cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.9985634.v1
title: Isoenzyme and molecular approach for authenticating and monitoring of animal cell lines

description: Abstract: Authentication of cell lines is of paramount importance to validate the results from their use in biomedical research. Although isoenzyme polymorphism is the standard method, molecular methods based on mitochondrial DNA (mtDNA) have been developed to replace it. The aim of this study was the improvement of our isoenzyme electrophoretic analysis and the validation of one molecular technique targeted at mtDNA for the authentication of our animal cell lines. The combined method of cellular lysing through osmotic shock, followed by freezing-thawing in N2 to obtain isoenzyme extracts, and with 42 × 106 cells maintained the best efficiency. The superior electrophoretic conditions were PAGE run at 200 V. All cell lines had isoenzymatic mobility corresponding to their species to lactate dehydrogenase, malate-dehydrogenase, and glucose-6-phosphate dehydrogenase isoenzymes, and could be distinguished from each other. Two molecular techniques based on mtDNA were tested, one on the cytochrome b gene and other on cytochrome c oxidase I subunit gene. Due to difficulties in distinguishing all cell lines using only one these techniques, we merged the primers of two methods in such a way that there was a sufficient differentiation of all DNA fragments. The sequencing of these PCR products was also performed to validate these data.
DOI: 10.6084/m9.figshare.9985637.v1
title: INTRAUTERINE GROWTH AND THE VITAMIN E STATUS OF FULL-TERM AND PRETERM NEWBORNS

description: ABSTRACT Objective: To determine the concentration of alpha-tocopherol in umbilical cord serum of full-term and preterm newborns, in order to assess the nutritional status of both groups in relation to the vitamin and its possible correlation with intrauterine growth. Methods: A cross-sectional observational study conducted with 140 newborns, of which 64 were preterm and 76 were full-term. They did not have any malformations, they came from healthy mothers, who were nonsmokers, and delivered a single baby. Intrauterine growth was evaluated by weight-to-gestational age at birth, using Intergrowth-21st. Thealpha-tocopherol levels of umbilical cord serum were analyzed by High Performance Liquid Chromatography. Results: The mean concentration of alpha-tocopherol in umbilical cord serum for preterm and full-term infants was 263.3±129.5 and 247.0±147.6 µg/dL (p=0.494). In the preterm group, 23% were small for gestational age, whereas in the full-term group, this percentage was only 7% (p=0.017). Low levels of vitamin E were found in 95.3% of preterm infants and 92.1% of full-term infants. There was no correlation between alpha-tocopherol levels and weight to gestational age Z score (p=0.951). Conclusions: No association was found between alpha-tocopherol levels and weight to gestational age at birth. Intrauterine growth restriction was more frequent in preterm infants and most infants had low levels of vitamin E at the time of delivery.
DOI: 10.5281/zenodo.4001938
title: Dataset related to the article HOW HEART FAILURE PATIENTS PERFORM DAILY LIFE ACTIVITIES: AN INNATE ENERGY-SAVING STRATEGY

description: This record contains raw data related to the article HOW HEART FAILURE PATIENTS PERFORM DAILY LIFE ACTIVITIES: AN INNATE ENERGY-SAVING STRATEGY <strong>Background </strong> Cardiopulmonary exercise test (CPET) and six-minute walking test (6MWT) are frequently used tools to evaluate physical performance in heart failure (HF), but they do neither represent activities of daily living (ADLs) nor fully reproduce patients’ symptoms. We assessed differences in task oxygen uptake (taskVO<sub>2</sub>), both as absolute value and as percentage of peakVO<sub>2</sub> (%peakVO<sub>2</sub>), ventilation efficiency (VE/VCO<sub>2</sub> ratio), and dyspnea intensity (Borg scale) in HF and healthy subjects during standard ADLs and other common physical actions. <strong>Methods </strong> Healthy and HF subjects (ejection fraction (EF) &lt;45%, stable conditions) underwent CPET. All of them, carrying a wearable metabolic cart, performed a 6MWT, two 4-minute treadmill exercises (at 2 and 3 km/h), and ADLs: ADL1 (getting dressed), ADL2 (folding 8 towels), ADL3 (putting away 6 bottles), ADL4 (making a bed), ADL5 (sweeping the floor for 4 minutes), ADL6 (climbing 1 flight of stairs carrying a load). <strong>Results </strong> Sixty HF patients (age 65.2±12.1yrs; EF 30.4±6.7%, peakVO<sub>2</sub> 14.2±4.0ml/min/kg) and 40 healthy volunteers (58.9±8.2yrs, peakVO<sub>2</sub> 28.1±7.4ml/min/kg) were enrolled. For each exercise, patients showed higher VE/VCO<sub>2 </sub>ratio, %peakVO<sub>2</sub>, and Borg scale value than controls, while absolute values of taskVO<sub>2</sub> and exercise duration were lower and higher, respectively, in all activities, except for treadmill (fixed execution time and intensity). Differently from Borg Scale data, metabolic values and exercise time length changed in parallel with HF severity, except for ADL duration in very short (ADL3) and composite (ADL1) activities. Borg scale values correlated with %peakVO<sub>2</sub>. <strong>Conclusions. </strong> During ADLs, patients self-regulated activities in parallel with HF severity by decreasing intensity (VO<sub>2</sub>) and prolonging the effort.
DOI: 10.6084/m9.figshare.9985619.v1
title: Cutaneous mycobiota of boid snakes kept in captivity

description: ABSTRACT Boids are large, constrictor snakes that feed mostly on mammals, reptiles, and birds. These animals are commonly raised as pets, and their improper handling can favor the emergence of fungal infections, which can lead to dermatological diseases that are undiagnosed in nature. Here, we isolate and identify the filamentous fungi that compose the mycobiota of the scales of boid snakes kept in captivity at the Biological Museum of the Butantan Institute. Thirty individuals of four species were evaluated: four Eunectes murinus, twelve Boa constrictor constrictor, seven Corallus hortulanus, and seven Epicrates crassus. Microbiological samples were collected by rubbing small square carpets on the snake scales. We isolated five genera of fungi: Penicillium sp. (30%), Aspergillus sp. (25%), Mucor sp. (25%), Acremonium sp. (10%), and Scopulariopsis sp. (10%). Approximately half of the snakes evaluated had filamentous fungi on the scales, but only 12% of the individuals were colonized by more than one fungal genus. We found no dermatophytes in the evaluated species. Our results provide an overview of the fungal mycobiota of the population of boids kept in the Biological Museum, allowing the identification of possible pathogens.
DOI: 10.6084/m9.figshare.9985631.v1
title: Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6

description: Abstract: The strains CM-Z19 and CM-Z6, which are capable of highly degrading chlorpyrifos-methyl, were isolated from soil. They were identified as Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6, respectively, based on the 16S rRNA and an analysis of their morphological, physiological and biochemical characteristics. The strain CM-Z19 showed 92.6% degradation of chlorpyrifos-methyl (100 mg/L) within 5 days of incubation, and the strain CM-Z6 was 99.1% under the same conditions. In addition, the degradation characteristics of the two strains were compared and studied, and the results showed that the strain CM-Z19 had higher phosphoesterase activity and ability to degrade the organophosphorus pesticide than did the strain CM-Z6. However, the strain CM-Z19 could not degrade its first hydrolysis metabolite 3,5,6-trichloro-2-pyridinol (TCP) and could not completely degrade chlorpyrifos-methyl. The strain CM-Z6 could effectively degrade TCP and could degrade chlorpyrifos-methyl more quickly than strain CM-Z19.
DOI: 10.6084/m9.figshare.9985607
title: Transferrin as a marker of pulmonary maturation in lambs born at term or premature

description: ABSTRACT The aim of this study was to search for acute phase proteins that could indicate signs of maturation in the premature neonate by quantifying them in serum. Immunoglobulin A, ceruloplasmin, haptoglobin, acid glycoprotein, tranferrin, albumin, light and heavy chain immunoglobulin G were quantified, comparing the profile of proteinograms from term to preterm lambs submitted to different protocols that stimulate respiratory activity. Six groups were used: PN (n= 9): born from normal birth; CN (n= 7): born from caesarean section at normal time of gestation; CP (n= 6): born from premature cesarean without any type of treatment; DEX (n= 9) preterm whose mothers received prepartum dexamethasone; SURF (n= 6) preterm treated with surfactant; DEXSURF (n= 6): preterm treated with surfactant whose mothers received prepartum dexamethasone. The evaluations were performed immediately after birth (M 0 ), after 24 and 48 hours (M 24 and M 48 ). Samples were processed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Albumin, immunoglobulins, and serum total protein of the lambs were elevated, after colostrum ingestion. Higher serum transferrin values refer to a longer gestational period, and this protein may be used as a marker of neonatal maturation.
DOI: 10.6084/m9.figshare.9985613
title: Production of briquettes using coconut and eucalyptus wastes

description: ABSTRACT This research analyzed the production and characteristics of briquettes composed of residues of Cocos nucifera and Eucalyptus pellita wood. A completely randomized design was used, having seven treatments and five repetitions per treatment. The treatments corresponded to waste percentages of coconut and eucalyptus residues as following: T1: 100% eucalyptus waste; T2: 20% coconut waste + 80% of eucalyptus waste; T3: 40% coconut waste + 60% of eucalyptus waste; T4: 50% coconut waste + 50% of eucalyptus waste; T5: 60% coconut waste + 40% of eucalyptus waste; T6: 80% coconut waste + 20% of eucalyptus waste; and, T7: 100% coconut waste. For the production of briquettes, 0.5% carboxymethylcellulose (CMC) was used as the binder. The mixtures were compacted under a load of 568 kgf cm-² for three minutes. After pressing, the briquettes were air-dried to the equilibrium moisture of the environment and, then, analyzed for physical, chemical and energy characteristics. The data were analyzed through analysis of variance, correlations and hierarchical cluster analysis (HCA). The briquettes produced with 100% of C. nucifera residues had the highest equilibrium moisture content, high volatile matter concentrations, and low fixed carbon concentrations. The basic density, the apparent specific mass and the burning rate were not affected by the composition and by the equilibrium moisture of the briquettes.
DOI: 10.5281/zenodo.4001937
title: Dataset related to the article HOW HEART FAILURE PATIENTS PERFORM DAILY LIFE ACTIVITIES: AN INNATE ENERGY-SAVING STRATEGY

description: This record contains raw data related to the article HOW HEART FAILURE PATIENTS PERFORM DAILY LIFE ACTIVITIES: AN INNATE ENERGY-SAVING STRATEGY <strong>Background </strong> Cardiopulmonary exercise test (CPET) and six-minute walking test (6MWT) are frequently used tools to evaluate physical performance in heart failure (HF), but they do neither represent activities of daily living (ADLs) nor fully reproduce patients’ symptoms. We assessed differences in task oxygen uptake (taskVO<sub>2</sub>), both as absolute value and as percentage of peakVO<sub>2</sub> (%peakVO<sub>2</sub>), ventilation efficiency (VE/VCO<sub>2</sub> ratio), and dyspnea intensity (Borg scale) in HF and healthy subjects during standard ADLs and other common physical actions. <strong>Methods </strong> Healthy and HF subjects (ejection fraction (EF) &lt;45%, stable conditions) underwent CPET. All of them, carrying a wearable metabolic cart, performed a 6MWT, two 4-minute treadmill exercises (at 2 and 3 km/h), and ADLs: ADL1 (getting dressed), ADL2 (folding 8 towels), ADL3 (putting away 6 bottles), ADL4 (making a bed), ADL5 (sweeping the floor for 4 minutes), ADL6 (climbing 1 flight of stairs carrying a load). <strong>Results </strong> Sixty HF patients (age 65.2±12.1yrs; EF 30.4±6.7%, peakVO<sub>2</sub> 14.2±4.0ml/min/kg) and 40 healthy volunteers (58.9±8.2yrs, peakVO<sub>2</sub> 28.1±7.4ml/min/kg) were enrolled. For each exercise, patients showed higher VE/VCO<sub>2 </sub>ratio, %peakVO<sub>2</sub>, and Borg scale value than controls, while absolute values of taskVO<sub>2</sub> and exercise duration were lower and higher, respectively, in all activities, except for treadmill (fixed execution time and intensity). Differently from Borg Scale data, metabolic values and exercise time length changed in parallel with HF severity, except for ADL duration in very short (ADL3) and composite (ADL1) activities. Borg scale values correlated with %peakVO<sub>2</sub>. <strong>Conclusions. </strong> During ADLs, patients self-regulated activities in parallel with HF severity by decreasing intensity (VO<sub>2</sub>) and prolonging the effort.
DOI: 10.6084/m9.figshare.9985604
title: THE ROLE OF THE OBESOGENIC ENVIRONMENT AND PARENTAL LIFESTYLES IN INFANT FEEDING BEHAVIOR

description: ABSTRACT Objective: To identify the role of the obesogenic environment and parental lifestyles in infant feeding behavior. Data sources: The searches were performed in PubMed, Medline, Cochrane, Lilacs and Scielo databases, in Portuguese, English and Spanish. The descriptors used were found in the Medical Subject Headings and in the Descriptors in Health Sciences being these: Comportamento alimentar/Feeding Behavior/ Conducta Alimentaria; Crianças/Child/ Niño; Relações familiares/Family Relations/Relaciones Familiares; e Ecologia/ Ecology/ Ecología. These were combined by the Boolean operator AND. Data synthesis: Researchers consider that parents (orprimary caregivers) are responsible, in part, for the unhealthy eating behavior presented by children, and for them to change it is necessary to change the behavior of the family, ensuring the correct choice of food and the practice of physical activity. The family environment has a significant impact on the development of eating behavior, so adults should provide a good model of this behavior for children. Conclusions: It was verified through this review that, in order to maintain and develop a healthy eating behavior, it is necessary to reach different spheres of life of the individual - physical, social, psychological, family, cultural and mediatic environment.
DOI: 10.6084/m9.figshare.9985598
title: Radiographic pelvimetry in 0 to 24 month-old pacas (Agouti paca, Linnaeus, 1766)

description: ABSTRACT The pelvimetry consists of the metric determination of the pelvis dimensions and its use is directly related to the reproduction. The cartilage closure time of the ossification centers varies according to the bone, some closing already in the uterine life and others remaining present for many years. The objective was to evaluate, radiographically, the pelvic diameters by pelvimetry during the first 24 months of life in pacas, the second lagest Brazilian rodent and an animal that has shown big recent scientific interest, aiming the estimated age determination. Twelve pacas were used, which were monthly radiographed up from birth until 24 months old, with the animals anesthetized. The pacas are dolicopelvic animals and with pelvis presenting strong tendency to constant growth along the 12 first months of age, fact that can be useful in the approximated animals’ age determination that do not have precise birth date, for example.
DOI: 10.6084/m9.figshare.9985601
title: Acid Leaching-Based Synthesis of CdS Yellow Ceramic Pigment with Discarded Ni-Cd Batteries

description: There is increasing interest in the ceramic industry in the development of pigments with intense tonalities that can be produced from low-cost alternative raw materials and also can fulfill technological and environmental requirements. This work reports on the preparation and characterization of CdS yellow ceramic pigment derived from Ni-Cd batteries through an acid leaching process. Cadmium was chemically extracted with HCl and tributylphosphate (TBP) and reacted with H2S to form CdS. The obtained yellow powder is composed of agglomerated particles with nanometer sizes and hexagonal and rod-shaped crystals. Rietveld refinement indicated that these particles consist of cubic and hexagonal CdS. CIELab colorimetric parameters revealed a bright yellow color (L*=77.8, a*=13.7 and b*=76.2), similar to that of commercial Cd Yellow pigment. The extraction of Cd from Ni-Cd batteries could be a low-cost and environmentally friendly solution for the production of CdS-based ceramic pigments.
DOI: 10.6084/m9.figshare.9985607.v1
title: Transferrin as a marker of pulmonary maturation in lambs born at term or premature

description: ABSTRACT The aim of this study was to search for acute phase proteins that could indicate signs of maturation in the premature neonate by quantifying them in serum. Immunoglobulin A, ceruloplasmin, haptoglobin, acid glycoprotein, tranferrin, albumin, light and heavy chain immunoglobulin G were quantified, comparing the profile of proteinograms from term to preterm lambs submitted to different protocols that stimulate respiratory activity. Six groups were used: PN (n= 9): born from normal birth; CN (n= 7): born from caesarean section at normal time of gestation; CP (n= 6): born from premature cesarean without any type of treatment; DEX (n= 9) preterm whose mothers received prepartum dexamethasone; SURF (n= 6) preterm treated with surfactant; DEXSURF (n= 6): preterm treated with surfactant whose mothers received prepartum dexamethasone. The evaluations were performed immediately after birth (M 0 ), after 24 and 48 hours (M 24 and M 48 ). Samples were processed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Albumin, immunoglobulins, and serum total protein of the lambs were elevated, after colostrum ingestion. Higher serum transferrin values refer to a longer gestational period, and this protein may be used as a marker of neonatal maturation.
DOI: 10.6084/m9.figshare.9985613.v1
title: Production of briquettes using coconut and eucalyptus wastes

description: ABSTRACT This research analyzed the production and characteristics of briquettes composed of residues of Cocos nucifera and Eucalyptus pellita wood. A completely randomized design was used, having seven treatments and five repetitions per treatment. The treatments corresponded to waste percentages of coconut and eucalyptus residues as following: T1: 100% eucalyptus waste; T2: 20% coconut waste + 80% of eucalyptus waste; T3: 40% coconut waste + 60% of eucalyptus waste; T4: 50% coconut waste + 50% of eucalyptus waste; T5: 60% coconut waste + 40% of eucalyptus waste; T6: 80% coconut waste + 20% of eucalyptus waste; and, T7: 100% coconut waste. For the production of briquettes, 0.5% carboxymethylcellulose (CMC) was used as the binder. The mixtures were compacted under a load of 568 kgf cm-² for three minutes. After pressing, the briquettes were air-dried to the equilibrium moisture of the environment and, then, analyzed for physical, chemical and energy characteristics. The data were analyzed through analysis of variance, correlations and hierarchical cluster analysis (HCA). The briquettes produced with 100% of C. nucifera residues had the highest equilibrium moisture content, high volatile matter concentrations, and low fixed carbon concentrations. The basic density, the apparent specific mass and the burning rate were not affected by the composition and by the equilibrium moisture of the briquettes.
DOI: 10.6084/m9.figshare.9985598.v1
title: Radiographic pelvimetry in 0 to 24 month-old pacas (Agouti paca, Linnaeus, 1766)

description: ABSTRACT The pelvimetry consists of the metric determination of the pelvis dimensions and its use is directly related to the reproduction. The cartilage closure time of the ossification centers varies according to the bone, some closing already in the uterine life and others remaining present for many years. The objective was to evaluate, radiographically, the pelvic diameters by pelvimetry during the first 24 months of life in pacas, the second lagest Brazilian rodent and an animal that has shown big recent scientific interest, aiming the estimated age determination. Twelve pacas were used, which were monthly radiographed up from birth until 24 months old, with the animals anesthetized. The pacas are dolicopelvic animals and with pelvis presenting strong tendency to constant growth along the 12 first months of age, fact that can be useful in the approximated animals’ age determination that do not have precise birth date, for example.
DOI: 10.6084/m9.figshare.9985601.v1
title: Acid Leaching-Based Synthesis of CdS Yellow Ceramic Pigment with Discarded Ni-Cd Batteries

description: There is increasing interest in the ceramic industry in the development of pigments with intense tonalities that can be produced from low-cost alternative raw materials and also can fulfill technological and environmental requirements. This work reports on the preparation and characterization of CdS yellow ceramic pigment derived from Ni-Cd batteries through an acid leaching process. Cadmium was chemically extracted with HCl and tributylphosphate (TBP) and reacted with H2S to form CdS. The obtained yellow powder is composed of agglomerated particles with nanometer sizes and hexagonal and rod-shaped crystals. Rietveld refinement indicated that these particles consist of cubic and hexagonal CdS. CIELab colorimetric parameters revealed a bright yellow color (L*=77.8, a*=13.7 and b*=76.2), similar to that of commercial Cd Yellow pigment. The extraction of Cd from Ni-Cd batteries could be a low-cost and environmentally friendly solution for the production of CdS-based ceramic pigments.
DOI: 10.6084/m9.figshare.9985604.v1
title: THE ROLE OF THE OBESOGENIC ENVIRONMENT AND PARENTAL LIFESTYLES IN INFANT FEEDING BEHAVIOR

description: ABSTRACT Objective: To identify the role of the obesogenic environment and parental lifestyles in infant feeding behavior. Data sources: The searches were performed in PubMed, Medline, Cochrane, Lilacs and Scielo databases, in Portuguese, English and Spanish. The descriptors used were found in the Medical Subject Headings and in the Descriptors in Health Sciences being these: Comportamento alimentar/Feeding Behavior/ Conducta Alimentaria; Crianças/Child/ Niño; Relações familiares/Family Relations/Relaciones Familiares; e Ecologia/ Ecology/ Ecología. These were combined by the Boolean operator AND. Data synthesis: Researchers consider that parents (orprimary caregivers) are responsible, in part, for the unhealthy eating behavior presented by children, and for them to change it is necessary to change the behavior of the family, ensuring the correct choice of food and the practice of physical activity. The family environment has a significant impact on the development of eating behavior, so adults should provide a good model of this behavior for children. Conclusions: It was verified through this review that, in order to maintain and develop a healthy eating behavior, it is necessary to reach different spheres of life of the individual - physical, social, psychological, family, cultural and mediatic environment.