DOI: 10.5061/dryad.ksn02v7c8
title: Data from: Estimating bloodstain age in the short term based on DNA fragment length using nanopore sequencer

description: We used a nanopore sequencer to quantify DNA fragments &gt; 10,000 bp
 in size and then evaluated their relationship with short-term bloodstain
 age. Moreover, DNA degradation was investigated after bloodstains were
 wetted once with water. Bloodstain samples on cotton gauze were stored at
 room temperature and low humidity for up to 6 months. Bloodstains stored
 for 1 day were wetted with nuclease-free water, allowed to dry, and stored
 at room temperature and low humidity for up to 1 week. The proportion of
 fragments &gt; 20,000 bp in dry bloodstains tended to decrease over
 time, particularly for fragments &gt; 50,000 bp in size. This trend
 was modeled using a power approximation curve, with the highest R2 value
 (0.6475) noted for fragments &gt; 50,000 bp in size; lower values were
 recorded for shorter fragments. The proportion of longer fragments was
 significantly reduced in bloodstains that were dried after being wetted
 once, and there was significant difference in fragments &gt; 50,000 bp
 between dry conditions and once-wetted. This result suggests that even
 temporary exposure to water causes significant DNA fragmentation, but not
 extensive degradation. Thus, bloodstains that appear fresh but have a low
 proportion of long DNA fragments may have been wetted previously. Our
 results indicate that evaluating the proportion of long DNA fragments
 yields information on both bloodstain age and the environment in which
 they were stored.
description: These fastq files were used for analysis in this study. A file with a
 "*" after it indicates that the files contain DNA CS (DNA
 control strand; 3.6 kb standard amplicon mapping the 3' end of the
 Lambda genome) sequences for quality check. The read count percentage
 calculations were performed after excluding these sequences. A folder with
 a "w" prefix indicates that the fastq data are from the
 experiments (figure 2) in which the bloodstain was wetted with water.
 "Alignment summary" folder includes the results of mapping with
 hg38 by the MinKNOW software. Folder PATH listing | +---1day \|
 AJM454_pass_bc2cd138_0.fastq(*) \| ALB645_pass_20c53053_0.fastq(*) \|
 ALL990_pass_1a2f28eb_0.fastq(*) \|
 ANN476_pass_ed34f4ba_46227b6b_0.fastq(*) | +---2days \|
 ALB394_pass_929d1638_0.fastq(*) \| ALR682_pass_50fad96d_0.fastq(*) \|
 ANN386_pass_6e9ecc23_89da19c2_0.fastq(*) \|
 ANR037_pass_7afa0862_06d9d000_0.fastq(*) | +---3days \|
 AJM407_pass_ab643349_0.fastq(*) \| ALB405_pass_5c9ecc26_0.fastq(*) \|
 ANU671_pass_9e0f6da0_2ed6595f_0.fastq(*) \|
 ANX060_pass_0a211314_54dba230_0.fastq(*) | +---5days \|
 ALC054_pass_aa864ef9_0.fastq(*) \| ALL960_pass_d4836cab_0.fastq(*) \|
 AMX219_pass_03cca76e_c5c9e514_0.fastq \|
 ANK566_pass_ca4d85a9_79ee0342_0.fastq(*) | +---7days \|
 AJM455_pass_b8e0c36f_0.fastq(*) \| ALB710_pass_bc12d776_0.fastq(*) \|
 ALR175_pass_3dbed4ef_0.fastq(*) \|
 ANK215_pass_09b18fef_479fc77d_0.fastq(*) | +---10days \|
 ALC013_pass_557321c9_0.fastq(*) \|
 ANM449_pass_ec7b8135_0169951c_0.fastq(*) \|
 AOL145_pass_5ce9a6fb_ea26ef4a_0.fastq \|
 AOO261_pass_7a496160_90dccef5_0.fastq | +---14days \|
 AJO736_pass_8dda8496_0.fastq(*) \| ALB608_pass_9cd1060c_0.fastq(*) \|
 ALT654_pass_7ec3ce48_0.fastq(*) \|
 ANN273_pass_4cbce703_117b6910_0.fastq(*) | +---21days \|
 ALC072_pass_4f8beeab_0.fastq(*) \|
 ANT028_pass_7dbff7e2_d895ac13_0.fastq(*) \|
 ANU496_pass_7a62ef46_77febc71_0.fastq(*) \|
 AOL214_pass_de7e944e_b75bcd03_0.fastq | +---28days \|
 AJU547_pass_25edcf12_0.fastq(*) \| ALB270_pass_5a306b17_0.fastq(*) \|
 ALR652_pass_48dffbe9_0.fastq(*) \| AON611_pass_eb1217db_2344207a_0.fastq |
 +---2months \| ALM138_pass_22a42b73_0.fastq(*) \|
 AOL206_pass_922ab2db_04f992cf_0.fastq \|
 AOL534_pass_ccc161da_3d5166e1_0.fastq \|
 AOM261_pass_559ad00e_0ba2bf5b_0.fastq | +---3months \|
 ANG280_pass_1faa73da_0.fastq(*) \| ANO532_pass_6dfc9979_0.fastq(*) \|
 ANO756_pass_b7acb270_0.fastq(*) \| AOL584_pass_0540ecf7_ada5001a_0.fastq |
 +---4months \| ANG698_pass_253d9022_0.fastq(*) \|
 ANP242_pass_343f75a8_0.fastq(*) \| ANQ483_pass_48ae7bfa_0.fastq(*) \|
 AOT468_pass_8e792725_1286f3c4_0.fastq | +---6months \|
 ANG702_pass_54ea346b_0.fastq(*) \| ANH875_pass_0934c646_0.fastq(*) \|
 ANP446_pass_90fea4ca_0.fastq(*) \| AOV830_pass_91b38c3f_78d37fb2_0.fastq |
 +---w_1day \| FAW77777_pass_barcode08_8ca636cc_e83af5d2_0.fastq \|
 FAW81892_pass_barcode01_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode09_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode14_f810f4db_dc345a2f_0.fastq | +---w_2days \|
 FAW77777_pass_barcode09_8ca636cc_e83af5d2_0.fastq \|
 FAW81892_pass_barcode02_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode10_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode15_f810f4db_dc345a2f_0..fastq | +---w_3days \|
 FAW77777_pass_barcode10_8ca636cc_e83af5d2_0.fastq \|
 FAW81892_pass_barcode03_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode11_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode16_f810f4db_dc345a2f_0.fastq | +---w_5days \|
 FAW77777_pass_barcode11_8ca636cc_e83af5d2_0.fastq \|
 FAW81892_pass_barcode04_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode12_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode17_f810f4db_dc345a2f_0.fastq | +---w_8days \|
 FAW77777_pass_barcode12_8ca636cc_e83af5d2_0.fastq \|
 FAW81892_pass_barcode07_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode13_f810f4db_dc345a2f_0.fastq \|
 FAW81892_pass_barcode18_f810f4db_dc345a2f_0.fastq | +---alignment summary
 +---1 day \| AJM454_pass_bc2cd138_0.txt \| ALB645_pass_20c53053_0.txt \|
 ALL990_pass_1a2f28eb_0.txt \| ANN476_pass_ed34f4ba_46227b6b_0.txt | +---2
 days \| ALB394_pass_929d1638_0.txt \| ALR682_pass_50fad96d_0.txt \|
 ANN386_pass_6e9ecc23_89da19c2_0.txt \| ANR037_pass_7afa0862_06d9d000_0.txt
 | +---3 days \| AJM407_pass_ab643349_0.txt \| ALB405_pass_5c9ecc26_0.txt
 \| ANU671_pass_9e0f6da0_2ed6595f_0.txt \|
 ANX060_pass_0a211314_54dba230_0.txt | +---5 days \|
 ALC054_pass_aa864ef9_0.txt \| ALL960_pass_d4836cab_0.txt \|
 AMX219_pass_03cca76e_c5c9e514_0.txt \| ANK566_pass_ca4d85a9_79ee0342_0.txt
 | +---7 days \| AJM455_pass_b8e0c36f_0.txt \| ALB710_pass_bc12d776_0.txt
 \| ALR175_pass_3dbed4ef_0.txt \| ANK215_pass_09b18fef_479fc77d_0.txt |
 +---10 days \| ALC013_pass_557321c9_0.txt \|
 ANM449_pass_ec7b8135_0169951c_0.txt \| AOL145_pass_5ce9a6fb_ea26ef4a_0.txt
 \| AOO261_pass_7a496160_90dccef5_0.txt | +---14 days \|
 AJO736_pass_8dda8496_0.txt \| ALB608_pass_9cd1060c_0.txt \|
 ALT654_pass_7ec3ce48_0.txt \| ANN273_pass_4cbce703_117b6910_0.txt | +---21
 days \| ALC072_pass_4f8beeab_0.txt \| ANT028_pass_7dbff7e2_d895ac13_0.txt
 \| ANU496_pass_7a62ef46_77febc71_0.txt \|
 AOL214_pass_de7e944e_b75bcd03_0.txt | +---28 days \|
 AJU547_pass_25edcf12_0.txt \| ALB270_pass_5a306b17_0.txt \|
 ALR652_pass_48dffbe9_0.txt \| AON611_pass_eb1217db_2344207a_0.txt | +---2
 months \| ALM138_pass_22a42b73_0.txt \|
 AOL206_pass_922ab2db_04f992cf_0.txt \| AOL534_pass_ccc161da_3d5166e1_0.txt
 \| AOM261_pass_559ad00e_0ba2bf5b_0.txt | +---3 months \|
 ANG280_pass_1faa73da_0.txt \| ANO532_pass_6dfc9979_0.txt \|
 ANO756_pass_b7acb270_0.txt \| AOL584_pass_0540ecf7_ada5001a_0.txt | +---4
 months \| ANG698_pass_253d9022_0.txt \| ANP242_pass_343f75a8_0.txt \|
 ANQ483_pass_48ae7bfa_0.txt \| AOT468_pass_8e792725_1286f3c4_0.txt | +---6
 months \| ANG702_pass_54ea346b_0.txt \| ANH875_pass_0934c646_0.txt \|
 ANP446_pass_90fea4ca_0.txt \| AOV830_pass_91b38c3f_78d37fb2_0.txt |
 +---w_1day \| FAW77777_pass_barcode08_8ca636cc_e83af5d2_0.txt \|
 FAW81892_pass_barcode01_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode09_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode14_f810f4db_dc345a2f_0.txt | +---w_2days \|
 FAW77777_pass_barcode09_8ca636cc_e83af5d2_0.txt \|
 FAW81892_pass_barcode02_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode10_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode15_f810f4db_dc345a2f_0..txt | +---w_3days \|
 FAW77777_pass_barcode10_8ca636cc_e83af5d2_0.txt \|
 FAW81892_pass_barcode03_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode11_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode16_f810f4db_dc345a2f_0.txt | +---w_5days \|
 FAW77777_pass_barcode11_8ca636cc_e83af5d2_0.txt \|
 FAW81892_pass_barcode04_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode12_f810f4db_dc345a2f_0.txt \|
 FAW81892_pass_barcode17_f810f4db_dc345a2f_0.txt | +---w_8days ```
 FAW77777_pass_barcode12_8ca636cc_e83af5d2_0.txt
 FAW81892_pass_barcode07_f810f4db_dc345a2f_0.txt
 FAW81892_pass_barcode13_f810f4db_dc345a2f_0.txt
 FAW81892_pass_barcode18_f810f4db_dc345a2f_0.txt ```