cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/nssc5-96948
title: Delftia acidovorans in NB

description: Delftia acidovorans Cs1-4 grown in NB
description: <strong>Tilt Series Date:</strong> 2009-03-30</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Delftia acidovorans</p>
<strong>Strain:</strong> Cs1-4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: 0.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> grown by Bill Hickey at UW-Madison; data collected and processed by Elitza Tocheva</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown in NB.</p>
<strong>Sample Preparation:</strong> 20nm gold, 2s blot, -2 offset</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2009-03-30-23/rawdata/</p>09mar30c_WTonNB_003.mrc,  Tilt Series (Pixel Size 0.962 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2009-03-30-23/rawdata/09mar30c_WTonNB_003.mrc"
        > <i class="download icon"></i> Download </a></p>

        wt3_full.rec,  Reconstruction (Pixel Size 1.924 nm), 570.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2009-03-30-23/3dimage_94710/wt3_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/048sj-qmh07
title: Hyphomonas session11 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-03-29</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-34/rawdata/</p>09mar29a_bx608a_036.mrc,  Tilt Series (Pixel Size 0.632 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-34/rawdata/09mar29a_bx608a_036.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar29a_bx608a_036_full.rec,  Reconstruction (Pixel Size 1.264 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-34/3dimage_7340/09mar29a_bx608a_036_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.b375j8
title: Occurrence Download

description: A dataset containing 2 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Anoectochilus malipoensis"
 ]
}

 The dataset includes 2 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045445-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/2mnbq-s9g18
title: Hyphomonas EB session14 27.5KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-05-22</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-62.0°, 62.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, treated with EB...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-4/rawdata/</p>09may22a_bx200d_007.mrc,  Tilt Series (Pixel Size 0.774 nm), 520.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-4/rawdata/09may22a_bx200d_007.mrc"
        > <i class="download icon"></i> Download </a></p>

        09may22a_bx200d_007_1k.rec,  Reconstruction (Pixel Size 1.548 nm), 59.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-4/3dimage_7592/09may22a_bx200d_007_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9fvjk-g3j41
title: Hyphomonas session16 27.5KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-06-06</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 61.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, 4degree...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-06-35/rawdata/</p>09jun06a_bx823c_011.mrc,  Tilt Series (Pixel Size 0.774 nm), 939.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-06-35/rawdata/09jun06a_bx823c_011.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun06a_bx823c_011_part.rec,  Reconstruction (Pixel Size 1.548 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-06-35/3dimage_7926/09jun06a_bx823c_011_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cs3we-j7w49
title: Hyphomonas session9 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-03-07</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-07-1/rawdata/</p>09mar07a_36c_001.mrc,  Tilt Series (Pixel Size 0.632 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-07-1/rawdata/09mar07a_36c_001.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar07a_36c_001_full.rec,  Reconstruction (Pixel Size 1.264 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-07-1/3dimage_6871/09mar07a_36c_001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y3140-d5m19
title: MT310_xyl(+)_metal(-)

description: Raw data files of MT310_xyl(+)_metal(-)
description: <strong>Tilt Series Date:</strong> 2009-06-09</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> The MT310 strain was constructed by Martin Thanbichler; cells were cultured and treated by Zhuo Li; data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were induced by xylose for 20 hours. No metal salt was added.</p>
<strong>Sample Preparation:</strong> 10 nm gold. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-41/3dimage_5183/</p>09jun09a_Control_061_full.rec,  Reconstruction (Pixel Size 1.9 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-41/3dimage_5183/09jun09a_Control_061_full.rec"
        > <i class="download icon"></i> Download </a></p>

        09jun09a_Control_061.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-09-41/rawdata/09jun09a_Control_061.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5xmbn-cwf76
title: Hyphomonas session8 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-03-06</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 5nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-06-38/rawdata/</p>09mar06a_20d_038.mrc,  Tilt Series (Pixel Size 0.632 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-06-38/rawdata/09mar06a_20d_038.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar06a_20d_038_full.rec,  Reconstruction (Pixel Size 1.264 nm), 377.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-06-38/3dimage_7441/09mar06a_20d_038_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v79fy-ht233
title: Bsubtilis349

description: Bsubtilis JFG349 (MJ007, SpoIIIE78-11-GFP)
description: <strong>Tilt Series Date:</strong> 2011-08-24</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> JFG349, ponA null, SpoIIIE73-11-GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-24-11/3dimage_24953/</p>349a0004_full.rec,  Reconstruction (Pixel Size 1.9 nm), 737.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-24-11/3dimage_24953/349a0004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        349a0004.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2011-08-24-11/rawdata/349a0004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.34740/kaggle/dsv/12871670
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.22002/qg35d-ard14
title: HeLa CHMP4A+VPS4DN

description: HeLa cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-02-08</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with polyfect</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-02-08-4/rawdata/</p>11feb08b_C50-4c_005.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-02-08-4/rawdata/11feb08b_C50-4c_005.st"
        > <i class="download icon"></i> Download </a></p>

        11feb08b_C50-4c_005_full.rec,  Reconstruction (Pixel Size 2.0 nm), 5.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-02-08-4/3dimage_68017/11feb08b_C50-4c_005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/avqy8-sq257
title: CFP-RodZ oe

description: Raw data files of CFP-RodZ oe
description: <strong>Tilt Series Date:</strong> 2009-03-25</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, IMOD</p>
<strong>Collaborators and Roles:</strong> The plasmid was provided by Anisah Alyahya (Jacobs-Wagner lab). Transfection, data collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> A plasmid pJS14pxylcfp-rodZ was electroporated into CB15N. The cells were then grown in PYE plates containing antibiotics. Single clony was picked and then induded by xylose for 24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-03-25-31/rawdata/</p>09mar25a_bx771RGoe_031.mrc,  Tilt Series (Pixel Size 0.632 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-03-25-31/rawdata/09mar25a_bx771RGoe_031.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar25a_bx771RGoe_031_part.rec,  Reconstruction (Pixel Size 1.264 nm), 629.1 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vgr83-b0b80
title: MT310_xyl(+)_AuCl50

description: Raw data files of MT310_xyl(+)_AuCl50
description: <strong>Tilt Series Date:</strong> 2009-06-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> The MT310 strain was constructed by Martin Thanbichler; cell cultured and treated by Zhuo Li; data collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MT310 cells were induced with xylose and treated by 50 uM AuCl for 20 hours. </p>
<strong>Sample Preparation:</strong> 10 nm gold. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-21-49/rawdata/</p>09jun21a_Au50615_057.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-21-49/rawdata/09jun21a_Au50615_057.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun21a_Au50615_057_part.rec,  Reconstruction (Pixel Size 1.9 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-06-21-49/3dimage_4955/09jun21a_Au50615_057_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xkqbw-0vk14
title: Correlatve LMEM stalkbands

description: LMEM experiment of Caulobacter stalkbands
description: <strong>Tilt Series Date:</strong> 2010-12-27</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: -12.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD, PHOTOSHOP for the overlay with the LM image</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grow SW30 overnight in PYE. induce in phosphate limiting M2G - P medium with Xylose for 24 hours. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-12-27-16/rawdata/</p>10dec27a_SBLMEM_005.st,  Tilt Series (Pixel Size 0.0 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-12-27-16/rawdata/10dec27a_SBLMEM_005.st"
        > <i class="download icon"></i> Download </a></p>

        10dec27a_SBLMEM_005.rec,  Reconstruction (Pixel Size 0.0 nm), 146.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2010-12-27-16/3dimage_15087/10dec27a_SBLMEM_005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/45g9c-tyt34
title: Sulfolobus cells synchronized for division

description: search for ESCRT in dividing cells
description: <strong>Tilt Series Date:</strong> 2010-12-03</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Å²,  defocus: -15.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Brock's Medium at 80C, synchronize with Baby Machine</p>
<strong>Sample Preparation:</strong> frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2010-12-03-14/rawdata/</p>10dec03f_Box13grid1_009new.mrc,  Tilt Series (Pixel Size 1.258 nm), 1.1 GB  
        
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        10dec03f_Box13grid1_009new_part.rec,  Reconstruction (Pixel Size 2.38 nm), 1.0 GB  
        
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