DOI: 10.5061/dryad.8ht04
title: Data from: Phylogeography of the prickly sculpin (Cottus asper) in north-western North America reveals parallel phenotypic evolution across multiple coastal–inland colonizations

description: Aim: Glacial cycles during the Pleistocene may have frequently contributed
 to parallel evolution of phenotypes across independently evolving genetic
 lineages associated with separate glacial refugia. Previous studies based
 on morphology suggested that the prickly sculpin (Cottus asper) survived
 the Last Glacial Maximum (LGM) in southern coastal and inland refugia,
 favouring allopatric divergence between coastal and inland prickling
 phenotypes, which vary in the degree to which spine-like scales cover the
 body of the fish. Herein, we aimed to test whether parallel evolution
 across multiple genetic lineages rather than a single-lineage origin of
 highly prickled inland sculpins could serve as an explanation for the
 biogeographical distribution of prickling phenotypes. Location:
 North-western North America, Southeast Alaska and Canada (British
 Columbia). Methods: We used data from mitochondrial haplotypes and 19
 microsatellite loci to identify distinct genetic lineages as a basis to
 interpret patterns of phenotypic evolution. Results: The occurrence of
 multiple mtDNA groups suggests that highly prickled inland phenotypes
 comprise more than one genetic lineage. Both mtDNA and microsatellite data
 are consistent with post-glacial dispersal along the coast and repeated
 coastal to inland colonization events, as opposed to inland dispersal of a
 single lineage from a southern refugium to northern regions. Main
 conclusions: Our results suggest that highly prickled inland phenotypes
 evolved repeatedly following multiple inland colonization events, probably
 via coastal rivers. The prickly sculpin therefore provides an example of
 recent (post-glacial) parallel evolution, potentially facilitated by
 standing genetic variation already present in the ancestral coastal
 populations.
description: Cottus asper, Genepop
 file for 19 microsatellite lociGenepop datafile for 19
 microsatellite loci genotyped for Cottus asper. Sampling locations include
 Alaska (Auke Creek), British Columbia/Canada (Falls Creek, Little Campbell
 River, Meziadin Lake, McLeod Lake, Okanagan Lake, Peace River, Bella Coola
 River, Harrison Lake, Lakelse Lake, Martins Lake and outlet, Mosquito
 Lake, Nimpo Lake, Tlell River), and Alberta/Canada (Peace
 River).DRYAD_comb19MSA.genLandmark raw data for Cottus asper geometric morphometricsTPS landmark raw data file, including coordinates for 23 landmarks for 733 individuals. Image identifiers read as follows: CL = Cowichan Lake; HL = Harrison Lake; MEL = Meziadin Lake; ML = McLeod Lake; MOL = Mosquito Lake; OL = Okanagan Lake; streamBCRD = Bella Coola River; streamFC = Falls Creek; streamLCRD = Little Campbell River; streamPER = Peace River; streamWCD = Williams Creek; PAR = Parsnip River; PL = Paq Lake ; POIL = Poirier Lake; PROL = Prospect Lake; RL = Ruby Lake; SL = Skidegate Lake; TR = Tlell River; GL Graham Lake= ; KR = Keogh River; LAL = Lakelse Lake; LCRU = Little Campbell River Upstream; MAL = Martins Lake; MALI = Martins Lake Inlet; MALO = Martins Lake Outlet; MCL = McCreight Lake; NAR = Nass River; NIL = Nimpo Lake; NKL = Nimpkish Lake; NL = North Lake;CottusAsper_LandmarkRawData.tps