DOI: 10.5061/dryad.9cnp5hqfj
title: GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation

description: Osteoporosis is a common systemic skeletal disorder resulting in bone
 fragility and increased fracture risk. It is still necessary to explore
 its detailed mechanisms and identify novel targets for the treatment of
 osteoporosis. Previously, we found that an lncRNA named GAS5 in humans
 could negatively regulate lipoblast/adipocyte differentiation. However, it
 is still unclear whether GAS5 affects osteoblast differentiation and
 whether GAS5 is associated with osteoporosis. Our current research found
 that GAS5 was decreased in the bones and BMSCs, a major origin of
 osteoblast, in osteoporosis patients. Mechanistically, GAS5 promotes
 osteoblast differentiation by interacting with UPF1 to degrade Smad7 mRNA.
 Moreover, a decreased bone mass and impaired bone repair ability were
 observed in Gas5 heterozygous mice, manifesting in osteoporosis. The
 systemic supplement of Gas5-overexpressing adenoviruses significantly
 ameliorated bone loss in an osteoporosis mouse model. In conclusion, GAS5
 promotes osteoblast differentiation by targeting the UPF1/Smad7 axis and
 protects against osteoporosis.
description: This dataset was obtained through various experiments, and
 statistical graphs were obtained after statistical analysis by SPSS
 software.
description: # GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in
 osteoblast differentiation
 [https://doi.org/10.5061/dryad.9cnp5hqfj](https://doi.org/10.5061/dryad.9cnp5hqfj) By studying the GAS5 gene in MSCs cells and animals, we found that GAS5 can promote MSCs osteoblast differentiation and protect against osteoporosis by targeting UPF1/SMAD7. This dataset includes the study data and original images of the research. ## Description of the data and file structure This dataset is divided according to each Figure. The raw data for each Figure corresponds separately to the folder in it. Images of the supplementary data included in the study are also presented in this dataset. #### Staining Data * ALP: alkaline phosphatase staining * ARS: alizarin red staining * HE: hematoxylin-eosin staining * MASSON: Masson staining * COL1: COL1A1 immunohistochemical staining * OCN: BGLAP or Bglap immunohistochemical staining #### Statistic Data The statistical data of each experiment have been put in Excel tables. The information represented by the variables is as follows: Figure 1: * Normal: Normal population * Dysplasia: Patient with developmental dysplasia of the hip * Osteoporosis: Patient with osteoporosis * Absorbance: The absorbance of alizarin red stain after extraction * Cyto: cytoplasm * Nu: nucleus Figure 2: shCtrl/shGAS5-1/shGAS5-2 represent controls, GAS5 knocks down viruses 1 and 2, respectively. Figure 3: Vector/OE-GAS5 represents an empty vector, and GAS5 overexpresses lentiviruses, respectively. Figure 4: Flag-FL/Flag-CH/Flag-HD/Flag-CH-HD/Flag-HD-SQ represent different segment structure of UPF1. Figure 5: si stands for knocked down siRNA of different molecules, and OE stands for overexpression of different molecules. Figure 6: GAS5+/+ represents the wild mouse, and GAS5+/- stands for knocking out heterozygotes. Figure 7: NS. + Vector means the group of normal saline and empty vector, DXMS + Vector means the group of dexamethasone and empty vector, DXMS+GAS5-OE means the group of dexamethasone and GAS5 overexpression adenoviruses.  The variables in the supplementary figure are consistent with those described above. ## Sharing/Access information There are no other externally accessible datasets for this dataset. ## Code/Software No special code or software is involved in this dataset.