DOI: 10.22002/kc21m-pt371
title: Caulobacter crescentus

description: wild type CB15N. 4th bug of day, dual-axis dataset, lacy carbon. extended stationary phase, ATCC PYE formula, stressed cells
description: <strong>Tilt Series Date:</strong> 2004-06-24</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Dual Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> extended stationary phase, tapwater ATCC (PYE) media, wild-type CB15N</p>
<strong>Sample Preparation:</strong> Lacy carbon, 10nm gold on grids & in suspension, 100% humidity, -3 offset, 1 sec blot, 2 sec drain, gold on carbon & in cells</p>
  SAVE_Jun24t04p8.jpg: file associated to 3D Image #210 SAVE_Jun24t04s239.jpg: file associated to 3D Image #210 pdd2004-06-24-4_slicer5230.jpg: Unknown pdd2004-06-24-4_slicer5231.jpg: Unknown pdd2004-06-24-4_slicer5232.jpg: Unknown pdd2004-06-24-4_slicer5233.jpg: Unknown pdd2004-06-24-4_slicer5239.jpg: Unknown pdd2004-06-24-4_slicer5240.jpg: Unknown pdd2004-06-24-4_slicer5242.jpg: Unknown parallel filament pdd2004-06-24-4_slicer5244.jpg: Unknown circle-like object with rings inside
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/rawdata/</p>CC04Jun24e100t04a.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/rawdata/CC04Jun24e100t04a.mrc"
        > <i class="download icon"></i> Download </a></p>

        CC04Jun24e100t04b.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/rawdata/CC04Jun24e100t04b.mrc"
        > <i class="download icon"></i> Download </a></p>

        04Jun24t04.mrc,  Reconstruction (Pixel Size 2.4 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/3dimage_208/04Jun24t04.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ebina.rec,  Reconstruction (Pixel Size 2.4 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/3dimage_209/Ebina.rec"
        > <i class="download icon"></i> Download </a></p>

        Ebinb.rec,  Reconstruction (Pixel Size 2.4 nm) Notes for Full: Keywords:  .  extended stationary phase .  lacy carbon .  100 electrons per tilt series .  dual-axis .  CB15 .  GIFCCD, 943.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/3dimage_210/Ebinb.rec"
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        SAVE_Jun24t04p8.tif,  file associated to 3D Image #210, 2.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2004-06-24-4/file_304/SAVE_Jun24t04p8.tif"
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        SAVE_Jun24t04s239.tif,  file associated to 3D Image #210, 3.4 MB  
        
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