cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7506317.v1
title: HEMATOLOGICAL PROFILE OF SANTA INÊS EWES SUPPLEMENTED ON PASTURE AT THE LAST THIRD OF PREGNANCY AND POSTPARTUM

description: AbstractThe purpose of this study was to evaluate the influence of concentrate supplementation level on hematological profile of Santa Ines ewes, at the last third of pregnancy and postpartum, and of their lambs. We used eleven ewes at the last 75 days of pregnancy and the first 75 days of lactation and eight lambs born from these ewes, allotted in a completely randomized design and split-plot arrangement in time. Blood samples were collected every fourteen days. Neither the erythrocyte nor the leukocyte profile was affected by the different levels of supplementation (P&gt;0.05) and the blood components evaluated were within the reference range for adult sheep. The animal category affected (P&lt;0.05) erythrocytes, and the highest values (11.72 x 106/p.l) were observed in lambs. Lambs showed lower values (3073.3/µL) for neutrophils in comparison with ewes, during pregnancy (4957.6/µL) or postpartum (4067.3/µL); these values did not differ (P&gt;0.05). For the lymphocyte count, the values found in lambs (2858.8 /µL) were similar to those in pregnant ewes (2,982.0/µL); lactating ewes (4119.8/µL) showed the highest values. Concentrate supplementation (0.5% BW) at pre and postpartum does not alter the erythrocyte and leukocyte profile of Santa Inês sheep, with the largest differences between the two sheep categories and lambs.
DOI: 10.6084/m9.figshare.7515212
title: INCLUSION OF GUAVA WASTES IN THE DIET OF EUROPEAN QUAILS

description: Abstract:This experiment was conducted to analyze the performance and carcass characteristics of European quail fed agroindustry residue of guava in substitution of corn. 140 birds were used, distributed in a completely randomized design. The treatments consisted of a control diet and four diets with levels of guava waste inclusion (2.5, 5.0, 7.5 and 10.0%) to a diet based on corn and soybean meal. There were no significant differences (P&gt; 0.05) for the variables: weight gain, feed intake, feed conversion, feed efficiency, weight and carcass yield and prime cuts (breast, drumstick and thigh) wings, back, neck, head, feet and foodstuffs organs (heart, liver and gizzard). The guava waste can be used as alternative ingredient in the diets of European quail in the period of 16-38 days of age, up to the level of 10% inclusion without depressing the performance and yield of poultry carcasses
DOI: 10.6084/m9.figshare.7515212.v1
title: INCLUSION OF GUAVA WASTES IN THE DIET OF EUROPEAN QUAILS

description: Abstract:This experiment was conducted to analyze the performance and carcass characteristics of European quail fed agroindustry residue of guava in substitution of corn. 140 birds were used, distributed in a completely randomized design. The treatments consisted of a control diet and four diets with levels of guava waste inclusion (2.5, 5.0, 7.5 and 10.0%) to a diet based on corn and soybean meal. There were no significant differences (P&gt; 0.05) for the variables: weight gain, feed intake, feed conversion, feed efficiency, weight and carcass yield and prime cuts (breast, drumstick and thigh) wings, back, neck, head, feet and foodstuffs organs (heart, liver and gizzard). The guava waste can be used as alternative ingredient in the diets of European quail in the period of 16-38 days of age, up to the level of 10% inclusion without depressing the performance and yield of poultry carcasses
DOI: 10.6084/m9.figshare.7508237
title: PRODUCTION OF IN VITRO EMBRYO USING SEXED SPERM OF 5/8 GIROLANDO BULLS

description: AbstractWe evaluated the "in vitro" blastocyst rate production using bovine sexed semen. Semen from three bulls was used to verify the individual's semen variation, cleavage rates and embryo production. In this study, we employed reproductive biotechnologies, computer analysis of post-thawed semen and fluorescent probes for sperm cells integrity analysis (plasma membrane, acrosome membrane and mitochondrial potential). A total of 959 oocysts went through in vitro maturation steps for in vitro fertilization and cultivation, being 473 with sexed semen and 486 with conventional semen. The cleavage rate was observed in blastocysts on D2 and D7. Data were analyzed by SPSS 16.0 software using analysis of variance (ANOVA), Student's t-test was used to detect differences between groups, and chi-square analysis for in vitro production results (P &lt;0.05 ). The results differed between conventional (31.06%) and sexed semen (21.10%) in the obtainment of blastocyst. When the blastocyst production was individually compared in sexed semen samples (27.69%, 17.93% and 25.56%, bulls 1, 2 and 3, respectively) we verified T2
DOI: 10.6084/m9.figshare.7508237.v1
title: PRODUCTION OF IN VITRO EMBRYO USING SEXED SPERM OF 5/8 GIROLANDO BULLS

description: AbstractWe evaluated the "in vitro" blastocyst rate production using bovine sexed semen. Semen from three bulls was used to verify the individual's semen variation, cleavage rates and embryo production. In this study, we employed reproductive biotechnologies, computer analysis of post-thawed semen and fluorescent probes for sperm cells integrity analysis (plasma membrane, acrosome membrane and mitochondrial potential). A total of 959 oocysts went through in vitro maturation steps for in vitro fertilization and cultivation, being 473 with sexed semen and 486 with conventional semen. The cleavage rate was observed in blastocysts on D2 and D7. Data were analyzed by SPSS 16.0 software using analysis of variance (ANOVA), Student's t-test was used to detect differences between groups, and chi-square analysis for in vitro production results (P &lt;0.05 ). The results differed between conventional (31.06%) and sexed semen (21.10%) in the obtainment of blastocyst. When the blastocyst production was individually compared in sexed semen samples (27.69%, 17.93% and 25.56%, bulls 1, 2 and 3, respectively) we verified T2
DOI: 10.6084/m9.figshare.7512701
title: CREATINE PHOSPHOKINASE ISOENZYME-MB MASS (CK-MB MASS) AND TROPONIN I (cTnI) IN DOGS (Canis familiaris)

description: AbstractCardiac markers, especially CK-MB mass and cTnI, have an essential role in both human and veterinary clinical and surgical cardiology, allowing a more precise and accurate diagnosis in myocardial lesions. The goal of this work was to measure these cardiac markers in veterinary medicine, improve their use and provide information about these laboratory methods that allow non-invasive health monitoring of the myocardial cell. Parameters quantification was obtained from sera of healthy dogs examined during routine procedures at the Small Animal Veterinary Hospital of Universidade Estadual do Norte Fluminense Darcy Ribeiro. The human chemiluminescence essay turbo kit (IMMULITE Turbo, Siemens ®) test proved to be effective in canine species for both CK-MB mass and cTnI. In addition, the values found for CK-MB will significantly contribute to clinical routine or experimental work with dogs.
DOI: 10.6084/m9.figshare.7512701.v1
title: CREATINE PHOSPHOKINASE ISOENZYME-MB MASS (CK-MB MASS) AND TROPONIN I (cTnI) IN DOGS (Canis familiaris)

description: AbstractCardiac markers, especially CK-MB mass and cTnI, have an essential role in both human and veterinary clinical and surgical cardiology, allowing a more precise and accurate diagnosis in myocardial lesions. The goal of this work was to measure these cardiac markers in veterinary medicine, improve their use and provide information about these laboratory methods that allow non-invasive health monitoring of the myocardial cell. Parameters quantification was obtained from sera of healthy dogs examined during routine procedures at the Small Animal Veterinary Hospital of Universidade Estadual do Norte Fluminense Darcy Ribeiro. The human chemiluminescence essay turbo kit (IMMULITE Turbo, Siemens ®) test proved to be effective in canine species for both CK-MB mass and cTnI. In addition, the values found for CK-MB will significantly contribute to clinical routine or experimental work with dogs.
DOI: 10.6084/m9.figshare.7519241
title: SEROLOGICAL EVIDENCE OF EXPOSURE TO Anaplasma phagocytophilum IN HORSES FROM THE RIO DE JANEIRO STATE MOUNTED POLICE BRED IN THE URBAN ZONE

description: AbstractAnaplasma phagocytophilum is an emergent hemoparasite in regions where ticks are regularly found. In order to investigate the rate of anti-A. phagocytophilum antibody in horses of the Mounted Police of Rio de Janeiro state and therefore the presence of this agent, 41 horses from the Cavalry Squadron (CS) and 50 from the Regiment of Mounted Police (RMP) were selected. For the serologic diagnosis the Indirect Fluorescent Antibody test was performed. In the present work, among 91 equine sera samples anti-A. phagocytophilum antibodies, with titer ≥ 1:80, were detected in 11 animals (12%), being five (5%) from males and six (7%) females, with no statistical difference. The adult animals with ages varying between five and 14 years presented the highest rate of positive reaction, although antibodies were detected in animals of all ages. We did not observe statistic differences in relation to the presence of anti-A. phagocytophilum antibodies among the animals bred in the CS and RMP. The presence of anti-A.phagocytophilim antibodies in horses of the Mounted Police with no clinical signs is indicative that the parasite is present in the enzootic form among the horses of the urban area. The circulation of the parasite among the animals is not dependent of the presence of tick infestations.
DOI: 10.6084/m9.figshare.7519241.v1
title: SEROLOGICAL EVIDENCE OF EXPOSURE TO Anaplasma phagocytophilum IN HORSES FROM THE RIO DE JANEIRO STATE MOUNTED POLICE BRED IN THE URBAN ZONE

description: AbstractAnaplasma phagocytophilum is an emergent hemoparasite in regions where ticks are regularly found. In order to investigate the rate of anti-A. phagocytophilum antibody in horses of the Mounted Police of Rio de Janeiro state and therefore the presence of this agent, 41 horses from the Cavalry Squadron (CS) and 50 from the Regiment of Mounted Police (RMP) were selected. For the serologic diagnosis the Indirect Fluorescent Antibody test was performed. In the present work, among 91 equine sera samples anti-A. phagocytophilum antibodies, with titer ≥ 1:80, were detected in 11 animals (12%), being five (5%) from males and six (7%) females, with no statistical difference. The adult animals with ages varying between five and 14 years presented the highest rate of positive reaction, although antibodies were detected in animals of all ages. We did not observe statistic differences in relation to the presence of anti-A. phagocytophilum antibodies among the animals bred in the CS and RMP. The presence of anti-A.phagocytophilim antibodies in horses of the Mounted Police with no clinical signs is indicative that the parasite is present in the enzootic form among the horses of the urban area. The circulation of the parasite among the animals is not dependent of the presence of tick infestations.
DOI: 10.6084/m9.figshare.7506479
title: OCCURRENCE OF ANTIBODIES AGAINST EQUINE HERPESVIRUS AND EQUINE ARTERITIS VIRUS IN HORSE HERDS OF RIO DE JANEIRO STATE, BRAZIL

description: AbstractInfections by equine herpesvirus (EHV) and equine arteritis virus (EAV) have been associated with important economic losses for the equine industry worldwide. Serological studies have indicated the presence of these agents in Brazil. The aim of this study was to evaluate the occurrence of specific antibodies against EAV and EHV in horse herds from different regions of Rio de Janeiro state. For this purpose, serum samples of 581 non-vaccinated animals and 44 breeding mares regularly vaccinated against equine rhinopneumonitis were collected. All samples were submitted to the virus neutralization test for the detection of specific antibodies to each virus. Results showed 29.6% (172/581) of seropositive animals for EHV (titers between 2 and ≥ 256) and 0.79% (5/630) for EAV (titers between 2 and 4,096). Considering the non-vaccinated animals, these findings demonstrated that specific antibodies were induced after natural exposure to the respective viruses, suggesting a probable circulation of these agents in the studied herds.
DOI: 10.6084/m9.figshare.7506479.v1
title: OCCURRENCE OF ANTIBODIES AGAINST EQUINE HERPESVIRUS AND EQUINE ARTERITIS VIRUS IN HORSE HERDS OF RIO DE JANEIRO STATE, BRAZIL

description: AbstractInfections by equine herpesvirus (EHV) and equine arteritis virus (EAV) have been associated with important economic losses for the equine industry worldwide. Serological studies have indicated the presence of these agents in Brazil. The aim of this study was to evaluate the occurrence of specific antibodies against EAV and EHV in horse herds from different regions of Rio de Janeiro state. For this purpose, serum samples of 581 non-vaccinated animals and 44 breeding mares regularly vaccinated against equine rhinopneumonitis were collected. All samples were submitted to the virus neutralization test for the detection of specific antibodies to each virus. Results showed 29.6% (172/581) of seropositive animals for EHV (titers between 2 and ≥ 256) and 0.79% (5/630) for EAV (titers between 2 and 4,096). Considering the non-vaccinated animals, these findings demonstrated that specific antibodies were induced after natural exposure to the respective viruses, suggesting a probable circulation of these agents in the studied herds.
DOI: 10.6084/m9.figshare.7519502
title: GESTATIONAL ECHO BIOMETRY IN BRACHYCEPHALIC PREGNANT BITCHES

description: AbstractUltrasonography is an accurate pregnancy diagnostic method, besides being completely innocuous for female and fetuses evaluation. The objective of this paper was to determine the reference values for gestational echo biometry of different breeds of bitches. A total of 25 multiparous females were included in the experiment, five English Bulldog bitches, five Pugs and 15 Shih Tzu, weighing 4-25 kg and aged 4-6 years. The echo biometric assessments were performed during the 2nd, 5th, 6th, 7th and 8th weeks of pregnancy, including gestational vesicle diameter, femur length, placenta thickness, parietal diameter, liver, heart and abdominal diameter and area. Early echo biometric study started at the second week of gestation. Measurements like fetal heart and liver diameter and area are still poorly studied, but can provide useful information for early detection of congenital anomalies that may reduce the viability of pregnancy. The significant results (P &lt; 0.001) obtained for biometrics (P &lt; 0.001) of the parietal (r<sup>2</sup> = 81%) and abdominal diameter (r<sup>2</sup> = 86%), abdominal area (r<sup>2</sup> = 80%), femur length (r<sup>2</sup> = 84%), cardiac length (r<sup>2</sup> = 79%), width (r<sup>2</sup> = 79%), area (r<sup>2</sup> = 82%) and volume (r<sup>2</sup>= 72%) and liver area (r<sup>2</sup> = 71%) in brachycephalic conceptus may help to assess the development of fetuses, complementing the conventional gestational ultrasound of bitches and become a model for the study in other breeds of dogs and alternative animal species.
DOI: 10.6084/m9.figshare.7513466.v1
title: PREVALENCE OF ARBOVIRUS ANTIBODIES AGAINST THE FAMILY Bunyaviridae IN WATER BUFFALOES

description: AbstractThe State of Pará comprises 26% of Brazilian Amazon region where a large diversity of arboviruses has been described. This study sought to assess the prevalence and distribution of haemagglutination-inhibition antibodies against antigens of nine different types of arbovirus of the Bunyaviridae family, where eight were Orthobunyavirus: Guaroa virus, Maguari virus, Tacaiuma virus, Utinga virus, Belem virus, Caraparu virus, Oropouche virus and Catu virus, and one Phlebovirus: Icoaraci virus in sera samples of water buffaloes in Pará State, Brazil. For all Arboviruses investigated there were antibodies, with the exception of Belem virus. Antibodies to Maguari viruswere more prevalent (7.33%). The water buffaloes of the present study showed variable levels of antibodies in monotypic and heterotypic reactions that may indicate there are movements from most bunyavirus studied in domestic buffaloes in the state of Pará, and the Maguari virus presents the largest circulation. Therefore, further studies are needed to investigate the role of water buffalo in the maintenance and dispersal of arboviruses, as well as whether these viruses can cause disease in that species, especially in cases of birth defects and abortions.
DOI: 10.6084/m9.figshare.7519502.v1
title: GESTATIONAL ECHO BIOMETRY IN BRACHYCEPHALIC PREGNANT BITCHES

description: AbstractUltrasonography is an accurate pregnancy diagnostic method, besides being completely innocuous for female and fetuses evaluation. The objective of this paper was to determine the reference values for gestational echo biometry of different breeds of bitches. A total of 25 multiparous females were included in the experiment, five English Bulldog bitches, five Pugs and 15 Shih Tzu, weighing 4-25 kg and aged 4-6 years. The echo biometric assessments were performed during the 2nd, 5th, 6th, 7th and 8th weeks of pregnancy, including gestational vesicle diameter, femur length, placenta thickness, parietal diameter, liver, heart and abdominal diameter and area. Early echo biometric study started at the second week of gestation. Measurements like fetal heart and liver diameter and area are still poorly studied, but can provide useful information for early detection of congenital anomalies that may reduce the viability of pregnancy. The significant results (P &lt; 0.001) obtained for biometrics (P &lt; 0.001) of the parietal (r<sup>2</sup> = 81%) and abdominal diameter (r<sup>2</sup> = 86%), abdominal area (r<sup>2</sup> = 80%), femur length (r<sup>2</sup> = 84%), cardiac length (r<sup>2</sup> = 79%), width (r<sup>2</sup> = 79%), area (r<sup>2</sup> = 82%) and volume (r<sup>2</sup>= 72%) and liver area (r<sup>2</sup> = 71%) in brachycephalic conceptus may help to assess the development of fetuses, complementing the conventional gestational ultrasound of bitches and become a model for the study in other breeds of dogs and alternative animal species.
DOI: 10.6084/m9.figshare.7513466
title: PREVALENCE OF ARBOVIRUS ANTIBODIES AGAINST THE FAMILY Bunyaviridae IN WATER BUFFALOES

description: AbstractThe State of Pará comprises 26% of Brazilian Amazon region where a large diversity of arboviruses has been described. This study sought to assess the prevalence and distribution of haemagglutination-inhibition antibodies against antigens of nine different types of arbovirus of the Bunyaviridae family, where eight were Orthobunyavirus: Guaroa virus, Maguari virus, Tacaiuma virus, Utinga virus, Belem virus, Caraparu virus, Oropouche virus and Catu virus, and one Phlebovirus: Icoaraci virus in sera samples of water buffaloes in Pará State, Brazil. For all Arboviruses investigated there were antibodies, with the exception of Belem virus. Antibodies to Maguari viruswere more prevalent (7.33%). The water buffaloes of the present study showed variable levels of antibodies in monotypic and heterotypic reactions that may indicate there are movements from most bunyavirus studied in domestic buffaloes in the state of Pará, and the Maguari virus presents the largest circulation. Therefore, further studies are needed to investigate the role of water buffalo in the maintenance and dispersal of arboviruses, as well as whether these viruses can cause disease in that species, especially in cases of birth defects and abortions.
DOI: 10.6084/m9.figshare.7509497.v1
title: COMPONENTS OF (CO)VARIANCE FOR AGE AT FIRST AND SECOND CALVING OF NELLORE FEMALES RAISED IN SOUTHERN BRAZIL

description: Abstract The objective of this study was to estimate the heritability, genetic correlation and estimated breeding values for age at first (AFC) and second calving (ASC) for Nellore females raised in Southern Brazil. The (co)variance and estimated breeding values were obtained using Bayesian inference in a bivariate analysis, adopting an animal model. The average ages were 49.30 and 69.85 months, and the heritabilities were 0.25 and 0.26, respectively for AFC and ASC. The genetic correlation between AFC and ASC was 0.88. The correlation between the classifications of sires according to their estimated breeding values was 0.93. The heritability estimates for AFC and ASC suggest the possibility of obtaining genetic gain by selection. The correlation between these traits close to one indicates that they are virtually controlled by the same genes, and the selection for one of them will promote correlated advanced gain for the other.
DOI: 10.6084/m9.figshare.7509497
title: COMPONENTS OF (CO)VARIANCE FOR AGE AT FIRST AND SECOND CALVING OF NELLORE FEMALES RAISED IN SOUTHERN BRAZIL

description: Abstract The objective of this study was to estimate the heritability, genetic correlation and estimated breeding values for age at first (AFC) and second calving (ASC) for Nellore females raised in Southern Brazil. The (co)variance and estimated breeding values were obtained using Bayesian inference in a bivariate analysis, adopting an animal model. The average ages were 49.30 and 69.85 months, and the heritabilities were 0.25 and 0.26, respectively for AFC and ASC. The genetic correlation between AFC and ASC was 0.88. The correlation between the classifications of sires according to their estimated breeding values was 0.93. The heritability estimates for AFC and ASC suggest the possibility of obtaining genetic gain by selection. The correlation between these traits close to one indicates that they are virtually controlled by the same genes, and the selection for one of them will promote correlated advanced gain for the other.
DOI: 10.6084/m9.figshare.7506611.v1
title: DESCRIPTION OF SURVEILLANCE ACTIONS IN OUTBREAKS OF AUJESZKY'S DISEASE

description: AbstractAujeszky’s disease (AD) is known for causing huge productive and economical losses in the swine industry. This study focused on describing the actions of the official animal health protection used on identification of AD outbreaks. Two outbreaks that occurred in Cerqueira César city were selected, a case presenting only seropositive animals (outbreak 1) and another one where there were animals with clinical signs of the disease (outbreak 2). The methods used to identify the outbreaks in surveillance actions and to diagnose the epidemiological situation were described. The measures implemented to eliminate the outbreaks in infected pig production systems were appointed in animal health protection laws and were able to eliminated all outbreaks identified. In conclusion, the official animal health protection measures and the appliance of the sanitary legislation were effective in eradicating AD, reassuring that notifications of suspicous cases of AD facilitate animal health surveillance activities.
DOI: 10.6084/m9.figshare.7506611
title: DESCRIPTION OF SURVEILLANCE ACTIONS IN OUTBREAKS OF AUJESZKY'S DISEASE

description: AbstractAujeszky’s disease (AD) is known for causing huge productive and economical losses in the swine industry. This study focused on describing the actions of the official animal health protection used on identification of AD outbreaks. Two outbreaks that occurred in Cerqueira César city were selected, a case presenting only seropositive animals (outbreak 1) and another one where there were animals with clinical signs of the disease (outbreak 2). The methods used to identify the outbreaks in surveillance actions and to diagnose the epidemiological situation were described. The measures implemented to eliminate the outbreaks in infected pig production systems were appointed in animal health protection laws and were able to eliminated all outbreaks identified. In conclusion, the official animal health protection measures and the appliance of the sanitary legislation were effective in eradicating AD, reassuring that notifications of suspicous cases of AD facilitate animal health surveillance activities.