DOI: 10.5061/dryad.v5012
title: Data from: Fitness decline in spontaneous mutation accumulation lines of Caenorhabditis elegans with varying effective population sizes

description: The rate and fitness effects of new mutations have been investigated by
 mutation accumulation (MA) experiments in which organisms are maintained
 at a constant minimal population size to facilitate the accumulation of
 mutations with minimal efficacy of selection. We evolved 35 MA lines of
 Caenorhabditis elegans in parallel for 409 generations at three population
 sizes (N = 1, 10, and 100), representing the first spontaneous long-term
 MA experiment at varying population sizes with corresponding differences
 in the efficacy of selection. Productivity and survivorship in the N = 1
 lines declined by 44% and 12%, respectively. The average effects of
 deleterious mutations in N = 1 lines are estimated to be 16.4% for
 productivity and 11.8% for survivorship. Larger populations (N = 10 and
 100) did not suffer a significant decline in fitness traits despite a
 lengthy and sustained regime of consecutive bottlenecks exceeding 400
 generations. Together, these results suggest that fitness decline in very
 small populations is dominated by mutations with large deleterious
 effects. It is possible that the MA lines at larger population sizes
 contain a load of cryptic deleterious mutations of small to moderate
 effects that would be revealed in more challenging environments.
description: Mutation Accumulation
 Generation 100 Fitness DataProductivity and
 survivorship to adulthood for MA lines following 100 consecutive
 generations of mutation accumulation and the ancestral controls. Column 1
 corresponds to the line identifier (MA line or ancestral control line).
 Column 2 lists the replicate number for each line (where possible, we
 aimed for five replicates within each line). Columns 3-10 list the worm
 productivity corresponding to Days 1-8 of the productivity assay. Column
 11 is the total productivity summed across Days 1-8. Column 12 lists the
 number of surviving adults from a pool of L1 larvae initially isolated.
 Column 13 is the final value for survivorship to adulthood and ranges from
 0 to 1 (corresponding to 0 to 100% survivorship to adulthood). Dashes in
 cells represent lack of data due to hermaphrodite mortality during the
 assay or inability to enter the replicate line into assay due to sterility
 or mortality in preceding generations.MA_Gen_100(FitnessAssay)_Dryad.pdfMutation Accumulation Generation 172 Fitness DataProductivity and survivorship to adulthood for MA lines following 172 consecutive generations of mutation accumulation and the ancestral controls. Column 1 corresponds to the line identifier (MA line or ancestral control line). Column 2 lists the replicate number for each line (where possible, we aimed for five replicates within each line). Columns 3-10 list the worm productivity corresponding to Days 1-8 of the productivity assay. Column 11 is the total productivity summed across Days 1-8. Column 12 lists the number of surviving adults from a pool of L1 larvae initially isolated. Column 13 is the final value for survivorship to adulthood and ranges from 0 to 1 (corresponding to 0 to 100% survivorship to adulthood). Dashes in cells represent lack of data due to hermaphrodite mortality during the assay or inability to enter the replicate line into assay due to sterility or mortality in preceding generations.MA_Gen_172(FitnessAssay)_Dryad.pdfMutation Accumulation Generation 300 Fitness DataProductivity and survivorship to adulthood for MA lines following 300 consecutive generations of mutation accumulation and the ancestral controls. Column 1 corresponds to the line identifier (MA line or ancestral control line). Column 2 lists the replicate number for each line (where possible, we aimed for five replicates within each line). Columns 3-10 list the worm productivity corresponding to Days 1-8 of the productivity assay. Column 11 is the total productivity summed across Days 1-8. Column 12 lists the number of surviving adults from a pool of L1 larvae initially isolated. Column 13 is the final value for survivorship to adulthood and ranges from 0 to 1 (corresponding to 0 to 100% survivorship to adulthood). Dashes in cells represent lack of data due to hermaphrodite mortality during the assay or inability to enter the replicate line into assay due to sterility or mortality in preceding generations.MA_Gen_300(FitnessAssay)_Dryad.pdfMutation Accumulation Generation 409 Fitness DataProductivity and survivorship to adulthood for MA lines following 409 consecutive generations of mutation accumulation and the ancestral controls. Column 1 corresponds to the line identifier (MA line or ancestral control line). Column 2 lists the replicate number for each line (where possible, we aimed for five replicates within each line). Columns 3-10 list the worm productivity corresponding to Days 1-8 of the productivity assay. Column 11 is the total productivity summed across Days 1-8. Column 12 lists the number of surviving adults from a pool of L1 larvae initially isolated. Column 13 is the final value for survivorship to adulthood and ranges from 0 to 1 (corresponding to 0 to 100% survivorship to adulthood). Dashes in cells represent lack of data due to hermaphrodite mortality during the assay or inability to enter the replicate line into assay due to sterility or mortality in preceding generations.MA_Gen_409(FitnessAssay)_Dryad.pdf