cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.m5r8fj
title: Occurrence Download

description: A dataset containing 114357 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Euphausia"
 ]
}

 The dataset includes 114357 records from 187 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046636-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.10490.88006
title: Could I Possibly and Technically be 116% Western European(Celtic and Germanic) Due to Mutations? P1
DOI: 10.13140/rg.2.2.28369.10089/1
title: Address of my article "THE AGE OF MORAL ARIDITY AND INSENSITIVITY" - Polis Magazino - https://www.polismagazino.gr/article-by-gianluigi-segalerba/?fbclid=IwAR2bfJuZHEUT37XS3a0rHOMS4Mg6lIHgghB7ZYxTriXrdt4-58o2sIj-pt0
DOI: 10.13140/rg.2.2.21605.78566
title: LAPORAN ANGKET PENGARUH PERKEMBANGAN MEDIA SOSIAL PADA KEHIDUPAN REMAJA SISWA KELAS 12 MIPA 2 SMAN 9 MALANG
DOI: 10.15468/dl.amjedm
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((-165 -30,-157.5 -30,-157.5 -22.5,-165 -22.5,-165 -30))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046701-251009101135966/datasets/export for details.
DOI: 10.13140/rg.2.2.11565.67041
title: Kenya et Haïti : un résumé comparatif des deux pays selon les données
DOI: 10.15468/dl.97h4au
title: Occurrence Download

description: A dataset containing 1754 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((142.5 30,150 30,150 37.5,142.5 37.5,142.5 30))"
 ]
}

 The dataset includes 1754 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046702-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.28257.68963
title: Screenshot 20230801 111956 ResearchGate
DOI: 10.13140/rg.2.2.31927.70566
title: Geology map of Himalaya By Wang Jia-Min et al. 2022-AGS, CorelDraw file that could be edited and modified
DOI: 10.13140/rg.2.2.18741.86245
title: Action Research - To find out and solve the problems faced by students of class V in learning basic skills of Marathi language, reading,writing and speaking .عملی تحقیق-پنجم جماعت کے طلبہ کو مراٹھی زبان کی ابتدائی مہارتیں بولنے،لکھنے،پڑھنے میں درپیش مسائل معلوم کرنا اور اسکا حل
DOI: 10.13140/rg.2.2.16841.31840
title: fashion wicked problems
DOI: 10.13140/rg.2.2.28795.08485
title: Indikator Kesejahteraan Rakyat Infrastruktur Dasar (Buku IV)
DOI: 10.13140/rg.2.2.25439.64166
title: 2010 Indikator Kesejahteraan Rakyat Kemiskinan (Buku I)
DOI: 10.13140/rg.2.2.12175.15527
title: IQ Privilege Theorem
DOI: 10.13140/rg.2.2.35007.89761
title: The mammalian T cell receptor gamma variable (TRGV) subgroups IMGT-NC Report 20/07/23
DOI: 10.13140/rg.2.2.15766.52806
title: Botânica Simplificada - Aplicativo
DOI: 10.13140/rg.2.2.13356.03203
title: Political history of Pakistan
DOI: 10.13140/rg.2.2.29923.53283
title: EAVA. Cardio Go Conference 2023 August 10-11. Integrated case scenarios. Samir Rafla-Agenda
DOI: 10.15468/dl.emfzj2
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((-172.5 -30,-165 -30,-165 -22.5,-172.5 -22.5,-172.5 -30))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046703-251009101135966/datasets/export for details.
DOI: 10.15468/dl.n8uxxe
title: Occurrence Download

description: A dataset containing 1223 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((150 30,157.5 30,157.5 37.5,150 37.5,150 30))"
 ]
}

 The dataset includes 1223 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046705-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.13152.87045
title: Análise matemática e estatística do grau de descontaminação de água via radiação ultravioleta
DOI: 10.13140/rg.2.2.34438.96324
title: Gd2W3O12:Sm(1%) Optical Data and Spectra
DOI: 10.15468/dl.yx2f9h
title: Occurrence Download

description: A dataset containing 1252 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((157.5 30,165 30,165 37.5,157.5 37.5,157.5 30))"
 ]
}

 The dataset includes 1252 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046706-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.13205.29923
title: triplot-compare-RGB
DOI: 10.15468/dl.7dhks9
title: Occurrence Download

description: A dataset containing 5 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((-180 -30,-172.5 -30,-172.5 -22.5,-180 -22.5,-180 -30))"
 ]
}

 The dataset includes 5 records from 3 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046707-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.19575.39845
title: (unfinished draft ~ 2015) Application Holy Wars or a New Reformation? - A fugue on the theory of knowledge
DOI: 10.13140/rg.2.2.17006.38722/1
title: Haïti : Classement des meilleures universités pour 2023
DOI: 10.13140/rg.2.2.15852.95362
title: 1:5M-International-Geological-Map-of-Asia
DOI: 10.13140/rg.2.2.26338.71367
title: Certificate of a peer reviewer for the International research journal
DOI: 10.13140/rg.2.2.28226.15046
title: Project Based Learning 2022-23 Abstractive QF-MDS Natural Language Processing.pptx
DOI: 10.13140/rg.2.2.30152.90880
title: Major William Alexander Brown,MBE,Sitara e Pakistan-British Scot Leader of Gilgit Rebellion
DOI: 10.13140/rg.2.2.35985.61289
title: Combining Full Spectrum Light with Color and Sound for Holistic Treatment COMBINING FULL SPECTRUM LIGHT WITH COLOR AND SOUND 2
DOI: 10.15468/dl.yqk2ca
title: Occurrence Download

description: A dataset containing 890 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Machine Observation, Observation)",
 "Continent is Europe",
 "OccurrenceStatus is Present",
 "TaxonKey is Mustela lutreola",
 "Year 2000-2025"
 ]
}

 The dataset includes 890 records from 53 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046708-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.10780.46721
title: Regulating legal repercussions of doubt in disinformation leading to defamation, liability of research affiliates in misinformation legislation in the UK…
DOI: 10.13140/rg.2.2.25617.81763
title: a master of the art of subtle exaggerations
DOI: 10.13140/rg.2.2.14607.76960
title: JMESTN42352459.pdf
DOI: 10.13140/rg.2.2.35431.83360
title: MANUAL BÁSICO METODOLÓGICO DE PRODUCCIÓN DE MANGLES EN VIVERO 2009
DOI: 10.13140/rg.2.2.35405.61921
title: Paper Solution MCA -III Semester MCA-301 Dec-2015 RGPV Bhopal MP
DOI: 10.13140/rg.2.2.14748.67207
title: 16 Divison is cut into two parts
DOI: 10.13140/rg.2.2.21878.98887
title: Mapa Geológico da Área de Cabeça Veada, Maciço Calcário Estremenho (1:5000)
DOI: 10.13140/rg.2.2.12599.09128
title: 979 Tournament 11 May 2021 ChessBase Playchess 2 place FIDE Master Tihomir Dovramadjiev with username Lacetti
DOI: 10.13140/rg.2.2.29166.59206
title: Pedagogical Social Scientific Applied Mathematical Paradoxical Theorem ("PSSAMPT")
DOI: 10.15468/dl.wmkmmc
title: Occurrence Download

description: A dataset containing 1931 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((172.5 -22.5,180 -22.5,180 -15,172.5 -15,172.5 -22.5))"
 ]
}

 The dataset includes 1931 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046709-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.21511.98720
title: Sustainable Transport Hierarchy and Active Mobility
DOI: 10.13140/rg.2.2.15993.85600
title: 2016-07-19-1-G TRR 1
DOI: 10.6084/m9.figshare.30405283
title: Supplementary Material for: Clinical description of ten pediatric patients with Rapid-onset obesity and clinical signs of ROHHAD-NET Syndrome and review of the literature

description: Introduction: Rapid-onset obesity with hypothalamic dysfunction, hypoventilation, and autonomic dysregulation (ROHHAD) syndrome is a rare disorder characterized by respiratory failure, autonomic dysregulation, and endocrine abnormalities. Some of these patients may also develop neuroendocrine tumors (ROHHAD-NET). Aim: To evaluate the clinical features, biochemical findings, treatments and outcomes in a cohort of pediatrics patients with ROHHAD-NET syndrome followed at a single tertiary center. Subjects and Methods: Retrospective review of 10 medical records of patients with ROHHAD-NET syndrome followed between 2007-2024. Data analyzed included clinical, biochemical, therapeutic interventions and outcomes. Results: Rapid onset obesity, observed all 10 patients, was the first clinical sign with a mean age at onset (MAO) of 5.1 ± 1.8 years. Neurobehavioral disorders were also an early manifestation, occurring in 5 cases with a MAO of 5 ± 1 years. All patients developed respiratory symptoms, with a MAO of 7.4 ± 3 years. Six patients were diagnosed with obstructive sleep apnea syndrome, including 2 with associated alveolar central hypoventilation. Seven patients required ventilatory support. All patients had hypothalamic dysfunction, MAO 6.6 ± 1.3. Disorders of water balance were the most frequent manifestation (n: 10), followed by hyperprolactinemia, central hypothyroidism, growth hormone deficiency, adrenal insufficiency, precocious puberty, and hypogonadotropic hypogonadism. Autonomic dysregulation was present in the entire cohort (MAO 6.7 ± 2.3), with strabismus as the most common clinical sign (n: 6). Neural crest tumors (ganglioneuromas) were identified in 5 patients, with a MAO of 5.4 ± 1.3 years. Tumor location included the adrenal gland (n: 2) and mediastinum (n = 3). Other clinical findings included thrombocytopenia, celiac disease, enuresis, type 1 and type 2 diabetes, seizures, hypo- and hypertension, and primary hypothyroidism. All patients received symptomatic treatment; 3 also received intravenous immunoglobulin as compassionate therapy, without significant benefit. Three patients died. Conclusion: High clinical suspicion and early recognition are essential for a prompt diagnosis, timely initiation of respiratory support, and reduction of morbidity and mortality. Larger cohorts are needed for better characterization of this syndrome and to further elucidate its etiology.
DOI: 10.6084/m9.figshare.30405283.v1
title: Supplementary Material for: Clinical description of ten pediatric patients with Rapid-onset obesity and clinical signs of ROHHAD-NET Syndrome and review of the literature

description: Introduction: Rapid-onset obesity with hypothalamic dysfunction, hypoventilation, and autonomic dysregulation (ROHHAD) syndrome is a rare disorder characterized by respiratory failure, autonomic dysregulation, and endocrine abnormalities. Some of these patients may also develop neuroendocrine tumors (ROHHAD-NET). Aim: To evaluate the clinical features, biochemical findings, treatments and outcomes in a cohort of pediatrics patients with ROHHAD-NET syndrome followed at a single tertiary center. Subjects and Methods: Retrospective review of 10 medical records of patients with ROHHAD-NET syndrome followed between 2007-2024. Data analyzed included clinical, biochemical, therapeutic interventions and outcomes. Results: Rapid onset obesity, observed all 10 patients, was the first clinical sign with a mean age at onset (MAO) of 5.1 ± 1.8 years. Neurobehavioral disorders were also an early manifestation, occurring in 5 cases with a MAO of 5 ± 1 years. All patients developed respiratory symptoms, with a MAO of 7.4 ± 3 years. Six patients were diagnosed with obstructive sleep apnea syndrome, including 2 with associated alveolar central hypoventilation. Seven patients required ventilatory support. All patients had hypothalamic dysfunction, MAO 6.6 ± 1.3. Disorders of water balance were the most frequent manifestation (n: 10), followed by hyperprolactinemia, central hypothyroidism, growth hormone deficiency, adrenal insufficiency, precocious puberty, and hypogonadotropic hypogonadism. Autonomic dysregulation was present in the entire cohort (MAO 6.7 ± 2.3), with strabismus as the most common clinical sign (n: 6). Neural crest tumors (ganglioneuromas) were identified in 5 patients, with a MAO of 5.4 ± 1.3 years. Tumor location included the adrenal gland (n: 2) and mediastinum (n = 3). Other clinical findings included thrombocytopenia, celiac disease, enuresis, type 1 and type 2 diabetes, seizures, hypo- and hypertension, and primary hypothyroidism. All patients received symptomatic treatment; 3 also received intravenous immunoglobulin as compassionate therapy, without significant benefit. Three patients died. Conclusion: High clinical suspicion and early recognition are essential for a prompt diagnosis, timely initiation of respiratory support, and reduction of morbidity and mortality. Larger cohorts are needed for better characterization of this syndrome and to further elucidate its etiology.
DOI: 10.6084/m9.figshare.30405289
title: Supplementary Material for: Effect of reduced dialysate flow on dialysis adequacy: a pilot study

description: The standard dialysate flow (Qd) for hemodialysis (HD) is currently set at 500 mL/min. One potential, sustainable and cost-effective solution for eco-friendly HD may involve reducing Qd to limit waterwaste. However, the effect of reduced Qd on small and middle molecules (MM) removal remains to be investigated.
In this prospective observational study,74 patients on different maintenance dialysis modalities with Qd set at 500 mL/min (Qd500), were assigned to receive Qd 400 mL/min (Qd400) for 3 months. Dialysis adequacy including small solutes removal and MM reduction ratio (RR) was evaluated at enrolment and after 3 months. 
Compared to Qd500, Qd400 after 3 months achieved similar spKt/V (1.41±0.30 vs 1.43±0.33, p=0.58), eKt/V, urea RR, creatinine RR and phosphate RR.
Qd400 vs Qd500 provided significantly higher beta2-microglobulin RR [77.0 (71.4-81.7) vs 74.7 (68.4-79.4) %, p=0.009] and lower kappa FLC RR [54.2 (42.1-64.4) vs 57.6 (41.6-65.0) %, p=0.03], whereas myoglobin and lambda FLC RR were similar.
Qd400 resulted in higher pre-dialysis urea (20.2±5.5 vs 18.2±6.2 mmol/L, p=0.002), creatinine (694.0±179.5 vs 665.6±220.4 µmol/L, p=0.029), beta2-microglobulin [26.5 (23.0-30.0) vs 23.5 (20.0-28.0) mg/L, p=0.0001] and myoglobin [174.0 (122.0-251.0) vs 159.5 (119.0-195.0) µg/l, p=0.033] levels. Pre-dialysis levels of albumin, potassium, bicarbonate, phosphate and calcium were similar between Qd400 and Qd500.
Three months of Qd at 400 mL/min appears to provide similar small and MM removal, but with an increase in pre-dialysis urea, creatinine, beta2-microglobulin and myoglobin levels. Although this strategy could help preserving water, its potential impact on long-term clinical outcomes deserves further evaluation.
DOI: 10.6084/m9.figshare.30405289.v1
title: Supplementary Material for: Effect of reduced dialysate flow on dialysis adequacy: a pilot study

description: The standard dialysate flow (Qd) for hemodialysis (HD) is currently set at 500 mL/min. One potential, sustainable and cost-effective solution for eco-friendly HD may involve reducing Qd to limit waterwaste. However, the effect of reduced Qd on small and middle molecules (MM) removal remains to be investigated.
In this prospective observational study,74 patients on different maintenance dialysis modalities with Qd set at 500 mL/min (Qd500), were assigned to receive Qd 400 mL/min (Qd400) for 3 months. Dialysis adequacy including small solutes removal and MM reduction ratio (RR) was evaluated at enrolment and after 3 months. 
Compared to Qd500, Qd400 after 3 months achieved similar spKt/V (1.41±0.30 vs 1.43±0.33, p=0.58), eKt/V, urea RR, creatinine RR and phosphate RR.
Qd400 vs Qd500 provided significantly higher beta2-microglobulin RR [77.0 (71.4-81.7) vs 74.7 (68.4-79.4) %, p=0.009] and lower kappa FLC RR [54.2 (42.1-64.4) vs 57.6 (41.6-65.0) %, p=0.03], whereas myoglobin and lambda FLC RR were similar.
Qd400 resulted in higher pre-dialysis urea (20.2±5.5 vs 18.2±6.2 mmol/L, p=0.002), creatinine (694.0±179.5 vs 665.6±220.4 µmol/L, p=0.029), beta2-microglobulin [26.5 (23.0-30.0) vs 23.5 (20.0-28.0) mg/L, p=0.0001] and myoglobin [174.0 (122.0-251.0) vs 159.5 (119.0-195.0) µg/l, p=0.033] levels. Pre-dialysis levels of albumin, potassium, bicarbonate, phosphate and calcium were similar between Qd400 and Qd500.
Three months of Qd at 400 mL/min appears to provide similar small and MM removal, but with an increase in pre-dialysis urea, creatinine, beta2-microglobulin and myoglobin levels. Although this strategy could help preserving water, its potential impact on long-term clinical outcomes deserves further evaluation.
DOI: 10.13140/rg.2.2.13293.77284
title: Presentation - THE FUTURE OF WORK IN AN AI-DOMINATED WORLD: MAINTAINING THE VALUE OF HUMAN SKILLS THROUGH EMOTIONAL INTELLIGENCE DEVELOPMENT IN CHILDREN
DOI: 10.13140/rg.2.2.22101.81128
title: GM Yuri Solodovnichenko (UKR) 2519 0:1 FM Tihomir Dovramadjiev (BUL) 2332, official tournament game, 25 June 2005
DOI: 10.13140/rg.2.2.21420.23688
title: IM Nikolai Looshnikov (RUS) 2437 0:1 FM Tihomir Dovramadjiev (BUL) 2342, official tournament game, 6 May 2006
DOI: 10.13140/rg.2.2.25614.54081
title: FM Tihomir Dovramadjiev (BUL) 2331 1/2:1/2 GM Hikaru Nakamura (USA) 2571, official tournament game, 4 May 2004
DOI: 10.13140/rg.2.2.21105.66401
title: GM David Baramidze (GER) 2554 0:1 FM Tihomir Dovramadjiev (BUL) 2342, official tournament game, 22 January 2006
DOI: 10.15468/dl.ghb8hr
title: Occurrence Download

description: A dataset containing 2650 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Sphenodontia, Crocodylia, Squamata, Testudines)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1980",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen)"
 ]
 }
 },
 "Geometry POLYGON((165 30,172.5 30,172.5 37.5,165 37.5,165 30))"
 ]
}

 The dataset includes 2650 records from 5 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0046710-251009101135966/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.13140/rg.2.2.10410.18885
title: Sertifikat Reviewer D.I. Ansusa_Jurnal Cogent Art and Humanities_Scopus Q2
DOI: 10.13140/rg.2.2.18608.74246
title: FM Tihomir Dovramadjiev (BUL) 2342 1:0 GM Kirill Bryzgalin (RUS) 2474, official tournament game, Round "1.3", 21 January 2006
DOI: 10.13140/rg.2.2.11583.28323
title: XCS-RC-Visualization-Tool-06-The-Maze-Environment
DOI: 10.13140/rg.2.2.35018.95681
title: الروابط اللغوية واللفظية في الكتابة الاكاديمية ورقة ١
DOI: 10.6084/m9.figshare.30405292
title: Supplementary Material for: Subtype- and Layer-Specific Developmental Gradients of Postnatal GABAergic Neurons in the Rodent Barrel Cortex

description: Introduction: The developing brain exhibits rostro-caudal gradients that align with the maturation of functionally organized circuits. The barrel cortex, a spatially precise sensory structure, serves as an ideal model to examine such gradients within a confined functional domain. GABAergic interneurons, characterized by subtype-specific developmental trajectories and pivotal roles in early cortical dynamics, provide a strategic cellular entry point for this investigation.

Methods: To explore cellular and functional gradients in the developing barrel cortex, we combined transgenic mouse lines, immunohistochemical analyses with in vivo electrophysiological recordings of whisker-evoked activity during early postnatal stages. We also employed a model of ethanol exposure to assess potential differences in apoptotic vulnerability along the rostro-caudal axis.

Results: Immunohistochemistry revealed distinct layer- and subtype-specific gradients of GABAergic neurons. Notably, we observed a widespread rostro-caudal gradient in 5-HT3AR-expressing cells and a localized gradient of somatostatin-positive (SST+) interneurons in the deep layers. These gradients diminished in a subtype-specific manner from postnatal day (P)5 to P10, indicating transient developmental features. In vivo recordings showed that caudal whisker stimulation elicited stronger responses, while rostral stimulation produced a broader spatial spread of activity, suggesting region-specific functional properties. Furthermore, rostral regions exhibited higher expression of the maturation marker KCC2, supporting the notion of more advanced maturation in the rostral barrel cortex. Ethanol exposure induced greater apoptosis in caudal layer 5 compared to its rostral counterpart, revealing layer- and region-specific vulnerabilities.

Conclusions: These findings highlight spatially regulated trajectories of cortical maturation and underscore how regional differences in development may influence sensory processing and contribute to the heterogeneity of symptoms observed in neurodevelopmental disorders.