DOI: 10.5061/dryad.39vb6
title: Data from: Model-assisted analysis of sugar metabolism throughout tomato fruit development reveals enzyme and carrier properties in relation to vacuole expansion

description: A kinetic model combining enzyme activity measurements and subcellular
 compartmentation was parameterized to fit the sucrose, hexose, and
 glucose-6-P contents of pericarp throughout tomato (Solanum lycopersicum)
 fruit development. The model was further validated using independent data
 obtained from domesticated and wild tomato species and on transgenic
 lines. A hierarchical clustering analysis of the calculated fluxes and
 enzyme capacities together revealed stage-dependent features. Cell
 division was characterized by a high sucrolytic activity of the vacuole,
 whereas sucrose cleavage during expansion was sustained by both sucrose
 synthase and neutral invertase, associated with minimal futile cycling.
 Most importantly, a tight correlation between flux rate and enzyme
 capacity was found for fructokinase and PPi-dependent phosphofructokinase
 during cell division and for sucrose synthase, UDP-glucopyrophosphorylase,
 and phosphoglucomutase during expansion, thus suggesting an adaptation of
 enzyme abundance to metabolic needs. In contrast, for most enzymes, flux
 rates varied irrespectively of enzyme capacities, and most enzymes
 functioned at &lt;5% of their maximal catalytic capacity. One of the
 major findings with the model was the high accumulation of soluble sugars
 within the vacuole together with organic acids, thus enabling the
 osmotic-driven vacuole expansion that was found during cell division.
description: Beauvoit et al._tomato
 fruit sugar model_high affinity_4 dpaCOPASI file of kinetic
 model of 4 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato fruit
 sugar model_high
 affinity_4dpa.cpsBeauvoit et al._tomato
 fruit sugar model_high affinity_8dpaCOPASI file of kinetic
 model of 8 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_15dpaCOPASI file of kinetic
 model of 15 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_22dpaCOPASI file of kinetic
 model of 22 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_27dpaCOPASI file of kinetic
 model of 27 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_30 dpaCOPASI file of kinetic
 model of 30 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_35dpaCOPASI file of kinetic
 model of 35 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_40dpaCOPASI file of kinetic
 model of 40 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_44 dpaCOPASI file of kinetic
 model of 44 DPA-aged fruit parameterized with high affinity vacuolar
 carriersBeauvoit et al._tomato
 fruit sugar model_high affinity_47 dpaCOPASI file of kinetic
 model of 47 DPA-aged fruit parameterized with high affinity vacuolar
 carriers