DOI: 10.57760/sciencedb.36204
title: Raw and processed data for: CD36/CSF3 axis–mediated microglial polarization drives neurovascular unit dysfunction in retinal ischemia–reperfusion injury (OGD/R, Transwell co-culture)
title: Raw and processed data for: CD36/CSF3 axis–mediated microglial polarization drives neurovascular unit dysfunction in retinal ischemia–reperfusion injury (OGD/R, Transwell co-culture)

description: Raw and processed data for: CD36/CSF3 axis–mediated microglial polarization drives neurovascular unit dysfunction in retinal ischemia–reperfusion injury (OGD/R, Transwell co-culture) Version v1.0 Creators: You Zhou, First Affiliated Hospital of Kunming Medical University Corresponding / Data contact: Di Yang, First Affiliated Hospital of Kunming Medical University, Email: fionayangdi@outlook.com Funding This study was supported by Basic Research Program of Yunnan Province (202201AY070001-077 Di Yang, 202301AT070092 Di Yang), Yunnan Provincial Youth Talent Research Grant (RLQB20220002 Di Yang), 535 Talent Project of First Affiliated Hospital of Kunming Medical University (2023535D19 Di Yang) Associated manuscript Title: The detrimental impact of CD36/CSF3-mediated microglial polarization on neurovascular unit in retinal ischemia–reperfusion injury.     Study overview This dataset supports a study investigating whether CD36 upregulates CSF3 in retinal microglia under oxygen–glucose deprivation/reperfusion (OGD/R) and whether the CD36/CSF3 axis drives microglial polarization (M1/M2), thereby affecting retinal neurovascular unit (NVU) components. Experimental system: Cell types: primary mouse retinal microglia; primary mouse retinal microvascular endothelial cells (mRMECs); murine retinal ganglion cell line RGC-5. Injury model: OGD/R in microglia. Intervention: CD36 knockdown by shRNA plasmid; rescue by exogenous CSF3 (100 ng/mL). Co-culture: Transwell inserts to assess microglia-mediated effects on RGC-5 and mRMECs. Experimental groups (group codes) See 00_README/GROUP_CODES.tsv. Core groups used across assays: Control, OGD/R, OGD/R+shNC, OGD/R+shCD36, OGD/R+shCD36+CSF3 (100 ng/mL); Dose-finding groups for CSF3 on microglia apoptosis: Control,CSF3_10, CSF3_50, CSF3_100, CSF3_150. Data types included RAW data: ELISA plate reader exports and concentrations (pg/mL) calculated from standard curves (CSF3, TNF-α, IL-1β, TGF-β, IL-10); qPCR instrument exports (Ct), melt curves and 2^−ΔΔCt results; Western blot uncropped scans and processed data; Flow cytometry FCS files, images and Flow apoptosis summary tables (early/late apoptosis, total apoptosis); CCK-8 normalized viability; Microscopy images (immunofluorescence INOS,Arg1,TUJ1; scratch; invasion; tube formation) PROCESSED data and quantified microscopy metrics (migration rate, invaded cell count, tube formation metrics) Folder structure:00_README/: documentation, data dictionary, file manifest, experiment design, group codes, data dictionary  01_PROTOCOLS/: experimental protocols (PDF)02_RAW_DATA/: raw instrument outputs, images and processed/derived data tables used for statistics and plots03_FIGURES_FOR_PAPER/: figure-ready panels 04_SUPPLEMENTARY/: Construction of Mouse CD36 Interference Vector, RGC5 STR report and atlasFile naming convention Files follow: [Project]_[Assay]_[Cell]_[Group]_[TargetOrReadout]_[Rep]_[DateYYYYMMDD]_[Instrument]_[RAW|PROC]_v01.[ext]How to reproduce the main statistics Primary statistical analysis: One-way ANOVA (GraphPad Prism 9) with significance threshold p &lt; 0.05 . Ethics and compliance: No human participants or identifiable personal data are included. Primary mouse retinal microglia and mRMECs were commercially purchased; no animal procedures for cell isolation were performed, therefore no local animal ethics approval number is available. RGC-5 authentication: STR report included. RGC-5 STR authentication report is provided in 04_SUPPLEMENTARY/STR_RGC5_report. Data license: CC BY 4.0 
description: Raw and processed data for: CD36/CSF3 axis–mediated microglial polarization drives neurovascular unit dysfunction in retinal ischemia–reperfusion injury (OGD/R, Transwell co-culture) Version v1.0 Creators: You Zhou, First Affiliated Hospital of Kunming Medical University Corresponding / Data contact: Di Yang, First Affiliated Hospital of Kunming Medical University, Email: fionayangdi@outlook.com Funding This study was supported by Basic Research Program of Yunnan Province (202201AY070001-077 Di Yang, 202301AT070092 Di Yang), Yunnan Provincial Youth Talent Research Grant (RLQB20220002 Di Yang), 535 Talent Project of First Affiliated Hospital of Kunming Medical University (2023535D19 Di Yang) Associated manuscript Title: The detrimental impact of CD36/CSF3-mediated microglial polarization on neurovascular unit in retinal ischemia–reperfusion injury.     Study overview This dataset supports a study investigating whether CD36 upregulates CSF3 in retinal microglia under oxygen–glucose deprivation/reperfusion (OGD/R) and whether the CD36/CSF3 axis drives microglial polarization (M1/M2), thereby affecting retinal neurovascular unit (NVU) components. Experimental system: Cell types: primary mouse retinal microglia; primary mouse retinal microvascular endothelial cells (mRMECs); murine retinal ganglion cell line RGC-5. Injury model: OGD/R in microglia. Intervention: CD36 knockdown by shRNA plasmid; rescue by exogenous CSF3 (100 ng/mL). Co-culture: Transwell inserts to assess microglia-mediated effects on RGC-5 and mRMECs. Experimental groups (group codes) See 00_README/GROUP_CODES.tsv. Core groups used across assays: Control, OGD/R, OGD/R+shNC, OGD/R+shCD36, OGD/R+shCD36+CSF3 (100 ng/mL); Dose-finding groups for CSF3 on microglia apoptosis: Control,CSF3_10, CSF3_50, CSF3_100, CSF3_150. Data types included RAW data: ELISA plate reader exports and concentrations (pg/mL) calculated from standard curves (CSF3, TNF-α, IL-1β, TGF-β, IL-10); qPCR instrument exports (Ct), melt curves and 2^−ΔΔCt results; Western blot uncropped scans and processed data; Flow cytometry FCS files, images and Flow apoptosis summary tables (early/late apoptosis, total apoptosis); CCK-8 normalized viability; Microscopy images (immunofluorescence INOS,Arg1,TUJ1; scratch; invasion; tube formation) PROCESSED data and quantified microscopy metrics (migration rate, invaded cell count, tube formation metrics) Folder structure:00_README/: documentation, data dictionary, file manifest, experiment design, group codes, data dictionary  01_PROTOCOLS/: experimental protocols (PDF)02_RAW_DATA/: raw instrument outputs, images and processed/derived data tables used for statistics and plots03_FIGURES_FOR_PAPER/: figure-ready panels 04_SUPPLEMENTARY/: Construction of Mouse CD36 Interference Vector, RGC5 STR report and atlasFile naming convention Files follow: [Project]_[Assay]_[Cell]_[Group]_[TargetOrReadout]_[Rep]_[DateYYYYMMDD]_[Instrument]_[RAW|PROC]_v01.[ext]How to reproduce the main statistics Primary statistical analysis: One-way ANOVA (GraphPad Prism 9) with significance threshold p &lt; 0.05 . Ethics and compliance: No human participants or identifiable personal data are included. Primary mouse retinal microglia and mRMECs were commercially purchased; no animal procedures for cell isolation were performed, therefore no local animal ethics approval number is available. RGC-5 authentication: STR report included. RGC-5 STR authentication report is provided in 04_SUPPLEMENTARY/STR_RGC5_report. Data license: CC BY 4.0