cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11930133
title: Cognitive-behavioral therapy for treatment-resistant depression in adults and adolescents: a systematic review

description: Abstract Objective To conduct a systematic review of literature on use and efficacy of cognitive-behavioral therapy (CBT) for treatment of treatment-resistant depression in adults and adolescents. Methods We performed a systematic review according to the Prisma Guidelines of literature indexed on the PubMed, SciELO, Psychiatry Online, Scopus, PsycArticles, Science Direct and the Journal of Medical Case Reports databases. Randomized controlled trials, open studies and case reports were included in the review. Results The searches returned a total of 1,580 articles, published from 1985 to 2017. After applying the inclusion criteria, 17 articles were selected, their complete texts were read and 8 were included in this review. Four of these studies were randomized controlled trials with adults, one of which covered a post-study follow-up period; two were randomized controlled trials with adolescents, one of which presented follow-up data; one was an open study; and one was a case report. The studies provide good quality and robust evidence on the topic addressed. Conclusions A combination of CBT with pharmacotherapy for treatment-resistant patients shows a decrease in depressive symptoms. CBT can be an effective type of therapy for adults and adolescents with treatment-resistant depression.
DOI: 10.6084/m9.figshare.11930136
title: New Detector Based on Composite of Carbon Nanotubes with Nanoparticles of Cobalt Oxide for Carbohydrates Analysis by HPLC with Reverse Pulsed Amperometric Detection

description: This work aims to present an alternative detector for the determination of arabinose, galactose, glucose and xylose in hydrolyzed sugarcane bagasse. The detector was developed using an electrode modified with multi-walled carbon nanotubes containing cobalt oxide nanoparticles (GCE/MWCNT/CoOOH) applied in high-performance liquid chromatography (HPLC) with reverse pulsed amperometric detection (RPAD). The limits of detection obtained were 3.4 × 10-6, 4.4 × 10-6, 3.6 × 10-6 and 5.0 × 10-6 mol L-1 for arabinose, galactose, glucose and xylose, respectively. The standard addition method was used to determine the concentration of sugars in the hydrolyzed sugarcane. The determined concentrations for arabinose, galactose, glucose, and xylose were 1.0 × 10-3, 7.2 × 10-5, 3.0 × 10-3 and 2.4 × 10-3 mol L-1, respectively. The results demonstrate that this new method can be used for the detection of sugars without the interference of other electroactive species.
DOI: 10.6084/m9.figshare.11930133.v1
title: Cognitive-behavioral therapy for treatment-resistant depression in adults and adolescents: a systematic review

description: Abstract Objective To conduct a systematic review of literature on use and efficacy of cognitive-behavioral therapy (CBT) for treatment of treatment-resistant depression in adults and adolescents. Methods We performed a systematic review according to the Prisma Guidelines of literature indexed on the PubMed, SciELO, Psychiatry Online, Scopus, PsycArticles, Science Direct and the Journal of Medical Case Reports databases. Randomized controlled trials, open studies and case reports were included in the review. Results The searches returned a total of 1,580 articles, published from 1985 to 2017. After applying the inclusion criteria, 17 articles were selected, their complete texts were read and 8 were included in this review. Four of these studies were randomized controlled trials with adults, one of which covered a post-study follow-up period; two were randomized controlled trials with adolescents, one of which presented follow-up data; one was an open study; and one was a case report. The studies provide good quality and robust evidence on the topic addressed. Conclusions A combination of CBT with pharmacotherapy for treatment-resistant patients shows a decrease in depressive symptoms. CBT can be an effective type of therapy for adults and adolescents with treatment-resistant depression.
DOI: 10.6084/m9.figshare.11930136.v1
title: New Detector Based on Composite of Carbon Nanotubes with Nanoparticles of Cobalt Oxide for Carbohydrates Analysis by HPLC with Reverse Pulsed Amperometric Detection

description: This work aims to present an alternative detector for the determination of arabinose, galactose, glucose and xylose in hydrolyzed sugarcane bagasse. The detector was developed using an electrode modified with multi-walled carbon nanotubes containing cobalt oxide nanoparticles (GCE/MWCNT/CoOOH) applied in high-performance liquid chromatography (HPLC) with reverse pulsed amperometric detection (RPAD). The limits of detection obtained were 3.4 × 10-6, 4.4 × 10-6, 3.6 × 10-6 and 5.0 × 10-6 mol L-1 for arabinose, galactose, glucose and xylose, respectively. The standard addition method was used to determine the concentration of sugars in the hydrolyzed sugarcane. The determined concentrations for arabinose, galactose, glucose, and xylose were 1.0 × 10-3, 7.2 × 10-5, 3.0 × 10-3 and 2.4 × 10-3 mol L-1, respectively. The results demonstrate that this new method can be used for the detection of sugars without the interference of other electroactive species.
DOI: 10.6084/m9.figshare.11930124
title: Analysis of a sudden doubling of the space between walls of an infinite 1-dim square well: a surprising result at first

description: Abstract In this work, we would like to point out how an usually recommended exercise in Quantum Mechanics textbooks can lead to a surprising result at first, and how the clarification of this surprising result can help to reinforce ideas of Quantum Mechanics. We consider the problem of a particle in an infinite square well which suddenly has its width doubled. The surprise arrives in the fact that different results can be obtained whether the well is considered non-symmetrical or symmetrical, even though both are physically identical, as it is well-known.
DOI: 10.6084/m9.figshare.11930127
title: TEACHERS IN DICTATORSHIP AND DEMOCRACY: BIOGRAPHICAL PERSPECTIVE OF TEACHERS AT THE END OF A PROFESSIONAL CAREER

description: ABSTRACT This paper analyzes the professional trajectories of five teachers at two important moments in the history of Chile: dictatorship and the return to democracy. We rely on the biographical method in order to understand, from the voices of the leading figures, their significant experiences. These were analyzed according to the Grounded Theory precepts. The results describe a career path marked by fear and distrust. We conclude that the last years of work reveal discouragement with the educational changes that structure the loss of autonomy and collaboration. The teachers analyze the permanent search for standardized results and experience a deep disappointment with the democratic regime installed at the end of the Pinochet dictatorship. We assume that these aspects organize the identity of the teacher at the end of the career and also encourage a form of suffering.
DOI: 10.6084/m9.figshare.11930130.v1
title: Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair

description: Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.
DOI: 10.6084/m9.figshare.11930121
title: SSD - a free software for designing multimeric mono-, bi- and trivalent shRNAs

description: Abstract RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a dose-dependent manner, when compared to the control group. Thus, SSD software allows the easy design of multimeric shRNAs, for single or multiple simultaneous knockdowns, which is especially interesting for studies involving large amounts of double-stranded molecules.
DOI: 10.6084/m9.figshare.11930124.v1
title: Analysis of a sudden doubling of the space between walls of an infinite 1-dim square well: a surprising result at first

description: Abstract In this work, we would like to point out how an usually recommended exercise in Quantum Mechanics textbooks can lead to a surprising result at first, and how the clarification of this surprising result can help to reinforce ideas of Quantum Mechanics. We consider the problem of a particle in an infinite square well which suddenly has its width doubled. The surprise arrives in the fact that different results can be obtained whether the well is considered non-symmetrical or symmetrical, even though both are physically identical, as it is well-known.
DOI: 10.6084/m9.figshare.11930127.v1
title: TEACHERS IN DICTATORSHIP AND DEMOCRACY: BIOGRAPHICAL PERSPECTIVE OF TEACHERS AT THE END OF A PROFESSIONAL CAREER

description: ABSTRACT This paper analyzes the professional trajectories of five teachers at two important moments in the history of Chile: dictatorship and the return to democracy. We rely on the biographical method in order to understand, from the voices of the leading figures, their significant experiences. These were analyzed according to the Grounded Theory precepts. The results describe a career path marked by fear and distrust. We conclude that the last years of work reveal discouragement with the educational changes that structure the loss of autonomy and collaboration. The teachers analyze the permanent search for standardized results and experience a deep disappointment with the democratic regime installed at the end of the Pinochet dictatorship. We assume that these aspects organize the identity of the teacher at the end of the career and also encourage a form of suffering.
DOI: 10.6084/m9.figshare.11930115
title: Maternal transmission of mitochondrial diseases

description: Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In addition, poorly understood mechanisms act in the female germline to prevent the accumulation of deleterious mtDNA in the following generations. In this review, we outline basic aspects of mitochondrial inheritance in mammals and how they may lead to maternally-inherited diseases. Furthermore, we discuss potential therapeutic strategies for these diseases, which may be used in the future to prevent their transmission.
DOI: 10.6084/m9.figshare.11930121.v1
title: SSD - a free software for designing multimeric mono-, bi- and trivalent shRNAs

description: Abstract RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a dose-dependent manner, when compared to the control group. Thus, SSD software allows the easy design of multimeric shRNAs, for single or multiple simultaneous knockdowns, which is especially interesting for studies involving large amounts of double-stranded molecules.
DOI: 10.6084/m9.figshare.11930115.v1
title: Maternal transmission of mitochondrial diseases

description: Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In addition, poorly understood mechanisms act in the female germline to prevent the accumulation of deleterious mtDNA in the following generations. In this review, we outline basic aspects of mitochondrial inheritance in mammals and how they may lead to maternally-inherited diseases. Furthermore, we discuss potential therapeutic strategies for these diseases, which may be used in the future to prevent their transmission.
DOI: 10.6084/m9.figshare.11930118
title: Yield of onion in soil with cattle manure and nitrogen

description: ABSTRACT Onion is the third vegetable crop in economic importance for Brazil, only behind potato and tomato, and responds with increased production to the use of organic and mineral fertilizers. The objective of this study was to evaluate the effect of doses of cattle manure and nitrogen (N) on onion growth and yield. The experimental design used was randomized blocks, in 6 x 2 factorial scheme, corresponding to six doses of cattle manure (0; 10; 20; 30; 40 and 50 t ha-1), in the presence and absence of N, with four repetitions. The variables studied were: shoot fresh and dry masses, total and marketable bulb yields, percentage of bulbs classified and leaf N content. There was an interactive effect (p ≤ 0.05) of cattle manure and N doses on shoot fresh and dry masses, total and marketable yields, classification of bulbs according to transverse diameter and N concentration in onion bulbs. The optimal dose for marketable yield of bulbs is 27 t ha-1 of cattle manure in the presence of N.
DOI: 10.6084/m9.figshare.11930109
title: Kinetics of Lumefantrine Thermal Decomposition Employing Isoconversional Models and Artificial Neural Network

description: Thermal analysis can be used to determine shelf-life and kinetic parameters in pharmaceutical systems. This work investigates the kinetic of lumefantrine thermal decomposition, an antimalarial, using non-isothermal and isothermal experimental data. The non-isothermal conditions are analyzed applying Vyazovkin method, while isothermal conditions employ models fitting procedure and artificial neural network. Lumefantrine was characterized by powder X-ray diffraction and Fourier transform infrared spectroscopy. The initial stage of lumefantrine thermal decomposition, about 5% of conversion, corresponds to the loss of chlorine and hydroxyl, being correctly predicted by the neural network as a complex event. At room temperature, the D3 model is appropriate to describe the process, once the half-life time is 19 months, in agreement with manufacturer. Isoconversional model determined the activation energy along the whole process while isothermal methodology determined the global value considering the entire process. The results provide important information for the pharmaceutical industry to assay levels of acceptable lumefantrine contents.
DOI: 10.6084/m9.figshare.11930112
title: Roles of the mitochondrial replisome in mitochondrial DNA deletion formation

description: Abstract Mitochondrial DNA (mtDNA) deletions are a common cause of human mitochondrial diseases. Mutations in the genes encoding components of the mitochondrial replisome, such as DNA polymerase gamma (Pol γ) and the mtDNA helicase Twinkle, have been associated with the accumulation of such deletions and the development of pathological conditions in humans. Recently, we demonstrated that changes in the level of wild-type Twinkle promote mtDNA deletions, which implies that not only mutations in, but also dysregulation of the stoichiometry between the replisome components is potentially pathogenic. The mechanism(s) by which alterations to the replisome function generate mtDNA deletions is(are) currently under debate. It is commonly accepted that stalling of the replication fork at sites likely to form secondary structures precedes the deletion formation. The secondary structural elements can be bypassed by the replication-slippage mechanism. Otherwise, stalling of the replication fork can generate single- and double-strand breaks, which can be repaired through recombination leading to the elimination of segments between the recombination sites. Here, we discuss aberrances of the replisome in the context of the two debated outcomes, and suggest new mechanistic explanations based on replication restart and template switching that could account for all the deletion types reported for patients.
DOI: 10.6084/m9.figshare.11930112.v1
title: Roles of the mitochondrial replisome in mitochondrial DNA deletion formation

description: Abstract Mitochondrial DNA (mtDNA) deletions are a common cause of human mitochondrial diseases. Mutations in the genes encoding components of the mitochondrial replisome, such as DNA polymerase gamma (Pol γ) and the mtDNA helicase Twinkle, have been associated with the accumulation of such deletions and the development of pathological conditions in humans. Recently, we demonstrated that changes in the level of wild-type Twinkle promote mtDNA deletions, which implies that not only mutations in, but also dysregulation of the stoichiometry between the replisome components is potentially pathogenic. The mechanism(s) by which alterations to the replisome function generate mtDNA deletions is(are) currently under debate. It is commonly accepted that stalling of the replication fork at sites likely to form secondary structures precedes the deletion formation. The secondary structural elements can be bypassed by the replication-slippage mechanism. Otherwise, stalling of the replication fork can generate single- and double-strand breaks, which can be repaired through recombination leading to the elimination of segments between the recombination sites. Here, we discuss aberrances of the replisome in the context of the two debated outcomes, and suggest new mechanistic explanations based on replication restart and template switching that could account for all the deletion types reported for patients.
DOI: 10.6084/m9.figshare.11930118.v1
title: Yield of onion in soil with cattle manure and nitrogen

description: ABSTRACT Onion is the third vegetable crop in economic importance for Brazil, only behind potato and tomato, and responds with increased production to the use of organic and mineral fertilizers. The objective of this study was to evaluate the effect of doses of cattle manure and nitrogen (N) on onion growth and yield. The experimental design used was randomized blocks, in 6 x 2 factorial scheme, corresponding to six doses of cattle manure (0; 10; 20; 30; 40 and 50 t ha-1), in the presence and absence of N, with four repetitions. The variables studied were: shoot fresh and dry masses, total and marketable bulb yields, percentage of bulbs classified and leaf N content. There was an interactive effect (p ≤ 0.05) of cattle manure and N doses on shoot fresh and dry masses, total and marketable yields, classification of bulbs according to transverse diameter and N concentration in onion bulbs. The optimal dose for marketable yield of bulbs is 27 t ha-1 of cattle manure in the presence of N.
DOI: 10.6084/m9.figshare.11930103
title: Glandular odontogenic cyst: report of an unusual case in the posterior mandible

description: ABSTRACT The glandular odontogenic cyst (GOC) is an uncommon developmental cyst that presents glandular differentiation and has potential for recurrence. Clinically, it is asymptomatic and it has slow growing. This report proposes to describe a clinical case of GOC diagnosed at the Service of Pathological Anatomy of a university in the northeast of Brazil, in the posterior region of the mandible, which was treated with mandibular resection and bone graft with iliac crest. One may conclude that clinical, microscopic and radiographic parameters were crucial tools for treatment choice.
DOI: 10.6084/m9.figshare.11930106
title: A comparative study of urban occupational structures: Brazil and United States

description: Abstract This paper compares the occupational structure of cities in Brazil and United States aiming to evaluate the extent to which the economic structure of these urban agglomerations is associated with the different stages of development, specifically when comparing a rich country with a developing one. Using a harmonized occupational database and microdata from the Brazilian 2010 Demographic Census and the U.S. American Community Survey (2008-2012), results show that Brazilian cities have a stronger connection between population size, both with occupational structure and human capital distribution, than the one found for cities in the United States. These findings suggest a stronger primacy of large cities in Brazil’s urban network and a more unequal distribution of economic activity across cities when compared to USA, indicating a strong correlation between development and occupational structure.