cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/gm2f3-3s805
title: bl21_1-1-1_sq1_cell1

description: BL21_1-1-1 - SQ1 - IT1s [added 2019-03-06 11:35:47] IT1s
description: <strong>Tilt Series Date:</strong> 2019-02-22</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK1, BL21 dHisdHA RQABpCDF+EFGpRSF S2-ZC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 87.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Petya Krasteva</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 3mL culture started from glycerol stock. TB + salts + Kan (40ug/mL) + Strepto (70ug/mL) incubated at 37C - 220rpm until OD 1.5 (overdue a little bit). IPTG added and incubated at 17C-ON.OD at 3.4 before freezing the next day.</p>
<strong>Sample Preparation:</strong> Diluted to OD 1 - Broad 100% 22C BT4s BF0 - front blot only</p>
  wnj2019-02-22-5_slicer19974.jpg: Rings in the periplasmic space wnj2019-02-22-5_slicer19975.jpg: Teeth seen multiple times
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-02-22-5/rawdata/</p>bl21_1-1-1_sq1_cell1_orig.st,  Tilt Series (Pixel Size 0.54 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-02-22-5/rawdata/bl21_1-1-1_sq1_cell1_orig.st"
        > <i class="download icon"></i> Download </a></p>

        bl21_1-1-1_sq1_cell1.rec,  Reconstruction (Pixel Size 0.54 nm), 175.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-02-22-5/3dimage_101702/bl21_1-1-1_sq1_cell1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5517/ccdc.csd.cc2nh6ft
title: CCDC 2457476: Experimental Crystal Structure Determination

description: Related Article: Long-Kun Chen, Yi-Mai Yin, Qin Luo, Jia-Qi Shao, Cong-Hai Zhang, Sheng-Jiao Yan|2025|J.Org.Chem.|90|11344|doi:10.1021/acs.joc.5c01408
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2nh6gv
title: CCDC 2457477: Experimental Crystal Structure Determination

description: Related Article: Long-Kun Chen, Yi-Mai Yin, Qin Luo, Jia-Qi Shao, Cong-Hai Zhang, Sheng-Jiao Yan|2025|J.Org.Chem.|90|11344|doi:10.1021/acs.joc.5c01408
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.22002/xgraa-t4908
title: P_aeruginosa_PA14_DfliF_Krios

description: Raw data files of P_aeruginosa_PA14_DfliF_Krios
description: <strong>Tilt Series Date:</strong> 2018-09-29</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (50.0°, -50.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> I (mohammed) obtained the cells from Shannon Park (Dianne Newman lab). The cells are available in their library of Pseudomonas aeruginosa PA14.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated one colony from an LB agar plate and grew the cells overnight in LB with 100 ug/ml of Gentamycin. Then I concentrated the cells three times and mixed them with gold nanoparticles and added 3 ul on to thick carbon R2 grids and blotted them and plunge froze them (force either 6 or 3, time either 3 or 2 seconds respectively, drain time 1 second).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-37/rawdata/</p>Pseudomonas_DfliF36.mrc,  Tilt Series (Pixel Size 0.53 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-37/rawdata/Pseudomonas_DfliF36.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudomonas_DfliF36_full.rec,  Reconstruction (Pixel Size 2.12 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-37/3dimage_97211/Pseudomonas_DfliF36_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5517/ccdc.csd.cc2nqg84
title: CCDC 2464446: Experimental Crystal Structure Determination

description: Related Article: Ghodrat Mahmoudi, Isabel Garcia-Santos, Alfonso Castiñeiras, Asmet N. Azizova, Elizaveta V. Panov, Ömer Faruk Tutar, Damir A. Safin|2025|J.Mol.Struct.|1349|143483|doi:10.1016/j.molstruc.2025.143483
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2nqjdb
title: CCDC 2464512: Experimental Crystal Structure Determination

description: Related Article: Joseph M. Parr, Cameron Jones|2025|Organometallics|44|1644|doi:10.1021/acs.organomet.5c00223
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2nqjfc
title: CCDC 2464513: Experimental Crystal Structure Determination

description: Related Article: Joseph M. Parr, Cameron Jones|2025|Organometallics|44|1644|doi:10.1021/acs.organomet.5c00223
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.22002/anf2v-fkw82
title: JV9082_K3_janelia

description: JV9082:DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2019-12-02</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -2.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-38/rawdata/</p>9082_Box1_No1_S138.mrc,  Tilt Series (Pixel Size 0.261 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-38/rawdata/9082_Box1_No1_S138.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_Box1_No1_S138_full.rec,  Reconstruction (Pixel Size 1.044 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-02-38/3dimage_112543/9082_Box1_No1_S138_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5517/ccdc.csd.cc2nrjhg
title: CCDC 2465476: Experimental Crystal Structure Determination

description: Related Article: Li-Xin You, Xin-Yu Wang, Jin-Rong Li, Jie Guo, Gang Xiong, Fu ding, Ya-Guang Sun|2025|CrystEngComm|27|5625|doi:10.1039/D5CE00627A
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc209k41
title: CCDC 1856222: Experimental Crystal Structure Determination

description: Related Article: Brian R. Nichols, Jeffrey L. Petersen, Brian S. Dolinar, Brian V. Popp|2025|New J.Chem.|49|14073|doi:10.1039/D5NJ01429H
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.22002/v40ga-am894
title: Ecoli_EPEC_8612

description: These are E coli cells (EPEC 8612) obtained from Ritesh (Ilan Rosenshine lab at the Hebrew  .  university). The idea is to image bacterial nanotubes in these cells.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> These cells were prepared with Ritesh in Ilan Rosenshine lab at the Hebrew University. </p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. Then washed with PBS and blotted and then plunge . frozen. Yuxi helped in sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-24/rawdata/</p>EPEC_8612_tilt17.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-24/rawdata/EPEC_8612_tilt17.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_tilt17.mrc.mdoc,  19.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-24/rawdata/EPEC_8612_tilt17.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5517/ccdc.csd.cc209k63
title: CCDC 1856224: Experimental Crystal Structure Determination

description: Related Article: Brian R. Nichols, Jeffrey L. Petersen, Brian S. Dolinar, Brian V. Popp|2025|New J.Chem.|49|14073|doi:10.1039/D5NJ01429H
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2mhrnk
title: CCDC 2428219: Experimental Crystal Structure Determination

description: Related Article: Zheng Liu, Jun-Jie Fang, Zhi-Yi Wang, Yun-Peng Xie, Xing Lu|2025|Chem.Commun.|61|12781|doi:10.1039/D5CC01663K
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2mhrsp
title: CCDC 2428223: Experimental Crystal Structure Determination

description: Related Article: Zheng Liu, Jun-Jie Fang, Zhi-Yi Wang, Yun-Peng Xie, Xing Lu|2025|Chem.Commun.|61|12781|doi:10.1039/D5CC01663K
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2nsnmr
title: CCDC 2466565: Experimental Crystal Structure Determination

description: Related Article: Lennart Stoess, Lutz Greb|2025|Chemical Science|16|15387|doi:10.1039/D5SC03200H
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2ntgcb
title: CCDC 2467332: Experimental Crystal Structure Determination

description: Related Article: Xiaorong Jin, Min Wang, Yutao Rao, Mingbo Zhou, Bangshao Yin, Ling Xu, Tomohiro Higashino, Atsuhiro Osuka, Jianxin Song|2025|Org.Lett.|27|8690|doi:10.1021/acs.orglett.5c02623
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2ntgdc
title: CCDC 2467333: Experimental Crystal Structure Determination

description: Related Article: Xiaorong Jin, Min Wang, Yutao Rao, Mingbo Zhou, Bangshao Yin, Ling Xu, Tomohiro Higashino, Atsuhiro Osuka, Jianxin Song|2025|Org.Lett.|27|8690|doi:10.1021/acs.orglett.5c02623
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.5517/ccdc.csd.cc2ntgfd
title: CCDC 2467334: Experimental Crystal Structure Determination

description: Related Article: Xiaorong Jin, Min Wang, Yutao Rao, Mingbo Zhou, Bangshao Yin, Ling Xu, Tomohiro Higashino, Atsuhiro Osuka, Jianxin Song|2025|Org.Lett.|27|8690|doi:10.1021/acs.orglett.5c02623
description: An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
DOI: 10.22002/haszw-7z446
title: Shewanella oneidensis MR-1

description: Raw data files of Shewanella oneidensis MR-1
description: <strong>Tilt Series Date:</strong> 2018-05-27</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I got these cells from Poorna (which she got from Mow's group in USC). I imaged them in the same time I imaged the dFlgH mutant to make sure that under these conditions the wild type cells will still express the PL-subcomplexes.</p>
<strong>Sample Preparation:</strong> Cells were grown in LB overnight at 30 C. The idea was to compare them with dFlgH cells which were grown under the same conditions to make sure that the WT cells express the PL-subcomplexes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-77/rawdata/</p>tilt115.mrc,  Tilt Series (Pixel Size 0.57 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-77/rawdata/tilt115.mrc"
        > <i class="download icon"></i> Download </a></p>

        tilt115_full.rec,  Reconstruction (Pixel Size 2.28 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-05-27-77/3dimage_93569/tilt115_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w5t0c-v9846
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-152/rawdata/</p>JV9082_201808290164.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-152/rawdata/JV9082_201808290164.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_201808290164_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-152/3dimage_96677/JV9082_201808290164_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.8kv249
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Spectrolebias inaequipinnatus)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0008154-250827131500795/datasets/export for details.
DOI: 10.22002/b950k-s6074
title: mini cells

description: Mini cells (FtsA FtsZ over-expression)
description: <strong>Tilt Series Date:</strong> 2019-11-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Craig Roy lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> FtsA FtsZ over-expression induced by 0.1mM IPTG in liquid culture. O.D. reached 1.8 after ~40 hours. </p>
<strong>Sample Preparation:</strong> Concentrate 600uL of O.D. 1.8 cells, collect supernatant from 500Xg 2min spin, then concentrate again w/ 1000xg 5min spin. Resuspend pellet to 50uL as stock. Vitrobot set to 22 C, 100% humidity. Apply 4uL of 20X diluted stock, blot manually. Quantifoil R2/2 copper.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-15-19/rawdata/</p>20191115_Lpmini_87.mrc,  Tilt Series (Pixel Size 0.1339 nm), 7.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-15-19/rawdata/20191115_Lpmini_87.mrc"
        > <i class="download icon"></i> Download </a></p>

        20191115_Lpmini_87_part37_1.rec,  Reconstruction (Pixel Size 1.0712 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-15-19/3dimage_108581/20191115_Lpmini_87_part37_1.rec"
        > <i class="download icon"></i> Download </a></p>