DOI: 10.5061/dryad.vq6m6
title: Data from: Tropical Drosophila pandora carry Wolbachia infections causing cytoplasmic incompatibility or male killing

description: Wolbachia infections have been described in several Drosophila species,
 but relatively few have been assessed for phenotypic effects. Cytoplasmic
 incompatibility (CI) is the most common phenotypic effect that has been
 detected, while some infections cause male killing or feminization, and
 many Wolbachia infections have few host effects. Here, we describe two new
 infections in a recently described species, Drosophila pandora, one of
 which causes near-complete CI and near-perfect maternal transmission (the
 “CI” strain). The other infection is a male killer (the “MK” strain),
 which we confirm by observing reinitiation of male production following
 tetracycline treatment. No incompatibility was detected in crosses between
 CI strain males and MK strain females, and rare MK males do not cause CI.
 Molecular analyses indicate that the CI and MK infections are distantly
 related and the CI infection is closely related to the wRi infection of
 Drosophila simulans. Two population surveys indicate that all individuals
 are infected with Wolbachia, but the MK infection is uncommon. Given
 patterns of incompatibility among the strains, the infection dynamics is
 expected to be governed by the relative fitness of the females, suggesting
 that the CI infection should have a higher fitness. This was evidenced by
 changes in infection frequencies and sex ratios in population cages
 initiated at different starting frequencies of the infections.
description: CI strain_Cytoplasmic
 IncompatibilityLab collected crossing
 data for CI strain of Drosophila pandora, used to evaluate Cytoplasmic
 Incompatibility. Data from two experiments (Jan 2014 and April 2014),
 showing the number of hatched/unhatched eggs collected and scored, and the
 number of male and female progeny collected and scored. Crosses where no
 mating occurred excluded for analysis but identified here for
 completeness.MK
 strain_CrossesLab collected crossing
 data for MK strain of Drosophila pandora, used to evaluate Cytoplasmic
 Incompatibility where CI68+ is the MK strain. Data showing the number of
 hatched/unhatched eggs collected and scored, and the number of male and
 female progeny collected and scored. Crosses where no mating occurred
 excluded for analysis but identified here for
 completeness.MK strain_28 Degree
 AgeLab collected data
 evaluating female age and progeny sex ratio for MK strain of Drosophila
 pandora (strain C168+). Data shows the number of male and female progeny
 collected and scored when females at 28 degrees.MK strain_28 Degree Male
 CrossesLab collected data for
 crosses between the CI and MK strains of Drosophila pandora, using males
 reared at 28 degrees (and including rare males from the MK line). Data
 shows the number of hatched/unhatched eggs collected and scored, and the
 number of male and female progeny collected and scored. Crosses where no
 mating occurred excluded for analysis but identified here for
 completeness.CI and MK
 strains_FecundityLab collected data for
 CI and MK strains of Drosophila pandora, used to evaluate Fecundity. Data
 showing the number of eggs collected. Crosses where no mating occurred
 excluded for analysis but identified here for
 completeness.CI and MK
 strains_Population Expt_Sex Ratio ScoreLab collected data for
 population monitoring experiment with different starting frequencies of
 the MK strain of Drosophila pandora (10, 25, 50, 75 and 90%). Data shows
 the number of females and males in a random sample of 100 progeny from
 each replicate after five generations.Treatment refers to the starting
 frequency of the MK infection.CI and MK
 strains_Population Expt_Infection ScoreLab collected data for
 population monitoring experiment with different starting frequencies MK
 strain of Drosophila pandora (10, 25, 50, 75 and 90%). Data shows the
 infection type of a random sample of 50 females from each replicate after
 five generations. Treatment is the starting frequency of the MK strain.
 The MK infection is scored as '1' and the CI infection is scored
 as '0'.CI strain_Maternal
 TransmissionLaboratory data for the
 CI strain of Drosophila pandora showing the relationship between maternal
 transmission of Wolbachia and egg hatch rate, where a high egg hatch rate
 corresponds with imperfect Wolbachia transmission. Crosses for which
 mating did not occur were excluded from analysis, but are included here
 for completeness.