DOI: 10.22002/7t5yr-ybt16
title: Caulobacter crescentus

description: wild type CB15N. 1st bug of day, dual-axis dataset, lacy carbon. extended stationary phase, ATCC PYE formula, stressed cells, crystalline feature, paired thick filament
description: <strong>Tilt Series Date:</strong> 2004-07-30</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Dual Axis, tilt range: (-65.0°, 63.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection: D. Prabha Dias; processed by Bingni (Wen) Brunton</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ATCC media (made by A.Frangakis);  0.5ml culture in perforated 1.5ml microfuge tube; extended stationary phase; see DPD notebook Jul 23-29, 2004</p>
<strong>Sample Preparation:</strong> Box 119 grid 1, MeOH-washed Quantifoil R2/2, 10nm gold on grids & in suspension, 100% humidity, -3 offset, 1 sec blot, 2 sec drain</p>
  SAVE_Jul30d_x649y366z256_xs.jpg: file associated to 3D Image #217 SAVE_Jul30d_x649y366z256_xy.jpg: file associated to 3D Image #217 SAVE_Jul30d_x649y368z257_xs.jpg: file associated to 3D Image #217 SAVE_Jul30d_x649y368z257_xy.jpg: file associated to 3D Image #217 SAVE_Jul30d_x654y539z274.jpg: file associated to 3D Image #217 SAVE_Jul30d_x655y526z273_xs.jpg: file associated to 3D Image #217 SAVE_Jul30d_x655y526z273_xy.jpg: file associated to 3D Image #217 SAVE_Jul30d_x659y466z267_xs.jpg: file associated to 3D Image #217 SAVE_Jul30d_x659y466z267_xy.jpg: file associated to 3D Image #217 SAVE_Jul30dParM05a.jpg: file associated to 3D Image #217 SAVE_Jul30dParM05b.jpg: file associated to 3D Image #217 SAVE_Jul30drec_01.jpg: file associated to 3D Image #217 SAVE_Jul30drec_02.jpg: file associated to 3D Image #217 SAVE_Jul30drec_03.jpg: file associated to 3D Image #217 SAVE_Jul30drec_04.jpg: file associated to 3D Image #217 SAVE_Jul30drec_05.jpg: file associated to 3D Image #217 SAVE_Jul30drec_mreB.jpg: file associated to 3D Image #217 SAVE_Jul30drec_PM-5px.jpg: file associated to 3D Image #217 SAVE_Jul30drec_xsec02.jpg: file associated to 3D Image #217 SAVE_Jul30drecMreB_himag1.jpg: file associated to 3D Image #217 SAVE_Jul30drecMreB_xsec1.jpg: file associated to 3D Image #217 SAVE_Jul30drecp8.jpg: file associated to 3D Image #217 SAVE_ParMCrossSection3.jpg: file associated to 3D Image #217 SAVE_ParMCrossSection3p.jpg: file associated to 3D Image #217 SAVE_ParMFig.jpg: file associated to 3D Image #217 SAVE_ParMpanel1Jul30d.jpg: file associated to 3D Image #217 pdd2004-07-30-1_slicer5047.jpg: Unknown pdd2004-07-30-1_slicer5049.jpg: Uknown Granule pdd2004-07-30-1_slicer5052.jpg: Unknown Filament pdd2004-07-30-1_slicer5057.jpg: Stacked Membrane pdd2004-07-30-1_slicer5060.jpg: Unknown filament pdd2004-07-30-1_slicer5063.jpg: Unknown filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/rawdata/</p>CC04July30e100t1a.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/rawdata/CC04July30e100t1a.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC04July30e100t1b.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/rawdata/CC04July30e100t1b.mrc"
        > <i class="download icon"></i> Download </a></p>

        2k_sum.rec,  Reconstruction (Pixel Size 2.4 nm), 4.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/3dimage_214/2k_sum.rec"
        > <i class="download icon"></i> Download </a></p>

        CCt1a.rec,  Reconstruction (Pixel Size 2.4 nm), 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/3dimage_215/CCt1a.rec"
        > <i class="download icon"></i> Download </a></p>

        CCt1b.rec,  Reconstruction (Pixel Size 2.4 nm), 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/3dimage_216/CCt1b.rec"
        > <i class="download icon"></i> Download </a></p>

        sum.rec,  Reconstruction (Pixel Size 2.4 nm) Analysis files associated to 3D Image #217 have not been uploaded into the Database. .  Original directory: pd2004-07-30-01/Reconstructions/Full/Analysis/ .  Notes for Full: Keywords: .  extended stationary phase .  crystalline feature .  thick cytoplasmic filament .  100 electrons per tilt series .  dual-axis .  CB15 .  GIFCCD, 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/3dimage_217/sum.rec"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x649y366z256_xs.tif,  file associated to 3D Image #217, 4.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_309/SAVE_Jul30d_x649y366z256_xs.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x649y366z256_xy.tif,  file associated to 3D Image #217, 4.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_311/SAVE_Jul30d_x649y366z256_xy.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x649y368z257_xs.tif,  file associated to 3D Image #217, 4.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_313/SAVE_Jul30d_x649y368z257_xs.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x649y368z257_xy.tif,  file associated to 3D Image #217, 4.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_315/SAVE_Jul30d_x649y368z257_xy.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x654y539z274.tif,  file associated to 3D Image #217, 4.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_317/SAVE_Jul30d_x654y539z274.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x655y526z273_xs.tif,  file associated to 3D Image #217, 4.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_319/SAVE_Jul30d_x655y526z273_xs.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x655y526z273_xy.tif,  file associated to 3D Image #217, 4.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_321/SAVE_Jul30d_x655y526z273_xy.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x659y466z267_xs.tif,  file associated to 3D Image #217, 5.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_323/SAVE_Jul30d_x659y466z267_xs.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30d_x659y466z267_xy.tif,  file associated to 3D Image #217, 4.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_325/SAVE_Jul30d_x659y466z267_xy.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30dParM05a.tif,  file associated to 3D Image #217, 2.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_327/SAVE_Jul30dParM05a.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30dParM05b.tif,  file associated to 3D Image #217, 1.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_329/SAVE_Jul30dParM05b.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_01.tif,  file associated to 3D Image #217, 6.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_331/SAVE_Jul30drec_01.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_02.tif,  file associated to 3D Image #217, 6.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_333/SAVE_Jul30drec_02.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_03.tif,  file associated to 3D Image #217, 6.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_335/SAVE_Jul30drec_03.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_04.tif,  file associated to 3D Image #217, 6.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_337/SAVE_Jul30drec_04.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_05.tif,  file associated to 3D Image #217, 5.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_339/SAVE_Jul30drec_05.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_mreB.tif,  file associated to 3D Image #217, 3.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_341/SAVE_Jul30drec_mreB.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_PM-5px.tif,  file associated to 3D Image #217, 2.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_343/SAVE_Jul30drec_PM-5px.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drec_xsec02.tif,  file associated to 3D Image #217, 5.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_345/SAVE_Jul30drec_xsec02.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drecMreB_himag1.tif,  file associated to 3D Image #217, 5.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_347/SAVE_Jul30drecMreB_himag1.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drecMreB_xsec1.tif,  file associated to 3D Image #217, 5.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_349/SAVE_Jul30drecMreB_xsec1.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_Jul30drecp8.tif,  file associated to 3D Image #217, 2.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_351/SAVE_Jul30drecp8.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_ParMCrossSection3.tiff,  file associated to 3D Image #217, 2.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_353/SAVE_ParMCrossSection3.tiff"
        > <i class="download icon"></i> Download </a></p>

        SAVE_ParMCrossSection3p.tiff,  file associated to 3D Image #217, 3.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_355/SAVE_ParMCrossSection3p.tiff"
        > <i class="download icon"></i> Download </a></p>

        SAVE_ParMFig.tif,  file associated to 3D Image #217, 1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_357/SAVE_ParMFig.tif"
        > <i class="download icon"></i> Download </a></p>

        SAVE_ParMpanel1Jul30d.tif,  file associated to 3D Image #217, 375.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-07-30-1/file_359/SAVE_ParMpanel1Jul30d.tif"
        > <i class="download icon"></i> Download </a></p>