DOI: 10.5061/dryad.qnk98sfv9
title: Data from: Does immune priming in Galleria mellonella reveal plastic mechanisms for survival?

description: This dataset includes physiological and survival data from an immune
 priming experiment conducted on two populations of lepidopteran larvae
 originating from Siberia (Palearctic) and Mexico (Neotropical). The moths
 were bred for five generations under controlled laboratory conditions
 (30 ± 1°C, 30% RH, darkness) before immune assays were performed. Larvae
 were size-controlled (17–20 mm) and maintained individually to avoid batch
 effects. Dataset includes: 1) Phenoloxidase (PO) activity: Measured via
 dopachrome formation at 490 nm from L-DOPA substrate over one hour in two
 populations: Mexico and Siberia, with two experimental groups: Sham and
 primed 2) Lytic activity: Measured via turbidity reduction at 540 nm from
 M. lysodeikticus suspension over 30 minutes in two populations: Mexico and
 Siberia, with two experimental groups: Sham and primed 3) Hemocyte count:
 Total and live hemocyte numbers measured via trypan blue exclusion using
 the TC20™ Automated Cell Counter in two populations: Mexico and Siberia,
 with two experimental groups: Sham and primed 4) Mortality assay:
 Mortality outcomes following immune priming and bacterial challenge in
 larvae from two biogeographic origins, Mexico and Siberia, having two
 experimental groups: Sham and primed
description: # Does immune priming in Galleria mellonella reveal plastic mechanisms for
 survival? Dataset DOI: [10.5061/dryad.qnk98sfv9](10.5061/dryad.qnk98sfv9)
 ## Description of the data and file structure Hemocyte
 data: Quantification of circulating hemocytes per individual lava in two
 populations- Mexico and Siberia, after Sham vs Priming treatments PO
 activity data: Measurement of phenoloxidase activity over time using
 absorbance at 490 nm in hemolymph samples from Mexico and Siberia
 populations, under Sham and Priming treatments. Survival data: Binary
 survival outcomes (alive or dead) recorded 24 hours post-pathogen
 challenge in Sham vs. Primed individuals from both the Mexico and Siberia
 populations. 1 stands for alive, and 0 stands for dead. Lytic activity
 data: Quantification of antimicrobial lytic activity by measuring the
 decline in absorbance at 540 nm over time after adding Micrococcus
 lysodeikticus to hemolymph samples, in Sham and Primed individuals from
 both populations. ### Files and variables #### File: Hemocytes\_data.xlsx
 **Description:** Quantification of circulating total and live hemocytes
 per individual larva in two populations. ##### Variables * Populations-
 Mexico and Siberia * Treatments (groups)- Control and Primed, (n/a- not
 applicable, caterpillar removed from the treatment group due to handling
 error)  #### File: Lytic\_activity.xlsx **Description:** Measurement of
 phenoloxidase activity over time using absorbance at 490 nm #####
 Variables * Populations- Mexico and Siberia * Treatments (groups)- Control
 and Primed #### File: Survival.xlsx **Description:** Binary survival
 outcomes (alive or dead) recorded 24 hours post-pathogen challenge #####
 Variables * Populations- Mexico and Siberia * Treatments (groups)- Sham
 and Primed #### File: PO\_activity.xlsx **Description:** Measurement of
 phenoloxidase activity over time using absorbance at 490 nm #####
 Variables * Populations- Mexico and Siberia * Treatments (groups)- Control
 and Primed