DOI: 10.5061/dryad.8931zcs4n
title: Data from: Urinary signatures are associated with calorie restriction-mediated weight loss in obese Diversity Outbred mice

description: Metabolomic profiles are increasingly being used to identify responders to
 dietary interventions. Advances using this approach are particularly
 needed to personalize and enhance the effectiveness of dietary weight loss
 interventions. Using obese Diversity Outbred (DO) mice that model genetic
 and phenotypic heterogeneity of human populations, we aimed to identify
 urinary metabolite signatures associated with responsiveness to calorie
 restriction (CR)-mediated weight loss. DO mice (150 males, 150 females)
 were fed a high-fat diet for 12 weeks to induce obesity, then urine was
 collected and an 8-week CR regimen (30 % decrease in energy intake)
 initiated. At study completion, mice were rank-ordered according to their
 percent body weight change, with mice in the extreme quartiles deemed CR
 responders (n = 67) versus nonresponders (n = 67). Targeted
 semi-quantitative metabolomics identified elevated glutamic acid and
 hydroxyproline as key urinary metabolites that distinguish CR responders
 from CR nonresponders, independent of sex. Three urinary metabolites
 (glutamic acid, hydroxyproline, and putrescine) distinguished male CR
 responders from nonresponders. Six metabolites (glutamic acid,
 hydroxyproline, dopamine, histamine, lysine, and spermine) distinguished
 female CR responders from nonresponders. Multivariate receiver operating
 characteristic analyses integrated these metabolites to reveal potential
 sex specific and sex-independent associations of CR-mediated weight loss.
 Further, pathway analysis identified several metabolic pathways, including
 arginine and proline metabolism, and alanine, aspartate, and glutamate
 biosynthesis, that distinguished CR responders from nonresponders and
 could be indicative of metabolic reprogramming to enhance insulin
 sensitivity and energy metabolism.
description: # Data from: Urinary signatures are associated with calorie
 restriction-mediated weight loss in obese Diversity Outbred mice Dataset
 DOI: [10.5061/dryad.8931zcs4n](https://doi.org/10.5061/dryad.8931zcs4n) ##
 Description of the data and file structure Targeted semiquantitative
 metabolomics from biobanked urine samples from obese calorie restriction
 responders (CR-Responders) and calorie restriction nonresponders
 (CR-Nonresponders) Diversity Outbred mice collected immediately before the
 calorie restriction intervention. ### Files and variables #### File:
 All_CR-R_vs_CR-NR.csv **Description:** Urinary metabolite concentrations
 of male and female CR-responders and CR-nonresponders. ##### Variables *
 ID: M_# * CR-responder: Calorie restriction responder * CR-nonresponder:
 Calorie restriction nonresponder * NA: Missing (not detected) #### File:
 Female_CR-R_vs_CR-NR.csv **Description:**  ##### Variables *
 **Description:** Urinary metabolite concentrations of female CR-responders
 and CR-nonresponders. ##### Variables * ID: M_# * CR-responder: Calorie
 restriction responder * CR-nonresponder: Calorie restriction nonresponder
 * NA: Missing (not detected) #### File: Male_CR-R_vs_CR-NR.csv
 **Description:**  ##### Variables * **Description:** Urinary metabolite
 concentrations of male CR-responders and CR-nonresponders. ##### Variables
 * ID: M_# * CR-responder: Calorie restriction responder * CR-nonresponder:
 Calorie restriction nonresponder * NA: Missing (not detected) ##
 Code/software None. ## Access information Data was derived from the
 following sources: * Data were generated from mouse urine samples analyzed
 with the Biocrates AbsoluteIDQ® p180 targeted metabolomics assay.