cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5885653.v1
title: Laboratory diagnosis of chronic kidney disease in adults: an overview of hospitals inserted in the Portuguese National Health System

description: ABSTRACT Introduction: The laboratory diagnosis of chronic kidney disease (CKD) is a simple and cost-effective procedure that allows the detection of early stages of the disease, which is essential to avoid kidney damage and a life threateaning event. It consists of measuring serum creatinine concentration, urinary albumin concentration and calculating the estimated glomerular filtration rate (eGFR). In 2012, the guidelines for laboratory evaluation of the CKD were published by the Kidney Disease: Improving Global Outcomes (KDIGO). Objectives: This study aimed to evaluate whether the laboratories in hospitals of the Portuguese National Health System follow these guidelines and provide a correct diagnosis of CKD. Material and method: A questionnaire composed of 32 questions was sent to the Clinical Pathology Services of all hospitals inserted in the System. Results: All 49 labs responded that measure serum creatinine, 18 reported measurering eGFR. Ten reported measuring eGFR only if specifically ordered. Forty-four measure total protein and albumin in the urine, three only protein, one albumin alone, and one measure none of them. The type of samples, methods, reagents, equipment, expression units of results and reference intervals varied. Conclusion: There is great variability among laboratories in relation to the methodology of measuring serum creatinine, albumin and total protein in the urine. There are wide variations in the release of results. Most laboratories do not follow the guidelines recommended by the KDIGO 2012. This work indicates that there is a need to develop education and alignment processes in the laboratory diagnosis of CKD in the laboratories installed in hospitals inserted in the Portuguese National Health System.
DOI: 10.6084/m9.figshare.5885650
title: Nasopalatine duct cyst - diagnosis, treatment and postoperative complications: report of two cases

description: ABSTRACT The objectives of this study were to report two cases of nasopalatine duct cyst and to review the relevant literature. In the first case, the lesion was approximately 1.5 cm in size and in the second case 3 cm, both characterized by localized swelling in the hard palate. The two cases presented a radiolucent unilocular image in the region of the incisive foramen. The diagnosis was nasopalatine duct cyst, established by excisional biopsy and anatomopathological examination. The second patient presented a late postoperative infection, four weeks after surgery, and was submitted to antibiotic therapy and drainage. Both patients were followed for more than 30 months, with no signs of recurrence.
DOI: 10.6084/m9.figshare.5885650.v1
title: Nasopalatine duct cyst - diagnosis, treatment and postoperative complications: report of two cases

description: ABSTRACT The objectives of this study were to report two cases of nasopalatine duct cyst and to review the relevant literature. In the first case, the lesion was approximately 1.5 cm in size and in the second case 3 cm, both characterized by localized swelling in the hard palate. The two cases presented a radiolucent unilocular image in the region of the incisive foramen. The diagnosis was nasopalatine duct cyst, established by excisional biopsy and anatomopathological examination. The second patient presented a late postoperative infection, four weeks after surgery, and was submitted to antibiotic therapy and drainage. Both patients were followed for more than 30 months, with no signs of recurrence.
DOI: 10.6084/m9.figshare.5885647
title: Perceptions of health managers and facilitators of the Integrated management of childhood illness (IMCI) in Northeastern Brazil and Peru

description: The objective of this study was to explore the Integrated Management of Childhood Illness (IMCI) strategy through the perceptions of the health managers and facilitators of strategy. A qualitative study was conducted using semi-structured interviews. Fourteen interviews were carried out, eight in the State of Ceará-Brazil and six in Peru, between May and June 2011. In general, participants expressed a positive perception of the IMCI strategy. Also was observed the predominance of the clinical component, compared with the community or the health services component. In Peru unlike Brazil, the government promoted the adoption of the strategy. In conclusion, this study shows a complex intervention of the IMCI strategy, with a predominance of the clinical component, evidencing the limitations in the integration of the components of the strategy.
DOI: 10.6084/m9.figshare.5885647.v1
title: Perceptions of health managers and facilitators of the Integrated management of childhood illness (IMCI) in Northeastern Brazil and Peru

description: The objective of this study was to explore the Integrated Management of Childhood Illness (IMCI) strategy through the perceptions of the health managers and facilitators of strategy. A qualitative study was conducted using semi-structured interviews. Fourteen interviews were carried out, eight in the State of Ceará-Brazil and six in Peru, between May and June 2011. In general, participants expressed a positive perception of the IMCI strategy. Also was observed the predominance of the clinical component, compared with the community or the health services component. In Peru unlike Brazil, the government promoted the adoption of the strategy. In conclusion, this study shows a complex intervention of the IMCI strategy, with a predominance of the clinical component, evidencing the limitations in the integration of the components of the strategy.
DOI: 10.6084/m9.figshare.5885644
title: Pseudothrombocytopenia: incidence and strategy for resolution in clinical laboratory

description: ABSTRACT Introduction: Pseudothrombocytopenia is an important source of pre-analytical problems in clinical laboratory; therefore it must be correctly evaluated. Low spurious platelet counts can lead to misdiagnosis. Objective: This study aims to study the resolution of pseudothrombocytopenia in the laboratory routine of clinical laboratory by standardizing the use of vortexer. Methods: This is a cross-sectional, analytical, quantitative study based on experimental approach, involving 45 pseudothrombocytopenia samples divided into three groups and submitted to vortexing for 1, 2 and 3 minutes. Results: During the study, 28,435 blood counts were performed, and 56 (0.196%) of these samples confirmed platelet aggregates. The 2 and 3 minute times normalized the platelet count (greater than 150,000/µl) in two thirds of the samples tested. Platelets, hemolysis index, platelet distribution width (PDW) and mean platelet volume (MPV) presented statistically significant mean difference (MD) (p &lt; 0.05) after vortexing at all times. Conclusion: The 2 and 3-minute times were the most effective in the resolution of pseudothrombocytopenia, however, the 2 minutes time should be preferred because it produced fewer effects on the erythrocyte membrane.
DOI: 10.6084/m9.figshare.5885644.v1
title: Pseudothrombocytopenia: incidence and strategy for resolution in clinical laboratory

description: ABSTRACT Introduction: Pseudothrombocytopenia is an important source of pre-analytical problems in clinical laboratory; therefore it must be correctly evaluated. Low spurious platelet counts can lead to misdiagnosis. Objective: This study aims to study the resolution of pseudothrombocytopenia in the laboratory routine of clinical laboratory by standardizing the use of vortexer. Methods: This is a cross-sectional, analytical, quantitative study based on experimental approach, involving 45 pseudothrombocytopenia samples divided into three groups and submitted to vortexing for 1, 2 and 3 minutes. Results: During the study, 28,435 blood counts were performed, and 56 (0.196%) of these samples confirmed platelet aggregates. The 2 and 3 minute times normalized the platelet count (greater than 150,000/µl) in two thirds of the samples tested. Platelets, hemolysis index, platelet distribution width (PDW) and mean platelet volume (MPV) presented statistically significant mean difference (MD) (p &lt; 0.05) after vortexing at all times. Conclusion: The 2 and 3-minute times were the most effective in the resolution of pseudothrombocytopenia, however, the 2 minutes time should be preferred because it produced fewer effects on the erythrocyte membrane.
DOI: 10.6084/m9.figshare.5885638
title: Different levels of shade on population of grasshoppers and its oviposition preference on heliconias

description: ABSTRACT: Grasshoppers of the families Acrididae and Romaleidae (Orthoptera) are among the insects that defoliate heliconias and have been gaining status as pests of commercial crops of these plants in Brazil. The objectives of the present study were to identify the grasshopper defoliating heliconias in the municipality of Santo Antônio de Pádua, RJ (Brazil), to evaluate the effect of different levels of shade on the population of this grasshopper and the production parameters of heliconias, and to determine if this grasshopper has an oviposition preference among the heliconias evaluated. The experiment was in a completely randomized block design, in subdivided plots (four levels of shade in the plot, 0%, 30%, 50% and 80%, and four species of Heliconia: H. psittacorum, H. stricta, H. wagneriana and H. psittacorum x H. spathocircinata ‘Golden Torch’ in the subplot), with four replications. The grasshopper was identified as Cornops frenatum frenatum (Acrididae). An increase in shade resulted in a decrease in the number of oviposition holes from the grasshopper and the number of lateral buds. Shade did not influence the number of C. f. frenatum nymphs and adults and the number of flower stems. H. wagneriana was the most preferred species for oviposition by C. f. frenatum. Results suggested using screens to shade heliconia plants can help control C. f. frenatum populations, however, the light requirements of the heliconias should be considered to guarantee productivity.
DOI: 10.6084/m9.figshare.5885638.v1
title: Different levels of shade on population of grasshoppers and its oviposition preference on heliconias

description: ABSTRACT: Grasshoppers of the families Acrididae and Romaleidae (Orthoptera) are among the insects that defoliate heliconias and have been gaining status as pests of commercial crops of these plants in Brazil. The objectives of the present study were to identify the grasshopper defoliating heliconias in the municipality of Santo Antônio de Pádua, RJ (Brazil), to evaluate the effect of different levels of shade on the population of this grasshopper and the production parameters of heliconias, and to determine if this grasshopper has an oviposition preference among the heliconias evaluated. The experiment was in a completely randomized block design, in subdivided plots (four levels of shade in the plot, 0%, 30%, 50% and 80%, and four species of Heliconia: H. psittacorum, H. stricta, H. wagneriana and H. psittacorum x H. spathocircinata ‘Golden Torch’ in the subplot), with four replications. The grasshopper was identified as Cornops frenatum frenatum (Acrididae). An increase in shade resulted in a decrease in the number of oviposition holes from the grasshopper and the number of lateral buds. Shade did not influence the number of C. f. frenatum nymphs and adults and the number of flower stems. H. wagneriana was the most preferred species for oviposition by C. f. frenatum. Results suggested using screens to shade heliconia plants can help control C. f. frenatum populations, however, the light requirements of the heliconias should be considered to guarantee productivity.
DOI: 10.6084/m9.figshare.5885629
title: Quorum sensing in Enterococcus faecium, Enterococcus faecalis and Bacillus cereus strains isolated from ricotta processing

description: ABSTRACT: The quorum sensing phenomenon is a process of intra- and inter-species microbial communication involving the production and detection of extracellular signaling molecules. The autoinducer AI-2 has been proposed to serve as a ‘universal signal’ for interspecies communication. This study aimed to evaluate the capability of Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus strains isolated from ricotta processing to produce quorum sensing signalling molecules (AI-2). The strains were evaluated for the presence of the luxS gene using the polymerase chain reaction. AI-2 quorum sensing signalling molecules were measured in relative light units (RLUs) using a luminometer. A total of 74% of E. faecium, 91% of E. faecalis, and 95% of B. cereus isolates were positive for luxS gene. In addition, the induced bioluminescence in Vibrio harveyi BB170 was observed in all strains, indicating the presence of the AI-2 autoinducer.
DOI: 10.6084/m9.figshare.5885629.v1
title: Quorum sensing in Enterococcus faecium, Enterococcus faecalis and Bacillus cereus strains isolated from ricotta processing

description: ABSTRACT: The quorum sensing phenomenon is a process of intra- and inter-species microbial communication involving the production and detection of extracellular signaling molecules. The autoinducer AI-2 has been proposed to serve as a ‘universal signal’ for interspecies communication. This study aimed to evaluate the capability of Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus strains isolated from ricotta processing to produce quorum sensing signalling molecules (AI-2). The strains were evaluated for the presence of the luxS gene using the polymerase chain reaction. AI-2 quorum sensing signalling molecules were measured in relative light units (RLUs) using a luminometer. A total of 74% of E. faecium, 91% of E. faecalis, and 95% of B. cereus isolates were positive for luxS gene. In addition, the induced bioluminescence in Vibrio harveyi BB170 was observed in all strains, indicating the presence of the AI-2 autoinducer.
DOI: 10.6084/m9.figshare.5885626
title: Conservative therapy for central giant cell lesion: case report

description: ABSTRACT Case report of central giant cell lesion (CGCL) in a 32-year-old female patient, who exhibited asymptomatic increase in mandible volume and integrity of the adjacent alveolar mucosa, she was submitted to conservative treatment. The imaging exams showed radiolucent and multilocular lesion with displacement and discrete root resorption of the teeth 34 and 35. An incisional biopsy was performed and the final diagnosis of CGCL was established. The patient was submitted to biweekly applications of triamcinolone hexacetonide, showing no recurrence and exhibiting bone reintegration. Treatment of extensive lesions with intralesional corticosteroid injections has shown satisfactory effects.
DOI: 10.6084/m9.figshare.5885626.v1
title: Conservative therapy for central giant cell lesion: case report

description: ABSTRACT Case report of central giant cell lesion (CGCL) in a 32-year-old female patient, who exhibited asymptomatic increase in mandible volume and integrity of the adjacent alveolar mucosa, she was submitted to conservative treatment. The imaging exams showed radiolucent and multilocular lesion with displacement and discrete root resorption of the teeth 34 and 35. An incisional biopsy was performed and the final diagnosis of CGCL was established. The patient was submitted to biweekly applications of triamcinolone hexacetonide, showing no recurrence and exhibiting bone reintegration. Treatment of extensive lesions with intralesional corticosteroid injections has shown satisfactory effects.
DOI: 10.6084/m9.figshare.5885614
title: The section on Creation at the journal Interface: twenty years of experimentation

description: To celebrate the 20th anniversary of the journal Interface, members of the team responsible for the Creation section immersed themselves in a range of materials that have been published in the section since the journal’s very first issue. The result is an assortment of images, words and reflections that gives an insight as to the multiplicity of voices that tell this story. They represent attempts to think about the Creation section both nowadays and in the past, along with possible pathways for its on-going – and essential – reinvention.
DOI: 10.6084/m9.figshare.5885614.v1
title: The section on Creation at the journal Interface: twenty years of experimentation

description: To celebrate the 20th anniversary of the journal Interface, members of the team responsible for the Creation section immersed themselves in a range of materials that have been published in the section since the journal’s very first issue. The result is an assortment of images, words and reflections that gives an insight as to the multiplicity of voices that tell this story. They represent attempts to think about the Creation section both nowadays and in the past, along with possible pathways for its on-going – and essential – reinvention.
DOI: 10.6084/m9.figshare.5885611
title: Analysis of 18 bp Ins/Del at -2549 polymorphism of the vascular endothelial growth factor gene in patients with systemic lupus erythematosus

description: ABSTRACT Systemic lupus erythematosus (SLE) is considered an autoimmune disease characterized by the action of autoantibodies, which cause chronic inflammation in various tissues of the body. Considering the vascular endothelial growth factor (VEGF) participation in the development of inflammation, this study aimed to evaluate the frequency of the polymorphism at the -2549 position (Ins/Del 18pb, rs35569394) in patients with SLE and in healthy individuals. No statistical differences were found when comparing the allele and genotype frequencies between patients and controls, suggesting that there is no association between the studied polymorphism and the development of SLE.
DOI: 10.6084/m9.figshare.5885611.v1
title: Analysis of 18 bp Ins/Del at -2549 polymorphism of the vascular endothelial growth factor gene in patients with systemic lupus erythematosus

description: ABSTRACT Systemic lupus erythematosus (SLE) is considered an autoimmune disease characterized by the action of autoantibodies, which cause chronic inflammation in various tissues of the body. Considering the vascular endothelial growth factor (VEGF) participation in the development of inflammation, this study aimed to evaluate the frequency of the polymorphism at the -2549 position (Ins/Del 18pb, rs35569394) in patients with SLE and in healthy individuals. No statistical differences were found when comparing the allele and genotype frequencies between patients and controls, suggesting that there is no association between the studied polymorphism and the development of SLE.
DOI: 10.6084/m9.figshare.5885605
title: Diagnostic methods for identification of root-knot nematodes species from Brazil

description: ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil.
DOI: 10.6084/m9.figshare.5885605.v1
title: Diagnostic methods for identification of root-knot nematodes species from Brazil

description: ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil.
DOI: 10.6084/m9.figshare.5885599
title: The relation of encyclopedias with the indexes and the semantic Web: Organization and signification supports in postmodernity

description: Abstract The study aims to study the process of signification (or “semantization”) that occurs in cyberspace, especially through Wikipedia, the semantic web and Knowledge Databases as Freebase and DBpedia, to name the most important ones, modeling data with Resource Description Framework. Through documentary research review, we sought to understand this movement that builds, in cyberspace, a network or web of significations to be retrieved, activated and interpreted by machines and humans. From the theoretical corpus analysis, we can see the design of encyclopedia as Education, then as a repertoire of knowledge, in the classical world and as a reference work, in modernity. In postmodernity it gets a new plane of consistency, as semantic organization of knowledge and as a database. In cyberspace, contemporary indexes and Wikipedia are a corpus in continuous renewal and expansion and are interconnected by semantic metadata representation, which fill the corpus of knowledge databases readable by intelligent agents. These relationships reframe concepts of encyclopedia, indexes and even Semantics, showing machinic and hybrids assemblages of the organization and signification processes in postmodernity.
DOI: 10.6084/m9.figshare.5885590
title: Ultrasound spectroscopy as an alternative method to measure the physical-chemical constituents of buffalo milk

description: ABSTRACT: This study aimed to use ultrasound spectroscopy for the determination of the physical-chemical characteristics of buffalo milk and compare it to theinfrared method. Levels of fat, protein, lactose and non-fat solids (NFS) were determined in milk samples of 22 buffaloes (n = 383) with initial milk production of 6.97 ± 1.55 litres. The respective average results for the fat, protein, lactose and NFS of the individual samples were 6.31%, 3.81%, 4.99% and 9.75% for the infrared method-PO ANA 009 and 7.16%, 2.5%, 6.28% and 9.41% using ultrasound spectroscopy. There were significant differences (P&lt;0.0001) in the levels of all of the components analysed between the two methods studied. Results obtained in the analyses using the ultrasonic milk analyser (Ekomilk Total®) were different from those obtained by the infrared method-PO ANA 009 (ESALQ), but they showed a high positive correlation for fat (r =0.84108, P&lt;0.0001), moderate correlation for NFS(r= 0.71284 P = 0.0022), low correlation for lactose (r= 0.32197; P&lt;0.0001) and the absence of correlation for protein(r= -0.00284, P&lt;0.0001). Therefore, ultrasound spectroscopy can be used forthe determination of fat. For the other constituents of buffalo milk, in order to use the ultrasonic analyser, it is suggested that further studies should be conducted for technical and methodological adjustments.