DOI: 10.5061/dryad.98d51
title: Data from: Pleurostylia serrulata and two allied new species from Africa are actually members of the new world Crossopetalum (Celastraceae)

description: Based on phylogenetic analyses using rDNA and plastid sequence data, and
 the examination of morphological characters, we infer that Pleurostylia,
 as currently delimited, is a polyphyletic group. Pleurostylia serrulata
 and two newly described species from Africa are part of the New World
 Crossopetalum lineage. By contrast, Pleurostylia s. s. consists of all
 remaining species, which form a clade nested within a primarily Malagasy
 lineage. We present preliminary evidence that Pleurostylia opposita, the
 most geographically widespread species in the entire Celastraceae family,
 may include cryptic species. Although molecular evidence supports the
 monophyly of Crossopetalum, this genus does not appear to be well defined
 by a single morphological synapomorphy, rather the following combination
 of character states is diagnostic: 4-merous flowers; stamens inserted in
 the sinuses of a ± 4-lobed intrastaminal floral disk; pistil (2- or)
 4-locular with one atropous ovule per locule; stigma conspicuously (2-)
 4-branched; drupe usually single seeded and asymmetric with an excentric
 style remnant; and seeds exarillate with ramified postchalazal bundles
 visible on the surface and with endosperm present. A taxonomic treatment
 of Crossopetalum in Africa is presented, in which the new combination
 Crossopetalum serrulatum is proposed and two new species, C. bokdamii and
 C. mossambicense , are described and their conservation status assessed.
description: Supplementary Figure 1:
 Parsimony-based strict consensus of the 26S rDNA data matrix for the
 Pleurostylia analysis.Parsimony-based strict
 consensus of the 26S rDNA data matrix for the Pleurostylia analysis.
 Jackknife values ≥ 50% are shown above each branchDarbyshire16Jan2016_FIGS1_26S
 rDNA Pleurostylia.jpgSupplementary Figure 2:
 Parsimony-based strict consensus of the ITS rDNA data matrix for the
 Pleurostylia analysis.Parsimony-based strict
 consensus of the ITS rDNA data matrix for the Pleurostylia analysis.
 Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS2_ITS
 rDNA Pleurostylia.jpgSupplementary Figure 3:
 Parsimony-based strict consensus of the rDNA (ITS + 26S) data matrix for
 the Pleurostylia analysis.Parsimony-based strict
 consensus of the rDNA (ITS + 26S) data matrix for the Pleurostylia
 analysis. Jackknife values ≥ 50% are shown above each
 branch.Darbyshire16Jan2016_FIGS3_rDNA
 ITS+26S Pleurostylia.jpgSupplementary Figure 4:
 Parsimony-based strict consensus of the matK data matrix for the
 Pleurostylia analysis. Jackknife values ≥ 50% are shown above each
 branch.Parsimony-based strict
 consensus of the matK data matrix for the Pleurostylia analysis. Jackknife
 values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS4_matK
 Pleurostylia.jpgSupplementary Figure 5:
 Parsimony-based strict consensus of the trnL-F data matrix for the
 Pleurostylia analysis.Parsimony-based strict
 consensus of the trnL-F data matrix for the Pleurostylia analysis.
 Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS5_trnL-F Pleurostylia.jpgSupplementary Figure 6: Parsimony-based strict consensus of the plastid (matK + trnL-F) data matrix for the Pleurostylia analysis.Parsimony-based strict consensus of the plastid (matK + trnL-F) data matrix for the Pleurostylia analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS6_matK+trnL-F Pleurostylia.jpgSupplementary Figure 7: Parsimony-based strict consensus of the molecular data matrix for the Pleurostylia analysis.Parsimony-based strict consensus of the molecular data matrix for the Pleurostylia analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS7_molecular data matrix Pleurostylia.jpgSupplementary Figure 8: Parsimony-based strict consensus of the 26S rDNA data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the 26S rDNA data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS8_26S rDNA Crossopetalum.jpgSupplementary Figure 9: Parsimony-based strict consensus of the ITS rDNA data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the ITS rDNA data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS9_ITS rDNA Crossopetalum.jpgSupplementary Figure 10: Parsimony-based strict consensus of the rDNA (ITS + 26S) data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the rDNA (ITS + 26S) data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS10_rDNA ITS+26S Crossopetalum.jpgSupplementary Figure 11: Parsimony-based strict consensus of the matK data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the matK data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS11_matK Crossopetalum.jpgSupplementary Figure 12: Parsimony-based strict consensus of the trnL-F data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the trnL-F data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS12_trnL-F Crossopetalum.jpgSupplementary Figure 13: Parsimony-based strict consensus of the plastid (matK + trnL-F) data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the plastid (matK + trnL-F) data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS13_plastid matK+trnL-F Crossopetalum.jpgSupplementary Figure 14: Parsimony-based strict consensus of the molecular data matrix for the Crossopetalum analysis.Parsimony-based strict consensus of the molecular data matrix for the Crossopetalum analysis. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS14_molecular data matrix Crossopetalum.jpgSupplementary Figure 15: Parsimony-based strict consensus of the trnL-F data matrix for the Crossopetalum analysis that includes C. bokdamii.Parsimony-based strict consensus of the trnL-F data matrix for the Crossopetalum analysis that includes C. bokdamii. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS15_trnL-F Crossopetalum incl bokdamii.jpgSupplementary Figure 16: Parsimony-based strict consensus of the plastid (matK + trnL-F) data matrix for the Crossopetalum analysis that includes C. bokdamii.Parsimony-based strict consensus of the plastid (matK + trnL-F) data matrix for the Crossopetalum analysis that includes C. bokdamii. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS16_matK+trnL-F Crossopetalum incl bokdamii.jpgSupplementary Figure 17: Parsimony-based strict consensus of the molecular data matrix for the Crossopetalum analysis that includes C. bokdamii.Parsimony-based strict consensus of the molecular data matrix for the Crossopetalum analysis that includes C. bokdamii. Jackknife values ≥ 50% are shown above each branch.Darbyshire16Jan2016_FIGS17_data matrix Crossopetalum incl bokdamii.jpgTable 1: Data-matrix and tree statistics for each of the phylogenetic analyses.Data-matrix and tree statistics for each of the phylogenetic analyses. “CI” = ensemble consistency index (Kluge and Farris 1969) on the most parsimonious tree(s) for the parsimony-informative characters. “RI” = ensemble retention index (Farris 1989).Table 1_data matrix.docx