cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.8239971
title: [IO Islamic 2344] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1417 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2344 here with notations and hyperlinks].</strong> 1417 Futûḥ-alḥaramain (فتوح الحرمين). A poetical description of the two holy cities, Makkah and Madînah, and of the observances during the pilgrimage, by Muḥyî Lârî, one of the pupils of learned Dawânî (who died A.H. 908 = A.D. 1502, 1503, see Haft Iḳlîm, No. 167, col. 390 in this Cat.). A. Sprenger, Catal., p. 451, ascribes this poem wrongly to Jâmî (see on the origin of this mistake Rieu ii. p. 655), and the same has been done on fol. 1<sup>a</sup> of No. 887 (1420 in this Cat.). According to the Vienna copy (G. Flügel ii. p. 122) it was composed A.H. 911 (A.D. 1505, 1506); the poet died A.H. 933 (A.D. 1526, 1527); see Haft Iḳlîm, No. 268 (col. 398 in this Cat.), and Ḥ. Khalfa iv. p. 385, No. 8922; comp. also for further details on Muḥyî and his work, Rieu ii. p. 655; W. Pertsch, Berlin Cat., pp. 260 and 261; Wiener Jahrbücher, vol. 71, Anzeigeblatt, p. 49, and Schefer, Sefer Nameh, Paris, 1881, Introd., pp. 57 and 58. It has been lithographed at Lucknow, A.H. 1292. Beginning: اى همه كس را بدرت التجا – كعبۀ دل را زتو نور وصفا The author׳s name, Muḥyî, appears on fol. 3<sup>b</sup>, l. 2. In an entry, on fol. 1<sup>a</sup>, this poem is incorrectly styled مناسك الحجّ . It is dated the 10th of Sha’bân, A.H. 1006 (A.D. 1598, March 18), at Makkah, by Imâm Ḳulî Ḳandarî (Ḳunduzî?) bin Daulatḳadam; it was bought of Sayyid Ḥusain at Mîlâpûr the 11th of Rabî’-alawwal, A.H. 1187 (A.D. 1773, June 2). College of Fort William, 1825. No. 2344, ff. 41, coll., each ll. 17; Nasta’lîḳ, ff. 1-4 supplied by a more modern hand on white paper; many leaves of the older part greatly injured, half of fol. 5 being torn away besides; illustrations on ff. 20<sup>b</sup>, 22<sup>a</sup>, 23<sup>a</sup>, 24<sup>a</sup>, 24<sup>b</sup>, 26<sup>a</sup>, 26<sup>b</sup>, 27<sup>b</sup>, 28<sup>a</sup>,31<sup>a</sup>, 32<sup>a</sup>, 34<sup>a</sup>, 36<sup>b</sup>, 39<sup>a</sup>, 40<sup>b</sup>, and 41<sup>a</sup>; size 7<sup>7/8</sup> in.by 4<sup>3/8</sup> in.
DOI: 10.17909/5bmh-yx42
title: UV and optical data for the Sunburst Ard
DOI: 10.5281/zenodo.8239977
title: [IO Islamic 208] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1418 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 208 here with notations and hyperlinks].</strong> 1418 Another copy of the same. Beginning the same as in the preceding copy; the poem concludes on fol.56<sup>b</sup>, and is followed, on ff. 57<sup>b</sup>-73<sup>b</sup>, by the same anonymous prose-treatise on the holy places of Makkah, which is noticed in No. 426 of the Bodleian Cat., beginning here: بدان ايّدك الله بنيل هذه السّعادات و الكرامات كه اين كلمۀ چند مسطور شده در بيان ذراع حرم مكّۀ معظّمه حرّمها الله تعالى عن الآفات و البليّات و مساحت مسجد الحرام كه عبارتست از حرم كعبۀ معظّمۀ مشرّفه زاد الله تعالى تعظيمًا و تشريفًا امّا قبل از شروع بدانكه الخ. Both the poem and the prose-treatise are written A.H. 1070 (A.D. 1659, 1660) by Nûr Muḥammad, the author of a کليد خواجه حافظ (see No. 1273 in this Cat.), who may be himself perhaps the compiler of the latter. No. 208, ff. 1-73, ll. 12 (2 coll. on ff. 1-56); Nasta’lîḳ; illuminated frontispiece; illustrations on ff. 24<sup>a</sup>, 24<sup>b</sup>, 27<sup>a</sup>, 27<sup>b</sup>, 31<sup>a</sup>, 32<sup>a</sup>, 33<sup>a</sup>, 33<sup>b</sup>, 38<sup>a</sup>, 39<sup>b</sup>, 42<sup>b</sup>, 48<sup>b</sup>, 51<sup>a</sup>, 53<sup>a</sup>, 53<sup>b</sup>, 55<sup>a</sup>, and 55<sup>b</sup>; fol. 54<sup>b</sup> left blank; many pages damped with wet; size, 9<sup>1/2</sup> in. by 5<sup>1/2</sup> in.
DOI: 10.5281/zenodo.8239976
title: [IO Islamic 208] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1418 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 208 here with notations and hyperlinks].</strong> 1418 Another copy of the same. Beginning the same as in the preceding copy; the poem concludes on fol.56<sup>b</sup>, and is followed, on ff. 57<sup>b</sup>-73<sup>b</sup>, by the same anonymous prose-treatise on the holy places of Makkah, which is noticed in No. 426 of the Bodleian Cat., beginning here: بدان ايّدك الله بنيل هذه السّعادات و الكرامات كه اين كلمۀ چند مسطور شده در بيان ذراع حرم مكّۀ معظّمه حرّمها الله تعالى عن الآفات و البليّات و مساحت مسجد الحرام كه عبارتست از حرم كعبۀ معظّمۀ مشرّفه زاد الله تعالى تعظيمًا و تشريفًا امّا قبل از شروع بدانكه الخ. Both the poem and the prose-treatise are written A.H. 1070 (A.D. 1659, 1660) by Nûr Muḥammad, the author of a کليد خواجه حافظ (see No. 1273 in this Cat.), who may be himself perhaps the compiler of the latter. No. 208, ff. 1-73, ll. 12 (2 coll. on ff. 1-56); Nasta’lîḳ; illuminated frontispiece; illustrations on ff. 24<sup>a</sup>, 24<sup>b</sup>, 27<sup>a</sup>, 27<sup>b</sup>, 31<sup>a</sup>, 32<sup>a</sup>, 33<sup>a</sup>, 33<sup>b</sup>, 38<sup>a</sup>, 39<sup>b</sup>, 42<sup>b</sup>, 48<sup>b</sup>, 51<sup>a</sup>, 53<sup>a</sup>, 53<sup>b</sup>, 55<sup>a</sup>, and 55<sup>b</sup>; fol. 54<sup>b</sup> left blank; many pages damped with wet; size, 9<sup>1/2</sup> in. by 5<sup>1/2</sup> in.
DOI: 10.5281/zenodo.8239981
title: [IO Islamic 2251] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1419 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2251 here with notations and hyperlinks].</strong> 1419 The same. This very clear and distinct copy is dated the 10th Jumâdâ-althânî, A.H. 1185 (A.D. 1771, Sept. 20). Beginning as in the preceding copies. College of Fort William, 1825. No. 2251, ff. 36, 2 coll., each ll. 13; distinct Nasta’lîḳ: illuminated frontispiece ; illustrations on ff. 11<sup>b</sup>, 13<sup>a</sup>, 13<sup>b</sup>, 14<sup>b</sup>, 15<sup>b</sup>, 16<sup>b</sup>, 17<sup>b</sup>, 18<sup>a</sup>, 20<sup>b</sup>, 22<sup>a</sup>, 23<sup>b</sup>, 24<sup>b</sup>, 30<sup>b</sup>, 33<sup>a</sup>, 34<sup>a</sup>, 35<sup>a</sup>, and 35<sup>b</sup>; size, 8<sup>7/8</sup> in. by 5<sup>5/8</sup> in.
DOI: 10.5281/zenodo.8239980
title: [IO Islamic 2251] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1419 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2251 here with notations and hyperlinks].</strong> 1419 The same. This very clear and distinct copy is dated the 10th Jumâdâ-althânî, A.H. 1185 (A.D. 1771, Sept. 20). Beginning as in the preceding copies. College of Fort William, 1825. No. 2251, ff. 36, 2 coll., each ll. 13; distinct Nasta’lîḳ: illuminated frontispiece ; illustrations on ff. 11<sup>b</sup>, 13<sup>a</sup>, 13<sup>b</sup>, 14<sup>b</sup>, 15<sup>b</sup>, 16<sup>b</sup>, 17<sup>b</sup>, 18<sup>a</sup>, 20<sup>b</sup>, 22<sup>a</sup>, 23<sup>b</sup>, 24<sup>b</sup>, 30<sup>b</sup>, 33<sup>a</sup>, 34<sup>a</sup>, 35<sup>a</sup>, and 35<sup>b</sup>; size, 8<sup>7/8</sup> in. by 5<sup>5/8</sup> in.
DOI: 10.6084/m9.figshare.23720949
title: Raw Data

description: Raw data
DOI: 10.6084/m9.figshare.23720949.v2
title: Raw Data

description: Raw data
DOI: 10.5281/zenodo.8239986
title: [IO Islamic 887] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1420 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 887 here with notations and hyperlinks].</strong> 1420 The same. This copy is wrongly styled, on fol. 1<sup>a</sup>, فتوح الحرمين تصنيف جامى (see No. 1417 above); but on fol. 1<sup>b</sup> the proper statement appears, كتاب فتوح الحرمين مولانا محيى . The beginning is different here from that in the preceding copies, the first bait runs thus: اى دو جهان غرقۀ آلاى تو – كون و مكان قطرۀ درياى تو (corresponding to the initial bait in the Berlin copy, and to the twenty-second verse in the preceding copy, No. 2251, fol. 2<sup>b</sup>, I. 1). The author’s name appears here, on ff. 4<sup>a</sup>, l. 12, 43<sup>a</sup>, l. 4, and 54<sup>a</sup>, l. 3. No date. No. 887, ff. 55. 2 coll., each ll. 14; clear Nasta’lîḳ; illustrations on ff. 14<sup>a</sup>, 25<sup>b</sup>, 27<sup>a</sup>, 27<sup>b</sup>, 32<sup>a</sup>, 36<sup>b</sup>, 38<sup>a</sup>, 39<sup>b</sup>, 46<sup>b</sup>, 49<sup>a</sup>, 51<sup>a</sup>, 51<sup>b</sup>, 52<sup>a</sup>, 52<sup>b</sup>, 54<sup>b</sup>, and 55<sup>a</sup>; size, 9<sup>3/4</sup> in. by 5<sup>7/8</sup> in.
DOI: 10.5281/zenodo.8239985
title: [IO Islamic 887] فتوح الحرمين

description: <strong>Futûḥ-Alḥaramain.</strong> <strong>This manuscript is now IO Islamic 1420 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 887 here with notations and hyperlinks].</strong> 1420 The same. This copy is wrongly styled, on fol. 1<sup>a</sup>, فتوح الحرمين تصنيف جامى (see No. 1417 above); but on fol. 1<sup>b</sup> the proper statement appears, كتاب فتوح الحرمين مولانا محيى . The beginning is different here from that in the preceding copies, the first bait runs thus: اى دو جهان غرقۀ آلاى تو – كون و مكان قطرۀ درياى تو (corresponding to the initial bait in the Berlin copy, and to the twenty-second verse in the preceding copy, No. 2251, fol. 2<sup>b</sup>, I. 1). The author’s name appears here, on ff. 4<sup>a</sup>, l. 12, 43<sup>a</sup>, l. 4, and 54<sup>a</sup>, l. 3. No date. No. 887, ff. 55. 2 coll., each ll. 14; clear Nasta’lîḳ; illustrations on ff. 14<sup>a</sup>, 25<sup>b</sup>, 27<sup>a</sup>, 27<sup>b</sup>, 32<sup>a</sup>, 36<sup>b</sup>, 38<sup>a</sup>, 39<sup>b</sup>, 46<sup>b</sup>, 49<sup>a</sup>, 51<sup>a</sup>, 51<sup>b</sup>, 52<sup>a</sup>, 52<sup>b</sup>, 54<sup>b</sup>, and 55<sup>a</sup>; size, 9<sup>3/4</sup> in. by 5<sup>7/8</sup> in.
DOI: 10.5281/zenodo.8239988
title: [IO Islamic 2108] نظم الدرر

description: <strong>Nażm-Aldurar</strong><strong>.</strong> <strong>This manuscript is now IO Islamic 1421 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2108 here with notations and hyperlinks].</strong> 1421 Nażm-aldurar (نظم الدرر). A didactic mathnawî, illustrating, like Sanâ’î’s Ḥadîḳah, Sa’dî’s Bûstân, and similar poems, various moral and religious maxims, interspersed with short tales, by a poet with the name of Muwâlî (see, for instance, fol. 47<sup>b</sup>, l. 10, fol. 48<sup>a</sup>, lin. penult., fol. 122<sup>a</sup>, l. 6, etc.). According to the ta’rîkh at the end, on fol. 123<sup>a</sup>, گشت فيض الهيش تأريخ, it was composed A.H. 936 (A.D. 1529, 1530); it is dedicated to Shâh Ṭahmâsp, see fol. 48<sup>b</sup>, l. 8, and fol. 122<sup>b</sup>, l. 2. The author is probably identical with Muwâlî Tûnî, who died, according to Taḳî Kâshi (A. Sprenger, Catal., p. 45, No. 649), A.H. 949 or 959 (A.D. 1542, 1543, or 1552); the same poet is mentioned in Haft Iḳlîm, No. 817 (col. 437 in this Cat.). Another Muwâlî, viz. Muwâlî Lârî, a great admirer of Ḥâfiż, is quoted Haft Iḳlîm, No. 269 (col. 398 in this Cat.); Safînah, No. 31 (col. 213 in the Bodleian Cat.), and Âtashkada, No. 693 (ib. col. 285, where he is said to have been called Khurâsânkhân); a third Muwâlî or rather Muwwâlî, a Turkmân under Shâh ‘Abbâs II, is quoted in W. Pertsch, Turkish Cat., No. 168, and Berlin Cat., p. 701 (No. 41). This copy, the only one hitherto known, is unfortunately defective both in the beginning and in the middle. It opens abruptly with this bait: اين نگارندۀ سپید و سیاه – هر دو عالم بهستئ تو گواه (there seems to be <em>one</em> page missing). A lacuna of two leaves, besides, after fol. 90. The title of the work appears on fol. 47<sup>b</sup>, l. 11: خواستم نام اين كتاب از غيب گشت نظم الدرر خطاب ازغيب Dated by Ćand Muḥammad bin Jamâl Muḥammad Nausârî, the 22nd of Jumâdâ-alawwal, A.H. 1030 (?) (A.D. 1621, April 14), comp. No, 1297 in this Cat. No. 2108, ff. 46-123, 2 coll., each ll. 13; Nasta’lîḳ; size,7¼ in. by 4¾ in.
DOI: 10.5281/zenodo.8239987
title: [IO Islamic 2108] نظم الدرر

description: <strong>Nażm-Aldurar</strong><strong>.</strong> <strong>This manuscript is now IO Islamic 1421 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2108 here with notations and hyperlinks].</strong> 1421 Nażm-aldurar (نظم الدرر). A didactic mathnawî, illustrating, like Sanâ’î’s Ḥadîḳah, Sa’dî’s Bûstân, and similar poems, various moral and religious maxims, interspersed with short tales, by a poet with the name of Muwâlî (see, for instance, fol. 47<sup>b</sup>, l. 10, fol. 48<sup>a</sup>, lin. penult., fol. 122<sup>a</sup>, l. 6, etc.). According to the ta’rîkh at the end, on fol. 123<sup>a</sup>, گشت فيض الهيش تأريخ, it was composed A.H. 936 (A.D. 1529, 1530); it is dedicated to Shâh Ṭahmâsp, see fol. 48<sup>b</sup>, l. 8, and fol. 122<sup>b</sup>, l. 2. The author is probably identical with Muwâlî Tûnî, who died, according to Taḳî Kâshi (A. Sprenger, Catal., p. 45, No. 649), A.H. 949 or 959 (A.D. 1542, 1543, or 1552); the same poet is mentioned in Haft Iḳlîm, No. 817 (col. 437 in this Cat.). Another Muwâlî, viz. Muwâlî Lârî, a great admirer of Ḥâfiż, is quoted Haft Iḳlîm, No. 269 (col. 398 in this Cat.); Safînah, No. 31 (col. 213 in the Bodleian Cat.), and Âtashkada, No. 693 (ib. col. 285, where he is said to have been called Khurâsânkhân); a third Muwâlî or rather Muwwâlî, a Turkmân under Shâh ‘Abbâs II, is quoted in W. Pertsch, Turkish Cat., No. 168, and Berlin Cat., p. 701 (No. 41). This copy, the only one hitherto known, is unfortunately defective both in the beginning and in the middle. It opens abruptly with this bait: اين نگارندۀ سپید و سیاه – هر دو عالم بهستئ تو گواه (there seems to be <em>one</em> page missing). A lacuna of two leaves, besides, after fol. 90. The title of the work appears on fol. 47<sup>b</sup>, l. 11: خواستم نام اين كتاب از غيب گشت نظم الدرر خطاب ازغيب Dated by Ćand Muḥammad bin Jamâl Muḥammad Nausârî, the 22nd of Jumâdâ-alawwal, A.H. 1030 (?) (A.D. 1621, April 14), comp. No, 1297 in this Cat. No. 2108, ff. 46-123, 2 coll., each ll. 13; Nasta’lîḳ; size,7¼ in. by 4¾ in.
DOI: 10.5281/zenodo.8239990
title: [IO Islamic 2108] مرآة الحقائق

description: <strong>Mirât-Alḥaḳâ’iḳ.</strong> <strong>This manuscript is now IO Islamic 1422 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2108 here with notations and hyperlinks].</strong> 1422 Mirât-alḥaḳâ’iḳ (مرآة الحقائق). The mirror of truth, another short didactic poem in the form of a ḳaṣîdah, by the same Muwâlî, beginning: سخن را ابتدا كردم بنام حىّ منّانش عليم عالمش ميدانم و قيّوم رحمانش The title occurs in the last bait but one, on fol. 128<sup>a</sup>: بمرآت الحقائق شد مسمّى اين كلام من خداوندا بكن نامى زفرط و فضل احسانش Dated by the same transcriber, on the same day in the same year, as the preceding نظم الدرر, and concluded by a few baits from the copyist’s pen. No. 2108, ff. 123<sup>b</sup>-128<sup>a</sup>, ll. 13; Nasta’lîḳ; size, 7¼ in. by 4¾ in.
DOI: 10.5281/zenodo.8239989
title: [IO Islamic 2108] مرآة الحقائق

description: <strong>Mirât-Alḥaḳâ’iḳ.</strong> <strong>This manuscript is now IO Islamic 1422 in the India Office collections.</strong> <strong>[metadata:</strong> <strong>Hermann Ethé</strong><strong><em>, </em></strong><strong><em>Catalogue of Persian Manuscripts in the Library of the India Office,</em></strong> <strong>2 vols. (Oxford: India Office, 1903): volume 1,</strong> <strong>number 2108 here with notations and hyperlinks].</strong> 1422 Mirât-alḥaḳâ’iḳ (مرآة الحقائق). The mirror of truth, another short didactic poem in the form of a ḳaṣîdah, by the same Muwâlî, beginning: سخن را ابتدا كردم بنام حىّ منّانش عليم عالمش ميدانم و قيّوم رحمانش The title occurs in the last bait but one, on fol. 128<sup>a</sup>: بمرآت الحقائق شد مسمّى اين كلام من خداوندا بكن نامى زفرط و فضل احسانش Dated by the same transcriber, on the same day in the same year, as the preceding نظم الدرر, and concluded by a few baits from the copyist’s pen. No. 2108, ff. 123<sup>b</sup>-128<sup>a</sup>, ll. 13; Nasta’lîḳ; size, 7¼ in. by 4¾ in.
DOI: 10.6084/m9.figshare.23935151
title: ZNF143 inhibits hepatocyte mitophagy and promotes non-alcoholic fatty liver disease by targeting increased lncRNA NEAT1 expression to activate ROCK2 pathway

description: Background: Nonalcoholic fatty liver disease (NAFLD) is the most common hepatic disorders worldwide. The mitophagy is suggested to be repressed in NAFLD, but the mechanism remains to be elucidated. Methods: NAFLD cell and mouse models were established by treating with free fatty acid (FFA) and feeding a high fat diet (HFD), respectively. QRT-PCR, Western blotting, or IHC measured the expression of ZNF143, lncRNA NEAT1, ROCK2, and lipid formation/mitophagy-related proteins. Cell viability and mitophagy were evaluated by MTT and immunofluorescence. The chloroform-methanol extraction method measured triglyceride and total cholesterol levels. ELISA detected ALT and AST levels. The interactions among ZNF143, lncRNA NEAT1 and SND1 were analysed by ChIP, dual-luciferase reporter, pull-down, and RIP. The lipid droplets were determined by Oil-red O and HE staining. Results: ZNF143 and lncRNA NEAT1 were upregulated in hepatic cells treated with FFA (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). Knockdown of ZNF143 or lncRNA NEAT1 inhibited lipid droplets formation, while promoting mitophagy (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). ZNF143 promoted lncRNA NEAT1 transcriptional expression through binding to its promoter. LncRNA NEAT1 increased ROCK2 mRNA stability by targeting SND1. LncRNA NEAT1 or ROCK2 overexpression reversed the effect of ZNF143 or lncRNA NEAT1 knockdown on hepatic steatosis and mitophagy (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). ZNF143 or lncRNA NEAT1 knockdown inhibited HFD-induced steatosis and promoted mitophagy <i>in vivo</i> (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). Conclusion: The upregulation of lncRNA NEAT1 caused by ZNF143 promoted NAFLD through inhibiting mitophagy via activating ROCK2 pathway by targeting SND1, providing potential targets for NAFLD therapy.
DOI: 10.6084/m9.figshare.23935151.v1
title: ZNF143 inhibits hepatocyte mitophagy and promotes non-alcoholic fatty liver disease by targeting increased lncRNA NEAT1 expression to activate ROCK2 pathway

description: Background: Nonalcoholic fatty liver disease (NAFLD) is the most common hepatic disorders worldwide. The mitophagy is suggested to be repressed in NAFLD, but the mechanism remains to be elucidated. Methods: NAFLD cell and mouse models were established by treating with free fatty acid (FFA) and feeding a high fat diet (HFD), respectively. QRT-PCR, Western blotting, or IHC measured the expression of ZNF143, lncRNA NEAT1, ROCK2, and lipid formation/mitophagy-related proteins. Cell viability and mitophagy were evaluated by MTT and immunofluorescence. The chloroform-methanol extraction method measured triglyceride and total cholesterol levels. ELISA detected ALT and AST levels. The interactions among ZNF143, lncRNA NEAT1 and SND1 were analysed by ChIP, dual-luciferase reporter, pull-down, and RIP. The lipid droplets were determined by Oil-red O and HE staining. Results: ZNF143 and lncRNA NEAT1 were upregulated in hepatic cells treated with FFA (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). Knockdown of ZNF143 or lncRNA NEAT1 inhibited lipid droplets formation, while promoting mitophagy (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). ZNF143 promoted lncRNA NEAT1 transcriptional expression through binding to its promoter. LncRNA NEAT1 increased ROCK2 mRNA stability by targeting SND1. LncRNA NEAT1 or ROCK2 overexpression reversed the effect of ZNF143 or lncRNA NEAT1 knockdown on hepatic steatosis and mitophagy (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). ZNF143 or lncRNA NEAT1 knockdown inhibited HFD-induced steatosis and promoted mitophagy <i>in vivo</i> (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001). Conclusion: The upregulation of lncRNA NEAT1 caused by ZNF143 promoted NAFLD through inhibiting mitophagy via activating ROCK2 pathway by targeting SND1, providing potential targets for NAFLD therapy.