cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5634655.v1
title: MICROEMULSION OF BRAZIL NUT OIL AS A NATURAL PRODUCT TO IMPROVE SUPEROXIDE RELEASE IN HUMAN PHAGOCYTES

description: The aim of this study was to develop and characterize a Brazil nut oil microemulsion system and determine the effect of this microemulsion on the superoxide release in human phagocytes. The microemulsion was formulated using distilled water, Brazil nut as the oily phase, Sorbitan monooleate, Polysorbate 80, and butan-1-ol. Hydrophile-lipophile balance of Brazil nut oil was determined, pseudoternary diagrams were prepared, and microemulsion diagram regions were preselected. Preliminary and accelerated stability tests, rheological characterization and dynamic light scattering were performed. The immunomodulatory effect of the microemulsion on the interactions with leukocytes was determined by superoxide release. The developed formulations were classified as oil-in-water, showed a Newtonian profile, with linear viscosity and droplet sizes of 46.9; 66.5 and 34.3 nm. When we assessed the interaction of the microemulsion of Brazil nut oil with phagocytes, we observed an increase in superoxide release. Our findings indicate that the developed formulation improved the immunomodulatory activity of the Brazil nut oil, proving to be a natural product option for future applications, in particular for infectious diseases.
DOI: 10.6084/m9.figshare.5634652.v1
title: BIOSYNTHESIS OF INDUSTRIAL ENZYMES BY FREE AND IMMOBILIZED Alicyclobacillus spp IN DIFFERENT MATRICES AND THE USE OF ULTRAFILTRATION IN THE ENZYMES CONCENTRATION

description: The biosynthesis of amylase and collagenase, produced by A. acidocaldarius and A. sendaiensis respectively, were studied, and different matrices evaluated for the microorganisms immobilization and enzymes production optimization, such as loofa sponge, alginate-sponge and alginate, followed by concentration through ultrafiltration. Using a wheat bran substrate, the amylase enzyme displayed enzymatic activity of 0.45 U mL-1 and optimum temperature and pH conditions of 75 °C and pH 3.0, respectively. Thermal stability was in the range of 55 to 60 °C. The apparent Km and Vmax were 3.2 mg mL-1 and 0.5 U mL-1, respectively. The production of collagenase by A. sendaiensis was carried out with potato dextrose broth substrate and the activity obtained was 7.2 U mL-1. For amylase, the best results were obtained from immobilization in loofa sponge and the use of ultrafiltration (0.67 U mL-1) and for the collagenase extract, from the free biomass and ultrafiltration (13.6 U mL-1). The use of an ultrafiltration system enabled an average increase of 54% in the activity of both enzymes. Therefore, Alicyclobacillus are capable of producing enzymes of industrial interest, with the possibility of economically viable application of the substrate, and the use of immobilization and ultrafiltration produced positive results.
DOI: 10.6084/m9.figshare.5634649
title: Decellularization of placentas: establishing a protocol

description: Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for further analyses using histology, scanning electron microscopy, immunofluorescence and DNA quantification. Sodium dodecyl sulfate (SDS) was the most effective detergent for cell removal. Freezing placentas before decellularization required longer periods of incubation in different detergents. Both perfusion and immersion methods were capable of removing cells. Placentas decellularized using Protocol I (1% SDS, 5 mM EDTA, 50 mM TRIS, and 0.5% antibiotic) preserved the ECM structure better, but Protocol I was less efficient to remove cells and DNA content from the ECM than Protocol II (1% SDS, 5 mM EDTA, 0.05% trypsin, and 0.5% antibiotic).
DOI: 10.6084/m9.figshare.5634649.v1
title: Decellularization of placentas: establishing a protocol

description: Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for further analyses using histology, scanning electron microscopy, immunofluorescence and DNA quantification. Sodium dodecyl sulfate (SDS) was the most effective detergent for cell removal. Freezing placentas before decellularization required longer periods of incubation in different detergents. Both perfusion and immersion methods were capable of removing cells. Placentas decellularized using Protocol I (1% SDS, 5 mM EDTA, 50 mM TRIS, and 0.5% antibiotic) preserved the ECM structure better, but Protocol I was less efficient to remove cells and DNA content from the ECM than Protocol II (1% SDS, 5 mM EDTA, 0.05% trypsin, and 0.5% antibiotic).
DOI: 10.6084/m9.figshare.5634646
title: GC-FID METHODOLOGY VALIDATION FOR THE FATTY ESTERS CONTENT DETERMINATION IN BIODIESEL WITH HEXADECYL ACETATE AS THE INTERNAL STANDARD

description: Biodiesel purity expressed as fatty esters content is one of its most important quality parameters. Although several instrumental methods have been employed, gas chromatography with internal standard calibration has been the most used. Biodiesel is a very complex matrix, therefore finding a suitable internal standard (IS) is not straightforward. EN14103:2003 standard established methyl heptadecanoate (C17:0) as IS. Since this fatty ester is naturally present in some biodiesel feedstock as tallow, that standard was reviewed in 2011, and methyl nonadecanoate (C19:0) became the new IS. In turn, C19:0 produces a peak on the chromatogram that is difficult to resolve from methyl linoeate (C18:2) and metyl linolenate (C18:3) ones. In this paper, a GC-FID method for the determination of the esters content in methyl and ethyl biodiesel from canola and soy oils, using hexadecyl acetate as a new internal standard was validated. EN14103:2003 was used as reference method. Method selectivity, accuracy (t student parameter &lt; 2.18 from both methods), repeatability (0.1 - 0.4%), intermediate precision (0.2 - 1.8%), and robustness (0.1 - 1.6%) were investigated and considered appropriate for the scope.
DOI: 10.6084/m9.figshare.5634646.v1
title: GC-FID METHODOLOGY VALIDATION FOR THE FATTY ESTERS CONTENT DETERMINATION IN BIODIESEL WITH HEXADECYL ACETATE AS THE INTERNAL STANDARD

description: Biodiesel purity expressed as fatty esters content is one of its most important quality parameters. Although several instrumental methods have been employed, gas chromatography with internal standard calibration has been the most used. Biodiesel is a very complex matrix, therefore finding a suitable internal standard (IS) is not straightforward. EN14103:2003 standard established methyl heptadecanoate (C17:0) as IS. Since this fatty ester is naturally present in some biodiesel feedstock as tallow, that standard was reviewed in 2011, and methyl nonadecanoate (C19:0) became the new IS. In turn, C19:0 produces a peak on the chromatogram that is difficult to resolve from methyl linoeate (C18:2) and metyl linolenate (C18:3) ones. In this paper, a GC-FID method for the determination of the esters content in methyl and ethyl biodiesel from canola and soy oils, using hexadecyl acetate as a new internal standard was validated. EN14103:2003 was used as reference method. Method selectivity, accuracy (t student parameter &lt; 2.18 from both methods), repeatability (0.1 - 0.4%), intermediate precision (0.2 - 1.8%), and robustness (0.1 - 1.6%) were investigated and considered appropriate for the scope.
DOI: 10.6084/m9.figshare.5634643
title: PHOSPHOR SCREENS FOR IMAGE PROJECTION

description: In this work we describe a methodology to obtain phosphor screens used in various applications to produce images or patterns such as detectors and microscopes. The main advantages of these screens are the particle free and self-heating properties that allows it to be used in controlled environments minimizing the adsorbed gases in the surface of the screen. A polyvinyl alcohol deposited on top of the phosphor layer acts as an adhesive layer preventing the removal of phosphor particles from the screen surface that could result in measurement environment contamination. The tin oxide layer has a high melting point enabling the self-heating. These characteristics on screens are important for ultra-high vacuum environment and to study carbon nanotube emission patterns through field emission microscopy (FEM).
DOI: 10.6084/m9.figshare.5634643.v1
title: PHOSPHOR SCREENS FOR IMAGE PROJECTION

description: In this work we describe a methodology to obtain phosphor screens used in various applications to produce images or patterns such as detectors and microscopes. The main advantages of these screens are the particle free and self-heating properties that allows it to be used in controlled environments minimizing the adsorbed gases in the surface of the screen. A polyvinyl alcohol deposited on top of the phosphor layer acts as an adhesive layer preventing the removal of phosphor particles from the screen surface that could result in measurement environment contamination. The tin oxide layer has a high melting point enabling the self-heating. These characteristics on screens are important for ultra-high vacuum environment and to study carbon nanotube emission patterns through field emission microscopy (FEM).
DOI: 10.6084/m9.figshare.5634640
title: Assessment of potential and use of the IPTU tax, based on the fuzzy set theory

description: Abstract This article proposes a comparative analysis among Brazilian municipalities in order to identify the determinants of the potential and of the ability to collect urban property tax (IPTU) in Brazil. The analysis applied the fuzzy set theory, a pioneer methodological choice in the field of public finances in Brazil, which has provided unprecedented results in terms of property taxation. The results confirmed what analysts and municipal leaders have pointed out for a long time: the majority of Brazilian municipalities do not use their full potential to collect the IPTU — a fact that tends to be more critical in smaller cities, which depend on funding from other spheres of Government. The article breaks new ground in assessing this potential based on a comparative analysis of cities with similar characteristics.
DOI: 10.6084/m9.figshare.5634640.v1
title: Assessment of potential and use of the IPTU tax, based on the fuzzy set theory

description: Abstract This article proposes a comparative analysis among Brazilian municipalities in order to identify the determinants of the potential and of the ability to collect urban property tax (IPTU) in Brazil. The analysis applied the fuzzy set theory, a pioneer methodological choice in the field of public finances in Brazil, which has provided unprecedented results in terms of property taxation. The results confirmed what analysts and municipal leaders have pointed out for a long time: the majority of Brazilian municipalities do not use their full potential to collect the IPTU — a fact that tends to be more critical in smaller cities, which depend on funding from other spheres of Government. The article breaks new ground in assessing this potential based on a comparative analysis of cities with similar characteristics.
DOI: 10.6084/m9.figshare.5634637
title: Soil management and application of agricultural gypsum in a Planosol for soybean cultivation

description: ABSTRACT: This study investigated the effects of soil management systems, tillage, and application of gypsum agricultural to soil, on soybean development in lowland areas. The experiment was carried out on an Alfisol in a randomized complete block design in a factorial arrangement. The two soil tillage practices were without deep tillage and with deep tillage. Gypsum treatments were no gypsum application, 500kg of gypsum ha-1, 1000kg of gypsum ha-1, and 1500kg of gypsum ha-1. Deep tillage resulted in less soil resistance to root penetration during ryegrass cultivation during the soybean offseason, 11 months after applying the management treatments, resulting in higher dry mass of ryegrass in the offseason and higher soybean yield in the following year.
DOI: 10.6084/m9.figshare.5634637.v1
title: Soil management and application of agricultural gypsum in a Planosol for soybean cultivation

description: ABSTRACT: This study investigated the effects of soil management systems, tillage, and application of gypsum agricultural to soil, on soybean development in lowland areas. The experiment was carried out on an Alfisol in a randomized complete block design in a factorial arrangement. The two soil tillage practices were without deep tillage and with deep tillage. Gypsum treatments were no gypsum application, 500kg of gypsum ha-1, 1000kg of gypsum ha-1, and 1500kg of gypsum ha-1. Deep tillage resulted in less soil resistance to root penetration during ryegrass cultivation during the soybean offseason, 11 months after applying the management treatments, resulting in higher dry mass of ryegrass in the offseason and higher soybean yield in the following year.
DOI: 10.6084/m9.figshare.5634634
title: Effect of Spirulina addition on the physicochemical and structural properties of extruded snacks

description: Abstract Nowadays the demand for practical food like snacks increases worldwide, however the nutritional value in most these formulations is reduced. Due to its chemical composition with high protein concentration, the microalga Spirulina has been used on the production of enriched foods. The present study aimed to evaluate the effects of Spirulina sp. LEB 18 addition on snacks formulations and extrusion conditions on the physicochemical and structural properties of snacks. Protein concentration and physical properties such as expansion index, bulk density, hardness, water absorption index, water solubility index and color were determined. The results showed that the addition of Spirulina sp. LEB 18, temperature in the last zone of the extruder and feed moisture influenced the product responses. The increase in feed moisture increased the hardness, bulk density and water absorption index of the snacks. Higher concentrations of microalga produced snacks with higher protein content, total color difference (ΔE) and compact structure. The addition of 2.6% Spirulina produced snacks with up to 11.3% protein and with adequate physical and structural properties for consumption. Thus, snacks containing Spirulina are an alternative to the demand for healthy food of practical consumption.
DOI: 10.6084/m9.figshare.5634634.v1
title: Effect of Spirulina addition on the physicochemical and structural properties of extruded snacks

description: Abstract Nowadays the demand for practical food like snacks increases worldwide, however the nutritional value in most these formulations is reduced. Due to its chemical composition with high protein concentration, the microalga Spirulina has been used on the production of enriched foods. The present study aimed to evaluate the effects of Spirulina sp. LEB 18 addition on snacks formulations and extrusion conditions on the physicochemical and structural properties of snacks. Protein concentration and physical properties such as expansion index, bulk density, hardness, water absorption index, water solubility index and color were determined. The results showed that the addition of Spirulina sp. LEB 18, temperature in the last zone of the extruder and feed moisture influenced the product responses. The increase in feed moisture increased the hardness, bulk density and water absorption index of the snacks. Higher concentrations of microalga produced snacks with higher protein content, total color difference (ΔE) and compact structure. The addition of 2.6% Spirulina produced snacks with up to 11.3% protein and with adequate physical and structural properties for consumption. Thus, snacks containing Spirulina are an alternative to the demand for healthy food of practical consumption.
DOI: 10.6084/m9.figshare.5634631
title: Short-term effects of stored homologous red blood cell transfusion on cardiorespiratory function and inflammation: an experimental study in a hypovolemia model

description: The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each animal were stored under blood bank conditions for 14 days. After 30 min of hypovolemia, the control group (n=8) received an infusion of lactated Ringer's solution (three times the removed volume). The transfusion group (n=8) received two units of homologous 14-days stored RBC and lactated Ringer's solution in a volume that was three times the difference between blood removed and blood transfusion infused. Both groups were followed up for 6 h after resuscitation with collection of hemodynamic and respiratory data. Cytokines and RNA expression were measured in plasma and lung tissue. Stored RBC transfusion significantly increased mixed oxygen venous saturation and arterial oxygen content. Transfusion was not associated with alterations on pulmonary function. Pulmonary concentrations of cytokines were not different between groups. Gene expression for lung cytokines demonstrated a 2-fold increase in mRNA level for inducible nitric oxide synthase and a 0.5-fold decrease in mRNA content for IL-21 in the transfused group. Thus, stored homologous RBC transfusion in a hypovolemia model improved cardiovascular parameters but did not induce significant effects on microcirculation, pulmonary inflammation and respiratory function up to 6 h after transfusion.
DOI: 10.6084/m9.figshare.5634631.v1
title: Short-term effects of stored homologous red blood cell transfusion on cardiorespiratory function and inflammation: an experimental study in a hypovolemia model

description: The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each animal were stored under blood bank conditions for 14 days. After 30 min of hypovolemia, the control group (n=8) received an infusion of lactated Ringer's solution (three times the removed volume). The transfusion group (n=8) received two units of homologous 14-days stored RBC and lactated Ringer's solution in a volume that was three times the difference between blood removed and blood transfusion infused. Both groups were followed up for 6 h after resuscitation with collection of hemodynamic and respiratory data. Cytokines and RNA expression were measured in plasma and lung tissue. Stored RBC transfusion significantly increased mixed oxygen venous saturation and arterial oxygen content. Transfusion was not associated with alterations on pulmonary function. Pulmonary concentrations of cytokines were not different between groups. Gene expression for lung cytokines demonstrated a 2-fold increase in mRNA level for inducible nitric oxide synthase and a 0.5-fold decrease in mRNA content for IL-21 in the transfused group. Thus, stored homologous RBC transfusion in a hypovolemia model improved cardiovascular parameters but did not induce significant effects on microcirculation, pulmonary inflammation and respiratory function up to 6 h after transfusion.
DOI: 10.6084/m9.figshare.5634628
title: Fasting: a major limitation for resistance exercise training effects in rodents

description: Protocols that mimic resistance exercise training (RET) in rodents present several limitations, one of them being the electrical stimulus, which is beyond the physiological context observed in humans. Recently, our group developed a conditioning system device that does not use electric shock to stimulate rats, but includes fasting periods before each RET session. The current study was designed to test whether cumulative fasting periods have some influence on skeletal muscle mass and function. Three sets of male Wistar rats were used in the current study. The first set of rats was submitted to a RET protocol without food restriction. However, rats were not able to perform exercise properly. The second and third sets were then randomly assigned into three experimental groups: 1) untrained control rats, 2) untrained rats submitted to fasting periods, and 3) rats submitted to RET including fasting periods before each RET session. While the second set of rats performed a short RET protocol (i.e., an adaptation protocol for 3 weeks), the third set of rats performed a longer RET protocol including overload (i.e., 8 weeks). After the short-term protocol, cumulative fasting periods promoted loss of weight (P&lt;0.001). After the longer RET protocol, no difference was observed for body mass, extensor digitorum longus (EDL) morphology or skeletal muscle function (P&gt;0.05 for all). Despite no effects on EDL mass, soleus muscle displayed significant atrophy in the fasting experimental groups (P&lt;0.01). Altogether, these data indicate that fasting is a major limitation for RET in rats.