cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20624461
title: Design, synthesis, biological evaluation, and molecular docking of novel quinazolinone EGFR inhibitors as targeted anticancer agents

description: A novel series of 2-methyl-3-phenylquinazolin-4-one derivatives were synthesized and biologically evaluated for their cytotoxic potential against MCF-7, HepG2, and PC-3 cancer cells. Most of the tested compounds showed reasonable safety in the normal human skin melanocyte HFB4 cell line. Compound <b>4</b> showed potent cytotoxicity on Hep-G2 cell lines, while compound <b>9</b> showed potent cytotoxicity on the MCF-7 cell line, whereas compounds <b>10</b> and <b>12</b> showed potent cytotoxicity on Hep-G2 cell lines using 5-fluorouracil as a reference standard. Cell division analysis on the tested cell lines revealed that compounds <b>4</b>, <b>9</b>, <b>10</b>, and <b>12</b> have potent antiproliferative properties. An <i>in vitro</i> enzymatic inhibition assay against EGFR-TK confirmed that those compounds have potent EGFR inhibitory activity. The target compounds arrested the cell cycle at the pre-G1 and G2/M phases. Molecular docking simulations showed that all the target compounds possess a common binding pattern like that of Erlotinib.
DOI: 10.6084/m9.figshare.20624461.v1
title: Design, synthesis, biological evaluation, and molecular docking of novel quinazolinone EGFR inhibitors as targeted anticancer agents

description: A novel series of 2-methyl-3-phenylquinazolin-4-one derivatives were synthesized and biologically evaluated for their cytotoxic potential against MCF-7, HepG2, and PC-3 cancer cells. Most of the tested compounds showed reasonable safety in the normal human skin melanocyte HFB4 cell line. Compound <b>4</b> showed potent cytotoxicity on Hep-G2 cell lines, while compound <b>9</b> showed potent cytotoxicity on the MCF-7 cell line, whereas compounds <b>10</b> and <b>12</b> showed potent cytotoxicity on Hep-G2 cell lines using 5-fluorouracil as a reference standard. Cell division analysis on the tested cell lines revealed that compounds <b>4</b>, <b>9</b>, <b>10</b>, and <b>12</b> have potent antiproliferative properties. An <i>in vitro</i> enzymatic inhibition assay against EGFR-TK confirmed that those compounds have potent EGFR inhibitory activity. The target compounds arrested the cell cycle at the pre-G1 and G2/M phases. Molecular docking simulations showed that all the target compounds possess a common binding pattern like that of Erlotinib.
DOI: 10.57745/136vvq
title: 257480910275_201609151104_S01_GE1_107_Sep09_1_3.txt

description: :unav
DOI: 10.57745/aj6plx
title: 257480910288_201609231122_S01_GE1_107_Sep09_2_1.txt

description: :unav
DOI: 10.57745/r4oskt
title: 257480910287_201609231059_S01_GE1_107_Sep09_2_1.txt

description: :unav
DOI: 10.57745/xoioto
title: 257480910274_201609151041_S01_GE1_107_Sep09_1_4.txt

description: :unav
DOI: 10.57745/ycfkww
title: 257480910276_201609211054_S01_GE1_107_Sep09_2_1.txt

description: :unav
DOI: 10.57745/yqahyt
title: 257480910286_201609211117_S01_GE1_107_Sep09_1_2.txt

description: :unav
DOI: 10.57745/ffdg88
title: COSInet_formatted_data.rds

description: Normalized gene expression data
DOI: 10.57745/iwby5m
title: 257480910274_201609151041_S01_GE1_107_Sep09_1_2.txt

description: :unav
DOI: 10.57745/r0puzi
title: 257480910287_201609231059_S01_GE1_107_Sep09_2_4.txt

description: :unav
DOI: 10.57745/rvbjtj
title: Differential_results_final.xlsx

description: Results of differential expression analysis
DOI: 10.57745/8dgbdm
title: 257480910275_201609151104_S01_GE1_107_Sep09_2_1.txt

description: :unav
DOI: 10.57745/9v5ght
title: 257480910276_201609211054_S01_GE1_107_Sep09_2_3.txt

description: :unav
DOI: 10.57745/cod15h
title: COSI-net_final_analysis.pdf

description: Compiled Rmarkdown document for reproducing analyses
DOI: 10.57745/yhqp7n
title: 257480910287_201609231059_S01_GE1_107_Sep09_1_1.txt

description: :unav
DOI: 10.57745/yx56ng
title: 257480910274_201609151041_S01_GE1_107_Sep09_1_3.txt

description: :unav
DOI: 10.57745/9itutn
title: COSI-net_final_analysis.Rmd

description: Rmarkdown script for reproducing analyses
DOI: 10.57745/dox8sv
title: 257480910286_201609211117_S01_GE1_107_Sep09_2_1.txt

description: :unav
DOI: 10.57745/qkb9ye
title: 257480910275_201609151104_S01_GE1_107_Sep09_1_1.txt

description: :unav
DOI: 10.57745/ravzql
title: 257480910288_201609231122_S01_GE1_107_Sep09_2_3.txt

description: :unav
DOI: 10.57745/qvavo4
title: 257480910286_201609211117_S01_GE1_107_Sep09_1_4.txt

description: :unav
DOI: 10.57745/spzuq4
title: 257480910288_201609231122_S01_GE1_107_Sep09_1_2.txt

description: :unav
DOI: 10.57745/xkivua
title: exp_design.tab

description: Experimental design
DOI: 10.57745/hwb31x
title: 257480910288_201609231122_S01_GE1_107_Sep09_1_4.txt

description: :unav
DOI: 10.57745/tto7is
title: 257480910276_201609211054_S01_GE1_107_Sep09_2_4.txt

description: :unav
DOI: 10.57745/vq6xu3
title: 257480910287_201609231059_S01_GE1_107_Sep09_1_2.txt

description: :unav
DOI: 10.57745/xkkwfo
title: 257480910288_201609231122_S01_GE1_107_Sep09_1_3.txt

description: :unav
DOI: 10.57745/yljss1
title: 257480910274_201609151041_S01_GE1_107_Sep09_2_4.txt

description: :unav
DOI: 10.57745/4jaff3
title: Hallmark_results_final.xlsx

description: Results of hallmark gene set analysis
DOI: 10.57745/rbmdfo
title: 257480910287_201609231059_S01_GE1_107_Sep09_2_3.txt

description: :unav
DOI: 10.57745/rfehb0
title: 257480910276_201609211054_S01_GE1_107_Sep09_1_2.txt

description: :unav
DOI: 10.57745/uaebwo
title: 257480910275_201609151104_S01_GE1_107_Sep09_2_3.txt

description: :unav
DOI: 10.57745/wqgdjm
title: 257480910286_201609211117_S01_GE1_107_Sep09_2_4.txt

description: :unav
DOI: 10.6084/m9.figshare.21154948.v2
title: Anti-Tn antibody (cross-reactive human anti-A) formation, induced by aber-rant Tn antigen expressions, might be response to ubiquitous virus infections (A hypothesis)

description: The human innate anti-A isoagglutinin activity, expressed by polyreactive IgM, is serologically identical to the anti-A activity of C57BL/10 mice and is inhibited by <em>Helix pomatia</em>- and/or Tn-reactive ovarian glycolipids from these animals, which no longer produce the anti-A after early ovariectomy. Thus, the human anti-A antibody does not arise in response to A-allelic (genetic) antigenic activity. While the mouse anti-A antibody, produced together with <em>Helix pomatia</em>- and/or Tn-reactive glycolipids in the autologous ovary and mirrors the egg-protecting function of <em>Helix pomatia</em>-reactive anti-Tn hemagglutinin, the human anti-A most likely reflects nothing more than the monitoring of Tn-associated growth processes and can therefore be considered and referred to as anti-Tn antibody. Moreover, if different viral infections may be inhibited by natural and monoclonal anti-A antibodies, regardless of the infected individual’s ABO(H) blood group, it is unlikely that the stimulatory antigenicity is encoded by an A allele; and if additionally, viral serine molecules are essential for viral infections, this antigenicity is most likely a cross-species, mucin-type hybrid <em>O</em>-GalNAcα1-Ser/Thr-R or Tn formation. While a virus infection is thus initiated by hybrid Tn formation, such a formation also occurs by different pathways due to altered expression of glycan genes in chronic virus infection or virus-induced cancer, and the antibody responding to it is anti-Tn-specific, identical to the cross-reactive anti-A isoagglutinin, which ultimately represents another anti-Tn antibody version and a potential response to ubiquitous virus infections.
DOI: 10.6084/m9.figshare.20440581
title: Tn antibody the response to virus infections (A hypothesis)

description: The human innate anti-A isoagglutinin activity, expressed by polyreactive IgM, is serologically identical to the anti-A activity of C57BL/10 mice and is inhibited by <em>Helix pomatia</em>- and/or Tn-reactive ovarian glycolipids from these animals, which no longer produce the anti-A after early ovariectomy. Thus, the human anti-A antibody does not arise in response to A-allelic (genetic) antigenic activity. While the mouse anti-A antibody, produced together with <em>Helix pomatia</em>- and/or Tn-reactive glycolipids in the autologous ovary and mirrors the egg-protecting function of <em>Helix pomatia</em>-reactive anti-Tn hemagglutinin, the human anti-A most likely reflects nothing more than the monitoring of Tn-associated growth processes and can therefore be considered and referred to as anti-Tn antibody. Moreover, if different viral infections may be inhibited by natural and monoclonal antibodies, regardless of the infected individual’s ABO(H) blood group, it is unlikely that the stimulatory antigenicity is encoded by an A allele; and if additionally, viral serine molecules are essential for viral infections, this antigenicity is most likely a cross-species, mucin-type hybrid <em>O</em>-GalNAcα1-Ser/Thr-R or Tn formation, and the antibody responding to it is anti-Tn-specific, identical to the cross-reactive anti-A isoagglutinin, which ultimately represents another anti-Tn antibody version and a potential response to ubiquitous virus infections.
DOI: 10.6084/m9.figshare.20440581.v34
title: Tn antibody the response to virus infections (A hypothesis)

description: The human innate anti-A isoagglutinin activity, expressed by polyreactive IgM, is serologically identical to the anti-A activity of C57BL/10 mice and is inhibited by <em>Helix pomatia</em>- and/or Tn-reactive ovarian glycolipids from these animals, which no longer produce the anti-A after early ovariectomy. Thus, the human anti-A antibody does not arise in response to A-allelic (genetic) antigenic activity. While the mouse anti-A antibody, produced together with <em>Helix pomatia</em>- and/or Tn-reactive glycolipids in the autologous ovary and mirrors the egg-protecting function of <em>Helix pomatia</em>-reactive anti-Tn hemagglutinin, the human anti-A most likely reflects nothing more than the monitoring of Tn-associated growth processes and can therefore be considered and referred to as anti-Tn antibody. Moreover, if different viral infections may be inhibited by natural and monoclonal antibodies, regardless of the infected individual’s ABO(H) blood group, it is unlikely that the stimulatory antigenicity is encoded by an A allele; and if additionally, viral serine molecules are essential for viral infections, this antigenicity is most likely a cross-species, mucin-type hybrid <em>O</em>-GalNAcα1-Ser/Thr-R or Tn formation, and the antibody responding to it is anti-Tn-specific, identical to the cross-reactive anti-A isoagglutinin, which ultimately represents another anti-Tn antibody version and a potential response to ubiquitous virus infections.
DOI: 10.6084/m9.figshare.20440581.v33
title: Anti-Tn antibody might be response to virus infections (A hypothesis)

description: The human innate anti-A isoagglutinin activity, expressed by polyreactive IgM, is serologically identical to the anti-A activity of C57BL/10 mice and is inhibited by <em>Helix pomatia</em>- and/or Tn-reactive ovarian glycolipids from these animals, which no longer produce the anti-A after early ovariectomy. Thus, the human anti-A antibody does not arise in response to A-allelic (genetic) antigenic activity. While the mouse anti-A antibody, produced together with <em>Helix pomatia</em>- and/or Tn-reactive glycolipids in the autologous ovary and mirrors the egg-protecting function of <em>Helix pomatia</em>-reactive anti-Tn hemagglutinin, the human anti-A most likely reflects nothing more than the monitoring of Tn-associated growth processes and can therefore be considered and referred to as anti-Tn antibody. Moreover, if different viral infections may be inhibited by natural and monoclonal antibodies, regardless of the infected individual’s ABO(H) blood group, it is unlikely that the stimulatory antigenicity is encoded by an A allele; and if additionally, viral serine molecules are essential for viral infections, this antigenicity is most likely a cross-species, mucin-type hybrid <em>O</em>-GalNAcα1-Ser/Thr-R or Tn formation, and the antibody responding to it is anti-Tn-specific, identical to the cross-reactive anti-A isoagglutinin, which ultimately represents another anti-Tn antibody version and a potential response to ubiquitous virus infections.
DOI: 10.6084/m9.figshare.19165370
title: Tn-reactive immunoglobulin M (IgM) might form the first line of defense against SARS-CoV-2 infection (COVID-19): A proposed immunobiological background

description: The anti-Tn-reactive immunoglobulin M (IgM) might be identical to the nonimmune, germline-encoded blood group A-reactive isoagglutinin, expressed by the ancient poly- and autoreactive germline-encoded immunoglobulin M (IgM) in humans, which is serologically identical to anti-A from the C57BL/10 mouse and hardly arises in response to A-allelic genetic activities. This antibody, which does not occur in sera from blood group A individuals, may reflect the functions of metazoan defense proteins or lectins, which are considered non-self/self-recognition molecules that monitor expression of the cross-species evolutionary serological Tn antigen (<em>O</em>-GalNAcα1-Ser/Thr-R). Tn (T nouvelle) is an intermediate structure, almost exclusively expressed in tumor tissues and represents a key point of non-self-/self-recognition in metazoan species, while its complementary protein in human plasma, the anti-Tn-reactive IgM, mediates growth process monitoring. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) invades the human body via a trans-species Tn formation. The viral serine molecule, mobilized from the viral spike (S) protein by the host transmembrane protease serine subtype 2 (TMPRSS2) enzyme, was identified as a fusion molecule, essential for SARS-CoV-2 infection, wherein the syngeneic serine might be replaced by the pathogen molecule and the key point of nonself/self-recognition become the target of infection and source of aggressive autoimmunization. While it was argued that anti-Tn is protective against SARS-CoV-2, the hypothetical, cross-species enzyme-substrate competition between the host and viral serine molecule occurs preferentially in blood group A and the other non-O blood groups, whereas in blood group O(H), the foreignness of the hybrid Tn epitope is recognized as non-self by the corresponding anti-Tn-reactive IgM, which in concert with secondary anti-Tn-reactive immunoglobulin G (IgG) forms the first line of defense. <br>
DOI: 10.6084/m9.figshare.19165370.v48
title: Tn-reactive immunoglobulin M (IgM) might form the first line of defense against SARS-CoV-2 infection (COVID-19): A proposed immunobiological background

description: The anti-Tn-reactive immunoglobulin M (IgM) might be identical to the nonimmune, germline-encoded blood group A-reactive isoagglutinin, expressed by the ancient poly- and autoreactive germline-encoded immunoglobulin M (IgM) in humans, which is serologically identical to anti-A from the C57BL/10 mouse and hardly arises in response to A-allelic genetic activities. This antibody, which does not occur in sera from blood group A individuals, may reflect the functions of metazoan defense proteins or lectins, which are considered non-self/self-recognition molecules that monitor expression of the cross-species evolutionary serological Tn antigen (<em>O</em>-GalNAcα1-Ser/Thr-R). Tn (T nouvelle) is an intermediate structure, almost exclusively expressed in tumor tissues and represents a key point of non-self-/self-recognition in metazoan species, while its complementary protein in human plasma, the anti-Tn-reactive IgM, mediates growth process monitoring. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) invades the human body via a trans-species Tn formation. The viral serine molecule, mobilized from the viral spike (S) protein by the host transmembrane protease serine subtype 2 (TMPRSS2) enzyme, was identified as a fusion molecule, essential for SARS-CoV-2 infection, wherein the syngeneic serine might be replaced by the pathogen molecule and the key point of nonself/self-recognition become the target of infection and source of aggressive autoimmunization. While it was argued that anti-Tn is protective against SARS-CoV-2, the hypothetical, cross-species enzyme-substrate competition between the host and viral serine molecule occurs preferentially in blood group A and the other non-O blood groups, whereas in blood group O(H), the foreignness of the hybrid Tn epitope is recognized as non-self by the corresponding anti-Tn-reactive IgM, which in concert with secondary anti-Tn-reactive immunoglobulin G (IgG) forms the first line of defense. <br>
DOI: 10.6084/m9.figshare.19165370.v47
title: Anti-Tn-reactive immunoglobulin M (IgM) might form the first line of defense against SARS-CoV-2 infection (COVID-19): A proposed immunobiological background

description: The anti-Tn-reactive immunoglobulin M (IgM) might be identical to the nonimmune, germline-encoded blood group A-reactive isoagglutinin, expressed by the ancient poly- and autoreactive germline-encoded immunoglobulin M (IgM) in humans, which is serologically identical to anti-A from the C57BL/10 mouse and hardly arises in response to A-allelic genetic activities. This antibody, which does not occur in sera from blood group A individuals, may reflect the functions of metazoan defense proteins or lectins, which are considered non-self/self-recognition molecules that monitor expression of the cross-species evolutionary serological Tn antigen (<em>O</em>-GalNAcα1-Ser/Thr-R). Tn (T nouvelle) is an intermediate structure, almost exclusively expressed in tumor tissues and represents a key point of non-self-/self-recognition in metazoan species, while its complementary protein in human plasma, the anti-Tn-reactive IgM, mediates growth process monitoring. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) invades the human body via a trans-species Tn formation. The viral serine molecule, mobilized from the viral spike (S) protein by the host transmembrane protease serine subtype 2 (TMPRSS2) enzyme, was identified as a fusion molecule, essential for SARS-CoV-2 infection, wherein the syngeneic serine might be replaced by the pathogen molecule and the key point of nonself/self-recognition become the target of infection and source of aggressive autoimmunization. While it was argued that anti-Tn is protective against SARS-CoV-2, the hypothetical, cross-species enzyme-substrate competition between the host and viral serine molecule occurs preferentially in blood group A and the other non-O blood groups, whereas in blood group O(H), the foreignness of the hybrid Tn epitope is recognized as non-self by the corresponding anti-Tn-reactive IgM, which in concert with secondary anti-Tn-reactive immunoglobulin G (IgG) forms the first line of defense. <br>
DOI: 10.5281/zenodo.7113665
title: IG receptor germline set for species: Mouse subgroup: 129S1/SvImJ set_name: 129S1/SvlmJ IGLV

description: IG Germline Reference set published on the Open Germline Receptor Database (OGRDB)
DOI: 10.6084/m9.figshare.20145605.v3
title: Improving food security and livelihoods of smallholder farmers through mitigation of aflatoxins in animal feed

description: File contains the raw data extracted from studies/trials that were used for network meta-analysis
DOI: 10.57745/abrfu6
title: data_R_LC50_EC50.tab

description: 3 sheets : i/ abbreviations, ii/LC50, iii/EC50
DOI: 10.5519/qd.a3aje6f5
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (3544 records)
DOI: 10.5281/zenodo.7113679
title: IG receptor germline set for species: Mouse subgroup: A/J set_name: A/J IGKV

description: Germline Reference set published on the Open Germline Receptor Database (OGRDB)
DOI: 10.5281/zenodo.7113678
title: IG receptor germline set for species: Mouse subgroup: A/J set_name: A/J IGKV

description: Germline Reference set published on the Open Germline Receptor Database (OGRDB)
DOI: 10.5519/qd.eap1f0yn
title: Query on the Natural History Museum Data Portal (data.nhm.ac.uk) (4673 records)
DOI: 10.5281/zenodo.7113669
title: threskiornis_aethiopicus_decontaminated_FSCR.fasta.gx

description: .
DOI: 10.5281/zenodo.7113668
title: threskiornis_aethiopicus_decontaminated_FSCR.fasta.gx

description: .
DOI: 10.57745/7vf9ry
title: 20130124_VG_LMD9_ST.mzML

description: LC-MS/MS analysis after cell surface shaving of the strain Streptococcus thermophilus LMD9
DOI: 10.57745/lt9ppz
title: 20130124_VG_LH77.mzML

description: LC-MS/MS analysis after cell surface shaving of Lactobacillus helveticus CIRM-BIA99 (also referred to as ITGLH77)
DOI: 10.57745/bdrlfm
title: 20130124_VG_LH32.mzML

description: LC-MS/MS analysis after cell surface shaving of Lactobacillus helveticus CIRM-BIA103 (also referred to as CNRZ32 or LH32)
DOI: 10.5281/zenodo.7105973
title: TLS data of forest plot

description: TLS data acquired with a Riegl VZ-2000i within a forest plot in the Zirbenwald near Obergurgl, including 23 scanning positions using the one-touch-mode. Ground classification was done based on progressive TIN densification (Axelsson 2000). Projection: UTM 32N (EPSG 32632), fine registration based on the ALS-data from 2017.
DOI: 10.5281/zenodo.7105974
title: TLS data of forest plot

description: TLS data acquired with a Riegl VZ-2000i within a forest plot in the Zirbenwald near Obergurgl, including 23 scanning positions using the one-touch-mode. Ground classification was done based on progressive TIN densification (Axelsson 2000). Projection: UTM 32N (EPSG 32632), fine registration based on the ALS-data from 2017.
DOI: 10.5281/zenodo.7113687
title: IG receptor germline set for species: Mouse subgroup: A/J set_name: A/J IGLV

description: Germline Reference set published on the Open Germline Receptor Database (OGRDB)
DOI: 10.5281/zenodo.7113686
title: IG receptor germline set for species: Mouse subgroup: A/J set_name: A/J IGLV

description: Germline Reference set published on the Open Germline Receptor Database (OGRDB)
DOI: 10.57745/6xtpje
title: all_QTL2.qza

description: Subset of raw demultiplexed sequences (QIIME2 format)
DOI: 10.57745/fztoan
title: all_QTL4.qza

description: Subset of raw demultiplexed sequences (QIIME2 format)
DOI: 10.57745/mzfu3i
title: all_QTL3.qza

description: Subset of raw demultiplexed sequences (QIIME2 format)
DOI: 10.57745/qyhwqz
title: all_QTL1.qza

description: Subset of raw demultiplexed sequences (QIIME2 format)
DOI: 10.57745/09pg81
title: Donnees2_OTU_rawTable_Bayes.Rdata

description: ASV raw Table (Bayes algorithm) - Rdata format
DOI: 10.57745/bq2whl
title: all_QTL5.qza

description: Subset of raw demultiplexed sequences (QIIME2 format)
DOI: 10.57745/ebuehm
title: Donnees2_Bourran_Table_OTUs_brute_Level7_Vsearch.tab

description: ASV raw Table (VSearch algorithm)
DOI: 10.57745/uhn81s
title: Donnees2_OTU_rawTable_Vsearch.Rdata

description: ASV raw Table (VSearch algorithm) - Rdata format
DOI: 10.57745/z5yncn
title: Donnees2_Bourran_Table_OTUs_brute_Level7.tab

description: ASV raw Table (Bayes algorithm)
DOI: 10.57745/387hhi
title: tabulated-ITS1_all_QTL_metadata.qzv

description: Taxonomic Boxplot (Bayes algorithm) - QIIME2 format
DOI: 10.57745/axwwe8
title: Donnees2_OTU_filteredTable_Bayes.Rdata

description: ASV filtered Table (Bayes algorithm) - Rdata format
DOI: 10.57745/se3b57
title: Bourran_analysis.txt

description: README file
DOI: 10.57745/tye75z
title: table.qza

description: Raw ASV Table (QIIME2 format)
DOI: 10.57745/xhzen6
title: tabulated-ITS1_all_QTL_metadata_Vsearch.qzv

description: Taxonomic Boxplot (VSearch algorithm) - QIIME2 format
DOI: 10.15454/mu0hxx/62pkhl
title: Xylem_Stem_Root_Leaf_Trait data.tab

description: :unav
DOI: 10.15454/mu0hxx/doh1px
title: Xylem_Stem_Root_Leaf_Trait data-1.tab

description: :unav
DOI: 10.57745/wfq9la
title: Gungqi_Zhang_BMC_ Xylem_Stem_Root_Leaf_Trait data.tab

description: :unav
DOI: 10.15454/lsipkt/dclpfm
title: Survival_Step1.R

description: R script for pre-imaginal survival analysis (step 1) : estimation of the number of eggs from pixel data. Required data: Manually counted eggs and pixel numbers (Data_BlackEggs.csv) and Survival experiment (Data_survival-all.csv)