cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/9fpj3-m8t32
title: Cos7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A+VPS4DN constructs. Sample prepared for immunoEM using anti-CHM4A
description: <strong>Tilt Series Date:</strong> 2012-02-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -7.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4DN constructs provided by Wes Sundquist. Cell transfection, immunoEM, cryo-preservation and imaging done at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-20-1/3dimage_68763/</p>12feb20_4ADNimmunoEM_AT2BT1_0002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 462.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-20-1/3dimage_68763/12feb20_4ADNimmunoEM_AT2BT1_0002_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb20_4ADNimmunoEM_AT2BT1_0002.st,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-20-1/rawdata/12feb20_4ADNimmunoEM_AT2BT1_0002.st"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zygyr-4sp60
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C123
description: <strong>Tilt Series Date:</strong> 2012-03-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-36/3dimage_69164/</p>12mar28_pnlDN_C123_1_AT7BT1_0006_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-36/3dimage_69164/12mar28_pnlDN_C123_1_AT7BT1_0006_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12mar28_pnlDN_C123_1_AT7BT1_0006.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-36/rawdata/12mar28_pnlDN_C123_1_AT7BT1_0006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8hgt5-nv343
title: CLEM of TRIM5a-YFP in HeLa cells

description: Correlated light electron microscopy. Concentration of red 200 nm beads 1/300. 10 nm gold. Manual blotting. Cells grown on grids for 2 days and look vesiculated
description: <strong>Tilt Series Date:</strong> 2014-07-09</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 153.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Tom Hope and Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Gold London finder grids glow discharged twice. Concentration of red 200 nm beads 1/300 on grid. 10 nm gold. Manual blotting. Cells grown on grids for 2 days and look vesiculated.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-07-09-5/rawdata/</p>tomography5.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-07-09-5/rawdata/tomography5.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography5.rec,  Reconstruction (Pixel Size 1.56 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-07-09-5/3dimage_44532/tomography5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xyq2k-97769
title: Bsubtilis_ponA_sacculi

description: Bsubtilis_sacculi_ponA
description: <strong>Tilt Series Date:</strong> 2012-02-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-36/rawdata/</p>try2MJsacculi0009.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-36/rawdata/try2MJsacculi0009.mrc"
        > <i class="download icon"></i> Download </a></p>

        try2MJsacculi0009_part82_3.rec,  Reconstruction (Pixel Size 1.9 nm), 877.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-36/3dimage_26195/try2MJsacculi0009_part82_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y7svd-3sf61
title: HeLa cells

description: Looking for ESCRT near midbodies
description: <strong>Tilt Series Date:</strong> 2014-12-08</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> with Catherine Ullman and Doug mckay</p>
<strong>Sample Preparation:</strong> siRNA NUP 153+Thymidine for 14h,</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-12-08-7/rawdata/</p>midbody20005.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-12-08-7/rawdata/midbody20005.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody20005_part121_2.rec,  Reconstruction (Pixel Size 2.4 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-12-08-7/3dimage_48643/midbody20005_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/seej2-64959
title: tar-CF-EEEE on vesicles

description: Tar-CF, EEEE, vesicles
description: <strong>Tilt Series Date:</strong> 2014-01-02</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Lynmarie Thompson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Tar-CF-EEEE assembled on vesicles</p>
  aba2014-01-02-6_slicer9531.jpg: Escherichia coli .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-02-6/rawdata/</p>Vesicle4E02Jan0007.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-02-6/rawdata/Vesicle4E02Jan0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        Vesicle4E02Jan0007_full.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-02-6/3dimage_37290/Vesicle4E02Jan0007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/975h7-reg53
title: Pseudoalteromonas Iuteoviolacea MP129

description: P Iuteo DvipABhcpI Dbact2 whole cells from liquid culture
description: <strong>Tilt Series Date:</strong> 2013-07-12</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Pseudoalteromonas luteoviolacea</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: 10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> purified and processed by Gregor, Collaboration with Newman lab, taken at Janelia Farms</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid culture</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, Yi-Wei1</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-29/rawdata/</p>2013_07_12_JF_MP129_b_0024.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-29/rawdata/2013_07_12_JF_MP129_b_0024.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013_07_12_JF_MP129_b_0024_2k_secs_full.rec,  Reconstruction (Pixel Size 1.68 nm), 775.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2013-07-12-29/3dimage_28971/2013_07_12_JF_MP129_b_0024_2k_secs_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9qtza-9rd81
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-5/3dimage_69368/</p>12may29_C135_1_MEFnomitoDNA_AT1BT30006_part122_38.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-5/3dimage_69368/12may29_C135_1_MEFnomitoDNA_AT1BT30006_part122_38.rec"
        > <i class="download icon"></i> Download </a></p>

        12may29_C135_1_MEFnomitoDNA_AT1BT30006.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-5/rawdata/12may29_C135_1_MEFnomitoDNA_AT1BT30006.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/caxzh-4qa62
title: HIV capsid purified trim5 alpha

description: 41000 6df, with slit 91/A2
description: <strong>Tilt Series Date:</strong> 2013-12-13</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 91.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Purified recombinant trim5 alpha decorated with decorated HIV cores</p>
<strong>Sample Preparation:</strong> B grid</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-38/rawdata/</p>Trim5alpha41000df60018.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-38/rawdata/Trim5alpha41000df60018.mrc"
        > <i class="download icon"></i> Download </a></p>

        Trim5alpha41000df60018.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 711.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-12-13-38/3dimage_47834/Trim5alpha41000df60018.sirt.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qnw3r-7cb03
title: UU 2803- A413T delta P1P2

description: E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-30</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo, IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB medium, lysed with penicillin for 1 hour</p>
<strong>Sample Preparation:</strong> plunge frozen in R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-35/rawdata/</p>2803atlas10036.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-35/rawdata/2803atlas10036.mrc"
        > <i class="download icon"></i> Download </a></p>

        2803atlas10036_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-30-35/3dimage_20567/2803atlas10036_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2jjwg-z1v53
title: Agro pJW58

description: wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter.  Kanamycin-resistant (150 to 300 ug/mL)
description: <strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-2/rawdata/</p>Agro_pJW580002.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-2/rawdata/Agro_pJW580002.mrc"
        > <i class="download icon"></i> Download </a></p>

        Agro_pJW580002_part121_13.rec,  Reconstruction (Pixel Size 1.6 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-2/3dimage_39678/Agro_pJW580002_part121_13.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/svb0a-z5338
title: Agro VirB8-GFP

description: Raw data files of Agro VirB8-GFP
description: <strong>Tilt Series Date:</strong> 2013-07-08</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown for induction of T-pili at 19C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-08-14/rawdata/</p>VirB8_Titan0014.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-08-14/rawdata/VirB8_Titan0014.mrc"
        > <i class="download icon"></i> Download </a></p>

        VirB8_Titan0014_part121_78.rec,  Reconstruction (Pixel Size 1.68 nm), 516.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-08-14/3dimage_28136/VirB8_Titan0014_part121_78.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/aqw9t-bc507
title: Hylemonella gracilis

description: Raw data files of Hylemonella gracilis
description: <strong>Tilt Series Date:</strong> 2012-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 75.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-27/rawdata/</p>20120923_Hylemonella_10011.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
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        20120923_Hylemonella_10011_full.rec,  Reconstruction (Pixel Size 1.96 nm), 441.7 MB  
        
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