DOI: 10.5061/dryad.c2fqz61nn
title: Dataset: Spatiotemporal patterns of salmon winter habitat usage in the Northeast Pacific uncovered by environmental DNA

description: Amplicon sequence variant (asv) dataset from multi amplicon metabarcoding
 environmental DNA analysis from the International Year of the Salmon
 Signature expedition to the Northeast Pacific in Feb/Mar 2022. A table of
 all asv generated in the study is provided  in ASV.csv, broken
 down by amplicon and sample and listing the occurance of said asv in said
 sample. Further, total reads per amplicon/sample bin are recorded as well
 as a taxonomic assignment. Note that this data is provided prior to
 filtering, e.g. "Homo sapiens" reads are abundant and should be
 considered contamination. The metadata to sampling stations is provided in
 the sample_metadata.csv which contains the date, lat/long, as well as
 detailed environmental metadata associated with the sample. Refer to Deeg
 et al. "Spatiotemporal patterns of salmon winter habitat usage in the
 Northeast Pacific uncovered by environmental DNA" ICES Journal of
 Marine Science 2025 (https://doi.org/10.1093/icesjms/fsaf104) for
 detailed information on sample collection, sequencing and bioinformatic
 processing.
description: # Dataset: Spatiotemporal patterns of salmon winter habitat usage in the
 Northeast Pacific uncovered by environmental DNA Dataset DOI:
 10.5061/dryad.c2fqz61nn ## **Files and variables** File: ASV.csv
 Variables:  * sample_id(_tech_rep): Sample ID to match the metadata.
 "_A" , "_B" suffix denotes technical replicates * asv:
 Amplicon sequence variant sequence * occurrence_count: Number of
 occurrences of asv in sample * forward_primer: Forward primer used to
 amplify the amplicon * reverse_primer: Reward primer used to amplify the
 amplicon * taxon: Operational taxonomic unit assigned to the asv *
 reads_amplicon_sample: Total number of reads generated in sequencing for
 this amplicon and sample combination File: sample_metadata.csv Variables:
 * sample_ID: Sample ID * vessel: Sampling vessel * doy: Day of year 2022 *
 method: Collection method * method_detail:  Collection method detail *
 collection_system: Collection method system * filt_sys: Filtration system
 * sample_depth_m: Sampling depth * latitude: Latitude * longitude:
 Longitude * filt_material: Material of filtration membrane * filter_type:
 Filter type * filter_pore_size_u: Filter pore size in micron *
 cruise_program: Cruise program * sst: Sea surface temperature (C); NA
 values due to cloud cover obstructing satellite observations or for
 control samples * ssta: Sea surface temperature anomaly (K); NA values due
 to cloud cover obstructing satellite observations or for control samples *
 sla_sla: Sea level anomaly (m); NA values due to cloud cover obstructing
 satellite observations or for control samples * sla_u: Currents: absolute
 geostrophic velocity : Zonal Component, m s-1; NA values due to cloud
 cover obstructing satellite observations or for control samples *
 sla_v: Currents: absolute geostrophic velocity : Meridian Component, m
 s-1; NA values due to cloud cover obstructing satellite observations or
 for control samples * chl_prod: Productivity (Net Primary Productivity Of
 Carbon, mg C m-2 day-1); NA values due to cloud cover obstructing
 satellite observations or for control samples * sla_eke: Eddy Kinetical
 Energy, m2 s-2; NA values due to cloud cover obstructing satellite
 observations or for control samples * is_field_control: Sample is a field
 control * is_lab_control: Sample is a lab control ### Access information
 Data was derived from the following sources:
 [https://coastwatch.pfeg.noaa.gov/erddap/griddap/jplMURSST42.html](https://coastwatch.pfeg.noaa.gov/erddap/griddap/jplMURSST42.html) [https://coastwatch.pfeg.noaa.gov/erddap/griddap/nesdisSSH1day.html](https://coastwatch.pfeg.noaa.gov/erddap/griddap/nesdisSSH1day.html) [https://coastwatch.pfeg.noaa.gov/erddap/griddap/productivity_viirs_snpp_daily.html](https://coastwatch.pfeg.noaa.gov/erddap/griddap/productivity_viirs_snpp_daily.html)