DOI: 10.5061/dryad.5h1q1
title: Data from: The specific and exclusive microbiome of the deep-sea bone-eating snail, Rubyspira osteovora

description: Rubyspira osteovora is an unusual deep-sea snail from Monterey Canyon,
 California. This group has only been found on decomposing whales and is
 thought to use bone as a novel source of nutrition. This study
 characterized the gut microbiome of R. osteovora, compared to the
 surrounding environment, as well as to other deep-sea snails with more
 typical diets. Analysis of 16S rRNA gene sequences revealed that R.
 osteovora digestive tissues host a much lower bacterial diversity (average
 Shannon index of 1.9; n = 12), compared to environmental samples (average
 Shannon index of 4.4; n = 2) and are dominated by two bacterial genera:
 Mycoplasma and Psychromonas (comprising up to 56% and 42% average total
 recovered sequences, respectively). These two bacteria, along with
 Psychrilyobacter sp. (∼16% average recovered sequences), accounted for
 between 43% and 92% of the total recovered sequences in individual snail
 digestive systems, with other OTUs present at much lower proportions. The
 relative abundance of these three groups remained similar over 6 years of
 sampling (collection date was not shown to be a significant predictor of
 community structure), suggesting a long-term association. Furthermore,
 these bacterial genera were either not present (Mycoplasma and
 Psychromonas) or at very low abundance (&lt;0.04% for
 Psychrilyobacter), in environmental samples and other deep-sea gastropods,
 supporting the uniqueness of the R. osteovora gut microbiome.
description: Snail_data_OTUs_4_DryadRuby-PC44_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204D-Int_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby-PC41bone_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204C-Fec_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204B-St_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204A-St_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204A-DG_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204D-DG_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby769B-St_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby769B-Int_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby769A-St_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby769A-Int_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby204C-DG_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby1162A-St_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersRuby1162A-Int_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersPhym751-int_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersBathym2-DG_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primersBathym1-Int_seqsRaw 16S rRNA reads, from Illumina-based sequencing of V4 region using 515F/806R primers