cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.13289777
title: Data on real time PCR and K9 dogs for the assessment of COVID-19 infection in human population

description: This data record contains a single dataset, <b>K9_COVID19 Anonymized Data 20.11.2020 - 1215.xlsx</b>, in .<b>xlsx</b> file format. The file contains the data output for two tests, K9 dogs and quantitative reverse-transcription polymerase chain reaction (RT-PCR), for the detection of SARS-CoV-2.<b> </b> Data are provided for the 884 human research participants included in the study. Each row in the data file includes a code, age, geographical origin (either African or Asian), result of the RT-PCR test for COVID-19 and result of the K9 test for COVID-19.<br> <b> </b> <b>Research participant consent</b>: Individuals participating in the study provided their written consent.<br> <b>Study aims and methodology:</b> In January 2020, the coronavirus disease was declared, by the World Health Organization as a global public health emergency. Recent recommendations from the WHO COVID Emergency Committee continue to support strengthening COVID surveillance systems, including timely access to effective diagnostics. Questions were raised about the validity of considering the RT-PCR as the gold standard in COVID-19 diagosis3. It has been suggested that a variety of methods should be used to evaluate advocated tests. Dogs had been successfully trained and employed to detect diseases in humans. The aims of this study are: (1) To use the RT-PCR test as the gold standard to evaluate the ability of police detection dogs (K9 dogs) to screen for SARS-CoV-2 using axillary sweat samples and (2) To compare the diagnostic accuracy of the K9 dog olfaction and the real-time PCR tests using Bayesian Latent Class Analysis. Two types of samples were collected from different persons; one for training the dogs and the other was for the comparative studies. Sweat samples (n=234) were collected from arm pits of COVID-19 patients using a sterile gauze pad at a hospital in Abu Dhabi, UAE. These patients showed respiratory manifestations and were identified by reverse transcription–polymerase chain reaction (RT-PCR) as COVID-19 positive using nasal swabs. Negative control sweat samples (n=144) were collected from persons that did not show any recent respiratory manifestations and their nasal swabs tested negative for SARS-CoV-2 by RT-PCR. A total of 884-arm pit sweat samples were collected from arm pits of individuals visiting the COVID-19 screening center in Mafraq Workers City, Abu Dhabi, UAE. The samples were subjected to screening using trained K9 dogs. Nasal swabs from the same individuals involved in the study were tested by SARS-CoV-2-RT-PCR protocol to identify COVID-19 positive and negative patients. For more details on the methodology, including Bayesian Latent Class Analysis and statistical analysis, please refer to the related article.<br>
DOI: 10.6084/m9.figshare.13289777.v1
title: Data on real time PCR and K9 dogs for the assessment of COVID-19 infection in human population

description: This data record contains a single dataset, <b>K9_COVID19 Anonymized Data 20.11.2020 - 1215.xlsx</b>, in .<b>xlsx</b> file format. The file contains the data output for two tests, K9 dogs and quantitative reverse-transcription polymerase chain reaction (RT-PCR), for the detection of SARS-CoV-2.<b> </b> Data are provided for the 884 human research participants included in the study. Each row in the data file includes a code, age, geographical origin (either African or Asian), result of the RT-PCR test for COVID-19 and result of the K9 test for COVID-19.<br> <b> </b> <b>Research participant consent</b>: Individuals participating in the study provided their written consent.<br> <b>Study aims and methodology:</b> In January 2020, the coronavirus disease was declared, by the World Health Organization as a global public health emergency. Recent recommendations from the WHO COVID Emergency Committee continue to support strengthening COVID surveillance systems, including timely access to effective diagnostics. Questions were raised about the validity of considering the RT-PCR as the gold standard in COVID-19 diagosis3. It has been suggested that a variety of methods should be used to evaluate advocated tests. Dogs had been successfully trained and employed to detect diseases in humans. The aims of this study are: (1) To use the RT-PCR test as the gold standard to evaluate the ability of police detection dogs (K9 dogs) to screen for SARS-CoV-2 using axillary sweat samples and (2) To compare the diagnostic accuracy of the K9 dog olfaction and the real-time PCR tests using Bayesian Latent Class Analysis. Two types of samples were collected from different persons; one for training the dogs and the other was for the comparative studies. Sweat samples (n=234) were collected from arm pits of COVID-19 patients using a sterile gauze pad at a hospital in Abu Dhabi, UAE. These patients showed respiratory manifestations and were identified by reverse transcription–polymerase chain reaction (RT-PCR) as COVID-19 positive using nasal swabs. Negative control sweat samples (n=144) were collected from persons that did not show any recent respiratory manifestations and their nasal swabs tested negative for SARS-CoV-2 by RT-PCR. A total of 884-arm pit sweat samples were collected from arm pits of individuals visiting the COVID-19 screening center in Mafraq Workers City, Abu Dhabi, UAE. The samples were subjected to screening using trained K9 dogs. Nasal swabs from the same individuals involved in the study were tested by SARS-CoV-2-RT-PCR protocol to identify COVID-19 positive and negative patients. For more details on the methodology, including Bayesian Latent Class Analysis and statistical analysis, please refer to the related article.<br>
DOI: 10.5281/zenodo.4772750
title: Iimbovane Outreach Project: Soil properties

description: The Iimbovane project implemented a long term biodiversity monitoring programme in the Western Cape Province of South Africa. Starting in 2006 the project proposes to continue for 10 years. The project monitors ant assemblages from 33 sites to examine their diversity patterns over large spatial and temporal scales. During 2006 the project also collected soil samples to determine the soil characteristics at the research sites for a better understanding of the soil structure preferences of the ant species present.
description: This data set provides the user with soil properties of research sites sampled by the project. The data includes variables used for soil classification and soil chemistry.
DOI: 10.5281/zenodo.4772719
title: Iimbovane Outreach Project: Ant data

description: The Iimbovane Outreach Project investigates the abundance and diversity of ants along two extensive transects through the Fynbos and Succulent Karoo biomes of South Africa. It is a long term project initiated in 2005 by Principal Investigator Prof. S.L. Chown, Stellenbosch University. Data collection is carried out by Grade 10 learners and the Iimbovane team on a biannual (spring and autumn) basis.
description: To investigate changes in invertebrate assemblages, specifically ants, in space and over time in different natural and modified landscapes of the Western Cape.
DOI: 10.5281/zenodo.4772718
title: Iimbovane Outreach Project: Ant data

description: The Iimbovane Outreach Project investigates the abundance and diversity of ants along two extensive transects through the Fynbos and Succulent Karoo biomes of South Africa. It is a long term project initiated in 2005 by Principal Investigator Prof. S.L. Chown, Stellenbosch University. Data collection is carried out by Grade 10 learners and the Iimbovane team on a biannual (spring and autumn) basis.
description: To investigate changes in invertebrate assemblages, specifically ants, in space and over time in different natural and modified landscapes of the Western Cape.
DOI: 10.5281/zenodo.4772738
title: Iimbovane Outreach Project: Related Information

description: The Iimbovane project implemented a long term biodiversity monitoring programme in the Western Cape Province of South Africa. Starting in 2006 the project proposes to continue for 10 years. The project monitors ant assemblages from 33 sites to examine their diversity patterns over large spatial and temporal scales. Additionally, the programme aims to build capacity and knowledge in the field of invertebrate biodiversity amongst secondary school-level educators and learners by exposing learners to biodiversity sampling techniques. Learners assist scientists with collecting invertebrate and vegetation data by means of pitfalls and vegetation surveys.
description: The project aims to monitor ant diversity through space and time that would contribute to an inventory programme for invertebrates and to the overall framework for national biodiversity monitoring. Set in the major biomes of the Western Cape, the project improves the current understanding of ant composition in the different biomes. The monitoring programme also monitors the arrival of new alien species and assesses the changes in distribution of established alien species.
DOI: 10.5281/zenodo.4772737
title: Iimbovane Outreach Project: Related Information

description: The Iimbovane project implemented a long term biodiversity monitoring programme in the Western Cape Province of South Africa. Starting in 2006 the project proposes to continue for 10 years. The project monitors ant assemblages from 33 sites to examine their diversity patterns over large spatial and temporal scales. Additionally, the programme aims to build capacity and knowledge in the field of invertebrate biodiversity amongst secondary school-level educators and learners by exposing learners to biodiversity sampling techniques. Learners assist scientists with collecting invertebrate and vegetation data by means of pitfalls and vegetation surveys.
description: The project aims to monitor ant diversity through space and time that would contribute to an inventory programme for invertebrates and to the overall framework for national biodiversity monitoring. Set in the major biomes of the Western Cape, the project improves the current understanding of ant composition in the different biomes. The monitoring programme also monitors the arrival of new alien species and assesses the changes in distribution of established alien species.
DOI: 10.5281/zenodo.4772734
title: Iimbovane Outreach Project: Geospatial Information File

description: The Iimbovane Outreach Project investigates the abundance and diversity of ants along two extensive transects through the Fynbos and Succulent Karoo biomes of South Africa. It is a long term project initiated in 2005 by Principal Investigator Prof. S.L. Chown, Stellenbosch University. Data collection is carried out by learners on a biannual (spring and autumn) basis in collaboration.
description: To provide information on the location and history of Iimbovane long-term monitoring research sites.
DOI: 10.5281/zenodo.4772733
title: Iimbovane Outreach Project: Geospatial Information File

description: The Iimbovane Outreach Project investigates the abundance and diversity of ants along two extensive transects through the Fynbos and Succulent Karoo biomes of South Africa. It is a long term project initiated in 2005 by Principal Investigator Prof. S.L. Chown, Stellenbosch University. Data collection is carried out by learners on a biannual (spring and autumn) basis in collaboration.
description: To provide information on the location and history of Iimbovane long-term monitoring research sites.
DOI: 10.5281/zenodo.4772727
title: Iimbovane Outreach Project: Climate data

description: The Iimbovane outreach project investigates the abundance and diversity of ants along two extensive transects through the Fynbos and Succulent Karoo biomes of South Africa. It is a long term project initiated in 2005 by Principal Investigator Prof. S.L. Chown, Stellenbosch University. Data collection is carried out learners on a biannual (spring and autumn) basis in collaboration with Grade 10 learners.
description: To monitor changes in invertebrate assemblages, focusing on ants. This file contains the first temperature data which were collected from the Iimbovane sites.
DOI: 10.5281/zenodo.4772726
title: Iimbovane Outreach Project: Climate data

description: The Iimbovane outreach project investigates the abundance and diversity of ants along two extensive transects through the Fynbos and Succulent Karoo biomes of South Africa. It is a long term project initiated in 2005 by Principal Investigator Prof. S.L. Chown, Stellenbosch University. Data collection is carried out learners on a biannual (spring and autumn) basis in collaboration with Grade 10 learners.
description: To monitor changes in invertebrate assemblages, focusing on ants. This file contains the first temperature data which were collected from the Iimbovane sites.
DOI: 10.5281/zenodo.4772712
title: Cederberg Vegetation Height Data

description: The Coast to Karoo Transect in the Cederberg investigates the abundance and species diversity of Ants and Beetles along the altitudinal line. It is a long term project that also aim to show some changes with Climate Change. These files on vegetation data provide micro-habitat information which aim to put the invertebrate data in contects.
description: To Monitor the long term change in insect assemblages in the Cederberg, focusing on ants and beetles. Currentley more than 90 species have been recorded.
DOI: 10.5281/zenodo.4772711
title: Cederberg Vegetation Height Data

description: The Coast to Karoo Transect in the Cederberg investigates the abundance and species diversity of Ants and Beetles along the altitudinal line. It is a long term project that also aim to show some changes with Climate Change. These files on vegetation data provide micro-habitat information which aim to put the invertebrate data in contects.
description: To Monitor the long term change in insect assemblages in the Cederberg, focusing on ants and beetles. Currentley more than 90 species have been recorded.
DOI: 10.5281/zenodo.4772704
title: Cederberg Vegetation Cover Data

description: The Coast to Karoo Transect in the Cederberg investigates the abundance and species diversity of ants and beetles along the altitudinal line. It is a long term project that might also show some changes with Climate Change. These files on Vegetation data provide micro-habitat information which aim to put the invertabrate data in contects.
description: To monitor the long term change in insect assemblages in the Cederberg, focusing on ants and beetles. Currently more than 90 species have been recorded.
DOI: 10.5281/zenodo.4772703
title: Cederberg Vegetation Cover Data

description: The Coast to Karoo Transect in the Cederberg investigates the abundance and species diversity of ants and beetles along the altitudinal line. It is a long term project that might also show some changes with Climate Change. These files on Vegetation data provide micro-habitat information which aim to put the invertabrate data in contects.
description: To monitor the long term change in insect assemblages in the Cederberg, focusing on ants and beetles. Currently more than 90 species have been recorded.
DOI: 10.5281/zenodo.4772700
title: Cederberg Notes on Pitfalls and GeoSpatial Data

description: The Coast to Karoo Transect in the Cederberg investigates the abundance and species diversity of ants and beetles along the altitudinal line. Currently more than 90 species have been recorded. It is a long term project that aim to show some changes with Climate Change.
description: To monitor the long-term change in insect assemblages in the Cederberg, focusing on ants and beetles. This file provide addoitional information on GeoSpacial data, Fire data for the area and pitfall disturbance recorded.
DOI: 10.5281/zenodo.4772699
title: Cederberg Notes on Pitfalls and GeoSpatial Data

description: The Coast to Karoo Transect in the Cederberg investigates the abundance and species diversity of ants and beetles along the altitudinal line. Currently more than 90 species have been recorded. It is a long term project that aim to show some changes with Climate Change.
description: To monitor the long-term change in insect assemblages in the Cederberg, focusing on ants and beetles. This file provide addoitional information on GeoSpacial data, Fire data for the area and pitfall disturbance recorded.
DOI: 10.6084/m9.figshare.12473036.v2
title: Resource-dependent fitness effects of MA lines at different temperatures

description: All the 10 genotypes derive from a mutation accumulation (MA) experiment with <i>Eshcerichia coli</i> B REL 606 mutS. Growth performance of each of the MA lines was estimated by the growth yield at stationary phase and calibrated by the ancestral strain. The assays were carried out on six carbon-substrates (fructose, galactose, glucose, glycerol, maltose and trehalose) across 10 temperatures (23, 25, 27, 29, 31, 33, 35, 37, 39 and 41°C). File data.temp.niche.xlsx gives growth performance data ,where Sheet 1 gives the mean values and Sheet 2 shows values with all three technical replicates. File SNP_info.xlsx gives the information of each SNP mutation.
DOI: 10.6084/m9.figshare.12473036.v1
title: Resource-dependent fitness effects of MA lines at different temperatures

description: All the 10 genotypes derive from a mutation accumulation (MA) experiment with <i>Eshcerichia coli</i> B REL 606 mutS. Growth performance of each of the MA lines was estimated by the growth yield at stationary phase and calibrated by the ancestral strain. The assays were carried out on six carbon-substrates (fructose, galactose, glucose, glycerol, maltose and trehalose) across 10 temperatures (23, 25, 27, 29, 31, 33, 35, 37, 39 and 41°C). Sheet 1 gives the mean values and Sheet 2 shows values with all three technical replicates.
DOI: 10.6084/m9.figshare.12473036
title: Resource-dependent fitness effects of MA lines at different temperatures

description: All the 10 genotypes derive from a mutation accumulation (MA) experiment with <i>Eshcerichia coli</i> B REL 606 mutS. Growth performance of each of the MA lines was estimated by the growth yield at stationary phase and calibrated by the ancestral strain. The assays were carried out on six carbon-substrates (fructose, galactose, glucose, glycerol, maltose and trehalose) across 10 temperatures (23, 25, 27, 29, 31, 33, 35, 37, 39 and 41°C). File data.temp.niche.xlsx gives growth performance data ,where Sheet 1 gives the mean values and Sheet 2 shows values with all three technical replicates. File SNP_info.xlsx gives the information of each SNP mutation.
DOI: 10.5281/zenodo.4772692
title: Cederberg Beetles Data

description: The main aim of the project is to determine the abundance and species diversity of ants and beetles along the altitudinal transect (Cederberg transect). The transect comprises of 17 sampling sites, each with 4 plots from the west coast (Lambert's Bay; sea level) to Wupperthal (Karoo) crossing the Cederberg mountains including Sneeukop Peak (1926 m). The sites are sampled twice a year using pitfall traps.
description: Was to investigate the change of body size in both males and females specimens per altitude in different years.
DOI: 10.5281/zenodo.4772691
title: Cederberg Beetles Data

description: The main aim of the project is to determine the abundance and species diversity of ants and beetles along the altitudinal transect (Cederberg transect). The transect comprises of 17 sampling sites, each with 4 plots from the west coast (Lambert's Bay; sea level) to Wupperthal (Karoo) crossing the Cederberg mountains including Sneeukop Peak (1926 m). The sites are sampled twice a year using pitfall traps.
description: Was to investigate the change of body size in both males and females specimens per altitude in different years.
DOI: 10.5281/zenodo.4772773
title: Soutpansberg Climate Data

description: The Soutpansberg Transect investigates the abundance and diversity of ants and spiders along an altitudinal gradient in the Western Soutpansberg Mountains, South Africa. It has a north-south orientation over the mountain and is a long term project initiated in 2009. Data collection is carried out in intervals that suit the data logger capacity.
description: To study the diversity patterns in ants and other invertebrate groups across the spatial transect over a long-term period and the climate the invertebrates.
DOI: 10.5281/zenodo.4772772
title: Soutpansberg Climate Data

description: The Soutpansberg Transect investigates the abundance and diversity of ants and spiders along an altitudinal gradient in the Western Soutpansberg Mountains, South Africa. It has a north-south orientation over the mountain and is a long term project initiated in 2009. Data collection is carried out in intervals that suit the data logger capacity.
description: To study the diversity patterns in ants and other invertebrate groups across the spatial transect over a long-term period and the climate the invertebrates.
DOI: 10.6084/m9.figshare.14625805
title: Inhibitory effect of short-term ketogenic diet on cytokine expression during orthodontic movement in a rat model

description: <b>Purpose:</b> To compare the change of IL-1β and TNF-α expression during orthodontic tooth movement in rats, which were under regular diet and ketogenic diet (KD). <b>Materials and Methods:</b> The maxillary right first molars of 3-month-old male Sprague-Dawley rats were subjected to mesial movement by 50 g of force for 14 days under ketogenic diet. A control group was also established in which rats were subjected to the same tooth movement conditions but under a regular diet. The distance of tooth movement was measured by using precision electronic calipers. After 14 days, the rats were sacrificed and the mesial surface of the palatal root of the right first molar was analysed to determine the expression levels of IL-1β and TNF-α through immunohistochemical staining. The images obtained were then quantified using ImageJ program. <b>Results:</b> After 14 days of orthodontic tooth movement, the amount of tooth movement in the ketogenic group was lesser by approximately half the distance compared to that of the control group. Moreover, the expression of IL-1β and TNF-α was also lower. <b>Conclusions:</b> Consuming ketogenic diet reduces the expected amount of tooth movement which was evidently shown by a significant lower cytokine expression of IL-1β and TNF-α than the group under regular diet. This suggests that ketogenic diet is a contributing factor in delaying the tooth movement during orthodontic treatment.
DOI: 10.6084/m9.figshare.14625805.v1
title: Inhibitory effect of short-term ketogenic diet on cytokine expression during orthodontic movement in a rat model

description: <b>Purpose:</b> To compare the change of IL-1β and TNF-α expression during orthodontic tooth movement in rats, which were under regular diet and ketogenic diet (KD). <b>Materials and Methods:</b> The maxillary right first molars of 3-month-old male Sprague-Dawley rats were subjected to mesial movement by 50 g of force for 14 days under ketogenic diet. A control group was also established in which rats were subjected to the same tooth movement conditions but under a regular diet. The distance of tooth movement was measured by using precision electronic calipers. After 14 days, the rats were sacrificed and the mesial surface of the palatal root of the right first molar was analysed to determine the expression levels of IL-1β and TNF-α through immunohistochemical staining. The images obtained were then quantified using ImageJ program. <b>Results:</b> After 14 days of orthodontic tooth movement, the amount of tooth movement in the ketogenic group was lesser by approximately half the distance compared to that of the control group. Moreover, the expression of IL-1β and TNF-α was also lower. <b>Conclusions:</b> Consuming ketogenic diet reduces the expected amount of tooth movement which was evidently shown by a significant lower cytokine expression of IL-1β and TNF-α than the group under regular diet. This suggests that ketogenic diet is a contributing factor in delaying the tooth movement during orthodontic treatment.