DOI: 10.20350/digitalcsic/18112
title: Opuntia extract-supplemented and control (estabilization and wash-out periods) microbiota [DataSet]

description: Faecal microbiota from a healthy adult donor was inoculated into the three colon reactors (AC: acending colon, TC: transverse colon, and DC: desending colon) of the BFBL gut simulator. An initial 7-day stabilization period of the microbial community was performed to reach the steady state prior to the extract supplementation. After this stabilization period, 1g of Opuntia extract was added each day (for 7 days) supplemented to the simulator at the ‘breakfast’ stage. Finally, another 7-day wash-out period was included. This complete process was performed three times, as extracts from three different Opuntia varieties were tested: Opuntia stricta var. dillenii (OD), Opuntia ficus-indica var. Colorada pulp extract (COL), and Opuntia ficus-indica var. Blanca peel extract (BB). It was performed sequentially and each assay included independent stabilization and wash-out phases between treatments. During the experimental set up, samples were collected every 24 h from the three colon reactors (AC, TC, and DC) and centrifuged (10,000 ×g during 10 min at 4 °C), and the pellet was used for bacterial genomic DNA extraction (EZNA® Bacterial DNA Kit, Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). The extracted DNA underwent amplicon-based metagenomic sequencing targeting the full-length 16S rRNA gene that was sequenced by Novogene (Munich, Germany) using the PacBio Sequel II/IIe platform.
description: In all files, colonic reactors are labelled as R1, R2, R3, for AC, TC and DC, respectively. In the files corresponding to the assay performed with O. dillenii whole fruit extract (OD), T4, T5, and T6, refer to the three replicates analyzed in the stabilization period before supplementation with OD (labelled as STAB in the article). T18, T20 and T22 refer to the three replicates analyzed during supplementation of the simulator with OD. T28, T29, and T30 refer to the three replicates analyzed in the wash-out period after supplementation with OD. In the files corresponding to the assay performed with O. ficus-indica var. Colorada pulp extract (COL) and O. ficus-indica var. Blanca peel extract (BB), T5, T6, and T7, refer to the three replicates analyzed in the stabilization period before supplementation with COL (labelled as STAB in the article). T11, T13 and T14 refer to the three replicates analyzed during supplementation of the simulator with COL. T17, T18, and T19 refer to the three replicates analyzed in the wash-out period after supplementation with COL, but also to the stabilization period before supplementation with BB. T24, T25 and T26 refer to the three replicates analyzed during supplementation of the simulator with BB. T29, T30, and T31 refer to the three replicates analyzed in the wash-out period after supplementation with BB.
description: In all files, colonic reactors are labelled as R1, R2, R3, while in the manuscript as AC, TC and DC, respectively. In the files corresponding to the assay performed with O. dillenii whole fruit extract (OD), T4, T5, and T6, refer to the three replicates analyzed in the stabilization period before supplementation with OD (labelled as STAB in the manuscript). T18, T20 and T22 refer to the three replicates analyzed during supplementation of the simulator with OD. T28, T29, and T30 refer to the three replicates analyzed in the wash-out period after supplementation with OD (labelled as W/O in the manuscript). In the files corresponding to the assay performed with O. ficus-indica var. Colorada pulp extract (COL) and O. ficus-indica var. Blanca peel extract (BB), T5, T6, and T7, refer to the three replicates analyzed in the stabilization period before supplementation with COL (labelled as STAB in the manuscript). T11, T13 and T14 refer to the three replicates analyzed during supplementation of the simulator with COL. T17, T18, and T19 refer to the three replicates analyzed in the wash-out period after supplementation with COL (labelled as W/O in the manuscript), but also to the stabilization period before supplementation with BB (labelled as STAB in the manuscript). T24, T25 and T26 refer to the three replicates analyzed during supplementation of the simulator with BB. T29, T30, and T31 refer to the three replicates analyzed in the wash-out period after supplementation with BB (labelled as W/O in the manuscript).