DOI: 10.5061/dryad.ms6b9
title: Data from: Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean

description: Porites white patch syndrome (PWPS) is a coral disease recently described
 in the western Indian Ocean. This study aimed to isolate and identify
 potential pathogens associated with PWPS utilising both culture and
 non-culture screening techniques and inoculation trials. A total of 14
 bacterial strains (those dominant in disease lesions, absent or rare in
 healthy tissues and considered potential pathogens in a previous study)
 were cultured and used to experimentally inoculate otherwise healthy
 individuals in an attempt to fulfil Henle-Koch's postulates. However,
 only one (P180R), identified as closely related (99-100% sequence identity
 based on 1.4 kb 16S RNA sequence) to Vibrio tubiashii, elicited signs of
 disease in tank experiments. Following experimental infection (which
 resulted in a 90% infection rate), the pathogen was also successfully
 re-isolated from the diseased tissues and re-inoculated in healthy corals
 colonies, therefore fulfilling the final stages of Henle-Koch's
 postulates. Finally we report that PWPS appears to be a
 temperature-dependent disease, with significantly higher tissue loss
 (ANOVA: df= 2, F=39.77, P&lt;0.01) occurring at 30°C (1.45 ± 0.85 cm2
 day−1 (mean ± SE)) compared to ambient temperatures of 28°C and 26°C (0.73
 ± 0.80 cm2 day−1 (mean ± SE) and 0.51 ± 0.50 cm2 day−1 (mean ± SE),
 respectively).
description: PWPS bacterial
 culturesSequences (16S rRNA
 gene) of the bacterial isolates used in phase 1 of the inoculation
 experiment. The software used to create the data file (fasta) is
 Genesious.Bacterial candidates
 (Infection trials)Sequences (16S rRNA
 gene) of the bacterial strains (potential PWPS pathogens) used for
 infectivity experiements. The software used to create the data file
 (Fasta) is GENEIOUS Pro (V.5.6.6).Nucleotide alignment
 (16S rRNA gene)Nucleotide alignment of
 the isolate "P180R" (16S rRNA gene) performed with reference
 sequences related to multiple bacterial strains selected from NCBI using
 GENEIOUS alignment method.MLST
 alignmentMutlilocus sequence
 typing (MLST) alignment of the stain P180R using the partial sequences of
 16S rRNA (1422 bp), pyrH (590 bp), recA (766 bp) and rpoA (802bp) genes.
 The sequences obtained for each gene were aligned with reference sequences
 related to multiple bacterial strains selected from NCBI using GENEIOUS
 alignment methodNucleotide alignment
 (pyrH gene)Nucleotide alignment of
 the isolate "P180R" (pyrH gene) performed with reference
 sequences related to multiple bacterial strains selected from NCBI using
 GENEIOUS alignment method.PWPS bacterial cultures
 (corresponding codes and blast results)Bacterial strains
 isolated from PWPS-infected Porites colonies.This is a "xlsx"
 file containing each bacterial strain codes with their respective blast
 results.Tree for the 16S rRNA
 geneNeighbour-joining
 phylogenetic tree for the 16S rRNA gene gene showing the relatedness of
 the strain P180R with reference Vibrio strains. The software used to
 create the data (Newick) is Geneious 6.1.5.Tree for the pyrH
 genePhylogenetic trees built
 by the neighbour-joining method using the NKY model of GENEIOUSTM Pro
 (V.6.1.5) with bootstrap values based on 1000 replicates for the isolate
 "P180R" (pyrH gene).Consensus tree
 (concatanation)Consensus tree built by
 the neighbour-joining method using the NKY model of GENEIOUS Pro (V.6.1.5)
 with bootstrap values based on 1000 replicates for the isolate
 "P180R" (Partial sequences of 16S rRNA (1422 bp), pyrH (590 bp),
 recA (766 bp) and rpoA (802bp)).