cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/jwj-0n3-4yb
title: Propebela nobilis (Møller, 1842)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ktw-a72-r7j
title: Oliva vidua (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ffz-nbp-j4y
title: Mangelia coarctata (Forbes, 1840)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s45-fj8-jle
title: Liguus fasciatus (O. F. Müller, 1774)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/1xf-phe-21v
title: Mazatlania fulgurata (R. A. Philippi, 1846)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/2l5-b4l-a5e
title: Brachypodella raveni (Crosse, 1872)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gjt-sf0-ljg
title: Turridae H. Adams & A. Adams, 1853 (1838)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jyb-qpm-gfl
title: Macrogastra densestriata (Rossmässler, 1836)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/clk-1a9-8sy
title: Siphonaria zelandica Quoy & Gaimard, 1833

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/faa-bcq-vqn
title: Steironepion moniliferum (G. B. Sowerby I, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0hp-1vt-mja
title: Columbellidae Swainson, 1840

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/b5b-lg3-vtx
title: Mitrella ocellata (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/rjl-zck-24a
title: Radix Montfort, 1810

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/pf7-k9s-f35
title: Achatinella byronii rugosa Newcomb, 1854

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/r3a-910-cr1
title: Biomphalaria pfeifferi (Krauss, 1848)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/czt-ejh-22p
title: Olivella nivea (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s67-ywt-638
title: Oliva vidua (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.11588/duerer.online.689f2400ec848
title: Albrecht Dürer kommt in die Lehre zu Michael Wolgemut, https://d-nb.info/gnd/1209509652
DOI: 10.3535/bhg-65k-r1c
title: Tetrentodon gracillimus (Poey, 1852)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s95-h87-41x
title: Strigillaria vetusta (Rossmässler, 1836)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4sp-etj-xxf
title: Odostomella doliolum (R. A. Philippi, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/5b6-0tj-srk
title: Crassispira Swainson, 1840

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/k1d-8ld-1tx
title: Clausilia bidentata (Strøm, 1765)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/03r-kx3-5xv
title: Pseudoliva crassa (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/r9r-g8x-j95
title: Eratoidea sulcata (A. d'Orbigny, 1842)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/dre-yy2-pvb
title: Marginellidae J. Fleming, 1828

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/shz-1nq-ge0
title: Conus arenatus Hwass, 1792

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fmf-g2h-0tr
title: Tonicia atrata (G. B. Sowerby II, 1839)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/6kl-h2h-dft
title: Cochlodina orthostoma (Menke, 1828)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/h01-lgj-q68
title: Mocella elliottae (Climo, 1969)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fxr-345-0kf
title: Conus biliosus (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/n7v-ql5-39k
title: Macularia sylvatica (Draparnaud, 1801)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4ne-ns2-9kk
title: Euplecta imperforata Schepman, 1918

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0gh-7ej-jwh
title: Lima lima (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/dd4-zh6-kgn
title: Cantareus apertus (Born, 1778)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ls3-574-415
title: Otala Schumacher, 1817

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/rwv-46s-65j
title: Sphincterochila cyrenaica cyrenaica Sturany, 1908

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fsd-2y9-y5e
title: Lunarca ovalis (Bruguière, 1789)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/r04-8rv-kac
title: Anadara secticostata (Reeve, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/2hn-3v0-9zc
title: Rumina decollata (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/51z-v8e-xag
title: Octopus Cuvier, 1798

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/alh-5aj-3mz
title: Xeromunda durieui (L. Pfeiffer, 1848)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/cf6-bqa-0jl
title: Trochoidea trochoides (Poiret, 1789)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fkp-ts4-7wt
title: Stenopylis coarctata (Möllendorff, 1894)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.11588/duerer.online.689f240fc32f6
title: Dürers Vermählung mit Agnes Frey, 177703
DOI: 10.3535/h01-w5s-s6v
title: Conus cumingii Reeve, 1848

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/m1y-53e-w1z
title: Discartemon collingei (Sykes, 1902)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/99y-e32-p7l
title: Euglandina rosea (A. Férussac, 1821)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/1hp-h04-vr3
title: Hygromia limbata (Draparnaud, 1805)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/j05-5ws-dkm
title: Conus flavidus Lamarck, 1810

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0fn-5nr-zpd
title: Aegopinella pura (Alder, 1830)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/cvh-9sh-qpj
title: Pecten fumatus Reeve, 1852

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/wbj-qws-581
title: Conus figulinus Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/8g3-bh3-4fg
title: Pteria sterna (A. Gould, 1851)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/df2-0rq-m06
title: Kaliella microconus (Mousson, 1865)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/38l-txw-yrf
title: Amphidromus perversus rufocinctus Fruhstorfer, 1905

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/v0j-nqh-0l1
title: Botula fusca (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yc7-mxz-n90
title: Conus fergusoni G. B. Sowerby II, 1873

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ypk-twv-xc4
title: Pleurodonte guadeloupensis (Pilsbry, 1889)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/639-znn-zyz
title: Metafruticicola pellita (A. Férussac, 1832)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ehh-bwh-99w
title: Polydontes Montfort, 1810

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/xq3-45r-qmf
title: Urticicola umbrosus (C. Pfeiffer, 1828)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/7wa-pxn-408
title: Metafruticicola noverca (L. Pfeiffer, 1853)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jns-9vw-d11
title: Nucula sulcata Bronn, 1831

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.11588/duerer.online.689f241ad02b5
title: Albrecht Dürer wird von den Malern zu Antwerpen festlich bewirtet, 177873
DOI: 10.3535/qbg-d0q-k8j
title: Leptaxis groviana groviana (A. Férussac, 1832)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/een-1lr-4pl
title: Hemitrochus varians (Menke, 1829)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jgp-9zs-4pc
title: Cerion uva (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/63k-zza-1n5
title: Larkinia grandis (Broderip & G. B. Sowerby I, 1829)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/n6c-c5l-kwn
title: Marmorana serpentina (A. Férussac, 1821)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/rta-qcm-m8z
title: Crassostrea rhizophorae (Guilding, 1828)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/t78-xj3-tvz
title: Caucasotachea vindobonensis (C. Pfeiffer, 1828)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/z2d-ezb-j33
title: Cernuella virgata (Da Costa, 1778)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9dj-45v-5td
title: Conus capitaneus Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/btf-zk7-kmv
title: Hyotissa hyotis (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9th-ekc-q7j
title: Mytilus edulis Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/xy5-y0n-27y
title: Xeropicta ledereri mavromoustakisi (F. Haas, 1933)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jm1-2nt-f5s
title: Argonauta nodosus [Lightfoot], 1786

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/4de-ryy-v96
title: Euhadra quaesita (Deshayes, 1850)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yr5-zkh-b5r
title: Zonites smyrnensis (J. R. Roth, 1839)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.6075/j0mk6bkx
title: CIL:13748, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library

description: In the absence of boi (boi[e] mutant), Hedgehog (Hh, red) is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13744, 13745). Expression of Boi in apical cells relocalizes Hh to apical cells (CIL#13746). Apical cell expression of Ihog, which also binds Hh directly, rescues Hh localization in the boi[e] mutant (this image; boi[e]; ihog[EP]/+; babGal4/+). Germ cells are identified with anti-Vasa (blue). Image is Fig 3E in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
description: Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). Primary antibodies were 1:2,000 rabbit anti-Vasa; 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.). Secondary antibodies used were FITC, Cy3, and/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0c53k16
title: CIL:12638, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: High resolution image of quick-freeze deep-etch rotary-shadowed replica of an early endosome with two clathrin-coated evaginations. Presumably these coated pits contain receptors for cargo that will be pinched off from the early endosomes and passed on through carrier vesicles to the late endosomal compartments. Early endosomes lie at the level of the intraciliary lattice and proximal ends of basal bodies, but internal to the striated bands. TEM taken on 6/4/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Adapted with permission from J. Cell Sci. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.6075/j00p0xst
title: CIL:11835, Potorous tridactylus, epithelial cell. In Cell Image Library

description: Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tubulin and observed by fluorescence microscopy. Epifluorescence
image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 1 of associated manuscript (J Cell Biol, 161:845-851, 2003).
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation
DOI: 10.6075/j0d21wbg
title: CIL:13746, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells in the boi[e] mutant (boi[e]; UAS-Boi/+; babGal4/+) rescues Hh localization to apical cells (this image). However, expression of a Boi mutant lacking the Hh-binding domain does not (CIL# 13747). Nuclei are labeled (DRAQ5, blue). Image is Fig 3C in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
description: Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). For anti-Boi immunostaining, ovaries were fixed in 2% formaldehyde on ice. For nuclear staining, fixed ovaries were incubated for 15 min with Draq5 (Cell Signaling Technology). Primary antibodies were 1:50 rat anti-Boi (Hartman et al., 2010); 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.) or 1:100 rabbit anti-Hh (Ballet et al. 2003). Secondary antibodies used were FITC and Cy3 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); Cy3; membrane-permeant probe; DRAQ5
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j02j69jr
title: CIL:13745, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library

description: Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (this image). Expression of Boi in apical cells in the boi[e] mutant rescues Hh localization to apical cells (CIL# 13746); however expression of a Boi mutant that lacks the Hh binding domain does not (CIL# 13747). Image is Fig 3B in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
description: Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). For anti-Boi immunostaining, ovaries were fixed in 2% formaldehyde on ice. Primary antibodies were 1:50 rat anti-Boi (Hartman et al., 2010); 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.) or 1:100 rabbit anti-Hh (Ballet et al. 2003). Secondary antibodies used were FITC, Cy3, and/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j0cj8d62
title: CIL: 53956, Homo sapiens, HeLa. In Cell Image Library

description: Visualization methods: confocal microscopy
description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: RGC family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.