DOI: 10.5061/dryad.z612jm6j9
title: Morphometrics in the recurved corolla clade of Burmeistera (Campanulaceae)

description: In this study, we used morphometric data to test species boundaries in the
 genus Burmeistera (Campanulaceae). Morphometrics measurements were made on
 herbarium specimens from a monophyletic clade of three species with
 recurved corolla lobes: B. crispiloba, B. sodiroana, B.
 succulenta. We used using both hierarchical and normal mixture model-based
 clustering methods to test the current species hypotheses. Our results
 support the recognition of the three known species plus a new species
 described in the paper.
description: Morphometric measurements were made from 95 herbarium specimens
 using a ruler and digital calipers. The herbarium specimens were sourced
 from three herbaria: MO, NY, and QCA. Measurements include 23 continuous
 quantitative variables and two qualitative variables, which are described
 in more detail in the README file. The measurements are
 unprocessed.
description: # Morphometrics in the Recurved Corolla Clade of Burmeistera
 (Campanulaceae) This REAME
 file was generated on 2023-11-29 by Brock Mashburn. GENERAL INFORMATION 1.
 Title: Morphometrics in the Recurved Corolla Clade of Burmeistera
 (Campanulaceae) 2. Author Information 1. Brock Mashburn; Department of
 Biology, University of Missouri–St. Louis, St. Louis, Missouri 63121,
 U.S.A. [brockmashburn@gmail.com](mailto:brockmashburn@gmail.c) 2. Andrea
 Trigueros; Department of Biology, University of Missouri–St. Louis, St.
 Louis, Missouri 63121, U.S.A. 3. Carmen Ulloa Ulloa; Missouri Botanical
 Garden, 4344 Shaw, St. Louis, Missouri 63110, U.S.A. 4. Nathan Muchhala;
 Department of Biology, University of Missouri–St. Louis, St. Louis,
 Missouri 63121, U.S.A. 3. Date of data collection: 2017-2018 4. Funding
 sources: National Science Foundation under Grant No. 1754802 awarded to
 Nathan Muchhala DATA & FILE OVERVIEW 1. File List: 1.
 Morphometric_measurements_RCC.csv 2. RCC_code.R
 \#########################################################################
 DATA-SPECIFIC INFORMATION FOR: Morphometric_measurements_RCC.csv 1. Number
 of variables: 33 2. Number of samples (rows): 95 3. Variable list 1.
 sample_num: samples were numbered 1-95 to assist in tracking samples in R
 2. pile_id1: a priori assignment of samples to species (*B. crispiloba, B.
 sodiroana, B. succulenta*) 3. pile_id2: a priori assignment of samples to
 species or assignment to 'intermediate' between *B. crispiloba*
 and *B. sodiroana*. 4. collector: surname of collector of the herbarium
 specimen 5. coll_num: collection number of the herbarium specimen 6.
 country: country of collection 7. latitude: decimal latitude of
 collection, either from specimen label or georeferenced by us 8.
 longitude: decimal longitude of collection, either from specimen label or
 georeferenced by us 9. phyllotaxy: a qualitative character, one of two
 options: 'distichous' or 'spiral' 10. petiole_maxlen:
 length of longest petiole on the specimen, millimeters 11. petiole_minlen:
 length of shortest petiole, millimeters 12. small_leaf_len: length of
 smallest leaf, millimeters 13. small_leaf_wid: width of smallest leaf,
 millimeters 14. large_leaf_len: length of largest leaf, millimeters 15.
 large_leaf_wid: width of largest leaf, millimeters 16. apex_len: length of
 apex from largest leaf, millimeters 17. apex_wid: width of base of apex
 from largest leaf, millimeters 18. fert_leaf_len: length of first leaf
 subtending a flower, millimeters 19. fert_leaf_wid: width of first leaf
 subtending a flower, millimeters 20. pedicel_len: length of longest
 pedicel on specimen, millimeters 21. hyp_len: length of hypanthium,
 millimeters 22. hyp_wid: width at widest point of hypanthium, millimeters
 23. calyxlobe_len: length of longest calyx lobe, millimeters 24.
 calyxlobe_wid: width of the same calyx lobe as above, millimeters 25.
 andr_len: length of androecium, millimeters 26. cortube_dors_len: length
 of corolla tube from base to dorsal opening, millimeters 27.
 cortube_vent_len: length of corolla tube from base to ventral opening,
 millimeters 28. cortube_narrow_wid: width of the narrowest point of
 corolla tube, millimeters 29. corolla_curling: a qualitative character
 describing the curling of the corolla lobes, one of three options:
 'none', 'curl', or 'scroll' 30.
 anth_tub_len: length of anther tube, millimeters 31. anth_tip_wid: width
 at tip of anther tube, millimeters 32. exsertion_len: length of androecium
 from corolla tube opening to anther tube tip, millimeters 33. flower_len:
 length of whole flower from base of hypanthium to anther tube tip,
 millimeters 4. Missing data codes: NA 5. Specialized formats or other
 abbreviations used: None The main dataset is Appendix 1, an excel file
 with morphometric measurements of herbarium specimens. An additional .R
 file includes all data analysis and figure production. All other files are
 supplemental figures and appendices resulting from analyses in the .R
 file.
 \#########################################################################
 SPECIFIC INFORMATION FOR: RCC_code.R The program R is required to run this
 file. Required packages are listed within the file. SHARING/ACCESS
 INFORMATION 1\. Licenses/restrictions placed on the data: CC0 1.0
 Universal (CC0 1.0) Public Domain 2\. Links to publications that cite or
 use the data: Mashburn, B, Trigueros, A, Ulloa Ulloa, C, Muchhala, N.
 (2023/2024). Morphometrics in the Recurved Corolla Clade of Burmeistera
 (Campanulaceae) Clarifies Species Limits and Identifies a New Species.
 Systematic Botany.