cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/7pwsr-78b43
title: wt_perfusion_fixed_immunofluoro

description: exposure=0.58s, sq6
description: <strong>Tilt Series Date:</strong> 2015-10-26</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -5.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand USC</p>
<strong>Purification / Growth Conditions / Treatment:</strong> This sample is wt cells grown in perfusion set-up. It was imaged on LM for wires, fixed with 4 % formaldehyde for 1 hour, immunofluorescence was done for cytochromes, reimaged, fixed with 2.5% glutaraldehyde for 15 minutes, triple washed with distilled water and saved in distilled water until freezing. The grid was prepared by Sahand on 10/22/15. The grid was frozen on 10/23/15 at Caltech using vitrobot MK4.</p>
<strong>Sample Preparation:</strong> The sample was frozen using vitrobot. C side left. 1.8 ul gold 10nm size was added on opposite side while on vitrobot. conditions: blot force=6, blot time=2.5s, drain time =1s, wait time=0. humidity set 80%. temp 22C. Use 2.5ul gold next time</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-10-26-1/3dimage_70590/</p>wt_immunofluorocyto_sq60001_full.rec,  Reconstruction (Pixel Size 1.28 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-10-26-1/3dimage_70590/wt_immunofluorocyto_sq60001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        wt_immunofluorocyto_sq60001.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-10-26-1/rawdata/wt_immunofluorocyto_sq60001.mrc"
        > <i class="download icon"></i> Download </a></p>

        psa2015-10-26-1_slicer16858.png,  visible nucleoid REZ .  flow lines (nearby strands of DNA tend to be parallel) .  -poorna and andrew, 961.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2015-10-26-1_slicer16858.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/y0b0e-8hm86
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP52sF30 Polara
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3871</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor F30 Polara</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP52 M. smegmatisΔ3871   (ΔEcccA1) sauton media @ 37C</p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-33_slicer6460.jpg: Unknown amw2013-06-10-33_slicer6461.jpg: Unknown amw2013-06-10-33_slicer6462.jpg: Unknown amw2013-06-10-33_slicer7462.jpg: filaments
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-33/rawdata/</p>kp52s0010.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-33/rawdata/kp52s0010.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp52s0010_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-33/3dimage_27601/kp52s0010_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w4msc-rcg27
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP48
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3877</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP48 M. smegmatis Δ3877 (ΔeccD1) 7H9 media </p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-13_slicer8559.jpg: Unknown twisted filament bundle .  Mycobacter smegmatis
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-13/rawdata/</p>kp48_10011.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-13/rawdata/kp48_10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp48_10011_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-13/3dimage_27550/kp48_10011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.zdm83z
title: Occurrence Download

description: A dataset containing 38 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "PublishingCountry is Colombia",
 {
 "and" : [
 "Country is Colombia",
 {
 "not" : {
 "Issue" : [
 "is Country coordinate mismatch"
 ]
 }
 }
 ]
 }
 ]
 },
 {
 "and" : [
 "TaxonKey is one of (Paulosawaya [GBIF Backbone Taxonomy])",
 "PublishingOrg is one of (2a7e3080-28a9-11dd-97cd-b8a03c50a862)"
 ]
 }
 ]
}

 The dataset includes 38 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047924-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/r2zxp-f9w09
title: Hyphomonas session15 27.5KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-06-05</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, chloroquine 0.1mg/ml...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
  jsa2009-06-05-7_slicer9285.jpg: Hyphomonas neptunium   .  DNA ? .  Filament jsa2009-06-05-7_slicer9286.jpg: Hyphomonas neptunium  .  Tube jsa2009-06-05-7_slicer9287.jpg: Hyphomonas neptunium  .  Tube
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-05-7/rawdata/</p>09jun05a_bx467b_037.mrc,  Tilt Series (Pixel Size 0.774 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-05-7/rawdata/09jun05a_bx467b_037.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jun05a_bx467b_037_part.rec,  Reconstruction (Pixel Size 1.548 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-06-05-7/3dimage_7720/09jun05a_bx467b_037_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4z7e3-sws97
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP52s
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3871</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP52 M. smegmatisΔ3871   (ΔEcccA1) sauton media @ 37C</p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-29_slicer6906.jpg: Pointy vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-29/rawdata/</p>kp52s0006.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-29/rawdata/kp52s0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp52s0006_part121_1.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-29/3dimage_27585/kp52s0006_part121_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tq8e0-3qy56
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP48
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3877</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP48 M. smegmatis Δ3877 (ΔeccD1) 7H9 media </p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-18_slicer7422.jpg: Filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-18/rawdata/</p>kp48_10016.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-18/rawdata/kp48_10016.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp48_10016_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-18/3dimage_27560/kp48_10016_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.cpdtjw
title: Occurrence Download

description: A dataset containing 262 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Argyranthemum frutescens",
 "Country is Australia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 262 records from 22 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047927-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.kfx3j6
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Perdicula asiatica",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047925-250811113504898/datasets/export for details.
DOI: 10.22002/pe9aw-kv586
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP52s
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3871</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP52 M. smegmatisΔ3871   (ΔEcccA1) sauton media @ 37C</p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-38_slicer10435.jpg: Micobacterium smegmatis .  vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-38/rawdata/</p>kp52s0015.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-38/rawdata/kp52s0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp52s0015_part121_3.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-38/3dimage_27599/kp52s0015_part121_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/241s6-dej24
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP48
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3877</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP48 M. smegmatis Δ3877 (ΔeccD1) 7H9 media </p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-15_slicer7419.jpg: REZ
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-15/rawdata/</p>kp48_10013.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-15/rawdata/kp48_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp48_10013_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-15/3dimage_27554/kp48_10013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ya0yz-t7034
title: Mycobacter smegmatis pneumonia

description: type VII secretion system in Mycobacterium smegmatis Wild type Mycobacterium smegmatis strain is expected to have T7SS, while mutant to be defective.KP48
description: <strong>Tilt Series Date:</strong> 2013-06-10</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> 3877</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul /  Eric BrownGenentech, Inc.1 DNA WaySouth San Francisco, CA 94080(650) 225-6248Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> KP48 M. smegmatis Δ3877 (ΔeccD1) 7H9 media </p>
<strong>Sample Preparation:</strong> 3ul + 10nm AuR2/2 quantifoils 4.5offset 1 blot 2s 1s drain</p>
  amw2013-06-10-19_slicer7423.jpg: Filaments  .  Filament bundle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-19/rawdata/</p>kp48_10017.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-19/rawdata/kp48_10017.mrc"
        > <i class="download icon"></i> Download </a></p>

        kp48_10017_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-06-10-19/3dimage_27562/kp48_10017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.vw42wm
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Argyranthemum frutescens",
 "Country is Armenia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047926-250811113504898/datasets/export for details.
DOI: 10.22002/yacjt-zh610
title: JV9298

description: Legionella pneumophila JV9298 DotF-GFP DotB E191K
description: <strong>Tilt Series Date:</strong> 2020-04-23</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O.D. 2.5-3.0 in AYE</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-119/3dimage_123918/</p>JV9298_PD-1-3_488_SIRT_1k.mrc,  Reconstruction (Pixel Size 1.04 nm), 2.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-04-23-119/3dimage_123918/JV9298_PD-1-3_488_SIRT_1k.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9298_PD-1-3_488.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.7 GB  
        
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DOI: 10.15468/dl.4vdyvj
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Argyranthemum frutescens",
 "Country is Belgium",
 "HasCoordinate is true"
 ]
}

 The dataset includes 5 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047928-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.