cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8031566.v1
title: Distance collaborative consultation in assistive technology for teachers: planning, implementation, and evaluation of a case study

description: Abstract The aim of this research was to plan, implement, and evaluate a distance collaborative consulting service in Assistive Technology (AT) for two teachers at the multifunctional resource room. This was a qualitative approach case-study, Three AT consultants also participated in the study in addition to the teachers. Data was collected by means of activities organized in the virtual learning environment and questionnaires. Initially, the data identified a demand for the teachers’ knowledge about AT. Next, the teachers chose a case of a student with cerebral palsy to receive support from the consultants. Finally, there was the evaluation of the service. The data were analyzed using a content analysis technique. The findings indicated that it is possible to identify the demands of the teachers regarding the use of AT at a distance, as well as to plan and implement collaborative consulting efforts. The contribution of this research is to indicate an alternative possibility for school support.
DOI: 10.6084/m9.figshare.8031560.v1
title: Dynamics of vegetation structure in a fragmented landscape in Minas Gerais, Brazil

description: Abstract Considering that area and edge effects are the most important factors that lead to landscape changes from the fragmentation of terrestrial communities, the present study aimed to analyze changes in the structure of vegetation classes of a fragmented landscape. The methodology employed was based on a model of patch dynamics for the years between 1979 and 2015. The analysis was performed with quantitative (area, shape and edge effect) and qualitative (low declivity of the terrain, fire resistance and tolerance to variation in light) variables of the classes of vegetation. Processes of retraction and expansion of the vegetation classes were identified, as well as the alteration of the structure of the fragments, which resulted in the intensification of the edge effect.
DOI: 10.6084/m9.figshare.8031548
title: Salicylic acid: resistance inducer to two-spotted spider mite in strawberry crop

description: ABSTRACT The strawberry is susceptible to several pests which cause a reduction in productivity. The use of mechanisms which repel or prevent these pests to establish, represent a sustainable environmental technology to reduce the frequency of agrochemical use. In this context, the aim of this study was to evaluate the effect of salicylic acid (SA) on resistance induction against two-spotted spider mite in strawberry cultivars (Aromas and Sweet Charlie). Five concentrations of SA (0, 25, 50, 75 and 100 mg L-1) were tested in order to verify the antixenosis effects. In SA concentration of 50 mg L-1, a reduced number of mites in the two strawberry cultivars was noticed. For the number of eggs deposited on leaflet, we verified an effect of SA concentration with quadratic adjustment in the equation. Sweet Charlie cultivar was more effective than Aromas in relation to the reduction of mite oviposition on leaflets treated with SA. SA concentrations of 25, 50 and 75 mg L-1 were the most efficient for the evaluated traits allowed to infer its potential as antixenotic resistance inducer against two-spotted spider mite in strawberry crop.
DOI: 10.6084/m9.figshare.8031548.v1
title: Salicylic acid: resistance inducer to two-spotted spider mite in strawberry crop

description: ABSTRACT The strawberry is susceptible to several pests which cause a reduction in productivity. The use of mechanisms which repel or prevent these pests to establish, represent a sustainable environmental technology to reduce the frequency of agrochemical use. In this context, the aim of this study was to evaluate the effect of salicylic acid (SA) on resistance induction against two-spotted spider mite in strawberry cultivars (Aromas and Sweet Charlie). Five concentrations of SA (0, 25, 50, 75 and 100 mg L-1) were tested in order to verify the antixenosis effects. In SA concentration of 50 mg L-1, a reduced number of mites in the two strawberry cultivars was noticed. For the number of eggs deposited on leaflet, we verified an effect of SA concentration with quadratic adjustment in the equation. Sweet Charlie cultivar was more effective than Aromas in relation to the reduction of mite oviposition on leaflets treated with SA. SA concentrations of 25, 50 and 75 mg L-1 were the most efficient for the evaluated traits allowed to infer its potential as antixenotic resistance inducer against two-spotted spider mite in strawberry crop.
DOI: 10.6084/m9.figshare.8031539
title: Reaction of strawberry cultivars to root-knot and root-lesion nematodes

description: ABSTRACT Considering the economic importance of strawberry in Brazil, with a clear migration trend from the soil to innovative systems on substrates, it is fundamental to evaluate the available cultivars in the market regarding tolerance to pathogens with potential for infestation. The reaction of eight commercial strawberry cultivars (Festival, Camino Real, Camarosa, Oso Grande, Monterey, San Andreas, Aromas, and Albion) to Meloidogyne and Pratylenchus species was evaluated. Strawberry cultivars were maintained in individual pots with sterilized soil and inoculated with 5,000 eggs + second stage juveniles of Meloidogyne arenaria, M. incognita, M. javanica or M. hapla or 1,000 specimens of Pratylenchus zea or P. brachyurus per plant. The experiment was carried out in a completely randomized design with six replications using tomato ‘Rutgers’ and sorghum ‘506’ plants as control to root-knot and lesion nematodes, respectively. Ninety days after inoculation, the nematode reproduction factor (RF= final population/initial population) was evaluated to determine the resistance of the strawberry genotypes to each nematode species. All cultivars behaved as resistant (FR&lt;1.00) or immune (FR= 0.00) to M. javanica, M. incognita, P. zeae and P. brachyurus. The cultivar ‘Camarosa’ was susceptible (FR&gt;1.00) to M. arenaria and M. hapla and ‘Oso Grande’ behaved as a good host to M. hapla; however, the other cultivars were resistant to these two Meloidogyne species. The assessed cultivars are poor hosts, being an alternative to be used in infested areas with these pests.
DOI: 10.6084/m9.figshare.8031542
title: Effect of dietary rapeseed and soybean oil on growth performance, carcass traits, and fatty acid composition of pigs

description: ABSTRACT The objectives of this investigation were to examine the impact of the dietary inclusion of rapeseed and soybean oil and the length of their feeding before slaughter on growth performance, quantitative and qualitative carcass traits, and fatty acid composition in longissimus lumborum muscle (LLM) of pigs. The experiment was conducted with 72 pigs (36 barrows and 36 gilts), divided into six oil-containing diet groups and one control group. Diets of the experimental groups were supplemented with 4% rapeseed (ROD) or soybean (SOD) oil for six, four, and two weeks before slaughter. Diet of the control group had no oil supplement. Animals were fed ad libitum and slaughtered at average body weight of 115.8 kg. The oil supplement and feeding duration had a significant effect on fatty acid composition in intramuscular fat of LLM. The diet with both rapeseed and soybean oil significantly increased the content of linoleic acid and α-linolenic acid (ALA) in intramuscular fat. Both types of oil significantly increased total PUFA, n-3 PUFA, and the PUFA:SFA ratio. The significant decrease of n-6:n-3 PUFA ratio was observed in groups fed rapeseed oil for four and six weeks. Dietary addition of oils did not have any significant effect on average daily gain, carcass traits, and physical characteristics of LLM of pigs. However, daily feed intake and feed conversion ratio were reduced in the groups with soybean oil supplement. The results show that rapeseed oil supplementation (two weeks before slaughter) has positive effect on n-6:n-3 PUFA ratio and increase of ALA without negative effect on meat and fat quality.
DOI: 10.6084/m9.figshare.8031530
title: Initial chest X-ray findings in pediatric patients diagnosed with H1N1 virus infection

description: Abstract Objective: To evaluate chest X-ray findings in pediatric patients diagnosed with influenza A (H1N1) virus infection. Materials and Methods: We retrospectively reviewed chest X-ray findings in 17 cases of pulmonary infection with the H1N1 virus (in 7 males and 10 females) examined between 2012 and 2016. The mean age of the patients was 14 months (range, 2-89 months). The diagnosis was established on the basis of clinical and radiographic criteria, and the virus was detected by polymerase chain reaction. The radiographic findings were categorized by type/pattern of opacity and by lung zone. The patients were divided into two groups: those not requiring ventilatory support; and those requiring ventilatory support or evolving to death. Results: The abnormality most often seen on chest X-rays was that of peribronchovascular opacities, the majority of which affected less than 25% of the lung, the involvement being bilateral and asymmetric. The lung zone most frequently involved was the middle third, with central and peripheral distribution, without pleural effusion. There was a statistically significant difference between the groups in terms of the symmetry of pulmonary involvement, asymmetric findings predominating in the group that required ventilatory support (p = 0.029). Conclusion: In pediatric patients with H1N1 virus infection, the main alterations on the initial chest X-rays are peribronchovascular opacities, nonspecific alveolar opacities, and consolidations. Although the definitive diagnosis of H1N1 virus infection cannot be made on the basis of imaging characteristics alone, using a combination of clinical and radiographic findings can substantially improve the diagnostic accuracy.
DOI: 10.6084/m9.figshare.8031539.v1
title: Reaction of strawberry cultivars to root-knot and root-lesion nematodes

description: ABSTRACT Considering the economic importance of strawberry in Brazil, with a clear migration trend from the soil to innovative systems on substrates, it is fundamental to evaluate the available cultivars in the market regarding tolerance to pathogens with potential for infestation. The reaction of eight commercial strawberry cultivars (Festival, Camino Real, Camarosa, Oso Grande, Monterey, San Andreas, Aromas, and Albion) to Meloidogyne and Pratylenchus species was evaluated. Strawberry cultivars were maintained in individual pots with sterilized soil and inoculated with 5,000 eggs + second stage juveniles of Meloidogyne arenaria, M. incognita, M. javanica or M. hapla or 1,000 specimens of Pratylenchus zea or P. brachyurus per plant. The experiment was carried out in a completely randomized design with six replications using tomato ‘Rutgers’ and sorghum ‘506’ plants as control to root-knot and lesion nematodes, respectively. Ninety days after inoculation, the nematode reproduction factor (RF= final population/initial population) was evaluated to determine the resistance of the strawberry genotypes to each nematode species. All cultivars behaved as resistant (FR&lt;1.00) or immune (FR= 0.00) to M. javanica, M. incognita, P. zeae and P. brachyurus. The cultivar ‘Camarosa’ was susceptible (FR&gt;1.00) to M. arenaria and M. hapla and ‘Oso Grande’ behaved as a good host to M. hapla; however, the other cultivars were resistant to these two Meloidogyne species. The assessed cultivars are poor hosts, being an alternative to be used in infested areas with these pests.
DOI: 10.6084/m9.figshare.8031542.v1
title: Effect of dietary rapeseed and soybean oil on growth performance, carcass traits, and fatty acid composition of pigs

description: ABSTRACT The objectives of this investigation were to examine the impact of the dietary inclusion of rapeseed and soybean oil and the length of their feeding before slaughter on growth performance, quantitative and qualitative carcass traits, and fatty acid composition in longissimus lumborum muscle (LLM) of pigs. The experiment was conducted with 72 pigs (36 barrows and 36 gilts), divided into six oil-containing diet groups and one control group. Diets of the experimental groups were supplemented with 4% rapeseed (ROD) or soybean (SOD) oil for six, four, and two weeks before slaughter. Diet of the control group had no oil supplement. Animals were fed ad libitum and slaughtered at average body weight of 115.8 kg. The oil supplement and feeding duration had a significant effect on fatty acid composition in intramuscular fat of LLM. The diet with both rapeseed and soybean oil significantly increased the content of linoleic acid and α-linolenic acid (ALA) in intramuscular fat. Both types of oil significantly increased total PUFA, n-3 PUFA, and the PUFA:SFA ratio. The significant decrease of n-6:n-3 PUFA ratio was observed in groups fed rapeseed oil for four and six weeks. Dietary addition of oils did not have any significant effect on average daily gain, carcass traits, and physical characteristics of LLM of pigs. However, daily feed intake and feed conversion ratio were reduced in the groups with soybean oil supplement. The results show that rapeseed oil supplementation (two weeks before slaughter) has positive effect on n-6:n-3 PUFA ratio and increase of ALA without negative effect on meat and fat quality.
DOI: 10.6084/m9.figshare.8031518
title: Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

description: Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.
DOI: 10.6084/m9.figshare.8031521
title: Effect of lncRNA HULC knockdown on rat secreting pituitary adenoma GH3 cells

description: Pituitary adenoma is one of the most common tumors in the neuroendocrine system. This study investigated the effects of long non-coding RNAs (lncRNAs) highly up-regulated in liver cancer (HULC) on rat secreting pituitary adenoma GH3 cell viability, migration, invasion, apoptosis, and hormone secretion, as well as the underlying potential mechanisms. Cell transfection and qRT-PCR were used to change and measure the expression levels of HULC, miR-130b, and FOXM1. Cell viability, migration, invasion, and apoptosis were assessed using trypan blue staining assay, MTT assay, two-chamber transwell assay, Guava Nexin assay, and western blotting. The concentrations of prolactin (PRL) and growth hormone (GH) in culture supernatant of GH3 cells were assessed using ELISA. The targeting relationship between miR-130b and FOXM1 was verified using dual luciferase activity. Finally, the expression levels of key factors involved in PI3K/AKT/mTOR and JAK1/STAT3 pathways were evaluated using western blotting. We found that HULC was highly expressed in GH3 cells. Overexpression of HULC promoted GH3 cell viability, migration, invasion, PRL and GH secretion, as well as activated PI3K/AKT/mTOR and JAK1/STAT3 pathways. Knockdown of HULC had opposite effects and induced cell apoptosis. HULC negatively regulated the expression of miR-130b, and miR-130b participated in the effects of HULC on GH3 cells. FOXM1 was a target gene of miR-130b, which was involved in the regulation of GH3 cell viability, migration, invasion, and apoptosis, as well as PI3K/AKT/mTOR and JAK1/STAT3 pathways. In conclusion, HULC tumor-promoting roles in secreting pituitary adenoma might be via down-regulating miR-130b, up-regulating FOXM1, and activating PI3K/AKT/mTOR and JAK1/STAT3 pathways.
DOI: 10.6084/m9.figshare.8031530.v1
title: Initial chest X-ray findings in pediatric patients diagnosed with H1N1 virus infection

description: Abstract Objective: To evaluate chest X-ray findings in pediatric patients diagnosed with influenza A (H1N1) virus infection. Materials and Methods: We retrospectively reviewed chest X-ray findings in 17 cases of pulmonary infection with the H1N1 virus (in 7 males and 10 females) examined between 2012 and 2016. The mean age of the patients was 14 months (range, 2-89 months). The diagnosis was established on the basis of clinical and radiographic criteria, and the virus was detected by polymerase chain reaction. The radiographic findings were categorized by type/pattern of opacity and by lung zone. The patients were divided into two groups: those not requiring ventilatory support; and those requiring ventilatory support or evolving to death. Results: The abnormality most often seen on chest X-rays was that of peribronchovascular opacities, the majority of which affected less than 25% of the lung, the involvement being bilateral and asymmetric. The lung zone most frequently involved was the middle third, with central and peripheral distribution, without pleural effusion. There was a statistically significant difference between the groups in terms of the symmetry of pulmonary involvement, asymmetric findings predominating in the group that required ventilatory support (p = 0.029). Conclusion: In pediatric patients with H1N1 virus infection, the main alterations on the initial chest X-rays are peribronchovascular opacities, nonspecific alveolar opacities, and consolidations. Although the definitive diagnosis of H1N1 virus infection cannot be made on the basis of imaging characteristics alone, using a combination of clinical and radiographic findings can substantially improve the diagnostic accuracy.
DOI: 10.6084/m9.figshare.8031518.v1
title: Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

description: Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.
DOI: 10.6084/m9.figshare.8031524
title: Yield, frying quality, plant vigor, and maturity of potato clones

description: ABSTRACT The objective of this research was to evaluate advanced potato clones for tuber yield, frying quality, plant vigor, and maturity, grown from minituber seeds, and to assess correlations between these traits. Eight advanced potato clones of Embrapa Potato Breeding Program (F11-09-03, F05-11-03, F54-11-06, F141-11-01, C2718-12-09, C2718-24-09, C2743-09-09, and CL308) and three commercial cultivars (Agata, Asterix and BRSIPR Bel) were evaluated. Experiments were carried out in autumn 2017, in Canoinhas-SC and Pelotas-RS. A randomized complete block design with four replicates was used. We used tuber seeds type IV (minibubers). We evaluated plant vigor, maturity, yield and number of marketable tubers, yield and number of total tubers, average tuber weight, specific gravity, and frying color. Individual and joint variance analyses revealed significant differences (p &lt;0.05) among genotypes for all traits at both locations. The GxE (genotype x environment) interaction was significant for all traits. For the two locations together, clones F05-11-03, C2718-12-09, C2718-24-09 and the control cultivar BRSIPR Bel showed higher marketable tuber yields. The first two clones also showed tubers with high average tuber weight. Clone F54-11-06 showed the later maturity at both locations. Regarding the frying quality characteristics, only clone F141-11-01 showed a pattern comparable to that of the control cultivar Asterix. Also, clones with higher plant vigor had higher tuber yield, but late maturity. Frying quality traits were not correlated between them and among other traits.