cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.5849105
title: Dataset related to the article: "Netrin-1 in Atherosclerosis: Relationship between Human Macrophage Intracellular Levels and In Vivo Plaque Morphology"

description: This record contains raw data related to the article: "Netrin-1 in Atherosclerosis: Relationship between Human Macrophage Intracellular Levels and In Vivo Plaque Morphology" Abstract: Netrin-1 is a laminin-like protein that plays a pivotal role in cell migration and, according to the site of its release, exerts both pro and anti-atherosclerotic functions. Macrophages, key cells in atherosclerosis, are heterogeneous in morphology and function and different subpopulations may support plaque progression, stabilization, and/or regression. Netrin-1 was evaluated in plasma and, together with its receptor UNC5b, in both spindle and round monocyte-derived macrophages (MDMs) morphotypes from coronary artery disease (CAD) patients and control subjects. In CAD patients, plaque features were detected in vivo by optical coherence tomography. CAD patients had lower plasma Netrin-1 levels and a higher MDMs expression of both protein and its receptor compared to controls. Specifically, a progressive increase in Netrin-1 and UNC5b was evidenced going from controls to stable angina (SA) and acute myocardial infarction (AMI) patients. Of note, spindle MDMs of AMI showed a marked increase of both Netrin-1 and its receptor compared to spindle MDMs of controls. UNC5b expression is always higher in spindle compared to round MDMs, regardless of the subgroup. Finally, CAD patients with higher intracellular Netrin-1 levels showed greater intraplaque macrophage accumulation in vivo. Our findings support the role of Netrin-1 and UNC5b in the atherosclerotic process.
description: This research was funded by the Italian Ministry of Health , Rome, Italy. grant number RC 2017 ID 2633356-BIO 65. Co-funding is provided by the contribution of the Italian "5 x 1000 tax
DOI: 10.5281/zenodo.5849104
title: Dataset related to the article: "Netrin-1 in Atherosclerosis: Relationship between Human Macrophage Intracellular Levels and In Vivo Plaque Morphology"

description: This record contains raw data related to the article: "Netrin-1 in Atherosclerosis: Relationship between Human Macrophage Intracellular Levels and In Vivo Plaque Morphology" Abstract: Netrin-1 is a laminin-like protein that plays a pivotal role in cell migration and, according to the site of its release, exerts both pro and anti-atherosclerotic functions. Macrophages, key cells in atherosclerosis, are heterogeneous in morphology and function and different subpopulations may support plaque progression, stabilization, and/or regression. Netrin-1 was evaluated in plasma and, together with its receptor UNC5b, in both spindle and round monocyte-derived macrophages (MDMs) morphotypes from coronary artery disease (CAD) patients and control subjects. In CAD patients, plaque features were detected in vivo by optical coherence tomography. CAD patients had lower plasma Netrin-1 levels and a higher MDMs expression of both protein and its receptor compared to controls. Specifically, a progressive increase in Netrin-1 and UNC5b was evidenced going from controls to stable angina (SA) and acute myocardial infarction (AMI) patients. Of note, spindle MDMs of AMI showed a marked increase of both Netrin-1 and its receptor compared to spindle MDMs of controls. UNC5b expression is always higher in spindle compared to round MDMs, regardless of the subgroup. Finally, CAD patients with higher intracellular Netrin-1 levels showed greater intraplaque macrophage accumulation in vivo. Our findings support the role of Netrin-1 and UNC5b in the atherosclerotic process.
description: This research was funded by the Italian Ministry of Health , Rome, Italy. grant number RC 2017 ID 2633356-BIO 65. Co-funding is provided by the contribution of the Italian "5 x 1000 tax
DOI: 10.48370/ofd/doah6g/ys8lrm
title: Claviceps purpurea (Fries) Tulasne F-1823.tif

description: :unav
DOI: 10.48370/ofd/doah6g
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 1823

description: Fungarium Mikromycetes
DOI: 10.5281/zenodo.5845429
title: Dataset related to the article: "Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation"

description: This record containsraw data related to the article: "Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation" Abstract: The exposure of human endothelial cells to 3-morpholinosydnonimine (SIN-1) induced the<br> expression of cyclooxygenase-2 (COX-2) in a dose- and time-dependent manner. Interestingly, after a<br> prolonged incubation (&gt;8 h) several proteoforms were visualized by Western blot, corresponding<br> to different states of glycosylation of the protein. This effect was specific for SIN-1 that generates<br> peroxynitrite and it was not detected with other nitric oxide-donors. Metabolic labeling experiments<br> using 35S or cycloheximide suggested that the formation of hypoglycosylated COX-2 was dependent<br> on de novo synthesis of the protein rather than the deglycosylation of the native protein. Moreover,<br> SIN-1 reduced the activity of the hexokinase, the enzyme responsible for the first step of glycolysis.<br> The hypoglycosylated COX-2 induced by SIN-1 showed a reduced capacity to generate prostaglandins<br> and the activity was only partially recovered after immunoprecipitation. Finally, hypoglycosylated<br> COX-2 showed a more rapid rate of degradation compared to COX-2 induced by IL-1a and an<br> alteration in the localization with an accumulation mainly detected in the nuclear membrane. Our<br> results have important implication to understand the effect of peroxynitrite on COX-2 expression<br> and activity, and they may help to identify new pharmacological tools direct to increase COX-2<br> degradation or to inhibit its activity.
description: This work was supported by the Italian Ministry of Health, Rome, Italy. Ricerca Corrente RC 2019 MPP1A ID 2755301
DOI: 10.5281/zenodo.5845428
title: Dataset related to the article: "Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation"

description: This record containsraw data related to the article: "Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation" Abstract: The exposure of human endothelial cells to 3-morpholinosydnonimine (SIN-1) induced the<br> expression of cyclooxygenase-2 (COX-2) in a dose- and time-dependent manner. Interestingly, after a<br> prolonged incubation (&gt;8 h) several proteoforms were visualized by Western blot, corresponding<br> to different states of glycosylation of the protein. This effect was specific for SIN-1 that generates<br> peroxynitrite and it was not detected with other nitric oxide-donors. Metabolic labeling experiments<br> using 35S or cycloheximide suggested that the formation of hypoglycosylated COX-2 was dependent<br> on de novo synthesis of the protein rather than the deglycosylation of the native protein. Moreover,<br> SIN-1 reduced the activity of the hexokinase, the enzyme responsible for the first step of glycolysis.<br> The hypoglycosylated COX-2 induced by SIN-1 showed a reduced capacity to generate prostaglandins<br> and the activity was only partially recovered after immunoprecipitation. Finally, hypoglycosylated<br> COX-2 showed a more rapid rate of degradation compared to COX-2 induced by IL-1a and an<br> alteration in the localization with an accumulation mainly detected in the nuclear membrane. Our<br> results have important implication to understand the effect of peroxynitrite on COX-2 expression<br> and activity, and they may help to identify new pharmacological tools direct to increase COX-2<br> degradation or to inhibit its activity.
description: This work was supported by the Italian Ministry of Health, Rome, Italy. Ricerca Corrente RC 2019 MPP1A ID 2755301
DOI: 10.48370/ofd/sy2ajp/dcgkjl
title: Claviceps purpurea (Fries) Tulasne F-1874 (5).tif

description: :unav
DOI: 10.48370/ofd/sy2ajp/15uwn2
title: Claviceps purpurea (Fries) Tulasne F-1874.tif

description: :unav
DOI: 10.48370/ofd/sy2ajp/a8cuca
title: Claviceps purpurea (Fries) Tulasne F-1874 (2).tif

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DOI: 10.48370/ofd/sy2ajp/gl96lo
title: Claviceps purpurea (Fries) Tulasne F-1874 (3).tif

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DOI: 10.48370/ofd/sy2ajp/wmm7ib
title: Claviceps purpurea (Fries) Tulasne F-1874 (4).tif

description: :unav
DOI: 10.48370/ofd/sy2ajp
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 1874

description: Fungarium Mikromycetes
DOI: 10.48370/ofd/j77sqg/wf2lqa
title: Claviceps purpurea (Fries) Tulasne F-2018 (3).tif

description: :unav
DOI: 10.48370/ofd/j77sqg/y3kzza
title: Claviceps purpurea (Fries) Tulasne F-2018 (5).tif

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DOI: 10.48370/ofd/j77sqg/d1fxce
title: Claviceps purpurea (Fries) Tulasne F-2018 (4).tif

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DOI: 10.48370/ofd/j77sqg/q13z2n
title: Claviceps purpurea (Fries) Tulasne F-2018 (2).tif

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DOI: 10.48370/ofd/j77sqg/m0584g
title: Claviceps purpurea (Fries) Tulasne F-2018.tif

description: :unav
DOI: 10.48370/ofd/j77sqg
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 2018

description: Fungarium Mikromycetes
DOI: 10.5281/zenodo.5930480
title: Dataset related to the article "Persistent long-term platelet activation and endothelial perturbation in women with Takotsubo syndrome"

description: This record contains raw data related to the article "Persistent long-term platelet activation and endothelial perturbation in women with Takotsubo syndrome" <strong>Background: </strong>Takotsubo (TTS) syndrome is an acute cardiac condition characterized by transient and reversible left ventricle dysfunction that mainly affects postmenopausal women. Catecholamine burst is the most accredited mechanism underpinning TTS onset and leading to endothelial dysfunction and platelet activation. Even if the use of low dose acetylsalycilic acid (ASA) in this clinical setting is based on both clinical presentation and unfavorable long-term prognosis, its efficacy has been recently challenged. <strong>Aim: </strong>This study was designed to assess endothelial function, residual thromboxane formation and platelet aggregation in TTS women on low-dose ASA treatment at long-term follow-up. <strong>Methods: </strong>Twenty-eight females with previously diagnosis of TTS syndrome were enrolled. Data were compared to those obtained from 23 coronary artery disease (CAD) women with a history of acute myocardial infarction, and 26 control subjects with no TTS or clinically evident CAD. Psychological and clinical profile were assessed in all study groups at the enrollment. Main metabolites involved in L-arginine/nitric oxide pathway, urinary prostacyclin, serum and urine thromboxane metabolites were measured by LCMS/MS methods. Thrombomodulin levels were quantified using an ELISA kit, and platelet aggregation, carried out on platelet rich-plasma, was induced by ADP or by epinephrine (EPI), norepinephrine (NORE) and TRAP-6, alone or in association with ADP and evaluated by Born's method. <strong>Results: </strong>In TTS women an endothelial derangement, characterized by reduced citrulline production and increased thrombomodulin concentration, with no perturbation in prostacyclin levels, was evidenced. In addition, despite ASA treatment, TTS displayed a higher residual thromboxane formation, in parallel with an enhanced platelet response to compared to CAD. <strong>Conclusions: </strong>Our study highlighted the presence of endothelial perturbation in TTS patients even at long-term from the index event. The residual thromboxane production and platelet aggregation still leave open the question about the use of low dose ASA in this clinical setting.
description: This work was supported by funding from the Italian Ministry of Health, Rome, Italy (Ricerca Corrente RC 2018 CI 1.5 ID 2634621, 2019 MPP 2B ID 2755316).
DOI: 10.5281/zenodo.5930479
title: Dataset related to the article "Persistent long-term platelet activation and endothelial perturbation in women with Takotsubo syndrome"

description: This record contains raw data related to the article "Persistent long-term platelet activation and endothelial perturbation in women with Takotsubo syndrome" <strong>Background: </strong>Takotsubo (TTS) syndrome is an acute cardiac condition characterized by transient and reversible left ventricle dysfunction that mainly affects postmenopausal women. Catecholamine burst is the most accredited mechanism underpinning TTS onset and leading to endothelial dysfunction and platelet activation. Even if the use of low dose acetylsalycilic acid (ASA) in this clinical setting is based on both clinical presentation and unfavorable long-term prognosis, its efficacy has been recently challenged. <strong>Aim: </strong>This study was designed to assess endothelial function, residual thromboxane formation and platelet aggregation in TTS women on low-dose ASA treatment at long-term follow-up. <strong>Methods: </strong>Twenty-eight females with previously diagnosis of TTS syndrome were enrolled. Data were compared to those obtained from 23 coronary artery disease (CAD) women with a history of acute myocardial infarction, and 26 control subjects with no TTS or clinically evident CAD. Psychological and clinical profile were assessed in all study groups at the enrollment. Main metabolites involved in L-arginine/nitric oxide pathway, urinary prostacyclin, serum and urine thromboxane metabolites were measured by LCMS/MS methods. Thrombomodulin levels were quantified using an ELISA kit, and platelet aggregation, carried out on platelet rich-plasma, was induced by ADP or by epinephrine (EPI), norepinephrine (NORE) and TRAP-6, alone or in association with ADP and evaluated by Born's method. <strong>Results: </strong>In TTS women an endothelial derangement, characterized by reduced citrulline production and increased thrombomodulin concentration, with no perturbation in prostacyclin levels, was evidenced. In addition, despite ASA treatment, TTS displayed a higher residual thromboxane formation, in parallel with an enhanced platelet response to compared to CAD. <strong>Conclusions: </strong>Our study highlighted the presence of endothelial perturbation in TTS patients even at long-term from the index event. The residual thromboxane production and platelet aggregation still leave open the question about the use of low dose ASA in this clinical setting.
description: This work was supported by funding from the Italian Ministry of Health, Rome, Italy (Ricerca Corrente RC 2018 CI 1.5 ID 2634621, 2019 MPP 2B ID 2755316).
DOI: 10.48370/ofd/phjhmr/stlj1b
title: Claviceps purpurea (Fries) Tulasne F-0907.tif

description: :unav
DOI: 10.48370/ofd/phjhmr/hjtqql
title: Claviceps purpurea (Fries) Tulasne F-0907 (4).tif

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DOI: 10.48370/ofd/phjhmr/oida4v
title: Claviceps purpurea (Fries) Tulasne F-0907 (2).tif

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DOI: 10.48370/ofd/phjhmr/ctvgzk
title: Claviceps purpurea (Fries) Tulasne F-0907 (5).tif

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DOI: 10.48370/ofd/phjhmr/yd47hj
title: Claviceps purpurea (Fries) Tulasne F-0907 (3).tif

description: :unav
DOI: 10.48370/ofd/phjhmr
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 907

description: Fungarium Mikromycetes
DOI: 10.5281/zenodo.5907192
title: Dataset related to the article "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure"

description: This record contains raw data related to the article "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure" Abstract Heart failure (HF) is a complex disease due to the intricate interplay of several mechanisms, which therefore implies the need for a multimarker strategy to better personalize the care of patients with HF. In this study, we developed a targeted mass spectrometry approach based on multiple reaction monitoring (MRM) to measure multiple circulating protein biomarkers, involved in cardiovascular disease, to address their relevance in the human HF, intending to assess the feasibility of the workflow in the disease monitoring and risk stratification. In this study, we analyzed a total of 60 plasma proteins in 30 plasma samples from eight control subjects and 22 age and gender- matched HF patients. We identified a panel of four plasma proteins, namely<br> Neuropilin-2, Beta 2 microglobulin, alpha-1-antichymotrypsin, and complement component C9, that were more abundant in HF patients in relation to disease severity and pulmonary dysfunction.<br> Moreover, we showed the ability of the combination of these candidate proteins to discriminate, with sufficient accuracy, HF patients from healthy subjects. In conclusion, we demonstrated the feasibility and potential of a proteomic workflow based on MRM mass spectrometry for the evaluation of multiple proteins in human plasma and the identification of a panel of biomarkers of HF severity.
description: This work was supported by the Italian Ministry of Health, Rome, Italy (Ricerca Corrente RC 2019 MPP1A ID 2755301).
DOI: 10.48370/ofd/b5hmxn/v4bkpv
title: Claviceps purpurea (Fries) Tulasne F-0624.tif

description: :unav
DOI: 10.5281/zenodo.5907191
title: Dataset related to the article "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure"

description: This record contains raw data related to the article "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure" Abstract Heart failure (HF) is a complex disease due to the intricate interplay of several mechanisms, which therefore implies the need for a multimarker strategy to better personalize the care of patients with HF. In this study, we developed a targeted mass spectrometry approach based on multiple reaction monitoring (MRM) to measure multiple circulating protein biomarkers, involved in cardiovascular disease, to address their relevance in the human HF, intending to assess the feasibility of the workflow in the disease monitoring and risk stratification. In this study, we analyzed a total of 60 plasma proteins in 30 plasma samples from eight control subjects and 22 age and gender- matched HF patients. We identified a panel of four plasma proteins, namely<br> Neuropilin-2, Beta 2 microglobulin, alpha-1-antichymotrypsin, and complement component C9, that were more abundant in HF patients in relation to disease severity and pulmonary dysfunction.<br> Moreover, we showed the ability of the combination of these candidate proteins to discriminate, with sufficient accuracy, HF patients from healthy subjects. In conclusion, we demonstrated the feasibility and potential of a proteomic workflow based on MRM mass spectrometry for the evaluation of multiple proteins in human plasma and the identification of a panel of biomarkers of HF severity.
description: This work was supported by the Italian Ministry of Health, Rome, Italy (Ricerca Corrente RC 2019 MPP1A ID 2755301).
DOI: 10.48370/ofd/b5hmxn
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 624

description: Fungarium Mikromycetes
DOI: 10.48370/ofd/9qbz6i/58hvwi
title: Claviceps purpurea (Fries) Tulasne F-4382.tif

description: :unav
DOI: 10.48370/ofd/9qbz6i
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 4382

description: Fungarium Mikromycetes
DOI: 10.5281/zenodo.5927661
title: Dataset related to the article "Red Blood Cell Morphodynamics: A New Potential Marker in High-Risk Patients"

description: This record contains raw data related to the article "Red Blood Cell Morphodynamics: A New Potential Marker in High-Risk Patients" In the last years, a substantial contribution of red blood cells (RBCs) in cardiovascular homeostasis has been evidenced, as these cells are able to regulate cardiovascular function by the export of adenosine triphosphate and nitric oxide as well as to maintain redox balance through a well-developed antioxidant system. Recently a link between high-risk plaque (HRP) features and myocardial ischemia, in the absence of severe lumen stenosis, has been evidenced. Nonobstructive coronary artery disease (nonob CAD) has been associated in fact with a greater 1-year risk of myocardial infarction and all-cause mortality compared with no apparent CAD. This new evidence increases interest in searching new triggers to identify these high-risk patients, in the absence/or on top of traditional hazard markers. In this study, we investigated the existence of any association between RBC morphodynamics and HRP features in individuals with different grades of coronary stenosis detected by coronary computed tomography angiography (CCTA). Ninety-one consecutive individuals who underwent CCTA [33 no CAD; 26 nonobstructive (nonob), and 32 obstructive (ob) CAD] were enrolled. RBC morphodynamic features, i.e., RBC aggregability and deformability, were analyzed by means of Laser Assisted Optical Rotation Cell Analyzer (LoRRca MaxSis). The putative global RBC morphodynamic (RMD) score and the related risk chart, associating the extent of HRP (e.g., the non-calcified plaque volume) with both the RMD score and the max % stenosis were computed. In nonob CAD group only positive correlations between RBC rigidity, osmotic fragility or aggregability and HRP features (plaque necrotic core, fibro-fatty and fibro-fatty plus necrotic core plaque volumes) were highlighted. Interestingly, in this patient cohort three of these RBC morphodynamic features result to be independent predictors of the presence of non-calcified plaque volume in this patients group. The risk chart created shows that only in nonob CAD plaque vulnerability increases according to the score quartile. Findings of this work, by evidencing the association between erythrocyte morphodynamic characteristics assessed by LoRRca and plaque instability in a high-risk cohort of nonob CAD, suggest the use of these blood cell features in the identification of high-risk patients, in the absence of severe coronary stenosis.
description: This research was funded by Italian Ministry of Health, grant number RC-2016 BIO03.
DOI: 10.48370/ofd/ywsigv/ceaaxz
title: Claviceps purpurea (Fries) Tulasne F-1893.tif

description: :unav
DOI: 10.48370/ofd/ywsigv
title: Buławinka czerwona (Claviceps purpurea (Fries) Tulasne), 1893

description: Fungarium Mikromycetes