cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.35077/net
title: 598093

description: This is a series of monosynaptic, retrograde trans-synaptic tracing images of whole mouse brain to map whole-brain inputs to genetically-identified cell populations brain-wide.
description: DATASET METADATA: title:'598093', GeneralModality:'connectivity', Technique:'retrograde transsynaptic tracing' SPECIMEN METADATA: LocalID:'598093', Species:'Mouse', NCBITaxonomy:'NCBI:txid10090', Age:'93.0', Ageunit:'Days', Sex:'Female', Genotype:'Penk-IRES2-Cre-neo/wt', OrganLocalID:'1134344914.0', OrganName:'Brain', SampleLocalID:'0539072403' INSTRUMENT METADATA: MicroscopeType:'Two Photon', MicroscopeManufacturerAndModel:'TissueVision TissueCyte 1000', ObjectiveName:'Zeiss 20X Plan-Apochromat', ObjectiveImmersion:'Water', ObjectiveNA:'1.0', ObjectiveMagnification:'20X', DetectorType:'PMT', DetectorModel:'Hamamatsu R3896', IlluminationWavelength:'925nm', DetectionWavelength:'447-593nm', SampleTemperature:'20.0' IMAGE METADATA: xAxis:'left-to-right', yAxis:'superior-to-inferior', zAxis:'posterior-to-anterior', Number:'1,2,3,red,green,blue', displayColor:'(1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0)', Representation:'neurite', Flurophore:'CH1(background fluorescence) CH2(GFP) CH3(background fluorescence)', stepSizeX:'0.875', stepSizeY:'0.875', stepSizeZ:'100.0', Channels:'3.0', Slices:'140.0', z:'1.0', Xsize:'16000.0', Ysize:'12000.0', Zsize:'1.0'
DOI: 10.6084/m9.figshare.19905503
title: Quantification and conceptual validation of the inoculum potential of Sclerotinia sclerotiorum in soybean and bean seeds

description: Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout the world. The disease is considered to be one of the major diseases of soybean and bean crops in Brazil. The pathogen S. sclerotiorum is spread by soybean and bean seeds both in the form of sclerotia and dormant mycelium inside the seeds. The objective of this work was to evaluate the relationship between different potentials of S. sclerotiorum in soybean and bean seeds and the performance of these seeds, as well as to verify the localization and quantification of the inoculum of the pathogen in the seeds inoculated by Real-time PCR (qPCR), validating the term inoculum potential. Soybean and bean seeds were inoculated with the fungus by the osmotic conditioning method based on the exposure of the seeds to the fungus for periods of 24 h, 48 h, 72 h, and 96 h. Molecular analysis was carried out by means of qPCR in whole seeds and dissected in the integument, cotyledon and embryonic axis. The results showed that the effects of S. sclerotiorum on seed germination and vigor were progressive and proportional to the increases in inoculum potentials, since there was more severe damage to the seeds and consequently to the emerged plants at the highest potential (P96). The inoculum of the pathogen was found in all parts of the evaluated seeds, even at its lowest inoculum potential (P24), with an increasing DNA concentration, and the integument obtained a greater amount of DNA than the embryo, in comparison.
DOI: 10.6084/m9.figshare.19905503.v1
title: Quantification and conceptual validation of the inoculum potential of Sclerotinia sclerotiorum in soybean and bean seeds

description: Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout the world. The disease is considered to be one of the major diseases of soybean and bean crops in Brazil. The pathogen S. sclerotiorum is spread by soybean and bean seeds both in the form of sclerotia and dormant mycelium inside the seeds. The objective of this work was to evaluate the relationship between different potentials of S. sclerotiorum in soybean and bean seeds and the performance of these seeds, as well as to verify the localization and quantification of the inoculum of the pathogen in the seeds inoculated by Real-time PCR (qPCR), validating the term inoculum potential. Soybean and bean seeds were inoculated with the fungus by the osmotic conditioning method based on the exposure of the seeds to the fungus for periods of 24 h, 48 h, 72 h, and 96 h. Molecular analysis was carried out by means of qPCR in whole seeds and dissected in the integument, cotyledon and embryonic axis. The results showed that the effects of S. sclerotiorum on seed germination and vigor were progressive and proportional to the increases in inoculum potentials, since there was more severe damage to the seeds and consequently to the emerged plants at the highest potential (P96). The inoculum of the pathogen was found in all parts of the evaluated seeds, even at its lowest inoculum potential (P24), with an increasing DNA concentration, and the integument obtained a greater amount of DNA than the embryo, in comparison.
DOI: 10.6084/m9.figshare.19905504
title: Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

description: Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.
DOI: 10.6084/m9.figshare.19905504.v1
title: Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

description: Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.
DOI: 10.35077/new
title: 598761

description: This is a series of monosynaptic, retrograde trans-synaptic tracing images of whole mouse brain to map whole-brain inputs to genetically-identified cell populations brain-wide.
description: DATASET METADATA: title:'598761', GeneralModality:'connectivity', Technique:'retrograde transsynaptic tracing' SPECIMEN METADATA: LocalID:'598761', Species:'Mouse', NCBITaxonomy:'NCBI:txid10090', Age:'87.0', Ageunit:'Days', Sex:'Female', Genotype:'Penk-IRES2-Cre-neo/wt', OrganLocalID:'1134344908.0', OrganName:'Brain', SampleLocalID:'0539070482' INSTRUMENT METADATA: MicroscopeType:'Two Photon', MicroscopeManufacturerAndModel:'TissueVision TissueCyte 1000', ObjectiveName:'Zeiss 20X Plan-Apochromat', ObjectiveImmersion:'Water', ObjectiveNA:'1.0', ObjectiveMagnification:'20X', DetectorType:'PMT', DetectorModel:'Hamamatsu R3896', IlluminationWavelength:'925nm', DetectionWavelength:'447-593nm', SampleTemperature:'20.0' IMAGE METADATA: xAxis:'left-to-right', yAxis:'superior-to-inferior', zAxis:'posterior-to-anterior', Number:'1,2,3,red,green,blue', displayColor:'(1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0)', Representation:'neurite', Flurophore:'CH1(background fluorescence) CH2(GFP) CH3(background fluorescence)', stepSizeX:'0.875', stepSizeY:'0.875', stepSizeZ:'100.0', Channels:'3.0', Slices:'140.0', z:'1.0', Xsize:'16000.0', Ysize:'12000.0', Zsize:'1.0'
DOI: 10.6084/m9.figshare.19905506
title: Biochar as an alternative substrate for the production of sugarcane seedlings

description: ABSTRACT Biochar, which has emerged as an important form of the transformation and final disposal of biomass, can be used directly in soil or in seedling nurseries. In this study, the use of biochar of different particle sizes and percentages was evaluated in replacement to a conventional substrate used in the production of sugarcane seedlings. To this end, an experiment was carried out based on a completely randomized design, with a 5 × 4 factorial scheme, consisting of five different percentages of biochar (with 0, 25, 50, 75, and 100% v/v substitution of the conventional substrate) and four particle sizes (&lt;1, 2, 4, and 9 mm), with nine repetitions. As seedling growth variables, the average sprouting time, sprouting speed index, plant height, leaf number, leaf length, and width + 2, as well as the dry mass of the aerial parts and roots were evaluated. Irrespective of the percentage of commercial substrate replaced with biochar, sprouting time was found to be shorter when 6-mm-diameter biochar particles were used. With respect to the sprouting speed index, it was found that regardless of particle size, the highest value occurred when biochar was used to replace 42% of the commercial substrate. The substitution of the commercial substrate with biochar had the effect of reducing the growth of sugarcane seedlings.
DOI: 10.6084/m9.figshare.19905506.v1
title: Biochar as an alternative substrate for the production of sugarcane seedlings

description: ABSTRACT Biochar, which has emerged as an important form of the transformation and final disposal of biomass, can be used directly in soil or in seedling nurseries. In this study, the use of biochar of different particle sizes and percentages was evaluated in replacement to a conventional substrate used in the production of sugarcane seedlings. To this end, an experiment was carried out based on a completely randomized design, with a 5 × 4 factorial scheme, consisting of five different percentages of biochar (with 0, 25, 50, 75, and 100% v/v substitution of the conventional substrate) and four particle sizes (&lt;1, 2, 4, and 9 mm), with nine repetitions. As seedling growth variables, the average sprouting time, sprouting speed index, plant height, leaf number, leaf length, and width + 2, as well as the dry mass of the aerial parts and roots were evaluated. Irrespective of the percentage of commercial substrate replaced with biochar, sprouting time was found to be shorter when 6-mm-diameter biochar particles were used. With respect to the sprouting speed index, it was found that regardless of particle size, the highest value occurred when biochar was used to replace 42% of the commercial substrate. The substitution of the commercial substrate with biochar had the effect of reducing the growth of sugarcane seedlings.
DOI: 10.6084/m9.figshare.19905507
title: Methodological adjustments to the tetrazolium test in coriander seeds

description: Abstract: The reduction in the time to perform the tetrazolium test is critical in decision making at pre and post-harvest of seeds. Thus, the objective of this study was to investigate different concentrations of the tetrazolium salt solution, temperatures, and staining times, to reduce the time for assessing the viability of coriander seeds by the tetrazolium test. The study was divided into two experiments conducted independently but sequentially. The seeds were evaluated for water content, germination, and viability in both experiments by the tetrazolium test. A completely randomized design was used, with four replications. For the tetrazolium test, the seeds were preconditioned by direct immersion in water for sixteen hours at 20 °C. In experiment I, the seeds were exposed to different concentrations of the tetrazolium salt solution (0.25; 0.5; 0.75; and 1.0%). In experiment II, different combinations of temperatures (35; 40; and 45 °C) and staining times (three, four, five, and six hours) were tested. Based on the findings, it was concluded that the best staining pattern of coriander seeds for performing the tetrazolium test is achieved by using the concentration of 0.5% tetrazolium salt solution for five hours at 40 °C.
DOI: 10.6084/m9.figshare.19905507.v1
title: Methodological adjustments to the tetrazolium test in coriander seeds

description: Abstract: The reduction in the time to perform the tetrazolium test is critical in decision making at pre and post-harvest of seeds. Thus, the objective of this study was to investigate different concentrations of the tetrazolium salt solution, temperatures, and staining times, to reduce the time for assessing the viability of coriander seeds by the tetrazolium test. The study was divided into two experiments conducted independently but sequentially. The seeds were evaluated for water content, germination, and viability in both experiments by the tetrazolium test. A completely randomized design was used, with four replications. For the tetrazolium test, the seeds were preconditioned by direct immersion in water for sixteen hours at 20 °C. In experiment I, the seeds were exposed to different concentrations of the tetrazolium salt solution (0.25; 0.5; 0.75; and 1.0%). In experiment II, different combinations of temperatures (35; 40; and 45 °C) and staining times (three, four, five, and six hours) were tested. Based on the findings, it was concluded that the best staining pattern of coriander seeds for performing the tetrazolium test is achieved by using the concentration of 0.5% tetrazolium salt solution for five hours at 40 °C.
DOI: 10.35077/nil
title: 598671

description: This is a series of monosynaptic, retrograde trans-synaptic tracing images of whole mouse brain to map whole-brain inputs to genetically-identified cell populations brain-wide.
description: DATASET METADATA: title:'598671', GeneralModality:'connectivity', Technique:'retrograde transsynaptic tracing' SPECIMEN METADATA: LocalID:'598671', Species:'Mouse', NCBITaxonomy:'NCBI:txid10090', Age:'87.0', Ageunit:'Days', Sex:'Female', Genotype:'Sepw1-Cre_NP39/wt', OrganLocalID:'1134915536.0', OrganName:'Brain', SampleLocalID:'0539070486' INSTRUMENT METADATA: MicroscopeType:'Two Photon', MicroscopeManufacturerAndModel:'TissueVision TissueCyte 1000', ObjectiveName:'Zeiss 20X Plan-Apochromat', ObjectiveImmersion:'Water', ObjectiveNA:'1.0', ObjectiveMagnification:'20X', DetectorType:'PMT', DetectorModel:'Hamamatsu R3896', IlluminationWavelength:'925nm', DetectionWavelength:'447-593nm', SampleTemperature:'20.0' IMAGE METADATA: xAxis:'left-to-right', yAxis:'superior-to-inferior', zAxis:'posterior-to-anterior', Number:'1,2,3,red,green,blue', displayColor:'(1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0)', Representation:'neurite', Flurophore:'CH1(background fluorescence) CH2(GFP) CH3(background fluorescence)', stepSizeX:'0.875', stepSizeY:'0.875', stepSizeZ:'100.0', Channels:'3.0', Slices:'140.0', z:'1.0', Xsize:'16000.0', Ysize:'12000.0', Zsize:'1.0'
DOI: 10.6084/m9.figshare.19905510
title: Seed characterization and germination morpho-physiology of Simira gardneriana (Rubiaceae)

description: Abstract: Simira gardneriana (Rubiaceae) is a newly discovered tree species endemic to the Caatinga. For this reason, studies aimed at propagation, seed and seedlings analysis are essential for morphology, identification, classification, and conservation. Our objective was to characterize the seeds, the morpho-physiology of germination and seedling establishment of S. gardneriana. Therefore, we performed physical, biometric, biochemical, and morphological characterization of the seeds, as well as an evaluation of the germination process and seedling establishment. Hydration of seeds was carried out by three methods (immersion in water, on a wire mesh, and between paper rolls) and at two temperatures (25 and 30 °C). Morphological characterization of the seedlings was performed on paper substrate at 30 °C for 28 days. Seeds of S. gardneriana are orthodox, amylo-lipid, have an endosperm and do not exhibit physical dormancy. Hydration and germination of the seeds can be achieved in a paper roll at 30 °C with root protrusion on the sixth day, and complete seedling formation on the 28th day after sowing. Seedlings of S. gardneriana are phanero-epigeal cotyledonous with photosynthetic cotyledons and have ability to regrow the root system. Characterization of S. gardneriana seeds, germination and seedlings contributes to its propagation and may help in subsequent taxonomic and phylogenetic studies in Rubiaceae.
DOI: 10.6084/m9.figshare.19905510.v1
title: Seed characterization and germination morpho-physiology of Simira gardneriana (Rubiaceae)

description: Abstract: Simira gardneriana (Rubiaceae) is a newly discovered tree species endemic to the Caatinga. For this reason, studies aimed at propagation, seed and seedlings analysis are essential for morphology, identification, classification, and conservation. Our objective was to characterize the seeds, the morpho-physiology of germination and seedling establishment of S. gardneriana. Therefore, we performed physical, biometric, biochemical, and morphological characterization of the seeds, as well as an evaluation of the germination process and seedling establishment. Hydration of seeds was carried out by three methods (immersion in water, on a wire mesh, and between paper rolls) and at two temperatures (25 and 30 °C). Morphological characterization of the seedlings was performed on paper substrate at 30 °C for 28 days. Seeds of S. gardneriana are orthodox, amylo-lipid, have an endosperm and do not exhibit physical dormancy. Hydration and germination of the seeds can be achieved in a paper roll at 30 °C with root protrusion on the sixth day, and complete seedling formation on the 28th day after sowing. Seedlings of S. gardneriana are phanero-epigeal cotyledonous with photosynthetic cotyledons and have ability to regrow the root system. Characterization of S. gardneriana seeds, germination and seedlings contributes to its propagation and may help in subsequent taxonomic and phylogenetic studies in Rubiaceae.
DOI: 10.6084/m9.figshare.19905516
title: Growth and physiology of ornamental sunflower under salinity in function of paclobutrazol application methods

description: ABSTRACT The ornamental sunflower has great importance in the floriculture sector due to the color and vitality of its flowers. However, the production and quality of flowers decrease under salt stress, which can be mitigated with paclobutrazol application. The objective of the present study was to evaluate the effects of different application methods of paclobutrazol in ornamental sunflower ‘Sol Noturno’ irrigated with brackish waters. The experimental design was randomized blocks arranged in a 5 × 3 factorial scheme, corresponding to five electrical conductivities of irrigation water - ECw (0.4; 1.9; 3.4; 4.9, and 6.4 dS m-1) and three paclobutrazol application methods (foliar application, via soil and a control treatment - without paclobutrazol), with four replicates. The increase in salinity of irrigation water reduced gas exchange, photosystem II photochemical efficiency, SPAD index, plant height, and chapter diameter. The paclobutrazol application via soil or foliar increased stomatal conductance and transpiration by 21.09 and 17.80%, respectively, in comparison to plants without application, whereas photosynthesis and instantaneous carboxylation efficiency increased by 28.33 and 31.18% via soil and 14.40 and 16.12% via foliar, respectively. The paclobutrazol application, mainly via soil, favored ‘Sol Noturno’ sunflower plants under salt stress, increasing chlorophyll SPAD index and external chapter diameter, and keeping the number of the petals.
DOI: 10.6084/m9.figshare.19905516.v1
title: Growth and physiology of ornamental sunflower under salinity in function of paclobutrazol application methods

description: ABSTRACT The ornamental sunflower has great importance in the floriculture sector due to the color and vitality of its flowers. However, the production and quality of flowers decrease under salt stress, which can be mitigated with paclobutrazol application. The objective of the present study was to evaluate the effects of different application methods of paclobutrazol in ornamental sunflower ‘Sol Noturno’ irrigated with brackish waters. The experimental design was randomized blocks arranged in a 5 × 3 factorial scheme, corresponding to five electrical conductivities of irrigation water - ECw (0.4; 1.9; 3.4; 4.9, and 6.4 dS m-1) and three paclobutrazol application methods (foliar application, via soil and a control treatment - without paclobutrazol), with four replicates. The increase in salinity of irrigation water reduced gas exchange, photosystem II photochemical efficiency, SPAD index, plant height, and chapter diameter. The paclobutrazol application via soil or foliar increased stomatal conductance and transpiration by 21.09 and 17.80%, respectively, in comparison to plants without application, whereas photosynthesis and instantaneous carboxylation efficiency increased by 28.33 and 31.18% via soil and 14.40 and 16.12% via foliar, respectively. The paclobutrazol application, mainly via soil, favored ‘Sol Noturno’ sunflower plants under salt stress, increasing chlorophyll SPAD index and external chapter diameter, and keeping the number of the petals.
DOI: 10.35077/nip
title: 599648

description: This is a series of monosynaptic, retrograde trans-synaptic tracing images of whole mouse brain to map whole-brain inputs to genetically-identified cell populations brain-wide.
description: DATASET METADATA: title:'599648', GeneralModality:'connectivity', Technique:'retrograde transsynaptic tracing' SPECIMEN METADATA: LocalID:'599648', Species:'Mouse', NCBITaxonomy:'NCBI:txid10090', Age:'79.0', Ageunit:'Days', Sex:'Female', Genotype:'Sst-IRES-Cre/wt', OrganLocalID:'1135871702.0', OrganName:'Brain', SampleLocalID:'0539072401' INSTRUMENT METADATA: MicroscopeType:'Two Photon', MicroscopeManufacturerAndModel:'TissueVision TissueCyte 1000', ObjectiveName:'Zeiss 20X Plan-Apochromat', ObjectiveImmersion:'Water', ObjectiveNA:'1.0', ObjectiveMagnification:'20X', DetectorType:'PMT', DetectorModel:'Hamamatsu R3896', IlluminationWavelength:'925nm', DetectionWavelength:'447-593nm', SampleTemperature:'20.0' IMAGE METADATA: xAxis:'left-to-right', yAxis:'superior-to-inferior', zAxis:'posterior-to-anterior', Number:'1,2,3,red,green,blue', displayColor:'(1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0)', Representation:'neurite', Flurophore:'CH1(background fluorescence) CH2(GFP) CH3(background fluorescence)', stepSizeX:'0.875', stepSizeY:'0.875', stepSizeZ:'100.0', Channels:'3.0', Slices:'140.0', z:'1.0', Xsize:'16000.0', Ysize:'12000.0', Zsize:'1.0'