DOI: 10.5061/dryad.71425
title: Data from: Multiple pairwise analysis of non-homologous centromere coupling reveals preferential chromosome size-dependent interactions and a role for bouquet formation in establishing the interaction pattern

description: During meiosis, chromosomes undergo a homology search in order to locate
 their homolog to form stable pairs and exchange genetic material. Early in
 prophase, chromosomes associate in mostly non-homologous pairs, tethered
 only at their centromeres. This phenomenon, conserved through higher
 eukaryotes, is termed centromere coupling in budding yeast. Both
 initiation of recombination and the presence of homologs are dispensable
 for centromere coupling (occurring in spo11 mutants and haploids induced
 to undergo meiosis) but the presence of the synaptonemal complex (SC)
 protein Zip1 is required. The nature and mechanism of coupling have yet to
 be elucidated. Here we present the first pairwise analysis of centromere
 coupling in an effort to uncover underlying rules that may exist within
 these non-homologous interactions. We designed a novel chromosome
 conformation capture (3C)-based assay to detect all possible interactions
 between non-homologous yeast centromeres during early meiosis. Using this
 variant of 3C-qPCR, we found a size-dependent interaction pattern, in
 which chromosomes assort preferentially with chromosomes of similar sizes,
 in haploid and diploid spo11 cells, but not in a coupling-defective mutant
 (spo11 zip1 haploid and diploid yeast). This pattern is also observed in
 wild-type diploids early in meiosis but disappears as meiosis progresses
 and homologous chromosomes pair. We found no evidence to support the
 notion that ancestral centromere homology plays a role in pattern
 establishment in S. cerevisiae post-genome duplication. Moreover, we found
 a role for the meiotic bouquet in establishing the size dependence of
 centromere coupling, as abolishing bouquet (using the bouquet-defective
 spo11 ndj1 mutant) reduces it. Coupling in spo11 ndj1 rather follows
 telomere clustering preferences. We propose that a chromosome size
 preference for centromere coupling helps establish efficient homolog
 recognition.
description: DipNdj_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for spo11 ndj1 diploidsDipNdj_EnrichmentFinal.xlsxDipRec_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for spo11 rec8 diploidsDipRec_EnrichmentFinal.xlsxDipSpo_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for spo11 diploidsDipSpo_EnrichmentFinal.xlsxDipZip_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for spo11 zip1 diploidsDipZip_EnrichmentFinal.xlsxHapSpo_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for spo11 haploidsHapSpo_EnrichmentFinal.xlsxHapZip_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for spo11 zip1 haploidsHapZip_EnrichmentFinal.xlsxWT8h_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for WT yeast, time point 8h (early)WT8h_EnrichmentFinal.xlsxWT9h_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for WT yeast, time point 9h (early)WT9h_EnrichmentFinal.xlsxWT10h_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for WT yeast, time point 10h (mid)WT10h_EnrichmentFinal.xlsxWT11h_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for WT yeast, time point 11h (mid)WT11h_EnrichmentFinal.xlsxWT14h_FinalMean Cp values and Normalized Enrichment Ratio (Interaction frequencies) + standard deviation for WT yeast, time point 14h (late)WT14h_EnrichmentFinal.xlsx