DOI: 10.5281/zenodo.14979471
title: BIO407 Group6 CLSM images of DFX treated cells

description: ## Study:

This data is part of the practical course BIO407 2025 at the University of Zurich, titled 'Advanced Microscopy: From preparation to data and visualization'.

The effects of Deferasirox (DFX) on mitochondria morphology were examined using different microscopy techniques.

 

Course: BIO407

 

group-number:

-1: -Jessica Goetschi

-Alexa Fry

-Luca Vogt

 

## Study Component:

Job: Confocal microscopy

Task: 8 'Imaging Mitochondira in DFX-treated Cells'

Using confocal microscopy, images of DFX-treated and untreated cells were acquired.

 

## Biosample:

COS-7 cells

 

## Specimen:

Four different conditions:

-Control 1: No treatment

-Control 2: 5h DMSO treatment

-Condition 1: 3h 200uM DFX treatment

-Condition 2: 5h 200uM DFX treatment

 

Cells were fixed with 4% PFA, 0.05% GTA, 0.5% Saponin in PHEM.

Cells were labeled with DAPI, TOMM20-Alexa488, Tubulin-Alexa568, SiR-Actin.

 

## Image Acquisition:

 

Images were acquired using a Leica DMI6000B, Model SP8.

Acquired channels:

Channel 1: DAPI, 405nm excitation, 412nm-481nm emission, PMT Detector 

Channel 2: Alexa488-TOMM20, 488nm excitation, 497nm-547nm emission, HyD Detector

Channel 3: Alexa568-Tubulin, 561nm excitation, 570nm-625nm emission, HyD Detector

Channel 4: SiR-actin, 633nm excitation, 650nm-721nm emission, HyD

Objectives used:

- Objective 1: 10x HC PL FLUOTAR dry objective, 0.3NA

- Objective 2: 20x HC PL APO CS2 imm(oil) objective, 0.75NA

- Objective 3: 63x HC PL APO CS2 oil objective, 1.4NA

 

## Image Data:

Project Name Convention: [Blockcourse]_[Group]_[Microscope]_[Raw/Processed Image]_[DDMMYYYY]_[Sample]_[Task].lif

 

BIO407_G6_SP8_Falcon_Raw_27022025_S6.1_Task1.lif

- 10xAIR_vis_focused_task1.1 focused using channel 2 with objecive 10xAIR

- 20xOIL_task1.2

- 63xOIL_task1.3

- 63xOIL_task1.3_v2

 

BIO407_G6_SP8_Falcon_Raw_27022025_S6.1_Task2.lif

- 63xOIL_sim_task2.1 simultaneous image acquisition

- 63xOIL_seq_task2.2

BIO407_G6_SP8_Falcon_Raw_28022025_S6.1_Task3.lif

- 63xOIL_slowscan5180ns_task3.1 5180nspixel dwell time

- 63xOIL_fastscan800ns_task3.2

- 63xOIL_ultrafastscan1000ns_pixelsize12nm_task3.3

 

BIO407_G6_SP8_Falcon_Raw_28022025_S6.1_Task4.lif

- 63xOIL_x%_task4 Different laser intensities in % 

 

BIO407_G6_SP8_Falcon_Raw_28022025_S6.1_Task5.lif

- 63xOIL_1xAcc1xAv_CC3_task5.1 Color channel 3 & 1x Line Accumulation and 1x Line Averaging

- 63xOIL_1xAcc2xAv_CC3_task5.2

- 63xOIL_1xAcc4xAv_CC3_task5.3

- 63xOIL_1xAcc8xAv_CC3_task5.3

- 63xOIL_1xAcc1xAv_CC2_task5.1

- 63xOIL_1xAcc2xAv_CC2_task5.2

- 63xOIL_1xAcc4xAv_CC2_task5.3

- 63xOIL_2xAcc1xAv_CC2_task5.2

- 63xOIL_4xAcc1xAv_CC2_task5.3

 

BIO407_G6_SP8_Falcon_Raw_28022025_S6.1_Task6.lif

- 63xOIL_3D_1AU_task6.2_saturated 1AU pinhole diameter

- 63xOIL_3D_1AU_task6.2

- 63xOIL_3D_0.3AU_task6.1_saturated

- 63xOIL_3D_5AU_task6.3_saturated

 

BIO407_G6_SP8_Falcon_Raw_27022025_S6.1_Task7.lif

- 63xOIL_3D_Nyquist_samplingZ_task7.2 Sampling in Z direction

- 63xOIL_3D_under_samplingZ_task7.1

- 63xOIL_3D_over_samplingZ_task7.3

- 63xOIL_3D_under_samplingXYZ_task7.1

 

BIO407_G6_SP8_Falcon_Raw_28022025_S6.1_Task8.lif

- 63xOIL_3D_C2_task8 channel 2 of sample 6.1

- 63xOIL_3D_C3_task8

- 63xOIL_3D_C6_task8