cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/qtw16-p9q80
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-12-19</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, place the tube on bench for ~20 min, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~20 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-47/3dimage_73119/</p>20151219_YWC206_10047_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-47/3dimage_73119/20151219_YWC206_10047_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20151219_YWC206_10047.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-47/rawdata/20151219_YWC206_10047.mrc"
        > <i class="download icon"></i> Download </a></p>

        20151219_YWC206_10047_SIRT_2K.rec~,  7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-47/file_16588/20151219_YWC206_10047_SIRT_2K.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sc2mj-4z324
title: Agro_AD1231

description: AD1231: pAD1361/A136; Carbenicillin 50;  virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.
description: <strong>Tilt Series Date:</strong> 2015-08-03</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 155.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow culture in AB medium (pH 7) at 30C. For induction, use 375 µl of a decent ON culture to inoculate 5 ml AB mes pH 5.8 medium containing 100 µM Acetosyringone and antibiotic (Cb 50). Grow at 20C for 18 hr. Concentrate cells 5 times by centrifuging. Freeze cells.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK III, MK IV was not working) settings: 100% humidity, 4µl sample, 5% BSA treated gold. Blot offset -5, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-39/3dimage_60053/</p>20150803_1231_10036_part121_12.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-39/3dimage_60053/20150803_1231_10036_part121_12.rec"
        > <i class="download icon"></i> Download </a></p>

        20150803_1231_10036.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-39/rawdata/20150803_1231_10036.mrc"
        > <i class="download icon"></i> Download </a></p>

        dga2015-08-03-39_slicer19255.png,  REZ .  -andrew, 815.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2015-08-03-39_slicer19255.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/611sg-74845
title: Helicobacter_Pylori_dFliO

description: These are H. pylori cells that don't have a complete FliP. In addition, FliO was knocked out in  .  these cells. The aim is to study the assembly of the flagellar motor and the core complex.
description: <strong>Tilt Series Date:</strong> 2019-09-21</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . flagella. We knocked out FliO protein of the flagellar motor to study the early assembly . . states of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1. 100% humidity. Cu grids. 10 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-5/rawdata/</p>Hpylori_dFliO3.mrc,  Tilt Series (Pixel Size 0.4494 nm), 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-5/rawdata/Hpylori_dFliO3.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_dFliO3_full.rec,  Reconstruction (Pixel Size 1.7976 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-5/3dimage_106676/Hpylori_dFliO3_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_dFliO3.mrc.mdoc,  28.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-5/rawdata/Hpylori_dFliO3.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wgqv6-cwg69
title: Helicobacter_Pylori_dFliO

description: These are H. pylori cells that don't have a complete FliP. In addition, FliO was knocked out in  .  these cells. The aim is to study the assembly of the flagellar motor and the core complex.
description: <strong>Tilt Series Date:</strong> 2019-09-21</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . flagella. We knocked out FliO protein of the flagellar motor to study the early assembly . . states of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1. 100% humidity. Cu grids. 10 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-28/rawdata/</p>Hpylori_dFliO20.mrc,  Tilt Series (Pixel Size 0.4494 nm), 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-28/rawdata/Hpylori_dFliO20.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_dFliO20_full.rec,  Reconstruction (Pixel Size 1.7976 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-28/3dimage_106725/Hpylori_dFliO20_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_dFliO20.mrc.mdoc,  28.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-28/rawdata/Hpylori_dFliO20.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ysc3n5
title: Occurrence Download

description: A dataset containing 610 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Diplectria",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 610 records from 24 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007149-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/xwwrf-7fw51
title: E. coli CusCFBA-CusC-K98H

description: Overexpressed CusCFBA efflux pump in E. coli C43 (DE3).Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-06-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Edward Yu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.</p>
  ycw2013-06-07-11_slicer7931.jpg: CusCFBA .  Escherichia coli ycw2013-06-07-11_slicer7932.jpg: CusCFBA? .  Escherichia coli ycw2013-06-07-11_slicer7933.jpg: filament .  Escherichia coli
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-11/rawdata/</p>CusCFBA_CusCK98H_20008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-11/rawdata/CusCFBA_CusCK98H_20008.mrc"
        > <i class="download icon"></i> Download </a></p>

        CusCFBA_CusCK98H_20008_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-11/3dimage_27243/CusCFBA_CusCK98H_20008_full.rec"
        > <i class="download icon"></i> Download </a></p>

        ycw2013-06-07-11_slicer19210.png,  732.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2013-06-07-11_slicer19210.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8mqfd-ptw83
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-03-31-23_slicer5939.jpg: Filaments ycw2014-03-31-23_slicer5940.jpg: Vesicles and granule
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-23/rawdata/</p>20140331_YWC097_10023.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-23/rawdata/20140331_YWC097_10023.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10023_part87_15.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-23/3dimage_40664/20140331_YWC097_10023_part87_15.rec"
        > <i class="download icon"></i> Download </a></p>

        ycw2014-03-31-23_slicer16451.png,  659.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/ycw2014-03-31-23_slicer16451.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8k6ch-f6c57
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-03-31-6_slicer9641.jpg: Helicobacter pylori .  Flat vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-6/rawdata/</p>20140331_YWC097_10006.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-6/rawdata/20140331_YWC097_10006.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10006.rec,  Reconstruction (Pixel Size 19.2 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-6/3dimage_40627/20140331_YWC097_10006.rec"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10006.rec,  Reconstruction (Pixel Size 19.2 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-6/3dimage_40636/20140331_YWC097_10006.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16995711
title: Raw data files for Roberge et al. PLoS Genetics

description: Zipped file containing raw data and images for the revised manuscript submitted to PLoS Genetics:

Twitching motility suppressors reveal a role for FimX in type IV pilus extension dynamics 

Nathan Roberge1, Nathan Yuen1, Hanjeong Harvey1, Taylor J. Ellison2, Courtney K. Ellison2, and Lori L. Burrows1*

1 Department of Biochemistry and Biomedical Sciences, and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada, L8S4K1             

2 Department of Microbiology, University of Georgia, Athens, GA, 30602
DOI: 10.5281/zenodo.16995712
title: Raw data files for Roberge et al. PLoS Genetics

description: Zipped file containing raw data and images for the revised manuscript submitted to PLoS Genetics:

Twitching motility suppressors reveal a role for FimX in type IV pilus extension dynamics 

Nathan Roberge1, Nathan Yuen1, Hanjeong Harvey1, Taylor J. Ellison2, Courtney K. Ellison2, and Lori L. Burrows1*

1 Department of Biochemistry and Biomedical Sciences, and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada, L8S4K1             

2 Department of Microbiology, University of Georgia, Athens, GA, 30602
DOI: 10.15468/dl.w2spnz
title: Occurrence Download

description: A dataset containing 38 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Vriesea carmeniae"
 ]
}

 The dataset includes 38 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007151-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/y4j7h-g8c39
title: Rhodopseudomonas palustrus

description: Fe-metabolizing bacterium that makes parallel membrane sheets for photosynthesis.  Membrane topology is the primary interest.Keywords: photosynthetic membrane sheets, parallel membranes
description: <strong>Tilt Series Date:</strong> 2007-06-22</p>
<strong>Data Taken By:</strong> Bill Tivol</p>
<strong>Species / Specimen:</strong> Rhodopseudomonas palustris</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-59.0°, 59.0°), step: 1 deg°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Diane Newman, PI, Lars Dietrich, Postdoc, Lars gave me the specimens</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in FEM medium +10% acetate at 30 deg C in bright light.</p>
<strong>Sample Preparation:</strong> quantifoil, 10 nm gold, 100% humidity</p>
  btf2007-06-22-6_slicer5168.jpg: Unknown btf2007-06-22-6_slicer5169.jpg: Unknown filament btf2007-06-22-6_slicer5192.jpg: Unknown btf2007-06-22-6_slicer5193.jpg: Unknown btf2007-06-22-6_slicer5197.jpg: Unknown Filament btf2007-06-22-6_slicer5199.jpg: Unknown Filament btf2007-06-22-6_slicer5203.jpg: Unknown filament btf2007-06-22-6_slicer5206.jpg: unknown filament btf2007-06-22-6_slicer5209.jpg: Unknown filaments btf2007-06-22-6_slicer5211.jpg: Unknown parallel filament btf2007-06-22-6_slicer5216.jpg: Unknown filament (parallel row) btf2007-06-22-6_slicer5217.jpg: Unknown filament (parallel row) btf2007-06-22-6_slicer5220.jpg: Unknown ring
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-06-22-6/rawdata/</p>Rp22Jun07_06.mrc,  Tilt Series (Pixel Size 0.625 nm), 990.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-06-22-6/rawdata/Rp22Jun07_06.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rp22Jun07_06_full.rec,  Reconstruction (Pixel Size 1.25 nm), 1.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/btf2007-06-22-6/3dimage_403/Rp22Jun07_06_full.rec"
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        Rp22Jun07_06.rec,  Reconstruction (Pixel Size 1.25 nm), 171.0 MB  
        
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