cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25442/hku.16438623
title: Supporting data for “Conversion of stem cells from apical papilla into endothelial cells by small molecules and growth factors”.

description: Incorporating assembled microvascular networks into the bioengineered dental pulp constructs can significantly enhance functional blood circulation and tissue survival after transplantation. Endothelial cells (ECs), the essential cellular building blocks of vascular tissue, play an important role in the process of pre-vascularization. However, obtaining sufficient ECs from a suitable source for translational application is challenging. Dental stem cells (DSCs), which exhibit a robust proliferative ability and immunocompatibility because of their autologous origin, could be a promising alternative cell source for the derivation of endothelial lineages. Under specific culture conditions, DSCs differentiate into osteo/odontogenic, adipogenic, chondrogenic, and neurogenic cell lineages. DSCs also possess the potential to differentiate into endothelial-like cells.Recently, a new strategy has been developed to directly reprogram one cell type towards another targeted cell type using small molecule compounds modulating epigenetic status and signaling pathways. Human fibroblasts have been chemically reprogrammed into neuronal cells, Schwann cells and cardiomyocyte-like cells by different small molecules combinations. This study aimed to explore whether stem cells from apical papilla (SCAP) could be reprogrammed into endothelial cells (ECs) using the same strategy.We developed a set of small molecules and growth factors, including VPA, CHIR99021 Repsox, Forskolin, Y-27632, VEGF, BMP-4 and 8-Br-3,5-cAMP, to differentiate SCAP into endothelial linages. The expression level of endothelial specific genes and proteins were assessed by RT-PCR, western blotting, flow cytometry, and immunofluorescence after chemical induction of SCAP. The <i>in vitro</i> functions of SCAP-derived chemical-induced endothelial cells (SCAP-ECs) were evaluated by tube-like structure formation assay, acetylated low-density lipoprotein (ac-LDL) uptake and NO secretion detection. The proliferation and migration ability of SCAP-ECs were evaluated by CCK8 and Transwell assay. LPS stimulation was used to mimic inflammatory environment for demonstrating the ability of SCAP-ECs to express adhesion molecules. The in vivo Matrigel plug angiogenesis assay was performed to assess the function of SCAP-ECs in generating vascular structures using the immune-deficient mouse model.SCAP-ECs expressed up-regulated endothelial specific genes and proteins, displayed endothelial transcriptional networks, exhibited the ability to form functional tubular-like structures, uptake ac-LDL, secret NO <i>in vitro</i>, and contributed to generate blood vessels in <i>vivo</i>. The SCAP-ECs could also express adhesion molecules in the pro-inflammatory environment and have a similar migration ability like HUVECs. The results indicate that SCAP-ECs could be one promising cell source for vascular engineering and treatment of ischemic diseases.
DOI: 10.25442/hku.16438623.v1
title: Supporting data for “Conversion of stem cells from apical papilla into endothelial cells by small molecules and growth factors”.

description: Incorporating assembled microvascular networks into the bioengineered dental pulp constructs can significantly enhance functional blood circulation and tissue survival after transplantation. Endothelial cells (ECs), the essential cellular building blocks of vascular tissue, play an important role in the process of pre-vascularization. However, obtaining sufficient ECs from a suitable source for translational application is challenging. Dental stem cells (DSCs), which exhibit a robust proliferative ability and immunocompatibility because of their autologous origin, could be a promising alternative cell source for the derivation of endothelial lineages. Under specific culture conditions, DSCs differentiate into osteo/odontogenic, adipogenic, chondrogenic, and neurogenic cell lineages. DSCs also possess the potential to differentiate into endothelial-like cells.Recently, a new strategy has been developed to directly reprogram one cell type towards another targeted cell type using small molecule compounds modulating epigenetic status and signaling pathways. Human fibroblasts have been chemically reprogrammed into neuronal cells, Schwann cells and cardiomyocyte-like cells by different small molecules combinations. This study aimed to explore whether stem cells from apical papilla (SCAP) could be reprogrammed into endothelial cells (ECs) using the same strategy.We developed a set of small molecules and growth factors, including VPA, CHIR99021 Repsox, Forskolin, Y-27632, VEGF, BMP-4 and 8-Br-3,5-cAMP, to differentiate SCAP into endothelial linages. The expression level of endothelial specific genes and proteins were assessed by RT-PCR, western blotting, flow cytometry, and immunofluorescence after chemical induction of SCAP. The <i>in vitro</i> functions of SCAP-derived chemical-induced endothelial cells (SCAP-ECs) were evaluated by tube-like structure formation assay, acetylated low-density lipoprotein (ac-LDL) uptake and NO secretion detection. The proliferation and migration ability of SCAP-ECs were evaluated by CCK8 and Transwell assay. LPS stimulation was used to mimic inflammatory environment for demonstrating the ability of SCAP-ECs to express adhesion molecules. The in vivo Matrigel plug angiogenesis assay was performed to assess the function of SCAP-ECs in generating vascular structures using the immune-deficient mouse model.SCAP-ECs expressed up-regulated endothelial specific genes and proteins, displayed endothelial transcriptional networks, exhibited the ability to form functional tubular-like structures, uptake ac-LDL, secret NO <i>in vitro</i>, and contributed to generate blood vessels in <i>vivo</i>. The SCAP-ECs could also express adhesion molecules in the pro-inflammatory environment and have a similar migration ability like HUVECs. The results indicate that SCAP-ECs could be one promising cell source for vascular engineering and treatment of ischemic diseases.
DOI: 10.5517/ccdc.csd.cc22yjl5
title: CCDC 1935007: Experimental Crystal Structure Determination

description: Related Article: Mahmood Karimi Abdolmaleki, Sayandev Chatterjee, Sedigheh Barzegar, Amie E. Norton, Mark O. Bovee, Mojtaba Enayati, William B.Connick, Jeanette A.Krause, Alireza Abbaspourrad|2019|CSD Communication|||
DOI: 10.5517/ccdc.csd.cc25nhgt
title: CCDC 2015696: Experimental Crystal Structure Determination

description: Related Article: Milan Gembicky|2020|CSD Communication|||
DOI: 10.5517/ccdc.csd.cc27h8st
title: CCDC 2070266: Experimental Crystal Structure Determination

description: Related Article: Ming Li, Jun-Yang Tao, Liang-Neng Wang, Jia-Wei Li, Yue-Jin Liu, Ming-Hua Zeng|2021|J.Org.Chem.|86|11915|doi:10.1021/acs.joc.1c01329
DOI: 10.5517/ccdc.csd.cc27hymb
title: CCDC 2070912: Experimental Crystal Structure Determination

description: Related Article: Yu Ozawa, Kohei Endo, Hajime Ito|2021|J.Am.Chem.Soc.|143|13865|doi:10.1021/jacs.1c06538
DOI: 10.5517/ccdc.csd.cc27n66b
title: CCDC 2074991: Experimental Crystal Structure Determination

description: Related Article: Avishek Majumder, Nityananda Dutta, Arpan Das, Luca Carrella, Manindranath Bera|2021|Polyhedron|208|115417|doi:10.1016/j.poly.2021.115417
DOI: 10.5517/ccdc.csd.cc27n67c
title: CCDC 2074992: Experimental Crystal Structure Determination

description: Related Article: Avishek Majumder, Nityananda Dutta, Arpan Das, Luca Carrella, Manindranath Bera|2021|Polyhedron|208|115417|doi:10.1016/j.poly.2021.115417
DOI: 10.5517/ccdc.csd.cc27pj4m
title: CCDC 2076260: Experimental Crystal Structure Determination

description: Related Article: Neha Choudhary, Hayden Scheiber, Jiale Zhang, Brian O. Patrick, María de Guadalupe Jaraquemada-Peláez, Chris Orvig|2021|Inorg.Chem.|60|12855|doi:10.1021/acs.inorgchem.1c01175
DOI: 10.5517/ccdc.csd.cc27pj6p
title: CCDC 2076262: Experimental Crystal Structure Determination

description: Related Article: Neha Choudhary, Hayden Scheiber, Jiale Zhang, Brian O. Patrick, María de Guadalupe Jaraquemada-Peláez, Chris Orvig|2021|Inorg.Chem.|60|12855|doi:10.1021/acs.inorgchem.1c01175
DOI: 10.5517/ccdc.csd.cc27pj7q
title: CCDC 2076263: Experimental Crystal Structure Determination

description: Related Article: Neha Choudhary, Hayden Scheiber, Jiale Zhang, Brian O. Patrick, María de Guadalupe Jaraquemada-Peláez, Chris Orvig|2021|Inorg.Chem.|60|12855|doi:10.1021/acs.inorgchem.1c01175
DOI: 10.5517/ccdc.csd.cc27w6fs
title: CCDC 2081725: Experimental Crystal Structure Determination

description: Related Article: Nikolai Yu. Tiuftiakov, Julia O. Strelnikova, Ilya P. Filippov, Adel R. Khaidarov, Alexander F. Khlebnikov, Alexander S. Bunev, Mikhail S. Novikov, Nikolai V. Rostovskii|2021|Org.Lett.|23|6998|doi:10.1021/acs.orglett.1c02706
DOI: 10.5517/ccdc.csd.cc27w6gt
title: CCDC 2081726: Experimental Crystal Structure Determination

description: Related Article: Nikolai Yu. Tiuftiakov, Julia O. Strelnikova, Ilya P. Filippov, Adel R. Khaidarov, Alexander F. Khlebnikov, Alexander S. Bunev, Mikhail S. Novikov, Nikolai V. Rostovskii|2021|Org.Lett.|23|6998|doi:10.1021/acs.orglett.1c02706
DOI: 10.5517/ccdc.csd.cc27wc6q
title: CCDC 2081873: Experimental Crystal Structure Determination

description: Related Article: Qianwen He, Dong Zhang, Fengcai Zhang, Xiaohua Liu, Xiaoming Feng|2021|Org.Lett.|23|6961|doi:10.1021/acs.orglett.1c02588
DOI: 10.5517/ccdc.csd.cc27fb89
title: CCDC 2068390: Experimental Crystal Structure Determination

description: Related Article: Zexiong Pan, Jianying Zhang, Lulu Guo, Hao Yang, Jianfeng Li, Chunming Cui|2021|Inorg.Chem.|60|12696|doi:10.1021/acs.inorgchem.1c01780
DOI: 10.5517/ccdc.csd.cc283xr2
title: CCDC 2089144: Experimental Crystal Structure Determination

description: Related Article: Mario Pacheco, Javier González-Platas, Miguel Julve, Francesc Lloret, Carlos Kremer, Alicia Cuevas|2021|Polyhedron|208|115414|doi:10.1016/j.poly.2021.115414
DOI: 10.5517/ccdc.csd.cc283xs3
title: CCDC 2089145: Experimental Crystal Structure Determination

description: Related Article: Mario Pacheco, Javier González-Platas, Miguel Julve, Francesc Lloret, Carlos Kremer, Alicia Cuevas|2021|Polyhedron|208|115414|doi:10.1016/j.poly.2021.115414
DOI: 10.5517/ccdc.csd.cc284fql
title: CCDC 2089639: Experimental Crystal Structure Determination

description: Related Article: Yuan-Yuan Tang, Jun-Chao Liu, Yu-Ling Zeng, Hang Peng, Xue-Qin Huang, Meng-Juan Yang, Ren-Gen Xiong|2021|J.Am.Chem.Soc.|143|13816|doi:10.1021/jacs.1c06108
DOI: 10.5517/ccdc.csd.cc285xhw
title: CCDC 2091058: Experimental Crystal Structure Determination

description: Related Article: Yao Fu, Xing Liu, Chunhui Shan, Jian Zhou, Wensheng Fu|2021|Inorg.Chem.|60|12724|doi:10.1021/acs.inorgchem.1c02065
DOI: 10.5517/ccdc.csd.cc287kvy
title: CCDC 2092650: Experimental Crystal Structure Determination

description: Related Article: Linhui Zhu, Yiwei Dai, Briana R. Schrage, Christopher J. Ziegler, Li Jia|2021|J.Organomet.Chem.|952|122045|doi:10.1016/j.jorganchem.2021.122045
DOI: 10.5517/ccdc.csd.cc287v8n
title: CCDC 2092911: Experimental Crystal Structure Determination

description: Related Article: Linhui Zhu, Yiwei Dai, Briana R. Schrage, Christopher J. Ziegler, Li Jia|2021|J.Organomet.Chem.|952|122045|doi:10.1016/j.jorganchem.2021.122045
DOI: 10.6084/m9.figshare.16545644.v1
title: 4D volcanic geology of Hachijo-jima islet, Izu-Bonin arc

description: This study combines detailed geologic mapping, tephrochronology, U-Pb zircon dating, and published drill core data to evaluate the time transgressive 3-dimensional (thus 4-dimensional) structure and evolution of the two volcanoes on Hachijo-jima of the juvenile Izu-Bonin intra oceanic arc. The regional Ata-Torihama tephra (Ata-Th; 0.24 Ma) and Kikai-Tozurahara tephra (K-Tz; 0.095 Ma) from Kyushu are intercalated within the voluminous proximal volcanic products. The silicic tuff cover of some supra-subduction zone ophiolites, that formed in a nascent arc setting, may be ancient analogs of the regional tephra derived from the adjacent but mature volcanic arc. The volcanic succession comprises submarine volcanic basement, marine volcaniclastic rocks, and terrestrial tuff intercalated with tholeiitic basalt and the exotic calc-alkaline tephra layers. The undissected Hachijo-Fuji stratovolcano (tholeiitic basalt) overlies marine volcaniclastics abutting the northwestern paleo sea cliffs of the older dissected volcano, Mihara-yama. Newly-described folding and normal faulting of the marine and overlying terrestrial volcaniclastic rocks record NW-SE shortening that may be associated with the collision of the Izu-Bonin arc with the Honshu arc, analogous to the NNE-SSW shortening observed for the Troodos ophiolite. The proto Hachijo-jima volcano emerged above the sea &gt;0.24 Ma, and this date can be applied as a molecular biological calibration date for organisms on this island.
DOI: 10.6084/m9.figshare.16585985
title: Supplementary Material for: The Relationship between the Tissue Expression of TLR2, TLR4, TLR5, and TLR7 and Systemic Inflammatory Responses in Colorectal Cancer Patients

description: <b><i>Background:</i></b> Colorectal cancer (CRC) is the third most commonly diagnosed malignancy globally. CRC patients with elevated plasma C-reactive protein (CRP) levels exhibit compromised prognoses. Toll-like receptors (TLRs), activating the innate and adaptive immune systems, may contribute to pro- and antitumorigenic inflammatory responses. We aimed to identify a possible link between local and systemic inflammatory responses in CRC patients by investigating the association between tissue TLRs and plasma CRP. <b><i>Methods:</i></b> Tissue expressions of TLR2, TLR4, TLR5, and TLR7 were assessed using immunohistochemistry of tissue microarray slides from 549 CRC patients surgically treated between 1998 and 2005. Blood samples were drawn preoperatively, centrifuged, aliquoted, and stored at −80°C until analysis. Plasma CRP was determined through high-sensitivity time-resolved immunofluorometric assay. We investigated the association of TLRs to clinicopathologic variables, plasma CRP, and survival. <b><i>Results:</i></b> High TLR2 expression (hazard ratio [HR] 0.59; 95% confidence interval [CI] 0.41–0.85; <i>p</i> = 0.005), high TLR5 expression (HR 0.60; 95% CI 0.45–0.83; <i>p</i> = 0.002), positive TLR7 expression (HR 0.49; 95% CI 0.33–0.72; <i>p</i> &lt; 0.001), and low CRP (HR 1.48; 95% CI 1.08–2.11; <i>p</i> = 0.017) were associated with a better prognosis. A high TLR2 immunoexpression was associated with a better prognosis among low-CRP patients (HR 0.53; 95% CI 0.35–0.80; <i>p</i> = 0.002), high TLR4 expression among high-CRP patients (HR 2.04; 95% CI 1.04–4.00; <i>p</i> = 0.038), high TLR5 expression among low-CRP patients (HR 0.059; 95% CI 0.37–0.92; <i>p</i> = 0.021), and positive TLR7 expression among low-CRP patients (HR 0.53; 95% CI 0.28–1.00; <i>p</i> = 0.049). In multivariate analyses, no biomarkers emerged as significant independent variables. <b><i>Conclusions:</i></b> High tissue TLR2, TLR5, and TLR7 levels were associated with a better prognosis. Among low-CRP patients, those with high TLR2, TLR5, and TLR7 immunoexpressions exhibited a better prognosis. Among high CRP patients, a high TLR4 immunoexpression was associated with a better prognosis.
DOI: 10.6084/m9.figshare.16585943
title: Supplementary Material for: Combined Orthokeratology with Atropine for Children with Myopia: A Meta-Analysis

description: <b><i>Background:</i></b> Myopia has become a worldwide public health issue, which is occurring at a younger age, leading to an increased risk of high myopia. Ocular complications associated with high myopia can lead to irreversible vision loss. It is urgent and critical to explore effective treatment to slow down or even stop the progression of myopia in young children. <b><i>Objective:</i></b> The aim of the study was to evaluate the additive effects of orthokeratology (OK) and 0.01% atropine ophthalmic solution for myopia in children. <b><i>Methods:</i></b> We searched PubMed, Cochrane Library, EMBASE, MEDLINE, Web of science, Ovid, EBSCO host, CNKI, and CBM to collect eligible studies. Efficacy and safety were evaluated in terms of the axial length (AL), uncorrected distant visual acuity (UCVA), corneal endothelial cell density (CECD), and intraocular pressure (IOP). We calculated the weighted mean difference (WMD) and the 95% confidence intervals (CIs) of all outcomes and plotted on forest plots. <b><i>Results:</i></b> Four studies were ultimately included, involving a total of 267 subjects. This meta-analysis revealed that the mean AL of the subjects in the experimental group was 0.09 mm less than that of subjects in the control group (WMD = −0.09, 95% CI [−0.15, −0.03], <i>p</i> = 0.003). There was no significant difference in UCVA, CECD, and IOP between the 2 groups (WMD was −0.01 [95% CI: −0.03, 0.01], 11.75 [95% CI: −4.09, 27.58], 0.12 [95% CI: −0.40, 0.63], respectively). None of the studies reported severe adverse events. <b><i>Conclusion:</i></b> Our study suggests that the combination of OK and 0.01% atropine is more effective in slowing axial elongation than OK monotherapy in children with myopia in a relatively short duration of treatment. In addition, the combination therapy has no negative influence on UCVA, CECD, and IOP.
DOI: 10.6084/m9.figshare.16585943.v1
title: Supplementary Material for: Combined Orthokeratology with Atropine for Children with Myopia: A Meta-Analysis

description: <b><i>Background:</i></b> Myopia has become a worldwide public health issue, which is occurring at a younger age, leading to an increased risk of high myopia. Ocular complications associated with high myopia can lead to irreversible vision loss. It is urgent and critical to explore effective treatment to slow down or even stop the progression of myopia in young children. <b><i>Objective:</i></b> The aim of the study was to evaluate the additive effects of orthokeratology (OK) and 0.01% atropine ophthalmic solution for myopia in children. <b><i>Methods:</i></b> We searched PubMed, Cochrane Library, EMBASE, MEDLINE, Web of science, Ovid, EBSCO host, CNKI, and CBM to collect eligible studies. Efficacy and safety were evaluated in terms of the axial length (AL), uncorrected distant visual acuity (UCVA), corneal endothelial cell density (CECD), and intraocular pressure (IOP). We calculated the weighted mean difference (WMD) and the 95% confidence intervals (CIs) of all outcomes and plotted on forest plots. <b><i>Results:</i></b> Four studies were ultimately included, involving a total of 267 subjects. This meta-analysis revealed that the mean AL of the subjects in the experimental group was 0.09 mm less than that of subjects in the control group (WMD = −0.09, 95% CI [−0.15, −0.03], <i>p</i> = 0.003). There was no significant difference in UCVA, CECD, and IOP between the 2 groups (WMD was −0.01 [95% CI: −0.03, 0.01], 11.75 [95% CI: −4.09, 27.58], 0.12 [95% CI: −0.40, 0.63], respectively). None of the studies reported severe adverse events. <b><i>Conclusion:</i></b> Our study suggests that the combination of OK and 0.01% atropine is more effective in slowing axial elongation than OK monotherapy in children with myopia in a relatively short duration of treatment. In addition, the combination therapy has no negative influence on UCVA, CECD, and IOP.
DOI: 10.6084/m9.figshare.16585955
title: Supplementary Material for: Ultrasound for Endotracheal Tube Tip Position in Term and Preterm Infants

description: <b><i>Background and Objective:</i></b> Placing an endotracheal tube (ETT) in neonates is challenging and currently requires timely radiographic confirmation of correct tip placement. The objective was to establish the reliability of ultrasound (US) for assessing ETT position in the neonatal intensive care unit (NICU), time needed to do so, and patients’ tolerance. <b><i>Methods:</i></b> A prospective study on 71 newborns admitted to our NICU whose ETT placement was evaluated with US (ETT-echo) and confirmed on chest X-rays (CXR). Data were collected by 3 operators (2 neonatologists and a resident in pediatrics). The right pulmonary artery (RPA) was used as a landmark for US. The distance between the tip of the ETT and the upper margin of the RPA was measured using US and compared with the distance between the tube’s tip and the carina on the CXR. <b><i>Results:</i></b> Seventy-one intubated newborns were included in the study (<i>n</i> = 34 &lt; 1,000 g, <i>n</i> = 18 1,000–2,000 g, <i>n</i> = 19 &gt; 2,000 g). Statistical analysis (Bland-Altman plot and Lin’s concordance correlation coefficient) showed an excellent consistency between ETT positions identified on US and chest X-ray. The 2 measures (ETT-echo and CXR) were extremely concordant both in the whole sample and in the subgroups. Minimal changes in patients’ vital signs were infrequently observed during US, confirming the tolerability of ETT-echo. The mean time to perform US was 3.2 min (range 1–13). <b><i>Conclusions:</i></b> ETT-echo seems to be a rapid, tolerable, and highly reliable method worth further investigating for future routine use in neonatology with a view to reducing radiation exposure.
DOI: 10.6084/m9.figshare.16586015
title: Supplementary Material for: Reference Values for Umbilical Artery Lactate by Mode of Delivery and Gestational Age: A Retrospective Observational Study

description: <b><i>Introduction:</i></b> Umbilical artery cord blood provides information about the intrauterine metabolism during labor. Umbilical artery lactate is a useful parameter in predicting neonatal morbidity, but data on normal values are limited. We aimed to provide reference ranges of umbilical artery lactate values across the combination of gestational age and mode of delivery. <b><i>Methods:</i></b> A single-center retrospective observational study of liveborn infants born between 23 and 42 completed weeks gestation was conducted. Lactate, base deficit (BD), and pH from arterial cord blood were assessed between June 2018 and November 2020 and compared across gestational ages and modes of delivery. <b><i>Results:</i></b> Overall, there were 3,353 infants with evaluable data. Lactate values at the 50th, 90th, and 97th percentile were 3.4, 7.0, and 9.0 mmol/L. Lactate was inversely correlated with pH (<i>r</i> = −0.77, <i>p</i> &lt; 0.001) and positively correlated with BD (<i>r</i> = 0.63, <i>p</i> &lt; 0.001). Lactate values changed significantly across gestational age (Kruskal-Wallis test, <i>p</i> &lt; 0.001) which was attributable to an increase in lactate at 39–41 weeks gestational age. Also, lactate values were different across modes of delivery (Kruskal-Wallis test, <i>p</i> &lt; 0.001) with lowest values in elective CS and highest values in vaginal instrumental deliveries. Comprehensive reference ranges across modes of delivery and gestational ages could be established. <b><i>Discussion:</i></b> Mode of delivery and gestational age contribute to lactate levels with highest values occurring in vaginal instrumental deliveries and between 39 and 41 weeks gestational age. Based on these observations, we provide detailed reference ranges for clinical use.