cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.26008/1912/bco-dmo.960203.1
title: Presence and abundance of blue particles in Oceanic Flux Program (OFP) samples collected from 1984-2019 at the OFP mooring in the Sargasso Sea

description: These data are compiled from the Oceanic Flux Program (OFP) archive and show a number of OFP samples that contain blue particles along with the date of the collection period, depth, sample size fraction, and the presence/absence and abundance of the blue particles.
DOI: 10.26008/1912/bco-dmo.960257.1
title: Metadata (location, depth, water temperature, salinity, etc.) describing end members compared to blue particles found in Oceanic Flux Program (OFP) traps

description: This dataset includes metadata for end members (fish, bryzoan, serpulid worm, red algae, and coccolithophore) that were compared to blue particles found in OFP traps. Data include species, location, depth, water temperature, salinity, and date of collection.
DOI: 10.26008/1912/bco-dmo.751642.3
title: Habitat complexity photos collected along the Northern Central California coast from 2017 to 2021

description: Habitat complexity photos collected along the Northern Central California coast from 2017 to 2021.  GPS waypoints, quadrat sampling information and species counts, measurements, and tissue samples were collected concurrently with the photos.
DOI: 10.26008/1912/bco-dmo.751658.3
title: Site list for habitat complexity photos and related datasets sampled along the Northern Central California coast from 2017 and 2021

description: Site list for habitat complexity photos and related datasets sampled along the Northern Central California coast from 2017-2021.
DOI: 10.26008/1912/bco-dmo.750667.3
title: GPS waypoints for habitat complexity photos collected along the Northern Central California coast from 2017 to 2021

description: GPS waypoints for habitat complexity photos collected along the Northern Central California coast from 2017 to 2021.
DOI: 10.26008/1912/bco-dmo.939782.1
title: Oxygen consumption by lobster larvae at different temperatures using closed cell respirometry for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 to 2023 (Lobster Thermal Thresholds project)

description: We used the American lobster (Homarus americanus lsid:marinespecies.org:taxname:156134) in the Gulf of Maine as a model system to define thermal tolerance in larvae and establish mechanistic linkages between thermal tolerance of the individual larva and the patterns of settlement in the field.  We assessed and compared the thermal tolerances of larvae reared in the laboratory using conventional methods with larvae captured in the wild, and examined ontogenetic changes in thermal tolerance.  The upper and lower thermal thresholds larval stages I-IV and the first juvenile stage were defined in part by oxygen consumption and scope for activity (defined as the difference in oxygen consumption between larvae at rest and larvae swimming actively).  This data set includes the raw oxygen consumption data obtained through closed system microrespirometry and subsequently used to calculate scope for activity.  These data were collected between 2021-2023 at Bigelow Laboratory for Ocean Sciences, led by Eric Annis.
DOI: 10.26008/1912/bco-dmo.939825.1
title: Growth and mortality of lobster larvae at different temperatures for lobster larvae from Gulf of Maine near Boothbay Maine from 2021 and 2022 (Lobster Thermal Thresholds project)

description: We used the American lobster (Homarus americanus lsid:marinespecies.org:taxname:156134) in the Gulf of Maine as a model system to define thermal tolerance in larvae and establish mechanistic linkages between thermal tolerance of the individual larva and the patterns of settlement in the field.  We assessed and compared the thermal tolerances of larvae reared in the laboratory using conventional methods with larvae captured in the wild, and examined ontogenetic changes in thermal tolerance.  The upper and lower thermal thresholds larval stages I-IV and the first juvenile stage were defined in part by growth and mortality when subjected to chronic exposure to different treatment temperatures until they either molted to the next developmental stage or died.  This data set includes individuals dying, the size after molting and the amount of time elapsed between the start of the treatment and either molting or death.  These data were collected between 2021-2022 at Bigelow Laboratory for Ocean Sciences, led by Eric Annis.
DOI: 10.26008/1912/bco-dmo.751634.3
title: Quadrat sampling information along the Northern Central California coast from 2017 to 2021

description: Quadrat sampling information collected concurrently with habitat complexity photos along the Northern Central California coast from 2017 to 2021.
DOI: 10.26008/1912/bco-dmo.751650.3
title: Quadrat species counts and measurements along the Northern Central California coast from 2017 to 2021

description: Quadrat species counts and measurements collected concurrently with habitat complexity photos along the Northern Central California coast from 2017 to 2021.
DOI: 10.26008/1912/bco-dmo.952988.1
title: Potential microplastic counts of 6 common Caribbean spong species collected June 21, 2019 in Bocas del Toro, Panama

description: Microplastics (MP) are now considered ubiquitous across global aquatic environments. The ingestion of MP by fish and other marine vertebrates is well studied, but the ingestion of MP by marine invertebrates is not. Sponges (Phylum Porifera) are particularly understudied when it comes to MP ingestion, even though they are widely distributed across benthic habitats, can process large volumes of seawater, and can retain small particles within their water filtration systems. This study examines the presence of potential MP (PMP) in wild marine sponges and seawater collected in Bocas del Toro, Panamá. Subsurface seawater and tissue from six common Caribbean sponge species was collected in Saigon Bay, a heavily impacted, shallow-water coral reef. Seawater samples were filtered onto glass fiber filters to retain any PMP present and sponge tissue was digested with bleach, heated and filtered. Filters were examined using fluorescence microscopy to quantify PMP. An average of 107 ± 25 PMP L–1 was detected in seawater from Saigon Bay with particles ranging in size between 10 μm and ~3,000 μm. The number of PMP found in sponge tissue ranged between 6 ± 4 and 169 ± 71 PMP g–1 of dry tissue. Most particles found in sponge samples were very small (10–20 μm), but fibers greater than 5,000 μm were detected. Our results indicate that PMP exists within the tissues of the sponges we studied, but future studies should confirm the presence of MP in sponges using chemical analysis. Most importantly, the discrepancy between low levels of PMP in our sponge samples and high levels in the surrounding seawater highlights the potential for sponges to resist and/or egest MP. Finally, we provide a critical evaluation of our methods to improve their use in future MP work with benthic marine organisms.
DOI: 10.26008/1912/bco-dmo.955245.1
title: Literature review of sponge-microbe interactions on sixteen characteristics of 82 sponges.

description: This dataset compiles information from a literature review (Freeman et al., 2021) of seminal and recent papers focusing on sponge ecology and sponge-microbe interactions. Data (when available in the literature) on sixteen characteristics of 82 sponge species from diverse subclasses are included. These data include enumeration of overall microbial abundance (High and Low Microbial Abundance: HMA and LMA), chemical defense allocation patterns and overall palatability in feeding assays, nutritional quality (carbohydrate, protein, lipid, and energy content), physical characteristics (tensile strength, ash content, tissue density), photosymbiont abundance (chlorophyll a concentration) physiology and metabolism (pumping rate, NOx production), nutrition (% of carbon derived from dissolved organic matter, detritus, and living particulate organic matter), and microbial symbiont diversity (expressed as the inverse Simpson's index).
DOI: 10.26008/1912/bco-dmo.955389.1
title: Potential microplastic sizes of 6 common Caribbean spong species collected June 21, 2019 in Bocas del Toro, Panama

description: Microplastics (MP) are now considered ubiquitous across global aquatic environments. The ingestion of MP by fish and other marine vertebrates is well studied, but the ingestion of MP by marine invertebrates is not. Sponges (Phylum Porifera) are particularly understudied when it comes to MP ingestion, even though they are widely distributed across benthic habitats, can process large volumes of seawater, and can retain small particles within their water filtration systems. This study examines the presence of potential MP (PMP) in wild marine sponges and seawater collected in Bocas del Toro, Panamá. Subsurface seawater and tissue from six common Caribbean sponge species was collected in Saigon Bay, a heavily impacted, shallow-water coral reef. Seawater samples were filtered onto glass fiber filters to retain any PMP present and sponge tissue was digested with bleach, heated and filtered. Filters were examined using fluorescence microscopy to quantify PMP. An average of 107 ± 25 PMP L–1 was detected in seawater from Saigon Bay with particles ranging in size between 10 μm and ~3,000 μm. The number of PMP found in sponge tissue ranged between 6 ± 4 and 169 ± 71 PMP g–1 of dry tissue. Most particles found in sponge samples were very small (10–20 μm), but fibers greater than 5,000 μm were detected. Our results indicate that PMP exists within the tissues of the sponges we studied, but future studies should confirm the presence of MP in sponges using chemical analysis. Most importantly, the discrepancy between low levels of PMP in our sponge samples and high levels in the surrounding seawater highlights the potential for sponges to resist and/or egest MP. Finally, we provide a critical evaluation of our methods to improve their use in future MP work with benthic marine organisms.
DOI: 10.26008/1912/bco-dmo.954470.1
title: Bulk isotope data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021

description: Sponges represent one of the oldest extant animal phyla, and their associations with microbial symbionts have likely played a critical role in their success on oligotrophic coral reefs. For example, variation in the abundance, diversity, and community composition of microbiomes across host species may drive niche partitioning due to differential assimilation and recycling of nutrients across sponge species. Stable isotope analysis of bulk sponge tissue allowed us to test for evidence of partitioning of three major resource pools (dissolved organic matter: DOM, particulate organic matter: POM, and inorganic nutrients: NaHCO3 and NaNO3) among four emergent Caribbean sponge species. Results from bulk (holobiont tissue including both sponge and microbial cells) stable isotope analysis show niche partitioning, with strong interspecific variation in the relative exploitation of each resource pool. These results demonstrate that associations with microbial symbionts have facilitated niche partitioning by allowing host sponges to acquire and recycle diverse nutrient pools via unique mechanisms.
DOI: 10.26008/1912/bco-dmo.954735.1
title: Autotrophy, heterotrophy and niche partitioning in Caribbean sponges sampled June 9, 2019 on reef sites around Bocas del Toro Panama.

description: Photosymbionts expand the metabolic capabilities of host sponges, but their potential role in mediating niche partitioning on crowded and oligotrophic coral reefs is unknown. To address this question, we conducted two ex situ isotope tracer experiments with ten of the most ecologically dominant sponge species in the Caribbean. This research was carried out in Bocas del Toro, Panama. To target autotrophic and heterotrophic nutrient acquisition by microbial symbionts, we incubated sponges in seawater laced with the inorganic compounds NaH13CO3 and Na15NO3 under both light and dark conditions. We also measured host sponge heterotrophic feeding rates by incubating the same species with 13C- and 15N-labeled bacterial cells. Sponge cells isolated from sponge species hosting photosymbionts were significantly more enriched in 13C and 15N from inorganic sources, and 72 % of the variation in 13C and 15N enrichment across samples was explained by sponge species identity. Dark enrichment of 13C was minimal, but all species were enriched in 15N in the dark due to heterotrophic microbial nitrogen assimilation. Sponges rapidly consumed bacterial cells, but there was substantial variation in heterotrophic feeding rates among sponge species. When considering all three resource pools (symbiont autotrophy, symbiont heterotrophy, and sponge heterotrophy) and both elements, sponge species identity accounted for over 80 % of variation among specimens; in addition, we observed a clear separation of sponge species along a continuum of heterotrophic feeding on particulate organic matter to autotrophic metabolism via photosymbionts. These data demonstrate that the combined influence of sponge and photosymbiont metabolism enable coexisting sponge species to exploit unique resource pools on Caribbean reefs.
DOI: 10.26008/1912/bco-dmo.954346.1
title: Microbiome data from sponges collected in 2013 and 2014 from reefs in Honduras, Belize, Panama and the Florida Keys.

description: Marine sponges host diverse communities of microbial symbionts that expand the metabolic capabilities of their host, but the abundance and structure of these communities is highly variable across sponge species. Specificity in these interactions may fuel host niche partitioning on crowded coral reefs by allowing individual sponge species to exploit unique sources of carbon and nitrogen, but this hypothesis is yet to be tested. Given the presence of high sponge biomass and the coexistence of diverse sponge species, the Caribbean Sea provides a unique system in which to investigate this hypothesis. To test for ecological divergence among sympatric Caribbean sponges and investigate whether these trends are mediated by microbial symbionts, we measured stable isotope (δ13C and δ15N) ratios and characterized the microbial community structure of sponge species at sites within four regions spanning a 1700 km latitudinal gradient. Samples were collected in 2013 and 2014 from reefs in Honduras, Belize, Panama, and the Florida Keys. There was a low (median of 8.2 %) overlap in the isotopic niches of sympatric species; in addition, host identity accounted for over 75% of the dissimilarity in both δ13C and δ15N values and microbiome community structure among individual samples within a site. There was also a strong phylogenetic signal in both δ15N values and microbial community diversity across host phylogeny, as well as a correlation between microbial community structure and variation in δ13C and δ15N values across samples. Together, this evidence supports a hypothesis of strong evolutionary selection for ecological divergence across sponge lineages and suggests that this divergence is at least partially mediated by associations with microbial symbionts.
DOI: 10.26008/1912/bco-dmo.954439.1
title: NanoSIMS data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021

description: Sponges represent one of the oldest extant animal phyla, and their associations with microbial symbionts have likely played a critical role in their success on oligotrophic coral reefs. For example, variation in the abundance, diversity, and community composition of microbiomes across host species may drive niche partitioning due to differential assimilation and recycling of nutrients across sponge species. With Nanoscale secondary ion mass spectrometry (NanoSIMS), we evaluated the respective roles of host and microbial symbiont cells in the uptake and recycling of three resource pools (dissolved organic matter: DOM, particulate organic matter: POM, and inorganic nutrients: NaHCO3 and NaNO3) over 72 hr. NanoSIMS analysis revealed enrichment of individual host and symbiont cells over time and highlighted the differing roles of host and symbiont cells in the uptake and recycling of diverse sources of carbon and nitrogen within two of these species. For instance, dissolved organic matter uptake was mediated by microbial symbionts in one species and by host cells in another species. Together , these results demonstrate that associations with microbial symbionts have facilitated niche partitioning by allowing host sponges to acquire and recycle diverse nutrient pools via unique mechanisms. Field and lab work for this research was carried out in the Florida Keys during June/August of 2021.
DOI: 10.26008/1912/bco-dmo.954333.1
title: Isotopic data from sponges collected in 2013 and 2014 from reefs in Honduras, Belize, Panama and the Florida Keys.

description: Marine sponges host diverse communities of microbial symbionts that expand the metabolic capabilities of their host, but the abundance and structure of these communities is highly variable across sponge species. Specificity in these interactions may fuel host niche partitioning on crowded coral reefs by allowing individual sponge species to exploit unique sources of carbon and nitrogen, but this hypothesis is yet to be tested. Given the presence of high sponge biomass and the coexistence of diverse sponge species, the Caribbean Sea provides a unique system in which to investigate this hypothesis. To test for ecological divergence among sympatric Caribbean sponges and investigate whether these trends are mediated by microbial symbionts, we measured stable isotope (δ13C and δ15N) ratios and characterized the microbial community structure of sponge species at sites within four regions spanning a 1700 km latitudinal gradient. Samples were collected in 2013 and 2014 from reefs in Honduras, Belize, Panama, and the Florida Keys. There was a low (median of 8.2 %) overlap in the isotopic niches of sympatric species; in addition, host identity accounted for over 75% of the dissimilarity in both δ13C and δ15N values and microbiome community structure among individual samples within a site. There was also a strong phylogenetic signal in both δ15N values and microbial community diversity across host phylogeny, as well as a correlation between microbial community structure and variation in δ13C and δ15N values across samples. Together, this evidence supports a hypothesis of strong evolutionary selection for ecological divergence across sponge lineages and suggests that this divergence is at least partially mediated by associations with microbial symbionts.
DOI: 10.26008/1912/bco-dmo.957899.1
title: Diversity, distribution, and temporal stability of coral 'zooxanthellae' on a Pacific reef evaluated with samples collected in Palau from 2013 to 2022

description: This data set provides information about the long-term composition of symbiont populations within individual coral colonies. Diverse coral taxa (Scleractinia) from a West Indo-Pacific fore reef (Palau) were tagged and sampled at various intervals--ranging from six months to several years--over nine years' time. Symbiont identity was examined using multiple genetic markers that resolved symbiont diversity to species and individual genotypes (i.e. clonal strains). Consistent with previous colony monitoring studies, symbiont populations in a majority of colonies were dominated by one species and one strain (based on multi-locus genotyping) over multiple years. Thus, the distribution of symbiont diversity at the genus, species and clone level, comprising specific and stable partner combinations, scale predictably to reef habitat, host taxon, and individual colony.
DOI: 10.26008/1912/bco-dmo.959247.1
title: Pore water data from sediment cores collected from R/V Oceanus cruise OC1906A and R/V Sikuliaq cruise SKQ202016S off the coast of California in 2019 and 2020

description: Sediment pore waters were determined in cores collected at several stations off the coast of California: at a site in Santa Barbara Basin ('SBB'; latitude: 34.223, Longitude: -119.986; water depth, 590 m); Catalina Basin ('Cat'; latitude: 33.301, longitude: -118.6; water depth, 1310 m); and two sites off the coast of central California south of Monterey Bay; site 'K' (latitude 35.375, longitude -121.501, water depth, 1000 m) and site 'D' (latitude 36.113, longitude: -122.186, water depth, 1440 m). The Cat cores were collected in June 2019, while the site K and D cores were collected in December 2020. Pore waters were collected either using rhizon samplers or by centrifugation of sectioned sediments. Pore water samples were analyzed for alkalinity on-board the ship, while other analyses were returned to the shore-based lab at Old Dominon University (ODU) where they were analyzed. These samples were collected as part of a larger project examining deaminated peptides and organic matter cycling in the sediments at these sites.
DOI: 10.26008/1912/bco-dmo.958726.1
title: Larval fish counts and larval tuna counts with corresponding plankton net type and geographic coordinates from R/V Roger Revelle cruise RR2201 (BLOOFINZ-IO, January-March 2022) in the Argo Basin region off NW Australia

description: This dataset contains larval fish counts and larval tuna counts with corresponding plankton net type and geographic coordinates from cruise RR2201 of R/V Roger Revelle (BLOOFINZ-IO, January-March 2022) in the Argo Basin region off northwestern Australia.
DOI: 10.26008/1912/bco-dmo.959217.1
title: Sediment porosity collected from R/V Oceanus cruise OC1906A and R/V Sikuliaq cruise SKQ202016S off the coast of California in 2019 and 2020

description: Sediment porosity was determined in cores collected at several stations off the coast of California: at a site in Catalina Basin ('Cat'; latitude: 33.301, longitude: -118.6; water depth, 1310 m); sites K and D off the coast of central California south of Monterey Bay ('K'; latitude: 35.375, longitude: -121.501; water depth, 1000 m; 'D'; latitude: 36.113, longitude: -122.186; water depth, 1440 m). The Cat cores were collected in June 2019, while the site K and D cores were collected in December 2020. Sediment cores were sectioned on-board the ship, and wet sediment was placed in pre-weighed scintillation vials. The vials were frozen and returned to the shore-based lab at Old Dominon University (ODU) where the samples were dried to constant weight and wet-to-dry sediment ratios were converted to porosity values. These samples were collected as part of a larger project examining deaminated peptides and organic matter cycling in the sediments at these sites.
DOI: 10.26008/1912/bco-dmo.948204.1
title: Behavioral data of Nucella lamellosa in the presence of predators within laboratory mesocosms at Bodega Marine Lab in 2018

description: To examine the intensity of anti-predatory behavior of both Tegula funebralis and Nucella lamellosa, we measured the proportion of time individual snails spend above the waterline in the presence and absence of their predator species, Pisaster ochraceus and Cancer productus. Data from this experiment are used to calculate estimated predation rates for snails that do not behaviorally respond to their predators, which are used to determine the impact anti-predatory responses have on the strength of trophic cascades. The experiment was conducted within laboratory mesocosms at Bodega Marine Lab in 2018 and 2019.

This dataset reports results from mesocosms in this experiment with Nucella lamellosa in the presence of predator cues. See &quot;Related Datasets&quot; section for results from other mesocosms and experiments to be published in Ng & Gaylord (2025, in-prep).