DOI: 10.5061/dryad.p5hqbzkzk
title: Data from: Amyloid β induces hormetic-like effects through major stress pathways in a C. elegans model of Alzheimer’s disease

description: Amyloid β (Aβ) is a peptide known for its characteristic aggregates in
 Alzheimer’s Disease and its ability to induce a wide range of detrimental
 effects in various model systems. However, Aβ has also been shown to
 induce some beneficial effects, such as antimicrobial properties against
 pathogens. In this work, we explore the influence of Aβ in stress
 resistance in a C. elegans model of Alzheimer’s Disease. We found that C.
 elegans that express human Aβ exhibit increased resistance to heat and
 anoxia, but not to oxidative stress. This beneficial effect of Aβ was
 driven from Aβ in neurons, where the level of induction of Aβ expression
 correlated with stress resistance levels. Transcriptomic analysis revealed
 that this selective stress resistance was mediated by the Heat Shock
 Protein (HSPs) family of genes. Furthermore, neuropeptide signaling was
 necessary for Aβ to induce stress resistance, suggesting neuroendocrine
 signaling plays a major role in activating organismal stress response
 pathways. These results highlight the potential beneficial role of Aβ in
 cellular function, as well as its complex effects on cellular and
 organismal physiology that must be considered when using C. elegans as a
 model for Alzheimer’s Disease.
description: # Data for Amyloid β Induces Hormetic-Like Effects Through Major Stress
 Pathways in a C. elegans Model of Alzheimer’s Disease Data includes
 results from experiments using a C. elegans strain that expresses human
 amyloid-beta fluorescently labeled at substoichiometric ratios. Contact
 Adriana San Miguel ([[asanmig@ncsu.edu](mailto:asanmig@ncsu.edu)]) with
 any questions. This manuscript has been accepted for publication
 (12/2024): Lichty, J.D.; Mane, H., Yarmey, V.R., San Miguel, A. Amyloid β
 Induces Hormetic-Like Effects Through Major Stress Pathways in a C.
 elegans Model of Alzheimer’s Disease. PLOS One (accepted). Data files
 include all data presented in the above-referenced manuscript. ##
 DataSet\_Lichty\_et\_al\_2024.xlsx - Tabs Figure 1B, Figure 1C, Figure 1D
 | variables | units | description | | :------------------- | :---------: |
 :------------------------------------------------------------------------------- | | Date | date | Date experiment was performed | | Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations | | Strain | categorical | Strain used in the experiment | | Technical Replicate | categorical | Subpopulations obtained from the biological replicate | | Alive | number | Animals that crawled spontaneously or upon prodding | | Dead | number | Animals identified as dead by lack of movement upon prodding | | Missing | number | Animals not identified during counting | Strains: N2 = WT JKM2 = Aβ+ JKM3 = Aβ- ## DataSet\_Lichty\_et\_al\_2024.xlsx - Tab Figure 2B, 2C | variables | units | description | | :------------------- | :---------: | :------------------------------------------------------------------------------- | | Date | date | Date experiment was performed | | Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations | | Strain | categorical | Strain used in the experiment | | Time Spent at 25C | number | Duration of exposure to 25C (hours) prior to stress assay | | Technical Replicate | categorical | Subpopulations obtained from the biological replicate | | Alive | number | Animals that crawled spontaneously or upon prodding | | Dead | number | Animals identified as dead by lack of movement upon prodding | | Missing | number | Animals not identified during counting | Strains: N2 = WT CL2355 = nAβ CL4176 = mAβ ## DataSet\_Lichty\_et\_al\_2024.xlsx - Tab Figure 3A | variables | units | description | | :------------------- | :---------: | :------------------------------------------------------------------------------- | | Date | date | Date experiment was performed | | Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations | | Strain | categorical | Strain used in the experiment | | Technical Replicate | categorical | Subpopulations obtained from the biological replicate | | Alive | number | Animals that crawled spontaneously or upon prodding | | Dead | number | Animals identified as dead by lack of movement upon prodding | | Missing | number | Animals not identified during counting | Strains: N2 = WT OH438 = nGFP ## DataSet\_Lichty\_et\_al\_2024.xlsx - Tab Figure 3B | variables | units | description | | :------------------- | :---------: | :------------------------------------------------------------------------------- | | Date | date | Date experiment was performed | | Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations | | Strain | categorical | Strain used in the experiment | | NAC Concentration | number | Concentration of N-acetyl cysteine used in the experiment (mM) | | Technical Replicate | categorical | Subpopulations obtained from the biological replicate | | Alive | number | Animals that crawled spontaneously or upon prodding | | Dead | number | Animals identified as dead by lack of movement upon prodding | | Missing | number | Animals not identified during counting | Strains: N2 = WT JKM2 = Aβ+ JKM3 = Aβ- ## DataSet\_Lichty\_et\_al\_2024.xlsx - Tab Figure 4A, 4B, 4C | variables | units | description | | :-------- | :---------: | :----------------------------------------------------------------------- | | Gene | categorical | Gene assessed in expression assay | | Strain | categorical | Strain used in the experiment | | Count | number | Transcripts obtained for the gene using Nanostring gene expression assay | Strains: N2 = WT JKM2 = Aβ+ JKM3 = Aβ- ## DataSet\_Lichty\_et\_al\_2024.xlsx - Tab Figure 4D | variables | units | description | | :------------------- | :---------: | :------------------------------------------------------------------------------- | | Date | date | Date experiment was performed | | Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations | | Strain | categorical | Strain used in the experiment | | RNAi Treatment | categorical | Gene targeted by RNAi or none (Control) | | Technical Replicate | categorical | Subpopulations obtained from the biological replicate | | Alive | number | Animals that crawled spontaneously or upon prodding | | Dead | number | Animals identified as dead by lack of movement upon prodding | | Missing | number | Animals not identified during counting | Strains: NL2099 = rrf-3(-) ASM35 = Aβ+;rrf-3(-) This strain is a cross of JKM2 and NL2099 generated in this work.