cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7452428.v1
title: Colimetric index and virulence genes iss and iutA in Escherichia coli isolates in cellulitis of poultry carcasses under sanitary inspection

description: SUMMARY Current study determines the population of total coliforms and Escherichia coli and identifies iss and iutA virulence genes in Escherichia coli strains isolated from cellulitis in poultry carcasses retrieved from a slaughterhouse. One hundred cellulitis lesions were collected between August 2013 and January 2014. The population of total coliforms and Escherichia coli was verified by Petrifilm™ rapid counting method (AOAC 998.8). Escherichia coli samples were analyzed for iss and iutA genes by Polymerase Chain Reaction (PCR) technique. Total coliforms were present in 96.0% (96/100) of the analyzed samples, with a population between 3.4 and 9.5 log CFU/g. Escherichia coli was present in 82.0% (82/100) of cellulitis samples and the population ranged between &lt;1.0 and 9.0 log CFU/g. The iss gene was found in 89.0% of isolates and the iutA gene in 97.6%. High populations of total coliforms and Escherichiacoli in cellulitis samples indicate that hygienic-sanitary failures may have occurred in the production of broilers. When high prevalence of virulence genes under analysis, characteristic of Avian Pathogenic Escherichia coli (APEC) and possible zoonotic character of the pathotype are taken into account, it is important to highlight the need to adopt Good Manufacturing Practices, Standard Procedures of Operational Hygiene and Hazard Analysis and Critical Control Points in poultry slaughterhouses to ensure the safety of the final product.
DOI: 10.6084/m9.figshare.7452419.v1
title: From Initial Education to Portuguese L1 Classroom: conceptions about teaching and learning grammar

description: ABSTRACT The goal of this paper is to discuss representations about grammar teaching of Portuguese L1 teachers. It draws on the exploratory study designed to identify the invariants and divergences in self-perceptions about L1 grammar teaching of trainees in different training situations: in-service teachers, pre-service teachers and bachelor’s students. The study focus on student’s and teacher’s acknowledgment of the Portuguese L1 Curriculum (Reis et al. 2009), which conveys a clear paradigm change in Portuguese language education, assuming that grammar teaching is based upon language awareness development. The results show an evident gap between students and teachers in what concerns the familiarity with the Portuguese L1 Curriculum and teaching strategies. However, some puzzling convergent points are the importance attributed to grammar and poor linguistics background. The results analysis draws attention to the relationship between teacher cognition and practice, highlighting fragilities in teacher education and potential conceptual changes that may lead to effective changes in classroom practices.
DOI: 10.7284/101818
title: ADCP (Acoustic Doppler Current Profiler) data collected during research cruise MV1006 using a Hawaii UHDAS instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7452413
title: Effect of association of imagery and physical practice on children’s motor learning

description: Abstract Imagery training has been shown to induce motor learning in adults, but similar evidence in children is scarce. In this experiment, we aimed to evaluate the effect of association between imagery and physical practice compared to pure physical practice in the learning of a manual task in 9-10 year-old children. The task consisted of transporting a block and fitting it into a support with speed and steadiness, assessing movement time to complete the “reaching” and “transport” task components. The children were assigned to one of three groups: (a) physical practice (PHYS) (240 trials), (b) combination (COMB) of imagery (180 trials) and physical (60 trials) practice, and (c) control (CON), associating visual rotation (180 trials) and physical practice (60 trials). Performance was evaluated immediately and 24 h after practice. Results indicated that the PHYS group achieved a persistent performance gain in the “transport”, but not in the “reaching” task component, while the COMB group achieved persistent performance gains in both movement components; no significant differences were found for the CON group. Our results suggest that imagery training improves the task mental representation in children, while physical practice provides sensory feedback on the performed movements. As a conclusion, the results suggest that combination of imagery and physical practice can be more effective than pure physical practice for children’s motor learning.
DOI: 10.6084/m9.figshare.7452404
title: HER2-expressing breast tumors are associated with breast cancer stem-cell phenotype CD44+/CD24-

description: ABSTRACT INTRODUCTION: According to the cancer stem-cell theory, tumors originate from a small population of cancer stem cells, which lose the mechanism of self-regulation and begin to differentiate and proliferate indefinitely. The CD44+/CD24- phenotype may be considered a stem-cell marker in breast cancer. OBJECTIVE: To evaluate the correlation between CD44+/CD24- phenotype and different molecular subtypes of breast cancer in invasive ductal carcinoma samples. METHODS: The expression of CD44, CD44v6, and CD24 markers was investigated in 133 cases of invasive mammary carcinoma with immunohistochemistry. CD44+/CD24- phenotype was identified and correlated with the molecular subtypes and classical prognostic factors such as age, histological grade, tumor size, and lymph node status. RESULTS: Eighteen (14%) cases were positive for CD44+/CD24- (CD44+/CD24- or CD44v6+/CD24-) phenotype; among these, 11.1%, 27.8%, 38.9%, and 22.2% were luminal, luminal B-human epidermal growth factor receptor 2 (HER2), HER2, and triple-negative subtype, respectively. CD44+/ CD24- phenotype was more common in HER2 subgroup (p = 0.0197). CONCLUSION: CD44+/CD24- phenotype was correlated with molecular subtypes of breast cancer. The highest expression of CD44+/CD24- phenotype was reported in patients with HER2+ disease, a molecular subtype associated with more aggressive behavior and worse prognosis.
DOI: 10.6084/m9.figshare.7452407
title: A new termite species of the genus Dihoplotermes Araújo (Blattaria, Isoptera, Termitidae) from the Brazilian Amazonian rainforest

description: ABSTRACT Dihoplotermes, a previously monotypic genus, is now composed of two species. Dihoplotermes taurus sp. nov., a new Termitinae termite species, is illustrated and described based on morphological characters from soldiers, workers, and imago. The new species is distinguished from Dihoplotermes inusitatus Araujo by having a spine-like protuberance with blunt apex on the postmentum, the presence of a spine on the gizzard and the absence of dimorphic soldiers. Furthermore, the internal morphological characteristics of a Dihoplotermes worker are described for the first time. Both internal and external morphological characters were used to improve the characterization of the species.
DOI: 10.6084/m9.figshare.7452410
title: Feeding frequency affects feed intake and growth in juvenile pirarucu (Arapaima gigas)

description: ABSTRACT Pirarucu is one of the main fish species for the development of aquaculture in the Amazon. In this study, the optimal feeding frequency for juvenile pirarucu was assessed based on growth and feed efficiency. Juvenile pirarucu weighing ca. 80 g were fed once, twice, three or four times daily until apparent satiation for 63 days. Fish fed three or four times per day presented higher growth, feed intake, and fat body content than those fed once or twice per day. There was no significant difference for feed conversion ratio, and protein and energy retention rates among treatments, suggesting that increased feeding frequency did not affect feed utilization efficiency. Mean feed intake per meal was higher when fish were fed once per day, possibly causing hyperphagic behavior. Results suggest that feeding three times per day was sufficient to secure adequate feeding and growth of juvenile pirarucu.
DOI: 10.6084/m9.figshare.7452413.v1
title: Effect of association of imagery and physical practice on children’s motor learning

description: Abstract Imagery training has been shown to induce motor learning in adults, but similar evidence in children is scarce. In this experiment, we aimed to evaluate the effect of association between imagery and physical practice compared to pure physical practice in the learning of a manual task in 9-10 year-old children. The task consisted of transporting a block and fitting it into a support with speed and steadiness, assessing movement time to complete the “reaching” and “transport” task components. The children were assigned to one of three groups: (a) physical practice (PHYS) (240 trials), (b) combination (COMB) of imagery (180 trials) and physical (60 trials) practice, and (c) control (CON), associating visual rotation (180 trials) and physical practice (60 trials). Performance was evaluated immediately and 24 h after practice. Results indicated that the PHYS group achieved a persistent performance gain in the “transport”, but not in the “reaching” task component, while the COMB group achieved persistent performance gains in both movement components; no significant differences were found for the CON group. Our results suggest that imagery training improves the task mental representation in children, while physical practice provides sensory feedback on the performed movements. As a conclusion, the results suggest that combination of imagery and physical practice can be more effective than pure physical practice for children’s motor learning.
DOI: 10.6084/m9.figshare.7452404.v1
title: HER2-expressing breast tumors are associated with breast cancer stem-cell phenotype CD44+/CD24-

description: ABSTRACT INTRODUCTION: According to the cancer stem-cell theory, tumors originate from a small population of cancer stem cells, which lose the mechanism of self-regulation and begin to differentiate and proliferate indefinitely. The CD44+/CD24- phenotype may be considered a stem-cell marker in breast cancer. OBJECTIVE: To evaluate the correlation between CD44+/CD24- phenotype and different molecular subtypes of breast cancer in invasive ductal carcinoma samples. METHODS: The expression of CD44, CD44v6, and CD24 markers was investigated in 133 cases of invasive mammary carcinoma with immunohistochemistry. CD44+/CD24- phenotype was identified and correlated with the molecular subtypes and classical prognostic factors such as age, histological grade, tumor size, and lymph node status. RESULTS: Eighteen (14%) cases were positive for CD44+/CD24- (CD44+/CD24- or CD44v6+/CD24-) phenotype; among these, 11.1%, 27.8%, 38.9%, and 22.2% were luminal, luminal B-human epidermal growth factor receptor 2 (HER2), HER2, and triple-negative subtype, respectively. CD44+/ CD24- phenotype was more common in HER2 subgroup (p = 0.0197). CONCLUSION: CD44+/CD24- phenotype was correlated with molecular subtypes of breast cancer. The highest expression of CD44+/CD24- phenotype was reported in patients with HER2+ disease, a molecular subtype associated with more aggressive behavior and worse prognosis.
DOI: 10.6084/m9.figshare.7452407.v1
title: A new termite species of the genus Dihoplotermes Araújo (Blattaria, Isoptera, Termitidae) from the Brazilian Amazonian rainforest

description: ABSTRACT Dihoplotermes, a previously monotypic genus, is now composed of two species. Dihoplotermes taurus sp. nov., a new Termitinae termite species, is illustrated and described based on morphological characters from soldiers, workers, and imago. The new species is distinguished from Dihoplotermes inusitatus Araujo by having a spine-like protuberance with blunt apex on the postmentum, the presence of a spine on the gizzard and the absence of dimorphic soldiers. Furthermore, the internal morphological characteristics of a Dihoplotermes worker are described for the first time. Both internal and external morphological characters were used to improve the characterization of the species.
DOI: 10.6084/m9.figshare.7452410.v1
title: Feeding frequency affects feed intake and growth in juvenile pirarucu (Arapaima gigas)

description: ABSTRACT Pirarucu is one of the main fish species for the development of aquaculture in the Amazon. In this study, the optimal feeding frequency for juvenile pirarucu was assessed based on growth and feed efficiency. Juvenile pirarucu weighing ca. 80 g were fed once, twice, three or four times daily until apparent satiation for 63 days. Fish fed three or four times per day presented higher growth, feed intake, and fat body content than those fed once or twice per day. There was no significant difference for feed conversion ratio, and protein and energy retention rates among treatments, suggesting that increased feeding frequency did not affect feed utilization efficiency. Mean feed intake per meal was higher when fish were fed once per day, possibly causing hyperphagic behavior. Results suggest that feeding three times per day was sufficient to secure adequate feeding and growth of juvenile pirarucu.
DOI: 10.7284/101817
title: Expendable Probe data collected during research cruise MV1006 using a Sippican MK21 instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7452395
title: Evaluation of the accuracy of frozen section in different anatomical sites

description: ABSTRACT INTRODUCTION: Frozen section is recommended in several situations to: establish the nature of a lesion; establish the presence of a lesion; confirm the presence of a benign lesion; confirm that sufficient tissue is present for diagnosis; establish the grade of the lesion; determine the organ of origin and determine the adequacy of margins. OBJECTIVES: To evaluate the accuracy of frozen section biopsy in multiple organs and analyze possible factors in discrepancy. METHODS: A retrospective study was carried out during a five-year period at a teaching hospital of Recife, Pernambuco, Brazil. The diagnoses of frozen section were compared with results obtained in the permanent section and classified as concordant or discordant. The discordant cases were reviewed by a pathologist and subdivided into false positives and false negatives. Possible reasons for discrepancy were indicated. RESULTS: A total of 1.226 specimens were analyzed, of which 1.181 (96.33%) were concordant and 45 (3.67%) were discordant. After the review of the discordant cases, 39 remained, six (15.4%) were false positives and 33 (84.6%) were false negatives. The tissue that had most false-positive results was mammary sentinel lymph node (3/1.2%), whereas ovarian showed most false negative outcomes with 17 specimens (51.51% of all false negatives). The possible reasons for discrepancy were sampling error, misunderstanding and complexity of the diagnosis. CONCLUSION: The frozen section accuracy of 96.3% found in our study is similar to specialized literature and does not seem to depend on the tissue analyzed.
DOI: 10.6084/m9.figshare.7452398
title: Developing a Soil Physical Quality Index (SPQi) for lowlands under different deployment times of no-tillage

description: ABSTRACT: Soil physical quality in lowlands from the Pampa biome under no-tillage (NT) plays an important role; therefore, this study aimed to establish a soil physical quality index (SPQi) from a minimum data set to detect the effects of different deployment times of NT in an Albaqualf. The comparison of areas with one (NT1), three (NT3), five (NT5) and seven (NT7) years of notillage was established using as reference a non-cultivated field plot (NC) for at least thirty years, nearby the sites under NT. Soil samples with undisturbed and disturbed structure were collected to determine the physical quality indicators and soil organic matter (SOM) fractions. The factor analysis (FA) was used to identify and select a minimum data set. The SPQi was elaborated by using the deviations of the measured indicators at different deployment times of NT in relation to NC. The SPQi showed sensibility to identify and explain soil physical quality changes with different deployment times of NT. In well-drained lands, higher deployment times of no-tillage promote the physical quality of lowlands.
DOI: 10.6084/m9.figshare.7452398.v1
title: Developing a Soil Physical Quality Index (SPQi) for lowlands under different deployment times of no-tillage

description: ABSTRACT: Soil physical quality in lowlands from the Pampa biome under no-tillage (NT) plays an important role; therefore, this study aimed to establish a soil physical quality index (SPQi) from a minimum data set to detect the effects of different deployment times of NT in an Albaqualf. The comparison of areas with one (NT1), three (NT3), five (NT5) and seven (NT7) years of notillage was established using as reference a non-cultivated field plot (NC) for at least thirty years, nearby the sites under NT. Soil samples with undisturbed and disturbed structure were collected to determine the physical quality indicators and soil organic matter (SOM) fractions. The factor analysis (FA) was used to identify and select a minimum data set. The SPQi was elaborated by using the deviations of the measured indicators at different deployment times of NT in relation to NC. The SPQi showed sensibility to identify and explain soil physical quality changes with different deployment times of NT. In well-drained lands, higher deployment times of no-tillage promote the physical quality of lowlands.
DOI: 10.6084/m9.figshare.7452392
title: Metabolic syndrome in bipolar disorder: prevalence, demographics and clinical correlates in individuals with bipolar I, bipolar II, and healthy controls

description: Abstract Background: The metabolic syndrome is a growing global public health problem and highly prevalent in patiens with bipolar disorder. There are a few studies about relationship between metabolic syndrome and bipolar disorder subtypes. Objective: The aim of this study was to investigate the prevalence of metabolic syndrome (MS) and its individual components in subjects with bipolar I (BD I) and bipolar II (BD II) disorder compared with non-psychiatric controls, and to determine the variables affecting MS. Methods: A total of 210 individuals (mean age 42.5 ± 11.87, 58.1% female) of whom 70 had BD I, 70 BD II, and 70 controls, were included in this study. MS was diagnosed according to the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III), the adapted ATP III (ATP III-A) and the International Diabetes Federation (IDF) criteria. Results: Participants with BD I had a significantly higher prevalence of MS when compared to individuals BD II and non-psychiatric controls according to the NCEP-ATP III, ATP III-A, and IDF criteria (ps &lt; 0.01). In individuals with MS, increased waist circumference was the most common abnormality. Logistic regression analysis revealed that the presence of physical illness, age and number of cigarettes smoked significantly predicted the presence of MS. Discussion: This study showed that MS was more prevalent among BD I individuals compared to BD II and controls, and highlighted the importance of regular screening for MS in individuals with BD.
DOI: 10.6084/m9.figshare.7452395.v1
title: Evaluation of the accuracy of frozen section in different anatomical sites

description: ABSTRACT INTRODUCTION: Frozen section is recommended in several situations to: establish the nature of a lesion; establish the presence of a lesion; confirm the presence of a benign lesion; confirm that sufficient tissue is present for diagnosis; establish the grade of the lesion; determine the organ of origin and determine the adequacy of margins. OBJECTIVES: To evaluate the accuracy of frozen section biopsy in multiple organs and analyze possible factors in discrepancy. METHODS: A retrospective study was carried out during a five-year period at a teaching hospital of Recife, Pernambuco, Brazil. The diagnoses of frozen section were compared with results obtained in the permanent section and classified as concordant or discordant. The discordant cases were reviewed by a pathologist and subdivided into false positives and false negatives. Possible reasons for discrepancy were indicated. RESULTS: A total of 1.226 specimens were analyzed, of which 1.181 (96.33%) were concordant and 45 (3.67%) were discordant. After the review of the discordant cases, 39 remained, six (15.4%) were false positives and 33 (84.6%) were false negatives. The tissue that had most false-positive results was mammary sentinel lymph node (3/1.2%), whereas ovarian showed most false negative outcomes with 17 specimens (51.51% of all false negatives). The possible reasons for discrepancy were sampling error, misunderstanding and complexity of the diagnosis. CONCLUSION: The frozen section accuracy of 96.3% found in our study is similar to specialized literature and does not seem to depend on the tissue analyzed.
DOI: 10.7284/101813
title: GNSS (Global Navigation Satellite System) data collected during research cruise MV1006 using a Furuno GP-90D instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7452383
title: Antiplasmodial and antileishmanial activities of compounds from Piper tuberculatum Jacq fruits

description: Abstract INTRODUCTION This study assessed the activity of compounds from Piper tuberculatum against Plasmodium falciparum and Leishmania guyanensis. METHODS The effects of compounds from P. tuberculatum fruits on P. falciparum and L. guyanensis promastigote growth in vitro were determined. Hemolytic action and cytotoxicity in HepG2 and J774 cells were measured. RESULTS Three compounds showed strong antiplasmodial activity and one compound showed strong antileishmanial activity. Two compounds were non-toxic to HepG2 cells and all were toxic to J774 cells. The compounds showed no hemolytic activity. CONCLUSIONS The tested compounds from P. tuberculatum exhibited antiparasitic and cytotoxic effects.
DOI: 10.6084/m9.figshare.7452386
title: Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

description: Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.
DOI: 10.6084/m9.figshare.7452383.v1
title: Antiplasmodial and antileishmanial activities of compounds from Piper tuberculatum Jacq fruits

description: Abstract INTRODUCTION This study assessed the activity of compounds from Piper tuberculatum against Plasmodium falciparum and Leishmania guyanensis. METHODS The effects of compounds from P. tuberculatum fruits on P. falciparum and L. guyanensis promastigote growth in vitro were determined. Hemolytic action and cytotoxicity in HepG2 and J774 cells were measured. RESULTS Three compounds showed strong antiplasmodial activity and one compound showed strong antileishmanial activity. Two compounds were non-toxic to HepG2 cells and all were toxic to J774 cells. The compounds showed no hemolytic activity. CONCLUSIONS The tested compounds from P. tuberculatum exhibited antiparasitic and cytotoxic effects.