cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6207917
title: DNA content estimation of Fig and Black Mulberry using flow cytometry

description: Abstract In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.
DOI: 10.6084/m9.figshare.6207917.v1
title: DNA content estimation of Fig and Black Mulberry using flow cytometry

description: Abstract In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.
DOI: 10.6084/m9.figshare.6207914
title: Effects of black seed (Nigella sativa), ginger (Zingiber officinale) and cone flower (Echinacea angustifolia) on the immune system of rainbow trout, Oncorhynchus mykiss

description: ABSTRACT This study was carried out to evaluate the effects of dietary essential oils of ginger (Zingiber officinale), black seed (Nigella sativa) and cone flower (Echinacea angustifolia) on the immune system of cultured rainbow trout, Oncorhynchus mykiss. A total of 360 fish were divided into 4 groups and 3 replicates each containing 30 fish. The fish were fed for 21 days with 1% of the herbal essential oils and with unsupplemented diet as the control group. Haematological and immune parameters including serum lysozyme, number of phagocytic bacteria, Hb, PCV, MCV, MCH, MCHC, number of RBC and WBC were studied. Treatments recorded enhancement in immune parameters compared to the control group. Significantly higher serum lysozyme level and phagocytic germs were detected in the groups fed black seed, ginger and cone flower (P&lt;0.05). However, no significant difference was found in RBC, Hb, PCV, MCV, MCH and MCHC of fish fed the diets containing essential oils and the control group (P&gt;0.05). The results of this study demonstrated that essential oils might strengthen the non-specific immunity of rainbow trout.
DOI: 10.6084/m9.figshare.6207914.v1
title: Effects of black seed (Nigella sativa), ginger (Zingiber officinale) and cone flower (Echinacea angustifolia) on the immune system of rainbow trout, Oncorhynchus mykiss

description: ABSTRACT This study was carried out to evaluate the effects of dietary essential oils of ginger (Zingiber officinale), black seed (Nigella sativa) and cone flower (Echinacea angustifolia) on the immune system of cultured rainbow trout, Oncorhynchus mykiss. A total of 360 fish were divided into 4 groups and 3 replicates each containing 30 fish. The fish were fed for 21 days with 1% of the herbal essential oils and with unsupplemented diet as the control group. Haematological and immune parameters including serum lysozyme, number of phagocytic bacteria, Hb, PCV, MCV, MCH, MCHC, number of RBC and WBC were studied. Treatments recorded enhancement in immune parameters compared to the control group. Significantly higher serum lysozyme level and phagocytic germs were detected in the groups fed black seed, ginger and cone flower (P&lt;0.05). However, no significant difference was found in RBC, Hb, PCV, MCV, MCH and MCHC of fish fed the diets containing essential oils and the control group (P&gt;0.05). The results of this study demonstrated that essential oils might strengthen the non-specific immunity of rainbow trout.
DOI: 10.6084/m9.figshare.6207911
title: Impacts of Breeder Age, Storage Time and Setter Ventilation Program on Incubation and Post-Hatch Performance of Broilers

description: ABSTRACT The impacts of breeder age (32 and 55 weeks), egg storage time (2-12 days), setter ventilation program (control-test) on incubation and post-hatch performance of broilers were investigated in this study. Young (Y) and old (O) breeders’ hatching eggs were incubated in two different setters operated by two different ventilation programs as control (C) and test (T). Incubation took place after a short (S) and long (L) time of storage in this study. According to the trial design, eight treatment groups were as YSC, YST, YLC, YLT, OSC, OST, OLC and OLT. In total 9600 eggs and 6400 chicks were used. Early stage embryonic mortality (ED) rates were lower and accordingly hatchability of fertile eggs (HF) were higher (p&lt;0.05) in YS than the other treatment groups. In contrast, incubation performance in eggs hatched in C and T programs were found to be similar. However, interaction between treatments were significant (p&lt;0.05) and ED and HF were significantly (p&lt;0.05) improved at OC. Liveability in growing period was affected (p&lt;0.05) by breeder age but it wasn’t affected by storage time and incubation program. Higher post-hatch performance was achieved in chicks of OST but differences between groups weren’t significant except for chick weights in comparison by breeder age. However, interactions between treatments were significant (p&lt;0.05) and reached the highest post-hatch performance in YST (p&lt;0.05). In conclusion, the long time storage of old breeders’ eggs improved incubation and post-hatch performance when they were incubated at test program.
DOI: 10.6084/m9.figshare.6207911.v1
title: Impacts of Breeder Age, Storage Time and Setter Ventilation Program on Incubation and Post-Hatch Performance of Broilers

description: ABSTRACT The impacts of breeder age (32 and 55 weeks), egg storage time (2-12 days), setter ventilation program (control-test) on incubation and post-hatch performance of broilers were investigated in this study. Young (Y) and old (O) breeders’ hatching eggs were incubated in two different setters operated by two different ventilation programs as control (C) and test (T). Incubation took place after a short (S) and long (L) time of storage in this study. According to the trial design, eight treatment groups were as YSC, YST, YLC, YLT, OSC, OST, OLC and OLT. In total 9600 eggs and 6400 chicks were used. Early stage embryonic mortality (ED) rates were lower and accordingly hatchability of fertile eggs (HF) were higher (p&lt;0.05) in YS than the other treatment groups. In contrast, incubation performance in eggs hatched in C and T programs were found to be similar. However, interaction between treatments were significant (p&lt;0.05) and ED and HF were significantly (p&lt;0.05) improved at OC. Liveability in growing period was affected (p&lt;0.05) by breeder age but it wasn’t affected by storage time and incubation program. Higher post-hatch performance was achieved in chicks of OST but differences between groups weren’t significant except for chick weights in comparison by breeder age. However, interactions between treatments were significant (p&lt;0.05) and reached the highest post-hatch performance in YST (p&lt;0.05). In conclusion, the long time storage of old breeders’ eggs improved incubation and post-hatch performance when they were incubated at test program.
DOI: 10.6084/m9.figshare.6207908
title: Selection of Coffea canephora parents from the botanical varieties Conilon and Robusta for the production of intervarietal hybrids

description: ABSTRACT: The objective of this research was to quantify the genetic divergence of potential Coffea canephora parents, with the goal of developing progenies that associate the best traits of the Conilon and Robusta botanical varieties for hybrid vigor expression. Thus, 10 morphological and productive characteristics of 130 clones of Conilon and Robusta botanical varieties and their intervarietal hybrids were evaluated over 2 years. The experiment was conducted in a randomized block design with four replicates of four plants per plot. For selection of parents, the main component analysis was used to associate values with reference points obtained from the average of each botanical variety. The first two principal components allowed for the separation of the botanical varieties representing the variability contained in the original data with 76% for the first year and 69% for the second year. Although, the genotype × years interaction had significant effects, there were minor differences in the grouping from one year to the next, which is associated with the higher repeatability estimates observed in this study. It was observed that crosses with the 16-1-81I, 9-1-82L, and 13-1-61I parents of the botanical variety Robusta and the 167I, 890E, and 130I parents of the Conilon botanical variety presented greater potential for obtaining selection gains.
DOI: 10.6084/m9.figshare.6207908.v1
title: Selection of Coffea canephora parents from the botanical varieties Conilon and Robusta for the production of intervarietal hybrids

description: ABSTRACT: The objective of this research was to quantify the genetic divergence of potential Coffea canephora parents, with the goal of developing progenies that associate the best traits of the Conilon and Robusta botanical varieties for hybrid vigor expression. Thus, 10 morphological and productive characteristics of 130 clones of Conilon and Robusta botanical varieties and their intervarietal hybrids were evaluated over 2 years. The experiment was conducted in a randomized block design with four replicates of four plants per plot. For selection of parents, the main component analysis was used to associate values with reference points obtained from the average of each botanical variety. The first two principal components allowed for the separation of the botanical varieties representing the variability contained in the original data with 76% for the first year and 69% for the second year. Although, the genotype × years interaction had significant effects, there were minor differences in the grouping from one year to the next, which is associated with the higher repeatability estimates observed in this study. It was observed that crosses with the 16-1-81I, 9-1-82L, and 13-1-61I parents of the botanical variety Robusta and the 167I, 890E, and 130I parents of the Conilon botanical variety presented greater potential for obtaining selection gains.
DOI: 10.6084/m9.figshare.6207905
title: CO2 Fluxes in Oil Palm Plantation in Eastern Amazonia

description: Abstract Terrestrial ecosystems are important for the CO2 exchange between surface and atmosphere. This work presents the atmospheric conditions and CO2 measurements in interspecific hybrid of oil palm in eastern Amazonia, during El Niño year. The experiment was carried out in Moju - Pará, where a micrometeorological tower was installed to obtain meteorological and CO2 data during the period from january to december 2015. The diurnal averages of CO2 uptake peaked at noon, with 22.3 (± 0.98) μmol m-2 s-1 in the wet season and 21.0 (± 0.47) μmol m-2 s-1 in the dry season. There was little variation in nocturnal averages of CO2 emission, about 5 (± 0.20) μmol m-2 s-1, in both wet and dry seasons. The diurnal averages of CO2 concentration were lower and nocturnal averages were higher. Generally, the daily CO2 exchange cycle presented a difference between the wet and dry season. These results contribute to a better understanding of the temporal trend of CO2 fluxes in agricultural crop during drought year in eastern Amazonia, generating new pieces of information about the interaction between oil palm and atmosphere.
DOI: 10.6084/m9.figshare.6207905.v1
title: CO2 Fluxes in Oil Palm Plantation in Eastern Amazonia

description: Abstract Terrestrial ecosystems are important for the CO2 exchange between surface and atmosphere. This work presents the atmospheric conditions and CO2 measurements in interspecific hybrid of oil palm in eastern Amazonia, during El Niño year. The experiment was carried out in Moju - Pará, where a micrometeorological tower was installed to obtain meteorological and CO2 data during the period from january to december 2015. The diurnal averages of CO2 uptake peaked at noon, with 22.3 (± 0.98) μmol m-2 s-1 in the wet season and 21.0 (± 0.47) μmol m-2 s-1 in the dry season. There was little variation in nocturnal averages of CO2 emission, about 5 (± 0.20) μmol m-2 s-1, in both wet and dry seasons. The diurnal averages of CO2 concentration were lower and nocturnal averages were higher. Generally, the daily CO2 exchange cycle presented a difference between the wet and dry season. These results contribute to a better understanding of the temporal trend of CO2 fluxes in agricultural crop during drought year in eastern Amazonia, generating new pieces of information about the interaction between oil palm and atmosphere.
DOI: 10.6084/m9.figshare.6207902
title: Case Study of a Cold air Outbreak Incursion Extreme Event in July 2013 on Brazilian Amazon Basin

description: Abstract The Amazon basin is located in the tropical region whose main characteristics are the high values of temperature and humidity of the air. However, cold and dry air incursions also penetrate the region, as occurred in July 2013. This fourth cold wave that affected the South American continent caused snow in cities of the South region, characterizing an intense polar mass with displacement to the North. The present work analyzed the behavior of meteorological variables in order to investigate the intensity of this effect. The dataset used were observations and reanalyses. The negative anomaly of the maximum temperature in Rio Branco was 15.9 °C, in Manicoré of 6.7 °C and Manaus of 2,0 °C. In this event, the most expressive values occurred at the maximum temperature. Also worth mentioning is a monthly contribution in precipitation rates, whose values were higher than 13.0 mm.day-1. Thus, it is concluded that the event of July 2013 was a case of intense cold, due to the fact that it moved to the central region of Amazonia and caused abrupt drops, mainly in maximum temperature, favoring rainfall in the tropical regions, as well as changes in surface pressure, humidity and wind.
DOI: 10.6084/m9.figshare.6207902.v1
title: Case Study of a Cold air Outbreak Incursion Extreme Event in July 2013 on Brazilian Amazon Basin

description: Abstract The Amazon basin is located in the tropical region whose main characteristics are the high values of temperature and humidity of the air. However, cold and dry air incursions also penetrate the region, as occurred in July 2013. This fourth cold wave that affected the South American continent caused snow in cities of the South region, characterizing an intense polar mass with displacement to the North. The present work analyzed the behavior of meteorological variables in order to investigate the intensity of this effect. The dataset used were observations and reanalyses. The negative anomaly of the maximum temperature in Rio Branco was 15.9 °C, in Manicoré of 6.7 °C and Manaus of 2,0 °C. In this event, the most expressive values occurred at the maximum temperature. Also worth mentioning is a monthly contribution in precipitation rates, whose values were higher than 13.0 mm.day-1. Thus, it is concluded that the event of July 2013 was a case of intense cold, due to the fact that it moved to the central region of Amazonia and caused abrupt drops, mainly in maximum temperature, favoring rainfall in the tropical regions, as well as changes in surface pressure, humidity and wind.
DOI: 10.6084/m9.figshare.6207899
title: TRANSLATION: A MIRROR OF CHANGE? MAFALDA ANSWERS

description: ABSTRACT Many linguistic changes that have been taking place in Brazilian Portuguese have given rise to a growing gap between this language and European Portuguese. One of these changes is the near impossibility, in Brazilian Portuguese, of dropping the subject, due to the simplification of verbal morphology ascribable to the growing use of você(s) and a gente. As a result, Brazilian Portuguese takes distance from the group of languages which permit subject-dropping, like Spanish, Italian, and European Portuguese, and comes nearer to languages where the expression of the subject is mandatory, like French. Here we compare the grammars of Brazilian and European Portuguese using as corpus translations of strips from Quino's Mafalda made both in Brazil and Portugal. Once comic strips are a genre in which there is an ambition of approaching the reality of spoken language, we expect that those translations mirror the normal uses of oral interaction in each of the two language cultures where the translations have been made, despite of being a fake orality.
DOI: 10.6084/m9.figshare.6207899.v1
title: TRANSLATION: A MIRROR OF CHANGE? MAFALDA ANSWERS

description: ABSTRACT Many linguistic changes that have been taking place in Brazilian Portuguese have given rise to a growing gap between this language and European Portuguese. One of these changes is the near impossibility, in Brazilian Portuguese, of dropping the subject, due to the simplification of verbal morphology ascribable to the growing use of você(s) and a gente. As a result, Brazilian Portuguese takes distance from the group of languages which permit subject-dropping, like Spanish, Italian, and European Portuguese, and comes nearer to languages where the expression of the subject is mandatory, like French. Here we compare the grammars of Brazilian and European Portuguese using as corpus translations of strips from Quino's Mafalda made both in Brazil and Portugal. Once comic strips are a genre in which there is an ambition of approaching the reality of spoken language, we expect that those translations mirror the normal uses of oral interaction in each of the two language cultures where the translations have been made, despite of being a fake orality.
DOI: 10.6084/m9.figshare.6207893
title: EXTRACTION OF R-(+)-LIMONENE FROM ORANGE PEELS: ASSESSEMENT AND OTIMIZATION OF THE GREENNESS OF TRADITIONAL EXTRACTION PROCESSES

description: This article reports a study, performed in educational context, to increase the greenness of the extraction of R-(+)-limonene from orange peels. Protocols from the literature were evaluated with the green star (GS)metric and new protocols, based in them, were designed to increase the overall greenness. These were tested in the laboratory and their greenness was assessed by traditional greenness metrics and metrics inspired in the industrial activity. Results showed that the steam distillation technique presents the largest overall greenness when evaluated by the GS, because organic solvents are not involved, but mass and energy metrics indicate that the extractions by solvent techniques are greener. This conflict denotes the complexity of the concept of chemical greenness and the need of using several types of metrics when greenness evaluation is at stake. The results show that the inclusion of experiments on the R-(+)-limonene extraction in the educational laboratory is efficient to show the difficulties of assessing the greenness of chemistry and of implementing Green Chemistry.
DOI: 10.6084/m9.figshare.6207893.v1
title: EXTRACTION OF R-(+)-LIMONENE FROM ORANGE PEELS: ASSESSEMENT AND OTIMIZATION OF THE GREENNESS OF TRADITIONAL EXTRACTION PROCESSES

description: This article reports a study, performed in educational context, to increase the greenness of the extraction of R-(+)-limonene from orange peels. Protocols from the literature were evaluated with the green star (GS)metric and new protocols, based in them, were designed to increase the overall greenness. These were tested in the laboratory and their greenness was assessed by traditional greenness metrics and metrics inspired in the industrial activity. Results showed that the steam distillation technique presents the largest overall greenness when evaluated by the GS, because organic solvents are not involved, but mass and energy metrics indicate that the extractions by solvent techniques are greener. This conflict denotes the complexity of the concept of chemical greenness and the need of using several types of metrics when greenness evaluation is at stake. The results show that the inclusion of experiments on the R-(+)-limonene extraction in the educational laboratory is efficient to show the difficulties of assessing the greenness of chemistry and of implementing Green Chemistry.
DOI: 10.6084/m9.figshare.6207890
title: Equine mediastinal lymphoma - case report

description: ABSTRACT A 20-year-old mixed breed mare was referred to the veterinary hospital with history of weight loss, loss of appetite, and swelling of the chest and neck. On physical exam the patient was in tachycardia and tachypnea and laboratory results indicated hyperproteinemia, hyperfibrinogenemia and hypoalbuminemia. On cardiac auscultation, the pulmonary and aortic valves sounds were not audible. Presence of pleural effusion and heart caudal displacement were identified on electrocardiographic and Doppler echocardiography evaluation and a mass at the base of the heart was oberved through ultrasound scanning around the heart. To alleviate the respiratory condition, thoracic drainage was performed. The pleural effusion presented characteristic serosanguineous with cytologic inflammatory. After returning to stall, the animal died. In necropsy, the presence of a mass positioned around the brachiocephalic trunk was identified and histopathology results were compatible with diffuse small and medium cell lymphoma.
DOI: 10.6084/m9.figshare.6207890.v1
title: Equine mediastinal lymphoma - case report

description: ABSTRACT A 20-year-old mixed breed mare was referred to the veterinary hospital with history of weight loss, loss of appetite, and swelling of the chest and neck. On physical exam the patient was in tachycardia and tachypnea and laboratory results indicated hyperproteinemia, hyperfibrinogenemia and hypoalbuminemia. On cardiac auscultation, the pulmonary and aortic valves sounds were not audible. Presence of pleural effusion and heart caudal displacement were identified on electrocardiographic and Doppler echocardiography evaluation and a mass at the base of the heart was oberved through ultrasound scanning around the heart. To alleviate the respiratory condition, thoracic drainage was performed. The pleural effusion presented characteristic serosanguineous with cytologic inflammatory. After returning to stall, the animal died. In necropsy, the presence of a mass positioned around the brachiocephalic trunk was identified and histopathology results were compatible with diffuse small and medium cell lymphoma.
DOI: 10.6084/m9.figshare.6207887
title: Placental osseous metaplasia associated with abortion in a mare: case report

description: ABSTRACT The aim of this report is to describe a case of marked osseous metaplasia in the placenta associated with abortion in a mare. A 19-year-old Campolina mare with a history of multiple parturitions and absence of reproductive failure, aborted a fetus with approximately six months of gestation. There were no gross changes in the fetus and the placenta. Microscopically, there was multifocal replacement of fetal connective mesenchyme of the chorionic villi with well differentiated and partially mineralized osseous tissue (confirmed by von Kossa staining). To further characterize the collagenous component of the lesion, sections of the placenta were stained with Masson’s trichrome and Picrosirius red. Osseous tissue deposition had abundance of type I collagen. To the best of our knowledge, this is the first report of osseous metaplasia in the placenta of a mare associated with abortion at mid gestation, presumably due to placentary insufficiency.