DOI: 10.5061/dryad.ht76hdrn2
title: Data from: Divergence in the internal genital morphology of females is correlated with divergence in male intromittent structures among populations of a millipede

description: Our understanding of genital evolution comes largely from studies of male
 genitalia. Females have received far less attention because of the
 difficulties inherent in quantifying the shapes of their internal genital
 structures. Here we combine advances in micro-computed tomography with a
 new landmark free method of quantifying three-dimensional trait shape, to
 document patterns of divergence in female genital shape, and the
 correlated divergence of male genitalia among populations of the millipede
 Antichiropus variabilis. We used single nucleotide polymorphisms to
 estimate levels of neutral genetic divergence among seven populations of
 millipede. Genetic divergence was high and correlated with geographic
 distance. Comparing phenotypic divergence in genital shape to neutral
 genetic divergence, we found that genital shape for both females and males
 has diverged more than would be expected from random drift, consistent
 with a pattern of directional selection. While there was significant
 covariation between female and male genital shape across populations, the
 magnitude of divergence in genital shape between the sexes was not
 correlated. Our results demonstrate the utility of using three-dimensional
 scanning technologies to examine female genital traits and add to a small
 but growing number of studies showing that like male genitalia, female
 genitalia can be under strong directional selection.
description: Millipedes collected from the field, stained and scanned using
 micro-CT. Surface meshes used in GPSA to extract 3D shape data for
 downstream statistical analyses.  DNA sequences and neutral SNPs
 identified for analysis of population genemtic parameters.
description: These files provide the raw data necessary to repeat the analyses
 presented in Sloan & Simmons, Divergence in the internal genital
 morphology of females correlated with divergence in male intromittent
 structures among populations of a millipede. Evolution, 2023.   IDs
 Individuals are labelled by their geographic origin: Gingin, Avon Valley,
 Nalya Park, Kings Park (both KPJ and Thomas Road), Ellis Brook, Beeliar
 and Serpentine (e.g. Avon1\_m is male 1 collected from Avon valley.  
 Morphology PLY (polygon file format) files are the surface mesh files
 extracted from Avizo v9.2.0. Each .ply file contains the 3D scan data (x,
 y, z coordinates) that describe the surface mesh for each male gonopod and
 each female receptaculum. These files can be entered into the Generalized
 Procrustes Surface Analysis (GPSA) software available in Pomidor et al.
 (2016) to reproduce the shape variables provided in the 
 gpsa\_ordination\_projection files. The .ply files can also be opened in
 any graphics package to visualize the surface mesh for each individual.
 The gpsa\_ordination files contain the data used in downstream analyses.
 The columns, Axis1 – Axis*<sub>i</sub>* are the scores of each
 individual on the first to ith major axis from the principal coordinates
 analysis.  Collum area is also provided for each individual.   Genetics
 Two files are provided;  the raw SNP data from DArT, and the set of
 neutral SNP loci following outlier detection. Cells in this file
 containing NA represent SNPs that could not be scored.   Pomidor B.J.,
 Makedonska J., Slice D.E. 2016 A landmark-free method for
 three-dimensional shape analysis. *PLOS ONE* **11**(3), e0150368.
 (doi:10.1371/journal.pone.0150368).