DOI: 10.5061/dryad.3bk3j9kr6
title: Phylogeny and tribal classification of Australian Prioninae (Coleoptera: Cerambycidae)

description: We applied whole genome shotgun sequencing to museum specimens of longhorn
 beetles and reconstructed the phylogenetic relationships of Australian
 Prioninae using mitochondrial protein-coding genes. Based on the molecular
 phylogeny and morphological characters, we suggest downgrading the
 subfamily Parandrinae Blanchard to the tribe Parandrini in the Prioninae
 Latreille and synonymise Erichsoniini Thomson with Parandrini Blanchard.
 Australian Prioninae are classified in seven tribes: Catypnini Lacordaire
 stat. rev., Mocrotomini Thomson, Osphryonini trib. nov., Parandrini
 Blanchard, Rhipidocerini trib. nov., Sceleocanthini Lacordaire stat. rev.,
 and Tereticini Lameere. Tribal compositions and generic relationships are
 tested, and the subtribal divisions within the large tribe Macrotomini are
 briefly discussed.
description: A total of 174 pinned specumens (57 from Australia and 117 from
 outside Australia) were sampled for this study. DNA was extracted from leg
 tissue using the DNeasy 96 Blood and Tissue Kit protocol (Qiagen) and
 measured with a Qubit fluorometer (Thermo Fisher). Libraries were prepared
 following the QIAseq FX DNA Library Kit protocol (Qiagen) with one-thrid
 reaction volumes. The sample DNA was fragmented with the FX Enhancer for
 15 minutes, and a right-side size selection was performed at the adaptor
 ligation cleanup stage. Samples were triple-indexed using custom adapters
 based on standard Illumina TruSeq UDI adaptors. Individual libraries were
 pooled equally and sequenced on a NovaSeq6000 S1 with 150 bp PE at the
 Biomolecular Resource Facility, Australian National University.
 Sequencing raw reads were trimmed by Trimmomatic v.0.36 and de
 novo assembled using Spades v.3.11.1. Putative mitochondrial (mt) contigs
 were recognized using BLAST and a reference dataset containing 25 complete
 mt-genomes of Australian prionines, then blasted against NCBI GenBank and
 against each other to remove contaminants. mt-PCGs were annotated based on
 references and extracted accordingly, aligned at the translated amino acid
 level using Muscle and concatenated to a final supermatrix. Final
 nucleotide sequence (NT) and amino acid sequence (AA) alignments were
 partitioned by gene positions and analysed in ModelFinder with +MERGE
 option. Phylogenetic relationships were inferred under Maximum Likelihood
 criterion in IQTREE2 v.2.0.3. We performed 20 independent tree searches
 for AA and NT datasets, respectively, and selected the tree with the best
 likelihood score. Nodal support values were estimated using the slow
 standard nonparametric bootstrap in IQ-TREE2 with 200
 replicates.
description: # Phylogeny and tribal classification of Australian Prioninae (Coleoptera:
 Cerambycidae)
 [https://doi.org/10.5061/dryad.3bk3j9kr6](https://doi.org/10.5061/dryad.3bk3j9kr6) ## Description of the data and file structure This data package contains the sequence alignments and phylogenetic trees from Jin et al., Phylogeny and tribal classification of Australian Prioninae (Coleoptera: Cerambycidae), Annales zoologici, (Warsaw), 2023, 73(3), 499-512. Corresponding author: Mengjie Jin; jinmengj@mail2.sysu.edu.cn; mengjie.jin@csiro.au (CSIRO) All files including: * Samples been sequenced in this study: AppendixS1\_sample info.xlsx; * Alignment files of 13 mitochondrial protein-coding genes(used to generate phylogenies): AppendixS4\_NTalignment.nex (concatenated alignment of nucleotides); * Maximum likelihood phylogenies from IQ-TREE2 v2.0.3 (http://www.iqtree.org/): AppendixS2\_treefile.Prioninae.aa.bs200.tre (Newick-format tree from amino acid dataset, partitioned by gene position); * AppendixS3\_treefile.Prioninae.nt.bs200.tre (Newick-format tree from nucleotide dataset, partitioned by gene position). All files edited for Dryad were initially uploaded on 17 Aug 2023, README\_file.txt was uploaded on 17 Aug 2023 and updated on 28 Sep 2023. AppendixS1\_sample info.xlsx was edited on 28 Sep 2023 for adding on GenBank Accession IDs. ## Sharing/Access information NCBI GenBank Accession IDs for all sequences that are used for phylogenetic reconstructions are provided in AppendixS1 and can be retrieved from https://www.ncbi.nlm.nih.gov/genbank/ accordingly.