DOI: 10.22002/0n3gs-ezs85
title: S. pombe Cryosection

description: cryosection, pombe, actomyosin, actin, myosin
description: <strong>Tilt Series Date:</strong> 2013-09-23</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1 degree°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized for 4 hrs at 36 Celsius, then temp shifted to 25 for ~50 min until continuous rings are seen in most cells. </p>
<strong>Sample Preparation:</strong> cryosection</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/</p>092313_pombe_01.mrc,  Tilt Series (Pixel Size 0.6167 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe_01.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0001.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0002.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0004.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0005.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0006.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0007.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0009.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        092313_pombe0010.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0010.mrc"
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        092313_pombe0011.mrc,  Tilt Series (Pixel Size 0.6167 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-23-1/rawdata/092313_pombe0011.mrc"
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