DOI: 10.5061/dryad.3j9kd51hc
title: Data from: The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy (SBEM)

description: The locust’s lobula giant movement detector 1 (LGMD1) looming detector
 pathway is part of the compound eye visual system and enables the animals
 to reliably detect collisions. Trans-medullary afferent neurons are
 considered key players in this pathway. Thousands of these neurons connect
 the second visual neuropile region, or medulla, with the third neuropile
 region, or lobula complex. In the lobula complex they are in synaptic
 contact with the LGMD1, which forms a dendritic tree in the outer region
 of the lobula complex neuropile. In order to describe their anatomy and
 connectivity patterns with other upstream neurons of the LGMD1, we used
 serial block-face scanning electron microscopy. We thus produced serial
 electron micrographs spanning from the dendritic tree in the outer lobula
 complex to the origin of the trans-medullary afferent neurons in the
 medulla. Starting from the LGMD1, we segmented and 3D-reconstructed entire
 trans-medullary afferents, as well as connecting neurons and other
 trans-medullary neurons nearby. This study was based on two datasets from
 different locusts of fourth instar. Here we provide the raw data for this
 study as .tiff stacks.
description: The optic lobe of 2 locusts (Locusta migratoria) of fourth instar
 were fixed, dehydrated, block stained, and embedded in TAAB epoxy resin. A
 block face was trimmed through the outer lobula. Serial scans were made
 from the outer lobula, through the outer chiasm, into the medulla. An
 in-situ ultra-microtome 3View (Gatan, Inc., Pleasanton, CA, USA), mounted
 in the chamber of an environmental scanning electron microscope ESEM
 Quanta 600 FEG (FEI, Eindhoven, Netherlands) at the Graz University of
 Technology was used for image acquisition. Microscope and imaging
 conditions: 3kV acceleration voltage, water vapour with a pressure of 20
 Pa, spot size 3.2, dwell time of 12.5 µs. Backscattered electron detector
 (BSED; Gatan, Inc.). 
description: Wernitznig_2021_Locust_L4_Lobula_To_Medulla_Dataset01 Wernitznig_2021_Locust_L4_Lobula_To_Medulla_Dataset02 These datasets includes the serial sections used in the following paper: Wernitznig, S; Zankel, A; Bock, E; Gütl<sup>, </sup>D; Hobusch, U; Nicolic, M; Pargger, L; Pritz, E; Radulović, S; Sele, M; Summerauer, S; Pölt, P; Rind, FC,  and G. Leitinger. The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy (SBEM) (J. Comp Neurol, doi: 10.1002/cne.25227). Each dataset starts in the outer lobula complex of a locust of fourth instar, and covers the outer lobula, the outer optic chiasm, and the medulla. Voxel size 10 x 10 x 40-50 nm; section. The TmA neuron is kept in the center of the field of view, so the field of view was moved in a lateral direction between the stacks. Image size in dataset one was 25 x 25 µm, for dataset 2, it was 30 x 30 µm in the lobula complex, when nearing the second optic chiasm, and 20 x 40 µm, within the chiasm, 25 x 25 µm, and in the medulla either 30 x 30, or 35 x 35 µm.