DOI: 10.5061/dryad.k9q7h
title: Data from: Glucocorticoid induces incoordination between glutamatergic and GABAergic neurons in the amygdala

description: Background: Stressful life leads to mood disorders. Chronic mild stress is
 presumably major etiology for depression, and acute severe stress leads to
 anxiety. These stressful situations may impair
 hypothalamus-pituitary-adrenal axis and in turn induce synapse
 dysfunction. However, it remains elusive how the stress hormones mess up
 subcellular compartments and interactions between excitatory and
 inhibitory neurons, which we have investigated in mouse amygdala, a
 structure related to emotional states. Methods and Results: Dexamethasone
 was chronically given by intraperitoneal injection once a day for one week
 or was acutely washed into the brain slices. The neuronal spikes and
 synaptic transmission were recorded by whole-cell patching in amygdala
 neurons of brain slices. The chronic or acute administration of
 dexamethasone downregulates glutamate release as well as upregulates GABA
 release and GABAergic neuron spiking. The chronic administration of
 dexamethasone also enhances the responsiveness of GABA receptors.
 Conclusion: The upregulation of GABAergic neurons and the downregulation
 of glutamatergic neurons by glucocorticoid impair their balance in the
 amygdala, which leads to emotional disorders during stress.
description: 40 mg/kg DEX
 injection-sEPSC Ctrl.12 Inj.19This excel includes all
 original data related to sEPSC recorded on GABAergic neurons from 40 mg/kg
 DEX injection and saline injection mice.injection-sEPSC Ctrl.12
 Inj.19.xlsx40 mg/kg DEX
 injection-sIPSC Ctrl.13 Inj.11This excel includes all
 available original data related to sIPSC recorded on glutamatergic neurons
 in in the mouse amygdala, in which the mice were treated by the
 intraperitoneal injections of dexamethasone (DEX,40 mg/kg) once a day or
 for one week.There are 13 cells in control group and 11 cells in the DEX
 injection group.injection-sIPSC Ctrl.13
 Inj.11.xlsxISI(40 mg/kg DEX
 injection-GABAergic neurons) Ctrl.11 Inj.11This excel includes all
 available original data related to the intervals of spikes(ISI) recorded
 on GABAergic neurons in DEX injection mice(n=11 cells) and in saline
 injection mice(n=11 cells)ISI(injection-IN) Ctrl.11
 Inj.11.xlsxISI(40 mg/kg DEX
 injection-Gluergic neurons) Ctrl.19 Inj.28This excel includes
 original data related to the inter-spike intervals(ISI) recorded on
 Gluergic neurons in DEX injection (n=28 cells) and saline injection(n=19
 cells).ISI(injection-PN) Ctrl.19
 Inj.28.xlsx25 uM DEX washing-on
 sEPSC n=14cellsThis excel includes
 original data related to the inter-spike intervals(ISI) recorded on
 GABAergic neurons(n=14) before and after DEX washing
 on.washing-on EPSC
 14cells.xlsx25 uM DEX washing-on
 IPSC n=10cellsThis excel includes
 original data related to the inter-spike intervals(ISI) recorded on
 Gluergic neurons(n=10) before and after DEX washing on.25 uM DEX washing-on IPSC
 10cells.xlsxISI(25 uM DEX
 washing-on-GABAergic neurons）13CellsThis excel includes
 original data related to the inter-spike intervals(ISI) recorded on
 GABAergic neurons(n=13) before and after DEX washing
 on.ISI(25
 uM DEX washing-on-GABAergic neurons)
 13cells.xlsISI (25 uM DEX
 washing-on-Gluergic neurons) 14cellsThis excel includes
 original data related to the inter-spike intervals recorded on Gluergic
 neurons(n=14) before and after DEX washing on.40 mg/kg DEX
 injectioin-sEPSC Ctrl.12 Inj.19This is an opj. file
 which can be opened by origin software.It includes data related to sIPSC
 on Gluergic neurons from DEX injection and saline injection mice and data
 processing. Moreover,origin software is used to analyse all data related
 to our exprements.40 mg per kg body weight DEX
 injectioin-sEPSC Ctrl.12
 Inj.19.opj40 mg/kg DEX
 injection-sIPSC Ctrl.13 Inj.11This is an opj. file
 which can be opened by origin software.It includes data related to sIPSC
 on Gluergic neurons from DEX injection and saline injection mice and data
 processing.40 mg per kg DEX
 injection-sIPSC Ctrl.13
 Inj.11.opjISI(40 mg/kg DEX
 injection-GABAergic neuron) Ctrl.11 Inj.11An opj.file including
 data related to inter-spike intervals on GABAergic neurons from DEX
 injection and saline injection mice and data
 processing.ISI(DEX injection-GABAergic
 neuron) Ctrl.11 Inj.11.opjISI(40 mg/kg DEX
 injection-Gluergic neuron) Ctrl.19 Inj. 28An opj.file including
 data related to the inter-spike intervals on Gluergic neurons from DEX
 injection and saline injection and data processing.ISI(DEX injection-Gluergic
 neuron ) Ctrl.19 Inj. 28.opj25 uM DEX washing-on
 sEPSC 14cellsAn opj. file including
 data related to sEPSC on GABAergic neurons before and after DEX washing on
 and data processing.25 uM washing-on sEPSC
 14cells.opj25 uM DEX
 washing-on-sIPSC 10cellsAn opj. file including
 data related to sIPSC on Gluergic neurons before and after DEX washing on
 and data processing.25 uM washing-on-IPSC
 10cells.opjISI(25 uM DEX
 washing-on-GABAergic neuron）13CellsAn opj. file including
 data related to inter-spike intervals(ISI) on GABAergic neurons before and
 after DEX washing on and data processing.ISI(25 uM DEX
 washing-on-GABAergic
 neurons）13Cells.opjISI（25 uM DEX
 washing-on-Gluergic neuron）14CellsAn opj. file including
 data related to inter-spike intervals on Gluergic neurons before and after
 DEX washing on and data processing.