DOI: 10.22002/zyr33-nh547
title: NR1769pRP4 conj NR1769 phage MS2

description: Skinny Ecoli NR1769 with the conjugation plasmid pRP4 conjugating with NR1769
description: <strong>Tilt Series Date:</strong> 2014-11-26</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> NR1769</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Christian Baron</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown to log phase and mixed in 1:1 ratio on 2% agar plate and left to conjugate for 30min. Phage MS2 was added to visualize any F pili</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-11-26-7/rawdata/</p>5plus7_ms20007.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
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        5plus7_ms20007.rec,  Reconstruction (Pixel Size 0.0 nm), 711.2 MB  
        
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        slicer000.tif,  2.8 MB  
        
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        slicer001.tif,  2.8 MB  
        
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        slicer002.tif,  2.8 MB  
        
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        slicer003.tif,  2.8 MB  
        
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        slicer004.tif,  2.8 MB  
        
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        slicer005.tif,  2.8 MB  
        
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