cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.21430/m3j2pt6aor
title: Genotype Polymorphism effects on Cholera Vaccine responses.

description: Innate immunity gene products are known to be the first-line of host-defense and to control and assist the adaptive immune system. This research represents the opportunity to study, in detail, the responses longitudinally to vaccination (using clinical examination and laboratory assays) in a large ethnic population. Consequently, a prioritized set of genes and gene products from both systems will be evaluated.
DOI: 10.21430/m3j7z67hea
title: Determinants of human NK cell diversity by mass cytometry

description: Natural Killer (NK) cells play critical roles in immune defense and reproduction, yet remain the most poorly understood lymphocyte population. Because their activation is controlled by a variety of combinatorially expressed activating and inhibitory receptors, NK cell diversity and function are closely linked. To provide an unprecedented understanding of NK cell repertoire diversity, we used mass cytometry to simultaneously analyze 35 parameters, including 28 NK cell receptors, on peripheral blood NK cells from five sets of monozygotic twins and twelve unrelated donors of defined HLA and killer cell immunoglobulin-like receptor (KIR) genotype. This analysis revealed a remarkable degree of NK cell diversity, with an estimated 6,000-30,000 phenotypic populations within an individual and &gt;100,000 phenotypes in this population. Genetics largely determined inhibitory receptor expression, whereas activation receptor expression was heavily environmentally influenced. Therefore, NK cells may maintain self-tolerance through strictly regulated expression of inhibitory receptors, while using adaptable expression patterns of activating and costimulatory receptors to respond to pathogens and tumors. These findings further suggest the possibility that discrete NK cell subpopulations could be harnessed for immunotherapeutic strategies in the settings of infection, reproduction, and transplantation
DOI: 10.21430/m3k0gcb4kl
title: Role of Antimicrobial Peptides in Host Defense Against Vaccinia Virus (AMP 01)

description: Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by recurrent viral skin infections. Recent studies have demonstrated that the skin of people with AD my have decreased antimicrobial peptide (AMP) expression. The purpose of this study is to compare small pox virus replication and the number of AMPs and other antiviral molecules in people with AD, as compared to those seen in people with psoriasis or asthma, or healthy individuals.
DOI: 10.21430/m3kdrikjob
title: Determine whether differences in TLR-responsiveness alter susceptibility to Biodefense priority pathogens using WN virus as a paradigm or responsiveness to vaccination using influenza as a model shared to Semi-Public Workspace (SPW) Project

description: To assess the effects of the impairments in innate immunity identified in the study titled <i>Delineate innate immune responses in populations at risk (the elderly and the immunosuppressed) that are different from the general population</i> on the efficiency of the response to agents of Bioterrorism, using WN virus infection and influenza vaccination as models.<br><br>Will also assess these responses directly from patients who had WN virus infection or the influenza vaccine. Will elucidate mechanisms mediating innate immune responses to viral infection using murine models.
DOI: 10.21430/m3kedfbdq7
title: Comparison of immune response to influenza vaccine in transplant patients and healthy controls

description: To determine the effects of chronic immunosuppressive therapies on the magnitude and character of the adaptive immune response to influenza vaccination.
DOI: 10.21430/m3kg2xm196
title: Study to measure the immune response to the influenza vaccine in patients with chronic plaque psoriasis

description: Interleukin-12 (IL-12) and interleukin-23 are heterodimeric cytokines, with a common p40 subunit and a unique chain (IL-12p35 and IL-23p19). The p40 subunit of both interleukins binds to the transmembrane IL-12 receptor-beta1 (IL-12R ) on the surface of T lymphocytes and natural killer cells. Ustekinumab is a fully human monoclonal antibody, anti-IL12p40, which binds to the p40 subunit of IL-12 and IL-23 with high affinity and specificity, inhibiting the activity of both interleukins. Ustekinumab has proven to be highly effective in the treatment of chronic plaque psoriasis, with up to 76% of patients achieving a 75% reduction in their psoriasis area and severity index (PASI-75).1, It is routinely recommended that psoriasis patients treated with biologic therapies such as ustekinumab be vaccinated annually with the influenza vaccine. We can thus assess the importance of these cytokines in the immune response to vaccination by comparing the immune response to influenza vaccination of psoriasis patients treated with ustekinumab with that of patients who are not receiving this treatment. We will assess gene expression profiles and white blood cell subsets in the blood of psoriasis patients before vaccination and at multiple time-points after vaccination.
DOI: 10.21430/m3khtsssn7
title: Plasmablast response to inactivated and live attenuated influenza vaccines (TIV3/TIV3 ID) in 2013

description: To study the plasmablast response to 2012 seasonal inactivated infuenza vaccine.
DOI: 10.21430/m3kiqa4lov
title: The role of TLR4 and TRIF signaling in TH2 response to papain

description: The role of TLR4 signaling in TH2 response to papain was measured by IL-4 and antibody production. The involvement of MyD88, TRIF, and oxidized phospholipids in the TLR4 pathway was examined. Basophil recruitment to the draining lymph node through dendritic-cell-secreted CCL7 was investigated as a component of the TLR4/TRIF pathway.
DOI: 10.21430/m3klk90p9t
title: Analysis and Correlation of Cathelicidin Expression in Skin and Saliva of Subjects with Atopic Dermatitis and Psoriasis (CATH 02)

description: Cathelicidins are small proteins in the human body that protect against infection. The purpose of this study is to determine if the amount of cathelicidins and other small proteins found in saliva can predict the amount of these in the skin of people who have acute atopic dermatitis (AD) or psoriasis.
DOI: 10.21430/m3kpfs7kxi
title: Plasmablast response to inactivated and live attenuated influenza vaccines (TIV3/TIV3 ID/LAIV) in 2011

description: To study the plasmablast response to influenza vaccines
DOI: 10.21430/m3kphzf88e
title: MicroRNA Regulation of Human Protease Genes

description: the human protease genes required for influenza virus replication were determined and validated using RNA interference approaches. The genes validated as critical for influenza virus replication were ADAMTS7, CPE, DPP3, MST1, and PRSS12, and pathway analysis showed these genes were in global host cell pathways governing inflammation (NF-kB), cAMP/calcium signaling (CRE/CREB), and apoptosis.
DOI: 10.21430/m3larlt608
title: Analysis of the Response of Subjects with Atopic Dermatitis to Oral Vitamin D3 by Measurement of Antimicrobial Peptide Expression in Skin and Saliva (CATH 03)

description: The goal of the Atopic Dermatitis Vaccinia Network (ADVN) is to research methods for preventing atopic dermatitis (AD) patients from contracting eczema vaccinatum (EV), a potentially fatal complication of smallpox vaccinations. A critical host defense defect uncovered in patients with AD is their apparent relative lack of expression of antimicrobial peptides (AMPs), specifically cathelicidins, under inflammatory conditions. AMPs are important effectors and triggers in the innate immune system, and the lack of expression in AD patients could be a key component in their susceptibility to EV. This study will examine whether or not administration of oral Vitamin D3 given over 21 days will change the AMP expression in the skin or saliva of AD subjects and healthy controls.
DOI: 10.21430/m3lgj7ddzn
title: Human Elispot Assay with peptide.

description: Human Elispot Assay with peptide, Influenza A epitope reactivity on HLA-A02 positive random donors.
DOI: 10.21430/m3ls2srm8m
title: Heterovariant cross-reactive B-cell responses induced by the 2009 pandemic influenza virus A subtype H1N1 vaccine

description: To study the plasmablast response to monovalent inactivated 2009 pandemic pH1N1 vaccine
DOI: 10.21430/m3nq3uf8o9
title: Impact of Nutrition and Nitazoxanide on Enteroaggregative Escherichia coli (EAEC) Infection

description: Comparing EAEC JM221 to EAEC 042; comparing infection in nourished vs malnourished mice; studying the effect of NTZ on EAEC 042 infection
DOI: 10.21430/m3nwmduz08
title: Heterosubtypic Influenza infection antagonizes elicitation of immunological reactivity to hemagglutinin

description: Influenza-specific immunity in humans is unique because there are repeated exposures to viral strains containing genetically conserved epitopes recruiting memory CD4 T cells and novel epitopes stimulating naive CD4 T cells, possibly resulting in competition between memory and naive lymphocytes. In this study, we evaluated the effect of this competition on CD4 T cell and B cell response specificity using a murine model of sequential influenza infection. We found striking and selective decreases in CD4 T cell reactivity to nonconserved hemagglutinin (HA) epitopes following secondary influenza infection. Surprisingly, this shift in CD4 T cell specificity was associated with dramatic decreases in HA-specific Ab. These results suggest that repeated exposure to influenza viruses and vaccines containing conserved internal proteins may have unintended and negative consequences on the ability to induce HA-specific Ab to novel pandemic strains of influenza. These finding could have important implications on pandemic influenza preparedness strategies.
DOI: 10.21430/m3odyabdl2
title: VZV vaccination in the elderly

description: Healthy adults (50+ years old) with history of varicella but no history of zoster are vaccinated with Zostavax. Blood and serum are taken prior to vaccination and at several points after. A systems biology approach will be used to identify age-related decreases in immune function and potential predictors and correlates of protection.
DOI: 10.21430/m3ol4zg9jn
title: Determining the effect of different adjuvants and doses on the immune response to rPA

description: Inbred C57BL/6 female mice (12-15 weeks old) were immunized subcutaneously according to the 7 immunization groups. Serum samples were collected weekly from subgroup A and analyzed serum antibody response using ELISA, Toxin Neutralization Assays, and Surface Plasmon Resonance. Also, brachial and inguinal lymph nodes were harvested from 3 of each group at 10 time points. Lymph nodes from one side of each mouse were used for histology and nodes from the other side were used for phenotyping by flow cytometry.
DOI: 10.21430/m3om5t92k5
title: The peptide specificity of the endogenous T follicular helper cell repertoire generated after protein immunization

description: T follicular helper (Tfh) cells potentiate high-affinity, class-switched antibody responses, the predominant correlate of protection from vaccines. Despite intense interest in understanding both the generation and effector functions of this lineage, little is known about the epitope specificity of Tfh cells generated during polyclonal responses. To date, studies of peptide-specific Tfh cells have relied on either the transfer of TcR transgenic cells or use of peptide:MHC class II tetramers and antibodies to stain TcR and follow limited peptide specificities. In order to comprehensively evaluate polyclonal responses generated from the natural endogenous TcR repertoire, we developed a sorting strategy to separate Tfh cells from non-Tfh cells and found that their epitope-specific responses could be tracked with cytokine-specific ELISPOT assays. The immunodominance hierarchies of Tfh and non-Tfh cells generated in response to immunization with several unrelated protein antigens were remarkably similar. Additionally, increasing the kinetic stability of peptide-MHC class II complexes enhanced the priming of both Tfh and conventional CD4 T cells. These findings may provide us with a strategy to rationally and selectively modulate epitope-specific Tfh responses. By understanding the parameters that control epitope-specific priming, vaccines may be tailored to enhance or focus Tfh responses to facilitate optimal B cell responses.
DOI: 10.21430/m3p4jjnfs2
title: Primary seasonal influenza virus infection elicits CD4 T cells specific for genetically conserved epitopes that can be mobilized for cross protective immunity to pandemic H1N1 influenza

description: In this study, a mouse model of primary and secondary influenza infection is developed by using a widely circulating seasonal H1N1 virus and the pandemic strain of H1N1 that emerged in Mexico in 2009, and several key issues are evaluated. The specificity of CD4 T cell reactivity toward epitopes conserved among H1N1 viruses or unique to the seasonal or pandemic strain was assessed by ELISpot assays. It was observed that a secondary challenge with the pandemic virus was associated with rapid expansion of memory CD4 T cells specific for shared determinants, decreased viral titers and accelerated neutralizing anti-influenza antibody responses to the serologically distant novel pandemic strain.
DOI: 10.21430/m3p8mvpekb
title: Original Antigenic Sin Responses to Influenza Viruses

description: Determining the extent to which original antigenic sin is induced by variant influenza viruses by using two related strains of influenza
DOI: 10.21430/m3phw2d08d
title: CD4 T cell response to pH1N1 vaccination

description: Healthy adult human subjects were given monovalent inactivated pH1N1 vaccine and antibody responses were assessed using HAI and MN assays. CD4 T cell reactivity was examined using IFNg Elispot assays to look at reactivity to unique and conserved peptide pools.
DOI: 10.21430/m3q2o0x9z5
title: Oral Immunotherapy for Childhood Egg Allergy (CoFAR3)

description: The purpose of this study is to determine the safety and efficacy of the administration of oral immunotherapy (OIT). The intent is to develop desensitization and eventually tolerance to egg allergen.
DOI: 10.21430/m3q6u5ggrt
title: Defining signatures for immune responsiveness by functional systems immunology

description: Project 2: Immunologic and genomic signatures of response to neuroinvasive or non-neuroinvasive West Nile Virus infection.
DOI: 10.21430/m3qjjf9a71
title: WISP-R ITN029ST: Withdrawal of Immunosuppression in Pediatric Liver Transplant Recipients

description: Antirejection medicines are prescribed to organ transplant recipients to prevent new organ rejection. Long-term use places transplant recipients at higher risk of serious infections and certain types of cancer. The purpose of this study is to determine whether immunosuppressive drugs can be safely withdrawn over a minimum of 9 months from children who received liver transplants at least 4 years ago.
DOI: 10.21430/m3qxqfgtk5
title: Immune response evaluation in patients treated with TNF-alpha blockade (anti-TNF)

description: A systematic study of human immune function with the goal of identifying defective mechanisms of immune response in patients treated with TNF-alpha blockade (anti-TNF)
DOI: 10.21430/m3qz1a9ooe
title: Miller-Burke ITN022ST: Using Donor Stem Cells and Alemtuzumab to Prevent Organ Rejection in Kidney Transplant Patients

description: This study will evaluate treatment of kidney transplant recipients with alemtuzumab and other immune system suppressing medications with or without infusions of bone marrow stem cells from the kidney donor. The purpose of this study is to find out which strategy is more effective in preventing organ rejection and maintaining patient health.
DOI: 10.21430/m3rdgzh60o
title: Monozygotic and Dizygotic Twin Pair T-Cell Responses to Influenza Vaccination 2010

description: Evaluate the variation in immune response between individuals and assess whether it changes as a function of age and similarity in genetic and environmental background (by comparing differences between monozygotic and dizygotic twin pairs of different ages).
DOI: 10.21430/m3re95ac35
title: Programming the magnitude and persistence of antibody responses with innate immunity

description: Excerpted experiment: Adjuvanting the 2009 pandemic H1N1 whole inactivated virus (WIV) with PLGA(MPL+R837) confers enhanced antigen-specific immunity in mice
DOI: 10.21430/m3rh4ft7xc
title: Vaccination with drifted variants of H5 hemagglutinin protein elicits a broadened antibody response

description: Assess the humoral response of mice immunized with drifted variant H5 hemagglutinin proteins
DOI: 10.21430/m3se0evy36
title: Aerosol Inoculation with a Sub-lethal Influenza Virus Leads to Exacerbated Morbidity and Pulmonary Disease Pathogenesis

description: Examined how the method of inoculation affected immunity and disease pathogenesis in mice using mouseadaptedH3N2 A/Aichi/2/68 (x31)
DOI: 10.21430/m3sfpacka3
title: LEAP ITN032AD: Induction of Tolerance through Early Introduction of Peanut in High-Risk Children, LEAP-On ITN049AD: The Persistence of Oral Tolerance Induction to Peanut and Its Immunological Basis

description: ITN032AD: This study will evaluate whether early exposure to peanuts promotes tolerance and provides protection from developing peanut allergy in children who are allergic to eggs or who have severe eczema. ITN049AD: This study will evaluate persistent tolerance to peanut by assessing the effect of 12 months of cessation of peanut consumption after 5 years of consumption versus continued avoidance of peanut.
DOI: 10.21430/m3sghw16wz
title: VRC304 - A Phase I Study of the Safety and Immunogenicity of a Recombinant DNA Plasmid Vaccine (VRC-AVIDNA036-00-VP) Encoding for the Influenza Virus H5 Hemagglutinin Protein in Healthy Adults

description: VRC304 - A Phase I, double-blind, placebo-controlled, randomized, dose escalation study to evaluate safety, tolerability, and immunogenicity of a recombinant DNA vaccine against the influenza virus hemagglutinin H5.
DOI: 10.21430/m3sig93pom
title: HLA Homozygous Cell Haplotype Sequencing

description: Assay development for HLA homozygous cell haplotype sequencing
DOI: 10.21430/m3sr2t3wzf
title: Self-recognition drives the preferential accumulation of promiscuous CD4+ T-cells in aged mice

description: T-cell recognition of self and foreign peptide antigens presented in MHC molecules (pMHC) is essential for life-long immunity. How the ability of the CD4+ T-cell compartment to bind self- and foreign-pMHC changes over the lifespan remains a fundamental aspect of T-cell biology that is largely unexplored. We report that, while old mice (18-22 months) contain fewer CD4+ T-cells compared with adults (8-12 weeks), those that remain have a higher intrinsic affinity for self-pMHC, as measured by CD5 expression. Old mice also have more cells that bind individual or multiple distinct foreign-pMHCs, and the fold increase in pMHC-binding populations is directly related to their CD5 levels. These data demonstrate that the CD4+ T-cell compartment preferentially accumulates promiscuous constituents with age as a consequence of higher affinity T-cell receptor interactions with self-pMHC.
DOI: 10.21430/m3stulgp9k
title: Responses to Immunization with Keyhole Limpet Hemocyanin (KLH) Administered by Scarification and the Intradermal (ID) Route (KLH 07)

description: Atopic dermatitis (AD) is a skin disorder in which people often have swelling and skin infections. People with this disease cannot receive the smallpox vaccine because it could cause them to have a fatal reaction known as eczema vaccinatum (EV). Keyhole limpet hemocyanin (KLH) is a protein that can be used to deliver vaccines to the body. The purpose of this study is to determine a baseline immune reaction to KLH in people without AD. Once this has been established, other studies can be designed to determine whether KLH can be used to give vaccines to people with AD.
DOI: 10.21430/m3t0bgmggc
title: Systems Biology Approach to Analysis of 2012-13 TIV Fluzone Influenza Vaccine Response in Healthy Individuals (see companion studies SDY74, SDY296)

description: This project will contribute to the overall vision and goals of this U19 by analyzing the immune response to Flu vaccination in healthy individuals. The knowledge generated in this Project will be transferred to Projects 3-5 where immune effects of vaccination will be studied in patients with underlying immune system alterations.
DOI: 10.21430/m3t5psba22
title: Effect of Influenza A (H5N1) Vaccine Prepandemic Priming on CD4+ T-Cell Responses

description: Introduction: Previous priming with avian influenza vaccines results in more rapid and more robust neutralizing antibody responses upon revaccination, but the role CD4+ T cells play in this process is not currently known.
DOI: 10.21430/m3ti5nz7vl
title: Immunologic and genomic signatures of response to Hepatitis C Virus infection.

description: Examine the immune response in primary immune cells from subjects who have spontaneously cleared HCV compared to HCV chronically infected subjects
DOI: 10.21430/m3tj6djley
title: Impact of immunosuppressive regimens on protective immunity in renal transplant recipients

description: A prospective longitudinal study to determine biological mechanisms that underlie the immunosuppressed state associated with immunosuppressive regimens after renal transplantation.
DOI: 10.21430/m3tk42r0qr
title: Rituximab for the Treatment of Wegener's Granulomatosis and Microscopic Polyangiitis (RAVE ITN021AI)

description: Antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis is the most common type of small blood vessel inflammation in adults. ANCA-associated vasculitis includes Wegener's granulomatosis (WG) and microscopic polyangiitis (MPA). Rituximab is a man-made antibody used to treat certain types of cancer. The purpose of this study is to determine the effectiveness of rituximab in treating patients with WG and MPA
DOI: 10.21430/m3tus7c3an
title: Safety in Immunomodulatory Functions of Campath-1H (PC01)

description: This is a multicenter, open label, single arm trial to evaluate the safety of alemtuzumab after kidney transplantation as part of a multitherapy regimen to prevent kidney graft loss and death and to avoid steroids and chronic use of calcineurin inhibitors in pediatric renal transplant recipients 1 to 20 years of age.
DOI: 10.21430/m3u2r9iv87
title: Plasmablast response to inactivated and live attenuated influenza vaccines (TIV3/TIV3 ID) in 2012

description: To study the plasmablast response to 2012 seasonal inactivated influenza vaccine
DOI: 10.21430/m3u5abtpcj
title: Antimicrobial Response to Oral Vitamin D3 in Patients with Psoriasis (CATH 03)

description: Atopic Dermatitis (AD) is a skin disorder in which people often have swelling and skin infection. People with this disease cannot receive the smallpox vaccine because it could cause them to have a fatal reaction known as eczema vaccinatum (EV). AD subjects have a lack of antimicrobial peptides (AMPs) under inflammatory conditions. This is a substudy to the ADVN Vitamin D3 study (ADVN CATH 03).
DOI: 10.21430/m3v7caz3np
title: Inner-City Anti-IgE Therapy for Asthma (ICATA/ICAC-08)

description: The purpose of this study is to find out if adding omalizumab to standard asthma treatment results in a safer, more effective, and longer lasting asthma treatment strategy than standard treatment alone, in inner-city children with mild to severe asthma.