cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/41xas-as365
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-28/rawdata/</p>dH_LG_64step2_17.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-28/rawdata/dH_LG_64step2_17.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8bv7r-bfq30
title: JV9082

description: DotF-GFP, defocus range -3 to 6
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -5.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Strain from Joe Vogel lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Late exponential phase, early stationary phase</p>
<strong>Sample Preparation:</strong> 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-220/rawdata/</p>9082_box2_No3_107.mrc,  Tilt Series (Pixel Size 0.2612 nm), 188.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-12-05-220/rawdata/9082_box2_No3_107.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hc731-a1125
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-65/rawdata/</p>dH_LG_64step2_36.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-65/rawdata/dH_LG_64step2_36.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1xdnn-9m439
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-353/rawdata/</p>JV9298_PD-1-3_325.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-353/rawdata/JV9298_PD-1-3_325.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/scgst-nv223
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-390/rawdata/</p>JV9298_PD-1-3_374.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-390/rawdata/JV9298_PD-1-3_374.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7qa1r-mp731
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-53/rawdata/</p>dH_LG_64step2_27.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-53/rawdata/dH_LG_64step2_27.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m8j0b-x4b15
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-05-20</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, by PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-05-20-7/rawdata/</p>targ5.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-05-20-7/rawdata/targ5.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c5v5k-dc070
title: Legionella_delta-lspHIJK

description: Legionella pneumophila Philadelphia-1_Lp02. Lacks all the T2SS minor pseudopilins.
description: <strong>Tilt Series Date:</strong> 2019-05-16</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for this strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-42/rawdata/</p>DG7_20190516_tomo0021.mrc,  Tilt Series (Pixel Size 0.26 nm), 455.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-16-42/rawdata/DG7_20190516_tomo0021.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hfze2-yg289
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-17</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-93/rawdata/</p>targ91.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-17-93/rawdata/targ91.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rq7v3-cec51
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-102/rawdata/</p>JV9298_PD-1-3_469.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-102/rawdata/JV9298_PD-1-3_469.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/snn9v-1pj72
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-395/rawdata/</p>JV9298_PD-1-3_379.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-395/rawdata/JV9298_PD-1-3_379.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r0ndd-6gz47
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-36/rawdata/</p>AnchorLM.mrc,  Tilt Series (Pixel Size 0.39 nm), 369.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-36/rawdata/AnchorLM.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/98dk3-c3x92
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2018-10-05</p>
<strong>Data Taken By:</strong> Lujia Zhang</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 252.8 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> For the research of pilus, Del E from Partick</p>
<strong>Purification / Growth Conditions / Treatment:</strong> swarmer cell, PVP</p>
<strong>Sample Preparation:</strong> force 4, time 6, humidity 100%, temp. 22</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-29/rawdata/</p>targ29.mrc,  Tilt Series (Pixel Size 0.3712 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lza2018-10-05-29/rawdata/targ29.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p3tsc-f3103
title: Legionella delta DotO

description: Strain from Yale
description: <strong>Tilt Series Date:</strong> 2019-01-15</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 53000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Jun Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Early stationary phase, broad vitrobot, R2/2 quantifoil 200 mesh copper grid</p>
<strong>Sample Preparation:</strong> 10 nm gold beads</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-78/rawdata/</p>tilt_94.mrc,  Tilt Series (Pixel Size 0.27 nm), 313.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-01-15-78/rawdata/tilt_94.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ghtvj-3gz89
title: B. diazoefficiens, hopanoids

description: delta HpnH strain, log phase
description: <strong>Tilt Series Date:</strong> 2019-11-06</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Bradyrhizobium diazoefficiens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> Brittany Belin (Newman Lab, CalTech): strain construction, culture growth</p>
<strong>Purification / Growth Conditions / Treatment:</strong> log phase, treated with naringenin</p>
<strong>Sample Preparation:</strong> Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-77/rawdata/</p>dH_LG_64step2_48.mrc,  Tilt Series (Pixel Size 2.678 nm), 3.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-77/rawdata/dH_LG_64step2_48.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7sjks-5s416
title: Pseudomonas aeruginoas_JT2944

description: Raw data files of Pseudomonas aeruginoas_JT2944
description: <strong>Tilt Series Date:</strong> 2018-08-16</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> Cells produced by Jonida Toska from Melakanos lab.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown overnight in LB at 37 C.</p>
<strong>Sample Preparation:</strong> Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-16-99/rawdata/</p>Pseudomonas_JT2944Tilt524.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
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