DOI: 10.5061/dryad.3n5tb2rs4
title: Data from: Genetic independence of naturally correlated variation in resistance to endemic and novel pathogens

description: With anther-smut disease from wild populations, we used selection
 experiments and genetic analyses to show that, although positively
 correlated, novel and endemic-pathogen resistances are genetically
 independent, with contrasting modes of inheritance.  We
 demonstrate that polymorphic resistance to a newly introduced disease is
 genetically determined and not an extension of defenses against the
 related endemic pathogen, challenging the conventional view of nonhost
 resistance.
description: Data were collected from greenhouse-grown plants of
 <em>Silene vulgaris</em>, assessing their infection status
 after inoculation with the fungal pathogen, Microbotryum sp.  Data are
 presented as counts of healthy or diseased plants, without other
 processing.
description: # Independence of natural variation in resistance to endemic and novel
 pathogens.
 [https://doi.org/10.5061/dryad.3n5tb2rs4](https://doi.org/10.5061/dryad.3n5tb2rs4) The submitted data were generated from inoculations experiments with *Silene vulgaris* across three generations (G1 to G3) of a recurrent selection experiment.  The recurrent selection approach was used to derive *S. vulgaris* lineages with specific combinations of resistances to the endemic (*Microbotryum silenes-inflatae*) and novel (*Microbotryum lychnidis-dioicae*) pathogens.  Recurrent selection involves cycles of phenotyping and recombination among selected individuals while maintaining genetic variability within the selected populations.  In this study, the recurrent selection was performed on four groups selecting for different phenotypic combinations of resistance levels to the endemic and novel pathogens, namely, resistant to both pathogens (RR), susceptible to both pathogens (SS), resistant to the endemic and susceptible to the novel pathogen (RS), and susceptible to the endemic and resistant to the novel pathogen (SR). Following this selection, crosses between the selection groups were used to assess the dominance structure of resistance. We quantified the response to selection, assessing whether resistance to the endemic and novel pathogens evolved independently, and whether the resistances exhibited different patterns of dominance structure.   These materials contain tables of data as counts of healthy and diseased plants of *Silene vulgaris* following inoculations with the fungal anther-smut pathogen, *Microbotryum* sp. They include the inoculation results across three generations (G1 to G3) of a recurrent selection experiment.  Data are also included from the assessment of dominance of the resistance trait, with a table showing the counts of healthy and diseased plants for inoculated F1 progeny of crosses between parental groups, and counts of healthy and diseased plants for the inoculated progeny of crosses within parental groups.  ## Description of the data and file structure The data are presented in tables, separately for the three generations of the selection experiment (generations G1 to G3) with the plant *Silene vulgaris*.  Names for the different families, which are sets of offspring from a particular maternal parent plant, used for the experiment are given, along with indication of their parents’ family names from the prior generation.  Also indicates are the category names of groups selected for different phenotypic combinations of resistance levels to the endemic and novel pathogens, namely, resistant to both pathogens (RR), susceptible to both pathogens (SS), resistant to the endemic and susceptible to the novel pathogen (RS), and susceptible to the endemic and resistant to the novel pathogen (SR). The results of inoculation are shown in separate tables for in each generation of recurrent selection and the result of the dominance assay in separate XLS file.  Count data are shown for results of inoculation with either the endemic pathogen (*Microbotryum silenes-inflatae*) or the novel pathogen (*Microbotryum lychnidis-dioicae*) in separate columns in terms the number of plants that remained healthy or became diseased.  Also, the infection rates, as the proportion diseased (i.e. numbers diseased divided by the sum of the numbers healthy and numbers diseased, are also given.  For the results from the assay for dominance of the disease resistance, similar results are shown for offspring families, presented as numbers of diseased or healthy plants and the calculated proportion diseased.  Particular crosses between the groups selected for different phenotypic combinations of resistances are indicated by the “x” indicating the cross, or “within group” indicating they were not the result of crossing between the groups.  The four files corresponding these data are named “Table 1 G1,” “Table 2 G2,” “Table 3 G3,” and “Table 4 Dominance Assay,” following by the project title “Independence of natural variation in resistance to endemic and novel pathogens. Hood et al 2024.xlsx.”