DOI: 10.5061/dryad.c6jh2
title: Data from: Seeking signatures of reinforcement at the genetic level: a hitchhiking mapping and candidate gene approach in the house mouse

description: Reinforcement is the process by which prezygotic isolation is strengthened
 as a response to selection against hybridization. Most empirical support
 for reinforcement comes from the observation of its possible phenotypic
 signature: an accentuated degree of prezygotic isolation in the hybrid
 zone as compared to allopatry. Here, we implemented a novel approach to
 this question by seeking for the signature of reinforcement at the genetic
 level. In the house mouse, selection against hybrids and enhanced
 olfactory-based assortative mate preferences are observed in a hybrid zone
 between the two European subspecies Mus musculus musculus and M. m.
 domesticus, suggesting a possible recent reinforcement event. To test for
 the genetic signature of reinforcing selection and identify genes involved
 in sexual isolation, we adopted a hitchhiking mapping approach targeting
 genomic regions containing candidate genes for assortative mating in mice.
 We densely scanned these genomic regions in hybrid zone and allopatric
 samples using a large number of fast evolving microsatellite loci that
 allow the detection of recent selection events. We found a handful of loci
 showing the expected pattern of significant reduction in variability in
 populations close to the hybrid zone, showing assortative odour preference
 in mate choice experiments as compared to populations further away and
 displaying no such preference. These loci lie close to genes that we
 pinpoint as testable candidates for further investigation.
description: Primer_informationThis file provides information for all primers used in this study.Behavioural dataThis file provides behavioural raw data (mate choice tests). In particular, it provides for each actor of the choice the individual IDs (sample_ID1 and sample_ID2), the subspecies of origin, the sex, the geographical origin (field "Sample"), the trapping site ID with its geographical coordinates (in degrees), the time spent sniffing each stimulus (musculus or domesticus urinary signals), and the difference in time spent sniffing the two stimuli.Genotyping_raw_data_main_experimentThis file provides all raw genotyping data for the main experiment (1248 loci). For each primer pair and sample, the different rows represent the different peaks present in the pool before any filtering was applied. For each peak, peak size, peak height and peak area is provided.Genotyping_raw_data_for_HI_estimationThis file provides genotyping data of the 21 diagnostic markers (insertion/deletion polymorphisms of transposable elements, columns C to W) used to estimate the hybrid index of each individual used in this study. More information on the markers used and applied genotyping procedure can be found in Latour et al 2015 Proceeedings of the Royal Society of London.Genotyping_raw_data_pooling_validation_experimentThis file provides all genotyping raw data for the pooling strategy validation experiment. Sheet 1 shows individual raw genotypes (14 individuals typed separately) and sheet 2 shows the pool raw genotypes.Genotyping_raw_data_outlier_validation_experimentThis file provides raw genotyping results for individual typing of outlier microsatellite loci.