DOI: 10.5061/dryad.dfn2z358b
title: Data from: Green derivative synchronous spectrofluorimetry for kinetic stability study of florfenicol

description: This dataset contains data representing synchronous fluorescence spectra
 obtained upon utilizing spectrofluorometry as a highly sensitive analysis
 tool for the simultaneous determination of Florfenicol with its different
 degradation products obtained after stress testing. Synchronous
 fluorimetry was used followed by applying either first or second
 derivatization according to the type of degradation aiming to obtain
 highly resoluted spectra. Different degradation pathways according to
 published literature were used to interpret degradation kinetic
 parameters, which were in accordance with previously reported articles.
 The dataset also includes different validation parameters applied.
description: The dataset was collected during the measurement of fluorescence
 following the acidic, alkaline, oxidative, and photolytic degradation of
 FLR at different temperature settings at predetermined time intervals.
 Labsolution software was used to apply synchronous fluorimetry at
 different Δλ, followed by either first or second derivatization. The
 experiments were carried out in the Central Laboratory at the Faculty of
 Pharmacy Delta University for Science and Technology. The synchronous
 fluorescence spectra illustrated in the following table are the result of
 measuring the synchronous fluorescence relative intensity on the Y axis
 versus the emission wavelength on the X axis.
description: # Synchronous fluorescence spectra for stress testing of florfenicol
 [https://doi.org/10.5061/dryad.dfn2z358b](https://doi.org/10.5061/dryad.dfn2z358b) ## Description of the data and file structure | **F1** | Scanning of 10.0 µg/mL at ∆ƛ=20 nm | | ------- | --------------------------------------------------------------------------------------------------------- | | **F2** | Scanning of 10.0 µg/mL at ∆ƛ=40 nm | | **F3** | Scanning of 10.0 µg/mL at ∆ƛ=60 nm | | **F4** | Scanning of 10.0 µg/mL at ∆ƛ=80 nm | | **F5** | Scanning of 10.0 µg/mL at ∆ƛ=100 nm | | **F6** | Scanning of 10.0 µg/mL at ∆ƛ=120 nm | | **F7** | 0.5µg/mL FLR | | **F8** | 2.0 µg/mL FLR | | **F9** | 4.0 µg/mL FLR | | **F10** | 6.0 µg/mL FLR | | **F11** | 12.0 µg/mL FLR | | **F12** | 16.0 µg/mL FLR | | **F13** | Alkaline degradation of 10.0 µg/mL FLR using 1M NaOH at 100̊C at ∆ƛ=20 | | **F14** | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 40 using 1M NaOH at 100̊C | | **F15** | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 60 using 1M NaOH at 100̊C | | **F16** | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 80 using 1M NaOH at 100̊C | | **F17** | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 100 using 1M NaOH at 100̊C | | **F18** | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 120 using 1M NaOH at 100̊C | | **F19** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 5 minutes using 1M NaOH | | **F20** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 10 minutes using 1M NaOH | | **F21** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 20 minutes using 1M NaOH | | **F22** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 25 minutes using 1M NaOH | | **F23** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 30 minutes using 1M NaOH | | **F24** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 45 minutes using 1M NaOH | | **F25** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 60 minutes using 1M NaOH | | **F26** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 5 minutes using 1M NaOH | | **F27** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 10 minutes using 1M NaOH | | **F28** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 15 minutes using 1M NaOH | | **F29** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 20 minutes using 1M NaOH | | **F30** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 25 minutes using 1M NaOH | | **F31** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 30 minutes using 1M NaOH | | **F32** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 45 minutes using 1M NaOH | | **F33** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 60 minutes using 1M NaOH | | **F34** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 90 minutes using 1M NaOH | | **F35** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 5 minutes using 1M NaOH | | **F36** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 10 minutes using 1M NaOH | | **F37** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 20 minutes using 1M NaOH | | **F38** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 25 minutes using 1M NaOH | | **F39** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 30 minutes using 1M NaOH | | **F40** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 45 minutes using 1M NaOH | | **F41** | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 60 minutes using 1M NaOH | | **F42** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 5 minutes using 1M NaOH | | **F43** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 15 minutes using 1M NaOH | | **F44** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 20 minutes using 1M NaOH | | **F45** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 25 minutes using 1M NaOH | | **F46** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 30 minutes using 1M NaOH | | **F47** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 60 minutes using 1M NaOH | | **F48** | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 90 minutes using 1M NaOH | | **F49** | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 40̊C using 1M HCl | | **F50** | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 40̊C using 1M HCl | | **F51** | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 40̊C using 1M HCl | | **F52** | Acidic degradation product of 10.0 µg/mL FLR after 20 min at 40̊C using 1M HC | | **F53** | Acidic degradation product of 10.0 µg/mL FLR after 25 min at 40̊C using 1M HCl | | **F54** | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 40̊C using 1M HCl | | **F55** | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 60̊ C using 1M HCl | | **F56** | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 60̊ C using 1M HCl | | **F57** | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 60̊ C using 1M HCl | | **F58** | Acidic degradation product of 10.0 µg/mL FLR after 25 min at 60̊ C using 1M HCl | | **F59** | Acidic degradation product of 10.0 µg/mL FLR after 45 min at 60̊ C using 1M HCl | | **F60** | Acidic degradation product of 10.0 µg/mL FLR after 60 min at 60̊ C using 1M HCl | | **F61** | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 60̊ C using 1M HCl | | **F62** | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 80̊ C using 1M HCl | | **F63** | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 80̊ C using 1M HCl | | **F64** | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 80̊ C using 1M HCl | | **F65** | Acidic degradation product of 10.0 µg/mL FLR after 20 min at 80̊ C using 1M HCl | | **F66** | Acidic degradation product of 10.0 µg/mL FLR after 30 min at 80̊ C using 1M HCl | | **F67** | Acidic degradation product of 10.0 µg/mL FLR after 60 min at 80̊ C using 1M HCl | | **F68** | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 80̊ C using 1M HCl | | **F69** | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 100̊C using 1M HCl | | **F70** | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 100̊C using 1M HCl | | **F71** | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 100̊C using 1M HCl | | **F72** | Acidic degradation product of 10.0 µg/mL FLR after 45 min at 100̊C using 1M HCl | | **F73** | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 100̊C using 1M HCl | | **F74** | Oxidative degradation product of 10.0µg/mL after 5 min using 0.07%H<sub>2</sub>O<sub>2</sub> at 40̊C | | **F75** | Oxidative degradation product of 10.0µg/mL after 15 min using 0.07%H2O2 at 40̊C | | **F76** | Oxidative degradation product of 10.0µg/mL after 30 min using 0.07%H2O2 at 40̊C | | **F77** | Oxidative degradation product of 10.0µg/mL after 5 min using 0.07%H2O2 at 100̊C | | **F78** | Oxidative degradation product of 10.0µg/mL after 30 min using 0.07%H2O2 at 100̊C | | **F79** | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=20 nm | | **F80** | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=40 nm | | **F81** | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=60 nm | | **F82** | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=80 nm | | **F83** | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=100 nm | | **F84** | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=120 nm | | **F85** | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=20 nm | | **F86** | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=40 nm | | **F87** | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=60 nm | | **F88** | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=80 nm | | **F89** | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=100 nm | | **F90** | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=120 nm | The selected synchronous fluorescence spectra obtained during forced degradation of FLR. Information on how the mathematical processing was performed to calculate different kinetic data and validation parameters can be found in the associated manuscript referenced above.