approximately 70% of patients do not remit after first-line antidepressant treatment. <b><i>Methods:</i></b> The MEDLINE/PubMed, EMBASE and ClinicalTrials.gov electronic databases were searched from inception to October 1, 2013, for randomized controlled trials (RCT), relevant open-label trials, meta-analyses and ongoing trials of pharmacological and psychotherapeutic approaches to treatment-resistant depression (TRD). <b><i>Results:</i></b> Switching to a different antidepressant is a useful option following nonresponse to a first-line agent. Although widely used in clinical practice, there is limited evidence to support antidepressant combination for TRD. Notwithstanding evidence for lithium or T<sub>3</sub> augmentation to be successful in TRD, most studies were carried out when participants were treated with tricyclic antidepressants (TCA). Of the available strategies to augment the response to new-generation antidepressants, the use of some atypical antipsychotics is best supported by evidence. Several novel therapeutic options are currently discussed. Evidence suggests that cognitive therapy (CT) is an effective strategy for TRD. <b><i>Conclusions:</i></b> The success of switching to a different antidepressant following a first-line agent is supported by evidence, but there is limited evidence for effective combination strategies. Lithium and T<sub>3</sub> augmentation of TCA have the strongest evidence base for successful treatment of TRD. The use of augmentation of newer-generation antidepressants with atypical antipsychotics is supported by a growing evidence base. Current evidence supports CT as an effective strategy for TRD. There is a need for additional large-scale RCT of TRD. The development of new antidepressants targeting novel pathways opens a promising perspective for the management of TRD.
DOI: 10.6084/m9.figshare.5125984
title: Supplementary Material for: Age-Related Differences in Quality of Standing Balance Using a Composite Score

description: <b><i>Background:</i></b> Age-related differences in standing balance are not detected by testing the ability to maintain balance. Quality of standing balance might be more sensitive to detect age-related differences. <b><i>Objective:</i></b> To study age-related differences in quality of standing balance, center of pressure (CoP) movement was evaluated using a wide range of CoP parameters in several standing conditions in healthy young and old participants. <b><i>Methods:</i></b> In 35 healthy young (18-30 years) and 75 healthy old (70-80 years) participants, CoP movement was assessed in eight standing conditions on a force plate, including side-by-side, one-leg, semi-tandem and tandem stance, both with eyes open and eyes closed. Direction-specific CoP composite scores were calculated from standardized single CoP parameters (mean amplitude, amplitude variability, mean velocity, velocity variability and range) in anterior-posterior (AP) and medial-lateral (ML) direction. Linear regression analysis was used to detect age-related differences in single CoP parameters and composite scores - adjusted for gender, height and weight. <b><i>Results:</i></b> Overall, single CoP parameters were higher in old compared to young participants, but no single CoP parameter consistently demonstrated the largest effect size for all standing conditions. Age-related differences were demonstrated for CoP composite scores in AP direction (tandem eyes open; semi-tandem eyes closed; p &lt; 0.001). CoP composite scores in ML direction were consistently higher for all standing conditions in old compared to young participants (p &lt; 0.001). <b><i>Conclusion:</i></b> CoP composite scores in ML direction were the most consistent parameters to detect age-related differences in quality of standing balance in healthy participants and might be of clinical value to detect subtle changes in quality of standing balance.