cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/p2xn0-rdf91
title: wt_serum_blotfrombehind

description: This is a sample of wt grown in serum bottle that made nanowires.
description: <strong>Tilt Series Date:</strong> 2015-07-26</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Sample Preparation:</strong> The sample was put on the C side (glow discharged side) on the left. The blotting was done from behind on the rt side. Vitrobot was used. All other vitrobot conditions are the same.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-44/rawdata/</p>10045.mrc,  Tilt Series (Pixel Size 0.48 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-44/rawdata/10045.mrc"
        > <i class="download icon"></i> Download </a></p>

        10045_part61_3.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-26-44/3dimage_59640/10045_part61_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yg7hh-7pv32
title: U2OS-Tubulin Tracker Green

description: U2OS cells stained with tubulin tracker to locate midbodies. Cryo-FIB milling is used to make the sample thinner.
description: <strong>Tilt Series Date:</strong> 2017-06-13</p>
<strong>Data Taken By:</strong> Wei Zhao</p>
<strong>Species / Specimen:</strong> U2OS</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD-patch tracking</p>
<strong>Purification / Growth Conditions / Treatment:</strong> U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL.</p>
<strong>Sample Preparation:</strong> Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-06-13-5/rawdata/</p>TS4.mrc,  Tilt Series (Pixel Size 0.371 nm), 1.7 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        TS4_bin4_sirt_flipint.rec,  Reconstruction (Pixel Size 0.0 nm), 222.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-06-13-5/3dimage_85901/TS4_bin4_sirt_flipint.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/brjqs-wjm80
title: Coxiella burnetii_DeltaDotIcm_D5

description: Nine Mile phase II clone 4 (NMIIC4). Dot/Icm deletion strain, day-5 culture.
description: <strong>Tilt Series Date:</strong> 2017-01-14</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Refer to Heinzen lab</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-14-23/rawdata/</p>CbDeltaDot_day5m0010.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-14-23/rawdata/CbDeltaDot_day5m0010.mrc"
        > <i class="download icon"></i> Download </a></p>

        CbDeltaDot_day5m0010.rec,  Reconstruction (Pixel Size 1.56 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-14-23/3dimage_81139/CbDeltaDot_day5m0010.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yc6br-bhh91
title: #92+E.coli bamA purified

description: Raw data files of #92+E.coli bamA purified
description: <strong>Tilt Series Date:</strong> 2015-04-30</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Chris Hayes and David Low UCSB</p>
<strong>Sample Preparation:</strong> These are E.cloacae mini cells (#92) mixed with purified E.coli BamA protein receptor. Both cells and BamA were blocked with BSA- 100g/ml for 30 minutes before mixing for 20 minutes.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-30-7/rawdata/</p>PS96A-atlas0028.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-30-7/rawdata/PS96A-atlas0028.mrc"
        > <i class="download icon"></i> Download </a></p>

        PS96A-atlas0028_full.rec,  Reconstruction (Pixel Size 1.32 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-30-7/3dimage_55341/PS96A-atlas0028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3dt5x-kww27
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-282/rawdata/</p>T4SSWTNov25A025_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
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        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov25A025_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-282/3dimage_105032/T4SSWTNov25A025_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/608mb-n4s15
title: WT LP01 from Liu lab, Yale

description: Type four secretion system, T4SS
description: <strong>Tilt Series Date:</strong> 2016-11-28</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: -5.0 μm,  magnification: 15500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Data collected by Bo Hu in Jun Liu lab at Yale from their Polara. Partial WT strain data set. Primary literature:  doi: 10.1038/s41564-018-0165-z</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-328/rawdata/</p>T4SSWTNov26A035_driftcorr.mrc,  Tilt Series (Pixel Size 0.25 nm), 2.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-328/rawdata/T4SSWTNov26A035_driftcorr.mrc"
        > <i class="download icon"></i> Download </a></p>

        T4SSWTNov26A035_driftcorr.rec,  Reconstruction (Pixel Size 1.0 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2016-11-28-328/3dimage_105073/T4SSWTNov26A035_driftcorr.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/kfb9w-bhj98
title: Vibrio cholerae delta TcpB

description: Raw data files of Vibrio cholerae delta TcpB
description: <strong>Tilt Series Date:</strong> 2015-10-16</p>
<strong>Data Taken By:</strong> Andreas Kjaer</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grown ON in LB with ampicillin and IPTG, refreshed in LB shortly prior to freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-45/3dimage_73360/</p>10162015_TcpB_10046_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 7.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-45/3dimage_73360/10162015_TcpB_10046_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        10162015_TcpB_10046.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aka2015-10-16-45/rawdata/10162015_TcpB_10046.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/artqp-8kr58
title: Mycobacterium marinum

description: Grown on 2x LB to induce sporulation
description: <strong>Tilt Series Date:</strong> 2015-10-12</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Mycobacterium marinum</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Batchruntomo</p>
<strong>Collaborators and Roles:</strong> Will DePas from Dianne Newman's lab grew the cells</p>
<strong>Sample Preparation:</strong> Cells were frozen with Vitrobot Mark 4. Grids were positively glow discharged.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-12-7/3dimage_70184/</p>Mmarinum_oct20150007.rec,  Reconstruction (Pixel Size 1.92 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-12-7/3dimage_70184/Mmarinum_oct20150007.rec"
        > <i class="download icon"></i> Download </a></p>

        Mmarinum_oct20150007.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-10-12-7/rawdata/Mmarinum_oct20150007.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.pqak7y
title: Occurrence Download

description: A dataset containing 13133 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Hordeum taganrocense, Hordeum tanaiticum, Hordeum tetrastichum, Hordeum tetrastichum nudum, Hordeum transcaucasicum, Hordeum violaceum, Hordeum vulgare hexastichon, Hordeum vulgare abdulbasirovii, Hordeum vulgare antasiaticum, Hordeum vulgare bachteevii, Hordeum vulgare brachyurum, Hordeum vulgare chungense, Hordeum vulgare coeleste, Hordeum vulgare glabriviride, Hordeum vulgare hexastichon, Hordeum vulgare hexastichon, Hordeum vulgare hexastichum, Hordeum vulgare ismailii, Hordeum vulgare lukyanovae, Hordeum vulgare lvovii, Hordeum vulgare medioasiaticum, Hordeum vulgare multispiculum, Hordeum vulgare pallidum, Hordeum vulgare patimatae, Hordeum vulgare polystichon, Hordeum vulgare revelatum, Panicum dichotomiflorum, Leptoloma dichotomiflora, Panicum alternatum, Panicum ammophilum, Panicum ammophilum, Panicum amplectans, Panicum amplectens, Panicum aquaticum cartagoense, Panicum aquaticum chloroticum, Panicum aquaticum cartagoense, Panicum aquaticum chloroticum, Panicum brachiatum, Panicum brachiatum, Panicum chloroticum, Panicum chloroticum, Panicum chloroticum agreste, Panicum chloroticum agreste, Panicum chloroticum luxurians, Panicum chloroticum luxurians, Panicum chloroticum pingue, Panicum chloroticum sylvestre, Panicum chloroticum agreste, Panicum chloroticum luxurians, Panicum chloroticum luxurians)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen, Machine Observation)"
 ]
 }
 },
 "Continent is Europe",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2024"
 ]
}

 The dataset includes 13133 records from 352 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0047364-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/bhds5-0r931
title: nanowire+doublepili mutant+Chemo

description: This sample is batch 2 collection. This is from double pili mutant run on a chemostat.Batch 1 collection was from WT grown in serum bottle
description: <strong>Tilt Series Date:</strong> 2015-04-11</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -4.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh, USC</p>
<strong>Sample Preparation:</strong> Went to USC and froze using manual vitrobot. Only 1 grid was good among 6 and cells were isolated so couldn't detect any nanowires so far</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-11-3/rawdata/</p>1_atlas_nanowires0002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-11-3/rawdata/1_atlas_nanowires0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        1_atlas_nanowires0002_full.rec,  Reconstruction (Pixel Size 1.92 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-04-11-3/3dimage_53841/1_atlas_nanowires0002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1c0ks-gvs27
title: pED208_pilated

description: E coli strain with plasmid pED208 overexpresses T4SS and F-like pili
description: <strong>Tilt Series Date:</strong> 2015-01-14</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cells were obtained from Laura Frost</p>
<strong>Purification / Growth Conditions / Treatment:</strong> o/n liquid LB</p>
<strong>Sample Preparation:</strong> Vitrobot</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-40/rawdata/</p>pED208a0040.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-40/rawdata/pED208a0040.mrc"
        > <i class="download icon"></i> Download </a></p>

        pED208a0040_part121_6.rec,  Reconstruction (Pixel Size 2.0 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-40/3dimage_50656/pED208a0040_part121_6.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n3m7n-mzf17
title: P41+P41

description: Raw data files of P41+P41
description: <strong>Tilt Series Date:</strong> 2016-03-15</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Mycoplasma pneumoniae</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Edward Sheppard from Duncan Krause's lab prepared the cell culture</p>
  scy2016-03-15-6_slicer16740.jpg: attachment organelle scy2016-03-15-6_slicer16741.jpg: attachment organelle, fair view of the end
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-03-15-6/rawdata/</p>atlas10005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-03-15-6/rawdata/atlas10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10005_full.rec,  Reconstruction (Pixel Size 1.56 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2016-03-15-6/3dimage_73588/atlas10005_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3hks0-hfp29
title: Cos7 CHAMP4B-PAGFP VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2015-01-24</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells were grown on grids and transfected with LipofectamineSample Preparation:  manual blotting</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2015-01-24-4/rawdata/</p>15jan24_C205-1_CLEM_test34_AT1_BT2_0002.mrc,  Tilt Series (Pixel Size 0.5 nm), 3.4 GB  
        
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        15jan24_C205-1_CLEM_test34_AT1_BT2_0002_full.rec,  Reconstruction (Pixel Size 2.0 nm), 623.6 MB  
        
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