cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1160489.v1
title: Development, morphological characteristics and viability of temporary cysts of <i>Pyrodinium bahamense</i> var. <i>compressum</i> (Dinophyceae) <i>in vitro</i>

description: Pellicle or temporary cysts of <i>Pyrodinium bahamense</i> var. <i>compressum</i> (Böhm) Steidinger, Tester & F.J.R. Taylor and their role in bloom dynamics have not yet been adequately characterized and understood. We investigated the role of temperature- and nutrient-mediated stress as factors that could induce pellicle formation in batch cultures. Cellular features and their implications for temporary cyst viability were examined using confocal laser scanning microscopy (CLSM). Our data suggest that temperature change is one of the key factors influencing pellicle formation, preserving viability at low temperature (i.e. 13°C). Hypnocysts (resting cysts) were not observed. During pellicle formation, motile cells generally undergo ecdysis, extrusion of cytoplasmic materials and bacteria, compaction of the nucleus and non-motility. The outermost covering of the temporary cysts shows red autofluorescence and it contains lower concentrations of chlorophyll (chl) <i>a</i> and no detectable chl <i>c.</i> The nuclear region is surrounded by transitional red bodies and other unidentified cellular structures. Temporary cysts can immediately revert back to the motile state upon exposure to optimum conditions. This is accompanied by the expansion of the nuclear region, regeneration of the chloroplasts and enlargement of the cell. Developmental changes during reversal of temporary cysts to motile forms were also observed to cause breaks in the cell covering that could serve as sites for bacterial entry. Though observed <i>in vitro</i>, such behaviour may also be occurring in nature especially as a response to drastic short-lived environmental changes. This is the first detailed report on the characteristics of temporary cysts of <i>P. bahamense</i> var<i>. compressum.</i>
DOI: 10.6084/m9.figshare.1160499.v5
title: Project files for a passively cooled, low-cost, low tech medicine warehouse

description: This dataset is constituted by two types of files that have supported the definition of a design hypothesis for a passively-cooled, low-cost, low-tech medicine warehouse for hot climates. The first is a Sketchup file of the architectural model. Its layers are conceived to be turned on and off to explore the construction phases and details. The second file type is constituted by ESP-r model files (created with version 11 for Cygwin) ordered in directories. Each directory is named after a climatic location. The "cfg" folder of each directory contains several model configurations files (".cfg"). The project to which the above files refer to and the considerations deriving from the thermal simulations are presented in the publication: Gian Luca Brunetti (2009). Guidelines for the Design and Construction of Passively-cooled, Low-cost, Low-tech Medicine Warehouses for Hot Climates, and a Design Hypothesis Based on Dry Downdraught Cooling. Gian Luca Brunetti, Department of Architecture and Urban Studies (DAStU), Polytechnic University of Milan. gianluca.brunetti@polimi.it.
DOI: 10.5517/ccdc.csd.cc2368l6
title: CCDC 1942447: Experimental Crystal Structure Determination

description: Related Article: Yuqi Tan, Feng Han, Marcel Hemming, Jie Wang, Klaus Harms, Xiulan Xie, Eric Meggers|2020|Org.Lett.|22|6653|doi:10.1021/acs.orglett.0c02452
DOI: 10.6084/m9.figshare.1160487
title: Molecular diversity of green corticolous microalgae from two sub-Mediterranean European localities

description: Green algae in corticolous biofilms are simple coccoid cells or filamentous thalli with strikingly low morphological diversity. Consequently, microscopic identification of these organisms is difficult, and often possible only to higher taxonomic units. We investigated the taxonomic and phylogenetic composition of green microalgae isolated from biofilms growing on the bark of <i>Quercus pubescens</i> and <i>Pinus nigra</i>. The study was based on 122 partial sequences of the plastid-encoded <i>rbc</i>L gene. In total, 29 operational taxonomic units (OTUs), differing in their <i>rbc</i>L sequences, were encountered. Members of the Trebouxiophyceae formed 97.5% of the isolates; Streptophyta made up 2.5%. The most frequently occurring OTUs were in the genera <i>Coccomyxa, Parachloroidium</i> and <i>Stichococcus</i>. Within the <i>Watanabea</i> clade, we have probably discovered an as-yet undescribed generic lineage with chlorelloid morphology. OTUs belonging to the recently described trebouxiophycean genera <i>Kalinella, Leptochlorella</i> and <i>Xylochloris</i> were also encountered, which indicates that these genera are probably widely distributed in subaerial microhabitats, such as tree bark. The samples taken from oak trees were more diverse in their OTU composition than those taken from pine trees, but the average phylogenetic distances of OTUs in samples did not differ between the host tree taxa. Host tree species had a stronger effect on the community structure of algae than the sampling locality. This indicates that habitat filtering is important for the distribution of individual microalgal phylogenetic taxa.
DOI: 10.6084/m9.figshare.1160488
title: Symmetry and sex in Bacillariaceae (Bacillariophyta), with descriptions of three new <i>Nitzschia</i> species

description: The large diatom genus <i>Nitzschia</i> and the morphologically similar <i>Hantzschia</i> are currently distinguished on the basis of cell symmetry and division. In <i>Hantzschia</i>, the eccentrically placed raphe systems of the two valves are always on the same side of the frustule (‘hantzschioid’ symmetry), never diagonally opposite (‘nitzschioid’ symmetry). Although some <i>Nitzschia</i> species produce hantzschioid cells, it has previously been thought that these cells never breed true but divide to give one nitzschioid daughter and one hantzschioid daughter. Sublittoral marine epipelon from Loch Goil, W Scotland, contained three undescribed species of Bacillariaceae whose cells were always hantzschioid, but which possessed a valve structure (silica flaps on either side of the raphe, no central raphe endings, a slit-like entrance to the raphe canal that narrows towards the poles, and discrete bar-like fibulae) linking them to <i>N. spathulata</i> and the type species of <i>Nitzschia, N. sigmoidea</i>. They also agreed with these species in chloroplast morphology and auxosporulation and are therefore assigned to <i>Nitzschia</i> as <i>N. dicrogramma, N. brachygramma</i> and <i>N. parkii, spp. nov</i>. Consequently the only type of cell division found in all <i>Hantzschia</i> species (in which a hantzschioid parent cell divides to give two hantzschioid daughters) is not unique to <i>Hantzschia</i> but also occurs in at least one lineage of <i>Nitzschia</i>. ‘Core’ <i>Hantzschia</i> species may nevertheless comprise a monophyletic group, characterized by an unusual kind of internal central raphe endings. Four symmetry categories are now known in <i>Hantzschia</i> and <i>Nitzschia</i>: (1) all cells hantzschioid; (2) hantzschioid and nitzschioid cells in a 2 : 1 ratio; (3) hantzschioid and nitzschioid cells in a 1 : 1 ratio; and (4) all cells nitzschioid. The division pattern seems to be constant within species and may therefore be useful as a taxonomic character above the species level. <i>Hantzschia segmentalis</i> is given its first written description.
DOI: 10.5517/ccdc.csd.cc23dlsx
title: CCDC 1948529: Experimental Crystal Structure Determination

description: Related Article: Tapan Ghosh, Srikanth Birudula, Kalyan Jyoti Kalita, Ratheesh K. Vijayaraghavan|2020|Chem.-Eur.J.|26|10501|doi:10.1002/chem.202000061
DOI: 10.6084/m9.figshare.1160487.v1
title: Molecular diversity of green corticolous microalgae from two sub-Mediterranean European localities

description: Green algae in corticolous biofilms are simple coccoid cells or filamentous thalli with strikingly low morphological diversity. Consequently, microscopic identification of these organisms is difficult, and often possible only to higher taxonomic units. We investigated the taxonomic and phylogenetic composition of green microalgae isolated from biofilms growing on the bark of <i>Quercus pubescens</i> and <i>Pinus nigra</i>. The study was based on 122 partial sequences of the plastid-encoded <i>rbc</i>L gene. In total, 29 operational taxonomic units (OTUs), differing in their <i>rbc</i>L sequences, were encountered. Members of the Trebouxiophyceae formed 97.5% of the isolates; Streptophyta made up 2.5%. The most frequently occurring OTUs were in the genera <i>Coccomyxa, Parachloroidium</i> and <i>Stichococcus</i>. Within the <i>Watanabea</i> clade, we have probably discovered an as-yet undescribed generic lineage with chlorelloid morphology. OTUs belonging to the recently described trebouxiophycean genera <i>Kalinella, Leptochlorella</i> and <i>Xylochloris</i> were also encountered, which indicates that these genera are probably widely distributed in subaerial microhabitats, such as tree bark. The samples taken from oak trees were more diverse in their OTU composition than those taken from pine trees, but the average phylogenetic distances of OTUs in samples did not differ between the host tree taxa. Host tree species had a stronger effect on the community structure of algae than the sampling locality. This indicates that habitat filtering is important for the distribution of individual microalgal phylogenetic taxa.
DOI: 10.5517/ccdc.csd.cc23dlty
title: CCDC 1948530: Experimental Crystal Structure Determination

description: Related Article: Tapan Ghosh, Srikanth Birudula, Kalyan Jyoti Kalita, Ratheesh K. Vijayaraghavan|2020|Chem.-Eur.J.|26|10501|doi:10.1002/chem.202000061
DOI: 10.6084/m9.figshare.1160488.v1
title: Symmetry and sex in Bacillariaceae (Bacillariophyta), with descriptions of three new <i>Nitzschia</i> species

description: The large diatom genus <i>Nitzschia</i> and the morphologically similar <i>Hantzschia</i> are currently distinguished on the basis of cell symmetry and division. In <i>Hantzschia</i>, the eccentrically placed raphe systems of the two valves are always on the same side of the frustule (‘hantzschioid’ symmetry), never diagonally opposite (‘nitzschioid’ symmetry). Although some <i>Nitzschia</i> species produce hantzschioid cells, it has previously been thought that these cells never breed true but divide to give one nitzschioid daughter and one hantzschioid daughter. Sublittoral marine epipelon from Loch Goil, W Scotland, contained three undescribed species of Bacillariaceae whose cells were always hantzschioid, but which possessed a valve structure (silica flaps on either side of the raphe, no central raphe endings, a slit-like entrance to the raphe canal that narrows towards the poles, and discrete bar-like fibulae) linking them to <i>N. spathulata</i> and the type species of <i>Nitzschia, N. sigmoidea</i>. They also agreed with these species in chloroplast morphology and auxosporulation and are therefore assigned to <i>Nitzschia</i> as <i>N. dicrogramma, N. brachygramma</i> and <i>N. parkii, spp. nov</i>. Consequently the only type of cell division found in all <i>Hantzschia</i> species (in which a hantzschioid parent cell divides to give two hantzschioid daughters) is not unique to <i>Hantzschia</i> but also occurs in at least one lineage of <i>Nitzschia</i>. ‘Core’ <i>Hantzschia</i> species may nevertheless comprise a monophyletic group, characterized by an unusual kind of internal central raphe endings. Four symmetry categories are now known in <i>Hantzschia</i> and <i>Nitzschia</i>: (1) all cells hantzschioid; (2) hantzschioid and nitzschioid cells in a 2 : 1 ratio; (3) hantzschioid and nitzschioid cells in a 1 : 1 ratio; and (4) all cells nitzschioid. The division pattern seems to be constant within species and may therefore be useful as a taxonomic character above the species level. <i>Hantzschia segmentalis</i> is given its first written description.
DOI: 10.6084/m9.figshare.1160485
title: Herbivore-induced defence response in the brown seaweed <i>Fucus vesiculosus</i> (Phaeophyceae): temporal pattern and gene expression

description: This study aimed to reveal the dynamics of inducible anti-herbivory traits in the brown seaweed <i>Fucus vesiculosus</i>, in response to grazing by the isopod <i>Idotea baltica</i>. As the molecular basis of defence induction in seaweeds is poorly understood, a transcriptomic approach was used to gain insight into cellular processes underlying defence induction and thus promote the mechanistic understanding of anti-herbivory responses in seaweeds. In a 27 day feeding-assayed induction experiment, temporal patterns of the induced anti-herbivory resistance of <i>F. vesiculosus</i> were assessed under laboratory conditions. Feeding assays were performed at three day intervals, using fresh and reconstituted food. Microarray hybridizations investigating the expression of genes 3 days before, as well as at the same time as, feeding assays revealed a decreased palatability of previously grazed <i>F. vesiculosus</i> pieces compared with non-grazed control pieces. Despite permanent exposure to grazers, <i>F. vesiculosus</i> palatability varied over time. Non-grazed <i>F. vesiculosus</i> pieces were significantly preferred to grazed pieces after 18 and again after 27 days of previous grazing, while their relative palatability for isopods was comparable at all other times during the experiment. Relative to controls, 562/402 genes were ≥ 1.5-fold up-/down-regulated in seaweed pieces that were grazed for 18 days, i.e. when induction of defences was detected in feeding assays. Massive reprogramming of the regulatory expression orchestra (translation, transcription) as well as up-regulation of genes involved in lipid and carbohydrate metabolism, intracellular trafficking, defence and stress response was found. At the same time, down-regulation of photosynthesis was observed in grazed seaweed pieces. This study suggests a high level of temporal variability in induced anti-herbivory traits of <i>F. vesiculosus</i> and reveals increased expression of genes with putative defensive functions in conjunction with the reallocation of resources from primary to secondary metabolism.
DOI: 10.6084/m9.figshare.1160485.v1
title: Herbivore-induced defence response in the brown seaweed <i>Fucus vesiculosus</i> (Phaeophyceae): temporal pattern and gene expression

description: This study aimed to reveal the dynamics of inducible anti-herbivory traits in the brown seaweed <i>Fucus vesiculosus</i>, in response to grazing by the isopod <i>Idotea baltica</i>. As the molecular basis of defence induction in seaweeds is poorly understood, a transcriptomic approach was used to gain insight into cellular processes underlying defence induction and thus promote the mechanistic understanding of anti-herbivory responses in seaweeds. In a 27 day feeding-assayed induction experiment, temporal patterns of the induced anti-herbivory resistance of <i>F. vesiculosus</i> were assessed under laboratory conditions. Feeding assays were performed at three day intervals, using fresh and reconstituted food. Microarray hybridizations investigating the expression of genes 3 days before, as well as at the same time as, feeding assays revealed a decreased palatability of previously grazed <i>F. vesiculosus</i> pieces compared with non-grazed control pieces. Despite permanent exposure to grazers, <i>F. vesiculosus</i> palatability varied over time. Non-grazed <i>F. vesiculosus</i> pieces were significantly preferred to grazed pieces after 18 and again after 27 days of previous grazing, while their relative palatability for isopods was comparable at all other times during the experiment. Relative to controls, 562/402 genes were ≥ 1.5-fold up-/down-regulated in seaweed pieces that were grazed for 18 days, i.e. when induction of defences was detected in feeding assays. Massive reprogramming of the regulatory expression orchestra (translation, transcription) as well as up-regulation of genes involved in lipid and carbohydrate metabolism, intracellular trafficking, defence and stress response was found. At the same time, down-regulation of photosynthesis was observed in grazed seaweed pieces. This study suggests a high level of temporal variability in induced anti-herbivory traits of <i>F. vesiculosus</i> and reveals increased expression of genes with putative defensive functions in conjunction with the reallocation of resources from primary to secondary metabolism.
DOI: 10.5517/ccdc.csd.cc240ryw
title: CCDC 1966948: Experimental Crystal Structure Determination

description: Related Article: Itai Massad, Heiko Sommer, Ilan Marek|2020|Angew.Chem.,Int.Ed.|59|15549|doi:10.1002/anie.202005058
DOI: 10.5517/ccdc.csd.cc2488qf
title: CCDC 1974164: Experimental Crystal Structure Determination

description: Related Article: Wen Zhang, Xiangtao Chen, Yi An, Jiaqi Wang, Chunlin Zhuang, Pei Tang, Fener Chen|2020|Chem.-Eur.J.|26|10439|doi:10.1002/chem.202002404
DOI: 10.6084/m9.figshare.1160428
title: Studying the structure of single-component ceramide bilayers with molecular dynamics simulations using different force fields

description: Molecular dynamics simulations are performed on a lipid bilayer that consists of ceramide NS 24:0 in an attempt to examine several structural and physicochemical properties of the specific system. The simulations are carried out with five different force fields (OPLS, GROMOS, BERGER, CHARMM and GAFF) in order to evaluate and compare their performance in modelling lipid systems that contain ceramides. The examined properties include bilayer thickness, chain tilt, density profiles, order parameters, chain conformation, area per lipid and (intermolecular or intramolecular) hydrogen bonding between the head groups. Special focus is given to the lateral lipid arrangement. To this purpose, a method is proposed that utilises the radial distribution functions of the alkyl chains to derive quantitative information about the lateral lipid packing. In most cases, all force fields lead to similar results. For a few properties (e.g. intramolecular hydrogen bonding), there is some discrepancy between the force fields but the lack of respective experimental data does not allow an unambiguous conclusion on which force field is the most reliable.
DOI: 10.6084/m9.figshare.1160428.v2
title: Studying the structure of single-component ceramide bilayers with molecular dynamics simulations using different force fields

description: Molecular dynamics simulations are performed on a lipid bilayer that consists of ceramide NS 24:0 in an attempt to examine several structural and physicochemical properties of the specific system. The simulations are carried out with five different force fields (OPLS, GROMOS, BERGER, CHARMM and GAFF) in order to evaluate and compare their performance in modelling lipid systems that contain ceramides. The examined properties include bilayer thickness, chain tilt, density profiles, order parameters, chain conformation, area per lipid and (intermolecular or intramolecular) hydrogen bonding between the head groups. Special focus is given to the lateral lipid arrangement. To this purpose, a method is proposed that utilises the radial distribution functions of the alkyl chains to derive quantitative information about the lateral lipid packing. In most cases, all force fields lead to similar results. For a few properties (e.g. intramolecular hydrogen bonding), there is some discrepancy between the force fields but the lack of respective experimental data does not allow an unambiguous conclusion on which force field is the most reliable.
DOI: 10.6084/m9.figshare.1160428.v1
title: Studying the structure of single-component ceramide bilayers with molecular dynamics simulations using different force fields

description: Molecular dynamics simulations are performed on a lipid bilayer that consists of ceramide NS 24:0 in an attempt to examine several structural and physicochemical properties of the specific system. The simulations are carried out with five different force fields (OPLS, GROMOS, BERGER, CHARMM and GAFF) in order to evaluate and compare their performance in modelling lipid systems that contain ceramides. The examined properties include bilayer thickness, chain tilt, density profiles, order parameters, chain conformation, area per lipid and (intermolecular or intramolecular) hydrogen bonding between the head groups. Special focus is given to the lateral lipid arrangement. To this purpose, a method is proposed that utilises the radial distribution functions of the alkyl chains to derive quantitative information about the lateral lipid packing. In most cases, all force fields lead to similar results. For a few properties (e.g. intramolecular hydrogen bonding), there is some discrepancy between the force fields but the lack of respective experimental data does not allow an unambiguous conclusion on which force field is the most reliable.
DOI: 10.6084/m9.figshare.1159900
title: Emotion Science Symposium

description: Presnted at the 2014 New Zealand Psychological Society conference in Nelson, NZ. <br>Presentations: Smiling to Smiles After Exclusion: Social Rejection Enhances Affiliative Signalling. presenter: Michael Philipp, Massey University. Emoticons as genuine expressions of emotion. presenter: Tracey Bear, Massey University. Do Hypoxia-driven Changes in Mood Affect Cognition? presenter: Stephen Hill, Massey University. Predictably regretful: A comparison of the effects of time, domain, justification, and life rule contradiction on the intensity of regrets. presenter: Matt Williams, Massey University. The effect of gender and cognitive errors on depressive symptoms in adolescence. presenter: Tatiana Tairi, Massey University.
DOI: 10.6084/m9.figshare.1159370
title: SBNeC2014 Posters

description: Posters from LaNeC (Laboratório de Neurociências e Comportamento "Miguel Ozório de Almeida") presented at SBnEC 2014 (http://sbnec.org.br/congresso2014/)
DOI: 10.6084/m9.figshare.1159900.v1
title: Emotion Science Symposium

description: Presnted at the 2014 New Zealand Psychological Society conference in Nelson, NZ. <br>Presentations: Smiling to Smiles After Exclusion: Social Rejection Enhances Affiliative Signalling. presenter: Michael Philipp, Massey University. Emoticons as genuine expressions of emotion. presenter: Tracey Bear, Massey University. Do Hypoxia-driven Changes in Mood Affect Cognition? presenter: Stephen Hill, Massey University. Predictably regretful: A comparison of the effects of time, domain, justification, and life rule contradiction on the intensity of regrets. presenter: Matt Williams, Massey University. The effect of gender and cognitive errors on depressive symptoms in adolescence. presenter: Tatiana Tairi, Massey University.
DOI: 10.6084/m9.figshare.1159900.v2
title: Emotion Science Symposium

description: Presnted at the 2014 New Zealand Psychological Society conference in Nelson, NZ. <br>Presentations: Smiling to Smiles After Exclusion: Social Rejection Enhances Affiliative Signalling. presenter: Michael Philipp, Massey University. Emoticons as genuine expressions of emotion. presenter: Tracey Bear, Massey University. Do Hypoxia-driven Changes in Mood Affect Cognition? presenter: Stephen Hill, Massey University. Predictably regretful: A comparison of the effects of time, domain, justification, and life rule contradiction on the intensity of regrets. presenter: Matt Williams, Massey University. The effect of gender and cognitive errors on depressive symptoms in adolescence. presenter: Tatiana Tairi, Massey University.