cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2015.425
title: Estrogenic activity and mechanism of action of puerarin phytoestrogen on mouse osteoclast

description: The effects and mechanisms of action of Pueraria mirifica extract (PME) and puerarin (PU) on primary mouse osteoclasts and cross-talk between osteoblasts and osteoclasts were investigated. Three experimental series were set up. Experiment I: to study the effects of PME and PU on anti-osteoclastogenesis, mouse bone marrow macrophages (BMMs) were incubated with 0.3% DMSO, 1, 5 and 10 mg/ml PME, 0.1, 10 and 1000 nM PU, and 10 nM E2 for 24 h, and cell proliferation, differentiation, fusion and resorptive function were determined. PME and PU suppressed the RANKL-induced differentiation of BMMs into pre-osteoclasts and the fusion of pre-osteoclasts to multi-nucleated mature osteoclasts. The PME showed a differential dose-response effect, where a low PME dose (1 mg/ml) suppressed the fusion stage of mononuclear pre-osteoclast and a high PME dose (10 mg/ml) suppressed the differentiation from BMMs to pre-osteoclasts and decreased mRNA expression levels of Nfatc1 and Tm7sf4. The mechanisms of the low PME dose and PU were passed through estrogen receptors (ERs). Nevertheless, the anti-osteoclastogenic activity of a high PME dose was not significantly suppressed by ER antagonists, suggesting that PME might act via one or more alternative pathway(s). Experiment II: to study the effects of PME and PU on induction of osteoclast apoptosis, BMMs were treated with 0.3% DMSO, 10 nM E2, 1 and 10 mg/ml PME and 1000 nM PU for 72h, and cell apoptosis was determined. PME, but not PU and E2, induced apoptosis in mouse BMMs-preosteoclasts, while FasL mRNA expression levels were decreased after 24-h exposure to all three treatments. Experiment III: to study the effects of PME and PU on the cross-talk between osteoblasts and osteoclasts, each single-cell type culture (either mouse osteoblast ST2 precursors or BMMs) and a two-cell type coculture condition were conducted. Cells were treated with 0.025% DMSO, 10 nM E2, 1 mg/ml PME and 1000 nM PU. Exposure of ST2 cells alone with the PME reduced Rankl expression, which led to a significant decrease in Rankl/Opg ratio. However, exposure of BMMs alone or two-cell type coculture with PME, PU and E2 had no effects on expression of Rankl, Opg, Rankl/Opg ratio, Rank, EphB4 and EfnB2. In conclusion, PME and PU elicit the anti-osteoclastogenic activity of both directly on osteoclasts and indirectly via the regulation of osteoblasts through RANKL/OPG/RANK signal This study increases the knowledge and understanding of the mode of action of PME and PU on bone cells which corroborates the high potential of PME and PU to be developed as anti-resorptive agents for osteoporotic patients.
DOI: 10.14457/cu.the.2015.426
title: Electrochemically reduced micellar graphene oxide modified electrode for simultaneous detection of carbofuran and carbendazim

description: Graphene, an isolated single layer of graphite, is one of the well-publicized carbon based nanomaterials used for electrode modification in electrochemical sensor due to its outstanding electrochemical properties. However, hydrophobicity of graphene makes it incompatible with aqueous solution, leading to impediment to electron transfer process. Here, we alter graphene property to be hydrophilicity by using electrochemically reduced micellar graphene oxide for electrode surface modification. Then, this system was applied for the simultaneous determination of carbofuran and carbendazim. Interestingly, the modified electrode offers an improved electrochemical sensitivity, verified by a drastic increase in current signal of carbofuran (4 times) and carbendazim (12 times) compared with unmodified electrode. Under the optimal conditions, low detection limits of carbofuran and carbendazim were found to be 10 µg L-1 and 5 µg L-1 with the wide linear ranges of 40-20,000 µg L-1and 25-5,000 µg L-1, respectively. Ultimately, this approach was successfully applied for sensitive determination of carbofuran and carbendazim residues in real agricultural products.
DOI: 10.14457/cu.the.2015.427
title: Taxonomy of nocturnal parasitic wasps family Braconidae at Samaesan Islands, Chonburi Province

description: Parasitic wasps are classified in the order Hymenoptera, same group as bees, ants and other wasps. Braconid wasps belong to the extremely diverse superfamily Ichneumonoidea. The characteristics of the braconid wasps are (1) fore wing without vein 2m-cu and/or with 1/Rs+M, (2) hind wing with 1r-m arising basal to split between R and RS, and (3) second and third metasomal tergites fusied. The aims of this research are to study the taxonomy of the nocturnal parasitic wasps, belonging to the family Braconidae on the Samaesan Islands and from the surrounding areas and construct the pictorial key of the parasitic wasp in the family Braconidae in subfamily level, including created the database of nocturnal braconids recorded from the study sites. In this study, 2 types of traps: black light trap and mobile bucket light traps were used to collect the specimens during September 2013 to September 2014. One hundred and seventy five morphospecies within 17 subfamilies (12 koinobionts and 5 idibionts) from 652 specimens have been collected and number of koinobiont specimens has been recorded more than the idiobionts in most of the collecting periods. A new record of Braconid subfamilies in Thailand have been reported for first time: Helconinae, Lysiterminae, Pambolinae, and Rhysipolinae. Most of the specimens are Cheloninae representing about 45% of all specimens (291 from 652 specimens) and the highest number of morphospecies belongs to the subfamily Cheloninae. Number of braconid species collecting from Khao Ma Cho is similar to those of Samaesan Island. Estimated species richness of the braconid species by Chao-1 in the study sites is approximately 271 species. Moreover, a new species of braconid wasp in the genus Aleiodes Wesmael, 1838 was also discovered in this study.
DOI: 10.14457/cu.the.2015.428
title: Optimization for concomitant production and partial purification of protease and lipase from Bacillus licheniformis 3C5 in aqueous two-phase system

description: An organic solvent-, detergent-tolerant Bacillus licheniformis 3C5 has been recognized as protease-producing bacterium. Further tests revealed that this strain could also secrete proteolytic-resistant lipase with good stability in organic solvent and detergent and thus it is suitable for industrial co-application with protease. Further optimization studies for enhancing concomitant protease and lipase production were performed using response surface methodology (RSM). The maximum protease and lipase production was achieved using the optimized medium containing 0.86% glucose, 0.6% yeast extract, 0.25% NaCl and 2.5% olive oil (w/v), and led to an increase in yield by 2.20 and 12.57 folds. Finally, protease and lipase were partially purified by using an aqueous-two phase system (ATPS). The RSM was also applied to investigate the concentration effects of PEG6000, sodium citrate and KCl on the partition of both enzymes. It was found that protease was preferentially partitioned into PEG-rich top phase, whereas lipase was distributed to the interface. Accordingly, the best partitioning system was composed of 18.58% (w/w) PEG6000, 8.0% (w/w) sodium citrate and 4.0% (w/w) KCl. It provided 93.18% yield of protease and 92.59% yield of lipase with purification fold of 10.58 and 14.58, respectively.
DOI: 10.14457/cu.the.2015.429
title: Development of 8-hydroxy-9-iminyljulolidine derivatives as fluorescent sensors

description: The development of fluorescent sensor which is specify toward metal ion become important. In this work, the fluorescent sensor containing julolidine derivative as a fluorophore linked with the control part using of dipicolylamine derivative substituted on aniline ring at ortho- (J2P), meta- (J3P) and para- (J4P) position, respectively, were successfully developed and synthesized via Schiff base reaction. According to the experimental results under the mixed solvent condition of H2O/methanol (1:9, v/v), the maximum absorption bands of compounds J2P, J3P and J4P were observed at 380, 415 and 420 nm, respectively. Moreover, in the presence of aluminum ion (Al3+), the fluorescent signals of compound J2P significantly enhanced. The maximum emission intensity appeared at 490 nm along with the fluorescence quantum yield as 0.156. The association constant (Ka) was calculated as 2.25 x 105 M-1. Furthermore, when the solvent condition was changed to H2O/DMSO (5:95, v/v), compound J2P provided significant response toward only magnesium ion (Mg2+). The strongest fluorescent signal was observed at 470 nm with the fluorescence quantum yield as 0.096. The Ka value of the coordination between J2P and Mg2+ is 4.00 x 104 M-1. The stoichiometric complexation between J2P ligand and both metal ion supported the formation of 1:1 and the detection limits (LOD) for Al3+ and Mg2+ detection under above conditions were 0.17 µM and 1.32 µM, respectively. In addition, the fluorescence enhancing phenomenon was extrapolated to occur through the Chelation-enhanced fluorescence (CHEF) mechanism.
DOI: 10.14457/cu.the.2015.430
title: Theoretical study of catalytic cracking of C5–C6 alkanes to olefins using zeolites

description: The cracking mechanisms of n-pentane and n-hexane over the H-ZSM-5 and H-FER catalysts were investigated using the ONIOM(B3LYP/6-31+G(d,p):AM1) method with 52T and 64T cluster model, respectively. The computation results show that the cracking process of n-pentane and n-hexane over the H-ZSM-5 and H-FER catalysts consisting of two reaction paths. The first path is turnover path. The second path is poisoning path. The cracking processes of the n-pentane and n-hexane over either the H-ZSM-5 or H-FER catalyst afford the 1,2-pentene and 1,2-hexene as the major products and then provide 2-pentoxy and 2-hexoxy species as catalyst poisoning, respectively. Thermodynamic quantities, rate constants, equilibrium constants and reaction energies of cracking reaction of n-pentane and n-hexane over the H-ZSM-5 and H-FER catalysts are reported.
DOI: 10.14457/cu.the.2015.431
title: Encapsulation of gibberellic acid by hydrophobic modified starch for accelerating growth of plants

description: The objective of this research is to encapsulate of gibberellic acid (GA3) by hydrophobic modified starch (HB-Starch). It was prepared by octadecyl bromide conjugation. The HB part was able to perform self-assembled nano micelles which used in agricultural applications for the controlled release. The molecular structure, surface morphology and particle size were characterized by 1H-NMR, FTIR, SEM and zetasizer analysis techniques. Evidently, the degree of octadecyl chain substitution was 8.5 % calculated by 1H-NMR. The evidences illustrated that the octadecyl chain was successfully conjugated on the starch backbone and provided the hydrophobic properties. Additionally, the CMC value of micelle was 0.25 µg/mL determined by fluorescence technique. Furthermore, 1% (w/w) of GA3-HB-Starch produced high entrapment efficiency as well as prolonged the release than the pure GA3, which this led to an increase of plant growth acceleration at the optimum concentration (0.5 ppm).
DOI: 10.14457/cu.the.2015.432
title: One-pass-throw-away learning of temporal class-shift by multi-stratum network

description: The problem of learning streaming non-expired and temporally expired data occurring in various business applications was studied. If there exists a class whose all data are eventually expired with some unknown reasons, then the relevant neurons and their links must be entirely removed. A new learning based on dynamic network structure called Multi-Stratum Network Learning (MSNL) was proposed to cope with this problem of data life change. Furthermore, to speed up the learning time and to maintain a minimum space complexity for streaming data, the new concept of one-pass-throw-away learning in forms of recursive functions for handling the expiration class was introduced. The experimental results signified that the proposed algorithm outperformed the other incremental-like learning algorithms in terms of time and space complexities.
DOI: 10.14457/cu.the.2015.433
title: Synthesis of novel truxene derivatives as chemosensors for nitroaromatics and metal ions

description: Two fluorescent compounds with truxene as a fluorophore are synthesized and developed as turn-off fluorescent sensors for nitroaromatic compounds (T1) and copper(II) ion (T2). Six hydrophobic n-butyl groups in T1 and six hydrophilic diglycol chains in T2 prevent pi-pi stacking of truxene core and enhance their solubilities in organic or aqueous media. The synthesis of each compound is based primarily on the Sonogashira coupling reactions, which produced the desired compounds T1 and T2 in the overall yields of 14 and 18 %, respectively. The photophysical and sensing properties of T1 depend on the solvents. In chloroform, T1 is selectively quenched by 2-nitrophenol. However, the quenching is the fluorescence signal of selectively observed with picric acid in aqueous tetrahydrofuran. The detection limits are determined as 1.54 and 0.15 ppm for 2-nitrophenol and picric acid, respectively. The considerable overlapping of the absorption spectra of T1 and that of the analytes, along with the correlation of the fraction of photons absorbed by T1 and quenching efficiencies, the sensing mechanism significantly involves inner-filter effect, where the analytes compete with T1 during the absorption process. However, the lower quenching efficiency at higher temperature suggested that energy transfer via static quenching may also involve.Compound T2 can dissolve well in tetrahydrofuran containing up to 70% of water and shows effective and selective fluorescence quenching towards copper(II) ion without any interference from other metal ions. The detection limit of this sensing system is 0.06 ppm. The mass analysis using a mixture between T2 and copper(II) ion corresponds to the mass of a 1:1 complex between T2 and copper(II), as verified by a Job’s plot. Thus, the quenching mechanism should involves the formation of such non fluorescent ground state complex. In addition, an anion sensing system is developed from the complex of T2 and copper(II) ion, which shows fluorescence recovery upon the addition of hydrogen phosphate and several biological phosphate compounds. The signal restoration involves the coordination of phosphate group to copper(II) preventing the complexation of copper(II) ion with T2.
DOI: 10.14457/cu.the.2015.434
title: Configuration design of soda-lime glass laminated transparent armor by finite element analysis

description: The goal of this research is to design a lighter weight soda-lime glass/polyvinyl butyral (PVB) laminated transparent armor capable of NIJ level III ballistic protection by finite element analysis (FEA) technique. The armor and projectile with impact velocity at 853 m/s was modeled in 3D using quarter symmetry. The meshing parameters and material model were calibrated to obtain the reliable accuracy in FEA results.The effects of the striking glass thickness (19, 15, 12, 10, 8, 6 and 3 mm), PVB film (0.76 and 1.52 mm) thicknesses/ordering and the glass/PVB film configurations with reduced total target thickness on the ballistic performance were systematically examined. The FEA results were analyzed and compared to ballistic testing results in terms of the volumetric damage, the distribution of internal and kinetic energy in the laminates, the depth of penetration, and the crater diameter. These results will be subsequently used to evaluate the ballistic performance of laminated armor designs and to identify the optimal design for lighter weight laminated armor as compared to the reference design.Lastly, the thinnest and lightest-weight soda-lime glass/PVB laminated transparent armor in this study could reach 13.13% and 13.62% of thickness and weight reductions, respectively, comparing to the commercial models. Thus, it could indicate that the FEA performed using ANSYS explicitSTR® software is a powerful tool for the study of the ballistic impact process, the damage propagation, as well as in optimizing the design of laminated transparent armor systems.
DOI: 10.14457/cu.the.2015.435
title: pH-responsive polyphenol-zinc oxide hybrid nanoparticles for anticancer drug delivery

description: Organic-inorganic hybrid nanomaterial fabricated by the interactions between organic and inorganic matters has attracted great deal of attention for the introduction of novel functions. Zinc oxide nanoparticles show many biological activities such as anticancer and antibacterial. The tea polyphenol has been reported as the potential anticancer agent. In this work, we interested in combination of tea polyphenol and zinc oxide as the organic-inorganic hybrid nanomaterial for delivery of antitumor agents. Herein, we have developed drug delivery system from the complexation of zinc oxide and natural product extract. We showed the particles fabrication, characterization and drug delivery application of this organic-inorganic hybrid material. Delivering of drugs (doxorubicin and paclitaxel) by the tea polyphenol-ZnO nanoparticles into cancerous cells was achieved. We also verified that the particles can be taken up into the cancer cell by endocytosis mechanism and endosome burst to release chemicals to cytosol as the mechanism of releasing of the particles.
DOI: 10.6084/m9.figshare.13646771
title: GM data of South China.rar

description: 1.SCBouguergrav.grd. Data of figure 2,It’s the Bouguer gravity anomalies of South China calculated form satellite gravity data under spherical coordinates.2.SCEMAG2RTP.grd. Data of figure 2,It’s the reduction to the pole magnetic anomalies of South China based on EMAG2 by moving windows(using different geomagnetic parameters in differnt windows)
DOI: 10.17632/rd6rm8zpfp.1
title: Data for: Electrons Irradiation of III-V//Si Solar Cells for NIRT Conditions

description: Solar cells characterization at BOL and EOL. PC1D simulation parameters.
DOI: 10.14457/cu.the.1987.104
title: พฤติกรรมกล้าแสดงออกของนักศึกษาวิทยาลัยพลศึกษาตามการรับรู้ของตนเอง
DOI: 10.5281/zenodo.3735329
title: Integrated spatial genomics dataset

description: Supplementary processed data. The raw microscopy data are not uploaded owing to their large size (2.9 Tb), but are available upon reasonable request (Long Cai: lcai@caltech.edu). These supplementary processed data contain additional files for RNA seqFISH, DNA seqFISH+ and sequential immunofluorescence. DNAseqFISH+.zip contains DNA seqFISH+ pixel locations for 3,600 loci in single cells (pixel sizes are 103 nm for x and y, and 250 nm for z). Please refer to Supplementary Table 1 at the journal website for genomic coordinates of the targeted loci. RNA seqFISH.zip contains RNA seqFISH expression profiles for mRNAs and introns in single cells. Intron data also contain pixel coordinates for intron spot locations. Cells were filtered out if keep1 column is 0 due to the laser illumination bias. Excel file contains additional information for sequential immunofluorescence experiments. The file contains target names with corresponding hybridization cycle round and fluorescent channel information per each experiment. Zip files for IF binned data contain IF intensity information in each pixel coordinate (either bin1 or bin2). Please refer to the included excel file to match each target to each hybridization and fluorescent channel.
DOI: 10.5281/zenodo.3735328
title: Integrated spatial genomics dataset

description: Supplementary processed data. The raw microscopy data are not uploaded owing to their large size (2.9 Tb), but are available upon reasonable request (Long Cai: lcai@caltech.edu). These supplementary processed data contain additional files for RNA seqFISH, DNA seqFISH+ and sequential immunofluorescence. DNAseqFISH+.zip contains DNA seqFISH+ pixel locations for 3,600 loci in single cells (pixel sizes are 103 nm for x and y, and 250 nm for z). Please refer to Supplementary Table 1 at the journal website for genomic coordinates of the targeted loci. RNA seqFISH.zip contains RNA seqFISH expression profiles for mRNAs and introns in single cells. Intron data also contain pixel coordinates for intron spot locations. Cells were filtered out if keep1 column is 0 due to the laser illumination bias. Excel file contains additional information for sequential immunofluorescence experiments. The file contains target names with corresponding hybridization cycle round and fluorescent channel information per each experiment. Zip files for IF binned data contain IF intensity information in each pixel coordinate (either bin1 or bin2). Please refer to the included excel file to match each target to each hybridization and fluorescent channel.
DOI: 10.5281/zenodo.4470262
title: Data files to support reproducing analyses in "Overcoming bias in gene category enrichment analyses of brain-wide transcriptomic data"

description: A repository of raw and processed data to accompany the manuscript, 'Overcoming bias in gene category enrichment analyses of brain-wide transcriptomic data'. A description of how these files can be used to reproduce analyses is in the documentation for the code in this repository https://github.com/benfulcher/GCEA_FalsePositives
description: {"references": ["Fulcher et al., Overcoming bias in gene-set enrichment analyses of brain-wide transcriptomic data. bioRxiv. https://doi.org/10.1101/2020.04.24.058958"]}
DOI: 10.6084/m9.figshare.13646930
title: Additional file 2 of Preoperative risk factors including serum levels of potassium, sodium, and creatinine for early mortality after open abdominal surgery: a retrospective cohort study

description: Additional file 2. Predictive model for 30 days mortality in in patients undergoing open abdominal surgery.
DOI: 10.6084/m9.figshare.13646956
title: Additional file 1 of Patterns of variation of mutation rates of mitochondrial and nuclear genes of gastropods

description: Additional file 1: Table S1. Information on PopSet data analyzed in this study.
DOI: 10.6084/m9.figshare.12872312.v1
title: Additional file 6 of The fecal microbiota of wild and captive raptors

description: Additional file 6: Table S2. Metadata of 46 raptors included in the study.
DOI: 10.6084/m9.figshare.12872309.v1
title: Additional file 5 of The fecal microbiota of wild and captive raptors

description: Additional file 5: Table S1. Taxonomic classification of 16S sequences from 46 fecal microbiota.
DOI: 10.6084/m9.figshare.12872312
title: Additional file 6 of The fecal microbiota of wild and captive raptors

description: Additional file 6: Table S2. Metadata of 46 raptors included in the study.
DOI: 10.6084/m9.figshare.12872309
title: Additional file 5 of The fecal microbiota of wild and captive raptors

description: Additional file 5: Table S1. Taxonomic classification of 16S sequences from 46 fecal microbiota.
DOI: 10.6084/m9.figshare.13646990
title: Additional file 10 of Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

description: Additional file 10. Taxonomic profile of human fecal samples from MinION™ sequencing (amplicons: V3-V4).
DOI: 10.6084/m9.figshare.13646993
title: Additional file 11 of Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

description: Additional file 11. Taxonomic profile of human fecal samples from MiSeq™ sequencing (amplicons: V3-V4).
DOI: 10.6084/m9.figshare.13646996
title: Additional file 12 of Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

description: Additional file 12. Taxonomic profiles of human fecal samples from MiSeq™ sequencing (amplicons: V3-V4, taxonomic classification by OTU-based analysis using the QIIME 2 pipeline).