DOI: 10.5061/dryad.6pr21
title: Data from: Validation of perfusion quantification with 3D gradient echo dynamic contrast-enhanced magnetic resonance imaging using a blood pool contrast agent in skeletal swine muscle

description: The purpose of our study was to validate perfusion quantification in a
 low-perfused tissue by dynamic contrast-enhanced magnetic resonance
 imaging (DCE-MRI) with shared k-space sampling using a blood pool contrast
 agent. Perfusion measurements were performed in a total of seven female
 pigs. An ultrasonic Doppler probe was attached to the right femoral artery
 to determine total flow in the hind leg musculature. The femoral artery
 was catheterized for continuous local administration of adenosine to
 increase blood flow up to four times the baseline level. Three different
 stable perfusion levels were induced. The MR protocol included a 3D
 gradient-echo sequence with a temporal resolution of approximately 1.5
 seconds. Before each dynamic sequence, static MR images were acquired with
 flip angles of 5°, 10°, 20°, and 30°. Both static and dynamic images were
 used to generate relaxation rate and baseline magnetization maps with a
 flip angle method. 0.1 mL/kg body weight of blood pool contrast medium was
 injected via a central venous catheter at a flow rate of 5 mL/s. The right
 hind leg was segmented in 3D into medial, cranial, lateral, and pelvic
 thigh muscles, lower leg, bones, skin, and fat. The arterial input
 function (AIF) was measured in the aorta. Perfusion of the different
 anatomic regions was calculated using a one- and a two-compartment model
 with delay- and dispersion-corrected AIFs. The F-test for model comparison
 was used to decide whether to use the results of the one- or
 two-compartment model fit. Total flow was calculated by integrating
 volume-weighted perfusion values over the whole measured region. The
 resulting values of delay, dispersion, blood volume, mean transit time,
 and flow were all in physiologically and physically reasonable ranges. In
 107 of 160 ROIs, the blood signal was separated, using a two-compartment
 model, into a capillary and an arteriolar signal contribution, decided by
 the F-test. Overall flow in hind leg muscles, as measured by the
 ultrasound probe, highly correlated with total flow determined by MRI, R =
 0.89 and P = 10−7. Linear regression yielded a slope of 1.2 and a y-axis
 intercept of 259 mL/min. The mean total volume of the investigated muscle
 tissue corresponds to an offset perfusion of 4.7mL/(min ⋅ 100cm3). The
 DCE-MRI technique presented here uses a blood pool contrast medium in
 combination with a two-compartment tracer kinetic model and allows
 absolute quantification of low-perfused non-cerebral organs such as
 muscles.
description: Experimental MR Signal
 Contrast Dynamics Magnitude Data Set 1MR Signal Magnitude
 Image Data Contrast Dynamics Experiment No. 1SE9.zipExperimental MR Signal Contrast Dynamics Magnitude Data Set 2MR Signal Magnitude Image Data Contrast Dynamics Experiment No. 2SE12.zipExperimental MR Signal Contrast Dynamics Magnitude Data Set 3MR Signal Magnitude Image Data Contrast Dynamics Experiment No. 3SE14.zipExperimental MR Signal Contrast Dynamics Magnitude Data Set 4MR Signal Magnitude Image Data Contrast Dynamics Experiment No. 4SE15.zipExperimental MR Signal Contrast Dynamics Magnitude Data Set 5MR Signal Magnitude Image Data Contrast Dynamics Experiment No. 5SE18.zipExperimental MR Signal Contrast Dynamics Magnitude Data Set 6MR Signal Magnitude Image Data Contrast Dynamics Experiment No. 6SE19.zipExperimental MR Signal Contrast Dynamics Magnitude Data Set 7MR Signal Magnitude Image Data Contrast Dynamics Experiment No. 7SE20.zip