cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11440431
title: Genetic and morphological diversity and population structure of a polyploid complex of <i>Mimosa</i> (Leguminosae)

description: Evolutionary processes in plants such as hybridization and polyploidy are relevant to speciation and little studied in subtropical South America. In subtropical South America, the genus <i>Mimosa</i> is highly diverse, comprising 30% of polyploid species. The origin and genetic and morphological structure of polyploids in <i>Mimosa</i> are only very recently investigated, as well as their relevance to speciation, morphological variation, and distribution. <i>Mimosa</i> subser. <i>Dolentes–Brevipedes</i>, a taxonomic complex from that region, exhibits polyploidy, with specific and infraspecific taxa confusedly circumscribed. Studies of its population biology would facilitate the elucidation of evolutionary processes in <i>Mimosa</i> and resolution of taxonomic conflicts. We performed a multidisciplinary study of this complex, analysing seven populations and 87 individuals by means of morphometry, and AFLP fingerprinting throughout its area of distribution. Five populations included several taxa and intermediate individuals. Morphometric analyses revealed three clusters, distinguished mainly by leaf morphology and inflorescence. One population was tetraploid, one was tetraploid/octoploid and the rest octoploid. Genetic differentiation was high (<i>Phi<sub>PT</sub></i> = 0.277) and expected heterozygosity was moderate (<i>He =</i> 0.190; <i>H =</i> 0.271). Multivariate molecular analyses revealed genetic divergence between highland and lowland grasslands. Structural genetic analyses revealed three clusters: two mixed, distinctly predominant in lowland/highland grasslands; another, exclusively from Mercedes. Molecular analysis of variance showed significant differences between highland/lowland grasslands and cytotypes. Cytotypes and populations differed partially by morphology. Tetraploids were morphologically similar to the sympatric octoploids, showing similar genetic structure and gene flow; furthermore, tetraploids are involved in origin of these higher polyploids. The systematics of this complex requires revision: subseries <i>Dolentes</i> and some <i>Brevipedes</i> species could be considered as one subseries. Admixture of genetic clusters and intermediates can be explained by hybridization and introgression. A marginal population differed from the others by morphology and genetics thus suggesting incipient speciation at the geographic edges of this complex.
DOI: 10.6084/m9.figshare.11440431.v1
title: Genetic and morphological diversity and population structure of a polyploid complex of <i>Mimosa</i> (Leguminosae)

description: Evolutionary processes in plants such as hybridization and polyploidy are relevant to speciation and little studied in subtropical South America. In subtropical South America, the genus <i>Mimosa</i> is highly diverse, comprising 30% of polyploid species. The origin and genetic and morphological structure of polyploids in <i>Mimosa</i> are only very recently investigated, as well as their relevance to speciation, morphological variation, and distribution. <i>Mimosa</i> subser. <i>Dolentes–Brevipedes</i>, a taxonomic complex from that region, exhibits polyploidy, with specific and infraspecific taxa confusedly circumscribed. Studies of its population biology would facilitate the elucidation of evolutionary processes in <i>Mimosa</i> and resolution of taxonomic conflicts. We performed a multidisciplinary study of this complex, analysing seven populations and 87 individuals by means of morphometry, and AFLP fingerprinting throughout its area of distribution. Five populations included several taxa and intermediate individuals. Morphometric analyses revealed three clusters, distinguished mainly by leaf morphology and inflorescence. One population was tetraploid, one was tetraploid/octoploid and the rest octoploid. Genetic differentiation was high (<i>Phi<sub>PT</sub></i> = 0.277) and expected heterozygosity was moderate (<i>He =</i> 0.190; <i>H =</i> 0.271). Multivariate molecular analyses revealed genetic divergence between highland and lowland grasslands. Structural genetic analyses revealed three clusters: two mixed, distinctly predominant in lowland/highland grasslands; another, exclusively from Mercedes. Molecular analysis of variance showed significant differences between highland/lowland grasslands and cytotypes. Cytotypes and populations differed partially by morphology. Tetraploids were morphologically similar to the sympatric octoploids, showing similar genetic structure and gene flow; furthermore, tetraploids are involved in origin of these higher polyploids. The systematics of this complex requires revision: subseries <i>Dolentes</i> and some <i>Brevipedes</i> species could be considered as one subseries. Admixture of genetic clusters and intermediates can be explained by hybridization and introgression. A marginal population differed from the others by morphology and genetics thus suggesting incipient speciation at the geographic edges of this complex.
DOI: 10.6084/m9.figshare.11440461.v1
title: Metadata record for: Land use and cover maps for Mato Grosso State in Brazil from 2001 to 2017

description: This dataset contains key characteristics about the data described in the Data Descriptor Land use and cover maps for Mato Grosso State in Brazil from 2001 to 2017. <br> Contents: <br> 1. human readable metadata summary table in CSV format 2. machine readable metadata file in JSON format
DOI: 10.6084/m9.figshare.11440461.v2
title: Metadata record for: Land use and cover maps for Mato Grosso State in Brazil from 2001 to 2017

description: This dataset contains key characteristics about the data described in the Data Descriptor Land use and cover maps for Mato Grosso State in Brazil from 2001 to 2017. <br> Contents: <br> 1. human readable metadata summary table in CSV format 2. machine readable metadata file in JSON format <br> <br> Versioning Note:Version 2 was generated when the metadata format was updated from JSON to JSON-LD. This was an automatic process that changed only the format, not the contents, of the metadata.<br>
DOI: 10.6084/m9.figshare.11440404
title: Marine diatom assemblages of the Nosy Be Island coasts, NW Madagascar: species composition and biodiversity using molecular and morphological taxonomy

description: Madagascar is an isolated oceanic island characterized by high degrees of endemism at all taxonomic levels. The waters surrounding Madagascar are still poorly studied for benthic marine diatom assemblages. The aim of our study was to analyse the taxonomic biodiversity and molecular phylogeny of the diatom assemblages of the Nosy Be archipelago in terms of their ubiquity/rarity. A total of 65 samples from 14 sampling sites were collected from the Nosy Be and Nosy Tanikely islands in June/July 2014. Diatom identification, based on light and electron microscopic examination, revealed 332 diatom taxa. From those, ∼90 strains were isolated and successfully cultured. So far, DNA has been extracted and one chloroplast gene (<i>rbc</i>L) sequenced from 21 strains in order to reconstruct their single gene phylogeny. Taxa selected for sequencing and the preliminary phylogeny are from genera considered common benthic and planktonic forms in the marine littoral: <i>Bellerochea</i>, <i>Melosira</i>, <i>Paralia</i>, <i>Triceratium</i>, <i>Navicula</i>, <i>Hippodonta</i>, <i>Auricula</i>, <i>Nitzschia</i>, <i>Cocconeis</i>, and some araphids. For each site, Shannon and Simpson biodiversity indices were calculated. Diatom assemblages of Nosy Be were characterized by high biodiversity. The most abundant species was <i>Cocconeis scutellum</i>. Whereas some of the cultured taxa seem to represent cosmopolitan or zonal forms: e.g., <i>Bellerochea malleus</i>, <i>Triceratium dubium</i>, <i>Melosira lineata</i>, <i>Paralia crawfordii</i>, or <i>Nitzschia inconspicua</i>, most of the taxa isolated in culture and sequenced have not been observed in earlier works based on use of molecular markers. The dominant taxa were generally those with broad geographic distribution with global/Indo-Pacific affinities. Amongst all 332 taxa, only 35% have been identified to the species level. Many of the taxa identified to the generic level may turn out to represent taxa new to science after further SEM examination and DNA sequencing.
DOI: 10.6084/m9.figshare.11440398
title: Predator-induced maternal and paternal effects independently alter sexual selection

description: Abstract:Parental experience alters survival-related phenotypes of offspring in both adaptive and non-adaptive ways, yielding rapid inter- and transgenerational fitness effects. Yet, fitness comprises survival and reproduction, and parental effects on mating decisions could alter the strength and direction of sexual selection, affecting long-term evolutionary trajectories. We used a full factorial design in which threespine stickleback (<i>Gasterosteus aculeatus</i>) mothers, fathers, both, or neither were exposed to a model predator at developmentally appropriate times to test for predator-induced maternal, paternal, and joint parental effects on daughters’ mating behavior. We tested the responsiveness, preferences, and mate choices of adult daughters in no-choice trials with wild-caught males who had varied sexual signals. Maternal and paternal predator exposure independently yielded daughters who preferred males who were intermediate in conspicuousness (with duller nuptial coloration and who courted less vigorously), relaxing the typical preference for the most conspicuous males. The combined effects of maternal and paternal predator exposure were not cumulative; when both parents were predator-exposed, single-parent effects on mate preferences were reversed. Thus, we cannot assume that maternal and paternal effects additively combine to produce "parental" effects. Further, joint parental predator exposure yielded daughters who were three times less likely to mate at all. Stress-induced intergenerational parental effects on reproductive decisions like those observed here may potentiate rapid transgenerational responses to novel and changing mating environments.<br>
DOI: 10.6084/m9.figshare.11440404.v1
title: Marine diatom assemblages of the Nosy Be Island coasts, NW Madagascar: species composition and biodiversity using molecular and morphological taxonomy

description: Madagascar is an isolated oceanic island characterized by high degrees of endemism at all taxonomic levels. The waters surrounding Madagascar are still poorly studied for benthic marine diatom assemblages. The aim of our study was to analyse the taxonomic biodiversity and molecular phylogeny of the diatom assemblages of the Nosy Be archipelago in terms of their ubiquity/rarity. A total of 65 samples from 14 sampling sites were collected from the Nosy Be and Nosy Tanikely islands in June/July 2014. Diatom identification, based on light and electron microscopic examination, revealed 332 diatom taxa. From those, ∼90 strains were isolated and successfully cultured. So far, DNA has been extracted and one chloroplast gene (<i>rbc</i>L) sequenced from 21 strains in order to reconstruct their single gene phylogeny. Taxa selected for sequencing and the preliminary phylogeny are from genera considered common benthic and planktonic forms in the marine littoral: <i>Bellerochea</i>, <i>Melosira</i>, <i>Paralia</i>, <i>Triceratium</i>, <i>Navicula</i>, <i>Hippodonta</i>, <i>Auricula</i>, <i>Nitzschia</i>, <i>Cocconeis</i>, and some araphids. For each site, Shannon and Simpson biodiversity indices were calculated. Diatom assemblages of Nosy Be were characterized by high biodiversity. The most abundant species was <i>Cocconeis scutellum</i>. Whereas some of the cultured taxa seem to represent cosmopolitan or zonal forms: e.g., <i>Bellerochea malleus</i>, <i>Triceratium dubium</i>, <i>Melosira lineata</i>, <i>Paralia crawfordii</i>, or <i>Nitzschia inconspicua</i>, most of the taxa isolated in culture and sequenced have not been observed in earlier works based on use of molecular markers. The dominant taxa were generally those with broad geographic distribution with global/Indo-Pacific affinities. Amongst all 332 taxa, only 35% have been identified to the species level. Many of the taxa identified to the generic level may turn out to represent taxa new to science after further SEM examination and DNA sequencing.
DOI: 10.6084/m9.figshare.11440398.v1
title: Predator-induced maternal and paternal effects independently alter sexual selection

description: Abstract:Parental experience alters survival-related phenotypes of offspring in both adaptive and non-adaptive ways, yielding rapid inter- and transgenerational fitness effects. Yet, fitness comprises survival and reproduction, and parental effects on mating decisions could alter the strength and direction of sexual selection, affecting long-term evolutionary trajectories. We used a full factorial design in which threespine stickleback (<i>Gasterosteus aculeatus</i>) mothers, fathers, both, or neither were exposed to a model predator at developmentally appropriate times to test for predator-induced maternal, paternal, and joint parental effects on daughters’ mating behavior. We tested the responsiveness, preferences, and mate choices of adult daughters in no-choice trials with wild-caught males who had varied sexual signals. Maternal and paternal predator exposure independently yielded daughters who preferred males who were intermediate in conspicuousness (with duller nuptial coloration and who courted less vigorously), relaxing the typical preference for the most conspicuous males. The combined effects of maternal and paternal predator exposure were not cumulative; when both parents were predator-exposed, single-parent effects on mate preferences were reversed. Thus, we cannot assume that maternal and paternal effects additively combine to produce "parental" effects. Further, joint parental predator exposure yielded daughters who were three times less likely to mate at all. Stress-induced intergenerational parental effects on reproductive decisions like those observed here may potentiate rapid transgenerational responses to novel and changing mating environments.<br>
DOI: 10.6084/m9.figshare.11440395
title: Are there any true marine <i>Chlorella</i> species? Molecular phylogenetic assessment and ecology of marine <i>Chlorella</i>-like organisms, including a description of <i>Droopiella</i> gen. nov.

description: Green algal species of spherical cell shape are generally considered to belong to the genus <i>Chlorella</i>, which are mostly freshwater or terrestrial organisms. Phylogenetic studies have shown that this genus is polyphyletic and belongs to different classes. However, until now, only freshwater or terrestrial strains have been studied. Here we investigated 11 strains of ‘marine’ <i>Chlorella</i> deposited in public culture collections, which we studied using an integrative approach. These strains were largely isolated from marine rock pools and brackish estuaries. SSU and ITS regions of the nuclear encoded ribosomal DNA were sequenced, ribosomal secondary structures were analysed and cell morphology, salinity tolerance and reproduction were examined. Our results showed that the marine strains are also of polyphyletic origin. Surprisingly, three marine isolates belong to <i>Chlorella vulgaris</i> according to the phylogenetic analyses, but showed a high phenotypic plasticity. Whereas these strains showed the typical morphology of <i>C. vulgaris</i> under freshwater conditions, they increased the cell shape and formed cell packages under marine conditions. In contrast, the other investigated strains showed no changes after changing the media. Two of the investigated strains belong to the genus <i>Chloroidium</i>, and those remaining represent a new genus, <i>Droopiella</i>.
DOI: 10.6084/m9.figshare.11440392
title: Supplementary Figures from Method for controlling vibration by exploiting piecewise-linear nonlinearity in energy harvesters

description: Vibration energy is becoming a significant alternative solution for energy generation. Recently, a great deal of research has been conducted on how to harvest energy from vibration sources ranging from ocean waves to human motion to microsystems. In this paper, a theoretical model of a piecewise-linear (PWL) nonlinear vibration harvester that has potential applications in variety of fields is proposed and numerically investigated. This new technique enables automatic frequency tunability in the energy harvester by controlling the gap size in the PWL oscillator so that it is able to adapt to changes in excitations. To optimize the performance of the proposed system, a control method combining the response prediction, signal measurement and gap adjustment mechanism is proposed in this paper. This new energy harvester not only overcomes the limitation of traditional linear energy harvesters that can only provide the maximum power generation efficiency over a narrow frequency range but also improves the performance of current nonlinear energy harvesters that are not as efficient as linear energy harvesters at resonance. The proposed system is demonstrated in several case studies to illustrate its effectiveness for a number of different excitations.
DOI: 10.6084/m9.figshare.11440395.v1
title: Are there any true marine <i>Chlorella</i> species? Molecular phylogenetic assessment and ecology of marine <i>Chlorella</i>-like organisms, including a description of <i>Droopiella</i> gen. nov.

description: Green algal species of spherical cell shape are generally considered to belong to the genus <i>Chlorella</i>, which are mostly freshwater or terrestrial organisms. Phylogenetic studies have shown that this genus is polyphyletic and belongs to different classes. However, until now, only freshwater or terrestrial strains have been studied. Here we investigated 11 strains of ‘marine’ <i>Chlorella</i> deposited in public culture collections, which we studied using an integrative approach. These strains were largely isolated from marine rock pools and brackish estuaries. SSU and ITS regions of the nuclear encoded ribosomal DNA were sequenced, ribosomal secondary structures were analysed and cell morphology, salinity tolerance and reproduction were examined. Our results showed that the marine strains are also of polyphyletic origin. Surprisingly, three marine isolates belong to <i>Chlorella vulgaris</i> according to the phylogenetic analyses, but showed a high phenotypic plasticity. Whereas these strains showed the typical morphology of <i>C. vulgaris</i> under freshwater conditions, they increased the cell shape and formed cell packages under marine conditions. In contrast, the other investigated strains showed no changes after changing the media. Two of the investigated strains belong to the genus <i>Chloroidium</i>, and those remaining represent a new genus, <i>Droopiella</i>.
DOI: 10.6084/m9.figshare.11440380
title: Dataset_Legionella_2019

description: A field experiment with single factor design was used. We compared four official messages communicated online by the municipality of Evergem. The communication team of the municipality provided us with all of the messages they had communicated to the public. We selected four that were representative of their overall communication and which differed in terms of content and format. For this study, we gathered data from local residents in and around the affected area. A total of 529 respondents took part in the experiment. Two sampling methods were used to gather participants. First, a market research company recruited a representative sample of 387 inhabitants of the Ghent Canal Zone and its immediate environment. In addition, a convenience sample of 142 respondents took part in the study. These respondents were recruited by posting the survey link on Facebook and Twitter, mainly in online communities for members living in the affected area.
DOI: 10.6084/m9.figshare.11440392.v1
title: Supplementary Figures from Method for controlling vibration by exploiting piecewise-linear nonlinearity in energy harvesters

description: Vibration energy is becoming a significant alternative solution for energy generation. Recently, a great deal of research has been conducted on how to harvest energy from vibration sources ranging from ocean waves to human motion to microsystems. In this paper, a theoretical model of a piecewise-linear (PWL) nonlinear vibration harvester that has potential applications in variety of fields is proposed and numerically investigated. This new technique enables automatic frequency tunability in the energy harvester by controlling the gap size in the PWL oscillator so that it is able to adapt to changes in excitations. To optimize the performance of the proposed system, a control method combining the response prediction, signal measurement and gap adjustment mechanism is proposed in this paper. This new energy harvester not only overcomes the limitation of traditional linear energy harvesters that can only provide the maximum power generation efficiency over a narrow frequency range but also improves the performance of current nonlinear energy harvesters that are not as efficient as linear energy harvesters at resonance. The proposed system is demonstrated in several case studies to illustrate its effectiveness for a number of different excitations.
DOI: 10.6084/m9.figshare.11440380.v1
title: Dataset_Legionella_2019

description: A field experiment with single factor design was used. We compared four official messages communicated online by the municipality of Evergem. The communication team of the municipality provided us with all of the messages they had communicated to the public. We selected four that were representative of their overall communication and which differed in terms of content and format. For this study, we gathered data from local residents in and around the affected area. A total of 529 respondents took part in the experiment. Two sampling methods were used to gather participants. First, a market research company recruited a representative sample of 387 inhabitants of the Ghent Canal Zone and its immediate environment. In addition, a convenience sample of 142 respondents took part in the study. These respondents were recruited by posting the survey link on Facebook and Twitter, mainly in online communities for members living in the affected area.
DOI: 10.6084/m9.figshare.11440380.v2
title: Dataset_Legionella_2019

description: A field experiment with single factor design was used. We compared four official messages communicated online by the municipality of Evergem. The communication team of the municipality provided us with all of the messages they had communicated to the public. We selected four that were representative of their overall communication and which differed in terms of content and format. For this study, we gathered data from local residents in and around the affected area. A total of 529 respondents took part in the experiment. Two sampling methods were used to gather participants. First, a market research company recruited a representative sample of 387 inhabitants of the Ghent Canal Zone and its immediate environment. In addition, a convenience sample of 142 respondents took part in the study. These respondents were recruited by posting the survey link on Facebook and Twitter, mainly in online communities for members living in the affected area.