DOI: 10.5061/dryad.v41ns1s3b
title: Images of flowers and tubers produced by potato recombinant inbred lines

description: Recombinant inbred line (RIL) populations are powerful mapping tools in
 many crops but have not yet been created using cultivated potato
 germplasm. We crossed the doubled monoploid cultivated clone DM 1-3 with
 the self-compatible diploid inbred wild clone M6 to create a diploid F1
 hybrid. One F1 plant was self-pollinated to generate a phenotypically
 diverse F2 population, which was selfed to create 87 RILs. This is the
 first report of a RIL population developed from a cultivated x wild hybrid
 in potato. Poor fertility was a significant challenge in creating RILs.
 Nevertheless, we generated inbred lines that ranged from high to low
 fertility, vigor, and tuber production. F6 RILs ranged from 98% to 68%
 homozygosity, based on 2884 SNP markers. Considering the phenotypic
 variability between the two parents and among the RILs, we expect the RIL
 population to be valuable for mapping traits important to the potato
 industry.
description: We crossed the diploid potato inbred <em>Solanum
 </em>chacoense clone M6 as a male to the <em>S.
 tuberosum</em> doubled monoploid cultivated potato DM 1-3 516 R44 to
 create an F1 population. We then self-pollinated one F1 plant to generate
 an F2 population. We then self-pollinated F2 plants to begin RIL
 development. In the fall of 2013, we sowed approximately 50 seedlings of
 each of 85 F3 families and four weeks later, transplanted 12 seedlings of
 each to 10 cm square pots filled with soilless potting mix. In most cases,
 our goal of at least 12 F3 seedlings was achieved, but low seed numbers,
 poor germination, and low vigor sometimes limited seedling numbers or
 caused the extinction of a lineage. Plants were maintained in a greenhouse
 with a 16-hour photoperiod and day/night temperatures of 24/18C. On every
 plant that flowered, self-pollinations were attempted by holding a pollen
 capsule over each open flower and vibrating it with an electric toothbrush
 in which the brush had been replaced by a plastic-coated wire.
 Pollinations were carried out at least twice per week and in most cases,
 all flowers on each plant were self-pollinated until berries were observed
 on at least two inflorescences. A few plants produced hundreds of flowers;
 at least 100 self-pollinations were attempted on those plants. Performing
 pollinations across many days was helpful, as pollen shed in some plants
 varied over time. Berries were collected at least three weeks after
 pollinating, allowed to ripen at room temperature for three more weeks,
 and then seeds were extracted in water, air-dried, and stored in a -20C
 freezer. Additional F3 families were sown and, if available, additional
 seeds were sown for families that did not generate F4 seeds. This process
 was repeated for additional generations. After the F3
 generation, we grew 18 plants per family instead of 12. For families that
 did not produce any selfed berries, additional sowings were made as long
 as residual seeds were available. Two F2 clones (125 and 159) produced
 highly fertile F3 families; several F4 families were produced, so three
 inbred families were produced from each of these two F2 lineages.
 Otherwise, each F2 plant produced only one recombinant inbred line. Across
 the course of RIL development, 212 F3 families were generated from 253 F2
 plants; 170, 92, and 72 lineages advanced to the F4, F5, and F6
 generations, respectively. The RIL population described here is comprised
 of 87 inbred lines (72 F6, 7 F5, and 8 F4).  In
 February 2020, one tuber of each of the 87 RIL clones was planted in a
 soilless potting mix in 15 cm diameter pots. They were grown in a
 greenhouse with an 18-hour photoperiod and with 50 cm spacing. Plants were
 staked as needed throughout the growing period. As each plant flowered, a
 sample of mature flowers was collected and photographed. On July 2, all
 pots were harvested and photographed, and then tubers were collected,
 washed, and photographed.
description: # Images of flowers and tubers produced by potato recombinant inbred lines
 [https://doi.org/10.5061/dryad.v41ns1s3b](https://doi.org/10.5061/dryad.v41ns1s3b) ## Description of the data and file structure A diploid potato interspecific hybrid (*Solanum chacoense* M6 x *S. tuberosum* DM 1-3 516 R44) was created and then self-pollinated to create a population of recombinant inbred lines. In February 2020, one tuber of each of the 87 recombinant inbred clones was planted in soilless potting mix in 15 cm diameter pots.  They were grown in a greenhouse with an 18-hour photoperiod and with 50 cm spacing.  As each plant flowered, a sample of an individual mature flower and an inflorescence was collected and photographed. These images are presented in the first and second folders.  On July 2, all pots were harvested and the unpotted plants were photographed; then tubers were harvested, washed, and photographed. The washed tubers and unpotted plants are presented in the third and fourth folders. The image name is the U.S. Potato Genebank accession number. Ruler shows millimeters.