cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21115039
title: TableS3_statistical.results.xlsx from Evolution of competitive traits changes species diversity in a natural field

description: Studying the interaction between evolutionary and ecological processes (i.e. eco-evolutionary dynamics) has great potential to improve our understanding of biological processes such as species interactions, community assembly and ecosystem functions. However, most experimental studies have been conducted under controlled laboratory or mesocosm conditions, and the importance of these interactions in natural field communities has not been evaluated. In this study, we focused on the contemporary divergence of a competitive trait (the height–width ratio) of an annual grass <i>Eleusine indica</i> between urban and farmland populations and investigated how trait evolution affects ecological processes by transplanting <i>E. indica</i> individuals from lineages with different trait values into semi-natural grassland. The competitive trait of the transplanted individuals not only affected their own growth and fitness, but also affected the vegetative growth of the competing species and the species diversity. These results indicate that the evolution of competitive traits, even in a single species, can influence the community species diversity through changes in interspecific interactions. Eco-evolutionary interactions therefore play a crucial role in natural field environments. Our results suggest that understanding intraspecific variation in competitive traits driven by rapid evolution is essential for understanding interspecific competitive interactions, community assembly and species diversity.
DOI: 10.6084/m9.figshare.18866266.v2
title: Total allele count distribution (TAC curves) improves number of contributor estimation for complex DNA mixtures

description: As the forensic community is transitioning to probabilistic genotyping and the use of likelihood ratios to assign probative weight to DNA mixtures, the assessment of the number of contributors (NOC) needs to be more robust for mixture interpretation. However, NOC assessment can be challenging for low-template and/or high order mixtures. Here, we present a quick and easy-to-use tool to help with NOC estimation: total allele count curves (TAC curves). TAC curves for two to seven contributors were generated using sets of 20,000 in silico mixtures, for five populations (African American, Caucasian, Asian, Apache and Native Alaska) and for commonly used commercial STR kits (GlobalFiler<sup>TM</sup>, PowerPlex® Fusion, PowerPlex® ESX 17 and Identifiler<sup>TM</sup>). To assess the performance of TAC curves, the NOC was evaluated for 80 mixtures, with and without use of the curves. Results show that TAC curves allow for a better NOC assessment as correct evaluations rose from 10% when using maximal allele count (MAC) to 65% when also using TAC for four to six contributor mixtures. Supplemental data for this article is available online at http://dx.doi.org/10.1080/00085030.2022.2028359 .
DOI: 10.6084/m9.figshare.18866266
title: Total allele count distribution (TAC curves) improves number of contributor estimation for complex DNA mixtures

description: As the forensic community is transitioning to probabilistic genotyping and the use of likelihood ratios to assign probative weight to DNA mixtures, the assessment of the number of contributors (NOC) needs to be more robust for mixture interpretation. However, NOC assessment can be challenging for low-template and/or high order mixtures. Here, we present a quick and easy-to-use tool to help with NOC estimation: total allele count curves (TAC curves). TAC curves for two to seven contributors were generated using sets of 20,000 in silico mixtures, for five populations (African American, Caucasian, Asian, Apache and Native Alaska) and for commonly used commercial STR kits (GlobalFiler<sup>TM</sup>, PowerPlex® Fusion, PowerPlex® ESX 17 and Identifiler<sup>TM</sup>). To assess the performance of TAC curves, the NOC was evaluated for 80 mixtures, with and without use of the curves. Results show that TAC curves allow for a better NOC assessment as correct evaluations rose from 10% when using maximal allele count (MAC) to 65% when also using TAC for four to six contributor mixtures. Supplemental data for this article is available online at http://dx.doi.org/10.1080/00085030.2022.2028359 .
DOI: 10.6084/m9.figshare.18866266.v1
title: Total allele count distribution (TAC curves) improves number of contributor estimation for complex DNA mixtures

description: As the forensic community is transitioning to probabilistic genotyping and the use of likelihood ratios to assign probative weight to DNA mixtures, the assessment of the number of contributors (NOC) needs to be more robust for mixture interpretation. However, NOC assessment can be challenging for low-template and/or high order mixtures. Here, we present a quick and easy-to-use tool to help with NOC estimation: total allele count curves (TAC curves). TAC curves for two to seven contributors were generated using sets of 20,000 in silico mixtures, for five populations (African American, Caucasian, Asian, Apache and Native Alaska) and for commonly used commercial STR kits (GlobalFiler<sup>TM</sup>, PowerPlex® Fusion, PowerPlex® ESX 17 and Identifiler<sup>TM</sup>). To assess the performance of TAC curves, the NOC was evaluated for 80 mixtures, with and without use of the curves. Results show that TAC curves allow for a better NOC assessment as correct evaluations rose from 10% when using maximal allele count (MAC) to 65% when also using TAC for four to six contributor mixtures. Supplemental data for this article is available online at http://dx.doi.org/10.1080/00085030.2022.2028359 .
DOI: 10.15151/esrf-es-874760332
title: IBS BAG

description: The IBS BAG includes in this proposal IBS teams and external groups from iRTSV/CEA (Grenoble), CEA-Cadarache (St Paul lez Durance) and IRBA (Brétigny-sur-Orge). We study structure-function relationships of proteins emphasizing in human health, biotechnology and methodology. Most of the projects are a continuation of the MX-2329 proposal and can be grouped in different topics as follows: host-pathogen interaction, new drug targets, fundamental cellular mechanisms, membrane proteins, intrinsically disordered proteins, biotechnological applications and methodology. Some of these projects use integrative approaches (X-ray crystallography, SAXS, NMR (and cryo-EM), together with other biophysical studies) with the aim to fully understand the complexity of the biological macromolecular systems on study. Some teams are dedicated to serial crystallography and its developments, for which the ESRF-EBS has become the perfect tool.
DOI: 10.15151/esrf-es-874809193
title: Structural biology at Umea University

description: The BAG consists out of 7 groups that are heavily involved in macromolecular crystallography, as well as other structural biology techniques, at Umeå University, Sweden. We work on a diverse set of biological questions, ranging from understanding the structure and function of Gram-positive Type 4 Secretion Systems, mobile genetic elements in Staphylococcus aureus, DNA and RNA polymerases, effector protein in Type 6 Secretion Systems and transcriptional regulators.
DOI: 10.15151/esrf-es-894562551
title: UK Midlands BAG

description: The UK Midlands BAG consists of groups located at the Universities of Birmingham, Leicester, Warwick, Nottingham, Keele, and Wolverhampton. We represent a diverse spectrum of research interests, from microbiology, cancer biology, blood disorders, immunology to fundamental biological processes such as membrane transport, DNA repair and mechanisms of enzyme catalysis. Our groups use X-ray crystallography in conjunction with other structural biology, biophysical and biochemical techniques to elucidate molecular mechanisms of protein-protein and protein-ligand interactions. Our cooperation in this block allocation group is mirrored by the consortium of institutions that are stakeholders in the Midlands Regional CryoEM Facility at the University of Leicester.
DOI: 10.15151/esrf-es-924508468
title: Single crystal XRD characterization of FexGa1-x & GID characterization of a monolith Al₂O₃ + Pt

description: We are interested in solving the crystal structure of a phase observed when liquid gallium is in contact with NaOH solution. Currently, we have EDX, STEM, SAED, EDS, SEM, and EBSD data which suggests that the hexagonal crystals that form are novel and have never been reported upon in previous literature studies. We are hoping to acquire the unit cell for the synthesized FexGa1-x particles and collect full sphere data to sufficiently high resolution so we can identify the structure ex-situ. Previous attempts to collect such data on single crystal XRD instruments have been unsuccessful due to the particles small size (hexagonal with ≈ 10 µm side length) giving limited diffraction peaks with low signal to noise. In the second part we want to characterize a monolith of alumina coated with low coverage of platinum, in order to see if this materials is suited for a liquid organic hydrogen carrier (LOHC) reactor.
DOI: 10.6084/m9.figshare.14137958
title: “Do what you can with a happy heart”: a longitudinal study of patient and family members’ lived experiences of physical activity post-myocardial infarction

description: Physical activity (PA) post-myocardial infarction (MI) can reduce risk of reoccurrence and mortality. Yet uptake of PA through cardiac rehabilitation (CR) is poor, and little is known about the long-term PA behaviour of cardiac patients. This study aimed to explore the lived experiences of patients’ engagement with PA post-MI, together with the experiences of their family. Longitudinal interviews with six family-dyads were conducted which drew on interpretative phenomenology to understand the lived experiences of PA for post-MI patients and family members. Participants described a journey that involved leaving “normality” (doing what PA they wanted and when) and transitioning to a new “normality” (being active within post-MI parameters). This journey was expressed through feelings of “I can’t do what I was doing before,” “finding my way,” and “accepting this way”. The role of family within the patient’s journey was complex, with PA identity, beliefs, and fear of MI re-occurrence influencing PA support both positively and negatively. PA engagement post-MI is a dynamic and interactive process within which the family can have an important influence.IMPLICATIONS FOR REHABILITATIONThe process of (re-)engaging in physical activity post-myocardial infarction involves a complex journey towards acceptance of a new “normality”.Family play an important role in regulating and supporting patients’ physical activity during the rehabilitation process.Promoting positive health beliefs and helping families understand what, how and when patients should be physically active may optimise the rehabilitation journey for post-myocardial infarction patients. The process of (re-)engaging in physical activity post-myocardial infarction involves a complex journey towards acceptance of a new “normality”. Family play an important role in regulating and supporting patients’ physical activity during the rehabilitation process. Promoting positive health beliefs and helping families understand what, how and when patients should be physically active may optimise the rehabilitation journey for post-myocardial infarction patients.
DOI: 10.6084/m9.figshare.13589826
title: The role of theory to develop and evaluate a toolkit to increase clinical measurement and interpretation of walking speed and distance in adults post-stroke

description: The iWalk study showed that 10-meter walk test (10mWT) and 6-minute walk test (6MWT) administration post-stroke increased among physical therapists (PTs) following introduction of a toolkit comprising an educational guide, mobile app, and video. We describe the use of theory guiding toolkit development and a process evaluation. We used the knowledge-to-action framework to identify research steps; and a guideline implementability framework, self-efficacy theory, and the transtheoretical model to design and evaluate the toolkit and implementation process (three learning sessions). In a before-and-after study, 37 of the 49 participating PTs completed online questionnaires to evaluate engagement with learning sessions, and rate self-efficacy to perform recommended practices pre- and post-intervention. Thirty-three PTs and 7 professional leaders participated in post-intervention focus groups and interviews, respectively. All sites conducted learning sessions; attendance was 50-78%. Self-efficacy ratings for recommended practices increased and were significant for the 10mWT (<i>p</i> ≤ 0.004). Qualitative findings highlighted that theory-based toolkit features and implementation strategies likely facilitated engagement with toolkit components, contributing to observed improvements in PTs’ knowledge, attitudes, skill, self-efficacy, and clinical practice. The approach may help to inform toolkit development to advance other rehabilitation practices of similar complexity.Implications for RehabilitationToolkits are an emerging knowledge translation intervention used to support widespread implementation of clinical practice guideline recommendations.Although experts recommend using theory to inform the development of knowledge translation interventions, there is little guidance on a suitable approach.This study describes an approach to using theories, models and frameworks to design a toolkit and implementation strategy, and a process evaluation of toolkit implementation.Theory-based features of the toolkit and implementation strategy may have facilitated toolkit implementation and practice change to increase clinical measurement and interpretation of walking speed and distance in adults post-stroke. Toolkits are an emerging knowledge translation intervention used to support widespread implementation of clinical practice guideline recommendations. Although experts recommend using theory to inform the development of knowledge translation interventions, there is little guidance on a suitable approach. This study describes an approach to using theories, models and frameworks to design a toolkit and implementation strategy, and a process evaluation of toolkit implementation. Theory-based features of the toolkit and implementation strategy may have facilitated toolkit implementation and practice change to increase clinical measurement and interpretation of walking speed and distance in adults post-stroke.
DOI: 10.6084/m9.figshare.13589826.v1
title: The role of theory to develop and evaluate a toolkit to increase clinical measurement and interpretation of walking speed and distance in adults post-stroke

description: The iWalk study showed that 10-meter walk test (10mWT) and 6-minute walk test (6MWT) administration post-stroke increased among physical therapists (PTs) following introduction of a toolkit comprising an educational guide, mobile app, and video. We describe the use of theory guiding toolkit development and a process evaluation. We used the knowledge-to-action framework to identify research steps; and a guideline implementability framework, self-efficacy theory, and the transtheoretical model to design and evaluate the toolkit and implementation process (three learning sessions). In a before-and-after study, 37 of the 49 participating PTs completed online questionnaires to evaluate engagement with learning sessions, and rate self-efficacy to perform recommended practices pre- and post-intervention. Thirty-three PTs and 7 professional leaders participated in post-intervention focus groups and interviews, respectively. All sites conducted learning sessions; attendance was 50-78%. Self-efficacy ratings for recommended practices increased and were significant for the 10mWT (<i>p</i> ≤ 0.004). Qualitative findings highlighted that theory-based toolkit features and implementation strategies likely facilitated engagement with toolkit components, contributing to observed improvements in PTs’ knowledge, attitudes, skill, self-efficacy, and clinical practice. The approach may help to inform toolkit development to advance other rehabilitation practices of similar complexity.Implications for RehabilitationToolkits are an emerging knowledge translation intervention used to support widespread implementation of clinical practice guideline recommendations.Although experts recommend using theory to inform the development of knowledge translation interventions, there is little guidance on a suitable approach.This study describes an approach to using theories, models and frameworks to design a toolkit and implementation strategy, and a process evaluation of toolkit implementation.Theory-based features of the toolkit and implementation strategy may have facilitated toolkit implementation and practice change to increase clinical measurement and interpretation of walking speed and distance in adults post-stroke. Toolkits are an emerging knowledge translation intervention used to support widespread implementation of clinical practice guideline recommendations. Although experts recommend using theory to inform the development of knowledge translation interventions, there is little guidance on a suitable approach. This study describes an approach to using theories, models and frameworks to design a toolkit and implementation strategy, and a process evaluation of toolkit implementation. Theory-based features of the toolkit and implementation strategy may have facilitated toolkit implementation and practice change to increase clinical measurement and interpretation of walking speed and distance in adults post-stroke.
DOI: 10.6084/m9.figshare.14098548
title: Participation and engagement in family activities among girls and young women with Rett syndrome living at home with their parents – a cross-sectional study

description: To describe the extent of participation and engagement in family activities and explore variables potentially impacting on these factors in family activities among girls and young women with Rett syndrome (RTT) under the age of 21. The Child Participation in Family Activities (Child-PFA) questionnaire was sent to parents in the target group (<i>n</i> = 42). Additionally, age, number of siblings at home, ambulation level, clinical severity and level of hand function were recorded to explore possible impact. Data were analyzed using descriptive statistics, Fishers exact test and cross-tables. 23 families participated. Highest degrees of participation and engagement were seen in social and stationary family activities. <i>Indoor activities</i> were frequent and showed high levels of participation and engagement, <i>Outdoor activities</i> were infrequent and showed low levels of participation despite a high degree of engagement. <i>Routine activities</i> were frequent but showed moderate to low participation and engagement. A negative association was found between participation in watching a movie and number of siblings living at home, and positive associations between engagement and age in three family activities. Therapists working with this target group may benefit from focusing on engagement in routine activities and modification of family activities.IMPLICATIONS FOR REHABILITATIONTherapists may benefit from focusing on engagement in routine activities in the goal setting process and intervention as they occur on a daily basis, giving the opportunity for development of new skills.Therapists may benefit from focusing on assistive devices or other compensatory strategies for outdoor activities and activities that require a certain amount of hand function.Therapists may benefit from modifying the family’s activities so that they require more social and mental participation and focus on experiencing different types of sensory input e.g., sound, tactile, visual or vestibular input rather than taking part in the activity by using their hands. Therapists may benefit from focusing on engagement in routine activities in the goal setting process and intervention as they occur on a daily basis, giving the opportunity for development of new skills. Therapists may benefit from focusing on assistive devices or other compensatory strategies for outdoor activities and activities that require a certain amount of hand function. Therapists may benefit from modifying the family’s activities so that they require more social and mental participation and focus on experiencing different types of sensory input e.g., sound, tactile, visual or vestibular input rather than taking part in the activity by using their hands.
DOI: 10.6084/m9.figshare.14098548.v1
title: Participation and engagement in family activities among girls and young women with Rett syndrome living at home with their parents – a cross-sectional study

description: To describe the extent of participation and engagement in family activities and explore variables potentially impacting on these factors in family activities among girls and young women with Rett syndrome (RTT) under the age of 21. The Child Participation in Family Activities (Child-PFA) questionnaire was sent to parents in the target group (<i>n</i> = 42). Additionally, age, number of siblings at home, ambulation level, clinical severity and level of hand function were recorded to explore possible impact. Data were analyzed using descriptive statistics, Fishers exact test and cross-tables. 23 families participated. Highest degrees of participation and engagement were seen in social and stationary family activities. <i>Indoor activities</i> were frequent and showed high levels of participation and engagement, <i>Outdoor activities</i> were infrequent and showed low levels of participation despite a high degree of engagement. <i>Routine activities</i> were frequent but showed moderate to low participation and engagement. A negative association was found between participation in watching a movie and number of siblings living at home, and positive associations between engagement and age in three family activities. Therapists working with this target group may benefit from focusing on engagement in routine activities and modification of family activities.IMPLICATIONS FOR REHABILITATIONTherapists may benefit from focusing on engagement in routine activities in the goal setting process and intervention as they occur on a daily basis, giving the opportunity for development of new skills.Therapists may benefit from focusing on assistive devices or other compensatory strategies for outdoor activities and activities that require a certain amount of hand function.Therapists may benefit from modifying the family’s activities so that they require more social and mental participation and focus on experiencing different types of sensory input e.g., sound, tactile, visual or vestibular input rather than taking part in the activity by using their hands. Therapists may benefit from focusing on engagement in routine activities in the goal setting process and intervention as they occur on a daily basis, giving the opportunity for development of new skills. Therapists may benefit from focusing on assistive devices or other compensatory strategies for outdoor activities and activities that require a certain amount of hand function. Therapists may benefit from modifying the family’s activities so that they require more social and mental participation and focus on experiencing different types of sensory input e.g., sound, tactile, visual or vestibular input rather than taking part in the activity by using their hands.