DOI: 10.5061/dryad.9cnp5hqzw
title: Data from: Prevalence and consequences of heterospecific pollen receipt in the subalpine plant communities of <em>Polemonium brandegeei</em>

description: Many pollinators visit multiple plant species, causing pollinators to
 deposit diverse pollen loads. Although heterospecific pollen (HP) transfer
 is widespread, few studies have tested how HP deposition affects plant
 fitness in natural populations. We investigated the fitness consequences
 of HP receipt within two floral communities surrounding Polemonium
 brandegeei (Polemoniaceae), a subalpine herb with diverse pollinators.
 First, we collected open pollinated stigmas from abundant species in
 flower (n = 11) and generated pollen transfer networks. All sampled
 species received or donated HP with distantly related species. Positive
 relations between HP receipt and conspecific pollen (CP) number and
 germination indicated facilitation due to pollinator sharing in P.
 brandegeei and Harbouria trachypleura (Apiaceae); however, HP receipt was
 low and did not affect CP germination in most species. Second, we
 conducted hand pollinations to test how donor-recipient relatedness
 affected consequences of HP receipt for P. brandegeei. A distantly related
 donor did not affect CP germination, seed set, or seed weight, but two
 congeneric donors reduced reproductive measures. This study suggests that
 pollinator sharing between native co-flowering species is common and can
 be beneficial in plant communities. However, competition may occur if
 closely related species begin to co-flower due to range changes or
 phenological shifts.
description: README file for: pollenTrasferNetwork_CO-DM.csv
 pollenTrasferNetwork_WY-VD.csv Data from pollen loads observed on stigmas
 of four (CO-DM) and eight (WY-VD) species: pollen counts and
 identification. The "species" column identifies the species the
 stigma belongs to. Species codes are described in Table S1 and below. The
 "stigmaID" column identifies the unique stigma code. The
 "germinatedCP" column identifies the number of germinated
 conspecific pollen grains observed on the stigma. The "CP"
 column identifies the total amount of conspecific pollen grains observed
 on the stigma. The "HP" column identifies the total amount of
 heterospecific pollen grains observed on the stigma. The remaining columns
 identify the amount of heterospecific pollen grains of a specific species
 observed on the stigma. Species codes are described in Table S1 and below.
 Species codes are as follows: *Antennaria anaphaloides* (Aa)
 *Arctostaphylus uva-ursi* (Au) *Cerastium arvense* (Ca) *Drymocallis
 fissa* (Df) *Erigeron pumilus* (Ep) *Harbouria trachypleura* (Ht)
 Indeterminate (In) Large indeterminate (Lin) *Maiathemum stellatum* (Ms)
 *Mertensia lanceolata* (Ml) *Microseris nutans* (Mn) *Oxytropis sericea*
 (Os) *Penstemon rydbergii* (Pr) *Pinus* spp. (P) *Polemonium brandegeei*
 (Pb) *Ranunculus ranunculinus* (Rr) *Ribes cereum* (Rc) *Rubus deliciosus*
 (Rd) *Senecio integerrimus* (Si) Small indeterminate (Sin) Textured
 indeterminate (Tin) *Thermopsis divaricapra* (Td) README file for:
 hpDonor-RecipientRelatedness.csv Data from hand pollination experiments on
 10 plants at CO-DM (Deer Mountain, Colorado), 10 plants at WY-VD (Vedauwoo
 Recreation Area, Wyoming), and 21 plants at GH (Crop Technology Centre
 Greenhouse, University of Manitoba). The "plantID" column
 identifies the unique plant individual code. The "location"
 column identifies where the individual was located and where hand
 pollinations took place: CO-DM, WY-VD, or GH. The "treatment"
 column identifies what pollen was applied to the stigma: CP (100 %
 conspecific pollen), Mix.Pp (~ 50 % conspecific pollen:50 % Polemonium
 pulcherrimum pollen), Mix.Td (~ 50 % conspecific pollen:50 % *Thermopsis
 divaricarpa* pollen), or Mix.Pc (~ 50 % conspecific pollen:50 %
 *Polemonium caeruleum* pollen). The "seedNumber" column
 identifies the number of seeds produced by the gynoecium the pollen
 treatment was applied to. The "seedWeight" column identifies the
 total weight of the seeds produced by the gynoecium the pollen treatment
 was applied to. The "germinatedCP" column identifies the number
 of germinated conspecific pollen grains observed on the stigma the
 treatment was applied to.