cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/mpe94-ftf52
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-49/rawdata/</p>exsD-mini-cell_target_10.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-49/rawdata/exsD-mini-cell_target_10.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_10_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-49/3dimage_105524/exsD-mini-cell_target_10_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_10.mrc.mdoc,  63.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-49/rawdata/exsD-mini-cell_target_10.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1y2cg-tf026
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-32/rawdata/</p>exsD-mini-cell_target_34.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-32/rawdata/exsD-mini-cell_target_34.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_34_part121_10.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-32/3dimage_105378/exsD-mini-cell_target_34_part121_10.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_34.mrc.mdoc,  63.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-32/rawdata/exsD-mini-cell_target_34.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cw9dp-cwn49
title: MEF cells - Dynamin2 knockdown

description: Dynamin2 knocked down with retroviral delivery of shRNA to study mitochondrial division. grid info - DG2_grid1.
description: <strong>Tilt Series Date:</strong> 2018-11-27</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun Shik from Chan lab helped design shRNA and provided protocols for viral transduction into Chan lab's MEF cells. Shrawan and Danielle made the cell line. Shrawan FIB-milled and performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin (10min)</p>
<strong>Sample Preparation:</strong> Cells were treated with 10 µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-14/rawdata/</p>TS8-pt42.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-14/rawdata/TS8-pt42.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS7.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-14/rawdata/TS7.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS7_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-14/3dimage_102047/TS7_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ncy1e-gx383
title: Myxococcus xanthus MT299

description: BacM-mCherry correlated cryofLM/ECT imaging
description: <strong>Tilt Series Date:</strong> 2013-08-19</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu Finder</p>
  ycw2013-08-19-5_slicer9106.jpg: Myxococcus Xanthus .  filament bundle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-5/rawdata/</p>20130819_MT299_cryoLM_10005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-5/rawdata/20130819_MT299_cryoLM_10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130819_MT299_cryoLM_10005_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-5/3dimage_29770/20130819_MT299_cryoLM_10005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20130819_MT299_cryoLM_10005_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 267.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-5/3dimage_30215/20130819_MT299_cryoLM_10005_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.6dq76d
title: Occurrence Download

description: A dataset containing 374 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((-73.26724 -39.786,-73.26485 -39.78662,-73.25904 -39.78459,-73.25296 -39.7798,-73.2477 -39.7751,-73.24752 -39.76487,-73.24245 -39.75906,-73.24069 -39.75176,-73.24695 -39.74743,-73.2452 -39.74457,-73.23626 -39.74411,-73.23267 -39.73858,-73.23506 -39.73471,-73.24069 -39.73895,-73.24004 -39.73213,-73.23829 -39.72605,-73.23543 -39.7278,-73.22612 -39.73517,-73.22336 -39.73305,-73.21239 -39.73333,-73.19792 -39.73084,-73.18815 -39.72623,-73.18484 -39.72402,-73.18631 -39.72181,-73.17986 -39.7148,-73.16696 -39.70983,-73.15885 -39.70245,-73.16014 -39.69619,-73.16106 -39.69324,-73.16438 -39.69674,-73.16861 -39.70098,-73.17967 -39.69268,-73.18391 -39.6901,-73.16567 -39.68236,-73.16327 -39.67241,-73.15571 -39.67075,-73.1559 -39.66799,-73.16253 -39.66762,-73.16106 -39.6654,-73.16272 -39.66227,-73.16677 -39.66061,-73.16991 -39.64458,-73.16843 -39.63425,-73.15924 -39.61724,-73.13638 -39.61005,-73.12422 -39.60047,-73.12588 -39.59107,-73.13915 -39.59439,-73.15021 -39.60176,-73.15684 -39.60692,-73.16403 -39.6025,-73.17085 -39.60287,-73.17472 -39.60581,-73.18634 -39.6196,-73.18965 -39.62734,-73.18781 -39.63508,-73.18836 -39.64503,-73.19131 -39.65406,-73.18441 -39.65781,-73.18028 -39.66804,-73.18184 -39.67053,-73.18505 -39.67044,-73.18975 -39.67551,-73.19694 -39.67809,-73.20117 -39.68132,-73.20237 -39.68528,-73.20606 -39.69136,-73.20219 -39.69634,-73.20363 -39.69851,-73.21197 -39.70312,-73.21653 -39.71068,-73.21897 -39.70916,-73.22031 -39.71031,-73.21866 -39.71423,-73.21929 -39.71592,-73.22457 -39.71877,-73.23059 -39.71666,-73.23724 -39.71528,-73.23809 -39.71286,-73.24157 -39.71454,-73.24221 -39.71845,-73.24833 -39.72246,-73.25277 -39.72004,-73.25615 -39.72352,-73.25498 -39.72964,-73.26301 -39.73609,-73.27156 -39.73503,-73.27853 -39.74137,-73.28012 -39.74348,-73.27579 -39.74833,-73.26555 -39.75432,-73.26112 -39.75971,-73.26386 -39.77312,-73.27379 -39.78357,-73.26724 -39.786))",
 "OccurrenceStatus is Present",
 "TaxonKey is Cygnus melancoryphus",
 "Year 2015-2025"
 ]
}

 The dataset includes 374 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005782-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/x7y74-nwa41
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-5/rawdata/</p>20140308_YWC093_10009.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-5/rawdata/20140308_YWC093_10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140308_YWC093_10009_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-5/3dimage_40020/20140308_YWC093_10009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20140308_YWC093_10009_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-5/3dimage_40065/20140308_YWC093_10009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gmyde-1j562
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-3/rawdata/</p>20141022_YWC121_10003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-3/rawdata/20141022_YWC121_10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_10003.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-3/3dimage_47368/20141022_YWC121_10003.rec"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_10003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-3/3dimage_47443/20141022_YWC121_10003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.2q7e8r
title: Occurrence Download

description: A dataset containing 1366 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "PublishingCountry is Colombia",
 {
 "and" : [
 "Country is Colombia",
 {
 "not" : {
 "Issue" : [
 "is Country coordinate mismatch"
 ]
 }
 }
 ]
 }
 ]
 },
 {
 "and" : [
 "TaxonKey is one of (Espeletia argentea [GBIF Backbone Taxonomy])",
 "HasCoordinate is true"
 ]
 }
 ]
}

 The dataset includes 1366 records from 47 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005781-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/gy02m-95k03
title: E.cloacae-typeV-CdiA+

description: Type V secretion system
description: <strong>Tilt Series Date:</strong> 2014-03-03</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Drs. Davod Low and Chris Hayes, UC Santa Barbara</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning</p>
<strong>Sample Preparation:</strong> Blot time 3s, Blot off-set -4, 3 ul culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-3/rawdata/</p>atlas10003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-3/rawdata/atlas10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10003_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-3/3dimage_39518/atlas10003_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2014-03-03-3_slicer19344.png,  F7 chemo array, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2014-03-03-3_slicer19344.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.6nre5t
title: Occurrence Download

description: A dataset containing 173 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Rhincodon typus",
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "Year is greater than or equal to 2010",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON ((130 -50, 180 -50, 180 10, 130 10, 130 -50))"
 ]
}

 The dataset includes 173 records from 15 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005785-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/xbz83-r9p08
title: Myxococcus xanthus delta-tsaP

description: grid box YWC065
description: <strong>Tilt Series Date:</strong> 2013-12-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-8/rawdata/</p>20131227_YWC065_10008.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-8/rawdata/20131227_YWC065_10008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131227_YWC065_10008_part131_43.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-27-8/3dimage_36679/20131227_YWC065_10008_part131_43.rec"
        > <i class="download icon"></i> Download </a></p>

        20131227_YWC065_10008_part131_48.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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DOI: 10.22002/95w50-sbf65
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-15/rawdata/</p>20141022_YWC121_30002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
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        20141022_YWC121_30002.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
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        20141022_YWC121_30002_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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