cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/nq9w6-rxc22
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-132/rawdata/</p>9082_box1_No1_54.mrc,  Tilt Series (Pixel Size 0.2612 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-132/rawdata/9082_box1_No1_54.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_box1_No1_54_full.rec,  Reconstruction (Pixel Size 1.0448 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-132/3dimage_110339/9082_box1_No1_54_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xc0w1-ypb75
title: JV9298

description: DotF-sfGFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2019-10-14</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> PNCC Krios 3</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential/Early stationary</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper. Vitrobot 22 deg C, 100% humidity, 4uL of 2X concentrated culture, blot force 6, blot time 3 sec, drain time 1 sec, wait time 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-10-14-8/rawdata/</p>JV9298_113_D4.mrc,  Tilt Series (Pixel Size 0.1323 nm), 14.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-10-14-8/rawdata/JV9298_113_D4.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_113_D4_part37_11.rec,  Reconstruction (Pixel Size 1.0584 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-10-14-8/3dimage_109425/JV9298_113_D4_part37_11.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4ec1g-was11
title: Legionella_delta-lspHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-35/rawdata/</p>DG7_20190516_tomo0063.mrc,  Tilt Series (Pixel Size 0.26 nm), 882.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-35/rawdata/DG7_20190516_tomo0063.mrc"
        > <i class="download icon"></i> Download </a></p>

        DG7_20190516_tomo0063_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-35/3dimage_102694/DG7_20190516_tomo0063_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.pqbfsh
title: Occurrence Download

description: A dataset containing 164 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "GadmGid is CHN.28_1",
 "TaxonKey is Neotrinia splendens"
 ]
}

 The dataset includes 164 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002489-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/n4p7r-3gw76
title: MEF cells

description: Date of collection wrong in the tilt series ID. Should be Jan 3, 2019
description: <strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-41/rawdata/</p>TS15.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-41/rawdata/TS15.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS15_bin4_SIRT_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 748.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-41/3dimage_101857/TS15_bin4_SIRT_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5f62k-17r62
title: Xanthomonas WT

description: Xac WT pBRaφ pBRR
description: <strong>Tilt Series Date:</strong> 2019-12-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Carvalho and Farah lab sent the strain. Debnath Ghosal collected data</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in 2xTY with apt antibiotics, freeze cells next day after about 14 hour when they are in stationary phase.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 5 sec. ** Use 7 sec for next sessions.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-40/rawdata/</p>Xac_No1_TS64.mrc,  Tilt Series (Pixel Size 0.353 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-40/rawdata/Xac_No1_TS64.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_No1_TS64_full.rec,  Reconstruction (Pixel Size 1.412 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-06-40/3dimage_112417/Xac_No1_TS64_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/esmcv-r0s43
title: Legionella IcmQ71::TevS 42C_On

description: Legionella IcmQ71::TevS 42C_On (HS004 E)
description: <strong>Tilt Series Date:</strong> 2019-05-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Howard Shuman (University of Chicago). Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-75/rawdata/</p>HS004E-area9-tomo0006.mrc,  Tilt Series (Pixel Size 0.26 nm), 996.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-75/rawdata/HS004E-area9-tomo0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        HS004E-area9-tomo0006_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-75/3dimage_103780/HS004E-area9-tomo0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bt941-n4s76
title: B. diazoefficiens, hopanoids

description: WT strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-32/rawdata/</p>WT_LG_64step2_21.mrc,  Tilt Series (Pixel Size 2.678 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-32/rawdata/WT_LG_64step2_21.mrc"
        > <i class="download icon"></i> Download </a></p>

        WT_LG_64step2_21.rec,  Reconstruction (Pixel Size 10.712 nm), 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-32/3dimage_108310/WT_LG_64step2_21.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/120tk-r3j44
title: ec9-3_sq1_cell3

description: IT 1.2s
description: <strong>Tilt Series Date:</strong> 2019-01-18</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 1094</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 68.0 eV/Å²,  defocus: -2.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Method 1 used (spotted drops of culture on agar plate ON and then resuspended culture in 0.5mL LB). LB is supplemented with 0.2g/L cellulasePG was added 1:30 before freezing</p>
<strong>Sample Preparation:</strong> Culture diluted 2x - Broad 100% 22C - backblotted 6s, glare seen at 3-4s</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-01-18-5/rawdata/</p>ec9-3_sq1_cell3.mrc,  Tilt Series (Pixel Size 0.54 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-01-18-5/rawdata/ec9-3_sq1_cell3.mrc"
        > <i class="download icon"></i> Download </a></p>

        ec9-3_sq1_cell3_full.rec,  Reconstruction (Pixel Size 2.16 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-01-18-5/3dimage_100772/ec9-3_sq1_cell3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bt078-3tn95
title: RalF-Ub V76

description: Lp02 pCyaA::RalF-Ub V76 free from U937
description: <strong>Tilt Series Date:</strong> 2020-07-31</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, 1-2 washes, fibronectin/vitronectin/no matrix. Legionella: 2.5 O.D, 3h after 200mM  IPTG induction, at O.D. 3</p>
<strong>Sample Preparation:</strong> 200mesh Gold quantifoil,  10nm gold, 37deg C, 80-90% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-43/rawdata/</p>RalFUbV76_177.mrc,  Tilt Series (Pixel Size 0.265 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-43/rawdata/RalFUbV76_177.mrc"
        > <i class="download icon"></i> Download </a></p>

        RalFUbV76_177_full.rec,  Reconstruction (Pixel Size 1.06 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-43/3dimage_113261/RalFUbV76_177_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y61vx-gyc49
title: Legionella IcmQ71::TevS 42C_On

description: Legionella IcmQ71::TevS 42C_On (HS004 E)
description: <strong>Tilt Series Date:</strong> 2019-05-31</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Howard Shuman (University of Chicago). Data collected by Debnath Ghosal and Yuxi Liu.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-34/rawdata/</p>HS004E-area2-tomo0003.mrc,  Tilt Series (Pixel Size 0.26 nm), 996.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-34/rawdata/HS004E-area2-tomo0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        HS004E-area2-tomo0003_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-31-34/3dimage_103692/HS004E-area2-tomo0003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vhthx-89y24
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-187/rawdata/</p>9082_box1_No4_54.mrc,  Tilt Series (Pixel Size 0.2612 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-187/rawdata/9082_box1_No4_54.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_box1_No4_54_full.rec,  Reconstruction (Pixel Size 1.0448 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-187/3dimage_110441/9082_box1_No4_54_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3j1g4-m4h09
title: RalF-Ub V76

description: Lp02 pCyaA::RalF-Ub V76 free from U937
description: <strong>Tilt Series Date:</strong> 2020-07-31</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Stephen Carter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, 1-2 washes, fibronectin/vitronectin/no matrix. Legionella: 2.5 O.D, 3h after 200mM  IPTG induction, at O.D. 3</p>
<strong>Sample Preparation:</strong> 200mesh Gold quantifoil,  10nm gold, 37deg C, 80-90% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-07-31-101/rawdata/</p>RalFUbV76_258.mrc,  Tilt Series (Pixel Size 0.265 nm), 1.7 GB  
        
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        RalFUbV76_258_part37_5.rec,  Reconstruction (Pixel Size 1.06 nm), 516.1 MB  
        
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