DOI: 10.6084/m9.figshare.22567558.v1
title: SRK and SCR reference sequences for running the NGSgenotyp pipeline

description: <em><strong>Reference SRK and SCR sequences from Arabidopsis and Capsella</strong></em>
Obtaining S-locus genotypes using the NGSgenotyp pipeline (Genete et al. 2020) requires a series of reference <em>SRK </em>and/or <em>SCR </em>sequences. Here we post fasta files with reference sequences for SRK and SCR, separately. These were obtained by assembling data from the literature. For <em>A. halleri</em>, the reference <em>SRK</em> sequences were taken from Table S1 of Genete et al. (2020). For <em>A. lyrata</em> they were taken from Table S14 of Mable et al. (2018) and from Takou et al. (2021). For <em>C. grandiflora </em>we used a set of partial S-domain sequences obtained by Paetsch et al. (2006), Bechsgaard & Paetsch 2020, Guo et al. 2009, Bachmann et al. 2019, Nasrallah et al. 2007 and Neuffer et al. 2023. Reference sequences of <em>SCR</em> from <em>A. halleri </em>and <em>A. lyrata</em> were taken from Guo et al. (2011), Goubet et al. (2012), and Durand et al. (2014). 
Each file of reference sequences is accompanied by an Excel file with Genbank accession numbers of the data. When the sequences were obtained from de novo assembly made by previous runs of the NGSgenotyp pipeline, we give the SRA accession number of the raw reads individual data used to prduce the corresponding SRK or SCR sequence.
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<em><strong>New SRK and SCR sequences from Capsella species obtained with the NGSgenotyp pipeline</strong></em>
We also post the new or extended sequences of SRK and SCR obtained in Capsella grandiflora or C. bursa-pastoris by applying the NGSgenotyp pipelline on RNAseq or genomic raw Illumina datasets.
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