DOI: 10.5061/dryad.8ns67
title: Data from: Parallel effects of the inversion In(3R)Payne on body size across the North American and Australian clines in Drosophila melanogaster

description: Chromosomal inversions are thought to play a major role in climatic
 adaptation. In D. melanogaster, the cosmopolitan inversion In(3R)Payne
 exhibits latitudinal clines on multiple continents. Since many fitness
 traits show similar clines, it is tempting to hypothesize that In(3R)P
 underlies observed clinal patterns for some of these traits. In support of
 this idea, previous work in Australian populations has demonstrated that
 In(3R)P affects body size but not development time or cold resistance.
 However, similar data from other clines of this inversion are largely
 lacking; finding parallel effects of In(3R)P across multiple clines would
 considerably strengthen the case for clinal selection. Here, we have
 analyzed the phenotypic effects of In(3R)P in populations originating from
 the endpoints of the latitudinal cline along the North American east
 coast. We measured development time, egg-to-adult survival, several
 size-related traits (femur and tibia length, wing area and shape), chill
 coma recovery, oxidative stress resistance and triglyceride content in
 homokaryon lines carrying In(3R)P or the standard arrangement. Our central
 finding is that the effects of In(3R)P along the North American cline
 match those observed in Australia: standard arrangement lines were larger
 than inverted lines, but the inversion did not influence development time
 or cold resistance. Similarly, In(3R)P did not affect egg-to-adult
 survival, oxidative stress resistance and lipid content. In(3R)P thus
 seems to specifically affect size traits in populations from both
 continents. This parallelism strongly suggests an adaptive pattern,
 whereby the inversion has captured alleles associated with growth
 regulation and clinal selection acts on size across both continents.
description: Data_Kapun_JEBExplanation of raw data: We measured development time (measured in h), egg-to-adult survival (proportion viability), chill coma recovery time (measured in h), oxidative stress resistance (age at death, measured in h), triglyceride content (measured in µg), wing size (measured in mm2), femur and tibia length (measured in mm), allometry differences (ratios of femur length to tibia length, (2) femur length versus wing area, and (3) tibia length versus wing area), Jacobian determinant values of wing shape differences (for males and females separately) for isochromosomal lines of D. melanogaster from Florida and Maine that were either homokaryotypic for the In(3R)Payne inverted arrangement or for the standard chromosomal arrangement. Our main goal was to analyze how traits covary with inversion karyotype (inverted versus standard arrangement) and geography (Florida versus Maine). Here we give the raw data for each trait as well as line means (see the worksheet "Summary"). For more details see our paper Kapun et al. (2016, "Parallel effects of the inversion In(3R)Payne on body size across the North American and Australian clines in Drosophila melanogaster", Journal of Evolutionary Biology). For additional details please contact the first author, Martin Kapun (martin.kapunl@unil.ch), or the corresponding author, Thomas Flatt (thomas.flatt@unil.ch).