cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20018211.v1
title: Study of four Neotropical species of tree crickets Oecanthus Serville, 1831 (Orthoptera, Gryllidae) using cytogenetic and molecular markers

description: Abstract Karyotypes in the worldwide subfamily Oecanthinae show variations in diploid number, chromosome morphology, and sex-chromosome system. This study described the chromosome set and phylogenetic relationships of four Neotropical species, Oecanthus lineolatus, O. valensis, O. pallidus, and O. pictus. We used classical cytogenetics and Bayesian Inference for phylogenetic reconstruction, using the mitochondrial genes COI, 12S rRNA, and 16S rRNA; and analyzed the phylogenetic patterns of changes in chromosome numbers, using ChromEvol. We observed differences in chromosome number among species and two different sex-chromosome systems. Oecanthus pictus showed 2n = 21, X0♂/22, XX♀; O. lineolatus, 2n = 20, XY♂/XX♀; and O. valensis and O. pallidus, 2n = 18, XY♂/XX♀. The karyotype of Oecanthus was asymmetric, one group with large chromosomes and variation in heterochromatin distribution, and another with small acrocentric chromosomes. The phylogenetic tree recovered two main groups: one with the Palearctic species and another with species from different bioregions, but with low posterior probability. The Neotropical species grouped separately, O. valensis and O. pictus with Nearctic and Ethiopian species, and O. pallidus and O. lineolatus in another, well-supported clade. Together, the phylogenic and chromosome data suggest descending dysploidy events during the evolution of the group.
DOI: 10.6084/m9.figshare.20018210
title: Interpopulation variation of transposable elements of the hAT superfamily in Drosophila willistoni (Diptera: Drosophilidae): in-situ approach

description: Abstract Transposable elements are abundant and dynamic part of the genome, influencing organisms in different ways through their presence or mobilization, or by acting directly on pre- and post-transcriptional regulatory regions. We compared and evaluated the presence, structure, and copy number of three hAT superfamily transposons (hobo, BuT2, and mar) in five strains of Drosophila willistoni species. These D. willistoni strains are of different geographical origins, sampled across the north-south occurrence of this species. We used sequenced clones of the hAT elements in fluorescence in-situ hybridizations in the polytene chromosomes of three strains of D. willistoni. We also analyzed the structural characteristics and number of copies of these hAT elements in the 10 currently available sequenced genomes of the willistoni group. We found that hobo, BuT2, and mar were widely distributed in D. willistoni polytene chromosomes and sequenced genomes of the willistoni group, except for mar, which is restricted to the subgroup willistoni. Furthermore, the elements hobo, BuT2, and mar have different evolutionary histories. The transposon differences among D. willistoni strains, such as variation in the number, structure, and chromosomal distribution of hAT transposons, could reflect the genomic and chromosomal plasticity of D. willistoni species in adapting to highly variable environments.
DOI: 10.6084/m9.figshare.20018210.v1
title: Interpopulation variation of transposable elements of the hAT superfamily in Drosophila willistoni (Diptera: Drosophilidae): in-situ approach

description: Abstract Transposable elements are abundant and dynamic part of the genome, influencing organisms in different ways through their presence or mobilization, or by acting directly on pre- and post-transcriptional regulatory regions. We compared and evaluated the presence, structure, and copy number of three hAT superfamily transposons (hobo, BuT2, and mar) in five strains of Drosophila willistoni species. These D. willistoni strains are of different geographical origins, sampled across the north-south occurrence of this species. We used sequenced clones of the hAT elements in fluorescence in-situ hybridizations in the polytene chromosomes of three strains of D. willistoni. We also analyzed the structural characteristics and number of copies of these hAT elements in the 10 currently available sequenced genomes of the willistoni group. We found that hobo, BuT2, and mar were widely distributed in D. willistoni polytene chromosomes and sequenced genomes of the willistoni group, except for mar, which is restricted to the subgroup willistoni. Furthermore, the elements hobo, BuT2, and mar have different evolutionary histories. The transposon differences among D. willistoni strains, such as variation in the number, structure, and chromosomal distribution of hAT transposons, could reflect the genomic and chromosomal plasticity of D. willistoni species in adapting to highly variable environments.
DOI: 10.6084/m9.figshare.20018211
title: Study of four Neotropical species of tree crickets Oecanthus Serville, 1831 (Orthoptera, Gryllidae) using cytogenetic and molecular markers

description: Abstract Karyotypes in the worldwide subfamily Oecanthinae show variations in diploid number, chromosome morphology, and sex-chromosome system. This study described the chromosome set and phylogenetic relationships of four Neotropical species, Oecanthus lineolatus, O. valensis, O. pallidus, and O. pictus. We used classical cytogenetics and Bayesian Inference for phylogenetic reconstruction, using the mitochondrial genes COI, 12S rRNA, and 16S rRNA; and analyzed the phylogenetic patterns of changes in chromosome numbers, using ChromEvol. We observed differences in chromosome number among species and two different sex-chromosome systems. Oecanthus pictus showed 2n = 21, X0♂/22, XX♀; O. lineolatus, 2n = 20, XY♂/XX♀; and O. valensis and O. pallidus, 2n = 18, XY♂/XX♀. The karyotype of Oecanthus was asymmetric, one group with large chromosomes and variation in heterochromatin distribution, and another with small acrocentric chromosomes. The phylogenetic tree recovered two main groups: one with the Palearctic species and another with species from different bioregions, but with low posterior probability. The Neotropical species grouped separately, O. valensis and O. pictus with Nearctic and Ethiopian species, and O. pallidus and O. lineolatus in another, well-supported clade. Together, the phylogenic and chromosome data suggest descending dysploidy events during the evolution of the group.
DOI: 10.6084/m9.figshare.20018214
title: Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

description: Abstract Bacillus thuringiensis BR145 isolated from a soybean field in Southern Brazil showed toxicity against two important insect pests from soybean crop, Helicoverpa armigera, and Chrysodeixis includens, with LC50 0.294 µg.cm-2 and 0.277 µg.cm-2, respectively. We analyzed the genome of this strain through sequences obtained by Next Generation DNA Sequencing and de novo assembly. The analysis of the genome revealed insecticidal genes cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Ab, cyt1, and vip3Aa, suggesting the use of this strain in new strategies of biological control.
DOI: 10.6084/m9.figshare.20018214.v1
title: Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

description: Abstract Bacillus thuringiensis BR145 isolated from a soybean field in Southern Brazil showed toxicity against two important insect pests from soybean crop, Helicoverpa armigera, and Chrysodeixis includens, with LC50 0.294 µg.cm-2 and 0.277 µg.cm-2, respectively. We analyzed the genome of this strain through sequences obtained by Next Generation DNA Sequencing and de novo assembly. The analysis of the genome revealed insecticidal genes cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Ab, cyt1, and vip3Aa, suggesting the use of this strain in new strategies of biological control.
DOI: 10.6084/m9.figshare.19945310.v1
title: Supporting Data 5 (Data S5): Raw LxCxE amino acid sequences

description: Amino acid sequences for 28 Viridiplantae representative species encoding at least 1 LxCxE motif
DOI: 10.6084/m9.figshare.20018216
title: Molecular and morphometric analysis of nominal Brachidontes exustus (Mollusca, Mytilidae) in Brazilian waters

description: Abstract Brachidontes exustus (Mollusca, Mytilidae) is mainly distributed in Central America, where it has been recognized as a _lataforma species. This study aimed to determine whether B. exustus extends beyond the Amazon Barrier and southward along the Brazilian West Atlantic coast. Mitochondrial genes coding for cytochrome-c oxidase, subunit I (COI) and 16S subunit of ribosomal _lataforma__ cid (16S rRNA) were analyzed with _lata parameters on Brazilian populations (Salvador, Arraial do Cabo and Fernando de Noronha) of scorched mussels previously recorded as B. exustus. Multivariate morphometric _latafor showed partial discrimination of species. Molecular _latafor confirmed B. exustus at Salvador, a population highly similar to Cartagena (Colombia), both belonging to the Atlantic Clade of the B. exustus complex. This fact adds evidence to the idea of the Amazon outflow as a semipermeable barrier. In the southeast of Brazil, B. exustus was not found; instead, B. darwinianus is the species represented at Arraial do Cabo (state of Rio de Janeiro), associated with brackish _lataf. Scorched mussels from Fernando de Noronha are most closely related to B. puniceus from Cape Verde with 4.4% differentiation. Demonstrating an independent evolutionary history since at least the beginning of the Pleistocene, its proposed new name is B. noronhensis.
DOI: 10.6084/m9.figshare.20018216.v1
title: Molecular and morphometric analysis of nominal Brachidontes exustus (Mollusca, Mytilidae) in Brazilian waters

description: Abstract Brachidontes exustus (Mollusca, Mytilidae) is mainly distributed in Central America, where it has been recognized as a _lataforma species. This study aimed to determine whether B. exustus extends beyond the Amazon Barrier and southward along the Brazilian West Atlantic coast. Mitochondrial genes coding for cytochrome-c oxidase, subunit I (COI) and 16S subunit of ribosomal _lataforma__ cid (16S rRNA) were analyzed with _lata parameters on Brazilian populations (Salvador, Arraial do Cabo and Fernando de Noronha) of scorched mussels previously recorded as B. exustus. Multivariate morphometric _latafor showed partial discrimination of species. Molecular _latafor confirmed B. exustus at Salvador, a population highly similar to Cartagena (Colombia), both belonging to the Atlantic Clade of the B. exustus complex. This fact adds evidence to the idea of the Amazon outflow as a semipermeable barrier. In the southeast of Brazil, B. exustus was not found; instead, B. darwinianus is the species represented at Arraial do Cabo (state of Rio de Janeiro), associated with brackish _lataf. Scorched mussels from Fernando de Noronha are most closely related to B. puniceus from Cape Verde with 4.4% differentiation. Demonstrating an independent evolutionary history since at least the beginning of the Pleistocene, its proposed new name is B. noronhensis.
DOI: 10.6084/m9.figshare.20018219
title: Investigation of changes in protein stability and substrate affinity of 3CL-protease of SARS-CoV-2 caused by mutations

description: Abstract 3CLpro of SARS-CoV-2 is one of the enzymes required for the replication process of the virus responsible for the COVID-19 pandemic. In this study, changes in protein stability and substrate affinity caused by mutations were investigated to stir the development of potent inhibitors. Sequence data of samples were obtained from the NCBI Virus database. Mutation analyses were performed with RDP4 and MegaX. 3CLpro tertiary models were created using Robetta. Molecular docking for peptidomimetic substrate and inhibitor ligand was done with Autodock v4.2 and Haddock v2.4. Protein stability analysis was performed using mCSM stability and DynaMut2. Twenty-four missense mutations in 3CLpro were identified in this study. Changes in the 3CLpro structure induced by the mutations Met49Thr, Leu167Ser, and Val202Ala resulted in significant levels of instability (-2.029,-2.612,-2.177 kcal.mol-1, respectively). The lowest interaction energy for substrate was -58.7 kcal.mol-1 and -62.6 kcal.mol-1 in wild-type and mutant, respectively. The lowest docking energy for ligand was -6.19 and -9.52 kcal.mol-1 for wild-type and mutant, respectively. This study reports for the first time that mutations cause increased substrate affinity of 3CLpro from SARS-CoV-2. This research provides important data for the development of potent peptidomimetic inhibitors for the treatment of COVID-19.
DOI: 10.6084/m9.figshare.20018219.v1
title: Investigation of changes in protein stability and substrate affinity of 3CL-protease of SARS-CoV-2 caused by mutations

description: Abstract 3CLpro of SARS-CoV-2 is one of the enzymes required for the replication process of the virus responsible for the COVID-19 pandemic. In this study, changes in protein stability and substrate affinity caused by mutations were investigated to stir the development of potent inhibitors. Sequence data of samples were obtained from the NCBI Virus database. Mutation analyses were performed with RDP4 and MegaX. 3CLpro tertiary models were created using Robetta. Molecular docking for peptidomimetic substrate and inhibitor ligand was done with Autodock v4.2 and Haddock v2.4. Protein stability analysis was performed using mCSM stability and DynaMut2. Twenty-four missense mutations in 3CLpro were identified in this study. Changes in the 3CLpro structure induced by the mutations Met49Thr, Leu167Ser, and Val202Ala resulted in significant levels of instability (-2.029,-2.612,-2.177 kcal.mol-1, respectively). The lowest interaction energy for substrate was -58.7 kcal.mol-1 and -62.6 kcal.mol-1 in wild-type and mutant, respectively. The lowest docking energy for ligand was -6.19 and -9.52 kcal.mol-1 for wild-type and mutant, respectively. This study reports for the first time that mutations cause increased substrate affinity of 3CLpro from SARS-CoV-2. This research provides important data for the development of potent peptidomimetic inhibitors for the treatment of COVID-19.
DOI: 10.6084/m9.figshare.19798981.v1
title: Supplementary Data 3 (Data S3): GO enrichment of LxCxE proteins Arabidopsis orthologs

description: This item contains datasets for Gene Ontology (Biological Process category) for 28 land plant, charophyte and chlorophyte algae species using the Arabidopsis orthologs of predicted LxCxE containing proteins. <br> Files are .csv and contain 3 columns: - GO Biological Process process -FDR-adjusted pvalue -Arabidopsis IDs contained in the enriched GO term
DOI: 10.6084/m9.figshare.19945310.v2
title: Supporting Data 6 (Data S6): Raw LxCxE amino acid sequences

description: Amino acid sequences for 28 Viridiplantae representative species encoding at least 1 LxCxE motif
DOI: 10.5281/zenodo.6620892
title: sed_catalogs

description: SED catalogs
DOI: 10.5281/zenodo.6620891
title: sed_catalogs

description: SED catalogs
DOI: 10.6084/m9.figshare.20018247
title: Women's meal frequency and nutritional and health profiles

description: OBJECTIVE: To verify whether meal frequency is associated with the nutritional and health profiles of women attending a health-promoting service. METHODS: This cross-sectional study included users of an Academia da Cidade in Belo Horizonte, Minas Gerais from August 2009 to December 2011. The following were collected: socioeconomic, demographic, health profile, anthropometric, and dietary data. Meal frequency was given by the self-reported number of daily meals and considered appropriate when &gt;5. Poisson regression with a robust variance was performed with a significance level of 5% (p&lt;0.05). RESULTS: The sample consisted of 342 females with a mean age of 50.6±13.7 years and a per capita income of R$ 500.0 (85.0; 4,666.7); 72.5% were adults. They had a median of 4 (1.0; 7.0) meals per day and 52.0% had fewer than five meals a day. Inadequate meal frequency was associated with high blood pressure (PR: 0.71; 95%CI: 0.56, 0.90; p=0.005) and constipation (PR: 0.77; 95%CI: 0.61, 0.98; p=0.040); inappropriate intakes of water (PR: 0.74; 95%CI: 0.59, 0.93; p=0.009), sugar (PR: 0.79; 95%CI: 0.63, 0.98; p=0,036), fruits (PR: 0.79; 95%CI: 0.63, 0.98; p=0.036), and non-starchy vegetables (PR: 0.74; 95%CI: 0.59, 0.94; p=0.012); and preference for whole milk (PR: 0.72; 95%CI: 0.57, 0.89; p=0.003). CONCLUSION: Inadequate daily meal frequency was associated with worse nutritional and health profiles, highlighting the importance of pertinent nutrition intervention.
DOI: 10.6084/m9.figshare.20018247.v1
title: Women's meal frequency and nutritional and health profiles

description: OBJECTIVE: To verify whether meal frequency is associated with the nutritional and health profiles of women attending a health-promoting service. METHODS: This cross-sectional study included users of an Academia da Cidade in Belo Horizonte, Minas Gerais from August 2009 to December 2011. The following were collected: socioeconomic, demographic, health profile, anthropometric, and dietary data. Meal frequency was given by the self-reported number of daily meals and considered appropriate when &gt;5. Poisson regression with a robust variance was performed with a significance level of 5% (p&lt;0.05). RESULTS: The sample consisted of 342 females with a mean age of 50.6±13.7 years and a per capita income of R$ 500.0 (85.0; 4,666.7); 72.5% were adults. They had a median of 4 (1.0; 7.0) meals per day and 52.0% had fewer than five meals a day. Inadequate meal frequency was associated with high blood pressure (PR: 0.71; 95%CI: 0.56, 0.90; p=0.005) and constipation (PR: 0.77; 95%CI: 0.61, 0.98; p=0.040); inappropriate intakes of water (PR: 0.74; 95%CI: 0.59, 0.93; p=0.009), sugar (PR: 0.79; 95%CI: 0.63, 0.98; p=0,036), fruits (PR: 0.79; 95%CI: 0.63, 0.98; p=0.036), and non-starchy vegetables (PR: 0.74; 95%CI: 0.59, 0.94; p=0.012); and preference for whole milk (PR: 0.72; 95%CI: 0.57, 0.89; p=0.003). CONCLUSION: Inadequate daily meal frequency was associated with worse nutritional and health profiles, highlighting the importance of pertinent nutrition intervention.
DOI: 10.6084/m9.figshare.20018249
title: Effect of weight and body composition changes on waist measurement of severely obese women receiving carbohydrate-restricted diet

description: OBJECTIVE: To evaluate the effect of weight and body composition changes on waist measurement of severely obese women receiving a low-carbohydrate diet for a short-term. METHODS: Nineteen severely obese women divided into an intervention and a control group received a low-carbohydrate diet and a conventional diet, respectively, both with 1,200kcal, for seven days. Anthropometric measurements such as weight, body mass index, arm circumference, waist circumference (at the navel), two proposed measurements of waist circumference (waist circumference 1, 10cm above the navel; and waist circumference 2, 20cm above the navel), hip circumference; and body composition (lean mass and fat mass) were performed at baseline and end of the study. RESULTS: There was significant reduction of all variables in the intervention group (p&lt;0.01), but the control group had no change in waist circumference (p=0.06) and hip circumference (p=0.36). Patients of the intervention group presented greater weight loss (4.4kg, p&lt;0.01) and reduction of body mass index, waist circumference, waist circumference 1, waist circumference 2 (p&lt;0.01), and fat mass (p=0.04) than the control group. CONCLUSION: The weight loss and body composition changes were greater in the low-carbohydrate group than in the conventional-diet group, and they had a greater impact on waist measurements. The proposed measurements (waist circumference 1 and waist circumference 2) were sensitive to weight reduction in both groups.
DOI: 10.6084/m9.figshare.20018249.v1
title: Effect of weight and body composition changes on waist measurement of severely obese women receiving carbohydrate-restricted diet

description: OBJECTIVE: To evaluate the effect of weight and body composition changes on waist measurement of severely obese women receiving a low-carbohydrate diet for a short-term. METHODS: Nineteen severely obese women divided into an intervention and a control group received a low-carbohydrate diet and a conventional diet, respectively, both with 1,200kcal, for seven days. Anthropometric measurements such as weight, body mass index, arm circumference, waist circumference (at the navel), two proposed measurements of waist circumference (waist circumference 1, 10cm above the navel; and waist circumference 2, 20cm above the navel), hip circumference; and body composition (lean mass and fat mass) were performed at baseline and end of the study. RESULTS: There was significant reduction of all variables in the intervention group (p&lt;0.01), but the control group had no change in waist circumference (p=0.06) and hip circumference (p=0.36). Patients of the intervention group presented greater weight loss (4.4kg, p&lt;0.01) and reduction of body mass index, waist circumference, waist circumference 1, waist circumference 2 (p&lt;0.01), and fat mass (p=0.04) than the control group. CONCLUSION: The weight loss and body composition changes were greater in the low-carbohydrate group than in the conventional-diet group, and they had a greater impact on waist measurements. The proposed measurements (waist circumference 1 and waist circumference 2) were sensitive to weight reduction in both groups.
DOI: 10.6084/m9.figshare.20018252
title: Factors associated with serum lipids of adolescents from the Brazilian South

description: OBJECTIVE: This study analyzes factors associated with serum lipids in adolescents from southern Brazil. METHODS: This is a school-based cross-sectional study with a probabilistic sample composed of 660 adolescents aged 14 to 19 years from the city of Três de Maio, Rio Grande do Sul state, Brazil. The following independent variables were analyzed: body mass index; waist-to-height ratio; moderate to vigorous level of physical activity; sedentary behaviour; lipid, total saturated fatty acid, cholesterol, sodium, and fiber intakes; smoking; and alcohol abuse. Linear regression analysis tested the association between the independent variables and total cholesterol and high density lipoprotein-cholesterol. RESULTS: Body mass index was directly associated with total cholesterol (β=0.96, p=0.001) and reversely associated with high density lipoprotein-cholesterol (β=-0.45, p&lt;0.001). CONCLUSION: High body mass index may have a negative impact on the lipid profile of adolescents. Thus, monitoring nutritional status is important to prevent and control dyslipidemia in adolescents. We suggest multidisciplinary and intersectoral actions that encourage teenagers to acquire a healthy lifestyle, with emphasis on the adoption of an active lifestyle and balanced diet.