cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9697133
title: Effect of Inoculation of Pineapple Plantlets with Arbuscular Mycorrhizal Fungi Obtained from Different Inoculum Sources Multiplied by the On-Farm Method

description: ABSTRACT The production of inoculum from arbuscular mycorrhizal fungi (AMF) at a large scale and low cost is essential for establishing methods to assist in producing pineapple plantlets with high nutritional and phytosanitary quality. However, this objective is difficult to accomplish because of the biotrophic nature of these fungi. The on-farm multiplication method for AMF inoculum presents a good alternative to supply the demand for the production of glomerospores. This study aimed to multiply and evaluate AMF inoculum originating from isolated species (including Rhizophagus clarus, Claroideoglomus etunicatum) versus native AMF from pineapple and coffee plantations multiplied by the on-farm method on the colonization in pineapple plantlets. Initially, inocula of R. clarus, C. etunicatum, and native AMF (pineapple and coffee) were multiplied by the on-farm method in Sorghum bicolor. After four months, the number of AMF spores and the percentage of viable spores at the layers of 0.00-0.05 and 0.05-0.10 m were evaluated. There were no differences in spore numbers in relation to the source of the inoculum (R. clarus, C. etunicatum, pineapple, and coffee) and evaluated layers, with an average number of 605 spores per 100 cm3 of soil. The percentage of viable spores was greater at the layer of 0.00-0.05 m (76.32 %) compared to the layer of 0.05-0.10 m (72.05 %), regardless of the inoculum source. The viability of the inoculum obtained from C. etunicatum was higher than that from the coffee crop (77.93 and 68.06 %, respectively). Subsequently, the spores were inoculated in pineapple plantlets to assess the rate of colonization. Pineapple plantlets inoculated with AMF had an average of colonization of 18 and 67.73 % after 50 and 180 days cultivation, respectively, with no significant difference being detected between treatments. Therefore, on-farm inoculum production was effective at multiplying the AMF of both isolates of R. clarus and C. etunicatum, as well as for commercial crops (pineapple and coffee), with spores having high viability. Arbuscular mycorrhizal fungi colonized pineapple plantlets independently of the inoculum utilized and favored its growth.
DOI: 10.6084/m9.figshare.9697136
title: The effect of panretinal photocoagulation on confocal laser scanning ophthalmoscopy and stereo photographic parameters of optic disk topography in patients with diabetic retinopathy

description: ABSTRACT Purpose: To determine the effect of panretinal photocoagulation on optic disk topographic parameters in non-glaucomatous patients with proliferative diabetic retinopathy. Methods: This was a prospective, single-center, observational study. Thirty-eight eyes of 26 patients with diabetes underwent panretinal photocoagulation for proliferative diabetic retinopathy. Stereoscopic disk photographs and optic nerve head parameters were evaluated using the Zeiss fundus camera and the confocal scanning laser ophthalmoscope (Heidelberg Retinal Tomograph), respectively, at baseline and 12 months after the completion of panretinal photocoagulation. Results: Thirty-eight eyes of 26 patients (15 female) with a mean age of 53.7 (range 26-74) years were recruited. No significant difference was found between the stereo photography determined mean horizontal and vertical cup-to-disk ratio before and after panretinal photocoagulation treatment (p=0.461 and 0.839, respectively). The global values of the optic nerve head parameters analyzed with the HRT3 showed no significant change from baseline to 12 months, including the disk area, cup area, rim area, cup volume, rim volume, cup-to-disk area ratio, linear cup-to-disk ratio, mean cup depth, maximum cup depth, cup shape measure, height variation contour, mean retinal nerve fiber layer thickness, and cross-sectional area. Conclusion: Our results suggest that panretinal photocoagulation does not cause morphological optic disk changes in patients with diabetic proliferative retinopathy after 1 year of follow-up.
DOI: 10.6084/m9.figshare.9697139
title: Identification of aroma-active volatile compounds in Pouteria sapota fruit by aroma extraction dilution analyses (AEDA)

description: Pouteria sapota is a tropical fruit that is commonly found in central and north part of South America where is widely consumed by its biofunctional properties. The aroma active volatile compounds of Pouteria sapota fruit were studied by using the molecular sensory approach, which includes a SAFE (Solvent Assisted Flavor Evaporation) distillation to get a volatile extract that resembles the fruit aroma, and the combination of GC-O (Gas Chromatography coupled to Olfactometry) with GC-MS analyses to identify odour-active compounds. By AEDA (Aroma Extract Dilution Analysis), β-damascenone, furaneol, linalool, (Z)-3-hexenal, and benzaldehyde, were identified as key aroma compounds in this fruit.
DOI: 10.6084/m9.figshare.9697145.v1
title: Field Perception of the Boundary Between Soil and Saprolite by Pedologists and its Differentiation Using Mathematical Models

description: ABSTRACT Saprolite plays a central role into hydrologic and nutrient cycles. Despite that, saprolite research is scattered and uses heterogeneous, sometimes conflicting, methods and concepts. During field work, it is difficult to assign the boundary between soil and saprolite. This paper aimed to identify the subjacent logic that pedologists use to assign to a regolith volume its soil or saprolite nature. To achieve this goal, a tree algorithm was used to build a hierarchy of physical and chemical properties of a set of regolith profiles. Such hierarchization expose the inner, subjective criteria used by researchers during the assignment of a certain profile zone as saprolite or soil. The following variables were measured: total porosity (TP); bulk density (Bd); particle density (Pd); total Fe2O3, Al2O3, CaO, MgO, K2O, Na2O, P2O5, and TiO2; selective extraction of iron by ditionite-citrate-bicarbonate (FeDCB) and ammonium oxalate (FeOA); and the FeDCB/FeOA ratio. These measurements were done in a set of 25 regolith profiles (137 horizons and layers), located in the Southeast region and Northeast region of Brazil. The decision tree algorithm was applied using the recursive partition method to identify which of the measured property was most strongly associated with the field assignment of the pedologists to a certain profile zone as saprolite or soil. The Bd, FeDCB/FeOA, MgO, CaO, TP, and P2O5 explained 93 % of the pedologists choice, being Bd responsible for 81 %.
DOI: 10.6084/m9.figshare.9697130
title: Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus

description: ABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis. Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the de novo peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes. Conclusions: Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland.
DOI: 10.6084/m9.figshare.9697136.v1
title: The effect of panretinal photocoagulation on confocal laser scanning ophthalmoscopy and stereo photographic parameters of optic disk topography in patients with diabetic retinopathy

description: ABSTRACT Purpose: To determine the effect of panretinal photocoagulation on optic disk topographic parameters in non-glaucomatous patients with proliferative diabetic retinopathy. Methods: This was a prospective, single-center, observational study. Thirty-eight eyes of 26 patients with diabetes underwent panretinal photocoagulation for proliferative diabetic retinopathy. Stereoscopic disk photographs and optic nerve head parameters were evaluated using the Zeiss fundus camera and the confocal scanning laser ophthalmoscope (Heidelberg Retinal Tomograph), respectively, at baseline and 12 months after the completion of panretinal photocoagulation. Results: Thirty-eight eyes of 26 patients (15 female) with a mean age of 53.7 (range 26-74) years were recruited. No significant difference was found between the stereo photography determined mean horizontal and vertical cup-to-disk ratio before and after panretinal photocoagulation treatment (p=0.461 and 0.839, respectively). The global values of the optic nerve head parameters analyzed with the HRT3 showed no significant change from baseline to 12 months, including the disk area, cup area, rim area, cup volume, rim volume, cup-to-disk area ratio, linear cup-to-disk ratio, mean cup depth, maximum cup depth, cup shape measure, height variation contour, mean retinal nerve fiber layer thickness, and cross-sectional area. Conclusion: Our results suggest that panretinal photocoagulation does not cause morphological optic disk changes in patients with diabetic proliferative retinopathy after 1 year of follow-up.
DOI: 10.6084/m9.figshare.9697124
title: Hydrostatic Equilibrium between Soil Samples and Pressure Plates Used in Soil Water Retention Determination: Consequences of a Questionable Assumption

description: ABSTRACT Soil water retention is among the soil hydraulic properties most routinely measured in studies of soil physics and related areas. This property is used in dynamic simulations of vadose zone processes such as soil water availability, surface boundary processes of evaporation and infiltration, and the fate of soil pollutants. The most common measurement technique consists in establishing a hydrostatic equilibrium between an initially saturated soil sample and a porous medium at a certain tension on a tension table or pressure plate. However, there is reasonable doubt about the assumed hydrostatic equilibrium, especially in the dry range at low pressure heads. In this study we compared the traditional pressure plate apparatus protocol to an inverse parameter estimation protocol based on a transient evaporation experiment. Independent pressure head measurements using a dewpoint device were also performed. We sampled a variety of soil textures typical of the Brazilian subtropical humid zones, aiming to show differences between textures in their subjection to hydraulic nonequilibrium. The performed experiments allow to conclude that the two compared protocols showed real pressure heads in samples on a pressure plate to be less negative than the assumed ones, leading to an overestimation of the soil water content in the dry range at low pressure heads, especially in fine-textured soil samples. This affects the reliability of most soil hydraulic databases, derived PTFs in the dry range, as well as the wilting point estimation. Water availability predictions based on total and readily available water are more sensitive to the water retention measurement method when the chosen lower limits of available water are closer to the wilting point. In this sense, irrigation timing criteria based on readily available water should be preferred over total available water, especially for fine-textured soils. Finally, given the low reliability of the pressure plate apparatus for low pressure heads, possibly biasing hydrological simulations and their interpretation, alternative measurement methods for the drier part of the soil water retention curve should be preferred, e.g., the proposed inverse modeling of evaporation experiments.
DOI: 10.6084/m9.figshare.9697133.v1
title: Effect of Inoculation of Pineapple Plantlets with Arbuscular Mycorrhizal Fungi Obtained from Different Inoculum Sources Multiplied by the On-Farm Method

description: ABSTRACT The production of inoculum from arbuscular mycorrhizal fungi (AMF) at a large scale and low cost is essential for establishing methods to assist in producing pineapple plantlets with high nutritional and phytosanitary quality. However, this objective is difficult to accomplish because of the biotrophic nature of these fungi. The on-farm multiplication method for AMF inoculum presents a good alternative to supply the demand for the production of glomerospores. This study aimed to multiply and evaluate AMF inoculum originating from isolated species (including Rhizophagus clarus, Claroideoglomus etunicatum) versus native AMF from pineapple and coffee plantations multiplied by the on-farm method on the colonization in pineapple plantlets. Initially, inocula of R. clarus, C. etunicatum, and native AMF (pineapple and coffee) were multiplied by the on-farm method in Sorghum bicolor. After four months, the number of AMF spores and the percentage of viable spores at the layers of 0.00-0.05 and 0.05-0.10 m were evaluated. There were no differences in spore numbers in relation to the source of the inoculum (R. clarus, C. etunicatum, pineapple, and coffee) and evaluated layers, with an average number of 605 spores per 100 cm3 of soil. The percentage of viable spores was greater at the layer of 0.00-0.05 m (76.32 %) compared to the layer of 0.05-0.10 m (72.05 %), regardless of the inoculum source. The viability of the inoculum obtained from C. etunicatum was higher than that from the coffee crop (77.93 and 68.06 %, respectively). Subsequently, the spores were inoculated in pineapple plantlets to assess the rate of colonization. Pineapple plantlets inoculated with AMF had an average of colonization of 18 and 67.73 % after 50 and 180 days cultivation, respectively, with no significant difference being detected between treatments. Therefore, on-farm inoculum production was effective at multiplying the AMF of both isolates of R. clarus and C. etunicatum, as well as for commercial crops (pineapple and coffee), with spores having high viability. Arbuscular mycorrhizal fungi colonized pineapple plantlets independently of the inoculum utilized and favored its growth.
DOI: 10.6084/m9.figshare.9697127
title: Effect of iodonium salt and chitosan on the physical and antibacterial properties of experimental infiltrants

description: Abstract Resinous infiltrants are indicated in the treatment of incipient carious lesions, and further development of these materials may contribute to greater control of these lesions. The aim of this study was to analyze the physical and antibacterial properties of experimental infiltrants containing iodonium salt and chitosan. Nine experimental infiltrants were formulated by varying the concentration of the diphenyliodonium salt (DPI) at 0, 0.5 and 1 mol%; and chitosan at 0, 0.12 and 0.25 g%. The infiltrants contained the monomeric base of triethylene glycol dimethacrylate and bisphenol-A dimethacrylate ethoxylate in a 75 and 25% proportion by weight, respectively; 0.5 mol% camphorquinone and 1 mol% ethyl 4-dimethylaminobenzoate. The degree of conversion was evaluated using Fourier transformer infrared spectroscopy, and the flexural strength and elastic modulus using the three-point bending test. Sorption and solubility in water, and antibacterial analysis (minimum inhibitory concentration and minimum bactericidal concentration) were also analyzed. Data was analyzed statistically by two-way ANOVA and Tukey’s test (p&lt;0.05), with the exception of the antibacterial test, which was evaluated by visual inspection. In general, the infiltrant group containing 0.5% DPI and 0.12% chitosan showed high values of degree of conversion, higher values of elastic modulus and flexural strength, and lower sorption values in relation to the other groups. Antibacterial activity was observed in all the groups with DPI, regardless of the concentration of chitosan. The addition of DPI and chitosan to experimental infiltrants represents a valid option for producing infiltrants with desirable physical and antibacterial characteristics.
DOI: 10.6084/m9.figshare.9697124.v1
title: Hydrostatic Equilibrium between Soil Samples and Pressure Plates Used in Soil Water Retention Determination: Consequences of a Questionable Assumption

description: ABSTRACT Soil water retention is among the soil hydraulic properties most routinely measured in studies of soil physics and related areas. This property is used in dynamic simulations of vadose zone processes such as soil water availability, surface boundary processes of evaporation and infiltration, and the fate of soil pollutants. The most common measurement technique consists in establishing a hydrostatic equilibrium between an initially saturated soil sample and a porous medium at a certain tension on a tension table or pressure plate. However, there is reasonable doubt about the assumed hydrostatic equilibrium, especially in the dry range at low pressure heads. In this study we compared the traditional pressure plate apparatus protocol to an inverse parameter estimation protocol based on a transient evaporation experiment. Independent pressure head measurements using a dewpoint device were also performed. We sampled a variety of soil textures typical of the Brazilian subtropical humid zones, aiming to show differences between textures in their subjection to hydraulic nonequilibrium. The performed experiments allow to conclude that the two compared protocols showed real pressure heads in samples on a pressure plate to be less negative than the assumed ones, leading to an overestimation of the soil water content in the dry range at low pressure heads, especially in fine-textured soil samples. This affects the reliability of most soil hydraulic databases, derived PTFs in the dry range, as well as the wilting point estimation. Water availability predictions based on total and readily available water are more sensitive to the water retention measurement method when the chosen lower limits of available water are closer to the wilting point. In this sense, irrigation timing criteria based on readily available water should be preferred over total available water, especially for fine-textured soils. Finally, given the low reliability of the pressure plate apparatus for low pressure heads, possibly biasing hydrological simulations and their interpretation, alternative measurement methods for the drier part of the soil water retention curve should be preferred, e.g., the proposed inverse modeling of evaporation experiments.
DOI: 10.6084/m9.figshare.9697130.v1
title: Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus

description: ABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis. Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the de novo peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes. Conclusions: Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland.
DOI: 10.6084/m9.figshare.9697139.v1
title: Identification of aroma-active volatile compounds in Pouteria sapota fruit by aroma extraction dilution analyses (AEDA)

description: Pouteria sapota is a tropical fruit that is commonly found in central and north part of South America where is widely consumed by its biofunctional properties. The aroma active volatile compounds of Pouteria sapota fruit were studied by using the molecular sensory approach, which includes a SAFE (Solvent Assisted Flavor Evaporation) distillation to get a volatile extract that resembles the fruit aroma, and the combination of GC-O (Gas Chromatography coupled to Olfactometry) with GC-MS analyses to identify odour-active compounds. By AEDA (Aroma Extract Dilution Analysis), β-damascenone, furaneol, linalool, (Z)-3-hexenal, and benzaldehyde, were identified as key aroma compounds in this fruit.
DOI: 10.6084/m9.figshare.9697121
title: Wide-field angiography for diagnosis and follow-up of Eales disease: a case series

description: ABSTRACT Wide-field angiography enables assessing peripheral areas with better quality and gives greater deep focus, which improves the image periphery. Some studies have proposed the usefulness of these angiographic systems in inflammatory diseases of the retina. However, few studies have evaluated this technique in Eales disease. We present a case series in which 5 eyes of 3 patients with Eales disease were evaluated by using retinal fluorescein angiography with 30º, 50º, and 150º lenses in a laser-scanning ophthalmoscope. These cases highlight the usefulness of wide-field fluorescein angiography in the diagnosis and follow-up of peripheral ischemic retinal areas in Eales disease, which enables better follow-up than possible with conventional fluorescein angiography images.