DOI: 10.5061/dryad.2v6wwpzvn
title: Data from: Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance

description: A fundamental issue in the metabolic field is whether it is possible to
 understand underlying mechanisms that characterize individual variation.
 Whole-animal performance relies on mitochondrial function as it produces
 energy for cellular processes. However, individual variation in
 mitochondrial traits lack of longitudinal measures to evaluate how
 mitochondrial function can change within and among individuals and with
 environmental context. The aims of this study were to test the
 repeatability of muscle mitochondrial metabolism thanks to two biopsies
 performed in red muscle, and to evaluate effects of biopsies on
 whole-animal performance in goldfish Carassius auratus. Our results show
 that basal mitochondrial respiration and net phosphorylation efficiency
 are repeatable at 14-day intervals. We also show that swimming performance
 (optimal cost of transport and critical swimming speed) was repeatable in
 biopsied fish, whereas the repeatability of individual oxygen consumption
 (standard and maximal metabolic rates) seemed not stable over time.
 However, we noted that the means of individual and mitochondrial traits
 did not change over time in biopsied fish. This study shows that muscle
 biopsies allow the measurements of mitochondrial metabolism without
 sacrificing animals and that two muscle biopsies 14 days apart affect the
 intraspecific variation in fish performance without affecting average
 performance of individuals.
description: # Data from: Non-lethal sampling for assessment of mitochondrial function
 does not affect metabolic rate and swimming performance
 [https://doi.org/10.5061/dryad.2v6wwpzvn](https://doi.org/10.5061/dryad.2v6wwpzvn) This file includes different documents related to the article "Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance", dealing with the validation of the muscle biopsy method, on 12 cm goldfish. In particular: "ESM 1_Supplemental Data" contains supplemental tables and figures to give more details about the experimental protocols as well as about preliminary measurements to validate the muscle biopsy method. "ESM2 Thoral et al Dataset" contains all the data presented in the manuscript as well as the data presented in "ESM 1Supplemental Data". * The sheet "Whole animal performance traits" contains data presented in Figure 3 and Table 1. The column "Treatment" contains the 2 groups : control fish and biopsied fish (Biopsied MS Lidocaine; LIDO). "Event_number" shows if the data  were obtained during the first or the second trials to measure whole animal performance. "Individual" is the identification name of each individual.  The reason why there are empty cells  is detailed in the column "Comment". All the other columns are data obtained on fish : SMR (standard metabolic rate), MMR (maximum metabolic rate), Ucrit (critical swimming speed), and OptCOT (optimal cost of transport). * The sheet "Sample technique comparison" contains the mitochondrial traits measured on the 12 same fish (TEST) with two different techniques to sample muscle: classical dissection ("Dissection") and biopsy ("Punch"). These data are presented in ESM1 in paragraph 4. All the mitochondrial traits are presented in in the column "Mitochondrial trait", with Basal respiration, OXPHOS respiration, LEAK respiration and cytochrome c respiration. * The sheet "Repeatability mitochondria RptR" corresponds to the data used to measure the repeatability of mitochondrial traits between biopsy 1 and 2 thanks to the RptR function. These data are presented in Figure 2 and in paragraphs 3, 4 and 5 in ESM 1. Three different groups are presented on this table: "Biopsied MS Lidocaine" fish (LIDO) that correspond to the biopsied fish presented in the manuscript; "Biopsied MS" fish that are compared to "Biopsied MS Lidocaine" fish (MS) in ESM1 in paragraph 3; and "Sample technique comparison" fish (TEST) used to compared both sampling techniques (see above). RCR = respiratory control ratio; Net phosphorylation efficiency = 1 - (LEAK respiration / OXPHOS respiration). The body mass of fish from the group "TEST" has not been recorded.  * The sheet "Repeatability mitochondria ICC" corresponds to the data used to measure the repeatability of mitochondrial traits between biopsy 1 and 2 thanks to the ICC function, used for the "Biopsied MS Lidocaine" fish (LIDO) presented in Figure 2. One column of each parameter has been measured during biopsy 1 or biopsy 2 (example: "Basal_respiration_B1" has been measured during biopsy 1 and "Basal_respiration_B2" has been measured during biopsy 2). * The sheet "Correlation individual mitochon" contains the data used to measure the correlation levels between individual and metabolic traits. These correlations are presented in ESM 1, Table S2. Whole-animal performance traits obtained during trial 2 (2 in "Individual event number" column) have been compared to mitochondrial traits obtained during biopsy 1 (1 in "Mitochondria event number") and whole-animal performance traits obtained during trial 3 have been compared to mitochondrial traits obtained during biopsy 2. * The sheet "Biometrie values" contains data obtained during biometries, i.e. body mass, body length and Fulton index (calculated as 100 x (body mass / body length^3). These data have been measured on control fish and biopsied fish before the measurement of whole-animal performance traits (trials 1, 2 and 3 in the column "Event_number"). These data are presented in Table 1 (except body length that has been only used to calculate the Fulton index). "ESM 3_Biometries_values" contains the code used to analyse all the data related to biometries. "ESM 4_Correlation_individual_mitochondrial_traits" contains the code used to analyse the correlation between individual and mitochondrial traits. "ESM 5_Whole_animal_performance\_ and_mitochondrial_traits" contains the code used to analyse all the data related to whole-animal performance as well as mitochondrial traits.