DOI: 10.22002/rkxnc-zke34
title: Caulobacter crescentus

description: wild type CB15N. not included in paper? . extended stationary phase, ATCC PYE formula, stressed cells, 2nd bacterium found
description: <strong>Tilt Series Date:</strong> 2004-06-04</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Dual Axis, tilt range: (-61.0°, 64.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep & data collection: D. Prabha Dias; processing: Bingni Brunton (prev. Wen)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> extended stationary phase, tapwater ATCC (PYE) media, wild-type CB15N</p>
<strong>Sample Preparation:</strong> Quantifoil R2/1, 10nm gold on grids & in suspension, 100% humidity, -3 offset, 1 sec blot, 2 sec drain, gold on </p>
  pdd2004-06-04-2_slicer4657.jpg: Granule pdd2004-06-04-2_slicer4658.jpg: Peptidoglycan pdd2004-06-04-2_slicer4659.jpg: Stacked membrane pdd2004-06-04-2_slicer4660.jpg: Granule pdd2004-06-04-2_slicer4662.jpg: S-layer pdd2004-06-04-2_slicer4667.jpg: Flat vesicle pdd2004-06-04-2_slicer4669.jpg: Unknown ring pdd2004-06-04-2_slicer4671.jpg: Peptidoglycan one layer
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-2/rawdata/</p>CC04Jun04e100t02a.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-2/rawdata/CC04Jun04e100t02a.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC04Jun04e100t02b.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-2/rawdata/CC04Jun04e100t02b.mrc"
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        bytenorm2a.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-2/3dimage_189/bytenorm2a.rec"
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        bytenorm2b.rec,  Reconstruction (Pixel Size 2.4 nm), 608.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-2/3dimage_190/bytenorm2b.rec"
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        sum.rec,  Reconstruction (Pixel Size 2.4 nm) Analysis files associated to 3D Image #191 have not been uploaded into the Database. .  Original directory: pd2004-06-04-02/Reconstructions/Full/Analysis/ .  Notes for Full: Keywords: .  stationary phase .  100 electrons dose .  CB15 .  GIFCCD, 734.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-04-2/3dimage_191/sum.rec"
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        brightCCNAD_EE25.mov,  file associated to 3D Image #191, 6.9 MB  
        
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        CC04Jun_a.mov,  file associated to 3D Image #191, 23.7 MB  
        
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        CC04Jun_b.mov,  file associated to 3D Image #191, 22.8 MB  
        
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        CCfullrec.mov,  file associated to 3D Image #191, 86.4 MB  
        
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        CCNAD_EE25.mov,  file associated to 3D Image #191, 11.3 MB  
        
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