cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2010.877
title: Genetic characterization of porcine epidemic diarrhea virus (PEDV) isolates from Southern Vietnam during 2009-2010 outbreaks

description: Porcine epidemic diarrhea virus (PEDV) spike (S) protein and membrane (M) protein genes are believed to have genetic variation. The heterogeneity in those genomic sequences has been reported and is known essentially for the diverse PEDV pathogenicity. Eight southern Vietnamese PEDVs collected from watery diarrhea piglets of various farms of the recent emerging outbreaks (2009-2010) were sequenced and analyzed. The results revealed high homology of the partial S gene of the current isolates at 98.9-100% and 99.7-100% identity of full M gene among these isolates despite dividing into two clades of different provincial origins. It should be note that the Vietnamese PEDVs contained high differences on nucleotide sequence of partial S gene with other reference isolates in Europe (Br1/87, CV777) and Korea (Spk1, Chinju99, DR13 and KNU-0801). The phylogenetic relationship of both partial S and M protein genes indicated that the current Vietnamese PEDVs were in the same cluster with the recent isolates from China (JS-2004-2 and DX), Thailand (07NP01, 08NP02 and 08CB01) and Korea (KNU-0802 and CPF299). The results suggested that these Vietnamese PEDV isolates might have originated from the same Chinese ancestor and they have been undergoing genetic variation and forming a new PEDV genotype in Vietnam.
DOI: 10.14457/cu.the.2010.878
title: Safety stock calculation for jewelry industries based on consumption forecast by artificial neural network

description: To present the safety stock calculation based on consumption of components in the jewelry business was investigated using the forecasting capability of an Artificial Neural Network (ANN). Generally, this business also has links with fashion; therefore, rapid change in the fashion industry makes the forecasting situation more complicated. The demand fluctuates with customer requirements and high competition in the marketplace. Factors such as late delivery and bad component quality can cause shortages of components. To prevent shortages, provide support to supply management and enhance customer satisfaction, an ANN is utilized for consumption forecast. Safety stock is the way to protect against shortages. Safety stock is created when a company either orders before an order is needed or orders more than the expected demand. In the contrast, keeping less safety stock is important for decreasing cost and increasing interests for a firm. In practice, business has calculated safety stock based on their experiences, non theoretical support, and leads for retaining component in the warehouse. Hence, safety stock is essential to protect against the fluctuation of unexpected demand and supply. The central focus of this paper is to enhance the forecast on safety stock accuracy in the jewelry business. The artificial neural network is employed as a tool for the prediction. It is assumed that the accurate consumption leads to the accuracy in safety stock calculation.
DOI: 10.14457/cu.the.2010.879
title: Taxonomy of xylanolytic bacteria and characterization of xylanase from selected strains

description: Seventy isolates of xylanase-producing bacteria were isolated from 45 samples of soil collected in Thailand. These bacteria were divided into sixteen groups based on their phenotypic and chemotaxonomic characteristics including 16s rRNA gene sequences of the representative strains. Sixty-one strains were Gram-positive rods belonged to Bacillus 25 isolates, Paenibacillus 24 isolates, Cohnella 4 isolates, Isoptericola and Jonesia each of 2 isolates, Microbacterium 3 isolates and Nocardioides 1 isolate. Each isolate of Gram-negative rods, was belonged to Acinetobacter, Aeromonas, Blastobacter, Ensifer, Pseudomonas, Sphingobacterium, Sphingomonas, Stenotrophomonas and Zobellella. They were identified as Bacillus licheniformis 4 isolates, Paenibacillus barengoltzii 3 isolates; each of 2 isolates was B. subtilis subsp. subtilis, B. niabensis, B. cereus, Isoptericola variabilis, Jonesia denitrificans and Microbacterium natoriense; and each of 1 isolate was B. nealsonii, P. macerans, P. timonensis, P. montaniterrae, P. dendritiformis, Nocardioides simplex, Acinetobacter junii, Aeromonas enteropelogenes, Ensifer adhaerens, Pseudomonas stutzeri, Stenotrophomonas maltophilia and Zobellella denitrificans. In addition, the novel species of Bacillus 2 isolates, Paenibacillus 12 isolates, Cohnella 4 isolates and each isolate of Microbacterium, Blastobacter, Sphingobacterium, Sphingomonas were identified based on the differential phenotypic, chemotaxonomic characteristics and 16S rRNA gene sequences similarity (96.0-98.8%). The Gram-positive rod-shaped, spore forming bacteria, Paenibacillus thailandensis sp. nov., P. nanensis sp. nov., P. xylanisolvens sp. nov., Cohnella thailandensis sp. nov, C. xylanilytica sp. nov. and C. terrae sp. nov. were proposed. Bacillus sp. P2-3 produced the highest xylanase activity, when compared to other isolates. Thus, P2-3 was selected for further study. Corn cob was found to be the most preferred substrate for xylanase production. After optimization of medium composition and pH, the yield was increased about 2 times when compared with the initial medium. The partially purified xylanase from P2-3 had molecular weight of 17.7 kDa. The enzyme had a maximal activity at 60°C and pH 6. Stability remained more than 50% at 30–40°C and pH 3-11. The xylanase activity was activiated by the addition of 1 mM Ca²⁺, Mg²⁺, Mn²⁺, DTT, and β-Me. In contrast, the xylanase activity was slightly inhibited by Fe²⁺, PMSF and SDS. The partially purified xylanase had the highest hydrolytic activity toward Oat spelt xylan, but no activity toward β-glucan, carboxymethylcellulose and pectin.
DOI: 10.14457/cu.the.2010.880
title: Binding properties and crystallization of crustinPm1 and crustinPm7 from tha black tiger shrimp penaeus monodon

description: Crustin is one of several antimicrobial peptides (AMPs) in innate immune system of crustacean. In previous study, crustinPm1 strongly inhibited gram-positive bacteria, whilst crustinPm7 acted against both gram-positive and gram-negative bacteria. The aim of this study is to investigate (1) Binding properties of rcrustinPm1 and rcrustinPm7 to bacterial cells and cell wall components, lipoteichoic acid (LTA) and lipopolysaccharide (LPS) (2) Induction of bacterial agglutination by rcrustinPms (3) Inner membrane permeabilization (4) Effects of rcrustinPms on bacteria cells and (5) Crystallization of rcrustinPms. In this study, the recombinant crustinPm1 and crustinPm7 were produced in Escherichia coli and purified under denaturing condition. The purified rcrustinPms were then renatured and used in antimicrobial activity test. Binding study suggested that both crustin isoforms can bind to both gram-positive and gram-negative bacteria, as well as cell wall components LTA and LPS. CrustinPms can induce bacterial agglutination in some strains of bacteria. They can also change inner membrane permeability of E. coli strain MG1655, resulting in cytoplasmic -galactosidase release. Scanning Electron Microscopy (SEM) revealed physical change on cell surface of Staphylococcus aureus, Vibrio harveyi and E. coli treated with rcrustinPm7. Recombinant crustinPm1; however, can cause physical changes on cell surface of S. aureus and E. coli only. Crystallization experiments were carried out. Recombinant crustinPm1 was crystallized in 0.2 M Lithium citrate tribasic tetrahydrate, 20% PEG 3350, pH 8.4. However, no crytallization condition for rcrustinPm7 was found.
DOI: 10.14457/cu.the.2010.881
title: Synthesis and fluorescent properties of dendritic polyelectrolyte fluorophores

description: Water soluble fluorophores containing multiple fluorogenic moieties are of interests for chemical analysis and highly sensitive medical diagnosis. The fluorophores with controllable numbers of fluorogenic units can provide good mechanistic understanding and experimental reproducibility. In this thesis, a series of nine variously charged dendritic fluorophores (charges: 6C⁻, 3C⁻, 2C⁻N⁰, C⁻2N⁰, 2C⁻N⁺, 2C⁰N⁺, C⁰2N⁺, 3N⁺, and 6N⁺) composed of exact numbers of the para-phenyleneethynylene (PPE) fluorogenic branches and anionic carboxylate or cationic ammonium peripheral groups are synthesized via Sonogashira coupling. The first generation anionic dendrimer, 6C⁻ exhibits a highly selective fluorescence quenching by Hg²⁺ ions with quenching efficiency Ksv of 33,700 M⁻¹ in the presence of Triton X-100 surfactant. The nine fluorophores are assembled into an array for protein analysis. Using principal component analysis (PCA) and factorial discriminant analysis (FDA), the optimum detection wavelength is located at 500 nm and the number of sensing elements is reduced from nine to two (C⁰2N⁺ and 3C⁻) with 100% discriminating accuracy. The cellulose nanofiber mats doped with 3C⁻ fluorophore are fabricated by electrospinning technique. The mats can be applied as a reusable solid-state fluorescent sensing device for metalloproteins such as hemoglobin which shows high Ksv of 1.7×10⁶ M⁻¹. The findings in this thesis work have established a new interesting class of fluorophores useful for sensing applications in aqueous media.
DOI: 10.14457/cu.the.2010.882
title: Solid phase extraction of lead in water using triethylenetetramine-modified resin for determination by UV-visible and atomic spectrometries

description: Polystyrene-divinylbenzene contained triethylenetetramine (TETA-PS-DVB) was successfully prepared by two-step reaction and employed as sorbent in solid phase extraction (SPE) of Pb(II) before its determination by UV-visible spectrometry and flame atomic absorption spectrometry (FAAS). The sorption performance of Pb(II) in single metal solution was similar to that obtained by binary metal solutions (Pb(II) with Co(II), Cr(II), Cu(II), Ni(II), and Zn(II)) indicating the high selectivity of the sorbent. Under optimal conditions, 60 minutes were utilized for adsorption of Pb(II) on the sorbent in batch method and the kinetics of sorption fitted well with pseudo-second order rate. The maximum sorption capacity calculated from Langmuir adsorption isotherm was found to be 4.04 µmol/g. The suitable desorption solution and elution time for determination by FAAS were 10% (v/v) HNO3 and 30 minutes, respectively. While, the determination by UV-visible spectrometry was successful using saturated dithizone in 7% (v/v) triton X-100 solution and controlling the pH at 5.5 by acetate buffer solution. The method could be applied for the extraction of Pb(II) in cement-base leachate with the percentage recoveries of 81 to 84 and 81 to 86 for determination by FAAS and UV-visible spectrometry, respectively. The relative standard deviation for determination by FAAS and UV-visible spectrometry were 2.19% and 2.58%, respectively. The extraction in column method showed unsatisfied low extraction percentage suggesting that the solid phase was not suitable for using in column mode. The used solid phase was unable to be reused.
DOI: 10.14457/cu.the.2010.883
title: An incentive compatible mechanism for booth auction / Puchit Sariddichainunta

description: The winner determination problem (WDP) for a single object auction is a relatively easy problem to solve using the greedy algorithm. It can be formulated and solved using the MIP optimization solver. In this thesis, we applied WDP to solve a booth auction which is one of the nonidentical multiple-object auctions known to be NP-hard. Formulation of the winner determination model for a linear arrangement of a multiple-object auction is explained in this study. Moreover, this research improves the algorithm from the study of Rothkopf et al (1998) having polynomial time complexity. Finally, the comparison of a running time exhibits the advantage of our proposed algorithm. The simulation results are discussed.
DOI: 10.14457/cu.the.2010.884
title: Factors associated with diarrhea among under-five years old children in Banten province Indonesia : a secondary analysis of Indonesian national socio-economic survey 2007 and basic health research 2007

description: This study was a secondary analysis of two surveys in Indonesia, Indonesian National Socio-economic Survey 2007 and Basic Health Research 2007. Related variables from these surveys were used to examine factors associated with diarrhea among under-five years old children in Banten Province, Indonesia. Primary data sources were consist of household economic, individual economic and health information. A two stage sampling was done using probability proportional to the number of households in district/city. There were 303 census blocks selected by random sampling which each block consist of 150 households. Then, from each census block 16 households randomly selected as the sample of household. Each household member became respondent of primary data collection. This study used the data of 1655 children under-five years old along with data of their mothers and households. Bivariate analysis was done with Pearson’s chi-square test, crude odds ratio for risk estimation, and multivariate analysis being done using binary logistic regression. The prevalence of diarrhea among under-five years children was 18.9%. The highest risk was in children age 6-11 months, lower education mother (p-value 0.001) and without gender influences. Mother’s defecation place and hand washing behavior has strong association with diarrhea in children (p-value &lt; 0.001 and &lt;0.001). Teenage mothers were found have high risk of having children with diarrhea (p-value 0.042) and also high proportion of never practiced hand washing with soap (15.8%). Using unsafe drinking water source and not good physical quality of drinking water were associated with diarrhea in children (p-value &lt; 0.001 and 0.005). As well as the household that shared in the use of drinking water source and latrine, use open water container, open liquid drainage channel and did not use septic tank for feces landfills. Percentage of children’s diarrhea was higher in rural area, but after controlling other variables, urban area has 0.6 more likely develop diarrhea in children. Child health care, such as exclusive breast feeding, food and vitamin A supplementation, measles immunization were not enough to prevent the under-five years old children suffer from diarrhea. Health educations, especially for teenage mother, and promote the use of safe drinking water source, latrine and household sanitations are recommended. Specific intervention should be implemented for rural area with difficulties to get drinking water source. Longitudinal study is needed to identify confounding factors, causal relationships and seasonal differences in the epidemiology of diarrhea.
DOI: 10.14457/cu.the.2010.885
title: Outcomes of pharmacist counseling on outpatients receiving warfarin at Samutprakarn Hospital

description: This one group pre-test and post-test study was designed to assess the outcome of pharmacist counseling on outpatients receiving warfarin therapy by compare patients’ knowledge, compliance, International Normalized Ratio (INR), bleeding and thromboembolic events, and cost for hospitalization and ER visits between pre- and post- pharmacist counseling from January 2008 to January 2009. Samples were outpatients who received long-term warfarin therapy at Samutprakarn Hospital during the study period and have been followed up at warfarin clinic not less than 3 visits. Data were collected on 132 outpatients from an intervention day until three follow-up at warfarin clinic. Patients’ knowledge of warfarin therapy increased after receiving pharmacist counseling service and maintained until a third visit. Patients’ compliance to warfarin regimens was improved in the second visit. Percentage of patients whose INR within therapeutic range was increase after receiving pharmacist counseling (22.1% VS 73.5%). Percentage of patients whose INR lower and higher than therapeutic range were decreased after receiving pharmacist service (53.8% VS 14.1%, 24.1% VS 12.4%). Bleeding and thromboembolic events at post pharmacist counseling were reported lower than pre-pharmacist counseling (3.6% VS 1.7%, 2.2% VS 1.6%). A lower event of hospitalizations after counseling service (10 events VS 4 events) produced cost saving of 242,357.52 baht. From this study showed that pharmacist counseling could improved patients’ knowledge, compliance, anticoagulation control, reduced bleeding and thromboembolic events and annually saved cost in reduced ER visits and hospitalizations.
DOI: 10.14457/cu.the.2010.886
title: Technical and scale efficiency of government district base hospitals in Srilanka

description: To study of technical and scale efficiency and to observe the factors affecting on efficiency in government district base hospitals in Sri Lanka. Data envelopment analysis or DEA with Tobit regression analysis were used to find solutions. 168 decision making units or DMUs were evaluated concerning 56 hospitals for three years (2006, 2007, and 2008). Initial relative efficiency results show that technical efficiency level has ranged between 0.383 and 1.0, average score level was 0.818 while the scale efficiency level has ranged between 0.412 and 1.0, its’ average score was 0.905. Moreover, 71% of DMUs has been operating at technical inefficiently while 83% were scale inefficiently. Among scale inefficient DMUs, 32% DMUs show increasing returns to scale and 68% of DMUs show decreasing returns to scale. After implementation of health master plane 2007-2016, there is an increasing trend on technical and scale efficiency. Secondly, Tobit regression analysis found that bed occupancy ratio positively related with technical efficiency [.299, p = .017] and bed physician ratio negatively associate with technical efficiency [.0008, p = .044] and also hospitals belong to MoH are technically efficient rather than provincial hospitals. When increase the ratio of nurses’ physician and allied health personnel physicians, technical efficiency will be increased. However, these factors are statistically insignificant. Large hospital (Type A) technically and scale efficient than smaller hospitals (Type B). Number of admissions tended to increase scale efficiency [-1.34E-06, p = .00]. Majority of institutions are operating at decreasing returns to scale. So, the reducing bed size from those institutions is recommended. It will be directed them towards efficiency because of the bed occupancy ratio highly positively associated as well as the bed physician ratio related with it.
DOI: 10.14457/cu.the.2010.888
title: Hydraulic fracturing strategy in gas condensate reservoirs

description: Gas condensate reservoirs usually exhibit complex flow behaviors. The gas condensate reservoir is initially gas at the reservoir condition. Liquid forms in the reservoir when the bottomhole pressure drops below the dew point pressure. The accumulated condensate in the vicinity of the well bore causes a blockage effect, reduces the gas effective permeability, and causes reduction in well productivity and recovery of heavy components at the surface. Hydraulic fracturing is a well known and common practice to improve the well productivity, especially in tight gas condensate reservoirs. Horizontal well with hydraulic fractures can achieve significant productivity improvements over a non-fractured horizontal well. Many horizontal wells have been drilled in gas condensate reservoirs, but a few of them was hydraulically fractured. The main objective of this paper is to study hydraulic fracturing for horizontal wells in gas condensate reservoirs using reservoir simulation model to obtain better understanding of its effect on gas and condensate recovery. Effect of different hydraulic fracture design parameters on the performance of hydraulically fractured horizontal well was studied. These parameters are fracture spacing, fracture half-length, fracture width, fracture permeability and number of fracture. The results found in this study show that fracturing the well significantly decreases gas production time. Hydraulic fracturing reduces the pressure drawdown, leading to less liquid condensate dropout near the wellbore and more condensate produced at surface.
DOI: 10.14457/cu.the.2010.889
title: Knowledge, attitude and practice on disposal of sharp waste, used for home management of type-2 diabetes mellitus in New Delhi, India

description: This cross-sectional study explored the present practices on household sharp waste management among type 2 diabetics in Delhi, India. A self-administered questionnaire was used to collect and describe the socio-demographic parameters, diabetes home management characteristics, knowledge, attitude, practices and influencing factors towards household sharp waste management. Twenty diabetic clinics in Delhi enrolled a total of 303 type 2 diabetics, who were above 18 years of age and administering insulin at home. Chi-square and correlation were used to assess the associations of factors with level of practice on sharp management at home. Only 3.6% of respondents stated correct knowledge about safe disposal method. The study showed that 43 (14.2%) of responders had any kind of information on sharp waste management from their healthcare provider. Regarding practices, (84.4%) of respondents threw away their sharps in household garbage bins every day. Influencing factors like education form healthcare provider and pharmacist/friends were significantly associated with good disposal practices by home insulin users (p-value
DOI: 10.14457/cu.the.2010.890
title: Solvent extraction at high temperture for determination of phthalate esters in cellulose lacquers

description: Cellulose lacquers is one of the paint used in surface coating furniture and automobile. Phthalate esters are used as the plasticizer in this paint for improves brittle property of film. However, they were suspected to have carcinogenic and estrogenic properties. Reflux is the most commonly used as sample preparation technique for phthalate esters determination in plastic samples, It usually required high amount of sample and solvent. In this study, we aimed to study a sample preparation technique, which required less solvent and extraction time. The solvent extraction at high temperature was investigated for the extraction of phthalate esters from cellulose lacquers paints and determined by gas chromatography. Three phthalate esters, dibutyl phthalate (DBP), di(2-ethylhexyl phthalate) (DEHP) and diisodecyl phthalate (DIDP) were used as solute probes. The effects of types of solvent, ratio of solvent mixtures, ratio between sample and extracting solvent, speed of mechanical stirrer, extraction time and temperature on the extraction efficiency were studied. The ratio of 1:1 between sample to n-hexane with the extraction time of 20 min, extraction temperature of 40ºC and mechanical stirrer speed of 45 rpm were found to be the suitable condition. The recoveries of DBP, DEHP and DIDP at 25ºC were lower than at 40ºC and in range 70-90%. The method of quantification of DBP, DEHP and DIDP were 4.30, 1.10 and 0.70 g/L an relative standard deviation were 12.64, 15.08 and 13.97%, respectively. This studied method was also applied to analyze phthalate esters content in commercialized cellulose lacquer.