DOI: 10.5061/dryad.pt11g
title: Data from: A four-component model of the action potential in mouse detrusor smooth muscle cell

description: Detrusor smooth muscle cells (DSMCs) of the urinary bladder are
 electrically connected to one another via gap junctions and form a three
 dimensional syncytium. DSMCs exhibit spontaneous electrical activity,
 including passive depolarizations and action potentials. The shapes of
 spontaneous action potentials (sAPs) observed from a single DSM cell can
 vary widely. The biophysical origins of this variability, and the precise
 components which contribute to the complex shapes observed are not known.
 To address these questions, the basic components which constitute the sAPs
 were investigated. We hypothesized that linear combinations of scaled
 versions of these basic components can produce sAP shapes observed in the
 syncytium. The basic components were identified as spontaneous evoked
 junction potentials (sEJP), native AP (nAP), slow after hyperpolarization
 (sAHP) and very slow after hyperpolarization (vsAHP). The experimental
 recordings were grouped into two sets: a training data set and a testing
 data set. A training set was used to estimate the components, and a test
 set to evaluate the efficiency of the estimated components. We found that
 a linear combination of the identified components when appropriately
 amplified and time shifted replicated various AP shapes to a high degree
 of similarity, as quantified by the root mean square error (RMSE) measure.
 We conclude that the four basic components - sEJP, nAP, sAHP, and vsAHP -
 identified and isolated in this work are necessary and sufficient to
 replicate all varieties of the sAPs recorded experimentally in DSMCs. This
 model has the potential to generate testable hypotheses that can help
 identify the physiological processes underlying various features of the
 sAPs. Further, this model also provides a means to classify the sAPs into
 various shape classes.
description: mouse_detrusor_electrophysiology_intracellular_spontaneous_1khzContains a zip archive of 14 data files obtained from the electrophysiology sessions that recorded spontaneous electrical activities of mouse detrusor muscle cells. The sampling rate is 1 kHz. Two columns without headers (First column gives the time stamp in seconds, and the second column gives voltage in mV)detrusor_intracellular_1k.zipmouse_detrusor_electrophysiology_intracellular_spontaneous_4kHz_set1Contains a zip archive of 4 data files obtained from the electrophysiology sessions that recorded spontaneous electrical activities of mouse detrusor muscle cells. The sampling rate is 4 kHz. Two columns without headers (First column gives the time stamp in seconds, and the second column gives voltage in mV)detrusor_intracellular_4k_set1.zipmouse_detrusor_electrophysiology_intracellular_spontaneous_4kHz_set2Contains a zip archive of 6 data files obtained from the electrophysiology sessions that recorded spontaneous electrical activities of mouse detrusor muscle cells. The sampling rate is 4 kHz. Two columns without headers (First column gives the time stamp in seconds, and the second column gives voltage in mV)detrusor_intracellular_4k_set2.zipmouse_detrusor_electrophysiology_intracellular_spontaneous_4kHz_set3Contains a zip archive of 3 data files obtained from the electrophysiology sessions that recorded spontaneous electrical activities of mouse detrusor muscle cells. The sampling rate is 4 kHz. Two columns without headers (First column gives the time stamp in seconds, and the second column gives voltage in mV)detrusor_intracellular_4k_set3.zip