cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.16870664
title: Pathogenic mechanisms of preeclampsia with severe features implied by the plasma exosomal mirna profile

description: Preeclampsia is a complication of pregnancy characterized by high blood pressure and organ damage after 20 gestational weeks. It is associated with high maternal and fetal morbidity and mortality. However, at present, there is no effective prevention or treatment for this condition. Previous studies have revealed that plasma exosomal mirnas from pregnant women with preeclampsia could serve as biomarkers of pathogenic factors. However, the roles of plasma exosomal mirnas in preeclampsia with severe features (sPE), which is associated with poorer pregnancy outcomes, remain unknown. Thus, the aims of this study were to characterize plasma exosomal miRNAs in sPE and explore the related pathogenic mechanisms using bioinformatic analysis. Plasma exosomes were isolated using a mirVana RNA isolation kit. the exosomal miRNAs were detected using high-throughput sequencing and the mirnas related to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene ontology (GO) terms were analyzed using the clusterprofiler package of R. Fifteen miRNAs exhibited increased expression and fourteen miRNAs exhibited reduced expression in plasma exosomes from women with sPE as compared to normal pregnant women. Further, gene set enrichment analysis revealed that the differentially expressed plasma exosomal miRNAs were related to the stress response and cell junction regulation, among others. In summary, this study is the first to identify the differentially expressed plasma exosomal miRNAs in sPE. These findings highlight promising pathogenesis mechanisms underlying preeclampsia.
DOI: 10.5281/zenodo.10635207
title: data for a CiPEHR DA study

description: This dataset is for a data model fusion study from a 8-year permafrost warming experiement at Alaska using a terrestrial ecosysm model (TECO). The dataset includes model inputs (environmental variables), model outputs, observational data for data assimilation, and computer codes of the TECO model.
DOI: 10.5281/zenodo.10635208
title: data for a CiPEHR DA study

description: This dataset is for a data model fusion study from a 8-year permafrost warming experiement at Alaska using a terrestrial ecosysm model (TECO). The dataset includes model inputs (environmental variables), model outputs, observational data for data assimilation, and computer codes of the TECO model.
DOI: 10.6084/m9.figshare.14766210
title: MIR210HG regulates glycolysis, cell proliferation, and metastasis of pancreatic cancer cells through miR-125b-5p/HK2/PKM2 axis

description: Pancreatic cancer has the worst prognosis of all common cancers. Pancreatic cancer cells have a metabolic advantage due to their swiftly adaptive responses to hypoxic and low-nutrient medium. This advantage contributes to the aggressivity of pancreatic cancer. In this study, lncRNA MIR210HG was abnormally upregulated within pancreatic cancer. It acted as a key oncogenic regulator of pancreatic cancer aggressiveness and glycolysis. Knockdown of MIR210HG significantly inhibited the aggressive phenotype of pancreatic cancer cells and inhibited the growth of xenograft tumours. More importantly, MIR210HG knockdown inhibited pancreatic cancer cell glycolysis via regulating the glycolysis-related hexokinase 2 (HK2) and Pyruvate kinase muscle isozyme M2 (PKM2) expression. Compared with the MIR210HG knockdown group, miR-125b-5p inhibition promoted the aggressive phenotypes and glycolysis of pancreatic cancer cells. Furthermore, the effects of MIR210HG knockdown on HK2 and PKM2 expression, pancreatic cancer cell aggressive phenotypes, and glycolysis were significantly reversed by miR-125b-5p inhibition. In tissue samples, MIR210HG expression was negatively correlated with miR-125b-5p levels and positively correlated with HK2 and PKM2 expression. miR-125b-5p expression was negatively correlated with HK2 and PKM2 expression. In conclusion, MIR210HG affected the phenotypes of pancreatic cancer cells, including proliferation, invasion, migration, and glycolysis, via modulating the miR-125b-5p/HK2/PKM2 axis.
DOI: 10.6084/m9.figshare.14766213
title: New substrates and determinants for tRNA recognition of RNA methyltransferase DNMT2/TRDMT1

description: Methylation is a common post-transcriptional modification of tRNAs, particularly in the anticodon loop region. The cytosine 38 (C38) in tRNAs, such as tRNA<sup>Asp-GUC</sup>, tRNA<sup>Gly-GCC</sup>, tRNA<sup>Val-AAC</sup>, and tRNA<sup>Glu-CUC</sup>, can be methylated by human DNMT2/TRDMT1 and some homologs found in bacteria, plants, and animals. However, the substrate properties and recognition mechanism of DNMT2/TRDMT1 remain to be explored. Here, taking into consideration common features of the four known substrate tRNAs, we investigated methylation activities of DNMT2/TRDMT1 on the tRNA<sup>Gly-GCC</sup> truncation and point mutants, and conformational changes of mutants. The results demonstrated that human DNMT2/TRDMT1 preferred substrate tRNA<sup>Gly-GCC</sup> in vitro. L-shaped conformation of classical tRNA could be favourable for DNMT2/TRDMT1 activity. The complete sequence and structure of tRNA were dispensable for DNMT2/TRDMT1 activity, whereas T-arm was indispensable to this activity. G19, U20, and A21 in D-loop were identified as the important bases for DNMT2/TRDMT1 activity, while G53, C56, A58, and C61 in T-loop were found as the critical bases. The conserved CUXXCAC sequence in the anticodon loop was confirmed to be the most critical determinant, and it could stabilize C38-flipping to promote C38 methylation. Based on these tRNA properties, new substrates, tRNA<sup>Val-CAC</sup> and tRNA<sup>Gln-CUG</sup>, were discovered in vitro. Moreover, a single nucleotide substitute, U32C, could convert non-substrate tRNA<sup>Ala-AGC</sup> into a substrate for DNMT2/TRDMT1. Altogether, our findings imply that DNMT2/TRDMT1 relies on a delicate network involving both the primary sequence and tertiary structure of tRNA for substrate recognition.
DOI: 10.6084/m9.figshare.7950395.v1
title: MOESM1 of Somatic mutation of DNAH genes implicated higher chemotherapy response rate in gastric adenocarcinoma patients

description: Additional file 1: Table S1. The filtering conditions to detect somatic SNV. Table S2. DNAH mutations in our cohort with 64 FFPE samples of gastric adenocarcinoma.
DOI: 10.6084/m9.figshare.14766213.v1
title: New substrates and determinants for tRNA recognition of RNA methyltransferase DNMT2/TRDMT1

description: Methylation is a common post-transcriptional modification of tRNAs, particularly in the anticodon loop region. The cytosine 38 (C38) in tRNAs, such as tRNA<sup>Asp-GUC</sup>, tRNA<sup>Gly-GCC</sup>, tRNA<sup>Val-AAC</sup>, and tRNA<sup>Glu-CUC</sup>, can be methylated by human DNMT2/TRDMT1 and some homologs found in bacteria, plants, and animals. However, the substrate properties and recognition mechanism of DNMT2/TRDMT1 remain to be explored. Here, taking into consideration common features of the four known substrate tRNAs, we investigated methylation activities of DNMT2/TRDMT1 on the tRNA<sup>Gly-GCC</sup> truncation and point mutants, and conformational changes of mutants. The results demonstrated that human DNMT2/TRDMT1 preferred substrate tRNA<sup>Gly-GCC</sup> in vitro. L-shaped conformation of classical tRNA could be favourable for DNMT2/TRDMT1 activity. The complete sequence and structure of tRNA were dispensable for DNMT2/TRDMT1 activity, whereas T-arm was indispensable to this activity. G19, U20, and A21 in D-loop were identified as the important bases for DNMT2/TRDMT1 activity, while G53, C56, A58, and C61 in T-loop were found as the critical bases. The conserved CUXXCAC sequence in the anticodon loop was confirmed to be the most critical determinant, and it could stabilize C38-flipping to promote C38 methylation. Based on these tRNA properties, new substrates, tRNA<sup>Val-CAC</sup> and tRNA<sup>Gln-CUG</sup>, were discovered in vitro. Moreover, a single nucleotide substitute, U32C, could convert non-substrate tRNA<sup>Ala-AGC</sup> into a substrate for DNMT2/TRDMT1. Altogether, our findings imply that DNMT2/TRDMT1 relies on a delicate network involving both the primary sequence and tertiary structure of tRNA for substrate recognition.
DOI: 10.6084/m9.figshare.21582062.v1
title: Additional file 2 of Identification of risk variants related to malignant tumors in children with birth defects by whole genome sequencing

description: Additional file 2.
DOI: 10.6084/m9.figshare.13193433
title: Detection of an α-Globin Fusion Gene Using Real-Time Polymerase Chain Reaction-Based Multicolor Melting Curve

description: Genetic recombination between homologous sequences on the human globin gene clusters can lead to the creation of fusion genes. In this study, we report the detection of an α-globin fusion gene by using real-time polymerase chain reaction (qPCR)-based multicolor melting curve analysis (MMCA). The carriers of this fusion gene had a mild α-thalassemia phenotype with a normal hemoglobin (Hb) value and borderline hematological indices. Sequence analysis revealed that the mutant gene was the result of a fusion between the α2 and ψα1 genes. Our results indicate that the MMCA has the ability to detect the fusion gene, which is helpful for genetic counseling in thalassemia prevalent areas.
DOI: 10.6084/m9.figshare.24735003
title: ERCP biopsy

description: original data of the research
DOI: 10.6084/m9.figshare.20009215.v1
title: Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels

description: The effect of dietary carbohydrate (CHO) level on serum physiological response, hepatic antioxidant abilities and heat shock protein 70 (HSP70) expression of Wuchang bream (Megalobrama amblycephala) was studied. Two isonitrogenous (28.56% crude protein) and isolipidic (5.28% crude lipid) diets were formulated to contain 30% or 53% wheat starch. Diets were fed for 90 days to fish in triplicate tanks (28 fish per tank). At the end of feeding trial, significantly higher serum triglyceride level, insulin level, cortisol level, and malondialdehyde (MDA) content were observed in fish fed the 53% CHO diet, while significantly lower serum total protein content, alkaline phosphatase activity, superoxide dismutase activity and total antioxidative capacity were found in fish fed the 53% CHO diet compared with those fed the 30% diet. The relative level of hepatic heat shock protein 70 mRNA was significantly higher in the 53% CHO group than that in the 30% CHO at 6, 12 and 48 h after feeding. Ingestion of 53% dietary CHO impacts the nonspecific immune ability and causes metabolic stress in Megalobrama amblycephala.
DOI: 10.6084/m9.figshare.7950752.v1
title: Additional file 2: of Comparative transcriptome analysis and ChIP-sequencing reveals stage-specific gene expression and regulation profiles associated with pollen wall formation in Brassica rapa

description: Table S1. Summary of Brassica rapa assembled transcriptome. Table S2. Statistics of annotation results for Brassica rapa Unigenes. Table S3. The expression of DEGs in floral buds of the fertile and sterile lines (RPKM value). Table S4. The significantly enriched Gene Ontology (GO) terms by DEGs at each stage. Table S5. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway by DEGs at each stage. Table S6. Expressional changes of putative transcription factors. Table S7. GO analysis of DEGs enriched for H3K4me3 or H3K27me3. Table S8. Primers used in this article. (XLSX 971 kb)
DOI: 10.6084/m9.figshare.21582065.v1
title: Additional file 3 of Identification of risk variants related to malignant tumors in children with birth defects by whole genome sequencing

description: Additional file 3.
DOI: 10.6084/m9.figshare.21582059
title: Additional file 1 of Identification of risk variants related to malignant tumors in children with birth defects by whole genome sequencing

description: Additional file 1.
DOI: 10.6084/m9.figshare.20009215
title: Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels

description: The effect of dietary carbohydrate (CHO) level on serum physiological response, hepatic antioxidant abilities and heat shock protein 70 (HSP70) expression of Wuchang bream (Megalobrama amblycephala) was studied. Two isonitrogenous (28.56% crude protein) and isolipidic (5.28% crude lipid) diets were formulated to contain 30% or 53% wheat starch. Diets were fed for 90 days to fish in triplicate tanks (28 fish per tank). At the end of feeding trial, significantly higher serum triglyceride level, insulin level, cortisol level, and malondialdehyde (MDA) content were observed in fish fed the 53% CHO diet, while significantly lower serum total protein content, alkaline phosphatase activity, superoxide dismutase activity and total antioxidative capacity were found in fish fed the 53% CHO diet compared with those fed the 30% diet. The relative level of hepatic heat shock protein 70 mRNA was significantly higher in the 53% CHO group than that in the 30% CHO at 6, 12 and 48 h after feeding. Ingestion of 53% dietary CHO impacts the nonspecific immune ability and causes metabolic stress in Megalobrama amblycephala.
DOI: 10.6084/m9.figshare.21582062
title: Additional file 2 of Identification of risk variants related to malignant tumors in children with birth defects by whole genome sequencing

description: Additional file 2.
DOI: 10.6084/m9.figshare.21582065
title: Additional file 3 of Identification of risk variants related to malignant tumors in children with birth defects by whole genome sequencing

description: Additional file 3.
DOI: 10.2908/isoc_bde15cbc
title: E-banking and e-commerce
title: Banque en ligne et commerce électronique
title: E-Commerce
DOI: 10.2908/isoc_bde15een2
title: Enterprises using the internet for interacting with public authorities by NACE Rev.2 activity
title: Entreprises utilisant l'internet dans les relations avec l'administration publique, par activité de la NACE Rév. 2
title: Unternehmen, die das Internet zur Kommunikation mit öffentlichen Stellen nutzen, nach Aktivitäten der NACE Rev.2
DOI: 10.6084/m9.figshare.13193433.v1
title: Detection of an α-Globin Fusion Gene Using Real-Time Polymerase Chain Reaction-Based Multicolor Melting Curve

description: Genetic recombination between homologous sequences on the human globin gene clusters can lead to the creation of fusion genes. In this study, we report the detection of an α-globin fusion gene by using real-time polymerase chain reaction (qPCR)-based multicolor melting curve analysis (MMCA). The carriers of this fusion gene had a mild α-thalassemia phenotype with a normal hemoglobin (Hb) value and borderline hematological indices. Sequence analysis revealed that the mutant gene was the result of a fusion between the α2 and ψα1 genes. Our results indicate that the MMCA has the ability to detect the fusion gene, which is helpful for genetic counseling in thalassemia prevalent areas.
DOI: 10.2908/isoc_bde15cua
title: Internet use and activities
title: Utilisation de l'internet et activités sur l'internet
title: Internet Nutzung und Tätigkeiten
DOI: 10.2908/isoc_bde15decn2
title: E-commerce, customer relation management (CRM) and secure transactions by NACE Rev.2 activity
title: Commerce électronique, logiciels de gestion de la relation clientèle et transactions sécurisées, par activité de la NACE Rév. 2
title: E-Commerce, CRM-Softwarelösungen und sichere Datenübertragung, nach Aktivitäten der NACE Rev.2
DOI: 10.2908/isoc_bdek_smi
title: Digital single market - promoting e-commerce for individuals
title: Marché unique numérique - promouvoir le commerce électronique pour les particuliers
title: Digitaler Binnenmarkt - Förderung des elektronischen Handels für Einzelpersonen
DOI: 10.2908/isoc_cicci_pb
title: Problems experienced when using cloud services
title: Problèmes rencontrés en utilisant des services de cloud
title: Probleme bei der Nutzung von Cloud Diensten
DOI: 10.2908/isoc_sk_rnct_i
title: Reasons for not having taken a computer course
title: Raisons pour lesquelles un cours sur l'utilisation d'un ordinateur n'a pas été suivi
title: Gründe warum kein Computerkurs besucht wurde
DOI: 10.2908/isoc_cimobi_purp
title: Purpose of mobile internet use
title: But de l'utilisation de l'internet mobile
title: Zweck der mobilen Internetnutzung
DOI: 10.6084/m9.figshare.20009380
title: Study on the polymorphism of POU1F1 gene in sheep

description: ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep), using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP) loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T), C71G (C/G), and C330G (C/G). C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus were detected in Yuxi fat-tailed sheep; their frequencies were 0.75 and 0.25, respectively. C and G allele of C71G locus were only detected in Yuxi fat-tailed and large-tailed Han sheep; their frequencies were 0.87/0.13 and 0.87/0.13, respectively. The cluster analysis based on POU1F1 gene sequence showed that bactrian camel, dromedary, and wild camel clustered first, and dolphin and killer whales clustered according to taxonomy. Although the four species Tibetan antelope, buffalo, goat, and sheep were alone, they got close and the relative genetic relationship was intimate according to the dendrogram. The mutation site analysis of the POU1F1 gene in five sheep populations in this study would be favorable for uncovering the function of POU1F1 gene deeply.
DOI: 10.2908/isoc_bde15ee
title: Unternehmen, die das Internet zur Kommunikation mit öffentlichen Stellen nutzen, nach Unternehmensgrößenklassen
title: Entreprises utilisant l'internet dans les relations avec l'administration publique, par classe de taille d’entreprise
title: Enterprises using the internet for interacting with public authorities by size class of enterprise
DOI: 10.2908/isoc_sk_rtc_i
title: Most recent training course on computer use
title: Dernière formation en informatique
title: Letzter Ausbildungskurs zum Gebrauch von Computern
DOI: 10.2908/isoc_bde15b_p
title: Accès à large bande et connectivité - personnes occupées, par activité de la NACE Rév. 2
title: Breitband und Konnektivität - Beschäftigte, nach Aktivitäten der NACE Rev.2
title: Broadband and connectivity - persons employed by NACE Rev.2 activity
DOI: 10.2908/isoc_cimobi_prb
title: Problems encountered when using mobile internet
title: Problèmes rencontrés lors de l'utilisation de l'internet mobile
title: Probleme bei der mobilen Internetnutzung
DOI: 10.2908/isoc_ci_cfp_pu
title: Individuals - places of computer use
title: Particuliers - lieux d'utilisation des ordinateurs
title: Einzelpersonen - Orte des Gebrauchs von Computern
DOI: 10.2908/isoc_cicci_awobs
title: Awareness about cloud services and reasons for non-use
title: Information sur les services de cloud et motifs de leur non-utilisation
title: Kenntnis von Cloud Diensten und Gründe der Nichtnutzung
DOI: 10.6084/m9.figshare.21582350
title: Additional file 2 of Identification of LsPIN1 gene and its potential functions in rhizome turning of Leymus secalinus

description: Additional file 2: Table S1. The primers used in this study.
DOI: 10.6084/m9.figshare.23686476
title: The CN-OBEE dataset, occupant behavior, thermal environment, and appliance electricity use of a single-family apartment in China

description: Household is the basic unit of a residential community or building. High-resolution, long-term open data are necessary to help study residential electricity consumption, smart home technologies, and electricity flexibility technologies at a household level. 

The dataset provided, namely CN-OBEE, includes the per-minute occupant behavior, thermal environment, and appliance electricity use data from a single-family apartment in Beijing, China, for an entire year (from May 31, 2021, to May 31, 2022) and the hourly meteorological data collected by the nearest national weather station for the same period. The household data are collected by an IoT-based data collection platform (IDCP) composed of gateways, sensors, and cloud servers, which can simultaneously collect the occupant presence, indoor environment, window-opening state, and appliance electricity consumption data.

The dataset is the first detailed occupant behavior and electricity use dataset of Chinese homes publicly available. As one of the regional features, it includes window-opening behavior and the usage of split air conditioners. It can support electricity load shape analysis, electric power prediction, occupant behavior modeling and validation, building energy modeling and validation, thermal comfort analysis, suitability of energy-related measures, and other types of research for residential buildings at a household level.
DOI: 10.6084/m9.figshare.7950881
title: Additional file 4: of De novo transcriptome and phytochemical analyses reveal differentially expressed genes and characteristic secondary metabolites in the original oolong tea (Camellia sinensis) cultivar ‘Tieguanyin’ compared with cultivar ‘Benshan’

description: Table S3. Read count data in transcriptome dataset. (XLSX 4399 kb)
DOI: 10.25549/usctheses-ouc1504260
title: Microsoft Word - Jessica Porter Thesis final
DOI: 10.25549/usctheses-ouc11358744
title: 00001.tif
DOI: 10.25549/usctheses-ouc11358745
title: 00001.tif
DOI: 10.18112/openneuro.ds004460.v1.1.0
title: EEG and motion capture data set for a full-body/joystick rotation task
DOI: 10.25549/usctheses-ouc1343647
title: Thesis_Draft 2
DOI: 10.6084/m9.figshare.24210897
title: h=0.3.csv

description: Spectral and sensitivity processing data for a cantilever thickness of 0.3mm.
DOI: 10.6084/m9.figshare.24210897.v1
title: h=0.3.csv

description: Spectral and sensitivity processing data for a cantilever thickness of 0.3mm.
DOI: 10.6084/m9.figshare.20009380.v1
title: Study on the polymorphism of POU1F1 gene in sheep

description: ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep), using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP) loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T), C71G (C/G), and C330G (C/G). C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus were detected in Yuxi fat-tailed sheep; their frequencies were 0.75 and 0.25, respectively. C and G allele of C71G locus were only detected in Yuxi fat-tailed and large-tailed Han sheep; their frequencies were 0.87/0.13 and 0.87/0.13, respectively. The cluster analysis based on POU1F1 gene sequence showed that bactrian camel, dromedary, and wild camel clustered first, and dolphin and killer whales clustered according to taxonomy. Although the four species Tibetan antelope, buffalo, goat, and sheep were alone, they got close and the relative genetic relationship was intimate according to the dendrogram. The mutation site analysis of the POU1F1 gene in five sheep populations in this study would be favorable for uncovering the function of POU1F1 gene deeply.