DOI: 10.18150/llhi81
title: The role of dialdehyde kefiran as a crosslinking agent in chitosan/kefiran-based materials with “Henola” extracts: a biocompatible strategy in wound care

description: 1. General Information- **Data Set Title**: The role of dialdehyde kefiran as a crosslinking agent in chitosan/kefiran-based materials with “Henola” extracts: a biocompatible strategy in wound care- **Authors**: - Principal investigator and contact person: Dorota Chełminiak-Dudkiewicz, Nicolaus Copernicus University in Torun, e-mail: dorotachd@umk.pl.-Investigator: Miloslav Machacek, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Czech Republic, e-mail:machamil@faf.cuni.cz-Investigator: Jolanta Dlugaszewska, Poznan University of Medical Sciences, e-mail: jdlugasz@wp.pl-Investigator: Aleksander Smolarkiewicz-Wyczachowski, Nicolaus Copernicus University in Torun, e-mail: asmolarkiewicz@doktorant.umk.pl- **Date of data collection**: year 2025.- **Place of data collection**: Torun- **Keywords***: chitosan-kefiran films, dialdehyde kefiran, “Henola” hemp, plant extract, wound dressing materialsData language**: English- **Sources of funding**: National Science Center (NCN) competition: SONATA 18- UMO-2022/47/D/NZ7/01821.2. Overview of data and files- **File List**:Fig.1.tiff: Characterization of the native kefiran (Kef) and dialdehyde kefiran (DKef). The size distribution, SEM image, and 13C NMR spectra of the a) Kef and b) DKef; c) ATR-FTIR spectra of the Kef and DKef.Fig.2. tiff: Physicochemical properties of the obtained films. a) ATR-FTIR spectra, b) thermal analysis, c) SEM images, and d) AFM and profile images.Fig.3.tiff: Properties of the obtained films. a) swelling rate in different pH, b) biodegradation profile, c) antioxidant properties, d) antiinflammatory properties, and e), f) protein adsorption on the materials. *, #, $ indicate p &lt; 0.05 when compared to the corresponding CS/Kef and CS/Kef/WE, respectively. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extract HSA- human serum albumin FB-fibrinogen DPPH - 2,2-diphenyl-1-picrylhydrazyl radicalFig.4.tiff: Biological properties of the obtained films. a) antimicrobial properties against S. aureus, P. aeruginosa, and C. albicans, b) cytotoxicity (n = 3, expressed as mean (SD)), and c) hemolysis assay (n = 3, expressed as mean (SD)). Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extract PC- positive control NC- negative controlgraphical abstract.tiff: graphical abstractRaw data: swelling.tab: A swelling test was performed at pH 5.6 and pH 7.4. The samples were weighed, and the degree of swelling was calculated using a formula. The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extracthas adsorption.txt: The fluorescence method determined the adsorption of human serum albumin (HSA) on the materials. Fluorescence spectra were recorded at various time intervals at 25 C, ranging from 290 to 400 nm and 300 to 500 nm for HSA and Fb, respectively, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285 - 400 nm, scanning speed 100 nm/min, and Em/Ex bandwidth 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractAnti-inflammatory.txt: The raw data on the anti-inflammatory properties was determined by inhibiting the denaturation of bovine serum albumin (BSA) with the obtained materials. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractAntioxidant.txt: The raw data on the anti-inflammatory properties was determined by the DPPH scavenging test. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractfibrinogen adsorption.txt: The fluorescence method determined the adsorption of fibrinogen (FB) on the materials. Fluorescence spectra were recorded at various time intervals at 25 C, ranging from 290 to 400 nm and 300 to 500 nm for HSA and Fb, respectively, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285 - 400 nm, scanning speed 100 nm/min, and Em/Ex bandwidth 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extracthemolysis test.tab: The ex vivo hemolytic activity of CS/Kef, CS/Kef/WE, and CS/Kef/EE films was tested on human red blood cells from an anonymous donor (Approval of the Ethical Committee of the Faculty of Pharmacy in Hradec Králové, Charles University no. UKFaF/46675/2023). A study was performed by Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. It was measured using a Tecan Infinite 200M plate reader. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extract PC-positive control NC-negative controlbiodegradation.txt: The biodegradability of materials was determined by monitoring their weight changes for 14 days. The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractmicrobiological C albicans.txt: Study of antimicrobial properties against C. albicans. A study was performed by Dr. Jolanta Długaszewska from the Faculty of Pharmacy at the Medical University of Poznań. The analysis was carried out by culturing bacterial strains in a BHI medium in the presence of the material. The data was prepared using GraphPad Prism 10. List of abbreviations: BHI - Bacterial Growth Medium CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractMRC 24h.tab: Study of cytotoxicity properties against MRC cells after 24 hours of incubation. A study was conducted by Dr. Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. Fluorescence (λex = 560 nm, λem = 590 nm) was measured using the Tecan Infinite 200M plate reader (Tecan Group Ltd., Männedorf, Switzerland). The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractMicrobiologia P. aureginosa.txt: Study of antimicrobial properties against P. aureginosa bacteria. A study was performed by Dr. Jolanta Długaszewska from the Faculty of Pharmacy at the Medical University of Poznań. The analysis was carried out by culturing bacterial strains in a BHI medium in the presence of the material. The data was prepared using GraphPad Prism 10. List of abbreviations: BHI - Bacterial Growth Medium CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractmicrobiologia S.aureus.txt: Study of antimicrobial properties against S. auerus bacteria. A study was performed by Dr. Jolanta Długaszewska from the Faculty of Pharmacy at the Medical University of Poznań. The analysis was carried out by culturing bacterial strains in a BHI medium in the presence of the material. The data was prepared using GraphPad Prism 10. List of abbreviations: BHI - Bacterial Growth Medium CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extractMRC 74h.tab: Study of cytotoxicity properties against MRC cells after 72 hours of incubation. A study was conducted by Dr. Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. Fluorescence (λex = 560 nm, λem = 590 nm) was measured using the Tecan Infinite 200M plate reader (Tecan Group Ltd., Männedorf, Switzerland). The data was prepared using GraphPad Prism 10. Legend: CS/Kef - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran CS/Kef/WE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola water extract CS/Kef/EE - film based on chitosan and kefiran, crosslinked with dialdehyde kefiran and enriched with Henola ethanol extract- **File formats**: tiff, tab, txt3. sharing and access information- **Licenses/Restrictions**: CC BY - Creative Commons Attribution 4.0. - **Related Publications**: https://pubs.rsc.org/en/content/articlelanding/2025/ma/d5ma00029g