DOI: 10.5061/dryad.sr595
title: Data from: Age of heart disease presentation and dysmorphic nuclei in patients with LMNA mutations

description: Nuclear shape defects are a distinguishing characteristic in
 laminopathies, cancers, and other pathologies. Correlating these defects
 to the symptoms, mechanisms, and progression of disease requires unbiased,
 quantitative, and high-throughput means of quantifying nuclear morphology.
 To accomplish this, we developed a method of automatically segmenting
 fluorescently stained nuclei in 2D microscopy images and then classifying
 them as normal or dysmorphic based on three geometric features of the
 nucleus using a package of Matlab codes. As a test case, cultured
 skin-fibroblast nuclei of individuals possessing LMNA splice-site mutation
 (c.357-2A&gt;G), LMNA nonsense mutation (c.736 C&gt;T, pQ246X) in
 exon 4, LMNA missense mutation (c.1003C&gt;T, pR335W) in exon 6,
 Hutchinson-Gilford Progeria Syndrome, and no LMNA mutations were analyzed.
 For each cell type, the percentage of dysmorphic nuclei, and other
 morphological features such as average nuclear area and average
 eccentricity were obtained. Compared to blind observers, our procedure
 implemented in Matlab codes possessed similar accuracy to manual counting
 of dysmorphic nuclei while being significantly more consistent. The
 automatic quantification of nuclear defects revealed a correlation between
 in vitro results and age of patients for initial symptom onset. Our
 results demonstrate the method's utility in experimental studies of
 diseases affecting nuclear shape through automated, unbiased, and accurate
 identification of dysmorphic nuclei.
description: Fig3_All_and_Defective_StatisticsSigma-Plot spreadsheet with the data for Figure 3 of the manuscript. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.Fig3_All_and_Defective_Statistics_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for Figure 3 of the manuscript. )Fig4a_DataSigma-Plot file with data for figure 4a of the manuscript. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.Fig4a_Data_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for figure 4a of the manuscript.)Fig4b_DataSigma-plot data file for figure 4b in the manuscript. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.Fig4b_data_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for figure 4b in the manuscript.)FigS5Sigma-plot file with data for Fig. S5. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.FigS5_ExportAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for Fig. S5. )Fig3_GraphsSigma plot file containing data for other all families shown in Fig. 3. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.JasonPaper_Fig3_RedoGraphs.JNBFigure3-All_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for all families shown in Fig. 3. )JasonPaper_Fig3_RedoGraphs_Excel.xlsxLaminAC StatisticsSigma Plot Data file containing the data for comparing lamin and dapi stains. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.LaminAC Statistics_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for comparing lamin and dapi stains. )Validation StatsSigma-Plot file containing data for validation statistics in Table 1. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.Validation Stats_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for validation statistics in Table 1.)3D-PLOT-DATA-Fig2Sigma-plot file containing data for Fig. 2 3D plots. An excel file containing just the data-sheets is also provided under the same name for those with no access to jnb files.3D-PLOT-DATA.JNB3D-PLOT-DATA_Fig2_ExcelAn excel file containing just the data-sheets provided under the same name for those with no access to jnb files. (Data for Fig. 2 3D plots.)3D-PLOT-DATA_Excel.xlsx