cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14275547.v1
title: An integrative study of the invasive jumping-snail Ovachlamys fulgens (Gastropoda, Helicarionidae) in Rio de Janeiro and its fast spreading in Southeastern and Southern Brazil

description: Abstract The Japanese invasive jumping snail Ovachlamys fulgens is a pest of ornamental plants and an intermediate host of a nematode that causes eosinophilic meningitis. We expand its distribution to eight municipalities from Rio de Janeiro State, and one locality from the Paraná State, and generated for the first time partial sequences of the cytochrome c oxidase subunit I (COI) gene for Brazilian populations. External morphology, reproductive system, shell, radula, and jaw were also analyzed and described. Twenty-one lots were collected from Rio de Janeiro, Niterói, Magé, Miguel Pereira, Petrópolis, Teresópolis, Nova Friburgo, Bom Jardim and Paraty, in Rio de Janeiro State, and from Foz do Iguaçu, Paraná State. External morphology, shell and reproductive system were typical of O. fulgens, with some peculiarities found in the shell and radula. A single haplotype was found, which was 100% similar to sequences of COI available in GenBank for specimens from Japan and Argentina. The species seems to be adapted to many habitats and be rapidly expanding its distribution in Southeastern and Southern Brazil, and other South America countries. We highlight the importance of monitoring O. fulgens, considering its potential to compete with native mollusks, attack several plants, and be a transmitter of diseases.
DOI: 10.6084/m9.figshare.14275545.v1
title: Gradient analysis of landscape pattern in the core area of the “Zhengzhou and Kaifeng Integration” at the landscape level

description: Abstract The analysis of landscape pattern change is an effective way to understand urbanization. Research area is the core area of urbanization, which is between Zhengzhou city and Kaifeng city in China. Geographic information system (GIS) and landscape metrics method are used to quantify the gradient landscape pattern changes in the study area. The results show an obvious gradient change of landscape pattern from 2005 to 2015. The landscape pattern in the eastern and western of the study area experienced larger changes than the middle area. The western end mainly changed in terms of the patch number, patch shape and anthropogenic influence, but the patch types in the east changed more rapidly than those in the west. Landscape pattern change is influenced by policy and location, and human influence is the main driving force. Unlike previous study areas, in which urbanization expanded from the center of a city to its surroundings, our study area spread from two ends (Zhengzhou and Kaifeng cities) to the center of the study area. This study could be used as a typical case for the gradient analysis of landscape pattern changes during urbanization.
DOI: 10.6084/m9.figshare.14275548
title: Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique

description: Abstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.
DOI: 10.6084/m9.figshare.14275548.v1
title: Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique

description: Abstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.
DOI: 10.6084/m9.figshare.14275550
title: BRICS Astronomy and the United Nations Open Universe Initiative

description: Abstract The almost universal availability of electronic connectivity, portable devices, and the web is bringing about a major revolution: information of all kinds is rapidly becoming accessible to everyone, transforming social, economic and cultural life practically everywhere in the world. Internet technologies represent an unprecedented and extraordinary two-way channel of communication between producers and users of data. ”Open Universe” is an initiative proposed to the United Nations Committee on the Peaceful Uses of Outer Space (COPUOS) and currently in implementation under the leadership of the United Nations Office for Outer Space Affairs (UN-OOSA). Its primary objective is to stimulate a dramatic increase in the availability and usability of space science data, extending the potential of scientific discovery to new participants in all parts of the world. This paper describes the initiative in general, some of the activities carried out to demonstrate its feasibility, and its use in the context of the BRICS Astronomy Programme.
DOI: 10.6084/m9.figshare.14275550.v1
title: BRICS Astronomy and the United Nations Open Universe Initiative

description: Abstract The almost universal availability of electronic connectivity, portable devices, and the web is bringing about a major revolution: information of all kinds is rapidly becoming accessible to everyone, transforming social, economic and cultural life practically everywhere in the world. Internet technologies represent an unprecedented and extraordinary two-way channel of communication between producers and users of data. ”Open Universe” is an initiative proposed to the United Nations Committee on the Peaceful Uses of Outer Space (COPUOS) and currently in implementation under the leadership of the United Nations Office for Outer Space Affairs (UN-OOSA). Its primary objective is to stimulate a dramatic increase in the availability and usability of space science data, extending the potential of scientific discovery to new participants in all parts of the world. This paper describes the initiative in general, some of the activities carried out to demonstrate its feasibility, and its use in the context of the BRICS Astronomy Programme.
DOI: 10.6084/m9.figshare.14275553
title: Evaluation of different induced molting methods in Callinectes ornatus (Crustacea, Decapoda, Portunidae) as a tool for the commercial production of soft-shell crabs

description: Abstract We investigated the effects of eyestalk ablation and cheliped autotomy in inducing molting in Callinectes ornatus. The specimens in intermolt stage were divided into two size classes: 1 (30-50 mm) and 2 (51-70 mm) and were further divided into four experimental groups. In the CA group (Cheliped Autotomy, n=76), crabs were submitted to cheliped autotomy; in the UA group (Unilateral Ablation, n=66) and BA group (Bilateral Ablation, n=66) to unilateral and bilateral eyestalk ablation, respectively. The C group (Control, n=70) was used as control. The animals were individually kept in tanks interconnected to a recirculation system for 30 days. The highest frequency of premolt was recorded in the BA group (32%), followed by the CA group (16%). The premolt frequency of class 1 (28%) was significantly higher (p&lt;0.05) than of class 2 (8%). The mean time until molt in the BA group was significantly lower (p &lt;0.05) than other groups tested. The highest mortality rates were 55% and 25% in crabs from the BA and CA groups, respectively. The results indicate bilateral eyestalk ablation and cheliped autotomy are potentially capable of inducing molt in C. ornatus, but these techniques have limited efficiency for the commercial application of large-scale soft-shell crab.
DOI: 10.6084/m9.figshare.14268182
title: Phylogenomic analyses reveals gene flow between populations of the freshwater shrimp Potimirim brasiliana (Caridea, Atyidae) along its wide distribution

description: Abstract Potimirim is one of the 40 genera of Atyidae restricted to America, which occurs in coastal freshwater habitats and questions about population status and variability have been emerging. Potimirim brasiliana occurs in Brazil from the northeastern to southeastern region. In order to evaluate the hypothesis of genetic structure among populations, we performed molecular analyses with specimens from all known limit of distribution of the species. The molecular markers used were COI and 28S. Phylogenetic trees were obtained by maximum likelihood and Bayesian analyses, and a haplotype network was obtained based only on COI. We found clear separation between P. brasiliana, P. potimirim, P. glabra and Potimirim sp 2. No pattern of structuration was found among P. brasiliana, but the haplotype network showed geographic pattern of structuration for the congener P. potimirim. The lack of genetic structuration among P. brasiliana can be explained by its life cycle that requires brackish water to complete their larval development. The larvae and juvenile in contact with these habitats can spread through oceanic currents, especially in higher rainfall seasons, maintaining the gene flow. The explanation for the geographical pattern found among P. potimirim is still missing and aspects about its lifecycle and larval development should be investigated.
DOI: 10.6084/m9.figshare.14268182.v1
title: Phylogenomic analyses reveals gene flow between populations of the freshwater shrimp Potimirim brasiliana (Caridea, Atyidae) along its wide distribution

description: Abstract Potimirim is one of the 40 genera of Atyidae restricted to America, which occurs in coastal freshwater habitats and questions about population status and variability have been emerging. Potimirim brasiliana occurs in Brazil from the northeastern to southeastern region. In order to evaluate the hypothesis of genetic structure among populations, we performed molecular analyses with specimens from all known limit of distribution of the species. The molecular markers used were COI and 28S. Phylogenetic trees were obtained by maximum likelihood and Bayesian analyses, and a haplotype network was obtained based only on COI. We found clear separation between P. brasiliana, P. potimirim, P. glabra and Potimirim sp 2. No pattern of structuration was found among P. brasiliana, but the haplotype network showed geographic pattern of structuration for the congener P. potimirim. The lack of genetic structuration among P. brasiliana can be explained by its life cycle that requires brackish water to complete their larval development. The larvae and juvenile in contact with these habitats can spread through oceanic currents, especially in higher rainfall seasons, maintaining the gene flow. The explanation for the geographical pattern found among P. potimirim is still missing and aspects about its lifecycle and larval development should be investigated.
DOI: 10.6084/m9.figshare.14275551
title: Search and study of the space debris and asteroids within ISON project

description: Abstract International Scientific Optical Network (ISON) is an open international voluntary project specializing in observations of the near-Earth space objects. Observatories collaborating with ISON provide the global coverage and successfully combine the observations of the space debris and asteroids. The network includes more than 50 telescopes of 27 observatories in 15 countries and has been working since 2005. ISON monitors the whole GEO region and tracks the objects at GEO, GTO, HEO and LEO. ISON data allowing maintenance of the database of the space objects orbits, validating space debris population model and providing conjunction assessment analysis for satellites at high orbits. ISON develops the technology of asteroid survey with small telescopes and arranges regular photometry observations of near-Earth asteroids (NEA) to investigate the YORP effect, search new binary NEAs, and support radar experiments.
DOI: 10.6084/m9.figshare.14275551.v1
title: Search and study of the space debris and asteroids within ISON project

description: Abstract International Scientific Optical Network (ISON) is an open international voluntary project specializing in observations of the near-Earth space objects. Observatories collaborating with ISON provide the global coverage and successfully combine the observations of the space debris and asteroids. The network includes more than 50 telescopes of 27 observatories in 15 countries and has been working since 2005. ISON monitors the whole GEO region and tracks the objects at GEO, GTO, HEO and LEO. ISON data allowing maintenance of the database of the space objects orbits, validating space debris population model and providing conjunction assessment analysis for satellites at high orbits. ISON develops the technology of asteroid survey with small telescopes and arranges regular photometry observations of near-Earth asteroids (NEA) to investigate the YORP effect, search new binary NEAs, and support radar experiments.
DOI: 10.6084/m9.figshare.14275553.v1
title: Evaluation of different induced molting methods in Callinectes ornatus (Crustacea, Decapoda, Portunidae) as a tool for the commercial production of soft-shell crabs

description: Abstract We investigated the effects of eyestalk ablation and cheliped autotomy in inducing molting in Callinectes ornatus. The specimens in intermolt stage were divided into two size classes: 1 (30-50 mm) and 2 (51-70 mm) and were further divided into four experimental groups. In the CA group (Cheliped Autotomy, n=76), crabs were submitted to cheliped autotomy; in the UA group (Unilateral Ablation, n=66) and BA group (Bilateral Ablation, n=66) to unilateral and bilateral eyestalk ablation, respectively. The C group (Control, n=70) was used as control. The animals were individually kept in tanks interconnected to a recirculation system for 30 days. The highest frequency of premolt was recorded in the BA group (32%), followed by the CA group (16%). The premolt frequency of class 1 (28%) was significantly higher (p&lt;0.05) than of class 2 (8%). The mean time until molt in the BA group was significantly lower (p &lt;0.05) than other groups tested. The highest mortality rates were 55% and 25% in crabs from the BA and CA groups, respectively. The results indicate bilateral eyestalk ablation and cheliped autotomy are potentially capable of inducing molt in C. ornatus, but these techniques have limited efficiency for the commercial application of large-scale soft-shell crab.
DOI: 10.6084/m9.figshare.14275556
title: Intelligent Supernovae Classification Systems in the KDUST context

description: Abstract With the advent of large astronomical surveys plus multi-messenger astronomy, both automatic detection and classification of Type Ia supernovae have been addressed by different machine learning techniques. In this article we present three solutions aimed at the future spectrometer of the KDUST project, within a scope of benchmark, considering three different methodologies. The systems presented here are the following: CINTIA (based on hierarchical neural network architecture), SUZAN (which incorporates the solution known as fuzzy systems) and DANI (based on Deep Learning with Convolutional Neural Networks). The characteristics of the systems are presented and the benchmark is performed considering a data set containing 15.134 spectra. The best performance is obtained by the DANI architecture which provides 96% accuracy in the classification of Type Ia supernovae in relation to other spectral types.
DOI: 10.6084/m9.figshare.14275557
title: Microsatellite Markers for Bearded Capuchins (Sapajus libidinosus): Transferability and Characterization

description: Abstract Natural Sapajus libidinosus populations are in continuous decline due to fragmentation, habitat loss, and the illegal pet trade. They live in Caatinga scrub forests, which already lost over 50% of their original cover. The lack of studies on S. libidinosus population genetics means that we do not know how they are being affected by this striking habitat loss and other anthropogenic disturbances. Polymorphic markers are not available for the study of S. libidinosus diversity and population genetics. Thus, here we aimed to test the transferability of 14 microsatellite markers to S. libidinosus. These microsatellites were previously isolated from Cebus capucinus (white-faced capuchin), species belonging to the same subfamily (Cebinae) as the study species. We found that six of the tested microsatellite markers (tetra-nucleotide) were cross-amplified in our target species. All loci were polymorphic. The number of alleles varied from 4 to 7, and the expected heterozygosity ranged from 0.588 to 0.869. The microsatellite markers transferred to S. libidinosus and characterised in our study will be valuable tools to evaluate the genetic variability of both wild and captive populations. They will considerably reduce the costs of microsatellite isolations, helping to prioritise currently limited research and conservation budgets in Brazil.
DOI: 10.6084/m9.figshare.14275557.v1
title: Microsatellite Markers for Bearded Capuchins (Sapajus libidinosus): Transferability and Characterization

description: Abstract Natural Sapajus libidinosus populations are in continuous decline due to fragmentation, habitat loss, and the illegal pet trade. They live in Caatinga scrub forests, which already lost over 50% of their original cover. The lack of studies on S. libidinosus population genetics means that we do not know how they are being affected by this striking habitat loss and other anthropogenic disturbances. Polymorphic markers are not available for the study of S. libidinosus diversity and population genetics. Thus, here we aimed to test the transferability of 14 microsatellite markers to S. libidinosus. These microsatellites were previously isolated from Cebus capucinus (white-faced capuchin), species belonging to the same subfamily (Cebinae) as the study species. We found that six of the tested microsatellite markers (tetra-nucleotide) were cross-amplified in our target species. All loci were polymorphic. The number of alleles varied from 4 to 7, and the expected heterozygosity ranged from 0.588 to 0.869. The microsatellite markers transferred to S. libidinosus and characterised in our study will be valuable tools to evaluate the genetic variability of both wild and captive populations. They will considerably reduce the costs of microsatellite isolations, helping to prioritise currently limited research and conservation budgets in Brazil.
DOI: 10.6084/m9.figshare.14268308
title: Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid

description: Abstract Autism spectrum disorder is associated with alterations in GABAergic and glutamatergic neurotransmission. Here, we aimed to determine the concentration of GABA, glutamate, glutamine, aspartate, taurine, and glycine in brain tissue and plasma of rats prenatally exposed to valproic acid (VPA), a well-characterized experimental model of autism. Pregnant rats were injected with VPA (600mg/Kg) during the twelfth-embryonic-day. Control rats were injected with saline. On the fourteen-postnatal-day, rats from both groups (males and females) were anesthetized, euthanized by decapitation and their brain dissected out. The frontal cortex, hippocampus, amygdala, brain stem and cerebellum were dissected and homogenized. Homogenates were centrifuged and supernatants were used to quantify amino acid concentrations by HPLC coupled with fluorometric detection. Blood samples were obtained by a cardiac puncture; plasma was separated and deproteinized to quantify amino acid concentration by HPLC. We found that, in VPA rats, glutamate and glutamine concentrations were increased in hippocampus and glycine concentration was increased in cortex. We did not find changes in other regions or in plasma amino acid concentration in the VPA group with respect to control group. Our results suggest that VPA exposure in utero may impair inhibitory and excitatory amino acid transmission in the infant brain.
DOI: 10.6084/m9.figshare.14275556.v1
title: Intelligent Supernovae Classification Systems in the KDUST context

description: Abstract With the advent of large astronomical surveys plus multi-messenger astronomy, both automatic detection and classification of Type Ia supernovae have been addressed by different machine learning techniques. In this article we present three solutions aimed at the future spectrometer of the KDUST project, within a scope of benchmark, considering three different methodologies. The systems presented here are the following: CINTIA (based on hierarchical neural network architecture), SUZAN (which incorporates the solution known as fuzzy systems) and DANI (based on Deep Learning with Convolutional Neural Networks). The characteristics of the systems are presented and the benchmark is performed considering a data set containing 15.134 spectra. The best performance is obtained by the DANI architecture which provides 96% accuracy in the classification of Type Ia supernovae in relation to other spectral types.