cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6318668
title: On the Machinability and Surface Finish of Superalloy GH909 Under Dry Cutting Conditions

description: GH 909 alloy is a kind of low thermal expansion superalloy with high strength and low expansion coefficient. It is mainly used for manufacturing of aero-engine turbine casings, sealing ring, vanes and other gap control parts, to improve the working efficiency and thrust of the engine as well as to reduce gas loss and fuel consumption. However, the machinability is still a problem that restricts the application of GH909. This paper investigated the machinability referring to cutting forces, surface quality and tool wear in the milling process with carbide tool. The main conclusions are: (1) GH909 has similar machinability with other Nickel-based alloys, cutting velocity does not have great influence on cutting forces for small radial cutting depth. (2) Good machined surface quality can be get for cutting velocity around 70m/min. (3) To get good tool performance and reduce tool wear rate, cutting velocity can be set to no larger than 80m/min.
DOI: 10.6084/m9.figshare.6318668.v1
title: On the Machinability and Surface Finish of Superalloy GH909 Under Dry Cutting Conditions

description: GH 909 alloy is a kind of low thermal expansion superalloy with high strength and low expansion coefficient. It is mainly used for manufacturing of aero-engine turbine casings, sealing ring, vanes and other gap control parts, to improve the working efficiency and thrust of the engine as well as to reduce gas loss and fuel consumption. However, the machinability is still a problem that restricts the application of GH909. This paper investigated the machinability referring to cutting forces, surface quality and tool wear in the milling process with carbide tool. The main conclusions are: (1) GH909 has similar machinability with other Nickel-based alloys, cutting velocity does not have great influence on cutting forces for small radial cutting depth. (2) Good machined surface quality can be get for cutting velocity around 70m/min. (3) To get good tool performance and reduce tool wear rate, cutting velocity can be set to no larger than 80m/min.
DOI: 10.6084/m9.figshare.6318803
title: microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb

description: Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A significant up-regulation of miR-3129 was observed in GC tissues compared to adjacent tissues. Overexpression of miR-3129 significantly improved cell viability after 4 days of post-transfection. Flow cytometry assay results showed that the miR-3129 overexpression arrested more SGC7901 cells at S phase. Moreover, overexpression of miR-3129 down-regulated the expression of CDK2 inhibitors while it up-regulated the expression levels of cyclin E, CDK2, and pRb. Interestingly, we found that pRb inhibition reversed the effect of miR-3129 inhibitor on cell proliferation in SGC7901 cells, increased cell viability, reduced cells at G0/1 phase, and modulated the expression of proliferation-related factors. Our results revealed that miR-3129 functioned as an oncogene through positive regulation of pRb and may prove to be a promising option for molecular therapy of GC.
DOI: 10.6084/m9.figshare.6318803.v1
title: microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb

description: Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A significant up-regulation of miR-3129 was observed in GC tissues compared to adjacent tissues. Overexpression of miR-3129 significantly improved cell viability after 4 days of post-transfection. Flow cytometry assay results showed that the miR-3129 overexpression arrested more SGC7901 cells at S phase. Moreover, overexpression of miR-3129 down-regulated the expression of CDK2 inhibitors while it up-regulated the expression levels of cyclin E, CDK2, and pRb. Interestingly, we found that pRb inhibition reversed the effect of miR-3129 inhibitor on cell proliferation in SGC7901 cells, increased cell viability, reduced cells at G0/1 phase, and modulated the expression of proliferation-related factors. Our results revealed that miR-3129 functioned as an oncogene through positive regulation of pRb and may prove to be a promising option for molecular therapy of GC.
DOI: 10.6084/m9.figshare.6318806.v1
title: lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line

description: Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. FTC-133 cells were transfected with CCAT1 expressing vector, CCAT1 shRNA, miR-143 mimic, and miR-143 inhibitor, respectively. After different treatments, cell viability, proliferation, migration, invasion, and apoptosis were measured. Moreover, the regulatory relationship of CCAT1 and miR-143, as well as miR-143 and VEGF were tested using dual-luciferase reporter assay. The relative expressions of CCAT1, miR-143, and VEGF were tested by qRT-PCR. The expressions of apoptosis-related factors and corresponding proteins in PI3K/AKT and MAPK pathways were analyzed using western blot analysis. The results suggested that CCAT1 was up-regulated in the FTC-133 cells. CCAT1 suppression decreased FTC-133 cell viability, proliferation, migration, invasion, and miR-143 expression, while it increased apoptosis and VEGF expression. CCAT1 might act as a competing endogenous RNA (ceRNA) for miR-143. Moreover, CCAT1 activated PI3K/AKT and MAPK signaling pathways through inhibition of miR-143. This study demonstrated that CCAT1 exhibited pro-proliferative and pro-metastasis functions on FTC-133 cells and activated PI3K/AKT and MAPK signaling pathways via down-regulation of miR-143. These findings will provide a possible target for clinical treatment of thyroid cancer.
DOI: 10.6084/m9.figshare.6318806
title: lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line

description: Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. FTC-133 cells were transfected with CCAT1 expressing vector, CCAT1 shRNA, miR-143 mimic, and miR-143 inhibitor, respectively. After different treatments, cell viability, proliferation, migration, invasion, and apoptosis were measured. Moreover, the regulatory relationship of CCAT1 and miR-143, as well as miR-143 and VEGF were tested using dual-luciferase reporter assay. The relative expressions of CCAT1, miR-143, and VEGF were tested by qRT-PCR. The expressions of apoptosis-related factors and corresponding proteins in PI3K/AKT and MAPK pathways were analyzed using western blot analysis. The results suggested that CCAT1 was up-regulated in the FTC-133 cells. CCAT1 suppression decreased FTC-133 cell viability, proliferation, migration, invasion, and miR-143 expression, while it increased apoptosis and VEGF expression. CCAT1 might act as a competing endogenous RNA (ceRNA) for miR-143. Moreover, CCAT1 activated PI3K/AKT and MAPK signaling pathways through inhibition of miR-143. This study demonstrated that CCAT1 exhibited pro-proliferative and pro-metastasis functions on FTC-133 cells and activated PI3K/AKT and MAPK signaling pathways via down-regulation of miR-143. These findings will provide a possible target for clinical treatment of thyroid cancer.
DOI: 10.6084/m9.figshare.6319559
title: Additional file 1: of GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

description: Raw data of 2143 simples. Clinical specimens test results from the infectious diseases hospital of Changchun city from January to December 2016. (XLSX 65Â kb)
DOI: 10.6084/m9.figshare.6319559.v1
title: Additional file 1: of GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

description: Raw data of 2143 simples. Clinical specimens test results from the infectious diseases hospital of Changchun city from January to December 2016. (XLSX 65Â kb)
DOI: 10.6084/m9.figshare.25469461
title: crosslinks_Ago2IP_miR181a/b-1-KO_plus.bw

description: Non-chimeric crosslinks of Ago2 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Knockout of miR181a/b-1, merge of all 3 replicates, crosslinks from plus strand.
DOI: 10.6084/m9.figshare.25469461.v1
title: crosslinks_Ago2IP_miR181a/b-1-KO_plus.bw

description: Non-chimeric crosslinks of Ago2 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Knockout of miR181a/b-1, merge of all 3 replicates, crosslinks from plus strand.
DOI: 10.6084/m9.figshare.25469464
title: crosslinks_miR181enr_plus.bw

description: Non-chimeric crosslinks of Ago2 with enrichment for miR181 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from plus strand.
DOI: 10.6084/m9.figshare.25469464.v1
title: crosslinks_miR181enr_plus.bw

description: Non-chimeric crosslinks of Ago2 with enrichment for miR181 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from plus strand.
DOI: 10.6084/m9.figshare.25469467
title: crosslinks_miR181enr_minus.bw

description: Non-chimeric crosslinks of Ago2 with enrichment for miR181 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from minus strand.
DOI: 10.6084/m9.figshare.25469467.v1
title: crosslinks_miR181enr_minus.bw

description: Non-chimeric crosslinks of Ago2 with enrichment for miR181 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from minus strand.
DOI: 10.1285/i20380313v36p73
title: Anseau de Toucy comes Soleti e la comunità ellenofona del Salento nella prima Età angioina

description: The article deals with the biography of Anseau de Toucy, a knight of French origin but born in Constantinople, of Greek mother, during the sixty years of the Latin Empire. The biographical traces, recoverable through historiographical sources, that link him to the Polis, to the Principality of Achaia during the rule of his brother-in-law Guillame de Villehardouin, and finally to the Angevin kingdom of Sicily are highlighted. His Franco-Greek ethnicity, common to other Angevin feudal lords who received lands in the Greek-speaking South Salento, is emphasized. The Angevin Age could, in fact, represent for Southern Apulia an important moment of contact with the Greek East. This would have allowed the spoken Greek language (the Grìco) in the Salento (still present today) to continue to exist, thanks to new arrivals of hellenophone peoples following the Frankish-Greek feudal lords. Some linguistic elements that seem to support this thesis are brought to attention.
description: L'articolo affronta la biografia di Anseau de Toucy, cavaliere di origine francese ma nato a Costantinopoli, da madre greca, durante il sessantennio dell'Impero latino. Vengono messe in evidenza le tracce biografiche, recuperabili attraverso le fonti storiografiche, che lo legano alla Polis, al Principato di Acaia durante la signoria di suo cognato Guillame de Villehardouin, e, infine, al regno angioino di Sicilia. Si mette in risalto la sua etnia franco-greca, comune ad altri feudatari angioini che ricevettero terre nel Sud Salento ellenofono. L'Età angioina potrebbe, infatti, rappresentare un importante momento di contatto con l'Oriente greco. Ciò avrebbe consentito al greco parlato (il grìco) nel Salento (ancora oggi) di continuare ad esistere, grazie a nuovi arrivi di genti ellenofone al seguito dei feudatari franco-greci. Vengono posti all'attenzione alcuni elementi linguistici che sembrano sostenere questa tesi.
DOI: 10.6084/m9.figshare.25469470.v1
title: crosslinks_Ago2IP_plus.bw

description: Non-chimeric crosslinks of Ago2 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from plus strand.
DOI: 10.6084/m9.figshare.25469470
title: crosslinks_Ago2IP_plus.bw

description: Non-chimeric crosslinks of Ago2 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from plus strand.
DOI: 10.34740/kaggle/dsv/7936252
title: 💻 🇵🇭 PCHub online PC parts prices
DOI: 10.5281/zenodo.10867512
title: Data required for "Low mutation rate of spontaneous mutants enables detection of causative genes by comparing whole genome sequences"

description: In the early 1900s,mutation breeding to select varieties with desirable traits using  spontaneous mutation was actively conducted around the world, including Japan. In rice, the number of fixed mutations per generation was estimated to be 1.38-2.25. Although this low mutation rate was a major problem for breeding in those days, in the modern era with the development of NGS technology, it was conversely considered to be an advantage for efficient gene identification. In this paper, we proposed an in silico approach using next-generation sequencing (NGS) to compare the whole genome sequence of a spontaneous mutant with that of a closely related strain with a nearly identical genome, to find polymorphisms that differ between them, and to identify the causal gene by predicting the functional variation of the gene caused by the polymorphism. Using this approach, we found four causal genes for the dwarf mutation, the round shape grain mutation and the awnless mutation. Three of these genes were the same as those previously reported, but one was a novel gene involved in awn formation. The novel gene was isolated from Bozu-Aikoku, a mutant of Aikoku with the awnless trait, in which nine polymorphisms were predicted to alter gene function by their whole-genome comparison. Based on the information on gene function and tissue-specific expression patterns of these candidate genes, Os03g0115700/LOC_Os03g02460, annotated as a shortchain dehydrogenase/reductase SDR family protein, is most likely to be involved in the awnless mutation. Indeed, complementation tests by transformation showed that it is involved in awn formation. Thus, this method is an effective way to accelerate genome breeding of various crop species by enabling the identification of useful genes that can be used for crop breeding with minimal effort for NGS analysis.
DOI: 10.5281/zenodo.10867513
title: Data required for "Low mutation rate of spontaneous mutants enables detection of causative genes by comparing whole genome sequences"

description: In the early 1900s,mutation breeding to select varieties with desirable traits using  spontaneous mutation was actively conducted around the world, including Japan. In rice, the number of fixed mutations per generation was estimated to be 1.38-2.25. Although this low mutation rate was a major problem for breeding in those days, in the modern era with the development of NGS technology, it was conversely considered to be an advantage for efficient gene identification. In this paper, we proposed an in silico approach using next-generation sequencing (NGS) to compare the whole genome sequence of a spontaneous mutant with that of a closely related strain with a nearly identical genome, to find polymorphisms that differ between them, and to identify the causal gene by predicting the functional variation of the gene caused by the polymorphism. Using this approach, we found four causal genes for the dwarf mutation, the round shape grain mutation and the awnless mutation. Three of these genes were the same as those previously reported, but one was a novel gene involved in awn formation. The novel gene was isolated from Bozu-Aikoku, a mutant of Aikoku with the awnless trait, in which nine polymorphisms were predicted to alter gene function by their whole-genome comparison. Based on the information on gene function and tissue-specific expression patterns of these candidate genes, Os03g0115700/LOC_Os03g02460, annotated as a shortchain dehydrogenase/reductase SDR family protein, is most likely to be involved in the awnless mutation. Indeed, complementation tests by transformation showed that it is involved in awn formation. Thus, this method is an effective way to accelerate genome breeding of various crop species by enabling the identification of useful genes that can be used for crop breeding with minimal effort for NGS analysis.
DOI: 10.6084/m9.figshare.25469473
title: crosslinks_Ago2IP_minus.bw

description: Non-chimeric crosslinks of Ago2 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from minus strand.
DOI: 10.6084/m9.figshare.25469473.v1
title: crosslinks_Ago2IP_minus.bw

description: Non-chimeric crosslinks of Ago2 in mouse thymocytes, from miR181-eCLIP experiment in Verheyden & Klostermann et al 2024. Merge of all 3 replicates, crosslinks from minus strand.
DOI: 10.5281/zenodo.10867775
title: Elucidating the Polymorphism of Xanthone: A Crystallization and Characterization Study

description: Dataset related to the publication: "Elucidating the Polymorphism of Xanthone: A Crystallization and Characterization Study". Accepted in Crystal Growth and Design on February 9th, 2024.

Abstract: The aim of this work is to shed light on the polymorphism of xanthones, a class of oxygenated molecules well known for their bioactivity, including antioxidant, anticancer, and anti-inflammatory effects. Understanding the polymorphism of xanthones can enable the design of novel solid products for pharmaceutical, nutraceutical, and agrochemical applications. Prior to this work, two entries accounting for different space groups were deposited for 9-xanthone in the Cambridge Structure Database (CSD): an orthorhombic P212121 and a monoclinic P21 structure solved at room and low temperatures, respectively. However, the very high similarity between these two structures and the lack of clear differences in the physical properties (e.g., thermal behavior) suggested the possibility of the existence of only one crystal structure. In fact, the differences shown in the literature data might be related to the chosen operating parameters, as well as the instrumental resolution of the single-crystal X-ray diffraction experiments. In the work presented here, the ambiguity in the polymorphism of xanthone is investigated using thermal analysis, powder and synchrotron single-crystal XRD, and optical microscopy. Additionally, a workflow for the correct identification of twinned crystal structures, which can be applied to other polymorphic systems, is presented. Such workflow combines the collection of a large data set of high-resolution diffraction patterns using synchrotron radiation with the use of principal component analysis, a dimensionality reduction technique, for a quick and effective identification of phase transitions happening during the data collection. Crystallization experiments were designed to promote the formation of different crystal structures of xanthone that were recrystallized based on past literature and beyond.
DOI: 10.5281/zenodo.10867776
title: Elucidating the Polymorphism of Xanthone: A Crystallization and Characterization Study

description: Dataset related to the publication: "Elucidating the Polymorphism of Xanthone: A Crystallization and Characterization Study". Accepted in Crystal Growth and Design on February 9th, 2024.

Abstract: The aim of this work is to shed light on the polymorphism of xanthones, a class of oxygenated molecules well known for their bioactivity, including antioxidant, anticancer, and anti-inflammatory effects. Understanding the polymorphism of xanthones can enable the design of novel solid products for pharmaceutical, nutraceutical, and agrochemical applications. Prior to this work, two entries accounting for different space groups were deposited for 9-xanthone in the Cambridge Structure Database (CSD): an orthorhombic P212121 and a monoclinic P21 structure solved at room and low temperatures, respectively. However, the very high similarity between these two structures and the lack of clear differences in the physical properties (e.g., thermal behavior) suggested the possibility of the existence of only one crystal structure. In fact, the differences shown in the literature data might be related to the chosen operating parameters, as well as the instrumental resolution of the single-crystal X-ray diffraction experiments. In the work presented here, the ambiguity in the polymorphism of xanthone is investigated using thermal analysis, powder and synchrotron single-crystal XRD, and optical microscopy. Additionally, a workflow for the correct identification of twinned crystal structures, which can be applied to other polymorphic systems, is presented. Such workflow combines the collection of a large data set of high-resolution diffraction patterns using synchrotron radiation with the use of principal component analysis, a dimensionality reduction technique, for a quick and effective identification of phase transitions happening during the data collection. Crystallization experiments were designed to promote the formation of different crystal structures of xanthone that were recrystallized based on past literature and beyond.