cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25345/c5k753
title: MassIVE MSV000086068 - Conditioned media of naive human pluripotent stem cells cultured in microfluidics and RSet medium
title: These are 3 TMT10 samples. The indicated "standard" sub-samples all refer to the same biological sample (obtained as a mixture of all other samples), split and tagged as indicated, to play as a reference for normalization. TMT10 sample1 contains the following sub-samples: -TAG 126: Standard. -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 128C: Not relevant. -TAG 129N: Not relevant. -TAG 129C: Not relevant. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. TMT10 sample2 contains the following sub-samples: -TAG 126: Standard -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 128C: Not relevant. -TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. TMT10 sample3 contains the following sub-samples: -TAG 126: Standard. -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 128C: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. Detailed culture and processing methods are described in Cesare et al, under submission.
DOI: 10.6084/m9.figshare.3619749
title: Value-based design for the elderly: An application in the field of mobility aids

description: In the aging society, the need for the elderly to remain mobile and independent is higher than ever. However, many aids supporting mobility often fail to target real needs and lack acceptance. The aim of this study is to demonstrate how value-based design can contribute to the design of mobility aids that address real needs and thus, lead to high acceptance. We elicited values, facilitators, and barriers of mobility of older adults via ten in-depth interviews. Next, we held co-creation sessions, resulting in several designs of innovative mobility aids, which were evaluated for acceptance via nine in-depth interviews. The interviews resulted in a myriad of key values, such as “independence from family” and “doing their own groceries.” Design sessions resulted in three designs for a wheeled walker. Their acceptance was rather low. Current mobility device users were more eager to accept the designs than non-users. The value-based approach offers designers a close look into the lives of the elderly, thereby opening up a wide range of innovation possibilities that better fit their actual needs. Product service systems seem to be a promising focus for targeting human needs in mobility device design.
DOI: 10.6084/m9.figshare.3619749.v2
title: Value-based design for the elderly: An application in the field of mobility aids

description: In the aging society, the need for the elderly to remain mobile and independent is higher than ever. However, many aids supporting mobility often fail to target real needs and lack acceptance. The aim of this study is to demonstrate how value-based design can contribute to the design of mobility aids that address real needs and thus, lead to high acceptance. We elicited values, facilitators, and barriers of mobility of older adults via ten in-depth interviews. Next, we held co-creation sessions, resulting in several designs of innovative mobility aids, which were evaluated for acceptance via nine in-depth interviews. The interviews resulted in a myriad of key values, such as “independence from family” and “doing their own groceries.” Design sessions resulted in three designs for a wheeled walker. Their acceptance was rather low. Current mobility device users were more eager to accept the designs than non-users. The value-based approach offers designers a close look into the lives of the elderly, thereby opening up a wide range of innovation possibilities that better fit their actual needs. Product service systems seem to be a promising focus for targeting human needs in mobility device design.
DOI: 10.6084/m9.figshare.3619749.v1
title: Value-Based Design for the Elderly: An Application in the Field of Mobility Aids

description: <i>Background</i>. In our aging society, the need for the elderly to remain mobile and independent is higher than ever. However, many aids supporting mobility often fail to target real needs and lack acceptance. <i>Aim</i>. The aim of this study is to demonstrate how value-based design can contribute to the design of mobility aids that address real needs and thus, lead to high acceptance. <i>Material and methods</i>: We elicited values, facilitators and barriers of mobility of older adults via ten in-depth interviews. Next, we held co-creation sessions, resulting in several designs of innovative mobility aids, which were evaluated for acceptance via nine in-depth interviews. <i>Results</i>: The interviews resulted in a myriad of key values, such as ‘independence from family’ and ‘doing their own groceries’. Design sessions resulted in three designs for a wheeled walker. Their acceptance was rather low. Current mobility device users were more eager to accept the designs than non-users. <i>Conclusion</i>: The value-based approach offers designers a close look into the lives of the elderly, thereby opening up a wide range of innovation possibilities that better fit their actual needs. Product service systems seem to be a promising focus for targeting human needs in mobility device design.
DOI: 10.21223/3buq5b/dsm04c
title: c51046e2ef2d49f5e90f950cc5ee3943.pdf
DOI: 10.21223/3buq5b/vahepb
title: 13419_DataDictionary_UniqueIdentifier.xlsx
DOI: 10.21223/3buq5b
title: Dataset for: RTBfoods K.1.5- Description of existing spectral database for Dried Milled Sweetpotato at CIP, Peru, Ghana, Mozambique, Uganda

description: This dataset contains the description of existing spectral database for dried milled sweetpotato at the International Potato Center (CIP), Peru, Ghana, Mozambique and Uganda.
DOI: 10.6084/m9.figshare.3619713
title: AOSLO single-cone color naming data files

description: Mosaic files contain xy location values and cone types of a mosaic of 600-800 cones. Cone types are encoded as follows: L=3, M=2, S=1.<br>Single cone color naming data collected on a white or blue background are collected in the other files for subjects 10001 and 20076. <br>Each row corresponds to a targeted cone. The x,y locations of that cone in the mosaic are recorded in the first two columns. Third column = cone type. The fourth column is the ID of that cone in the corresponding mosaic file. <br>Each cone was targeted at least 20 times. After each flash (500 ms; 543 nm), the subject reported the appearance of the flash with a single button press. The subject described the flash as: not seen (n.s.), white, red, green, blue or yellow. The number of times the button corresponding to each response was observed for each cone is tallied in the column corresponding to that category.<br><br>Relative to the white condition that produces roughly equal activation across the three cone types, the blue background <i>increased</i> the quantal catch of S-cones by ~134%. L- and M-cones were altered less so: activity <i>decreased</i> by ~19% and ~7% in L- and M-cones, respectively.<br><br>
DOI: 10.6084/m9.figshare.3615816
title: Data and code to reproduce Dallas and Drake 2016 "Fluctuating temperatures alter environmental pathogen transmission in aDaphnia-pathogen system"

description: This repository contains data files and R code (in an Rmd document) to reproduce analysis from a manuscript entitled "Fluctuating temperatures alter environmental pathogen transmission in a <i>Daphnia</i>-pathogen system" by Dallas and Drake to published in <i>Ecology and Evolution. <br><br> </i>The `Rmd` file can be rendered using the command<br><br> rmarkdown::render('tempVar.Rmd')<br><br>from within the directory containing the Rmd file. Errors will occur if the dependencies have not been satisfied (a few packages are used throughout). <br>
DOI: 10.6084/m9.figshare.3615816.v1
title: Data and code to reproduce Dallas and Drake 2016 "Fluctuating temperatures alter environmental pathogen transmission in aDaphnia-pathogen system"

description: This repository contains data files and R code (in an Rmd document) to reproduce analysis from a manuscript entitled "Fluctuating temperatures alter environmental pathogen transmission in a <i>Daphnia</i>-pathogen system" by Dallas and Drake to published in <i>Ecology and Evolution. <br><br> </i>The `Rmd` file can be rendered using the command<br><br> rmarkdown::render('tempVar.Rmd')<br><br>from within the directory containing the Rmd file. Errors will occur if the dependencies have not been satisfied (a few packages are used throughout). <br>
DOI: 10.6084/m9.figshare.3614595
title: data_and_documents_BMCOHealth

description: Additional data to be consulted by intrested readers<br>Paper: <br><pre>OHEA-D-16-00108R2 Comparison of occlusal caries detection using the ICDAS criteria on extracted teeth or their photographs Peter Bottenberg, PhD; Wolfgang Jacquet, PhD, MSc; Carolin Behrens; Vitus Stachniss, PhD; Anahita Jablonski-Momeni, PhD BMC Oral Health</pre>
DOI: 10.6084/m9.figshare.3614595.v1
title: data_and_documents_BMCOHealth

description: Additional data to be consulted by intrested readers<br>Paper: <br><pre>OHEA-D-16-00108R2 Comparison of occlusal caries detection using the ICDAS criteria on extracted teeth or their photographs Peter Bottenberg, PhD; Wolfgang Jacquet, PhD, MSc; Carolin Behrens; Vitus Stachniss, PhD; Anahita Jablonski-Momeni, PhD BMC Oral Health</pre>
DOI: 10.6084/m9.figshare.3607251
title: Activation of autophagy by FOXO3 regulates redox homeostasis during osteogenic differentiation

description: Bone remodeling is a continuous physiological process that requires constant generation of new osteoblasts from mesenchymal stem cells (MSCs). Differentiation of MSCs to osteoblast requires a metabolic switch from glycolysis to increased mitochondrial respiration to ensure the sufficient energy supply to complete this process. As a consequence of this increased mitochondrial metabolism, the levels of endogenous reactive oxygen species (ROS) rise. In the current study we analyzed the role of forkhead box O3 (FOXO3) in the control of ROS levels in human MSCs (hMSCs) during osteogenic differentiation. Treatment of hMSCs with H<sub>2</sub>O<sub>2</sub> induced FOXO3 phosphorylation at Ser294 and nuclear translocation. This ROS-mediated activation of FOXO3 was dependent on mitogen-activated protein kinase 8 (MAPK8/JNK) activity. Upon FOXO3 downregulation, osteoblastic differentiation was impaired and hMSCs lost their ability to control elevated ROS levels. Our results also demonstrate that in response to elevated ROS levels, FOXO3 induces autophagy in hMSCs. In line with this, impairment of autophagy by autophagy-related 7 (ATG7) knockdown resulted in a reduced capacity of hMSCs to regulate elevated ROS levels, together with a reduced osteoblast differentiation. Taken together our findings are consistent with a model where in hMSCs, FOXO3 is required to induce autophagy and thereby reduce elevated ROS levels resulting from the increased mitochondrial respiration during osteoblast differentiation. These new molecular insights provide an important contribution to our better understanding of bone physiology.
DOI: 10.6084/m9.figshare.3607251.v1
title: Activation of autophagy by FOXO3 regulates redox homeostasis during osteogenic differentiation

description: Bone remodeling is a continuous physiological process that requires constant generation of new osteoblasts from mesenchymal stem cells (MSCs). Differentiation of MSCs to osteoblast requires a metabolic switch from glycolysis to increased mitochondrial respiration to ensure the sufficient energy supply to complete this process. As a consequence of this increased mitochondrial metabolism, the levels of endogenous reactive oxygen species (ROS) rise. In the current study we analyzed the role of forkhead box O3 (FOXO3) in the control of ROS levels in human MSCs (hMSCs) during osteogenic differentiation. Treatment of hMSCs with H<sub>2</sub>O<sub>2</sub> induced FOXO3 phosphorylation at Ser294 and nuclear translocation. This ROS-mediated activation of FOXO3 was dependent on mitogen-activated protein kinase 8 (MAPK8/JNK) activity. Upon FOXO3 downregulation, osteoblastic differentiation was impaired and hMSCs lost their ability to control elevated ROS levels. Our results also demonstrate that in response to elevated ROS levels, FOXO3 induces autophagy in hMSCs. In line with this, impairment of autophagy by autophagy-related 7 (ATG7) knockdown resulted in a reduced capacity of hMSCs to regulate elevated ROS levels, together with a reduced osteoblast differentiation. Taken together our findings are consistent with a model where in hMSCs, FOXO3 is required to induce autophagy and thereby reduce elevated ROS levels resulting from the increased mitochondrial respiration during osteoblast differentiation. These new molecular insights provide an important contribution to our better understanding of bone physiology.
DOI: 10.6084/m9.figshare.3606753
title: Association of frequent genetic variants in platelet activation pathway genes with large-vessel ischemic stroke in Polish population

description: Platelets are critically involved in the development of cerebral ischemia. Our study aimed to establish an association between frequent (minor allele frequency (MAF) &gt; 5%) genetic polymorphisms in 84 candidate genetic loci previously linked to platelet reactivity by the use of next-generation sequencing of exons from pooled DNA samples in Polish patients with a history of large-vessel ischemic stroke. Genetic analysis was performed on blood samples obtained from 500 patients (diagnosed with acute non-cardioembolic ischemic stroke with coexisting large-artery atherosclerosis) and age/sex/history of smoking matching 500 controls of Polish origin with high risk of cardiovascular disease. Sequencing of 10 pools (five for each ischemic and control groups) was performed on the Ilumina HiSeq2500 sequencer which generated an average of 36.1 (22.7–45.9 range) million pair-end 101 bp reads and 5.3 (3–7 range) Gbp per pooled sample consisting of 100 subjects. In total, we observed 789 frequent polymorphisms in the sequenced 84 genes (703 of single-nucleotide polymorphism (SNP) type and 86 indels). When the MAF between control and stroke groups was compared, only two intronic polymorphisms (1 SNP and 1 indel) in <i>RGS7</i> (rs127445 36) and <i>ANKS1B</i> (rs398098426) genes, respectively, show statistically significant differences, which persisted after individual genotyping of the variants and adjustment for potential confounding factors. From the remaining variants, 35 polymorphisms displayed various degrees of nominal significance (from 0.6.3 × 10<sup>−5</sup> to 5 × 10<sup>−2</sup>) and 754 polymorphisms did not show any statistical significance when comparison was evaluated for differences in MAF between the study groups. In conclusion, the results of the study demonstrate statistically significant differences in two frequent intronic genetic variants (in <i>RGS7</i> and <i>ANKS1B</i>) that could be associated with the platelet function between ischemic stroke patients with coexisting large-vessel atherosclerosis and control patients having high vascular risk.
DOI: 10.6084/m9.figshare.3606753.v1
title: Association of frequent genetic variants in platelet activation pathway genes with large-vessel ischemic stroke in Polish population

description: Platelets are critically involved in the development of cerebral ischemia. Our study aimed to establish an association between frequent (minor allele frequency (MAF) &gt; 5%) genetic polymorphisms in 84 candidate genetic loci previously linked to platelet reactivity by the use of next-generation sequencing of exons from pooled DNA samples in Polish patients with a history of large-vessel ischemic stroke. Genetic analysis was performed on blood samples obtained from 500 patients (diagnosed with acute non-cardioembolic ischemic stroke with coexisting large-artery atherosclerosis) and age/sex/history of smoking matching 500 controls of Polish origin with high risk of cardiovascular disease. Sequencing of 10 pools (five for each ischemic and control groups) was performed on the Ilumina HiSeq2500 sequencer which generated an average of 36.1 (22.7–45.9 range) million pair-end 101 bp reads and 5.3 (3–7 range) Gbp per pooled sample consisting of 100 subjects. In total, we observed 789 frequent polymorphisms in the sequenced 84 genes (703 of single-nucleotide polymorphism (SNP) type and 86 indels). When the MAF between control and stroke groups was compared, only two intronic polymorphisms (1 SNP and 1 indel) in <i>RGS7</i> (rs127445 36) and <i>ANKS1B</i> (rs398098426) genes, respectively, show statistically significant differences, which persisted after individual genotyping of the variants and adjustment for potential confounding factors. From the remaining variants, 35 polymorphisms displayed various degrees of nominal significance (from 0.6.3 × 10<sup>−5</sup> to 5 × 10<sup>−2</sup>) and 754 polymorphisms did not show any statistical significance when comparison was evaluated for differences in MAF between the study groups. In conclusion, the results of the study demonstrate statistically significant differences in two frequent intronic genetic variants (in <i>RGS7</i> and <i>ANKS1B</i>) that could be associated with the platelet function between ischemic stroke patients with coexisting large-vessel atherosclerosis and control patients having high vascular risk.
DOI: 10.6084/m9.figshare.3603768
title: Toxicity of microplastic particles and adsorbed fluoranthene to epibenthic copepods

description: This study focuses on the chronic uptake of polyethylene microplastics in the gut of the epibenthic copepod, <i>Amphiascus tenuiremis</i>, for a period of 32 days. Uptake is quantified during the three developmental stages, and the resulting growth, reproduction, and mortality is quantified. Polyethylene microplastics used in this study ranged between 10 and 27 microns. The same polyethylene microplastics were saturated with the polycyclic aromatic hydrocarbon, fluoranthene, and the bioavailability of fluoranthene to adult copepods was also quantified following 7 day exposures.<br>
DOI: 10.6084/m9.figshare.3602976
title: Read all about it!? Public accountability, fragmented global climate governance and the media

description: The way that the media reports and comments on key events in the fragmented global climate governance landscape is one important route to strengthening public accountability of such governance. Editorials and other opinion pieces provide key contributions to the public sphere, but have been almost entirely neglected in media research on climate change. Another understudied aspect in such research is the reporting on the fragmentation of global climate governance across numerous forums. This article provides an exploratory approach to address these two research gaps. It presents a quantitative analysis of how often leading newspapers in seven countries (Finland, India, Laos, Norway, South Africa, UK and USA) wrote about 18 meetings in six different global climate governance forums between 2004–2009 and whether they provided commentaries about them. The study shows that media coverage (articles and opinion pieces) is limited or absent for many meetings that are not attended by heads of state, are the launch of a new process or do not have the convening power of the United Nations Framework Convention on Climate Change. The pattern of coverage differs significantly among individual newspapers and there is no clear distinction between developed and developing country newspapers. The article concludes that overall news coverage, and editorial commentary in particular, of global climate meetings in the selected newspapers is too low and too patchy to significantly support domestic publics to hold their own (and indirectly other) governments accountable with regard to fragmented global climate governance. <b>Policy relevance</b> This study is instructive for the media and civil society, who should both act as accountholders of governments with regard to how they act in global climate governance and its implementation. Reporting and commentaries need to reflect the overarching process, not only sporadic coverage of high-level meetings, but also critical analysis of what is achieved. They should also take a broader scope in terms of the kinds of meetings and processes in global governance that they cover. Civil society should encourage the media to increase coverage along these lines, e.g. by adequate monitoring of government actions (or lack thereof) and share this with the media.