DOI: 10.5061/dryad.s4mw6m9g0
title: Data from: Stomatal patterning is differently regulated in adaxial and abaxial epidermis in Arabidopsis

description: Stomatal pores in leaves mediate CO2 uptake into the plant and water loss
 via transpiration. Most plants are hypostomatous with stomata present only
 in the lower leaf surface (abaxial epidermis). Many herbs, including the
 model plant Arabidopsis thaliana, have substantial numbers of stomata also
 on the upper (adaxial) leaf surface. Studies of stomatal development have
 mostly focused on abaxial stomata and very little is known of adaxial
 stomatal formation. We addressed the role of leaf number in determination
 of stomatal density and stomatal ratio, and studied adaxial and abaxial
 stomatal patterns in mutants deficient in known abaxial stomatal
 development regulators. We found that stomatal density in some genetic
 backgrounds varies between different fully expanded leaves and recommend
 using defined leaves for analyses of stomatal patterning. Our results
 indicate that stomatal development is at least partly independently
 regulated in adaxial and abaxial epidermis, as i) plants deficient in ABA
 biosynthesis and perception have increased stomatal ratios, ii) the
 epf1epf2, tmm and sdd1 mutants have reduced stomatal ratios, iii) erl2
 mutants have increased adaxial but not abaxial stomatal index, and iv)
 stomatal precursors preferentially occur in abaxial epidermis. Further
 studies of adaxial stomata can reveal new insights into stomatal form and
 function.
description: # Data from: Stomatal patterning is differently regulated in adaxial and
 abaxial epidermis in Arabidopsis
 [https://doi.org/10.5061/dryad.s4mw6m9g0](https://doi.org/10.5061/dryad.s4mw6m9g0) ## Description of the data and file structure The data are from two experiments: First experiment: The data includes adaxial and abaxial stomatal density and stomatal ratio determined on leaves 6 and 8 of 7-week-old Arabidopsis (*Arabidopsis thaliana*) plants and on leaves 10 and 12 of 8-week-old Arabidopsis plants via dental silicone and nail varnish impressions and brightfield microscopy. Second experiment: The data includes adaxial and abaxial stomatal density, size and index, total stomatal density from leaf 8 of 6-week-old Arabidopsis plants collected via dental silicone and nail varnish impressions and brightfield microscopy. In addition, projected rosette area was determined from the same plants a 4 weeks of age. ### Files and variables #### File: Supplementary\_Data.xlsx **Description:** Data accompanying preprint [https://doi.org/10.1101/2024.02.22.581564](https://doi.org/10.1101/2024.02.22.581564) and article published in Journal of Experimental Botany ([https://doi.org/10.1093/jxb/erae354](https://doi.org/10.1093/jxb/erae354)) ##### Variables * Sheet 'Figure 2': data from experiment 1, L6 to L12 denote respective leaves, plant line denotes respective Arabidopsis genotypes, analysed data are shown in figure 2 of the preprint and manuscript; variables include stomatal density and stomatal ratio * Sheet 'Figure 3': data from experiment 2, L8 denotes leaf 8, plant line denotes respective Arabidopsis genotypes, analysed data are shown in figure 3 of the preprint and manuscript, variables include stomatal density and stomatal index * Sheet 'Figure 4': data from experiment 2, L8 denotes leaf 8, plant line denotes respective Arabidopsis genotypes, analysed data are shown in figure 4 of the preprint and manuscript, variables include stomatal length * Sheet 'Figure 6': data from experiment 2, L8 denotes leaf 8, plant line denotes respective Arabidopsis genotypes, analysed data are shown in figure 6 of the preprint and manuscript, variables include total stomatal density and projected rosette area ## Access information Other publicly accessible locations of the data: * [https://www.biorxiv.org/content/biorxiv/early/2024/07/19/2024.02.22.581564/DC3/embed/media-3.xlsx?download=true](https://www.biorxiv.org/content/biorxiv/early/2024/07/19/2024.02.22.581564/DC3/embed/media-3.xlsx?download=true)