DOI: 10.5281/zenodo.13382997
title: Tailored Sticky Solutions: 3D-Printed Miconazole Buccal Films for Pediatric Oral Candidiasis - Underlying CT data

description: Underlying CT data of "Tailored Sticky Solutions: 3D-Printed Miconazole Buccal Films for Pediatric Oral Candidiasis"DOI: https://doi.org/10.1208/s12249-024-02908-5

by 

Konstantina Chachlioutaki, Anastasia Iordanopoulou, Orestis L. Katsamenis, Anestis Tsitsos, Savvas Koltsakidis, Pinelopi Anastasiadou, Dimitrios Andreadis, Vangelis Economou, Christos Ritzoulis, Dimitrios Tzetzis, Nikolaos Bouropoulos, Iakovos Xenikakis & Dimitrios Fatouros 

 



Authors and Affiliations





Department of Pharmacy Division of Pharmaceutical Technology, Aristotle University of Thessaloniki, Thessaloniki, Greece

Konstantina Chachlioutaki, Anastasia Iordanopoulou, Iakovos Xenikakis & Dimitrios Fatouros




Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Thessaloniki, Greece

Konstantina Chachlioutaki & Dimitrios Fatouros




μ-VIS X-Ray Imaging Centre, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, UK

Orestis L. Katsamenis




Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK

Orestis L. Katsamenis




Laboratory of Animal Food Products Hygiene - Veterinary Public Health, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

Anestis Tsitsos & Vangelis Economou




Digital Manufacturing and Materials Characterization Laboratory, School of Science and Technology, International Hellenic University, 14km Thessaloniki–N. Moudania, 57001, Thermi, Greece

Savvas Koltsakidis & Dimitrios Tzetzis




Department of Oral Medicine/Pathology, School of Dentistry, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

Pinelopi Anastasiadou & Dimitrios Andreadis




Department of Food Science and Technology, International Hellenic University, Sindos Campus, 57400, Thessaloniki, Greece

Christos Ritzoulis




Department of Materials Science, University of Patras, Rio, 26504, Patras, Greece

Nikolaos Bouropoulos




Foundation for Research and Technology Hellas, Institute of Chemical Engineering and High Temperature Chemical Processes, 26504, Patras, Greece

Nikolaos Bouropoulos
description: X-ray Microfocus Computed Tomography (μ-CT)

Static and time-lapsed X-ray microfocus Computed Tomography (μ-CT) was used to examine the specimen's microstructure in its dry state and to assess the dissolution mechanism via μ-CT-based videography.

The imaging took place at the University of Southampton’s μ-VIS X-ray Imaging Centre (muvis.org) / 3D X-ray Histology facility using a customized μCT scanner optimized for 3D X-ray histology (xrayhistology.org) (Katsamenis et al., 2023) based on Nikon’s XTH225ST system (Nikon Metrology, Castle Donington, UK).

The process involved:

First, the specimen was imaged in its dry state using parameters in Table SI. Next, the film was placed between polyurethane foam blocks, immersed in PBS at 25°C, and time-resolved imaging was performed at regular intervals with 10 consecutive scans, each lasting 15 minutes, totaling 150 minutes. Imaging occurred at the University of Southampton’s μ-VIS X-ray Imaging Centre using a customized Nikon XTH225ST system.

Analysis of the static session involved measuring total sample volume, crystalline phase volume (via threshold-based segmentation), and matrix volume. Ratios like crystal/matrix and crystal/volume were computed, and crystalline phase percentage was calculated.

For the time-resolved session, the scan volumes were analyzed in Fiji/ImageJ. A common longitudinal slice was selected for each volume, creating a time-stack for side-by-side time series rendering. Hydrated versus dry phase areas were quantified using threshold-based segmentation. Interface sizes formed during decomposition were measured using 2D local-thickness analysis, rendered as thickness maps. Dragonfly software was used for 3D visualization of the time series.

Acknowledgements

We acknowledge μ-VIS X-ray Imaging Centre (https://muvis.org), part of the National Facility for laboratory-based X-ray CT (nxct.ac.uk – EPSRC: EP/T02593X/1) at the University of Southampton for the provision of the μCTimaging, processing, and data management infrastructure, and NVIDIA for providing the NVIDIA RTX A6000 graphics card used for processing these datasets.
description: NOTE: 

If you cannot load any of the dragonfly session please try the following https://theobjectsassist.freshdesk.com/a/solutions/articles/48001079787