DOI: 10.5061/dryad.mf4h560
title: Data from: Matriline effects on metamorphic traits in a natural system in the European common frog (Rana temporaria)

description: Successful reproduction is an important determinant of the fitness of an
 individual and of the dynamics of populations. Offspring of the European
 common frog (Rana temporaria) exhibit a high degree of variability in
 metamorphic traits. However, environmental factors alone cannot explain
 this phenotypic variability, and the influence of genetic factors remains
 to be determined. Here, we tested whether the maternal genotype influences
 developmental time, body size, and body condition of offspring in a forest
 pond in Germany. We collected fertilized eggs from all 57 clutches
 deposited in the pond. We used multilocus genotypes based on seven
 microsatellite loci to assign metamorphosed offspring to mothers and to
 determine the number of fathers for a single matriline. We tested the
 influence of genetic effects in the same environment by comparing
 variability of metamorphic traits within and between full‐sib offspring
 grouped to matrilines and tested whether multiple paternity increases the
 variability of metamorphic traits in a single matriline. The variability
 in size and body condition was higher within matrilines than between them,
 which indicates that these traits are more strongly influenced by
 environmental effects, which are counteracting underlying genetic effects.
 The developmental time varied considerably between matrilines and
 variability increased with the effective number of fathers, suggesting an
 additive genetic effect of multiple paternity. Our results show that
 metamorphic traits are shaped by environmental as well as genetic effects.
description: foglets_daysContains data on numbers of metamorphs leaving the pond per day (days are defined from median date of clutch deposition 2013/04/11 untill day of metamorphosis) and date.all_froglets_111017Contains data on date the metamorph was sampled, metamorph ID, body size (SVL in mm), Mass in g, day of emergence from the pond, day of migration (starts with the first metamoph collected). Total number of sampled metamorphs is 1943.meta_traits_clusterContains metamorph ID, probability of sibship calculated by Colony software, father ID, mother ID, cluster number, size of the metamorph (SVL in mm), Mass in g, day of metamorphosis (defined as days from 2013/04/11 onwards), day of migration (starts with the first metamophs found on 2013/07/08), date, fathers (same or all) and Matriline number. Total number of metamorphs is 733 (same father= 294, all fathers= 439).temp_rain_air_tempContains environmental variables. The date of measurement, daily mean water temperature in the pond (av_temp), daily standard deviation of mean water temperature in the pond (sd_temp, measurement of temperature every 3 hours), daily amount of rain in mm and mean air temperature in 2m above ground.froglets_genetics_NAContains microsatellite data on the 7 loci used in the study. Given are metamorph ID and allel per locus. Total number of genotyped metamorphs is 707.COLONY_FilesThe zip folder contains text files with microsatellite data. The microsatellite data from the metamorphs (genetic_data_froglets_cleaned.txt), the microsatellites for four eggs per clutch (genetic_data_LB_froglets.txt) used as maternal sibs, the marker error rates to be used in Colony (MarkerTypeErrorRate.txt) and the known maternal relationship and offspring genotypes as output from Colony.Code_analysis_figures_Dittrich_etal_2018_Ecology_EvolutionContains R-source code to conduct all analysis descriped in the main manuscript and to reproduce the figures.Code_supplement_Dittrich_etal_2018_Ecology_EvolutionContains R-source code to conduct all analysis descriped in the supplement and to reproduce the figures.