DOI: 10.5061/dryad.4tmpg4fnv
title: Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 1

description: This dataset contains raw whole-genome sequencing data (FASTQ files) for
 wild-type and riboflavin biosynthetic gene deletion mutants of
 Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains
 were generated to investigate the source of metabolic antigens recognized
 by mucosal-associated invariant T (MAIT) cells, which detect intermediates
 from the riboflavin biosynthetic pathway. To confirm successful gene
 deletions and rule out off-target effects, whole-genome sequencing was
 performed for each mutant and its corresponding wild-type strain. This
 dataset supports the verification of targeted genetic modifications and
 enables comparative genomic analyses related to MR1 antigen production in
 mycobacteria.
description: # Whole genome sequence data of Mycobacterium tuberculosis and
 Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic
 pathway- Part 1 Dataset DOI:
 [10.5061/dryad.4tmpg4fnv](10.5061/dryad.4tmpg4fnv) ## Description of the
 data and file structure Whole Genome Sequence of wild type strain and
 riboflavin biosynthetic pathway mutants of *Mycobacterium tuberculosis*
 (Mtb) and *Mycolicibacterium smegmatis* (Msm). Also, this dataset has part
 2 which is available at https://doi.org/10.5061/dryad.vq83bk454 Naming
 convention for files:  1. Lab name 2. Strain Name 3. Sample Number 4.
 Batch Number 5. Read (Forward/Reverse) 6. Replicate e.g. For file name
 "Dobos_msmegriba2comp_7_S25_L002_R2_001": Lab name: Dobos;
 Strain Name: msmegriba2comp_7; Sample Number: S25; Batch Number: L002;
 Read: R2 (Reverse Read); Replicate: 001 ### Files and variables #### File:
 Dobos\_mtbribcko\_11\_S29\_L002\_R2\_001.fastq **Description**: Reverse
 Read for Mtb *ΔribC* #### File:
 Dobos\_msmegriba2comp\_7\_S25\_L002\_R2\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribG::ribG* #### File:
 D0bos\_msmegribgcomp\_5\_S23\_L002\_R2\_001.fastq.gz
 **Description:** Reverse Read for Msm *ΔribG::ribG* #### File:
 Dobos\_msmegribccomp\_1\_S19\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribC::ribC* #### File:
 Dobos\_msmegribgko\_6\_S24\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribG* #### File:
 Dobos\_msmegribgko\_6\_S24\_L002\_R2\_001.fastq.gz
 **Description:** Reverse Read for Msm *ΔribG* #### File:
 Dobos\_msmegwt\_9\_S27\_L002\_R2\_001.fastq.gz **Description:** Reverse
 Read for Msm Wild Type #### File:
 Dobos\_msmegwt\_9\_S27\_L002\_R1\_001.fastq.gz **Description:**  Forward
 Read for Msm Wild Type #### File:
 Dobos\_mtbriba2ko\_17\_S35\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Mtb *ΔribA2* #### File:
 Dobos\_mtbriba2ko\_17\_S35\_L002\_R2\_001.fastq.gz
 **Description:** Reverse Read for Mtb *ΔribA2* #### File:
 Dobos\_msmegribhko\_4\_S22\_L002\_R2\_001.fastq.gz
 \**Description: **Reverse Read for Msm *ΔribH* #### File:
 Dobos\_msmegribhko\_4\_S22\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribH* #### File:
 Dobos\_msmegriba2comp\_7\_S25\_L002\_R1\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribA2::ribA2* #### File:
 Dobos\_msmegribccomp\_1\_S19\_L002\_R2\_001.fastq.gz
 **Description:** Forward Read for Msm *ΔribC::ribC* #### File:
 Dobos\_mtbriba2comp\_16\_S34\_L002\_R1\_001.fastq.gz
 **Description:** Reverse Read for Mtb *ΔribA2::ribA2* #### File:
 Dobos\_mtbriba2comp\_16\_S34\_L002\_R2\_001.fastq.gz **Description:** 
 Reverse Read for Mtb *ΔribA2::ribA2* #### File:
 Dobos\_msmegribcko\_2\_S20\_L002\_R1\_001.fastq.gz
 **Description:**  Forward Read for Msm *ΔribC* #### File:
 Dobos\_msmegribcko\_2\_S20\_L002\_R2\_001.fastq.gz
 **Description:**  Reverse Read for Msm *ΔribC* #### File:
 Dobos\_mtbribccomp10\_S28\_L002\_R1\_001.fastq.gz
 **Description:**  Forward Read for Mtb *ΔribC::ribC* #### File:
 Dobos\_msmegribhcomp\_3\_S21\_L002\_R1\_001.fastq.gz
 **Description:**  Forward Read for Msm *ΔribA2::ribA2* #### File:
 Dobos\_msmegribhcomp\_3\_S21\_L002\_R2\_001.fastq.gz
 **Description:**  Reverse Read for Msm *ΔribH::ribH* #### File:
 D0bos\_msmegribgcomp\_5\_S23\_L002\_R1\_001.fastq.gz
 **Description:**  Forward Read for Msm *ΔribG::ribG* #### File:
 Dobos\_mtbribccomp10\_S28\_L002\_R2\_001.fastq.gz
 **Description:**  Forward Read for Mtb *ΔribC::ribC* #### File:
 Dobos\_mtbribcko\_11\_S29\_L002\_R1\_001.fastq.gz
 **Description:**  Forward Read for Mtb *ΔribC*