DOI: 10.6084/m9.figshare.1480461.v8
title: Group functional template generated with BASC on cognitively normal elderly and mild cognitive impairment populations

description: <b># Content</b>This work is derived from the Alzheimer's Disease Neuroimaging Initiative 2 (ADNI2) and three samples from Montreal, Canada, as described in the following publication: Tam et al, Common effects of amnestic mild cognitive impairment on resting-state connectivity across four independent studies (dx.doi.org/10.3389/fnagi.2015.00242). It includes group brain parcellations for clusters generated from resting-state functional magnetic resonance images for 99 cognitively normal elderly persons and 129 patients with mild cognitive impairment. The parcellations have been generated using a method called bootstrap analysis of stable clusters (BASC, Bellec et al., 2010) and 8 resolutions of clusters (4, 6, 12, 22, 33, 65, 111, and 208 total bihemispheric parcels) were selected using a data-driven method called MSTEPS (Bellec, 2013). This work also includes parcellations that contain regions-of-interest (ROIs) that span only one hemisphere at 8 resolutions (10, 17, 30, 51, 77, 137, 199, and 322 total ROIs). It also includes maps illustrating uncorrected functional connectivity differences (t-maps) between patients and controls for four seeds/ROIs (superior medial frontal cortex, dorsomedial prefrontal cortex, striatum, middle temporal lobe). This release contains the following files: * <b>README.md</b>: a text description of the release. * <b>brain_parcellation_mcinet_basc_(sym,asym)_(#</b><b>)</b><b>clusters.(mnc,nii).gz</b>: 3D volumes (either in .mnc or .nii format) at 3 mm isotropic resolution, in the MNI non-linear 2009a space (http://www.bic.mni.mcgill.ca/ServicesAtlases/ICBM152NLin2009), at multiple resolutions of # clusters. Region number I is filled with Is (background is filled with 0s). Note that four versions of the templates are available, named with sym_mnc, asym_mnc, sym_nii or asym_nii. The mnc flavor contains files in the minc format, while the nii flavor has files in the nifti format. The asym flavor contains brain images that have been registered in the asymmetric version of the MNI brain template (reflecting that the brain is asymmetric), while with the sym flavor they have been registered in the symmetric version of the MNI template. The symmetric template has been forced to be symmetric anatomically, and is therefore ideally suited to study homotopic functional connections in fMRI: finding homotopic regions simply consists of flipping the x-axis of the template. Note: These clusters are often bihemispheric. For parcellations containing regions that span only one hemisphere, see below. * <b>brain_parcellation_mcinet_basc_(sym,asym)_(#)rois.(mnc,nii).gz</b>: 3D volumes (either in .mnc or .nii format) at 3 mm isotropic resolution, in the MNI non-linear 2009a space, at multiple resolutions of # ROIs, that span only one hemisphere. As above, mnc/nii and sym/asym versions of the templates are available.* <b>labels_mcinet_(sym,asym)_ (#)(clusters,ROIs).csv</b>: spreadsheets containing labels for each cluster or ROI * <b>ttest_ctrlvsmci_seed(#).(mnc,nii).gz</b>: 3D volumes (either in .mnc or .nii) displaying functional connectivity differences (uncorrected t-tests) between patients with mild cognitive impairment and cognitively normal elderly, for 4 different seeds/regions of interest i.e. striatum (seed #2), dorsomedial prefrontal cortex (#9), middle temporal lobe (#12), superior medial frontal cortex (#28); cluster numbers are taken from the parcellation containing 33 clusters. <b># Preprocessing</b> The datasets were analysed using the NeuroImaging Analysis Kit (NIAK https://github.com/SIMEXP/niak) version 0.12.18, under CentOS version 6.3 with Octave (http://gnu.octave.org) version 3.8.1 and the Minc toolkit (http://www.bic.mni.mcgill.ca/ServicesSoftware/ServicesSoftwareMincToolKit) version 0.3.18. Each fMRI dataset was corrected for inter-slice difference in acquisition time and the parameters of a rigid-body motion were estimated for each time frame. Rigid-body motion was estimated within as well as between runs, using the median volume of the first run as a target. The median volume of one selected fMRI run for each subject was coregistered with a T1 individual scan using Minctracc (Collins and Evans, 1998), which was itself non-linearly transformed to the Montreal Neurological Institute (MNI) template (Fonov et al., 2011) using the CIVET pipeline (Ad-Dabbagh et al., 2006). The MNI symmetric template was generated from the ICBM152 sample of 152 young adults, after 40 iterations of non-linear coregistration. The rigid-body transform, fMRI-to-T1 transform and T1-to-stereotaxic transform were all combined, and the functional volumes were resampled in the MNI space at a 3 mm isotropic resolution. The “scrubbing” method of (Power et al., 2012), was used to remove the volumes with excessive motion (frame displacement greater than 0.5 mm). A minimum number of 50 unscrubbed volumes per run was then required for further analysis. The following nuisance parameters were regressed out from the time series at each voxel: slow time drifts (basis of discrete cosines with a 0.01 Hz high-pass cut-off), average signals in conservative masks of the white matter and the lateral ventricles as well as the first principal components (95% energy) of the six rigid-body motion parameters and their squares (Giove et al., 2009). The fMRI volumes were finally spatially smoothed with a 6 mm isotropic Gaussian blurring kernel. <b># Bootstrap Analysis of Stable Clusters</b> Brain parcellations were derived using BASC (Bellec et al. 2010). A region growing algorithm was first applied to reduce the brain into regions of roughly equal size, set to 1000 mm3. The BASC used 100 replications of a hierarchical clustering with Ward's criterion on resampled individual time series, using circular block bootstrap. A consensus clustering (hierarchical with Ward's criterion) was generated across all the individual clustering replications pooled together, hence generating group clusters. The generation of group clusters was itself replicated by bootstrapping subjects 500 times, and a consensus clustering (hierarchical Ward's criterion) was generated on the replicated group clusters. The MSTEPS procedure (Bellec et al., 2013) was implemented to select a data-driven subset of scales in the range 5-200, approximating the group stability matrices up to 5% residual energy, through linear interpolation over selected scales. This resulted in 8 resolutions: 4, 6, 12, 22, 33, 65, 111, 208. Note that the number of resolutions was selected by the MSTEPS procedure in a data-driven fashion, and that the number of individual, group and final (consensus) number of clusters were not necessarily identical. These 8 resolutions were further processed to generate 8 parcellations that contain ROIs that span only one hemisphere. These latter parcellations contain 10, 17, 30, 51, 77, 137, 199, and 322 total ROIs. <b># Derivation of functional connectomes</b> For each resolution K, and each pair of distinct clusters, the between-clusters connectivity was measured by the Fisher transform of the Pearson’s correlation between the average time series of the clusters. The within-cluster connectivity was the Fisher transform of the average correlation between time series inside the cluster. An individual connectome was thus a KxK matrix. <b> # Statistical testing</b> To test for differences between patients and controls at a given resolution, we used a general linear model (GLM) for each connection between two clusters. The GLM included an intercept, the age and sex of participants, and the average frame displacement of the runs involved in the analysis. The contrast of interest (patients–controls) was represented by a dummy covariate coding the difference in average connectivity between the two groups. All covariates except the intercept were corrected to a zero mean. The GLM was estimated independently for each scanning protocol. The estimated effects were combined across all protocols through inverse variance based weighted averaging (Willer et al., 2010). <b># References</b> Ad-Dab’bagh, Y, et al, 2006. The CIVET Image-Processing Environment: A Fully Automated Comprehensive Pipeline for Anatomical Neuroimaging Research. In: Corbetta, M (Ed.), Proceedings of the 12th Annual Meeting of the Human Brain Mapping Organization. Neuroimage, Florence, Italy. Bellec, P, et al, 2010. Multi-level bootstrap analysis of stable clusters in resting-state fMRI. NeuroImage 51 (3), 1126–1139. Bellec, P, Jun. 2013. Mining the Hierarchy of Resting-State Brain Networks: Selection of Representative Clusters in a Multiscale Structure. In: Pattern Recognition in Neuroimaging (PRNI), 2013 International Workshop on. pp. 54–57. Collins, DL, Evans, AC, 1997. Animal: validation and applications of nonlinear registration-based segmentation. International Journal of Pattern Recognition and Artificial Intelligence 11, 1271–1294. Fonov, V, et al, 2011. Unbiased average age-appropriate atlases for pediatric studies. NeuroImage 54 (1), 313–327. Giove, F, et al, 2009. Images-based suppression of unwanted global signals in resting-state functional connectivity studies. Magnetic resonance imaging 27 (8), 1058–1064. Power, JD, et al, 2012. Spurious but systematic correlations in functional connectivity MRI networks arise from subject motion. NeuroImage 59 (3), 2142–2154. Willer, CJ, et al, 2010. METAL: fast and efficient meta-analysis of genomewide association scans. Bioinformatics 26, 2190–2191. <b># Other derivatives</b> The datasets that were used to generate the parcellations are described in a publication, see the following link: https://github.com/SIMEXP/mcinet