cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/bbcb8-15h52
title: H.pylori 26695 delta-cagH / AGS

description: Grid box YWC100
description: <strong>Tilt Series Date:</strong> 2014-04-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.7. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 2 sec. Ethane/propane mix.</p>
  ycw2014-04-14-19_slicer6888.jpg: Chemoreceptors ycw2014-04-14-19_slicer6889.jpg: Type 6 secretion system .  T6SS
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-14-19/rawdata/</p>20140414_YWC100_30008.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-14-19/rawdata/20140414_YWC100_30008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140414_YWC100_30008_part131_1.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-14-19/3dimage_41616/20140414_YWC100_30008_part131_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.cyptd5
title: Occurrence Download

description: A dataset containing 120 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Bromus lanceolatus",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 120 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0011960-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.3535/ejq-fcz-6qs
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/dx1-mj9-54e
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/g3k-411-8bv
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/gtbp2-mdz39
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-86/rawdata/</p>20130928_YWC034_10094.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-86/rawdata/20130928_YWC034_10094.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10094.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-86/3dimage_31526/20130928_YWC034_10094.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mnb6j-bqc45
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-69/rawdata/</p>20130928_YWC034_10069.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-69/rawdata/20130928_YWC034_10069.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10069.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-69/3dimage_31494/20130928_YWC034_10069.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/cp7-gat-h61
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hqd-t0l-emx
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/mm9-zc5-lg5
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/6xa-yps-mt3
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gx2-hrn-7yk
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yy0-tqf-8wg
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9m1-mhn-hk0
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/nnd-gwh-t6g
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/red-tqq-dl4
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jpe-3ap-kz4
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/yk5-epm-cxs
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/xsz00-8qr40
title: H. pylori 26695 / AGS

description: Grid box YWC054
description: <strong>Tilt Series Date:</strong> 2013-11-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
  ycw2013-11-25-43_slicer10087.jpg: Helicobacter pylori .  flat vesicle ycw2013-11-25-43_slicer17899.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-43/rawdata/</p>20131125_YWC054_10043.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-43/rawdata/20131125_YWC054_10043.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131125_YWC054_10043_part121_5.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-43/3dimage_35099/20131125_YWC054_10043_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/lhy-g03-qj9
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/mpx-cky-m35
title: Cnidaria

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/b1v-0q5-8hj
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jhq-fqs-t19
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ts3-pj5-5nd
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/8db-ss5-btr
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/h36-6pv-bhx
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/zf7-apq-bjv
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/3nq-kwy-a2h
title: Coleoidea Bather, 1888

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/62t-8jw-dse
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/bdp-zgw-9ne
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fma-jaf-30x
title: †Ammonoidea

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/7345c-q5m34
title: H. pylori 26695 / 20 nm gold-DNA

description: Grid box LR005
description: <strong>Tilt Series Date:</strong> 2014-01-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 3 days, OD600=0.4.  Spun cells down at 3000rpm for 5 minutes to concentrate before using; resuspended pellet.</p>
<strong>Sample Preparation:</strong> Combined 20ul cells with 10ul 10 nm gold fiducials (100ul gold and 25ul BSA) and 10ul 20 nm streptavidin gold-biotin DNA.  Grids frozen 7-20 minutes after combining DNA labeled gold with concentrated cells.  Vitrobot settings: 100% humidity, 22.5 C, -3 offset, 4.5 sec blot time, Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-01-21-15/rawdata/</p>20140121_LR005_20010.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-01-21-15/rawdata/20140121_LR005_20010.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140121_LR005_20010_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-01-21-15/3dimage_37906/20140121_LR005_20010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9c659-yjk75
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-03-31-25_slicer5942.jpg: Flat vesicles ycw2014-03-31-25_slicer5943.jpg: Flat vesicle ycw2014-03-31-25_slicer5945.jpg: Filaments
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-25/rawdata/</p>20140331_YWC097_10025.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-25/rawdata/20140331_YWC097_10025.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10025_full.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-25/3dimage_40668/20140331_YWC097_10025_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.3535/mt3-gd6-ptp
title: Ostracoda Latreille, 1802

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/p3d-wqq-e47
title: Echinodermata

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/wvl-90z-7bm
title: Gastropoda Cuvier, 1795

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/z31-dat-qft
title: Animalia

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.22002/85zxp-1vy09
title: E.coli co-overexpression

description: E.coli co overexpression UU1607/pJC3/pPM25
description: <strong>Tilt Series Date:</strong> 2013-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain sent from Heather Hodges (Kiessling lab), parent strain and plasmids published from the Parkinson lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew UU1607/pJC3/pPM25 overnight in 5mL TB + 3.8µl Cm + 5µl CarbDiluted back 1:50 into 200mL TB + 152µl Cm + 200µl Carb + 50µl 1M IPTG + 200µl Na-salicylateGrew ~2 hours to mid-log phaseSpun down 10' at 5,000 x gResuspended in 300µl lysis buffer + 0.1µl PMSF + 0.75 µl 1,10-phenanthrolineAdded 33.3µl (2mg/ml) fresh lysozyme solution (20mg/ml in 10mM Tris-HCl, pH 8.0)Incubated 45' at 37°CAdded 1.67µl 1M MgCl2, 0.2µl 1M CaCl2, 0.33µl DNase IIncubated 30' at 37°CSpun down 4000 g 5 min Resuspended in activity buffer (cold room) without glycerol</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-9/rawdata/</p>cooverexpressionatlas4micronb0009.mrc,  Tilt Series (Pixel Size 0.28 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-9/rawdata/cooverexpressionatlas4micronb0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        cooverexpressionatlas4micronb0009_part67_17.rec,  Reconstruction (Pixel Size 0.56 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-9/3dimage_28363/cooverexpressionatlas4micronb0009_part67_17.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/01brc-vf966
title: H.pylori 26695 delta-PAI / AGS

description: Grid box: YWC098
description: <strong>Tilt Series Date:</strong> 2014-04-01</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-27/rawdata/</p>20140401_YWC098_10027.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-27/rawdata/20140401_YWC098_10027.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140401_YWC098_10027_full.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-01-27/3dimage_40747/20140401_YWC098_10027_full.rec"
        > <i class="download icon"></i> Download </a></p>