cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21835402.v1
title: Major depressive episode in hospital workers during the Covid-19 pandemic in Brazil

description: ABSTRACT OBJECTIVES To estimate prevalence and factors associated with major depressive episode (MDE), emphasizing occupational aspects, in workers of a public teaching hospital that is a reference for Covid-19 treatment. METHODS A cross-sectional study was carried out between October and December 2020, after the first peak of the pandemic, interviewing 1,155 workers. The prevalence of MDE was estimated using the Patient Health Questionnaire (PHQ-9) algorithm. Multivariate hierarchical analysis was conducted using Poisson regression to assess associated factors. RESULTS MDE prevalence was 15.3% (95%CI: 13.3–17.5) and was higher among young, white and female workers, those with a family history of depression, resident professionals, nursing professionals, workers who were exposed to three or more situations of moral dilemma, and those who had to put off a physiological need until later. Having a risk factor for Covid-19, being a smoker and being physically inactive were also positively associated with MDE. CONCLUSIONS The study points to the considerable prevalence of MDE among tertiary health care workers; reviewing work processes is essential to reduce occupational stress and minimize the effects of the pandemic on mental health, preventing those problems from becoming chronic.
DOI: 10.6084/m9.figshare.21835404
title: Soil quality indicators for Urochloa brizantha fertilized with wood ash

description: ABSTRACT The objective was to evaluate the effect of wood ash dose and application management on soil quality attributes in a Cerrado area cultivated with Urochloa brizantha. The experiment was carried out in a randomized block design in a 5 × 2 strip-plot scheme and four repetitions. The ash doses were 0, 8, 16, 24, and 32 t ha-1, and the application management considered wood ash that is incorporated and not incorporated into the soil. The P, K, and pH values of the soil increased with an increase in the doses of wood ash. The soil bulk density decreased by 9.66% with the wood ash doses of 24 and 32 t ha-1 incorporated into the soil. Owing to an increase in the soil pH, a wood ash dose of greater than 16 t ha-1 decreased the enzymatic activity. A wood ash dose of 24 t ha-1 resulted in better conditions for the soil, improving its quality and contributing to the balance and dynamics between the chemical, physical, and biological attributes of the soil, which led to the reduced density and greater availability of P in the soil.
DOI: 10.6084/m9.figshare.21835404.v1
title: Soil quality indicators for Urochloa brizantha fertilized with wood ash

description: ABSTRACT The objective was to evaluate the effect of wood ash dose and application management on soil quality attributes in a Cerrado area cultivated with Urochloa brizantha. The experiment was carried out in a randomized block design in a 5 × 2 strip-plot scheme and four repetitions. The ash doses were 0, 8, 16, 24, and 32 t ha-1, and the application management considered wood ash that is incorporated and not incorporated into the soil. The P, K, and pH values of the soil increased with an increase in the doses of wood ash. The soil bulk density decreased by 9.66% with the wood ash doses of 24 and 32 t ha-1 incorporated into the soil. Owing to an increase in the soil pH, a wood ash dose of greater than 16 t ha-1 decreased the enzymatic activity. A wood ash dose of 24 t ha-1 resulted in better conditions for the soil, improving its quality and contributing to the balance and dynamics between the chemical, physical, and biological attributes of the soil, which led to the reduced density and greater availability of P in the soil.
DOI: 10.6084/m9.figshare.21835405
title: Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens

description: Abstract Based on a holistic concept of polymicrobial etiology, we have hypothesized that putative and candidate periodontal pathogens are more frequently detected in consortia than alone in advanced forms of periodontal diseases (PD). Objective To correlate specific consortia of periodontal pathogens with clinical periodontal status and severity of periodontitis. Methodology Subgingival biofilm was obtained from individuals with periodontal health (113, PH), gingivitis (91, G), and periodontitis (209, P). Genomic DNA was purified and the species Aggregatibacter actinomycetemcomitans (Aa), Aa JP2-like strain, Porphyromonas gingivalis (Pg), Dialister pneumosintes (Dp), and Filifactor alocis (Fa) were detected by PCR. Configural frequency and logistic regression analyses were performed to correlate microbial consortia and PD. Results Aa + Pg in the presence of Dp (phi=0.240; χ2=11.9, p&lt;0.01), as well as Aa JP2 + Dp + Fa (phi=0.186, χ2=4.6, p&lt;0.05) were significantly more associated in advanced stages of P. The consortium Aa + Fa + Dp was strongly associated with deep pocketing and inflammation (p&lt;0.001). The best predictors of disease severity (80% accuracy) included older age (OR 1.11 [95% CI 1.07 – 1.15], p&lt;0.001), Black/African-American ancestry (OR 1.89 [95% CI 1.19 – 2.99], p=0.007), and high frequency of Aa + Pg + Dp (OR 3.04 [95% CI 1.49 – 6.22], p=0.002). Conclusion Specific microbial consortia of putative and novel periodontal pathogens, associated with demographic parameters, correlate with severe periodontitis, supporting the multifactorial nature of PD.
DOI: 10.6084/m9.figshare.21835405.v1
title: Periodontal disease severity is associated to pathogenic consortia comprising putative and candidate periodontal pathogens

description: Abstract Based on a holistic concept of polymicrobial etiology, we have hypothesized that putative and candidate periodontal pathogens are more frequently detected in consortia than alone in advanced forms of periodontal diseases (PD). Objective To correlate specific consortia of periodontal pathogens with clinical periodontal status and severity of periodontitis. Methodology Subgingival biofilm was obtained from individuals with periodontal health (113, PH), gingivitis (91, G), and periodontitis (209, P). Genomic DNA was purified and the species Aggregatibacter actinomycetemcomitans (Aa), Aa JP2-like strain, Porphyromonas gingivalis (Pg), Dialister pneumosintes (Dp), and Filifactor alocis (Fa) were detected by PCR. Configural frequency and logistic regression analyses were performed to correlate microbial consortia and PD. Results Aa + Pg in the presence of Dp (phi=0.240; χ2=11.9, p&lt;0.01), as well as Aa JP2 + Dp + Fa (phi=0.186, χ2=4.6, p&lt;0.05) were significantly more associated in advanced stages of P. The consortium Aa + Fa + Dp was strongly associated with deep pocketing and inflammation (p&lt;0.001). The best predictors of disease severity (80% accuracy) included older age (OR 1.11 [95% CI 1.07 – 1.15], p&lt;0.001), Black/African-American ancestry (OR 1.89 [95% CI 1.19 – 2.99], p=0.007), and high frequency of Aa + Pg + Dp (OR 3.04 [95% CI 1.49 – 6.22], p=0.002). Conclusion Specific microbial consortia of putative and novel periodontal pathogens, associated with demographic parameters, correlate with severe periodontitis, supporting the multifactorial nature of PD.
DOI: 10.6084/m9.figshare.21835407
title: Infrared thermography as a technique for detecting subclinical bovine mastitis

description: ABSTRACT Infrared thermography is a non-invasive diagnostic tool for early detection of subclinical mastitis in pasture-raised dairy cows. The study was conducted on eight dairy farms, in Sena Madureira, Acre, in the Western Amazon, during the rainy season. One hundred and thirteen lactating crossbred cows were monitored, with measurements performed once a day before milking, from 02:00 to 06:00 in the morning. The California Mastitis Test (CMT) was subsequently applied. Data processing was performed in the R programming language and evaluated by linear models. Our results show that cow udder surface temperature by infrared thermography showed significant differences (p&lt;0.05) between the months of the year. Cow udder temperatures of thermographic images were significantly different (p&lt;0.05) from CMT results for animals with subclinical mastitis. CMT results showed that 45 cows (39.8%) in January, 52 cows (46%) in February and 57 cows (50.4%) in March had subclinical mastitis. Infrared thermography is a technique that can be used for the early detection of subclinical mastitis in dairy herds in a grazing system, as it detected an increase in the udder surface temperature of the same cows that were positive for CMT.
DOI: 10.6084/m9.figshare.21835407.v1
title: Infrared thermography as a technique for detecting subclinical bovine mastitis

description: ABSTRACT Infrared thermography is a non-invasive diagnostic tool for early detection of subclinical mastitis in pasture-raised dairy cows. The study was conducted on eight dairy farms, in Sena Madureira, Acre, in the Western Amazon, during the rainy season. One hundred and thirteen lactating crossbred cows were monitored, with measurements performed once a day before milking, from 02:00 to 06:00 in the morning. The California Mastitis Test (CMT) was subsequently applied. Data processing was performed in the R programming language and evaluated by linear models. Our results show that cow udder surface temperature by infrared thermography showed significant differences (p&lt;0.05) between the months of the year. Cow udder temperatures of thermographic images were significantly different (p&lt;0.05) from CMT results for animals with subclinical mastitis. CMT results showed that 45 cows (39.8%) in January, 52 cows (46%) in February and 57 cows (50.4%) in March had subclinical mastitis. Infrared thermography is a technique that can be used for the early detection of subclinical mastitis in dairy herds in a grazing system, as it detected an increase in the udder surface temperature of the same cows that were positive for CMT.
DOI: 10.6084/m9.figshare.21835408
title: Nicotine as a Catalyst for Chlorination Promoted by Hypochlorous Acid: Experimental and Theoretical Studies

description: There is robust evidence of the hypochlorous acid (HClO)-mediated damage in biomolecules, and nicotine boosts the chlorination potency of HClO. We present experimental and theoretical evidence of the mechanism by which nicotine catalyzes the chlorination of pyranine. The rate constants for chlorination of pyranine by HClO were measured in the presence (5.3 × 105 mol 1 L s-1) and absence of nicotine (4.2 × 103 mol-1 L s-1), revealing the catalytic effect of the alkaloid. Density functional theory (DFT) calculations, based on B3LYP-GD3(BJ)/6-311++G(3df,2p)/SMD(Water)//B3LYP-GD3(BJ)/6-311++G(2d,p)/SMD(Water) level of theory, were performed and showed a decreased activation energy for chlorine transfer and hydrogen abstraction when nicotine chloramine intermediated the reaction. The atomic polar tensor (APT) charges on the chlorine atom of HClO (+0.084) were lower than the chlorine of nicotine chloramine (+0.149), revealing the higher electrophilic character of nicotine chloramine. In conclusion, the increased electrophilic nature of HClO provoked by nicotine explains its catalytic effect.
DOI: 10.6084/m9.figshare.21835408.v1
title: Nicotine as a Catalyst for Chlorination Promoted by Hypochlorous Acid: Experimental and Theoretical Studies

description: There is robust evidence of the hypochlorous acid (HClO)-mediated damage in biomolecules, and nicotine boosts the chlorination potency of HClO. We present experimental and theoretical evidence of the mechanism by which nicotine catalyzes the chlorination of pyranine. The rate constants for chlorination of pyranine by HClO were measured in the presence (5.3 × 105 mol 1 L s-1) and absence of nicotine (4.2 × 103 mol-1 L s-1), revealing the catalytic effect of the alkaloid. Density functional theory (DFT) calculations, based on B3LYP-GD3(BJ)/6-311++G(3df,2p)/SMD(Water)//B3LYP-GD3(BJ)/6-311++G(2d,p)/SMD(Water) level of theory, were performed and showed a decreased activation energy for chlorine transfer and hydrogen abstraction when nicotine chloramine intermediated the reaction. The atomic polar tensor (APT) charges on the chlorine atom of HClO (+0.084) were lower than the chlorine of nicotine chloramine (+0.149), revealing the higher electrophilic character of nicotine chloramine. In conclusion, the increased electrophilic nature of HClO provoked by nicotine explains its catalytic effect.
DOI: 10.6084/m9.figshare.21835410
title: Ten Years Assessment of Shifting Cultivation on Land Cover and Carbon Storage in Timor Island, Indonesia

description: Abstract The practice of shifting cultivation has led to forest degradation and deforestation in the Sisimeni Sanam Forest Area, with Special Purpose (SSFAwSP). Therefore, this study aims to assess land cover and carbon storage changes of the practiced shifting cultivation in SSFAwSP over the last ten years using 2013, 2016, and 2021 Landsat imagery. A hybrid classification approach that combines the forest canopy density model and supervised classification of maximum likelihood was used to create land cover maps to detect changes in forest land cover and carbon storage. The results showed that for ten years, the extent and annual rates of deforestation, forest degradation, forest regrowth, and changes in carbon storage were 662.62 ha (4%), 319.18 ha (3%), 163.8 ha (1%), and -54.51 kilo Ton C (3%), respectively. This finding indicated that shifting cultivation contributed only 10% and 1% of total deforestation and forest degradation, respectively.
DOI: 10.6084/m9.figshare.21835410.v1
title: Ten Years Assessment of Shifting Cultivation on Land Cover and Carbon Storage in Timor Island, Indonesia

description: Abstract The practice of shifting cultivation has led to forest degradation and deforestation in the Sisimeni Sanam Forest Area, with Special Purpose (SSFAwSP). Therefore, this study aims to assess land cover and carbon storage changes of the practiced shifting cultivation in SSFAwSP over the last ten years using 2013, 2016, and 2021 Landsat imagery. A hybrid classification approach that combines the forest canopy density model and supervised classification of maximum likelihood was used to create land cover maps to detect changes in forest land cover and carbon storage. The results showed that for ten years, the extent and annual rates of deforestation, forest degradation, forest regrowth, and changes in carbon storage were 662.62 ha (4%), 319.18 ha (3%), 163.8 ha (1%), and -54.51 kilo Ton C (3%), respectively. This finding indicated that shifting cultivation contributed only 10% and 1% of total deforestation and forest degradation, respectively.
DOI: 10.5281/zenodo.3528008
title: Using the Tea Bag Index to unravel how interactions between an antibiotic (Trimethoprim) and endocrine disruptor (17a-estradiol) affect aquatic microbial activity: Supporting Dataset 1

description: The constant release of complex mixture of pharmaceuticals, including antimicrobials and endocrine disruptors, into the aquatic environment. These have the potential to affect aquatic microbial metabolism and alter biogeochemical cycling of carbon and nutrients. We used the Tea Bag Index (TBI) for decomposition within a series of contaminant exposure experiments to test how interactions between an antibiotic (trimethoprim) and endocrine disruptor (17a-estradiol) affects microbial activity in an aquatic system. The TBI is a citizen science tool used to test microbial activity by measuring the differential degradation of green and rooibos tea as proxies for labile and recalcitrant organic matter decomposition. Here we present the raw data on pharmaceutical exposures and the mass loss of the Rooibos and Green tea bags within the experiment. From Tea Bag mass loss we then calculated the Stabilisation Factor (S) and Initial Decomposition Rate of the labile organic matter fraction.
DOI: 10.5281/zenodo.4266328
title: Using the Tea Bag Index to unravel how interactions between an antibiotic (Trimethoprim) and endocrine disruptor (17a-estradiol) affect aquatic microbial activity: Supporting Dataset 1

description: The constant release of complex mixture of pharmaceuticals, including antimicrobials and endocrine disruptors, into the aquatic environment. These have the potential to affect aquatic microbial metabolism and alter biogeochemical cycling of carbon and nutrients. We used the Tea Bag Index (TBI) for decomposition within a series of contaminant exposure experiments to test how interactions between an antibiotic (trimethoprim) and endocrine disruptor (17a-estradiol) affects microbial activity in an aquatic system. The TBI is a citizen science tool used to test microbial activity by measuring the differential degradation of green and rooibos tea as proxies for labile and recalcitrant organic matter decomposition. Here we present the raw data on pharmaceutical exposures and the mass loss of the Rooibos and Green tea bags within the experiment. From Tea Bag mass loss we then calculated the Stabilisation Factor (S) and Initial Decomposition Rate of the labile organic matter fraction.
DOI: 10.6084/m9.figshare.21835411
title: Evaluation of the Properties of LaNiO3 Material Prepared by the Modified Proteic Method for Adsorption of Environmental Contaminants

description: LaNiO3 material was synthesized by modified proteic method, calcined at 900 °C/2 h in air atmosphere and was applied to remove environmental contaminants. LaNiO3 phase with a perovskite structure was formed in the calcined material, which has a surface area of 22 m2 g-1 and is formed by fine particles agglomerated, with pH of the zero charge point of 7.44. The removal of a model pollutant (Turquoise Blue dye) indicated that the better removal occurs in acidic pH and with adsorbent’s mass of 50 mg, showing average value around 90% of removal efficiency and 131 mg g-1 for adsorption capacity (q) value. The variable constants model was the one that best fit the kinetic. LaNiO3 material resisted the test conditions and the recovery process, and maintained its high adsorption capacity throughout the six regeneration cycles studied, which indicates that it is a promising material as an adsorbent of pollutants and does not generate waste during the process.
DOI: 10.6084/m9.figshare.21835411.v1
title: Evaluation of the Properties of LaNiO3 Material Prepared by the Modified Proteic Method for Adsorption of Environmental Contaminants

description: LaNiO3 material was synthesized by modified proteic method, calcined at 900 °C/2 h in air atmosphere and was applied to remove environmental contaminants. LaNiO3 phase with a perovskite structure was formed in the calcined material, which has a surface area of 22 m2 g-1 and is formed by fine particles agglomerated, with pH of the zero charge point of 7.44. The removal of a model pollutant (Turquoise Blue dye) indicated that the better removal occurs in acidic pH and with adsorbent’s mass of 50 mg, showing average value around 90% of removal efficiency and 131 mg g-1 for adsorption capacity (q) value. The variable constants model was the one that best fit the kinetic. LaNiO3 material resisted the test conditions and the recovery process, and maintained its high adsorption capacity throughout the six regeneration cycles studied, which indicates that it is a promising material as an adsorbent of pollutants and does not generate waste during the process.
DOI: 10.6084/m9.figshare.21835413.v1
title: Anti-proliferative effects of paroxetine alone or in combination with sorafenib in HepG2 cells

description: Abstract Hepatocellular carcinoma (HCC) is a common cause of cancer-related death. Sorafenib is the first approved drug for the treatment of advanced HCC. Depression is frequent in cancer patients. Moreover, sorafenib might exert depression as an adverse drug reaction and paroxetine, a selective serotonin reuptake inhibitor, is a recommended pharmacotherapy. This study aimed to investigate the potential synergistic effects of paroxetine and sorafenib on HepG2 cell proliferation and death. Paroxetine and sorafenib were administered to HepG2 cells as single-agents or in combination. Cell viability was determined with XTT cell viability assay. Cellular apoptosis and DNA content were assessed by flow cytometry. The expression of anti-apoptotic Bcl-2 was examined by immunofluorescence confocal microscopy. A lower dose of sorafenib was found to be required to inhibit cell proliferation when in combination with paroxetine. Similarly, the coadministration enhanced cellular apoptosis and resulted in cell cycle arrest. Confocal imaging revealed a remarkably lower cell density and increased expression of Bcl-2 following combined treatment of paroxetine with sorafenib. To our knowledge, this is the first study demonstrating the synergistic effect of paroxetine and sorafenib in HCC and might provide a potentially promising therapeutic strategy.
DOI: 10.6084/m9.figshare.21835413
title: Anti-proliferative effects of paroxetine alone or in combination with sorafenib in HepG2 cells

description: Abstract Hepatocellular carcinoma (HCC) is a common cause of cancer-related death. Sorafenib is the first approved drug for the treatment of advanced HCC. Depression is frequent in cancer patients. Moreover, sorafenib might exert depression as an adverse drug reaction and paroxetine, a selective serotonin reuptake inhibitor, is a recommended pharmacotherapy. This study aimed to investigate the potential synergistic effects of paroxetine and sorafenib on HepG2 cell proliferation and death. Paroxetine and sorafenib were administered to HepG2 cells as single-agents or in combination. Cell viability was determined with XTT cell viability assay. Cellular apoptosis and DNA content were assessed by flow cytometry. The expression of anti-apoptotic Bcl-2 was examined by immunofluorescence confocal microscopy. A lower dose of sorafenib was found to be required to inhibit cell proliferation when in combination with paroxetine. Similarly, the coadministration enhanced cellular apoptosis and resulted in cell cycle arrest. Confocal imaging revealed a remarkably lower cell density and increased expression of Bcl-2 following combined treatment of paroxetine with sorafenib. To our knowledge, this is the first study demonstrating the synergistic effect of paroxetine and sorafenib in HCC and might provide a potentially promising therapeutic strategy.
DOI: 10.6084/m9.figshare.21835416
title: What aspects influence the prioritization of Primary Health Care by municipal managers in Rio Grande do Sul - Brazil?

description: Abstract Primary Health Care (PHC) is capable of achieving broad coverage and effectiveness. This study evaluated the factors associated with the prioritization that municipal health managers in the state of Rio Grande do Sul (RS) give to PHC. A cross-sectional analytical study was carried out between August 2017 and May 2019 using an online FormSUS questionnaire, consisting of 44 questions divided into 4 blocks, sent to 497 municipal health managers. Multiple logistic regression was performed to analyze factors associated with PHC prioritization. Out of 404 participants, 66.8% reported a maximum degree of prioritization. Managers with a postgraduate degree showed greater prioritization than those with elementary education (78.5% vs 58.8%, p&lt;0.01). Regarding the postgraduate area, managers with training in public health prioritized PHC more than in other areas, or with no postgraduate studies, respectively with 84%, 77.1% and 61.8% (p&lt;0.01). After adjusting for covariates, having a postgraduate degree in public health or in another area remained associated with greater chances of prioritization. The results may encourage initiatives that consider the level and area of education for this position focusing on technical, and not strictly political criteria.