DOI: 10.20350/digitalcsic/16541
title: Dataset for the paper "Hematological response to particle debris generated during wear–corrosion processes of CoCr surfaces modified with gra-phene oxide and hyaluronic acid for joint prostheses"

description: The dataset includes data on particle characterization of debris collected from wear-corrosion tests and blood analysis from rats after 30 days of inoculation. Particle size distribution of the debris was measured using a MasterSizer 3000 with laser diffraction technology. This device features a red He-Ne laser (wavelength: 632.8 nm), a blue LED laser (wavelength: 470 nm), and a Hydro SV small volume dispersion unit with stirring. Measurements were taken with laser obscuration set between 3% and 15%, and sample dispersion was achieved at 1200 rpm. Two debris samples were analyzed, each with three measurements. The dataset contains six files, each with two columns labeled "size" and "volume."Blood analysis was conducted prior to euthanizing the animals in a CO2 chamber, with 1.5 mL of intracardiac blood extracted. Serum samples were prepared by centrifugation at 3,000 x g for 15 minutes, then separated and stored in 1.5 mL Eppendorf tubes in a cold chamber for transport to a laboratory. Hematological analysis was performed by UNILABS (Madrid, Spain) using a Sysmex Kx-21n hematology autoanalyzer (Sysmex Corporation, Kobe, Japan). The dataset file consists of 21 columns, detailing results by treatment group (Serum control, Hyaluronic acid, and Graphene) and particle concentrations (0.63 mg/ml, 1.83 mg/ml, 1.65 mg/ml, and 2.35 mg/ml). Hematological parameters in the dataset include hemoglobin (Hgb), hematocrit (Hct), white blood cell (WBC) count, red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and platelet (PLT) count. Biochemical parameters measured in serum samples are creatinine (Crea), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), total protein (TP), triglycerides (TG), and cholesterol (CHO). Coagulation parameters include prothrombin time (PT), thromboplastin time (TT), and fibrinogen (F).
description: [EN] This is the experimental dataset used in the paper "Hematological response to particle debris generated during wear–corrosion processes of CoCr surfaces modified with graphene oxide and hyaluronic acid for joint prostheses". The paper includes the results of the potential biological responses and consequences of wear-corrosion processes associated with CoCr surfaces modified with graphene oxide and hyaluronic acid (CoCr-GO-HA), through in vitro (macrophage cultures) and in vivo (rat models) assays. Once the graphene oxide functionalized with hyaluronic acid was deposited on CoCr surfaces, wear-corrosion tests were performed by applying a 5N load at a rotational speed of 120 rpm over 30,000 m (682,000 cycles) in an aqueous solution of 3 g/L hyaluronic acid (HA), continuously recirculated during the experiment. The generated particles were collected and redispersed in 0.9% NaCl at concentrations of 0.63 mg/mL, 1.65 mg/mL, 1.83 mg/mL, and 2.35 mg/mL. The particles were intra-articularly injected into the knees of rats. This dataset includes the results of the wear particle characterization and the hematological analysis and biochemical parameters, 30 days after intra-articular knee injection in rats. Whole-blood hematological parameters included hemoglobin (Hgb), hematocrit (Hct), white blood cell (WBC) count, red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and platelet (PLT) count.
description: [ES] Este es el conjunto de datos experimentales utilizado en el artículo "Respuesta hematológica a los desechos de partículas generados durante los procesos de desgaste-corrosión de superficies de CoCr modificadas con óxido de grafeno y ácido hialurónico para prótesis articulares". El artículo incluye los resultados de las posibles respuestas biológicas y consecuencias de los procesos de desgaste-corrosión asociados con superficies de CoCr modificadas con óxido de grafeno y ácido hialurónico (CoCr-GO-HA), mediante ensayos in vitro (cultivos de macrófagos) e in vivo (modelos de ratas). Una vez depositado el óxido de grafeno funcionalizado con ácido hialurónico sobre superficies de CoCr, se realizaron ensayos de desgaste-corrosión aplicando una carga de 5N a una velocidad de rotación de 120 rpm durante 30,000 m (682,000 ciclos) en una solución acuosa 3 g/L de ácido hialurónico (HA), recirculada continuamente durante el experimento. Las partículas generadas se recogieron y se redispersaron en NaCl al 0.9%, a concentraciones de 0.63 mg/mL, 1.65 mg/mL, 1.83 mg/mL y 2.35 mg/mL. Las partículas fueron inyectadas intra-articularmente en rodilla de ratas. Este conjunto de datos incluye los resultados de la caracterización de partículas generadas durante los experimentos de desgaste-corrosión y los análisis hematológicos y parámetros bioquímicos después de 30 días de la inyección intraarticular en las rodillas de las ratas. Los parámetros hematológicos de sangre completa incluyeron hemoglobina (Hgb), hematocrito (Hct), conteo de glóbulos blancos (WBC), conteo de glóbulos rojos (RBC), volumen corpuscular medio (MCV), concentración media de hemoglobina corpuscular (MCHC) y conteo de plaquetas (PLT).