cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/hfnsh-mp818
title: COS7 tfx CHMP4A and VPS4B(E235Q)

description: Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)
description: <strong>Tilt Series Date:</strong> 2012-03-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> lipofectamine transfection</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-9/rawdata/</p>12mar14_c121_1_Cos74ADN_AT1bt10019.mrc,  Tilt Series (Pixel Size 0.9 nm), 561.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-9/rawdata/12mar14_c121_1_Cos74ADN_AT1bt10019.mrc"
        > <i class="download icon"></i> Download </a></p>

        12mar14_c121_1_Cos74ADN_AT1bt10019_full.rec,  Reconstruction (Pixel Size 1.8 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-14-9/3dimage_17749/12mar14_c121_1_Cos74ADN_AT1bt10019_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.34740/kaggle/dsv/12872977
title: Newa News Dataset in Pracalit/Prachalit Script
DOI: 10.22002/a29q8-y5v22
title: Vibrio delta-CheA3

description: Vibrio cholerae strain deleted for CheA3
description: <strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O1 biovar El Tor</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Ikuro Kawagishi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-05-8/rawdata/</p>deltaA3VibrioC0004.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-05-8/rawdata/deltaA3VibrioC0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        deltaA3VibrioC0004_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-03-05-8/3dimage_39729/deltaA3VibrioC0004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pc1v3-n2g41
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C123
description: <strong>Tilt Series Date:</strong> 2012-03-28</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-35/3dimage_69162/</p>12mar28_pnlDN_C123_1_AT7BT1_0004_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-35/3dimage_69162/12mar28_pnlDN_C123_1_AT7BT1_0004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12mar28_pnlDN_C123_1_AT7BT1_0004.mrc,  Tilt Series (Pixel Size 0.5 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-03-28-35/rawdata/12mar28_pnlDN_C123_1_AT7BT1_0004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/16bb4-z5k76
title: HUVECs 24h PI pNLBglI

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 6500
description: <strong>Tilt Series Date:</strong> 2013-04-30</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 6500x. </p>
<strong>Microscope:</strong> UCLA Titan</p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> FEIbatchtomo</p>
<strong>Collaborators and Roles:</strong> Collected at UCLA with FEI Batchtomo</p>
<strong>Sample Preparation:</strong> HUVECs imaged 24 hours after infection with pNLBglI. The goal is to image maturing HIV at the edge of infected cells.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-17/rawdata/</p>13apr26_tomo_23.mrc,  Tilt Series (Pixel Size 1.35 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-17/rawdata/13apr26_tomo_23.mrc"
        > <i class="download icon"></i> Download </a></p>

        13apr26_tomo_23_full.rec,  Reconstruction (Pixel Size 2.7 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-04-30-17/3dimage_26334/13apr26_tomo_23_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.a3bxxr
title: Occurrence Download

description: A dataset containing 17 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Hibiscus aridus"
 ]
}

 The dataset includes 17 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045838-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/mcvz8-v6379
title: HUVECs infx pNLGFP

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 19000.
description: <strong>Tilt Series Date:</strong> 2013-07-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>
<strong>Sample Preparation:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-06-20/rawdata/</p>13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0032.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-06-20/rawdata/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0032.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0032_part121_63.rec,  Reconstruction (Pixel Size 2.24 nm), 602.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-06-20/3dimage_28099/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0032_part121_63.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.tgyu8h
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Atoconeura kenya, Atoconeura, Atoconeura eudoxia, Atoconeura aethiopica, Atoconeura luxata, Atoconeura biordinata, Allocnemis cyanura, Allocnemis, Trithemis, Elattoneura villiersi, Elattoneura, Thermochoria equivocata, Thermochoria, Thermochoria jeanneli, Ischnura elegans, Ischnura, Selysiothemis nigra, Selysiothemis, Hemistigma affine, Hemistigma, Aciagrion, Sympetrum fonscolombii, Sympetrum, Micromacromia camerunica, Micromacromia, Micromacromia zygoptera, Aethiothemis erythromelas, Aethiothemis, Aciagrion gracile, Aciagrion dondoense, Trithetrum navasi, Trithetrum, Trithetrum congoense, Aciagrion africanum, Trithemis tropicana, Hadrothemis vrijdaghi, Hadrothemis, Hadrothemis scabrifrons, Hadrothemis defecta, Hadrothemis camarensis, Hadrothemis versuta, Hadrothemis infesta, Hadrothemis coacta, Hemicordulia, Diastatomma multilineatum, Diastatomma, Mastigogomphus chapini, Mastigogomphus, Crenigomphus kavangoensis, Crenigomphus, Pseudagrion merina, Pseudagrion, Allocnemis marshalli, Hemicordulia asiatica, Microgomphus, Cordulegaster boltonii algirica, Cordulegaster boltonii, Cordulegaster, Hemicordulia similis, Allocnemis elongata, Orthetrum malgassicum, Orthetrum, Lestinogomphus silkeae, Lestinogomphus, Hemicordulia virens, Microgomphus nyassicus, Paragomphus serrulatus, Paragomphus, Aeshna isoceles, Aeshna, Neurocordulia, Mortonagrion stygium, Mortonagrion, Pyrrhosoma nymphula, Pyrrhosoma, Proplatycnemis pseudalatipes, Proplatycnemis, Neophya rutherfordi, Neophya, Allocnemis superba, Elattoneura vittata, Onychogomphus nigrotibialis, Onychogomphus, Cordulia, Cordulia aenea, Stenocypha molindica, Stenocypha, Platycypha picta, Platycypha, Syncordulia venator, Syncordulia, Syncordulia gracilis, Crocothemis, Metacnemis valida, Metacnemis, Crocothemis sanguinolenta, Crocothemis erythraea, Platycypha rufitibia, Crocothemis saxicolor, Crocothemis striata)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((45.66 11.3473,45.0861 11.0599,46.1382 11.0599,46.1382 11.2722,45.66 11.3473))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045837-250811113504898/datasets/export for details.
DOI: 10.22002/c8x73-rhw31
title: Alongum

description: Tprimitia_lysozyme
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-137/rawdata/</p>trepLys2a0001.mrc,  Tilt Series (Pixel Size 0.95 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-137/rawdata/trepLys2a0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        trepLys2a0001_full.rec,  Reconstruction (Pixel Size 1.95 nm), 412.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-137/3dimage_56991/trepLys2a0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/cjame-7m770
title: PAO1 Wild Type

description: Pseudomonas aeruginosa PAO1 Wild Type
description: <strong>Tilt Series Date:</strong> 2014-10-14</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Kylie Watts</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-24/rawdata/</p>PAOIWTMCTest0014_mcorr.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-24/rawdata/PAOIWTMCTest0014_mcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        PAOIWTMCTest0014_mcorr_full.rec,  Reconstruction (Pixel Size 1.28 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-10-14-24/3dimage_47063/PAOIWTMCTest0014_mcorr_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/htth5-zk089
title: UU 2804- A413T CheA deltaP2

description: E. coli strain A413T (locked in CheA off state) with deletion of P2 in CheA
description: <strong>Tilt Series Date:</strong> 2012-08-31</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo/IMOD/tomo3D</p>
<strong>Collaborators and Roles:</strong> John S. Parkinson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB media, lysed with penicillin for one hour</p>
<strong>Sample Preparation:</strong> plunge frozen on R2/2 Quantifoil grids in liquid ethane/propane</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-7/rawdata/</p>2804atlas10007.mrc,  Tilt Series (Pixel Size 0.33 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-7/rawdata/2804atlas10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        2804atlas10007_full.rec,  Reconstruction (Pixel Size 0.66 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-08-31-7/3dimage_20584/2804atlas10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t9ryx-byn02
title: HUVECs infx pNLGFP

description: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).         dosage: 150/A2. defocus: -6um. magnification: 19000.
description: <strong>Tilt Series Date:</strong> 2013-07-03</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen UCLA)</p>
<strong>Sample Preparation:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-03-39/rawdata/</p>13jul03_HUVECspnl24hPI_montage_33x33_0042.mrc,  Tilt Series (Pixel Size 0.56 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-03-39/rawdata/13jul03_HUVECspnl24hPI_montage_33x33_0042.mrc"
        > <i class="download icon"></i> Download </a></p>

        13jul03_HUVECspnl24hPI_montage_33x33_0042_full.rec,  Reconstruction (Pixel Size 2.24 nm), 602.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-03-39/3dimage_27989/13jul03_HUVECspnl24hPI_montage_33x33_0042_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jqqyz-kae69
title: Agro_C58

description: Agro_liq_22C
description: <strong>Tilt Series Date:</strong> 2014-06-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AB media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-06-27-1/rawdata/</p>c58_22_liqPl0002.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-06-27-1/rawdata/c58_22_liqPl0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        2_cut_full.rec,  Reconstruction (Pixel Size 2.0 nm), 727.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-06-27-1/3dimage_56634/2_cut_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gyare-vgk27
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN and pNLGFP provirus construct
description: <strong>Tilt Series Date:</strong> 2012-04-25</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-5/3dimage_68540/</p>12apr25_C123_3_Cos7pNLDN_AT3BT1_0002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
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