cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14318177.v1
title: Antidiabetic activity screening and nmr profile of vegetable and spices commonly consumed in Indonesia

description: Abstract Apart from their nutritional value, vegetables and spices were empirically known to have functional properties such as antioxidant and antidiabetic activity. However, scientific evidence of these health effects is limited. In our effort to find potent raw material for antidiabetic functional food development, α-glucosidase inhibition and antioxidant activity of 15 Indonesian vegetables and spices methanolic extracts were screened. Their phytochemical profiles were assessed by determining total phenolic content (TPC) and 1H NMR fingerprinting. The results showed that the Syzygium polyanthum, Pluchea indica, Etlingera elaitor, and Cosmos caudatus had the highest GIA (IC50 11.76 ± 0.32, 12.17 ± 0.18, 53.13 ± 2.87, and 61.33 ± 1.21 μg/ml, respectively). The AA and TPC of the four samples were also higher than the others. The 1H NMR profiles of the active samples were different from the non-active samples mainly in the aromatic region. Further observation of the spectra revealed that caffeoylquinic derivatives and esculetin were identified in P. indica; while gallic acid, syringic acid, and myricetin were identified in S. polyanthum. These compounds were known to have antidiabetic activity through different mechanisms.
DOI: 10.6084/m9.figshare.14318180
title: Shelf-life of loricariid catfish (Pterygoplichthys disjunctivus [Weber, 1991]) roe stored in ice

description: Abstract Loricariid catfish (Pterygoplichthys disjunctivus [Weber, 1991]) has invaded water catchments throughout the world, due to the lack of predators and high fecundity. An approach to control its population could be using its roe as food; however, more knowledge is needed. Thus, the present study aimed to evaluate the shelf-life of fresh loricariid catfish roe stored in ice, as an approach for possible food utilisation. Physicochemical characteristics (moisture content, aw, pH, hardness, and colour), protein electrophoresis (SDS-PAGE), amino and fatty acid contents, oxidative stability (TBARS) and microbiology of roe were analysed at 0, 2, 4, 6, 11, 15 and 21 days. Moisture content (58.5 ± 2.1 vs. 60.4 ± 1.2%), aw (0.89) and pH (5.87 ± 0.14 vs. 5.96 ± 0.03) were shelf-stable (initial vs. final day, respectively). SDS-PAGE showed minor hydrolysis of its protein. Ice storage increased roe free amino acid concentration, confirming proteolysis of its proteins/peptides with not impact on the fatty acid content and TBARS (2.4–3.3 mg malonadehyde/kg sample). Thus, ice storage of fresh loricariid catfish (P. disjunctivus [Weber, 1991]) roe, under the study conditions, proved to be an effective preservation method for its food utilisation, maintaining its physicochemical and microbiological characteristics for up to 20 days.
DOI: 10.6084/m9.figshare.14318180.v1
title: Shelf-life of loricariid catfish (Pterygoplichthys disjunctivus [Weber, 1991]) roe stored in ice

description: Abstract Loricariid catfish (Pterygoplichthys disjunctivus [Weber, 1991]) has invaded water catchments throughout the world, due to the lack of predators and high fecundity. An approach to control its population could be using its roe as food; however, more knowledge is needed. Thus, the present study aimed to evaluate the shelf-life of fresh loricariid catfish roe stored in ice, as an approach for possible food utilisation. Physicochemical characteristics (moisture content, aw, pH, hardness, and colour), protein electrophoresis (SDS-PAGE), amino and fatty acid contents, oxidative stability (TBARS) and microbiology of roe were analysed at 0, 2, 4, 6, 11, 15 and 21 days. Moisture content (58.5 ± 2.1 vs. 60.4 ± 1.2%), aw (0.89) and pH (5.87 ± 0.14 vs. 5.96 ± 0.03) were shelf-stable (initial vs. final day, respectively). SDS-PAGE showed minor hydrolysis of its protein. Ice storage increased roe free amino acid concentration, confirming proteolysis of its proteins/peptides with not impact on the fatty acid content and TBARS (2.4–3.3 mg malonadehyde/kg sample). Thus, ice storage of fresh loricariid catfish (P. disjunctivus [Weber, 1991]) roe, under the study conditions, proved to be an effective preservation method for its food utilisation, maintaining its physicochemical and microbiological characteristics for up to 20 days.
DOI: 10.6084/m9.figshare.14318185.v1
title: Variability in the shelf life of table grapes from same batch when exposed under different ambient air conditions

description: Abstract This study aimed to evaluate the effect of the variability in ambient air conditions (temperature and relative humidity) and periods of exposure on the same batch of table grapes in the first stages of the fruit supply chain (harvesting, processing and storage prior to commercialization), in order to predict their dynamic shelf life. To estimate the shelf life we used a linear prediction model dependent on time and ambient air conditions (temperature and relative humidity). It was noted that during processing, there was considerable variations in ambient air conditions that grapes were exposed to, especially in harvesting stage. The ambient of following stages showed homogeneity in air conditions, but the residence time and the way of palletizing grapes affected their shelf life. We observed heterogeneity in the shelf life among different grape pallets and among boxes on a same pallet, with variations of up to 34 days. Thus, we ascertained a heterogeneity in the quality of table grapes from the same production batch, due to the variability in ambient air conditions and period of exposure, which should be considered in the logistics management of the dynamic shelf life of the product in the cold chain.
DOI: 10.6084/m9.figshare.14318185
title: Variability in the shelf life of table grapes from same batch when exposed under different ambient air conditions

description: Abstract This study aimed to evaluate the effect of the variability in ambient air conditions (temperature and relative humidity) and periods of exposure on the same batch of table grapes in the first stages of the fruit supply chain (harvesting, processing and storage prior to commercialization), in order to predict their dynamic shelf life. To estimate the shelf life we used a linear prediction model dependent on time and ambient air conditions (temperature and relative humidity). It was noted that during processing, there was considerable variations in ambient air conditions that grapes were exposed to, especially in harvesting stage. The ambient of following stages showed homogeneity in air conditions, but the residence time and the way of palletizing grapes affected their shelf life. We observed heterogeneity in the shelf life among different grape pallets and among boxes on a same pallet, with variations of up to 34 days. Thus, we ascertained a heterogeneity in the quality of table grapes from the same production batch, due to the variability in ambient air conditions and period of exposure, which should be considered in the logistics management of the dynamic shelf life of the product in the cold chain.
DOI: 10.6084/m9.figshare.14318188
title: Counting and identification of molds and yeasts in dry salted shrimp commercialized in Rio Branco, Acre, Brazil

description: Abstract Dry salted shrimp is a popular food in Acre cuisine, especially as a main ingredient in “tacacá” and “rabada no tucupi”. However, different intrinsic and extrinsic factors can result in accelerated deterioration and microbial proliferation, causing food poisoning to the consumer. The objective was to perform the counting and identification of molds and yeasts in dry salted shrimp in establishments that sell typical foods in the municipality of Rio Branco, state of Acre. Shrimp samples from six establishments were collected in sterile bottles, identified, refrigerated and transported to the Laboratory of Infectious Diseases of Animals at the Federal University of Acre, for the manual counting of colony-forming units and identification of fungal genera. As a result, the number of molds and yeasts in the dried salted shrimp samples varied between 1.0x102 and 1.02x106 CFU/g. Nine fungal genera were identified: Aspergillus, Penicillium, Cladosporium, Malassezia, Fusarium, Exophiala, Candida, Curvularia and Trichosporon. The most frequent were Aspergillus, Penicillium and Cladosporium, these being potential producers of mycotoxins. The presence of molds and yeasts in the samples of dried salted shrimp shows the need for greater hygienic-sanitary rigidity of this fish at different stages of manipulation and processing as preventative measures for public health.
DOI: 10.6084/m9.figshare.14318189.v1
title: Development and characterization of two novel formulations of Labneh cheese of sheep’s milk

description: Abstract The growth of dairy sheep farming in Brazil combined with consumer´s preference for concentrated yogurts and the search for innovative products with different flavors and textures demonstrate the opportunity for diversification of sheep milk derivatives. The objective of this study was to develop two novel formulations of Labneh cheese made of sheep’s milk and to verify their shelf life considering microbiological and physical-chemical quality under refrigeration storage. Formulations L1 and L2 were prepared using a similar protocol with pressing times of 4 and 3 hours respectively, and this difference caused little influence on the parameters evaluated, showing no need for this additional time. Both formulations had the desired spreadability characteristic and the final products showed moisture and fat content classified as very high moisture and semi-fat cheeses according to the Brazilian legislation. The microbiological counts in L1 and L2 after 150 days of cold storage were within legal limits for thermotolerant coliforms, Staphylococcus aureus, Salmonella sp. and Listeria monocytogenes. The satisfactory results in the microbiological and physical-chemical evaluations demonstrated that the Labneh formulations and processing method were efficient to produce a stable product of good quality, indicating that it can be commercialized to a specific niche market.
DOI: 10.6084/m9.figshare.14318189
title: Development and characterization of two novel formulations of Labneh cheese of sheep’s milk

description: Abstract The growth of dairy sheep farming in Brazil combined with consumer´s preference for concentrated yogurts and the search for innovative products with different flavors and textures demonstrate the opportunity for diversification of sheep milk derivatives. The objective of this study was to develop two novel formulations of Labneh cheese made of sheep’s milk and to verify their shelf life considering microbiological and physical-chemical quality under refrigeration storage. Formulations L1 and L2 were prepared using a similar protocol with pressing times of 4 and 3 hours respectively, and this difference caused little influence on the parameters evaluated, showing no need for this additional time. Both formulations had the desired spreadability characteristic and the final products showed moisture and fat content classified as very high moisture and semi-fat cheeses according to the Brazilian legislation. The microbiological counts in L1 and L2 after 150 days of cold storage were within legal limits for thermotolerant coliforms, Staphylococcus aureus, Salmonella sp. and Listeria monocytogenes. The satisfactory results in the microbiological and physical-chemical evaluations demonstrated that the Labneh formulations and processing method were efficient to produce a stable product of good quality, indicating that it can be commercialized to a specific niche market.
DOI: 10.6084/m9.figshare.14318191
title: Aflatoxin M1: biological decontamination methods in milk and cheese

description: Abstract Dairy cattle when fed on aflatoxin B1 may excrete aflatoxin M1 in milk as a consequence of dietary exposure. Once AFM1 is excreted in milk, it is present in dairy products such as cheese, yogurt, among others. This mycotoxin is quite resistant to temperature therefore heat treatments like pasteurization and ultra-pasteurization are not enough to inactivate it. In this context, this article provides an overview of the biological decontamination methods on milk and cheese of the last decade, as a contribution to evaluate the evolution of this strategy as well as its efficiency according to their authors. Relevant studies published between January 2009 and May 2019 were selected after a systematic search of the literature in PubMed, Science direct and Google Scholar databases. According with our research in the last decade few studies have been published on these methods and unfortunately none of the published studies tested such methods in cheese. Throughout the research many studies on decontamination methods were found, however, in phosphate buffered saline solution or in culture medium. Further studies on biological decontamination on milk and mainly on cheese are necessary for this technique to be better developed and applied to a large scale in the industries.
DOI: 10.6084/m9.figshare.14318191.v1
title: Aflatoxin M1: biological decontamination methods in milk and cheese

description: Abstract Dairy cattle when fed on aflatoxin B1 may excrete aflatoxin M1 in milk as a consequence of dietary exposure. Once AFM1 is excreted in milk, it is present in dairy products such as cheese, yogurt, among others. This mycotoxin is quite resistant to temperature therefore heat treatments like pasteurization and ultra-pasteurization are not enough to inactivate it. In this context, this article provides an overview of the biological decontamination methods on milk and cheese of the last decade, as a contribution to evaluate the evolution of this strategy as well as its efficiency according to their authors. Relevant studies published between January 2009 and May 2019 were selected after a systematic search of the literature in PubMed, Science direct and Google Scholar databases. According with our research in the last decade few studies have been published on these methods and unfortunately none of the published studies tested such methods in cheese. Throughout the research many studies on decontamination methods were found, however, in phosphate buffered saline solution or in culture medium. Further studies on biological decontamination on milk and mainly on cheese are necessary for this technique to be better developed and applied to a large scale in the industries.
DOI: 10.6084/m9.figshare.14318192.v1
title: Antioxidant and anti-diabetic properties of Spirulina platensis produced in Turkey

description: Graphical Abstract : TOC-Table of content. Abstract Spirulina produced in Turkey has a high antioxidant capacity determined by the three common methods (ABTS, CUPRAC, and DPPH). The major phenolics found in Spirulina were acacetin (53.62%) and pinocembrin (41.28%). The bio-accessibility values of the phenolic compounds in Spirulina were approximately 60%. PUFA’s are the significant antioxidant compounds to prevent lipid peroxidation. Spirulina has been found to reduce blood sugar and oxidative stress due probably to the high amount of ω-6 PUFA. By the treatment of Spirulina, the levels of antioxidant enzymes (GSH-Px and SOD) were increased 240 and 60% in the healthy rats, while 19 and 59% in the diabetics. In diabetic rats fed with Spirulina, glucose, triglyceride, total cholesterol levels in blood and malondialdehyde content in body tissues were decreased by 20, 31, 22 and up to 56%, respectively. In-vitro and in-vivo tests have shown that Spirulina has anti-hyperglycaemic, anti-hyperlipidaemia and antioxidative effects on diabetic rats.
DOI: 10.6084/m9.figshare.14318192
title: Antioxidant and anti-diabetic properties of Spirulina platensis produced in Turkey

description: Graphical Abstract : TOC-Table of content. Abstract Spirulina produced in Turkey has a high antioxidant capacity determined by the three common methods (ABTS, CUPRAC, and DPPH). The major phenolics found in Spirulina were acacetin (53.62%) and pinocembrin (41.28%). The bio-accessibility values of the phenolic compounds in Spirulina were approximately 60%. PUFA’s are the significant antioxidant compounds to prevent lipid peroxidation. Spirulina has been found to reduce blood sugar and oxidative stress due probably to the high amount of ω-6 PUFA. By the treatment of Spirulina, the levels of antioxidant enzymes (GSH-Px and SOD) were increased 240 and 60% in the healthy rats, while 19 and 59% in the diabetics. In diabetic rats fed with Spirulina, glucose, triglyceride, total cholesterol levels in blood and malondialdehyde content in body tissues were decreased by 20, 31, 22 and up to 56%, respectively. In-vitro and in-vivo tests have shown that Spirulina has anti-hyperglycaemic, anti-hyperlipidaemia and antioxidative effects on diabetic rats.
DOI: 10.6084/m9.figshare.14318194
title: Ultrafiltration for the recovery of proteins from surimi washing water

description: Abstract The aim of this study was to evaluate the efficiency of the ultrafiltration process (UF) in the cleansing treatment of the tilapia surimi washing water and in the recovery of components. After producing surimi, the water generated in the washing operations was subjected to UF in a 30 kDa polyethersulfone membrane. Unfiltered washing waters and UF permeate and concentrate were analyzed for pH, total solids (TS) and fixed solids (FS), total proteins, and lipids. The chemical oxygen demand (COD) was determined in the original water and in the permeate. The concentrate was dehydrated in a spray dryer and analyzed for amino acids and carotenoids. The permeate showed a significant decrease in TS, proteins and COD, indicating that UF was efficient in removing the organic load from the waters. The percentage of protein recovery by UF was 93.12% and the dehydrated concentrate had all the essential amino acids, being leucine (5.47%), lysine (6.49%), valine (3.59%), and phenylalanine (3.68%) those with the highest concentrations, and also 0.67 mg/100 g of carotenoids. The application of UF has proved to be viable both in the recovery of valuable compounds and in the decontamination of water, contributing to the sustainability of the fish productive sector.
DOI: 10.6084/m9.figshare.14318194.v1
title: Ultrafiltration for the recovery of proteins from surimi washing water

description: Abstract The aim of this study was to evaluate the efficiency of the ultrafiltration process (UF) in the cleansing treatment of the tilapia surimi washing water and in the recovery of components. After producing surimi, the water generated in the washing operations was subjected to UF in a 30 kDa polyethersulfone membrane. Unfiltered washing waters and UF permeate and concentrate were analyzed for pH, total solids (TS) and fixed solids (FS), total proteins, and lipids. The chemical oxygen demand (COD) was determined in the original water and in the permeate. The concentrate was dehydrated in a spray dryer and analyzed for amino acids and carotenoids. The permeate showed a significant decrease in TS, proteins and COD, indicating that UF was efficient in removing the organic load from the waters. The percentage of protein recovery by UF was 93.12% and the dehydrated concentrate had all the essential amino acids, being leucine (5.47%), lysine (6.49%), valine (3.59%), and phenylalanine (3.68%) those with the highest concentrations, and also 0.67 mg/100 g of carotenoids. The application of UF has proved to be viable both in the recovery of valuable compounds and in the decontamination of water, contributing to the sustainability of the fish productive sector.
DOI: 10.6084/m9.figshare.14318198
title: Study on the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injury of mouse

description: Abstract To the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injuries under different levels of concentration. The process is as follows: (1) establish MCAO mouse model, test the modelling using traction and open field experiments, use TTC staining to detect the protective effect of Antelope horn treatment on cerebral ischemia reperfusion injury; (2) after 48 h of MCAO, use Nissl staining to measure the extent of hippocampal neuron damage in each group. The hippocampus corner 1 sector and dentate gyrus corner zone of hippocampal neurons of ischemia reperfusion group mouse suffered severe damages under Nissl staining detection. However, the hippocampal neurons of the medicated mouse show obvious alleviation. Western Blot results showed that, compared to the ischemic control group, the Bcl-2, Hsp 27, Hsp 27 expressions of the medicated group were significantly increased, while the Bax expression was dramatically reduced. Immunofluorescence outcome showed obvious reduction of TUNEL and FJB positive cells in the medicated group. That Antelope horn can reduce focal cerebral ischemia reperfusion injury. The reduction of degeneration and apoptosis suggests the correlation between the therapeutic effect of Antelope horn on ischemia reperfusion injury and its anti-apoptotic protective effect.
DOI: 10.6084/m9.figshare.14318198.v1
title: Study on the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injury of mouse

description: Abstract To the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injuries under different levels of concentration. The process is as follows: (1) establish MCAO mouse model, test the modelling using traction and open field experiments, use TTC staining to detect the protective effect of Antelope horn treatment on cerebral ischemia reperfusion injury; (2) after 48 h of MCAO, use Nissl staining to measure the extent of hippocampal neuron damage in each group. The hippocampus corner 1 sector and dentate gyrus corner zone of hippocampal neurons of ischemia reperfusion group mouse suffered severe damages under Nissl staining detection. However, the hippocampal neurons of the medicated mouse show obvious alleviation. Western Blot results showed that, compared to the ischemic control group, the Bcl-2, Hsp 27, Hsp 27 expressions of the medicated group were significantly increased, while the Bax expression was dramatically reduced. Immunofluorescence outcome showed obvious reduction of TUNEL and FJB positive cells in the medicated group. That Antelope horn can reduce focal cerebral ischemia reperfusion injury. The reduction of degeneration and apoptosis suggests the correlation between the therapeutic effect of Antelope horn on ischemia reperfusion injury and its anti-apoptotic protective effect.
DOI: 10.6084/m9.figshare.14318203.v1
title: Antimicrobial activity of Wickerhamomyces anomalus mycocins against strains of Staphylococcus aureus isolated from meats

description: Abstract Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contaminated with S. aureus coagulase positive is considered a public health risk because the bacterium is resistant to several conventional antimicrobials. Mycocins are substances produced by yeasts that secrete glycoproteins, which can also be called toxin killers, they have an inhibitory action on other microorganisms. The Wickerhamomyces anomalus, is one of the microorganisms capable of producing these mycocins, resulting in the action of disturbances on the cell wall of the pathogen causing deleterious effects. This work aims to evaluate the antimicrobial activity of the mycocins produced by W. anomalus WA45 against the 29 strains of S. aureus coagulase positive isolated from bovine, porcine and chicken meat and 1 standard strain. The antimicrobial action of the mycocins present on the culture supernatant of W. anomalus WA45 was tested by microdilution and the results were satisfactory, since 100% inhibition of strains of S. aureus coagulase positive. We concluded that the mycocins present in the supernatant of W. anomalus WA45 showed antimicrobial action, being candidates for the development of new products for the biocontrol and bioconservation of meat.