cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.35077/age-dip
title: Taste_Fly_1: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_1: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_1', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C1_20201028_11D02_DBD_VT02378_AD_MCFO_1_to_2_days_20x_brain_2', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,red', displayColor:'(1.0, 0.0, 0.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.6084/m9.figshare.23753280
title: Supplementary Material for: Challenges in Pharmacovigilance: Variability in the Criteria for Determining Drug Associated Acute Kidney Injury (D-AKI) in Retrospective, Observational Studies

description: Drug associated acute kidney injury (D-AKI) accounts for 19 to 26% of acute kidney injury (AKI) events in hospitalized patients and results in outcomes similar to patients with AKI from other etiologies. Diagnosing D-AKI is complex and various criteria have been used. Summary To highlight the variability in D-AKI determination, a review was conducted between January 2017 and December 2022 using PubMed. Search terms included adaptations of “drug associated kidney injury” to identify a sampling of literature discussing definitions and criteria for D-AKI evaluation. The search yielded 291 articles that were uploaded to Rayyan, a software tool used to screen and select studies. Retrospective, observational electronic health record (EHR) studies conducted in hospitalized patients were included. The final sample contained 16 studies for data extraction, representing mostly adult populations (n=13, 81.3%) in non-critical or unspecified inpatient settings (n=12, 75%). Nine studies utilized the recommended Kidney Disease: Improving Global Outcome guidelines (KDIGO) criteria to define AKI. Baseline creatinine criteria for kidney function was provided in 10 studies (62.5%). Eleven studies (68.8%) established a temporal sequence assessment linking nephrotoxin drug exposure to an AKI event, but these criteria were inconsistent among studies using time frames as soon as three months prior to AKI. Conclusion This review highlights the substantial variability in D-AKI criteria in select studies. Minimum expectations about what should be reported and criteria for the elements reported are needed to assure transparency, consistency, and standardization of pharmacovigilance strategies.
DOI: 10.35077/age-dog
title: Taste_Fly_2: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_2: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_2', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C3_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_6', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,blue', displayColor:'(0.0, 0.0, 1.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-dry
title: Taste_Fly_3: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_3: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_3', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C1_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_4', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,red', displayColor:'(1.0, 0.0, 0.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-dub
title: Taste_Fly_4: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_4: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_4', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C1_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_3', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,red', displayColor:'(1.0, 0.0, 0.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-dud
title: Taste_Fly_5: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_5: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_5', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C1_20201028_11D02_DBD_VT02378_AD_MCFO_1_to_2_days_20x_brain_1', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,red', displayColor:'(1.0, 0.0, 0.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-dug
title: Taste_Fly_6: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_6: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_6', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C3_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_5', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,blue', displayColor:'(0.0, 0.0, 1.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-ear
title: Taste_Fly_7: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_7: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_7', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C3_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_1', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,blue', displayColor:'(0.0, 0.0, 1.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-eat
title: Taste_Fly_8: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_8: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_8', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C3_20201028_11D02_DBD_VT02378_AD_MCFO_1_to_2_days_20x_brain_1', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,blue', displayColor:'(0.0, 0.0, 1.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'
DOI: 10.35077/age-eel
title: Taste_Fly_9: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)

description: Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
description: A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
description: DATASET METADATA: title:'Taste_Fly_9: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)', GeneralModality:'other', Technique:'other', Other:'Stochastic labelling' SPECIMEN METADATA: LocalID:'Taste_Fly_9', Species:'Fruit fly', NCBITaxonomy:'NCBI:txid7227', Age:'4 to 6', Ageunit:'Days', Sex:'Male', Genotype:'w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1)', OrganName:'Brain', SampleLocalID:'C1_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_2', Locations:'Subesophageal zone (SEZ)' INSTRUMENT METADATA: MicroscopeType:'Confocal', MicroscopeManufacturerAndModel:'Zeiss LSM 800 Axio Imager.Z2', ObjectiveName:'Zeiss 20x objective (Plan-Apochromat 20x/0.8)', ObjectiveNA:'0.8', ObjectiveMagnification:'20x' IMAGE METADATA: xAxis:'left-to-right', yAxis:'left-to-right', zAxis:'left-to-right', Number:'1,red', displayColor:'(1.0, 0.0, 0.0)', stepSizeX:'0.6239', stepSizeY:'0.6239'