DOI: 10.5061/dryad.r246hv0
title: Data from: Prior reproduction alters how mitochondria respond to an oxidative event

description: An animal's pace of life is mediated by the physiological demands and
 stressors it experiences (e.g., reproduction) and one likely mechanism
 that underlies these effects is oxidative stress. Reproduction has been
 shown to increase or reduce oxidative stress under different conditions
 and modify mitochondrial performance. We hypothesized that the changes
 associated with reproduction can alter how animals respond to future
 oxidative stressors. We tested this theory by comparing the organ-specific
 mitochondrial response in female wild-derived house mice. Specifically, we
 compared mice that reproduced or were virgins to mice that were exposed to
 an oxidant (i.e., radiation) or not-exposed to radiation. We measured
 liver and skeletal muscle mitochondrial density, respiratory performance,
 enzyme activity, and oxidant production, as well as markers of oxidative
 damage to tissues. In the liver, prior reproduction prevented a
 radiation-induced reduction in mitochondrial density and increased
 mitochondrial respiratory performance. In skeletal muscle, prior
 reproduction resulted in a radiation-induced decline in mitochondrial
 density which could reduce the bioenergetic capacity of skeletal muscle
 mitochondria. Yet, electron transport chain complex I activity in skeletal
 muscle, which dropped with reproduction, returned to control levels
 following oxidant exposure. The results of this investigation indicate
 that prior reproduction alters the response of mitochondria to an
 oxidative challenge in an organ-specific manner. Such changes could have
 differential effects on future reproductive performance and risk of death.
description: R Code for Hood et al
 2019 X-ray Repro StudyThis file provides the R
 code for all analysesCode for Hood et al 2019 X-ray
 Repro Study_revised March 29
 2019.RData Hood et
 alThis file includes all
 data analyzed. A list of abbreviations can be found in the manuscript
 supplement.start.dat
 3-29-19.csvReproductive output data
 Hood et alThis file includes all
 data used to look at the impact of reproductive output on the variables
 quantifying in this study. A list of abbreviations can be found in the
 supplement to the manuscript.Repro only dat March
 29.csvData summary Hood et
 alThis file includes all
 of the summary data in this study. A list of abbreviations can be found in
 the supplement to the manuscript.summary repro dat March
 29.csvFinal Data Summary_g
 March 29This file includes all
 results of the hedges g calculations. A list of abbreviations can be found
 in the supplement to the manuscript.GLS Model Estimates
 March 29This file includes all
 results of the generalize least squares model for the adult females. A
 list of abbreviations can be found in the supplement to the
 manuscript.GLS Pup Model Results
 March 29This file includes all
 results of the generalize least squares model for the pups. A list of
 abbreviations can be found in the supplement to the
 manuscript.Linear Model Estimates
 March 29This file includes all
 results of the linear model for the adult females. A list of abbreviations
 can be found in the supplement to the manuscript.