cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/dkepf-72534
title: Saccharomyces cerevisiae - 6210

description: Raw data files of Saccharomyces cerevisiae - 6210
description: <strong>Tilt Series Date:</strong> 2017-04-21</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Markus Babst, University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight in YPD, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6.</p>
<strong>Sample Preparation:</strong> Cells were diluted to an OD of 0.2-0.25 in YPD, applied to the grid, blotted from behind manually and plunge frozen in liquid ethane-propane mix. ~200 nm lamellae were FIB milled before imaging by TEM.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-8/rawdata/</p>TS1_lamA.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-8/rawdata/TS1_lamA.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS1_4bin_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 208.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-8/3dimage_86281/TS1_4bin_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xcmw3-7vy97
title: CF83 - tcpA E183A pMT5

description: Grid box AK09Classical strain with ctxA deletion.Minor pilin tcpB knockout.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-06-18</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-18-11/rawdata/</p>20150618_AK09_10011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-18-11/rawdata/20150618_AK09_10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150618_AK09_10011_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-18-11/3dimage_58398/20150618_AK09_10011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.9knanf
title: Occurrence Download

description: A dataset containing 612 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Streptopelia chinensis",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 612 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006060-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/ast3k-ce255
title: V. cholerae JAS290 delta-tcpS

description: Classical strain with ctxA deletionStrain name JAS290 (tcpS gene knockout)Cell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2016-08-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Gabriela Kovacikova</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-36/rawdata/</p>20160807_YWC217_10036.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-36/rawdata/20160807_YWC217_10036.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160807_YWC217_10036_part121_31.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-36/3dimage_76523/20160807_YWC217_10036_part121_31.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e0wj3-zrk70
title: V. cholerae RT4236 (refreshed)

description: Grid box AK15.Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-07-13</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 N1 pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Refreshed in fresh media right before freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: C-flat Cu 2/2.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-25/rawdata/</p>20150713_AK15_10025.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-25/rawdata/20150713_AK15_10025.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150713_AK15_10025_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-13-25/3dimage_59155/20150713_AK15_10025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/znshm-g4p28
title: LCV_Legionella pneumophila

description: THP1 cells were infected with Legionella wild type cells and thereafter phagosomes (LCV) were purified by sucrose density gradient method. Phagoseomes were fixed before shipment.
description: <strong>Tilt Series Date:</strong> 2017-06-01</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Phagosome isolation method as described by Joelle Gabay (Endnote).</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-13/rawdata/</p>LCV_201706010013.mrc,  Tilt Series (Pixel Size 0.39 nm), 797.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-13/rawdata/LCV_201706010013.mrc"
        > <i class="download icon"></i> Download </a></p>

        LCV_201706010013.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-13/3dimage_84949/LCV_201706010013.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gkn6a-0ak32
title: Xanthomonas vs Caulobacter

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter competition
description: <strong>Tilt Series Date:</strong> 2017-03-09</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the strains, data collected by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-09-48/rawdata/</p>Xac_Cc_20170309a0046.mrc,  Tilt Series (Pixel Size 0.39 nm), 882.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-09-48/rawdata/Xac_Cc_20170309a0046.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_20170309a0046.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-09-48/3dimage_83089/Xac_Cc_20170309a0046.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jnak3-vdt68
title: perfusion_dcrp_fixed

description: This sample was fixed with 2.5% glutaraldehyde for 1 hour. The cells look sick, the outer membrane looks bad. Fixing for 1 hour turns out to be bad,need to fix for lesser time.
description: <strong>Tilt Series Date:</strong> 2015-08-18</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The sample was grown in perfusion chamber and fixed for 1hour with 2.5% glutaraldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1ul gold beads.</p>
<strong>Sample Preparation:</strong> The ice was too thick in the squares for which LM images were available, so could not correlate. Took tomograms of all bugs in a square.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-24/3dimage_60494/</p>perfusion_dcrp_atlas10024.rec,  Reconstruction (Pixel Size 2.4 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-24/3dimage_60494/perfusion_dcrp_atlas10024.rec"
        > <i class="download icon"></i> Download </a></p>

        perfusion_dcrp_atlas10024.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-24/rawdata/perfusion_dcrp_atlas10024.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/80a6t-bsj04
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-28/rawdata/</p>20150511_AK07_10028.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-28/rawdata/20150511_AK07_10028.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10028_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-28/3dimage_56412/20150511_AK07_10028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/86m6n-k7c38
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-10-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-104/rawdata/</p>Cb_vero_20171019b0101.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-104/rawdata/Cb_vero_20171019b0101.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_20171019b0101_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-104/3dimage_89633/Cb_vero_20171019b0101_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ycamv-r1760
title: CF83 - tcpA E183A pMT5

description: Grid box AK09Classical strain with ctxA deletion.Minor pilin tcpB knockout.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-06-18</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-18-9/rawdata/</p>20150618_AK09_10009.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-18-9/rawdata/20150618_AK09_10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150618_AK09_10009_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-06-18-9/3dimage_58394/20150618_AK09_10009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.f6v522
title: Occurrence Download

description: A dataset containing 775 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Gymnadenia orchidis"
 ]
}

 The dataset includes 775 records from 46 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006061-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/cqe95-s9f46
title: perfusion_dcrp_fixed

description: This sample was fixed with 2.5% glutaraldehyde for 1 hour. The cells look sick, the outer membrane looks bad. Fixing for 1 hour turns out to be bad,need to fix for lesser time.
description: <strong>Tilt Series Date:</strong> 2015-08-18</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The sample was grown in perfusion chamber and fixed for 1hour with 2.5% glutaraldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1ul gold beads.</p>
<strong>Sample Preparation:</strong> The ice was too thick in the squares for which LM images were available, so could not correlate. Took tomograms of all bugs in a square.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-28/3dimage_60497/</p>perfusion_dcrp_atlas10028_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
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        perfusion_dcrp_atlas10028.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
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