DOI: 10.22002/83ta6-me340
title: Xanthomonas WT

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9)
description: <strong>Tilt Series Date:</strong> 2017-03-03</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the strain, data collected by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in 2xTY with apt antibiotics, freeze cells next day after about 14 hour when they are in stationary phase.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 5 sec. ** Use 7 sec for next sessions.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-03-14/rawdata/</p>Xac_wt_20170303b10018.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-03-14/rawdata/Xac_wt_20170303b10018.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_wt_20170303b10018_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-03-14/3dimage_109094/Xac_wt_20170303b10018_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-03-03-14_slicer19203.png,  I suspect this is a REZ (although I admit it is very difficult to resolve. there are a few ribosomes visible in the center of the cell.  some other ribosomes may be present but the contrast is too poor to see them)  -andrew, 945.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-03-03-14_slicer19203.png"
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        Xac_wt_20170303b10018.ali,  327.9 MB  
        
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        Xac_wt_20170303b10018.tlt,  368.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-03-14/file_25710/Xac_wt_20170303b10018.tlt"
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        Xac_wt_20170303b10018_fid.tlt,  368.0 B  
        
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