cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6045041.v1
title: Effect of pre-exercise carbohydrate availability on fat oxidation and energy expenditure after a high-intensity exercise

description: The aim of this study was to test the hypothesis that reduced pre-exercise carbohydrate (CHO) availability potentiates fat oxidation after an exhaustive high-intensity exercise bout. Eight physically active men underwent a high-intensity exercise (∼95% V̇O2max) until exhaustion under low or high pre-exercise CHO availability. The protocol to manipulate pre-exercise CHO availability consisted of a 90-min cycling bout at ∼70% V̇O2max + 6 × 1-min at 125% V̇O2max with 1-min rest, followed by 48 h under a low- (10% CHO, low-CHO availability) or high-CHO diet (80% CHO, high-CHO availability). Time to exhaustion was shorter and energy expenditure (EE) lower during the high-intensity exercise in low- compared to high-CHO availability (8.6±0.8 and 11.4±1.6 min, and 499±209 and 677±343 kJ, respectively, P&lt;0.05). Post-exercise EE was similar between low- and high-CHO availability (425±147 and 348±54 kJ, respectively, P&gt;0.05), but post-exercise fat oxidation was significantly higher (P&lt;0.05) in low- (7,830±1,864 mg) than in high-CHO availability (6,264±1,763 mg). The total EE (i.e., exercise EE plus post-exercise EE) was similar between low- and high-CHO availability (924±264 and 1,026±340 kJ, respectively, P&gt;0.05). These results suggest that a single bout of high-intensity exercise performed under low-CHO availability increased post-exercise fat oxidation, and even with shorter exercise duration, both post-exercise EE and total EE were not impaired.
DOI: 10.6084/m9.figshare.6045032
title: Physicochemical, structural and thermal properties of oxidized, acetylated and dual-modified common bean (Phaseolus vulgaris L.) starch

description: Abstract Common beans are rich in protein and complex carbohydrates that are valuable for the human diet. Starch is the most abundant individual component; however, in its native form it has limited applications and modifications are necessary to overcome technological restrictions. The aim of this study was to evaluate the influence of oxidation, acetylation and dual-modification (oxidation-acetylation) on the physicochemical, structural and thermal properties of common bean starch. The degree of substitution of the acetylated starches was compatible with food use. Fourier transform infrared spectra confirmed the acetylation of the bean starch, with a peak at 1,735cm-1. The granules of the bean starch were oval to spherical in shape, with no differences between the native and modified samples. Typical C-type diffraction of legume starches was found. The modified samples showed a reduced relative crystallinity and lower enthalpy change of gelatinization. The oxidized starch showed the highest peak viscosity, hardness, and gel adhesiveness due to the presence of functional groups. An increase in solubility and swelling power was observed, and the oxidized-acetylated starch presented the highest values. The properties of the modified bean starches made them suitable for application in breaded/battered foods, mainly due to improved textural attributes.
DOI: 10.6084/m9.figshare.6045032.v1
title: Physicochemical, structural and thermal properties of oxidized, acetylated and dual-modified common bean (Phaseolus vulgaris L.) starch

description: Abstract Common beans are rich in protein and complex carbohydrates that are valuable for the human diet. Starch is the most abundant individual component; however, in its native form it has limited applications and modifications are necessary to overcome technological restrictions. The aim of this study was to evaluate the influence of oxidation, acetylation and dual-modification (oxidation-acetylation) on the physicochemical, structural and thermal properties of common bean starch. The degree of substitution of the acetylated starches was compatible with food use. Fourier transform infrared spectra confirmed the acetylation of the bean starch, with a peak at 1,735cm-1. The granules of the bean starch were oval to spherical in shape, with no differences between the native and modified samples. Typical C-type diffraction of legume starches was found. The modified samples showed a reduced relative crystallinity and lower enthalpy change of gelatinization. The oxidized starch showed the highest peak viscosity, hardness, and gel adhesiveness due to the presence of functional groups. An increase in solubility and swelling power was observed, and the oxidized-acetylated starch presented the highest values. The properties of the modified bean starches made them suitable for application in breaded/battered foods, mainly due to improved textural attributes.
DOI: 10.6084/m9.figshare.6045026
title: Soil physical indicators of management systems in traditional agricultural areas under manure application

description: ABSTRACT Studies of the successive application of manure as fertilizer and its combined effect with long-term soil management systems are important to the identification of the interdependence of physical attributes. The aim of this study was to evaluate changes in the physical properties of a Rhodic Kandiudox under management systems employing successive applications of pig slurry and poultry litter, and select physical indicators that distinguish these systems using canonical discriminant analysis (CDA). The systems consisting of treatments including land use, management and the application time of organic fertilizers are described as follows: silage maize under no-tillage (NT-M7 years); silage maize under conventional tillage (CT-M20 years); annual pasture with chisel plowing (CP-P3 years); annual pasture with chisel plowing (CP-P15 years); perennial pasture without tillage (NT-PP20 years); and no-tillage yerba mate (NT-YM20 years) and were compared with native forest (NF) and native pasture (NP). Soil samples were collected from the layers at the following depths: 0.0-0.05, 0.05-0.10, and 0.10-0.20 m, and were analyzed for bulk density, porosity, aggregation, flocculation, penetration resistance, water availability and total clay content. Canonical discriminant analysis was an important tool in the study of physical indicators of soil quality. Organic fertilization, along with soil management, influences soil structure and its porosity. Total porosity was the most important physical property in the distinction of areas with management systems and application times of manure for the 0.0-0.05 and 0.10-0.20 m layers. Soil aeration and micropores differentiated areas in the 0.05-0.10 m layer. Animal trampling and machinery traffic were the main factors inducing compaction of this clayey soil.
DOI: 10.6084/m9.figshare.6045026.v1
title: Soil physical indicators of management systems in traditional agricultural areas under manure application

description: ABSTRACT Studies of the successive application of manure as fertilizer and its combined effect with long-term soil management systems are important to the identification of the interdependence of physical attributes. The aim of this study was to evaluate changes in the physical properties of a Rhodic Kandiudox under management systems employing successive applications of pig slurry and poultry litter, and select physical indicators that distinguish these systems using canonical discriminant analysis (CDA). The systems consisting of treatments including land use, management and the application time of organic fertilizers are described as follows: silage maize under no-tillage (NT-M7 years); silage maize under conventional tillage (CT-M20 years); annual pasture with chisel plowing (CP-P3 years); annual pasture with chisel plowing (CP-P15 years); perennial pasture without tillage (NT-PP20 years); and no-tillage yerba mate (NT-YM20 years) and were compared with native forest (NF) and native pasture (NP). Soil samples were collected from the layers at the following depths: 0.0-0.05, 0.05-0.10, and 0.10-0.20 m, and were analyzed for bulk density, porosity, aggregation, flocculation, penetration resistance, water availability and total clay content. Canonical discriminant analysis was an important tool in the study of physical indicators of soil quality. Organic fertilization, along with soil management, influences soil structure and its porosity. Total porosity was the most important physical property in the distinction of areas with management systems and application times of manure for the 0.0-0.05 and 0.10-0.20 m layers. Soil aeration and micropores differentiated areas in the 0.05-0.10 m layer. Animal trampling and machinery traffic were the main factors inducing compaction of this clayey soil.
DOI: 10.6084/m9.figshare.6045023
title: Optimizing functional properties of perilla protein isolate using the response surface methodology

description: Abstract Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters of perilla protein isolate (PPI), which has functional properties. A central composite experimental design was applied to investigate the effects of three factors (ultrasonic power, extraction time, and liquid-to-solid ratio) on six responses: protein yield, water solubility index (WSI), water absorption capacity (WAC), oil holding capacity (OHC), emulsifying activity (EA), and foaming ability (FA). Based on the RSM results, the optimum conditions were an ultrasonic power of 206 W, and extraction time of 32.4 min, and a liquid-to-solid ratio of 10.34:1. Under these conditions, PPI had high protein yield (26.1%) and functional properties (55.2% WSI, 3.6% WAC, 1.76 g/g OHC, 60.25 m2/g EA, 63.2% FA). Differential scanning calorimetry revealed that PPI was slightly denatured at 75.16 °C, and the amount of heat required to induce this denaturation was 0.03 J/g. SDS-PAGE presented protein bands that ranged from 14.4 to 66.2 kDa. Based on its functional properties, PPI can be used in the food industry.
DOI: 10.6084/m9.figshare.6045023.v1
title: Optimizing functional properties of perilla protein isolate using the response surface methodology

description: Abstract Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters of perilla protein isolate (PPI), which has functional properties. A central composite experimental design was applied to investigate the effects of three factors (ultrasonic power, extraction time, and liquid-to-solid ratio) on six responses: protein yield, water solubility index (WSI), water absorption capacity (WAC), oil holding capacity (OHC), emulsifying activity (EA), and foaming ability (FA). Based on the RSM results, the optimum conditions were an ultrasonic power of 206 W, and extraction time of 32.4 min, and a liquid-to-solid ratio of 10.34:1. Under these conditions, PPI had high protein yield (26.1%) and functional properties (55.2% WSI, 3.6% WAC, 1.76 g/g OHC, 60.25 m2/g EA, 63.2% FA). Differential scanning calorimetry revealed that PPI was slightly denatured at 75.16 °C, and the amount of heat required to induce this denaturation was 0.03 J/g. SDS-PAGE presented protein bands that ranged from 14.4 to 66.2 kDa. Based on its functional properties, PPI can be used in the food industry.
DOI: 10.6084/m9.figshare.6045020
title: Red wine produced from the Isabella and Ives cultivar (Vitis Labrusca): profile of volatiles and aroma descriptors

description: Abstract Considering the potential consumption and economic the importance that Isabella and Ives wines represent in the Brazilian consumer market as well as the scarcity of scientific data examining their quality, the objective of this study was to investigate the sensory quality and the volatiles profile of these wines. The volatile compounds were extracted by headspace solid-phase microextraction (HS-SPME) and a total of 54 compounds were detected in red wine samples including esters (23), terpenes (12), alcohols (10), aldehydes and ketones (5) and amines (1) as well as 3 compounds belonging to other classes. Isabella and Ives red wines were sensorially characterized by 14 descriptors, through quantitative descriptive analysis (QDA). The PCAs fruity descriptors were the primary contributors to the aroma profile of the analyzed wines due to the presence of ethyl acetate and esters, especially in the wine coded as QM, which exhibited the highest variety of compounds. The differences observed in the principal components analysis, might have been influenced by the grape composition of each wine. Although the wines were from the same region, each came from a different winery and was subject to unique production processes.
DOI: 10.6084/m9.figshare.6045020.v1
title: Red wine produced from the Isabella and Ives cultivar (Vitis Labrusca): profile of volatiles and aroma descriptors

description: Abstract Considering the potential consumption and economic the importance that Isabella and Ives wines represent in the Brazilian consumer market as well as the scarcity of scientific data examining their quality, the objective of this study was to investigate the sensory quality and the volatiles profile of these wines. The volatile compounds were extracted by headspace solid-phase microextraction (HS-SPME) and a total of 54 compounds were detected in red wine samples including esters (23), terpenes (12), alcohols (10), aldehydes and ketones (5) and amines (1) as well as 3 compounds belonging to other classes. Isabella and Ives red wines were sensorially characterized by 14 descriptors, through quantitative descriptive analysis (QDA). The PCAs fruity descriptors were the primary contributors to the aroma profile of the analyzed wines due to the presence of ethyl acetate and esters, especially in the wine coded as QM, which exhibited the highest variety of compounds. The differences observed in the principal components analysis, might have been influenced by the grape composition of each wine. Although the wines were from the same region, each came from a different winery and was subject to unique production processes.
DOI: 10.6084/m9.figshare.6045017
title: Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages

description: Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted protection in C57BL/6 mice by reducing the bacterial, which was correlated with increased serum concentration of IgG antibodies (IgG1 and IgG2a) against S. aureus. The increase in IgG2a antibody levels was correlated with a decrease of bacterial load in intradermally immunized C57BL/6 mice, along with production of IL-17A at the inflammation site, as well as IgG1consumption. Similar results were not found in the A/j lineage. In conclusion, a vaccine against S. aureus should focus more on the individual characteristics of the host because it is a determinant factor for the success of the immunization.
DOI: 10.6084/m9.figshare.6045017.v1
title: Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages

description: Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted protection in C57BL/6 mice by reducing the bacterial, which was correlated with increased serum concentration of IgG antibodies (IgG1 and IgG2a) against S. aureus. The increase in IgG2a antibody levels was correlated with a decrease of bacterial load in intradermally immunized C57BL/6 mice, along with production of IL-17A at the inflammation site, as well as IgG1consumption. Similar results were not found in the A/j lineage. In conclusion, a vaccine against S. aureus should focus more on the individual characteristics of the host because it is a determinant factor for the success of the immunization.
DOI: 10.6084/m9.figshare.6045011
title: Silage quality of six sorghum cultivars for sheep

description: Abstract: The objective of this work was to evaluate the silage quality, fermentation profile, intake, and digestibility of six sorghum cultivars for sheep feeding. 'SF 15', 'IPA 2502', 'BRS 655', 'BR 601', 'BRS 506', and 'Sudão' were cultivated in randomized complete block designs with five replicates, harvested, and ensiled when plants reached the soft-dough grain stage. Silage quality was determined in laboratory silos. Intake and apparent digestibility were determined during 22 days, using 24 Santa Inês lambs (35.5±2.21 kg) randomly distributed in the treatments. Silage from 'IPA 2502' had the lowest contents of dry matter, neutral detergent fiber, and acid detergent fiber; the highest nonfibrous carbohydrate and total digestible nutrient contents; and the greatest dry matter degradability. Silages from 'BR 601' and 'BRS 506' showed the highest values of crude protein, whereas those of 'SF 15' and 'Sudão' had the highest levels of fibrous fractions. Lambs fed silage from 'IPA 2502' consumed more dry matter and nonfibrous carbohydrates, and their crude protein digestibility was also greater than that from the consumption of other cultivar silages. 'IPA 2502' stands out for its nutritional characteristics, which resulted in better animal performance.
DOI: 10.6084/m9.figshare.6045011.v1
title: Silage quality of six sorghum cultivars for sheep

description: Abstract: The objective of this work was to evaluate the silage quality, fermentation profile, intake, and digestibility of six sorghum cultivars for sheep feeding. 'SF 15', 'IPA 2502', 'BRS 655', 'BR 601', 'BRS 506', and 'Sudão' were cultivated in randomized complete block designs with five replicates, harvested, and ensiled when plants reached the soft-dough grain stage. Silage quality was determined in laboratory silos. Intake and apparent digestibility were determined during 22 days, using 24 Santa Inês lambs (35.5±2.21 kg) randomly distributed in the treatments. Silage from 'IPA 2502' had the lowest contents of dry matter, neutral detergent fiber, and acid detergent fiber; the highest nonfibrous carbohydrate and total digestible nutrient contents; and the greatest dry matter degradability. Silages from 'BR 601' and 'BRS 506' showed the highest values of crude protein, whereas those of 'SF 15' and 'Sudão' had the highest levels of fibrous fractions. Lambs fed silage from 'IPA 2502' consumed more dry matter and nonfibrous carbohydrates, and their crude protein digestibility was also greater than that from the consumption of other cultivar silages. 'IPA 2502' stands out for its nutritional characteristics, which resulted in better animal performance.
DOI: 10.6084/m9.figshare.6045008
title: Performance during a strenuous swimming session is associated with high blood lactate: pyruvate ratio and hypoglycemia in fasted rats

description: The aim of this study was to investigate the effect of lactatemia elevation and glycemia reduction on strenuous swimming performance in fasted rats. Three rats were placed in a swimming tank at the same time. The first rat was removed immediately (control group) and the remaining ones were submitted to a strenuous swimming session. After the second rat was exhausted (Exh group), the third one was immediately removed from the water (Exe group). According to the period of time required for exhaustion, the rats were divided into four groups: low performance (3–7 min), low-intermediary performance (8–12 min), high-intermediary performance (13–17 min), and high performance (18–22 min). All rats were removed from the swimming tanks and immediately killed by decapitation for blood collection or anesthetized for liver perfusion experiments. Blood glucose, lactate, and pyruvate concentrations, blood lactate/pyruvate ratio, and liver lactate uptake and its conversion to glucose were evaluated. Exhaustion in low and low-intermediary performance were better associated with higher lactate/pyruvate ratio. On the other hand, exhaustion in high-intermediary and high performance was better associated with hypoglycemia. Lactate uptake and glucose production from lactate in livers from the Exe and Exh groups were maintained. We concluded that there is a time sequence in the participation of lactate/pyruvate ratio and hypoglycemia in performance during an acute strenuous swimming section in fasted rats. The liver had an important participation in preventing hyperlactatemia and hypoglycemia during swimming through lactate uptake and its conversion to glucose.
DOI: 10.6084/m9.figshare.6045008.v1
title: Performance during a strenuous swimming session is associated with high blood lactate: pyruvate ratio and hypoglycemia in fasted rats

description: The aim of this study was to investigate the effect of lactatemia elevation and glycemia reduction on strenuous swimming performance in fasted rats. Three rats were placed in a swimming tank at the same time. The first rat was removed immediately (control group) and the remaining ones were submitted to a strenuous swimming session. After the second rat was exhausted (Exh group), the third one was immediately removed from the water (Exe group). According to the period of time required for exhaustion, the rats were divided into four groups: low performance (3–7 min), low-intermediary performance (8–12 min), high-intermediary performance (13–17 min), and high performance (18–22 min). All rats were removed from the swimming tanks and immediately killed by decapitation for blood collection or anesthetized for liver perfusion experiments. Blood glucose, lactate, and pyruvate concentrations, blood lactate/pyruvate ratio, and liver lactate uptake and its conversion to glucose were evaluated. Exhaustion in low and low-intermediary performance were better associated with higher lactate/pyruvate ratio. On the other hand, exhaustion in high-intermediary and high performance was better associated with hypoglycemia. Lactate uptake and glucose production from lactate in livers from the Exe and Exh groups were maintained. We concluded that there is a time sequence in the participation of lactate/pyruvate ratio and hypoglycemia in performance during an acute strenuous swimming section in fasted rats. The liver had an important participation in preventing hyperlactatemia and hypoglycemia during swimming through lactate uptake and its conversion to glucose.