cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.3405616
title: Measuring thermal behavior in smaller insects: A case study in <i>Drosophila melanogaster</i> demonstrates effects of sex, geographic origin, and rearing temperature on adult behavior

description: Measuring thermal behavior in smaller insects is particularly challenging. In this study, we describe a new horizontal thermal gradient apparatus designed to study adult thermal behavior in small insects and apply it using <i>D. melanogaster</i> as a model and case study. Specifically, we used this apparatus and associated methodology to examine the effects of sex, geographic origin, and developmental rearing temperature on temperature preferences exhibited by adults in a controlled laboratory environment. The thermal gradient established by the apparatus was stable over diurnal and calendar time. Furthermore, the distribution of adult flies across thermal habitats within the apparatus remained stable following the period of acclimation, as evidenced by the high degree of repeatability across both biological and technical replicates. Our data demonstrate significant and predictable variation in temperature preference for all 3 assayed variables. Behaviorally, females were more sensitive than males to higher temperatures. Flies originating from high latitude, temperate populations exhibited a greater preference for cooler temperatures; conversely, flies originating from low latitude, tropical habitats demonstrated a relative preference for higher temperatures. Similarly, larval rearing temperature was positively associated with adult thermal behavior: low culture temperatures increased the relative adult preference for cooler temperatures, and this response was distinct between the sexes and for flies from the temperate and subtropical geographic regions. Together, these results demonstrate that the temperature chamber apparatus elicits robust, predictable, and quantifiable thermal preference behavior that could readily be applied to other taxa to examine the role of temperature-mediated behavior in a variety of contexts.
DOI: 10.6084/m9.figshare.3405616.v2
title: Measuring thermal behavior in smaller insects: A case study in <i>Drosophila melanogaster</i> demonstrates effects of sex, geographic origin, and rearing temperature on adult behavior

description: Measuring thermal behavior in smaller insects is particularly challenging. In this study, we describe a new horizontal thermal gradient apparatus designed to study adult thermal behavior in small insects and apply it using <i>D. melanogaster</i> as a model and case study. Specifically, we used this apparatus and associated methodology to examine the effects of sex, geographic origin, and developmental rearing temperature on temperature preferences exhibited by adults in a controlled laboratory environment. The thermal gradient established by the apparatus was stable over diurnal and calendar time. Furthermore, the distribution of adult flies across thermal habitats within the apparatus remained stable following the period of acclimation, as evidenced by the high degree of repeatability across both biological and technical replicates. Our data demonstrate significant and predictable variation in temperature preference for all 3 assayed variables. Behaviorally, females were more sensitive than males to higher temperatures. Flies originating from high latitude, temperate populations exhibited a greater preference for cooler temperatures; conversely, flies originating from low latitude, tropical habitats demonstrated a relative preference for higher temperatures. Similarly, larval rearing temperature was positively associated with adult thermal behavior: low culture temperatures increased the relative adult preference for cooler temperatures, and this response was distinct between the sexes and for flies from the temperate and subtropical geographic regions. Together, these results demonstrate that the temperature chamber apparatus elicits robust, predictable, and quantifiable thermal preference behavior that could readily be applied to other taxa to examine the role of temperature-mediated behavior in a variety of contexts.
DOI: 10.6084/m9.figshare.3405616.v1
title: Measuring thermal behavior in smaller insects: a case study in <i>Drosophila melanogaster</i> demonstrates effects of sex, geographic origin, and rearing temperature on adult behavior

description: Measuring thermal behavior in smaller insects is particularly challenging. In this study, we describe a new horizontal thermal gradient apparatus designed to study adult thermal behavior in small insects and apply it using <i>D. melanogaster</i> as a model and case study. Specifically, we used this apparatus and associated methodology to examine the effects of sex, geographic origin, and developmental rearing temperature on temperature preferences exhibited by adults in a controlled laboratory environment. The thermal gradient established by the apparatus was stable over diurnal and calendar time. Furthermore, the distribution of adult flies across thermal habitats within the apparatus remained stable following the period of acclimation, as evidenced by the high degree of repeatability across both biological and technical replicates. Our data demonstrate significant and predictable variation in temperature preference for all three assayed variables. Behaviorally, females were more sensitive than males to higher temperatures. Flies originating from high latitude, temperate populations exhibited a greater preference for cooler temperatures; conversely, flies originating from low latitude, tropical habitats demonstrated a relative preference for higher temperatures. Similarly, larval rearing temperature was positively associated with adult thermal behavior: low culture temperatures increased the relative adult preference for cooler temperatures, and this response was distinct between the sexes and for flies from the temperate and subtropical geographic regions. Together, these results demonstrate that the temperature chamber apparatus elicits robust, predictable, and quantifiable thermal preference behavior that could readily be applied to other taxa to examine the role of temperature-mediated behavior in a variety of contexts.
DOI: 10.6084/m9.figshare.3405610
title: Regulation of monocyte induced cell migration by the RNA binding protein, FXR1

description: FXR1 belongs to a family of RNA-binding proteins that play critical roles in post-transcriptional regulation of gene expression in immunity, development and cancer. FXR1 is associated with regulation of specific mRNAs in myocytes and macrophages. In quiescent cells (&gt; 24 h of extended serum-starvation, ∼30-48 h or more), a spliced isoform of FXR1, FXR1a, promotes translation of the cytokine TNFα, independent of the effects of RNA levels. Here we examined the role of FXR1 in THP1 human monocytic leukemic cells that were grown in serum, as well as in early (24 h) serum-starvation conditions that demonstrates differences in gene expression mechanisms and is distinct from quiescent (&gt; 24 h extended serum-starvation) cells. Global RNA profiling, conducted to investigate the role of FXR1 on mRNA levels, revealed that FXR1 affects levels of specific mRNAs in serum-grown and in early 24 h serum-starvation conditions. FXR1 decreases levels of several mRNAs, including as previously identified, CDKN1A (p21CIP1 or p21) mRNA in serum-grown cells. Interestingly, we find that FXR1 positively regulates mRNA levels of specific cytokines and chemokines in serum-grown and in early 24 h serum-starvation conditions. These include IL1β and CCL2 that control cell migration. Accordingly, depletion and overexpression of FXR1 decreased and increased levels of CCL2 mRNA. Consistent with the reduced levels of IL1β, CCL2 and other chemokines upon FXR1 depletion, our data reveal that depletion of FXR1 decreases the ability of these cells to induce cell migration of neighboring monocytic cells. These data reveal a new role of FXR1 in controlling induction of monocyte migration.
DOI: 10.6084/m9.figshare.3405577
title: Design and Evaluation of Illumina MiSeq Compatible 18S rRNA Gene Primers for Improved Characterization of Mixed Phototrophic Communities

description: A systematic analysis of the most commonly amplified 18S rRNA hypervariable regions and the effect that PCR/sequencing bias has on community representation. The V4 and V8-V9 hypervariable regions were amplified for seven mock communities and for freshwater, marine, and wastewater samples to compare the sequenced community structure to theoretical communities and validate these results with environmental samples. This project contains mothur script files for analyzing the data.
DOI: 10.6084/m9.figshare.3405577.v1
title: Design and Evaluation of Illumina MiSeq Compatible 18S rRNA Gene Primers for Improved Characterization of Mixed Phototrophic Communities

description: A systematic analysis of the most commonly amplified 18S rRNA hypervariable regions and the effect that PCR/sequencing bias has on community representation. The V4 and V8-V9 hypervariable regions were amplified for seven mock communities and for freshwater, marine, and wastewater samples to compare the sequenced community structure to theoretical communities and validate these results with environmental samples. This project contains mothur script files for analyzing the data.
DOI: 10.6084/m9.figshare.3405574
title: Chinese Webinar 2016 - "Get the best out of your papers!"

description: Chinese webinar on: "Get the best out of your papers!" presented by Albert Chan, Market Development Director - China, Dove Medical Press<br>The purpose of this webinar is to help Chinese authors on how to get the best out of their papers through:<br>Manuscript preparationSubmissionPublicationPromotion<br>德孚医药出版社举办的在线研讨会主题：怎样充分展示你论文的优势！ 作者可以采用这4個途径去充分展示论文的优势： 准备提交发表推广<br>研讨会主持人：<br>陈炜山 Albert Chan中国市场拓展总监德孚医药出版社 Dove Medical Press
DOI: 10.6084/m9.figshare.3405574.v2
title: Chinese Webinar 2016 - "Get the best out of your papers!"

description: Chinese webinar on: "Get the best out of your papers!" presented by Albert Chan, Market Development Director - China, Dove Medical Press<br>The purpose of this webinar is to help Chinese authors on how to get the best out of their papers through:<br>Manuscript preparationSubmissionPublicationPromotion<br>德孚医药出版社举办的在线研讨会主题：怎样充分展示你论文的优势！ 作者可以采用这4個途径去充分展示论文的优势： 准备提交发表推广<br>研讨会主持人：<br>陈炜山 Albert Chan中国市场拓展总监德孚医药出版社 Dove Medical Press
DOI: 10.6084/m9.figshare.3405574.v1
title: Chinese Webinar 2016

description: Chinese webinar on: "Get the best out of your papers!" presented by Albert Chan, Market Development Director - China, Dove Medical Press<br>The purpose of this webinar is to help Chinese authors on how to get the best out of their papers through:<br>Manuscript preparationSubmissionPublicationPromotion<br>德孚医药出版社举办的在线研讨会主题：怎样充分展示你论文的优势！ 作者可以采用这4個途径去充分展示论文的优势： 准备提交发表推广<br>研讨会主持人：<br>陈炜山 Albert Chan中国市场拓展总监德孚医药出版社 Dove Medical Press
DOI: 10.6084/m9.figshare.3405232
title: Molecular mechanism of the susceptibility difference between HLA-B*27:02/04/05 and HLA-B*27:06/09 to ankylosing spondylitis: substitution analysis, MD simulation, QSAR modelling, and <i>in vitro</i> assay

description: The human leukocyte antigen HLA-B27 is directly involved in the disease pathogenesis of ankylosing spondylitis (AS). HLA-B27 has a high degree of genetic polymorphism, with 105 currently known subtypes; the presence of aspartic acid at residue 116 (Asp116) has been found to play an essential role in AS susceptibility. Here, we systematically investigated the molecular mechanism of the susceptibility difference between the AS-associated subtypes HLA-B*27:02/04/05 and AS-unassociated subtypes HLA-B*27:06/09 to AS at sequence, structure, energetic and dynamic levels. In total seven variable residues were identified among the five studied HLA-B27 subtypes, in which Asp116 can be largely stabilized by a spatially vicinal, positively charged His114 through a salt bridge, while five other variable residues seem to have only a marginal effect on AS susceptibility. We also employed a quantitative structure–activity relationship approach to model the statistical correlation between peptide structure and affinity to HLA-B*27:05, a genetic ancestor of all other HLA-B27 subtypes and associated strongly with AS. The built regression predictor was verified rigorously through both internal cross-validation and external blind validation, and was then employed to identify potential HLA-B*27:05 binders from &gt;20,000 cartilage-derived self-peptides. Subsequently, the binding potency of the top five antigenic peptides to HLA-B*27:05 was assayed <i>in vitro</i> using a FACS-based MHC stabilization experiment. Consequently, two (QRVGSDEFK and LRGAGTNEK) out of the five peptides were determined to have high affinity (BL<sub>50</sub> = 5.5 and 15.8 nM, respectively) and, as expected, both of them possess positively charged Lys at the C-terminus.
DOI: 10.6084/m9.figshare.3405232.v1
title: Molecular mechanism of the susceptibility difference between HLA-B*27:02/04/05 and HLA-B*27:06/09 to ankylosing spondylitis: substitution analysis, MD simulation, QSAR modelling, and <i>in vitro</i> assay

description: The human leukocyte antigen HLA-B27 is directly involved in the disease pathogenesis of ankylosing spondylitis (AS). HLA-B27 has a high degree of genetic polymorphism, with 105 currently known subtypes; the presence of aspartic acid at residue 116 (Asp116) has been found to play an essential role in AS susceptibility. Here, we systematically investigated the molecular mechanism of the susceptibility difference between the AS-associated subtypes HLA-B*27:02/04/05 and AS-unassociated subtypes HLA-B*27:06/09 to AS at sequence, structure, energetic and dynamic levels. In total seven variable residues were identified among the five studied HLA-B27 subtypes, in which Asp116 can be largely stabilized by a spatially vicinal, positively charged His114 through a salt bridge, while five other variable residues seem to have only a marginal effect on AS susceptibility. We also employed a quantitative structure–activity relationship approach to model the statistical correlation between peptide structure and affinity to HLA-B*27:05, a genetic ancestor of all other HLA-B27 subtypes and associated strongly with AS. The built regression predictor was verified rigorously through both internal cross-validation and external blind validation, and was then employed to identify potential HLA-B*27:05 binders from &gt;20,000 cartilage-derived self-peptides. Subsequently, the binding potency of the top five antigenic peptides to HLA-B*27:05 was assayed <i>in vitro</i> using a FACS-based MHC stabilization experiment. Consequently, two (QRVGSDEFK and LRGAGTNEK) out of the five peptides were determined to have high affinity (BL<sub>50</sub> = 5.5 and 15.8 nM, respectively) and, as expected, both of them possess positively charged Lys at the C-terminus.
DOI: 10.6084/m9.figshare.3404728
title: Regression Data and Results for The Impact of Services on Economic Complexity

description: This file contains the data and results from the regression analysis performed in the paper: The Impact of Services on Economic Complexity: Service Sophistication as Route for Economic Growth by Stojkoski V, Utkovski Z, Kocarev L<br>
DOI: 10.6084/m9.figshare.3404677
title: Potential signatures of heavy metal complexes in lichen reflectance spectra

description: Lichens are sensitive to atmospheric pollutants emitted from anthropogenic activities and are thus effective biomonitors. A variety of heavy metals, such as nickel (Ni), iron (Fe), lead (Pb), copper (Cu), and cadmium (Cd), can be emitted by metal smelters. The purpose of this study was twofold: (1) to measure the spectral reflectance properties (350–2500 nm) of expected heavy metal complexes in lichens (oxalates and sulphides); and (2) to determine whether these complexes contribute features to reflectance spectra of lichens from the vicinity of a heavy metal smelter. Some metal oxalate spectra are characterized by crystal field transition absorption bands in the 500–1300 nm region, which are specific to the particular metal cation they contain and its oxidation state. The 1900–2500 nm region exhibits multiple absorption bands attributable to the oxalate molecule. The metal sulphide reflectance spectra are characterized by generally low reflectance and few if any strong or diagnostic spectral features; those that are found can be related to a specific cation and its oxidation state. These spectra were used to determine whether reflectance spectra of a diverse suite of lichens collected downwind of a smelter showed spectral evidence indicative of heavy metal oxalates or sulphides. The lichen spectra, coupled with the oxalate and sulphide spectra and independently determined heavy metal concentration, failed to reveal spectral features that could be unambiguously related to heavy metal complexes. This was likely due to a number of causes: lichen reflectance spectra have absorption bands that overlap those of oxalates; oxalate and sulphide concentrations may have been too low to allow for their unambiguous identification, and lichen spectra are naturally diverse in the region below 1300 nm. There were no strong or significant linear trends between metal concentrations and distance from the smelter (coefficient of determination (<i>R</i><sup>2</sup>) values &lt;0.05), or between absorption band depths in the lichen spectra and distance from the smelter (<i>R</i><sup>2</sup> values &lt;0.06). This was likely due to the inclusion of multiple lichen species in the analysis, which may interact with airborne pollutants in different ways, and microenvironmental effects.
DOI: 10.6084/m9.figshare.3404677.v1
title: Potential signatures of heavy metal complexes in lichen reflectance spectra

description: Lichens are sensitive to atmospheric pollutants emitted from anthropogenic activities and are thus effective biomonitors. A variety of heavy metals, such as nickel (Ni), iron (Fe), lead (Pb), copper (Cu), and cadmium (Cd), can be emitted by metal smelters. The purpose of this study was twofold: (1) to measure the spectral reflectance properties (350–2500 nm) of expected heavy metal complexes in lichens (oxalates and sulphides); and (2) to determine whether these complexes contribute features to reflectance spectra of lichens from the vicinity of a heavy metal smelter. Some metal oxalate spectra are characterized by crystal field transition absorption bands in the 500–1300 nm region, which are specific to the particular metal cation they contain and its oxidation state. The 1900–2500 nm region exhibits multiple absorption bands attributable to the oxalate molecule. The metal sulphide reflectance spectra are characterized by generally low reflectance and few if any strong or diagnostic spectral features; those that are found can be related to a specific cation and its oxidation state. These spectra were used to determine whether reflectance spectra of a diverse suite of lichens collected downwind of a smelter showed spectral evidence indicative of heavy metal oxalates or sulphides. The lichen spectra, coupled with the oxalate and sulphide spectra and independently determined heavy metal concentration, failed to reveal spectral features that could be unambiguously related to heavy metal complexes. This was likely due to a number of causes: lichen reflectance spectra have absorption bands that overlap those of oxalates; oxalate and sulphide concentrations may have been too low to allow for their unambiguous identification, and lichen spectra are naturally diverse in the region below 1300 nm. There were no strong or significant linear trends between metal concentrations and distance from the smelter (coefficient of determination (<i>R</i><sup>2</sup>) values &lt;0.05), or between absorption band depths in the lichen spectra and distance from the smelter (<i>R</i><sup>2</sup> values &lt;0.06). This was likely due to the inclusion of multiple lichen species in the analysis, which may interact with airborne pollutants in different ways, and microenvironmental effects.
DOI: 10.6084/m9.figshare.3404668
title: The Influences on Teaching Perspectives of Australian Physical Education Teacher Education Students: The First-Year Influences on Teaching Perspectives Exploratory (FIT-PE) Study

description: <em>There has been a paucity of literature investigating the teaching beliefs and intentions of Australian physical education teacher education (PETE) students that enter teacher training. The First-year Influences on Teaching Perspectives Exploratory (FIT-PE) study explores the teaching perspectives of first year PETE students; including teaching perspectives predicted as being dominant and important for physical education teaching. The teaching perspectives inventory (TPI) was administered to 105 Australian PETE students. Independent t-tests and one-way ANOVA statistical tests were conducted to compare average teaching perspective summary scores across demographic variables. The FIT-PE study findings revealed 18 year olds (compared to 20-25 year olds) and PETE students from rural backgrounds (compared to regional) had significantly higher average summary scores for the transmission (content-oriented) teaching perspective. This paper provides reflective opportunities for teacher training programs of the underlying core teaching values (beliefs and intentions) of students at the entry point of PETE training.</em>
DOI: 10.6084/m9.figshare.3404668.v1
title: The Influences on Teaching Perspectives of Australian Physical Education Teacher Education Students: The First-Year Influences on Teaching Perspectives Exploratory (FIT-PE) Study

description: <em>There has been a paucity of literature investigating the teaching beliefs and intentions of Australian physical education teacher education (PETE) students that enter teacher training. The First-year Influences on Teaching Perspectives Exploratory (FIT-PE) study explores the teaching perspectives of first year PETE students; including teaching perspectives predicted as being dominant and important for physical education teaching. The teaching perspectives inventory (TPI) was administered to 105 Australian PETE students. Independent t-tests and one-way ANOVA statistical tests were conducted to compare average teaching perspective summary scores across demographic variables. The FIT-PE study findings revealed 18 year olds (compared to 20-25 year olds) and PETE students from rural backgrounds (compared to regional) had significantly higher average summary scores for the transmission (content-oriented) teaching perspective. This paper provides reflective opportunities for teacher training programs of the underlying core teaching values (beliefs and intentions) of students at the entry point of PETE training.</em>
DOI: 10.6084/m9.figshare.3404662
title: Partner Choice in Raven (Corvus corax) Cooperation

description: Data of:Asakawa-Haas, K., Schiestl, M., Bugnyar, T & Massen, J.J.M. (2016). Partner choice in raven (Corvus corax) cooperation. PLoS ONE
DOI: 10.6084/m9.figshare.3404647
title: dental CBCT data 2

description: resolution: 512*512*244； unsigned short； a 0.3mm resolution.This dataset was obtained using the dental CBCT imaging system ZCB100 (Shenzhen ZhongKe TianYue Technology Co., Ltd.) at 110 kV and 10 mAs, with a 15.36-cm FOV