DOI: 10.5061/dryad.2fr3k
title: Data from: Reliable effective number of breeders/adult census size ratios in seasonal-breeding species: opportunity for integrative demographic inferences based on capture-mark-recapture data and multilocus genotypes

description: The ratio of the effective number of breeders (Nb) to the adult census
 size (Na), Nb/ Na, approximates the departure from the standard capacity
 of a population to maintain genetic diversity in one reproductive season.
 This information is relevant for assessing population status,
 understanding evolutionary processes operating at local scales and
 unraveling how life-history traits affect these processes. However, our
 knowledge on Nb/Na ratios in nature is limited because estimation of both
 parameters is challenging. The sibship frequency (SF) method is adequate
 for reliable Nb estimation because it is based on sibship and parentage
 reconstruction from genetic marker data, thereby providing demographic
 inferences that can be compared with field-based information. In addition,
 capture-mark-recapture (CMR) robust design methods are well suited for Na
 estimation in seasonal-breeding species. We used tadpole genotypes of
 three pond-breeding amphibian species (Epidalea calamita, Hyla molleri and
 Pelophylax perezi, n = 73-96 single-cohort tadpoles / species genotyped at
 15-17 microsatellite loci) and candidate parental genotypes (n = 94-300
 adults / species) to estimate Nb by the SF method. To assess the
 reliability of Nb estimates, we compared sibship and parentage inferences
 with field-based information and checked for the convergence of results in
 replicated subsampled analyses. Finally, we used CMR data from a 6-year
 monitoring program to estimate annual Na in the three species and
 calculate the Nb/Na ratio. Reliable ratios were obtained for E. calamita
 (Nb/Na = 0.18-0.28) and P. perezi (0.5), but in H. molleri Na could not be
 estimated and genetic information proved insufficient for reliable Nb
 estimation. Integrative demographic studies taking full advantage of SF
 and CMR methods can provide accurate estimates of the Nb/Na ratio in
 seasonal-breeding species. Importantly, the SF method provides results
 that can be readily evaluated for reliability. This represents a good
 opportunity for obtaining robust demographic inferences with wide
 applications for evolutionary and conservation research.
description: Microsatellite genotypes
 of Epidalea calamitaDNA microsatellite
 genotypes of tadpoles and adult males and females of Epidalea calamita.
 The sizes of the alleles (in base pairs) of each individual at 16 loci are
 written in a single row. Missing data are coded as
 '0'.Ecalamita_genotypes_colony.csvMicrosatellite genotypes of Hyla molleriDNA microsatellite genotypes of tadpoles and adult males and females of Hyla molleri. The sizes of the alleles (in base pairs) of each individual at 18 loci are written in a single row. Missing data are coded as '0'.Hmolleri_genotypes_colony.csvMicrosatellite genotypes of Pelophylax pereziDNA microsatellite genotypes of tadpoles and adult males and females of Pelophylax perezi. The sizes of the alleles (in base pairs) of each individual at 15 loci are written in a single row. Missing data are coded as '0'.Pperezi_genotypes_colony.csvCapture-mark-recapture histories of Epidalea calamita, Hyla molleri and Pelophylax pereziThree .inp files are included, each one comprising the capture-mark-recapture (CMR) histories of each species used for MARK analyses in the manuscript. An additional Excel file shows information about the dates of CMR sessions, the sex of each individual (males coded as 10 and females coded as 01 in the inp files) and the total captures for each individual and in each CMR session.CMR_histories.zip