cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14288606.v1
title: COMPARISON OF AGGLUTINATING AND GROWTH INHIBITION ANTIBODIES LEVELS IN SOWS IMMUNIZED WITH TWO EXPERIMENTAL INACTIVATED MONOVALENT VACCINES OF CANICOLA SEROVAR: WHOLE CULTURE WITH ALUMINUM HYDROXIDE AND LIPOPOLYSACCHARIDE SUBUNIT WITH LIPID A

description: ABSTRACT It was compared the antibody response of sows immunized with two experimental vaccines produced with L.interrogans, serovar Canicola, strain LO-4, isolated in Brazil.One of the vaccines was the usual bacterin (whole culture inactivated with phenol and adjuvanted with aluminum hydroxide -WC-AlOH3) and the other one was a subunit vaccine produced with a lipopolysaccharide (LPS) fraction extracted from the bacteria outer envelop and with the lipid A, also extracted from the leptospira wall as adjuvant (LPS-MPLA). Experiment was as follows: group 1 (n = 11), not immunized control, group 2, (n = 11): two immunization with 30 days interval of LPS-MPLA vaccine and group 3 (n = 11): two immunization with 30 days interval of WC-AlOH3 vaccine All three groups were simultaneously immunized, independently of pregnancy stage. Both agglutinin and neutralizing post vaccination antibodies levels were measured respectively by the microscopic sera agglutination with live antigens test (MAT) and the in vitro leptospira growth inhibition test (GIT). Sera collections were performed each 30 days during four months after the first vaccination. Non vaccinated control group animals presented no agglutinating antibodies against Canicola serovar during the whole experiment. At 32 and 68 post vaccination days the agglutinating antibodies levels of group 2 (LPS-MPLA) were significantly higher than the observed in group 3 (WC AlOH3), respectively, p = 0.013 and p = 0.031. The differences observed in the growth inhibition antibodies titers of the two vaccines tested were not significant (p &gt; 0.05). Despite the peak of post-vaccination agglutinins have been registered at 68 days after first immunization, higher levels growth inhibition antibodies were detected at 30 days of first vaccination. Subunit vaccine presented the same immunogenic capacity for the production of neutralizing antibodies as the whole culture one.
DOI: 10.6084/m9.figshare.14288599.v1
title: LISTERIA MONOCYTOGENES: OCCURRENCE IN DAIRY PRODUCTS AND ITS IMPLICATIONS IN PUBLIC HEALTH

description: ABSTRACT Listeria monocytogenes is the causative agent of listeriosis, a serious foodborne disease that promotes severe human infections with high mortality rates. Milk and byproducts are among the food products most often involved in the transmission of L. monocytogenes. Listeriosis mainly affects immunodepressed individuals, pregnant women, neonates and the elderly, thus emphasizing its opportunistic character and importance to public health. The present article presents a narrative and critical review concerning the risk to human health from the consumption of dairy products contaminated with L. monocytogenes. Also, a discussion is made on the factors that determine the contamination by L. monocytogenes in the production and distribution chain of milk and dairy products. The available data on the occurrence of L. monocytogenes in raw milk and dairy products in Brazil are also presented and evaluated, taking into consideration its potential for involvement in human listeriosis outbreaks. Additionally, this review indicates the main research and work areas needed for the prevention of L. monocytogenes contamination in dairy products.
DOI: 10.6084/m9.figshare.14288597.v1
title: CULTIVATION OF PLEUROTUS SAJOR-CAJUIN DIFFERENT PASTEURIZED SUBSTRATES

description: ABSTRACT CULTIVATION OF PLEUROTUS SAJOR-CAJU IN DIFFERENT PASTEURIZED SUBSTRATES. This research was aimed at evaluating the viability of the Pleurotus sajor-caju crop using various residues generated in the region of Pelotas, state of Rio Grand do Sul, Brazil. The substrates were formulated with sugarcane bagasse, elephant grass and rice straw, separate and mixed, for a total of 7 treatments. The substrates were moistened for 24 hours, pasteurized at 100º C for 30 minutes, and then seeded with a 3% spawn of P. sajor-caju (PSC96/03) and placed into 1 kg polyethylene bags. The incubation was carried out at 25-28º C, for 25 days, and the harvest took place in two flushes after periods of 45 days each. The mushrooms were collected and weighed for the evaluation of the fresh mass (g), productivity (%) and biological efficiency (%). The results showed that the rice straw produces more in the first flush and the elephant grass more in the second, while the overall totals for fresh mass and productivity of both flushes showed that each of these materials was, separately, a viable substrate for the cultivation of P. sajor-caju mushrooms. The sugarcane bagasse proved to be the least indicated substrate for the cultivation of this mushroom by means of this technique.
DOI: 10.6084/m9.figshare.14288595.v1
title: MICROBIOLOGICAL QUALITY OF “MINAS FRESCAL” CHEESE MARKETED IN SANTA HELENA, PR, BRAZIL

description: ABSTRACT From May 2008 to April 2009 the microbiological quality of 40 samples of artisan-made cheeses and cheeses inspected by the State Inspection Service and Federal in Santa Helena, state of Paraná, Brazil, was evaluated by quantifying the most probable number of thermotolerant coliforms, count of Staphylococcus spp., research of yeasts and molds, and the determination of the presence/absence of Salmonella spp. and Listeria monocytogenes. In regard to the presence of thermotolerant coliforms, 90% of the artisan-made samples and 55% of inspected samples did not meet the standards established by ANVISA under Resolution Nº 12 dated 2 January 2001. In regard to the count of Staphylococcus spp., 100% of the artisan-made samples and 25% of the inspected samples did not meet the legal standards. Salmonella spp. and L. monocytogenes were not isolated in 25 g of the product, showing that for these agents the cheese samples analyzed, both the artisan made as well as the inspected production, were within the established standards (absence in 25 g of the product). The results revealed that of all the samples analyzed, only 15% were in accordance with the standards established by ANVISA, with a high percentage of artisan-made (100%) and inspected cheese (70%) outside the legal limits. Therefore, health authorities should pay more attention to this sector, since such products may endanger the health of consumers.
DOI: 10.6084/m9.figshare.14288593.v1
title: LEAF, STEM AND ROOT ABSORPTION OF PYMETROZINE AND FLONICAMID TO CONTROL THE COTTON APHID APHIS GOSSYPII GLOVER, 1877 (HEMIPTERA: APHIDIDAE)

description: ABSTRACT Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) is a cotton pest, and if not controlled can reduce yield and fiber quality. The discovery of new chemical groups of insecticides brings new perspectives to IPM; pymetrozine and flonicamid are examples of new launched pesticides. This study was carried out to evaluate the efficacy of these two insecticides to control A. gossypii when they were absorbed by cotton root, stem and leaves. The statistical design was a randomized factorial (2 x 3) with five replications in blocks. Each plot consisted of a pot with two plants, kept under greenhouse conditions, in 2005. We evaluated the population density of aphids on the leaves during sixteen days after treatment. The results show: (1) flonicamid has efficiency when absorbed by stem and leaves, (2) pymetrozine efficacy is through foliar uptake, (3) the root absorption do not provide satisfactory control for both insecticides, and, (4) both insecticides control the aphid-cotton when is applied on leaves.
DOI: 10.6084/m9.figshare.14288591.v1
title: MICROBIOLOGICAL CHARACTERISTICS OF WHOLE MILK AND DAIRY DRINK PROCESSED BY UHT (ULTRA-HIGH TEMPERATURE) DURING ITS SHELF LIFE

description: ABSTRACT UHT milk and dairy drinks are submitted to the same heat treatment, but they are different products, since the addition of up to 50% of whey is allowed in dairy drinks. The aim of this study was to evaluate the microbiological characteristics of these products in relation to current legislation, during the course of their shelf life. In the present study, 150 samples, 75 of each type of product, from 5 different national brands were analyzed. The 15 samples of each brand were separated into 3 lots containing 5 samples each, analyzed at the beginning, middle and end of the validity period. Upon microbiological analysis all UHT milk samples were in conformance with current legislation. After the samples were opened and chilled for 48 hours, 26.6% of them presented an increased psychrotrophic population. The dairy drink results showed that 12% of the samples were out of conformance with the current legislation. After the samples were opened and chilled for 48 hours, 36% of them presented an increased psychrotrophic population. These results should be considered as an alert in regard to the need for rigorous inspection of UHT milk and dairy drink products.
DOI: 10.6084/m9.figshare.14288589.v1
title: SUSCEPTIBILITY TO ANTIMICROBIAL DRUGS AND PRODUCTION OF BETA-LACTAMASE BY STAPHYLOCOCCUS STRAINS ISOLATED FROM MASTITIS IN GOATS IN THESEMI-ARID REGION OF PARAIBA STATE, BRAZIL

description: ABSTRACT The present study was aimed to test the in vitro antimicrobial susceptibility using disc diffusion assay and minimum inhibitory concentration (MIC) values, as well as the production of beta-lactamase of 42 strains of Staphylococcus sp. isolated from subclinical mastitis in goats in the semi-arid region of Paraiba state, in Brazil. To this end, 14 samples of negative Staphylococcus chromogenes, 10 S. waneri, 6 S. saprophyticus, 5 S. aureus, 3 S. epidermidis, 2 S. intermedius and 2 Staphylococcus were used. Regarding the in vitro susceptibility, the highest levels of resistance observed concerned penicillin (66.67%) and ampicillin (63.89%), and the greatest sensitivity observed concerned Cefoxitin (97.22%). Multiple resistance to 3 or more antimicrobial drugs was observed in 42.86% of the isolates. Of the total Staphylococcus isolated, 73.81% were positive for the beta-lactamase production test. The minimum inhibitory concentration showed 100% of the samples susceptible to norfloxacin, and the maximum resistance values to the other antimicrobial drugs were 30%. According to the results obtained, it is concluded that, in order to select the most appropriate drug to treat mastitis in goats caused by strains of Staphylococcus, the antimicrobial sensitivity profile of the strain must be known and routine laboratory tests for detecting beta-lactamase should be performed because these enzymes are disseminated both in human and veterinary medicine. The high resistance to penicillin and ampicillin demonstrates the limitation in the use of antibiotics in the treatment of mastitis in goats. Although in the present study the cephalosporines demonstrated a considerable degree of sensitivity, the use of these antimicrobial agents in veterinary medicine becomes a matter of concern due to the incidence of strains that produce betalactamase.
DOI: 10.6084/m9.figshare.14288588.v1
title: SURVIVAL OF WORKERS OF THE TERMITE CORNITERMES CUMULANS (KOLLAR, 1832) (ISOPTERA: TERMITIDAE) FED WITH DIFFERENT DIETS

description: ABSTRACT We evaluated the survival of the termite Cornitermes cumulans (Kollar, 1832), isolated from the colony and kept on different artificial diets in the laboratory (21 ± 1° C, 90 ± 10% UR and 24h of scotophase), in Lavras, Minas Gerais, Brazil. Fifty termite workers were isolated in each plastic Petri dish, where they were offered diets or not. We used 6 replicates per treatment, evaluating the daily survival of termites until the death of all the individuals. The results were submitted to Weibull survival analysis and the equations were subjected to the test of identity of non-linear models. There was a difference in estimated survival of termites treated with different diets. It was concluded that it is possible to keep Cornitermes cumulans workers alive and isolated from the colony with the use of a diet containing filter paper, bagasse cane sugar and agar as a food source.
DOI: 10.6084/m9.figshare.14288585.v1
title: SEROPREVALENCE AND RISK FACTORS ASSOCIATED TO NEOSPORA CANINUM IN FEMALE BOVINES FROM THE WESTERN SÃO PAULO STATE, BRAZIL

description: ABSTRACT The prevalence of anti-Neospora caninum antibodies was evaluated in 1104 cows from 118 farms in the Western region of São Paulo State, Brazil, using indirect immunofluorescence antibody assay. Sixty-five (55.0%; CI 95%: 46.0-63.8%) farms showed at least one seropositive animal. Prevalence over 10% was observed in 33 farms (28.0%; CI 95%: 20.4-36.5%), and over 20% in 18 farms (15.2%; CI 95%: 9.5-22.6%). Prevalence of animals showed 120 (10.9%) positive females (IFAT ? 1:100). The positivity in the farms showed significant association with farms producing more than 50 liters per day. Infection caused by the protozoan N. caninum occurs in the Western region of the state of São Paulo, and although no differences were observed either in relation to the production type or management practices, greater milk yield was related to greater prevalence of infection.
DOI: 10.6084/m9.figshare.14288583.v1
title: MYCELIAL KINETICS OF EDIBLE FUNGI PLEUROTUS OSTREATUS AND LENTINULA EDODES IN LIGNOCELLULOSIC RESIDUES

description: ABSTRACT The objective of this work was to evaluate the mycelial growth of 2 edible fungi (Pleurotus ostreatus and Lentinula edodes) in 6 culture media [(malt-agar, sawdust-dextrose-agar-marupá (SDA-MA), sawdust-dextrose-agar-cajuí (SDA-CA), sawdust-dextrose-agar-açaí (SDA-AÇA), sawdust-dextrose-agar-banana 50% (BAN 50%) and sawdust-dextrose-agar-banana 100% (BAN 100%)], in Petri dishes. The experimental design was totally randomized, in a 6x2 factorial scheme. Each treatment consisted of six repetitions in 1 Petri dish, totaling 72 experimental units. It was verified that P. ostreatus presented better mycelial development (81.00; 64.66; 81.00; 50.16 and 33.33mm for SDA-MA, SDA-CA, SDA-AÇA, BAN 50% and BAN 100%, respectively) than L. edodes (32.00; 31.66; 27.66; 37.33 and 21.83mm for SDA-MA, SDA-CA, SDA-AÇA, BAN 50% and BAN 100%, respectively). It was also verified that there was no advantage for L. edodes in relation to mycelial growth, when media based on residues were used, compared to malt-agar medium (control), which obtained the best performance (62.17mm). As for P. ostreatus, SDA-MA and SDA-AÇA medium presented the highest growth averages (81 mm), representing a growth increase of 34% in relation to the control medium (malt-agar), whose growth average was 60.33mm. Thus, the residues tested present potential to be used in fungiculture, especially for the cultivation of P. ostreatus.
DOI: 10.6084/m9.figshare.14288580.v1
title: INTERACTION BETWEEN TRANSGENICS AND INSECTICIDES IN THE CONTROL OF KEY PESTS ON OFF-SEASON MAIZE HYBRIDS

description: ABSTRACT Diverse lepidopteran pests attack the maize crop. The commercialization of hybrids of transgenic Bt maize, integrating in its genome the gene Cry1Ab, from Bacillus thuringiensis subsp. kurstaki, which codifies the protein with toxic effect on insects of the Lepidoptera order, has been recently introduced in Brazil. The objective of this work was to evaluate, under field conditions, the effectiveness of conventional and transgenic maize hybrids for the control of pests, sprayed or not with insecticides. The assay was carried out in an experimental area of the Polo Apta Centro Norte, in Pindorama, São Paulo State, Brazil. Five conventional maize hybrids were evaluated in comparison with the respective isogenic Bt hybrids. The evaluation was carried out every 10 days, in 20 plants of the 2 central lines of the parcel for Spodoptera frugiperda (Smith) damage. For damages of Helicoverpa zea (Bod.) and Diatraea saccharalis (Fab.) the evaluations were carried out in the pre-harvest. The interactions between the hybrids and the transgeny occurred in all evaluations starting at the 40th day after emergence (DAE). The Bt hybrids evaluated presented the lowest scores for visual S. frugiperda damages in all the evaluations. The transgenic maize also provided the lowest percentage of cobs damaged by H. zea and S. frugiperda, and of stalks attacked by D. saccharalis. In conclusion, the transgenic maize provided reduction in visual damages caused by the pests, however, it presented different performance among the hybrids.
DOI: 10.6084/m9.figshare.14288578.v1
title: IN VITRO INHIBITION OF FUNGAL PLANT PATHOGENS BY DIFFERENT CONCENTRATIONS OF MEDICINAL PLANTS EXTRACTS

description: ABSTRACT In order to evaluate the in vitro effect of different concentrations of plant extracts on the growth of Cercospora kikuchii. Fusarium solani. Colletotrichum sp. and Phomopsis sp., 4 bioassays experiments were conducted at the Plant Pathology Laboratory of the Federal University of Grande Dourados, Brazil. The experimental design was a completely randomized design in a 3 x 6 factorial arrangement, with 8 replicates for each bioassay. Aqueous extracts of garlic, cinnamon and clove were evaluated in concentrations of 0, 0.5, 1, 5, 10 and 20%. Extracts were obtained through maceration of 30 g of plant material in 120 mL of distilled water. The extracts were filtered in grade-1 Whatman paper and incorporated into PDA medium to obtain the desired concentrations. Subsequently, the solution was poured into Petri dishes, and then 3 mm-diameter-PDA plugs with pathogen mycelium were transferred to the center of the PDA + plant extract Petri dish. Plates were incubated at 25° C with a 12-h-day photoperiod. Fungal growth was performed daily by measuring the colony diameter. The results showed that the effect of aqueous extract of cinnamon for all pathogens studied, and garlic extract for F. solani. Colletotrichum sp. and Phomopsis sp., was dependent on the concentrations used, noting a higher antifungal activity with increased concentrations used. Clove extract presented the greatest effectiveness for the control of the plant pathogens tested, with observed suppression on the growth of Colletotrichum sp. C. kikuchii. F. solani and Phomopsis sp. at the concentrations 7.4, 7.5, 8.9 and 7.0%, respectively. The aqueous extract of garlic at a concentration of 9.7% presented effective control only for C. kikuchii.
DOI: 10.6084/m9.figshare.14288576.v1
title: REACTION OF YELLOW PASSION FRUIT TO MELOIDOGYNE INCOGNITA RACE 3

description: ABSTRACT This study concerned the reaction of yellow passion fruit ‘Maguary’ and ‘Afruvec’ to the phytonematode Meloidogyne incognita race 3 in greenhouse conditions. An entirely randomized experimental design with 3 treatments (‘Maguary’, ‘Afruvec’, and tomato cv. ‘Rutgers’) and 4 repetitions was used, each plot consisting of 1 vase containing 1 plant. After 6 months, an evaluation was made of the index of galls and egg mass in the yellow passion fruit varieties and in the tomato cv. ‘Rutgers’. The classification of resistance to the phytonematode was made by criterion of the reproduction factor (RF). ‘Maguary’ presented a zero index of galls and egg mass, while ‘Afruvec’ showed a low index of galls and egg mass in relation to the tomato cv. Rutgers. According to the RF, ‘Maguary’ was characterized as immune to the phytonematode, while ‘Afruvec’ was resistant, and the tomato cv. ‘Rutgers’ was susceptible.
DOI: 10.6084/m9.figshare.14288574.v1
title: IN VITRO ACTIVITY OF THE HYDROALCOHOLIC EXTRACT OF LIPPIA SIDOIDES CHAM ON BUOYANT LARVAE OF THIRD-STAGE GASTROINTESTINAL NEMATODES (FAMILY TRICHOSTRONGYLIDAE) OF GOATS

description: ABSTRACT The in vitro larvicidal activity of the hydroalcoholic extract of rosemary pepper (Lippia sidoides Cham) was evaluated on buoyant larvae of third-stage gastrointestinal gastrointestinal nematodes of goats. The results demonstrated that the concentration of 500 mg mL-1 presented effective activity, with a capacity of action of 95.89%, thus demonstrating the possibility of anthelmintic therapeutic use for this extract.
DOI: 10.6084/m9.figshare.14288573.v1
title: EFFECT OF DIFFERENT HERBICIDES, DOSES AND SPRAY VOLUME IN THE CHEMICAL MANAGEMENT OF PEARL MILLET [PENNISETUM GLAUCUM (L. LEEK)]

description: ABSTRACT The objective of this work was to evaluate the efficacy of different herbicides applied in post-emergence in the desiccation of pearl millet with different doses and spray volumes. For the desiccation, doses at 0, 25, 50 and 100% of the herbicide glyphosate (1,440 g ha-1) were used in 3 formulations (Original Round up, Round up Transorb and Round up WG), paraquat + diuron (500 + 250 g ha-1) and glufosinate-ammonium (800 g ha-1). In all treatments, 2 spray volumes (200 and 400 l ha-1) were used, and there was 1 treatment (control) without the application of herbicides. The treatment effects were evaluated visually at 3, 7, 14, 21 and 28 days after application (DAA). At the end of the evaluations, the dry mass of the plants was determined. The field trial was carried out in randomized block design. At the end of the study, at 28 DAA, all treatments presented total control of plant pearl millet, regardless of the dose and spray volume, except the treatments with paraquat + diuron and glufosinate-ammonium at the lower dose (25%), in 2 spray volumes, which nevertheless showed good and very good control, these two herbicides presenting an effect of volume, in which the control was higher with the use of smaller volume.
DOI: 10.6084/m9.figshare.14288567.v1
title: TRICHOGRAMMA SPP. (HYMENOPTERA: TRICHOGRAMMATIDAE) SPECIES AND STRAINS FOR THE CONTROL OF ANTICARSIA GEMMATALIS (LEPIDOPTERA

description: ABSTRACT Several Trichogramma (Hymenoptera: Trichogrammatidae) species parasitize Anticarsia gemmatalis (Lepidoptera: Noctuidae) eggs, the major soybean desfoliator in Brazil. The objective of this work was to evaluate the parasitism of eleven Trichogramma species/strains in cardboards with twenty eggs of A. gemmatalis by this parasitoid female for 24 hours. The strain trat - 2 Trichogramma atopovirilia (Hymenoptera: Trichogrammatidae) of Sete Lagoas, Minas Gerais, showed better performance with 19 A. gemmatalis eggs parasitized by female in 24 hours, 98% viability and sex ratio of 0.84. The strain Trichogramma pretiosum of Crystalline, Goiás, parasitized 11.9 A. gemmatalis eggs per female with 98% viability and sex ratio of 0.65. This work confirms the importance of evaluating Trichogramma strains before biological control programs, because different strains of the same species of parasitoid have distinct results.
DOI: 10.6084/m9.figshare.14288564.v1
title: BOVINE BRUCELOSIS IN THE STATE OF PARAÍBA: A RETROSPECTIVE SURVEY

description: ABSTRACT The aim of this study was to determine the frequency of positive herds (foci) and seropositive animals for bovine brucellosis in the state of Paraíba, Northeast region of Brazil. Data from the Agency of Agricultural Protection in the state, collected from its 23 microregions, during the period from January 2008 to July 2009, were used. During this period, 11,149 herds were examined, and 55,691 cattle sera were submitted to the diagnosis of brucellosis. For serological diagnosis the rose bengal test was used. A herd was considered a focus when it presented at least one seropositive animal. Of the herds investigated, 104 (0.93%) had at least one seropositive animal, and of the animals examined, 199 (0.36%) were seropositive. There was a significant difference (p &lt; 0.001) in the proportion of seropositivity for females (0.47%) and males (0.04%).