cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/8a7-4ea-h9k
title: Pseudofusus rostratus (Olivi, 1792)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ynx-7ya-xxt
title: Solatia piscatoria (Gmelin, 1791)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/5v5-tw7-1ny
title: Mitridae Swainson, 1831

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yng-s44-b6g
title: Gibberula subtrigona (P. P. Carpenter, 1864)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/xxk3stz8h9
title: data set pmi

description: Retrospective dataset of geriatric patients (≥65 years) diagnosed with pneumonia, including demographic data, clinical parameters, laboratory results, pectoral muscle index (PMI) measurements, and calculated risk scores (SII, PNI, SOAR). Data were used to evaluate the predictive contribution of PMI to 30-day mortality in combination with established scoring systems.
DOI: 10.3535/wg8-pxb-xh6
title: Latirus Montfort, 1810

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/etg-1l0-ye5
title: Indothais gradata (Jonas, 1846)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/me7-hxv-k5j
title: Odostomella doliolum (R. A. Philippi, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/xxk3stz8h9.1
title: data set pmi

description: Retrospective dataset of geriatric patients (≥65 years) diagnosed with pneumonia, including demographic data, clinical parameters, laboratory results, pectoral muscle index (PMI) measurements, and calculated risk scores (SII, PNI, SOAR). Data were used to evaluate the predictive contribution of PMI to 30-day mortality in combination with established scoring systems.
DOI: 10.3535/kw1-f0b-n60
title: Nucella lapillus (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yxj-0b6-w2t
title: Relegamoria molleri Iredale, 1936

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ppx-fjz-hbg
title: Gibberula epigrus (Reeve, 1865)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yst-eqq-yl1
title: Murex occa G. B. Sowerby II, 1834

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/191-rkd-eay
title: Buccinidae Rafinesque, 1815

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/29e-93d-vdh
title: Drilliola loprestiana (Calcara, 1841)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/dat-82y-bx3
title: Gastropoda Cuvier, 1795

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hlb-cc4-n57
title: Melo amphora ([Lightfoot, 1786])

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hqt-dj2-4xm
title: Parthenina emaciata (Brusina, 1866)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/v9w-h27-car
title: Rhizorus Montfort, 1810

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/cg3-7g0-tld
title: Oenopota declivis (Lovén, 1846)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/e6b-v7h-sma
title: Retusidae Thiele, 1925

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/3f8-nfb-ccl
title: Pseudonebularia rubritincta (Reeve, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gyd-64p-28m
title: Turris crispa (Lamarck, 1816)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/rjy-qqc-9dj
title: Acteon tornatilis (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/cbndpwhw73
title: Frey et al Western Blot Images

description: Original Western Blot images relevant to publication "Targeting an essential viral oncoprotein with an IL-7-enhanced mRNA vaccine induces durable immunity to Merkel cell carcinoma."
DOI: 10.3535/2fs-1qy-e1r
title: Buccinidae Rafinesque, 1815

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/h4n-8k3-xsg
title: Buccinidae Rafinesque, 1815

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/c45-xq2-v3x
title: Chrysallida P. P. Carpenter, 1856

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/tcb-f7t-mj7
title: Drupella rugosa (Born, 1778)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/cbndpwhw73.1
title: Frey et al Western Blot Images

description: Original Western Blot images relevant to publication "Targeting an essential viral oncoprotein with an IL-7-enhanced mRNA vaccine induces durable immunity to Merkel cell carcinoma."
DOI: 10.3535/8ws-28k-q26
title: Tricolia nordsiecki (Talavera, 1978)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gfx-nqf-erv
title: Mitridae Swainson, 1831

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/m5p-xq8-rzt
title: Tritia reticulata (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/e0g-pl7-hme
title: Parthenina flexuosa (Monterosato, 1874)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vxx-wx1-xxk
title: Ringicula propinquans Hinds, 1844

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/zen-17f-d6g
title: Idyla bicristata (Rossmässler, 1839)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.6084/m9.figshare.29922665
title: Data used in the paper: General Patterns and Predictors of Thermal Tolerance in Bees: A Synthesis Across Taxa and Environments

description: Data used in the paper: General Patterns and Predictors of Thermal Tolerance in Bees: A Synthesis Across Taxa and Environments
DOI: 10.6084/m9.figshare.29922665.v1
title: Data used in the paper: General Patterns and Predictors of Thermal Tolerance in Bees: A Synthesis Across Taxa and Environments

description: Data used in the paper: General Patterns and Predictors of Thermal Tolerance in Bees: A Synthesis Across Taxa and Environments
DOI: 10.3535/msf-hek-cm7
title: Mitromorpha olivoidea (Cantraine, 1835)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/pkz-nhk-l7l
title: Persicula cingulata (Dillwyn, 1817)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9qm-mgl-ryx
title: Pyramidellidae J. E. Gray, 1840

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/psj-q02-9e1
title: Granulina Jousseaume, 1888

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ybd-eta-2bn
title: Retusidae Thiele, 1925

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/zl4-kpe-xsr
title: Tritia reticulata (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hh4-sjm-8z9
title: Conus patricius Hinds, 1843

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/ys34fgz4cx.2
title: ARID1A stabilises non-homologous end joining factors at DNA breaks induced by the G4 ligand pyridostatin. Sousa, Amin, et al.

description: Raw data for Sousa, Amin, et al., 2025
Abstract:
ARID1A is a subunit of the BAF chromatin remodelling complex that is frequently mutated in cancer. It is challenging to predict how ARID1A loss impacts cancer therapy response because it participates in many different cellular pathways. G quadruplex (G4) binding ligands, such as pyridostatin, have shown anticancer effects, but the pathways and genetic determinants involved in the response to G4 ligands are still not fully understood. Here, we show that ARID1A deficient cells are selectively sensitive to pyridostatin when compared with isogenic controls. Sensitivity to pyridostatin was apparent in ovarian and colorectal cancer cell line models, and in vivo studies suggest that G4 ligands hold promise for treating ARID1A deficient cancers. While we find that ARID1A impacts on pyridostatin-induced transcriptional responses, we find that pyridostatin-mediated toxicity in ARID1A-deficient cells is driven by defective DNA repair of topoisomerase-induced breaks. We show that ARID1A-deficient cells are unable to efficiently accumulate non-homologous end joining proteins on chromatin following pyridostatin exposure. These data uncover a role for ARID1A in the cellular response to G4 ligands, and link remodelling to G4 ligand-induced transcriptional and DNA damage responses.
DOI: 10.3535/j2v-h3a-tks
title: Philine iris Tringali, 2001

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/p23-kjk-1g7
title: Marginellidae J. Fleming, 1828

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/a4f-5xh-7tr
title: Conus insularis Gmelin, 1791

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/mdh-233-cx9
title: Pyrgiscus rufus (R. A. Philippi, 1836)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0n1-dsf-316
title: Scaphander lignarius (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gtx-1zg-xs9
title: Tjaernoeia exquisita (Jeffreys, 1883)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/mqp-4td-d5g
title: Scaphella junonia (Lamarck, 1804)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/skd-j43-x3a
title: Tritia reticulata (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/dcr-5lj-4ps
title: Sinistrofulgur perversum (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jx0-qz2-7yw
title: Conus boeticus Reeve, 1844

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/6n7-lgy-rnw
title: Ringicula conformis Monterosato, 1877

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/shx-ymk-395
title: Kelletia kelletii (Forbes, 1852)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/kk8-td9-c0q
title: Pseudomelatoma moesta (P. P. Carpenter, 1864)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yn5-s4p-wkj
title: Acteocina knockeri (E. A. Smith, 1872)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ad0-e0b-9dx
title: Pyrgocythara stosiciana (Brusina, 1869)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gw2-f8x-sgh
title: Megastomia conoidea (Brocchi, 1814)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/1xz-698-jt8
title: Conus chaldaeus (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9d2-kac-w9k
title: Haminoeidae Pilsbry, 1895

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/hdp8njjrdn.1
title: A small-scale demonstration tank for real-time wave energy control: Tiny-T

description: This dataset accompanies the paper "A Small-Scale Demonstration Tank for Real-Time Wave Energy Control", which introduces the Tiny-T platform—a compact, low-cost, and portable wave tank developed to demonstrate wave energy control strategies in real time. It contains all design files necessary to build and operate the Tiny-T, organised to support both replication of the tank construction and reproduction of the experimental tests described in the paper.
DOI: 10.3535/1vm-q2k-96m
title: Conus biliosus (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/a5l-twv-pas
title: Turridae H. Adams & A. Adams, 1853 (1838)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/twj-jx5-bg6
title: Conus coronatus Gmelin, 1791

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/eag-9tp-c0c
title: Ringicula conformis Monterosato, 1877

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/r3w-0bw-n8w
title: Skeneopsis planorbis (O. Fabricius, 1780)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/sy6-btf-ac7
title: Buccinidae Rafinesque, 1815

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/w2z-xnc-tv2
title: Pugilina morio (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gre-q9y-l89
title: Usilla tosana (Pilsbry, 1904)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qqj-3s7-5y2
title: Oliva caerulea (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/r35-rjg-td4
title: Vullietoliva splendidula (G. B. Sowerby I, 1825)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/j92gxxthv4.6
title: Ground composition data for the southernmost area of Lérida province, NE Spain

description: More than 3000 physical, chemical and morphological data of soils described between December 1980 and July 1981 in the southernmost part of the province of Lérida, NE Spain, are collected in this dataset. The soils are located between the coordinates 41.62°N 1.06°E, and 41.28°N 0.34°E.
Most of the soil samples were taken in backhoe dug pits where their profiles were described. The data —presented here in an Excel spreadsheet— come from the book by J. Porta & R. Julià (eds.) published in 1982 with I.S.B.N. 84-5008771-6. Moreover, two chapters were scanned from this book and included in this dataset as “Description.pdf” and “FormationClassification.pdf”. The author did not proofread the galley proofs of these chapters. The data contained in “Description.pdf” were manually transcribed in 2025 to the excel file “ProfilesLerida04clas.xlsx”.
Care must be taken with the location coordinates given the non-correction of the galley proofs and because the resources available to us 45 years ago were quite rudimentary. The excel file “ProfilesLerida04clas.xlsx” incorporates the correction of obvious errors in the coordinates and their transformation to ETRS89 from the notations used in 1980-81. The help of Ms. Rosa Gómez-Báguena with the coordinates is greatly appreciated.
We also present the file "1989 XVI Reun.SECS Lérida suelos secano", a report including discussion and data about the studied soils.

This research was possible thanks to the grant PID2021-127170OB-I00 funded by
MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, and the grant
TED2021-130303B-I00 funded by MCIN/AEI/10.13039/501100011033 and by the “European Union NextGeneration EU/PRTR”.
DOI: 10.3535/c1r-0mc-rz8
title: Conus chytreus Tryon, 1884

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qdd-3vp-tp4
title: Buccinidae Rafinesque, 1815

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/7v7-780-xb7
title: Conus virgo Linnaeus, 1758

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/hr9rs5gtng
title: Data - Stress, anxiety, depression and cardiovascular risk factors in female emergency nursing professionals

description: This is a research database of female nursing professionals working in emergency services. The database is structured with socio-occupational variables, emotional state (stress, anxiety, depression), and cardiovascular risk factors.
DOI: 10.3535/0de-x3d-1lb
title: Scaphander gracilis R. B. Watson, 1883

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/kdr-k2x-p6m
title: Tritia varicosa (W. Turton, 1825)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/t3f-rda-wra
title: Turridae H. Adams & A. Adams, 1853 (1838)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vjp-tyl-4te
title: Conus sponsalis Hwass, 1792

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/hr9rs5gtng.2
title: Data - Stress, anxiety, depression and cardiovascular risk factors in female emergency nursing professionals

description: This is a research database of female nursing professionals working in emergency services. The database is structured with socio-occupational variables, emotional state (stress, anxiety, depression), and cardiovascular risk factors.
DOI: 10.3535/83f-qa7-522
title: Conus coronatus Gmelin, 1791

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fq7-d7d-zdd
title: Euthria cornea (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/v34-xfb-jgs
title: Tibersyrnola unifasciata (Forbes, 1844)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/rne-eqx-xx7
title: Peristernia carotiana (Tapparone Canefri, 1881)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s5g-xq4-vaq
title: Prodotia iostoma (J. E. Gray, 1833)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0ll-9zd-p2x
title: Tritia mutabilis (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hk1-kk3-l2d
title: Turrilatirus craticulatus (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/sdk-wb5-d8a
title: Tritia miga (Bruguière, 1789)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.17632/g9fd55k94c.1
title: Arithmetic fact retrieval deficits in chronic stroke – a deficit of relearning?

description: This dataset contains processed lesion data of patients in the chronic phase of stroke (&gt; 6 months) with initial Aphasia. They were tested on Addition, Subtraction and Multiplication, but only Multiplication was analysed using Lesion Behaviour Mapping. See "how to reproduce" for more details on the analysis. Results suggest that a multiplication deficit in the chronic phase of stroke stems from a deficit of arithmetic re-learning, as lesion behaviour mapping indicated mostly parahippocampal areas involved in (re-)learning.
DOI: 10.3535/kzg-s78-whf
title: Mitridae Swainson, 1831

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/j2d-6vk-wh9
title: Tridacna crocea Lamarck, 1819

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/41t-y1l-g04
title: Marshallora adversa (Montagu, 1803)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/s7d-mw9-8n9
title: Conus striolatus Kiener, 1848

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9j4-4sc-6lv
title: Ringicula conformis Monterosato, 1877

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/qhe-h59-t5w
title: Tiberia pulchella (A. Adams, 1854)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vpt-pdf-bl4
title: Conus guinaicus Hwass, 1792

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.6084/m9.figshare.28030676
title: Lysine-specific histone demethylase complex restricts Epstein-Barr virus lytic reactivation

description: Epstein-Barr virus (EBV) infects &gt;95% of adults and contributes to several human cancers. EBV can remain latent where viral lytic genes are silenced, precluding use of antiviral agents like ganciclovir. Little is known about host factors involved in EBV latency. Here, we performed a human genome-wide CRISPR-Cas9 screen in Burkitt lymphoma B cells, which identified lysine-specific histone demethylase 1 (LSD1) and its co-repressors REST Corepressor 1 (CoREST) and zinc finger protein 217 (ZNF217) as critical for EBV latency. Gene knockout or LSD1 inhibition triggered EBV reactivation, and the latter sensitized cells to ganciclovir cytotoxicity, including in murine tumor xenografts. Mechanistically, ZNF217 recruits LSD1 and CoREST to form a complex that binds a specific DNA motif associated with regions implicated in EBV reactivation. It removes histone 3 lysine 4 (H3K4) methylation marks and restricts host DNA looping. Alternatively, the H3K4 lysine methyltransferase 2D supports EBV lytic reactivation. Our results highlight H3K4 methylation as a major EBV lytic switch regulator and therapeutic target.
DOI: 10.6084/m9.figshare.28030676.v1
title: Lysine-specific histone demethylase complex restricts Epstein-Barr virus lytic reactivation

description: Epstein-Barr virus (EBV) infects &gt;95% of adults and contributes to several human cancers. EBV can remain latent where viral lytic genes are silenced, precluding use of antiviral agents like ganciclovir. Little is known about host factors involved in EBV latency. Here, we performed a human genome-wide CRISPR-Cas9 screen in Burkitt lymphoma B cells, which identified lysine-specific histone demethylase 1 (LSD1) and its co-repressors REST Corepressor 1 (CoREST) and zinc finger protein 217 (ZNF217) as critical for EBV latency. Gene knockout or LSD1 inhibition triggered EBV reactivation, and the latter sensitized cells to ganciclovir cytotoxicity, including in murine tumor xenografts. Mechanistically, ZNF217 recruits LSD1 and CoREST to form a complex that binds a specific DNA motif associated with regions implicated in EBV reactivation. It removes histone 3 lysine 4 (H3K4) methylation marks and restricts host DNA looping. Alternatively, the H3K4 lysine methyltransferase 2D supports EBV lytic reactivation. Our results highlight H3K4 methylation as a major EBV lytic switch regulator and therapeutic target.