cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/mygcb-x2b32
title: MEF cells with FCCP

description: To study mitochondrial division. Grid - WY43_grid3.
description: <strong>Tilt Series Date:</strong> 2018-07-29</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -2.5 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD and tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze grids. Shrawan FIB-milled cells and performed ECT. Cells courtesy of Chan lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were treated for 3 min with 10 µM FCCP before plunge freezing and FIB-milling.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-29-6/rawdata/</p>TS6.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-29-6/rawdata/TS6.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/60j99-10g33
title: HeLa (Vps4B KD) - PM damaged

description: HeLa (Kyoto line stably expressing CHMP4B-EGFP at low levels; courtesy of MPI) knocked down for Vps4B (double transfection) and laser damaged. Grid - WY54_grid1.
description: <strong>Tilt Series Date:</strong> 2018-07-08</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, transfected with siRNA and caused plasma membrane damage using Laser before Shrawan froze the grids and performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were knocked down for Vps4B with SMART pool siRNA for 48 hr (2 x 24 hr). Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-08-9/rawdata/</p>TS15.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-08-9/rawdata/TS15.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rh091-avh74
title: BacillusSubtilis_328_4hr

description: Bacillus subtitles strain 328 overexpressing ymdB.
description: <strong>Tilt Series Date:</strong> 2020-02-26</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB328, ΔponA ΔymdB amyE::ymdB Δhag</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Samples were prepared with Ritesh and Amit from Ilan Rosenshine and Sigal ben Yehuda labs at . the Hebrew university. See tomograms mka2020-02-08-x.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were prepared as mentioned in Bacillus subtitles tomograms taken on 8th of February . 2020.</p>
<strong>Sample Preparation:</strong> Samples were prepared as mentioned in Bacillus subtitles tomograms taken on 8th of February . 2020. See tomograms mka2020-02-08-x.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-26-34/rawdata/</p>Bsubtilis_328_016.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4g7vm-wyb78
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, stationary phase
description: <strong>Tilt Series Date:</strong> 2019-11-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> stationary phase, K3 detector with CDS mode</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, blot force 6, blot time 3s, drain time 1s,  wait time 0s. 4uL of 6X diluted cell (original O.D. 01.2). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-15-69/rawdata/</p>20191115_Bd_deltaH_ST_33.mrc,  Tilt Series (Pixel Size 2.678 nm), 11.1 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mf1sb-dq250
title: Shewanella oneidensis MR-1

description: Raw data files of Shewanella oneidensis MR-1
description: <strong>Tilt Series Date:</strong> 2018-05-22</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were prepared in chemostat (2 days) by Sahand from Moh's group in USC.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> These are fixed cells prepared in chemostat (2 days). Poorna has prepared these grids but they were not good (only few cells present per grid). I imaged them on the Polara to check if they have "iron cores" so that I send them to Sharon in Weizmann Institute.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-22-12/rawdata/</p>sq2mont.mrc,  Tilt Series (Pixel Size 0.39 nm), 256.0 MB  
        
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DOI: 10.22002/ycxxn-zf820
title: mini cells

description: Mini cells (FtsA FtsZ over-expression)
description: <strong>Tilt Series Date:</strong> 2019-11-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Strain from Craig Roy lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> FtsA FtsZ over-expression induced by 0.1mM IPTG in liquid culture. O.D. reached 1.8 after ~40 hours. </p>
<strong>Sample Preparation:</strong> Concentrate 600uL of O.D. 1.8 cells, collect supernatant from 500Xg 2min spin, then concentrate again w/ 1000xg 5min spin. Resuspend pellet to 50uL as stock. Vitrobot set to 22 C, 100% humidity. Apply 4uL of 20X diluted stock, blot manually. Quantifoil R2/2 copper.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-15-45/rawdata/</p>20191115_Lpmini_63.mrc,  Tilt Series (Pixel Size 0.1339 nm), 7.2 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.vavy7b
title: Occurrence Download

description: A dataset containing 21 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorghum arundinaceum",
 "Country is Papua New Guinea",
 "HasCoordinate is true"
 ]
}

 The dataset includes 21 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014148-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.4s65q5
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Carduus acanthoides"
 ]
}

 The dataset includes 2 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014150-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.cggrnm
title: Occurrence Download

description: A dataset containing 4 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorghum arundinaceum",
 "Country is Solomon Islands",
 "HasCoordinate is true"
 ]
}

 The dataset includes 4 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014151-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.y7vnd3
title: Occurrence Download

description: A dataset containing 117 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Trachydoras paraguayensis)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 117 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014147-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/yg5sb-etb22
title: AD348_delVirB_Agro

description: Mutant Agro cells, delVirB (whole of the VirB system deleted). Grown on induction plate pH5.8 in exactly similar way as the AD1484 strain.
description: <strong>Tilt Series Date:</strong> 2016-01-06</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow at 19C for 4 days on induction plate. Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD348_delVirB) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  dga2016-01-06-5_slicer19969.jpg: Filaments in the cell?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-06-5/rawdata/</p>AD348_delVirB0005.mrc,  Tilt Series (Pixel Size 4.8 nm), 3.4 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mea7s-c6g28
title: Thermococcus koakarensis

description: Imaging Thermococcus koakarensis flagella
description: <strong>Tilt Series Date:</strong> 2016-09-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard Tk medium with starch and sulfur</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-19-2/rawdata/</p>atlas10003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-19-2/rawdata/atlas10003.mrc"
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DOI: 10.22002/9ya16-5j103
title: Exp phase S. acidocaldarius

description: To study the division belt.
description: <strong>Tilt Series Date:</strong> 2017-12-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD. (assuming same conditions as Dec 21 batch)</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually. (assuming same conditions as Dec 21 batch)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-05-4/rawdata/</p>TS4.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.ex7h68
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Sorghum arundinaceum",
 "Country is Samoa",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0014149-250827131500795/datasets/export for details.
DOI: 10.22002/2mtwk-nqh88
title: S. acidocaldarius

description: Raw data files of S. acidocaldarius
description: <strong>Tilt Series Date:</strong> 2017-11-22</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Megan's cell stock</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells in ATCC 1723 medium at 76 C to late log or stationary phase, constantly shaking at >220 rpm and topping up H2O that evaporates (every 12-16 hrs).</p>
<strong>Sample Preparation:</strong> Resuspend cells to ~5-10 OD in the same medium. Freeze 3.5 µL of cells in Cu finder grids by blotting manually from behind. A very aggressive blotting technique leads to dry cells. Some frozen grids were FIB milled after Pt coating (2 mA for 10 sec) and some frozen grids were coated with 3-5 nm Au beads in 2-methyl butane (washed later with Ethane/Propane)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-11-22-14/rawdata/</p>TS6.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.1 GB  
        
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DOI: 10.22002/9s135-37n25
title: Exp phase S. acidocaldarius

description: To study the division belt.
description: <strong>Tilt Series Date:</strong> 2017-12-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD. (assuming same conditions as Dec 21 batch)</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually. (assuming same conditions as Dec 21 batch)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-05-5/rawdata/</p>TS5.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-05-5/rawdata/TS5.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j5j02-05z34
title: Exp phase S. acidocaldarius

description: To study the division belt.
description: <strong>Tilt Series Date:</strong> 2017-12-05</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD. (assuming same conditions as Dec 21 batch)</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually. (assuming same conditions as Dec 21 batch)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-05-9/rawdata/</p>TS9.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
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