cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11930190
title: Ruthenium(II)-mercapto Complexes with Anticancer Activity Interact with Topoisomerase IB

description: Herein we present four new ruthenium(II) complexes: [Ru(mtz)2(dppb)] (1), [Ru(mmi)2(dppb)] (2), [Ru(dmp)2(dppb)] (3), and [Ru(mpca)2(dppb)] (4), where mtz = 2-mercaptothiazoline; mmi = 2-mercapto-1-methyl-imidazole; dmp = 4,6-diamino-2-mercaptopyrimidine; mpca = 6-mercaptopyridine-3-carboxylic acid; dppb = 1,4-bis(diphenylphosphino)butane. In vitro cell culture experiments revealed cytotoxic activity for complexes 2, 3 and 4 against MCF-7 (breast, non-invasive), MDA-MB-231 (breast, invasive), A549 (lung), DU-145 (prostate) and HepG2 (liver) tumor cells, in some cases lower than the half maximal inhibitory concentration (IC50) for the reference drug (cisplatin). The deoxyribonucleic acid (DNA) interactions studied by viscosity measurements, gel electrophoresis and square-wave voltammetry indicated that the DNA binding affinity primarily occurs through non-covalent interactions. Only complex 2 was able to fully inhibit the DNA supercoiled relaxation mediated by human topoisomerase IB (Top IB). The analysis indicates that complex 2 inhibits the cleavage and the reconnection steps of the catalytic cycle, being both a poison and a catalytic inhibitor.
DOI: 10.6084/m9.figshare.11930193
title: Cycle pruning programmed on the grain yield of arabica coffee

description: ABSTRACT The cycle pruning programmed is a reinvigoration technique widely employed in Conilon coffee. This strategy may also be adopted for Arabica coffee to increase its crop longevity and yield. In this scenario, the present study proposes to examine the influence of the cycle pruning programmed on the vegetative and productive development of Arabica coffee. The experiment was developed in the field as a randomized-block design with four replicates. Treatments were tested in a 4 × 2 factorial arrangement consisting of four stem densities (4000, 8000, 12000 or 16000 stems ha-1) and two management strategies for the removal of plagiotropic branches (annual or biennial removal of branches which had reached 70% or more of their production capacity). For some variables, the treatments were organized as a 4 × 2 × 2 factorial arrangement in which the last factor corresponded to two regions of data collection in the plant (upper or lower). For all factorial arrangements, an additional treatment (control) was employed corresponding to traditional pruning (recepa). Annual or biennial removal of plagiotropic branches which had attained 70% or more of their production capacity can be adopted with no losses to crop yield. The cycle pruning programmed improves the distribution of branches, increases canopy area and prevents the occurrence of the “naked neck” phenomenon, observed in control treatment. Additionally, it provides better agronomic and productive performance; therefore, it can be used as a substitute for recepa.
DOI: 10.6084/m9.figshare.11930202.v1
title: Selective Entrapment of Pb2+ from Fresh Thunbergia laurifolia Leaves Extract and Thunbergia laurifolia Tea Extract

description: The leaves of Thunbergia laurifolia and its tea were extracted by water. The abilities to chelate heavy metal ions of the extracts were studied by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and atomic absorption spectroscopy (AAS). The results showed that the extracts exhibited high selectivity for Pb2+ chelation via a favourable-selective precipitation to Pb2+ in aqueous solutions compared to other metal ions, such as Zn2+, Cu2+ and Fe3+. The Pb2+ removal capability of the extracts were 51-52%. The selective Pb2+-trapping process of the extracts of Thunbergia laurifolia could be attributed to predominant presence of the phytochemical compounds, tannin and saponin, in the Thunbergia laurifolia leave and tea. Moreover, Thunbergia laurifolia tea also exhibited antioxidant property as demonstrating by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical method.
DOI: 10.6084/m9.figshare.11930190.v1
title: Ruthenium(II)-mercapto Complexes with Anticancer Activity Interact with Topoisomerase IB

description: Herein we present four new ruthenium(II) complexes: [Ru(mtz)2(dppb)] (1), [Ru(mmi)2(dppb)] (2), [Ru(dmp)2(dppb)] (3), and [Ru(mpca)2(dppb)] (4), where mtz = 2-mercaptothiazoline; mmi = 2-mercapto-1-methyl-imidazole; dmp = 4,6-diamino-2-mercaptopyrimidine; mpca = 6-mercaptopyridine-3-carboxylic acid; dppb = 1,4-bis(diphenylphosphino)butane. In vitro cell culture experiments revealed cytotoxic activity for complexes 2, 3 and 4 against MCF-7 (breast, non-invasive), MDA-MB-231 (breast, invasive), A549 (lung), DU-145 (prostate) and HepG2 (liver) tumor cells, in some cases lower than the half maximal inhibitory concentration (IC50) for the reference drug (cisplatin). The deoxyribonucleic acid (DNA) interactions studied by viscosity measurements, gel electrophoresis and square-wave voltammetry indicated that the DNA binding affinity primarily occurs through non-covalent interactions. Only complex 2 was able to fully inhibit the DNA supercoiled relaxation mediated by human topoisomerase IB (Top IB). The analysis indicates that complex 2 inhibits the cleavage and the reconnection steps of the catalytic cycle, being both a poison and a catalytic inhibitor.
DOI: 10.6084/m9.figshare.11930196.v1
title: Production engineers profiling: competences of the professional the market wants

description: Abstract Paper aims Identify the differences in the competences of the production engineering alumni and the importance attributed to it by the job market. Originality This is funded on investigating the gap between the competences the academy and the job market attribute to the professional of production engineering. Research method A questionnaire with production engineers was conducted to collect data that was analyzed using descriptive and predictive quantitative methods. Main findings Soft skills have higher importance to the job market but are the less addressed by universities. Professional competence importance is dependent on the sector. The skills with the higher gaps are associated with the constant transformation of industries and the increasing complexity of decision-making. Implications for theory and practice Theory should adopt a strategic orientation to guide the prioritization of competences and practice must adapt selection mechanisms to test for soft skills and customize it to the professional sector.
DOI: 10.6084/m9.figshare.11930184
title: Carcinoid of the gallbladder in two dogs

description: ABSTRACT: A carcinoid is a rare neoplasm derived from neuroendocrine cells that may arise in the hepatobiliary system. This report described two cases of carcinoid of the gallbladder in dogs, with emphasis on the clinical, pathological and immunohistochemical aspects. Case 1 was a 10-year-old female Shih Tzu, with a history of prostration and occasional episodes of diarrhea and vomiting. Case 2 was a 10-year-old male Cocker Spaniel, with a history of diarrhea and abdominal pain. Abdominal ultrasonography suggested a neoplasm of the gallbladder in both cases. The dogs underwent cholecystectomy, and 10% formalin-fixed samples were sent for pathological examination. Histologically, a neoplastic proliferation of neuroendocrine cells was observed in the gallbladder submucosa of both cases. Immunohistochemical labeling for neuroendocrine antibodies, such as synaptophysin, neuron-specific enolase and chromogranin A, confirmed the diagnoses. Carcinoid of the gallbladder is a rare neoplasm in dogs, without specific clinical signs, but should be included in the differential diagnosis of lesions that affect this organ.
DOI: 10.6084/m9.figshare.11930187
title: Tribute to Professor Andrew J. Lees

description: Abstract Professor Andrew John Lees, from the National Hospital for Neurology and Neurosurgery, a neurological hospital in Queen Square, London, UK, has contributed in a stupendous way to the development of the field of movement disorders in Brazil, with a constant and intense participation in numerous congresses and scientific meetings of this specialty since 1983.
DOI: 10.6084/m9.figshare.11930193.v1
title: Cycle pruning programmed on the grain yield of arabica coffee

description: ABSTRACT The cycle pruning programmed is a reinvigoration technique widely employed in Conilon coffee. This strategy may also be adopted for Arabica coffee to increase its crop longevity and yield. In this scenario, the present study proposes to examine the influence of the cycle pruning programmed on the vegetative and productive development of Arabica coffee. The experiment was developed in the field as a randomized-block design with four replicates. Treatments were tested in a 4 × 2 factorial arrangement consisting of four stem densities (4000, 8000, 12000 or 16000 stems ha-1) and two management strategies for the removal of plagiotropic branches (annual or biennial removal of branches which had reached 70% or more of their production capacity). For some variables, the treatments were organized as a 4 × 2 × 2 factorial arrangement in which the last factor corresponded to two regions of data collection in the plant (upper or lower). For all factorial arrangements, an additional treatment (control) was employed corresponding to traditional pruning (recepa). Annual or biennial removal of plagiotropic branches which had attained 70% or more of their production capacity can be adopted with no losses to crop yield. The cycle pruning programmed improves the distribution of branches, increases canopy area and prevents the occurrence of the “naked neck” phenomenon, observed in control treatment. Additionally, it provides better agronomic and productive performance; therefore, it can be used as a substitute for recepa.
DOI: 10.6084/m9.figshare.11930181
title: SCHOOLS IN MAPUCHE TERRITORY: INEQUALITIES IN THE CHILEAN CONTEXT

description: ABSTRACT The article presents an exploratory study on results in the evaluation tests of the Sistema de Medición de la Calidad de la Educación (Education Quality Measurement System), in reading comprehension, from 2011 to 2015, in La Araucanía, Chile. Methodologically, the results obtained by a sample of 22 schools located in Mapuche communities are described. The results show that rural municipal schools obtain better results compared to subsidized private schools with a difference of 8 to 10 points. This is relevant, because in Mapuche communities there is a supremacy of subsidized private schools, which systematically obtain low scores, which would continue perpetuating social and educational inequalities in the territory between Mapuche and non-Mapuche.
DOI: 10.6084/m9.figshare.11930184.v1
title: Carcinoid of the gallbladder in two dogs

description: ABSTRACT: A carcinoid is a rare neoplasm derived from neuroendocrine cells that may arise in the hepatobiliary system. This report described two cases of carcinoid of the gallbladder in dogs, with emphasis on the clinical, pathological and immunohistochemical aspects. Case 1 was a 10-year-old female Shih Tzu, with a history of prostration and occasional episodes of diarrhea and vomiting. Case 2 was a 10-year-old male Cocker Spaniel, with a history of diarrhea and abdominal pain. Abdominal ultrasonography suggested a neoplasm of the gallbladder in both cases. The dogs underwent cholecystectomy, and 10% formalin-fixed samples were sent for pathological examination. Histologically, a neoplastic proliferation of neuroendocrine cells was observed in the gallbladder submucosa of both cases. Immunohistochemical labeling for neuroendocrine antibodies, such as synaptophysin, neuron-specific enolase and chromogranin A, confirmed the diagnoses. Carcinoid of the gallbladder is a rare neoplasm in dogs, without specific clinical signs, but should be included in the differential diagnosis of lesions that affect this organ.
DOI: 10.6084/m9.figshare.11930178
title: Development of mortar for laying and coating with pineapple fibers

description: ABSTRACT Brazil is one of the largest producers of pineapple in the world, which, in addition to bringing substantial benefits, contributes to increasing waste generation in the stages of processing, aiming at its consumption. The Brazilian environmental legislation has been increasingly more restrictive with respect to the disposal of solid waste, whether domestic, industrial or agro-industrial, which has stimulated studies related to the use of these materials in several matrices. This study aimed to evaluate the potentiality of using the natural fiber of pineapple (Ananas comosus), extracted from its leaves, as reinforcing material in cementitious composites. For this purpose, natural fiber extracted from the pineapple crown was incorporated in different percentages in relation to the cement mass: 0, 2.5, 5, 7.5 and 10%, and the fibers obtained were submerged in aqueous NaOH solution with 5% concentration in volume, for 1 h at room temperature. The following variables were evaluated: consistency, water retention, incorporated air, mass density in hardened state, mechanical strength, water absorption by immersion and capillarity, besides verifying the influence of the mode of fiber incorporation. It was observed that the treatment with NaOH solution and the percentage of natural pineapple fiber incorporated in up to 5% in relation to the cement mass form mortars with technological feasibility.
DOI: 10.6084/m9.figshare.11930181.v1
title: SCHOOLS IN MAPUCHE TERRITORY: INEQUALITIES IN THE CHILEAN CONTEXT

description: ABSTRACT The article presents an exploratory study on results in the evaluation tests of the Sistema de Medición de la Calidad de la Educación (Education Quality Measurement System), in reading comprehension, from 2011 to 2015, in La Araucanía, Chile. Methodologically, the results obtained by a sample of 22 schools located in Mapuche communities are described. The results show that rural municipal schools obtain better results compared to subsidized private schools with a difference of 8 to 10 points. This is relevant, because in Mapuche communities there is a supremacy of subsidized private schools, which systematically obtain low scores, which would continue perpetuating social and educational inequalities in the territory between Mapuche and non-Mapuche.
DOI: 10.6084/m9.figshare.11930187.v1
title: Tribute to Professor Andrew J. Lees

description: Abstract Professor Andrew John Lees, from the National Hospital for Neurology and Neurosurgery, a neurological hospital in Queen Square, London, UK, has contributed in a stupendous way to the development of the field of movement disorders in Brazil, with a constant and intense participation in numerous congresses and scientific meetings of this specialty since 1983.
DOI: 10.6084/m9.figshare.11930175
title: Instant coffee with steamed PVA beans: Physical-chemical and sensory aspects

description: ABSTRACT Around 20% of Brazilian coffee production corresponds to defective beans (PVA), which decreases the quality of the coffee brews. Steam treatment has been reported as an alternative to improve the cup quality of coffee products. This research aimed to study the feasibility of using steamed Robusta PVA beans in instant coffee products. After the steam treatment (2 bar for 3 min), the Robusta PVA was dried, roasted, subjected to extraction in a pilot plant, and freeze-dried. The steamed and untreated Robusta PVA materials (raw, roasted, and instant coffees) and blends of instant Arabica coffee with PVA were characterized. The steamed Robusta PVA instant coffee presented 7.01 g caffeine and 8.74 g total chlorogenic acids in 100 g product. Instant coffee blends with 30% and 50% of steamed Robusta PVA in Arabica coffee were studied. A sensory difference between blends with steamed and untreated Robusta PVA was only perceived by the addition of 50% PVA. The blend with 50% of steamed coffee was preferred and well accepted (average grade of 7.9 on a 10-scale). The use of steamed Robusta PVA coffee (under mild steam treatment conditions, 2 bar/3 min) in instant coffee was viable, and it was obtained an instant coffee to be used in a blend with 50% of Arabica coffee with a good profile of bioactive compounds and sensory acceptance.
DOI: 10.6084/m9.figshare.11930175.v1
title: Instant coffee with steamed PVA beans: Physical-chemical and sensory aspects

description: ABSTRACT Around 20% of Brazilian coffee production corresponds to defective beans (PVA), which decreases the quality of the coffee brews. Steam treatment has been reported as an alternative to improve the cup quality of coffee products. This research aimed to study the feasibility of using steamed Robusta PVA beans in instant coffee products. After the steam treatment (2 bar for 3 min), the Robusta PVA was dried, roasted, subjected to extraction in a pilot plant, and freeze-dried. The steamed and untreated Robusta PVA materials (raw, roasted, and instant coffees) and blends of instant Arabica coffee with PVA were characterized. The steamed Robusta PVA instant coffee presented 7.01 g caffeine and 8.74 g total chlorogenic acids in 100 g product. Instant coffee blends with 30% and 50% of steamed Robusta PVA in Arabica coffee were studied. A sensory difference between blends with steamed and untreated Robusta PVA was only perceived by the addition of 50% PVA. The blend with 50% of steamed coffee was preferred and well accepted (average grade of 7.9 on a 10-scale). The use of steamed Robusta PVA coffee (under mild steam treatment conditions, 2 bar/3 min) in instant coffee was viable, and it was obtained an instant coffee to be used in a blend with 50% of Arabica coffee with a good profile of bioactive compounds and sensory acceptance.
DOI: 10.6084/m9.figshare.11930178.v1
title: Development of mortar for laying and coating with pineapple fibers

description: ABSTRACT Brazil is one of the largest producers of pineapple in the world, which, in addition to bringing substantial benefits, contributes to increasing waste generation in the stages of processing, aiming at its consumption. The Brazilian environmental legislation has been increasingly more restrictive with respect to the disposal of solid waste, whether domestic, industrial or agro-industrial, which has stimulated studies related to the use of these materials in several matrices. This study aimed to evaluate the potentiality of using the natural fiber of pineapple (Ananas comosus), extracted from its leaves, as reinforcing material in cementitious composites. For this purpose, natural fiber extracted from the pineapple crown was incorporated in different percentages in relation to the cement mass: 0, 2.5, 5, 7.5 and 10%, and the fibers obtained were submerged in aqueous NaOH solution with 5% concentration in volume, for 1 h at room temperature. The following variables were evaluated: consistency, water retention, incorporated air, mass density in hardened state, mechanical strength, water absorption by immersion and capillarity, besides verifying the influence of the mode of fiber incorporation. It was observed that the treatment with NaOH solution and the percentage of natural pineapple fiber incorporated in up to 5% in relation to the cement mass form mortars with technological feasibility.
DOI: 10.6084/m9.figshare.11930172
title: Foliar fertilization on the production of grafted dwarf cashew seedlings

description: ABSTRACT Foliar fertilization is an important agricultural technique that has stood out for its ease of use and can contribute to the production of high-quality and -vigor cashew seedlings. The objective of this study was to evaluate the effect of foliar fertilizer rates and time of application on ‘BRS 226’ dwarf cashew seedlings after grafting. The experiment was carried out in a completely randomized design, with five treatments and 24 replicates. Each experimental unit consisted of one plant. The commercial product (NPK 20-20-20) was used at the recommended rate for fruit crops in general, 2.5 g L-1. Foliar fertilizer applications at 45 and 60 days after grafting (DAG), with 50% of the recommended rate (1.25 g L-1), or at 80 DAG, with 100% of the recommended, promoted increments of 9% and 13% in the total dry mass, respectively, in comparison to unfertilized plants. Besides that, at 80 DAG the foliar fertilizer application caused significant difference in the B contents, allowed obtaining better quality ‘BRS 226’ grafted dwarf-cashew seedlings. In the production of grafted seedlings of ‘BRS 226’ dwarf-cashew, it’s recommended to foliar fertilization at 45 and 60 days after grafting, with 1.25 g L-1 of the product based on NPK 20-20-20; or a single dose application 2.5 g L-1 at 80 days after grafting.
DOI: 10.6084/m9.figshare.11930169
title: Surface roughness of edge glued panels (EGP) of amazon maneged species

description: ABSTRACT In the Amazon, the sustainable use of wood is associated with technological research that indicates industrial use. Surface roughness of wood is an important criterion for assessing tool condition, machining performance and product quality. The objective of this study was to evaluate the surface roughness of five Amazonian woods used in the manufacture of EGP (Edge Glued Panels).The woods Angelim pedra, Angelim vermelho, Breu vermelho, Murici and Piãozinho, obtained from managed areas of the Amazon, were used in the manufacture of EGP panels. Surface treatments were performed with 120 and 180 grit sandpaper. Surface ripples (roughness) were measured using a digital roughness meter with needle (n = 180 readings). Physical and mechanical tests were performed on both wood and EGP samples, to verify the possible relationship between these properties and roughness. EGP Piãozinho presented better surface quality, which consists of lower roughness (lower surface wave height), while EGP Angelim pedra presented uneven surfaces. Surface quality was satisfactory when 120 grain sandpaper was applied to most products. The results showed a highly significant effect of roughness such as the physical-mechanical properties. The highlight of the study is the wood and EGP Piãozinho, because in all the tests, it always presented the best performances.
DOI: 10.6084/m9.figshare.11930163
title: Anticancer drug screening: standardization of in vitro wound healing assay

description: ABSTRACT Introduction: Gliomas are characterized by rapid proliferation and aggressive invasion into normal surrounding brain tissue. In medical laboratories, the in vitro wound healing assay stands out as a simple, easy, inexpensive and affordable method to evaluate cell migration and proliferation. Objective: To standardize the in vitro wound healing assay using antimicrotubule drugs as positive controls. Methods: U87MG glioma cells were seeded at different densities and, after 24 h, the monolayer was scratched using different micropipette tip size to create a gap with no cells. The cells were then treated with colchicine and paclitaxel in culture medium with the presence or absence of fetal bovine serum. The wound was photographed with the aid of an inverted microscope and the wound area was measured using the Image J software. Results: Better defined edges scratches and monolayer with approximately 90% confluence were obtained at 1.5 and 2 x 105 cells/well density. The width and area of the scratch were, respectively, 948 pm/2.193221 mm2; 964 pm/2.266 mm2 and 1448 pm/3.221 mm2 to 10. 200 and 1000 pl micropipette tips. Colchicine inhibited wound closure by 12.6% or 3.4%, both in the presence or absence of serum; paclitaxel 2.4 and 6.7% respectively. Conclusion: Under standardized conditions, colchicine and paclitaxel proved to be efficient positive controls into the in vitro wound healing assay.