cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/0ez08-2dt08
title: Hippocampal Neurons (cultured)

description: Raw data files of Hippocampal Neurons (cultured)
description: <strong>Tilt Series Date:</strong> 2015-02-15</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 12 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-15-20/rawdata/</p>021515_hipp_neuron0017.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-15-20/rawdata/021515_hipp_neuron0017.mrc"
        > <i class="download icon"></i> Download </a></p>

        021515_hipp_neuron0017_full.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-15-20/3dimage_51954/021515_hipp_neuron0017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/88w1c-0ej37
title: Hippocampal Neurons (cultured)

description: Raw data files of Hippocampal Neurons (cultured)
description: <strong>Tilt Series Date:</strong> 2015-02-16</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Rat Hippocampal Neuron (cultured)</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Plunge frozen at DIV 12 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.</p>
<strong>Sample Preparation:</strong> Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-16-8/rawdata/</p>021615_hipp_neuron_hole90002.mrc,  Tilt Series (Pixel Size 0.41 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-16-8/rawdata/021615_hipp_neuron_hole90002.mrc"
        > <i class="download icon"></i> Download </a></p>

        021615_hipp_neuron_hole90002_full.rec,  Reconstruction (Pixel Size 1.64 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-02-16-8/3dimage_51994/021615_hipp_neuron_hole90002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/prkx5-rf063
title: Myxococcus xanthus SA6892

description: Strain SA6892 (minor pilin cluster 2+3 deletion, Δmxan1021-1017; Δmxan1369-1365).Grid box YWC222
description: <strong>Tilt Series Date:</strong> 2016-08-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-64/rawdata/</p>20160822_YWC222_10065.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-64/rawdata/20160822_YWC222_10065.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160822_YWC222_10065_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-22-64/3dimage_78423/20160822_YWC222_10065_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8mas5-3kx36
title: Planktonic cell of Proteus

description: Planktonic cell, PG layer, Proteus mirabillis
description: <strong>Tilt Series Date:</strong> 2016-06-22</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Proteus mirabilis</p>
<strong>Strain:</strong> HI4320</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo</p>
<strong>Collaborators and Roles:</strong> Douglas B. Weibel lab, Wisconsin-Madison university</p>
<strong>Purification / Growth Conditions / Treatment:</strong> OD=6, 4 ul loading. blotting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh</p>
<strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 195/A2. defocus: -10um. magnification: 22500.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-22-38/rawdata/</p>newsquare10012.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-22-38/rawdata/newsquare10012.mrc"
        > <i class="download icon"></i> Download </a></p>

        newsquare10012_full.rec,  Reconstruction (Pixel Size 1.92 nm), 311.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2016-06-22-38/3dimage_75975/newsquare10012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.gz4exg
title: Occurrence Download

description: A dataset containing 925 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Spiraea japonica",
 "Country is Netherlands",
 "HasCoordinate is true"
 ]
}

 The dataset includes 925 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0002694-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/zkfh6-b9194
title: T5_1550Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1550 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-03-10</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. The humidifier was not working. I wouldn't really trust the conditions. See pages 104-105</p>
<strong>Sample Preparation:</strong> The sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-5/rawdata/</p>sq7_T5_1693cysAulabelled0005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-5/rawdata/sq7_T5_1693cysAulabelled0005.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq7_T5_1693cysAulabelled0005.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-5/3dimage_83108/sq7_T5_1693cysAulabelled0005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/16h29-krf94
title: Caulobacter crescentus

description: Raw data files of Caulobacter crescentus
description: <strong>Tilt Series Date:</strong> 2015-10-01</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> AZ145, ΔldpF Pvan-dipM gentR</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Zielinska, A., Billini, M., Moll, A., Kremer, K., Briegel, A., Izquierdo Martinez, A., Jensen, G. J. and Thanbichler, M. (2017). Lytm factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106, 419-438.grown with 0.5 mM Vanillat</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-53/3dimage_69836/</p>CaulobacterAZ145firstsetB0003_part121_2.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-53/3dimage_69836/CaulobacterAZ145firstsetB0003_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        CaulobacterAZ145firstsetB0003.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-10-01-53/rawdata/CaulobacterAZ145firstsetB0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/3g9y8-ver44
title: T5_504Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 504 has been mutated to Cys residue. Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-02-17</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.See pages 104-105</p>
<strong>Sample Preparation:</strong> Sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-17-50/rawdata/</p>sq50009.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-17-50/rawdata/sq50009.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq50009.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-02-17-50/3dimage_82536/sq50009.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9pr1f-c0410
title: Azospirillum delta Operon1 and 4

description: Raw data files of Azospirillum delta Operon1 and 4
description: <strong>Tilt Series Date:</strong> 2015-04-15</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Azospirillum brasilense</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: 8.0 μm,  magnification: 34000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Gladys Alexandre, University of Tennessee</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow overnight in 5 mL MMAB+N + 200 μg/mL Ampicillin at RT (on bench) without shaking- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N + 200 μg/mL Ampicillin- Leave at RT (on bench) without shaking overnight</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-27/rawdata/</p>AzoDEltoOp1Op4April0017.mrc,  Tilt Series (Pixel Size 0.32 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-27/rawdata/AzoDEltoOp1Op4April0017.mrc"
        > <i class="download icon"></i> Download </a></p>

        AzoDEltoOp1Op4April0017_full.rec,  Reconstruction (Pixel Size 1.28 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-04-15-27/3dimage_54114/AzoDEltoOp1Op4April0017_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pbv9c-kds56
title: Myxococcus xanthus delta-pilT

description: Grid box YWC129
description: <strong>Tilt Series Date:</strong> 2015-04-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-27/rawdata/</p>20150428_YWC129_10028.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-27/rawdata/20150428_YWC129_10028.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150428_YWC129_10028_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-27/3dimage_55029/20150428_YWC129_10028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/fzhje-gh624
title: T5_1550Cys_Aumalemaide_5nm

description: This is a sample in which N terminus residue 1550 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
description: <strong>Tilt Series Date:</strong> 2017-03-10</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> EC93 stick, UPEC536</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Zach Ruhe and Chris Hayes</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Freezing in vitrobot. The humidifier was not working. I wouldn't really trust the conditions. See pages 104-105</p>
<strong>Sample Preparation:</strong> The sample prepared by Taylor Steven</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-10/rawdata/</p>sq1_T5_1693cysAulabelled0003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-10/rawdata/sq1_T5_1693cysAulabelled0003.mrc"
        > <i class="download icon"></i> Download </a></p>

        sq1_T5_1693cysAulabelled0003.rec,  Reconstruction (Pixel Size 1.56 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-03-10-10/3dimage_83118/sq1_T5_1693cysAulabelled0003.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gr7q5-maz63
title: Myxococcus xanthus delta-pilT

description: Grid box YWC129
description: <strong>Tilt Series Date:</strong> 2015-04-28</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-8/rawdata/</p>20150428_YWC129_10009.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-8/rawdata/20150428_YWC129_10009.mrc"
        > <i class="download icon"></i> Download </a></p>

        20150428_YWC129_10009_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-04-28-8/3dimage_54993/20150428_YWC129_10009_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r3406-79m47
title: H. pylori 26695 WT / AGS

description: Grid box YWC243
description: <strong>Tilt Series Date:</strong> 2017-02-27</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain and sample prepared by Carrie Shaffer</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 10 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix.</p>
  ycw2017-02-27-55_slicer17834.jpg: cag T4SS wi-fi particle? ycw2017-02-27-55_slicer17835.jpg: cag T4SS wi-fi particle?
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2017-02-27-55/rawdata/</p>20170227_YWC243_20031.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
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        20170227_YWC243_20031_part61_6.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
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