cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/sdvxr-21s77
title: H. Neapolitanus Carboxysomes

description: Carboxysomes purified from H. Neapolitanus, WT, high resolution
description: <strong>Tilt Series Date:</strong> 2019-08-21</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Halothiobacillus neapolitanus</p>
<strong>Strain:</strong> c2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, novaCTF, custom bash and Matlab scripts</p>
<strong>Collaborators and Roles:</strong> purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by the Savage lab</p>
<strong>Sample Preparation:</strong> protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-11/rawdata/</p>11_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 3.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-11/rawdata/11_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        11_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 442.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-11/3dimage_113033/11_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 12.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-11/file_26591/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-11/file_26590/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        TS_11.mrc.mdoc,  30.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-11/rawdata/TS_11.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y4g4p-bq242
title: H. Neapolitanus Carboxysomes

description: Carboxysomes purified from H. Neapolitanus, WT, high resolution
description: <strong>Tilt Series Date:</strong> 2019-08-21</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Halothiobacillus neapolitanus</p>
<strong>Strain:</strong> c2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, novaCTF, custom bash and Matlab scripts</p>
<strong>Collaborators and Roles:</strong> purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by the Savage lab</p>
<strong>Sample Preparation:</strong> protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-58/rawdata/</p>58_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 4.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-58/rawdata/58_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        58_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 286.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-58/3dimage_113127/58_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_58.mrc.mdoc,  30.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-58/rawdata/TS_58.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-58/file_26731/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 16.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-58/file_26732/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/axja9-9f505
title: Shewanella OMV delMTR

description: Shewanella outer membrane vesicles, delMTR (cytochromes) knockout, high resolution
description: <strong>Tilt Series Date:</strong> 2018-09-12</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 82000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Lori Zacharoff in the El Naggar lab at USC; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Sample Preparation:</strong> C-Flat 2/2-300 mesh grid, 10nm BSA-gold, 100% humidity, -1.5 to -5 micron defocus</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-7/rawdata/</p>23_dose-filt.st,  Tilt Series (Pixel Size 0.1778 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-7/rawdata/23_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        23_dose-filt.rec,  Reconstruction (Pixel Size 0.7112 nm), 129.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-7/3dimage_102887/23_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 7.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-7/file_20299/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        TS_23.mrc.mdoc,  24.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-7/rawdata/TS_23.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-7/file_20298/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hntyy-2z105
title: Shewanella OMV delMTR

description: Shewanella outer membrane vesicles, delMTR (cytochromes) knockout, high resolution
description: <strong>Tilt Series Date:</strong> 2018-09-12</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 82000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Lori Zacharoff in the El Naggar lab at USC; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Sample Preparation:</strong> C-Flat 2/2-300 mesh grid, 10nm BSA-gold, 100% humidity, -1.5 to -5 micron defocus</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-9/rawdata/</p>25_dose-filt.st,  Tilt Series (Pixel Size 0.1778 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-9/rawdata/25_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        25_dose-filt.rec,  Reconstruction (Pixel Size 0.7112 nm), 107.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-9/3dimage_102906/25_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_25.mrc.mdoc,  24.7 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-9/rawdata/TS_25.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-9/file_20304/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 7.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-9/file_20305/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kf5n6-3zf74
title: Shewanella OMV delMTR

description: Shewanella outer membrane vesicles, delMTR (cytochromes) knockout, high resolution
description: <strong>Tilt Series Date:</strong> 2018-09-12</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 82000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Lori Zacharoff in the El Naggar lab at USC; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Sample Preparation:</strong> C-Flat 2/2-300 mesh grid, 10nm BSA-gold, 100% humidity, -1.5 to -5 micron defocus</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-18/rawdata/</p>35_dose-filt.st,  Tilt Series (Pixel Size 0.1778 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-18/rawdata/35_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        35_dose-filt.rec,  Reconstruction (Pixel Size 0.7112 nm), 950.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-18/3dimage_102999/35_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_35.mrc.mdoc,  24.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-18/rawdata/TS_35.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 2.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-18/file_20331/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 6.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-18/file_20332/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c6mzp-54s60
title: Mega gas vesicles

description: Mega gas vesicles, purified from E. coli, -1 to -5 micron defocus. Reconstruct with included scripts for ctf-corrected bin1 tomogram.
description: <strong>Tilt Series Date:</strong> 2019-09-25</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Bacillus megaterium</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-48.0°, 51.0°), step: 3°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD 4.9.8, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Rob Hurt and George Lu from the Shapiro lab at Caltech; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas with assistance from Przemek Dutka</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified Mega gas vesicles in 10 mM HEPES</p>
<strong>Sample Preparation:</strong> protochips C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-36/rawdata/</p>36_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-36/rawdata/36_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        36_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 460.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-36/3dimage_121873/36_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_36.mrc.mdoc,  28.1 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-36/rawdata/TS_36.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-36/file_27786/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 25.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-36/file_27787/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/82maj-jtq57
title: H. Neapolitanus Carboxysomes

description: Carboxysomes purified from H. Neapolitanus, WT, high resolution
description: <strong>Tilt Series Date:</strong> 2019-08-21</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Halothiobacillus neapolitanus</p>
<strong>Strain:</strong> c2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, novaCTF, custom bash and Matlab scripts</p>
<strong>Collaborators and Roles:</strong> purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by the Savage lab</p>
<strong>Sample Preparation:</strong> protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-31/rawdata/</p>31_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 4.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-31/rawdata/31_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        31_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 442.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-31/3dimage_113073/31_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 15.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-31/file_26651/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-31/file_26650/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        TS_31.mrc.mdoc,  30.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-31/rawdata/TS_31.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/t1fgj-7gc89
title: Mega gas vesicles

description: Mega gas vesicles, purified from E. coli, -1 to -5 micron defocus. Reconstruct with included scripts for ctf-corrected bin1 tomogram.
description: <strong>Tilt Series Date:</strong> 2019-09-25</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Bacillus megaterium</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-48.0°, 51.0°), step: 3°, constant angular increment, dosage: 70.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD 4.9.8, ctffind4, custom scripts</p>
<strong>Collaborators and Roles:</strong> purified by Rob Hurt and George Lu from the Shapiro lab at Caltech; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas with assistance from Przemek Dutka</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified Mega gas vesicles in 10 mM HEPES</p>
<strong>Sample Preparation:</strong> protochips C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-34/rawdata/</p>34_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-34/rawdata/34_dose-filt.st"
        > <i class="download icon"></i> Download </a></p>

        34_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 460.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-34/3dimage_121869/34_dose-filt.rec"
        > <i class="download icon"></i> Download </a></p>

        TS_34.mrc.mdoc,  28.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-34/rawdata/TS_34.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>

        frames.tar.bz2,  zipped raw frames, 1.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-34/file_27780/frames.tar.bz2"
        > <i class="download icon"></i> Download </a></p>

        scripts.tar.bz2,  zipped processing scripts, 27.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-34/file_27781/scripts.tar.bz2"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ak6zk-dtj21
title: H. Neapolitanus Carboxysomes

description: Carboxysomes purified from H. Neapolitanus, WT, high resolution
description: <strong>Tilt Series Date:</strong> 2019-08-21</p>
<strong>Data Taken By:</strong> LaurenAnn Metskas</p>
<strong>Species / Specimen:</strong> Halothiobacillus neapolitanus</p>
<strong>Strain:</strong> c2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: 0.0 μm,  magnification: 81000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> IMOD, ctffind4, novaCTF, custom bash and Matlab scripts</p>
<strong>Collaborators and Roles:</strong> purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by the Savage lab</p>
<strong>Sample Preparation:</strong> protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-33/rawdata/</p>33_dose-filt.st,  Tilt Series (Pixel Size 0.1104 nm), 4.2 GB  
        
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        33_dose-filt.rec,  Reconstruction (Pixel Size 0.8832 nm), 405.5 MB  
        
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        TS_33.mrc.mdoc,  30.1 kB  
        
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        frames.tar.bz2,  zipped raw frames, 1.9 GB  
        
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        scripts.tar.bz2,  zipped processing scripts, 12.6 kB  
        
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