DOI: 10.57760/sciencedb.12528
title: Thirty-three transcriptomes from eleven developmental periods using a tissue culture system of Bambusa odashimae
title: Thirty-three transcriptomes from eleven developmental periods using a tissue culture system of Bambusa odashimae

description: We collected shoots in the eleven periods/treatments (three biological replicates) during the tissue culture system. Total RNA was isolated with TRIzol reagent. Illumina sequencing libraries were generated using the standard protocol. The paired-end sequencing libraries were sequenced on an Illumina NovaSeq 6000 sequencer. Here is a brief introduction to the periods/treatments in this study.Shoot tips of the nodal explants were induced flowering in MS medium containing 0.1 mg/L TDZ (TMs). In this process, vegetative shoots emerged first, and later inflorescences gradually emerged. We collected 50-80 explants for in vitro flower induction every 21 days, and repeated eight times. After repeating eight times, we obtained in vitro shoots at different stages, including the vegetative stages (TM01-TM04), the stages of floral transition (TM05 and TM06), and the reproductive stages (TM07 and TM08). In vitro grown inflorescence clusters of B. odashimae (TM08) were transferred to MS medium containing 5 mg/L of NAA, and the vegetative shoots emerged. Continuously subcultured in this medium, floral tissue gradually disappeared and onlyvegetative tissue continued to grow, which means a transition from reproductive growth to vegetative growth. Placing vegetative shoots (TM04) in MS medium supplemented with auxin and cytokinin (2 mg/L 6- BenzylAdenine and 0.2 mg/L NAA) delayed flowering. Vegetative shoots of inflorescence origin could maintain vegetative growth in the MS medium without any plant growth regulators (CK) and had not bloomed for more than three years.
description: We collected shoots in the eleven periods/treatments (three biological replicates) during the tissue culture system. Total RNA was isolated with TRIzol reagent. Illumina sequencing libraries were generated using the standard protocol. The paired-end sequencing libraries were sequenced on an Illumina NovaSeq 6000 sequencer. Here is a brief introduction to the periods/treatments in this study.Shoot tips of the nodal explants were induced flowering in MS medium containing 0.1 mg/L TDZ (TMs). In this process, vegetative shoots emerged first, and later inflorescences gradually emerged. We collected 50-80 explants for in vitro flower induction every 21 days, and repeated eight times. After repeating eight times, we obtained in vitro shoots at different stages, including the vegetative stages (TM01-TM04), the stages of floral transition (TM05 and TM06), and the reproductive stages (TM07 and TM08). In vitro grown inflorescence clusters of B. odashimae (TM08) were transferred to MS medium containing 5 mg/L of NAA, and the vegetative shoots emerged. Continuously subcultured in this medium, floral tissue gradually disappeared and onlyvegetative tissue continued to grow, which means a transition from reproductive growth to vegetative growth. Placing vegetative shoots (TM04) in MS medium supplemented with auxin and cytokinin (2 mg/L 6- BenzylAdenine and 0.2 mg/L NAA) delayed flowering. Vegetative shoots of inflorescence origin could maintain vegetative growth in the MS medium without any plant growth regulators (CK) and had not bloomed for more than three years.