cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5772666.v1
title: A non-living, effective model for microvascular training

description: Abstract Purpose: To introduce a nonliving microvascular training model based on vessels diameter and feasibility. Methods: We dissected ten oxen tongues, and divided the pedicles into three-thirds: proximal, middle and distal. We measured the external vessels diameter in all regions. We performed a descriptive statistical analysis. Three students (two beginner level and one intermediate level) performed this training. We evaluated the confidence, according Likert scale. Results: We dissected all oxen tongues, each tongue showed two parallel pedicles. Each pedicle was located at 1.5 - 2.0 cm from the midline. Proximal median artery and vein diameter were 3.9 ± 0.7, and 5.04 ± 1.44mm, respectively. In the middle third, the mean artery diameter was 3.3 ± 0.4mm, and the vein diameter was 3.5 ± 0.9mm. The distal third showed a mean artery diameter of 2.0 ± 0.42mm, and a vein diameter of 2.4 ± 0.82mm. The students performed ten anastomoses. This study showed a higher confidence level (CL) (p=0.03) than the pre training CL assessment. Conclusion: This study suggested a feasible non-animal model for microsurgical training process for beginners and intermediate trainees.
DOI: 10.6084/m9.figshare.5772660
title: SPECT/CT with radiolabeled somatostatin analogues in the evaluation of systemic granulomatous infections

description: Abstract Objective: To evaluate SPECT/CT with radiolabeled somatostatin analogues (RSAs) in systemic granulomatous infections in comparison with gallium-67 (67Ga) citrate scintigraphy. Materials and Methods: We studied 28 patients with active systemic granulomatous infections, including tuberculosis, paracoccidioidomycosis, pneumocystosis, cryptococcosis, aspergillosis, leishmaniasis, infectious vasculitis, and an unspecified opportunistic infection. Of the 28 patients, 23 had started specific treatment before the study outset. All patients underwent whole-body SPECT/CT imaging: 7 after injection of 99mTc-EDDA-HYNIC-TOC, and 21 after injection of 111In-DTPA-octreotide. All patients also underwent 67Ga citrate imaging, except for one patient who died before the 67Ga was available. Results: In 20 of the 27 patients who underwent imaging with both tracers, 27 sites of active disease were detected by 67Ga citrate imaging and by SPECT/CT with an RSA. Both tracers had negative results in the other 7 patients. RSA uptake was visually lower than 67Ga uptake in 11 of the 20 patients with positive images and similar to 67Ga uptake in the other 9 patients. The only patient who did not undergo 67Ga scintigraphy underwent 99mTc-EDDA-HYNIC-TOC SPECT/CT-guided biopsy of a lung cavity with focal RSA uptake, which turned to be positive for aspergillosis. Conclusion: SPECT/CT with 99mTc-EDDA-HYNIC-TOC or 111In-DTPA-octreotide seems to be a good alternative to 67Ga citrate imaging for the evaluation of patients with systemic granulomatous disease.
DOI: 10.6084/m9.figshare.5772660.v1
title: SPECT/CT with radiolabeled somatostatin analogues in the evaluation of systemic granulomatous infections

description: Abstract Objective: To evaluate SPECT/CT with radiolabeled somatostatin analogues (RSAs) in systemic granulomatous infections in comparison with gallium-67 (67Ga) citrate scintigraphy. Materials and Methods: We studied 28 patients with active systemic granulomatous infections, including tuberculosis, paracoccidioidomycosis, pneumocystosis, cryptococcosis, aspergillosis, leishmaniasis, infectious vasculitis, and an unspecified opportunistic infection. Of the 28 patients, 23 had started specific treatment before the study outset. All patients underwent whole-body SPECT/CT imaging: 7 after injection of 99mTc-EDDA-HYNIC-TOC, and 21 after injection of 111In-DTPA-octreotide. All patients also underwent 67Ga citrate imaging, except for one patient who died before the 67Ga was available. Results: In 20 of the 27 patients who underwent imaging with both tracers, 27 sites of active disease were detected by 67Ga citrate imaging and by SPECT/CT with an RSA. Both tracers had negative results in the other 7 patients. RSA uptake was visually lower than 67Ga uptake in 11 of the 20 patients with positive images and similar to 67Ga uptake in the other 9 patients. The only patient who did not undergo 67Ga scintigraphy underwent 99mTc-EDDA-HYNIC-TOC SPECT/CT-guided biopsy of a lung cavity with focal RSA uptake, which turned to be positive for aspergillosis. Conclusion: SPECT/CT with 99mTc-EDDA-HYNIC-TOC or 111In-DTPA-octreotide seems to be a good alternative to 67Ga citrate imaging for the evaluation of patients with systemic granulomatous disease.
DOI: 10.6084/m9.figshare.5772654
title: Which is best method for instillation of topical therapy to the upper urinary tract? An in vivo porcine study to evaluate three delivery methods

description: ABSTRACT Purpose: To compare the staining intensity of the upper urinary tract (UUT) urothelium among three UUT delivery methods in an in vivo porcine model. Materials and methods: A fluorescent dye solution (indigo carmine) was delivered to the UUT via three different methods: antegrade perfusion, vesico-ureteral reflux via in-dwelling ureteric stent and retrograde perfusion via a 5F open-ended ureteral catheter. Twelve renal units were tested with 4 in each method. After a 2-hour delivery time, the renal-ureter units were harvested en bloc. Time from harvesting to analysis was also standardised to be 2 hours in each arm. Three urothelium samples of the same weight and size were taken from each of the 6 pre-defined points (upper pole, mid pole, lower pole, renal pelvis, mid ureter and distal ureter) and the amount of fluorescence was measured with a spectrometer. Results: The mean fluorescence detected at all 6 predefined points of the UUT urothelium was the highest for the retrograde method. This was statistically significant with p-value less than &lt;0.05 at all 6 points. Conclusions: Retrograde infusion of UUT by an open ended ureteral catheter resulted in highest mean fluorescence detected at all 6 pre-defined points of the UUT urothelium compared to antegrade infusion and vesico-ureteral reflux via indwelling ureteric stents indicating retrograde method ideal for topical therapy throughout the UUT urothelium. More clinical studies are needed to demonstrate if retrograde method could lead to better clinical outcomes compared to the other two methods.
DOI: 10.6084/m9.figshare.5772654.v1
title: Which is best method for instillation of topical therapy to the upper urinary tract? An in vivo porcine study to evaluate three delivery methods

description: ABSTRACT Purpose: To compare the staining intensity of the upper urinary tract (UUT) urothelium among three UUT delivery methods in an in vivo porcine model. Materials and methods: A fluorescent dye solution (indigo carmine) was delivered to the UUT via three different methods: antegrade perfusion, vesico-ureteral reflux via in-dwelling ureteric stent and retrograde perfusion via a 5F open-ended ureteral catheter. Twelve renal units were tested with 4 in each method. After a 2-hour delivery time, the renal-ureter units were harvested en bloc. Time from harvesting to analysis was also standardised to be 2 hours in each arm. Three urothelium samples of the same weight and size were taken from each of the 6 pre-defined points (upper pole, mid pole, lower pole, renal pelvis, mid ureter and distal ureter) and the amount of fluorescence was measured with a spectrometer. Results: The mean fluorescence detected at all 6 predefined points of the UUT urothelium was the highest for the retrograde method. This was statistically significant with p-value less than &lt;0.05 at all 6 points. Conclusions: Retrograde infusion of UUT by an open ended ureteral catheter resulted in highest mean fluorescence detected at all 6 pre-defined points of the UUT urothelium compared to antegrade infusion and vesico-ureteral reflux via indwelling ureteric stents indicating retrograde method ideal for topical therapy throughout the UUT urothelium. More clinical studies are needed to demonstrate if retrograde method could lead to better clinical outcomes compared to the other two methods.
DOI: 10.6084/m9.figshare.5772642
title: Management of agricultural use of sewage sludge: case study of Paraná state, Brazil

description: ABSTRACT The agricultural use is an environmentally sustainable destination for sewage sludge because it promotes the recycling of nutrients and is beneficial to the cultivation of plants and physicochemical and biological soil characteristics. It is a worldwide consolidated alternative, which in Brazil is limited to a few states. Paraná is one of the states where the sewage sludge has agriculture as a priority disposal. This study aimed to evaluate the management of the agricultural use of sewage sludge process in the state of Paraná, Brazil, in the 2011-2013 period. There were analyzed 49 traceability reports, 337 agronomic projects and laboratory reports of 129 batches of sewage sludge. It was found that 107,416 t of sewage sludge hygienised by prolonged alkaline stabilization were applied on 5,529 ha of agricultural land in Paraná, with the Curitiba Metropolitan Region (CMR) responsible for 54% of the destination. Although the state has a greater potential of agricultural areas for the application of sewage sludge, compared to CMR, the expansion of activity in the interior of Paraná is still modest, with only 22% of the total of 81 management units licensed in the state destining the sludge for agricultural use in 2013.
DOI: 10.6084/m9.figshare.5772642.v1
title: Management of agricultural use of sewage sludge: case study of Paraná state, Brazil

description: ABSTRACT The agricultural use is an environmentally sustainable destination for sewage sludge because it promotes the recycling of nutrients and is beneficial to the cultivation of plants and physicochemical and biological soil characteristics. It is a worldwide consolidated alternative, which in Brazil is limited to a few states. Paraná is one of the states where the sewage sludge has agriculture as a priority disposal. This study aimed to evaluate the management of the agricultural use of sewage sludge process in the state of Paraná, Brazil, in the 2011-2013 period. There were analyzed 49 traceability reports, 337 agronomic projects and laboratory reports of 129 batches of sewage sludge. It was found that 107,416 t of sewage sludge hygienised by prolonged alkaline stabilization were applied on 5,529 ha of agricultural land in Paraná, with the Curitiba Metropolitan Region (CMR) responsible for 54% of the destination. Although the state has a greater potential of agricultural areas for the application of sewage sludge, compared to CMR, the expansion of activity in the interior of Paraná is still modest, with only 22% of the total of 81 management units licensed in the state destining the sludge for agricultural use in 2013.
DOI: 10.6084/m9.figshare.5772639
title: Diabetes mellitus and hyperkalemic renal tubular acidosis: case reports and literature review

description: ABSTRACT Hyporeninemic hypoaldosteronism, despite being common, remains an underdiagnosed entity that is more prevalent in patients with diabetes mellitus. It presents with asymptomatic hyperkalemia along with hyperchloraemic metabolic acidosis without significant renal function impairment. The underlying pathophysiological mechanism is not fully understood, but it is postulated that either aldosterone deficiency (hyporeninemic hypoaldosteronism) and/or target organ aldosterone resistance (pseudohypoaldosteronism) may be responsible. Diagnosis is based on laboratory parameters. Treatment strategy varies according to the underlying pathophysiological mechanism and etiology and aims to normalize serum potassium. Two clínical cases are reported and the relevant literature is revisited.
DOI: 10.6084/m9.figshare.5772639.v1
title: Diabetes mellitus and hyperkalemic renal tubular acidosis: case reports and literature review

description: ABSTRACT Hyporeninemic hypoaldosteronism, despite being common, remains an underdiagnosed entity that is more prevalent in patients with diabetes mellitus. It presents with asymptomatic hyperkalemia along with hyperchloraemic metabolic acidosis without significant renal function impairment. The underlying pathophysiological mechanism is not fully understood, but it is postulated that either aldosterone deficiency (hyporeninemic hypoaldosteronism) and/or target organ aldosterone resistance (pseudohypoaldosteronism) may be responsible. Diagnosis is based on laboratory parameters. Treatment strategy varies according to the underlying pathophysiological mechanism and etiology and aims to normalize serum potassium. Two clínical cases are reported and the relevant literature is revisited.
DOI: 10.6084/m9.figshare.5772633
title: Validation of a forecast system for onion downy mildew

description: ABSTRACT Aimed at validating a forecast system with different levels of daily severity values, compared to conventional spraying to control onion downy mildew, experiments were conducted in Rio do Sul, Santa Catarina State (SC), during the crop seasons of 2014, 2015 and 2016. Spraying regimes were established according to the daily severity values (DSV) of Wallin system (1962), assigning accumulated values of 6, 8, 10 and 12 DSV, and in the conventional system with spraying at every 5 and 7 days, compared to the untreated control. Except for the control, there was no significant difference among treatments for productivity in all cycles. The area under the disease progress curve (AUDPC), the final severity and the disease progression rate did not differ among treatments, but in the forecast system with DSV of 12, the spraying number was 42, 30 and 40% lower in the three years of evaluation, respectively, relative to the weekly application system.
DOI: 10.6084/m9.figshare.5772633.v1
title: Validation of a forecast system for onion downy mildew

description: ABSTRACT Aimed at validating a forecast system with different levels of daily severity values, compared to conventional spraying to control onion downy mildew, experiments were conducted in Rio do Sul, Santa Catarina State (SC), during the crop seasons of 2014, 2015 and 2016. Spraying regimes were established according to the daily severity values (DSV) of Wallin system (1962), assigning accumulated values of 6, 8, 10 and 12 DSV, and in the conventional system with spraying at every 5 and 7 days, compared to the untreated control. Except for the control, there was no significant difference among treatments for productivity in all cycles. The area under the disease progress curve (AUDPC), the final severity and the disease progression rate did not differ among treatments, but in the forecast system with DSV of 12, the spraying number was 42, 30 and 40% lower in the three years of evaluation, respectively, relative to the weekly application system.
DOI: 10.6084/m9.figshare.5772630
title: Linear relationship between leaf incidence and severity of onion downy mildew

description: ABSTRACT Occurrence of onion downy mildew can reduce the productivity and compromise the bulb quality. The aim of this study was to evaluate the relationship between incidence and severity for the intensity of onion downy mildew. The experiment was conducted with cultivar EMPASC 352 – “Bola Precoce” at Catarinense Federal Institute/Rio do Sul Campus. The design was in randomized blocks, including four replicates and six treatments consisting of fungicide spraying according to the forecasting system proposed by Wallin (1962), with accumulation of 6, 8, 10, 12 daily severity values (DSV), compared to application at every 5 and 7 days and control without spraying, in order to generate a disease intensity gradient for 2014 and 2015. The disease intensity was weekly evaluated based on the severity and incidence. The obtained data underwent linear regression analysis. Using the equation that best fit in the two seasons and replacing the severity value of 1%, incidence of 5.7% was obtained. Severity estimated based on incidence and vice versa can be used by technical assistance in the monitoring of onion downy mildew. As the incidence evaluation is more rapid, more accurate and reproducible, according to the obtained data, severity can be estimated based on incidence, facilitating downy mildew quantification by technical assistance.
DOI: 10.6084/m9.figshare.5772630.v1
title: Linear relationship between leaf incidence and severity of onion downy mildew

description: ABSTRACT Occurrence of onion downy mildew can reduce the productivity and compromise the bulb quality. The aim of this study was to evaluate the relationship between incidence and severity for the intensity of onion downy mildew. The experiment was conducted with cultivar EMPASC 352 – “Bola Precoce” at Catarinense Federal Institute/Rio do Sul Campus. The design was in randomized blocks, including four replicates and six treatments consisting of fungicide spraying according to the forecasting system proposed by Wallin (1962), with accumulation of 6, 8, 10, 12 daily severity values (DSV), compared to application at every 5 and 7 days and control without spraying, in order to generate a disease intensity gradient for 2014 and 2015. The disease intensity was weekly evaluated based on the severity and incidence. The obtained data underwent linear regression analysis. Using the equation that best fit in the two seasons and replacing the severity value of 1%, incidence of 5.7% was obtained. Severity estimated based on incidence and vice versa can be used by technical assistance in the monitoring of onion downy mildew. As the incidence evaluation is more rapid, more accurate and reproducible, according to the obtained data, severity can be estimated based on incidence, facilitating downy mildew quantification by technical assistance.
DOI: 10.6084/m9.figshare.5772627
title: Evaluation of the domestic wastewater treatment efficiency by micronucleus test on Oreochromis niloticus (Cichlidae)

description: ABSTRACT This study aimed at evaluating the efficiency in the domestic wastewater treatment from a city of Sao Paulo State (Brazil), by micronuclei and nuclear abnormalities analysis in erythrocytes of tilapias exposed to raw and treated wastewater. Two samples were collected. Three aquariums of 20 L, with five fish each, were used in the bioassays. In the first, the animals were exposed to the artesian water (negative control); in the second, to the diluted raw wastewater; and in the third, to after wastewater treatment. After the exposure time (72 h), the blood was collected for preparing the smear slides. The results showed genotoxic and mutagenic effects of the raw wastewater in both samples, showing that the process used was efficient to minimize these effects.
DOI: 10.6084/m9.figshare.5772627.v1
title: Evaluation of the domestic wastewater treatment efficiency by micronucleus test on Oreochromis niloticus (Cichlidae)

description: ABSTRACT This study aimed at evaluating the efficiency in the domestic wastewater treatment from a city of Sao Paulo State (Brazil), by micronuclei and nuclear abnormalities analysis in erythrocytes of tilapias exposed to raw and treated wastewater. Two samples were collected. Three aquariums of 20 L, with five fish each, were used in the bioassays. In the first, the animals were exposed to the artesian water (negative control); in the second, to the diluted raw wastewater; and in the third, to after wastewater treatment. After the exposure time (72 h), the blood was collected for preparing the smear slides. The results showed genotoxic and mutagenic effects of the raw wastewater in both samples, showing that the process used was efficient to minimize these effects.
DOI: 10.6084/m9.figshare.5772624
title: Pharmaceuticals and endocrine disrupting compounds in Brazilian waters: occurrence and removal techniques

description: ABSTRACT The human activities have increased the presence of several microcontaminants in water bodies. Of these, more recently, drugs and endocrine disrupters have called the attention of the scientific community due to their increasing exposure. The sources of contamination include cleaning and personal hygiene products, various classes of drugs, natural hormones and their by-products, as well as several substances applied to the production of plastics and resins, present in sewage, landfill leachate and industrial effluents. In this perspective, this paper aims to compile the data of microcontaminants occurrence in Brazilian natural and treated waters, as well as to discuss the efficiency of different water treatment techniques to remove such compounds.
DOI: 10.6084/m9.figshare.5772624.v1
title: Pharmaceuticals and endocrine disrupting compounds in Brazilian waters: occurrence and removal techniques

description: ABSTRACT The human activities have increased the presence of several microcontaminants in water bodies. Of these, more recently, drugs and endocrine disrupters have called the attention of the scientific community due to their increasing exposure. The sources of contamination include cleaning and personal hygiene products, various classes of drugs, natural hormones and their by-products, as well as several substances applied to the production of plastics and resins, present in sewage, landfill leachate and industrial effluents. In this perspective, this paper aims to compile the data of microcontaminants occurrence in Brazilian natural and treated waters, as well as to discuss the efficiency of different water treatment techniques to remove such compounds.
DOI: 10.15156/bio/sh3282242.08fu
title: SH3282242.08FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
DOI: 10.15156/bio/sh3282243.08fu
title: SH3282243.08FU

description: UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.