cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14288628
title: EVALUATION OF THE INDICATORS OF INFLAMMATION IN THE DIAGNOSIS OF BOVINE MASTITIS

description: ABSTRACT The aim of this trial was to evaluate and compare the distinct indictors of inflammation in the diagnosis of mastitis. The somatic cell count (SCC), the California Mastitis Test score, the electrical conductivity, the hydrogen ion concentration (pH), the chloride content and the bacteriological status of 321 milk samples from mammary glands without any clinical signs were analyzed. Their predictor value as an indicator of inflammation was calculated considering the bacteriologic examination as the gold standard test. Then, the sensitivity, specificity, positive predictive value, negative predictive value and likelihood ratio at various cut-off values were established. The bacteriologic exam resulted in 46.73% of the samples being positive, with Staphylococcus sp. the main genus isolated. The SCC and CMT proved to be the best predictors. On the other hand, pH, electrical conductivity and chloride content did not prove to be reliable diagnostic indicators when used in isolation.
DOI: 10.6084/m9.figshare.14288626
title: EFFECTIVENESS OF TRIAZOLE FUNGICIDES FOR THE CONTROL OF BROWN EYE SPOT ON COFFEE SEEDLINGS

description: ABSTRACT Triazole fungicides and their mixtures with insecticides are usually employed for the control of coffee rust and leaf miner in coffee crops. In this study these chemicals were evaluated for the control of brown eye spot, caused by Cercospora coffeicola, another important disease of this crop. Three experiments were carried out in a greenhouse with coffee seedlings of the cultivar Mundo Novo IAC 501. The fungicides epoxiconazole, epoxiconazole + pyraclostrobin, tetraconazole, ciproconazole, copper oxychloride, azoxystrobin and carbendazim were applied to the leaves of coffee seedlings, and ciproconazole + tiametoxam, triadimenol + dissulfotom, and triadimenol were applied to the seedlings substrate. Controls were also included in all experiments. The seedlings were inoculated with suspensions prepared with a mixture of five C. coffeicola isolates (3-4 conidia/mL). The incidence and severity of the disease were evaluated 20 to 56 days after the inoculations. With the exception of copper oxychloride, all products were efficient for the control of brown eye spot. The triazoles, with the exception of tetraconazole, showed reversible phytotoxic effects. Ciproconazole + tiametoxam, epoxiconazole + pyraclostrobin and carbendazim had the highest residual effects and maintained small levels of the disease until 56 days after their application. The triazoles used for the control of coffee rust are also effective for the control of brown eye spot.
DOI: 10.6084/m9.figshare.14288622
title: SCREENING OF BACILLUS THURINGIENSIS BERLINER AGAINST TETRANYCHUS URTICAE KOCH.

description: ABSTRACT The two-spotted spider mite is the most important pest to Brazilian crops. The incorrect use of chemical control leads to serious consequences, such as the presence of residues in fruits and the emergence of resistant populations. There is a need for alternative methods for managing the mite, such as biological control, which has been used as a tool in the management of various pests of agricultural importance in the world. Bioassays were carried out to analyze 120 strains of Bacillus thuringiensis Berliner in regard to their pathogenicity to Tetranychus urticae Koch. Suspensions were prepared of the different strains, containing 3 × 108 spores/mL of distilled water, and applied on Jack-bean (Canavalia ensiformes DC) leaf disks. Each strain constituted a treatment, with 8 repetitions, each containing 10 adult Tetranychus urticae females. The tests in laboratory conditions showed that mortality was significantly, ranging from 6.7% (strains LMJ7A) to 54.9% (strains 1077B and 886f).
DOI: 10.6084/m9.figshare.14288621
title: EFFECT OF INSECTICIDAL PLANTS ON THE BIOLOGY AND BEHAVIOR OF PLUTELLA XYLOSTELLA (LEPIDOPTERA: PLUTELLIDAE).

description: ABSTRACT The effect of aqueous extracts of Azadirachta indica A. Juss., Sapindus saponaria L., Nim + Piretro + Rotenona, Dimorphandra mollis and Stryph nodendron adstringens (Mart) Coville was evaluated in relation to non-preference for oviposition and feeding, attractiveness and biology of Plutella xylostella. For the experiment, leaf discs from cabbage (Brassica oleracea var. acephala) plants of the Butter cultivar were immersed in each extract at the concentration 10% (weight/volume) for 30 seconds. For the no-choice test the treatments that presented less attractiveness were the extracts of A. indica and D. mollis, and the consumption was lower in A. indica. The extracts of A. indica. S. saponaria. D. mollis and S. adstringens provided a deterrent effect on oviposition of P. xylostella. The treatments A. indica. S. saponaria and S. adstringens showed a negative effect for the biological parameters of the pest.
DOI: 10.6084/m9.figshare.14288619
title: PESTICIDE EFFECTS ON THE PATHOGENICITY OF CONIDIA OF LECANICILLIUM LECANII TO TETRANYCHUS URTICAE

description: ABSTRACT The objective of this study was to evaluate the effect of pesticides on the pathogenicity of conidia of Lecanicillium lecanii, strain JAB 02, produced in culture media containing acaricides and insecticides, to the two-spotted mite Tetranychus urticae. A blend was made of insecticides (acephate, cyromazine, endosulfan, formetanate hydrochloride, imidacloprid, methiocarb, methomyl, monocrotophos, thiamethoxam) and acaricides (abamectin and propargite) in the culture media PDA, that was poured into a Petri dishes. After solidification, the fungus inoculation was realized at three points and the dishes were incubated for 16 days at a temperature of 25.5 ± 0.5º C and 12 hours of photophase. After the fungus growth, the colonies were cut out and removed to test tubes containing distilled water and detergent Tween 80Ò. The fungus suspension obtained for each treatment was standardized at 1 x 107 con./mL and 1 mL was sprayed on Canavalia ensiformis leaves. After the spraying, 15 mites were placed on each leaf disk and after 2, 4 and 6 days the mortality was evaluated in a stereoscopic microscope. The presence of acaricides and insecticides in the culture media did not affect the pathogenicity of conidia of L. lecanii, which on the 6th evaluation day presented a confirmed mortality in the range of 47 to 76% on two-spotted mites T. urticae.
DOI: 10.6084/m9.figshare.14288618
title: EFFICIENCY AND ECONOMIC VIABILITY OF APPLICATION OF FUNGICIDES IN THE CONTROL OF ASIATIC RUST OF SOYBEANS IN CAMPO GRANDE, MS, BRAZIL

description: ABSTRACT The present study evaluated the efficiency and the economic viability of the application of fungicides to control Asian soybean rust, Phakopsora packyrhizi, in Campo Grande, state of Mato Grosso do Sul, Brazil. The assay was carried in the 2007-2008 harvest. The following fungicides were evaluated (g a.i./ha): picoxystrobin + cyproconazol (40 + 16, 50 + 20 and 60 +24) + nimbus 0.25% (v/v); pyraclostrobin + epoxiconazol (66.5 + 25); azoxystrobin + cyproconazol (60 + 24) + nimbus 0.25% (v/v); trifloxystrobin + tebuconazol (50 + 100) + auero 0.13% (v/v) and trifloxystrobin + cyproconazol (56.25 + 24) + aureo 0.13% (v/v). The fungicide treatments started from the phenologic stage R2. Three applications were realized, 15 days apart. The disease severity (upper and lower part of the plant) was quantified weekly, totaling 10 evaluations. In addition, at the R7 stage, the defoliation was evaluated every seven days. The curves of progress (CP) were plotted and calculated the areas under the CP for the disease severity (AACPD) and defoliation (AACPDes). At the end of the assay, the grain yield (Rend - kg/ha), the mass of 1,000 grains (MMG - g) and the economic viability of the disease control were evaluated. The climate during the conduction of the experiment was favorable to the development of Asian soybean rust. There was a positive relationship between rainfall and severity of the disease. All fungicides showed AACPD below the control, especially picoxystrobin + cyproconazol (higher fungicide dose). This treatment also showed the lowest rate of AACPDes. The fungicides presented similar rates of Rend, although higher than the control treatment. In relation to MMG, the highest index was attributed to azoxystrobin + cyproconazol. The mixture trifloxystrobin + tebuconazol showed the lowest efficiency for the control of Asian soybean rust. Based on the severity of the disease, grain yield and the economic benefits generated by the application of fungicides, picoxystrobin + cyproconazol (60 + 24), azoxystrobin + cyproconazol and trifloxystrobin + cyproconazol were identified as the most promising for the control of P. packyrhizi.
DOI: 10.6084/m9.figshare.14288615
title: POTENTIAL USE OF ENTOMOPATHOGENIC FUNGI IN THE CONTROL OF TETRANYCHUS URTICAE KOCH (ACARI: TETRANYCHIDAE) IN PAPAYA: CULTIVAR EFFECT ON PATOGENICITY

description: ABSTRACT Papaya Carica papaya (L.) is affected by various phytosanitary problems, and the mite Tetranychus urticae (Koch, 1836) (Acari: Tetranychidae) is a key pest. The use of entomopathogenic fungi is an alternative to the use of pesticides, lowering the risk of resistance while reducing the the load of toxic substances in the environment. This study was conducted to determine whether the different cultivars of papaya interfere with the pathogenicity of Beauveria bassiana. Metarhizium anisopliae and Lecanicillium longisporum. The bioas say was carried out using adult T. urticae females maintained for at least one generation on leaves of four papaya cultivars (Calimosa, Golden, Sunrise, Tainung 01) or on jack bean leaves (control). Ten females were transferred to Petri dishes with leaf disks of the origin cultivars and jack beans. The mites were then sprayed with a 5 x 107 conidia.mL-1 suspension in a Potter Tower. Conidial suspensions were prepared from the commercial formulations Boveril® B. bassiana, Metarril® M. anisopliae, and Vertirril® L. longisporum. The corrected and confirmed mortalities were evaluated. Differences were observed (Tukey, P &lt; 0.05) among the papaya cultivars in terms of the T. urticae pathogenic response. On the 3rd day after spraying (DAS), a better efficiency in mortality percentage was observed for the Vertirril® formulation applied to the Calimosa, Golden and Sunrise cultivars, and for Metarril® applied to the Tainung 01cultivar. On the 5th DAS, the efficiencies among the cultivars were very close.
DOI: 10.6084/m9.figshare.14288613
title: SURVEY AND POPULATIONAL DYNAMICS OF PARASITOIDS OF FRUIT FLIES (DIPTERA: TEPHRITIDAE) OCCURING IN GUAVA (PSIDIUM GUAJAVA L.) IN FORTALEZA, CEARÁ, BRAZIL

description: ABSTRACT This study was aimed to identify the parasitoids associated to fruit-fly species (Diptera: Tephritidae) occurring in an urban guava (Psidium guajava L.) (Myrtaceae) orchard located in Fortaleza, Ceará State, Brazil, as well as to determine the populational dynamics of these organ isms in the ecosystem. Ripe and newly fallen guava fruits, from November 1999 to December 2000 were collected. The parasitoids obtained belong to the families Braconidae, Chalcididae, Figitidae (Eucoilinae), Diapriidae (Diapriinae) and Eulophidae. The most common parasitoids belong to the family Figitidae. The highest occurrences were in January and March 2000.
DOI: 10.6084/m9.figshare.14288611
title: EPIDEMIOLOGICAL ANALYSIS OF THE BOVINE TUBERCULOSIS CONDEMNATIONS IN ABATTOIRS IN THE STATE OF SÃO PAULO, BRAZIL

description: ABSTRACT Analyzing data and samples collected at abattoirs in the state of São Paulo, Brazil, the present study provided a profile of the tuberculosis condemned bovine and characterized their herds. From May/2002 to January/2004, tuberculosis lesions from 587 animals were sampled in 79 abattoirs, and from these samples a total of 253 Mycobacterium bovis isolates were obtained. The herds of these animals were tracked down and an epidemiological questionnaire was applied. A dataset about the condemned carcasses was attached to each sample. The data analyses showed that bovine tuberculosis in São Paulo state is concentrated in dairy herds with some sophistication of the production system and mostly affects dairy cows of European breeds older than 36 months. Moreover, the owners of infected herds sell animals without testing them for tuberculosis, contributing to the spread of the disease.
DOI: 10.6084/m9.figshare.14288610
title: INFLUENCE OF HYGIENE MANAGEMENT ON GASTROINTESTINAL HELMINTH INFECTION IN PIGS IN METROPOLITAN RECIFE AND THE COASTAL ZONE OF THE STATE OF PERNAMBUCO, BRAZIL

description: ABSTRACT The aim of the present study was to analyze the influence of management systems at intensive high-tech farms and subsistence farms on the prevalence of swine gastrointestinal helminthes in metropolitan Recife and the coastal zone of the state of Pernambuco, Brazil. For such, the slaughtering process at three slaughterhouses registered with the state inspection system was followed up between July 2008 to May 2009, totaling 447 pigs from 11 properties (8 high-tech farms and 3 subsistence farms). Fecal samples were collected and the parasitological examinations were carried out using the modified McMaster technique and coproculture for identification of larvae. A questionnaire was administered to determine the management condition of each farm. Strongyloidea eggs were present in 2.5% (11/447) of the pigs, and 0.2% (1/447) were positive for Trichuris sp. Coproculture revealed that 5.5% (19/349) were positive for helminthes, 2.9% (10/349) for Hyostrongylus sp., 0.3% (1/349) for Trichostrongylus sp., 2.0% (7/349) for Oesophagostomum sp., and 0.3% (1/349) with mixed infection by Hyostrongylus and Oesophagostomum sp. The occurrence of helminthes was significantly associated (p &lt; 0.05) with helminth gender, feeding, veterinary care, quarantine, live animal trade, anti-helminth agents, water supply, water treatment, hygiene quality of stalls, dung removal frequency and knowledge regarding worms and milk spots. The percentages of positivity were significantly higher among animals on the subsistence farms.
DOI: 10.6084/m9.figshare.14288609
title: BIOLOGIC STUDIES OF MEALYBUGS OF THE GENUS PLANOCOCCUS (HEMIPTERA: PSEUDOCOCCIDAE) ON DIFFERENT HOSTS.

description: ABSTRACT This work was carried out to study the development of populations of the genus Planococcusin different plant species, aiming at the verification of host specificity. The mealybugs Planococcus minorwere obtained from cocoa plants (Theobroma cacaoL.) and coffee crop (Coffea canephoraL.), andPlanococcus citrifrom citrus seedlings (Citrus sinensisL. Osbeck). Single eggs were transferred to Petri dishes containing leaf discs of cocoa (T. cacaocultivar Catongo), coffee (Coffea arabicaL. cultivar Mundo Novo) and citrus (C. sinensiscultivar Pêra Rio). They were maintained on a water-agar slide at 1%, with the abaxial surface turned upwards. The dishes were sealed with a PVC plastic film and kept in climatized chambers regulated at 25 ± 1º C, 70 ± 10% RH and 12 hours of photophase. For the mealybugs coming from cocoa plants, the citrus substrate prolonged the nymphal development of the males (26.0 days). In the nymphal period of females and males coming from coffee plants, the coffee substrate allowed a shorter duration of that period (19.2 and 21.3 days, respectively) and, in addition to having providing a longer longevity to the females (59.1 days). The highest rates of mortality were obtained when they were reared on the cocoa substrate, regardless of the host plant from which they were originally collected, and when kept on citrus, for insects collected on coffee plants. P. citriand P. minorhave higher preference for coffee substrate regardless of the original host, thus showing partial host specificity.
DOI: 10.6084/m9.figshare.14288606
title: COMPARISON OF AGGLUTINATING AND GROWTH INHIBITION ANTIBODIES LEVELS IN SOWS IMMUNIZED WITH TWO EXPERIMENTAL INACTIVATED MONOVALENT VACCINES OF CANICOLA SEROVAR: WHOLE CULTURE WITH ALUMINUM HYDROXIDE AND LIPOPOLYSACCHARIDE SUBUNIT WITH LIPID A

description: ABSTRACT It was compared the antibody response of sows immunized with two experimental vaccines produced with L.interrogans, serovar Canicola, strain LO-4, isolated in Brazil.One of the vaccines was the usual bacterin (whole culture inactivated with phenol and adjuvanted with aluminum hydroxide -WC-AlOH3) and the other one was a subunit vaccine produced with a lipopolysaccharide (LPS) fraction extracted from the bacteria outer envelop and with the lipid A, also extracted from the leptospira wall as adjuvant (LPS-MPLA). Experiment was as follows: group 1 (n = 11), not immunized control, group 2, (n = 11): two immunization with 30 days interval of LPS-MPLA vaccine and group 3 (n = 11): two immunization with 30 days interval of WC-AlOH3 vaccine All three groups were simultaneously immunized, independently of pregnancy stage. Both agglutinin and neutralizing post vaccination antibodies levels were measured respectively by the microscopic sera agglutination with live antigens test (MAT) and the in vitro leptospira growth inhibition test (GIT). Sera collections were performed each 30 days during four months after the first vaccination. Non vaccinated control group animals presented no agglutinating antibodies against Canicola serovar during the whole experiment. At 32 and 68 post vaccination days the agglutinating antibodies levels of group 2 (LPS-MPLA) were significantly higher than the observed in group 3 (WC AlOH3), respectively, p = 0.013 and p = 0.031. The differences observed in the growth inhibition antibodies titers of the two vaccines tested were not significant (p &gt; 0.05). Despite the peak of post-vaccination agglutinins have been registered at 68 days after first immunization, higher levels growth inhibition antibodies were detected at 30 days of first vaccination. Subunit vaccine presented the same immunogenic capacity for the production of neutralizing antibodies as the whole culture one.
DOI: 10.6084/m9.figshare.14288599
title: LISTERIA MONOCYTOGENES: OCCURRENCE IN DAIRY PRODUCTS AND ITS IMPLICATIONS IN PUBLIC HEALTH

description: ABSTRACT Listeria monocytogenes is the causative agent of listeriosis, a serious foodborne disease that promotes severe human infections with high mortality rates. Milk and byproducts are among the food products most often involved in the transmission of L. monocytogenes. Listeriosis mainly affects immunodepressed individuals, pregnant women, neonates and the elderly, thus emphasizing its opportunistic character and importance to public health. The present article presents a narrative and critical review concerning the risk to human health from the consumption of dairy products contaminated with L. monocytogenes. Also, a discussion is made on the factors that determine the contamination by L. monocytogenes in the production and distribution chain of milk and dairy products. The available data on the occurrence of L. monocytogenes in raw milk and dairy products in Brazil are also presented and evaluated, taking into consideration its potential for involvement in human listeriosis outbreaks. Additionally, this review indicates the main research and work areas needed for the prevention of L. monocytogenes contamination in dairy products.
DOI: 10.6084/m9.figshare.14288597
title: CULTIVATION OF PLEUROTUS SAJOR-CAJUIN DIFFERENT PASTEURIZED SUBSTRATES

description: ABSTRACT CULTIVATION OF PLEUROTUS SAJOR-CAJU IN DIFFERENT PASTEURIZED SUBSTRATES. This research was aimed at evaluating the viability of the Pleurotus sajor-caju crop using various residues generated in the region of Pelotas, state of Rio Grand do Sul, Brazil. The substrates were formulated with sugarcane bagasse, elephant grass and rice straw, separate and mixed, for a total of 7 treatments. The substrates were moistened for 24 hours, pasteurized at 100º C for 30 minutes, and then seeded with a 3% spawn of P. sajor-caju (PSC96/03) and placed into 1 kg polyethylene bags. The incubation was carried out at 25-28º C, for 25 days, and the harvest took place in two flushes after periods of 45 days each. The mushrooms were collected and weighed for the evaluation of the fresh mass (g), productivity (%) and biological efficiency (%). The results showed that the rice straw produces more in the first flush and the elephant grass more in the second, while the overall totals for fresh mass and productivity of both flushes showed that each of these materials was, separately, a viable substrate for the cultivation of P. sajor-caju mushrooms. The sugarcane bagasse proved to be the least indicated substrate for the cultivation of this mushroom by means of this technique.
DOI: 10.6084/m9.figshare.14288595
title: MICROBIOLOGICAL QUALITY OF “MINAS FRESCAL” CHEESE MARKETED IN SANTA HELENA, PR, BRAZIL

description: ABSTRACT From May 2008 to April 2009 the microbiological quality of 40 samples of artisan-made cheeses and cheeses inspected by the State Inspection Service and Federal in Santa Helena, state of Paraná, Brazil, was evaluated by quantifying the most probable number of thermotolerant coliforms, count of Staphylococcus spp., research of yeasts and molds, and the determination of the presence/absence of Salmonella spp. and Listeria monocytogenes. In regard to the presence of thermotolerant coliforms, 90% of the artisan-made samples and 55% of inspected samples did not meet the standards established by ANVISA under Resolution Nº 12 dated 2 January 2001. In regard to the count of Staphylococcus spp., 100% of the artisan-made samples and 25% of the inspected samples did not meet the legal standards. Salmonella spp. and L. monocytogenes were not isolated in 25 g of the product, showing that for these agents the cheese samples analyzed, both the artisan made as well as the inspected production, were within the established standards (absence in 25 g of the product). The results revealed that of all the samples analyzed, only 15% were in accordance with the standards established by ANVISA, with a high percentage of artisan-made (100%) and inspected cheese (70%) outside the legal limits. Therefore, health authorities should pay more attention to this sector, since such products may endanger the health of consumers.
DOI: 10.6084/m9.figshare.14288593
title: LEAF, STEM AND ROOT ABSORPTION OF PYMETROZINE AND FLONICAMID TO CONTROL THE COTTON APHID APHIS GOSSYPII GLOVER, 1877 (HEMIPTERA: APHIDIDAE)

description: ABSTRACT Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) is a cotton pest, and if not controlled can reduce yield and fiber quality. The discovery of new chemical groups of insecticides brings new perspectives to IPM; pymetrozine and flonicamid are examples of new launched pesticides. This study was carried out to evaluate the efficacy of these two insecticides to control A. gossypii when they were absorbed by cotton root, stem and leaves. The statistical design was a randomized factorial (2 x 3) with five replications in blocks. Each plot consisted of a pot with two plants, kept under greenhouse conditions, in 2005. We evaluated the population density of aphids on the leaves during sixteen days after treatment. The results show: (1) flonicamid has efficiency when absorbed by stem and leaves, (2) pymetrozine efficacy is through foliar uptake, (3) the root absorption do not provide satisfactory control for both insecticides, and, (4) both insecticides control the aphid-cotton when is applied on leaves.