DOI: 10.35077/ace-oak-dig
title: Confocal imaging of female P14 Ooceraea biroi whole antennae

description: The following data collection comprises several experiments that use confocal fluorescence microscopy and smRNA FISH to investigate
 the transcriptional mechanism of olfactory receptors (ORs) expression in Ooceraea biroi antennae. Experiments were carried out in
 whole antennae in which olfactory sensory neurons were stained with custom RNA FISH probes. These data demonstrate that
 bidirectional transcription occurs leading to 1) inhibition of transcription of upstream ORs in a tandem array and 2) non-canonical
 readthrough producing non-translated transcripts from ORs downstream of the initiation site.
description: Each subdirectory contains individual antennae stained for distinct genes. These names correspond to the data included in our
 publication. The target of each probe is specified in the subdirectory name (U34, Chymotrypsin, Lnc4, T79-intergenic, 9E88, etc.).
 The numbers 488, 546, 594, 647 correspond to the Alexa fluorophores used for staining and imaging. Therefore, “U34-B3-546” indicates
 that U34 is in the red Alexa546 channel. All samples are age-matched to P14 (pupal day 14). Each TIFF image represents a distinct
 biological replicate of the same stain.
 DATASET METADATA:
 title:'Confocal imaging of female P14 Ooceraea biroi whole antennae ',
 GeneralModality:'cell morphology',
 Technique:'smFISH'
 SPECIMEN METADATA:
 LocalID:'20250307_B1_P14_U34-B3-546_Chymotrypsin-B2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250307_B1_P14_U34-B3-546_Chymotrypsin-B2-647_DAPI',
 LocalID:'20250325_4_P14_U34-B3-488_lnc4-B1-546_U21-B5-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250325_4_P14_U34-B3-488_lnc4-B1-546_U21-B5-647_DAPI',
 LocalID:'20250325_5_P14_G1-b1-546_lnc7-B2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250325_5_P14_G1-b1-546_lnc7-B2-647_DAPI',
 LocalID:'20250325_6_P14_9E198-B3-488_9E197-B1-546_9E196-B2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250325_6_P14_9E198-B3-488_9E197-B1-546_9E196-B2-647_DAPI',
 LocalID:'20250328_1_P14_T79-intergenic-b2-647_T79-exonic-b1-546_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250328_1_P14_T79-intergenic-b2-647_T79-exonic-b1-546_DAPI',
 LocalID:'20250328_2_P14_R2-b3-488_Q1-b1-546_Lnc6-b2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250328_2_P14_R2-b3-488_Q1-b1-546_Lnc6-b2-647_DAPI',
 LocalID:'20250328_3_P14_Lnc3-b3-488_L16-b2-594_Lnc2-b5-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250328_3_P14_Lnc3-b3-488_L16-b2-594_Lnc2-b5-647_DAPI',
 LocalID:'20250328_4_P14_9E129-b3-488_LOC104-b1-546_9E116-b2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250328_4_P14_9E129-b3-488_LOC104-b1-546_9E116-b2-647_DAPI',
 LocalID:'20250328_5_P14_LOC603-b3-488_9E108-b1-546_9E116-b2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250328_5_P14_LOC603-b3-488_9E108-b1-546_9E116-b2-647_DAPI',
 LocalID:'20250508_P14_T_9E89-b1-546_9E99-B2-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250508_P14_T_9E89-b1-546_9E99-B2-647_DAPI',
 LocalID:'20250508_P14_T_9E99-b2-594_LOC105286072-B5-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250508_P14_T_9E99-b2-594_LOC105286072-B5-647_DAPI',
 LocalID:'20250523_A_P14_T_9E213-546_9E214-488_Intergenic-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250523_A_P14_T_9E213-546_9E214-488_Intergenic-647_DAPI',
 LocalID:'20250523_B_P14_T_9E88-546_Lnc-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250523_B_P14_T_9E88-546_Lnc-647_DAPI',
 LocalID:'20250523_C_P14_T_U54-647_Lnc4-546_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250523_C_P14_T_U54-647_Lnc4-546_DAPI',
 LocalID:'20250701_A_P14_9E118-EXONS-B1-546_9E118-INTRONS-B2-647_9E129-B3-488_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250701_A_P14_9E118-EXONS-B1-546_9E118-INTRONS-B2-647_9E129-B3-488_DAPI',
 LocalID:'20250701_F_P14_U34-b3-546_Orco-b4-647_DAPI',
 Species:'Ooceraea biroi',
 NCBITaxonomy:'NCBI:txid2015173',
 Age:'14 day pupae',
 Ageunit:'Days',
 Sex:'Female',
 SampleLocalID:'20250701_F_P14_U34-b3-546_Orco-b4-647_DAPI'
 INSTRUMENT METADATA:
 MicroscopeType:'Confocal',
 MicroscopeManufacturerAndModel:'Zeiss LSM 900',
 ObjectiveName:'Zeiss LD LCI Plan-Apochromat',
 ObjectiveImmersion:'Glycerol',
 ObjectiveNA:'1.2NA',
 ObjectiveMagnification:'40x',
 DetectorType:'PMT'
 IMAGE METADATA:
 xAxis:'NA',
 yAxis:'NA',
 zAxis:'NA',
 Number:'1, 2 ,3, 4, magenta, yellow, cyan, grey',
 displayColor:'(1.0, 0.0, 1.0), (1.0, 1.0, 0.0), (0.0, 1.0, 1.0), (0.5, 0.5, 0.5)',
 stepSizeX:'0.09748',
 stepSizeY:'0.09748',
 Slices:'100(approx)'