cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/7f44a-trt17
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-85/rawdata/</p>atlas_30032.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-85/rawdata/atlas_30032.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30032_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-85/3dimage_88964/atlas_30032_full.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30032_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-85/3dimage_90284/atlas_30032_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.qjps37
title: Occurrence Download

description: A dataset containing 235972 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Antennaria dioica"
 ]
}

 The dataset includes 235972 records from 654 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0005723-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/x42yg-8cn36
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-101/rawdata/</p>atlas_30024.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-101/rawdata/atlas_30024.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30024_part51_2.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-101/3dimage_88983/atlas_30024_part51_2.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30024_full_1k.rec,  Reconstruction (Pixel Size 1.615 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-101/3dimage_90295/atlas_30024_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kykav-y4790
title: Exp phase S. acidocaldarius

description: Collected sm2017-12-21-x dataset on Caltech Krios
description: <strong>Tilt Series Date:</strong> 2017-12-21</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Sulfolobus acidocaldarius</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD for backprojection, tomo3d for SIRT</p>
<strong>Collaborators and Roles:</strong> Megan Dobro's stock of cells</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew cells to stationary phase overnight at 77 C in ATCC medium 1723 by shaking at ~180 rpm. Topped up H2O every 12-16 hr. Diluted cells in new medium to ~0.02 OD and grew till 0.05 OD.</p>
<strong>Sample Preparation:</strong> Kept cells on ice until freezing. Concentrated cells to ~5 OD. Added 3.5 µL of cells on glow discharged Cu finder grids and blotted for 2-3 sec from behind, manually.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-2/rawdata/</p>TS3.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-2/rawdata/TS3.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS3_4bin_SIRT90rot_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-2/3dimage_91665/TS3_4bin_SIRT90rot_DB.rec"
        > <i class="download icon"></i> Download </a></p>

        TS3_4bin_WBP_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 324.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-12-21-2/3dimage_91666/TS3_4bin_WBP_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6rrj7-44x06
title: LCV_Legionella pneumophila

description: THP1 cells were infected with Legionella wild type cells and thereafter phagosomes (LCV) were purified by sucrose density gradient method. Phagoseomes were fixed before shipment.
description: <strong>Tilt Series Date:</strong> 2017-04-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Phagosome isolation method as described by Joelle Gabay (Endnote).</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-34/rawdata/</p>LCV_wt_20170413ab0004.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-34/rawdata/LCV_wt_20170413ab0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        LCV_wt_20170413ab0004_part47_3.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-04-14-34/3dimage_83646/LCV_wt_20170413ab0004_part47_3.rec"
        > <i class="download icon"></i> Download </a></p>

        dga2017-04-14-34_slicer18444.png, , 856.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/dga2017-04-14-34_slicer18444.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0s3n8-fbx39
title: S. aureus RN6390 ΔESX

description: Staphylococcus aureus strain RN6390 ΔESX deletion mutant
description: <strong>Tilt Series Date:</strong> 2017-09-28</p>
<strong>Data Taken By:</strong> Georges Chreifi</p>
<strong>Species / Specimen:</strong> Staphylococcus aureus</p>
<strong>Strain:</strong> subsp. aureus str. Newman</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> etomo + manual tracking</p>
<strong>Collaborators and Roles:</strong> Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.</p>
<strong>Sample Preparation:</strong> 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-99/rawdata/</p>atlas_30038.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-99/rawdata/atlas_30038.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas_30038_part51_10.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-99/3dimage_88981/atlas_30038_part51_10.rec"
        > <i class="download icon"></i> Download </a></p>

        atlas_30038_full_1k.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-99/3dimage_90294/atlas_30038_full_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0z9v2-8jg35
title: BacillusSubtilis_328_4hr

description: This is Bacillus Subtilis tomograms PonA mutant strain 328 (obtained from Amit in Sigal Ben  .  Yehuda lab at the Hebrew university). The idea is to image bacterial nanotubes in Bacilus .  Subtilis.
description: <strong>Tilt Series Date:</strong> 2020-02-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> AB328, ΔponA ΔymdB amyE::ymdB Δhag</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates . (I will write the details on my Labjournal).  </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Samples were grown on the grids on agar plates for four hours. Detailed description is in the lab . journal. Yuxi helped in the sample preparation and data collection.</p>
<strong>Sample Preparation:</strong> Samples were taken from the agar plates and washed few times in PBS. Then they were blotted . and plunge-frozen.</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-7/rawdata/</p>Bsubtilis_328_4hr_conventional2.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-7/rawdata/Bsubtilis_328_4hr_conventional2.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_328_4hr_conventional2_part31_4.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-7/3dimage_110654/Bsubtilis_328_4hr_conventional2_part31_4.rec"
        > <i class="download icon"></i> Download </a></p>

        Bsubtilis_328_4hr_conventional2.mrc.mdoc,  24.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-7/rawdata/Bsubtilis_328_4hr_conventional2.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z3bjs-vhd42
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-72/rawdata/</p>Ecoli_CONjugation34.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-72/rawdata/Ecoli_CONjugation34.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation34_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-72/3dimage_107147/Ecoli_CONjugation34_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation34.mrc.mdoc,  50.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-72/rawdata/Ecoli_CONjugation34.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fd978-cwj24
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-28/rawdata/</p>Ecoli_Conjugation2.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-28/rawdata/Ecoli_Conjugation2.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_Conjugation2_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-28/3dimage_107057/Ecoli_Conjugation2_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_Conjugation2.mrc.mdoc,  50.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-28/rawdata/Ecoli_Conjugation2.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s38gd-vvm76
title: Ecoli_Cojugation

description: These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same  .  cells described in the Science paper (Direct visualization of horizontal gene transfer). The .  cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. .  Targets were imaged with cryo-ET randomly.
description: <strong>Tilt Series Date:</strong> 2019-02-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were sent to the Jensen lab from the lab of Ariel Lindner in Paris. These are the same . cells used in their Science paper (direct visualization of horizontal gene transfer).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> ANB 35 were grown in LB while ANB24 (Recipient) in M9. I followed the same protocol . described in the Science paper described above.</p>
<strong>Sample Preparation:</strong> The donor and recipient cells were mixed on the EM grid, incubated in the vitrobot chamber with . 100% humidity and then blotted and plunge frozen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-90/rawdata/</p>Ecoli_CONjugation50.mrc,  Tilt Series (Pixel Size 0.541 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-90/rawdata/Ecoli_CONjugation50.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation50_full.rec,  Reconstruction (Pixel Size 2.164 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-90/3dimage_107182/Ecoli_CONjugation50_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Ecoli_CONjugation50.mrc.mdoc,  49.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-02-28-90/rawdata/Ecoli_CONjugation50.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>