cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.7yw8em
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ginkgoopsida)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((150 -30,180 -30,180 0,150 0,150 -30))"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052139-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5wrys3
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ginkgoopsida)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((120 -30,150 -30,150 0,120 0,120 -30))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052141-250920141307145/datasets/export for details.
DOI: 10.6084/m9.figshare.29974303
title: The protein composition of 10A EV and 231 EV samples

description: Extracellular vesicles (EVs) were isolated from 231 and 10A cell lines using differential centrifugation. The harvested EVs were lysed with a lysis buffer containing 8 M urea with ultrasonication, followed by centrifugation at 12,000 × g for 30 min at 4 °C. The protein supernatant was collected, and total protein concentration was determined using a BCA assay.For proteomic analysis, 100 μg of protein was subjected to reduction with 10 mM tris and 100 mM triethylammonium bicarbonate (TEAB) at 37 °C for 60 min, followed by alkylation with 40 mM iodoacetamide (IAM) at room temperature for 40 min. Proteins were precipitated using pre-chilled acetone (6:1, v/v) at −20 °C for 4 h, pelleted by centrifugation at 10,000 g for 20 min, and resuspended in 100 µL of 100 mM TEAB. Digestion was performed with trypsin at a 1:50 enzyme-to-protein ratio overnight at 37 °C.The resulting peptides were labeled using TMT10-plex reagents (Thermo Fisher, Cat# 90111) according to the manufacturer’s instructions. Specifically, TMT10-126 and TMT10-130N were used to label peptides from 231_EVs and 10A_EVs, respectively. Equal amounts of labeled peptides from each group were pooled, dried under vacuum, and reconstituted in UPLC loading buffer (2% acetonitrile, pH 10 adjusted with ammonia water).<br>High-pH reverse-phase liquid chromatography (RPLC) was performed using an ACQUITY UPLC BEH C18 Column (1.7 µm, 2.1 mm × 150 mm; Waters) on a Thermo Scientific Vanquish Flex UHPLC system. The separation used a gradient from 0% to 100% buffer B (80% acetonitrile, pH 10) over 48 min at a flow rate of 200 μL/min. A total of 20 fractions were collected based on UV absorption at 214 nm, pooled into 10 fractions, concentrated, and reconstituted in LC-MS loading buffer (2% acetonitrile, 0.1% formic acid).The fractions were analyzed by LC–MS/MS using an EASY-nLC 1200 system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated on a C18 column (75 μm × 25 cm) over a 120-min gradient from 8% to 48% buffer B (80% acetonitrile, 0.1% formic acid) at a flow rate of 300 nL/min. MS data were acquired in data-dependent acquisition (DDA) mode with a full scan range of 350–1500 m/z at 60,000 resolution. MS2 spectra were generated using HCD fragmentation at 30,000 resolution with a dynamic exclusion time of 35 s.Raw files were processed using Proteome Discoverer software (v2.4) and searched against a specified protein database. Peptide identifications were filtered at a false discovery rate (FDR) of ≤ 0.01.<br>
DOI: 10.6084/m9.figshare.29974303.v2
title: The protein composition of 10A EV and 231 EV samples

description: Extracellular vesicles (EVs) were isolated from 231 and 10A cell lines using differential centrifugation. The harvested EVs were lysed with a lysis buffer containing 8 M urea with ultrasonication, followed by centrifugation at 12,000 × g for 30 min at 4 °C. The protein supernatant was collected, and total protein concentration was determined using a BCA assay.For proteomic analysis, 100 μg of protein was subjected to reduction with 10 mM tris and 100 mM triethylammonium bicarbonate (TEAB) at 37 °C for 60 min, followed by alkylation with 40 mM iodoacetamide (IAM) at room temperature for 40 min. Proteins were precipitated using pre-chilled acetone (6:1, v/v) at −20 °C for 4 h, pelleted by centrifugation at 10,000 g for 20 min, and resuspended in 100 µL of 100 mM TEAB. Digestion was performed with trypsin at a 1:50 enzyme-to-protein ratio overnight at 37 °C.The resulting peptides were labeled using TMT10-plex reagents (Thermo Fisher, Cat# 90111) according to the manufacturer’s instructions. Specifically, TMT10-126 and TMT10-130N were used to label peptides from 231_EVs and 10A_EVs, respectively. Equal amounts of labeled peptides from each group were pooled, dried under vacuum, and reconstituted in UPLC loading buffer (2% acetonitrile, pH 10 adjusted with ammonia water).<br>High-pH reverse-phase liquid chromatography (RPLC) was performed using an ACQUITY UPLC BEH C18 Column (1.7 µm, 2.1 mm × 150 mm; Waters) on a Thermo Scientific Vanquish Flex UHPLC system. The separation used a gradient from 0% to 100% buffer B (80% acetonitrile, pH 10) over 48 min at a flow rate of 200 μL/min. A total of 20 fractions were collected based on UV absorption at 214 nm, pooled into 10 fractions, concentrated, and reconstituted in LC-MS loading buffer (2% acetonitrile, 0.1% formic acid).The fractions were analyzed by LC–MS/MS using an EASY-nLC 1200 system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated on a C18 column (75 μm × 25 cm) over a 120-min gradient from 8% to 48% buffer B (80% acetonitrile, 0.1% formic acid) at a flow rate of 300 nL/min. MS data were acquired in data-dependent acquisition (DDA) mode with a full scan range of 350–1500 m/z at 60,000 resolution. MS2 spectra were generated using HCD fragmentation at 30,000 resolution with a dynamic exclusion time of 35 s.Raw files were processed using Proteome Discoverer software (v2.4) and searched against a specified protein database. Peptide identifications were filtered at a false discovery rate (FDR) of ≤ 0.01.<br>
DOI: 10.6084/m9.figshare.29925713
title: Targeted mass spectrometry detection of citrulline

description: For intracellular citrulline measurement, cells (231/Ctrl and 231/DDAH1 KO) were harvested from 15-cm dishes after trypsinization and centrifuged (300 × g, 5 min). Supernatants were discarded, and intact cell pellets were retained for subsequent citrulline analysis. To measure the enzymatic activity of EV-derived DDAH1, extracellular vesicles (EVs) were purified from MCF-10A and MDA-MB-231 cells via differential centrifugation. Purified EVs were lysed in buffer containing 1% Triton X-100, 5 mM 1,10-phenanthroline, and 5 mM tris(2-carboxyethyl)phosphine (TCEP) (pH 8.5). The EV lysate was then incubated with 10 mM ADMA in 50 mM Tris buffer at 37°C for 1 hour prior to mass spectrometry (MS) analysis.Samples were mixed with 1 mL of ice-cold methanol/acetonitrile/H₂O (2:2:1, v/v/v) for metabolite extraction. After homogenization, samples underwent three sonication-freeze-thaw cycles using liquid nitrogen. Proteins were precipitated at -20°C for 1 h, followed by centrifugation at 14,000×g for 15 min (4°C). The supernatant was collected and dried in a vacuum concentrator. For LC-MS analysis, dried extracts were reconstituted in 100 μL of acetonitrile/water (1:1, v/v).The dried extract was resuspended in 50% acetonitrile and analyzed by ultraperformance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) conducted on a Waters Acquity UPLC-system coupled with 5500 QTRAP system (SCIEX). For the analysis of citrulline, Chromatographic separation was achieved on a Waters Acquity UPLC HSS T3 Column (2.1 mm× 100 mm, 1.7 μm, Waters) using a flow rate of 0.3 mL/min at 40°C during a 10 min gradient (0–2 min from 95% B, 2–4 min from 95% B to 50% B, 4–7 min 50% B, 7–7.1 min from 50% B to 95% B, 7.1–10 min 95% B), using buffer A (20 mM ammonium acetate, 20 mM ammonia solution and 5% (v/v) acetonitrile in water) and buffer B (acetonitrile). Mass spectrometry was operated in positive mode using an ESI source. The citrulline was monitored in multiple reaction monitoring (MRM) mode using the optimized precursor-to-product ion transitions. Peak determination and area integration were performed using Analyst 1.7.1 and SCIEX OS 1.4.0 software
DOI: 10.6084/m9.figshare.29925713.v1
title: Targeted mass spectrometry detection of citrulline

description: For intracellular citrulline measurement, cells (231/Ctrl and 231/DDAH1 KO) were harvested from 15-cm dishes after trypsinization and centrifuged (300 × g, 5 min). Supernatants were discarded, and intact cell pellets were retained for subsequent citrulline analysis. To measure the enzymatic activity of EV-derived DDAH1, extracellular vesicles (EVs) were purified from MCF-10A and MDA-MB-231 cells via differential centrifugation. Purified EVs were lysed in buffer containing 1% Triton X-100, 5 mM 1,10-phenanthroline, and 5 mM tris(2-carboxyethyl)phosphine (TCEP) (pH 8.5). The EV lysate was then incubated with 10 mM ADMA in 50 mM Tris buffer at 37°C for 1 hour prior to mass spectrometry (MS) analysis.Samples were mixed with 1 mL of ice-cold methanol/acetonitrile/H₂O (2:2:1, v/v/v) for metabolite extraction. After homogenization, samples underwent three sonication-freeze-thaw cycles using liquid nitrogen. Proteins were precipitated at -20°C for 1 h, followed by centrifugation at 14,000×g for 15 min (4°C). The supernatant was collected and dried in a vacuum concentrator. For LC-MS analysis, dried extracts were reconstituted in 100 μL of acetonitrile/water (1:1, v/v).The dried extract was resuspended in 50% acetonitrile and analyzed by ultraperformance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) conducted on a Waters Acquity UPLC-system coupled with 5500 QTRAP system (SCIEX). For the analysis of citrulline, Chromatographic separation was achieved on a Waters Acquity UPLC HSS T3 Column (2.1 mm× 100 mm, 1.7 μm, Waters) using a flow rate of 0.3 mL/min at 40°C during a 10 min gradient (0–2 min from 95% B, 2–4 min from 95% B to 50% B, 4–7 min 50% B, 7–7.1 min from 50% B to 95% B, 7.1–10 min 95% B), using buffer A (20 mM ammonium acetate, 20 mM ammonia solution and 5% (v/v) acetonitrile in water) and buffer B (acetonitrile). Mass spectrometry was operated in positive mode using an ESI source. The citrulline was monitored in multiple reaction monitoring (MRM) mode using the optimized precursor-to-product ion transitions. Peak determination and area integration were performed using Analyst 1.7.1 and SCIEX OS 1.4.0 software
DOI: 10.6084/m9.figshare.29974303.v1
title: The protein composition of 10A EV and 231 EV samples

description: Extracellular vesicles (EVs) were isolated from 231 and 10A cell lines using differential centrifugation. The harvested EVs were lysed with a lysis buffer containing 8 M urea with ultrasonication, followed by centrifugation at 12,000 × g for 30 min at 4 °C. The protein supernatant was collected, and total protein concentration was determined using a BCA assay.For proteomic analysis, 100 μg of protein was subjected to reduction with 10 mM tris and 100 mM triethylammonium bicarbonate (TEAB) at 37 °C for 60 min, followed by alkylation with 40 mM iodoacetamide (IAM) at room temperature for 40 min. Proteins were precipitated using pre-chilled acetone (6:1, v/v) at −20 °C for 4 h, pelleted by centrifugation at 10,000 g for 20 min, and resuspended in 100 µL of 100 mM TEAB. Digestion was performed with trypsin at a 1:50 enzyme-to-protein ratio overnight at 37 °C.The resulting peptides were labeled using TMT10-plex reagents (Thermo Fisher, Cat# 90111) according to the manufacturer’s instructions. Specifically, TMT10-126 and TMT10-130N were used to label peptides from 231_EVs and 10A_EVs, respectively. Equal amounts of labeled peptides from each group were pooled, dried under vacuum, and reconstituted in UPLC loading buffer (2% acetonitrile, pH 10 adjusted with ammonia water).<br>High-pH reverse-phase liquid chromatography (RPLC) was performed using an ACQUITY UPLC BEH C18 Column (1.7 µm, 2.1 mm × 150 mm; Waters) on a Thermo Scientific Vanquish Flex UHPLC system. The separation used a gradient from 0% to 100% buffer B (80% acetonitrile, pH 10) over 48 min at a flow rate of 200 μL/min. A total of 20 fractions were collected based on UV absorption at 214 nm, pooled into 10 fractions, concentrated, and reconstituted in LC-MS loading buffer (2% acetonitrile, 0.1% formic acid).The fractions were analyzed by LC–MS/MS using an EASY-nLC 1200 system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated on a C18 column (75 μm × 25 cm) over a 120-min gradient from 8% to 48% buffer B (80% acetonitrile, 0.1% formic acid) at a flow rate of 300 nL/min. MS data were acquired in data-dependent acquisition (DDA) mode with a full scan range of 350–1500 m/z at 60,000 resolution. MS2 spectra were generated using HCD fragmentation at 30,000 resolution with a dynamic exclusion time of 35 s.Raw files were processed using Proteome Discoverer software (v2.4) and searched against a specified protein database. Peptide identifications were filtered at a false discovery rate (FDR) of ≤ 0.01.<br>
DOI: 10.15468/dl.vudgf8
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ginkgoopsida)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((90 -30,120 -30,120 0,90 0,90 -30))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052142-250920141307145/datasets/export for details.
DOI: 10.15468/dl.4zpnah
title: Occurrence Download

description: A dataset containing 5469 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is New Zealand",
 "DatasetKey is iNaturalist Research-grade Observations",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Issue is Continent derived from coordinates",
 "TaxonKey is Piper excelsum"
 ]
}

 The dataset includes 5469 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052143-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ydx5tx
title: Occurrence Download

description: A dataset containing 357 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Diospyros lotus",
 "Country is France",
 "HasCoordinate is true"
 ]
}

 The dataset includes 357 records from 37 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052144-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.v9u5cy
title: Occurrence Download

description: A dataset containing 199 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Diospyros lotus",
 "Country is Italy",
 "HasCoordinate is true"
 ]
}

 The dataset includes 199 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052145-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.35njfu
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ginkgoopsida)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((60 -30,90 -30,90 0,60 0,60 -30))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052146-250920141307145/datasets/export for details.
DOI: 10.15468/dl.22dabr
title: Occurrence Download

description: A dataset containing 469 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Camellia euryoides)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 469 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052147-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.26023/5hnd-bxc0-yn14
title: Other: ARM NSA Radiosonde Data Set. Version 1.0

description: This data set contains the radiosonde observations from the Coordinated Energy and Water Cycle Observation Project (CEOP) Climate Prediction Program for the Americas (CPPA) ARM North Slope of Alaska (NSA) reference site in Barrow, Alaska. This data set includes 2-sec vertical resolution observations of temperature, pressure, dew point, wind speed, wind direction, and relative humidity. This data set covers the time period from 1 October 2002 through 31 December 2009. Further information about the ARM NSA site is available at http://www.arm.gov . These data are in monthly zip files (1-5 Mb Each) containing netcdf files. These data have not been processed or quality controlled by NCAR/EOL.
DOI: 10.15468/dl.pnffvk
title: Occurrence Download

description: A dataset containing 15085 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Desmodus rotundus"
 ]
}

 The dataset includes 15085 records from 346 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0052148-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/independent-c69-37250
title: Summary of The progressives and the police, 1991-06-17

description: Memo - re: dissertation summary, "The progressives and the police: urban reform and the professionalization of the Los Angeles police" by Joseph G Woods - from: Diana M Torres; to: Thomas Hungar, 1991 June 17. PART OF A SERIES: O'Melveny & Myers, the firm for which Commission Chairman Warren Christopher worked, conducted research pertaining to the structure and organization of the LAPD. Included in the series are materials related to the firm's research and work product, including excerpts from city charter revisions, summaries of dissertations and books about organizational control of the police, memoranda on various structural issues facing both the LAPD and the Commission's investigation, an excerpt from the Manual of the Los Angeles Police Department, and a transcript of court proceedings in Collins v. City of Los Angeles, a civil case in which a female African-American police recruit filed a lawsuit against the city on the grounds of racial and gender discrimination.
DOI: 10.15468/dl.gf3bku
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Silene conglomeratica Melzh."
 ]
}

 The dataset includes 5 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002705-150221044754056/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.n7amj4
title: Occurrence Download

description: A dataset containing 30 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 }
 ]
 },
 "TaxonKey is Craugastor chrysozetetes (McCranie, Savage & Wilson, 1989)"
 ]
}

 The dataset includes 30 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0037559-240229165702484/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.p0dict
title: Occurrence Download

description: A dataset containing 66 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Tillandsia vicentina Standl."
 ]
}

 The dataset includes 66 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002705-150615163101818/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/independent-c69-30050
title: 6.79. IC on LAPD / general counsel - Godwin, Lt. George, 1991-07-17

description: 6.79. Independent Commission on Los Angeles Police Department / general counsel - Godwin, Lieutanant George, 1991 July 17. Thank you letters from George Godwin. PART OF A SERIES: The series pertains to the work of Munger, Tolles & Olson, the firm responsible for coordinating the activities and proceedings of the Independent Commission's legal counsel. Included are legal briefs, court transcripts, and memoranda; press releases and article clippings about the Rodney King incident and the subsequent creation of the Independent Commission; summaries of witness interviews; analyses of legislation regarding the role and purview of peace officers; work plans and outlines developed by Commission staff; draft copies developed for all sections of the Commission report; supplemental materials for the report, including a copy of the Rodney King arrest log, law enforcement literature, and public opinion surveys; materials distributed and reviewed during Commission meetings; letters of interest submitted to Chairman Christopher from prospective Commission members and organizations; and lists of bills and Commission expenditures.
DOI: 10.15468/dl.pebfsm
title: Occurrence Download

description: A dataset containing 603889 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-162.50329 1.19717,-22.26724 1.19717,-22.26724 57.29159,-162.50329 57.29159,-162.50329 1.19717))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Tyrannus vociferans Swainson, 1826"
 ]
}

 The dataset includes 603889 records from 96 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006289-230828120925497/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.meajro
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Ips mannsfeldi Wood & Bright, 1992"
 ]
}

 The dataset includes 5 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002705-150912100344238/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.