cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.57760/sciencedb.31808
title: Dnitrification-syn-48h-3.5%
title: 反硝化合成菌群48h3.5%组（塑料生物被膜）

description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 3.5% with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 3.5% with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
DOI: 10.57760/sciencedb.31809
title: Dnitrification-syn-48h-7%
title: 反硝化合成菌群48h7%组（塑料生物被膜）

description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 7%, with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 7%, with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
DOI: 10.57760/sciencedb.31807
title: Dnitrification-syn-24h-7%
title: 反硝化合成菌群24h7%组（塑料生物被膜）

description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 7%, with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 24 h and 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community. 
description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 7%, with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 24 h and 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
DOI: 10.57760/sciencedb.31794
title: Dnitrification-synC3（marine plastic biofilm）
title: 海洋塑料生物被膜反硝化合成菌群对照组3

description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample. 
description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
DOI: 10.57760/sciencedb.31806
title: Dnitrification-syn-24h-3.5%
title: 反硝化合成菌群24h3.5%组（塑料生物被膜）

description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 3.5% with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 24 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
description: To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 3.5% with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 24 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
DOI: 10.57760/sciencedb.31792
title: Dnitrification-synC1（plastic biofilm）
title: 海洋塑料生物被膜反硝化合成菌群对照组1

description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample. 
description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
DOI: 10.57760/sciencedb.31793
title: Dnitrification-synC2（marine plastic biofilm）
title: 海洋塑料生物被膜反硝化合成菌群对照组2

description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample. 
description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
DOI: 10.57760/sciencedb.31795
title: Dnitrification-synT1（marine plastic biofilm）
title: 海洋塑料生物被膜反硝化合成菌群实验组1

description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample. 
description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
DOI: 10.57760/sciencedb.31797
title: Dnitrification-synT3（marine plastic biofilm）
title: 海洋塑料生物被膜反硝化合成菌群实验组3

description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample. 
description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
DOI: 10.57760/sciencedb.31796
title: Dnitrification-synT2（marine plastic biofilm）
title: 海洋塑料生物被膜反硝化合成菌群实验组2

description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample. 
description: On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
DOI: 10.57760/sciencedb.31802
title: Raw data for publication - 10.1039/D5EE02047F
title: Raw data for publication - 10.1039/D5EE02047F

description: Raw data for all plots in the paper: "Surface-Tension Induced High Packing Density Carbon Nanoribbons Film with Ultrahigh Volumetric Capacitance", DOI: https://doi.org/10.1039/D5EE02047F
description: Raw data for all plots in the paper: "Surface-Tension Induced High Packing Density Carbon Nanoribbons Film with Ultrahigh Volumetric Capacitance", DOI: https://doi.org/10.1039/D5EE02047F