cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j03j3bx8
title: CIL:36243, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The new oral region in the opisthe starts to form as an unorganized field of new basal bodies develops at the equator of the cell surface. At first these do not resemble membranelles although some basal bodies already have short cilia. TEM taken on 8/8/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39515) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j09w0d8r
title: CIL:40660, Rattus, hippocampal neuron. In Cell Image Library

description: Preparation: detergent permeabilized; glutaraldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: dispersed cells in vitro
Item type: grey scale graphic
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: colorized
description: Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Red asterisks indicate putative PSD fragments. Yellow boxes are enlarged in panels with corresponding numbers. Box 1, interaction of putative PSD (arrow) from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Boxes 2 and 3, branched actin filaments (cyan) in the head (2) and at the neck‚Äìhead junction (3). Insets in panels 2 and 3 show nonpseudocolored regions outlined by yellow boxes. Dashed arrows in panel 3 indicate potential filament breakage. Bars, 0.2 μm. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.
description: Please refer to referenced article for details on cell extraction and processing for electron microscopy.
DOI: 10.6075/j01g0m92
title: CIL:36157, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Synapse development in cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (green) and the presynaptic vesicle protein Synapsin I (red) to show the density of presynaptic contacts along the dendritic arbor.
description: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (monoclonal HM2, Sigma, with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and synapsin I (from P. DeCamilli, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]). Images were acquired with a Leica DMRA microscope with a 20X objective (Fluotar, 0.5NA), Photometrics CoolSnap ES CCD camera and MetaMorph software. This color was generated using the MetaMorph "color combine" function.
DOI: 10.6075/j0qz28vw
title: CIL:35725, Saccharomyces cerevisiae. In Cell Image Library

description: Images were acquired using a 60x 1.42 NA objective lens on a Delta Vision system equipped with a CoolSNAP_HQ2 / HQ2-ICX285. Please read the microscopy section of the referenced paper for details before image analysis.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: This is a time lapse image series of Saccharomyces cerevisiae expressing GFP tagged Bub2, a GTPase activator involves in mitotic cell cycle spindle orientation checkpoint and regulation of exit from mitosis. These phase and GFP images are part of an image group containing CIL 35720-35739. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.
DOI: 10.6075/j008644x
title: CIL:39050, lung cancer cell. In Cell Image Library

description: Colorized scanning electron micrograph of a lung cancer cell grown in culture.
description: B0007656 Lung cancer cell. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/
description: Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions
Processing history: colorized
DOI: 10.6075/j05t3jv7
title: CIL:39791, Lytechinus pictus, mesenchymal cell, epithelial cell. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data
description: Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. This high magnification image of the embryo shows the primary mesenchyme syncytial ring, where it attaches to the outer epithelial layer (ectoderm layer). The fine fibers are part of the blastocoel matrix. The primary mesenchyme syncytial ring is the precursor to spicule formation (developing calcareous larval skeleton).This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.
description: Images taken on a JEOL 35C SEM. 
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW 
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.
DOI: 10.6075/j00001j1
title: CIL: 50889, Saccharomyces cerevisiae (baker's yeast, budding yeast), Mixed population of S. cerevisiae cells. In Cell Image Library

description: Heat shocked cells were cultured in YPD medium and high pressure frozen in a Wohlwend Compact 3 machine,
 followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. For the immuno-EM, samples were blocked for 2 hours in 0.8% BSA with 0.1% fish skin gelatine. A 1:10 dilution of the primary antibody ab19247 (Abcam) was used and a 1:20 dilution of the secondary antibody anti-Rabbit IgG 10 nm gold (Cat#25108, Electron microscopy sciences). Finally, samples were post fixed in 2.5% glutaraldehyde for 20 minutes. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead 
citrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera.
description: Imaging mode: transmission electron microscopy (TEM)
Visualization methods: Stain with Uranyl acetate and Lead citrate
description: Heat shocked Saccharomyces cerevisiae cells (38 oC for up to 90 minutes) were high pressure frozen and embedded in HM20 resin. Samples were then stained for Ubiquitin with the polyclonal antibody ab19247 (Abcam) and visualized through transmission electron microscopy (TEM). The images show the nucleus of the cells and the black arrow indicates nuclear envelope budding events. The black gold particles represent the presence of Ubiquitin.
DOI: 10.6075/j0pn950p
title: CIL:38863, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: The raw film was scanned with a Nikon Coolscan 9000ED. The digitized image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: High resolution image of Didinium (non-dividing) has a massive cytopharynx at its anterior end that takes the shape of a cone-shaped proboscis when closed. This cytopharynx contains numerous lamellae composed of sets of microtubules that lie perpendicular to each other next to the cytopharyngeal membrane. Vesicles containing a uniform medium dense material (mucocysts?), line up between the lamellae. Presumably these vesicles fuse with the single cytopharyngeal membrane that covers the lamellae. Extrusomes called toxicysts lie within in the cytopharyngeal region. TEM taken on 9/19/68 by R. Allen with Philips 300 operating at 60kV. Neg. 10,300X.
DOI: 10.6075/j0rf5t85
title: CIL:562, Acetabularia mediterranea, gamete. In Cell Image Library

description: At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium has just begun to divide into uninuclear gametes. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.
description: Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.
description: Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data
DOI: 10.6075/j0x929pc
title: CIL: 50843, Saccharomyces cerevisiae (baker's yeast, budding yeast), Mixed population of S. cerevisiae cells. In Cell Image Library

description: Imaging mode: transmission electron microscopy (TEM)
Visualization methods: Stain with Uranyl acetate and Lead citrate
description: Treated cells were cultured in YPD medium and high pressure frozen in a Wohlwend Compact 3 machine,
 followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead 
citrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera.
description: Wild type Saccharomyces cerevisiae cells (S228C background) were treated with 0.5 mM of sodium arsenite for 60 minutes. Then, samples were high pressure frozen and visualized through transmission electron microscopy (TEM). The images show the nucleus of the cells and the black arrow indicates nuclear envelope budding events.
DOI: 10.6075/j01g0k0m
title: CIL:13896, Saccharomyces cerevisiae. In Cell Image Library

description: Cells (MATa tem1::KanMX ura3::eGFP-CNM67–TEM1::URA3 kin4::TRP1-pGAL1-10-KIN4) were grown in rich medium containing 2% galactose/ 2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
DOI: 10.5281/zenodo.17492887
title: SOILCRATES Soil Health Dataset

description: Test version
DOI: 10.6075/j0j966dn
title: CIL:35687, Saccharomyces cerevisiae. In Cell Image Library

description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP taggedMAD2. 
 
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, mad2 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad2p forms a tight complex with another spindle checkpoint protein, Mad1p, throughout the cell cycle. Mad2p also forms a complex with Cdc20p, which activates the anaphase promoting complex, and Mad3p; the presence of Mad1p is required for the complex to form. Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and the protein kinase Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation. Homologs of Mad2p act in the spindle checkpoint in Xenopus (Xmad2) and human (MAD2L1 andMAD2L2.MAD2 was originally identified as the open reading frame YJL031C. However, it is now known thatMAD2 corresponds to the adjacent open reading frame YJL030W, while YJL031C encodes the geranylgeranyltransferase subunit Bet4p.
 
These phase,gfp images are part of an image group that ranges from CIL:35680-35699. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
description: Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
DOI: 10.5281/zenodo.17492886
title: SOILCRATES Soil Health Dataset

description: Test version
DOI: 10.5281/zenodo.14267261
title: The assessment of the use of e-health services in Polish society in the post-pandemic era: the results of a large-scale cross-sectional study

description: The dataset used in the analysis reported in the paper titled: "The assessment of the use of e-health services in Polish society in the post-pandemic era: the results of a large-scale cross-sectional study" submitted and accepted for the World Congres on Medical and Health Informatics 2025, Taipei, Taiwan, August 9-13, 2025. The paper is planned to be published in the post-conference book by IOS Press. 

This research was funded by the National Science Centre, Poland, within the project titled: "Determinants of acceptance and readiness to use of e-health applications and Health 4.0 technologies in Polish society" (grant number: 2021/43/B/NZ7/02140).
DOI: 10.5281/zenodo.14267260
title: The assessment of the use of e-health services in Polish society in the post-pandemic era: the results of a large-scale cross-sectional study

description: The dataset used in the analysis reported in the paper titled: "The assessment of the use of e-health services in Polish society in the post-pandemic era: the results of a large-scale cross-sectional study" submitted and accepted for the World Congres on Medical and Health Informatics 2025, Taipei, Taiwan, August 9-13, 2025. The paper is planned to be published in the post-conference book by IOS Press. 

This research was funded by the National Science Centre, Poland, within the project titled: "Determinants of acceptance and readiness to use of e-health applications and Health 4.0 technologies in Polish society" (grant number: 2021/43/B/NZ7/02140).
DOI: 10.6075/j0f47n9d
title: CIL:15713, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The contractile vacuole and cortex of Didinium nasutum showing an extrusive organelle (cyrtocyst) of unknown function. Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 90nm thickness.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements