cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/h1hdr-0tp35
title: Rhesus Macaque Fibroblast

description: Proteasome inhibition Campbell et al 2008 Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate to any virus because light image only taken in the red channel. Discovered the autofluorescent problem when looking at fibroblasts with no virus, bright puncta everywhere and correlate to onions
description: <strong>Tilt Series Date:</strong> 2015-05-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr-Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift. Proteasome inhibition 200mg/ml</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-4/3dimage_57244/</p>tomography13.rec,  Reconstruction (Pixel Size 24.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-4/3dimage_57244/tomography13.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography13.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-4/rawdata/tomography13.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography13_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-4/3dimage_76016/tomography13_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h21vs-4m315
title: MEF cells - Dynamin2 knockdown

description: Dynamin2 knocked down with retroviral delivery of shRNA to study mitochondrial division. grid info - DG2_grid1.
description: <strong>Tilt Series Date:</strong> 2018-11-27</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun Shik from Chan lab helped design shRNA and provided protocols for viral transduction into Chan lab's MEF cells. Shrawan and Danielle made the cell line. Shrawan FIB-milled and performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin (10min)</p>
<strong>Sample Preparation:</strong> Cells were treated with 10 µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-17/rawdata/</p>TS9.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-17/rawdata/TS9.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS10-pt49.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-17/rawdata/TS10-pt49.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS9_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-17/3dimage_102050/TS9_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a40ft-n5873
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-79/3dimage_73331/</p>20150511_AK07_10079_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-79/3dimage_73331/20150511_AK07_10079_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10079.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-79/rawdata/20150511_AK07_10079.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10079_SIRT_2K.rec~,  5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-79/file_16632/20150511_AK07_10079_SIRT_2K.rec~"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gcyx8-f7694
title: lighlyfixed_wt_serumbottle

description: Raw data files of lighlyfixed_wt_serumbottle
description: <strong>Tilt Series Date:</strong> 2015-07-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Sahand and Moh USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown using serum bottle. This sample was lightly fixed with 2.5% glutaraldehyde, the very same sample that was sent for SEM. After mixing with glutaraldehyde in a tube, the sample was applied on grid and frozen.</p>
<strong>Sample Preparation:</strong> blotting conditions: 4 ul sample, blot force 6, blot time 3, drain time 1s</p>
  psa2015-07-06-6_slicer16556.jpg: nucleotide??
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-6/rawdata/</p>atlas1_fixedwt_serumbottle0006.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-6/rawdata/atlas1_fixedwt_serumbottle0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas1_fixedwt_serumbottle0006_part121_6.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-06-6/3dimage_58683/atlas1_fixedwt_serumbottle0006_part121_6.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2015-07-06-6_slicer16560.png,  testing grab n note.  please ignore, 583.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2015-07-06-6_slicer16560.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v7jx2-49p61
title: S. cerevisiae - 6210 + pAM21

description: Raw data files of S. cerevisiae - 6210 + pAM21
description: <strong>Tilt Series Date:</strong> 2016-10-07</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Babst Lab (Markus Babst and Akshay Moharir) - Provided strain with the plasmid transformed.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 6210 + pAM21(Vps36-V5-Strep in Ura plasmid). Grown to saturation overnight and cut back to 0.2 OD and grown until 0.6 OD. Media -> -URA YNB</p>
<strong>Sample Preparation:</strong> Resuspended in PBS and manually blotted from behind at 0.1 OD or blotted using Vitrobot at 0.6 OD directly in growth medium.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-07-5/3dimage_80807/</p>TS2-NS_D.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-07-5/3dimage_80807/TS2-NS_D.rec"
        > <i class="download icon"></i> Download </a></p>

        TS2-NS_D.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-07-5/rawdata/TS2-NS_D.mrc"
        > <i class="download icon"></i> Download </a></p>

        LAMD_TS2-NS_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 252.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-07-5/3dimage_81442/LAMD_TS2-NS_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.z4cub2
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus sideroxylon",
 "Country is Tunisia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045046-250811113504898/datasets/export for details.
DOI: 10.22002/59bpt-emq69
title: Saccharomyces cerevisiae - JAWY1

description: Raw data files of Saccharomyces cerevisiae - JAWY1
description: <strong>Tilt Series Date:</strong> 2016-10-31</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-10-31-4to5 (TS3,TS4)- Heat shocked @38 deg C for 2min10min. Other sm2016-10-31-* - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.</p>
<strong>Sample Preparation:</strong> Final OD of 0.2 on grids (in growth medium) and blotted from behind</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-4/3dimage_80816/</p>TS3_part121_6.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-4/3dimage_80816/TS3_part121_6.rec"
        > <i class="download icon"></i> Download </a></p>

        TS3.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-4/rawdata/TS3.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS3_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 215.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2016-10-31-4/3dimage_81451/TS3_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0qdk5-mfs56
title: M. xanthus knockout minor pilins

description: Grid box YWC130Strain SA6815
description: <strong>Tilt Series Date:</strong> 2015-04-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-6/3dimage_54848/</p>20150426_YWC130_10006_part121_1.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-6/3dimage_54848/20150426_YWC130_10006_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>

        20150426_YWC130_10012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-6/rawdata/20150426_YWC130_10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150426_YWC130_10012_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-6/3dimage_54863/20150426_YWC130_10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8fq29-fst86
title: HeLa cells expressing TRIM5α-YFP

description: HeLa cell line 25 expressing stable TRIM5α-YFP. Correlated lightelectron microscopy (11062015Tom). Concentration of 500 nmblue beads (phosphorex) 4/150. 20 nm gold highly concentrated.Manual blotting with Whatman 40.HeLa cell line 25 expressingstable TRIM5α-YFP.
description: <strong>Tilt Series Date:</strong> 2015-10-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cell line 25 and VSV-G pseudotyped virus Vpr-Tomato provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells infected with pseudovirus Vpr-Tomato (in box). 500 µl of media and 500 µl of virus. Incubated with MG-132 (Campbell et al 2008) 6hrs. Cells were washed with warm media and incubated at 37°C before freezing.</p>
<strong>Sample Preparation:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-8/3dimage_71021/</p>tomography19.rec,  Reconstruction (Pixel Size 24.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-8/3dimage_71021/tomography19.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography19.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-8/rawdata/tomography19.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography19_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-8/3dimage_76109/tomography19_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.wcwfp6
title: Occurrence Download

description: A dataset containing 57 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus sideroxylon",
 "Country is South Africa",
 "HasCoordinate is true"
 ]
}

 The dataset includes 57 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045045-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/66jzz-tp034
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-10/rawdata/</p>20160424_YWC212_10012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-10/rawdata/20160424_YWC212_10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_10012_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-10/3dimage_74839/20160424_YWC212_10012_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_10012_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-10/3dimage_74840/20160424_YWC212_10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.bnyqgr
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Trochalopteron melanostigma",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 14 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045047-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.