cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/4r5bj-00986
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-69/rawdata/</p>Ecoli_CONjugation31.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-69/rawdata/Ecoli_CONjugation31.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation31_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-69/3dimage_107143/Ecoli_CONjugation31_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation31.mrc.mdoc,  50.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-69/rawdata/Ecoli_CONjugation31.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/83f8j-jh994
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-86/rawdata/</p>Ecoli_CONjugation47.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-86/rawdata/Ecoli_CONjugation47.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation47_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-86/3dimage_107178/Ecoli_CONjugation47_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation47.mrc.mdoc,  50.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-86/rawdata/Ecoli_CONjugation47.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4cha1-ma347
title: gh2-4_sq2_cell9

description: GH2-4, Sq2, IT 1.1s, phase plate
description: <strong>Tilt Series Date:</strong> 2018-05-16</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 181.0 eV/Å²,  defocus: -2.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h</p>
<strong>Sample Preparation:</strong> Backside manual blotting 5s</p>
  wnj2018-05-16-8_slicer19531.jpg: Purple: periplasmic density wnj2018-05-16-8_slicer19532.jpg: Purple: cellulose fibers wnj2018-05-16-8_slicer19533.jpg: Purple: cellulose fibers wnj2018-05-16-8_slicer19534.jpg: 4 units when the filaments touches the membrane wnj2018-05-16-8_slicer19535.jpg: Cortical sheet wnj2018-05-16-8_slicer19536.jpg: Transversal cut of site of tight contact between filament and membrane .   .  Purple: periplasmic blob .   .  Green: cellulose
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-8/rawdata/</p>gh2-4_sq2_cell9.mrc,  Tilt Series (Pixel Size 0.43 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-8/rawdata/gh2-4_sq2_cell9.mrc"
        > <i class="download icon"></i> Download </a></p>

        gh2-4_sq2_cell9_part61_7.rec,  Reconstruction (Pixel Size 0.86 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-8/3dimage_92597/gh2-4_sq2_cell9_part61_7.rec"
        > <i class="download icon"></i> Download </a></p>

        gh2-4_sq2_cell9fakesirt30.rec,  Reconstruction (Pixel Size 1.728 nm), 400.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-05-16-8/3dimage_92668/gh2-4_sq2_cell9fakesirt30.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2d8qb-an319
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-102/rawdata/</p>Ecoli_CONjugation61.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-102/rawdata/Ecoli_CONjugation61.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation61_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-102/3dimage_107204/Ecoli_CONjugation61_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation61.mrc.mdoc,  50.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-102/rawdata/Ecoli_CONjugation61.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5ccrb-6zp48
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-80/rawdata/</p>atlas_30027.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-80/rawdata/atlas_30027.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30027_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-80/3dimage_88954/atlas_30027_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30027_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-80/3dimage_90283/atlas_30027_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s5203-p8z57
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-105/rawdata/</p>atlas_20014.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-105/rawdata/atlas_20014.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_20014_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-105/3dimage_89146/atlas_20014_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_20014_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-105/3dimage_90297/atlas_20014_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/45egm-81h41
title: Ecoli_EPEC_8612

description: These are E coli cells (EPEC 8612) obtained from Ritesh (Ilan Rosenshine lab at the Hebrew  .  university). The idea is to image bacterial nanotubes in these cells.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These cells were prepared with Ritesh in Ilan Rosenshine lab at the Hebrew University. </p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. Then washed with PBS and blotted and then plunge . frozen. Yuxi helped in sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-41/rawdata/</p>EPEC_8612_tilt1.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-41/rawdata/EPEC_8612_tilt1.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt1_part36_4.rec,  Reconstruction (Pixel Size 1.796 nm), 501.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-41/3dimage_110699/EPEC_8612_tilt1_part36_4.rec"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt1.mrc.mdoc,  20.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-41/rawdata/EPEC_8612_tilt1.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.34740/kaggle/dsv/12891046
title: Artist Datasets
DOI: 10.22002/bdqpa-yd429
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-8/rawdata/</p>Ecoli_conjugation21.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-8/rawdata/Ecoli_conjugation21.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_conjugation21_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-8/3dimage_107031/Ecoli_conjugation21_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_conjugation21.mrc.mdoc,  50.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-8/rawdata/Ecoli_conjugation21.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xnhh0-2p992
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-116/rawdata/</p>Ecoli_CONjugation74.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-116/rawdata/Ecoli_CONjugation74.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation74_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-116/3dimage_107219/Ecoli_CONjugation74_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation74.mrc.mdoc,  50.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-116/rawdata/Ecoli_CONjugation74.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.7zhr4y
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Erythrogenys erythrogenys",
 "Country is Chinese Taipei",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002168-250827131500795/datasets/export for details.