DOI: 10.5061/dryad.q2bvq83ww
title: Data from: Dual oxidase is essential for molting, hatching and feeding in the brown planthopper

description: Dual oxidase (Duox) is well-known for its role in immunity and tyrosine
 crosslinking activity across various biological processes from mammals to
 holometabolous insects. Nevertheless, its function in hemimetabolous
 insects remains poorly understood. In this study, we explored the
 physiological roles of the Duox gene in a hemimetabolous insect, the brown
 planthopper. We conducted series of experiments, this dataset is the
 experimental data derived from our manuscript, including qRT-PCR data,
 survival rate data, hydrogen peroxide content data, body weight and length
 data, feeding behavior related data, and reproduction related data.
description: For qRT-PCR data, relative transcript levels were calculated
 using the 2<sup>−ΔΔCt</sup> method. All data were expressed as
 mean ± SD, based on at least three replicates. Statistical analyses were
 performed using GraphPad Prism 8.0 (GraphPad Software, CA, USA).
 Comparisons between two treatments were conducted using Student’s
 <em>t</em> test or Welch’s <em>t</em> test.
 Multiple comparisons were analyzed using one-way ANOVA with Games-Howell
 <em>post hoc</em> tests for non-homogeneous variances.
 Statistical significance was set at <em>p</em> &lt; 0.05
 (*) and <em>p</em> &lt; 0.01 (**).
description: # Dual oxidase is essential for molting, hatching and feeding in the brown
 planthopper Dataset DOI:
 [10.5061/dryad.q2bvq83ww](10.5061/dryad.q2bvq83ww) ## Description of the
 data and file structure We conducted series of experiments，this dataset is
 the experimental data derived from our manuscript, including qRT-PCR data
 (Temporal/Tissue-specific/Ovarian/RNAi efficiency), survival rate data,
 hydrogen peroxide content data, body weight and length data, feeding
 behavior related data, Vc treatment and hydrogen peroxide rescue data, and
 reproduction related data. ### Files and variables #### File:
 5th\_instar\_H2O2\_measurement.csv **Description:** Hydrogen peroxide
 level was measured 84 h after dsRNA-injection or at 0/48/84 h 5th-instar
 nymphs. ##### Variables * H2O2 level (μmol/g)  #### File:
 Body\_length\_and\_weight.csv **Description:** Body length and weight of
 females were measured.  ##### Variables * length(mm) * weight(mg) ####
 File: EPG\_and\_salivary\_sheath.csv **Description:** The EPG results are
 divided into four stages, and the data shown are the time proportions of
 each stage. Number of salivary sheaths from day1 to day3 were calculated.
 ##### Variables * EPG: NP/PP/N4/N5 * Salivary sheathes: d1/d2/d3 ####
 File: Ovarian\_expression.csv **Description:** qRT-PCR result of Duox gene
 expression in the ovary. ##### Variables * Day2-Day5 #### File:
 Reproduction\_related\_data.csv **Description:** Eggs number and
 hatchability of 15 females after dsRNA-injection. ##### Variables * Number
 of eggs * Hatchability(%) #### File: RNAi\_efficiency.csv
 **Description:** The RNAi efficiency was detected through qRT-PCR
 experiments. ##### Variables * RNAi efficiency of 3rd-5th instar nymphs *
 RNAi efficiency of egg * RNAi efficiency of ovary #### File:
 Survival\_rate.csv **Description:** The survival rates of 3rd-5th instar
 nymphs after RNAi. ##### Variables * 3rd-5th #### File:
 Temporal\_expression.csv **Description:** The temporal expression was
 detected through qRT-PCR experiments. ##### Variables * egg/1st-5th
 instar/Adult female/Adult male #### File: Tissue\_specific\_expression.csv
 **Description:** The tissue-specific expression was detected through
 qRT-PCR experiments. ##### Variables * Integument (Int), fat body (FB),
 gut (G), ovary (O), salivary gland (SG), and brain (B), and testis (T)
 #### File: VC\_treatment\_and\_H2O2\_rescue.csv **Description:**  Survival
 rates of vitamin C-feeding nymphs. Newly molted 5th-instar nymphs were fed
 an artificial diet supplemented with 25–100 mg/mL of vitamin C. H2O2
 levels in vitamin C-fed nymphs were measured. Newly molted 5th-instar
 nymphs were injected with dsRNA and then injected with 20 nmol H2O2 or PBS
 12 h before eclosion. ##### Variables      VC-treatment * Control *
 25mg/mL * 50mg/mL * 100mg/mL      H2O2-injection * dsGFP+PBS *
 dsDuox+H2O2（20nmol） * dsDuox+PBS ## Code/software All data are saved in
 csv files and can be viewed through excel or other software capable of
 viewing csv files.