cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7075502
title: Bacterial cellulose to reinforce urethrovesical anastomosis. A translational study

description: Abstract Purpose: To evaluate the efficacy of the cellulosic exopolysaccharide membrane (CEM) as a urethral reinforcement for urethrovesical anastomosis. Methods: Twenty eight rabbits were submitted to urethrovesical anastomosis with or without CEM reinforcement. The animals were divided into 4 groups: C7, CEM7, C14 and CEM14: (C= only anastomosis or CEM = anastomosis + CEM), evaluated after 7 weeks, and 14 weeks. The biointegration and biocompatibility of CEM were evaluated according to stenosis, fistula, urethral wall thickness, urethral epithelium, rate of inflammation and vascularization. Results: Between the two experimental groups, the difference in the number of stenosis or urinary fistula was not statistically significant. The morphometric analysis revealed preservation of urethral lumen, well adhered CEM without extrusion, a controlled inflammatory process and implant vascularization. The urothelium height remained constant over time after CEM reinforcement and the membrane wall was thicker, statistically, after 14 weeks. Conclusion: The absence of extrusion, stenosis or urinary fistula after 14 weeks of urethrovesical anastomosis demonstrates cellulosic exopolysaccharide membrane biocompatibility and biointegration with tendency to a thicker wall.
DOI: 10.6084/m9.figshare.7075508
title: Comparative mitogenomic analyses of Amazona parrots and Psittaciformes

description: Abstract Amazon parrots are long-lived birds with highly developed cognitive skills, including vocal learning. Several parrot mitogenomes have been sequenced, but important aspects of their organization and evolution are not fully understood or have limited experimental support. The main aim of the present study was to describe the mitogenome of the blue-fronted Amazon, Amazona aestiva, and compare it to other mitogenomes from the genus Amazona and the order Psittaciformes. We observed that mitogenomes are highly conserved among Amazon parrots, and a detailed analysis of their duplicated control regions revealed conserved blocks. Population level analyses indicated that the specimen analyzed here seems to be close to A. aestiva individuals from Bahia state. Evolutionary relationships of 41 Psittaciformes species and three outgroups were inferred by BEAST. All relationships were retrieved with high support.
DOI: 10.26165/juelich-data/tvwuup/7cvtss
title: 045.png

description: :unav
DOI: 10.6084/m9.figshare.7075508.v1
title: Comparative mitogenomic analyses of Amazona parrots and Psittaciformes

description: Abstract Amazon parrots are long-lived birds with highly developed cognitive skills, including vocal learning. Several parrot mitogenomes have been sequenced, but important aspects of their organization and evolution are not fully understood or have limited experimental support. The main aim of the present study was to describe the mitogenome of the blue-fronted Amazon, Amazona aestiva, and compare it to other mitogenomes from the genus Amazona and the order Psittaciformes. We observed that mitogenomes are highly conserved among Amazon parrots, and a detailed analysis of their duplicated control regions revealed conserved blocks. Population level analyses indicated that the specimen analyzed here seems to be close to A. aestiva individuals from Bahia state. Evolutionary relationships of 41 Psittaciformes species and three outgroups were inferred by BEAST. All relationships were retrieved with high support.
DOI: 10.26165/juelich-data/tvwuup/tluy0o
title: 696.png

description: :unav
DOI: 10.6084/m9.figshare.7075496
title: Thickness and immunohistochemistry of LASIK flaps created by different femtosecond lasers in eye-bank corneas

description: ABSTRACT Purpose: The aim of this study was to compare the corneal cell viability and thickness of LASIK flaps created by 3 femtosecond lasers in eye-bank human corneas. Methods: Forty-five eye-bank human sclerocorneal buttons (15 corneas in each group) were examined after the creation of 120 mm-thick laser-assisted keratomileusis (LASIK) flaps with 150kHz iFS IntraLase™ (IL), Z6 Femto LDV™ (LDV), or 200kHz Wavelight™ FS200 (FS200). The thickness of the flaps was measured using anterior segment optical coherence tomography (AS-OCT; Visante™). Cell viability was blindly evaluated with immunohistochemistry for keratocyte apoptosis using anti-caspase 3 antibodies. Results: The standard deviation from the intended flap thickness was less than 10 mm in all the groups. There was a statistically significant difference in corneas treated with LDV and IL with regard to the flap thickness horizontally at +3.00 mm (p=0.0124), -0.5 mm (p=0.0082), and -1.00 mm (p=0.0425) from the corneal vertex and +0.5 mm from the flap edge (p=0.0240), and those treated with LDV and FS200 with regard to the flap thickness horizontally at -0.5 mm from the corneal vertex (p=0.0082). The mean keratocyte apoptosis numbers were 13.09 ± 1.10, 15.59 ± 3.28, and 17.72 ± 1.49 in corneas treated with IL, FS200, and LDV, respectively (p&lt;0.001). Conclusion: All 3 assessed femtosecond lasers provided predictable LASIK flap thickness. The mean stromal keratocyte apoptosis number was low in all groups.
DOI: 10.6084/m9.figshare.7075499
title: The use of polyaluminium chloride as a restoration measure to improve water quality in tropical shallow lakes

description: Abstract Aim The aim of this study was to evaluate the performance of the coagulant Polyaluminium chloride (PAC) in water quality improvement of six eutrophic shallow lakes in Brazilian semiarid region. Methods We evaluated the effect of PAC in turbidity, humic substances (UV254), total phosphorus and chlorophyll-a concentration through laboratory jar tests. Results The results showed that PAC had a good performance in reducing total phosphorus concentrations and turbidity, with a reduced efficiency in removing chlorophyll-a and humic substances by sedimentation of flocks formed. Conclusions Addition of PAC is a potential tool for water quality improvement of eutrophic shallow lakes in Brazilian semiarid region but its efficiency depends on the pH and particulate and dissolved organic matter concentration in the lake or reservoir water.
DOI: 10.6084/m9.figshare.7075496.v1
title: Thickness and immunohistochemistry of LASIK flaps created by different femtosecond lasers in eye-bank corneas

description: ABSTRACT Purpose: The aim of this study was to compare the corneal cell viability and thickness of LASIK flaps created by 3 femtosecond lasers in eye-bank human corneas. Methods: Forty-five eye-bank human sclerocorneal buttons (15 corneas in each group) were examined after the creation of 120 mm-thick laser-assisted keratomileusis (LASIK) flaps with 150kHz iFS IntraLase™ (IL), Z6 Femto LDV™ (LDV), or 200kHz Wavelight™ FS200 (FS200). The thickness of the flaps was measured using anterior segment optical coherence tomography (AS-OCT; Visante™). Cell viability was blindly evaluated with immunohistochemistry for keratocyte apoptosis using anti-caspase 3 antibodies. Results: The standard deviation from the intended flap thickness was less than 10 mm in all the groups. There was a statistically significant difference in corneas treated with LDV and IL with regard to the flap thickness horizontally at +3.00 mm (p=0.0124), -0.5 mm (p=0.0082), and -1.00 mm (p=0.0425) from the corneal vertex and +0.5 mm from the flap edge (p=0.0240), and those treated with LDV and FS200 with regard to the flap thickness horizontally at -0.5 mm from the corneal vertex (p=0.0082). The mean keratocyte apoptosis numbers were 13.09 ± 1.10, 15.59 ± 3.28, and 17.72 ± 1.49 in corneas treated with IL, FS200, and LDV, respectively (p&lt;0.001). Conclusion: All 3 assessed femtosecond lasers provided predictable LASIK flap thickness. The mean stromal keratocyte apoptosis number was low in all groups.
DOI: 10.26165/juelich-data/tvwuup/sfnrw3
title: 641.png

description: :unav
DOI: 10.6084/m9.figshare.7075493
title: Does one size fit all? An analysis of portfolio allocation in the Brazilian multiparty presidential system

description: Presidents face a dilemma of whom to appoint for cabinet positions. They need to secure legislative support for their government and at the same time achieve their goals in terms of public policy. This work analyzes the portfolio allocation of Brazilian presidents in Brazil’s multiparty system. This study tests some hypotheses using multinomial logistic regression to identify appointment strategies adopted by the presidents in four different governmental sectors from 1990 to 2016. To do so, first, we create an index of ministerial politicization (IMP) and aggregate the ministries in these four sections, employing cluster analysis. The results show that appointments to the core positions in government tend to be less politicized. Moreover, the president’s personal nominations were greater in the presidential units that carry out the political coordination and typical functions of the State.
DOI: 10.6084/m9.figshare.7075499.v1
title: The use of polyaluminium chloride as a restoration measure to improve water quality in tropical shallow lakes

description: Abstract Aim The aim of this study was to evaluate the performance of the coagulant Polyaluminium chloride (PAC) in water quality improvement of six eutrophic shallow lakes in Brazilian semiarid region. Methods We evaluated the effect of PAC in turbidity, humic substances (UV254), total phosphorus and chlorophyll-a concentration through laboratory jar tests. Results The results showed that PAC had a good performance in reducing total phosphorus concentrations and turbidity, with a reduced efficiency in removing chlorophyll-a and humic substances by sedimentation of flocks formed. Conclusions Addition of PAC is a potential tool for water quality improvement of eutrophic shallow lakes in Brazilian semiarid region but its efficiency depends on the pH and particulate and dissolved organic matter concentration in the lake or reservoir water.
DOI: 10.6084/m9.figshare.7075490
title: Microbiological analysis of contact lens cases: impact of the hospital environment

description: ABSTRACT Purpose: The aim of this study was to analyze the bacterial and fungal microbiota found in contact lens cases among two groups of patients to correlate the data on the habits of contact lens users and to evaluate whether there is a difference in the culture results between users of ridged and nonridged contact lens cases. Methods: Two groups (35 patients per group) were included, consisting of hospital workers and those who had not visited a hospital in the past 30 days, and a questionnaire regarding epidemiological data and habits related to contact lens and lens case use was administered. In addition, 140 samples collected from the right and left compartments of each lens case by swabbing the bottom of the wells were tested using bacterioscopy as well as fungal and bacterial cultures via computerized identification of the species. Results: No fungal growth was identified in any of the 70 contact lens cases; however, bacteria were found in 39 cases, and there was no statistical difference between the groups. Most bacteria (&gt;85%) were gram-negative bacilli. Contamination inone compartment of the contact lens case elevated the risk of contamination of the other side (&gt;80%). Moreover, contamination was statistically higher in the ridged cases than in nonridged cases (p=0.0149). Conclusion: The types of bacteria contaminating the cases are generally not seen in eye diseases associated with contact lens use, suggesting that other decisive variables are involved in eye infection from a contaminated lens or case. Fungal contamination of contact lens cases appears to be an exception. Ridged cases are commonly used by contact lens wearers and present a potential risk to eye health. In addition, the results of bacterial tests between hospital workers and those who did not visit a hospital were not significantly different.
DOI: 10.6084/m9.figshare.7075493.v1
title: Does one size fit all? An analysis of portfolio allocation in the Brazilian multiparty presidential system

description: Presidents face a dilemma of whom to appoint for cabinet positions. They need to secure legislative support for their government and at the same time achieve their goals in terms of public policy. This work analyzes the portfolio allocation of Brazilian presidents in Brazil’s multiparty system. This study tests some hypotheses using multinomial logistic regression to identify appointment strategies adopted by the presidents in four different governmental sectors from 1990 to 2016. To do so, first, we create an index of ministerial politicization (IMP) and aggregate the ministries in these four sections, employing cluster analysis. The results show that appointments to the core positions in government tend to be less politicized. Moreover, the president’s personal nominations were greater in the presidential units that carry out the political coordination and typical functions of the State.
DOI: 10.26165/juelich-data/tvwuup/9bgjty
title: 551.png

description: :unav
DOI: 10.6084/m9.figshare.7075487
title: The frontier lines of the constituents of matter

description: Abstract Looking at the chart of nuclides as presented at the URL of the International Atomic Energy Agency (IAEA) [1] – that, one supposes, contains all the known nuclides, the natural and the artificial ones – one verifies the existence of two delimiting lines admitted to be the borders of the constituents of matter. One assumes that the lighter atoms have been formed in a nucleosynthesis process within the core of certain stars while the heavier were produced during the implosion of old stars, a supernova or in the fusion of two neutron stars. Making use of the nuclear semiempirical mass formula along with the energy-time uncertainty relation of quantum mechanics, we show that the calculated border lines show an appreciable agreement with the data obtained from the Chart of Nuclides, the nuclides that are situated far away from those that define the line, or valley, of stability. We also briefly discuss, qualitatively, what is currently known about the astrophysical nucleogenesis processes of the heavier nuclei and their relation with the border lines.
DOI: 10.6084/m9.figshare.7075490.v1
title: Microbiological analysis of contact lens cases: impact of the hospital environment

description: ABSTRACT Purpose: The aim of this study was to analyze the bacterial and fungal microbiota found in contact lens cases among two groups of patients to correlate the data on the habits of contact lens users and to evaluate whether there is a difference in the culture results between users of ridged and nonridged contact lens cases. Methods: Two groups (35 patients per group) were included, consisting of hospital workers and those who had not visited a hospital in the past 30 days, and a questionnaire regarding epidemiological data and habits related to contact lens and lens case use was administered. In addition, 140 samples collected from the right and left compartments of each lens case by swabbing the bottom of the wells were tested using bacterioscopy as well as fungal and bacterial cultures via computerized identification of the species. Results: No fungal growth was identified in any of the 70 contact lens cases; however, bacteria were found in 39 cases, and there was no statistical difference between the groups. Most bacteria (&gt;85%) were gram-negative bacilli. Contamination inone compartment of the contact lens case elevated the risk of contamination of the other side (&gt;80%). Moreover, contamination was statistically higher in the ridged cases than in nonridged cases (p=0.0149). Conclusion: The types of bacteria contaminating the cases are generally not seen in eye diseases associated with contact lens use, suggesting that other decisive variables are involved in eye infection from a contaminated lens or case. Fungal contamination of contact lens cases appears to be an exception. Ridged cases are commonly used by contact lens wearers and present a potential risk to eye health. In addition, the results of bacterial tests between hospital workers and those who did not visit a hospital were not significantly different.
DOI: 10.26165/juelich-data/tvwuup/ojmuiv
title: 203.png

description: :unav
DOI: 10.6084/m9.figshare.7075484
title: Influence of crusher type in the shape of fine crushed aggregate grains

description: Abstract Quarries have invested in equipment to increase production and improve the quality of their products, such as vertical shaft impact crushers (VSI). This type of crusher works with autogenous comminution of the material to improve the shape of the coarse aggregates. However, there are few studies about the influence of crushers in the shape of fine aggregate grains. In this context, gneiss and granite fine crushed aggregates, produced in cone crushers and VSI were studied. Parameters such as coefficient of volumetric shape, aspect ratio, and circularity were used to compare these aggregates with the river sand. The results showed that there is a difference between the shape of the sand river and the fine crushed aggregates. Among the crushed aggregates, those from the VSI showed improvements in grain shape, compared to aggregates from cone crushers. However, this improvement decreases with the reduction of grain size. Mortars produced with the studied aggregates were also evaluated. It was verified their influence on the consistency, the air content and compressive strength at 28 days. For the study in the mortar, the crushed aggregates were separated in fractions by sieving and composed to obtain the same granulometric distribution for all the aggregates. The mortars made with the crushing aggregates from the VSI showed higher fluidity, lower air content and higher compressive strength when compared to the crushed aggregates produced in the cone crusher.
DOI: 10.6084/m9.figshare.7075487.v1
title: The frontier lines of the constituents of matter

description: Abstract Looking at the chart of nuclides as presented at the URL of the International Atomic Energy Agency (IAEA) [1] – that, one supposes, contains all the known nuclides, the natural and the artificial ones – one verifies the existence of two delimiting lines admitted to be the borders of the constituents of matter. One assumes that the lighter atoms have been formed in a nucleosynthesis process within the core of certain stars while the heavier were produced during the implosion of old stars, a supernova or in the fusion of two neutron stars. Making use of the nuclear semiempirical mass formula along with the energy-time uncertainty relation of quantum mechanics, we show that the calculated border lines show an appreciable agreement with the data obtained from the Chart of Nuclides, the nuclides that are situated far away from those that define the line, or valley, of stability. We also briefly discuss, qualitatively, what is currently known about the astrophysical nucleogenesis processes of the heavier nuclei and their relation with the border lines.
DOI: 10.26165/juelich-data/tvwuup/qidhmt
title: 599.png

description: :unav
DOI: 10.6084/m9.figshare.7075484.v1
title: Influence of crusher type in the shape of fine crushed aggregate grains

description: Abstract Quarries have invested in equipment to increase production and improve the quality of their products, such as vertical shaft impact crushers (VSI). This type of crusher works with autogenous comminution of the material to improve the shape of the coarse aggregates. However, there are few studies about the influence of crushers in the shape of fine aggregate grains. In this context, gneiss and granite fine crushed aggregates, produced in cone crushers and VSI were studied. Parameters such as coefficient of volumetric shape, aspect ratio, and circularity were used to compare these aggregates with the river sand. The results showed that there is a difference between the shape of the sand river and the fine crushed aggregates. Among the crushed aggregates, those from the VSI showed improvements in grain shape, compared to aggregates from cone crushers. However, this improvement decreases with the reduction of grain size. Mortars produced with the studied aggregates were also evaluated. It was verified their influence on the consistency, the air content and compressive strength at 28 days. For the study in the mortar, the crushed aggregates were separated in fractions by sieving and composed to obtain the same granulometric distribution for all the aggregates. The mortars made with the crushing aggregates from the VSI showed higher fluidity, lower air content and higher compressive strength when compared to the crushed aggregates produced in the cone crusher.
DOI: 10.26165/juelich-data/tvwuup/8sofs6
title: 527.png

description: :unav
DOI: 10.6084/m9.figshare.7075472
title: De novo transcriptome assembly of the lobster cockroach Nauphoeta cinerea (Blaberidae)

description: Abstract The use of Drosophila as a scientific model is well established, but the use of cockroaches as experimental organisms has been increasing, mainly in toxicology research. Nauphoeta cinerea is one of the species that has been studied, and among its advantages is its easy laboratory maintenance. However, a limited amount of genetic data about N. cinerea is available, impeding gene identification and expression analyses, genetic manipulation, and a deeper understanding of its functional biology. Here we describe the N. cinerea fat body and head transcriptome, in order to provide a database of genetic sequences to better understand the metabolic role of these tissues, and describe detoxification and stress response genes. After removing low-quality sequences, we obtained 62,121 transcripts, of which more than 50% had a length of 604 pb. The assembled sequences were annotated according to their genes ontology (GO). We identified 367 genes related to stress and detoxification; among these, the more frequent were p450 genes. The results presented here are the first large-scale sequencing of N. cinerea and will facilitate the genetic understanding of the species' biochemistry processes in future works.
DOI: 10.26165/juelich-data/tvwuup/fnfxts
title: 174.png

description: :unav
DOI: 10.6084/m9.figshare.7075469
title: Development of a comprehensive noninvasive prenatal test

description: Abstract Our aim was to develop and apply a comprehensive noninvasive prenatal test (NIPT) by using high-coverage targeted next-generation sequencing to estimate fetal fraction, determine fetal sex, and detect trisomy and monogenic disease without parental genotype information. We analyzed 45 pregnancies, 40 mock samples, and eight mother-child pairs to generate 35 simulated datasets. Fetal fraction (FF) was estimated based on analysis of the single nucleotide polymorphism (SNP) allele fraction distribution. A Z-score was calculated for trisomy of chromosome 21 (T21), and fetal sex detection. Monogenic disease detection was performed through variant analysis. Model validation was performed using the simulated datasets. The novel model to estimate FF was robust and accurate (r2= 0.994, p-value &lt; 2.2e-16). For samples with FF &gt; 0.04, T21 detection had 100% sensitivity (95% CI: 63.06 to 100%) and 98.53% specificity (95% CI: 92.08 to 99.96%). Fetal sex was determined with 100% accuracy. We later performed a proof of concept for monogenic disease diagnosis of 5/7 skeletal dysplasia cases. In conclusion, it is feasible to perform a comprehensive NIPT by using only data from high coverage targeted sequencing, which, in addition to detecting trisomies, also make it possible to identify pathogenic variants of the candidate genes for monogenic diseases.