cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6991688.v1
title: Nopalea cactus pear fertilized with nitrogen: morphometric, productive and nutritional characteristics

description: ABSTRACT. This study aimed to evaluate the structural, productive and nutritional characteristics of cactus pear (Nopalea cochenillifera Salm Dyck. cv. Miúda/Doce) under five nitrogen doses and two planting orientations. A randomized block design in a 5 x 2 factorial scheme was used in the scheme: five nitrogen doses (0, 150, 300, 450 and 600 kg of N ha-1), and two planting orientations (North/South and East/West), with three replicates. Except for the thickness of the secondary (p = 0.04) and tertiary cladodes (p = 0.02), the morphometric characteristics of the cactus pear were not influenced by the nitrogen doses (p &gt; 0.05). The mean height was 120 cm, the areas of the primary, secondary and tertiary cladodium were 160.9; 208 and 158.4 cm², respectively. The application of nitrogen fertilizer, in the dry conditions and doses evaluated, did not affect the production of green matter (PGM, p = 0.56), dry matter (PDM, p = 0.74) and dry matter content (DM, p = 0.72), with averages of 276 t ha-1, 43.2 t ha-1 and 15.7%, respectively. The increase of the nitrogen doses does not improve the majority of the morphometric characteristics or the dry matter yield of the cactus pear cv. Miúda at 730 days after planting.
DOI: 10.6084/m9.figshare.6991679
title: Influence of proprioceptive neuromuscular facilitation on the muscle tonus and amplitude of movement in HTLV-1-infected patients with HAM/TSP

description: Abstract An HTLV-1-infected patient can develop paraparesis that limits their movements. Rehabilitation techniques could improve the motor abilities of these patients. The present study investigates five cases of physical therapy intervention in HTLV-1 patients to evaluate the influence of functional rehabilitation on the tonus and range of motion (ROM) of HTLV-1 patients with spasticity. The patients had a gain of ROM, especially in the lower limb, and reduction in hypertonia/spasticity after functional treatment. The reduction in hypertonia increased the ROM. Thus, functional methods may be valuable for the rehabilitation of HTLV-1 patients with neurological damage.
DOI: 10.6084/m9.figshare.6991679.v1
title: Influence of proprioceptive neuromuscular facilitation on the muscle tonus and amplitude of movement in HTLV-1-infected patients with HAM/TSP

description: Abstract An HTLV-1-infected patient can develop paraparesis that limits their movements. Rehabilitation techniques could improve the motor abilities of these patients. The present study investigates five cases of physical therapy intervention in HTLV-1 patients to evaluate the influence of functional rehabilitation on the tonus and range of motion (ROM) of HTLV-1 patients with spasticity. The patients had a gain of ROM, especially in the lower limb, and reduction in hypertonia/spasticity after functional treatment. The reduction in hypertonia increased the ROM. Thus, functional methods may be valuable for the rehabilitation of HTLV-1 patients with neurological damage.
DOI: 10.6084/m9.figshare.6991673
title: Effects of easy soluble carbohydrate and bacterial inoculant supplementations on silage quality of frosted maize

description: ABSTRACT. The present experiments were conducted to assess the effects of barley, Lactobacillus bacteria and lactic acid bacteria (LAB) and enzyme mixture supplementations on chemical composition and some quality parameters of naturally frosted maize silage. The treatments were as follows; T1: Control (no supplementation, C); T2: barley 20 g kg-1, B20; T3: barley 40 g kg-1, B40; T4: 1.5 g ton-1, a mixture of LAB; and T5: 2 g ton1, a mixture LAB+enzyme, LEN. Barley supplementation to frosted maize increased dry matter (DM) (p &lt; 0.01). The pH value was higher in LAB group (p &lt; 0.01). Treatments supplemented LAB and LEN significantly reduced crude protein contents (p &lt; 0.001). Lactic acid contents decreased in all treatments as compared to control group and ranged between 2.46 and 5.40% DM (p &lt; 0.05). The metabolisable energy content was significantly higher in B40 than in B20, LAB and LEN groups. Mean Fleig points showed with an “excellent” quality silage. In conclusion, supplementation of barley, LEN and inoculation of LAB to frosted maize had a good quality silage. The LEN supplementation may be used for efficient ensiling of frosted maize due to their ability to keep its quality in low DM crop maize silage.
DOI: 10.6084/m9.figshare.6991676
title: Watermelon cultivation in the semi-arid irrigated with different heights of treated domestic sewage

description: ABSTRACT The aim of this paper was to assess the agronomic viability of using anaerobically treated domestic sewage in cultivation of Crimson Sweet watermelon. In a field experiment conducted in the semi-arid region of Pernambuco state, Brazil, four treatments (T) were used to assess the effects of irrigation using dripping system on fruits and soil. Four heights of 50% (T1), 75% (T2), 100% (T3) and 125% (T4), based on the calculated evapotranspiration and irrigation needs, were applied. The treated effluent characteristics indicated compliance with the recommendations for agricultural application, but the concentration of suspended solids (114 mg.L-1) demanded frequent cleansing of the dripper blockages. We observed an increase in the cation exchange capacity and a decrease of the soil pH in all the treatments. The best results were obtained with the treatment T4 (height of 125%), which showed watermelons of bigger size (20.78 cm), as well as the highest productivity (61.1 t.ha-1) and fruit weight (7.12 kg.un-1). These values are higher than those of the country and Pernambuco state, demonstrating the agronomic feasibility of reuse under the studied conditions.
DOI: 10.6084/m9.figshare.6991676.v1
title: Watermelon cultivation in the semi-arid irrigated with different heights of treated domestic sewage

description: ABSTRACT The aim of this paper was to assess the agronomic viability of using anaerobically treated domestic sewage in cultivation of Crimson Sweet watermelon. In a field experiment conducted in the semi-arid region of Pernambuco state, Brazil, four treatments (T) were used to assess the effects of irrigation using dripping system on fruits and soil. Four heights of 50% (T1), 75% (T2), 100% (T3) and 125% (T4), based on the calculated evapotranspiration and irrigation needs, were applied. The treated effluent characteristics indicated compliance with the recommendations for agricultural application, but the concentration of suspended solids (114 mg.L-1) demanded frequent cleansing of the dripper blockages. We observed an increase in the cation exchange capacity and a decrease of the soil pH in all the treatments. The best results were obtained with the treatment T4 (height of 125%), which showed watermelons of bigger size (20.78 cm), as well as the highest productivity (61.1 t.ha-1) and fruit weight (7.12 kg.un-1). These values are higher than those of the country and Pernambuco state, demonstrating the agronomic feasibility of reuse under the studied conditions.
DOI: 10.6084/m9.figshare.6991670
title: Stability and adaptability for wood volume in the selection of Eucalyptus saligna in three environments

description: Abstract: The objective of this work was to evaluate the genotype x environment (GxE) interaction in open-pollinated Eucalyptus saligna progenies for simultaneous selection for greater stability and adaptability for wood volume, as well as to compare the selection strategies through combined and individual analyses in three environments. Three experiments were conducted in a randomized complete block design, with four replicates and six plants per plot, with 102 to 122 progenies. Three years after planting, survival rate and wood volume were evaluated. The genetic parameters were estimated by the maximum restricted likelihood and best linear unbiased prediction (REML/Blup) methods, and the stability and adaptability analysis was conducted using the harmonic mean of the relative performance of genetic values (HMRPGV). The highest survival rate (82%) was observed in Sabinópolis and the highest volume (120 m³ ha-1) in Lençóis Paulista. The mean heritability of progenies for the two analyzed variables was considered high, and the genetic correlations between survival rate and volume were weak for all sites. The G×E interaction was significant and of the complex type, with genotype correlation between sites of 47%, indicating that the ranking of the best progenies differed in each studied environment. The analysis of stability and adaptability indicated the possibility of selecting progenies with good performance in the three environments; however, to improve the quality of the evaluated characteristics, it is necessary to select specific progenies for each environment.
DOI: 10.6084/m9.figshare.6991673.v1
title: Effects of easy soluble carbohydrate and bacterial inoculant supplementations on silage quality of frosted maize

description: ABSTRACT. The present experiments were conducted to assess the effects of barley, Lactobacillus bacteria and lactic acid bacteria (LAB) and enzyme mixture supplementations on chemical composition and some quality parameters of naturally frosted maize silage. The treatments were as follows; T1: Control (no supplementation, C); T2: barley 20 g kg-1, B20; T3: barley 40 g kg-1, B40; T4: 1.5 g ton-1, a mixture of LAB; and T5: 2 g ton1, a mixture LAB+enzyme, LEN. Barley supplementation to frosted maize increased dry matter (DM) (p &lt; 0.01). The pH value was higher in LAB group (p &lt; 0.01). Treatments supplemented LAB and LEN significantly reduced crude protein contents (p &lt; 0.001). Lactic acid contents decreased in all treatments as compared to control group and ranged between 2.46 and 5.40% DM (p &lt; 0.05). The metabolisable energy content was significantly higher in B40 than in B20, LAB and LEN groups. Mean Fleig points showed with an “excellent” quality silage. In conclusion, supplementation of barley, LEN and inoculation of LAB to frosted maize had a good quality silage. The LEN supplementation may be used for efficient ensiling of frosted maize due to their ability to keep its quality in low DM crop maize silage.
DOI: 10.6084/m9.figshare.6991670.v1
title: Stability and adaptability for wood volume in the selection of Eucalyptus saligna in three environments

description: Abstract: The objective of this work was to evaluate the genotype x environment (GxE) interaction in open-pollinated Eucalyptus saligna progenies for simultaneous selection for greater stability and adaptability for wood volume, as well as to compare the selection strategies through combined and individual analyses in three environments. Three experiments were conducted in a randomized complete block design, with four replicates and six plants per plot, with 102 to 122 progenies. Three years after planting, survival rate and wood volume were evaluated. The genetic parameters were estimated by the maximum restricted likelihood and best linear unbiased prediction (REML/Blup) methods, and the stability and adaptability analysis was conducted using the harmonic mean of the relative performance of genetic values (HMRPGV). The highest survival rate (82%) was observed in Sabinópolis and the highest volume (120 m³ ha-1) in Lençóis Paulista. The mean heritability of progenies for the two analyzed variables was considered high, and the genetic correlations between survival rate and volume were weak for all sites. The G×E interaction was significant and of the complex type, with genotype correlation between sites of 47%, indicating that the ranking of the best progenies differed in each studied environment. The analysis of stability and adaptability indicated the possibility of selecting progenies with good performance in the three environments; however, to improve the quality of the evaluated characteristics, it is necessary to select specific progenies for each environment.
DOI: 10.6084/m9.figshare.6991664
title: The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells

description: It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be necessary to stimulate the increase in [Ca2+]i induced by gentamicin, since this stimulus disappeared by using 1.8 mM EGTA (a Ca2+ chelator). Morphological changes were observed with scanning electron microscopy in epithelial cells exposed to the antibiotic. Furthermore, with the MTT method, a decrease in metabolic activity induced by gentamicin was observed, which indicates a cytotoxic effect. In conclusion, gentamicin was able to alter [Ca2+]i, change the morphology of MDCK-C11 cells, and promote cytotoxicity.
DOI: 10.6084/m9.figshare.6991667
title: In-vitro assessment for ensilabillity of Tithonia diversifolia alone or with Pennisetum purpureum using epiphytic lactic acid bacteria strains as inocula

description: ABSTRACT. It is expected that the availability of forage for animals in the tropics will fluctuate in the future due to climate change. Ensiling of tropical forages constitutes a strategy to cope with food scarcity during long dry seasons. Tithonia diversifolia (TD), is a plant belonging to family Asteraceae, with a wide adaptation in tropical areas. A Rostock Fermentation Test (RFT) was performed to compare the ensilabillity potential among four epiphytic lactic acid bacteria (LAB) strains isolated from TD. Further, two experiments were designed 1) to evaluate the acidification potential of the best strain from the preliminary test (T735), and 2) to determine the ensilability of different levels of inclusion in the TD / PP mixture. For the first experiment, T735 and a blend of lactic acid-fermenting bacteria (BLB) decreased the pH rapidly. In experiment 2, different PP/TD ratios (0/100; 33/67; 67/33; 100/0) were ensiling, all inoculated with T735, (BLB) and their mixture. Increasing of grass PP in the mixtures brought about a fast drop in pH, facilitating TD ensilabillity. The use of T735 improved acidification and fermentation parameters of TD/PP silage.
DOI: 10.6084/m9.figshare.6991667.v1
title: In-vitro assessment for ensilabillity of Tithonia diversifolia alone or with Pennisetum purpureum using epiphytic lactic acid bacteria strains as inocula

description: ABSTRACT. It is expected that the availability of forage for animals in the tropics will fluctuate in the future due to climate change. Ensiling of tropical forages constitutes a strategy to cope with food scarcity during long dry seasons. Tithonia diversifolia (TD), is a plant belonging to family Asteraceae, with a wide adaptation in tropical areas. A Rostock Fermentation Test (RFT) was performed to compare the ensilabillity potential among four epiphytic lactic acid bacteria (LAB) strains isolated from TD. Further, two experiments were designed 1) to evaluate the acidification potential of the best strain from the preliminary test (T735), and 2) to determine the ensilability of different levels of inclusion in the TD / PP mixture. For the first experiment, T735 and a blend of lactic acid-fermenting bacteria (BLB) decreased the pH rapidly. In experiment 2, different PP/TD ratios (0/100; 33/67; 67/33; 100/0) were ensiling, all inoculated with T735, (BLB) and their mixture. Increasing of grass PP in the mixtures brought about a fast drop in pH, facilitating TD ensilabillity. The use of T735 improved acidification and fermentation parameters of TD/PP silage.
DOI: 10.6084/m9.figshare.6991664.v1
title: The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells

description: It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be necessary to stimulate the increase in [Ca2+]i induced by gentamicin, since this stimulus disappeared by using 1.8 mM EGTA (a Ca2+ chelator). Morphological changes were observed with scanning electron microscopy in epithelial cells exposed to the antibiotic. Furthermore, with the MTT method, a decrease in metabolic activity induced by gentamicin was observed, which indicates a cytotoxic effect. In conclusion, gentamicin was able to alter [Ca2+]i, change the morphology of MDCK-C11 cells, and promote cytotoxicity.
DOI: 10.6084/m9.figshare.6991658
title: Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

description: ABSTRACT Plant growth-promoting bacteria (PGPB) comprise part of plant microbiome of biotechnological interest due to their potential to decrease the use of agrochemicals in agriculture. Among the commonly found PGPB species, the Pseudomonas genus is known for high competitiveness and efficiency in expressing growth-promotion traits. To increase the contribution of diazotrophic Pseudomonas sp. to the plant nitrogen nutrition, the strain AZM-01 was chemically mutagenized with methyl methanesulfonate (MMS), following the selection for resistance to ethylenediamine (EDA). From the 13 EDA-resistant mutant strains selected, four showed increased the ammonium excretion, with the highest value reaching up to 284% increase as compared to the wild strain, and six strains were found to produce significantly more auxins than the wild strain. Two independent inoculation trials with the wild and EDA-resistant Pseudomonas were performed on maize, with the objective to study the influence of bacteria on seed germination and its potential to promote maize growth under N-limiting condition. In general, Pseudomonas inoculation modified the root architecture of germinating seeds, and increased biomass of maize plants grown under N-limiting conditions. Shoot dry weight of maize was increased by inoculation with several EDA-resistant mutants as compared to the strain AZM-01, with emphasis on the EDA-5 strain which supports biomass accumulation at equivalent amount of plants grown under full N supply. Significant correlations between in vitro and in vivo parameters were found although low coefficient values predominate. The strategy of random mutagenesis was found suitable to develop PGPB strains with higher potential to supply maize plants with nitrogen.
DOI: 10.6084/m9.figshare.6991661
title: HIV/AIDS case definition criteria and association between sociodemographic and clinical aspects of the disease reported in the State of Minas Gerais from 2007 to 2016

description: Abstract INTRODUCTION: Over 30 years after the acquired immunodeficiency syndrome epidemic, several strategies have been implemented to verify the trend of the infection, the profile of the affected individuals, and the impact of prevention and control measures, with notification of asymptomatic carriers being the most recent measure. This study aimed to verify the geographic distribution of human immunodeficiency virus/acquired immunodeficiency syndrome and analyze the association between case definition criteria, sociodemographic data, and clinical aspects of the disease in the State of Minas Gerais between 2007 and 2016. METHODS: In this ecological and analytical study, 35,349 cases of human immunodeficiency virus/acquired immunodeficiency syndrome reported in the State of Minas Gerais between 2007 and 2016 were analyzed. The data were analyzed using multiple correspondence factor analysis, time series analysis, descriptive statistics, and spatial distribution of the cases by macro-region. RESULTS: The majority of the patients were brown-skinned individuals, alive, diagnosed with human immunodeficiency virus/acquired immunodeficiency syndrome on the basis of the criteria adapted from the Centers for Disease Control and Prevention, and living in municipalities with more than 50,000 (80.5%) inhabitants. Between 2007 and 2016, there was an increase in the number of criteria used for diagnosing human immunodeficiency virus. By contrast, a consequent decrease was observed in the number of criteria used for defining cases, which were adapted from the Centers for Disease Control and Prevention, Rio de Janeiro/Caracas, and for identifying AIDS-related deaths. Young people aged between 13 and 29 years, individuals whose education level is compatible with the observed age, and homosexual men were associated with the HIV+ criterion. CONCLUSIONS: Out study showed that after the mandatory notification of human immunodeficiency virus-positive cases in 2014, there was a decrease in other criteria for defining human immunodeficiency virus/acquired immunodeficiency syndrome cases and changes in the profile of people living with human immunodeficiency virus/acquired immunodeficiency syndrome.
DOI: 10.6084/m9.figshare.6991658.v1
title: Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

description: ABSTRACT Plant growth-promoting bacteria (PGPB) comprise part of plant microbiome of biotechnological interest due to their potential to decrease the use of agrochemicals in agriculture. Among the commonly found PGPB species, the Pseudomonas genus is known for high competitiveness and efficiency in expressing growth-promotion traits. To increase the contribution of diazotrophic Pseudomonas sp. to the plant nitrogen nutrition, the strain AZM-01 was chemically mutagenized with methyl methanesulfonate (MMS), following the selection for resistance to ethylenediamine (EDA). From the 13 EDA-resistant mutant strains selected, four showed increased the ammonium excretion, with the highest value reaching up to 284% increase as compared to the wild strain, and six strains were found to produce significantly more auxins than the wild strain. Two independent inoculation trials with the wild and EDA-resistant Pseudomonas were performed on maize, with the objective to study the influence of bacteria on seed germination and its potential to promote maize growth under N-limiting condition. In general, Pseudomonas inoculation modified the root architecture of germinating seeds, and increased biomass of maize plants grown under N-limiting conditions. Shoot dry weight of maize was increased by inoculation with several EDA-resistant mutants as compared to the strain AZM-01, with emphasis on the EDA-5 strain which supports biomass accumulation at equivalent amount of plants grown under full N supply. Significant correlations between in vitro and in vivo parameters were found although low coefficient values predominate. The strategy of random mutagenesis was found suitable to develop PGPB strains with higher potential to supply maize plants with nitrogen.