DOI: 10.5061/dryad.1rn8pk129
title: Data from: Effects of Cs on temperature response of C. borealis pyloric rhythm

description: Motor systems operate over a range of frequencies and relative timing
 (phase). We studied the contribution of the hyperpolarization-activated
 inward current (Ih) to frequency and phase in the pyloric rhythm of the
 stomatogastric ganglion (STG) of the crab, Cancer borealis as temperature
 was altered from 11°C to 21°C. Under control conditions, the frequency of
 the rhythm increased monotonically with temperature, while the phases of
 the pyloric dilator (PD), lateral pyloric (LP), and pyloric (PY) neurons
 remained constant. When we blocked Ih with cesium (Cs+) PD offset, LP
 onset, and LP offset were all phase advanced in Cs+ at 11°C, and the
 latter two further advanced as temperature increased. In Cs+ the steady
 state increase in pyloric frequency with temperature diminished and the
 Q10 of the pyloric frequency dropped from ~1.75 to ~1.35. Unexpectedly in
 Cs+, the frequency displayed non-monotonic dynamics during temperature
 transitions; the frequency initially dropped as temperature increased,
 then rose once temperature stabilized, creating a characteristic “jag”.
 Interestingly, these jags were still present during temperature
 transitions in Cs+ when the pacemaker was isolated by picrotoxin, although
 the temperature-induced change in frequency recovered to control levels.
 Overall, these data suggest that Ih plays an important role in the ability
 of this circuit to produce smooth transitory responses and persistent
 frequency increases by different mechanisms during temperature
 fluctuations.
description: The data consist largely of the spike times for various pyloric
 neurons recorded extra or intracellularly. These data were used to
 calculate feartures like frequency of the rhythm and phase of the
 components under various conditions. 
description: # Data from: Effects of Cs on temperature response of *C. borealis*
 pyloric rhythm
 [https://doi.org/10.5061/dryad.1rn8pk129](https://doi.org/10.5061/dryad.1rn8pk129) ## Description of the data and file structure Voltage Clamp Data: Contained in VoltageClampData.mat. VoltageClampData has 10 structures, one for each experiment. Each structure has 5 structures. ConditionDescriptions: describes the conditions of the trials in a given experiment. This includes information on the cell type (PD, LP), approximate temperature, and any Cs and/or other channel blockers like TTX or PTX. ConditionKey: Tells which rows of Voltage and Current correspond to which Condition. E.g., a "4" in position 6 says that the 6th row of Voltage and current has the 4th condition in ConditionDescriptions. Time: Index of time in seconds for every trial. Voltage: Trials x steps x time for the voltage. Each trial consisted of multiple voltage steps, usually in the range -60:-10:-120. Current: Same format as voltage but what the current injected was for each trial and step. Identified Spike and Temperature Data: In the format [Experiment Number]_SpikeAndTemp.mat. A condensed format of data analyzed for this paper. Each file contains a variable for the spike times of any relevant cells (PD, LP, PY), a subsampled array for temperature ("Tempsub") and time ("x_sub") as the original data was sampled at 10kHz. "Timings" contains the indexes for when a condition (e.g. Saline or Cs) was started and finished. "fileTimes" give the actual time that a file started. So if in Timings condition Saline started at "13", you would go to fileTimes(13+1) to find the actual time in the experiment (and thus appropriate spiking data) for that condition. The +1 is because the filing system index starts at 0 and the matlab indexing starts at 1. SummaryStatsitics.mat: Contains the originally calculated Variables for features like Q10, PDOFF Phase, burst frequency etc across the 21 experiments used in all Figs except 5. Also contains "ControlTable" which has the information used in figure 1E,F with regards to the double saline controls. There is also a like version for the PTX experimental data. Sawtooth.mat: Contains the information used for figure 3 regarding the probability of Decreasing frequency while temperature increasing "DecFreq" with row 1 being saline and row 2 being Cs. Same format for probability of Increasing Frequency while temperature stable "IncFreqTempZCnt". "files" says which experiment corresponds to which column of DecFre eg. There is also a like version for the PTX experimental data. LPAdcanceSamePeriod: Contains the data for the matched-period samples. "file" says which files were used. "meanFreq" is the frequency for the matched samples on a experiment by experiment basis (columns=experiment, row1=Saline, row2=Cs). Remaining variables are as named. Condensed Raw data: In the format Raw_Expt_[Expteriment Number]. Contains the Voltage data for the nerves in "nerve_names" in "whole_traces" ## Code/Software MakePaperFigures_Clean can be run with the data provided to make figures (prior to formatting in Illustrator) used in the paper. It is divided by Figure. plot_trace_ext_TwoCol is one of many verions of code that will take a Raw_Expt_[Experiment Number] data and plot small snippets of the voltage traces. plot_trace_examples_Temp: another version of code that takes raw data and plots a snippet. This one also plots the temperature and instantaneous frequency along with the snippet. extractBurstFrequency_Final: Code that takes the spiketimes from SpikeAndTemp data and returns information about the bursting. See file for more details. plot_trace_examples_EC3: another version of code that takes raw data and plots a snippet. This takes 3 extracellular traces. plot_trace_examples_ext_1trace: another version of code that takes raw data and plots a snippet. plot_trace_examples_IC_EC: another version of code that takes raw data and plots a snippet. ProbabilityUpDown: uses the files specified in Sawtooth.mat to calculate the probabilities of decreasing frequency during increasing temp and increasing frequency during stable temp. subplottight: utility code used in formatting some of the plot_trace_example codes. Phase_Calc: Code to calculate phase based on spike times of PD and cell of interest. CalculateFrequencyPhaseEtc: Given a particular experiment will calculate the variables found in SummaryStatistics like phase, frequency, etc based on temperature. # **Version Changes:** **August 8, 2024:** Added the specific data used to make the Figure 1--Figure Supplement 1 figure in "VoltageClampTraceData.mat". This object has one structure per experiment used in the figure. Each experimental structure contains a 1x2 structure. Row 1 is saline, row 2 is Cs. fileNames contains the original file name, Cell_Temp says which cell was being recorded from and what approximate temperature. Time, Voltage, and Current contain the appropriate measurements for each condition. MakePaperFiguresClean.m has been changed to include the new experiments in the calculations of Ih reduction as well as the code to generate the figure. VoltageClampData.mat has also been altered to include the additional experiments.