DOI: 10.6084/m9.figshare.24395053
title: Dataset: Raw fit data of both epithelial tissues and electronic cell models

description: This dataset was the result of running the 3P-EIS data through the software developed for this project (https://github.com/colbyNIH/3P-EIS). Each row corresponds to a unique measurement that was run through the fitting code. The column naming conventions are:RsolA, RsolB, Ra, Ca, Rb, Cb, Rs: These names correspond to the independently verified electronic circuit element, if it was known for a particular measurementVa_rail: a built in check for voltages near the maximum range of the hardware. This was used to check for incorrect hardware configurations.chamber: "model" or "Ussing." Model corresponds to the electronic model cell and Ussing corresponds to recordings from epithelial tissues mounted in the Ussing chamber.circuitValuesKnown: A boolean used for filtering only measurements where the ground truth elements were recorded.constrain_min_and_max_f: a boolean used to indicate if a minimum and maximum frequency range was enforced for each measurement in the fitting code. The magnitude of the min and max frequency were set in the software.cross_section: the cross-sectional area of the chamber in cm<sup>2</sup>.data_location: a string used to locate the raw data for each measured file.detrendSignal: a boolean to indicate if the detrending logic was applied to the measured voltages prior to performing the STFT analysis of the signal amplitudes.filterSingals: a boolean to indicate if the measured voltage data was low-pass filtered prior to STFT analysis of the signal amplitudes.fs: the sampling frequency of the voltage data in Hz.high_RSME: a boolean to check if the voltage data was extremely noisy, indicating a poor electrode connection during recording.variables with prefid "idx_best_" the index of the best fit out of all initial guesses for a particular model.import_error: a boolean to indicate if the measured voltage data failed to import correctly.include_in_analysis: a boolean used before all raw data had been acquired to test the fitting script logic. Mainly used to avoid loading files that didn't yet exist.manually_validated: a boolean used to show which files were checked by a human to match the expected ranges of voltages.meas_ID: a unique string generated at the start of any recording. Contains date time information about recording.meas_idx: for each recording, multiple measurements could be made. This value corresponds to the nth measurement in meas_ID.n_freqs: number of frequencies applied across the system for the particular EIS measurement. normlization_method: the string used in the fitting algorithm that set the pre-processing steps on the EIS data prior to fitting.prefix "p_": Ra, Rb, Rs, etc were converted to generic suffix numbers. The prefix "p" stands for parameter and matches one of the circuit elements. In this order: RsolA, RsolB, Ra, Ca, Rb, Cb, Rs links to 1, 2, 3, 4, 5, 6, and 7. prefix "pg_": the g in this variable prefix stands for "guess." This is a guess for the parameter with the same suffix number in "p." For example, pg_absEr_1 is the guess - based on the absEr model fit - for parameter p_1 and so on. The model used in this paper (membrane ratio) is "<b>absZr</b>."pgmax: the maximum value for each parameter suffix used in fitting.pgmin: the minimum value for each parameter suffix used in fitting.resnormbest: the sum of all residuals for the best fit with each model after fitting.signal_buffer_end: There was a delay in the NOVA 2.1.5 software at the beginning and end of the measurement. This is a user estimated value that corrects the true start and end of each recording so that the STFT windows search the correct portion of the time domain signal for the frequency of interest. signal_buffer_start: There was a delay in the NOVA 2.1.5 software at the beginning and end of the measurement. This is a user estimated value that corrects the true start and end of each recording so that the STFT windows search the correct portion of the time domain signal for the frequency of interest. total_guesses: the total number of random starting guesses used to fit each model to the measured data. vpipette_gain_adjust: for some of the validation data, the pipette gain was set to 10x and some of the data was set to 1x. To ensure the correct amplitude was calculated after STFT analysis, the raw voltage data was divided by this value.