cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/jwtfp-ec521
title: bio12-2_lam5_atlas

description: .  [added 2019-10-14 12:31:18] High reZ Atlas of the lamella done at 19500x mag, with preview settings to keep the dose low (0.096 exposure time)
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 0.0 μm,  magnification: 0x. </p>
<strong>Microscope:</strong> 0</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> </p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-30/rawdata/</p>atlas_lam5.mrc,  Tilt Series (Pixel Size 0.0 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-30/rawdata/atlas_lam5.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nepxg-4mn61
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-108/rawdata/</p>Square3Map_Grid3.mrc,  Tilt Series (Pixel Size 0.39 nm), 576.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-108/rawdata/Square3Map_Grid3.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/532yw-ppf79
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-82/rawdata/</p>dH_LG_64step2_53.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-82/rawdata/dH_LG_64step2_53.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fmprh-d4621
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-95/rawdata/</p>Pseudomonas_JT2944Tilt520.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-95/rawdata/Pseudomonas_JT2944Tilt520.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ed4t6-acq95
title: M. xanthus pilA - pilY1.3-flag

description: Raw data files of M. xanthus pilA - pilY1.3-flag
description: <strong>Tilt Series Date:</strong> 2020-01-31</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Yi-Wei Chang, Anke Treuner-Lange, Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated 15 mL 1% CTT with 1mL previously frozen glycerol stock. . Incubated at 32C/225rpm for 2 days.</p>
<strong>Sample Preparation:</strong> Harvested, mixed 40uL cells with 10nm Au fiducials, plunge froze 4uL . (bf 6, bt 6s, d 1s)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-16/rawdata/</p>pilY13_2_022_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-16/rawdata/pilY13_2_022_af.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bcrke-78e21
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-85/rawdata/</p>dH_LG_64step2_56.mrc,  Tilt Series (Pixel Size 2.678 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-85/rawdata/dH_LG_64step2_56.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qv0fe-p0h02
title: B. diazoefficiens, hopanoids

description: WT strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-50/rawdata/</p>WT_LG_64step2_5.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-50/rawdata/WT_LG_64step2_5.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a2njj9
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Paspalum plicatulum",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013681-250827131500795/datasets/export for details.
DOI: 10.22002/4251q-ftd07
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-17</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-61/rawdata/</p>targ59.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-61/rawdata/targ59.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n3ndk-kbg43
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-81/rawdata/</p>dH_LG_64step2_52.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-81/rawdata/dH_LG_64step2_52.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.t3kepx
title: Occurrence Download

description: A dataset containing 137 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Paspalum plicatulum",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 137 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013680-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bsmra-hnb07
title: bio12-2_lam4_atlas

description: .  [added 2019-10-14 12:31:18] High reZ Atlas of the lamella done at 19500x mag, with preview settings to keep the dose low (0.096 exposure time)
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 0.0 μm,  magnification: 0x. </p>
<strong>Microscope:</strong> 0</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> </p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-29/rawdata/</p>atlas_lam4.mrc,  Tilt Series (Pixel Size 0.0 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-29/rawdata/atlas_lam4.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t79e6-jx897
title: M. xanthus pilA - pilY1.3-flag

description: Raw data files of M. xanthus pilA - pilY1.3-flag
description: <strong>Tilt Series Date:</strong> 2020-01-31</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Yi-Wei Chang, Anke Treuner-Lange, Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Inoculated 15 mL 1% CTT with 1mL previously frozen glycerol stock. . Incubated at 32C/225rpm for 2 days.</p>
<strong>Sample Preparation:</strong> Harvested, mixed 40uL cells with 10nm Au fiducials, plunge froze 4uL . (bf 6, bt 6s, d 1s)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-12/rawdata/</p>pilY13_2_017_af.mrc,  Tilt Series (Pixel Size 0.336 nm), 5.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-01-31-12/rawdata/pilY13_2_017_af.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.bd988t
title: Occurrence Download

description: A dataset containing 25 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Paspalum plicatulum",
 "Country is El Salvador",
 "HasCoordinate is true"
 ]
}

 The dataset includes 25 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0013682-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/14c65-bgv62
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-86/rawdata/</p>JV9298_PD-1-3_451.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.6 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f3s2k-c0j20
title: bio12-2_lam3_ts1

description: .  [added 2019-10-11 16:53:00] IT 0.8s
description: <strong>Tilt Series Date:</strong> 2019-09-08</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Gluconacetobacter hansenii</p>
<strong>Strain:</strong> ATCC 23769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: °, constant angular increment, dosage: 88.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions</p>
<strong>Sample Preparation:</strong> Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-09-08-8/rawdata/</p>bio12-2_lam3_ts1.st,  Tilt Series (Pixel Size 0.48 nm), 1.6 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cn0mq-2ea24
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-85/rawdata/</p>Pseudomonas_JT2944Tilt508.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
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        > <i class="download icon"></i> Download </a></p>