DOI: 10.5061/dryad.ks48tt4
title: Data from: Expression of matrix Metalloproteinases-2 and aquaporin-1 in corneoscleral junction after angle-closure in rabbits

description: Background: To investigate the expression of Matrix Metalloproteinases 2
 and aquaporin-1 in corneoscleral junction and explore the mechanism of
 trabecular damageafter angle-closure. Methods: Thirty New Zealand white
 rabbits were randomly assigned into 2 groups, theexperimental group (Group
 1) including twenty five rabbits and the control group (Group 2) including
 5 rabbits. The rabbits in the experimental group were used to establish
 angle-closure models, and the rabbits in the control group were not
 subjected to any operation. All the rabbits were followed by slit lamp
 microscopy, Tonopen tonometer, and anterior segment optical coherent
 tomography (AS-OCT). The expressions of metalloproteinase MMP-2,
 aquaporin-1, and tissue inhibitors of metalloproteinase-2 in corneoscleral
 junctionwere evaluatedin both groups byimmunofluorescence, quantitative
 reverse-transcription polymerase chain reaction (qRT-PCR), and
 enzyme-linked immunosorbent assay (ELISA). Results: Slit-lamp examination
 showed that angle-closure model was successfully established in twenty
 rabbits. The extent of angle-closure was about 2 to 4 clock hours in all
 the rabbit models, but the intraocular pressure (IOP) of the rabbits
 distributed from 8.57 to 15.25 mmHg and no significant high IOP was found
 in the follow-up period. The AQP-1-positive cells mainly located in
 Schlemm’s canal, the inner surface of trabecular meshwork (TM), and the
 surface of iris, which began to decline on 1 month after angle-closure.
 MMP2 staining was diffuse in trabecular meshwork and iris.
 Immunofluorescence signal of MMP2 was strong within 1 month after
 angle-closure, and subsequently became weak. qRT-PCR and ELISA showed that
 the expression of MMP-2 and TIMP-2 increased within 1 month after
 angle-closure and then declined gradually. The AQP-1 levels showed
 slightly declined on 1 month after angle-closure. Conclusions: Altered
 levels of MMPs, TIMPs, and AQP-1 were found in the area of angle-closure,
 which may be involved in the damage of TM and Schlemm’s canal after
 angle-closure. .
description: Slit-lamp photography of
 nomal rabbit's eyeFigure
 2ANormal-裂隙灯1.jpgSlit-lamp photography of nomal rabbit's eye 2Normal-裂隙灯2.jpgSlit-lamp photography of the rabbit with angle-closureFig.2Atu_zi1223_OD_2018-05-14_19-06-56_026.jpgSlit-lamp photography of the rabbit with angle-closure2Fig.2Bjiang_OS_2018-05-14_19-09-46_012.jpgAnterior segment OCT of rabbit with angle-closure12.jpgHE stain of normal rabbit's eyeNormal-1.tifHE stain of rabbit's eye with angle-closure on postoperative 1 week1W.tifHE stain of rabbit's eye with angle-closure on postoperative 1 month1m.tifHE stain of rabbit's eye with angle-closure on postoperative 2 months2m.tifHE stain of rabbit's eye with angle-closure on postoperative 4 months4m.tifControl for immunofluorescence staining of AQP-1AQP-control.tifImmunofluorescence staining of AQP-1 in normal rabbit's eyeAQP-Normal.tifImmunofluorescence staining of AQP-1 in the rabbit's eye at 1 month after angle-closureAQP-1m.tifImmunofluorescence staining of AQP-1 in the rabbit's eye at 4 months after angle-closureAQP-4m.tifControl for immunofluorescence staining of MMP-2MMP-control.tifImmunofluorescence staining of MMP-2 in normal rabbit's eyeMMP-normal.tifImmunofluorescence staining of MMP-2 in the rabbit's eye at 1 month after angle-closureMMP-1m.tifImmunofluorescence staining of MMP-2 in the rabbit's eye at 4 months after angle-closureMMP-4m.tifELISA raw dataELISA.xlsxRaw data of intraocular pressureIOP.xlsxRaw data of RT-PCRFig5-PCR.xlsx