DOI: 10.5061/dryad.34tmpg4sw
title: Plant growth-promoting rhizobacteria modulate induced corn defense against Spodoptera litura (Lepidoptera: Noctuidae)

description: Common cutworm, Spodoptera litura is an important pest of corn causing
 significant crop yield loss. Synthetic insecticides have mostly been used
 to combat this pest, raising human and environmental health concerns.
 Plant-growth promoting rhizobacteria (PGPR) could compensate for or
 augment the harmful effects of agrochemicals. Herein, we aimed to assess
 whether PGPR-induced defenses in corn plants impact the host-plant
 selection behavior of S. litura. Headspace volatile organic compounds
 (VOCs) were analyzed using Gas chromatography-mass spectrometry (GC-MS).
 Larvae-fed inoculated corn exhibited lower weights and RGR than
 non-inoculated plants. Under choice experiments, PGPR-treated plants
 significantly reduced percentage leaf damage area and oviposition rate
 compared to untreated plants. VOC ratio emission varied significantly
 between control and PGPR treatments, which, in part, explains feeding and
 oviposition deterrence in PGPR-treated plants. The results demonstrate
 that PGPR inoculation can enhance corn resistance to S. litura,
 making it a promising candidate for crop protection strategies.
description: See the Manuscript: Cortez Jr., Amado et al. (2024), Plant
 growth-promoting rhizobacteria modulate induced corn defense against
 Spodoptera litura (Lepidoptera: Noctuidae).<br>All datasets were
 analyzed using the SPSS statistical software package, 2011 version 20.0,
 IBM Corp., Armonk, NY.
description: This CORTEZ_2024_PGPR_SPODOPTERA_LITURA_DATA_readme.txt file was generated
 on 2024-05-17 by AMADO O. CORTEZ, JR. GENERAL INFORMATION 1. Title of
 Dataset: Plant growth-promoting rhizobacteria modulate induced corn
 defense against Spodoptera litura (Lepidoptera: Noctuidae) 2. Author
 Information A. Principal Investigator Contact Information Name: Amado O.
 Cortez, Jr. Institution: National Chung Hsing University Address: 145
 Xingda Road, South District, Taichung City 402, Taiwan Email:
 [cortezamadojr@gmail.com](mailto:cortezamadojr@gmail.com) B. Associate or
 Co-investigator Contact Information Name: Naoko Yoshinaga Institution:
 Kyoto University Address: Yoshidahonmachi, Sakyo Ward, Kyoto, 606-8501,
 Japan Email:
 [yoshinaga.naoko.5v@kyoto-u.ac.jp](mailto:yoshinaga.naoko.5v@kyoto-u.ac.jp) C. Alternate Contact Information Name: Dr. Shaw-Yhi Hwang Institution: National Chung Hsing University Address: 145 Xingda Road, South District, Taichung City 402, Taiwan Email: [oleander@dragon.nchu.edu.tw](mailto:oleander@dragon.nchu.edu.tw) 3. Date of data collection (range) Jul 2022 to February 2023 4. Geographic location of data collection Chemical Ecology Laboratory, Department of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan 5. Information about funding sources that supported the collection of the data: N/A SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: N/A 2. Links to publications that cite or use the data: Publication in the Journal of Bioscience, Biotechnology, and Biochemistry 3. Links to other publicly accessible locations of the data: N/A 4. Links/relationships to ancillary data sets: N/A 5. Was data derived from another source? No 6. Recommended citation for this dataset: Cortez Jr., Amado et al. (2024), Plant growth-promoting rhizobacteria modulate induced corn defense against Spodoptera litura (Lepidoptera: Noctuidae) DATA & FILE OVERVIEW 1. File List: * PGPR_Spodoptera litura_dataset_VOC.CSV UTF-8: Data for Table 1 in the corresponding manuscript. The dataset includes the relative percentile of volatile compounds detected in the PGPR-inoculated and non-inoculated corn plants analyzed using GC-MS. * PGPR_Spodoptera litura_dataset_larval_survival_weight.CSV UTF-8: Data for Figure 1 in the corresponding manuscript. The dataset includes survival percentage and weight of S. litura larvae after feeding on PGPR-treated and untreated corn leaves. * PGPR_Spodoptera litura_dataset_larval_RGR.CSV UTF-8: Data for Figure 2 in the corresponding manuscript. The dataset includes the relative growth rate of S. litura fed with PGPR-treated and untreated corn leaves after 18 days of rearing. * PGPR_Spodoptera litura_dataset_larval_feeding_preference.CSV UTF-8: Data for Figures 3 in the corresponding manuscript. The dataset includes the feeding preference (choice and no-choice test) of S. litura larvae reared on PGPR-treated and untreated corn leaves. * PGPR_Spodoptera litura_dataset_leaf_C/N_contents.CSV UTF-8: Data for Figure 4 in the corresponding manuscript.The dataset includes the leaf carbon and nitrogen contents of PGPR-treated and untreated corn plants. * PGPR_Spodoptera litura_dataset_female_oviposition_preference.CSV UTF-8: Data for Figure 5 in the corresponding manuscript. The dataset includes the oviposition preference (choice and no-choice tests) of female S. litura adults reared on PGPR-treated and untreated corn plants. 1. Relationship between files, if important: N/A 2. Additional related data collected that was not included in the current data package: N/A 3. Are there multiple versions of the dataset? No METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: See the Manuscript: Cortez Jr., Amado et al. (2024), Plant growth-promoting rhizobacteria modulate induced corn defense against Spodoptera litura (Lepidoptera: Noctuidae) 2. Methods for processing the data: See the Manuscript: Cortez Jr., Amado et al. (2024), Plant growth-promoting rhizobacteria modulate induced corn defense against Spodoptera litura (Lepidoptera: Noctuidae) 3. Instrument- or software-specific information needed to interpret the data: The datasets were interpreted using the SPSS statistical software package version 20.0 (IBM Corp., Armonk, NY) and analyzed following the Analysis of Variance (ANOVA) and a non-parametric Kruskal-Wallis rank sums test method. 4. Standards and calibration information, if appropriate: N/A 5. Environmental/experimental conditions: N/A 6. Describe any quality-assurance procedures performed on the data: N/A 7. People involved with sample collection, processing, analysis and/or submission: Amado O. Cortez, Jr., Naoko Yoshinaga, Naoki Mori, and Shaw-Yhi Hwang DATA-SPECIFIC INFORMATION: 1. Number of variables: * PGPR_Spodoptera litura_dataset_VOC.CSV UTF-8: 1 (independent variable), 10 (dependent variables) * PGPR_Spodoptera litura_dataset_larval_survival.CSV UTF-8: 1 (independent variable), 1 (dependent variable) * PGPR_Spodoptera litura_dataset_larval_weight.CSV UTF-8: 1 (independent variable), 1 (dependent variable) * PGPR_Spodoptera litura_dataset_larval_RGR_18_days-rearing.CSV UTF-8: 1 (independent variable), 1 (dependent variable) * PGPR_Spodoptera litura_dataset_larval_RGR_5_days-rearing.CSV UTF-8: 1 (independent variable), 1 (dependent variable) * PGPR_Spodoptera litura_dataset_larval_feeding_preference.CSV UTF-8: 1 (independent variable), 2 (dependent variables) * PGPR_Spodoptera litura_dataset_leaf_C/N_contents.CSV UTF-8: 1 (independent variables), 3 (dependent variables) * PGPR_Spodoptera litura_dataset_female_oviposition_preference.CSV UTF-8: 1 (independent variable), 2 (dependent variables) 1. Number of cases/rows: * PGPR_Spodoptera litura_dataset_VOC.CSV UTF-8: 16 * PGPR_Spodoptera litura_dataset_larval_survival.CSV UTF-8: 16 * PGPR_Spodoptera litura_dataset_larval_weight.CSV UTF-8: 31 * PGPR_Spodoptera litura_dataset_larval_RGR_18_days-rearing.CSV UTF-8: 16 * PGPR_Spodoptera litura_dataset_larval_RGR_5_days-rearing.CSV UTF-8: 31 * PGPR_Spodoptera litura_dataset_larval_feeding_preference.CSV UTF-8: 17 * PGPR_Spodoptera litura_dataset_leaf_C/N_contents.CSV UTF-8: 17 * PGPR_Spodoptera litura_dataset_female_oviposition_preference.CSV UTF-8: 17 1. Variable List: * PGPR_Spodoptera litura_dataset_VOC.CSV UTF-8: PGPR (Independent variable), Sulcatone, Octanal, Decane, Linalool, Nonanal, Cyclohexanol, Decanal, Caryophyllene, Geranylacetone, Nerolidol (dependent variables) * PGPR_Spodoptera litura_dataset_larval_survival.CSV UTF-8: PGPR (Independent variable), larval survival percentage (dependent variables) * PGPR_Spodoptera litura_dataset_larval_weight. CSV UTF-8: PGPR (Independent variable), larval weight (dependent variable) * PGPR_Spodoptera litura_dataset_larval_RGR_days-rearing.CSV UTF-8: PGPR (Independent variables), relative growth rate of larvae (Dependent variable) * PGPR_Spodoptera litura_dataset_larval_feeding_preference.CSV UTF-8: PGPR (Independent variables), Percentage leaf damage area (choice and no-choice test) (Dependent variables) * PGPR_Spodoptera litura_dataset_leaf_C/N_contents.CSV UTF-8: PGPR (Independent variables), Carbon rate, Nitrogen rate, C/N ratio (Dependent variables) * PGPR_Spodoptera litura_dataset_female_oviposition_preference.CSV UTF-8: PGPR (Independent variables), Number of eggs laid (choice and no-choice test) (Dependent variables) 1. Missing data codes: N/A 2. Specialized formats or other abbreviations used: PGPR: Plant-growth promoting rhizobacteria, VOC: Volatile Organic Compounds, C/N: Carbon/Nitrogen