cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11452530
title: Thalidomide Reduces Cell Proliferation in Endometriosis Experimentally Induced in Rats

description: Abstract Objective To analyze the effect of thalidomide on the progression of endometriotic lesions experimentally induced in rats and to characterize the pattern of cell proliferation by immunohistochemical Proliferating Cell Nuclear Antigen (PCNA) labeling of eutopic and ectopic endometrium. Methods Fifteen female Wistar rats underwent laparotomy for endometriosis induction by resection of one uterine horn, isolation of the endometrium and fixation of a tissue segment to the pelvic peritoneum. Four weeks after, the animals were divided into 3 groups: control (I), 10mg/kg/day (II) and 1mg/kg/day (III) intraperitoneal thalidomide for 10 days. The lesion was excised together with the opposite uterine horn for endometrial gland and stroma analysis. Eutopic and ectopic endometrial tissue was submitted to immunohistochemistry for analysis of cell proliferation by PCNA labeling and the cell proliferation index (CPI) was calculated as the number of labeled cells per 1,000 cells. Results Group I showed a mean CPI of 0.248 ± 0.0513 in the gland and of 0.178 ± 0.046 in the stroma. In contrast, Groups II and III showed a significantly lower CPI, that is, 0.088 ± 0.009 and 0.080 ± 0.021 for the gland (p &lt; 0.001) and 0.0945 ± 0.0066 and 0.075 ± 0.018 for the stroma (p &lt; 0.001), respectively. Also, the mean lesion area of Group I was 69.2mm2, a significantly higher value compared with Group II (49.4mm2, p = 0.023) and Group III (48.6mm2, p = 0.006). No significant difference was observed between Groups II and III. Conclusion Thalidomide proved to be effective in reducing the lesion area and CPI of the experimental endometriosis implants both at the dose of 1mg/kg/day and at the dose of 10 mg/kg/day.
DOI: 10.6084/m9.figshare.11452527.v1
title: Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways

description: Total Panax notoginseng saponin (TPNS) is the main bioactivity compound derived from the roots and rhizomes of Panax notoginseng (Burk.) F.H. Chen. The aim of this study was to investigate the effectiveness of TPNS in treating vascular neointimal hyperplasia in rats and its mechanisms. Male Sprague-Dawley rats were randomly divided into five groups, sham (control), injury, and low, medium, and high dose TPNS (5, 10, and 20 mg/kg). An in vivo 2F Fogarty balloon-induced carotid artery injury model was established in rats. TPNS significantly and dose-dependently reduced balloon injury-induced neointimal area (NIA) (P&lt;0.001, for all doses) and NIA/media area (MA) (P&lt;0.030, for all doses) in the carotid artery of rats, and PCNA expression (P&lt;0.001, all). The mRNA expression of smooth muscle (SM) α-actin was significantly increased in all TPNS groups (P&lt;0.005, for all doses) and the protein expression was significantly increased in the medium (P=0.006) and high dose TPNS (P=0.002) groups compared to the injury group. All the TPNS doses significantly decreased the mRNA expression of c-fos (P&lt;0.001). The medium and high dose TPNS groups significantly suppressed the upregulation of pERK1/2 protein in the NIA (P&lt;0.025) and MA (P&lt;0.004). TPNS dose-dependently inhibited balloon injury-induced activation of pERK/p38MAPK signaling in the carotid artery. TPNS could be a promising agent in inhibiting cell proliferation following vascular injuries.
DOI: 10.6084/m9.figshare.11452530.v1
title: Thalidomide Reduces Cell Proliferation in Endometriosis Experimentally Induced in Rats

description: Abstract Objective To analyze the effect of thalidomide on the progression of endometriotic lesions experimentally induced in rats and to characterize the pattern of cell proliferation by immunohistochemical Proliferating Cell Nuclear Antigen (PCNA) labeling of eutopic and ectopic endometrium. Methods Fifteen female Wistar rats underwent laparotomy for endometriosis induction by resection of one uterine horn, isolation of the endometrium and fixation of a tissue segment to the pelvic peritoneum. Four weeks after, the animals were divided into 3 groups: control (I), 10mg/kg/day (II) and 1mg/kg/day (III) intraperitoneal thalidomide for 10 days. The lesion was excised together with the opposite uterine horn for endometrial gland and stroma analysis. Eutopic and ectopic endometrial tissue was submitted to immunohistochemistry for analysis of cell proliferation by PCNA labeling and the cell proliferation index (CPI) was calculated as the number of labeled cells per 1,000 cells. Results Group I showed a mean CPI of 0.248 ± 0.0513 in the gland and of 0.178 ± 0.046 in the stroma. In contrast, Groups II and III showed a significantly lower CPI, that is, 0.088 ± 0.009 and 0.080 ± 0.021 for the gland (p &lt; 0.001) and 0.0945 ± 0.0066 and 0.075 ± 0.018 for the stroma (p &lt; 0.001), respectively. Also, the mean lesion area of Group I was 69.2mm2, a significantly higher value compared with Group II (49.4mm2, p = 0.023) and Group III (48.6mm2, p = 0.006). No significant difference was observed between Groups II and III. Conclusion Thalidomide proved to be effective in reducing the lesion area and CPI of the experimental endometriosis implants both at the dose of 1mg/kg/day and at the dose of 10 mg/kg/day.
DOI: 10.6084/m9.figshare.11452524.v1
title: Use of flash glucose-sensing technology in patients with type 2 diabetes treated with liraglutide combined with CSII: a pilot study

description: Glycemic variability (GV) may be linked to the development of diabetic complications by inducing inflammation, oxidative stress, and endothelial dysfunction. Flash glucose monitoring (FGM) provides a novel method of continuously monitoring interstitial glucose levels for up to 14 days. This study randomly assigned poorly controlled type 2 diabetes mellitus patients treated with metformin and multiple daily injections of insulin (n=60) to either continuous subcutaneous insulin infusion (CSII) treatment or CSII in combination with liraglutide (CSII+Lira) treatment for 14 days during hospitalization. GV was assessed using a FGM system; weight and cardiometabolic biomarkers were also evaluated. The coefficient of variation was significantly reduced in the CSII+Lira group (P&lt;0.001), while no significant change was observed in the CSII group. The changes differed significantly between the two groups in mean amplitude of glycemic excursions (P=0.004), standard deviation (P=0.006), and the percentage of time in the target range (4–10 mmol/L, P=0.005 and &gt;10 mmol/L, P=0.028). The changes in mean of daily differences, interquartile range, and percentage of time in hypoglycemia (&lt;3.3 mmol/L) and hyperglycemia (&gt;13.9 mmol/L) identified by FGM showed no difference. Treatment with liraglutide increased serum adiponectin [33.5 (3.5, 47.7) pg/mL, P=0.003] and heme oxygenase-1 levels [0.4 (–0.0, 1.8) ng/mL, P=0.001] and reduced serum leptin levels [–2.8 (3.9) pg/mL, P&lt;0.001]. Adding the glucagon-like peptide-1 analog liraglutide improved GV, weight, and some cardiometabolic risk markers. The FGM system is, therefore, shown to be a novel and useful method for glucose monitoring.
DOI: 10.6084/m9.figshare.11452515
title: CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice

description: Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine hypertrophy, inhibiting edema formation. Finally, histological analysis of the orcein-stained uteruses showed that the antagonist reduced eosinophil migration together with edema. These data showed that the CCR3 receptor is an important target for studies that seek to clarify the functions of these cells in uterine physiology.
DOI: 10.6084/m9.figshare.11452512
title: DROP HEIGHT IS INFLUENCED BY BOX HEIGHT BUT NOT BY INDIVIDUAL STATURE DURING DROP JUMPS

description: ABSTRACT Drop jump (DJ) performance an important aspect in many sports. The optimal stretch load in the DJ training is commonly prescribed through the box height from which individuals must drop from. However, studies have reported differences between real (calculated) drop heights and box heights, which may affect the quality of the prescription of DJ training load. Other aspects such as individual stature may also affect the differences between calculated drop heights and box heights. This study aimed to 1) investigate the difference between calculated drop heights and box heights in different box heights and 2) verify the influence of individual stature on the difference between calculated drop heights and box heights. Twenty-two individuals performed DJs from different box heights and landed on a force platform. An ANCOVA was performed to verify the influence of stature and box height on the difference between calculated drop heights and box heights. We found that calculated drop height was different from box height for all box heigths. The difference between calculated drop height and box height significantly increased as box heights increased. Individual stature did not influence the difference between calculated drop heights and box heights. We can concluded that box heights are significantly different from calculated drop heights. These differences increase as the box height increases, i.e. an increase in the difference of approximately 2 cm every 10 cm increase in box height. Therefore, it seems that coaches can use the same procedures for prescribing DJ training load for individuals of different heights
DOI: 10.6084/m9.figshare.11452515.v1
title: CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice

description: Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine hypertrophy, inhibiting edema formation. Finally, histological analysis of the orcein-stained uteruses showed that the antagonist reduced eosinophil migration together with edema. These data showed that the CCR3 receptor is an important target for studies that seek to clarify the functions of these cells in uterine physiology.
DOI: 10.6084/m9.figshare.11452512.v1
title: DROP HEIGHT IS INFLUENCED BY BOX HEIGHT BUT NOT BY INDIVIDUAL STATURE DURING DROP JUMPS

description: ABSTRACT Drop jump (DJ) performance an important aspect in many sports. The optimal stretch load in the DJ training is commonly prescribed through the box height from which individuals must drop from. However, studies have reported differences between real (calculated) drop heights and box heights, which may affect the quality of the prescription of DJ training load. Other aspects such as individual stature may also affect the differences between calculated drop heights and box heights. This study aimed to 1) investigate the difference between calculated drop heights and box heights in different box heights and 2) verify the influence of individual stature on the difference between calculated drop heights and box heights. Twenty-two individuals performed DJs from different box heights and landed on a force platform. An ANCOVA was performed to verify the influence of stature and box height on the difference between calculated drop heights and box heights. We found that calculated drop height was different from box height for all box heigths. The difference between calculated drop height and box height significantly increased as box heights increased. Individual stature did not influence the difference between calculated drop heights and box heights. We can concluded that box heights are significantly different from calculated drop heights. These differences increase as the box height increases, i.e. an increase in the difference of approximately 2 cm every 10 cm increase in box height. Therefore, it seems that coaches can use the same procedures for prescribing DJ training load for individuals of different heights
DOI: 10.6084/m9.figshare.11452485
title: Factors affecting fluoroscopy time during percutaneous nephrolithotomy: Impact of stone volume distribution in renal collecting system

description: ABSTRACT Purpose: To identify the factors increased fluoroscopy time during percutaneous nephrolithotomy and investigate the relationship between the 3D segmentation volume ratio of stone to renal collecting system and fluoroscopy time. Materials and Methods: Data from 102 patients who underwent percutaneous nephrolithotomy were analyzed retrospectively. Volume segmentation of both the renal collecting system and stones were obtained from 3D segmentation software with the images on CT data. Analyzed stone volume (ASV), renal collecting system volume (RCSV) measured and the ASV-to-RCSV ratio was calculated. Several parameters were evaluated for their predictive ability with regard to fluoroscopy time. Results: The stone-free rate was 55.9% after the percutaneous nephrolithotomy. Complications occurred in 31(30.4%) patients. The mean fluoroscopy time was 199.4±151.1 seconds. The fluoroscopy time was significantly associated with the ASV-to-RCSV ratio (p&lt;0.001, r=0.614). The single tract was used in 77 (75.5%) cases while multiple tracts were used in 25 (24.5%) cases. Fluoroscopy time was significantly associated with multiple access (p&lt;0.001, r=0.689). On univariate linear regression analysis, longer fluoroscopy time was related with increased stone size, increased stone volume, increased number of access, increased calyx number with stone, increased ASV-to-RCSV, increased operative time and decreased stone essence. On multivariate linear regression analysis, the number of access and the ASV-to-RCSV were independent predictors of fluoroscopy time during percutaneous nephrolithotomy. Conclusions: The distribution of the stone burden volume in the pelvicalyceal system is a significant predictor for prolonged fluoroscopy time during percutaneous nephrolithotomy. Measures to decrease FT could be beneficial in patients with a high ASV-to-RCSV ratio for precise preoperative planning.
DOI: 10.6084/m9.figshare.11452488
title: The study of the historiographies by Paul Karlson, Konstantin Ríbnikov, Howard Eves and Bento de Jesus Caraça: different ways of looking at and conceiving the concept of function

description: Abstract: This article aims to present some of the studies that have been done on the historiographies written by Paul Karlson, Konstantin Ríbnikov, Howard Eves and Bento de Jesus Caraça. The ways of looking at and conceiving the concept of function by those authors is underscored. These studies are part of theoretical research, characterized as documentary and qualitative. They consider that the historiographical aspects of the History of Mathematics describe the logic of infinite historical facts that occurred in diverse contexts, at certain times and spaces, which are systematized, from time to time. It is assumed that if the historiographies of Mathematics address the history of the knowledge of mathematical concepts, while studying them, especially those who teach mathematics can delineate some conceptual links (internal and external) that make up the concepts. In the case of the concept of function, the following are the nexuses: irregular and regular movements, interdependence, variation, field of variation, relations and representations.
DOI: 10.6084/m9.figshare.11452488.v1
title: The study of the historiographies by Paul Karlson, Konstantin Ríbnikov, Howard Eves and Bento de Jesus Caraça: different ways of looking at and conceiving the concept of function

description: Abstract: This article aims to present some of the studies that have been done on the historiographies written by Paul Karlson, Konstantin Ríbnikov, Howard Eves and Bento de Jesus Caraça. The ways of looking at and conceiving the concept of function by those authors is underscored. These studies are part of theoretical research, characterized as documentary and qualitative. They consider that the historiographical aspects of the History of Mathematics describe the logic of infinite historical facts that occurred in diverse contexts, at certain times and spaces, which are systematized, from time to time. It is assumed that if the historiographies of Mathematics address the history of the knowledge of mathematical concepts, while studying them, especially those who teach mathematics can delineate some conceptual links (internal and external) that make up the concepts. In the case of the concept of function, the following are the nexuses: irregular and regular movements, interdependence, variation, field of variation, relations and representations.
DOI: 10.6084/m9.figshare.11452482
title: Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice

description: Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.
DOI: 10.6084/m9.figshare.11452482.v1
title: Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice

description: Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.
DOI: 10.6084/m9.figshare.11452485.v1
title: Factors affecting fluoroscopy time during percutaneous nephrolithotomy: Impact of stone volume distribution in renal collecting system

description: ABSTRACT Purpose: To identify the factors increased fluoroscopy time during percutaneous nephrolithotomy and investigate the relationship between the 3D segmentation volume ratio of stone to renal collecting system and fluoroscopy time. Materials and Methods: Data from 102 patients who underwent percutaneous nephrolithotomy were analyzed retrospectively. Volume segmentation of both the renal collecting system and stones were obtained from 3D segmentation software with the images on CT data. Analyzed stone volume (ASV), renal collecting system volume (RCSV) measured and the ASV-to-RCSV ratio was calculated. Several parameters were evaluated for their predictive ability with regard to fluoroscopy time. Results: The stone-free rate was 55.9% after the percutaneous nephrolithotomy. Complications occurred in 31(30.4%) patients. The mean fluoroscopy time was 199.4±151.1 seconds. The fluoroscopy time was significantly associated with the ASV-to-RCSV ratio (p&lt;0.001, r=0.614). The single tract was used in 77 (75.5%) cases while multiple tracts were used in 25 (24.5%) cases. Fluoroscopy time was significantly associated with multiple access (p&lt;0.001, r=0.689). On univariate linear regression analysis, longer fluoroscopy time was related with increased stone size, increased stone volume, increased number of access, increased calyx number with stone, increased ASV-to-RCSV, increased operative time and decreased stone essence. On multivariate linear regression analysis, the number of access and the ASV-to-RCSV were independent predictors of fluoroscopy time during percutaneous nephrolithotomy. Conclusions: The distribution of the stone burden volume in the pelvicalyceal system is a significant predictor for prolonged fluoroscopy time during percutaneous nephrolithotomy. Measures to decrease FT could be beneficial in patients with a high ASV-to-RCSV ratio for precise preoperative planning.
DOI: 10.6084/m9.figshare.11452479
title: Scheduling optimum planting window for gladiola based on El Niño Southern Oscillation

description: ABSTRACT: Gladiola is an important cut flower worldwide and scheduling field gladiola production is largely dependent on planting at the correct time since its development is controlled by the air temperature. The aim of this study was to configure a planting window of gladiola in order to harvest spikes to be sold on All Souls' Day and Mother's Day holidays in the state of Rio Grande do Sul, Brazil, taking into account the El Niño Southern Oscillation (ENSO) phases. The PhenoGlad model, a dynamic process-based simulation model that simulates gladiola phenology on a daily time step was used in this study. Daily minimum and maximum temperatures over 55 years (1961-2015) from 18 weather stations across Rio Grande do Sul state, Brazil, were used as input data in the PhenoGlad model. The optimum planting window was simulated for each maturity group, location and ENSO phase. For harvesting on All Souls' Day, the optimum planting window had greater variation among the different ENSO phases. For Mother's Day, only Very Strong El Niño events affected the development of gladiola, indicating the weak signal of the phenomenon at this time of the year. A case study in a commercial farm demonstrated the importance of these results for farmers. The planting window for production on Mother's Day in a Neutral year was successfully configured. Determining the optimum planting window by considering the ENSO effect led to a more precise scheduling of gladiola production in southern Brazil.