DOI: 10.5061/dryad.4xgxd256f
title: Data from: Nonlinearities between inhibition and T-type calcium channel activity bidirectionally regulate thalamic oscillations

description: Absence seizures result from 3-5 Hz generalized thalamocortical
 oscillations that depend on highly regulated inhibitory neurotransmission
 in the thalamus. Efficient reuptake of the inhibitory neurotransmitter
 GABA is essential, and reuptake failure worsens seizures. Here, we show
 that blocking GABA transporters (GATs) in acute brain slices containing
 key parts of the thalamocortical seizure network modulates epileptiform
 activity. As expected, we found that blocking either GAT1 or GAT3
 prolonged oscillations. However, blocking both GATs unexpectedly
 suppressed oscillations. Integrating experimental observations into
 single-neuron and network-level computational models shows how a
 non-linear dependence of T-type calcium channel opening on GABAB receptor
 activity regulates network oscillations. Receptor activity that is either
 too brief or too protracted fails to sufficiently open T-type channels
 necessary for sustaining oscillations. Only within a narrow range does
 prolonging GABAB receptor activity promote channel opening and intensify
 oscillations. These results have implications for therapeutics that
 modulate GABA transporters.
description: See the first two subsections of the Methods section in the
 associated paper. Oscillation recordings in acute
 thalamic slices and dynamic clamp recordings in thalamocortical neurons
 are included. Analysis and simulation results can be reproduced from code
 available here: https://github.com/luadam4c/m3ha_published.
description: Oscillations data:<br> <br> The data are separated
 into 4 directories, corresponding to the following perfusion
 conditions:<br> (1) control: bicuculline-ACSF<br> (2) no711:
 bicuculline-ACSF plus 4 μM NO-711 to block GAT1 transport<br> (3)
 snap5114: bicuculline-ACSF plus 100 μM SNAP-5114 to block GAT3
 transport <br> (4) dual: bicuculline-ACSF plus a combination of 4 μM
 NO-711 and 100 μM SNAP-5114 to simultaneously block both GAT1 and
 GAT3<br> <br> Raw multiunit data (filtered between 100-3000
 Hz) are packaged into MATLAB .mat files, one for each slice. Metadata and
 analyzed results for all 60 second sweeps are available as comma-separated
 value (.csv) files, one for each slice. Master scripts used for analysis
 were m3ha_oscillations_analyze.m, parse_all_multiunit.m, parse_multiunit.m, plot_measures.m.<br> <br> Dynamic clamp data: Raw dynamic clamp recording data are available as Axon Binary Format (.abf) files. Each trace includes a short current pulse response (current pulse applied at approximately 100-110 ms) and an IPSC response (IPSC applied at 1000 ms). To extract and reorganize the .abf files into individual traces using MATLAB, please use the master script m3ha_parse_dclamp_data.m with resavedataflag set to 1. Metadata and analyzed results for all traces are available as both a csv file (dclampdatalog_take4.csv) and a MATLAB .mat file (dclampdatalog_take4.mat).<br> <br> Master scripts used for analysis were m3ha_parse_dclamp_data.m, m3ha_plot_figure02.m, m3ha_plot_figure04.m.