cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7591502.v1
title: Biotechnological Properties of Sponges from Northeast Brazil: Cliona varians as a Biocatalyst for Enantioselective Reduction of Carbonyl Compounds

description: To research the potential ability of whole marine sponges to act as biocatalysts, this paper describes for the first time the employment of whole Cliona varians sponge in the stereoselective reduction of prochiral a-keto esters and isatin to the corresponding chiral alcohols. The addition of D-fructose, D-glucose or sucrose remarkably increased the conversion ratios and stereoselectivities by this marine sponge. Furthermore, in the presence of D-glucose and D-maltose, the reduction of isatin by C. varians afforded the corresponding 3-hydroxyindolin-2-one with high conversions (85-90%) and good enantioselectivities (60-74%). These results showed that the marine sponge presents great potential to be used as biocatalyst for stereoselective reduction of carbonyl compounds.
DOI: 10.6084/m9.figshare.7591496.v1
title: Density Determination of immiscible liquids by the Stevin's principle

description: Abstract This work presents a didactic sequence followed by a low cost, easy to set up and easy to operate, experimental apparatus applied to the study of hydrostatics and, particularly, of a composition of immiscible liquids like water and oil. The Stevin Principle is used to analyze density of such liquids, having density of water (ρ = 1000 kg/m3) as reference. The teacher has a fundamental role in this proposal, since he is the mediator of the whole process, allowing the construction of connections between the existing concepts in student's cognitive structure with the new themes. The physical concepts were built through an expositive class, discussing on the physical concepts, history and everyday applications, as a form to better organize the previous knowledge of the students. Finally, an integrative reconciliation was made during the experimentation, intended to better provide meaningful learning. With the results, besides the observation that, in any given recipient, lower density liquids positionate itself above higher density ones, the students also determine the densities of such liquids, obtaining values similar to those found in literature. For example, the density of soy oil was determined as 0,87 g/cm3 using the proposed experiment, against an accepted value of 0,891 g/cm3 , a difference of about 2%.
DOI: 10.7284/115739
title: SSV (Sound Speed Velocimeter) data collected during research cruise FK140307 using a Valeport MiniSVS instrument system onboard the platform RV Falkor

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7591499.v1
title: Comparison of choroidal thickness in patients with active and stable thyroid eye disease

description: ABSTRACT Purpose: To compare the choroidal thickness in active and stable phases of thyroid eye disease. Methods: Forty-seven eyes of 47 patients with thyroid eye disease were prospectively studied. Patients were evaluated on the basis of their clinical activity scores, with scores 33 defined as active disease. Subfoveal, temporal macular, nasal macular, temporal peripapillary, and nasal peripapillary choroidal thickness measurements were performed with Cirrus enhanced depth imaging spectral-domain optical coherence tomography, and the results in the two groups were compared. Results: Twenty-four patients were int he active group, whereas 23 patients were in the stable group. Choroidal thickness was significantly higher in the subfoveal and temporal macular regions in the active group. Although the nasal macular and peripapillary values were also higher in the active group, the difference was insignificant. Conclusions: Subfoveal choroidal thickness was significantly higher in patients with thyroid eye disease in the active phase than in those with stable phase disease.
DOI: 10.6084/m9.figshare.7591487
title: Evaluation of nonconformities related to sanitary regulations applied to clinical laboratories in public hospital in the city of Rio de Janeiro-RJ from November 2016 to November 2017

description: ABSTRACT The study presents the main nonconformities related to the sanitary regulations found in clinical laboratory analysis (CLA) attached to a public hospital in the city of Rio de Janeiro (RJ) from November 2016 to November 2017. The evaluation of the nonconformities related to sanitary conditions is fundamental to ensure the goals in improving quality control, increasing the reliability of the results generated and reducing health risks. Through the evaluation of 20 federal and municipal public health laboratories, it was possible to analyze the main sanitary nonconformities in the different laboratories phases (pre-analytical, analytical and post-analytical), evaluating, through Fisher's exact test, the frequency and trend distribution of reported nonconformities. One hundred percent (100%) of the clinical analysis laboratories presented at least, one nonconformity. Among those with the highest frequencies of nonconformity related to sanitary conditions are those related to the lack of standard operating procedures (SOPs), presenting 45.5% in clinical analysis laboratories of federal hospitals and 66.7% in clinical analysis laboratories of municipal hospitals. Moreover flaws in the cleaning, disinfection and sterilization processes, in equipments and in the presence of the technical manager (TM) throughout the working hours. Sanitary surveillance actions seek to provide health services to the population that comply with established quality standards, even though the identification of nonconformities subsidizes the adoption of corrective actions by the health establishment.
DOI: 10.7284/121422
title: TSG (Thermosalinograph) data collected during research cruise FK140307 using a Sea-Bird SBE-45 instrument system onboard the platform RV Falkor

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7591481
title: Soil carbon and physical-mechanical properties after successive applications of swine and poultry organic waste

description: ABSTRACT In agricultural crops, the use of swine and poultry waste as organic fertilizers results in gains in the productivity and reduction of production costs, but it may also change the physical properties and mechanical behavior of the soil, either increasing or reducing its quality. This study aimed to investigate the influence of applying increasing doses of liquid swine manure (160 m³ ha-1 year-1, 320 m³ ha-1 year-1 and 480 m³ ha-1 year-1) and poultry litter (6 Mg ha-1 year-1, 12 Mg ha-1 year-1 and 18 Mg ha-1 year-1), along four years, on the physical-mechanical properties and organic carbon content of a very clayey Rhodic Eutrudox. The application of liquid swine manure and poultry litter increased the surface soil organic carbon contents. The soil density decreased, whereas the total porosity and macroporosity increased, with the addition of liquid swine manure and poultry litter. There were no significant changes in the soil susceptibility to compaction.
DOI: 10.6084/m9.figshare.7591487.v1
title: Evaluation of nonconformities related to sanitary regulations applied to clinical laboratories in public hospital in the city of Rio de Janeiro-RJ from November 2016 to November 2017

description: ABSTRACT The study presents the main nonconformities related to the sanitary regulations found in clinical laboratory analysis (CLA) attached to a public hospital in the city of Rio de Janeiro (RJ) from November 2016 to November 2017. The evaluation of the nonconformities related to sanitary conditions is fundamental to ensure the goals in improving quality control, increasing the reliability of the results generated and reducing health risks. Through the evaluation of 20 federal and municipal public health laboratories, it was possible to analyze the main sanitary nonconformities in the different laboratories phases (pre-analytical, analytical and post-analytical), evaluating, through Fisher's exact test, the frequency and trend distribution of reported nonconformities. One hundred percent (100%) of the clinical analysis laboratories presented at least, one nonconformity. Among those with the highest frequencies of nonconformity related to sanitary conditions are those related to the lack of standard operating procedures (SOPs), presenting 45.5% in clinical analysis laboratories of federal hospitals and 66.7% in clinical analysis laboratories of municipal hospitals. Moreover flaws in the cleaning, disinfection and sterilization processes, in equipments and in the presence of the technical manager (TM) throughout the working hours. Sanitary surveillance actions seek to provide health services to the population that comply with established quality standards, even though the identification of nonconformities subsidizes the adoption of corrective actions by the health establishment.
DOI: 10.6084/m9.figshare.7591475
title: Evaluation of platelet activity by multiple electrode impedance aggregometry in acute coronary syndromes: pilot study in a Brazilian tertiary-care public hospital

description: There is no definite recommendation for testing platelet aggregation (PA) in acute coronary syndromes (ACS) due to inconclusive evidence on the usefulness of platelet function tests to guide therapy and improve clinical outcomes. The evaluation of PA with multiple electrode impedance platelet aggregometry (MEA) may be useful to manage antiplatelet therapy and possibly influence patient outcome. The primary aim of this study was to measure PA with MEA in Brazilian patients with ACS and evaluate the association between PA and adverse clinical outcomes. Forty-seven consecutive patients admitted with ACS to a Brazilian tertiary-care public hospital were studied and PA was evaluated using MEA. Patients were followed for six months for the occurrence of all-cause death, acute myocardial infarction, or stroke. Suboptimal inhibition of PA was found in 7 patients (14.9%); 5 (10.6%) in response to ASA (acetylsalicylic acid), 2 (5.0%) to clopidogrel, and none to ticagrelor. Inadequate PA inhibition in response to ASA was significantly associated with the composite end point, but there was no significant association for insufficient PA inhibition in response to clopidogrel. This study suggested that the evaluation of PA in ACS using MEA may identify non-responders to ASA. Larger studies are necessary to define, in a public health scenario, the value of MEA in the management of ACS.
DOI: 10.6084/m9.figshare.7591478
title: Synthesis of Spiro-Pyrrolidinyloxindoles by Oxidative Rearrangement of N-Acyltetrahydro-β-carbolines Using an Oxone/Aqueous Acetone Mixture

description: Spiro-pyrrolidinyl-2-oxindoles were prepared by the oxidative rearrangement of Na-acetyl-1,2,3,4-tetrahydro-β-carbolines (THBC) using dimethyldioxirane generated in situ. The Na-acetyl THBC substrates were obtained by Pictet-Spengler and acyl-Pictet-Spengler reactions of L-tryptophan methyl ester, followed by Na-acetylation. The stereoselectivity of the oxidative rearrangement was evaluated and 2D nuclear magnetic resonance (NMR) was used to determine the stereochemistry of the oxindole products relative to L-tryptophan. Density functional theory calculations were consistent with a face selective, substrate controlled, epoxidation of the indolic double bond. The calculations indicated that the resulting epoxide would readily rearrange at room temperature via a concerted ring opening/ring contraction process to give the 3-substituted-2-oxindole.
DOI: 10.6084/m9.figshare.7591478.v1
title: Synthesis of Spiro-Pyrrolidinyloxindoles by Oxidative Rearrangement of N-Acyltetrahydro-β-carbolines Using an Oxone/Aqueous Acetone Mixture

description: Spiro-pyrrolidinyl-2-oxindoles were prepared by the oxidative rearrangement of Na-acetyl-1,2,3,4-tetrahydro-β-carbolines (THBC) using dimethyldioxirane generated in situ. The Na-acetyl THBC substrates were obtained by Pictet-Spengler and acyl-Pictet-Spengler reactions of L-tryptophan methyl ester, followed by Na-acetylation. The stereoselectivity of the oxidative rearrangement was evaluated and 2D nuclear magnetic resonance (NMR) was used to determine the stereochemistry of the oxindole products relative to L-tryptophan. Density functional theory calculations were consistent with a face selective, substrate controlled, epoxidation of the indolic double bond. The calculations indicated that the resulting epoxide would readily rearrange at room temperature via a concerted ring opening/ring contraction process to give the 3-substituted-2-oxindole.
DOI: 10.6084/m9.figshare.7591481.v1
title: Soil carbon and physical-mechanical properties after successive applications of swine and poultry organic waste

description: ABSTRACT In agricultural crops, the use of swine and poultry waste as organic fertilizers results in gains in the productivity and reduction of production costs, but it may also change the physical properties and mechanical behavior of the soil, either increasing or reducing its quality. This study aimed to investigate the influence of applying increasing doses of liquid swine manure (160 m³ ha-1 year-1, 320 m³ ha-1 year-1 and 480 m³ ha-1 year-1) and poultry litter (6 Mg ha-1 year-1, 12 Mg ha-1 year-1 and 18 Mg ha-1 year-1), along four years, on the physical-mechanical properties and organic carbon content of a very clayey Rhodic Eutrudox. The application of liquid swine manure and poultry litter increased the surface soil organic carbon contents. The soil density decreased, whereas the total porosity and macroporosity increased, with the addition of liquid swine manure and poultry litter. There were no significant changes in the soil susceptibility to compaction.
DOI: 10.6084/m9.figshare.7591472
title: Sample Preparation Applied to Analysis of Proteins in Cowpea Seeds (Vigna unguiculata (L.) Walp.)

description: In this study, 20 different protocols for protein extraction of cowpea seeds in natura were evaluated taking into account four parameters: cell disruption method, time, temperature and solvent. Factorial design experiments were executed to determine the effects on the total protein content of extracts. The total protein content was determined and the results were in the range of 8.75 ± 1.3 and 86.6 ± 6.9 mg g-1. The richest protein extracts were obtained using Tris-HCl buffer at 50 ºC for 20 min for each assessed cell disruption method. However, the results using water were similar to buffer according to t-test at the 95% confidence level. Six protocols demonstrated higher extraction efficiency. Comparative proteomic study using size exclusion chromatography coupled to diode array detector (SEC-DAD) revealed that the one protocol extracted simultaneously high and low molecular mass biomolecules. In fact, the effect of microwave radiation promoted an increased solubility of biomolecules in water. The analysis of chromatographic profiles for principal component analysis (PCA) highlights the greater efficiency of extraction with the application of this protocol. Therefore, our study showed that the optimum condition for extraction of cowpea seed protein consists of water assisted by microwave radiation at 50 ºC for 20 min.
DOI: 10.6084/m9.figshare.7591472.v1
title: Sample Preparation Applied to Analysis of Proteins in Cowpea Seeds (Vigna unguiculata (L.) Walp.)

description: In this study, 20 different protocols for protein extraction of cowpea seeds in natura were evaluated taking into account four parameters: cell disruption method, time, temperature and solvent. Factorial design experiments were executed to determine the effects on the total protein content of extracts. The total protein content was determined and the results were in the range of 8.75 ± 1.3 and 86.6 ± 6.9 mg g-1. The richest protein extracts were obtained using Tris-HCl buffer at 50 ºC for 20 min for each assessed cell disruption method. However, the results using water were similar to buffer according to t-test at the 95% confidence level. Six protocols demonstrated higher extraction efficiency. Comparative proteomic study using size exclusion chromatography coupled to diode array detector (SEC-DAD) revealed that the one protocol extracted simultaneously high and low molecular mass biomolecules. In fact, the effect of microwave radiation promoted an increased solubility of biomolecules in water. The analysis of chromatographic profiles for principal component analysis (PCA) highlights the greater efficiency of extraction with the application of this protocol. Therefore, our study showed that the optimum condition for extraction of cowpea seed protein consists of water assisted by microwave radiation at 50 ºC for 20 min.
DOI: 10.7284/115731
title: Expendable Probe data collected during research cruise FK140418 using a Turo Quoll instrument system onboard the platform RV Falkor

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7591466
title: Social Anxiety Disorder in the Context of Problem Based-Learning

description: ABSTRACT Objective To investigate the symptoms of social anxiety disorder (SAD) among medical students of the Christus University Center (Unichristus), an institution that adopts the problem-based learning (PBL) method. Methods A cross-sectional study with 431 students in their first to fourth years of Medical School, through the application of the Liebowitz Social anxiety scale (LSAS-SR), Beck’s anxiety Inventory, and a questionnaire with questions concerning sociodemographic issues, family history of psychiatric disorders, psychiatric appointments, level of anxiety when expressing themselves in tutorials, and the perception of how their anxiety varied throughout the college semesters. Results Using the LSAS-SR as a screening tool for cases of SAD, there were indications of the disorder in 59.2% (255) of the students. In relation to the level of anxiety, the Beck’s anxiety inventory (BAI) showed that 59.3% (258) of the students presented minimal anxiety, 26.6% (115) presented light anxiety, 9.7% (42) presented moderate anxiety, and 4.4% (19) presented severe anxiety. A significant correlation was observed between the scores obtained in the BAI and LSAS-SR scales: students with symptoms suggesting social anxiety showed a greater degree of anxiety. Also, students with symptoms of social anxiety reported higher levels of anxiety when expressing themselves during tutorials. Conclusions The high prevalence of SAD pointed out in this research justifies stimulating the adoption of psycho-educational measures and pedagogical strategies that will help students with the manifestations of social anxiety, reduce these symptoms, and promote the teaching-learning process.
DOI: 10.6084/m9.figshare.7591475.v1
title: Evaluation of platelet activity by multiple electrode impedance aggregometry in acute coronary syndromes: pilot study in a Brazilian tertiary-care public hospital

description: There is no definite recommendation for testing platelet aggregation (PA) in acute coronary syndromes (ACS) due to inconclusive evidence on the usefulness of platelet function tests to guide therapy and improve clinical outcomes. The evaluation of PA with multiple electrode impedance platelet aggregometry (MEA) may be useful to manage antiplatelet therapy and possibly influence patient outcome. The primary aim of this study was to measure PA with MEA in Brazilian patients with ACS and evaluate the association between PA and adverse clinical outcomes. Forty-seven consecutive patients admitted with ACS to a Brazilian tertiary-care public hospital were studied and PA was evaluated using MEA. Patients were followed for six months for the occurrence of all-cause death, acute myocardial infarction, or stroke. Suboptimal inhibition of PA was found in 7 patients (14.9%); 5 (10.6%) in response to ASA (acetylsalicylic acid), 2 (5.0%) to clopidogrel, and none to ticagrelor. Inadequate PA inhibition in response to ASA was significantly associated with the composite end point, but there was no significant association for insufficient PA inhibition in response to clopidogrel. This study suggested that the evaluation of PA in ACS using MEA may identify non-responders to ASA. Larger studies are necessary to define, in a public health scenario, the value of MEA in the management of ACS.
DOI: 10.6084/m9.figshare.7591466.v1
title: Social Anxiety Disorder in the Context of Problem Based-Learning

description: ABSTRACT Objective To investigate the symptoms of social anxiety disorder (SAD) among medical students of the Christus University Center (Unichristus), an institution that adopts the problem-based learning (PBL) method. Methods A cross-sectional study with 431 students in their first to fourth years of Medical School, through the application of the Liebowitz Social anxiety scale (LSAS-SR), Beck’s anxiety Inventory, and a questionnaire with questions concerning sociodemographic issues, family history of psychiatric disorders, psychiatric appointments, level of anxiety when expressing themselves in tutorials, and the perception of how their anxiety varied throughout the college semesters. Results Using the LSAS-SR as a screening tool for cases of SAD, there were indications of the disorder in 59.2% (255) of the students. In relation to the level of anxiety, the Beck’s anxiety inventory (BAI) showed that 59.3% (258) of the students presented minimal anxiety, 26.6% (115) presented light anxiety, 9.7% (42) presented moderate anxiety, and 4.4% (19) presented severe anxiety. A significant correlation was observed between the scores obtained in the BAI and LSAS-SR scales: students with symptoms suggesting social anxiety showed a greater degree of anxiety. Also, students with symptoms of social anxiety reported higher levels of anxiety when expressing themselves during tutorials. Conclusions The high prevalence of SAD pointed out in this research justifies stimulating the adoption of psycho-educational measures and pedagogical strategies that will help students with the manifestations of social anxiety, reduce these symptoms, and promote the teaching-learning process.
DOI: 10.7284/115599
title: Fluorometer data collected during research cruise FK140418 using a WET Labs ECO-FLNTU instrument system onboard the platform RV Falkor

description: Original (unprocessed) data collected during a Field_expedition