cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11966250.v1
title: Boundaries in metagenomic screenings using lacZα-based vectors

description: Abstract Metagenomics approaches have been of high relevance for providing enzymes used in diverse industrial applications. In the current study, we have focused on the prospection of protease and glycosyl hydrolase activities from a soil sample by using the lacZα -based plasmid pSEVA232. For this, we used a functional screen based on skimmed milk agar and a pH indicator dye for detection of both enzymes, as previously reported in literature. Although we effectively identified positive clones in the screenings, subsequent experiments revealed that this phenotype was not because of the hydrolytic activity encoded in the metagenomic fragments, but rather due to the insertion of small metagenomic DNA fragments in frame within the coding region of the lacZ gene present in the original vector. Analyses of the thermodynamic stability of mRNA secondary structures indicated that recovering of positive clones was probably due to higher expression levels of the chimeric lacZα-genes in respect to the original from empty vector. We concluded that this method has a higher tendency for recovery false positive clones, when used in combination with a lacZα-based vector. As these vectors are massively used in functional metagenomic screenings, we highlight the importance of reporting boundaries in established metagenomic screenings methodologies.
DOI: 10.6084/m9.figshare.11966247.v1
title: Association between Genetic Polymorphisms in RANK, RANKL and OPG and Peri-Implant Diseases in Patients from the Amazon Region

description: Abstract The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status: Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p&lt;0.05) and peri-implantitis (p&lt;0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p&lt;0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p&gt;0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region.
DOI: 10.6084/m9.figshare.11966226
title: The Copernican Revolution: historical and epistemological aspects

description: Abstract In this paper we make a didactic presentation of the so-called Copernican Revolution, highlighting some historical and epistemological aspects associated with it. In particular, we seek to show how the history of astronomical science, in which Copernicus' heliocentric model is inserted, has always been subject to a dispute between two scientific conceptions, which we might call realism and instrumentalism, especially as regards Ptolemy's use of devices such as the equant and eccentric in describing planetary motions. We give a brief exposition of Aristotle's cosmological thinking and the geocentric astronomical models of Ptolemy and his Arab successors, and then a more detailed, though qualitative, exposition of Copernicus's new proposal as well as its epistemological foundations. Finally, we also seek to show how this proposal, in principle restricted to a specific field of knowledge, completely shook the widely prevailing Aristotelian scientific and philosophical conception, constituting yet another element of confrontation between a new emerging worldview and another, already in its last moments, a conflict that was reflected strongly in the religious domain.
DOI: 10.6084/m9.figshare.11966229
title: Social innovation ecosystems, sustainability, and democratic experimentation: a study in Florianopolis, Brazil

description: Abstract Cities have been conceived as one of the main loci for promoting changes in development patterns, and building solutions that can address the complexity of contemporary public problems. In this context, the study of social innovation ecosystems (SIE) and their effects on cities is a relevant theme, considering the socio-environmental crisis diversity and extent of the urban problems experienced. The research seeks to understand to what extent the SIE responds to problems experienced in the public arenas of the city. It proposes a pragmatist-inspired theoretical-methodological approach to cartography and analysis of SIE that is being applied in the city of Florianópolis. Through the co-construction of a collaborative digital platform, the research observes actors, their interactions and practices, to understand “whether” and “how” this network favors “public inquiry” or democratic experimentation and/or promotes more sustainable development dynamics in the city.
DOI: 10.6084/m9.figshare.11966226.v1
title: The Copernican Revolution: historical and epistemological aspects

description: Abstract In this paper we make a didactic presentation of the so-called Copernican Revolution, highlighting some historical and epistemological aspects associated with it. In particular, we seek to show how the history of astronomical science, in which Copernicus' heliocentric model is inserted, has always been subject to a dispute between two scientific conceptions, which we might call realism and instrumentalism, especially as regards Ptolemy's use of devices such as the equant and eccentric in describing planetary motions. We give a brief exposition of Aristotle's cosmological thinking and the geocentric astronomical models of Ptolemy and his Arab successors, and then a more detailed, though qualitative, exposition of Copernicus's new proposal as well as its epistemological foundations. Finally, we also seek to show how this proposal, in principle restricted to a specific field of knowledge, completely shook the widely prevailing Aristotelian scientific and philosophical conception, constituting yet another element of confrontation between a new emerging worldview and another, already in its last moments, a conflict that was reflected strongly in the religious domain.
DOI: 10.6084/m9.figshare.11966229.v1
title: Social innovation ecosystems, sustainability, and democratic experimentation: a study in Florianopolis, Brazil

description: Abstract Cities have been conceived as one of the main loci for promoting changes in development patterns, and building solutions that can address the complexity of contemporary public problems. In this context, the study of social innovation ecosystems (SIE) and their effects on cities is a relevant theme, considering the socio-environmental crisis diversity and extent of the urban problems experienced. The research seeks to understand to what extent the SIE responds to problems experienced in the public arenas of the city. It proposes a pragmatist-inspired theoretical-methodological approach to cartography and analysis of SIE that is being applied in the city of Florianópolis. Through the co-construction of a collaborative digital platform, the research observes actors, their interactions and practices, to understand “whether” and “how” this network favors “public inquiry” or democratic experimentation and/or promotes more sustainable development dynamics in the city.
DOI: 10.6084/m9.figshare.11966220
title: Discussing Ptolemy's model and its equivalence with the Copernican model

description: Abstract We reviewed Ptolemy's geocentric model and showed step by step how to determine the longitude of a planet using a modern mathematical approach to facilitate its understanding. We also discussed Ptolemy's considerations for fixing the planets' latitude value. A geometric equivalence between the Ptolemy and Copernicus models are presented, highlighting the restrictions with which this equivalence was built. Furthermore, the aspects associated with the transition between Ptolemy's geocentric model and Copernicus's heliocentric model are discussed. We considered aspects about the use of epicycles over epicycles, as well as the question of the precision of the Ptolemy model in relation to the Copernicus model. In general, in this work we present a quantitative and qualitative view of Ptolemy's model so that the reader has a simple mathematical formulation available to determine the position of the planets. Additionally, to understand how Copernicus' proposal, even though it is geometrically equivalent to Ptolemy's model, turned out to be a physically more complete model and of a different aesthetic (movement of the Earth around the Sun) capable of unifying concepts.
DOI: 10.6084/m9.figshare.11966220.v1
title: Discussing Ptolemy's model and its equivalence with the Copernican model

description: Abstract We reviewed Ptolemy's geocentric model and showed step by step how to determine the longitude of a planet using a modern mathematical approach to facilitate its understanding. We also discussed Ptolemy's considerations for fixing the planets' latitude value. A geometric equivalence between the Ptolemy and Copernicus models are presented, highlighting the restrictions with which this equivalence was built. Furthermore, the aspects associated with the transition between Ptolemy's geocentric model and Copernicus's heliocentric model are discussed. We considered aspects about the use of epicycles over epicycles, as well as the question of the precision of the Ptolemy model in relation to the Copernicus model. In general, in this work we present a quantitative and qualitative view of Ptolemy's model so that the reader has a simple mathematical formulation available to determine the position of the planets. Additionally, to understand how Copernicus' proposal, even though it is geometrically equivalent to Ptolemy's model, turned out to be a physically more complete model and of a different aesthetic (movement of the Earth around the Sun) capable of unifying concepts.
DOI: 10.6084/m9.figshare.11966202
title: Comparing the postoperative refractive predictability of Pentacam HR and IOLMaster 500 after a multifocal intraocular lens implantation

description: ABSTRACT Purpose: To compare the postoperative refractive predictability of IOLMaster 500 and Pentacam HR on the basis of keratometry and anterior chamber depth values in eyes with an indication for multifocal intraocular lens (IOL) implantation. Methods: This was a retrospective study conducted on 118 eyes treated with phacoemulsification and multifocal intraocular lens implantation. Only the eyes that achieved emmetropia in the dynamic refraction performed on postoperative day 30 were included. Haigis’ formula was used in each case to calculate the intraocular lens power, and the intraocular lens with the target refraction closest to emmetropia was implanted. Four lens calculation scenarios were tested by combining keratometry and anterior chamber depth measurements obtained using the two devices. Results: IOLMaster 500 and Pentacam HR differed with regard to mean keratometry (D 0.07 ± 0.03 D; p=0.0065) and anterior chamber depth (D 0.08 ± 0.01 mm; p&lt;0.001). In the analysis of covariance, the following differences were obtained using the Haigis’ formula when confronted with the biometric values obtained by inserting keratometry and anterior chamber depth values, respectively: Penta/IOL x IOL/Penta (0.13 ± 0.03; p&lt;0.0001); Penta/Penta × IOL/Penta (0.13 ± 0.03; p&lt;0.0001); Penta/IOL × IOL/IOL (0.11 ± 0.03; p=0.001); Penta/Penta × IOL/IOL (0.11 ± 0.03; p=0.002); IOL/IOL × IOL/Penta (0.02 ± 0.03; p=0.865); and Penta/IOL × Penta/Penta (0.002 ± 0.03; p=0.99). The difference was smaller when measuring the anterior chamber depth using the IOLMaster 500, regardless of which device was used to measure keratometry. Conclusions: Pentacam HR significantly differed from IOLMaster 500 when calculating keratometry. As regards the anterior chamber depth, the two devices were equally accurate.
DOI: 10.6084/m9.figshare.11966202.v1
title: Comparing the postoperative refractive predictability of Pentacam HR and IOLMaster 500 after a multifocal intraocular lens implantation

description: ABSTRACT Purpose: To compare the postoperative refractive predictability of IOLMaster 500 and Pentacam HR on the basis of keratometry and anterior chamber depth values in eyes with an indication for multifocal intraocular lens (IOL) implantation. Methods: This was a retrospective study conducted on 118 eyes treated with phacoemulsification and multifocal intraocular lens implantation. Only the eyes that achieved emmetropia in the dynamic refraction performed on postoperative day 30 were included. Haigis’ formula was used in each case to calculate the intraocular lens power, and the intraocular lens with the target refraction closest to emmetropia was implanted. Four lens calculation scenarios were tested by combining keratometry and anterior chamber depth measurements obtained using the two devices. Results: IOLMaster 500 and Pentacam HR differed with regard to mean keratometry (D 0.07 ± 0.03 D; p=0.0065) and anterior chamber depth (D 0.08 ± 0.01 mm; p&lt;0.001). In the analysis of covariance, the following differences were obtained using the Haigis’ formula when confronted with the biometric values obtained by inserting keratometry and anterior chamber depth values, respectively: Penta/IOL x IOL/Penta (0.13 ± 0.03; p&lt;0.0001); Penta/Penta × IOL/Penta (0.13 ± 0.03; p&lt;0.0001); Penta/IOL × IOL/IOL (0.11 ± 0.03; p=0.001); Penta/Penta × IOL/IOL (0.11 ± 0.03; p=0.002); IOL/IOL × IOL/Penta (0.02 ± 0.03; p=0.865); and Penta/IOL × Penta/Penta (0.002 ± 0.03; p=0.99). The difference was smaller when measuring the anterior chamber depth using the IOLMaster 500, regardless of which device was used to measure keratometry. Conclusions: Pentacam HR significantly differed from IOLMaster 500 when calculating keratometry. As regards the anterior chamber depth, the two devices were equally accurate.
DOI: 10.6084/m9.figshare.11966193
title: Culture of people originating from the Amazon rainforest during pregnancy and the puerperium: a scope review from the point of view of food and nutrition security

description: ABSTRACT Malnutrition in the indigenous population is a current public health problem and a major cause of morbidity and mortality of children in these groups in Brazil. In order to support actions to promote food and nutrition education, including forest peoples from the state of Amazonas, Brazil, whose territory borders Colombia, in addition to the field visit in 2018, it was necessary, at the same time, to map the literature as this one. Population lives and how care is provided. The scope review sought topics related to the lifestyle of women and children in the Amazon, diet during pregnancy and the postpartum period, breastfeeding practices, the introduction of solid foods to the baby, and the care of health services. As a result, 21 multidisciplinary studies were retrieved. Traditional food has been found to have higher nutritional value than industrialized food, and local cuisine is a source of income for indigenous women in the urban environment and a link between ethnicities. It is concluded that the researches should incorporate the health promotion paradigm and cover themes such as indigenous acculturation in the Amazonian urban centers, the arrival of the internet in the villages and the role of distance care, which needs to be investigated to better address the problem.
DOI: 10.6084/m9.figshare.11966193.v1
title: Culture of people originating from the Amazon rainforest during pregnancy and the puerperium: a scope review from the point of view of food and nutrition security

description: ABSTRACT Malnutrition in the indigenous population is a current public health problem and a major cause of morbidity and mortality of children in these groups in Brazil. In order to support actions to promote food and nutrition education, including forest peoples from the state of Amazonas, Brazil, whose territory borders Colombia, in addition to the field visit in 2018, it was necessary, at the same time, to map the literature as this one. Population lives and how care is provided. The scope review sought topics related to the lifestyle of women and children in the Amazon, diet during pregnancy and the postpartum period, breastfeeding practices, the introduction of solid foods to the baby, and the care of health services. As a result, 21 multidisciplinary studies were retrieved. Traditional food has been found to have higher nutritional value than industrialized food, and local cuisine is a source of income for indigenous women in the urban environment and a link between ethnicities. It is concluded that the researches should incorporate the health promotion paradigm and cover themes such as indigenous acculturation in the Amazonian urban centers, the arrival of the internet in the villages and the role of distance care, which needs to be investigated to better address the problem.
DOI: 10.6084/m9.figshare.11966190
title: Regression of macular edema with topical brinzolamide and nepafenac alone and identification of a novel gyrate atrophy mutation

description: ABSTRACT Gyrate atrophy is a rare metabolic autosomal recessive disorder caused by ornithine aminotransferase enzyme deficiency that leads to characteristic progressive, degenerative chorioretinal findings. Patients complain mostly of low vision, night blindness, and peripheral vision loss. Posterior subcapsular cataract, myopia, choroid neovascularization, and intraretinal cysts may be accompanying factors related to vision loss. We encountered a patient with vision loss secondary to posterior subcapsular cataract and intraretinal cysts. After treatment with topical brinzolamide and nepafenac (and without any diet mo dification and/or supplementation), we observed 143- and 117-mm macular thickness resolutions with 2 and 1 Snellen lines of visual gain in his right and left eyes, respectively. Also, we detected a novel homozygous mutation in the ornithine aminotransferase gene: c.1253T&gt;C (p.Leu418Pro). Carbonic anhydrase inhibitors and/or non-steroid anti-inflammatory drugs can control macular edema in patients with gyrate atrophy-associated intraretinal cysts. The genetic variants may also be a determinant in the responsiveness to the therapy type.
DOI: 10.6084/m9.figshare.11966190.v1
title: Regression of macular edema with topical brinzolamide and nepafenac alone and identification of a novel gyrate atrophy mutation

description: ABSTRACT Gyrate atrophy is a rare metabolic autosomal recessive disorder caused by ornithine aminotransferase enzyme deficiency that leads to characteristic progressive, degenerative chorioretinal findings. Patients complain mostly of low vision, night blindness, and peripheral vision loss. Posterior subcapsular cataract, myopia, choroid neovascularization, and intraretinal cysts may be accompanying factors related to vision loss. We encountered a patient with vision loss secondary to posterior subcapsular cataract and intraretinal cysts. After treatment with topical brinzolamide and nepafenac (and without any diet mo dification and/or supplementation), we observed 143- and 117-mm macular thickness resolutions with 2 and 1 Snellen lines of visual gain in his right and left eyes, respectively. Also, we detected a novel homozygous mutation in the ornithine aminotransferase gene: c.1253T&gt;C (p.Leu418Pro). Carbonic anhydrase inhibitors and/or non-steroid anti-inflammatory drugs can control macular edema in patients with gyrate atrophy-associated intraretinal cysts. The genetic variants may also be a determinant in the responsiveness to the therapy type.
DOI: 10.6084/m9.figshare.11966175
title: Evidence of autophagic vesicles in a patient with Lisch corneal dystrophy

description: ABSTRACT Lisch corneal dystrophy is a rare corneal disease characterized by the distinctive feature of highly vacuolated cells. Although this feature is important, the nature of these vacuoles within corneal cells remains unknown. Here, we sought to analyze corneal cells from a patient diagnosed with Lisch dystrophy to characterize the vacuoles within these cells. Analyses using histopathology examination, confocal microscopy, and transmission electron microscopy were all consistent with previous descriptions of Lisch cells. Importantly, the vacuoles within these cells appeared to be autophagosomes and autolysosomes, and could be stained with an anti-microtubule-associated protein 1A/1B-light chain 3 (LC3) antibody. Taken together, these findings indicate that the vacuoles we observed within superficial corneal cells of a patient with Lisch corneal dystrophy constituted autophagosomes and autolysosomes; this finding has not been previously reported and suggests a need for further analyses to define the role of autophagy in this ocular disease.
DOI: 10.6084/m9.figshare.11966175.v1
title: Evidence of autophagic vesicles in a patient with Lisch corneal dystrophy

description: ABSTRACT Lisch corneal dystrophy is a rare corneal disease characterized by the distinctive feature of highly vacuolated cells. Although this feature is important, the nature of these vacuoles within corneal cells remains unknown. Here, we sought to analyze corneal cells from a patient diagnosed with Lisch dystrophy to characterize the vacuoles within these cells. Analyses using histopathology examination, confocal microscopy, and transmission electron microscopy were all consistent with previous descriptions of Lisch cells. Importantly, the vacuoles within these cells appeared to be autophagosomes and autolysosomes, and could be stained with an anti-microtubule-associated protein 1A/1B-light chain 3 (LC3) antibody. Taken together, these findings indicate that the vacuoles we observed within superficial corneal cells of a patient with Lisch corneal dystrophy constituted autophagosomes and autolysosomes; this finding has not been previously reported and suggests a need for further analyses to define the role of autophagy in this ocular disease.
DOI: 10.6084/m9.figshare.11966160
title: Cytotoxicity of Chelating Agents Used In Endodontics and Their Influence on MMPs of Cell Membranes

description: Abstract This study evaluated the cytotoxic effect and the ability to inhibit matrix metalloproteinases (MMP-2 and MMP-9) of 0.2% chitosan (CH) and 1% acetic acid (AA) compared with 17% ethylenediaminetetraacetic acid (EDTA). Cell viability assay was performed according to ISO 10993-5 with mouse fibroblasts (L929). The culture was exposed to 0.2% CH, 1% AA, and 17% EDTA. The chelating agents were evaluated immediately after contact with the cells and after 6 h, 12 h, and 24 h of incubation. Cell viability was analyzed using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Inhibition of the gelatinolytic activity of MMP-2 and MMP-9 was evaluated by gelatin zymography. Different concentrations of CH were evaluated: 50 mM, 5 mM, 0.5 mM, and 0.05 mM. EDTA (0.5 mM) was used as a positive control. The results demonstrated that CH and AA had an initial cytotoxic effect, which decreased after 6 h, 12 h, and 24 h, being statistically similar to EDTA (P &gt; 0.05). Additionally, CH at concentrations of 50 mM, 5 mM, and 0.5 mM had an inhibitory effect on MMP-2 and MMP-9, similar to that of the control with EDTA. The chelating agents had no cytotoxic effects after 24 h. MMP-2 and MMP-9 were inhibited by the experimental solutions.