cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30364209
title: Bovine Lymphocyte Intestinal Retention Defect (BLIRD): a novel recessive immunogenetic disorder in Holstein cattle

description: Dozens of missed recessive loci affecting homozygous carriers’ life expectancy were recently reported. This article details the clinical, biological and pathological manifestations of a new bovine genetic disorder caused by the ITGB7 p.G375S point mutation in the French Holstein cattle breed (BLIRD: OMIA:002872–9913). Our thorough study involved database analysis of genotyped cattle and a series of case-control investigations of forty individuals homozygous for the causative variant. These variant homozygotes had a significantly shorter lifespan (fewer than 64% surviving past three years vs. 87% in control), along with reduced body weight, daily weight gain, and dairy performance. The mutation did not affect most biochemical parameters, but a marked lymphocytic leucocytosis, moderate eosinophilia and differences in faecal microbiota were observed. Although non-pathognomonic symptoms may be confused with those of common environmental diseases, the blood profile effectively identified suspected carriers who developed ill-thrift and poor growth as heifers. Our research demonstrates that the bovine ITGB7 p.G375S substitution leads to reduced longevity, poor condition and production in most homozygous carriers. Furthermore, this spontaneous model may help to refine the functions of the integrin β7 (ITGB7) in immune homeostasis and defence.
DOI: 10.6084/m9.figshare.30364209.v1
title: Bovine Lymphocyte Intestinal Retention Defect (BLIRD): a novel recessive immunogenetic disorder in Holstein cattle

description: Dozens of missed recessive loci affecting homozygous carriers’ life expectancy were recently reported. This article details the clinical, biological and pathological manifestations of a new bovine genetic disorder caused by the ITGB7 p.G375S point mutation in the French Holstein cattle breed (BLIRD: OMIA:002872–9913). Our thorough study involved database analysis of genotyped cattle and a series of case-control investigations of forty individuals homozygous for the causative variant. These variant homozygotes had a significantly shorter lifespan (fewer than 64% surviving past three years vs. 87% in control), along with reduced body weight, daily weight gain, and dairy performance. The mutation did not affect most biochemical parameters, but a marked lymphocytic leucocytosis, moderate eosinophilia and differences in faecal microbiota were observed. Although non-pathognomonic symptoms may be confused with those of common environmental diseases, the blood profile effectively identified suspected carriers who developed ill-thrift and poor growth as heifers. Our research demonstrates that the bovine ITGB7 p.G375S substitution leads to reduced longevity, poor condition and production in most homozygous carriers. Furthermore, this spontaneous model may help to refine the functions of the integrin β7 (ITGB7) in immune homeostasis and defence.
DOI: 10.6084/m9.figshare.28623010
title: Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle

description: Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.
DOI: 10.6084/m9.figshare.28623010.v1
title: Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle

description: Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.
DOI: 10.6084/m9.figshare.28740808
title: High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings

description: The diagnosis and management of lumbosacral pain in dogs is challenging, requiring thorough examination, with MRI playing a crucial diagnostic role. This retrospective study investigates the presence of high-intensity zones (HIZ) in the dorsal annulus fibrosus (AF) of the lumbosacral region on MRI and describes the corresponding histopathological features in dogs with intervertebral disc (IVD) degeneration. T2-weighted (T2W) and T1-weighted (T1W) sagittal MRI scans were evaluated using a classification system developed in human medicine to analyze HIZ characteristics. Among 836 dogs with IVD degeneration, 57 (6.8%) exhibited T2W HIZ, with a median age of 7 years and median weight of 33.7 kg. All cases with HIZ consistently exhibited radiological degenerative lumbosacral stenosis. The most common T2W HIZ shape was round (43%), while 14% of lesions also appeared hyperintense on T1W. Histopathological analysis of 11 dorsal AF samples collected during standard-of-care decompressive surgery revealed two patterns: reactive cystic structures (3/11) and granulation tissue (8/11), with differential MRI presentation. This is the first study to document HIZ in the lumbosacral level of dogs with IVD degeneration. With this recognition, prospective analyses and their correlation with clinical presentations will be essential in determining the role and prognostic significance of HIZ.
DOI: 10.6084/m9.figshare.28740808.v1
title: High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings

description: The diagnosis and management of lumbosacral pain in dogs is challenging, requiring thorough examination, with MRI playing a crucial diagnostic role. This retrospective study investigates the presence of high-intensity zones (HIZ) in the dorsal annulus fibrosus (AF) of the lumbosacral region on MRI and describes the corresponding histopathological features in dogs with intervertebral disc (IVD) degeneration. T2-weighted (T2W) and T1-weighted (T1W) sagittal MRI scans were evaluated using a classification system developed in human medicine to analyze HIZ characteristics. Among 836 dogs with IVD degeneration, 57 (6.8%) exhibited T2W HIZ, with a median age of 7 years and median weight of 33.7 kg. All cases with HIZ consistently exhibited radiological degenerative lumbosacral stenosis. The most common T2W HIZ shape was round (43%), while 14% of lesions also appeared hyperintense on T1W. Histopathological analysis of 11 dorsal AF samples collected during standard-of-care decompressive surgery revealed two patterns: reactive cystic structures (3/11) and granulation tissue (8/11), with differential MRI presentation. This is the first study to document HIZ in the lumbosacral level of dogs with IVD degeneration. With this recognition, prospective analyses and their correlation with clinical presentations will be essential in determining the role and prognostic significance of HIZ.
DOI: 10.6084/m9.figshare.30344596
title: Glucose-6-phosphatase catalytic subunit 3 enhances host resistance to porcine epidemic diarrhea virus through regulating glycolysis/gluconeogenesis

description: Porcine epidemic diarrhea virus (PEDV) causes a highly contagious disease in pigs, and the intricacies of its host–pathogen interactions require further elucidation. Chinese Min piglets, known for their superior resistance to stress and disease, were compared with Yorkshire piglets to investigate breed-specific resistance mechanisms. We established PEDV infection models in both breeds and analyzed differences by assessing cytokine levels, viral loads, and histological changes in jejunal tissues. Transcriptomic analysis of jejunal tissues identified 5422 differentially expressed (DE) protein-coding genes (PCGs) and 1999 DE long non-coding RNAs (lncRNAs) between the two pig breeds. Functional annotation revealed that Yorkshire piglets exhibited upregulation of inflammatory and apoptotic pathways, whereas Chinese Min piglets displayed strong inflammatory responses and enhanced mucosal immunity. Notably, glucose-6-phosphatase catalytic subunit 3 (<i>G6PC3</i>) expression was significantly higher in Chinese Min piglets than in Yorkshire piglets. Knockdown of <i>G6PC3</i> in the intestinal porcine epithelial cell line J2 (IPEC-J2) resulted in increased PEDV replication and decreased expression of immune-related genes involved in the glycolysis/gluconeogenesis metabolism pathway. These findings highlight the distinct immune responses of Chinese Min and Yorkshire piglets to PEDV infection, and identify key PCGs and lncRNAs associated with PEDV immunity.
DOI: 10.6084/m9.figshare.30344596.v1
title: Glucose-6-phosphatase catalytic subunit 3 enhances host resistance to porcine epidemic diarrhea virus through regulating glycolysis/gluconeogenesis

description: Porcine epidemic diarrhea virus (PEDV) causes a highly contagious disease in pigs, and the intricacies of its host–pathogen interactions require further elucidation. Chinese Min piglets, known for their superior resistance to stress and disease, were compared with Yorkshire piglets to investigate breed-specific resistance mechanisms. We established PEDV infection models in both breeds and analyzed differences by assessing cytokine levels, viral loads, and histological changes in jejunal tissues. Transcriptomic analysis of jejunal tissues identified 5422 differentially expressed (DE) protein-coding genes (PCGs) and 1999 DE long non-coding RNAs (lncRNAs) between the two pig breeds. Functional annotation revealed that Yorkshire piglets exhibited upregulation of inflammatory and apoptotic pathways, whereas Chinese Min piglets displayed strong inflammatory responses and enhanced mucosal immunity. Notably, glucose-6-phosphatase catalytic subunit 3 (<i>G6PC3</i>) expression was significantly higher in Chinese Min piglets than in Yorkshire piglets. Knockdown of <i>G6PC3</i> in the intestinal porcine epithelial cell line J2 (IPEC-J2) resulted in increased PEDV replication and decreased expression of immune-related genes involved in the glycolysis/gluconeogenesis metabolism pathway. These findings highlight the distinct immune responses of Chinese Min and Yorkshire piglets to PEDV infection, and identify key PCGs and lncRNAs associated with PEDV immunity.
DOI: 10.6084/m9.figshare.28587658
title: Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal<sup>®</sup> tissue sealer in pond sliders (<i>Trachemys scripta</i>): impacts on procedure time, surgical access, and recovery time according to follicular stage

description: Pond sliders (<i>Trachemys scripta</i>) is a common pet and invasive species in many countries. Its ability to thrive and reproduce in Mediterranean climates necessitates population control to prevent overpopulation. Ovariectomy is considered as a preventive strategy to limit reproduction and protect local biodiversity. This study compares two spaying techniques in <i>Trachemys scripta</i>: electrosurgery using the EnSeal<sup>®</sup> tissue sealer (group E) and a conventional approach with manual ligation of blood vessels (group C). Statistical tests evaluated procedural differences, including ANOVA for duration (factoring in follicular stage, surgery type, and their interaction) and Chi-squared tests for the likelihood of single surgical access <i>via</i> the prefemoral fossa and post-surgical feeding. Pearson’s Chi-squared test with Yates’ correction was used to assess complications by surgery type and follicular stage. ANOVA assessed the effect of follicular stage on surgery time, with follicles classified as Class I (<i>n</i> = 17), Class II (<i>n</i> = 12), and Class III (<i>n</i> = 7). Both follicular stage and surgery type significantly impacted surgery time, while their interaction was not significant, indicating independent effects. The likelihood of single-access procedures was significantly linked to surgery type, with EnSeal<sup>®</sup> procedures being faster and associated with earlier post-surgical feeding. Complications were not significantly linked to surgery type or follicular stage.
DOI: 10.6084/m9.figshare.28587658.v1
title: Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal<sup>®</sup> tissue sealer in pond sliders (<i>Trachemys scripta</i>): impacts on procedure time, surgical access, and recovery time according to follicular stage

description: Pond sliders (<i>Trachemys scripta</i>) is a common pet and invasive species in many countries. Its ability to thrive and reproduce in Mediterranean climates necessitates population control to prevent overpopulation. Ovariectomy is considered as a preventive strategy to limit reproduction and protect local biodiversity. This study compares two spaying techniques in <i>Trachemys scripta</i>: electrosurgery using the EnSeal<sup>®</sup> tissue sealer (group E) and a conventional approach with manual ligation of blood vessels (group C). Statistical tests evaluated procedural differences, including ANOVA for duration (factoring in follicular stage, surgery type, and their interaction) and Chi-squared tests for the likelihood of single surgical access <i>via</i> the prefemoral fossa and post-surgical feeding. Pearson’s Chi-squared test with Yates’ correction was used to assess complications by surgery type and follicular stage. ANOVA assessed the effect of follicular stage on surgery time, with follicles classified as Class I (<i>n</i> = 17), Class II (<i>n</i> = 12), and Class III (<i>n</i> = 7). Both follicular stage and surgery type significantly impacted surgery time, while their interaction was not significant, indicating independent effects. The likelihood of single-access procedures was significantly linked to surgery type, with EnSeal<sup>®</sup> procedures being faster and associated with earlier post-surgical feeding. Complications were not significantly linked to surgery type or follicular stage.
DOI: 10.5281/zenodo.17923300
title: Physical Activity Levels on Functional Outcomes and Quality of Life in Musculoskeletal Rehabilitation.

description: Physical Activity Levels on Functional Outcomes and Quality of Life in Musculoskeletal Rehabilitation.
DOI: 10.5281/zenodo.14162314
title: Physical Activity Levels on Functional Outcomes and Quality of Life in Musculoskeletal Rehabilitation.

description: Physical Activity Levels on Functional Outcomes and Quality of Life in Musculoskeletal Rehabilitation.
DOI: 10.5281/zenodo.17923304
title: THE IMPORTANCE OF LIGHTING IN PHARMACIES. THE PHYSIOLOGICAL AND HYGIENIC IMPORTANCE OF ROOM INSULATION AND NATURAL LIGHTING
DOI: 10.5281/zenodo.17923303
title: THE IMPORTANCE OF LIGHTING IN PHARMACIES. THE PHYSIOLOGICAL AND HYGIENIC IMPORTANCE OF ROOM INSULATION AND NATURAL LIGHTING
DOI: 10.5281/zenodo.17923312
title: MODERN APPROACHES TO IMPROVING THE STATE SYSTEM OF MATERNAL AND CHILD PROTECTION FROM A BIOETHICAL PERSPECTIVE
DOI: 10.5281/zenodo.17923311
title: MODERN APPROACHES TO IMPROVING THE STATE SYSTEM OF MATERNAL AND CHILD PROTECTION FROM A BIOETHICAL PERSPECTIVE
DOI: 10.15468/dl.6aaf4f
title: Occurrence Download

description: A dataset containing 72263771 species occurrences available in GBIF matching the query:
{
 "and" : [
 "FamilyKey is one of (Acanthopteroctetidae, Acrolepiidae, Adelidae, Alucitidae, Apatelodidae, Archaeolepidae, Arctiidae, Argyresthiidae, Argyrestidae, Autostichidae, Batrachedridae, Bedelliidae, Blastobasidae, Bombycidae, Brachodidae, Brahmaeidae, Bucculatricidae, Carposinidae, Castniidae, Choreutidae, Cimeliidae, Coleophoridae, Copromorphidae, Cosmopterigidae, Cossidae, Crambidae, Dalceridae, Depressariidae, Douglasiidae, Drepanidae, Dryadaulidae, Elachistidae, Endromidae, Epermeniidae, Epipyropidae, Erebidae, Eriocottidae, Eriocraniidae, Ethmiidae, Euteliidae, Galacticidae, Gelechiidae, Geometridae, Glyphipterigidae, Gracillariidae, Heliodinidae, Heliozelidae, Hepialidae, Heterobathmiidae, Heterogynidae, Hyblaeidae, Incurvariidae, Lacturidae, Lasiocampidae, Lecithoceridae, Limacodidae, Lymantriidae, Lyonetiidae, Lypusidae, Meessiidae, Micropterigidae, Momphidae, Nepticulidae, Noctuidae, Nolidae, Notodontidae, Oecophoridae, Opostegidae, Parametriotidae, Peleopodidae, Plutellidae, Praydidae, Prodoxidae, Psychidae, Pterolonchidae, Pterophoridae, Pyralidae, Roeslerstammiidae, Animalia, Schreckensteiniidae, Scythrididae, Sesiidae, Sphingidae, Stathmopodidae, Thyrididae, Tineidae, Tischeriidae, Tortricidae, Uraniidae, Urodidae, Ustyurtiidae, Yponomeutidae, Ypsolophidae, Zygaenidae)",
 "Continent is Europe",
 "HasGeospatialIssue is false",
 "HasCoordinate is true"
 ]
}

 The dataset includes 72263771 records from 5462 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0051392-251120083545085/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-245114
title: Annual Holy Convocation of the Church of God in Christ (50th: 1957)

description: [description english/roman)] Jubilee program, golden anniversay holy convocation. The souvenir journal of the Holy Convocation of the Church of God in Christ. Annually.
DOI: 10.15468/dl.25fqfd
title: Occurrence Download

description: A dataset containing 485 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Platycephalus endrachtensis Quoy & Gaimard, 1825"
 ]
}

 The dataset includes 485 records from 41 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043728-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.c5bezm
title: Occurrence Download

description: A dataset containing 117 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Italy",
 "TaxonKey is Scolopax rusticola Linnaeus, 1758",
 "Year 2005-2020"
 ]
}

 The dataset includes 117 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0079660-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-245034
title: Annual Holy Convocation of the Church of God in Christ (40th: 1947)

description: [description english/roman)] The souvenir journal of the Holy Convocation of the Church of God in Christ. Annually.
DOI: 10.15468/dl.zyn2mq
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Senecio doryphoroides C.Jeffrey"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0123503-230530130749713/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-245135
title: Annual Holy Convocation of the Church of God in Christ (50th: 1957)

description: [description english/roman)] Jubilee program, golden anniversay holy convocation. The souvenir journal of the Holy Convocation of the Church of God in Christ. Annually.
DOI: 10.15468/dl.hnwj6n
title: Occurrence Download

description: A dataset containing 18 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Draba tolucensis Kunth"
 ]
}

 The dataset includes 18 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0043757-220831081235567/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ws9rjv
title: Occurrence Download

description: A dataset containing 15992 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Solanum pseudocapsicum L."
 ]
}

 The dataset includes 15992 records from 451 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0003258-240425142415019/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.scnkwe
title: Occurrence Download

description: A dataset containing 181 species occurrences available in GBIF matching the query:
{
 "and" : [
 {
 "or" : [
 "DatasetKey is iNaturalist Research-grade Observations",
 "DatasetKey is Natural History Museum Rotterdam - Specimens"
 ]
 },
 "TaxonKey is Melitaea parthenoides Keferstein, 1851"
 ]
}

 The dataset includes 181 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0019050-200613084148143/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.25549/pcra-c14-245010
title: The whole truth [magazine], 2009 February-April

description: [description english/roman)] The whole truth [magazine], 2009 February-April.