DOI: 10.5061/dryad.st6h9
title: Data from: Mutational analysis of Rab3 function for controlling active zone protein composition at the Drosophila neuromuscular junction

description: At synapses, the release of neurotransmitter is regulated by molecular
 machinery that aggregates at specialized presynaptic release sites termed
 active zones. The complement of active zone proteins at each site is a
 determinant of release efficacy and can be remodeled to alter synapse
 function. The small GTPase Rab3 was previously identified as playing a
 novel role that controls the distribution of active zone proteins to
 individual release sites at the Drosophila neuromuscular junction. Rab3
 has been extensively studied for its role in the synaptic vesicle cycle;
 however, the mechanism by which Rab3 controls active zone development
 remains unknown. To explore this mechanism, we conducted a mutational
 analysis to determine the molecular and structural requirements of Rab3
 function at Drosophila synapses. We find that GTP-binding is required for
 Rab3 to traffick to synapses and distribute active zone components across
 release sites. Conversely, the hydrolytic activity of Rab3 is unnecessary
 for this function. Through a structure-function analysis we identify
 specific residues within the effector-binding switch regions that are
 required for Rab3 function and determine that membrane attachment is
 essential. Our findings suggest that Rab3 controls the distribution of
 active zone components via a vesicle docking mechanism that is consistent
 with standard Rab protein function.
description: CDR Region Mutation Brp
 Distribution RescueZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderCDR Region Mutation Rab3
 IntensityZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderdeltaC Brp Distribution
 RescueZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderdeltaC Rab3
 IntensityZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderN134I and T35N Brp
 Distribution RescueZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderN134I and T35N Rab3
 IntensityZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderN134I and T35N DN
 PhenotypeZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderQ80L Brp Distribution
 RescueZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderQ80L Rab3
 IntensityZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderQ80L Overexpression in
 WTZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderRab3-GAP Brp
 AreaZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderSwitch Region Mutation
 Brp Distribution RescueZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderSwitch Region Mutation
 Rab3 IntensityZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderT53A Y83A and deltaC in
 WTZipped folder contains
 Multichannel TIFFs. Explanation of files included as .txt file in Zipped
 FolderTraces for mEJC
 QuantificationZipped folder contains
 .ABF Files. Explanation of files included as .txt file in Zipped
 FolderTraces for EJC
 QuantificationZipped folder contains
 .ABF Files. Explanation of files included as .txt file in Zipped
 FolderTraces for Facilitation
 Index QuantificationZipped folder contains
 .ABF Files. Explanation of files included as .txt file in Zipped
 Folder