DOI: 10.5061/dryad.83bk3jb6q
title: Aphid infestation induces plant-sex-specific changes in floral chemistry and pollinator behaviour in <em>Silene latifolia</em>

description: This Dataset supports the article "Aphid infestation induces
 plant-sex-specific changes in floral chemistry and pollinator behaviour in
 Silene latifolia", which is accepted for publication in the Journal
 of Experimental Botany. The study addresses the combined effects of Silene
 latifolia plant sex and infestation with the oligophagous aphid
 Brachycaudus lychnidis on interactions among Silene latifolia and its
 specialised nursery pollinator Hadena bicruris. It contains data on
 various floral traits (spatial flower traits, flower colour traits, floral
 scent traits (floral VOC), floral rewards), pollinator behaviour in binary
 choice tests and plant stress indicators. The data were assessed in
 control and aphid-infested male and female plants. A detailed description
 of the data acquisition process can be found in the published manuscript.
description: # Aphid infestation induces plant-sex-specific changes in floral chemistry
 and pollinator behaviour in *Silene latifolia* Dataset DOI:
 [10.5061/dryad.83bk3jb6q](https://doi.org/10.5061/dryad.83bk3jb6q) ##
 Description of the data and file structure ### File name: Data.xlsx Below
 you find a list of datasets with a summary of variable names and a brief
 description of the data. Missing values are indicated with “NA”: **Across
 all sheets:** * E_ID: plant individual identity * E_SEX: plant sex
 (F=female, M=male) * E_TMT: aphid infestation treatment (C=control,
 A=infested) **Sheet "PAP_highres": Flower traits** Data in this
 table comprises plant stress-related traits and floral traits measured per
 plant individual. Several traits were strongly correlated and therefore
 summarized into a single response (i.e., a single column) and modelled
 using a categorical co-predictor representing the respective pooled trait
 types. * co-predictor - E_APHIDSIZE: size category of aphids
 (L=large,M=medium,S=small; see publication for explanation) * response -
 NO_APHID: number of aphids of the regarding size per individual *
 co-predictor - E_TFLOBUD: time point of data acquisition (days after aphid
 infestations) for the number of open flowers and flower buds * response -
 NO_FLOBUD: number of open flowers and flower buds per individual *
 co-predictor - E_COMPBIOM: component of dry biomass assessed
 (3shoot=shoot, 2flowers=all flowers and flower buds, 1siflo=single flower)
 * response - BIOM: dry biomass (g) of a given component per individual *
 co-predictor - E_SHAPETYPE: flower component for which area has been
 acquired (1egg=are covered by smallest possible circle drawn around
 flower, 2flo=all petals of a single flower, 3pet=area of one petal) *
 response - SHAPE_AREA: display area (pixel) * additional random factor -
 E_COL_TRIAL: flower colours have been assessed during different time
 points = trials (T1-T3) * co-predictor - E_COLTYPE: wavelength range,
 i.e., quality of reflected light (2G=green, 3B=blue, 4U=UV) * response -
 COL: incoming light reflected by flower (%) * response - NECTAR_VOLUME:
 volume of nectar per flower (µL), note that this was measured repeatedly
 **Sheet "PAP_LSA_joint": Leaf pigments** Data in this table
 includes measurements of leaf light absorbance with an LSA device *
 E_TIME: time point of data acquisition (1before=before aphid infestation,
 2after=after aphid infestation) * AFLAV: absorbance by epidermally located
 flavonoids (proportion) * AANTH: absorbance by epidermally located
 anthocyanins (proportion) * NMOL: chlorophyll content (nmol) **Sheet
 "PAP_choice": Binary choice tests with *Hadena bicruris*** *
 E_TEST: Type of choice test (CHOICETMT=effect of aphid infestation
 treatment within female and male plants, CHOICETMT=effect of plant sex
 within control and infested plants) * ID_MOTH: identity of *Hadena
 bicruris* moth individual * ID_PLANT_I: identity of choice plant 1 *
 ID_PLANT_II: identity of choice plant 2 * SP1: sex of choice plant 1
 (F=female, M=male) * SP2: sex of choice plant 2 (F=female, M=male) * TP1:
 aphid infestation treatment of choice plant 1 (C=control, T=infested) *
 TP2: aphid infestation treatment of choice plant 2 (C=control, T=infested)
 * MATCH: columns ID_MOTH - TP2 merged * E_TRIAL_ID: Trial identity (same
 for all plants with identical entries for MATCH) * SP: sex choice plant 1
 and choice plant 2 (F=female, M=male) * TP: aphid infestation treatment
 choice plant 1 and choice plant 2 (C=control, T=infested) * VISIT: visit
 number (1-4) in total, max four visits per trial were analysed * E_CHOICE:
 behavioural parameter under observation (1vis=visited[1=visited, 0=not
 visited], 2fed=feeding[1=fed,0=not fed], 3time=time of entire visit [s]) *
 CHOICE1: behavioural parameter for a given trial and visit observed for
 plant 1 * CHOICE2: behavioural parameter for a given visit and trial
 observed for plant 2 **Sheet "Scent_metabolome": Relative
 intensities (intensity of individual compound/intensity of internal
 standard) of floral volatile organic compounds (VOC) in *Silene
 latifolia*** * C01-C42 relative intensities of compound IDs C01-C42 (IDs
 correspond to metabolite identities in Supplementary Table S1A) **Sheet
 "Volcano_scent": Dataset for generation of volcano plots for
 floral scent composition in** ***Silene latifolia*** * metabolite ID:
 compound ID (IDs correspond to metabolite identities in Supplementary
 Table S1A) * log2FC: log2(relative compound intensity infested/relative
 compound intensity control) * pval=p-value uncorrected * pvalcorr=p-value
 FDR corrected **Sheet "Nectar_metabolome": Intensities of
 metabolites in the nectar of** ***Silene latifolia*** * ID0001- ID4774
 intensities of compound IDs ID0001- ID4774 (IDs correspond to metabolite
 identities in Supplementary Table S1B) **Sheet "Volcano_nectar
 ": Dataset for generation of volcano plots for floral nectar
 composition in** ***Silene latifolia*** * ID: compound ID (IDs correspond
 to metabolite identities in Supplementary Table S1A) * mean_F: mean
 intensity in female plants * mean_M: mean intensity in male plants *
 mean_C: mean intensity in control plants * mean_A: mean intensity in
 aphid-infested plants * mean_FC: mean intensity in female control plants *
 mean_FA: mean intensity in female-infested plants * mean_MC: mean
 intensity in male control plants * mean_MA: mean intensity in
 male-infested plants * log2_F-M: log2(mean intensity female/mean intensity
 male) * log2_A-C: log2(mean intensity infested/mean intensity control) *
 log2_FA-FC: log2(mean intensity infested female/mean intensity control
 female) * log2_MA-MC: log2(mean intensity infested male/mean intensity
 control male) * log2_FC-MC: log2(mean intensity control female/mean
 intensity control male) * log2_FA-MA: log2(mean intensity infested
 female/mean intensity infested male) * OUTCOME CHECKING: outcome of best
 model loop (UNT=untransformed response, SQRT=square root transformed
 response, LOG=log10 transformed response) * pun_F-M: uncorrected p-value
 sex effect * pcorr_F-M: FDR corrected p-value sex effect * mod_F-M:
 modulation sex effect (un=not modulated [log2FC&lt;1/&gt;-1 &
 uncorrected p-value &gt;0.05], mod =modulated [log2FC&gt;1/&lt;-1 &
 uncorrected p-value &lt;0.05], modfd=modulated [log2FC&gt;1/&lt;-1 &
 FDR corrected p-value &lt;0.05]) * pun_A-C: uncorrected p-value aphid
 infestation effect * pcorr_A-C: FDR corrected p-value aphid infestation
 effect * mod_A-C: modulation aphid infestation effect (un=not modulated
 [log2FC&lt;1/&gt;-1 & uncorrected p-value &gt;0.05], mod =modulated
 [log2FC&gt;1/&lt;-1 & uncorrected p-value &lt;0.05], modfd=modulated
 [log2FC&gt;1/&lt;-1 & FDR corrected p-value &lt;0.05]) * pun_FA-FC:
 uncorrected p-value aphid infestation effect within female plants *
 pcorr_FA-FC: FDR corrected p-value of aphid infestation effect within
 female plants * mod_FA-FC: modulation aphid infestation effect within
 female plants (un=not modulated [log2FC&lt;1/&gt;-1 & uncorrected
 p-value &gt;0.05], mod =modulated [log2FC&gt;1/&lt;-1 & uncorrected
 p-value &lt;0.05], modfd=modulated [log2FC&gt;1/&lt;-1 & FDR corrected
 p-value &lt;0.05]) * pun_MA-MC: uncorrected p-value of aphid infestation
 effect within male plants * pcorr_MA-MC: FDR corrected p-value of aphid
 infestation effect within male plants * mod_MA-MC: modulation aphid
 infestation effect within male plants (un=not modulated
 [log2FC&lt;1/&gt;-1 & uncorrected p-value &gt;0.05], mod =modulated
 [log2FC&gt;1/&lt;-1 & uncorrected p-value &lt;0.05], modfd=modulated
 [log2FC&gt;1/&lt;-1 & FDR corrected p-value &lt;0.05]) * pun_FC-MC:
 uncorrected p-value sex effect within control plants * pcorr_FC-MC: FDR
 corrected p-value sex effect within control plants * mod_FC-MC: modulation
 sex effect within control plants (un=not modulated [log2FC&lt;1/&gt;-1
 & uncorrected p-value &gt;0.05], mod =modulated [log2FC&gt;1/&lt;-1
 & uncorrected p-value &lt;0.05], modfd=modulated [log2FC&gt;1/&lt;-1
 & FDR corrected p-value &lt;0.05]) * pun_FA-MA: uncorrected p-value
 sex effect within infested plants * pcorr_FA-MA: FDR corrected p-value sex
 effect within infested plants * mod_FA-MA: : modulation sex effect within
 infested plants (un=not modulated [log2FC&lt;1/&gt;-1 & uncorrected
 p-value &gt;0.05], mod =modulated [log2FC&gt;1/&lt;-1 & uncorrected
 p-value &lt;0.05], modfd=modulated [log2FC&gt;1/&lt;-1 & FDR corrected
 p-value &lt;0.05])   ## Code/software None ## Access information Other
 publicly accessible locations of the data: * Not applicable Data was
 derived from the following sources: * Experiment published in
 10.1093/jxb/eraf522