DOI: 10.5061/dryad.gqnk98skz
title: Influence of polymorphisms in the vascular endothelial growth factor gene on allograft rejection after kidney transplantation: a meta-analysis

description: Purpose Reported associations of allograft rejection in kidney transplant
 patients with VEGF single nucleotide polymorphisms (SNPs) have been
 inconsistent between studies, which prompted a meta-analysis to obtain
 more precise estimates. Methods Using the PICO elements, kidney transplant
 patients (P) were compared by genotype data between rejectors (I) and
 non-rejectors (C) in order to determine the risk of allograft rejection
 (O) attributed to the VEGF SNPs. Literature search of four databases
 yielded seven articles. To calculate risks for allograft rejection, four
 SNPs were examined. Meta-analysis treatments included outlier and subgroup
 analyses, the latter was based on ethnicity (Indians/Caucasians) and
 rejection type (acute/chronic). Multiple comparisons were corrected with
 the Bonferroni test. Results Five highly significant outcomes (Pa &lt;
 0.01) survived Bonferroni correction, one of which showed reduced risk for
 the var allele (OR 0.61, 95% CI 0.45-0.82). The remaining four indicated
 increased risk for the wt allele where the chronic rejection (OR 2.10, 95%
 CI 1.36-3.24) and Indian (OR 1.44, 95% CI 1.13-1.84) subgroups were
 accorded susceptibility status. Conclusions Risk associations for renal
 allograft rejection were increased and reduced on account of the wt and
 var alleles, respectively. These findings could render the VEGF
 polymorphisms useful in the clinical genetics of kidney transplantation.
description: From published articles
description: Genetics of renal transplantation. There are
 no missing values. <i>VEGF</i> dataset used
 to generate summary effects and confidence intervals for the figures and
 tables of the manuscript   First
 author 
 Ethnicity AR/
 CR 
 <i>VEGF</i> SNPs      
 Sample sizes Rejection 
 Non-rejection
 minor allele frequency
 
           Rejection 
 Non- rejection 
 Total <i>wt-wt</i>
 <i>wt-var</i> <i>var-var</i> <i>wt-wt</i> <i>wt-var</i> <i>var-var</i>     <b>Publication Datasets</b>                         1 Jimenez-Sousa Caucasian CR rs699947 158 118 276 40 83 35 45 49 24 0.41 2 Lemos Caucasian AR rs699947 93 267 360 21 46 26 60 133 74 0.53 3 Shahbazi Caucasian AR rs699947 64 103 167 24 33 7 24 50 29 0.52 4 Mittal Indian AR rs699947 du du ---- 10 23 11 30 71 55 0.58 5 Prakash 2015 Indian AR rs699947 76 196 272 23 31 22 38 119 39 0.50 6 Prakash 2015 Indian AR rs144854329 du du ---- 19 34 23 39 101 56 0.59 7 Prakash 2018 Indian CR rs699947 98 174 272 13 52 33 48 98 28 0.44 8 Prakash 2018 Indian CR rs144854329 du du ---- 15 62 21 43 73 58 0.54 9 Prakash 2018 Indian AR rs699947 du du ---- 48 98 28 13 52 33 0.60 10 Prakash 2018 Indian AR rs144854329 54 218 272 58 73 43 21 62 15 0.47 1 Lemos Caucasian AR rs1570360 du du ---- 47 38 8 118 119 30 0.34 2 Shahbazi Caucasian AR rs1570360 61 98 159 33 25 3 34 43 21 0.43 3 Mittal Indian AR rs1570360 44 156 200 13 16 15 48 51 57 0.53 4 Prakash 2015 Indian AR rs1570360 du du ---- 27 31 18 35 115 46 0.53 5 Prakash 2018 Indian CR rs1570360 du du ---- 23 53 22 39 93 42 0.51 1 Gunesacar Caucasian AR rs3025039 265 290 555 231 31 3 230 55 5 0.11 2 Prakash 2015 Indian AR rs3025039 du du ---- 20 33 23 79 80 37 0.39 3 Prakash 2018 Indian CR rs3025039 du du ---- 39 42 17 60 71 43 0.45                              <i>VEGF</i>: <i>vascular endothelial growth factor</i> polymorphisms; AR: acute rejection; CR: chronic rejection; SNPs: single nucleotide                polymorphisms;<i> wt</i>: wild-type; <i>var</i>: variant; du: duplicate   <b>Publication references</b> Jimenez-Sousa MA, Fernandez-Rodriguez A, Heredia M, Tamayo E, Guzman-Fulgencio M, Lajo C, Lopez E, Gomez-Herreras JI, Bustamante J, Bermejo-Martin JF, Resino S (2012) Genetic polymorphisms located in TGFB1, AGTR1, and VEGFA genes are associated to chronic renal allograft dysfunction. Cytokine 58 (3):321-326. doi:10.1016/j.cyto.2012.02.017 Lemos FB, Mol WM, Roodnat JI, Uitterlinden A, Ijzermans JN, Weimar W, Baan CC (2005) The beneficial effects of recipient-derived vascular endothelial growth factor on graft survival after kidney transplantation. Transplantation 79 (9):1221-1225. doi:10.1097/01.tp.0000161219.75906.ec Shahbazi M, Fryer AA, Pravica V, Brogan IJ, Ramsay HM, Hutchinson IV, Harden PN (2002) Vascular endothelial growth factor gene polymorphisms are associated with acute renal allograft rejection. Journal of the American Society of Nephrology : JASN 13 (1):260-264 Mittal RD, Srivastava P, Singh V, Jaiswal P, Kapoor R (2011) Association of common variants of vascular endothelial growth factor and interleukin-18 genes with allograft survival in renal transplant recipients of North India. DNA and cell biology 30 (5):309-315. doi:10.1089/dna.2010.1138 Prakash S, Agrawal S, Kumar S, Prasad N (2015) Impact of vascular endothelial growth factor single nucleotide polymorphism association on acute renal allograft rejection. Nephron 129 (2):91-96. doi:10.1159/000368700 Prakash S, Patel MR, Agrawal S, Jindal RM, Prasad N (2018) Vascular Endothelial Growth Factor Gene Polymorphism Is Associated With Long-term Kidney Allograft Outcomes. Kidney international reports 3 (2):321-327. doi:10.1016/j.ekir.2017.10.008 Gunesacar R, Opelz G, Erken E, Pelzl S, Dohler B, Ruhenstroth A, Susal C (2007) VEGF 936 C/T gene polymorphism in renal transplant recipients: association of the T allele with good graft outcome. Human immunology 68 (7):599-602. doi:10.1016/j.humimm.2007.03.015