DOI: 10.5061/dryad.jdfn2z3mh
title: Data from: A new species of Periglandula symbiotic with the morning glory Ipomoea tricolor

description: Many morning glories in the family Convolvulaceae contain ergot alkaloids,
 which are important agricultural and pharmaceutical compounds produced
 exclusively by fungi. In the few morning glories that have been
 investigated in detail, the ergot alkaloids are associated with the
 presence of a symbiotic fungus from the family Clavicipitaceae. The genus
 Periglandula was erected in the family Clavicipitaceae in 2011 for two
 species of morning glory symbionts. Biochemical and limited sequence data
 indicate that Ipomoea tricolor, a commonly cultivated morning glory from
 Mexico, contains a Periglandula species but the fungus is uncharacterized,
 and no signs of it have ever been detected in the plant. Our goal was to
 isolate and characterize this fungus. We isolated the fungus in pure
 culture, characterized it morphologically, and prepared sufficient DNA
 from it to sequence its genome. Phylogenetic analyses based on multiple
 genes indicate that the symbiont of I. tricolor is distinct from, but
 related to, the two described species of Periglandula previously observed
 in other species of morning glories.
description: The genome of the fungal symbiont of <em>Ipomoea
 tricolor</em> was sequenced (GenBank accession JBIMFC000000000) from
 DNA obtained from an axenic culture. Sequence reads were obtained by
 Illumina NextSeq technology at the West Virginia University and Marshall
 University Genomics Core facilities and assembled with SPAdes version
 3.15.4. Homologs of the following loci were extracted from the genome of
 the symbiont of<em> I. tricolor</em> for phylogenetic analyses
 based on identity with homologs from the <em>Periglandula
 ipomoeae</em> genome: γ-actin (<em>actG</em>), β-tubulin
 (<em>tubB</em>), mitochondrial ATPase 6
 (<em>atp6</em>), largest subunit of RNA polymerase II
 (<em>rpbA</em>), dimethylallyltryptophan synthase
 (<em>dmaW</em>), and the ribosomal internal transcribed spacer
 region (ITS). Sequences were aligned by MUSCLE as found in MEGA X (Kumar
 et al. 2018) and trimmed by eye. Maximum likelihood analyses with 1000
 bootstrap replications were performed on ITS, <em>dmaW</em>,
 and concatenated <em>actG</em>, <em>tubB</em>,
 <em>atp6</em>, and <em>rpbA</em> loci. Those loci
 were selected for further analysis because they were available from both
 described <em>Periglandula</em> species––<em>P.
 ipomoeae</em>, whose genome was sequenced, and <em>P.
 turbinae</em> whose genome had not been sequenced and for which only
 limited sequence data were available. For the concatenated
 <em>actG</em>, <em>tubB</em>,
 <em>atp6</em>, and <em>rpbA</em> data set, we
 included members of each of the genera in the Clavicipitaceae that had
 sequenced genomes in which each of the four selected loci aligned well.
 Since multiple species in the genera <em>Epichloë</em> and
 <em>Metarhizium</em> met these criteria,
 three-well-characterized members of each of those genera were included.
 For ITS and <em>dmaW</em> trees, sequences from a greater
 number of undescribed <em>Periglandula</em> species were
 available in GenBank and thus were included in those analyses. Additional
 members of the Hypocreales were included as outgroups. Maximum likelihood
 analyses were conducted in MEGA X (Kumar et al. 2018) with general time
 reversible model for protein-coding sequences and the Tamura-Nei model for
 the internal transcribed spacer. Analyses were bootstrapped 1000 times,
 and the tree with the highest log likelihood is presented for each
 analysis. Kumar S, Stecher G, Li M, Knyaz C, Tamura K.
 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing
 platforms. Molec Biol Evol. 35(6):1547–1549.
description: # Alignments and sequence data supporting "A new species of
 Periglandula symbiotic with the morning glory Ipomoea tricolor"
 [https://doi.org/10.5061/dryad.jdfn2z3mh](https://doi.org/10.5061/dryad.jdfn2z3mh) ## Description of the data and file structure Data file contains DNA sequence alignments used as the foundations of phylogenetic trees appearing in Figure 3, 4, and 5 of Hazel and Panaccione, "A new species of *Periglandula* symbiotic with the morning glory *Ipomoea tricolor."*  Data are in FASTA format and ready to be copied and pasted for phylogenetic analyses.  Title line for each sequence contains organism name, locus name, and GenBank accession number. The sole exception to this naming pattern is a concatenated, four-gene data set, in which case the alignment data are immediately followed by individual trimmed sequence data labeled with organism name, locus, name and GenBank accession number.   ### Files and variables #### File: Hazel\_and\_Panaccione\_Alignments\_for\_Dryad\_as\_text.txt **Description:** Text document containing all relevant DNA sequence data to conduct phylogenetic analyses.  ## Access information Other publicly accessible locations of the data: * Raw data (prior to trimming and alignment) are available at GenBank (ncbi.nlm.nih.gov) Data was derived from the following sources: * Original data from the investigators as well as data downloaded from GenBank (ncbi.nlm.nih.gov).  GenBank accession numbers for data are provided in the data set.