cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.3535/g99-w1d-y1c
title: Atys naucum (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gve-61e-vxt
title: Albinaria senilis epirotica (Mousson, 1859)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/czm-zf0-jaj
title: Alopia lischkeana (Charpentier, 1852)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/50e-9ms-89y
title: Retusidae Thiele, 1925

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/z6m-0kd-kwf
title: Ocenebra erinaceus (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/2vt-64c-t0g
title: Jaminia quadridens (O. F. Müller, 1774)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/tb4-29z-svp
title: Agathylla sulcosa (J. A. Wagner, 1829)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/r7k-f5d-8rp
title: Granaria variabilis (Draparnaud, 1801)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/bgx-xga-j7t
title: Macrogastra rolphii (W. Turton, 1826)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jp1-yfq-b7y
title: Macrogastra ventricosa (Draparnaud, 1801)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/1br-dmr-fjl
title: Gastrocopta barbadensis barbadensis (L. Pfeiffer, 1853)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/cmc-qr1-zgs
title: Laminella citrina (L. Pfeiffer, 1848)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/fn8-yyw-83e
title: Walklea rossmaessleri (Rossmässler, 1838)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/q2m-cj3-46q
title: Pagodulina austeniana austeniana (G. Nevill, 1880)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/tq9-a2d-j4j
title: Pythia scarabaeus (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/d6m-ryd-seh
title: Psilaxis radiatus (Röding, 1798)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/vy6-jng-gtz
title: Turbonilla Risso, 1826

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/6ny-h62-sm8
title: Macrogastra plicatula (Draparnaud, 1801)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/det-fer-b8r
title: Vallonia costata (O. F. Müller, 1774)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/e39-dw9-l7r
title: Pecten jacobaeus (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/h9j-7yp-jxn
title: Bullidae Gray, 1827

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/2q4-8v8-xdz
title: Akera bullata O. F. Müller, 1776

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/9se-cn0-xbz
title: Albinaria senilis flavescens (O. Boettger, 1878)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/08b-p3e-v8s
title: Berthella plumula (Montagu, 1803)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/txj-zf4-ssj
title: Zebrina detrita (O. F. Müller, 1774)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/xzf-ay8-jf2
title: Lepidochitona cinerea (Linnaeus, 1767)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/hkw-8dw-4np
title: Alopia nixa (M. Kimakowicz, 1894)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/pf2-wa5-ela
title: Carychium bermudense Gulick, 1904

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/e0v-bzq-sre
title: Chondrina avenacea avenacea (Bruguière, 1792)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/nf7-5c4-eh8
title: Alopia plumbea (Rossmässler, 1839)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/0x1-5xz-87m
title: Granopupa granum (Draparnaud, 1801)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/jmw-qy2-3bx
title: Orculella connollyi (Brandt, 1956)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/t9x-vq8-njm
title: Lyromangelia taeniata (Deshayes, 1835)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/gfn-m9l-l5z
title: Leptachatina perkinsi Sykes, 1896

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/ll8-jaw-xrk
title: Gastrocopta oblonga (L. Pfeiffer, 1854)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.3535/m2n-2qw-41e
title: Radix auricularia (Linnaeus, 1758)

description: Digital Specimen for the physical specimen hosted at Naturalis Biodiversity Center.
DOI: 10.15468/dl.8rctd6
title: Occurrence Download

description: A dataset containing 8872 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Acalypha australis"
 ]
}

 The dataset includes 8872 records from 247 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008399-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/14463654
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.25549/inscriptifact-ouc112053975
title: Baal Myth Sixth Tablet

description: [Text Description:] Shapsh helps Anat recover Baal's corpse, Anat destroys Mot, Baal is restored and fights Mot ❧ [Text or Publication Number Note:] KTU 1.6; CTA 6; CAT 1.6; KTU2 1.6; CTU 1.6; RS 2.[009]; RS 5.155 ❧ [Excavation Description:] Excavations at Ras Shamra 1930. Found in Library of the High Priest, p. t. 210-264, Acropolis, excavated by Claude F.-A. Schaeffer, Mission Archeologique Francaise. ❧ [Medium:] Clay ❧ [Script Note:] Alphabetic Cuneiform ❧ [Physical Object Description:] Clay tablet, 26.5 cm. X 19.5 cm., 2 fragments ❧ [Physical Object Note:] Grey clay, six columns, incomplete
DOI: 10.15468/dl.e9jf8c
title: Occurrence Download

description: A dataset containing 40 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Selago elongata Hilliard"
 ]
}

 The dataset includes 40 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0122801-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gpz8pd
title: Occurrence Download

description: A dataset containing 14185 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-76.71432 5.99361,-76.80452 5.53149,-76.45833 5.10061,-75.7252 5.02137,-75.30482 5.26766,-74.971 5.59998,-74.84736 6.16573,-75.00809 6.64492,-75.67574 6.75543,-76.59068 6.37466,-76.71432 5.99361))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Mammalia"
 ]
}

 The dataset includes 14185 records from 79 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0032312-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3wjcbu
title: Occurrence Download

description: A dataset containing 17 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Asia",
 "Country is India",
 "TaxonKey is Pomadasys kaakan (Cuvier, 1830)"
 ]
}

 The dataset includes 17 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0398092-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.g3fkfn
title: Occurrence Download

description: A dataset containing 258 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Platysteira chalybea (Reichenow, 1897)"
 ]
}

 The dataset includes 258 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0382635-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.smf2yv
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Takamatsuella circinata (Cooke & Peck) U.Braun & A.N.Shi",
 {
 "not" : {
 "DatasetKey" : [
 "is Global database of alien macrofungi"
 ]
 }
 },
 {
 "or" : [
 "Country is Switzerland",
 "Country is Switzerland"
 ]
 }
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0401868-210914110416597/datasets/export for details.
DOI: 10.25549/inscriptifact-ouc112053930
title: Baal Myth Sixth Tablet

description: [Text Description:] Shapsh helps Anat recover Baal's corpse, Anat destroys Mot, Baal is restored and fights Mot ❧ [Text or Publication Number Note:] KTU 1.6; CTA 6; CAT 1.6; KTU2 1.6; CTU 1.6; RS 2.[009]; RS 5.155 ❧ [Excavation Description:] Excavations at Ras Shamra 1930. Found in Library of the High Priest, p. t. 210-264, Acropolis, excavated by Claude F.-A. Schaeffer, Mission Archeologique Francaise. ❧ [Medium:] Clay ❧ [Script Note:] Alphabetic Cuneiform ❧ [Physical Object Description:] Clay tablet, 26.5 cm. X 19.5 cm., 2 fragments ❧ [Physical Object Note:] Grey clay, six columns, incomplete
DOI: 10.15468/dl.2wknk8
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Ceratocystis variospora (R.W.Davidson) C.Moreau",
 {
 "not" : {
 "DatasetKey" : [
 "is Global database of alien macrofungi"
 ]
 }
 },
 {
 "or" : [
 "Country is Switzerland",
 "Country is Switzerland"
 ]
 }
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0401870-210914110416597/datasets/export for details.
DOI: 10.15468/dl.jpgcja
title: Occurrence Download

description: A dataset containing 29 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Selago immersa Rolfe"
 ]
}

 The dataset includes 29 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0122819-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.r7b659
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Phomopsis glandicola (Lév.) Grove",
 {
 "not" : {
 "DatasetKey" : [
 "is Global database of alien macrofungi"
 ]
 }
 },
 {
 "or" : [
 "Country is Switzerland",
 "Country is Switzerland"
 ]
 }
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0401780-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112053760
title: Baal Myth Fifth Tablet

description: [Text Description:] Baal enters underworld, his body is discovered, El and Anat mourn him ❧ [Text or Publication Number Note:] KTU 1.5; CTA 5; CAT 1.5; KTU2 1.5; CTU 1.5; RS 2.[022]; RS 3.[565] ❧ [Excavation Description:] Excavations at Ras Shamra 1930. Found in Library of the High Priest, p. t. 210-264, Acropolis, excavated by Claude F.-A. Schaeffer, Mission Archeologique Francaise. ❧ [Medium:] Clay ❧ [Script Note:] Alphabetic Cuneiform ❧ [Physical Object Description:] Clay tablet, 14.5 cm. X 15.6 cm., 6 fragments ❧ [Physical Object Note:] Grey clay, six columns, incomplete
DOI: 10.15468/dl.yxqp9v
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Batis reichenowi Grote, 1911"
 ]
}

 The dataset includes 6 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0382610-210914110416597/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/inscriptifact-ouc112020496
title: Cuneiform Tablet 1913.14.1501, Spurlock 1913.14.1501

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.1501; CDLI P421353 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish White (10YR 9/1.5) Grayish Reddish Orange (10R 5/6) Medium Gray (N 6/ 30.0% R) ❧ [Physical Object Note:] 6.8 x 4.8 x 2 cm (61g)
DOI: 10.25549/inscriptifact-ouc112045359
title: Cuneiform Tablet 1913.14.0169, Spurlock 1913.14.0169

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0169; CDLI P420550 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish White (10YR 9/2) Brownish Pink (5YR 8/3) ❧ [Physical Object Note:] 7.5 x 4.5 x 2.3 cm (82g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.25549/inscriptifact-ouc112045282
title: Cuneiform Tablet 1913.14.0169, Spurlock 1913.14.0169

description: [Text Description:] Administrative tablet ❧ [Text or Publication Number Note:] Spurlock 1913.14.0169; CDLI P420550 ❧ [Medium:] Clay ❧ [Script Note:] Non-Alphabetic Cuneiform ❧ [Physical Object Description:] Yellowish White (10YR 9/2) Brownish Pink (5YR 8/3) ❧ [Physical Object Note:] 7.5 x 4.5 x 2.3 cm (82g) ❧ [Physical Object Note:] Seal impressions
DOI: 10.15468/dl.rnjyvh
title: Occurrence Download

description: A dataset containing 29758955 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is one of (Gyrostemonaceae, Haemodoraceae, Halophytaceae, Halophilaceae, Haloragaceae, Haloragidaceae, Halogaraceae, Hamamelidaceae, Hanguanaceae, Heliconiaceae, Helwingiaceae, Hedwigiaceae, Hemidictyaceae, Hernandiaceae, Hermanniaceae, Harmandiaceae, Himantandraceae, Huaceae, Hsuaceae, Humiriaceae, Hydatellaceae, Hydrangeaceae, Hydrageaceae, Hydrocharitaceae, Hydroleaceae, Hydrageaceae, Hydrillaceae, Hydroceraceae, Hydrostachyaceae, Hymenophyllaceae, Hymenophytaceae, Hypericaceae, Hypodematiaceae, Hypoxidaceae, Icacinaceae, Iridaceae, Irvingiaceae, Isoetaceae, Iteaceae, Ixioliriaceae, Ixonanthaceae, Joinvilleaceae, Juglandaceae, Juncaceae, Juncaginaceae, Kewaceae, Kirkiaceae, Koeberliniaceae, Krameriaceae, Lacistemataceae, Lamiaceae, Lasiaceae, Lanariaceae, Lardizabalaceae, Lauraceae, Lecythidaceae, Lentibulariaceae, Lepidobotryaceae, Liliaceae, Limeaceae, Limnanthaceae, Linaceae, Linderniaceae)"
 ]
}

 The dataset includes 29758955 records from 7523 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0145607-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.17491645
title: Spatially Explicit Machine Learning for Mapping Soil Heavy Metals in Abandoned Rare Earth Mines Using Multisource Remote Sensing Data

description: This dataset contains two geospatial layers used in the study “Spatially Explicit Machine Learning for Mapping Soil Heavy Metals in Abandoned Rare Earth Mines Using Multisource Remote Sensing Data.”The database is provided in ESRI Shapefile format and includes:

1. Study area boundary (gannan_ree_polygon_of_study_area.zip)A polygon dataset defining the spatial extent of the study area.It was used for raster clipping, masking, and generating prediction maps.

2. Sampling points (gannan_ree_sample_points.zip)A point dataset representing soil sampling locations within the study region.Attribute fields include sample ID and associated soil heavy-metal measurements used for model training and validation.

Both layers are spatially referenced and ready for direct use in GIS and geospatial machine learning workflows.
DOI: 10.5281/zenodo.17491646
title: Spatially Explicit Machine Learning for Mapping Soil Heavy Metals in Abandoned Rare Earth Mines Using Multisource Remote Sensing Data

description: This dataset contains two geospatial layers used in the study “Spatially Explicit Machine Learning for Mapping Soil Heavy Metals in Abandoned Rare Earth Mines Using Multisource Remote Sensing Data.”The database is provided in ESRI Shapefile format and includes:

1. Study area boundary (gannan_ree_polygon_of_study_area.zip)A polygon dataset defining the spatial extent of the study area.It was used for raster clipping, masking, and generating prediction maps.

2. Sampling points (gannan_ree_sample_points.zip)A point dataset representing soil sampling locations within the study region.Attribute fields include sample ID and associated soil heavy-metal measurements used for model training and validation.

Both layers are spatially referenced and ready for direct use in GIS and geospatial machine learning workflows.
DOI: 10.6075/j03j3bnp
title: CIL:13892, Saccharomyces cerevisiae. In Cell Image Library

description: Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: HA peptide tag; 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody; Cy3; Fluorescein (FITC)
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Cells (MATa bfa1::3HA-BFA1) grown in rich media with glucose were fixed for 15 min in 3.7% formaldehyde and 0.1 M potassium phosphate buffer, pH 6.4. Cells were then washed twice with 0.1 M potassium phosphate buffer, pH 6.4, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4/0.033 M citric acid, pH 5.9. Fixed cells were digested with 0.1 mg/ml zymolyase-100T (US Biological) and 1/10 volume of glusulase (PerkinElmer) at 30C for 15 min, washed once, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4/0.033 M citric acid, pH 5.9. Primary antibodies were anti-HA monoclonal antibody (HA.11; 1:500) and anti-tubulin (Abcam; 1:500). Secondary antibodies were: anti-mouse Cy3 (for HA) and anti-rat FITC (for tubulin). Cells were resuspended in DAPI (1 mg/ml). Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.
description: 3HA-Bfa1 (red) is generally localized to the daughter spindle pole body (SPB) in wild-type cells in anaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control image for CIL# 13893 in which constitutive targeting of Tem1 to both SPBs leads to symmetric SPB localization of Bfa1. Image is Fig 4D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 4 include CIL# 13890, 13891, 13892, 13893.
DOI: 10.6075/j0j96536
title: CIL:13887, Saccharomyces cerevisiae. In Cell Image Library

description: Cells (MATa tem1::GAL-UPL-TEM1-TRP1 pRS316::eGFP-BFA1–TEM1) were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software.
description: Preparation: formaldehyde fixed tissue; ethanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: widefield illumination; fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; compartmentalization of stain or label; boundaries between regions with different refractive index
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (CIL# 13884) and anaphase cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-BFA1–TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose. Image shows localization of Bfa1-Tem1 (eGFP, green) in anaphase after cells were transferred to medium with 2% glucose. Tem1 normally localizes preferentially to the SPB that enters the daughter cell during anaphase (CIL# 13882, 13885). Localization of Bfa1 is similar to that of Tem1, but Bfa1 is more stable on the SPB in the daughter cell and more asymmetric than Tem1 in late anaphase. The Bfa1-Tem1 fusion protein localization resembles Bfa1. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1B, bottom panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.