cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.dfn2z35cf
title: Impact of the POLRMT inhibitor IMT1B on mitochondrial genome copy number in Caenorhabditis elegans

description: POLRMT is the dedicated mitochondrial RNA polymerase in metazoans and is
 essential for priming mitochondrial DNA (mtDNA) synthesis. Aberrant POLRMT
 causes mitochondrial dysfunction and has previously been linked to human
 metabolic disease. The small molecule IMT1B is an allosteric inhibitor of
 POLRMT, and its use in diverse model organisms is informative about
 various aspects of mtDNA synthesis and transcription. Previously, this
 drug has been shown to be effective in perturbing mtDNA gene expression in
 human cells and mice. Moreover, a leucine to phenylalanine substitution at
 position 813 of human POLRMT is predicted to disrupt interaction with the
 drug. However, the effect of the F813L mutation on the efficacy of POLRMT
 inhibition (POLRMTi) has not been rigorously tested. Here, we determined
 by multiple sequence alignment and phylogenetic analysis that this
 mutation in POLRMT is common among invertebrates, including the model
 nematode Caenorhabditis elegans. AlphaFold analyses of metazoan
 POLRMT folding suggest that the F813L substitution alters the
 physicochemical features of the IMT1B binding pocket. Finally, we find
 that IMT1B treatment of larval Caenorhabditis elegans has little impact on
 mtDNA copy number, suggesting that POLRMTi via IMT1B may not be effective
 in this model organism.
description: Quantitative PCR; AlphaFold modeling; Phylogenetics
description: # Impact of the POLRMT/polrmt-1 inhibitor IMT1B on mitochondrial genome
 copy number in Caenorhabditis elegans
 [https://doi.org/10.5061/dryad.dfn2z35cf](https://doi.org/10.5061/dryad.dfn2z35cf) **Corresponding investigator:** Samantha Lewis \[[samlewis@berkeley.edu](mailto:samlewis@berkeley.edu)] University of California, Berkeley **Co-Investigators:** Eve Kakudji, M.S. University of California, Berkeley ## Description of the data and file structure This dataset contains raw data, protocols, and analysis used to produce figures and conclusions associated with a poster presented at the American Society of Cell Biology conference in December 2024.  Multiple sequence alignments are provided as clustalw .aln files that can be opened in MEGA. Quantitative PCR data is provided as an Excel file. All other files are in Supplemental Information. Detailed description of the analysis methodology can be found in the Supplemental Information file 'Methods_details_Kakudji.docx'. Inputs to AlphaFold used to produce the structure comparisons between POLRMT/polrmt-1 across metazoan are provided in the Supplemental Information file 'Comparison of POLRMT structures via AlphaFold.docx'. The relevant poster is provided as a PDF. #### File: POLRMT alignment clustalw\.aln **Description:** Aligned protein sequences. #### File: Summary qPCR all **Description:** Compiled mtDNA qPCR data from 3 replicates and mtDNA copy number calculations. Sheet1: Calculation of absolute mtDNA copy number per cell normalized to single copy nuclear gene, from Delta Ct values. Sheet2: Calculation of fold change mtDNA per treatment, from Delta Ct values.
DOI: 10.60521/332643
title: Processed Gridded Bathymetry Data (EM302) acquired during E/V Nautilus expedition NA164 (2024)

description: This data set was acquired with a Kongsberg EM302 Multibeam Sonar during E/V Nautilus expedition NA164 conducted in 2024. These data files are of GeoTIFF (Raster) format and include Bathymetry data that were processed after acquisition. Data were acquired as part of the project: NOAA Ocean Exploration Cooperative Institute (OECI). Funding was provided by: NA19OAR4320072.
DOI: 10.60521/332644
title: Processed Gridded Acoustic Backscatter Data (EM302) acquired during E/V Nautilus expedition NA164 (2024)

description: This data set was acquired with a Kongsberg EM302 Multibeam Sonar during E/V Nautilus expedition NA164 conducted in 2024. These data files are of GeoTIFF (Raster) format and include Acoustic Backscatter data that were processed after acquisition. Data were acquired as part of the project: NOAA Ocean Exploration Cooperative Institute (OECI). Funding was provided by: NA19OAR4320072.
DOI: 10.11588/data/fspql4
title: The Abbott PanBio WHO emergency use listed, rapid, antigen-detecting point-of-care diagnostic test for SARS-CoV-2 - Evaluation of the accuracy and ease-of-use [Research Data]
title: Evaluation of the accuracy and ease-of-use of PanBio

description: This repository includes data from a prospective, multi-centre diagnostic accuracy study. The study was conducted at two sites in Germany, Heidelberg and Berlin. Following routine testing with reverse-transcriptase polymerase chain reaction (RT-PCR), a second study-exclusive swab was performed for Ag-RDT testing. Routine swabs were nasopharyngeal (NP) or combined NP/oropharyngeal (OP) whereas the study-exclusive swabs were NP. To evaluate performance, sensitivity and specificity were assessed overall and in predefined sub analyses accordingly to cycle-threshold values, days of symptoms, disease severity and study site. In addition, further data from the participants were collected during interviews.
description: R, 4.0.3
description: REDCap, 10.0.33
DOI: 10.6084/m9.figshare.30186121
title: Epigenetic age in blood and bones from deceased individuals: assessment of DNA methylation at the FHL2 gene by droplet digital PCR

description: Blood and bones are essential DNA resources in forensic contexts. We examined DNA methylation (DNAm) levels at the <i>FHL2</i> CpG site cg06639320 for age estimation purposes using droplet digital PCR (ddPCR) on DNA extracts from bones and blood samples of deceased Portuguese individuals. Blood samples from 56 individuals (11 women and 45 men, aged 24 to 91 years) and bone samples from 26 individuals (four women and 22 men, aged 26 to 80 years) were collected. The DNA was subjected to bisulphite conversion followed by ddPCR using dual-labelled probes targeting both methylated and unmethylated DNA sequences. Simple linear regression allowed us to obtain a strong correlation between DNAm levels and chronological age in blood samples (<i>R</i> = 0.789; <i>p</i> = 5.14 × 10<sup>−13</sup>), explaining 62% of age variance, as well as in bones (<i>R</i> = 0.849; <i>p</i> = 4.136 × 10<sup>−8</sup>), explaining 71% of age variance. The achieved mean absolute deviation (MAD) between chronological and predicted ages was 8.42 years (RMSE = 10.22) for blood and 6.09 years (RMSE = 7.07) for bones. Using ddPCR methodology, this study emphasizes the <i>FHL2</i> CpG site cg06639320 as well as blood and bone samples of deceased individuals for age estimation in forensic contexts.
DOI: 10.6084/m9.figshare.30186121.v1
title: Epigenetic age in blood and bones from deceased individuals: assessment of DNA methylation at the FHL2 gene by droplet digital PCR

description: Blood and bones are essential DNA resources in forensic contexts. We examined DNA methylation (DNAm) levels at the <i>FHL2</i> CpG site cg06639320 for age estimation purposes using droplet digital PCR (ddPCR) on DNA extracts from bones and blood samples of deceased Portuguese individuals. Blood samples from 56 individuals (11 women and 45 men, aged 24 to 91 years) and bone samples from 26 individuals (four women and 22 men, aged 26 to 80 years) were collected. The DNA was subjected to bisulphite conversion followed by ddPCR using dual-labelled probes targeting both methylated and unmethylated DNA sequences. Simple linear regression allowed us to obtain a strong correlation between DNAm levels and chronological age in blood samples (<i>R</i> = 0.789; <i>p</i> = 5.14 × 10<sup>−13</sup>), explaining 62% of age variance, as well as in bones (<i>R</i> = 0.849; <i>p</i> = 4.136 × 10<sup>−8</sup>), explaining 71% of age variance. The achieved mean absolute deviation (MAD) between chronological and predicted ages was 8.42 years (RMSE = 10.22) for blood and 6.09 years (RMSE = 7.07) for bones. Using ddPCR methodology, this study emphasizes the <i>FHL2</i> CpG site cg06639320 as well as blood and bone samples of deceased individuals for age estimation in forensic contexts.
DOI: 10.6084/m9.figshare.30882607
title: Development of an electrochemiluminescence assay to measure serum darbepoetin concentration in extremely preterm infants

description: The erythropoiesis stimulating agents (ESAs), erythropoietin (Epo) and darbepoetin (Darbe), are administered clinically to preterm infants to stimulate red cell production and decrease red blood cell transfusions. Darbe is longer acting compared to Epo, allowing for weekly administration; however, Darbe does not have a specific assay to allow for serum measurements. We developed a sensitive and specific electrochemiluminescent assay for future reliable measurement of serum and plasma Darbe concentrations in samples from preterm infants participating in the multicenter randomized Phase III Darbepoetin Trial to Improve Red Cell Mass and Neuroprotection in Preterm Infants. A multi-array electrochemiluminescence platform was used to develop an immunoassay to quantitatively measure Darbe concentrations in biological fluids. Capture and detection antibodies were identified, evaluated, and optimized. Assay sensitivity, intra- and inter-plate reproducibility, and specificity were assessed to verify the utility of the assay for the purpose of accurately measuring Darbe in these samples. A reliable and accurate assay to measure Darbe concentrations in serum or plasma was developed and validated on a clinically translatable platform. This method will be applied to quantify Darbe serum concentrations in extremely premature infants and to perform population pharmacokinetic analyses. <b>Clinical Trial Registration:</b>https://clinicaltrials.gov/study/NCT03169881. Identifier is NCT03169881.
DOI: 10.6084/m9.figshare.30882607.v1
title: Development of an electrochemiluminescence assay to measure serum darbepoetin concentration in extremely preterm infants

description: The erythropoiesis stimulating agents (ESAs), erythropoietin (Epo) and darbepoetin (Darbe), are administered clinically to preterm infants to stimulate red cell production and decrease red blood cell transfusions. Darbe is longer acting compared to Epo, allowing for weekly administration; however, Darbe does not have a specific assay to allow for serum measurements. We developed a sensitive and specific electrochemiluminescent assay for future reliable measurement of serum and plasma Darbe concentrations in samples from preterm infants participating in the multicenter randomized Phase III Darbepoetin Trial to Improve Red Cell Mass and Neuroprotection in Preterm Infants. A multi-array electrochemiluminescence platform was used to develop an immunoassay to quantitatively measure Darbe concentrations in biological fluids. Capture and detection antibodies were identified, evaluated, and optimized. Assay sensitivity, intra- and inter-plate reproducibility, and specificity were assessed to verify the utility of the assay for the purpose of accurately measuring Darbe in these samples. A reliable and accurate assay to measure Darbe concentrations in serum or plasma was developed and validated on a clinically translatable platform. This method will be applied to quantify Darbe serum concentrations in extremely premature infants and to perform population pharmacokinetic analyses. <b>Clinical Trial Registration:</b>https://clinicaltrials.gov/study/NCT03169881. Identifier is NCT03169881.
DOI: 10.15468/dl.y72dr2
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Actinidia kolomikta",
 "Country is Norway",
 "HasCoordinate is true"
 ]
}

 The dataset includes 15 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0049778-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.2mbg9t
title: Occurrence Download

description: A dataset containing 981 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (Brazil, Paraguay, Bolivia (Plurinational State of))",
 "DatasetKey is iNaturalist Research-grade Observations",
 "OccurrenceStatus is Present",
 "TaxonKey is Caiman latirostris",
 "Year 2000-2025"
 ]
}

 The dataset includes 981 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0049781-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.mck97n
title: Occurrence Download

description: A dataset containing 13747 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Chamaecrista mimosoides (L.) Greene"
 ]
}

 The dataset includes 13747 records from 235 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001829-250424135625364/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.z4teuh
title: Occurrence Download

description: A dataset containing 971836 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is United States of America",
 "OccurrenceStatus is Present",
 "TaxonKey is Bombus Latreille, 1802"
 ]
}

 The dataset includes 971836 records from 164 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001816-250424135625364/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.f3d98u
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Vicia sativa",
 "Country is Zimbabwe",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0024973-250811113504898/datasets/export for details.
DOI: 10.15468/dl.vj93pv
title: Occurrence Download

description: A dataset containing 6 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Rumex acetosella L.",
 "Country is India"
 ]
}

 The dataset includes 6 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010264-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fmns6a
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Holtbyrnia macrops Maul, 1957",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((45.6579 11.3446,44.9648 11.0757,44.9648 10.8496,51.6539 10.8496,51.6211 10.9131,51.7357 12.0497,51.4872 12.2937,50.7681 12.4846,50.3292 12.3424,50.0295 12.0332,49.0651 11.773,47.2785 11.6543,46.4751 11.2145,45.6579 11.3446))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0010265-250525065834625/datasets/export for details.
DOI: 10.15468/dl.akamee
title: Occurrence Download

description: A dataset containing 207 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is Australia",
 "GadmGid is AUS.10.56_1",
 "Geometry POLYGON((145.3 -37.7,145.7 -37.7,145.7 -37.4,145.3 -37.4,145.3 -37.7))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Gymnobelideus leadbeateri McCoy, 1867"
 ]
}

 The dataset includes 207 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017099-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6084/m9.figshare.29220161
title: Data_Zheng et al_submission_peatlandpocmaoc

description: peatland POC and MAOC
DOI: 10.6084/m9.figshare.29220161.v1
title: Data_Zheng et al_submission_peatlandpocmaoc

description: peatland POC and MAOC
DOI: 10.15468/dl.u4dkge
title: Occurrence Download

description: A dataset containing 29890 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Continent is Asia",
 "Country is Japan",
 "TaxonKey is Persicaria (L.) Mill.",
 "Year 1900-2025"
 ]
}

 The dataset includes 29890 records from 92 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017100-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ta7hhd
title: Occurrence Download

description: A dataset containing 43 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Specimen, Occurrence evidence)",
 "Country is Brazil",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Chalcopteryx Selys, 1853"
 ]
}

 The dataset includes 43 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017103-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15520141
title: Exploration of the electron density and bare nuclear potential of all isomers of W3-W30 from wdbase (within 1kcal/mol energy range from the putative minima)
DOI: 10.5281/zenodo.15520142
title: Exploration of the electron density and bare nuclear potential of all isomers of W3-W30 from wdbase (within 1kcal/mol energy range from the putative minima)
DOI: 10.15468/dl.9czxyg
title: Occurrence Download

description: A dataset containing 72067 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Lachnospiraceae"
 ]
}

 The dataset includes 72067 records from 539 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017097-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/12042951
title: Food delivery project management sytems
DOI: 10.15468/dl.jubtey
title: Occurrence Download

description: A dataset containing 7526 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Lactarius indigo (Schwein.) Fr."
 ]
}

 The dataset includes 7526 records from 68 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017104-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7mwqt6
title: Occurrence Download

description: A dataset containing 207 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Human Observation",
 "Country is Australia",
 "GadmGid is AUS.10.56_1",
 "Geometry POLYGON((145.3 -37.7,145.7 -37.7,145.7 -37.4,145.3 -37.4,145.3 -37.7))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Gymnobelideus leadbeateri McCoy, 1867"
 ]
}

 The dataset includes 207 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017106-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15581324
title: Hiawatha Glacier Active Source Seismic Data

description: Active source seismic data collected on Hiawatha Glacier in the 2022 SIIOS field season, with accompanying field notes.
DOI: 10.5281/zenodo.15581325
title: Hiawatha Glacier Active Source Seismic Data

description: Active source seismic data collected on Hiawatha Glacier in the 2022 SIIOS field season, with accompanying field notes.
DOI: 10.5281/zenodo.15581238
title: Data and code for: Distribution prediction of Myristica yunnanensis under climate change

description: This dataset includes species occurrence records, environmental variables, and MaxEnt outputs used in the manuscript.
DOI: 10.34804/supra.20240816572
title: A cationic water-soluble pillar[5]arene: synthesis and host–guest complexation with sodium 1-octanesulfonate
DOI: 10.60915/boehringer-figshare.29220170
title: Supplemental materials for DINAMO MONO manuscript

description: This is the supplemental material for the publication 'A comparison of SGLT2 or DPP-4 inhibitor monotherapy vs placebo for type 2 diabetes in adolescents vs young adults ' in the journal JES.
DOI: 10.60915/boehringer-figshare.29220170.v1
title: Supplemental materials for DINAMO MONO manuscript

description: This is the supplemental material for the publication 'A comparison of SGLT2 or DPP-4 inhibitor monotherapy vs placebo for type 2 diabetes in adolescents vs young adults ' in the journal JES.
DOI: 10.15468/dl.e8yzg3
title: Occurrence Download

description: A dataset containing 17 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Japan",
 "GadmGid is JPN.13_1",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Egeria densa Planch."
 ]
}

 The dataset includes 17 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017107-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.xzkh3h
title: Occurrence Download

description: A dataset containing 158888 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hexactinellida"
 ]
}

 The dataset includes 158888 records from 260 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017101-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.w5vuta
title: Occurrence Download

description: A dataset containing 140 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Camellia petelotii (Merr.) Sealy"
 ]
}

 The dataset includes 140 records from 26 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017108-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nrq44j
title: Occurrence Download

description: A dataset containing 482 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Occurrence evidence)",
 "GadmGid is one of (AUS.7.44_1, AUS.7.62_1)",
 "OccurrenceStatus is Present",
 "TaxonKey is Macropus Shaw, 1790",
 "Year 2020-2025"
 ]
}

 The dataset includes 482 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017109-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ankpse
title: Occurrence Download

description: A dataset containing 977 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rhabdotorrhinus leucocephalus (Vieillot, 1816)"
 ]
}

 The dataset includes 977 records from 39 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0017110-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.34740/kaggle/dsv/12043010
title: Peer-to-Peer Boliviano (BOB) Exchange Data