DOI: 10.5061/dryad.76hdr7t12
title: Effects of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury model (Sprague Dawley rats)

description: Background: Spinal cord injury (SCI) is a destructive neurological and
 pathological state that causes major motor, sensory and autonomic
 dysfunctions. It’s final neurological outcome determined from both primary
 and secondary injury process. Neuroinflammation is a key component of the
 secondary injury mechanisms with local and systemic consequences. A
 neuroprotective compound, ACTH4-10Pro8-Gly9-Pro10 also known as Semax has
 shown neuroprotective and anti-inflammatory properties.
 ACTH4-10Pro8-Gly9-Pro10 also has actively used in the treatment of brain
 ischemia without serious complication reported. Here we analyzed the
 effects of ACTH4-10Pro8-Gly9-Pro10 in regulating inflammatory cascade in
 SCI by looking at the expression of anti-inflammatory cytokine IL-4,
 IL-10, IL-13 in acute compression SCI. Method: We do laminectomy in
 Sprague Dawley rats at the second thoracic vertebrae. After laminectomy we
 expose the myelum and create mild SCI model with 20gr and severe SCI with
 35gr aneurysm clips. ACTH4-10Pro8-Gly9-Pro10 was administered intranasally
 to the treatment group and 0,9% NaCl to the control group (placebo). Both
 group was remain alive and terminated at 3 and 6 hour. The preparations
 tissue sample were fixed in formalin and examined for
 immunohistochemistry. Quantitative measurement of anti-inflammatory
 cytokine (IL-4, IL-10, IL-13) was done in posterior horn with associated
 anti-monoclonal antibodies.   Result: Rats with mild SCI that
 were given ACTH4-10Pro8-Gly9-Pro10 shown greater expression of IL-4, IL-10
 and IL-13 at three hour post compression but only IL-10 and IL-13 elevated
 significantly at six hour. Rats with severe compression in
 ACTH4-10Pro8-Gly9-Pro10 group shown greater expression of IL-10, IL-13 at
 three hour and IL-4, IL-10 at six hour compared with the placebo group.
   Conclusion: Administration of ACTH4-10Pro8-Gly9-Pro10
 intranasal can increase anti inflammatory cytokine expression at Sprague
 Dawley rat model with mild and severe SCI. Expression of anti inflammatory
 cytokine was greater in mild compression and early hour (3 hour). Further
 research needs to be done to determine optimal dose and the actual
 clinical outcome in vivo.
description: We
 do laminectomy in Sprague Dawley rats at the second thoracic vertebrae.
 After laminectomy we expose the myelum and create mild SCI model with 20gr
 and severe SCI with 35gr aneurysm clips. ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10 </sup>was administered intranasally to the treatment group and 0,9% NaCl to the control group (placebo). Both group was remain alive and terminated at 3 and 6 hour. The preparations tissue sample were fixed in formalin and examined for immunohistochemistry. Quantitative measurement of anti-inflammatory cytokine (IL-4, IL-10, IL-13) was done in posterior horn with associated anti-monoclonal antibodies.
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