cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.w2cps7
title: Occurrence Download

description: A dataset containing 82 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Tridentiger bifasciatus",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 82 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0040094-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/e5066-j8380
title: RodZ oe

description: Raw data files of RodZ oe
description: <strong>Tilt Series Date:</strong> 2009-03-09</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, IMOD</p>
<strong>Collaborators and Roles:</strong> Strains were provided by Anisah Alyahya (Jacobs-Wagner lab). Data collected and processed by Zhuo Li.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> A plasmid pJS14pxylrodZ was electroporated into CB15N. The cells were then grown in PYE plates containing antibiotics. </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-03-09-10/rawdata/</p>09mar09a_bx346_2_010.mrc,  Tilt Series (Pixel Size 0.632 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-03-09-10/rawdata/09mar09a_bx346_2_010.mrc"
        > <i class="download icon"></i> Download </a></p>

        09mar09a_bx346_2_010_part.rec.mrc,  Reconstruction (Pixel Size 1.264 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-03-09-10/3dimage_4476/09mar09a_bx346_2_010_part.rec.mrc"
        > <i class="download icon"></i> Download </a></p>

        zap009.png,  263.3 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-03-09-10/file_2536/zap009.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eqke2-c1x06
title: (D161A)MamK-GFP/MamK_deletion

description: Raw data files of (D161A)MamK-GFP/MamK_deletion
description: <strong>Tilt Series Date:</strong> 2009-11-23</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Magnetospirillum magneticum</p>
<strong>Strain:</strong> AMB-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -16.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> RAPTOR, Peach</p>
<strong>Collaborators and Roles:</strong> Cells provided by Arash Komeili; data collected by ZL.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> (Probably) stationary phase </p>
<strong>Sample Preparation:</strong> Quantifoil R2/2, R2/1, 10nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-10/rawdata/</p>09nov23a_D161AdK_010.mrc,  Tilt Series (Pixel Size 1.192 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-10/rawdata/09nov23a_D161AdK_010.mrc"
        > <i class="download icon"></i> Download </a></p>

        09nov23a_D161AdK_010_part.rec,  Reconstruction (Pixel Size 2.384 nm), 471.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-10/3dimage_9642/09nov23a_D161AdK_010_part.rec"
        > <i class="download icon"></i> Download </a></p>

        09nov23a_D161AdK_010.tif,  1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2009-11-23-10/file_2702/09nov23a_D161AdK_010.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0h5v3-k2962
title: MEFs-Chan Lab

description: MefB cell line from O. Loson in Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-01-05</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-5/3dimage_68795/</p>12jan05_AT1BT1_0005_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-5/3dimage_68795/12jan05_AT1BT1_0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan05_AT1BT1_0005.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-5/rawdata/12jan05_AT1BT1_0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan05_AT1BT1_0005_full_bridge_2membranes.tiff,  file associated to 3D Image #68795, 1.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-5/file_15989/12jan05_AT1BT1_0005_full_bridge_2membranes.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2wkj0-zzv64
title: MEFs-Chan Lab

description: MefB cell line from O. Loson in Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-01-05</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-19/3dimage_68823/</p>12jan05_MEFsB_AT2BT1_0007_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-19/3dimage_68823/12jan05_MEFsB_AT2BT1_0007_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan05_MEFsB_AT2BT1_0007.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-19/rawdata/12jan05_MEFsB_AT2BT1_0007.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan05_MEFsB_AT2BT1_0007_full.tiff,  file associated to 3D Image #68823, 1.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-05-19/file_16003/12jan05_MEFsB_AT2BT1_0007_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dcr57-hz346
title: Bsubtilis_ponA_sacculi

description: Bsubtilis_sacculi_ponA
description: <strong>Tilt Series Date:</strong> 2012-02-11</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Bacillus subtilis </p>
<strong>Strain:</strong> MJ007, ponA null</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain from Kit Pogliano at UCSD</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-1/rawdata/</p>MJsacculi0001.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-1/rawdata/MJsacculi0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        1_full.rec,  Reconstruction (Pixel Size 1.9 nm), 858.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-1/3dimage_25700/1_full.rec"
        > <i class="download icon"></i> Download </a></p>

        MJsacculi0001_full.rec,  Reconstruction (Pixel Size 1.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-02-11-1/3dimage_25701/MJsacculi0001_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/eredd-5er02
title: Agro C58

description: Wild type Agrobacterium C58
description: <strong>Tilt Series Date:</strong> 2013-09-13</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on agar plates for 2 days on Au grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-32/rawdata/</p>C58_au_34k0032.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-32/rawdata/C58_au_34k0032.mrc"
        > <i class="download icon"></i> Download </a></p>

        C58_au_34k0032_part121_63.rec,  Reconstruction (Pixel Size 1.28 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-32/3dimage_30982/C58_au_34k0032_part121_63.rec"
        > <i class="download icon"></i> Download </a></p>

        C58_au_34k0032_part121_63.rec,  Reconstruction (Pixel Size 0.0 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2013-09-13-32/3dimage_49124/C58_au_34k0032_part121_63.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vanzs-5ec85
title: MP TopJ 27.5KX

description: Mycoplasma pneumonia TopJ mutant, bad alignment
description: <strong>Tilt Series Date:</strong> 2010-03-31</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Mycoplasma pneumoniae</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> data collected by Jian; processed by Jian</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-03-31-18/rawdata/</p>10mar31a_B572c_024.mrc,  Tilt Series (Pixel Size 0.774 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-03-31-18/rawdata/10mar31a_B572c_024.mrc"
        > <i class="download icon"></i> Download </a></p>

        10mar31a_B572c_024_part.rec,  Reconstruction (Pixel Size 1.548 nm), 314.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-03-31-18/3dimage_17077/10mar31a_B572c_024_part.rec"
        > <i class="download icon"></i> Download </a></p>

        10mar31a_B572c_024_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-03-31-18/3dimage_55999/10mar31a_B572c_024_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16937079
title: Bo'lajak texnologik ta'lim o'qituvchilarining  grafik kompetentligini transformatsion yondashuv  asosida rivojlantirish texnologiyasini takomillashtirish

description: Ushbu maqolada bo‘lajak texnologik ta’lim o‘qituvchilarining grafik kompetentligini rivojlantirishda transformatsion yondashuvga asoslangan ta’lim texnologiyalarining o‘rni va ahamiyati tahlil qilingan. Ta’lim jarayonida innovatsion yondashuvlar, raqamli vositalar, interaktiv metodlar, loyiha asosida o‘qitish hamda reflektiv tahlil orqali talabalarni grafik ko‘nikmalarga ega bo‘lishga yo‘naltirish usullari yoritilgan. Tadqiqotda konstruktiv, vizual va shaxsga yo‘naltirilgan metodlar uyg‘unligida ishlab chiqilgan transformatsion texnologiya modeli asosida grafik tafakkurni shakllantirish samarali ekani ko‘rsatib berilgan. Shu bilan birga, O‘zbekiston Respublikasining ta’lim sohasidagi islohotlari, ayniqsa PQ-54-sonli Prezident Qarorida belgilangan ustuvor yo‘nalishlar bilan mazkur metodikaning dolzarbligi bog‘lanib, amaliy ahamiyati asoslab berilgan.
DOI: 10.5281/zenodo.16937080
title: Bo'lajak texnologik ta'lim o'qituvchilarining  grafik kompetentligini transformatsion yondashuv  asosida rivojlantirish texnologiyasini takomillashtirish

description: Ushbu maqolada bo‘lajak texnologik ta’lim o‘qituvchilarining grafik kompetentligini rivojlantirishda transformatsion yondashuvga asoslangan ta’lim texnologiyalarining o‘rni va ahamiyati tahlil qilingan. Ta’lim jarayonida innovatsion yondashuvlar, raqamli vositalar, interaktiv metodlar, loyiha asosida o‘qitish hamda reflektiv tahlil orqali talabalarni grafik ko‘nikmalarga ega bo‘lishga yo‘naltirish usullari yoritilgan. Tadqiqotda konstruktiv, vizual va shaxsga yo‘naltirilgan metodlar uyg‘unligida ishlab chiqilgan transformatsion texnologiya modeli asosida grafik tafakkurni shakllantirish samarali ekani ko‘rsatib berilgan. Shu bilan birga, O‘zbekiston Respublikasining ta’lim sohasidagi islohotlari, ayniqsa PQ-54-sonli Prezident Qarorida belgilangan ustuvor yo‘nalishlar bilan mazkur metodikaning dolzarbligi bog‘lanib, amaliy ahamiyati asoslab berilgan.
DOI: 10.22002/27x7h-h9y27
title: VSVGpNL4GFP entry HUVECs

description: HUVECs 30 min post spinoculation with VSVGpNL4GFP
description: <strong>Tilt Series Date:</strong> 2011-08-13</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> HUVECs</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Proviral construct from Irvin Chen UCLA</p>
<strong>Purification / Growth Conditions / Treatment:</strong> infection with VSVG pNL4GFP by spinoculation</p>
<strong>Sample Preparation:</strong> manual blot</p>
  11aug13_BATCH_0031.jpg: file associated to 3D Image #54296
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-13-20/rawdata/</p>11aug13_BATCH_0031.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-13-20/rawdata/11aug13_BATCH_0031.mrc"
        > <i class="download icon"></i> Download </a></p>

        11aug13_BATCH_0031_full.rec,  Reconstruction (Pixel Size 2.0 nm), 516.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-13-20/3dimage_54296/11aug13_BATCH_0031_full.rec"
        > <i class="download icon"></i> Download </a></p>

        11aug13_BATCH_0031.tiff,  file associated to 3D Image #54296, 1.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2011-08-13-20/file_13057/11aug13_BATCH_0031.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gy6kz-pq915
title: MP wt 27.5KX

description: Mycoplasma pneumonia wt mutant, empty
description: <strong>Tilt Series Date:</strong> 2010-02-21</p>
<strong>Data Taken By:</strong> Jian Shi</p>
<strong>Species / Specimen:</strong> Mycoplasma pneumoniae</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> data collected by Jian; processed by Jian</p>
<strong>Sample Preparation:</strong> QF grid, 10nm gold, 100% humidity...</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-02-21-9/rawdata/</p>10feb21a_MP3a_009.mrc,  Tilt Series (Pixel Size 0.774 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-02-21-9/rawdata/10feb21a_MP3a_009.mrc"
        > <i class="download icon"></i> Download </a></p>

        10feb21a_MP3a_009_full.rec,  Reconstruction (Pixel Size 1.548 nm), 419.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-02-21-9/3dimage_17125/10feb21a_MP3a_009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        10feb21a_MP3a_009_SIRT.rec,  Reconstruction (Pixel Size 0.0 nm), 524.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/jsa2010-02-21-9/3dimage_55971/10feb21a_MP3a_009_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.29975296
title: Comprehensive Chemical Profiling and In Vivo Metabolism of ASDZL Formula Using UHPLC-HRMS and Molecular Networking

description: In this metabolomics study, the chemical composition of AnShenDingZhiLing(ASDZL), a traditional Chinese medicine formula composed of twelve herbal medicines, was characterized by ultra-performance liquid chromatography combined with high-resolution mass spectrometry (UHPLC-HRMS) system, and its absorption prototypes and metabolites were identified in rat plasma and brain.Raw data was acquired in both positive and negative ionization modes using information-dependent acquisition (IDA) on the AB SCIEX ZenoTOF 7600 System. Data processing is performed using MZmine 4.3.0 for feature detection and alignment. The processed data is then analyzed using a feature-based molecular network (FBMN) on the GNPS platform to aid in compound annotation based on spectral similarity.A total of 243 compounds were identified or preliminarily characterized in the ASDZL formulation, including flavonoids, terpenoids, phenylpropionyl glycosides, alkaloids, anthraquinones, phenylethanol glycosides, and other chemical classes. In addition, <i>in vivo</i> . Key metabolic reactions observed include glucuronidation, sulfation, methylation, and hydroxylation. This dataset provides a comprehensive chemical basis for understanding the pharmacologically active components of ASDZL and provides a valuable resource for further research on its mechanism of action and quality control. Raw and processed mass spectrometry data is stored in this repository for community reuse and further exploration.
DOI: 10.6084/m9.figshare.29975296.v1
title: Comprehensive Chemical Profiling and In Vivo Metabolism of ASDZL Formula Using UHPLC-HRMS and Molecular Networking

description: In this metabolomics study, the chemical composition of AnShenDingZhiLing(ASDZL), a traditional Chinese medicine formula composed of twelve herbal medicines, was characterized by ultra-performance liquid chromatography combined with high-resolution mass spectrometry (UHPLC-HRMS) system, and its absorption prototypes and metabolites were identified in rat plasma and brain.Raw data was acquired in both positive and negative ionization modes using information-dependent acquisition (IDA) on the AB SCIEX ZenoTOF 7600 System. Data processing is performed using MZmine 4.3.0 for feature detection and alignment. The processed data is then analyzed using a feature-based molecular network (FBMN) on the GNPS platform to aid in compound annotation based on spectral similarity.A total of 243 compounds were identified or preliminarily characterized in the ASDZL formulation, including flavonoids, terpenoids, phenylpropionyl glycosides, alkaloids, anthraquinones, phenylethanol glycosides, and other chemical classes. In addition, <i>in vivo</i> . Key metabolic reactions observed include glucuronidation, sulfation, methylation, and hydroxylation. This dataset provides a comprehensive chemical basis for understanding the pharmacologically active components of ASDZL and provides a valuable resource for further research on its mechanism of action and quality control. Raw and processed mass spectrometry data is stored in this repository for community reuse and further exploration.