DOI: 10.5061/dryad.sk6d5
title: Data from: Beauty in the eyes of the beholders: color vision is tuned to mate preference in the Trinidadian guppy (Poecilia reticulata)

description: A broad range of animals use visual signals to assess potential mates, and
 the theory of sensory exploitation suggests variation in visual systems
 drives mate preference variation due to sensory bias. Trinidadian guppies
 (Poecilia reticulata), a classic system for studies of the evolution of
 female mate choice, provide a unique opportunity to test this theory by
 looking for co-variation in visual tuning, light environment, and mate
 preferences. Female preference co-evolves with male coloration, such that
 guppy females from ‘low predation’ environments have stronger preferences
 for males with more orange/red coloration than do females from ‘high
 predation’ environments. Here we show that color vision also varies across
 populations, with ‘low’ predation guppies investing more of their color
 vision to detect red/orange coloration. In independently colonized
 watersheds, guppies expressed higher levels of both LWS-1 and LWS-3 (the
 most abundant LWS opsins) in ‘low predation’ populations than ‘high
 predation’ populations at a time that corresponds to differences in cone
 cell abundance. We also observed that the frequency of a coding
 polymorphism differed between high and low predation populations. Together
 this shows that the variation underlying preference could be explained by
 simple changes in expression and coding of opsins, providing important
 candidate genes to investigate the genetic basis of variation in this
 model system.
description: Gene Expression Relative
 to Average HousekeepingThis file is a cvs that
 has the level of expression of each of the 9 opsins, 1 rhodopsin and 3
 housekeeping genes measured relative to the average of the 3 housekeeping
 genes (Bactin, COI, and MyosinHC). These numbers are for each individual
 along with information on the sex, standard length (SL), population (AHP-
 Aripo High Predation, ALP- Aripo Low Predation, MHP- Marianne High
 Predation, MLP- Marianne Low Predation), Watershed, Predation regime, Day
 of collection and Time of day collected (1- 07:30, 2- 10:30, 3- 13:30, 4-
 16:30).RelHouseFinal.txtOpsin Gene Expression As Proportion of Total Opsin ExpressionThis file is a csv that has the level of expression of each of the 9 opsins measured as a proportion of total opsin expression. These numbers are for each individual along with information on the sex, standard length (SL), population (AHP- Aripo High Predation, ALP- Aripo Low Predation, MHP- Marianne High Predation, MLP- Marianne Low Predation), Watershed, Predation regime, Day of collection and Time of day collected (1- 07:30, 2- 10:30, 3- 13:30, 4- 16:30).RelOpsinFinal.txtStats in RThis file has all stats run on this project and utilizes the data sets also available in Dryad.Sandkam et al stats.RTrinidad Environmental ParametersThis is a csv file that has the raw environmental parameters measured for each of the populations on each of the 3 days. These include: Temperature (C), Conductivity, Dissolved Oxygen (DO), pH, Total Dissolved Oxygen (TDS), Salinity, and ResistivityTrinidad Environment.csvLight MeasuresThis is a csv file of the measures of lambda p50 for each population measured on each of the 3 days.lambdaP50.csvAla Ser 180 Frequency of LWS1This file has the genotype data for LWS-1 for individuals sequenced as well as the population information.Ala:Ser 180 Frequency.xlsxLWS1 AlignmentAlignment of LWS-1 sequences in FASTA format. Individuals correspond with other data sets in this Dryad file and can be correlated with environmental data presented there.LWS seq2.txt