DOI: 10.5061/dryad.5hqbzkhfx
title: Data from: Postmating prezygotic isolation occurs at two levels of divergence in Drosophila recens and D. subquinaria

description: Identifying the presence and strength of reproductive isolating barriers
 is necessary to understand how species form and then remain distinct in
 the face of ongoing gene flow. Here we study reproductive isolation at two
 stages of the speciation process in the closely related mushroom-feeding
 species Drosophila recens and D. subquinaria. We assess three isolating
 barriers that occur after mating, including the number of eggs laid, the
 proportion of eggs laid that hatched, and the number of adult offspring
 from a single mating. First, all three reproductive barriers are present
 between D. recensfemales and D. subquinaria males, which are at the late
 stages of speciation but still produce fertile daughters through which
 gene flow can occur. There is no evidence for geographic variation in any
 of these traits, concurrent with patterns of behavioral isolation. Second,
 all three of these reproductive barriers are strong between geographically
 distant conspecific populations of D. subquinaria, which are in the early
 stages of speciation and show genetic differentiation and asymmetric
 behavioral discrimination. The reduction in the number of eggs laid is
 asymmetric, consistent with patterns in behavioral isolation, and suggests
 the evolution of postmating prezygotic isolation due to cascade
 reinforcement against mating with D. recens. In summary, not only may
 postmating prezygotic reproductive barriers help maintain isolation
 between D. recens and D. subquinaria but they may also drive the earliest
 stages of isolation within D. subquinaria. 
description: The data are the raw behavioral data and counts of eggs, their
 hatch rate, and number of adult offspring produced.  
description: # Data from: Postmating prezygotic isolation occurs at two levels of
 divergence in Drosophila recens and D. subquinaria
 [https://doi.org/10.5061/dryad.5hqbzkhfx](https://doi.org/10.5061/dryad.5hqbzkhfx) Authors: Duitsman, Bomar, Powell, Dyer. Journal of Evolutionary Biology ## Description of the data and file structure Data include 1) behavioral assays of mating rate, copulation latency, and copulation duration; 2) number of eggs laid in 6 days, 3) proportion of eggs laid that hatched, 3) number of adult offspring produced after 20 days of egg laying.  Data are included for crosses to test for 1) reproductive isolation between Drosophila recens females and D. subquinaria males and 2) between geographically distinct D. subquinaria populations.  ### Files and variables: **JEB-2023-00304_matings.csv** Experiment = Recens x Subquinaria or within subquinaria Female = female species and population Male = male species and population Cross_FxM = Crosses are listed female x male N_crosses = number of crosses set up N_matings = number of crosses set up that mated **JEB-2023-00304_SxR.csv** Experiment: either fitness (offspring scored) or eggs produced/hatched Fly_ID_dyrad: individual fly ID Cross_FxM: Cross of female x male, noted as R_NY = recens NY, R_AB = recens alberta, S_MT = subquinaria montana, Block: block Copn Latency: copulation latency, time from male inserted to copulation started, noted in seconds. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.  Copn Duration: copulation duration, time from copulation start to end, noted in seconds. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.  Dead = the female died during the 6 or 20 days of the experiment or excaped during a transfer; number of offspring or eggs were not included. died = fly died, no = fly lived for the duration of the experiment.  Total offspring: number of offspring produced by the female across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment. total eggs hatched: total number of eggs hatched of those that were laid across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment. total eggs laid: total eggs laid across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment. Hatch day #, etc: number of eggs hatched in # egg laying vial. null = no data, either not relevant to this cross or fly died. na= no data because not relevant to the experiment. Total day #, : number of eggs laid in # egg laying vial. null = no data usually because the fly died. na = no data because not relevant to this experiment. **JEB-2023-00304_SxS.csv** Experiment: either from the fitness experiment (offspring counted) or egg laying experiment (eggs scored) Fly_ID_dyrad: individual fly ID Cross_FxM: Cross of female x male, noted as OR = subquinaria oregon, AB = subquinaria alberta Block: block Copn Latency: copulation latency, time from male inserted to copulation started, noted in seconds. na = we did not record the time the male was placed in the vial or the beginning or end of copulation. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.  Copn Duration: copulation duration, time from copulation start to end, noted in seconds. na = we did not record the time the male was placed in the vial or the beginning or end of copulation. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.  Dead = the female died during the 6 or 20 days of the experiment or excaped during a transfer; number of offspring or eggs were not included. died = fly died, no = fly lived for the duration of the experiment.  Total offspring: number of offspring produced by the female across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment. total eggs hatched: total number of eggs hatched of those that were laid across all days total eggs laid: total eggs laid across all days Hatch day #, etc: number of eggs hatched in # egg laying vial Total day #, : number of eggs laid in # egg laying vial