cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.12772715
title: Increasing Recombinant Protein Production in E. coli via FACS-Based Selection of N-terminal Coding DNA Libraries

description: Data for a manuscript to be deposited to the FEBS Journal. 

Abstract as deposited:

We present a novel approach to modify N-terminal sequences of recombinant proteins to increase their production yield in Escherichia coli. Prior research has demonstrated a profound impact of a few nucleotides following the initiator codon (further to as N-terminal sequences) on their expression. Most of these investigations have been limited to selecting from a few rationally designed sequences. In contrast we used directed evolution-based methodology screening large numbers of diversified sequences derived from DNA libraries coding for the N-termini of investigated proteins. To facilitate the identification of cells with increasing expression of the target construct, we cloned a GFP gene at the C-terminus of the expressed genes and used fluorescent activated cell sorting (FACS) to separate cells based on their fluorescence. By following this systematic workflow, we successfully elevated the yield of soluble recombinant proteins of multiple constructs up to over 30-fold.
DOI: 10.5281/zenodo.12772716
title: Increasing Recombinant Protein Production in E. coli via FACS-Based Selection of N-terminal Coding DNA Libraries

description: Data for a manuscript to be deposited to the FEBS Journal. 

Abstract as deposited:

We present a novel approach to modify N-terminal sequences of recombinant proteins to increase their production yield in Escherichia coli. Prior research has demonstrated a profound impact of a few nucleotides following the initiator codon (further to as N-terminal sequences) on their expression. Most of these investigations have been limited to selecting from a few rationally designed sequences. In contrast we used directed evolution-based methodology screening large numbers of diversified sequences derived from DNA libraries coding for the N-termini of investigated proteins. To facilitate the identification of cells with increasing expression of the target construct, we cloned a GFP gene at the C-terminus of the expressed genes and used fluorescent activated cell sorting (FACS) to separate cells based on their fluorescence. By following this systematic workflow, we successfully elevated the yield of soluble recombinant proteins of multiple constructs up to over 30-fold.
DOI: 10.15468/dl.kc2yfz
title: Occurrence Download

description: A dataset containing 88 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-8.08692 37.04892,-7.99495 36.94841,-7.79165 36.92326,-7.59722 37.06303,-7.57045 37.10262,-7.47218 37.16329,-7.59199 37.18613,-7.77286 37.09905,-7.87394 37.11312,-8.08692 37.04892))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is one of (Spartina maritima (Curtis) Fernald, Zostera noltii Hornem.)",
 "Year 1970-2024"
 ]
}

 The dataset includes 88 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028885-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.pv287r
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Boana buriti (Caramaschi & Cruz, 1999)"
 ]
}

 The dataset includes 14 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028886-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.7910/dvn/ylzvjc/726ocd
title: SEM_BEL 118 1000.tif

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DOI: 10.7910/dvn/0pd4jm/cafr0b
title: rivm_corona_in_nl_fatalities.tab
DOI: 10.7910/dvn/0pd4jm/dnx3vg
title: rivm_corona_in_nl_hosp.tab
DOI: 10.7910/dvn/0pd4jm/i5lnyz
title: rivm_corona_in_nl_daily.tab
DOI: 10.7910/dvn/0pd4jm/lqnlnf
title: nl-covid-19_31032020.tar.gz

description: 31 March 2020
DOI: 10.7910/dvn/0pd4jm/x41kzm
title: nl-covid-19_31072020.tar.gz

description: July 31, 2020
DOI: 10.7910/dvn/1solng/6zt9oa
title: queen2_dT10ms_00466.dat

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DOI: 10.7910/dvn/1solng/8p9htk
title: queen2_dT10ms_00465.dat

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DOI: 10.7910/dvn/1solng/f2ztig
title: queen2_dT10ms_00461.dat

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DOI: 10.7910/dvn/1solng/ku48q6
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DOI: 10.7910/dvn/1solng/yqetut
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DOI: 10.7910/dvn/gpffaq/5c22aj
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description: July 31, 2020
DOI: 10.7910/dvn/gpffaq/f4cb2j
title: spain-covid-19_30052020.tar.gz

description: May 30, 2020
DOI: 10.7910/dvn/gpffaq/jchbco
title: spain-covid-19_31032020.tar.gz

description: 31 March 2020
DOI: 10.7910/dvn/gpffaq/krelxv
title: spain-covid-19_30072020.tar.gz

description: July 30, 2020
DOI: 10.7910/dvn/gpffaq/mm5eqn
title: spain-covid-19_30062020.tar.gz

description: June 30, 2020
DOI: 10.7910/dvn/ylzvjc/6okdgl
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DOI: 10.7910/dvn/ylzvjc/cropxk
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DOI: 10.7910/dvn/ylzvjc/oeyawl
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DOI: 10.7910/dvn/ylzvjc/sril3j
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DOI: 10.7910/dvn/ylzvjc/zwikil
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DOI: 10.7910/dvn/0pd4jm/9bd791
title: rivm_corona_in_nl.tab
DOI: 10.7910/dvn/0pd4jm/cjuiqn
title: rivm_corona_in_nl_table.tab
DOI: 10.7910/dvn/1solng/0xuqpq
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DOI: 10.7910/dvn/1solng/3k1rfl
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DOI: 10.7910/dvn/1solng/vhvp6y
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DOI: 10.7910/dvn/1solng/fbsfe0
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DOI: 10.7910/dvn/1solng/7hkdkj
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DOI: 10.7910/dvn/1solng/jyc8sx
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title: queen2_dT10ms_00479.dat

description: :unav
DOI: 10.7910/dvn/4lvary/13culi
title: fiscal capacity_overview conjoint.pdf

description: overview of the two experiments
DOI: 10.7910/dvn/ue7lb5/gjt6c2
title: GG_DG_script_final_final-0210-1.edat2

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title: GG_DG_script_final_final-0111-1.edat2

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DOI: 10.7910/dvn/ue7lb5/rgct7z
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DOI: 10.7910/dvn/ue7lb5/tzmfrs
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DOI: 10.7910/dvn/1solng/6dwa6e
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DOI: 10.7910/dvn/1solng/rbh6x1
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DOI: 10.7910/dvn/1solng/xhcz0q
title: queen2_dT10ms_00489.dat

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DOI: 10.7910/dvn/4lvary/oy5x0d
title: hypothesis file.tab

description: This file provides an overview of the registered hypotheses
DOI: 10.7910/dvn/ue7lb5/9eu5uj
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DOI: 10.7910/dvn/srhrdt/ejv60p
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DOI: 10.7910/dvn/ue7lb5/6kgdb2
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