cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.26356761.v1
title: Rapid identification of Africanized honey bee <i>Apis mellifera</i> (hymenoptera: apidae) using high-resolution melting analysis DNA from honey

description: Genetic characterization of honey bee populations, particularly the identification of Africanized honey bees, has traditionally been based on different molecular methods that involve the amplification and analysis of different regions of the mitochondrial or nuclear genome which often take a long time. We introduce high-resolution melting analysis (HRM) as a novel approach for rapid and cost-effective identification of the African lineage in <i>Apis mellifera</i> populations from mitochondrial DNA extracted from honey. Samples were collected from 111 apiaries in 13 Argentinean provinces between 2012 and 2018. Total DNA was extracted from 10 g honey sediment and subjected to quantitative PCR (qPCR) using specific primers (APIS-F/Afr207R) amplifying a 207 bp product. Positive controls (Africanized/Non-Africanized) were used for mitotype validation and a subset of qPCR products were sequenced to compare different haplotypes. HRM analysis enabled the quick detection of sequence polymorphisms in short amplicons generated by qPCR. Among the analyzed apiaries, 26 located above 35° S in Misiones, La Rioja, Catamarca, Formosa, Corrientes, Córdoba, and Santa Fé exhibited the Africanized mitotypes while the remaining apiaries below this latitude, mainly in Buenos Aires, Rio Negro, Neuquén, and Entre Ríos, showed the European mitotypes. These results align with previous characterizations in the region. HRM analysis demonstrated high sensitivity and effectiveness, requiring minimal DNA input, and reducing costs and sampling efforts. This approach provides a rapid and reliable method for identifying the African lineage, facilitating honey bee breeding programs, conservation efforts, and management strategies in regions with Africanized honey bee populations.
DOI: 10.15468/dl.xt4tq6
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Occurrence evidence, Observation)",
 "TaxonKey is one of (Tenrec ecaudatus)",
 "Country is one of (American Samoa, Australia, Christmas Island, Cocos (Keeling) Islands, Cook Islands, Fiji, French Polynesia, Guam, Heard Island and McDonald Islands, Kiribati, Marshall Islands, Micronesia (Federated States of), Nauru, New Caledonia, New Zealand, Niue, Norfolk Island, Northern Mariana Islands, Palau, Papua New Guinea, Pitcairn, Samoa, Solomon Islands, Tokelau, Tonga, Tuvalu, United States Minor Outlying Islands, Vanuatu, Wallis and Futuna)"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0025587-250802193616735/datasets/export for details.
DOI: 10.6084/m9.figshare.26356761
title: Rapid identification of Africanized honey bee <i>Apis mellifera</i> (hymenoptera: apidae) using high-resolution melting analysis DNA from honey

description: Genetic characterization of honey bee populations, particularly the identification of Africanized honey bees, has traditionally been based on different molecular methods that involve the amplification and analysis of different regions of the mitochondrial or nuclear genome which often take a long time. We introduce high-resolution melting analysis (HRM) as a novel approach for rapid and cost-effective identification of the African lineage in <i>Apis mellifera</i> populations from mitochondrial DNA extracted from honey. Samples were collected from 111 apiaries in 13 Argentinean provinces between 2012 and 2018. Total DNA was extracted from 10 g honey sediment and subjected to quantitative PCR (qPCR) using specific primers (APIS-F/Afr207R) amplifying a 207 bp product. Positive controls (Africanized/Non-Africanized) were used for mitotype validation and a subset of qPCR products were sequenced to compare different haplotypes. HRM analysis enabled the quick detection of sequence polymorphisms in short amplicons generated by qPCR. Among the analyzed apiaries, 26 located above 35° S in Misiones, La Rioja, Catamarca, Formosa, Corrientes, Córdoba, and Santa Fé exhibited the Africanized mitotypes while the remaining apiaries below this latitude, mainly in Buenos Aires, Rio Negro, Neuquén, and Entre Ríos, showed the European mitotypes. These results align with previous characterizations in the region. HRM analysis demonstrated high sensitivity and effectiveness, requiring minimal DNA input, and reducing costs and sampling efforts. This approach provides a rapid and reliable method for identifying the African lineage, facilitating honey bee breeding programs, conservation efforts, and management strategies in regions with Africanized honey bee populations.
DOI: 10.15468/dl.tncqct
title: Occurrence Download

description: A dataset containing 439 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Occurrence evidence, Observation)",
 "TaxonKey is one of (Tenrec ecaudatus)",
 "Country is one of (Algeria, Angola, Benin, Botswana, British Indian Ocean Territory, Burkina Faso, Burundi, Cabo Verde, Cameroon, Central African Republic, Chad, Comoros, Congo, Congo, Democratic Republic of the, Côte d’Ivoire, Djibouti, Egypt, Equatorial Guinea, Eritrea, Eswatini, Ethiopia, French Southern Territories, Gabon, Gambia, Ghana, Guinea, Guinea-Bissau, Kenya, Lesotho, Liberia, Libya, Madagascar, Malawi, Mali, Mauritania, Mauritius, Mayotte, Morocco, Mozambique, Namibia, Niger, Nigeria, Réunion, Rwanda, Saint Helena, Ascension and Tristan da Cunha, Sao Tome and Principe, Senegal, Seychelles, Sierra Leone, Somalia, South Africa, South Sudan, Sudan, Tanzania, United Republic of, Togo, Tunisia, Uganda, Western Sahara, Zambia, Zimbabwe)"
 ]
}

 The dataset includes 439 records from 22 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0025590-250802193616735/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.17632/t99pbfp6j2
title: The mitigation of food insecurity in Africa through climate-smart agriculture

description: The mitigation of food insecurity in Africa through climate-smart agriculture
DOI: 10.6084/m9.figshare.26509345
title: Flight activity behavior of stingless bee (Apidae: Meliponini) in an urban park outside their natural range: the influence of hive temperature

description: In South America, the movement of stingless bee colonies outside their natural location is a common practice and, in many cases, meliponiculture activities are conducted in urban or semi-urban areas. In this study, we used a temperature/relative humidity probe inside the hives to assess indicators of hive dynamics to explore the adaptation of <i>Scaptotrigona jujuyensis</i> colonies located in an urban park outside the species’ natural range. The temperature inside the hives was negatively correlated with environmental temperature and its range was narrower than in the empty box (control). These results suggest that the colonies regulate the internal temperature and form a microenvironment, with average values ranging between 29 and 21 °C in the brood chamber, and between 27 and 19 °C in the storage chamber. The number of bees involved in the three flight activities studied varied with the time of the day, through the season and colony. Nevertheless, between 10:00 and 15:00 h, all colonies showed high and continuous activity. The pattern of activity curves of <i>S. jujuyensis</i> tends to be similar to other <i>Scaptotrigona</i>. Finally, we fit Generalized Linear Models to assess the effect of abiotic variables on the flight activity of <i>S. jujuyensis</i> through a negative binomial model. Our results show that the temperature of the brood chamber, relative humidity and colony size best predict the collection of pollen and removal pellets into the hive. These results contribute to the management and conservation of this native species in a new scenario of geographic distribution.
DOI: 10.6084/m9.figshare.26509345.v1
title: Flight activity behavior of stingless bee (Apidae: Meliponini) in an urban park outside their natural range: the influence of hive temperature

description: In South America, the movement of stingless bee colonies outside their natural location is a common practice and, in many cases, meliponiculture activities are conducted in urban or semi-urban areas. In this study, we used a temperature/relative humidity probe inside the hives to assess indicators of hive dynamics to explore the adaptation of <i>Scaptotrigona jujuyensis</i> colonies located in an urban park outside the species’ natural range. The temperature inside the hives was negatively correlated with environmental temperature and its range was narrower than in the empty box (control). These results suggest that the colonies regulate the internal temperature and form a microenvironment, with average values ranging between 29 and 21 °C in the brood chamber, and between 27 and 19 °C in the storage chamber. The number of bees involved in the three flight activities studied varied with the time of the day, through the season and colony. Nevertheless, between 10:00 and 15:00 h, all colonies showed high and continuous activity. The pattern of activity curves of <i>S. jujuyensis</i> tends to be similar to other <i>Scaptotrigona</i>. Finally, we fit Generalized Linear Models to assess the effect of abiotic variables on the flight activity of <i>S. jujuyensis</i> through a negative binomial model. Our results show that the temperature of the brood chamber, relative humidity and colony size best predict the collection of pollen and removal pellets into the hive. These results contribute to the management and conservation of this native species in a new scenario of geographic distribution.
DOI: 10.6084/m9.figshare.27194256
title: Molecular detection and phylogenetic analysis of six bee viruses from <i>Varroa destructor</i> in Korea

description: In the beekeeping industry, the control of <i>Varroa destructor</i> and viruses is a crucial factor in maintaining the health of the western honey bee <i>Apis mellifera L.</i> Recently, in Korea, beekeepers suffered for high colony losses during the overwintering, and the presence of <i>V. destructor</i> and viruses has been considered an important factor for honey bee health. In this study, we investigated the presence of six honey bee viruses [deformed wing virus (DWV), sacbrood virus (SBV), Kashmir bee virus (KBV), Israeli acute paralysis virus (IAPV), chronic bee paralysis virus (CBPV), and black queen cell virus (BQCV)] in <i>V. destructor</i> in Korea. <i>V. destructor</i> was collected from 46 apiaries in nine provinces in Korea, and viruses were detected using reverse transcription–polymerase chain reaction. DWV was most predominantly detected at 87% of apiaries, followed by IAPV, SBV, KBV, BQCV, and CBPV that were detected in 65.2%, 17.4%, 13%, 8.7%, and 6.5% of apiaries in Korea. Phylogenetic analysis revealed that five viruses (IAPV, SBV, BQCV, and CBPV) were grouped into clades of viruses originating from China, Russia, and Australia, but KBV showed no distinct clades of countries. This study confirmed the presence of various types of bee viruses in <i>V. destructor</i> in Korea, indicating that domestic honey bees are exposed to multiple viruses <i>via V. destructor</i>.
DOI: 10.6084/m9.figshare.27194256.v1
title: Molecular detection and phylogenetic analysis of six bee viruses from <i>Varroa destructor</i> in Korea

description: In the beekeeping industry, the control of <i>Varroa destructor</i> and viruses is a crucial factor in maintaining the health of the western honey bee <i>Apis mellifera L.</i> Recently, in Korea, beekeepers suffered for high colony losses during the overwintering, and the presence of <i>V. destructor</i> and viruses has been considered an important factor for honey bee health. In this study, we investigated the presence of six honey bee viruses [deformed wing virus (DWV), sacbrood virus (SBV), Kashmir bee virus (KBV), Israeli acute paralysis virus (IAPV), chronic bee paralysis virus (CBPV), and black queen cell virus (BQCV)] in <i>V. destructor</i> in Korea. <i>V. destructor</i> was collected from 46 apiaries in nine provinces in Korea, and viruses were detected using reverse transcription–polymerase chain reaction. DWV was most predominantly detected at 87% of apiaries, followed by IAPV, SBV, KBV, BQCV, and CBPV that were detected in 65.2%, 17.4%, 13%, 8.7%, and 6.5% of apiaries in Korea. Phylogenetic analysis revealed that five viruses (IAPV, SBV, BQCV, and CBPV) were grouped into clades of viruses originating from China, Russia, and Australia, but KBV showed no distinct clades of countries. This study confirmed the presence of various types of bee viruses in <i>V. destructor</i> in Korea, indicating that domestic honey bees are exposed to multiple viruses <i>via V. destructor</i>.
DOI: 10.17632/t99pbfp6j2.1
title: The mitigation of food insecurity in Africa through climate-smart agriculture

description: The mitigation of food insecurity in Africa through climate-smart agriculture
DOI: 10.6084/m9.figshare.29085859
title: Note: Cytonuclear patterns of a honey bee population from the Azores show a stable population at the nuclear but not at the mitochondrial DNA level

description: The Azores archipelago has been the stage for multiple introductions of <i>Apis mellifera</i> from varying origins, which have led to widespread admixture and the existence of phenotypically and genotypically heterogeneous populations. This is evident on the São Miguel Island, where the historically introduced black phenotype of <i>A. m. iberiensis</i> (lineage M) co-exists with the contemporaneously introduced yellow phenotype of C-lineage ancestry. Interestingly, the cytonuclear markers used herein revealed that C-lineage ancestry is residual at the nuclear level for both the black (5.82 ± 1.66%) and yellow (5.91 ± 1.85%) phenotypes, although this is more pronounced at the mitochondrial level (27.27% for black and 14.74% for yellow). While the C-lineage contribution has remained stable at the nuclear level for over 20 years, there has been a recent decrease in the proportion of C-derived mitotypes.
DOI: 10.6084/m9.figshare.29085859.v1
title: Note: Cytonuclear patterns of a honey bee population from the Azores show a stable population at the nuclear but not at the mitochondrial DNA level

description: The Azores archipelago has been the stage for multiple introductions of <i>Apis mellifera</i> from varying origins, which have led to widespread admixture and the existence of phenotypically and genotypically heterogeneous populations. This is evident on the São Miguel Island, where the historically introduced black phenotype of <i>A. m. iberiensis</i> (lineage M) co-exists with the contemporaneously introduced yellow phenotype of C-lineage ancestry. Interestingly, the cytonuclear markers used herein revealed that C-lineage ancestry is residual at the nuclear level for both the black (5.82 ± 1.66%) and yellow (5.91 ± 1.85%) phenotypes, although this is more pronounced at the mitochondrial level (27.27% for black and 14.74% for yellow). While the C-lineage contribution has remained stable at the nuclear level for over 20 years, there has been a recent decrease in the proportion of C-derived mitotypes.
DOI: 10.6084/m9.figshare.26356833
title: Effective control of <i>Varroa destructor</i> mites with <i>Juniperus excelsa, Juglans regia, Origanum onites, and Mentha spicata</i> leaf extracts: a study of honey bee colonies

description: This study aimed to assess the efficacy of plant leaf extracts from <i>Juniperus excelsa (J. excelsa), Juglans regia (J. regia), Origanum onites (O. onites)</i>, and <i>Mentha spicata (M. spicata)</i> in controlling <i>Varroa destructor (V. destructor)</i> mites, a major cause of honey bee colony loss. This research seeks to identify eco-friendly and sustainable alternatives for pest control in beekeeping practices. Forty honey bee colonies naturally infested with mites were selected. The colonies were divided into five groups, one control group and four treatment groups, which received varying concentrations of the plant leaf extracts. This study was conducted in two phases: determining the optimal dosage and conducting a field study during autumn and spring, during which the mite infestation levels were evaluated using the powdered sugar method. The powdered sugar method revealed that a 25% concentration of plant leaf extract was the most effective. In the autumn treatment, the efficacy rates were 87% for <i>J. regia,</i> 86% for <i>J. excelsa,</i> 83% for <i>O. onites,</i> and 68% for <i>M. spicata</i>. During spring treatment, rates were slightly reduced but remained promising, with 66% for <i>O. onites</i>, 63% for <i>J. regia</i>, 61% for <i>J. excelsa</i>, and 55% for <i>M. spicata.</i> This study highlights that plant leaf extracts offer a viable and eco-friendly alternative to traditional therapies for controlling Varroa in beekeeping when used at the appropriate dosage. This research provides valuable insights into sustainable pest management and the potential for innovative, environmentally friendly pest control solutions based on aromatic plant products.
DOI: 10.6084/m9.figshare.26356833.v1
title: Effective control of <i>Varroa destructor</i> mites with <i>Juniperus excelsa, Juglans regia, Origanum onites, and Mentha spicata</i> leaf extracts: a study of honey bee colonies

description: This study aimed to assess the efficacy of plant leaf extracts from <i>Juniperus excelsa (J. excelsa), Juglans regia (J. regia), Origanum onites (O. onites)</i>, and <i>Mentha spicata (M. spicata)</i> in controlling <i>Varroa destructor (V. destructor)</i> mites, a major cause of honey bee colony loss. This research seeks to identify eco-friendly and sustainable alternatives for pest control in beekeeping practices. Forty honey bee colonies naturally infested with mites were selected. The colonies were divided into five groups, one control group and four treatment groups, which received varying concentrations of the plant leaf extracts. This study was conducted in two phases: determining the optimal dosage and conducting a field study during autumn and spring, during which the mite infestation levels were evaluated using the powdered sugar method. The powdered sugar method revealed that a 25% concentration of plant leaf extract was the most effective. In the autumn treatment, the efficacy rates were 87% for <i>J. regia,</i> 86% for <i>J. excelsa,</i> 83% for <i>O. onites,</i> and 68% for <i>M. spicata</i>. During spring treatment, rates were slightly reduced but remained promising, with 66% for <i>O. onites</i>, 63% for <i>J. regia</i>, 61% for <i>J. excelsa</i>, and 55% for <i>M. spicata.</i> This study highlights that plant leaf extracts offer a viable and eco-friendly alternative to traditional therapies for controlling Varroa in beekeeping when used at the appropriate dosage. This research provides valuable insights into sustainable pest management and the potential for innovative, environmentally friendly pest control solutions based on aromatic plant products.
DOI: 10.6084/m9.figshare.26013910
title: Advice on how to start beekeeping, memories with bees and the uses of honey: results of an online questionnaire with European beekeepers

description: Beekeeping is a demanding activity that requires both particular human qualities from those who practise it and an environment that is favourable to bees. This is why the interviewed beekeepers advise to take time to think before starting to keep the bees, to find a mentor, and to always have the desire to get to know the bees even if this is not enough for success. In Northern and Western Europe, beekeepers consider patience and calmness as essential qualities to be a “<i>good beekeeper</i>”, while in Southern Europe, passion for bees is the main driver of success. The reasons for abandonment or failure also include human and environmental factors. To better understand the relationship between beekeepers and their bees, interviewees were encouraged to share their best memories. With years of experience, contemplative memories fade into memorable situations in which bee practices are described, and then in turn, they fade into moments of sociability. Honey harvesting plays an important role. Productive considerations are more expressed in Southern Europe, where it is an exceptional harvest that marks on people’s minds, more than the first honey harvest. The strong connection to their bees and the territory they live in is also expressed in the choice of their favourite honey. Many beekeepers mentioned the local honey production, which they are proud to produce with their own bees, even though they sometimes became criticized for a too high price for this local product.
DOI: 10.6084/m9.figshare.26013910.v1
title: Advice on how to start beekeeping, memories with bees and the uses of honey: results of an online questionnaire with European beekeepers

description: Beekeeping is a demanding activity that requires both particular human qualities from those who practise it and an environment that is favourable to bees. This is why the interviewed beekeepers advise to take time to think before starting to keep the bees, to find a mentor, and to always have the desire to get to know the bees even if this is not enough for success. In Northern and Western Europe, beekeepers consider patience and calmness as essential qualities to be a “<i>good beekeeper</i>”, while in Southern Europe, passion for bees is the main driver of success. The reasons for abandonment or failure also include human and environmental factors. To better understand the relationship between beekeepers and their bees, interviewees were encouraged to share their best memories. With years of experience, contemplative memories fade into memorable situations in which bee practices are described, and then in turn, they fade into moments of sociability. Honey harvesting plays an important role. Productive considerations are more expressed in Southern Europe, where it is an exceptional harvest that marks on people’s minds, more than the first honey harvest. The strong connection to their bees and the territory they live in is also expressed in the choice of their favourite honey. Many beekeepers mentioned the local honey production, which they are proud to produce with their own bees, even though they sometimes became criticized for a too high price for this local product.