DOI: 10.5061/dryad.bk3j9kdps
title: Data from: <em>Htr1b</em> is necessary for normal retinal function in mice

description: Introduction: Serotonin (5-HT) is a neurotransmitter that is involved in
 retinal development, physiology, and vision, yet the specific contribution
 of individual 5-HT receptors to retinal function is poorly characterized.
 We identified 5-HT receptor 1B (Htr1b) as a potential key regulator of
 serotonergic signaling in the retina. Methods: Htr1b localization was
 examined using RNAseq and in situ labeling. Retinal structure was assessed
 using histology and SD-OCT. Visual function was evaluated using optomotor
 behavioral experiments. Retinal function was characterized in vivo using
 electroretinography (ERG) and ex vivo using multielectrode array (MEA)
 recordings. Results: Htr1b transcript and HTR1B protein localized
 primarily to the inner retina and RGCs. While Htr1b-/- mice displayed
 normal retinal anatomy, they exhibited visual deficits in contrast
 sensitivity and visual acuity. ERG recordings revealed that RGCs had
 latency delays and reduced sensitivity to changes in light intensity. MEA
 analysis showed altered RGC firing patterns and increased variability
 following 5-HT application. These effects were cell-type specific:
 Htr1b-/- ON RGCs showed elevated basal firing rates while Htr1b-/- OFF
 RGCs showed reduced 5-HT responses. Discussion: These findings demonstrate
 that Htr1b is necessary for normal retinal serotonergic signaling and
 contributes to the regulation of RGC excitability and visual sensitivity.
description: # Data from: *Htr1b* is necessary for normal retinal function in mice
 Dataset DOI:
 [10.5061/dryad.bk3j9kdps](https://doi.org/10.5061/dryad.bk3j9kdps) ##
 **Description of files** ### Retinal_thickness_measurements_FINAL.xlsx
 **Retinal Thickness Measurements** Sheet:
 ‘Retinal_thickness_measurements_FINAL’ Contains mean and individual
 retinal layer thickness values for each genotype. Tab 1: ‘Histology’
 Retinal layer thickness measurements obtained from histological sections.
 **Column Title - Meaning** GCL - Ganglion Cell Layer IPL - Inner Plexiform
 Layer INL - Inner Nuclear Layer OPL - Outer Plexiform Layer ONL - Outer
 Nuclear Layer Units: µm   Tab 2: ‘SD-OCT’ Retinal thickness measurements
 obtained using SD-OCT imaging. **Column Title - Meaning** RNFL - Retinal
 Nerve Fiber Layer GCL/IPL - -Combined Ganglion Cell + Inner Plexiform
 Layers INL - Inner Nuclear Layer OPL - Outer Plexiform Layer ONL - Outer
 Nuclear Layer ELM/IS/OS - External Limiting Membrane / Inner Segments /
 Outer Segments (combined region) Units: mm   ### OKR_analysis_FINAL.xlsx
 **OKR Analysis** Sheet: ’OKR_analysis_FINAL’ Contains optomotor reflex
 (OKR) measurements. Tab 1: ‘CS Threshold Photopic’ Contrast sensitivity
 thresholds under photopic conditions. Contrast sensitivity is reported as
 a dimensionless value. Tab 2: ‘CS Threshold Scotopic’ Contrast sensitivity
 thresholds under scotopic conditions. Contrast sensitivity is reported as
 a dimensionless value. Tab 3: ‘CS Function’ Contrast sensitivity
 measurements across 5 spatial frequencies. Unit: cycles per degree
 (cyc/deg). Tab 4: ‘Spatial Freq Threshold’ Spatial frequency threshold
 measurements. Units: cyc/deg ### ERG_values_FINAL.xlsx
 **Electroretinogram** Sheet: ‘ERG_values_FINAL’ Contains ERG amplitudes
 across a range of flash intensities.\ Flash intensity units:
 photoisomerizations per rod (R*). Tab 1: ‘pSTR Amplitude’ pSTR amplitudes
 from WT and *Htr1b*-/- retinas across multiple intensities.\ Amplitude
 units: microvolts (µV) Tab 2: ‘B-wave Amplitude’ B-wave amplitudes from WT
 and Htr1b-/- retinas across multiple intensities.\ Amplitude units:
 microvolts (µV) Tab 3: ‘ERG slope analysis pSTR’ Slope of pSTR amplitude
 plotted as a function of flash intensity.\ Units: µV per R* (µV/R*) Tab 4:
 ‘ERG slope analysis B wave’ Same analysis as Tab 3, using b-wave
 amplitudes.   ### MEA_data.xlsx **Multielectrode Array** Sheet: ‘MEA_data’
 Contains multielectrode array spike rate and 5-HT response data. Tab 1:
 Master table for organizing RGCs. Unit - Electrode identifier Retina
 - Retina identifier Genotype - WT or *Htr1b*-/- Response - Increase or
 decrease firing rate in response to 5-HT ON-OFF - ON, OFF, or ON-OFF cell
 type Tabs 2-9: RGCs grouped by: 1.      ON vs OFF classification 2.     
 Increased vs decreased firing after 5-HT 3.      Baseline (bath) vs 5-HT
 conditions Each table lists spike rate (Hz) binned at 1-second intervals.
 **Note**:Throughout all files, yellow-filled cells represent missing or
 excluded data. ## Access information Other publicly accessible locations
 of the data: * None Data was derived from the following sources: * n/a