cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.8292659.v1
title: Gas exchanges of melon under water stress in the Submedium region of the São Francisco River Valley

description: ABSTRACT. The current scenario of increased water scarcity is due to climate change and directly affects food production. It is thus necessary to develop strategies to mitigate the impacts of low water availability. Therefore, the goal of the present study is to evaluate the physiological behaviour of melon cultivars under water stress. The experiment was conducted in a protected environment in the experimental Submedium region of the São Francisco River Valley in the period ranging from October to December. In this study, we used the melon cultivars 'Amarelo' and 'Piel de Sapo'. The experiment was conducted in a randomized block design with three replicates that were subdivided into plots, where the plots were comprised of four irrigation rates (50, 75, 100, and 125% of crop evapotranspiration - CET), subplots were comprised of the two melon cultivars, and sub-subplots were comprised of samplings for physiological analyses (15, 30, and 45 days after transplanting). The parameters evaluated were stomatal conductance, transpiration, net photosynthesis, relationship CI/CA, and accumulated dry matter. Water stress reduced the stomatal conductance, transpiration, net photosynthesis, CI/CA, and accumulated dry matter. 'Piel de Sapo' showed a higher photosynthetic adjustment than 'Amarelo' melon due to the gas exchange behaviour of the former, and it was, therefore, more tolerant to water stress.
DOI: 10.6084/m9.figshare.9900368
title: Reserve metabolism of stored and germinated Araucaria angustifolia seeds

description: ABSTRACT. Germination metabolism of recalcitrant seeds of Araucaria angustifolia is activated in storage, which complicates the seeds conservation and utilization. This study aimed to identify the changes in the reserve metabolites of A. angustifolia seeds throughout storage in order to understand the processes of hydrolysis caused by germination metabolism. Mature seeds were harvested in southern Brazil and stored in an ambient environment and cold chamber conditions. Biochemical analyses were performed for embryos and megagametophytes from seeds stored for 0, 15, 30, 45, and 90 days. Due to seeds being in advanced germination in storage, they were evaluated at 90 days in different early developmental categories: I - seeds with mature embryos, II - seeds with embryos showing apparent elongation along the embryonic axis, and III - seeds with root protrusion. Higher contents of carbohydrate, protein, and amino acids were observed in embryos compared to megagametophytes, and these metabolites were decreased after onset of germination, especially in the embryo tissue. Mobilization of metabolites in megagametophytes would probably increase in later stages of germination. It is suggested that such alterations are not due to deterioration of reserve components, but instead are based on seed metabolism, which remains active after harvest with hydrolysis of metabolites providing energy for germination.
DOI: 10.6084/m9.figshare.9900368.v1
title: Reserve metabolism of stored and germinated Araucaria angustifolia seeds

description: ABSTRACT. Germination metabolism of recalcitrant seeds of Araucaria angustifolia is activated in storage, which complicates the seeds conservation and utilization. This study aimed to identify the changes in the reserve metabolites of A. angustifolia seeds throughout storage in order to understand the processes of hydrolysis caused by germination metabolism. Mature seeds were harvested in southern Brazil and stored in an ambient environment and cold chamber conditions. Biochemical analyses were performed for embryos and megagametophytes from seeds stored for 0, 15, 30, 45, and 90 days. Due to seeds being in advanced germination in storage, they were evaluated at 90 days in different early developmental categories: I - seeds with mature embryos, II - seeds with embryos showing apparent elongation along the embryonic axis, and III - seeds with root protrusion. Higher contents of carbohydrate, protein, and amino acids were observed in embryos compared to megagametophytes, and these metabolites were decreased after onset of germination, especially in the embryo tissue. Mobilization of metabolites in megagametophytes would probably increase in later stages of germination. It is suggested that such alterations are not due to deterioration of reserve components, but instead are based on seed metabolism, which remains active after harvest with hydrolysis of metabolites providing energy for germination.
DOI: 10.6084/m9.figshare.9900242
title: Metabolism of carbohydrates in Estilosantes cv. Campo Grande under different systems of cultivation and nitrogen fertilization

description: ABSTRACT. The objective of this study was to verify the activity of invertases in Estilosantes under the influence of cultivation systems and nitrogen fertilization in two seasons. The experiment was conducted in the Xaraés area, where Estilosantes was planted according to a 2 x 2 factorial scheme with two cultivation systems (monoculture and mixed pasture) in the absence or presence of fertilization (75 kg of N ha-1). A randomized block design with four replications was used. The neutral cytosol (Inv-N), vacuole acid (Inv-V) and cell wall acid (Inv-CW) invertase activity and reducing sugar contents in the leaves and stems were evaluated and analyzed by a 5% probability F-test. The interaction between cultivation systems and nitrogen fertilization was significant for Inv-N in the leaves in the summer, such that greater activity occurred in response to the mixed pasture system and nitrogen fertilization. Nitrogen increased the activity of the Inv-N in the stem in both periods, increased the activity of Inv-V in the leaf in the summer, and decreased the activity of Inv-CW in the fall. This cultivation system influenced the activity of Inv-N regardless of the period of the year and did not interfere with the activities of the acid invertases. The invertases did not show regular changes in relation to nitrogen fertilization.
DOI: 10.6084/m9.figshare.9900242.v1
title: Metabolism of carbohydrates in Estilosantes cv. Campo Grande under different systems of cultivation and nitrogen fertilization

description: ABSTRACT. The objective of this study was to verify the activity of invertases in Estilosantes under the influence of cultivation systems and nitrogen fertilization in two seasons. The experiment was conducted in the Xaraés area, where Estilosantes was planted according to a 2 x 2 factorial scheme with two cultivation systems (monoculture and mixed pasture) in the absence or presence of fertilization (75 kg of N ha-1). A randomized block design with four replications was used. The neutral cytosol (Inv-N), vacuole acid (Inv-V) and cell wall acid (Inv-CW) invertase activity and reducing sugar contents in the leaves and stems were evaluated and analyzed by a 5% probability F-test. The interaction between cultivation systems and nitrogen fertilization was significant for Inv-N in the leaves in the summer, such that greater activity occurred in response to the mixed pasture system and nitrogen fertilization. Nitrogen increased the activity of the Inv-N in the stem in both periods, increased the activity of Inv-V in the leaf in the summer, and decreased the activity of Inv-CW in the fall. This cultivation system influenced the activity of Inv-N regardless of the period of the year and did not interfere with the activities of the acid invertases. The invertases did not show regular changes in relation to nitrogen fertilization.
DOI: 10.6084/m9.figshare.9900233
title: Agronomic performance of transgenic soybean cultivars in Brazilian Cerrado

description: ABSTRACT. The productive capacity of soybeans is associated with the adaptation of the culture to the cultivation environment. Factors such as a high number of pods, the weight of a thousand grains, and grain number are essential factors in determining cultivars of high productive potential. The launching of new varieties associated with new technologies makes it necessary to know the performance of these varieties in different growing environments. This study aimed to analyze the agronomic performance of transgenic soybean cultivars under Cerrado conditions. The experimental design used completely randomized blocks; six of the twelve cultivars used RR1 technology and the remaining six used RR2BT. The cultivars were grown in five distinct locations in the southwest region of Goiás. The results demonstrated no increments in the grain yield of the RR1 or RR2BT technologies and the precocity of the cultivars also did not favor an increase in grain yield. The localities of Jataí and Rio Verde did not influence the cultivar cycle.
DOI: 10.6084/m9.figshare.9900233.v1
title: Agronomic performance of transgenic soybean cultivars in Brazilian Cerrado

description: ABSTRACT. The productive capacity of soybeans is associated with the adaptation of the culture to the cultivation environment. Factors such as a high number of pods, the weight of a thousand grains, and grain number are essential factors in determining cultivars of high productive potential. The launching of new varieties associated with new technologies makes it necessary to know the performance of these varieties in different growing environments. This study aimed to analyze the agronomic performance of transgenic soybean cultivars under Cerrado conditions. The experimental design used completely randomized blocks; six of the twelve cultivars used RR1 technology and the remaining six used RR2BT. The cultivars were grown in five distinct locations in the southwest region of Goiás. The results demonstrated no increments in the grain yield of the RR1 or RR2BT technologies and the precocity of the cultivars also did not favor an increase in grain yield. The localities of Jataí and Rio Verde did not influence the cultivar cycle.
DOI: 10.6084/m9.figshare.9900251
title: Plot size for evaluation of Arabica coffee yield

description: ABSTRACT. In most cases, in genetic breeding of Arabica coffee, plot size is defined in an empirical manner. It is often based only on the experience of the breeders and the availability of resources, potentially leading to a reduction in precision. Therefore, the aim of this study was to estimate the size of the experimental plot for evaluation of coffee yield. We evaluated two experiments for validation of cultivars with 12 treatments set up in a randomized complete block design with three replicates and plots composed of 50 plants. Each plant was considered as a basic unit. Estimates of ideal plot size were made by maximum curvature of the coefficient of variation, linear-plateau segmented model and by the resampling methods. We discussed the variations in the parameter estimates for different plot sizes. Divergence was seen among the plot sizes estimated by the different methodologies. Increasing the number of plants per plot led to a higher experimental precision to the point that the increase was no longer significant. The plot size recommended for evaluating coffee production is from seven to 19 plants.
DOI: 10.6084/m9.figshare.9900251.v1
title: Plot size for evaluation of Arabica coffee yield

description: ABSTRACT. In most cases, in genetic breeding of Arabica coffee, plot size is defined in an empirical manner. It is often based only on the experience of the breeders and the availability of resources, potentially leading to a reduction in precision. Therefore, the aim of this study was to estimate the size of the experimental plot for evaluation of coffee yield. We evaluated two experiments for validation of cultivars with 12 treatments set up in a randomized complete block design with three replicates and plots composed of 50 plants. Each plant was considered as a basic unit. Estimates of ideal plot size were made by maximum curvature of the coefficient of variation, linear-plateau segmented model and by the resampling methods. We discussed the variations in the parameter estimates for different plot sizes. Divergence was seen among the plot sizes estimated by the different methodologies. Increasing the number of plants per plot led to a higher experimental precision to the point that the increase was no longer significant. The plot size recommended for evaluating coffee production is from seven to 19 plants.
DOI: 10.6084/m9.figshare.9900236
title: Insecticide resistance and control failure likelihood among populations of the boll weevil (Anthonomus grandis) from Mato Grosso (Brazil)

description: ABSTRACT. The cotton producers from southern Mato Grosso are currently experiencing control failure with the use of the use of insecticides against the cotton boll weevil Anthonomus grandis Boheman, the main pest species of this commodity. Therefore, the present study was designed to survey insecticide resistance and the associated likelihood of control failure among boll weevil populations in the region. Ten insect populations were sampled during the 2016/2017 season and subjected to time-mortality (contact) bioassays in glass vials impregnated with dried insecticide residues at their respective label rates. The three insecticides most frequently used in the region were surveyed: the organophosphate malathion and the pyrethroids beta-cyfluthrin and zeta-cypermethrin. The survival curves showed estimates of the respective median survival time (LT50) for each combination of insecticide and insect copulation. However, there were no significant differences in susceptibility among populations. The estimates of control failure likelihood for each compound at their respective label rates also indicated negligible risk of control failure with their use. These findings are consistent with time-mortality results indicating the lack of insecticide resistant populations at the surveyed sampling sites, suggesting that the reported field control failures result from other causes such as problems with insecticide application.
DOI: 10.6084/m9.figshare.9900236.v1
title: Insecticide resistance and control failure likelihood among populations of the boll weevil (Anthonomus grandis) from Mato Grosso (Brazil)

description: ABSTRACT. The cotton producers from southern Mato Grosso are currently experiencing control failure with the use of the use of insecticides against the cotton boll weevil Anthonomus grandis Boheman, the main pest species of this commodity. Therefore, the present study was designed to survey insecticide resistance and the associated likelihood of control failure among boll weevil populations in the region. Ten insect populations were sampled during the 2016/2017 season and subjected to time-mortality (contact) bioassays in glass vials impregnated with dried insecticide residues at their respective label rates. The three insecticides most frequently used in the region were surveyed: the organophosphate malathion and the pyrethroids beta-cyfluthrin and zeta-cypermethrin. The survival curves showed estimates of the respective median survival time (LT50) for each combination of insecticide and insect copulation. However, there were no significant differences in susceptibility among populations. The estimates of control failure likelihood for each compound at their respective label rates also indicated negligible risk of control failure with their use. These findings are consistent with time-mortality results indicating the lack of insecticide resistant populations at the surveyed sampling sites, suggesting that the reported field control failures result from other causes such as problems with insecticide application.
DOI: 10.6084/m9.figshare.7482269.v1
title: Synergy between cadmium and zinc in bean plants cultivated in multi contaminated soils

description: ABSTRACT. Agricultural species are subjected to a variety of biotic and abiotic stresses, which are the main limitations to crop production. In this context, contamination by trace elements is characterized as an abiotic stress that represents an environmental problem. Due to the physical and chemical similarities between cadmium and zinc, these elements may interact in the environment and may cause antagonistic or synergistic effects. In this way, physiological mechanisms to exclude, detoxify or compartmentalize trace elements that are in excess are crucial for plant survival when exposed to high concentrations of these elements. In this way, the aim of this study was to understand the physiological responses of Phaseolus vulgaris plants subjected to increasing doses of Cd and Zn for 21 days in different soil, Cambisol and Latosol. The activity of antioxidant enzymes, such as SOD, CAT, and APX; hydrogen peroxide content; lipid peroxidation; chlorophyll index; photosynthetic rate; stomatal conductance; and transpiration were analysed. The data obtained showed a specific behaviour of Phaseolus vulgaris plants in each soil analysed. Moreover, it was observed that interactions between both elements resulted in a synergistic effect, negatively affecting all of the parameters analysed.
DOI: 10.6084/m9.figshare.7482269
title: Synergy between cadmium and zinc in bean plants cultivated in multi contaminated soils

description: ABSTRACT. Agricultural species are subjected to a variety of biotic and abiotic stresses, which are the main limitations to crop production. In this context, contamination by trace elements is characterized as an abiotic stress that represents an environmental problem. Due to the physical and chemical similarities between cadmium and zinc, these elements may interact in the environment and may cause antagonistic or synergistic effects. In this way, physiological mechanisms to exclude, detoxify or compartmentalize trace elements that are in excess are crucial for plant survival when exposed to high concentrations of these elements. In this way, the aim of this study was to understand the physiological responses of Phaseolus vulgaris plants subjected to increasing doses of Cd and Zn for 21 days in different soil, Cambisol and Latosol. The activity of antioxidant enzymes, such as SOD, CAT, and APX; hydrogen peroxide content; lipid peroxidation; chlorophyll index; photosynthetic rate; stomatal conductance; and transpiration were analysed. The data obtained showed a specific behaviour of Phaseolus vulgaris plants in each soil analysed. Moreover, it was observed that interactions between both elements resulted in a synergistic effect, negatively affecting all of the parameters analysed.
DOI: 10.6084/m9.figshare.7942052
title: Temporal evaluation of soil chemical attributes after slash-and-burn agriculture in the Western Brazilian Amazon

description: ABSTRACT. In Amazonia, the main causes of deforestation are systems based on slash-and-burn agriculture. The objective of this work was to evaluate temporal changes in the soil chemical attributes after the vegetation has been slashed and burned in an area of native forest in the Western Amazon. For this study, four hectares of forest in Cruzeiro do Sul, State of Acre, Brazil, was slashed and burned. The soil in the study area was classified as Ultisol (Argissolo Amarelo Distrófico típico). Soil samples were collected over the course of one year, the first one being collected 16 hours after the burn and the others at 30, 60, 90, 150, 210, 270, and 360 days after the burn. The soil surface layers were more sensitive to fire action. Ashes from vegetation burn increased pH values, basic cations, and C values only in the first months, resulting in higher basal saturation values (V%). The practice of slash-and-burn after 12 months resulted in lower values of pH, Ca, Mg, K, and P and higher values of Al and H, as well as saturation by aluminum in the soil, indicating this practice contributes to the reduction of soil fertility.
DOI: 10.6084/m9.figshare.7942052.v1
title: Temporal evaluation of soil chemical attributes after slash-and-burn agriculture in the Western Brazilian Amazon

description: ABSTRACT. In Amazonia, the main causes of deforestation are systems based on slash-and-burn agriculture. The objective of this work was to evaluate temporal changes in the soil chemical attributes after the vegetation has been slashed and burned in an area of native forest in the Western Amazon. For this study, four hectares of forest in Cruzeiro do Sul, State of Acre, Brazil, was slashed and burned. The soil in the study area was classified as Ultisol (Argissolo Amarelo Distrófico típico). Soil samples were collected over the course of one year, the first one being collected 16 hours after the burn and the others at 30, 60, 90, 150, 210, 270, and 360 days after the burn. The soil surface layers were more sensitive to fire action. Ashes from vegetation burn increased pH values, basic cations, and C values only in the first months, resulting in higher basal saturation values (V%). The practice of slash-and-burn after 12 months resulted in lower values of pH, Ca, Mg, K, and P and higher values of Al and H, as well as saturation by aluminum in the soil, indicating this practice contributes to the reduction of soil fertility.
DOI: 10.6084/m9.figshare.20012699
title: transcriptomic data

description: BM nucleated cells (BMNCs) from healthy donors and patients with non-severe aplastic anemia at the time of initial diagnosis were separated, and then co-cultured with or without 40 μg/mLLMS for 48 hours in vitro.Cells were harvested and total RNA were extracted. The RNA-sequencing assay were carried out using the Illumina HiSeq X platformand the data obtained were analyzed by following the Hisat2 protocol.
DOI: 10.6084/m9.figshare.20012699.v1
title: transcriptomic data

description: BM nucleated cells (BMNCs) from healthy donors and patients with non-severe aplastic anemia at the time of initial diagnosis were separated, and then co-cultured with or without 40 μg/mLLMS for 48 hours in vitro.Cells were harvested and total RNA were extracted. The RNA-sequencing assay were carried out using the Illumina HiSeq X platformand the data obtained were analyzed by following the Hisat2 protocol.
DOI: 10.6084/m9.figshare.11756934
title: Calibration of reference evapotranspiration models in Pará

description: ABSTRACT. The use of empirical agrometeorological models that can be adjusted to the climatic conditions of different regions has become increasingly necessary to improve water management in grain-producing municipalities. The aim of this work is to examine the correlation between various reference evapotranspiration (ETo) estimation methods and the standard FAO 56 Penman-Monteith method, as well as to determine correction factors, when necessary, for crop-producing municipalities in the northeast of Pará, during both the rainy and dry seasons. We compared simpler methods of ETo estimation to the FAO 56 Penman-Monteith method. For this purpose, meteorological data from Tracuateua, Bragança, Capitão Poço and Castanhal, provided by the National Institute of Meteorology (INMET), were used. The calibration of equations was performed through linear regression. The accuracy of different estimation methods was examined. The Turc, FAO 24 Blaney-Criddle and regression methods presented the best results for all statistical criteria; the Priestley-Taylor, Makkink and FAO 24 Radiation methods presented excellent results after calibration. The methods of Camargo and Hargreaves-Samani produced the worst results for all the criteria.