cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8324705.v1
title: Species or population? Systematic status of Vieja coatlicue (Teleostei: Cichlidae)

description: ABSTRACT Pacific and Atlantic-slope rivers of the Isthmus of Tehuantepec in Mexico contain two cichlid species of the genus Vieja relevant to the current study, V. zonata and V. coatlicue, respectively. The Atlantic-slope species was initially considered a population of uncertain taxonomic status and recently described as a distinct species based on three putatively diagnostic morphological characters. The objective of this study was to combine new and existing genetic data, along with reanalysis of morphological characters, to test the distinctiveness of V. coatlicue. Little genetic variability and no geographic structuring were recovered. Additionally, putatively diagnostic morphological character states were present across both species, failing to separate the forms. The synthesis of molecular and morphological data supports the recognition of V. coatlicue as a junior synonym of V. zonata.
DOI: 10.6084/m9.figshare.8324693
title: Metaphylactic effect of calcium on milk composition and animal health in post-partum dairy cows

description: Abstract: The aim of this study was to evaluate the effects of subcutaneous calcium administration in post-partum dairy cows with respect to carbohydrate, protein and lipids metabolism, as well as its effects on milk composition. Twenty post-partum dairy cows were randomly divided into two groups (n=10): a control and a treated group with a single dose (150 mL) of calcium. Blood collection was performed on post-partum days 1, 2, 3, 7 and 10. In addition, we measured serum levels of total calcium, total proteins, albumin, globulins, glucose, cholesterol, triglycerides, as well as creatine kinase (CK) activity. We determined milk composition on post-partum days 5, 10 and 20. Serum total calcium levels was higher on post-partum days 3 and 7 in the treated group. Serum urea (day 3) and triglycerides (days 7 and 10) levels were higher in the treated group, as were CK activity levels at post-partum days 2 and 3. Serum glucose and cholesterol levels were lower on post-partum day 2 in treated animals. Fat (day 20) and lactose (day 10) content in milk samples were higher than in the treated animals. We concluded that treatment with calcium in cows during the post-partum period changes biochemical variables related to metabolism.
DOI: 10.6084/m9.figshare.8324693.v1
title: Metaphylactic effect of calcium on milk composition and animal health in post-partum dairy cows

description: Abstract: The aim of this study was to evaluate the effects of subcutaneous calcium administration in post-partum dairy cows with respect to carbohydrate, protein and lipids metabolism, as well as its effects on milk composition. Twenty post-partum dairy cows were randomly divided into two groups (n=10): a control and a treated group with a single dose (150 mL) of calcium. Blood collection was performed on post-partum days 1, 2, 3, 7 and 10. In addition, we measured serum levels of total calcium, total proteins, albumin, globulins, glucose, cholesterol, triglycerides, as well as creatine kinase (CK) activity. We determined milk composition on post-partum days 5, 10 and 20. Serum total calcium levels was higher on post-partum days 3 and 7 in the treated group. Serum urea (day 3) and triglycerides (days 7 and 10) levels were higher in the treated group, as were CK activity levels at post-partum days 2 and 3. Serum glucose and cholesterol levels were lower on post-partum day 2 in treated animals. Fat (day 20) and lactose (day 10) content in milk samples were higher than in the treated animals. We concluded that treatment with calcium in cows during the post-partum period changes biochemical variables related to metabolism.
DOI: 10.6084/m9.figshare.8324699
title: Three-Dimensional Reciprocal Invisibility Cloak with Multilayered Structure

description: Abstract We investigate a three-dimensional (3D) reciprocal invisibility cloak with a multilayered structure based on coordinate transformation theory and complementary media. There has been considerable research on invisibility cloaks such as the external cloak, which requires complex “anti-objects”, and closed cloaks of different shapes, in which the object enclosed is blind. Because all these are hard to realize in actual application, we apply a uniform layer to the whole construct into several parts in efforts to replace the ideal cloak with the aim to reduce complexity in actual fabrication. This multilayered cloak allows exchange of information without requiring “anti-objects”. All results were simulated and calculated in the Cartesian coordinate system and with the Comsol Multiphysics software. Electrical field simulations verified the efficiency of the designed cloak and the computed radar cross section (RCS) to illustrate the validity of this method and its efficiency in replacing the ideal cloak.
DOI: 10.6084/m9.figshare.8324699.v1
title: Three-Dimensional Reciprocal Invisibility Cloak with Multilayered Structure

description: Abstract We investigate a three-dimensional (3D) reciprocal invisibility cloak with a multilayered structure based on coordinate transformation theory and complementary media. There has been considerable research on invisibility cloaks such as the external cloak, which requires complex “anti-objects”, and closed cloaks of different shapes, in which the object enclosed is blind. Because all these are hard to realize in actual application, we apply a uniform layer to the whole construct into several parts in efforts to replace the ideal cloak with the aim to reduce complexity in actual fabrication. This multilayered cloak allows exchange of information without requiring “anti-objects”. All results were simulated and calculated in the Cartesian coordinate system and with the Comsol Multiphysics software. Electrical field simulations verified the efficiency of the designed cloak and the computed radar cross section (RCS) to illustrate the validity of this method and its efficiency in replacing the ideal cloak.
DOI: 10.6084/m9.figshare.8324690
title: Position-dependent correlation between TBX22 exon 5 methylation and palatal shelf fusion in the development of cleft palate

description: Abstract: DNA methylation is essential for spatiotemporally-regulated gene expression in embryonic development. TBX22 (Chr X: 107667964-107688978) functioning as a transcriptional repressor affects DNA binding, sumoylation, and transcriptional repression associated with X-linked cleft palate. This study aimed to explore the relationship and potential mechanism between TBX22 exon 5 methylation and palatal shelf fusion induced by all-trans retinoic acid (ATRA). We performed DNA methylation profiling, using MethylRAD-seq, after high throughput sequencing of mouse embryos from control (n=9) and ATRA-treated (to induce cleft palate, n=9) C57BL/6J mice at embryonic gestation days(E) 13.5, 14.5 and 16.5. TBX22 exon 5 was hyper-methylated at the CpG site at E13.5 (P=0.025, log2FC=1.5) and E14.5 (P=0.011, log2FC:1.5) in ATRA-treated, whereas methylation TBX22 exon 5 at the CpG site was not significantly different at E16.5 (P=0.808, log2FC=-0.2) between control and ATRA-treated. MSP results showed a similar trend consistent with the MethylRAD-seq results. qPCR showed the change in TBX22 exon 5 expression level negatively correlated with its TBX22 exon 5 methylation level. These results indicate that changes in TBX22 exon 5 methylation might play an important regulatory role during palatal shelf fusion, and may enlighten the development of novel epigenetic biomarkers in the treatment of CP in the future.
DOI: 10.6084/m9.figshare.8324687
title: Protective Effect of Vitamin C on Triptolide-induced Acute Hepatotoxicity in Mice through mitigation of oxidative stress

description: Abstract: Triptolide, a purified diterpenoid from the herb Tripterygium wilfordii Hook.f., was widely used to treat many diseases. However, the hepatotoxicity of triptolide limited its clinical use. Research showed oxidative stress played an important role in triptolide-induced liver injury. To investigate the effect of vitamin C, which was one of the most effective antioxidants, on triptolide-induced hepatotoxicity and its potential mechanism in mice. In the present study, acute liver injury was induced by intraperitoneal injection of triptolide and vitamin C was orally administered. The results showed treatment with vitamin C prevented the triptolide-induced liver injury by reducing the levels of aspartate transaminase from 286.86 to 192.48 U/mL and alanine aminotransferase from 746.75 to 203.36 U/mL. Histopathological changes of liver corresponded to the same trend. Furthermore, vitamin C also protected the liver against triptolide-induced oxidative stress by inhibiting the generation of malondialdehyde (2.22 to 1.49 nmol/mgprot) and hydrogen peroxide (14.74 to 7.19 mmol/gprot) and restoring the level of total superoxide dismutase (24.32 to 42.55 U/mgprot) and glutathione (7.69 to 13.03 μg/mgprot). These results indicated that vitamin C could protect against triptolide-induced liver injury via reducing oxidative stress, and vitamin C may pose a significant health protection in the clinical use of triptolide.
DOI: 10.6084/m9.figshare.8324690.v1
title: Position-dependent correlation between TBX22 exon 5 methylation and palatal shelf fusion in the development of cleft palate

description: Abstract: DNA methylation is essential for spatiotemporally-regulated gene expression in embryonic development. TBX22 (Chr X: 107667964-107688978) functioning as a transcriptional repressor affects DNA binding, sumoylation, and transcriptional repression associated with X-linked cleft palate. This study aimed to explore the relationship and potential mechanism between TBX22 exon 5 methylation and palatal shelf fusion induced by all-trans retinoic acid (ATRA). We performed DNA methylation profiling, using MethylRAD-seq, after high throughput sequencing of mouse embryos from control (n=9) and ATRA-treated (to induce cleft palate, n=9) C57BL/6J mice at embryonic gestation days(E) 13.5, 14.5 and 16.5. TBX22 exon 5 was hyper-methylated at the CpG site at E13.5 (P=0.025, log2FC=1.5) and E14.5 (P=0.011, log2FC:1.5) in ATRA-treated, whereas methylation TBX22 exon 5 at the CpG site was not significantly different at E16.5 (P=0.808, log2FC=-0.2) between control and ATRA-treated. MSP results showed a similar trend consistent with the MethylRAD-seq results. qPCR showed the change in TBX22 exon 5 expression level negatively correlated with its TBX22 exon 5 methylation level. These results indicate that changes in TBX22 exon 5 methylation might play an important regulatory role during palatal shelf fusion, and may enlighten the development of novel epigenetic biomarkers in the treatment of CP in the future.
DOI: 10.6084/m9.figshare.8324681
title: Combined index of genomic prediction methods applied to productivity

description: ABSTRACT: Rice cultivation has great national and global importance, being one of the most produced and consumed cereals in the world and the primary food for more than half of the world’s population. Because of its importance as food, developing efficient methods to select and predict genetically superior individuals in reference to plant traits is of extreme importance for breeding programs. The objective of this research was to evaluate and compare the efficiency of the Delta-p, G-BLUP (Genomic Best Linear Unbiased Predictor), BayesCpi, BLASSO (Bayesian Least Absolute Shrinkage and Selection Operator), Delta-p/G-BLUP index, Delta-p/BayesCpi index, and Delta-p/BLASSO index in the estimation of genomic values and the effects of single nucleotide polymorphisms on phenotypic data associated with rice traits. Use of molecular markers allowed high selective efficiency and increased genetic gain per unit time. The Delta-p method uses the concept of change in allelic frequency caused by selection and the theoretical concept of genetic gain. The Index is based on the principle of combined selection, using the information regarding the additive genomic values predicted via G-BLUP, BayesCpi, BLASSO, or Delta-p. These methods were applied and compared for genomic prediction using nine rice traits: flag leaf length, flag leaf width, panicles number per plant, primary panicle branch number, seed length, seed width, amylose content, protein content, and blast resistance. Delta-p/G-BLUP index had higher predictive abilities for the traits studied, except for amylose content trait in which the method with the highest predictive ability was BayesCpi, being approximately 3% greater than that of the Delta-p/G-BLUP index.
DOI: 10.6084/m9.figshare.8324687.v1
title: Protective Effect of Vitamin C on Triptolide-induced Acute Hepatotoxicity in Mice through mitigation of oxidative stress

description: Abstract: Triptolide, a purified diterpenoid from the herb Tripterygium wilfordii Hook.f., was widely used to treat many diseases. However, the hepatotoxicity of triptolide limited its clinical use. Research showed oxidative stress played an important role in triptolide-induced liver injury. To investigate the effect of vitamin C, which was one of the most effective antioxidants, on triptolide-induced hepatotoxicity and its potential mechanism in mice. In the present study, acute liver injury was induced by intraperitoneal injection of triptolide and vitamin C was orally administered. The results showed treatment with vitamin C prevented the triptolide-induced liver injury by reducing the levels of aspartate transaminase from 286.86 to 192.48 U/mL and alanine aminotransferase from 746.75 to 203.36 U/mL. Histopathological changes of liver corresponded to the same trend. Furthermore, vitamin C also protected the liver against triptolide-induced oxidative stress by inhibiting the generation of malondialdehyde (2.22 to 1.49 nmol/mgprot) and hydrogen peroxide (14.74 to 7.19 mmol/gprot) and restoring the level of total superoxide dismutase (24.32 to 42.55 U/mgprot) and glutathione (7.69 to 13.03 μg/mgprot). These results indicated that vitamin C could protect against triptolide-induced liver injury via reducing oxidative stress, and vitamin C may pose a significant health protection in the clinical use of triptolide.
DOI: 10.6084/m9.figshare.8324681.v1
title: Combined index of genomic prediction methods applied to productivity

description: ABSTRACT: Rice cultivation has great national and global importance, being one of the most produced and consumed cereals in the world and the primary food for more than half of the world’s population. Because of its importance as food, developing efficient methods to select and predict genetically superior individuals in reference to plant traits is of extreme importance for breeding programs. The objective of this research was to evaluate and compare the efficiency of the Delta-p, G-BLUP (Genomic Best Linear Unbiased Predictor), BayesCpi, BLASSO (Bayesian Least Absolute Shrinkage and Selection Operator), Delta-p/G-BLUP index, Delta-p/BayesCpi index, and Delta-p/BLASSO index in the estimation of genomic values and the effects of single nucleotide polymorphisms on phenotypic data associated with rice traits. Use of molecular markers allowed high selective efficiency and increased genetic gain per unit time. The Delta-p method uses the concept of change in allelic frequency caused by selection and the theoretical concept of genetic gain. The Index is based on the principle of combined selection, using the information regarding the additive genomic values predicted via G-BLUP, BayesCpi, BLASSO, or Delta-p. These methods were applied and compared for genomic prediction using nine rice traits: flag leaf length, flag leaf width, panicles number per plant, primary panicle branch number, seed length, seed width, amylose content, protein content, and blast resistance. Delta-p/G-BLUP index had higher predictive abilities for the traits studied, except for amylose content trait in which the method with the highest predictive ability was BayesCpi, being approximately 3% greater than that of the Delta-p/G-BLUP index.
DOI: 10.6084/m9.figshare.8324663
title: Synthesis of New Reconfigurable Limited Size FSS Structures Using an Improved Hybrid Particle Swarm Optimization

description: Abstract This paper describes a new design methodology for reconfigurable printed circuits with limited size, using an improved hybrid particle swarm optimization (HPSO) algorithm, reducing the search space by the definition of negative zones (NZ), regions where the swarm of particles should not travel. The proposed design methodology (HPSO-NZ) is used in the development of reconfigurable frequency selective surfaces (RFSSs), restricted to a limited overall size, resulting in entirely new frequency selective surface (FSS) geometries. Two FSS prototypes are designed, fabricated, and measured for comparison purpose. A good agreement is observed between simulation and measurements results, confirming the efficiency and accuracy of the HPSO-NZ algorithm. Also, the performance of the HPSO-NZ algorithm is compared to the ones of genetic algorithm (GA) and particle swarm optimization (PSO) algorithm, showing good consistency results.
DOI: 10.6084/m9.figshare.8324666
title: TRANSITION FROM SYSTEMATIC TO DIRECTED SOIL SAMPLING DESIGNS IN AN AREA MANAGED WITH PRECISION AGRICULTURE

description: ABSTRACT In agricultural areas with a historical of systematic soil sampling, alternative methodologies such as directed sampling design based on management zones (MZ) have been proposed to reduce sampling costs. The aim of this study was to evaluate the technical and economic impacts of replacing a dense systematic soil sampling design (cell size of 0.5 ha) by a systematic sampling with a smaller number of samples (cell size ranging from 1 to 4.5 ha), directed or conventional sampling design on the mapping of soil plant-available phosphorus (P), exchangeable potassium (K), and pHwater. The study was carried out in an agricultural area of 120 ha with soil classified as an Oxisol. The directed sampling designs were based on MZ delimited from data of elevation and overlapping of crop yield maps. Our finding revealed that systematic samplings with grids larger than 2 ha were not efficient to detect the spatial variability of soil P, K and pHwater. Larger systematic grid sizes, directed and conventional sampling design resulted in more generalist thematic maps, losing information about spatial variability of the soil attributes. Thus, from a technical point of view, soil sampling designs with a low density were little efficients, particularly for mapping P and K, due to their higher spatial variability. However, because soil P and K contents were close to or above critical levels and soil acidity was low (average pH close to 5.5), the different sampling designs presented little influence on fertilizer and liming recommendations. Therefore, we concluded that systematic soil sampling design may be replaced by soil sampling directed based on MZ or even by conventional sampling in soils with high fertility to reduce sampling costs. Nevertheless, crop responses must be monitored to validate fertilization management based on these simplifications on soil sampling procedure.
DOI: 10.6084/m9.figshare.8324663.v1
title: Synthesis of New Reconfigurable Limited Size FSS Structures Using an Improved Hybrid Particle Swarm Optimization

description: Abstract This paper describes a new design methodology for reconfigurable printed circuits with limited size, using an improved hybrid particle swarm optimization (HPSO) algorithm, reducing the search space by the definition of negative zones (NZ), regions where the swarm of particles should not travel. The proposed design methodology (HPSO-NZ) is used in the development of reconfigurable frequency selective surfaces (RFSSs), restricted to a limited overall size, resulting in entirely new frequency selective surface (FSS) geometries. Two FSS prototypes are designed, fabricated, and measured for comparison purpose. A good agreement is observed between simulation and measurements results, confirming the efficiency and accuracy of the HPSO-NZ algorithm. Also, the performance of the HPSO-NZ algorithm is compared to the ones of genetic algorithm (GA) and particle swarm optimization (PSO) algorithm, showing good consistency results.
DOI: 10.6084/m9.figshare.8324666.v1
title: TRANSITION FROM SYSTEMATIC TO DIRECTED SOIL SAMPLING DESIGNS IN AN AREA MANAGED WITH PRECISION AGRICULTURE

description: ABSTRACT In agricultural areas with a historical of systematic soil sampling, alternative methodologies such as directed sampling design based on management zones (MZ) have been proposed to reduce sampling costs. The aim of this study was to evaluate the technical and economic impacts of replacing a dense systematic soil sampling design (cell size of 0.5 ha) by a systematic sampling with a smaller number of samples (cell size ranging from 1 to 4.5 ha), directed or conventional sampling design on the mapping of soil plant-available phosphorus (P), exchangeable potassium (K), and pHwater. The study was carried out in an agricultural area of 120 ha with soil classified as an Oxisol. The directed sampling designs were based on MZ delimited from data of elevation and overlapping of crop yield maps. Our finding revealed that systematic samplings with grids larger than 2 ha were not efficient to detect the spatial variability of soil P, K and pHwater. Larger systematic grid sizes, directed and conventional sampling design resulted in more generalist thematic maps, losing information about spatial variability of the soil attributes. Thus, from a technical point of view, soil sampling designs with a low density were little efficients, particularly for mapping P and K, due to their higher spatial variability. However, because soil P and K contents were close to or above critical levels and soil acidity was low (average pH close to 5.5), the different sampling designs presented little influence on fertilizer and liming recommendations. Therefore, we concluded that systematic soil sampling design may be replaced by soil sampling directed based on MZ or even by conventional sampling in soils with high fertility to reduce sampling costs. Nevertheless, crop responses must be monitored to validate fertilization management based on these simplifications on soil sampling procedure.
DOI: 10.6084/m9.figshare.8324654
title: Evaluation of Gastro-protective Activity of Myristica fragrans on Ethanol-induced Ulcer in Albino Rats

description: Abstract: Myristica fragrans seeds are traditionally used to treat dyspepsia, vomiting and abdominal pain. The purpose of this study was to investigate the protective effect of Myristica fragrans in ethanol induced gastric ulcer. Study was carried out on rats, divided into four groups; negative control, positive control, standard drug control, and Myristica fragrans extract treated rats. The pH, ulcer index, acidity values and histopathological examination of stomach were evaluated. Myristica fragrans significantly (P&lt;0.05) reduced gastric lesions by 41.68% in ethanol induced ulcer model at 200 mg/kg when compared to sucralfate (60.41%). However, histopathological findings appeared similar in Myristica fragrans extract treated and standard drug control groups, where stomachs were found with mild erosion of superficial epithelium and few infiltrated inflammatory cells. pH of gastric contents of rats from extract treated was increased (4.25 ± 0.25) as compared to positive control group (2.25 ± 0.25). Ulcer index of extract treated rats was improved (41.74%). Moreover, total acidity of extract treated group (60.0 ± 0.47) was decreased as compared to control group (74.50 ± 1.04). It is concluded that Myristica fragrans showed significant protecting activity in ethanol induced ulcer. Isolation and purification of phytochemicals responsible for gastroprotective activity becomes necessary.