DOI: 10.5061/dryad.9w0vt4btt
title: Molecular species delimitation of Sphagnum subgenus Subsecunda in Europe

description: A taxonomic revision of Sphagnum subgenus Subsecunda in Europe is
 warranted as the taxonomic status and the relationships among the six
 species found in Europe are inconsistent. Here we provide molecular data
 from a large specimen set of species in Sphagnum subgenus Subsecunda
 obtained from European herbaria, used to investigate taxonomic
 relationships in European Subsecunda species. The molecular data
 includes a dataset of 15 microsatellite markers from a total of 310
 specimens and short-nucleotide repeats (SNP) data from 64 specimens
 obtained using double digest restriction-site associated DNA (ddRAD)
 sequencing method. The species included in the datasets are S. contortum,
 S. platyphyllum, S. subsecundum, S. inundatum, and S.
 denticulatum, in addition to one deviating specimen of S. auriculatum from
 the Azores potentially representing an undescribed taxon of subgenus
 Subsecunda in Europe. The methods used to obtain the molecular data are
 described in detail in Meleshko et al.
description: # Molecular species delimitation of Sphagnum subgenus Subsecunda in
 Europe. Access this data on Dryad
 [https://doi.org/10.5061/dryad.9w0vt4btt](https://doi.org/10.5061/dryad.9w0vt4btt) This data was generated to investigate taxonomic issues of species in Sphagnum subgenus Subsecunda. The data were obtained from herbaria in Europe, and the datasets contains data from five species oof Sphagnum subgenus Subsecunda. All specimens are from Europe. DNA was extracted from dried capitula of herbaria specimens. We obtained two kind of molecular data: short nucleotide polymorphisms (SNP) data and microsatellite (short sequence repeats, SSR) data. The former were obtained using double digest RAD sequencing. Detailed methods to obtain molecular data are described in Meleshko et al. 2025 (Botanical Journal of Linneaen Society). ## Description of the data and file structure The data are divided in separate files and names and content are described in detail below. File 1 contains the voucher data of all specimens and should be linked to the ID used in the other files. The first column in the file has the ID of the specimens with SSR data and the second column has the ID for the specimens with SNP data. There are fewer specimens with SNP data than SSR data and specimens with no SNP ID are indicated with n/a. Likewise, specimens in the outgroup with SNP data, are denoted with n/a in the ID column of SSR specimens. File 1 Name: Voucher_data.csv CSV file containing the voucher table with information about all samples: the ID used for the SSR markers, ID used for SNPs, species name, ID of herbarium sample (TRH (herbarium)), the country and site the specimen were collected, the year the specimen were collected and longitude and latitude of the collection. This data gives the background data for the specimens in files 2-5. File 2 Name: SNP_dataset.vcf Variant Call Format file containing the SNP data for 64 specimens (referred to as "SNP dataset" in Meleshko et al. 2025). File 3 Name: Pruned_SNP_dataset.vcf Variant Call Format file containing the pruned SNP data for 64 specimens (referred to as "pruned SNP dataset" in Meleshko et al. 2025). File 4 Name: popmap_SNP.tsv Assignment of individuals from the SNP dataset to species. The file should be combined with the data in files 2 and 3. First column shows the sample ID, and second column gives the species name (genus name - Sphagnum - is not included in the name a all specimens belong to Sphagnum; auriculatum refers to the haploid sample of S. auriculatum). File 5 Name: SSR_dataset.csv SSR data includes SSR ID, name of the species and all SSR markers (15 in total) identified with SSR name (a number corresponding to the length of the DNA fragment, marker names have an added \_1 or \_2 corresponding to allele 1 and allele 2 at the same marker). Each line in the table represents one specimen. As two of the species are diploid, but three are haploid, all specimens are listed as diploid (two alleles per marker). The three haploid species have their alleles duplicated in the list (homozygous). Missing data is given as '0'.