DOI: 10.5061/dryad.cnp5hqcbn
title: Microsatellite data of bank voles and <em>Ixodes ricinus</em> plus TBEV nucleotide sequences

description: Tick-borne encephalitis (TBE) virus is considered the medically most
 important arthropod-borne virus in Europe. Although TBE is endemic
 throughout central Europe, ticks and rodents determine its maintenance in
 small, difficult-to-asses, natural foci. We investigated the interrelation
 between the genetics of the main TBE virus (TBEV) vector tick (Ixodes
 ricinus), the most important reservoir host (Myodes glareolus, syn.
 Clethrionomys glareolus), and TBEV. Rodents and ticks were sampled on 15
 sites within an exploratory study area, which has been screened regularly
 for TBEV occurrence in ticks for more than 10 years. On all 15 sites,
 ticks and bank voles were sampled, screened for TBEV presence via serology
 and RT-PCR, and genetically examined. Moreover, TBEV isolates derived from
 these analyses were sequenced. In long-term TBEV foci bank vole
 populations show extraordinary genetic constitutions, leading to a
 particular population structure, whereas ticks revealed a panmictic
 genetic structure over all sampling sites. Landscape genetics and habitat
 connectivity modeling (analysis of Isolation by resistance) showed no
 landscape-related barriers explaining the genetic structure of the bank
 vole populations. The results suggest that bank voles do not simply serve
 as TBEV reservoirs, but their genetic composition has a significant
 influence on establishing and maintaining long-term natural TBEV foci,
 whereas the genetic structure of TBEV´s main vector Ixodes ricinus does
 not play an important role in the sustainability of long-term TBEV foci.
description: Bank voles were sampled in 2019 using Sherman Traps.
 Ticks were sampled in 2019 using the flaggin method.
 TBEV isolates were dervied from positive samples.
description: # Microsatellite data Myodes glareolus and Ixodes ricinus
 [https://doi.org/10.5061/dryad.cnp5hqcbn](https://doi.org/10.5061/dryad.cnp5hqcbn) The dataset consists of Microsatellite data of *Myodes glareolus* and *Ixodes ricinus* and Sanger sequenced Sequences of TBEV isolates for the article "Interrelation of the spatial and genetic structure of tick-borne encephalitis virus, its reservoir host (Myodes glareolus), and its vector (Ixodes ricinus) in a natural focus area". ## Description of the data and file structure The dataset "`Microsatellite_data_Mg_Ir.xlsx`" has the microsatellite data of *Myodes glareolus* on one sheet and of *Ixodes ricinus* on the other sheet. Empty cells represent no data, since this locus failed the amplification, or gel electrophoresis or the signal was unclear. Some column headers are blank, due to downstream software requirements (Microsatellite Toolkit).  TBEV isolates where aligned and trimmed (`FSME_Seqs_all_aln_trim_6_W_Paper.fas`) and can be used for further analysis. Metadata (Smaple ID (ID), coordinates of sampling location (X,Y) and TBEV status of samples/individuals (TBEV Status) can be found in the metadata file (`Metadata_Mg_Ir_TBEV.xlsx`). The third sheet "TBEV" gives the sampling location of the bank voles or ticks, of whom TBEV isolates were isolated.