cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.4239728.v1
title: Evidence-Based Guidelines for Fatigue Risk Management in EMS: Formulating Research Questions and Selecting Outcomes

description: <b>Background</b>: Greater than half of Emergency Medical Services (EMS) personnel report work-related fatigue, yet there are no guidelines for the management of fatigue in EMS. A novel process has been established for evidence-based guideline (EBG) development germane to clinical EMS questions. This process has not yet been applied to operational EMS questions like fatigue risk management. The objective of this study was to develop content valid research questions in the Population, Intervention, Comparison, and Outcome (PICO) framework, and select outcomes to guide systematic reviews and development of EBGs for EMS fatigue risk management. <b>Methods</b>: We adopted the National Prehospital EBG Model Process and Grading of Recommendations Assessment, Development, and Evaluation (GRADE) framework for developing, implementing, and evaluating EBGs in the prehospital care setting. In accordance with steps one and two of the Model Process, we searched for existing EBGs, developed a multi-disciplinary expert panel and received external input. Panelists completed an iterative process to formulate research questions. We used the Content Validity Index (CVI) to score relevance and clarity of candidate PICO questions. The panel completed multiple rounds of question editing and used a CVI benchmark of ≥0.78 to indicate acceptable levels of clarity and relevance. Outcomes for each PICO question were rated from 1 = less important to 9 = critical. <b>Results</b>: Panelists formulated 13 candidate PICO questions, of which 6 were eliminated or merged with other questions. Panelists reached consensus on seven PICO questions (<i>n</i> = 1 diagnosis and <i>n</i> = 6 intervention). Final CVI scores of relevance ranged from 0.81 to 1.00. Final CVI scores of clarity ranged from 0.88 to 1.00. The mean number of outcomes rated as critical, important, and less important by PICO question was 0.7 (SD 0.7), 5.4 (SD 1.4), and 3.6 (SD 1.9), respectively. Patient and personnel safety were rated as critical for most PICO questions. PICO questions and outcomes were registered with PROSPERO, an international database of prospectively registered systematic reviews. <b>Conclusions</b>: We describe formulating and refining research questions and selection of outcomes to guide systematic reviews germane to EMS fatigue risk management. We outline a protocol for applying the Model Process and GRADE framework to create evidence-based guidelines
DOI: 10.6084/m9.figshare.4239716
title: Vascular ultrasound measures before pregnancy and pregnancy complications: A prospective cohort study

description: <i>Objectives</i>: To examine the relationship between pre-pregnancy indicators of cardiovascular risk and pregnancy complications and outcomes. <i>Study design</i>: Data from 359 female participants in the Cardiovascular Risk in Young Finns Study were linked with the national birth registry. Flow-mediated dilatation (FMD; maximum change in the left brachial artery diameter after rest and hyperemia); carotid intima-media thickness (IMT); Young’s elastic modulus (YEM); and carotid artery distensibility (Cdist) at the visit prior to the pregnancy were examined as predictors of hypertensive disorders, birthweight, and gestational age using multivariable linear regression with adjustment for confounders (age, BMI, smoking, and socioeconomic status). <i>Results</i>: No relations were seen between FMD, IMT, or the stiffness indices, and hypertensive disorders. Higher pre-pregnancy FMD was associated with lower gestational age, while increased Cdist was associated with reduced birthweight-for-gestational-age. <i>Conclusions</i>: Some cardiovascular ultrasound measures of pre-pregnancy may predict pregnancy complications, but the association is likely to be small.
DOI: 10.6084/m9.figshare.4239716.v1
title: Vascular ultrasound measures before pregnancy and pregnancy complications: A prospective cohort study

description: <i>Objectives</i>: To examine the relationship between pre-pregnancy indicators of cardiovascular risk and pregnancy complications and outcomes. <i>Study design</i>: Data from 359 female participants in the Cardiovascular Risk in Young Finns Study were linked with the national birth registry. Flow-mediated dilatation (FMD; maximum change in the left brachial artery diameter after rest and hyperemia); carotid intima-media thickness (IMT); Young’s elastic modulus (YEM); and carotid artery distensibility (Cdist) at the visit prior to the pregnancy were examined as predictors of hypertensive disorders, birthweight, and gestational age using multivariable linear regression with adjustment for confounders (age, BMI, smoking, and socioeconomic status). <i>Results</i>: No relations were seen between FMD, IMT, or the stiffness indices, and hypertensive disorders. Higher pre-pregnancy FMD was associated with lower gestational age, while increased Cdist was associated with reduced birthweight-for-gestational-age. <i>Conclusions</i>: Some cardiovascular ultrasound measures of pre-pregnancy may predict pregnancy complications, but the association is likely to be small.
DOI: 10.6084/m9.figshare.4239686
title: Toxins MazF and MqsR cleave <i>Escherichia coli</i> rRNA precursors at multiple sites

description: The endoribonuclease toxins of the <i>E. coli</i> toxin-antitoxin systems arrest bacterial growth and protein synthesis by targeting cellular mRNAs. As an exception, <i>E. coli</i> MazF was reported to cleave also 16S rRNA at a single site and separate an anti-Shine-Dalgarno sequence-containing RNA fragment from the ribosome. We noticed extensive rRNA fragmentation in response to induction of the toxins MazF and MqsR, which suggested that these toxins can cleave rRNA at multiple sites. We adapted differential RNA-sequencing to map the toxin-cleaved 5′- and 3′-ends. Our results show that the MazF and MqsR cleavage sites are located within structured rRNA regions and, therefore, are not accessible in assembled ribosomes. Most of the rRNA fragments are located in the aberrant ribosomal subunits that accumulate in response to toxin induction and contain unprocessed rRNA precursors. We did not detect MazF- or MqsR-cleaved rRNA in stationary phase bacteria and in assembled ribosomes. Thus, we conclude that MazF and MqsR cleave rRNA precursors before the ribosomes are assembled and potentially facilitate the decay of surplus rRNA transcripts during stress.
DOI: 10.6084/m9.figshare.4239686.v2
title: Toxins MazF and MqsR cleave <i>Escherichia coli</i> rRNA precursors at multiple sites

description: The endoribonuclease toxins of the <i>E. coli</i> toxin-antitoxin systems arrest bacterial growth and protein synthesis by targeting cellular mRNAs. As an exception, <i>E. coli</i> MazF was reported to cleave also 16S rRNA at a single site and separate an anti-Shine-Dalgarno sequence-containing RNA fragment from the ribosome. We noticed extensive rRNA fragmentation in response to induction of the toxins MazF and MqsR, which suggested that these toxins can cleave rRNA at multiple sites. We adapted differential RNA-sequencing to map the toxin-cleaved 5′- and 3′-ends. Our results show that the MazF and MqsR cleavage sites are located within structured rRNA regions and, therefore, are not accessible in assembled ribosomes. Most of the rRNA fragments are located in the aberrant ribosomal subunits that accumulate in response to toxin induction and contain unprocessed rRNA precursors. We did not detect MazF- or MqsR-cleaved rRNA in stationary phase bacteria and in assembled ribosomes. Thus, we conclude that MazF and MqsR cleave rRNA precursors before the ribosomes are assembled and potentially facilitate the decay of surplus rRNA transcripts during stress.
DOI: 10.6084/m9.figshare.4239677
title: Required Peer Cooperative Learning STEM Retention Data

description: Data on student success and retention-in-major (DVs) for a sample of science and mathematics majors before and after a required peer-cooperative learning program was implemented. IVs include student major, course taken, SAT scores, and demographic data including gender, low-income student status, student-of-color status, and first-generation college student status.
DOI: 10.6084/m9.figshare.4239686.v1
title: Toxins MazF and MqsR cleave <i>Escherichia coli</i> ribosomal RNA precursors at multiple sites

description: The endoribonuclease toxins of the <i>E. coli</i> toxin-antitoxin systems arrest bacterial growth and protein synthesis by targeting cellular mRNAs. As an exception, <i>E. coli</i> MazF was reported to cleave also 16S rRNA at a single site and separate an anti-Shine-Dalgarno sequence-containing RNA fragment from the ribosome. We noticed extensive rRNA fragmentation in response to induction of the toxins MazF and MqsR, which suggested that these toxins can cleave rRNA at multiple sites. We adapted differential RNA-sequencing to map the toxin-cleaved 5′- and 3′-ends. Our results show that the MazF and MqsR cleavage sites are located within structured rRNA regions and, therefore, are not accessible in assembled ribosomes. Most of the rRNA fragments are located in the aberrant ribosomal subunits that accumulate in response to toxin induction and contain unprocessed rRNA precursors. We did not detect MazF- or MqsR-cleaved rRNA in the stationary phase bacteria and in assembled ribosomes. Thus, we conclude that MazF and MqsR cleave rRNA precursors before the ribosomes are assembled and potentially facilitate the decay of surplus rRNA transcripts during stress.
DOI: 10.6084/m9.figshare.4239674
title: High-throughput sequencing of two populations of extracellular vesicles provides an mRNA signature that can be detected in the circulation of breast cancer patients

description: Extracellular vesicles (EVs) contain a wide range of RNA types with a reported prevalence of non-coding RNA. To date a comprehensive characterization of the protein coding transcripts in EVs is still lacking. We performed RNA-Sequencing (RNA-Seq) of 2 EV populations and identified a small fraction of transcripts that were expressed at significantly different levels in large oncosomes and exosomes, suggesting they may mediate specialized functions. However, these 2 EV populations exhibited a common mRNA signature that, in comparison to their donor cells, was significantly enriched in mRNAs encoding E2F transcriptional targets and histone proteins. These mRNAs are primarily expressed in the S-phase of the cell cycle, suggesting that they may be packaged into EVs during S-phase. <i>In silico</i> analysis using subcellular compartment transcriptome data from the ENCODE cell line compendium revealed that EV mRNAs originate from a cytoplasmic RNA pool. The EV signature was independently identified in plasma of patients with breast cancer by RNA-Seq. Furthermore, several transcripts differentially expressed in EVs from patients versus controls mirrored differential expression between normal and breast cancer tissues. Altogether, this largest high-throughput profiling of EV mRNA demonstrates that EVs carry tumor-specific alterations and can be interrogated as a source of cancer-derived cargo.
DOI: 10.6084/m9.figshare.4239671
title: A Pliocene mekosuchine (Eusuchia: Crocodilia) from the Lake Eyre Basin of South Australia

description: A new genus and species of crocodilian, <i>Kalthifrons aurivellensis</i>, is described from a channel sand deposit incised into the Oligo-Miocene Etadunna Formation on the western shore of Lake Palankarinna in the Lake Eyre Basin, South Australia. The channel sand is interpreted as an outcrop of the Mampuwordu Sand Member of the Tirari Formation, which has been assigned an early Pliocene age. The taxon can be diagnosed by the extremely elongate and narrow anterior process of the frontal pair and a distinctively beveled and laterally expanded prefrontal contribution to the orbit margin. The broadly triangular rostrum, at least partially interlocking dentition, smooth dental carinae, and weak labiolingual compression resemble unspecialized crocodilian species, and it was probably a generalist aquatic predator. It possessed character states that indicate that it was not a member of <i>Crocodylus</i>, and it is referred to the endemic Australasian clade Mekosuchinae. The first overlying unit in the Lake Eyre Basin sequence to produce diagnostic crocodilian remains is the mid- to late Pliocene Pompapillina Member of the Tirari Formation. It contains a true species of <i>Crocodylus</i> but no indication of <i>Kalthifrons aurivellensis</i> or indeed any other mekosuchine, indicating that the latter may have been driven to extinction via competitive replacement. http://zoobank.org/urn:lsid:zoobank.org:pub:C4E93358-D80F-4E4D-B7FF-BFFB93EDCD54 SUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVP Citation for this article: Yates, A. M., and N. S. Pledge. 2016. A Pliocene mekosuchine (Eusuchia: Crocodilia) from the Lake Eyre Basin of South Australia. Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1244540.
DOI: 10.6084/m9.figshare.4239671.v1
title: A Pliocene mekosuchine (Eusuchia: Crocodilia) from the Lake Eyre Basin of South Australia

description: A new genus and species of crocodilian, <i>Kalthifrons aurivellensis</i>, is described from a channel sand deposit incised into the Oligo-Miocene Etadunna Formation on the western shore of Lake Palankarinna in the Lake Eyre Basin, South Australia. The channel sand is interpreted as an outcrop of the Mampuwordu Sand Member of the Tirari Formation, which has been assigned an early Pliocene age. The taxon can be diagnosed by the extremely elongate and narrow anterior process of the frontal pair and a distinctively beveled and laterally expanded prefrontal contribution to the orbit margin. The broadly triangular rostrum, at least partially interlocking dentition, smooth dental carinae, and weak labiolingual compression resemble unspecialized crocodilian species, and it was probably a generalist aquatic predator. It possessed character states that indicate that it was not a member of <i>Crocodylus</i>, and it is referred to the endemic Australasian clade Mekosuchinae. The first overlying unit in the Lake Eyre Basin sequence to produce diagnostic crocodilian remains is the mid- to late Pliocene Pompapillina Member of the Tirari Formation. It contains a true species of <i>Crocodylus</i> but no indication of <i>Kalthifrons aurivellensis</i> or indeed any other mekosuchine, indicating that the latter may have been driven to extinction via competitive replacement. http://zoobank.org/urn:lsid:zoobank.org:pub:C4E93358-D80F-4E4D-B7FF-BFFB93EDCD54 SUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVP Citation for this article: Yates, A. M., and N. S. Pledge. 2016. A Pliocene mekosuchine (Eusuchia: Crocodilia) from the Lake Eyre Basin of South Australia. Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1244540.
DOI: 10.6084/m9.figshare.4239674.v1
title: High-throughput sequencing of two populations of extracellular vesicles provides an mRNA signature that can be detected in the circulation of breast cancer patients

description: Extracellular vesicles (EVs) contain a wide range of RNA types with a reported prevalence of non-coding RNA. To date a comprehensive characterization of the protein coding transcripts in EVs is still lacking. We performed RNA-Sequencing (RNA-Seq) of 2 EV populations and identified a small fraction of transcripts that were expressed at significantly different levels in large oncosomes and exosomes, suggesting they may mediate specialized functions. However, these 2 EV populations exhibited a common mRNA signature that, in comparison to their donor cells, was significantly enriched in mRNAs encoding E2F transcriptional targets and histone proteins. These mRNAs are primarily expressed in the S-phase of the cell cycle, suggesting that they may be packaged into EVs during S-phase. <i>In silico</i> analysis using subcellular compartment transcriptome data from the ENCODE cell line compendium revealed that EV mRNAs originate from a cytoplasmic RNA pool. The EV signature was independently identified in plasma of patients with breast cancer by RNA-Seq. Furthermore, several transcripts differentially expressed in EVs from patients versus controls mirrored differential expression between normal and breast cancer tissues. Altogether, this largest high-throughput profiling of EV mRNA demonstrates that EVs carry tumor-specific alterations and can be interrogated as a source of cancer-derived cargo.
DOI: 10.6084/m9.figshare.4239608
title: Tracking the evolution of epialleles during neural differentiation and brain development: <i>D-Aspartate oxidase</i> as a model gene

description: We performed ultra-deep methylation analysis at single molecule level of the promoter region of developmentally regulated <i>D-Aspartate oxidase</i> (<i>Ddo</i>), as a model gene, during brain development and embryonic stem cell neural differentiation. Single molecule methylation analysis enabled us to establish the effective epiallele composition within mixed or pure brain cell populations. In this framework, an epiallele is defined as a specific combination of methylated CpG within <i>Ddo</i> locus and can represent the epigenetic haplotype revealing a cell-to-cell methylation heterogeneity. Using this approach, we found a high degree of polymorphism of methylated alleles (epipolymorphism) evolving in a remarkably conserved fashion during brain development. The different sets of epialleles mark stage, brain areas, and cell type and unravel the possible role of specific CpGs in favoring or inhibiting local methylation. Undifferentiated embryonic stem cells showed non-organized distribution of epialleles that apparently originated by stochastic methylation events on individual CpGs. Upon neural differentiation, despite detecting no changes in average methylation, we observed that the epiallele distribution was profoundly different, gradually shifting toward organized patterns specific to the glial or neuronal cell types. Our findings provide a deep view of gene methylation heterogeneity in brain cell populations promising to furnish innovative ways to unravel mechanisms underlying methylation patterns generation and alteration in brain diseases.
DOI: 10.6084/m9.figshare.4239608.v1
title: Tracking the evolution of epialleles during neural differentiation and brain development: <i>D-Aspartate oxidase</i> as a model gene

description: We performed ultra-deep methylation analysis at single molecule level of the promoter region of developmentally regulated <i>D-Aspartate oxidase</i> (<i>Ddo</i>), as a model gene, during brain development and embryonic stem cell neural differentiation. Single molecule methylation analysis enabled us to establish the effective epiallele composition within mixed or pure brain cell populations. In this framework, an epiallele is defined as a specific combination of methylated CpG within <i>Ddo</i> locus and can represent the epigenetic haplotype revealing a cell-to-cell methylation heterogeneity. Using this approach, we found a high degree of polymorphism of methylated alleles (epipolymorphism) evolving in a remarkably conserved fashion during brain development. The different sets of epialleles mark stage, brain areas, and cell type and unravel the possible role of specific CpGs in favoring or inhibiting local methylation. Undifferentiated embryonic stem cells showed non-organized distribution of epialleles that apparently originated by stochastic methylation events on individual CpGs. Upon neural differentiation, despite detecting no changes in average methylation, we observed that the epiallele distribution was profoundly different, gradually shifting toward organized patterns specific to the glial or neuronal cell types. Our findings provide a deep view of gene methylation heterogeneity in brain cell populations promising to furnish innovative ways to unravel mechanisms underlying methylation patterns generation and alteration in brain diseases.
DOI: 10.6084/m9.figshare.4239602
title: Convenient synthesis, antimicrobial evaluation and molecular modeling of some novel quinoline derivatives

description: <i>α, β</i>-Unsaturated carbonyl compounds <b>2a, 2b, 3,</b> and <b>4</b> were synthesized by the Knoevenagel condensation between 2-substituted quionoline-3-carboxaldehyde <b>1a</b> and/or <b>1b</b> with active methylene compounds. In addition, the synthesis of azlactone is achieved starting from <b>1a</b> and <i>N</i>-acetylglycine. Synthesis of pyridine, pyrene, and pyrimidine derivatives <b>6</b>–<b>8</b> were accomplished via one-pot multicomponent reaction of <b>1b</b> with acetyl acetone, malononitrile, and ammonium acetate; acetophenone, malononitrile, and NaOH; or acetyl acetone and urea in acidic medium. The new synthesized compounds showed good antimicrobial activities. The DFT calculations have been used to predict the electronic properties of the studied compounds.
DOI: 10.6084/m9.figshare.4239602.v2
title: Convenient synthesis, antimicrobial evaluation and molecular modeling of some novel quinoline derivatives

description: <i>α, β</i>-Unsaturated carbonyl compounds <b>2a, 2b, 3,</b> and <b>4</b> were synthesized by the Knoevenagel condensation between 2-substituted quionoline-3-carboxaldehyde <b>1a</b> and/or <b>1b</b> with active methylene compounds. In addition, the synthesis of azlactone is achieved starting from <b>1a</b> and <i>N</i>-acetylglycine. Synthesis of pyridine, pyrene, and pyrimidine derivatives <b>6</b>–<b>8</b> were accomplished via one-pot multicomponent reaction of <b>1b</b> with acetyl acetone, malononitrile, and ammonium acetate; acetophenone, malononitrile, and NaOH; or acetyl acetone and urea in acidic medium. The new synthesized compounds showed good antimicrobial activities. The DFT calculations have been used to predict the electronic properties of the studied compounds.
DOI: 10.6084/m9.figshare.4239602.v1
title: A Convenient Synthesis, Antimicrobial Evaluation and Molecular Modeling of Some Novel Quinoline Derivatives

description: α, β-unsaturated carbonyl compounds<b>2a, b, 3</b> and <b>4</b> were synthesized by the Knoevenagel condensation between 2-substitutedquionoline-3-carboxaldehyde <b>1a</b> and/or <b>1b</b> with active methylene compounds. In addition, the synthesis of azlactone is achieved starting from <b>1a</b> and <i>N</i>-acetylglycine. Synthesis of pyridine, pyrane and pyrimidine derivatives <b>6</b>-<b>8</b> were accomplished via one-pot multicomponent reaction of <b>1b</b> with (acetyl acetone, malononitrile and ammonium acetate), (acetophenone, malononitrile and NaOH) or (acetyl acetone and urea in acidic medium). The new synthesized compounds showed good antimicrobial activities. The DFT calculations have been used to predict the electronic properties of the studied compounds.
DOI: 10.6084/m9.figshare.4239512
title: 上证50.xlsx

description: 上证指数的五十家公司股票情况