cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9598997.v1
title: Biological activity and quantification of bioative compounds in yerba mate extract and its application in fish hamburger

description: Abstract The yerba mate has a high content of biologically active compounds with industrial application. Caffeine, rutin and chlorogenic acid present in this product have antioxidant activity and the latter one is also an antimicrobial agent. This \paper aimed to assess the biological activity of yerba mate extract and quantify its bioactive compounds, as well as apply the extract in fish hamburger to determine its preservative activity. The antioxidant power was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power). Total phenolic compounds and flavonoids were quantified by spectrophotometry, and caffeine, rutin and chlorogenic acid by ultra-performance liquid chromatography. The antimicrobial activity was assessed by Minimum Inhibitory Concentration (MIC). Associations of extract with the synthetic preservative butyl hydroxyanisole (BHA) were tested. Fish hamburgers incorporated with yerba mate were prepared; centesimal composition, lipid and microbiological oxidation were carried out during 28 days. Results: DPPH IC50 of 7.91 µg mL-1 in the extract, FRAP of 4922.67 µmol for Fe (II) g-1, 104.54 EAG g-1 of total phenolics, 23.11 EQ g-1 of flavonoids, and 49.6, 34.5 and 23.1 mg g-1 of chlorogenic acid, caffeine and rutin, respectively. The extract presented a CIM of 10, 5 and 10 mg mL-1 against Escherichia coli, Staphylococcus aureus and Salmonella enterica, respectively. The combination of extract and BHA reduced the amount of synthetic preservative for the same antioxidant activity and presented an additive effect to S. aureus. The fish hamburgers with 1% of extract or with BHA showed statistical difference in mesophiles counting from the control group. Thus, the yerba mate extract presented biological activity and the growth of mesophiles was inhibited in fish hamburguers incorporated with BHA or 1% of extract, indicating that the quantified phenolic compounds are likely to be preservative agents.
DOI: 10.6084/m9.figshare.9598994.v1
title: Reduced visual acuity screening in a Primary Care Unit

description: Abstract Objective: to determine the frequency of reduced visual acuity in children and adolescents attending the child care program of a primary health care unit. Methods: A cross-sectional study was carried out with 290 children and adolescents aged 5 to 18 years attending a primary health unit in the city of Ribeirão Preto (SP) during the first half of 2018. A structured questionnaire Snellen’s visual acuity table. Results: 290 subjects were evaluated, 53.2% female. Of these, 66 (22.7%) were referred to ophthalmologists, 34 (51.5%) were male and 32 (48.5%) were female. We confirmed 31 cases of refractive errors: astigmatism (35.5%), associated astigmatism (25.8%), hypermetropia (29%) and myopia (9.6%). 24 (77.4%) of patients with reduced visual acuity received prescription for corrective lenses. Conclusions: the prevalence of low visual acuity in the studied sample was 10.7%, with a predominance of astigmatism, and with no statistically significant difference between the sexes. It is important to emphasize the importance of performing comprehensive evaluations in the Primary Health Care programs, especially ophthalmologic screening as one of the most important tools for blindness prevention.
DOI: 10.6084/m9.figshare.9598985
title: Sexual dimorphism in the electric knifefish, Gymnorhamphichthys rondoni (Rhamphichthyidae: Gymnotiformes)

description: ABSTRACT Sexual dimorfism refers to morphological differences between males and females of a species. It may be a result of different selection pressures acting on either or both sexes and may occur in any sexually-reproducing dioecious species, including fishes. We analyzed 63 females and 63 adult males of Gymnorhamphichthys rondoni (Gymnotiformes) collected by us or deposited in museum collections. Sex was identified through abdominal dissection. We measured length from snout to posterior end of anal-fin, anal-fin length, distance from anus to anal-fin origin, distance from genital papilla to anal-fin origin, body width at beginning of anal-fin, and head length. Morphometric data submitted to a Principal Component Analysis (PCA) grouped males and females according to variables related to body size (along the first component) and to head length and body height along the second and third components. Females were larger than males, whereas males had proportionally larger heads and higher bodies than females. The urogenital papilla of males and females showed differences in shape, size and relative position on the body. The female papilla was elongated horizontally, larger than that of males, and was located on a vertical line below the eye, while the papilla of the males was vertically elongated and located on a vertical line below the operculum. To our knowledge, this is the first recorded case of sexual dimorphism in a species of Rhamphichthyidae, a condition that is now known in all the currently recognized families of Gymnotiformes.
DOI: 10.6084/m9.figshare.9598985.v1
title: Sexual dimorphism in the electric knifefish, Gymnorhamphichthys rondoni (Rhamphichthyidae: Gymnotiformes)

description: ABSTRACT Sexual dimorfism refers to morphological differences between males and females of a species. It may be a result of different selection pressures acting on either or both sexes and may occur in any sexually-reproducing dioecious species, including fishes. We analyzed 63 females and 63 adult males of Gymnorhamphichthys rondoni (Gymnotiformes) collected by us or deposited in museum collections. Sex was identified through abdominal dissection. We measured length from snout to posterior end of anal-fin, anal-fin length, distance from anus to anal-fin origin, distance from genital papilla to anal-fin origin, body width at beginning of anal-fin, and head length. Morphometric data submitted to a Principal Component Analysis (PCA) grouped males and females according to variables related to body size (along the first component) and to head length and body height along the second and third components. Females were larger than males, whereas males had proportionally larger heads and higher bodies than females. The urogenital papilla of males and females showed differences in shape, size and relative position on the body. The female papilla was elongated horizontally, larger than that of males, and was located on a vertical line below the eye, while the papilla of the males was vertically elongated and located on a vertical line below the operculum. To our knowledge, this is the first recorded case of sexual dimorphism in a species of Rhamphichthyidae, a condition that is now known in all the currently recognized families of Gymnotiformes.
DOI: 10.6084/m9.figshare.9598967
title: Post-harvest characterization of ‘Prata’ banana cultivar grown under different nitrogen and potassium fertilization

description: Abstract There are several banana cultivars resistant to Black Sigatoka disease and to Panama disease, but due to the lack of information regarding post-harvest characteristics, many are rejected in the market. Nitrogen and potassium fertilization may help to improve the sensory characteristics of ripe banana, but there is lack of studies on its effects on banana cultivation, especially those resistant to diseases. The aim of this study was to evaluate the post-harvest characteristics of ‘Prata-Anã’, ‘BRS Platina’ and ‘PA94-01’ banana cultivars at four N and K2O fertilization levels (NK0: no fertilization, NK1: 175 and 285 kg ha-1 year-1, NK2: 350 and 570 kg ha-1 year-1, NK3: 525 and 855 kg ha-1 year-1, respectively) in three production cycles. It could be concluded that N and K doses adopted in this work did not influence the post-harvest physicochemical characteristics of ‘Prata-Anã’, ‘BRS Platina’ and ‘PA94-01’ banana cultivars. Regardless of N and K doses, ‘Prata-Anã’, ‘BRS Platina’ and PA94-01’ banana cultivars presented differences regarding post-harvest physicochemical characteristics. Production cycles did not influence the physical characteristics but differed in post-harvest chemical characteristics in ‘Prata-Anã’, ‘BRS Platina’ and ‘PA94-01’ banana cultivars.
DOI: 10.6084/m9.figshare.9598961
title: Coarctation of The Aorta: A Case-Series from a Tertiary Care Hospital

description: Abstract Background: Coarctation of the aorta is a congenital segmental narrowing of the aortic arch with severe hemodynamic repercussions and increased cardiovascular mortality. Early surgical correction and life-time echocardiographic follow-up must be performed to improve prognosis. However, this goal has been challenged by high rates of underdiagnosis, which delay surgical correction, and by recoarctation in up to one third of operated patients. Objectives: The objectives of this study were: (i) to register the frequency of common clinical signs at diagnosis of coarctation of the aorta; (ii) to describe the course of echocardiographic parameters before and during the follow-up of coartectomized subjects; (iii) to analyze the clinical prognosis of patients according to baseline characteristics, occurrence of recoarctation and associated malformations. Methods: Case-series of 72 patients coarctectomized between June 1996 and November 2016 in a tertiary care hospital. Clinical, echocardiographic and surgical variables were considered. All patients were submitted to coarctectomy by posterolateral thoracotomy and end-to-end anastomosis. Data were classified as parametric or non-parametric by Kolmogorov-Smirnov test. Parametric data were expressed as mean and standard deviation, and non-parametric data as median and interquartile range. Continuous variables were analyzed using paired t-tests, and categorical variables were compared by chi-square test. For all analysis, a p-value of less than 0.05 was considered statistically significant. Statistical analysis was performed using SPSS, version 20.0 (IBM, Chicago, IL, USA). Results: The mean follow-up time was 5.8 years (range: 0-20 years). At diagnosis, most patients had heart murmur (88%), non-palpable pulse in the lower limbs (50%), left ventricular hypertrophy (78%), and bicuspid aortic valve (33%), with a mean aortic peak gradient of 55 mmHg. After surgical correction, those without recoarctation were less symptomatic (60 vs 4.5%; p &lt; 0.001), had lower aortic peak gradient (54 ± 3.8 vs 13 ± 0.8; p = 0.01) and left ventricle mass (95 ± 9.2 vs. 63 ± 11; p = 0.01), and the most common complications were late hypertension (39.2%), and recoarctation (27.6%). Recoarcted patients did not show improvement of neither clinical nor echocardiographic variables. Age at repair and bicuspid aortic valve groups had comparable results with controls. Surgical procedure was safe; mean time of hospitalization was 10 days and mean surgery time 2.3 hours. Conclusions: Coarctectomy improves cardiac symptoms and left ventricular hypertrophy, with a slight effect on the incidence of hypertension. Recoarctation occurs in one-third of patients and draws attention for the need of lifelong surveillance by echocardiography.
DOI: 10.6084/m9.figshare.9598967.v1
title: Post-harvest characterization of ‘Prata’ banana cultivar grown under different nitrogen and potassium fertilization

description: Abstract There are several banana cultivars resistant to Black Sigatoka disease and to Panama disease, but due to the lack of information regarding post-harvest characteristics, many are rejected in the market. Nitrogen and potassium fertilization may help to improve the sensory characteristics of ripe banana, but there is lack of studies on its effects on banana cultivation, especially those resistant to diseases. The aim of this study was to evaluate the post-harvest characteristics of ‘Prata-Anã’, ‘BRS Platina’ and ‘PA94-01’ banana cultivars at four N and K2O fertilization levels (NK0: no fertilization, NK1: 175 and 285 kg ha-1 year-1, NK2: 350 and 570 kg ha-1 year-1, NK3: 525 and 855 kg ha-1 year-1, respectively) in three production cycles. It could be concluded that N and K doses adopted in this work did not influence the post-harvest physicochemical characteristics of ‘Prata-Anã’, ‘BRS Platina’ and ‘PA94-01’ banana cultivars. Regardless of N and K doses, ‘Prata-Anã’, ‘BRS Platina’ and PA94-01’ banana cultivars presented differences regarding post-harvest physicochemical characteristics. Production cycles did not influence the physical characteristics but differed in post-harvest chemical characteristics in ‘Prata-Anã’, ‘BRS Platina’ and ‘PA94-01’ banana cultivars.
DOI: 10.6084/m9.figshare.9598955
title: Differences in negative predictive value of prostate MRI based in men with suspected or known cancer

description: Abstract Objective: To compare the negative predictive value (NPV) of multiparametric MRI for Gleason score (GS) ≥ 3+4 cancer and evaluate predictors of these tumors in men with suspected disease and under active surveillance (AS). Materials and Methods: This retrospective study included 38 men with suspected prostate cancer and 38 under AS with scans assigned PI-RADS v2 scores 1 or 2 between May 2016 and September 2017. Biopsy results were no cancer, GS = 3+3, or GS ≥ 3+4. Pre-MRI PSA, gland volume, and PSA density were recorded. Chi-square, equality of proportions, and logistic regressions were used to analyze the data. Results: Intermediate to high-grade cancer was found in 12.8% (95% CI = 2.3-23.3) and 35.9% (95% CI = 20.8-50.9) of men with suspected cancer, and under AS (p = 0.02), respectively. The NPV for GS ≥ 3+4 were 87.2% (suspected cancer; 76.7-97.7) and 64.1% (AS; 49.0-79.2). In neither group PSA significantly predicted cancer grade (p = 0.75 and 0.63). Although it did not reach conventional statistical significance, PSA density was a good predictor of cancer grade in men with suspected disease (p = 0.06), but not under AS (p = 0.62). Conclusion: The NPV of multiparametric MRI for GS ≥ 3+4 is higher in men with suspected prostate cancer than in men under AS. PSA density ≤ 0.15 improved the prediction of intermediate to high-grade disease in patients without known cancer.
DOI: 10.6084/m9.figshare.9598958
title: Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

description: Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60 showed smooth fibers with an average diameter of 771±273, 606±242, and 697±232 nm, respectively. When the degradation ratio was evaluated, the three scaffold groups had a similar molecular weight (Mw) on the final day of analysis. PTMCLLA 30/70 and 40/60 scaffolds exhibited greater flexibility than the PTMCLLA 20/80. However, the PTMCLLA 40/60 scaffolds showed a large wrinkling and their biological properties were not evaluated. The PTMCLLA 30/70 scaffolds supported the adhesion and growth of mesenchymal stem cells (MSCs), endothelial progenitor cells, and smooth muscle cells (SMCs). In addition, they provided a spreading of MSCs and SMCs. Given the results, the electrospun scaffolds produced with PTMCLLA 30/70 copolymer can be considered promising candidates for future applications in vascular tissue engineering.
DOI: 10.6084/m9.figshare.9598964
title: “A body shape index” and its association with arterial hypertension and diabetes mellitus among Brazilian older adults: National Health Survey (2013)

description: The aim was to evaluate the separate and joint association of abdominal adiposity indicators (a body shape index - ABSI, waist circumference - WC, waist-to-height ratio - WHtR) and body mass index (BMI) with arterial hypertension and diabetes mellitus, in Brazilian older adults. Data from the 2013 Brazilian National Health Survey (PNS 2013) were used for the population aged 60 years or older (10,537 older adults). Arterial hypertension and diabetes mellitus outcomes were self-reported and the following anthropometric indices were evaluated by direct measurement: a ABSI, BMI, WC and WHtR. Associations were assessed by logistic regression, with adjustments for confounding factors. The results of this study evidenced a higher strength of association between the report of arterial hypertension and diabetes mellitus with BMI, WC and WHtR in the Brazilian population of older adults in separate analyses, when compared to ABSI. When adjusted for BMI, ABSI showed a greater strength of association with the outcomes, but it was not superior to the performance of WC and WHtR. Considering the lower strength of association, in separate and joint analyses, between the new index (ABSI) and the chronic conditions assessed, BMI, WC and WHtR probably remain as useful indices in public health, at least in relation to arterial hypertension and diabetes mellitus in Brazilian older adults.
DOI: 10.6084/m9.figshare.9598958.v1
title: Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

description: Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60 showed smooth fibers with an average diameter of 771±273, 606±242, and 697±232 nm, respectively. When the degradation ratio was evaluated, the three scaffold groups had a similar molecular weight (Mw) on the final day of analysis. PTMCLLA 30/70 and 40/60 scaffolds exhibited greater flexibility than the PTMCLLA 20/80. However, the PTMCLLA 40/60 scaffolds showed a large wrinkling and their biological properties were not evaluated. The PTMCLLA 30/70 scaffolds supported the adhesion and growth of mesenchymal stem cells (MSCs), endothelial progenitor cells, and smooth muscle cells (SMCs). In addition, they provided a spreading of MSCs and SMCs. Given the results, the electrospun scaffolds produced with PTMCLLA 30/70 copolymer can be considered promising candidates for future applications in vascular tissue engineering.
DOI: 10.6084/m9.figshare.9598961.v1
title: Coarctation of The Aorta: A Case-Series from a Tertiary Care Hospital

description: Abstract Background: Coarctation of the aorta is a congenital segmental narrowing of the aortic arch with severe hemodynamic repercussions and increased cardiovascular mortality. Early surgical correction and life-time echocardiographic follow-up must be performed to improve prognosis. However, this goal has been challenged by high rates of underdiagnosis, which delay surgical correction, and by recoarctation in up to one third of operated patients. Objectives: The objectives of this study were: (i) to register the frequency of common clinical signs at diagnosis of coarctation of the aorta; (ii) to describe the course of echocardiographic parameters before and during the follow-up of coartectomized subjects; (iii) to analyze the clinical prognosis of patients according to baseline characteristics, occurrence of recoarctation and associated malformations. Methods: Case-series of 72 patients coarctectomized between June 1996 and November 2016 in a tertiary care hospital. Clinical, echocardiographic and surgical variables were considered. All patients were submitted to coarctectomy by posterolateral thoracotomy and end-to-end anastomosis. Data were classified as parametric or non-parametric by Kolmogorov-Smirnov test. Parametric data were expressed as mean and standard deviation, and non-parametric data as median and interquartile range. Continuous variables were analyzed using paired t-tests, and categorical variables were compared by chi-square test. For all analysis, a p-value of less than 0.05 was considered statistically significant. Statistical analysis was performed using SPSS, version 20.0 (IBM, Chicago, IL, USA). Results: The mean follow-up time was 5.8 years (range: 0-20 years). At diagnosis, most patients had heart murmur (88%), non-palpable pulse in the lower limbs (50%), left ventricular hypertrophy (78%), and bicuspid aortic valve (33%), with a mean aortic peak gradient of 55 mmHg. After surgical correction, those without recoarctation were less symptomatic (60 vs 4.5%; p &lt; 0.001), had lower aortic peak gradient (54 ± 3.8 vs 13 ± 0.8; p = 0.01) and left ventricle mass (95 ± 9.2 vs. 63 ± 11; p = 0.01), and the most common complications were late hypertension (39.2%), and recoarctation (27.6%). Recoarcted patients did not show improvement of neither clinical nor echocardiographic variables. Age at repair and bicuspid aortic valve groups had comparable results with controls. Surgical procedure was safe; mean time of hospitalization was 10 days and mean surgery time 2.3 hours. Conclusions: Coarctectomy improves cardiac symptoms and left ventricular hypertrophy, with a slight effect on the incidence of hypertension. Recoarctation occurs in one-third of patients and draws attention for the need of lifelong surveillance by echocardiography.
DOI: 10.6084/m9.figshare.9598964.v1
title: “A body shape index” and its association with arterial hypertension and diabetes mellitus among Brazilian older adults: National Health Survey (2013)

description: The aim was to evaluate the separate and joint association of abdominal adiposity indicators (a body shape index - ABSI, waist circumference - WC, waist-to-height ratio - WHtR) and body mass index (BMI) with arterial hypertension and diabetes mellitus, in Brazilian older adults. Data from the 2013 Brazilian National Health Survey (PNS 2013) were used for the population aged 60 years or older (10,537 older adults). Arterial hypertension and diabetes mellitus outcomes were self-reported and the following anthropometric indices were evaluated by direct measurement: a ABSI, BMI, WC and WHtR. Associations were assessed by logistic regression, with adjustments for confounding factors. The results of this study evidenced a higher strength of association between the report of arterial hypertension and diabetes mellitus with BMI, WC and WHtR in the Brazilian population of older adults in separate analyses, when compared to ABSI. When adjusted for BMI, ABSI showed a greater strength of association with the outcomes, but it was not superior to the performance of WC and WHtR. Considering the lower strength of association, in separate and joint analyses, between the new index (ABSI) and the chronic conditions assessed, BMI, WC and WHtR probably remain as useful indices in public health, at least in relation to arterial hypertension and diabetes mellitus in Brazilian older adults.