DOI: 10.5061/dryad.rr4xgxd7k
title: Data from: One, two or three? Integrative species delimitation of short-range endemic Hemicycla species (Gastropoda: Helicidae) from the Canary Islands based on morphology, barcoding, AFLP and ddRADseq data

description: Hemicycla mascaensis and H. diegoi are short-range endemics that occur
 allopatrically in small areas in the Teno Mountains in the western part of
 Tenerife (Canary Islands). Both taxa have been recognised as distinct
 species based on differences in shell morphology and genital anatomy.
 Preliminary molecular analyses using mitochondrial markers suggested a
 potential paraphyly of H. diegoi with regard to H. mascaensis. We here use
 multilocus AFLP data and ddRADseq data as well as distribution data, data
 on shell morphology and genital anatomy to assess the status of these taxa
 using phylogenetic analyses, species tree reconstruction, and molecular
 species delimitation based on the multispecies coalescent as implemented
 in BFD* and BPP in an integrative approach. Our analyses show that, based
 on the analysis of multilocus data, the two taxa are reciprocally
 monophyletic. Species delimitation methods, however, tend to recognise all
 investigated populations as distinct species, albeit neither lending
 unambiguous support to any of the species hypothesis. The comparison of
 the anatomy of distal genital organs further suggests differentiation
 within H. mascaensis. This highlights the need for a balanced weighting of
 arguments from different lines of evidence to determine species status and
 calls for cautious interpretations of the results of molecular species
 delimitation analyses, especially in organisms with low active dispersal
 capacities and expected distinct population structuring such as land
 snails. Taking all available evidence into account, we favour to recognise
 H. mascaensis and H. diegoi as distinct species, acknowledging, though,
 that the recognition of both taxa as subspecies (with possibly a third yet
 undescribed) would also be an option as morphological differentiation is
 within the limits of other land snail species that are traditionally
 subdivided into subspecies.
description: # Data from: One, two or three? Integrative species delimitation of
 short-range endemic Hemicycla species (Gastropoda: Helicidae) from the
 Canary Islands based on morphology, barcoding, AFLP and ddRADseq data
 [https://doi.org/10.5061/dryad.rr4xgxd7k](https://doi.org/10.5061/dryad.rr4xgxd7k) ## Description of the data and file structure ### Files and variables #### File: Data\_set\_01\_Hemicycla\_COI1\_16S.nexus **Description:** Aligned partial sequences of the mitochondrial cytochrome c oxidase subunit 1 (*cox1*) (alignment positions 1-655) and 16S rRNA (alignment positions 656-1,488) genes in nexus format. #### File: Data\_set\_02\_AFLP.nex **Description:** AFLP loci obtained from 15 specimens belonging to the genus *Hemicycla* in nexus format. #### File: Data\_set\_03\_AFLPred.nex **Description:** AFLP loci obtained from 9 specimens belonging to the *Hemicycla* *mascaensis* complex in nexus format. #### File: Data\_set\_4\_RADseq\_SNP\_all\_polymorphic\_positions.nex **Description:** Concatenated sequences of 5,454 ddRAD loci with a total of 61,809 SNPs (IUPAC-coded) obtained from 11 specimens belonging to the genus *Hemicycla* in nexus format. #### File: Data\_set\_5\_RADseq\_SNPs\_one\_random\_bialleic\_SNP\_per\_locus.nex **Description:** Data set in nexus format including one bialleci SNP per locus selected randomly from the 5,454 loci in Data Set 4. #### File: Data\_set\_6\_RADseq\_SNPS\_one\_randam\_biallelic\_per\_locus\_present\_in\_all\_pops\_mascaensis\_snapp.nexus **Description:** Data set in nexus format including one bialleci SNP per locus selected randomly from 15,297 ddRAD loci obtained from 7 specimens belonging to the *Hemicycla* *mascaensis* complex. #### File: Data\_set\_7\_all\_loci\_alleles.fas **Description:** Sequence data for 5,482 ddRAD loci obtained from 11 specimens belonging to the genus *Hemicycla* in fasta format. #### File: Data\_set\_8\_BPP.txt **Description:** Data set including 300 randomly selected, polymorphic loci from Data set 7 obtained for specimens belonging to the *H*. *mascaensis* complex in BPP input format.