cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11997771
title: Critical nitrogen dilution curves and productivity assessments for plant cane

description: ABSTRACT Nitrogen is the most limiting nutrient for the development of sugarcane and particularly the use of more productive and demanding varieties, for which the doses of nitrogen fertilization necessary during the plant cane cycle may be underestimated and the critical levels of nitrogen in the leaf may be modified. The objective of this study was to determine the critical nitrogen dilution curve (CNDC) for leaves, evaluate the aboveground dry mass production (DM) and productivity of two varieties fertilized with N in the plant cane cycle. The study was conducted under field conditions, evaluating two sugarcane varieties RB92579 and RB867515, fertilized with seven doses of N (0, 30, 60, 90, 120, 150 and 200 kg ha-1). The varieties and doses were arranged in randomized blocks and analyzed in the 2 × 7 factorial scheme. It was found that the nitrogen concentration in the leaf decreased with four repetitions increase in DM yield and adjusted an exponential model, which enabled to estimate the critical levels of 13.03 and 12.46 g N kg-1 dry matter in the varieties RB92579 and RB867515, respectively. The plant cane cycle were observed to respond positively to nitrogen fertilization, with increase of 18.66% in tons of stalk per hectare (TSH) in response to the application addition of up to 129.45 kg N ha-1. The variety RB92579 showed the lowest dilution of the critical nitrogen concentration, the highest production of DM and higher stalk yield, and accordingly is considered to be the more efficient of the two varieties with respect to nitrogen fertilizer utilization.
DOI: 10.6084/m9.figshare.11997777
title: Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats

description: Abstract The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the Brazilian Microbiome Project pipeline for Ion Torrent data with modifications. Statistical analyses were performed in the R software environment. We found that chronic feeding of a commercial NNS-sweetened yogurt to Wistar rats, within the recommended dose range, did not significantly modify gut microbiota composition in comparison to sucrose-sweetened yogurt. Our findings do not support the hypothesis that moderate exposure to NNS is associated with changes in gut microbiota pattern compared to sucrose, at least in this experimental model.
DOI: 10.6084/m9.figshare.11997777.v1
title: Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats

description: Abstract The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the Brazilian Microbiome Project pipeline for Ion Torrent data with modifications. Statistical analyses were performed in the R software environment. We found that chronic feeding of a commercial NNS-sweetened yogurt to Wistar rats, within the recommended dose range, did not significantly modify gut microbiota composition in comparison to sucrose-sweetened yogurt. Our findings do not support the hypothesis that moderate exposure to NNS is associated with changes in gut microbiota pattern compared to sucrose, at least in this experimental model.
DOI: 10.6084/m9.figshare.11997771.v1
title: Critical nitrogen dilution curves and productivity assessments for plant cane

description: ABSTRACT Nitrogen is the most limiting nutrient for the development of sugarcane and particularly the use of more productive and demanding varieties, for which the doses of nitrogen fertilization necessary during the plant cane cycle may be underestimated and the critical levels of nitrogen in the leaf may be modified. The objective of this study was to determine the critical nitrogen dilution curve (CNDC) for leaves, evaluate the aboveground dry mass production (DM) and productivity of two varieties fertilized with N in the plant cane cycle. The study was conducted under field conditions, evaluating two sugarcane varieties RB92579 and RB867515, fertilized with seven doses of N (0, 30, 60, 90, 120, 150 and 200 kg ha-1). The varieties and doses were arranged in randomized blocks and analyzed in the 2 × 7 factorial scheme. It was found that the nitrogen concentration in the leaf decreased with four repetitions increase in DM yield and adjusted an exponential model, which enabled to estimate the critical levels of 13.03 and 12.46 g N kg-1 dry matter in the varieties RB92579 and RB867515, respectively. The plant cane cycle were observed to respond positively to nitrogen fertilization, with increase of 18.66% in tons of stalk per hectare (TSH) in response to the application addition of up to 129.45 kg N ha-1. The variety RB92579 showed the lowest dilution of the critical nitrogen concentration, the highest production of DM and higher stalk yield, and accordingly is considered to be the more efficient of the two varieties with respect to nitrogen fertilizer utilization.
DOI: 10.6084/m9.figshare.11997747
title: Environmental recovery dynamics of waste dumps in calcary mining by natural regeneration

description: ABSTRACT The present research had as objective to evaluate the dynamics of the environmental recovery of waste dumps in calcary mining by analyzing the composition and phytosociological structure of natural regeneration. The study was carried out in a waste dumo located in Salto de Pirapora, São Paulo, Brazil, with sterile piles abandoned since 1994 and 2013 and a native forest fragment, totaling three treatments. Twelve plots of 10 × 10 m were allocated to each area, where all shrub-tree individuals with height above 1.30 m were sampled, identified and evaluated for their composition and phytosociological structure. The occurrence of natural regeneration in the sterile cells was observed with the increased number of individuals and species over time. The results indicate that the vegetation present in the waste dumps is in transition from pioneer forest to a more advanced successional stage. However, Leucaena leucocephala has established itself more expressively than other species, inhibiting the development of other species. Finally, management actions are suggested so that the ecological succession of the waste dumps is not compromised.
DOI: 10.6084/m9.figshare.11997753
title: Chemical composition of Panicum maximum ‘BRS Zuri’ subjected to levels of salinity and irrigation depths

description: ABSTRACT The study of cultivars with moderate flood tolerance is essential to their use in pastures irrigated with saline water. The aim of this study was to evaluate the chemical composition of Panicum maximum ‘BRS Zuri’ under the effect of different irrigation depths and levels of salinity. A randomised block design of split plots was used with five replications per treatment. The treatments resulted from the combination of three levels of water salinity (S1 = 0.6, S2 = 1.8 and S3 = 3.0 dS m-1) and four levels of irrigation (I1 = 60%, I2 = 80%, I3 = 100% and I4 = 120% of the evapotranspiration). The chemical composition of the grass, Panicum maximum ‘BRS Zuri’, cut at 28 days, was evaluated over two cycles. There was an interaction between the factors (salinity x irrigation depth x cycle) for the dry matter (DM) content. With the increase in irrigation depth, there was a linear reduction in the crude protein (CP) content in both cycles. The neutral detergent fibre (NDF) and cellulose (CEL) content increased linearly as a function of the irrigation depth, while the lignin (LIG) content decreased linearly with irrigation depth. It was concluded that high levels of salinity cause reductions in the dry matter content in response to increased water availability and extension of the cultivar cycles. The reduction in saline levels under low water availability results in a higher CP content. Salinity has a negative effect on the NDF, ADF, HEM, CEL and LIG content as the irrigation depth increases.
DOI: 10.6084/m9.figshare.11997747.v1
title: Environmental recovery dynamics of waste dumps in calcary mining by natural regeneration

description: ABSTRACT The present research had as objective to evaluate the dynamics of the environmental recovery of waste dumps in calcary mining by analyzing the composition and phytosociological structure of natural regeneration. The study was carried out in a waste dumo located in Salto de Pirapora, São Paulo, Brazil, with sterile piles abandoned since 1994 and 2013 and a native forest fragment, totaling three treatments. Twelve plots of 10 × 10 m were allocated to each area, where all shrub-tree individuals with height above 1.30 m were sampled, identified and evaluated for their composition and phytosociological structure. The occurrence of natural regeneration in the sterile cells was observed with the increased number of individuals and species over time. The results indicate that the vegetation present in the waste dumps is in transition from pioneer forest to a more advanced successional stage. However, Leucaena leucocephala has established itself more expressively than other species, inhibiting the development of other species. Finally, management actions are suggested so that the ecological succession of the waste dumps is not compromised.
DOI: 10.6084/m9.figshare.11997753.v1
title: Chemical composition of Panicum maximum ‘BRS Zuri’ subjected to levels of salinity and irrigation depths

description: ABSTRACT The study of cultivars with moderate flood tolerance is essential to their use in pastures irrigated with saline water. The aim of this study was to evaluate the chemical composition of Panicum maximum ‘BRS Zuri’ under the effect of different irrigation depths and levels of salinity. A randomised block design of split plots was used with five replications per treatment. The treatments resulted from the combination of three levels of water salinity (S1 = 0.6, S2 = 1.8 and S3 = 3.0 dS m-1) and four levels of irrigation (I1 = 60%, I2 = 80%, I3 = 100% and I4 = 120% of the evapotranspiration). The chemical composition of the grass, Panicum maximum ‘BRS Zuri’, cut at 28 days, was evaluated over two cycles. There was an interaction between the factors (salinity x irrigation depth x cycle) for the dry matter (DM) content. With the increase in irrigation depth, there was a linear reduction in the crude protein (CP) content in both cycles. The neutral detergent fibre (NDF) and cellulose (CEL) content increased linearly as a function of the irrigation depth, while the lignin (LIG) content decreased linearly with irrigation depth. It was concluded that high levels of salinity cause reductions in the dry matter content in response to increased water availability and extension of the cultivar cycles. The reduction in saline levels under low water availability results in a higher CP content. Salinity has a negative effect on the NDF, ADF, HEM, CEL and LIG content as the irrigation depth increases.
DOI: 10.6084/m9.figshare.11997723
title: Passiflora mucronata leaves extracts obtained from different methodologies: a phytochemical study based on cytotoxic and apoptosis activities of triterpenes and phytosterols constituents

description: Cancer is one of the most prevalent diseases worldwide and the natural products could be a source of bioactive compounds. Passiflora mucronata (PM) belongs to a very known vegetal genus, although, there are no studies about cytotoxic activity or isolated compounds. Different extracts from PM were obtained by liquid-liquid partition (P), Soxhlet (Sox) and supercritical fluid (SFE1-5) extraction techniques, being compared concerning their yields, chemical profile and cytotoxicity. The Sox extracts showed the highest yields (6.03%: hexane; 2.51%: dichloromethane) followed by SFE (from 4.34 to 1.63%) and partitions (1.06 and 2.26%). The hexane partition (HP) showed the best cytotoxic activity against K562 cell line (IC50 = 18.72 µg.mL-1). From HP, the following compounds were identified and analysed its cytotoxic activities: β-amyrin (IC50 = 3.92 µg.mL-1), β-sitosterol (IC50 = 3.37 µg.mL-1), stigmasterol (IC50 = 3.31 µg.mL-1) and oleanolic acid. Stigmasterol induced about 75% of K562 total apoptosis. The compounds were tested against MA-104 cell line and the selective index (SI) attributed (SI &gt;10 for all compounds). This indicates good selectivity to K562 cell line at the expense of MA-104. This is the first time, identifying those compounds to PM .
DOI: 10.6084/m9.figshare.11997720
title: Assessment tool for Integrated Solid Waste Management Municipal Plans: part 1

description: ABSTRACT Proper management of solid waste is a challenge for Brazilian municipalities. Considering the requirements of the National Solid Waste Policy (Política Nacional de Resíduos Sólidos - PNRS), an important step in this adaptation is the elaboration of the Municipal Plan of Integrated Solid Waste Management (Plano Municipal de Gestão Integrada de Resíduos Sólidos - PMGIRS). Considering the importance of this planning instrument, this article proposes a tool for evaluating these plans. The methodological path of construction and validation was configured in three stages: conception of the indicators from bibliographic and documentary review; validation with experts, in which indicators were validated and weighting factors were defined using the Delphi technique; and application validation, which consisted of applying the tool to evaluate the PMGIRS of six Brazilian municipalities of different sizes. This article will present the results of the first and second stages. The results of the third stage will be presented in part 2 of this article. The tool allows to verify the fulfillment of PNRS requirements, the quality of the plans, the complexity of its elaboration, and its potential of implementation and continuity of the established actions. With this, it is expected to minimize the gap related to the monitoring of the various stages of PNRS implementation. .
DOI: 10.6084/m9.figshare.11997714
title: Esculetin as bioactive marker: towards a rational scientific approach for the treatment of hyperuricemia using Traditional Chinese Medicine

description: Traditional Chinese Herbal Medicine has been used to prevent and cure disease in China for thousands of years and has gained global interest in recent decades. The Erding formula is a Chinese Pharmacopeia (ChP)-listed herbal preparation used for treating sore throat, carbuncles and boils. Esculetin is a ChP quality control (QC) marker for these indications. A previous study found that a new indication, hyperuricemia, can be added to the Erding formula. Therefore, this study aimed to evaluate whether the traditionally used marker, esculetin, still has bioactivity for hyperuricemia, which is substantially different from the original indications. The study analyzed the quantity of esculetin by high-performance liquid chromatography, assessed the therapeutic effect of esculetin using animal model, and then characterized esculetin and its metabolites in serum via ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The results showed that the esculetin content in the aqueous Erding extract was 0.26±0.05% (w/w). Both the Erding extract and esculetin significantly reduced uric acid levels. Six metabolites of esculetin were identified in mice serum. This study revealed a rational scientific approach to prove esculetin is a reliable bioactive and QC marker for Erding formula in hyperuricemia treatment which contributed to ensure product quality and therapeutic efficacy.
DOI: 10.6084/m9.figshare.11997720.v1
title: Assessment tool for Integrated Solid Waste Management Municipal Plans: part 1

description: ABSTRACT Proper management of solid waste is a challenge for Brazilian municipalities. Considering the requirements of the National Solid Waste Policy (Política Nacional de Resíduos Sólidos - PNRS), an important step in this adaptation is the elaboration of the Municipal Plan of Integrated Solid Waste Management (Plano Municipal de Gestão Integrada de Resíduos Sólidos - PMGIRS). Considering the importance of this planning instrument, this article proposes a tool for evaluating these plans. The methodological path of construction and validation was configured in three stages: conception of the indicators from bibliographic and documentary review; validation with experts, in which indicators were validated and weighting factors were defined using the Delphi technique; and application validation, which consisted of applying the tool to evaluate the PMGIRS of six Brazilian municipalities of different sizes. This article will present the results of the first and second stages. The results of the third stage will be presented in part 2 of this article. The tool allows to verify the fulfillment of PNRS requirements, the quality of the plans, the complexity of its elaboration, and its potential of implementation and continuity of the established actions. With this, it is expected to minimize the gap related to the monitoring of the various stages of PNRS implementation. .
DOI: 10.6084/m9.figshare.11997723.v1
title: Passiflora mucronata leaves extracts obtained from different methodologies: a phytochemical study based on cytotoxic and apoptosis activities of triterpenes and phytosterols constituents

description: Cancer is one of the most prevalent diseases worldwide and the natural products could be a source of bioactive compounds. Passiflora mucronata (PM) belongs to a very known vegetal genus, although, there are no studies about cytotoxic activity or isolated compounds. Different extracts from PM were obtained by liquid-liquid partition (P), Soxhlet (Sox) and supercritical fluid (SFE1-5) extraction techniques, being compared concerning their yields, chemical profile and cytotoxicity. The Sox extracts showed the highest yields (6.03%: hexane; 2.51%: dichloromethane) followed by SFE (from 4.34 to 1.63%) and partitions (1.06 and 2.26%). The hexane partition (HP) showed the best cytotoxic activity against K562 cell line (IC50 = 18.72 µg.mL-1). From HP, the following compounds were identified and analysed its cytotoxic activities: β-amyrin (IC50 = 3.92 µg.mL-1), β-sitosterol (IC50 = 3.37 µg.mL-1), stigmasterol (IC50 = 3.31 µg.mL-1) and oleanolic acid. Stigmasterol induced about 75% of K562 total apoptosis. The compounds were tested against MA-104 cell line and the selective index (SI) attributed (SI &gt;10 for all compounds). This indicates good selectivity to K562 cell line at the expense of MA-104. This is the first time, identifying those compounds to PM .
DOI: 10.6084/m9.figshare.11997714.v1
title: Esculetin as bioactive marker: towards a rational scientific approach for the treatment of hyperuricemia using Traditional Chinese Medicine

description: Traditional Chinese Herbal Medicine has been used to prevent and cure disease in China for thousands of years and has gained global interest in recent decades. The Erding formula is a Chinese Pharmacopeia (ChP)-listed herbal preparation used for treating sore throat, carbuncles and boils. Esculetin is a ChP quality control (QC) marker for these indications. A previous study found that a new indication, hyperuricemia, can be added to the Erding formula. Therefore, this study aimed to evaluate whether the traditionally used marker, esculetin, still has bioactivity for hyperuricemia, which is substantially different from the original indications. The study analyzed the quantity of esculetin by high-performance liquid chromatography, assessed the therapeutic effect of esculetin using animal model, and then characterized esculetin and its metabolites in serum via ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The results showed that the esculetin content in the aqueous Erding extract was 0.26±0.05% (w/w). Both the Erding extract and esculetin significantly reduced uric acid levels. Six metabolites of esculetin were identified in mice serum. This study revealed a rational scientific approach to prove esculetin is a reliable bioactive and QC marker for Erding formula in hyperuricemia treatment which contributed to ensure product quality and therapeutic efficacy.
DOI: 10.6084/m9.figshare.11997708
title: Inverse Design of Tapers by Bio-Inspired Algorithms

description: Abstract The efficiency of optical power transferred between two bidimensional waveguides, continuous waveguide and periodic subwavelength waveguide has been designed and optimized by using a taper composed by variable length segments (0.03 − 0.27 µm). Waveguides with variable width (270 − 330 nm) have been considered and the number of segments on the taper region has been varied from 10 to 15. The optimized taper was efficiently designed using bio-inspired algorithms based on the genetic algorithms and the artificial immune system. The power coupled has been computed using the frequency domain finite element method. The best solution is 20% shorter than previously existent tapers with coupling light efficiency above 90% over a broadband interval of frequencies and it exhibits high fabrication error tolerances.
DOI: 10.6084/m9.figshare.11997705
title: INVESTIGATING ONLINE RESPONSE STRATEGIES FOR ADDRESSING NEGATIVE WORD OF MOUTH

description: ABSTRACT The digital age has transformed how brands communicate and interact with their customers. One consequence is that the effect of negative word of mouth on a brand’s reputation has intensified. This study investigates various response strategies employed to protect organizations’ reputations in the online environment. Accordingly, it collected data through two methods. First, 10 semi-structured interviews were conducted with brand managers to identify the strategies that they used to minimize negative word of mouth in social media. Second, the social media interventions of different brands under management by agencies were collected to complement the interviews, and determine whether any additional strategies could be identified. The results showed that, when negative word of mouth events occurred, companies preferred to either apologize, hide the original message, respond in private rather than in public, or simply ignore the negative comments from customers.