DOI: 10.5061/dryad.547d7wm6q
title: Regional wetland plant responses to sulfur and other porewater chemistry in calcareous rich fens

description: Many wetland environmental gradients structure plant community
 composition, yet controls of plant community composition within rich fens,
 botanically diverse groundwater-fed wetlands, are still incompletely
 understood. Porewater chemistry and plant community composition were
 recorded for eight calcareous rich fens encompassing both calcium
 carbonate and calcium sulfate geological inputs in the Central New York
 State region. As expected, porewater sulfate and sulfide concentrations
 were on average higher for wetlands overlying calcium sulfate than for
 wetlands overlying calcium carbonate. However, within-wetland
 heterogeneity in porewater chemistry was high. Moss species density, moss
 cover, and total plant cover decreased with increased sulfide. Moss,
 dicot, and total cover also had a negative relationship with calcium.
 There were a number of species-level responses to calcium, sulfide,
 phosphorus, and ferrous iron. Plant height had a positive relationship
 with nitrogen. The strength and relative importance of some plant
 responses to sulfide and calcium in the current regional study differed
 from a previous sub-hectare scale study. The observed decrease in some
 metrics of site-level plant diversity with increased sulfide variability
 across fens highlighted the need to characterize species-environment
 relationships across spatial scales.
description: Sampling took place
 within eight calcareous rich fens (Fig. 1) in central New York State, USA.
 We established 10 sampling locations at each fen. In summer 2009 at
 each of the 10 sampling locations within each of the eight sites we
 measured plant community composition, porewater chemistry, and depth of
 peat. We recorded percent cover of each vascular and bryophyte species,
 bare ground, litter, and open water (if applicable), and the height of the
 overall tallest species in a 0.1 m x 0.1 m quadrat from 24 August - 9
 September 2009, with the exception of the Junius site that was sampled 6 -
 14 June 2009. In the center of each vegetation quadrat we installed a
 porewater sipper (outer diameter 1 cm) with a sample screen extending from
 8 to 12 cm below the soil surface, for an average sampling depth of 10 cm.
 We used a bulk interstitial porewater sipper because we sought to measure
 sulfide at a scale matching a large fraction of a plant’s
 rhizosphere. We collected
 porewater from each sipper using a hand syringe equipped with an in-line
 0.45 uM filter 13 - 16 September 2009, with the exception of the Junius
 site that was sampled 26 May - 1 June 2009. In the field, we placed
 subsamples in three 23 mL borosilicate glass scintillation vials. In one
 vial we immediately mixed a 11.5 mL sample with 11.5 mL of
 previously-added sulfide anti-oxidant buffer (SAOB), filling the vial to
 capacity to minimize headspace, and set this sample aside for sulfide
 analysis by ion selective electrode (ISE) in the lab as soon as possible.
 The SAOB was composed of NaOH, EDTA, and ascorbic acid to stabilize
 sulfide as S<sup>2-</sup> to avoid oxidation to sulfate or
 volatile loss as H<sub>2</sub>S. In a second vial we mixed 15
 mL of sample with 5 mL of Ferrozine and HEPES reagents for ferrous iron
 analysis in the lab. In a third vial we collected sample for lab analysis
 of calcium, sulfate, and Total Dissolved Nitrogen (TDN). An additional
 sample was used to measure field pH, conductivity, and temperature with a
 portable multimeter and then discarded. Finally, we deployed a pair of 2.5
 cm by 5 cm anion resin strips at each sampling location from 30 July - 14
 August 2009 at a depth of 1-3 cm and retrieved the strips 31 August - 16
 September 2009 for an index of phosphorus availability. As with the other
 measurements, the Junius resin deployment was earlier, from 10 - 11 May
 2009 to 8 - 12 June 2009. We measured
 porewater sulfide as S<sup>2-</sup> with an ion selective
 electrode (ISE) using sodium sulfide standards. We measured porewater
 Fe(II) (ferrous iron) spectrophotometrically at an absorbance of 562 nm.
 Aqueous porewater samples were submitted for inductively coupled plasma
 atomic-emission spectrometer (ICP-AES) analysis of calcium, ion
 chromatographic (IC) analysis of sulfate, and continuous-flow analysis
 (CFA) of Total Dissolved Nitrogen (TDN). Resin strips were extracted with
 0.5 M HCl and then the resin extract samples were reacted with
 Murphy-Riley reagents (sulfuric acid, ammonium molybdate, antimony
 potassium tartrate) and analyzed spectrophotometrically for phosphate at
 880 nm.
description: Metadata with units and a description for each of the variables
 in the dataset can be found in the ReadMe.csv file.