DOI: 10.5061/dryad.msbcc2fz2
title: The 153 readable DNA sequences of a diet research on Eurasian otter of Kinmen Island

description: It is well known that Eurasian otters principally feed on fishes and
 crustaceans, however, their detailed diet taxonomies are not fully
 understood. I performed a series of Sanger-sequencing reactions, utilizing
 nine primer sets for Eurasian otter diet research. In these data
 materials, in total 153 readable DNA sequences of a diet research on
 Eurasian otter of Kinmen Island are presented. Such information provides a
 very detailed insight of feeding behavior of the Eurasian otter in Kinmen.
 These sequences were sequenced from 49 DNA samples extracted from fresh
 spraints collected from Kinmen from April 2017 to November 2018. Each DNA
 sample was PCR-amplified 9 times and with 9 primer sets. Eight of them are
 published universal COI primers and targeted toward different prey taxa.
 Besides, I designed a group of COIII primers for the introduced tilapia,
 the most abundant prey species in Kinmen Island. In total, 16 prey types
 were identified across all 49 samples. Furthermore, another identified
 sequence group comprised “non-food species”, including bacteria, amoeba,
 diatoms, small insects (e.g. ants and mosquitos) and small
 invertebrates, as well as the otters’ sequences themselves. The detailed
 information of these 153 sequences including collection sites, primers,
 identified food/non-food species, linkage of blast results, etc. are
 listed in S2.xlxs; for the sequences themselves are listed in S3.txt.
description: These data were sequenced from 49 DNA samples extracted from
 fresh spraints collected from Kinmen from April 2017 to November 2018.
 Each DNA sample was PCR-amplified 9 times by Sanger sequencing method and
 with 9 primer sets. Eight of them are published universal COI primers and
 targeted toward different prey taxa. They are (I) forward primer BirdF1
 and reverse primer cocktails BirdR1+R2+R (BirdRM) for avian species; (II)
 forward primer VF1 and reverse primer cocktails VR1+VR1d (VRM) for
 mammals, reptiles, fish, amphibians, and some insects; (III) forward
 primer chmf4 and reverse primer chmr4 for amphibians; (IV) forward primer
 FF2d and reverse primer FR1d for fishes; (V) forward primer FishF1 and
 reverse primer FishR1 for fishes; (VI) forward primer FishF2 and reverse
 primer FishR2 for fishes; (VII) forward primer LCO1490 and reverse primer
 HCO2198 for various phyla from the animal kingdom; (VIII) forward primer
 LepF1 and reverse primer LepR1 for Lepidoptera. Besides, I designed a
 group of COIII primers for the introduced tilapia, the most abundant prey
 species in Kinmen Island. They are (IX) forward primer Til9020F and
 reverse primer cocktails Mos9516R+Nil9464R+Esc9305R+Zil9212R (TilMR). The
 PCR thermal cycling conditions consisted of 5 min at 94 °C, 40 cycles of
 30 sec at 94 °C, 30 sec at 50 °C, 50 sec at 72 °C, and a final step of 5
 min at 72 °C.
description: S2.xlxs The detailed information
 of 153 readable DNA sequences including collection sites, primers,
 identified food/non-food species, linkage of blast results,
 etc.  S3.txt
 The 153 DNA sequences in this study.