DOI: 10.5061/dryad.7qq74
title: Data from: Diurnal and nocturnal transcriptomic variation in the Caribbean staghorn coral, Acropora cervicornis

description: Reef-building corals experience large diel shifts in their environment,
 both externally due to changes in light intensity, predator activity and
 prey availability, and internally as a result of diel fluctuations in
 photosynthesis by their endosymbiotic algae, Symbiodinium. Diel patterns
 of tentacle behaviour, skeletal growth and gene expression indicate
 reactions of the coral animal in response to light and through circadian
 regulation. Some corals, such as the Caribbean Acroporas, have strong
 within-colony division of labour, including specialized fast-growing
 apical polyps, accompanied by large gene expression differences. Here we
 use RNA-seq to evaluate how diel changes in gene expression vary within
 the branching Caribbean staghorn coral, Acropora cervicornis, between
 branch tips and branch bases. Multifactor generalized linear model
 analysis indicated that 6% (3005) of transcripts were differentially
 expressed between branch tips and bases, while 1% (441) of transcripts
 were differentially expressed between day and night. The gene expression
 patterns of 220 transcripts were affected by both time of day and location
 within the colony. In particular, photoreceptors, putative circadian
 genes, stress response genes and metabolic genes were differentially
 expressed between day and night, and some of these, including Amcry1, tef
 and hebp2, exhibited location-specific regulation within the coral colony
 as well. These findings indicate that the genetic response of the coral to
 day and night conditions varies within the colony. Both time of day and
 location within the colony are factors that should be considered in future
 coral gene expression experiments.
description: Normalized gene
 expression data for all samplesThis file contains mRNA
 expression data normalized by library size factor in DEseq for twelve
 Acropora cervicornis samples. The samples were collected from three
 colonies (1-3), two locations on the coral branch (branch base and apical
 tip), at each of two time points (mid-day and
 mid-night).NormData_DNcoral_forDryad.csvColony 1 Day Base Illumina ReadsDemultiplexed Illumina read data for Colony 1, daytime base sample of Acropora cervicornis.C1dayB_trimmed.fastq.zipColony 1 Day Tip Illumina ReadsDemultiplexed Illumina read data for Colony 1, daytime tip sample of Acropora cervicornis.C1dayT_trimmed.fastq.zipColony 1 Night Base Illumina ReadsDemultiplexed Illumina read data for Colony 1, nighttime base sample of Acropora cervicornis.C1nightB_trimmed.fastq.zipColony 1 Night Tip Illumina ReadsDemultiplexed Illumina read data for Colony 1, nighttime tip sample of Acropora cervicornis.C1nightT_trimmed.fastq.zipColony 2 Day Base Illumina ReadsDemultiplexed Illumina read data for Colony 2, daytime base sample of Acropora cervicornis.C2dayB_trimmed.fastq.zipColony 2 Day Tip Illumina ReadsDemultiplexed Illumina read data for Colony 2, daytime tip sample of Acropora cervicornis.C2dayT_trimmed.fastq.zipColony 2 Night Base Illumina ReadsDemultiplexed Illumina read data for Colony 2, nighttime base sample of Acropora cervicornis.C2nightB_trimmed.fastq.zipColony 2 Night Tip Illumina ReadsDemultiplexed Illumina read data for Colony 2, nighttime tip sample of Acropora cervicornis.C2nightT_trimmed.fastq.zipColony 3 Day Base Illumina ReadsDemultiplexed Illumina read data for Colony 3, daytime base sample of Acropora cervicornis.C3dayB_trimmed.fastq.zipColony 3 Day Tip Illumina ReadsDemultiplexed Illumina read data for Colony 3, daytime tip sample of Acropora cervicornis.C3dayT_trimmed.fastq.zipColony 3 Night Base Illumina ReadsDemultiplexed Illumina read data for Colony 3, nighttime base sample of Acropora cervicornis.C3nightB_trimmed.fastq.zipColony 3 Night Tip Illumina ReadsDemultiplexed Illumina read data for Colony 3, nighttime tip sample of Acropora cervicornis.C3nightT_trimmed.fastq.zip