DOI: 10.5061/dryad.hf63h
title: Data from: Phylogeny of seven Bulinus species originating from endemic areas in three African countries, in relation to the human blood fluke Schistosoma haematobium

description: Background: Snails species belonging to the genus Bulinus (Planorbidae)
 serve as intermediate host for flukes belonging to the genus Schistosoma
 (Digenea, Platyhelminthes). Despite its importance in the transmission of
 these parasites, the evolutionary history of this genus is still obscure.
 In the present study, we used the partial mitochondrial cytochrome oxidase
 subunit I (cox1) gene, and the nuclear ribosomal ITS, 18S and 28S genes to
 investigate the haplotype diversity and phylogeny of seven Bulinus species
 originating from three endemic countries in Africa (Cameroon, Senegal and
 Egypt). Results: The cox1 region showed much more variation than the
 ribosomal markers within Bulinus sequences. High levels of genetic
 diversity were detected at all loci in the seven studied species, with
 clear segregation between individuals and appearance of different
 haplotypes, even within same species from the same locality. Sequences
 clustered into two lineages; (A) groups Bulinus truncatus, B. tropicus, B.
 globosus and B. umbilicatus; while (B) groups B. forskalii, B.
 senegalensis and B. camerunensis. Interesting patterns emerge regarding
 schistosome susceptibility: Bulinus species with lower genetic diversity
 are predicted to have higher infection prevalence than those with greater
 diversity in host susceptibility. Conclusion: The results reported in this
 study are very important since a detailed understanding of the population
 genetic structure of Bulinus is essential to understand the epidemiology
 of many schistosome parasites.
description: Cox1 Asmit region
 sequencesThis file covers all the
 cox1 (Asmit region) haplotypes for seven Bulinus species (B. truncatus, B.
 tropicus, B. globosus, B. umbilicatus, B. senegalensis, B. camerunensis
 and B. forskalii) collected from three African counties (Cameroon, Senegal
 and Egypt). They were sequenced using an ABI Prism BigDye® Terminator v1.1
 Cycle Sequencing Ready Reaction Kit (PE, Applied Biosystems), and were
 aligned using ClustalX in Mega6.Cox1-Asmit
 region.fstaCox1-Folmer region
 sequencesThis file covers all the
 cox1 (Folmer region) haplotypes for seven Bulinus species (B. truncatus,
 B. tropicus, B. globosus, B. umbilicatus, B. senegalensis, B. camerunensis
 and B. forskalii) collected from three African counties (Cameroon, Senegal
 and Egypt). They were sequenced using an ABI Prism BigDye® Terminator v1.1
 Cycle Sequencing Ready Reaction Kit (PE, Applied Biosystems), and were
 aligned using ClustalX in Mega6.Cox1-Folmer
 region.fsta18S-sequencesThis file covers all the 18S sequences for six Bulinus species (B. truncatus, B. tropicus, B. globosus, B. umbilicatus, B. senegalensis, and B. forskalii) collected from three African counties (Cameroon, Senegal and Egypt). They were sequenced using an ABI Prism BigDye® Terminator v1.1 Cycle Sequencing Ready Reaction Kit (PE, Applied Biosystems), and were aligned using ClustalX in Mega6.18s-sequences.fsta28S-sequencesThis file covers all the 28S sequences for six Bulinus species (B. truncatus, B. tropicus, B. globosus, B. umbilicatus, B. senegalensis, and B. forskalii) collected from three African counties (Cameroon, Senegal and Egypt). They were sequenced using an ABI Prism BigDye® Terminator v1.1 Cycle Sequencing Ready Reaction Kit (PE, Applied Biosystems), and were aligned using ClustalX in Mega6.