cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.25028171.v1
title: FBP.SBP KO in CMM with 13C-glycerol

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028180
title: FBP.SBP KO in HMI-9 with 13C-glycerol

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028180.v1
title: FBP.SBP KO in HMI-9 with 13C-glycerol

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028255
title: FBP.SBP KO in CMM medium

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028255.v1
title: FBP.SBP KO in CMM medium

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028204.v1
title: Phosphofructokinase RNAi

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028204
title: Phosphofructokinase RNAi

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028285
title: Transaldolase knock-out

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028285.v1
title: Transaldolase knock-out

description: <i>Trypanosoma brucei</i> is a causative agent of the Human and Animal African Trypanosomiases. The mammalian stage parasites infect various tissues and organs including the bloodstream, central nervous system, skin, adipose tissue and lungs. They rely on ATP produced in glycolysis, consuming large amounts of glucose, which is readily available in the mammalian host. In addition to glucose, glycerol can also be used as a source of carbon and ATP and as a substrate for gluconeogenesis. However, the physiological relevance of glycerol-fed gluconeogenesis for the mammalian-infective life cycle forms remains elusive. To demonstrate its (in)dispensability, first we must identify the enzyme(s) of the pathway. Loss of the canonical gluconeogenic enzyme, fructose-1,6-bisphosphatase, does not abolish the process hence at least one other enzyme must participate in gluconeogenesis in trypanosomes. Using a combination of CRISPR/Cas9 gene editing and RNA interference, we generated mutants for four enzymes potentially capable of contributing to gluconeogenesis: fructose-1,6-bisphoshatase, sedoheptulose-1,7-bisphosphatase, phosphofructokinase and transaldolase, alone or in various combinations. Metabolomic analyses revealed that flux through gluconeogenesis was maintained irrespective of which of these genes were lost. Our data render unlikely a previously hypothesised role of a reverse phosphofructokinase reaction in gluconeogenesis and preclude the participation of a novel biochemical pathway involving transaldolase in the process. The sustained metabolic flux in gluconeogenesis in our mutants, including a triple-null strain, indicates the presence of a unique enzyme participating in gluconeogenesis. Additionally, the data provide new insights into gluconeogenesis and the pentose phosphate pathway, and improve the current understanding of carbon metabolism of the mammalian-infective stages of <i>T. brucei</i>.
DOI: 10.6084/m9.figshare.25028309
title: ECOWAS Trade Data.xlsx

description: Data on my research on The impact of trade Integration on Poverty reduction in ECOWAS: A Quantile Regression Analysis
DOI: 10.1285/i22390359v59p1
title: Frontespizio
DOI: 10.1285/i22390359v59p3
title: Indice
DOI: 10.1285/i22390359v59p5
title: Studi - Articles
DOI: 10.1285/i22390359v59p7
title: A Critical Discourse Analysis of EU environmental education policies

description: In January 2022, the European Commission released a Proposal for a Council Recommendation on learning for environmental sustainability, in which it recognised that "[w]e are at a critical point in time: human action has brought us close to causing irreparable damage to our planet" and that "collective and individual actions are urgently needed to place our society and economy on a sustainable path" (European Commission, 2022, p. 1). The proposal was then adopted by the Council of the European Union in June 2022 with the "Council Recommendation on learning for the green transition and sustainable development" (Council of the EU, 2022), which focused on the key role of education in addressing the climate crisis and on the importance of preparing learners for the future. This chapter aims to critically analyse the aforementioned Commission Proposal and the ensuing Council Recommendation, as well as the Commission Staff Working Document accompanying the proposal (European Commission, Directorate-General for Education, Youth, Sport and Culture, 2022a), in order to discuss the main linguistic, pedagogical and political issues emerging from an ecocritically-informed analysis.
DOI: 10.1285/i22390359v59p23
title: La musa di Carlo Levi non si fermò a Eboli

description: This essay embraces the idea that Carlo Levi was a poet even before he was a painter or a prose writer. His masterpiece (Cristo si è fermato a Eboli), infact, could be read as an expandend prose poem by the archaic appearance. During the last two years of confinement in Lucania (from 1935 to 1936), Levi composed some poems in his notebook, later collected under the title Eterne lontananze. They represent some precious evidences of a real descent into an underworld out of history: warm testimonials that will be amplified, but also filtered in the Cristo, written after ten years. By commenting a selection of these poems, it's possible to point out themes, symbols, perspectives by which the author observed that reality, dominated by death and painted with black color, still putting trust in poetry as synonymous with freedom.
DOI: 10.1285/i22390359v59p39
title: Malpractice in online versus onsite computer-based language tests: Reflections from the COVID lockdown experience

description: Computer-based tests provide a fast, efficient and cost effective means of assessing students, especially if they can be administered online (Chalhoub-Deville 2002; Suvorov, Hegelheimer 2013). Traditionally, they are administered in controlled conditions, where supervision and invigilation (or proctoring) can be conducted in much the same way as it is with paper-based tests. Around the world, the COVID emergency (2020-21) led to the sudden closure of educational institutions and a shift online of all teaching and testing. In this paper, we will examine the effects of such a change in the context of two specific university courses where a set of bespoke Moodle-hosted computer-based (CB) tests were adapted from a controlled and supervised onsite context to a much freer online context using a general business communication platform (Microsoft Team). We compare the results of over 4,500 tests (Lexis and Use of English, Translation, Reading, Listening and Writing Tasks: the first four marked automatically, the last manually) taken in both onsite and online contexts to identify differences in results between both testing environments. The aim is to provide some hard data to ascertain how far online distance-invigilated tests taken under only the most basic supervision can be adequate substitutes for more traditional formats especially in view of both their positive aspects (e.g. greater accessibility and inclusivity for students) and their negative ones (e.g. the greater opportunity for malpractice).
DOI: 10.1285/i22390359v59p65
title: The trans-regeneration of Geordie into Albanian. A case study

description: In this article, we will introduce the concept of trans-regeneration: the way in which a traditional discourse (in this case a folk ballad) may be manifested as different texts as the story that it contains is told and retold both over time and over space. Our aim is to illustrate this process with the example of a well-known English-language ballad Geordie (of uncertain origins), and how, in its lifespan, it has undergone numerous changes in content both in the various English and non-English versions as it has been appropriated and performed by artists in many other languages. We will argue that, because of its distinct nature, it is useful to describe such a process with a distinct term, trans-regeneration, which differently to terms like transcreation, takes into account the important artistic considerations underlying the choices made by the various rewriters and performers. Finally, we will describe a case study where Albanian-speaking students participated in a workshop analysing the various trans-regenerations of Geordie based on Christiansen (2022) and were invited to produce their own version in Albanian, thereby becoming themselves not just spectators but also actors in this process.
DOI: 10.57745/fax3ta
title: FaceiraB_Thesis_101002admi202300549_ReadMe_20230803_V0.txt

description: Read Me file
DOI: 10.57745/j6h7t3
title: FaceiraB_Thesis_101002admi202300549_Fig1a_20230803_V0.jpg

description: Figure 1a
DOI: 10.57745/sqyzat
title: FaceiraB_Thesis_101002admi202300549_DatasFig1a_20230803_V0.opj

description: Data of cyclic voltammetry of Figure 1a