cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14455/tsb.res.2019.44
title: Molecular Epidemiology of Carbapenem Resistance Among Multidrug-resistant Pseudomonas aeruginosa isolated Clinically in Tertiaries Care Centers, Southern Thailand

description: Pseudomonas aeruginosa is one of potent opportunistic nosocomial human pathogen that shows resistance to a multiple class of antibiotics with carbapenems being the most potent inhibitor of P. aeruginosa. This study aimed to investigate the prevalence of metallo-?-lactamases (MBLs) genes and antimicrobial susceptibilities of P. aeruginosa isolated in two tertiaries care hospitals of Songkhla Province. A total number of 209 P. aeruginosa isolates were collected between August 2015 and December 2016 from various clinical samples in two tertiaries care hospitals of Songkhla Province. Antibiotic susceptibility was determined using the disk diffusion method. MBLs genes were detected by multiplex-PCR and blasted. Out of 209 P. aeruginosa, 198 ( 94. 7%) was carbapenem- resistant isolates. The susceptibility rate of P. aeruginosa toward polymyxin B and Amikacin was 100% and 48.3% respectively. The highest of Pseudomonas infections were found in sputum 111 (53.1%), urine 37 (17.7%) and secretions 17 (8.1%) of P. aeruginosa isolates. It was also found that the rate of multidrug resistance P. aeruginosa infection was 36.4% in the medical ward, 20.1% in ICU and 12.9% in surgical wards. The most dominant MBLs genes among P. aeruginosa isolates was blaDIM (53.6%), while the prevalence of blaVIM, blaIMP and blaAIM were 29.2%, 12.4% and 9.6% respectively, whereas blaoxa48 was detected in only 9.6% of carbapenemase-positive isolates. The results showed that the prevalence of multidrug resistance was high and the presence of more than one blaMBLs gene in P. aeruginosa isolates to emphasize the challenge to therapeutic treating emerging.
DOI: 10.14455/tsb.res.2019.45
title: Phenolic and Flavonoid Contents Isolated from the Red Seaweed, Acanthophora spicifera

description: Nowadays, seaweeds have been considered as sources of bioactive compounds used for antioxidant and anti-pathogen in medical experiments and cosmetic ingredients including used as elicitor for induced resistance in agricultural sciences. Acanthophora spicifera is a non-valuable red seaweed that can be found in Andaman sea and gulf of Thailand. In this study, we have used this red seaweed to search for its bioactive compounds that could be served as alternative sources. Two methods were compared between extracted with hot water and methanol. Using hot water, we obtained high content of phenolic
description: in contrast, using methanol gave high amount of flavonoid. The phenolic content using hot water was 3.42 mg gallic acid equivalent/g dry weight and flavonoid content extracted with methanol was 2.93 mg rutin equivalent/g DW. We measured in vitro antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) methods. For DPPH, the IC50 of DPPH scavenging activity using hot water and methanol were 7.33 mg and 25.32 mg, respectively. For FRAP, the hot water extract had reducing activity higher than the methanol extract which were 8.08 and 4.66 mg ascorbic acid equivalent/g DW, respectively. In conclusion, the hot water extract had antioxidant activity higher than the methanol extract. These results provided the evident of using low cost extraction method but obtained higher amount of active compounds
description: consequently, could up value of this non-valuable seaweed. The ABTS Scavenging of (2,2-azinobis-(3ethylbenzothiazoline-6-sulfonic acid)) activity, anti-tyrosinase and antibacterial activities will be further conducted before applying these active compounds on food and cosmetic industries.
DOI: 10.14455/tsb.res.2019.46
title: Physicochemical , Microbial, and Sensory Properties of Yoghurt Supplement with Inulin Rich Carbohydrates Extract from Jerusalem Artichoke Tubers during Storage

description: The purpose of this study was to evaluate the effect of inulin rich carbohydrates extract from Jerusalem artichoke tubers on physicochemical, textural, and microbiological properties of the yoghurt during storage. Five formulations of yogurt were prepared with the addition of 0, 1, 3, 5, 7 or 10% of inulin rich carbohydrates extract from Jerusalem artichoke tubers. During storage at 4?C, the pH of yoghurt treatments supplement with 3, 5 and 7% inulin extract from Jerusalem artichoke tubers and the control (0% adding) was rapidly decreased during 14 days storage (from 4.45 to 4.36). The viscosity was slightly increased and reached the highest value of 3080 Cps. in the yoghurt supplement with 7 and 10% inulin extract from Jerusalem artichoke tubers. Both L. delbrueckii subsp. bulgaricus and S. thermophilus were not able to ferment inulin extract from Jerusalem artichoke tubers under the conditions study due to no significant survival counts of the yoghurt strains neither acidification between the yoghurt treatments supplement with inulin extract from Jerusalem artichoke tubers and the control was observed. Inulin rich carbohydrates extract from Jerusalem artichoke tubers causes alterations in sensory perception. More concentrations of inulin extract from Jerusalem artichoke tubers adding resulted to more slightly decrease in overall acceptance compare to the control yoghurt. However, yoghurt adding with 3% inulin extract from Jerusalem artichoke tubers was improved some physical properties (flavor and texture) and reach the highest score (8.10) in overall acceptance compare to control yoghurt (8.00).
DOI: 10.14455/tsb.res.2019.47
title: Process Optimization for Influenza NA-VLP Vaccine Development using Baculovirus/Insect Cell System

description: Recombinant protein based virus- like particles ( VLPs) vaccine produced in insect cell using baculovirus expression vector was one of the methods developed to overcome the limitations of influenza vaccine eggbased production. This study focused on bioprocess development of influenza NA-VLP vaccine from the baculovirus/insect cells system. The NA-VLP production and purification processes were optimized. Infection of recombinant NA baculovirus at MOI 1 into 1x106 cells/ml insect cells in active growing phase for 4 days resulted in the highest NA-VLP level. The NA-VLP could be mostly purified by two conventional methods i.e. ultrafiltration and size exclusion chromatography. Firstly, NA- VLP was concentrated and purified from a major contaminant protein, albumin, by the cross flow ultrafiltration using 500 kDa membrane. More than 97% of albumin removal was achieved in this process. The largest NA-VLP could be obtained after size exclusion chromatography. Albumin was also further removed from this step. It was found that recombinant NA baculovirus could not be efficiently removed by these two steps purification process. One more simple purification by anion exchange ( AEX) chromatography using flow- through mode is recommended.
DOI: 10.14455/tsb.res.2019.48
title: Purification and characterization of Pleurotus sajor-caju (Fr.) Sing gluco-oligosaccharide (Ps-GOS) obtained from Hevea ?1,3-glucanases digestion of insoluble ?-Glucans

description: The objective of this study was to purify and characterize of Pleurotus sajor-caju (Fr.) Sing gluco-oligosaccharide (Ps-GOS) obtained from the digestion of its insoluble ?- glucan with ?- 1, 3- glucanase derived from concentrated Hevea latex serum. A short-chains and water soluble Ps-GOS was characterized, with LC-MS and IR, and confirmed to be a mixture of ?-1, 3/1, 6-glucan. Further purification of Ps-GOS with anion -exchange (DEAEcellulose) and size-exclusion (Bio-Gel P10) chromatography were applied and obtain a various size of purified gluco-oligosaccharide with a molecular weight range from 1.5 to 20 kDa. In addition, our preliminary study was demonstrated that Ps-GOS could inhibit tumor growth and metastasis of human tumor, CaSki and B16F1 cell, xenografts in immunodeficient mice. Moreover, the immunohistochemistry study indicated that the level of inflammatory activity after mice were treated with Ps-GOS was decreased. Nevertheless, to further understand the anti-inflammatory mechanism, we will investigate its anti- inflammatory activities of purified Ps- GOS in lipopolysaccharide-induced RAW264.7 macrophage.
DOI: 10.14455/tsb.res.2019.49
title: Roles of Histone Deacetylases in Orbital Fibroblasts from Graves' Ophthalmopathy Patients

description: Graves' ophthalmopathy (GO) is an autoimmune thyroid eye disease which mainly causes eyelid retraction and proptosis. Orbital fibroblasts play an important role in GO pathogenesis by increasing cells proliferation, cytokines and hyaluronan production and adipogenesis. Nevertheless, current GO treatments remain limited and novel treatments are needed. Histone deacetylases (HDACs) are enzymes that remove acetyl group from histone tails which plays a role in the regulation of gene expression. HDACs are involved in the pathogenesis of various diseases, including cancer and autoimmune diseases. Therefore, treatment with HDAC inhibitors ( HDACis) was conducted. Currently, the roles of HDACs in orbital fibroblasts from GO remain unidentified. Hence, the aim of this study was to investigate the roles of HDACs in PDGF-BB-induced orbital fibroblast activation using isoformselective HDACis. The results showed that prophylactic effect of HDAC3i (RGFP966), HDAC4i (Tasquinimod) and HDAC4/5i (LMK235) significantly decreased PDGF- BB- induced cell proliferation. In addition, HDAC3i, HDAC4i and HDAC4/5i significantly reduced PDGF-BB-induced hyaluronan production. Unexpectedly, prophylactic effect of HDAC1i ( Quisinostat) , HDAC3i and HDAC4i significantly enhanced PDGF- BB- induced IL- 6 production, while IL-8 production was not affected by HDACis. The level of HDAC3 and HDAC4 expressions increased in PDGF-BB-stimulated orbital fibroblasts. Thus, the data from this study suggested that aberrant HDAC4 levels are involved in PDGF-BB-induced orbital fibroblasts activation.
DOI: 10.14455/tsb.res.2019.50
title: Screening and Isolation of Stevioside and Rebaudioside A Degrading Bacteria from Forest Soil

description: Stevioside and rebaudioside A are the two major steviol glycosides found in Stevia rebaudiana leaves. These glycosides have been widely utilized as natural sugar substitutes in food and beverage industries. Unfortunately, the steviol glycosides quantification are still relied on laborious techniques and expensive analytical instruments, such as mass spectrometry and highperformance liquid chromatography. These analytical techniques are not only time-consuming but also requiring skilled technicians. Previously, an established enzymatic method was developed as a more convenient and inexpensive alternative assay to evaluate stevioside quantity in plant specimen. This study was aimed to screen and identify bacteria with enzymatic capabilities to degrade stevioside and rebaudioside A from soil. The soil samples were collected from the deciduous forests in Nan province, Thailand. A total of 108 bacterial strains were isolated, using minimal salt medium supplemented with stevia leave extract as a sole carbon source. The stevioside and rebaudioside A degradation abilities of each bacterial isolate were investigated using thin-layer chromatography (TLC). Stevioside degrading activity was observed in 3 bacterial isolates. One of them appears to be able to degrade both substrates. The identification based on the sequence of 16S rRNA gene suggested that the bacterial isolates belong to genus Burkholderia, Brevibacillus and Bacillus respectively. Whole genome sequencing of the bacterial isolate capable of degrading both stevioside and rebaudioside A was further performed. The candidate genes responsible for the glycoside degrading activity were preliminarily annotated. While there were a few previous reports of steviol glycosides degrading bacteria, the enzymatic activity reported here appeared to be unique. The glycoside degrading enzymatic activity revealed in this work could be fruitful for broadening the applicability of the enzymatic approach to determine the content of both stevioside and rebaudioside A in Stevia samples.
DOI: 10.14455/tsb.res.2019.51
title: Screening of potential probiotic Bacillus for aquaculture industry

description: The objective of this study was to isolate probiotic Bacillus from intestinal contents of fishes and shrimps. 154 Bacillus- like bacteria were isolated and studied further based on probiotic characteristics. A total of 5 isolated bacteria was selected as they were not potential pathogenic bacteria. Besides, they exhibited antimicrobial activity against specific pathogens such as Vibrio alginolyticus, Vibrio parahaemolyticus, Pseudomonas aeroginosa and Bacillus cereus and tolerance to some antimicrobial substances. These isolates were identified by using the API 50 CHB identi?cation kit and confirmed by using the 16S rRNA sequence. Results showed that the potential probiotic bacteria were Bacillus safensis, Bacillus subtilis and Brevibacillus agri.
DOI: 10.14455/tsb.res.2019.52
title: Study of Bitter Leaf Beer Formulations and Bitter Hops Substitution

description: The objective of this research was to study the possibility of making good quality craft beer by using Vernonia Amygdalina to substitute bitter hops. According to its bitter taste, it was fondly called as Bitter leaf. Anti-nutritional phytochemicals within the plants are responsible for the bitter taste. Bioactive compounds such as alkaloids, saponins, terpenes, flavonoids, phenolic acids, steroids, coumarins, and etc., are responsible for its ethnobotanical uses. From previous researches, Bitter Leaf was reported as a well-known as medical plant with several uses. Therefore, in this study, Bitter Leaf was used as one of the supplement ingredients in our brewing. In this research, final Bitter Leaf beer formulation has been chosen from consumer preference done by sensory evaluation. There are many differences varieties of bitter herbs used to substitute bitter hops. Since the anti-nutritional properties and phytochemical compounds which are functioning as anti-microbial and anti-bacterial agents. Both are the most important properties that could be use instead of bitter hops. However, one last important property that Bitter Leaf could not be substituting bitter hops completely is the foam stabilization. In conclusion, Bitter Leaf could be substituted bitter hops since its characteristic and properties are similar to bitter hops. By the way of contrast, the flavor of beer might be different from commercial beers which brewed by using bitter hops. To guarantee the beer's shelf life, a modern preservation is needed.
DOI: 10.14455/tsb.res.2019.53
title: Synergistic effect of water extract from combined edible mushrooms

description: The synergistic effect of the water extract from Gold cordyceps (Cordyceps militaris), Shiitake (Lentinus edodes) and Lingzhi (Garnoderma lucidum) were studied. The results showed that the mixture of C. militaris and G. lucidum gave highest yield. However, polysaccharide content was no statistically significant difference in all extracts. Then, the extracts and mixture of extracts were test for antimicrobial activity, antioxidant activity and ?-amylase inhibition. The result revealed that none of extracts showed antimicrobial activity. Interestingly, the mixture of C. militaris and G. lucidum extracts showed synergistic of antioxidant activity. The lowest IC50 of scavenging activity was 0.12?0.03 mg/ml. The mixture of three mushrooms extract exhibited ?-amylase inhibition by 69.72?1.20%.
DOI: 10.14455/tsb.res.2019.54
title: The alternative source of high GABA content derived from germinated black bean

description: Gamma-aminobutyric acid (GABA) is a kind of biological component which is also considered as one of the inhibitory neurotransmitter in the nervous system. GABA source is generally found in bean seeds, but its production was very difficult to have the biological effect due to the existing of low GABA concentration. To enrich the GABA content in the beans, seed germination process was conducted. Therefore, this study was aimed to investigate the effect of germination times for GABA production in black bean seeds. For this reason, the black bean seeds were germinated and observed at different germination times of 0 h (after soaking immediately), 6 h, 12 h, 24 h, and 36 h, respectively. The result revealed that the highest of GABA content in germinated black beans were found at 4.78 mg/g (24 h after germination) which was 7.83 times higher than the nongerminated black beans (0.61 mg/g). However, the concentration of GABA was decreased probably 2.53 times after 36 h (1.89 mg/g) from the maximum content, likely due to the plant growth metabolism. In overall, this current study confirmed that the germination process could be used as an alternative way to provide high GABA content for the future bean products, especially black bean milk and sprouts.
DOI: 10.14455/tsb.res.2019.55
title: The Effect of Histone Methyltransferase In Orbital Fibroblast From Graves' Ophthalmopathy

description: Graves' ophthalmopathy (GO) is an extra-thyroidal complication of Graves' disease (GD). The clinical presentations of GO are upper eyelid retraction, erythema of periorbital tissue, conjunctivitis, proptosis and fibrosis. Some patients have severe symptoms such as optic neuritis leading to sightthreatening symptoms such as exposure keratitis and optic nerve compression or dysthyroid optic neuropathy. Platelet-derived growth factor-BB (PDGF-BB) is an important growth factor in the pathogenesis of GO. As orbital fibroblasts in orbital tissue express platelet-derived growth factor receptors (PDGF-R) on the surface, PDGF-BB stimulates orbital fibroblasts activities such as cytokine and hyaluronan production, proliferation and adipogenesis. Presently, active GO is treated with high-dose systemic glucocorticoids (GCs) as first line treatment which also carries several serious side effects, including hyperglycemia, fulminant hepatitis and a generalized state of immunosuppression. Therefore, novel therapeutic strategies with fewer complications are needed for GO. Epigenetic modifications control gene expression. One of the mechanisms is histone modification at the N-terminal amino acid. Histone methylation is the process of transferring methyl groups to lysine or arginine residues on the histone tail by a group of enzymes called histone methyltransferases (HMTs). In this study, we investigated the role of histone lysine methyltransferases ( KHMTs) and histone arginine methyltransferase ( PRMT5) in PDGF- BB- induced orbital fibroblasts activation by using specific inhibitors. Inhibition of KHMT (with DZNeP, BIX01294 or Pinometostat) as well as PRMT5 (with GSK591) inhibited PDGF-BBinduced hyaluronan production by orbital fibroblasts, both in a prophylactic and more treatment related experimental set-up. Inhibition of KHMT with DZNeP also prevented PDGF-BB-induced production of IL-6 and IL- 8 by orbital fibroblasts, while BIX01294 DNNeP, pinometostat and GSK591 also inhibited PDGF-BB-induced orbital fibroblasts proliferation, again both in a prophylactic and more treatment related experimental set-up. These data from inhibitors screening suggested that the KHMT enzymes (G9a, EZH2 and DOT1L) and PRMT5 induce orbital fibroblasts proliferation, cytokine and hyaluronan production.
DOI: 10.14455/tsb.res.2019.56
title: The Partial Purification of Water-Soluble Cellulose from Banana Peel Extraction

description: Banana peel is one of the lignocellulosic wastes from food processing and often used as a raw material for cellulose extraction. To obtain watersoluble cellulose, the extracted cellulose was hydrolysed by cellulase. The purification was needed since the process of hydrolysis provided mixed products of glucose, cello-oligosaccharides, as well as soluble cellulose. Thus, the aim of this study was to partially purify the soluble cellulose from the enzymatic hydrolysate. Initially, cellulose fiber was extracted from banana peel by alkaline and bleaching treatment. Cellulose extracted was then hydrolyzed by commercial cellulase. The hydrolysate was purified by using ethanol precipitation. The purification was investigated by thin layer chromatography and glucose kit assay. The results revealed that the ethanol could precipitate the hydrolysate into 2 phases, the supernatant and the ethanol insoluble pellet. The thin layer chromatography depicted that glucose, cellobiose, cellotriose, and cellotetraose were detected in the supernatant. The ethanol insoluble pellet was water-soluble cellulose. Moreover, the glucose kit assay could not detect any glucose in the pellet. Therefore, this convenient purification could be effectively used for water-soluble cellulose production. This soluble fiber would be further studied as a food additive.
DOI: 10.14455/tsb.res.2019.57
title: Upscaling of RS87 endospore production process - from flask to semi-industrial scale cultivation

description: Utilization of PGPR (plant growth promoting rhizobacteria) has been documented to enhance crop yield and reduce the usage of agrochemicals. Bacillus cereus strain RS87 has been proven for its PGPR properties as well as an ability for 50% chemical fertilizer substitution in several crops (i.e. rice, pepper, cucumber and tomato). Nonetheless, the conventional process for RS87 endospore production using 0.5xTSB (tryptic soy broth) in a flask-scale cultivation was not a cost-effectiveness and resulted in a low spore yield (2x107 spores/mL after 4 days). In order to make the process compatible for a large-scale production, an effective low-cost cultivation medium is inevitable. Beside, the cultivation conditions in a bioreactor-scale must be established. An optimized cultivation medium previously developed for RS87 by Jenjitwanich et al. (2018) or omCCY was proven to yield good growth and endospore production at a flask-scale. In this study, omCCY was employed in an upscaling cultivation using a 5 L-bioreactor. Effects of %dissolved oxygen (%DO) and mode of cultivation (batch vs fed-batch) on growth and endospore production of RS87 were investigated. The 24 h-batch cultivation with low %DO (10-20%) yielded a comparable maximum spore count (3.4x108 spores/mL after 24 h) to that of flask-scale cultivation. Maintaining of %DO at a moderate level (40-50%) allowed us to obtain the maximum spore count of 2.5x108 spores/mL within only 12 h. Finally, the fed-batch cultivation with pulse additions of nutrients (glycerol + yeast extract) and divalent ions further improved the endospore yield to 5.2x108 spores/mL (at 12 h).
DOI: 10.17632/6txxrscxv7.1
title: Data for: Construction and Quantification of a Fire Probabilistic Safety Assessment Model Using Support System Initiating Event Fault Trees

description: Resulting Figures and Tables for FT Modeling of the Fire-induced IEs
DOI: 10.25919/5dc4eca55daf9
title: Parkes observations for project P574 semester 2019OCTS_02

description: We request time to observe 260 pulsars on a regular basis to provide the accurate ephemerides necessary for the detection and characterisation of gamma-ray pulsars with the Fermi satellite. The primary science goals are to increase the number of known gamma-ray pulsars (both radio loud and radio quiet), to determine accurate pulse profiles, and to characterise their high energy (phase-resolved) spectra. In the radio, the observations will also allow us to investigate: neutron stars and pulsar emission via detecting glitches and determining pulse timing parameters; and the ISM via measuring and monitoring dispersion measures, Faraday rotation measures, and scintillation. To date, we are (co-)authors on 75 papers arising from the collaboration and P574 data. The data have contributed to the PhD theses of students from Bordeaux, Manchester, Oxford, Stanford, and Swinburne.
DOI: 10.26300/yxjs-m578
title: feronia_Athaliana_x_WT_Alyrata_Rep_01