DOI: 10.22002/zbs53-7ek47
title: NR1769pKM101conjFM433

description: skinny ecoli conjugation with FM433 ecoli
description: <strong>Tilt Series Date:</strong> 2014-10-25</p>
 
<strong>Data Taken By:</strong> Elitza Tocheva</p>
 
<strong>Species / Specimen:</strong> Escherichia coli</p>
 
<strong>Strain:</strong> NR1769 pKM101</p>
 
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å², defocus: -10.0 μm, magnification: 18000x. </p>
 
<strong>Microscope:</strong> Caltech Polara</p>
 
<strong>Acquisition Software:</strong> UCSFTomo</p>
 
<strong>Upload Method:</strong> reupload_downloaded</p>
 
<strong>Processing Software Used:</strong> IMOD</p>
 
<strong>Collaborators and Roles:</strong> Christian Baron</p>
 
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown on a gold grid in a drop of 1:1 mix of bacteria for 30min</p>
 
<strong>Sample Preparation:</strong> Vitrobot</p>
 
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/rawdata/</p>
 
NRpKMplusFM433_conj1_18K0007.mrc, Tilt Series (Pixel Size 0.63 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/rawdata/NRpKMplusFM433_conj1_18K0007.mrc" > <i class="download icon"></i> Download </a>
</p>
 
 NRpKMplusFM433_conj1_18K0007_full.rec, Reconstruction (Pixel Size 0.0 nm), 980.0 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/3dimage_49673/NRpKMplusFM433_conj1_18K0007_full.rec" > <i class="download icon"></i> Download </a>
</p>
 
 slicer000.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12321/slicer000.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer001.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12322/slicer001.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer002.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12323/slicer002.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer003.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12324/slicer003.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer004.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12325/slicer004.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer005.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12326/slicer005.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer006.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12327/slicer006.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer007.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12328/slicer007.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer008.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12329/slicer008.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer009.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12330/slicer009.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer010.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12331/slicer010.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer011.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12332/slicer011.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer012.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12333/slicer012.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer013.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12334/slicer013.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer014.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12335/slicer014.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer015.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12336/slicer015.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer016.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12337/slicer016.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer017.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12338/slicer017.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer018.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12339/slicer018.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer019.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12340/slicer019.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer020.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12341/slicer020.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer021.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12342/slicer021.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer022.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12343/slicer022.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer023.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12344/slicer023.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer024.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12345/slicer024.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer025.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12346/slicer025.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer026.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12347/slicer026.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer027.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12348/slicer027.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer028.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12349/slicer028.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer029.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12350/slicer029.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer030.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12351/slicer030.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer031.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12352/slicer031.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer032.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12353/slicer032.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer033.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12354/slicer033.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer034.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12355/slicer034.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer035.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12356/slicer035.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer036.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12357/slicer036.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer037.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12358/slicer037.tif" > <i class="download icon"></i> Download </a>
</p>
 
 slicer038.tif, 2.6 MB
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-10-25-12/file_12359/slicer038.tif" > <i class="download icon"></i> Download </a>
</p>