DOI: 10.5061/dryad.kprr4xh1f
title: A morphological trait involved in reproductive isolation between <em>Drosophila</em> sister species is sensitive to temperature

description: Male genitalia are usually extremely divergent between closely related
 species, but relatively constant within one species. Here, we examine the
 effect of temperature on the shape of the ventral branches, a male genital
 structure involved in reproductive isolation, in the sister species
 Drosophila santomea and D. yakuba. We designed a semi-automatic
 measurement machine learning pipeline that can reliably identify
 curvatures and landmarks based on manually digitized contours of the
 ventral branches. With this method, we observed that temperature does not
 affect ventral branches in D. yakuba, but that in D. santomea ventral
 branches tend to morph into a D. yakuba-like shape at lower temperatures.
 We found that male genitalia structures involved in reproductive isolation
 can be relatively variable within one species and can resemble the shape
 of closely related species’ genitalia through plasticity to temperature.
 Our results suggest that reproductive isolation mechanisms may be
 dependent on the environmental context.
description: # A morphological trait involved in reproductive isolation between
 *Drosophila* sister species is sensitive to temperature Dataset DOI:
 [10.5061/dryad.kprr4xh1f](https://doi.org/10.5061/dryad.kprr4xh1f)
 associated article: Peluffo AE, Hamdani M, Vargas-Valderrama A, David JR,
 Mallard F, Graner F, Courtier-Orgogozo V. (2021) A morphological trait
 involved in reproductive isolation between Drosophila sister species is
 sensitive to temperature. Ecology and Evolution 11(12), 7492-7506. doi:
 [10.1002/ece3.7580](doi.org/10.1002/ece3.7580) ## Description of the data
 and file structure This readme file was generated on 2025-11-19 by
 Virginie COURTIER-ORGOGOZO GENERAL INFORMATION Title of Dataset: Dataset
 for "A morphological trait involved in reproductive isolation between
 Drosophila sister species is sensitive to temperature"
 Author/Principal Investigator Information\ Name: Courtier-Orgogozo\
 Institution: CNRS, Institut Jacques Monod, Univ de Paris\ Address: Paris,
 France\ Email: [virginie.courtier@ijm.fr](mailto:virginie.courtier@ijm.fr)
 First Author Information\ Name: Peluffo\ ORCID:\ Institution: CNRS,
 Institut Jacques Monod, Univ de Paris\ Address: Paris\ Email:
 [alexandre.peluffo@gmail.com](mailto:alexandre.peluffo@gmail.com) \- Date
 of data collection: 2020 \- Information about funding sources that
 supported the collection of the data: European Research Council under the
 European Community’s Seventh Framework Program (FP7/2007-2013 Grant
 Agreement no. 337579) ; labex “Who am I?” (ANR-11-LABX-0071) and the
 Université de Paris IdEx (ANR-18-IDEX-0001) funded by the French
 government through grant no. ANR-11-IDEX-0005-02. ### Files and variables
 contours.zip\ The contour folder contains all contour measures obtained
 via the custom ImageJ pluggin. Each subfolder is one isofemale line at one
 specific temperature following the nomenclature of Table 1 (list of
 isofemale lines) and each .txt file is a tab separated file where each
 line is the x (first column) and y (second column) coordinate of the pixel
 under the contour. pictures.zip\ The picture folder contains the
 corresponding pictures for the contour files using the same nomenclature.
 parametrization.zip\ This folder contains the pictures, contour and
 hand-positionned landmarks (.tps file) for D. santomea strain 1563 and D.
 yakuba Raphia. For D. yakuba Raphia, other contours can be found in the
 countour folder. Total number of individuals in "pictures" and
 "contour"\ 684 Pictures + contours\ 614 After removing 70 not
 dissected or mounted correctly\ 584 For statistical analyses after
 removing 30 which have ill landmarks Total number of individuals in
 parametrization\ 31 D.san\ 30 D.yak ## Code/software
 Peluffoetal_plasticityVB_analyses_v1.R\ This R script contains all
 statistical analyses on the spine-thrust measures.
 Peluffoetal_plasticityVB_autodetect_v1.R\ This R script contains the
 preprocessing, the automatic detection of landmarks using learned
 parameters and the automatic measure of spine-thrust.
 Peluffoetal_plasticityVB_parametrization_v1.R\ This R script is the
 learning of the automatic detection algorithm parameters.
 Peluffoetal_plasticityVB_supportfunctions_v1.R\ This R script contains
 several R functions used by the autodetect R script.
 Peluffoetal_contourpluginimageJ.java\ This java script is a plugin to
 ImageJ in order to record the coordinates of each pixel along the drawn
 contour on ImageJ. This plugin requests an input directory that contains
 the pictures to be analyzed or sub-directories containing pictures that
 will be successively analyzed. The output is a tab-separated file, for
 each picture (here individuals), with the x (first column) and y (second
 column) coordinates of the points where the contour passes.
 Wait_For_User.java\ This java script plugs to the contour java script and
 triggers a ask-user window before opening the next picture in the stack.
 ## Access information Other publicly accessible locations of the data: *
 None