cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/fmytdtk884
title: Design of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18

description: Primer pairs for the HR-HPV16 and 18 were designed to target the open reading frames (ORF) E6, E7, E1 and E2 in real-time PCR assays. This data is provided in the folders: Figures, Tables and HPV Alignments 

1. HPV alignments folder contain the following files: 
For gene-specific qPCR primer design, poorly conserved nucleotide regions were identified from HPV alignments of full-length genomes of types HPV16, 31, 35 and HPV 18, 45 and 59 in FASTA format. The HPV clusters are available: “HPV16_31_35_alignment.fa”; “HPV18_45_59_alignment.fa”. 

2. A list of qPCR primers proposed is presented in Table 1. A graphic representation of amplification strategy is shown in Figure 1. Briefly, primers that produce overlapping amplicons less than 600 bp, that passed the secondary structure tests, designed with annealing temperatures above 58°C, were selected for experimental evaluation. Calibration curves were generated using SYBR Green chemistry and serial dilutions of a DNA standard as template for each genotype evaluated. For ORF HPV18 E7, the amplification plots, including the melt curve analysis are shown in Figure 2. Primer pairs producing multiple products (Figure 3), are not recommended. Complete information for primer pair used in real-time PCR assays are described in Table 2. All this information is presented in Figures and Tables folders.
DOI: 10.17632/zrcxmy2257
title: Data for: Influence of Asphaltene on Wax Deposition: Deposition Inhibition and Sloughing

description: Figure 1- 13
DOI: 10.25438/wes02.28987853.v1
title: Zero Waste in U.S. Cities

description: The dataset contains data about the 132 U.S. Cities that signed the Cities Race to Zero pledge in 2024. It codes the following for each city: its publicly listed waste diversion rate, the year of that rate, its state, 2022 population, 2022 per capita income, ACEEE score, whether the state has a beverage bill, whether the state has a food waste diversion law, presence of an accredited university, strength of its zero waste plan, strength of its zero waste goals, the type of zero waste related staff person the city has, whether the city is a signatory to the Global Covenant of Mayors for Climate and Energy, whether the city is a member of C40, and whether the city is a member of ICLEI.
DOI: 10.6084/m9.figshare.12016989.v8
title: counts

description: <b>tblcounts_asv_melt.csv: A melted table containing</b><b>the sequence counts of each ASV detected in 12,546 stool samples</b> SampleID: stool sample identifier ASV: identifier of the ASVs Count: number of reads<b><br></b> <b>tblqpcr.csv: 16S rRNA qPCR data for 3,342 stool samples</b> SampleID: stool sample identifier qPCR16S: 16S copies per gram of stool sample<br><b>tblcounts_{asv,genus,family,order,class,phylum}_wide.csv: Wide-format taxa-by-sample table of counts at different taxonomic levels</b>
DOI: 10.6084/m9.figshare.19710568.v2
title: Antimicrobial resistance surveillance report, Mukdahan Hospital, Mukdahan, Thailand, 01 Jan 2020 to 31 Jan 2021

description: The AMASSplus application is an extension of AMASS Version 1.1 that was released on 12 January 2021. AMASSplus has an additional report (page 38-40) on selected notifiable bacterial diseases. For implementation of AMASSplus at your hospitals in Thailand.The application can be downloaded from https://www.amass.website This is a repository of the files for Mukdahan Hospital.The attached four files are: 1) The AMR surveillance report automatically generated from AMASSplus using hospital admission and microbiology data of Mukdahan Hospital in Thailand 2) Aggregated summary data in .csv format automatically generated from AMASSplus 3) Data dictionary for microbiology data file of Mukdahan Hospital (configured by the hospital to be used with the AMASSplus application) 4) Data dictionary for hospital admission data of Mukdahan Hospital (configured by the hospital to be used with the AMASSplus application) Please note that the two data dictionary files can be re-used with the data files of the hospital in the future in cases when variables names and values in the raw data remained unchanged.
DOI: 10.6084/m9.figshare.1352064.v28
title: Biology lessons for schools using the fruit fly Drosophila

description: OVERVIEW: An overview of resources given here is listen under this link: https://droso4schools.wordpress.com/learning-resources/<br>RATIONALE: For more than 100 years the fruit fly <em>Drosophila</em> <em>melanogaster</em> has been at the forefront of biological research and thus provides highly refined conceptual understanding of biology. Accordingly, much of the core biology curriculum can be explained extremely well using flies, spiced up with exciting, simple and inexpensive experiments that are memorable, reflect contemporary research and provide students with clear comprehension. Here we provide readily tested school biology lessons in form of PowerPoint files which are supported by explanatory background information, risk assessments, lesson plans, instructions and any other additional information that may save teachers time and effort to set up these lessons in their own classes. Furthermore, we set up the droso4schools website (link below) which provides further support materials suitable for revision or homework tasks, or simply to enhance the learning experience. Further <em>Drosophila</em>-specific information and resources can be found under the second link given below. If you find our resources helpful for your teaching, please, help us by sending an informal email to Andreas.Prokop@manchester.ac.uk<br>
DOI: 10.6084/m9.figshare.14277125.v1
title: Rapid detection of Mycobacterium tuberculosis in children using blood and urine specimens

description: Abstract INTRODUCTION: Laboratory and clinical features of childhood tuberculosis (TB) are non-specific and establishing an accurate diagnosis remains a challenge. This study evaluated a Single tube nested-PCR (STNPCR) to detect genomic DNA of Mycobacterium tuberculosis complex in blood and urine. METHODS: Biological samples were obtained from children (&lt;15 years old) with clinical suspicion of pulmonary and extrapulmonary TB at public hospitals in Recife-Pernambuco, Brazil. Cultures yielded negative results in a majority of childhood TB cases, which are generally paucibacillary. A set of clinical, epidemiological, radiological, and laboratory criteria with evident clinical improvement after anti-TB treatment were frequently used to define childhood TB cases. RESULTS: Ninety children with clinical suspicion were enrolled in this study (44 with TB and 46 without TB). The pulmonary TB group had 20 confirmed cases and 46 negative controls, while the extrapulmonary TB group had 24 confirmed cases. The STNPCR showed sensitivities to pulmonary and extrapulmonary TB of 47.4% and 52.2% (blood) and 38.8% and 20% (urine), respectively. Considering the low performance of STNPCR on separate samples, we decided to perform a combined analysis (parallel sensitivity analysis) of the results from blood and urine samples. The parallel sensitivity increased to 65% in blood and 62.5% in urine. The specificity in both samples ranged from 93.5-97.8%. CONCLUSIONS: Although STNPCR showed moderate sensitivity, the specificity is high; therefore, the test can be used as an auxiliary tool to diagnose TB in children. It is a rapid test that demonstrated better performance than other diagnostic tests in paucibacillary samples as it does in childhood tuberculosis.
DOI: 10.17632/gvmhn7znyg
title: Gamification and Xenophobia

description: Using an innovative and experimental methodology, this data represents the results of a study focuses on Islamophobia and intercultural competence among higher education students through a gamified learning intervention featuring six culturally themed challenges inspired by classic board games. 

Pre- and post-intervention questionnaires were administered to 325 university students enrolled in interculturality-related courses to assess their knowledge, learning preferences, and intercultural attitudes. A control group of 45 students, who followed a traditional lecture-based format, was also included to evaluate the effectiveness and replicability of the game-based approach.
DOI: 10.6084/m9.figshare.12610538.v1
title: PacBio full-length transcriptome of echolocating bats

description: PacBio full-length transcriptome of echolocating bats (constant-frequency and frequency-modulated bats).
DOI: 10.15468/dl.shzeph
title: Occurrence Download

description: A dataset containing 754 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "TaxonKey is Potentilla nivea L.",
 "Year is less than or equal to 2000",
 "Year is greater than or equal to 1970",
 {
 "or" : [
 {
 "not" : {
 "EstablishmentMeans" : [
 "is one of (MANAGED, INTRODUCED)"
 ]
 }
 },
 "EstablishmentMeans is null"
 ]
 },
 {
 "or" : [
 "CoordinateUncertaintyInMeters is less than 1000",
 "CoordinateUncertaintyInMeters is null"
 ]
 }
 ]
}

 The dataset includes 754 records from 72 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013368-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.17632/3snnp89967
title: Erbil Diabetes Dataset

description: This dataset presents diabetes data in Erbil, Kurdistan Region of Iraq. It was collected from individuals referred by expert physicians for diabetes-related tests, following suspicions of the disease. The tests were conducted by highly trained professionals in a private laboratory in Erbil. The dataset includes 14 features across 661 observations. The HbA1c feature can be used as a target variable. During the data collection process, all ethical guidelines and measures were strictly followed by both local and international regulations. Detailed descriptions of the features can be found in the readme file.

The features included in the dataset are as follows:
1. Social Life: Indicates whether the patient resides in an urban area (city) or a rural area (village).
2. Blood Pressure (BP): The patient’s blood pressure measurement.
3. Age: The age of the patient in years.
4. Sex: The gender of the patient (male or female).
5. Cholesterol Level (Chol): The total cholesterol level in the patient’s blood.
6. Triglycerides (Trig): The level of triglycerides in the patient’s blood.
7. High-Density Lipoprotein (HDL): The level of HDL cholesterol, often referred to as 'good' cholesterol.
8. Low-Density Lipoprotein (LDL): The level of LDL cholesterol, often referred to as 'bad' cholesterol.
9. Very Low-Density Lipoprotein (VLDL): The level of VLDL cholesterol, another type of 'bad' cholesterol.
10. Family Relationship (Genetics): Indicates the genetic predisposition to diabetes based on family history.
11. Glycated Hemoglobin (HbA1c): A measure of blood sugar levels over the past two to three months, which can be used as a target variable.
12. Body Mass Index (BMI): A measure of body fat based on height and weight.
13. Random Blood Sugar (RBS): The blood sugar level at a random time of day.
14. Fasting Blood Sugar (FBS): The blood sugar level after fasting, typically measured after not eating for at least 8 hours.
DOI: 10.6084/m9.figshare.1588553.v1
title: HuR antagonizes the effect of an intronic pyrimidine-rich sequence in regulating WT1 +/−KTS isoforms

description: ABSTRACTWT1 +KTS and -KTS isoforms only differ in three amino acids in protein sequence but show significant functional difference. The +/−KTS isoforms were generated by alternative usage of two adjacent 5′ splice sites at RNA level, however, how these two isoforms are regulated is still elusive. Here we report the identification of an intronic pyrimidine-rich sequence that is critical for the ratio of +/−KTS isoforms, deletion or partial replacement of the sequence led to full/significant shift to -KTS isoform. To identify trans-factors that can regulate +/−KTS isoforms via the binding to the element, we performed RNP assembly using in vitro transcribed RNA with or without the pyrimidine-rich sequence. Mass spectrometry analysis of purified RNPs showed that the element associated with many splicing factors. Co-transfection of these factors with WT1 reporter revealed that HuR promoted the production of -KTS isoform at the reporter level. RNA immuno-precipitation experiment indicated that HuR interacted with the pyrimidine-rich element in WT1 intron 9. We further presented evidence that transient or stable over-expression of HuR led to enhanced expression of endogenous -KTS isoform. Moreover, knockdown of HuR resulted in decreased expression of endogenous -KTS isoform in 293T, SW620, SNU-387 and AGS cell lines. Together, these data indicate that HuR binds to the pyrimidine-rich sequence and antagonize its effect in regulating WT1 +/−KTS isoforms.
DOI: 10.6084/m9.figshare.26341428.v1
title: Students’ academic culture: the mediating role of academic commitment in the relationship between academic resilience and academic performance of university students

description: Despite the fact that much research in higher education institutions has focused on students’ academic achievement from various perspectives, the roles of learners’ academic resilience and academic commitment in their academic performance have got little attention. Thus, the present study aimed at examining the role of students’ academic resilience on academic performance through the mediation role of academic commitment. A quantitative research approach, correlational research design, was used. A total of 243 undergraduate students were selected through simple random sampling, using computer-generated codes. Adapted self-report Likert scales and adapted questionnaires were used to collect data. For quality control, the collected data were entered twice into EpiData 4.6.0.6 before exported to SPSS AMOS software. A Structural Equation Model (SEM) was mainly applied to test the mediation analysis. The findings of the study revealed that undergraduate university students had moderate levels of academic commitment, academic resilience and academic performance. Moreover, academic commitment was discovered to have a partial mediating effect on the relationship between academic resilience and academic performance. Thus, the study concluded that learners’ academic commitment mediated their growing academic resilience and academic performance. In an era of evolving higher education landscapes, the pursuit of academic achievement remains a central focus. Nevertheless, the critical roles that learner academic commitment and resilience play in influencing academic performance have been underappreciated amidst the large corpus of research. This research delves into this uncharted territory, of undergraduate students which the findings light up a landscape where university students demonstrate moderate levels of academic resilience, academic performance, and academic commitment. These insights have profound implications for educators, policymakers, and institutions, posing a pathway to enhance educational strategies and support systems, ultimately fostering resilient, commitment and high-performing learners that met the demand of 21 century world.
DOI: 10.6084/m9.figshare.28714382.v1
title: Gameplay databases

description: sqlite databases of the gameplay from two cohorts of measurement
DOI: 10.6084/m9.figshare.25347760.v2
title: <b>Widespread crab burrows enhance methane emissions from coastal blue carbon ecosystems</b>

description: Investigation data on crab burrows (scale of large climate gradient, from China).The data includes：Particle size, environmental variables, soil organic carbon (SOC), soil organic matter (SOM), and light-heavy fraction organic carbon (L-HFOC) of 106 sediment samples (burrow or matrix).GHGs emission rate, GHGs cumulative flux, and enzyme activity of 34 sediment samples (incubation experiment).<br>
DOI: 10.6084/m9.figshare.3551799.v2
title: Data Paper. Data Paper

description: File List shapefiles.zip -- Shapefiles compressed together in a zipped dirctory. 33,505,926 bytes readme.txt -- 3 January 2012 explanation for file corrections <br> <br> All .csv files are ASCII text, comma separated:<br> allrecords_density.csv - 125,201 records, 9,414,808 bytes<br> allrecords_cover.csv - 81,365 records, 7,606,877 bytes<br> quad_info.csv - 44 records, 656 bytes.<br> quad_inventory.csv - 14 records, 2,086 bytes<br> species_list.csv - 155 records, 5,128 bytes<br> daily_climate_data.csv - 5114 records, 128,441 bytes<br> annuals_counts_v3.csv - 2,536 records, 68,899 bytes<br> species_name_changes.csv - Originally assigned plant names were corrected for synonyms based on the USDA Plants Database (http://plants.usda.gov/). Name changes are recorded in this file. 86 records, 3,387 bytes<br> DescriptionThis historical data set consists of 44 permanent 1-m<sup>2</sup> quadrats located on northern mixed prairie in Miles City, Montana, USA. Individual plants in these quadrats were identified and mapped annually from 1932 through 1945. Quadrats were located in six pastures assigned to cattle grazing treatments with light, moderate, and heavy stocking rates of 1.24, 0.92, and 0.76 ha/ animal-unit-month (two pastures in each). These data provide unique opportunities to test the interactive effects of grazing and climate variables on demographic rates, plant–plant interactions, and population and community dynamics. We provide the following data and data formats: (1) the digitized maps in shapefile format; (2) a tabular version of the entire data set (a table that replaces the full spatial data with one (<i>x</i>,<i>y</i>) coordinate for each individual perennial plant record); (3) grazing treatment information; (4) an inventory of the years each quadrat was sampled; (5) a species list, containing information on plant growth forms and shapefile geometry type (e.g., points or polygons); (6) a record of changes in species names; (7) daily precipitation and temperature records; and (8) counts of annual plants in the quadrats (annuals were counted, not mapped, by the original mappers).<br> <br> <i>Key words: climate; demography; Geographic Information Systems (GIS); Montana; northern mixed prairie; plant community; plant population; species interactions</i>
DOI: 10.6084/m9.figshare.6128225.v1
title: Historical data from the National Drug Code Directory

description: This dataset contains text file snapshots from the National Drug Code Directory during the years 2000-2018, as available in the Internet Archive (web.archive.org) on April 11, 2018. The files span several database and formatting changes, but together they provide a more comprehensive list of National Drug Codes than are available in the most recent database snapshot (https://www.fda.gov/Drugs/InformationOnDrugs/ucm142438.htm).
DOI: 10.6084/m9.figshare.16989442.v2
title: mdd2021asi

description: GWAS summary statistics from the Psychiatric Genomics Consortium (PGC)
DOI: 10.6084/m9.figshare.28616126.v1
title: supporting information

description: species information in feces and quadrats
DOI: 10.6084/m9.figshare.28911701.v1
title: DATA-EU Word.xlsx

description: The data targets 488 European companies across various sectors from 2014 to 2022.
DOI: 10.6084/m9.figshare.29099018.v2
title: Individual Data Points - In-vivo rat mechanical testing

description: Mechanical function of the rat plantar flexors were tested in vivo. The data displayed are maximum torque production, and the torque-angular velocity curves constructed from isotonic (constant load) contractions and isokinetic (constant velocity) contractions.
DOI: 10.6084/m9.figshare.24964686.v1
title: Pattern Recognition and Predictive Warning

description: Mechanical, Electrical and Thermal Information on Mechanical Abuse of Batteries under Different Operating Conditions
DOI: 10.6084/m9.figshare.27249645.v1
title: Antconc concordance analysis of the use of "Kami" and "Kita"

description: Antconc concordance analysis of the use of "Kami" and "Kita" in term of Clusivity.
DOI: 10.17632/dp4y36fjw5
title: SAFmaps - Sugarcane

description: Developed in the context of SAFmaps containing the georeferenced database and a case study report on SAF production based on the ATJ-SPK / ethanol (from sugarcane & corn) pathway. SAFmaps is a database platform with information on the potential of promising feedstock for the production of Sustainable Aviation Fuels (SAF) in Brazil. SAFmaps includes a database of different feedstocks, background information and assessment results.
DOI: 10.6084/m9.figshare.12820814.v1
title: Genome annotation of PacBio assemblies of Neurospora sitophila

description: This dataset contains genome annotation of 4 genome Neurospora sitophila assemblies generated from PacBio data. The annotation was generated using MAKER and was based on de novo gene inferrence and homology to N. crassa proteins and transcripts.<br>Strains:11356232W1426W1434<br>For more information, see Svedberg et al., (2020): https://www.biorxiv.org/content/10.1101/2020.01.29.923946v1<br>
DOI: 10.6084/m9.figshare.16725457.v2
title: Thibault et al_Fallow lands

description: Dataset for manuscript entitled: Afforestation of agricultural fallow lands for carbon sequestration: How does it compare with natural succession?Submitted to Plant and Soil
DOI: 10.6084/m9.figshare.26165890.v3
title: Source Data.xlsx

description: Improving thermal insulation is vital for addressing thermal protection and energy efficiency challenges. Though silica aerogel has a record-low thermal conductivity at ambient pressure, its high production cost, due to its nanoscale porous structure, has hindered its widespread use. In this study, we introduce a cost-effective and mild method that enhances insulation by incorporating phase change materials (PCMs) into a micron-porous framework. With a thermal conductivity at 0.041 W m-1K-1 on par with conventional insulation materials, this PCMs aerogel presents additional advantages for thermal protection from transient high-temperature loads by effectively delaying heat propagation through heat absorption. Moreover, the PCMs aerogel remains stable under cyclic deformation and heating up to 300 °C and is self-extinguishing in the presence of fire. Our approach offers a promising alternative for affordable insulation materials with potential wide applications in thermal protection and energy conservation areas.
DOI: 10.6084/m9.figshare.7900862.v1
title: Chemometric analysis of selective polyphenolic groups in <i>Asparagus racemosus</i> (Shatavar) root extracts by traditional and supercritical fluid (CO<sub>2</sub>) based extractions

description: <i>Asparagus racemosus</i> root extracts were prepared by supercritical fluid (CO<sub>2</sub>), soxhlet, and maceration-based methods also with various pretreatments. Thereafter, these root extracts were analyzed by High-Performance Liquid Chromatography, along with the chemometric study of the disparate phenolic groups. Among these, supercritical fluid (CO<sub>2</sub>) based extract has a larger number of polar compounds and the antioxidant activity (98.54 ± 0.22 <i>µ</i>M Trolox equivalent mg<sup>−1</sup>). It also has the best cell viability (94.37 ± 1.12%) and insulin release (0.82 ± 1.12 ng mL<sup>−1</sup>) on β-pancreatic RINm-5F cells whereas, the best extractive yield (75.80 ± 3.44% w/w) was observed for pretreated aqueous soxhlet-based extract.