cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6208121.v1
title: FOURIER TRANSFORM INFRARED SPECTROSCOPY, THERMOGRAVIMETRIC ANALYSIS, SCANNING ELECTRON MICROSCOPY AS SUPPORTING TOOLS IN QUALITY CONTROL OF ANTIPARASITICS

description: This study shows that Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) can be used as supporting tools for the evaluation of the quality of antiparasitics. In addition, an analytical methodology was developed and validated to quantify simultaneously thiabendazole (TB), febantel (FB), toltrazuril (TZ), and fluazuron (FZ) in bulk and in their veterinary pharmaceutical formulations using reverse phase high performance liquid chromatography (RP-HPLC). In order to investigate stability, pharmaceuticals were submitted to degradation processes under different conditions, such as recommended by the International Conference on Harmonization. The chromatographic conditions were optimized and the validation parameters, such as selectivity, linearity, detection limit, quantification limit, precision, accuracy, and robustness showed results within acceptable standards. All analytes were stable in the stability assays in acid and basic media and thermal conditions, except in the oxidation process, which presented two degradation peaks. Physicochemical characterization by TGA, FTIR, and SEM of raw materials of TB, FB, TZ, and FZ provided information about the authenticity of the analytes, proving the wide applicability of the instrumental techniques. The RP-HPLC proposed method was found to be accurate, precise, and reproducible and can in addition be used for routine quality control analysis.
DOI: 10.6084/m9.figshare.6208118
title: THE LEARNING OF SUBJUNCTIVE PRESENT TENSE OF SPANISH IN TEMPORAL SENTENCES WITH CUANDO BY BRAZILIAN COLLEGE STUDENTS: THE EFFECTS OF INSTRUCTIONAL INTERVENTION IN LANGUAGE TRANSFER REDUCTION

description: ABSTRACT: In this study, we analyze and discuss the role of the instructional intervention in the reduction of the non-application of the subjunctive present tense of Spanish in temporal sentences with cuando by Brazilian college students. Tasks as oral and written production were used to obtain data, which were submitted to Standard Normal Test. Twelve students from a private university participated in this study. In a short term, the results suggested the reduction of the alternative forms. These reductions have kept stable in medium term, that is, there was a greater application of cases of language transfer in the pre-test and a reduction of its application in the post-instructional phases. Thus, starting from an inversely proportional relation, the pedagogical treatment had provided a durable effect on the learning of the subjunctive present tense in temporal sentences with cuando. We add to this the fact that the reduction of language transfer also can be useful as favorable evidence of the instructional benefits.
DOI: 10.6084/m9.figshare.6208118.v1
title: THE LEARNING OF SUBJUNCTIVE PRESENT TENSE OF SPANISH IN TEMPORAL SENTENCES WITH CUANDO BY BRAZILIAN COLLEGE STUDENTS: THE EFFECTS OF INSTRUCTIONAL INTERVENTION IN LANGUAGE TRANSFER REDUCTION

description: ABSTRACT: In this study, we analyze and discuss the role of the instructional intervention in the reduction of the non-application of the subjunctive present tense of Spanish in temporal sentences with cuando by Brazilian college students. Tasks as oral and written production were used to obtain data, which were submitted to Standard Normal Test. Twelve students from a private university participated in this study. In a short term, the results suggested the reduction of the alternative forms. These reductions have kept stable in medium term, that is, there was a greater application of cases of language transfer in the pre-test and a reduction of its application in the post-instructional phases. Thus, starting from an inversely proportional relation, the pedagogical treatment had provided a durable effect on the learning of the subjunctive present tense in temporal sentences with cuando. We add to this the fact that the reduction of language transfer also can be useful as favorable evidence of the instructional benefits.
DOI: 10.6084/m9.figshare.6208115
title: Evaluation of strategic control of caprine arthritis- encephalitis in dairy herd goats

description: ABSTRACT The aim of this study was to evaluate the periodic use of more sensitive diagnostic tests associated to management practices for the effective control of caprine arthritis-encephalitis (CAE). We carried out eight blood samples in does and bucks. From the first to the seventh analysis, the samples were quarterly, using Agarose Gel Immunodiffusion (AGID), Enzyme linked immunosorbent assay (i-ELISA) and Western Blot (WB) tests. The eighth collection was made six months after the seventh, using the WB and Polymerase Chain Reaction (PCR). The prevalence of CAE was 6.8%, 14.9% and 39.2% in the AGID, i-ELISA and WB respectively. The last analysis detected 0.9% of animals positive by WB and 10.8% by PCR. Although they do not eradicate CAE, steps taken together with the periodic use of serological tests and the combination with PCR were important to significantly reduce positive animals in the herd.
DOI: 10.6084/m9.figshare.6208115.v1
title: Evaluation of strategic control of caprine arthritis- encephalitis in dairy herd goats

description: ABSTRACT The aim of this study was to evaluate the periodic use of more sensitive diagnostic tests associated to management practices for the effective control of caprine arthritis-encephalitis (CAE). We carried out eight blood samples in does and bucks. From the first to the seventh analysis, the samples were quarterly, using Agarose Gel Immunodiffusion (AGID), Enzyme linked immunosorbent assay (i-ELISA) and Western Blot (WB) tests. The eighth collection was made six months after the seventh, using the WB and Polymerase Chain Reaction (PCR). The prevalence of CAE was 6.8%, 14.9% and 39.2% in the AGID, i-ELISA and WB respectively. The last analysis detected 0.9% of animals positive by WB and 10.8% by PCR. Although they do not eradicate CAE, steps taken together with the periodic use of serological tests and the combination with PCR were important to significantly reduce positive animals in the herd.
DOI: 10.6084/m9.figshare.6208112
title: Fish oil associated to ascorbic acid in diluter for cryopreservation goat semen

description: ABSTRACT The study aimed to evaluate the effect of fish oil inclusion associated with ascorbic acid in the thinner for goat semen cryopreservation. Two male Boers underwent semen collection through the artificial vagina method, ejaculates being then assessed for physical and morphological aspects. After evaluation, a pool was formed, followed by the split into five groups: G1 - yolk-citrate extender and G2, G3, G4 and G5 - yolk-citrate extender plus 1.0; 2.0; 3.0 and 4.0% fish oil and 0.05% ascorbic acid, respectively. After thawing, physical evaluations of semen were assessed and additional testing slow heat resistance (TTR), hiposmotic (HO), acrosome integrity and compression of sperm chromatin. There was linear increase (P&lt;0.05) post-thaw motility. No difference was obtained for post-thaw vigor and there was no influence of the association of fish oil and ascorbic acid in TTR. Plasma membrane integrity, by hyposmotic test (HO), presented a mean of reactive spermatozoa of 23.5±5.96% (P&gt;0.05). There was linear increase for intact acrosome and decreasing acrosome irregular (P&lt;0.05). In the analysis of the chromatin compaction, approximately 3% of damages (P&gt;0.05) were observed. The inclusion of 4% fish oil plus ascorbic acid in diluter improved motility and acrosome integrity after cryopreservation.
DOI: 10.6084/m9.figshare.6208112.v1
title: Fish oil associated to ascorbic acid in diluter for cryopreservation goat semen

description: ABSTRACT The study aimed to evaluate the effect of fish oil inclusion associated with ascorbic acid in the thinner for goat semen cryopreservation. Two male Boers underwent semen collection through the artificial vagina method, ejaculates being then assessed for physical and morphological aspects. After evaluation, a pool was formed, followed by the split into five groups: G1 - yolk-citrate extender and G2, G3, G4 and G5 - yolk-citrate extender plus 1.0; 2.0; 3.0 and 4.0% fish oil and 0.05% ascorbic acid, respectively. After thawing, physical evaluations of semen were assessed and additional testing slow heat resistance (TTR), hiposmotic (HO), acrosome integrity and compression of sperm chromatin. There was linear increase (P&lt;0.05) post-thaw motility. No difference was obtained for post-thaw vigor and there was no influence of the association of fish oil and ascorbic acid in TTR. Plasma membrane integrity, by hyposmotic test (HO), presented a mean of reactive spermatozoa of 23.5±5.96% (P&gt;0.05). There was linear increase for intact acrosome and decreasing acrosome irregular (P&lt;0.05). In the analysis of the chromatin compaction, approximately 3% of damages (P&gt;0.05) were observed. The inclusion of 4% fish oil plus ascorbic acid in diluter improved motility and acrosome integrity after cryopreservation.
DOI: 10.6084/m9.figshare.6208109
title: The intravenous polyionic solution with 84mEq/L of lactate infusion rate determines the intensity of the alkalizing effect in horses

description: ABSTRACT The aim of this study was to investigate the effects of an intravenous polyionic solution containing 84mEq/L of lactate (L84) on the hydroelectrolyte and acid-base balances when administered quickly or slowly in horses. Five healthy adult horses received the L84 solution, in a volume corresponding to 10% of BW, by continuous intravenous infusion, in two instants: a) rapid infusion (16.66mL/kg/h) during 6 hours; b) slow infusion (8.33mL/kg/h) during 12 hours. Venous blood samples were taken at the beginning of the infusion (hour 0) and 3, 6, 9, 12, and 24 hours after. Urine samples were taken at 0, 6, 12, and 24h. pH (blood and urine), pCO2, HCO3-, BE, TPP, L-lactate, Na+, K+, Cl-, AG, SID, Atot, PVV, urine specific gravity, and L-lactate, Na+, K+, and Cl- renal fractional clearance were determined. The L84 solution causes lower magnitude alkalizing effect when administered slowly, due to the gradual correction of the iatrogenic imbalance by the kidneys during the infusion period. The L84 solution infused at a low rate in horses could be recommended in cases where metabolic acidosis is suspected and it is not possible to quantify the imbalance degree.
DOI: 10.6084/m9.figshare.6208109.v1
title: The intravenous polyionic solution with 84mEq/L of lactate infusion rate determines the intensity of the alkalizing effect in horses

description: ABSTRACT The aim of this study was to investigate the effects of an intravenous polyionic solution containing 84mEq/L of lactate (L84) on the hydroelectrolyte and acid-base balances when administered quickly or slowly in horses. Five healthy adult horses received the L84 solution, in a volume corresponding to 10% of BW, by continuous intravenous infusion, in two instants: a) rapid infusion (16.66mL/kg/h) during 6 hours; b) slow infusion (8.33mL/kg/h) during 12 hours. Venous blood samples were taken at the beginning of the infusion (hour 0) and 3, 6, 9, 12, and 24 hours after. Urine samples were taken at 0, 6, 12, and 24h. pH (blood and urine), pCO2, HCO3-, BE, TPP, L-lactate, Na+, K+, Cl-, AG, SID, Atot, PVV, urine specific gravity, and L-lactate, Na+, K+, and Cl- renal fractional clearance were determined. The L84 solution causes lower magnitude alkalizing effect when administered slowly, due to the gradual correction of the iatrogenic imbalance by the kidneys during the infusion period. The L84 solution infused at a low rate in horses could be recommended in cases where metabolic acidosis is suspected and it is not possible to quantify the imbalance degree.
DOI: 10.6084/m9.figshare.6208100
title: Seroepidemiology of Neospora caninum among goats (Capra hircus) in the state of Paraíba, northeastern Brazil

description: ABSTRACT Although goat dairy farms in Brazil may have a higher risk of infection by Neospora caninum than beef farms, risk factor evaluation on a representative population remains to be fully established in Brazil. Accordingly, this study aimed to establish the occurrence of anti-N. caninum antibodies and factors associated with exposure in 406 blood samples from five dairy and three beef goat farms in the state of Paraíba, northeastern Brazil. Anti-N. caninum antibodies were detected by indirect immunofluorescence assay (IFA), with samples considered positive when reacting with dilution ≥ 1:50. A total of 106/406 goats (26.11%; 95% CI: 21.96-30.72%) were seroreactive comprising 2/61 (3.28%), 10/45 (22.22%), 13/50 (26.00%), 17/51 (33.33%) to 29/46 (63.04%) in dairy farms, and from 3/54 (5.56%), 12/50 (24.00%) to 20/49 (40.82%) on the beef farms. No significant associations were found in relation to age, gender, dairy versus beef farms, occurrence of abortions or mummified fetuses, and seroreactivity to N. caninum (P&gt;0.05). In conclusion, goat farms in the state of Paraíba showed the highest occurrence of anti-N. caninum antibodies to date in Brazil.
DOI: 10.6084/m9.figshare.6208100.v1
title: Seroepidemiology of Neospora caninum among goats (Capra hircus) in the state of Paraíba, northeastern Brazil

description: ABSTRACT Although goat dairy farms in Brazil may have a higher risk of infection by Neospora caninum than beef farms, risk factor evaluation on a representative population remains to be fully established in Brazil. Accordingly, this study aimed to establish the occurrence of anti-N. caninum antibodies and factors associated with exposure in 406 blood samples from five dairy and three beef goat farms in the state of Paraíba, northeastern Brazil. Anti-N. caninum antibodies were detected by indirect immunofluorescence assay (IFA), with samples considered positive when reacting with dilution ≥ 1:50. A total of 106/406 goats (26.11%; 95% CI: 21.96-30.72%) were seroreactive comprising 2/61 (3.28%), 10/45 (22.22%), 13/50 (26.00%), 17/51 (33.33%) to 29/46 (63.04%) in dairy farms, and from 3/54 (5.56%), 12/50 (24.00%) to 20/49 (40.82%) on the beef farms. No significant associations were found in relation to age, gender, dairy versus beef farms, occurrence of abortions or mummified fetuses, and seroreactivity to N. caninum (P&gt;0.05). In conclusion, goat farms in the state of Paraíba showed the highest occurrence of anti-N. caninum antibodies to date in Brazil.
DOI: 10.6084/m9.figshare.6208097
title: Influence of nutritional status, laboratory parameters and dietary patterns upon urinary acid excretion in calcium stone formers.

description: ABSTRACT Introduction: Obesity and Metabolic Syndrome (MS) are associated with low urinary pH and represent risk factors for nephrolithiasis, especially composed by uric acid. Acidogenic diets may also contribute to a reduction of urinary pH. Propensity for calcium oxalate precipitation has been shown to be higher with increasing features of the MS. Objective: A retrospective evaluation of anthropometric and body composition parameters, MS criteria and the dietary patterns of overweight and obese calcium stone formers and their impact upon urinary pH and other lithogenic parameters was performed. Methods: Data regarding anthropometry, body composition, serum and urinary parameters and 3-days dietary records were obtained from medical records of 102(34M/68F) calcium stone formers. Results: A negative correlation was found between urinary pH, waist circumference and serum uric acid levels (males). The endogenous production of organic acids (OA) was positively correlated with triglycerides levels and number of features of MS (males), and with glucose, uric acid and triglycerides serum levels, and number of features of MS (females). No significant correlations were detected between Net Acid Excretion (NAE) or Potential Renal Acid Load of the diet with any of the assessed parameters. A multivariate analysis showed a negative association between OA and urinary pH. Conclusion: The endogenous production of OA and not an acidogenic diet were found to be independently predictive factors for lower urinary pH levels in calcium stone formers. Hypercalciuric and/or hyperuricosuric patients presented higher OA levels and lower levels of urinary pH.
DOI: 10.6084/m9.figshare.6208097.v1
title: Influence of nutritional status, laboratory parameters and dietary patterns upon urinary acid excretion in calcium stone formers.

description: ABSTRACT Introduction: Obesity and Metabolic Syndrome (MS) are associated with low urinary pH and represent risk factors for nephrolithiasis, especially composed by uric acid. Acidogenic diets may also contribute to a reduction of urinary pH. Propensity for calcium oxalate precipitation has been shown to be higher with increasing features of the MS. Objective: A retrospective evaluation of anthropometric and body composition parameters, MS criteria and the dietary patterns of overweight and obese calcium stone formers and their impact upon urinary pH and other lithogenic parameters was performed. Methods: Data regarding anthropometry, body composition, serum and urinary parameters and 3-days dietary records were obtained from medical records of 102(34M/68F) calcium stone formers. Results: A negative correlation was found between urinary pH, waist circumference and serum uric acid levels (males). The endogenous production of organic acids (OA) was positively correlated with triglycerides levels and number of features of MS (males), and with glucose, uric acid and triglycerides serum levels, and number of features of MS (females). No significant correlations were detected between Net Acid Excretion (NAE) or Potential Renal Acid Load of the diet with any of the assessed parameters. A multivariate analysis showed a negative association between OA and urinary pH. Conclusion: The endogenous production of OA and not an acidogenic diet were found to be independently predictive factors for lower urinary pH levels in calcium stone formers. Hypercalciuric and/or hyperuricosuric patients presented higher OA levels and lower levels of urinary pH.
DOI: 10.6084/m9.figshare.6208094
title: Impact evaluation caused by disponibility of free and complexed 17β-estradiol into cyclodextrin in the aquatic environment in tilapia (Oreochromis niloticus)

description: ABSTRACT Toxic effects of free and complexed 17β-estradiol (E2) hormone into β-cyclodextrin (CD) on the behavior and physiology of tilapia (Oreochromis niloticus) were evaluated. The fish were observed for 30 days in two stages of development (fingerling and juvenile) by the ad libitum method to make an ethogram. After this, juveniles were divided into three groups: control and exposed to free E2 (10ng/L) and complexed into β-cyclodextrin (β-CD:E2) for 90 days. The behavior was evaluated through scan sampling method, feed intake, body mass and mortality at different intervals. The fingerlings and juveniles showed behavioral patterns with different display frequencies (P&lt;0.05) for events: Move Away (4.7±1.3 and 3.6±0.6%) and Waving Repulsion (2.3±0.9 and 1.3±1.0%). The juveniles exposed to β-CD:E2 complex showed a significant increase (P&lt;0.05) in the frequency of display of aggressive behaviors as Move Away, Caudal Attack, Clash Extended, Chase, Escape and decrease of mortality when compared to group exposed to free E2 and control. In conclusion, complexation of E2 into β-CD modified E2 toxicity, because it promoted an increase in the frequency of display of aggressive behaviors and it affected the mortality of animals.
DOI: 10.6084/m9.figshare.6208094.v1
title: Impact evaluation caused by disponibility of free and complexed 17β-estradiol into cyclodextrin in the aquatic environment in tilapia (Oreochromis niloticus)

description: ABSTRACT Toxic effects of free and complexed 17β-estradiol (E2) hormone into β-cyclodextrin (CD) on the behavior and physiology of tilapia (Oreochromis niloticus) were evaluated. The fish were observed for 30 days in two stages of development (fingerling and juvenile) by the ad libitum method to make an ethogram. After this, juveniles were divided into three groups: control and exposed to free E2 (10ng/L) and complexed into β-cyclodextrin (β-CD:E2) for 90 days. The behavior was evaluated through scan sampling method, feed intake, body mass and mortality at different intervals. The fingerlings and juveniles showed behavioral patterns with different display frequencies (P&lt;0.05) for events: Move Away (4.7±1.3 and 3.6±0.6%) and Waving Repulsion (2.3±0.9 and 1.3±1.0%). The juveniles exposed to β-CD:E2 complex showed a significant increase (P&lt;0.05) in the frequency of display of aggressive behaviors as Move Away, Caudal Attack, Clash Extended, Chase, Escape and decrease of mortality when compared to group exposed to free E2 and control. In conclusion, complexation of E2 into β-CD modified E2 toxicity, because it promoted an increase in the frequency of display of aggressive behaviors and it affected the mortality of animals.
DOI: 10.6084/m9.figshare.6208091
title: Inoculation methods and aggressiveness of five Fusarium species against peach palm

description: ABSTRACT: Fusarium wilt is a major disease which affects peach palm (Bactris gasipaes Kunth.var gasipaes Henderson). This study aimed to evaluate inoculation methods and aggressiveness of isolates of five Fusarium species on peach palm. Fusarium proliferatum can infect the leaves, stem, and roots of peach palm. F. proliferatum, F. oxysporum species complex (FOSC), F. verticillioides, F. solani species complex (FSSC), and Gibberella fujikuroi species complex (GFSC) are pathogenic to peach palm. The use of Fusarium-colonized ground corn for root inoculation was effective and reduced the level of damage to plants.
DOI: 10.6084/m9.figshare.6208091.v1
title: Inoculation methods and aggressiveness of five Fusarium species against peach palm

description: ABSTRACT: Fusarium wilt is a major disease which affects peach palm (Bactris gasipaes Kunth.var gasipaes Henderson). This study aimed to evaluate inoculation methods and aggressiveness of isolates of five Fusarium species on peach palm. Fusarium proliferatum can infect the leaves, stem, and roots of peach palm. F. proliferatum, F. oxysporum species complex (FOSC), F. verticillioides, F. solani species complex (FSSC), and Gibberella fujikuroi species complex (GFSC) are pathogenic to peach palm. The use of Fusarium-colonized ground corn for root inoculation was effective and reduced the level of damage to plants.
DOI: 10.6084/m9.figshare.6208088
title: The Gene Ontology Differs in Bursa of Fabricius Between Two Breeds of Ducks Post Hatching by Enriching the Differentially Expressed Genes

description: ABSTRACT The bursa of Fabricius (BF) is the central humoral immune organ unique to birds. The present study investigated the possible difference on a molecular level between two duck breeds. The digital gene expression profiling (DGE) technology was used to enrich the differentially expressed genes (DEGs) in BF between the Jianchang and Nonghua-P strains of ducks. DGE data identified 195 DEGs in the bursa. Gene Ontology (GO) analysis suggested that DEGs were mainly enriched in the metabolic pathways and ribosome components. Pathways analysis identified the spliceosome, RNA transport, RNA degradation process, Jak-STAT signaling pathway, TNF signaling pathway and B cell receptor signaling pathway. The results indicated that the main difference in the BF between the two duck strains was in the capabilities of protein formation and B cell development. These data have revealed the main divergence in the BF on a molecular level between genetically different duck breeds and may help to perform molecular breeding programs in poultry in the future.