cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/588mj-x5a64
title: Agrobacterium tumefaciens C58

description: Agro C58
description: <strong>Tilt Series Date:</strong> 2013-10-23</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Induction for T-pilus with 200uM AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-4/rawdata/</p>c58_berk_home_195k0004.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-4/rawdata/c58_berk_home_195k0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_berk_home_195k0004.rec,  Reconstruction (Pixel Size 0.0 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-23-4/3dimage_49038/c58_berk_home_195k0004.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t8saa-qhk12
title: Azo delta Operon 4

description: Azospirillum Delta Chemotaxis Operon 4
description: <strong>Tilt Series Date:</strong> 2014-10-27</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Azospirillum brasilense</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Gladys Alexandre</p>
<strong>Purification / Growth Conditions / Treatment:</strong> rGow overnight in 5 mL MMAB+N (on bench) without shaking- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N - Leave at RT (on bench) without shaking overnight</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-27-40/rawdata/</p>AzoDeltaOp4_7.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-27-40/rawdata/AzoDeltaOp4_7.mrc"
        > <i class="download icon"></i> Download </a></p>

        AzoDeltaOp4_7_part121_57.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-27-40/3dimage_47747/AzoDeltaOp4_7_part121_57.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cx137-y0950
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-13</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-4/rawdata/</p>12mar13_c121_1_Cos74ADN_AT4bt1_0006.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-4/rawdata/12mar13_c121_1_Cos74ADN_AT4bt1_0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar13_c121_1_Cos74ADN_AT4bt1_0006_full.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-13-4/3dimage_17727/12mar13_c121_1_Cos74ADN_AT4bt1_0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y3khe-2ch22
title: MJ sacculi

description: Skinny B. subtilis MJ007 sacculi of sporulating cells
description: <strong>Tilt Series Date:</strong> 2012-03-02</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kit Pogliano</p>
<strong>Purification / Growth Conditions / Treatment:</strong> see Mol Micro Tocheva et al reference</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-15/rawdata/</p>mjsac20015.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-15/rawdata/mjsac20015.mrc"
        > <i class="download icon"></i> Download </a></p>

        mjsac20015_full.rec,  Reconstruction (Pixel Size 3.8 nm), 825.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-03-02-15/3dimage_17363/mjsac20015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/k96eb-j2g63
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN and pNLGFP provirus construct
description: <strong>Tilt Series Date:</strong> 2012-04-25</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-13/3dimage_68556/</p>12apr25_C123_3_Cos7pNLDN_AT3BT1_0010_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-13/3dimage_68556/12apr25_C123_3_Cos7pNLDN_AT3BT1_0010_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12apr25_C123_3_Cos7pNLDN_AT3BT1_0010.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-13/rawdata/12apr25_C123_3_Cos7pNLDN_AT3BT1_0010.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/551hc-1fg18
title: Agrobacterium tumefaciens

description: Bald strain, flagellin A, B and C missing. D is present
description: <strong>Tilt Series Date:</strong> 2013-10-30</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on AB medium and induced with AS</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-12/rawdata/</p>bald20011.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-12/rawdata/bald20011.mrc"
        > <i class="download icon"></i> Download </a></p>

        bald20011_full.rec,  Reconstruction (Pixel Size 1.6 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-30-12/3dimage_33676/bald20011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x80h2-41c61
title: JPA 1558 LMEM

description: JPA 1558 TlpT - YFPlabelled cytoplasmic receptor correlative cryo-LMECT experiment (LM from 051512)
description: <strong>Tilt Series Date:</strong> 2012-05-16</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-44.0°, 56.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -12.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in Sux media at 30 degrees, Ampicillin treated for one hour (2.57ml sux plus 0.0113g Ampicillin)membrane dye FM64X added 10 min at room temp before antibiotic treatment for one hour</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-4/rawdata/</p>1573_LMEM4.mrc,  Tilt Series (Pixel Size 0.78 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-4/rawdata/1573_LMEM4.mrc"
        > <i class="download icon"></i> Download </a></p>

        1573_LMEM4_part102_3.rec,  Reconstruction (Pixel Size 1.56 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-05-16-4/3dimage_18635/1573_LMEM4_part102_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qte2k-0q641
title: Streptococcus pneumoniae

description: over expression of SP_0107 PG hydroalse
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Streptococcus pneumoniae</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Andrew Camili</p>
<strong>Purification / Growth Conditions / Treatment:</strong> over expression of SP_0107 PG hydroalse</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-16/rawdata/</p>overexpr0016.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-16/rawdata/overexpr0016.mrc"
        > <i class="download icon"></i> Download </a></p>

        overexpr0016_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-09-16/3dimage_30564/overexpr0016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.95k78r
title: Occurrence Download

description: A dataset containing 16165 species occurrences available in GBIF matching the query:
{
 "and" : [
 "DatasetKey is one of (EOD – eBird Observation Dataset, iNaturalist Research-grade Observations)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "Geometry POLYGON((-80.4399 43.45367,-80.37948 43.45367,-80.37948 43.4859,-80.4399 43.4859,-80.4399 43.45367))"
 ]
}

 The dataset includes 16165 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047693-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/6sx56-jbk20
title: HUVECs infx pNLBg1I_endptvirus

description: Raw data files of HUVECs infx pNLBg1I_endptvirus
description: <strong>Tilt Series Date:</strong> 2013-11-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> pNLGFPBg1I obtained from Irvin Chen (UCLA)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Whole cells were grown on Au finder grids and infected with pNLGFPBg1I. Sample was frozen after endpoint of virus infection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-7/rawdata/</p>13nov04_Endptvirus_montage_21x21tgt_0003.mrc,  Tilt Series (Pixel Size 0.56 nm), 2.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-7/rawdata/13nov04_Endptvirus_montage_21x21tgt_0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        13nov04_Endptvirus_montage_21x21tgt_0003_part81_13.rec,  Reconstruction (Pixel Size 2.24 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-11-04-7/3dimage_33785/13nov04_Endptvirus_montage_21x21tgt_0003_part81_13.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.frgr4g
title: Occurrence Download

description: A dataset containing 10 species occurrences available in GBIF matching the query:
{
 "and" : [
 "OccurrenceStatus is Present",
 "TaxonKey is Pectis monocephala"
 ]
}

 The dataset includes 10 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047695-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/r82m5-zmd23
title: Agrobacterium tumefaciens

description: Agrao VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-08</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-29/rawdata/</p>agro_vg3101_70001.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-29/rawdata/agro_vg3101_70001.mrc"
        > <i class="download icon"></i> Download </a></p>

        agro_vg3101_70001.rec,  Reconstruction (Pixel Size 1.68 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-08-29/3dimage_34355/agro_vg3101_70001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2ae36-rs030
title: Agrobacterium tumefaciens

description: C58 grown on slide
description: <strong>Tilt Series Date:</strong> 2013-10-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on a glass slide</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-1/rawdata/</p>c58_slide_2750009.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-1/rawdata/c58_slide_2750009.mrc"
        > <i class="download icon"></i> Download </a></p>

        c58_slide_2750009_full.rec,  Reconstruction (Pixel Size 1.56 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-10-04-1/3dimage_31917/c58_slide_2750009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mzyc8-hmq35
title: Agro_C58_22C_liq

description: Raw data files of Agro_C58_22C_liq
description: <strong>Tilt Series Date:</strong> 2014-09-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> strain from Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 22C liquid</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-09-05-22/rawdata/</p>C58_liq_22C40002.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-09-05-22/rawdata/C58_liq_22C40002.mrc"
        > <i class="download icon"></i> Download </a></p>

        C58_liq_22C40002_part121_5.rec,  Reconstruction (Pixel Size 0.0 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-09-05-22/3dimage_49719/C58_liq_22C40002_part121_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9wa65-cct06
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-23/3dimage_69405/</p>12may29_C135_1_MEFnomitoDNA_AT2BT20014_part82_51.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
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        12may29_C135_1_MEFnomitoDNA_AT2BT20014.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
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