cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.647852.v1
title: CIL:35146

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.
DOI: 10.6084/m9.figshare.647853.v1
title: CIL:35147

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 and atl-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. Downregulation of atl-1 suppressed the P1 cell division delay phenotype of cdc-48, ufd-1, and npl-4 RNAi embryos, suggesting that the DNA replication checkpoint is responsible for the delayed P1 cell division. The series corresponds to experiments shown in Fig. 2A and Movie 6 of Mouysset et al. Movie is CIL 25633 and original data file is CIL 35147.
DOI: 10.6084/m9.figshare.647854.v1
title: CIL:35148

description: This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The series corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 7 of Mouysset et al. Movie is CIL 25634 and original data is CIL 35148.
DOI: 10.6084/m9.figshare.647848
title: CIL:35123

description: This time lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo expressing YFP::CDC-48 by DIC and fluorescence microscopy. YFP::CDC-48, like YFP::UFD-1 and YFP::NPL-4, are cytosolic during mitosis, but accumulate in the nucleus after its reformation. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1A and Fig. S1 and Movie 1 of Mouysset et al. Movie is CIL 25628 and original data file is CIL 35123.
DOI: 10.6084/m9.figshare.647849
title: CIL:35124

description: This time lapse series illustrates the early division up to the four-cell stage of a wild-type C. elegans embryo by DIC microscopy. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The series corresponds to experiments shown in Fig. 1B and Movie 2 of Mouysset et al. Movie is CIL 25629 and original data file is CIL 35124.
DOI: 10.6084/m9.figshare.647850
title: CIL:35125

description: This time lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 3 of Mouysset et al. Movie is CIL 25630 and original data file is CIL 35125.
DOI: 10.6084/m9.figshare.647847
title: CIL:34922

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles of Xenopusfibroblast lamellipodia double stained with TRITC-phalloidin (red) and either p21 (Arp 2/3) antibodies (green). The protein/actin ratio at the leading edge of the lamellipodium is high for Arp2/3 complex. Immuno-EM of the cell edge (CIL 34925) or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 primary antibody and 10-nm gold-conjugated secondary antibody reveal Arp2/3 complex at Y-junctions. Image corresponds to Figure 5a and a' from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647848.v1
title: CIL:35123

description: This time lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo expressing YFP::CDC-48 by DIC and fluorescence microscopy. YFP::CDC-48, like YFP::UFD-1 and YFP::NPL-4, are cytosolic during mitosis, but accumulate in the nucleus after its reformation. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1A and Fig. S1 and Movie 1 of Mouysset et al. Movie is CIL 25628 and original data file is CIL 35123.
DOI: 10.6084/m9.figshare.647850.v1
title: CIL:35125

description: This time lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 3 of Mouysset et al. Movie is CIL 25630 and original data file is CIL 35125.
DOI: 10.6084/m9.figshare.647845
title: CIL:28235

description: This simulation shows a dynamic molecular model of the bacterial cytoplasm, giving us a spectacular glimpse of the crowded conditions of the interior of a cell over a brief 15-microsecond time span. The model includes 50 of the most abundant types of macromolecules reported in Escherichia coli, for a total concentration of 275g/L. This steric energy simulation shows a model of macromolecular diffusion based on Brownian dynamics and steric interactions between the molecules. This can be compared the the full energy model that also includes electrostatic and hydrophobic interactions.
DOI: 10.6084/m9.figshare.647846
title: CIL:28783

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 (strain OD31) in which KNL-1 has been depleted by RNAi. Discrete foci of GFP signal within the nucleus coalesce along curvilinear chromosomes during prophase. After nuclear envelope breakdown, chromosomes fail to congress to the metaphase plate, but GFP-KNL-2 localization is evident. As seen after CeCENP-A depletion and CeCENP-C depletion (CIL 28779 and CIL 28782, respectively), foci of GFP-KNL-2 formed along the spindle after anaphase onset. The video is ∼11 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals (see Materials and methods), and corresponding data are shown in Fig. 3 in J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.
DOI: 10.6084/m9.figshare.647847.v1
title: CIL:34922

description: Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles of Xenopusfibroblast lamellipodia double stained with TRITC-phalloidin (red) and either p21 (Arp 2/3) antibodies (green). The protein/actin ratio at the leading edge of the lamellipodium is high for Arp2/3 complex. Immuno-EM of the cell edge (CIL 34925) or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 primary antibody and 10-nm gold-conjugated secondary antibody reveal Arp2/3 complex at Y-junctions. Image corresponds to Figure 5a and a' from J Cell Biol. 1999 May 31;145(5):1009-26.
DOI: 10.6084/m9.figshare.647849.v1
title: CIL:35124

description: This time lapse series illustrates the early division up to the four-cell stage of a wild-type C. elegans embryo by DIC microscopy. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The series corresponds to experiments shown in Fig. 1B and Movie 2 of Mouysset et al. Movie is CIL 25629 and original data file is CIL 35124.
DOI: 10.6084/m9.figshare.647843
title: CIL:25824

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Rat2 cells expressing soluble EGFP, were subjected to CALI to test for nonspecific laser-induced effects. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, no morphological changes were visualized, as as seen in this DIC time-lapse movie. This and CIL# 25823 are control movies for CIL# 25821, 25822. This movie is Movie4 in Supporting Information in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647844
title: CIL:28234

description: This simulation shows a dynamic molecular model of the bacterial cytoplasm, giving us a spectacular glimpse of the crowded conditions of the interior of a cell over a brief 15-microsecond time span. The model includes 50 of the most abundant types of macromolecules reported in Escherichia coli, for a total concentration of 275g/L. This full energy simulation shows a model of macromolecular diffusion based on Brownian dynamics and intermolecular interactions including electrostatic and hydrophobic interactions.
DOI: 10.6084/m9.figshare.647846.v1
title: CIL:28783

description: Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 (strain OD31) in which KNL-1 has been depleted by RNAi. Discrete foci of GFP signal within the nucleus coalesce along curvilinear chromosomes during prophase. After nuclear envelope breakdown, chromosomes fail to congress to the metaphase plate, but GFP-KNL-2 localization is evident. As seen after CeCENP-A depletion and CeCENP-C depletion (CIL 28779 and CIL 28782, respectively), foci of GFP-KNL-2 formed along the spindle after anaphase onset. The video is ∼11 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals (see Materials and methods), and corresponding data are shown in Fig. 3 in J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.
DOI: 10.6084/m9.figshare.647843.v1
title: CIL:25824

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Rat2 cells expressing soluble EGFP, were subjected to CALI to test for nonspecific laser-induced effects. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, no morphological changes were visualized, as as seen in this DIC time-lapse movie. This and CIL# 25823 are control movies for CIL# 25821, 25822. This movie is Movie4 in Supporting Information in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647844.v1
title: CIL:28234

description: This simulation shows a dynamic molecular model of the bacterial cytoplasm, giving us a spectacular glimpse of the crowded conditions of the interior of a cell over a brief 15-microsecond time span. The model includes 50 of the most abundant types of macromolecules reported in Escherichia coli, for a total concentration of 275g/L. This full energy simulation shows a model of macromolecular diffusion based on Brownian dynamics and intermolecular interactions including electrostatic and hydrophobic interactions.
DOI: 10.6084/m9.figshare.647845.v1
title: CIL:28235

description: This simulation shows a dynamic molecular model of the bacterial cytoplasm, giving us a spectacular glimpse of the crowded conditions of the interior of a cell over a brief 15-microsecond time span. The model includes 50 of the most abundant types of macromolecules reported in Escherichia coli, for a total concentration of 275g/L. This steric energy simulation shows a model of macromolecular diffusion based on Brownian dynamics and steric interactions between the molecules. This can be compared the the full energy model that also includes electrostatic and hydrophobic interactions.
DOI: 10.6084/m9.figshare.647840
title: CIL:25634

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 7 of Mouysset et al. Movie is CIL 25634 and original data is CIL 35148.
DOI: 10.6084/m9.figshare.647840.v1
title: CIL:25634

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 7 of Mouysset et al. Movie is CIL 25634 and original data is CIL 35148.
DOI: 10.6084/m9.figshare.647842
title: CIL:25823

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells expressing EGFP-CP, were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, the dynamic changes on the dorsal surface of the cell seen in KDR cells (lacking endogenous CP and rescued with EGFP-CP, CIL# 25821,25822) were not observed in this DIC time-lapse movie. This and CIL 25824 are control movies. This movie is Movie3 in Supporting Information and still images are in Fig 2B, bottom panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647838
title: CIL:25632

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.
DOI: 10.6084/m9.figshare.647841
title: CIL:25821

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells deficient in endogenous CP, but rescued with EGFP-CP (KDR cells), were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, KDR cells exhibited dynamic changes on the dorsal surface of the cell, forming numerous protrusions as seen in this DIC time-lapse movie. A movie of a zoomed area of the irradiated region from this cell is in CIL# 25822 and control movies are CIL#s 25823, 25824. This movie is Movie1 in Supporting Information and still images are in Fig 2B, top panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647842.v1
title: CIL:25823

description: Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells expressing EGFP-CP, were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, the dynamic changes on the dorsal surface of the cell seen in KDR cells (lacking endogenous CP and rescued with EGFP-CP, CIL# 25821,25822) were not observed in this DIC time-lapse movie. This and CIL 25824 are control movies. This movie is Movie3 in Supporting Information and still images are in Fig 2B, bottom panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.
DOI: 10.6084/m9.figshare.647836
title: CIL:25630

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 3 of Mouysset et al. Movie is CIL 25630 and original data file is CIL 35125.
DOI: 10.6084/m9.figshare.647837
title: CIL:25631

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of ufd-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 4 of Mouysset et al. Movie is CIL 25631 and original data file is CIL 35126.
DOI: 10.6084/m9.figshare.647837.v1
title: CIL:25631

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of ufd-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 4 of Mouysset et al. Movie is CIL 25631 and original data file is CIL 35126.
DOI: 10.6084/m9.figshare.647838.v1
title: CIL:25632

description: This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The movie corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.