cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5792019
title: Singerocomus atlanticus sp. nov., and a first record of Singerocomus rubriflavus (Boletaceae, Boletales) for Brazil

description: ABSTRACT Ongoing surveys of macrofungi in the Brazilian Atlantic Forest ecoregion continue to uncover a diverse assemblage of ectomycorrhizal fungi. A new species of Boletaceae, Singerocomus atlanticus sp. nov., is described. Singerocomus rubriflavus, previously known only from Guyana, is recorded for the first time from the Brazilian Atlantic Forest. Phylogenetic data, macro- and microscopic illustrations, and comments are presented for each species.
DOI: 10.6084/m9.figshare.5792019.v1
title: Singerocomus atlanticus sp. nov., and a first record of Singerocomus rubriflavus (Boletaceae, Boletales) for Brazil

description: ABSTRACT Ongoing surveys of macrofungi in the Brazilian Atlantic Forest ecoregion continue to uncover a diverse assemblage of ectomycorrhizal fungi. A new species of Boletaceae, Singerocomus atlanticus sp. nov., is described. Singerocomus rubriflavus, previously known only from Guyana, is recorded for the first time from the Brazilian Atlantic Forest. Phylogenetic data, macro- and microscopic illustrations, and comments are presented for each species.
DOI: 10.6084/m9.figshare.5792016
title: Planting density and training in grafted indeterminate tomato plants grown under plastic cover

description: ABSTRACT Grafting in tomato is a regular production practice aimed to control vascular diseases. Even though grafted plants are more expensive, when a vigorous rootstock is used the plants can be trained using multiple leading shoots, reducing the number of plants per hectare. Seeking to reduce direct labor cost and the cost of the plants, this experiment tested 29,000, 26,000, 23,000 and 20,000 leading shoots/ha, training the plants with either 3 or 4 leading shoots each. Plants were grown in a polyethylene-covered greenhouse, transplanting at the end of January; the harvest was done from May until August, quantifying yield, fruit size and leaf area, analyzing the data with quadratic regression. Yield increased from 81 to 103 t/ha when comparing the lowest and the highest density of shoots/ha, but the difference between the training systems was only 4 t/ha. When the two extremes of the density were compared (29,000 vs 20,000 shoots/ha), leaf area increase by 2%, the equatorial fruit diameter increased from 7.4 to 7.8 cm, and the weight increased from 178 to 199 g/fruit. The training system showed difference of 0.1 cm in fruit diameter, while the leaf area and fruit weight were not affected. While the lowest density of shoots per hectare had a 30% reduction in direct labor cost, the largest net profit margin was observed when using 3 shoots/plant and 29,000 shoots/ha.
DOI: 10.6084/m9.figshare.5792016.v1
title: Planting density and training in grafted indeterminate tomato plants grown under plastic cover

description: ABSTRACT Grafting in tomato is a regular production practice aimed to control vascular diseases. Even though grafted plants are more expensive, when a vigorous rootstock is used the plants can be trained using multiple leading shoots, reducing the number of plants per hectare. Seeking to reduce direct labor cost and the cost of the plants, this experiment tested 29,000, 26,000, 23,000 and 20,000 leading shoots/ha, training the plants with either 3 or 4 leading shoots each. Plants were grown in a polyethylene-covered greenhouse, transplanting at the end of January; the harvest was done from May until August, quantifying yield, fruit size and leaf area, analyzing the data with quadratic regression. Yield increased from 81 to 103 t/ha when comparing the lowest and the highest density of shoots/ha, but the difference between the training systems was only 4 t/ha. When the two extremes of the density were compared (29,000 vs 20,000 shoots/ha), leaf area increase by 2%, the equatorial fruit diameter increased from 7.4 to 7.8 cm, and the weight increased from 178 to 199 g/fruit. The training system showed difference of 0.1 cm in fruit diameter, while the leaf area and fruit weight were not affected. While the lowest density of shoots per hectare had a 30% reduction in direct labor cost, the largest net profit margin was observed when using 3 shoots/plant and 29,000 shoots/ha.
DOI: 10.6084/m9.figshare.5792013
title: Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L.

description: ABSTRACT Jatropha curcas is a plant that accumulates high-quality oil in its seeds, which is capable of being transformed into liquid biofuel. However, several aspects of its flowering are still unknown, which is a key step in the production of fruits, seeds, and oil. Flowering is a complex process that is regulated by various factors at the molecular level, including microRNAs (miRNAs). There is evidence suggesting that the miRNAs of the miR156 and miR172 families play a key role in the flowering transition of plants. For this reason, the dynamics of miR156 and miR172 were studied during a production cycle of J. curcas. Our results reveal that J. curcas has a mechanism for the expression of these miRNAs that differs from that reported for the vast majority of angiosperms, since the expression of both families of miRNAs was positively correlated with the phenological state of J. curcas. We discuss the implications of these findings and how the regulatory mechanisms of miR156 and miR172 differ from what has been reported thus far.
DOI: 10.6084/m9.figshare.5792013.v1
title: Dynamics of miR156 and miR172 involved in the flowering of Jatropha curcas L.

description: ABSTRACT Jatropha curcas is a plant that accumulates high-quality oil in its seeds, which is capable of being transformed into liquid biofuel. However, several aspects of its flowering are still unknown, which is a key step in the production of fruits, seeds, and oil. Flowering is a complex process that is regulated by various factors at the molecular level, including microRNAs (miRNAs). There is evidence suggesting that the miRNAs of the miR156 and miR172 families play a key role in the flowering transition of plants. For this reason, the dynamics of miR156 and miR172 were studied during a production cycle of J. curcas. Our results reveal that J. curcas has a mechanism for the expression of these miRNAs that differs from that reported for the vast majority of angiosperms, since the expression of both families of miRNAs was positively correlated with the phenological state of J. curcas. We discuss the implications of these findings and how the regulatory mechanisms of miR156 and miR172 differ from what has been reported thus far.
DOI: 10.6084/m9.figshare.5792010
title: Families settlers in border areas of the captaincy of São Paulo during the second half of the 18th century

description: Abstract After assuming the Government of the captaincy of São Paulo in 1765, Morgado de Mateus established an expansion process of the settlement. The crisis of the colonial economy and conflicts with the Castilians demanded the intervention of the Crown, seeking to deploy new economic activities and preparing the defense of the territory. When creating towns and villages, the Governor would seek bringing together individuals classified as unruly and would send them, sometimes by force, to be the pioneers in border areas. Many of these settlers were indigenous people, considered as vagabonds, who should be submitted to the new model of social organization proposed by the Crown. This effort can be accessed through inhabitants lists of three settlement initiatives: São Luiz do Paraitinga, Piracicaba and the Caminho de Goiás. The profile analysis of the individuals installed in these new villages allows for a better understanding of the organizational strategies of the colonial population desired by the Crown.
DOI: 10.6084/m9.figshare.5792010.v1
title: Families settlers in border areas of the captaincy of São Paulo during the second half of the 18th century

description: Abstract After assuming the Government of the captaincy of São Paulo in 1765, Morgado de Mateus established an expansion process of the settlement. The crisis of the colonial economy and conflicts with the Castilians demanded the intervention of the Crown, seeking to deploy new economic activities and preparing the defense of the territory. When creating towns and villages, the Governor would seek bringing together individuals classified as unruly and would send them, sometimes by force, to be the pioneers in border areas. Many of these settlers were indigenous people, considered as vagabonds, who should be submitted to the new model of social organization proposed by the Crown. This effort can be accessed through inhabitants lists of three settlement initiatives: São Luiz do Paraitinga, Piracicaba and the Caminho de Goiás. The profile analysis of the individuals installed in these new villages allows for a better understanding of the organizational strategies of the colonial population desired by the Crown.
DOI: 10.6084/m9.figshare.5792007
title: Estimation methods and correction factors for body weight in Mangalarga Marchador horses

description: ABSTRACT The objective was to evaluate the accuracy of six body weight (BW) estimating methods in Mangalarga Marchador horses (MM) (n = 318): method A - tape placements at three different positions around the thoracic girth; B - Crevat and Quetelec's formula; C - Hall's formula; D - Hintz and Griffiths’ table; E - Santos’ table; and F - Cintra's formula. For additional analyses, gender, age, and gestational stage were considered. Estimated average BW was compared to the actual scale weight by the paired T test, mean predicted error, and determination coefficient. In the general population, methods A (position 3), B, and C estimated BW that were different from that of the scale. Method A, at positions 1 and 2, was more accurate in predicting the scale weight results compared with all other methods. For pregnant mares, the tape in positions 1 and 2 in method A did not differ from those of the scale. Method A in positions 1 and 2 and the table (method E) may be used to estimate the BW of males and females of different ages and/or gestational stages. To use Methods B and C, correction factors are necessary to precisely estimate the body weights in this breed.
DOI: 10.6084/m9.figshare.5792007.v1
title: Estimation methods and correction factors for body weight in Mangalarga Marchador horses

description: ABSTRACT The objective was to evaluate the accuracy of six body weight (BW) estimating methods in Mangalarga Marchador horses (MM) (n = 318): method A - tape placements at three different positions around the thoracic girth; B - Crevat and Quetelec's formula; C - Hall's formula; D - Hintz and Griffiths’ table; E - Santos’ table; and F - Cintra's formula. For additional analyses, gender, age, and gestational stage were considered. Estimated average BW was compared to the actual scale weight by the paired T test, mean predicted error, and determination coefficient. In the general population, methods A (position 3), B, and C estimated BW that were different from that of the scale. Method A, at positions 1 and 2, was more accurate in predicting the scale weight results compared with all other methods. For pregnant mares, the tape in positions 1 and 2 in method A did not differ from those of the scale. Method A in positions 1 and 2 and the table (method E) may be used to estimate the BW of males and females of different ages and/or gestational stages. To use Methods B and C, correction factors are necessary to precisely estimate the body weights in this breed.
DOI: 10.6084/m9.figshare.5792004
title: Therapeutic drug monitoring of benznidazole and nifurtimox: a systematic review and quality assessment of published clinical practice guidelines

description: Abstract The pharmacological management of adults with chronic-phase Chagas disease is challenging despite it being the recent focus of extensive research. One of the challenges in the current clinical practice guidelines (CPGs) landscape is the existence of non-evidence-based recommendations for the use of laboratory tests in treatment monitoring. This study aimed to systematically assess the quality and consistency of recommendations of CPGs on the pharmacological management of adults with chronic-phase Chagas disease. Systematic literature searches were conducted in MEDLINE, EMBASE, SciELO and Google to identify all published CPGs relevant to the pharmacological management of Chagas disease, between January 2010 and March 2016. Three independent reviewers assessed the quality of each CPG using the Appraisal of Guidelines Research and Evaluation (AGREE) II instrument. A total of five CPGs were included and the overall quality of the guidelines for therapeutic drug monitoring of Chagas disease was moderate-to-low. There was considerable variation in the quality of the CPGs across the AGREE II domains. The domains of scope/purpose, stakeholder involvement, and clarity of presentation were rated well, and the domains of applicability and editorial independence received poor ratings. This review showed that the methodological quality of CPGs for Chagas disease was generally inappropriate, and there was no explicit link between the best available evidence and current recommendations.
DOI: 10.6084/m9.figshare.5792004.v1
title: Therapeutic drug monitoring of benznidazole and nifurtimox: a systematic review and quality assessment of published clinical practice guidelines

description: Abstract The pharmacological management of adults with chronic-phase Chagas disease is challenging despite it being the recent focus of extensive research. One of the challenges in the current clinical practice guidelines (CPGs) landscape is the existence of non-evidence-based recommendations for the use of laboratory tests in treatment monitoring. This study aimed to systematically assess the quality and consistency of recommendations of CPGs on the pharmacological management of adults with chronic-phase Chagas disease. Systematic literature searches were conducted in MEDLINE, EMBASE, SciELO and Google to identify all published CPGs relevant to the pharmacological management of Chagas disease, between January 2010 and March 2016. Three independent reviewers assessed the quality of each CPG using the Appraisal of Guidelines Research and Evaluation (AGREE) II instrument. A total of five CPGs were included and the overall quality of the guidelines for therapeutic drug monitoring of Chagas disease was moderate-to-low. There was considerable variation in the quality of the CPGs across the AGREE II domains. The domains of scope/purpose, stakeholder involvement, and clarity of presentation were rated well, and the domains of applicability and editorial independence received poor ratings. This review showed that the methodological quality of CPGs for Chagas disease was generally inappropriate, and there was no explicit link between the best available evidence and current recommendations.
DOI: 10.6084/m9.figshare.5792001
title: At the Core of Africanization: Black and Colored People in Population Maps of Colonial Maranhão, Brazil (1798-1821)

description: Abstract The study analyzes the demographic boom of African slave populations in the plantation areas from the Mapas de População of Maranhão. In particular, we focus on the parish of Rosário do Itapecuru using other sources - post-mortem inventories and parish baptismal records. The maps from 1798 and 1821 made it possible to evidence the gender, age, ethnicity and legal status of the resident population as well as show the importance of slave population in cotton and rice crop areas, reaching almost 80% of residents. These populations, in turn, presented certain peculiarities in relation to other plantation areas in the State of Brazil, such as the sex ratio marked by the almost parity between men and women.
DOI: 10.6084/m9.figshare.5792001.v1
title: At the Core of Africanization: Black and Colored People in Population Maps of Colonial Maranhão, Brazil (1798-1821)

description: Abstract The study analyzes the demographic boom of African slave populations in the plantation areas from the Mapas de População of Maranhão. In particular, we focus on the parish of Rosário do Itapecuru using other sources - post-mortem inventories and parish baptismal records. The maps from 1798 and 1821 made it possible to evidence the gender, age, ethnicity and legal status of the resident population as well as show the importance of slave population in cotton and rice crop areas, reaching almost 80% of residents. These populations, in turn, presented certain peculiarities in relation to other plantation areas in the State of Brazil, such as the sex ratio marked by the almost parity between men and women.
DOI: 10.6084/m9.figshare.5791992
title: Cyrtopodium paludicolum germination with two Tulasnella isolates

description: ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully isolated from Cyrtopodium paludicolum and identified as Tulasnella sp. Treatments with both fungi reached a higher germination percentage than under asymbiotic conditions, indicating no specificity in the relationship between Cyrtopodium paludicolum and the fungi. The results presented have the potential to advance research into the propagation and conservation of C. paludicolum, a native of the Cerrado biome.
DOI: 10.6084/m9.figshare.5791992.v1
title: Cyrtopodium paludicolum germination with two Tulasnella isolates

description: ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully isolated from Cyrtopodium paludicolum and identified as Tulasnella sp. Treatments with both fungi reached a higher germination percentage than under asymbiotic conditions, indicating no specificity in the relationship between Cyrtopodium paludicolum and the fungi. The results presented have the potential to advance research into the propagation and conservation of C. paludicolum, a native of the Cerrado biome.
DOI: 10.6084/m9.figshare.5791989
title: Evidence of genetic differentiation and karyotype evolution of the sedges Cyperus ligularis L. and C. odoratus L. (Cyperaceae)

description: ABSTRACT The taxonomy of Cyperaceae is complex, with genera like Cyperus harboring species complexes. We analyzed the genetic similarity between Cyperus ligularis L. and C. odoratus L. based on DNA fingerprinting and cytogenetics. Significative genetic differentiation (G ST = 0.363) and low gene flow (N m = 0.877) indicated a clear genetic distinction between the two species. Moreover, the clustering analysis showed two distinct genetic groups, suggesting a lack of evidence for hybridization. The phenogram revealed two different lineages, and although all individuals of C. odoratus were collected from plots close to each other, they possessed greater genetic diversity than that observed among individuals of C. ligularis, which were sampled over a wider geographic range. Variation in chromosome number within the two species exhibited the opposite pattern, indicating greater karyotype stability in C. odoratus with 2n = 72 and 2n = 76, while the diploid number for C. ligularis varied from 2n = 66 to 88. The lower genetic variation in C. ligularis may be a result of the founder effect associated with seed dispersion and clonal reproduction. Field observations and analysis of reproductive biology should enrich the understanding of the genetic structure of the investigated populations and their role in successional processes.
DOI: 10.6084/m9.figshare.5791989.v1
title: Evidence of genetic differentiation and karyotype evolution of the sedges Cyperus ligularis L. and C. odoratus L. (Cyperaceae)

description: ABSTRACT The taxonomy of Cyperaceae is complex, with genera like Cyperus harboring species complexes. We analyzed the genetic similarity between Cyperus ligularis L. and C. odoratus L. based on DNA fingerprinting and cytogenetics. Significative genetic differentiation (G ST = 0.363) and low gene flow (N m = 0.877) indicated a clear genetic distinction between the two species. Moreover, the clustering analysis showed two distinct genetic groups, suggesting a lack of evidence for hybridization. The phenogram revealed two different lineages, and although all individuals of C. odoratus were collected from plots close to each other, they possessed greater genetic diversity than that observed among individuals of C. ligularis, which were sampled over a wider geographic range. Variation in chromosome number within the two species exhibited the opposite pattern, indicating greater karyotype stability in C. odoratus with 2n = 72 and 2n = 76, while the diploid number for C. ligularis varied from 2n = 66 to 88. The lower genetic variation in C. ligularis may be a result of the founder effect associated with seed dispersion and clonal reproduction. Field observations and analysis of reproductive biology should enrich the understanding of the genetic structure of the investigated populations and their role in successional processes.
DOI: 10.6084/m9.figshare.5791986
title: Hydrothermal treatment minimizes the effects of refrigeration in okra fruits

description: ABSTRACT This study evaluated the involvement of peroxidase and polyphenoloxidase on the enzymatic browning of okra and the role of catalase in the appearance of chilling injury in cultivars Amarelinho and Mammoth Spineless treated with hot water. Okra fruits from two cultivars were immersed in water at 40°C, during 15 and 30 minutes. The control treatment was not immersed, and all fruits were stored at 5°C. Visual analysis of fruits was carried out and total chlorophyll content and total phenolic compounds were determined. The activity of the enzymes catalase, peroxidase and polyphenoloxidase was analyzed. In all treatments, the cultivar Mammoth Spineless showed higher values of chlorophyll content and soluble phenolic compounds compared to cultivar Amarelinho. For both cultivars, catalase activity was higher at the last days of storage, regardless if the fruits were immersed or not in hot water. However, the catalase activity was higher in ‘Mammoth Spineless’, which presented the higher tolerance to chilling injury. The fruit skin browning was coincident with the increase on polyphenoloxidase activity and phenolic compounds content. This relationship was not observed for the peroxidase activity. The heat treatment during 15 minutes increased the length of storage of cultivar Amarelinho, and for 30 minutes of the cultivar Mammoth Spineless.