DOI: 10.5061/dryad.fbg79cnxn
title: Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females

description: Sexual activity (mating) negatively affects immune function in various
 insect species, in both sexes. In the experiments reported in this
 manuscript, we tested if hosts adapted to regular pathogen challenges are
 less susceptible to mating-induced immune suppression, using
 experimentally evolved Drosophila melanogaster populations
 selected for increased post-infection survival when infected with a
 Gram-positive bacterium, Enterococcus faecalis. Mating increased
 the susceptibility of females to bacterial pathogens but in a
 pathogen-specific manner. Mating-induced increase in susceptibility was
 also affected by host evolutionary history, with females from selected
 populations exhibiting similar post-infection survival irrespective of
 mating status, while females from control populations became more
 susceptible to bacterial infections after mating. Post-infection survival
 of males, irrespective of their evolutionary history, was not affected by
 their mating status. We, therefore, conclude that hosts evolved to better
 survive bacterial infections are also better at resisting mating-induced
 increases in susceptibility to infections in Drosophila melanogaster.
description: # Selection for increased post-infection survival ameliorates mating
 induced immune suppression in Drosophila melanogaster females
 [https://doi.org/10.5061/dryad.fbg79cnxn](https://doi.org/10.5061/dryad.fbg79cnxn) ## Description of the data and file structure This file ("malesEf.csv") was generated in 2019 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali. GENERAL INFORMATION 1. Title of Dataset: "Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females" 2. Author Information A. Principal Investigator Contact Information Name: Prof. N. G. Prasad Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [prasad@iisermohali.ac.in](mailto:prasad@iisermohali.ac.in) B. Associate or Co-investigator Contact Information Name: Aabeer Basu Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [aabeerkbasu@gmail.com](mailto:aabeerkbasu@gmail.com) 3\. Duration of data collection: June – July, 2019 4\. Geographic location of data collection: Mohali, Punjab, India  5\. Information about funding sources that supported the collection of the data: IISER Mohali, Govt. of India METHODOLOGICAL INFORMATION Please refer to the bioRxiv pre-print entitled " Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females" Preprint link: [https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1](https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1) DATA & FILE OVERVIEW File Name: "malesEf.csv" Note: This file contains data for post-infection survival of virgin and mated males from the E, P, and N populations when infected with Enterococcus faecalis (as described in the above cited bioRxiv pre-print). 1\. Number of variables: 6 2\. Number of cases/rows: 2870 3\. Variable List:  Block (Block identity of the host; also serves as an indicator of ancestry.) Population (Population identity/selection history of the host. “E” denotes flies from the selected populations, “P” denotes flies from the sham-infected control populations, “N” denotes flies from the uninfected control populations.) Mating_status (Denotes whether the host was mated before being infected/sham-infected; "Virgin" denotes hosts not allowed to mate, "Mated" denotes mated hosts.) Infection_status (Denotes whether the host was subjected to infection or was sham-infected. “Infected” denotes flies infected with Enterococcus faecalis, “Sham-infected” denotes flies subjected to sham-infection.) Time (Time in hours at which the event (death/censor) was recorded.) Censor (Status of the fly at the time the event was recorded. "1" denotes mortality, "0" denotes right-censored data.) This file ("femalesPe.csv") was generated in 2019 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali. GENERAL INFORMATION 1\. Title of Dataset: "Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females" 2\. Author Information A. Principal Investigator Contact Information Name: Prof. N. G. Prasad Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [prasad@iisermohali.ac.in](mailto:prasad@iisermohali.ac.in) B. Associate or Co-investigator Contact Information Name: Aabeer Basu Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [aabeerkbasu@gmail.com](mailto:aabeerkbasu@gmail.com) 3\. Duration of data collection: December, 2019 4\. Geographic location of data collection: Mohali, Punjab, India 5\. Information about funding sources that supported the collection of the data: IISER Mohali, Govt. of India. METHODOLOGICAL INFORMATION Please refer to the bioRxiv pre-print entitled " Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females ". Preprint link: [https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1](https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1) DATA & FILE OVERVIEW File Name: "femalesPe.csv" Note: This file contains data for post-infection survival of virgin and mated females from the E and P populations when infected with Pseudomonas entomophila (as described in the above cited bioRxiv pre-print). 1\. Number of variables: 6 2\. Number of cases/rows: 1923 3\. Variable List:  Block (Block identity of the host; also serves as an indicator of ancestry.) Population (Population identity/selection history of the host. “E” denotes flies from the selected populations, “P” denotes flies from the sham-infected control populations.) Mating_status (Denotes whether the host was mated before being infected/sham-infected; "Virgin" denotes hosts not allowed to mate, "Mated" denotes mated hosts.) Infection_status (Denotes whether the host was subjected to infection or was sham-infected. “Infected” denotes flies infected with Pseudomonas entomophila, “Sham-infected” denotes flies subjected to sham-infection.) Time (Time in hours at which the event (death/censor) was recorded.) Censor (Status of the fly at the time the event was recorded. "1" denotes mortality, "0" denotes right-censored data.) This file ("femalesBt.csv") was generated in 2019 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali. GENERAL INFORMATION 1\. Title of Dataset: "Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females" 2\. Author Information A. Principal Investigator Contact Information Name: Prof. N. G. Prasad Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [prasad@iisermohali.ac.in](mailto:prasad@iisermohali.ac.in) B. Associate or Co-investigator Contact Information Name: Aabeer Basu Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [aabeerkbasu@gmail.com](mailto:aabeerkbasu@gmail.com) 3\. Duration of data collection: December, 2019 4\. Geographic location of data collection: Mohali, Punjab, India  5\. Information about funding sources that supported the collection of the data: IISER Mohali, Govt. of India. METHODOLOGICAL INFORMATION Please refer to the bioRxiv pre-print entitled " Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females ". Preprint link: [https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1](https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1) DATA & FILE OVERVIEW File Name: "femalesBt.csv" Note: This file contains data for post-infection survival of virgin and mated females from the E and P populations when infected with Bacillus thuringiensis (as described in the above cited bioRxiv pre-print). 1\. Number of variables: 6 2\. Number of cases/rows: 1924 3\. Variable List:  Block (Block identity of the host; also serves as an indicator of ancestry.) Population (Population identity/selection history of the host. “E” denotes flies from the selected populations, “P” denotes flies from the sham-infected control populations.) Mating_status (Denotes whether the host was mated before being infected/sham-infected; "Virgin" denotes hosts not allowed to mate, "Mated" denotes mated hosts.) Infection_status (Denotes whether the host was subjected to infection or was sham-infected. “Infected” denotes flies infected with Bacillus thuringiensis, “Sham-infected” denotes flies subjected to sham-infection.) Time (Time in hours at which the event (death/censor) was recorded.) Censor (Status of the fly at the time the event was recorded. "1" denotes mortality, "0" denotes right-censored data.) This file ("femalesEf.csv") was generated in 2019 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali. GENERAL INFORMATION 1\. Title of Dataset: "Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females" 2\. Author Information A. Principal Investigator Contact Information Name: Prof. N. G. Prasad Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [prasad@iisermohali.ac.in](mailto:prasad@iisermohali.ac.in) B. Associate or Co-investigator Contact Information Name: Aabeer Basu Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [aabeerkbasu@gmail.com](mailto:aabeerkbasu@gmail.com) 3\. Duration of data collection: June – July, 2019 4\. Geographic location of data collection: Mohali, Punjab, India  5\. Information about funding sources that supported the collection of the data: IISER Mohali, Govt. of India. METHODOLOGICAL INFORMATION Please refer to the bioRxiv pre-print entitled " Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females ". Preprint link: [https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1](https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1) DATA & FILE OVERVIEW File Name: "femalesEf.csv" Note: This file contains data for post-infection survival of virgin and mated females from the E, P, and N populations when infected with Enterococcus faecalis (as described in the above cited bioRxiv pre-print). 1\. Number of variables: 6 2\. Number of cases/rows: 2882 3\. Variable List:  Block (Block identity of the host; also serves as an indicator of ancestry.) Population (Population identity/selection history of the host. “E” denotes flies from the selected populations, “P” denotes flies from the sham-infected control populations, “N” denotes flies from the uninfected control populations.) Mating_status (Denotes whether the host was mated before being infected/sham-infected; "Virgin" denotes hosts not allowed to mate, "Mated" denotes mated hosts.) Infection_status (Denotes whether the host was subjected to infection or was sham-infected. “Infected” denotes flies infected with Enterococcus faecalis, “Sham-infected” denotes flies subjected to sham-infection.) Time (Time in hours at which the event (death/censor) was recorded.) Censor (Status of the fly at the time the event was recorded. "1" denotes mortality, "0" denotes right-censored data.) This file ("remating_rate.csv") was generated in 2019 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali. GENERAL INFORMATION 1\. Title of Dataset: "Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females" 2\. Author Information A. Principal Investigator Contact Information Name: Prof. N. G. Prasad Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [prasad@iisermohali.ac.in](mailto:prasad@iisermohali.ac.in) B. Associate or Co-investigator Contact Information Name: Aabeer Basu Institution: Indian Institute of Science Education and Research Mohali Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India. Email: [aabeerkbasu@gmail.com](mailto:aabeerkbasu@gmail.com) 3\. Duration of data collection: June – July, 2019 4\. Geographic location of data collection: Mohali, Punjab, India  5\. Information about funding sources that supported the collection of the data: IISER Mohali, Govt. of India. METHODOLOGICAL INFORMATION Please refer to the bioRxiv pre-print entitled " Selection for increased post-infection survival ameliorates mating induced immune suppression in Drosophila melanogaster females ". Preprint link: [https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1](https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1) DATA & FILE OVERVIEW File Name: "remating_rate.csv" Note: This file contains data for remating rate of males and females from the E, P, and N populations (as described in the above cited bioRxiv pre-print). 1\. Number of variables: 4 2\. Number of cases/rows: 241 3\. Variable List:  Block (Block identity of the host; also serves as an indicator of ancestry.) Population (Population identity/selection history of the host. “E” denotes flies from the selected populations, “P” denotes flies from the sham-infected control populations, “N” denotes flies from the uninfected control populations.) Sex (Sex of the host. “F” denotes females, “M” denotes males.) Vial_ID (Vial identity, nested within population and block identities. Unit of replication.) Remating_events (The number of remating events observed in each vial over a period of 300 minutes, with sampling every 15 minutes.) **SHARING/ACCESS INFORMATION** Links to publications/pre-prints that cite or use the data: (bioRxiv)  [https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1](https://www.biorxiv.org/content/10.1101/2022.07.01.498387v1)