cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/zgsj9-hkf76
title: Ecoli_EPEC_8612

description: Bacterial nanotubes as described by the work of Ilan Rosenshine and Sigal Ben Yehuda labs at  .  the Hebrew university).
description: <strong>Tilt Series Date:</strong> 2020-02-14</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> These cells were obtained from Ritesh (Ilan Rosenshine lab) and Amit (Sigal ben Yehuda lab, . both from the Hebrew university). They were present during sample preparation.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> An overnight culture from -80C stock was made in 2ml LB and was grown at 37C in static conditions. The next day, 1 ml of cells were spun down at 4000 rpm for six minutes and . concentrated 10 times. 3 ul of cells were spotted on glow-. discharged grids located on a DMEM high-glucose agar plates (1.5% agar) for 3 hours. Then, the grids were washed twice in PBS and transferred to a starvation-medium agar plates (Starvation medium:DMEM without phenol red, vitamins and . amino acids) and incubated for 1 hour at 37C. Subsequently, the samples were washed with PBS and plunge-frozen.</p>
<strong>Sample Preparation:</strong> The samples were hold with a tweezer from the starvation plates and washed with PBS. Blotted . . with different conditions (B</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-63/rawdata/</p>EPEC_8612_143_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-63/rawdata/EPEC_8612_143_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        EPEC_8612_143_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-63/3dimage_111169/EPEC_8612_143_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.wkchbm
title: Occurrence Download

description: A dataset containing 229 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Brienomyrus longianalis, Brycinus brevis, Brycinus carmesinus, Brycinus derhami, Brycinus grandisquamis, Brycinus leuciscus, Brycinus longipinnis, Brycinus luteus, Brycinus macrolepidotus, Brycinus poptae, Brycinus rhodopleura, Bryconaethiops boulengeri, Bryconaethiops macrops, Bryconaethiops microstoma, Bryconaethiops quinquesquamae, Bryconalestes derhami, Bryconalestes longipinnis, Bryconalestes tholloni, Buccochromis heterotaenia, Buccochromis lepturus, Buccochromis nototaenia, Buccochromis rhoadesii, Buccochromis spectabilis, Butis butis, Caecobarbus geertsii, Caffrogobius agulhensis, Callochromis macrops, Callochromis melanostigma, Callochromis pleurospilus, Callopanchax monroviae, Callopanchax occidentalis, Callopanchax toddi, Campylomormyrus alces, Campylomormyrus bredoi, Campylomormyrus cassaicus, Campylomormyrus christyi, Campylomormyrus curvirostris, Campylomormyrus elephas, Campylomormyrus luapulaensis, Campylomormyrus mirus, Campylomormyrus numenius, Campylomormyrus orycteropus, Campylomormyrus phantasticus, Campylomormyrus rhynchophorus, Campylomormyrus tamandua, Campylomormyrus tshokwe, Caprichromis liemi, Caprichromis orthognathus, Caranx sexfasciatus, Carasobarbus fritschii, Carasobarbus harterti, Carasobarbus moulouyensis, Carassius auratus, Carcharhinus leucas, Cardiopharynx schoutedeni, Carlarius latiscutatus, Carlarius parkii, Champsochromis caeruleus, Champsochromis spilorhynchus, Channa maculata, Channallabes alvarezi, Channallabes apus, Channallabes longicaudatus, Channallabes ogooensis, Channallabes sanghaensis, Channallabes teugelsi, Chanos chanos, Cheilochromis euchilus, Chelaethiops bibie, Chelaethiops congicus, Chelaethiops elongatus, Chelaethiops minutus, Chelaethiops rukwaensis, Chelidonichthys kumu, Chelon auratus, Chelon labrosus, Chelon melinopterus, Planiliza melinopterus, Chelon planiceps, Chelon ramada, Chelon saliens, Chelonodontops patoca, Chetia brevicauda, Chetia brevis, Chetia flaviventris, Chetia gracilis, Chetia mola, Chetia welwitschi, Chilochromis duponti, Chiloglanis angolensis, Chiloglanis anoterus, Chiloglanis asymetricaudalis, Chiloglanis batesii, Chiloglanis benuensis, Chiloglanis bifurcus, Chiloglanis brevibarbis, Chiloglanis camarabounyi, Chiloglanis cameronensis)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((10.0615 -3.4463,10.1382 -3.5292,10.1382 2.0599,9.2753 2.0599,9.2723 2.0399,8.8555 1.2955,8.8103 -0.1351,8.354 -0.2687,8.2157 -0.7924,8.7426 -1.8029,8.6574 -2.0749,8.8035 -2.4281,9.149 -2.574,10.0615 -3.4463))"
 ]
}

 The dataset includes 229 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006357-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/51d9s-a6c71
title: lip9-3 (+ megacomplex)

description: Exposure 1s
description: <strong>Tilt Series Date:</strong> 2020-10-05</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK2, BL21 dHisdHA RQABpCDF+EFGpRSF</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 88.0 eV/Å²,  defocus: -10.0 μm,  magnification: 11000x. </p>
<strong>Microscope:</strong> IECB Talos Artica</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition</p>
<strong>Purification / Growth Conditions / Treatment:</strong> BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex.. . Selection done with Kanamycin and Streptomycin.. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane fractions. . Liposomes are then generated from the membrane fractions by processing . through Potter and sonicator.. </p>
<strong>Sample Preparation:</strong> Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-10-05-13/rawdata/</p>lip9-3-sq1-tg8.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-10-05-13/rawdata/lip9-3-sq1-tg8.mrc"
        > <i class="download icon"></i> Download </a></p>

        lip9-3-sq1-tg8_full.rec,  Reconstruction (Pixel Size 0.78 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-10-05-13/3dimage_115833/lip9-3-sq1-tg8_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/598e8-knc96
title: Helicobacter_Pylori_dFliO

description: These are H. pylori cells that don't have a complete FliP. In addition, FliO was knocked out in  .  these cells. The aim is to study the assembly of the flagellar motor and the core complex.
description: <strong>Tilt Series Date:</strong> 2019-09-21</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . flagella. We knocked out FliO protein of the flagellar motor to study the early assembly . . states of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1. 100% humidity. Cu grids. 10 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-10/rawdata/</p>Hpylori_dFliO8.mrc,  Tilt Series (Pixel Size 0.4494 nm), 2.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-10/rawdata/Hpylori_dFliO8.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_dFliO8.mrc.mdoc,  27.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-21-10/rawdata/Hpylori_dFliO8.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b5y91-50g53
title: H-pylori_dFliM_GC

description: These are fast tilt series taken by Georges Chreifi on the Krios for dFliM mutant of Helicobacter  .  pylori.
description: <strong>Tilt Series Date:</strong> 2019-10-28</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild type cells but they . don't express complete FliP protein. Therefore, they don't assemble full flagella. We knocked out FliM protein . of the flagellar motor to study the early assembly states of flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100% humidity. Cu grids. 10 nm . gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-28-117/rawdata/</p>Hpylori_FliM_119_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 3.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-28-117/rawdata/Hpylori_FliM_119_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_FliM_119_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-28-117/3dimage_107907/Hpylori_FliM_119_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/w2k3t-1q985
title: lip10-2 (+ megacomplex)

description: Exposure 1-1.3s
description: <strong>Tilt Series Date:</strong> 2020-09-29</p>
<strong>Data Taken By:</strong> William Nicolas</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> PK2, BL21 dHisdHA RQABpCDF+EFGpRSF</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Å²,  defocus: -8.0 μm,  magnification: 11000x. </p>
<strong>Microscope:</strong> IECB Talos Artica</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition</p>
<strong>Purification / Growth Conditions / Treatment:</strong> BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex.. . Selection done with Kanamycin and Streptomycin.. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane . fractions. . Liposomes are then generated from the membrane fractions by . processing through Potter and sonicator.. </p>
<strong>Sample Preparation:</strong> Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-38/rawdata/</p>lip10-2-sq3-tg7.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-38/rawdata/lip10-2-sq3-tg7.mrc"
        > <i class="download icon"></i> Download </a></p>

        lip10-2-sq3-tg7_full.rec,  Reconstruction (Pixel Size 0.78 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-38/3dimage_115805/lip10-2-sq3-tg7_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a9nv5-wvf34
title: Pseudomonas aerugionsa PA103

description: This strain was obtained from Alan Hauser's lab from Northwestern University. It contains a plasmid containing the FtsZ gene. The over-expression of FtsZ will produce minicells. Other than the plasmid, this strain is the same as the wild type.
description: <strong>Tilt Series Date:</strong> 2019-06-19</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 95.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Jensen Lab tomography pipeline (RAPTOR for alignment)</p>
<strong>Collaborators and Roles:</strong> Alan Hauser lab from Northwestern University</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The cells were grown in LB with 50 ug/mL gentamicin. Incubate the cells for 1 hour at 37 C 200 rpm at a dilution factor of  50. Then add IPTG to reach a final concentration of 1 mM. Incubate for 6 hours at 37 C at 200 rpm. Then remove most of the filamentous cells by 3000xg centrifugation for 10 min. Collect the minicells from the supernatant with another centrifugation at 15,000xg for 30 min.</p>
<strong>Sample Preparation:</strong> Mix 40 uL of cells with gold beads concentrated from 200 uL Au solution + 50 uL 5% BSA. Plunge freeze the samples with liquid ethane and propane with a blotting force of 4, a blotting time of 3 s or 4s and a draining time of 1 s.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-19-15/rawdata/</p>Pa_cell_target_7.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-19-15/rawdata/Pa_cell_target_7.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pa_cell_target_7_full.rec,  Reconstruction (Pixel Size 1.8 nm), 589.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-19-15/3dimage_104005/Pa_cell_target_7_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xy7gn-gpg41
title: Hpylori_dFliM

description: These are fast tilt series that I collected on Hpylori_dFliM (dFliP also) on the Titan Krios.
description: <strong>Tilt Series Date:</strong> 2019-11-24</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> I received these cells from Carrie Schaffer from the university of Kentucky. These are wild type cells but they don't express complete FliP . protein. Therefore, they don't assemble full flagella. We knocked out FliM protein of the flagellar motor to study the early assembly states of . flagellar motor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown for 48 hours and then spread on a new plate with a cotton swab and left to . grow for 24 hours. The grids were prepared from both batches (48 hours, and 24 hours, separately).</p>
<strong>Sample Preparation:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100% . humidity. Cu grids. 10 nm gold.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-11-24-88/rawdata/</p>Hpylori_dfliM_090_af.mrc,  Tilt Series (Pixel Size 0.449 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-11-24-88/rawdata/Hpylori_dfliM_090_af.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_dfliM_090_af_full.rec,  Reconstruction (Pixel Size 1.796 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-11-24-88/3dimage_110043/Hpylori_dfliM_090_af_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/7sp72-7tm98
title: Pseudomonas_aeruginosa_DfliP

description: This sample was imaged to check for the presence of the tentative flagellar inner-membrane- .  embedded structures in FliP knockout
description: <strong>Tilt Series Date:</strong> 2019-05-25</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> None</p>
<strong>Collaborators and Roles:</strong> I obtained these cells from Dianne Newman lab from the transposon library (mutant 10239 from . the full library excel file). Shannon Park helped me to get the cells.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were first grown on LB plate with 100 ug/ml gentamicin over night (from -80 C stock). . Next dat, one colony was inoculated in 2 ml of LB (with 100 ug/ml gentamicin) and incubated . for 16 hours at 37 C with 175 rpm. After cells were mixed with gold beads (coated with BSA), . 4 ul were added to the grids, blotted and plunge frozen.</p>
<strong>Sample Preparation:</strong> Different plunge-freezing parameters: 1) blot force 6, blot time 3s, drain time 1s. 2) blot force 3, . blot time 2s, drain time 1s. 3) blot force -5, blot time 4s, drain time 1s. 4) blot force -3, blot time . 2s, drain time 1s. All conditions were at room temperature with 100 % humidity.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-1/rawdata/</p>Squ10_Montage.mrc,  Tilt Series (Pixel Size 0.449 nm), 264.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-1/rawdata/Squ10_Montage.mrc"
        > <i class="download icon"></i> Download </a></p>

        Squ10_Montage.mrc.mdoc,  16.6 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-05-25-1/rawdata/Squ10_Montage.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>