cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.3377844
title: Chemical ionization quadrupole mass spectrometer with an electrical discharge ion source for atmospheric trace gas measurement (dataset)

description: Data from field studies provided in Fig. 6 (CYPHEX), 7 (NOTOMO) and 8 (IBAIRN) of Eger, P. G., Helleis, F., Schuster, G., Phillips, G. J., Lelieveld, J., and Crowley, J. N.: Chemical ionization quadrupole mass spectrometer with an electrical discharge ion source for atmospheric trace gas measurement, Atmos. Meas. Tech., 12, 1935–1954, https://doi.org/10.5194/amt-12-1935-2019, 2019.
DOI: 10.5281/zenodo.3377845
title: Chemical ionization quadrupole mass spectrometer with an electrical discharge ion source for atmospheric trace gas measurement (dataset)

description: Data from field studies provided in Fig. 6 (CYPHEX), 7 (NOTOMO) and 8 (IBAIRN) of Eger, P. G., Helleis, F., Schuster, G., Phillips, G. J., Lelieveld, J., and Crowley, J. N.: Chemical ionization quadrupole mass spectrometer with an electrical discharge ion source for atmospheric trace gas measurement, Atmos. Meas. Tech., 12, 1935–1954, https://doi.org/10.5194/amt-12-1935-2019, 2019.
DOI: 10.14457/cu.the.2010.753
title: Alpha-glucosidase inhibitory activity of protein from seeds of djenkol bean Archidendron jiringa Nielsen

description: The inhibitors of α-glucosidase enzyme from natural resource which is responding to digest carbohydrates to monosaccharides. It has been used for the treatment of diabetes mellitus type II or non insulin dependent diabetes mellitus. Legumes were reported as a major source of proteins and polypeptides with important biological activities. One of the biological activities in legume is α-glucosidase inhibitory activity. This study was attempted to isolate α-glucosidase inhibitors from two leguminous plants: Archidendron jiringa Nielsen. (Djenkol bean) and Parkia speciosa Hassk. (Stink bean). The potential α-glucosidase inhibitor was found in the protein extraction from seeds of A. jiringa when used Tris-HCl buffer for extraction then precipitated with 90% ammonium sulfate, and purify with affinity chromatography using ConA Sepharose as stationary phase. The bound protein gave inhibitory activity against α-glucosidase using p-nitrophenyl α-D-glucopyranoside (PNPG) with the IC₅₀ value of 0.031 mg protein. The molecular mass of the purified protein was 35.7 kDa, as estimated by SDS-PAGE. The protein was thermostable up to 80 °C for 70 min and showed an optimum activity within the pH range of 8.0-10.0. It has a high activity with divalent cations such as Cu²⁺, Zn²⁺ and Fe²⁺. The internal amino acid sequence of the protein showed that is similar to the sequences of lectin from Dioclea guainensis. Moreover, this α-glucosidase inhibitor has specific interaction with α-glucosidase enzyme which was confirmed by Surface Plasmon Resonance (SPR) technique.
DOI: 10.14457/cu.the.2010.754
title: Purification and characterization of laccase from white rot fungus WR77 and its applcation in decoloriztion of syntherization of synthetic dyes

description: It is well known that laccase produced by white rot fungi were able to decolorize synthetic dyes. Therefore in this research white rot fungi were screened for high laccase activity with new characteristic. Selective white rot fungi produced high laccase activity by growing on medium supplemented with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS). White rot fungus strain WR77 showed highest laccase activity among other isolates. WR77 was tested for the effect of carbon source and nitrogen source on highest laccase production. One percent (w/v) rice chaff, 0.5 g/L di-ammonium tartrate and 0.01 g/L peptone were supplemented in the medium as carbon and nitrogen sources showed highest laccase activity. Purification WR77 laccase by ammonium sulphate precipitation, Q-sapharose anion-exchange chromatography, and Sephadex G-75 gel filtration chromatography showed enzyme activity as 617.12 U/mg protein. The enzyme has molecular mass of approximately 75.2 kDa as determined by SDS-PAGE. Purified WR77 laccase remained active at pH range 4.0 to 11.0 at 40°C for up to 120 min of incubation time. The Km value of enzyme for ABTS as substrate was 447.93 mM and its corresponding Vmax value of 104.17 µmol/min/mg protein. 5.0 mM Hg²⁺ and 1.0 mM Fe²⁺ inhibited WR77 laccase activity whereas 10 mM Cu²⁺ ions stimulated WR77 laccase activity by 9.7%. WR77 could decolorized 98% Ambifix Blue H3R was in 8 days, 24% Ambifix Yellow H3R in 10 days and 50% Ambifix Red HE3B in 18 days. Crude laccase with concentration 5 U/ml could also decolorize 65% Ambifix Blue H3R within 15min and 80% Malachite Green within 24hr.
DOI: 10.14457/cu.the.2010.755
title: Expression and characterization of recombinant endoglucanase from Trichoderma reesei for the production of cellulosic ethanol

description: Bioethanol, unlike petroleum, is a form of renewable energy that can be produced from agricultural feedstocks. It can be used as a transport fuel, mainly as a biofuel additive for gasoline. The production of ethanol from lignocellulose has the advantage of abundant and diverse raw material compared to other sources like corn and cane sugars. However, it requires one more additional step; saccharification which the lignocellulosic biomass is hydrolyzed to glucose before converted to ethanol by fermentation. Recently, the hydrolysis of cellulose using biocatalysts, cellulase has been interestingly increased. Nevertheless, natural microbial cellulase cannot be sufficiently produced for the industrial application. Therefore, this research attempted to clone and express the genes of endoglucanase; eg1 and eg2 from Trichoderma reesei, one of the most effective producers of cellulase. From the results, the eg1 gene was composed of 713 bps, encoding 234 amino acids, molecular weight approximately 25 kD while eg2 gene was composed of 1257 bps, encoding 418 amino acids, molecular weight approximately 44 kD. The recombinant plasmid, pPICαA-eg1 and pPICαA-eg2, were transformed into Pichia pastoris X33 by electroporation. The transformants expressing the endoglucanase1 (EG1) and endoglucanase2 (EG2) were selected for optimizing expression conditions. The EG1 and EG2 were highly expressed by 4% and 5% methanol induction for 5 days, respectively. EG1 showed the highest activity at pH 5.0 and 60°C and it was stable at pH range of 4.0–5.5 and temperature below 75°C. EG2 showed the highest activity at pH 5.0 and 70°C and it was stable at pH range of 3.5–5.5 and temperature below 70°C. The steady-state kinetic study indicated that the EG2 was six folds greater catalytic efficiency toward carboxymethyl cellulose substrate than EG1. However, both of them could hydrolyze varieties of cellulosic substrate including lignocellulosic materials. From this study, it could be concluded that endoglucanase produced from recombinant Pichia pastoris can be applied for saccharification process in bioethanol production.
DOI: 10.14457/cu.the.2010.756
title: Preparation of rigid polyurethane foam catalyzed by Cu-amine and Mn-amine complexes

description: The complexes of metal-amine [M(en)₂ and M(trien)] and metal-amine-salicylate [M(en)₂Sal₂ and M(trien)Sal₂], where M = Cu and Mn, were synthesized. The metal complexes were used as the catalysts in the preparation of rigid polyurethane foams. The reaction times, physical and mechanical properties of the prepared foams were investigated and compared to the foam prepared by commercial catalyst (N,N-dimethylcyclohexylamine, DMCHA). FTIR, UV-vis spectroscopy, elemental analysis and X-ray diffraction were used to characterize the catalysts. ATR-IR technique was used to determine isocyanate (NCO) conversion and polyisocyanurate:polyurethane (PIR:PUR) ratio in the polyurethane foam. The foaming reaction is an exothermic reaction. The maximum polymerization temperature is in the range of 100-136 °C. During foam preparation, the cream time, gel time, tack free time and rise time were investigated. The results showed that the apparent density of prepared foams was in the range 39.0-50.0 kg/m³. Cu(en)₂ and Cu(trien) catalysts showed better catalytic activity than DMCHA catalyst. Furthermore, the compressive strengths of rigid polyurethane foams catalyzed by Cu(en)₂, Cu(trien) and Cu(trien)Sal₂ were better than that of DMCHA. At NCO index of 100, their compressive strength values were 257.0, 237.7 and 255.2 kPa, respectively. While at NCO index of 150, their compressive strength values were 370.6, 338.5 and 357.3 kPa, respectively.
DOI: 10.14457/cu.the.2010.757
title: Effects of alkyl chain length on thermochromism of polydiacetylene containing two amide groups

description: Five symmetrical and six unsymmetrical diyndiamide monomers were prepared. Their thermochromic properties were investigated in order to probe the effects of methylene units within the linker (x) and hydrophobic tail (y) on thermochromic reversibility. All synthesized monomers were dispersed well in Milli-Q water by ultrasonication. The dispersion could be converted to blue sols of polydiacetylenes (PDA) via UV irradiation. In case of PDA derived from symmetrical diyndiamides, high color transition temperature (CTT) (70-80 °C) and high degree of color reversibility (%DR = 88-93) were observed with PDAs containing even methylene linkers while the PDAs with odd methylene linkers showed low CTT (35-45 °C) and low degree of color reversibility (%DR = 20-65). For the PDAs derived from unsymmetrical diyndiamide, %DR of the PDAs increased upon raising the number of methylene units within the hydrophobic tail (y). The results indicate that the number of methylene units play an important role in thermochromic reversibility providing the strategy in molecular design for thermal sensor development.
DOI: 10.14457/cu.the.2010.758
title: Transglycosylation reaction catalyzed by cyclodextrin glycosyltransferase and amylomaltase for the synthesis of oligosaccharided with anticariogenetic property

description: This work aims to synthesize maltosylsucrose using cyclodextrin glycosyltransferase (CGTase) from Paenibacillus sp. RB01 and recombinant amylomaltase from Corynebacterium glutamicum through the transglycosylation reaction with various glucosyl donors and sucrose acceptor. This is the first report on using amylomaltase for the synthesis of maltosylsucrose. In the analysis of product by TLC and HPLC on a Rezex RSO-Oligosaccharide column, the suitable glucosyl donor for CGTase and amylomaltase were soluble and raw tapioca starch, respectively. The optimal condition for the synthesis of maltosylsucrose from CGTase was 2.5% (w/v) sucrose, 20% (w/v) tapioca starch (soluble), 400 U/mL (dextrinizing unit) of CGTase at 40°C for 18 hours and that for amylomaltase was 2.0% (w/v) sucrose, 2.5% (w/v) tapioca starch (raw), 9 U/ml (disproportionation unit) of amylomaltase at 30°C for 48 hours. CGTase was more efficient than amylomaltase for this synthetic reaction in which the total percent yields of the transfer products from CGTase and amylomaltase were about 98% and 81%, respectively. The products were isolated by Biogel P-2 gel column, each peak was analyzed by HPLC and mass spectrometry. The major transfer products of both enzymes were G₂F, G₃F, G₄F and G₅F with different product ratio. The ratios were 1.0:1.2:1.2:1.3 and 1.0:0.9:0.8:0.7 for CGTase and amylomaltase, respectively. Higher ratio of larger products (G₇F, G₈F and &gt; G₈F) could be obtained from both enzymes at low amounts of sucrose and enzyme. Low cariogenic property of maltosylsucrose products was confirmed by comparing the synthesis of water insoluble glucan, acid fermentation, plaque formation and cell aggregation of Streptococcus mutans to those exerted by sucrose. By adding sucrose to maltosylsucrose products in the ratios of 1:1, 1:2 and 1:4, inhibitory effects on glucosyltransferase activity of S. mutans by 7, 33 and 50% were observed. These results suggested that maltosylsucrose products were low cariogenic oligosaccharides.
DOI: 10.14457/cu.the.2010.759
title: Preparation and characterization of rare earth ions-doped TiO₂ photocatalyst film by RF-magnetron sputtering

description: This research studied on the preparation and the effect of Yb₂O₃ and Er₂O₃ doped TiO₂ on the surface morphology, optical and photocatalyst properties under UV and fluorescent irradiation of thin films via RF-magnetron sputtering method. The preparation conditions were fixed for applied power, distance between target-substrate and substrate heating temperature at 150 W, 5.0 cm and 500 °C, respectively. All deposited films were controlled the thickness at about 1.0 μm by varying deposition time. As the results, all of prepared films showed only pure anatase phase. The sputtering pressure and sputtering gas had significantly affected on the microstructure of TiO₂ film. The films prepared under high pressure (3 Pa) and pure Ar gas displayed porous structure and high surface roughness, resulted in high photocatalytic activity. While the TiO₂ films prepared under gas mixture Ar/O₂ (8/2) showed porous structure with lower surface roughness resulted in lower photocatalytic activity than that of film prepared under pure Ar gas. In case of TiO₂ films doping with Yb₂O₃ and Er₂O₃, the increasing in surface roughness and the decreasing in transmittance of the films prepared under gas mixture of Ar/O₂ (8/2) when concentration of Yb₂O₃ and Er₂O₃ target increased from 0.5 to 1.0 mol% was observed. Moreover, the absorption thresholds slightly shifted to longer wavelength which was attributed to the decreases in band gap energy. And higher photocatalytic activity under UV and Fluorescent irradiation was obtained. The similar result of absorption and band gap energy of the films prepared under pure Ar was also observed. But the difference in surface roughness was not significantly affected as the concentration of dopant increased. In addition, the doped films prepared under gas mixture had better photocatalytic activity than that of films prepared under pure Ar gas.
DOI: 10.14457/cu.the.2010.760
title: Improving the oxygen permeability of barium or lanthanum-based perovskite membranes by surface-coating with catalysts

description: The effect of SrCoFeOx (SCF), La₀₁Sr₀.₉Co₀.₉Fe₀₁O₃ (LSCF) and SrCo₀.₄Fe₀.₅Ni₀₁O₃ (SCFN) thin layers, prepared by modified citrate method, coating on oxygen permeation flux through dense Ba₀.₅Sr₀.₅Co₀.₈Fe₀.₂O₃ (BSCF) membranes and BaCo₀.₇Fe₀.₂Nb₀.₁O₃ (BCFN) membranes, synthesized via solid state method, was investigated. Unfortunately, the relative density of prepared BCFN membrane (79.28%) was quite lower than the required 90% for gas permeation membrane; therefore, only BSCF membrane was further studied. The catalyst powders were coated on one or both surfaces of dense BSCF membranes. The oxygen permeation flux of membranes was measured in the temperature range of 700-1000°C. The results show that the oxygen permeation fluxes of most coated membranes were higher than the flux of uncoated BSCF membrane. Comparing the effect of single-coating on air side, coating of SCF showed higher flux than coating with LSCF and SCFN. On the other hand, comparing the effect on He side, coating with SCFN gave higher flux than LSCF and SCF. Furthermore, double coating with SCF on air side and with SCFN on He side increased the flux of a single-coating and has good oxygen permeation stability at 850°C for at least 100h. The chemical compatibility of membrane and catalysts was tested by firing at high temperature for 10 h. BSCF membrane has good compatibility with SCF and SCFN at 850°C and with LSCF at 900°C.
DOI: 10.14457/cu.the.2010.761
title: Sample preparation by matrix solid-phase dispersion for determination of organochlorine and pyrethroid residues in garlic

description: Matrix solid-phase dispersion (MSPD) is a simple and fast sample preparation technique that employs very small amount of organic solvent. Sample is dispersed uniformly in the solid support where the matrix and interferences are trapped within the sorbent and the target analytes can be eluted selectively with suitable organic solvent. This research developed a MSPD procedure for the extraction and cleanup of twenty six organochlorine and pyrethroid residues (OCPYs) in garlic for GC-µECD analysis. Optimization of key parameters included types of solid support, and types and volume of the elution solvent. The optimum MSPD condition used 5 g of sorbent 1:1 (w/w) florisil:siliga gel) and 30 mL of hexane to elute twenty six OCPYs. The method has been validated and achieved quantitative analysis down to their maximum residue limit. The linearity range from 0.005-0.50 mg/L, method detection limit is in range of 0.003-0.004 mg/kg and its method quantitation limit is 0.01 mg/kg. Intra –assay precision, intermediate precision and relative standard deviation were less than 7.5 % when compared with the value from Horwitz equation. For all compounds at 0.01, 0.05 and 0.10 mg/kg, recoveries obtained were generally in the range of 83.6-107.0. The method was used to test 4 samples from 2 Thai markets. All of them were contaminated with Cypermethrin in low concentration and lower than MRLs of EU regulations. The approach can be used as a standard method for quantitative analysis of organochlorine and pyrethroid pesticides in Garlic.
DOI: 10.17632/4jc5hz4mp3.1
title: Post-term pregnancy outpatient clinic model

description: A hybrid Discrete Event Simulation, Agent Based model of a post-term pregnancy outpatient clinic model at a large Norwegian hospital.
DOI: 10.17632/9vr5rb99d2.1
title: TCDD & TCDF RNAseq Data

description: Fetal rat exposure to TCDD reduces epididymal sperm number and a proposed mode-of-action (MOA) involves altered pituitary-testicular hormonal signaling. To evaluate this MOA and compare TCDD and TCDF congeners, an in utero rat exposure and evaluation model was used. Endpoints included congener tissue levels and transcriptomes of maternal liver and fetal liver, testis, and pituitary. Decreased gonadotropin subunit mRNAs levels (Lhb and Fshb) and enriched signaling pathways including GNRH Signaling and Calcium Signaling were observed in fetal pituitary after TCDD (but not TCDF) exposure. TCDD (but not TCDF) decreased fetal testis cholesterologenic and steroidogenic pathway genes. TCDD tissue concentrations in dam liver, dam adipose, and whole fetus were approximately 3- to 6-fold higher than TCDF. These results support a MOA for dioxin-induced rat male reproductive toxicity involving key events in both the fetal pituitary (e.g., reduced gonadotropin production) and fetal testis (e.g., reduced Leydig cell cholesterologenesis and steroidogenesis).
DOI: 10.17632/bppgpz9vx8.1
title: Media Coverage of HPV and HPV Vaccine-Related Issues in English Language News Media in India (2015-2018)
DOI: 10.5519/qd.xyi0jmsu
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (9 records)
DOI: 10.7910/dvn/6zxagt/iqlbim
title: .Rbuildignore

description: :unav
DOI: 10.7910/dvn/6zxagt/m5xhcp
title: Dockerfile

description: :unav
DOI: 10.7910/dvn/6zxagt/mjl0qp
title: .gitignore

description: :unav
DOI: 10.14457/cu.the.2010.762
title: Physical property improvement of rubberwood with polymer based on drying oil

description: The effect of heat treatment on rubberwood and preparation of treated rubberwood based on fatty acids in tung oil, linseed oil, sunflower oil, palm oil and epoxidized oil has been studied. The effect of heat treatment at 100-200ºC for time spans ranging 1-8 h on the mass loss (%), the appearance of rubberwood and the physical properties was investigated. Commercial rubberwood and fresh rubberwood were used for this study. Various conditions in preparation process including soaking time, type of oil and treatment process were varied and the water absorption, dimension stability, water contact angle, flexure stress and modulus of elasticity of untreated rubberwood and treated rubberwood were compared. Results showed that higher temperature of heat treatment of rubberwood gave more hydrophobic property. The treated commercial rubberwood at 160ºC and the fresh rubberwood at 180ºC gave water absorption better than other temperatures of the heat treatment and dimension stability of heat treated woods were improved. The oil treatment by impregnation procees with tung oil, linseed oil, sunflower oil, palm oil and epoxidized oil improved the water absorption, volume swelling, water contact angle, flexure stress and modulus of elasticity of rubberwood.
DOI: 10.14457/cu.the.2010.763
title: Effects of hydrophilic content on properties of waterborn polyurethane

description: Polyurethane dispersion containing ionic soft segments (PU-M) was synthesized by modification of the conventional method. The ionic group content was varied by changing the amount of the internal emulsifier—dimethylolpropionic acid (DMPA) from 3 to 7 wt%. Polytetramethylene ether glycol and DMPA were reacted with the first portion of the total isophorone diisocyanate (IPDI) content. Then 1,4-butane diol and the rest of IPDI were added. Finally, the resulting prepolymer was neutralized and dispersed with water. The conventional PU dispersion containing the same ionic content was also synthesized for comparison. All PU dispersions were characterized by FTIR and 1H NMR spectrometer, laser particle size distribution analyzer, differential scanning calorimeter, thermogravimetric analyzer and pencil hardness tester. At the same content of ionic groups, PU-M had smaller particle size and larger contact angle with water. It also had higher Tg and hardness than PU-C. The increase in the ionic group content also gave smaller particle size, higher Tg and higher hardness for both PU-M and PU-C.
DOI: 10.14457/cu.the.2010.764
title: Synthesis of triazines containing porphyrin as photoactive compounds for solar cells

description: Porphyrins as light harvesters in optoelectronic device are useful for their extremely high potential absorption coefficients excellent change mobility, and relative ease with which a variety of covalent and non-covalent bonding possibilities. This research described the synthesis of a series of porphyrinic compounds, investigation of their electrochemical and photophysical properties, and fabrication/evaluation of solar cells based on these compounds. The target molecules include two or three porphyrin macrocycles connected via triazine units. When two porphyrin macrocycles are presented, the remaining peripheral position on the triazine ring is taken by a 4-carboxyphenyl group or 4-(2-carboxy-2-cyanoethenyl)phenyl anchoring group or carbamyl group. Introduction of more than one porphyrin macrocycle enhances the light-harvesting efficiency of these compounds. Triazinesare known to be good charge transporters. Cyclic voltammograms of these compounds were recorded to obtain HOMO-LUMO energy levels, and the film morphology was investigated as well. According to finding from these studies, organic photovoltaic cells were prepared and light-to-electrical energy conversion efficiencies of the resulting devices were evaluated.