DOI: 10.22002/rec9p-nn519
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-09-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2017-09-29-11_slicer18871.jpg: long filamentous cytoplasmic structures dga2017-09-29-11_slicer18872.jpg: cells division attached dga2017-09-29-11_slicer18873.jpg: more filamentous cytoplasmic structures dga2017-09-29-11_slicer18874.jpg: more filamentous cytoplasmic structures
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-29-11/rawdata/</p>Cb_d5pH4_201709290022.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
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        Cb_d5pH4_201709290022_part61_23.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
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        dga2017-09-29-11_slicer18713.png,  Tubular structure. Visible protofilaments, 880.5 kB  
        
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        dga2017-09-29-11_slicer18714.png,  Tubular structure. Visible protofilaments, 533.7 kB  
        
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        dga2017-09-29-11_slicer18715.png,  Bundle of filaments, 530.2 kB  
        
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