DOI: 10.5061/dryad.k5k30
title: Data from: Monocyte recruitment to the dermis and differentiation to dendritic cells increases the targets for Dengue virus replication

description: Dengue virus (DENV) causes the most prevalent arthropod-borne viral
 disease in humans. Although Aedes mosquitoes transmit DENV when probing
 for blood in the skin, no information exists on DENV infection and immune
 response in the dermis, where the blood vessels are found. DENV suppresses
 the interferon response, replicates, and causes disease in humans but not
 wild-type mice. Here, we used mice lacking the interferon-α/β receptor
 (Ifnar–/–), which had normal cell populations in the skin and were
 susceptible to intradermal DENV infection, to investigate the dynamics of
 early DENV infection of immune cells in the skin. CD103+ classical
 dendritic cells (cDCs), Ly6C– CD11b+ cDCs, and macrophages in the
 steady-state dermis were initial targets of DENV infection 12-24 hours
 post-inoculation but then decreased in frequency. We demonstrated
 recruitment of adoptively-transferred Ly6Chigh monocytes from wild-type
 and Ifnar–/– origin to the DENV-infected dermis and differentiation to
 Ly6C+ CD11b+ monocyte-derived DCs (moDCs), which became DENV-infected
 after 48 hours, and were then the major targets for virus replication.
 Ly6Chigh monocytes that entered the DENV-infected dermis expressed
 chemokine receptor CCR2, likely mediating recruitment. Further, we show
 that ~100-fold more hematopoietic cells in the dermis were DENV-infected
 compared to Langerhans cells in the epidermis. Overall, these results
 identify the dermis as the main site of early DENV replication and show
 that DENV infection in the skin occurs in two waves: initial infection of
 resident cDCs and macrophages, followed by infection of monocytes and
 moDCs that are recruited to the dermis. Our study reveals a novel viral
 strategy of exploiting monocyte recruitment to increase the number of
 targets for infection at the site of invasion in the skin and highlights
 the skin as a potential site for therapeutic action or intradermal
 vaccination.
description: I_Dermis steadystate DC
 stain IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.I_Dermis steadystate DC
 stain WTFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.I_Dermis steadystate
 Mono stain IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.I_Dermis steadystate
 Mono stain WTFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.I_Epidermis steadystate
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.I_Epidermis steadystate
 WTFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.II_Epidermis 24h ADE
 DENV IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.II_Epidermis 24h PBS
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.II_Epidermis 24h primary
 DENV IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.II_Exp 24h Epidermis
 Compensation MatrixFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.II_Exp 24h Epidermis DENV
 Compensation MatrixIII_Dermis 24h ADE DENV
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.III_Dermis 24h PBS
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.III_Dermis 24h primary
 DENV IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.III_Dermis steadystate
 Ifnar (C inf)File type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.III_Exp 24h Compensation
 Matrix_DermisFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.III_Exp 24h Compensation
 MatrixIV_Dermis 48h ADE DENV
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.IV_Dermis 48h PBS
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.IV_Dermis 48h primary
 DENV IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.IV_Dermis 72h ADE DENV
 IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.IV_Dermis 72h primary
 DENV IfnarFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.IV_Dermis steadystate
 Ifnar (C inf)File type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.IV_Exp 48 & 72h
 Compensation MatrixFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Mono transfer
 Compensation MatrixFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Mono Ifnar to Ifnar
 Compensation MatrixV_Ms1_Dermis mono Tx
 DENV-infected sideFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms1_Dermis mono Tx
 DENV, non-infected sideFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms2_Dermis no Tx
 DENV-infected sideFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms2_Dermis no Tx DENV,
 non-infected sideFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms3_Dermis mono Tx
 steadystate leftFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms3_Dermis mono Tx
 steadystate rightFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms4_Dermis no Tx
 steadystate leftFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.V_Ms4_Dermis no Tx
 steadystate rightFile type: The
 repository includes FSC 3.0 files that were exported from Diva software
 (BD Biosciences) and can be opened with FlowJo software (TreeStar). In
 some cases, an updated compensation matrix is included for a specific
 experiment that should be applied to the FCS files within the FlowJo
 software. Fluorescent channels also indicate the specifically stained
 markers.Schmid Inventory of data
 repositoryRead this PDF for
 instruction on how to read the data files, the experiments and identity of
 the files.