cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647828.v1
title: CIL:24815

description: Intracellular localization of GFP-GOLPH3 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutant, GFP-GOLPH3 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM. Thus, correct Golgi localization of Vps74 (see CIL# 24812 for GFP-Vps74 in sac1∆ vps74∆ mutant) and GOLPH3 requires synthesis of PtdIns4P in the Golgi by Pik1-Frq1 and maintenance of low PtdIns4P levels in non-Golgi membranes by the Sac1 lipid phosphatase. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1B GOLPH3/sac1∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1B include CIL#s 24815, 24812, 24813.
DOI: 10.6084/m9.figshare.647824
title: CIL:20151

description: Adapted from the original movie of a Balamuthia mandrillaris within a culture of monkey kidney cells (CIL:20152). The comparatively thick body of the ameba has a number of pseudopodia extending from it and a large prominent nucleus. The action of the pseudopodia is important in the feeding of the amebas. Their tips have a pointed, sharp, dense extension that has been seen to grow out, and move together with a snap (about 1 second), an action that is shown in the 2X enlargement and pointed out by the circles that have been added. This action is a typical movement of the tips of the pseudopodia. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs that demonstrate the puncture of the plasma membrane of a rounded, clear monkey kidney cell with a large, rounded, clear nucleus with an elongated dense nucleolus. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Adapted with permission. Details of preparation can be found to accompany CIL:19325.
DOI: 10.6084/m9.figshare.647825
title: CIL:24045

description: Starved Dictyostelium cells lacking cGMP-binding protein C (gbpC-; no guanylyl cyclase activity) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate toward the anode (right). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) In contrast, cathode-directed migration is strongly attenuated in wild-type cells in the same medium. Compare with wild-type control video (CIL# 24043) and other mutants lacking guanylyl cyclase activity (CIL#24044 and 24046). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647826.v1
title: CIL:24046

description: Starved Dictyostelium cells lacking both guanylyl cyclases (GCase A and GCase), but expressing GCase with an inactivated catalytic domain (sGCΔCat) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate continuously toward the anode (right). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) In contrast, cathode-directed migration is strongly attenuated in wild-type cells in the same medium. Compare with wild-type control video (CIL# 24043) and other mutants lacking guanylyl cyclase activity (CIL#24044, 24045). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647829.v1
title: CIL:24816

description: Intracellular localization of GFP-Vps74 in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vps74 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM. Control image for images showing ectopic expression of GFP-Vps74 with mutations in the sulfate-binding pocket (CIL# 24817, 24818). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A wild-type/sac1∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647822
title: CIL:13896

description: DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647823
title: CIL:13897

description: DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with glucose. This is the control image for CIL# 13896 in which constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Image is Fig 5C bottom left panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647824.v1
title: CIL:20151

description: Adapted from the original movie of a Balamuthia mandrillaris within a culture of monkey kidney cells (CIL:20152). The comparatively thick body of the ameba has a number of pseudopodia extending from it and a large prominent nucleus. The action of the pseudopodia is important in the feeding of the amebas. Their tips have a pointed, sharp, dense extension that has been seen to grow out, and move together with a snap (about 1 second), an action that is shown in the 2X enlargement and pointed out by the circles that have been added. This action is a typical movement of the tips of the pseudopodia. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs that demonstrate the puncture of the plasma membrane of a rounded, clear monkey kidney cell with a large, rounded, clear nucleus with an elongated dense nucleolus. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Adapted with permission. Details of preparation can be found to accompany CIL:19325.
DOI: 10.6084/m9.figshare.647825.v1
title: CIL:24045

description: Starved Dictyostelium cells lacking cGMP-binding protein C (gbpC-; no guanylyl cyclase activity) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate toward the anode (right). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) In contrast, cathode-directed migration is strongly attenuated in wild-type cells in the same medium. Compare with wild-type control video (CIL# 24043) and other mutants lacking guanylyl cyclase activity (CIL#24044 and 24046). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.
DOI: 10.6084/m9.figshare.647820
title: CIL:13882

description: Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13885). This image shows SPB localization of eGFP-Tem1 (eGFP, green) in metaphase cells. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1A, top panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.
DOI: 10.6084/m9.figshare.647821
title: CIL:13885

description: Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13882). This image shows SPB localization of eGFP-Tem1 (eGFP, green) in anaphase cells. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1B, top panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.
DOI: 10.6084/m9.figshare.647822.v1
title: CIL:13896

description: DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647823.v1
title: CIL:13897

description: DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with glucose. This is the control image for CIL# 13896 in which constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Image is Fig 5C bottom left panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.6084/m9.figshare.647819
title: CIL:13881

description: Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (CIL# 13880) but can also still localize to the SPB (this image). The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown. The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig S5D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S5 include CIL # 13880, 13881.
DOI: 10.6084/m9.figshare.647821.v1
title: CIL:13885

description: Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13882). This image shows SPB localization of eGFP-Tem1 (eGFP, green) in anaphase cells. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1B, top panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.
DOI: 10.6084/m9.figshare.647819.v1
title: CIL:13881

description: Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (CIL# 13880) but can also still localize to the SPB (this image). The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown. The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig S5D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S5 include CIL # 13880, 13881.
DOI: 10.6084/m9.figshare.647820.v1
title: CIL:13882

description: Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13885). This image shows SPB localization of eGFP-Tem1 (eGFP, green) in metaphase cells. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1A, top panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.
DOI: 10.6084/m9.figshare.647815
title: CIL:13871

description: Tem1 (green) primarily localizes to the daughter cell spindle pole body (SPB) during anaphase. Control image for CIL# 13870 in which Tem1 loading onto SPBs was severely affected in bfa1Δ cells. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647816
title: CIL:13878

description: During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 bfa1Δ cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image CIL# 13879. Image is Fig S4D, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S4 include CIL# 13877, 13876, 13879, 13878.
DOI: 10.6084/m9.figshare.647817
title: CIL:13879

description: During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image CIL# 13868. Image is Fig S4D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S4 include CIL# 13877, 13876, 13879, 13878.
DOI: 10.6084/m9.figshare.647818
title: CIL:13880

description: Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown. The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig S5D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S5 include CIL # 13880, 13881.
DOI: 10.6084/m9.figshare.647816.v1
title: CIL:13878

description: During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 bfa1Δ cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image CIL# 13879. Image is Fig S4D, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S4 include CIL# 13877, 13876, 13879, 13878.
DOI: 10.6084/m9.figshare.647814
title: CIL:13870

description: Tem1 (green) loading onto spindle pole bodies during anaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13871. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647815.v1
title: CIL:13871

description: Tem1 (green) primarily localizes to the daughter cell spindle pole body (SPB) during anaphase. Control image for CIL# 13870 in which Tem1 loading onto SPBs was severely affected in bfa1Δ cells. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647817.v1
title: CIL:13879

description: During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image CIL# 13868. Image is Fig S4D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S4 include CIL# 13877, 13876, 13879, 13878.
DOI: 10.6084/m9.figshare.647818.v1
title: CIL:13880

description: Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown. The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig S5D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S5 include CIL # 13880, 13881.
DOI: 10.6084/m9.figshare.647811
title: CIL:13741

description: Boi (red) localizes predominantly to apical cells in Drosophila wild-type germaria but not in germaria with UAS-boi[RNAi] expression (CIL#13742). Germ cells are identified using anti-Vasa (blue). Image is Fig 2D in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).
DOI: 10.6084/m9.figshare.647814.v1
title: CIL:13870

description: Tem1 (green) loading onto spindle pole bodies during anaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13871. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647812
title: CIL:13868

description: During late anaphase, Tem1 (green) localized to both spindle pole bodies (SPBs), but Bfa1 (red) was asymmetrically localized to the daughter SPB. DAPI (blue) and DIC (hidden) are also shown. Compare to metaphase image CIL# 13869. Image is Fig 6A, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647813
title: CIL:13869

description: Both Tem1 (green) and Bfa1 (red) are localized to both spindle pole bodies in metaphase. DAPI (blue) and DIC (hidden) are also shown. Compare to anaphase image CIL# 13868. Image is Fig 6A, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647813.v1
title: CIL:13869

description: Both Tem1 (green) and Bfa1 (red) are localized to both spindle pole bodies in metaphase. DAPI (blue) and DIC (hidden) are also shown. Compare to anaphase image CIL# 13868. Image is Fig 6A, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 6 include CIL# 13869, 13868, 13871, 13870.
DOI: 10.6084/m9.figshare.647810
title: CIL:13740

description: Boi (green) no longer localizes predominantly to apical cells in Drosophila boi[e] mutant germaria (compare with CIL# 13739). Germ cells are identified using anti-Vasa (blue). Image is Fig 2C in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).
DOI: 10.6084/m9.figshare.647811.v1
title: CIL:13741

description: Boi (red) localizes predominantly to apical cells in Drosophila wild-type germaria but not in germaria with UAS-boi[RNAi] expression (CIL#13742). Germ cells are identified using anti-Vasa (blue). Image is Fig 2D in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).
DOI: 10.6084/m9.figshare.647808
title: CIL:13457

description: Intracellular localization of GFP-GOLPH3 R171A, R174A mutant in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol (CIL# 13455) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 R171A R174A/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.
DOI: 10.6084/m9.figshare.647809
title: CIL:13739

description: Boi (green) localizes predominantly to apical cells in Drosophila wild-type germaria but not in boi[e] mutant germaria (CIL#13740). Germ cells are identified using anti-Vasa (blue). Image is Fig 2B in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).