DOI: 10.5061/dryad.0k6djhbbb
title: The physiological changes and the impact of rapid cold hardening on the survival ability of Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) under cold stress

description: Dastarcus helophoroides (Fairmaire) serves as an ideal biological control
 agent for forest beetles. However, its survival ability at low
 temperatures is a critical factor for its use in colder regions. This
 study investigates the survival ability, supercooling point (SCP), impact
 of rapid cold hardening (RCH), and physiological changes of D.
 helophoroides under cold stress. SCPs of adults that underwent RCH were
 lower than those of adults cultured at room temperature, with SCPs of
 -25.1°C and -16.9°C, respectively. As the cold stress temperature
 decreased in the test, the survival ability of D. helophoroides adults
 also diminished, reaching the lowest survival rates at a -20°C across all
 observation time points in the test. However, adults induced by RCH
 exhibited higher survival rates than those cultured at room temperature,
 suggesting that RCH enhances the cold resistance of D. helophoroides. The
 activities of superoxide dismutase (SOD) and catalase (CAT) in the adults
 increased as the stress temperature decreased. With prolonged cold stress,
 the activity of lactate dehydrogenase (LDH) initially decreased and then
 increased. Both fat and water content decreased with the lowering of
 stress temperature. These results provide insights into the cold
 resistance strategies of D. helophoroides.
description: # The physiological changes and the impact of rapid cold hardening on the
 survival ability of Dastarcus helophoroides (Fairmaire) (Coleoptera:
 Bothrideridae) under cold stress
 [https://doi.org/10.5061/dryad.0k6djhbbb](https://doi.org/10.5061/dryad.0k6djhbbb) ## Description of the data and file structure The survival rate, SOD activity, CAT activity, LDH activity, total fat, and water content data of *Dastarcus helophoroides* under cold stress were collected. ### Files and variables #### File: Enzyme\_activity.csv **Description:** This data file includes the activities of superoxide dismutase (SOD), catalase (CAT) and lactate dehydrogenase (LDH) of *Dastarcus helophoroides* under cold stress. ##### Variables * Temperature: Experimental samples treated temperature (  0℃, -5℃, -10℃, -15℃, and -20℃). * Group number: 3 groups were tested for each temperature and processing day.  * Processing days: Experimental samples treated days (1, 3, 5, 7, and 9 days). * SOD activity (mg pro/ml): superoxide dismutase activity (mg pro/ml). * CAT activity (U/ml): catalase activity (U/ml). * LDH activity (U/ml): lactate dehydrogenase activity (U/ml). #### File: Survival\_rate\_under\_cold\_stress\_data.csv **Description:** This data file includes the survival rate of regular *D. helophoroides* adults and rapid cold hardening *D. helophoroides* adults under cold stress. ##### Variables * Temperature: Experimental samples treated temperature (25℃, 0℃, -5℃, -10℃, -15℃, and -20℃). * Group number: 3 groups were tested for each temperature and processing day. * Processing days: Experimental samples treated days (1, 3, 5, 7, and 9 days). * RD survival rate (%): regular *D. helophoroides* survival rate (%) * RCHD survival rate (%):  rapid cold hardening *D. helophoroides* survival rate (%) #### File: Super\_cooling\_point\_data\_.csv **Description:** This data file includes the super cooling points of regular *D. helophoroides* adults and rapid cold hardening treated *D. helophoroides* adults. ##### Variables * Group number: 5 groups were tested for super cooling point * RD SCP (℃): regular *D. helophoroides* super cooling point * RCHD SCP (℃): rapid cold hardening treated *D. helophoroides* super cooling point #### File: Total\_fat\_and\_water\_content\_data.csv **Description:** This data file includes the total fat and water content of *D. helophoroides* adults under cold stress. ##### Variables * Temperature: Experimental samples treated temperature (25℃, 5℃, 0℃, -5℃, -10℃, -15℃, and -20℃). * Group number: 3 groups were tested for each temperature. * Processing days: Each sample were processing for 2 days. * Total fat (%): The total fat of *D. helophoroides* adults under cold stress. * Water content (%): The water content of *D. helophoroides* adults under cold stress.