cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5285/8caf2d8a-564d-4f2e-a797-174165a83796
title: The seed set of supplemented and pollinator exposed flowers from Eschscholzia californica plants located within habitats comprising different floral cover

description: This dataset details the number of seeds produced by pollinator exposed and supplemented Eschscholzia californica plants introduced to habitats comprising different floral cover. Data was collected in June 2015 at the Hillesden estate, Buckinghamshire, UK. Experimental arrays were introduced across the study site. Arrays comprised of three E.californica plants separated by 1m and arranged in a triangular formation. A total of sixteen arrays were introduced across four 100ha replicate blocks, each separated by >500m. At the centre of each block, four experimental arrays were placed at 50m intervals along a 150m transect laid symmetrically across the boundary between an established wildflower patch and bare, fallow ground or grazed grassland (two arrays within the florally rich habitat and two arrays within the florally poor habitat). After 16 days plants were collected in and one flower from each plant was supplemented with outcrossed pollen. Upon fruit maturation the mean number of seeds counted from pollinator exposed fruit were then compared to the number of seeds from supplemented fruit to determine the degree of pollen limitation in relation to habitat context. The dataset was part of a larger experiment looking at the effect of floral resources on the pollination services to isolated plants.
description: All open flowers were removed from the 48 E. californica plants prior to their placement in pre-marked locations across the landscape. Plants remained in the field for 16 days to ensure full anthesis of new flowers and to allow for multiple pollination events. After this period, we supplemented a flower from each of the 48 plants with outcrossed pollen. This involved methodically wiping four dehiscing anthers from a donor plant onto the receptive stigma of a pollinator exposed plant with dissecting tweezers. All fruit were then tagged and supplemented flowers were covered with fine muslin to protect against accidental windborne transfer of pollen from the glasshouse air-conditioning system. Plants were then collected and stored under controlled glasshouse conditions until fruit maturation. Upon maturation, tagged fruit were collected and the number of filled seeds per fruit were counted to quantify seed set per fruit. The degree of pollen limitation was expressed as a ratio between the mean seed set (field exposed plants) and the potential seed set (supplemented) in each of the 48 field exposed plants.
DOI: 10.5285/9da9bf05-fdbf-41f9-8591-1a4af9b84000
title: Population dynamics of evolving bacterial populations in soil, 2020

description: This dataset describes the composition of a co-evolving community (as colony forming units) over a 30-transfer selection experiment in soil microcosms. The community consists of Pseudomonas fluorescens SBW25 + Pseudomonas putida KT2440 + plasmid pQBR57. 
description: This is count data collected by plating bacterial populations on selective agar. File includes counts, dilution factors and volumes used to calculate density as colony forming units per ml (CFU/ml).
DOI: 10.5285/875ca233-ffaa-45c2-b4d2-db7d21bc0e61
title: Tensiometer measurements from a field experiment in the Conwy valley, North Wales, UK (2013 - 2015)

description: Data are presented of tensiometer measurements as centimetres of water from a field experiment in the Conwy catchment. The data were collected between October 2013 and January 2015 using tensiometers inserted into boreholes.

The data were collected by trained members of staff from Bangor University and the Centre for Ecology & Hydrology.

The samples were taken to provide supporting information for assessing the relationship between soil microbial populations and soil moisture status.

The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf.
description: Tensiometers were inserted vertically into holes bored using a hand auger. Soil water potential was measured using refillable Delta-T Devices type SWT4R tensiometers connected to a Delta-T logger. Data were regularly downloaded using Delta-T software to a laptop. 
Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC
DOI: 10.5285/be44b9c1-7355-4894-8f0d-b57b219b249b
title: Intergroup contests in banded mongooses (Mungos mungo) on the Mweya peninsula, Uganda, 2000-2019

description: These data are data on the outcomes of contests between groups of banded mongooses in Mweya Peninsula, Uganda. Data were collected between 2000 and 2019. The data include information on group membership (number and sex of individuals, age, etc…) as well as physiological data (weight), genetic data (pedigree), and behavioural data (contest outcomes, eviction). 
description: The data are collected by a team in Uganda and then transferred to researchers in the UK for filtering and organization.
DOI: 10.5285/1b5a8827-4329-4c20-85f8-0979998b3c74
title:  Adult and larvae mosquito counts from 12 UK wetland sites in southern England, collected between March 2017 and October 2018

description: This dataset contains a mosquito species table with counts for adults and larvae. Samples are from twelve UK wetland sites, sampled between April 2017 and September 2018. A map included in the documentation included with this data shows site locations, which include both coastal and inland wetlands, and range from Devon to Kent and from Lincolnshire to Dorset.

Samples were collected by staff from University of Greenwich and the UK Health Security Agency: collaborators in a NERC-funded project (NE/NO13379/1), part of the Valuing Nature Programme.
We found a total of 19 mosquito species:
•	10 Aedes
•	3 Anopheles
•	3 Culisseta
•	2 Culex
•	1  Coquillettidia
description: Twelve wetland sites in England were sampled, using Mosquito Magnet traps for adult mosquitoes and  water samples for larvae. An average of 9 samples are available for each of the twelve sites, with sampling dates ranging from April 2017 to September 2018.  All mosquitoes were identified down to species and counted.

Larval surveys were conducted, once each in May, July and September, across the range of different aquatic habitats found at each site. Larval samples were stored in 75% ethanol.

Two Mosquito Magnet® traps operated continuously at each site for four days nights every fortnight, from the first week of April to the third week of October. Adult traps help detect species missed in larval surveys and  assess the relative abundances of adult mosquitoes. The traps attract mosquitoes by mimicking host animals. Burning propane provides carbon dioxide and heat, and an octenol bait, found in cattle odour, acts as an attractant.

Adults and larvae were identified according to published keys, with secondary verification within the team. 

Attempts were made to sample across a range of habitats at each site. However landscapes varied, and strict comparability between sited cannot be guaranteed.

The data were gathered to help calibrate a prediction tool for people managing wetland sites.
DOI: 10.5285/2ea22f99-242c-4524-a5dc-3067a5f97a72
title: Chlorophyll absorbance data from lake and river samples incubated with different nutrient combinations in the English Lake District, Norfolk Broads and Greater Glasgow, 2017-2018

description: The dataset contains spectrophotometrically measured chlorophyll absorbance data from lake and river samples incubated with different nitrogen, phosphorus, silica or combined nutrients, as bioassays, either ex situ for phytoplankton or in situ for periphyton. These data were collected from sites in the English Lake District, Norfolk Broads and Greater Glasgow area in 2017 and 2018 as part of the NERC funded Freshwater Highlight Topic 'Hydroscape'.

The work was supported by the Natural Environment Research Council (Grant NE/N00597X/1).
description: Surface dip water samples were collected at the deepest point, lake edge, or ~1 m from the river bank. Periphyton sample filters were obtained from nutrient diffusing periphytometers that were deployed in the streams for two weeks. After two weeks these filters were retrieved and frozen at -20°C on return from the field. 

Phytoplankton bioassays were initially filtered through a 100 µm mesh, 3x35 ml of filtered water treatments were spiked with the different nutrient additions. Tubes were then incubated for 14 days at 20°C. Following the incubation period, the contents of the tubes were resuspended and filtered onto Whatman GF/C filters and frozen at -20°C.

Filters in all cases were defrosted and extracted in 7.36 ml hot methanol, with absorbance readings obtained from a Hitachi U2800 UV/Visible spectrophotometer using a 5cm cuvette.

Data were entered into a spreadsheet and manually checked for transcription errors. Data from all experiments were collated into a single file for data archiving purposes.
DOI: 10.5285/328cacca-c33b-450a-a39c-302b4eed07a2
title: Greenhouse gas and nutrient data measured across estuaries in the UK, 2017-2018

description: The dataset contains dissolved greenhouse and nutrient data collected from seven UK estuaries: the Clyde, Clywd, Conwy, Dart, Forth, Tamar and Tay, for the LOCATE project. Sampling was conducted from July 2017 to April 2018 on a quarterly basis, and was coordinated to occur on falling tides and to target specific salinities of interest: 1, 2, 5, 10, 15, 25 psu. Data were obtained via collection of gas and water samples in situ, and concentrations were derived via subsequent sample processing and analysis.  
description: All data were generated by the authors. During the generation of water chemistry and dissolved greenhouse gas data, calibration standards of known concentrations were used for quality control of instrument outputs.
DOI: 10.5285/2e0b5dac-651e-4026-8bd2-2a1da38606d6
title: The Isle of May long-term study (IMLOTS) seabird annual breeding success 1982-2024

description: This dataset contains calculated breeding success rates for six seabird species from representative colonies on the Isle of May, off the East coast of Scotland. Annual breeding success has been measured as the number of chicks fledged per active nest for the Atlantic puffin (Fratercula arctica, since 1982), common guillemot (Uria aalge, since 1982), razorbill (Alca torda, since 1982), European shag (Gulosus aristotelis, since 1987), black-legged kittiwake (Rissa tridactyla, since 1987) and northern fulmar (Fulmarus glacialis, since 1987).

This research was supported by NERC, through the UK Status, Change and Projections of the Environment (UK-SCAPE) programme NE/R016429/1 and the UKCEH National Capability for UK Challenges Programme NE/Y006208/1.
description: All fieldwork, sampling and data handling was carried out by experienced and trained staff to defined protocols agreed to meet the objectives of the work. All data was collected in the field in notebooks and field data sheets. These were transcribed to spreadsheets after each check was made and files backed up to UKCEH directories. As hatching and fledging dates were known the data recorded on spreadsheets were checked for inconsistencies. Any potential errors were then checked in the field. All notebooks and field sheets are archived at UKCEH Edinburgh.
DOI: 10.5285/7262ce48-0cc3-4998-89ee-36d676bd057d
title: Invertebrates identified in pitfall traps within diesel exhaust and ozone fumigation rings, Sonning, UK, 2018-2019

description: The dataset contains abundances of invertebrates collected from pitfall traps within eight field-based diesel exhaust and ozone fumigation rings located at the University of Reading’s Sonning Farm, UK. Rings were fumigated with diesel exhaust, ozone or both pollutants during two full summer seasons (April-October 2018 and 2019). Pitfall traps contained 500 mL 20% propylene glycol (including detergent to break the surface tension), which was filtered through mesh by two field researchers before collecting invertebrate samples within 50mL centrifuge tubes (one tube per pitfall trap) and placing in a -20 °C freezer prior to classification.
description: Invertebrates were collected from pitfall traps in field fumigation rings over two summer seasons (2018 and 2019) and taxonomically classified into functional groups and counted in the laboratory.
DOI: 10.5285/066b0d36-d28a-422e-b29a-298c98b8a536
title: Grassland productivity data across a gradient of invasive Pinus contorta abundance, northwest Patagonia, Argentina, 2020

description: This dataset contains total green biomass, palatable green biomass, sheep stocking rate, Pinus contorta tree density, P. contorta basal area and percentage canopy cover in sites across northwest Patagonia, during the summer of 2020. We measured total green biomass in the peak production (kg /hectare/year), as a metric for aboveground annual productivity, in five different sites and for a wide range of P. contorta abundance. We also measured palatable green biomass (kg/hectare/year), considering only those plant species foraged by sheep. We calculated the sheep stocking rate that can sustainably support the grasslands of our study based on the feeding requirement of an Ovine Livestock Unit (OLU). The OLU represents a Merino wether (castrated male sheep) with an average live weight of 40 kg that consume 365 kg of dry forage in a year in Patagonia grasslands. We counted the number of P. contorta individuals to calculate density (trees/ha) and recorded their diameter at ground level to calculate basal area (m2/ha) (an alternative measure of P. contorta abundance). Additionally, on each subplot we took a hemispheric picture to estimate the canopy cover (%) of P. contorta (a third measure of P. contorta abundance).
description: Field sampling: Green biomass of grassland grasses, forbs and shrubs was harvested during the summer of 2020 (January-February). Pinus contorta abundance was also measured during summer 2020. 
Laboratory measurements: Field samples were carried to the laboratory where they were oven dried at 60C for 48hs. Dried samples were weighed using a precision scale.
Computer analyses: All the data gathered was then filled into a spreadsheet and analysed.
DOI: 10.5285/67bbfabb-d981-4ced-b7e7-225205de9c96
title: Countryside Survey 1978 vegetation plot data

description: This dataset consists of plant species presence and abundance in different sizes and types of plots from 256 1km x 1km square sites surveyed across Great Britain in 1978. Many of the plots were repeated in 1990, 1998 and 2007. General information about the plot was recorded including plot number and type as well as species presence and (usually) cover. Data were collected under the Countryside Survey long term monitoring project managed by the Centre for Ecology & Hydrology. 
description: The data were recorded onto paper field sheets in the summer of 1978 by surveyors (a few sites were surveyed in 1977). Data were recorded from 256 1km squares sited across Britain, selected by random stratified sample. The survey was managed by the Institute of Terrestrial Ecology (which later became part of the Centre for Ecology & Hydrology). Standard Countryside Survey methodology was applied (refer to the online documentation).
DOI: 10.6084/m9.figshare.25511344
title: Supporting data for the manuscript: "Complete and stable enhanced biological phosphorus removal at 30 C using glutamate and acetate as alternating carbon sources"

description: Data and R scripts that support a study where we evaluated glutamate and acetate as alternate carbon sources for enhanced biological phosphorus removal (EBPR) in a laboratory-scale reactor operated at 30°C and surveyed the microbial community dynamics via 16S rRNA gene metabarcoding.
DOI: 10.6084/m9.figshare.25511344.v1
title: Supporting data for the manuscript: "Complete and stable enhanced biological phosphorus removal at 30 C using glutamate and acetate as alternating carbon sources"

description: Data and R scripts that support a study where we evaluated glutamate and acetate as alternate carbon sources for enhanced biological phosphorus removal (EBPR) in a laboratory-scale reactor operated at 30°C and surveyed the microbial community dynamics via 16S rRNA gene metabarcoding.
DOI: 10.5285/a65dbd74-c5b4-4c33-a658-9941cac0a049
title: Body size, lifespan, behaviour and body temperature in relation to environmental variables in wild field crickets (Gryllus campestris) in a meadow in Northern Spain, May and June, 2017

description: We provide data on how the body temperature of field crickets Gryllus campestris rises and falls in relation to exposure to the sun and environmental factors include ground and air temperature, diffuse and shortwave radiation, and wind speed. Crickets were in a meadow in N. Spain from May to June 2017.
Individual crickets were retained in a controlled area using a line attached to a copper ring glued to their thorax. Crickets were maintained in captivity and randomly selected every day to run the temperature increase trials. Trials were carried out in an area with mown grass. Before entering the trial, each individual was cooled to a temperature of 13ºC. During the cooling process, ambient temperature, humidity and reflected apparent temperature were measured using a thermal imagining camera (FLIR T4). Once the cricket reached the base temperature, it was released in the arena and its temperature measured at 1 minute intervals with the same camera, for a minimum of 20 minutes.
These data were acquired to assess behavioural thermoregulation in crickets in the wild and were collected by researchers at the University of Exeter.
description: Crickets in the body temperature dataset were collected in the wild in 2017 and then used to measure their body temperature changes with time under sun exposure. Crickets in the basic and behavioural traits datasets, were reared in captivity from parents collected at ten different populations, and then released in the study meadow after adult emergence. Data on body temperature increase were obtained from a sample of crickets collected in the wild and retained in a controlled area outside using a line attached to a copper ring glued to their thorax. Crickets were maintained in captivity and randomly selected every day to run the temperature increase trials. Trials were carried out in an area with mown grass. Before entering the trial, each individual was cooled to a temperature of 13ºC. During the cooling process, ambient temperature, humidity and reflected apparent temperature were measured using a thermal imagining camera (FLIR T4). Once the cricket reached the base temperature, it was released in the arena and its temperature measured at 1 minute intervals with the same camera, for a minimum of 20 minutes. Trial ended when the temperature plateaued and remained stable for approximately 2 minutes.
DOI: 10.5285/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7
title: Measurements of respiration, incorporation of litter carbon into soil fractions, and microbial community data using mineral-amended soils

description: This dataset includes measurements made during a laboratory-based study conducted between 10/2020 – 03/2021 on mineral amended soils with additions of two 13C labelled plant litters of contrasting quality. Data collected include: soil respiration (Carbon Dioxide (CO2))¬, total C content of bulk and soil fractions including mass and C recovery as a measure of the efficacy of the fractionation method, 13C enrichment of CO2 and soil fractions expressed as litter derived C, microbial data from bacterial (16S Small subunit ribosomal RNA) and fungal (internal transcribed spacer region 2) high throughput sequences. These data were collected to determine the relative effects of litter quality and soil mineralogy on the formation of soil C from plant material and may be useful to link plant inputs, soil microbes and soil mineralogy in the next generation of SOC models. Resolving the key fundamental mechanisms that control SOM persistence is crucial to optimise land management for SOC and predict soil C feedback to climate change. The experiment was part of the NERC project NE/S005137/2
description: Soil was collected from a commercial farm near Glassonby, Penrith and amended with pristine minerals (Kaolinite, Montmorillonite or Goethite) to create soil mineral treatments. Two 13C labelled litters were prepared in-house and added to soils to investigate the effect of litter quality and soil mineralogy on soil organic matter formation. The laboratory experiment was carried out at UK Centre for Ecology & Hydrology in Lancaster and soils were incubated under controlled conditions for 126 days. Respiration of litter-C was quantified by headspace CO2 and 13CO2 analysis on microcosms throughout the 126-day duration of the experiment and application of a two-component isotope mixing model. Concentrations of CO2 were analysed on a calibrated PerkinElmer Autosystem XL Gas Chromatograph. δ13C values of CO2 were analysed using a Picarro cavity ring-down spectrometer (model: G2201-I, Picarro, Inc. CA, USA). For enriched samples the instrument was calibrated for δ13C-CO2 using in-house prepared gas standards of 97.7 ‰ and 815 ‰. Litter carbon in soil fractions was determined by physicochemical density soil fractionation, and fractions analysed for C and δ13C using a Costech ECS-4010 Elemental Analyser (Costech Analytical Technologies, USA) coupled to a calibrated (cane sugar (−11.64 ‰), beet sugar (−26.03 ‰), enriched glucose (197.90 ‰) (IsoAnalytical, UK)) CRDS Picarro G-2131i isotopic analyser (Picarro Inc. CA, USA) and application of a two-component isotope mixing model.   Bacterial and fungal community compositions were assessed using 16S rRNA and ITS2 amplicon sequencing using the Illumina MiSeq platform with V2 500 cycle chemistry. Sequences were processed using the DADA2 pipeline to trim, quality-filter, de-noise, de-replicate, generate amplicon sequence variant tables and assign taxonomies.
DOI: 10.5285/7811769d-3e11-4b79-b781-765ca9cfd83e
title: Soil biogeochemical data from gradients of windthrow disturbance, Kielder Forest, England, summer 2022

description: This dataset contains soil biogeochemical data collected along gradients of windthrow disturbance in Kielder Forest, Northumberland, England, in summer 2022 as part of the Monitoring Underground Disturbance (MUD) BioBank. We established ~200 m transects at 10 sites (defined by common stand type, topography) and established 50m2 sampling plots in three different disturbance regimes across each transect. Soils were analysed for moisture content, organic carbon and nitrogen, and root biomass.
description: Samples were collected and analysed by L. Lancastle. We used a ThermoFlash Elemental Analyser to obtain carbon and nitrogen data, and root biomass was determined gravimetrically. Soil carbon and nitrogen content are recorded in units of percent dry mass, and root biomass data are reported in units of mg cm-3. Data were quality-checked and made available by B. Waring – all original observations are provided, no outliers have been deleted.