DOI: 10.22002/mdfwm-c4s62
title: Caulobacter crescentus

description: wild type CB15N. 3rd bug of day, dual-axis dataset, lacy carbon. extended stationary phase, ATCC PYE formula, stressed cells
description: <strong>Tilt Series Date:</strong> 2004-06-24</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Dual Axis, tilt range: (-65.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection: D. Prabha Dias; processing: Bingni (Wen) Brunton</p>
<strong>Purification / Growth Conditions / Treatment:</strong> extended stationary phase, tapwater ATCC (PYE) media, wild-type CB15N</p>
<strong>Sample Preparation:</strong> Lacy carbon, 10nm gold on grids & in suspension, 100% humidity, -3 offset, 1 sec blot, 2 sec drain, gold on carbon & in cells</p>
  SAVE_Jun24t03comp.jpg: file associated to 3D Image #205 SAVE_Jun24t03p8.jpg: file associated to 3D Image #205 SAVE_Jun24t03s204.jpg: file associated to 3D Image #205 SAVE_Jun24t03s204b.jpg: file associated to 3D Image #205 pdd2004-06-24-3_slicer5002.jpg: Unknown pdd2004-06-24-3_slicer5006.jpg: Unknown ring pdd2004-06-24-3_slicer5007.jpg: Unknown pdd2004-06-24-3_slicer5010.jpg: Stacked membrane pdd2004-06-24-3_slicer5012.jpg: Unknown ring
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/rawdata/</p>CC04Jun24e100t03a.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/rawdata/CC04Jun24e100t03a.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC04Jun24e100t03b.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/rawdata/CC04Jun24e100t03b.mrc"
        > <i class="download icon"></i> Download </a></p>

        Dbyte.rec,  Reconstruction (Pixel Size 2.4 nm), 225.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/3dimage_203/Dbyte.rec"
        > <i class="download icon"></i> Download </a></p>

        Dbytea.rec,  Reconstruction (Pixel Size 2.4 nm), 734.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/3dimage_204/Dbytea.rec"
        > <i class="download icon"></i> Download </a></p>

        Dbyteb.rec,  Reconstruction (Pixel Size 2.4 nm) Notes for Full: Keywords:  .  extended stationary phase .  lacy carbon .  100 electrons per tilt series .  dual-axis .  CB15 .  GIFCCD, 880.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/3dimage_205/Dbyteb.rec"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jun24t03comp.tif,  file associated to 3D Image #205, 3.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/file_294/SAVE_Jun24t03comp.tif"
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        SAVE_Jun24t03p8.tif,  file associated to 3D Image #205, 1.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/file_296/SAVE_Jun24t03p8.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jun24t03s204.tif,  file associated to 3D Image #205, 649.0 kB  
        
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        SAVE_Jun24t03s204b.tif,  file associated to 3D Image #205, 362.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/file_300/SAVE_Jun24t03s204b.tif"
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        CC24Jun03a.mov,  file associated to 3D Image #205, 54.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-3/file_301/CC24Jun03a.mov"
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