DOI: 10.5061/dryad.m905qfv2d
title: Seed sizes for mixed pollen and pure-species pollinations

description: Hybrid Seed Inviability (HSI) is a common barrier in angiosperms. Recent
 work suggests that the rapid evolution of HSI may, in part, be due to
 conflict between maternal and paternal optima for resource allocation to
 developing offspring (i.e. parental conflict). However, parental conflict
 requires that paternally-derived resource acquiring alleles impose a
 maternal cost. I test this requirement using three closely related species
 in the Mimulus guttatus species complex that exhibit significant HSI and
 differ in their inferred histories of parental conflict. I show that the
 presence of hybrid seeds significantly affects conspecific seed size for
 almost all crosses, such that conspecific seeds are smaller after
 developing with hybrids from fathers with a stronger history of conflict,
 and larger after developing with hybrids from fathers with a weaker
 history of conflict. This work demonstrates a cost of paternally-derived
 alleles and also has implications for species fitness in secondary
 contact.
description: Pollinations were completed for three species of
 <em>Mimulus</em> for a total of 9 different cross types:
 purely self-fertilized and mixed pollinations involving a roughly equal
 amount of self pollen and pollen from another species. The resultant
 fruits were then counted and quantified for: the number of seeds produced
 and their viability. Based on diagnostic seed characteristics, seeds were
 categorized as hybrid or conspecific. All conspecific seeds were measured
 for total area using ImageJ.
description: # Seed sizes for mixed pollen and pure-species pollinations
 [https://doi.org/10.5061/dryad.m905qfv2d](https://doi.org/10.5061/dryad.m905qfv2d) ## Description of the data and file structure '**Areas_Coughlan. 2021. ForDryad.txt**' contains all data pertaining to seed size measurements for each fruit, including data corresponding to the maternal and paternal donor(s) of that fruit. Column titles: * Mat= maternal genotype, * mat.rep= replicate plant of that genotype, * Pat= paternal genotype(s), * cross.rep= replicate fruit for that maternal/paternal combination, * viable= number of viable seeds produced, * inviable= number of inviable seeds produced, * total= total seeds produced, * proportion= proportion of seeds that are enviable, * mixed or self= status of the pollen bouquet; containing self pollen and pollen from another species (mixed) or just self pollen (self), * particle.number= seed numerical ID per fruit, * area.mm= individual seed size (in mm). ‘**PerFruitMeasurements.csv**’ contains all data pertaining to the fruits collected. These data are similar to the “Areas_Coughlan.2021.ForDryad.csv’ file, except contain the average conspecific seed size per fruit (AA) and the data pertaining to deviations of an expected 1:1 paternal genotype ratio. * Mat= maternal genotype, * MR= replicate plant of that genotype, * Pat= paternal genotype(s), * CR= replicate fruit for that maternal/paternal combination, * viable= number of viable seeds produced, * inviable= number of inviable seeds produced, * total= total seeds produced, * proportion= proportion of seeds that are enviable, * AA= average conspecific seed area per fruit (in mm), * Sig?=  is there a significant deviation from 1:1 pollination of the two parental genotypes (SIG=significant deviation, NS= not significant, NA= not applicable (i.e. only one paternal donor)). **'Coughlan.2022.AmNat.Rmd'** Code for analyzing seed size data.  ## Access information Other publicly accessible locations of the data: * NA Data was derived from the following sources: * photos of seeds analyzed in imageJ to estimate size