DOI: 10.5061/dryad.d51c5b03m
title: Data from: Cerebellar complex spikes multiplex complementary behavioral information

description: Purkinje cell (PC) discharge, the only output of cerebellar cortex,
 involves two types of action potentials, high-frequency simple spikes
 (SSs) and low-frequency complex spikes (CSs). While there is consensus
 that SSs convey information needed to optimize movement kinematics, the
 function of CSs, determined by the PC´s climbing fibre input, remains
 controversial. While initially thought to be specialized in reporting
 information on motor error for the subsequent amendment of behavior, CSs
 seem to contribute to other aspects of motor behavior as well. When faced
 with the bewildering diversity of findings and views unraveled by highly
 specific tasks one may wonder if there is just one true function with all
 the other attributions wrong? Or is the diversity of findings a reflection
 of distinct pools of PCs, each processing specific streams of information
 conveyed by climbing fibres? With these questions in mind, we recorded CSs
 from the monkey oculomotor vermis deploying a repetitive saccade task that
 entailed sizable motor errors as well as small amplitude saccades,
 correcting them. We demonstrate that in addition to carrying error-related
 information, CSs carry information on the metrics of both primary and
 small corrective saccades in a time-specific manner, with changes in CS
 firing probability coupled with changes in CS duration. Furthermore, we
 also found CS activity that seemed to predict the upcoming events. Hence
 PCs receive a multiplexed climbing fibre input that merges complementary
 streams of information on the behavior, separable by the recipient PC
 because they are staggered in time.
description: <b>Fig 1.
 The influence of repetitive saccade paradigm on saccade behavior and
 encoding of primary saccade direction by
 CSs</b> <b>Fig 2. CSs encode primary saccade
 amplitude and duration but not peak velocity during the <i>‘early
 post-saccadic period’</i></b>
 <em>(<strong>Note:</strong>
 The values of bootstrapped mean ± confidence intervals in Fig 2B, the
 right panel, are subject to change with every iteration and may not
 perfectly match the values of the original figure in the
 manuscript.)</em> <b>Fig 3.
 CSs encode corrective saccade direction, amplitude and duration during the
 <i>‘post-corrective saccadic period’</i></b>
 <em>(<strong>Note:</strong>
 The values of bootstrapped mean ± confidence intervals in Fig 3D, the
 right panel, are subject to change with every iteration and may not
 perfectly match the values of the original figure in the
 manuscript.)</em> <b>Fig 4.
 CSs encode the retinal error direction and magnitude during the
 <i>‘late post-saccadic period’</i> in a manner different from
 primary and corrective saccades</b> <b>Fig
 5. CS activity during the <i>‘early post-saccadic period’</i>
 carries information on primary saccades and errors, whereas during the
 <i>‘late post-saccadic period’</i> it is mostly
 error-driven</b> <b>Fig 6. Changes in CS
 duration encode changes in primary and corrective saccades as well as
 errors</b> <b>Fig 7. A trial onset related
 CS discharge seems to predict the upcoming events</b>
 <b>Fig 8. Encoding of different task parameters by
 individual PCs</b> <b>SUPPORTING
 INFORMATION</b> <b>S1 Fig. Influence of the
 repetitive saccade task on saccade vigor</b>
 <b>S2 Fig. Relationship of CS response to saccade end, CS
 responses to CF and CP saccades and a comparison of CF and CP saccades
 made in the same direction in an exemplary PC</b>
 <em>(<strong>Note:</strong>
 The values of bootstrapped mean ± confidence intervals in S2 Fig C are
 subject to change with every iteration and may not perfectly match the
 values of the original figure in the
 manuscript.)</em> <b>S3 Fig.
 Analysis of encoding of primary saccade metrics, separately for CSs in
 PD<i><sub>ps</sub></i> and
 PD<i><sub>ps</sub></i>+180°</b>
 <em>(<strong>Note:</strong>
 The values of bootstrapped mean ± confidence intervals in S3 Fig F and
 H are subject to change with every iteration and may not perfectly match
 the values of the original figure in the
 manuscript.)</em> <b>S4 Fig.
 Analysis of encoding of corrective saccade metrics, separately for CSs in
 PD<i><sub>corr</sub></i> and
 PD<i><sub>corr</sub></i>+180°</b>
 <em>(<strong>Note:</strong>
 The values of bootstrapped mean ± confidence intervals in S4 Fig G, H, I
 and J are subject to change with every iteration and may not
 perfectly match the values of the original figure in the
 manuscript.)</em> <b>S5
 Fig.</b> <b>Primary saccades made in the same direction but
 resulting in opposite errors evoke similar CS
 responses</b> <b>S6 Fig. The influence of
 primary saccade amplitude in
 PD<i><sub>ps</sub></i> and
 PD<i><sub>ps</sub></i>+180° with comparable error
 sizes</b> <b>S7 Fig. A multiple regression
 analysis testing the influence of primary saccades and errors on CS
 activity during non-overlapping post-saccadic periods</b>
 <b>S8 Fig. Complementary changes in CS duration and firing
 rate</b>