cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/m1ehf-x5g95
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-27-5_slicer4252.jpg: Nucleoids ycw2013-09-27-5_slicer4254.jpg: granules ycw2013-09-27-5_slicer4261.jpg: external vesicle ycw2013-09-27-5_slicer4263.jpg: unknown ycw2013-09-27-5_slicer4264.jpg: unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-5/rawdata/</p>20130827_YWC034_10005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-5/rawdata/20130827_YWC034_10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130827_YWC034_10005_part131_8.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-5/3dimage_31313/20130827_YWC034_10005_part131_8.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rjxsy-m4h75
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-72_slicer14285.jpg: Bdellovibrio bacteriovorus + E coli mini cell .  density at interface of two cells ycw2013-09-28-72_slicer14286.jpg: Bdellovibrio bacteriovorus .  Flagellum losing its sheath ycw2013-09-28-72_slicer14287.jpg: Bdellovibrio bacteriovorus .  Flagellum disassembly?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-72/rawdata/</p>20130928_YWC034_10080.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-72/rawdata/20130928_YWC034_10080.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10080_part131_16.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-72/3dimage_31499/20130928_YWC034_10080_part131_16.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16991041
title: Truck Head and Tail Dataset  for Automatic Identification of Truck Head and Tail Using YOLOv10
DOI: 10.22002/se8db-rze23
title: Bdellovibrio/E. coli minicell

description: Sample box: YWC053Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion: mixing time 36 min
description: <strong>Tilt Series Date:</strong> 2013-12-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 36 min. Pellet of 10 nm gold beads were first resuspent thoroughly and 20 ul cells were then gently mixed in by tapping the tube</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-12-06-19_slicer6648.jpg: Attack patch
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-19/rawdata/</p>montage_25x250011.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-19/rawdata/montage_25x250011.mrc"
        > <i class="download icon"></i> Download </a></p>

        montage_25x250011_part131_2.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-06-19/3dimage_35763/montage_25x250011_part131_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j8spg-z6n95
title: H.pylori 26695 delta-cagH / AGS

description: grid box: YWC080
description: <strong>Tilt Series Date:</strong> 2014-02-14</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori & AGS cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-02-14-22_slicer3699.jpg: Helicobacter pylori 26695 delta-cagH co-cultured with AGS cells. .   .  Filament bundles.  Can see some filaments in cross section. ycw2014-02-14-22_slicer9854.jpg: Helicobacter pylori .  chemoreceptor ycw2014-02-14-22_slicer9855.jpg: Helicobacter pylori .  REZ ribosome exclusion zone
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-22/rawdata/</p>20140214_YWC080_10019.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-22/rawdata/20140214_YWC080_10019.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140214_YWC080_10019_part121_43.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-14-22/3dimage_38958/20140214_YWC080_10019_part121_43.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.bmyad3
title: Occurrence Download

description: A dataset containing 738 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lonchura punctulata",
 "Country is Japan",
 "HasCoordinate is true"
 ]
}

 The dataset includes 738 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006441-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.5srd9q
title: Occurrence Download

description: A dataset containing 349 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Lonchura punctulata",
 "Country is Mexico",
 "HasCoordinate is true"
 ]
}

 The dataset includes 349 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006442-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/dqd2z-dvg70
title: Bdellovibrio2492/E.coli minicell

description: Bdellovibrio HI Strain 2492 in mixture with E. coli WM3433 minicellsGrid box YWC107
description: <strong>Tilt Series Date:</strong> 2014-07-18</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio HI 2492 grown from frozen stocks in liquid PY media at 37C for 7 days, subculturing half of culture into equal volume of fresh media every two days. E. coli minicells WM3433 grown O/N to high OD and spun down 7 mins at 6krpm.  Supernatant spun 10 mins at 14krpm to pellet minicells.  Minicells resuspended in 20ul media and combined in 1:1 v/v ratio with Bdellovibrio.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. Manual blotting, 3 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2014-07-18-18_slicer10473.jpg: Bdellovibrio bacteriovorus HI 2492 in contact with WM3433 E. coli minicell.  Arrow marks denser region between two cells.  Possible barrel?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-18/rawdata/</p>20140718_Janelia_YWC107_10016.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-18/rawdata/20140718_Janelia_YWC107_10016.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140718_Janelia_YWC107_10016_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-18/3dimage_45118/20140718_Janelia_YWC107_10016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ks2zg-qfy05
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-03-31-35_slicer9889.jpg: Helicobacter pylori  .  periplasmic filament ycw2014-03-31-35_slicer9888.jpg: Helicobacter pylori  .  extracellular vesicle ycw2014-03-31-35_slicer17882.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-35/rawdata/</p>20140331_YWC097_10035.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-35/rawdata/20140331_YWC097_10035.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10035_part87_4.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-35/3dimage_40688/20140331_YWC097_10035_part87_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/66x2m-r7x56
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-29_slicer10340.jpg: Bdellovibrio bacteriovorus .  chemoreceptor .  flagellar motor .  REZ ycw2013-09-28-29_slicer14238.jpg: Bdellovibrio bacteriovorus .  interesting ripple in membrane ycw2013-09-28-29_slicer14239.jpg: Bdellovibrio bacteriovorus .  flagellum (its end is also visible in full tomogram) .  Flagellar motor with arrows pointing to c-ring?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-29/rawdata/</p>20130928_YWC034_10029.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-29/rawdata/20130928_YWC034_10029.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10029_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-29/3dimage_31405/20130928_YWC034_10029_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/myjag-hbb72
title: Hylemonella + Bdellovibrio

description: Raw data files of Hylemonella + Bdellovibrio
description: <strong>Tilt Series Date:</strong> 2013-01-03</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Hylemonella grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, incubated with attack-phase Bdellovibrio for 3 days, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2013-01-03-12_slicer6365.jpg: Unknown ycw2013-01-03-12_slicer6368.jpg: Unknown ycw2013-01-03-12_slicer6369.jpg: Flagellar motor ycw2013-01-03-12_slicer6374.jpg: Unknown Ribosome cluster .  [2014-07-18 10:56:52] ringsnucleoidflagellar bundle? ycw2013-01-03-12_slicer6377.jpg: REZ ycw2013-01-03-12_slicer6378.jpg: filament ycw2013-01-03-12_slicer6379.jpg: unknown filament ycw2013-01-03-12_slicer6380.jpg: Vesicles ycw2013-01-03-12_slicer6383.jpg: nested vesicle .  [2014-08-06 14:06:17] Hylemonella gracilis ycw2013-01-03-12_slicer6387.jpg: Unknown tube ycw2013-01-03-12_slicer6392.jpg: External Vesicle ycw2013-01-03-12_slicer8063.jpg: inclusion body .  Bdellovibrio and Hylemonella
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-12/rawdata/</p>Bdel_Hyle_0020002.mrc,  Tilt Series (Pixel Size 0.4 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-12/rawdata/Bdel_Hyle_0020002.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdel_Hyle_0020002_full.rec,  Reconstruction (Pixel Size 1.6 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-12/3dimage_24275/Bdel_Hyle_0020002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/35edh-kaz25
title: H.pylori 26695 WT / AGS

description: Grid box: YWC097
description: <strong>Tilt Series Date:</strong> 2014-03-31</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
  ycw2014-03-31-19_slicer3792.jpg: Helicobacter pylori 26695 co-cultured with AGS cells .   .  Short tube on Hp cell .   .  LR ycw2014-03-31-19_slicer9743.jpg: Helicobacter pylori  .  Chemoreceptors
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-19/rawdata/</p>20140331_YWC097_10019.mrc,  Tilt Series (Pixel Size 4.8 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-19/rawdata/20140331_YWC097_10019.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140331_YWC097_10019_full.rec,  Reconstruction (Pixel Size 19.2 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-31-19/3dimage_40656/20140331_YWC097_10019_full.rec"
        > <i class="download icon"></i> Download </a></p>