cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.rh7ges
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Aplocheilichthys lacustris, Lacustricola lacustris, Aplocheilichthys macrurus, Lacustricola macrurus, Aplocheilichthys maculatus, Lacustricola maculatus, Aplocheilichthys mediolateralis, Lacustricola mediolateralis, Aplocheilichthys nigrolateralis, Lacustricola nigrolateralis, Aplocheilichthys omoculatus, Lacustricola omoculatus, Aplocheilichthys rudolfianus, Micropanchax rudolfianus, Aplocheilichthys spilauchen, Aplocheilichthys usanguensis, Lacustricola usanguensis, Aplocheilichthys vitschumbaensis, Lacustricola vitschumbaensis, Aplocheilus panchax, Apricaphanius saourensis, Archiaphyosemion guineense, Aristochromis christyi, Arius africanus, Arius festinus, Arius gigas, Arius latiscutatus, Arius madagascariensis, Arius uncinatus, Arnoglossus imperialis, Arnoldichthys spilopterus, Asprotilapia leptura, Xenotilapia leptura, Astatoreochromis alluaudi, Astatoreochromis straeleni, Astatoreochromis vanderhorsti, Astatotilapia bloyeti, Astatotilapia burtoni, Astatotilapia calliptera, Astatotilapia desfontainii, Astatotilapia paludinosa, Haplochromis paludinosus, Astatotilapia stappersii, Astatotilapia tweddlei, Atherina boyeri, Atherina caspia, Atherinomorus duodecimalis, Atopochilus chabanaudi, Atopochilus christyi, Atopochilus macrocephalus, Atopochilus mandevillei, Atopochilus pachychilus, Atopochilus savorgnani, Atopochilus vogti, Atopodontus adriaensi, Auchenoglanis biscutatus, Auchenoglanis occidentalis, Aulonocara aquilonium, Aulonocara auditor, Aulonocara baenschi, Aulonocara brevinidus, Aulonocara brevirostre, Aulonocara ethelwynnae, Aulonocara gertrudae, Aulonocara guentheri, Aulonocara hueseri, Aulonocara jacobfreibergi, Aulonocara kandeense, Aulonocara kandeensis, Aulonocara koningsi, Aulonocara korneliae, Aulonocara maylandi, Aulonocara nyassae, Aulonocara rostratum, Aulonocara saulosi, Aulonocara stonemani, Aulonocara stuartgranti, Aulonocara trematocephalum, Aulonocranus dewindti, Aurigequula fasciata, Auriglobus modestus, Austroglanis barnardi, Austroglanis gilli, Austroglanis sclateri, Awaous aeneofuscus, Awaous bustamantei, Awaous commersoni, Awaous lateristriga, Awaous macrorhynchus, Bagrus bajad, Bagrus caeruleus, Bagrus degeni, Bagrus docmak, Bagrus filamentosus, Bagrus lubosicus, Bagrus meridionalis, Bagrus orientalis, Bagrus ubangensis, Bagrus urostigma)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((10.1382 29.0599,1.1382 29.0599,1.1382 20.0599,10.1382 20.0599,10.1382 29.0599))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005783-250827131500795/datasets/export for details.
DOI: 10.22002/edhj9-n0y22
title: Myxococcus xanthus MT299

description: BacM-mCherry correlated cryofLM/ECT imaging
description: <strong>Tilt Series Date:</strong> 2013-08-19</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu Finder</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-1/rawdata/</p>20130819_MT299_cryoLM_10001.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-1/rawdata/20130819_MT299_cryoLM_10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130819_MT299_cryoLM_10001_part131_7.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-1/3dimage_29765/20130819_MT299_cryoLM_10001_part131_7.rec"
        > <i class="download icon"></i> Download </a></p>

        20130819_MT299_cryoLM_10001_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 267.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-19-1/3dimage_30220/20130819_MT299_cryoLM_10001_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hb6s3-6a005
title: Myxococcus xanthus PilO-sfGFP

description: Grid box YWC110Strain SA4088
description: <strong>Tilt Series Date:</strong> 2014-07-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 31 hr in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.6</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-26/rawdata/</p>20140716_YWC110_10028.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-26/rawdata/20140716_YWC110_10028.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140716_YWC110_10028.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-26/3dimage_44835/20140716_YWC110_10028.rec"
        > <i class="download icon"></i> Download </a></p>

        20140716_YWC110_10028_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-26/3dimage_44957/20140716_YWC110_10028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/q2ysn-30r92
title: MS411-R1-16 / WM1652

description: to capture F-piligrid box: YWC095
description: <strong>Tilt Series Date:</strong> 2014-04-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strains from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MS411-R1-16 & WM1652 grew to OD 0.6 in LB. Mix 2.5 ul of each onto grids on LB-agar plate, incubate 30 min for conjugation.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 22.5 C, 3µl BSA treated 10 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 1 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-7/rawdata/</p>20140407_YWC095_20006.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-7/rawdata/20140407_YWC095_20006.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140407_YWC095_20006.rec,  Reconstruction (Pixel Size 1.92 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-7/3dimage_41133/20140407_YWC095_20006.rec"
        > <i class="download icon"></i> Download </a></p>

        20140407_YWC095_20006_DB.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-07-7/3dimage_41299/20140407_YWC095_20006_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/j678a-xrp64
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-23/rawdata/</p>20141022_YWC121_30010.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-23/rawdata/20141022_YWC121_30010.mrc"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_30010.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-23/3dimage_47406/20141022_YWC121_30010.rec"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_30010_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-23/3dimage_47471/20141022_YWC121_30010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4hs7k-2cf16
title: Coxiella_ Verocells_28dpi

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells.
description: <strong>Tilt Series Date:</strong> 2017-10-19</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab.</p>
<strong>Sample Preparation:</strong> Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-9/rawdata/</p>Cb_vero_201710190007.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-9/rawdata/Cb_vero_201710190007.mrc"
        > <i class="download icon"></i> Download </a></p>

        Cb_vero_201710190007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-19-9/3dimage_89450/Cb_vero_201710190007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-10-19-9_slicer19070.png,  REZ / nucleoid with flow lines .  pixel size 1.56nm .  dividing cell .  crazy membranes .  -andrew, 482.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-10-19-9_slicer19070.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.f58rfa
title: Occurrence Download

description: A dataset containing 349803 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Cairina moschata",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 349803 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005764-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/epe1c-0bz81
title: LCV_Legionella pneumophila

description: THP1 cells were infected with Legionella wild type cells and thereafter phagosomes (LCV) were purified by sucrose density gradient method. Phagoseomes were fixed before shipment.
description: <strong>Tilt Series Date:</strong> 2017-04-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Phagosome isolation method as described by Joelle Gabay (Endnote).</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-22/rawdata/</p>LCV_wt_20170412d0017.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-22/rawdata/LCV_wt_20170412d0017.mrc"
        > <i class="download icon"></i> Download </a></p>

        LCV_wt_20170412d0017_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-22/3dimage_83618/LCV_wt_20170412d0017_full.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-04-14-22_slicer18033.png, , 874.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-04-14-22_slicer18033.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0r54t-qk324
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-120/rawdata/</p>atlas_20018.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-120/rawdata/atlas_20018.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_20018_part51_7.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-120/3dimage_89164/atlas_20018_part51_7.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_20018_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-120/3dimage_90302/atlas_20018_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9j4cw-x9967
title: S. acidocaldarius

description: Raw data files of S. acidocaldarius
description: <strong>Tilt Series Date:</strong> 2017-11-22</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Megan's cell stock</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells in ATCC 1723 medium at 76 C to late log or stationary phase, constantly shaking at >220 rpm and topping up H2O that evaporates (every 12-16 hrs).</p>
<strong>Sample Preparation:</strong> Resuspend cells to ~5-10 OD in the same medium. Freeze 3.5 µL of cells in Cu finder grids by blotting manually from behind. A very aggressive blotting technique leads to dry cells. Some frozen grids were FIB milled after Pt coating (2 mA for 10 sec) and some frozen grids were coated with 3-5 nm Au beads in 2-methyl butane (washed later with Ethane/Propane)</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-11-22-16/rawdata/</p>TS8.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-11-22-16/rawdata/TS8.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS8_4bin_SIRTrotX_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-11-22-16/3dimage_91655/TS8_4bin_SIRTrotX_DB.rec"
        > <i class="download icon"></i> Download </a></p>

        TS8_4bin_WBP_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-11-22-16/3dimage_91656/TS8_4bin_WBP_DB.rec"
        > <i class="download icon"></i> Download </a></p>