DOI: 10.5061/dryad.mcvdnck9x
title: Concentrations of estrogenic chemicals in fish and shellfish tissues from Puget Sound, WA

description: The Washington Department of Fish and Wildlife Toxics Biological
 Observation System (TBiOS) team conducts biennial surveys to monitor the
 status and trends of toxic contaminants in indicator species throughout
 Puget Sound, WA, USA. This dataset includes concentrations of estrogenic
 contaminants measured in whole body, fillet, or bile samples from juvenile
 Chinook salmon (Oncorhynchus tshawytscha), adult resident Chinook salmon,
 Pacific herring (Clupea pallasii), English sole (Parophrys vetulus), and
 bay mussels (Mytilus trossulus). These samples were collected as part of
 TBiOS' ongoing surveys and were opportunistically analyzed for
 contaminants of emerging concern.
description: <strong>Sample collection and preparation</strong>:
 Fish were collected by beach seine, gill net, bottom trawl or from a
 recreational or commercial test fishery between 2013 and 2018. English
 sole bile samples were collected immediately after collection and frozen
 at -20°C until analysis. After collection, fish were frozen and stored at
 -20C or -80C until processed. Fish were processed as whole bodies or
 edible fillet samples, depending on species. Composite samples were
 created by combining tissues from 2-20 fish and homogenized into a single
 representative sample, frozen and stored at -20°C until
 analysis.  Mussel samples were from 2012-2013 and
 2019-2020 surveys. Cultured, pre-reproductive native bay mussels sourced
 from a commercial aquaculture facility were caged and deployed at the mean
 lower low water level in multiple nearshore sites in Puget Sound for 3
 months during the winter. Retrieved mussels from each site were cleaned by
 deionized water to remove external debris and sorted to remove dead
 mussels. Soft tissues (including the adductor muscle) were removed from 32
 live mussels, homogenized into a single composite sample for each site,
 frozen and stored at -20°C until analysis. 
 <strong>Analytical chemistry: </strong>Whole body and
 fillet tissue samples were analyzed for alkylphenols, pharmaceuticals and
 personal care products, and per- and polyfluoroalkyl substances at SGS
 AXYS Analytical Laboratory (Sydney, BC). English sole bile samples were
 analyzed for a suite of estrogenic chemicals at the National Oceanic and
 Atmospheric Administration's Northwest Fisheries Science Center as
 described in Da Silva et al. (2013). 
description: # Concentrations of estrogenic chemicals in fish and shellfish tissues
 from Puget Sound, WA Dataset DOI:
 [10.5061/dryad.mcvdnck9x](10.5061/dryad.mcvdnck9x) ## Description of the
 data and file structure ### Files and variables The following files
 contain contaminant concentration data for fish and shellfish samples
 necessary to replicate analyses in Faber et al., (in review), examining
 the estrogenic activity of mixtures that biota are exposed to in the
 Salish Sea. Descriptions of analytical lab flags, blank adjusted
 concentrations and blank adjusted detection limits apply across all three
 files.  #### File: WDFW\_eEDC\_fish\_bile\_data.csv **Description:** This
 CSV file contains contaminant concentration data for fish bile samples
 ##### Variables * SAMPLE_ID: Unique sample identifier * SiteName: Sample
 location identifier * Species: Common species name * Species_2: Scientific
 species name * Tissue_type: Sample tissue type * Lab: Analytical chemistry
 lab where tissue was analyzed * COMPOUND: Compound name * CAS_NO: Chemical
 Abstract Services (CAS) Number * CONC_FOUND: Measured concentration *
 UNIT: Units for measured concentration and detection limit #### File:
 WDFW\_eEDC\_fish\_tissue\_data.csv **Description:** This CSV file contains
 contaminant concentration data in fish muscle and whole body samples #####
 Variables * SAMPLE_ID: Unique sample identifier * COMPOUND: Compound name
 * CAS_NO: Chemical Abstract Services (CAS) Number * CONC_FOUND: Measured
 concentration * UNIT: Units for measured concentration and detection limit
 * DETECTION_LIMIT: Detection Limit * LAB_FLAG: Lab Flags (described below)
 * ADJ_CONC_FOUND: Blank Adjusted Concentration (described below) *
 ADJ_DL: Blank Adjusted Detection Limit (described below) #### File:
 WDFW\_eEDC\_mussel\_data.csv **Description:** This CSV file contains
 contaminant concentration data in bay mussel whole body samples #####
 Variables * SAMPLE_ID: Unique sample identifier * COMPOUND: Compound name
 * CAS_NO: Chemical Abstract Services (CAS) Number * CONC_FOUND: Measured
 concentration * UNIT: Units for measured concentration and detection limit
 * DETECTION_LIMIT: Detection Limit * LAB_FLAG: Analytical lab Flags
 (described below) * ADJ_CONC_FOUND: Blank Adjusted Concentration
 (described below) * ADJ_DL: Blank Adjusted Detection Limit (described
 below) ### **Analytical lab flag descriptions:**  | Flags | Description |
 | :---- |
 :--------------------------------------------------------------------------------------------------------------------- | | D | Dilution data | | J | Concentration less than LMCL | | B | Found in sample blank | | N | Recovery not within limits | | V | Surrogate recovery not within limits | | G | Lock mass interference | | NA\* | Not available | | NQ\* | Data not quantifiable | | OLR\* | Outside linear calibration range | | H | Estimate | | max\* | Concentration is estimated maximum value | | U\* | Not detected | | K\* | Peak detected but did not meet quantification criteria; result represents the estimated maximum possible concentration | | P | Adjusted detection limit due to blank detections (see below for details) | \* Chemical detections with these flags were removed from analysis. #### Blank detections were noted in the following compounds: | Chemical Name | CAS Number | Number of blanks detections in lab blanks | | :----------------------------------- | :---------- | :---------------------------------------- | | 4-Nonylphenol monoethoxylate (NP1EO) | 104-35-8 | 3/9 | | 4-Nonylphenol | 104-40-5 | 10/10 | | Clinafloxacin | 105956-97-6 | 1/13 | | DEET | 134-62-3 | 9/13 | | Thiabendazole | 148-79-8 | 0/13 | | 4-Nonylphenol diethoxylate (NP2EO) | 20427-84-3 | 3/9 | | Anhydrochlortetracycline \[ACTC] | 4497-08-9 | 1/13 | | Theophylline | 58-55-9 | 0/13 | | 4-n-Octylphenol | 1806-26-4 | 4/11 | #### Blank correction methods * Blank corrections were made for chemicals with blank detections in 3 or more samples * For chemicals with blank detections, detection limit was adjusted to a new detection limit based on blank distribution. All concentrations below the detection limit are reported as 1/2 the adjusted detection limit. * Adjusted detection limits were added to the ADJ_DL column * All chemical detections and blank corrected detections were added to ADJ_CONC_FOUND column * All chemicals with an adjusted detection limit include the P flag * U flag was added to all chemical concentrations which fall below the ajusted detection limit, 1/2 the adjusting reporting limit was used for those chemicals