DOI: 10.16904/envidat.221
title: Alnus glutinosa (L.) Gaertn. and Orientus ishidae (Matsumura, 1902) share phytoplasma genotypes linked to the “Flavescence dorée” epidemics

description: Flavescence dorée (FD) is a grapevine disease caused by associated phytoplasmas (FDp), which are epidemically spread by their main vector Scaphoideus titanus. The possible roles of alternative and secondary FDp plant hosts and vectors have gained interest to better understand the FDp ecology and epidemiology. A survey conducted in the surroundings of a vineyard in the Swiss Southern Alps aimed at studying the possible epidemiological role of the FDp secondary vector Orientus ishidae and the FDp host plant Alnus glutinosa is reported. 
Data used for the publication.
Insects were captured by using a sweeping net (on common alder trees) and yellow sticky traps (Rebell Giallo, Andermatt Biocontrol AG, Switzerland) placed in the vineyard canopy. Insects were later determined and selected for molecular analyses.
Grapevines and common alder samples were collected using the standard techniques. The molecular analyses were conducted in order to identify samples infected by the Flavescence dorée phytoplasma (16SrV-p) and the Bois Noir phytoplasma (16SrXII-p). A selection of the infected sampled were further characterized by map genotype and sequenced in order to compare the genotypes in insects, grapevines and common alder trees.
description: Resource Title: Plots description
 Size: 265 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/4724d618-95b5-49a2-bf61-d25ce3d5fd9e/download/table1_4envidat.csv
 Description: Characteristics of the monitored vineyards, including the number of sampled common alders and the number of yellow sticky traps (YST) placed in the canopy.
description: Resource Title: Features of the phytoplasma isolates
 Size: 2613 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/c2103dd5-59f8-4f9c-b931-11e16459cbca/download/table3_4envidat.csv
 Description: Features of the phytoplasma isolates analyzed in this work and reference strains, together with relative GenBank accession numbers and host.
description: Resource Title: Leafhopper captures in vineyards.
 Size: 265 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/f43c4bb2-c011-4372-bcf7-93041657bf5a/download/table4_4envidat.csv
 Description: Leafhoppers captured on yellow sticky traps (YST) placed in vineyards and sampled for molecular analyses. Counts may differ if the quality of the individual specimens was deemed as insufficient for the molecular analysis. Specimens of Scaphoideus titanus caught in Camorino were sampled but not analyzed.
description: Resource Title: Analyzed and infected specimens.
 Size: 801 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/7e68d2e6-2d08-44e8-928b-0e968d624242/download/table5_4envidat.csv
 Description: Number of analyzed and 16SrV-infected specimens collected on common alder trees and on yellow sticky traps (YST) placed inside the vineyard and corresponding infection rate.
description: Resource Title: Results of molecular analyses on a selection of the sampled symptomatic grapevines in Origlio.
 Size: 1047 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/b86bc2f1-6078-462b-80bc-304561386b2a/download/suppl-tables1_4envidat.csv
 Description: Real-time PCR for 16SrV and 16SrXII phytoplasmas, and nested PCR and RFLP digestion (16SrV phytoplasma only). Cq; cycle quantification value; BDL: below detection limit; FDp: “Flavescence dorée” phytoplasma; BNp: “Bois noir” phytoplasma; +: positive response to real-time PCR test; -: negative response to real-time PCR test.
description: Resource Title: Orientus ishidae (development stages L1-2; L3-5; and imago) caught with sweeping net on common alders per plot.
 Size: 384 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/3aba7be1-f8d3-4582-b574-df410bc5c48b/download/suppl-tables2_4envidat.csv
 Description: Number of sampling common alder trees (NAlders) and data regarding captures of O. ishidae (mean, standard deviation (SD), number of observations (NObservations) and total seasonal captures) are reported.
description: Resource Title: Results of the molecular analyses conducted on the sampled common alder trees in Origlio, Camorino, and Montalbano.
 Size: 838 bytes
 Format: application/vnd.ms-excel
 URL: https://www.envidat.ch/dataset/a8aae911-c178-4445-8a6f-c3ad90af1709/resource/f3ac9b03-b4ce-4d18-86b3-f9c829a1d448/download/suppl-tables3_4envidat.csv
 Description: Real-time PCR (conducted on all samples), and nested PCR and RFLP digestion (Origlio only). Cq; cycle quantification value; +: positive response to real-time PCR test; -: negative response to real-time PCR test.