DOI: 10.5061/dryad.4bq08
title: Data from: Which specimens from a museum collection will yield DNA barcodes? A time series study of spiders in alcohol

description: We report initial results from an ongoing effort to build a library of DNA
 barcode sequences for Dutch spiders and investigate the utility of museum
 collections as a source of specimens for barcoding spiders. Source
 material for the library comes from a combination of specimens freshly
 collected in the field specifically for this project and museum specimens
 collected in the past. For the museum specimens, we focus on 31 species
 that have been frequently collected over the past several decades. A
 series of progressively older specimens representing these 31 species were
 selected for DNA barcoding. Based on the pattern of sequencing successes
 and failures, we find that smaller-bodied species expire before
 larger-bodied species as tissue sources for single-PCR standard DNA
 barcoding. Body size and age of oldest successful DNA barcode are
 significantly correlated after factoring out phylogenetic effects using
 independent contrasts analysis. We found some evidence that extracted DNA
 concentration is correlated with body size and inversely correlated with
 time since collection, but these relationships are neither strong nor
 consistent. DNA was extracted from all specimens using standard
 destructive techniques involving the removal and grinding of tissue. A
 subset of specimens was selected to evaluate nondestructive extraction.
 Nondestructive extractions significantly extended the DNA barcoding shelf
 life of museum specimens, especially small-bodied species, and yielded
 higher DNA concentrations compared to destructive extractions. All primary
 data are publically available through a Dryad archive and the Barcode of
 Life database.
description: Data from: Which
 specimens from a museum collection will yield DNA barcodes? A time series
 study of spiders in alcohol1.
 Milleretal2013DutchSpiderBarcodeDwC.txt. Occurrence data for all specimens
 in the study presented as a tab delimited text file with Darwin Core
 fields, plus custom fields for recording destructive and nondestructive
 sequencing success, DNA sequences, DNA concentration data, and hyperlinks
 to records on BOLD (http://www.boldsystems.org/). 2.
 Milleretal2013DutchSpiderBarcode.kml. Occurrence data in KML (keyhole
 markup language) format that can be opened using Google Earth
 (http://earth.google.com/) to display an interactive map plotting Dutch
 spider specimens included in this study. Data can be filtered by taxon
 using the Places directory. 3. Milleretal2013DutchSpiderBarcode.fasta. All
 sequence data for this study in fasta format. 4.
 Milleretal2013DutchSpiderBarcodeDestructiveExtractions.nex. Nexus files
 generated using Mesquite (http://mesquiteproject.org) for the independent
 contrasts analyses testing the relationship between body size and oldest
 success after accounting for phylogeny. Oldest success calculated based on
 destructive extractions only. 5.
 Milleretal2013DutchSpiderBarcodeAllExtractions.nex. Nexus files generated
 using Mesquite (http://mesquiteproject.org) for the independent contrasts
 analyses testing the relationship between body size and oldest success
 after accounting for phylogeny. Oldest success calculated based on all
 extractions (regardless of destructive vs. nondestructive method). 6.
 Milleretal2013DutchSpiderBarcodeElectronicSupplementaryDocuments.doc.
 Figure S1. Correlations based on independent contrasts.Milleretal2013DutchSpiderBarcodeDryad.zip