cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0bk1bnf
title: CIL:39795, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Higher magnification view of the spongiome of the Contractile Vacuole Complex. The spongiome is composed of a three dimensional array of tubules that are continuous with the CV membrane. Many tubules bear peg-like elements on their cytosolic surfaces reminiscent of the decorated tubules of Paramecium. These pegs are probably V-ATPase proton pumps. TEM taken on 5/1/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j00c4v20
title: CIL:39794, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The two CV pores of a cell. An impression of the helically wound microtubules are detectable in the right pore. TEM taken on 4/21/78 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 21,750X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j0b27tmw
title: CIL:39789, Lytechinus pictus, mesenchymal cell. In Cell Image Library

description: Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. This is a high magnification image of CIL 39785. Embryo was cut to reveal blastocoel cavity. The primary mesenchyme cells have formed a syncytial ring, which is the precursor to spicule formation (developing calcareous larval skeleton).This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.
description: Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data
description: Images taken on a JEOL 35C SEM. 
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW 
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.
DOI: 10.6075/j04f1q24
title: CIL:39785, Lytechinus pictus, mesenchymal cell. In Cell Image Library

description: Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. Embryo was cut to reveal blastocoel cavity. The fertilization envelope dissolves during swimming blastula stage; therefore, the cilia and microvilli can be seen on the outer epithelial layer. The primary mesenchyme cells have formed a syncytial ring, which is the precursor to spicule formation(developing calcareous larval skeleton). A portion of the archenteron can also be seen. A high magnification view of a region of this image is available as CIL:39789.This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.
description: Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data
description: Images taken on a JEOL 35C SEM. 
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW 
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.
DOI: 10.6075/j0t72gs5
title: CIL:39743, Mus musculus, retinal rod cell. In Cell Image Library

description: TEM image of epon section of a freeze-substituted mouse retina illustrating the unusual distribution of heterochromatin in rod receptor nuclei where the compact heterochromatin is centrally located. Here, a 3D tomographic reconstruction (B) is shown of a boxed area (in A) containing mostly euchromatin. (B) shows a 4nm slice through the tomogram in which electron dense particles identified as nucleosomes are seen (arrowheads) 
 See Fig 2A,B in: C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039
description: Discs were punched from freshly dissected mouse retina, cryoprotectant added, the samples cryo-fixed using high pressure freezing using a Leica EMPACT2, freeze-substituted in acetone containing OsO4, and embedded in Epon. Sections were stained with uranyl acetate and lead citrate, and observed with a Tecnai F20 TEM, and tilt series recorded with a 2k CCD camera.
See also: C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039
description: Preparation: cryofixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: charge coupled device (CCD)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Processed
DOI: 10.5281/zenodo.17303596
title: Experimental data related to the publication: "High-throughput microstructure characterization of compositionally graded Cr-Nb-Ti-Zr complex concentrated alloys (CCA) prepared by laser directed energy deposition"

description: Data associated with the research article “High-throughput microstructure characterization of compositionally graded Cr–Nb–Ti–Zr complex concentrated alloys (CCA) prepared by laser directed energy deposition” (https://doi.org/10.1016/j.jmrt.2025.10.077).

All figures in the article were generated exclusively from these datasets using the data-processing methods described therein. High-resolution schematic, photographic, and SEM images (Figs. 1, 2, 3, 4, 6, 8, and 9) are available directly within the published article (both in PDF and online versions).

The '.csv' files containing data corresponding to Figs. 5, 7, 10, 11, 12, and 13 are labelled so that their filenames include the figure designation followed by the represented physical quantity.  For Figs. 7a, 7c, 7e, and 13, the first row indicates the material and/or experimental conditions, the second row lists the x- and y-axis titles, and the numerical data begin in the third row.  For Figs. 5, 7b, 7d, 7f, 10, 11, and 12, the first two rows describe the material and/or experimental conditions, the third row specifies the x- and y-axis titles, and the data begin in the fourth row.

The raw EDS data related to the different composition gradients are provided in open '.h5' format and are contained in the 'EDS.zip' archive.

The gradients are defined as follows (see also the VoR manuscript, page 3917); the corresponding raw files are indicated in square brackets:  - Grad 1: CrNb (bottom) → TiZr (top), [from Area 5 (z = 0 mm) to Area 35 (z = 7.5 mm)]  - Grad 2: TiZr (bottom) → CrNb (top), [from Area 10 (z = 0 mm) to Area 36 (z = 6.5 mm)]  - Grad 3: CrZr (bottom) → TiNb (top), [not analysed by EDS as it is similar to Grad 4]  - Grad 4: TiNb (bottom) → CrZr (top), [from Area 4 (z = 0 mm) to Area 19 (z = 3.75 mm)]

Experimental conditions are described in detail in the cited article.
DOI: 10.5281/zenodo.17303597
title: Experimental data related to the publication: "High-throughput microstructure characterization of compositionally graded Cr-Nb-Ti-Zr complex concentrated alloys (CCA) prepared by laser directed energy deposition"

description: Data associated with the research article “High-throughput microstructure characterization of compositionally graded Cr–Nb–Ti–Zr complex concentrated alloys (CCA) prepared by laser directed energy deposition” (https://doi.org/10.1016/j.jmrt.2025.10.077).

All figures in the article were generated exclusively from these datasets using the data-processing methods described therein. High-resolution schematic, photographic, and SEM images (Figs. 1, 2, 3, 4, 6, 8, and 9) are available directly within the published article (both in PDF and online versions).

The '.csv' files containing data corresponding to Figs. 5, 7, 10, 11, 12, and 13 are labelled so that their filenames include the figure designation followed by the represented physical quantity.  For Figs. 7a, 7c, 7e, and 13, the first row indicates the material and/or experimental conditions, the second row lists the x- and y-axis titles, and the numerical data begin in the third row.  For Figs. 5, 7b, 7d, 7f, 10, 11, and 12, the first two rows describe the material and/or experimental conditions, the third row specifies the x- and y-axis titles, and the data begin in the fourth row.

The raw EDS data related to the different composition gradients are provided in open '.h5' format and are contained in the 'EDS.zip' archive.

The gradients are defined as follows (see also the VoR manuscript, page 3917); the corresponding raw files are indicated in square brackets:  - Grad 1: CrNb (bottom) → TiZr (top), [from Area 5 (z = 0 mm) to Area 35 (z = 7.5 mm)]  - Grad 2: TiZr (bottom) → CrNb (top), [from Area 10 (z = 0 mm) to Area 36 (z = 6.5 mm)]  - Grad 3: CrZr (bottom) → TiNb (top), [not analysed by EDS as it is similar to Grad 4]  - Grad 4: TiNb (bottom) → CrZr (top), [from Area 4 (z = 0 mm) to Area 19 (z = 3.75 mm)]

Experimental conditions are described in detail in the cited article.
DOI: 10.6075/j0pc31qd
title: CIL:39742, Mus musculus, retinal rod cell. In Cell Image Library

description: Discs were punched from freshly dissected mouse retina, cryoprotectant added, the samples cryo-fixed using high pressure freezing using a Leica EMPACT2, freeze-substituted in acetone containing OsO4, and embedded in Epon. Sections were stained with uranyl acetate and lead citrate, and observed with a Tecnai F20 TEM, and tilt series recorded with a 2k CCD camera.
See also: C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039
description: Preparation: cryofixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: charge coupled device (CCD)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Processed
description: TEM image of epon section of a freeze-substituted mouse retina illustrating the unusual distribution of heterochromatin in rod receptor nuclei where the compact heterochromatin is centrally located. C = cytoplasm, EC = euchromatin, HCc = central heterochromatin, HCp = nuclear envelope-associated heterochromatin, NM = nuclear envelope.
 See Fig 1B in: C Kizilyaprak et al. 2010. In Vivo Chromatin Organization of Mouse Rod Photoreceptors Correlates with Histone Modifications. PLOS one 5:e11039
DOI: 10.6075/j0445k8k
title: CIL:39740. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: negative staining; uranyl salt
Processing history: montage
Data qualification: Processed
description: Images of negatively stained chromatin fibers prepared from tandem arrays of a nucleosome positioning sequence reconstituted with purified histones. These samples contained 61 repeats of a 197 bp DNA sequence reconstituted with core histones alone (leftmost image) plus different amounts of linker histone H5 (33%, 67%, and 100% respectively).
 See: Fig 3A in Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.
description: Reconstituted chromatin was fixed with 0.1% glutaraldehyde for 30 min, deposited on glow discharged carbon films, negatively stained with aqueous uranyl acetate, and examined with a Philips 2085 TEM operated at 80 KV.
See also: Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877
DOI: 10.6075/j0gh9h9m
title: CIL:39783, Lytechinus pictus, micromere, macromere, mesomere. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data
description: Images taken on a JEOL 35C SEM. 
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW 
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.
description: Scanning electron microscope image of Lytechinus pictus [sea urchin] embryo at the 16-cell stage. The four large macromeres are behind the four small micromeres. The eight mesomeres are behind the macromeres. Remnants of the fertilization envelope can be seen attached to some of the cells.This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.
DOI: 10.6075/j0xp7492
title: CIL:39781, Mus musculus, myofibril. In Cell Image Library

description: Transmission electron micrograph of ventricle tissue of a mouse cardiac muscle. This image shows the intercalated discs between the muscle fibers as well as many mitochondria and lipid droplets.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
description: Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.
DOI: 10.6075/j0t152zh
title: CIL:39779, Mus musculus, capillary endothelial cell, blood cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
description: Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.
description: Transmission electron micrograph of a highly magnified and cropped image of a capillary in a mouse cardiac muscle. The capillary is used mainly for the transport of nutrients and oxygen through the elaborate network of branches. Made up of endothelial cells the capillary has the ability to constrict or dilate when signaled by the body. Red blood cells average about 7.7 um in diameter and seen here as a darkly colored region in which its‚Äô sole function is to deliver oxygen throughout the tissues of the body.
DOI: 10.6075/j0dj5f0z
title: CIL:39764, Mus musculus, cardiac muscle cell, blood cell. In Cell Image Library

description: Transmission electron micrograph of a blood vessel in the atria of a mouse cardiac tissue. This image is a close up of a red blood cell in a blood vessel.
description: Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
DOI: 10.6075/j0ws8skm
title: CIL:39760, Mus musculus, cardiac muscle cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
description: Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.
description: Transmission electron micrograph of the periphery of an atria mouse cardiac muscle. This micrograph shows how rich cardiac tissue is with mitochondria reflecting its greater dependence on cellular respiration for ATP. Interspersed within the mitochondria are lipid droplets which have been found to play an important role as energy source as well. The Sarcoplasmic Reticulum consists of a consistent three-dimensional tubular system at the cells periphery and functions mainly to store large amounts of calcium ions which are sequestered until stimulated by an electrical impulse.