DOI: 10.5061/dryad.6h7nt
title: Data from: Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging

description: We report a method for the sensitive detection of rare chromosome breaks
 and translocations in interphase cells. HiBA-FISH (High-throughput
 break-apart FISH) combines high-throughput imaging with the measurement of
 the spatial separation of FISH probes flanking target genome regions of
 interest. As proof-of-principle, we apply hiBA-FISH to detect with high
 sensitivity and specificity rare chromosome breaks and translocations in
 the anaplastic large cell lymphoma breakpoint regions of NPM1 and ALK.
 This method complements existing approaches to detect translocations by
 overcoming the need for precise knowledge of translocation breakpoints and
 it extends traditional FISH by its quantitative nature.
description: ReadmeReadme
 file.Acapella_Scripts_ParametersThis zipped file contains the Acapella script used to analyze all the image datasets and the Acapella parameters files for each analysis session. The parameters files are named according to the relative image dataset analyzed.R_Analysis_Fig3_6This zipped file contains the The the .txt single-object level image analysis results files generated by Acapella, the .Rmd R script used for the analysis and the .html R output file. This .Rmd R script was used to generate Fig. 3 - 6 of the article.R_Analysis_FigS1This zipped file contains the The the .txt single-object level image analysis results files generated by Acapella, the .Rmd R script used for the analysis and the .html R output file. This .Rmd R script was used to generate Fig. S1 of the article.BC_140819_M2A_GFP_ALK_UNThis zipped file contains the image dataset relative to Untreated Mac2A cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. 3 - 6 of the article.BC_140713_K299_K299_ALK_UNThis zipped file contains the image dataset relative to Untreated K299 cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. 3 - 6 of the article.BC_140713_K299_K299_NPM_UNThis zipped file contains the image dataset relative to Untreated K299 cells stained with the hiBA-NPM1 probe set. This dataset was used to generate Fig. 3 - 6 of the article.BC_140815_M2A_GFP_ALK_IRThis zipped file contains the image dataset relative to Irradiated (25 Gy) Mac2A cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. 3 - 6 of the article.BC_140815_M2A_GFP_NPM_IRThis zipped file contains the image dataset relative to Irradiated (25 Gy) Mac2A cells stained with the hiBA-NPM1 probe set. This dataset was used to generate Fig. 3 - 6 of the article.BC_140819_M2A_GFP_NPM_UNThis zipped file contains the image dataset relative to Untreated Mac2A cells stained with the hiBA-NPM1 probe set. This dataset was used to generate Fig. 3 - 6 of the article.BC_150527_K299_ZERO_ALK_UNThis zipped file contains the image dataset relative to 0% Untreated K299 cells (100% Mac2A cells) stained with the hiBA-ALK probe set. This dataset was used to generate Fig. S1 of the article.BC_150527_K299_POINTONE_ALK_UNThis zipped file contains the image dataset relative to 0.1% Untreated K299 cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. S1 of the article.BC_150527_K299_POINTFIVE_ALK_UNThis zipped file contains the image dataset relative to 0.5% Untreated K299 cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. S1 of the article.BC_150527_K299_ONE_ALK_UNThis zipped file contains the image dataset relative to 1% Untreated K299 cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. S1 of the article.BC_150527_K299_TEN_ALK_UNThis zipped file contains the image dataset relative to 10% Untreated K299 cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. S1 of the article.BC_150527_K299_HUNDRED_ALK_UNThis zipped file contains the image dataset relative to 100% Untreated K299 cells stained with the hiBA-ALK probe set. This dataset was used to generate Fig. S1 of the article.