DOI: 10.5061/dryad.dr33q
title: Data from: Faster clonal turnover in high-infection habitats provides evidence for parasite-mediated selection

description: According to the Red Queen hypothesis for sex, parasite-mediated selection
 against common clones counterbalances the reproductive advantage of
 asexual lineages, which would otherwise outcompete sexual conspecifics.
 Such selection on the clonal population is expected to lead to a faster
 clonal turnover in habitats where selection by parasites is stronger. We
 tested this prediction by comparing the genetic structure of clonal and
 sexual populations of freshwater snail Potamopyrgus antipodarum between
 years 2003 and 2007 in three depth-specific habitats in Lake Alexandrina
 (South Island, New Zealand). These habitats differ in the risk of
 infection by castrating trematodes and in the relative proportion of
 sexual individuals. As predicted, we found that the clonal structure
 changed significantly in shallow and mid-water habitats, where prevalence
 of infection was high, but not in the deep habitat, where parasite
 prevalence was low. Additionally, we found that both clonal diversity and
 evenness of the asexual population declined in the shallow habitat. In
 contrast, the genetic structure (based on F–statistics) of the coexisting
 sexual population did not change, which suggests that the change in the
 clonal structure cannot be related to genetic changes in the sexual
 population. Finally, the frequency of sexuals had no effect on the
 diversity of the sympatric clonal population. Taken together, our results
 show a more rapid clonal turnover in high-infection habitats, which gives
 support for the Red Queen hypothesis for sex.
description: Allozyme genotypes at 9
 loci and ploidy information for Potamopyrgus antipodarum
 snailsAllozyme genotypes at 9
 loci and ploidy information for Potamopyrgus antipodarum snails from Lake
 Alexandrina, New Zealand. Genotypes were obtained with celulose acetate
 electrophoresis. Ploidy level was assigned based on asymetric banding
 pattern in heterozygotes. Loci used for assigning ploidy level include:
 6PGD, MPI, IDH2, PGM1 and PGM2 (ploidy assignment based on AAT1, AAT2,
 IDH1 and PEP-D was not reliable). Description of the column headings and
 abbreviations uded: Data in columns A, B, C, D was not used in the
 analyses, but can be used to track the sample back to one of the original
 celulose acetate gels that are stored at the department of Aquatic
 Ecology, Eawag, Switzerland. A-SNAIL - snail number per sample B-TUBE -
 eppendorf tube code C-RUN - allozyme genotyping run D-POSITION - position
 on gel E-YEAR - year sampled F-SITE - sampling site G-HABITAT - habitat
 where sample was taken (3 depth-related habitats: shallow, mid-water and
 deep) H-PLOIDY - ploidy level (NA - not available, 0 - homozygote,
 2-diploid, 3-triploid) I- SEX_COMMON_RARE - reproductive mode, for clones
 common or rare (S- sexuals, C-common clones, R-rare clones). A clone was
 defined as ‘common’ if its frequency among triploid asexuals was above
 1.5% in any of the samples (year/site/habitat combination) J-CLONE_ID -
 Unique clone number K-S - column names are allozyme loci names. Genotypes
 are coded with a single digit. Loci for which the banding pattern is
 unreliable for asigning ploidy level are left with 2 alleles for triploid
 heterozygotes.dryad JEB-12310
 genotypes.xlsx