cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14276049.v1
title: Multifractal analysis of soil fauna diversity indexes

description: ABSTRACT The objective of this study was to determine the multifractality of diversity indexes of edaphic fauna in areas with natural vegetation and in agricultural systems. Biological sampling was carried out in seven treatments (millet, maize, soybean, eucalyptus, preserved cerrado, disturbed cerrado and pasture), containing 130 pitfall traps, distributed in transects with 3 m of spacing between sampling points, totaling 390 m. The multifractal analysis was determined based on the moment method, where estimates such as the capacity dimensions, entropy and the correlation of the diversity indexes were calculated. The soybean area had greater Shannon diversity (2.69), however it had smaller abundance of individuals. The partition functions were adjusted with coefficient of determination &gt; 0.90. The difference between D-10-D10, ranged from 0.080 to 1,707 for Pielou equitability in soybean cultivation, for richness in the area under the cultivation of eucalyptus. The singularity spectra expressed graphs with different degrees of heterogeneity for the soil fauna indexes, and the richness expressed the best structure. The area cultivated with soybean had a monofractal tendency, due to the homogeneous distribution of individuals of the edaphic fauna along the transect. The fractal analysis provided the description of patterns of variability that are not detected by classical methods.
DOI: 10.6084/m9.figshare.14276055
title: Greenhouse gas emission, water quality and straw decomposition as a function of rice postharvest field management

description: ABSTRACT The objective of this paper was to evaluate the quality of water drained after the use of the knife-roller (an implement used to incorporate rice residues after harvest), the partial global warming potential (pGWP) and straw decomposition as a result of postharvest field management of irrigated rice. The experiments were conducted during the 2018 and 2019 offseason and the treatments consisted of several water drainage periods (0, 3, 6, 9 and 12 days) after a field pass with a knife-roller. In addition, a nonflooded harvest treatment without straw management was included. Analysis of drainage water three days after a pass with the kniferoller showed a reduction in total soluble solids by 94% compared to zero days. Nitrogen and soluble phosphorus were not influenced by the treatments. However, potassium levels increased as the drainage period increased, which is related to the period between harvest and drainage. As the electrical conductivity is influenced by the concentration of ions, it showed the same response curve as the potassium levels and, for pH, there were small oscillations influenced by the temperature. The total CH4 emission and the pGWP were higher when the water depth remained for a longer period. However, the N2O emissions were higher in the absence of soil submersion. Water should be drained three days after a pass with the knife-roller in order to reduce potassium loss and suspended solids as well as pGWP. The use of the knife-roller accelerated the process of straw decomposition in relation to the unmanaged straw treatment.
DOI: 10.6084/m9.figshare.14276057
title: Selection to high productivity and stink bugs resistance by multivariate data analyses in soybean

description: ABSTRACT Stink bugs that affect soybeans are responsible for significant losses in seed production, quality and germination potential, in addition to hindering the mechanized harvest. To develop insect resistant materials, the breeder can compile a selection index by factor analysis. Therefore, the objective of this work was to validate the use of factor analysis, by means of its estimated gains, for the selection of highly productive and stink bugs resistant genotypes in two soybean segregating populations. For this, the phenotypic evaluation was performed in the generation F2:3, in two distinct experiments, being the populations from the crosses between IAC-100 × PI 295952 and IAC-100 × PI 306712. The experiments were installed in an 18 × 9 alpha-lattice design, with three replicates for each population. Agronomic and resistance characters were evaluated. The factorial scores for each character were obtained for the creation of “supercharacters”. These were designed to check if the selection in the new characters could provide satisfactory simultaneous gains in the original characters. Subsequently, the analysis of variance was performed for all factors, in both populations. The F test showed the presence of variability among genotypes, allowing the selection of superior genotypes. None of the factors selected progenies with all the characters favorably, and their use was not interesting for both populations. With this, complementary studies should be performed with other selection indices in these populations.
DOI: 10.6084/m9.figshare.14276058
title: Budbreak induction in kiwifruit vines cultivated in an organic system by the biological method of single node cutting

description: ABSTRACT Kiwifruit vines are an alternative approach to diversify Brazilian fruit farming because of the low supply and increase in the demand for their fruits. Hydrogenated cyanamide, which is the most common rest-breaking agent, is highly toxic and its use is not allowed in organic production systems. This study aimed at evaluating the efficiency of alternative rest-breaking agents in kiwifruit vines by using the biological method of single node cutting. Twigs of the cultivar Bruno were collected in an organic orchard in Pelotas, a city located in Rio Grande do Sul state, Brazil. Seven-centimeter-long cuts with a single shoot at the ends were segmented. Cuttings were placed on plastic trays with phenolic foam and soaked in water, at 85% relative humidity on average, and kept in bio-oxygen demand (BOD) incubators at 25± 1 °C and a 16-h photoperiod. Budbreak was evaluated in 2-day and 3-day intervals. The experiment was conducted in a completely randomized design with ten treatments and three replications of ten sampling units each. The following variables were analyzed: average budbreak time (ABT), final budbreak rate (FBR), vigorous budbreak rate (VBR), velocity of budbreak (VB), percentage of open buds (POB) and dormancy index (DI). The rest-breaking agent garlic extract (GE) 10% + mineral oil (MO) 2% was the most efficient one in budbreak induction in both production cycles. Thus, this dose is recommended for budbreak induction in kiwifruit vines grown in an organic system.
DOI: 10.6084/m9.figshare.14276052.v1
title: A new proposal for the m + a methodology in segregating populations of cowpea

description: ABSTRACT The evaluation of segregating populations in plant breeding programs is an onerous and time-consuming process. Early identification of populations with genetic potential can be done by m + a methodology. However, the possibility of a modification in the traditional methodology in order to make it more efficient, that is, faster and cheaper, was envisaged. Thus, the objective of this study was to compare the genetic gains obtained by both methodologies, the traditional one and the proposed modification. For this, ten segregating bean-cowpea populations were evaluated at two distinct levels of homozygous F3:4 and F3:5. Genetic values were predicted by two different statistical genetic models. This was possible due to the methodology proposed here to present a much shorter execution time than the traditional methodology. Thus, with a shorter evaluation time, the breeding program manager can plan the evaluation of a larger number of populations in a short time.
DOI: 10.6084/m9.figshare.14276054.v1
title: Role of nonproductive tillers as transient sinks of assimilates in wheat

description: ABSTRACT Although source–sink relationships in wheat are well studied, the performance of tillers as transient sinks is still unclear, especially under stress conditions. Thus, this work aimed to study the yield relationships of wheat stems in contrasting genotypes as affected by tiller treatment under stress conditions. Two experiments were conducted under greenhouse conditions using BRS Guamirim (high tillering) and BRS Parrudo (low tillering) wheat cultivars. Four tillering treatments were applied on late tillers in the first growing season (2016) and three in the second (2017): (i) free-tillering, (ii) detillering, (iii) physical suppression (only in the first season) and (iv) “spikes removal”. The main stem and the first four emitted tillers were considered as primary tillers. Plants were submitted to drought (2016) and defoliation stress (2017) at preanthesis. Intraspecific competition among tillers increased under drought stress conditions and decreased the plant performance. Nonproductive late tillers do not improve the performance of primary tillers of wheat plants under drought stress, mainly in high-tillering cultivars. The reduction on the thousand grain weight of only ‘Parrudo’ detillered plants indicates that assimilate remobilization from late tillers to primary tillers and main stem seems to be more effective in low-tillering cultivars.
DOI: 10.6084/m9.figshare.14276061
title: Rhizobium rhizogenes-mediated transformation of Rhodiola rosea leaf explants

description: ABSTRACT Rhodiola rosea L. is an endangered medicinal plant distributed in mountains and in high latitude regions. For its conservation, sustainable methods for the obtaining of its bioactive compounds must be developed. This work hypothesized that leaf, stem and rhizome explants of R. rosea from different geographical origins respond differently to inoculation with Rhizobium rhizogenes agropine strain ATCC43057. The objective was to generate R. rosea hairy roots (HRs) containing rol-genes. These HRs could be cultivated under axenic conditions for the extraction of the medical compounds rosavinoids and salidroside. Hereby, production of bioactive compounds could be improved per plant biomass. Thirteen R. rosea accessions of Alpine, Scandinavian, Nordic Gene Bank (NGB) and Russian origins were compared for their explant survival and HR formation. Significant differences were observed among plants from different geographical origins, where the NGB leaf explants exhibited up to 70% of HR formation and the Russian accessions did not exhibit HRs at all. Moreover, maintaining explants in light conditions after R. rhizogenes inoculation resulted in higher explant survival and HR formation rate (35%) when compared with explants kept in darkness (9%). Taken together, an efficient HR formation in roseroot by inoculation of R. rhizogenes following culturing in light was reported as a required step. This work represents a stepping-stone to R. rosea HR cultivation in bioreactors as well as regenerating whole plants. Hence, it is initiating a novel route towards high-throughput production of bioactive compounds as well preventing depletion of natural roseroot populations.
DOI: 10.6084/m9.figshare.14276067
title: Selection of Jatropha genotypes for bioenergy purpose: an approach with multitrait, multiharvest and effective population size

description: ABSTRACT Jatropha curcas L. is a perennial plant with great potential for biodiesel production. Thus, the aim of this study was to select J. curcas genotypes for bioenergy purpose considering multitrait, multiharvest and effective population size. To this end, a data set with 70 J. curcas families, obtained through controlled crosses, evaluated in a randomized block design, with six replications, three plants per plot, spaced at 4 × 2 m was used. The following traits were evaluated: plant height in the 2014 and 2015 crop seasons; canopy projection in the row (2015 crop season); canopy projection between rows (2015 crop season); and grain yield (2015, 2016, and 2017 harvests). The mixed model methodology was used to estimate the variance components and to predict the genetic values. Superior genotypes were selected through Multitrait index based on factor analysis and genotype-ideotype distance (FAI-BLUP index), considering the effective population size. According to this index, 98% of all additive genetic variability were summarized in three factors. By adopting the selection intensity that allows maintaining the effective population size equal to 30 (i.e., selection of 214 individuals), the predicted gains were 15.56, 21.75, 4.16, and 5.37% for the traits grain yield in the 2015 and 2016 harvests, canopy projection in the row, and canopy projection between rows in the 2015 crop season, respectively. Thus, the results suggest that the FAI-BLUP index can be successfully used in J. curcas breeding.
DOI: 10.6084/m9.figshare.14276055.v1
title: Greenhouse gas emission, water quality and straw decomposition as a function of rice postharvest field management

description: ABSTRACT The objective of this paper was to evaluate the quality of water drained after the use of the knife-roller (an implement used to incorporate rice residues after harvest), the partial global warming potential (pGWP) and straw decomposition as a result of postharvest field management of irrigated rice. The experiments were conducted during the 2018 and 2019 offseason and the treatments consisted of several water drainage periods (0, 3, 6, 9 and 12 days) after a field pass with a knife-roller. In addition, a nonflooded harvest treatment without straw management was included. Analysis of drainage water three days after a pass with the kniferoller showed a reduction in total soluble solids by 94% compared to zero days. Nitrogen and soluble phosphorus were not influenced by the treatments. However, potassium levels increased as the drainage period increased, which is related to the period between harvest and drainage. As the electrical conductivity is influenced by the concentration of ions, it showed the same response curve as the potassium levels and, for pH, there were small oscillations influenced by the temperature. The total CH4 emission and the pGWP were higher when the water depth remained for a longer period. However, the N2O emissions were higher in the absence of soil submersion. Water should be drained three days after a pass with the knife-roller in order to reduce potassium loss and suspended solids as well as pGWP. The use of the knife-roller accelerated the process of straw decomposition in relation to the unmanaged straw treatment.
DOI: 10.6084/m9.figshare.14276057.v1
title: Selection to high productivity and stink bugs resistance by multivariate data analyses in soybean

description: ABSTRACT Stink bugs that affect soybeans are responsible for significant losses in seed production, quality and germination potential, in addition to hindering the mechanized harvest. To develop insect resistant materials, the breeder can compile a selection index by factor analysis. Therefore, the objective of this work was to validate the use of factor analysis, by means of its estimated gains, for the selection of highly productive and stink bugs resistant genotypes in two soybean segregating populations. For this, the phenotypic evaluation was performed in the generation F2:3, in two distinct experiments, being the populations from the crosses between IAC-100 × PI 295952 and IAC-100 × PI 306712. The experiments were installed in an 18 × 9 alpha-lattice design, with three replicates for each population. Agronomic and resistance characters were evaluated. The factorial scores for each character were obtained for the creation of “supercharacters”. These were designed to check if the selection in the new characters could provide satisfactory simultaneous gains in the original characters. Subsequently, the analysis of variance was performed for all factors, in both populations. The F test showed the presence of variability among genotypes, allowing the selection of superior genotypes. None of the factors selected progenies with all the characters favorably, and their use was not interesting for both populations. With this, complementary studies should be performed with other selection indices in these populations.
DOI: 10.6084/m9.figshare.14276069
title: Effects of entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora on the fitness of a Vip3A resistant subpopulation of Heliothis virescens (Noctuidae: Lepidoptera)

description: ABSTRACT The widespread use of transgenic plants imposes selection pressure on insect pest populations to develop insecticide resistance. Evaluation of effectiveness of resistance management strategies is very important in resistance management programs. Resistance management to insecticides is widely believed to depend in part on associated fitness costs. Fitness costs can delay the development of resistance. In the present study, the effects of two entomopathogenic nematode species, Steinernema carpocapsae and Heterorhabditis bacteriophora were studied on the fitness of first insect population of Heliothis virescens selected with Vip3A in the laboratory. It was found that both nematodes species increased the fitness cost of Vip3A selected insects. The mortality of the Unsel subpopulation after exposure to either nematode species was significantly lower than that of the Vip3A-Sel subpopulation. Likewise, the reproduction of both nematode species was significantly greater in cadavers of the Unsel compared with the Vip3A-Sel subpopulation of H. virescens. There was positive correlation between nematode reproduction and the larval instar infected with nematodes. The penetration of infective nematode juveniles (IJ) was greater in the Vip3A-Sel subpopulation than in the Unsel subpopulation of H. virescens. It is concluded that entomopathogenic nematodes could increase the fitness costs and subsequently delay the resistance.
DOI: 10.6084/m9.figshare.14276058.v1
title: Budbreak induction in kiwifruit vines cultivated in an organic system by the biological method of single node cutting

description: ABSTRACT Kiwifruit vines are an alternative approach to diversify Brazilian fruit farming because of the low supply and increase in the demand for their fruits. Hydrogenated cyanamide, which is the most common rest-breaking agent, is highly toxic and its use is not allowed in organic production systems. This study aimed at evaluating the efficiency of alternative rest-breaking agents in kiwifruit vines by using the biological method of single node cutting. Twigs of the cultivar Bruno were collected in an organic orchard in Pelotas, a city located in Rio Grande do Sul state, Brazil. Seven-centimeter-long cuts with a single shoot at the ends were segmented. Cuttings were placed on plastic trays with phenolic foam and soaked in water, at 85% relative humidity on average, and kept in bio-oxygen demand (BOD) incubators at 25± 1 °C and a 16-h photoperiod. Budbreak was evaluated in 2-day and 3-day intervals. The experiment was conducted in a completely randomized design with ten treatments and three replications of ten sampling units each. The following variables were analyzed: average budbreak time (ABT), final budbreak rate (FBR), vigorous budbreak rate (VBR), velocity of budbreak (VB), percentage of open buds (POB) and dormancy index (DI). The rest-breaking agent garlic extract (GE) 10% + mineral oil (MO) 2% was the most efficient one in budbreak induction in both production cycles. Thus, this dose is recommended for budbreak induction in kiwifruit vines grown in an organic system.
DOI: 10.6084/m9.figshare.14276064
title: May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

description: ABSTRACT Italian ryegrass (Lolium multiflorum Lam.) is a grass weed found in winter cereals, with several glyphosate-resistant diploid populations. Furthermore, the use of Italian ryegrass tetraploid genotypes creates an opportunity to increase the forage demand in replacement of diploid populations. The aim of this research was to evaluate the crossing capacity between glyphosate-resistant diploid and tetraploid Italian ryegrass to transfer glyphosate resistance to the next generation. Reciprocal crosses were performed using the susceptible tetraploid cultivar INIA Titan and resistant diploid (SVA04 biotype) plants to obtain the F1 progeny. Germination testing, survival percentage, inheritance of glyphosate-resistance, and ploidy level were evaluated in F1 progeny. Crossing of susceptible tetraploid plants ♂ and glyphosate-resistant diploid ♀ affected the germination and exhibited 4.5% of triploid plants. F1 progeny from the glyphosate-resistant diploid ♂ and susceptible tetraploid ♀ did not show germination problems, but 46% of the plants died during the initial seedling stage. For this progeny, 10 and 25% of the seedlings were composed of triploid and pentaploid individuals, respectively. Crosses between susceptible tetraploid ♀ and resistant diploid ♂ did not transfer the glyphosate-resistant for the plants. Susceptible tetraploid ♂ crossing with diploid resistant ♀ had a reduction of 47% the glyphosate-resistant plants compared to the homozygous glyphosate-resistant progeny. Overall, tetraploid Italian ryegrass can be a safe tool to reduce and manage the increase of glyphosate-resistant diploid ryegrass in field conditions.
DOI: 10.6084/m9.figshare.14276061.v1
title: Rhizobium rhizogenes-mediated transformation of Rhodiola rosea leaf explants

description: ABSTRACT Rhodiola rosea L. is an endangered medicinal plant distributed in mountains and in high latitude regions. For its conservation, sustainable methods for the obtaining of its bioactive compounds must be developed. This work hypothesized that leaf, stem and rhizome explants of R. rosea from different geographical origins respond differently to inoculation with Rhizobium rhizogenes agropine strain ATCC43057. The objective was to generate R. rosea hairy roots (HRs) containing rol-genes. These HRs could be cultivated under axenic conditions for the extraction of the medical compounds rosavinoids and salidroside. Hereby, production of bioactive compounds could be improved per plant biomass. Thirteen R. rosea accessions of Alpine, Scandinavian, Nordic Gene Bank (NGB) and Russian origins were compared for their explant survival and HR formation. Significant differences were observed among plants from different geographical origins, where the NGB leaf explants exhibited up to 70% of HR formation and the Russian accessions did not exhibit HRs at all. Moreover, maintaining explants in light conditions after R. rhizogenes inoculation resulted in higher explant survival and HR formation rate (35%) when compared with explants kept in darkness (9%). Taken together, an efficient HR formation in roseroot by inoculation of R. rhizogenes following culturing in light was reported as a required step. This work represents a stepping-stone to R. rosea HR cultivation in bioreactors as well as regenerating whole plants. Hence, it is initiating a novel route towards high-throughput production of bioactive compounds as well preventing depletion of natural roseroot populations.