cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.14457/tu.the.2014.661
title: China: the big exporter ; its dark side ; the case of fake food

description: China has long been considered as a turf for counterfeiting industry. Fake goods that are produced in China are sold throughout the entire world because the prices of the items purchased are incredibly low. That dominance has piloted China into a similar (product) based industry. An industry I shall term the fake food industry that undermining health and induce sickness to the consumers. The food market itself reaps a higher than normal marketability due to overpopulation and world food scarcity. The price of food goes up but still people demand for cheaper food. Melamine has been disguised into “milk powder”, “soy protein concentrate” and “gluten” (wheat flour) as a form of cheap ingredient for China’s food industry. It can also easily relocate or infiltrate in to other food industries in many parts of the world. Melamine alone is of low toxicity, but however when melamine is combined with cyanuric acid leads to crystal formation and subsequent kidney toxicity.Instances of counterfeit merchandise distribution and fake food distribution have been identified as following a similar system of movement and have been recognized as having the same country of origin. The big difference is consumers who buy counterfeit goods are mostly aware that these products are faked due to the fact that they demonstrate themselves to be of lesser quality to their legitimate product and price comparatives. In the case of fake food, the distinction is more effectively hidden and unclear to the public at large. Consumers are simply unaware of the hazards associated with these products. This study also unearths exactly why an individual country like China cannot ensure the food safety standard and content quality of its food products.
DOI: 10.14457/tu.the.2018.442
title: ปัจจัยการยอมรับโมบายแอพพลิเคชั่นสำหรับส่งสินค้า: กรณีศึกษา ลาล่ามูฟ (Lalamove)

description: การศึกษาวิจัยนี้เป็นการวิจัยเชิงปริมาณ (Quantitative Research) มีวัตถุประสงค์ คือ (1) เพื่อศึกษาปัจจัยการยอมรับโมบายแอพพลิเคชั่นสำหรับส่งสินค้า กรณีศึกษา ลาล่ามูฟ (Lalamove) (2) เพื่อทดสอบปัจจัยต่างๆ ที่เกี่ยวข้องกับการยอมรับการใช้งานโมบายแอพพลิเคชั่น ลาล่ามูฟ (Lalamove) โดยใช้แบบสอบถามที่ผ่านการทดสอบความเชื่อมั่นเป็นเครื่องมือในการเก็บรวบรวมข้อมูล กลุ่มตัวอย่างที่ใช้ในการศึกษาวิจัย คือ ผู้ใช้งานแอพพลิเคชั่นลาล่ามูฟ ในกรุงเทพมหานครและปริมณฑล (ซึ่งเป็นเขตบริการของโมบายแอพพลิเคชั่นลาล่ามูฟ) จำนวน 400 คน และดำเนินการวิเคราะห์ข้อมูลด้วยสถิติเชิงพรรณนา (Descriptive Statistic) และสถิติเชิงอนุมาน (Inferential Statistic) โดยใช้การวิเคราะห์ , องค์ประกอบเชิงยืนยัน (Confirmatory Factor Analysis: CFA) , การวิเคราะห์โมเดลงานวิจัยด้วยวิธีสมการโครงสร้าง (Structural Equation Modeling: SEM)จากผลการวิจัย พบว่าปัจจัยที่ส่งผลต่อการยอมรับโมบายแอพพลิเคชั่น จากผู้ใช้งานแอพพลิเคชั่นลาล่ามูฟ ในกรุงเทพมหานครและปริมณฑล โดยมีทั้งหมด 12 ปัจจัย พบว่าผลจากการศึกษา ปัจจัยที่ส่งผลต่อการยอมรับการใช้งานแอพลิเคชั่นลาล่ามูฟ คือ การรับรู้ถึงประโยชน์ (Perceived Usefulness) ในด้านปัจจัยความเหมาะสมของข้อมูล (Information Fit to Task) และ ด้านความปลอดภัยของแอพพลิเคชั่น (Security)
DOI: 10.14457/tu.the.2018.443
title: ปัจจัยทางด้านความสัมพันธ์ต่อพฤติกรรมของผู้บริโภคที่มีผลในการตัดสินใจเข้าเยี่ยมชมและซื้อสินค้าผ่านช่องทาง social commerce: บทบาท ลักษณะเชิงชั่วคราวและลักษณะเชิงถาวร

description: การวิจัยครั้งนี้มีวัตถุประสงค์เพื่อศึกษา ปัจจัยลักษณะเชิงชั่วคราว ลักษณะเชิงถาวรความไว้วางใจ และลักษณะของโซเชียลคอมเมิร์ซ ที่มีความสัมพันธ์ต่อการเข้าเยี่ยมชมและความตั้งใจซื้อผ่านโซเชียลคอมเมิร์ซ กลุ่มตัวอย่าง คือ ผู้บริโภคที่เคยซื้อสินค้าออนไลน์อายุระหว่าง 18 –34 ปีจานวน 532 คน ศึกษาโดยใช้แบบสอบถามเป็นเครื่องมือในการเก็บรวบรวมข้อมูล ทาการวิเคราะห์ข้อมูลการวิเคราะห์โมเดลด้วยสมการโครงสร้างผลการวิจัยพบว่า ลักษณะเชิงชั่วคราว ลักษณะเชิงถาวร และความไว้วางใจมีความสัมพันธ์เชิงบวกต่อความตั้งใจเข้าเยี่ยมชม และความตั้งใจเข้าเยี่ยมชมมีความสัมพันธ์เชิงบวกต่อความตั้งใจซื้อ ผลการวิจัยครั้งนี้ช่วยให้ผู้ประกอบธุรกิจโซเชียลคอมเมิร์ซพัฒนารูปแบบธุรกิจและกลยุทธ์ที่มีประสิทธิภาพ
DOI: 10.14457/tu.the.2018.444
title: ปัจจัยที่มีอิทธิพลต่อการตัดสินใจซื้อสินค้าแบรนด์เนมประเภทไฮเอนด์ผ่านช่องทางพาณิชย์อิเล็กทรอนิกส์ (e-commerce) ในบริบทของ Facebook

description: การค้นคว้าอิสระนี้มีวัตถุประสงค์เพื่อศึกษาปัจจัยที่มีอิทธิพลต่อการตัดสินใจซื้อสินค้า แบรนด์เนมประเภทไฮเอนด์ผ่านช่องทางพาณิชย์อิเล็กทรอนิกส์ (E-commerce) ในบริบทของ เฟซบุค (Facebook) ผลของการศึกษาจะช่วยให้ผู้ประกอบการที่ทําธุรกิจการขายสินค้าผ่านทาง เฟซบุค (Facebook) สามารถนําข้อมูลที่ได้ไปใช้วางแผนปรับปรุงหรือพัฒนาธุรกิจต่อไปได้ งานวิจัยนี้ เป็น งานวิจัยเชิงปริมาณ กลุ่มตัวอย่างคือ ผู้ที่เคยซื้อสินค้าแบรนด์เนมประเภทไฮเอนด์ผ่านช่องทาง พาณิชย์อิเล็กทรอนิกส์ (E-commerce) ในบริบทของเฟซบุค (Facebook) จํานวน 400 คน โดยใช้ แบบสอบถามออนไลน์เป็นเครื่องมือในการเก็บรวบรวมข้อมูล สถิติที่ใชใ้ นการวิเคราะห์ข้อมูล คือ สถิติ เชิงพรรณา ประกอบด้วย ความถี่ ร้อยละ ค่าเฉลี่ยและส่วนเบี่ยงเบนมาตรฐาน และสถิติเชิงปริมาณ ประกอบด้วย Independent Sample t-test, One-way ANOVA,และRegressionผลการศึกษาพบว่า ปัจจัยที่มีอิทธิพลต่อการตัดสินใจซื้อสินค้าแบรนด์เนมประเภทไฮเอนด์ ผ่านช่องทางพาณิชย์อิเล็กทรอนิกส์ (E-commerce) ในบริบทของเฟซบุค (Facebook) มีเพียง 3 ปัจจัยที่ส่งผลคือด้านความน่าเชื่อถือ ด้านอิทธิพลทางสังคมและด้านการรับรู้ประโยชน์ ในขณะที่ ปัจจัยทางด้านทัศนคติ ด้านราคา ด้านส่งเสริมการตลาด ด้านความเสี่ยงทางการเงินและด้านความ เสี่ยงทางสินค้าไม่ส่งผลต่อการตัดสินใจซื้อสินค้าแบรนด์เนมประเภทไฮเอนด์ผ่านช่องทางพาณิชย์ อิเล็กทรอนิกส์ (E-commerce) ในบริบทของเฟซบุค (Facebook)
DOI: 10.18739/a2f47gt75
title: Beaufort Gyre Observing System (BGOS) Mooring in situ carbon dioxide partial pressure (pCO2) and pH time-series, Beaufort Sea, Arctic Ocean, 2017-2018
DOI: 10.5281/zenodo.3243966
title: DMS emission dataset (latitudes &gt;45N)

description: # README file for (sub)Arctic DMS emission dataset (latitudes &gt;45N), v1.0.0<br> =========================================================================== Martí Galí Tàpias, 2019-06-12<br> http://orcid.org/0000-0002-5587-1271 Address requests for additional data and questions to:<br> marti.gali.tapias@gmail.com This dataset contains satellite-derived time series of:<br> * dmspt (sea-surface dimethylsulfoniopropionate -DMSPt- concentration, in µmol/m3 or equivalent nmol/L)<br> * dms (sea-surface dimethylsulfide -DMS- concentration, in µmol/m3 or equivalent nmol/L)<br> * fdms (sea-air DMS flux, in µmol/m2/d)<br> * ice concentration (0 to 1) The DMSPt dataset was produced as described by:<br> Galí, M., Devred, E., Levasseur, M., Royer, S. J., & Babin, M. (2015). A remote sensing algorithm for planktonic dimethylsulfoniopropionate (DMSP) and an analysis of global patterns. Remote Sensing of Environment, 171, 171-184. https://doi.org/10.1016/j.rse.2015.10.012 The DMS dataset was produced as described by: Galí, M., Devred, E., Babin, M., & Levasseur, M. (2019). Decadal increase in Arctic dimethylsulfide emission. Accepted in PNAS. doi:10.1073/pnas.1904378116. Galí, M., Levasseur, M., Devred, E., Simó, R., & Babin, M. (2018). Sea-surface dimethylsulfide (DMS) concentration from satellite data at global and regional scales. Biogeosciences, 15(11), 3497-3519. https://doi.org/10.5194/bg-15-3497-2018 Code for the DMSPt_SAT and DMS_SAT algorithms: https://github.com/mgali/DMS-SAT_ALGORITHM <br> ## File format MetCDF files are self-describing: they contain one “variable of interest” and all the corresponding temporal and geographic dimensions. ## File naming convention variable_sensorYYYY_nD_28km.nc Both the DMSPt and DMS satellite datasets are based on ocean color data obtained from NASA Ocean Color website. Chlorophyll was derived from the GSM algorithm, hence _gsm was appended to the variable names. DMSPt and DMS were produced for 2 satellite sensors.<br> * S = SeaWiFS, years 1998 to 2007<br> * A = MODIS-Aqua, years 2003 to 2016 (the last year is empty from October on) FDMS was calculated using ERA-Interim wind speed and SST, and ice cover from NSIDC. Two different gas exchange parameterizations were used, whose names are appended to the variable names:<br> * W97 (Woolf 1997)<br> * N00GBC (Nightingale et al. 2000 GBC –do not confound with Nightingale et al. 2000 GL) FDMS was also calculated using climatological DMS fields (W97 parameterization) while retaining the interannual variability of all the other variables involved in FDMS calculation:<br> * S19982007 DMS climatology<br> * A20032016 DMS climatology<br> * L11 DMS climatology (interpolation based climatology of Lana et al. 2011 GBC) Ice cover (ice) is also provided for the full period 1998-2016. ## Directory naming convention Each directory is named using the names of variable it contains.
DOI: 10.5281/zenodo.3243967
title: DMS emission dataset (latitudes &gt;45N)

description: # README file for (sub)Arctic DMS emission dataset (latitudes &gt;45N), v1.0.0<br> =========================================================================== Martí Galí Tàpias, 2019-06-12<br> http://orcid.org/0000-0002-5587-1271 Address requests for additional data and questions to:<br> marti.gali.tapias@gmail.com This dataset contains satellite-derived time series of:<br> * dmspt (sea-surface dimethylsulfoniopropionate -DMSPt- concentration, in µmol/m3 or equivalent nmol/L)<br> * dms (sea-surface dimethylsulfide -DMS- concentration, in µmol/m3 or equivalent nmol/L)<br> * fdms (sea-air DMS flux, in µmol/m2/d)<br> * ice concentration (0 to 1) The DMSPt dataset was produced as described by:<br> Galí, M., Devred, E., Levasseur, M., Royer, S. J., & Babin, M. (2015). A remote sensing algorithm for planktonic dimethylsulfoniopropionate (DMSP) and an analysis of global patterns. Remote Sensing of Environment, 171, 171-184. https://doi.org/10.1016/j.rse.2015.10.012 The DMS dataset was produced as described by: Galí, M., Devred, E., Babin, M., & Levasseur, M. (2019). Decadal increase in Arctic dimethylsulfide emission. Accepted in PNAS. doi:10.1073/pnas.1904378116. Galí, M., Levasseur, M., Devred, E., Simó, R., & Babin, M. (2018). Sea-surface dimethylsulfide (DMS) concentration from satellite data at global and regional scales. Biogeosciences, 15(11), 3497-3519. https://doi.org/10.5194/bg-15-3497-2018 Code for the DMSPt_SAT and DMS_SAT algorithms: https://github.com/mgali/DMS-SAT_ALGORITHM <br> ## File format MetCDF files are self-describing: they contain one “variable of interest” and all the corresponding temporal and geographic dimensions. ## File naming convention variable_sensorYYYY_nD_28km.nc Both the DMSPt and DMS satellite datasets are based on ocean color data obtained from NASA Ocean Color website. Chlorophyll was derived from the GSM algorithm, hence _gsm was appended to the variable names. DMSPt and DMS were produced for 2 satellite sensors.<br> * S = SeaWiFS, years 1998 to 2007<br> * A = MODIS-Aqua, years 2003 to 2016 (the last year is empty from October on) FDMS was calculated using ERA-Interim wind speed and SST, and ice cover from NSIDC. Two different gas exchange parameterizations were used, whose names are appended to the variable names:<br> * W97 (Woolf 1997)<br> * N00GBC (Nightingale et al. 2000 GBC –do not confound with Nightingale et al. 2000 GL) FDMS was also calculated using climatological DMS fields (W97 parameterization) while retaining the interannual variability of all the other variables involved in FDMS calculation:<br> * S19982007 DMS climatology<br> * A20032016 DMS climatology<br> * L11 DMS climatology (interpolation based climatology of Lana et al. 2011 GBC) Ice cover (ice) is also provided for the full period 1998-2016. ## Directory naming convention Each directory is named using the names of variable it contains.
DOI: 10.14457/cu.the.2011.26
title: Effect of vitamin E supplementation on inflammatory markers in patients with Thalassemia intermedia at Siriraj hospital

description: The purpose of this study was to investigate the effects of vitamin E supplementation on inflammatory markers in patients with thalassemia intermedia who do not required regular blood transfusion at Siriraj hospital. There were 31 patients aged 5 - 19 years participating in this study. They were divided into 2 groups: the vitamin E group (supplemented with vitamin E at dose of 10 IU/kg/d, n = 16) and the control group (no vitamin E supplementation, n = 15). The duration of the study was 12 weeks. The patients received growth, nutritional and dietary intake assessments. In addition, their blood samples were collected for determining levels of plasma inflammatory markers (tumor necrosis factor-α (TNF-α) and high sensitivity C-reactive protein (hs-CRP)), serum vitamin E, and complete blood count (CBC) at baseline and at the end of the study (week 12). The results of the study showed that at the baseline, no correlations between level of serum vitamin E and inflammatory markers including TNF-α and hs-CRP were found. However, there was a positive correlation between serum vitamin E levels and red blood cell (r = 0.503, p = 0.002). After the patients were assigned into the vitamin E and the control groups, there were no significant differences in demographic characteristics, biological and hematological variables including plasma TNF-α and hs-CRP levels, serum vitamin E levels, and CBC between the patients in both groups. After 12 weeks of the study, serum vitamin E concentrations in the patients supplemented with vitamin E were significantly increased from their baselines (p &lt; 0.001). However, plasma TNF-α and hs-CRP levels of the patients in both groups did not change from the baseline after vitamin E supplementation. It was found that total energy intake of the patients in the vitamin E group at week 12 were significantly increased from baseline (p = 0.037) and that amount was significantly greater than that in the control group (p = 0.008). Moreover, it was found that after vitamin E supplementation, weight and body mass index of some patients were improved to the better categories. This study revealed that vitamin E supplementation did not affect the levels of TNF-α and hs-CRP in patients with thalassemia intermedia. However, it may be beneficial in ameliorating vitamin E levels of these patients to the normal range and increasing total energy intake from the diet.
DOI: 10.14457/cu.the.2011.27
title: Propyloleate production using immobilized lipase

description: Biolubricants derived from vegetable oils and fatty acids are environmentally compatible products due to their low toxicity and good biodegradability. In this work, biolubricant production by an esterification reaction of oleic acid and propanol was studied, where immobilized Novozym 435 lipase enzyme was used as a biocatalyst. The experiments were carried out in a shaking incubator to investigate effect of reaction time, alcohol structure (propanol vs. isopropanol), enzyme loading, rotation speed, molar ratio of propanol to oleic acid, and reaction temperature. The optimal condition were 45oC, molar ratio of PrOH to Oleic acid of 2:1, Novozym 435 loading at 5% based on oleic acid weight and 250 rpm, in which the maximal FFA conversion at 88.9% was obtained. It was shown that the FFA conversion could be increased to 94.7% (or 6.5% increases) by removal of water during the reaction using molecular sieve. From kinetic study, the activation energy of the esterification reaction was 34.05 kJ/mol. Finally, it demonstrated that Novozym 435 could be used at least 5 cycles without considerable change in the conversion for propyloleate production.
DOI: 10.14457/cu.the.2011.28
title: Marital satisfaction and cultural integration of cross-cultural couples : a case study of Indonesian women married to Korean men

description: This research aimed to study the relationship between level of marital satisfaction and cultural integration of Indonesian women who married to Korean men, who are at the time of research living in South Korea. Marital satisfaction is examined based on couples’ conjugal life and three aspects (Korean language fluency, socialization, and living adjustment) are used to examine cultural integration. Result indicated that, demographically, respondents were varied in their province of origin in Indonesia. Most of them were in their thirties, high school degree holder, and mothers of primary school aged children. Two third of respondents were not merely housewives, none of them met their husband through marriage broker like other marriage migrant from Southeast Asia countries. In fact, most of respondents met their Korean husband in Indonesia through individual meeting. Marital satisfaction of respondents based on their conjugal life is considered high as well as their integration into the society in terms of the ability of speaking Korean and socialization. Overall, the level of integration is up to medium level. vThe examination leads to the result that most respondents are satisfied with their marriage and also well-integrate into Korean society. However, level of satisfaction on marriage life is not a direct factor to determine the level of integration. The most important factor actually come from respondents themselves, that is their eager will to integrate into Korean society. This explained why in the study we found respondents with high level satisfaction on her marriage but could not speak Korean well, less interact with Korean people and respondents who are very unsatisfied with her marriage but speak Korean very good, have many Korean friends. The eager will is supported by respondents’ independence. They have freedom to do many things besides housework and could utilize that freedom well. This freedom is likely provided by their Korean husbands who do not restrict their foreign wives from self-actualization.
DOI: 10.14457/cu.the.2011.29
title: Autologous bone marrow aspirate (BMA) derived stem cells for chronic wounds of the lower extremity

description: The use of stem cells in chronic and problematic wounds is of particular interest as chronic wounds, particularly those of a diabetic nature, are a physical, social and financial drain to both the patient and the medical system. Expediting wound closure and reducing risk of amputation then becomes important. Stem cells in chronic wounds may assist with cell migration, proliferation and the repair process. In this article, we have retrospectively reviewed patients that were treated with autologous bone marrow aspirate (BMA) in an attempt to determine any potential value in expediting wound closure. A comprehensive review of the literature on the treatment of chronic wounds through the use of autologous mesenchymal stem cells was performed and have examined as a novel clinical technique in the treatment of chronic wound
DOI: 10.14457/cu.the.2011.30
title: Effects of curcumin on diabetes-induced leukocyte-endothelium interaction: roles of nox2 enzyme and thioredoxin-interacting protein

description: Hyperglycemia increases thioredoxin-interacting protein (Txnip) expression that causes cellular redox imbalance. In addition, the up-regulation of Txnip can enhance vascular cell adhesion molecules expression such as intercellular adhesion molecule-1 (ICAM-1) which leads to increase leukocyte-endothelium interaction to promote vascular inflammation. Moreover, the leukocyte accumulation in microcirculation causes endothelial damage by NADPH oxidase enzyme (NOX2). Curcumin is a polyphenolic compound which exhibits antioxidant, and anti-inflammatory properties. The present study aimed to study the mechanisms of curcumin on preventing diabetes-induced leukocyte-endothelium interaction in association with its actions on NOX2 enzyme and Txnip expressions. Male Wistar rats were divided into four groups: control-treated with corn oil group (CON), control-treated with curcumin group (CONCUR, 300mg/kgBW), diabetic-treated with corn oil group (DM; streptozotocin (STZ), i.v. 55 mg/kg BW), and diabetes-treated with curcumin (DMCUR). The supplementation of curcumin was started at 10 days after STZ-injection. Then on the 12th week after STZ injection, iris blood perfusion and leukocyte adhesion at iris of each rat was measured by using laser Doppler and intravital fluorescent microscopy, respectively. Plasma glucose and HbA1c were also determined by using enzymatic and turbidimetric immunoinhibition methods, respectively. P47phox expression (marker of NOX2 enzyme activation), Txnip expression, and malondialdehyde (MDA) level at eye fundus were determined by Western Blot analysis and TBAR assay, respectively. The results showed that body weight and iris blood perfusion of DM and DMCUR were decreased significantly as compared to CON (P &lt; 0.001), but plasma glucose and HbA1c of DM and DMCUR were increased significantly as compared to CON (P &lt; 0.001). Interestingly, the leukocyte adhesion, p47phox expression, and MDA level in DM were increased significantly as compared to CON (P &lt; 0.05), but decreased significantly when compared to DMCUR (P &lt; 0.05). It is concluded that 300 mg/kgBW curcumin could ameliorate diabetic vascular inflammation by decreasing ROS overproduction, reducing leukocyte-endothelium interaction, and also inhibiting NOX2 activation. However, it could not significantly reduce blood glucose, Txnip expression, and increase iris blood perfusion.
DOI: 10.14457/cu.the.2011.31
title: Strategies and improve sensitivity of nucleic acid lateral flow (NALF) in HIV-1 nucleic acid

description: Nucleic Acid Lateral Flow (NALF) is a rapid test for nucleic acid detection that is easy to perform and to interpret. This advantage is suitable for a pathogen diagnosis in low resource settings. However, the major weakness is it has limited sensitivity. Thus, this study aimed to setup and improve sensitivity of NALF to detect HIV-1 nucleic acid. The strategies including UV crosslink, enzyme link gold nanoparticles conjugate and Helicase Dependent Isothermal Amplification (HDA). The NALF platform for HIV-1 detection were setup and optimized. UV exposure was performed to increase the capture probe adherence to the membrane. The results revealed that the streptavidin/biotin combined with UV crosslink method had increased approximately 30% of signal level when compare to streptavidin/biotin alone. Whereas, Non-labeled oligonucleotides combined with UV crosslinking gave a comparable result to the streptavidin/biotin method. HRP linked-gold nanoparticle approach increased approximately 3 folds of signal level or 300% (p&lt;0.05) when compared to the conventional method. HDA amplification using HIV-1 cDNA from clinical samples with known viral load then deteced by the NALF system was able detect at least 445 copies/mL of HIV-1 RNA from plasma (pre-amplification template).
DOI: 10.14457/cu.the.2011.32
title: Fabrication of gold Nanoparticles/pyrrole/hrp electrode for phenol biosensor by electropolymerization

description: This research is the investigation of the modified screen-printed carbon electrode with gold nanoparticles (AuNPs) and polypyrrole (PPy) together with the immobilized horseradish peroxidase by electropolymerization approach for phenol detection. The optimized electropolymerization conditions were 5 mM of gold precursor concentration and 10 cycling numbers of electropolymerization, respectively. The results of UV-Vis spectroscopy indicated the non-denaurization of HRP when mixed in solutions containing pyrrole and gold precursor which were of spherical shape and an average diameter approximately 15±5 nm. After that, the synthesized modified electrodes were physically characterized by SEM and AFM. AuNPs/PPy film revealed small granular particles mixed withrod-like structure. Whereas EDX results confirmed the presence and uniform distribution of AuNPs on the effective electrode surface area. Futhermore, the direct electron transfer was a typical of surface-controlled quasi-reversible process with the electron transfer rate of 0.05s-1. The study of enzyme kinetic parameters, we found that the mechanism did not follow Michaelis-Menten kinetics. The resulting biosensor performed a linear range from 1-8 µM, with sensitivity of 4.585nA/µM (R2= 0.955), detection limit of 3.19 µM (n=10), (S/N=3), and fast response time of 23.5 s. this biosensor showed a storage stability of residual current was 83.37% after seven days of storage in dried form at 4°C, and reproducibility was 3.78% (n=10).