cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.20296257.v1
title: Additional file 2 of An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome

description: Additional file 2. Table S1. Proteins containing ubiquitinated sites identified by four previous ubiquitome analyses.
DOI: 10.17632/4w3ytxrzxt.1
title: Comprehensive experimental analysis of the vibration serviceability due to dynamic interaction between footbridge with accurate multi-axial dynamic sensitivity and human-induced gait loads

description: Recent advances in structural engineering have facilitated the construction of large-scale structures by using lightweight and high-strength materials. However, despite innovative design techniques, urban footbridges are susceptible to excessive vibrations induced by human activity. Although vandalism human loads have the greatest dynamic impact on the response of footbridges, owing to their intensity and wide frequency range of affectation, human-induced gait loads have the highest recurrence, randomness, and capacity to limit the serviceability of this type of structure. Traditionally, the effects of the human-structure interaction (HSI) on large-scale footbridges have been evaluated for the structural direction that is most prone to present a dynamic sensitivity or dynamic coupling with pedestrian-induced loads. Nevertheless, these uni-axial analyses on urban large-scale footbridges do not consider the multi-directional effects of the HSI or are limited by the dynamics of the structure itself, which generates the need for experimental information and evaluation of these structures. In this study, an extensive and compressive investigation on the experimental assessment of HSI effects in a functional urban footbridge with accurate multiaxial dynamic sensitivity in relation to the typical frequency range of human-induced gait loads was performed. Initially, the reference structure was described and identified using an operational modal analysis (OMA) with structural outputs based on ambient vibrations. Subsequently, a comprehensive and detailed experimental human gait campaign was conducted for various load densities and gait conditions. The results demonstrate that multi-axial HSI effects were concentrated on the lateral fundamental frequency of the reference footbridge, there was an increase of the damping ratios in relation to the load density level of the structure with the greatest effect when the human gait is synchronous, changes in the behavior of human-induced gait loads with HSI effects compared to the rigid surface, the reference structure has serviceability limitations even for load densities considered as moderate, and has the potential to be used as an international benchmark.
DOI: 10.17632/5ncd732tny.1
title: Teaching creativity at initial teacher training in any subject-based class

description: Creativity is something that must be developed during school age and, therefore, must be incorporated into the classroom. In this sense, teachers play a fundamental role in building a successful creative environment. However, not enough attention is paid to creativity during teacher training. To help future teachers learn how to include creative thinking in their classes, we conducted a two-stage, qualitative study of a model based on analogical transfer. In the first stage, the pre-service teachers learn the theory behind the methodology, before practicing and discussing it in class. This stage then culminates in the pre-service teachers planning a class based on analogical transfer. During the second stage, the pre-service teachers then teach this class during their practicum. The results show that only teachers who completed both of these stages were able to fully adopt the methodology. This study contributes to the literature by providing a teaching model that incorporates creativity and which can be applied to any subject-based class during initial teacher training. In this sense, our study was applied to physics and mathematics, two subjects which often fail to address the development of creative thinking.
DOI: 10.17632/5ncd732tny.2
title: Teaching creativity at initial teacher training in any subject-based class

description: Creativity is something that must be developed during school age and, therefore, must be incorporated into the classroom. In this sense, teachers play a fundamental role in building a successful creative environment. However, not enough attention is paid to creativity during teacher training. To help future teachers learn how to include creative thinking in their classes, we conducted a two-stage, qualitative study of a model based on analogical transfer. In the first stage, the pre-service teachers learn the theory behind the methodology, before practicing and discussing it in class. This stage then culminates in the pre-service teachers planning a class based on analogical transfer. During the second stage, the pre-service teachers then teach this class during their practicum. The results show that only teachers who completed both of these stages were able to fully adopt the methodology. This study contributes to the literature by providing a teaching model that incorporates creativity and which can be applied to any subject-based class during initial teacher training. In this sense, our study was applied to physics and mathematics, two subjects which often fail to address the development of creative thinking.
DOI: 10.6084/m9.figshare.20296269.v1
title: Additional file 5 of An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome

description: Additional file 5. Table S4. Expression analysis of all E3 ligase encoding genes.
DOI: 10.17632/39g9fgxg56.1
title: DEMOSPA0521

description: This database (named DEMOSPA0521) is composed of fifteen datasets: a group of (monthly and daily moving averages) datasets derived from population stocks and a collection of (daily, monthly and quarterly) datasets obtained from population, death, migration and birth statistics
DOI: 10.17632/pwktz88jzg.1
title: COMPARATIVE ASSESSMENT OF THE PREDICTIVE ABILITY OF ORGAN DYSFUNCTION SCALES pSOFA, PELOD 2 AND PHOENIX SCORE SEPSIS IN PEDIATRIC SEPSIS

description: Introduction. To date, there remains an urgent need to identify clinical data that can serve as valid criteria for diagnosing sepsis in children, applicable both in global settings and in situations reflecting different clinical situations. In 2024, SCCM experts presented the Phoenix Score Sepsis scale for this purpose. 
The aim of the study is a comparative assessment of the discriminatory ability of the pSOFA, PELOD 2 and Phoenix Score Sepsis scales for sepsis in children in the healthcare environment of the Russian Federation.
DOI: 10.6084/m9.figshare.20296260.v1
title: Additional file 3 of An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome

description: Additional file 3. Table S2. Ubiquitinated substrates and their corresponding E3s.
DOI: 10.17632/ddnkcgm7h4.1
title: Original data set for "Functional analysis of a first hindlimb positioning enhancer via Gdf11 expression"

description: This is an original data set for "Functional analysis of a first hindlimb positioning enhancer via Gdf11 expression" by Seiji Saito, Utsugi Kanazawa, Atsuo Iida, Tatsuya Takemoto, and Takayuki Suzuki. This is one excel file.
DOI: 10.6084/m9.figshare.12964577.v2
title: LncRNA KCNQ1OT1 inhibits the radiosensitivity and promotes the tumorigenesis of hepatocellular carcinoma via the miR-146a-5p/ACER3 axis

description: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death, and radiotherapy is currently one of the main treatments. Long non-coding RNAs (lncRNAs) are associated with the radiosensitivity and tumorigenesis of HCC. However, the role and molecular mechanism of potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) in HCC are still unclear. The relative expression of KCNQ1OT1, microRNA-146a-5p (miR-146a-5p) and alkaline ceramidase 3 (ACER3) was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Clonogenic assay was used to assess the radiosensitivity of cells. Cell apoptosis and metastasis were evaluated by flow cytometry and transwell assays, respectively. The protein levels of apoptosis markers, metastasis markers and ACER3 were detected by western blot (WB) analysis. The relationship between miR-146a-5p and KCNQ1OT1 or ACER3 was determined by dual-luciferase reporter assay. Additionally, animal experiments were carried out to explore the effect of KCNQ1OT1 silencing on HCC tumor growth <i>in vivo</i>. KCNQ1OT1 was highly expressed in HCC, and its knockdown hindered the proliferation and metastasis, while increased the radiosensitivity and apoptosis of HCC cells. MiR-146a-5p could interact with KCNQ1OT1, and its inhibition reversed the effects of silenced-KCNQ1OT1 on the radiosensitivity and tumorigenesis of HCC cells. Besides, ACER3 was a target of miR-146a-5p, and its overexpression inversed the effects of miR-146a-5p mimic on the radiosensitivity and tumorigenesis of HCC cells. The expression of ACER3 was regulated by KCNQ1OT1 and miR-146a-5p. Furthermore, KCNQ1OT1 also could reduce the growth of HCC by regulating the miR-146a-5p/ACER3 axis <i>in vivo</i>. Our study suggested that KCNQ1OT1 improved ACER3 expression to regulate the radiosensitivity and tumorigenesis of HCC through sponging miR-146a-5p, indicating that KCNQ1OT1 might be a new therapeutic target for HCC.
DOI: 10.6084/m9.figshare.19780272.v1
title: Cyclophilin A represses reactive oxygen species generation and death of hypoxic non-small-cell lung cancer cells by degrading thioredoxin-interacting protein

description: Cyclophilin A (cypA) is overexpressed in many types of carcinomas, including non-small-cell lung cancer (NSCLC). However, the effect of anoxia, a critical feature of the carcinoma cell microenvironment, on cypA expression in NSCLC is unknown. Here, formaldehyde-fixed and paraffin-embedded samples were collected from 60 subjects with NSCLC. The protein expression levels of cypA and hypoxia-inducible factor-1α (HIF-1α) were evaluated using immunohistochemistry. Kaplan–Meier analysis showed that subjects with high cypA expression had remarkably shorter progression-free survival than those with low cypA expression. Furthermore, cypA expression levels were significantly related to HIF-1α expression levels (Spearman’s correlation=0.34, P&lt;0.0001). To further assess the effect of cypA, an anoxic carcinoma cell model was established. CypA expression was remarkably upregulated in H1299 and A549 cell lines under hypoxic conditions. Overexpression of cypA restored hypoxia-impaired cell growth and prevented reactive oxygen species (ROS) production and cell death in hypoxic A549 and H1299 cells. However, these phenotypes were not altered by the inactive R55A mutant of cypA. Mechanistic studies demonstrated that cypA can bind to and degrade the tumor suppressor protein TXNIP in H1299 and A549 cells. Restored TXNIP expression in cypA-overexpressed and hypoxic NSCLC cells led to increased ROS levels and apoptotic cell numbers and decreased cell growth compared with cypA-overexpressed and hypoxic NSCLC cells. These findings indicate that anoxia results in an increase in cypA expression in NSCLC. Additionally, cypA served as an oncogene during hypoxia by interacting with TXNIP.
DOI: 10.6084/m9.figshare.20296269
title: Additional file 5 of An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome

description: Additional file 5. Table S4. Expression analysis of all E3 ligase encoding genes.
DOI: 10.17632/pms2bvn442.2
title: 130 TEs transposition activity in human cells

description: We verified the transposition activity of 130 DNA transposons in HEK293T and HeLa cell lines, and the puromycin-resistant colony number represents transposition efficiency.
DOI: 10.17632/z6gdb5fr7x.1
title: Fruit_3

description: A multivariate feature fusion dataset that meets the Chinese grading criteria.
The multivariate feature fusion dataset. Specifically, we collected 
information on three types of fruits, namely, Kuril balsam pear, sainfoin, and satsuma orange. In order to ensure the 
broadness and representativeness of the data, for each fruit we 
We randomly collected and videotaped about 150 samples, and also measured fruit diameter, overall fruit 
Fruit diameter, overall appearance, overall defects and weight were measured in detail to ensure that the data were broad and representative.
Detailed measurements of defects and weight were taken to ensure that each sample had detailed data corresponding to it.
DOI: 10.6084/m9.figshare.12964577.v3
title: LncRNA KCNQ1OT1 inhibits the radiosensitivity and promotes the tumorigenesis of hepatocellular carcinoma via the miR-146a-5p/ACER3 axis

description: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death, and radiotherapy is currently one of the main treatments. Long non-coding RNAs (lncRNAs) are associated with the radiosensitivity and tumorigenesis of HCC. However, the role and molecular mechanism of potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) in HCC are still unclear. The relative expression of KCNQ1OT1, microRNA-146a-5p (miR-146a-5p) and alkaline ceramidase 3 (ACER3) was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Clonogenic assay was used to assess the radiosensitivity of cells. Cell apoptosis and metastasis were evaluated by flow cytometry and transwell assays, respectively. The protein levels of apoptosis markers, metastasis markers and ACER3 were detected by western blot (WB) analysis. The relationship between miR-146a-5p and KCNQ1OT1 or ACER3 was determined by dual-luciferase reporter assay. Additionally, animal experiments were carried out to explore the effect of KCNQ1OT1 silencing on HCC tumor growth <i>in vivo</i>. KCNQ1OT1 was highly expressed in HCC, and its knockdown hindered the proliferation and metastasis, while increased the radiosensitivity and apoptosis of HCC cells. MiR-146a-5p could interact with KCNQ1OT1, and its inhibition reversed the effects of silenced-KCNQ1OT1 on the radiosensitivity and tumorigenesis of HCC cells. Besides, ACER3 was a target of miR-146a-5p, and its overexpression inversed the effects of miR-146a-5p mimic on the radiosensitivity and tumorigenesis of HCC cells. The expression of ACER3 was regulated by KCNQ1OT1 and miR-146a-5p. Furthermore, KCNQ1OT1 also could reduce the growth of HCC by regulating the miR-146a-5p/ACER3 axis <i>in vivo</i>. Our study suggested that KCNQ1OT1 improved ACER3 expression to regulate the radiosensitivity and tumorigenesis of HCC through sponging miR-146a-5p, indicating that KCNQ1OT1 might be a new therapeutic target for HCC.
DOI: 10.17632/2wy8bdjwtb.1
title: Changes in CH4 uptake in upland ecosystems following addition of different nitrogen forms: meta-analysis
DOI: 10.6084/m9.figshare.21353324.v1
title: Naringin regulates mitochondrial dynamics to protect against acetaminophen-induced hepatotoxicity by activating the AMPK/Nrf2 signaling pathway in vitro

description: Naringin (Nar) has been reported to exert potential hepatoprotective effects against acetaminophen (APAP)-induced injury. Mitochondrial dysfunction plays an important role in APAP-induced liver injury. However, the protective mechanism of Nar against mitochondrial damage has not been elucidated. Therefore, the aim of this study was to investigate the hepatoprotective effects of Nar against APAP and the possible mechanisms of actions. Primary rat hepatocytes and HepG2 cells were utilized to establish an in vitro model of APAP-induced hepatotoxicity. The effect of APAP and Nar on cell viability was evaluated by a CCK8 assay and detection of the concentrations of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase. The cellular concentrations of biomarkers of oxidative stress were measured by ELISA. The mRNA expression levels of APAP-related phase II enzymes were determined by real-time PCR. The protein levels of Nrf2, phospho (p)-AMPK/AMPK, and biomarkers of mitochondrial dynamics were determined by western blot analysis. The mitochondrial membrane potential (MMP) was measured by high-content analysis and confocal microscopy. JC-1 staining was performed to evaluate mitochondrial depolarization. Nar pretreatment notably prevented the marked APAP-induced hepatocyte injury, increases in oxidative stress marker expression, reductions in the expression of phase II enzymes, significant loss of MMP, mitochondrial depolarization, and mitochondrial fission in vitro. In conclusion, Nar alleviated APAP-induced hepatocyte and mitochondrial injury by activating the AMPK/Nrf2 pathway to reduce oxidative stress in vitro. Applying Nar for the treatment of APAP-induced liver injury might be promising.
DOI: 10.6084/m9.figshare.21353364
title: Characteristic of decreased bacterial diversity in psoriasis

description: ABSTRACT Disturbance of commensal intestinal microbiota is related to chronic inflammatory dermatosis. We analyzed the diversity of the gut microbiota to characterize the biological variation of psoriasis (Ps). Significant differences of gut microbiome profiles were revealed in murine model with psoriasis by sequencing 16S rRNA V3-V4 variable region. Group comparisons included the imiquimod cream (IMQ group, n=8), the imiquimod cream and antibiotics (ATB) (PC+IMQ group, n=8) and the healthy control (CTRL group, n=8). The gut microbiota existed in Ps groups including IMQ group and PC+IMQ group encompassed less diversity than controls, which were attributed to decreased presence of several taxa. The two Ps groups were characterized by significant reduction in firmicutes. In this study, microbiota of psoriasis was defined by an increase presence of Bacteroides. After treated with ATB, we found substantial increase of Lactobacillales but significant decrease of Clostridiales and Coriobacteriales. Relative lower abundance of multiple intestinal bacteria was observed in Ps groups. Although part of genera were concomitantly reduced in both IMQ and PC+IMQ conditions, we discovered the specialty of PC+IMQ group samples was that contained lower abundance of beneficial taxa. Characteristics of gut microbiota profiles in Ps mice were comparable to profiles in patients with Ps, which were related to alteration of specific inflammatory proteins in disease groups but were significantly different from control group. Thus, this study emphasizes the role of intestinal microbiota in the pathogenesis of Ps and provides new insight for investigating association between intestinal microbes and immune inflammation.
DOI: 10.17632/x6p5v74yhb.1
title: A prospective study for reappraisal of the MD-twin score system for the prognosis of monochorionic diamniotic twin babies.

description: Figure 1.
Management flow diagram in the present study of 83 MD-twin cases. FLP: fetoscopic laser photocoagulation, TTTS: twin-twin transfusion syndrome.

Figure 2.
Progression of MD-twin score in the 6 cases with poor outcome.

Figure 3.
(A) ROC curves obtained by the multivariate analysis for core model and their associating variables (BW, AF, delivery week). (B) Enlarged details in the gray-coated area in (A), specifying the corner of ROC curve for core model set at sensitivity of 50% and specificity of 98.7% (arrow).

Figure 4.
(A) Predictions of a poor prognosis in the present study of 83 MD-twin cases using the core model set. (B) Multivariate view separated by the AF discordance and the BW discordance variables. Note the 6 cases (Case A-F) with adverse outcome individually indicated in the graph.
DOI: 10.6084/m9.figshare.21353364.v1
title: Characteristic of decreased bacterial diversity in psoriasis

description: ABSTRACT Disturbance of commensal intestinal microbiota is related to chronic inflammatory dermatosis. We analyzed the diversity of the gut microbiota to characterize the biological variation of psoriasis (Ps). Significant differences of gut microbiome profiles were revealed in murine model with psoriasis by sequencing 16S rRNA V3-V4 variable region. Group comparisons included the imiquimod cream (IMQ group, n=8), the imiquimod cream and antibiotics (ATB) (PC+IMQ group, n=8) and the healthy control (CTRL group, n=8). The gut microbiota existed in Ps groups including IMQ group and PC+IMQ group encompassed less diversity than controls, which were attributed to decreased presence of several taxa. The two Ps groups were characterized by significant reduction in firmicutes. In this study, microbiota of psoriasis was defined by an increase presence of Bacteroides. After treated with ATB, we found substantial increase of Lactobacillales but significant decrease of Clostridiales and Coriobacteriales. Relative lower abundance of multiple intestinal bacteria was observed in Ps groups. Although part of genera were concomitantly reduced in both IMQ and PC+IMQ conditions, we discovered the specialty of PC+IMQ group samples was that contained lower abundance of beneficial taxa. Characteristics of gut microbiota profiles in Ps mice were comparable to profiles in patients with Ps, which were related to alteration of specific inflammatory proteins in disease groups but were significantly different from control group. Thus, this study emphasizes the role of intestinal microbiota in the pathogenesis of Ps and provides new insight for investigating association between intestinal microbes and immune inflammation.
DOI: 10.6084/m9.figshare.21353297
title: Prognostic role of neutrophil-to-lymphocyte ratio to laryngeal squamous cell carcinoma: a meta-analysis

description: Abstract Introduction New evidence suggests that the ratio of neutrophils to lymphocytes is associated with the prognosis of other carcinoma, but the ratio of neutrophils to lymphocytes in laryngeal squamous cell carcinoma remains controversial. Objective The objective of this meta-analysis was to clarify the prognostic effectiveness of the ratio of neutrophils to lymphocytes in laryngeal squamous cell carcinoma. Methods According to the meta-analysis of the free guide, we searched EMBASE, Pubmed, the Cochrane Library databases. The ratio of neutrophils to lymphocytes of laryngeal squamous cell carcinoma patients was evaluated using mean standard vehicle and confidence interval. The overall survival, disease-free survival and progression free survival of patients with laryngeal squamous cell carcinoma were expressed by standard mean carrier method and confidence interval. The risk ratio of 95% confidence interval was used as an evaluation index for patients with laryngeal squamous cell carcinoma. Results Eight studies, including 1780 patients, used a variety of different end values to classify the ratio of neutrophils to lymphocytes (range 1.78-4.0). Among the eight studies that reported risk ratio of the overall survival, the higher median value was 2.72, and 2 of 4 studies reported disease-free survival results. The critical value of ratio of neutrophils to lymphocytes and overall survival deterioration (risk ratio = 1.68, 95% confidence interval 1.43-1.99, p&lt; 0.001), disease-free survival (risk ratio = 2.09, 95% confidence interval 1.62-2.6, p&lt; 0.001) and progression free survival (risk ratio = 1.92, 95% confidence interval 1.75-2.10, p&lt; 0.001) was associated with with laryngeal aquamous cell carcinoma. The ratio of neutrophils to lymphocytes had prognostic value for laryngeal squamous cell carcinoma. Conclusion The results of this meta-analysis showed that the increase of neutrophils to lymphocytes ratio was related to poor prognosis of laryngeal squamous cell carcinoma. The neutrophils to lymphocytes ratio may serve as a cost-effective prognostic biomarker of poor prognosis of laryngeal squamous cell carcinoma. More high-quality prospective trials are needed to assess the practicability of evaluating the ratio of neutrophils to lymphocytes in laryngeal squamous cell carcinoma.