DOI: 10.5061/dryad.rr4xgxdkq
title: Data from: Diet-dependent induction of life-history changes in Daphnia: 5α-cyprinol sulfate is not the kairomone

description: Daphnia responds to chemical cues released by fish with adaptive changes
 in behavior (DVM), morphology, and life history traits, e. g. a reduction
 in size at first reproduction (SFR). For cyprinid fish, it has been
 revealed that DVM and changes in morphology of Daphnia are induced by the
 bile salt 5α-cyprinolsulfate (CPS), while the substance that induces
 life-history changes is unknown. Here we show that C18-solid phase
 extracts from fish incubation water of roach (Rutilus rutilus) that have
 fed Daphnia (RfD) induce a reduction of SFR in D. magna while similar
 extracts with chironomid larvae as prey do not. Subsequent fractionation
 of RfD by HPLC revealed that the major bile salts of cyprinid fish, CPS
 and taurochenodeoxycholic acid, eluted outside the active fractions, while
 taurocholic acid (TCA) had its peak within the active fractions. A
 dose-response experiment revealed that up to 2 µM TCA did not reduce SFR,
 which excludes TCA as a potential kairomone. In conclusion, we show that
 all major bile salts of cyprinid fish do not cause the observed reduction
 in SFR, which points to an unknown kairomone that proves to be negatively
 charged.
description: # Data from: Diet-dependent induction of life-history changes in Daphnia:
 5α-cyprinol sulfate is not the kairomone Dataset DOI:
 [10.5061/dryad.rr4xgxdkq](10.5061/dryad.rr4xgxdkq) ## Description of the
 data and file structure Diet-dependent induction of life-history changes
 in *Daphnia*: 5α-cyprinol sulfate is not the kairomone The Excel dataset
 'Diet-dependent induction of life-history changes in *Daphnia*:
 5α-cyprinol sulfate is not the kairomone' contains the full set of
 raw data. The individual experiments are separated by tabs and named after
 the figures. Abbreviations are listed within the Excel sheets. Also,
 unformatted CSV files for each figure are included for the analysis. ###
 Files and variables Life-history experiments **Figure 1:** Life-history
 data of the RfD interaction with a C18-cartridge. The table represents
 measurements of Size at First Reproduction (SFR)—expressed in
 millimeters—for different experimental treatments that interact with a C18
 cartridge. Each treatment was measured across three replicates, and the
 mean SFR for each replicate is reported. **Figure 2:** Data from the
 life-history experiments of RfD 'Roach fed with *Daphnia*'
 (1–3), as well as the raw data from the life-history experiments with RfL
 'Roach fed with larvae'. Notice: In all tables, 'n.d.'
 indicates that non-detectable values are present. **Figure 3:**
 Life-history data of the individual fractions (1–10) of the three
 different RfD fishwater extracts, and the results of quantifying the three
 different bile salts (CPS, TCA, and TCDCA) via LC-MS. **Figure 4:**
 Life-history raw data of the TCA threshold test. The table shows the Size
 at First Reproduction (SFR)—measured in millimeters—for organisms exposed
 to different concentrations of TCA (presumably a chemical or test
 compound). Each concentration was tested in three replicates, and the mean
 SFR for each replicate is listed. **Figure 5:** Life-history raw data of
 the RfD interaction with the strong anion exchanger. The table reports the
 Size at First Reproduction (SFR)—in millimeters—for different treatments
 interacting with a strong anion exchanger. Each treatment includes four
 replicates, with the mean SFR shown for each. **Fig. S2:** Life history
 raw data of RfD fractions after use of the strong anion exchanger. The
 table presents the Size at First Reproduction (SFR) in millimeters for
 different fractions obtained after passing Roach-fed-with-Daphnia (RfD)
 samples through a strong anion exchanger. Each fraction includes three
 replicates. ## Code/software There is no special software needed to view
 the data except MS Excel. ## Access information Other publicly accessible
 locations of the data: * None Data was derived from the following sources:
 * None