cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/g3j86e
title: Non-indigenous macroalgal species in coralligenous habitats of the Marine Protected Area Isole Ciclopi (Sicily, Italy)

description: This dataset contains the digitized treatments in Plazi based on the original journal article Costanzo, Luca Giuseppe, Marletta, Giuliana, Alongi, Giuseppina (2021): Non-indigenous macroalgal species in coralligenous habitats of the Marine Protected Area Isole Ciclopi (Sicily, Italy). Italian Botanist 11: 31-44, DOI: http://dx.doi.org/10.3897/italianbotanist.11.60474, URL: http://dx.doi.org/10.3897/italianbotanist.11.60474
DOI: 10.6084/m9.figshare.14288549
title: ANTIMICROBIAL SUSCEPTIBILITY OF SAMPLES OF ESCHERICHIA COLI ISOLATED FROM INDUSTRIAL CHICKENS (GALLUS GALLUS DOMESTICUS LINNAEUS, 1758) WITH COLIBACILLOSIS

description: ABSTRACT The present study evaluated the susceptibility of Escherichia coli samples isolated from poultry with colisepticemia in midwestern São Paulo State at the Ornitopathology Laboratory of FMVZ-UNESP/Botucatu, SP. A large number of samples were found that were multi-resistant to the antibiotics tested, the less effective drugs being sulfonamide and tetracycline. All samples were susceptible to norfloxacin and gentamicin.
DOI: 10.6084/m9.figshare.14288547
title: ASSESSMENT OF INTERCURRENCE OF ENZOOTIC LEUCOSIS AND BOVINE TUBERCULOSIS IN DAIRY COWS IN THE STATE OF PERNAMBUCO, BRAZIL

description: ABSTRACT We carried out this study to evaluate the intercurrence of tuberculosis and enzootic leukosis in dairy cattle herds in 8 districts of the State of Pernambuco, Brazil, by establishing the prevalence of bovines reagent to specific diagnostic tests, and to also verify the correlation between enzootic leucosis, cattle tuberculosis, the leukocytes count and lymphocytes count among the cattle studied. A total of 662 animals were tested serologically and hematologically, by the techniques of double immunodiffusion in agarose gel and the white blood cell count with the total and differential leukocyte count, respectively, and 612 were evaluated immunoallergically by the tuberculin test. In the 15 herds examined, the prevalence of cattle that were positive to the AGID and tuberculin tests were 32.2% (213/662) and 14.1% (86/612), respectively. Analyzing the correlation between the variables total leukocytes and lymphocytes, we found that total leukocyte is highly related to the variable lymphocytes (72%). The results in this study indicated that the diseases studied are widespread in the population studied, with significant levels of growth in leukemia, and infections of bovine leukosis virus (VLB) and Mycobacterium bovis are active and growing, with risk of impairment of the health of cattle herds, and, due to the zoonotic nature of bovine tuberculosis, of the health of people who work with cattle.
DOI: 10.6084/m9.figshare.14288546
title: FRUIT FLIES (DIPTERA: TEPHRITIDAE), THEIR PARASITOIDS AND HOSTS IN VIÇOSA, A FOREST ZONE IN THE STATE OF MINAS GERAIS, BRAZIL

description: ABSTRACT This paper presents the species diversity of fruit flies (Tephritidae), their parasitoids and hosts in the county of Viçosa, Minas Gerais State. Brazil. McPhail traps containing hydrolyzed protein were hung in three areas with several crop species, and in a natural reserve of the Atlantic Rain Forest. Ripe fruits on plants and fallen fruits were collected to obtain fruit fly and parasitoid adults. Sixteen fruit fly species were captured: Ceratitis capitata (Wiedemann), Anastrepha bezzi Lima, A. bistrigata Bezzi, A. dissimilis Stone, A. distincta Greene, A. fraterculus (Wiedemann), A. furcata Lima, A. grandis (Macquart), A. manihoti Lima, A. minensis Lima, A. montei Lima, A. obliqua (Macquart), A. pseudoparallela (Loew), A. pickeli Lima, A. serpentina (Wiedemann) and A. sororcula Zucchi. A. furcata is reported for the first time in Minas Gerais state. Furthermore, the parasitoids Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan, Utetes anastrephae (Viereck) (Braconidae) and Aganaspis pelleranoi (Brèthes) (Figitidae) were obtained, along with 4 specimens of Pteromalidae. This is the first report of these parasitoids in the region of Viçosa, and of O. bellus in Minas Gerais State.
DOI: 10.6084/m9.figshare.14288544
title: DIVERSITY AND CONTROL OF PEST INSECTS IN THE JUDICIAL ARCHIVE OF THE CITY COUNCIL OF PITANGUI, STATE OF MINAS GERAIS, BRAZIL

description: ABSTRACT Destruction of books and documents by pest organisms is an age-old problem, faced mainly by both librarians and archivists. The present study provides a brief report on the main actions and the results of the Integrated Management of Pests (IMP) implemented in the Judicial Archive of the City Council of Pitangui, state of Minas Gerais, Brazil, during the months of January 2004 to August 2005. These actions included a survey of the pest organisms present both in the physical area of the archive and in the bibliographical material. All the material was treated through the controlled freezing method, which consistsin putting the bibliographical material into a freezer at negative temperatures for a predetermined time period. In the case of this method, the effectiveness of the control was evaluated through the verification of the presence of dead and living individuals, soon after the treatment and at three and six months later. The survey of the pest organisms in the physical area of the archive revealed the presence of the termite Cryptotermes brevis (Walker) and the ants Tapinoma melanocephalum (Fabricius) and Camponotus sp. In the bibliographical material, in addition to the termite C. brevis there were identified beetles of the species Tricorynus herbarius (Gorham), silverfish Lepisma saccharina Linnaeus, and book lice of the genus Liposcelis sp. The control measures evidenced the effectiveness of the methods used, with no reoccurrence of the pest during the study period.
DOI: 10.6084/m9.figshare.14288543
title: DEVELOPMENT OF PERIPLANETA AUSTRALASIAEFABRICIUS (BLATTODEA, BLATTIDAE) AT A CONSTANT TEMPERATURE OF 30° C AND AT AMBIENT LABORATORY TEMPERATURE

description: ABSTRACT With the objective of obtaining standards of measurement for prevention and control, this study compared the development of the eggs and nymphs of Periplaneta australasiae (Fabricius) at a temperature of 30 ± 0.2° C, relative humidity 80 ± 15% and photoperiod of 12 hours versus ambient conditions in the laboratory without controls of temperature and RH. Single ootheca were maintained in test tubes until ecolosion, and nymphs were transferred to glass cubes and fed commercial rabbit ration and water ad libitum until emergence of the imagos. The incubation period, number of eggs/ ootheca, viability of the eggs, number of nymphs/ootheca, nymphal duration, viability of nymphs and duration of egg to adult were all evaluated. The mean difference in the incubation period between the temperature of 30° C (38 days) and ambient conditions (44.5 days) was significant (p &lt; 0.0001); a mean of 18.1 nymphs/ootheca ecloded at 30° C, while 21 nymphs/ootheca ecloded under ambient conditions (p = 0.006); the mean nymphal period at 30° C was 155.9 days while for the ambient it was 279.7 days (p &lt; 0.0001); nymphal viability was greater than 50% for both the 30° C laboratory (55.1%) and the ambient (57.2%); and the mean period from egg to adult of P. australasiae was significantly different (p &lt; 0.001) between the 30° C temperature (194.1 days) and the ambient conditions of the laboratory (337.3 days). Under ambient conditions, the duration of nymphal incubation and egg to adult development of P. australasiae were increased relative to the temperature of 30° C without a reduction in viability in any of the stages.
DOI: 10.6084/m9.figshare.14288541
title: SUSCEPTIBILITY OF RHYNCHOPHORUS PALMARUM TO THE ACTION OF METARHIZIUM ANISOPLIAE AND THE COMPATIBILITY OF THE ENTOMOPATHOGEN WITH AGROCHEMICALS USED IN THE BANANA CROP

description: ABSTRACT This study was aimed to screen the entomopathogenic fungus Metarhizium anisopliae for the control of Rhynchophorus palmarum (L.) (Coleoptera: Curculionidae) adults and to evaluate the compatibility of agrochemicals used in this crop with the strain that caused the highest insect mortality. To this end, adult insects were immersed in suspensions of 6 strains at a concentration of 109 conidia.mL-1, and after 24 days the isolate IBCB 348 caused the highest mortality (87.5%) and was used in the compatibility test, using calculations by way of the T formula, with commercial insecticides, herbicides and fungicides used in the crop. Among the tested strains, IBCB 348 was the most efficient in the control of this pest, causing 87.5% of weevil mortality. None of the tested fungicides and herbicides were compatible with the pathogen. The insecticides Calypso, Dipterex 500 and Sevin 480 SC, at the lowest dose recommended by the manufacturer, were the only products classified as compatible with the strain IBCB 348 of M. anisopliae.
DOI: 10.6084/m9.figshare.14288540
title: SELECTION AND MOLECULAR CHARACTERIZATION OF BACILLUS THURINGIENSIS BERLINER WITH TOXIC ACTIVITY AGAINST TRICHOPLUSIA NI HUEBNER (LEPIDOPTERA: NOCTUIDAE)

description: ABSTRACT Trichoplusia ni is a polyphagous pest that is becoming a major pest in plantations of cruciferous crops, soybeans and cotton. This study was aimed to select and molecularly characterize efficient isolates of Bacillus thuringiensis (Bt) for the control of T. ni. For the bioassays of pathogenicity, an aliquot with a 3 x 108 spores/mL suspension of Bt of each isolate was applied on the surface of the artificial diet disk, previously distributed on acrylic plates with 50 larvae, distributed in 5 repetitions. In bioassays to obtain the LC50, only isolates with 100% mortality were preselected, and tests were carried out at the concentrations 102, 5 x 102, 103, 2 x 103, 4 x 103, 6 x 103, and 8 x 103 spores/mL, and the treatments consisting of 120 larvae, distributed in 3 repetitions. A molecular characterization was performed to detect the genes cry1, cry2 and Vip for the isolates which obtained mortality over 95%. Isolates HD-1 (Standard), Bt-1043N-V, Bt-1034F, Bt-1009K, Bt-1000 and Bt-969A caused 100% mortality in the test for pathogenicity and presented an LC50 of 1.17 x 103, 1.45 x 103, 1, 46 x 103, 1.01 x 103, 9.43 x 102, 1.22 x 103, respectively. Genes cry1, cry2 and Vip were not found in the isolates tested, and other Cry toxins may have been causing the mortality of T. ni, since the isolates tested are specific for the Lepidoptera order. These isolates showed potential for the control of T. ni, being aggressive to this insect, with a potential to be used in a pest management program for this species.
DOI: 10.6084/m9.figshare.14288537
title: SEMI-SOLID PRODUCTION OF METARHIZIUM ANISOPLIAE (METSCH.) SOROK AND BEAUVERIA BASSIANA (BALS.) VUILL ON DIFFERENT MEDIA AND THE EFFECTS OF ULTRAVIOLET RADIATION AND TEMPERATURE ON THE INFECTIVE PROPAGULES OF THESE FUNGI

description: ABSTRACT The present study was aimed to evaluate different (semi-solid) media for the production of Metarhizium anisopliae and Beauveria bassiana propagules, and to evaluate the tolerance of these propagules to ultraviolet radiation and temperature. The experiments were performed at the Biological Control Laboratory of the Instituto Biológico at Campinas, São Paulo, Brazil. For both fungi, 6 repetitions were performed for each of the 17 treatments: corn starch, full rice, parboiled rice, type-1 rice, type-2 rice, oat flakes, canjiquinha [grits], wheat flour, raw cassava flour, yellow corn flour, special wheat flour, corn flour, corn in grains, cassava starch, soy in grains, crushed wheat, and turf. The viability analysis was done in plastic plates containing BDA. For the bioassays involving exposure to ultraviolet light and temperature, BDA was also used for viability analysis, and each treatment was exposed to the UV radiation for 0, 25 and 50 seconds, the temperature exposure being at 20, 25, 30 and 35º C. Using a Potter tower, 2 mL of fungus suspension from each treatment was inoculated to the Diatraea saccharalis caterpillars. Regarding the sporulation, the largest concentrations of M. anisopliae and B. bassiana were found for the treatments with parboiled rice, type-1 rice, type-2 rice, yellow corn flour, corn flour and crushed wheat. The viability of all treatments was superior to 94.00%. Also, the longer the duration of the exposition to the UV, the 2Universidade Estadual Paulista, Faculdade de Ciências Agronômicas, Botucatu, SP, Brasil.
DOI: 10.6084/m9.figshare.14288536
title: EFFECTIVENESS OF A NEW COMMERCIAL INSECTICIDE FOR THE CONTROL OF THE LESSER MEALWORM (ALPHITOBIUS DIAPERINUS) (PANZER) (COLEOPTERA: TENEBRIONIDAE)

description: ABSTRACT The present study was carried out with the objective to evaluate, under laboratory and field conditions, the effectiveness of the insecticide Test (based on cypermethrin, chlorpyrifos and citronellal), for the control of lesser mealworm (Alphitobius diaperinus). In the laboratory, the product was tested on adult insects, by spraying on the insects (direct contact) and by their walking on a treated surface (indirect contact). After this, it was evaluated in field conditions, in a poultry house. The product was effective, being comparable to the standard product, causing 100% of mortality in the laboratory and reducing the population in the poultry house by 76%. Thus, the product presents an alternative for the control of this pest.
DOI: 10.6084/m9.figshare.14288534
title: PRODUCTIVE AND SANITARY ASPECTS OF CROSSBRED MILKING COWS RECEIVING HOMEOPATHIC PRODUCTS

description: ABSTRACT Nowadays the world population demands food of good quality, without any residues from chemical products. Eight cows from a herd of 40, which were consuming commercial homeopathic medicines at the daily concentration for control of endoand ectoparasites, were monitored in terms of their performance (production and quality of milk, body score and body weight) and sanitary aspects (parasites infestation and mastitis) during the lactation period. It was not necessary to give any allopathic (conventional) medicines to control mastitis or parasites (ticks, horn-fly, verminosis) in any cow of the herd during the nine months of the lactation period. The results showed that it is possible to produce milk without using chemical products, and not interfering in the productivity of the crossbred animals.
DOI: 10.6084/m9.figshare.14288532
title: CLONING AND EXPRESSION IN PICHIA PASTORIS OF THE S1 GLYCOPROTEIN GENE OF THE CHICKEN INFECTIOUS BRONCHITIS VIRUS

description: ABSTRACT Genetic and antigenic variation are very frequently observed among IBV strains and affect mainly the S1 glycoprotein. In order to contribute to the availability of tools for immunodiagnosis and immunoprophylaxis of chicken infectious bronchitis we developed an expression system for production of recombinant S1 glycoprotein in Pichia pastoris. We obtained the cDNA from viral RNA on embryonated eggs infected with the M41 strain of IBV, by reverse transcription (RT) and polymerase chain reaction (PCR), amplifying the S1 coding sequence with extremities compatible with the vector used to transform yeast. Induction with methanol led to the production of a protein with the predicted molecular weight that was detected by Western blot in the cell lysate of transformed yeast. Expression in P. pastoris proved to be an effective method for recombinant production of S1 protein from IBV, with potential for use in immuno-diagnosis of chicken infectious bronchitis virus.
DOI: 10.6084/m9.figshare.14288530
title: IN VITRO EFFECT ON PHYTOPATHOGENS OF ESSENTIAL OIL AND AQUEOUS CRUDE EXTRACT OF OCIMUM GRATISSIMUM HARVESTED IN THE FOUR SEASONS

description: ABSTRACT The present &gt;work aimed to evaluate the effects of the essential oil and crude extract of wild basil (Ocimum gratissimum), harvested in different seasons, on the in vitro mycelia growth of Rhizoctonia solani, Sclerotium rolfsii, Phytophthora sp. and Alternaria alternata. To evaluate the effect of the essential oil, sterilized oil aliquots of 20 ?L, 40 ?L and 60 ?L were distributed on the surface of potato-dextroseagar (PDA). The aqueous crude extracts (ACE) were filtrated and incorporated in PDA at 1%, 5%, 10%, 15%, 20%, 25% and 50%. The results showed total inhibition of mycelial growth in the different aliquots of essential oil. The ACE collected from plants in autumn had better results; in this season the ACE at 5% was enough to provide 100% of mycelia growth inhibition of A. alternata and S. rolfsii.
DOI: 10.6084/m9.figshare.14288528
title: OVIPOSITION PREFERENCE AND ITS RELATION TO THE PERFORMANCE OF DIAPHANIA HYALINATA (L., 1758) (LEPIDOPTERA: CRAMBIDAE) IN CUCURBITS.

description: ABSTRACT Diaphania hyalinata L. (Lepidoptera, Pyralidae) stands out as a major pest of the Cucurbitaceae family. This insect is oligophagous with a polyphagous habit, which arouses wide interest in studies of their behavioral ecology for reasons of pest management in crops of high economic importance such as muskmelon (Cucumis melo L.). Given this situation, basic studies aimed at an understanding of ecological aspects of this pest are needed to improve pest management systems, especially in the aspect of population monitoring. Given this, the present study was aimed to determine whether the choice of the host exerted by females of D. hyalinata corresponds to the best performance of the offspring and how the hosts influence their population dynamics. Oviposition-choice tests were performed on melon, watermelon, cucumber, loofah, balsam apple and cucumber, to establish a ranking of preference. For the performance of larvae and reproduction the melon was chosen (among the most preferred hosts) along with loofah and cucumber (among the least preferred hosts). The results support the hypothesis that the choice of host is related to the performance of the offspring and D. hyalinata has demonstrated plasticity in dealing with different hosts which may be indicative of ability to adapt to fluctuating food resources in terms of quality and availability. However, generally this plasticity is expensive, especially in reproductive terms.
DOI: 10.6084/m9.figshare.14288526
title: IN VITRO EVALUATION OF THE SUSCEPTIBILITY OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS TO DIFFERENT KINDS OF ANTISEPTICS AND DISINFECTANTS

description: ABSTRACT The aim of this study was to evaluate the in vitro effect of antiseptics and disinfectants against Corynebacterium pseudotuberculosis, and to define the growth curve of this microrganism inoculated in brain heart infusion broth plus 0.1% of Tween 80 (BHI + T), for 48 hours incubation. For the susceptibility test, evaluations were made using 10% iodine, 2.5% sodium hypochlorite, 5% potassium permanganate, Aseptol® liquid soap, and absolute ethyl alcohol (99.8%), by way of the disc-diffusion method. A sterile swab was immersed in a bacterial suspension and plated in Mueller-Hinton agar. Sterile discs were immersed in each solution to be tested and distributed on the agar surface. The results were obtained according to the inhibition circle diameter formed around the disc. For the growth curve determination, colonies were inoculated in a bottle containing BHI + T. Every 4 hours, 2 mL was withdrawn to evaluate the cell mass in a spectrophotometer, and 1 mL was taken to perform serial dilutions, blood agar base plating and counting of viable cells. It was observed that in order to reach the maximum concentration of C. pseudotuberculosis, close to 1,200 x 105 viable cells/mL, the inoculum must be maintained at appropriate incubation for a period of 28-40 hours. The sensibility test indicated
DOI: 10.6084/m9.figshare.14288523
title: SEROLOGIC INQUIRY OF TOXOPLASMA GONDII IN SHEEP IN NORTHERN BRAZIL

description: ABSTRACT Toxoplasmosis is a serious zoonosis and widespread parasitic disease. A serologic inquiry in Santo Antonio do Tauá and Santa Izabel do Pará counties in the Castanhal Microregion, state of Pará, Northern Brazil, demonstratedahighfrequency,(44.29%) ofanti-Toxoplasmagondii antibodies detected by indirect hemaglutination test, with a distribution at titers 1:64, 1:128, 1:256, 1:512, 1:1024 and 1:2048 of, respectively, 50 (32.26%), 42 (27.10%), 29 (18.70%), 11 (7.10%), 12 (7.74%) and 11(7.10%). A statistically significant difference was observed between the 0 up to 2 and &gt; 2 age groups in both sexes (?2 = 13.42, p = 0.0002) with a greater frequency in the &gt; 2 years age group, (58.71%), and between the sexes (?2 = 23.51, p = 0.0001) with a greater frequency in females, (72.26%). Although the two counties presented similar frequencies (49.33% and 40.50% for Santa Izabel do Pará and Santo Antonio do Tauá, respectively) no association was observed between them. Sanitary surveillance measures are recommended to prevent health risks to humans and animals.
DOI: 10.6084/m9.figshare.14288522
title: DAMAGES CAUSED BY MASTITIS IN SANTA INÊS BRAZILIAN SHEEP BREED

description: ABSTRACT In meat sheep herds in Brazil, mastitis has provoked many damages, causing mortality and decreasing the numbers of lambs born and weaned, posing heavy economic impacts for the production system. The objective of this study was to quantify the damages caused by mastitis in a Santa Inês breed herd, reared in a semi-intensive production system, in São Paulo State, Brazil. Clinical exams were conducted and the California mastitis test was carried out, and milk samples were taken from the ewes for microbiological assay. The lambs were weighed at birth and on the days when the milk samples were taken. Forty-one ewes gave birth to 55 lambs (birth rate of 1.34) during the parturition season from 23 October 2008 to 4 January 2009. Twenty (36%) of them died, 14 (70%) in the first 2 weeks following birth, and 6 (30%) before weaning, which occurs at about 70 days. A total of 70% (14) of the deaths were imputed to the occurrence of mastitis in the respective ewe, and 30% (6) to other causes, mainly pneumonia. Mastitis decreased the average daily gain of the lambs from birth to weaning (P &lt; 0.10). Most of the positive microbiological samples (85.8%) where positive for Staphylococcus spp.
DOI: 10.6084/m9.figshare.14288520
title: MICROBIOLOGICAL PROFILE OF FISH AND POND WATER IN FEE-FISHING LOCATED IN THE NORTHEAST REGION OF SÃO PAULO STATE, BRAZIL

description: ABSTRACT The objective of this study was to determine the number of total and thermotolerant coliforms, coagulase-positive Staphylococcus, and the presence of bacteria of the genus Salmonella in the muscle, surface tissue, and gastrointestinal tract of fish, and in the pond water of fee-fishing establishments located in the Córrego Rico microwatershed in the state of São Paulo, Brazil. Coagulase-positive Staphylococcus was not detected in any sample of pond water or fish. The most probable number (MPN) of total coliforms in fish ranged from 2.0 x 10 to &gt; 1.1 x 104 NMP.100 mL-1, while in pond water it ranged from 4.2 x 104 to &gt; 2.4 x 105 NMP.100 mL-1. The count of thermotolerant coliforms in fish ranged from &lt; 3 to 5.1 x 103 NMP.g-1, while in pond water it ranged from 3.8 x 102 to 2.0 x 104 NMP.100 mL-1. There was no statistical difference (P &gt; 0.05) among the studied populations of microorganisms in pond water, surface tissue and gastrointestinal tract, which reflects a relationship between the presence of microorganisms in the water and in the two tissues analyzed. Salmonella sp. was isolated in one sample of muscle and in two samples of gastrointestinal tract. The fish can be a vehicle for cross contamination, having the gastrointestinal tract and the surface tissue as a source of microorganisms for its muscles. Therefore, monitoring the water quality is important to ensure the production of fish with good quality.