DOI: 10.5061/dryad.vdncjsz4q
title: Data from: Pharyngeal mechanosensory neurons control food swallow in Drosophila melanogaster

description: As the early step of food ingestion, the swallow is under rigorous
 sensorimotor control. Nevertheless, the mechanisms underlying swallow
 control at a molecular and circuitry level remain largely unknown. Here,
 we find that mutation of the mechanotransduction channel
 genes nompC, Tmc, or piezo impairs the regular pumping rhythm of the cibarium during feeding of the fruit fly Drosophila melanogaster. A group of multi-dendritic mechanosensory neurons, which co-express the three channels, wrap the cibarium and are crucial for coordinating the filling and emptying of the cibarium. Inhibition of them causes difficulty in food emptying in the cibarium, while their activation leads to difficulty in cibarium filling. Synaptic and functional connections are detected between the pharyngeal mechanosensory neurons and the motor circuit that controls swallow. This study elucidates the role of mechanosensation in swallow, and provides insights for a better understanding of the neural basis of food swallow.
description: # Dataset for Pharyngeal Mechanosensory Neurons Control Food Swallow in
 Drosophila melanogaster
 [https://doi.org/10.5061/dryad.vdncjsz4q](https://doi.org/10.5061/dryad.vdncjsz4q) ## Description of the data and file structure This dataset includes raw data of confocal microscopy and calcium imaging, which was used in the figures of [https://doi.org/10.7554/eLife.88614](https://doi.org/10.7554/eLife.88614.2) Analysis file was built in GraphPad 8, which is associated with behavior experiment and calcium imaging. Raw data of behavior videos (60fps avi video of *Drosophila* behavior) are 386GB, and we offer access to them in the behavior file description. Questions about this dataset and further requests should be directed to [wei_zhang@mail.tsinghua.edu.cn](mailto:wei_zhang@mail.tsinghua.edu.cn) ### Files and variables #### File: behavior.zip **Description:** The folder consists of behavior analysis file built in GraphPad and URL of behavior videos, which are used in the article. It is about swallowing behavior of flies with md-C neurons manipulated or mechanoreceptor mutated. It includes: figure1B-D, figureS1A-C.pzfx figure1E, figure2, figureS1D.pzfx figure2A, figureS3B-C.pzfx Figure3C, figure4K, figureS4.pzfx TNP-mutant-figureS1-E-H.pzfx URL of behavior videos.txt File naming convention : the corresponding experiment and animal genotype were noted in the file. Any interested researchers could access the behavior videos via URL of BaiduNetdisk, where people could download and watch the videos for free: [https://pan.baidu.com/s/1wUx45mykAUeRCPJiGI0lpQ?pwd=bzjz](https://pan.baidu.com/s/1wUx45mykAUeRCPJiGI0lpQ?pwd=bzjz) #### File: confocal.zip **Description:** The folder consists of raw data for confocal microscopy used in Figure 2, S2, S3 and 4 of the article. It is about fly brain and cibarium neurons participating in controlling swallow. It includes: Tmc-GAL4, nompC-QF&gt;&gt;mCherrry/GFP/RedStinger;  among them, cibairum muscle of fly was dyed with phalloidin in file named tmc(ii)-nompc-flp-out-GFP-cibarium-1 (figure4) Tmc, nompC, piezo, Tmc&piezo, nompC&piezo &gt; GFP; Tmc-GAL4, nompC-QF&gt;&gt;GFP; and labellum of fly was ablated. GRASP imaging between motor neuron 11/12 and md-C neurons. File naming convention: Folder name inside indicates which figure the data was used in. File name inside each second folder indicates the genotype of *Drosophila*, and its body part (brain or cibarium/VCSO, sometimes was abbreviated to B or C/v). Tmc-GAL4,nompC-QF driver was abbreviated to TN. The number in the end of file name indicates the order number of candidate sample in the day's microscopy. Researchers could drag the oif file into ImageJ/Fiji to analyse. #### File: calcium\_imaging.zip **Description:** The folder consists of raw data for calcium imaging used in figure4D-K of the article. It is about calcium imaging in motor neuron 12/11 while activating md-C neurons , and calcium imaging in md-C neurons while fly is swallowing. It includes: Tmc-GAL4&gt;P2X2, MN11/12&gt;GCaMP6s; and control Tmc-GAL4, nompC-QF&gt;&gt;GCaMP6m while swallowing; and control File naming convention:  "MN11" folder includes raw data of Figure4G-I, "MN12" folder includes raw data of Figure4D-F, they are about calcium imaging in motor neuron 12/11 while activating md-C neurons, and folder name inside indicates the genotype and added stimuli (water or ATP). "TN-gcamp6" folder includes raw data of Figure4J-K, where fly's genotype is Tmc-GAL4,nompC-QF (abbreviated to TN)&gt;&gt;GCaMP6m. Researchers could drag the tif file into ImageJ/Fiji to analyse. ## Code/software GraphPad ImageJ/Fiji Excel ## Access information Other publicly accessible locations of the data: * N/A Data was derived from the following sources: * N/A