DOI: 10.5061/dryad.j6q573nmf
title: Data from: Root-exuded benzoxazinoids can alleviate negative plant-soil feedbacks

description: Plants can suppress the growth of other plants by modifying soil
 properties. These negative plant-soil feedbacks are often
 species-specific, suggesting that some plants possess resistance
 strategies. However, the underlying mechanisms remain largely unknown.
 Here, we investigated if and how benzoxazinoids, a class of dominant
 secondary metabolites that are exuded into the soil by maize and other
 cereals, influence plant-soil feedbacks. We find that three out of five
 tested crop species suppress maize (Zea mays) performance via negative
 plant-soil feedbacks relative to the mean across species. This effect is
 partially alleviated by the capacity of maize plants to produce
 benzoxazinoids. Soil complementation with purified benzoxazinoids is
 sufficient to restore the protective effect for benzoxazinoid-deficient
 mutants. Sterilization and re-inoculation experiments suggest that
 benzoxazinoid-mediated protection acts via changes in soil biota.
 Substantial variation of the protective effect between experiments and
 soil types illustrates that its magnitude is context-dependent. In
 summary, our study demonstrates that plant secondary metabolites can
 confer resistance to negative plant-soil feedbacks. These findings expand
 the functional repertoire of plant secondary metabolites and reveal a
 mechanism by which plants can resist negative soil feedbacks. The
 uncovered phenomenon may represent a promising avenue to stabilize plant
 performance in crop rotations in the future.
description: # Data from: Root-exuded benzoxazinoids can alleviate negative plant-soil
 feedbacks This repository
 contains the data for the article published in New Phytologist:
 Root-exuded benzoxazinoids can alleviate negative plant-soil feedbacks
 Valentin Gfeller, Lisa Thoenen, Matthias Erb ## Description of the data
 and file structure All data sheets are named according to the figures in
 the manuscript. Description of the column names: Fig.1 *
 "pre_crop" and "Pre_crop_long" = the name of the
 precrop * "trt" = maize genotype ("w" = wild-type
 maize, "b" = bx1 mutant maize) * "height_harvest" =
 shoot height in cm * "dw_harvest" = shoot dry weight in g *
 "compound" = name of compound measured * "Area" = area
 under the curve for a given compound * "conc" = concentration of
 a given compound in ng/mL of soil Fig.2 * "maize_line" = the
 name of the maize line (B73 or W22) * "soil" = origin of the
 soil (Field Q-Matte or field in Changins) * "trt" = maize
 genotype ("w" = wild-type maize, "b" = bx1 mutant
 maize) * "precrop" = the name of the precrop *
 "height_3weeks" = shoot height 3 weeks after sowing in cm *
 "height_6weeks" = shoot height at harvest in cm *
 "dw_6weeks" = shoot dry weight at harvest in g Fig.3 *
 "trt" = maize genotype ("w" = wild-type maize,
 "b" = bx1 mutant maize) * "height_d..XX.." = shoot
 height ..X.. days after sowing in cm * "dry_weight_d27" = shoot
 dry weight at harvest in g * "chloro_d27" = Chlorophyll content
 at harvest (SPAD value) Fig.4 * "precrop" = the name of the
 precrop * "trt" = maize genotype ("w" = wild-type
 maize, "b" = bx1 mutant maize) * "x_ray" = soil
 sterilization with X-ray * "inoculate" = inoculation with living
 soil ("no" = no inoculation, "Tri" = inoculation with
 Triticum aestivum conditioned soil) * "complementation" =
 complementation treatment ("no" = control without
 benzoxazinoids, "yes" = complementation with benzoxazionids) *
 "height_harvest" = shoot height at harvest in cm *
 "dw_harvest" = shoot dry weight at harvest in g *
 "chloro_harvest" = Chlorophyll content at harvest (SPAD value)
 Fig.5 * "precrop" = the name of the precrop * "trt" =
 maize genotype ("w" = wild-type maize, "b" = bx1
 mutant maize) * "sterilization" = soil sterilization with X-ray
 * "inoculate" = inoculation with living soil ("no" =
 no inoculation, "Tri" = inoculation with Triticum aestivum
 conditioned soil, "Med" = inoculation with Medicago sativa
 conditioned soil) * "height_harvest" = shoot height at harvest
 in cm * "dw_harvest" = shoot dry weight at harvest in g *
 "chloro_harvest" = Chlorophyll content at harvest (SPAD value)
 The data sheet for Fig.5(a, b, c) is stored under Fig.4
 ("Phenotypes_maize_rep1.csv") Fig.6 The data sheet for Fig.6 is
 stored under Fig.5 ("Phenotypes_maize_rep3b.csv") Fig.S2 *
 "pre_crop" and "Pre_crop_long" = the name of the
 precrop * "dw_precrop" = shoot dry weight in g The data sheet
 for Fig.S2(b) is stored under Fig.1 ("Phenotypes_maize_1.csv")
 Fig.S3 * "soil" = origin of the soil (Field Q-Matte or field in
 Changins) * "precrop" = the name of the precrop *
 "dw_precrop" = shoot dry weight in g The data sheet for
 Fig.S3(b) is stored under Fig.2 ("Phen_maize_lin_soil.csv")
 Fig.S4 * "precrop" = the name of the precrop *
 "dw_precrop" = shoot dry weight in g Fig.S5 *
 "pre_crop" = the name of the precrop * "trt" = maize
 genotype ("w" = wild-type maize, "b" = bx1 mutant
 maize) * "compound" = name of compound measured *
 "Area" = area under the curve for a given compound *
 "ng_mL" = concentration of a given compound in ng/mL of soil *
 "BX_comp" = complementation treatment ("no" = control
 without benzoxazinoids, "comp" = complementation with
 benzoxazionids) * "complementation" = complementation treatment
 ("no" = control without benzoxazinoids, "BX" =
 complementation with benzoxazionids) * "complementation" =
 complementation treatment ("no" = control without
 benzoxazinoids, "comp" = complementation with benzoxazionids)