DOI: 10.5061/dryad.12jm63z71
title: Trophy constrains the temperature effect on ciliate species turnover rates

description: We applied FlowCam analysis and cross-validated by 18S rDNA-sequences and
 taxonomic literature to study seasonal and short-term population dynamics
 and species turnover in ciliate plankton during 15 months with
 high-frequency samplings in a shallow temperate estuary in Denmark.
 FlowCam imagery identified 27 phenotypic ciliate entities, and eight
 genotypes were identified. The analyses showed strong seasonality in
 biomass, abundance, diversity, trophy, and species turnover. The
 mixotrophic ciliates increased 30-fold from winter to summer, whereas the
 abundance of heterotrophic ciliates increased only five times. The two
 trophic groups also displayed contrasting seasonal diversity patterns.
 Heterotrophic ciliates increased in richness from 5.5 to 10.5 species in
 10 mL, whereas species richness of the mixotrophic community, dominated by
 Mesodinium rubrum, remained relatively constant at three species per 10 mL
 sample. Daily species turnover calculated from the decay of similarity was
 highest for heterotrophic ciliates, and community change rates of 3.1,
 15.8 and 30.6 % d-1 were related to ambient temperatures of 4.8, 8.4 and
 16 °C. Oscillating species-specific growth rates due to prey-predator
 interactions can explain faster species turnover rates in heterotrophic
 ciliates. In contrast, the mixotrophic ciliates community harvest a common
 supplementary energy source, which may dampen their species-specific
 population oscillations.
description: # Trophy constrains the temperature effect on ciliate species turnover
 rates Dataset DOI: [10.5061/dryad.12jm63z71](10.5061/dryad.12jm63z71) ##
 Description of the data and file structure Data for ciliate diversity was
 collected at the inner part of RF in Denmark (55°41'30.19"N,
 12°4'55.24"E). seasonal and short-term population dynamics and
 species turnover in ciliate plankton during 15 months with high-frequency
 samplings in a shallow temperate estuary in Denmark. Contact Hans H
 Jakobsen ([hhja@ecos.au.dk](mailto:hhja@ecos.au.dk)) with any questions.
 This manuscript has been accepted for publication (02/14/2025): Hansen
 J.L.S., Altenburger A., Haraguchi L., Carstensen J. Jakobsen H.H.
 "Trophy constrains the temperature effect on ciliate species turnover
 rates" ECOSPHERE (accepted). 10.1111/oik.10914 Data files include:
 ### Files and variables #### File: data\_for\_sharing.xlsx ###
 data_for_sharing.xlsx @SHEET"pier" This sheet contains data on
 temperature and salinity. \| variabl           | units                   
 | description                                                             
                     | \| :------------------ | :--------------------: |
 :----------------------------------------------------------------------------------| \| doy                   | number             | Gregorian day of year, january 1st = DOY 1.                         | \| temp                | degree Celsius | temperature in degree Celsius                                                | \| Salinity            | salinity                | salinity measured in parts per thousands(‰)                      | ### data_for_sharing.xlsx @SHEET"ST60" This sheet contains data on dissolved totel Nitrogen \| variables | units     | description                                                                                                        | \| :---------- | :----------: | :---------------------------------------------------------------------------------------------------- | \| doy        | number | Gregorian day of year, january 1st = DOY 1.                                                | \| DIN        | µM         | total dissolved Nitrogen in micro mol concentration                                | ### data_for_sharing.xlsx @SHEET"Ciliates" This sheeet contain metric data derived from individual FlowCam images \| variables | units     | description                                                                                                          | \| :---------- | :-----------: | :----------------------------------------------------------------------------------------------------- | \| date       | date        | Gregorian date                                                                                                   | \| year       | number  | Gregorian year                                                                                                   | \| month    | number | Gregorian month                                                                                               | \| day          | number | Gregorian day                                                                                                     | \| doy          | number | Gregorian day of year, january 1st = DOY 1.                                                 | \| ESD          | µm         | Equivalent diameter of individual cells                                                          | \| volumen | µm3       | Biovolumen of individual cells                                                                         | \| pgC         | PgC        | picogram carbon cell-1                                                                                      | ### data\_for\_sharing.xlsx @SHEET"Wind" This sheeet contain climate wind data derived from individual FlowCam images \| variables                               | units                | description                                                                       | \| :------------------------------------ | :------------------: | :-------------------------------------------------------------------------| \| date                                       | date                 | Gregorian date | \| Average daily wind speed | meters sec-1 | average daily wind speed                                             | \| Daily wind direction (deg.) | grader           | average daily wind direction                                        | \| Pressure                               | millibar           | atmospheric air pressure                                             | \| doy                                        | number           | Gregorian day of year, january 1st = DOY 1.            |