cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.23090401
title: COFFEE TERRITORIALIZATION IN CAPARAÓ CAPIXABA

description: Abstract Brazil has several regions shaped by the insertion of the coffee economy, an arrangement that highlights the coffee production chain as a structuring element of the territory. This article aims to characterize the territorialization of the coffee production chain in the Caparaó Capixaba region. The qualitative, exploratory, descriptive, and ethnographic research is divided into three stages: bibliographic, fieldwork and interviews. The field research lasted from November 2020 to January 2021. The interviews, conducted online, occurred between September and November 2021. The respondents were 15 coffee producers in the municipalities of Iúna and Irupi, in Caparaó Capixaba, Brazil. Consequently, the formation of Caparaó territoriality from the region's historic coffee culture is evident. Thus, the study increases the understanding of the production, consumption, and commercialization of the region's coffee. Some essential factors considered are the appropriation of space by producers, resignification of practices and territory through work, reinforcement of local identity, formation of the coffee landscape and subsequent productive differentiation through the focus on specialty coffees. Finally, visibility is given to coffee growers' protagonism in a region neglected in recent decades due to low quantitative productivity but which boasts expressive results in agroecological, family and specialty coffee production.
DOI: 10.6084/m9.figshare.23090401.v1
title: COFFEE TERRITORIALIZATION IN CAPARAÓ CAPIXABA

description: Abstract Brazil has several regions shaped by the insertion of the coffee economy, an arrangement that highlights the coffee production chain as a structuring element of the territory. This article aims to characterize the territorialization of the coffee production chain in the Caparaó Capixaba region. The qualitative, exploratory, descriptive, and ethnographic research is divided into three stages: bibliographic, fieldwork and interviews. The field research lasted from November 2020 to January 2021. The interviews, conducted online, occurred between September and November 2021. The respondents were 15 coffee producers in the municipalities of Iúna and Irupi, in Caparaó Capixaba, Brazil. Consequently, the formation of Caparaó territoriality from the region's historic coffee culture is evident. Thus, the study increases the understanding of the production, consumption, and commercialization of the region's coffee. Some essential factors considered are the appropriation of space by producers, resignification of practices and territory through work, reinforcement of local identity, formation of the coffee landscape and subsequent productive differentiation through the focus on specialty coffees. Finally, visibility is given to coffee growers' protagonism in a region neglected in recent decades due to low quantitative productivity but which boasts expressive results in agroecological, family and specialty coffee production.
DOI: 10.6084/m9.figshare.23090434
title: Effect of NPK fertilizer rates and growth regulator concentrations on sweet potato crop yield

description: ABSTRACT The low mean yield of sweet potato crops in Brazil is related to several inadequate crop management practices. Considering the increasing production of this vegetable, the objective of this work was to evaluate the yield response of sweet potato crops subjected to different NPK fertilizer rates (NPK) combined with different growth regulator concentrations. The experiment was carried out in a randomized block design, with a 5×5 factorial arrangement consisted of 5 NPK fertilizer rates (0, 696, 1044, 1392, and 1740 kg ha-1) combined with 5 growth regulator concentrations (0, 20, 25, 30, and 35 mL L-1). Numbers of total and commercial roots per plant were affected by the factors tested, presenting a linear increase as the rates and concentrations were increased. The highest estimated sweet potato yield was 43 Mg ha -1, which was obtained using a NPK rate of 1522.64 kg ha-1 and a growth regulator concentration of 34.69 mL L-1. The higher NPK rates improved water use efficiency up to a maximum value of 8.1 kg m-3 with application of the NPK rate of 1740 kg ha-1. After determining a fixed yield level, the production factors tested act as imperfect substitutes in some intervals of variation of the inputs.
DOI: 10.6084/m9.figshare.23090434.v1
title: Effect of NPK fertilizer rates and growth regulator concentrations on sweet potato crop yield

description: ABSTRACT The low mean yield of sweet potato crops in Brazil is related to several inadequate crop management practices. Considering the increasing production of this vegetable, the objective of this work was to evaluate the yield response of sweet potato crops subjected to different NPK fertilizer rates (NPK) combined with different growth regulator concentrations. The experiment was carried out in a randomized block design, with a 5×5 factorial arrangement consisted of 5 NPK fertilizer rates (0, 696, 1044, 1392, and 1740 kg ha-1) combined with 5 growth regulator concentrations (0, 20, 25, 30, and 35 mL L-1). Numbers of total and commercial roots per plant were affected by the factors tested, presenting a linear increase as the rates and concentrations were increased. The highest estimated sweet potato yield was 43 Mg ha -1, which was obtained using a NPK rate of 1522.64 kg ha-1 and a growth regulator concentration of 34.69 mL L-1. The higher NPK rates improved water use efficiency up to a maximum value of 8.1 kg m-3 with application of the NPK rate of 1740 kg ha-1. After determining a fixed yield level, the production factors tested act as imperfect substitutes in some intervals of variation of the inputs.
DOI: 10.6084/m9.figshare.23090443
title: Carbohydrase inclusion in a corn-soybean diet improves broiler growth performance

description: ABSTRACT. An experiment was conducted to evaluate the effect of diets with reduced energy level content, supplemented with carbohydrase, on broiler performance and the coefficient of metabolizability of nutrients. A total of 720 one-day-old male Cobb-500 chicks were distributed in a completely randomized design, with six treatments, eight repetitions of 15 birds each. The treatments were: (1) a positive control, basal diet to meet the requirements of broiler chickens (PC); (2) a negative control, basal diet with a reduction of 80 kcal kg-1 (NC); (3) NC + alphagalactosydase; (4) NC + xylanase; (5) NC + xylanase and alphagalactosydase, and (6) NC + enzymatic blend (alphagalactosydase, xylanase, pectinase and amylase). The nutrient digestibility was not improved by use of enzymes. At 7 days of age, the broilers which were fed diets supplemented with enzymes showed a lower feed intake (FI) and better feed conversion ratio (FCR) than the broilers fed on PC. Both the NC and enzymatic blend resulted in a worse performance of the broilers at 21, 35 and 42 days old. The use of alphagalactosydase and xylanase, isolated or in combination, in a corn-soybean meal-based diet is effective in improving the growth performance of broilers fed energy-reduced diets.
DOI: 10.6084/m9.figshare.23090443.v1
title: Carbohydrase inclusion in a corn-soybean diet improves broiler growth performance

description: ABSTRACT. An experiment was conducted to evaluate the effect of diets with reduced energy level content, supplemented with carbohydrase, on broiler performance and the coefficient of metabolizability of nutrients. A total of 720 one-day-old male Cobb-500 chicks were distributed in a completely randomized design, with six treatments, eight repetitions of 15 birds each. The treatments were: (1) a positive control, basal diet to meet the requirements of broiler chickens (PC); (2) a negative control, basal diet with a reduction of 80 kcal kg-1 (NC); (3) NC + alphagalactosydase; (4) NC + xylanase; (5) NC + xylanase and alphagalactosydase, and (6) NC + enzymatic blend (alphagalactosydase, xylanase, pectinase and amylase). The nutrient digestibility was not improved by use of enzymes. At 7 days of age, the broilers which were fed diets supplemented with enzymes showed a lower feed intake (FI) and better feed conversion ratio (FCR) than the broilers fed on PC. Both the NC and enzymatic blend resulted in a worse performance of the broilers at 21, 35 and 42 days old. The use of alphagalactosydase and xylanase, isolated or in combination, in a corn-soybean meal-based diet is effective in improving the growth performance of broilers fed energy-reduced diets.
DOI: 10.6084/m9.figshare.23090446.v1
title: Hematological and coagulation parameters as predictors of death by Coronavirus disease in hospitalized patients: a Brazilian follow-up study

description: Abstract This study aimed to evaluate the hematological and coagulation parameters according to the clinical outcomes of coronavirus disease (COVID-19). We analyzed the hematological and coagulation parameters of hospitalized patients with COVID-19 at admission, and two and three weeks during hospitalization. To assess the performance of these parameters in predicting poor outcomes, receiver operating characteristic (ROC) curves were created. We studied 128 patients with COVID-19 (59.2±17.7 years, 56% male). Non-survivors (n=54, 42%) presented significant alterations in hematological and coagulation parameters at admission, such as increased in white blood cells (WBC), neutrophil, and band cell counts, as well as elevated prothrombin time (PT), activated partial thromboplastin time, and D-dimer levels. During follow-up, the same group presented a gradual increase in D-dimer and PT levels, accompanied by a reduction in PT activity, hemoglobin, and red blood cell count (RBC). ROC curves showed that WBC, neutrophil, and band cell counts presented the best area under the curve (AUC) values with sensitivity and specificity of &gt;70%; however, a logistic regression model combining all the parameters, except for RBC, presented an AUC of 0.89, sensitivity of 84.84%, and specificity of 77.41%. Our study shows that significant alterations in hematological and coagulation tests at admission could be useful predictors of disease severity and mortality in COVID-19.
DOI: 10.6084/m9.figshare.23090446
title: Hematological and coagulation parameters as predictors of death by Coronavirus disease in hospitalized patients: a Brazilian follow-up study

description: Abstract This study aimed to evaluate the hematological and coagulation parameters according to the clinical outcomes of coronavirus disease (COVID-19). We analyzed the hematological and coagulation parameters of hospitalized patients with COVID-19 at admission, and two and three weeks during hospitalization. To assess the performance of these parameters in predicting poor outcomes, receiver operating characteristic (ROC) curves were created. We studied 128 patients with COVID-19 (59.2±17.7 years, 56% male). Non-survivors (n=54, 42%) presented significant alterations in hematological and coagulation parameters at admission, such as increased in white blood cells (WBC), neutrophil, and band cell counts, as well as elevated prothrombin time (PT), activated partial thromboplastin time, and D-dimer levels. During follow-up, the same group presented a gradual increase in D-dimer and PT levels, accompanied by a reduction in PT activity, hemoglobin, and red blood cell count (RBC). ROC curves showed that WBC, neutrophil, and band cell counts presented the best area under the curve (AUC) values with sensitivity and specificity of &gt;70%; however, a logistic regression model combining all the parameters, except for RBC, presented an AUC of 0.89, sensitivity of 84.84%, and specificity of 77.41%. Our study shows that significant alterations in hematological and coagulation tests at admission could be useful predictors of disease severity and mortality in COVID-19.
DOI: 10.6084/m9.figshare.23090454
title: Selective photodynamic effects on cervical adenocarcinoma cells provided by F127 Pluronic®-based micelles modulating hypericin delivery

description: Abstract Cervical cancer is a leading cause of death among women. The endocervical adenocarcinoma (ECA) represents an aggressive and metastatic type of cancer with no effective treatment options currently available. We evaluated the antitumoral and anti-migratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against a human cell line derived from invasive cervical adenocarcinoma (HeLa) compared to a human epithelial cell line (HaCaT). The phototoxicity and cytotoxicity of F127/HYP were evaluated by the following assays: colorimetric assay, MTT, cellular morphological changes by microscopy and long-term cytotoxicity by clonogenic assay. In addition, we performed fluorescence microscopy to analyze cell uptake and subcellular distribution of F127/HYP, cell death pathway and reactive oxygen species (ROS) production. The PDT mechanism was determined with sodium azide and D-mannitol and cell migration by wound-healing assay. The treatment with F127/HYP promoted a phototoxic result in the HeLa cells in a dose-dependent and selective form. Internalization of F127/HYP was observed mainly in the mitochondria, causing cell death by necrosis and ROS production especially by the type II PDT mechanism. Furthermore, F127/HYP reduced the long-term proliferation and migration capacity of HeLa cells. Overall, our results indicate a potentially application of F127/HYP micelles as a novel approach for PDT with HYP delivery to more specifically treat ECA.
DOI: 10.6084/m9.figshare.23090454.v1
title: Selective photodynamic effects on cervical adenocarcinoma cells provided by F127 Pluronic®-based micelles modulating hypericin delivery

description: Abstract Cervical cancer is a leading cause of death among women. The endocervical adenocarcinoma (ECA) represents an aggressive and metastatic type of cancer with no effective treatment options currently available. We evaluated the antitumoral and anti-migratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against a human cell line derived from invasive cervical adenocarcinoma (HeLa) compared to a human epithelial cell line (HaCaT). The phototoxicity and cytotoxicity of F127/HYP were evaluated by the following assays: colorimetric assay, MTT, cellular morphological changes by microscopy and long-term cytotoxicity by clonogenic assay. In addition, we performed fluorescence microscopy to analyze cell uptake and subcellular distribution of F127/HYP, cell death pathway and reactive oxygen species (ROS) production. The PDT mechanism was determined with sodium azide and D-mannitol and cell migration by wound-healing assay. The treatment with F127/HYP promoted a phototoxic result in the HeLa cells in a dose-dependent and selective form. Internalization of F127/HYP was observed mainly in the mitochondria, causing cell death by necrosis and ROS production especially by the type II PDT mechanism. Furthermore, F127/HYP reduced the long-term proliferation and migration capacity of HeLa cells. Overall, our results indicate a potentially application of F127/HYP micelles as a novel approach for PDT with HYP delivery to more specifically treat ECA.
DOI: 10.6084/m9.figshare.23090461
title: Counterfactual analysis of income distribution in Brazil

description: ABSTRACT Using an input-output framework, this article studies the consequences of income redistribution from rich people either to poor people or to the government upon the sectoral structure of the Brazilian economy in the 70’s. Besides the traditional use of elements of sectoral analysis, the main concept used to achieve this goal is that of “subeconomies”, focusing on industries (and associated patterns of employment and value added) which produce the components of particular expenditure bundles. Thus, a subeconomy is composed by the economic activity derived from determined expenditure groups, such as poor consumer households, government, etc. Two main results emerge from the empirical application of these concepts. First, agriculture is the most important sector in the generation of employment in response to transfers of income from rich to poor households. Second, the urbanization which accompanied growth during the 70’s favours a redistributive strategy in which the role of government becomes outstanding in terms of generation of both value added and employment.
DOI: 10.6084/m9.figshare.23090461.v1
title: Counterfactual analysis of income distribution in Brazil

description: ABSTRACT Using an input-output framework, this article studies the consequences of income redistribution from rich people either to poor people or to the government upon the sectoral structure of the Brazilian economy in the 70’s. Besides the traditional use of elements of sectoral analysis, the main concept used to achieve this goal is that of “subeconomies”, focusing on industries (and associated patterns of employment and value added) which produce the components of particular expenditure bundles. Thus, a subeconomy is composed by the economic activity derived from determined expenditure groups, such as poor consumer households, government, etc. Two main results emerge from the empirical application of these concepts. First, agriculture is the most important sector in the generation of employment in response to transfers of income from rich to poor households. Second, the urbanization which accompanied growth during the 70’s favours a redistributive strategy in which the role of government becomes outstanding in terms of generation of both value added and employment.
DOI: 10.6084/m9.figshare.23090470
title: Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease

description: BACKGROUND Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity. OBJECTIVES Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease. METHODS To achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface. FINDINGS As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism. MAIN CONCLUSION These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.
DOI: 10.6084/m9.figshare.23090470.v1
title: Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease

description: BACKGROUND Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity. OBJECTIVES Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease. METHODS To achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface. FINDINGS As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism. MAIN CONCLUSION These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.
DOI: 10.5281/zenodo.7360011
title: 3D-EdgeAngle_test-dataset

description: This dataset is meant to test 3D-EdgeAngle – A semi-automated 3D digital method to systematically quantify stone tool edge angle and design. The dataset contains a 3D model (stl format), digitalised edges (igs format) as well as the results of the edge angle calculation. The scripts for the 3D-Edge angle have been written with Python in GOM Inspect Professional 2016. The repository containing the scripts is also as available as open access on Zenodo (https://doi.org/10.5281/zenodo.7956767). The 3D model is a model of an archaeological tool (co-called <em>Keilmesser</em>) from the Upper site of Buhlen, Germany. The artefact dates to the Late Middle Palaeolithic. The archaeological finds from Buhlen are normally stored and displayed at the Hessisches Landesmuseum in Kassel, Germany (https://museum-kassel.de/de/museen-schloesser-parks/hessisches-landesmuseum). For more information about the artefact see: Schunk L. Understanding Middle Palaeolithic asymmetric stone tool design and use: functional analysis and controlled experiments to assess Neanderthal technology Verlag des Römisch- Germanischen Zentralmuseums Mainz. https://doi.org/10.11588/propylaeum.1076 The 3D model was produced with an AICON smartSCAN-HE R8 from the manufacturer Hexagon (software version OptoCat 2018R1), featuring a blue light LED and two black and white cameras with 8 megapixels each. The S-150 FOV used has a point-to-point distance of 33 µm. After scanning, the 3D model (STL file) was edited in the free software GOM Inspect 2018 (2018 Hotfix 2, Rev. 111729). The scanning took place at TraCEr, Laboratory for Traceology and Controlled Experiments at MONREPOS Archaeological Research Centre and Museum for Human Behavioural Evolution, RGZM, Neuwied, Germany.