DOI: 10.5061/dryad.fttdz091j
title: Data From: Dietary restriction extends lifespan across different temperatures in the fly

description: Dietary restriction (DR) has been consistently shown to extend lifespan
 across a range of taxa. However, recent findings question the universality
 of the DR longevity response, suggesting DR may not extend lifespan at
 lower temperatures in flies, and that the DR longevity response is an
 artifact of benign laboratory conditions.  We re-test this
 hypothesis, measuring the lifespan of Drosophila melanogaster at the
 lab-standard 25°C, and at colder temperatures (18°C and 21°C) across a
 range of 5 diets.  We found the DR longevity response to be
 robust, extending lifespan irrespective of temperature. Fecundity was
 measured as a positive control for the DR phenotype, and it was found that
 DR reduced egg laying. We suggest results that question DR as a phenotype
 should not be overinterpreted readily, as variations in the experimental
 setup, genetic lines used, and diet-lifespan reaction norm may be
 responsible for discrepancies. Additionally, starting conditions that show
 a lifespan extension by DR and then changing the environment and/or
 genotype promises a more robust test of DR modulating factors.
description: # Data From: Dietary restriction extends lifespan across different
 temperatures in the fly We have submitted our raw data for our survival
 curves showing the longevity response of yw flies to dietary restriction
 at standard lab temperatures. (Lifespan_25°C_ywRflies.csv), cold
 temperatures (Lifespan_18°Cand21°C_ywRflies.csv), and their fecundity at
 cold temperatures (EggCounting_18°Cand21°C_ywRflies.csv). Data is
 presented in an accepted manuscript for Functional Ecology. ##
 Descriptions **Lifespan Data** The following details are the same across
 both lifespan datasets: * Individual: Cox proportional hazard model
 requires that each row in the dataset represents a single individual. *
 Genotype: The code M22 is used by the lab to refer to a wild-type strain
 of Drosophila melanogaster known as ywR flies. * Cage: Number assigned to
 the demography cage each fly was kept in. This was recorded so Cage could
 then be included as a random term in our Cox proportional hazard model. *
 Age: The age at which each individual died (Days) * At.Risk: Number of
 flies still alive in the demography cage at the point of the
 individual's death. This is included to be able to plot mortality
 risk if so required. * Transfer: The age-matched cohort that each
 individual belonged to. * Dead: A binary value of 1 or 0 represents
 whether or not the hazard (death) has occurred. Lifespan experiments
 concluded when all flies were dead. The following details are specific to
 the named dataset: **Lifespan_25°C_ywRflies** * Treatment: The yeast
 content of fly diets (%). * Experiment: Refers to the experiments included
 the survival curve shown in Fig.1. Each experiment is identified by the
 initials of the researcher who led the experiment and the corresponding
 experiment letter used in the survival curve. *
 Lifespan_18°Cand21°C_ywRflies * Treatment: Temperature condition for each
 individual (21 or 18 (°C)) followed by the yeast content of the fly diet
 (2,4,6,8 or 10 (%)). * Egg Counting Data
 **EggCounting_18°Cand21°C_ywRflies** * Vial Date: Date when vials,
 containing food on which eggs were laid, were removed from demography
 cages. Vials were collected on two dates, considered to be roughly a
 midpoint in the lifespan of the fly populations at the highest yeast
 diets. * Date counted: Date of manual counts of eggs laid in each vial.
 Eggs were stored at 4°C after collection and until they were counted to
 prevent eggs from hatching and therefore avoid miss-counts. * Vial Number:
 Corresponds to the demography cages each vial was taken from. * Temp:
 Temperature of each corresponding demography cage was kept at (°C). *
 Conc: The yeast content of fly diets (%). * Egg Count: Number of eggs per
 counted in each vial, including eggs on vial base and eggs on residual
 food on sides of vial. * Age: Age of cohort of flies per demography cage
 at the time of vial collection, calculated using date flies eclosed (DOB).
 * Females per Cage: Number of females per corresponding demography cage,
 used with Egg Count to calculate the number of Eggs per Female.