cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.13378958.v1
title: Supplementary Material for: Phenotype Discordance between Primary Tumor and Metastasis Impacts Metastasis Site and Outcome: Results of WSG-DETECT-PriMet

description: <b><i>Introduction:</i></b> Tumor biological factors of breast cancer (BC) such as hormone receptor (HR) status, HER2 status, and grade can differ in the metastatic cascade from primary to lymph node (LN) metastasis and to distant metastatic tissue. Systematic data regarding therapeutic consequences are yet limited. <b><i>Methods:</i></b> We conducted a prospectively planned, retrospective cohort study comparing BC phenotype in tissue from primary tumors (PTs), locoregional LN metastases, and disease recurrence (DR). HR and HER2 as well as tumor grade in PTs and DR were obtained by a database search. No centralized biomarker testing was performed. The impact of changes in tumor biological factors on post-recurrence survival (PRS) and overall survival was analyzed. <b><i>Results:</i></b> PriMet comprises 635 patients (LN tissue in 142 patients). Discrepancies for HR or HER2 status between PT and DR were observed in 18.7 and 21.6% of cases, respectively. For HR status, positivity of PT and negativity of DR was seen more often (13.2%) than vice versa (5.5%). For HER2 status, negativity of the primary and positivity of DR was seen more often (14.9%) than vice versa (6.7%). Discordance was more often observed between PT and LN metastasis compared to LN versus DR. However, numbers were small. Compared to concordant non-triple-negative (TN) disease, concordant TN disease showed significantly inferior PRS. <b><i>Conclusion:</i></b> We demonstrate receptor discordance to occur relatively frequently between PT, LN metastasis, and DR and to impact patient prognosis. However, clinical consequences of receptor discordance need to be drawn with caution considering clinical aspects as well as tumor biology.
DOI: 10.21258/1584697
title: Inventario forestale nazionale svizzero - Tabella n. 897357
DOI: 10.24381/cds.ccf781a2
title: Change in hydrological indicators for Europe from 2011 to 2095 derived from climate simulations
DOI: 10.21258/1599629
title: Inventario forestale nazionale svizzero - Tabella n. 897501
DOI: 10.21258/1569626
title: Inventaire forestier national suisse - Tableau no 897978
DOI: 10.21258/1581308
title: Inventario forestale nazionale svizzero - Tabella n. 891703
DOI: 10.6084/m9.figshare.13373771
title: test9.zip

description: Fold 9 test set
DOI: 10.6084/m9.figshare.13373771.v1
title: test9.zip

description: Fold 9 test set
DOI: 10.21258/1576662
title: Inventario forestale nazionale svizzero - Tabella n. 899633
DOI: 10.21258/1550596
title: Inventaire forestier national suisse - Tableau no 896991
DOI: 10.21258/1559196
title: Inventaire forestier national suisse - Tableau no 891906
DOI: 10.21258/1565119
title: Inventaire forestier national suisse - Tableau no 898233
DOI: 10.21258/1544415
title: Inventaire forestier national suisse - Tableau no 899185
DOI: 10.21258/1575077
title: Inventaire forestier national suisse - Tableau no 892937
DOI: 10.21253/dmu.13344836
title: COMSTAT Data - Laboratory C

description: This folder comprises confocal laser scanning microscopy COMSTAT data generated from laboratory C. These data assess 72-hour biofilm formation of <i>Pseudomonas aeruginosa</i> MPAO1 using a standardised microfluidic flow chamber and growth conditions. <br>The research was carried out as part of the Joint Programming Initiative against AntiMicrobial Resistance (JPIAMR) consortium and published in the following research article:<br><b></b>Varadarajan AR*, Allan RN*, Valentin JDP*, Castaneda Ocampo OE, Somerville V, Pietsch F, Buhmann MT, West J, Skipp PJ, van der Mei HC, Ren Q, Schreiber F, Webb JS, Aherns CH (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in <i>Pseudomonas aeruginosa</i> MPAO1. <b><i>npj Biofilms and Microbiomes</i>. </b>PMID: 33127897 DOI:<b> </b>10.1038/s41522-020-00154-8
DOI: 10.21253/dmu.13344830
title: COMSTAT Data - Laboratory B

description: This folder comprises confocal laser scanning microscopy COMSTAT data generated from laboratory B. These data assess 72-hour biofilm formation of <i>Pseudomonas aeruginosa</i> MPAO1 using a standardised microfluidic flow chamber and growth conditions. <br>The research was carried out as part of the Joint Programming Initiative against AntiMicrobial Resistance (JPIAMR) consortium and published in the following research article:<br><b></b>Varadarajan AR*, Allan RN*, Valentin JDP*, Castaneda Ocampo OE, Somerville V, Pietsch F, Buhmann MT, West J, Skipp PJ, van der Mei HC, Ren Q, Schreiber F, Webb JS, Aherns CH (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in <i>Pseudomonas aeruginosa</i> MPAO1. <b><i>npj Biofilms and Microbiomes</i>. </b>PMID: 33127897 DOI:<b> </b>10.1038/s41522-020-00154-8
DOI: 10.21253/dmu.13344812
title: COMSTAT Data - Laboratory A

description: This folder comprises confocal laser scanning microscopy COMSTAT data generated from laboratory A. These data assess 72-hour biofilm formation of <i>Pseudomonas aeruginosa</i> MPAO1 using a standardised microfluidic flow chamber and growth conditions. <br>The research was carried out as part of the Joint Programming Initiative against AntiMicrobial Resistance (JPIAMR) consortium and published in the following research article:<br><b> </b>Varadarajan AR*, Allan RN*, Valentin JDP*, Castaneda Ocampo OE, Somerville V, Pietsch F, Buhmann MT, West J, Skipp PJ, van der Mei HC, Ren Q, Schreiber F, Webb JS, Aherns CH (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in <i>Pseudomonas aeruginosa</i> MPAO1. <b><i>npj Biofilms and Microbiomes</i>. </b>PMID: 33127897 DOI:<b> </b>10.1038/s41522-020-00154-8
DOI: 10.21258/1554300
title: Inventaire forestier national suisse - Tableau no 892899
DOI: 10.21258/1566600
title: Inventaire forestier national suisse - Tableau no 898766
DOI: 10.21253/dmu.13344836.v1
title: COMSTAT Data - Laboratory C

description: This folder comprises confocal laser scanning microscopy COMSTAT data generated from laboratory C. These data assess 72-hour biofilm formation of <i>Pseudomonas aeruginosa</i> MPAO1 using a standardised microfluidic flow chamber and growth conditions. <br>The research was carried out as part of the Joint Programming Initiative against AntiMicrobial Resistance (JPIAMR) consortium and published in the following research article:<br><b></b>Varadarajan AR*, Allan RN*, Valentin JDP*, Castaneda Ocampo OE, Somerville V, Pietsch F, Buhmann MT, West J, Skipp PJ, van der Mei HC, Ren Q, Schreiber F, Webb JS, Aherns CH (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in <i>Pseudomonas aeruginosa</i> MPAO1. <b><i>npj Biofilms and Microbiomes</i>. </b>PMID: 33127897 DOI:<b> </b>10.1038/s41522-020-00154-8
DOI: 10.21253/dmu.13344830.v1
title: COMSTAT Data - Laboratory B

description: This folder comprises confocal laser scanning microscopy COMSTAT data generated from laboratory B. These data assess 72-hour biofilm formation of <i>Pseudomonas aeruginosa</i> MPAO1 using a standardised microfluidic flow chamber and growth conditions. <br>The research was carried out as part of the Joint Programming Initiative against AntiMicrobial Resistance (JPIAMR) consortium and published in the following research article:<br><b></b>Varadarajan AR*, Allan RN*, Valentin JDP*, Castaneda Ocampo OE, Somerville V, Pietsch F, Buhmann MT, West J, Skipp PJ, van der Mei HC, Ren Q, Schreiber F, Webb JS, Aherns CH (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in <i>Pseudomonas aeruginosa</i> MPAO1. <b><i>npj Biofilms and Microbiomes</i>. </b>PMID: 33127897 DOI:<b> </b>10.1038/s41522-020-00154-8
DOI: 10.21253/dmu.13344812.v1
title: COMSTAT Data - Laboratory A

description: This folder comprises confocal laser scanning microscopy COMSTAT data generated from laboratory A. These data assess 72-hour biofilm formation of <i>Pseudomonas aeruginosa</i> MPAO1 using a standardised microfluidic flow chamber and growth conditions. <br>The research was carried out as part of the Joint Programming Initiative against AntiMicrobial Resistance (JPIAMR) consortium and published in the following research article:<br><b> </b>Varadarajan AR*, Allan RN*, Valentin JDP*, Castaneda Ocampo OE, Somerville V, Pietsch F, Buhmann MT, West J, Skipp PJ, van der Mei HC, Ren Q, Schreiber F, Webb JS, Aherns CH (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in <i>Pseudomonas aeruginosa</i> MPAO1. <b><i>npj Biofilms and Microbiomes</i>. </b>PMID: 33127897 DOI:<b> </b>10.1038/s41522-020-00154-8
DOI: 10.21258/1530359
title: Inventaire forestier national suisse - Tableau no 898035
DOI: 10.21258/1564881
title: Inventaire forestier national suisse - Tableau no 897046
DOI: 10.17866/rd.salford.8226251.v1
title: Research Timeline

description: Research timeline<br>
DOI: 10.21258/1621325
title: Inventario forestale nazionale svizzero - Tabella n. 898780
DOI: 10.21258/1595610
title: Inventario forestale nazionale svizzero - Tabella n. 897316
DOI: 10.21258/1596479
title: Inventario forestale nazionale svizzero - Tabella n. 899053
DOI: 10.25919/jekk-e341
title: Genotype by Sequencing FASTQ data for Spotted Handfish

description: Demultiplexed FASTQ files from GBS analysis at AGRF for Spotted Handfish (Brachionichthys hirsutus) from River Derwent. GBS undertaken for genetic diversity, gene flow and connectivity assessment of Spotted Handfish. Raw and filtered variant call files from the GBS analysis provided. Strata metadata for spotted handfish individuals from the Derwent estuary provided. GenePop input file for eight 2006-2008 spotted handfish collections from the Derwent estuary provided.
DOI: 10.21258/1625229
title: Inventario forestale nazionale svizzero - Tabella n. 898226
DOI: 10.21258/1535454
title: Inventaire forestier national suisse - Tableau no 897633
DOI: 10.21258/1540348
title: Inventaire forestier national suisse - Tableau no 902236
DOI: 10.21258/1579357
title: Inventario forestale nazionale svizzero - Tabella n. 897982
DOI: 10.5517/ccdc.csd.cc21q9gk
title: CCDC 1898268: Experimental Crystal Structure Determination

description: Related Article: Si Wu, Lei Mei, Kong-Qiu Hu, Zhi-Fang Chai, Chang-Ming Nie, Wei-Qun Shi|2019|Wuji Cailiao Xuebao|35|118|doi:10.15541/jim20190118
DOI: 10.5517/ccdc.csd.cc24hbgg
title: CCDC 1980945: Experimental Crystal Structure Determination

description: Related Article: Weichi Liang, Jiali Wen, Yukai Zhao, Meng Zhang, Jiuxing Jiang|2020|Dalton Trans.|49|17033|doi:10.1039/D0DT03006F
DOI: 10.5517/ccdc.csd.cc25x0kp
title: CCDC 2022922: Experimental Crystal Structure Determination

description: Related Article: Weichi Liang, Jiali Wen, Yukai Zhao, Meng Zhang, Jiuxing Jiang|2020|Dalton Trans.|49|17033|doi:10.1039/D0DT03006F
DOI: 10.5517/ccdc.csd.cc2609j2
title: CCDC 2026083: Experimental Crystal Structure Determination

description: Related Article: Ulrich Schubert, Rene Kogler, Christine Artner|2020|CSD Communication|||
DOI: 10.5517/ccdc.csd.cc260s9b
title: CCDC 2026541: Experimental Crystal Structure Determination

description: Related Article: Yüksel Sahin, Armin Berndt, Gertraud Geiseler, Werner Massa|2020|CSD Communication|||
DOI: 10.5517/ccdc.csd.cc2611lx
title: CCDC 2026798: Experimental Crystal Structure Determination

description: Related Article: Zhang Heyi|2020|CSD Communication|||
DOI: 10.5517/ccdc.csd.cc266p15
title: CCDC 2032206: Experimental Crystal Structure Determination

description: Related Article: Abhijit Garai, Kumar Biradha|2020|Cryst.Growth Des.|20|8059|doi:10.1021/acs.cgd.0c01305
DOI: 10.5517/ccdc.csd.cc266p26
title: CCDC 2032207: Experimental Crystal Structure Determination

description: Related Article: Abhijit Garai, Kumar Biradha|2020|Cryst.Growth Des.|20|8059|doi:10.1021/acs.cgd.0c01305
DOI: 10.5517/ccdc.csd.cc266p37
title: CCDC 2032208: Experimental Crystal Structure Determination

description: Related Article: Abhijit Garai, Kumar Biradha|2020|Cryst.Growth Des.|20|8059|doi:10.1021/acs.cgd.0c01305
DOI: 10.5517/ccdc.csd.cc266p48
title: CCDC 2032209: Experimental Crystal Structure Determination

description: Related Article: Abhijit Garai, Kumar Biradha|2020|Cryst.Growth Des.|20|8059|doi:10.1021/acs.cgd.0c01305
DOI: 10.5517/ccdc.csd.cc266p59
title: CCDC 2032210: Experimental Crystal Structure Determination

description: Related Article: Abhijit Garai, Kumar Biradha|2020|Cryst.Growth Des.|20|8059|doi:10.1021/acs.cgd.0c01305
DOI: 10.5517/ccdc.csd.cc24t19b
title: CCDC 1990271: Experimental Crystal Structure Determination

description: Related Article: Soumen Ghosh, Mehebub Ali Khan, Arghyadeep Bhattacharyya, Md. Akhtarul Alam, Ennio Zangrando, Nikhil Guchhait|2020|New J.Chem.|44|20275|doi:10.1039/C9NJ06184C
DOI: 10.5517/ccdc.csd.cc209ry1
title: CCDC 1856433: Experimental Crystal Structure Determination

description: Related Article: Soumen Ghosh, Mehebub Ali Khan, Arghyadeep Bhattacharyya, Md. Akhtarul Alam, Ennio Zangrando, Nikhil Guchhait|2020|New J.Chem.|44|20275|doi:10.1039/C9NJ06184C
DOI: 10.5517/ccdc.csd.cc209s15
title: CCDC 1856436: Experimental Crystal Structure Determination

description: Related Article: Soumen Ghosh, Mehebub Ali Khan, Arghyadeep Bhattacharyya, Md. Akhtarul Alam, Ennio Zangrando, Nikhil Guchhait|2020|New J.Chem.|44|20275|doi:10.1039/C9NJ06184C
DOI: 10.5517/ccdc.csd.cc26f1xm
title: CCDC 2038340: Experimental Crystal Structure Determination

description: Related Article: Soumen Ghosh, Mehebub Ali Khan, Arghyadeep Bhattacharyya, Md. Akhtarul Alam, Ennio Zangrando, Nikhil Guchhait|2020|New J.Chem.|44|20275|doi:10.1039/C9NJ06184C
DOI: 10.5517/ccdc.csd.cc252tnr
title: CCDC 1998714: Experimental Crystal Structure Determination

description: Related Article: Maykon Alves Lemes, José Antônio do Nascimento Neto, Freddy Fernandes Guimarães, Lauro June Queiroz Maia, Ricardo Costa de Santana, Felipe Terra Martins|2020|New J.Chem.|44|20259|doi:10.1039/D0NJ04558F
DOI: 10.5517/ccdc.csd.cc252tqt
title: CCDC 1998716: Experimental Crystal Structure Determination

description: Related Article: Maykon Alves Lemes, José Antônio do Nascimento Neto, Freddy Fernandes Guimarães, Lauro June Queiroz Maia, Ricardo Costa de Santana, Felipe Terra Martins|2020|New J.Chem.|44|20259|doi:10.1039/D0NJ04558F
DOI: 10.5517/ccdc.csd.cc252vbg
title: CCDC 1998735: Experimental Crystal Structure Determination

description: Related Article: Shao-Liang Zhang, Shan-Shan Li, Xin-Chao Li, Xian-Hui Hou, Zhi-Cheng Wang, Xiao-Ying Ji, Lei Chen|2020|New J.Chem.|44|19996|doi:10.1039/D0NJ04763E
DOI: 10.5517/ccdc.csd.cc26lwb1
title: CCDC 2043933: Experimental Crystal Structure Determination

description: Related Article: A. Budanow, H.-W. Lerner, M. Bolte|2020|Acta Crystallogr.,Sect.E:Cryst.Commun.|76|1837|doi:10.1107/S2056989020015066
DOI: 10.5517/ccdc.csd.cc26lwc2
title: CCDC 2043934: Experimental Crystal Structure Determination

description: Related Article: A. Budanow, H.-W. Lerner, M. Bolte|2020|Acta Crystallogr.,Sect.E:Cryst.Commun.|76|1837|doi:10.1107/S2056989020015066
DOI: 10.5517/ccdc.csd.cc26m602
title: CCDC 2044233: Experimental Crystal Structure Determination

description: Related Article: A. Jochim, R. Radulovic, I. Jess, C. Näther|2020|Acta Crystallogr.,Sect.E:Cryst.Commun.|76|1841|doi:10.1107/S2056989020015121
DOI: 10.21258/1591035
title: Inventario forestale nazionale svizzero - Tabella n. 899315
DOI: 10.15139/s3/vszz6m
title: Vision Zero Boulder: Safe Streets Report (2019). Boulder, Colorado

description: Vision Zero is a strategy to reduce all traffic fatalities and severe injuries to zero, while increasing safe, equitable, and healthy mobility for all. Vision Zero Plans help guide municipalities, counties, and other areas to address these strategies considering the local context.
DOI: 10.21258/1623659
title: Inventario forestale nazionale svizzero - Tabella n. 891971
DOI: 10.6084/m9.figshare.13379006
title: Supplementary Material for: Coronary Computed Tomography Angiography in Diagnosing Obstructive Coronary Artery Disease in Patients with Advanced Chronic Kidney Disease: A Systematic Review and Meta-Analysis

description: <b><i>Introduction:</i></b> Coronary computed tomography angiography (CCTA) is emerging as an important noninvasive testing modality for coronary angiography. The performance characteristic of CCTA in patients with advanced kidney disease is unknown. <b><i>Methods:</i></b> We performed a systematic review and meta-analysis of studies specifically investigating the sensitivity and specificity of CCTA compared to coronary angiogram as a reference standard in patients with advanced kidney disease, defined as dialysis dependence or nearing kidney transplantation. Two independent investigators assessed studies for inclusion/exclusion, quality, and characteristics, while a third investigator adjudicated. <b><i>Results:</i></b> We identified 4 studies including a total of 217 patients, of whom 159 were dialysis dependent. Three of the 4 studies had a high risk of bias in patient selection and study flow, while 1 study rated low in all areas of bias. The studies were heterogeneous in their patient selection and CCTA protocol but consistent in their definition of obstructive coronary artery disease. The pooled sensitivity and specificity for CCTA were 0.96 (0.87–0.99) and 0.66 (0.57–0.74), respectively. When we restricted the analysis to dialysis-dependent patients, the pooled sensitivity and specificity for CCTA were 0.99 (0.74–1.00) and 0.67 (0.49–0.82), respectively. <b><i>Conclusions:</i></b> Based on limited data, CCTA appears to have comparable sensitivity but lower specificity relative to the non-kidney disease population.
DOI: 10.21258/1545868
title: Inventaire forestier national suisse - Tableau no 898868
DOI: 10.21258/1533154
title: Inventaire forestier national suisse - Tableau no 899136
DOI: 10.5281/zenodo.4278991
title: Data from: Schneider et al. (2020). Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa. Taxon.

description: DNA sequence alignments with all loci concatenated (e.g., "Alignment_concatenated_xxx_dataset") or for each locus separated (see folders "Gene_alignments_xxx_dataset"). The individual gene alignments are identified by their locus number. Numbers in the sequence headers of the fasta files correspond to the Lab IDs of specimens (see related publication for detailed voucher information). These alignments were used for the phylogenetic analyses in the related publication. The bait set contains the probe sequences used for the targeted enrichment of nuclear loci of Ochnaceae.
DOI: 10.5281/zenodo.4278990
title: Data from: Schneider et al. (2020). Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa. Taxon.

description: DNA sequence alignments with all loci concatenated (e.g., "Alignment_concatenated_xxx_dataset") or for each locus separated (see folders "Gene_alignments_xxx_dataset"). The individual gene alignments are identified by their locus number. Numbers in the sequence headers of the fasta files correspond to the Lab IDs of specimens (see related publication for detailed voucher information). These alignments were used for the phylogenetic analyses in the related publication. The bait set contains the probe sequences used for the targeted enrichment of nuclear loci of Ochnaceae.
DOI: 10.21258/1580949
title: Inventario forestale nazionale svizzero - Tabella n. 891906
DOI: 10.21258/1572905
title: Inventaire forestier national suisse - Tableau no 898491
DOI: 10.34740/kaggle/dsv/1746243
title: CryoNuSeg
DOI: 10.21258/1531197
title: Inventaire forestier national suisse - Tableau no 897095
DOI: 10.5519/qd.zzo5hb9r
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (0 records)
DOI: 10.21258/1536755
title: Inventaire forestier national suisse - Tableau no 896993
DOI: 10.21258/1540456
title: Inventaire forestier national suisse - Tableau no 902162
DOI: 10.5281/zenodo.4322674
title: Hypertrophic Cardiomyopathy. Insights from Genetic Analysis and Cardiopulmonary Exercise Testing

description: Raw data of the paper "Hypertrophic Cardiomyopathy. Insights from Genetic Analysis and Cardiopulmonary Exercise Testing"
DOI: 10.5281/zenodo.4322673
title: Hypertrophic Cardiomyopathy. Insights from Genetic Analysis and Cardiopulmonary Exercise Testing

description: Raw data of the paper "Hypertrophic Cardiomyopathy. Insights from Genetic Analysis and Cardiopulmonary Exercise Testing"
DOI: 10.21258/1577668
title: Inventario forestale nazionale svizzero - Tabella n. 897221
DOI: 10.21258/1586340
title: Inventario forestale nazionale svizzero - Tabella n. 898832
DOI: 10.21258/1624137
title: Inventario forestale nazionale svizzero - Tabella n. 896960
DOI: 10.21258/1539830
title: Inventaire forestier national suisse - Tableau no 892893
DOI: 10.21258/1544202
title: Inventaire forestier national suisse - Tableau no 891737
DOI: 10.21258/1555929
title: Inventaire forestier national suisse - Tableau no 897528
DOI: 10.21258/1618404
title: Inventario forestale nazionale svizzero - Tabella n. 897409
DOI: 10.5281/zenodo.4322801
title: Sodium hexacyanoferrate(II) decahydrate

description: Sodium hexacyanoferrate(II) decahydrate
DOI: 10.5281/zenodo.4322800
title: Sodium hexacyanoferrate(II) decahydrate

description: Sodium hexacyanoferrate(II) decahydrate
DOI: 10.21258/1566443
title: Inventaire forestier national suisse - Tableau no 898200
DOI: 10.21258/1617254
title: Inventario forestale nazionale svizzero - Tabella n. 897909
DOI: 10.21258/1615289
title: Inventario forestale nazionale svizzero - Tabella n. 896989
DOI: 10.21258/1606974
title: Inventario forestale nazionale svizzero - Tabella n. 899646
DOI: 10.21258/1574096
title: Inventaire forestier national suisse - Tableau no 896917
DOI: 10.21258/1607156
title: Inventario forestale nazionale svizzero - Tabella n. 898566
DOI: 10.21258/1621085
title: Inventario forestale nazionale svizzero - Tabella n. 899192
DOI: 10.21258/1556800
title: Inventaire forestier national suisse - Tableau no 897909
DOI: 10.5281/zenodo.4322792
title: input_tibble

description: decoupleR Benchmark Vignette Input tibble
DOI: 10.21258/1597943
title: Inventario forestale nazionale svizzero - Tabella n. 898237
DOI: 10.21258/1621505
title: Inventario forestale nazionale svizzero - Tabella n. 899327
DOI: 10.6084/m9.figshare.12643031
title: Gene expression raw count by RNA sequence

description: Two maize inbred lines with contrasting salt tolerance were used in the present study, of which L2010-3 is a salt-tolerant line and BML1234 is a salt-sensitive line. The seeds from the two lines were surface-sterilized in 10% (v/v) H2O2 for 15 min, rinsed with distilled water and then germinated on filter paper saturated with distilled water. Uniform seedlings with two leaves were transplanted into aerated 1 × Hoagland’s nutrient solution + Na150 for salt stress . The seedlings were then cultured in a growth room with a relative humidity of 70% (14/10 h, day/night) at 26℃. At 0 h (control), 6 h (T-6), 18 h (T-18), and 36 h (T-36) under salt treatment, the mixed roots from three seedlings of each line were individually collected as the samples for transcriptome sequencing, with two biological replicates.<br>According to the manufacturer’s instructions, total RNA of each sample was isolated using the HiPure Plant RNA Maxi Kit purchased from Magen company (http://www.magentec.com.cn). RNA library construction and transcriptome sequencing were performed by the Illumina sequencing platform.<br>Raw sequencing reads were filtered to remove low-quality reads, adaptor-polluted reads, and reads containing Poly-N, which generated clean reads by fastp (Chen et al. 2018). The clean reads were subsequently mapped to the maize reference genome (ZmB73 RefGen_V4) (http://www.gramene.org) via Hisat2. Gene expression levels were determined by Htseq software and were normalized to transcripts per million (TPM) by consumer script.