cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/8zt8j-2dh49
title: Vibrio Yi-Wei style

description: Strain MKW1383 grown in conditions Yi-Wei uses for Bdellovibrio predation
description: <strong>Tilt Series Date:</strong> 2014-02-21</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown 24 hours in LB at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew at 30C for another 16 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-02-21-1/rawdata/</p>VibrioYiWeiStyle0001.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-02-21-1/rawdata/VibrioYiWeiStyle0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioYiWeiStyle0001_full.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-02-21-1/3dimage_39294/VibrioYiWeiStyle0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h6abj-qyv32
title: HUVECs infx pNLBg1I

description: Raw data files of HUVECs infx pNLBg1I
description: <strong>Tilt Series Date:</strong> 2013-10-19</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBglI. Sample frozen endpoint of virus infected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-30/rawdata/</p>13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0015.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-10-19-30/rawdata/13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        13oct19_C180-1_pNLBglI_Endpointvirus_AT2BT1_0015_part121_32.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.js7stx
title: Occurrence Download

description: A dataset containing 76655 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Myosotis discolor"
 ]
}

 The dataset includes 76655 records from 945 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047597-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.6xx83q
title: Occurrence Download

description: A dataset containing 108 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Hyacinthus orientalis",
 "Country is Austria",
 "HasCoordinate is true"
 ]
}

 The dataset includes 108 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047716-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/pgys3-r1n32
title: Agrobacterium tumefaciens C58

description: Agro C58
description: <strong>Tilt Series Date:</strong> 2013-10-23</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Induction for T-pilus with 200uM AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-10/rawdata/</p>c58_berk_home_195k0010.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-10/rawdata/c58_berk_home_195k0010.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_berk_home_195k0010_full.rec,  Reconstruction (Pixel Size 0.0 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-10/3dimage_49005/c58_berk_home_195k0010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wyzyd-a0c42
title: UU 2803- A413T delta P1P2

description: E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
description: <strong>Tilt Series Date:</strong> 2012-09-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown in TB to log phase, then lysed with Penicillin for one hour</p>
<strong>Sample Preparation:</strong> cells plunge frozen on R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-2/rawdata/</p>Coli2803atlas0917_10004.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-2/rawdata/Coli2803atlas0917_10004.mrc"
        > <i class="download icon"></i> Download </a></p>

        Coli2803atlas0917_10004_part130_4.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-2/3dimage_20810/Coli2803atlas0917_10004_part130_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.w7p2z7
title: Occurrence Download

description: A dataset containing 16165 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is one of (EOD – eBird Observation Dataset, iNaturalist Research-grade Observations)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "Geometry POLYGON((-80.4399 43.45367,-80.37948 43.45367,-80.37948 43.4859,-80.4399 43.4859,-80.4399 43.45367))"
 ]
}

 The dataset includes 16165 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047714-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.uqdebk
title: Occurrence Download

description: A dataset listing the 1264 species recorded in GBIF matching the query:
{
 "and" : [
 "CoordinateUncertaintyInMeters 0.0-100.0",
 "DatasetKey is iNaturalist Research-grade Observations",
 "GadmGid is USA",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Lecanoromycetes",
 "Year 2017-2024"
 ]
}

 The dataset's 1264 records were derived from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047702-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/mtxzx-5k806
title: JT5 on xylose

description: JT5
description: <strong>Tilt Series Date:</strong> 2014-07-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacteroidetes</p>
<strong>Strain:</strong> JT5-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Eric Matson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown on xylose as a carbon source</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
  etl2014-07-20-8_slicer20250.jpg: Unknown - Nicholas
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-07-20-8/rawdata/</p>JT5_xyl0008.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-07-20-8/rawdata/JT5_xyl0008.mrc"
        > <i class="download icon"></i> Download </a></p>

        JT5_xyl0008_full.rec,  Reconstruction (Pixel Size 0.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-07-20-8/3dimage_49408/JT5_xyl0008_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y5gvd-6ys82
title: Methanotroph

description: Methanotroph_starved
description: <strong>Tilt Series Date:</strong> 2013-06-22</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Methyloprofundus sedimenti</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Victoria Orphan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> starved</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-22-35/rawdata/</p>methano_starved0004.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-22-35/rawdata/methano_starved0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        methano_starved0004_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-06-22-35/3dimage_32268/methano_starved0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ac7df-3z567
title: HIV capsid purified

description: 80 A/2 41000 3 defocus Box B search track 13500 ,slit in 20eV
description: <strong>Tilt Series Date:</strong> 2013-12-05</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified HIV cores</p>
<strong>Sample Preparation:</strong> B box</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-23/rawdata/</p>41000df3hiv-capsidatlas1_30023.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-23/rawdata/41000df3hiv-capsidatlas1_30023.mrc"
        > <i class="download icon"></i> Download </a></p>

        41000df3hiv-capsidatlas1_30023_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-23/3dimage_35538/41000df3hiv-capsidatlas1_30023_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2vzbr-8yn65
title: HIV capsid purified trim5 alpha

description: 41000 6df, with slit 91/A2
description: <strong>Tilt Series Date:</strong> 2013-12-13</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 91.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified recombinant trim5 alpha decorated with decorated HIV cores</p>
<strong>Sample Preparation:</strong> B grid</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-29/rawdata/</p>Trim5alpha41000df60009.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-29/rawdata/Trim5alpha41000df60009.mrc"
        > <i class="download icon"></i> Download </a></p>

        Trim5alpha41000df60009.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 711.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-29/3dimage_47840/Trim5alpha41000df60009.sirt.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zm874-re352
title: HIV capsid

description: Box C, 0.5 exposure, 152, 41000 mag 3 defocus, slit 20 eV, search track 13500
description: <strong>Tilt Series Date:</strong> 2013-12-05</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 152.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> 3dmod, need to reconstruct with SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified HIV cores</p>
<strong>Sample Preparation:</strong> Box C</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-57/rawdata/</p>test_hivcapsid00024.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-57/rawdata/test_hivcapsid00024.mrc"
        > <i class="download icon"></i> Download </a></p>

        test_hivcapsid00024_part121_3.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-05-57/3dimage_35599/test_hivcapsid00024_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/05gep-d7k31
title: Bsubtilis_ponA_sacculi

description: Bsubtilis_sacculi_ponA
description: <strong>Tilt Series Date:</strong> 2012-02-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-27/rawdata/</p>MJsacculi0035.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-27/rawdata/MJsacculi0035.mrc"
        > <i class="download icon"></i> Download </a></p>

        MJsacculi0035_part82_9.rec,  Reconstruction (Pixel Size 1.9 nm), 877.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-27/3dimage_26185/MJsacculi0035_part82_9.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/71y0q-05x18
title: Agrobacterium delta rem

description: Agrobacterium tumefaciens non-motile delta rem mutant
description: <strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-46/rawdata/</p>delta_rem0033.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-46/rawdata/delta_rem0033.mrc"
        > <i class="download icon"></i> Download </a></p>

        delta_rem0033_full.rec,  Reconstruction (Pixel Size 2.24 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-46/3dimage_41048/delta_rem0033_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1719y-rmb08
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-12/3dimage_69383/</p>12may29_C135_1_MEFnomitoDNA_AT2BT20003_part82_19.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
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        12may29_C135_1_MEFnomitoDNA_AT2BT20003.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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