DOI: 10.5061/dryad.tdz08kq7r
title: Odorant receptor sequences of Hyles lineata and Hyles vespertilio

description: In insects, odorant receptors (ORs) are required for the detection of most
 olfactory cues. Gene duplications leading to OR clade expansions may
 indicate chemosensory adaptations to ecological niches. Here, we
 investigated a clade of four intact duplicated ORs in the hawkmoth Manduca
 sexta. We tested these paralogs with 80 odorants and found that they
 encode broadly tuned receptors with overlapping but distinct response
 spectra. Two paralogs, which arose after divergence from related lineages,
 show high sensitivity to floral esters released by a nectar-rich plant
 frequently visited by M. sexta. Functional imaging in mutant moths lacking
 one of the paralogs suggests that olfactory sensory neurons expressing
 this OR target a previously identified feeding-associated glomerulus in
 the primary olfactory center of the brain. However, only the response of
 this glomerulus to the single ligand unique to the mutant OR disappeared,
 suggesting co-expression of the paralogs. Our results suggest a link
 between OR expansion and enhanced detection of odors emitted by valuable
 nectar sources in M. sexta.
description: The OR tandem arrays of two Hyles species were annotated in
 previously published genome assemblies (Godfrey et al. 2023; Pippel et al.
 (2020) )using a semi-automated pipeline developed for the annotation of
 multi-gene chemosensory gene families (Brand and Ramírez 2017).
   Godfrey, R.K., Britton, S.E., Mishra, S.,
 Goldberg, J.K., and Kawahara, A.Y. (2023). A high-quality, long-read
 genome assembly of the whitelined sphinx moth (Lepidoptera: Sphingidae:
 <em>Hyles lineata</em>) shows highly conserved melanin
 synthesis pathway genes. G3-Genes Genomes Genetics
 <em>13</em>. 10.1093/g3journal/jkad090.
 Pippel, M., Jebb, D., Patzold, F., Winkler, S., Vogel, H., Myers,
 G., Hiller, M., and Hundsdoerfer, A.K. (2020). A highly contiguous genome
 assembly of the bat hawkmoth <em>Hyles vespertilio</em>
 (Lepidoptera: Sphingidae). Gigascience <em>9</em>, giaa001.
 10.1093/gigascience/giaa001. Brand, P., and Ramírez,
 S.R. (2017). The evolutionary dynamics of the odorant receptor gene family
 in corbiculate bees. Genome Biology and Evolution <em>9</em>,
 2023-2036. 10.1093/gbe/evx149.
description: # Odorant receptor sequences of Hyles lineata and Hyles vespertilio
 [https://doi.org/10.5061/dryad.tdz08kq7r](https://doi.org/10.5061/dryad.tdz08kq7r) ## Description of the data and file structure Odorant receptor (OR) tandem arrays of two Hyles species (H. lineata and H. vespertilio) homologous to the OR33 tandem array of Manduca sexta were annotated in previously published genome assemblies using a semi-automated pipeline developed for the annotation of multi-gene chemosensory gene families. Each Hyles tandem array consists of four ORs. DNA and amino acid sequences of the ORs are saved as fasta files, and annotations corresponding to the published genomes are provided as gff3 files. Genome references: Godfrey, R.K., Britton, S.E., Mishra, S., Goldberg, J.K., and Kawahara, A.Y. (2023). A high-quality, long-read genome assembly of the whitelined sphinx moth (Lepidoptera: Sphingidae: *Hyles lineata*) shows highly conserved melanin synthesis pathway genes. G3-Genes Genomes Genetics *13*. 10.1093/g3journal/jkad090. Pippel, M., Jebb, D., Patzold, F., Winkler, S., Vogel, H., Myers, G., Hiller, M., and Hundsdoerfer, A.K. (2020). A highly contiguous genome assembly of the bat hawkmoth *Hyles vespertilio* Annotation pipeline: Brand, P., and Ramírez, S.R. (2017). The evolutionary dynamics of the odorant receptor gene family in corbiculate bees. Genome Biology and Evolution *9*, 2023-2036. 10.1093/gbe/evx149. ### Files and variables #### File: Hlin\_ORs\_v1.0\_AA.fa **Description:** Amino acid sequences of four H. lineata ORs #### File: Hlin\_ORs\_v1.0\_DNA.fa **Description:** DNA sequences of four H. lineata ORs #### File: Hves\_ORs\_v1.0\_AA.fa **Description:** Amino acid sequences of four H. vespertilio ORs #### File: Hves\_ORs\_v1.0\_DNA.fa **Description:** DNA sequences of four H. vespertilio ORs #### File: Hves\_ORs\_v1.0.gff **Description:** Annotation file of four H. vespertilio ORs #### File: Hlin\_ORs\_v1.0.gff **Description:** Annotation file of four H. lineata ORs ## Code/software Data can be viewed in sequence viewers such as geneious