DOI: 10.5061/dryad.rn8pk0pmc
title: Higher levels of cardiovascular biomarkers following hypertensive compared to normotensive pregnancy

description: Hypertensive disorder of pregnancy (HDP) is associated with an increased
 risk for later-life cardiovascular disease (CVD). Whether the HDP
 pregnancy itself confers risk toward CVD later in life is suggested in
 several epidemiologic studies. Given this connection and that the HDP
 exposure itself may play a role, understanding whether markers associated
 with cardiovascular risk vary based on HDP history in the years following
 pregnancy may assist with risk stratification and development of targeted
 interventions. We measured 77 proteins (CVD-associated and inflammatory
 markers) in n = 22 individuals with a history of HDP and n = 42 matched
 controls with no HDP history at a median of 4 years after pregnancy.
 Several CVD-associated proteins (fibrinogen, fetuin-A, L-selectin, and
 alpha-1-acid glycoprotein) were significantly elevated, by orders of
 magnitude, in individuals with a history of HDP compared to normotensive
 pregnancies (all p &lt; 0.0001). In multivariable linear regression
 models controlling for age, body mass index, chronic hypertension, and
 diabetes, a history of HDP remained associated with higher levels of
 CVD-associated proteins (all p &lt; 0.0001). We clustered samples
 based on global patterns of CVD protein expression and found a significant
 difference in CVD protein expression patterns between post-Normal and
 post-HDP samples. Conversely, differences in circulating inflammatory
 markers were largely insignificant or more subtle than those observed with
 the CVD-associated proteins. Identification of biomarkers associated with
 CVD in the intervening years after HDP but before evident CVD is critical
 to understanding post-HDP cardiovascular risk to provide insight for the
 development of therapeutic interventions that mitigate CVD event risk in
 this high-risk population.
description: Clinical metadata was abstracted from electronic health records
 by trained obstetric providers. 
description: # Higher levels of cardiovascular biomarkers following hypertensive
 compared to normotensive pregnancy
 [https://doi.org/10.5061/dryad.rn8pk0pmc](https://doi.org/10.5061/dryad.rn8pk0pmc) Results are from a case-control study of participants several years after a pregnancy complicated by a hypertensive disorder of pregnancy (HDP) or prior normotensive pregnancies. The post-HDP cohort includes n=22 participants, while the post-Normal cohort includes n=42 participants.  ## Description of the data and file structure 1. *deidentified_metadata.xls*: Excel file containing de-identified clinical metadata for all participants.  2. *deidentified_metadata_codebook.xls*: Codebook for variables in "deidentified_metadata.xls". Note that cells with "." indicate missing data.  3. *protein_concentration_legend.xlsx*: Excel file containing legend for raw protein concentration values.  4. *cvd_proteins_1_plasma.xlsx*: Excel file containing raw concentration values of 6 cardiovascular disease (CVD)-associated proteins. 5. *cvd_protein_2_plasma.xlsx*: Excel file containing raw concentration values of 9 CVD-associated proteins.  6. *cytokine_proteins_plasma.xlsx*: Excel file containing raw concentration values of 48 cytokines.  7. *soluble_cytokine_receptor_proteins_plasma.xlsx*: Excel file containing raw concentration values of 14 soluble cytokine receptors.  All protein concentrations are measured in pg/mL except for the following proteins, which are measured in ng/mL: alpha-2 microglobulin, AGP, Fetuin-A36, Fibrinogen, Haptoglobin, and PF4. ## Human subjects data We received informed consent from participants to publish the de-identified data in the public domain. All data is de-identified. Each participants is noted with an anonymized studyID and no direct identifiers are included.