cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12853898
title: Development and characterisation of a monoclonal antibody to detect the mycotoxin roquefortine C

description: Roquefortine, also known as roquefortine C (ROQC) is a fungal secondary metabolite (mycotoxin) that is produced by some of the same Penicillia as the tremorgen penitrem-A (PEN-A). The two mycotoxins have been linked to sporadic cases of toxicosis in dogs, cattle, and humans, leading some to consider ROQC as a biomarker of PEN-A. Reported here are the development of a monoclonal antibody (mAb) and associated competitive enzyme-linked immunosorbent assay (ELISA) for the screening of ROQC in extracts of nuts (nut “milks”), and dog serum. The ELISA was sensitive for ROQC, with a level of 0.117 ng ml<sup>−1</sup> inhibiting colour development by 50% (IC<sub>50</sub>), a limit of detection of 0.026 ng ml<sup>−1</sup>, and a dynamic range (IC<sub>20</sub> to IC<sub>80</sub>) of 0.038 to 0.289 ng ml<sup>−1</sup> in buffer. The assay was tolerant to significant levels of methanol. Recoveries from 4 types of nut milks spiked over the range of 0.25 to 2 ng ml<sup>−1</sup> were in the range of 83.5% to 116%. A small survey of commercial nut “milks” and “creamers” indicated 4 of 35 samples contained ROQC at levels so low that they are unlikely to be significant to human health (&lt;0.6 ng ml<sup>−1</sup>). The assay was also applied to canine serum. Recoveries from serum spiked over the range of 0.2 to 5 ng ml<sup>−1</sup> ranged from 98.1% to 123%. The results suggest the ELISA can be applied to the screening of food products, such as nut extracts, as well as for the screening of serum from dogs suspected to be suffering from mycotoxin-induced tremors.
DOI: 10.6084/m9.figshare.12902698.v1
title: Determination of deoxynivalenol and its major conjugates in cereals using an organic solvent-free extraction and IAC clean-up coupled in-line with HPLC-PCD-FLD

description: A method for the determination of deoxynivalenol (DON) and its major conjugates in cereals was developed including an immunoaffinity column (IAC) clean-up coupled in-line with high-performance liquid chromatography, post-column derivatisation and fluorescence detection. An IAC for DON with cross-reactivity to 15-AcDON, 3-AcDON and DON-3-G enabled this approach. The isolated analytes were introduced into the chromatographic system without aliquotation employing the hot water elution technique, resulting in the desired low LOQ values for monitoring these analytes in cereals. The absence of any organic solvent during sample preparation in combination with an in-line IAC clean-up renders the method simple, fast, and environmentally friendly. Special attention was paid to inherent IACs properties such as cross-reactivity, analytes’ competition and capacity. The method was applied to determine DON and its major conjugates in barley, wheat and maize in the range of 10–1000 µg kg<sup>−1</sup> of DON, 10–300 µg kg<sup>−1</sup> of DON-3-G and 15-AcDON and 10–100 µg kg<sup>−1</sup> of 3-AcDON. The apparent recoveries varied from 87% to 110% (average of 98%) and the intermediate precision was below 13.5% RSD (except for DON-3-G in wheat). Fifteen maize, wheat and barley samples were analysed revealing levels of DON conjugates that accounted from 9% to 60% of the “total DON” content (m/m). In general, the frequency and the measured mass fractions decreased in the following order: DON&gt;DON-3-G&gt;15-AcDON&gt;3-AcDON.
DOI: 10.6084/m9.figshare.12853898.v2
title: Development and characterisation of a monoclonal antibody to detect the mycotoxin roquefortine C

description: Roquefortine, also known as roquefortine C (ROQC) is a fungal secondary metabolite (mycotoxin) that is produced by some of the same Penicillia as the tremorgen penitrem-A (PEN-A). The two mycotoxins have been linked to sporadic cases of toxicosis in dogs, cattle, and humans, leading some to consider ROQC as a biomarker of PEN-A. Reported here are the development of a monoclonal antibody (mAb) and associated competitive enzyme-linked immunosorbent assay (ELISA) for the screening of ROQC in extracts of nuts (nut “milks”), and dog serum. The ELISA was sensitive for ROQC, with a level of 0.117 ng ml<sup>−1</sup> inhibiting colour development by 50% (IC<sub>50</sub>), a limit of detection of 0.026 ng ml<sup>−1</sup>, and a dynamic range (IC<sub>20</sub> to IC<sub>80</sub>) of 0.038 to 0.289 ng ml<sup>−1</sup> in buffer. The assay was tolerant to significant levels of methanol. Recoveries from 4 types of nut milks spiked over the range of 0.25 to 2 ng ml<sup>−1</sup> were in the range of 83.5% to 116%. A small survey of commercial nut “milks” and “creamers” indicated 4 of 35 samples contained ROQC at levels so low that they are unlikely to be significant to human health (&lt;0.6 ng ml<sup>−1</sup>). The assay was also applied to canine serum. Recoveries from serum spiked over the range of 0.2 to 5 ng ml<sup>−1</sup> ranged from 98.1% to 123%. The results suggest the ELISA can be applied to the screening of food products, such as nut extracts, as well as for the screening of serum from dogs suspected to be suffering from mycotoxin-induced tremors.
DOI: 10.6084/m9.figshare.12902698
title: Determination of deoxynivalenol and its major conjugates in cereals using an organic solvent-free extraction and IAC clean-up coupled in-line with HPLC-PCD-FLD

description: A method for the determination of deoxynivalenol (DON) and its major conjugates in cereals was developed including an immunoaffinity column (IAC) clean-up coupled in-line with high-performance liquid chromatography, post-column derivatisation and fluorescence detection. An IAC for DON with cross-reactivity to 15-AcDON, 3-AcDON and DON-3-G enabled this approach. The isolated analytes were introduced into the chromatographic system without aliquotation employing the hot water elution technique, resulting in the desired low LOQ values for monitoring these analytes in cereals. The absence of any organic solvent during sample preparation in combination with an in-line IAC clean-up renders the method simple, fast, and environmentally friendly. Special attention was paid to inherent IACs properties such as cross-reactivity, analytes’ competition and capacity. The method was applied to determine DON and its major conjugates in barley, wheat and maize in the range of 10–1000 µg kg<sup>−1</sup> of DON, 10–300 µg kg<sup>−1</sup> of DON-3-G and 15-AcDON and 10–100 µg kg<sup>−1</sup> of 3-AcDON. The apparent recoveries varied from 87% to 110% (average of 98%) and the intermediate precision was below 13.5% RSD (except for DON-3-G in wheat). Fifteen maize, wheat and barley samples were analysed revealing levels of DON conjugates that accounted from 9% to 60% of the “total DON” content (m/m). In general, the frequency and the measured mass fractions decreased in the following order: DON&gt;DON-3-G&gt;15-AcDON&gt;3-AcDON.
DOI: 10.6084/m9.figshare.12853904
title: Ochratoxin A presence in Cabernet Sauvignon wine changes antioxidant activity <i>in vitro</i> and oxidative stress markers <i>in vivo</i>

description: Ochratoxin A (OTA) is a mycotoxin found in grape products and oxidative stress has been reported as an important mechanism involved in its toxicity, classified as possible carcinogenic to humans. Conversely, phenolics are known bioactive compounds in grapes and display great antioxidant properties. However, the biological effects of the concomitant presence of phenolic compounds and OTA remains unclear. The aim of this study was to evaluate, for the first time, the effect of OTA presence in Cabernet Sauvignon wine on antioxidant activity <i>in vitro</i> and on oxidative stress markers <i>in vivo</i>. In addition, the phenolic composition of wine was evaluated by LC-DAD-MS/MS. <i>In vitro</i> assays were based on spectrophotometric methods, while i<i>n vivo</i> assays were performed evaluating oxidative stress markers in the nematode <i>Caenorhabditis elegans</i>, an alternative model to animal testing. A total of 23 phenolic compounds were identified in the Cabernet sauvignon red wine, including the anthocyanins delphinidin-3-<i>O</i>-glicoside and malvidin-3-<i>O</i>-glicoside, the flavonol quercetin-3-<i>O</i>-glucuronide and the phenolic acids caffeic, verbascoside and caftaric. <i>Trans</i>-resveratrol and <i>trans</i>-piceid were the only stilbenes found in the samples. OTA presence in the red wine was accompanied by reduction in GSH content and increase in hydroxyl radical generation <i>in vitro</i>. The presence of OTA in wine also increased lipoperoxidation and induced overexpression of the antioxidant enzymes superoxide dismutase and catalase <i>in vivo</i>. This study demonstrates that OTA presence in red wine can reduce its antioxidant potential <i>in vitro</i> and induces oxidative stress <i>in vivo</i>, without affecting the phenolic compounds levels in the samples. Thus, this work provides insights into the negative effects of the presence of OTA in wine, not only by its known toxicity, but also by prejudicing the antioxidant potential of wine. It is important to be aware of these effects when developing a complete description of OTA toxicity in humans.
DOI: 10.6084/m9.figshare.12853904.v1
title: Ochratoxin A presence in Cabernet Sauvignon wine changes antioxidant activity <i>in vitro</i> and oxidative stress markers <i>in vivo</i>

description: Ochratoxin A (OTA) is a mycotoxin found in grape products and oxidative stress has been reported as an important mechanism involved in its toxicity, classified as possible carcinogenic to humans. Conversely, phenolics are known bioactive compounds in grapes and display great antioxidant properties. However, the biological effects of the concomitant presence of phenolic compounds and OTA remains unclear. The aim of this study was to evaluate, for the first time, the effect of OTA presence in Cabernet Sauvignon wine on antioxidant activity <i>in vitro</i> and on oxidative stress markers <i>in vivo</i>. In addition, the phenolic composition of wine was evaluated by LC-DAD-MS/MS. <i>In vitro</i> assays were based on spectrophotometric methods, while i<i>n vivo</i> assays were performed evaluating oxidative stress markers in the nematode <i>Caenorhabditis elegans</i>, an alternative model to animal testing. A total of 23 phenolic compounds were identified in the Cabernet sauvignon red wine, including the anthocyanins delphinidin-3-<i>O</i>-glicoside and malvidin-3-<i>O</i>-glicoside, the flavonol quercetin-3-<i>O</i>-glucuronide and the phenolic acids caffeic, verbascoside and caftaric. <i>Trans</i>-resveratrol and <i>trans</i>-piceid were the only stilbenes found in the samples. OTA presence in the red wine was accompanied by reduction in GSH content and increase in hydroxyl radical generation <i>in vitro</i>. The presence of OTA in wine also increased lipoperoxidation and induced overexpression of the antioxidant enzymes superoxide dismutase and catalase <i>in vivo</i>. This study demonstrates that OTA presence in red wine can reduce its antioxidant potential <i>in vitro</i> and induces oxidative stress <i>in vivo</i>, without affecting the phenolic compounds levels in the samples. Thus, this work provides insights into the negative effects of the presence of OTA in wine, not only by its known toxicity, but also by prejudicing the antioxidant potential of wine. It is important to be aware of these effects when developing a complete description of OTA toxicity in humans.
DOI: 10.6084/m9.figshare.15798231
title: datasets for "Within-corridor heterogeneity more important than corridor design for maintaining butterfly functional and taxonomic diversity"

description: Datasets<br>
DOI: 10.6084/m9.figshare.12853898.v1
title: Development and characterisation of a monoclonal antibody to detect the mycotoxin roquefortine C

description: Roquefortine, also known as roquefortine C (ROQC) is a fungal secondary metabolite (mycotoxin) that is produced by some of the same Penicillia as the tremorgen penitrem-A (PEN-A). The two mycotoxins have been linked to sporadic cases of toxicosis in dogs, cattle, and humans, leading some to consider ROQC as a biomarker of PEN-A. Reported here are the development of a monoclonal antibody (mAb) and associated competitive enzyme-linked immunosorbent assay (ELISA) for the screening of ROQC in extracts of nuts (nut “milks”), and dog serum. The ELISA was sensitive for ROQC, with a level of 0.117 ng ml<sup>−1</sup> inhibiting colour development by 50% (IC<sub>50</sub>), a limit of detection of 0.026 ng ml<sup>−1</sup>, and a dynamic range (IC<sub>20</sub> to IC<sub>80</sub>) of 0.038 to 0.289 ng ml<sup>−1</sup> in buffer. The assay was tolerant to significant levels of methanol. Recoveries from 4 types of nut milks spiked over the range of 0.25 to 2 ng ml<sup>−1</sup> were in the range of 83.5% to 116%. A small survey of commercial nut “milks” and “creamers” indicated 4 of 35 samples contained ROQC at levels so low that they are unlikely to be significant to human health (&lt;0.6 ng ml<sup>−1</sup>). The assay was also applied to canine serum. Recoveries from serum spiked over the range of 0.2 to 5 ng ml<sup>−1</sup> ranged from 98.1% to 123%. The results suggest the ELISA can be applied to the screening of food products, such as nut extracts, as well as for the screening of serum from dogs suspected to be suffering from mycotoxin-induced tremors.
DOI: 10.6084/m9.figshare.14417471
title: Influences of climate fluctuations on eastern Canada’s burned areas largely outweigh those of Euro-Canadian settlement since AD 1850

description: Data and R code for the paper : Influences of climate fluctuations on eastern Canada’s burned areas largely outweigh those of Euro-Canadian settlement since AD 1850(1850 to 1990)
DOI: 10.6084/m9.figshare.14417471.v2
title: Influences of climate fluctuations on eastern Canada’s burned areas largely outweigh those of Euro-Canadian settlement since AD 1850

description: Data and R code for the paper : Influences of climate fluctuations on eastern Canada’s burned areas largely outweigh those of Euro-Canadian settlement since AD 1850(1850 to 1990)
DOI: 10.6084/m9.figshare.15830523
title: simul_multiuav_sensors-1293532.xls

description: Data obtained after simulation of the different navigation algorithms presented in the manuscript MDPI sensors-1293532
DOI: 10.6084/m9.figshare.15830523.v1
title: simul_multiuav_sensors-1293532.xls

description: Data obtained after simulation of the different navigation algorithms presented in the manuscript MDPI sensors-1293532
DOI: 10.6084/m9.figshare.15015078.v1
title: Raw data on Speleomantes italicus growth

description: The CSV file includes: individual ID, population code (according to Lunghi et al. 2020, Scientific Data), indication on whether the surveyed Site was subterranean or epigean, Region and Province of the population location, Sex of the salamander (Juvenile, Male, Female), Year and Season of first capture (Year_F and Season_F), snout-vent length (SVL_F) and total length (TL_F) at first capture, Year and Season of last recapture (Year_L and Season_L), snout-vent length (SVL_L) and total length (TL_L) at last recapture, the number of seasons between the first and the last capture event (Season_gap), the increase of SVL (SVL_growth) and TL (TL growth) occurred between the first and the last capture event. The column “Used” indicates whether the data has been employed in analyses. All measurements of SVL and TL are shown in mm. NA means not available data.
DOI: 10.6084/m9.figshare.15830565.v1
title: simul_multiuav_sensors-1293532.xlsx

description: Datos obtenidos tras la simulación de los diferentes algoritmos de navegación presentados en el manuscrito MDPI sensors-1293532
DOI: 10.5517/ccdc.csd.cc245tkq
title: CCDC 1971803: Experimental Crystal Structure Determination

description: Related Article: Anna Pachariyangkun, Masayuki Suda, Sarinya Hadsadee, Siriporn Jungsuttiwong, Phattananawee Nalaoh, Pichaya Pattanasattayavong, Taweesak Sudyoadsuk, Hiroshi M. Yamamoto, Vinich Promarak|2020|J.Mater.Chem.C|8|17297|doi:10.1039/D0TC04982D
DOI: 10.5517/ccdc.csd.cc25djy2
title: CCDC 2008054: Experimental Crystal Structure Determination

description: Related Article: Anna Pachariyangkun, Masayuki Suda, Sarinya Hadsadee, Siriporn Jungsuttiwong, Phattananawee Nalaoh, Pichaya Pattanasattayavong, Taweesak Sudyoadsuk, Hiroshi M. Yamamoto, Vinich Promarak|2020|J.Mater.Chem.C|8|17297|doi:10.1039/D0TC04982D
DOI: 10.5517/ccdc.csd.cc27zc6t
title: CCDC 2084756: Experimental Crystal Structure Determination

description: Related Article: Changho Yoo, Alexander J. M. Miller|2021|J.Am.Chem.Soc.|143|12633|doi:10.1021/jacs.1c05185
DOI: 10.5517/ccdc.csd.cc27zc7v
title: CCDC 2084757: Experimental Crystal Structure Determination

description: Related Article: Changho Yoo, Alexander J. M. Miller|2021|J.Am.Chem.Soc.|143|12633|doi:10.1021/jacs.1c05185
DOI: 10.5517/ccdc.csd.cc2808f0
title: CCDC 2085631: Experimental Crystal Structure Determination

description: Related Article: Krista P. Balto, Milan Gembicky, Arnold L. Rheingold, Joshua S. Figueroa|2021|Inorg.Chem.|60|12545|doi:10.1021/acs.inorgchem.1c01804
DOI: 10.5517/ccdc.csd.cc2808g1
title: CCDC 2085632: Experimental Crystal Structure Determination

description: Related Article: Krista P. Balto, Milan Gembicky, Arnold L. Rheingold, Joshua S. Figueroa|2021|Inorg.Chem.|60|12545|doi:10.1021/acs.inorgchem.1c01804
DOI: 10.5517/ccdc.csd.cc2808h2
title: CCDC 2085633: Experimental Crystal Structure Determination

description: Related Article: Krista P. Balto, Milan Gembicky, Arnold L. Rheingold, Joshua S. Figueroa|2021|Inorg.Chem.|60|12545|doi:10.1021/acs.inorgchem.1c01804
DOI: 10.5517/ccdc.csd.cc280bj5
title: CCDC 2085696: Experimental Crystal Structure Determination

description: Related Article: Tomislav Krolo, Aditya Bhattacharyya, Oliver Reiser|2021|Org.Lett.|23|6283|doi:10.1021/acs.orglett.1c01964
DOI: 10.6084/m9.figshare.14459578.v1
title: Exploring the effectiveness of a digital voice assistant to maintain driver alertness in partially automated vehicles

description: Vehicle automation shifts the driver's role from active operator to passive observer at the potential cost of degrading their alertness. This study investigated the role of an in-vehicle voice-based assistant (VA; conversing about traffic/road environment) to counter the disengaging and fatiguing effects of automation. Twenty-four participants undertook two drives– with and without VA in a partially automated vehicle. Participants were subsequently categorized into high and low participation groups (based on their proportion of vocal exchanges with VA). The effectiveness of VA was assessed based on driver alertness measured using Karolinska Sleepiness Scale (KSS), eye-based sleepiness indicators and glance behavior, NASA-TLX workload rating and time to gain motor readiness in response to take-over request and performance rating made by the drivers. Paired samples <i>t-</i>tests comparison of alertness measures across the two drives were conducted. Lower KSS rating, larger pupil diameter, higher glances (rear-mirror, roadside vehicles and signals in the drive with VA) and higher feedback ratings of VA indicated the efficiency of VA in improving driver alertness during automation. However, there was no significant difference in alertness or glance behavior between the driver groups (high and low-PR), although the time to resume steering control was significantly lower in the higher engagement group. The study successfully demonstrated the advantages of using a voice assistant (VA) to counter these effects of passive fatigue, for example, by reducing the time to gain motor-readiness following a TOR. The findings show that despite the low engagement in spoken conversation, active listening also positively influenced driver alertness and awareness during the drive in an automated vehicle.
DOI: 10.6084/m9.figshare.14459578
title: Exploring the effectiveness of a digital voice assistant to maintain driver alertness in partially automated vehicles

description: Vehicle automation shifts the driver's role from active operator to passive observer at the potential cost of degrading their alertness. This study investigated the role of an in-vehicle voice-based assistant (VA; conversing about traffic/road environment) to counter the disengaging and fatiguing effects of automation. Twenty-four participants undertook two drives– with and without VA in a partially automated vehicle. Participants were subsequently categorized into high and low participation groups (based on their proportion of vocal exchanges with VA). The effectiveness of VA was assessed based on driver alertness measured using Karolinska Sleepiness Scale (KSS), eye-based sleepiness indicators and glance behavior, NASA-TLX workload rating and time to gain motor readiness in response to take-over request and performance rating made by the drivers. Paired samples <i>t-</i>tests comparison of alertness measures across the two drives were conducted. Lower KSS rating, larger pupil diameter, higher glances (rear-mirror, roadside vehicles and signals in the drive with VA) and higher feedback ratings of VA indicated the efficiency of VA in improving driver alertness during automation. However, there was no significant difference in alertness or glance behavior between the driver groups (high and low-PR), although the time to resume steering control was significantly lower in the higher engagement group. The study successfully demonstrated the advantages of using a voice assistant (VA) to counter these effects of passive fatigue, for example, by reducing the time to gain motor-readiness following a TOR. The findings show that despite the low engagement in spoken conversation, active listening also positively influenced driver alertness and awareness during the drive in an automated vehicle.