DOI: 10.5061/dryad.4j0zpc8fw
title: In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions

description: Mitochondria perform critical functions, including respiration, ATP
 production, small molecule metabolism, anti-oxidation, and they are
 involved in a number of human diseases. While the mitochondrial genome
 contains a small number of protein-coding genes, the vast majority of
 mitochondrial proteins are encoded by nuclear genes. In fission yeast,
 Schizosaccharomyces pombe, we screened 457 deletion (del) mutants
 deficient in nuclear-encoded mitochondrial proteins, searching for those
 that fail to form colonies in culture medium containing low glucose
 (0.03–0.1%; low-glucose sensitive, lgs), but that proliferate in regular
 2–3% glucose medium. Sixty-five (14%) of the 457 deletion mutants
 displayed the lgs phenotype. Thirty-three of them are defective either in
 dehydrogenases, subunits of respiratory complexes, the TCA cycle, or in
 one of the 9 steps of the CoQ10 biosynthetic pathway. The remaining 32 lgs
 mutants do not seem to be directly related to respiration. Fifteen are
 implicated in translation, and 6 encode transporters. The remaining 11
 function in anti-oxidation, amino acid synthesis, repair of DNA damage,
 microtubule cytoskeleton, intracellular mitochondrial distribution, or
 unknown functions. These 32 diverse lgs genes collectively maintain
 mitochondrial functions under low (1/20~1/60x normal) glucose
 concentrations. Interestingly, 30 of them have homologs associated with
 human diseases.
description: # In fission yeast, 65 non-essential mitochondrial proteins related to
 respiration and stress become essential in low-glucose conditions
 [https://doi.org/10.5061/dryad.4j0zpc8fw](https://doi.org/10.5061/dryad.4j0zpc8fw) These files include electronic supplementary materials for the article "In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions." In fission yeast, Schizosaccharomyces pombe, we screened 457 deletion mutants deficient in nuclear-encoded mitochondrial proteins, searching for those that fail to form colonies in culture medium containing low glucose (0.03-0.1%, low glucose sensitive, lgs), but that proliferate in regular 2-3% glucose medium. This dataset contains a list of deletion mutants screened in this study (Supplementary Table S2), a detailed list of genes required for cell proliferation in low glucose (Supplementary Table S1), and cell proliferation of the 10 selected lgs mutant strains under low glucose conditions (Supplementary Figure S1). ## Description of the data and file structure **Supplementary Figure S1. lgs deletion mutants fail to divide under low glucose conditions.** The cell number increase of wild-type (ED666, ED668) and representative lgs gene deletion mutant strains under low glucose conditions. Cells of these strains were cultivated in liquid YES medium, the glucose concentration of which was shifted from 2% to 0.08% at time 0. The rate of wild-type cell number increase was delayed, but recovered after 4 hr in the wild-type, whereas lgs mutants failed to recover the former rate of cell division. **Supplementary Table S1. S. pombe deletion mutants that exhibited the lgs phenotype.** Sixty-five lgs genes identified by screening 457 gene deletion mutants were classified according to their predicted functions. The systematic gene IDs are listed along with the common fission yeast gene names (Gene name), the lgs ranks determined by the glucose concentration below which the mutants failed to grow (rank A &lt;0.06%; rank B &lt;0.04%; rank C &lt; 0.03%) (LGS rank), the predicted gene products (Predicted gene product), the gene names of budding yeast and human homologs (Homologs in S. cerevisiae and Homologs in human), and a full list of diseases associated to the defects in human homologs of lgs genes (Associated disease). See Supplementary Table S2 for the full list of 457 gene deletion mutants examined in this study. **Supplementary Table S2. 457 gene deletion mutants examined in this study.** This table lists 457 gene deletion mutants examined in this study, which were classified according to their predicted functions of the products of the genes deleted. This table contains the common fission yeast gene names (Gene symbol), the systematic gene IDs (Systematic ID), and the predicted gene products (Product description). See Supplementary Table S1 for the 65 lgs genes, deletion of which led to defects in cell proliferation under low glucose conditions. ## Sharing/Access information A set of S. pombe deletion mutant strains (version 3) were purchased from Bioneer (Daejeon, Republic of Korea), and 457 strains that lack genes related to mitochondrial functions were selected, according to descriptions in the fission yeast genome database, Pombase (https://www.pombase.org/). See Supplementary Table S2 for the list of 457 gene deletion mutants examined in this study.