DOI: 10.5061/dryad.rs245
title: Data from: The effect of sex on the repeatability of evolution in different environments

description: The adaptive function of sex has been extensively studied, while less
 consideration has been given to the potential downstream consequences of
 sex on evolution. Here, we investigate one such potential consequence, the
 effect of sex on the repeatability of evolution. By affecting the
 repeatability of evolution, sex could have important implications for
 biodiversity, and for our ability to make predictions about the outcome of
 environmental change. We allowed asexual and sexual populations of
 Chlamydomonas reinhardtii to evolve in novel environments and monitored
 both their change in fitness and variance in fitness after evolution. Sex
 affected the repeatability of evolution by changing the importance of the
 effect of selection, chance, and ancestral constraints on the outcome of
 the evolutionary process. In particular, the effects of sex were highly
 dependent on the initial genetic composition of the population and on the
 environment. Given the lack of a consistent effect of sex on repeatability
 across the environments used here, further studies to dissect in more
 detail the underlying reasons for these differences as well as studies in
 additional environments are required if we are to have a general
 understanding of the effects of sex on the repeatability of evolution.
description: OD readings taken during
 the selection experimentThis file contains the
 optical density ("absorbance") measurements made at the end of
 each asexual and each sexual cycle during the course of the selection
 experiment. "Reproduction": mode of reproduction of the
 experimental line; "Environment": environment of selection;
 "History": genetic history of the experimental line (3 levels);
 "Cycle": wether the absorbance was measured at the end of an
 asexual cycle or at the end of a sexual cycle; "Time": cycle
 numberOD_readings.csvOD readings taken during the ancestral fitness assayThis file contains the optical density ("absorbance") measurements of each culture taken during the fitness assay of the ancestral spores. "Environment": assay environment; "History": genetic history of the ancestral spore (3 levels); "Spore": number identifying the spore; "Mt": mating type of the spore; "Replicate": replicate; "Time": time in days; "corrOD": absorbance corrected by the optical density of a well containing media but no cells; "corrLOG10N": the log10 value of the corrected OD measurementOD_readings_ancestral_fitness_assay.csvOD readings taken during the fitness assay of lines evolved in BoldsThis file contains the optical density ("absorbance") measurements of each culture taken during the fitness assay of the lines evolved in Bold's. "Selection": whether the spore assayed is ancestral or evolved; "History": the genetic history of the lines (3 levels); "Reproduction": the mode of reproduction of the lines during the selection experiment; "Line": number identifying the line; "Spore": number identifying the spore; "Replicate": replicate culture; "Time": time in days; "corrOD": the optical density value corrected by the OD value of a well with media only; "corrLOG10N": the log10 value of the corrected OD value.OD_readings_evolved_fitness_assay_Bolds.csvOD readings taken during the fitness assay of the lines evolved in HerbicidesThis file contains the optical density ("absorbance") measurements of each culture taken during the fitness assay of the lines evolved in Herbicides. "Selection": whether the spore assayed is ancestral or evolved; "History": the genetic history of the lines (3 levels); "Reproduction": the mode of reproduction of the lines during the selection experiment; "Line": number identifying the line; "Spore": number identifying the spore; "Replicate": replicate culture; "Time": time in days; "corrOD": the optical density value corrected by the OD value of a well with media only; "corrLOG10N": the log10 value of the corrected OD value.OD_readings_evolved_fitness_assay_Herbicides.csvOD readings taken during fitness assay of lines evolved NaClThis file contains the optical density ("absorbance") measurements of each culture taken during the fitness assay of the lines evolved in NaCl. "Selection": whether the spore assayed is ancestral or evolved; "History": the genetic history of the lines (3 levels); "Reproduction": the mode of reproduction of the lines during the selection experiment; "Line": number identifying the line; "Spore": number identifying the spore; "Replicate": replicate culture; "Time": time in days; "corrOD": the optical density value corrected by the OD value of a well with media only; "corrLOG10N": the log10 value of the corrected OD value.OD_readings_evolved_fitness_assay_NaCl.csvOD readings taken during fitness assay of lines evolved inNa2SO4This file contains the optical density ("absorbance") measurements of each culture taken during the fitness assay of the lines evolved in Na2SO4. "Selection": whether the spore assayed is ancestral or evolved; "History": the genetic history of the lines (3 levels); "Reproduction": the mode of reproduction of the lines during the selection experiment; "Line": number identifying the line; "Spore": number identifying the spore; "Replicate": replicate culture; "Time": time in days; "corrOD": the optical density value corrected by the OD value of a well with media only; "corrLOG10N": the log10 value of the corrected OD value.OD_readings_evolved_fitness_assay_Na2SO4.csvgrowth parameters of ancestral sporesThis file contains the estimates of growth parameters (maximum growth rate) for the ancestral spores. The growth parameters were estimated by fitting growth models onto the OD readings from the fitness assay. "Environment": the environment in which maximum growth rate was measured; "History": the genetic history of the lines (3 levels); "Spore": number identifying the spore; "Replicate": replicate culture; "Mt": mating type of the spore; "lag", "mumax", "log10N0", "log10Nmax": growth parameters; "growth_model": the model that was fitted to the data to estimate the growth parametersgrowth_parameters_ancestral_fitness_assay.csvgrowth parameters of evolved and ancestral spores from evolved fitness assayThis file contains the estimates of growth parameters (maximum growth rate) for the evolved lines and fastest growing ancestral spores. The growth parameters were estimated by fitting growth models onto the OD readings from the fitness assay. "Environment": the environment in which maximum growth rate was measured; "Selection": whether the spore is ancestral or evolved; "History": the genetic history of the lines (3 levels); "Reproduction": the mode of reproduction of the lines during the selection experiment; "Line": number identifying the experimental line; "Spore": number identifying the spore; "Replicate": replicate culture; "Mt": mating type of the spore; "lag", "mumax", "log10N0", "log10Nmax": growth parameters; "growth_model": the model that was fitted to the data to estimate the growth parametersgrowth.parameters.evolved.fitness.assay.csv