cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.23391327
title: Genome wide analyses reveal the population distinctiveness of the ‘<i>Nera del Mela</i>’ sheep

description: Italy has a long history of sheep breeding and counts several local populations that may represent a unique source of genetic diversity. Among these, Nera del Mela is a sheep genetic resource historically reared in Sicily but not officially recognised as a breed. In this study, we genotyped 36 individuals of Nera del Mela using the OvineSNP50K array, in order to estimate the genetic diversity and evaluate the population structure and relatedness with other Italian sheep breeds. Genetic diversity indices, and inbreeding estimated from runs of homozygosity (F<sub>ROH</sub>) revealed a moderate level of variability. Runs of homozygosity islands mapped candidate genes involved in the adaptation to local environment and immune response. Population genetic analyses using different approaches highlighted the hypothesis that this sheep possesses a defined genetic structure, especially if compared with other recognised breeds, despite the influence of other populations such as the Sicilian breeds. Overall, our findings represent a starting point for the possible official acknowledgement of this population, for the creation of a conservation plan, and thus for preserving this genomic heritage.HIGHLIGHTSNera de Mela sheep can be considered as a reservoir of genetic diversity.The results indicated a clear genetic differentiation from other populations and moderate level of genetic variability.Our findings represent a starting point for the creation of conservation plans. Nera de Mela sheep can be considered as a reservoir of genetic diversity. The results indicated a clear genetic differentiation from other populations and moderate level of genetic variability. Our findings represent a starting point for the creation of conservation plans.
DOI: 10.6084/m9.figshare.23391327.v1
title: Genome wide analyses reveal the population distinctiveness of the ‘<i>Nera del Mela</i>’ sheep

description: Italy has a long history of sheep breeding and counts several local populations that may represent a unique source of genetic diversity. Among these, Nera del Mela is a sheep genetic resource historically reared in Sicily but not officially recognised as a breed. In this study, we genotyped 36 individuals of Nera del Mela using the OvineSNP50K array, in order to estimate the genetic diversity and evaluate the population structure and relatedness with other Italian sheep breeds. Genetic diversity indices, and inbreeding estimated from runs of homozygosity (F<sub>ROH</sub>) revealed a moderate level of variability. Runs of homozygosity islands mapped candidate genes involved in the adaptation to local environment and immune response. Population genetic analyses using different approaches highlighted the hypothesis that this sheep possesses a defined genetic structure, especially if compared with other recognised breeds, despite the influence of other populations such as the Sicilian breeds. Overall, our findings represent a starting point for the possible official acknowledgement of this population, for the creation of a conservation plan, and thus for preserving this genomic heritage.HIGHLIGHTSNera de Mela sheep can be considered as a reservoir of genetic diversity.The results indicated a clear genetic differentiation from other populations and moderate level of genetic variability.Our findings represent a starting point for the creation of conservation plans. Nera de Mela sheep can be considered as a reservoir of genetic diversity. The results indicated a clear genetic differentiation from other populations and moderate level of genetic variability. Our findings represent a starting point for the creation of conservation plans.
DOI: 10.2908/hsw_mi05
title: Accidents at work by ISCO (NACE Rev. 2 activity A, C-N)
title: Accidents du travail par CITP (activité de la NACE Rev. 2 A, C-N)
title: Arbeitsunfälle nach ISCO (NACE Rev. 2 Tätigkeit A, C-N)
DOI: 10.2908/hsw_mi_emp01
title: Accidents at work of employees by sex and severity (NACE Rev. 2 activity Total)
title: Accidents du travail des salariés, par sexe et gravité (toutes activités inclues, au sens de la NACE Rev.2)
title: Arbeitsunfälle von Arbeitnehmern nach Geschlecht und Schweregrad (alle Wirtschaftszweige gemäß NACE Rev. 2)
DOI: 10.6084/m9.figshare.22100579
title: Antibiotic treatments and somatic cell count as phenotype to map QTL for mastitis susceptibility in Holstein cattle breed

description: Mastitis is one of the most significant diseases affecting dairy cattle profitability. A genome wide association study (GWAS) was performed using the selective genotyping approach to identify Quantitative Trait Loci (QTL) associated with mastitis susceptibility. According to the received antibiotic treatments in all their productive life, 52 lactating cows have been classified in resistant (no therapy and low SCC) and susceptible (more than one treatment and high SCC) to mastitis. Genotyping of animals was performed using the NEOGEN’s GGP Bovine 100K SNP chip and QTL were identified comparing the SNPs allelic frequencies between the two groups. 26 SNPs related to mastitis susceptibility were identified in 9 chromosomes. The use of treatment data, coupled with SCC from milk recording, improved cow’s classification accuracy in resistant and susceptible individuals. For this reason, the mandatory recording of treatments active from January 2022, could be a new source of information to improve the genetic selection for mastitis resistance.
DOI: 10.6084/m9.figshare.22100579.v1
title: Antibiotic treatments and somatic cell count as phenotype to map QTL for mastitis susceptibility in Holstein cattle breed

description: Mastitis is one of the most significant diseases affecting dairy cattle profitability. A genome wide association study (GWAS) was performed using the selective genotyping approach to identify Quantitative Trait Loci (QTL) associated with mastitis susceptibility. According to the received antibiotic treatments in all their productive life, 52 lactating cows have been classified in resistant (no therapy and low SCC) and susceptible (more than one treatment and high SCC) to mastitis. Genotyping of animals was performed using the NEOGEN’s GGP Bovine 100K SNP chip and QTL were identified comparing the SNPs allelic frequencies between the two groups. 26 SNPs related to mastitis susceptibility were identified in 9 chromosomes. The use of treatment data, coupled with SCC from milk recording, improved cow’s classification accuracy in resistant and susceptible individuals. For this reason, the mandatory recording of treatments active from January 2022, could be a new source of information to improve the genetic selection for mastitis resistance.
DOI: 10.6084/m9.figshare.23828521
title: Deviation of behavioural and productive parameters in dairy cows due to a lameness event: a synthesis of reviews

description: Lameness is a widespread multifactorial condition affecting the health and performance of dairy cows. Despite the growing support by precision farming technologies, farmers still lack reliable data-driven tools to early identify lame cows. This study used a synthesis of reviews to identify cow’s behavioural and productive parameters most related to lameness and estimate their deviation due to a lameness event. The methodological approach used reviews as starting point to identify the most pertinent studies with the intention of extracting and analysing data from these primary studies. The final dataset used information collected from 31 research papers, cited in 15 reviews, and involved more than 25,000 dairy cows. Five parameters were suitable for the meta-analysis: one about eating behaviour (eating time), three regarding activity and resting behaviour (lying bouts, lying bout duration and lying time) and milk yield. The meta-analysis revealed that all parameters had a significant deviation in cows affected by lameness. The calculation of the pooled means allowed to quantify a mean value for the deviation imposed by a severe lameness event from the value recorded on nonlame cows. Compared to a nonlame animal, a lame cow had a significant negative deviation for eating time (−39 min/day), number of lying bouts (−0.5/day), and milk yield (−3 kg/day). Lame cows had positive deviations for lying bout duration (+12 min/bout) and daily lying time (+42 min/day). The individual or combined use of these mean deviation values as alarm reference thresholds could improve the accuracy of the current automated lameness detection systems. Feeding and resting habits, and milk yield had a significant deviation in lame cows.Lame cows decreased their time spent eating, lying bouts frequency, and milk yield.Lame cows had prolonged lying bout duration and daily lying time. Feeding and resting habits, and milk yield had a significant deviation in lame cows. Lame cows decreased their time spent eating, lying bouts frequency, and milk yield. Lame cows had prolonged lying bout duration and daily lying time.
DOI: 10.6084/m9.figshare.23828521.v2
title: Deviation of behavioural and productive parameters in dairy cows due to a lameness event: a synthesis of reviews

description: Lameness is a widespread multifactorial condition affecting the health and performance of dairy cows. Despite the growing support by precision farming technologies, farmers still lack reliable data-driven tools to early identify lame cows. This study used a synthesis of reviews to identify cow’s behavioural and productive parameters most related to lameness and estimate their deviation due to a lameness event. The methodological approach used reviews as starting point to identify the most pertinent studies with the intention of extracting and analysing data from these primary studies. The final dataset used information collected from 31 research papers, cited in 15 reviews, and involved more than 25,000 dairy cows. Five parameters were suitable for the meta-analysis: one about eating behaviour (eating time), three regarding activity and resting behaviour (lying bouts, lying bout duration and lying time) and milk yield. The meta-analysis revealed that all parameters had a significant deviation in cows affected by lameness. The calculation of the pooled means allowed to quantify a mean value for the deviation imposed by a severe lameness event from the value recorded on nonlame cows. Compared to a nonlame animal, a lame cow had a significant negative deviation for eating time (−39 min/day), number of lying bouts (−0.5/day), and milk yield (−3 kg/day). Lame cows had positive deviations for lying bout duration (+12 min/bout) and daily lying time (+42 min/day). The individual or combined use of these mean deviation values as alarm reference thresholds could improve the accuracy of the current automated lameness detection systems. Feeding and resting habits, and milk yield had a significant deviation in lame cows.Lame cows decreased their time spent eating, lying bouts frequency, and milk yield.Lame cows had prolonged lying bout duration and daily lying time. Feeding and resting habits, and milk yield had a significant deviation in lame cows. Lame cows decreased their time spent eating, lying bouts frequency, and milk yield. Lame cows had prolonged lying bout duration and daily lying time.
DOI: 10.6084/m9.figshare.24440690
title: Analysis of fatty acid composition and miRNA profile in adipose tissue after application of <i>Allium mongolicum</i> Regel extract in diet of sheep

description: Extensive regulation of microRNAs (miRNAs) that belong to a type of endogenous small noncoding RNAs on gene expression in sheep has been recognised. Meanwhile, utilisation of water extract of <i>Allium mongolicum</i> Regel (WEA) in diet of sheep has been proved to regulate fatty acid composition and flavour in mutton. However, the regulatory mechanism remains unclear. We conducted miRNA and mRNA sequencing in this study. Briefly, 30 male small-tailed Han sheep (4.5 months old) were randomly assigned to two groups. A basal diet with 3.4 g·sheep<sup>−1</sup>·d<sup>−1</sup> WEA supplementation (WEA group) or with no WEA supplementation (CON group) was fed to sheep, respectively. Subcutaneous and visceral adipose tissue samples were collected during slaughter after 75-day feeding experiment. We obtained 30 differentially expressed miRNAs (DE-miRNAs) after application of WEA in diet of sheep in subcutaneous adipose tissue. Likewise, 15 DE-miRNAs in visceral adipose tissue were identified. A total of 746 differentially expressed mRNAs (DE-mRNAs) with 90 lipid-related DE-mRNAs in subcutaneous adipose tissue and 569 DE-mRNAs with 73 lipid-related DE-mRNAs in visceral adipose tissue were identified after WEA supplementation. The intersection genes from predicted target genes of DE-miRNAs by databases and actual DE-mRNAs by mRNA sequencing were enriched in several lipid-related pathways both in subcutaneous adipose and in visceral adipose tissue. We constructed differential miRNAs-mRNAs networks by Cytoscape software. Our results will help to explain regulatory mechanism of fatty acid composition in mutton by dietary WEA and will provide a theoretical basis for rational use of the plant extract in diet of sheep.HIGHLIGHTSUtilisation of <i>Allium mongolicum</i> Regel water extract led to alterations of endogenous miRNA and gene expression profiles in subcutaneous and visceral adipose tissue of sheep.<i>Allium mongolicum</i> Regel water extract improved nutritional value and flavour of mutton by changing fatty acid composition in adipose tissue.Establishment of miRNA-mRNA regulatory network with regard to manipulation of fatty acid composition in mutton by <i>Allium mongolicum</i> Regel water extract, and provided a theoretical basis for rational utilisation of this plant extract in diet of sheep. Utilisation of <i>Allium mongolicum</i> Regel water extract led to alterations of endogenous miRNA and gene expression profiles in subcutaneous and visceral adipose tissue of sheep. <i>Allium mongolicum</i> Regel water extract improved nutritional value and flavour of mutton by changing fatty acid composition in adipose tissue. Establishment of miRNA-mRNA regulatory network with regard to manipulation of fatty acid composition in mutton by <i>Allium mongolicum</i> Regel water extract, and provided a theoretical basis for rational utilisation of this plant extract in diet of sheep.
DOI: 10.6084/m9.figshare.24440690.v1
title: Analysis of fatty acid composition and miRNA profile in adipose tissue after application of <i>Allium mongolicum</i> Regel extract in diet of sheep

description: Extensive regulation of microRNAs (miRNAs) that belong to a type of endogenous small noncoding RNAs on gene expression in sheep has been recognised. Meanwhile, utilisation of water extract of <i>Allium mongolicum</i> Regel (WEA) in diet of sheep has been proved to regulate fatty acid composition and flavour in mutton. However, the regulatory mechanism remains unclear. We conducted miRNA and mRNA sequencing in this study. Briefly, 30 male small-tailed Han sheep (4.5 months old) were randomly assigned to two groups. A basal diet with 3.4 g·sheep<sup>−1</sup>·d<sup>−1</sup> WEA supplementation (WEA group) or with no WEA supplementation (CON group) was fed to sheep, respectively. Subcutaneous and visceral adipose tissue samples were collected during slaughter after 75-day feeding experiment. We obtained 30 differentially expressed miRNAs (DE-miRNAs) after application of WEA in diet of sheep in subcutaneous adipose tissue. Likewise, 15 DE-miRNAs in visceral adipose tissue were identified. A total of 746 differentially expressed mRNAs (DE-mRNAs) with 90 lipid-related DE-mRNAs in subcutaneous adipose tissue and 569 DE-mRNAs with 73 lipid-related DE-mRNAs in visceral adipose tissue were identified after WEA supplementation. The intersection genes from predicted target genes of DE-miRNAs by databases and actual DE-mRNAs by mRNA sequencing were enriched in several lipid-related pathways both in subcutaneous adipose and in visceral adipose tissue. We constructed differential miRNAs-mRNAs networks by Cytoscape software. Our results will help to explain regulatory mechanism of fatty acid composition in mutton by dietary WEA and will provide a theoretical basis for rational use of the plant extract in diet of sheep.HIGHLIGHTSUtilisation of <i>Allium mongolicum</i> Regel water extract led to alterations of endogenous miRNA and gene expression profiles in subcutaneous and visceral adipose tissue of sheep.<i>Allium mongolicum</i> Regel water extract improved nutritional value and flavour of mutton by changing fatty acid composition in adipose tissue.Establishment of miRNA-mRNA regulatory network with regard to manipulation of fatty acid composition in mutton by <i>Allium mongolicum</i> Regel water extract, and provided a theoretical basis for rational utilisation of this plant extract in diet of sheep. Utilisation of <i>Allium mongolicum</i> Regel water extract led to alterations of endogenous miRNA and gene expression profiles in subcutaneous and visceral adipose tissue of sheep. <i>Allium mongolicum</i> Regel water extract improved nutritional value and flavour of mutton by changing fatty acid composition in adipose tissue. Establishment of miRNA-mRNA regulatory network with regard to manipulation of fatty acid composition in mutton by <i>Allium mongolicum</i> Regel water extract, and provided a theoretical basis for rational utilisation of this plant extract in diet of sheep.
DOI: 10.57701/suf.vvl.2007.1-0
title: Vollendete Versichertenleben 2007
title: SUF
title: VVL

description: Scientific Use File Vollendete Versichertenleben (SUF.VVL):Grundlage des Verlaufsdatensatzes SUF.VVL ist die Rentenzugangsstatistik (§ 6 RSVwV, SK90) verknüpft mit Daten im Format der Versichertenkontenstichprobe (§ 1 RSVwV Abs. 2, SK79). Die Grundgesamtheit bildet der Versichertenrentenzugang eingeschränkt auf Alters- und Erwerbsminderungsrenten sowie definierte Meldegründe (insbesondere erstmaliger Rentenbezug). Die Größe der Zufallsstichprobe beträgt 25% Prozent.Berichtet wird neben demographischen Angaben über wichtige rentenrechtliche Sachverhalte wie beispielsweise Rentenhöhe, Komponenten des Rentenzahlbetrages, Entgeltpunkte sowie angerechnete versicherungsrechtliche Zeiten. Die Verlaufsdaten beschreiben die soziale Erwerbssituation während des Versichertenlebens, aber auch Sachverhalte wie beispielsweise Krankheit, Arbeitslosigkeit, Kindererziehung und Entgelt monatsgenau. Die monatlichen Angaben sind dabei lebenszeitbezogen (Januar des 14. bis Dezember des 65. Lebensjahres) als Querschnitt organisiert.
DOI: 10.6084/m9.figshare.23931300
title: Genomic approach to manage genetic variability in dairy farms

description: In this study we investigated the genetic variability, the inbreeding and allele frequencies of monogenic traits in seven herds of Holstein breed and provided insight to farmers on the value of genomic management of reproduction in their herds. A total of 3,953 Holstein cows were sampled and genotyped with the Neogen GGP Bovine 100K SNP chip within the activities of the Regione Lombardia funded GO-PEI project ‘GENOmic tool for the management of reproduction in dairy cattle and for the control of inbreeding – GENORIP’. Principal component analysis was applied for analysing the genetic variability within and among farms using the SVS software of Golden Helix. Run of Homozygosity (ROH) and the genomic inbreeding were obtained with the detectRUNS package of the R software. Genotype frequencies for mendelian disease, fertility and production traits were also obtained. A total of 458,267 ROH were identified and ROH were distributed on all autosomes with an average length of 2,703,811 bp covering 12.7% of the genome. Several genomic regions appear under selection, while a specific region on BTA4 was identified in one herd, harbouring genes mainly related to the specific selection strategy of the farmer. The F<sub>ROH</sub> values obtained considering ROH greater than 16 Mb, varied from 0.004 to 0.325, with the highest F<sub>ROH</sub> average value of 0.136. Among mendelian heritable diseases, the Haplotype Cholesterol Deficiency was the one with the largest proportion of carrier animals, i.e. 5.6%. A herd-tailored process to assist farmers in genomic management of reproduction was released. The ROH distribution within herd, together with the genotype frequencies for disease, fertility and production mendelian traits, suggest that similar directional selection is occurring across herds. This study released to each farmer the genomic make-up of their herd used jointly with the gEBV estimated by their national breeders’ association (ANAFIBJ) for herd reproductive management. All females of 7 herds have been genotyped with the GGP 100K SNP chip. Genomic information on all females can be used by farmers in the process to manage reproduction, selection and genetic herd variability. The availability of genomic information on the whole herd allowed to release to each farmer the genomic make-up of their herd. The ROH distribution together with the genotype frequencies for disease, fertility and production mendelian traits, made it possible to identify genomic regions under selection according to farmer strategies.
DOI: 10.6084/m9.figshare.23931300.v1
title: Genomic approach to manage genetic variability in dairy farms

description: In this study we investigated the genetic variability, the inbreeding and allele frequencies of monogenic traits in seven herds of Holstein breed and provided insight to farmers on the value of genomic management of reproduction in their herds. A total of 3,953 Holstein cows were sampled and genotyped with the Neogen GGP Bovine 100K SNP chip within the activities of the Regione Lombardia funded GO-PEI project ‘GENOmic tool for the management of reproduction in dairy cattle and for the control of inbreeding – GENORIP’. Principal component analysis was applied for analysing the genetic variability within and among farms using the SVS software of Golden Helix. Run of Homozygosity (ROH) and the genomic inbreeding were obtained with the detectRUNS package of the R software. Genotype frequencies for mendelian disease, fertility and production traits were also obtained. A total of 458,267 ROH were identified and ROH were distributed on all autosomes with an average length of 2,703,811 bp covering 12.7% of the genome. Several genomic regions appear under selection, while a specific region on BTA4 was identified in one herd, harbouring genes mainly related to the specific selection strategy of the farmer. The F<sub>ROH</sub> values obtained considering ROH greater than 16 Mb, varied from 0.004 to 0.325, with the highest F<sub>ROH</sub> average value of 0.136. Among mendelian heritable diseases, the Haplotype Cholesterol Deficiency was the one with the largest proportion of carrier animals, i.e. 5.6%. A herd-tailored process to assist farmers in genomic management of reproduction was released. The ROH distribution within herd, together with the genotype frequencies for disease, fertility and production mendelian traits, suggest that similar directional selection is occurring across herds. This study released to each farmer the genomic make-up of their herd used jointly with the gEBV estimated by their national breeders’ association (ANAFIBJ) for herd reproductive management. All females of 7 herds have been genotyped with the GGP 100K SNP chip. Genomic information on all females can be used by farmers in the process to manage reproduction, selection and genetic herd variability. The availability of genomic information on the whole herd allowed to release to each farmer the genomic make-up of their herd. The ROH distribution together with the genotype frequencies for disease, fertility and production mendelian traits, made it possible to identify genomic regions under selection according to farmer strategies.