DOI: 10.5061/dryad.ch86c
title: Data from: Behavioral isolation due to cascade reinforcement in Lucania killifish

description: Reinforcement occurs when behavioral isolation is strengthened between
 species due to selection against hybridization in sympatry. Mate
 preferences and their target traits may change in sympatry as a
 consequence of reinforcement. This can potentially generate further
 behavioral isolation within species if sympatric populations evolve
 extreme preferences or traits that cause them to reject individuals from
 foreign populations as mates or be rejected as mates. This process is
 known as cascade reinforcement. We measured behavioral isolation between
 sympatric and allopatric populations of Lucania killifish to determine
 whether isolation evolves due to reinforcement between species and whether
 reinforcement affects preferences within species, consistent with the
 cascade reinforcement hypothesis. We measured mate preferences in both
 sexes between species (Lucania parva vs. Lucania goodei) and within
 species (among populations of L. parva). Between species, both male and
 female preferences for conspecifics were highest in sympatric populations.
 Within species, L. parva females from sympatric populations preferred
 their own native males over foreign males. Allopatric L. parva females and
 all L. parva males showed no preferences within species. Within species,
 behavioral isolation showed no association with ecological variables, such
 as salinity. Thus, reinforcement is a primary factor generating behavioral
 isolation in Lucania killifish, creating strong preferences in both sexes
 among species and leading to cascade reinforcement of female mate
 preference within species.
description: Between species mating
 trial data This file contains the
 data for time spent with conspecific (Con) or heterospecific (Het) mates
 when focal fish had the choice between both. Time measured in seconds (out
 of 600 second trials). Allopatric and Sympatric focal fish compared. Data
 for Figure 2 of the paper. claremalevf3Rev.txt
 
 Between
 population female mating trial data This file contains
 association time (in seconds) for female <em>L. parva</em>
 given the choice between males of their own population or males of a
 foreign population. Foreign populations could either be from the same
 salinity environment (ecology) or different salinity. Females came from
 populations that were either sympatric with <em>L. goodei</em>
 or allopatric. Data for Figure 3 and Figure 5. GRdata3Rev.txt
 
 Between
 population male mating trial data This file contains
 association time (in seconds) for <em>L. parva</em> males
 choosing between females from their own population or a foreign
 population. Focal males were from populations sympatric with <em>L.
 goodei</em> or allopatric. Data from Figure 4, Figure 5, and Figure
 A2. amymRed.txt
 
 Between
 population egg laying data 2 hours Number of eggs female
 <em>L. parva</em> laid with males from their own or foreign
 populations over a 2-hour period with each male. Data for Figure
 6. eggs.txt
 
 Between
 population egg laying data 10 days Number of eggs laid over
 10 days for sympatric Indian River (IR) females and allopatric Texas (TX)
 females in no-choice trials. Data for Figure A3. IRTX_premate2.txt
 
 Male
 morphology Standard length and
 total anal fin area as measured from pictures of <em>L.
 parva</em> (IR,SJ,GC,LB) and <em>L. goodei </em>(UB)
 males. Data for Figure A4. parvamales2.txt
 
 Latency to mate
 between species Latency to mate (in
 days) when Indian River <em>L. parva</em> (IR-sympatric) were
 paired with Blue Springs <em>L. goodei </em>(BS-allopatric).
 Data for Figure A5. BSIRlatency.txt