cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14285122.v1
title: Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

description: INTRODUCTION: The present study aimed to survey the Vibrio microbiota of oysters (Crassostrea rhizophorae) obtained from restaurants in Fortaleza, State of Ceará, Brazil, and to identify virulence factors. METHODS: The isolated vibrios were submitted to biochemical identification and were tested for hemolytic and urease activities. RESULTS: The isolated strains belonged to 13 species, with predominance of Vibrio mimicus. Of the strain isolates only from fresh samples, 20.5% and 2.8% showed hemolytic and urease activities, respectively. CONCLUSIONS: The findings support the little-publicized claim that Vibrio species other than V. parahaemolyticus and V. vulnificus can represent a health risk to public health.
DOI: 10.6084/m9.figshare.14277460
title: Challenges in diagnosis of genital ulcers: a genital leishmaniasis case series

description: Abstract Leishmaniasis is a tropical infectious disease caused by Leishmania spp. protozoa and is transmitted by insects from the Phlebotominae subfamily. It can manifest as cutaneous leishmaniasis, a painless ulcer that can develop into a more serious systemic affliction as the protozoa spreads lymphatically or hematogenously, depending on the host's immunity. In this case series, the authors present a rare form of genital mucocutaneous leishmaniasis, with consideration of epidemiologic characteristics, clinical presentation, differential diagnosis, and treatments offered.
DOI: 10.6084/m9.figshare.14277460.v1
title: Challenges in diagnosis of genital ulcers: a genital leishmaniasis case series

description: Abstract Leishmaniasis is a tropical infectious disease caused by Leishmania spp. protozoa and is transmitted by insects from the Phlebotominae subfamily. It can manifest as cutaneous leishmaniasis, a painless ulcer that can develop into a more serious systemic affliction as the protozoa spreads lymphatically or hematogenously, depending on the host's immunity. In this case series, the authors present a rare form of genital mucocutaneous leishmaniasis, with consideration of epidemiologic characteristics, clinical presentation, differential diagnosis, and treatments offered.
DOI: 10.6084/m9.figshare.14277415
title: Risk factors associated with mortality in patients hospitalized for coronavirus disease 2019 in Rio de Janeiro, Brazil

description: Abstract INTRODUCTION: Understanding the mortality-associated risk factors of coronavirus disease 2019 will impact clinical decisions. METHODS: This retrospective longitudinal study included patients hospitalized for coronavirus disease in Rio de Janeiro, Brazil. The Kaplan-Meier method and multivariate Cox regression analysis were used. RESULTS: Sequential Organ Failure Assessment score of ≥2 (hazard ratio 4.614; 95% confidence interval =2.210-9.634; p&lt;0.001) and neutrophil/lymphocyte ratio of &gt;5 (hazard ratio=2.616; 95% confidence interval=1.303-5.252; p=0.007) were independently associated with mortality. CONCLUSIONS: Sequential Organ Failure Assessment score and neutrophil/lymphocyte ratio on admission can identify coronavirus disease patients at increased risk of death and guide subsequent clinical decisions.
DOI: 10.6084/m9.figshare.14277415.v1
title: Risk factors associated with mortality in patients hospitalized for coronavirus disease 2019 in Rio de Janeiro, Brazil

description: Abstract INTRODUCTION: Understanding the mortality-associated risk factors of coronavirus disease 2019 will impact clinical decisions. METHODS: This retrospective longitudinal study included patients hospitalized for coronavirus disease in Rio de Janeiro, Brazil. The Kaplan-Meier method and multivariate Cox regression analysis were used. RESULTS: Sequential Organ Failure Assessment score of ≥2 (hazard ratio 4.614; 95% confidence interval =2.210-9.634; p&lt;0.001) and neutrophil/lymphocyte ratio of &gt;5 (hazard ratio=2.616; 95% confidence interval=1.303-5.252; p=0.007) were independently associated with mortality. CONCLUSIONS: Sequential Organ Failure Assessment score and neutrophil/lymphocyte ratio on admission can identify coronavirus disease patients at increased risk of death and guide subsequent clinical decisions.
DOI: 10.6084/m9.figshare.14277417
title: COVID-19 in children in the state of Pernambuco: Spatial analysis of confirmed severe cases and the Human Development Index

description: Abstract INTRODUCTION: Health planning is required for the control and prevention of severe cases of COVID-19 in children. METHODS: Spatial analysis of severe COVID-19 cases in children of Pernambuco in the first six months of the pandemic and its autocorrelation with the Human Development Index was conducted. RESULTS: A total of 551 severe cases (39.4 cases/100,000 inhabitants) was initially concentrated in the metropolitan area, with later interiorization. The spatial autocorrelation of cases was identified. The bivariate analysis revealed alert regions in less developed municipalities (I=0.341; p=0.001). CONCLUSIONS: Considering the local particularities can assist in directing the priorities for decision making.
DOI: 10.6084/m9.figshare.14277417.v1
title: COVID-19 in children in the state of Pernambuco: Spatial analysis of confirmed severe cases and the Human Development Index

description: Abstract INTRODUCTION: Health planning is required for the control and prevention of severe cases of COVID-19 in children. METHODS: Spatial analysis of severe COVID-19 cases in children of Pernambuco in the first six months of the pandemic and its autocorrelation with the Human Development Index was conducted. RESULTS: A total of 551 severe cases (39.4 cases/100,000 inhabitants) was initially concentrated in the metropolitan area, with later interiorization. The spatial autocorrelation of cases was identified. The bivariate analysis revealed alert regions in less developed municipalities (I=0.341; p=0.001). CONCLUSIONS: Considering the local particularities can assist in directing the priorities for decision making.
DOI: 10.6084/m9.figshare.14277462
title: Trypanosoma cruzi discrete typing unit TcIV implicated in a case of acute Chagas disease in a domiciliated dog in the western Amazon

description: Abstract Chagas disease is caused by the protozoan Trypanosoma cruzi. Seven lineages have been identified based on different molecular markers, namely TcI, TcII, TcIII, TcIV, TcV, TcVI, and TcBat. Dogs play the role of epidemiological sentinels being domestic reservoirs of T. cruzi. The aim of the current study was to report the first case of CD in a domestic dog in Manaus, Amazonas, Brazil, infected with T. cruzi DTU TcIV. We hope our report encourages veterinarians and surveillance professionals to a take a deeper look at T. cruzi infection in domestic animals.
DOI: 10.22008/fk2/oiajvo/1qb6nv
title: diagnostic_retrieval.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14268176
title: Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review

description: Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.
DOI: 10.6084/m9.figshare.14277462.v1
title: Trypanosoma cruzi discrete typing unit TcIV implicated in a case of acute Chagas disease in a domiciliated dog in the western Amazon

description: Abstract Chagas disease is caused by the protozoan Trypanosoma cruzi. Seven lineages have been identified based on different molecular markers, namely TcI, TcII, TcIII, TcIV, TcV, TcVI, and TcBat. Dogs play the role of epidemiological sentinels being domestic reservoirs of T. cruzi. The aim of the current study was to report the first case of CD in a domestic dog in Manaus, Amazonas, Brazil, infected with T. cruzi DTU TcIV. We hope our report encourages veterinarians and surveillance professionals to a take a deeper look at T. cruzi infection in domestic animals.
DOI: 10.6084/m9.figshare.14268176.v1
title: Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review

description: Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.
DOI: 10.6084/m9.figshare.14278673
title: Genotypic selection of multispecific hybrids obtained through crosses between commercial Passiflora edulis and wild passiflora species

description: Abstract The interspecific introgression of resistance genes to diseases in commercial varieties through interspecific crosses has been adopted as a strategy in breeding programs of sour passion fruit. This work aimed to evaluate 11 progenies of multispecific hybrids obtained from crosses involving seven Passiflora species, in addition to four commercial cultivars, using the REML/BLUP method. The experiment was carried out as a randomized block design with six repetitions and three plants per plot. Ten fruit traits were evaluated. The additive, multiplicative, and sum of ranks indexes were applied to determine the most appropriate selective strategy in the simultaneous increase of fruit weight, number of fruits, and yield. Plants from the 325 x VAO progeny showed a lower bacterial defoliation index and better means predicted for the traits number of fruits and productivity. The progenies 325 x LD4, PL3 x LD4, and the controls CSB-Marília and BRS-Gigante Amarelo showed plants with better-predicted means for traits related to fruit quality. The genetic gains demonstrated good prospects for the use of wild species in sour passion fruit genetic breeding.
DOI: 10.6084/m9.figshare.14278673.v1
title: Genotypic selection of multispecific hybrids obtained through crosses between commercial Passiflora edulis and wild passiflora species

description: Abstract The interspecific introgression of resistance genes to diseases in commercial varieties through interspecific crosses has been adopted as a strategy in breeding programs of sour passion fruit. This work aimed to evaluate 11 progenies of multispecific hybrids obtained from crosses involving seven Passiflora species, in addition to four commercial cultivars, using the REML/BLUP method. The experiment was carried out as a randomized block design with six repetitions and three plants per plot. Ten fruit traits were evaluated. The additive, multiplicative, and sum of ranks indexes were applied to determine the most appropriate selective strategy in the simultaneous increase of fruit weight, number of fruits, and yield. Plants from the 325 x VAO progeny showed a lower bacterial defoliation index and better means predicted for the traits number of fruits and productivity. The progenies 325 x LD4, PL3 x LD4, and the controls CSB-Marília and BRS-Gigante Amarelo showed plants with better-predicted means for traits related to fruit quality. The genetic gains demonstrated good prospects for the use of wild species in sour passion fruit genetic breeding.
DOI: 10.6084/m9.figshare.14278681
title: Self-incompatibility characterization in segregating populations of apple trees with DNA markers for S-alleles

description: Abstract The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) cross was characterized as semi-compatible based on DNA markers, and the segregation of the S-alleles in the hybrids was 1:1, as expected. The segregation of the DNA markers occurred together with their respective S-alleles: S2, S3, S5, S10, and S19. Thus, characterization of the S-alleles not only allowed identification of compatibility between parents but also identified contaminations in segregating populations.
DOI: 10.6084/m9.figshare.14278681.v1
title: Self-incompatibility characterization in segregating populations of apple trees with DNA markers for S-alleles

description: Abstract The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) cross was characterized as semi-compatible based on DNA markers, and the segregation of the S-alleles in the hybrids was 1:1, as expected. The segregation of the DNA markers occurred together with their respective S-alleles: S2, S3, S5, S10, and S19. Thus, characterization of the S-alleles not only allowed identification of compatibility between parents but also identified contaminations in segregating populations.
DOI: 10.6084/m9.figshare.14278691
title: Evaluation of the critical points of the most adequate nonlinear model in adjusting growth data of ‘green dwarf’ coconut fruits

description: Abstract ‘Green Dwarf’ coconut is a fruit of great economic interest, since all its components are used, in addition to water, its main component. It is a culture of humid tropics, widely produced in northeastern Brazil, being an important income source for the region. The phenology study of this type of fruit is extremely important, but there are few studies in literature. Regression models, especially nonlinear growth models, can be of great value to understand how fruit growth behaves. The scarcity of works of this nature may be linked to some difficulties in estimating parameters of nonlinear models, such as assigning initial values to the itterative process. Overcoming this difficulty, for regression analysis, linear or not, several steps need to be respected to ensure the validity of information. Much information can be extracted from nonlinear growth models, such as the asynotic value, growth rate and critical points (maximum acceleration point, inflection point, maximum deceleration point and asynotic deceleration point). The aim of this work was to describe the stages of nonlinear regression analysis and to estimate the critical points of ‘Green Dwarf ’ coconut growth curves. After initial adjustments, the only unmet assumption was independence, adding a first order autoregressive term. Again, models were adjusted and all parameters were significant, with both models, Gompertz and Logistic, adjusting well to data, with slight advantage for the Logistic model with better adjustment quality criteria values, with maximum expected LED and LEDKP values of 21.4037 cm and 21.5478 cm, respectively. The x and y axis of critical points were estimated, with values that can help producers to make more objective decisions about the appropriate time to harvest coconut fruits, considering the most diverse uses of this type of fruit.
DOI: 10.6084/m9.figshare.14279110
title: PROPERTIES OF SILICON CARBIDE THERMAL PROTECTION IN CERAMIC COATINGS

description: In recent years, research on the development of protective materials has expanded significantly in order to find efficient and economically viable solutions for application in various industrial segments. Among these materials, those aimed at protecting metallic substrates against high temperatures that end up causing corrosive processes, stand out due to the huge market demand. Advantages such as reproducibility in the process, cost reduction and reduction in environmental impact also directly contribute to the search for protective coatings as an efficient protective alternative. This article presents the characterization of a ceramic coating developed in a previous study that used silicon carbide as a filler, using potassium silicate (water glass) as a matrix (binder), and whose anti-corrosion and thermal protection properties stood out, mainly in applications in the aerospace industry. In this stage of the work, tests of thermal analysis (thermogravimetry) were carried out for the physical and chemical characterization of the material. The results corroborate those previously achieved when applying the coating on components of a hybrid rocket engine tested on a fixed-point bench.
DOI: 10.6084/m9.figshare.14278678
title: Collection time, dehidration, culture media and environment for germination and storage of campomanesia guazumifolia (cambess.) O. Berg. Pollen

description: Abstract Sete-capote tree, a neglected species, has great ecological, cultural and potential economic importance, however, studies for this and other native species of the Myrtaceae family are still scarce. The objective of this study was to elucidate aspects of pollen germination and viability of this specie. For the experiment, pollen from flowers in pre and post anthesis was used, which was dehydrated in a chamber containing silica, for different periods. For germination tests, different concentrations of sucrose, boric acid and calcium nitrate were used. After obtaining germination results above 80%, the pollen grains were stored in refrigerator (5 °C), freezer (-17 °C), liquid nitrogen (-147 °C) and natural environment (± 25 °C), evaluating monthly the germination, until total loss of viability. For germination, it was recommend using pollen from flowers in post-anthesis, dehydrated for 24 hours in a silica chamber. The culture medium should contain 12% sucrose (C12H22O11), 10% boric acid (H3BO3) and 20% calcium nitrate (Ca(NO3)2) to obtain high germinative percentages. In addition, pollen presents orthodox behavior and when stored in liquid nitrogen, remains viable for 30 days.
DOI: 10.6084/m9.figshare.14278678.v1
title: Collection time, dehidration, culture media and environment for germination and storage of campomanesia guazumifolia (cambess.) O. Berg. Pollen

description: Abstract Sete-capote tree, a neglected species, has great ecological, cultural and potential economic importance, however, studies for this and other native species of the Myrtaceae family are still scarce. The objective of this study was to elucidate aspects of pollen germination and viability of this specie. For the experiment, pollen from flowers in pre and post anthesis was used, which was dehydrated in a chamber containing silica, for different periods. For germination tests, different concentrations of sucrose, boric acid and calcium nitrate were used. After obtaining germination results above 80%, the pollen grains were stored in refrigerator (5 °C), freezer (-17 °C), liquid nitrogen (-147 °C) and natural environment (± 25 °C), evaluating monthly the germination, until total loss of viability. For germination, it was recommend using pollen from flowers in post-anthesis, dehydrated for 24 hours in a silica chamber. The culture medium should contain 12% sucrose (C12H22O11), 10% boric acid (H3BO3) and 20% calcium nitrate (Ca(NO3)2) to obtain high germinative percentages. In addition, pollen presents orthodox behavior and when stored in liquid nitrogen, remains viable for 30 days.
DOI: 10.22008/fk2/oiajvo/qx0uod
title: grain_diameter.tif

description: SICE version: Cloud removed, PPGC, SNAP7, pySICEv1.4
DOI: 10.6084/m9.figshare.14278691.v1
title: Evaluation of the critical points of the most adequate nonlinear model in adjusting growth data of ‘green dwarf’ coconut fruits

description: Abstract ‘Green Dwarf’ coconut is a fruit of great economic interest, since all its components are used, in addition to water, its main component. It is a culture of humid tropics, widely produced in northeastern Brazil, being an important income source for the region. The phenology study of this type of fruit is extremely important, but there are few studies in literature. Regression models, especially nonlinear growth models, can be of great value to understand how fruit growth behaves. The scarcity of works of this nature may be linked to some difficulties in estimating parameters of nonlinear models, such as assigning initial values to the itterative process. Overcoming this difficulty, for regression analysis, linear or not, several steps need to be respected to ensure the validity of information. Much information can be extracted from nonlinear growth models, such as the asynotic value, growth rate and critical points (maximum acceleration point, inflection point, maximum deceleration point and asynotic deceleration point). The aim of this work was to describe the stages of nonlinear regression analysis and to estimate the critical points of ‘Green Dwarf ’ coconut growth curves. After initial adjustments, the only unmet assumption was independence, adding a first order autoregressive term. Again, models were adjusted and all parameters were significant, with both models, Gompertz and Logistic, adjusting well to data, with slight advantage for the Logistic model with better adjustment quality criteria values, with maximum expected LED and LEDKP values of 21.4037 cm and 21.5478 cm, respectively. The x and y axis of critical points were estimated, with values that can help producers to make more objective decisions about the appropriate time to harvest coconut fruits, considering the most diverse uses of this type of fruit.