DOI: 10.5061/dryad.5hqbzkh2g
title: Alternative reproductive tactics in male freshwater fish influence the accuracy of species recognition

description: Sexual conflict can result in coercive mating. Because males bear low
 costs of heterospecific mating, coercive males may engage in misdirected
 mating attempts toward heterospecific females. In contrast, sexual
 selection through consensual mate choice can cause mate recognition cues
 among species to diverge, leading to more accurate species recognition.
 Some species show both coercive mating and mate choice–associated
 courtship behaviors as male alternative reproductive tactics. We
 hypothesized that if the selection pressures on each tactic differ, then
 the accuracy of species recognition would also change depending on the
 mating tactic adopted. We tested this hypothesis in the guppy (Poecilia
 reticulata) and mosquitofish (Gambusia affinis) by a series of choice
 experiments. Poecilia reticulata and G. affinis males both showed
 imperfect species recognition and directed all components of mating
 behavior towards heterospecific females. They tended to direct courtship
 displays more frequently towards conspecific than heterospecific females.
 With male P. reticulata, however, accurate species recognition disappeared
 when they attempted coercive copulation: they directed coercions more
 frequently towards heterospecific females. We also found that
 heterospecific sexual interaction had little effect on the fecundity of
 gravid females, which suggests that pre-pregnancy interactions likely
 underpin the exclusion of G. affinis by P. reticulata in our region.
description: exp1_maleMateChoice ------ exp1_maleMateChoiceDataset.csv
 (2019/07/23): Male association preference for con- and heterospecific
 females.<br>                        |-- exp1_1_summaryResult.R:
 Average, Standard deviation, and paired t test for male association
 preference.<br>                        |-- exp1_2_dirichletReg.R:
 Effect of body size estimated using Dirichlet regression
 exp2_matingBehavior ------ maleIDandBehavior.csv (2019/05/04):
 Frequency of mating behaviors for con- and heterospecific
 females<br>                        |--
 2_1_behaviorFreqeuncy_overdispersion.R: Overdispersion test for each
 behavior (Table S1)<br>                        |--
 2_2_Fig2_behaviorFreqeuncy.R: Boxplot for each behavior<br>        
                |-- 2_3_testEachBehavior.R: Wald tests for differences in
 behavioral frequency between male and female combinations<br>      
                  |-- FigS4_behaviorTransitionNetwork.R: Illustrate the
 behavior transition network exp3_fecundity ------
 exp3_fecundityDataset.csv (2019/01/18): Male effect on female
 fecundity<br>                   |--
 3_1_fecundityExperimentSummary.R: Summary of experiment (Table
 S2) <br>                   |-- 3_1_fecundityExperimentSummary.R:
 Estimation of size-fecundity relationship using Poisson
 regression
description: Users are free to use the database and its content in new and
 different ways,<br> provided they provide attribution to the source
 of the data and/or the database. #
 --------------------------------------------------------------------------------------------------------------<br> # Methodological information: (reference)<br> # --------------------------------------------------------------------------------------------------------------<br> Description of methods for data collection or generation: https://doi.org/10.1002/ece3.7267<br> The R scripts were written and performed using R version 3.3.2 for Windows (R Core Team, 2016, www.r-project.org/). # --------------------------------------------------------------------------------------------------------------<br> # Data-specific information<br> # --------------------------------------------------------------------------------------------------------------<br> exp1_maleMateChoiceDataset.csv (2019/07/23)<br>                        |-- femaleSpecies: The category is where males spent the time.<br>                        |-- maleSpecies: Male species used in the trial.<br>                        |-- maleID: Male individual identifier used in the trial.<br>                        |-- treatment: In the "Acclimation", we showed only conspecific female, while in the "preference", we showed conspecific and heterospecific females.<br>                        |-- spendingTime: Total time spent in the area, the unit is second.<br>                        |-- maleWeight: Male wet body weight, the unit is gram.<br>                        |-- averageFemaleWeight: average female body weight, the unit is gram.<br>                        |-- femaleWeight_poMinusga: female weight difference defined Poecilia female minus Gambusia female, the unit is gram.<br>                        |-- po_femalePerga_female: female weight ratio defined by dividing Poecilia female by Gambusia female.<br>                        |-- exclude: Male individuals excluded from statistical analysis, 1: exlude; 0:include. exp2_matingBehavior ------ maleIDandBehavior.csv (2019/05/04)<br>                        |-- maleID:  Male individual identifier used in the experiment.<br>                        |-- male_species: Male species used in the trial.<br>                        |-- female_species: Female species used in the trial.<br>                        |-- combination: Conspecific or Heterospecific pair in the trial.<br>                        |-- follow: The total counts of "follow" behavior in 20 minutes.<br>                        |-- gonopodial_swing: The total counts of "gonopodial swing" behavior in 20 minutes.<br>                        |-- gonopodial_thrust: The total counts of "gonopodial thrust" behavior in 20 minutes.<br>                        |-- jolt:  The total counts of "jolts" behavior in 20 minutes.<br>                        |-- sigmoid_display:  The total counts of "sigmoid display" behavior in 20 minutes.<br>                        |-- male_SL_mm: Male standard length, the unit is mm.<br>                        |-- female_SL_mm:  Female standard length, the unit is mm.<br>                        |-- male_BW: Male body weight, the unit is gram.<br>                        |-- female_BW: Female body weight, the unit is gram. exp3_fecundity ------ exp3_fecundityDataset.csv (2019/01/18)<br>                   |-- experiment_No: Trial identifier used in the experiment.<br>                   |-- femaleSpecies: Female species used in the trial.<br>                   |-- maleSpecies: Male species used in the trial. "control" means absence of male.<br>                   |-- sex: the sex of males in the trial.<br>                   |-- releaseDay: On the date of giving birth, we continued for up to 32 days.<br>                   |-- larvaeNumber: The number of offspring produced by gravid P. reticulata and G. affinis females<br>                   |-- femaleSL: Female standard length, the unit is mm.