cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.21335/nmdc-1473196070-e14
title: CTD profile from 78.68333333333334 N, 14.781116666666666 E in Isfjorden, Svalbard, sampled at 01.11.2022, 08:36 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.68333333333334 N, 14.781116666666666 E at 01.11.2022, 08:36 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e08
title: CTD profile from 78.71001666666666 N, 14.775983333333333 E in Isfjorden, Svalbard, sampled at 01.11.2022, 08:58 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.71001666666666 N, 14.775983333333333 E at 01.11.2022, 08:58 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e07
title: CTD profile from 78.70955 N, 14.730433333333334 E in Isfjorden, Svalbard, sampled at 01.11.2022, 09:10 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.70955 N, 14.730433333333334 E at 01.11.2022, 09:10 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e13
title: CTD profile from 78.70215 N, 14.673716666666667 E in Isfjorden, Svalbard, sampled at 01.11.2022, 09:26 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.70215 N, 14.673716666666667 E at 01.11.2022, 09:26 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e31
title: CTD profile from 78.69273333333334 N, 14.6488 E in Isfjorden, Svalbard, sampled at 01.11.2022, 09:37 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.69273333333334 N, 14.6488 E at 01.11.2022, 09:37 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e32
title: CTD profile from 78.69035 N, 14.588383333333333 E in Isfjorden, Svalbard, sampled at 01.11.2022, 09:45 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.69035 N, 14.588383333333333 E at 01.11.2022, 09:45 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.5281/zenodo.7050538
title: dataset related to the article:"N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin"

description: This record contains raw data related to the article: "N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin" <strong>Abstract: </strong>Human serum albumin (HSA) represents the most abundant plasma protein, with relevant antioxidant activity due to the presence of the sulfhydryl group on cysteine at position 34 (Cys34), the latter being one of the major target sites for redox-dependent modifications leading to the formation of mixed disulfide linkages with low molecular weight thiols. Thiolated forms of HSA (Thio-HSA) may be useful as markers of an unbalanced redox state and as a potential therapeutic target. Indeed, we have previously reported that albumin Cys34 can be regenerated in vitro by <em>N</em>-Acetylcysteine (NAC) through a thiol-disulfide breaking mechanism, with a full recovery of the HSA antioxidant and antiplatelet activities.<strong> </strong>With this pilot study, we aimed to assess the ability of NAC to regenerate native mercaptoalbumin (HSA-SH) and the plasma antioxidant capacity in subjects with redox unbalance, after oral and intravenous administration.<strong> </strong>A placebo-controlled crossover study, single-blinded, was performed on six hypertensive subjects, randomized into two groups, on a one-to-one basis with NAC (600 mg/die, <em>os</em>) or a placebo. Albumin<strong> </strong>isoforms, HSA-SH, Thio-HSA, and glutathione levels were evaluated by means of mass spectrometry. The plasma antioxidant activity was assessed by a fluorimetric assay. NAC, orally administered, significantly decreased the Thio-HSA levels in comparison with the pre-treatment conditions (T0), reaching the maximal effect after 60 min (−24.7 ± 8%). The Thio-HSA reduction was accompanied by a concomitant increase in the native HSA-SH levels (+6.4 ± 2%). After<strong> </strong>intravenous administration of NAC, a significant decrease of the Thio-HSA with respect to the pre-treatment conditions (T0) was observed, with a maximal effect after 30 min (−68.9 ± 10.6% vs. T0) and remaining significant even after 6 h. Conversely, no effect on the albumin isoforms was detected with either the orally or the intravenously administered placebo treatments. Furthermore, the total antioxidant activity of the plasma significantly increased after NAC infusion with respect to the placebo (<em>p </em>=<em> </em>0.0089). Interestingly, we did not observe any difference in terms of total GSH corrected for hemoglobin, ruling out any effect of NAC on the intracellular glutathione and supporting its role as a disulfide-breaking agent.<strong> </strong>This case study confirms the in vitro experiments and demonstrates for the first time that NAC is able to regenerate mercaptoalbumin in vivo,<em> </em>allowing us to hypothesize that<em> </em>the<em> </em>recovery of Cys34 content can modulate in vivo oxidative stress and, hopefully, have an effect in oxidative-based diseases.
DOI: 10.5281/zenodo.7050539
title: dataset related to the article:"N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin"

description: This record contains raw data related to the article: "N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin" <strong>Abstract: </strong>Human serum albumin (HSA) represents the most abundant plasma protein, with relevant antioxidant activity due to the presence of the sulfhydryl group on cysteine at position 34 (Cys34), the latter being one of the major target sites for redox-dependent modifications leading to the formation of mixed disulfide linkages with low molecular weight thiols. Thiolated forms of HSA (Thio-HSA) may be useful as markers of an unbalanced redox state and as a potential therapeutic target. Indeed, we have previously reported that albumin Cys34 can be regenerated in vitro by <em>N</em>-Acetylcysteine (NAC) through a thiol-disulfide breaking mechanism, with a full recovery of the HSA antioxidant and antiplatelet activities.<strong> </strong>With this pilot study, we aimed to assess the ability of NAC to regenerate native mercaptoalbumin (HSA-SH) and the plasma antioxidant capacity in subjects with redox unbalance, after oral and intravenous administration.<strong> </strong>A placebo-controlled crossover study, single-blinded, was performed on six hypertensive subjects, randomized into two groups, on a one-to-one basis with NAC (600 mg/die, <em>os</em>) or a placebo. Albumin<strong> </strong>isoforms, HSA-SH, Thio-HSA, and glutathione levels were evaluated by means of mass spectrometry. The plasma antioxidant activity was assessed by a fluorimetric assay. NAC, orally administered, significantly decreased the Thio-HSA levels in comparison with the pre-treatment conditions (T0), reaching the maximal effect after 60 min (−24.7 ± 8%). The Thio-HSA reduction was accompanied by a concomitant increase in the native HSA-SH levels (+6.4 ± 2%). After<strong> </strong>intravenous administration of NAC, a significant decrease of the Thio-HSA with respect to the pre-treatment conditions (T0) was observed, with a maximal effect after 30 min (−68.9 ± 10.6% vs. T0) and remaining significant even after 6 h. Conversely, no effect on the albumin isoforms was detected with either the orally or the intravenously administered placebo treatments. Furthermore, the total antioxidant activity of the plasma significantly increased after NAC infusion with respect to the placebo (<em>p </em>=<em> </em>0.0089). Interestingly, we did not observe any difference in terms of total GSH corrected for hemoglobin, ruling out any effect of NAC on the intracellular glutathione and supporting its role as a disulfide-breaking agent.<strong> </strong>This case study confirms the in vitro experiments and demonstrates for the first time that NAC is able to regenerate mercaptoalbumin in vivo,<em> </em>allowing us to hypothesize that<em> </em>the<em> </em>recovery of Cys34 content can modulate in vivo oxidative stress and, hopefully, have an effect in oxidative-based diseases.
DOI: 10.21335/nmdc-1473196070-e10
title: CTD profile from 78.67476666666667 N, 14.496016666666666 E in Isfjorden, Svalbard, sampled at 01.11.2022, 12:14 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.67476666666667 N, 14.496016666666666 E at 01.11.2022, 12:14 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e9
title: CTD profile from 78.66928333333334 N, 14.48385 E in Isfjorden, Svalbard, sampled at 01.11.2022, 12:24 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.66928333333334 N, 14.48385 E at 01.11.2022, 12:24 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-e33
title: CTD profile from 78.68118333333334 N, 14.442483333333334 E in Isfjorden, Svalbard, sampled at 01.11.2022, 12:41 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.68118333333334 N, 14.442483333333334 E at 01.11.2022, 12:41 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.5281/zenodo.7248095
title: Dataset related to the article : "The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus"

description: This record contains raw data related to the article: The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus Abstract Human serum albumin (HSA) has an important antioxidant activity due to the presence of the reduced cysteine at position 34, which represents the most abundant free thiol in the plasma. In oxidative-based diseases, HSA undergoes S-thiolation (THIO-HSA) with changes in the antioxidant function of albumin that could contribute to the progression of the disease. The aim of this study was to verify, for the first time, the different burdens of THIO-HSA, glycated HSA (GLY-HSA), and advanced glycation end products (AGE) accumulation both in type 2 diabetes mellitus (T2DM) patients and in non-diabetic patients, with or without coronary heart disease (CHD). In this study, we assessed the presence of modified forms of HSA, THIO-HSA, and GLY-HSA by means of mass spectrometry in 33 patients with both T2DM and CHD, in 31 patients with T2DM and without CHD, in 30 patients without diabetes with a history of CHD, and 27 subjects without diabetes and CHD. All the patients' anthropometric and clinical data were recorded including age, sex, duration of diabetes, body mass index (BMI), blood pressure, and history of CHD defined with anamnestic data. Metabolic parameters, such as fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), lipids, pentosidine, AGE, receptor for advanced glycation end-products (RAGE) and its soluble form (sRAGE), were measured. AGE and pentosidine are significantly higher in T2DM patients with and without CHD with respect to non-diabetic patients with CHD and control subjects. RAGE levels are significantly higher in T2DM patients with respect to non-diabetic patients, and among T2DM patients, the group with CHD showed significantly higher RAGE levels than those without CHD (217 ± 171 pg/mL and 140 ± 61 pg/mL, respectively). Albumin isoforms discriminate between non-diabetic patients with CHD and T2DM patients with and without CHD and control subjects, with GLY-HSA levels higher in T2DM with and without CHD, and THIO-HSA higher in CHD patients without T2DM. Finally, we demonstrated that the oxidized forms of HSA can increase the expression of the inflammatory cytokine Tumor Necrosis Factor-alpha (TNFα) in monocytic cells. In patients with CHD, GLY-HSA and THIO-HSA have a different prevalent distribution, the first one prevailing in patients with T2DM and the second one in patients without T2DM. These findings suggest that albumin quality and homeostasis balance between glyco-oxidation and thiolation might have an impact on the antioxidant defense system in cardiovascular diseases.
description: This work was supported by the Italian Ministry of Health, Rome, Italy (Ricerca Corrente 2021, 2764191, Centro Cardiologico Monzino IRCCS).
DOI: 10.5281/zenodo.7248096
title: Dataset related to the article : "The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus"

description: This record contains raw data related to the article: The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus Abstract Human serum albumin (HSA) has an important antioxidant activity due to the presence of the reduced cysteine at position 34, which represents the most abundant free thiol in the plasma. In oxidative-based diseases, HSA undergoes S-thiolation (THIO-HSA) with changes in the antioxidant function of albumin that could contribute to the progression of the disease. The aim of this study was to verify, for the first time, the different burdens of THIO-HSA, glycated HSA (GLY-HSA), and advanced glycation end products (AGE) accumulation both in type 2 diabetes mellitus (T2DM) patients and in non-diabetic patients, with or without coronary heart disease (CHD). In this study, we assessed the presence of modified forms of HSA, THIO-HSA, and GLY-HSA by means of mass spectrometry in 33 patients with both T2DM and CHD, in 31 patients with T2DM and without CHD, in 30 patients without diabetes with a history of CHD, and 27 subjects without diabetes and CHD. All the patients' anthropometric and clinical data were recorded including age, sex, duration of diabetes, body mass index (BMI), blood pressure, and history of CHD defined with anamnestic data. Metabolic parameters, such as fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), lipids, pentosidine, AGE, receptor for advanced glycation end-products (RAGE) and its soluble form (sRAGE), were measured. AGE and pentosidine are significantly higher in T2DM patients with and without CHD with respect to non-diabetic patients with CHD and control subjects. RAGE levels are significantly higher in T2DM patients with respect to non-diabetic patients, and among T2DM patients, the group with CHD showed significantly higher RAGE levels than those without CHD (217 ± 171 pg/mL and 140 ± 61 pg/mL, respectively). Albumin isoforms discriminate between non-diabetic patients with CHD and T2DM patients with and without CHD and control subjects, with GLY-HSA levels higher in T2DM with and without CHD, and THIO-HSA higher in CHD patients without T2DM. Finally, we demonstrated that the oxidized forms of HSA can increase the expression of the inflammatory cytokine Tumor Necrosis Factor-alpha (TNFα) in monocytic cells. In patients with CHD, GLY-HSA and THIO-HSA have a different prevalent distribution, the first one prevailing in patients with T2DM and the second one in patients without T2DM. These findings suggest that albumin quality and homeostasis balance between glyco-oxidation and thiolation might have an impact on the antioxidant defense system in cardiovascular diseases.
description: This work was supported by the Italian Ministry of Health, Rome, Italy (Ricerca Corrente 2021, 2764191, Centro Cardiologico Monzino IRCCS).
DOI: 10.21335/nmdc-1473196070-11
title: CTD profile from 78.54893333333334 N, 16.284233333333333 E in Isfjorden, Svalbard, sampled at 02.11.2022, 12:55 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.54893333333334 N, 16.284233333333333 E at 02.11.2022, 12:55 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-13
title: CTD profile from 78.54268333333333 N, 16.413916666666665 E in Isfjorden, Svalbard, sampled at 02.11.2022, 13:15 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.54268333333333 N, 16.413916666666665 E at 02.11.2022, 13:15 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-14
title: CTD profile from 78.54008333333333 N, 16.466666666666665 E in Isfjorden, Svalbard, sampled at 02.11.2022, 13:31 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.54008333333333 N, 16.466666666666665 E at 02.11.2022, 13:31 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-15sb
title: CTD profile from 78.50253333333333 N, 16.398033333333334 E in Isfjorden, Svalbard, sampled at 02.11.2022, 13:54 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.50253333333333 N, 16.398033333333334 E at 02.11.2022, 13:54 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-16sb
title: CTD profile from 78.50638333333333 N, 16.3423 E in Isfjorden, Svalbard, sampled at 02.11.2022, 14:13 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.50638333333333 N, 16.3423 E at 02.11.2022, 14:13 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-s1_1
title: CTD profile from 78.42976666666667 N, 17.373666666666665 E in Isfjorden, Svalbard, sampled at 04.11.2022, 07:54 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.42976666666667 N, 17.373666666666665 E at 04.11.2022, 07:54 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.
DOI: 10.21335/nmdc-1473196070-s1_2
title: CTD profile from 78.43018333333333 N, 17.368483333333334 E in Isfjorden, Svalbard, sampled at 04.11.2022, 08:04 UTC during the UNIS AGF311/811-cruise.

description: The file contains temperature, practical salinity and depth measurements binned into 1 db pressure bins. The raw data was measured at 78.43018333333333 N, 17.368483333333334 E at 04.11.2022, 08:04 UTC using a Seabird SBE19v2plus CTD and post-processed with the Seasoft software package. The following processing steps were applied (with default settings): 1. Data Conversion, 2. Wild Edit, 3. Align CTD, 4. Cell Thermal Mass, 5. Filter, 6. Bin Average. If not specified differently, the downcast of the profile was used.