cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.8hh6g56
title: Data from: On the road: postglacial history and recent expansion of the annual Atriplex tatarica in Europe

description: Aim: The Holocene history of annual plant species is at best shadowy
 because, for most, the palaeobotanical data is scarce or absent. Hence,
 there is limited information on their glacial refugia and postglacial
 colonisation pathways. Also, little is known how human activity has
 affected their expansion. Here, we outline the joint influences of
 postglacial colonisation and recent expansions on the genetic diversity of
 the continental, sub-halophyte species Atriplex tatarica during the late
 Pleistocene and the Holocene. Location: Europe. Taxon: Atriplex tatarica
 (Amaranthaceae) Methods: We analysed 780 individuals from 80 populations
 throughout the current European distribution range, employing chloroplast
 DNA sequences and microsatellite markers. Results: Five haplotype lineages
 were recognised based on the results of the cpDNA phylogenetic analyses.
 These lineages originated 0.43−0.22 my BP. Bayesian clustering analyses
 divided populations of A. tatarica into two clusters: (i) populations in
 the Pannonian Basin and the Bohemian Massif and (ii) populations in the
 North European Plain, the Balkan Peninsula, the Carpathian Arc and the
 Pontic region. The ABC approach provided the strongest statistical support
 for a model proposing refugia in the Balkan Peninsula and the Pannonian
 Basin. Expansions from these refugia occurred around 7,000 yr BP. Main
 conclusion: Atriplex tatarica represents a continental species growing
 exclusively in man-made habitats. It serves as a phylogeographic model for
 annual ruderal taxa currently spreading in central Europe along highways
 and representing a large group of alien plant species, the so-called
 archaeophytes. Atriplex tatarica survived the last glaciation in both a
 southerly located Balkan refugium and in a more northerly refugium in the
 Pannonian Basin. From these, the species has colonised Europe as a result
 of expansion of humans and the anthropogenic climate change. The massive
 colonisation of central north Europe is very recent, with the expansion
 estimated to have occurred only hundreds of years ago.
description: Atriplex_alignment_haplotypesAligned sequences of the 22 cpDNA haplotypes of A. tatarica and one haplotype of A. sagittata as outgroup. The alignment is based on the concatenated sequences of the four analyzed cpDNA regions: rpl32-trnL, psbD-trnT, atpI-atpH, trnD-trnT.Atriplex_alignment_samplesAligned of all cpDNA sequences of A. tatarica and A. sagittata. The alignment is based on the concatenated sequences of the four analyzed cpDNA regions: rpl32-trnL, psbD-trnT, atpI-atpH, trnD-trnT.INSTRUCT_input_fileMicrosatellite genotypes for 41 populations of Atriplex tatarica - input file for program Instruct.microsatellite_genotypesMicrosatellite genotypes for 41 populations of Atriplex tatarica.
DOI: 10.5061/dryad.1j61k78
title: Data from: Nitrogen deposition magnifies the sensitivity of desert steppe plant communities to large changes in precipitation

description: 1. Precipitation alteration and nitrogen (N) deposition caused by
 anthropogenic activities could profoundly affect the structure and
 functioning of plant communities in arid ecosystems. However, the plant
 community impacts conferred by large temporal changes in precipitation,
 especially with a concurrent increase in N deposition, remain unclear. 2.
 To address this uncertainty, from 2016–2017, an in situ field experiment
 was conducted to examine the effects of five precipitation levels, two N
 levels and their interaction on the plant community function and
 composition in a desert steppe in northern China. 3. Aboveground net
 primary production (ANPP) and plant community-weighted mean (CWM) height
 significantly increased with increasing precipitation, and both were well
 fitted with a positive linear model, but with a higher slope under N
 addition. The ANPP increase was primarily driven by the increase in
 Artemisia capillaris, a companion forb sensitive to precipitation
 variation. The plant community composition shifted with precipitation
 enhancement—from a community dominated by Stipa tianschanica, a perennial
 grass, to a community dominated by Artemisia capillaris. 4. Synthesis The
 findings imply that ecosystem sensitivity to future changes in
 precipitation variability will be mediated by two potential mechanisms:
 concurrent N deposition and plant community-level change. It is suggested
 that we should consider the vegetation compositional shift and multiple
 resource colimitation in assessing the sensitivity of terrestrial
 ecosystems to climate change.
description: Ma. et al.
 2019
DOI: 10.5061/dryad.n34035p
title: Data from: Moving beyond the host: unravelling the skin microbiome of endangered Costa Rican amphibians

description: Some neotropical amphibians, including a few species in Costa Rica, were
 presumed to be “extinct” after dramatic population declines in the late
 1980s but have been rediscovered in isolated populations. Such populations
 seem to have evolved a resistance/tolerance to Batrachochytrium
 dendrobatidis (Bd), a fungal pathogen that causes a deadly skin disease
 and is considered one of the main drivers of worldwide amphibian declines.
 The skin microbiome is an important component of the host´s innate immune
 system and is associated with Bd-resistance. However, the way that the
 bacterial diversity of the skin microbiome confers protection against Bd
 in surviving species remains unclear. We studied variation in the skin
 microbiome and the prevalence of putatively anti-Bd bacterial taxa in four
 co-habiting species in the highlands of the Juan Castro Blanco National
 Park in Costa Rica using 16S rRNA amplicon sequencing. Lithobates
 vibicarius, Craugastor escoces, and Isthomohyla rivularis have recently
 been rediscovered, whereas Isthmohyla pseudopuma has suffered population
 fluctuations but has never disappeared. To investigate the life stage at
 which the protective skin microbiome is shaped and when shifts occur in
 the diversity of putatively anti-Bd bacteria, we studied the skin
 microbiome of tadpoles, juveniles and adults of L. vibicarius. We show
 that the skin bacterial composition of sympatric species and hosts with
 distinct Bd-infection statuses differs at the phyla, family, and genus
 level. We detected 94 amplicon sequence variants (ASVs) with putative
 anti-Bd activity pertaining to distinct bacterial taxa, e.g., Pseudomonas
 spp., Acinetobacter johnsonii, and Stenotrophomonas maltophilia.
 Bd-uninfected L. vibicarius harbored 79% more putatively anti-Bd ASVs than
 Bd-infected individuals. Although microbiome composition and structure
 differed across life stages, the diversity of putative anti-Bd bacteria
 was similar between pre- and post-metamorphic stages of L. vibicarius.
 Despite low sample size, our results support the idea that the skin
 microbiome is dynamic and protects against ongoing Bd presence in
 endangered species persisting after their presumed extinction. Our study
 serves as a baseline to understand the microbial patterns in species of
 high conservation value. Identification of microbial signatures linked to
 variation in disease susceptibility might, therefore, inform mitigation
 strategies for combating the global decline of amphibians.
description: Amphibian skin
 microbiomeThe folder contains the
 sequencing data (raw 16S rRNA gene fastq sequences from a paired-end run
 on an Illumina MiSeq) used in analyses of the respective
 publication.amphibian_skin_microbiome.tar.gz
DOI: 10.5061/dryad.37rv60b
title: Data from: Catalytic synthesis of n-butyl carboxylate with immobilized ionic liquid based on RSM optimization

description: Catalytic synthesis of n-butyl formate, n-butyl acetate, n-butyl
 propionate and n-butyl butyrate using four synthesized functional ionic
 liquids, 1-(3-sulfonapropyl)-1-methyl pyrrolidone sulfate hydrogen salt
 ([C3SO3Hnmp]HSO4), 1-(3-sulfopropyl)-1-methylpyrrolidone p-toluene
 sulfonate ([C3SO3Hnmp]CH3SO3H), 1-(3-sulfopropyl)-1-methyl pyrrolidone
 methyl sulfonate ([C3SO3Hnmp]C6H6SO3H), and 1-(3-sulfopropyl)-1-methyl
 pyrrolidone phosphate ([C3SO3Hnmp]H2PO4) was studied. Butyl butyrate was
 selected as the research object. The ionic liquid
 1-(3-sulfopropyl)-1-methylpyrrolidone bisulfate ([C3SO3Hnmp]HSO4), which
 had the best catalytic effect on butyl butyrate, was immobilized and
 tested. The effects of reaction temperature, reaction time, molar ratio of
 acid to alcohol and catalyst dosage were investigated. Response surface
 methodology (RSM) was used to optimize the process conditions, and the
 optimal process conditions were obtained. The supported ionic liquid
 [C3SO3Hnmp]HSO4 maintained high catalytic activity after 5 cycles.
description: Raw data uploaded to
 Dryad
DOI: 10.5061/dryad.8jm0c3n
title: Data from: Epibenthic predators control mobile macrofauna associated with a foundation species in a sub-arctic subtidal community

description: Foundation species (‘FS’) are strong facilitators providing habitat for
 numerous dependent organisms. The communities shaped by FS are commonly
 structured by interplay of facilitation and consumer control. Predators or
 grazers often indirectly determine community structure eliminating either
 FS or their principal competitors. Alternatively, they can prey on the
 dependent taxa directly, which is generally buffered by FS via forming
 complex habitats with numerous refuges. The latter case has been never
 investigated at high latitudes, where consumer control is widely
 considered weak. We manipulated the presence of common epibenthic
 crustacean predators to assess their effect on mobile macrofauna of the
 clusters developed by a FS (barnacle Balanus crenatus and its empty tests)
 in the White Sea shallow subtidal (65° N). While predation pressure on the
 FS itself here is low, the direct effects of a spider crab Hyas araneus
 and a shrimp Spirontocaris phippsii on the associated assemblages were
 unexpectedly strong. Removing the predators did not change species
 diversity, but tripled total abundance and altered multivariate communtity
 structure specifically increasing the numbers of amphipods, isopods (only
 affected by shrimp) and bivalves. Consumer control in the communities
 shaped by FS may not strictly follow the latitudinal predation gradient
 rule.
description: Species abundances in
 experimental unitsThe data contains the
 properties of the experimental units examined and the numbers of
 individuals for all the macrobenthic organisms found within these units
 upon retrieval in the end of the experiments.Raw_data.xls
DOI: 10.5061/dryad.qp44619
title: Data from: Distributions of mammals in Southeast Asia: the role of the legacy of climate and species body mass

description: Aim: Current species distributions are shaped by present and past biotic
 and abiotic factors. Here we assessed whether abiotic factors (habitat
 availability) in combination with past connectivity and a biotic factor
 (body mass) can explain the unique distribution pattern of Southeast Asian
 mammals, which are separated by the enigmatic biogeographic transition
 zone, the Isthmus of Kra (IoK), for which no strong geophysical barrier
 exists. Location: Southeast Asia Taxon: Mammals Methods: We projected
 habitat suitability for 125 mammal species using climate data for the
 present period and for two historic periods: mid-Holocene (6 kya) and last
 glacial maximum (LGM 21 kya). Next, we employed a phylogenetic linear
 model to assess how present species distributions were affected by the
 suitability of areas in these different periods, habitat connectivity
 during LGM and species body mass. Results: Our results show that cooler
 climate during LGM provided suitable habitat south of IoK for species
 presently distributed north of IoK (in mainland Indochina). However, the
 potentially suitable habitat for these Indochinese species did not stretch
 very far southwards onto the exposed Sunda Shelf. Instead, we found that
 the emerged landmasses connecting Borneo and Sumatra provided suitable
 habitat for forest dependent Sundaic species. We show that for species
 whose current distribution ranges are mainly located in Indochina, the
 area of the distribution range that is located south of IoK is explained
 by the suitability of habitat in the past and present in combination with
 the species body mass. Main conclusions: We demonstrate that a strong
 geophysical barrier may not be necessary for maintaining a biogeographic
 transition zone for mammals, but that instead a combination of abiotic and
 biotic factors may suffice.
description: Presence data for 125
 mammal speciesMammal species presence
 data obtained from the IUCN Red List of Threatened Species as explained in
 detail in the main text. For more details on the file contents please see
 Readme file.Presences.7zBackground data for 125 speciesThree background samples for each of the 125 mammal species, obtained as explained in the main text. For details on the file contents please see Readme file.Background.7z
DOI: 10.5061/dryad.v41d698
title: Data from: Tapeworm manipulation of copepod behaviour: parasite genotype has a larger effect than host genotype

description: Compared to uninfected individuals, infected animals can exhibit altered
 phenotypes. The changes often appear beneficial to parasites, leading to
 the notion that modified host phenotypes are extended parasite phenotypes,
 shaped by parasite genes. However, the phenotype of a parasitized
 individual may reflect parasitic manipulation, host responses to
 infection, or both, and disentangling the contribution of parasite genes
 versus host genes to these altered phenotypes is challenging. Using a
 tapeworm (Schistocephalus solidus) infecting its copepod first
 intermediate host, I performed a full-factorial, cross-infection
 experiment with five host and five parasite genotypes. I found that a
 behavioural trait modified by infection, copepod activity, was affected by
 both host and parasite genotype. There was not clear evidence for host
 genotype by parasite genotype interactions. Several observations indicated
 that host behaviour was chiefly determined by parasite genes: 1) all
 infected copepods, regardless of host or parasite genotype, exhibited
 behavioural changes, 2) parasitism reduced the differences among copepod
 genotypes, and 3) within infected copepods, parasite genotype had twice as
 large an effect on behaviour as host genotype. I conclude that the altered
 behaviour of infected copepods primarily represents an extended parasite
 phenotype, and I discuss how genetic variation in parasitic host
 manipulation could be maintained.
description: Copepod behavior via
 auto trackingSee README for column
 descriptionsbehav_autotracked.csvCopepod behavior via manual trackingSee README for column descriptionsbehav_manuallytracked.csvInfection dataSee README for column descriptionsinfection_data.csv
DOI: 10.5061/dryad.1m3tj32
title: Data from: Floral volatiles structure plant-pollinator interactions in a diverse community across the growing season

description: 1. While the importance of floral odors for pollinator attraction relative
 to visual cues is increasingly appreciated, how they structure
 community-level plant-pollinator interactions is poorly understood.
 Elucidating the functional roles of flowering plant species with respect
 to their floral volatile organic compounds (VOCs) and how those roles vary
 over the growing season is an initial step towards understanding the
 contribution of floral VOCs to plant-pollinator interaction structure. 2.
 We sampled the floral VOCs, phenologies, and bee visitors of
 naturally-growing plants in a montane meadow in the Northern Rocky
 Mountains of USA in order to acquire a base understanding of how floral
 VOCs and other plant traits may structure plant-pollinator interactions
 across the growing season. We expected forb species with floral VOCs that
 were original (far from the community mean) and unique (far from the
 nearest neighbor) would have few pollinating partners (i.e., specialists),
 while forbs with non-original or highly variable floral VOCs would form
 the generalist core of interactors, thereby contributing to network
 nestedness (specialists interacting with nested subsets of generalists).
 Network modularity (patterns of distinct, highly connected subnetworks)
 could be influenced by groups of pollinators that are attracted to or
 repelled by certain floral bouquets. 3. Species blooming in early spring
 emitted similar floral VOC blends containing generalist attractants,
 whereas floral VOC complexity was highest in mid to late summer. Forb
 species varied in the originality, uniqueness, and intraspecific variation
 (i.e., dispersion) of their floral VOCs, indicating the potential for
 different functional roles in plant-pollinator networks. Specifically, the
 originality, uniqueness, and dispersion of forb species’ floral VOCs
 increased across the growing season. 4. Floral VOCs influenced forb
 interactions with pollinators. Floral VOCs contributed to the nested
 structure, but not modular structure, of community-level plant-pollinator
 network structure. Forb species with more original floral VOCs were less
 connected, while forb species emitting more compounds and with higher
 intraspecific variation in floral VOCs were more connected to pollinators.
 5. These findings show that floral scent plays important roles in
 structuring bee-forb interactions and guiding seasonal patterns in complex
 communities. Understanding seasonal patterns in floral VOCs may have
 important implications for plant-pollinator interactions among communities
 differing in species composition, or as shifts occur in suites of
 co-flowering species due to climate change.
description: mtellis_voc_dryad_FEField-collected floral VOCs of individuals of forb species (plant column)mtellis_forb_info_dryad_FEInformation for each forb species, including family, date of peak flower, flower color, floral abundance, and flowering duration.
DOI: 10.5061/dryad.7qr55jn
title: Data from: Frequent fires prime plant developmental responses to burning

description: Coping with temporal variation in fire requires plants to have plasticity
 in traits that promote persistence, but how plastic responses to current
 conditions are affected by past fire exposure remains unknown. We
 investigate phenotypic divergence between populations of four resprouting
 grasses exposed to differing experimental fire regimes (annually-burnt or
 unburnt for &gt;35 years), and test whether divergence persists after
 plants are grown in a common environment for one year. Traits relating to
 flowering and biomass allocation were measured before plants were
 experimentally burnt, and their regrowth was tracked. Genetic
 differentiation between populations was investigated for a subset of
 individuals. Historic fire frequency influenced traits relating to
 flowering and below-ground investment. Previously burnt plants produced
 more inflorescences and invested proportionally more biomass below ground,
 suggesting greater capacity for recruitment and resprouting than unburnt
 individuals. Branch-scale regrowth rate did not differ between treatments,
 but prior fire exposure enhanced total regrown biomass in two species. We
 found no consistent genetic differences between populations suggesting
 trait differences arose from developmental plasticity. Grass development
 is influenced by prior fire exposure, independent of current environmental
 conditions. This priming response to fire, resulting in adaptive trait
 changes, may produce communities more resistant to future fire regime
 changes.
description: Individual level trait
 valuesTrait data for
 individuals. MC = moisture content; FM = fresh mass; DM = dry
 massTrait.data.xlsx
DOI: 10.18126/faxs-ga32
title: Peeling adhesion of DGEBA-Tris polymer to mica
DOI: 10.5061/dryad.p19fp25
title: Data from: Reproductive isolation due to prezygotic isolation and postzygotic cytoplasmic incompatibility in parasitoid wasps

description: The reproductive barriers that prevent gene flow between closely related
 species are a major topic in evolutionary research. Insect clades with
 parasitoid life-style are among the most species-rich insects and new
 species are constantly described, indicating that speciation occurs
 frequently in this group. However, there are only very few studies on
 speciation in parasitoids. We studied reproductive barriers in two
 lineages of Lariophagus distinguendus (Chalcidoidea: Hymenoptera), a
 parasitoid wasp of pest beetle larvae that occur in human environments.
 One of the two lineages occurs in households preferably attacking larvae
 of the drugstore beetle Stegobium paniceum (“DB-lineage”), the other in
 grain stores with larvae of the granary weevil Sitophilus granarius as
 main host (“GW-lineage”). Between two populations of the DB-lineage we
 identified slight sexual isolation as intraspecific barrier. Between
 populations from both lineages, we found almost complete sexual isolation
 caused by female mate choice, and postzygotic isolation, which is
 partially caused by cytoplasmic incompatibility induced by so far
 undescribed endosymbionts which are not Wolbachia or Cardinium. Because
 separation between the two lineages is almost complete, they should be
 considered as separate species according to the biological species
 concept. This demonstrates that cryptic species within parasitoid
 Hymenoptera also occur in Central Europe in close contact to humans.
description: original
 dataExcel file containing
 original data data from the studyoriginal_data.xlsx
DOI: 10.5061/dryad.kr25r48
title: Data from: Variation in chick-a-dee call sequences, not in the fine structure of chick-a-dee calls, influences mobbing behaviour in mixed-species flocks

description: When animals vocalize under the threat of predation, variation in the
 structure of calls can play a vital role in survival. The chick-a-dee
 calls of chickadees and titmice provide a model system for studying
 communication in such contexts. In previous studies, birds’ responses to
 chick-a-dee calls covaried with call structure, but also with unmeasured
 and correlated parameters of the calling sequence, including duty cycle
 (the proportion of the calling sequence when a signal was present). In
 this study, we exposed flocks of Black-capped Chickadees (Poecile
 atricapillus) and heterospecific birds to playback of chick-a-dee calls
 and taxidermic models of predators. We quantified birds’ responses to
 variation in number of D-notes and duty cycle of the signalling sequence.
 Chickadees and heterospecific birds responded more intensely to
 high-duty-cycle treatments, and equally to treatments where duty cycle was
 held constant and the number of D-notes varied. Although our study does
 not disentangle the effects of call rate and duty cycle, it is the first
 to investigate independently the behavioural responses of birds to
 variation in structural and sequence-level parameters of the chick-a-dee
 call during a predator confrontation. Critically, our results confirm that
 the pattern previously observed in a feeding context holds true in a
 mobbing context: variation in calling sequences, not in call structure, is
 the salient acoustic feature of chick-a-dee calls. These results call into
 question the idea that chick-a-dee call structure carries allometric
 information about predator size, suggesting instead that sequence-level
 parameters play a central role in communication in a mobbing context.
description: Landsborough et al DATA
 FOR DRYADInformation containing
 the treatment, species, measures of response, and the conspecific and
 heterospecific factors summarizing the physical responses to playback,
 extracted using PCA.