cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19901070.v1
title: Physiological and biochemical changes in ‘Fuyu’ persimmon buds during dormancy

description: ABSTRACT: This study identified physiological and biochemical changes in ‘Fuyu’ persimmon buds during dormancy. Branches were collected between March and August 2015. Dormancy was evaluated by biological testing of isolated node cuttings at 25 °C and a photoperiod of 16 h. The variables analyzed were water content; reducing sugar content; respiratory activity; activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (POD) and polyphenol oxidase (PPO) enzymes; hydrogen peroxide content (H2O2) and lipid peroxidation. At the end of March 2015, the plants were already dormant, and the leaves and fruits present indicated a paradormancy effect. Induction of endodormancy may have occurred in June 2015, when chilling hours (CH) below 7.2 °C and higher CH below 12 °C began to accumulate, which coincided with the period in which there was a decrease in water content and respiratory activity, an increase in reducing sugars, a decrease in SOD, CAT, APX and PPO and an increase in H2O2. After an accumulation of 553 CH below 12 °C, the budburst capacity increased, and the buds presented increased water content, decreased reducing sugars content, increased respiratory activity, low activity in SOD, CAT, APX and POD and high levels of H2O2.
DOI: 10.6084/m9.figshare.19901086
title: Physical Exercise Training and Chagas Disease: Potential Role of MicroRNAs

description: Abstract Chagas disease (CD) is caused by Trypanosoma Cruzi. This parasite can infect several organs of the human body, mainly the heart, causing inflammation, fibrosis, arrhythmias, and cardiac remodeling, promoting long-term Chronic Chagas Cardiomyopathy (CCC). However, little scientific evidence has elucidated the molecular mechanisms that govern the pathophysiological processes in this disease. MicroRNAs (miRNAs) are regulators of post-transcriptional gene expression that modulate signaling pathways, participating in pathophysiological mechanisms in CD, but the understanding of miRNAs in this disease is limited. On the other hand, a wide range of scientific evidence shows that physical exercise training (PET) modulates the expression of miRNAs by modifying different signaling pathways in healthy individuals. Some studies also show that PET is beneficial for individuals with CD; however, these did not evaluate the miRNA expressions. Thus, there is no evidence showing the role of PET in the expression of miRNAs in CD. Therefore, this review aimed to identify miRNAs expressed in CD that could potentially be modified by PET.
DOI: 10.6084/m9.figshare.19901086.v1
title: Physical Exercise Training and Chagas Disease: Potential Role of MicroRNAs

description: Abstract Chagas disease (CD) is caused by Trypanosoma Cruzi. This parasite can infect several organs of the human body, mainly the heart, causing inflammation, fibrosis, arrhythmias, and cardiac remodeling, promoting long-term Chronic Chagas Cardiomyopathy (CCC). However, little scientific evidence has elucidated the molecular mechanisms that govern the pathophysiological processes in this disease. MicroRNAs (miRNAs) are regulators of post-transcriptional gene expression that modulate signaling pathways, participating in pathophysiological mechanisms in CD, but the understanding of miRNAs in this disease is limited. On the other hand, a wide range of scientific evidence shows that physical exercise training (PET) modulates the expression of miRNAs by modifying different signaling pathways in healthy individuals. Some studies also show that PET is beneficial for individuals with CD; however, these did not evaluate the miRNA expressions. Thus, there is no evidence showing the role of PET in the expression of miRNAs in CD. Therefore, this review aimed to identify miRNAs expressed in CD that could potentially be modified by PET.
DOI: 10.6084/m9.figshare.19901098
title: SEASONALITY OF THE BARK TANNINS CONTENT OF FIVE-YEAR-OLD Acacia mangium TREES GROWN IN NORTHEAST BRAZIL

description: ABSTRACT This study aimed to assess the influence of the seasonality on the bark tannins content of Acacia mangium trees grown in the Northeastern Brazilian Region and the effect of soil preparation on the results. Two experimental plots of 1.0 ha each were submitted to different soil preparation methods, with two different intensities. The experimental design consisted of four treatments, two types of soil preparation and, two different bark collection seasons (end of the rainy and dry seasons). The bark of the trees was collected in each treatment and the contents of condensed tannins were determined. For each experimental treatment, 15 trees were harvested and debarked. Bark material was submitted to extraction with hot water, obtaining the total solids content (TSC), Stiasny index (I), and the condensed tannins content (CTC). There was no influence of the soil preparation method on the TSC, I, and CTC. However, there was a significant difference in these parameters for tree bark collected in the rainy season, with higher values. The less intensive soil preparation method is recommended due to its lower cost, and bark should be collected at the end of the rainy season for the best yield of condensed tannins.
DOI: 10.6084/m9.figshare.19901098.v1
title: SEASONALITY OF THE BARK TANNINS CONTENT OF FIVE-YEAR-OLD Acacia mangium TREES GROWN IN NORTHEAST BRAZIL

description: ABSTRACT This study aimed to assess the influence of the seasonality on the bark tannins content of Acacia mangium trees grown in the Northeastern Brazilian Region and the effect of soil preparation on the results. Two experimental plots of 1.0 ha each were submitted to different soil preparation methods, with two different intensities. The experimental design consisted of four treatments, two types of soil preparation and, two different bark collection seasons (end of the rainy and dry seasons). The bark of the trees was collected in each treatment and the contents of condensed tannins were determined. For each experimental treatment, 15 trees were harvested and debarked. Bark material was submitted to extraction with hot water, obtaining the total solids content (TSC), Stiasny index (I), and the condensed tannins content (CTC). There was no influence of the soil preparation method on the TSC, I, and CTC. However, there was a significant difference in these parameters for tree bark collected in the rainy season, with higher values. The less intensive soil preparation method is recommended due to its lower cost, and bark should be collected at the end of the rainy season for the best yield of condensed tannins.
DOI: 10.6084/m9.figshare.19901106
title: Physiological Quality of Stored Dipteryx alata Vogel Seeds: Tetrazolium Test and Radiographic Imaging Standardization

description: Abstract The baru (Dipteryx alata Vogel), is fruit tree displaying economic, environmental, industrial and food importance. The objective of this study was to adapt to the tetrazolium test methodology to baru seeds and evaluate the physiological quality of seeds stored for 12 months at 10 and 20 ºC, using X-ray images as a toll for detection damage throughout the storage period while also carrying out germination, emergence and electrical conductivity tests. Baru seeds remained viable for 12 months of storage, albeit with a small decrease in vigor after nine months. The applied tetrazolium test methodology was efficient in determining internal seed damage. The pre-soak time for the tetrazolium test and solution concentration was 24 hours and 0.05%. No changes in seed morphology and radiographic images were observed during the storage periods, regardless of the assessed temperature. The X-ray test enabled a precise visualization of internal baru seed structures.
DOI: 10.6084/m9.figshare.19901106.v1
title: Physiological Quality of Stored Dipteryx alata Vogel Seeds: Tetrazolium Test and Radiographic Imaging Standardization

description: Abstract The baru (Dipteryx alata Vogel), is fruit tree displaying economic, environmental, industrial and food importance. The objective of this study was to adapt to the tetrazolium test methodology to baru seeds and evaluate the physiological quality of seeds stored for 12 months at 10 and 20 ºC, using X-ray images as a toll for detection damage throughout the storage period while also carrying out germination, emergence and electrical conductivity tests. Baru seeds remained viable for 12 months of storage, albeit with a small decrease in vigor after nine months. The applied tetrazolium test methodology was efficient in determining internal seed damage. The pre-soak time for the tetrazolium test and solution concentration was 24 hours and 0.05%. No changes in seed morphology and radiographic images were observed during the storage periods, regardless of the assessed temperature. The X-ray test enabled a precise visualization of internal baru seed structures.
DOI: 10.6084/m9.figshare.19901110
title: MORPHOLOGICAL DESCRIPTORS FOR THE CHARACTERISATION OF TEAK CLONES CUTTINGS (Tectona grandis L.F.)

description: ABSTRACT Teak (Tectona grandis) is a forest species cultivated in tropical regions worldwide, due to the traits of its wood and its great economic value on the market. In Brazil, original plantations are being replaced by selected clonal forests, which generate the need and interest to protect this genetic material based on the current legislation. The legal requirements for cultivar protection are that the genotype must result from genetic improvement and provide distinguishability, homogeneity, and stability (DHS). This work identifies morphological descriptors in teak cuttings to assist the clone protection process of the species. The study was conducted in a completely randomized design with the evaluation of 7 clones and 15 cuttings per clone, at 30, 60, 90, and 120 days old. The information was also organized based on the arrays of presence and absence of variations for each clone, for 21 morphological traits. Subsequently, genetic similarity measures were estimated using the Jaccard index and the UPGMA clustering method. The clones with the 12, 7, 9, and 11 morphological traits formed the morphological descriptors for the 30, 60, 90, and 120 days old cuttings, respectively. The traits of the leaf blade: length, width, length/width ratio, green intensity, and the brightness of the leaf, were common for all the evaluated ages, but their respective levels of expression were different.
DOI: 10.6084/m9.figshare.19901110.v1
title: MORPHOLOGICAL DESCRIPTORS FOR THE CHARACTERISATION OF TEAK CLONES CUTTINGS (Tectona grandis L.F.)

description: ABSTRACT Teak (Tectona grandis) is a forest species cultivated in tropical regions worldwide, due to the traits of its wood and its great economic value on the market. In Brazil, original plantations are being replaced by selected clonal forests, which generate the need and interest to protect this genetic material based on the current legislation. The legal requirements for cultivar protection are that the genotype must result from genetic improvement and provide distinguishability, homogeneity, and stability (DHS). This work identifies morphological descriptors in teak cuttings to assist the clone protection process of the species. The study was conducted in a completely randomized design with the evaluation of 7 clones and 15 cuttings per clone, at 30, 60, 90, and 120 days old. The information was also organized based on the arrays of presence and absence of variations for each clone, for 21 morphological traits. Subsequently, genetic similarity measures were estimated using the Jaccard index and the UPGMA clustering method. The clones with the 12, 7, 9, and 11 morphological traits formed the morphological descriptors for the 30, 60, 90, and 120 days old cuttings, respectively. The traits of the leaf blade: length, width, length/width ratio, green intensity, and the brightness of the leaf, were common for all the evaluated ages, but their respective levels of expression were different.
DOI: 10.6084/m9.figshare.19901118
title: New sensitive real-time PCR targeting p28 gene for detection of Ehrlichia canis in blood samples from dogs

description: ABSTRACT: This study aims to describe a new detection method of a quantitative real-time polymerase chain reaction (qPCR) targeting the 28 kDa outer membrane protein gene (p28) as well as to compare this method with a conventional PCR (cPCR), which targets the same gene, in order to evaluate the performance of the technique designed in this study in detecting Ehrlichia canis (E. canis). Optimum oligonucleotides concentrations were reached, and the analytical sensitivity and specificity of the qPCR were performed. A total of 218 dogs’ whole blood samples were conventionally collected for this study. The DNA was extracted from each sample. Subsequently, the samples were tested by an established cPCR and the new qPCR to compare each technique’s performances. This new qPCR method for the molecular detection of E. canis presented a detection limit of ten copies of the fragment and was considered specific for E. canis according to analytical specificity analyses performed in vitro and in silico. The standard curve revealed 100% efficiency and a coefficient of determination (R2) equivalent to 99.8%. Among the samples examined by qPCR, 24.31% were considered positive, significantly greater than those detected by cPCR (15.13%). The qPCR technique reached a higher sensitivity than the cPCR when targeting the p28 gene in detecting E. canis. The qPCR standardized in this study is an efficient method for confirming canine monocytic ehrlichiosis (CME) diagnosis and might provide the parasitemia monitoring during the disease treatment.
DOI: 10.6084/m9.figshare.19901118.v1
title: New sensitive real-time PCR targeting p28 gene for detection of Ehrlichia canis in blood samples from dogs

description: ABSTRACT: This study aims to describe a new detection method of a quantitative real-time polymerase chain reaction (qPCR) targeting the 28 kDa outer membrane protein gene (p28) as well as to compare this method with a conventional PCR (cPCR), which targets the same gene, in order to evaluate the performance of the technique designed in this study in detecting Ehrlichia canis (E. canis). Optimum oligonucleotides concentrations were reached, and the analytical sensitivity and specificity of the qPCR were performed. A total of 218 dogs’ whole blood samples were conventionally collected for this study. The DNA was extracted from each sample. Subsequently, the samples were tested by an established cPCR and the new qPCR to compare each technique’s performances. This new qPCR method for the molecular detection of E. canis presented a detection limit of ten copies of the fragment and was considered specific for E. canis according to analytical specificity analyses performed in vitro and in silico. The standard curve revealed 100% efficiency and a coefficient of determination (R2) equivalent to 99.8%. Among the samples examined by qPCR, 24.31% were considered positive, significantly greater than those detected by cPCR (15.13%). The qPCR technique reached a higher sensitivity than the cPCR when targeting the p28 gene in detecting E. canis. The qPCR standardized in this study is an efficient method for confirming canine monocytic ehrlichiosis (CME) diagnosis and might provide the parasitemia monitoring during the disease treatment.
DOI: 10.6084/m9.figshare.19901122
title: Typical performance behavior of a Diesel cycle agricultural tractor engine with electronic injection management and turbocharger

description: ABSTRACT: Over the past few years, changes have been observed in the behavior of typical performance curves, after higher technology has been incorporated into the agricultural tractor engines, either to satisfy the ceiling of the new pollutant gas emission limits or to achieve better efficiency. The aim of this study was to assess the typical performance behavior of an agricultural tractor engine, possessing electronic injection management, turbocharger, aftercooler and EGR (Exhaust Gas Recirculation) treatment system. An analysis was done of the information the manufacturers provided, in terms of nominal power, maximum power and maximum power with Booster, maximum torque and maximum torque with Booster. From the results, the percentage difference between the nominal power and maximum power were found to hover anywhere from 1.49 to 11.97%, for the same manufacturer, 0% in another manufacturer and 9% in a third manufacturer, for all the models. Similar results were noted for the data reported on the maximum torque and maximum torque with Booster, giving 0.62% as the minimum value, 7.44% as the average value and 12.65% as the maximum value for the entire series.
DOI: 10.6084/m9.figshare.19901122.v1
title: Typical performance behavior of a Diesel cycle agricultural tractor engine with electronic injection management and turbocharger

description: ABSTRACT: Over the past few years, changes have been observed in the behavior of typical performance curves, after higher technology has been incorporated into the agricultural tractor engines, either to satisfy the ceiling of the new pollutant gas emission limits or to achieve better efficiency. The aim of this study was to assess the typical performance behavior of an agricultural tractor engine, possessing electronic injection management, turbocharger, aftercooler and EGR (Exhaust Gas Recirculation) treatment system. An analysis was done of the information the manufacturers provided, in terms of nominal power, maximum power and maximum power with Booster, maximum torque and maximum torque with Booster. From the results, the percentage difference between the nominal power and maximum power were found to hover anywhere from 1.49 to 11.97%, for the same manufacturer, 0% in another manufacturer and 9% in a third manufacturer, for all the models. Similar results were noted for the data reported on the maximum torque and maximum torque with Booster, giving 0.62% as the minimum value, 7.44% as the average value and 12.65% as the maximum value for the entire series.
DOI: 10.6084/m9.figshare.19901143
title: Influence of Geographical Location on Access to Reperfusion Therapies and Mortality of Patients with STEMI in Sergipe: VICTIM Register

description: Abstract Background The concentration of high-complexity services in Aracaju, Sergipe can impose certain disparity in the quality of care for the patients with ST-segment elevation acute myocardial infarction (STEMI) (STEMI) who receive care from Brazil’s Unified Health System (SUS, acronym in Portuguese) and whose symptoms started in other health regions of the state. Objective To evaluate disparities in access to reperfusion therapies and 30-day mortality, among patients with STEMI, who were users of SUS, in each of the 7 health regions of Sergipe. Methods A total of 844 patients with STEMI in the period from 2014 to 2018, assisted by the only hospital with the capacity to offer primary percutaneous coronary intervention (PPCI) to SUS users in the state of Sergipe, were evaluated. The patients were divided into 7 groups according to the location at the onset of symptoms, following the existing division of health regions in the state. For comparison between groups, a significant difference was considered when p &lt; 0.05. Results Of the total of 844 patients suffering from STEMI who were transferred to the hospital with PPCI that serves SUS patients, 386 patients (45.8%) underwent primary angioplasty. The mean rate of fibrinolytic use was 2.6%, with no differences between the regions. The mean total time of arrival to the hospital with PPCI was 21 hours and 55 minutes, with a median of 10 hours and 22 minutes (6 hours and 30 minutes to 22 hours and 52 minutes). Total 30-day mortality was 12.8%, but without differences between the regions, even when adjusted for age and sex. Conclusions This study reveals that fibrinolytics are underused throughout the state and that there is a significant delay in access to the hospital with PPCI, in all health regions of Sergipe.
DOI: 10.6084/m9.figshare.19901143.v1
title: Influence of Geographical Location on Access to Reperfusion Therapies and Mortality of Patients with STEMI in Sergipe: VICTIM Register

description: Abstract Background The concentration of high-complexity services in Aracaju, Sergipe can impose certain disparity in the quality of care for the patients with ST-segment elevation acute myocardial infarction (STEMI) (STEMI) who receive care from Brazil’s Unified Health System (SUS, acronym in Portuguese) and whose symptoms started in other health regions of the state. Objective To evaluate disparities in access to reperfusion therapies and 30-day mortality, among patients with STEMI, who were users of SUS, in each of the 7 health regions of Sergipe. Methods A total of 844 patients with STEMI in the period from 2014 to 2018, assisted by the only hospital with the capacity to offer primary percutaneous coronary intervention (PPCI) to SUS users in the state of Sergipe, were evaluated. The patients were divided into 7 groups according to the location at the onset of symptoms, following the existing division of health regions in the state. For comparison between groups, a significant difference was considered when p &lt; 0.05. Results Of the total of 844 patients suffering from STEMI who were transferred to the hospital with PPCI that serves SUS patients, 386 patients (45.8%) underwent primary angioplasty. The mean rate of fibrinolytic use was 2.6%, with no differences between the regions. The mean total time of arrival to the hospital with PPCI was 21 hours and 55 minutes, with a median of 10 hours and 22 minutes (6 hours and 30 minutes to 22 hours and 52 minutes). Total 30-day mortality was 12.8%, but without differences between the regions, even when adjusted for age and sex. Conclusions This study reveals that fibrinolytics are underused throughout the state and that there is a significant delay in access to the hospital with PPCI, in all health regions of Sergipe.
DOI: 10.6084/m9.figshare.19901161
title: Podding of Paralomis granulosa (Lithodidae) juveniles inhabiting kelp forests of the Cape Horn Archipelago (Chile)

description: Abstract Subtidal observations along the Cape Horn Archipelago, Chile (CHA) in February 2017 revealed an unusually large aggregation (or pod) of juvenile false king crabs, Paralomis granulosa (Hombron and Jacquinot, 1846), in association with kelp forests (Macrocystis pyrifera and Lessonia spp.). This is the first study to report a dense aggregation of juveniles of this crab, which was observed at Wollaston Island (WI) (~ 10 m). Paralomis granulosa was present on half the transects at WI (N=10), with a density of 3.1 ± 9.9 ind. m-2. Photographs from the podding event showed densities of P. granulosa ranging from 63 to 367 ind. plant-1 (190 ± 133 ind. plant-1). Juveniles (32.8 ± 7.3 mm carapace length) were recorded on kelp fronds, holdfasts, kelp stipes, and adjacent rocky bottom of this protected coast. This podding behavior resembles that of other juvenile king crabs in terms of homogeneity in size structure and may be a predator avoidance mechanism. These observations highlight three aspects of this kelp-animal relationship: (i) identification of a previously unknown ecosystem service provided by sub-Antarctic kelp forests to the associated benthic fauna; (ii) the ecological value of kelp as a bioengineering species; and (iii) pods being an important attribute for population assessments. Due to the importance of the CHA in the life cycle for this and other species, we suggest the archipelago be incorporated within the recently established Diego Ramírez Island-Drake Passage Marine Park.