cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.9qvfmb
title: Occurrence Download

description: A dataset containing 181103 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aves",
 "Year is greater than or equal to 1900",
 "Year is less than or equal to 1990",
 "Geometry MULTIPOLYGON (((-82 9, -82 10, -83 10, -84 10, -84 9, -83 9, -82 9)), ((-80 7, -80 8, -81 8, -81 7, -80 7)), ((-81 8, -81 9, -82 9, -82 8, -81 8)), ((-85 10, -85 11, -86 11, -86 10, -85 10)), ((-96 19, -96 20, -97 20, -97 19, -96 19)), ((-101 18, -101 17, -100 17, -99 17, -99 16, -98 16, -97 16, -96 16, -95 16, -95 17, -96 17, -97 17, -97 19, -98 19, -98 20, -97 20, -97 21, -98 21, -98 24, -99 24, -99 25, -100 25, -101 25, -101 24, -100 24, -100 20, -101 20, -101 21, -102 21, -102 22, -103 22, -103 23, -104 23, -104 24, -105 24, -105 25, -106 25, -106 27, -107 27, -107 28, -108 28, -108 29, -109 29, -109 26, -108 26, -108 25, -107 25, -107 23, -106 23, -106 22, -105 22, -105 19, -104 19, -104 18, -103 18, -102 18, -101 18)), ((-88 15, -89 15, -90 15, -90 14, -89 14, -89 13, -88 13, -87 13, -87 12, -86 12, -85 12, -85 13, -86 13, -86 15, -87 15, -88 15)), ((-83 14, -83 15, -84 15, -84 14, -83 14)), ((-91 15, -91 16, -92 16, -92 15, -91 15)), ((-92 18, -92 19, -93 19, -93 18, -92 18)), ((-89 17, -88 17, -88 19, -87 19, -87 21, -88 21, -88 22, -89 22, -89 20, -90 20, -91 20, -91 18, -91 17, -90 17, -89 17)))",
 {
 "not" : {
 "BasisOfRecord" : [
 "is Specimen"
 ]
 }
 }
 ]
}

 The dataset includes 181103 records from 27 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003160-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.21227/00ym-r151
title: "image data"

description: "This is  an image dataset."
DOI: 10.15468/dl.j7gchp
title: Occurrence Download

description: A dataset containing 51370 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Magnoliopsida",
 "Country is Svalbard and Jan Mayen",
 "DecimalLatitude is not null",
 "DecimalLongitude is not null"
 ]
}

 The dataset includes 51370 records from 77 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003203-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8yqdxb
title: Occurrence Download

description: A dataset containing 34705 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Cynosurus echinatus"
 ]
}

 The dataset includes 34705 records from 585 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003234-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8hd3dk
title: Occurrence Download

description: A dataset containing 420202 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Observation, Machine Observation, Human Observation)",
 "Country is Indonesia",
 "DatasetKey is one of (iNaturalist Research-grade Observations, Observation.org, Nature data from around the World, Biodiverskripsi: Biodiversity Theses Database)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "PublishingOrg is one of (28eb1a3f-1c15-4a95-931a-4af90ecb574d, c8d737e0-2ff8-42e8-b8fc-6b805d26fc5f)",
 "Year 2010-2025"
 ]
}

 The dataset includes 420202 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003206-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.yvatt8
title: Occurrence Download

description: A dataset containing 5792519 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "OccurrenceStatus is Present",
 "Year 2000-2025"
 ]
}

 The dataset includes 5792519 records from 3761 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003188-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.y5k3vq
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Medicago arabica",
 "Country is Latvia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0050113-250811113504898/datasets/export for details.
DOI: 10.18419/darus-4765
title: arm26 Version for: Closed-Loop Coupling of Both Physiological Spindle Model and Spinal Pathways for Sensorimotor Control of Human Center-Out Reaching

description: &lt;p&gt;Version of the arm26 model (&lt;a href="https://doi.org/10.18419/DARUS-2871"&gt;https://doi.org/10.18419/DARUS-2871&lt;/a&gt;) used in the study 'Closed-loop coupling of both physiological spindle model and spinal pathways for sensorimotor control of human center-out reaching', including additional scripts to run with python, muscle spindle module and NEST simulator. &lt;br&gt;
The file contains an archive including all relevant data to run the simulation in the simulator demoa. This needs to be installed separately and is available as open source too (get-demoa.com).&lt;/p&gt;

If you use this model, please cite the related publication together with this dataset.
description: More information can be found in the README.md.
DOI: 10.15468/dl.vr46vt
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Medicago arabica",
 "Country is Poland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0050114-250811113504898/datasets/export for details.
DOI: 10.5281/zenodo.16953155
title: Artifact for TACAS'25 paper: Performance Heuristics for GR(1) Realizability Checking and Related Analyses

description: Artifact for TACAS'25 paper: Performance Heuristics for GR(1) Realizability Checking and Related Analyses
DOI: 10.5281/zenodo.14616533
title: Artifact for TACAS'25 paper: Performance Heuristics for GR(1) Realizability Checking and Related Analyses

description: Artifact for TACAS'25 paper: Performance Heuristics for GR(1) Realizability Checking and Related Analyses
DOI: 10.5281/zenodo.16964585
title: Rate and GITT Test Data for NaCR32140 Na-ion Battery Cells Between 15 degC and 45 degC
DOI: 10.5281/zenodo.16964586
title: Rate and GITT Test Data for NaCR32140 Na-ion Battery Cells Between 15 degC and 45 degC
DOI: 10.22002/sde09-4e736
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-08-15</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2017-08-15-41_slicer18758.jpg: cytoplasmic vesicle dga2017-08-15-41_slicer18759.jpg: cytoplasmic filaments
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-41/rawdata/</p>Cb_20170816_Cb1pH4d50040.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-41/rawdata/Cb_20170816_Cb1pH4d50040.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_20170816_Cb1pH4d50040_part61_1.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-08-15-41/3dimage_88023/Cb_20170816_Cb1pH4d50040_part61_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pfv9d-k0m07
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-09-25</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.T</p>
  dga2017-09-25-5_slicer18909.jpg: multilayered structures beneath the inner membrane dga2017-09-25-5_slicer18910.jpg: extracellular vesicular structure
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-5/rawdata/</p>Cb_vero_201709250005.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-5/rawdata/Cb_vero_201709250005.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_201709250005_part61_2.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-5/3dimage_88390/Cb_vero_201709250005_part61_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ptxy3-k4n50
title: Agro_AD1231

description: AD1231: pAD1361/A136; Carbenicillin 50;  virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.
description: <strong>Tilt Series Date:</strong> 2015-08-03</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 155.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow culture in AB medium (pH 7) at 30C. For induction, use 375 µl of a decent ON culture to inoculate 5 ml AB mes pH 5.8 medium containing 100 µM Acetosyringone and antibiotic (Cb 50). Grow at 20C for 18 hr. Concentrate cells 5 times by centrifuging. Freeze cells.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK III, MK IV was not working) settings: 100% humidity, 4µl sample, 5% BSA treated gold. Blot offset -5, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  dga2015-08-03-27_slicer14081.jpg: ?? dga2015-08-03-27_slicer14082.jpg: ??
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-27/3dimage_60029/</p>20150803_1231_10024.rec,  Reconstruction (Pixel Size 1.92 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-27/3dimage_60029/20150803_1231_10024.rec"
        > <i class="download icon"></i> Download </a></p>

        20150803_1231_10024.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-27/rawdata/20150803_1231_10024.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/enbdz-jxw23
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-09-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~15 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5 µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-90/3dimage_61355/</p>20150909_AK_pMT15_30039_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-90/3dimage_61355/20150909_AK_pMT15_30039_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20150909_AK_pMT15_30039.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-90/rawdata/20150909_AK_pMT15_30039.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x5jjm-8h242
title: FtsZ structure at dividing site

description: ingle tilt. Constant angular increment. FtsZ. Complete ring. Caulobacter.
description: <strong>Tilt Series Date:</strong> 2015-03-10</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo</p>
<strong>Purification / Growth Conditions / Treatment:</strong> C. crescentus was from Yi-Wei's cryo-stock and was grown in PYE medium overnight at 30°c 250 rpm. In the next days' morning, 1/20 subculture into the M2G medium. When OD600 reaches 0.48, 1ml medium was spun down and the resuspension was 100 ul for cryo-grid preparation.</p>
<strong>Sample Preparation:</strong> Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 120/A2. defocus: -10um. magnification: 22500.</p>
  qya2015-03-10-11_slicer12851.jpg: Invagination on both sides.
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-03-10-11/rawdata/</p>Qing15_10011.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-03-10-11/rawdata/Qing15_10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        Qing15_10011_part121_5.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-03-10-11/3dimage_53042/Qing15_10011_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.wan4ku
title: Occurrence Download

description: A dataset containing 68 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Venezuela (Bolivarian Republic of)",
 "TaxonKey is Rhinella sternosignata"
 ]
}

 The dataset includes 68 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006180-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/er019-eg474
title: C.crescentus, no FtsZ ring

description: Caulobacter crescentus NA1000, single cell, free-living, none-dividing. no FtsZ ring
description: <strong>Tilt Series Date:</strong> 2015-02-17</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> C. crescentus was from Yi-Wei's cryo-stock and was grown in PYE medium overnight at 30°c 250 rpm. In the next days' morning, 1/20 subculture into the M2G medium. When OD600 reaches 0.48, 1ml medium was spun down and the resuspension was 100 ul for cryo-grid preparation.</p>
<strong>Sample Preparation:</strong> Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 120/A2. defocus: -10um. magnification: 22500</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-02-17-51/rawdata/</p>ZMatlas20015.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-02-17-51/rawdata/ZMatlas20015.mrc"
        > <i class="download icon"></i> Download </a></p>

        ZMatlas20015_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-02-17-51/3dimage_52115/ZMatlas20015_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/h5btz-00y85
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-12-19</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, place the tube on bench for ~20 min, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~20 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-19/3dimage_71589/</p>20151219_YWC206_10019_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-19/3dimage_71589/20151219_YWC206_10019_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20151219_YWC206_10019.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-19/rawdata/20151219_YWC206_10019.mrc"
        > <i class="download icon"></i> Download </a></p>