DOI: 10.5061/dryad.8s449qb
title: Data from: Signatures of local adaptation along environmental gradients in a range-expanding damselfly (Ischnura elegans)

description: Insect distributions are shifting rapidly in response to climate change
 and are undergoing rapid evolutionary change. We investigate the molecular
 signatures underlying local adaptation in the range-expanding damselfly,
 Ischnura elegans. Using a landscape genomic approach combined with
 generalized dissimilarity modelling (GDM), we detect selection signatures
 on loci via allelic frequency change along environmental gradients. We
 analyse 13,612 Single Nucleotide Polymorphisms (SNPs), derived from
 Restriction site-Associated DNA sequencing (RADseq), in 426 individuals
 from 25 sites spanning the I. elegans distribution in Sweden, including
 its expanding northern range edge. Environmental association analysis
 (EAA) and the magnitude of allele frequency change along the range
 expansion gradient revealed significant signatures of selection in
 relation to high maximum summer temperature, high mean annual
 precipitation, and low wind speeds at the range edge. SNP annotations with
 significant signatures of selection revealed gene functions associated
 with ongoing range expansion, including heat shock proteins (HSP40 and
 HSP70), ion transport (V-ATPase) and visual processes (long
 wavelength-sensitive opsin), which have implications for thermal stress
 response, salinity tolerance and mate discrimination, respectively. We
 also identified environmental thresholds where climate-mediated selection
 is likely to be strong, and indicate that I. elegans is rapidly adapting
 to the climatic environment during its ongoing range expansion. Our
 findings empirically validate an integrative approach for detecting
 spatially explicit signatures of local adaptation along environmental
 gradients.
description: Ischnura elegans draft
 genome assembly Dec 2015This is a draft assembly
 of the Ischnura elegans genome produced by Pallavi Chauhan, Bengt Hansson,
 et al. (unpublished). Production date was December, 2015. An updated and
 annotated reference genome for Ischnura elegans is scheduled to be
 available on NCBI in 2018.Ischnura_elegans_draftgenome_assemblyDec2015.fastaCompleteness Report for I.elegans draft genome Dec 15Completeness Report for I.elegans draft genome, Dec 2015Ischnura_elegans_draftgenomeDec2015.output.completeness_reportReport for Ischnura_elegans_draftgenomeDec2015Assembly summary data, coverage and statistics for I.elegans draft genome December 2015Ischnura_elegans_draftgenomeDec2015.reportGDM R code for single SNP-based analysisGDM R code for single SNP-based analysis - data formatting, modelling and outputGDM Upload 20180412.REnvironmental dataEnvironmental data for variables used in GDM and EAAUpload1_EnvData.csvSNP_datafileDatafile for 13612 SNPs in genepop format, generated from Stacks 1.40.PlinkMap_datafilePlink .map datafile for 13612 SNPsGDM_TotalmodelOutput_1758snpsTotal model output for GDM of 1758 candidate SNPsSNPannotations and GDMSNP annotations for all candidate loci and GDM output for SNPs meeting criteria for presentation.SNPannotations.xlsxpopmapPopmap of population site IDs and sample IDsOutlier_EAA_SNPs1758List of SNP IDs for all uniquely identified SNPs from Fst Outlier (Bayescan and OutFlank) and Environmental Association Analysis (LFMM) tests.