DOI: 10.5061/dryad.sr0g5s1
title: Data from: The cell uptake properties and hyperthermia performance of Zn0.5 Fe 2.5O4 /SiO2 nanoparticles as magnetic hyperthermia agents

description: Zn0.5Fe2.5O4 nanoparticles (NPs) of 22nm are synthesized by one-pot
 approach and coated with silica for magnetic hyperthermia agents. The
 nanoparticles exhibit superaramagnetic characteristics, high specific
 absorption rate (SAR) (1083 wg-1, f=430 kHz, H=27 kAm-1), large saturation
 magnetization (Ms=85emug-1), excellent colloidal stability and low
 cytotoxicity. The cell uptake properties have been investigated by
 Prussian blue staining, transmission electron microscope (TEM) and
 inductively coupled plasma mass spectrometer (ICP-MS), which resulted in
 time dependent as well as concentration dependent internalization. The
 internalization appeared between 0.5h to 2h, the NPs were mainly located
 in the lysosomes and kept good dispersion after incubation with human
 osteosarcoma MG-63 cells. Then, the relationship between cell uptake and
 magnetic hyperthermia performance was studied. Our results showed that the
 hyperthermia efficiency rest with the amount of internalized NPs, which
 was depended on the concentration and incubation time. Interestingly, the
 NPs could still induce tumor cells to apoptosis/necrosis when
 extracellular NPs were rinsed, but the cell kill efficiency is lower than
 no rinse groups, which indicate that local temperature rise rather than
 mechanical mechanism is the main factor that induce tumor cells death. Our
 findings suggest that this high SAR and biocompatible silica coating
 Zn0.5Fe2.5O4 nanoparticles could serve as a new agents for magnetic
 hyperthermia.
description: Colloidal stability of
 Zn0.5Fe2.5O4/SiO2 nanoparticles Figure S1. Photograph of
 Zn0.5Fe2.5O4/SiO2 aqueous dispersions (5mgNPs ml-1), the sample are stable
 for 72h with no precipitation. Collidal
 stability.tif Zeta potential and size
 distribution of Zn0.5Fe2.5O4/SiO2 nanoparticles Figure S2. (a) Zeta
 potential curve of Zn0.5Fe2.5O4/SiO2 nanoparticles. (b) Size distribution
 by volume of Zn0.5Fe2.5O4/SiO2 nanoparticles in aqueous dispersions
 (1mgNPs ml-1) Figure S2 showed that the zeta potential(Fig.S2 a) of
 Zn0.5Fe2.5O4/SiO2 nanoparticles is about -20 mV and the average
 size(Fig.S2 b) of NPs in aqueous dispersion is about 158.3±41.2 nm,
 demonstrating their colloidal stability, which is vital when they were
 used as magnetic fluids in tumor hyperthermia. Zeta Potential Distribution and
 Size Distribution by Volume.tif The dispersity of
 Zn0.5Fe2.5O4/SiO2 nanoparticles in tumor cells Figure S3. The high
 resolution TEM of Zn0.5Fe2.5O4/SiO2 nanoparticles in MG-63 cells. (a)
 100nm. (b) 200nm. As shown in Figure S3, the NPs kept good dispersion in
 the lysosome at high resolution TEM image, which indicate the NPs still
 able to heat up and damage the tumor cells and thus they are still
 functional as heating meditators. High resolution
 TEM.tif The original heating
 data of Zn0.5 Fe 2.5O4 /SiO2 The original heating
 data of Zn0.5 Fe 2.5O4 /SiO2 Heating data.xls
 
 The SAR of Zn0.5
 Fe 2.5O4 /SiO2 The SAR of Zn0.5 Fe
 2.5O4 /SiO2 in a AMF(f=430kHz) with different
 frequency(23kA/m,27kA/m). SAR.rar
 
 Cytotoxicity
 ofZn0.5Fe2.5O4-SiO2 The original data of
 cytotoxicity of Zn0.5Fe2.5O4/SiO2 nanoparticles to MEF and human
 osteosarcoma MG-63 cells assessed by CCK-8 assay. Cytotoxicity
 ofZn0.5Fe2.5O4-SiO2.xls