cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/es6at-w4j94
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 20 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-52/rawdata/</p>Hpylori_DFliF_FISE_026.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fg3d0-p2760
title: P_aeruginosa_PA14_DfliF_Krios

description: Raw data files of P_aeruginosa_PA14_DfliF_Krios
description: <strong>Tilt Series Date:</strong> 2018-09-29</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (50.0°, -50.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> I (mohammed) obtained the cells from Shannon Park (Dianne Newman lab). The cells are available in their library of Pseudomonas aeruginosa PA14.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated one colony from an LB agar plate and grew the cells overnight in LB with 100 ug/ml of Gentamycin. Then I concentrated the cells three times and mixed them with gold nanoparticles and added 3 ul on to thick carbon R2 grids and blotted them and plunge froze them (force either 6 or 3, time either 3 or 2 seconds respectively, drain time 1 second).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-7/rawdata/</p>MontageSq4.mrc,  Tilt Series (Pixel Size 0.53 nm), 576.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-7/rawdata/MontageSq4.mrc"
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DOI: 10.22002/gvfp9-6s775
title: Shewanella MR1_Iron cores_Chemos

description: Raw data files of Shewanella MR1_Iron cores_Chemos
description: <strong>Tilt Series Date:</strong> 2018-08-09</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -9.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These are Shewanella oneidensis MR1 prepared by Sahand (USC) by Chemostat method grown for 2 days and chemically fixed. I (Mohammed) imaged these cells on the Polara to see if the have the iron cores in order to send them to Israel (Sharon Wolf) to perform EDX and confirm the components of the iron cores.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Chemostat 2 days growth and chemically fixed. The samples were prepared by Sahand (USC).</p>
<strong>Sample Preparation:</strong> I prepared these samples by blot force 6, blot time 3s and drain time 1. I also used the Bdellovibrio conditions (force -5, time 4 s). 10 nm Gold nanoparticles were added.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-35/rawdata/</p>SheweTilt15.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-08-09-35/rawdata/SheweTilt15.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v6z8r-2pw86
title: Exp phase S. acidocaldarius

description: Grid box 80 - grid1. Located dividing cells using projection images in a previous TEM session.
description: <strong>Tilt Series Date:</strong> 2018-01-28</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD for alignment and tomo3D for SIRT.</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were harvested at 0.06 OD and kept at 4 C for further steps until freezing. Spun cells at 6000 xg for 3 min to concentrated cells to ~4 OD and mixed with equal volume of 10 nm Au beads (40X concentrated in 0.01 % BSA) to reach final concentration of ~2 OD.</p>
<strong>Sample Preparation:</strong> Cu finder grids - glow discharged. Added 3.5 µL of suspension and blotted for 2-3 sec from behind manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-28-8/rawdata/</p>TS8-124.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-28-8/rawdata/TS8-124.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2998d-w1r31
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-351/rawdata/</p>Hpylori_DFliF_FISE_356.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.aefpmn
title: Occurrence Download

description: A dataset containing 887024 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Aves",
 "Year is greater than or equal to 1900",
 "Year is less than or equal to 1990",
 "HasCoordinate is true",
 "Geometry MULTIPOLYGON (((124 -15, 124 -17, 123 -17, 122 -17, 122 -19, 123 -19, 123 -18, 124 -18, 125 -18, 126 -18, 127 -18, 128 -18, 128 -17, 129 -17, 130 -17, 131 -17, 132 -17, 133 -17, 134 -17, 135 -17, 136 -17, 136 -18, 137 -18, 138 -18, 138 -19, 139 -19, 140 -19, 140 -16, 139 -16, 139 -17, 138 -17, 138 -16, 137 -16, 136 -16, 136 -13, 137 -13, 137 -12, 136 -12, 135 -12, 134 -12, 133 -12, 132 -12, 132 -11, 131 -11, 130 -11, 130 -12, 131 -12, 131 -13, 130 -13, 130 -14, 129 -14, 129 -15, 128 -15, 128 -14, 127 -14, 126 -14, 126 -15, 125 -15, 124 -15)), ((150 -32, 150 -35, 151 -35, 151 -33, 152 -33, 152 -32, 151 -32, 150 -32)), ((125 -10, 125 -9, 124 -9, 124 -10, 125 -10)), ((145 -17, 145 -14, 144 -14, 143 -14, 143 -11, 142 -11, 142 -15, 141 -15, 141 -20, 142 -20, 143 -20, 143 -21, 144 -21, 145 -21, 145 -22, 146 -22, 146 -23, 147 -23, 147 -24, 148 -24, 148 -25, 149 -25, 150 -25, 150 -26, 151 -26, 151 -30, 152 -30, 152 -32, 153 -32, 153 -28, 154 -28, 154 -27, 153 -27, 153 -25, 152 -25, 152 -24, 151 -24, 151 -23, 150 -23, 150 -22, 149 -22, 149 -21, 148 -21, 148 -20, 147 -20, 146 -20, 146 -19, 145 -19, 144 -19, 144 -17, 145 -17)))"
 ]
}

 The dataset includes 887024 records from 73 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006557-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.kkdjr7
title: Occurrence Download

description: A dataset containing 242 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Chuquiraga parviflora"
 ]
}

 The dataset includes 242 records from 25 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006610-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/vxsx3-kvp29
title: JV9082_K3_janelia

description: JV9082:DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2019-12-05</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -4.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-05-29/rawdata/</p>9082_Box2_grid3_33.mrc,  Tilt Series (Pixel Size 0.261 nm), 660.0 MB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fh3b8-as089
title: HeLa (Myo1a KD) - PM damaged

description: HeLa (Kyoto line stably expressing CHMP4B-EGFP at low levels;courtesy MPI) knocked down for Myo1a (double transfection) and laser damaged. Grid - WY46_grid1 and WY46_grid2 (TS1-19 from grid2 and TS20-42 from grid1)
description: <strong>Tilt Series Date:</strong> 2018-06-16</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD and tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, transfected with siRNA and caused plasma membrane damage using Laser before freezing. Shrawan performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were knocked down for Myo1a with SMART pool siRNA for 48 hr (2 x 24 hr). Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-06-16-15/rawdata/</p>TS17.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-06-16-15/rawdata/TS17.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t1m89-4ze78
title: Exp phase S. acidocaldarius

description: Grid box 80 - grid1. Located dividing cells using projection images in a previous TEM session.
description: <strong>Tilt Series Date:</strong> 2018-01-28</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD for alignment and tomo3D for SIRT.</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were harvested at 0.06 OD and kept at 4 C for further steps until freezing. Spun cells at 6000 xg for 3 min to concentrated cells to ~4 OD and mixed with equal volume of 10 nm Au beads (40X concentrated in 0.01 % BSA) to reach final concentration of ~2 OD.</p>
<strong>Sample Preparation:</strong> Cu finder grids - glow discharged. Added 3.5 µL of suspension and blotted for 2-3 sec from behind manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-28-7/rawdata/</p>TS7-117.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-28-7/rawdata/TS7-117.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gs19q-70140
title: Exp phase S. acidocaldarius

description: Grid box 80 - grid1. Located dividing cells using projection images in a previous TEM session.
description: <strong>Tilt Series Date:</strong> 2018-01-28</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD for alignment and tomo3D for SIRT.</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were harvested at 0.06 OD and kept at 4 C for further steps until freezing. Spun cells at 6000 xg for 3 min to concentrated cells to ~4 OD and mixed with equal volume of 10 nm Au beads (40X concentrated in 0.01 % BSA) to reach final concentration of ~2 OD.</p>
<strong>Sample Preparation:</strong> Cu finder grids - glow discharged. Added 3.5 µL of suspension and blotted for 2-3 sec from behind manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-28-6/rawdata/</p>TS6-115.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-01-28-6/rawdata/TS6-115.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.6wnr92
title: Occurrence Download

description: A dataset containing 182 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (China, Mongolia)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Catolobus pendulus"
 ]
}

 The dataset includes 182 records from 18 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006609-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/m820x-hqc38
title: HeLa (Myo1a KD) - PM damaged

description: HeLa (Kyoto line stably expressing CHMP4B-EGFP at low levels;courtesy MPI) knocked down for Myo1a (double transfection) and laser damaged. Grid - WY46_grid1 and WY46_grid2 (TS1-19 from grid2 and TS20-42 from grid1)
description: <strong>Tilt Series Date:</strong> 2018-06-16</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD and tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, transfected with siRNA and caused plasma membrane damage using Laser before freezing. Shrawan performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were knocked down for Myo1a with SMART pool siRNA for 48 hr (2 x 24 hr). Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-06-16-13/rawdata/</p>TS15.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
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        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/krrzb-s2x20
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-285/rawdata/</p>Hpylori_DFliF_FISE_280.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.8 GB  
        
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