cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j05h7f40
title: CIL:30566, Drosophila melanogaster, early embryonic cell. In Cell Image Library

description: A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and anti-lamin-B antibodies during prometaphase. The microinjection of a mixture of anti-lamin-B mAbs crosslinks the lamin-B envelope into a hyperstable network that fails to disassemble and remains throughout mitosis. This structure impedes spindle elongation. This image is original data contributing to Fig. 6 "Functional perturbation of the lamin-B envelope interferes with spindle length changes and the completion of mitosis" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.
description: Embryos expressing lamin-B-GFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. For injections, embryos were dehydrated for 3-6 min, covered with halocarbon oil and injected (as described in Brust-Mascher & Scholey, 2009). For double injections, embryos were allowed to recover for 5–20 min between injections. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Green fluorescent proteins from Aequorea; Rhodamine
Data qualification: Raw
DOI: 10.6075/j0f76ckb
title: CIL:30565, Dictyostelium discoideum, Saccharomyces cerevisiae, amoeboid cell, budded yeast. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy; bright-field microscopy
Parameter imaged: fluorescence emission; absorption of illumination
Source of contrast: distribution of a specific protein; differences in intrinsic optical density
Visualization methods: EGFP; mRFP1
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: Severing of budded yeast. Amoebae (D. discoideum) were plated with yeast (S. cerevisiae) in a glass bottom chamber and viewd by confocal microscopy. The amoebae were expressing mRFP-LimEdelta (red) to label actin filaments and PHcrac-GFP (green) to label the membrane of the nascent phagocytic cup, which is rich in phosphatidylinositol (3,4,5) trisphosphate. The phagocytic cup partially engulfs both lobes of a budded yeast; the cup initially extends beyond the mother-bud neck. The cup then retracts back to the neck, and actin filaments strongly accumulate there, applying force. This situation persists for six minutes, ending with severing of the yeast, sealing of the cup, and ingestion of one lobe of the severed yeast. The time series was collected at 3.94-second intervals using a Zeiss LSM 510 laser scanning confocal microscope equipped with a 63x 1.4 NA DIC objective lens. Pixel size 0.12 x 0.12 microns.
DOI: 10.6075/j0x0660k
title: CIL:32023, Xenopus laevis, neurecto-epithelial cell. In Cell Image Library

description: Neural plate of Xenopus laevis at early neural tube closure.
description: Fluorescent fusion proteins encoded by mRNAs, transcribed in vitro, are used to label appropriate portions of cells (i.e., chromosomes via H2B-GFP or membranes). Mixes of mRNA are microinjected with glass needles directly into the embryo at the four-cell stage by use of a picospritzer (see 1 in the ﬁgure). For analysis of the early neural plate, the two dorsal cells are injected. Embryos are then grown to stage 13 for early neural plate development. Embryos were imaged on a Zeiss LSM 510 using a 40X objective.
description: Preparation: whole mounted tissue
Relation to intact cell: living tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of DNA
Visualization methods: EGFP
Processing history: z stack projection
DOI: 10.6075/j0d21w8k
title: CIL:32024, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library

description: Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP comets throughout the cell. This video shows the number of EB3-GFP comets detected after nocodazole perturbation in an RCC-4 cell that is VHL -/- (Von Hippel-Lindau). Compare with experimental video (RCC-4 supplemented with active VHL) in CIL # 26571; there is an increased number of growing MTs when VHL is active. RCC-4 cells were infected with a retroviral vector to express EB3-GFP. Cells on round coverslips were transferred to a homemade holding device, and 600 µl of the appropriate medium, containing 40 nM nocodazole was added. The video was acquired with a microscope (IX70 Delta Vision Spectris; Olympus), temperature-controlled at 37C, using a 60× NA 1.4 differential interference contrast (DIC) oil Plan-Apochromat objective, ex 470 em 520, and a camera (CoolSNAP HQ; Roper Industries) with an exposure time of 100 ms and a frame rate of 0.5 s. Acquisition software used was SoftWoRx version 3.3.4 (Applied Precision). This is the raw image file for the left half of Video 5 in J Cell Biol. 2010. 190: 991-1003.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: widefield illumination; time lapse microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j09022nb
title: CIL:31927, Homo sapiens, retinal pigment epithelium. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.
description: Time lapse movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. The time series of images used to prepare the movie is grouped with it in the Cell Image Library.
DOI: 10.6075/j0rj4hcf
title: CIL:31926, Homo sapiens, retinal pigment epithelium. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. The time series of images used to prepare the movie is grouped with it in the Cell Image Library.
description: Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.
DOI: 10.6075/j0zc82wf
title: CIL:3070, Rattus, multipolar neuron. In Cell Image Library

description: Development of the axon and dendritic arbors in cultured hippocampal neurons after 7 days in vitro. MAP2 staining (red) highlights the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Neurons at 2, 3, 5 and 7 days in vitro are represented in this image group. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 20X (Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Image generated with the MetaMorph color combine function.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
DOI: 10.6075/j0wh2q1k
title: CIL:3066, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
description: Development of the axon and dendritic arbors in cultured hippocampal neurons after 7 days in vitro. MAP2 staining (red) highlights the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Neurons at 2, 3, 5 and 7 days in vitro are represented in this image group. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 20X (Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Image generated with the MetaMorph color combine function.
DOI: 10.6075/j0sf2w68
title: CIL:3062, Rattus, multipolar neuron. In Cell Image Library

description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
description: Development of the axon and dendritic arbors in cultured hippocampal neurons after 7 days in vitro. MAP2 staining (red) highlights the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Neurons at 2, 3, 5 and 7 days in vitro are represented in this image group. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 20X (Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Image generated with the MetaMorph color combine function.
DOI: 10.6075/j0jm28hd
title: CIL:30569, Drosophila melanogaster, early embryonic cell. In Cell Image Library

description: Embryos expressing gfp-tubulin and histone-rfp were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confcal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.
description: A confocal 4D-stack from Cycle 11 in a transgenic Drosophila embryo expressing gfp-tubulin (shown in green) and histone-rfp (shown in red). This image is original data contributing to Fig. S1. "Spindle dynamics in Drosophila embryos and in vitro gliding assays with Ncd and KLP61F" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.
description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Green fluorescent proteins from Aequorea; Rhodamine
Data qualification: Raw
DOI: 10.6075/j05x27j4
title: CIL:31203, Gallus gallus, neuron. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: membrane potential dye accumulation
Visualization methods: CMX rosamine (Mitotracker Red)
Processing history: unprocessed raw data
Data qualification: Raw
description: Mitochondrial transport during a growth cone pause. Chicken DRG neurons were grown on coverslips coated with laminin/poly-L-ornithine for 2 d and stained with 0.1 µM MitoTracker Red CMXRos. Images were acquired every 2 s. To reduce the size of the move, one frame for every 8 s is shown. In the movie, the growth cone is toward the right-hand side. The time elapsed is indicated in the top left hand corner. During the first 10 min of the movie, the growth cone advances. It then pauses, but low velocity anterograde transport of the docked mitochondria continues unabated. This movie illustrates low velocity transport continues during prolonged growth cone pauses. Bar, 10 µm.

Images were acquired on a confocal system (FV300 FluoView; Olympus) on an IX70 microsocpe using a 60×, 1.4 NA, PlanApo objective. Samples were illuminated with an argon/krypton Omnichrome 488/568/647 line laser set to standby mode, with the laser intensity set at 6%, the confocal aperture set at 5, and pixel dwell times of either 100 or 200 microsec; the light was further attenuated with neutral density filters (either 25 or 50%). Cells were observed in an enclosed flow chamber. Temperature was maintained at 37°C. Movie corresponds to Video 3 in J Cell Biol. 2006 May 8;173(3):373-81.
DOI: 10.15468/dl.ad4g8m
title: Occurrence Download

description: A dataset containing 296 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Nepal",
 "OccurrenceStatus is Present",
 "TaxonKey is Panthera tigris"
 ]
}

 The dataset includes 296 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009410-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j0j101vc
title: CIL:31202, Gallus gallus, neuron. In Cell Image Library

description: Mitochondrial transport during axonal elongation. Chicken DRG neurons were grown on coverslips coated with laminin/poly-L-ornithine for 2 d and stained with 0.1 µM MitoTracker red CMXRos. Images were acquired every 2 s, but to reduce the size of the movie, one frame for every 4 s is shown. In the movie, the growth cone is toward the right-hand side. During the process of growth cone advance, mitochondria docked along the axon move at a low velocity in the anterograde direction in a coherent manner. During periods of rapid growth cone advance, mitochondria in the growth cone are left behind in regions of newly consolidated axon (between the 30- and 45-min time points). During periods of growth cone pauses, the low velocity anterograde transport of docked mitochondria continues (between the 52- and 62-min time periods). Over the course of the movie, increases in the distance between pairs of docked mitochondria can be observed. This movie illustrates low velocity transport during axonal elongation and a brief pause. Bar, 10 µm.

 
Images were acquired on a confocal system (FV300 FluoView; Olympus) on an IX70 microsocpe using a 60×, 1.4 NA, PlanApo objective. Samples were illuminated with an argon/krypton Omnichrome 488/568/647 line laser set to standby mode, with the laser intensity set at 6%, the confocal aperture set at 5, and pixel dwell times of either 100 or 200 microsec; the light was further attenuated with neutral density filters (either 25 or 50%). Cells were observed in an enclosed flow chamber. Temperature was maintained at 37°C. This movie corresponds to video 2 from J Cell Biol. 2006 May 8;173(3):373-81.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: membrane potential dye accumulation
Visualization methods: CMX rosamine (Mitotracker Red)
Processing history: unprocessed raw data
Data qualification: Raw
DOI: 10.6075/j01n7zsv
title: CIL:31201, Gallus gallus, neuron. In Cell Image Library

description: Chicken DRG neurons were grown on coverslips coated with laminin/poly-L-ornithine for 2 d and stained with 0.1 µM MitoTracker red CMXRos. Images were acquired every 2 s. To reduce the size of the video, one frame for every 10 s is shown. In the video, both DIC and fluorescent images are shown. The growth cone is toward the right-hand side. The time elapsed is indicated in the top left hand corner. During the process of growth cone advance, docked mitochondria moved at a low velocity in the anterograde direction in a coherent manner. A kymograph of the movie is shown at the bottom of the screen. Time is on the y axis and distance is on the x axis. The moving bar on the right hand side of the kymograph marks the time position. This video illustrates how kymographs correspond to time-lapse movies of mitochondrial transport, Bar, 10 µm. 

Images were acquired on a confocal system (FV300 FluoView; Olympus) on an IX70 microsocpe using a 60×, 1.4 NA, PlanApo objective. Samples were illuminated with an argon/krypton Omnichrome 488/568/647 line laser set to standby mode, with the laser intensity set at 6%, the confocal aperture set at 5, and pixel dwell times of either 100 or 200 microsec; the light was further attenuated with neutral density filters (either 25 or 50%). Cells were observed in an enclosed flow chamber maintained at 37°C. This video corresponds to Figure 1 and video 1 from J Cell Biol. 2006 May 8;173(3):373-81.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image; kymograph
Imaging mode: differential interference contrast microscopy; single-spot confocal microscopy
Parameter imaged: optical path length gradient; fluorescence emission
Source of contrast: boundaries between regions with different refractive index; membrane potential dye accumulation
Visualization methods: visualization of contiguous regions; CMX rosamine (Mitotracker Red)
Processing history: unprocessed raw data; line profile
Data qualification: Raw
DOI: 10.6075/j0m32vr1
title: CIL:3074, Rattus, multipolar neuron. In Cell Image Library

description: Development of the axon and dendritic arbors in cultured hippocampal neurons after 7 days in vitro. MAP2 staining (red) highlights the dendrites, while microtubule staining (green) reveals both the axons and dendrites in the field of view. Neurons at 2, 3, 5 and 7 days in vitro are represented in this image group. 

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for tubulin (monoclonal DM1A, from Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]) and MAP2 (polyclonal Ab266, from S. Halpain with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 20X (Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. Image generated with the MetaMorph color combine function.
description: Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data