cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.17353740
title: Spooner et al 2025 Dataset (10.1073/pnas.2413308122)

description: Abstract

The L-type Ca2+ channel (CaV1.2) is essential for cardiac excitation–contraction coupling. To contribute to the inward Ca2+ flux that drives Ca2+-induced-Ca2+-release, CaV1.2 channels must be expressed on the sarcolemma; thus the regulatory mechanisms that tune CaV1.2 expression to meet contractile demand are an emerging area of research. A ubiquitously expressed protein called 14-3-3 has been proposed to affect Ca2+ channel trafficking in nonmyocytes; however, whether 14-3-3 has similar effects on CaV1.2 in cardiomyocytes is unknown. 14-3-3 preferentially binds phospho-serine/threonine residues to affect many cellular processes and is known to regulate cardiac ion channels including NaV1.5 and the human ether-à-go-go–related gene (hERG) potassium channel. Altered 14-3-3 expression and function have been implicated in cardiac pathologies including hypertrophy. Accordingly, we tested the hypothesis that 14-3-3 interacts with CaV1.2 in a phosphorylation-dependent manner and regulates cardiac CaV1.2 trafficking and recycling. Confocal imaging, proximity ligation assays, superresolution imaging, and coimmunoprecipitation revealed a population of 14-3-3 colocalized and closely associated with CaV1.2. The degree of 14-3-3/CaV1.2 colocalization increased upon stimulation of β-adrenergic receptors with isoproterenol. Notably, only the 14-3-3-associated CaV1.2 population displayed increased cluster size with isoproterenol, revealing a role for 14-3-3 as a nucleation factor that directs CaV1.2 superclustering. Isoproterenol-stimulated augmentation of sarcolemmal CaV1.2 expression, Ca2+ currents, and Ca2+ transients in ventricular myocytes were strengthened by 14-3-3 overexpression and attenuated by 14-3-3 inhibition. These data support a model where 14-3-3 interacts with CaV1.2 in a phosphorylation-dependent manner to promote enhanced trafficking/recycling, clustering, and activity during β-adrenergic stimulation.
description: This repository contains the datasets and statistical analyses associated with the publication:

Spooner HC, Costa AD, Westhoff M, Hernández-González A, Ibrahimkhail H, Yarov-Yarovoy V, Horne MC, Dickson EJ, Dixon RE. 14-3-3 promotes sarcolemmal expression of cardiac CaV1.2 and nucleates isoproterenol-triggered channel superclustering. PNAS 2025. 122(5):e2413308122. doi: 10.1073/pnas.2413308122. PMCID: PMC11804677.

The data have been organized to ensure transparency and reproducibility. Each figure has an independent folder containing an Excel file with the data points and summary statistics.
DOI: 10.5281/zenodo.17353741
title: Spooner et al 2025 Dataset (10.1073/pnas.2413308122)

description: Abstract

The L-type Ca2+ channel (CaV1.2) is essential for cardiac excitation–contraction coupling. To contribute to the inward Ca2+ flux that drives Ca2+-induced-Ca2+-release, CaV1.2 channels must be expressed on the sarcolemma; thus the regulatory mechanisms that tune CaV1.2 expression to meet contractile demand are an emerging area of research. A ubiquitously expressed protein called 14-3-3 has been proposed to affect Ca2+ channel trafficking in nonmyocytes; however, whether 14-3-3 has similar effects on CaV1.2 in cardiomyocytes is unknown. 14-3-3 preferentially binds phospho-serine/threonine residues to affect many cellular processes and is known to regulate cardiac ion channels including NaV1.5 and the human ether-à-go-go–related gene (hERG) potassium channel. Altered 14-3-3 expression and function have been implicated in cardiac pathologies including hypertrophy. Accordingly, we tested the hypothesis that 14-3-3 interacts with CaV1.2 in a phosphorylation-dependent manner and regulates cardiac CaV1.2 trafficking and recycling. Confocal imaging, proximity ligation assays, superresolution imaging, and coimmunoprecipitation revealed a population of 14-3-3 colocalized and closely associated with CaV1.2. The degree of 14-3-3/CaV1.2 colocalization increased upon stimulation of β-adrenergic receptors with isoproterenol. Notably, only the 14-3-3-associated CaV1.2 population displayed increased cluster size with isoproterenol, revealing a role for 14-3-3 as a nucleation factor that directs CaV1.2 superclustering. Isoproterenol-stimulated augmentation of sarcolemmal CaV1.2 expression, Ca2+ currents, and Ca2+ transients in ventricular myocytes were strengthened by 14-3-3 overexpression and attenuated by 14-3-3 inhibition. These data support a model where 14-3-3 interacts with CaV1.2 in a phosphorylation-dependent manner to promote enhanced trafficking/recycling, clustering, and activity during β-adrenergic stimulation.
description: This repository contains the datasets and statistical analyses associated with the publication:

Spooner HC, Costa AD, Westhoff M, Hernández-González A, Ibrahimkhail H, Yarov-Yarovoy V, Horne MC, Dickson EJ, Dixon RE. 14-3-3 promotes sarcolemmal expression of cardiac CaV1.2 and nucleates isoproterenol-triggered channel superclustering. PNAS 2025. 122(5):e2413308122. doi: 10.1073/pnas.2413308122. PMCID: PMC11804677.

The data have been organized to ensure transparency and reproducibility. Each figure has an independent folder containing an Excel file with the data points and summary statistics.
DOI: 10.15468/dl.qh8tje
title: Occurrence Download

description: A dataset containing 225 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Begonia octopetala"
 ]
}

 The dataset includes 225 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020968-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.eq5bcy
title: Occurrence Download

description: A dataset containing 5477 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Salix amygdaloides)",
 "StateProvince is one of (Alberta, Arizona, British Columbia, Colorado, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Manitoba, Michigan, Minnesota, Missouri, Montana, Nebraska, Nevada, New Mexico, New York, North Dakota, Ohio, Oklahoma, Ontario, Oregon, Pennsylvania, Quebec, Saskatchewan, South Dakota, Texas, Utah, Vermont, Washington, Wisconsin, Wyoming)",
 "BasisOfRecord is Specimen"
 ]
}

 The dataset includes 5477 records from 159 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020969-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15547133
title: Temperature effects on reproductive and life-history traits in Drosophila: a systematic map

description: List of published studies that examined the effects of temperature on diverse fitness-related traits in Drosophila species. It focuses on six main reproductive traits: fertility, fecundity, mating traits, egg hatching, gamete traits, and gonad traits; and four key life-history traits: longevity, mortality, viability, and development time.
DOI: 10.5281/zenodo.17353764
title: Temperature effects on reproductive and life-history traits in Drosophila: a systematic map

description: List of published studies that examined the effects of temperature on diverse fitness-related traits in Drosophila species. It focuses on six main reproductive traits: fertility, fecundity, mating traits, egg hatching, gamete traits, and gonad traits; and four key life-history traits: longevity, mortality, viability, and development time.
DOI: 10.15468/dl.zw3wu2
title: Occurrence Download

description: A dataset containing 919 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is South Africa",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Dasispermum suffruticosum"
 ]
}

 The dataset includes 919 records from 11 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020970-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.v97gdp
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Gobio occitaniae",
 "Country is Spain",
 "HasCoordinate is true"
 ]
}

 The dataset includes 5 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020971-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.qyu5zg
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Piciformes)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((-50 40,-40 40,-40 50,-50 50,-50 40))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020974-251009101135966/datasets/export for details.
DOI: 10.15468/dl.7e99cz
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Mesitornithiformes)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((100 -90,110 -90,110 -80,100 -80,100 -90))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020975-251009101135966/datasets/export for details.
DOI: 10.15468/dl.hfnwe4
title: Occurrence Download

description: A dataset containing 561 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Disporopsis pernyi"
 ]
}

 The dataset includes 561 records from 38 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020972-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.56xqnb
title: Occurrence Download

description: A dataset containing 120 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Scaphopoda)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((20 -40,30 -40,30 -30,20 -30,20 -40))"
 ]
}

 The dataset includes 120 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020976-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/13386687
title: US counties COVID 19 dataset
DOI: 10.15468/dl.4b8jsf
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Polyplacophora)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((-180 70,-170 70,-170 80,-180 80,-180 70))"
 ]
}

 The dataset includes 8 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020978-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ddr82x
title: Occurrence Download

description: A dataset containing 9 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Scaphopoda)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((10 -40,20 -40,20 -30,10 -30,10 -40))"
 ]
}

 The dataset includes 9 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020977-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5517/cc8q2tv
title: CCDC 259557: Experimental Crystal Structure Determination

description: Related Article: Chun-Hui Mao, Hai-Bin Song, Qing-Min Wang, Run-Qiu Huang, Li Chen, Jian Shang|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2264|doi:10.1107/S1600536804028016
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q2vw
title: CCDC 259558: Experimental Crystal Structure Determination

description: Related Article: Fengke Yang, Hailian Xiao, Fangfang Jian|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2266|doi:10.1107/S1600536804027084
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q2wx
title: CCDC 259559: Experimental Crystal Structure Determination

description: Related Article: Jizhong Yan, Dongping Cheng|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2268|doi:10.1107/S1600536804026248
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q2xy
title: CCDC 259560: Experimental Crystal Structure Determination

description: Related Article: Hong-Bo Tong, Xue-Hong Wei, Dian-Sheng Liu, Shu-Ping Huang|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2270|doi:10.1107/S1600536804028065
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q2yz
title: CCDC 259561: Experimental Crystal Structure Determination

description: Related Article: D.P.Temelkoff, P.Norris, M.Zeller|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2273|doi:10.1107/S1600536804028405
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc806n3
title: CCDC 238534: Experimental Crystal Structure Determination

description: Related Article: Zheng-Wei Shi, Yi-Zhi Li, Ying Li, Guo-Yuan Lu, Shan-Hui Liu|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2275|doi:10.1107/S1600536804028168
description: Related Article: Zheng-Wei Shi, Ying Li, Guo-Yuan Lu|2005|Gaodeng Xuexiao Huaxue Xuebao|26|667|
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q2z0
title: CCDC 259562: Experimental Crystal Structure Determination

description: Related Article: Fangjun Huo, Caixia Yin, Pin Yang|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2278|doi:10.1107/S1600536804028478
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q302
title: CCDC 259563: Experimental Crystal Structure Determination

description: Related Article: S.O.Yildirim, M.Akkurt, M.Koparir, A.Cansiz, M.Sekerci, F.W.Heinemann|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2281|doi:10.1107/S1600536804028302
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q313
title: CCDC 259564: Experimental Crystal Structure Determination

description: Related Article: N.M.Comerlato, G.B.Ferreira, J.M.S.Skakle, J.L.Wardell|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2284|doi:10.1107/S1600536804028387
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q324
title: CCDC 259565: Experimental Crystal Structure Determination

description: Related Article: L.Mazur, A.E.Koziol, B.Modzelewska-Banachiewicz|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2287|doi:10.1107/S1600536804028417
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q335
title: CCDC 259566: Experimental Crystal Structure Determination

description: Related Article: K.Ravikumar, G.Y.S.K.Swamy, S.Raghavan, Ch.Naveenkumar|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2290|doi:10.1107/S1600536804028314
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q346
title: CCDC 259567: Experimental Crystal Structure Determination

description: Related Article: Xin-Gang Liu, Ya-Qing Feng, Peng Wu, Xue Chen, Fei Li|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2293|doi:10.1107/S1600536804028272
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q368
title: CCDC 259569: Experimental Crystal Structure Determination

description: Related Article: M.S.Datt, C.B.de Koning, M.A.Fernandes, J.P.Michael|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2298|doi:10.1107/S160053680402820X
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q379
title: CCDC 259570: Experimental Crystal Structure Determination

description: Related Article: T.Manifar, S.Rohani, M.C.Jennings|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2301|doi:10.1107/S1600536804028247
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/cc8q38b
title: CCDC 259571: Experimental Crystal Structure Determination

description: Related Article: J.W.Karban, J.L.Morgan, J.M.Dees, K.K.Klausmeyer|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2304|doi:10.1107/S1600536804028399
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.15468/dl.n8ku6m
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Mesitornithiformes)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((90 -90,100 -90,100 -80,90 -80,90 -90))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020979-251009101135966/datasets/export for details.
DOI: 10.5517/cc8q39c
title: CCDC 259572: Experimental Crystal Structure Determination

description: Related Article: N.E.Magana-Vergara, F.J.Martinez-Martinez, I.I.Padilla-Martinez, H.Hopfl, E.V.Garcia-Baez|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o2306|doi:10.1107/S160053680402851X
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.