cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/vgshw-3h971
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-21_slicer10328.jpg: Bdellovibrio bacteriovorus .  Nucleoid .  Flagellar motor .  chemoreceptor ycw2013-09-28-21_slicer14226.jpg: Bdellovibrio bacteriovirus .  Flagellar motor (arrow to c-ring?)
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-21/rawdata/</p>20130928_YWC034_10021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-21/rawdata/20130928_YWC034_10021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10021_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-21/3dimage_31390/20130928_YWC034_10021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z3cfz-ak602
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-27-12_slicer4297.jpg: Nucleoid ycw2013-09-27-12_slicer4298.jpg: Pilli ycw2013-09-27-12_slicer14184.jpg: flagellar motor; flagellum
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-12/rawdata/</p>20130827_YWC034_10012.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-12/rawdata/20130827_YWC034_10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130827_YWC034_10012_part131_7.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-12/3dimage_31327/20130827_YWC034_10012_part131_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/5zygg-jmj44
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2013-08-20</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett, Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
  ycw2013-08-20-13_slicer10307.jpg: nucleoid, Bdellovibrio bacteriovorus ycw2013-08-20-13_slicer10308.jpg: chemoreceptor array, Bdellovibrio bacteriovorus ycw2013-08-20-13_slicer14177.jpg: flagellar motor; flagellum
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-13/rawdata/</p>20130820_Bdellovibrio+WM3433_10003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-13/rawdata/20130820_Bdellovibrio+WM3433_10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130820_Bdellovibrio+WM3433_10003_part131_7.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-13/3dimage_29868/20130820_Bdellovibrio+WM3433_10003_part131_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wvvs5-88n84
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-28-37_slicer10355.jpg: Bdellovibrio bacteriovorus .  flagellum ycw2013-09-28-37_slicer10356.jpg: Bdellovibrio bacteriovorus .  Vesicle budding off of flagellum
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-37/rawdata/</p>20130928_YWC034_10037.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-37/rawdata/20130928_YWC034_10037.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130928_YWC034_10037.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-37/3dimage_31421/20130928_YWC034_10037.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/amwfw-cnv85
title: H. pylori 26695 / Hs.746T

description: Grid box YWC057
description: <strong>Tilt Series Date:</strong> 2013-12-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori 26695 from ATCC, prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.</p>
  ycw2013-12-16-46_slicer9942.jpg: Helicobacter pylori .  unknown filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-46/rawdata/</p>20131216_YWC057_20006.mrc,  Tilt Series (Pixel Size 0.48 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-46/rawdata/20131216_YWC057_20006.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131216_YWC057_20006_part87_1.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-16-46/3dimage_36198/20131216_YWC057_20006_part87_1.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.6084/m9.figshare.30007357
title: data of Digital Financial Inclusion and Urban Economic Resilience

description: This dataset was collected, processed, and utilized in a study on the Impact of Digital Inclusive Finance on Economic Resilience in the Beijing-Tianjin-Hebei Urban Agglomeration. It includes an indicator system, raw data, and processed data.
DOI: 10.6084/m9.figshare.30007357.v1
title: data of Digital Financial Inclusion and Urban Economic Resilience

description: This dataset was collected, processed, and utilized in a study on the Impact of Digital Inclusive Finance on Economic Resilience in the Beijing-Tianjin-Hebei Urban Agglomeration. It includes an indicator system, raw data, and processed data.
DOI: 10.22002/36x1e-dcb37
title: Bdellovibrio2492/E.coli minicell

description: Bdellovibrio HI Strain 2492 in mixture with E. coli WM3433 minicellsGrid box YWC107
description: <strong>Tilt Series Date:</strong> 2014-07-18</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio HI 2492 grown from frozen stocks in liquid PY media at 37C for 7 days, subculturing half of culture into equal volume of fresh media every two days. E. coli minicells WM3433 grown O/N to high OD and spun down 7 mins at 6krpm.  Supernatant spun 10 mins at 14krpm to pellet minicells.  Minicells resuspended in 20ul media and combined in 1:1 v/v ratio with Bdellovibrio.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. Manual blotting, 3 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-10/rawdata/</p>20140718_Janelia_YWC107_10011.mrc,  Tilt Series (Pixel Size 0.33 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-10/rawdata/20140718_Janelia_YWC107_10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140718_Janelia_YWC107_10011_full.rec,  Reconstruction (Pixel Size 1.32 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-18-10/3dimage_45090/20140718_Janelia_YWC107_10011_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4szw2-vqc45
title: E.coli co-overexpression

description: E.coli co overexpression UU1607/pJC3/pPM25
description: <strong>Tilt Series Date:</strong> 2013-07-17</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -3.0 μm,  magnification: 41000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> strain sent from Heather Hodges (Kiessling lab), parent strain and plasmids published from the Parkinson lab</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew UU1607/pJC3/pPM25 overnight in 5mL TB + 3.8µl Cm + 5µl CarbDiluted back 1:50 into 200mL TB + 152µl Cm + 200µl Carb + 50µl 1M IPTG + 200µl Na-salicylateGrew ~2 hours to mid-log phaseSpun down 10' at 5,000 x gResuspended in 300µl lysis buffer + 0.1µl PMSF + 0.75 µl 1,10-phenanthrolineAdded 33.3µl (2mg/ml) fresh lysozyme solution (20mg/ml in 10mM Tris-HCl, pH 8.0)Incubated 45' at 37°CAdded 1.67µl 1M MgCl2, 0.2µl 1M CaCl2, 0.33µl DNase IIncubated 30' at 37°CSpun down 4000 g 5 min Resuspended in activity buffer (cold room) without glycerol</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-6/rawdata/</p>cooverexpressionatlas4micronb0006.mrc,  Tilt Series (Pixel Size 0.28 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-6/rawdata/cooverexpressionatlas4micronb0006.mrc"
        > <i class="download icon"></i> Download </a></p>

        cooverexpressionatlas4micronb0006_full.rec,  Reconstruction (Pixel Size 0.56 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-07-17-6/3dimage_28358/cooverexpressionatlas4micronb0006_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sxe7n-yr613
title: Bdellovibrio attack-phase

description: Raw data files of Bdellovibrio attack-phase
description: <strong>Tilt Series Date:</strong> 2013-08-20</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Liz Sockett, Cells prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid</p>
  ycw2013-08-20-12_slicer10303.jpg: flagellum, Bdellovibrio bacteriovorus ycw2013-08-20-12_slicer10304.jpg: chemoreceptor, Bdellovibrio bacteriovorus ycw2013-08-20-12_slicer10305.jpg: nucleoid, Bdellovibrio bacteriovorus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-12/rawdata/</p>20130820_Bdellovibrio+WM3433_10002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-12/rawdata/20130820_Bdellovibrio+WM3433_10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130820_Bdellovibrio+WM3433_10002_part131_7.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-08-20-12/3dimage_29866/20130820_Bdellovibrio+WM3433_10002_part131_7.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dpqnz-8yj15
title: H.pylori 26695 delta-cagH / AGS

description: grid box: YWC087Hp cell were scraped from agar plate, grown few more hours in BLBB, not happy
description: <strong>Tilt Series Date:</strong> 2014-02-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> H.pylori & AGS cells from Carrie Shaffer</p>
<strong>Purification / Growth Conditions / Treatment:</strong> H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-16-22/rawdata/</p>20140216_YWC087_10022.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-16-22/rawdata/20140216_YWC087_10022.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140216_YWC087_10022.rec,  Reconstruction (Pixel Size 1.92 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-16-22/3dimage_39032/20140216_YWC087_10022.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ezyxn-m6190
title: Bdellovibrio/E. coli minicell

description: Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
description: <strong>Tilt Series Date:</strong> 2013-09-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Bdellovibrio bacteriovorus</p>
<strong>Strain:</strong> HD100</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder</p>
  ycw2013-09-27-13_slicer4309.jpg: Nucleoid ycw2013-09-27-13_slicer4312.jpg: external vesicles ycw2013-09-27-13_slicer14185.jpg: flagellar motor; flagellum
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-27-13/rawdata/</p>20130827_YWC034_10013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
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        20130827_YWC034_10013_part131_7.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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