cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12189015.v1
title: Keratinous biomarker of mercury exposure associated with amyotrophic lateral sclerosis risk in a nationwide U.S. study

description: <i>Objective</i>: The majority of cases of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS) are of unknown etiology. A proportion of these cases are likely to be attributable to contaminant exposures, although the specific environmental etiology of ALS remains largely a mystery. Certain forms of the neurotoxic metal mercury readily cross into the central nervous system. Fish is a dietary source of methylmercury, but also of beneficial components, such as omega-3 polyunsaturated fatty acids. Prior work using clinic-based studies of toenails and hair as keratinous biomarkers of exposure suggest elevated mercury levels in ALS patients compared with controls. We sought to validate this relationship in a U.S. case-control comparison of mercury levels in nail clippings. <i>Methods</i>: We performed trace element analysis using inductively coupled plasma mass spectrometry (ICP-MS) on the nail clippings of <i>n</i> = 70 female, geographically representative ALS patients from the National ALS Biorepository and compared them to <i>n</i> = 210 age-matched controls from a set of <i>n</i> = 1216 nationally distributed controls from the Sister and Two Sister Studies. <i>Results</i>: Compared to the lowest quartile of nail mercury, moderate levels were associated with decreased risk of ALS (<i>P</i> = 4.18e–6). However, the odds of having nail mercury levels above the 90th percentile were 2.3-fold higher among ALS patients compared with controls (odds ratio (OR) = 2.3, 95% confidence interval 1.10–4.58, adjusted for age and smoking status). <i>Conclusion</i>: This finding suggests that excessive mercury exposure may be associated with the neurodegenerative health of aging populations.
DOI: 10.6084/m9.figshare.12199032.v1
title: Prevalence of <i>C9orf72</i> hexanucleotide repeat expansion in Greek patients with sporadic ALS

description: A total of 178 consecutive patients with definite sALS without frontotemporal dementia (FTD) were enrolled in this study, after complete clinical evaluation. A Repeat-Primed Polymerase Chain Reaction (RP-PCR) protocol was applied to detect the G<sub>4</sub>C<sub>2</sub> repeats expansions. In the studied sALS patients, 5.06% (<i>n</i> = 9) carried the <i>C9orf72</i> mutation. Among carriers, 2/3 of them were females and spinal onset accounted for 78% and bulbar for 22%, while the mean age of onset was about 60 years. Our study showed that the prevalence of <i>C9orf72</i> repeat expansion in Greek sALS patients is similar to the overall frequency of the mutation in European populations. The pathogenic mutation remains a promising biomarker for genetic testing and targeted treatment.
DOI: 10.6084/m9.figshare.12189015
title: Keratinous biomarker of mercury exposure associated with amyotrophic lateral sclerosis risk in a nationwide U.S. study

description: <i>Objective</i>: The majority of cases of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS) are of unknown etiology. A proportion of these cases are likely to be attributable to contaminant exposures, although the specific environmental etiology of ALS remains largely a mystery. Certain forms of the neurotoxic metal mercury readily cross into the central nervous system. Fish is a dietary source of methylmercury, but also of beneficial components, such as omega-3 polyunsaturated fatty acids. Prior work using clinic-based studies of toenails and hair as keratinous biomarkers of exposure suggest elevated mercury levels in ALS patients compared with controls. We sought to validate this relationship in a U.S. case-control comparison of mercury levels in nail clippings. <i>Methods</i>: We performed trace element analysis using inductively coupled plasma mass spectrometry (ICP-MS) on the nail clippings of <i>n</i> = 70 female, geographically representative ALS patients from the National ALS Biorepository and compared them to <i>n</i> = 210 age-matched controls from a set of <i>n</i> = 1216 nationally distributed controls from the Sister and Two Sister Studies. <i>Results</i>: Compared to the lowest quartile of nail mercury, moderate levels were associated with decreased risk of ALS (<i>P</i> = 4.18e–6). However, the odds of having nail mercury levels above the 90th percentile were 2.3-fold higher among ALS patients compared with controls (odds ratio (OR) = 2.3, 95% confidence interval 1.10–4.58, adjusted for age and smoking status). <i>Conclusion</i>: This finding suggests that excessive mercury exposure may be associated with the neurodegenerative health of aging populations.
DOI: 10.6084/m9.figshare.12199032
title: Prevalence of <i>C9orf72</i> hexanucleotide repeat expansion in Greek patients with sporadic ALS

description: A total of 178 consecutive patients with definite sALS without frontotemporal dementia (FTD) were enrolled in this study, after complete clinical evaluation. A Repeat-Primed Polymerase Chain Reaction (RP-PCR) protocol was applied to detect the G<sub>4</sub>C<sub>2</sub> repeats expansions. In the studied sALS patients, 5.06% (<i>n</i> = 9) carried the <i>C9orf72</i> mutation. Among carriers, 2/3 of them were females and spinal onset accounted for 78% and bulbar for 22%, while the mean age of onset was about 60 years. Our study showed that the prevalence of <i>C9orf72</i> repeat expansion in Greek sALS patients is similar to the overall frequency of the mutation in European populations. The pathogenic mutation remains a promising biomarker for genetic testing and targeted treatment.
DOI: 10.15129/523764a3-e528-4fda-a7d0-02d9a553f67a
title: Appendices for PhD Thesis: "Laboratory insights into reactions between gas shales and hydraulic fracturing fluids at reservoir temperatures and pressures: a global perspective."

description: Three excel spredsheets contain extended geochemical data produced as part of the PhD research and referenced in the thesis. The data was generated using ICP-MS and ICP-AES at external laboratories. Appendix A - contains total recoverable element concentrations (TRE sheet) and acid extractable element concentrations (AEE sheet) for approximately 65 elements for all the shale samples used in this study. The element concentrations are reported as an average of duplicates. Appendix B - contains five sheets as follows with element concentrations reported as an average of duplicates: Table B1 SHFF: Summary results of leaching tests with synthetic hydraulic fracturing fluid (SHFF) for elements of interest. Table B2 SGW: Summary results of leaching tests with synthetic groundwater (SGW) for elements of interest. Table B3 % SHFF TRE: SHFF leaching results for elements of interest, normalised to the element content of each sample (TRE) and expressed as a %. Numbers in red are % calculated based on the detection limit. Table B4 % TRE SGW: SGW leaching results for elements of interest, normalised to the element content of each sample (TRE) and expressed as a %. Numbers in red are % calculated based on the detection limit. Table B5 1M HCl: results of the preliminary 1M HCl leaching tests. % TRE represents element concentrations normalised to a given element total recoverable content of each sample. Numbers in red are % calculated based on the detection limit. Appendix C - a full dataset of the synthetic hydraulic fracturing fluid (SHFF, 54 elements) and synthetic groundwater (SGW, 54 elements) leaching results presented in Chapter 4.4. The element concentrations are reported as an average of duplicates.
DOI: 10.25646/9100
title: Tagesdaten-CSV aus dem DIVI-Intensivregister

description: Die Tagesdaten-CSV entspricht einem Auszug der Daten des DIVI-Intensivregisters. Die Datei enthält eine Aggregation der aktuellsten Meldungen pro Landkreis. Es werden die aktuell gemeldeten Anzahlen der COVID-19 Intensivfälle sowie die gemeldeten intensivmedizinischen Behandlungskapazitäten angezeigt. Die Tagesdaten-CSV liefert dabei ausschließlich einen Blick auf die Daten gemäß dem Stand des betrachteten Tages. Die Daten sind im situationsbedingten Kontext aufbereitet, damit sind verschiedene Tagesdaten-CSVs u.U. nicht direkt miteinander vergleichbar. Die aktuellsten Meldungen werden im gewählten Betrachtungszeitfenster über alle Meldebereiche und Standorte aufsummiert. Weitere Informationen sind zu finden unter https://www.intensivregister.de/#/faq
DOI: 10.15129/d5b80e9f-2f49-45d6-9432-c23470f4e851
title: Amikacin data for population pharmacokinetic modelling

description: Anonymised patient data extracted from routine clinical files between January 2002 and February 2018. Data were extracted by Fiona Robb (NHS antimicrobial pharmacist) and collated by Hinke Siebinga (student) and Alison Thomson (Senior Lecturer) As the data were generated in the course of routine clinical practice and fully anonymized, the study was judged by the NHS as a service evaluation for which patient consent and formal ethics approval were not required. Caldicott Guardian approval was obtained for data extraction. The data file is an Excel spreadsheet formatted for population pharmacokinetic analysis containing amikacin doses, rates and times of administration
DOI: 10.15129/e90e0b48-d5f4-45ab-81d1-1ce9d3031719
title: Amoxicillin, metronidazole and gentamicin data from patients undergoing colorectal surgery

description: Anonymised patient characteristics, dose and concentration data from a study of amoxicillin, metronidazole and gentamicin dosage regimens for use in antibiotic prophylaxis in colorectal surgery
DOI: 10.5281/zenodo.5534704
title: Living room environment

description: An audio-visual model for virtual reality of the living room laboratory at the CvO university Oldenburg. The model has been created for a highly accurate acoustic rendering over loudspeaker arrays and headphones and for visual representation in virtual reality. The package encompasses an accurate spatial geometric model of the living room and adjacent kitchen, which can be used for acoustic simulations, and a visual model for virtual reality. It further includes a definition of two acoustic scenes for the purpose of audiology and hearing research together with the associated measurements of the acoustic impulse responses using a binaural recording system (binaural room impulse responses), a spherical microphone array, and omnidirectional microphones.
description: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 352015383 – SFB 1330, Project C4 and C5.
DOI: 10.5281/zenodo.5535827
title: Supplementary material to 'New Equivalent Frame Models for Historical Masonry Structures'

description: This repository provides the OpenSEES models used for producing the results presented in the paper as well as the data with results of the IDAs.
DOI: 10.5281/zenodo.5535826
title: Supplementary material to 'New Equivalent Frame Models for Historical Masonry Structures'

description: This repository provides the OpenSEES models used for producing the results presented in the paper as well as the data with results of the IDAs.
DOI: 10.3886/e151121
title: HIgh Stakes Trust Game with Physiology

description: Trust Game with pre-decision face to face interactions, high stakes, and neurophysiological measures.
DOI: 10.3886/e151121v1
title: HIgh Stakes Trust Game with Physiology

description: Trust Game with pre-decision face to face interactions, high stakes, and neurophysiological measures.
DOI: 10.3886/e147581
title: Oxytocin Release Increases with Age and is Associated with Life Satisfaction and Prosocial Behaviors

description: Full dataset for this paper
DOI: 10.3886/e147581v1
title: Oxytocin Release Increases with Age and is Associated with Life Satisfaction and Prosocial Behaviors

description: Full dataset for this paper
DOI: 10.6084/m9.figshare.1598222
title: Defining the Relationships Among Retinal Function, Layer Thickness and Visual Behavior During Oxidative Stress-Induced Retinal Degeneration

description: <i>Purpose</i>: The purpose of this study was to identify how changes in retinal structure and function correlate with visual deficits during increasing amounts of retinal degeneration. <i>Materials and methods</i>: Retinal degeneration was induced in adult mice by subretinal injections of paraquat (PQ) (0.2–1 mM). Retinal anatomy and photoreceptor layer thickness were quantified by histology and optical coherence tomography (OCT), retinal function was measured using electroretinography (ERG), and visual behavior were measured by optokinetic tracking, at 1 to 3 week post-injury. <i>Results</i>: Photoreceptor layer structure, function and visual behavior declined at a linear rate over time following PQ-induced degeneration, with the correlations between outcome measures being lowest at mild injury levels and increasing with injury severity. Overall reductions in visual acuity were highly correlated with declines in retinal thickness (<i>r</i><sup>2</sup> = 0.78) and function (<i>r</i><sup>2</sup> = 0.67) and retinal thickness correlated with photoreceptor function (<i>r</i><sup>2</sup> = 0.72). ERG a-wave scotopic amplitudes showed a stronger correspondence to retinal structure and visual behavior than b-waves. <i>Conclusions</i>: Measurements of photoreceptor loss at the structural and functional levels showed good correspondence with degeneration-associated changes in visual behavior after oxidative stress injury. The results provide new insight about the relative kinetics of measurements of retinal degeneration induced by oxidative stress, which could guide the choice of optimal outcome measurements for other retinal diseases.
DOI: 10.6084/m9.figshare.1598222.v4
title: Defining the Relationships Among Retinal Function, Layer Thickness and Visual Behavior During Oxidative Stress-Induced Retinal Degeneration

description: <i>Purpose</i>: The purpose of this study was to identify how changes in retinal structure and function correlate with visual deficits during increasing amounts of retinal degeneration. <i>Materials and methods</i>: Retinal degeneration was induced in adult mice by subretinal injections of paraquat (PQ) (0.2–1 mM). Retinal anatomy and photoreceptor layer thickness were quantified by histology and optical coherence tomography (OCT), retinal function was measured using electroretinography (ERG), and visual behavior were measured by optokinetic tracking, at 1 to 3 week post-injury. <i>Results</i>: Photoreceptor layer structure, function and visual behavior declined at a linear rate over time following PQ-induced degeneration, with the correlations between outcome measures being lowest at mild injury levels and increasing with injury severity. Overall reductions in visual acuity were highly correlated with declines in retinal thickness (<i>r</i><sup>2</sup> = 0.78) and function (<i>r</i><sup>2</sup> = 0.67) and retinal thickness correlated with photoreceptor function (<i>r</i><sup>2</sup> = 0.72). ERG a-wave scotopic amplitudes showed a stronger correspondence to retinal structure and visual behavior than b-waves. <i>Conclusions</i>: Measurements of photoreceptor loss at the structural and functional levels showed good correspondence with degeneration-associated changes in visual behavior after oxidative stress injury. The results provide new insight about the relative kinetics of measurements of retinal degeneration induced by oxidative stress, which could guide the choice of optimal outcome measurements for other retinal diseases.
DOI: 10.6084/m9.figshare.1598222.v3
title: Defining the Relationships Among Retinal Function, Layer Thickness and Visual Behavior During Oxidative Stress-Induced Retinal Degeneration

description: <i>Purpose</i>: The purpose of this study was to identify how changes in retinal structure and function correlate with visual deficits during increasing amounts of retinal degeneration. <i>Materials and methods</i>: Retinal degeneration was induced in adult mice by subretinal injections of paraquat (PQ) (0.2–1 mM). Retinal anatomy and photoreceptor layer thickness were quantified by histology and optical coherence tomography (OCT), retinal function was measured using electroretinography (ERG), and visual behavior were measured by optokinetic tracking, at 1 to 3 week post-injury. <i>Results</i>: Photoreceptor layer structure, function and visual behavior declined at a linear rate over time following PQ-induced degeneration, with the correlations between outcome measures being lowest at mild injury levels and increasing with injury severity. Overall reductions in visual acuity were highly correlated with declines in retinal thickness (<i>r</i><sup>2</sup> = 0.78) and function (<i>r</i><sup>2</sup> = 0.67) and retinal thickness correlated with photoreceptor function (<i>r</i><sup>2</sup> = 0.72). ERG a-wave scotopic amplitudes showed a stronger correspondence to retinal structure and visual behavior than b-waves. <i>Conclusions</i>: Measurements of photoreceptor loss at the structural and functional levels showed good correspondence with degeneration-associated changes in visual behavior after oxidative stress injury. The results provide new insight about the relative kinetics of measurements of retinal degeneration induced by oxidative stress, which could guide the choice of optimal outcome measurements for other retinal diseases.
DOI: 10.6084/m9.figshare.5414746.v2
title: Maximized nanodrug-loaded mesenchymal stem cells by a dual drug-loaded mode for the systemic treatment of metastatic lung cancer

description: Mesenchymal stem cells (MSCs), exhibiting tumor-tropic and migratory potential, can serve as cellular carriers to improve the effectiveness of anticancer agents. However, several challenges, such as the safety issue, the limited drug loading, the conservation of stemness and migration of MSCs, still remain in the MSC-based delivery system. In the present study, a novel nano-engineered MSC delivery system was established by loading doxorubicin (DOX)–polymer conjugates for the systemic treatment of pulmonary metastasis of breast cancer. For the first time, a dual drug-loaded mode, endocytosis and membrane-bound, was adopted to achieve the maximum amount of DOX conjugates in MSCs. The <i>in vitro</i> studies revealed the loaded MSCs possessed multifunctional properties, including preservation of the stemness and migration of MSCs, excellent stability of drug loading, acid sensitive drug release and obvious cytotoxicity against 4T1 cells. The <i>in vivo</i> studies confirmed that the loaded MSCs mainly located and long stayed in the lung where the foci of metastatic tumor situated. Importantly, loaded MSCs can significantly inhibit the tumor growth and prolong the life span of tumor-bearing mice in contrast with DOX and DOX-conjugate. The present loaded MSCs system suggested a promising strategy to solve several issues existed in cell-based delivery systems. Especially for the problem of low drug loading, the strategy, simultaneously loading nanodrug in cells’ internal and membrane, might be the most desirable method so far and could be developed as a generalizable manner for cell-mediated tumor-targeted therapy.
DOI: 10.6084/m9.figshare.5350375.v1
title: Novel reverse electrodialysis-driven iontophoretic system for topical and transdermal delivery of poorly permeable therapeutic agents

description: Topical and transdermal drug delivery has great potential in non-invasive and non-oral administration of poorly bioavailable therapeutic agents. However, due to the barrier function of the stratum corneum, the drugs that can be clinically feasible candidates for topical and transdermal delivery have been limited to small-sized lipophilic molecules. Previously, we fabricated a novel iontophoretic system using reverse electrodialysis (RED) technology (RED system). However, no study has demonstrated its utility in topical and/or transdermal delivery of poorly permeable therapeutic agents. In this study, we report the topical delivery of fluorescein isothiocyanate (FITC)–hyaluronic acid (FITC–HA) and vitamin C and the transdermal delivery of lopinavir using our newly developed RED system in the <i>in vitro</i> hairless mouse skin and <i>in vivo</i> Sprague–Dawley rat models. The RED system significantly enhanced the efficiency of topical HA and vitamin C and transdermal lopinavir delivery. Moreover, the efficiency and safety of transdermal delivery using the RED system were comparable with those of a commercial ketoprofen patch formulation. Thus, the RED system can be a potential topical and transdermal delivery system for various poorly bioavailable pharmaceuticals including HA, vitamin C, and lopinavir.
DOI: 10.6084/m9.figshare.14798289
title: Average Nucleotide Identity of 40 Agrobacterium spp.

description: Average Nucleotide Identity of 40 Agrobacterium spp. computed with pyANI
DOI: 10.6084/m9.figshare.5580259.v2
title: A novel lipid prodrug strategy for sustained delivery of hexadecyloxypropyl 9-[2-(phosphonomethoxy)ethyl]guanine (HDP-PMEG) on unwanted ocular proliferation

description: Proliferative vitreoretinopathy (PVR) is a blinding eye disease and there is no effective pharmacological measure to prevent PVR development. The difficulty comes from lack of potent antiproliferative agent and lack of sustained delivery to cover high-risk time window for PVR to develop. Lipid prodrug of PMEG, hexadecyloxypropyl 9-[(2-phosphonomethoxy)ethyl]guanine (HDP-PMEG), was prepared and was evaluated as a pharmacological adjuvant to surgical management of PVR. A dose-escalation study determined that the highest nontoxic dose for intravitreal use in pigmented rabbits was 3 µg per eye. The genotoxicity of HDP-PMEG was harnessed as a perioperative preventative measure against PVR in a rabbit eye model while the sustained intravitreal pharmacological effect was evaluated on a laser-induced fibrovascular model in rat eye. After intravitreal 3 µg, HDP-PMEG particles in the rabbit vitreous was visible for at least 6 weeks. A single 50-min intravitreal infusion of HDP-PMEG demonstrated significant inhibition of PVR formation when compared with the eyes infused with only BSS (BSS vs. HDP-PMEG: estimate = 1.14, OR = 3.1, <i>p</i> = .027). A single intravitreal 104 ng (equivalent to 3 µg for rabbit eye) of HDP-PMEG significantly inhibit laser-induced fibrovascular proliferation in rat eye by 55% (least square mean pixel, BSS = 4763569.5 vs. HDP-PMEG = 2148129.7, <i>p</i> p
DOI: 10.6084/m9.figshare.5580259.v1
title: A novel lipid prodrug strategy for sustained delivery of hexadecyloxypropyl 9-[2-(phosphonomethoxy)ethyl]guanine (HDP-PMEG) on unwanted ocular proliferation

description: Proliferative vitreoretinopathy (PVR) is a blinding eye disease and there is no effective pharmacological measure to prevent PVR development. The difficulty comes from lack of potent antiproliferative agent and lack of sustained delivery to cover high-risk time window for PVR to develop. Lipid prodrug of PMEG, hexadecyloxypropyl 9-[(2-phosphonomethoxy)ethyl]guanine (HDP-PMEG), was prepared and was evaluated as a pharmacological adjuvant to surgical management of PVR. A dose-escalation study determined that the highest nontoxic dose for intravitreal use in pigmented rabbits was 3 µg per eye. The genotoxicity of HDP-PMEG was harnessed as a perioperative preventative measure against PVR in a rabbit eye model while the sustained intravitreal pharmacological effect was evaluated on a laser-induced fibrovascular model in rat eye. After intravitreal 3 µg, HDP-PMEG particles in the rabbit vitreous was visible for at least 6 weeks. A single 50-min intravitreal infusion of HDP-PMEG demonstrated significant inhibition of PVR formation when compared with the eyes infused with only BSS (BSS vs. HDP-PMEG: estimate = 1.14, OR = 3.1, <i>p</i> = .027). A single intravitreal 104 ng (equivalent to 3 µg for rabbit eye) of HDP-PMEG significantly inhibit laser-induced fibrovascular proliferation in rat eye by 55% (least square mean pixel, BSS = 4763569.5 vs. HDP-PMEG = 2148129.7, <i>p</i> p
DOI: 10.6084/m9.figshare.14798289.v2
title: Average Nucleotide Identity of 40 Agrobacterium spp.

description: Average Nucleotide Identity of 40 Agrobacterium spp. computed with pyANI