cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.17632/wjhtzjyy45
title: Blotting analysis of wild type (WT) HaCaT and p53 knockdown HaCaT cells

description: Here we present the experimental data of blotting and immunosorbent analysis of wild type (WT) HaCaT and p53 knockdown HaCaT cells. Provided results were obtained to confirm the knockdown of p53 in HaCaT keratinocytes. 
HaCaT cells were obtained from Bank of DKFZ, Heidelberg and cultured in DMEM/F:12 medium (1:1, Gibco, USA) supplemented with 1% GlutaMAX (Thermo Fisher Scientific, USA), penicillin/streptomycin (100 UI/mL and 100 μg/mL, Gibco, USA) and 10% v/v fetal bovine serum (Gibco, USA). Cells were grown in 60 mm Petri dishes (Corning, USA) and harvested by trypsinization. The culture medium was replaced by fresh every other day.
The knockdown was performed using shRNA designed to knockdown TP53 expression. To perform the knockdown, we applied lentiviral particles (LVPs) encoding anti-TP53 shRNA pLKO-p53-shRNA (Addgene, #25637). To assemble the LVPs, the pLKO-p53-shRNA was delivered in HEK293T cells using PEI transfection. The condition medium was collected 48-72 hours post transfection and purified from cell debris via centrifugation (45 min at 4500g and 4° C).  HaCaT cells were grown until 70% confluence, then the cultivation medium was replaced with condition medium containing LPVs for 1.5 hours. The cells were cultured for 4 days under standard conditions, afterwards the medium was replaced with puromycin-containing medium (1 µg/ml). As a control, we used HaCaT cells transduced with lego-ig2 (Addgene, #27341), which does not contain the puromycin resistance gene. After 10 days of puromycin selection 100% of control cells were dead. The obtained cells were used for further experiments. 

For the immunosorbent assay, the cells were seeded at density of 1×104 cells per well 48 hours prior analysis. ELISA was performed via Abcam’s assay (ab205713) for semi-quantitative p53 determination in accordance with the supplier's manual. 
For blotting analysis, the cells were grown until confluence of 70% at 60 mm Petri dishes under standard conditions. The cells were rinsed with DPBS and lysed in RIPA buffer (150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0). Then 4x Laemmli buffer was mixed with the samples to 1x followed by 10 min incubation at 90C. The proteins were separated in 8% sodium SDS-PAGE and transfered to PVDF membrane. The immunodetection was performed with antibodies to human p53 (clone BP-53-12, PrimeBioMed, Russia) and β-actin (Bio-rad, VMA00048). 
Alkaline phosphatase (AP)-conjugated secondary antibodies (Bio-rad, STAR108A) were used for the immunodetection. The chemiluminescence was detected with DNR LumiBis Gel Imaging System 3.2. The images were obtained using GelCapture software. 
Here we deposit the results of immunoblotting assay confirming the decrease of p53 level in p53 knockdown HaCaT cells. The provided data includes the results of 3 independent experiments. Also, we present the raw data obtained from the ELISA assay.
DOI: 10.6084/m9.figshare.12792491.v1
title: RNA-seq raw data of B16F10 cells.

description: RNA-seq of B16F10 cells. S303, S304, S305 are SH-1, SH-2, SH-3. S306, S307, S308 are CK-1, CK-2, CK-3
DOI: 10.17632/xvwkfymj66
title: Devanagari Vowels dataset( Marathi Vowels Dataset)

description: Devanagari vowel's Handwritten dataset is created and shared herewith
DOI: 10.6084/m9.figshare.13398965.v1
title: Supplementary Material for: An Association Rule Analysis of Combined Acupoints for the Treatment of Patients with Dry Eye Disease

description: <b><i>Background:</i></b> Dry eye disease (DED) has a higher incidence in old age and is seen predominantly in females worldwide. Neurosensory abnormalities, ocular surface inflammation and damage, film instability, and hyperosmolarity are major and proven pathologies responsible for a poor quality of life. Tear breakup time and Schirmer’s I test are predominantly used for the evaluation of primary outcomes in patients undergoing conventional treatment. A previous meta-analysis of some relevant studies proved that combination of acupoints could be more effective than single acupoint treatment. <b><i>Objectives:</i></b> The present study aimed to undertake association rule mining and examined the potential kernel acupoint combination in DED treatment constructed from the extracted randomized controlled trials (RCTs) based on a previous meta-analysis. <b><i>Methods:</i></b> We summarized 32 acupoints as binary data from the 12 eligible RCTs and analyzed them based on the Apriori algorithm. <b><i>Results:</i></b> TE23, BL2, ST2, ST1, EX-HN5, BL1, LI4, ST36, SP6, and KI3 were the 10 most frequently selected acupoints. The major associated rules in combination of acupoints were {TE23, LI4} ≥ {ST1} and {TE23, ST1} ≥ {LI4}, as inferred from 23 association rules. <b><i>Conclusions:</i></b> For acupuncture treatment of DED, combined TE23, LI4, and ST1 acupoints could be settled as the kernel of acupoint combination.
DOI: 10.17632/hdcxctggkk
title: Data for: Approximation of thermodynamic functions of aluminosilicates with zeolite-like compositions by Einstein-Planck functions sum

description: This repository contains the input files (*.txt) for CpFit program. These files are database that contains Einstein-Planck functions sum model parameters and experimental data (thermodynamic properties of individual aluminosilicates) that were used for their optimization. The input files also contain all statistical weights that are required for this optimization. This repository also contains the files (*.csv) with tabulated values. * T is given in K. * Cp (isobaric heat capacity) is given in J/(mol*K). * S (entropy) is given in J/(mol*K). * H-H0 (heat content) is given in J/mol.
DOI: 10.17632/rhs8y2r7jv
title: Data for: Reverse Entrepreneurial Migration in China and India: The Role of the State

description: This includes two original data sets: 1) a China survey of 100 returned and 100 local entrepreneurs from Shanghai, Beijing and Guangzhou conducted in 2004 through a face-to-face format; 2) an India survey administered in 2016-17, comprising 150 returned entrepreneurs and senior managers, and 75 local entrepreneurs in the Indian cities of Pune, Bangalore and New Delhi.
DOI: 10.17632/37vrnb7yxz
title: Scaling a Dpp morphogen gradient through feedback control of receptors and co-receptors. Zhu et al.
DOI: 10.17632/r7b9shhx6p
title: The LC-QTOF-MS/MS analysis of acid degradation products of Rifaximin, An Antibiotic

description: The present research aims to propose a simple and accurate technique for the
analysis of Rifaximin in the presence of its stress degradation products and analysis of
degradation products by LC-MS/MS analysis. Rifaximin was submitted to forced
degradation under acid hydrolysis condition as prescribed by the ICH.The active
Rifaximin was complementary provided by Hilton Pharma Pvt Ltd, and the extract was
prepared by firstly treated with HCl and heated about 4 to 8 hours. The filtrate was
collected and separated using dichloromethane followed by evaporation in rotary
evaporator to obtain a solid crude extract which was then stored under refrigeration at -
80  0  C. Liquid chromatography quadrupole time of ﬂight mass spectrometry (LC-
QTOF-MS/MS) was utilized to identify products in the drug sample. The data
processing results revealed the presence of 9 products in the degraded sample of
Rifaximin. This data article contains the m/z [M+H + ] values, molecular formula,
retention times and the comprehensive list of m/z values detected during the LC-
QTOF- MS/MS analysis.
DOI: 10.17632/cgbmz3hd58
title: Data for: Site specific, high-resolution characterisation of porosity in graphite using FIB-SEM tomography

description: Raw tiff filesof SEM images obtained in this research.
DOI: 10.17632/mkr36ryytw
title: Journal of Iran's Economic Essays; "Jostarhaye Eghtesadiye Iran; No. 29

description: Journal of Economic Essays; an Islamic approach (JEE) is an open-access double-blind peer-reviewed academic quarterly journal that publishes original research, written with an Islamic approach in various Economic areas including Islamic economics and finance, Iran's economy, Fiqh (Islamic jurisprudence) and economics, philosophy of economics, economic theory, ethics and business, Islamic banking, economic development, and social security institution in Islam. JEE especially welcomes comparative articles that study the western and Muslim scholars' viewpoints about important economic issues to find the best answer to theoretical and applied questions and also to explore their differences and similarities with the aim of bringing them closer to each other. This journal accepts analytical articles that reread Muslim scholars' ideas according to new approaches in economics, moral philosophy, Islamic banking and finance, social security, econometrics, and Islamic economic institutions. Therefore, it aims to serve the interests of a wide range of thoughtful and academic readers, economists, philosophers, theologians, banking and finance experts and others interested in the multidisciplinary study of economics and Islam (including Fiqh "Islamic jurisprudence"). We hope that this publication creates an atmosphere of constructive dialogue on different economic topics between national and international scholars by which it could have a role in the growth of Islamic economic thought in Iran. In addition, Islamic solutions to the economic problems of Iran and the world will be examined and analyzed, and they will be presented to the policy makers to help improve the Islamic economic system.

Major Topics

 The Journal publishes papers, including the following fields:

- Islamic economics and finance;

 - Iran's economy;

 - Fiqh (Islamic jurisprudence) and economics;

 - Philosophy of economics;

 - Economic theory;

 - Ethics;

- Islamic economic institutions

 - Islamic banking;

 - Economic development;

- Social security.
DOI: 10.17632/wnskdj8p5s
title: Data fitness little penguin

description: Fitness and life-history traits of little penguins (raw and relative)
DOI: 10.17632/88d7m2fv7p
title: SPHERE: Students' performance dataset of conceptual understanding, scientific ability, and learning attitude in physics education research (PER)

description: The SPHERE is students' performance in physics education research dataset. It is presented as a multi-domain learning dataset of students’ performance on physics that has been collected through several research-based assessments (RBAs) established by the physics education research (PER) community. A total of 497 eleventh-grade students were involved from three large and a small public high school located in a suburban district of a high-populated province in Indonesia. Some variables related to demographics, accessibility to literature resources, and students’ physics identity are also investigated. Some RBAs utilized in this data were selected based on concepts learned by the students in the Indonesian physics curriculum. We commenced the survey of students’ understanding on Newtonian mechanics at the end of the first semester using Force Concept Inventory (FCI) and Force and Motion Conceptual Evaluation (FMCE). In the second semester, we assessed the students’ scientific abilities and learning attitude through Scientific Abilities Assessment Rubrics (SAAR) and the Colorado Learning Attitudes about Science Survey (CLASS) respectively. The conceptual assessments were continued at the second semester measured through Rotational and Rolling Motion Conceptual Survey (RRMCS), Fluid Mechanics Concept Inventory (FMCI), Mechanical Waves Conceptual Survey (MWCS), Thermal Concept Evaluation (TCE), and Survey of Thermodynamic Processes and First and Second Laws (STPFaSL). We expect SPHERE could be a valuable dataset for supporting the advancement of the PER field particularly in quantitative studies. For example, there is a need to help advance research on using machine learning and data mining techniques in PER that might face challenges due to the unavailable dataset for the specific purpose of PER studies. SPHERE can be reused as a students’ performance dataset on physics specifically dedicated for PER scholars which might be willing to implement machine learning techniques in physics education.
DOI: 10.17632/sznjcr462s
title: A program to calculate transverse Breit and QED corrections to energy levels in a multiconfiguration Dirac-Fock environment

description: Title of program: MCBP/BENA Catalogue Id: AAND_v1_0 Nature of problem The program is an extension of, and is used in conjunction with, a multiconfigurational Dirac-Fock (MCDF) program already published. This previous program solved the MCDF equations for an atomic system and dumped the solution (i.e. wavefunctions etc.) to tape or direct access device (hereafter referred to as disk). ... CORRECTION SUMMARY: Vol:Year:Page 23:1981:222 "000A CORRECTION 27/04/81" "A program to calculate transverse Breit and QED corrections to energy levels in a multi-configuration Dirac-Fock environment. (C.P.C. 21(1980)233)." B.J. McKenzie; I.P. Grant; P.H. Norrington Note: correction instructions are contained in source code Versions of this program held in the CPC repository in Mendeley Data AAND_v1_0; MCBP/BENA; 10.1016/0010-4655(80)90042-9 This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2019)
DOI: 10.6084/m9.figshare.7046765.v1
title: Supplementary Material for: R Open Source Programming Code for Calculation of the Kidney Donor Profile Index and Kidney Donor Risk Index

description: <b><i>Background:</i></b> The Kidney Donor Profile Index (KDPI) and Kidney Donor Risk Index (KDRI) were developed by the United States Organ Procurement and Transplantation Network (OPTN). They may influence the clinical decision whether to accept or discard a donor kidney, but still there are debates about KDPI/KDRI applicability and its consequences. To further evaluate these indexes in different populations, more data should be analyzed, and a universally applicable program code would facilitate it. Currently, KDPI/KDRI calculation could be readily done only on the OPTN website that is convenient for a single donor, but not suitable for processing data sets with many records. <b><i>Summary:</i></b> A universally applicable program algorithm in widely used R language for calculating KDPI and KDRI was developed according to donor factors and coefficients described in the OPTN guide. <b><i>Key Messages:</i></b> The open R code permits to calculate KDPI/KDRI either for a single donor or for an unlimited number of records in large data sets. The presented software code would save substantial time to research groups all over the world and help to clarify the KDPI/KDRI role in global settings.
DOI: 10.17632/49vgv5s5t7
title: Evaluating Pleurotus ostreatus Growth for Upcycling Textile Waste from the Fashion Industry

description: The dataset provides detailed experimental data and insights into textile waste management, sustainable material science, and environmental design, supporting circular economy efforts in the fashion industry.
DOI: 10.17632/drhy2y54gm
title: LINF_130013300

description: Protein of unknown function - conserved; Leishmania infantum (strain JPCM5)
DOI: 10.17632/h5ptg7nr54
title: PkSLMNM: Face ROIs

description: This Dataset is extracted from PkSLMNM by finding Face as Region of Interests (ROIs)
DOI: 10.6084/m9.figshare.22811315.v1
title: Supplementary Material for: Soluble CD14 and osteoprotegerin associate with ankle-brachial index as a measure of arterial stiffness in patients with mild to moderate chronic kidney disease in a five-year prospective study.

description: Introduction Vascular lesions and arterial stiffness appear at early stages of chronic kidney disease (CKD) and follow an accelerated course with disease progression, contributing to high cardiovascular mortality. There are limited prospective data on mechanisms contributing to progression of arterial stiffness in mild to moderate CKD (stages 2-3). Methods We applied an affinity proteomics approach to identify candidates of circulating biomarkers with potential impact on vascular lesions in CKD and selected soluble cluster of differentiation 14 (sCD14), angiogenin (ANG) and osteoprotegerin (OPG) for further analysis. We studied their association with Ankle-brachial index (ABI) and carotid intima–media thickness (CIMT), as measures of arteriosclerosis and atherosclerosis respectively, in 48 patients with CKD stages 2-3, who were prospectively followed and intensively treated for five years, and 44 healthy controls. Results Concentrations of both sCD14 (p&lt;0.001), ANG (p&lt;0.001) and OPG (p&lt;0.05) were higher in patients with CKD 2-3 at baseline, and sCD14 (p&lt;0.001) and ANG (p&lt;0.001) remained elevated in CKD patients at follow-up. There were positive correlations between ABI and sCD14 levels (r=0.36, p=0.01) and between ABI and OPG (r=0.31, p=0.03) at five years. The changes in sCD14 during follow-up correlated to changes in ABI from baseline to five years (r=0.41, p=0.004). Conclusion Elevated levels of circulating sCD14 and OPG in patients with CKD 2-3 were significantly associated with ABI, a measure of arterial stiffness. An increase in sCD14 over time in CKD 2-3 patients was associated with a corresponding increase in ABI. Further studies are needed to examine if early intensive multifactorial medication to align with international treatment targets may influence cardiovascular outcomes.
DOI: 10.17632/2rd628xtbx
title: Reliability and Maintenance Data Set for Resilience Optimization

description: This is a dataset with component reliability data, maintenance data, repaircrew data, routing distances for the following paper submitted to RESS for potential publication.
Ryan O’Neil, Claver Diallo, Abdelhakim Khatab, Nidhal Rezg (2024). Enhancing Critical Network Infrastructure Resilience Through Optimal Post-Disruption Maintenance and Routing Decisions, submitted to Reliability Engineering and System Safety, Elsevier.

This data is for experiment 4 in the paper. This set of experiments considers the network displayed in Figure 18, which includes a known source node s, sink node t, and multiple transshipment nodes. The arc index and capacity (in cubic meters per day, CMD) are indicated on each network arc. The network comprises a total of 31 components/arcs. When all components are functioning, the maximum achievable network flow is 112 CMD. With a demand set at d = 112 CMD, there are S = 43 d-MCs.
DOI: 10.17632/dpypk6dtwb
title: Insomnia in the elderly

description: All available treatments of insomnia in the elderly
DOI: 10.17632/ms2jct5wf7
title: Trade with Chinese Characteristics - Gravity Model on Chinese Imports

description: These data files relate to a paper "Trade with Chinese Characteristics - Economics Versus Politics", which applies an augmented gravity model to tease out drivers of China’s bilateral trade relationships, asking why some countries are more important than others as sources of Chinese imports. Our results show that both business and political considerations drive China’s import decisions. Results suggest that established trade agreements have a positive impact on a country's exports to China. Meanwhile, differing political views with China, as measured by UN General Assembly voting records, or having official diplomatic ties with Taiwan result in lesser exports to China. Membership in China’s Belt and Road Initiative, however, does not generally translate into a significant increase in Chinese imports from the member country. The dataset, which covers the period from 2002 to 2022, includes 185 countries counting China. The data are compiled from various sources, such as the UN, World Bank, NATO, American Enterprise Institute, etc.
DOI: 10.17632/zdhkbz6x2d
title: Wind Acceptance Research (WAR) Systematic Review Data

description: The number of studies examining social and community acceptance of wind energy in the US and Canada has increased considerably since the late 1980s. The data archived here contributed to a methodological review of this wind acceptance research (WAR) literature, particularly in response to three articles previously published in Energy Research & Social Science. These include a recent synthesis of WAR by Joe Rand and Ben Hoen (2017) recommending better incorporation of WAR results into development practices and comparability of WAR case studies; an investigation by Kathryn Walsh (2020) and colleagues into potential research fatigue in the related field of unconventional oil and gas development research, and finally a call by Benjamin Sovacool and others (2014, 2018) to increase the theoretical depth and reflection in energy social science. Using a systematic review of 111 WAR articles and a follow-up online survey of 40 corresponding authors, we investigated the location of WAR study sites in the US and Canada, the variety and success of different WAR designs and incentives used, the disciplines and theories dominating current WAR, and finally dissemination practices. Our results suggest that research fatigue is unlikely, yet WAR remains concentrated and in locations distant from the highest installed capacity, and focuses on communities and projects that tend to be novel, controversial, or unique to a specific region. We also find that most WAR lacks an underlying theory, and we conclude by recommending greater qualitative analysis of study site selection criteria, greater integration of existing WAR theories, and greater integration of WAR with solar acceptance research. Finally, we urge wind acceptance researchers to ensure and communicate a clear purpose, value and financial benefit to WAR participants and stakeholders and meaningfully consider the broader community contexts examined.
This dataset only includes information obtained via the systematic review. It does not, per IRB guidance, include results from the Corresponding Author Survey.
DOI: 10.17632/dmkbfdyvhv
title: The effects of androgens on scar formation: a systematic review

description: Supplementary Materials - The effects of androgens on scar formation: a systematic review
DOI: 10.17632/ztcngpwt84
title: DEPRESIÓN Y ANSIEDAD EN MIGRANTES VENEZOLANOS EN EL DEPARTAMENTO ATLÁNTICO

description: El proceso migratorio trae consigo diferentes sentimientos como la ansiedad y la depresión. La ansiedad es una emoción que experimenta el individuo cuando se siente con miedo o preocupación ante situaciones cotidianas, mientras que la depresión es considerada como un trastorno mental en la que persiste la tristeza y la perdida de interés en el día a día, conllevando a la afectación del estado físico y mental, expresados por medio de los pensamientos y comportamientos. El estudio es de tipo observacional descriptivo transversal. La población del estudio está conformada por todos los migrantes venezolanos que se encuentran registrados en la fundación “Fundemar” en un municipio del departamento del atlántico, y la muestra fue tomada en su totalidad. Se aplicaron dos encuestas: test de Hamilton para ansiedad y el test de Hamilton de 17 ítems para depresión. Se realizó un análisis descriptivo de los datos obtenidos de la aplicación de las encuestas donde se obtuvo que el 76% de las personas encuestadas presentaron ansiedad leve y sólo el 6% tuvo ansiedad severa. El 58% de los migrantes que participaron en el estudio no presentan depresión, el 30% presenta depresión ligera menor, el 8% depresión moderada y el 2% depresión severa. Los resultados revelaron un panorama significativo, la mayoría de los participantes mostraron niveles de ansiedad clasificados como leves, mientras que una proporción mínima experimentó ansiedad severa.
DOI: 10.17632/d3wdzgz88s
title: Software Development Analytics in Practice : A Systematic Literature Review

description: Software Development Analytics in Practice : A Systematic Literature Review
DOI: 10.17632/jky24ffbx5
title: data in brief

description: The dataset of the manuscript, namely "Protocol for the development process of ANN models and its application to water quality variables prediction in water resource and environmental engineering".