DOI: 10.5061/dryad.np5hqc054
title: Data from: Asymmetric micro-evolutionary responses in a warming world: Heat-driven adaptation enhances metal tolerance, but not vice versa

description: We investigated how prior adaptation to either high temperature or copper
 (Cu) contamination influences subsequent tolerance to the other stressor
 in populations of the freshwater zooplanktonic rotifer Brachionus
 calyciflorus (Pallas 1766). Using an experimental evolution approach, we
 subjected populations to either gradually increasing Cu levels, elevated
 temperature, or control conditions over multiple generations.
 Subsequently, we conducted a common garden experiment to assess the effect
 of selection history on population tolerance. We found that heat-adapted
 populations exhibited increased tolerance to Cu, whereas Cu-adapted
 populations showed no enhanced tolerance to high temperatures.
description: We conducted a selection experiment followed by a common garden
 experiment. In the selection experiment, we exposed 9 genetically
 identical populations of the freshwater monogonont rotifer Brachionus
 calyciflorus s.s.to three treatments, i.e. a benign control treatment, a
 copper addition treatment, and a high-temperature treatment. All
 populations underwent six cycles (Cycles 1 to 6); during each cycle clonal
 population growth was followed by sexual reproduction and the formation of
 dormant propagules. In the copper addition treatment, copper levels were
 stepwise increased at the beginning of each cycle (from 30, 45, 50, 55,
 57.5, 60 to 62.5 ug Cu/L in Cycles 1 to 6, respectively). In the
 temperature treatment, temperature levels were stepwise increased at the
 beginning of each cycle (from 24, 28, 30, 32, 35 and 35.5 °C,
 respectively). Dormant propagules produced during each cycle were
 stored. <br>For the common garden experiment, we used propagules
 produced at the end of Cycle 6 to established four clonal lines from each
 population. In addition, we used six ancestral clones (i.e. randomly
 selected from the set of clones that were used to initiate the selection
 experiment with). Populations of all clonal lines were subjected to a
 multifactorial combination of a Cu and Heat treatment, i.e. Control
 conditions (22 °C, no Cu addition), a Cu treatment (22 °C, 62.5 µg Cu/L),
 a heat treatment (34 °C, no Cu addition) and exposure to a combination of
 Cu and heat (34 °C, 62.5 µg Cu/L). After an acclimation phase, demographic
 variables of experimental populations were monitored for 5 days.
description: # Asymmetric micro-evolutionary responses in a warming world: heat-driven
 adaptation enhances metal tolerance, but not vice versa *
 "Han_et_al._Demographic_data_common_garden_experiment.csv"
 Grouping variables: Select_history: Treatment in the selection experiment
 (four levels: Ancestral, Control, Copper, Temp). Hatching: refers to
 different moments in time during which clonal lines were established from
 dormant propagules (three levels: H1: cultured during approximately 12
 months prior to common garden experiment; H2: cultured during 3 to 6
 months prior to CG experiment; Ancestral: in culture since hatching from
 pond sediments. Treat: common garden treatment (four levels: Control, Cu,
 Heat, Cu+Heat) Day: day in the 5-day common garden experiment (levels: 1
 to 5). Pop_Origin: Identity of experimental units in the evolution
 experiment (10 levels, i.e. 9 populations of selection experiment + 1
 Ancestral population). Clone: identity of clone (42 levels) PopID:
 Identity of individual populations in the common garden experiment (168
 levels) (Note that half of the clones of Control and Cu-selected
 populations were lost during the acclimation phase in the Cu+Heat
 treatment, ending up as NA in the datafile). Demographic variables
 (counts): FPE0: females with zero eggs FPE1: females with one
 parthenogenetic egg FPE2: females with two parthenogenetic eggs FPE3:
 females with three parthenogenetic eggs FUSE: mictic females with
 unfertilized sexual eggs FRE: females with resting egg FDEAD: dead
 rotifers LRE: loose resting eggs TotalF: total number of females at end of
 interval InitialF: total number of females at beginning of interval *
 "Han_et_al._Copper_data.csv" For methodological details: See
 "Cu determination experiment", Supplementary Information of
 "Asymmetric micro-evolutionary responses in a warming world:
 heat-driven adaptation enhances metal tolerance in a planktonic rotifer,
 but not vice versa" by Han et al. 2025, Global Change Biology
 **Grouping variables:** Treat: common garden treatment (three levels:
 Control, Cu, Cu+Heat) Replica: replicate culture (three per treatment)
 Cu_addition: theoretical end concentration of addition (µg/L)
 Cu_concentration: measured concentration (µg/L)