DOI: 10.5061/dryad.92j4913
title: Data from: Genetic structure and diversity of the blueface darter Etheostoma cyanoprosopum, a microendemic freshwater fish in the southeastern United States

description: Darters represent one of the most diverse groups of freshwater fishes in
 North America, but approximately 33% of the 215 recognized species are
 considered imperiled on the IUCN Red List. Discovery and description of
 new darter species continues at a relatively rapid pace, with many
 exhibiting microendemic geographic distributions and little baseline data
 for conservation decisions. The Blueface Darter Etheostoma cyanoprosopum
 is a newly described species that occupies &lt; 20 km of stream
 reaches in the Bear Creek system of the Tennessee River drainage and the
 Hubbard Creek system of the Black Warrior River drainage in the Mobile
 Basin (Alabama, USA). In addition to restricted distribution, the species
 is threatened by habitat degradation and several natural and man-made
 barriers that putatively fragment connectivity among populations. This
 study used microsatellite and mitochondrial (mt) DNA data with comparisons
 to the relatively broadly distributed sister species (Bandfin Darter E.
 zonistium) to evaluate multiple objectives about genetic connectivity and
 diversity among Blueface Darter populations. Analysis of mtDNA data
 indicated a lack of historical structuring across the Tennessee-Black
 Warrior river drainage divide and within the Bear Creek system. However,
 microsatellite-based Bayesian cluster analyses and F-statistics suggested
 contemporary isolation across the drainage divide and evidence for
 reservoir-induced fragmentation within the Bear Creek system. Compared to
 the sister Bandfin Darter, Blueface Darter populations exhibited reduced
 levels of genetic diversity, rendering them more susceptible to local
 extirpation and reduced fitness. Continued monitoring and quantitative
 ecological studies are recommended to understand population-specific
 measures of occurrence and abundance.
description: Microsatellite data
 GENEPOP formatThe microsatellite DNA
 data file is in GENEPOP format. The file includes genotype scores for
 eight microsatellite loci for Blueface (cfzon) and Bandfin (zon) darters.
 Locus names are listed under the title line at the top of the file. POP
 refers to populations described in the manuscript. The populations are
 ordered as follows: (Blueface Darter) 1. Little Bear Creek, 2. Turkey
 Creek, 3. Bear Creek, 4. Hubbard Cr. above falls, 5. Hubbard Cr. below
 falls; (Bandfin Darter) 6. Bear Creek, 7. Big Sandy River, 8. Birdsong
 Creek, and 9. Hatchie River. Each row below POP identifiers represents an
 individual followed by their genotype for the eight loci. Individual
 identifiers and specimen information can be found in the associated excel
 file named "Blueface_Bandfin_specimen_info.xlsx". Allele scores
 for each locus are concatenated into six digits, with the first three
 numbers representing the size of allele "one" and the second
 three numbers representing the size of allele "two". Missing
 data are coded as "000".Blueface_Bandfin_GENEPOP.txtmtDNA cytochrome b sequence alignmentThe DNA sequence file is in FASTA format. The file includes 18 aligned mtDNA cytochrome b sequences generated from Blueface Darter samples used in this study (samples with “cfzon” identifiers). Individual identifiers and specimen information can be found in the associated excel file named "Blueface_Bandfin_specimen_info.xlsx". The file includes an additional 16 cyt b sequences of Blueface Darter (samples with “Ebfd” identifiers) obtained from Kozal LC, Simmons JW, Mollish JM, MacGuigan DJ, Benavides E, Keck BP, Near TJ (2017) Data from: Phylogenetic and morphological diversity of the Etheostoma zonistium species complex with the description of a new species endemic to the Cumberland Plateau of Alabama. Dryad Digital Repository. https://doi.org/10.5061/dryad.s7h6bBlueface_cytb_alignment.fstBlueface_Bandfin_specimen_infoDetailed information for Blueface and Bandfin darter samples used in this study. Museum tissue catalog or specimen voucher catalog numbers are followed by individual identifiers and collection locality information. Specimens and/or tissues were cataloged in the Arkansas State University Museum of Zoology (ASUMZ) or the University of Alabama Ichthyological Collection (UAIC).