DOI: 10.22002/9jb3m-5pq12
title: NR1769pKM101conjNR1769

description: Conjugation on 2% agar plate for 1h at 37C between skinny E. coli NR1769 having the F plasmid pKM101 and skinny E. coli NR1769
description: <strong>Tilt Series Date:</strong> 2014-10-24</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> NR1769 pKM101</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christian Baron made the strain with the plasmid.</p>
<strong>Sample Preparation:</strong> Vitrobot Mark IV</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-24-19/rawdata/</p>NR1760_pk101_conj0006.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
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        NR1760_pk101_conj0006_full.rec,  Reconstruction (Pixel Size 0.0 nm), 833.9 MB  
        
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        slicer000.tif,  3.0 MB  
        
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        slicer001.tif,  3.0 MB  
        
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        slicer002.tif,  3.0 MB  
        
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        slicer003.tif,  3.0 MB  
        
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        slicer004.tif,  3.0 MB  
        
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        slicer005.tif,  3.0 MB  
        
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        slicer006.tif,  3.0 MB  
        
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