DOI: 10.5281/zenodo.14649225
title: Source data for Chen et al (2024) entitled "Motor Cortical Neuronal Hyperexcitability Associated with α-Synuclein Aggregation"

description:  

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GENERAL INFORMATION 

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This readme file was generated on [2024-01-15] by [Liqiang Chen].

 

Title of Dataset:

Description of Dataset: 

Principal Investigator: Hong-Yuan Chu, hc948@georgetown.edu, ORCID: 0000-0003-0923-683X. 

Date of Data Collection: 2023-04-01 to 2024-11-10 

Software Dependencies: Excel and Image J.

 

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FILE OVERVIEW 

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Directory of Files: Source data, Electrophysiology trace data, and Microscopy images.

Relationship Between Files: Source data is used to make figures in GraphPad. Electrophysiology trace data is used to plot electrophysiology traces. Microscopy images are used for representative images. 

File Formats: Microsoft Excel Worksheet (.xlsx) and confocal images (.nd2)

File Naming Convention: Based on file formats.

 

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DATA SPECIFIC INFORMATION FOR [Source data]

Date of Creation: 2024-08-13

Description of Data: Source data is used to make figures in GraphPad.

A. Missing data are represented n/a.

B. Abbreviations (Primary cortex: M1; Secondary cortex: M2; α-Synulein: αSyn; intratelencephalic neurons: ITNs; corticospinal neurons: CSNs).

C. Figure 5B data (ITN-Sholl analysis-Intersections-5 µm Radius) can not organized as tidy format because of too many data points in each group. 

 

DATA SPECIFIC INFORMATION FOR [Electrophysiology trace data]

Date of Creation: 2024-08-13

Description of Data: Electrophysiology trace data is used to plot electrophysiology traces.

A. Electrophysiology traces can be plotted using Excel.

B. Traces are plotted in Electrophysiology trace data file.

 

DATA SPECIFIC INFORMATION FOR [Microscopy images]

Date of Creation: 2024-08-13

Description of Data: Microscopy images are used to make representative images. 

A. Microscopy images can be opened using Image J. 

B. Microscopy images are named based on experimental group, animal ID, and figure number in the manuscript. 

 

 

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METHODOLOGY

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Description of methods used for data collection: Electrophysiology data is collected using MultiClamp 700B amplifier and Digidata 1550B. pClamp 11 software is used. Microscopy images are collected using an confocal microscope. 

 

Description of methods used for data processing: 

A. Electrophysiology data is processed using clampfit software, including measure the peak of EPSC, count the number of action potentials, measure    the width/rise time/decay time of action potential.

B. Microscopy images are processed using Image J software, including measure the α-Synulein pathologic area in motor cortex, quantify the TH    staining, and verify the co-localization of pS129 and biocytin. 

C. All data after processed through clampfit and Image J is put into GraphPad to make figures.

 

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DATA ACCESS AND SHARING

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This research was funded in part by 

1. Aligning Science Across Parkinson’s (ASAP-020572) through the Michael J. Fox Foundation for Parkinson’s Research (MJFF).

2. National Institute of Neurological Disorders and Stroke (R01NS121374).

3. Congressionally Directed Medical Research Programs (W81XWH-21-1-0943).