cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.19615779
title: Scripts and data on Wnt signalling, cell fate determination and anteroposterior polarity of the skate gill arch skeleton

description: <strong>Scripts:</strong> Scripts S.3.1–S.3.4 detail bash script containing the code used to assemble the Leucoraja erinacea GAER transcriptome. Includes: - Script S.3.1: Initial quality check on raw data. - Script S.3.2: Recleaning of data to include only 149-150bp reads. - Script S.3.3: Normalisation of data pre-assembly. - Script S.3.4: Trinity assembly. <br> Scripts S.3.5–S.3.7 detail bash script containing the code used to check my assembled transcriptome. Includes: - Script S.3.5: Assessing RNA-Seq read representation. - Script S.3.6: Assessing representation of full length reconstructed protein coding genes by searching the assembled transcripts against Uniprot. Includes counting full length transcripts and examining the percent of the target being aligned to by the best matching Trinity transcript. - Script S.3.7: Calculating the transcript abundance estimation. <br> Scripts S.3.8–S.3.10 contain the code used for differential expression analysis and analysing genes of interest. Includes: - Script S.3.8: Bash script calculating the transcript abundance estimation to generate a salmon gene counts matrix file removing two libraries. - Script S.3.9: Bash script for extracting fasta sequences of highest expressed isoforms. - Script S.3.10.Rmd: R file detailing differential gene expression analysis and generation of volcano plots. <br> <strong>Tables:</strong> - Table S.3.1: <em>Leucoraja erinacea</em> genes differentially expressed in GAER vs control tissue manually annotated with gene names. - Table S.3.2: <em>Leucoraja erinacea</em> genes differentially expressed in GAER vs control tissue annotated with colour labelling for volcano plot. - Table S.3.3: Raw data of Salmon gene counts matrix. - Table S.3.4: Salmon gene expression matrix. - Table S.3.5: Assembly statistics. <strong>Figures:</strong> - Fig. S.3.1: Graphs showing biological coefficient of variation, mean difference plot and multidimentional scaling plots pre and post-batch effect.
DOI: 10.6084/m9.figshare.19615779.v1
title: Scripts and data on Wnt signalling, cell fate determination and anteroposterior polarity of the skate gill arch skeleton

description: <strong>Scripts:</strong> Scripts A.3.1–A.3.4 detail bash script containing the code used to assemble the Leucoraja erinacea GAER transcriptome. Includes: - Script A.3.1: Initial quality check on raw data. - Script A.3.2: Recleaning of data to include only 149-150bp reads. - Script A.3.3: Normalisation of data pre-assembly. - Script A.3.4: Trinity assembly. <br> Scripts A.3.5–A.3.7 detail bash script containing the code used to check my assembled transcriptome. Includes: - Script A.3.5: Assessing RNA-Seq read representation. - Script A.3.6: Assessing representation of full length reconstructed protein coding genes by searching the assembled transcripts against Uniprot. Includes counting full length transcripts and examining the percent of the target being aligned to by the best matching Trinity transcript. - Script A.3.7: Calculating the transcript abundance estimation. <br> Scripts A.3.8–A.3.10 contain the code used for differential expression analysis and analysing genes of interest. Includes: - Script A.3.8: Bash script calculating the transcript abundance estimation to generate a salmon gene counts matrix file removing two libraries. - Script A.3.9: Bash script for extracting fasta sequences of highest expressed isoforms. - Script A.3.10.Rmd: R file detailing differential gene expression analysis and generation of volcano plots. <br> <strong>Tables:</strong> - Table A.3.1: <em>Leucoraja erinacea</em> genes differentially expressed in GAER vs control tissue manually annotated with gene names. - Table A.3.2: <em>Leucoraja erinacea</em> genes differentially expressed in GAER vs control tissue annotated with colour labelling for volcano plot. - Table A.3.3: Raw data of Salmon gene counts matrix. - Table A.3.4: Salmon gene expression matrix. <br> <strong>Figures:</strong> - Fig. A.3.1: Graphs showing biological coefficient of variation, mean difference plot and multidimentional scaling plots pre and post-batch effect.
DOI: 10.35077/add-met
title: Confocal imaging of developing fly brain labeling CG14322+ PNs and 91G04+ PNs: Fly 6

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DL1/DA3 PNs (CG14322+) and DC2 PNs (91G04+) with SNAP and Halo, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly6_brain_1 and Fly6_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively.
description: Fly6_brain_1 and Fly6_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling CG14322+ PNs and 91G04+ PNs: Fly 6', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_02_Fly6', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'GH146-FLP/UAS-FRT10-stop-FRT10-3xHalo7-CAAX; 91G04-GAL4/CG14322-T2A-QF2 QUAS-FRT-stop-FRT-mCD8-myr-4xSNAPf', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.5281/zenodo.7895889
title: Data of crop yield derived from H2020 Diverfarming project

description: Crop yield data of a mandarin crop (main crop) and different secondary crops (alley crops) cultivated in different cropping systems (monocrop and diversification) with different treatments (control irrigation and regulated deficit irrigation)
DOI: 10.5281/zenodo.7895888
title: Data of crop yield derived from H2020 Diverfarming project

description: Crop yield data of a mandarin crop (main crop) and different secondary crops (alley crops) cultivated in different cropping systems (monocrop and diversification) with different treatments (control irrigation and regulated deficit irrigation)
DOI: 10.35077/add-mix
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 1

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly1_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly1_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 1', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly1', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-mob
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 2

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly2_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly2_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 2', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly2', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-moo
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 3

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly3_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly3_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 3', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly3', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-mop
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 4

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly4_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly4_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 4', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly4', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-mud
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 5

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly5_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly5_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 5', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly5', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-mug
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 6

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly6_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly6_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 6', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly6', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.21227/p5dg-vr48
title: P57H22

description: P57H22
DOI: 10.21227/2atz-3s23
title: P57H22

description: P57H22
DOI: 10.35077/add-nab
title: Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 7

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly7_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain.
description: Fly7_brain_1 show antennal lobe regions in both left and right hemispheres of the same brain. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling Mz19+ acj6+ PNs and Mz19+ acj6- PNs: Fly 7', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_03_Fly7', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'acj6-T2A-FLP/+; Mz19-GAL4; UAS-FRT-myr-4xSNAPf-FRT-3xHalo7-CAAX/+', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-nap
title: Confocal imaging of developing fly brain labeling tsh+ PNs and CG14322+ PNs: Fly 1

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DL1/DA3 PNs (CG14322+) and DA1/DL3 PNs (tsh+) with SNAP and Halo, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly1_brain_1 and Fly1_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively.
description: Fly1_brain_1 and Fly1_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling tsh+ PNs and CG14322+ PNs: Fly 1', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_04_Fly1', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'GH146-FLP/tsh-GAL4; UAS-FRT10-stop-FRT10-3xHalo7-CAAX/CG14322-T2A-QF2 QUAS-FRT-stop-FRT-mCD8-myr-4xSNAPf', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-net
title: Confocal imaging of developing fly brain labeling tsh+ PNs and CG14322+ PNs: Fly 2

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DL1/DA3 PNs (CG14322+) and DA1/DL3 PNs (tsh+) with SNAP and Halo, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly2_brain_1 and Fly2_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively.
description: Fly2_brain_1 and Fly2_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling tsh+ PNs and CG14322+ PNs: Fly 2', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_04_Fly2', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'GH146-FLP/tsh-GAL4; UAS-FRT10-stop-FRT10-3xHalo7-CAAX/CG14322-T2A-QF2 QUAS-FRT-stop-FRT-mCD8-myr-4xSNAPf', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-new
title: Confocal imaging of developing fly brain labeling tsh+ PNs and CG14322+ PNs: Fly 3

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DL1/DA3 PNs (CG14322+) and DA1/DL3 PNs (tsh+) with SNAP and Halo, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly3_brain_1 and Fly3_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively.
description: Fly3_brain_1 and Fly3_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling tsh+ PNs and CG14322+ PNs: Fly 3', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_04_Fly3', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'GH146-FLP/tsh-GAL4; UAS-FRT10-stop-FRT10-3xHalo7-CAAX/CG14322-T2A-QF2 QUAS-FRT-stop-FRT-mCD8-myr-4xSNAPf', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'
DOI: 10.35077/add-nil
title: Confocal imaging of developing fly brain labeling lov+ PNs and 91G04+ PNs: Fly 1

description: This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled lov+ PNs and DC2 PNs (91G04+) with GFP and Halo, respectively and examined their dendrite targeting in the developing fly brain.
description: Fly1_brain_1 and Fly1_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively.
description: Fly1_brain_1 and Fly1_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively. DATASET METADATA: title:'Confocal imaging of developing fly brain labeling lov+ PNs and 91G04+ PNs: Fly 1', GeneralModality:'cell morphology', Technique:'other', Other:'chemical labeling, immunostaining' SPECIMEN METADATA: LocalID:'Dataset_05_Fly1', Species:'Fruit Fly', NCBITaxonomy:'NCBI:txid7227', Age:'12', Ageunit:'hours after puparium formation', Sex:'Unknown', Genotype:'GH146-FLP QUAS-FRT-stop-FRT-mCD8-GFP/lov-T2A-QF2; 91G04-GAL4/UAS-FRT10-stop-FRT10-3xHalo7-CAAX', OrganName:'Brain', SampleLocalID:'NA', Locations:'Antennal lobe' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'ZEISS LSM 780; LSM 900 with Airyscan 2' IMAGE METADATA: xAxis:'right-to-left', yAxis:'superior-to-inferior', zAxis:'anterior-to-posterior', Number:'1,2,red,green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', stepSizeX:'0.25', stepSizeY:'0.25'