cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0h993rq
title: CIL:45985, Homo sapiens. In Cell Image Library

description: Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities.

The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.
description: Plate 26007 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.
 
Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).
 
This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.
DOI: 10.15468/dl.4x2bag
title: Occurrence Download

description: A dataset containing 111006 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Europe",
 "Country is one of (France, Spain, Portugal, Italy, Greece)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Month is one of (April, May)",
 "OccurrenceStatus is Present",
 "TaxonKey is Cistus",
 "Year 1989-2025"
 ]
}

 The dataset includes 111006 records from 422 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009181-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0nk3d43
title: CIL:8463, Rattus, multipolar neuron. In Cell Image Library

description: Development of axon and dendritic arbors in cultured hippocampal neurons after 2 days in vitro. At this timepoint, many, but not all cells are polarized, evidenced by the absence of a long, slender axon. MAP2 staining (red) is becoming segregated to the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Nonneuronal cells (e.g. glia) are infrequent, but may also be present in this preparation. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 40X (HCX PL APO, NA 1.25) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. This merged image was generated with the MetaMorph color combine function.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
DOI: 10.15468/dl.f8agtf
title: Occurrence Download

description: A dataset containing 11 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((85.00601 -10.69239,130.99399 -10.69239,130.99399 7.69239,85.00601 7.69239,85.00601 -10.69239))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Lithocarpus platycarpus"
 ]
}

 The dataset includes 11 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009205-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0st7nvk
title: CIL:8462, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Development of axon and dendritic arbors in cultured hippocampal neurons after 2 days in vitro. At this timepoint, many, but not all cells are polarized, evidenced by the absence of a long, slender axon. MAP2 staining (red) is becoming segregated to the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Nonneuronal cells (e.g. glia) are infrequent, but may also be present in this preparation. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 40X (HCX PL APO, NA 1.25) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. This merged image was generated with the MetaMorph color combine function.
DOI: 10.6075/j08k77nv
title: CIL:45966, Homo sapiens. In Cell Image Library

description: Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities.

The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.
description: Plate 25965 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.
 
Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).
 
This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.
DOI: 10.6075/j0vt1r56
title: CIL:8470, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Development of axon and dendritic arbors in cultured hippocampal neurons after 2 days in vitro. At this timepoint, many, but not all cells are polarized, evidenced by the absence of a long, slender axon. MAP2 staining (red) is becoming segregated to the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Nonneuronal cells (e.g. glia) are infrequent, but may also be present in this preparation. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 40X (HCX PL APO, NA 1.25) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. This merged image was generated with the MetaMorph color combine function.
DOI: 10.6075/j0vd6zgg
title: CIL:847, Rattus, multipolar neuron, hippocampal neuron in vitro. In Cell Image Library

description: Neuron expressing soluble CFP at 14 days in vitro. By this time point, cultured hippocampal neurons have developed a morphology typical of mature neurons with an extensive axonal arbor, a tapered and branched dendritic arbor, and synaptic connections. The dendritic arbor is studded with spines, specialized structures commonly associated with excitatory synapses, and growth cones may be seen at the ends of axonal branches. 

Embryonic rat hippocampal neurons were prepared and transfected with soluble CFP (excitation, 439; emission, 476) at plating (Dotap, Roche) as previously described (Kaech and Banker, 2006, Nat Protoc), fixed for 15 minutes (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, warmed to 37°C, permeabilized with 0.25% Triton X-100 for 7 minutes, and rinsed with phosphate buffered saline at 14 days in vitro (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Images were acquired with a Leica DMRXA microscope with a mercury arc lamp, a 16X PL Fluotar (0.5 NA) oil immersion lens, a Leica CFP filter cube, Princeton Instruments Micromax CCD camera and MetaMorph software. This image shows a merged view of: A) CFP expression throughout the extent of a single expressing neuron in the field; B) identical field imaged with phase microscopy. Image was generated with the MetaMorph overlay function, and the single plane fluorescence view is included in this image group.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: in-focus phase contrast
Visualization methods: Cyan fluorescent proteins from Aequorea
Processing history: recorded image
DOI: 10.15468/dl.rexxse
title: Occurrence Download

description: A dataset containing 12 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is CHN.28_1",
 "TaxonKey is Astragalus dilutus"
 ]
}

 The dataset includes 12 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009206-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.y2guyv
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is CHN.28_1",
 "TaxonKey is Ipomoea nil"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009207-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.17489775
title: ФИНАНСОВЫЕ ТЕХНОЛОГИИ КАК ДРАЙВЕР КОНКУРЕНТОСПОСОБНОСТИ КОММЕРЧЕСКИХ БАНКОВ УЗБЕКИСТАНА: ОТ АВТОМАТИЗАЦИИ ОПЕРАЦИЙ К ИНТЕЛЛЕКТУАЛЬНОЙ АНАЛИТИКЕ

description: В статье рассматривается роль финансовых технологий (финтех) в повышении конкурентоспособности коммерческих банков Узбекистана. Исследование сосредоточено на переходе от стадий автоматизации банковских операций к использованию интеллектуальной аналитики, включая искусственный интеллект, большие данные и открытые банковские API. На основании анализа официальных данных Центральный банк Республики Узбекистан (CBU), статистики интернет-покрытия и цифровой инфраструктуры, а также кейсов ведущих финтех-и банковских игроков (таких как Click, Uzum Bank, TBC Bank Uzbekistan), выделены три ключевые составляющие конкурентного преимущества: 1) расширение цифровых платежных и платёжно-операционных сервисов (инстант-платежи, QR/NFC, интеграция с глобальными системами); 2) внедрение интеллектуальной аналитики — скоринг, персонификация, антифрод-системы, прогнозная аналитика; 3) регулирование и институциональные реформы — создание правовой и технологической базы для финтех-эко­системы, открытого банкинга и цифровой инклюзии. Установлено, что банковские организации, опережающие по уровню цифровизации и способности к аналитике, демонстрируют более высокую операционную эффективность, рост клиентской базы и укрепление конкурентных позиций. В статье выработаны рекомендации для коммерческих банков Узбекистана по стратегическому использованию финтех-инструментов, управлению рисками и оптимизации бизнес-моделей. Работа актуальна в контексте глобальной цифровой трансформации финансовой системы и задач устойчивого развития банковского сектора Узбекистана.
DOI: 10.5281/zenodo.17489776
title: ФИНАНСОВЫЕ ТЕХНОЛОГИИ КАК ДРАЙВЕР КОНКУРЕНТОСПОСОБНОСТИ КОММЕРЧЕСКИХ БАНКОВ УЗБЕКИСТАНА: ОТ АВТОМАТИЗАЦИИ ОПЕРАЦИЙ К ИНТЕЛЛЕКТУАЛЬНОЙ АНАЛИТИКЕ

description: В статье рассматривается роль финансовых технологий (финтех) в повышении конкурентоспособности коммерческих банков Узбекистана. Исследование сосредоточено на переходе от стадий автоматизации банковских операций к использованию интеллектуальной аналитики, включая искусственный интеллект, большие данные и открытые банковские API. На основании анализа официальных данных Центральный банк Республики Узбекистан (CBU), статистики интернет-покрытия и цифровой инфраструктуры, а также кейсов ведущих финтех-и банковских игроков (таких как Click, Uzum Bank, TBC Bank Uzbekistan), выделены три ключевые составляющие конкурентного преимущества: 1) расширение цифровых платежных и платёжно-операционных сервисов (инстант-платежи, QR/NFC, интеграция с глобальными системами); 2) внедрение интеллектуальной аналитики — скоринг, персонификация, антифрод-системы, прогнозная аналитика; 3) регулирование и институциональные реформы — создание правовой и технологической базы для финтех-эко­системы, открытого банкинга и цифровой инклюзии. Установлено, что банковские организации, опережающие по уровню цифровизации и способности к аналитике, демонстрируют более высокую операционную эффективность, рост клиентской базы и укрепление конкурентных позиций. В статье выработаны рекомендации для коммерческих банков Узбекистана по стратегическому использованию финтех-инструментов, управлению рисками и оптимизации бизнес-моделей. Работа актуальна в контексте глобальной цифровой трансформации финансовой системы и задач устойчивого развития банковского сектора Узбекистана.
DOI: 10.6075/j0db80k8
title: CIL:13377, Homo sapiens. In Cell Image Library

description: Antibodies were rabbit anti-HEC1 (green) antibody (Abcam) used at 1:1,000. and sheep anti-MCAK (red) (Andrews et al., 2004) used at 1 μg/ml, and DNA (blue). Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Corresponds to Fig 5B in JCB vol. 179 no. 2 187-197.
description: z-focal series through Hela cell in metaphase illustrating MCAK localization in Bod-1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.
description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: constrained iterative deconvolution
DOI: 10.6075/j0k35shw
title: CIL:41647, Halammohydra schulzei. In Cell Image Library

description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
description: Confocal micrograph of a Halammohydra schulzei, which is a hydra-type fresh-water cnidarian less than 1 millimeter long. 2011 Olympus BioScapes Digital Imaging Competition©.
DOI: 10.6075/j0sb45z5
title: CIL:54893, Homo sapiens, pyramidal cell. In Cell Image Library

description: This image displays the PHF array.
description: We examined the structural organization of cytoskeletal components and membrane systems in neurons of well preserved biopsy material from Alzheimer’s disease (AD). Information was obtained from thin sectioned material using conventional electron microscopy and from thick sections with the high voltage electron microscope.
description: Imaging mode: Electronic tomography
DOI: 10.6075/j00r9pms
title: CIL:54906, Homo sapiens, pyramidal cell. In Cell Image Library

description: Imaging mode: Electronic tomography
description: We examined the structural organization of cytoskeletal components and membrane systems in neurons of well preserved biopsy material from Alzheimer’s disease (AD). Information was obtained from thin sectioned material using conventional electron microscopy and from thick sections with the high voltage electron microscope.
description: This image displays the PHF array.
DOI: 10.6075/j0js9qnz
title: CIL:55777, Homo sapiens, SW481. In Cell Image Library

description: Imaging mode: Apotome Microscopy
Visualization methods: Membrane GFP
description: mGFP transfection
description: SW480 CRC Cell Before Treatment with PMA
DOI: 10.6075/j00000vn
title: CIL:12275, Gallus gallus gallus, memory B cell. In Cell Image Library

description: Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Cytokinesis localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP WT. Endogenous Aurora B is green and DAPI (DNA) is blue. See Supplementary Fig 1E panels 13-16 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed