cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6743528
title: Supplementary Material for: Let-7d Inhibits Growth and Metastasis in Breast Cancer by Targeting Jab1/Cops5

description: <b><i>Background/Aims:</i></b> MicroRNAs (miRNAs) regulate the expressions of cancer-related genes, and are involved in the development and progression of various human cancers. Here, we performed further analyses to determine whether let-7d is functionally linked to Jab1 in breast cancer. <b><i>Methods:</i></b> In situ hybridization and immunohistochemical analyses were used to determine the level of let-7d and Jab1 in breast cancer clinical specimens and its correlation with clinicopathological data. Let-7d overexpressing breast cancer cell lines combined with mouse models bearing cell-derived xenografts were used to assess the functional role of let-7d both <i>in vitro</i> and <i>in vivo</i>. <b><i>Results:</i></b> In this study, we found that let-7d was downregulated in breast cancer tissues, coupled with the elevations of Jab1 protein expressions, compared with paired adjacent noncancerous breast tissues. Let-7d overexpression significantly suppressed the proliferation and invasion in MCF-7 and MDA-MB-231 cells. Dual luciferase reporter assay indicated that Jab1 was the direct target of let-7d. Stepwise studies from <i>in vitro</i> and <i>in vivo</i> experiments indicated that let-7d overexpression inhibited cell growth and decreased Jab1 expressions in breast cancer cells and nude mice tumor tissues. Statistical analyses demonstrated that breast cancer patients with low levels of let-7d or high levels of Jab1 had a significant correlation with worse prognosis. <b><i>Conclusion:</i></b> These findings provide novel insights into molecular mechanism of let-7d and Jab1 in tumor development and progression of breast cancer, and thus let-7d/Jab1 are novel potential therapeutic targets for breast cancer patients.
DOI: 10.6084/m9.figshare.6743528.v1
title: Supplementary Material for: Let-7d Inhibits Growth and Metastasis in Breast Cancer by Targeting Jab1/Cops5

description: <b><i>Background/Aims:</i></b> MicroRNAs (miRNAs) regulate the expressions of cancer-related genes, and are involved in the development and progression of various human cancers. Here, we performed further analyses to determine whether let-7d is functionally linked to Jab1 in breast cancer. <b><i>Methods:</i></b> In situ hybridization and immunohistochemical analyses were used to determine the level of let-7d and Jab1 in breast cancer clinical specimens and its correlation with clinicopathological data. Let-7d overexpressing breast cancer cell lines combined with mouse models bearing cell-derived xenografts were used to assess the functional role of let-7d both <i>in vitro</i> and <i>in vivo</i>. <b><i>Results:</i></b> In this study, we found that let-7d was downregulated in breast cancer tissues, coupled with the elevations of Jab1 protein expressions, compared with paired adjacent noncancerous breast tissues. Let-7d overexpression significantly suppressed the proliferation and invasion in MCF-7 and MDA-MB-231 cells. Dual luciferase reporter assay indicated that Jab1 was the direct target of let-7d. Stepwise studies from <i>in vitro</i> and <i>in vivo</i> experiments indicated that let-7d overexpression inhibited cell growth and decreased Jab1 expressions in breast cancer cells and nude mice tumor tissues. Statistical analyses demonstrated that breast cancer patients with low levels of let-7d or high levels of Jab1 had a significant correlation with worse prognosis. <b><i>Conclusion:</i></b> These findings provide novel insights into molecular mechanism of let-7d and Jab1 in tumor development and progression of breast cancer, and thus let-7d/Jab1 are novel potential therapeutic targets for breast cancer patients.
DOI: 10.6084/m9.figshare.6743522
title: Communication Based Control for DC Microgrids

description: Centralized communication-based control is one of the main methods that can be implemented to achieve autonomous advanced energy management capabilities in DC microgrids. However, its major limitation is the fact that communication bandwidth and computation resources are limited in practical applications. This can be often improved by avoiding redundant communications and complex computations. In this paper, an autonomous communication-based hybrid state/event driven control scheme is proposed. This control scheme is hierarchical and heuristic, such that on the primary control level, it encompasses state-driven local controllers, and on the secondary control level, an event-driven MG centralized controller (MGCC) is used. This heuristic hybrid control system aims at reducing the communication load and complexity, processor computations, and consequently system cost while maintaining reliable autonomous operation during all possible scenarios. A mathematical model for the proposed control scheme using Finite State Machines (FSM) has been developed and used to cover all the possible modes/sub-modes of operation, and assure seamless transitions among them during various events. Results of some case studies involving severe operational scenarios were presented and discussed. Results verify the validity and effectiveness of the proposed communication-based control scheme
DOI: 10.6084/m9.figshare.6743519
title: Supplementary Material for: Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

description: <b><i>Background/Aims:</i></b> Pancreatic cancer remains one of the deadliest human malignancies, the lethality of which may be attributed to the presence of pancreatic cancer stem cells (PCSCs), a small subpopulation of cells existing within pancreatic tumor with high carcinogenesis. Therefore, it is crucial to establish an efficient enrichment and culture system of PCSCs and identify the key genes involved in the regulation of PCSCs. The three-dimensional (3D) liquid suspension mammosphere culture system has been established for enrichment and culture of PCSCs <i>in vitro</i> as the cell spheres are likely to originate from individual cell clone, but it has been challenged because the cell spheroids could be a result of cell aggregation. <b><i>Methods:</i></b> We optimized the existing culture system by adding methylcellulose to create a 3D semi-solid system which prevented the non-specific aggregation. Then we identified the CSC properties of Panc-1 spheroid cells cultured by this system by detecting the genes associated with stemness and by evaluation of the tumorigenicity <i>in vitro</i> and <i>in vivo</i> through invasion, migration and xenograft experiments methods. Subsequently, we performed high-throughput sequencing (HTS) of the Panc-1 spheroid cells. <b><i>Results:</i></b> We confirmed the PCSCs properties and high malignancy of the Panc-1 spheroid cells enriched by our novel 3D semi-solid system both <i>in vitro</i> and <i>in vivo</i>. Hundreds of mRNA, microRNA (miRNA) and dozens of long non-coding RNA (LncRNA) were identified to be differentially regulated in PCSCs-like Panc-1 spheroid cells compared with their parental cells by HTS. <b><i>Conclusions:</i></b> Our results demonstrate an efficient enrichment and culture system for Panc-1 spheroid cells with the PCSCs properties. The differentially expressed genes and their targets identified by the HTS of the Panc-1 spheroid cells can serve as new potential biomarkers for pancreatic cancer diagnosis and targeted therapy.
DOI: 10.6084/m9.figshare.6743519.v1
title: Supplementary Material for: Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

description: <b><i>Background/Aims:</i></b> Pancreatic cancer remains one of the deadliest human malignancies, the lethality of which may be attributed to the presence of pancreatic cancer stem cells (PCSCs), a small subpopulation of cells existing within pancreatic tumor with high carcinogenesis. Therefore, it is crucial to establish an efficient enrichment and culture system of PCSCs and identify the key genes involved in the regulation of PCSCs. The three-dimensional (3D) liquid suspension mammosphere culture system has been established for enrichment and culture of PCSCs <i>in vitro</i> as the cell spheres are likely to originate from individual cell clone, but it has been challenged because the cell spheroids could be a result of cell aggregation. <b><i>Methods:</i></b> We optimized the existing culture system by adding methylcellulose to create a 3D semi-solid system which prevented the non-specific aggregation. Then we identified the CSC properties of Panc-1 spheroid cells cultured by this system by detecting the genes associated with stemness and by evaluation of the tumorigenicity <i>in vitro</i> and <i>in vivo</i> through invasion, migration and xenograft experiments methods. Subsequently, we performed high-throughput sequencing (HTS) of the Panc-1 spheroid cells. <b><i>Results:</i></b> We confirmed the PCSCs properties and high malignancy of the Panc-1 spheroid cells enriched by our novel 3D semi-solid system both <i>in vitro</i> and <i>in vivo</i>. Hundreds of mRNA, microRNA (miRNA) and dozens of long non-coding RNA (LncRNA) were identified to be differentially regulated in PCSCs-like Panc-1 spheroid cells compared with their parental cells by HTS. <b><i>Conclusions:</i></b> Our results demonstrate an efficient enrichment and culture system for Panc-1 spheroid cells with the PCSCs properties. The differentially expressed genes and their targets identified by the HTS of the Panc-1 spheroid cells can serve as new potential biomarkers for pancreatic cancer diagnosis and targeted therapy.
DOI: 10.6084/m9.figshare.6743498
title: datasets

description: Spectral dataset for grapevine varietal classification. Python source code for model training is also included.
DOI: 10.6084/m9.figshare.6743459
title: NWGC

description: Marine seawater metagenomes collected from Hong Kong Water, Red Sea, and Zhu Hai. These metagenomes were combined to generate a nonredundant gene catalogue, as new seawater gene catalogue.
DOI: 10.6084/m9.figshare.6743459.v1
title: NWGC

description: Marine seawater metagenomes collected from Hong Kong Water, Red Sea, and Zhu Hai. These metagenomes were combined to generate a nonredundant gene catalogue, as new seawater gene catalogue.
DOI: 10.6084/m9.figshare.6743468
title: Supplementary Material for: Egr-1 Maintains NSC Proliferation and Its Overexpression Counteracts Cell Cycle Exit Triggered by the Withdrawal of Epidermal Growth Factor

description: In adult mammals, neural stem cells (NSCs) reside in specialized niches at the level of selected CNS regions, such as the subventricular zone (SVZ). The signaling pathways that reg­ulate NSC proliferation and differentiation remain poorly understood. Early growth response protein 1 (Egr-1) is an important transcription factor, widely studied in the adult mammalian brain, mediating the activation of target genes by a variety of extracellular stimuli. In our study, we aimed at testing how Egr-1 regulates adult NSCs derived from mouse SVZ and, in particular, the interplay between Egr-1 and the proliferative factor EGF. We demonstrate that Egr-1 expression in NSCs is induced by growth factor stimulation, and its level decreases after EGF deprivation or by using AG1478, an inhibitor of the EGF/EGFR signaling pathway. We also show that Egr-1 overexpression rescues the cell proliferation decrease observed either after EGF removal or upon treatment with AG1478, suggesting that Egr-1 works downstream of the EGF pathway. To better understand this mechanism, we investigated targets downstream of both the EGF pathway and Egr-1, and found that they regulate genes involved in NSC proliferation, such as cell cycle regulators, cyclins, and cyclin-dependent kinase inhibitors.
DOI: 10.6084/m9.figshare.6743468.v1
title: Supplementary Material for: Egr-1 Maintains NSC Proliferation and Its Overexpression Counteracts Cell Cycle Exit Triggered by the Withdrawal of Epidermal Growth Factor

description: In adult mammals, neural stem cells (NSCs) reside in specialized niches at the level of selected CNS regions, such as the subventricular zone (SVZ). The signaling pathways that reg­ulate NSC proliferation and differentiation remain poorly understood. Early growth response protein 1 (Egr-1) is an important transcription factor, widely studied in the adult mammalian brain, mediating the activation of target genes by a variety of extracellular stimuli. In our study, we aimed at testing how Egr-1 regulates adult NSCs derived from mouse SVZ and, in particular, the interplay between Egr-1 and the proliferative factor EGF. We demonstrate that Egr-1 expression in NSCs is induced by growth factor stimulation, and its level decreases after EGF deprivation or by using AG1478, an inhibitor of the EGF/EGFR signaling pathway. We also show that Egr-1 overexpression rescues the cell proliferation decrease observed either after EGF removal or upon treatment with AG1478, suggesting that Egr-1 works downstream of the EGF pathway. To better understand this mechanism, we investigated targets downstream of both the EGF pathway and Egr-1, and found that they regulate genes involved in NSC proliferation, such as cell cycle regulators, cyclins, and cyclin-dependent kinase inhibitors.
DOI: 10.6084/m9.figshare.6743450
title: BSISO air-sea diagnostics package applied to MERRA2 reanalysis

description: Results of air-sea interaction diagnostic package for the boreal summer intraseasonal oscillation applied to the National Aeronautics and Space Administration Modern Era Retrospective analysis for Research and Applications, version 2 (MERRA2), for the period 1986-2016. The diagnostic package is described in DeMott et al. (2016) and Gao et al. (2018).<br>The package contains a set of netCDF files, one from each stage of the diagnostic code. There are three levels of diagnostics (L1, L2, L3), with multiple stages at each level. More information can be found in DeMott et al. (2016), linked below.
DOI: 10.6084/m9.figshare.6743450.v2
title: BSISO air-sea diagnostics package applied to MERRA2 reanalysis

description: Results of air-sea interaction diagnostic package for the boreal summer intraseasonal oscillation applied to the National Aeronautics and Space Administration Modern Era Retrospective analysis for Research and Applications, version 2 (MERRA2), for the period 1986-2016. The diagnostic package is described in DeMott et al. (2016) and Gao et al. (2018).<br>The package contains a set of netCDF files, one from each stage of the diagnostic code. There are three levels of diagnostics (L1, L2, L3), with multiple stages at each level. More information can be found in DeMott et al. (2016), linked below.
DOI: 10.6084/m9.figshare.6743450.v1
title: BSISO air-sea diagnostics package applied to MERRA2 reanalysis

description: Results of air-sea interaction diagnostic package for the boreal summer intraseasonal oscillation applied to the National Aeronautics and Space Administration Modern Era Retrospective analysis for Research and Applications, version 2 (MERRA2), for the period 1986-2016. The diagnostic package is described in DeMott et al. (2016) and Gao et al. (2018).<br>The package contains a set of netCDF files, one from each stage of the diagnostic code. There are three levels of diagnostics (L1, L2, L3), with multiple stages at each level. More information can be found in DeMott et al. (2016), linked below.
DOI: 10.6084/m9.figshare.6743429
title: BSISO air-sea diagnostics package applied to ERA-Interim reanalysis

description: Results of air-sea interaction diagnostic package for the boreal summer intraseasonal oscillation applied to the European Centre for Medium-range Weather Forecasts Interim (ERA-Interim) reanalysis data, for the period 1986-2016. The diagnostic package is described in DeMott et al. (2016) and Gao et al. (2018). <br>The package contains a set of netCDF files, one from each stage of the diagnostic code. There are three levels of diagnostics (L1, L2, L3), with multiple stages at each level. More information can be found in DeMott et al. (2016), linked below.
DOI: 10.6084/m9.figshare.6743429.v1
title: BSISO air-sea diagnostics package applied to ERA-Interim reanalysis

description: Results of air-sea interaction diagnostic package for the boreal summer intraseasonal oscillation applied to the European Centre for Medium-range Weather Forecasts Interim (ERA-Interim) reanalysis data, for the period 1986-2016. The diagnostic package is described in DeMott et al. (2016) and Gao et al. (2018). <br>The package contains a set of netCDF files, one from each stage of the diagnostic code. There are three levels of diagnostics (L1, L2, L3), with multiple stages at each level. More information can be found in DeMott et al. (2016), linked below.
DOI: 10.6084/m9.figshare.6743429.v2
title: BSISO air-sea diagnostics package applied to ERA-Interim reanalysis

description: Results of air-sea interaction diagnostic package for the boreal summer intraseasonal oscillation applied to the European Centre for Medium-range Weather Forecasts Interim (ERA-Interim) reanalysis data, for the period 1986-2016. The diagnostic package is described in DeMott et al. (2016) and Gao et al. (2018). <br>The package contains a set of netCDF files, one from each stage of the diagnostic code. There are three levels of diagnostics (L1, L2, L3), with multiple stages at each level. More information can be found in DeMott et al. (2016), linked below.
DOI: 10.6084/m9.figshare.6743348
title: Intervention stuyd_primary care_educational outreach vistis for knee osteoarthritis

description: This file contains the original dataset to reproduce the outcomes
DOI: 10.6084/m9.figshare.6743354
title: Susceptibility of different Drosophila melanogaster DGRP lines to three sigma viruses.

description: We infected 32 lines from the Drosophila Genetic Reference Panel (DGRP) that were susceptible for both p62 (Ref2P) and CHKov (n=11 lines), were resistant for CHKov only (n=13 lines), or were resistant for both genes (n=8 lines) with DMelSV, DAffSV and DObsSV. No lines in the panel were resistant for p62 only. We infected a mean of 18 flies per line (range = 3-22).<br><br><br>ref2p_chkov coding as follows("0_0", "0_1", "1_1") == ("Both sus", "Ref2P susceptible CHKov resistant", "Both resistant")
DOI: 10.6084/m9.figshare.6743345
title: EFFECT OF TORCH AGENTS AND CHLAMYDIA TRACHOMATIS ON REPRODUCTIVE.pdf

description: simplex virus 2 (HSV2)] and Chlamydia trachomatis on semen parameters and fertility hormones; follicular stimulating hormone (FSH); luteinizing hormone (LH); prolactin; progesterone; testosterone among infertile males.Methods: Serum and semen samples collected from (155) infertile males. Enzyme-linked immunosorbent assay test used to determine TORCH and C. trachomatis immunoglobulin M (IgM), FSH, LH, prolactin; progesterone; testosterone among infertile males. Standard world health organization protocol used to perform semen analysis.Results: A significant correlation between age group and CMV - IgM, HSV2 - IgM positivity (p=0.05 and 0.024). Type of infertility inversely correlated with T. gondii - IgM positivity (p=0.039) and positively correlated with Rubella - IgM (p=0.021). Significant correlation of T. gondii - CMV co-infection (p=0.048); T. gondii - Rubella co-infection (p=0.007); T. gondii - C. trachomatis co-infection (p=0.001). Significant correlation between semen parameters and TORCH positivity testosterone level significantly correlated with T. gondii - IgM; Rubella - IgM. Inverse significant correlation between C. trachomatis - IgM and FSH level. Significant correlations between TORCH - IgM positivity, semen parameters and hormones were following T. gondii, CMV, total sperm count and LH. T. gondii, Rubella, active motility and LH. T. gondii, total sperm count and prolactin. CMV, HSV2, C. trachomatis trachomatis, active sperm motility and progesterone. Rubella, sluggish motility and LH level. T. gondii; Rubella, sperm dead % and LH. T. gondii; sluggish motility and FSH. T. gondii; CMV; sluggish motility and FSH. T. gondii, sluggish motility and prolactin.HSV2, sluggish motility and progesterone level. T. gondii, CMV, HSV2, sperm dead % and progesterone. Sluggish motility, T. gondii, C. trachomatis, HSV2 and testosterone. T. gondii, C. trachomatis, sperm dead % and testosterone. C. trachomatis, HSV2; normal sperm morphology and LH. T. gondii, normal sperm morphology and prolactin level. HSV2, normal sperm morphology and testosterone. T. gondii, abnormal sperm morphology and prolactin. C. trachomatis, abnormal sperm morphology and LH.Conclusions: A current study proved several important and outstanding results about the role of TORCH agent and C. trachomatis in male infertility. TORCH agent and C. trachomatis have direct effects on sperm parameters. T. gondii and Rubella have a direct effect on testosterone levels; C. trachomatis has direct effect on FSH among infertile males. TORCH agent plays a
DOI: 10.6084/m9.figshare.6743348.v2
title: Intervention stuyd_primary care_educational outreach vistis for knee osteoarthritis

description: This file contains the original dataset to reproduce the outcomes
DOI: 10.6084/m9.figshare.6743345.v1
title: EFFECT OF TORCH AGENTS AND CHLAMYDIA TRACHOMATIS ON REPRODUCTIVE.pdf

description: simplex virus 2 (HSV2)] and Chlamydia trachomatis on semen parameters and fertility hormones; follicular stimulating hormone (FSH); luteinizing hormone (LH); prolactin; progesterone; testosterone among infertile males.Methods: Serum and semen samples collected from (155) infertile males. Enzyme-linked immunosorbent assay test used to determine TORCH and C. trachomatis immunoglobulin M (IgM), FSH, LH, prolactin; progesterone; testosterone among infertile males. Standard world health organization protocol used to perform semen analysis.Results: A significant correlation between age group and CMV - IgM, HSV2 - IgM positivity (p=0.05 and 0.024). Type of infertility inversely correlated with T. gondii - IgM positivity (p=0.039) and positively correlated with Rubella - IgM (p=0.021). Significant correlation of T. gondii - CMV co-infection (p=0.048); T. gondii - Rubella co-infection (p=0.007); T. gondii - C. trachomatis co-infection (p=0.001). Significant correlation between semen parameters and TORCH positivity testosterone level significantly correlated with T. gondii - IgM; Rubella - IgM. Inverse significant correlation between C. trachomatis - IgM and FSH level. Significant correlations between TORCH - IgM positivity, semen parameters and hormones were following T. gondii, CMV, total sperm count and LH. T. gondii, Rubella, active motility and LH. T. gondii, total sperm count and prolactin. CMV, HSV2, C. trachomatis trachomatis, active sperm motility and progesterone. Rubella, sluggish motility and LH level. T. gondii; Rubella, sperm dead % and LH. T. gondii; sluggish motility and FSH. T. gondii; CMV; sluggish motility and FSH. T. gondii, sluggish motility and prolactin.HSV2, sluggish motility and progesterone level. T. gondii, CMV, HSV2, sperm dead % and progesterone. Sluggish motility, T. gondii, C. trachomatis, HSV2 and testosterone. T. gondii, C. trachomatis, sperm dead % and testosterone. C. trachomatis, HSV2; normal sperm morphology and LH. T. gondii, normal sperm morphology and prolactin level. HSV2, normal sperm morphology and testosterone. T. gondii, abnormal sperm morphology and prolactin. C. trachomatis, abnormal sperm morphology and LH.Conclusions: A current study proved several important and outstanding results about the role of TORCH agent and C. trachomatis in male infertility. TORCH agent and C. trachomatis have direct effects on sperm parameters. T. gondii and Rubella have a direct effect on testosterone levels; C. trachomatis has direct effect on FSH among infertile males. TORCH agent plays a
DOI: 10.6084/m9.figshare.6743348.v1
title: Intervention stuyd_primary care_educational outreach vistis for knee osteoarthritis

description: This file contains the original dataset to reproduce the outcomes