DOI: 10.5281/zenodo.16743764
title: Dataset supporting publication: "Systematic review and meta-analysis of methodological approaches for characterising airborne SARS-CoV-2 RNA for environmental surveillance"

description: Dataset supporting the publication: "Systematic review and meta-analysis of methodological approaches for characterising airborne SARS-CoV-2 RNA for environmental surveillance" in the journal npj Climate and Atmospheric Science. Contains 11 data sheets.

Contains the following information on the studies included in the review:

General information on the studies: identificative information on the studies



Reference with link to publication

Reference

Country of study

Date of measurement (or publication if measurement not stated)


Information regarding sampling parameters: environments and microenvironments sampled, proximity to COVID-19 infected individuals, air volume sampled, sampling duration, airflow rate, filter type, sample transportation and storage conditions, and the recording of additional parameters during sampling. 



Indoor or Outdoor measurement

Type of environment settings: Healthcare, Socio-health, Transport, Other Indoors, Outdoor

Scope: Brief description of microenvironment location where sampling took place

Sites: Specific information on sampling sites, including number of sites where specified

Sampling near patients (yes/no)

Results colour code: Green (no detected), Yellow (Detected), Red (Detected and Quantified in at least one of the microenvironments sampled in the study).

Description of Sampler used to collect samples

Volume of air sampled

Duration of sampling

Air flow rate

Collection mechanism

Type of filters

Samples storage

Other parameters


Molecular analysis parameters for SARS-CoV-2 RNA characterisation: use of internal controls, recovery rate of internal standards, targeted genes, criteria used to consider a sample positive for detection, and Limits of detection (LoD) and quantification (LoQ).



Internal standards used

Internal standards (Recovery)

Genes analysed

Definition of positive sample

LoD, LoQ


Information on characterisation results: detection, positivity rate, quantification, other relevant results



RNA Detected (Yes/No) 

Positivity rate (positive samples/total air samples) 

RNA quantification (Yes/No)

RNA viral load (copies/m3), range: (minimum maximum)

SARS-CoV-2 viability (Yes, No, Not studied)  

SARS-CoV-2 viability  

Results


Variables included in each datasheet are listed below.

DATASHEET 1: All



Reference (with links to original publication)   

Reference    

Country 

Date    

Indoor_Outdoor    

Healthcare settings    

Socio-health settings    

Transport Settings    

Other indoor settings   

 Outdoor settings    

Scope   

Sites   

Sampling near COVID-19 patients    

Results Colour code (All)    

Sampler   

Reference    

Volume of air sampled    

Duration of sampling    

Air flow rate    

Collection mechanism   

Type of filters   

Samples storage    

Other parameters    

Internal satndards    

nternal standards (Recovery)   

Genes analysed    

Definition of positive sample   

LoD, LoQ    

RNA Detected (Yes/No)    

Positivity rate (positive samples/total air samples) only filters   

RNA quantification (Yes/No)    

RNA viral load (copies/m3), range: (minimum maximum)   

SARS-CoV-2 viability (Yes, No, Not studied)    

SARS-CoV-2 viability    

Results


DATASHEET 2: Search

Contains information on the reference and whether sampling was conducted near COVID-19 patients. This dataset is used to select studies that are conducted near patients in the data analysis included in the review



Reference    

Sampling near COVID-19 patients


Datasheets 3-11 contain information extracted from the first datasheet and used to evaluate several aspects included in the review.

DATASHEET 3: Viral load



Indoor_Outdoor

Reference

Sampling near COVID-19 patients

RNA Detected (Yes/No)

RNA quantification (Yes/No)

RNA viral load (copies/m3), range: (minimum maximum)  

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3


DATASHEET 4: Volume Viral load



Reference

Volume

Environment

RNA Detected (Yes/No)

RNA quantification (Yes/No)            

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3    

Near COVID-19 person


 DATASHEET 5: Time Viral load



Reference       

environment    

Duration of sampling  time     

RNA Detected (Yes/No)        

RNA quantification (Yes/No)            

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3    

Sampling near COVID-19 patients


DATASHEET 6: Flow Viral load



Reference       

environment    

Air flow rate   

Air_flow         

RNA Detected (Yes/No)        

RNA quantification (Yes/No)            

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3    

Sampling near COVID-19 patients


DATASHEET 7: Gen Viral load



Reference       

Indoor_Outdoor         

RNA Detected (Yes/No)        

RNA quantification (Yes/No)            

Genes analysed           

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)

mean_viral load copies/m3


DATASHEET 8: Filter type Viral load



Indoor_Outdoor         

Reference       

Sampling near COVID-19 patients   

filter    

filter_2            

RNA Detected (Yes/No)        

RNA quantification (Yes/No)            

RNA viral load, range: minimum maximum)           

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3


DATASHEET 9: Storage Viral load



Indoor_Outdoor         

Reference       

Sampling near COVID-19 patients   

Samples storage          

Storage           

transport_cold

RNA Detected (Yes/No)        

RNA quantification (Yes/No)            

RNA viral load, range: minimum maximum)           

Storage_2       

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3


DATASHEET 10: Environments



Reference       

Sampling near COVID-19 patients   

Environments 

RNA Detected (Yes/No)

Positivity rate (positive samples/total air samples)

RNA quantification (Yes/No)            

RNA viral load, range: minimum maximum)           

Positive Filters            

Total filters     

viral load  (copies/m3,min)     

viral load (copies/m3,max)     

mean_viral load copies/m3


DATASHEET 11: Years



Indoor_Outdoor

Reference (with links to original publication)      

Reference       

Year   

Country           

Date    

Healthcare settings     

Socio-health settings  

Transport Settings      

Other indoor settings  

Outdoor settings         

Scope  Sites    

Sampling near COVID-19 patients

Results (All)   

Sampler           

Reference       

Volume of air sampled           

Duration of sampling  

Air flow rate   

Collection mechanism            

Type of filters 

Samples storage          

Other parameters        

Internal satndards       

Internal standards (Recovery)

Genes analysed           

Definition of positive sample 

LoD, LoQ       

RNA Detected (Yes/No)

Positivity rate (positive samples/total air samples) only filters

RNA quantification (Yes/No)

RNA viral load, range (minimum maximum)

SARS-CoV-2 viability (Yes, No, Not studied)

SARS-CoV-2 viability