cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/sh4s9-5y266
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-12/rawdata/</p>20160424_YWC212_20002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-12/rawdata/20160424_YWC212_20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20002_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-12/3dimage_74856/20160424_YWC212_20002_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20002_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-12/3dimage_74857/20160424_YWC212_20002_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mg063-0e212
title: Rhesus Macaque Fibroblast

description: Proteasome inhibition Campbell et al 2008 Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate to any virus because light image only taken in the red channel. Discovered the autofluorescent problem when looking at fibroblasts with no virus, bright puncta everywhere and correlate to onions
description: <strong>Tilt Series Date:</strong> 2015-05-19</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr-Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift. Proteasome inhibition 200mg/ml</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-1/3dimage_57239/</p>tomography10.rec,  Reconstruction (Pixel Size 24.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-1/3dimage_57239/tomography10.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography10.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-1/rawdata/tomography10.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography10_full.rec,  Reconstruction (Pixel Size 0.0 nm), 577.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-05-19-1/3dimage_76013/tomography10_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2p225-0gj37
title: M. xanthus knockout minor pilins

description: Grid box YWC130Strain SA6815
description: <strong>Tilt Series Date:</strong> 2015-04-26</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-1/rawdata/</p>20150426_YWC130_10007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-1/rawdata/20150426_YWC130_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150426_YWC130_10001_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-1/3dimage_54842/20150426_YWC130_10001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20150426_YWC130_10007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-26-1/3dimage_54852/20150426_YWC130_10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zmhzr-1jf83
title: HeLa cells expressing TRIM5α-YFP

description: HeLa cell line 25 expressing stable TRIM5α-YFP. Correlated lightelectron microscopy (11062015Tom). Concentration of 500 nmblue beads (phosphorex) 4/150. 20 nm gold highly concentrated.Manual blotting with Whatman 40.HeLa cell line 25 expressingstable TRIM5α-YFP.
description: <strong>Tilt Series Date:</strong> 2015-10-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cell line 25 and VSV-G pseudotyped virus Vpr-Tomato provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells infected with pseudovirus Vpr-Tomato (in box). 500 µl of media and 500 µl of virus. Incubated with MG-132 (Campbell et al 2008) 6hrs. Cells were washed with warm media and incubated at 37°C before freezing.</p>
<strong>Sample Preparation:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-2/3dimage_71002/</p>tomography10.rec,  Reconstruction (Pixel Size 24.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-2/3dimage_71002/tomography10.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography10.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-2/rawdata/tomography10.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography10_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-2/3dimage_76107/tomography10_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.4w89nw
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Denticetopsis macilenta)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 8 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045039-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/zyect-2md50
title: V. cholerae JAS290 delta-tcpS

description: Classical strain with ctxA deletionStrain name JAS290 (tcpS gene knockout)Cell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2016-08-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Gabriela Kovacikova</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-5/rawdata/</p>20160807_YWC217_10005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-5/rawdata/20160807_YWC217_10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160807_YWC217_10005_part121_22.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-5/3dimage_76463/20160807_YWC217_10005_part121_22.rec"
        > <i class="download icon"></i> Download </a></p>

        20160807_YWC217_10005_part121_2.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-07-5/3dimage_76536/20160807_YWC217_10005_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/95cf9-b0d16
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-22/rawdata/</p>20160424_YWC212_20012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-22/rawdata/20160424_YWC212_20012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20012_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-22/3dimage_74830/20160424_YWC212_20012_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20012_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-22/3dimage_74831/20160424_YWC212_20012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b8023-x9538
title: HeLa cells expressing TRIM5α-YFP

description: HeLa cell line 25 expressing stable TRIM5α-YFP. Correlated lightelectron microscopy (11062015Tom). Concentration of 500 nmblue beads (phosphorex) 4/150. 20 nm gold highly concentrated.Manual blotting with Whatman 40.HeLa cell line 25 expressingstable TRIM5α-YFP.
description: <strong>Tilt Series Date:</strong> 2015-10-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Cell line 25 and VSV-G pseudotyped virus Vpr-Tomato provided by Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells infected with pseudovirus Vpr-Tomato (in box). 500 µl of media and 500 µl of virus. Incubated with MG-132 (Campbell et al 2008) 6hrs. Cells were washed with warm media and incubated at 37°C before freezing.</p>
<strong>Sample Preparation:</strong> HeLa cells expressing TRIM5α-YFP infected Vpr-Tomato HIV-1</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-12/3dimage_71019/</p>tomography4.rec,  Reconstruction (Pixel Size 24.0 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-12/3dimage_71019/tomography4.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography4.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-12/rawdata/tomography4.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography4_full.rec,  Reconstruction (Pixel Size 0.0 nm), 666.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-10-29-12/3dimage_76103/tomography4_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f2m5b-f9n87
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-05-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-41/rawdata/</p>20150511_AK07_10041.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-41/rawdata/20150511_AK07_10041.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10041_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-41/3dimage_56438/20150511_AK07_10041_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20150511_AK07_10041_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-05-11-41/3dimage_56446/20150511_AK07_10041_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.p8w5gs
title: Occurrence Download

description: A dataset containing 434749 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is one of (IND.20.17_1, IND.20.18_1)",
 "OccurrenceStatus is Present",
 "TaxonKey is Aves",
 "Year 2020-2025"
 ]
}

 The dataset includes 434749 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045005-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/mwawp-3nc72
title: Myxococcus xanthus delta-pilY1

description: strain SA6852Grid box YWC212
description: <strong>Tilt Series Date:</strong> 2016-04-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 175.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-18/rawdata/</p>20160424_YWC212_20008.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-18/rawdata/20160424_YWC212_20008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20008_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-18/3dimage_74858/20160424_YWC212_20008_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        20160424_YWC212_20008_full.rec,  Reconstruction (Pixel Size 0.0 nm), 158.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-24-18/3dimage_74859/20160424_YWC212_20008_full.rec"
        > <i class="download icon"></i> Download </a></p>