cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.34808/3k5y-3716
title: Other diseases of jaws - Female, 9 - Tissue image [6110730021448661]

description: This is the histopathological image of BONES, JOINTS AND ARTICULAR CARTILAGE OF OTHER AND UNSPECIFIED SITES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 9Clinical description: Gender: FemaleDiagnosis:Classification: ICD-10_10-20Classification code: Chapter XIII - Diseases of the musculoskeletal system and connective tissueDiagnosis: Other diseases of jawsResult of the histopathological examination: Sample:Material: FFPECollecting method: Surgical specimenTopography: BONES, JOINTS and ARTICULAR CARTILAGEOrgan: BONES, JOINTS AND ARTICULAR CARTILAGE OF OTHER AND UNSPECIFIED SITESTissue: MandibleType of staining: positive/IHCStaining: Not applicableAntibody: Ki-67Technology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/e5wj-7w66
title: Diffuse large B-cell lymphoma, activated B-cell subtype - Male, 63 - Tissue image [6120730031822651]

description: This is the histopathological image of UNKNOWN PRIMARY SITE tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 63Clinical description: Suspicion of DLBCL.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: MATURE B-CELL LYMPHOMASDiagnosis: Diffuse large B-cell lymphoma, activated B-cell subtypeResult of the histopathological examination: DLBCL non-GCB type: CD20+, bcl2+, bcl6+, cmyc focally+ (Sample:Material: FFPECollecting method: Surgical biopsyTopography: UNKNOWN PRIMARY SITEOrgan: UNKNOWN PRIMARY SITETissue: Unknown primary siteType of staining: positive/IHCStaining: Not applicableAntibody: BCL2Technology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/69k4-j271
title: Neuroendocrine tumor, grade 1 - Female, 78 - Tissue image [6120730031815501]

description: This is the histopathological image of PANCREAS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 78Clinical description: Tumor of tail of pancreas. Suspicion of NET.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: ADENOMAS AND ADENOCARCINOMASDiagnosis: Neuroendocrine tumor, grade 1Result of the histopathological examination: Neuroendocrine tumor of pancreas (NET G1): CKAE1/AE3+, SYN+, CHR+, Ki-67 1%.Sample:Material: FFPECollecting method: Core-needle biopsyTopography: DIGESTIVE ORGANSOrgan: PANCREASTissue: Tail of pancreasType of staining: positive/IHCStaining: Not applicableAntibody: Ki-67Technology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/5tdy-y964
title: Merkel cell carcinoma - Female, 74 - Tissue image [6130730026864231]

description: This is the histopathological image of SKIN tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 74Clinical description: Tumor of skin of thigh.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: ADENOMAS AND ADENOCARCINOMASDiagnosis: Merkel cell carcinomaResult of the histopathological examination: Merkel cell carcinoma: CK20+, CD56+, NF+, SYN+, TTF1-, Ki-67 70%.Sample:Material: FFPECollecting method: Excision of lesionsTopography: SKINOrgan: SKINTissue: Skin of thighType of staining: positive/IHCStaining: Not applicableAntibody: NeurofilamentTechnology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/cjb1-qw23
title: Granulosa cell tumor, adult type - Female, 65 - Tissue image [614073001611791]

description: This is the histopathological image of OVARY tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 65Clinical description: Tumor of the ovary. Tumor of the colon.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: SPECIALIZED GONADAL NEOPLASMSDiagnosis: Granulosa cell tumor, adult typeResult of the histopathological examination: Adult granulosa cell tumor. IHC: WT1(+), Inhibin(+), Calretinin (+), CK7(-), CK20(-), CDX2(-), Ki67 2%.Sample:Material: FFPECollecting method: Surgical specimenTopography: FEMALE GENITAL ORGANSOrgan: OVARYTissue: OvaryType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/a0zd-hd92
title: Plasmablastic lymphoma - Female, 62 - Tissue image [6110730021434441]

description: This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: VS200 Olympus slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 62Clinical description: Lymphadenopathy.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: PLASMA CELL TUMORSDiagnosis: Plasmablastic lymphomaResult of the histopathological examination: WHO: plasmablastic lymphoma.

IHC: CD138 (+), MUM1 (+), CD20(-), CD3(-), PAX5(-), bcl2(-), CDCD10(-), bcl6(-), CD79a(-), c-myc(+), CD30: focal(+), MPO(-), Tdt(-), CD117(-), Ki67: 80-90%.Sample:Material: FFPECollecting method: Surgical specimenTopography: LYMPH NODESOrgan: LYMPH NODESTissue: Supraclavicular lymph nodeType of staining: positive/IHCStaining: Not applicableAntibody: MyeloperoxidaseTechnology:Equipment: VS200 OlympusLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/dnhy-h141
title: Squamous cell carcinoma, HPV positive - Female, 43 - Tissue image [6130730026851221]

description: This is the histopathological image of VULVA tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 43Clinical description: Tumor of right labium majus of vulva with dimensions 2,1 x 1,6 x 0,8 cm.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: SQUAMOUS CELL NEOPLASMSDiagnosis: Squamous cell carcinoma, HPV positiveResult of the histopathological examination: Squamous cell carcinoma of vulva (HPV associated) p16+, CKHMW+, Ki-67 80%. VIN 3.Sample:Material: FFPECollecting method: Excision of lesionsTopography: FEMALE GENITAL ORGANSOrgan: VULVATissue: Labium majusType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/chnp-9f17
title: Granulosa cell tumor, adult type - Female, 65 - Tissue image [6140730016116031]

description: This is the histopathological image of OVARY tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 65Clinical description: Tumor of the ovary. Tumor of the colon.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: SPECIALIZED GONADAL NEOPLASMSDiagnosis: Granulosa cell tumor, adult typeResult of the histopathological examination: Adult granulosa cell tumor. IHC: WT1(+), Inhibin(+), Calretinin (+), CK7(-), CK20(-), CDX2(-), Ki67 2%.Sample:Material: FFPECollecting method: Surgical specimenTopography: FEMALE GENITAL ORGANSOrgan: OVARYTissue: OvaryType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.6084/m9.figshare.19085883
title: Development of a standard operating procedure for the DCFH<sub>2</sub>-DA acellular assessment of reactive oxygen species produced by nanomaterials

description: Improved strategies are required for testing nanomaterials (NMs) to make hazard and risk assessment more efficient and sustainable. Including reduced reliance on animal models, without decreasing the level of human health protection. Acellular detection of reactive oxygen species (ROS) may be useful as a screening assay to prioritize NMs of high concern. To improve reliability and reproducibility, and minimize uncertainty, a standard operating procedure (SOP) has been developed for the detection of ROS using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH<sub>2</sub>-DA) assay. The SOP has undergone an inter- and intra-laboratory comparison, to evaluate robustness, reliability, and reproducibility, using representative materials (ZnO, CuO, Mn<sub>2</sub>O<sub>3</sub>, and BaSO<sub>4</sub> NMs), and a number of calibration tools to normalize data. The SOP includes an NM positive control (nanoparticle carbon black (NPCB)), a chemical positive control (SIN-1), and a standard curve of fluorescein fluorescence. The interlaboratory comparison demonstrated that arbitrary fluorescence units show high levels of partner variability; however, data normalization improved variability. With statistical analysis, it was shown that the SIN-1 positive control provided an extremely high level of reliability and reproducibility as a positive control and as a normalization tool. The NPCB positive control can be used with a relatively high level of reproducibility, and in terms of the representative materials, the reproducibility CuO induced-effects was better than for Mn<sub>2</sub>O<sub>3</sub>. Using this DCFH<sub>2</sub>-DA acellular assay SOP resulted in a robust intra-laboratory reproduction of ROS measurements from all NMs tested, while effective reproduction across different laboratories was also demonstrated; the effectiveness of attaining reproducibility within the interlaboratory assessment was particle-type-specific.
DOI: 10.6084/m9.figshare.19085883.v1
title: Development of a standard operating procedure for the DCFH<sub>2</sub>-DA acellular assessment of reactive oxygen species produced by nanomaterials

description: Improved strategies are required for testing nanomaterials (NMs) to make hazard and risk assessment more efficient and sustainable. Including reduced reliance on animal models, without decreasing the level of human health protection. Acellular detection of reactive oxygen species (ROS) may be useful as a screening assay to prioritize NMs of high concern. To improve reliability and reproducibility, and minimize uncertainty, a standard operating procedure (SOP) has been developed for the detection of ROS using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH<sub>2</sub>-DA) assay. The SOP has undergone an inter- and intra-laboratory comparison, to evaluate robustness, reliability, and reproducibility, using representative materials (ZnO, CuO, Mn<sub>2</sub>O<sub>3</sub>, and BaSO<sub>4</sub> NMs), and a number of calibration tools to normalize data. The SOP includes an NM positive control (nanoparticle carbon black (NPCB)), a chemical positive control (SIN-1), and a standard curve of fluorescein fluorescence. The interlaboratory comparison demonstrated that arbitrary fluorescence units show high levels of partner variability; however, data normalization improved variability. With statistical analysis, it was shown that the SIN-1 positive control provided an extremely high level of reliability and reproducibility as a positive control and as a normalization tool. The NPCB positive control can be used with a relatively high level of reproducibility, and in terms of the representative materials, the reproducibility CuO induced-effects was better than for Mn<sub>2</sub>O<sub>3</sub>. Using this DCFH<sub>2</sub>-DA acellular assay SOP resulted in a robust intra-laboratory reproduction of ROS measurements from all NMs tested, while effective reproduction across different laboratories was also demonstrated; the effectiveness of attaining reproducibility within the interlaboratory assessment was particle-type-specific.
DOI: 10.6084/m9.figshare.19344788
title: Long non-coding RNA maternally expressed 3 (MEG3) regulates isoflurane-induced cognitive dysfunction by targeting miR-7-5p

description: This study aimed to investigate the role and mechanism of long non-coding RNA maternally expressed gene 3 (MEG3) in cognitive dysfunction induced by isoflurane (ISO). Morrier water maze analysis was performed to evaluate the cognitive function of rats. Modified modified neurological severity score (mNSS) scores were assessed for neurological damage. The levels of MEG3 in hippocampal tissues of rats and hippocampal neuron cell lines HT22 were examined by reverse transcription-quantitative polymerase chain reaction (qRT-PCR). Moreover, the cell viability and apoptosis were assessed by the Cell Counting Kit-8 (CCK-8) and flow cytometry assay. Indicators of inflammation and oxidative stress were determined using enzyme-linked immunosorbent assay (ELISA) and commercial assay kits. Relationship between MEG3 and microRNA (miR)-7-5p was verified by the dual-luciferase reporter gene assay. MEG3 was increased in hippocampal tissues and HT22 after ISO treatment (<i>p</i> &lt; 0.05). MEG3 downregulation alleviated the increase in neurological severity score and cognitive dysfunction caused by ISO treatment (<i>p</i> &lt; 0.05). In vitro, MEG3 downregulation alleviates the decrease in cell activity and increased apoptosis induced by ISO. What’s more, MEG3 reduction eliminated activation of neuroinflammation and oxidative stress promoted by ISO treatment in rats and HT22 (<i>p</i> &lt; 0.05). MEG3 was confirmed to specifically bind to miR-7-5p. Inhibition of miR-7-5p eliminated the alleviating effects of MEG3 downregulation on cognitive dysfunction caused by ISO treatment. Decreased MEG3 alleviates cognitive dysfunction caused by ISO by targeting miR-7-5p and play a neuroprotective effect. We present a strategy for MEG3 as a potential target for brain protection during anesthesia.
DOI: 10.6084/m9.figshare.19344788.v1
title: Long non-coding RNA maternally expressed 3 (MEG3) regulates isoflurane-induced cognitive dysfunction by targeting miR-7-5p

description: This study aimed to investigate the role and mechanism of long non-coding RNA maternally expressed gene 3 (MEG3) in cognitive dysfunction induced by isoflurane (ISO). Morrier water maze analysis was performed to evaluate the cognitive function of rats. Modified modified neurological severity score (mNSS) scores were assessed for neurological damage. The levels of MEG3 in hippocampal tissues of rats and hippocampal neuron cell lines HT22 were examined by reverse transcription-quantitative polymerase chain reaction (qRT-PCR). Moreover, the cell viability and apoptosis were assessed by the Cell Counting Kit-8 (CCK-8) and flow cytometry assay. Indicators of inflammation and oxidative stress were determined using enzyme-linked immunosorbent assay (ELISA) and commercial assay kits. Relationship between MEG3 and microRNA (miR)-7-5p was verified by the dual-luciferase reporter gene assay. MEG3 was increased in hippocampal tissues and HT22 after ISO treatment (<i>p</i> &lt; 0.05). MEG3 downregulation alleviated the increase in neurological severity score and cognitive dysfunction caused by ISO treatment (<i>p</i> &lt; 0.05). In vitro, MEG3 downregulation alleviates the decrease in cell activity and increased apoptosis induced by ISO. What’s more, MEG3 reduction eliminated activation of neuroinflammation and oxidative stress promoted by ISO treatment in rats and HT22 (<i>p</i> &lt; 0.05). MEG3 was confirmed to specifically bind to miR-7-5p. Inhibition of miR-7-5p eliminated the alleviating effects of MEG3 downregulation on cognitive dysfunction caused by ISO treatment. Decreased MEG3 alleviates cognitive dysfunction caused by ISO by targeting miR-7-5p and play a neuroprotective effect. We present a strategy for MEG3 as a potential target for brain protection during anesthesia.
DOI: 10.34808/1mr3-8g29
title: Diffuse large B-cell lymphoma, NOS - Male, 49 - Tissue image [6120730031827911]

description: This is the histopathological image of TESTIS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 49Clinical description: Tumor of left testis.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: MATURE B-CELL LYMPHOMASDiagnosis: Diffuse large B-cell lymphoma, NOSResult of the histopathological examination: DLBCL: CD20+, Pax5+, bcl2+, bcl6+, MUM1 focally+, CD10 focally+, c-myc + in 5% of nuclei, CD3-, CyclinD1-, Ki-67 90%.Sample:Material: FFPECollecting method: Surgical specimenTopography: MALE GENITAL ORGANSOrgan: TESTISTissue: Testis, NOSType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/dh0e-z737
title: Squamous cell carcinoma, HPV positive - Male, 69 - Tissue image [6130730026863501]

description: This is the histopathological image of BASE OF TONGUE tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 69Clinical description: Tumor of base of tonsil.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: SQUAMOUS CELL NEOPLASMSDiagnosis: Squamous cell carcinoma, HPV positiveResult of the histopathological examination: Squamous cell carcinoma of tongue (HPV associated) p16+.Sample:Material: FFPECollecting method: Excision of lesionsTopography: LIP, ORAL CAVITY AND PHARYNXOrgan: BASE OF TONGUETissue: Base of tongue, NOSType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/fa28-ap25
title: Follicular lymphoma, NOS - Male, 60 - Tissue image [6140730016117081]

description: This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 60Clinical description: Lymphadenopathy.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: MATURE B-CELL LYMPHOMASDiagnosis: Follicular lymphoma, NOSResult of the histopathological examination: Follicular lymphoma.Sample:Material: FFPECollecting method: Surgical specimenTopography: LYMPH NODESOrgan: LYMPH NODESTissue: Subclavicular lymph nodeType of staining: positive/IHCStaining: Not applicableAntibody: CD 23Technology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/ac4b-s244
title: Hodgkin lymphoma, nodular sclerosis, NOS - Female, 13 - Tissue image [6110730021449651]

description: This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 13Clinical description: Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: CHOROBA HODGKINADiagnosis: Hodgkin lymphoma, nodular sclerosis, NOSResult of the histopathological examination: Sample:Material: FFPECollecting method: Surgical specimenTopography: LYMPH NODESOrgan: LYMPH NODESTissue: Supraclavicular lymph nodeType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/4k82-ty18
title: Plasmablastic lymphoma - Female, 62 - Tissue image [6110730021437251]

description: This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: VS200 Olympus slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 62Clinical description: Lymphadenopathy.Gender: FemaleDiagnosis:Classification: ICD-O_3.2Classification code: PLASMA CELL TUMORSDiagnosis: Plasmablastic lymphomaResult of the histopathological examination: WHO: plasmablastic lymphoma.

IHC: CD138 (+), MUM1 (+), CD20(-), CD3(-), PAX5(-), bcl2(-), CDCD10(-), bcl6(-), CD79a(-), c-myc(+), CD30: focal(+), MPO(-), Tdt(-), CD117(-), Ki67: 80-90%.Sample:Material: FFPECollecting method: Surgical specimenTopography: LYMPH NODESOrgan: LYMPH NODESTissue: Supraclavicular lymph nodeType of staining: positive/IHCStaining: Not applicableAntibody: CD 138Technology:Equipment: VS200 OlympusLens: 20xOrganization:Source: Medical University Gdańsk
DOI: 10.34808/zsxg-ve54
title: Spindle cell lipoma - Male, 65 - Tissue image [6130730026868461]

description: This is the histopathological image of CONNECTIVE, SUBCUTANEOUS AND OTHER SOFT TISSUES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 65Clinical description: Tumor of subcutaneous tissue.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: LIPOMATOUS NEOPLASMSDiagnosis: Spindle cell lipomaResult of the histopathological examination: Spindle cell lipoma: CD34+, S100+, MDM2-, Ki-67 Sample:Material: FFPECollecting method: Excision of lesionsTopography: CONNECTIVE, SUBCUTANEOUS AND OTHER SOFT TISSUESOrgan: CONNECTIVE, SUBCUTANEOUS AND OTHER SOFT TISSUESTissue: Connective, subcutaneous and other soft tissues, NOSType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 1000Lens: 20xOrganization:Source: Medical University Gdańsk