DOI: 10.5281/zenodo.1208077
title: Br soaked lysozyme datasets collected with the mixed scheme implemented in HITO in the ZOO system

description: Bromide soaked lysozyme crystals were used as standard crystals for evaluation of the automated data collection, ZOO, developed at SPring-8 BL32XU.

Crystals those sizes ranged 10-100 µm were cryocooled together in the same cryoloop and processed 'mixed scheme' implemented in the ZOO system.

Beam size : 10(H) x 15(V) µm<sup>2</sup>

After 2D raster scanning the loop, HITO detects 1 crystals for full rotation helical, 7 crystals for partial helical, and 62 crystals for multiple small wedge schemes, respectively. A full rotation helical, partial helical and small wedge datasets contain total oscillation of 60 deg, 40 deg and 5 deg. respectively. 

The crystals belong to space group P4<sub>3</sub>2<sub>1</sub>2 with mean unit cell parameters a=b=79.1, c=37.5.

Data was processed up to 1.50 Å resolution in the published result (Hirata et al. in preparation) using KAMO.

<em><strong>&lt;Available data&gt;</strong></em>


	# Beamline:     SPring-8 BL32XU
	# wavelength    0.9000 A
	# Detector :    EIGER X 9M
	# ZOO mode: mixed scheme (HITO)
	#####################################################
	# Multiple small wedge datasets
	# 'CPS1991-05-multi_master.h5' manages 62 datasets
	#####################################################
	# 5.0 deg. / datasets
	# 0.1 deg. / frame
	#  50 frames / datasets
	# 100 frames in each data file
	#####################################################
	hito-CPS1991-05//data/CPS1991-05-multi_data_000001.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000002.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000003.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000004.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000005.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000006.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000007.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000008.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000009.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000010.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000011.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000012.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000013.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000014.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000015.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000016.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000017.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000018.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000019.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000020.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000021.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000022.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000023.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000024.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000025.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000026.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000027.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000028.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000029.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000030.h5
	hito-CPS1991-05//data/CPS1991-05-multi_data_000031.h5
	hito-CPS1991-05//data/CPS1991-05-multi_master.h5


 


	#####################################################
	# 'cry-00-??_master.h5' are for 'partial helical' datasets
	#####################################################
	# 40.0 deg. / datasets
	#  0.1 deg. / frame
	#  400 frames / datasets
	#  100 frames in each data file
	#####################################################
	hito-CPS1991-05//data/cry-00-00_data_000001.h5
	hito-CPS1991-05//data/cry-00-00_data_000002.h5
	hito-CPS1991-05//data/cry-00-00_data_000003.h5
	hito-CPS1991-05//data/cry-00-00_data_000004.h5
	hito-CPS1991-05//data/cry-00-00_master.h5
	hito-CPS1991-05//data/cry-00-01_data_000001.h5
	hito-CPS1991-05//data/cry-00-01_data_000002.h5
	hito-CPS1991-05//data/cry-00-01_data_000003.h5
	hito-CPS1991-05//data/cry-00-01_data_000004.h5
	hito-CPS1991-05//data/cry-00-01_master.h5
	hito-CPS1991-05//data/cry-00-02_data_000001.h5
	hito-CPS1991-05//data/cry-00-02_data_000002.h5
	hito-CPS1991-05//data/cry-00-02_data_000003.h5
	hito-CPS1991-05//data/cry-00-02_data_000004.h5
	hito-CPS1991-05//data/cry-00-02_master.h5
	hito-CPS1991-05//data/cry-00-03_data_000001.h5
	hito-CPS1991-05//data/cry-00-03_data_000002.h5
	hito-CPS1991-05//data/cry-00-03_data_000003.h5
	hito-CPS1991-05//data/cry-00-03_data_000004.h5
	hito-CPS1991-05//data/cry-00-03_master.h5
	hito-CPS1991-05//data/cry-00-04_data_000001.h5
	hito-CPS1991-05//data/cry-00-04_data_000002.h5
	hito-CPS1991-05//data/cry-00-04_data_000003.h5
	hito-CPS1991-05//data/cry-00-04_data_000004.h5
	hito-CPS1991-05//data/cry-00-04_master.h5
	hito-CPS1991-05//data/cry-00-05_data_000001.h5
	hito-CPS1991-05//data/cry-00-05_data_000002.h5
	hito-CPS1991-05//data/cry-00-05_data_000003.h5
	hito-CPS1991-05//data/cry-00-05_data_000004.h5
	hito-CPS1991-05//data/cry-00-05_master.h5
	hito-CPS1991-05//data/cry-00-06_data_000001.h5
	hito-CPS1991-05//data/cry-00-06_data_000002.h5
	hito-CPS1991-05//data/cry-00-06_data_000003.h5
	hito-CPS1991-05//data/cry-00-06_data_000004.h5
	hito-CPS1991-05//data/cry-00-06_master.h5


 


	#####################################################
	# 'cry-01-??_master.h5' are for 'full rotation helical' datasets
	#####################################################
	# 60.0 deg. / datasets
	#  0.1 deg. / frame
	#  600 frames / datasets
	#  100 frames in each data file
	# ** The program could collect 'full rotation' helical dataset
	# but 60 deg. was set for this demonstration.
	#####################################################
	hito-CPS1991-05//data/cry-01-00_data_000001.h5
	hito-CPS1991-05//data/cry-01-00_data_000002.h5
	hito-CPS1991-05//data/cry-01-00_data_000003.h5
	hito-CPS1991-05//data/cry-01-00_data_000004.h5
	hito-CPS1991-05//data/cry-01-00_data_000005.h5
	hito-CPS1991-05//data/cry-01-00_data_000006.h5
	hito-CPS1991-05//data/cry-01-00_master.h5