DOI: 10.5061/dryad.gf1vhhmxn
title: Data from: Hybridization has localized effect on genetic variation in closely related pine species

description: Background: Hybridization is a known phenomenon in nature but its genetic
 impact on populations of parental species remains less understood. We
 investigated the evolutionary consequences of the interspecific gene flow
 in several contact zones of closely related pine species. Using a set of
 genetic markers from both nuclear and organellar genomes, we analyzed a
 large panel of reference allopatric populations of parental taxa (96
 stands, 2601 individuals).   Results: We observed reduced genetic
 diversity in maternally transmitted mitochondrial genomes of pure pine
 species and hybrids from contact zones compared to reference allopatric
 populations. The distribution of mtDNA haplotypes followed geographic
 rather than species boundaries. Additionally, no new haplotypes emerged in
 the contact zones, instead, these zones contained the most common local
 variants. However, species diverged significantly at nuclear genomes, and
 populations in contact zones exhibited similar or higher genetic diversity
 compared to the reference stands. There were no signs of admixture in any
 allopatric population, while clear admixture was evident in the contact
 zones, indicating that hybridization has a localized effect on the genetic
 variation of the analyzed pine species.  Conclusions: Our results
 suggest that hybrid zones act as sinks rather than melting pots of genetic
 diversity. Hybridization influences sympatric populations but is confined
 to contact zones. The spectrum of parental species ancestry in hybrids
 reflects the old evolutionary history of the sympatric populations. These
 findings also imply that introgression may play a crucial role in the
 adaptation of hybrids to specific environments. 
description: # Data from: Hybridization has localized effect on genetic variation in
 closely related pine species
 [https://doi.org/10.5061/dryad.gf1vhhmxn](https://doi.org/10.5061/dryad.gf1vhhmxn) ## Description of the data and file structure Genetic data is divided in two files - one for nuclear and one for mitochondrial data. Nuclear data is a set of 14 polymorphic microsatellites designed for pines. Mitochondrial data is a set of 13 markers (Single Nucleotide Polymorphisms (SNPs) and an indel marker coded as a SNP) generated using a SNaPshot approach. The first two rows of both files contain population and marker information generated by GenAlEx. ### Files and variables #### File: mtDNA.xlsx **Description:** The mtDNA genotypes of 2601 pine individuals scored at 13 polymorphic mtDNA regions. The column sample contains individual ID, and pop population ID. #### File: nSSR.xlsx **Description:** The table contains genotype data scored from 2073 pine individuals across 73 populations. Rounded allele values are provided for 14 SSR loci. The first two columns are for individual ID and population ID, respectively, and the remaining columns are for the 14 SSR loci (2 columns per locus). The table includes a header with column names. ## Code/software Both files can be open using Microsoft Excel and are prepared in GenAlEx 6.5 format.