cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j02n516m
title: CIL:36673, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: Quick-freeze deep-etch image of a cytosolic region where trichocysts are developing. Vesicles, even small ones, contain contents that are compacting into paracrystalline form. These vesicles seem to have an affinity for each other as many are in contact. E-faces of the trichocyst membrane are pitted to varying extents. The fracture plane reveals different planes of packing in the core. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 15,900X. Bar = 0.5µm.
DOI: 10.6075/j07s7mq2
title: CIL:36672, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: In the case of a developing organelle, once the vesicles have grown to 1-2µm in diameter an electron-opaque crystal begins to grow at the center of the developing trichocyst. The paracrystalline material is finely striated. TEM taken on 12/9/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 28,599X. Bar = 0.2µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0qj7g72
title: CIL:36653, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: The micronuclear separation spindle. Nuclear pores are not empty perforations but are closed by nonetchable material. The separation spindle must elongate as microtubules lengthen and/or slide against each other as they grow. TEM taken on 5/12/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm.
description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
DOI: 10.6075/j0x9297j
title: CIL:36650, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Except that the nuclear envelope remains intact, micronuclear mitosis occurs in a normal way passing through the four stages of prophase, metaphase, anaphase and telophase. In this example a micronucleus was sectioned in early anaphase and shows condensed daughter chromosomes at each pole. Chromosomes are separated by a large bundle of microtubules making up an intranuclear spindle. The separation spindle will become a very long thin tube (see Fig.20 in Allen, Ueno and Fok, J. Protozool. 35:400-407, 1988). The image shown here has very low contrast because the cell was taken from a culture in which a 15 minute treatment in ammonium chloride was used to see what effect this base would have on its acidic compartments. The cell was then incubated in diaminobenzidine. As usual the cristae of mitochondria become electron-opaque following this treatment. TEM taken on 6/11/86 by R. Allen with Zeiss 10A operating at 80kV. Neg. 4,000X. Bar = 1µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0vm4b76
title: CIL:36647, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Grazing thin section through the nuclear envelope of a macronucleus. Nuclear pores are packed so tightly that they often form a hexagonal array with 6 pores surrounding a central pore. The pores often have electron opaque dots in their centers which indicate passage of ribonucleoprotein particles (RNP) between the nucleus and the cytoplasm. TEM taken on 1/4/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 17,500X. Bar = 0.2µm.
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0416w02
title: CIL:36644, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Large patches of rough ER surrounded by Golgi stacks (visible only at high magnification) have formed in the cytosol along with fields of lipid bodies. By day 2 these ER and lipid fields will be dispersed or depleted. For culture age study see Fok and Allen, Eur. J. Cell Biol. 25:182-193, 1981. TEM taken on 4/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 4,050X. Bar = 1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0ww7gj3
title: CIL:36666, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: A relatively low magnification view of the anterior end of Paramecium showing the placement and size of trichocysts relative to the cortex and cytoplasmic organelles. Trichocysts docked at the crests of the surface ridges have ‚Äúcarrot‚Äù shaped bodies surmounted by a spike that is long enough to penetrate from below the infraciliary lattice all the way to the tip of the ridge. Two trichocysts have been internally discharged into elongated shafts during fixation. cv, contractile vacuole. TEM taken on 12/2/83 by R. Allen with Zeiss 10A operating at 80kV. Neg. 1,600X. Bar = 2µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0fn155q
title: CIL:36658, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: In an enlargement of a pronucleus in the chromatin material appears as sparsely scattered opaque bodies, as may be expected of a haploid nucleus, in a medium dense nucleoplasm. The nuclear envelope of the pronucleus seems to be in very close contact with the nuclear envelope of the macronucleus as though pores might link and allow direct communication between the two nuclei. Remnants of microtubules are scattered in the nucleoplasm. TEM taken on 3/4/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0gq6wrx
title: CIL:36657, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: During self conjugation two cells from a 6-day old culture come together, bridges are formed between them and haploid products of meiotic division of their micronuclei are exchanged between the mating pairs. Two micronuclei are seen here which appear similar to the haploid pronucleus in the paper of Inaba et al. (Proc. Japan Acad. 42:394-398, 1966) which is passing through such a bridge in P. multimicronucleatum. In this micrograph two rounded micronuclei are lying very near the macronucleus. These may be the migratory and stationary pronuclei that will form the diploid synkaryon nucleus from which the new micronuclei and macronucleus will form after further mitotic divisions. Interestingly one of these nuclei appears to be in close contact with the macronucleus. TEM taken on 3/4/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0j1023x
title: CIL:36655, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Conjugating cells come together during or following meiosis and exchange promicronuclei. The cells actually fuse together at ridge tips and openings are formed between the two cells. TEM taken on 6/6/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j04m93fc
title: CIL:36638, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Peroxisomes are sometimes associated with lipid bodies as they are in this example. This cell was pretreated with 4% DMSO which increases the permeability of its membranes. Thus the peroxisomes are strongly stained following hydrogen peroxide and diaminobenzidine incubation. TEM taken on 4/7/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm.
description: This cell was pretreated with 4% DMSO which increases the permeability of its membranes. Thus the peroxisomes are strongly stained following hydrogen peroxide and diaminobenzidine incubation. Then standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
DOI: 10.6075/j0xd10bt
title: CIL:36633, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Enlargement of two mitochondria showing the cross sections of tubular cristae bear one pair of protrusions (these are the F1 subunits of F1F0 ATP synthase enzymes) while rows of protrusions can be viewed along some tangentially sectioned tubules. The tubules in some cases can be seen to arise from the inner membrane. TEM taken on 3/7/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X. Bar = 0.5nm. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40501) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0ms3rp5
title: CIL:36631, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Occasionally mitochondria of Paramecium become filled with cristae that display a wavy or helical pattern. In thin sections these cristae are viewed as curved arcs as well as cross sectioned rows in different parts of the organelle. Stacks of decorated tubules of the contractile vacuole system are also seen in this micrograph. Cristae of mitochondria bear F1F0 ATP synthases while the decorated tubules bear the related V1V0 V-ATPase proton pump. The helical arrayed F1F0 and V1V0 units may be responsible for forming the tubular nature of the cristae and the tubular decorated spongiome (for discussion of this hypothesis see Allen, Protoplasma 189:1-8, 1995). TEM taken on 5/6/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,250X. Bar = 0.5µm.
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j05d8qs5
title: CIL:36648, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed
description: A freeze-fractured view of the nuclear envelope. The fracture in the middle of the figure exposes the cytosolic facing membrane. This can be distinguished by a fractured cytoplasmic organelle at the top of the picture. The lower part of the picture shows the nucleoplasm-facing membrane of the envelope. The nuclear pores penetrate through both membranes. It is not clear if the membranes have actually fractured along their hydrophobic centers or if the true surfaces of these two membranes are being viewed. TEM taken on 2/16/76 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,579X. Bar = 0.25µm.
DOI: 10.15468/dl.m23jz4
title: Occurrence Download

description: A dataset containing 270955 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((73.71697 35.36278,72.93395 31.77556,68.10537 25.36259,68.23587 20.9241,71.49843 19.82308,77.50154 7.16185,80.37259 11.02694,87.02821 19.70026,91.5958 20.31339,98.12092 27.92841,96.81589 30.32215,82.59113 28.96111,79.06757 36.1043,73.71697 35.36278))",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Anseriformes",
 "Year 1972-2017"
 ]
}

 The dataset includes 270955 records from 16 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009416-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.