DOI: 10.5061/dryad.82f5q
title: Data from: Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

description: Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance
 and resistance enhancing genes in wheat is a cost-effective and
 environmentally safe strategy for disease control. PCR-based markers and
 cytogenetic analysis with genomic in situ hybridisation were applied to
 identify alien chromatin in four genetically diverse populations of wheat
 (Triticum aestivum) lines incorporating chromosome segments from
 Thinopyrum intermedium and Secale cereale (rye). Out of 20 experimental
 lines, 10 carried Th. intermedium chromatin as T4DL*4Ai#2S translocations,
 while, unexpectedly, 7 lines were positive for alien chromatin (Th.
 intermedium or rye) on chromosome 1B. The newly described rye 1RS
 chromatin, transmitted from early in the pedigree, was associated with
 enhanced WSMV resistance. Under field conditions, the 1RS chromatin alone
 showed some resistance, while together with the Th. intermedium 4Ai#2S
 offered superior resistance to that demonstrated by the known resistant
 cultivar Mace. Most alien wheat lines carry whole chromosome arms, and it
 is notable that these lines showed intra-arm recombination within the 1BS
 arm. The translocation breakpoints between 1BS and alien chromatin fell in
 three categories: (i) at or near to the centromere, (ii) intercalary
 between markers UL-Thin5 and Xgwm1130 and (iii) towards the telomere
 between Xgwm0911 and Xbarc194. Labelled genomic Th. intermedium DNA
 hybridised to the rye 1RS chromatin under high stringency conditions,
 indicating the presence of shared tandem repeats among the cereals. The
 novel small alien fragments may explain the difficulty in developing
 well-adapted lines carrying Wsm1 despite improved tolerance to the virus.
 The results will facilitate directed chromosome engineering producing
 agronomically desirable WSMV-resistant germplasm.
description: WSMV disease scores raw
 dataLines were originally
 scored in 2002 for response to natural infection by WSMV at Hays, KS USA
 and Sidney, NE USA (Divis et al., 2006). Visual subjective assessment of
 the degree of chlorosis and plant stunting were used (Table 1 and
 footnotes). Selected lines were further evaluated in the field at the
 University of Nebraska Agricultural Research and Development Center, Mead,
 NE. In 2011 individual spikes from each line were harvested and used to
 seed at least 20, 1.2 m replicate rows of single plant-derived lines in
 September 2011 as a block surrounded by early planted wheat that served as
 a ‘green bridge’ for the development of WCM populations. Multiple
 plantings of the resistant cultivar Mace and the susceptible line Tomahawk
 were included as a controlPCR gels of markers in
 TableS1 and S2_IFirst of two files with
 images of gels used for scoring.PCR gels of markers in
 TableS1 and S2_IISecond of two files of
 gel images used for scoring.FISH_ExperimentTable with FISH experiments analysed for each line describedFISH_Experiment_Schedules_IFirst of two files with slides and probes for FISH experimentsFISH_Experiment_Schedules_IISecond of two files with slides and probes for FISH experimentsFISH experiments of line MilleniumZip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.Millenium.zipFISH experiments for line KS95H102Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.KS95H102.zipFISH experiment for line KS96HW10-1Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.KS96HW10-1.zipFISH experiments for line N02Y5003Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5003.zipFISH Experiments for line N02Y5096Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5096.zipFISH Experiments for line N02Y5018Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5018.zipFISH experiments for line N02Y5019Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5019.zipFISH experiments for N02Y5021Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5021.zipFISH experiments for line N02Y5025Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5025.zipFISH experiments for line N02Y5057Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5057.zipFISH experiments for line N02Y5075Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5075.zipFISH experiments for line N02Y5078Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5078.zipFISH experiments for line N02Y5082Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5082.zipFISH experiments for line N02Y5105Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5105.zipFISH experiments for line N02Y5106Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5106.zipFISH experiments for line N02Y5109Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5109.zipFISH experiments for line N02Y5117 MaceZip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5117 Mace.zipFISH experiments for line N02Y5121Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5121.zipFISH experiments for line N02Y5149Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5149.zipFISH experiments for line N02Y5154Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5154.zipFISH experiments for line N02Y5156Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y5156.zipFISH experiments for line N02Y5163Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detailN02Y5163.zipFISH experiments for line N02Y2016Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.N02Y2016.zip