cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.0zpc8670v
title: Improved biodiversity detection using a large-volume environmental DNA sampler with in situ filtration and implications for marine eDNA sampling strategies

description: Metabarcoding analysis of environmental DNA samples is a promising new
 tool for marine biodiversity and conservation. Typically, seawater samples
 are obtained using Niskin bottles and filtered to collect eDNA. However,
 standard sample volumes are small relative to the scale of the
 environment, conventional collection strategies are limited, and the
 filtration process is time consuming. To overcome these limitations, we
 developed a new large – volume eDNA sampler with in situ filtration,
 capable of taking up to 12 samples per deployment. We conducted three
 deployments of our sampler on the robotic vehicle Mesobot in the Flower
 Garden Banks National Marine Sanctuary in the northwestern Gulf of Mexico
 and collected samples from 20 to 400 m depth. We compared the large volume
 (~40 – 60 liters) samples collected by Mesobot with small volume (~2
 liters) samples collected using the conventional CTD rosette – mounted
 Niskin bottle approach. We sequenced the V9 region of 18S rRNA, which
 detects a broad range of invertebrate taxa, and found that while both
 methods detected biodiversity changes associated with depth, our large
 volume samples detected approximately 66% more taxa than the CTD small
 volume samples. We found that the fraction of the eDNA signal originating
 from metazoans relative to the total eDNA signal decreased with sampling
 depth, indicating that larger volume samples may be especially important
 for detecting metazoans in mesopelagic and deep ocean environments. We
 also noted substantial variability in biological replicates from both the
 large volume Mesobot and small volume CTD sample sets. Both of the sample
 sets also identified taxa that the other did not – although the number of
 unique taxa associated with the Mesobot samples was almost four times
 larger than those from the CTD samples. Large volume eDNA sampling with in
 situ filtration, particularly when coupled with robotic platforms, has
 great potential for marine biodiversity surveys, and we discuss practical
 methodological and sampling considerations for future applications.
description: The data are raw sequence files from the eDNA datasets collected
 in the manuscript (fastq.gz files). They include samples collected using
 an autonomous sampler on the robot Mesobot, and samples collected from
 Niskin bottles, as described in the manuscript. The dataset was sequenced
 over 3 runs (678A, 678B, and 678C). Six samples were resequenced due to
 suspected mis-processing (described in the manuscript appendix) (reseq-A,
 reseq-B, and reseq-C).
description: See README.docx
DOI: 10.5061/dryad.wh70rxwqm
title: Tables of microRNA and gene expression of an echolocating bat

description: MicroRNAs (miRNAs) are important post-transcriptional regulators of gene
 expression and play key roles in many biological processes, such as
 development and response to multiple stresses. However, little is known
 about their roles in generating novel phenotypes and phenotypic variation
 during the course of animal evolution. Here, we, for the first time,
 characterized the miRNAs of the cochlea in an echolocating bat
 (Rhinolophus affinis). We sampled eight individuals from two R. affinis
 subspecies with significant echolocation call frequency differences. We
 identified 365 miRNAs and 121 of them were novel. By searching sequences
 of these miRNAs precursors in multiple high-quality mammal genomes, no
 specific miRNAs were found to be shared by all echolocating mammals.
 Together with the matched mRNA-seq data, we identified 1,766
 differentially expressed genes (DEGs) between the two subspecies and 555
 of them were negatively regulated by differentially expressed miRNAs
 (DEMs). We found that almost half of known hearing genes in the list of
 all DEGs were regulated negatively by DEMs, suggesting an important role
 of miRNAs in call frequency variation of the two subspecies. These
 targeted DEGs included several important hearing genes (e.g. Piezo1,
 Piezo2 and CDH23) that have been shown to be important in ultrasonic
 hearing of echolocating mammals.
DOI: 10.5061/dryad.59zw3r29w
title: Gram reactivity of bacteria virulence factor effects for polymyxin

description: Research was to study the bacitracin effects as a polypeptide on
 prokaryote plants. The research question can bacitracin be used to
 differentiate Streptococcus pyogenes from other step bacteria with S.
 pyogenes being reactive to bacitracin and others resistant. The method
 involved using polymyxins with and without bacitracin for treatment of
 Gram-negative bacterial infections. This involved disintegration of
 bacterial cell membrane. The results showed the test differentiated and
 prevented infectants similar to the initial synopsis in plant. The
 detection of bacterial sepsis at the initial stage was challenging because
 of the nature of traits and difficulty in culturing infectiosn and lack of
 sufficient material. The polymyxins was not absorbed in the extracellular
 region of the plant. This was then supplied through the cytoplasm. It
 concluded the amount necessary for detection of Streptococcus pyogenes was
 between 0.5 μg/mL to 16 μg/mL. The polymyxins with bacitracin disrupted
 the dephosphorylaton of cell exterior of the membrane.
DOI: 10.25826/20251028-125318-0
title: Ursache-Wirkungs-Modell zum Thema Ernährungsteilhabe von Seniorinnen und Senioren in Armut - Zwischenergebnis aus dem ELSinA-Projekt
DOI: 10.5061/dryad.79cnp5hxw
title: Dataset for greenhouse gas modelling in diesel dependent communities transitioning to bioenergy

description: The data presented here are from the research article entitled “Greenhouse
 gas mitigation potential of replacing diesel fuel with wood-based
 bioenergy in an arctic Indigenous community: A pilot study in Fort
 McPherson, Canada”. Based on a pilot study realized in Northern Canada and
 life cycle assessment, we provide a set of key parameters and operational
 data gathered along the biomass supply chain to build a GHG mitigation
 scenario and compute the quantity and timing of GHG savings in the
 off-grid community of Fort McPherson, NWT. Given that GHG mitigation
 scenarios are often assessed against a relative fossil-fuel reference
 scenario, we are providing two categories of data; 1) data for the
 reference fossil fuel scenario and; 2) data along the upstream operations
 of biomass supply chains. Both categories contain data related to the
 operational processes as well as forest growth or decomposition of unused
 feedstock. Although the data presented are mostly derived from the boreal
 forest, they could help guide other communities beyond the boreal to
 develop a renewable bioenergy system and assess their GHG mitigation
 options.
description: Data for both scenarios were collected and curated from a
 community-based bioenergy project, published literature, and other GHG
 models and inventories. As many parameters as possible came from the local
 area being studied to better tailor the model to Fort McPherson’s specific
 conditions. A seven-step process was designed to guide users through
 building the biomass and fossil fuel reference scenarios, identifying the
 key parameters, and computing the quantity and timing of GHG savings.
 These steps are identified as: feedstock selection, collection and
 processing, transportation, conversion, computing GHG emissions,
 development of forest carbon dynamics, and computing forest
 carbon.
description: Bioenergy_Modelling_Dataset<br>This file contains GHG
 emissions factors, forest growth parameters, and biomass decomposition
 parameters used in the greenhouse gas (GHG) modelling performed in Buss et
 al. 2022. A metadata tab in the file describes all parameters included in
 the data file, their sources, as well as the naming convention for
 scenarios. The data file includes a total of 72 scenarios.
 Life_Cycle_Analysis_(LCA) _Script<br>This file contains the
 R code for the GHG model. The code guides users through a seven-step life
 cycle analyses designed to aid in the development of biomass and reference
 fossil fuel scenarios, and calculate the quantity and timing of GHG
 benefits. It also includes a description of model parameters and
 outputs. Model_Output_Summary_Table<br>This file
 contains a summary of a small subset of model outputs for all 72 scenarios
 included in the Bioenergy_Modelling_Data file. Model outputs included in
 this file are carbon parity times and GHG emissions at 25, 50, and 100
 years.
DOI: 10.6084/m9.figshare.31168408
title: <b>Students’ Perceptions of Decolonisation Efforts in a UK Business School</b>

description: decolonising the curriculum, business education, epistemic injustice, critical pedagogy, student voice, mixed-methods, international students, awareness
DOI: 10.6084/m9.figshare.31168408.v1
title: <b>Students’ Perceptions of Decolonisation Efforts in a UK Business School</b>

description: decolonising the curriculum, business education, epistemic injustice, critical pedagogy, student voice, mixed-methods, international students, awareness
DOI: 10.5061/dryad.djh9w0w2s
title: Review and synthesis of the global literature on domestic cat impacts on wildlife

description: A vast global literature documents that free-roaming domestic cats (Felis
 catus) have substantial negative effects on wildlife, including through
 predation, fear, disease, and competition-related impacts that have
 contributed to numerous wildlife extinctions and population declines
 worldwide. However, no study has synthesized this literature on cat
 impacts on wildlife to evaluate its overarching biases and major gaps. To
 direct future research and conservation related to cat impacts on
 wildlife, we conducted a global literature review that entailed evaluation
 and synthesis of patterns and gaps in the literature related to the
 geographic context, methods, and types of impacts studied. Our systematic
 literature search compiled 2,245 publications. We extracted information
 from 332 of these meeting inclusion criteria designed to ensure the
 relevance of studies analyzed. This synthesis of research on cat impacts
 on wildlife highlights a focus on oceanic islands, Australia, Europe, and
 North America, and on rural areas, predation, impacts of unowned cats, and
 impacts at population and species levels. Key research advances needed to
 better understand and manage cat impacts include more studies in
 underrepresented, highly biodiverse regions (Africa, Asia, South America),
 on cat impacts other than predation, and on methods designed to reduce
 impacts on wildlife. The identified areas of needed research into cat
 impacts on wildlife will be critical to further clarifying the role of
 cats in global wildlife declines and to implementing science-driven policy
 and management that benefit conservation efforts.
DOI: 10.5061/dryad.4j0zpc8dz
title: Roadside turfgrass seed mixtures: models and figures

description: Roadsides in urban areas are often seeded with turfgrass mixtures to
 provide ground cover and reduce weed abundance. Designing mixtures to
 withstand exposure to biotic and abiotic stress is challenging. Research
 from managed and natural ecosystems have shown that increasing plant
 species richness and diversity can increase groundcover and suppress weed
 cover, but it is unclear whether such relationships hold in roadside
 environments. Our objective was to determine the effect of seeded
 turfgrass species richness on ground cover and weed suppression alongside
 roadsides in diverse regions in Minnesota. We tested six turfgrass species
 in monocultures, two-way mixtures, some three-way mixtures, and a single
 six-way mixture at seven sites seeded in the fall of 2018, and seven sites
 seeded in the fall of 2019. Seeded turfgrass, weed, and bare soil coverage
 was measured at each site over two growing seasons. There was a positive
 relationship between turfgrass species richness and turfgrass cover, and
 this interaction effect increased over time. We found that increasing
 turfgrass species richness reduced bare soil coverage. Turfgrass cover was
 also more consistent across research sites (i.e., greater spatial
 stability) with increasing species richness. Our results show that
 positive relationships between plant species richness and groundcover hold
 in highly disturbed and managed roadside environments. These findings can
 improve the design of seed mixtures for roadsides and in other ecological
 contexts where vegetative cover is important.
DOI: 10.5281/zenodo.15797078
title: LSE-FS-UVigo Dataset

description: LSE-FS-UVigo is a dataset of continuous fingerspelling in Spanish Sign Language (LSE), containing 3,010 sequences from 42 signers, recorded under both studio and in-the-wild conditions. The dataset provides frame-level 3D hand keypoints (raw and preprocessed) extracted using MediaPipe, with train/validation/test splits, signer metadata, and, for the subset with RGB distribution permissions (from the LSE-Health-UVigo subset), annotated YouTube timestamps. This resource is intended to advance research in sign language recognition and benchmarking of keypoint-based recognition methods.
DOI: 10.5281/zenodo.15797079
title: LSE-FS-UVigo Dataset

description: LSE-FS-UVigo is a dataset of continuous fingerspelling in Spanish Sign Language (LSE), containing 3,010 sequences from 42 signers, recorded under both studio and in-the-wild conditions. The dataset provides frame-level 3D hand keypoints (raw and preprocessed) extracted using MediaPipe, with train/validation/test splits, signer metadata, and, for the subset with RGB distribution permissions (from the LSE-Health-UVigo subset), annotated YouTube timestamps. This resource is intended to advance research in sign language recognition and benchmarking of keypoint-based recognition methods.
DOI: 10.5061/dryad.w3r2280t7
title: Data for: Elevated inbreeding in Heliconia tortuosa is determined by tropical forest stand age, isolation, and loss of hummingbird functional diversity

description: Forest conversion and habitat loss are major threats to biological
 diversity.  Forest regeneration can mitigate the negative effects
 of old growth forest loss on species diversity, but less is known about
 the extent to which forest loss reduces genetic diversity in remnant
 populations and whether secondary forests play a role in the maintenance
 of genetic diversity. We quantified genetic diversity in a tropical
 hummingbird-pollinated understory herb, Heliconia tortuosa, across a
 landscape mosaic of primary and secondary forest regrowth. Using
 microsatellite genotypes from &gt;850 adult and juvenile plants within
 33 forest patches and extensive bird surveys, we examined the effect of
 contemporary and historical landscape features including forest age
 (primary vs. secondary forest), stand isolation, and pollinator
 assemblages on genetic diversity and levels of inbreeding in H. tortuosa.
 We found that inbreeding was up to 3x higher in secondary forest, and this
 effect was amplified with reductions in primary forest in the surrounding
 landscape through reduced observed heterozygosity in isolated fragments.
 Inbreeding in forest patches was negatively correlated with the local
 frequency of specialist long-distance foraging traplining hummingbirds.
 Traplining hummingbirds therefore appear to facilitate mating among
 unrelated plants - an inference we tested using empirically parameterized
 simulations. Higher levels of inbreeding in H. tortuosa are therefore
 associated with reduced functional diversity of hummingbirds in secondary
 forests and forest patches isolated from primary forests. Our findings
 suggest a cryptic consequence of primary forest loss and secondary forest
 regeneration through the disruption of mutualistic interactions resulting
 in the erosion of genetic diversity in a common understory plant.
DOI: 10.5061/dryad.gmsbcc2qw
title: Using marsh organs to test seed recruitment in tidal freshwater marshes

description: Premise: Seed recruitment niches along estuarine elevation gradients are
 seldom experimentally field-tested under tidal regimes of the Pacific
 Northwest of North America. Addressing this knowledge gap is important to
 better understand estuary restoration and plant community response to sea
 level rise. Methods: Germination was tested in marsh organ mesocosms
 across an elevation gradient (0.5–1.7 m above mean sea level). Seeds were
 sown on sterile peat moss, and the tops of pipes were secured with
 horticultural “frost cloth” to ensure no experimental seeds were washed
 out and no new seeds were introduced. The trials tested artificial and
 overwinter chilling regimes, as well as the presence and/or absence of a
 near-neighbor transplant. Results: Carex lyngbyei had significant
 elevation-driven germination after overwinter and artificial chilling.
 Schoenoplectus tabernaemontani had near-significant germination across
 elevation after overwinter chilling, and germination in the absence of
 competition was significantly greater than with a near-neighbor
 transplant. Discussion: Carex lyngbyei had the highest germination rate at
 higher elevations, which suggests restricted seed recruitment potential,
 and required clonal expansion to extend into lower marsh elevations.
 Identifying species-specific recruitment niches provides insight for
 restoration opportunities or invasive species monitoring, as well as for
 estuary migration under sea level rise.
description: Datasets contain temperature data automatically recorded by
 iButton data loggers (iButtonLink, LLC, Whitewater, WI, USA.
 Ibuttonlink.com), or manually observed/recorded percent seed germination
 in experimental mesocosm (marsh organ).
description: Please see ReadMe, then metadata files for each data file. R code
 is annotated with notes about analysis process, and often links to
 resources used to produce analyses.
DOI: 10.5061/dryad.ns1rn8pwb
title: Floral phenology of an Andean bellflower and pollination by Buff-tailed Sicklebill

description: The Andean bellflowers comprise an explosive radiation correlated with
 shifts to specialized pollination. One diverse clade has evolved with
 extremely curved floral tubes and are predicted to be pollinated
 exclusively by one of two parapatric species of Sicklebill hummingbirds
 (Eutoxeres). In this study we focused on the floral biology of Centropogon
 granulosus, a bellflower thought to be specialized for pollination by E.
 condamini, in a montane cloud forest site in southeastern Peru. Using
 camera traps and a pollination exclusion experiment, we documented E.
 condamini as the sole pollinator of C.granulosus. Visitation by E.
 condamini was necessary for fruit development. Flowering rates were
 unequivocally linear and conformed to the ‘steady state’ phenological
 type. Over the course of &gt;1800 hours of monitoring we recorded 12
 E. condamini visits totaling 42 seconds, indicating traplining behaviour.
 As predicted by its curved flowers, C. granulosus is exclusively
 pollinated by Buff-tailed Sicklebill within our study area. We present
 evidence for the congruence of phenology and visitation as a driver of
 specialization in this highly diverse clade of Andean bellflowers.
description: This data was collected at the Cock-of-the-Rock Lodge situated at
 ~1350 m a.s.l. in the Kosñipata Valley, Department of Cusco, Peru
 (-13.055, -71.548 DD). Data in the 'raw_data' folder is not
 processed, and was used as-is in the analysis. Please refer to the
 accompanying publication for a detailed description of the methods used to
 collect this data.
description: This dataset was analysed via R scripts in the accompanying
 Zenodo repository. To run the .R files, we recommend first opening the
 .RProj file in RStudio and installing the package `here`. This will allow
 you to run all of the .R scripts without changing any of the working
 directories.
DOI: 10.5061/dryad.g4f4qrfsr
title: Precipitation Efficiency Constraint on Climate Change

description: Precipitation efficiency (PE) relates cloud condensation to precipitation
 and intrinsically binds atmospheric circulation to the hydrological cycle.
 Due to PE’s inherent microphysical dependencies, definitions and estimates
 vary immensely. Consequently, PE’s sensitivity to greenhouse warming and
 implications for climate change are poorly understood. Here, we quantify
 PE’s role in climate change by defining a simple index as the ratio of
 surface precipitation to condensed water path. This macroscopic metric is
 reconcilable with microphysical PE measures and higher is associated with
 stronger mean Walker circulation. We further find that state-of-the-art
 climate models disagree on the sign and magnitude of
 future changes. This sign disagreement originates from models’
 convective parameterizations. Critically, models with increasing under
 greenhouse warming, in line with cloud-resolving simulations, show greater
 slowdown of the large-scale Hadley and Walker circulations and a two-fold
 greater increase in extreme rainfall than models with decreasing.
DOI: 10.5061/dryad.v15dv41z6
title: Treatment of bacterial infestation for polymyxin

description: Research was to study polymyxin supplied for treatment pathogens
 infestation and sepsis in plants. The research question was can this be
 used for Gram positive instead of Gram negative infestations. The method
 involved examination of the septic cytoplasm from the interior or
 extracellular cause. This involved determination of more than a particular
 membrane of bacteria. The results showed the infection was by bacteria and
 less frequent fungi or parasites. The infestation began at some other
 location and the body and transferred through the cytoplasm to the
 extracellular membrane. Polymyxin sulfate was composed of two doses. This
 altered the bacterial cell structure and causes loss out of cellular
 contents. It concluded the sepsis was plant cells response to infection.
 These developed within 3 to 7 days after exposure to the bacteria.