DOI: 10.57760/sciencedb.j00217.00887
title: Analysis of the correlation between the expression of the topoisomerase IIA(TOP2A) gene and the abundance of CD4+T cells and its corresponding immune mechanism in epithelial ovarian carcinoma.
title: 拓扑异构酶ⅡA( TOP2A) 基因在卵巢癌细胞中的表达与CD4+T细胞的数量相关性及免疫机制的探究

description: Objective To analyze the correlation between the expression of topoisomerase Ⅱα(TOP2A) gene and the number of CD4+T cells in epithelial ovarian cancer (EOC) and its immune mechanism.Methods The expression of TOP2A mRNA in normal ovarian tissue and EOC tissue, as well as its prognostic significance for EOC patients, were analyzed using the Gene Annotation Resource Database (BioGPS), gene expression profile interaction analysis (GEPIA), and Kaplan-Meier Plotter databases. The Human Protein Atlas database was utilized to analyze the expression of TOP2A protein in ovarian cancer and normal ovarian tissues. Co-expressed genes of TOP2A, along with their gene ontology (GO) and pathway enrichment analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG), were examined using GENE and Metascape databases. Furthermore, the relationship between the TOP2A gene and CD4+T cells, cell subsets, various immune cells was investigated using TISIDB, ImmuCellAI, and TIMER databases. Additionally, the impact of CD4+T cell distribution on survival and prognosis of patients with EOC was assessed.Results The expression of TOP2A mRNA and protein was not significant in normal ovarian tissue and CD4+T cells, but was significantly high in EOC tissue, which was not conducive to the survival prognosis of patients. The GO function of TOP2A co-expressed genes is mainly concentrated in the mitotic cell cycle G0 and early G1 division phase, PID-E2F pathway and transcriptional regulation of TP53, while KEGG is mainly concentrated in metabolic regulation, positive/negative feedback regulation of biological processes, stimulus response, cell cycle and cell growth and development. The expression of TOP2A gene was significantly correlated with the purity of tumor cells and the number of CD4+T cells and multiple immune cells. A variety of immune cells have certain copy number variation in EOC, among which deep deletion is the most significant. However, only the number of CD8+T cells infiltrated by tumor microenvironment had a significant impact on survival and prognosis of EOC patients.Conclusion The expression of TOP2A mRNA in EOC tissues is positively correlated with the number of CD4+T cells, and the low distribution of tumor-infiltrating CD8+T cells is not conducive to the survival prognosis of EOC patients.
description: Objective To analyze the correlation between the expression of topoisomerase Ⅱα(TOP2A) gene and the number of CD4+T cells in epithelial ovarian cancer (EOC) and its immune mechanism.Methods The expression of TOP2A mRNA in normal ovarian tissue and EOC tissue, as well as its prognostic significance for EOC patients, were analyzed using the Gene Annotation Resource Database (BioGPS), gene expression profile interaction analysis (GEPIA), and Kaplan-Meier Plotter databases. The Human Protein Atlas database was utilized to analyze the expression of TOP2A protein in ovarian cancer and normal ovarian tissues. Co-expressed genes of TOP2A, along with their gene ontology (GO) and pathway enrichment analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG), were examined using GENE and Metascape databases. Furthermore, the relationship between the TOP2A gene and CD4+T cells, cell subsets, various immune cells was investigated using TISIDB, ImmuCellAI, and TIMER databases. Additionally, the impact of CD4+T cell distribution on survival and prognosis of patients with EOC was assessed.Results The expression of TOP2A mRNA and protein was not significant in normal ovarian tissue and CD4+T cells, but was significantly high in EOC tissue, which was not conducive to the survival prognosis of patients. The GO function of TOP2A co-expressed genes is mainly concentrated in the mitotic cell cycle G0 and early G1 division phase, PID-E2F pathway and transcriptional regulation of TP53, while KEGG is mainly concentrated in metabolic regulation, positive/negative feedback regulation of biological processes, stimulus response, cell cycle and cell growth and development. The expression of TOP2A gene was significantly correlated with the purity of tumor cells and the number of CD4+T cells and multiple immune cells. A variety of immune cells have certain copy number variation in EOC, among which deep deletion is the most significant. However, only the number of CD8+T cells infiltrated by tumor microenvironment had a significant impact on survival and prognosis of EOC patients.Conclusion The expression of TOP2A mRNA in EOC tissues is positively correlated with the number of CD4+T cells, and the low distribution of tumor-infiltrating CD8+T cells is not conducive to the survival prognosis of EOC patients.