cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/ujjjq2
title: INBO collaborator Occurrences of fungi recorded in waarnemingen.be in Flanders, Belgium

description: This dataset comprises fungi occurrence records sourced from the Waarnemingen database, with a focus on data tagged to the Research Institute for Nature and Forest (INBO). The majority of observations stem from INBO-led fieldwork, systematically recorded using waarnemingen.be and the ObsMapp mobile application. In addition, the dataset includes a selection of ad hoc observations contributed by INBO users within the platform.

All records were extracted from the central Waarnemingen database, managed by Natuurpunt and part of the observation.org network. Each entry includes species identification, observation date, location details, observer information, and method of recording. This curated dataset provides valuable insights into plant biodiversity across Flanders and supports research, monitoring, and conservation initiatives.

INBO tagged occurrences of non-native fungi, plants and animals are already published in https://www.gbif.org/dataset/9a0b66df-7535-4f28-9f4e-5bc11b8b096c and https://www.gbif.org/dataset/7f5e4129-0717-428e-876a-464fbd5d9a47 and are kept out of this dataset.

To allow anyone to use this dataset, we have released the data to the public domain under a Creative Commons Zero waiver. We would appreciate however, if you read and follow these norms for data use and provide a link to the original dataset whenever possible.
DOI: 10.5281/zenodo.16878242
title: Morphometric data from: Evolving to invade: Using geometric morphometrics to assess wing shape variation in the Antarctic non-native fly Trichocera maculipennis

description: This dataset contains raw morphometric measurements obtained from 294 wings of Trichocera maculipennis collected during Antarctic summer campaigns (2017/18 to 2019/20 and 2022/23).
description: Globalization has increased human movement, transforming ecosystems and introducing non-native species that threaten biodiversity, particularly in Antarctica. Due to its harsh climatic conditions, Antarctica has largely remained protected from biological invasions. However, rising human activity and environmental changes are facilitating the introduction of non-native species. The risks posed by species like Trichocera maculipennis are significant, as they can adapt and survive in new environments. This highlights the importance of understanding the adaptive factors that contribute to their success in order to prevent future invasions and protect the continent's unique ecosystem. The aim of this study was to quantify the wing morphological variation of T. maculipennis individuals associated with different Antarctic bases, analyzing this variation within a spatial and temporal context using morphometric tools. Samples were collected during Antarctic summer campaigns (2017/18 to 2019/20 and 2022/23) at the following research stations on King George Island: Artigas (Uruguay), Bellingshausen (Russia), Profesor Julio Escudero (Chile), and King Sejong (Korea). Morphometric analyses revealed changes in wing shape among study sites, with King Sejong individuals showing the most significant differences. Additionally, a temporal shift in wing size was observed, with specimens from 2017/18 and 2018/19 having larger average wings compared to those from 2019/20 and 2021/22. These results emphasize the importance of understanding the factors contributing to the success of non-native species to prevent future invasions and protect the unique ecosystem of Antarctica.
DOI: 10.5281/zenodo.16878241
title: Morphometric data from: Evolving to invade: Using geometric morphometrics to assess wing shape variation in the Antarctic non-native fly Trichocera maculipennis

description: This dataset contains raw morphometric measurements obtained from 294 wings of Trichocera maculipennis collected during Antarctic summer campaigns (2017/18 to 2019/20 and 2022/23).
description: Globalization has increased human movement, transforming ecosystems and introducing non-native species that threaten biodiversity, particularly in Antarctica. Due to its harsh climatic conditions, Antarctica has largely remained protected from biological invasions. However, rising human activity and environmental changes are facilitating the introduction of non-native species. The risks posed by species like Trichocera maculipennis are significant, as they can adapt and survive in new environments. This highlights the importance of understanding the adaptive factors that contribute to their success in order to prevent future invasions and protect the continent's unique ecosystem. The aim of this study was to quantify the wing morphological variation of T. maculipennis individuals associated with different Antarctic bases, analyzing this variation within a spatial and temporal context using morphometric tools. Samples were collected during Antarctic summer campaigns (2017/18 to 2019/20 and 2022/23) at the following research stations on King George Island: Artigas (Uruguay), Bellingshausen (Russia), Profesor Julio Escudero (Chile), and King Sejong (Korea). Morphometric analyses revealed changes in wing shape among study sites, with King Sejong individuals showing the most significant differences. Additionally, a temporal shift in wing size was observed, with specimens from 2017/18 and 2018/19 having larger average wings compared to those from 2019/20 and 2021/22. These results emphasize the importance of understanding the factors contributing to the success of non-native species to prevent future invasions and protect the unique ecosystem of Antarctica.
DOI: 10.6084/m9.figshare.30620793
title: Red meat intake and its influences on inflammation and immune function biomarkers in human adults: a systematic review and meta-analysis of randomized controlled trials and observational studies

description: Red meat intake (RMI) is implicated in promoting inflammation and compromising immune function. We conducted a systematic review and meta-analysis assessing how RMI influences inflammation and immune function biomarkers in adults with any health status. Twenty-two randomized controlled trials (RCTs; 1152 adults) and 10 observational studies (OBS; 438,925 adults) were included from literature published through May 2024 using PubMed, Scopus, CINAHL, and Cochrane databases. Sufficient data were obtained for meta-analyses of blood C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), leptin, and adiponectin. Overall, greater total RMI led to higher CRP across 18 RCTs (weighted mean difference 0.23, 95% CI 0.08–0.39). Subgroup analyses indicated similar findings for participants diagnosed with, but not without, cardiometabolic diseases; unspecified or mixed RMI, but not unprocessed RMI; studies without, but not with, energy restriction; and RMI ≥, but not &lt;, 0.5 servings/day (3.5 servings/week). Limited RCTs suggest total RMI did not influence IL-6, TNF-α, leptin, or adiponectin. Among 10 OBS, no associations were shown between RMI and these biomarkers. Conservatively, these results are consistent with recommendations for people who choose to consume red meat to limit or avoid consuming processed red meat, especially among individuals with cardiometabolic diseases. <b>Registry and registry number for systematic reviews or meta-analyses:</b> PROSPERO CRD42021256264
DOI: 10.6084/m9.figshare.30620793.v1
title: Red meat intake and its influences on inflammation and immune function biomarkers in human adults: a systematic review and meta-analysis of randomized controlled trials and observational studies

description: Red meat intake (RMI) is implicated in promoting inflammation and compromising immune function. We conducted a systematic review and meta-analysis assessing how RMI influences inflammation and immune function biomarkers in adults with any health status. Twenty-two randomized controlled trials (RCTs; 1152 adults) and 10 observational studies (OBS; 438,925 adults) were included from literature published through May 2024 using PubMed, Scopus, CINAHL, and Cochrane databases. Sufficient data were obtained for meta-analyses of blood C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), leptin, and adiponectin. Overall, greater total RMI led to higher CRP across 18 RCTs (weighted mean difference 0.23, 95% CI 0.08–0.39). Subgroup analyses indicated similar findings for participants diagnosed with, but not without, cardiometabolic diseases; unspecified or mixed RMI, but not unprocessed RMI; studies without, but not with, energy restriction; and RMI ≥, but not &lt;, 0.5 servings/day (3.5 servings/week). Limited RCTs suggest total RMI did not influence IL-6, TNF-α, leptin, or adiponectin. Among 10 OBS, no associations were shown between RMI and these biomarkers. Conservatively, these results are consistent with recommendations for people who choose to consume red meat to limit or avoid consuming processed red meat, especially among individuals with cardiometabolic diseases. <b>Registry and registry number for systematic reviews or meta-analyses:</b> PROSPERO CRD42021256264
DOI: 10.6084/m9.figshare.30620968
title: Beyond bad: early ERP correlates of written swearword processing

description: The aim of the study was to analyse brain responses to offensive words and compare them with non-vulgar negative words. We hypothesised differences in ERP amplitudes within the first 200 ms after the word’s onset and late modulation related to the processing of vulgar words. Twenty-five volunteers viewed briefly presented (500 ms) sets (each <i>n</i> = 20) of vulgar, negative, and neutral adjectives. Four time windows were analysed, corresponding to the N1 (80–120 ms), the P150 (130–180 ms), the N400 (350–450 ms), and the late positive potential (LPP, 500–700 ms) components of the ERP. There were no differences noted on the earliest component. There was a significant result on the P150 with the highest amplitudes evoked to negative adjectives and the lowest amplitudes evoked to the vulgar adjectives. The effect indicates less demanding attentional engagement for offensive language during the first lexico-semantic analysis stage. There was also LPP modulation with the highest amplitudes evoked in response to the vulgar words. Swearwords seem to be a special category marked early and late in visual processing, based not on valence or arousal attributes, but rather on social taboo defiance, which does not necessitate heightened levels of attentional capture, but engages more elaborate semantic re-analysis.
DOI: 10.6084/m9.figshare.30620968.v1
title: Beyond bad: early ERP correlates of written swearword processing

description: The aim of the study was to analyse brain responses to offensive words and compare them with non-vulgar negative words. We hypothesised differences in ERP amplitudes within the first 200 ms after the word’s onset and late modulation related to the processing of vulgar words. Twenty-five volunteers viewed briefly presented (500 ms) sets (each <i>n</i> = 20) of vulgar, negative, and neutral adjectives. Four time windows were analysed, corresponding to the N1 (80–120 ms), the P150 (130–180 ms), the N400 (350–450 ms), and the late positive potential (LPP, 500–700 ms) components of the ERP. There were no differences noted on the earliest component. There was a significant result on the P150 with the highest amplitudes evoked to negative adjectives and the lowest amplitudes evoked to the vulgar adjectives. The effect indicates less demanding attentional engagement for offensive language during the first lexico-semantic analysis stage. There was also LPP modulation with the highest amplitudes evoked in response to the vulgar words. Swearwords seem to be a special category marked early and late in visual processing, based not on valence or arousal attributes, but rather on social taboo defiance, which does not necessitate heightened levels of attentional capture, but engages more elaborate semantic re-analysis.
DOI: 10.6084/m9.figshare.30620974
title: Unbinding pathways and energetics of papaya-derived flavonoids as dengue MTase inhibitors via SMD-US and MMGBSA methods

description: Dengue virus methyltransferase (DENV MTase) facilitates the methylation of the viral RNA cap and continues to be a confirmed target for antiviral development. This study examined papaya-derived flavonoids as potential MTase inhibitors through an extensive computational workflow that integrated molecular docking, molecular dynamics (MD) simulations, MM/GBSA binding energy, and steered molecular dynamics–umbrella sampling (SMD–US) to assess unbinding energetics. Neohesperidin, Quercetin, and Myricetin exhibited the highest binding affinities for DENV MTase among the analysed flavonoids, with binding free energies (ΔG<sub>US</sub>) of −15.31, −14.67, and −13.69 kcal/mol, respectively from umbrella sampling. Potential mean force (PMF) profiles derived from umbrella sampling offer insights about the unbinding mechanisms and thermodynamic feasibility of these naturally occurring inhibitors. The results of MMGBSA binding energy and the identification of key amino acids at the active site, namely ASP131, LYS105, and ILE147, in the context of MTase-flavonoid binding through non-covalent interaction analysis further corroborated these conclusions. To evaluate Carica papaya-derived flavonoids as MTase inhibitors, this work is the first to combine SMD-US and MMGBSA analyses. The findings substantiate the promise of papaya-associated flavonoids as scaffolds for dengue MTase inhibition.
DOI: 10.6084/m9.figshare.30620974.v1
title: Unbinding pathways and energetics of papaya-derived flavonoids as dengue MTase inhibitors via SMD-US and MMGBSA methods

description: Dengue virus methyltransferase (DENV MTase) facilitates the methylation of the viral RNA cap and continues to be a confirmed target for antiviral development. This study examined papaya-derived flavonoids as potential MTase inhibitors through an extensive computational workflow that integrated molecular docking, molecular dynamics (MD) simulations, MM/GBSA binding energy, and steered molecular dynamics–umbrella sampling (SMD–US) to assess unbinding energetics. Neohesperidin, Quercetin, and Myricetin exhibited the highest binding affinities for DENV MTase among the analysed flavonoids, with binding free energies (ΔG<sub>US</sub>) of −15.31, −14.67, and −13.69 kcal/mol, respectively from umbrella sampling. Potential mean force (PMF) profiles derived from umbrella sampling offer insights about the unbinding mechanisms and thermodynamic feasibility of these naturally occurring inhibitors. The results of MMGBSA binding energy and the identification of key amino acids at the active site, namely ASP131, LYS105, and ILE147, in the context of MTase-flavonoid binding through non-covalent interaction analysis further corroborated these conclusions. To evaluate Carica papaya-derived flavonoids as MTase inhibitors, this work is the first to combine SMD-US and MMGBSA analyses. The findings substantiate the promise of papaya-associated flavonoids as scaffolds for dengue MTase inhibition.
DOI: 10.6084/m9.figshare.29099280
title: Development and validation of a detection method of alendronate in human plasma by high-performance liquid chromatography/tandem mass spectrometry (HPLC–MS/MS)

description: The aim of this study is to develop a method for detecting alendronate in human plasma. Due to its special chemical properties, alendronate is almost unretained on reverse-phase chromatography columns, and cannot be detected by UV or fluorescence detectors. Consequently, quantitative analysis and related research of alendronate in biological samples are extremely challenging. We developed a simple, sensitive and stable method for detecting alendronate in human plasma. In this method, plasma samples was treated with 10% trifluoroacetic acid to remove proteins and derivatized with trimethylsilyl diazomethane, then these samples were injected into the system for analysis using a PFP chromatographic column. The total run time was 10 min, and retention times of alendronate and Pamidronate disodium were 4.79 min and 4.00 min, respectively. The intra-batch and inter-batch accuracy values are between 90.88% and 104.50%, with CV% between 0.45% and 7.15%. And the <i>R</i><sup>2</sup> value of the calibration curve was greater than 0.99 in the range of 2.015–793.333 ng/mL. The recovery of quality control samples at different concentrations were greater than 100%. This method had been validated to detect the concentration of alendronate in human plasma and can provide technical reference for clinical researchers to conduct pharmacokinetic studies of alendronate.
DOI: 10.6084/m9.figshare.29099280.v1
title: Development and validation of a detection method of alendronate in human plasma by high-performance liquid chromatography/tandem mass spectrometry (HPLC–MS/MS)

description: The aim of this study is to develop a method for detecting alendronate in human plasma. Due to its special chemical properties, alendronate is almost unretained on reverse-phase chromatography columns, and cannot be detected by UV or fluorescence detectors. Consequently, quantitative analysis and related research of alendronate in biological samples are extremely challenging. We developed a simple, sensitive and stable method for detecting alendronate in human plasma. In this method, plasma samples was treated with 10% trifluoroacetic acid to remove proteins and derivatized with trimethylsilyl diazomethane, then these samples were injected into the system for analysis using a PFP chromatographic column. The total run time was 10 min, and retention times of alendronate and Pamidronate disodium were 4.79 min and 4.00 min, respectively. The intra-batch and inter-batch accuracy values are between 90.88% and 104.50%, with CV% between 0.45% and 7.15%. And the <i>R</i><sup>2</sup> value of the calibration curve was greater than 0.99 in the range of 2.015–793.333 ng/mL. The recovery of quality control samples at different concentrations were greater than 100%. This method had been validated to detect the concentration of alendronate in human plasma and can provide technical reference for clinical researchers to conduct pharmacokinetic studies of alendronate.
DOI: 10.6084/m9.figshare.29262734
title: Determination of polycyclic aromatic sulfur hydrocarbons (PASH) in petroleum asphalt fractions and their fumes by high-performance liquid chromatography with pulsed amperometric detection

description: Asphalt binders are primarily composed of aromatic hydrocarbons, but they also contain sulfur compounds that play a critical role in the chemical aging of the matrix. This study presents the development and validation of a high-performance liquid chromatography method with pulsed amperometric detection (HPLC-PAD) for the determination of polycyclic aromatic sulfur heterocycles (PASHs) in asphalt fractions and emissions. A mixture of 32 PASH standards was grouped into 20 analytically distinguishable peaks under optimized conditions. The method enabled quantification of PASHs at sub-ppm levels in both volatile (0.08–5.37 mg kg<sup>−1</sup>) and nonvolatile (0.02–0.38 mg kg<sup>−1</sup>) fractions. The presence of specific PASHs, including methyl- and dimethyl-substituted thiophenes, was also qualitatively confirmed by APPI-MS/MS analysis. These findings demonstrate the potential of the HPLC-PAD method as a reliable and cost-effective approach for screening sulfur-containing polyaromatic compounds in complex asphalt matrices.
DOI: 10.6084/m9.figshare.29262734.v1
title: Determination of polycyclic aromatic sulfur hydrocarbons (PASH) in petroleum asphalt fractions and their fumes by high-performance liquid chromatography with pulsed amperometric detection

description: Asphalt binders are primarily composed of aromatic hydrocarbons, but they also contain sulfur compounds that play a critical role in the chemical aging of the matrix. This study presents the development and validation of a high-performance liquid chromatography method with pulsed amperometric detection (HPLC-PAD) for the determination of polycyclic aromatic sulfur heterocycles (PASHs) in asphalt fractions and emissions. A mixture of 32 PASH standards was grouped into 20 analytically distinguishable peaks under optimized conditions. The method enabled quantification of PASHs at sub-ppm levels in both volatile (0.08–5.37 mg kg<sup>−1</sup>) and nonvolatile (0.02–0.38 mg kg<sup>−1</sup>) fractions. The presence of specific PASHs, including methyl- and dimethyl-substituted thiophenes, was also qualitatively confirmed by APPI-MS/MS analysis. These findings demonstrate the potential of the HPLC-PAD method as a reliable and cost-effective approach for screening sulfur-containing polyaromatic compounds in complex asphalt matrices.
DOI: 10.6084/m9.figshare.28615926
title: Immunotherapeutic effects of TCL-E5 and TCL-E5-pulsed DCs: two novel HPV therapeutic vaccine candidates

description: This study investigated the potential of HPV16 E5 oncoprotein-modified tumor cell lysate (TCL-E5) and dendritic cells (DCs) pulsed with TCL-E5 (TCL-E5-pulsed DCs) to enhance antitumor immunity in a murine model. For generation of TCL-E5, TC1 tumor cells were transduced with lentiviral particles harboring E5 protein. Moreover, the cell supernatants were prepared from DCs pulsed with TCL-E5. Their immunological responses and antitumor effects were investigated in a mouse model. The TCL-E5-pulsed DCs regimen could direct immunity toward Th1 and CTL responses, leading to tumor volume reduction and high percentage of tumor-free mice. The TCL-pulsed DCs regimen could not induce significant antitumor effects compared to TCL-E5-pulsed-DCs regimen indicating main role of E5 in vaccine development.
DOI: 10.6084/m9.figshare.28615926.v1
title: Immunotherapeutic effects of TCL-E5 and TCL-E5-pulsed DCs: two novel HPV therapeutic vaccine candidates

description: This study investigated the potential of HPV16 E5 oncoprotein-modified tumor cell lysate (TCL-E5) and dendritic cells (DCs) pulsed with TCL-E5 (TCL-E5-pulsed DCs) to enhance antitumor immunity in a murine model. For generation of TCL-E5, TC1 tumor cells were transduced with lentiviral particles harboring E5 protein. Moreover, the cell supernatants were prepared from DCs pulsed with TCL-E5. Their immunological responses and antitumor effects were investigated in a mouse model. The TCL-E5-pulsed DCs regimen could direct immunity toward Th1 and CTL responses, leading to tumor volume reduction and high percentage of tumor-free mice. The TCL-pulsed DCs regimen could not induce significant antitumor effects compared to TCL-E5-pulsed-DCs regimen indicating main role of E5 in vaccine development.