DOI: 10.5061/dryad.d6t40
title: Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja

description: Extant deep-sea invertebrate fauna represent both ancient and recent
 invasions from shallow-water habitats. Hydrostatic pressure may present a
 significant physiological challenge to organisms seeking to colonize
 deeper waters or migrate ontogenetically. Pressure may be a key factor
 contributing to bottlenecks in the radiation of taxa and potentially drive
 speciation. Here, we assess shifts in the tolerance of hydrostatic
 pressure through early ontogeny of the northern stone crab Lithodes maja,
 which occupies a depth range of 4–790 m in the North Atlantic. The zoea I,
 megalopa and crab I stages were exposed to hydrostatic pressures up to
 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of
 genes putatively coding for the N-methyl-d-aspartate receptor-regulated
 protein 1 (narg gene), two heat-shock protein 70 kDa (HSP70) isoforms and
 mitochondrial Citrate Synthase (CS gene) were measured. This study finds a
 significant increase in the relative expression of the CS and hsp70a genes
 with increased hydrostatic pressure in the zoea I stage, and an increase
 in the relative expression of all genes with increased hydrostatic
 pressure in the megalopa and crab I stages. Transcriptional responses are
 corroborated by patterns in respiratory rates in response to hydrostatic
 pressure in all stages. These results suggest a decrease in the acute
 high-pressure tolerance limit as ontogeny advances, as reflected by a
 shift in the hydrostatic pressure at which significant differences are
 observed.
description: Narg FASTA files for
 alignmentFASTA format files used
 to derive the narg gene alignmentNarg_FASTA_files_for_alignment.txtClustalO narg alignmentClustal Omega alignment file for the narg geneclustalo_narg_alignment.clustalRpl8 FASTA files for alignmentFASTA files used to derive the rpl8 alignmentRpl8_FASTA_files_for_alignment.txtClustalO rpl8 alignmentClustal Omega download file for rpl8 alignmentclustalo_rpl8_alignment.clustalTubulin FASTA files for alignmentFASTA files used to derives Tubulin gene alignmentTubulin_FASTA_files_for_alignment.txtClustalO tubulin alignmentClustal Omega download for tubulin gene alignmentclustalo_tubulin_alignment.clustalEef1a FASTA file alignmentsFASTA files used to derive eef1a gene alignmentEef1a_FASTA_file_alignments.txtClustalO eef1a alignmentClustal Omega download for eef1a gene alignmentclustalo_eef1a_alignment.clustalCS FASTA files for alignmentFASTA files used to derive CS gene alignmentCs_FASTA_files_for_alignment.txtClustalO cs alignmentClustal Omega download file for CS gene alignmentclustalo_cs_alignment.clustalHsp70a FASTA files for alignmentFASTA files used to derive HSP70a gene alignmentHsp70a_FASTA_files_for_alignment.txtClustalO hsp70a alignmentClustal Omega download file for hsp70a gene alignmentclustalo_hsp70a_alignment.clustalHsp70b FASTA files for alignmentFASTA files used to derive hsp70b gene alignmentHsp70b_FASTA_files_for_alignment.txtClustalO hsp70b alignmentClustal Omega download for hsp70b gene alignmentclustalo_hsp70b_alignment.clustal