DOI: 10.5061/dryad.msbcc2fvx
title: Data from: A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus

description: Sheeppox is a transboundary disease of sheep caused by infection with the
 capripoxvirus sheeppox virus (SPPV). Sheeppox is found in Africa, the
 Middle East and Asia and is characterised by fever, multifocal cutaneous
 raised lesions, and death, with substantial negative impact on affected
 flocks. Vaccination with live attenuated capripoxvirus (CPPV) strains is
 an effective and widely used means of controlling sheeppox outbreaks,
 however there are few reports of post-vaccination field surveillance
 studies of sheeppox. This study used a commercially available ELISA and a
 fluorescence-based neutralisation assay (FVNT) to examine quantitative and
 temporal features of the humoral response of sheep vaccinated with a live
 attenuated CPPV strain in Mongolia. 400 samples were tested using the
 ELISA, and a subset of 45 also tested with the FVNT. There was substantial
 agreement between the FVNT and ELISA tests. Antibodies to CPPV were
 detected between 40 and 262 days post vaccination. There was no
 significant difference between serological status (positive / negative)
 and sex or age, however an inverse correlation was found between the
 length of time since vaccination and serological status. Animals between
 90 and 180 days post-vaccination were more likely to be positive than
 animals greater than 180 days post vaccination. This data provides
 temporal parameters to consider when planning sheeppox post-vaccination
 monitoring programmes. In summary, our results show a commercial CPPV
 ELISA kit is a robust and reliable assay for use in resource-restricted
 low and low-middle income countries for post CPPV vaccination surveillance
 on a regional or national level.
description: Blood samples from sheep
 and associated data were collected as part of the post-vaccination
 surveillance programme for sheeppox implemented by the Mongolian General
 Authority for Veterinary Services (GAVS) in 2016.
description: The attached .xls file contains all raw data used in the
 associated publication, " A comparative serological field study
 evaluating the humoral immune response in Mongolian sheep vaccinated
 against sheeppox virus." The dataset identifies all individual sheep
 by a unique identification number (ID_num). Each unique ID_num is
 associated with relevant metadata: Province name, Sum name, herder name
 coded (Herded_Id), animal species, age of the animal, in years, when
 sampled (Age), sex of the animal “F” or “M” (Sex), date when the animal
 was sampled (Date_sample_collected), date when the animal was vaccinated
 for sheeppox according to the vaccination records (Date_vaccinated_raw
 data), and for samples in which the exact date of vaccination was not
 available and a range of potential time was given, the midpoint date
 within this range (Date_vaccinated_midpoint), animals for which
 vaccination date was not available receive an "NA" value; time
 from vaccination to sampled in days (Time_from_vaccinated_midpoint);  %S/P
 values for the ELISA test conducted in the Mongolia lab
 (Ag_ELISA_SCVL_OD_SP) and %S/P values for the ELISA test conducted in the
 UK lab (Ag_ELISA_TPI_OD_SP), results from the ELISA test classified as
 binary variable “Positive” or “Negative” (Ag_ELISA_SCVL_bin and
 Ag_ELISA_TPI_bin); titres from the fluorescence-based neutralisation assay
 (FVNT Titre) and results from the FVNT test classified as binary variable
 (“Positive” or “Negative”), all animals that were not tested by FVNT
 receive an "NA" value in these columns.