DOI: 10.5061/dryad.6q573n64w
title: Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera

description: In this study, we detailed the indicators of development of H.
 armigera, such as growth and development period, number of
 surviving larvae, body weight, pupa weight, and number, as well
 as the relative expression levels of HaSumf1 gene at different instars,
 and the third- and fifth-instars after feeding with glucosinolates and /or
 dsSumf1.
description: (1) <em>H. armigera</em> Rearing
 (2) Bioinformatics Analysis (3) Total RNA
 Extraction, cDNA Synthesis, and <em>HaSumf1</em> Determination
 in Different Stages (4) Cloning and Sequencing
 <em>HaSumf1</em> (5)  Synthesis of dsRNA in
 Vitro (6) Effects of Silencing HaSumf1 on the Growth
 and Development of <em>H. armigera</em> (7)
 Gene Expression after Silencing <em>HaSumf1</em>
 (8) Statistical Analysis
description: GENERAL INFORMATION 1. Title of Dataset: Functions of the
 Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host
 glucosinolates adaptation of Helicoverpa armigera [Access this dataset on
 Dryad](https://doi.org/10.5061/dryad.6q573n64w) 2. Author Information A.
 Principal Investigator Contact Information Name: Long Zhang Institution:
 Xinjiang University Email: B. Associate or
 Co-investigator Contact Information Name: Zhiran Zhou Institution:
 Xinjiang University Name: Fan Cai Institution: Xinjiang University Name:
 Wanli Qin Institution: Xinjiang University Name: Li Liu Institution:
 Xinjiang University Name: Guli Kuerban Institution: Xinjiang University
 Name: Lin Jin Institution: Nanjing Agricultural University Name: Xiaoli Ma
 Institution: Xinjiang University 3. Information about funding sources:
 National Natural Science Foundation of China, Award: 32060620; Natural
 Science Foundation of the Autonomous Region, Award: 2020D01C060
 SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data:
 CC0 1.0 Universal (CC0 1.0) Public Domain 2. Links to publications that
 cite or use the data: Zhang, L., Zhou, Z., Cai, F., Qin, W., Liu, L.,
 Kuerban, G., Jin, L., Ma, X. (2023). Functions of the Sulfatase-Modifying
 Factor 1 (HaSumf1) in the development and host glucosinolates adaptation
 of Helicoverpa armigera. Journal of Applied Entomology. 3. Links to other
 publicly accessible locations of the data: None 4. Links/relationships to
 ancillary data sets: None 5. Was data derived from another source? No A.
 If yes, list source(s): NA 6. Recommended citation for this dataset:
 Zhang, L., Zhou, Z., Cai, F., Qin, W., Liu, L., Kuerban, G., Jin, L., Ma,
 X. (2023). Data from: Functions of the Sulfatase-Modifying Factor 1
 (HaSumf1) in the development and host glucosinolates adaptation of
 Helicoverpa armigera. Dryad Digital Repository.
 DATA & FILE OVERVIEW
 In this study, we detailed the indicators of development of H. armigera,
 such as growth and development period from first- to fifth-instar, number
 and weight of surviving larvae at fifth-instar and pupa, as well as the
 relative expression levels of HaSumf1 gene at different instars and the
 third- and fifth-instars after feeding with glucosinolates and/or dsSumf1.
 1. File List: A) Expression of HaSumf1 at different instars.csv B)
 Expression of HaSumf1 at third-instar.csv C) Expression of HaSumf1 at
 fifth-instar.csv D) Growth and development period of larvae.csv E) Number
 of surviving larvae.csv F) Number of surviving pupa.csv G) Weight of
 surviving larvae.csv H) Weight of surviving pupa.csv 2. Relationship
 between files, if important: None 3. Additional related data collected
 that was not included in the current data package: None 4. Are there
 multiple versions of the dataset? No A. If yes, name of file(s) that was
 updated: NA i. Why was the file updated? NA ii. When was the file updated?
 NA DATA-SPECIFIC INFORMATION FOR: Expression of HaSumf1 at different
 instars.csv 1. Number of variables: 9 2. Number of cases/rows: 27 3.
 Variable List: * Instars: First-instar, Second-instar, Third-instar,
 Fourth-instar, Fifth-instar, Prepupa, Pupa, Male adult, Female adult * CT
 (HaSumf1): Threshold cycle (CT) of HaSumf1 measured by qRT-PCR * CT
 (RPS15): Threshold cycle (CT) of RPS15 ( internal control genes) measured
 by qRT-PCR * CT_average (RPS15): Average value of threshold cycle (CT) of
 RPS15 ( internal control genes) measured by qRT-PCR *
 Delta_CT=CT(HaSumf1)-CT_average (RPS15): D-value between CT (HaSumf1) and
 CT (RPS15) * Delta_CT (average): Average value of D-value between CT
 (HaSumf1) and CT (RPS15) * 2 Delta_CT=Delta_CT(HaSumf1)-Delta_CT(RPS15):
 D-value between Delta_CT(HaSumf1) and Delta_CT(average) * 2^(-2 Delta_CT):
 Relative expression level of HaSumf1 gene * Average: Average value of
 relative expression level of HaSumf1 gene 4. Missing data codes:
 'n/a' 5. Specialized formats or other abbreviations used: None
 DATA-SPECIFIC INFORMATION FOR: Expression of HaSumf1 at third-instar.csv
 1. Number of variables: 11 2. Number of cases/rows: 27 3. Variable List: *
 Groups: Control (feeding normal diet and pure water), dsEGFP (feeding
 normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1),
 0.004%GLS (feeding diet containing 0.004%GLS and pure water),
 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP),
 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1),
 0.04%GLS (feeding diet containing 0.04%GLS and pure water),
 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP),
 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1) * CT
 (HaSumf1): Threshold cycle (CT) of HaSumf1 measured by qRT-PCR * CT
 (RPS15): Threshold cycle (CT) of RPS15 ( internal control genes) measured
 by qRT-PCR * CT_average (RPS15): Average value of threshold cycle (CT) of
 RPS15 ( internal control genes) measured by qRT-PCR *
 Delta_CT=CT(HaSumf1)-CT_average (RPS15): D-value between CT (HaSumf1) and
 CT (RPS15) * Delta_CT (average): Average value of D-value between CT
 (HaSumf1) and CT (RPS15) * 2 Delta_CT=Delta_CT(HaSumf1)-Delta_CT(RPS15):
 D-value between Delta_CT(HaSumf1) and Delta_CT(average) * 2^(-2 Delta_CT):
 Relative expression level of HaSumf1 gene * Average: Average value of
 relative expression level of HaSumf1 gene * Treatment-Control: Changes in
 the treatment group compared to the control group *
 (Treatment-Control)/Control: The ratio of changes in the treatment group
 compared to the control group 4. Missing data codes: 'n/a' 5.
 Specialized formats or other abbreviations used: None DATA-SPECIFIC
 INFORMATION FOR: Expression of HaSumf1 at fifth-instar.csv 1. Number of
 variables: 11 2. Number of cases/rows: 27 3. Variable List: * Groups:
 Control (feeding normal diet and pure water), dsEGFP (feeding normal diet
 and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding
 diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet
 containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet
 containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing
 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing
 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS
 and dsSumf1) * CT (HaSumf1): Threshold cycle (CT) of HaSumf1 measured by
 qRT-PCR * CT (RPS15): Threshold cycle (CT) of RPS15 (internal control
 genes) measured by qRT-PCR * CT_average (RPS15): Average value of
 threshold cycle (CT) of RPS15 (internal control genes) measured by qRT-PCR
 * Delta_CT=CT(HaSumf1)-CT_average (RPS15): D-value between CT (HaSumf1)
 and CT (RPS15) * Delta_CT (average): Average value of D-value between CT
 (HaSumf1) and CT (RPS15) * 2 Delta_CT=Delta_CT(HaSumf1)-Delta_CT(RPS15):
 D-value between Delta_CT(HaSumf1) and Delta_CT(average) * 2^(-2 Delta_CT):
 Relative expression level of HaSumf1 gene * Average: Average value of
 relative expression level of HaSumf1 gene * Treatment-Control: Changes in
 the treatment group compared to the control group *
 (Treatment-Control)/Control: The ratio of changes in the treatment group
 compared to the control group 4. Missing data codes: 'n/a' 5.
 Specialized formats or other abbreviations used: None DATA-SPECIFIC
 INFORMATION FOR: Growth and development period of larvae.csv 1. Number of
 variables: 8 2. Number of cases/rows: 495 3. Variable List: * Treatment:
 Control (feeding normal diet and pure water), dsEGFP (feeding normal diet
 and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding
 diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet
 containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet
 containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing
 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing
 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS
 and dsSumf1) * Groups: Each Treatment is divided into 3 groups A
 (including 18 samples), B (including 18 samples), and C (including 19
 samples) * Number: Number of each sample * First_instar: The period from
 hatching of the first larva to individual rearing of each larva is 2 days
 * Second_instar: The period from individual rearing of each larva to
 molting at second-instar, in days * Third_instar: The period from molting
 at second-instar to molting at third-instar, in days * Fourth_instar: The
 period from molting at third-instar to molting at fourth-instar, in days *
 Fifth_instar: The period from molting at fourth-instar to molting at
 fifth-instar, in days 4. Missing data codes: 'n/a' represents
 the death sample 5. Specialized formats or other abbreviations used: The
 developmental duration of larvae (the duration of the entire larval stage
 from first- to fifth-instar) DATA-SPECIFIC INFORMATION FOR: Weight of
 surviving larvae.csv 1. Number of variables: 4 2. Number of cases/rows:
 495 3. Variable List: * Treatment: Control (feeding normal diet and pure
 water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal
 diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure
 water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP),
 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1),
 0.04%GLS (feeding diet containing 0.04%GLS and pure water),
 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP),
 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1) * Groups:
 Each Treatment is divided into 3 groups A (including 18 samples), B
 (including 18 samples), and C (including 19 samples) * Number: Number of
 each sample * Body weight (mg): The larval body weight measurements within
 24-36 hours of molting at fifth-instar 4. Missing data codes:
 'n/a' represents the death sample 5. Specialized formats or
 other abbreviations used: None DATA-SPECIFIC INFORMATION FOR: Weight of
 surviving pupa.csv 1. Number of variables: 4 2. Number of cases/rows: 495
 3. Variable List: * Treatment: Control (feeding normal diet and pure
 water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal
 diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure
 water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP),
 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1),
 0.04%GLS (feeding diet containing 0.04%GLS and pure water),
 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP),
 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1) * Groups:
 Each Treatment is divided into 3 groups A (including 18 samples), B
 (including 18 samples), and C (including 19 samples) * Number: Number of
 each sample * Pupa weight (mg): The pupa weight measurements within 24-36
 hours of pupation 4. Missing data codes: 'n/a' represents the
 death sample 5. Specialized formats or other abbreviations used: None
 DATA-SPECIFIC INFORMATION FOR: Number of surviving larvae.csv 1. Number of
 variables: 4 2. Number of cases/rows: 27 3. Variable List: * Treatment:
 Control (feeding normal diet and pure water), dsEGFP (feeding normal diet
 and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding
 diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet
 containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet
 containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing
 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing
 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS
 and dsSumf1) * Groups: Each Treatment is divided into 3 groups A
 (including 18 samples), B (including 18 samples), and C (including 19
 samples) * Initial number of larvae: The number of larvae used for testing
 in groups A, B and C * Number of surviving larvae: Number of surviving
 larvae when measuring body weight at fifth-instar 4. Missing data codes:
 None 5. Specialized formats or other abbreviations used: The larval
 survival rate (the ratio of the number of surviving at fifth-instar to the
 initial number of larvae) DATA-SPECIFIC INFORMATION FOR: Number of
 surviving pupa.csv 1. Number of variables: 4 2. Number of cases/rows: 27
 3. Variable List: * Treatment: Control (feeding normal diet and pure
 water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal
 diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure
 water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP),
 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1),
 0.04%GLS (feeding diet containing 0.04%GLS and pure water),
 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP),
 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1) * Groups:
 Each Treatment is divided into 3 groups A (including 18 samples), B
 (including 18 samples), and C (including 19 samples) * Number of surviving
 larvae: Number of surviving larvae when measuring body weight at
 fifth-instar * Number of surviving pupa: Number of surviving pupa when
 measuring weight of pupa 4. Missing data codes: None 5. Specialized
 formats or other abbreviations used: The pupation rate (the ratio of the
 number of pupa to surviving larvae at fifth-instar)