cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.a2wwx8
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Gomphonema angusta (Rabenhorst)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((20.7576 -24.3688,20.529 -24.0715,20.509 -24.0715,20.509 -28.5786,25.5395 -28.5786,25.5395 -24.0715,25.1306 -24.0715,24.9333 -24.4571,23.8962 -24.2467,22.8065 -24.295,22.3719 -24.7207,22.2323 -25.547,22.0387 -25.7188,21.7833 -25.7784,21.5388 -25.7113,21.3553 -25.5415,20.7576 -24.3688))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044501-250525065834625/datasets/export for details.
DOI: 10.6084/m9.figshare.29309768
title: Figure 6C& D

description: A. Schematic representation of the experiment methodology for this figure. B. Supernatants from infected MRC-5 cells expressing either vector or MyD88 at an MOI of 0.05, with TB40/E-mCh, were collected every three days for 15 days and filtered through 0.2 mm disc filter. The filtered supernatants from each day were then added to another monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). Cells were monitored for virus spread by imaging every three days. C. Supernatants from infected MRC-5 cells expressing either vector or MyD88 were collected at 7 dpi and filtered through a 0.2 mm disc filter. They were then either untreated or denatured by boiling at 95° C before storage. The un-boiled and boiled supernatants were added on another monolayer of MRC-5 infected with TB40/E-mCh at MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). The spread of the virus was monitored by imaging every three days. D. A monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 was treated with mock (UT), IFN-β (50ng/ml), or IL1-β (100ng/ml). The spread of the virus was monitored every three days by confocal imaging up to day 15. The spread of infection was quantified as the area of fluorescence using the NIS element software. Results are shown as mean ± SD. * p&lt;0.05.
DOI: 10.6084/m9.figshare.29309768.v1
title: Figure 6C& D

description: A. Schematic representation of the experiment methodology for this figure. B. Supernatants from infected MRC-5 cells expressing either vector or MyD88 at an MOI of 0.05, with TB40/E-mCh, were collected every three days for 15 days and filtered through 0.2 mm disc filter. The filtered supernatants from each day were then added to another monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). Cells were monitored for virus spread by imaging every three days. C. Supernatants from infected MRC-5 cells expressing either vector or MyD88 were collected at 7 dpi and filtered through a 0.2 mm disc filter. They were then either untreated or denatured by boiling at 95° C before storage. The un-boiled and boiled supernatants were added on another monolayer of MRC-5 infected with TB40/E-mCh at MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). The spread of the virus was monitored by imaging every three days. D. A monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 was treated with mock (UT), IFN-β (50ng/ml), or IL1-β (100ng/ml). The spread of the virus was monitored every three days by confocal imaging up to day 15. The spread of infection was quantified as the area of fluorescence using the NIS element software. Results are shown as mean ± SD. * p&lt;0.05.
DOI: 10.15468/dl.q3cxck
title: Occurrence Download

description: A dataset containing 30713 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Genista hispanica L.",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 30713 records from 282 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044502-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ewr356
title: Occurrence Download

description: A dataset containing 30314 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Allium scorodoprasum L."
 ]
}

 The dataset includes 30314 records from 382 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044500-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29309846
title: Figure 8 E, F

description: <b>A. </b>THP-1 cells were infected by spinoculation with an MOI of 20 with either nonfluorescent TB40/E, UL88-STOP, or UL88-Rev, the latter two of which express GFP driven from the IE2 promoter or were left uninfected. GFP fluorescence was measured by flow cytometry at d5 post infection. <b>B.</b> Schematic representation of the experiment methodology for <b>C</b> and <b>D</b>.<b> </b>At day 3, 2.5 x 10<sup>5</sup> THP-1, of which ~ 2.5 x 10<sup>4</sup> were HCMV-infected, were transferred onto a monolayer of ~ 2.5 x 10<sup>5</sup> HDFs and virus spread was monitored every three days by confocal imaging up to day 15. <b>C</b>. Representative images of TB40/E-GFP and UL88-STOP from day 3 and day 7 are shown. <b>D.</b> The spread of infection was quantified as the area of fluorescence using the NIS element software.<b> </b>Graph shows fold change in area of fluorescence for each virus. <b>E. </b>MRC-5 cells were infected with TB40/E-mCh or UL88-STOP-mCh virus at MOI of 0.05. Cells were harvested for RNA isolation at 7 dpi. Antiviral gene expression was profiled using a set of 84 ISGs by qPCR. Results are plotted as volcano plot depicting the modulated genes as compared between TB40/E WT HCMV and UL88-STOP HCMV infection. <b>F</b>. ISGs known to be modulated in HCMV infection are shown with their relative fold change in mRNA levels (normalized to GAPDH) between TB40/E WT and UL88-STOP virus infection. The graph shows the genes that remained unchanged (green box), downregulated (red box), or upregulated (blue box), in TB40/E WT vs UL88-STOP HCMV-infected cells. Results are from biological triplicates.
DOI: 10.6084/m9.figshare.29309846.v1
title: Figure 8 E, F

description: <b>A. </b>THP-1 cells were infected by spinoculation with an MOI of 20 with either nonfluorescent TB40/E, UL88-STOP, or UL88-Rev, the latter two of which express GFP driven from the IE2 promoter or were left uninfected. GFP fluorescence was measured by flow cytometry at d5 post infection. <b>B.</b> Schematic representation of the experiment methodology for <b>C</b> and <b>D</b>.<b> </b>At day 3, 2.5 x 10<sup>5</sup> THP-1, of which ~ 2.5 x 10<sup>4</sup> were HCMV-infected, were transferred onto a monolayer of ~ 2.5 x 10<sup>5</sup> HDFs and virus spread was monitored every three days by confocal imaging up to day 15. <b>C</b>. Representative images of TB40/E-GFP and UL88-STOP from day 3 and day 7 are shown. <b>D.</b> The spread of infection was quantified as the area of fluorescence using the NIS element software.<b> </b>Graph shows fold change in area of fluorescence for each virus. <b>E. </b>MRC-5 cells were infected with TB40/E-mCh or UL88-STOP-mCh virus at MOI of 0.05. Cells were harvested for RNA isolation at 7 dpi. Antiviral gene expression was profiled using a set of 84 ISGs by qPCR. Results are plotted as volcano plot depicting the modulated genes as compared between TB40/E WT HCMV and UL88-STOP HCMV infection. <b>F</b>. ISGs known to be modulated in HCMV infection are shown with their relative fold change in mRNA levels (normalized to GAPDH) between TB40/E WT and UL88-STOP virus infection. The graph shows the genes that remained unchanged (green box), downregulated (red box), or upregulated (blue box), in TB40/E WT vs UL88-STOP HCMV-infected cells. Results are from biological triplicates.
DOI: 10.5281/zenodo.15660408
title: Optimization of electrocoagulation for high-strength cassava starch wastewater treatment: a comparative study of steel and aluminum electrodes

description: Este dataset contiene los datos experimentales completos generados para el estudio sobre el tratamiento de aguas residuales de la industria del almidón de yuca mediante electrocoagulación. Los datos respaldan los hallazgos presentados en el manuscrito.
DOI: 10.5281/zenodo.15660409
title: Optimization of electrocoagulation for high-strength cassava starch wastewater treatment: a comparative study of steel and aluminum electrodes

description: Este dataset contiene los datos experimentales completos generados para el estudio sobre el tratamiento de aguas residuales de la industria del almidón de yuca mediante electrocoagulación. Los datos respaldan los hallazgos presentados en el manuscrito.
DOI: 10.15468/dl.4zzvff
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Crassula gemmifera Friedrich",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((15.4784 -23.4046,15.4933 -24.0715,20.509 -24.0715,20.509 -24.0455,20.0952 -23.5072,19.9968 -23.017,19.7843 -22.5405,19.1988 -21.8785,18.5972 -21.5447,17.8859 -21.3993,17.1626 -21.4805,16.5332 -21.7596,16.1257 -22.0882,15.8234 -22.4662,15.606 -22.8993,15.4784 -23.4046))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044503-250525065834625/datasets/export for details.
DOI: 10.6084/m9.figshare.29309777
title: Figure 7A

description: <b>A.</b> MRC5 cells were plated on coverslips and infected with TB40/E-mCh), UL88-STOP-mCh, or UL88-Rev-mCh at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue) and NF-KB (green), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.<b> B. </b>MRC5 cells expressing either GFP-vector or GFP-UL88 were plated on coverslips and infected with mCherry tagged WT or UL88-STOP TB40/E virus at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue), GFP (green) and NF-KB (grey), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.
DOI: 10.6084/m9.figshare.29309777.v1
title: Figure 7A

description: <b>A.</b> MRC5 cells were plated on coverslips and infected with TB40/E-mCh), UL88-STOP-mCh, or UL88-Rev-mCh at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue) and NF-KB (green), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.<b> B. </b>MRC5 cells expressing either GFP-vector or GFP-UL88 were plated on coverslips and infected with mCherry tagged WT or UL88-STOP TB40/E virus at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue), GFP (green) and NF-KB (grey), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.
DOI: 10.15468/dl.cttsnd
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is iNaturalist Research-grade Observations",
 "OccurrenceStatus is Present",
 "TaxonKey is Stenocercus rhodomelas (Boulenger, 1899)"
 ]
}

 The dataset includes 5 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044505-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29309783
title: Figure 7B

description: <b>A.</b> MRC5 cells were plated on coverslips and infected with TB40/E-mCh), UL88-STOP-mCh, or UL88-Rev-mCh at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue) and NF-KB (green), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.<b> B. </b>MRC5 cells expressing either GFP-vector or GFP-UL88 were plated on coverslips and infected with mCherry tagged WT or UL88-STOP TB40/E virus at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue), GFP (green) and NF-KB (grey), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.
DOI: 10.15468/dl.e29ajk
title: Occurrence Download

description: A dataset containing 65 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Türkiye",
 "OccurrenceStatus is Present",
 "TaxonKey is Pinus halepensis Mill."
 ]
}

 The dataset includes 65 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044506-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29309840
title: Figure 8A

description: <b>A. </b>THP-1 cells were infected by spinoculation with an MOI of 20 with either nonfluorescent TB40/E, UL88-STOP, or UL88-Rev, the latter two of which express GFP driven from the IE2 promoter or were left uninfected. GFP fluorescence was measured by flow cytometry at d5 post infection. <b>B.</b> Schematic representation of the experiment methodology for <b>C</b> and <b>D</b>.<b> </b>At day 3, 2.5 x 10<sup>5</sup> THP-1, of which ~ 2.5 x 10<sup>4</sup> were HCMV-infected, were transferred onto a monolayer of ~ 2.5 x 10<sup>5</sup> HDFs and virus spread was monitored every three days by confocal imaging up to day 15. <b>C</b>. Representative images of TB40/E-GFP and UL88-STOP from day 3 and day 7 are shown. <b>D.</b> The spread of infection was quantified as the area of fluorescence using the NIS element software.<b> </b>Graph shows fold change in area of fluorescence for each virus. <b>E. </b>MRC-5 cells were infected with TB40/E-mCh or UL88-STOP-mCh virus at MOI of 0.05. Cells were harvested for RNA isolation at 7 dpi. Antiviral gene expression was profiled using a set of 84 ISGs by qPCR. Results are plotted as volcano plot depicting the modulated genes as compared between TB40/E WT HCMV and UL88-STOP HCMV infection. <b>F</b>. ISGs known to be modulated in HCMV infection are shown with their relative fold change in mRNA levels (normalized to GAPDH) between TB40/E WT and UL88-STOP virus infection. The graph shows the genes that remained unchanged (green box), downregulated (red box), or upregulated (blue box), in TB40/E WT vs UL88-STOP HCMV-infected cells. Results are from biological triplicates.
DOI: 10.6084/m9.figshare.29309840.v1
title: Figure 8A

description: <b>A. </b>THP-1 cells were infected by spinoculation with an MOI of 20 with either nonfluorescent TB40/E, UL88-STOP, or UL88-Rev, the latter two of which express GFP driven from the IE2 promoter or were left uninfected. GFP fluorescence was measured by flow cytometry at d5 post infection. <b>B.</b> Schematic representation of the experiment methodology for <b>C</b> and <b>D</b>.<b> </b>At day 3, 2.5 x 10<sup>5</sup> THP-1, of which ~ 2.5 x 10<sup>4</sup> were HCMV-infected, were transferred onto a monolayer of ~ 2.5 x 10<sup>5</sup> HDFs and virus spread was monitored every three days by confocal imaging up to day 15. <b>C</b>. Representative images of TB40/E-GFP and UL88-STOP from day 3 and day 7 are shown. <b>D.</b> The spread of infection was quantified as the area of fluorescence using the NIS element software.<b> </b>Graph shows fold change in area of fluorescence for each virus. <b>E. </b>MRC-5 cells were infected with TB40/E-mCh or UL88-STOP-mCh virus at MOI of 0.05. Cells were harvested for RNA isolation at 7 dpi. Antiviral gene expression was profiled using a set of 84 ISGs by qPCR. Results are plotted as volcano plot depicting the modulated genes as compared between TB40/E WT HCMV and UL88-STOP HCMV infection. <b>F</b>. ISGs known to be modulated in HCMV infection are shown with their relative fold change in mRNA levels (normalized to GAPDH) between TB40/E WT and UL88-STOP virus infection. The graph shows the genes that remained unchanged (green box), downregulated (red box), or upregulated (blue box), in TB40/E WT vs UL88-STOP HCMV-infected cells. Results are from biological triplicates.
DOI: 10.15468/dl.ye8su5
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Gomphonema angusta (Rabenhorst)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((20.529 -24.0715,20.509 -24.0455,20.509 -24.0715,20.529 -24.0715))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044504-250525065834625/datasets/export for details.
DOI: 10.6084/m9.figshare.29309762.v1
title: Figure 6B

description: A. Schematic representation of the experiment methodology for this figure. B. Supernatants from infected MRC-5 cells expressing either vector or MyD88 at an MOI of 0.05, with TB40/E-mCh, were collected every three days for 15 days and filtered through 0.2 mm disc filter. The filtered supernatants from each day were then added to another monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). Cells were monitored for virus spread by imaging every three days. C. Supernatants from infected MRC-5 cells expressing either vector or MyD88 were collected at 7 dpi and filtered through a 0.2 mm disc filter. They were then either untreated or denatured by boiling at 95° C before storage. The un-boiled and boiled supernatants were added on another monolayer of MRC-5 infected with TB40/E-mCh at MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). The spread of the virus was monitored by imaging every three days. D. A monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 was treated with mock (UT), IFN-β (50ng/ml), or IL1-β (100ng/ml). The spread of the virus was monitored every three days by confocal imaging up to day 15. The spread of infection was quantified as the area of fluorescence using the NIS element software. Results are shown as mean ± SD. * p&lt;0.05.
DOI: 10.15468/dl.38d3wa
title: Occurrence Download

description: A dataset containing 160303 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "StateProvince is one of (CUNDINAMARCA/META, Cundinamarca, Cundinamarca (?), Cundinamarca / Distrito Capital, Cundinamarca Departament, Cundinamarca Prov., Cundinamarca Department, Casanare, Casanare Department, Casanare Dept., Casanaré)",
 "TaxonKey is Trochilidae"
 ]
}

 The dataset includes 160303 records from 143 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044449-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29309702.v1
title: Figure 4D

description: <b>A.</b> Lentivirus-mediated downregulation of MyD88. MRC-5 cells were transduced with control lentivirus or lentivirus encoding shRNA targeting MyD88 (shMyD88-1 and shMyD88-2) and selected using puromycin. Knockdown efficiency was assessed by western blot analysis. Blots were probed for MyD88 and p115 as loading control. <b>B. </b>The transduced and selected MRC-5 cells were infected with TB40/E-mCh virus at MOI of 0.05 and spread of the virus was monitored by confocal imaging. Representative images from day 3, 9 and 15 are shown. <b>C. </b>The spread of infection was quantified as the area of fluorescence using the NIS element software. <b>D. </b>MRC-5 cells were transduced with lentivirus from control or two different cassettes encoding the MyD88 gene (MyD88-1 and MyD88-2) and subjected to puromycin selection. Cell lysates were harvested and run on western blot to analyse MyD88 levels. Tubulin served as a loading control. <b>E. </b>The transduced and selected MRC-5 cells were infected with TB40/E-mCh virus at MOI of 0.05 and the spread of the virus was monitored every three days by confocal imaging up to day 21. Representative images from day 3, 9 and 15 are shown. <b>F. </b>The spread of infection was quantified as the area of fluorescence using the NIS element software.