DOI: 10.5061/dryad.2b6b43k
title: Data from: A cost-and-time effective procedure to develop SNP markers for multiple species: a support for community genetics

description: 1.Multi‐species population genetics is an emerging field that provides
 insight relevant to conservation biology and community ecology. However,
 to date, this approach is limited to species with available genetic
 resources. The use of thousands of single nucleotide polymorphism (SNP)
 markers developed from recent genotyping‐by‐sequencing (GBS) technologies
 is a roadmap for the study of non‐model species, but remains cost
 prohibitive when several, distantly related species are involved. 2.We
 aimed to overcome this issue by using a single HiSeq3000 run of
 restriction‐site associated DNA sequencing (RAD‐Seq) to retrieve SNP
 markers for 40 diverse species including plants, invertebrates, fish and
 mammals. We developed a Python‐based pipeline to isolate ~100‐500
 high‐quality SNP markers for each species that could be genotyped through
 classical PCR amplification methods. To assess the quality of these
 markers, we validated our approach on ~160 of the characterized SNPs for
 each of 18 Neotropical fish species from the river Maroni (French Guiana,
 South America), using the MassARRAY iPLEX platform from Agena Bioscience
 (San Diego, CA, USA). 3.A run of the pipeline applying stringent filtering
 parameters enabled the successful design of between 130 and 3492 SNP
 markers for 30 of the 40 study species. Relaxing pipeline parameters
 allows for an increase in the number of detected SNPs. Across the 18
 species from French Guiana, an average of 85% of markers were successfully
 amplified, polymorphic, and scored in ≥90% of individuals (~200
 individuals per species). The great majority (&gt;98%) of these
 markers were at Hardy‐Weinberg equilibrium in each sampling site from the
 river Maroni. 4.This SNP discovery was performed at the cost of ~$US110
 for each of the 40 species. Genotyping was performed at the cost of
 ~$US6000 for each of the 18 fish species with an average of 200
 individuals per species. This strategy was found cost‐and‐time efficient
 to develop hundreds of SNP markers for a large range of non‐model species,
 which can be used to investigate ecological and evolutionary questions
 that do not require whole‐genome coverage.
description: Fasta_Pipelineout_FG_fish_speciesThis repository contains one fasta file per fish species from French Guiana (= 18 fasta files). Each file contains the list of the SNP markers that were generated as output from our custom pipeline for the corresponding species. Each file has been used as a template to build SNP multiplexes for further MassArray genotyping. The molecular resources provided here were developed from samples collected in collaboration with the National Amazonian Park in French Guiana, under the contract R&D_2003_06 and with ethical consideration defined in the convention APA-973-7.Fasta_Pipelineout_nonFG_speciesThis repository contains one fasta file per species from the study from Delord et al. 2018, except for fish from French Guiana (= 22 fasta files). Each file contains the list of the SNP markers that were generated as output from our custom pipeline for the corresponding species, and could be used as a template for amplification-based genotyping.SNPs_Validation_FG_fish_speciesThis file contains information about SNPs genotyped with the MassARRAY technology for each of 18 fish species from French Guiana. The composition of the four SNP multiplexes built for each species, primer pairs, and the list of successfully genotyped markers are provided. The molecular resources provided here were developed from samples collected in collaboration with the National Amazonian Park in French Guiana, under the contract R&D_2003_06 and with ethical consideration defined in the convention APA-973-7.FG_fish_species_GenotypesThis file contains the individual genotypes table for each of 18 species from French Guiana; and the 111 to 156 SNP markers that were validated through MassARRAY procedure. The molecular resources provided here were developed from samples collected in collaboration with the National Amazonian Park in French Guiana, under the contract R&D_2003_06 and with ethical consideration defined in the convention APA-973-7.FG_FishSpecies_Genotypes.xlsx