cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5792259.v1
title: Stigma receptivity and anther dehiscence in ornamental pepper

description: ABSTRACT The objective of this study was to evaluate stigma receptivity and anther dehiscence in three floral development stages in F3 population of ornamental pepper (Capsicum annuum). A total of 162 plants (81 of purple corolla and 81 of white corolla) were used in this study. To analyze the stigma receptivity and anther dehiscence, three floral stages were analyzed: bud, pre-anthesis, and open flower. For each stage, 33 flowers of each phenotypic class were collected. The receptivity of the stigma was detected by dripping hydrogen peroxide solution (3%). For the evaluation of the level of receptivity, scores of 0 to 3 were assigned, varying according to the bubbles percentage found on the stigmatic surface. Scores of 1 to 5 were assigned for the reference of the amount of pollen released by the stamens. The data were subjected to analysis of variance (p≤0.05) following a 2x3 factorial scheme: two classes of flower colors and three development stages, with 33 replicates (flowers) with later separation of means by Tukey’s test at the same probability level. For the analysis of divergence, the data were clustered according to Tocher’s method based on generalized Mahalanobis distance. Despite the not significant differences between the means for pollen dehiscence of both flower colors, the purple flowers presented higher stigma receptivity than the white ones. The ornamental pepper flowers displayed stigma receptivity since the bud phase, but the highest level of receptivity was observed after anthesis. Anther dehiscence prevailed after anthesis of the flowers regardless of color, and the presence of pollen grains was superior after anthesis. For the manual crossing, the buds at pre-anthesis phase are the most indicated irrespective of the color, as they show high stigma receptivity and greater ease at handling.
DOI: 10.6084/m9.figshare.5792256
title: The long and successful journey of electrochemically active amino acids. From fundamental adsorption studies to potential surface engineering tools.

description: ABSTRACT Proteins have been the subject of electrochemical studies. It is possible to apply electrochemical techniques to obtain information about their structure due to the presence of five electroactive amino acids that can be oriented to the outside of the peptidic chain. These amino acids are L-Tryptophan (L-Trp), L-Tyrosine (L-Tyr), L-Histidine (L-His), L-Methionine (L-Met) and L-Cysteine (L-Cys); their electrochemical behavior being subject of extensive research, but it is still controversial. No spectroscopic investigations have been reported on L-Trp, and due to the short life time of the intermediates, ex situ techniques cannot be employed, leading to a never-ending discussion about possible intermediates. In the L-Tyr and L-His cases, spectroelectrochemical studies were performed and different intermediates were observed, suggesting that some intermediates may be observed under specific conditions, as proposed for L-Cys. This amino acid is the most interesting among the electroactive ones because of the presence of a thiol moiety at its side chain, leading to a wide range of oxidation states. It can adsorb onto surfaces of different crystallographic orientation in stereoselective conformation, modifying the surface for different applications.as a surface engineering tool since it plays the role of as an anchor for the growing of nanocrystals inside proteic templates.
DOI: 10.6084/m9.figshare.5792256.v1
title: The long and successful journey of electrochemically active amino acids. From fundamental adsorption studies to potential surface engineering tools.

description: ABSTRACT Proteins have been the subject of electrochemical studies. It is possible to apply electrochemical techniques to obtain information about their structure due to the presence of five electroactive amino acids that can be oriented to the outside of the peptidic chain. These amino acids are L-Tryptophan (L-Trp), L-Tyrosine (L-Tyr), L-Histidine (L-His), L-Methionine (L-Met) and L-Cysteine (L-Cys); their electrochemical behavior being subject of extensive research, but it is still controversial. No spectroscopic investigations have been reported on L-Trp, and due to the short life time of the intermediates, ex situ techniques cannot be employed, leading to a never-ending discussion about possible intermediates. In the L-Tyr and L-His cases, spectroelectrochemical studies were performed and different intermediates were observed, suggesting that some intermediates may be observed under specific conditions, as proposed for L-Cys. This amino acid is the most interesting among the electroactive ones because of the presence of a thiol moiety at its side chain, leading to a wide range of oxidation states. It can adsorb onto surfaces of different crystallographic orientation in stereoselective conformation, modifying the surface for different applications.as a surface engineering tool since it plays the role of as an anchor for the growing of nanocrystals inside proteic templates.
DOI: 10.6084/m9.figshare.5792247
title: Comb, cloaca and feet scores and testis morphometry in male broiler breeders at two different ages

description: ABSTRACT In broiler breeder roosters, the weight of the testes is positively associated with daily sperm production and fertility. In birds, the testes are located in the coelomic cavity, not being accessible to reproductive evaluation as mammalian testes. The reproductive evaluation of roosters is based on phenotypic traits. Any changes on testes will be reflected on fertility levels. The aim of this paper was to evaluate the potential of association of phenotypic traits as body weight (BW), comb scores (COS), cloaca scores (CLS) and feet scores (FS) with testicular morphometric parameters in adult Cobb® Mx roosters in two ages (25 and 45 weeks of age). The low BW roosters had lower testes weight (TW), smaller seminiferous tubule diameter (STD) and lower comb and cloaca scores than the medium and high BW roosters (P≤0.05). Heavy roosters had the highest COS and bigger seminiferous epithelium height (SEH). There was no statistical difference between the BW categories in FS and volumetric proportion of seminiferous tubule (ST) and interstitial tissue (IT). Considering the age effect, roosters with 45 weeks of age had lower TW and ST than 25-week roosters. Positive, moderate and significant correlation was found between testes weight and phenotypic characteristics as BW, COS and CLS in 45week roosters. A positive, moderate and significant correlation was found between body weight and histologic testicular characteristics as STD and SHE in both evaluated ages. In conclusion, there is a regression in the testes weight with age and it is more intense in Cobb® Mx roosters with lower BW. Furthermore, it is concluded that the BW positively influences the testes weight and histological quality of the testes. COS and CLS can be used with moderate potential prediction to identify roosters with low testes weight at 45 weeks of age.
DOI: 10.6084/m9.figshare.5792247.v1
title: Comb, cloaca and feet scores and testis morphometry in male broiler breeders at two different ages

description: ABSTRACT In broiler breeder roosters, the weight of the testes is positively associated with daily sperm production and fertility. In birds, the testes are located in the coelomic cavity, not being accessible to reproductive evaluation as mammalian testes. The reproductive evaluation of roosters is based on phenotypic traits. Any changes on testes will be reflected on fertility levels. The aim of this paper was to evaluate the potential of association of phenotypic traits as body weight (BW), comb scores (COS), cloaca scores (CLS) and feet scores (FS) with testicular morphometric parameters in adult Cobb® Mx roosters in two ages (25 and 45 weeks of age). The low BW roosters had lower testes weight (TW), smaller seminiferous tubule diameter (STD) and lower comb and cloaca scores than the medium and high BW roosters (P≤0.05). Heavy roosters had the highest COS and bigger seminiferous epithelium height (SEH). There was no statistical difference between the BW categories in FS and volumetric proportion of seminiferous tubule (ST) and interstitial tissue (IT). Considering the age effect, roosters with 45 weeks of age had lower TW and ST than 25-week roosters. Positive, moderate and significant correlation was found between testes weight and phenotypic characteristics as BW, COS and CLS in 45week roosters. A positive, moderate and significant correlation was found between body weight and histologic testicular characteristics as STD and SHE in both evaluated ages. In conclusion, there is a regression in the testes weight with age and it is more intense in Cobb® Mx roosters with lower BW. Furthermore, it is concluded that the BW positively influences the testes weight and histological quality of the testes. COS and CLS can be used with moderate potential prediction to identify roosters with low testes weight at 45 weeks of age.
DOI: 10.6084/m9.figshare.5792241
title: Identification of collagens types I, III, IV and α-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis

description: ABSTRACT Endometriosis is a degenerative change of the uterine glands and surrounding stroma, characterized by periglandular arrangement of myofibroblasts and deposition of extracellular matrix (ECM). The aim of this study was to evaluate the expression of collagen type I, III and IV and α-smooth muscle actin (α-SMA) in equine endometriosis, and investigate the role of myofibroblasts in the progression of these processes. A parallel was made with histochemical techniques of Masson's trichrome, Picrosirius Red under polarized light and Periodic Acid-Schiff (PAS). Twenty four uterine biopsies received by the Veterinary Pathology Service and Animal Reproduction of FMVZ, UNESP, Botucatu, SP, were diagnosed with endometriosis. Histological analysis revealed that the orange dense collagen fibers correspond to type I collagen, being prevalent in inactive and inactive destructive endometriosis. The green loose collagen fibers correspond to type III collagen, and are predominant in active and active destructive endometriosis. In the same processes, a greater amount of periglandular myofibroblasts were observed in comparison to inactive and inactive destructive endometriosis. The presence of these cells in active processes are strongly related to an increased deposition of collagen type III in fibrotic nests. Regarding the basement membrane, the active destructive and active endometriosis shows thickening, apparently not related to an increase in expression of type IV collagen. The active destructive and inactive destructive endometriosis exhibited disruption areas in type IV collagen fibers. Thus, it is noted that the myofibroblasts are related to increased deposition of type III collagen in active fibrotic nests.
DOI: 10.6084/m9.figshare.5792241.v1
title: Identification of collagens types I, III, IV and α-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis

description: ABSTRACT Endometriosis is a degenerative change of the uterine glands and surrounding stroma, characterized by periglandular arrangement of myofibroblasts and deposition of extracellular matrix (ECM). The aim of this study was to evaluate the expression of collagen type I, III and IV and α-smooth muscle actin (α-SMA) in equine endometriosis, and investigate the role of myofibroblasts in the progression of these processes. A parallel was made with histochemical techniques of Masson's trichrome, Picrosirius Red under polarized light and Periodic Acid-Schiff (PAS). Twenty four uterine biopsies received by the Veterinary Pathology Service and Animal Reproduction of FMVZ, UNESP, Botucatu, SP, were diagnosed with endometriosis. Histological analysis revealed that the orange dense collagen fibers correspond to type I collagen, being prevalent in inactive and inactive destructive endometriosis. The green loose collagen fibers correspond to type III collagen, and are predominant in active and active destructive endometriosis. In the same processes, a greater amount of periglandular myofibroblasts were observed in comparison to inactive and inactive destructive endometriosis. The presence of these cells in active processes are strongly related to an increased deposition of collagen type III in fibrotic nests. Regarding the basement membrane, the active destructive and active endometriosis shows thickening, apparently not related to an increase in expression of type IV collagen. The active destructive and inactive destructive endometriosis exhibited disruption areas in type IV collagen fibers. Thus, it is noted that the myofibroblasts are related to increased deposition of type III collagen in active fibrotic nests.
DOI: 10.6084/m9.figshare.5792238
title: A case of severe glutathione synthetase deficiency with novel GSS mutations

description: Glutathione synthetase deficiency (GSSD) is a rare inborn error of glutathione metabolism with autosomal recessive inheritance. The severe form of the disease is characterized by acute metabolic acidosis, usually present in the neonatal period with hemolytic anemia and progressive encephalopathy. A case of a male newborn infant who had severe metabolic acidosis with high anion gap, hemolytic anemia, and hyperbilirubinemia is reported. A high level of 5-oxoproline was detected in his urine and a diagnosis of generalized GSSD was made. DNA sequence analysis revealed the infant to be compound heterozygous with two mutations, c.738dupG in exon 8 of GSS gene resulting in p.S247fs and a repetitive sequence in exon 3 of GSS gene. Treatment after diagnosis of GSSD included supplementation with antioxidants and oral sodium hydrogen bicarbonate. However, he maintained a variable degree of metabolic acidosis and succumbed shortly after his parents requested discontinuation of therapy because of dismal prognosis and medical futility when he was 18 days old.
DOI: 10.6084/m9.figshare.5792238.v1
title: A case of severe glutathione synthetase deficiency with novel GSS mutations

description: Glutathione synthetase deficiency (GSSD) is a rare inborn error of glutathione metabolism with autosomal recessive inheritance. The severe form of the disease is characterized by acute metabolic acidosis, usually present in the neonatal period with hemolytic anemia and progressive encephalopathy. A case of a male newborn infant who had severe metabolic acidosis with high anion gap, hemolytic anemia, and hyperbilirubinemia is reported. A high level of 5-oxoproline was detected in his urine and a diagnosis of generalized GSSD was made. DNA sequence analysis revealed the infant to be compound heterozygous with two mutations, c.738dupG in exon 8 of GSS gene resulting in p.S247fs and a repetitive sequence in exon 3 of GSS gene. Treatment after diagnosis of GSSD included supplementation with antioxidants and oral sodium hydrogen bicarbonate. However, he maintained a variable degree of metabolic acidosis and succumbed shortly after his parents requested discontinuation of therapy because of dismal prognosis and medical futility when he was 18 days old.
DOI: 10.6084/m9.figshare.5792235
title: Physiological quality and seed respiration of primed Jatropha curcas seeds

description: ABSTRACT Seed deterioration is a natural and irreversible process. Nevertheless, seed priming with water and antioxidants can minimize oxidative damage in oilseeds, resulting in attenuation of seed deterioration. The objective of this assay was to evaluate seed priming on respiratory activity of Jatropha curcas submitted to accelerated aging. Seeds from two provenances (Janauba and Pedro J. Caballero) were submitted to three priming treatments (control, immersion in deionized water, and with 750 µmol L-1 of ascorbic acid) and treated for accelerated aging at 41 °C for 72 h. The results showed that the priming of J. curcas seeds promoted tolerance to accelerated aging. Primed seeds, with ascorbic acid from Janauba and deionized water from Pedro J. Caballero, resulted in a higher percentage of normal seedlings, and increased germination speed index and seed respiration. The decline of physiological quality of J. curcas seeds after accelerated aging is directly associated with a reduction in respiratory activity that is related to seed moisture content.
DOI: 10.6084/m9.figshare.5792235.v1
title: Physiological quality and seed respiration of primed Jatropha curcas seeds

description: ABSTRACT Seed deterioration is a natural and irreversible process. Nevertheless, seed priming with water and antioxidants can minimize oxidative damage in oilseeds, resulting in attenuation of seed deterioration. The objective of this assay was to evaluate seed priming on respiratory activity of Jatropha curcas submitted to accelerated aging. Seeds from two provenances (Janauba and Pedro J. Caballero) were submitted to three priming treatments (control, immersion in deionized water, and with 750 µmol L-1 of ascorbic acid) and treated for accelerated aging at 41 °C for 72 h. The results showed that the priming of J. curcas seeds promoted tolerance to accelerated aging. Primed seeds, with ascorbic acid from Janauba and deionized water from Pedro J. Caballero, resulted in a higher percentage of normal seedlings, and increased germination speed index and seed respiration. The decline of physiological quality of J. curcas seeds after accelerated aging is directly associated with a reduction in respiratory activity that is related to seed moisture content.
DOI: 10.6084/m9.figshare.5792232
title: Women empowerment through social innovation in indigenous social enterprises

description: ABSTRACT Purpose: To explore innovative enabler mechanisms for women's empowerment in a social enterprise and how they promote local development in a Zapotec indigenous community, the third largest ethnic group in Mexico. Originality/gap/relevance/implications: This paper contributes to the extension of social entrepreneurship literature from a gender perspective, exploring the mechanisms that allow women to succeed in highly marginalized indigenous communities. Key methodological aspects: This paper follows a case study methodology, inductive approach and qualitative methods mainly through 70 in-depth interviews. Summary of key results: Although the male-dominated culture slows down the democratic and political empowerment of women in the community, mechanisms such as job stability, low-interest microcredits and gender-equality policies in the organization have triggered economic empowerment. Key considerations/conclusions: The creation of empowering mechanisms within the social enterprise has allowed the Zapotec community to prosper and increase its general wellbeing. Women have been particularly benefited since the organization has given them the opportunity to work, empowering them to create micro-enterprises and changing the prevailing culture towards a more equalitarian society. Increasing control of their source of income has improved women's willingness to participate in political and managerial decision-making, inspiring more women in the community to work at the organization.
DOI: 10.6084/m9.figshare.5792232.v1
title: Women empowerment through social innovation in indigenous social enterprises

description: ABSTRACT Purpose: To explore innovative enabler mechanisms for women's empowerment in a social enterprise and how they promote local development in a Zapotec indigenous community, the third largest ethnic group in Mexico. Originality/gap/relevance/implications: This paper contributes to the extension of social entrepreneurship literature from a gender perspective, exploring the mechanisms that allow women to succeed in highly marginalized indigenous communities. Key methodological aspects: This paper follows a case study methodology, inductive approach and qualitative methods mainly through 70 in-depth interviews. Summary of key results: Although the male-dominated culture slows down the democratic and political empowerment of women in the community, mechanisms such as job stability, low-interest microcredits and gender-equality policies in the organization have triggered economic empowerment. Key considerations/conclusions: The creation of empowering mechanisms within the social enterprise has allowed the Zapotec community to prosper and increase its general wellbeing. Women have been particularly benefited since the organization has given them the opportunity to work, empowering them to create micro-enterprises and changing the prevailing culture towards a more equalitarian society. Increasing control of their source of income has improved women's willingness to participate in political and managerial decision-making, inspiring more women in the community to work at the organization.
DOI: 10.6084/m9.figshare.5792226
title: Effect of high Cu2+ stress on fermentation performance and copper biosorption of Saccharomyces cerevisiae during wine fermentation

description: Abstract The effect of high Cu2+ stress on fermentation performance and copper biosorption of Saccharomyces cerevisiae BH8 during wine fermentation was investigated in this paper. Under high Cu2+ stress, the cell growth and survival rate of yeast BH8 were inhibited. With different copper concentration, the effect on cell growth of yeast BH8 was different. What’s more, high Cu2+ stress could inhibit the fermentation property, alcohol production and reducing sugar utilization of yeast BH8 in Chardonnay grape must, but not significant. And the trend of the copper ion concentration falling down was consistent with the trend of yeast growth rising up, meanwhile the copper content was still much less than the initial concentration after fermentation. In addition, yeast BH8 showed a good fermenting property and copper removal ability under high Cu2+ stress. These results could provide certain reference and some new data for the wine industry to face the copper pollution risk.
DOI: 10.6084/m9.figshare.5792226.v1
title: Effect of high Cu2+ stress on fermentation performance and copper biosorption of Saccharomyces cerevisiae during wine fermentation

description: Abstract The effect of high Cu2+ stress on fermentation performance and copper biosorption of Saccharomyces cerevisiae BH8 during wine fermentation was investigated in this paper. Under high Cu2+ stress, the cell growth and survival rate of yeast BH8 were inhibited. With different copper concentration, the effect on cell growth of yeast BH8 was different. What’s more, high Cu2+ stress could inhibit the fermentation property, alcohol production and reducing sugar utilization of yeast BH8 in Chardonnay grape must, but not significant. And the trend of the copper ion concentration falling down was consistent with the trend of yeast growth rising up, meanwhile the copper content was still much less than the initial concentration after fermentation. In addition, yeast BH8 showed a good fermenting property and copper removal ability under high Cu2+ stress. These results could provide certain reference and some new data for the wine industry to face the copper pollution risk.