DOI: 10.5061/dryad.bk3j9kdjf
title: Data from: Phase-dependent differential in vitro and ex vivo susceptibility of Aspergillus flavus and Fusarium keratoplasticum to azole antifungals

description: Fungal keratitis (FK) is an invasive infection of the cornea primarily
 associated with Aspergillus and Fusarium species. FK is treated
 empirically with a limited selection of topical antifungals with varying
 levels of success. Though clinical infections are typically characterized
 by a dense network of mature mycelium, traditional models used to test
 antifungal susceptibility of FK isolates exclusively evaluate
 susceptibility in fungal cultures derived from asexual spores known as
 conidia. The purpose of this study was to characterize differences in
 fungal response when topical antifungal treatment is initiated at
 progressive phases of fungal development. We compared efficacy of
 voriconazole and luliconazole against in vitro cultures of A. flavus and
 F. keratoplasticum at 0, 24, and 48 h of fungal development. Data
 from this assay is included here, reported as end-point absorbance
 values. A porcine cadaver corneal model was used to compare
 antifungal efficacy of voriconazole and luliconazole in ex vivo tissue
 cultures of A. flavus and F. keratoplasticum at 0, 24, and 48 h of fungal
 development. Data from this assay may be found here, reported as pixel
 counts of the determined area.
description: <em>In vitro</em> absorbance: Absorbance at 490 nm
 was measured following assay conclusion (Sunrise Microplate Reader, Tecan
 Group Ltd, Switzerland). A sample size of three wells (n=3) was included
 for each fungal/drug combination. Raw absorbance per well is
 reported.  <em>Ex vivo</em> corneal
 coverage: Corneas were photographed following assay conclusion (Nikon
 D200, AF-S FX Micro NIKKOR 105mm 1:2.8G ED Lens). Images were analyzed
 using ImageJ software to outline the total area/cornea and the affected
 area/cornea in pixels. Raw total and affected areas/cornea are reported
 alongside the proportion of affected to total area. 
description: # Title of Dataset: Phase-dependent differential *in vitro* and *ex vivo*
 susceptibility of *Aspergillus flavus* and *Fusarium keratoplasticum* to
 azole antifungals. Absorbance of 96-well fungal culture is reported as a
 diagnostic of broth microdilution antifungal susceptibility testing.
 Corneal affected area is reported in pixel counts as a diagnostic of an
 *ex vivo* antifungal susceptibility model. ## Description of the Data and
 file structure Absorbance data is separated by fungal species, drug tested
 (VOR: voriconazole, LUL: luliconazole), and sample group. Sample groups
 include Control (no drug received), 0 h Incubation (drug received after 0
 h incubation), 24 h Incubation (drug received after 24 h incubation), and
 48 h Incubation (drug received after 48 h incubation). Absorbance values
 are reported as absorbance units (AU) measured at 490 nm. Each value
 represents a measurement taken from a single well of a 96-well plate.
 Corneal coverage data is separated into equivalent fungal species, drug,
 and sample group columns. Sample groups are further divided into three
 measurements: affected area, total area, and proportion. Each set of
 measurements represents a single sample. Area values are reported as raw
 pixel counts obtained from ImageJ photo analysis. Proportion values are
 obtained through mathematical transformation using Microsoft Excel.