cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/nvv6b-av859
title: H.pylori 26695 delta-cagH

description: Grid box: YWC090.As controls for the absence of the membrane-bound tubes when cultured without AGS cells.
description: <strong>Tilt Series Date:</strong> 2014-03-02</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-02-2_slicer9881.jpg: Helicobacter pylori .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-2/rawdata/</p>20140302_YWC090_10002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-2/rawdata/20140302_YWC090_10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140302_YWC090_10002_part131_10.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-02-2/3dimage_39464/20140302_YWC090_10002_part131_10.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/746qb-es040
title: H. pylori 26695 / AGS

description: Grid box YWC054
description: <strong>Tilt Series Date:</strong> 2013-11-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-14/rawdata/</p>20131125_YWC054_10014.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-14/rawdata/20131125_YWC054_10014.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131125_YWC054_10014_part121_4.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-14/3dimage_35043/20131125_YWC054_10014_part121_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mvz5q-w1t48
title: Sulfolobus cells synchronized fo

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2014-02-04</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's samples gridbox 8.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 5 sec, sponge for humidity</p>
  rra2014-02-04-43_slicer8820.jpg: pili .  Sulfolobus acidocaldarius rra2014-02-04-43_slicer8821.jpg: filament .  Sulfolobus acidocaldarius rra2014-02-04-43_slicer8822.jpg: filament .  Sulfolobus acidocaldarius rra2014-02-04-43_slicer8825.jpg: flagella? .  Sulfolobus acidocaldarius
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-43/rawdata/</p>atlas60006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-43/rawdata/atlas60006.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas60006_part111_6.rec,  Reconstruction (Pixel Size 1.56 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-02-04-43/3dimage_38410/atlas60006_part111_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jz9p3-rqs02
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-25-50_slicer10132.jpg: helicobacter pylori .  ribosome exclusion zone .  chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-50/rawdata/</p>20131025_Janelia_YWC043_10004.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-50/rawdata/20131025_Janelia_YWC043_10004.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_10004_part131_6.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-50/3dimage_33497/20131025_Janelia_YWC043_10004_part131_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fpa42-05s90
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter1 558 lysed/PVP
description: <strong>Tilt Series Date:</strong> 2013-10-04</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> -     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xgBuffer 1:10% Polyvinylpyrrolidone (PVP)1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-04-24/rawdata/</p>Rhodopvp0024.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-04-24/rawdata/Rhodopvp0024.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp0024SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-04-24/3dimage_46397/Rhodopvp0024SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6mq4s-vd027
title: Hippocampal Neurons (cultured)

description: These tomograms were shot at -4 um defocus with total dose of 80 electrons.
description: <strong>Tilt Series Date:</strong> 2014-12-07</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -4.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
  mst2014-12-07-4_slicer12690.jpg: Long actin filaments running along long axis of axon. mst2014-12-07-4_slicer12691.jpg: 13 microtubles
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-07-4/rawdata/</p>120714_hipp_neuron_tomo0004.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-07-4/rawdata/120714_hipp_neuron_tomo0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        120714_hipp_neuron_tomo0004_full.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-12-07-4/3dimage_48620/120714_hipp_neuron_tomo0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cpdww-8hj09
title: Myxococcus xanthus PilP-sfGFP

description: Strain SA4070Grid box YWC093
description: <strong>Tilt Series Date:</strong> 2014-03-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-08-11_slicer3973.jpg: Myxococcus xanthus PilP-sfGFP.  .  T4Ps. .  Membrane-bound plate with bulging outter membrane. ycw2014-03-08-11_slicer7169.jpg: Type VI Secretion system? ycw2014-03-08-11_slicer7171.jpg: filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-11/rawdata/</p>20140308_YWC093_10037.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-11/rawdata/20140308_YWC093_10037.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140308_YWC093_10037_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-08-11/3dimage_40022/20140308_YWC093_10037_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a6nek-p9b56
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2013-12-21-35_slicer8569.jpg: Type 6 Secretion System?  T6SS? Myxococcus Xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-35/rawdata/</p>20131221_YWC063_10035.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-35/rawdata/20131221_YWC063_10035.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_10035_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-35/3dimage_36408/20131221_YWC063_10035_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ec3ym-15k07
title: S. pombe (Cdc25-22)

description: Cryosections (180 nm nominal thickness)
description: <strong>Tilt Series Date:</strong> 2014-04-19</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mithilesh Mishra, Mohan Balasubramanian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Synchronized cytokinesis. From OD 600 = 0.25 grow for 4 hrs at 36 C. Return to 25 C for 45 min then begin monitoring ring formation by LM. Once 80-90 of rings are continuous they were mixed with Dextran (100 ul of 40% Dextran in ~ 400 ul of pelleted cells). They were then high pressure frozen.</p>
<strong>Sample Preparation:</strong> High pressure freezing followed by cryosectioning</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-15/3dimage_42048/</p>041914_pombe_0015.rec,  Reconstruction (Pixel Size 2.4 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-15/3dimage_42048/041914_pombe_0015.rec"
        > <i class="download icon"></i> Download </a></p>

        041914_pombe_0015.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-19-15/rawdata/041914_pombe_0015.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/epx7m-hzt07
title: Bdellovibrio attack-phase

description: In the mixture with WM3433 E. coli mini cells.Something wrong with the dosage setting or shutter.
description: <strong>Tilt Series Date:</strong> 2013-09-10</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2.0°, constant angular increment, dosage: 20.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett, Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
  ycw2013-09-10-36_slicer10348.jpg: chemoreceptor, Bdellovibrio bacteriovorus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-10-36/rawdata/</p>20130910_Janelia_Bdellovibrio_WM3433_20007.mrc,  Tilt Series (Pixel Size 0.42 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-10-36/rawdata/20130910_Janelia_Bdellovibrio_WM3433_20007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130910_Janelia_Bdellovibrio_WM3433_20007_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-10-36/3dimage_30795/20130910_Janelia_Bdellovibrio_WM3433_20007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z7prv-yqj90
title: E. coli CusCFBA-CusC-K98H

description: Overexpressed CusCFBA efflux pump in E. coli C43 (DE3).Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-06-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Edward Yu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.</p>
  ycw2013-06-07-18_slicer8021.jpg: CusCFBA .  Escherichia coli ycw2013-06-07-18_slicer8023.jpg: CusCFBA .  Escherichia coli ycw2013-06-07-18_slicer8026.jpg: CusCFBA ycw2013-06-07-18_slicer8031.jpg: CusCFBA .  Escherichia coli
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-07-18/rawdata/</p>CusCFBA_CusCK98H_20015.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
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        CusCFBA_CusCK98H_20015_part121_10.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
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