cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21800639
title: Fig6G_PCR_MDCK_R1_LG389_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800639.v1
title: Fig6G_PCR_MDCK_R1_LG389_annotated.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800633
title: Fig6I_PCR_A549_R2_LG402_annotated.pdf

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800642
title: Fig6G-6I_PCR_MDCK_A549_R3_LG403-404.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800570
title: Fig5E_RACE_upstream-primer_R2_LG316_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800573
title: Fig5E_RACE_upstream-primer_R1_LG314.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800573.v1
title: Fig5E_RACE_upstream-primer_R1_LG314.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800633.v1
title: Fig6I_PCR_A549_R2_LG402_annotated.pdf

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800642.v1
title: Fig6G-6I_PCR_MDCK_A549_R3_LG403-404.sgd

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800645
title: Fig6G-6I_PCR_MDCK_A549_R3_LG403-404.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800645.v1
title: Fig6G-6I_PCR_MDCK_A549_R3_LG403-404.tif

description: RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted from viral stocks (G), supernatants from MDCK cells 48 hours post infection (H), or Xrn1 knock-out A549 cells 16 hours post-infection. Viruses analyzes included viruses harboring no insert, inserted WT STOML2 or mutant STOML2 sequences that is not cut by PA-X, either in the WT PR8 or PR8-PA(∆X) background. The RNA was then reverse-transcribed to cDNA and PCR amplified using primers located on either side of the STOML2 sequence to visualize on an agarose gel whether the STOML2 sequence was retained.
DOI: 10.6084/m9.figshare.21800570.v1
title: Fig5E_RACE_upstream-primer_R2_LG316_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800576
title: Fig5E_RACE_upstream-primer_R2_LG316.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800576.v1
title: Fig5E_RACE_upstream-primer_R2_LG316.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800582
title: Fig5E_RACE_upstream-primer_R1_LG314.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800582.v1
title: Fig5E_RACE_upstream-primer_R1_LG314.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800579
title: Fig5E_RACE_upstream-primer_R3_LG318_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800579.v1
title: Fig5E_RACE_upstream-primer_R3_LG318_annotated.tiff

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800585
title: Fig5E_RACE_upstream-primer_R1_LG314_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800585.v1
title: Fig5E_RACE_upstream-primer_R1_LG314_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800588
title: Fig5E_RACE_upstream-primer_R3_LG318.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800588.v1
title: Fig5E_RACE_upstream-primer_R3_LG318.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800450
title: Fig5E_RACE_downstream-primer_R1_LG314_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800450.v1
title: Fig5E_RACE_downstream-primer_R1_LG314_annotated.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800591
title: Fig5E_RACE_upstream-primer_R3_LG318.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800591.v1
title: Fig5E_RACE_upstream-primer_R3_LG318.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800594
title: Fig5E_RACE_upstream-primer_R2_LG316.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800594.v1
title: Fig5E_RACE_upstream-primer_R2_LG316.tif

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800453
title: Fig5E_RACE_downstream-primer_R1_LG314.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800453.v1
title: Fig5E_RACE_downstream-primer_R1_LG314.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.
DOI: 10.6084/m9.figshare.21800456
title: Fig5E_RACE_downstream-primer_R2_LG316.sgd

description: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would pick up both the 185 bp fragment due to a cut site within luciferase and if present, a 350 bp fragment originating from the inserted sequence.