cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.1569533.v1
title: Evaluation of a newly developed quantitative heart-type fatty acid binding protein assay based on fluorescence immunochromatography using specific monoclonal antibodies

description: <b><i>Objectives.</i></b> To develop a rapid, sensitive and specific assay for quantification of serum heart-type fatty acid binding protein (H-FABP) based on immunofluorescence of specific monoclonal antibodies. <b><i>Design and Methods.</i></b> We generated novel H-FABP-directed monoclonal antibodies by cloning of spleen cells of mice immunized with H-FABP. Epitopes were mapped and antigen affinity was assessed by surface plasmon resonance (SPR). The H-FABP specific monoclonal antibodies were coupled to fluorescent beads and sprayed onto a nitrocellulose membrane facilitating quantification of H-FABP by immunofluorescence. Reagent cross-reactivity, interference resistance, accuracy and sensitivity were examined. A total of 103 clinical samples were used to compare the sensitivity and specificity of the new assay to a commercially available Randox kit. <b><i>Results.</i></b> This new assay could be finished within 15 min, with sensitivity reaching 1 ng/ml. In a trial of 103 clinical serum samples, the new testing kit results were highly correlated with those from the Randox kit (R<sup>2</sup> = 0.9707). Using the Randox kit as the reference kit, the sensitivity of the new assay was 98.25%, and specificity was 100%. <b><i>Conclusions.</i></b> An immunofluorescence-based H-FABP assay employing novel monoclonal antibodies could rapidly, specifically and sensitively detect H-FABP in serum samples, providing an effective method for rapid clinical assessment of H-FABP index in the clinic.
DOI: 10.6084/m9.figshare.1569513
title: Phylogeographic patterns in suckermouth catfish <i>Hypostomus ancistroides</i> (Loricariidae): dispersion, vicariance and species complexity across a Neotropical biogeographic region

description: The upper Paraná River system (UP) is a highly diverse biogeographic province for freshwater fishes, but little is known about processes which shaped that diversity. This study describes the phylogeographic pattern in <i>Hypostomus ancistroides</i>, a suckermouth catfish species that is widespread in the UP and also reported from the adjoining Ribeira do Iguape basin. We used complete mtDNA sequences of ATPase 6/8 of 162 specimens to infer haplotype distribution using phylogenetic and demographic analyses and a Bayesian molecular clock. Results suggest that during the Quaternary <i>H. ancistroides</i> has undergone superimposed phylogeographic histories, alternating between isolation and subsequent merging of different populations. Occurrence of an isolated population on the Ribeira de Iguape is demonstrated to be a Pleistocene headwater capture event. Widely distributed haplotypes indicate deep genetic differences and suggest that populations of <i>H. ancistroides</i> were isolated for considerable time, but did not undergo speciation because of recurrent population mixing.
DOI: 10.6084/m9.figshare.1569513.v1
title: Phylogeographic patterns in suckermouth catfish <i>Hypostomus ancistroides</i> (Loricariidae): dispersion, vicariance and species complexity across a Neotropical biogeographic region

description: The upper Paraná River system (UP) is a highly diverse biogeographic province for freshwater fishes, but little is known about processes which shaped that diversity. This study describes the phylogeographic pattern in <i>Hypostomus ancistroides</i>, a suckermouth catfish species that is widespread in the UP and also reported from the adjoining Ribeira do Iguape basin. We used complete mtDNA sequences of ATPase 6/8 of 162 specimens to infer haplotype distribution using phylogenetic and demographic analyses and a Bayesian molecular clock. Results suggest that during the Quaternary <i>H. ancistroides</i> has undergone superimposed phylogeographic histories, alternating between isolation and subsequent merging of different populations. Occurrence of an isolated population on the Ribeira de Iguape is demonstrated to be a Pleistocene headwater capture event. Widely distributed haplotypes indicate deep genetic differences and suggest that populations of <i>H. ancistroides</i> were isolated for considerable time, but did not undergo speciation because of recurrent population mixing.
DOI: 10.6084/m9.figshare.1569506
title: Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats

description: Age related decline in reproductive performance in women is well documented and apoptosis has been considered as one of the reasons for the decline of primordial follicle reserve. Recently we observed a decline in the efficiency of DNA repair ability in aged rat primordial follicles as demonstrated by decreased mRNA levels of DNA repair genes <i>BRCA1 </i>and<i> H2AX</i>. In the present study, a two-dimensional electrophoresis (2DE) proteomic approach was employed to identify differentially expressed proteins in primordial follicles isolated from ovaries of immature (∼20 days) and aged (∼400–450 days) rats. Using MALDI-TOF/TOF MS, we identified 13 differentially expressed proteins (<i>p</i>
DOI: 10.6084/m9.figshare.1569505
title: Enhancing Estimation for Interest Rate Diffusion Models With Bond Prices

description: We consider improving estimating parameters of diffusion processes for interest rates by incorporating information in bond prices. This is designed to improve the estimation of the drift parameters, which are known to be subject to large estimation errors. It is shown that having the bond prices together with the short rates leads to more efficient estimation of all parameters for the interest rate models. It enhances the estimation efficiency of the maximum likelihood estimation based on the interest rate dynamics alone. The combined estimation based on the bond prices and the interest rate dynamics can also provide inference to the risk premium parameter. Simulation experiments were conducted to confirm the theoretical properties of the estimators concerned. We analyze the overnight Fed fund rates together with the U.S. Treasury bond prices. Supplementary materials for this article are available online.
DOI: 10.6084/m9.figshare.1569512.v2
title: Comparison of transhumeral socket designs utilizing patient assessment and <i>in vivo</i> skeletal and socket motion tracking: a case study

description: <i>Purpose</i>: This case study compares the impact of two prosthetic socket designs, a “traditional” transhumeral (TH) socket design and a Compression Released Stabilized (CRS) socket. <i>Methods</i>: A CRS socket was compared to the existing socket of two persons with transhumeral amputation. Comparisons included assessments of patient comfort and satisfaction with fit, as well as dynamic kinematic assessment using a novel high-speed, high-resolution, bi-plane video radiography system (XROMM, for X-ray Reconstruction of Moving Morphology). <i>Results</i>: Subjects were more satisfied with the comfort of the traditional sockets, although they had positive impressions about aspects of the fit and style of the CRS socket, and thought that it provided better control. Dynamic kinematic assessment revealed that the CRS socket provided better control of the residual limb within the socket, and had less slippage as compared to a traditional TH socket design. <i>Conclusions</i>: The TH CRS socket provided better control of the residual limb within the socket, and had less slippage. However, participants were less satisfied with the comfort and overall utility of the CRS socket, and stated that additional fitting visits/modifications to the CRS socket were needed. It is possible that satisfaction with the CRS socket may have improved with prosthetic adjustment and more acclimation time.Implications for RehabilitationA comfortable, good fitting prosthetic socket is the key factor in determining how long (or if) an upper limb amputee can tolerate wearing a prosthesis.This case series was a comparison of two socket designs, a 'traditional' socket design and a Compression Released Stabilized (CRS) socket design in persons with transhumeral amputation.The CRS socket provided better control of the residual limb within the socket, and had less slippage. However, its tightness made it more difficult to don.Both subjects were less satisfied with the comfort and overall utility of the CRS socket. However, satisfaction might have been improved with additional fitting visits and more acclimation time. A comfortable, good fitting prosthetic socket is the key factor in determining how long (or if) an upper limb amputee can tolerate wearing a prosthesis. This case series was a comparison of two socket designs, a 'traditional' socket design and a Compression Released Stabilized (CRS) socket design in persons with transhumeral amputation. The CRS socket provided better control of the residual limb within the socket, and had less slippage. However, its tightness made it more difficult to don. Both subjects were less satisfied with the comfort and overall utility of the CRS socket. However, satisfaction might have been improved with additional fitting visits and more acclimation time.
DOI: 10.6084/m9.figshare.1569512
title: Comparison of transhumeral socket designs utilizing patient assessment and <i>in vivo</i> skeletal and socket motion tracking: a case study

description: <i>Purpose</i>: This case study compares the impact of two prosthetic socket designs, a “traditional” transhumeral (TH) socket design and a Compression Released Stabilized (CRS) socket. <i>Methods</i>: A CRS socket was compared to the existing socket of two persons with transhumeral amputation. Comparisons included assessments of patient comfort and satisfaction with fit, as well as dynamic kinematic assessment using a novel high-speed, high-resolution, bi-plane video radiography system (XROMM, for X-ray Reconstruction of Moving Morphology). <i>Results</i>: Subjects were more satisfied with the comfort of the traditional sockets, although they had positive impressions about aspects of the fit and style of the CRS socket, and thought that it provided better control. Dynamic kinematic assessment revealed that the CRS socket provided better control of the residual limb within the socket, and had less slippage as compared to a traditional TH socket design. <i>Conclusions</i>: The TH CRS socket provided better control of the residual limb within the socket, and had less slippage. However, participants were less satisfied with the comfort and overall utility of the CRS socket, and stated that additional fitting visits/modifications to the CRS socket were needed. It is possible that satisfaction with the CRS socket may have improved with prosthetic adjustment and more acclimation time.Implications for RehabilitationA comfortable, good fitting prosthetic socket is the key factor in determining how long (or if) an upper limb amputee can tolerate wearing a prosthesis.This case series was a comparison of two socket designs, a 'traditional' socket design and a Compression Released Stabilized (CRS) socket design in persons with transhumeral amputation.The CRS socket provided better control of the residual limb within the socket, and had less slippage. However, its tightness made it more difficult to don.Both subjects were less satisfied with the comfort and overall utility of the CRS socket. However, satisfaction might have been improved with additional fitting visits and more acclimation time. A comfortable, good fitting prosthetic socket is the key factor in determining how long (or if) an upper limb amputee can tolerate wearing a prosthesis. This case series was a comparison of two socket designs, a 'traditional' socket design and a Compression Released Stabilized (CRS) socket design in persons with transhumeral amputation. The CRS socket provided better control of the residual limb within the socket, and had less slippage. However, its tightness made it more difficult to don. Both subjects were less satisfied with the comfort and overall utility of the CRS socket. However, satisfaction might have been improved with additional fitting visits and more acclimation time.
DOI: 10.6084/m9.figshare.1569512.v1
title: Comparison of transhumeral socket designs utilizing patient assessment and <i>in vivo</i> skeletal and socket motion tracking: a case study

description: <i>Purpose</i>: This case study compares the impact of two prosthetic socket designs, a “traditional” transhumeral (TH) socket design and a Compression Released Stabilized (CRS) socket. <i>Methods</i>: A CRS socket was compared to the existing socket of two persons with transhumeral amputation. Comparisons included assessments of patient comfort and satisfaction with fit, as well as dynamic kinematic assessment using a novel high-speed, high-resolution, bi-plane video radiography system (XROMM, for X-ray Reconstruction of Moving Morphology). <i>Results</i>: Subjects were more satisfied with the comfort of the traditional sockets, although they had positive impressions about aspects of the fit and style of the CRS socket, and thought that it provided better control. Dynamic kinematic assessment revealed that the CRS socket provided better control of the residual limb within the socket, and had less slippage as compared to a traditional TH socket design. <i>Conclusions</i>: The TH CRS socket provided better control of the residual limb within the socket, and had less slippage. However, participants were less satisfied with the comfort and overall utility of the CRS socket, and stated that additional fitting visits/modifications to the CRS socket were needed. It is possible that satisfaction with the CRS socket may have improved with prosthetic adjustment and more acclimation time.Implications for RehabilitationA comfortable, good fitting prosthetic socket is the key factor in determining how long (or if) an upper limb amputee can tolerate wearing a prosthesis.This case series was a comparison of two socket designs, a 'traditional' socket design and a Compression Released Stabilized (CRS) socket design in persons with transhumeral amputation.The CRS socket provided better control of the residual limb within the socket, and had less slippage. However, its tightness made it more difficult to don.Both subjects were less satisfied with the comfort and overall utility of the CRS socket. However, satisfaction might have been improved with additional fitting visits and more acclimation time. A comfortable, good fitting prosthetic socket is the key factor in determining how long (or if) an upper limb amputee can tolerate wearing a prosthesis. This case series was a comparison of two socket designs, a 'traditional' socket design and a Compression Released Stabilized (CRS) socket design in persons with transhumeral amputation. The CRS socket provided better control of the residual limb within the socket, and had less slippage. However, its tightness made it more difficult to don. Both subjects were less satisfied with the comfort and overall utility of the CRS socket. However, satisfaction might have been improved with additional fitting visits and more acclimation time.
DOI: 10.6084/m9.figshare.1569506.v2
title: Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats

description: Age related decline in reproductive performance in women is well documented and apoptosis has been considered as one of the reasons for the decline of primordial follicle reserve. Recently we observed a decline in the efficiency of DNA repair ability in aged rat primordial follicles as demonstrated by decreased mRNA levels of DNA repair genes <i>BRCA1 </i>and<i> H2AX</i>. In the present study, a two-dimensional electrophoresis (2DE) proteomic approach was employed to identify differentially expressed proteins in primordial follicles isolated from ovaries of immature (∼20 days) and aged (∼400–450 days) rats. Using MALDI-TOF/TOF MS, we identified 13 differentially expressed proteins (<i>p</i>
DOI: 10.6084/m9.figshare.1569505.v2
title: Enhancing Estimation for Interest Rate Diffusion Models With Bond Prices

description: We consider improving estimating parameters of diffusion processes for interest rates by incorporating information in bond prices. This is designed to improve the estimation of the drift parameters, which are known to be subject to large estimation errors. It is shown that having the bond prices together with the short rates leads to more efficient estimation of all parameters for the interest rate models. It enhances the estimation efficiency of the maximum likelihood estimation based on the interest rate dynamics alone. The combined estimation based on the bond prices and the interest rate dynamics can also provide inference to the risk premium parameter. Simulation experiments were conducted to confirm the theoretical properties of the estimators concerned. We analyze the overnight Fed fund rates together with the U.S. Treasury bond prices. Supplementary materials for this article are available online.
DOI: 10.6084/m9.figshare.1569506.v1
title: Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats

description: Age related decline in reproductive performance in women is well documented and apoptosis has been considered as one of the reasons for the decline of primordial follicle reserve. Recently we observed a decline in the efficiency of DNA repair ability in aged rat primordial follicles as demonstrated by decreased mRNA levels of DNA repair genes <i>BRCA1 </i>and<i> H2AX</i>. In the present study, a two-dimensional electrophoresis (2DE) proteomic approach was employed to identify differentially expressed proteins in primordial follicles isolated from ovaries of immature (∼20 days) and aged (∼400–450 days) rats. Using MALDI-TOF/TOF MS, we identified 13 differentially expressed proteins (<i>p</i>
DOI: 10.6084/m9.figshare.1569497
title: What Teachers Should Know About the Bootstrap: Resampling in the Undergraduate Statistics Curriculum

description: Bootstrapping has enormous potential in statistics education and practice, but there are subtle issues and ways to go wrong. For example, the common combination of nonparametric bootstrapping and bootstrap percentile confidence intervals is less accurate than using <i>t</i>-intervals for small samples, though more accurate for larger samples. My goals in this article are to provide a deeper understanding of bootstrap methods—how they work, when they work or not, and which methods work better—and to highlight pedagogical issues. Supplementary materials for this article are available online. [Received December 2014. Revised August 2015]
DOI: 10.6084/m9.figshare.1569495
title: Regulation of the chitin degradation and utilization system by the ChiX small RNA in <i>Serratia marcescens</i> 2170

description: <i>Serratia marcescens</i> 2170 produces three different types of chitinases and chitin-binding protein CBP21. We found that transposon insertion into the 5′ untranslated region (5′ UTR) of <i>chiPQ</i>-<i>ctb</i> led to defective chitinase and CBP21 production. ChiX small RNA possessed the complementary sequence of the 5′ UTRs of the <i>chiPQ</i>-<i>ctb</i> and <i>chiR</i> and repressed the expression of <i>chiP</i> and <i>chiR</i>. ChiX was detected in a medium containing glucose, glycerol, GlcNAc, and (GlcNAc)<sub>2</sub>, but the expression of both <i>chiP</i> and <i>chiR</i> was only observed in a medium containing (GlcNAc)<sub>2</sub>. ∆<i>chiX</i> mutant produced chitinases, CBP21, and chitobiase without induction. <i>chiP</i> transcripts were more abundant than those of <i>chiR</i> or <i>chiX</i> in a medium containing (GlcNAc)<sub>2</sub>. These results suggest that the constitutively expressed ChiX binds to the highly abundant <i>chiP</i> 5′ UTR, thereby leading to the induction of <i>chiR</i> mRNA translation and the subsequent expression of chitinases and CBP21.
DOI: 10.6084/m9.figshare.1569494
title: Dextran-5-(4-ethoxycarbonylphenylazo)salicylic acid ester, a polymeric colon-specific prodrug releasing 5-aminosalicylic acid and benzocaine, ameliorates TNBS-induced rat colitis

description: Local anesthetics have beneficial effects on colitis. Dextran-5-(4-ethoxycarbonylphenylazo)salicylic acid ester (Dex-5-ESA), designed as a polymeric colon-specific prodrug liberating 5-ASA and benzocaine in the large intestine, was prepared and its therapeutic activity against colitis was evaluated using a TNBS-induced rat colitis model. Dex-5-ESA liberated 5-ASA and benzocaine in the cecal contents while (bio)chemically stable in the small intestinal contents and mucosa. Oral administration of Dex-5-ESA (equivalent to 10 mg 5-ASA/kg, twice a day) alleviated colonic injury and reduced MPO activity in the inflamed colon. In parallel, pro-inflammatory mediators, COX-2, iNOS and CINC-3, elevated by TNBS-induced colitis, were substantially diminished in the inflamed colon. Dex-5-ESA was much more effective for the treatment of colitis than 5-(4-ethoxycarbonylphenylazo)salicylic acid (5-ESA) that may not deliver benzocaine to the large intestine. Our data suggest that Dex-5-ESA is a polymeric colon-specific prodrug, liberating 5-ASA and benzocaine in the target site (large intestine), probably exerting anti-colitic effects by combined action of 5-ASA and benzocaine.
DOI: 10.6084/m9.figshare.1569494.v1
title: Dextran-5-(4-ethoxycarbonylphenylazo)salicylic acid ester, a polymeric colon-specific prodrug releasing 5-aminosalicylic acid and benzocaine, ameliorates TNBS-induced rat colitis

description: Local anesthetics have beneficial effects on colitis. Dextran-5-(4-ethoxycarbonylphenylazo)salicylic acid ester (Dex-5-ESA), designed as a polymeric colon-specific prodrug liberating 5-ASA and benzocaine in the large intestine, was prepared and its therapeutic activity against colitis was evaluated using a TNBS-induced rat colitis model. Dex-5-ESA liberated 5-ASA and benzocaine in the cecal contents while (bio)chemically stable in the small intestinal contents and mucosa. Oral administration of Dex-5-ESA (equivalent to 10 mg 5-ASA/kg, twice a day) alleviated colonic injury and reduced MPO activity in the inflamed colon. In parallel, pro-inflammatory mediators, COX-2, iNOS and CINC-3, elevated by TNBS-induced colitis, were substantially diminished in the inflamed colon. Dex-5-ESA was much more effective for the treatment of colitis than 5-(4-ethoxycarbonylphenylazo)salicylic acid (5-ESA) that may not deliver benzocaine to the large intestine. Our data suggest that Dex-5-ESA is a polymeric colon-specific prodrug, liberating 5-ASA and benzocaine in the target site (large intestine), probably exerting anti-colitic effects by combined action of 5-ASA and benzocaine.
DOI: 10.6084/m9.figshare.1569495.v1
title: Regulation of the chitin degradation and utilization system by the ChiX small RNA in <i>Serratia marcescens</i> 2170

description: <i>Serratia marcescens</i> 2170 produces three different types of chitinases and chitin-binding protein CBP21. We found that transposon insertion into the 5′ untranslated region (5′ UTR) of <i>chiPQ</i>-<i>ctb</i> led to defective chitinase and CBP21 production. ChiX small RNA possessed the complementary sequence of the 5′ UTRs of the <i>chiPQ</i>-<i>ctb</i> and <i>chiR</i> and repressed the expression of <i>chiP</i> and <i>chiR</i>. ChiX was detected in a medium containing glucose, glycerol, GlcNAc, and (GlcNAc)<sub>2</sub>, but the expression of both <i>chiP</i> and <i>chiR</i> was only observed in a medium containing (GlcNAc)<sub>2</sub>. ∆<i>chiX</i> mutant produced chitinases, CBP21, and chitobiase without induction. <i>chiP</i> transcripts were more abundant than those of <i>chiR</i> or <i>chiX</i> in a medium containing (GlcNAc)<sub>2</sub>. These results suggest that the constitutively expressed ChiX binds to the highly abundant <i>chiP</i> 5′ UTR, thereby leading to the induction of <i>chiR</i> mRNA translation and the subsequent expression of chitinases and CBP21.