DOI: 10.5281/zenodo.1174376
title: Data - Effect Of Electrolytes As Adjuvants In Gfp And Lps Partitioning On Aqueous Two-Phase Systems: 1. Polymer-Polymer Systems

description: <strong>Overview</strong>

The production of recombinant biopharmaceuticals is highly dependent of a proper choice of the downstream processing stages. Particularly, the purification that must ensure that all the endotoxins (lipopolysaccharide - LPS) are efficiently removed from the final product. This dataset contains the raw data and statistical analysis for the research entitled - "Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems". 

<strong>Info</strong>

ANOVA_Turkey_Sub.R &lt;- code for ANOVA analysis in R statistic 3.3.3    <br>
glm.R &lt;- code for GLM analysis in R statistic 3.3.3<br>
K&REC_LPS_PEG_NaPA.xlsx &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) and recover (REC) for ANOVA analysis<br>
K&REC_LPS_PEG_NaPA_K.docx &lt;- File with ANOVA result of partition coefficient (K) for GFP<br>
K&REC_LPS_PEG_NaPA_REC.docx &lt;- File with ANOVA result of recover (REC) for GFP         <br>
K_GFP_Pol_005.csv &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.05M salt assays    <br>
K_GFP_Pol_005.doc &lt;- File with GLM analysis of GFP partition coefficient (K) in 0.05M salt assays  <br>
K_GFP_Pol_005_QQ.png &lt;- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.05M salt assays  <br>
K_GFP_Pol_025.csv &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays  <br>
K_GFP_Pol_025.doc  &lt;- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays  <br>
K_GFP_Pol_025_QQ.png &lt;- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.25M salt assays         <br>
REC_GFP_Pol_005.csv &lt;- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.05M salt assays         <br>
REC_GFP_Pol_005.doc  &lt;- File with GLM analysis of GFP recover (REC) in 0.05M salt assays    <br>
REC_GFP_Pol_005_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.05M salt assays   <br>
REC_GFP_Pol_025.csv &lt;- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.25M salt assays          <br>
REC_GFP_Pol_025.doc  &lt;- File with GLM analysis of GFP recover (REC) in 0.25M salt assays <br>
REC_GFP_Pol_025_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays        <br>
REM_LPS_PEG_NaPA.docx  &lt;- File with ANOVA result of LPS removal          <br>
REM_LPS_PEG_NaPA.xlsx &lt;- File with raw values organized in a spreadsheet of LPS removal for ANOVA analysis<br>
Stability_GFP_PEG_NaPA.docx &lt;- File with ANOVA result of GFP stability<br>
Stability_GFP_PEG_NaPA.xlsx &lt;- File with raw values organized in a spreadsheet of GFP stability results for ANOVA analysis

REM_LPS_Pol_005.csv  &lt;- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.05M salt assays         <br>
REM_LPS_Pol_005.doc  &lt;- File with GLM analysis of LPS removal (REM) in 0.05M salt assays    <br>
REM_LPS_Pol_005_QQ.png &lt;- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.05M salt assays  <br>
REM_LPS_Pol_025.csv  &lt;- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.25M salt assays         <br>
REM_LPS_Pol_025.doc   &lt;- File with GLM analysis of LPS removal (REM) in 0.25M salt assays    <br>
REM_LPS_Pol_025_QQ.png &lt;- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays

K_GFP_Pol_025_NaCl_Li2SO4.csv &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays comparing NaCl and Li2SO4 effect <br>
K_GFP_Pol_025_NaCl_Li2SO4.doc  &lt;- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays comparing NaCl and Li2SO4 effect <br>
K_GFP_Pol_025_NaCl_Li2SO4_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays comparing NaCl and Li2SO4 effect   

REM_LPS_Pol_KI_0.05_vs_0.25.csv &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KI assays comparing salt concentration effect  <br>
REM_LPS_Pol_KI_0.05_vs_0.25.doc &lt;- File with GLM analysis of GFP partition coefficient (K) in KI assays comparing salt concentration effect<br>
REM_LPS_Pol_KI_0.05_vs_0.25_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in KI assays comparing salt concentration effect<br>
REM_LPS_Pol_KNO3_0.05_vs_0.25.csv  &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KNO3 assays comparing salt concentration effect  <br>
REM_LPS_Pol_KNO3_0.05_vs_0.25.doc &lt;- File with GLM analysis of GFP partition coefficient (K) in KNO3 assays comparing salt concentration effect<br>
REM_LPS_Pol_KNO3_0.05_vs_0.25_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in KNO3 assays comparing salt concentration effect<br>
REM_LPS_Pol_Li2SO4_0.05_vs_0.25.csv &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in Li2SO4 assays comparing salt concentration effect  <br>
REM_LPS_Pol_Li2SO4_0.05_vs_0.25.doc &lt;- File with GLM analysis of GFP partition coefficient (K) in Li2SO4 assays comparing salt concentration effect<br>
REM_LPS_Pol_Li2SO4_0.05_vs_0.25_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in Li2SO4 assays comparing salt concentration effect<br>
REM_LPS_Pol_NaCl_0.05_vs_0.25.csv &lt;- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in NaCl assays comparing salt concentration effect  <br>
REM_LPS_Pol_NaCl_0.05_vs_0.25.doc &lt;- File with GLM analysis of GFP partition coefficient (K) in NaCl assays comparing salt concentration effect <br>
REM_LPS_Pol_NaCl_0.05_vs_0.25_QQ.png &lt;- Residual quantile plot of GLM analysis of GFP recover (REC) in NaCl assays comparing salt concentration effect

 

<strong>Annotation</strong>

12/12 - Concentration of 12% of each polymer PEG/NaPA

16/16 - Concentration of 16% of each polymer PEG/NaPA

P/N -  PEG/NaPA

10e4, 10e5, 10e6 - Concentration of LPS in scientific notation - 10000, 100000, 100000 EU/mL

poly - Polymer

salt - Salt concentration in the assay

tsalt - Type of salt in the assay (NaCl, KNO3, KI and Li2SO4)

lps - lipopolysaccharide

K - GFP partition coefficient

REM - LPS removal

REC - GFP recover

wo_salt - Assay without salt addition

<strong>Acknowledgements</strong>

The authors are grateful for financial support from FAPESP (São Paulo Research Foundation, Brazil) through the following projects: 2005/60159-7; 2007/51978-0; 2014/16424-7; and 2014/19793-3. The authors also acknowledge the support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) through the process #0366/09-9 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).

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