cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.22828211
title: Chromosome diversity in Buthidae and Chactidae scorpions from Brazilian fauna: Diploid number and distribution of repetitive DNA sequences

description: Abstract In this work, we analyzed cytogenetically eight Chactidae and Buthidae, including the localization of repetitive DNA sequences. The chactids possess monocentric chromosomes and the highest diploid numbers (2n=50 in Brotheas amazonicus, 2n=36 in Chactopsis amazonica, 2n=30 in Neochactas sp.) when compared with buthids (2n=10 in Tityus bahiensis, 2n=14 in Tityus apiacas and Tityus metuendus, 2n=18 in Tityus aba, 2n=26 in Ischnotelson peruassu). The localization of rDNA genes and (TTAGG)n sequences exhibited a conserved pattern of two terminal/subterminal ribosomal cistrons and terminal telomere signals. However, the comparison between the data of C-banding, DAPI after FISH and Cot-DNA fraction indicated a variable quantity and distribution of these regions, as follow: (i) positive heterochromatin and Cot-DNA signals (B. amazonicus and I. peruassu), (ii) small blocks of heterochromatin with large Cot-DNA signals (T. metuendus), (iii) positive heterochromatic regions and absence of Cot-DNA signals (T. aba and T. apiacas), and (iv) negative heterochromatin and Cot-DNA signals (T. bahiensis). Therefore, our results revealed that there still is not a clear relation between quantity of heterochromatin and presence of monocentric or holocentric chromosomes and occurrence of chromosomal rearrangements, indicating that repetitive regions in scorpions must be analyzed using different cytogenetic approaches.
DOI: 10.6084/m9.figshare.22828211.v1
title: Chromosome diversity in Buthidae and Chactidae scorpions from Brazilian fauna: Diploid number and distribution of repetitive DNA sequences

description: Abstract In this work, we analyzed cytogenetically eight Chactidae and Buthidae, including the localization of repetitive DNA sequences. The chactids possess monocentric chromosomes and the highest diploid numbers (2n=50 in Brotheas amazonicus, 2n=36 in Chactopsis amazonica, 2n=30 in Neochactas sp.) when compared with buthids (2n=10 in Tityus bahiensis, 2n=14 in Tityus apiacas and Tityus metuendus, 2n=18 in Tityus aba, 2n=26 in Ischnotelson peruassu). The localization of rDNA genes and (TTAGG)n sequences exhibited a conserved pattern of two terminal/subterminal ribosomal cistrons and terminal telomere signals. However, the comparison between the data of C-banding, DAPI after FISH and Cot-DNA fraction indicated a variable quantity and distribution of these regions, as follow: (i) positive heterochromatin and Cot-DNA signals (B. amazonicus and I. peruassu), (ii) small blocks of heterochromatin with large Cot-DNA signals (T. metuendus), (iii) positive heterochromatic regions and absence of Cot-DNA signals (T. aba and T. apiacas), and (iv) negative heterochromatin and Cot-DNA signals (T. bahiensis). Therefore, our results revealed that there still is not a clear relation between quantity of heterochromatin and presence of monocentric or holocentric chromosomes and occurrence of chromosomal rearrangements, indicating that repetitive regions in scorpions must be analyzed using different cytogenetic approaches.
DOI: 10.6084/m9.figshare.22828214
title: Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis

description: ABSTRACT: The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis and assessed if the histopathological examination and polymerase chain reaction (PCR) were effective for studies like this. Trachea, liver, and lung were collected from 30 chickens with suspected airsacculitis that has been condemned in the inspection line. The samples were analyzed by PCR to simultaneously identify two virulence genes (iss and tsh genes) and for histopathological testing. PCR efficiently genotypically characterize the E. coli isolates, where the virulence genes iss and tsh were found in three birds simultaneously. The histopathological examination detected a predominance of heterophils and mononuclear cells in the trachea (100%), lung (90%), and liver (13.3%). The liver was the organ where practically no alteration was diagnosed. The results of multiplex PCR for the tsh and iss virulence genes indicate the great potential of the approach in the characterization of E. coli isolates. Unspecific identification did not occur, thus making it necessary to use technologies for the identification and prevention of this agent in aviaries and poultry abattoirs.
DOI: 10.6084/m9.figshare.22828214.v1
title: Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis

description: ABSTRACT: The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis and assessed if the histopathological examination and polymerase chain reaction (PCR) were effective for studies like this. Trachea, liver, and lung were collected from 30 chickens with suspected airsacculitis that has been condemned in the inspection line. The samples were analyzed by PCR to simultaneously identify two virulence genes (iss and tsh genes) and for histopathological testing. PCR efficiently genotypically characterize the E. coli isolates, where the virulence genes iss and tsh were found in three birds simultaneously. The histopathological examination detected a predominance of heterophils and mononuclear cells in the trachea (100%), lung (90%), and liver (13.3%). The liver was the organ where practically no alteration was diagnosed. The results of multiplex PCR for the tsh and iss virulence genes indicate the great potential of the approach in the characterization of E. coli isolates. Unspecific identification did not occur, thus making it necessary to use technologies for the identification and prevention of this agent in aviaries and poultry abattoirs.
DOI: 10.6084/m9.figshare.22828216
title: Amalgam x Composite Resin: supplies and restorative procedures more performed among Oral Health Teams in Brazil

description: Abstract This study aimed to compare the availability of supplies and amalgam and composite resin restorations among Oral Health Teams (OHT) in Brazilian regions. Secondary data were extracted from Modules I and II of the 1st (2012) and V and VI of the 2nd (2014) and 3rd cycle (2017) of the external evaluation of the National Program for Access and Quality in Primary Care2. The proportions between regions and cycles were compared using the Chi-square test with the z-test adjusted by the Bonferroni method (p&lt;0.05). Among 2012, 2014, and 2017 there was a significant reduction in the proportion of OHT that performed amalgam restorations (87.5%, 89.2%, and 80.2%; p&lt;0.001) and an increase in resin composite (92.5 %, 97.7%, and 99.0%; p&lt;0.001), with the same trend in Brazilian regions (p&lt;0.001). Amalgamator availability decreased among 2012 (99.0%), 2014 (98.4%) and 2017 (85.6%) (p&lt;0.001). Amalgam availability was lower in 2017 (80.1%), compared to 2012 (87.5%) and 2014 (97.5%) (p&lt;0.001). The availability of light curing decreased between 2012 (99.0%), 2014 (98.4%) and 2017 (85.6%) (p&lt;0.001), being less available in the North (95.7%) (p&lt;0.001). The light-curing resin increased between 2012 (94.1%), 2014 (96.6%) and 2017 (97.0%) (p&lt;0.001), with no increase only in the North (p=0.134). While there was a reduction in supplies and amalgam restoration, there was an increase in supplies and resin composite restorations in the period evaluated in all Brazilian regions. However, regional disparities are still evident, with fewer supplies of restorative services in the North region.
DOI: 10.6084/m9.figshare.22828216.v1
title: Amalgam x Composite Resin: supplies and restorative procedures more performed among Oral Health Teams in Brazil

description: Abstract This study aimed to compare the availability of supplies and amalgam and composite resin restorations among Oral Health Teams (OHT) in Brazilian regions. Secondary data were extracted from Modules I and II of the 1st (2012) and V and VI of the 2nd (2014) and 3rd cycle (2017) of the external evaluation of the National Program for Access and Quality in Primary Care2. The proportions between regions and cycles were compared using the Chi-square test with the z-test adjusted by the Bonferroni method (p&lt;0.05). Among 2012, 2014, and 2017 there was a significant reduction in the proportion of OHT that performed amalgam restorations (87.5%, 89.2%, and 80.2%; p&lt;0.001) and an increase in resin composite (92.5 %, 97.7%, and 99.0%; p&lt;0.001), with the same trend in Brazilian regions (p&lt;0.001). Amalgamator availability decreased among 2012 (99.0%), 2014 (98.4%) and 2017 (85.6%) (p&lt;0.001). Amalgam availability was lower in 2017 (80.1%), compared to 2012 (87.5%) and 2014 (97.5%) (p&lt;0.001). The availability of light curing decreased between 2012 (99.0%), 2014 (98.4%) and 2017 (85.6%) (p&lt;0.001), being less available in the North (95.7%) (p&lt;0.001). The light-curing resin increased between 2012 (94.1%), 2014 (96.6%) and 2017 (97.0%) (p&lt;0.001), with no increase only in the North (p=0.134). While there was a reduction in supplies and amalgam restoration, there was an increase in supplies and resin composite restorations in the period evaluated in all Brazilian regions. However, regional disparities are still evident, with fewer supplies of restorative services in the North region.
DOI: 10.6084/m9.figshare.22828217
title: The Covid-19 pandemic: a traumatic event for health and biological science students?

description: Abstract: Introduction: Health Sciences university students were especially affected by the Sars-CoV-2 virus pandemic. The anxieties intrinsically related to Covid-19 were added to the impacts related to the changes that have taken place in academic institutions, particularly the change to online teaching, a methodology that can generate discomfort for students, in addition to several obstacles related to teaching and learning, which can have important traumatic effects on the mental health of this population. Objective: To assess the mental health burden caused by the COVID-19 pandemic, as a traumatic event capable of triggering psychiatric disorders, such as Post-Traumatic Stress Disorder (PTSD), in university students. Methods: This is a cross-sectional and quantitative study, of analytical-descriptive nature, carried out by filling out an anonymous digital form, initiated after prior approval by the Research Ethics Committee. The subjects were students of Biological and Health Sciences at a State University located in the southeastern region of Brazil. The sample consisted of 618 students. Results: 618 forms were answered by students attending the courses of Physical Education (28.8%), Medicine (25.4%), Dentistry (18.1%), Biological Sciences (15.2%) and Nursing (12.5%). The presence of post-traumatic stress symptoms assessed by the Impact of Event Scale - Revised (IES-R) obtained a prevalence of 32.7% (n = 202) among university students from Biological and Health Sciences courses. Conclusions: There was a significant impact on the mental health of university students, with the presence of depressive symptoms, anxiety and stress above those found in the scientific literature and, consequently, a high prevalence of PTSD.
DOI: 10.6084/m9.figshare.22828217.v1
title: The Covid-19 pandemic: a traumatic event for health and biological science students?

description: Abstract: Introduction: Health Sciences university students were especially affected by the Sars-CoV-2 virus pandemic. The anxieties intrinsically related to Covid-19 were added to the impacts related to the changes that have taken place in academic institutions, particularly the change to online teaching, a methodology that can generate discomfort for students, in addition to several obstacles related to teaching and learning, which can have important traumatic effects on the mental health of this population. Objective: To assess the mental health burden caused by the COVID-19 pandemic, as a traumatic event capable of triggering psychiatric disorders, such as Post-Traumatic Stress Disorder (PTSD), in university students. Methods: This is a cross-sectional and quantitative study, of analytical-descriptive nature, carried out by filling out an anonymous digital form, initiated after prior approval by the Research Ethics Committee. The subjects were students of Biological and Health Sciences at a State University located in the southeastern region of Brazil. The sample consisted of 618 students. Results: 618 forms were answered by students attending the courses of Physical Education (28.8%), Medicine (25.4%), Dentistry (18.1%), Biological Sciences (15.2%) and Nursing (12.5%). The presence of post-traumatic stress symptoms assessed by the Impact of Event Scale - Revised (IES-R) obtained a prevalence of 32.7% (n = 202) among university students from Biological and Health Sciences courses. Conclusions: There was a significant impact on the mental health of university students, with the presence of depressive symptoms, anxiety and stress above those found in the scientific literature and, consequently, a high prevalence of PTSD.
DOI: 10.6084/m9.figshare.22828219
title: Sampling intensity and size to evaluate harvest losses in soybean crops

description: ABSTRACT Proper sampling of grain losses during harvesting operations, with reliable and efficient sizing of sample, is necessary for an efficient adjustment of the harvester to avoid harvest losses. Thus, the objective of this study was to estimate sampling intensity and sample size for harvest loss evaluations in soybean crops. Sampling was carried out in five locations with soybean crops, evaluating three different harvesters. Harvest losses were measured using square wooden frames (50 × 50 cm), which were arranged on the ground longitudinally across the harvester header width after its passage; this process was repeated 25 times at each location. The greatest harvester header width in Location 1 enabled to simulate different sample sizes for this location (50 × 100, 50 × 150, 50 × 200, and 50 × 250 cm). Only one sample size (50 × 100 cm) was used for the simulations in the other locations. Sampling only half the harvester header width is recommended to estimate harvest losses when using 62-foot harvester header, whereas 12.5- and 17-foot harvester headers requires sampling across the entire harvester header width, with a semi-amplitude of the confidence interval of 20% of the mean for all harvesters.
DOI: 10.6084/m9.figshare.22828219.v1
title: Sampling intensity and size to evaluate harvest losses in soybean crops

description: ABSTRACT Proper sampling of grain losses during harvesting operations, with reliable and efficient sizing of sample, is necessary for an efficient adjustment of the harvester to avoid harvest losses. Thus, the objective of this study was to estimate sampling intensity and sample size for harvest loss evaluations in soybean crops. Sampling was carried out in five locations with soybean crops, evaluating three different harvesters. Harvest losses were measured using square wooden frames (50 × 50 cm), which were arranged on the ground longitudinally across the harvester header width after its passage; this process was repeated 25 times at each location. The greatest harvester header width in Location 1 enabled to simulate different sample sizes for this location (50 × 100, 50 × 150, 50 × 200, and 50 × 250 cm). Only one sample size (50 × 100 cm) was used for the simulations in the other locations. Sampling only half the harvester header width is recommended to estimate harvest losses when using 62-foot harvester header, whereas 12.5- and 17-foot harvester headers requires sampling across the entire harvester header width, with a semi-amplitude of the confidence interval of 20% of the mean for all harvesters.
DOI: 10.6084/m9.figshare.22828222
title: Level of ethical-legal knowledge of medicine graduants: cross-sectional study in the year of 2020

description: Abstract: Introduction: Complaints about medical malpractice are frequent. Therefore, investigating how the medical student formation is made concerning the ethical and legal aspects, can contribute to its improvement. Objective: To evaluate and compare the level of knowledge of medical students from a university about ethical and legal aspects of the profession, based on their formation in the disciplines of the related axis (Medical Ethics and Bioethics; Legal Medicine and Deontology/Medical Forensics and Good Practice). Method: Cross-sectional study carried out through the application of an online questionnaire (August-November/2020) based on the Code of Medical Ethics (CME) and the Code of Ethics for Medical Student- 10 sociodemographic and 12 objective questions (Topics: “Professional Confidentiality”, “Medical student’s Interpersonal Relations”, “Medical Publicity”, “Relations with Patients and Families”, “Human Rights”, “Fundamental Principles”, “Medical Activity” and “Telemedicine”). A total of 116 students were evaluated at the start, middle and end of the medical course. The Kruskal-Wallis and Dunn tests were used for comparisons. The Chi-Square and Fisher’s exact tests were used for the association of categorical variables. Results: The performance increased throughout the course, with statistical significance between the average number of correct answers in the 1st, 6th and 10th/12th periods: 5.1; 6.7 and 7.8, (p-value &lt;0.001). The analysis by thematic blocks, on the other hand, did not show a statistical association of correct answers with progress in the medical course (p-value &gt;0.05) for broad-reaching topics and development of competences from the basic cycle, such as “Medical Publicity”, “Relations with Patients and Families”, “Telemedicine” and “Medical Student’s Interpersonal Relations” - with emphasis on the student’s limitations in providing care. As for topics that required competences inherent to the disciplines of the ethical-legal axis, this association was identified (p-value &lt;0.05), as follows: “Professional Confidentiality”, “Human Rights”, “Fundamental Principles”, “Medical Activity” and “Medical Student’s Interpersonal Relations” - specifically on the use of electronics. Finally, the highest percentage of correct answers (95.7%) is associated with “Medical Student’s Interpersonal Relations”, with an emphasis on the student’s limitations. While the lowest rates were found in “Professional Confidentiality” (18.1%), “Medical Activity” (33.6%) and “Human Rights” (25%)”. Conclusion: There is an association between the medical formation proposed by the ethical-legal axis, and the effective increase of the medical student’s level of knowledge concerning the ethical-legal aspects of the profession.
DOI: 10.6084/m9.figshare.22828222.v1
title: Level of ethical-legal knowledge of medicine graduants: cross-sectional study in the year of 2020

description: Abstract: Introduction: Complaints about medical malpractice are frequent. Therefore, investigating how the medical student formation is made concerning the ethical and legal aspects, can contribute to its improvement. Objective: To evaluate and compare the level of knowledge of medical students from a university about ethical and legal aspects of the profession, based on their formation in the disciplines of the related axis (Medical Ethics and Bioethics; Legal Medicine and Deontology/Medical Forensics and Good Practice). Method: Cross-sectional study carried out through the application of an online questionnaire (August-November/2020) based on the Code of Medical Ethics (CME) and the Code of Ethics for Medical Student- 10 sociodemographic and 12 objective questions (Topics: “Professional Confidentiality”, “Medical student’s Interpersonal Relations”, “Medical Publicity”, “Relations with Patients and Families”, “Human Rights”, “Fundamental Principles”, “Medical Activity” and “Telemedicine”). A total of 116 students were evaluated at the start, middle and end of the medical course. The Kruskal-Wallis and Dunn tests were used for comparisons. The Chi-Square and Fisher’s exact tests were used for the association of categorical variables. Results: The performance increased throughout the course, with statistical significance between the average number of correct answers in the 1st, 6th and 10th/12th periods: 5.1; 6.7 and 7.8, (p-value &lt;0.001). The analysis by thematic blocks, on the other hand, did not show a statistical association of correct answers with progress in the medical course (p-value &gt;0.05) for broad-reaching topics and development of competences from the basic cycle, such as “Medical Publicity”, “Relations with Patients and Families”, “Telemedicine” and “Medical Student’s Interpersonal Relations” - with emphasis on the student’s limitations in providing care. As for topics that required competences inherent to the disciplines of the ethical-legal axis, this association was identified (p-value &lt;0.05), as follows: “Professional Confidentiality”, “Human Rights”, “Fundamental Principles”, “Medical Activity” and “Medical Student’s Interpersonal Relations” - specifically on the use of electronics. Finally, the highest percentage of correct answers (95.7%) is associated with “Medical Student’s Interpersonal Relations”, with an emphasis on the student’s limitations. While the lowest rates were found in “Professional Confidentiality” (18.1%), “Medical Activity” (33.6%) and “Human Rights” (25%)”. Conclusion: There is an association between the medical formation proposed by the ethical-legal axis, and the effective increase of the medical student’s level of knowledge concerning the ethical-legal aspects of the profession.
DOI: 10.6084/m9.figshare.22828223
title: Cytotoxicity and genotoxicity of Bio-C Repair, Endosequence BC Root Repair, MTA Angelus and MTA Repair HP

description: Abstract The aim was to evaluate in vitro cytotoxicity and genotoxicity of Bio-C Repair (BCR), compared to Endosequence BC Root Repair (ERRM), MTA Angelus (MTA-Ang), and MTA Repair HP (MTA-HP). MC3T3 osteoblastic cells were exposed to extracts of the repairing bioceramic cements. After 1, 3, and 7 days, cytotoxicity and genotoxicity were evaluated by MTT and Micronucleus tests, respectively. Cells not exposed to biomaterials were used as a negative control. Data were compared using ANOVA two-way, followed by the Tukey Test (α=5%). MTA-Ang and MTA-HP showed no difference in relation to control regarding cytotoxicity in any experimental times. BCR and ERRM reduced cell viability after 3 and 7 days (p&lt;0.05); however, the reduction caused by BCR was less than that caused by ERRM. Considering the micronucleus formation, all biomaterials caused an increase after 3 and 7 days (p&lt;0.05), being greater for the BCR and ERRM groups. It can be concluded that BCR is non-cytotoxic in osteoblastic cells, as well as MTA-Ang e MTA Repair HP. BCR and ERRM showed greater genotoxicity than others tested biomaterials.