cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8848088
title: Changes in cardiac aldosterone and its synthase in rats with chronic heart failure: an intervention study of long-term treatment with recombinant human brain natriuretic peptide

description: The physiological mechanisms involved in isoproterenol (ISO)-induced chronic heart failure (CHF) are not fully understood. In this study, we investigated local changes in cardiac aldosterone and its synthase in rats with ISO-induced CHF, and evaluated the effects of treatment with recombinant human brain natriuretic peptide (rhBNP). Sprague-Dawley rats were divided into 4 different groups. Fifty rats received subcutaneous ISO injections to induce CHF and the control group (n=10) received equal volumes of saline. After establishing the rat model, 9 CHF rats received no further treatment, rats in the low-dose group (n=8) received 22.5 μg/kg rhBNP and those in the high-dose group (n=8) received 45 μg/kg rhBNP daily for 1 month. Cardiac function was assessed by echocardiographic and hemodynamic analysis. Collagen volume fraction (CVF) was determined. Plasma and myocardial aldosterone concentrations were determined using radioimmunoassay. Myocardial aldosterone synthase (CYP11B2) was detected by quantitative real-time PCR. Cardiac function was significantly lower in the CHF group than in the control group (P&lt;0.01), whereas CVF, plasma and myocardial aldosterone, and CYP11B2 transcription were significantly higher than in the control group (P&lt;0.05). Low and high doses of rhBNP significantly improved hemodynamics (P&lt;0.01) and cardiac function (P&lt;0.05) and reduced CVF, plasma and myocardial aldosterone, and CYP11B2 transcription (P&lt;0.05). There were no significant differences between the rhBNP dose groups (P&gt;0.05). Elevated cardiac aldosterone and upregulation of aldosterone synthase expression were detected in rats with ISO-induced CHF. Administration of rhBNP improved hemodynamics and ventricular remodeling and reduced myocardial fibrosis, possibly by downregulating CYP11B2 transcription and reducing myocardial aldosterone synthesis.
DOI: 10.6084/m9.figshare.8848088.v1
title: Changes in cardiac aldosterone and its synthase in rats with chronic heart failure: an intervention study of long-term treatment with recombinant human brain natriuretic peptide

description: The physiological mechanisms involved in isoproterenol (ISO)-induced chronic heart failure (CHF) are not fully understood. In this study, we investigated local changes in cardiac aldosterone and its synthase in rats with ISO-induced CHF, and evaluated the effects of treatment with recombinant human brain natriuretic peptide (rhBNP). Sprague-Dawley rats were divided into 4 different groups. Fifty rats received subcutaneous ISO injections to induce CHF and the control group (n=10) received equal volumes of saline. After establishing the rat model, 9 CHF rats received no further treatment, rats in the low-dose group (n=8) received 22.5 μg/kg rhBNP and those in the high-dose group (n=8) received 45 μg/kg rhBNP daily for 1 month. Cardiac function was assessed by echocardiographic and hemodynamic analysis. Collagen volume fraction (CVF) was determined. Plasma and myocardial aldosterone concentrations were determined using radioimmunoassay. Myocardial aldosterone synthase (CYP11B2) was detected by quantitative real-time PCR. Cardiac function was significantly lower in the CHF group than in the control group (P&lt;0.01), whereas CVF, plasma and myocardial aldosterone, and CYP11B2 transcription were significantly higher than in the control group (P&lt;0.05). Low and high doses of rhBNP significantly improved hemodynamics (P&lt;0.01) and cardiac function (P&lt;0.05) and reduced CVF, plasma and myocardial aldosterone, and CYP11B2 transcription (P&lt;0.05). There were no significant differences between the rhBNP dose groups (P&gt;0.05). Elevated cardiac aldosterone and upregulation of aldosterone synthase expression were detected in rats with ISO-induced CHF. Administration of rhBNP improved hemodynamics and ventricular remodeling and reduced myocardial fibrosis, possibly by downregulating CYP11B2 transcription and reducing myocardial aldosterone synthesis.
DOI: 10.6084/m9.figshare.8848070
title: Official meteorological station data underestimate the heat stress on dairy cattle in a tropical environment

description: ABSTRACT The values of air temperature, relative humidity and Temperature Humidity Index (THI) of one pasture-based milk production farm and from the nearest official weather station were confronted. At the farm, data readings were recorded by an automatic weather station, every five minutes, from February 2015 to September 2016. Then, the hourly average and hourly THI were calculated. The minimum, average, and maximum daily values were selected. The same was done with data from the official hourly weather station data. The minimum and mean temperature in the meteorological station were higher than on the farm throughout the experimental period and in 16 months, respectively. The maximum temperature of the rural property was greater than that of the station in six months. Meanwhile, the minimum, average, and maximum relative humidity of the meteorological station were lower than those of the farm. The minimum THI was higher at the official station and the maximum THI was higher at the farm in every month. Finally, the number of days with a maximum THI equal to or greater than the critical THI was greater on the farm. Thus, official weather station data underestimate the heat stress.
DOI: 10.6084/m9.figshare.8848070.v1
title: Official meteorological station data underestimate the heat stress on dairy cattle in a tropical environment

description: ABSTRACT The values of air temperature, relative humidity and Temperature Humidity Index (THI) of one pasture-based milk production farm and from the nearest official weather station were confronted. At the farm, data readings were recorded by an automatic weather station, every five minutes, from February 2015 to September 2016. Then, the hourly average and hourly THI were calculated. The minimum, average, and maximum daily values were selected. The same was done with data from the official hourly weather station data. The minimum and mean temperature in the meteorological station were higher than on the farm throughout the experimental period and in 16 months, respectively. The maximum temperature of the rural property was greater than that of the station in six months. Meanwhile, the minimum, average, and maximum relative humidity of the meteorological station were lower than those of the farm. The minimum THI was higher at the official station and the maximum THI was higher at the farm in every month. Finally, the number of days with a maximum THI equal to or greater than the critical THI was greater on the farm. Thus, official weather station data underestimate the heat stress.
DOI: 10.6084/m9.figshare.8848055
title: No fitness costs are induced by Spiroplasma infections of Aphis citricidus reared on two different host plants

description: Abstract Aphids can harbor several secondary symbionts that alter important aphid-related ecological traits, such as defense against natural enemies, heat tolerance and host plant utilization. One of these secondary symbionts, Spiroplasma, is well known in Drosophila as a sex modulator and by interacting with the host immune system. However, little is known on the effects of Spiroplasma on aphids, such as its influence on the host immune defense against fungi and on host plant utilization. Aphid infections by Spiroplasma are known to be low and few aphid species were reported to be infected with this secondary symbiont, however aphids belonging to the genus Aphis in neotropical regions show high infection rates by Spiroplasma. Thus, we investigated the association of Spiroplasma with the tropical aphid Aphis citricidus through comparative biology experiments on two host plants with different nutritional value to the aphid. We demonstrate Spiroplasma induced no significant fitness costs to A. citricidus on either host plant as no changes in the fitness traits we assessed were observed. Spiroplasma infection only induced sutle changes on host longevity and fecundity. Therefore, we concluded Spiroplasma established a neutral interaction with A. citricidus under the selection pressure we tested, and argue on stress conditions that could better demonstrate the role of Spiroplasma in A. citricidus bioecology and associated costs involved.
DOI: 10.6084/m9.figshare.8848058
title: Conservation of Vriesea flammea L.B.Sm., an endemic Brazilian bromeliad: effects of nutrients and carbon source on plant development

description: Abstract Bromeliaceae is restricted to the Neotropical region and has a high degree of endemism, which contributes to increased biodiversity because of the diverse morphological characteristics of individuals. In order to develop an in vitro conservation technology to obtain plants for reintroduction, seeds of Vriesea flammea L.B.Sm. were collected, sterilized and germinated in culture medium. The plants obtained were cultured for 180 days in MS medium with different concentrations of mineral nutrients (25 and 50% of nitrogenous salts and macronutrients), and different concentrations of sucrose (20, 30, 40, 50 and 60 g L-1), and then acclimatized for 150 days on commercial substrate. When seeds were sterilized directly, only 4% of them were contaminated, whereas sterilization of capsules resulted in 43.6% contaminated seeds. Germination rates above 80% were recorded. Low concentrations of nitrogenous salts and macronutrients produced greater than 76% survival and promoted greater in vitro plant development than the complete MS medium. The development of the aerial system, root system, fresh mass and photosynthetic pigments were positively related to sucrose concentration in vitro. The highest sucrose concentration also indirectly promoted greater development of the aerial system and fresh mass of acclimatized plants. We established conditions for in vitro cultivation and acclimatization for efficient propagation of V. flammea with a view towards conservation of the species or reestablishment of natural populations.
DOI: 10.6084/m9.figshare.8848049
title: Feasibility of wood production according to productivity class and rotation criteria

description: Abstract: The objective of this work was to evaluate the effect of forest rotation on the technical and economic feasibility of eucalyptus wood production for charcoal, in different productivity classes. Data came from Eucalyptus stands whose area was stratified into three classes of productivity: high, medium, and low. To each class, a different criterion of forest rotation was applied, as follows: age of maximum productivity, economic rotation age, single harvest age, and no technical parameter. Analyses of economic feasibility and production were performed for a 21-year planning horizon. For rotation without a technical parameter, a simulation by the Monte Carlo method was performed. For all rotation criteria, feasibility was observed for high- and medium-productivity classes, and unfeasibility for the low-productivity class. For rotation without a technical parameter, there is no chance for the project to be unfeasible. Wood production viability for charcoal under the studied conditions depends on the productivity class, for any rotation criterion adopted. Rotation criterion influences profitability, whose definition, according to productivity class, contributes to forest production maximization and to the economic return of the project, under the studied conditions.
DOI: 10.6084/m9.figshare.8848052
title: Canine cavitary effusion: a retrospective study of 304 cases in Brazil

description: ABSTRACT The objective of this study was to determine the frequency of different categories of specific and general classification in canine cavitary effusions (CE), as well as their association with the underlying etiologies. The laboratorial and clinical data from 304 cases of canine CE were retrospectively assessed. In 32.9% (100 cases), at least one of the specific classification categories was established, with a subtotal predominance of neoplasia (42%), bacterial serositis (24%) and hemorrhage (16%). Neoplasia was confirmed by effusion cytology in 57.5% of the cases with histopathological confirmation. From the cases in which the specific classification was not obtained, 35.8% were classified as modified transudate, 30.4% as pure transudate, 21.1% % as exudate and 12.7% was not included in any general category. The most common causes of effusion among these cases were hypoproteinemia and/or hipoalbuminemia (HPHA) (25.8%), hepatopathy (22.5%), cardiac insufficiency (15.5%) and cytologically undetected cases of neoplasia (12.4%). In conclusion, HPHA, hepatopathy and neoplasia represents important etiologies for canine CE development. Classification of effusions, solely based on [TP] and TNCC, might be an inaccurate diagnostic tool of effusions. New laboratorial classification methods for canine CE should be researched.
DOI: 10.6084/m9.figshare.8848055.v1
title: No fitness costs are induced by Spiroplasma infections of Aphis citricidus reared on two different host plants

description: Abstract Aphids can harbor several secondary symbionts that alter important aphid-related ecological traits, such as defense against natural enemies, heat tolerance and host plant utilization. One of these secondary symbionts, Spiroplasma, is well known in Drosophila as a sex modulator and by interacting with the host immune system. However, little is known on the effects of Spiroplasma on aphids, such as its influence on the host immune defense against fungi and on host plant utilization. Aphid infections by Spiroplasma are known to be low and few aphid species were reported to be infected with this secondary symbiont, however aphids belonging to the genus Aphis in neotropical regions show high infection rates by Spiroplasma. Thus, we investigated the association of Spiroplasma with the tropical aphid Aphis citricidus through comparative biology experiments on two host plants with different nutritional value to the aphid. We demonstrate Spiroplasma induced no significant fitness costs to A. citricidus on either host plant as no changes in the fitness traits we assessed were observed. Spiroplasma infection only induced sutle changes on host longevity and fecundity. Therefore, we concluded Spiroplasma established a neutral interaction with A. citricidus under the selection pressure we tested, and argue on stress conditions that could better demonstrate the role of Spiroplasma in A. citricidus bioecology and associated costs involved.
DOI: 10.6084/m9.figshare.8848058.v1
title: Conservation of Vriesea flammea L.B.Sm., an endemic Brazilian bromeliad: effects of nutrients and carbon source on plant development

description: Abstract Bromeliaceae is restricted to the Neotropical region and has a high degree of endemism, which contributes to increased biodiversity because of the diverse morphological characteristics of individuals. In order to develop an in vitro conservation technology to obtain plants for reintroduction, seeds of Vriesea flammea L.B.Sm. were collected, sterilized and germinated in culture medium. The plants obtained were cultured for 180 days in MS medium with different concentrations of mineral nutrients (25 and 50% of nitrogenous salts and macronutrients), and different concentrations of sucrose (20, 30, 40, 50 and 60 g L-1), and then acclimatized for 150 days on commercial substrate. When seeds were sterilized directly, only 4% of them were contaminated, whereas sterilization of capsules resulted in 43.6% contaminated seeds. Germination rates above 80% were recorded. Low concentrations of nitrogenous salts and macronutrients produced greater than 76% survival and promoted greater in vitro plant development than the complete MS medium. The development of the aerial system, root system, fresh mass and photosynthetic pigments were positively related to sucrose concentration in vitro. The highest sucrose concentration also indirectly promoted greater development of the aerial system and fresh mass of acclimatized plants. We established conditions for in vitro cultivation and acclimatization for efficient propagation of V. flammea with a view towards conservation of the species or reestablishment of natural populations.
DOI: 10.6084/m9.figshare.8848049.v1
title: Feasibility of wood production according to productivity class and rotation criteria

description: Abstract: The objective of this work was to evaluate the effect of forest rotation on the technical and economic feasibility of eucalyptus wood production for charcoal, in different productivity classes. Data came from Eucalyptus stands whose area was stratified into three classes of productivity: high, medium, and low. To each class, a different criterion of forest rotation was applied, as follows: age of maximum productivity, economic rotation age, single harvest age, and no technical parameter. Analyses of economic feasibility and production were performed for a 21-year planning horizon. For rotation without a technical parameter, a simulation by the Monte Carlo method was performed. For all rotation criteria, feasibility was observed for high- and medium-productivity classes, and unfeasibility for the low-productivity class. For rotation without a technical parameter, there is no chance for the project to be unfeasible. Wood production viability for charcoal under the studied conditions depends on the productivity class, for any rotation criterion adopted. Rotation criterion influences profitability, whose definition, according to productivity class, contributes to forest production maximization and to the economic return of the project, under the studied conditions.
DOI: 10.6084/m9.figshare.8848052.v1
title: Canine cavitary effusion: a retrospective study of 304 cases in Brazil

description: ABSTRACT The objective of this study was to determine the frequency of different categories of specific and general classification in canine cavitary effusions (CE), as well as their association with the underlying etiologies. The laboratorial and clinical data from 304 cases of canine CE were retrospectively assessed. In 32.9% (100 cases), at least one of the specific classification categories was established, with a subtotal predominance of neoplasia (42%), bacterial serositis (24%) and hemorrhage (16%). Neoplasia was confirmed by effusion cytology in 57.5% of the cases with histopathological confirmation. From the cases in which the specific classification was not obtained, 35.8% were classified as modified transudate, 30.4% as pure transudate, 21.1% % as exudate and 12.7% was not included in any general category. The most common causes of effusion among these cases were hypoproteinemia and/or hipoalbuminemia (HPHA) (25.8%), hepatopathy (22.5%), cardiac insufficiency (15.5%) and cytologically undetected cases of neoplasia (12.4%). In conclusion, HPHA, hepatopathy and neoplasia represents important etiologies for canine CE development. Classification of effusions, solely based on [TP] and TNCC, might be an inaccurate diagnostic tool of effusions. New laboratorial classification methods for canine CE should be researched.
DOI: 10.6084/m9.figshare.8848043
title: Effects of Acute Physical Exercise and Television Viewing on Mood in Older Active Women

description: Abstract Aim: Due to the increased prevalence of mood disorders and the detrimental effects of sedentary behavior on health, a better understanding of the association between physical exercise (PE), TV viewing and mood in older adults is paramount. This is based on the fact that mood has been recognized as an important predictor of longevity, well-being, and quality of life. This study examined the effects of an acute bout of physical exercise (PE) and television viewing (TV) on positive and negative mood states of older active women. Methods: Fifty-four women aged 60 years and older were asked to complete the Abbreviated and Illustrated Mood States Inventory (LEA-RI) before and immediatly after two distinct activities: 1) single bout of PE; and 2) a single bout of TV. Participants further completed questionnaires to provide information on demographics, physical activity and sedentary behavior for sample characterization. Differences between conditions (PE and TV) on positive and negative mood state were analyzed using repeated measures ANOVA followed by paired sample t-test adopting a significance level of P
DOI: 10.6084/m9.figshare.8848037
title: Intercostal celiotomy for removal of proventriculus foreign body in muscovy duck (Cairina moschata): case report

description: ABSTRACT Ducks, geese and swans are included in the Anatidae family, Anseriformes order. The leading injuries causes to waterfowl are tangling in fishing materials and foreign bodies ingestion. A muscovy duck (Cairina moschata) was referred for treatment at Veterinary Teaching Hospital and a radiographic examination showed the presence of a hook in the coelom. Surgical exposure and incision of the proventriculus was made through left intercostal access and the hook along with a fishing line were gently removed. The animal began to feed voluntarily at the fourth post-operative day and two weeks after the procedure the patient was clinically well and was released to wild. This surgical approach differs in some aspects from the listed techniques in the known literature. It proved to be a viable and appropriate alternative to treat this affection since it did not cause any trans- or post-operative complications and enabled rapid recovery and subsequent patient release.
DOI: 10.6084/m9.figshare.8848040
title: Increased metabolisable energy from glycerine or soybean oil in broilers

description: ABSTRACT The effect of increased levels of metabolisable energy with glycerine or soybean oil was evaluated in the performance of broilers at four growth stages (1 to 7, 8 to 21, 22 to 33 and 34 to 42 days), in addition to the carcass and cut yield. A total of 2,400 birds were used in a completely randomised design in a 2 x 4 factorial scheme (two sources x four energy levels), with five replications. The treatments were determined by including glycerine or soybean oil at intervals of 75 kcal/kg of metabolisable energy in the diet: the first level with 75 kcal less than the requirement, the second corresponding to the requirement for each stage (2925, 2980, 3050 and 3100 Kcal), and the third and fourth levels with 75 kcal and 150 kcal more than the required value for the stage under evaluation. The metabolisable energy requirement for broilers, irrespective of the energy source (glycerine or soybean oil), was estimated at 3,075, 3.130, 3,200 and 3,250 kcal ME/kg feed, for stages 1 to 7, 8 to 21, 22 to 33 and 34 to 42 days respectively. However, the use of glycerine as a source of increased energy promoted better performance efficiency during the first two stages (1 to 7 and 8 to 21 days of age).