DOI: 10.5061/dryad.7sq5c
title: Data from: Systems and trans-system level analysis identifies conserved iron deficiency responses in the plant lineage

description: We surveyed the iron nutrition-responsive transcriptome of Chlamydomonas
 reinhardtii using RNA-Seq methodology. Presumed primary targets were
 identified in comparisons between visually asymptomatic iron-deficient
 versus iron-replete cells. This includes the known components of
 high-affinity iron uptake as well as candidates for distributive iron
 transport in C. reinhardtii. Comparison of growth-inhibited iron-limited
 versus iron-replete cells revealed changes in the expression of genes in
 chloroplastic oxidative stress response pathways, among hundreds of other
 genes. The output from the transcriptome was validated at multiple levels:
 by quantitative RT-PCR for assessing the data analysis pipeline, by
 quantitative proteomics for assessing the impact of changes in RNA
 abundance on the proteome, and by cross-species comparison for identifying
 conserved or universal response pathways. In addition, we assessed the
 functional importance of three target genes, VITAMIN C 2 (VTC2),
 MONODEHYDROASCORBATE REDUCTASE 1 (MDAR1), and CONSERVED IN THE GREEN
 LINEAGE AND DIATOMS 27 (CGLD27), by biochemistry or reverse genetics. VTC2
 and MDAR1, which are key enzymes in de novo ascorbate synthesis and
 ascorbate recycling, respectively, are likely responsible for the 10-fold
 increase in ascorbate content of iron-limited cells. CGLD27/At5g67370 is a
 highly conserved, presumed chloroplast-localized pioneer protein and is
 important for growth of Arabidopsis thaliana in low iron.
description: Supplemental Data Set
 1Summary of RNA-Seq
 statistics (sheet1) and transcript abundance for all Augustus 10.2 C.
 reinhardtii genes (sheet 2).Supplemental Data Set
 2Differentially
 accumulating RNAs in photoheterotrophic conditions (3
 sheets).Supplemental Data Set
 3Differentially
 accumulating RNAs in photoautotrophic conditions (3
 sheets).Supplemental Data Set
 4Differentially
 accumulating RNAs in photoautotrophic versus photoheterotrophic conditions
 (10 sheets).Supplemental Data Set
 5Comparison of mRNA
 abundances for genes encoding metal transporters.Supplemental Data Set
 6Comparison of mRNA
 abundances for genes encoding enzymes of the isoprenoid and carotenoids
 biosynthesis/degradation pathways.Supplemental Data Set
 7Summary of protein
 abundances from MSE analysis (2 sheets).Supplemental Data Set
 8Comparison of mRNA
 abundances for genes encoding carbon metabolism
 proteins.Supplemental Data Set
 9Comparison of mRNA
 abundances for genes encoding subunits of the tetrapyrrole biosynthesis
 and degradation pathways.Supplemental Data Set
 10Amino acid sequence
 alignment used to produce Figure 5B.Supplemental Data Set
 11Amino acid sequence
 alignment used to produce Supplemental Figure 5B.Supplemental Data Set
 12Amino acid sequence
 alignment used to produce Supplemental Figure 6B.Supplemental Data Set
 13Amino acid sequence
 alignment used to produce Supplemental Figure 7B.Supplemental Data Set
 14Amino acid sequence
 alignment used to produce Supplemental Figure 8B.Supplemental Data Set
 15Amino acid sequence
 alignment used to produce Supplemental Figure 10B.Supplemental Data Set
 16Amino acid sequence
 alignment used to produce Supplemental Figure 13B.