DOI: 10.18150/guhr5e
title: Raw data obtained as the result of redox dye selection and inoculum conditions improvement experiments for the optimisation of respirometric indices in Verticillium and Trichodermandices in Verticillium and Trichoderma

description: The deposited data were collected as part of the research project entitled: “Różnorodność fenotypowa grzybów z rodzaju Verticillium i Trichoderma a konkurencja o azot - aspekt metodyczny” (Diversity of phenotypes of Verticillium and Trichoderma in relation to nitrogen competition - methodological aspect).This study was co-funded by the National Science Centre in the frame of the MINIATURA 5 scientific activity, contract number 2021/05/X/NZ9/00341 and the National Centre for Research and Development in the frame of the EcoFruits project, contract number BIOSTRATEG3/344433/16/NCBR/2018 (strains used in the experiments are part of the EcoFruits project). Understanding which carbon or nitrogen sources the competing fungal species prefer is pivotal for specific biotechnological applications. However, this is not straightforward, as each strain sometimes behaves differently under the experimental conditions adopted. To analyse the trophic overlap of two species, it is necessary to refine diagnostic techniques and exclude variables that may interfere with the measurements. A protocol for establishing the suitability of chromogenic dyes in the analysis of filamentous fungi with phenotype microarrays was described. The research goal was to determine the most suitable redox dye indicator and its optimal concentration that reacts quantitatively to the respiratory activity of both Verticillium spp. and Trichoderma spp. isolates in the presence of a nitrogen source. The commercial BiologTM Redox Dye Mixes D, E, and F and also TTC (2,3,5-Triphenyltetrazolium chloride), INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride), and MTT solutions were evaluated in selected concentrations. Both their nutritive and toxic effects were quantified. Results showed that 1% “F” and 0.5% “D” BiologTM dyes were appropriate for the functional evaluation of isolates belonging to the Verticillium and Trichoderma genera.The research was conducted from 14. February 2022, to 16. March 2022, at the Institute of Agrophysics, Polish Academy of Sciences, the Department of Soil and Plant System, Laboratory of Molecular and Environmental Microbiology.The data includes sets of biological data (characteristics of fungal strains - metabolic profile). These data were obtained through the measurement of the level of nitrogen substrate utilization by microorganisms, i.e. the respiratory response of the tested isolates resulting from substrate catabolism (colorimetric reaction) (490 nm) and the level of cell biomass growth (measured by optical density) (750 nm).The dataset consists of the following files:A1_medium – raw data regarding research results to select appropriate medium for fungal growth.A2_nutrition_D F E - raw data regarding research results to evaluate fungi's use of chromogenic compounds (D F E) as carbon and nitrogen sources.A3_nutrition_TTC- raw data regarding research results to evaluate fungi's use of chromogenic compounds (TTC) as carbon and nitrogen sources.A4_nutrition_INT MTT - raw data regarding research results to evaluate fungi's use of chromogenic compounds (INT MTT) as carbon and nitrogen sources.A5_toxicity_MTT INT - raw data regarding research results to evaluate the toxicity of chromogenic compounds (MTT INT) for fungi.A6_toxicity_D F - raw data regarding research results to evaluate the toxicity of chromogenic compounds (D F) for fungi.A7_toxicity_E - raw data regarding research results to evaluate the toxicity of chromogenic compound (E) for fungi.A8 - Version of record of publication: Selection of redox dye and inoculum conditions for the optimisation of respirometric indices in Verticillium and TrichodermaThe data were compiled based on conducted laboratory research. The results presented in the files were obtained using research equipment: a microplate reader with a computer workstation - MicroStationTM (BiologTM Hayward, USA).