cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/n8e34-cc447
title: Agrobacterium tumefaciens A139

description: Bald strain with GFP tagged VirB8
description: <strong>Tilt Series Date:</strong> 2014-01-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown on plate, scraped off and grown o/n in a drop</p>
<strong>Sample Preparation:</strong> Vitrobot manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-24/rawdata/</p>A139_drop_0021.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-24/rawdata/A139_drop_0021.mrc"
        > <i class="download icon"></i> Download </a></p>

        A139_drop_0021_full.rec,  Reconstruction (Pixel Size 0.0 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-01-27-24/3dimage_49300/A139_drop_0021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yynjw-ar164
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C126
description: <strong>Tilt Series Date:</strong> 2012-05-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-21/3dimage_69239/</p>12may04_C126_3_pnlDN_Cos7_AT4BT1_0003_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-21/3dimage_69239/12may04_C126_3_pnlDN_Cos7_AT4BT1_0003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may04_C126_3_pnlDN_Cos7_AT4BT1_0003.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-04-21/rawdata/12may04_C126_3_pnlDN_Cos7_AT4BT1_0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jn6vb-cka03
title: Agrobacterium tumefaciens

description: Bald strain, flagellin A, B and C missing. D is present
description: <strong>Tilt Series Date:</strong> 2013-10-30</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on AB medium and induced with AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
  etl2013-10-30-17_slicer20276.jpg: Flattening
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-17/rawdata/</p>bald20016.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-17/rawdata/bald20016.mrc"
        > <i class="download icon"></i> Download </a></p>

        bald20016_full.rec,  Reconstruction (Pixel Size 1.6 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-17/3dimage_33686/bald20016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sfs3c-xt069
title: M-MuLV 43D infected MEFs

description: M-MuLV 43D wildtype
description: <strong>Tilt Series Date:</strong> 2013-11-19</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> M-MuLV infected cells provided by Hung Fan (UC Irvine)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole MEFs infected with M-MuLV 43D were cultured on Au grids, blotted manually and plunge frozen.</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-19-17/rawdata/</p>2013nov19_C183_1_43D_at1bt1_0015.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-19-17/rawdata/2013nov19_C183_1_43D_at1bt1_0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        2013nov19_C183_1_43D_at1bt1_0015_full.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-19-17/3dimage_34559/2013nov19_C183_1_43D_at1bt1_0015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a5h87-ez942
title: HeLa

description: Synchronized, looking for midbodies
description: <strong>Tilt Series Date:</strong> 2014-01-06</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF tomo</p>
<strong>Collaborators and Roles:</strong> With Doug Mckay and Katherine Ullman</p>
<strong>Sample Preparation:</strong> HeLa cells+ siRNA NUP 153+ Thymidine 2mM. 14 hour after release</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-32/rawdata/</p>midbody10012.mrc,  Tilt Series (Pixel Size 0.6 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-32/rawdata/midbody10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        midbody10012_full.rec,  Reconstruction (Pixel Size 2.4 nm), 156.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-01-06-32/3dimage_50444/midbody10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/zzqxf-tt694
title: E. coli pED208

description: E. coli with plasmid pED208
description: <strong>Tilt Series Date:</strong> 2014-12-16</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells arrived dead.</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-10/rawdata/</p>pED2080002.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-10/rawdata/pED2080002.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED2080002_full.rec,  Reconstruction (Pixel Size 2.52 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-12-16-10/3dimage_48796/pED2080002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ara8w-snt29
title: HUVECs 24h PI pNLBglI

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 6500
description: <strong>Tilt Series Date:</strong> 2013-04-30</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 6500x. </p>
<strong>Microscope:</strong> UCLA Titan</p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> FEIbatchtomo</p>
<strong>Collaborators and Roles:</strong> Collected at UCLA with FEI Batchtomo</p>
<strong>Sample Preparation:</strong> HUVECs imaged 24 hours after infection with pNLBglI. The goal is to image maturing HIV at the edge of infected cells.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-9/rawdata/</p>13apr26_tomo_16.mrc,  Tilt Series (Pixel Size 1.35 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-9/rawdata/13apr26_tomo_16.mrc"
        > <i class="download icon"></i> Download </a></p>

        13apr26_tomo_16_full.rec,  Reconstruction (Pixel Size 2.7 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-9/3dimage_26327/13apr26_tomo_16_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9z65b-8xv10
title: Bsubtilis_wildtype_168_sacculi

description: Bsubtilis_168_sacculi_withTA
description: <strong>Tilt Series Date:</strong> 2012-09-01</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> subsp. subtilis str. 168</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-01-11/rawdata/</p>mj_thin0006.mrc,  Tilt Series (Pixel Size 0.5 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-01-11/rawdata/mj_thin0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        mj_thin5_full.rec,  Reconstruction (Pixel Size 2.0 nm), 742.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-09-01-11/3dimage_25655/mj_thin5_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/svan6-3gs39
title: Agrobacterium tumefaciens

description: C58 wild type
description: <strong>Tilt Series Date:</strong> 2013-07-28</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Polara</p>
<strong>Collaborators and Roles:</strong> Pat Zabryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> C58 wild type</p>
<strong>Sample Preparation:</strong> 200uM AS, grown on agar + liquid for 2 days</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-28-18/rawdata/</p>c58_au20018.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-28-18/rawdata/c58_au20018.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_au20018_part121_64.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-07-28-18/3dimage_29433/c58_au20018_part121_64.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wnb6e-jf248
title: JPA 1558 LMEM

description: JPA 1558 TlpT - YFPlabelled cytoplasmic receptor correlative cryo-LMECT experiment (LM from 051512)
description: <strong>Tilt Series Date:</strong> 2012-05-16</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-44.0°, 56.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -12.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Sux media at 30 degrees, Ampicillin treated for one hour (2.57ml sux plus 0.0113g Ampicillin)membrane dye FM64X added 10 min at room temp before antibiotic treatment for one hour</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-1/rawdata/</p>1573_LMEM1.mrc,  Tilt Series (Pixel Size 0.78 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-1/rawdata/1573_LMEM1.mrc"
        > <i class="download icon"></i> Download </a></p>

        1573_LMEM1_full.rec,  Reconstruction (Pixel Size 1.56 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-1/3dimage_18633/1573_LMEM1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q2je2-9hx65
title: Crosslinked HIV-1 cores TRIM5a

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5alpha.
description: <strong>Tilt Series Date:</strong> 2013-05-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -6.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor and SIRT reconstruction</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-13/3dimage_42968/</p>atlas0013_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-13/3dimage_42968/atlas0013_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas0013.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2013-05-25-13/rawdata/atlas0013.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b3wvq-xsh50
title: Streptococcus pneumoniae

description: over expression of SP_0107 PG hydroalse
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Streptococcus pneumoniae</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Andrew Camili</p>
<strong>Purification / Growth Conditions / Treatment:</strong> over expression of SP_0107 PG hydroalse</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-13/rawdata/</p>overexpr0013.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-13/rawdata/overexpr0013.mrc"
        > <i class="download icon"></i> Download </a></p>

        overexpr0013_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-13/3dimage_30557/overexpr0013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m8t6a-epd15
title: Agrobacterium tumefaciens

description: C58 grown on slide
description: <strong>Tilt Series Date:</strong> 2013-10-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on a glass slide</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-4/rawdata/</p>c58_slide_2750012.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-4/rawdata/c58_slide_2750012.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_slide_2750012_full.rec,  Reconstruction (Pixel Size 1.56 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-4/3dimage_31926/c58_slide_2750012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bx6q9-6nb12
title: HUVECs infx pNLGFP

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 19000.
description: <strong>Tilt Series Date:</strong> 2013-07-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Sample Preparation:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-06-19/rawdata/</p>13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0033.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
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        13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0033_part121_58.rec,  Reconstruction (Pixel Size 2.24 nm), 602.8 MB  
        
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