cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.26661340.v1
title: Immunogenicity of trivalent DNA vaccine candidate encapsulated in Chitosan-TPP nanoparticles against EV-A71 and CV-A16

description: <b>Aim:</b> To develop a trivalent DNA vaccine candidate encapsulated in Chitosan-TPP nanoparticles against hand foot and mouth disease (HFMD) and assess its immunogenicity in mice. <b>Materials & methods:</b> Trivalent plasmid carrying the VP1 and VP2 genes of EV-A71, VP1 gene of CV-A16 was encapsulated in Chitosan-TPP nanoparticles through ionic gelation. <i>In vitro</i> characterization and <i>in vivo</i> immunization studies of the CS-TPP-NPs (pIRES-VP121) were performed. <b>Results:</b> Mice administered with CS-TPP NPs (pIRES-VP121) intramuscularly were observed to have the highest IFN-γ response. Sera from mice immunized with the naked pDNA and CS-TPP-NPs (pIRES-VP121) demonstrated good viral clearance against wild-type EV-A71 and CV-A16 in RD cells. <b>Conclusion:</b> CS-TPP-NPs (pIRES-VP121) could serve as a prototype for future development of multivalent HFMD DNA vaccine candidates. Currently, there are no US-FDA approved vaccine against HFMD. China-FDA approved monovalent HFMD inactivated vaccines were unable to provide cross protection against other enterovirus serotypes. We have developed a trivalent plasmid vaccine candidate, pIRES-VP121 which encodes the VP1 and VP2 genes of EV-A71 and the VP1 gene of CV-A16. We successfully synthesized Chitosan TPP nanoparticles encapsulating DNA Encapsulation of plasmid vaccine candidate pIRES-VP121 through ionic gelation, yielding highly monodisperse nanoparticles with CS-TPP-NPs (pIRES-VP121) with &gt;70% encapsulation efficiency. Encapsulation of plasmid vaccine candidate pIRES-VP121 through ionic gelation successfully formulated sub-200 nm, spherical shaped, mildly cationic (+10 mV) and highly monodisperse CS-TPP-NPs (pIRES-VP121) with &gt;70% encapsulation efficiency. Chitosan NPs encapsulations improved T cell immunogenicity resulting in higher IFN-γ expressing CD4<sup>+</sup>/CD8<sup>+</sup> T cells population and IFN-γ secretion compared with mice immunized with naked pIRES-VP121. Enhanced B cell neutralizing efficacy were demonstrated by mice sera (1:16 dilution) immunized with CS-TPP-NPs (pIRES-VP121) against the wild-type EV-A71 B4 and CV-A16/N132 strain enteroviruses in comparison with naked pIRES-VP121 immunization. These findings showed the potential of a trivalent DNA plasmid to serve as a prototype for future development of multivalent DNA vaccine candidate against HFMD.
DOI: 10.57754/fdat.e7jq0-pqy04
title: Self-paced reading study "Chartreux"

description: How does the meaning of linguistic expressions arise? This is the central research question of the SFB 833 'The Construction of Meaning. The Dynamics and Adaptivity of Linguistic Structures.' Focusing on the fact that meaning emerges as a function of time and situation, the SFB's joint research goal is a better understanding of linguistic meaning and its dynamic adaptation to the various factors that impact it.
description: Research carried out in work package A01 of the SFB 833.
DOI: 10.6084/m9.figshare.27074255
title: Decitabine enclosed biotin-zein conjugated nanoparticles: synthesis, characterization, <i>in vitro</i> and <i>in vivo</i> evaluation

description: <b>Aim:</b> This study focuses on biotinylated nanocarriers designed to encapsulate amphiphilic molecules with self-biodegradable properties for enhanced drug delivery. <b>Methods:</b> Biotin-zein conjugated nanoparticles were synthesized and tested in C6 cell lines to evaluate their viability and cellular uptake. Optimization was achieved using a a central composite design. The nanoparticles underwent thermogravimetric analysis, and their pharmacokinetics and biodistribution were also studied. <b>Results:</b> The optimized nanoparticles displayed 96.31% drug encapsulation efficiency, a particle size of 95.29 nm and a zeta potential of -17.7 mV. These nanoparticles showed increased cytotoxicity and improved cellular uptake compared with free drugs. Thermogravimetric analysis revealed that the drug-loaded nanocarriers provided better protection against drug degradation. Pharmacokinetic and biodistribution studies indicated that the formulation had an extended brain residence time, highlighting its effectiveness. <b>Conclusion:</b> The biotin-zein conjugated nanoparticles developed in this study offer a promising nano-vehicle for <i>in vivo</i> biodistribution and pharmacokinetic applications. Their high drug encapsulation efficiency, stability and extended brain residence time suggest they are effective for targeted drug delivery and therapeutic uses. A stable biotin and zein conjugate were formed via ethyl carbodiimide and N-hydroxy succinimide coupling to enhance site-directed delivery. Prepared polymeric conjugates were evaluated for its extent of conjugation, and secondary structural alteration revealed stability of conjugate. The biotin-zein conjugate exhibited a flexible backbone, and better encapsulation of hydrophilic molecules with a sustained release profile. Biotin-zein conjugation improved the loading capacity, and amphiphilic nature and aided the cytotoxic potential of the drug. The conjugated nanocarriers revealed the sustained release profile with higher cytotoxic potential compared with free drug along with enhanced cellular entrapment. The prepared nanoformulation revealed its safety via the nasal mucociliary canal. The formulation suggests higher residence time is observed via the intranasal route of administration compared with intraperitoneal administration. <i>Ex vivo</i> studies revealed presence of fluorescence intensity in the brain at 24 h (intranasal route), whereas in the intraperitoneal administered group signal intensity was reduced.
DOI: 10.6084/m9.figshare.27074255.v1
title: Decitabine enclosed biotin-zein conjugated nanoparticles: synthesis, characterization, <i>in vitro</i> and <i>in vivo</i> evaluation

description: <b>Aim:</b> This study focuses on biotinylated nanocarriers designed to encapsulate amphiphilic molecules with self-biodegradable properties for enhanced drug delivery. <b>Methods:</b> Biotin-zein conjugated nanoparticles were synthesized and tested in C6 cell lines to evaluate their viability and cellular uptake. Optimization was achieved using a a central composite design. The nanoparticles underwent thermogravimetric analysis, and their pharmacokinetics and biodistribution were also studied. <b>Results:</b> The optimized nanoparticles displayed 96.31% drug encapsulation efficiency, a particle size of 95.29 nm and a zeta potential of -17.7 mV. These nanoparticles showed increased cytotoxicity and improved cellular uptake compared with free drugs. Thermogravimetric analysis revealed that the drug-loaded nanocarriers provided better protection against drug degradation. Pharmacokinetic and biodistribution studies indicated that the formulation had an extended brain residence time, highlighting its effectiveness. <b>Conclusion:</b> The biotin-zein conjugated nanoparticles developed in this study offer a promising nano-vehicle for <i>in vivo</i> biodistribution and pharmacokinetic applications. Their high drug encapsulation efficiency, stability and extended brain residence time suggest they are effective for targeted drug delivery and therapeutic uses. A stable biotin and zein conjugate were formed via ethyl carbodiimide and N-hydroxy succinimide coupling to enhance site-directed delivery. Prepared polymeric conjugates were evaluated for its extent of conjugation, and secondary structural alteration revealed stability of conjugate. The biotin-zein conjugate exhibited a flexible backbone, and better encapsulation of hydrophilic molecules with a sustained release profile. Biotin-zein conjugation improved the loading capacity, and amphiphilic nature and aided the cytotoxic potential of the drug. The conjugated nanocarriers revealed the sustained release profile with higher cytotoxic potential compared with free drug along with enhanced cellular entrapment. The prepared nanoformulation revealed its safety via the nasal mucociliary canal. The formulation suggests higher residence time is observed via the intranasal route of administration compared with intraperitoneal administration. <i>Ex vivo</i> studies revealed presence of fluorescence intensity in the brain at 24 h (intranasal route), whereas in the intraperitoneal administered group signal intensity was reduced.
DOI: 10.6084/m9.figshare.27074258
title: Intratympanic injections of emulsion-like dispersions to augment cinnarizine amount in a healthy rabbit inner ear model

description: <b>Aim:</b> To investigate eutectic liquid-based emulsion-like dispersions for intratympanic injections to augment cinnarizine permeability across round window membrane in a healthy rabbit inner ear model. <b>Methods:</b> Two-tier systematic optimization was used to get the injection formula. The drug concentrations in perilymph and plasma were analyzed via. Ultra-performance liquid chromatography-tandem mass spectrometry method after 30-, 60-, 90- and 120-min post intratympanic injection time points in rabbits. <b>Results:</b> A shear-thinning behavior, immediate drug release (∼98.80%, 10 min) and higher cell viability (&gt;97.86%, 24 h) were observed in dispersions. The cinnarizine level of 8168.57 ± 1236.79 ng/ml was observed in perilymph at 30 min post intratympanic injection in rabbits. <b>Conclusion:</b> The emulsion-like dispersions can augment drug permeability through round window membrane. Intratympanic injections containing cinnarizine-loaded emulsion-like dispersions was prepared using camphor-menthol (1:0.97 molar ratio) eutectic liquid. The formula for preparing cinnarizine-loaded emulsion-like dispersions was obtained by applying linear (response surface methodology) and non-linear (artificial neural network) models. The optimized cinnarizine-loaded emulsion-like dispersions was prepared as per the formula suggested by the non-linear model. The MPS, PDI, ZP and drug amount in whole dispersions values of drug-loaded emulsion-like dispersions were 175.40 ± 5.20 nm, 0.245 ± 0.018, 43.20 ± 3.30 mV and 72.37 ± 5.67% w/v, respectively. The DEE, pH and osmolality values of drug-loaded emulsion-like dispersions prepared using the optimized formula were 96.50 ± 0.44%, 7.40 ± 0.07 and 299 ± 42.29 mOsm/kg H2O, respectively. The emulsion-like dispersions were found to be shear thinning (0.1 to 100 s-1 shear rate) and was not affected by coalescence and Ostwald ripening till 90 days at 25°C. The MTT test results performed in L-929 cells displayed cell viability values of more than 97.86% for all tested concentrations of drug-loaded emulsion-like dispersions. The presence of cinnarizine in perilymph and plasma of healthy rabbit inner ear model after intratympanic administration of drug-loaded emulsion-like dispersions was checked using a UPLC-MS/MS bioanalytical method. The intratympanic administration of drug-loaded emulsion-like dispersions displayed cinnarizine concentrations of 470.10 to 8168.57 ng/ml in perilymph for 30–120 min.
DOI: 10.6084/m9.figshare.27074258.v1
title: Intratympanic injections of emulsion-like dispersions to augment cinnarizine amount in a healthy rabbit inner ear model

description: <b>Aim:</b> To investigate eutectic liquid-based emulsion-like dispersions for intratympanic injections to augment cinnarizine permeability across round window membrane in a healthy rabbit inner ear model. <b>Methods:</b> Two-tier systematic optimization was used to get the injection formula. The drug concentrations in perilymph and plasma were analyzed via. Ultra-performance liquid chromatography-tandem mass spectrometry method after 30-, 60-, 90- and 120-min post intratympanic injection time points in rabbits. <b>Results:</b> A shear-thinning behavior, immediate drug release (∼98.80%, 10 min) and higher cell viability (&gt;97.86%, 24 h) were observed in dispersions. The cinnarizine level of 8168.57 ± 1236.79 ng/ml was observed in perilymph at 30 min post intratympanic injection in rabbits. <b>Conclusion:</b> The emulsion-like dispersions can augment drug permeability through round window membrane. Intratympanic injections containing cinnarizine-loaded emulsion-like dispersions was prepared using camphor-menthol (1:0.97 molar ratio) eutectic liquid. The formula for preparing cinnarizine-loaded emulsion-like dispersions was obtained by applying linear (response surface methodology) and non-linear (artificial neural network) models. The optimized cinnarizine-loaded emulsion-like dispersions was prepared as per the formula suggested by the non-linear model. The MPS, PDI, ZP and drug amount in whole dispersions values of drug-loaded emulsion-like dispersions were 175.40 ± 5.20 nm, 0.245 ± 0.018, 43.20 ± 3.30 mV and 72.37 ± 5.67% w/v, respectively. The DEE, pH and osmolality values of drug-loaded emulsion-like dispersions prepared using the optimized formula were 96.50 ± 0.44%, 7.40 ± 0.07 and 299 ± 42.29 mOsm/kg H2O, respectively. The emulsion-like dispersions were found to be shear thinning (0.1 to 100 s-1 shear rate) and was not affected by coalescence and Ostwald ripening till 90 days at 25°C. The MTT test results performed in L-929 cells displayed cell viability values of more than 97.86% for all tested concentrations of drug-loaded emulsion-like dispersions. The presence of cinnarizine in perilymph and plasma of healthy rabbit inner ear model after intratympanic administration of drug-loaded emulsion-like dispersions was checked using a UPLC-MS/MS bioanalytical method. The intratympanic administration of drug-loaded emulsion-like dispersions displayed cinnarizine concentrations of 470.10 to 8168.57 ng/ml in perilymph for 30–120 min.
DOI: 10.6084/m9.figshare.27074261
title: Autologous serum protein stabilized silver quantum clusters as host-specific antibacterial agents

description: <b>Aim:</b> To synthesize host-specific serum protein stabilized silver quantum clusters and assess their preclinical safety as potential antibacterial agents. <b>Materials & methods:</b> Ag-QC-NanoSera (Ag-QCNS) were synthesized using bovine, human and murine sera. Antibacterial efficacy was evaluated against <i>E. coli</i> (including antibiotic-resistant strain), <i>S. aureus</i> and <i>P. aeruginosa</i>. Biocompatibility, hemocompatibility and antibacterial mechanism were also investigated. Preclinical safety and biodistribution of autologous Ag-QCNS were assessed in BALB/c mice over 28 days. <b>Results:</b> Ag-QCNS showed high biocompatibility, hemocompatibility and high antibacterial activity at ∼12.72 μg/ml Ag equivalent. Intracellular ROS and bacterial membrane damage were confirmed as antibacterial mechanism. Ag-QCNS were established as preclinically safe. <b>Conclusion:</b> Ag-QCNS demonstrate potential as next-generation host-specific nanotheranostic antibacterial agents, enhancing the safety and efficacy while combating antibiotic resistance. Ag-QCNS were synthesized using bovine, human and murine sera, demonstrating the adaptability of the synthesis process with different biological sources. Ag-QCNS synthesized were ∼2 nm in size range and exhibited red fluorescence, allow for real-time monitoring of antibacterial activity, providing an additional diagnostic tool. Ag-QCNS displayed high <i>in vitro</i> biocompatibility in L929 normal fibroblast cells and <i>in vivo</i> hemocompatibility with murine RBCs. Ag-QCNS demonstrated concentration-dependent antibacterial efficacy against Gram-positive and Gram-negative bacteria, including <i>E. coli</i>, <i>S. aureus</i> and <i>P. aeruginosa</i>. The synthesized Ag-QCNS were effective against both antibiotic-sensitive and antibiotic-resistant strains of <i>E. coli</i>. The antibacterial mechanism was identified as ROS-mediated oxidative stress, causing bacterial cell membrane damage, as verified by electron microscopy. Acute and subacute preclinical safety of autologously derived murine Ag-QCNS was established over course of 28 days. Ag-QCNS has immense potential as next-generation host-specific nanotheranostic antibacterial agents by increasing the safety of antibacterial therapy and combating antibiotic-resistance.
DOI: 10.6084/m9.figshare.27074261.v1
title: Autologous serum protein stabilized silver quantum clusters as host-specific antibacterial agents

description: <b>Aim:</b> To synthesize host-specific serum protein stabilized silver quantum clusters and assess their preclinical safety as potential antibacterial agents. <b>Materials & methods:</b> Ag-QC-NanoSera (Ag-QCNS) were synthesized using bovine, human and murine sera. Antibacterial efficacy was evaluated against <i>E. coli</i> (including antibiotic-resistant strain), <i>S. aureus</i> and <i>P. aeruginosa</i>. Biocompatibility, hemocompatibility and antibacterial mechanism were also investigated. Preclinical safety and biodistribution of autologous Ag-QCNS were assessed in BALB/c mice over 28 days. <b>Results:</b> Ag-QCNS showed high biocompatibility, hemocompatibility and high antibacterial activity at ∼12.72 μg/ml Ag equivalent. Intracellular ROS and bacterial membrane damage were confirmed as antibacterial mechanism. Ag-QCNS were established as preclinically safe. <b>Conclusion:</b> Ag-QCNS demonstrate potential as next-generation host-specific nanotheranostic antibacterial agents, enhancing the safety and efficacy while combating antibiotic resistance. Ag-QCNS were synthesized using bovine, human and murine sera, demonstrating the adaptability of the synthesis process with different biological sources. Ag-QCNS synthesized were ∼2 nm in size range and exhibited red fluorescence, allow for real-time monitoring of antibacterial activity, providing an additional diagnostic tool. Ag-QCNS displayed high <i>in vitro</i> biocompatibility in L929 normal fibroblast cells and <i>in vivo</i> hemocompatibility with murine RBCs. Ag-QCNS demonstrated concentration-dependent antibacterial efficacy against Gram-positive and Gram-negative bacteria, including <i>E. coli</i>, <i>S. aureus</i> and <i>P. aeruginosa</i>. The synthesized Ag-QCNS were effective against both antibiotic-sensitive and antibiotic-resistant strains of <i>E. coli</i>. The antibacterial mechanism was identified as ROS-mediated oxidative stress, causing bacterial cell membrane damage, as verified by electron microscopy. Acute and subacute preclinical safety of autologously derived murine Ag-QCNS was established over course of 28 days. Ag-QCNS has immense potential as next-generation host-specific nanotheranostic antibacterial agents by increasing the safety of antibacterial therapy and combating antibiotic-resistance.
DOI: 10.15468/dl.6kxt2r
title: Occurrence Download

description: A dataset containing 3157 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-8.71396 42.38178,-8.24819 42.38178,-8.24819 42.38936,-8.24819 42.6593,-8.68096 42.66417,-8.71396 42.38178))",
 "TaxonKey is Mammalia"
 ]
}

 The dataset includes 3157 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024333-240906103802322/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.11107653
title: CSRM-derived: A 3-D crustal composition model in continental China

description: This dataset contains a three-dimensional crustal composition model of the SiO2 content in the crustal bedrocks beneath continental China, derived from the CSRM-1.0 (model link). The spatial resolution of the composition model is ~60 km in the north-south seismic belt and trans-North China Orogen, and ~120 km in the rest of the continental China. This composition model results from a project of constructing the high-resolution China Seismological Reference Model (CSRM-1.0) in the top 100 km of the crust and uppermost mantle in continental China (Xiao et al, JGR, 2024).

本数据库包含了利用 CSRM-1.0(模型链接)估算的中国大陆区域地壳内不同深度岩石内二氧化硅含量的模型。该模型在南北地震带和华北造山带的分辨率约为 60 km,在中国大陆的其他区域约为 120 km。该成分模型源于构建中国大陆区域高精度地壳和上地幔顶部 100 公里三维地震学模型 (CSRM-1.0) 的工作 (Xiao et al, JGR, 2024) 。
DOI: 10.5281/zenodo.11107654
title: CSRM-derived: A 3-D crustal composition model in continental China

description: This dataset contains a three-dimensional crustal composition model of the SiO2 content in the crustal bedrocks beneath continental China, derived from the CSRM-1.0 (model link). The spatial resolution of the composition model is ~60 km in the north-south seismic belt and trans-North China Orogen, and ~120 km in the rest of the continental China. This composition model results from a project of constructing the high-resolution China Seismological Reference Model (CSRM-1.0) in the top 100 km of the crust and uppermost mantle in continental China (Xiao et al, JGR, 2024).

本数据库包含了利用 CSRM-1.0(模型链接)估算的中国大陆区域地壳内不同深度岩石内二氧化硅含量的模型。该模型在南北地震带和华北造山带的分辨率约为 60 km,在中国大陆的其他区域约为 120 km。该成分模型源于构建中国大陆区域高精度地壳和上地幔顶部 100 公里三维地震学模型 (CSRM-1.0) 的工作 (Xiao et al, JGR, 2024) 。
DOI: 10.5281/zenodo.10530032
title: Strategies to mitigate differences between environments using Rapid Review on Grey Literature

description: The comparison spreadsheet serves as a comprehensive repository for organizing and synthesizing data extracted from Grey Literature sources. It contains coded information related to strategies for mitigating configuration differences in software development, allowing for a systematic analysis of the identified practices. The spreadsheet links to different sections of the paper by providing a structured framework for data analysis. In the 'Data synthesis' section, the spreadsheet supports a mixed-method approach, combining qualitative and quantitative methods for analyzing the data. It aids in thematic analysis by translating codes into themes and creating higher-order themes for a more in-depth understanding of the collected data. Additionally, the spreadsheet connects to the 'Extract and Code data' section, where it houses all relevant codes and facilitates discussions on pertinent information extracted from Grey Literature sources. By referencing unique identifiers within the spreadsheet, researchers can track and link specific entries to the research questions, ensuring alignment with the study's objectives. Overall, the comparison spreadsheet plays a pivotal role in organizing, analyzing, and synthesizing data from Grey Literature sources, contributing to the robustness and coherence of the research findings presented in the paper.
DOI: 10.5281/zenodo.13816541
title: (DATASET+REPLICATION) Does more knowledge on anti-discrimination law relate to more support for anti-discrimination policy on the rental housing market?
title: Data + Rscript for replication

description: In line with the conditions outlined in the data management plan of the EdisTools project, of which this study is a part, the anonymised data will be made available after five years following the end of the research project. This approach guarantees the research team’s possibilities to fully exploit the data for scientific valorisation and to obtain academic qualifications. Minimal, anonymised data will be unconditionally made available upon request but solely to replicate the study’s findings.
DOI: 10.5281/zenodo.13816542
title: (DATASET+REPLICATION) Does more knowledge on anti-discrimination law relate to more support for anti-discrimination policy on the rental housing market?
title: Data + Rscript for replication

description: In line with the conditions outlined in the data management plan of the EdisTools project, of which this study is a part, the anonymised data will be made available after five years following the end of the research project. This approach guarantees the research team’s possibilities to fully exploit the data for scientific valorisation and to obtain academic qualifications. Minimal, anonymised data will be unconditionally made available upon request but solely to replicate the study’s findings.
DOI: 10.5281/zenodo.10425668
title: Strategies to mitigate differences between environments using Rapid Review on Grey Literature

description: Dataset for the study that aims to provide insights and best practices for mitigating configuration differences in software development through a rapid review of grey literature.