cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.3531952
title: DATA SETS: Are tropical reptiles really declining? A six-year survey of snakes in Drake Bay, Costa Rica, and the role of temperature, rain and light

description: Raw data sets of the article: Are tropical reptiles really declining? A six-year survey of snakes in Drake Bay, Costa Rica, and the role of temperature, rain and light ABSTRACT <strong>Introduction:</strong> Even though snake declines seem to be a reality in many parts of the world, some reports are based on anecdotal evidence and there is a need of prolonged and intensive studies, especially in the tropics, for corroboration. <strong>Objective:</strong> To<strong> </strong>investigate if snake populations in Drake Bay are decreasing, and if there is a relationship with prey, time, temperature, rain and moonlight. <strong>Methods:</strong> We counted snakes seen per hour when walking along a single trail in the coastal forest of Drake Bay, Costa Rica. We walked the trail at night for a total of 842 nights (over 4 000 hours of observations), from 2012 through 2017 and recorded all the individual snakes we could see with head flashlights. We used ANOVA tests to check correlations among counts per hour with moonlight and rain; and graphic analysis for associations with diet, temperature, month and year. <strong>Results:</strong> We recorded 25 species (five families); they feed mostly on terrestrial vertebrates. Counts per hour have fallen over the years, especially for species that prey on amphibians and reptiles; <em>Mastigodryas melanolomus</em> has remained in similar numbers; and <em>Siphlophis compressus</em> has not been seen since May 2016. Temperature is relatively constant along the year in Drake, but month strongly affected the counts, which increased from August to September. Most species were seen more often in <em>rainy nights</em> (0,11 per hour versus only 0,03 per hour in nights without rain, ANOVA p&lt;0.05); we saw less <em>Leptodeira septentrionalis</em> on bright nights (0.12 per hour, versus 0,21 per hour in dark nights; ANOVA, p=0.01), but all other species were unaffected by moonlight (ANOVA, P &gt;0.05). <strong>Conclusion</strong>: Night field counts of snakes in Drake Bay, Costa Rica, are not strongly affected by light or temperature, but are lower when there is no rain and show a strong decline from 2012 through 2017, particularly for species that feed on amphibians and reptiles. We have no reason to believe that the decline is an erroneous interpretation or that the snakes moved elsewhere, the decline of snakes in Drake seems to be real and needs attention from the conservation authorities.
DOI: 10.5281/zenodo.3531953
title: DATA SETS: Are tropical reptiles really declining? A six-year survey of snakes in Drake Bay, Costa Rica, and the role of temperature, rain and light

description: Raw data sets of the article: Are tropical reptiles really declining? A six-year survey of snakes in Drake Bay, Costa Rica, and the role of temperature, rain and light ABSTRACT <strong>Introduction:</strong> Even though snake declines seem to be a reality in many parts of the world, some reports are based on anecdotal evidence and there is a need of prolonged and intensive studies, especially in the tropics, for corroboration. <strong>Objective:</strong> To<strong> </strong>investigate if snake populations in Drake Bay are decreasing, and if there is a relationship with prey, time, temperature, rain and moonlight. <strong>Methods:</strong> We counted snakes seen per hour when walking along a single trail in the coastal forest of Drake Bay, Costa Rica. We walked the trail at night for a total of 842 nights (over 4 000 hours of observations), from 2012 through 2017 and recorded all the individual snakes we could see with head flashlights. We used ANOVA tests to check correlations among counts per hour with moonlight and rain; and graphic analysis for associations with diet, temperature, month and year. <strong>Results:</strong> We recorded 25 species (five families); they feed mostly on terrestrial vertebrates. Counts per hour have fallen over the years, especially for species that prey on amphibians and reptiles; <em>Mastigodryas melanolomus</em> has remained in similar numbers; and <em>Siphlophis compressus</em> has not been seen since May 2016. Temperature is relatively constant along the year in Drake, but month strongly affected the counts, which increased from August to September. Most species were seen more often in <em>rainy nights</em> (0,11 per hour versus only 0,03 per hour in nights without rain, ANOVA p&lt;0.05); we saw less <em>Leptodeira septentrionalis</em> on bright nights (0.12 per hour, versus 0,21 per hour in dark nights; ANOVA, p=0.01), but all other species were unaffected by moonlight (ANOVA, P &gt;0.05). <strong>Conclusion</strong>: Night field counts of snakes in Drake Bay, Costa Rica, are not strongly affected by light or temperature, but are lower when there is no rain and show a strong decline from 2012 through 2017, particularly for species that feed on amphibians and reptiles. We have no reason to believe that the decline is an erroneous interpretation or that the snakes moved elsewhere, the decline of snakes in Drake seems to be real and needs attention from the conservation authorities.
DOI: 10.21227/004e-v304
title: Malware Analysis Datasets: Top-1000 PE Imports

description: This dataset is part of my PhD research on malware detection and classification using Deep Learning. It contains static analysis data: Top-1000 imported functions extracted from the 'pe_imports' elements of Cuckoo Sandbox reports. PE malware examples were downloaded from virusshare.com. PE goodware examples were downloaded from portableapps.com and from Windows 7 x86 directories.
DOI: 10.21227/799r-m573
title: Malware Analysis Datasets: Top-1000 PE Imports

description: This dataset is part of my PhD research on malware detection and classification using Deep Learning. It contains static analysis data: Top-1000 imported functions extracted from the 'pe_imports' elements of Cuckoo Sandbox reports. PE malware examples were downloaded from virusshare.com. PE goodware examples were downloaded from portableapps.com and from Windows 7 x86 directories.
DOI: 10.5281/zenodo.3532001
title: Replication Data for: (111)-oriented, single crystal diamond tips for nanoscale scanning probe imaging of out-of-plane magnetic fields

description: Data repository for: <strong>(111)-oriented, single crystal diamond tips for nanoscale scanning probe imaging of out-of-plane magnetic fields</strong> <em>Data description.pdf </em>describes the uploaded data.<br> <em>Data.xlsx</em> is the data represented in the paper.
description: We gratefully acknowledge financial support through the NCCR QSIT (Grant No. 185902), the Basel QCQT doctoral school, through the EU Quantum Flagship project ASTERIQS (Grant No. 820394), and through the Swiss NSF Project Grants Nos. 169321 and 155845.
DOI: 10.5281/zenodo.3532002
title: Replication Data for: (111)-oriented, single crystal diamond tips for nanoscale scanning probe imaging of out-of-plane magnetic fields

description: Data repository for: <strong>(111)-oriented, single crystal diamond tips for nanoscale scanning probe imaging of out-of-plane magnetic fields</strong> <em>Data description.pdf </em>describes the uploaded data.<br> <em>Data.xlsx</em> is the data represented in the paper.
description: We gratefully acknowledge financial support through the NCCR QSIT (Grant No. 185902), the Basel QCQT doctoral school, through the EU Quantum Flagship project ASTERIQS (Grant No. 820394), and through the Swiss NSF Project Grants Nos. 169321 and 155845.
DOI: 10.21227/88k9-1z60
title: Malware Analysis Datasets: Raw PE as Image

description: This dataset is part of my PhD research on malware detection and classification using Deep Learning. It contains static analysis data: Raw PE byte stream rescaled to a 32 x 32 greyscale image using the Nearest Neighbor Interpolation algorithm and then flattened to a 1024 bytes vector. PE malware examples were downloaded from virusshare.com. PE goodware examples were downloaded from portableapps.com and from Windows 7 x86 directories.
DOI: 10.26300/62ps-mv85
title: feronia_Athaliana_x_myb_Athaliana_Rep_07_082719
DOI: 10.17632/j59z2397st.1
title: Data for: Removal of Cd(II) from aqueous solutions using plant-derived biochar: Kinetics, Isotherm and Characterization
DOI: 10.26300/g3fw-9c73
title: feronia_Athaliana_x_myb_Athaliana_Rep_09_082719
DOI: 10.26300/y5gk-a118
title: feronia_Athaliana_x_myb_Athaliana_Rep_08_082719
DOI: 10.14455/tsb.res.2019.3
title: Anti-Breast Cancer Activity of Thai Herbal Medicine Formulation

description: Thai traditional medicine formulations have been used as folk medicine to treat breast cancer patients, balance element in the patient's body or increase their immunities. It is composed of five Thai medicinal plants
description: Albizia procera bark, Amphineurion marginatum root and stem, Diospyros mollis heartwood, Smilax corbularia and Smilax glabra rhizome. Nonetheless, biological testing for anti-cancer activity against breast cancer and biological effect of this formulation on cell line models have not yet been fully investigated. This research aimed to identify species of plant materials and investigate cytotoxic effects of the formulation and its constituent plants on normal mouse fibroblast (L929) and human breast cancer (MCF-7) cells. Phylogenetic identification of plant materials was evaluated using genetic markers on chloroplast DNA. Two non-coding regions
description: trnL intron and trnLF IGS, were amplified by PCR technique using universal primers. These PCR products were identified by DNA sequencing and aligned with ClustalW using BioEdit program. The nucleotide sequences were submitted to GenBank database and accession numbers were assigned. BLAST results of A. procera and A. marginatum showed the highest percent identities at 100% in both noncoding regions when compared with reference sequences on GenBank database. Then, ethanol maceration was performed which was similar to those practiced by Thai folk preparation. As the results, ethanolic extract of formulation had potent anti- cancer activity on MCF- 7 cells and low cytotoxicity on L929 cells with IC50 values of 52.51 and 81.48 ?g/mL, respectively. These results will support the biological evidences of Thai folk medicines to treat breast cancer cells.
DOI: 10.14455/tsb.res.2019.4
title: Antimicrobial Activities of Plant Extracts Against Bacteria Associated with Bovine Mastitis

description: Bovine mastitis is mainly caused by bacterial infection of cow's udders, resulting in milk of lower quality. Antibiotic treatment of mastitis can leave antibiotic residues in milk and can increase antibiotic resistant bacteria in the food chain. The aims of this study were to investigate antimicrobial activities of selected natural extracts from traditional Thai plants against bovine mastitis-associated bacteria, in order to evaluate the possibility of using plant extracts as alternatives to antibiotics for bovine mastitis treatment and control. Rhizome and leaf plants (two types for each category) were chosen in this study, including Curcuma mangga (Mango ginger), Zingiber montanum (J. Koenig) Link ex A. Dietr. (Cassumunar ginger), Azadirachta indica A. Juss (Neem leaf) and Piper betle Linn. (Betel Pepper leaf). Antimicrobial activities of the aqueous and 70% ethanolic extracts of these plants were determined against 12 species of bovine mastitis-associated genera: Staphylococcus and Streptococcus, using disc diffusion, MIC and MBC assays. Extracts from P. betle and C. mangga showed inhibitory effects against the mastitis-associated bacterial isolates/strains tested. The 70% ethanolic extract of P. betle exhibited the highest antimicrobial activities, having the inhibition zone of 8.00?0.00 to 24.33?0.58 mm. The MIC and MBC values of the P. betle extract ranged from 0.07 to 1.17 and 0.29 to 9.38 mg/ml, respectively. P. betle extracts also had wide scope of inhibition against all bacterial species tested, and particularly displayed promising inhibitory effects against Staphylococcus aureus, one of the most significant infectious and drug-resistant bacteria in dairy farming. These results will be used for further development of natural therapeutic products for bovine mastitis treatment and control, which is hoped to be alternative products to commercial antibiotics.
DOI: 10.14455/tsb.res.2019.5
title: Antioxidant activity of porcine placenta and its cytotoxic effect on HaCaT cell

description: Porcine placenta, previously a low-value waste from pig farm, is now being popular material in supplementary food and cosmetic products. Porcine placenta (PP) is claimed to be beneficial for skin improvement as whitening and anti-wrinkle. This research aims to investigate properties of PP and also its effect on the immortalized human keratinocyte (HaCaT) cells including protein content, protein profiles, antioxidant activity, cytotoxic effect, and ability to promote cell proliferation. The protein content of PP measured by Lowry assay was 27.04 %(w/w). The ABTS assay was used to determine the antioxidant activity of PP. The results suggested that at concentration of 5 mg/ml, PP provides 83.6% of antioxidant activity. The cytotoxic effect of PP was studied on HaCaT cells using MTT assay. The results suggested that PP at a concentration of up to 0.1 mg/ml does not affect the cell viability. In addition, at 0.01 and 0.1 mg/ml, PP tends to promote cell proliferation. These results can be beneficial for further study of bioactivity of porcine placenta in order to apply in pharmaceutical and cosmetic aspects
DOI: 10.14455/tsb.res.2019.6
title: Antioxidative Activities and Total Phenolic and Flavonoid Contents of Extracts from Cordyceps millitaris Cultivation on Streamed Jasmine Rice Mixed Rice Berry

description: The free radicals are an atom, molecule or ions with unpaired electrons. They will destroy cells resulting in cell damage which can cause the disease and may lead to human death, ie, heart disease, cirrhosis, cataracts, cerebral palsy, premature ageing, cell or cancer mutations, and other diseases (Temple, 2000). Currently, human produces and uses synthetic drugs as clinical treatments but it may be side effects in the human bodies, such as the kidney or liver work hard, headaches, shaky, sexual dysfunction, etc (Lobo, et al. 2010). Therefore, scientists have studied and researched the drug that can improve a good effect on the human body. Because the Cordyceps has active substances that are good and beneficial to the body, such as reduce blood sugar increases metabolic processes, nutrients or metabolism of the body, help in the area of the familiar system, sexual enhancement and help in other matters properties that have a beneficial effect on the body (Zuo, et al., 2015). Moreover, Cordyceps millitaris can enhance the immune system, hormone balance, anticancer, antiaging, etc. The Cordyceps is difficult to cultivate, therefore, scientist researched suitable material for Cordyceps millitaris cultivation. We found that Jasmine rice and Rice Berry that is suitable for culturing. Both types of rice are the mainstay of Thai people and in Asia. Especially,the Rice Berry is the most popular in Thailand becauseis purple colour. It is a registered rice variety from Thailand, a cross-breed of Jao Hom Nin (JHN), a local non-glutinous purple rice and Khoa Dawk Mali 105 (hom Mali rice). The nutritional properties are anthocyanin stability, and physical and cooking properties. The outcome is a deep purple whole grain rice with softness and a palatable after taste. Cordyceps is relatively quite expensive with high market demand. Currently, the Cordyceps is medicine as commercially pellets available. We confirmed that the cordyceps suit for the human body by tested in human body temperature and the solvents that nontoxic to human. Cordyceps millitaris can also be cultured with rice such as Jasmine rice and Rice berry. Normally, cordyceps medium is thrown away at the end of the process, after harvest.
DOI: 10.14455/tsb.res.2019.7
title: Biochemical property of an endo-glucanase-like enzyme from Clostridium sp. Z-7026

description: Clostridium sp. Z-7026 is a cellulose-degrading anaerobic bacterium, and its genome was recently sequenced. By Blast analysis against the NCBI protein database, one gene encoding a cellulase-like protein was identified. This deduced protein was named Cel_2759, and the Cel_2759 encoding gene was cloned, expressed in Escherichia coli, and purified. The purified recombinant protein had an estimated size of 107 kDa. To reveal its hydrolyzing ability, five substrates, namely carboxymethylcellulose (CMC), regenerate amorphous cellulose (RAC), crystalline cellulose Avicel beechwood xylan (BWX), and pretreated rice straw were used for activity assay. Results showed that, with in 15 min, Cel_2759 actively hydrolyzed CMC, yielding a reducing sugar concentration of 689.45 ?g/mL. However, activities against RAC, BWX and rice straw required pronged incubation time up to 16 h, yielding reducing sugar concentrations of 73.32 ?g/mL, 1393.05 ?g/mL, 875.704 ?g/mL, respectively. At 24 h, activity against Avicel was not observed, suggesting that Cel_2759 was unable to hydrolyze crystalline cellulose
description: by contrast, it preferred soluble long chain substrate CMC for hydrolysis. Using CMC as the substrate, Cel_2759 had its own optimal pH and temperature for its biological function at pH 7.0-8.0 and 55 ?C. The hydrolysis products from CMC by Cel_2759 were a mixture of cellooligomers, suggesting that Cel_2759 was an endo-acting enzyme.
DOI: 10.7910/dvn/o35fw8/83qkpk
title: data.csv

description: :unav
DOI: 10.14455/tsb.res.2019.10
title: Characterization of fusing CBM6 within Penicillium oxalicum endo-1,4-beta-xylanase GH11 and its effectiveness on insoluble degradation

description: Xylanase is rapidly gaining attention as suitable for applications in a wide variety of industrial processes such as food additives, animal feed, pulp bleaching and ethanol or xylitol production. Endo-1,4-beta-xylanase GH11 in Pichia pastoris expression, xyn11- CBM1 and xyn11- CBM1- CBM6 were constructed and characterized to investigate the influence of CBM6 on Penicillium oxalicum. Multiple copy genes of recombinant Pichia strains were selected on YPD agar + 2,000 ? g/ mL of ZeocinTM and xylan- Congo red clearance plate assay. Highest activities of Xyn11-CBM1 and Xyn11-CBM1CBM6 were 39.07? 0.79 and 47.48? 0.33 units/mg protein, respectively at optimal conditions (citrate buffer pH 5.0, at 45?C). Xyn11-CBM1 and Xyn11CBM1-CBM6 showed an acidic-stable property at pH 4.0-6.0 and 4.0-6.5, respectively ( more than 100% of activity remaining) when kept at room temperature (RT) for 240 minutes. Xyn11-CBM1-CBM6 exhibited more than 85% and 75% of activity remaining in phosphate buffer pH 6.0 at 45?C for 60 and 120 minutes, respectively and significantly higher than Xyn11-CBM1. The activity of both enzymes was strongly inhibited by 1 mM of Hg2+and showed over-estimated sizes (about 22.96 and 57.36 kDa) when separated by SDS- PAGE and zymogram analysis. Degradation of complex materials indicated that the highest reducing sugar release was produced by Xyn11 CBM1-CBM6 activity. TLC analysis revealed that xylobiose, xylotriose and xylotetraose were produced by both enzymes when they hydrolyzed corncob, corn leaves and stalks, and rice straw. Results indicated that Xyn11-CBM1CBM6 was a suitable candidate for application in various industries.
DOI: 10.14455/tsb.res.2019.11
title: Comparison of Non-aeration and Aeration Malting Process on Amylase Activities in Rice Malt

description: The comparison of non-aeration and aeration during steeping and germination of rice malt was examined in this research. The weight gain, alpha-amylase, beta-amylase activities and the total reducing sugar in rice malt was determined and the scanning electron microscope of starchy endosperm presented a modification of malted grain. The alpha-amylase activity in rice malt from aeration process was higher than that found in non-aeration processes significantly (p&lt;0.05) at the first 5 days
description: whereas, beta-amylase activity was found in the same range. However, the total reducing sugar extracted from aeration process malt was greater than that found in the nonaeration process malt, which could support shoot and root growing and caused more final weight gain of aeration process malt. The eroded starch granules and pin hole endosperm could be detected in the rice malts. Therefore, the aeration process could stimulate strong germination rate, amylolytic enzyme activities and starchy endosperm modification.
DOI: 10.14455/tsb.res.2019.12
title: Comparison of pilot- and laboratory-scale centrifuge recovery and dewatering performances on beta-glucan production

description: Beta-glucan production involves separation of yeast cell wall from soluble cellular components and centrifugation is commonly employed. Poor liquid-solid separation and solid recovery, however, are common obstacles during process scale- up. In this study, the recovery and dewatering performance of floor-type centrifuge (Beckman J-6M/E) and pilot scale discstack centrifuge (Westfalia separator SA1-01-175 with Double Centripetal Pump) were compared. Centrifuges at both scales were used to separate cell wall from yeast autolysate as well as to wash cell wall. Centrifugation of yeast autolysate and homogenate (500 mL) in a 1-L bucket at 3500 rpm for 40 min with 15% w/w, the floor-type centrifuge could achieve 30% w/w sediment. The disc-stack centrifuge for pilot scale centrifugation, on the other hand, could achieve up to 20% w/w sediment when feeding samples with 1-5% w/w at 1 L/min, 9,000 rpm. Wetter sediment at a pilot-scale did not lead to higher contaminants in the finished product, allowing the process scale up. Before spray drying, the beta-glucan recovery yield in laboratory- and pilot-scale were 21% and 10%, respectively. The major loss during pilot-scale production was, indeed, due to disc-stack centrifuge. It is important that the flow rate used has to be compromised between the processing time required and % solid recovery. Flow duration, however, did not influence the recovery until the point that the bowl was completely full.
DOI: 10.14455/tsb.res.2019.13
title: Comparison of Quantitative PCR and Droplet Digital PCR for Effective Hepatitis B Covalently Closed Circular DNA Measurement

description: Chronic hepatitis B virus (HBV) infection is caused by the persistence of viral covalently closed circular DNA (cccDNA) in infected hepatocytes. Targeting cccDNA is a novel approach for chronic HBV infection treatments. Since the number of cccDNA presented in a hepatocyte is very low, the precise and sensitive technique has to be developed in order to detect and quantify cccDNA. Even though quantitative polymerase chain reaction (PCR) was used for almost 3 decades by fluorescent dyes or probes to detect the amplification of DNA target, this technique only provides a relative quantification that is dependent with calibration curve. This limitation was resolved by developing of droplet digital PCR (ddPCR). ddPCR offers an absolute quantification that calculated based on Poisson algorithm. Furthermore, it is advantageous by high sensitivity and reproducibility to detect low amplicon template with extreme precision. This study presents the successful validation of ddPCR for applying to estimate cccDNA copy number. By using known concentration of plasmid, ddPCR showed the lowest detection limit around 0.1 copies per 1X reaction volume. The accuracy to quantify cccDNA target was compared between theoretical and practical value and determined the correlation by R-squared close to 1. From our results, we exhibited that ddPCR should be a platform for quantifying cccDNA and applied in subsequent researches in order to evaluate the precise cccDNA number after cccDNA-targeting treatments