DOI: 10.22002/54e6p-fh461
title: NR1769pKM101conjFM433

description: skinny ecoli conjugation with FM433 ecoli
description: <strong>Tilt Series Date:</strong> 2014-10-25</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> NR1769 pKM101</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christian Baron</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown on a gold grid in a drop of 1:1 mix of bacteria for 30min</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-14/rawdata/</p>NRpKMplusFM433_conj1_18K0009.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
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        NRpKMplusFM433_conj1_18K0009_full.rec,  Reconstruction (Pixel Size 0.0 nm), 980.0 MB  
        
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        slicer000.tif,  2.7 MB  
        
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        slicer001.tif,  2.7 MB  
        
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        slicer002.tif,  2.7 MB  
        
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        slicer003.tif,  2.7 MB  
        
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        slicer004.tif,  2.7 MB  
        
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        slicer005.tif,  2.7 MB  
        
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        slicer006.tif,  2.7 MB  
        
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        slicer007.tif,  2.7 MB  
        
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        slicer008.tif,  2.7 MB  
        
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        slicer009.tif,  2.7 MB  
        
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        slicer010.tif,  2.7 MB  
        
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        slicer011.tif,  2.7 MB  
        
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