DOI: 10.5061/dryad.g1jwstr2n
title: Data from: Downregulation of sST2, a decoy receptor for interleukin-33, enhances subcutaneous tumor growth in murine pancreatic cancer cells

description: Despite the recent scientific advancements, pancreatic cancer remains the
 7th leading cause of cancer-related mortality. Pancreatic cancer
 progression is closely associated with inflammation, and we previously
 showed that short hairpin RNA–mediated knockdown of sST2 expression, a
 soluble decoy receptor for the proinflammatory molecule interleukin-33
 (IL-33), in mouse Panc02 pancreatic cancer cells reduced malignant growth
 following pancreatic (orthotopic) implantation. Furthermore, this growth
 suppression was accompanied by decreased tumor angiogenesis, reduced
 expression of the neutrophil chemoattractant CXCL3, and a lower number of
 tumor-associated neutrophils (TANs). In contrast to previous results, in
 this study, we showed that IL-33-dependent tumor growth and pulmonary
 metastasis occurred following subcutaneous (ectopic) implantation of
 sST2-knockdown cells. This was associated with a decrease in the levels of
 the anti-inflammatory molecule adiponectin and the number of
 GLUT4-positive cancer-associated adipocytes, as well as an increase in
 IκBα phosphorylation levels, Cxcl3 expression, and the accumulation of
 infiltrating CD206+ protumor N2 TANs. Taken together, these results
 suggest that Panc02 cell-derived sST2 differentially affects malignant
 growth in the tumor microenvironment depending on the implantation site.
description: Differentially expressed chemokine and cytokine genes in
 Panc02-shCont and Panc02-sh#5 tumors were analyzed using a Mouse Cytokines
 & Chemokines RT<sup>2</sup> Profiler PCR Array (QIAGEN
 #PAMM-150Z).
description: # Data from: Downregulation of sST2, a decoy receptor for interleukin-33,
 enhances subcutaneous tumor growth in murine pancreatic cancer cells
 [https://doi.org/10.5061/dryad.g1jwstr2n](https://doi.org/10.5061/dryad.g1jwstr2n) This dataset corresponds to Fig. S4 of the manuscript titled *"Downregulation of sST2, a decoy receptor for interleukin-33, enhances subcutaneous tumor growth in murine pancreatic cancer cells."* Fig. S4 presents the cytokine and chemokine gene expression profiles obtained using a PCR array in Panc02 tumors with or without sST2 knockdown. The submitted files include: **Gene_table.csv**: Primer positions and gene information used in the PCR array, including Unigene and RefSeq IDs, gene symbols, descriptions, and official gene names. **Ct_table.csv**: Raw cycle threshold (Ct) values detected for each gene in control (shCont) and sST2-knockdown (sh#5) Panc02 tumors. **Delta_Ct_table.csv**: Normalized Ct values using *Gapdh* as the internal control, and ΔΔCt values representing gene expression changes between shCont and sh#5 groups. ## Description of the data and file structure ### **Gene_table** * **Position**: Location of each primer on the 96-well PCR array plate. * **Unigene**: Cluster ID from the Unigene database, representing transcripts derived from the same gene. Some wells (H06–H12) contain experimental controls and therefore have no corresponding Unigene ID; these were indicated as N/A. * **Refseq**: Reference sequence ID provided by NCBI ([https://www.ncbi.nlm.nih.gov/](https://www.ncbi.nlm.nih.gov/)). * **Symbol**: Abbreviated gene symbol. * **Description**: Brief description of the gene’s function or characteristics. * **Gene name**: Official full gene name. ### **Ct_table** * **Position**: Well location on the 96-well PCR array plate. * **Symbol**: Abbreviated gene symbol. * **shCont (Ct)**: Ct value detected in PCR array using Panc02 mouse pancreatic tumors expressing control shRNA (non-sST2-deficient). Ct values above 35 are considered undetectable and are marked as ND. * **sh#5 (Ct)**: Ct value detected in PCR array using Panc02 mouse pancreatic tumors with sST2 knockdown via shRNA. ### **Delta_Ct_table** * **Position**: Location on the 96-well PCR array plate. * **Symbol**: Abbreviated gene symbol. * **shCont (Ct(GOI) - Ct(Gapdh))**: Normalized Ct value for the gene of interest (GOI) in Panc02 tumors expressing control shRNA (non-sST2-deficient), using Gapdh as the internal control. N/A indicates that the Ct value was undetectable and normalization could not be performed. * **sh#5 (Ct(GOI) - Ct(Gapdh))**: Normalized Ct value for the GOI in Panc02 tumors with sST2 knockdown. * **Delta(Ct)**: Difference between normalized Ct values in shCont and sh#5 groups. This reflects the change in gene expression in Panc02 tumors due to sST2 deficiency. N/A indicates that the Ct value was undetectable and the calculation could not be performed.