cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6044957.v1
title: Molecular phylogeny and biogeographic history of the Neotropical tribe Glandulocaudini (Characiformes: Characidae: Stevardiinae)

description: ABSTRACT Although former studies on systematics and biogeography represent a progress on the knowledge of the tribe Glandulocaudini, none was grounded on molecular evidence. Thus, the first hypothesis of relationships for the tribe based on a multilocus analysis is presented, including all genera and most of the valid species. DNA sequences of Glandulocauda caerulea and Mimagoniates sylvicola were analyzed for the first time. A molecular clock analysis was used to estimate the origin of the Glandulocaudini and the approximate timing of cladogenetic events within the group. Glandulocaudini was recovered as monophyletic. No hypothesis recovered Glandulocauda as monophyletic, since G. melanopleura is sister to Lophiobrycon weitzmani while G. caerulea is closely related to Mimagoniates. The relationships within the latter genus were resolved. The molecular clock results indicate the origin of the Glandulocaudini during the Miocene with diversification in the group occurring from Neogene to Pleistocene. These results corroborated the hypothesis that its origin took place on the Brazilian crystalline shield with the subsequent occupation of the Atlantic Coastal drainages. Apparently, Pleistocene sea-level fluctuations might have shaped the distribution pattern of some species in Glandulocaudini.
DOI: 10.6084/m9.figshare.6044948
title: Oxytocin and arginine vasopressin systems in the domestication process

description: Abstract Domestication is of unquestionable importance to the technological revolution that has given rise to modern human societies. In this study, we analyzed the DNA and protein sequences of six genes of the oxytocin and arginine vasopressin systems (OXT-OXTR; AVP-AVPR1a, AVPR1b and AVPR2) in 40 placental mammals. These systems play an important role in the control of physiology and behavior. According to our analyses, neutrality does not explain the pattern of molecular evolution found in some of these genes. We observed specific sites under positive selection in AVPR1b (ω = 1.429, p = 0.001) and AVPR2 (ω= 1.49, p = 0.001), suggesting that they could be involved in behavior and physiological changes, including those related to the domestication process. Furthermore, AVPR1a, which plays a role in social behavior, is under relaxed selective constraint in domesticated species. These results provide new insights into the nature of the domestication process and its impact on the OXT-AVP system.
DOI: 10.6084/m9.figshare.6044948.v1
title: Oxytocin and arginine vasopressin systems in the domestication process

description: Abstract Domestication is of unquestionable importance to the technological revolution that has given rise to modern human societies. In this study, we analyzed the DNA and protein sequences of six genes of the oxytocin and arginine vasopressin systems (OXT-OXTR; AVP-AVPR1a, AVPR1b and AVPR2) in 40 placental mammals. These systems play an important role in the control of physiology and behavior. According to our analyses, neutrality does not explain the pattern of molecular evolution found in some of these genes. We observed specific sites under positive selection in AVPR1b (ω = 1.429, p = 0.001) and AVPR2 (ω= 1.49, p = 0.001), suggesting that they could be involved in behavior and physiological changes, including those related to the domestication process. Furthermore, AVPR1a, which plays a role in social behavior, is under relaxed selective constraint in domesticated species. These results provide new insights into the nature of the domestication process and its impact on the OXT-AVP system.
DOI: 10.6084/m9.figshare.6044945
title: Identification of microRNA signature in different pediatric brain tumors

description: Abstract Understanding pediatric brain tumor biology is essential to help on disease stratification, and to find novel markers for early diagnosis. MicroRNA (miRNA) expression has been linked to clinical outcomes and tumor biology. Here, we aimed to detect the expression of different miRNAs in different pediatric brain tumor subtypes to discover biomarkers for early detection and develop novel therapies. Expression of 82 miRNAs was detected in 120 pediatric brain tumors from fixed-formalin paraffin-embedded tissues, low-grade glioma, high-grade glioma, ependymoma, and medulloblastoma, using quantitative real-time PCR. Low-expression of miR-221, miR-9, and miR-181c/d and over-expression of miR-101, miR-222, miR-139, miR-1827, and miR-34c was found in medulloblastoma; low expression of miR-10a and over-expression of miR-10b and miR-29a in ependymoma; low expression of miR-26a and overexpression of miR-19a/b, miR-24, miR-27a, miR- 584, and miR-527 in low-grade glioma. Cox regression showed differential miRNA expression between responders and non-responders. The most specific were miR-10a and miR-29a low expression in LGG non-responders, miR-135a and miR-146b over-expression in ependymoma non-responders, and miR-135b overexpression in medulloblastoma non-responders. MicroRNAs are differentially expressed in subtypes of brain tumors suggesting that they may help diagnosis. A greater understanding of aberrant miRNA in pediatric brain tumors may support development of novel therapies.
DOI: 10.6084/m9.figshare.6044945.v1
title: Identification of microRNA signature in different pediatric brain tumors

description: Abstract Understanding pediatric brain tumor biology is essential to help on disease stratification, and to find novel markers for early diagnosis. MicroRNA (miRNA) expression has been linked to clinical outcomes and tumor biology. Here, we aimed to detect the expression of different miRNAs in different pediatric brain tumor subtypes to discover biomarkers for early detection and develop novel therapies. Expression of 82 miRNAs was detected in 120 pediatric brain tumors from fixed-formalin paraffin-embedded tissues, low-grade glioma, high-grade glioma, ependymoma, and medulloblastoma, using quantitative real-time PCR. Low-expression of miR-221, miR-9, and miR-181c/d and over-expression of miR-101, miR-222, miR-139, miR-1827, and miR-34c was found in medulloblastoma; low expression of miR-10a and over-expression of miR-10b and miR-29a in ependymoma; low expression of miR-26a and overexpression of miR-19a/b, miR-24, miR-27a, miR- 584, and miR-527 in low-grade glioma. Cox regression showed differential miRNA expression between responders and non-responders. The most specific were miR-10a and miR-29a low expression in LGG non-responders, miR-135a and miR-146b over-expression in ependymoma non-responders, and miR-135b overexpression in medulloblastoma non-responders. MicroRNAs are differentially expressed in subtypes of brain tumors suggesting that they may help diagnosis. A greater understanding of aberrant miRNA in pediatric brain tumors may support development of novel therapies.
DOI: 10.6084/m9.figshare.6044939
title: Morphophysiological characteristics of guanandi fruit and seeds during ripening and harvesting time

description: Abstract: The objective of this work was to determine the relationship of the morphological and physiological characteristics of guanandi (Calophyllum brasiliense) fruit and seeds with ripening and harvesting time. The reproductive cycle of a native plant population, located in the coast of the state of Paraná, Brazil, was monitored from anthesis until fruit dispersion, during three years. Monthly harvests were performed, and the morphological and physical aspects (color, size, dry mass, and moisture content) of fruit and embryos were evaluated, as well as the physiological ones (germination and vigor) of seeds. The ripening process of guanandi seeds occurred between 200 and 240 days after anthesis (DAA), with anthesis peak in February and fruit dispersion in October. The maximum dry matter mass accumulation was not synchronized with the maximum physiological potential of the seeds. Germination potential was reached close to the middle of the maturation process, and maximum vigor at the end of the cycle. Fruit external color did not change from 110 DAA onwards. The morphological characteristics related to harvesting time are fruit pulp and embryo weakly adherent to the endocarp and to the tegument, respectively, as well as the dark-brown color of these tissues.
DOI: 10.6084/m9.figshare.6044939.v1
title: Morphophysiological characteristics of guanandi fruit and seeds during ripening and harvesting time

description: Abstract: The objective of this work was to determine the relationship of the morphological and physiological characteristics of guanandi (Calophyllum brasiliense) fruit and seeds with ripening and harvesting time. The reproductive cycle of a native plant population, located in the coast of the state of Paraná, Brazil, was monitored from anthesis until fruit dispersion, during three years. Monthly harvests were performed, and the morphological and physical aspects (color, size, dry mass, and moisture content) of fruit and embryos were evaluated, as well as the physiological ones (germination and vigor) of seeds. The ripening process of guanandi seeds occurred between 200 and 240 days after anthesis (DAA), with anthesis peak in February and fruit dispersion in October. The maximum dry matter mass accumulation was not synchronized with the maximum physiological potential of the seeds. Germination potential was reached close to the middle of the maturation process, and maximum vigor at the end of the cycle. Fruit external color did not change from 110 DAA onwards. The morphological characteristics related to harvesting time are fruit pulp and embryo weakly adherent to the endocarp and to the tegument, respectively, as well as the dark-brown color of these tissues.
DOI: 10.6084/m9.figshare.6044936
title: Larval development of the Mexican Snook, Centropomus poeyi (Teleostei: Centropomidae)

description: ABSTRACT We document for the first time the early ontogeny of Centropomus poeyi based on captive raised material representing 0-19 days posthatch (dph). The achievement of early developmental landmarks (i.e., yolk-sac depletion, flexion, development of fins) and changes in pigmentation are described (1.4 mm NL-10.6 mm SL; 0-19 dph) and documented for a subset of individuals using high quality photographs. The ontogeny of the viscerocranium is also described (2.4 mm NL-10.6 mm SL; 6-19 dph). Development in C. poeyi occurs over a short period with attainment of the juvenile stage (i.e., full complement of fin rays present in each fin) occurring by 6.9 mm SL. The ontogeny of external pigmentation in C. poeyi is marked by two trends throughout growth: (1) a decrease in pigmentation dorsally; and (2) an increase in pigmentation ventrally along the midline. Development of the viscerocranium begins with the appearance of the maxilla and dentary in individuals of 2.4 mm NL, coinciding with the depletion of the yolk-sac. By 10.6 mm SL all bones of the viscerocranium are present and teeth are present on all teeth-bearing bones of the adult. Aspects of early development in C. poeyi are compared with the congeners C. undecimalis and C. parallelus.
DOI: 10.6084/m9.figshare.6044936.v1
title: Larval development of the Mexican Snook, Centropomus poeyi (Teleostei: Centropomidae)

description: ABSTRACT We document for the first time the early ontogeny of Centropomus poeyi based on captive raised material representing 0-19 days posthatch (dph). The achievement of early developmental landmarks (i.e., yolk-sac depletion, flexion, development of fins) and changes in pigmentation are described (1.4 mm NL-10.6 mm SL; 0-19 dph) and documented for a subset of individuals using high quality photographs. The ontogeny of the viscerocranium is also described (2.4 mm NL-10.6 mm SL; 6-19 dph). Development in C. poeyi occurs over a short period with attainment of the juvenile stage (i.e., full complement of fin rays present in each fin) occurring by 6.9 mm SL. The ontogeny of external pigmentation in C. poeyi is marked by two trends throughout growth: (1) a decrease in pigmentation dorsally; and (2) an increase in pigmentation ventrally along the midline. Development of the viscerocranium begins with the appearance of the maxilla and dentary in individuals of 2.4 mm NL, coinciding with the depletion of the yolk-sac. By 10.6 mm SL all bones of the viscerocranium are present and teeth are present on all teeth-bearing bones of the adult. Aspects of early development in C. poeyi are compared with the congeners C. undecimalis and C. parallelus.
DOI: 10.6084/m9.figshare.6044933
title: Life-history characteristics of Pseudoplatystoma metaense (Teleostei: Siluriformes: Pimelodidae) from the northwestern Orinoco River Basin

description: ABSTRACT The tiger shovelnose catfish (Pseudoplatystoma metaense) identified in literature prior to 2007 as P. tigrinum is the most important catfish species in the Orinoco River fishery, because of its abundance and high market price. The aim of this study was to obtain age, growth and population parameters of the of tiger shovelnose catfish. A total of 2,183 catfish, were sampled during 1996-2003 from the commercial fishery of the northwestern Orinoco River Basin in Venezuela, and were aged by examination of otoliths. Validation of otolith annuli was achieved with marginal increment analysis. Results suggest that, the opaque and translucent zones of otoliths were formed once per year. Length-weight relation and the von Bertalanffy growth model parameters were used to evaluate growth and other population parameters. A strong link between the annual flood pulse and opaque zone formation was confirmed in tiger shovelnose catfish. The total mortality (Z) and natural mortality (M) were estimated. Based in the reference points for optimal and limit fishing mortality rates (F opt and F limit ) this species is exploited above optimum levels. We thus recommend a closed fishing season during their seasonal reproduction, and establishment of a system of fish refuges to help sustain the population.
DOI: 10.6084/m9.figshare.6044933.v1
title: Life-history characteristics of Pseudoplatystoma metaense (Teleostei: Siluriformes: Pimelodidae) from the northwestern Orinoco River Basin

description: ABSTRACT The tiger shovelnose catfish (Pseudoplatystoma metaense) identified in literature prior to 2007 as P. tigrinum is the most important catfish species in the Orinoco River fishery, because of its abundance and high market price. The aim of this study was to obtain age, growth and population parameters of the of tiger shovelnose catfish. A total of 2,183 catfish, were sampled during 1996-2003 from the commercial fishery of the northwestern Orinoco River Basin in Venezuela, and were aged by examination of otoliths. Validation of otolith annuli was achieved with marginal increment analysis. Results suggest that, the opaque and translucent zones of otoliths were formed once per year. Length-weight relation and the von Bertalanffy growth model parameters were used to evaluate growth and other population parameters. A strong link between the annual flood pulse and opaque zone formation was confirmed in tiger shovelnose catfish. The total mortality (Z) and natural mortality (M) were estimated. Based in the reference points for optimal and limit fishing mortality rates (F opt and F limit ) this species is exploited above optimum levels. We thus recommend a closed fishing season during their seasonal reproduction, and establishment of a system of fish refuges to help sustain the population.
DOI: 10.6084/m9.figshare.6044930
title: ESTIMATES OF MONTHLY AND ANNUAL EVAPORATION RATES AND EVAPORATED VOLUMES PER UNIT TIME IN THE TUCURUÍ-PA AND LAJEADO-TO HYDROELECTRIC POWER PLANT RESERVOIRS BASED ON DIFFERENT METHODS

description: ABSTRACT Evaporation rates in reservoirs influence the volume of water available for multiple uses. Thus, the objective of this study was to estimate the evaporation rates and the evaporated volumes per unit time in the Tucuruí-PA and Lajeado-TO reservoirs based on the methods in Linacre (1993), Kohler et al. (1955), Morton (1983), Bruin & Keijman (1979) and Penman (1948) method was adopted as the standard. The mean annual evaporation rates in the Tucuruí and Lajeado reservoirs, estimated by the Penman method, were similar, with values of 1,784 mm and 1,882 mm, respectively. None of the alternative analyzed methods could be used to estimate the mean annual evaporation in the Tucuruí and Lajeado reservoirs and could not replace the Penman method. However, the Linacre (1993) and Bruin & Keijman (1979) methods could be used to estimate monthly evaporation during the dry season in Tucuruí. The mean evaporated volume per unit time and the mean net evaporated volume per unit time in the Tucuruí reservoir correspond to 120% and 50%, respectively, of the total water demand in the Tocantins-Araguaia region, while the mean evaporated volume per unit time and the mean net evaporated volume per unit time in the Lajeado reservoir correspond to 120% and 50%, respectively, of the total water demand in the basin.
DOI: 10.6084/m9.figshare.6044930.v1
title: ESTIMATES OF MONTHLY AND ANNUAL EVAPORATION RATES AND EVAPORATED VOLUMES PER UNIT TIME IN THE TUCURUÍ-PA AND LAJEADO-TO HYDROELECTRIC POWER PLANT RESERVOIRS BASED ON DIFFERENT METHODS

description: ABSTRACT Evaporation rates in reservoirs influence the volume of water available for multiple uses. Thus, the objective of this study was to estimate the evaporation rates and the evaporated volumes per unit time in the Tucuruí-PA and Lajeado-TO reservoirs based on the methods in Linacre (1993), Kohler et al. (1955), Morton (1983), Bruin & Keijman (1979) and Penman (1948) method was adopted as the standard. The mean annual evaporation rates in the Tucuruí and Lajeado reservoirs, estimated by the Penman method, were similar, with values of 1,784 mm and 1,882 mm, respectively. None of the alternative analyzed methods could be used to estimate the mean annual evaporation in the Tucuruí and Lajeado reservoirs and could not replace the Penman method. However, the Linacre (1993) and Bruin & Keijman (1979) methods could be used to estimate monthly evaporation during the dry season in Tucuruí. The mean evaporated volume per unit time and the mean net evaporated volume per unit time in the Tucuruí reservoir correspond to 120% and 50%, respectively, of the total water demand in the Tocantins-Araguaia region, while the mean evaporated volume per unit time and the mean net evaporated volume per unit time in the Lajeado reservoir correspond to 120% and 50%, respectively, of the total water demand in the basin.
DOI: 10.6084/m9.figshare.6044927
title: Fish movement in an Atlantic Forest stream

description: ABSTRACT Given the importance of fish movement to the dynamics and maintenance of stream dwelling fish communities from the Atlantic Forest, we analysed patterns of fish movement in a coastal stream from Southeastern Brazil, using mark-recapture technique. Displacement distance of each species were presented and discussed considering seasonal (rainy and dry) and body size patterns. We marked 10 species along the stream and recaptured 440 (34.6%) of the 1,270 marked fishes. The species with significant number of upstream moving individuals were Astyanax janeiroensis, Characidium interruptum, Astyanax hastatus, Parotocinclus maculicauda and Awaous tajasica. Only Pimelodella lateristriga presented significant differences between resident and moving individuals. Characidium interruptum and A. tajasica demonstrated greater downstream and upstream movement, respectively, moving up to 2,100 m. Even after controlling for species identity we found no significant correlation between fish length and individual displacement distance. Fishes moved longer distances during the rainy season, in accordance to the breeding season. Patterns of fish movement were in agreement to life-history traits of many of the studied species and can be reflecting specific behaviour and morphologies.
DOI: 10.6084/m9.figshare.6044927.v1
title: Fish movement in an Atlantic Forest stream

description: ABSTRACT Given the importance of fish movement to the dynamics and maintenance of stream dwelling fish communities from the Atlantic Forest, we analysed patterns of fish movement in a coastal stream from Southeastern Brazil, using mark-recapture technique. Displacement distance of each species were presented and discussed considering seasonal (rainy and dry) and body size patterns. We marked 10 species along the stream and recaptured 440 (34.6%) of the 1,270 marked fishes. The species with significant number of upstream moving individuals were Astyanax janeiroensis, Characidium interruptum, Astyanax hastatus, Parotocinclus maculicauda and Awaous tajasica. Only Pimelodella lateristriga presented significant differences between resident and moving individuals. Characidium interruptum and A. tajasica demonstrated greater downstream and upstream movement, respectively, moving up to 2,100 m. Even after controlling for species identity we found no significant correlation between fish length and individual displacement distance. Fishes moved longer distances during the rainy season, in accordance to the breeding season. Patterns of fish movement were in agreement to life-history traits of many of the studied species and can be reflecting specific behaviour and morphologies.
DOI: 10.6084/m9.figshare.6044924
title: Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae)

description: ABSTRACT The chromosomal location of 5S rRNA and U2 snRNA genes of Piabina argentea, Piabarchus stramineus and two Bryconamericus species from two different Brazilian river basins were investigated, in order to contribute to the understanding of evolutionary characteristics of these repetitive DNAs in the subfamily Stevardiinae. The diploid chromosome number was 2n = 52 for Bryconamericus cf. iheringii, Bryconamericus turiuba, Piabarchus stramineus and Piabina argentea. The 5S rDNA clusters were located on one chromosome pair in P. stramineus and B. cf. iheringii, and on two pairs in B. turiuba and P. argentea. The U2 snDNA clusters were located on the one pair in all species. Two-color FISH experiments showed that the co-localization between 5S rDNA and U2 snDNA in P. stramineus can represent a marker for this species. Thus, the present study demonstrated that the number of U2 snDNA clusters observed for the four species was conserved, but particular characteristics can be found in the genome of each species.
DOI: 10.6084/m9.figshare.6044924.v1
title: Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae)

description: ABSTRACT The chromosomal location of 5S rRNA and U2 snRNA genes of Piabina argentea, Piabarchus stramineus and two Bryconamericus species from two different Brazilian river basins were investigated, in order to contribute to the understanding of evolutionary characteristics of these repetitive DNAs in the subfamily Stevardiinae. The diploid chromosome number was 2n = 52 for Bryconamericus cf. iheringii, Bryconamericus turiuba, Piabarchus stramineus and Piabina argentea. The 5S rDNA clusters were located on one chromosome pair in P. stramineus and B. cf. iheringii, and on two pairs in B. turiuba and P. argentea. The U2 snDNA clusters were located on the one pair in all species. Two-color FISH experiments showed that the co-localization between 5S rDNA and U2 snDNA in P. stramineus can represent a marker for this species. Thus, the present study demonstrated that the number of U2 snDNA clusters observed for the four species was conserved, but particular characteristics can be found in the genome of each species.