cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.4121/feb7423b-8194-4d99-89d8-593023e06473
title: Data underlying chapter 3 of PhD thesis: Building the genome of a minimal synthetic cell

description: This dataset belongs to the PhD thesis of Céline Cleij titled "Building the genome of a minimal synthetic cell".Specifically, the dataset belongs to Chapter 3 titled "Synthetic chromosome assembly in yeast for cell-free protein synthesis".<br>Authors: Céline Cleij, Pascale Daran-Lapujade, Christophe DanelonDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology;Department of Biotechnology, Delft University of Technology;Toulouse Biotechnology Institute (TBI), Université de Toulouse, CNRS, INRAE, INSA<br>Corresponding authors: Pascale Daran-Lapujade and Christophe Danelon<br>Contact information: p.a.s.daran-lapujade@tudelft.nl and danelon@insa-toulouse.fr<br><br>*** General introduction ***This dataset contains data collected during experiments as part of Céline Cleij's PhD project. The data was collected from 2021-2025.<br><br>*** Methodological information ***Raw Nanopore sequencing reads (fastq) were obtained in-house using MinION technology (Oxford Nanopore Technologies, Oxford, UK).Consensus SynChrs sequences (GenBank) were obtained after de novo assembly of the processed Nanopore sequencing reads using Flye or Canu. If necessary, a consensus SynChr sequence was assembled in SnapGene using information from the Flye and Canu assemblies and raw reads.Designed SynChr sequences (GenBank) were prepared using SnapGene, using the plasmid maps of the sequenced template plasmids and the designed primer sequences.Tables S3.1-S3.17 were prepared in Excel.<br>All data processing and analysis steps are described in detail in the Methods section of the publication.<br><br>*** Organization of the dataset ***The data is grouped into four files:<br>i) Zip file "Raw Nanopore sequencing reads"Files are named after the yeast strain from which total DNA was extracted. For IMF51 and IMF54, raw reads of the second sequencing run are deposited.<br>ii) Zip file "Consensus SynChr sequences"Files are named after the yeast strain from which total DNA was extracted, and after the SynChr version (SynChr_control or SynChr_PURE) which was assembled in this strain.<br>iii) Zip file "Designed SynChr sequences"Files are named after the SynChr version (SynChr_control, SynChr_PURE, SynChr_control_2mu, SynChr_PURE_2mu).<br>iv) Excel file "Tables S3.1-S3.17"This Excel file contains the supplementary tables S3.1 to S3.17, which contain information about all used strains, synthetic chromosomes, plasmids, primers and SHRs used in this study.
DOI: 10.4121/feb7423b-8194-4d99-89d8-593023e06473.v2
title: Data underlying chapter 3 of PhD thesis: Building the genome of a minimal synthetic cell

description: This dataset belongs to the PhD thesis of Céline Cleij titled "Building the genome of a minimal synthetic cell".Specifically, the dataset belongs to Chapter 3 titled "Synthetic chromosome assembly in yeast for cell-free protein synthesis".<br>Authors: Céline Cleij, Pascale Daran-Lapujade, Christophe DanelonDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology;Department of Biotechnology, Delft University of Technology;Toulouse Biotechnology Institute (TBI), Université de Toulouse, CNRS, INRAE, INSA<br>Corresponding authors: Pascale Daran-Lapujade and Christophe Danelon<br>Contact information: p.a.s.daran-lapujade@tudelft.nl and danelon@insa-toulouse.fr<br><br>*** General introduction ***This dataset contains data collected during experiments as part of Céline Cleij's PhD project. The data was collected from 2021-2025.<br><br>*** Methodological information ***Raw Nanopore sequencing reads (fastq) were obtained in-house using MinION technology (Oxford Nanopore Technologies, Oxford, UK).Consensus SynChrs sequences (GenBank) were obtained after de novo assembly of the processed Nanopore sequencing reads using Flye or Canu. If necessary, a consensus SynChr sequence was assembled in SnapGene using information from the Flye and Canu assemblies and raw reads.Designed SynChr sequences (GenBank) were prepared using SnapGene, using the plasmid maps of the sequenced template plasmids and the designed primer sequences.Tables S3.1-S3.17 were prepared in Excel.<br>All data processing and analysis steps are described in detail in the Methods section of the publication.<br><br>*** Organization of the dataset ***The data is grouped into four files:<br>i) Zip file "Raw Nanopore sequencing reads"Files are named after the yeast strain from which total DNA was extracted. For IMF51 and IMF54, raw reads of the second sequencing run are deposited.<br>ii) Zip file "Consensus SynChr sequences"Files are named after the yeast strain from which total DNA was extracted, and after the SynChr version (SynChr_control or SynChr_PURE) which was assembled in this strain.<br>iii) Zip file "Designed SynChr sequences"Files are named after the SynChr version (SynChr_control, SynChr_PURE, SynChr_control_2mu, SynChr_PURE_2mu).<br>iv) Excel file "Tables S3.1-S3.17"This Excel file contains the supplementary tables S3.1 to S3.17, which contain information about all used strains, synthetic chromosomes, plasmids, primers and SHRs used in this study.
DOI: 10.22002/nc8j6-jrh06
title: CLEM of TRIM5a-YFP in HeLa cells

description: HeLa cells expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of red 200 nm beads 1/300. 10 nm gold. Manual blotting. Cells grown on grids for 2 days and look vesiculated.
description: <strong>Tilt Series Date:</strong> 2014-06-17</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> Hela cells expressing TRIM5a-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 136.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD and Raptor</p>
<strong>Collaborators and Roles:</strong> Tom Hope and Wes Sundquist</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Gold London finder grids glow discharged twice. Concentration of red 200 nm beads 1/300 on grid. 10 nm gold. Manual blotting. Cells grown on grids for 2 days and look vesiculated.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-06-17-1/rawdata/</p>tomography10.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-06-17-1/rawdata/tomography10.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography10_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-06-17-1/3dimage_43971/tomography10_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9jfve-3gg07
title: Eco 031910 MP49

description: Escherichia coli, 10mar24a_MP_49II_037,
description: <strong>Tilt Series Date:</strong> 2010-03-24</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 19.1 Martin</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-03-24-21/rawdata/</p>10mar24a_MP_49II_037.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-03-24-21/rawdata/10mar24a_MP_49II_037.mrc"
        > <i class="download icon"></i> Download </a></p>

        10mar24a_MP_49II_037_full.rec,  Reconstruction (Pixel Size 1.9 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2010-03-24-21/3dimage_14699/10mar24a_MP_49II_037_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.y9g837
title: Occurrence Download

description: A dataset containing 3987 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Phytophthora infestans"
 ]
}

 The dataset includes 3987 records from 146 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047557-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/24z99-kt136
title: Pfl 052709 627

description: Prosthecobacter fluviatilis, 09may28a_627_052709_008, filaments,
description: <strong>Tilt Series Date:</strong> 2009-05-28</p>
<strong>Data Taken By:</strong> Martin Pilhofer</p>
<strong>Species / Specimen:</strong> Prosthecobacter fluviatilis</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> cultured by Martin Pilhofer; data collected by Martin Pilhofer; processed by Martin Pilhofer</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10nm gold, 100% humidity, blotting 1/-2/2/0/1/4/200</p>
  mpa2009-05-28-7_slicer9727.jpg: Prosthecobacter fluviatilis .  unknown filament mpa2009-05-28-7_slicer10421.jpg: filament near membrane .  Prosthecobacter fluviatilis .  snapshot by Megan
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-05-28-7/rawdata/</p>09may28a_627_052709_008.mrc,  Tilt Series (Pixel Size 0.95 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-05-28-7/rawdata/09may28a_627_052709_008.mrc"
        > <i class="download icon"></i> Download </a></p>

        09may28a_627_052709_008_part.rec,  Reconstruction (Pixel Size 1.9 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mpa2009-05-28-7/3dimage_13829/09may28a_627_052709_008_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/rsqw6-8m298
title: Hyphomonas session11 34KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-03-29</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media...</p>
<strong>Sample Preparation:</strong> C-flat grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-32/rawdata/</p>09mar29a_bx608a_034.mrc,  Tilt Series (Pixel Size 0.632 nm), 553.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-32/rawdata/09mar29a_bx608a_034.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar29a_bx608a_034_part.rec,  Reconstruction (Pixel Size 1.264 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-03-29-32/3dimage_7336/09mar29a_bx608a_034_part.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gj96b-1rd80
title: HeLa CHMP4A+VPS4DN

description: HeLa cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2011-02-08</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with polyfect</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-02-08-2/rawdata/</p>11feb08b_C50-4c_002.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-02-08-2/rawdata/11feb08b_C50-4c_002.st"
        > <i class="download icon"></i> Download </a></p>

        11feb08b_C50-4c_002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-02-08-2/3dimage_68013/11feb08b_C50-4c_002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c3zeg-d5j04
title: Vibrio LB + Ca-HEPES

description: Vibrio cholerae, 37°C, LB + Ca-HEPES
description: <strong>Tilt Series Date:</strong> 2014-01-03</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> MKW1383 grown overnight in LB + Ca-HEPES at 37°C</p>
  aba2014-01-03-6_slicer6926.jpg: flagellar motor aba2014-01-03-6_slicer6930.jpg: chemoreceptor aba2014-01-03-6_slicer6937.jpg: flagellar motor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-03-6/rawdata/</p>VibrioBdellomediumJan0006.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-03-6/rawdata/VibrioBdellomediumJan0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        VibrioBdellomediumJan0006_part121_2.rec,  Reconstruction (Pixel Size 1.28 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2014-01-03-6/3dimage_37450/VibrioBdellomediumJan0006_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pyfer-avr39
title: Agrobacterium tumefaciens C1

description: Wild type, no T4SS
description: <strong>Tilt Series Date:</strong> 2014-02-01</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
  etl2014-02-01-10_slicer7004.jpg: filament etl2014-02-01-10_slicer7018.jpg: unknown filament etl2014-02-01-10_slicer7019.jpg: unknown filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-01-10/rawdata/</p>c10010.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-01-10/rawdata/c10010.mrc"
        > <i class="download icon"></i> Download </a></p>

        c10010.rec,  Reconstruction (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-01-10/3dimage_49216/c10010.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/95gg1-4ae52
title: A22-treatment methanol control

description: Raw data files of A22-treatment methanol control
description: <strong>Tilt Series Date:</strong> 2009-07-30</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> Cell culture and treatment were done by Zhuo Li. Data were collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Exponential-phase NA1000 cells were treated with mathanol for 2 hours. </p>
<strong>Sample Preparation:</strong> 10 nm</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-07-30-26/rawdata/</p>09jul30a_Methanol_026.mrc,  Tilt Series (Pixel Size 1.2 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-07-30-26/rawdata/09jul30a_Methanol_026.mrc"
        > <i class="download icon"></i> Download </a></p>

        09jul30a_Methanol_026_full.rec,  Reconstruction (Pixel Size 2.4 nm), 367.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-07-30-26/3dimage_6036/09jul30a_Methanol_026_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yeqja-wvv25
title: Hyphomonas EB session14 27.5KX

description: high-order DNA, H-NS, nucleoid, asymmetric cell division
description: <strong>Tilt Series Date:</strong> 2009-05-22</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Hyphomonas neptunium</p>
<strong>Strain:</strong> ATCC 15444</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-62.0°, 62.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Cultured by Jian; data collected by Jian; processed by Jian</p>
<strong>Purification / Growth Conditions / Treatment:</strong> exponential phase in PYE media, treated with EB...</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-2/rawdata/</p>09may22a_bx200d_002.mrc,  Tilt Series (Pixel Size 0.774 nm), 520.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-2/rawdata/09may22a_bx200d_002.mrc"
        > <i class="download icon"></i> Download </a></p>

        09may22a_bx200d_002_1k.rec,  Reconstruction (Pixel Size 1.548 nm), 91.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2009-05-22-2/3dimage_7588/09may22a_bx200d_002_1k.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/22701
title: Chandra X-ray Observatory ObsId 22701

description: Measure Si K and Fe L edges in 4U 1636-53
DOI: 10.34740/kaggle/dsv/12879625
title: Peer-to-Peer Boliviano (BOB) Exchange Data
DOI: 10.22002/mdjwf-sf021
title: Agrobacterium tumefaciens

description: VG3101 with Ti plasmid
description: <strong>Tilt Series Date:</strong> 2013-11-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> AutoPatch</p>
<strong>Collaborators and Roles:</strong> Elliot Meyerwitz</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Growth in LB + antibiotics</p>
<strong>Sample Preparation:</strong> Vitrobot blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-31/rawdata/</p>atlas40001.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-31/rawdata/atlas40001.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas40001.rec,  Reconstruction (Pixel Size 1.6 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-11-11-31/3dimage_34250/atlas40001.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.25574/24462
title: Chandra X-ray Observatory ObsId 24462

description: Density diagnostics of outflows in MR 2251-178
DOI: 10.15468/dl.4a8gjv
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Polyplectron schleiermacheri",
 "Country is China",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047558-250811113504898/datasets/export for details.
DOI: 10.25574/24469
title: Chandra X-ray Observatory ObsId 24469

description: Density diagnostics of outflows in MR 2251-178