DOI: 10.5061/dryad.p2ngf1vx9
title: Plant diversity and soil legacy independently affect the plant metabolome and induced responses following herbivory

description: Plant and soil biodiversity can have significant effects on herbivore
 resistance mediated by plant metabolites. Here, we disentangled the
 independent effects of plant diversity and soil legacy on constitutive and
 herbivore-induced plant metabolomes of three plant species in two
 complementary microcosm experiments. First, we grew plants in sterile soil
 with three different plant diversity levels. Second, single plant species
 were grown on soil with different plant diversity-induced soil legacies.
 We infested a subset of all plants with Spodoptera exigua larvae,
 a generalist leaf-chewing herbivore, and assessed foliar and root
 metabolomes.  Neither plant diversity nor soil legacy had
 significant effects on overall foliar, root, or herbivore-induced
 metabolome composition. Herbivore-induced metabolomes, however, differed
 from those of control plants. We detected 139 significantly regulated
 metabolites by comparing plants grown in monocultures with conspecifics
 growing in plant or soil legacy mixtures. Moreover, plant-plant and
 plant-soil interactions regulated 141 metabolites in herbivore-induced
 plants.  Taken together, plant diversity and soil legacy
 independently alter the concentration and induction of plant metabolites,
 thus affecting the plant’s defensive capability. This is a first step
 towards disentangling plant and soil biodiversity effects on herbivore
 resistance, thereby improving our understanding of the mechanisms that
 govern ecosystem functioning.
description: # Plant diversity and soil legacy independently affect the plant
 metabolome and induced responses following herbivory. The data presented
 here covers plant individual and tissue-specific metabolite data as well
 as plant individual and tissue-specific biomass. For detailed information
 how the data was acquired, please see the associated manuscript in Ecology
 and Evolution. ## Description of the Data and file structure General
 information: n/a denotes not available information, e.g., a metabolite
 that was not detected in a particular sample, or an empty cell in a
 remarks column for there were no remarks to be made during measurement
 Dataset: Constitutive Metabolome\_Meta-Data\_PPI.csv Column name
 Description Composition Realized plant species community; Ger - Geranium
 pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
 Sample.species Plant species name Tissue Sampled plant tissue Pot\_Nr
 Running microcosm number Diversity Plant species richness of the microcosm
 Pos\_1 Plant species position in any given microcosm Pos\_2 Plant species
 position in any given microcosm Pos\_3 Plant species position in any given
 microcosm Pos\_4 Plant species position in any given microcosm Pos\_5
 Plant species position in any given microcosm Pos\_6 Plant species
 position in any given microcosm Pos\_7 Plant species position in any given
 microcosm Pos\_8 Plant species position in any given microcosm Pos\_9
 Plant species position in any given microcosm Pos\_10 Plant species
 position in any given microcosm Pos\_11 Plant species position in any
 given microcosm Pos\_12 Plant species position in any given microcosm Ger
 Number of planted Geranium individuals in any given microcosm Leu Number
 of planted Leucanthemum individuals in any given microcosm Ran Number of
 planted Ranunculus individuals in any given microcosm sample.date Sample
 date DWmg Dry weight in mg Remarks Remarks Dataset: Constitutive
 Metabolome\_Meta-Data\_PSI.csv Column name Description Composition
 Realized plant species community; Ger - Geranium pratense; Leu -
 Leucanthemum vulgare; Ran - Ranunculus acris Sample.species Plant species
 name Tissue Sampled plant tissue Pot\_Nr Running microcosm number
 Diversity Plant species richness of the microcosm Plot\_ID Plot number of
 the Trait-Based Experiment used for sampling the live soil inoculum
 sample.date Sample date DWmg Dry weight in mg Remarks Remarks Dataset:
 Herbivore-induced Metabolome\_Meta-Data\_PPI.csv Column name Description
 Composition Realized plant species community; Ger - Geranium pratense; Leu
 - Leucanthemum vulgare; Ran - Ranunculus acris Sample.species Plant
 species name Pot\_Nr Running microcosm number Treatment Indicator if the
 sampled plant was a control plant or a herbivore-induced plant Diversity
 Plant species richness of the microcosm Pos\_1 Plant species position in
 any given microcosm Pos\_2 Plant species position in any given microcosm
 Pos\_3 Plant species position in any given microcosm Pos\_4 Plant species
 position in any given microcosm Pos\_5 Plant species position in any given
 microcosm Pos\_6 Plant species position in any given microcosm Pos\_7
 Plant species position in any given microcosm Pos\_8 Plant species
 position in any given microcosm Pos\_9 Plant species position in any given
 microcosm Pos\_10 Plant species position in any given microcosm Pos\_11
 Plant species position in any given microcosm Pos\_12 Plant species
 position in any given microcosm Ger Number of planted Geranium individuals
 in any given microcosm Leu Number of planted Leucanthemum individuals in
 any given microcosm Ran Number of planted Ranunculus individuals in any
 given microcosm sample.date Sample date DWmg Dry weight in mg Remarks
 Remarks Dataset: Herbivore-induced Metabolome\_Meta-Data\_PSI.csv Column
 name Description Composition Realized plant species community; Ger -
 Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
 Sample.species Plant species name Pot\_Nr Running microcosm number
 Treatment Indicator if the sampled plant was a control plant or a
 herbivore-induced plant Diversity Plant species richness of the microcosm
 Plot\_ID Plot number of the Trait-Based Experiment used for sampling the
 live soil inoculum sample.date Sample date DWmg Dry weight in mg Remarks
 Remarks Datasets: Constitutive Metabolome species\_PPI files (e.g.,
 Constitutive Metabolome\_Ger\_pra\_PPI\_leaves.csv) Column name
 Description Sample.species Plant species name Composition Realized plant
 species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare;
 Ran - Ranunculus acris Tissue Sampled plant tissue Pot\_Nr Running
 microcosm number Diversity Plant species richness of the microcosm
 sample.date Sample date DWmg Dry weight in mg richness Number of extracted
 and measured metabolites shannon Shannon diversity of secondary
 metabolites simpson Simpson diversity of secondary metabolites ENS.shannon
 Shannon diversity of secondary metabolites expressed as effective number
 ENS.simpson Simpson diversity of secondary metabolites expressed as
 effective number *The remaining columns* Metabolite concentration
 (Intensity per mg dry weight); Column name first contains mass-to-charge
 ratio then retention time Datasets: Constitutive Metabolome species\_PSI
 files (e.g., Constitutive Metabolome\_Ger\_pra\_PSI\_leaves.csv) Column
 name Description Sample.species Plant species name Composition Realized
 plant species community; Ger - Geranium pratense; Leu - Leucanthemum
 vulgare; Ran - Ranunculus acris Tissue Sampled plant tissue Pot\_Nr
 Running microcosm number Diversity Plant species richness of the microcosm
 Plot\_ID Plot number of the Trait-Based Experiment used for sampling the
 live soil inoculum sample.date Sample date DWmg Dry weight in mg richness
 Number of extracted and measured metabolites shannon Shannon diversity of
 secondary metabolites simpson Simpson diversity of secondary metabolites
 ENS.shannon Shannon diversity of secondary metabolites expressed as
 effective number ENS.simpson Simpson diversity of secondary metabolites
 expressed as effective number *The remaining columns* Metabolite
 concentration (Intensity per mg dry weight); Column name first contains
 mass-to-charge ratio then retention time Datasets: Herbivore-induced
 Metabolome species\_PPI files (e.g., Herbivore-induced
 Metabolome\_Ger\_pra\_PPI.csv) Column name Description Sample.species
 Plant species name Composition Realized plant species community; Ger -
 Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
 Pot\_Nr Running microcosm number Treatment Indicator if the sampled plant
 was a control plant or a herbivore-induced plant Diversity Plant species
 richness of the microcosm sample.date Sample date DWmg Dry weight in mg
 richness Number of extracted and measured metabolites shannon Shannon
 diversity of secondary metabolites simpson Simpson diversity of secondary
 metabolites ENS.shannon Shannon diversity of secondary metabolites
 expressed as effective number ENS.simpson Simpson diversity of secondary
 metabolites expressed as effective number *The remaining columns*
 Metabolite concentration (Intensity per mg dry weight); Column name first
 contains mass-to-charge ratio then retention time Datasets:
 Herbivore-induced Metabolome species\_PSI files (e.g., Herbivore-induced
 Metabolome\_Ger\_pra\_PSI.csv) Column name Description Sample.species
 Plant species name Composition Realized plant species community; Ger -
 Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
 Pot\_Nr Running microcosm number Treatment Indicator if the sampled plant
 was a control plant or a herbivore-induced plant Diversity Plant species
 richness of the microcosm Plot\_ID Plot number of the Trait-Based
 Experiment used for sampling the live soil inoculum sample.date Sample
 date DWmg Dry weight in mg richness Number of extracted and measured
 metabolites shannon Shannon diversity of secondary metabolites simpson
 Simpson diversity of secondary metabolites ENS.shannon Shannon diversity
 of secondary metabolites expressed as effective number ENS.simpson Simpson
 diversity of secondary metabolites expressed as effective number *The
 remaining columns* Metabolite concentration (Intensity per mg dry weight);
 Column name first contains mass-to-charge ratio then retention time