cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7451975
title: Soil mesofauna in consolidated land use systems: how management affects soil and litter invertebrates

description: ABSTRACT: Soil mesofauna consists of small invertebrates that live in the soil or litter and are sensitive to climatic conditions, management systems, plant cover and physical or chemical soil attributes. These organisms are active in the cycling of nutrients, since they fragment the organic matter hereby accelerating microbial decomposition. The aim of this study was to evaluate the invertebrate community in no-tillage, conventional tillage, minimum tillage and secondary forest in regeneration to determine the relationship of mesofauna to litter, soil attributes, management and seasonality. Therefore, ten soil samples in each system and eight litter samples in no-tillage and the forest were taken over four seasons. These samples remained in Berlese extractors for seven days for quantification and identification of mesofauna. For each fauna sample, soil samples were collected for chemical analysis. Next, diversity indices and richness were calculated and multivariate analyses were used to establish relationships between the mesofauna, soil attributes and management. In the soil, mites were more abundant in the agricultural systems than in the forest, but the springtails, sensitive to low moisture and high temperature, were more abundant in the forest. Diversity and richness were higher in soil from the forest than under other systems. In no-tillage, there was a lower density of soil mesofauna, however, under this system, many invertebrates live in litter, since litter is the main food resource for them. In forest litter, we found lower invertebrate density and higher diversity than in no-tillage. Carbon, basic cations, pH, Al and V% were the attributes that best explained fauna variability in the systems.
DOI: 10.6084/m9.figshare.7451981
title: Primary Health Care federal funding in the Unified Health System: old and new dilemmas

description: ABSTRACT This article aims to discuss the financing of Primary Care in the SUS, seeking to highlight the historical persistence of the fragility of this funding in terms of the transfer model and a limited view of the concept of Primary Care. The article is structured in three parts. The first part discusses the historical trajectory of the concept of Primary Care, from its initial radicalism in the 1960s to the 'downsizing' brought about by the new National Policy of Primary Health Care (PNAB) of 2017. The second part addresses, in a historical perspective, the federal funding of the SUS, with emphasis on Primary Care and the assessment criteria used. The third part deals with the financing of Primary Care, highlighting the resources of the Ministry of Health for this level of care, focusing on the new PNAB 2017 and, in particular, those transferred through the Basic Attention Floor (PAB) Variable.
DOI: 10.6084/m9.figshare.7451981.v1
title: Primary Health Care federal funding in the Unified Health System: old and new dilemmas

description: ABSTRACT This article aims to discuss the financing of Primary Care in the SUS, seeking to highlight the historical persistence of the fragility of this funding in terms of the transfer model and a limited view of the concept of Primary Care. The article is structured in three parts. The first part discusses the historical trajectory of the concept of Primary Care, from its initial radicalism in the 1960s to the 'downsizing' brought about by the new National Policy of Primary Health Care (PNAB) of 2017. The second part addresses, in a historical perspective, the federal funding of the SUS, with emphasis on Primary Care and the assessment criteria used. The third part deals with the financing of Primary Care, highlighting the resources of the Ministry of Health for this level of care, focusing on the new PNAB 2017 and, in particular, those transferred through the Basic Attention Floor (PAB) Variable.
DOI: 10.6084/m9.figshare.7451972
title: Characterisation of plasmid-mediated rmtB-1 in Enterobacteriaceae clinical isolates from São Paulo, Brazil

description: OBJECTIVES The emergence of 16S rRNA methyltranferases (16 RMTAses) has jeopardised the clinical use of aminoglycosides. RmtB is one of the most frequently reported in Gram-negatives worldwide. In this study, we aimed to estimate the frequency of 16S RMTAses encoding genes in Enterobacteriaceae isolated in a three-month period from a tertiary Brazilian hospital. METHODS All Gram-negatives classified as resistant to amikacin, gentamicin, and tobramycin by agar screening were selected for analysis. The presence of 16SRMTases encoding genes was verified by polymerase chain reaction (PCR). Antimicrobial susceptible profile was determined by broth microdilution. The genetic relationship among these isolates was accessed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Selected RmtB-producing isolates were characterised by whole genome sequencing (WGS) analysis. RESULTS Twenty-two of 1,052 (2.1%) Enterobacteriaceae were detected as producers of RmtB-1 [Klebsiella pneumoniae (n = 21) and Proteus mirabilis (n = 1)]. blaKPC-2 was identified among 20 RmtB-1-producing K. pneumoniae isolates that exhibited an identical PFGE and MLST (ST258) patterns. Two K. pneumoniae isolates, the A64216 (not harboring bla KPC-2), A64477 (harboring bla KPC-2) and one P. mirabilis isolate (A64421) were selected for WGS. rmtB-1 and bla KPC-2 genes were carried by distinct plasmids. While a plasmid belonging to the IncFIIk group harbored rmtB-1 in K. pneumoniae, this gene was carried by a non-typable plasmid in P. mirabilis. In the three analysed plasmids, rmtB-1 was inserted on a transposon, downstream a Tn2. CONCLUSION Our findings suggested that the rmtB-1 was harbored by plasmids distinct from those previously reported in Bolivia and China. It suggests that multiple mobilization events might have occurred in South America.
DOI: 10.6084/m9.figshare.7451975.v1
title: Soil mesofauna in consolidated land use systems: how management affects soil and litter invertebrates

description: ABSTRACT: Soil mesofauna consists of small invertebrates that live in the soil or litter and are sensitive to climatic conditions, management systems, plant cover and physical or chemical soil attributes. These organisms are active in the cycling of nutrients, since they fragment the organic matter hereby accelerating microbial decomposition. The aim of this study was to evaluate the invertebrate community in no-tillage, conventional tillage, minimum tillage and secondary forest in regeneration to determine the relationship of mesofauna to litter, soil attributes, management and seasonality. Therefore, ten soil samples in each system and eight litter samples in no-tillage and the forest were taken over four seasons. These samples remained in Berlese extractors for seven days for quantification and identification of mesofauna. For each fauna sample, soil samples were collected for chemical analysis. Next, diversity indices and richness were calculated and multivariate analyses were used to establish relationships between the mesofauna, soil attributes and management. In the soil, mites were more abundant in the agricultural systems than in the forest, but the springtails, sensitive to low moisture and high temperature, were more abundant in the forest. Diversity and richness were higher in soil from the forest than under other systems. In no-tillage, there was a lower density of soil mesofauna, however, under this system, many invertebrates live in litter, since litter is the main food resource for them. In forest litter, we found lower invertebrate density and higher diversity than in no-tillage. Carbon, basic cations, pH, Al and V% were the attributes that best explained fauna variability in the systems.
DOI: 10.7284/101855
title: Gravimeter data collected during research cruise MV1012 using a Bell BGM-3 instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7451969
title: Polymorphisms in the TGFB1 and IL2RA genes are associated with clinical forms of leprosy in Brazilian population

description: BACKGROUND Leprosy is a chronic infectious disease caused by Mycobacterium leprae, and compromises the skin and peripheral nerves. This disease has been classified as multibacillary (MB) or paucibacillary (PB) depending on the host immune response. Genetic epidemiology studies in leprosy have shown the influence of human genetic components on the disease outcomes. OBJECTIVES We conducted an association study for IL2RA and TGFB1 genes with clinical forms of leprosy based on two case-control samples. These genes encode important molecules for the immunosuppressive activity of Treg cells and present differential expressions according to the clinical forms of leprosy. Furthermore, IL2RA is a positional candidate gene because it is located near the 10p13 chromosome region, presenting a linkage peak for PB leprosy. METHODS A total of 885 leprosy cases were included in the study; 406 cases from Rondonópolis County (start population), a hyperendemic region for leprosy in Brazil, and 479 cases from São Paulo state (replication population), which has lower epidemiological indexes for the disease. We tested 11 polymorphisms in the IL2RA gene and the missense variant rs1800470 in the TGFB1 gene. FINDINGS The AA genotype of rs2386841 in IL2RA was associated with the PB form in the start population. The AA genotype of rs1800470 in TGFB1 was associated with the MB form in the start population, and this association was confirmed for the replication population. MAIN CONCLUSIONS We demonstrated, for the first time, an association data with the PB form for a gene located on chromosome 10. In addition, we reported the association of TGFB1 gene with the MB form. Our results place these genes as candidates for validation and replication studies in leprosy polarisation.
DOI: 10.6084/m9.figshare.7451966
title: Foliar anatomy of Rhynchosia spp. (Leguminosae, Papilionoideae) from Roraima state, northern Brazilian Amazon

description: ABSTRACT Comparative studies on the structure of foliar anatomy in four species of Rhynchosia from Roraima state (northern Brazilian Amazon) were carried out to identify additional morphological characters to support the definition of the systematic boundaries among the species. Fully expanded leaves, including the petioles, were collected from the upper nodes of a plant’s stem. Anatomical characters were observed in cross-sections of the middle portion of leaflets and petiole segments. Presence and distribution of glandular trichomes, continuity of sclerenchyma around the vascular bundles in leaflet blades and the conformation of the area between the two ridges of petioles were important characters to distinguish among the species of Rhynchosia. For the first time we report a distinct multicellular gland-like structure which we found in the petioles of three species.
DOI: 10.6084/m9.figshare.7451969.v1
title: Polymorphisms in the TGFB1 and IL2RA genes are associated with clinical forms of leprosy in Brazilian population

description: BACKGROUND Leprosy is a chronic infectious disease caused by Mycobacterium leprae, and compromises the skin and peripheral nerves. This disease has been classified as multibacillary (MB) or paucibacillary (PB) depending on the host immune response. Genetic epidemiology studies in leprosy have shown the influence of human genetic components on the disease outcomes. OBJECTIVES We conducted an association study for IL2RA and TGFB1 genes with clinical forms of leprosy based on two case-control samples. These genes encode important molecules for the immunosuppressive activity of Treg cells and present differential expressions according to the clinical forms of leprosy. Furthermore, IL2RA is a positional candidate gene because it is located near the 10p13 chromosome region, presenting a linkage peak for PB leprosy. METHODS A total of 885 leprosy cases were included in the study; 406 cases from Rondonópolis County (start population), a hyperendemic region for leprosy in Brazil, and 479 cases from São Paulo state (replication population), which has lower epidemiological indexes for the disease. We tested 11 polymorphisms in the IL2RA gene and the missense variant rs1800470 in the TGFB1 gene. FINDINGS The AA genotype of rs2386841 in IL2RA was associated with the PB form in the start population. The AA genotype of rs1800470 in TGFB1 was associated with the MB form in the start population, and this association was confirmed for the replication population. MAIN CONCLUSIONS We demonstrated, for the first time, an association data with the PB form for a gene located on chromosome 10. In addition, we reported the association of TGFB1 gene with the MB form. Our results place these genes as candidates for validation and replication studies in leprosy polarisation.
DOI: 10.6084/m9.figshare.7451972.v1
title: Characterisation of plasmid-mediated rmtB-1 in Enterobacteriaceae clinical isolates from São Paulo, Brazil

description: OBJECTIVES The emergence of 16S rRNA methyltranferases (16 RMTAses) has jeopardised the clinical use of aminoglycosides. RmtB is one of the most frequently reported in Gram-negatives worldwide. In this study, we aimed to estimate the frequency of 16S RMTAses encoding genes in Enterobacteriaceae isolated in a three-month period from a tertiary Brazilian hospital. METHODS All Gram-negatives classified as resistant to amikacin, gentamicin, and tobramycin by agar screening were selected for analysis. The presence of 16SRMTases encoding genes was verified by polymerase chain reaction (PCR). Antimicrobial susceptible profile was determined by broth microdilution. The genetic relationship among these isolates was accessed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Selected RmtB-producing isolates were characterised by whole genome sequencing (WGS) analysis. RESULTS Twenty-two of 1,052 (2.1%) Enterobacteriaceae were detected as producers of RmtB-1 [Klebsiella pneumoniae (n = 21) and Proteus mirabilis (n = 1)]. blaKPC-2 was identified among 20 RmtB-1-producing K. pneumoniae isolates that exhibited an identical PFGE and MLST (ST258) patterns. Two K. pneumoniae isolates, the A64216 (not harboring bla KPC-2), A64477 (harboring bla KPC-2) and one P. mirabilis isolate (A64421) were selected for WGS. rmtB-1 and bla KPC-2 genes were carried by distinct plasmids. While a plasmid belonging to the IncFIIk group harbored rmtB-1 in K. pneumoniae, this gene was carried by a non-typable plasmid in P. mirabilis. In the three analysed plasmids, rmtB-1 was inserted on a transposon, downstream a Tn2. CONCLUSION Our findings suggested that the rmtB-1 was harbored by plasmids distinct from those previously reported in Bolivia and China. It suggests that multiple mobilization events might have occurred in South America.
DOI: 10.6084/m9.figshare.7451963
title: Yellow fever: laboratorial diagnosis and clinical manifestations

description: ABSTRACT Yellow fever is an infectious disease of acute evolution, initially non-contagious, transmitted by a ribonucleic acid (RNA) virus that belongs to the Flaviviridae family. In the period from December 2016 until March 17, 2017, 1, 561 suspected cases of wild yellow fever were reported to the Ministry of Health in Brazil. Among these cases, 850 (54.8%) remain under investigation, 448 (28.7%) were confirmed and 263 (16.9%) were discarded. Out of the total cases reported, 264 died, 144 (54.5%) were confirmed for the disease, 110 (41.7%) were investigated and 10 (3.8%) were discarded. The case fatality rate among confirmed cases was 32.2%. The specific diagnosis for determining the etiology of infection can be made by demonstrating the humoral response of the antibodies, virus isolation, or histopathological study of the liver. Only through early laboratory diagnosis and epidemiological data supply can government and cooperative organizations establish public policies to combat future disease epidemics, as well as social awareness campaigns.
DOI: 10.7284/101856
title: Meteorological Station data collected during research cruise MV1012 using a SIO MET-System instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7451966.v1
title: Foliar anatomy of Rhynchosia spp. (Leguminosae, Papilionoideae) from Roraima state, northern Brazilian Amazon

description: ABSTRACT Comparative studies on the structure of foliar anatomy in four species of Rhynchosia from Roraima state (northern Brazilian Amazon) were carried out to identify additional morphological characters to support the definition of the systematic boundaries among the species. Fully expanded leaves, including the petioles, were collected from the upper nodes of a plant’s stem. Anatomical characters were observed in cross-sections of the middle portion of leaflets and petiole segments. Presence and distribution of glandular trichomes, continuity of sclerenchyma around the vascular bundles in leaflet blades and the conformation of the area between the two ridges of petioles were important characters to distinguish among the species of Rhynchosia. For the first time we report a distinct multicellular gland-like structure which we found in the petioles of three species.
DOI: 10.6084/m9.figshare.7451963.v1
title: Yellow fever: laboratorial diagnosis and clinical manifestations

description: ABSTRACT Yellow fever is an infectious disease of acute evolution, initially non-contagious, transmitted by a ribonucleic acid (RNA) virus that belongs to the Flaviviridae family. In the period from December 2016 until March 17, 2017, 1, 561 suspected cases of wild yellow fever were reported to the Ministry of Health in Brazil. Among these cases, 850 (54.8%) remain under investigation, 448 (28.7%) were confirmed and 263 (16.9%) were discarded. Out of the total cases reported, 264 died, 144 (54.5%) were confirmed for the disease, 110 (41.7%) were investigated and 10 (3.8%) were discarded. The case fatality rate among confirmed cases was 32.2%. The specific diagnosis for determining the etiology of infection can be made by demonstrating the humoral response of the antibodies, virus isolation, or histopathological study of the liver. Only through early laboratory diagnosis and epidemiological data supply can government and cooperative organizations establish public policies to combat future disease epidemics, as well as social awareness campaigns.
DOI: 10.7284/101859
title: Multibeam Echosounder data collected during research cruise MV1012 using a Kongsberg EM122 instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7451948
title: Influence of socio-demographic characteristics in the self-care of people with heart failure

description: ABSTRACT Objective: To determine the influence of socio-demographic characteristics in the self-care of people with heart failure (HF). Method: Cross-sectional, analytical study, held in three private hospitals in Fortaleza, Ceará, Brasil, with 57 hospitalized patients. The data were collected through a demographic characterization form and a self-care assessment scale and were analyzed with inferential statistics, using mean comparison tests. Results: Self-care was best assessed in people with higher education level, higher household income and in a relationship. Conclusion: The socio-demographic characteristics influenced seven self-care practices: dietary control; monitoring of body weight; effort in labor activities; knowledge about HF; up-to-date vaccination record; leisure activities; and family and social support network with strong bonds. The higher prevalence of answers indicating satisfactory self-care practices among the patients occurred in the areas of health promotion and tolerance to stress.
DOI: 10.6084/m9.figshare.7451951
title: Chemical characterization of winemaking byproducts from grape varieties cultivated in Vale do São Francisco, Brazil

description: Abstract The recovery of agroindustry byproducts can bring opportunities for the development of new products. The objective of this study was to characterize chemical compounds contents in the byproducts generated during winemaking from different grape varieties cultivated in Vale do São Francisco, Brazil. The grapes and their byproducts (skins and seeds) from four wineries (w1, w2, w3 and w4) were evaluated. The varieties studied were: Viognier, Chenin Blanc, Moscato Canelli, Italia, Arinto+Fernão Pires, Tempranillo, Grenache, Mourvèdre, Verdejo and Sauvignon Blanc, used in the processing of white/sparkling wines, and also Tempranillo, Cabernet Sauvignon, Syrah and Alicante Bouschet, used in the processing of red wines. High ascorbic acid contents were found in the skin and seed byproducts, the latter also presenting high protein and pectin contents. The skin byproduct obtained in the process of elaborating white/sparkling wines from ‘Tempranillo’ grapes presented relatively higher ascorbic acid, pectin and protein contents. In the elaboration of red wines by w1, the ‘Syrah’ skin byproduct showed high ascorbic acid, soluble solids, sugars and protein contents. The seed byproduct from the ‘Syrah’ grapes used to elaborate red wines by w1 showed a more favorable composition for reuse, as did those of red wines of ‘Tempranillo’ grapes from w3.
DOI: 10.6084/m9.figshare.7451951.v1
title: Chemical characterization of winemaking byproducts from grape varieties cultivated in Vale do São Francisco, Brazil

description: Abstract The recovery of agroindustry byproducts can bring opportunities for the development of new products. The objective of this study was to characterize chemical compounds contents in the byproducts generated during winemaking from different grape varieties cultivated in Vale do São Francisco, Brazil. The grapes and their byproducts (skins and seeds) from four wineries (w1, w2, w3 and w4) were evaluated. The varieties studied were: Viognier, Chenin Blanc, Moscato Canelli, Italia, Arinto+Fernão Pires, Tempranillo, Grenache, Mourvèdre, Verdejo and Sauvignon Blanc, used in the processing of white/sparkling wines, and also Tempranillo, Cabernet Sauvignon, Syrah and Alicante Bouschet, used in the processing of red wines. High ascorbic acid contents were found in the skin and seed byproducts, the latter also presenting high protein and pectin contents. The skin byproduct obtained in the process of elaborating white/sparkling wines from ‘Tempranillo’ grapes presented relatively higher ascorbic acid, pectin and protein contents. In the elaboration of red wines by w1, the ‘Syrah’ skin byproduct showed high ascorbic acid, soluble solids, sugars and protein contents. The seed byproduct from the ‘Syrah’ grapes used to elaborate red wines by w1 showed a more favorable composition for reuse, as did those of red wines of ‘Tempranillo’ grapes from w3.
DOI: 10.7284/101857
title: Singlebeam Echosounder data collected during research cruise MV1012 using a Knudsen 320B/R instrument system onboard the platform RV Melville

description: Original (unprocessed) data collected during a Field_expedition