cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.90212.v1
title: Does extra CO2 improve phytoplankton growth in culture?

description: We noticed that the Pseudo-nitzschia delicatissima (strain LaPn-4) cultures were growing better than expected. We speculated that the culture’s growth suddenly improved because it was getting more CO2 than before. We reached this conclusion because: 1. The culture grew better as flasks were changed more frequently 2. The culture grew better as the flasks we kept them in increased in volume 3. The culture was in flasks and had to be poured to take fluorometer readings and to do dilutions To investigate this, we used three 100mL cultures in 250mL Erlenmeyer flasks. F-28 was aerated using a pasteur pipette attached with rubber tubing and airline hose to an aquarium pump. The volumes needed for measurements and dilutions were removed and added using a sterile pipette. F-27 was not aerated. In order to take measurements and dilute the appropriate volume was pipetted in and out of the flask. F-26 was not aerated either. In order to obtain culture for measurements and dilutions the appropriate volume was poured into and out of the flask. No pipettes were used. All flasks were shaken to suspend cells evenly before fluorescence measurements were taken. These flasks had to be diluted often both before and after these trials began. As a result, obtaining three points for calculating a growth rate was difficult. We calculated three different growth rates for each flask and took averages. Later, upon comparing the length of the cells in the same culture of the previous year, we noticed that the cells were significantly longer this year. This told us that the increased growth, health, and chain length was due to the culture undergoing sexual reproduction. The data we have shows that the aerated culture had the best average growth rate followed by the pipetted culture and finally the pour culture.
DOI: 10.6084/m9.figshare.90209
title: How many cells should we count?

description: These data were gathered in an effort to solve a problem with correlating microscopic cell counts and fluorescence measurements taken during the summer of 2001 from an oyster (Crassostrea virginica) grazing experiment reported in Thessen, A.E., Q. Dortch, T.M. Soniat and G.J. Doucette (2009) Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The cells in question are the diatom Pseudo-nitzschia. We wanted to answer the question, "Are we counting enough fields and cells to be representative of the sample?" We pulled several slides (slide # 707, 721, 723, 708) from the experiment of varying cell concentration and species, initially counted at different magnifications. We calculated mean and standard deviation for each field until the two values seemed to stabilize.
DOI: 10.6084/m9.figshare.90209.v1
title: How many cells should we count?

description: These data were gathered in an effort to solve a problem with correlating microscopic cell counts and fluorescence measurements taken during the summer of 2001 from an oyster (Crassostrea virginica) grazing experiment reported in Thessen, A.E., Q. Dortch, T.M. Soniat and G.J. Doucette (2009) Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The cells in question are the diatom Pseudo-nitzschia. We wanted to answer the question, "Are we counting enough fields and cells to be representative of the sample?" We pulled several slides (slide # 707, 721, 723, 708) from the experiment of varying cell concentration and species, initially counted at different magnifications. We calculated mean and standard deviation for each field until the two values seemed to stabilize.
DOI: 10.6084/m9.figshare.90208
title: How many cells should we count?

description: These data were gathered in an effort to solve a problem with correlating microscopic cell counts and fluorescence measurements taken during the summer of 2000 from an oyster (Crassostrea virginica) grazing experiment reported in Thessen, A.E., Q. Dortch, T.M. Soniat and G.J. Doucette (2009) Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The cells in question are the diatom Pseudo-nitzschia. We wanted to answer the question, "Are we counting enough fields and cells to be representative of the sample?" We pulled several slides (slide # 627, 633, 700, 688, 682 and 684) from the experiment of varying cell concentration and species, initially counted at different magnifications. We calculated mean and standard deviation for each field until the two values seemed to stabilize. These data did not solve the correlation problem. We later concluded that a fungal infection in the Pseudo-nitzschia culture had caused the problem.
DOI: 10.6084/m9.figshare.90206
title: Galaxy Zoo 1

description: The original Galaxy Zoo project ran from July 2007 until February 2009, before being replaced by Galaxy Zoo 2 and then, in turn by Galaxy Zoo: Hubble. Volunteers were asked to classify SDSS galaxies as belonging to one of six categories - elliptical, clockwise spiral, anticlockwise spiral, edge-on galaxies, 'star/don't know' or merger. These files contain the resulting classifications of nearly 900,000 galaxies drawn from the Sloan Digital Sky Survey. These files here are Zipped CSV files, gzip, FITS and VO formats available at http://data.galaxyzoo.org/ The project is described in Lintott et al., 2008, MNRAS, 389, 1179 (linked below) and the data release is described in Lintott et al. 2010. Anyone making use of the data should cite at least one of these papers in any resulting publications. FILES: Table 2 gives classifications of galaxies which have spectra included in SDSS Data Release 7. The fraction of the vote in each of the six categories is given, along with debiased votes in elliptical and spiral categories and flags identifying systems as classified as spiral, elliptical or uncertain. Table 3 gives classifications of galaxies included in the Galaxy Zoo sample which did not have spectra available in SDSS Data Release 7. It is not possible to estimate the bias in the sample, and so only the fraction of the vote in each of the six categories is given. Table 4 gives a series of measures of classification confidence. Tables 5 and 6 give the results from the bias study that introduced mirrored (table 5) and monochrome (table 6) images. Table 7 gives the fraction of votes in each of the six categories, combining results from the main and bias studies.
DOI: 10.6084/m9.figshare.90187.v1
title: roll144#21.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with an epifluorescence microscope using a 10X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered seawater. The oyster was 36mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation.
DOI: 10.6084/m9.figshare.90195.v2
title: The distribution pattern of WGA:GFP in the Drosophila larval brain upon its expression in the Bolwig's Organ

description: To express WGA:GFP robustly using the Gal4/UAS system, a Drosophila codon-optimized wheat germ agglutinin (WGA) was subcloned into the JFRC14-CS-dGFP construct (also called UAS-dWGA:GFP). This UAS-transgene was then integrated into a specific landing site, containing the 3xP3-RFP marker, in the 3rd chromosome. Flies containing the UAS-dWGA:GFP transgene were then crossed to a visual system targeting longGMR-Gal4 strain. (A) Progeny larvae from this cross displayed a punctate GFP-label in the Bolwig's Organ (larval visual system). (B) The GFP-label was quite widely distributed in the brain hemisphere outside of the Bolwig's Organ terminals (marked by RFP). Arrowheads indicate GFP-label in unidentified cells. These observation support a previous report that WGA can be transferred trans-synaptically in Drosophila (Tabuchi et al. 2000). Squiggle-arrow indicates a likely non-specific uptake area. The developing adult visual system zone is indicated by an asterisk.
DOI: 10.6084/m9.figshare.90186.v1
title: roll144#22.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with transmitted light using a 10X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered seawater. The oyster was 36mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation.
DOI: 10.6084/m9.figshare.90195.v3
title: The distribution pattern of WGA:GFP in the Drosophila larval brain upon its expression in the Bolwig

description: To express WGA:GFP robustly using the Gal4/UAS system, a Drosophila codon-optimized wheat germ agglutinin (WGA) was subcloned into the JFRC14-CS-dGFP construct (also called UAS-dWGA:GFP). This UAS-transgene was then integrated into a specific landing site, containing the 3xP3-RFP marker, in the 3rd chromosome. Flies containing the UAS-dWGA:GFP transgene were then crossed to a visual system targeting longGMR-Gal4 strain. (A) Progeny larvae from this cross displayed a punctate GFP-label in the Bolwig's Organ (larval visual system). (B) The GFP-label was quite widely distributed in the brain hemisphere outside of the Bolwig's Organ terminals (marked by RFP). Arrowheads indicate GFP-label in unidentified cells. These observation support a previous report that WGA can be transferred trans-synaptically in Drosophila (Tabuchi et al. 2000). Squiggle-arrow indicates a likely non-specific uptake area. The developing adult visual system zone is indicated by an asterisk.
DOI: 10.6084/m9.figshare.90185.v1
title: roll1473#9.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with transmitted light using a 40X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The oyster was 40 mm in length. The feces is from an oyster that was starved for one day in filtered seawater. It was stored in an ambient flow-through seawater system at LUMCON before starvation.
DOI: 10.6084/m9.figshare.90195.v4
title: Distribution pattern of WGA:GFP in the Drosophila larval brain upon expression in the Bolwig's Organ

description: To express WGA:GFP robustly using the Gal4/UAS system, a Drosophila codon-optimized wheat germ agglutinin (WGA) was subcloned into the JFRC14-CS-dGFP construct (also called UAS-dWGA:GFP). This UAS-transgene was then integrated into a specific landing site, containing the 3xP3-RFP marker, in the 3rd chromosome. Flies containing the UAS-dWGA:GFP transgene were then crossed to a visual system targeting longGMR-Gal4 strain. (A) Progeny larvae from this cross displayed a punctate GFP-label in the Bolwig's Organ (larval visual system). (B) The GFP-label was quite widely distributed in the brain hemisphere outside of the Bolwig's Organ terminals (marked by RFP). Arrowheads indicate GFP-label in unidentified cells. These observation support a previous report that WGA can be transferred trans-synaptically in Drosophila (Tabuchi et al. 2000). Squiggle-arrow indicates a likely non-specific uptake area. The developing adult visual system zone is indicated by an asterisk.
DOI: 10.6084/m9.figshare.90184.v1
title: roll144#13.jpg

description: This picture of oyster (Crassostrea virginica) pseudofeces was taken with an epifluorescence microscope using a 10X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was fed Pseudo-nitzschia delicatissima strain LaPn-4. The oyster was 36mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The LaPn-4 strain was isolated in Louisiana waters in 1999 and is characterized in Thessen et al. 2005 Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. J. Phyc. 41:21-29.
DOI: 10.6084/m9.figshare.90195.v5
title: Distribution pattern of WGA:GFP in the Drosophila larval brain upon expression in the Bolwig

description: To express WGA:GFP robustly using the Gal4/UAS system, a Drosophila codon-optimized wheat germ agglutinin (WGA) was subcloned into the JFRC14-CS-dGFP construct. This UAS-dWGA:GFP transgene was then integrated into a specific landing site, containing the 3xP3-RFP marker, in the 3rd chromosome. Flies containing the UAS-dWGA:GFP transgene were then crossed to a visual system targeting longGMR-Gal4 strain. (A) Progeny larvae from this cross displayed a punctate GFP-label in the Bolwig's Organ (larval visual system). (B) The GFP-label was quite widely distributed in the brain hemisphere outside of the Bolwig's Organ terminals (marked by RFP). Arrowheads indicate GFP-label in unidentified cells. These observation support a previous report that WGA can be transferred trans-synaptically in Drosophila (Tabuchi et al. 2000). Squiggle-arrow indicates a likely non-specific uptake area. The developing adult visual system zone is indicated by an asterisk.
DOI: 10.6084/m9.figshare.90183.v1
title: roll144#12.jpg

description: This picture of oyster (Crassostrea virginica) pseudofeces was taken with an epifluorescence microscope using a 10X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was fed Pseudo-nitzschia delicatissima strain LaPn-4. The oyster was 36mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The LaPn-4 strain was isolated in Louisiana waters in 1999 and is characterized in Thessen et al. 2005 Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. J. Phyc. 41:21-29.
DOI: 10.6084/m9.figshare.90195.v6
title: Distribution pattern of WGA:GFP in the Drosophila larval brain upon expression in the Bolwig's Organ

description: To express WGA:GFP robustly using the Gal4/UAS system, a Drosophila codon-optimized wheat germ agglutinin (WGA) was subcloned into the JFRC14-CS-dGFP construct. This UAS-dWGA:GFP transgene was then integrated into a specific landing site, containing the 3xP3-RFP marker, in the 3rd chromosome. Flies containing the UAS-dWGA:GFP transgene were then crossed to a visual system targeting longGMR-Gal4 strain. (A) Progeny larvae from this cross displayed a punctate GFP-label in the Bolwig's Organ (larval visual system). (B) The GFP-label was quite widely distributed in the brain hemisphere outside of the Bolwig's Organ terminals (marked by RFP). Arrowheads indicate GFP-label in unidentified cells. These observation support a previous report that WGA can be transferred trans-synaptically in Drosophila (Tabuchi et al. 2000). Squiggle-arrow indicates a likely non-specific uptake area. The developing adult visual system zone is indicated by an asterisk.
DOI: 10.6084/m9.figshare.90181.v1
title: roll144#10.jpg

description: This picture of oyster (Crassostrea virginica) excretia was taken with transmitted light. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011.
DOI: 10.6084/m9.figshare.90195.v7
title: Distribution pattern of WGA:GFP in the Drosophila larval brain upon expression in the Bolwig

description: To express WGA:GFP robustly using the Gal4/UAS system, a Drosophila codon-optimized wheat germ agglutinin (WGA) was subcloned into the JFRC14-CS-dGFP construct. This UAS-dWGA:GFP transgene was then integrated into a specific landing site, containing the 3xP3-RFP marker, in the 3rd chromosome. Flies containing the UAS-dWGA:GFP transgene were then crossed to a visual system targeting longGMR-Gal4 strain. (A) Progeny larvae from this cross displayed a punctate GFP-label in the Bolwig's Organ (larval visual system). (B) The GFP-label was quite widely distributed in the brain hemisphere outside of the Bolwig's Organ terminals (marked by RFP). Arrowheads indicate GFP-label in unidentified cells. These observations support a previous report that WGA can be transferred trans-synaptically in Drosophila (Tabuchi et al. 2000). Squiggle-arrow indicates a likely non-specific uptake area. The developing adult visual system zone is indicated by an asterisk.
DOI: 10.6084/m9.figshare.90178.v1
title: roll143#25.jpg

description: This picture of oyster (Crassostrea virginica) pseudofeces was taken with an epifluorescence microscope using a 40X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The pseudofeces is from an oyster that was fed Pseudo-nitzschia delicatissima strain LaPn-4. The sample was collected approximately one hour of the feeding. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The LaPn-4 strain was isolated in Louisiana waters in 1999 and is characterized in Thessen et al. 2005 Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. J. Phyc. 41:21-29.
DOI: 10.6084/m9.figshare.90177.v1
title: roll143#19.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with an epifluorescent microscope using a 100X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered sea water. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The image shows an intact centric diatom, probably a Ptychodiscus.
DOI: 10.6084/m9.figshare.90179.v1
title: roll143#26.jpg

description: This picture of oyster (Crassostrea virginica) pseudofeces was taken with transmitted light using a 40X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The pseudofeces is from an oyster that was fed Pseudo-nitzschia delicatissima strain LaPn-4. The sample was collected approximately one hour of the feeding. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The LaPn-4 strain was isolated in Louisiana waters in 1999 and is characterized in Thessen et al. 2005 Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. J. Phyc. 41:21-29.
DOI: 10.6084/m9.figshare.90175.v1
title: roll143#11.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with transmitted light using a 40X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered sea water. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation.
DOI: 10.6084/m9.figshare.90174.v1
title: roll143#16.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with transmitted light using a 100X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered sea water. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The image shows plant debris that was found in the feces.
DOI: 10.6084/m9.figshare.90176.v1
title: roll143#22.jpg

description: This picture of oyster (Crassostrea virginica) pseudofeces was taken with an epifluorescent microscope using a 20X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The pseudofeces is from an oyster that was fed Pseudo-nitzschia delicatissima strain LaPn-4. The sample was collected approximately one hour of the feeding. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The LaPn-4 strain was isolated in Louisiana waters in 1999 and is characterized in Thessen et al. 2005 Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. J. Phyc. 41:21-29.
DOI: 10.6084/m9.figshare.90173.v1
title: roll143#15.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with transmitted light using a 100X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered sea water. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation. The image shows plant debris that was found in the feces.
DOI: 10.6084/m9.figshare.90170.v1
title: roll143#6.jpg

description: This picture of oyster (Crassostrea virginica) feces was taken with an epifluorescent microscope using a 40X objective. The photo was taken on July 23, 2000 by Quay Dortch at LUMCON as a part of the experiment series published in Thessen et al. 2009 Crassostrea virginica grazing on toxic and non-toxic diatoms. Toxicon.doi:10.1016/j.toxicon.2009.10.011. The feces is from an oyster that was starved for one day in filtered sea water. The oyster was 40mm in length. It was obtained from an aquaculture facility, stored in an ambient flow-through seawater system at LUMCON before starvation and experimentation.