DOI: 10.5061/dryad.v9s4mw6qd
title: Data from: Genomics detects population structure within and between ocean basins in a circumpolar seabird: the white-chinned petrel

description: The Southern Ocean represents a continuous stretch of circumpolar marine
 habitat, but the potential physical and ecological drivers of evolutionary
 genetic differentiation across this vast ecosystem remain unclear. We
 tested for genetic structure across the full circumpolar range of the
 white-chinned petrel (Procellaria aequinoctialis) to unravel the potential
 drivers of population differentiation and test alternative population
 differentiation hypotheses. Following range-wide comprehensive sampling,
 we applied genomic (genotyping-by-sequencing or GBS; 60,709 loci) and
 standard mitochondrial-marker approaches (cytochrome b and 1st domain of
 control region) to quantify genetic diversity within and among island
 populations, test for isolation by distance, and quantify the number of
 genetic clusters using neutral and outlier (non-neutral) loci. Our results
 supported the multi-region hypothesis, with a range of analyses showing
 clear three-region genetic population structure, split by ocean basin,
 within two evolutionary units. The most significant differentiation
 between these regions confirmed previous work distinguishing New Zealand
 and nominate subspecies. Although there was little evidence of structure
 within the island groups of the Indian or Atlantic oceans, a small set of
 highly-discriminatory outlier loci could assign petrels to ocean basin and
 potentially to island group, though the latter needs further verification.
 Genomic data hold the key to revealing substantial regional genetic
 structure within wide-ranging circumpolar species previously assumed to be
 panmictic.
description: Rexer-Huber_WCP_cytb.fasta
 is haplotypes of the mitochondrial cytochrome b haplotypes (~1200 bp) for
 white-chinned petrels, produced via Sanger sequencing on an ABI 3730xl DNA
 analyser. Rexer-Huber_cytb_haplotype-sample_table.xlsx links haplotype names to the original petrel samples. Rexer-Huber_WCP_CR1D.fasta is haplotypes of the first domain of the mitochondrial control region (~559 bp) for white-chinned petrels, produced by Sanger sequencing on an ABI 3730xl DNA analyser. Haplotypes are named according to sample and location of origin. File Rexer-Huber_GBS_s_4_fastq.txt.gz is the raw reads produced by genotyping-by-sequencing. Genomic DNA was digested with PstI and MspI. Size selection to 193-500 bp. Library sequenced on Illumina HiSeq2500 using single-end reads, with 101 cycles in high-output mode (v4 chemistry). Rexer-Huber_GBS_barcodes.txt is the barcodes. Further datasets are stored on the Molecular Ecology website as Supporting Data files with the publication. These are: Supp Data A WCP outliers.xlsx (outputs from outlier analyses), Supp Data B WCP fulmar map.xlsx (loci mapped to the northern fulmar genome), and Supp Data C WCP outliers combined genepop.txt (outlier loci for GenePop analyses).
description: A ReadMe is provided to identify datasets, define abbreviations,
 and describe related datasets stored elsewhere.
 Rexer-Huber_cytb_haplotype-sample_table.xlsx links cytochrome b
 haplotype names to the original petrel samples.
 Throughout, abbreviations for sampling location are: ANT
 Antipodes; CAM Campbell; AKL Auckland; SG South Georgia; FK Falkland
 Islands; CRO Crozet; MAR Marion; KER Kerguelen; AIO haplotype ocurring
 across more than one Atlantic-Indian Ocean island.