cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.6600144
title: Exogenous oxidative stressors elicit differing age and sex effects

description: Data and scripts for the manuscript entitled "Exogenous oxidative stressors elicit differing age and sex effects" submitted to <em>Experimental Gerontology. </em>
DOI: 10.5281/zenodo.5597511
title: Exogenous oxidative stressors elicit differing age and sex effects

description: Data and scripts for the manuscript entitled "Exogenous oxidative stressors elicit differing age and sex effects" submitted to <em>Experimental Gerontology. </em>
DOI: 10.6084/m9.figshare.19944730.v1
title: Trypsin inhibitor in Enterolobium contortisiliquum calli grown in the presence of plant growth regulators

description: Abstract The objective of this work was to investigate the effect of plant growth regulators (PGR) on the induction of calli from cotyledons of Enterolobium contortisiliquum grown in vitro, as well as to evaluate the inhibitory activity of trypsin in these calli. Cotyledons of E. contortisiliquum were cultivated in culture medium supplemented with dichlorophenoxyacetic acid (2,4-D), picloram (PIC), kinetin (KIN), and 6-benzylaminopurine (BAP). Callus mass, inhibitory activity of trypsin, morphogenetic and cytochemical observations, and biological activity were measured after 60 days. The Pareto diagram showed that only 0.5 mg L-1 PIC influenced the increase of fresh mass, whereas 0.5 mg L-1 BAP influenced dry mass. The principal component analysis plot showed the formation of two clear clusters. The inhibitory activity of trypsin is achieved at a higher intensity in the treatment with 0.5 mg L-1 2,4-D + 2.0 mg L-1 PIC + 0.5 mg L-1 KIN + 2.0 mg L-1 BAP. The combination of PGR at different rates causes an intense callus formation. Low concentrations of PGR result in a higher fresh mass, dry mass, and biological activity. The use of PGR is efficient in obtaining E. contortisiliquum calli with a satisfactory inhibitory activity of trypsin.
DOI: 10.6084/m9.figshare.19944730
title: Trypsin inhibitor in Enterolobium contortisiliquum calli grown in the presence of plant growth regulators

description: Abstract The objective of this work was to investigate the effect of plant growth regulators (PGR) on the induction of calli from cotyledons of Enterolobium contortisiliquum grown in vitro, as well as to evaluate the inhibitory activity of trypsin in these calli. Cotyledons of E. contortisiliquum were cultivated in culture medium supplemented with dichlorophenoxyacetic acid (2,4-D), picloram (PIC), kinetin (KIN), and 6-benzylaminopurine (BAP). Callus mass, inhibitory activity of trypsin, morphogenetic and cytochemical observations, and biological activity were measured after 60 days. The Pareto diagram showed that only 0.5 mg L-1 PIC influenced the increase of fresh mass, whereas 0.5 mg L-1 BAP influenced dry mass. The principal component analysis plot showed the formation of two clear clusters. The inhibitory activity of trypsin is achieved at a higher intensity in the treatment with 0.5 mg L-1 2,4-D + 2.0 mg L-1 PIC + 0.5 mg L-1 KIN + 2.0 mg L-1 BAP. The combination of PGR at different rates causes an intense callus formation. Low concentrations of PGR result in a higher fresh mass, dry mass, and biological activity. The use of PGR is efficient in obtaining E. contortisiliquum calli with a satisfactory inhibitory activity of trypsin.
DOI: 10.6084/m9.figshare.19944734
title: Nutritional demand and nutrient export by modern cultivars of common bean

description: Abstract The objective of this work was to quantify the extraction and export of nutrients by common bean (Phaseolus vulgaris) cultivars from different commercial groups and with different growth habits. The experiment was conducted using four common bean cultivars: TAA Gol, BRS FC104, IPR Tuiuiú, and TAA Dama. Total dry matter (DM) and nutrient accumulation in the V4 (with four, six, and eight three-leaflet leaves), R5, R7, R8, and R9 stages was determined, as well as grain yield and nutrient export. Differences were observed both in nutrient uptake and in DM and nutrient accumulation among the cultivars in the initial stages of development (V4 with six and eight three-leaflet leaves). The TAA Gol cultivar showed a greater accumulation of almost all nutrients in these two initial stages. In R7, the cultivar that had the greatest DM and nutrient accumulation was TAA Dama, but, from R8 onwards, there were no significant differences. Although nutrient export is similar among the evaluated cultivars, a lower percentage is translocated to the grain in TAA Dama.
DOI: 10.6084/m9.figshare.19944734.v1
title: Nutritional demand and nutrient export by modern cultivars of common bean

description: Abstract The objective of this work was to quantify the extraction and export of nutrients by common bean (Phaseolus vulgaris) cultivars from different commercial groups and with different growth habits. The experiment was conducted using four common bean cultivars: TAA Gol, BRS FC104, IPR Tuiuiú, and TAA Dama. Total dry matter (DM) and nutrient accumulation in the V4 (with four, six, and eight three-leaflet leaves), R5, R7, R8, and R9 stages was determined, as well as grain yield and nutrient export. Differences were observed both in nutrient uptake and in DM and nutrient accumulation among the cultivars in the initial stages of development (V4 with six and eight three-leaflet leaves). The TAA Gol cultivar showed a greater accumulation of almost all nutrients in these two initial stages. In R7, the cultivar that had the greatest DM and nutrient accumulation was TAA Dama, but, from R8 onwards, there were no significant differences. Although nutrient export is similar among the evaluated cultivars, a lower percentage is translocated to the grain in TAA Dama.
DOI: 10.6084/m9.figshare.19944736
title: Physiological quality of corn seeds treated with insecticides and stored at different temperatures

description: Abstract The objective of this work was to evaluate the effect of the industrial treatment with insecticides on the physiological quality of corn (Zea mays) seeds during storage at different temperatures. Seeds of the BM 950 PRO3 and BM 709 PRO2 hybrids were subjected to industrial treatment with insecticides based on chlorantraniliprole, cyantraniliprole, and clothianidin, as well as to a control, and stored at different temperatures (10, 20, and 30°C) for 0, 90, 180, 270, and 360 days, in a 4x3x5 factorial arrangement for each hybrid, in a completely randomized design. To evaluate the quality of the seeds, tests of moisture content, germination, seedling emergence, accelerated aging, and cold were performed. For the seeds of both hybrids, germination is preserved at minimum commercialization standards for up to 360 days of storage, regardless of the insecticide or storage temperature. The temperature of 10°C preserves seed vigor and minimizes the negative effects caused by the insecticides during storage. At 20 and 30°C, vigor is cumulatively impaired as storage is extended, mainly at 30°C. Chlorantraniliprole results in a greater preservation of seed vigor regardless of storage temperature, whereas clothianidin provides a greater loss of vigor in industrially treated corn seeds, especially when stored at 30°C.
DOI: 10.6084/m9.figshare.19944736.v1
title: Physiological quality of corn seeds treated with insecticides and stored at different temperatures

description: Abstract The objective of this work was to evaluate the effect of the industrial treatment with insecticides on the physiological quality of corn (Zea mays) seeds during storage at different temperatures. Seeds of the BM 950 PRO3 and BM 709 PRO2 hybrids were subjected to industrial treatment with insecticides based on chlorantraniliprole, cyantraniliprole, and clothianidin, as well as to a control, and stored at different temperatures (10, 20, and 30°C) for 0, 90, 180, 270, and 360 days, in a 4x3x5 factorial arrangement for each hybrid, in a completely randomized design. To evaluate the quality of the seeds, tests of moisture content, germination, seedling emergence, accelerated aging, and cold were performed. For the seeds of both hybrids, germination is preserved at minimum commercialization standards for up to 360 days of storage, regardless of the insecticide or storage temperature. The temperature of 10°C preserves seed vigor and minimizes the negative effects caused by the insecticides during storage. At 20 and 30°C, vigor is cumulatively impaired as storage is extended, mainly at 30°C. Chlorantraniliprole results in a greater preservation of seed vigor regardless of storage temperature, whereas clothianidin provides a greater loss of vigor in industrially treated corn seeds, especially when stored at 30°C.
DOI: 10.6084/m9.figshare.19944737.v1
title: Structural analysis of soybean pods and seeds subjected to weathering deterioration in pre-harvest

description: Abstract The objective of this work was to analyze structurally the pods and seed coats of soybean (Glycine max) cultivars, as well as to determine the quality of seeds when subjected to deterioration by weathering in pre-harvest. A 7×3 factorial arrangement was used – seven cultivars and three volumes of simulated rainfall (0, 54, and 162 mm). Exposure to rainfall was simulated in the R8 phenological stage. Then, the plants were taken to a greenhouse, where they were kept until the time of collection of pods and seeds. After collection, the following evaluations were carried out: tetrazolium test, lignin content in pods and seed coats, and structural analysis of pods (exocarp, mesocarp, and endocarp thickness) and seed coats (epidermis, hypodermis, and parenchymal-cell thickness). Pre-harvest rainfall of 54 and 162 mm reduces the quality of soybean seeds; however, the response to deterioration by weathering differs according to the cultivar. The tolerance to all simulated rainfall was greater for BRSMT Pintado, BRS Jiripoca, and M8210IPRO and lower for BRS 1010IPRO. Pods with a greater thickness of the exocarp, mesocarp, and endocarp and a high lignin content show greater resistance to weathering deterioration and seeds with greater vigor and viability. The thickness of the hypodermis of the testa is related to resistance to weathering deterioration and to the obtainment of high-quality seeds.
DOI: 10.6084/m9.figshare.19944737
title: Structural analysis of soybean pods and seeds subjected to weathering deterioration in pre-harvest

description: Abstract The objective of this work was to analyze structurally the pods and seed coats of soybean (Glycine max) cultivars, as well as to determine the quality of seeds when subjected to deterioration by weathering in pre-harvest. A 7×3 factorial arrangement was used – seven cultivars and three volumes of simulated rainfall (0, 54, and 162 mm). Exposure to rainfall was simulated in the R8 phenological stage. Then, the plants were taken to a greenhouse, where they were kept until the time of collection of pods and seeds. After collection, the following evaluations were carried out: tetrazolium test, lignin content in pods and seed coats, and structural analysis of pods (exocarp, mesocarp, and endocarp thickness) and seed coats (epidermis, hypodermis, and parenchymal-cell thickness). Pre-harvest rainfall of 54 and 162 mm reduces the quality of soybean seeds; however, the response to deterioration by weathering differs according to the cultivar. The tolerance to all simulated rainfall was greater for BRSMT Pintado, BRS Jiripoca, and M8210IPRO and lower for BRS 1010IPRO. Pods with a greater thickness of the exocarp, mesocarp, and endocarp and a high lignin content show greater resistance to weathering deterioration and seeds with greater vigor and viability. The thickness of the hypodermis of the testa is related to resistance to weathering deterioration and to the obtainment of high-quality seeds.
DOI: 10.6084/m9.figshare.19944739
title: MORPHOLOGICAL CHARACTERISTICS AND GENETIC VARIABILITY OF ARATICUM USING RAPD AND MICROSATELLITES MARKERS

description: ABSTRACTThis study aimed to evaluate the genetic variability of the araticum collections from Embrapa Cerrados and others regions near to the Federal District, using RAPD and microsatellites markers as well as morphological analysis. Young leaves of 18 araticum accessions were collected and used for genomic DNA extraction. Genomic DNA samples from each accession were amplified for obtaining RAPD and microsatellites markers. Morphological analysis were performed using 23 characteristics of each araticum accession. Genetic dissimilarities among the 18 araticum accessions showed high genetic variability among them and cluster analysis led to the formation of three similarity groups. It was observed low genetic dissimilarities among accessions from Embrapa Cerrados and high genetic dissimilarities among accessions from others regions. These accessions are important as genetic variability sources to enlarge the current Embrapa Cerrados collection and for future studies of morphological and agronomic germplasm characterization.
DOI: 10.6084/m9.figshare.19944739.v1
title: MORPHOLOGICAL CHARACTERISTICS AND GENETIC VARIABILITY OF ARATICUM USING RAPD AND MICROSATELLITES MARKERS

description: ABSTRACTThis study aimed to evaluate the genetic variability of the araticum collections from Embrapa Cerrados and others regions near to the Federal District, using RAPD and microsatellites markers as well as morphological analysis. Young leaves of 18 araticum accessions were collected and used for genomic DNA extraction. Genomic DNA samples from each accession were amplified for obtaining RAPD and microsatellites markers. Morphological analysis were performed using 23 characteristics of each araticum accession. Genetic dissimilarities among the 18 araticum accessions showed high genetic variability among them and cluster analysis led to the formation of three similarity groups. It was observed low genetic dissimilarities among accessions from Embrapa Cerrados and high genetic dissimilarities among accessions from others regions. These accessions are important as genetic variability sources to enlarge the current Embrapa Cerrados collection and for future studies of morphological and agronomic germplasm characterization.
DOI: 10.6084/m9.figshare.19944742
title: DRY MATTER AND NUTRIENT ACCUMULATION IN GRAFTED PEAR SEEDLINGS IN HYDROPONIC SYSTEM

description: ABSTRACTThe production of grafted seedlings of pear and peach trees in hydroponics is recent and has been becoming a promising propagation method with a significant reduction in the seedling production time. This study was conducted in order to determine the dry matter of plant parts and the accumulation of macro and micronutrients by pear graftedseedlings in hydroponic conditions. It was used a completely randomized factorial scheme 3 x 3 with four replications was. Each replication was made up of two plants (one in each tube), amounting to 72 plants. The factors under study were: three cultivars (Triunfo, Tenra and Cascatense) and three sorts of grafting (bench grafting - BP, inverted T budding -BT and cleft grafting -GF). The nutrient accumulation in pear seedlings, regardless of the cultivar, settled in the following order for macronutrients: N&gt;Mg&gt;K&gt;Ca&gt;P&gt;S; and micronutrients: Fe&gt;Mn&gt;B&gt;Zn&gt;Cu.
DOI: 10.6084/m9.figshare.19944742.v1
title: DRY MATTER AND NUTRIENT ACCUMULATION IN GRAFTED PEAR SEEDLINGS IN HYDROPONIC SYSTEM

description: ABSTRACTThe production of grafted seedlings of pear and peach trees in hydroponics is recent and has been becoming a promising propagation method with a significant reduction in the seedling production time. This study was conducted in order to determine the dry matter of plant parts and the accumulation of macro and micronutrients by pear graftedseedlings in hydroponic conditions. It was used a completely randomized factorial scheme 3 x 3 with four replications was. Each replication was made up of two plants (one in each tube), amounting to 72 plants. The factors under study were: three cultivars (Triunfo, Tenra and Cascatense) and three sorts of grafting (bench grafting - BP, inverted T budding -BT and cleft grafting -GF). The nutrient accumulation in pear seedlings, regardless of the cultivar, settled in the following order for macronutrients: N&gt;Mg&gt;K&gt;Ca&gt;P&gt;S; and micronutrients: Fe&gt;Mn&gt;B&gt;Zn&gt;Cu.
DOI: 10.6084/m9.figshare.19944743
title: GENETIC VARIABILITY OF PARENTALS AND INTER AND INTERSPECIFIC F1 POPULATIONS OF Physalis peruviana L. AND P. floridana Rydb.

description: ABSTRACTCape gooseberry, Physalis peruviana, is an Andean fruit of great importance for export markets; its main limitation for production in Colombia is the vascular wilt caused by Fusarium oxysporum. The present study proposed the generation of F1 populations between contrasting pathogen response parents and their evaluation at molecular level to support knowledge and use of the species genetics resources. To do this, four genotypes of P. peruviana, and one of the related species P. floridana, were characterized at morphological level using 34 qualitative and 20 quantitative variables and at molecular level using 328 COSII and 154 IRGs markers. The genotypes were used as parents for generation and molecular characterization of F1 populations. Quantitative variables were able to distinguish the species P. floridana and P. peruviana as well as cultivated and wild genotypes within P. peruviana. One hundred percent viability was found in intraspecific F1 crosses and 50% in interspecific crosses, remaining viable only when P. floridana was the receptor of pollen. Molecular characterization did not identify polymorphisms within the P. peruviana but between P. floridana and P. peruviana. An F1 population of 51 individuals generated between the species a total of 127 alleles with an average of 3.18 per locus, a PIC of 0.358 and high values of heterozygosity (Ho: 0.737 and He: 0.449). PCA and cluster analysis allowed discrimination of the F1 population in three groups, more similar to the P. floridana parental. This was reflected by a 75% Mendelian distortion favored by the presence of 63.75% maternal alleles. The study provides insights into Cape gooseberry crossability and the genetic variability of parental genotypes and F1 populations.
DOI: 10.6084/m9.figshare.19944743.v1
title: GENETIC VARIABILITY OF PARENTALS AND INTER AND INTERSPECIFIC F1 POPULATIONS OF Physalis peruviana L. AND P. floridana Rydb.

description: ABSTRACTCape gooseberry, Physalis peruviana, is an Andean fruit of great importance for export markets; its main limitation for production in Colombia is the vascular wilt caused by Fusarium oxysporum. The present study proposed the generation of F1 populations between contrasting pathogen response parents and their evaluation at molecular level to support knowledge and use of the species genetics resources. To do this, four genotypes of P. peruviana, and one of the related species P. floridana, were characterized at morphological level using 34 qualitative and 20 quantitative variables and at molecular level using 328 COSII and 154 IRGs markers. The genotypes were used as parents for generation and molecular characterization of F1 populations. Quantitative variables were able to distinguish the species P. floridana and P. peruviana as well as cultivated and wild genotypes within P. peruviana. One hundred percent viability was found in intraspecific F1 crosses and 50% in interspecific crosses, remaining viable only when P. floridana was the receptor of pollen. Molecular characterization did not identify polymorphisms within the P. peruviana but between P. floridana and P. peruviana. An F1 population of 51 individuals generated between the species a total of 127 alleles with an average of 3.18 per locus, a PIC of 0.358 and high values of heterozygosity (Ho: 0.737 and He: 0.449). PCA and cluster analysis allowed discrimination of the F1 population in three groups, more similar to the P. floridana parental. This was reflected by a 75% Mendelian distortion favored by the presence of 63.75% maternal alleles. The study provides insights into Cape gooseberry crossability and the genetic variability of parental genotypes and F1 populations.
DOI: 10.6084/m9.figshare.19944745.v1
title: DRIS NORMS AND CRITICAL LEAF NUTRIENT LEVELS FOR ‘NIAGARA ROSADA’ GRAPE IN JUNDIAÍ REGION, SÃO PAULO (BRASIL)

description: ABSTRACTPreliminary Diagnosis and Recommendation Integrated System (DRIS) norms and critical leaf nutrient levels (CLN) for ‘Niagara Rosada’ grape grafted on the rootstock ‘IAC 766’ growing in the region of Jundiaí, State of São Paulo (Brazil) are presented. DRIS norms and CLN were established from a data bank of leaf nutrient concentration(N, P, K, Ca and Mg) and fruit yield with 116 records. The data bank gets information from fertilizationexperiments and from a field survey comprising four crop cycles. The data were divided into high-yielding (&gt;26 t/ha) and low-yielding (&lt;26 t/ha) subpopulations and norms were computed using standard DRIS procedures. Even with the diversity of cropping conditions, the relationship between the nutrient balance index from DRIS and fruit yield was highly significant (R2 = 0.48, p&lt;0.0001), indicating that part of the variation in productivity of vineyards can be credited to nutritional status of the plants. The CLNs were derived using multiple linear regressions relating to the foliar nutrient concentration with DRIS indices of all nutrients. The CLNs for N, P, K, Ca, and Mg were 38.0, 3.0, 11.4, 13.7, and 3.5 g/ kg, respectively.
DOI: 10.6084/m9.figshare.19944745
title: DRIS NORMS AND CRITICAL LEAF NUTRIENT LEVELS FOR ‘NIAGARA ROSADA’ GRAPE IN JUNDIAÍ REGION, SÃO PAULO (BRASIL)

description: ABSTRACTPreliminary Diagnosis and Recommendation Integrated System (DRIS) norms and critical leaf nutrient levels (CLN) for ‘Niagara Rosada’ grape grafted on the rootstock ‘IAC 766’ growing in the region of Jundiaí, State of São Paulo (Brazil) are presented. DRIS norms and CLN were established from a data bank of leaf nutrient concentration(N, P, K, Ca and Mg) and fruit yield with 116 records. The data bank gets information from fertilizationexperiments and from a field survey comprising four crop cycles. The data were divided into high-yielding (&gt;26 t/ha) and low-yielding (&lt;26 t/ha) subpopulations and norms were computed using standard DRIS procedures. Even with the diversity of cropping conditions, the relationship between the nutrient balance index from DRIS and fruit yield was highly significant (R2 = 0.48, p&lt;0.0001), indicating that part of the variation in productivity of vineyards can be credited to nutritional status of the plants. The CLNs were derived using multiple linear regressions relating to the foliar nutrient concentration with DRIS indices of all nutrients. The CLNs for N, P, K, Ca, and Mg were 38.0, 3.0, 11.4, 13.7, and 3.5 g/ kg, respectively.
DOI: 10.6084/m9.figshare.19944746.v1
title: STARCH AND SODIUM HIPOCHLORITE ON IN VITRO ROOTING OF PINEAPPLE ‘GOLD’ AND ITS EFFECTS ON ACCLIMATIZATION

description: This study aimed to evaluate the total or partial replacement of agar by corn starch and cassava starch and physical sterilization in autoclave for chemical sterilization with sodium hypochlorite (NaClO) in development of pineapple ‘Gold’ plantlets. The gelling agents used were: M1: agar (6.0 g L-1); M2: corn starch (60.0 g L-1); M3: agar (3.0 g L-1) + corn starch (30.0 g L-1) and M4: agar (3.0 g L-1) + cassava starch (30.0 g L-1). The sterilizations were made by autoclaving at 121°C or by using the solutions of NaClO at the concentrations of 0,003% to rinse the glassware and 0,0003% for addition to the culture medium. After one month of in vitro cultivation, it was observed no negative influence of NaClO and gelling agents in the rooting in vitro of shoots for most parameters evaluated. The plantlets derived from in vitro phase were taken to acclimatization in greenhouse for 90 days. During the acclimatization, the plantlets derived from treatments with chemical sterilization did not differ from the ones derived of autoclaved media to any parameter evaluated, whereas the combination agar + corn starch provided inferior results among gelling agents.