DOI: 10.5061/dryad.kh18932nm
title: Wing geometric morphometry of tabanid flies in Thailand

description: Tabanid flies are gaining high medical and veterinary importance due to
 their role as a vector of many pathogens. In the present study, a total of
 3760 female tabanid flies were collected from Narathiwat and Phayao
 provinces of Thailand. All were identified using the morphological method,
 DNA barcoding, and wing geometric morphometric (WGM) analysis. Eight
 species were identified, and among them, Tabanus tenens is a new recorded
 Thai horse fly. Morphologically, 2178 and 1559 females from Narathiwat and
 Phayao were identified at the species level, including Chrysops dispar,
 Chrysops fasciatus, Tabanus griseilineis, Tabanus rufiscutellatus, and
 Tabanus minimus. The other 23 females were identified at the level of the
 genus (Tabanus spp.) only. Among these, DNA barcoding was further
 identified as Tabanus tenens, Tabanus rubidus, and Tabanus striatus. The
 landmark-based WGM analysis was used to differentiate the samples from
 Narathiwat, and the results showed the efficacy of this approach in
 differentiating the four species of tabanids, achieving an overall
 accuracy score of 99%. Additionally, the data derived from wing landmarks
 of samples collected in Narathiwat were used as reference materials for
 identification of the tabanid fly collected from Phayao, and the finding
 revealed efficacy of the reference materials. Together, this study
 demonstrated that DNA barcoding is a reliable tool for the identification
 of tabanid fly species, while WGM analysis could be a complementary tool.
 The barcode sequences and WGM data generated in this study can serve as a
 valuable reference material to identify new field samples from other
 regions of Thailand. Altogether, this study updated the species list of
 tabanid flies in Thailand, particularly in the Narathiwat and Phayao
 provinces, using various integrative identification tools.
description: # Dryad dataset Dataset DOI:
 [10.5061/dryad.kh18932nm](https://doi.org/10.5061/dryad.kh18932nm) ##
 Description of the data and file structure This dataset contained wing
 pictures of tabanid flies for wing geometric morphometric analysis. For
 characterization and classification, 100 wing pictures were included (25
 pictures for *Chrysops fasciatus*, 25 pictures for *Tabanus
 griseilineis*, 25 pictures for *Tabanus minimus*, and 25 pictures
 for *Tabanus rufiscutellatus*). For identification, 80 wing pictures were
 included (5 pictures for *Chrysops fasciatus*, 25 pictures for *Tabanus
 griseilineis*, 25 pictures for *Tabanus minimus*, and 25 pictures
 for *Tabanus rufiscutellatus*). For repeatability test, landmark
 digitization was repeated twice, 52 pictures were included for analysis
 (13 pictures for *Chrysops fasciatus*, 13 pictures for *Tabanus
 griseilineis*, 13 pictures for *Tabanus minimus*, and 13 pictures
 for *Tabanus rufiscutellatus*). ### Files and variables #### File:
 Wing_pictures_for_landmark_digitization_Characterization_and__Classification.zip **Description:** One hundred wing pictures were included for analysis. #### File: Wing_pictures_for_landmark_digitization_Identification.zip **Description:** Eighty wing pictures were included for analysis. #### File: Wing_pictures_for_repeatability_test_Landmark_digitization_1.zip **Description:** Fifty-two wing pictures were included for analysis. #### File: Wing_pictures_for_repeatability_test_Landmark_digitization_2.zip **Description:** Fifty-two wing pictures were included for analysis. ## Code/software No extra software is needed to view the pictures. Can be viewed with ImageJ.  ## Access information Other publicly accessible locations of the data: * No Data was derived from the following sources: * No