cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8195129.v1
title: Autoimmune Progesterone Dermatitis: A Case Report

description: Abstract  Introduction  Autoimmune progesterone dermatitis (APD) is a rare autoimmune dermatosis characterized by recurrent cutaneous and mucosal lesions during the luteal phase of the menstrual cycle that disappear some days after the menses.  Case Report A 34-year-old primipara woman with no significant past medical history and no prior exogenous hormone use, who presented with cyclic skin eruptions starting 1 year after the delivery. The lesions occurred 6 days before the menses and disappeared in between 1 and 2 days after the menstruation ceased. The patient was diagnosed after a positive response to an intradermal test with progesterone and was successfully treated with combined oral contraceptives. The skin eruptions have not returned since the initiation of this therapy.  Conclusion  Dermatologists, gynecologists, and obstetricians should be aware of this rare entity. Furthermore, if this condition is suspected, a thorough history taking on the menstrual cycle and results of the intradermal progesterone test are mandatory.
DOI: 10.6084/m9.figshare.8195132.v1
title: Simultaneous Determination of 2,3,7,8-TCDD and 2,3,7,8-TCDF in Water Samples by LLE-LTP and HPLC-DAD

description: Polychlorinated dibenzo-p-dioxins and dibenzofurans are persistent organic pollutants which can mainly be found in the environment in water. These compounds are highly toxic and therefore developing new extraction methodologies that are simpler and cost less is highly desirable. This study aimed to optimize and validate the liquid-liquid extraction with low-temperature partition methodology of 2,3,7,8-tetrachlorodibenzo-p-dioxin and 2,3,7,8-tetrachlorodibenzofuran in water samples. The optimum extraction conditions were 8.0 mL of the acetonitrile and 4.0 mL of the water, homogenized in a vortex for 1 min and then maintained at -20 ºC for 1 h. The analytes recovery percentage was close to 100% and relative standard deviation was less than 7%. The methodology was precise, accurate, linear at 2.4-5.4 µg L-1, and selective, with a quantification limit of 2.4 µg L-1. The optimized methodology was applied in ten real samples and the results showed that these analytes were not detected.
DOI: 10.6084/m9.figshare.8195018.v1
title: Correspondence between try-in pastes and resin cements, and color stability of bonded lithium disilicate disks

description: Abstract: This study investigates the color correspondence of resin cements and try-in pastes, and the color stability of bonded lithium disilicate ceramic disks. Resin composite disks were fabricated (n = 36) to serve as the background for lithium disilicate disks prepared in two thicknesses (0.5 and 1.0 mm, n = 18 each). Two brands were used for try-in and cement procedures: Variolink Veneer and AllCem Veneer. For baseline, water was applied between the ceramic disks and their respective backgrounds to achieve the control group. This set was subjected to color measurement using an intraoral measurement device (T0). The try-in was inserted between background and ceramic, and this set was subjected to color measurement (T1). After adhesive procedures, the ceramic disk was placed under cement, and color measurement was performed with uncured cement (T2) and 24 h after light-curing (T3). Each set was immersed in distilled water and thermal-cycled, with color measurement being performed after 10,000 (T4) and 20,000 (T5) cycles. Color differences were calculated by CIELab (rEab) and CIEDE2000 (rE00). Data were analyzed by two-way ANOVA for repeated measurements and Tukey's test (α=5%). There was color correspondence of try-in and resin cement for the Variolink system, regardless of the ceramic thickness (p &gt; 0.05). For the AllCem system, the thickness significantly influenced the color measurement (p &lt; 0.001). The Variolink system also demonstrated color stability after 20,000 thermal cycles with rEab &lt; 3.46 and rE00 &lt; 2.25. It was concluded that the color correspondence between a try-in and its respective cement may vary according to resin cement composition.
DOI: 10.6084/m9.figshare.8195036.v1
title: Adiposity indicators as a screening method for polysomnography in shift workers

description: Abstract  Objective: to verify the discriminatory power of adiposity indicators in the prediction of obstructive sleep apnoea (OSA) in shift workers.  Methods:  a cross-sectional study carried out in an iron ore extraction company, in Minas Gerais, Brazil. Anthropometric data were collected and polysomnography (PSG) was performed in 118 male shift workers who owned at least one overall risk factor for cardiovascular disease.  Results:  the OSA prevalence in the sample was 84.7%. Among the adiposity indicators used to predict OSA (≥ 5 events/hour), body mass index (BMI), waist circumference (WC), waist-to-height ratio (WHtR), and total body fat (TBF) showed sensitivity values higher than 70%. Visceral fat (VF), neck circumference (NC), and neck-to-height ratio (NHtR) were the most effective in correctly identifying workers without OSA (specificity values higher than 70%). The areas under the receiver operating characteristic (ROC) curves for WC and NHtR were greater than 0.7, which indicated the test was effective in discriminating individuals with OSA.  Conclusions:  alterations in abdomen and neck adiposity indicators have a significant relationship with the presence of OSA and showed effectiveness as a screening method for PSG. WC and NHtR are considered good indicators for OSA prediction.
DOI: 10.6084/m9.figshare.8195060.v1
title: Robot assisted radical prostatectomy in kidney transplant recipients: surgical, oncological and functional outcomes of two different robotic approaches

description: ABSTRACT  Background: To date, few series on robot-assisted radical prostatectomy (RARP) in kidney transplant recipients (KTRs) have been published.  Purpose: To report the experience of two referral centers adopting two different RARP approaches in KTRs. Surgical, oncological and functional results were primary outcomes evaluated in the study.  Material and methods: We retrospectively analyzed data from 9 KTRs who underwent transperitoneal RARP or Retzius-sparing RARP for PCa from October 2012 to April 2016. Data were reported as median and interquartile range (IQR). Pre- and postoperative outcomes were compared by non-parametric Wilcoxon signed-rank test. Significant differences were accepted when p ≤ 0.05. Overall survival was assessed using Kaplan-Meier method.  Results: Four KTRs underwent a T-RARP and 5 a RS-RARP. Patient median age was 60 (56-63) years. Charlson comorbidity index was 6 (5-6). Preoperative median PSA was 5.6 (5-15) ng / mL. Preoperative Gleason score (GS) was 6 in 5 patients, 7 (3 + 4) in 3, and 8 (4 + 4) in one. Pre- and postoperative creatinine were 1.17 (1.1; 1.4) and 1.3 (1.07; 1.57) mg / dL (p = 0.237), while eGFR was 66 (60-82) and 62 (54-81) mL / min / 1.73m2 (p = 0.553), respectively. One (11.1%) Clavien-Dindo grade II complication occurred. Two extended template lymphadenectomies were performed, both with nodal invasion. These two patients experienced a biochemical recurrence and were subjected to RT. Two patients (22.2%) had PSMs. Median follow-up was 42 months. Seven patients (77.8%) were continent, 5 (55.6%) were potent. Two (22.2%) patients died during follow-up for oncologic unrelated causes.  Conclusions: Our series suggests that both RARP approaches are safe and feasible techniques in KTRs for PCa.
DOI: 10.6084/m9.figshare.8195063.v1
title: A quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti-double-stranded DNA IgG antibodies

description: ABSTRACT  Introduction: The detection of anti-double-stranded (ds) deoxyribonucleic acid (DNA) antibodies is one of the classification criteria for diagnosing systemic lupus erythematosus (SLE).  Objective: To describe a quantitative enzyme-linked immunosorbent assay (ELISA) for detecting anti-dsDNA immunoglobulin class G (IgG) antibodies.  Methods: The performance of ELISA was evaluated using the Crithidia luciliae indirect immunofluorescence test (CLIFT) as a reference. Anti-dsDNA IgG antibodies were screened by ELISA and CLIFT in serum samples from 127 patients with SLE, 56 patients with other diseases and 37 healthy persons. The Cochran Q test was used to compare the sensitivity and specificity of the reactions, with differences among the results being considered significant when p ≤ 0.05.  Results: ELISA had a sensitivity of 92.9% and a specificity of 94.6%, whereas the sensitivity and specificity of CLIFT were 85.8% and 100%, respectively. ELISA was significantly more sensitive than CLIFT (p = 0.0027), whereas CLIFT was significantly more specific than ELISA (p = 0.0253).  Conclusion: ELISA showed excellent results in terms of sensitivity and specificity, with a potential use in research and routine diagnostics.
DOI: 10.6084/m9.figshare.8195066
title: Elective Educational Unit: International Exchange Experience in Undergraduate Medical Training

description: ABSTRACT As part of the integrated curriculum, the Elective Educational Unity (EEU) is a tool to support knowledge construction and student autonomy during undergraduate training. The international exchange for undergraduates therefore aims to explore potentialities and challenges within a pedagogical perspective through the experience and understanding ofdiverse educational institutions and health services, through prolonged contact with other professionals and students. One constructive strategy of an elective internship is in conjunction with student associations. This report intends to contribute to medical education by reflecting on how the international elective internship impacts on medical training, analysing its potentialities and challenges. The reportaddresses the steps in accomplishing the exchange, presenting the activities developed in the experience, highlightingthe observed objectives and results, along with reflections on the experience. It is ascertained that the international exchange stimulates the experience, recognition and understanding of other cultures; it helps students build autonomy and stimulates a critical reflexive view which promotes knowledge construction, raising the student’s awareness of his own social responsibility as a health professional. Therefore, the Elective Educational Unit encourages the student to elect the exchange as a curricular activity, it supports him on choosing a study area and international or national institution of interest, with the option to liaise with other organizations, for example the student organization. Unquestionably, personal growth is one of the consequences through learning about diversecultures and making new friendships and partnerships between different countries. However, there are challenges, such as adaptation to another cultural context, affective detachment and the understanding that different places have distinct historical, social, economic contexts from one another. Thus, advances and changes are proposed to improve the Elective Educational Unit, stimulating greater student participation in international experiences during undergraduate training, as well as underlining the importance of the supervisor’s role in the elective planning process.
DOI: 10.6084/m9.figshare.8195075
title: Growth, antioxidant system, and immunological status of shrimp in bioflocs and clear water culture systems

description: Abstract: The objective of this work was to evaluate the effect of the traditional culture system in clear water and of the one in microbial flocs on the antioxidant and immunological status of Litopenaeus vannamei shrimp. Samples (gill, hemolymph, and hepatopancreas) were collected on days 15, 30, and 60 of the experimental period. The following immunological parameters were analyzed at each evaluation time: granular and hyaline hemocytes, total protein, and apoptosis. Assays on glutamate-cysteine ligase (GCL) activity and on the concentrations of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were also performed. The evaluated immunological parameters did not differ significantly between treatments. Shrimp reared in clear water showed higher levels of lipid peroxidation in the gills and of GCL activity in the hemolymph on days 15 and 30. Shrimp in microbial floc had a higher survival rate, and the water did not have to be renewed during the experimental period. The microbial floc system improves production levels and provides a healthier culture environment.
DOI: 10.6084/m9.figshare.8195090
title: Effect of extrusion cooking on physicochemical properties of tuna meat-based extrudates

description: Abstract Effect of extrusion cooking using different corn starch contents on the physicochemical properties (expansion ratio, bulk density, color, hardness, protein in vitro digestibility, crude protein content, thermal properties and microstructure) of tuna meat-based extrudates was investigated. The results showed that the increased corn starch content led to darker, greener, yellower and lower crude protein content of extrudates, but no significant difference was observed in expansion ratio, bulk density and hardness. The lowest protein in vitro digestibility of 82.83±0.39% appeared when the corn starch content was 20 wt%. The results of DSC revealed that tuna meat with corn starch content of 30 wt% and 20 wt% had the highest and lowest denaturation temperature of 99.4oC and 81.9 oC, respectively. The results of SEM indicated that the extrudate with 30 wt% corn starch content possessed the more integrated and distinct muscle fibers. Besides, the acceptability of the extrudate with 30 wt% corn starch content was significant better than others (p˂0.05). These results revealed that the tuna meat-based extrudates using 30 wt% of corn starch exhibited desirable physicochemical properties.
DOI: 10.6084/m9.figshare.8195090.v1
title: Effect of extrusion cooking on physicochemical properties of tuna meat-based extrudates

description: Abstract Effect of extrusion cooking using different corn starch contents on the physicochemical properties (expansion ratio, bulk density, color, hardness, protein in vitro digestibility, crude protein content, thermal properties and microstructure) of tuna meat-based extrudates was investigated. The results showed that the increased corn starch content led to darker, greener, yellower and lower crude protein content of extrudates, but no significant difference was observed in expansion ratio, bulk density and hardness. The lowest protein in vitro digestibility of 82.83±0.39% appeared when the corn starch content was 20 wt%. The results of DSC revealed that tuna meat with corn starch content of 30 wt% and 20 wt% had the highest and lowest denaturation temperature of 99.4oC and 81.9 oC, respectively. The results of SEM indicated that the extrudate with 30 wt% corn starch content possessed the more integrated and distinct muscle fibers. Besides, the acceptability of the extrudate with 30 wt% corn starch content was significant better than others (p˂0.05). These results revealed that the tuna meat-based extrudates using 30 wt% of corn starch exhibited desirable physicochemical properties.
DOI: 10.6084/m9.figshare.8195105
title: Hyperuricemia in Down syndrome children and adolescents

description: ABSTRACT  Introduction: Patients with Down syndrome (DS) seem to have higher rates of hyperuricemia, although there are very few studies that address this issue. High levels of uric acid (UA) are an independent risk factor for atherosclerotic heart disease.  Objective: To study the prevalence of hyperuricemia in DS children and its distribution according to age.  Methods: Retrospective study of 139 DS patients aged 1-14 years for serum UA values considering the normal cut-off values for age.  Results: Among the 139 included patients, 70 (50.4%) were males, 69 (49.6%) were females, and the mean age was 5.31 ± 3.49 years (range 1-14 years). In this sample, 39 (28.1%) had serum UA levels above the normal value. We did not find differences in the serum UA according to gender, except in patients in the range of 13-15 years, where males had mean UA levels of 5.75 ± 1.37 mg/dl; and females, of 8.02 ± 0.77 mg/dl (p = 0.02).  Conclusion: We found a high rate of hyperuricemia in children with DS that equally affects both genders, except in the age range of 13-15 years, in which it was more common among females.
DOI: 10.6084/m9.figshare.8195105.v1
title: Hyperuricemia in Down syndrome children and adolescents

description: ABSTRACT  Introduction: Patients with Down syndrome (DS) seem to have higher rates of hyperuricemia, although there are very few studies that address this issue. High levels of uric acid (UA) are an independent risk factor for atherosclerotic heart disease.  Objective: To study the prevalence of hyperuricemia in DS children and its distribution according to age.  Methods: Retrospective study of 139 DS patients aged 1-14 years for serum UA values considering the normal cut-off values for age.  Results: Among the 139 included patients, 70 (50.4%) were males, 69 (49.6%) were females, and the mean age was 5.31 ± 3.49 years (range 1-14 years). In this sample, 39 (28.1%) had serum UA levels above the normal value. We did not find differences in the serum UA according to gender, except in patients in the range of 13-15 years, where males had mean UA levels of 5.75 ± 1.37 mg/dl; and females, of 8.02 ± 0.77 mg/dl (p = 0.02).  Conclusion: We found a high rate of hyperuricemia in children with DS that equally affects both genders, except in the age range of 13-15 years, in which it was more common among females.
DOI: 10.6084/m9.figshare.8195108.v1
title: Diagnostic potential of saliva proteome analysis: a review and guide to clinical practice

description: Abstract: Proteomic techniques have become popular in medicine and dentistry because of their widespread use in analyzing bodily fluids such as blood, saliva, urine, and gingival crevicular fluids as well as hard tissues such as enamel, dentine, and cementum. This review is a guide to proteomic techniques in general dentistry, summarizing techniques and their clinical application in understanding and diagnosing diseases and their use in identifying biomarkers of various diseases.
DOI: 10.6084/m9.figshare.8195111
title: Semiconductor Nanocrystals-Reduced Graphene Composites for the Electrochemical Detection of Carbendazim

description: A new nanocomposite based on ZnCdTe semiconductor nanocrystals (NCs) synthesized in situ on reduced graphene oxide (rGO) was obtained. The heterostructure was characterized using UV-Vis emission and absorption spectroscopies, which provided evidences of the growth of the nanocrystals onto the rGO matrix. To evaluate the electrochemical performance, carbon paste electrodes modified with the nanocomposite (QD-rGO/CPE) were prepared, showing high sensitivity in the electroanalytical detection of the pesticide carbendazim. Under optimal operational conditions a calibration curve was constructed with a linear behavior in the range of 9.98 × 10-8 to 1.18 × 10-5 mol L-1, with a correlation coefficient of 0.997. The limit of detection (LOD) and the limit of quantification (LOQ) for carbendazim were 9.16 × 10-8 and 2.78 × 10-7 mol L-1, respectively. The electrode is successfully applied for the determination of carbendazim in orange juice samples.
DOI: 10.6084/m9.figshare.8195114
title: First-year students’ perceptions of team-based learning in a new medical genetics course

description: ABSTRACT  Background  Medical education has evolved considerably over the last few years, especially through adoption of new technologies and active methodologies. These methodologies aim to improve learning and engage students deeply in the process. TBL is a methodology widely used in health schools, including Medical Schools. We can use it to work with large groups, divided into small teams. The students first work individually, then within teams, and finally the groups cooperate to solve applied problems.  Objectives  To describe students’ perceptions and satisfaction about a Medical Genetics course organized into blocks of subject in which we used TBL sessions with first-year medical students.  Methods  A Medical Genetics course were organized into subject blocks in which a TBL session was conducted in each of these blocks to improve the learning process. At the end of the course, the students answered a questionnaire on satisfaction and perceptions.  Results  By the first time we described a Medical Genetics course organized into 5 blocks of subject matter on a total of 25 genetic diseases in which a TBL session was conducted in each of these blocks. We enrolled a total of 290 participants and 96% of the students were satisfied with TBL. Furthermore, 97% of students believe that TBL helped them to learn, and 87% approved of use of TBL in the future at other stages of their medical course.  Conclusion  Application of the TBL method during a medical genetics course was well-received by students and proved an important tool in the structures of curricula for medical education at this university.
DOI: 10.6084/m9.figshare.8195117
title: Coccidian parasites from birds at rehabilitation centers in Portugal, with notes on Avispora bubonis in Old World

description: Abstract Portugal has some rehabilitation centers for wild animals, which are responsible for the rehabilitation and reintroduction of birds, among other animals, into the wild. Coccidian parasites of these wild birds in rehabilitation centers are especially important because these centers can introduce coccidian species into new environments through the reintroduction of their respective hosts. In this context, the current study aimed to identify intestinal coccidia from wild birds at two rehabilitation centers for wild animals located in two municipalities of Portugal. Eighty-nine wild birds of 9 orders and 11 families were sampled, of which 22 (25%) were positive for Coccidia. Avispora spp. were found in raptors. Sporocysts of Sarcocystinae subfamily were recovered from owls. An Isospora sp. was found in Turdus merula Linnaeus, 1758, and an Eimeria sp. was found in Fulica atra Linnaeus, 1758. Among the coccidian species, Avispora bubonis (Cawthorn, Stockdale, 1981) can be highlighted. The finding of this species indicates that transmission of coccidians from the New World to the Old World may be occurring, potentially through dispersion by Bubo scandiacus (Linnaeus, 1758) through Arctic regions or by means of anthropic activities, and/or through other unknown mechanisms.