DOI: 10.5061/dryad.4rd14
title: Data from: Mosquito saliva increases endothelial permeability in the skin, immune cell migration, and dengue pathogenesis during antibody-dependent enhancement

description: Dengue remains the most prevalent arthropod-borne viral disease in humans.
 While probing for blood vessels, Aedes aegypti and Ae. albopictus
 mosquitoes transmit the four serotypes of dengue virus (DENV1-4) by
 injecting virus-containing saliva into the skin. Even though arthropod
 saliva is known to facilitate transmission and modulate host responses to
 other pathogens, the full impact of mosquito saliva on dengue pathogenesis
 is still not well understood. Inoculating mice lacking the interferon-α/β
 receptor intradermally with DENV revealed that mosquito salivary gland
 extract (SGE) exacerbates dengue pathogenesis specifically in the presence
 of enhancing serotype-cross-reactive antibodies—when individuals already
 carry an increased risk for severe disease. We further establish that SGE
 increases viral titers in the skin, boosts antibody-enhanced DENV
 infection of dendritic cells and macrophages in the dermis, and amplifies
 dendritic cell migration to skin-draining lymph nodes. We demonstrate that
 SGE directly disrupts endothelial barrier function in vitro and induces
 endothelial permeability in vivo in the skin. Finally, we show that
 surgically removing the site of DENV transmission in the skin after 4
 hours rescued mice from disease in the absence of SGE, but no longer
 prevented lethal antibody-enhanced disease when SGE was present. These
 results indicate that SGE accelerates the dynamics of dengue pathogenesis
 after virus transmission in the skin and induces severe antibody-enhanced
 disease systemically. Our study reveals novel aspects of dengue
 pathogenesis and suggests that animal models of dengue and pre-clinical
 testing of dengue vaccines should consider mosquito-derived factors as
 well as enhancing antibodies.
description: Inventory of data
 repositorySchmid et al. –
 Inventory of data repositorySchmid Inventory data
 repository SGE.pdf01 - Dermis steady
 stateFile type: The
 repository includes representative FSC 3.0 files that were exported from
 Diva software (BD Biosciences) and can be opened with FlowJo software
 (TreeStar). Fluorescent channels also indicate the specifically stained
 markers. Compensations values between fluorescence channels are included
 within FCS files.01_Dermis_steady
 state.fcs02 - Dermis 14h
 PBSFile type: The
 repository includes representative FSC 3.0 files that were exported from
 Diva software (BD Biosciences) and can be opened with FlowJo software
 (TreeStar). Fluorescent channels also indicate the specifically stained
 markers. Compensations values between fluorescence channels are included
 within FCS files.02_Dermis_14h_PBS.fcs03 - Dermis 14h SGEFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.03_Dermis_14h_SGE.fcs04 - Dermis 14h primary DENVFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.04_Dermis_14h_primary_DENV.fcs05 - Dermis 14h primary DENV+SGEFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.05_Dermis_14h_primary_DENV+SGE.fcs06 - Dermis 14h ADE DENVFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.06_Dermis_14h_ADE_DENV.fcs07 - Dermis 14h ADE DENV+SGE.fcsFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.07_Dermis_14h_ADE_DENV+SGE.fcs08 - LNs steady stateFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.08_LNs_steady state.fcs09 - LNs 14h PBSFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.09_LNs_14h_PBS.fcs10 - LNs 14h SGEFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.10_LNs_14h_SGE.fcs11 - LNs 14h primary DENVFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.11_LNs_14h_primary_DENV.fcs12 - LNs 14h primary DENV+SGEFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.12_LNs_14h_primary_DENV+SGE.fcs13 - LNs 14h ADE DENVFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.13_LNs_14h_ADE_DENV.fcs14 - LNs 14h ADE DENV+SGEFile type: The repository includes representative FSC 3.0 files that were exported from Diva software (BD Biosciences) and can be opened with FlowJo software (TreeStar). Fluorescent channels also indicate the specifically stained markers. Compensations values between fluorescence channels are included within FCS files.14_LNs_14h_ADE_DENV+SGE.fcs