DOI: 10.22002/fqpb4-tfs14
title: Hylemonella gracilis

description: Hylemonella gracilis.  The rest of the files are in ~/Images/060913.  The analysis consists of motor picking.  Some ctf correction.
description: <strong>Tilt Series Date:</strong> 2006-09-13</p>
<strong>Data Taken By:</strong> Gavin Murphy</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 60.0°), step: 0.9°, constant angular increment, dosage: 75.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> Cells provided by Jared Leadbetter; Data collected and processed by Gavin Murphy</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in dilute media without salt.  0.5 g/L of Tryptone, 0.5 g/L YE. Cells grown for 2 days.  OD never gets above 0.005.  Centrifuged lightly e.g. 3500x g, then concentrated 50-100x fold.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity...</p>
  H16_060913_tilt.jpg: * associated to rawdata H16_060913_rec.jpg: file associated to 3D Image #85 A16CTFrec0.jpg: file associated to 3D Image #85
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gme2006-09-13-3/rawdata/</p>A16_S474G3_34kx12d75eCHalfsec.mrc,  Tilt Series (Pixel Size 0.67 nm), 964.8 MB  
        
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        A16NoCTFrec0.27.mrc,  Reconstruction (Pixel Size 0.0 nm), 473.9 MB  
        
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        A16NoCTFhigh_44_part.pif,  Reconstruction (Pixel Size 0.0 nm), 5.5 MB  
        
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        A16CTFrec0.42_113.tif,  file associated to 3D Image #85, 2.4 MB  
        
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        A16CTFrec0.42_149.tif,  file associated to 3D Image #85, 2.4 MB  
        
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        A16CTFrec0.42_XZ443.tif,  file associated to 3D Image #85, 2.1 MB  
        
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        A16CTFrec0.42_YZ508.tif,  file associated to 3D Image #85, 2.1 MB  
        
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        gm2006-09-13-3ribosegnew.am,  Ribosome segmentation of a subvolume containing the whole cell.am file created from post-processed Molmatch data.Created by David J. Rosenman, 1.0 MB  
        
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