DOI: 10.5061/dryad.sc1tm24
title: Data from: Which species, how many, and from where: Integrating habitat suitability, population genomics, and abundance estimates into species reintroduction planning

description: Extirpated organisms are reintroduced into their former ranges worldwide
 to combat species declines and biodiversity losses. The growing field of
 reintroduction biology provides guiding principles for reestablishing
 populations, though criticisms remain regarding limited integration of
 initial planning, modeling frameworks, interdisciplinary collaborations,
 and multi-species approaches. We used an interdisciplinary, multi-species,
 quantitative framework to plan reintroductions of three fish species into
 Abrams Creek, Great Smoky Mountains National Park, USA. We first assessed
 the appropriateness of habitat at reintroduction sites for banded sculpin
 (Cottus carolinae), greenside darter (Etheostoma blennioides), and mottled
 sculpin (Cottus bairdii) using species distribution modeling. Next, we
 evaluated the relative suitability of nine potential source stock sites
 using population genomics, abundance estimates, and multiple-criteria
 decision analysis (MCDA) based on known correlates of reintroduction
 success. Species distribution modeling identified mottled sculpin as a
 poor candidate, but banded sculpin and greenside darter as suitable
 candidates for reintroduction based on species-habitat relationships and
 habitats available in Abrams Creek. Genotyping by sequencing revealed
 acceptable levels of genetic diversity at all candidate source stock
 sites, identified population clusters, and allowed for estimating the
 number of fish that should be included in translocations. Finally, MCDA
 highlighted priorities among candidate source stock sites that were most
 likely to yield successful reintroductions based on differential
 weightings of habitat assessment, population genomics, and the number of
 fish available for translocation. Our integrative approach represents a
 unification of multiple recent advancements in the field of reintroduction
 biology and highlights the benefit of shifting away from simply choosing
 nearby populations for translocation to an information-based science with
 strong a priori planning coupled with several suggested posteriori
 monitoring objectives. Our framework can be applied to optimize
 reintroduction successes for a multitude of organisms and advances the
 science of reintroduction biology by simultaneously addressing a variety
 of past criticisms of the field.
description: Barcodes and
 adaptersIllumina_adapters_and_keyfile.xlsxMetadataAll_species_site_metadata.xlsxGBS reads lane 1This is 1 of 4 lanes sequenced on an Illumina NextSeq for this project. All sequencing details can be found in the published manuscript. The four lanes of sequencing data are accompanied by a two files: 1. barcodes and adapters 2. metadata1_AHJKKTBGXY_s_1_fastq.gzGBS reads lane 2This is 2 of 4 lanes sequenced on an Illumina NextSeq for this project. All sequencing details can be found in the published manuscript. The four lanes of sequencing data are accompanied by a two files: 1. barcodes and adapters 2. metadata2_AHJKKTBGXY_s_1_fastq.gzGBS reads lane 3This is 3 of 4 lanes sequenced on an Illumina NextSeq for this project. All sequencing details can be found in the published manuscript. The four lanes of sequencing data are accompanied by a two files: 1. barcodes and adapters 2. metadata3_AHJKKTBGXY_s_1_fastq.gzGBS reads lane 4This is 4 of 4 lanes sequenced on an Illumina NextSeq for this project. All sequencing details can be found in the published manuscript. The four lanes of sequencing data are accompanied by a two files: 1. barcodes and adapters 2. metadata4_AHJKKTBGXY_s_1_fastq.gz