cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7941989
title: Intramyocardial implantation of differentiated rat bone marrow mesenchymal stem cells enhanced by TGF-β1 improves cardiac function in heart failure rats

description: The present study tested the hypotheses that i) transforming growth factor beta 1 (TGF-β1) enhances differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards the cardiomyogenic phenotype and ii) intramyocardial implantation of the TGF-β1-treated MSCs improves cardiac function in heart failure rats. MSCs were treated with different concentrations of TGF-β1 for 72 h, and then morphological characteristics, surface antigens and mRNA expression of several transcription factors were assessed. Intramyocardial implantation of these TGF-β1-treated MSCs to infarcted heart was also investigated. MSCs were initially spindle-shaped with irregular processes. On day 28 after TGF-β1 treatment, MSCs showed fusiform shape, orientating parallel with one another, and were connected with adjoining cells forming myotube-like structures. Immunofluorescence revealed the expression of cardiomyocyte-specific proteins, α-sarcomeric actin and troponin T, in these cells. The mRNA expression of GATA4 and Nkx2.5 genes was slightly increased on day 7, enhanced on day 14 and decreased on day 28 while α-MHC gene was not expressed on day 7, but expressed slightly on day 14 and enhanced on day 28. Transmission electron microscopy showed that the induced cells had myofilaments, z line-like substances, desmosomes, and gap junctions, in contrast with control cells. Furthermore, intramyocardial implantation of TGF-β1-treated MSCs to infarcted heart reduced scar area and increased the number of muscle cells. This structure regeneration was concomitant with the improvement of cardiac function, evidenced by decreased left ventricular end-diastolic pressure, increased left ventricular systolic pressure and increased maximal positive pressure development rate. Taken together, these results indicate that intramyocardial implantation of differentiated MSCs enhanced by TGF-β1 improved cardiac function in heart failure rats.
DOI: 10.6084/m9.figshare.7941989.v1
title: Intramyocardial implantation of differentiated rat bone marrow mesenchymal stem cells enhanced by TGF-β1 improves cardiac function in heart failure rats

description: The present study tested the hypotheses that i) transforming growth factor beta 1 (TGF-β1) enhances differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards the cardiomyogenic phenotype and ii) intramyocardial implantation of the TGF-β1-treated MSCs improves cardiac function in heart failure rats. MSCs were treated with different concentrations of TGF-β1 for 72 h, and then morphological characteristics, surface antigens and mRNA expression of several transcription factors were assessed. Intramyocardial implantation of these TGF-β1-treated MSCs to infarcted heart was also investigated. MSCs were initially spindle-shaped with irregular processes. On day 28 after TGF-β1 treatment, MSCs showed fusiform shape, orientating parallel with one another, and were connected with adjoining cells forming myotube-like structures. Immunofluorescence revealed the expression of cardiomyocyte-specific proteins, α-sarcomeric actin and troponin T, in these cells. The mRNA expression of GATA4 and Nkx2.5 genes was slightly increased on day 7, enhanced on day 14 and decreased on day 28 while α-MHC gene was not expressed on day 7, but expressed slightly on day 14 and enhanced on day 28. Transmission electron microscopy showed that the induced cells had myofilaments, z line-like substances, desmosomes, and gap junctions, in contrast with control cells. Furthermore, intramyocardial implantation of TGF-β1-treated MSCs to infarcted heart reduced scar area and increased the number of muscle cells. This structure regeneration was concomitant with the improvement of cardiac function, evidenced by decreased left ventricular end-diastolic pressure, increased left ventricular systolic pressure and increased maximal positive pressure development rate. Taken together, these results indicate that intramyocardial implantation of differentiated MSCs enhanced by TGF-β1 improved cardiac function in heart failure rats.
DOI: 10.6084/m9.figshare.7941974
title: Alternative treatments for the conservation of castor seeds (Ricinus communis L.)

description: ABSTRACT The aim of this study was to evaluate the effects of alternative treatments and storage environment on the physiological and sanitary qualities of castor seeds. Treatments were: derosal, vitavax-thiram, sodium benzoate, potassium sorbate, trichodermil SP, rosemary, garlic, cinnamon, Indian clove and control treatment. The seeds were subjected to storage in laboratory and in a cold chamber for 12 months. Castor seeds chemically treated with derosal and vitavax-thiram showed physiological and sanitary quality superior to those of seeds treated with alternative products and without treatment at 12 months of storage. Castor seeds stored in laboratory, for a period longer than 8 months, must be treated with traditional chemical fungicides. If the seeds are stored in a cold chamber, the maintenance of their germination and vigor above 80% does not depend on the used fungicide treatment. The most promising alternative fungicides were trichodermil, potassium sorbate and garlic, especially the first product.
DOI: 10.6084/m9.figshare.7941980
title: Physiological analysis in coffee seedlings showing brown eye spot under different irrigation levels.

description: ABSTRACT Abstract: Brown eye spot is one of the major diseases that affect coffee seedling growth for causing defoliation and reducing the plant growth. The disease severity is higher when the coffee plant is subjected to water deficit. Using irrigation is an alternative to minimize the damage caused by this disease. The aim of this study was to evaluate the impact of this disease on the physiology of coffee plants as a function of the reduction in the irrigation level. The plants were subjected to three irrigation levels (0.1, 0.6 and 1.2 mm.day-1), inoculated and not inoculated with Cercospora coffeicola, in a completely randomized design with 35 replicates, totaling 210 experimental units. Coffee seedlings showing brown eye spot presented reductions in the minimum water potential and in photosynthesis as a function of the lower irrigation level, except for the irrigation level 0.6 mm.day-1. The activity of the antioxidant system in diseased seedlings was only higher under higher irrigation levels. Results proved that the presence of the disease increased water stress in coffee seedlings for reducing both water potential and efficient water use.
DOI: 10.6084/m9.figshare.7941983.v1
title: Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats

description: We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets.
DOI: 10.6084/m9.figshare.7941971
title: Leptospirosis in Campinas, São Paulo, Brazil: 2007-2014

description: ABSTRACT: Objective: to analyze the distribution of leptospirosis according to sociodemographic, epidemiologic, and clinical characteristics, assistance time, rainfall and spatial distribution in Campinas/SP in the period from 2007 to 2014. Method: This is an ecological study using information from the confirmed cases. A descriptive analysis was carried out according to the selected variables. The relationship between the cases and rainfall was verified through the Spearman’s correlation coefficient. The cases/deaths were georeferenced per areas classified according to socioenvironmental deprivation indexes in Campinas. Results: From the 264 cases, 76.1% occurred in men, 58,4% in the 20 to 49 years old age range. Approximately 55% were hospitalized, with a prevalence of clinical-laboratorial diagnoses of 89.4%, and the lethality was 10.6%. The urban area concentrated 74.2% of the cases, and 48,9% occurred in the household environment. The main risk factor was evidence of the presence of rodents at the site. There was strong correlation between the incidence of cases and rainfall (p &lt; 0.05) in 2010 to 2012. A higher concentration of the cases was observed in the Center-Southwest region. Spatial distribution of cases/deaths showed clusters in regions classified at lower socioeconomic levels. Conclusions: The patterns of occurrence of leptospirosis in Campinas showed similarity with other Brazilian municipalities. The identification of areas with higher incidence of cases/deaths contributes to the adoption of strategies for intervention and prioritization of resources aiming at the reduction of the risk of infection and at early treatment for those affected.
DOI: 10.6084/m9.figshare.7941974.v1
title: Alternative treatments for the conservation of castor seeds (Ricinus communis L.)

description: ABSTRACT The aim of this study was to evaluate the effects of alternative treatments and storage environment on the physiological and sanitary qualities of castor seeds. Treatments were: derosal, vitavax-thiram, sodium benzoate, potassium sorbate, trichodermil SP, rosemary, garlic, cinnamon, Indian clove and control treatment. The seeds were subjected to storage in laboratory and in a cold chamber for 12 months. Castor seeds chemically treated with derosal and vitavax-thiram showed physiological and sanitary quality superior to those of seeds treated with alternative products and without treatment at 12 months of storage. Castor seeds stored in laboratory, for a period longer than 8 months, must be treated with traditional chemical fungicides. If the seeds are stored in a cold chamber, the maintenance of their germination and vigor above 80% does not depend on the used fungicide treatment. The most promising alternative fungicides were trichodermil, potassium sorbate and garlic, especially the first product.
DOI: 10.6084/m9.figshare.7941980.v1
title: Physiological analysis in coffee seedlings showing brown eye spot under different irrigation levels.

description: ABSTRACT Abstract: Brown eye spot is one of the major diseases that affect coffee seedling growth for causing defoliation and reducing the plant growth. The disease severity is higher when the coffee plant is subjected to water deficit. Using irrigation is an alternative to minimize the damage caused by this disease. The aim of this study was to evaluate the impact of this disease on the physiology of coffee plants as a function of the reduction in the irrigation level. The plants were subjected to three irrigation levels (0.1, 0.6 and 1.2 mm.day-1), inoculated and not inoculated with Cercospora coffeicola, in a completely randomized design with 35 replicates, totaling 210 experimental units. Coffee seedlings showing brown eye spot presented reductions in the minimum water potential and in photosynthesis as a function of the lower irrigation level, except for the irrigation level 0.6 mm.day-1. The activity of the antioxidant system in diseased seedlings was only higher under higher irrigation levels. Results proved that the presence of the disease increased water stress in coffee seedlings for reducing both water potential and efficient water use.
DOI: 10.6084/m9.figshare.7941962
title: Typology of dairy production systems that meet Brazilian standards for milk quality

description: ABSTRACT We aimed to compare the typology of dairy production systems (DPS) that meet Brazilian quality standards with that of non-compliant DPS. Semi-structured questionnaires were applied in 128 DPS located in Santa Izabel do Oeste, Paraná, Brazil (25°49'16" S and 53°29'04" W). In addition, milk quality reports of each DPS were analyzed. Dairy production systems were segregated into two groups: G1 – DPS that were in accordance with Brazilian regulations on milk quality, and G2 – DPS that did not comply with Brazilian regulations. Exploratory factor analysis was performed on structural, production, and technical variables. Two factors were defined: F1 – production scale and bargaining power, and F2 – autonomy and production control. Groups 1 and 2 were evaluated according to their structural, production, and technical characteristics as well as their F1 and F2 values. A small fraction (6.25%) of DPS met the minimum quality standards for milk. Dairy production systems that comply with quality regulations have larger production scale, higher productivity, and greater autonomy and control of milk production. Consequently, they have better bargaining power with the industry for the marketing of milk.
DOI: 10.6084/m9.figshare.7941965
title: The dramaturgy of experts in Brazilian regulatory science: the case of the National Technical Commission for Biosecurity

description: Abstract This article investigates the Brazilian National Technical Commission on Biosecurity, an agency empowered to regulate genetically modified organisms in the country, and presents an analysis of its performance based on Goffman’s interactionist approach. The commission is analyzed as if it were a theater in which different experts perform and act on stage, with an audience, a backstage area, and conflicts related to the roles represented. These strategies make it possible to understand the process of consolidating and stabilizing this commission as a decision-making body on genetically modified organisms in Brazil. The stabilization of the commission is associated with stage management that takes conflicts out of the public view and moves them behind the scenes, presenting decisions as free from political interference or personal interests.
DOI: 10.6084/m9.figshare.7941971.v1
title: Leptospirosis in Campinas, São Paulo, Brazil: 2007-2014

description: ABSTRACT: Objective: to analyze the distribution of leptospirosis according to sociodemographic, epidemiologic, and clinical characteristics, assistance time, rainfall and spatial distribution in Campinas/SP in the period from 2007 to 2014. Method: This is an ecological study using information from the confirmed cases. A descriptive analysis was carried out according to the selected variables. The relationship between the cases and rainfall was verified through the Spearman’s correlation coefficient. The cases/deaths were georeferenced per areas classified according to socioenvironmental deprivation indexes in Campinas. Results: From the 264 cases, 76.1% occurred in men, 58,4% in the 20 to 49 years old age range. Approximately 55% were hospitalized, with a prevalence of clinical-laboratorial diagnoses of 89.4%, and the lethality was 10.6%. The urban area concentrated 74.2% of the cases, and 48,9% occurred in the household environment. The main risk factor was evidence of the presence of rodents at the site. There was strong correlation between the incidence of cases and rainfall (p &lt; 0.05) in 2010 to 2012. A higher concentration of the cases was observed in the Center-Southwest region. Spatial distribution of cases/deaths showed clusters in regions classified at lower socioeconomic levels. Conclusions: The patterns of occurrence of leptospirosis in Campinas showed similarity with other Brazilian municipalities. The identification of areas with higher incidence of cases/deaths contributes to the adoption of strategies for intervention and prioritization of resources aiming at the reduction of the risk of infection and at early treatment for those affected.
DOI: 10.6084/m9.figshare.7941959
title: Tuberculosis and its associated factors in a city in the metropolitan region of Rio de Janeiro

description: ABSTRACT: Introduction: The occurrence of tuberculosis has been related to the spatial organization and improvement of the living conditions of the population. However, this relationship is not directly related and tuberculosis illness involves processes at different levels of organization. Method: An ecological study analyzing the relationship between living conditions and tuberculosis in the city of Niteroi, Brazil. Two indicators, socio-environmental and programmatic, were created by factor analysis and analyzed by regression in the period of 2008 to 2012. Thematic maps were constructed to examine the distribution pattern of the incidence rate and indicators in the city. Results: The results showed a direct and significant association of the two indicators with the incidence rate of tuberculosis. A one-unit higher the programmatic indicator was associated with a 7% higher incidence rate. The socio-environmental indicator was associated with a 27% higher tuberculosis rate. Discussion: The results of the present study were consistent with the direct relationship between tuberculosis and living conditions in the city of Niteroi. Conclusion: We can conclude that the dynamics of tuberculosis transmission in Niteroi can be explained by the occurrence of the disease in areas of consolidated social periphery and by the social vulnerability of specific groups.
DOI: 10.6084/m9.figshare.7941962.v1
title: Typology of dairy production systems that meet Brazilian standards for milk quality

description: ABSTRACT We aimed to compare the typology of dairy production systems (DPS) that meet Brazilian quality standards with that of non-compliant DPS. Semi-structured questionnaires were applied in 128 DPS located in Santa Izabel do Oeste, Paraná, Brazil (25°49'16" S and 53°29'04" W). In addition, milk quality reports of each DPS were analyzed. Dairy production systems were segregated into two groups: G1 – DPS that were in accordance with Brazilian regulations on milk quality, and G2 – DPS that did not comply with Brazilian regulations. Exploratory factor analysis was performed on structural, production, and technical variables. Two factors were defined: F1 – production scale and bargaining power, and F2 – autonomy and production control. Groups 1 and 2 were evaluated according to their structural, production, and technical characteristics as well as their F1 and F2 values. A small fraction (6.25%) of DPS met the minimum quality standards for milk. Dairy production systems that comply with quality regulations have larger production scale, higher productivity, and greater autonomy and control of milk production. Consequently, they have better bargaining power with the industry for the marketing of milk.
DOI: 10.6084/m9.figshare.7941965.v1
title: The dramaturgy of experts in Brazilian regulatory science: the case of the National Technical Commission for Biosecurity

description: Abstract This article investigates the Brazilian National Technical Commission on Biosecurity, an agency empowered to regulate genetically modified organisms in the country, and presents an analysis of its performance based on Goffman’s interactionist approach. The commission is analyzed as if it were a theater in which different experts perform and act on stage, with an audience, a backstage area, and conflicts related to the roles represented. These strategies make it possible to understand the process of consolidating and stabilizing this commission as a decision-making body on genetically modified organisms in Brazil. The stabilization of the commission is associated with stage management that takes conflicts out of the public view and moves them behind the scenes, presenting decisions as free from political interference or personal interests.
DOI: 10.6084/m9.figshare.7941950
title: Factors associated with obesity in urban collective transportation workers of the Metropolitan Region of Belo Horizonte, Minas Gerais, Brazil

description: ABSTRACT: Objective: To analyze the demographic, behavioral and occupational factors associated with obesity among urban collective transportation workers of the Metropolitan Region of Belo Horizonte, Minas Gerais State, Brazil. Method: This is a cross-sectional study conducted with 1,448 drivers and collectors in the Metropolitan Region of Belo Horizonte. Anthropometric, demographic, behavioral data, as well as participants’ link to the company and bus conditions were gathered in 2012 through a questionnaire applied by an interviewer. To calculate obesity, the body mass index cut off point was ≥ 30 kg/m2. Prevalence ratios (PR) and respective 95% confidence intervals (95%CI) were adjusted by Poisson’s multivariate regression. Results: The prevalence of obesity among urban collective transportation workers was 16.1%. Female sex (PR = 1.84; 95%CI 1.37 - 2.49), aging 30 to 39 years old (PR = 1.66; 95%CI 1.17 - 2.37) and 40 to 49 years old (PR = 1.59; 95%CI 1.04 - 2.42), being in the same job role from 5.01 to 10 years (PR = 1.52; 95%CI 1.04 - 2,42) and from 20.01 to 47 years (PR = 1.90; 95%CI 1.21 - 3.00), and physical inactivity (PR = 1.32; 95%CI 1.01 - 1.73) remained independently associated with obesity after multivariate adjustment data. Conclusion: These findings highlight the need to consider actions that encourage employees to participate in healthy activities when discussing health promotion for public transport workers, as well as actions to improve the organization and management of work, so it becomes a health and well-being feature for this population.
DOI: 10.6084/m9.figshare.7941953
title: Trend in mortality from preventable causes in children: contributions to the evaluation of the performance of public health services in the Southeast Region of Brazil

description: ABSTRACT: Objective: The aim of this study was to analyze the trend in mortality of children under 5 years old living in the Southeast Region of Brazil and states using the “Brazilian List of Causes of Preventable Deaths”. Method: We conducted an ecological time-series study of mortality from preventable and non-preventable causes, with corrections for ill-defined causes and underreporting of deaths, from 2000 to 2013. Results: There was a decline in the rate of childhood mortality due to preventable (4.4% per year) and non-preventable (1.9% per year) causes in the Southeast Region and its states, except for those reducible by vaccine prevention, which remained stable in the period. The study called attention to the smaller decrease in causes of preventable deaths by providing adequate care to women during pregnancy (1.7%), with an increase in mortality rates due to basic causes of death due to maternal conditions affecting the fetus or newborn and stability in disorders related to short-term pregnancy and low birth weight, a fact that possibly occurred due to inadequate quality of prenatal care. Minas Gerais showed the greatest reduction in annual percentage of deaths from preventable causes (5.5%), compared to other FUs, but it led in mortality rates up to 2010, while Rio de Janeiro led between 2010 and 2013. Conclusion: The decline in childhood mortality was expected in the last decade, due to progress in the response of health care systems, and to improvements in health and determinant social conditions as well. However, the rate is still high compared to other countries, showing that there is still much room for improvement.