cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.996m7p
title: Occurrence Download

description: A dataset containing 10229 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is Xeno-canto - Bird sounds from around the world",
 "Country is Belgium"
 ]
}

 The dataset includes 10229 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003178-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.8mzrsc
title: Occurrence Download

description: A dataset containing 103 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Asia",
 "Country is China",
 "OccurrenceStatus is Present",
 "TaxonKey is Tsuga chinensis",
 "Year 1999-2025"
 ]
}

 The dataset includes 103 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003181-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.zbugtx
title: Occurrence Download

description: A dataset containing 47 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Petunia atkinsiana",
 "Country is Romania",
 "HasCoordinate is true"
 ]
}

 The dataset includes 47 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003183-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.16942307
title: SUPPLEMENTAL MATERIAL - Sex differences in stress response in the marine copepod, Calanus finmarchicus

description: Supplement material linked to the article " Sex differences in stress response in the marine copepods, Calanus finmarchicus.
DOI: 10.5281/zenodo.16942308
title: SUPPLEMENTAL MATERIAL - Sex differences in stress response in the marine copepod, Calanus finmarchicus

description: Supplement material linked to the article " Sex differences in stress response in the marine copepods, Calanus finmarchicus.
DOI: 10.15468/dl.fxfymd
title: Occurrence Download

description: A dataset containing 171 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Hypselecara temporalis)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 171 records from 21 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003182-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/12894005
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.15468/dl.esqdbu
title: Occurrence Download

description: A dataset containing 28993725 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Colias croceus, Lycaena tityrus, Ochlodes sylvanus, Nymphalidae, Satyrium ilicis, Plebejus idas, Cupido argiades, Phengaris alcon, Spialia sertorius, Euchloe crameri, Colias hyale, Hamearis lucina, Argynnis pandora, Melitaea parthenoides, Leptotes pirithous, Melitaea didyma, Lysandra bellargus, Limenitis populi)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "BasisOfRecord is Human Observation",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2025",
 {
 "or" : [
 "CoordinateUncertaintyInMeters is less than 5000",
 "CoordinateUncertaintyInMeters is null"
 ]
 },
 "Geometry POLYGON ((-180 -89.99893, 180 -89.99893, 180 83.59961, -180 83.59961, -180 -89.99893))"
 ]
}

 The dataset includes 28993725 records from 6321 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003142-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.r2ypcs
title: Occurrence Download

description: A dataset containing 4262 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Afrixalus aureus, Afrixalus delicatus, Afrixalus fornasini, Afrixalus knysnae, Afrixalus spinifrons, Amietia delalandii, Amietia fuscigula, Amietia hymenopus, Amietia poyntoni, Amietia vandijki, Amietia vertebralis, Anhydrophryne hewitti, Anhydrophryne ngongoniensis, Anhydrophryne rattrayi, Arthroleptella atermina, Arthroleptella bicolor, Arthroleptella draconella, Arthroleptella drewesii, Arthroleptella kogelbergensis, Arthroleptella landdrosia, Arthroleptella lightfooti, Arthroleptella rugosa, Arthroleptella subvoce, Arthroleptella villiersi, Arthroleptis stenodactylus, Arthroleptis wageri, Arthroleptis wahlbergii, Breviceps acutirostris, Breviceps adspersus, Breviceps bagginsi, Breviceps branchi, Breviceps carruthersi, Breviceps fuscus, Breviceps gibbosus, Breviceps macrops, Breviceps montanus, Breviceps mossambicus, Breviceps namaquensis, Breviceps passmorei, Breviceps pentheri, Breviceps rosei, Breviceps sopranus, Breviceps sylvestris, Breviceps verrucosus, Cacosternum aggestum, Cacosternum australis, Cacosternum boettgeri, Cacosternum capense, Cacosternum karooicum, Cacosternum namaquense, Cacosternum nanogularum, Cacosternum nanum, Cacosternum parvum, Cacosternum platys, Cacosternum rhythmum, Cacosternum striatum, Cacosternum thorini, Capensibufo deceptus, Capensibufo magistratus, Capensibufo rosei, Capensibufo selenophos, Capensibufo tradouwi, Chiromantis xerampelina, Hadromophryne natalensis, Heleophryne depressa, Heleophryne hewitti, Heleophryne orientalis, Heleophryne purcelli, Heleophryne regis, Heleophryne rosei, Hemisus guineensis, Hemisus guttatus, Hemisus marmoratus, Hildebrandtia ornata, Hyperolius argus, Hyperolius horstockii, Hyperolius marmoratus, Hyperolius pickersgilli, Hyperolius poweri, Hyperolius pusillus, Hyperolius semidiscus, Hyperolius tuberilinguis, Kassina senegalensis, Leptopelis mossambicus, Leptopelis natalensis, Leptopelis xenodactylus, Microbatrachella capensis, Natalobatrachus bonebergi, Phlyctimantis maculatus, Phrynobatrachus acridoides, Phrynobatrachus mababiensis, Phrynobatrachus natalensis, Phrynomantis annectens, Phrynomantis bifasciatus, Poyntonia paludicola, Poyntonophrynus fenoulheti, Poyntonophrynus vertebralis, Ptychadena anchietae, Ptychadena mossambica, Ptychadena nilotica)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((32.064 -28.9416,32.4847 -28.5786,30.5701 -28.5786,30.5701 -30.7329,30.6252 -30.6659,31.4179 -29.4257,31.8178 -29.0299,32.064 -28.9416))"
 ]
}

 The dataset includes 4262 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0050231-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.erpfjk
title: Occurrence Download

description: A dataset containing 80 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Wisteria sinensis",
 "Country is New Zealand",
 "HasCoordinate is true"
 ]
}

 The dataset includes 80 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0050233-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.16964995
title: Week-Hourly vehicle activity profiles for Germany based on BASt measurements

description: This dataset contains data from permanent automatic counting stations on highways and federal roads in Germany, collected by the German Federal Highway Research Institut (BASt). The raw data from the BASt's website (CC BY 4.0 licensed) has been aggregated to weekday-hour profiles for KFW and PKW vehicle types.
DOI: 10.5281/zenodo.16965042
title: Week-Hourly vehicle activity profiles for Germany based on BASt measurements

description: This dataset contains data from permanent automatic counting stations on highways and federal roads in Germany, collected by the German Federal Highway Research Institut (BASt). The raw data from the BASt's website (CC BY 4.0 licensed) has been aggregated to weekday-hour profiles for KFW and PKW vehicle types.
DOI: 10.15468/dl.7pbc7v
title: Occurrence Download

description: A dataset containing 14 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Wisteria sinensis",
 "Country is Netherlands",
 "HasCoordinate is true"
 ]
}

 The dataset includes 14 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0050232-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.x8c9t8
title: Occurrence Download

description: A dataset containing 11 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Occurrence evidence, Observation)",
 "TaxonKey is one of (Lepus americanus Erxleben, 1777)",
 "Country is one of (Åland Islands, Albania, Andorra, Austria, Belarus, Belgium, Bosnia and Herzegovina, Bulgaria, Croatia, Czechia, Denmark, Estonia, Faroe Islands, Finland, France, Germany, Gibraltar, Greece, Guernsey, Holy See, Hungary, Iceland, Ireland, Isle of Man, Italy, Jersey, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, Moldova, Republic of, Monaco, Montenegro, Netherlands, North Macedonia, Norway, Poland, Portugal, Romania, Russian Federation, San Marino, Serbia, Slovakia, Slovenia, Spain, Svalbard and Jan Mayen, Sweden, Switzerland, Ukraine, United Kingdom of Great Britain and Northern Ireland)"
 ]
}

 The dataset includes 11 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0021367-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7ebxc3
title: Occurrence Download

description: A dataset containing 1514 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Chiroptera",
 "Geometry POLYGON ((-95.11705427 16.21141454, -95.11705427 17.05107999, -94.34977377 17.05107999, -94.34977377 16.21141454, -95.11705427 16.21141454))",
 "Year is greater than or equal to 1900",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil)"
 ]
 }
 }
 ]
}

 The dataset includes 1514 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0021368-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/3gcbc-pgf04
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-08-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Take the sedimented cell clumps.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-32/3dimage_73149/</p>20150810_AK20_20032_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 14.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-32/3dimage_73149/20150810_AK20_20032_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150810_AK20_20032.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-08-10-32/rawdata/20150810_AK20_20032.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z3m0p-ra582
title: V. cholerae JAS288 delta-tcpQ

description: Classical strain with ctxA deletionStrain name JAS288 (tcpQ gene knonkout)Cell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2016-09-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 145.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Gabriela Kovacikova</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2016-09-14-18_slicer17072.jpg: DNA bundle?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-14-18/rawdata/</p>20160914_YWC216_10018.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-14-18/rawdata/20160914_YWC216_10018.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160914_YWC216_10018.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-09-14-18/3dimage_79089/20160914_YWC216_10018.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zde8b-4th83
title: Thermococcus koakarensis

description: Imaging Thermococcus koakarensis flagella
description: <strong>Tilt Series Date:</strong> 2016-09-20</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard Tk medium with starch and sulfur</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
  dga2016-09-20-13_slicer17084.jpg: Thermococcus kodakarensis  .  but may be a contaminant ? .   .  flagellum ?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-20-13/rawdata/</p>atlas10012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-20-13/rawdata/atlas10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10012_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-20-13/3dimage_79349/atlas10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/183bn-wjd04
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-04-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-09-21/rawdata/</p>20150409_AK005_10021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-09-21/rawdata/20150409_AK005_10021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150409_AK005_10021_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-09-21/3dimage_53797/20150409_AK005_10021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dz7ea-h8j32
title: Lp02-fixed

description: WT Lp02 cells were fixed with GA and re-suspended in  PBS.
description: <strong>Tilt Series Date:</strong> 2017-11-21</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Shuman Lab</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-21-58/rawdata/</p>Legionella_fixed_20171121a0050.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-21-58/rawdata/Legionella_fixed_20171121a0050.mrc"
        > <i class="download icon"></i> Download </a></p>

        Legionella_fixed_20171121a0050.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-21-58/3dimage_90516/Legionella_fixed_20171121a0050.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cwemn-tth66
title: V. cholerae RT4236 (stationary)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-07-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Take the sedimented cell clumps.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-27-15/3dimage_73232/</p>20150727_AK16_10015_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 14.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-27-15/3dimage_73232/20150727_AK16_10015_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150727_AK16_10015.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-07-27-15/rawdata/20150727_AK16_10015.mrc"
        > <i class="download icon"></i> Download </a></p>