DOI: 10.6084/m9.figshare.9750602
title: Quantitative phase microscopy images of spherical droplets, beads, and cells

description: Example data sets for quantitative phase imaging.<br><br>These data were used by Müller et al. [1] to demonstrate the<br>accurate retrieval of size and refractive index for spherical<br>objects by means of a two-dimensional phase data fitting<br>algorithm.<br><br>Abbreviations:<br><br> - DHM: digital holographic microscopy<br> - FUS: fused in sarcoma (RNA-binding protein)<br> - HL60: name of a human leukemia cell line<br> - QLSI: quadri-wave lateral shearing interferometry<br> - PAA: Polyacrylamide (hydrogel)<br> - PBS: phosphate buffered saline (buffer medium)<br><br>The data are related to [1] according to the following scheme:<br><br>Figure 4:<br><br> a-c) SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07<br> Silicone oil droplet measured in PBS using QLSI<br> d-f) SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5<br> FUS protein droplet measured in egg extract using QLSI<br> (included in fig. 5a)<br> g-j) SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23<br> PAA hydrogel bead (referred to as "type A" in [1]) measured<br> in PBS using QLSI (included in fig. 5b)<br> k-m) DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11<br> PAA hydrogel bead (referred to as "type B" in [1]) measured<br> in PBS using DHM<br> n-q) QLSI_2015-11-09_16-03-52_HL60<br> HL60 cells measured in PBS using QLSI (included in fig. 5c)<br> r-s) DHM_2015-11-09_16-48-45_HL60<br> HL60 cells measured in PBS using DHM (included in fig. 5d)<br><br>Figure 5:<br><br> a) QLSI_2016-04-29_FUS-droplet-780-5<br> Time lapse measurement of a FUS protein droplet measured in<br> egg extract using QLSI<br> b) QLSI_2016-07-05_PAA-beads-S6-A<br> PAA hydrogel beads (referred to as "type A" in [1]) measured<br> in PBS using QLSI<br> c) QLSI_2015-11-09_HL60<br> HL60 cells measured in PBS using QLSI<br> d) DHM_2015-11-09_HL60<br> HL60 cells measured in PBS using DHM<br><br>The data are stored in different file formats:<br><br> - .tif: digital holography data or (!) SID4Bio (Phasics S.A.) data<br> - .h5: HDF5-based qpimage [2] file format<br> - .zip: each ZIP file contains several .tif files<br> - .cfg: meta data and analysis recipe for each data set,<br> stored as a DryMass [3] profile<br><br>The phase data can be extracted with DryMass [3] and qpformat [4],<br>e.g. using the command-line interface of DryMass:<br><br> dm_analyze_sphere --profile DHM_2015-11-09_HL60.cfg DHM_2015-11-09_HL60.zip<br><br>For more information and a detailed analysis, please refer to [1].<br><br><br>License: CC0 (https://creativecommons.org/publicdomain/zero/1.0/)<br><br><br>SHA256 sums:<br><br>cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_16-48-45_HL60.cfg<br>04eaf59da61934486385f42600aec0a2ffb3f71c93c2b6b6f247ac237088eefb DHM_2015-11-09_16-48-45_HL60.tif<br>cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_HL60.cfg<br>64e7b0e6fab4d947b82333ea2edd20b41ce51c813c97ae1070da90e58de35b02 DHM_2015-11-09_HL60.zip<br>685c886d44b9359dca1b2d2bd504f2b1d5f149c05da5a1263ee802c02fb41e66 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.cfg<br>75608e106ecf41a22d707c966faf850f1414e9f0012ce69104658680b4b26876 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.tif<br>138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_16-03-52_HL60.cfg<br>73dd8612b713b62227f52e68619f2e5794adabd29c09622b985fdcc842eb68ff QLSI_2015-11-09_16-03-52_HL60.h5<br>138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_HL60.cfg<br>9f3af6ccdd0a71e53d2907ee8f7b6e478c56da99d3dac9a443aceaa4b9f1658e QLSI_2015-11-09_HL60.h5<br>1a95d8bfcee3e4b442afccd88d0fa89ed9013fe0c96bf5a337ede481eef1f99d QLSI_2016-04-29_FUS-droplet-780-5.cfg<br>f9a407ca31f3aaa106aa7b5db74ab74b9244e9624212e312ce3c82afa60ef714 QLSI_2016-04-29_FUS-droplet-780-5.zip<br>3dc230d213d788d194b053c04ed019104207aa631eac27924215232dce0b0773 QLSI_2016-07-05_PAA-beads-S6-A.cfg<br>7fa1becd016dcd3bf28c8c4c693e15110d239dca803414eed9eb0a62bc5a9daf QLSI_2016-07-05_PAA-beads-S6-A.zip<br>ea8143e609217dc54e2ded3185ff4b88605c995376a4b86cb297a44aa4ae7baf SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.cfg<br>5819ec142b58fd7ef9df614a4ac63c78efdaf9ae9e8f02b5ce2f6215fd9251d1 SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.tif<br>0b478d422d6510aa4cf8b5d082128c595cac4a4d3b1ce4c53c8b496d410299cf SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.cfg<br>ab9503b33562968333423b5a541ee41cac135fa1153a835da3c009f9f5c305ed SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.tif<br>3bb6ed2275f61d03628e5dbea861b7fe87870cd17cd998679cd14da30aaab274 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.cfg<br>e2b3d79bf3b7904a9184b79746dbeff696f716e6789541ff6835672514a25a93 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.tif<br><br><br>References:<br><br>[1] https://dx.doi.org/10.1364/OE.26.010729<br>[2] https://qpimage.readthedocs.io<br>[3] https://drymass.readthedocs.io<br>[4] https://qpformat.readthedocs.io<br><br>