DOI: 10.22002/5s6h2-ssq31
title: MreB-GFP o/e

description: Raw data files of MreB-GFP o/e
description: <strong>Tilt Series Date:</strong> 2007-04-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 195.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strains constructed by Maria Sandkvist (U. Michigan).  Data collected and processed by Zhuo Li (Caltech).</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Strains constructed by Maria Sandkvist (U. Michigan).  Data collected and processed by Zhuo Li (Caltech).</p>
  zla2007-04-21-5_slicer9375.jpg: Vibrio cholerae .  filament bundle zla2007-04-21-5_slicer9376.jpg: Vibrio cholerae .  pili zla2007-04-21-5_slicer9377.jpg: Vibrio cholerae .  filament zla2007-04-21-5_slicer9378.jpg: Vibrio cholerae .  filament bundle zla2007-04-21-5_slicer9379.jpg: Vibrio cholerae .  comb
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2007-04-21-5/3dimage_1418/</p>vcmg4210765w1dot5d8781cts18k12u001_full.rec,  Reconstruction (Pixel Size 2.518 nm), 4.2 GB  
        
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        vcmg4210765w1dot5d8781cts18k12u001.mrc,  Tilt Series (Pixel Size 1.259 nm), 738.3 MB  
        
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        dnvcmg4210765w1dot5d8781cts18k12u001_full.rec,  Reconstruction (Pixel Size 2.518 nm), 7.5 GB  
        
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        vcmg4210765w1dot5d8781cts18k12u001bin_full.rec,  Reconstruction (Pixel Size 2.518 nm), 713.0 MB  
        
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        vcmg4210765w1dot5d8781cts18k12u001bintrim.rec,  Reconstruction (Pixel Size 2.518 nm), 192.3 MB  
        
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        zl2007-04-21-5_clip01.rec,  Reconstruction (Pixel Size 2.518 nm) cropped by Sarah, 147.1 MB  
        
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        vcmg4210765w1dot5d8781cts18k12u001bintrim_cyto.am,  Cytoplasm segmentation, 1.7 MB  
        
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        zl2007-04-21-5ribosegnew.am,  Ribosome segmentation.am file created from post-processed Molmatch data.Created by David J. Rosenman., 3.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament01.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament02.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament03.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament04.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament05.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament06.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament07.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament08.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament09.am,  filament segmented by Sarah, 1.2 MB  
        
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        zl2007-04-21-5_clip01-label-Filament10.am,  filament segmented by Sarah, 1.2 MB  
        
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