DOI: 10.5061/dryad.69p8cz900
title: Plant DNA metabarcoding records from three cores of lacustrine lakes along a open larch forest-forest tundra-tundra transect at the Omoloy region, north-eastern Siberia

description: Here, we provide the raw plant DNA metabarcoding data archived in three
 Siberian lake sediment cores spanning the mid-Holocene to the present
 (7.6-0 cal ka BP), from northern typical tundra to southern open larch
 forest in the Omoloy region. There are three cores: 14-OM-20B, Lat. / °:
 70.53, Lon. / °: 132.91, Ele. / m a.s.l.: 52, Modern vegetation: open
 larch forest, Lake area / km2: 0.26, Maximal depth / m: 3.4 14-OM-02B,
 Lat. / °: 70.72, Lon. / °: 132.67, Ele. / m a.s.l.: 58, Modern vegetation:
 forest tundra, Lake area / km2: 0.08, Maximal depth / m: 3.5 14-OM-12A,
 Lat. / °: 70.96, Lon. / °: 132.57, Ele. / m a.s.l.: 60, Modern vegetation:
 tundra, Lake area / km2: 0.09, Maximal depth / m: 4.5 Three lake sediment
 cores, 14OM12A (33 cm long), 14OM02B (49.5 cm long) and 14OM20B (86 cm
 long), were recovered from three sites using a UWITEC gravity corer (6 cm
 internal diameter) equipped with a hammer tool in July 2014. From the
 three cores, 16 bulk organic carbon samples were selected because of the
 lack of macrofossil remains and radiocarbon dated using accelerator mass
 spectrometry (AMS) at Poznań radiocarbon laboratory of Adam Mickiewicz
 University, Poland. In addition, 30 freeze-dried samples per core at 0.25
 or 0.5 cm intervals between 0 and 15 cm were analysed for 210Pb/137Cs at
 the Liverpool University Environmental Radioactivity Laboratory. (The
 dating table has been submitted to PANGAEA) In this project, we analyse
 pollen (has been submitted to PANGAEA, doi: 10.1594/PANGAEA.922550) and
 sedaDNA from three lake sediment cores from the Omoloy region in
 north-eastern Siberia (northern Yakutia), which are currently surrounded
 by different vegetation types ranging from typical tundra to open larch
 forest. First, our aim is to compare sedaDNA with the pollen data to see
 whether both methods track the same pattern with respect to compositional
 changes and diversity changes across the northern Russian treeline zone or
 are complementary to each other. Second, we reconstruct the mid- to
 late-Holocene changes of vegetation composition along a north–south
 transect. Third, we use the sedaDNA data to reconstruct variations in
 species richness and relate this to vegetation and climate change.
description: Plant DNA was extracted from about 2–4 g of sediment using the
 PowerMax<sup>®</sup> Soil DNA Isolation Kit (MoBio
 Laboratories, Inc., USA) in a dedicated palaeogenetic laboratory in AWI.
 The pooled PCR products were sequenced on the Illumina HiSeq platform (2 x
 125 bp, paired-end reads) at <i>Fasteris</i> SA sequencing
 service, Switzerland. To align, assign and filter Illumina sequences we
 used OBITools (Boyer et al., 2016).  The raw sequecning
 data (fastq files), scripts for creating the taxonomic database and
 analyzing the raw sequencing data, tag-sample-matirx, taxonomic database
 are enclosed.  
description: There is no any missing value. Scientists who are working on
 palaeoecology using environmental DNA from lake sediment core may be
 interested in this data.