cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/mxa0t-md780
title: nanowire+doublepili mutant+Chemo

description: This sample is batch 2 collection. This is from double pili mutant run on a chemostat.Batch 1 collection was from WT grown in serum bottle
description: <strong>Tilt Series Date:</strong> 2015-04-11</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh, USC</p>
<strong>Sample Preparation:</strong> Went to USC and froze using manual vitrobot. Only 1 grid was good among 6 and cells were isolated so couldn't detect any nanowires so far</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-11-6/rawdata/</p>4_atlas_nanowires0001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-11-6/rawdata/4_atlas_nanowires0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        4_atlas_nanowires0001_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-11-6/3dimage_53847/4_atlas_nanowires0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v4md9-msx88
title: T5_1693Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1693 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-04-02</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot.</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-04-02-29/rawdata/</p>cys1693_sq48_4sqs0006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-04-02-29/rawdata/cys1693_sq48_4sqs0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        cys1693_sq48_4sqs0006.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-04-02-29/3dimage_83451/cys1693_sq48_4sqs0006.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eb5je-qvj60
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-238/rawdata/</p>T4SSWTDec12A083_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-238/rawdata/T4SSWTDec12A083_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTDec12A083_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-238/3dimage_105004/T4SSWTDec12A083_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1xqhq-r7564
title: Vibrio cholerae delta TcpB

description: Raw data files of Vibrio cholerae delta TcpB
description: <strong>Tilt Series Date:</strong> 2015-10-16</p>
<strong>Data Taken By:</strong> Andreas Kjaer</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown ON in LB with ampicillin and IPTG, refreshed in LB shortly prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-110/3dimage_73344/</p>10162015_TcpB_10110_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-110/3dimage_73344/10162015_TcpB_10110_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        10162015_TcpB_10110.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-110/rawdata/10162015_TcpB_10110.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ha6sx-pct04
title: CLEM of TRIM5a-YFP in HeLa cells

description: HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads 1/300. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS
description: <strong>Tilt Series Date:</strong> 2015-01-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 234.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-5/rawdata/</p>tomgraphytest13.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-5/rawdata/tomgraphytest13.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomgraphytest13_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-22-5/3dimage_51215/tomgraphytest13_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gfrjy-05z08
title: nanochemowt_2ndday

description: This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.
description: <strong>Tilt Series Date:</strong> 2015-06-06</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Sample Preparation:</strong> This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-42/rawdata/</p>nanochemowt2day_atlas30002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-42/rawdata/nanochemowt2day_atlas30002.mrc"
        > <i class="download icon"></i> Download </a></p>

        nanochemowt2day_atlas30002_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-06-06-42/3dimage_58154/nanochemowt2day_atlas30002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/svs66-sdd69
title: NR1769/R1-16 conjugation

description: Grid box YWC241-2
description: <strong>Tilt Series Date:</strong> 2017-02-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
  ycw2017-02-07-12_slicer17780.jpg: T4SS with basal body and MS2 phage on pilus ycw2017-02-07-12_slicer17781.jpg: MS2 phage labeling F pilus ycw2017-02-07-12_slicer17787.jpg: non-piliated T4SS? ycw2017-02-07-12_slicer18068.jpg: F-pilus basal body
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-07-12/rawdata/</p>20170207_YWC241_10012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-07-12/rawdata/20170207_YWC241_10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20170207_YWC241_10012_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-07-12/3dimage_82142/20170207_YWC241_10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5519/6j4aecuk
title: Micropalaeontology Collection citations and visitor numbers

description: A dataset to support the figures in a publication in Revue de Micropaleontologie on the Natural History Museum Microfossil Collection. Data derived mainly from annotations in museum specimen registers and museum visitor books to the Foraminifera and fossil Ostracoda collections . These have been added to with more recent citations for collections yet to be fully digitised.
DOI: 10.22002/4g4hw-bm054
title: JV9264

description: Raw data files of JV9264
description: <strong>Tilt Series Date:</strong> 2018-09-06</p>
<strong>Data Taken By:</strong> Jun Zhang</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degree and OD 2.5-3.0</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-78/rawdata/</p>JV9264_B17.mrc,  Tilt Series (Pixel Size 0.2654 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-78/rawdata/JV9264_B17.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9264_B17_part53_1.rec,  Reconstruction (Pixel Size 1.0616 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-06-78/3dimage_97927/JV9264_B17_part53_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7e7ry-r9p11
title: Dual-labeled VSVG-HIV

description: Raw data files of Dual-labeled VSVG-HIV
description: <strong>Tilt Series Date:</strong> 2019-02-01</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> iMOD</p>
<strong>Collaborators and Roles:</strong> Thomas J. Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degrees celsius</p>
<strong>Sample Preparation:</strong> plunge-freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-01-27/rawdata/</p>V27.mrc,  Tilt Series (Pixel Size 0.351 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-01-27/rawdata/V27.mrc"
        > <i class="download icon"></i> Download </a></p>

        V27_full.rec,  Reconstruction (Pixel Size 1.404 nm), 267.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-02-01-27/3dimage_101356/V27_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/e43b5-14z03
title: JV9264

description: Raw data files of JV9264
description: <strong>Tilt Series Date:</strong> 2018-09-10</p>
<strong>Data Taken By:</strong> Jun Zhang</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -14.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joseph</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 37 degree and OD 2.5-3.0</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-10-50/rawdata/</p>JV9264_TS55.mrc,  Tilt Series (Pixel Size 0.2654 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-10-50/rawdata/JV9264_TS55.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9264_TS55_part55_3.rec,  Reconstruction (Pixel Size 1.0616 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jza2018-09-10-50/3dimage_98186/JV9264_TS55_part55_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x7np0-npb74
title: Legionella cells for the flagell

description: Raw data files of Legionella cells for the flagell
description: <strong>Tilt Series Date:</strong> 2018-03-13</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were grown in LB overnight.</p>
<strong>Sample Preparation:</strong> Grids were prepared from an overnight LB culture.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-11/rawdata/</p>cell19.mrc,  Tilt Series (Pixel Size 0.39 nm), 682.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-11/rawdata/cell19.mrc"
        > <i class="download icon"></i> Download </a></p>

        cell19_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-11/3dimage_92187/cell19_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x9vsb-0cb61
title: Legionella cells for the flagell

description: Raw data files of Legionella cells for the flagell
description: <strong>Tilt Series Date:</strong> 2018-03-13</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were grown in LB overnight.</p>
<strong>Sample Preparation:</strong> Grids were prepared from an overnight LB culture.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-66/rawdata/</p>Legionella_flagella37.mrc,  Tilt Series (Pixel Size 0.39 nm), 767.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-66/rawdata/Legionella_flagella37.mrc"
        > <i class="download icon"></i> Download </a></p>

        Legionella_flagella37_full.rec,  Reconstruction (Pixel Size 1.56 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-03-13-66/3dimage_92284/Legionella_flagella37_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16995421
title: Diversity and environmental data sets for Multitrophic alpha and beta diversity of lake plankton across Canada

description: The file “MTI_ENVdata.xlsx” contains environmental variables from 559 sampled as part of the NSERC Canadian Lake Pulse Network project over three summers (2017-2018-2019). Lake morphometry variables were either measured on site (lake depth), or obtained from HydroLAKES v. 1.0 (Messager et al. 2016). Water quality variables (water physical and chemical properties) were collected or measured at the deepest point of each lake, following the protocols from the NSERC Canadian Lake Pulse Network (2021). Watershed land use fractions were characterized for each lake, as described by Huot et al. (2019). Variables sampling depth and units are described in “metadata_variables.xlsx”. Annual mean air temperature was obtained using WorldClim version 2.1 climate data for the years 1970-2000. 

 

The file "MTI_DIVERSITYdata.csv" contains bacterio-, phyto- and zooplankton richness, shannond diversity and local contribution to diversity (LCBD) indices calculated in 559 lakes across canada. The 'simple' approach used specnumber from the library vegan to compute richness, the function diversity in vegan for Shannon diversity and the function beta.div from the library adespatial for LCBD.  In the 'threshold' method, diversity was calculated separately for each taxonomic order of bacterio-, phyto-, or zooplankton, and then compared with the maximum richness observed for that order across all lakes. Then, for any given lake, the number of orders that reached 30 % of maximum diversity were tallied, with X being an empirically derived threshold. Simple and threshold multitrophic richness and LCBD was calculated by scaling and then averaging values for each group.

References

Huot, Y., C. A. Brown, G. Potvin, and others. 2019. The NSERC Canadian Lake Pulse Network: A national assessment of lake health providing science for water management in a changing climate. Sci. Total Environ. 695: 133668. doi:10.1016/j.scitotenv.2019.133668

Messager, M. L., B. Lehner, G. Grill, I. Nedeva, and O. Schmitt. 2016. Estimating the volume and age of water stored in global lakes using a geo-statistical approach. Nat. Commun. 7: 1–11. doi:10.1038/ncomms13603

NSERC Canadian Lake Pulse Network. 2021. NSERC Canadian Lake Pulse Network field manual 2017 - 2018 - 2019 surveys, M.-P. Varin, M.-L. Beaulieu, and Y. Huot [eds.]. Université de Sherbrooke.