DOI: 10.5061/dryad.8931zcs21
title: Kisspeptin photometry recordings during pregnancy and lactation

description: The specific role that prolactin plays in lactational infertility, as
 distinct from other suckling or metabolic cues, remains unresolved. Here,
 deletion of the prolactin receptor (Prlr) from forebrain neurons or
 arcuate kisspeptin neurons resulted infailure to maintain normal
 lactation-induced suppression of estrous cycles. Kisspeptin
 immunoreactivity and pulsatile LH secretion were increased in these mice,
 even in the presence of ongoing suckling stimulation and lactation. GCaMP
 fibre photometry of arcuate kisspeptin neurons revealed that the normal
 episodic activity of these neurons is rapidly suppressed in pregnancy and
 this was maintained throughout early lactation. Deletion of Prlr from
 arcuate kisspeptin neurons resulted in early reactivation of episodic
 activity of kisspeptin neurons prior to a premature return of reproductive
 cycles in early lactation. These observations show dynamic variation in
 arcuate kisspeptin neuronal activity associated with the hormonal changes
 of pregnancy and lactation, and provide direct evidence that prolactin
 action on arcuate kisspeptin neurons is necessary for suppressing
 fertility during lactation in mice.
description: GCaMP fibre photometry recordings of arcuate nucleus kisspeptin
 neurons throughout pregnancy and lactation. 
 Fluorescence signals were acquired using a custom-built fibre
 photometry system made primarily from Doric components. Violet (405nm) and
 blue (490nm) fibre-coupled LEDs were sinusoidally modulated at 531 and 211
 Hz, respectively, and focused into a 400μm, 0.48 numerical aperture fibre
 optic patch cord connected to the mouse. Emitted fluorescence was
 collected by the same fibre and focused onto a femtowatt photoreceiver
 (2151, Newport). The two GCaMP6s emission signals were collected at 10 Hz
 in a scheduled 5s on/15s off mode by demodulating the 405nm (non-calcium
 dependent) and 490nm (calcium-dependent) signals. The power output at the
 tip of the fibre was set at 50μW. Fluorescent signals were acquired using
 a custom software acquisition system (Tussock Innovation, Dunedin, New
 Zealand) and analyzed using custom templates created by Dr Joon Kim
 (University of Otago, Dunedin, New Zealand) based on mathematics and
 calculations similar to those previously described (see references in
 manuscript). Briefly, the fluorescent signal obtained after stimulation
 with 405nm light was used to correct for movement artefacts as follows:
 first, the 405nm signal was filtered using a savitzky-golay filter and
 fitted to the 490nm signal using least linear square regression. The
 fitted 405nm signal was then subtracted and divided from the 490nm signal
 to obtain the movement and bleaching corrected signal. The output of these
 templates is 490-adjusted405/adjusted405, which was multiplied to get the
 final ΔF/F as a percentage increase. All photometry data is reported as
 ΔF/F(%) which is given as Î”F/F(%) in the data files.
description: # Kisspeptin photometry during pregnancy and lactation
 [https://doi.org/10.5061/dryad.8931zcs21](https://doi.org/10.5061/dryad.8931zcs21) ## Description of the data and file structure These files contain the data used to construct the figures in the associated published manuscript in eLife. Data files contain processed data from GCaMP fiber photometry recordings (in .csv format), and in some cases plasma LH (luteinizing hormone) concentrations.  The raw data have been processed as described in the manuscript, and are provided here as ΔF/F(%) against time. Plasma LH concentrations are available at a limited number of time points and are provided as ng/ml. Each filename starts with a code number (F##) which is a unique identifier for each animal in the study (e.g. female number ##).  In most cases, there are multiple recordings from an individual animal collected at different times across pregnancy and lactation.  These are provided as separate files and are sequentially numbered with a full description in the file name (NL = non-pregnant/non-lactating). Data is provided as CVS files showing raw data, with each file representing data from individual animal (labelled F##), with repeated recordings form each animal labelled according to time during pregnancy or lactation.  The data specifically allign to figures presented in the paper. ## Access information Other publicly accessible locations of the data: * Please contact the authors if you would like the data in any other format Data was derived from the following sources: * Raw data collected during experimental work