DOI: 10.5061/dryad.mw6m9062n
title: Data from: Testing the heat treatment dose for Agrilus planipennis prepupae using the Humble water bath

description: The lethal heat treatment dose (time and temperature) for the pre-pupal
 life stage of Agrilus planipennis Fairmaire (Coleoptera:
 Buprestidae), emerald ash borer, was determined through an in
 vitro application using a carefully calibrated heat
 treatment apparatus. The lethal and sublethal effects of heat on
 A. planipennis prepupae were assessed through a ramped heat
 delivery application, simulating industrial kilns and conventional heat
 chamber operations, for treatments combining target temperatures
 of 54 °C, 55 °C, and 56 °C, and exposure durations of 0 min
 (i.e., kiln temperature ramp only), 15 min, or 30 min. Prepupal
 emerald ash borer larvae did not survive exposure to 56 °C for 15
 min or longer, or to 55 °C for 30 min. Sublethal effects were observed
 for all other treatments. Sublethal effects included delayed
 development and failure to complete the pupal and adult life stages. The
 datasets and associated R statistical computing langauge code are
 deposited here.
description: The data are observations on post-treatment survival of emerald
 ash borer larvae exposed to heat in a water bath. The experiments were
 used to determine the optimal heating conditions for treatment of wood
 infested with the insect. The insects were exposed to one of three
 temperatures (54, 55 or 56 C) for a set period (0, 15 or 30 minutes) and
 then, along with unexposed control insects, were observed to determine if
 they died or transitioned to the next stage of development (i.e.,
 pupated).  The data were analysed with survival models to determine if
 temperature and duration of treatment had an effect on survival and the
 probability of pupating. Insects were observed every 24-72 h, pretreatment
 weight was also determined.
description: The associated analysis code was prepared in the R statistical
 computing language and require an instance of R to run. The
 'tidyverse', 'rms' and 'survminer' libraries
 are required in some of the R code files. Data are in .csv
 files.
description: # Data from: Testing the heat treatment dose for Agrilus planipennis
 prepupae using the Humble water bath Data are observations on
 post-treatment survival of emerald ash borer larvae exposed to heat in a
 water bath. The experiments were used to determine the optimal heating
 conditions for treatment of wood infested with the insect. The insects
 were exposed to one of three temperatures (54, 55 or 56 C) for a set
 period (0, 15 or 30 minutes) and then, along with unexposed control
 insects, were observed to determine if they died or transitioned to the
 next stage of development (i.e., pupated). The data were analysed with
 survival models to determine if temperature and duration of treatment had
 an effect on survival and the probability of pupating. ## Description of
 data and file structure There are 6 files in total: 1 ReadMe file, 2 data
 files and 3 associated code files (See Code/Software section). ReadMe.md:
 This file. heatTreat_larvaldata.csv: Records for each emerald ash borer
 larvae used in the experiments. These data were assembled from
 experimental notes and laboratory records held in .xls files. The data
 have been prepared in 'tidy' format after extraction from the
 original records. Description of fields: * \[]numeric; The row number for
 each record. * \[study] text; Either 'pilot study' or 'main
 study'; the experiment within which the data were collected (matches
 to 'Experiment 1' & 'Experiment 2' in the
 associated manuscript). * \[uniqueID] text; A unique ID code given to each
 individual. * \[treatment]text; Either 54, 55 or 56; the temperature to
 which each insect was treated. * \[replicateID text; Either A, B, or C;
 the code assigned to each replicate within an experiment. * \[duration]
 text; Either 0, 15, 30 or C; the length of time each insect was treated
 for. In the case of 'C' denotes the insect was in the unexposed
 control and was not exposed to the experimental conditions. * \[weight_g]
 double; Weight, in grams, of each insect before treatment. *
 \[larvaeAlive_10] binary (1,0); Denotes if the insect lived(1) or died(0)
 as a larvae after treatment. * \[pupalStage_10] binary (1,0); Denotes if
 the insect entered the pupal stage (1) or not (0) after treatment. *
 \[adultStage_10] binary (1,0); Denotes if the insect entered the adult
 stage (1) or not (0) after treatment (adult here meaning the insect took
 on the teneral form of EAB, see text of associated manuscript for further
 explanation). * \[cork] text; a temporary unique identifier assigned
 during treatment. This number was written on a cork that sealed the vial
 that contained the insect, hence the name (not recorded for insects in the
 pilot experiment and given as NA), not used in analyses. * \[bathPosition]
 text; The location in the Humble water bath where the insect was treated
 (not recorded for insects in the pilot experiment and given as NA), not
 used in analyses. * \[dishAndLarvae_weight_g] double; Weight, in grams, of
 the insect and the dish used to weigh it (not recorded for insects in the
 pilot experiment and given as NA), not used in analyses. * \[origin] text;
 A code combining the month and date insects were shipped, and the tray
 number the insects were shipped in (not recorded for insects in the pilot
 experiment and given as NA), not used in analyses. *
 \[larvaeLifespan_days] integer; Time each insect spent as a larvae after
 treatment, in days (not recorded in the raw data for insects in the pilot
 experiment and given as NA). * \[larvaeToPupae_days] integer; Time elapsed
 after treatment until insect pupated, in days (not recorded in the raw
 data for insects in the pilot experiment and given as NA). *
 \[pupaeToAdult_days] integer; Time elapsed after treatment until insect
 entered adult phase, in days (not recorded in the raw data for insects in
 the pilot experiment and given as NA). * \[totalLifespan_days] integer;
 insect's lifespan after treatment, in days. (not recorded in the raw
 data for insects in the pilot experiment and given as NA).
 heatTreat_observations.csv: Observation records of each emerald ash borer
 larvae after treatment and their ultimate fate. These data were assembled
 from experimental notes and laboratory records held in .xls files. The
 data have been prepared in 'tidy' format after extraction from
 the original data files. Description of fields: * \[]numeric; The row
 number for each record. * \[study] text; Either 'pilot study' or
 'main study'; the experiment within which the data were
 collected (matches to 'Experiment 1' & 'Experiment
 2' in the associated manuscript). * \[uniqueID] text; A unique ID
 code given to each individual. Allows records in this file to be cross
 referenced against the records for each experimental subject in
 'heatTreat_larvaedata.csv'. * \[timeOfObservation_hour] integer;
 Time, in hours, when insect was checked and its status assessed. *
 \[status] text; Either 0,1,2 or '-'; Code used to denote if
 insect evidenced movement (0); an injury (1) or was not moving (2) when
 checked; "-' denotes no observation at that time point. *
 \[notes] text; observations about each insect when it was checked,
 'NA' denotes no notes were recorded. ## Sharing/Access
 Information There are no other available versions of the data. ##
 Code/Software The data were analyzed using the R statistical computing
 environment and associated library packages authored for R. All analyses
 were done within the RStudio IDE. Associated files (not in repository)
 were used to generate figures in the associated manuscript.
 analysis_pretreatmentLarvalWeights.R: R statistical computing language
 script; Conducts an analysis of the weight of larvae in each replicate
 before treatment. Used to determine if weight was required as a covariate.
 See comments in file for more detail. Requires heatTreat_larvaldata.csv
 & the R package '`tidyverse`'. analysis_sampleSize.R: R
 statistical computing language script; Conducts an analysis of the sample
 size in each replicate. Used to compute summary statistics for associated
 manuscript. See comments in file for more detail. Requires
 heatTreat_larvaldata.csv & the R package '`tidyverse`'.
 analysis_survivalFollowingTreatment_probabilityOfPupation.R: R statistical
 computing language script; Conducts a Cox proportional hazards analysis of
 the larval survival data. For pilot experiment/experiment 1 also
 determines critical values for survival analysis from raw observations in
 heatTreat_observations.csv. See comments in file for more detail. Requires
 heatTreat_larvaldata.csv, heatTreat_observations.csv & the R packages
 '`tidyverse`','`rms`' & '`survminer`'.