cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0p55mfj
title: CIL:36665, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The first culture of P. multimicronucleatum which I had obtained from Carolina Biological Supply House contained a bacterial endosymbiont that resided specifically in the space between the two membranes of the nuclear envelope or in outpocketings of this space so that the bacteria were enclosed by the outer membrane of the nuclear envelope. Such endosymbionts have been observed before (Jenkins, J. Gen. Microbiol. 61:355-359, 1970). In this micrograph the bacteria are associated with the micronucleus, which has a remnant bundle of fibers and microtubules, but bacteria are also associated with the macronucleus (not shown). TEM taken on 12/9/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.2µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0vq31bg
title: CIL:36728, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Glutaraldehyde fixation followed by Gomori acid phosphatase treatment and dehydration in alcohol and embedding in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: To see if acidosomes contain hydrolases (digestive enzymes) we incubated cells in Gomori‚Äôs medium which in the presence of acid phosphatase and lead will form an electron-opaque reaction product. Acidosomes surrounding a DV-I are free of reaction product even though a nearby DV, presumably a DV-III, contains abundant reaction product. A few vesicles assumed to be lysosomes also contain some reaction product. This experiment demonstrated that acidosomes are not lysosomes and do not contain digestive enzymes at least not AcPase. TEM taken on 4/8/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm.
description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j018354h
title: CIL:36726, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: At the same time that the acidosomes fuse with the phagosome, the DV-I membrane, that originated from the discoidal vesicles, is transformed into tubules that are removed from the phagosome. By this process the DV-I is transformed from a phagosome into a phagoacidosome (DV-II), a process of apparently ‚Äútotal‚Äù membrane replacement. (For discussion see Allen et al., J. Cell Sci. 108:1263-1274, 1995). The mAb to antigen Q2 specifically labels the tubules that are being removed from the DV-I. TEM taken on 9/18/93 by R. Allen with Zeiss 10A TEM. Neg. 12,000X. Bar = 0.5µm. Part published in J. Cell Sci. 108:1263-1274, 1995. Adapted with permission.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40558) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0sj1jgc
title: CIL:36714, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Two of the three types of vesicles that are transported to the cytopharynx and that accumulate in large numbers at the cytopharynx are shown in this electron micrograph. The discoidal vesicles move up to the edge of the cytopharynx membrane where the cytopharynx membrane is continuous with the plasma membrane of the buccal cavity. Here the discoidal vesicles fuse with the cytopharynx membrane. Just behind this edge is found a layer of larger vesicles, the acidosomes, which dock with the cytopharynx membrane as soon as they make contact with this membrane. Unlike the discoidal vesicles these acidosomes do not fuse with the cytopharynx or nascent vacuole until the phagosome has pinched off and moved toward the posterior pole of the cell. TEM taken on 4/8/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,000X. Bar = 0.5µm.
description: After a light glutaraldehyde fixation, these organisms were incubated in Gomori reagent to reveal the presence of acid phosphatase then dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0v123ph
title: CIL:36713, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The postoral fibers are also heavily labeled by anti-b-tubulin. The cell was lightly fixed, placed in metylcellulose and frozen in liquid nitrogen. Sections were produced at -100¬∞C before thawing and treated with the b-tubulin antibody followed by immunogold labeled antibody to IgG. TEM taken on 2/25/94 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0g73cmz
title: CIL:36712, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: The cytopharyngeal ribbons of microtubules tend to curve into a ‚ÄúC‚Äù shape away from the cytopharynx but they still remain connected as a ribbon and are heavily labeled by immunogold when exposed to anti-b-tubulin. TEM taken on 2/27/94 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm.
DOI: 10.6075/j0bg2mxp
title: CIL:36645, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Like other ciliates P. multimicronucleatum has two morphologically and functionally different nuclei. Shown here is the tiny micronucleus, of which there are four, during interphase of this cell. This nucleus has the task of dividing the nuclear genome between the two daughter cells during mitosis and in forming the two haploid pronuclei one of which will be exchanged with a partner cell during conjugation. However, during interphase this nucleus is not involved in forming ribosomes or messenger RNA so it does not control the metabolic processes of the cell. This micronucleus, much smaller than in other paramecia such as P. caudatum, has a central condensed mass of chromatin surrounded by a less dense nucleoplasm. This is known as a vesicular type of micronucleus. There is a typical nuclear envelope with numerous pores. This micronucleus was from a cell in day 5 of a culture age study where cells are in mid-log phase of growth. The cell is presumably undergoing rapid metabolism. TEM taken on 5/29/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm.
DOI: 10.6075/j0jq1001
title: CIL:36643, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: QF-DE of a crystal whose P-fracture face shows many IMPs similar to the P-face of an acidosome lying nearby and trichocyst as well as trichocyst precursor vesicles. TEM taken by C. Schroeder on 6/3/88 with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm.
DOI: 10.6075/j0cj8cfs
title: CIL:36642, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Quick-freeze deep-etch image of a crystal. The contents of the crystal have condensed into paracrystalline planes that break into angular chunks when fractured. A portion of the P-fracture face of the membrane has many pits where IMPs have been removed. This resembles the P-face of the phagolysosome membrane or stage III of digestive vacuoles. TEM taken on 4/6/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0bv7fkt
title: CIL:36698, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The postoral microtubular bundles form a track along which a newly released food vacuole rapidly migrates to the cell‚Äôs posterior end. Here the flattened cytopharyngeal membrane lies next to these bundles. TEM taken on 2/29/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j03x85kd
title: CIL:36697, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: At the posterior end of the buccal cavity the right side of the cytopharynx can be seen where it meets the left side. The right side has a groove in which microtubules lie parallel to the long axis of the groove and are bound to the membrane along their length. The ends of the ribbed wall lie ventral to this groove and have a filamentous reticulum covering the ribs. Bundles of postoral microtubules border the ribs and extend far into the cytosol. TEM taken on 2/29/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,500X. Bar = 0.5µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0gh9gwt
title: CIL:36696, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: The buccal cavity opens into the developing digestive (food) vacuole at the terminal edges of the alveolar sacs that cover the buccal cavity. A plane connecting these edges would roughly describe a tear drop-shaped opening that has been called the cytostome or cell mouth. The cytopharynx (cp) just beyond the buccal cavity encircles the cytostome. The cytopharynx consists of two sides, the left side (shown here) from which 40 or more ribbons of cytopharyngeal microtubules curve over a cytostomal cord. This edge runs along the dorsal side of the quadrulus and borders the terminal margins of the alveolar sacs. Discoidal vesicles are transported along the microtubular ribbons to the left edge of the cytopharynx. TEM taken on 3/25/81 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm
DOI: 10.6075/j0pc3191
title: CIL:36694, Danio rerio, early embryonic cell. In Cell Image Library

description: Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; distribution of epitope
Visualization methods: EGFP; phalloidin; hoescht
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Zebrafish embryo undergoing cytokinesis. The embryo is expressing fixed EMTB-3GFP (microtubules-marker, green) and is labeled with phalloidin (actin marker, red) and Hoechst (DNA marker, blue). Note the radial organization of microtubules
description: Image collected on an LSM 710 using a 20X 0.8NA objective.
DOI: 10.6075/j0n58k95
title: CIL:36706, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: The cytopharynx in cross section showing the cytopharyngeal microtubular ribbons lying in the plane of the section. Some discoidal vesicles, some containing HRP label, are also seen in planar view. Acidosomes also accumulate near the growing nascent digestive vacuole. The lower ribbon has 10 microtubules showing while, what are probably two ribbons lying side by side, show 22 microtubules in the upper ribbon. A normal sized discoidal vesicle lying flat against a ribbon will overlap with at least 6 microtubules. A strong association of a tubular vesicle with several microtubules probably spreads the tubule into the very regular discoidal shape. The arrow points to the sharp bend in the accessory microtubules coming from the edge of the quadrulus where they contact the filamentous reticulum. TEM taken on 4/18/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µm.