cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.ns1rn8pv9
title: The angle of attack: Rapping technique predicts skill in hermit crab contests

description: Skill, the ability to perform a challenging behaviour well, has been shown
 to be an important determinant of success in a variety of contexts,
 including human sports, animal courtship and most recently, animal
 contests. Because skilful movement requires precise motor control, skill
 is assumed to be underpinned by traits that determine these abilities.
 However, while these traits determine an individual’s potential to perform
 skilful movements (known as technique), this potential may not translate
 into skilful fighting due to interference from the opponent. Here, we
 investigate the relationship between technique and skill using the
 European hermit crab Pagurus bernhardus. By examining the spatial
 distribution of shell raps, we find that on average, technique exhibited
 during a “training” fight predicts the level of skill displayed in a real
 contest. However, our results also demonstrate substantial
 among-individual variation in the direction of change across the two
 fights, with some individuals exhibiting better technique than skill and
 others showing the opposite pattern. Finally, we find that winners, but
 not losers, progressively adapt their targeting of strikes when faced with
 a fully functional opponent. Our results indicate that skill is a
 combination of innate technique and the ability to adapt to an opponent’s
 behaviour.
DOI: 10.5061/dryad.6hdr7sr28
title: Two-photon imaging of D1 and D2 type neurons in the olfactory tubercle of behaving mice

description: Positive and negative associations acquired through olfactory experience
 are thought to be especially strong and long-lasting. The conserved direct
 olfactory sensory input to the ventral striatal olfactory tubercle (OT)
 and its convergence with dense dopaminergic input to the OT could underlie
 this privileged form of associative memory, but how this process occurs is
 not well understood. We imaged the activity of the two canonical types of
 striatal neurons, expressing D1 or D2 type dopamine receptors, in the OT
 at cellular resolution while mice learned odor-outcome associations
 ranging from aversive to rewarding. D1 and D2 neurons both responded to
 rewarding and aversive odors. D1 neurons in the OT robustly and
 bidirectionally represented odor valence, responding similarly to odors
 predicting similar outcomes regardless of odor identity. This valence
 representation persisted even in the absence of an instrumental response
 to the odors and in the absence of the outcomes, indicating a true
 transformation of odor sensory information by D1 OT neurons. In contrast,
 D2 neuronal representation of the odor-outcome associations was weaker,
 contingent on an instrumental response by the mouse, and D2 neurons were
 more selective for odor identity than valence. Stimulus valence coding in
 the OT was not modality invariant, with separate sets of D1 neurons
 responding to odors and sounds predicting the same outcomes, suggesting
 that integration of multimodal valence information happens downstream of
 the OT. Our results point to distinct representation of identity and
 valence of odor stimuli by D1 and D2 neurons in the OT.
DOI: 10.25349/d95s4j
title: Experience-dependent plasticity modulates ongoing activity in the antennal lobe and enhances odor representations

description: Ongoing neural activity has been observed across several brain regions and
 is thought to reflect the internal state of the brain. Yet, it is
 important to understand how ongoing neural activity interacts with sensory
 experience, and shapes sensory representations. Here, we show that
 projection neurons of the fruit fly antennal lobe exhibit spatiotemporally
 organized ongoing activity. After repeated exposure to odors, we observe a
 gradual and cumulative decrease in the amplitude and number of calcium
 events occurring in the absence of odor stimulation, as well as a
 reorganization of correlations between olfactory glomeruli. Accompanying
 these plastic changes, we find that repeated odor experience reduces
 trial-to-trial variability and enhances the specificity of odor
 representations. Our results reveal an odor experience-dependent
 modulation of ongoing and sensory-evoked activity at peripheral levels of
 the fruit fly olfactory system.
description: The methods for data collection and processing are described in
 the associated manuscript.
DOI: 10.5061/dryad.8gtht76qn
title: Introduced plants induce outbreaks of a native pest and facilitate invasion in the plants’ native range: Evidence from the emerald ash borer

description: 1. Biological invasions are among the most serious threats to native
 forest ecosystems worldwide due to ever-increasing international trade and
 global change. Understanding the invasion processes and ecology of
 invasive pests in both newly invaded and native habitats is necessary to
 effectively manage the risks they pose. 2. The emerald ash borer (EAB),
 Agrilus planipennis, is one of the most devastating invasive forest insect
 pests in North America and has also invaded European Russia and parts of
 Europe. Through synthesizing historical data spanning &gt;100 years
 and contemporary field observations in China, we examined EAB’s
 distribution, occurrence, and outbreak frequency in its native range in
 relation to historical introductions and plantings of non-Asian ash trees
 in China. 3. The frequencies and levels of EAB infestations in China
 gradually increased from 1900 to 2021 after a time-lag of 30-50 years
 following introductions and widespread plantings of non-Asian ash trees
 from North America. Increased frequencies of EAB outbreaks following the
 planting of North American ash trees in China may have increased the risk
 of EAB invading North America and other novel regions. 4. Synthesis. Our
 findings demonstrated that planting susceptible non-native host plants can
 induce outbreaks of a native insect pest in its native range, which in
 turn may enhance risks of invading novel regions via human-assisted
 activities (e.g., international trade). In addition, our findings suggest
 that lag-times of several decades between planting susceptible hosts and
 initial pest outbreaks may pose challenges in predicting the true risk of
 invading novel regions. Consequently, comprehensive risk assessment for
 invasive insect pests should consider the role of non-native plants
 introduced or planted in the pest’s native range.
DOI: 10.5061/dryad.bcc2fqzdc
title: GeCKO_NPC_mito - GFP_FNZ_CT + MITO_1_2

description: Cas9-NPCs cells were generated via lentivirus-mediated transduction of the
 Cas9 gene, followed by selection with 10 µg/ml blasticidin. Cas9-NPCs were
 further transduced with lentivirus particles containing a human sgRNA
 library at a MOI of 0.3 to achieve no greater than 1 sgRNA per cell,
 followed by selection with both 1 ug/ml puromycin and 10 μg/ml blasticidin
 for more than 9 days. After, 5 × 106 cells were collected for determining
 initial sgRNA abundance. Infected cells, expanded to 5 × 107 cells, were
 treated with FNZ for 3 days, followed by FACS sorting of GFP-positive
 cells. Genomic DNA was isolated from all samples, and the sgRNA sequences
 were amplified by PCR and sequenced on a HiSeq 2500.
DOI: 10.5061/dryad.gxd2547nr
title: Global invasion history of the emerging plant pathogen Phytophthora multivora

description: Abstract Background: global trade in living plants and plant material has
 significantly increased the geographic distribution of many plant
 pathogens. As a consequence, several pathogens have been first found and
 described in their introduced range where they may cause severe damage on
 naïve host species. Knowing the center of origin and the pathways of
 spread of a pathogen is of importance for several reasons, including
 identifying natural enemies and reducing further spread. Several
 Phytophthora species are well-known invasive pathogens of natural
 ecosystems, including Phytophthora multivora. Following the description of
 P. multivora from dying native vegetation in Australia in 2009, the
 species was subsequently found to be very common in South Africa where it
 does not cause any remarkable disease. There are now reports of P.
 multivora from many other countries worldwide, but not as a commonly
 encountered species in natural environments. Results: a global collection
 of 335 isolates from North America, Europe, Africa, Australia, the Canary
 Islands, and New Zealand was used to unravel the worldwide invasion
 history of P. multivora, using 10 microsatellite markers for all isolates
 and sequence data from five loci from 94 representative isolates. Our
 population genetic analysis revealed an extremely low heterozygosity,
 significant non-random association of loci and substantial genotypic
 diversity suggesting the spread of P. multivora readily by both asexual
 and sexual propagules. The P. multivora populations in South Africa,
 Australia, and New Zealand show the most complex genetic structure, are
 well established and evolutionary older than those in Europe, North
 America and the Canary Islands. Conclusions: according to conducted
 analyses, the world invasion of P. multivora most likely commenced from
 South Africa, which can be considered the center of origin of the species.
 The pathogen was then introduced to Australia, which acted as bridgehead
 population for Europe and North America. Our study highlighted a complex
 global invasion pattern of P. multivora, including both direct
 introductions from the native population and secondary
 spread/introductions from bridgehead populations.
DOI: 10.5281/zenodo.18384199
title: CWE dataset
DOI: 10.5281/zenodo.18384200
title: CWE dataset
DOI: 10.5061/dryad.3ffbg79k3
title: STR genotypes of 195 black grouse individuals

description: The data file contains the individual genotypes of 195 black grouse
 (Lyrurus tetrix) individuals, genotyped at 9 STR (microsatellite) loci.
 The data is curated into 10 subpopulations as decribed in the accompanied
 publication. The data is uploaded in the commonly used genepop file
 format.
description: Samples were collected non-invasively over the whole study area
 (Styria, Austria) in the years 2013, 2015, 2016. Special measurements to
 guarantee best quality DNA where: collection of faeces only from fresh
 snow (maximum 5 days after snowfall). Invasive samples were collected from
 hunted birds during the hunting season. 250 samples were collected and
 processed. Extraction and amplification procedures of
 DNA are described in: Sittenthaler M, Kunz F, Szymusik A, et
 al (2018) Fine-scale genetic structure in an eastern Alpine black grouse
 <i>Tetrao tetrix</i> metapopulation. J Avian Biol 49:e01681.
 https://doi.org/10.1111/jav.01681.
 The raw fas files from fragment length analysis were
 processed using the PeakScanner 2 software to get the
 genotypes.
description: File according to the genepop file format, as created by GenAlEx
 6.5. File format uses the 2 digit code.
 Missing loci coded as: 0000.
DOI: 10.5061/dryad.qnk98sfj6
title: Extensive introgression at late stages of species formation: Insights from grasshopper hybrid zones

description: The process of species formation is characterised by the accumulation of
 multiple reproductive barriers. The evolution of hybrid male sterility, or
 Haldane’s rule, typically characterises later stages of species formation,
 when total reproductive isolation is strongest. Yet, understanding how
 quickly reproductive barriers evolve and their consequences for
 maintaining genetic boundaries between emerging species remains a
 challenging task because it requires studying taxa that hybridise in
 nature. Here, we address these questions using the meadow grasshopper
 Pseudochorthippus parallelus, where populations that show multiple
 reproductive barriers, including hybrid male sterility, hybridise in two
 natural hybrid zones. Using mitochondrial data, we infer that such
 populations have diverged some 100,000 years ago, at the beginning of the
 last glacial cycle in Europe. Nuclear data shows that contractions at
 multiple glacial refugia, and post-glacial expansions have facilitated
 genetic differentiation between lineages that today interact in hybrid
 zones. We find extensive introgression throughout the sampled species
 range, irrespective of current strength of reproductive isolation.
 Populations exhibiting hybrid male sterility in two hybrid zones show
 repeatable patterns of genomic differentiation, consistent with shared
 genomic constraints affecting ancestral divergence or with the role of
 those regions in reproductive isolation. Together, our results suggest
 that reproductive barriers that characterise late stages of species
 formation can evolve relatively quickly within species, particularly when
 associated with strong demographic changes. Moreover, we show that such
 barriers persist in the face of extensive gene flow, allowing future
 studies to identify associated genomic regions.
DOI: 10.5061/dryad.7wm37pvv4
title: The overlooked complexity of avian brood parasite–host relationships

description: The interactions between avian brood parasites and their hosts have become
 widely recognised as model systems for studying coevolutionary processes.
 These systems have traditionally been viewed as relationships between one
 species of brood parasite and one species of host; however, with most
 brood parasites being known to parasitise multiple species of host and
 hosts often being subject to parasitism by multiple brood parasite
 species, opportunities to examine the ecology and evolution of
 multispecies interactions have generally been overlooked. Here, we compile
 data on all known brood parasite–host relationships and investigate where
 and how multiple species of brood parasites and hosts coexist. Considering
 these relationships as interactions in ecological networks, we find that
 complex brood parasite–host systems (i.e. those that include multiple
 species of brood parasites and hosts) exhibiting highly connected networks
 are found globally, with increased prevalence at lower latitudes in
 tropical and sub-tropical ecosystems. We also examine patterns of past
 research, outline the disparity between global patterns of network
 complexity and past research emphases, and discuss spatial and temporal
 factors that may be associated with these observed patterns. Drawing on
 insights gained from the handful of brood parasitism studies involving
 more complex scenarios and other biological systems that have embraced the
 use of a multispecies framework, we highlight the potential benefits of
 considering brood parasite–host interactions as ecological networks and
 brood parasitism as a model system for studying multispecies interactions.
 Overall, our results provide new insights into the diversity of these
 relationships when considered in a multispecies framework, highlight the
 stark mismatch between past research efforts and global patterns of system
 complexity, and draw attention to the opportunities that the study of more
 complex arrangements offers for examining how species interactions shape
 global patterns of biodiversity.
DOI: 10.5061/dryad.bzkh189b4
title: Differences in local immune cell landscape between Q fever and atherosclerotic abdominal aortic aneurysms identified by multiplex immunohistochemistry

description: Background: Chronic Q fever is a zoonosis caused by the bacterium Coxiella
 burnetii which can manifest as infection of an abdominal aortic aneurysm
 (AAA). Antibiotic therapy often fails, resulting in severe morbidity and
 high mortality. Whereas previous studies have focused on inflammatory
 processes in blood, the aim of this study was to investigate local
 inflammation in aortic tissue. Methods: Multiplex immunohistochemistry was
 used to investigate local inflammation in Q fever AAAs compared to
 atherosclerotic AAAs in aorta tissue specimen. Two six-plex panels were
 used to study both the innate and adaptive immune system. Results: Q fever
 AAAs and atherosclerotic AAAs contained similar numbers of CD68+
 macrophages and CD3+ T cells. However, in Q fever AAAs the number of
 CD68+CD206+ M2 macrophages was increased, while expression of GM-CSF was
 decreased compared to atherosclerotic AAAs. Furthermore, Q fever AAAs
 showed an increase in both the number of CD8+ cytotoxic T cells and
 CD3+CD8-FoxP3+ regulatory T cells. Lastly, Q fever AAAs did not contain
 any well-defined granulomas. Conclusions: These findings demonstrate that
 despite the presence of pro-inflammatory effector cells, persistent local
 infection with C. burnetii is associated with an immune suppressed micro
 environment. Funding: This work was supported by SCAN consortium: European
 Research Area - CardioVascualar Diseases (ERA-CVD) grant [JTC2017-044] and
 TTW-NWO open technology grant [STW-14716].
DOI: 10.5061/dryad.djh9w0w1n
title: Variation in foraging activity influences area-restricted search behaviour by bottlenose dolphins

description: Area-restricted search (ARS) behaviour is commonly used to characterise
 spatio-temporal variation in foraging activity of predators, but evidence
 of the drivers underlying this behaviour in marine systems is sparse.
 Advances in underwater sound recording techniques and automated processing
 of acoustic data now provide opportunities to investigate these questions
 where species use different vocalisations when encountering prey. Here, we
 used passive acoustics to investigate drivers of ARS behaviour in a
 population of dolphins, to determine if residency in key foraging areas
 increased following encounters with prey. Analyses were based on two
 independent proxies of foraging: echolocation buzzes (widely used as
 foraging proxies), and bray calls (vocalizations linked to salmon
 predation attempts). Echolocation buzzes were extracted from echolocation
 data loggers and bray calls from broadband recordings by a convolutional
 neural network (CNN). We found a strong positive relationship between the
 duration of encounters and the frequency of both foraging proxies,
 supporting the theory that bottlenose dolphins engage in ARS behaviour in
 response to higher prey encounter rates. This study provides empirical
 evidence for one driver of ARS behaviour and demonstrates the potential
 for applying passive acoustic monitoring in combination with deep
 learning-based techniques to investigate the behaviour of vocal
 animals. 
description: Echolocation data loggers
 (CPODs; Chelonia Ltd) and broadband acoustic recorders (SoundTrap ST300HF;
 Ocean Instruments, NZ) were deployed in 2018 to characterise both the
 occurrence and the foraging activity of bottlenose dolphins and
 investigate the drivers of the area-restricted search behaviour. We used
 two independent proxies for foraging: 1) echolocation buzzes, identified
 by modelling echolocation inter-click intervals (Pirota et al., 2014); and
 2) bray calls, automatically detected using deep learning techniques,
 building upon the methodology of Bergler et al. (2019).
description: Data consist of 11 files
 and include the datasets and R code required to repeat all the analyses. A
 full description of the files is provided in the Readme.txt
 file: 
 OFB_VarForARS_ClickDetails-2018SutChaCPODS.txt
 OFB_VarForARS_ClickDetails-Buzzes-2018SutChaCPODS.txt
 OFB_ VarForARS_Depl_952_AI_AnnotationResults.csv
 OFB_ VarForARS_Depl_953_AI_AnnotationResults.csv
 OFB_ VarForARS_2018_TideTimes.txt OFB_
 VarForARS_AI_CPOD_Encounter_PropForagingMinutes_Tides.txt
 OFB_ VarForARS_ RCode-DatasetPreparation.R
 OFB_ VarForARS_RCode-StatisticalAnalyses.R
 OFB_ VarForARS_RCode-DataSummaries_Plots.R
 OFB_VarForARS_RCode_StatisticalAnalyses_SupMat3.R
 OFB_ VarForARS_README.txt
DOI: 10.5061/dryad.zpc866t9d
title: Weather driven demography and population dynamics of an endemic perennial plant during a 34-year period

description: 1. Increased anthropogenic influence on the environment has accentuated
 the need to assess how climate and other environmental factors drive vital
 rates and population dynamics of different types of organisms. However, to
 allow distinction between effects of multiple correlated variables, and to
 capture the effects of rare and extreme climatic conditions, studies
 extending over decades are often necessary. 2. In this study we used an
 individual-based dataset collected in three populations of Pulsatilla
 vulgaris subsp. gotlandica during 34 years, to explore the effects of
 variation in precipitation and temperature on vital rates and population
 dynamics. 3. Most of the observed conspicuous variation in flowering among
 years was associated with differences in precipitation and temperature in
 the previous summer and autumn with a higher incidence of flowering
 following summers with high precipitation and low temperatures. In
 contrast, climatic variables had no significant effects on individual
 growth or survival. 4. Although the weather-driven variation in flowering
 had only moderate absolute effects on the population growth rate,
 simulated persistent changes in average precipitation and temperature
 resulted in considerable reductions in population sizes compared with
 current conditions. Analyses carried out with with subsets of data
 consisting of 5 and 10 years yielded results that strongly deviated from
 those based on the full data set. 5. Synthesis: The results of this study
 illustrate the importance of long-term demographic monitoring to identify
 key climatic variables affecting vital rates and driving population
 dynamics in long-lived organisms.
DOI: 10.34740/kaggle/dsv/14647970
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.5061/dryad.3tx95x6hd
title: Ground-foraging ant communities in invaded (by Hovenia dulcis) and non-invaded Atlantic Forest sites, and ant-H. dulcis diaspore interactions

description: Biological invasions are a worldwide threat to biodiversity.
 Invasive species can affect biodiversity in many ways,
 such as by altering habitat properties and structure to which native
 species are dependent. Recipient communities that have
 mutualistic interactions with invasive species, such as seed
 dispersal mutualistic networks, are more vulnerable to invasion. The
 japanese raisin tree, Hovenia dulcis Thunberg
 (Rhamnaceae), is an example of a successful invasive tree in the Brazilian
 Atlantic Forest, where it alters plant communities and leaf-litter inputs.
 Also, Hovenia dulcis invests a massive amount of energy
 in the production of infructescences with fleshy sugary peduncle, and
 several animal species are local consumers and dispersers. We studied the
 relationships between H. dulcis and ground-foraging ant
 communities. Ants are known to be dependent on habitat structure, and have
 been suggested as potential dispersals of H.
 dulcis diaspores. We collected data to evaluate the (i) effects
 of the H. dulcis invasion on ants, and (ii) ant-diaspore
 interactions. We sampled ant communities with pitfall traps in seven
 paired invaded and uninvaded forest sites (n=14) in two close
 times: before (June 2018) and after H.
 dulcis infructescence ripening and falling into the forest floor
 (July 2018). We additionally performed an experiment to understand the
 potential use of H. dulcis diaspores by ant species.