cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.rmww83
title: Occurrence Download

description: A dataset containing 1283 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Limonium dodartii (Girard) Kuntze"
 ]
}

 The dataset includes 1283 records from 95 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000160-250117103327516/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.7483/opendata.cms.k0wm.i185
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-150_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.7483/opendata.cms.x3on.915e
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-60_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.7483/opendata.cms.dzlq.8728
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-60_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.6084/m9.figshare.28211267
title: <i>MDM2</i> polymorphisms and hematological parameters during chemotherapy, raw data and ratios

description: <i>MDM2</i> polymorphism status, raw data (hematological parameters) and ratios of hematological parameters for all patients in the DDP trial. Hematological parameters from blood samples collected before each cycle of chemotherapy (8 in total, 4 courses of epirubicin, followed by 4 courses of docetaxel, unless otherwise specified in the “notes” section), annotated as x(number of cycle)_Hb (hemoglobin)/LPK (total leukocyte count)/Neu (neutrophil count)/TPK (thrombocyte count). Calculated ratios for all hematological parameters are annotated as Cx(numerator)Cx(denominator), e.g. the ratio of neutrophil counts before the 4<sup>th</sup> course of chemotherapy divided by the pre treatment neutrophil count is annotated C4C1. R<sub>epi,</sub> R<sub>doc</sub> and R<sub>tot</sub> , the ratios used in statistical analysis, represent the ratio of cell counts pre and post treatment with epirubicin (C5C1) and docetaxel (C8C5) respectively, while R<sub>tot</sub> (C8C1) represents the ratio of cell counts before and after completing all 8 courses of chemotherapy. Patients are presented in order by SNP309 genotype, then by del1518 and SNP285 genotype.
DOI: 10.6084/m9.figshare.28211267.v1
title: <i>MDM2</i> polymorphisms and hematological parameters during chemotherapy, raw data and ratios

description: <i>MDM2</i> polymorphism status, raw data (hematological parameters) and ratios of hematological parameters for all patients in the DDP trial. Hematological parameters from blood samples collected before each cycle of chemotherapy (8 in total, 4 courses of epirubicin, followed by 4 courses of docetaxel, unless otherwise specified in the “notes” section), annotated as x(number of cycle)_Hb (hemoglobin)/LPK (total leukocyte count)/Neu (neutrophil count)/TPK (thrombocyte count). Calculated ratios for all hematological parameters are annotated as Cx(numerator)Cx(denominator), e.g. the ratio of neutrophil counts before the 4<sup>th</sup> course of chemotherapy divided by the pre treatment neutrophil count is annotated C4C1. R<sub>epi,</sub> R<sub>doc</sub> and R<sub>tot</sub> , the ratios used in statistical analysis, represent the ratio of cell counts pre and post treatment with epirubicin (C5C1) and docetaxel (C8C5) respectively, while R<sub>tot</sub> (C8C1) represents the ratio of cell counts before and after completing all 8 courses of chemotherapy. Patients are presented in order by SNP309 genotype, then by del1518 and SNP285 genotype.
DOI: 10.7483/opendata.cms.cfvc.fsj0
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-70_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.7483/opendata.cms.bnpw.fuu3
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-70_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.7483/opendata.cms.gvwv.so7x
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-80_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.7483/opendata.cms.gvsr.ttrg
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-80_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.7483/opendata.cms.ri42.bhut
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-90_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.7483/opendata.cms.4ldg.45qj
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-300_MY-90_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.7483/opendata.cms.y1ej.q2j9
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-320_MY-100_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.7483/opendata.cms.hnh7.1elz
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-320_MY-100_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.6084/m9.figshare.24315616
title: The effect of migratory beekeeping on Indian honey bee (<i>Apis cerana indica</i>) microbiota – a first report

description: Migratory beekeeping is a widely extended practice aimed at increasing the yield of honey and pollination services. Microbial symbionts that inhabit the honey bee’s gut are essential to their host’s food digestion, immunity, and gut protection. Throughout the honey bee life cycle and at several migratory sites, the taxonomic composition of the gut microbiota changes. However, it is yet unknown how significant changes in migratory locations, such as altered floral supply, parasitism and pathogen infestation, affect the dynamics of the gut microbiota. The goal of this study was to describe the Indian honey bee (<i>Apis cerana indica</i>) gut microbiome at different migratory sites of Puducherry and Tamil Nadu. Our results found that the core bacterial composition of the microbiota was consistent over different migratory sites. Despite this stability, our comparisons of the microbiota of honey bees from the same colony show that there are differences in composition at different migratory sites. Likewise, there is variation in relative abundance between microbiomes of different migratory sites. The four most dominant phyla were Proteobacteria followed by Bacteroidota, Actinobacteriota and Firmicutes. Most of the isolates belonged to families of Xanthomonadaceae, Orbaceae, Weeksellaceae, Bifidobacteriaceae, Rhizobiaceae, Neisseriaceae, Lactobacillaceae, Dysgonomonadaceae, Clostridiaceae and Enterococcaceae. However, several commensal bacteria that proliferate in the hive environment including members of Lactobacillaceae (<i>Lactobacillus kunkeei</i>), Bifidobacteriaceae, Orbaceae and Neisseriaceae were also identified and considered as beneficial gut inhabitants and involved in the maintenance of a healthy microbiota. This is the first report on bee gut microbiota from Puducherry and Tamil Nadu geographically situated at the southeast coast of India.
DOI: 10.6084/m9.figshare.24315616.v1
title: The effect of migratory beekeeping on Indian honey bee (<i>Apis cerana indica</i>) microbiota – a first report

description: Migratory beekeeping is a widely extended practice aimed at increasing the yield of honey and pollination services. Microbial symbionts that inhabit the honey bee’s gut are essential to their host’s food digestion, immunity, and gut protection. Throughout the honey bee life cycle and at several migratory sites, the taxonomic composition of the gut microbiota changes. However, it is yet unknown how significant changes in migratory locations, such as altered floral supply, parasitism and pathogen infestation, affect the dynamics of the gut microbiota. The goal of this study was to describe the Indian honey bee (<i>Apis cerana indica</i>) gut microbiome at different migratory sites of Puducherry and Tamil Nadu. Our results found that the core bacterial composition of the microbiota was consistent over different migratory sites. Despite this stability, our comparisons of the microbiota of honey bees from the same colony show that there are differences in composition at different migratory sites. Likewise, there is variation in relative abundance between microbiomes of different migratory sites. The four most dominant phyla were Proteobacteria followed by Bacteroidota, Actinobacteriota and Firmicutes. Most of the isolates belonged to families of Xanthomonadaceae, Orbaceae, Weeksellaceae, Bifidobacteriaceae, Rhizobiaceae, Neisseriaceae, Lactobacillaceae, Dysgonomonadaceae, Clostridiaceae and Enterococcaceae. However, several commensal bacteria that proliferate in the hive environment including members of Lactobacillaceae (<i>Lactobacillus kunkeei</i>), Bifidobacteriaceae, Orbaceae and Neisseriaceae were also identified and considered as beneficial gut inhabitants and involved in the maintenance of a healthy microbiota. This is the first report on bee gut microbiota from Puducherry and Tamil Nadu geographically situated at the southeast coast of India.
DOI: 10.7483/opendata.cms.u90l.f6sg
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-320_MY-125_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16MiniAODv2-106X_mcRun2_asymptotic_v17-v2/MINIAODSIM
DOI: 10.6084/m9.figshare.23926014
title: Short-term effects of <i>Brevibacillus laterosporus</i> supplemented diet on worker honey bee microbiome: a pilot study

description: In the current study, honey bees’ diet was supplemented with spores of <i>Brevibacillus laterosporus</i> BGSP11 at concentration of 10<sup>8</sup> CFU/ml in sucrose solution and its short-term effects on their micro- and mycobiota have been analyzed using Illumina MiSeq sequencing. Obtained results indicate that this treatment does not lead to potentially harmful changes in the bacterial microbiome of worker bees, slightly affecting the composition of core microbiota. Moreover, several potentially beneficial changes have been observed. The treatment has led to a significant increase in the abundance of <i>Snodgrassella alvi,</i> and species from <i>Lactobacillus</i> and <i>Bifidobacterium</i> genera which play important roles in protection against several honey bee pathogens. Simultaneously, <i>B. laterosporus</i> enriched diet have led to almost complete eradication of <i>Enterobacteriaceae</i> family, the taxon that contains several putative pathogen species. On the other hand, the treatment affected mycobiota more profoundly, which was expected considering the greater instability compared to microbiota. Although the observed changes in honey bee mycobiome cannot be considered <i>a priori</i> beneficial or harmful, since the interaction between the bee and its mycobiome has not been sufficiently studied, certain beneficial consequences of the treatment have been observed. They are primarily reflected in the reduction of phytopathogenic fungi that can affect the organoleptic and techno-functional characteristics of honey. In addition, before introducing <i>B. laterosporus</i> in beekeeping practice as a biological agent for pathogen control it is necessary to perform more thorough studies of the impact on the honey bee microbiome, immune system, physiology and economic characteristics of honey bee colonies.
DOI: 10.6084/m9.figshare.23926014.v1
title: Short-term effects of <i>Brevibacillus laterosporus</i> supplemented diet on worker honey bee microbiome: a pilot study

description: In the current study, honey bees’ diet was supplemented with spores of <i>Brevibacillus laterosporus</i> BGSP11 at concentration of 10<sup>8</sup> CFU/ml in sucrose solution and its short-term effects on their micro- and mycobiota have been analyzed using Illumina MiSeq sequencing. Obtained results indicate that this treatment does not lead to potentially harmful changes in the bacterial microbiome of worker bees, slightly affecting the composition of core microbiota. Moreover, several potentially beneficial changes have been observed. The treatment has led to a significant increase in the abundance of <i>Snodgrassella alvi,</i> and species from <i>Lactobacillus</i> and <i>Bifidobacterium</i> genera which play important roles in protection against several honey bee pathogens. Simultaneously, <i>B. laterosporus</i> enriched diet have led to almost complete eradication of <i>Enterobacteriaceae</i> family, the taxon that contains several putative pathogen species. On the other hand, the treatment affected mycobiota more profoundly, which was expected considering the greater instability compared to microbiota. Although the observed changes in honey bee mycobiome cannot be considered <i>a priori</i> beneficial or harmful, since the interaction between the bee and its mycobiome has not been sufficiently studied, certain beneficial consequences of the treatment have been observed. They are primarily reflected in the reduction of phytopathogenic fungi that can affect the organoleptic and techno-functional characteristics of honey. In addition, before introducing <i>B. laterosporus</i> in beekeeping practice as a biological agent for pathogen control it is necessary to perform more thorough studies of the impact on the honey bee microbiome, immune system, physiology and economic characteristics of honey bee colonies.
DOI: 10.6084/m9.figshare.24968057
title: High detection rate of the virulence plasmid pMP19 from <i>Melissococcus plutonius</i>-positive honey and larvae samples

description: European foulbrood caused by <i>Melissococcus plutonius</i> is a serious infectious disease of honey bee brood. pMP19 is known as an important virulence plasmid of <i>M. plutonius</i>. Nevertheless, pMP19-negative <i>M. plutonius</i> has often been isolated from EFB cases. Since pMP19 is unstable in <i>M. plutonius</i> during propagation in laboratories, such pMP19-negative isolates may have lost this plasmid during the cultivation steps for isolation. Therefore, the retention rate of pMP19 in <i>M. plutonius</i> in the field may be higher than that of isolates. To gain insight into the distribution of pMP19-positive <i>M. plutonius</i> in the field, we directly detected pMP19 by PCR from 103 Japanese honey and 41 diseased larvae samples that were known to be contaminated by <i>M. plutonius</i> and revealed that all the <i>M. plutonius</i>-positive samples were also positive for pMP19. These results suggest high pMP19 prevalence in <i>M. plutonius</i> in the field and provide deeper insight into this disease.
DOI: 10.6084/m9.figshare.24968057.v1
title: High detection rate of the virulence plasmid pMP19 from <i>Melissococcus plutonius</i>-positive honey and larvae samples

description: European foulbrood caused by <i>Melissococcus plutonius</i> is a serious infectious disease of honey bee brood. pMP19 is known as an important virulence plasmid of <i>M. plutonius</i>. Nevertheless, pMP19-negative <i>M. plutonius</i> has often been isolated from EFB cases. Since pMP19 is unstable in <i>M. plutonius</i> during propagation in laboratories, such pMP19-negative isolates may have lost this plasmid during the cultivation steps for isolation. Therefore, the retention rate of pMP19 in <i>M. plutonius</i> in the field may be higher than that of isolates. To gain insight into the distribution of pMP19-positive <i>M. plutonius</i> in the field, we directly detected pMP19 by PCR from 103 Japanese honey and 41 diseased larvae samples that were known to be contaminated by <i>M. plutonius</i> and revealed that all the <i>M. plutonius</i>-positive samples were also positive for pMP19. These results suggest high pMP19 prevalence in <i>M. plutonius</i> in the field and provide deeper insight into this disease.
DOI: 10.7483/opendata.cms.hwmy.7jml
title: /NMSSM_XToYHTo2G2WTo2G4Q_MX-320_MY-125_TuneCP5_13TeV-madgraph-pythia8/RunIISummer20UL16NanoAODv9-106X_mcRun2_asymptotic_v17-v1/NANOAODSIM
DOI: 10.6084/m9.figshare.23925389
title: Old strategies as modern solutions, shook swarming reduced <i>Varroa destructor</i> infestation rates of managed honey bee, <i>Apis mellifera</i>, colonies

description: Transferring adult honey bees from the combs of their hive to a new empty hive is a management strategy known as “shook swarming” that has been used by beekeepers in a variety of contexts since as early as 1796. <i>Varroa destructor</i> are dependent on mature brood to reproduce and shook swarming breaks the colony’s brood cycle by separating the adult bees from the brood developing in the old combs. We investigated the efficacy and practicality of shook swarming to mitigate negative health effects of Varroa in honey bee colonies by reducing Varroa infestation rates. New colonies were founded <i>via</i> traditional splitting or the shook swarm technique either with or without comb in the new hive. All colonies were given ∼1 month to establish and then assessed for foraging activity, colony strength, and Varroa infestation rate. We observed a significant decrease in the Varroa infestation rates of colonies that experienced shook swarming compared with those that had not. Colonies established <i>via</i> shook swarming had lower colony strength scores but maintained similar relative foraging activity as traditional splits. Our findings indicate that shook swarming is a viable, non-chemical control strategy for managing Varroa infestations and should be considered in long-term management plans where colonies undergo shook swarming aligned with honey production and/or pollination service delivery.
DOI: 10.6084/m9.figshare.23925389.v1
title: Old strategies as modern solutions, shook swarming reduced <i>Varroa destructor</i> infestation rates of managed honey bee, <i>Apis mellifera</i>, colonies

description: Transferring adult honey bees from the combs of their hive to a new empty hive is a management strategy known as “shook swarming” that has been used by beekeepers in a variety of contexts since as early as 1796. <i>Varroa destructor</i> are dependent on mature brood to reproduce and shook swarming breaks the colony’s brood cycle by separating the adult bees from the brood developing in the old combs. We investigated the efficacy and practicality of shook swarming to mitigate negative health effects of Varroa in honey bee colonies by reducing Varroa infestation rates. New colonies were founded <i>via</i> traditional splitting or the shook swarm technique either with or without comb in the new hive. All colonies were given ∼1 month to establish and then assessed for foraging activity, colony strength, and Varroa infestation rate. We observed a significant decrease in the Varroa infestation rates of colonies that experienced shook swarming compared with those that had not. Colonies established <i>via</i> shook swarming had lower colony strength scores but maintained similar relative foraging activity as traditional splits. Our findings indicate that shook swarming is a viable, non-chemical control strategy for managing Varroa infestations and should be considered in long-term management plans where colonies undergo shook swarming aligned with honey production and/or pollination service delivery.
DOI: 10.6084/m9.figshare.24788001
title: Moderate use of pesticides in allotment gardens as indicated by their residues in larval food provisions of <i>Osmia</i> solitary bees

description: Exposure to pesticides may jeopardize pollinators, but it has rarely been examined in urban landscapes. We used larval food provisions (mix of pollen and nectar) of mason bees (<i>Osmia</i> spp.) nested in artificial shelters as matrices to investigate the rate of pesticide pollution in twelve allotment sites in Prague and Brno cities. Seven commercial croplands (fruit orchards, oilseed rape fields) were selected for comparison. In total, 79 samples of larval provisions were analysed using a validated UPLC–MS/MS method for the presence of 262 pesticides and/or important metabolites. Fifty-six pesticide residues were detected in the allotments and 74 in cropland sites. Fifty pesticide compounds were common to both landscapes. Fewer residues were found in the allotments (14.3 ± 0.9, mean ± SE) than in croplands (21.5 ± 1.1, mean ± SE; df = 17.4; t = −2.9; <i>p</i> &lt; 0.01), and they were present at low levels up to 16 ppb. In cropland sites, ten residues occurred at high individual concentrations (mean per site &gt; 10 ppb). The highest levels were observed for the fungicide pyrimethanil (mean = 1,989 ppb, maximum = 3,983 ppb) and among insecticides for thiacloprid (mean = 136 ppb, maximum = 170 ppb). The results supported the presumption of a lower risk of pesticide exposure in urban gardens than in croplands. Given the limited knowledge on the sublethal effects of pesticides and especially pesticide mixtures on solitary bee development, the regulation or control of pesticide use in allotment gardens is recommended.
DOI: 10.6084/m9.figshare.24788001.v1
title: Moderate use of pesticides in allotment gardens as indicated by their residues in larval food provisions of <i>Osmia</i> solitary bees

description: Exposure to pesticides may jeopardize pollinators, but it has rarely been examined in urban landscapes. We used larval food provisions (mix of pollen and nectar) of mason bees (<i>Osmia</i> spp.) nested in artificial shelters as matrices to investigate the rate of pesticide pollution in twelve allotment sites in Prague and Brno cities. Seven commercial croplands (fruit orchards, oilseed rape fields) were selected for comparison. In total, 79 samples of larval provisions were analysed using a validated UPLC–MS/MS method for the presence of 262 pesticides and/or important metabolites. Fifty-six pesticide residues were detected in the allotments and 74 in cropland sites. Fifty pesticide compounds were common to both landscapes. Fewer residues were found in the allotments (14.3 ± 0.9, mean ± SE) than in croplands (21.5 ± 1.1, mean ± SE; df = 17.4; t = −2.9; <i>p</i> &lt; 0.01), and they were present at low levels up to 16 ppb. In cropland sites, ten residues occurred at high individual concentrations (mean per site &gt; 10 ppb). The highest levels were observed for the fungicide pyrimethanil (mean = 1,989 ppb, maximum = 3,983 ppb) and among insecticides for thiacloprid (mean = 136 ppb, maximum = 170 ppb). The results supported the presumption of a lower risk of pesticide exposure in urban gardens than in croplands. Given the limited knowledge on the sublethal effects of pesticides and especially pesticide mixtures on solitary bee development, the regulation or control of pesticide use in allotment gardens is recommended.
DOI: 10.6084/m9.figshare.27178703
title: Introgressive hybridisation puts the distinctive population of <i>Apis mellifera mellifera</i> in Ireland at risk: evidence from a multidisciplinary approach

description: <i>Apis mellifera mellifera</i> a honey bee subspecies at risk of extinction across much of its range has a stronghold in Ireland where it is the native honey bee subspecies and is referred to as the black bee. However, an increase in imports of other subspecies and commercial strains is now a major threat to its genetic integrity. Furthermore, despite assertions by Irelands’ beekeepers that the black bee is a distinct ecotype, little taxonomic work has focused on this population. Here we liaise with beekeepers and assess the managed honey bee cohort for signs of increased introgression. We also investigate morphological variation in <i>A. m. mellifera</i> colonies to establish for the first time a description of this subspecies in Ireland. SNPs and mitochondrial data indicate a further increase in introgression in the population even though much of the population remains <i>A. m. mellifera</i>. The morphology of Irish <i>A. m. mellifera</i> has deviated significantly from European populations and can be characterised as being smaller and hairier, with a broad abdomen, shorter legs and a shorter proboscis. Their fore wings are longer but with a smaller cubital index. These attributes may have evolved as adaptations to the unique environmental and climatic conditions found in Ireland but may also have been influenced by artificial selection and/or genetic drift.