cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19963566.v1
title: Renal inflammatory and oxidative and metabolic changes after 6 weeks of cafeteria diet in rats

description: Abstract Introduction: Obesity is a disease in which inflammation is directly involved and can lead to impaired renal function. Objective: To evaluate the influence of short term exposure to cafeteria diet on kidney tissue inflammation and advanced glycation end products (AGEs) in the rat plasma. Methods: Male Wistar rats (10 weeks of age, weighing 350 g) were assigned to receive commercial chow diet (C; n = 8 animals/group, 5% of energy from fat) or cafeteria diet (CAF-D, n = 8 animals/group: 29% energy fat) and sucrose in drinking water (300 g/L) for 6 weeks. Results: adiposity index at six weeks was higher in CAF-D group compared to C. The same behavior was observed for plasma levels of glucose, triglycerides, leptin, insulin and AGEs. The gene expression of IL-6 and TNF-α in renal tissue was higher in CAF-D group and no significant difference in adipose tissue. There was no increase of these cytokines in plasma and kidney or histologically. There was a significant decrease of adiponectin in the CAF-D group. Conclusion: The short exposure CAF-D reflects changes in metabolism, increased plasma levels of AGEs, which may reflect the increased expression of inflammatory cytokines in the kidney.
DOI: 10.6084/m9.figshare.19963566
title: Renal inflammatory and oxidative and metabolic changes after 6 weeks of cafeteria diet in rats

description: Abstract Introduction: Obesity is a disease in which inflammation is directly involved and can lead to impaired renal function. Objective: To evaluate the influence of short term exposure to cafeteria diet on kidney tissue inflammation and advanced glycation end products (AGEs) in the rat plasma. Methods: Male Wistar rats (10 weeks of age, weighing 350 g) were assigned to receive commercial chow diet (C; n = 8 animals/group, 5% of energy from fat) or cafeteria diet (CAF-D, n = 8 animals/group: 29% energy fat) and sucrose in drinking water (300 g/L) for 6 weeks. Results: adiposity index at six weeks was higher in CAF-D group compared to C. The same behavior was observed for plasma levels of glucose, triglycerides, leptin, insulin and AGEs. The gene expression of IL-6 and TNF-α in renal tissue was higher in CAF-D group and no significant difference in adipose tissue. There was no increase of these cytokines in plasma and kidney or histologically. There was a significant decrease of adiponectin in the CAF-D group. Conclusion: The short exposure CAF-D reflects changes in metabolism, increased plasma levels of AGEs, which may reflect the increased expression of inflammatory cytokines in the kidney.
DOI: 10.6084/m9.figshare.19963583
title: Creatinine measurement on dry blood spot sample for chronic kidney disease screening

description: Abstract Introduction: Chronic kidney disease (CKD) screening is advisable due to its high morbidity and mortality and is usually performed by sampling blood and urine. Objective: Here we present an innovative and simpler method, by measuring creatinine on a dry blood spot on filter paper. Methods: One-hundred and six individuals at high risk for CKD were enrolled. The creatinine values obtained using both tests and the demographic data of each participant allowed us to determinate the eGFR. The adopted cutoff for CKD was an eGFR &lt; 60 ml/min. Results: Mean age was 57 ± 12 years, 74% were female, 40% white, and 60% non-white. Seventy-six percent were hypertensive, 30% diabetic, 37% had family history of CKD, and 22% of smoking. The BMI was 29.5 ± 6.9 kg/m2, median systolic blood pressure was 125 mmHg (IQR 120-140 mmHg) and median diastolic blood pressure was 80 mmHg (IQR 70-80 mmHg). According to MDRD equation, sensitivity was 96%, specificity 55%, predictive positive value 96%, predictive negative value 55% and accuracy 92%. By the CKD-EPI equation the sensitivity was 94%, specificity 55%, predictive positive value 94%, predictive negative value 55% and accuracy 90%. A Bland and Altman analysis showed a relatively narrow range of creatinine values differences (+ 0.68mg/dl to -0.55mg/dl) inside the ± 1.96 SD, without systematic differences. Conclusion: Measurement of creatinine on dry blood sample is an easily feasible non-invasive diagnostic test with good accuracy that may be useful to screen chronic kidney disease.
DOI: 10.6084/m9.figshare.19963583.v1
title: Creatinine measurement on dry blood spot sample for chronic kidney disease screening

description: Abstract Introduction: Chronic kidney disease (CKD) screening is advisable due to its high morbidity and mortality and is usually performed by sampling blood and urine. Objective: Here we present an innovative and simpler method, by measuring creatinine on a dry blood spot on filter paper. Methods: One-hundred and six individuals at high risk for CKD were enrolled. The creatinine values obtained using both tests and the demographic data of each participant allowed us to determinate the eGFR. The adopted cutoff for CKD was an eGFR &lt; 60 ml/min. Results: Mean age was 57 ± 12 years, 74% were female, 40% white, and 60% non-white. Seventy-six percent were hypertensive, 30% diabetic, 37% had family history of CKD, and 22% of smoking. The BMI was 29.5 ± 6.9 kg/m2, median systolic blood pressure was 125 mmHg (IQR 120-140 mmHg) and median diastolic blood pressure was 80 mmHg (IQR 70-80 mmHg). According to MDRD equation, sensitivity was 96%, specificity 55%, predictive positive value 96%, predictive negative value 55% and accuracy 92%. By the CKD-EPI equation the sensitivity was 94%, specificity 55%, predictive positive value 94%, predictive negative value 55% and accuracy 90%. A Bland and Altman analysis showed a relatively narrow range of creatinine values differences (+ 0.68mg/dl to -0.55mg/dl) inside the ± 1.96 SD, without systematic differences. Conclusion: Measurement of creatinine on dry blood sample is an easily feasible non-invasive diagnostic test with good accuracy that may be useful to screen chronic kidney disease.
DOI: 10.6084/m9.figshare.19963590
title: Prevalence of chronic renal disease in adults attended by the family health strategy

description: Abstract Introduction: Chronic Kidney Disease (CKD) is an important Brazilian public health issue that has as main etiologies, arterial hypertension and diabetes mellitus (DM). The precocious diagnosis is important, because it allows the implementation of preventive measures that retard or interrupt the progression to the most advanced stages of the CKD. Objective: Identify the prevalence and the associated factors to the CKD among adults served by the Family Health Strategy (FHS). Methods: Cross-sectional study with epidemiological, descriptive and observational design, realized with 511 adults older than 20 years, served by the FSH in a region of Goiania. CKD was defined as GFR &lt; 60 mL/min/1.73m2 and/or albuminuria ≥ 30 mg/g. The GFR was estimated by the Cockcroft-Gault equation and albuminuria by the ratio of albumin and urinary creatinine in the urine sample. The independent variables were age, sex, blood pressure, alcohol comsumption, DM, smoking and overweight/obesity. Results: The CKD prevalence was 32,53%. While GFR &lt; 60ml/min/1.73 m2 occurred in 10,64% and albuminuria in 25,29% of the sample. The analysis identified a significant association between the ages ≥ 60 and GFR &lt; 60ml/min/1.73 m2 (p &lt; 0,001); as the albuminuria ≥ 30 mg/g the association was found in the male gender (p = 0,043), DM (p = 0,002) and alcohol consumption (p = 0,035). Conclusion: There was a high prevalence of CKD's early stages on FHS, taking in consideration the risk factors of age ≥ 60 years old, masculine gender, DM and alcohol consume. Therefore, a CKD screening and monitoring is suggested in adults who are served by the FHS.
DOI: 10.6084/m9.figshare.19963590.v1
title: Prevalence of chronic renal disease in adults attended by the family health strategy

description: Abstract Introduction: Chronic Kidney Disease (CKD) is an important Brazilian public health issue that has as main etiologies, arterial hypertension and diabetes mellitus (DM). The precocious diagnosis is important, because it allows the implementation of preventive measures that retard or interrupt the progression to the most advanced stages of the CKD. Objective: Identify the prevalence and the associated factors to the CKD among adults served by the Family Health Strategy (FHS). Methods: Cross-sectional study with epidemiological, descriptive and observational design, realized with 511 adults older than 20 years, served by the FSH in a region of Goiania. CKD was defined as GFR &lt; 60 mL/min/1.73m2 and/or albuminuria ≥ 30 mg/g. The GFR was estimated by the Cockcroft-Gault equation and albuminuria by the ratio of albumin and urinary creatinine in the urine sample. The independent variables were age, sex, blood pressure, alcohol comsumption, DM, smoking and overweight/obesity. Results: The CKD prevalence was 32,53%. While GFR &lt; 60ml/min/1.73 m2 occurred in 10,64% and albuminuria in 25,29% of the sample. The analysis identified a significant association between the ages ≥ 60 and GFR &lt; 60ml/min/1.73 m2 (p &lt; 0,001); as the albuminuria ≥ 30 mg/g the association was found in the male gender (p = 0,043), DM (p = 0,002) and alcohol consumption (p = 0,035). Conclusion: There was a high prevalence of CKD's early stages on FHS, taking in consideration the risk factors of age ≥ 60 years old, masculine gender, DM and alcohol consume. Therefore, a CKD screening and monitoring is suggested in adults who are served by the FHS.
DOI: 10.6084/m9.figshare.19963605.v1
title: Frequency and clinical histological analysis of glomerular diseases in a tertiary hospital in southern Brazil

description: Resumo Introduction: The glomerulopathies are the most common biopsy-proven kidney diseases. The epidemiological investigation of glomerulopathies allows the identification of their distribution and main causes and enables the development of prevention and treatment strategies. Objective: This study aims to identify the frequency and clinical-pathological correlation of glomerular diseases diagnosed at the HC-UFPR over the period of 5 years. Methods: 131 biopsies were performed between January 1, 2008 and December 31, 2012 and were analysed by light and immunofluorescence microscopy. Histopathological slides were reviewed by a pathologist. Clinical and laboratory data and the immunofluorescence microscopy results were extracted from medical records. The findings were tabulated and analysed. Results: 128 of 131 cases were reanalysed. 46.5% were obtained from men. Patients' age averaged 43 years for men and 38 for women. In 99 cases, the indication of biopsy was identified; 49.5% cases presented nephrotic syndrome, 17.17%, acute renal failure and 15.15%, chronic renal failure; 8.08%, nephritic syndrome; 6.06%, isolated proteinuria and 4.04% isolated hematuria. In 61.21% an underlying disease related to the glomerulopathy could be identified; 33.62% corresponded to primary disease and in 5.17% of cases the nature of the glomerulopathy could not be determined. Among secondary glomerulopathies, the most frequent was Lupus Nephritis (49.29%), and among the primary, Focal Segmental Glomerulosclerosis (30.77%) and Membranous Nephropathy (25.64%). Conclusion: The average patient with glomerulopathy in this service is an adult with nephrotic syndrome. Unlike other reports, secondary glomerulopathies were predominant. These findings may reflect the tertiary characteristic of the assistance at HC-UFPR.
DOI: 10.6084/m9.figshare.19963605
title: Frequency and clinical histological analysis of glomerular diseases in a tertiary hospital in southern Brazil

description: Resumo Introduction: The glomerulopathies are the most common biopsy-proven kidney diseases. The epidemiological investigation of glomerulopathies allows the identification of their distribution and main causes and enables the development of prevention and treatment strategies. Objective: This study aims to identify the frequency and clinical-pathological correlation of glomerular diseases diagnosed at the HC-UFPR over the period of 5 years. Methods: 131 biopsies were performed between January 1, 2008 and December 31, 2012 and were analysed by light and immunofluorescence microscopy. Histopathological slides were reviewed by a pathologist. Clinical and laboratory data and the immunofluorescence microscopy results were extracted from medical records. The findings were tabulated and analysed. Results: 128 of 131 cases were reanalysed. 46.5% were obtained from men. Patients' age averaged 43 years for men and 38 for women. In 99 cases, the indication of biopsy was identified; 49.5% cases presented nephrotic syndrome, 17.17%, acute renal failure and 15.15%, chronic renal failure; 8.08%, nephritic syndrome; 6.06%, isolated proteinuria and 4.04% isolated hematuria. In 61.21% an underlying disease related to the glomerulopathy could be identified; 33.62% corresponded to primary disease and in 5.17% of cases the nature of the glomerulopathy could not be determined. Among secondary glomerulopathies, the most frequent was Lupus Nephritis (49.29%), and among the primary, Focal Segmental Glomerulosclerosis (30.77%) and Membranous Nephropathy (25.64%). Conclusion: The average patient with glomerulopathy in this service is an adult with nephrotic syndrome. Unlike other reports, secondary glomerulopathies were predominant. These findings may reflect the tertiary characteristic of the assistance at HC-UFPR.
DOI: 10.6084/m9.figshare.19963619
title: Podocyturia in Fabry disease

description: Abstract Introduction: Fabry disease is a lysosomal storage disorder due to abnormalities in the GLA gene (Xq22). Such changes result in the reduction/absence of activity of the lysosome enzyme α-GAL, whose function is to metabolize globotriaosylceramide (Gb3). Renal disease is a major clinical outcome of the accumulation of Gb3. Podocyte injury is thought to be a major contributor to the progressive loss of the renal function and may be found altered even before the onset of microalbuminuria. Objective: The aim of this study was to quantify the urinary excretion of podocytes in Fabry disease patients (V269M, n = 14) and healthy controls (n = 40), and to correlate podocyturia with the variables gender, age, time of therapy and albumin: creatinine ratio (ACR). Methods: Urinary podocytes were stained using immunofluorescence to podocalyxin and DAPi. The number of podocalyxin-positive cells was quantified and the average number was taken (normal range 0-0.6 podocytes/mL). Results: The average number of podocytes in the urine of Fabry disease patients was significantly higher than in healthy controls (p &lt; 0.0001). We observed a positive correlation between podocyturia and ACR (p = 0.004; (r2 = 0.6417). We found no correlation between podocyturia and gender, age or duration of therapy. Conclusion: Podocyturia is an important parameter in the assessment of renal disease in general, and it may serve as an additional early tool for monitoring Fabry disease nephropathy even before changes in ACR are seen. This may prove to be a useful tool to assess disease progression in patients expected to have a more aggressive phenotype.
DOI: 10.6084/m9.figshare.19963619.v1
title: Podocyturia in Fabry disease

description: Abstract Introduction: Fabry disease is a lysosomal storage disorder due to abnormalities in the GLA gene (Xq22). Such changes result in the reduction/absence of activity of the lysosome enzyme α-GAL, whose function is to metabolize globotriaosylceramide (Gb3). Renal disease is a major clinical outcome of the accumulation of Gb3. Podocyte injury is thought to be a major contributor to the progressive loss of the renal function and may be found altered even before the onset of microalbuminuria. Objective: The aim of this study was to quantify the urinary excretion of podocytes in Fabry disease patients (V269M, n = 14) and healthy controls (n = 40), and to correlate podocyturia with the variables gender, age, time of therapy and albumin: creatinine ratio (ACR). Methods: Urinary podocytes were stained using immunofluorescence to podocalyxin and DAPi. The number of podocalyxin-positive cells was quantified and the average number was taken (normal range 0-0.6 podocytes/mL). Results: The average number of podocytes in the urine of Fabry disease patients was significantly higher than in healthy controls (p &lt; 0.0001). We observed a positive correlation between podocyturia and ACR (p = 0.004; (r2 = 0.6417). We found no correlation between podocyturia and gender, age or duration of therapy. Conclusion: Podocyturia is an important parameter in the assessment of renal disease in general, and it may serve as an additional early tool for monitoring Fabry disease nephropathy even before changes in ACR are seen. This may prove to be a useful tool to assess disease progression in patients expected to have a more aggressive phenotype.
DOI: 10.6084/m9.figshare.19963643
title: Hypoalbuminemia seems to be associated with a higher rate of hospitalization in hemodialysis patients

description: Abstract Introduction: Anemia, inflammation and hypoalbuminemia are frequent disorders among patients underwent hemodialysis. There are few national data, particularly from Northeast region where anemia and malnourished were common findings, analyzing the association between these conditions and clinical outcomes. Objective: The aim of this study was to evaluate the impact of the presence of anemia, inflammation and hypoalbuminemia on clinical outcomes (death and hospitalization) of hemodialysis patients. Methods: In this prospective observational study 221 adult patients were evaluated, considering the presence of anemia (hemoglobin ≤ 10 g/dL), inflammation (C-reactive protein (CRP) ≥ 0,5 mg/dL) and hypoalbuminemia (albumin &lt; 3,8 g/ dL) at baseline. Clinical outcomes were recorded over 13 months. Results: The occurrence of hospitalization and death did not differ between the groups with and without anemia or inflammation. Patients with hypoalbuminemia had more hospitalizations, and the presence of hypoalbuminemia was associated with shorter hospitalization event-free time (p = 0.008). There was a trend of shorter hospitalization event-free time among patients with increased PCR (p = 0.08). There was no correlation between albumin and CRP levels. The presence of anemia, inflammation and hypoalbuminemia were not associated with lower survival. Adjusting for confounders, hypoalbuminemia was a predictor of hospitalization in hemodialyzed patients. Conclusion: The presence of hypoalbuminemia, but not anemia or inflammation, was able to predict hospitalization in hemodialysis patients.
DOI: 10.6084/m9.figshare.19963643.v1
title: Hypoalbuminemia seems to be associated with a higher rate of hospitalization in hemodialysis patients

description: Abstract Introduction: Anemia, inflammation and hypoalbuminemia are frequent disorders among patients underwent hemodialysis. There are few national data, particularly from Northeast region where anemia and malnourished were common findings, analyzing the association between these conditions and clinical outcomes. Objective: The aim of this study was to evaluate the impact of the presence of anemia, inflammation and hypoalbuminemia on clinical outcomes (death and hospitalization) of hemodialysis patients. Methods: In this prospective observational study 221 adult patients were evaluated, considering the presence of anemia (hemoglobin ≤ 10 g/dL), inflammation (C-reactive protein (CRP) ≥ 0,5 mg/dL) and hypoalbuminemia (albumin &lt; 3,8 g/ dL) at baseline. Clinical outcomes were recorded over 13 months. Results: The occurrence of hospitalization and death did not differ between the groups with and without anemia or inflammation. Patients with hypoalbuminemia had more hospitalizations, and the presence of hypoalbuminemia was associated with shorter hospitalization event-free time (p = 0.008). There was a trend of shorter hospitalization event-free time among patients with increased PCR (p = 0.08). There was no correlation between albumin and CRP levels. The presence of anemia, inflammation and hypoalbuminemia were not associated with lower survival. Adjusting for confounders, hypoalbuminemia was a predictor of hospitalization in hemodialyzed patients. Conclusion: The presence of hypoalbuminemia, but not anemia or inflammation, was able to predict hospitalization in hemodialysis patients.
DOI: 10.6084/m9.figshare.19963638
title: Brazilian Chronic Dialysis Census 2014

description: Abstract Introduction: National chronic dialysis data have had impact in the treatment planning. Objective: To report data of the annual survey of the Brazilian Society of Nephrology about chronic kidney disease patients on dialysis in July 2014. Methods: A survey based on data of dialysis units from the whole country. The data collection was performed by using a questionnaire filled out on-line by the dialysis units. Results: Three hundred twelve (44%) of the dialysis units in the country answered the questionnaire. In July 2014, the total estimated number of patients on dialysis was 112,004. The estimated prevalence and incidence rates of chronic maintenance dialysis were 552 (range: 364 in the North region and 672 in the Southeast) and 180 patients per million population (pmp), respectively. The annual incidence rate of patients with diabetic nephropathy was 77 pmp. The annual gross mortality rate was 19%. For prevalent patients, 91% were on hemodialysis and 9% on peritoneal dialysis, 32,499 (29%) were on a waiting list of renal transplant, 37% were overweight/obese, 29% were diabetics, 16% had PTH levels &gt; 600 pg/ml and 26% hemoglobin &lt; 10 g/dl. A venous catheter was the vascular access for 17% of the hemodialysis patients. Conclusion: During 2011-2014 the prevalence and incidence rates of patients on dialysis tended to increase, while the gross mortality rate remained stable. In 2014, diabetes was the primary renal disease in 42% of the new dialysis patients.
DOI: 10.6084/m9.figshare.19963638.v1
title: Brazilian Chronic Dialysis Census 2014

description: Abstract Introduction: National chronic dialysis data have had impact in the treatment planning. Objective: To report data of the annual survey of the Brazilian Society of Nephrology about chronic kidney disease patients on dialysis in July 2014. Methods: A survey based on data of dialysis units from the whole country. The data collection was performed by using a questionnaire filled out on-line by the dialysis units. Results: Three hundred twelve (44%) of the dialysis units in the country answered the questionnaire. In July 2014, the total estimated number of patients on dialysis was 112,004. The estimated prevalence and incidence rates of chronic maintenance dialysis were 552 (range: 364 in the North region and 672 in the Southeast) and 180 patients per million population (pmp), respectively. The annual incidence rate of patients with diabetic nephropathy was 77 pmp. The annual gross mortality rate was 19%. For prevalent patients, 91% were on hemodialysis and 9% on peritoneal dialysis, 32,499 (29%) were on a waiting list of renal transplant, 37% were overweight/obese, 29% were diabetics, 16% had PTH levels &gt; 600 pg/ml and 26% hemoglobin &lt; 10 g/dl. A venous catheter was the vascular access for 17% of the hemodialysis patients. Conclusion: During 2011-2014 the prevalence and incidence rates of patients on dialysis tended to increase, while the gross mortality rate remained stable. In 2014, diabetes was the primary renal disease in 42% of the new dialysis patients.