DOI: 10.6084/m9.figshare.30642659
title: Image Dataset: Photographs of the type series of <i>Najadicola tuzovskiji</i> <b>sp. nov.</b> (Acari: Hydrachnidia: Pionidae) from Northeast Asia

description: This dataset contains raw (uncorrected) photos of selected type specimens of the new mussel-associated mite, <i>Najadicola tuzovskiji</i> Kuznetsova, Kondakov & Bolotov, 2025 (Acari: Hydrachnidia: Pionidae). The species is described in our paper entitled “A new giant mussel-associated mite from Northeast Asia, with an assessment of the phylogenetic position of the genus <i>Najadicola</i> Piersig, 1897 (Acari: Hydrachnidia: Pionidae)” (<i>Ecologica Montenegrina</i>, 92, 36-53 (2025); https://doi.org/10.37828/em.2025.92.4). The type specimens are deposited in the Hydrachnidia collection of the RMBH—Russian Museum of Biodiversity Hotspots, N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, Arkhangelsk, Russia.Additionally, the dataset contains high-resolution figure files from our paper (Figure 1-7).<b>Cite this dataset as follows:</b> Kuznetsova, I. A., Vikhrev, I. V., Konopleva, E. S., Kogut, Y. E., Gofarov, M. Y., Kondakov, A. V. & Bolotov, I. N. (2025) Image Dataset: Photographs of the type series of <i>Najadicola tuzovskiji</i> <b>sp. nov.</b> (Acari: Hydrachnidia: Pionidae) from Northeast Asia. figshare. https://doi.org/10.6084/m9.figshare.30642659<br><b>TYPE SPECIMEN PHOTOS</b><b> (RMBH; by Irina A. Kuznetsova):</b>Abbreviations: d = dorsal view; v = ventral view; gf = genital field(1) <b>Holotype RMBH Hyd_1184 </b>(dissected male specimen on a permanent slide with glycerol gelatin mounting medium) labeled “RUSSIA: Primorsky Kray, 45.8451°N, 134.4623°E, Zavitaya River, a tributary of the Bolshaya Ussurka River, Ussuri River basin, host: <i>Middendorffinaia mongolica</i>, 03 August 2021, I. N. Bolotov, I. V. Vikhrev, E. S. Konopleva, Y. A. Chapurina leg.”Image files (<i>n</i> = 1):- <i>ventral view of the holotype</i> [RMBH Hyd_1184 v.jpg].(2) <b>Paratype RMBH Hyd_1184-p1 </b>(dissected female specimen on a permanent slide with glycerol gelatin mounting medium) labeled “RUSSIA: Primorsky Kray, 45.8451°N, 134.4623°E, Zavitaya River, a tributary of the Bolshaya Ussurka River, Ussuri River basin, host: <i>Middendorffinaia mongolica</i>, 03 August 2021, I. N. Bolotov, I. V. Vikhrev, E. S. Konopleva, Y. A. Chapurina leg.”Image files (<i>n</i> = 1):- <i>ventral view of the paratype</i> [RMBH Hyd_1184-p1 v.jpg].(3) <b>Paratype RMBH Hyd_1184-p2 </b>(dissected female specimen on a permanent slide with glycerol gelatin mounting medium) labeled “RUSSIA: Primorsky Kray, 45.8451°N, 134.4623°E, Zavitaya River, a tributary of the Bolshaya Ussurka River, Ussuri River basin, host: <i>Middendorffinaia mongolica</i>, 03 August 2021, I. N. Bolotov, I. V. Vikhrev, E. S. Konopleva, Y. A. Chapurina leg.”Image files (<i>n</i> = 1):- <i>ventral view of the paratype</i> [RMBH Hyd_1184-p1 v.jpg].(4) <b>Paratype RMBH Hyd_1184-f </b>(dissected female specimen on a permanent slide with glycerol gelatin mounting medium) labeled “RUSSIA: Primorsky Kray, 45.8451°N, 134.4623°E, Zavitaya River, a tributary of the Bolshaya Ussurka River, Ussuri River basin, host: <i>Middendorffinaia mongolica</i>, 03 August 2021, I. N. Bolotov, I. V. Vikhrev, E. S. Konopleva, Y. A. Chapurina leg.”Image files (<i>n</i> = 1):- <i>ventral view of the paratype</i> [RMBH Hyd_1184-f v.jpg].(5) <b>Paratype RMBH Hyd_1184-f </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd_1184-f d 2.tif];- <i>ventral view of the paratype</i> [RMBH Hyd_1184-f v 2.tif].(6) <b>Paratype RMBH Hyd_1184-d </b>(dissected deytonymph specimen on a permanent slide with glycerol gelatin mounting medium) labeled “RUSSIA: Primorsky Kray, 45.8451°N, 134.4623°E, Zavitaya River, a tributary of the Bolshaya Ussurka River, Ussuri River basin, host: <i>Middendorffinaia mongolica</i>, 03 August 2021, I. N. Bolotov, I. V. Vikhrev, E. S. Konopleva, Y. A. Chapurina leg.”Image files (<i>n</i> = 1):- <i>ventral view of the holotype</i> [RMBH Hyd_1184-d v.jpg].(7) <b>Paratype RMBH Hyd_1184-m </b>(dissected male specimen on a permanent slide with glycerol gelatin mounting medium) labeled “RUSSIA: Primorsky Kray, 45.8451°N, 134.4623°E, Zavitaya River, a tributary of the Bolshaya Ussurka River, Ussuri River basin, host: <i>Middendorffinaia mongolica</i>, 03 August 2021, I. N. Bolotov, I. V. Vikhrev, E. S. Konopleva, Y. A. Chapurina leg.”Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd_1184-m d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd_1184-m v.jpg].(8) <b>Paratype RMBH Hyd_1184-m </b>(intact male specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd_1184-m d 2.tif];- <i>ventral view of the paratype</i> [RMBH Hyd_1184-m v 2.tif].(9) <b>Paratype RMBH Hyd 1184_4-DNA (paratype) </b>(specimen was photographed and completely used for DNA extraction) from the type locality.Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd 1184_4-DNA (paratype) d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd 1184_4-DNA (paratype) v.tif].(10) <b>Paratype RMBH Hyd 1184_5-DNA (paratype) </b>(specimen was photographed and completely used for DNA extraction) from the type locality.Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd 1184_5-DNA (paratype) d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd 1184_5-DNA (paratype) v.tif].(11) <b>Paratype RMBH Hyd1184_1 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_1 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_1 v.tif].(12) <b>Paratype RMBH Hyd1184_2 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_2 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_2 v.tif].(13) <b>Paratype RMBH Hyd1184_3 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 3):- <i>genital field of the paratype</i> [RMBH Hyd1184_3 gf.tif];- <i>dorsal view of the paratype</i> [RMBH Hyd1184_3 d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_3 v.tif].(14) <b>Paratype RMBH Hyd1184_4 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 3):- <i>genital field of the paratype</i> [RMBH Hyd1184_4 gf.tif];- <i>dorsal view of the paratype</i> [RMBH Hyd1184_4 d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_4 v.tif].(15) <b>Paratype RMBH Hyd1184_5 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 3):- <i>genital field of the paratype</i> [RMBH Hyd1184_5 gf.tif];- <i>dorsal view of the paratype</i> [RMBH Hyd1184_5 d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_5 v.tif].(16) <b>Paratype RMBH Hyd1184_6 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_6 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_6 v.tif].(17) <b>Paratype RMBH Hyd1184_7 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_7 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_7 v.tif].(18) <b>Paratype RMBH Hyd1184_8 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_8 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_8 v.tif].(19) <b>Paratype RMBH Hyd1184_9 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_9 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_9 v.tif].(20) <b>Paratype RMBH Hyd1184_10 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_10 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_10 v.tif].(21) <b>Paratype RMBH Hyd1184_11 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>genital field of the paratype</i> [RMBH Hyd1184_11 gf.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_11 v.tif].(22) <b>Paratype RMBH Hyd1184_12 </b>(intact female specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd1184_12 d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_12 v.tif].(23) <b>Paratype RMBH Hyd1184_m2 </b>(intact male specimen; fixed in 96 % ethanol) from the type locality.Image files (<i>n</i> = 2):- <i>dorsal view of the paratype</i> [RMBH Hyd1184_m2 d.tif];- <i>ventral view of the paratype</i> [RMBH Hyd1184_m2 v.tif].<br><b>HIGH-RESOLUTION FIGURE FILES:</b><b>Figure 1.</b> Distribution map of <i>Najadicola</i> species. The stars indicate type localities, and the circles indicate non-type occurrences. The point-occurrence records of <i>N. ingens</i> and <i>N. loeiensis</i> were digitized from published sources (Vidrine and Lieux 1980; Vidrine 1996; Chapurina et al. 2019; see Table S1 for detail).<b>Figure 2.</b> Habitus and the type locality of <i>Najadicola tuzovskiji</i> <b>sp. nov.</b><b> </b>(<b>A</b>) Antero-dorsal and (<b>B</b>) ventral view of a live female paratype in a dissected host mussel, <i>Middendorffinaia mongolica</i>. The approximate length of the specimen is 4 mm. (<b>C</b>) Type locality: Zavitaya River, 45.8451°N, 134.4623°E, a tributary of the Bolshaya Ussurka River, Ussuri River basin, Amur River drainage, Primorye, Russian Far East, 3 August 2021 (Photos: Ilya Vikhrev).<b>Figure 3.</b> Maximum likelihood phylogeny of the superfamily Hygrobatoidea<i> </i>based on partial sequences of one mitochondrial and two nuclear genes (<i>COI</i> + <i>18S rRNA</i> + <i>28S rRNA</i>). Numbers near nodes are bootstrap support values. Scale bar indicates the branch lengths. The family-level assignment is highlighted by different colors. The new species’ name is red.<b>Figure 4.</b> General habitus of <i>Najadicola tuzovskiji</i> <b>sp. nov.</b><i> </i>(paratype male Hyd1184-m): (<b>A</b>) dorsal view of idiosoma; (<b>B</b>) ventral view of idiosoma; (<b>C</b>) chelicerae. Scale bars = 200 μm (A, B) and 20 μm (C) (Photos and drawings: Irina A. Kuznetsova).<b>Figure 5.</b><i> </i>Morphological features of <i>Najadicola tuzovskiji</i> <b>sp. nov.</b>: (<b>A</b>) coxal plates and (<b>B</b>) genital field of a paratype female (Hyd1184-p1); (<b>C</b>) coxal plates and (<b>D</b>) genital field of the holotype male (Hyd1184); and (<b>E</b>) ejaculatory complex of a paratype male (Hyd1184-m). Scale bars = 100 μm (A-D) and 20 μm (E) (Drawings: Irina A. Kuznetsova).<b>Figure 6.</b><i> </i>Pedipalps of<i> Najadicola tuzovskiji</i> <b>sp. nov.</b>: (<b>A</b>) paratype female; (<b>B</b>) holotype male. Scale bars = 100 μm (Drawings: Irina A. Kuznetsova).<b>Figure 7.</b> Legs of <i>Najadicola tuzovskiji</i> <b>sp. nov.</b>: (<b>A</b>) paratype female; (<b>B</b>) holotype male. Scale bars = 100 μm (Drawings: Irina A. Kuznetsova).<br><b>References</b>Chapurina, Y. E., Vikhrev, I. V., Kondakov, A. V. & Tanmuangpak, K. (2019) A new <i>Najadicola </i>species (Acari: Hydrachnidia: Pionidae) from Asia. <i>Ecologica Montenegrina</i>, 24, 32–37. https://doi.org/10.37828/em.2019.24.6Kuznetsova, I. A., Vikhrev, I. V., Konopleva, E. S., Kogut, Y. E., Gofarov, M. Y., Kondakov, A. V. & Bolotov, I. N. (2025) A new giant mussel-associated mite from Northeast Asia, with an assessment of the phylogenetic position of the genus Najadicola Piersig, 1897 (Acari: Hydrachnidia: Pionidae). <i>Ecologica Montenegrina</i>, 92, 36-53. https://doi.org/10.37828/em.2025.92.4Vidrine, M. F. & Lieux, M. H. (1980) Najadicolinae, an ancient and distinctive clade. <i>The Association of Southeastern Biologists Bulletin</i>, 27(2), 68.Vidrine, M. F. (1996) <i>North American Najadicola and Unionicola: Diagnoses and Distributions</i>. Gail Q. Vidrine Collectibles, Eunice, 355 pp.