cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14328618.v1
title: Yield, market quality, and leaf spots partial resistance of interspecific peanut progenies

description: Development of leaf spots resistant runner peanut cultivars is one of the major demands in Brazil. In the 2009/10 crop season, 12 pre-breeding progenies (LPM) were selected out of 380 plants of an interspecific BC1F3 population for leaf spots resistance and agronomic traits. In the 2010/11, the 12 LPMs were evaluated for leaf spots resistance, without fungicide sprays. The recurrent parent (susceptible control) and all LPMs were susceptible; partial resistant segregating plants were selected. Some progenies had yield, pod and seed shape similar to the control. The 12, and the 5 LPMs selected in the 2011/12 and 2012/13 crop seasons, respectively, were evaluated under appropriate disease management. Differences were detected for pod yield and weight of 100 seeds. Seed/pod weight ratio, oil content and oleic/linoleic ratio were similar to thecontrol. Individual plants were selected for pod yield and market quality. Variability detected in the first backcross generation for partial resistance and agronomic traits will be useful.
DOI: 10.6084/m9.figshare.14328619
title: Genetic progress and potential of common bean families obtained by recurrent selection

description: Abstract The objective of this study was to estimate the genetic gain of two recurrent selection cycles in common bean breeding and identify families with the potential to generate superior lines. The base population, cycle zero (C0), was obtained by combining 20 carioca bean parents, populations with favorable phenotypes for several agronomically important traits. The parents were recombined in a circulant diallel scheme, in which each parent participated in two crosses, generating 20 populations. From these populations, families were derived and evaluated for three seasons in the generations F2:3, F2:4 and F2:5. The same procedures of recombination and evaluation in C0 were performed in cycle one (CI). The genetic gain for yield, estimated from the simultaneous evaluation of the 40 best families of each cycle, was 8.6%. Families with potential to generate superior lines to cultivar Pérola were identified, especially among the CI families.
DOI: 10.6084/m9.figshare.14328619.v1
title: Genetic progress and potential of common bean families obtained by recurrent selection

description: Abstract The objective of this study was to estimate the genetic gain of two recurrent selection cycles in common bean breeding and identify families with the potential to generate superior lines. The base population, cycle zero (C0), was obtained by combining 20 carioca bean parents, populations with favorable phenotypes for several agronomically important traits. The parents were recombined in a circulant diallel scheme, in which each parent participated in two crosses, generating 20 populations. From these populations, families were derived and evaluated for three seasons in the generations F2:3, F2:4 and F2:5. The same procedures of recombination and evaluation in C0 were performed in cycle one (CI). The genetic gain for yield, estimated from the simultaneous evaluation of the 40 best families of each cycle, was 8.6%. Families with potential to generate superior lines to cultivar Pérola were identified, especially among the CI families.
DOI: 10.6084/m9.figshare.14328622.v1
title: IPR AFRODITE - new oat cultivar with nematode resistance

description: Abstract The oat cultivar IPR Afrodite, resulting from a cross between CFT 2 and the inbred line ER 88144-1, is high-yielding, has grain quality traits that are relevant for food processing and industry, and is resistant to the root-knot nematode species Meloidogyne incognita, M. javanica, M. paranaensis and M. enterolobii.
DOI: 10.6084/m9.figshare.14328622
title: IPR AFRODITE - new oat cultivar with nematode resistance

description: Abstract The oat cultivar IPR Afrodite, resulting from a cross between CFT 2 and the inbred line ER 88144-1, is high-yielding, has grain quality traits that are relevant for food processing and industry, and is resistant to the root-knot nematode species Meloidogyne incognita, M. javanica, M. paranaensis and M. enterolobii.
DOI: 10.6084/m9.figshare.14328624
title: Genetics and identification of markers linked to multiflorous spikelet in hexaploid oat

description: Abstract The formation of naked grains is directly associated with the formation of multiflorous spikelets in oats. The objectives of this study were to determine the genetics of multiflorous spikelet and to identify molecular markers linked to this character in hexaploid oat. Genetic analysis for multiflorous spikelet was performed in the F5 and F6 generations of two oat populations. DNA extracted from F5:6 plants were assayed with 6,000 genome-wide single nucleotide polymorphism (SNP) markers using a genotyping platform developed for oat. Genetic analysis indicated the presence of a major gene controlling multiflorous spikelet in the UFRGS 01B7114-1-3 x UFRGS 006013-1 population. The SNP marker GMI_ES17_c5923_221 showed strong association with the multiflorous spikelet phenotype. These results suggest that the marker GMI_ES17_c5923_221 should be linked to a gene controlling multiflorous spikelet in the oat lines evaluated in this study.
DOI: 10.6084/m9.figshare.14328624.v1
title: Genetics and identification of markers linked to multiflorous spikelet in hexaploid oat

description: Abstract The formation of naked grains is directly associated with the formation of multiflorous spikelets in oats. The objectives of this study were to determine the genetics of multiflorous spikelet and to identify molecular markers linked to this character in hexaploid oat. Genetic analysis for multiflorous spikelet was performed in the F5 and F6 generations of two oat populations. DNA extracted from F5:6 plants were assayed with 6,000 genome-wide single nucleotide polymorphism (SNP) markers using a genotyping platform developed for oat. Genetic analysis indicated the presence of a major gene controlling multiflorous spikelet in the UFRGS 01B7114-1-3 x UFRGS 006013-1 population. The SNP marker GMI_ES17_c5923_221 showed strong association with the multiflorous spikelet phenotype. These results suggest that the marker GMI_ES17_c5923_221 should be linked to a gene controlling multiflorous spikelet in the oat lines evaluated in this study.
DOI: 10.6084/m9.figshare.14328627.v1
title: Evaluation of SRAP markers for mapping of Pisum sativum L.

description: Abstract Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 were generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.
DOI: 10.6084/m9.figshare.14328627
title: Evaluation of SRAP markers for mapping of Pisum sativum L.

description: Abstract Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 were generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.
DOI: 10.6084/m9.figshare.14328628
title: Missing value imputation in multi-environment trials: Reconsidering the Krzanowski method

description: Abstract We propose a new methodology for multiple imputation when faced with missing data in multi-environmental trials with genotype-by-environment interaction, based on the imputation system developed by Krzanowski that uses the singular value decomposition (SVD) of a matrix. Several different iterative variants are described; differential weights can also be included in each variant to represent the influence of different components of SVD in the imputation process. The methods are compared through a simulation study based on three real data matrices that have values deleted randomly at different percentages, using as measure of overall accuracy a combination of the variance between imputations and their mean square deviations relative to the deleted values. The best results are shown by two of the iterative schemes that use weights belonging to the interval [0.75, 1]. These schemes provide imputations that have higher quality when compared with other multiple imputation methods based on the Krzanowski method.
DOI: 10.6084/m9.figshare.14328628.v1
title: Missing value imputation in multi-environment trials: Reconsidering the Krzanowski method

description: Abstract We propose a new methodology for multiple imputation when faced with missing data in multi-environmental trials with genotype-by-environment interaction, based on the imputation system developed by Krzanowski that uses the singular value decomposition (SVD) of a matrix. Several different iterative variants are described; differential weights can also be included in each variant to represent the influence of different components of SVD in the imputation process. The methods are compared through a simulation study based on three real data matrices that have values deleted randomly at different percentages, using as measure of overall accuracy a combination of the variance between imputations and their mean square deviations relative to the deleted values. The best results are shown by two of the iterative schemes that use weights belonging to the interval [0.75, 1]. These schemes provide imputations that have higher quality when compared with other multiple imputation methods based on the Krzanowski method.
DOI: 10.6084/m9.figshare.14328631
title: Tomato second cycle hybrids as a source of genetic variability for fruit quality traits

description: Abstract The objective of this study was to investigate the phenotypic and molecular variability in a F2 generation derived from a SCH (Second Cycle Hybrid) in order to detect QTLs for some fruit traits of tomato. Genome coverage at different levels was achieved by three types of molecular markers (polypeptides, sequence-related amplified polymorphism-SRAP and amplified restriction fragment polymorphism - AFLP). Different degrees of polymorphism were detected by SRAP and AFLP at the DNA structure level and also by polypeptides at the DNA expression level. The first two markers, associated with phenotypic variation, detected QTLs involved in important agronomic traits such as fruit shelf life, soluble solids content, pH, and titratable acidity. New gene blocks originated by recombination during the first cycle of crossing were detected. This study confirmed that the observed phenotypic differences represent a new gene rearrangement and that these new gene blocks are responsible for the presence of the genetic variability detected for these traits.
DOI: 10.6084/m9.figshare.14328631.v1
title: Tomato second cycle hybrids as a source of genetic variability for fruit quality traits

description: Abstract The objective of this study was to investigate the phenotypic and molecular variability in a F2 generation derived from a SCH (Second Cycle Hybrid) in order to detect QTLs for some fruit traits of tomato. Genome coverage at different levels was achieved by three types of molecular markers (polypeptides, sequence-related amplified polymorphism-SRAP and amplified restriction fragment polymorphism - AFLP). Different degrees of polymorphism were detected by SRAP and AFLP at the DNA structure level and also by polypeptides at the DNA expression level. The first two markers, associated with phenotypic variation, detected QTLs involved in important agronomic traits such as fruit shelf life, soluble solids content, pH, and titratable acidity. New gene blocks originated by recombination during the first cycle of crossing were detected. This study confirmed that the observed phenotypic differences represent a new gene rearrangement and that these new gene blocks are responsible for the presence of the genetic variability detected for these traits.
DOI: 10.6084/m9.figshare.14328634
title: Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum

description: Abstract Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index () ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.
DOI: 10.6084/m9.figshare.14328634.v1
title: Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum

description: Abstract Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index () ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.
DOI: 10.6084/m9.figshare.14328633
title: Genetic parameters, adaptability and stability to selection of yellow passion fruit hybrids

description: Abstract The objective of this study was to evaluate the stability and adaptability, using the method of harmonic means of the relative performance of genetic values and to estimate the genetic components of variance and average via mixed models of 14 genotypes of passion fruit in three environments. Data were obtained in a random block design with three replicates and nine plants per plot. For the hybrids in the final validation phase, the estimates of heritability and genetic gains in the evaluated environments showed good prospects for selection of superior genotypes. There was a pronounced effect of genotype-environment interaction (GxE) for all traits investigated except fruit length, percentage of pulp, soluble solids, titratable acidity and SS/TA ratio. The most stable and adaptable hybrids in the evaluated environments were BRS Gigante Amarelo, HFOP-09, H09-09, GP09-02, GP09-03 and BRS Sol do Cerrado.
DOI: 10.6084/m9.figshare.14328633.v1
title: Genetic parameters, adaptability and stability to selection of yellow passion fruit hybrids

description: Abstract The objective of this study was to evaluate the stability and adaptability, using the method of harmonic means of the relative performance of genetic values and to estimate the genetic components of variance and average via mixed models of 14 genotypes of passion fruit in three environments. Data were obtained in a random block design with three replicates and nine plants per plot. For the hybrids in the final validation phase, the estimates of heritability and genetic gains in the evaluated environments showed good prospects for selection of superior genotypes. There was a pronounced effect of genotype-environment interaction (GxE) for all traits investigated except fruit length, percentage of pulp, soluble solids, titratable acidity and SS/TA ratio. The most stable and adaptable hybrids in the evaluated environments were BRS Gigante Amarelo, HFOP-09, H09-09, GP09-02, GP09-03 and BRS Sol do Cerrado.
DOI: 10.6084/m9.figshare.14328636
title: Adjusting the Scott-Knott cluster analyses for unbalanced designs

description: Abstract The Scott-Knott cluster analysis is an alternative approach to mean comparisons with high power and no subset overlapping. It is well suited for the statistical challenges in agronomy associated with testing new cultivars, crop treatments, or methods. The original Scott-Knott test was developed to be used under balanced designs; therefore, the loss of a single plot can significantly increase the rate of type I error. In order to avoid type I error inflation from missing plots, we propose an adjustment that maintains power similar to the original test while adding error protection. The proposed adjustment was validated from more than 40 million simulated experiments following the Monte Carlo method. The results indicate a minimal loss of power with a satisfactory type I error control, while keeping the features of the original procedure. A user-friendly SAS macro is provided for this analysis.
DOI: 10.6084/m9.figshare.14328636.v1
title: Adjusting the Scott-Knott cluster analyses for unbalanced designs

description: Abstract The Scott-Knott cluster analysis is an alternative approach to mean comparisons with high power and no subset overlapping. It is well suited for the statistical challenges in agronomy associated with testing new cultivars, crop treatments, or methods. The original Scott-Knott test was developed to be used under balanced designs; therefore, the loss of a single plot can significantly increase the rate of type I error. In order to avoid type I error inflation from missing plots, we propose an adjustment that maintains power similar to the original test while adding error protection. The proposed adjustment was validated from more than 40 million simulated experiments following the Monte Carlo method. The results indicate a minimal loss of power with a satisfactory type I error control, while keeping the features of the original procedure. A user-friendly SAS macro is provided for this analysis.
DOI: 10.6084/m9.figshare.14328637
title: Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction

description: Abstract Cenchrus ciliaris L. is an apomictic forage grass grown in pastures and rangelands of the semi-arid tropics. It reproduces predominantly through apomixis; rarely, obligate sexual plants have also been reported. Absence of sexual reproduction limits the possibility of genetic improvement through hybridization. This study reports on hybridization of an obligate sexual, self-incompatible buffelgrass with pollen from apomictic plants towards development of an F2 population segregating for mode of reproduction and use of sequence characterized amplified region (SCAR) markers for screening the population. The segregation ratio of 3:1 (facultative: apomictic) was observed in the F1 generation, whereas it was 1:2:1 (apomictic: facultative: sexual) in the F2 generation. A number of obligate sexual F2 progenies with desirable agronomic traits were obtained. The SCAR markers were able to screen out apomictic plants from sexual ones, but failed to discriminate between facultative and sexual. Marker-assisted screening could be useful for introgression of desirable trait(s) in the apomictic genotype through hybridization.
DOI: 10.6084/m9.figshare.14328637.v1
title: Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction

description: Abstract Cenchrus ciliaris L. is an apomictic forage grass grown in pastures and rangelands of the semi-arid tropics. It reproduces predominantly through apomixis; rarely, obligate sexual plants have also been reported. Absence of sexual reproduction limits the possibility of genetic improvement through hybridization. This study reports on hybridization of an obligate sexual, self-incompatible buffelgrass with pollen from apomictic plants towards development of an F2 population segregating for mode of reproduction and use of sequence characterized amplified region (SCAR) markers for screening the population. The segregation ratio of 3:1 (facultative: apomictic) was observed in the F1 generation, whereas it was 1:2:1 (apomictic: facultative: sexual) in the F2 generation. A number of obligate sexual F2 progenies with desirable agronomic traits were obtained. The SCAR markers were able to screen out apomictic plants from sexual ones, but failed to discriminate between facultative and sexual. Marker-assisted screening could be useful for introgression of desirable trait(s) in the apomictic genotype through hybridization.
DOI: 10.6084/m9.figshare.14328640
title: BRS Kurumi and BRS Capiaçu - New elephant grass cultivars for grazing and cut-and-carry system

description: Abstract Cultivar BRS Kurumi is characterized by short plant height, short internodes, high dry matter production (29.25 t ha-1yr-1), has a high nutritional value and is recommended for the cut-and-carry system or grazing. Cultivar BRS Capiaçu, indicated for silage and the cut-and-carry system, has tall upright growth, high dry matter production (49.75 t ha-1yr-1), good forage quality, is lodging-resistant and suitable for mechanical harvesting. Both cultivars are vegetatively propagated by stem cuttings.
DOI: 10.6084/m9.figshare.14328640.v1
title: BRS Kurumi and BRS Capiaçu - New elephant grass cultivars for grazing and cut-and-carry system

description: Abstract Cultivar BRS Kurumi is characterized by short plant height, short internodes, high dry matter production (29.25 t ha-1yr-1), has a high nutritional value and is recommended for the cut-and-carry system or grazing. Cultivar BRS Capiaçu, indicated for silage and the cut-and-carry system, has tall upright growth, high dry matter production (49.75 t ha-1yr-1), good forage quality, is lodging-resistant and suitable for mechanical harvesting. Both cultivars are vegetatively propagated by stem cuttings.