cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.6ztdd7
title: Occurrence Download

description: A dataset containing 22 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Zornia grandiflora Fort.-Perez & A.M.G.Azevedo"
 ]
}

 The dataset includes 22 records from 10 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020443-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5287/ora-mpankr2oj
title: Accurate grain boundary plane distributions for textured microstructures from stereological analysis of orthogonal two-dimensional electron backscatter diffraction orientation maps - dataset

description: Raw data exported from the DREAM.3D software from synthetic microstructures created in this project to investigate a stereological method for obtaining grain boundary plane distributions (GBPD) for textured materials. For each synthetic microstructure, the dataset contains planar sections, emulating electron backscatter diffraction (EBSD) measurements, which were used for calculating the GBPD using stereology. The dataset also contains triangular meshes of the grain boundary network in 3D, which enabled accessing the ground truth GBPD, without the need for stereology. The data was generated between July 2022 and May 2025.
DOI: 10.15468/dl.bxun5s
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasGeospatialIssue is false",
 "HasCoordinate is true",
 "OccurrenceStatus is Present",
 "BasisOfRecord is one of (Human Observation, Machine Observation, Occurrence evidence, Observation)",
 "TaxonKey is one of (Circus hudsonius (Linnaeus, 1766))",
 "Country is one of (Algeria, Angola, Benin, Botswana, British Indian Ocean Territory, Burkina Faso, Burundi, Cabo Verde, Cameroon, Central African Republic, Chad, Comoros, Congo, Congo, Democratic Republic of the, Côte d’Ivoire, Djibouti, Egypt, Equatorial Guinea, Eritrea, Eswatini, Ethiopia, French Southern Territories, Gabon, Gambia, Ghana, Guinea, Guinea-Bissau, Kenya, Lesotho, Liberia, Libya, Madagascar, Malawi, Mali, Mauritania, Mauritius, Mayotte, Morocco, Mozambique, Namibia, Niger, Nigeria, Réunion, Rwanda, Saint Helena, Ascension and Tristan da Cunha, Sao Tome and Principe, Senegal, Seychelles, Sierra Leone, Somalia, South Africa, South Sudan, Sudan, Tanzania, United Republic of, Togo, Tunisia, Uganda, Western Sahara, Zambia, Zimbabwe)"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020444-250525065834625/datasets/export for details.
DOI: 10.15468/dl.s24anw
title: Occurrence Download

description: A dataset containing 68 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "Country is Brazil",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "TaxonKey is Zornia harmsiana Standl."
 ]
}

 The dataset includes 68 records from 28 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020446-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.fmqgfh
title: Occurrence Download

description: A dataset containing 3254 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Etlingera elatior (Jack) R.M.Sm."
 ]
}

 The dataset includes 3254 records from 134 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020445-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.kmgyfn
title: Occurrence Download

description: A dataset containing 34585 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Rhizophora mangle L."
 ]
}

 The dataset includes 34585 records from 399 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0020447-250525065834625/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.3pa64r
title: Occurrence Download

description: A dataset containing 183 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Ecuador",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "PublishingOrg is one of (bc092ff0-02e4-11dc-991f-b8a03c50a862, 90fd6680-349f-11d8-aa2d-b8a03c50a862, 8e1a97a0-3ca8-11d9-8439-b8a03c50a862, 33d232b5-5cc2-4521-8bd6-121c5bb41c32, 9661d20d-86b6-4485-8948-f3c86b022fa7, 6ba9a8cc-513a-4a51-bf93-6f5de8040a96, ae447c50-b8a8-11d8-92a4-b8a03c50a862, 061b4f20-f241-11da-a328-b8a03c50a862)",
 "TaxonKey is Calceolaria tripartita"
 ]
}

 The dataset includes 183 records from 9 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000294-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.7rxf6b
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Rhopophilus albosuperciliaris",
 "Country is Chinese Taipei",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0000292-250827131500795/datasets/export for details.
DOI: 10.22002/7z88b-4ga06
title: Myxococcus xanthus DK1622 wt

description: Imaged by UCLA Titan Krios.
description: <strong>Tilt Series Date:</strong> 2013-03-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> FEIbatchtomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> FEIBatchTomo</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-03-27-5/rawdata/</p>DK1622_UCLA_20130327_14.mrc,  Tilt Series (Pixel Size 1.352 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-03-27-5/rawdata/DK1622_UCLA_20130327_14.mrc"
        > <i class="download icon"></i> Download </a></p>

        DK1622_UCLA_20130327_14_full.rec,  Reconstruction (Pixel Size 2.704 nm), 838.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-03-27-5/3dimage_26039/DK1622_UCLA_20130327_14_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/nxbg7-xdr12
title: Hylemonella + Bdellovibrio

description: Raw data files of Hylemonella + Bdellovibrio
description: <strong>Tilt Series Date:</strong> 2013-01-03</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Hylemonella grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, incubated with attack-phase Bdellovibrio for 3 days, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2013-01-03-28_slicer6077.jpg: chemoreceptor array ycw2013-01-03-28_slicer6080.jpg: flagellar motor ycw2013-01-03-28_slicer6082.jpg: unknown filaments
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-28/rawdata/</p>Bdel_Hyle_0010004.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-28/rawdata/Bdel_Hyle_0010004.mrc"
        > <i class="download icon"></i> Download </a></p>

        Bdel_Hyle_0010004_full.rec,  Reconstruction (Pixel Size 1.6 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-01-03-28/3dimage_24307/Bdel_Hyle_0010004_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vgq0f-rj460
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC115
description: <strong>Tilt Series Date:</strong> 2014-09-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 3 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Wait time 10 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-43/rawdata/</p>20140915_YWC115_30036.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-43/rawdata/20140915_YWC115_30036.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140915_YWC115_30036_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-43/3dimage_46313/20140915_YWC115_30036_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ykcjz-7b476
title: Agrobacterium tumefaciens C1

description: wild type, no T4SS
description: <strong>Tilt Series Date:</strong> 2014-02-03</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown in liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
  etl2014-02-03-5_slicer7406.jpg: Type 4 secretion system .  T4SS etl2014-02-03-5_slicer7409.jpg: Periplasmic vesicle etl2014-02-03-5_slicer7410.jpg: Flat vesicles
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-03-5/rawdata/</p>VG_Plasmid0001.mrc,  Tilt Series (Pixel Size 0.4 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-03-5/rawdata/VG_Plasmid0001.mrc"
        > <i class="download icon"></i> Download </a></p>

        VG_Plasmid0001_part121_27.rec,  Reconstruction (Pixel Size 1.6 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-02-03-5/3dimage_38318/VG_Plasmid0001_part121_27.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kr9hg-rgj79
title: E.cloacae-typeV-CdiA+

description: Type V secretion system
description: <strong>Tilt Series Date:</strong> 2014-03-03</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Drs. Davod Low and Chris Hayes, UC Santa Barbara</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning</p>
<strong>Sample Preparation:</strong> Blot time 3s, Blot off-set -4, 3 ul culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-20/rawdata/</p>atlas10020.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-20/rawdata/atlas10020.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10020_full.rec,  Reconstruction (Pixel Size 1.92 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-20/3dimage_39551/atlas10020_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xaadd-72x53
title: Myxococcus xanthus JK328

description: Bactofilin BacMNOP knockout strain. Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-07-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
  ycw2013-07-22-60_slicer9139.jpg: Myxococcus Xanthus .  filament bundle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-60/rawdata/</p>07222013_Janelia_Myxo_JK328_20002.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-60/rawdata/07222013_Janelia_Myxo_JK328_20002.mrc"
        > <i class="download icon"></i> Download </a></p>

        07222013_Janelia_Myxo_JK328_20002_full.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-60/3dimage_28552/07222013_Janelia_Myxo_JK328_20002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/daz2q-1zw35
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug).
description: <strong>Tilt Series Date:</strong> 2012-11-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: None μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFTomo</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-2/rawdata/</p>12nov29_C129_1_pnlDN_AT1BT10002.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-2/rawdata/12nov29_C129_1_pnlDN_AT1BT10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        12nov29_C129_1_pnlDN_AT1BT10002.rec,  Reconstruction (Pixel Size 1.96 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-2/3dimage_24039/12nov29_C129_1_pnlDN_AT1BT10002.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vhexq-1pn04
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC115
description: <strong>Tilt Series Date:</strong> 2014-09-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 3 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Wait time 10 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-6/rawdata/</p>20140915_YWC115_10006.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-6/rawdata/20140915_YWC115_10006.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140915_YWC115_10006_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-09-15-6/3dimage_46237/20140915_YWC115_10006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/15k3n-6x275
title: Hylemonella gracilis

description: Raw data files of Hylemonella gracilis
description: <strong>Tilt Series Date:</strong> 2012-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 112.5 eV/Å²,  defocus: -15.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2012-09-23-74_slicer6381.jpg: Unknown
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-74/rawdata/</p>20120923_Hylemonella_30004.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-74/rawdata/20120923_Hylemonella_30004.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120923_Hylemonella_30004_full.rec,  Reconstruction (Pixel Size 1.96 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-74/3dimage_20996/20120923_Hylemonella_30004_full.rec"
        > <i class="download icon"></i> Download </a></p>