cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25574/23999
title: Chandra X-ray Observatory ObsId 23999

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/0ywes-qgw32
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter lysed n the presence of the crowding agent 10% Polyvinylpyrrolidone (PVP)
description: <strong>Tilt Series Date:</strong> 2013-10-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Protocol:-     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xg-     resuspended PVP in 80µL appropriate Buffer 2Buffer 1:Crowding agent1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0Buffer 2:Crowding agentQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-20/rawdata/</p>Rhodopvp27k0022.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-20/rawdata/Rhodopvp27k0022.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp27k0022SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 7.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-20/3dimage_46408/Rhodopvp27k0022SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24000
title: Chandra X-ray Observatory ObsId 24000

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/zrc08-7zy93
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-25-44_slicer10117.jpg: Helicobacter pylori .  chemoreceptor .  ribosome exclusion zone
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-44/rawdata/</p>20131025_Janelia_YWC043_10001.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-44/rawdata/20131025_Janelia_YWC043_10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_10001_part131_12.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-44/3dimage_33480/20131025_Janelia_YWC043_10001_part131_12.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24001
title: Chandra X-ray Observatory ObsId 24001

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/zszx9-sjy09
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2013-08-20</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett, Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
  ycw2013-08-20-18_slicer10315.jpg: nucleoid, Bdellovibrio bacteriovorus ycw2013-08-20-18_slicer14179.jpg: flagellar motor; flagellum; Bdellovibrio bacteriovorus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-18/rawdata/</p>20130820_Bdellovibrio+WM3433_10008.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-18/rawdata/20130820_Bdellovibrio+WM3433_10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130820_Bdellovibrio+WM3433_10008_part131_5.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-18/3dimage_29878/20130820_Bdellovibrio+WM3433_10008_part131_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24002
title: Chandra X-ray Observatory ObsId 24002

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.15468/dl.zpy6cx
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Cyphotilapia frontosa, Cyprichromis leptosoma, Cyprichromis microlepidotus, Cyrtocara moorii, Dagetichthys commersonnii, Synaptura commersonnii, Dagetichthys lakdoensis, Dalophis boulengeri, Dalophis cephalopeltis, Danakilia franchettii, Datnia elongata, Mesopristes elongatus, Denticeps clupeoides, Dermatopsoides kasougae, Dicentrarchus punctatus, Dicologlossa cuneata, Dimidiochromis compressiceps, Dimidiochromis dimidiatus, Dimidiochromis kiwinge, Dimidiochromis strigatus, Dinotopterus cunningtoni, Diplotaxodon aeneus, Diplotaxodon altus, Diplotaxodon argenteus, Diplotaxodon ecclesi, Diplotaxodon greenwoodi, Diplotaxodon limnothrissa, Diplotaxodon longimaxilla, Diplotaxodon macrops, Distichodus affinis, Distichodus altus, Distichodus antonii, Distichodus atroventralis, Distichodus brevipinnis, Distichodus decemmaculatus, Distichodus engycephalus, Distichodus fasciolatus, Distichodus hypostomatus, Distichodus kolleri, Distichodus langi, Distichodus lusosso, Distichodus maculatus, Distichodus mbiniensis, Distichodus microps, Distichodus mossambicus, Distichodus nefasch, Distichodus noboli, Distichodus notospilus, Distichodus petersii, Distichodus rostratus, Distichodus rufigiensis, Distichodus schenga, Distichodus sexfasciatus, Divandu albimarginatus, Docimodus evelynae, Docimodus johnstoni, Dolichallabes microphthalmus, Dolichamphilius brieni, Dolichamphilius longiceps, Dormitator lebretonis, Doumea angolensis, Doumea chappuisi, Doumea gracila, Doumea sanaga, Doumea thysi, Doumea typica, Drombus bontii, Gobius bontii, Dundocharax bidentatus, Ebomegobius goodi, Eclectochromis ornatus, Ectodus descampsii, Eleotris acanthopoma, Eleotris daganensis, Eleotris fusca, Eleotris mauritiana, Eleotris pellegrini, Eleotris senegalensis, Eleotris vittata, Eleotris vomerodentata, Ellochelon vaigiensis, Elops lacerta, Elops senegalensis, Engraulicypris bredoi, Engraulicypris brevianalis, Engraulicypris gariepinus, Engraulicypris moeruensis, Engraulicypris ngalala, Engraulicypris sardella, Engraulicypris spinifer, Enneacampus ansorgii, Enneacampus kaupi, Enteromius ablabes, Enteromius aboinensis, Enteromius afrohamiltoni, Enteromius afrovernayi, Enteromius aliciae, Enteromius aloyi, Enteromius amanpoae, Enteromius amatolicus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((19.5797 31.1722,19.1382 30.7949,19.1382 29.0599,28.1382 29.0599,28.1382 31.6271,27.0568 31.9369,25.941 32.1276,25.5225 32.0458,25.172 32.4321,23.6289 32.6801,23.3344 33.0794,22.2903 33.4173,21.7076 33.4407,20.3542 32.9952,19.684 32.4793,19.4254 31.8299,19.5797 31.1722))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008257-250827131500795/datasets/export for details.
DOI: 10.22002/t6z86-ya728
title: S. pombe (Cdc25-22)

description: Cryosections (180 nm nominal thickness)
description: <strong>Tilt Series Date:</strong> 2014-04-20</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized cytokinesis. From OD 600 = 0.25 grow for 4 hrs at 36 C. Return to 25 C for 45 min then begin monitoring ring formation by LM. Once 80-90 of rings are continuous they were mixed with Dextran (100 ul of 40% Dextran in ~ 400 ul of pelleted cells). They were then high pressure frozen.</p>
<strong>Sample Preparation:</strong> High pressure freezing followed by cryosectioning</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-24/3dimage_42083/</p>042014_pombe_0024.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-24/3dimage_42083/042014_pombe_0024.rec"
        > <i class="download icon"></i> Download </a></p>

        042014_pombe_0024.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-24/rawdata/042014_pombe_0024.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24003
title: Chandra X-ray Observatory ObsId 24003

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.25574/24009
title: Chandra X-ray Observatory ObsId 24009

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/78t9k-yqv04
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC106
description: <strong>Tilt Series Date:</strong> 2014-07-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.</p>
  ycw2014-07-07-28_slicer7437.jpg: nested vesicles in Myxococcus xanthus delta-tsaP .  extracellular vesicles ycw2014-07-07-28_slicer10399.jpg: nested vesicles .  Myxococcus xanthus .  snapshot by Megan
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-28/rawdata/</p>20140707_YWC106_10030.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-28/rawdata/20140707_YWC106_10030.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140707_YWC106_10030_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-28/3dimage_44430/20140707_YWC106_10030_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24010
title: Chandra X-ray Observatory ObsId 24010

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.25574/24011
title: Chandra X-ray Observatory ObsId 24011

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/jaqj4-a9j07
title: Myxococcus xanthus pilM-YFP

description: strain SA4088grid box YWC101
description: <strong>Tilt Series Date:</strong> 2014-05-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-05-16-23_slicer6220.jpg: secretion system? .  unknown tube? ycw2014-05-16-23_slicer6221.jpg: unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-23/3dimage_42770/</p>20140516_Janelia_YWC096_10045_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-23/3dimage_42770/20140516_Janelia_YWC096_10045_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20140516_Janelia_YWC096_10045.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-16-23/rawdata/20140516_Janelia_YWC096_10045.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24012
title: Chandra X-ray Observatory ObsId 24012

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/3xbtx-46661
title: S. pombe (Cdc25-22)

description: Cryosections (180 nm nominal thickness)
description: <strong>Tilt Series Date:</strong> 2014-04-19</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized cytokinesis. From OD 600 = 0.25 grow for 4 hrs at 36 C. Return to 25 C for 45 min then begin monitoring ring formation by LM. Once 80-90 of rings are continuous they were mixed with Dextran (100 ul of 40% Dextran in ~ 400 ul of pelleted cells). They were then high pressure frozen.</p>
<strong>Sample Preparation:</strong> High pressure freezing followed by cryosectioning</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-21/3dimage_42073/</p>041914_pombe_0021.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-21/3dimage_42073/041914_pombe_0021.rec"
        > <i class="download icon"></i> Download </a></p>

        041914_pombe_0021.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-21/rawdata/041914_pombe_0021.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.p6tjfe
title: Occurrence Download

description: A dataset containing 96323 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Ribes spicatum"
 ]
}

 The dataset includes 96323 records from 209 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008110-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25574/24013
title: Chandra X-ray Observatory ObsId 24013

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?
DOI: 10.22002/hpn3g-k7s54
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 2-4 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-03-13</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF TOMO</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 2-4 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-03-13-21/rawdata/</p>stiv4hatlas10017.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-03-13-21/rawdata/stiv4hatlas10017.mrc"
        > <i class="download icon"></i> Download </a></p>

        stiv4hatlas10017_full.rec,  Reconstruction (Pixel Size 2.52 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-03-13-21/3dimage_40148/stiv4hatlas10017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24016
title: Chandra X-ray Observatory ObsId 24016

description: Flows of hot Baryons in the Milky Way center: How are fountains & chimneys linked to the Galactic-scale outflow?