DOI: 10.5061/dryad.dj3rs70
title: Data from: Correlated evolution of self and interspecific incompatibility across the range of a Texas wildflower

description: Selection to prevent interspecific mating can cause an increase or a
 decrease in self pollination in sympatric populations. Characterizing
 geographic variation in self and interspecific incompatibilities within a
 species can reveal if and how the evolution of self and interspecific mate
 choice are linked. • We used controlled pollinations to characterize
 variation in self and interspecific incompatibility across 29 populations
 of Phlox drummondii. We evaluated seed set from these pollinations and
 described the developmental timing of variation in pollen-pistil
 compatibility. • There is extensive quantitative variation in
 self-incompatibility and interspecific-incompatibility with close congener
 P. cuspidata. P. drummondii populations that co-occur and hybridize with
 P. cuspidata have significantly higher interspecific incompatibility and
 self-incompatibility than geographically isolated P. drummondii
 populations. The strength of self and interspecific incompatibility is
 significantly correlated among individuals and the strength of both
 incompatibilities is explained by success of pollen adhesion to the
 stigma. • The correlated strength of self and interspecific
 incompatibility across the range of P. drummondii and the concurrent
 developmental timing of the pollen-pistil interaction, suggests these
 incompatibilities have an overlapping molecular mechanism. The geographic
 distribution of variation in incompatibilities indicates this mechanistic
 link between incompatibilities may affect the evolution of mate choice in
 plants.
description: Seed
 setSeed set. For each
 maternal plant grown in the greenhouse in 2014 and 2015 we show the number
 of flowers crossed and the number of seeds obtained for four types of
 pollinations - interspecific pollinations, intraspecific pollinations,
 self pollinations, and autogamy. We provide the geographic coordinates
 where the maternal seeds were collected.Tables_1_Dryad_Roda_&_Hopkins.txtClimatic dataClimatic data. Environmental data from P. drummondii populations as downloaded from the WorldClim v1.4 database in Sep 2016. We also show the loadings of the three first components in a PCA of this climatic data. Codes for climatic variables are: tmean = average monthly mean temperature (°C * 10); tmin = average monthly minimum temperature (°C * 10); tmax = average monthly maximum temperature (°C * 10); prec = average monthly precipitation (mm); alt = altitude (elevation above sea level) (m) (from SRTM); m = month of the year, from 1 (January) to 12 (December); x = code for the bioclimatic variable; bio1 = Annual Mean Temperature; bio2 = Mean Diurnal Range (Mean of monthly (max temp - min temp)); bio3 = Isothermality (bio2/bio7) (* 100); bio4 = Temperature Seasonality (standard deviation *100); bio5 = Max Temperature of Warmest Month; bio6 = Min Temperature of Coldest Month; bio7 = Temperature Annual Range (bio5-bio6); bio8 = Mean Temperature of Wettest Quarter; bio9 = Mean Temperature of Driest Quarter; bio10 = Mean Temperature of Warmest Quarter; bio11 = Mean Temperature of Coldest Quarter; bio12 = Annual Precipitation; bio13 = Precipitation of Wettest Month; bio14 = Precipitation of Driest Month; bio15 = Precipitation Seasonality (Coefficient of Variation); bio16 = Precipitation of Wettest Quarter; bio17 = Precipitation of Driest Quarter; bio18 = Precipitation of Warmest Quarter; bio19 = Precipitation of Coldest Quarter.Table_2_Dryad_Roda_&_Hopkins.txtPollen development observationsPollen development observations. We show results from observations of aniline blue stained pistils after three types of crosses, interspecific (Inter), Intraspecific (Intra), and self-cross (Self). The self compatibility phenotype of plants, as determined from seed counts (see methods), is provided: SC = self compatible; SI = self incompatible. Pistils were processed in batches, this information is included in the “Batchr” column.Table_3_Dryad_Roda_&_Hopkins.txtEarly pollen adhesionEarly pollen adhesion. We show results from observations of aniline blue stained pistils collected between 1 and 2 hours after pollination. We performed three types of crosses - interspecific pollinations (Inter), intraspecific pollinations (Intra), and self pollinations (Self).Table_4_Dryad_Roda_&_Hopkins.txt