DOI: 10.5061/dryad.x69p8czt5
title: Data from: Effects of female-specific selection for reproductive investment on male fertility traits

description: Despite sharing an autosomal genome, the often divergent reproductive
 strategies of males and females cause selection to act in a sex-specific
 manner. Selection acting on one sex can have negative, positive, or
 neutral fitness consequences on the opposite sex. Here we test how
 female-limited selection on reproductive investment in Japanese quail
 (Coturnix japonica) affects male fertility-related traits. Despite there
 being no difference in the size of males’ testes from lines selected for
 high female reproductive investment (H-line) or low female reproductive
 investment (L-line), in both lines, the left testis had a greater volume
 of sperm-producing tissue. Since H-line females have a larger left-side
 restricted oviduct, this suggests a positive genetic correlation between
 male and female gonad function, and that internal testis structure is a
 target of sexual selection. However, despite H-line males having
 previously been found to have greater fertilisation success in a
 competitive scenario, we found little evidence of a difference between the
 lines in sperm number, motility, velocity, length, or the number of sperm
 that reached the ova. Pre-copulatory cues and/or the role of seminal fluid
 in sperm motility may thus be more likely to contribute to the H-line male
 fertilisation advantage in this species.
description: # Data from: Effects of female-specific selection for reproductive
 investment on male fertility traits
 [https://doi.org/10.5061/dryad.x69p8czt5](https://doi.org/10.5061/dryad.x69p8czt5) Data and Code Responsibility: Chloe Mason ## Description of the data and file structure CSV files with data on various testis and sperm traits measured from a population of Japanese quail selected for high and low female reproductive investment. Each CSV file has a corresponding README file that contains a summary of the data and a description of the column headings. Digital images of testes used to measure testis volume, testis cross-sections used to measure the volume of sperm-producing tissue and the density of seminiferous tubules, and sperm used to measure sperm morphology, are provided. Video recordings used for sperm velocity and motility measures are provided.  ## Code/Software R scripts containing all analyses used in the main text and Supplementary Material.  Workflow: Code in Dyrad includes 6 scripts. testes.R contains analysis of testis wet mass, dry mass, volume of sperm-producing tissue, and density of seminiferous tubules. Also contains calculations for FDR-corrected p-values for all analyses. conc.motility.R contains analysis on sperm concentration and sperm motility. velocity.R contains the analysis of sperm velocity. morphology.R contains the analysis of sperm morphology. PVL.R contains the analysis of the number of sperm to reach the PVL. plots.R contains scripts for creating the figures in the manuscript and Supplementary Material. Outputs and figures are labelled as they appear in the manuscript/Supplementary Material. Software: R Version 4.4.0 (2024-04-24) Platform: aarch64-apple-darwin20 Running under: mac0S Monterey 12.1