cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/cu.the.2019.112
title: Genetic characteristics of avian influenza a viruses isolated from Thai free-grazing ducks and their pathogenicity potential in animal models

description: Influenza A viruses (IAVs) can infect several animal species and humans. In Thailand, free-grazing ducks are reservoir hosts of influenza A virus. The free-grazing ducks are potentially interface with wild birds and domestic birds and subsequently cause interspecies transmission of IAVs. This thesis composes of three study phases. Phase 1 is surveillance of influenza A in free-grazing ducks. Phase 2 is genetic characterization and diversity analysis of influenza A viruses from free-grazing ducks. Phase 3 is challenge of influenza A viruses isolated from free-grazing ducks in animal models. For phase 1, multi-years surveillance of IAVs in free-grazing ducks was conducted in central and lower northern parts of Thailand. Our results found 1.77% of influenza A virus from oropharyngeal swabs (n=2,012) and cloacal swabs (n=2,012), 70.24% of influenza A antibody and 6.40% of specific H5 subtype antibody from serum samples (n=1,959). Of 71 IAV positive samples, 15 subtypes of IAVs could be identified and the predominant subtypes were subtypes H3N8 and H4N6. For phase 2, representative viruses (n=26) were characterized by whole genome sequencing. The results from phylogenetic analysis of 8 genes of Thai influenza viruses revealed new reassortant viruses (n=3) (H11N9, H4N6 and H11N7). While the origin of most Thai IAVs was from Avian Eurasian lineage I. According to the origins of each gene, the gene constellation of 26 representative viruses was identified to 24 patterns. For phase 3, influenza A virus subtype H1N3 (n=1) and H11N9 (n=1) were selected for animal challenge study. Both H1N3 and H11N9 viruses can infect chickens and quails. Chickens and quails can shed the viruses and develop antibody response during the experiment period. The viruses can also transmit to contacted chickens and quails. In summary, our results confirmed that free-grazing ducks are important reservoir hosts of influenza A virus and the H1N3 and H11N9 viruses from free grazing ducks were able to infect and transmit to chickens and quails. Thus, the results from multi-years influenza A surveillance in free-grazing ducks, the genetic characteristics and the pathogenicity of the viruses in animal models provided useful information for influenza prevention and control measures both in animals and humans in the future.
DOI: 10.14457/cu.the.2019.114
title: Correlation of allergen specific immunoglobulin to house dust mites and commensal microbes in atopic dogs and identification of major allergen in dermatophagoides farinae

description: To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) or allergen-specific IgE serology testing (ASIS). ASIS is more practical, convenient and safer. However, the results of ASIS could be affected by source and type of allergen. Crude allergen extracts were still used in commercial testing and protein antigens of skin microbes were not included in the panel of testing. In this study, our group developed serology test for confirming house dust mite allergy called Dermatophagoides farinae and D. pteronyssinus, and measuring specific immunoglobulin to skin microbes on dogs like Malssezia pachydermatis yeast and Staphylococcus pseudintermedius bacteria. The results was found that high IgE levels to house dust mites could be used to differentiate atopic dog with allergy to house dust mites and atopic dogs without allergy to house dust mites. Moreover, IgG1 levels could also use to differentiate house dust mite allergy and non-house dust mite allergy by using lower sample volumes than IgE about 100 times. In the other hand, total IgG and IgG2 levels could not be used to differentiate house dust mite allergy and non-house dust mite allergy in atopic dogs. Both of IgE and IgG1 levels showed the same degrees of agreement to IDT. By investigation through two-dimensional IgE blotting, ten proteins of house dust mites that related to house dust mite allergy in atopic dogs were found, Der f 28 protein is interesting and should be selected to apply for a component-resolved diagnostics. Alpha-enolase, serine protease, and arginine kinase are the critical protein to be a cause of cross reaction. The results of specific immune response to skin microbes were found that atopic dogs had higher specific IgE levels to both skin microbes than healthy dogs. This indicated that skin microbes could act as allergen in atopic dogs. Other specific IgG1 and IgG2 to these skin microbes also high. However, none of any types specific immunoglobulin to skin microbes related to skin severity scores in atopic dogs determining through the Canine Atopic Dermatitis Lesion Index. We concluded that levels of specific immunoglobulin to skin microbes did not relate to skin severity.
DOI: 10.14457/cu.the.2019.115
title: Single fixed-time artificial insemination with fresh or frozen thawed semen in pig

description: Single fixed-time AI is the breeding protocol inseminating the sows once with either fresh or frozen-thawed (FT) semen at fixed time after ovulation induction without estrus detection. To develop the success single fixed-time AI protocol in Thailand, factors affecting the efficacy of single fixed-time AI were evaluated. Additionally, we also evaluated the effect of using lecithin in cryopreservation extender on FT semen qualities in order to find the possibility to perform single fixed-time AI using sperm preserved in lecithin based extender. It was found that administration of 10 ug buserelin at 72 h after weaning decreased weaning-to-ovulation interval compared to spontaneous ovulation (127.6 vs. 157.2 h). However, the response of buserelin injection was also affected by season and weaned sows conditions. Hot season prolonged ovulation time. Weaned sows with lactation length (LL) ≥20 days, litter weight (LW) &lt;67 kg, or body condition score (BCS) ≥3, or high backfat reserve after weaning had better buserelin responses. The single fixed-time AI using 10 ug buserelin injection at 72 h after weaning and insemination the sows at 32 h later was successfully performed under tropical climate with acceptable litter size compared to conventional AI (12.0 vs. 12.8 piglets per litter). A decreased litter size was also observed when single fixed-time AI was performed in the hot season or using intrauterine insemination (IUI) with a reduced number of sperm. Finally, the FT semen qualities of sperm preserved in cryopreservation extenders containing 20% egg yolk or 3% lecithin and 10% egg yolk were higher than those in 6% lecithin extender. In conclusion, the present study found that hot season decreased response of buserelin injection, delayed ovulation time and decreased sows reproductive performances after single fixed-time AI. Weaned sows with LL ≥20 days, LW &lt;67 kg, BCS ≥3 or high backfat had better responses to buserelin injection. Single fixed-time AI using the IUI with a reduced number of sperm decreased litter size. Replacing of egg yolk with lecithin in cryopreservation extender did not increase FT semen qualities. The precise concentration of lecithin should be further investigated to perform single fixed-time AI using FT semen preserved in lecithin-based extender.
DOI: 10.14457/cu.the.2018.472
title: The effect of monosodium glutamate on brain, liver, kidney, testis, ovary, fertilization and hatching rate in zebrafish (danio rerio)

description: Monosodium glutamate (MSG) is composed of sodium salt and amino acids that is commonly used to enhance food taste. Although it is safe at consumption level, the adverse effect of MSG had been reported. The present research was intended to evaluate the effect of MSG on the brain, liver, kidney, testes, and ovaries of zebrafish (Danio rerio) which were exposed from embryo stage to adult stage, along with the analyzing of the fertilization rate and hatching rate of zebrafish embryos. From the observation of histopathological changing of different organs of zebrafish after exposed to 10, 100, and 1000 ppm MSG for 60 days compared with control group (zebrafish in each group were mating before sacrificed for study the histopathology. In the next day embryos of zebrafish were collected and analyzed for the numbers, survival rate and hatching rate). The results indicated that liver and kidney in MSG exposed group had significantly higher lesion score (p-value &lt;0.05) compared to the control group. Generalized swelling of hepatocytes and blood congestion in the liver as well as the congestion and decreasing number of distal tubule in the kidney from all of the MSG exposed groups had been found. The severity of the lesions from 1000 ppm MSG exposure group was the highest following by the group of 100 and 10 ppm. However, no detectable lesions in the brain tissue, testes, and ovaries of every group were found. Zebrafish from the 1000 ppm MSG exposed group did not spawn after placing in the mating tank, while in 100 ppm MSG group was lower in the number of embryos than 10 ppm MSG and control group. The survival of fertilized embryos was observed every 24 hours for 120 hours. The result of survival rate in 100 ppm MSG group was significantly lower than the control group. All of zebrafish embryos that can be developed until 96 hours post fertilization were hatching completely. In summary, this study revealed that zebrafish exposed to MSG for 60 days showed liver and kidney lesions and reduced the reproductive performance.
DOI: 10.14457/cu.the.2019.116
title: Development of an indirect elisa to detect antibodies against actinobacillus suis using boiled whole cell antigen

description: Actinobacillus suis is considered an opportunistic pathogen in swine that can cause disease in animals of all ages, especially in high health status herds. The objective of this study was to developed an indirect enzyme-linked immunosorbent assay (ELISA) using boiled whole cell antigen for the detection of antibody against A. suis. The selected herd has clinical problems associated with A. suis infection. In addition, this herd was not received any Actinobacillus pleuropneumoniae (APP) vaccine nor diagnosed with APP infection before the study. The isolation of A. suis was recovered from lung of the infected pig in the selected herd. Moreover, the antigen of A. suis was prepared in form of boiled whole cell antigen for coated ELISA plate. Blood samples were collected from pigs in the same herd at 3, 5, 7, 9, 11, 12, 13, 14, 15, 17, 18, 20, 21-25 and 25 weeks of age. Moreover, blood collection was collected from Parity 0 (34-36 weeks of age), P1-P2, P3-P4, and P5 of sows for detection of antibodies against A. suis in indirect ELISA test. The ELISA was optimized by using checkerboard titration. As a result, the cut-off value was set at 0.36 (OD450). The higher level of optical density (OD) was observed when the pigs more than 20 weeks of age (p&lt;0.05). The highest level of OD related A. suis antibody was observed in the sows at the Parity 1-4 in which the level of the A. suis antibody was different to gilt aged less than 25 weeks (p&lt;0.01). In conclusion, the development of indirect ELISA technique may not be a gold standard or completely used as diagnostic tool due to the difficulty to detect the conserved antigenicity of A. suis antigen. It could be used as an alternative tool for study the dynamic serological profile of the suspected pigs infected with A. suis in the herd and these data could be applied to develop the appropriate vaccination protocol against these pathogens for herd health management.
DOI: 10.14457/cu.the.2018.474
title: Development of reverse transcription loop-mediated isothermal amplification combine with lateral flow device as a detection assay for swine influenza virus

description: Swine influenza causes high morbidity and low mortality in pigs. The disease spreads rapidly in pig farms causing the delay of pig weight to reach market. Moreover, pigs play important role as “mixing vessel” of influenza viruses. Pigs can support infection and replication of influenza viruses from avian, human and swine origins. These lead to major concerns of the important of swine influenza which are not only the economic impacts but also the human health. There is the need of accurate and rapid diagnosis for swine influenza virus (SIV) to minimize further spreading of the viruses in pig population and to reduce the risk of multiple infections of influenza viruses. The objective of this study was to develop a Reverse Transcription Loop-Mediated Isothermal Amplification combine with Lateral Flow Device (RT-LAMP-LFD) assay for the detection of SIVs and to evaluate the performance, sensitivity and specificity of the assay. This study was conducted in two phases: the development of RT-LAMP-LFD assay for SIVs detection and the evaluation of the performance, sensitivity and specificity of the assay. The result showed that RT-LAMP-LFD assay was developed with six newly designed primers and with optimum condition at 63oC and 30 minutes. The detection limit of the assay was 1.14 pg/µl, which is 10-fold higher than real time RT-PCR assay, RT-LAMP-LFD assay is specific method for the detection of SIV, there were no cross reaction with other important viruses of pigs (PRRSV, PCV2, Pseudorabies virus and PEDV). From our result the newly developed RT-LAMP-LFD assay has good performance with 100% sensitivity, 100% specificity and perfect percentage of agreement (Kappa = 1) compared to reference assays (viral isolation and/or real time RT-PCR). In conclusion, this RT-LAMP–LFD assay has a potential for patent technology as a rapid diagnostic test for SIV detection and could be developed for low-cost or in house kits. The RT-LAMP-LFD assay could be implemented and applied at first point care of SIV outbreaks to help control and prevent the spread of disease in pig farms.
DOI: 10.5281/zenodo.4876678
title: 2,700 simulated small INDELs and ITDs for MINTIE paper

description: Contains nine simulations, each containing 300 small INDELs and ITDs of a single size ranging from 1-9. Code used to generate these samples can be found under https://github.com/Oshlack/MINTIE/tree/master/simu. See the 1,500 variant data set and the MINTIE pre-print for simulation methodology.
DOI: 10.5281/zenodo.4876677
title: 2,700 simulated small INDELs and ITDs for MINTIE paper

description: Contains nine simulations, each containing 300 small INDELs and ITDs of a single size ranging from 1-9. Code used to generate these samples can be found under https://github.com/Oshlack/MINTIE/tree/master/simu. See the 1,500 variant data set and the MINTIE pre-print for simulation methodology.
DOI: 10.14457/cu.the.1998.170
title: Alcohol-free microemulsion formation with perchloroethylene and a gemini surfactant
DOI: 10.14457/cu.the.1998.171
title: Female community health volunteer training : a strategy to improve the case management of a cute respiratory infection in under 5 children in Nepal
DOI: 10.14457/cu.the.1998.172
title: Study on mechanical properties of starch-based HDPE blends
DOI: 10.14457/cu.the.1998.173
title: Flow and reaction of weak acids in carbonate porous media
DOI: 10.14457/cu.the.1998.174
title: Bonded fluorocarbon admicelles : production and adsolubilization properties
DOI: 10.14457/cu.the.1998.175
title: Processability and mechanical properties of HDPE film containing starch as a filler
DOI: 10.14457/cu.the.1998.176
title: Study on the benzoxazine monomers and their application for ion extraction material
DOI: 10.14457/cu.the.1998.177
title: Task-based learning with early community exposure : an education program to improve community orientation of medical students in Myanmar
DOI: 10.6084/m9.figshare.16643015
title: Supplementary figures from The control effort to steer self-propelled microswimmers depends on their morphology: comparing symmetric spherical versus asymmetric <i>L</i>

description: Supplementary figures providing a deeper understanding of the methods used.
DOI: 10.6084/m9.figshare.16643015.v1
title: Supplementary figures from The control effort to steer self-propelled microswimmers depends on their morphology: comparing symmetric spherical versus asymmetric <i>L</i>

description: Supplementary figures providing a deeper understanding of the methods used.
DOI: 10.6084/m9.figshare.16643005
title: Supplementary 2 Relation extraction result

description: <b>Bipolar Disorder Content Analysis</b>
DOI: 10.21227/fdwv-4d64
title: Pentesting Methodology

description: The security testing focuses on evaluating the security of the web, mobile, networks, API, SaaS, blockchain & cloud applications by methodically validating & verifying the effectiveness of security controls. The process involves an active analysis of any application for any available weaknesses, technical flaws, or vulnerabilities.The security audit scope of work will include:Vulnerability Assessment and Penetration Testing (VAPT)Static dynamic code analysisTechnical assistance in patching found security vulnerabilitiesCollaborative cloud dashboard for vulnerability reporting managementAccess to our security tools/APIsConsultation on the best security practices for your applicationGenerally, the black box testing include automated pentesting via tools and by an external team (3rd party)
DOI: 10.6084/m9.figshare.15154714
title: De Baets et al Supplementary Table 2 from Phanerozoic parasitism and marine metazoan diversity: dilution versus amplification

description: Diversity data used to perform analyses
DOI: 10.6084/m9.figshare.15154717
title: De Baets et al Supplementary Table 1 from Phanerozoic parasitism and marine metazoan diversity: dilution versus amplification

description: Raw data used to perform analyses
DOI: 10.6084/m9.figshare.15154717.v2
title: De Baets et al Supplementary Table 1 from Phanerozoic parasitism and marine metazoan diversity: dilution versus amplification

description: Raw data used to perform analyses
DOI: 10.6084/m9.figshare.15154717.v1
title: De Baets et al Supplementary Table 1 from Phanerozoic parasitism and marine metazoan diversity: dilution versus amplification

description: Raw data used to perform analyses
DOI: 10.6084/m9.figshare.16571019
title: draft

description: try to use figshare
DOI: 10.6084/m9.figshare.16571019.v3
title: draft

description: try to use figshare
DOI: 10.6084/m9.figshare.16571019.v2
title: Workplace ergonomics problems and solutions: Working from home

description: A qualitative ethnographic methodology was adopted. This study included a focus group discussion among experts from the fields of higher education, healthcare, human resources (HR), and ergonomics patient in Malaysia. The most common ergonomic issues identified were based on diagnoses and observations in previous studies
DOI: 10.6084/m9.figshare.12594914
title: Wild Harbor Porpoise Heart Rate and Behavior

description: The impressive breath-hold capabilities of marine mammals are facilitated by both enhanced O2 stores and reductions in the rate of O2 consumption via peripheral vasoconstriction and bradycardia, coined the dive response. Many studies have focused on the extreme role of the dive response in maximizing dive duration in marine mammals, but few have addressed how these adjustments may compromise the capability to hunt, digest and thermoregulate during routine dives. Here we use DTAGs which record heart rate together with foraging and movement behaviour to investigate how O2 management is balanced between the need to dive and forage in five wild harbour porpoises that hunt thousands of small prey daily during continuous shallow diving. Dive heart rate was moderate (median minimum 47-69 bpm) and relatively stable across dive types, dive duration (0.5-3.3 min), and activity. A moderate dive response, allowing for some perfusion of peripheral tissues, may be essential for fuelling the high field metabolic rates required to maintain body temperature and support digestion during diving in these small, continuously-feeding cetaceans. Thus, despite having the capacity to prolong dives via a strong dive response, for these shallow-diving cetaceans, it appears to be more efficient to maintain circulation while diving: extreme heart-rate gymnastics are for deep dives and emergencies, not everyday use.<br><br>This dataset is associated with a manuscript submitted to Proceedings of the Royal Society B investigating diving heart rate of wild harbor porpoises.<br>There are 13 files. 1) McDonaldetal_Porpoise2021_ColumnHeader_Def.csv - provides column name definitions for all the file types. 2) DiveSummaryStats_Porpoise_2021.csv - Heart rate, activity and dive summary data for all dives analyzed in the paper. All five porpoises combined3) IntervalSummaryStats_Porpoise_2021.csv - Summary heart rate and activity data for 5 s intervals during the bottom of the dive. All 5 porpoises combined.4-8) Files that end with HR. The instantaneous heart rate data for the 5 porpoises in the study. File name includes porpoise ID. 9-14) Files that end with MSA. A MSA data file for each of the 5 porpoises with minimum specific acceleration data at 25 Hz. File name includes porpoise ID.Large raw and processed tag data are available upon request to Birgitte McDonald (gmcdonald@mlml.calstate.edu)<br>
DOI: 10.6084/m9.figshare.12594914.v1
title: Wild Harbor Porpoise Heart Rate and Behavior

description: The impressive breath-hold capabilities of marine mammals are facilitated by both enhanced O2 stores and reductions in the rate of O2 consumption via peripheral vasoconstriction and bradycardia, coined the dive response. Many studies have focused on the extreme role of the dive response in maximizing dive duration in marine mammals, but few have addressed how these adjustments may compromise the capability to hunt, digest and thermoregulate during routine dives. Here we use DTAGs which record heart rate together with foraging and movement behaviour to investigate how O2 management is balanced between the need to dive and forage in five wild harbour porpoises that hunt thousands of small prey daily during continuous shallow diving. Dive heart rate was moderate (median minimum 47-69 bpm) and relatively stable across dive types, dive duration (0.5-3.3 min), and activity. A moderate dive response, allowing for some perfusion of peripheral tissues, may be essential for fuelling the high field metabolic rates required to maintain body temperature and support digestion during diving in these small, continuously-feeding cetaceans. Thus, despite having the capacity to prolong dives via a strong dive response, for these shallow-diving cetaceans, it appears to be more efficient to maintain circulation while diving: extreme heart-rate gymnastics are for deep dives and emergencies, not everyday use.<br><br>This dataset is associated with a manuscript submitted to Proceedings of the Royal Society B investigating diving heart rate of wild harbor porpoises.<br>There are 13 files. 1) McDonaldetal_Porpoise2021_ColumnHeader_Def.csv - provides column name definitions for all the file types. 2) DiveSummaryStats_Porpoise_2021.csv - Heart rate, activity and dive summary data for all dives analyzed in the paper. All five porpoises combined3) IntervalSummaryStats_Porpoise_2021.csv - Summary heart rate and activity data for 5 s intervals during the bottom of the dive. All 5 porpoises combined.4-8) Files that end with HR. The instantaneous heart rate data for the 5 porpoises in the study. File name includes porpoise ID. 9-14) Files that end with MSA. A MSA data file for each of the 5 porpoises with minimum specific acceleration data at 25 Hz. File name includes porpoise ID.Large raw and processed tag data are available upon request to Birgitte McDonald (gmcdonald@mlml.calstate.edu)<br>