DOI: 10.5061/dryad.sj3tx963w
title: Microsatellite data of Vincetoxicum hirundinaria offspring and their inferred mother plants from 13 populations in the South-Western Finnish Archipelago

description: Fragmented landscapes may have implications for the genetic structure of
 populations and for the microevolution of plant species. In particular,
 landscape fragmentation and/or population isolation might affect the
 evolution of plant mating systems. Here, we study the consequences of
 landscape fragmentation on the genetic structure of populations of a
 perennial herb, Vincetoxicum hirundinaria with a mixed mating system. Our
 study area, the south-western Finnish archipelago, was formed after the
 glacial ice sheet started to retreat 12 000 years ago. Due to the
 isostatic land uplift following the glacial retreat, suitable habitats
 have been formed gradually, and as a consequence, populations of V.
 hirundinaria differ in age, size and their degree of isolation in the
 area. We hypothesized that a mixed-mating system has been selected for in
 these populations due to the advantage of self-fertilization in newly
 colonized areas and the advantage of outcrossing in adaptation to
 heterogeneous environments. To test this hypothesis, we collected seeds of
 open-pollinated flowers from 13 V. hirundinaria populations differing in
 size, age and isolation, and used 15 microsatellite markers to perform
 progeny-array analysis to estimate population-level outcrossing rates,
 population genetic indices and population structure. We found that V.
 hirundinaria is almost completely outcrossing in the study area with no
 signs of past self-fertilization and/or mating among relatives. The
 overall low inbreeding coefficients indicate that even in small
 populations mating among relatives is rare. High allelic richness of both
 maternal and offspring genotypes as well as limited genetic
 differentiation among the studied populations indicate strong gene flow
 among them. Our findings suggest that V. hirundinaria has successful seed
 and pollen dispersal among populations that has allowed colonization of
 new habitats in this fragmented landscape and led to a genetically
 well-mixed group of populations at the scale of the study.
description: The data file
 Muola_offspring_genotypes.txt contains allele sizes of 13 microsatellite
 loci across all successfully analysed Vincetoxicum hirundinaria offspring
 sampled from mother plants from 13 populations in the South-Western
 Finnish Archipelago. For the laboratory methods, see the main paper and
 the associated Supporting information S1. The data file contains the data
 after quality check using INEST and MLTR, i.e. after removal of specific
 seed families, of complete loci and of loci for specific seed families
 (see the main paper, first paragraph of the Methods section “Mating system
 analysis”), leaving 1255 samples. The data file
 Muola_mother_genotypes.txt contains the genotypes of 153 mother plants
 inferred from the offspring genotypes in the data file
 Muola_offspring_genotypes.txt.
description: Both data files are
 tab-delimited. The first column indicates the offspring or mother ID
 respectively. The second column shows abbreviated population names. The
 remaining 26 columns contain the microsatellite allele lengths in
 basepairs for 13 microsatellite loci. Each pair of columns bears the same
 microsatellite locus name for the two alleles. Missing values are
 indicated with “NA”.