cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20025949
title: Potential dispersal of aquatic snails by waterbird endozoochory in neotropical wetlands

description: Abstract Waterbird-mediated zoochory is one of the main ecological mechanisms by which non-flying freshwater invertebrates can disperse between isolated wetlands. Passive dispersal through gut passage inside waterbirds (endozoochory) may explain how many organisms spread in the landscape. Here, we evaluate the potential for dispersal of aquatic snails by three waterbird species in neotropical wetlands. A total of 77 faecal samples from Coscoroba coscoroba (n = 28), Dendrocygna viduata (n = 36) and Anas flavirostris (n = 13) were collected in the field and taken to the laboratory. There, the samples were examined under a stereomicroscope to check for the presence of gastropod shells. We found 496 intact gastropod shells, and Heleobia piscium was the most abundant species (n= 485). We also found two shells of Drepanotrema sp. and nine others distributed between two different morphotypes of Planorbidae. Snails were present in 20.8 % of all samples, and were more frequent in faeces of coscoroba swan (50%) than the other two bird species. Our data suggest that aquatic snails may disperse by avian endozoochory between neotropical wetlands, with vectors including migratory bird species.
DOI: 10.6084/m9.figshare.20025949.v1
title: Potential dispersal of aquatic snails by waterbird endozoochory in neotropical wetlands

description: Abstract Waterbird-mediated zoochory is one of the main ecological mechanisms by which non-flying freshwater invertebrates can disperse between isolated wetlands. Passive dispersal through gut passage inside waterbirds (endozoochory) may explain how many organisms spread in the landscape. Here, we evaluate the potential for dispersal of aquatic snails by three waterbird species in neotropical wetlands. A total of 77 faecal samples from Coscoroba coscoroba (n = 28), Dendrocygna viduata (n = 36) and Anas flavirostris (n = 13) were collected in the field and taken to the laboratory. There, the samples were examined under a stereomicroscope to check for the presence of gastropod shells. We found 496 intact gastropod shells, and Heleobia piscium was the most abundant species (n= 485). We also found two shells of Drepanotrema sp. and nine others distributed between two different morphotypes of Planorbidae. Snails were present in 20.8 % of all samples, and were more frequent in faeces of coscoroba swan (50%) than the other two bird species. Our data suggest that aquatic snails may disperse by avian endozoochory between neotropical wetlands, with vectors including migratory bird species.
DOI: 10.6084/m9.figshare.20025951
title: Emergence of polymyxin B-resistant Acinetobacter baumannii in hospitals in Rio de Janeiro

description: ABSTRACT Introduction: Acinetobacter baumannii has been considered a prevalent pathogen in hospitals, raising concern in the medical community due to its broad spectrum of antimicrobial resistance. Since it is a subject that arouses much interest, it has been increasingly studied. Due to the emergence of multidrug-resistant (MDR) Gram-negative bacteria, the use of polymyxins was reestablished. The polymyxins have been considered the only option for the treatment of severe infections caused by MDR A. baumannii. Objective: To investigate the susceptibility profile of A. baumannii to polymyxin B. Material and method: 92 clinical isolates from two public hospitals in the Rio de Janeiro city were studied using broth microdilution method. Results: Most of the isolates were resistant to polymyxin B, 81.5% (n = 75), and minimum inhibitory concentration (MIC) values ranged between 4-64 mcg/ml. Conclusion: These results are a concern since currently the polymyxins have been considered the most effective therapeutic option against MDR isolates of A. baumannii.
DOI: 10.6084/m9.figshare.20025951.v1
title: Emergence of polymyxin B-resistant Acinetobacter baumannii in hospitals in Rio de Janeiro

description: ABSTRACT Introduction: Acinetobacter baumannii has been considered a prevalent pathogen in hospitals, raising concern in the medical community due to its broad spectrum of antimicrobial resistance. Since it is a subject that arouses much interest, it has been increasingly studied. Due to the emergence of multidrug-resistant (MDR) Gram-negative bacteria, the use of polymyxins was reestablished. The polymyxins have been considered the only option for the treatment of severe infections caused by MDR A. baumannii. Objective: To investigate the susceptibility profile of A. baumannii to polymyxin B. Material and method: 92 clinical isolates from two public hospitals in the Rio de Janeiro city were studied using broth microdilution method. Results: Most of the isolates were resistant to polymyxin B, 81.5% (n = 75), and minimum inhibitory concentration (MIC) values ranged between 4-64 mcg/ml. Conclusion: These results are a concern since currently the polymyxins have been considered the most effective therapeutic option against MDR isolates of A. baumannii.
DOI: 10.6084/m9.figshare.20025966
title: Features of the female prostate according to age: an autopsy study

description: ABSTRACT INTRODUCTION: The prostate gland plays an important role in male and female reproductive system. Data on this organ have not been fully explored in women since its first description, probably because it is considered a vestigial gland. OBJECTIVE: To correlate the morphology of the female prostate with age in autopsy. MATERIAL AND METHODS: Thirty-two female cadavers, 31 adults and one newborn, underwent dissection of the region corresponding to the prostate for histological analysis. The urethral region was divided into three portions: proximal, median, and distal. All the glands present in the samples were counted. Clinical data were collected, including age and previous diagnosis of menopause. RESULTS: There were no macroscopically visible prostate. Morphological analyses showed glands surrounding the urethra with a stratified epithelium, ranging from squamous to columnar types, with prevalence of basophilic cells and some presenting with secretion inside. A significant correlation with prostate tissue was found between the median and the proximal urethra, as well as between the median and distal urethra, suggesting that when the glandular structures increase in the median region, there is also an increase in the anterior and distal structures. Moreover, a prevalence of the glands in the median urethra was observed in post-menopausal women. CONCLUSION: This study suggests that the number of female prostate glands increases after menopause, with proliferative spread and growth of the median portion to the proximal and distal portions.
DOI: 10.6084/m9.figshare.20025966.v1
title: Features of the female prostate according to age: an autopsy study

description: ABSTRACT INTRODUCTION: The prostate gland plays an important role in male and female reproductive system. Data on this organ have not been fully explored in women since its first description, probably because it is considered a vestigial gland. OBJECTIVE: To correlate the morphology of the female prostate with age in autopsy. MATERIAL AND METHODS: Thirty-two female cadavers, 31 adults and one newborn, underwent dissection of the region corresponding to the prostate for histological analysis. The urethral region was divided into three portions: proximal, median, and distal. All the glands present in the samples were counted. Clinical data were collected, including age and previous diagnosis of menopause. RESULTS: There were no macroscopically visible prostate. Morphological analyses showed glands surrounding the urethra with a stratified epithelium, ranging from squamous to columnar types, with prevalence of basophilic cells and some presenting with secretion inside. A significant correlation with prostate tissue was found between the median and the proximal urethra, as well as between the median and distal urethra, suggesting that when the glandular structures increase in the median region, there is also an increase in the anterior and distal structures. Moreover, a prevalence of the glands in the median urethra was observed in post-menopausal women. CONCLUSION: This study suggests that the number of female prostate glands increases after menopause, with proliferative spread and growth of the median portion to the proximal and distal portions.
DOI: 10.6084/m9.figshare.20025967
title: Kinetics of the expressions of radiation-induced plasma proteins of the cardiac territory in electrophoresis

description: ABSTRACT Introduction: Radiation induces acute and late alterations in blood proteins. Objective: The present study aims at analyzing in time kinetics the electrophoretic profiles of expression bands of blood protein, in the molecular weight range greater than and equal to that of albumin, modulated by ionizing radiation in the cardiac territory, in animal model. Material and methods: Animals were exposed to a whole-body dose of 5 Gy ionizing radiation (Co-60). Serum samples were collected from isogenic Wistar rats (control and irradiated groups). At a time kinetics of 12, 24, 48, 72, 96 hours and 35 days post-irradiation, thoracolaparotomies were performed with anesthesia, and 0.3 ml of blood was collected between the left ventricle and the pulmonary artery. The samples were held in heparin and their components were separated by centrifugation. Protein bands with similar molecular weight were identified by vertical 10% electrophoresis, silver staining. Results: The findings indicate a systemic acute altered expression of proteins with molecular weight greater than or equal to that of albumin in acrylamide gel, presenting suppression and increased expression due to modulation of preexisting bands, identified in time kinetics. Conclusion: These findings point out to acute alterations of protein expression modulated in time, but also to a late modulation of gene expression.
DOI: 10.6084/m9.figshare.20025967.v1
title: Kinetics of the expressions of radiation-induced plasma proteins of the cardiac territory in electrophoresis

description: ABSTRACT Introduction: Radiation induces acute and late alterations in blood proteins. Objective: The present study aims at analyzing in time kinetics the electrophoretic profiles of expression bands of blood protein, in the molecular weight range greater than and equal to that of albumin, modulated by ionizing radiation in the cardiac territory, in animal model. Material and methods: Animals were exposed to a whole-body dose of 5 Gy ionizing radiation (Co-60). Serum samples were collected from isogenic Wistar rats (control and irradiated groups). At a time kinetics of 12, 24, 48, 72, 96 hours and 35 days post-irradiation, thoracolaparotomies were performed with anesthesia, and 0.3 ml of blood was collected between the left ventricle and the pulmonary artery. The samples were held in heparin and their components were separated by centrifugation. Protein bands with similar molecular weight were identified by vertical 10% electrophoresis, silver staining. Results: The findings indicate a systemic acute altered expression of proteins with molecular weight greater than or equal to that of albumin in acrylamide gel, presenting suppression and increased expression due to modulation of preexisting bands, identified in time kinetics. Conclusion: These findings point out to acute alterations of protein expression modulated in time, but also to a late modulation of gene expression.
DOI: 10.6084/m9.figshare.20025970
title: Detection of antimicrobial resistance genes in beta-lactamase- and carbapenemase-producing Klebsiella pneumoniae by patient surveillance cultures at an intensive care unit in Rio de Janeiro, Brazil

description: ABSTRACT Introduction: The increasing incidence of multi-resistant microorganisms has been considered a public health problem. One of the routines included in hospital practice is the screening of colonized and/or infected patients. Objective: The aim of this study was to evaluate the genetic variability and clonal relationships of extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae, from surveillance cultures, at an intensive care unit, in Rio de Janeiro, Brazil. Material and methods: Seventy K. pneumoniae isolates were obtained from rectal swabs (March 2013 to March 2014). Antimicrobial susceptibility was assessed by VITEK 2 System. Resistant genes blaSHV, blaTEM, blaOXA-1, blaKPC, blaOXA-48, blaCTX-M-15, blaVIM, blaIMP and blaNDM were investigated by polymerase chain reaction (PCR); genetic diversity, by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). Results: Strains showed high resistance rates to cefepime (94%), ceftazidime (96%), ertapenem (61%), imipenem (54%) meropenem (43%) and ciprofloxacin (69%). The most prevalent genes were blaSHV (69%), blaTEM (63%), blaOXA-1 (60%), blaKPC (57%), blaCTX-M-15 (47%), blaOXA-48 (16%). Genes blaVIM, blaIMP and blaNDM were not detected. Twenty nine profiles of resistance genes were observed, with 23% carrying at least five genes. A great genetic diversity (68 ERIC profiles) was also observed among the strains. Conclusion: Although no clonal relationship was observed within the isolates, this study revealed alarming data on the antimicrobial resistance deficiently monitored for preventive purposes in Brazil. Our data allow us to conclude that the inclusion of surveillance cultures in health facilities is a recommended strategy aiming particularly at preventing the spread of resistance genes in the hospital environment and, consequently, reducing morbidity and mortality.
DOI: 10.6084/m9.figshare.20025970.v1
title: Detection of antimicrobial resistance genes in beta-lactamase- and carbapenemase-producing Klebsiella pneumoniae by patient surveillance cultures at an intensive care unit in Rio de Janeiro, Brazil

description: ABSTRACT Introduction: The increasing incidence of multi-resistant microorganisms has been considered a public health problem. One of the routines included in hospital practice is the screening of colonized and/or infected patients. Objective: The aim of this study was to evaluate the genetic variability and clonal relationships of extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae, from surveillance cultures, at an intensive care unit, in Rio de Janeiro, Brazil. Material and methods: Seventy K. pneumoniae isolates were obtained from rectal swabs (March 2013 to March 2014). Antimicrobial susceptibility was assessed by VITEK 2 System. Resistant genes blaSHV, blaTEM, blaOXA-1, blaKPC, blaOXA-48, blaCTX-M-15, blaVIM, blaIMP and blaNDM were investigated by polymerase chain reaction (PCR); genetic diversity, by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). Results: Strains showed high resistance rates to cefepime (94%), ceftazidime (96%), ertapenem (61%), imipenem (54%) meropenem (43%) and ciprofloxacin (69%). The most prevalent genes were blaSHV (69%), blaTEM (63%), blaOXA-1 (60%), blaKPC (57%), blaCTX-M-15 (47%), blaOXA-48 (16%). Genes blaVIM, blaIMP and blaNDM were not detected. Twenty nine profiles of resistance genes were observed, with 23% carrying at least five genes. A great genetic diversity (68 ERIC profiles) was also observed among the strains. Conclusion: Although no clonal relationship was observed within the isolates, this study revealed alarming data on the antimicrobial resistance deficiently monitored for preventive purposes in Brazil. Our data allow us to conclude that the inclusion of surveillance cultures in health facilities is a recommended strategy aiming particularly at preventing the spread of resistance genes in the hospital environment and, consequently, reducing morbidity and mortality.
DOI: 10.6084/m9.figshare.20025972
title: Use of the fluid obtained by puncture of cystic hygroma: an alternative method for fetal karyotyping

description: ABSTRACT The aim of our study aim was to report the case of a fetus with Turner syndrome (TS) diagnosed by karyotype from cystic hygroma (CH) fluid, highlighting the applications and importance of this procedure. First-trimester screening revealed an increased nuchal translucency measurement, cervical cystic hygroma and head and trunk subcutaneous edema. The presence of oligohydramnios prevented the performance of amniocentesis. We performed puncture of the CH for fetal karyotyping, which revealed X-chromosome monosomy (45,X), compatible with TS. Therefore, the use of CH fluid as an alternative sample for fetal karyotyping may be considered when conventional invasive procedures can not be performed.
DOI: 10.6084/m9.figshare.20025972.v1
title: Use of the fluid obtained by puncture of cystic hygroma: an alternative method for fetal karyotyping

description: ABSTRACT The aim of our study aim was to report the case of a fetus with Turner syndrome (TS) diagnosed by karyotype from cystic hygroma (CH) fluid, highlighting the applications and importance of this procedure. First-trimester screening revealed an increased nuchal translucency measurement, cervical cystic hygroma and head and trunk subcutaneous edema. The presence of oligohydramnios prevented the performance of amniocentesis. We performed puncture of the CH for fetal karyotyping, which revealed X-chromosome monosomy (45,X), compatible with TS. Therefore, the use of CH fluid as an alternative sample for fetal karyotyping may be considered when conventional invasive procedures can not be performed.
DOI: 10.6084/m9.figshare.20025973.v1
title: Daily intake of watercress causes inhibition of experimental Ehrlich tumor growth

description: ABSTRACT Introduction: Increasing cruciferous vegetable intake has been associated with reduced risk of cancer. Experimental and epidemiological studies suggest that the watercress it is a cruciferous vegetable with high concentration of compounds with recognized antitumor activity. Objective: To investigate the effects of daily intake of an aqueous solution of watercress on the growth of the experimental Ehrlich tumor (EET). Methods: Swiss mice were divided into three groups A, B and C (n = 6). The animals from the control group (A) received, by gavage, 0.05 ml of saline throughout the experiment. The animals in group B, from the first day of the experiment, received daily, by gavage, 0.05 ml of watercress aqueous solution (0.5 g/ml). The animals from group C began, on day 21, daily intake of this solution. At day 21.2 × 106 EET cells were inoculated subcutaneously in the left footpad of all mice, and tumor growth was assessed by measuring the thickness of the paw. On day 42, the animals were sacrificed and their footpads removed for histopathological analysis. Results: The animals from groups B and C have showed a suppression of tumor growth and a small area of necrosis compared to the animals of group A. Conclusion: The present study demonstrated that the daily intake of aqueous solution of watercress was able to activate a suppression of the EET growth, probably due to the main compounds with antitumor properties present in this vegetable.
DOI: 10.6084/m9.figshare.20025973
title: Daily intake of watercress causes inhibition of experimental Ehrlich tumor growth

description: ABSTRACT Introduction: Increasing cruciferous vegetable intake has been associated with reduced risk of cancer. Experimental and epidemiological studies suggest that the watercress it is a cruciferous vegetable with high concentration of compounds with recognized antitumor activity. Objective: To investigate the effects of daily intake of an aqueous solution of watercress on the growth of the experimental Ehrlich tumor (EET). Methods: Swiss mice were divided into three groups A, B and C (n = 6). The animals from the control group (A) received, by gavage, 0.05 ml of saline throughout the experiment. The animals in group B, from the first day of the experiment, received daily, by gavage, 0.05 ml of watercress aqueous solution (0.5 g/ml). The animals from group C began, on day 21, daily intake of this solution. At day 21.2 × 106 EET cells were inoculated subcutaneously in the left footpad of all mice, and tumor growth was assessed by measuring the thickness of the paw. On day 42, the animals were sacrificed and their footpads removed for histopathological analysis. Results: The animals from groups B and C have showed a suppression of tumor growth and a small area of necrosis compared to the animals of group A. Conclusion: The present study demonstrated that the daily intake of aqueous solution of watercress was able to activate a suppression of the EET growth, probably due to the main compounds with antitumor properties present in this vegetable.
DOI: 10.6084/m9.figshare.5720848
title: Primary cardiac angiosarcoma: case report of a rare neoplasia

description: ABSTRACT This article reports a case of primary cardiac angiosarcoma and a brief review is provided. A 44-year-old male patient was suspected of having myxoma in the right atrium. The tumor, on pathology examination, was shown to be a cardiac angiosarcoma. In the postoperative period, the patient developed a cardiac tamponade, requiring reoperation and evolving to death. Angiosarcomas are malignant tumors characterized by a devastating clinical course. They have a predilection for the right atrium, occurring between the third and fifth decades of life, with a male preponderance. Because of its rarity, the ideal treatment has not been identified yet.
DOI: 10.6084/m9.figshare.5720782
title: Pancreatic adenosquamous carcinoma, a rare entity: report of four cases

description: ABSTRACT Adenosquamous carcinoma of the pancreas (ASCP) is a rare variant of the pancreatic ductal adenocarcinoma (PDAC). Between 2004 and 2016, four cases of ASCP were resected at our institution; clinicopathological data were collected. All of our patients were males, aged 55-80 years. Three cases were cephalic tumors; and one, pancreatic tail tumor, measuring between 2.3 and 5.5 cm. All had neurovascular invasion and lymphatic metastasis. Two had retroperitoneal positive margins. The overall survival (OS) after surgery was three weeks-42 months. Prognosis of ASCP is dark and OS appears to be more closely related to surgical margins status than to other clinicopathological factors.
DOI: 10.6084/m9.figshare.5720848.v1
title: Primary cardiac angiosarcoma: case report of a rare neoplasia

description: ABSTRACT This article reports a case of primary cardiac angiosarcoma and a brief review is provided. A 44-year-old male patient was suspected of having myxoma in the right atrium. The tumor, on pathology examination, was shown to be a cardiac angiosarcoma. In the postoperative period, the patient developed a cardiac tamponade, requiring reoperation and evolving to death. Angiosarcomas are malignant tumors characterized by a devastating clinical course. They have a predilection for the right atrium, occurring between the third and fifth decades of life, with a male preponderance. Because of its rarity, the ideal treatment has not been identified yet.
DOI: 10.6084/m9.figshare.5720782.v1
title: Pancreatic adenosquamous carcinoma, a rare entity: report of four cases

description: ABSTRACT Adenosquamous carcinoma of the pancreas (ASCP) is a rare variant of the pancreatic ductal adenocarcinoma (PDAC). Between 2004 and 2016, four cases of ASCP were resected at our institution; clinicopathological data were collected. All of our patients were males, aged 55-80 years. Three cases were cephalic tumors; and one, pancreatic tail tumor, measuring between 2.3 and 5.5 cm. All had neurovascular invasion and lymphatic metastasis. Two had retroperitoneal positive margins. The overall survival (OS) after surgery was three weeks-42 months. Prognosis of ASCP is dark and OS appears to be more closely related to surgical margins status than to other clinicopathological factors.