cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/k7fej-amr43
title: Treponema primitia Proteinase K

description: Raw data files of Treponema primitia Proteinase K
description: <strong>Tilt Series Date:</strong> 2012-04-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Treponema primitia</p>
<strong>Strain:</strong> ZAS-2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in the presence of proteinase K for 15min</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-27-1/rawdata/</p>protk0001.mrc,  Tilt Series (Pixel Size 1.9 nm), 842.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-27-1/rawdata/protk0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        protk0001_full.rec,  Reconstruction (Pixel Size 3.8 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-04-27-1/3dimage_18298/protk0001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        etl2012-04-27-1_slicer19174.png,  ? .  -andrew, 787.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/etl2012-04-27-1_slicer19174.png"
        > <i class="download icon"></i> Download </a></p>

        etl2012-04-27-1_slicer19175.png,  775.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/etl2012-04-27-1_slicer19175.png"
        > <i class="download icon"></i> Download </a></p>

        etl2012-04-27-1_slicer19176.png,  427.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/etl2012-04-27-1_slicer19176.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nxc0g-4ns94
title: Xanthomonas vs Caulobacter

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter competition
description: <strong>Tilt Series Date:</strong> 2017-03-30</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the strains, data collected by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-30-97/rawdata/</p>Xac_Cc_20170329b0001.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-30-97/rawdata/Xac_Cc_20170329b0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_20170329b0001_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-30-97/3dimage_109206/Xac_Cc_20170329b0001_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_20170329b0001.ali,  270.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-30-97/file_26075/Xac_Cc_20170329b0001.ali"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_20170329b0001.tlt,  304.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-30-97/file_26076/Xac_Cc_20170329b0001.tlt"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_20170329b0001_fid.tlt,  304.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-30-97/file_26077/Xac_Cc_20170329b0001_fid.tlt"
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DOI: 10.22002/89jn1-zn171
title: Xanthomonas vs Caulobacter

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter competition
description: <strong>Tilt Series Date:</strong> 2017-03-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the strains, data collected by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 sec</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-29-5/rawdata/</p>Xac_Cc_201703292nda0003.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-29-5/rawdata/Xac_Cc_201703292nda0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_201703292nda0003_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-29-5/3dimage_109174/Xac_Cc_201703292nda0003_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_201703292nda0003.ali,  491.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-29-5/file_25948/Xac_Cc_201703292nda0003.ali"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_201703292nda0003.tlt,  552.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-29-5/file_25949/Xac_Cc_201703292nda0003.tlt"
        > <i class="download icon"></i> Download </a></p>

        Xac_Cc_201703292nda0003_fid.tlt,  552.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-29-5/file_25950/Xac_Cc_201703292nda0003_fid.tlt"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/35jah-dhp42
title: Xanthomonas vs Caulobac (-T1SS)

description: Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter (delta-cdzAB) competition. Caulobacter strain is a T1SS deletion strain.
description: <strong>Tilt Series Date:</strong> 2017-10-27</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Xanthomonas citri</p>
<strong>Strain:</strong> pv citri 306</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> TOMO3D</p>
<strong>Collaborators and Roles:</strong> Chuck Farah lab made the Xac strain, Caulobacter strain is from Laub lab. Data collected and processed by Mohammed Kaplan and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.</p>
<strong>Sample Preparation:</strong> Manual blotting, humidity 100%</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-27-17/rawdata/</p>Xan_CaulDelT1_20172710g0005.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-27-17/rawdata/Xan_CaulDelT1_20172710g0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172710g0005_SIRT_1k.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-27-17/3dimage_109105/Xan_CaulDelT1_20172710g0005_SIRT_1k.rec"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172710g0005.ali,  370.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-27-17/file_25739/Xan_CaulDelT1_20172710g0005.ali"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172710g0005.tlt,  416.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-27-17/file_25741/Xan_CaulDelT1_20172710g0005.tlt"
        > <i class="download icon"></i> Download </a></p>

        Xan_CaulDelT1_20172710g0005_fid.tlt,  416.0 B  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-27-17/file_25744/Xan_CaulDelT1_20172710g0005_fid.tlt"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ywaae-k9d15
title: Rhesus TRIM5α decorated HIV-1

description: Non infectious hyperstable HIV-1 cores decorated with Rhesus TRIM5α. 0.3 µM sulfo-EGS. Cores were heavily coated.
description: <strong>Tilt Series Date:</strong> 2015-06-14</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -5.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Wes Sunquist, Barbie Ganser-Pornillos</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Yen-Li (Wes Sundquist Lab)</p>
<strong>Sample Preparation:</strong> Quantifoil 300 mesh R2/2</p>
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-7/rawdata/</p>tomographycores0007.mrc,  Tilt Series (Pixel Size 2.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-7/rawdata/tomographycores0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomographycores0007_full.rec,  Reconstruction (Pixel Size 0.0 nm), 444.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-7/3dimage_76044/tomographycores0007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomographycores0007_fullclip.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 11.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-7/3dimage_76045/tomographycores0007_fullclip.sirt.rec"
        > <i class="download icon"></i> Download </a></p>

        tomographycores0007_fullclipnad.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 11.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-06-14-7/3dimage_76046/tomographycores0007_fullclipnad.sirt.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9m36y-9vd27
title: #92+E.coli bamA purified

description: Raw data files of #92+E.coli bamA purified
description: <strong>Tilt Series Date:</strong> 2015-04-29</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chris Hayes and David Low UCSB</p>
<strong>Sample Preparation:</strong> These are E.cloacae mini cells (#92) mixed with purified E.coli BamA protein receptor. Both cells and BamA were blocked with BSA- 100g/ml for 30 minutes before mixing for 20 minutes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-3/rawdata/</p>PS96A-tomo0016.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-3/rawdata/PS96A-tomo0016.mrc"
        > <i class="download icon"></i> Download </a></p>

        PS96A-tomo0016_full.rec,  Reconstruction (Pixel Size 1.32 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-3/3dimage_55157/PS96A-tomo0016_full.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2015-04-29-3_slicer19371.png, , 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2015-04-29-3_slicer19371.png"
        > <i class="download icon"></i> Download </a></p>

        psa2015-04-29-3_slicer19372.png, , 1.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2015-04-29-3_slicer19372.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z7gkz-jvh80
title: Cell line25 incubated MG-132

description: 4 hours incubation MG-132 (0.4 ul) 1 μg/ml. Added 500 ul of Vpr-tomato pseudovirus to each well.
description: <strong>Tilt Series Date:</strong> 2016-02-29</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Tom Hope</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown on R2/2 London finder grids coated with fibronectin</p>
<strong>Sample Preparation:</strong> Manual blotting</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-3/rawdata/</p>tomography3.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-3/rawdata/tomography3.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography3_part121_7.rec,  Reconstruction (Pixel Size 2.4 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-3/3dimage_91253/tomography3_part121_7.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography3_orignew_full.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-3/3dimage_91460/tomography3_orignew_full.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography3_orignew_full1.rec,  Reconstruction (Pixel Size 0.0 nm), 668.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2016-02-29-3/3dimage_91461/tomography3_orignew_full1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mbg68-9r983
title: Crosslinked HIV-1 cores TRIM5α

description: Hyperstable purified HIV-1 cores crosslinked with TRIM5α
description: <strong>Tilt Series Date:</strong> 2015-01-03</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> HIV cores</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 109.0 eV/Å²,  defocus: -5.0 μm,  magnification: 41000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grids frozen in Utah</p>
<strong>Sample Preparation:</strong> Non infectious HIV-1 cores decorated with TRIM5α. 0.3 µM sulfo-EGS</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-13/3dimage_73490/</p>tomography0014.rec,  Reconstruction (Pixel Size 0.0 nm), 398.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-13/3dimage_73490/tomography0014.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography14.nad.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 5.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-13/3dimage_73491/tomography14.nad.sirt.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography14.sirt.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-13/3dimage_73492/tomography14.sirt.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography0014.mrc,  Tilt Series (Pixel Size 0.26 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-01-03-13/rawdata/tomography0014.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x7xv8-qkv89
title: #92+E.coli bamA purified

description: Raw data files of #92+E.coli bamA purified
description: <strong>Tilt Series Date:</strong> 2015-04-29</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chris Hayes and David Low UCSB</p>
<strong>Sample Preparation:</strong> These are E.cloacae mini cells (#92) mixed with purified E.coli BamA protein receptor. Both cells and BamA were blocked with BSA- 100g/ml for 30 minutes before mixing for 20 minutes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-29-18/rawdata/</p>PS96A-tomo0020.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.8 GB  
        
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        PS96A-tomo0020_part67_1.rec,  Reconstruction (Pixel Size 1.32 nm), 430.6 MB  
        
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        psa2015-04-29-18_slicer19373.png, , 1.3 MB  
        
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        psa2015-04-29-18_slicer19374.png, , 1.3 MB  
        
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