DOI: 10.22002/a8236-c6r42
title: Caulobacter crescentus

description: Yves Brun strain 2845: CB15-del-hfsD. 4 of 5, holdfast mutants from Yves Brun, had to convert data type with Priism, good reconstruction; see Mar 22 DPD notebook entry. stalked cell; large periplasmic space & S-layer bleb at non-stalk end; plaque-like object midway along convex side of cell; storage granule with denser partial coating; Im & PG involution midway along concave side of cell; clumps of dense material outside cell near membrane surface irregularitiesfilament segmented by Sarah
description: <strong>Tilt Series Date:</strong> 2005-10-04</p>
<strong>Data Taken By:</strong> Prabha Dias</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 0.5°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, bsoft, Priism</p>
<strong>Collaborators and Roles:</strong> specimen prep, data collection & processing: D. Prabha Dias</p>
<strong>Purification / Growth Conditions / Treatment:</strong> PYE media (RB Jensen protocol);  5ml cultures in 14ml tubes; exponential phase; see DPD notebook Sep 28-30, 2005</p>
<strong>Sample Preparation:</strong> box 642, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -1, blot 1 sec, drain 1 sec; see DPD notebook Sep 30, 2005</p>
  pdd2005-10-04-4_slicer4690.jpg: chemoreceptor array pdd2005-10-04-4_slicer4692.jpg: Granule pdd2005-10-04-4_slicer4694.jpg: S-layer pdd2005-10-04-4_slicer4695.jpg: S-layer two-layer
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2005-10-04-4/rawdata/</p>YB2845_05Oct04t04.mrc,  Tilt Series (Pixel Size 1.2 nm), 2.0 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        05Oct04t04.rec,  Reconstruction (Pixel Size 2.4 nm), 383.3 MB  
        
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        05Oct04t04_full.rec,  Reconstruction (Pixel Size 2.4 nm), 943.7 MB  
        
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        pd2005-10-04-4_clip01.rec,  Reconstruction (Pixel Size 2.4 nm) cropped by Sarah, 78.4 MB  
        
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        pd2005-10-04-4_clip01-label-Filament01.am,  filament segmented by Sarah, 627.7 kB  
        
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