DOI: 10.5061/dryad.hdr7sqvt2
title: Data from: Exploring thermal tolerance across time and space in a tropical bivalve, Pinctada margaritifera

description: Ectotherm vulnerability to climate change is predicted to increase with
 temperature variation. Still, translating laboratory observations of
 organisms’ heat-stress responses to the natural fluctuating environment
 remains challenging. In this study, we used an integrative framework
 combining insights from thermal death time (TDT) curves and physiological
 reaction norms to precisely capture Pinctada margaritifera’s thermal
 performance and tolerance landscape. We then applied this integrative
 model to predict individuals’ cumulative heat injury as a function of
 actual temperature conditions documented at five contrasting islands
 across French Polynesia. Substantial injury was predicted for spats
 (ranging from 30.24% to 29.62%) when exposed to eight consecutive extreme
 low tide events in Nuku Hiva. Overall, this study highlights the potential
 of this framework to effectively quantify the impact of extreme events,
 such as marine heatwaves, and to guide resource management initiatives.
description: Three different experiments (E1, E2 and E3, details below) were
 performed using hatchery-produced individuals issued from two reproduction
 events. The E1 experiment aimed to assess larval development completion
 (i.e., performance and upper limits) under a wide range of temperatures
 and intensities (i.e. time of exposure).In turn, the E2 and E3 allowed
 investigating the thermal sensitivity of spats experiencing stressful and
 permissive temperatures, respectively. 
description: # Data from: Exploring thermal tolerance across time and space in a
 tropical bivalve, Pinctada margaritifera ## Description of the data and
 file structure **Results from E1 experiment** **#e1-binary** Column names
 indication: *tank* : Tank ID *replicate* : vial replicate ID *time* : time
 of exposure at the thermal condition (in *minutes*) *target_temp* :
 targeted temperature condition in the tank (in *degree Celsius*)
 *mean_temp* : mean measured temperature in the tank over the entire
 experiment duration (in *degree Celsius*) *sd_temp* : standard deviation
 of the measured temperaturein the tank over the entire experiment duration
 (in *degree Celsius*) *status* : status of each larva success, alive(1) /
 dead (0) **#e1-binomial** *success* : number of alive larva in the vial at
 Tfinal (k) *tot* : total number of embryo in the vial at Tinitial (n)
 *dead* : number of dead larva in the vial (n-k) *surv* : percentage of
 survival probability (k/n) **Results from E2 experiment** **#e2** Specific
 colnames indications *sp *: scientific name of the studied species
 *phylum *: phylum level of classification of the studied species *class *:
 class level of classification of the studied species *ID *: life stage
 identification  *date* : date of the experiment run *age *: age of the
 individuals (expressed in months) *mean_FW(g)* : mean fresh weight of the
 individuals *sd_FW(g)* : standard deviation of the fresh weight of the
 individuals *lifestage *: lifestage feature of the individuals *replicate
 :* ID of the replicated run *target_temp :* target temperature of exposure
 of the experimental tank (expressed in degree Celsius)  *mean_temp:* mean
 temperature of exposure  recorded in the experimental tank (expressed in
 degree Celsius)  *sd_temp *: standard deviation the temperature of
 exposure  recorded in the experimental tank (expressed in degree Celsius) 
 *adj_temp *: adjusted temperature of exposure  recorded in the
 experimental tank (round the value to the nearest whole number; expressed
 in degree Celsius)  LT50_min : time at which mortality in the tank reached
 50 % (in minutes) LOG_LT50 : log10 transformed LT50_min **Results from E3
 experiment** **#e3** Specific colnames indications: *measure* :
 physiological rate measured (respiration; ingestion, or scope for growth)
 *date* : the date on which the measurement was taken *replicate* : ID of
 the replicated run *tank* : ID of the experimental tank *target_temp :*
 target temperature of exposure of the experimental tank (expressed in
 degree Celsius)  *mean_temp:* mean temperature of exposure  recorded in
 the experimental tank (expressed in degree Celsius)  *sd_temp*: standard
 deviation the temperature of exposure  recorded in the experimental tank
 (expressed in degree Celsius)  *adj_temp*: adjusted temperature of
 exposure  recorded in the experimental tank (round the value to the
 nearest whole number; expressed in degree Celsius) *chamber* : ID
 microchamber used for physiological measurements *ind* : ID of the oyster
 *FW_tot* : Total fresh weight of the measured oyster (in *grams*)
 *FW_flesh* : Total fresh weight of the flesh of the measured oyster (in
 *grams*) *DW_flesh* : Total dry weight of the flesh of the measured oyster
 (in *grams*) *raw_rate* : Raw measured physiological rate (in mgO2/h/DW -
 for respiration; cell/h/DW - for ingestion; and J/h/DW - for scope for
 growth) *Ys* : raw-rate corrected with the allometric coefficient
 *rate_norm* : normalized Ys, by the control condition (i.e. 28°C) ## Codes
 A R code is available to reproduce analyses (*3.1. Estimating thermal
 thresholds*) done in the study. All data analyses and modelling were done
 using R version 4.3.1 (R Core Team, 2023). Thresholds of statistical
 significance (α) were set at 0.05, unless specifically stated.