DOI: 10.5061/dryad.8566p
title: Data from: Balancing genetic uniqueness and genetic variation in determining conservation and translocation strategies: a comprehensive case study of threatened dwarf galaxias, Galaxiella pusilla (Mack) (Pisces: Galaxiidae)

description: Genetic markers are widely used to define and manage populations of
 threatened species based on the notion that populations with unique
 lineages of mtDNA and well-differentiated nuclear marker frequencies
 should be treated separately. However a danger of this approach is that
 genetic uniqueness might be emphasized at the cost of genetic diversity,
 which is essential for adaptation and is potentially boosted by mixing
 geographically separate populations. Here we re-explore the issue of
 defining management units, focussing on a detailed study of Galaxiella
 pusilla, a small freshwater fish of national conservation significance in
 Australia. Using a combination of microsatellite and mitochondrial
 markers, 51 populations across the species range were surveyed for genetic
 structure and diversity. We found an inverse relationship between genetic
 differentiation and genetic diversity, highlighting a long-term risk of
 deliberate isolation of G. pusilla populations based on protection of
 unique lineages. Instead we adopt a method for identifying genetic
 management units that takes into consideration both uniqueness and genetic
 variation. This produced a management framework to guide future
 translocation and re-introduction efforts for G. pusilla which contrasted
 to the framework based on a more traditional approach that may overlook
 important genetic variation in populations.
description: Coleman et al dwarf
 galaxias microsatellite dataRaw microsatellite data
 using 10 loci and fin tissue samples collected from 51 dwarf galaxias
 populations throughout southeastern Australia.Coleman et al dwarf
 galaxias mtDNA haplotypesmtDNA haplotypes for
 16S, COI and CytB from fin tissue samples collected from 51 dwarf galaxias
 populations throughout southeast Australia. Haplotype sequences are
 available from Genbank.Coleman et al dwarf
 galaxias population detailsPopulation details for
 51 dwarf galaxias populations where fin tissue samples were collected
 throughout southeast Australia.Coleman et al. dwarf
 galaxias 16S haplotype sequences16S haplotype sequences
 from fin tissue samples collected from 51 dwarf galaxias populations
 throughout southeast Australia. These haplotype sequences are available
 from Genbank under accession numbers KC282524 (Hap1) - KC282538
 (Hap15).Coleman et al. Dwarf
 galaxias COI haplotype sequencesCOI haplotype sequences
 from fin tissue samples collected from 51 dwarf galaxias populations
 throughout southeast Australia. These haplotype sequences are available
 from Genbank under accession numbers KC282473 (Hap1)- KC282523
 (Hap51).Coleman et al. dwarf galaxias
 COI haplotype sequences.fasColeman et al. dwarf
 galaxias CytB haplotype sequencesCytB haplotype sequences
 from fin tissue samples collected from 51 dwarf galaxias populations
 throughout southeast Australia. These haplotype sequences are available
 from Genbank under accession numbers KC282539 (Hap1) - KC282620
 (Hap82).