cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20039097
title: Design and preparation of a novel colon-targeted tablet of hydrocortisone

description: ABSTRACT The objective of this research was to design a new colon-targeted drug delivery system based on chitosan. The properties of the films were studied to obtain useful information about the possible applications of composite films. The composite films were used in a bilayer system to investigate their feasibility as coating materials. Tensile strength, swelling degree, solubility, biodegradation degree, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Scanning Electron Microscope (SEM) investigations showed that the composite film was formed when chitosan and gelatin were reacted jointly. The results showed that a 6:4 blend ratio was the optimal chitosan/gelatin blend ratio. In vitro drug release results indicated that the Eudragit- and chitosan/gelatin-bilayer coating system prevented drug release in simulated intestinal fluid (SIF) and simulated gastric fluid (SGF). However, the drug release from a bilayer-coated tablet in SCF increased over time, and the drug was almost completely released after 24h. Overall, colon-targeted drug delivery was achieved by using a chitosan/gelatin complex film and a multilayer coating system.
DOI: 10.6084/m9.figshare.7980656
title: Additional file 13: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S7. Different expressed transcription factors in transcriptome analysis. (XLS 6892 kb)
DOI: 10.6084/m9.figshare.20039337.v1
title: Antioxidant and immunomodulatory activities of Oviductus ranae in mice

description: Oviductus ranae (OR) is a traditional Chinese medicine, which was first recorded in the Compendium of Materia Medica in the Ming Dynasty. OR contains high amounts of proteins and elicits therapeutic effects on neurasthenia, insomnia, and respiratory symptoms, which are related to oxidative stress and immunodeficiency. This study aimed to obtain the potential of OR for the development of functional food possessing antioxidant and immune-enhancement functions in the same dose. In antioxidant evaluation, OR can significantly decrease malondialdehyde and protein carbonyls (P &lt; 0.05, P &lt; 0.01) and significantly increase total superoxide dismutase and glutathione in a dose-dependent manner (P&lt; 0.05, P &lt; 0.01) against ethanol-induced oxidative stress in mice at 0.1, 1.0, and 3.0 g/kg BW. In immunomodulatory evaluation, OR could significantly enhance the phagocytosis of liver macrophages (P &lt; 0.05, P &lt; 0.01), delayed-type hypersensitivity response (P &lt; 0.05, P &lt; 0.01), hemolytic activity (P &lt; 0.05), antibody-producing cells (P &lt; 0.05), and natural killer cell activity (P &lt; 0.05) in the same dose range described in antioxidant evaluation compared with those in the normal control. OR slightly influenced lymphocyte proliferation, peritoneal macrophage phagocytosis, and immune organ indices in mice. Thus, 3.0 g/kg BW OR showed potential for the development of functional food with antioxidant and immune-enhancement activities.
DOI: 10.6084/m9.figshare.16893390
title: Additional file 9 of Genome-wide association study reveals 14 new SNPs and confirms two structural variants highly associated with the horned/polled phenotype in goats

description: Additional file 9: Table S6: The information of PCR primer pairs used in this study.
DOI: 10.6084/m9.figshare.20039304.v1
title: Pharmacokinetics and safety of repirinast tablets in healthy Chinese subjects

description: ABSTRACT Repirinast is a new, synthetic, disodium cromoglycate-like antiallergic agent for oral administration in humans. This study evaluated the safety, tolerability and pharmacokinetics of repirinast tablets in healthy Chinese volunteers. This was a phase I, open-label, randomized, single- and multiple-dose study. Subjects were assigned to receive a single dose of repirinast tablet at either 150, 300, or 450 mg, or multiple doses of 150 mg twice daily for 5 days. Plasma samples were analyzed with LC-MS/MS. Pharmacokinetic parameters of active metabolite MY-1250 (deesterified repirinast) were calculated using non-compartmental analysis with WinNonlin software. Statistical analysis was performed using SPSS software. All adverse events (AEs) were mild and of limited duration. No serious adverse event (SAE), death or withdrawal from the study was observed. In the single-dose study, Cmax was reached at about 0.75 hour, and the mean t1/2 was approximately 16.21 hours. Area under curve (AUC) and Cmax increased with dose escalation, but dose proportionality was not observed over the range of 150 to 450 mg. In the multiple-dose study, the steady-state was reached within 3 days with no accumulation. Repirinast tablet was well tolerated in healthy Chinese subjects.
DOI: 10.6084/m9.figshare.16893366.v1
title: Additional file 1 of Genome-wide association study reveals 14 new SNPs and confirms two structural variants highly associated with the horned/polled phenotype in goats

description: Additional file 1: Table S1: Summary of mapping statistics for 45 JT goats in this study.
DOI: 10.6084/m9.figshare.20039106.v1
title: Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination

description: Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.
DOI: 10.6084/m9.figshare.20039400.v1
title: Preparation and evaluation of 2-methoxyestradiol-loaded pH-sensitive liposomes

description: The development and clinical application of 2-methoxyestradiol (2-ME) as a new type of antitumor drug are limited due to its poor solubility, rapid metabolism in vivo, and large oral dosage. 2-ME-loaded pH-sensitive liposomes (2-ME-PSLs) was prepared containing the lipids, Lipoid E-80 (E-80), cholesteryl hemisuccinate (CHEMS), and cholesterol (CHOL) via thin-film ultrasonic dispersion. First, preparation conditions of 2-ME-PSLs were optimized by orthogonal test. Then 2-ME-PSL was characterized, and the release behavior and stability of 2-ME-PSL in vitro were evaluated. The optimal preparation conditions for 2-ME-PSLs were as follows: 2-ME : E-80+CHEMS 1:15; CHOL : E-80+CHEMS 1:5; ultrasonication time 20 minutes. The mean particle size, PDI, zeta potential, and entrapment efficiency (EE) of 2-ME-PSLs were 116 ± 9 nm, 0.161 ± 0.025, −22.4 ± 1.7 mV, and 98.6 ± 0.5%, respectively. As viewed under a transmission electron microscope, 2-ME-PSLs were well dispersed and almost spherical. They exhibited significant pH-sensitive properties and were fairly stable when diluted with a physiological solution. In conclusion, 2-ME-PSLs were successfully prepared and possessed a favorable pH sensitivity and good dissolution stability with a normal solution.
DOI: 10.6084/m9.figshare.7980794
title: MOESM1 of Small nucleolar RNA Sf-15 regulates proliferation and apoptosis of Spodoptera frugiperda Sf9 cells

description: Additional file 1: Table S1. Differentially expressed gene list upon Sf-15 overexpression and repression. ASO and Sf-15 means cells were transfected with antisense oligonucleotides of Sf-15 and overexpression vector pBac[A3-EGFP-A3-Sf-15], respectively.
DOI: 10.6084/m9.figshare.20039274.v1
title: Marsdenia tenacissima extract alters crucial metabolites in cancer, determined by 1 H NMR based metabolomics approach

description: ABSTRACT Altered metabolites level in the biosystems, is the potential cause of cancer, the primary reason of alteration of metabolism is change in nutrient consumption and waste excretion, as a result genetic mutation leads to cancer initiation and progression. Aberration of specific metabolites such as fumarate, succinate, 2-hydroxyglutarate may alter cell signaling. We collected liver and kidney samples and prepared for 1H NMR analysis, then executed NMR spectroscopy. We used a set of domestic R scripts to perform an unsupervised principal component analysis (PCA) and a supervised orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA). It signifies class discrimination for getting a clear separation, whereas PCA scores plot signifies the model group kept further away from the control group than drug group on the horizontal axis. In another PCA scores plots, most parts of the control group was overlapping with the drug group but was distant from the model group. Marsdenia tenacissima extract (MTE) (Chines name: Xiao-Ai-Ping, XAP) modulates level of crucial metabolites such as fumarate, lactate, succinate, determined by 1H NMR spectroscopy and their altered level contributes major role in cancer.
DOI: 10.6084/m9.figshare.20039106
title: Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination

description: Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.
DOI: 10.6084/m9.figshare.20039431
title: Effect of prodigiosin on the alleviation of the intestinal inflammation of weaned rats based on 1H-NMR spectroscopy study and biochemistry indexes

description: Weaning results in intestinal dysfunction, mucosal atrophy, transient anorexia, and intestinal barrier defects. In this study, the effect of prodigiosin (PG) on the intestinal inflammation of weaned rats was investigated by using 1H-NMR spectroscopy and biochemistry indexes to regulate the intestinal metabolism. After administration for 14 days, the body mass of the PG group was increased by 1.29- and 1.26-fold compared with those of the control and alcohol groups, respectively, using a dose of 200 µg PG·kg-1 body weight per day. PG increased organic acid content and decreased moisture, pH values, and free ammonia in feces. In addition, PG alleviated the intestinal inflammation of weaned rats. The analysis of 1H-NMR signal peak attribution and the model validation of metabolic data of feces contents showed that PG significantly affected the metabolism of small molecular compounds in the intestinal tract of weaned rats. This study presents the promising alternative of using PG to alleviate intestinal inflammation effectively in the intestinal tract of weaned rats.
DOI: 10.6084/m9.figshare.7980665
title: Additional file 15: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S8. Primer pairs used in real-time quantitative RT-PCR. (XLS 35 kb)
DOI: 10.6084/m9.figshare.7980674
title: Additional file 2: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S1. Gene description and expression in transcriptome. (XLSX 75423 kb)
DOI: 10.6084/m9.figshare.7980674.v1
title: Additional file 2: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S1. Gene description and expression in transcriptome. (XLSX 75423 kb)
DOI: 10.6084/m9.figshare.7980707
title: Additional file 5: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S3. The detailed information of unigenes in each GO functional category of transcriptome analysis. (XLS 72 kb)
DOI: 10.6084/m9.figshare.7980719.v1
title: Additional file 7: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S4. Correlation between transcriptome and proteome. (XLSX 1344 kb)
DOI: 10.6084/m9.figshare.20039337
title: Antioxidant and immunomodulatory activities of Oviductus ranae in mice

description: Oviductus ranae (OR) is a traditional Chinese medicine, which was first recorded in the Compendium of Materia Medica in the Ming Dynasty. OR contains high amounts of proteins and elicits therapeutic effects on neurasthenia, insomnia, and respiratory symptoms, which are related to oxidative stress and immunodeficiency. This study aimed to obtain the potential of OR for the development of functional food possessing antioxidant and immune-enhancement functions in the same dose. In antioxidant evaluation, OR can significantly decrease malondialdehyde and protein carbonyls (P &lt; 0.05, P &lt; 0.01) and significantly increase total superoxide dismutase and glutathione in a dose-dependent manner (P&lt; 0.05, P &lt; 0.01) against ethanol-induced oxidative stress in mice at 0.1, 1.0, and 3.0 g/kg BW. In immunomodulatory evaluation, OR could significantly enhance the phagocytosis of liver macrophages (P &lt; 0.05, P &lt; 0.01), delayed-type hypersensitivity response (P &lt; 0.05, P &lt; 0.01), hemolytic activity (P &lt; 0.05), antibody-producing cells (P &lt; 0.05), and natural killer cell activity (P &lt; 0.05) in the same dose range described in antioxidant evaluation compared with those in the normal control. OR slightly influenced lymphocyte proliferation, peritoneal macrophage phagocytosis, and immune organ indices in mice. Thus, 3.0 g/kg BW OR showed potential for the development of functional food with antioxidant and immune-enhancement activities.
DOI: 10.6084/m9.figshare.20039400
title: Preparation and evaluation of 2-methoxyestradiol-loaded pH-sensitive liposomes

description: The development and clinical application of 2-methoxyestradiol (2-ME) as a new type of antitumor drug are limited due to its poor solubility, rapid metabolism in vivo, and large oral dosage. 2-ME-loaded pH-sensitive liposomes (2-ME-PSLs) was prepared containing the lipids, Lipoid E-80 (E-80), cholesteryl hemisuccinate (CHEMS), and cholesterol (CHOL) via thin-film ultrasonic dispersion. First, preparation conditions of 2-ME-PSLs were optimized by orthogonal test. Then 2-ME-PSL was characterized, and the release behavior and stability of 2-ME-PSL in vitro were evaluated. The optimal preparation conditions for 2-ME-PSLs were as follows: 2-ME : E-80+CHEMS 1:15; CHOL : E-80+CHEMS 1:5; ultrasonication time 20 minutes. The mean particle size, PDI, zeta potential, and entrapment efficiency (EE) of 2-ME-PSLs were 116 ± 9 nm, 0.161 ± 0.025, −22.4 ± 1.7 mV, and 98.6 ± 0.5%, respectively. As viewed under a transmission electron microscope, 2-ME-PSLs were well dispersed and almost spherical. They exhibited significant pH-sensitive properties and were fairly stable when diluted with a physiological solution. In conclusion, 2-ME-PSLs were successfully prepared and possessed a favorable pH sensitivity and good dissolution stability with a normal solution.
DOI: 10.6084/m9.figshare.7980692.v1
title: Additional file 3: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S2. Gene information in four sub-clusters respectively. (XLS 8363 kb)
DOI: 10.6084/m9.figshare.7980725
title: Additional file 9: of RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.)

description: Table S5. The detailed information of each GO functional category of proteomic analysis. (XLS 1085 kb)
DOI: 10.6084/m9.figshare.20039195
title: Potential nutritional and antioxidant activity of various solvent extracts from leaves and stem bark of Anisophyllea laurina R. Br ex Sabine used in folk medicine

description: ABSTRACT Anisophyllea laurina is a plant that has been used in folk medicine to treat malaria, dysentery, diabetes, toothache and various skin diseases. Leaves extract had protein content of 9.68% and a high calcium content of 25084.317 mg/100 g while stem bark extract was found to contain greater amounts of calcium (8560.96 mg/100 g), potassium (7649.47 mg/100 g), magnesium (1462.49 mg/100 g) and iron (973.33 mg/100 g). Palmitic acid, linolenic acid, linoleic acid and oleic acid were the most abundant fatty acids in leaves and stem bark extracts. Furthermore, total phenolic (2382.39 mg GAE /100 g) and total flavonoid (385.79 mg QE/100 g) contents were abundant in stem bark while leaves extract was rich in total tannin content (3466.63 mg CE/100 g). However, both leaves and stem bark contained great amounts of vitamins and amino acids were a good source of antioxidant activities. For the individual polyphenol, stenophyllanin A (45.87 mg/g), casuarinin (24.55 mg/g) and digalloyl-HHDP-glucopyranose isomer (15.63 mg/g) were found to be the major compounds from the leaves whereas procyanidin tetramer (14.89 mg/g, (-)-Epicatechin (12.18 mg/g) and procyanidin trimer (11.25 mg/g) were the most predominant compounds from the stem bark. Additionally, the results revealed a significant and strong correlation between phenolic compounds and antioxidant activities.
DOI: 10.6084/m9.figshare.16893381.v1
title: Additional file 6 of Genome-wide association study reveals 14 new SNPs and confirms two structural variants highly associated with the horned/polled phenotype in goats

description: Additional file 6: Table S4: Genotypes and normalized copy numbers of both SVs in 74 domestic goats and 21 Bezoars analyzed by WGS.
DOI: 10.6084/m9.figshare.7981031.v1
title: MOESM13 of Construction and comprehensive analysis of a ceRNA network to reveal potential prognostic biomarkers for hepatocellular carcinoma

description: Additional file 13. R code.
DOI: 10.6084/m9.figshare.20039567
title: Evaluation of prevalence of different types of cancer and its chemotherapy in various ethnic groups of Pakistan: A retrospective study

description: The objective of the study was to evaluate different types of cancer and its chemotherapy in various ethnic groups of Pakistan. Ethnic groups includes, Pukhtoons, Punjabis, Sindhis, Muhajirs, Siraikis, Memoons, Hazaras, Hindkos, Baltis, Gilgitis, Kashmiris, and Afghanis. The data was collected from well reputed hospitals located in the different provinces of Pakistan. The collected data was taken from 15 hospitals where around 8500 patients visited during 2010 to 2017. From the visited patients, 8356 were analyzed for their ethnicity, age and sex while, 144 patients (male 77 and female 67) were excluded from analysis due to incomplete information or loss of follow-up. Among 8356 patients, 3762 were male (45%) whereas, 4594 were female (55%). The chemotherapy was carried out as per National Comprehensive Cancer Network guidelines (NCCN- guidelines). The most common five prevalent cancer among these ethnic groups were Head and Neck, Blood, Respiratory, Genito-urinary and Breast cancer. The most common cancer in female was breast cancer while, head and neck cancer was more prevalent in male. It can be concluded that the prevalence of cancer in Pakistan is very alarming, which may be due to lack of awareness, illiteracy, lack of national cancer control programs, and economics issues.
DOI: 10.6084/m9.figshare.20039195.v1
title: Potential nutritional and antioxidant activity of various solvent extracts from leaves and stem bark of Anisophyllea laurina R. Br ex Sabine used in folk medicine

description: ABSTRACT Anisophyllea laurina is a plant that has been used in folk medicine to treat malaria, dysentery, diabetes, toothache and various skin diseases. Leaves extract had protein content of 9.68% and a high calcium content of 25084.317 mg/100 g while stem bark extract was found to contain greater amounts of calcium (8560.96 mg/100 g), potassium (7649.47 mg/100 g), magnesium (1462.49 mg/100 g) and iron (973.33 mg/100 g). Palmitic acid, linolenic acid, linoleic acid and oleic acid were the most abundant fatty acids in leaves and stem bark extracts. Furthermore, total phenolic (2382.39 mg GAE /100 g) and total flavonoid (385.79 mg QE/100 g) contents were abundant in stem bark while leaves extract was rich in total tannin content (3466.63 mg CE/100 g). However, both leaves and stem bark contained great amounts of vitamins and amino acids were a good source of antioxidant activities. For the individual polyphenol, stenophyllanin A (45.87 mg/g), casuarinin (24.55 mg/g) and digalloyl-HHDP-glucopyranose isomer (15.63 mg/g) were found to be the major compounds from the leaves whereas procyanidin tetramer (14.89 mg/g, (-)-Epicatechin (12.18 mg/g) and procyanidin trimer (11.25 mg/g) were the most predominant compounds from the stem bark. Additionally, the results revealed a significant and strong correlation between phenolic compounds and antioxidant activities.
DOI: 10.6084/m9.figshare.20039244
title: The radiation protection role of heparin-SOD conjugate in irradiated mice

description: ABSTRACT Heparin-SOD conjugate (Hep-SOD) was prepared by modifying Cu,Zn-SOD with heparin. An acute radiation-induced mouse injury model was constructed to study the radiation protection effects of Hep-SOD conjugate. Fifty-six mice were randomly divided into seven groups: (I) normal control group; (II) irradiated control group; (III) positive control group (amifostine group, 300 mg/kg); (IV) SOD group (35000 U/kg); (V) high dosage of Hep-SOD group (70000 U/kg); (VI) medium dosage of Hep-SOD group (35000 U/kg); (VII) low dosage of Hep-SOD group (17500 U/kg). Drugs were intraperitoneally injected into each mouse 1 h before radiation except for the normal control group. All the irradiated groups were irradiated with 6 Gy. Organ indices, haematopoietic function indices, peripheral blood cells, liver function test, oxidative stress state and pathological observation were detected to study the effects of Hep-SOD on irradiated mice. Results showed that bone marrow suppression of irradiated mice could be reduced when treated by Hep-SOD before radiation. Oxidative stress detection and pathological observation of the liver and intestine showed that the damage caused by radiation was relieved when mice were treated with Hep-SOD before radiation. This study shows a new direction to prevent organisms from the damage caused by radiation.