DOI: 10.25387/g3.29897192.v1
title: Supplemental Material for Tan et al., 2025

description:   
<strong>Video S1</strong> Dynamic fluorescence intensities change in intestinal granules of a dauer stage <em>C. elegans</em> larva, pseudo-colored-heat map. Snapshots of this video are shown in Figure 1 (pseudo-colored-green). Gut granule green flash events can be seen starting at about 0:00:20 in this video. Values represented are seconds and milliseconds and start (from 0) with the initiation of live imaging. The number on the vertical bar indicates the range of the color assignment of the heat map; Scale bar: 10 µm.
<strong>Video S2</strong> Dynamic fluorescence intensities change in intestinal granules of an L4 stage <em>C. elegans</em>. Snapshots of this video are shown in Figure 1. Gut granule green flash events can be seen starting at about 0:01:20 in this video. Scale bar: 10 µm.
<strong>Video S3</strong> Dynamic fluorescence intensities change in intestinal granules of an L4 stage <em>C. elegans</em>. The video was taken with a scanning confocal microscope in the <em>ex-vivo</em> setting. 
<strong>Video S4 </strong>Continuous exposure to DAPI makes the whole worm/ intestine autofluorescent. Values represented are seconds and milliseconds and start (from 0) with the initiation of live imaging.
<strong>Video S5 </strong>Birefringent granules in the intestine of adult <em>P. redivivus </em>are tightly associated with larger non-birefringent granules. The granules always stayed together. Values represented are seconds and milliseconds and start (from 0) with the initiation of live imaging. Scale bar: 50µm.
<strong>Video S6 </strong>Gut granule green flash was not often observed in the posterior end of the nematode intestines. The video is of the posterior end of an adult <em>O. tipulae</em> intestine, the same animal and region shown in Figure 6B. Values represented are seconds and milliseconds and start (from 0) with the initiation of live imaging. 
<strong>Video S7 </strong>Gut granule green flash in the anterior intestine of an adult hermaphrodite <em>O. tipulae</em>. The phenomenon is most commonly observed in the first intestinal cell ring. Snapshots of this video are shown in Figure 6D-E. Values represented are seconds and milliseconds and start (from 0) with the initiation of live imaging. Scale bar: 50µm.
<strong>Video S8 </strong>Gut granule green flash in the anterior intestine of a J4 hermaphrodite <em>P. pacificus</em>. Snapshots of a section of this video are shown in Figure 6G. Values represented are seconds and milliseconds and start (from 0) with the initiation of live imaging. Scale bar: 20µm.
<strong>Video S9 </strong>Gut granule green flash in the anterior intestine of a J4 hermaphrodite <em>S. hermaphroditum</em>. Snapshots of a section of this video are shown in Figure 6J-M. Scale bar: 10µm.
<strong>Video S10 </strong>Gut granule green flash in the anterior intestine of an L4 hermaphrodite <em>O. tipulae</em>. The phenomenon is most commonly observed in the first intestinal cell ring. The green flash seems to contribute to the build-up of overall cell autofluorescence. Snapshots of this video are shown in Figure S2A-B. Values represented are seconds and milliseconds, and start (from 0) with the initiation of live imaging. Scale bar: 10µm.
<strong>Video S11</strong> Dynamic fluorescence intensities change in intestinal granules of a J1 stage <em>S. hermaphroditum</em>. Snapshots of this video are shown in Figure S2C-E. Gut granule green flash events can be seen starting at about 0:00:30 in this video.