DOI: 10.34810/data1626
title: Replication Data for: "Differential biological effect of low doses of ionizing radiation depending on the radiosensitivity in a cell line model"

description: Exposure to low doses of ionizing radiation may be associated with an increased risk of cancer. However, there are studies that indicate that the development of cancer could be influenced by variation in individual radiosensitivity. The data in this file show the results of different biological parameters after irradiating two cell lines at 0, 20 and 500 mGy. These cell lines are described one as radioresistant and the other as radiosensitive.

The values ​​of the files correspond to: gene expression, DNA damage and its repair, as well as cell viability, cell proliferation and cell death
description: R Software, null
description: 1. Description of methods used for collection-generation of data: Data come from the analysis of two lymphoblastoid cell lines that were exposed to 0 , 20 and 500 mGy using 6 MV photon beams from a TrueBeam linear accelerator. Gene expression, DNA damage and its repair, cell viability, cell proliferation and cell death were studied. RNA extraction was done 24 h after irradiation using RNeasy Mini Kit. Differential expression analysis was done using limma removing unwanted variations with the Bioconductor R package sva. Double strand breaks were evaluated by means of γ-H2AX foci detection after 0, 2, 4 and 24 h post-irradiation. Cytogenetic analysis was done to detect chromosomme- and chromatide- type aberrations as well as gaps. Sister chromatide exchage was also evaluated. Cell proliferation was measured counting the number of metaphase spreads in their firts, second, or third cell division. Cell viability by the MTT assay was measured 24, 48 and 68 h post-irradiation, and cell death was evaluated with the Annexin-V-FLUOS Staining and assessed by flow cytometry. 2. Methods for processing the data: The file contains the data obtained for the two cell lines 20037 (radioresistant) and 4060 (radiosensitive). For RNA analysis limma package from R was used. To calculate the means and SEs of the γ-H2AX assay, cytogenetic analysis, cell proliferation, cell vialbility and cell death, basic R software was used. To measure cell death a specific program FlowJo was used. 3. Instrument- or software- specific information needed to interpret the data: CSV files can be opened in notepad or other text editors
R data file can be opened with the free Bioconductor package SummarizedExperiment (https://bioconductor.org/packages/release/bioc/html/SummarizedExperiment.html) using R software (https://www.r-project.org/). Data can be interpeted as explained in the following link https://www.bioconductor.org/packages/devel/bioc/vignettes/SummarizedExperiment/inst/doc/SummarizedExperiment.html. 4. Instruments, calibration and standards information: γ-H2AX and cytogenetic analysis were performed using an automated scanning fluorescence microscope system (Metafer 4, Meta Systems, Altlussheim, Germany). Cell death was measured by flow citometry with CytoFLEX (Beckman Coulter Life Science, Pasadena, CA, USA). Cell viability was evaluated using Sunrise absorbance microplate reader (TECAN, Männedorf, canton of Zürich, Switzerland), and RNA sequencing was done using the Illumina NextSeq 2000 with CytoFLEX (Beckman Coulter Life Science, Pasadena, CA, USA)