cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14749483.v1
title: <i>Garcinia cambogia</i> attenuates adipogenesis by affecting CEBPB and SQSTM1/p62-mediated selective autophagic degradation of KLF3 through RPS6KA1 and STAT3 suppression

description: The overexpansion of adipose tissues leads to obesity and eventually results in metabolic disorders. <i>Garcinia cambogia</i> (<i>G. cambogia</i>) has been used as an antiobesity supplement. However, the molecular mechanisms underlying the effects of <i>G. cambogia</i> on cellular processes have yet to be fully understood. Here, we discovered that <i>G. cambogia</i> attenuated the expression of CEBPB (CCAAT/enhancer binding protein (C/EBP), beta), an important adipogenic factor, suppressing its transcription in differentiated cells. In addition, <i>G. cambogia</i> inhibited macroautophagic/autophagic flux by decreasing autophagy-related gene expression and autophagosome formation. Notably, <i>G. cambogia</i> markedly elevated the expression of KLF3 (Kruppel-like factor 3 (basic)), a negative regulator of adipogenesis, by reducing SQSTM1/p62-mediated selective autophagic degradation. Furthermore, increased KLF3 induced by <i>G. cambogia</i> interacted with CTBP2 (C-terminal binding protein 2) to form a transcriptional repressor complex and inhibited <i>Cebpa</i> and <i>Pparg</i> transcription. Importantly, we found that RPS6KA1 and STAT3 were involved in the <i>G. cambogia</i>-mediated regulation of CEBPB and autophagic flux. In an obese animal model, <i>G. cambogia</i> reduced high-fat diet (HFD)-induced obesity by suppressing epididymal and inguinal subcutaneous white adipose tissue mass and adipocyte size, which were attributed to the regulation of targets that had been consistently identified <i>in vitro</i>. These findings provide new insight into the mechanism of <i>G. cambogia</i>-mediated regulation of adipogenesis and suggest molecular links to therapeutic targets for the treatment of obesity. <b>Abbreviations:</b> 3-MA: 3-methyladenine; ACTB: actin beta; ATG: autophagy-related; Baf: bafilomycin A<sub>1</sub>; BECN1: beclin 1; CEBP: CCAAT/enhancer binding protein (C/EBP); CHX: cycloheximide; CREB: cAMP response element binding protein; CTBP: C-terminal binding protein; EGCG: (-)-epigallocatechin gallate; eWAT: epididymal white; <i>G. cambogia: Garcinia cambogia</i>; GFP: green fluorescent protein; H&E: hematoxylin and eosin; HFD: high-fat diet; iWAT: inguinal subcutaneous white; KLF: Kruppel-like factor; LAP: liver-enriched transcriptional activating proteins; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; ND: normal diet; PPARG: peroxisome proliferator activated receptor gamma; qPCR: quantitative real-time PCR; RFP: red fluorescent protein; RPS6KA1: ribosomal protein S6 kinase A1; siRNA: small-interfering RNA; SQSTM1/p62: sequestosome 1; STAT: signal transducer and activator of transcription; TEM: transmission electron microscopy
DOI: 10.6084/m9.figshare.14714909.v1
title: Silencing PEX26 as an Unconventional Mode to Kill Drug-Resistant Cancer Cells and Forestall Drug Resistance

description: Promoting the macroautophagy/autophagy-mediated degradation of specific proteins and organelles can potentially be utilized to induce apoptosis in cancer cells or sensitize tumor cells to therapy. To examine this concept, we enriched for autophagosomes from histone deacetylase inhibitor (HDACi)-sensitive U937 lymphoma cells and isogenic HDACi-resistant cells. Mass spectrometry on autophagosome-enriched fractions revealed that HDACi-resistant cells undergo elevated pexophagy, or autophagy of the peroxisome, an organelle that supports tumor growth. To disturb peroxisome homeostasis, we enhanced pexophagy in HDACi-resistant cells via genetic silencing of peroxisome exportomer complex components (<i>PEX1, PEX6</i>, or <i>PEX26</i>). This consequently sensitized resistant cells to HDACi-mediated apoptosis, which was rescued by inhibiting ATM/ataxia-telangiectasia mutated (ATM serine/threonine kinase), a mediator of pexophagy. We subsequently engineered melanoma cells to stably repress <i>PEX26</i> using CRISPR interference (CRISPRi). Melanoma cells with repressed <i>PEX26</i> expression showed evidence of both increased pexophagy and peroxisomal matrix protein import defects versus single guide scrambled (<i>sgSCR)</i> controls. <i>In vivo</i> studies showed that <i>sgPEX26</i> melanoma xenografts recurred less compared to <i>sgSCR</i> xenografts, following the development of resistance to mitogen-activated protein kinase (MAPK)-targeted therapy. Finally, prognostic analysis of publicly available datasets showed that low expression levels of <i>PEX26, PEX6</i> and <i>MTOR</i>, were significantly associated with prolonged patient survival in lymphoma, lung adenocarcinoma and melanoma cohorts. Our work highlighted that drugs designed to disrupt peroxisome homeostasis may serve as unconventional therapies to combat therapy resistance in cancer.
DOI: 10.6084/m9.figshare.14790045
title: Activation of microlipophagy during early infection of insect hosts by <i>Metarhizium robertsii</i>

description: The requirement of macroautophagic/autophagic machinery for filamentous fungal development and pathogenicity has been recognized, but the underlying effects and mechanisms remain elusive. The insect pathogenic fungus <i>Metarhizium robertsii</i> infects hosts by cuticular penetration through the formation of the infection structure appressoria. Here, we show that autophagic fluxes were highly activated during the appressorial formation of <i>M. robertsii</i>. Genome-wide deletion of the autophagy-related genes and insect bioassays identified 10 of 23 encoded <i>MrATG</i> genes with requirements for topical fungal infection of insect hosts. Besides the defect in forming appressoria on insects (two null mutants), these virulence-reduced mutants were largely impaired in penetrating cellophane membrane and insect cuticles, suggesting their failures in generating proper appressorium turgor. We found that the conidial storage of lipid droplets (LDs) had no obvious difference between strains, but autophagic LD degradation was impaired in different mutants. After induction of cell autophagy by nitrogen starvation, we found that LD entry into vacuoles was unaffected in the selected mutant cells with potential failures in forming autophagosomes. The finding therefore reveals a microlipophagy machinery employed in this fungus and that the direct engulfment of LDs occurs without inhibition by the downstream defective lipolysis. Our data first unveil the activation and contribution of microlipophagy to fungal infection biology. The obtained technique may benefit future detection of microlipophagy in different organisms by examining vacuolar or lysosomal engulfment of LDs in core autophagic gene deletion mutants. <b>Abbreviations</b>: AIM: Atg8-family interacting motif; ATG: autophagy-related; CM: complete medium; CMAC: 7-amino-4-chloromethylcoumarin; DTT: dithiothreitol; ER: endoplasmic reticulum; GFP: green fluorescent protein; LD, lipid droplet; MM: minimum medium; MM-N: minimum medium without nitrogen source; PDA: potato dextrose agar; PMSF: phenylmethylsulfonyl fluoride; RFP: red fluorescent protein; SDB: Sabouraud dextrose broth; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TAG: triacylglycerol; TEM: transmission electron microscopy; WT, wild type.
DOI: 10.6084/m9.figshare.14790045.v1
title: Activation of microlipophagy during early infection of insect hosts by <i>Metarhizium robertsii</i>

description: The requirement of macroautophagic/autophagic machinery for filamentous fungal development and pathogenicity has been recognized, but the underlying effects and mechanisms remain elusive. The insect pathogenic fungus <i>Metarhizium robertsii</i> infects hosts by cuticular penetration through the formation of the infection structure appressoria. Here, we show that autophagic fluxes were highly activated during the appressorial formation of <i>M. robertsii</i>. Genome-wide deletion of the autophagy-related genes and insect bioassays identified 10 of 23 encoded <i>MrATG</i> genes with requirements for topical fungal infection of insect hosts. Besides the defect in forming appressoria on insects (two null mutants), these virulence-reduced mutants were largely impaired in penetrating cellophane membrane and insect cuticles, suggesting their failures in generating proper appressorium turgor. We found that the conidial storage of lipid droplets (LDs) had no obvious difference between strains, but autophagic LD degradation was impaired in different mutants. After induction of cell autophagy by nitrogen starvation, we found that LD entry into vacuoles was unaffected in the selected mutant cells with potential failures in forming autophagosomes. The finding therefore reveals a microlipophagy machinery employed in this fungus and that the direct engulfment of LDs occurs without inhibition by the downstream defective lipolysis. Our data first unveil the activation and contribution of microlipophagy to fungal infection biology. The obtained technique may benefit future detection of microlipophagy in different organisms by examining vacuolar or lysosomal engulfment of LDs in core autophagic gene deletion mutants. <b>Abbreviations</b>: AIM: Atg8-family interacting motif; ATG: autophagy-related; CM: complete medium; CMAC: 7-amino-4-chloromethylcoumarin; DTT: dithiothreitol; ER: endoplasmic reticulum; GFP: green fluorescent protein; LD, lipid droplet; MM: minimum medium; MM-N: minimum medium without nitrogen source; PDA: potato dextrose agar; PMSF: phenylmethylsulfonyl fluoride; RFP: red fluorescent protein; SDB: Sabouraud dextrose broth; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TAG: triacylglycerol; TEM: transmission electron microscopy; WT, wild type.
DOI: 10.6084/m9.figshare.14922750
title: RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging

description: There is increasing evidence that mitophagy, a specialized form of autophagy to degrade and clear long-lived or damaged mitochondria, is impaired in aging and age-related disease. Previous study has demonstrated the obesity-exposed oocytes accumulate and transmit damaged mitochondria due to an inability to activate mitophagy. However, it remains unknown whether mitophagy functions in oocyte and what’s the regulatory mechanism in oocyte aging. In the study, when fully grown oocytes were treated with CCCP, an uncoupling agent to induce mitophagy, we found the activation of the PRKN-mediated mitophagy pathway accompanied the blockage of meiosis at metaphase I stage. Our result then demonstrated its association with the decreased activity of RAB7 and all the observed defects in CCCP treated oocytes could be effectively rescued by microinjection of mRNA encoding active RAB7<sup>Q67L</sup> or treatment with the RAB7 activator ML098. Further study indicated PRKN protein level as a rate-limiting factor to facilitate degradation of RAB7 and its GEF (guanine nucleotide exchange factor) complex CCZ1-MON1 through the ubiquitin-proteasome system. In GV oocytes collected during ovarian aging, we found the age-related increase of PINK1 and PRKN proteins and a significant decrease of RAB7 which resulted in defects of mitophagosome formation and the accumulation of damaged mitochondria. The age-related retardation of female fertility was improved after in vivo treatment of ML098. Thus, RAB7 activity is required to maintain the balance between mitophagy and chromosome stability and RAB7 activator is a good candidate to ameliorate age-related deterioration of oocyte quality. <b>Abbreviations:</b> ATG9: autophagy related 9A; ATP: adenosine triphosphate; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; CCCP: carbonyl cyanide 3-chlorophenylhydrazone; CCZ1: CCZ1 vacuolar protein trafficking and biogenesis associated; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GAPs: GTPase-activating proteins; GEF: guanine nucleotide exchange factor; GV: germinal vesicle; GVBD: germinal vesicle breakdown; LAMP1: lysosomal-associated membrane protein 1; MI: metaphase I stage of meiosis; MII: metaphase II stage of meiosis; Mito: MitoTracker; mtDNA: mitochondrial DNA; MON1: MON1 homolog, secretory trafficking associated; OPTN: optineurin; PINK1: PTEN induced putative kinase 1; PRKN: parkin RBR E3 ubiquitin protein ligase; RAB7: RAB7, member RAS oncogene family; ROS: reactive oxygen species; TEM: transmission electron microscopy; TOMM20/TOM20: translocase of outer mitochondrial membrane 20; TUBB: tubulin, beta; UB: ubiquitin.
DOI: 10.34740/kaggle/dsv/7908327
title: Public Opinion on Climate Change (Updated Daily)
DOI: 10.34740/kaggle/dsv/7908336
title: Balochi Language to English Translation Dataset
DOI: 10.6084/m9.figshare.24781941
title: Searching for inhibitors of SARS-Cov2 papain-like-protease

description: inputs.zip contains YASARA scenes and macros for all simulations and analysesdistances.zip contains the trajectory analysis created by running the md_analyze_xxx.mcr macros contained on distances.zip on the trajectories. Trajectory files have not been uploaded due to their sheer size, but xxx.zip contains PDB files of every complex throughout the molecular dynamics simulations, taken at 2.5 ns intervals (simulation time). Filenames follow the format xxxx_nnn.pdb , where xxx is the ligand name and nnn is equal to simulation_time (in ns) divided by 0.25.
DOI: 10.25318/3710020301-eng
title: Postsecondary graduates who owed money for their education to government-sponsored student loans at graduation, by province of residence at interview, level of study, field of study and sex, inactive

description: Statistics on postsecondary graduates who owed money for their education to government-sponsored student loans at graduation, including debt at graduation and debt at interview, are presented by the province of residence at time of interview, the level of study, the field of study and sex.
DOI: 10.25318/3710020301-fra
title: Diplômés postsecondaires ayant des dettes d'études dans le cadre de prêts étudiants consentis par le gouvernement au moment de l'obtention du diplôme, selon la province de résidence à l'interview, le niveau d'études, le domaine d'études et le sexe, inactif

description: Les statistiques sur les diplômés postsecondaires ayant des dettes d'études dans le cadre de prêts étudiants consentis par le gouvernement au moment de l'obtention du diplôme, y compris la dette au moment de l'obtention du diplôme et la dette au moment de l'interview, sont présentées selon la province de résidence à l'interview, le niveau d'études, le domaine d'études et le sexe.
DOI: 10.5281/zenodo.10850227
title: Pharsalus repandus (Hemiptera: Ricaniidae) with mutualistic interaction with two species of Camponotus
title: Ant and planthopper interactions

description: The ants who were attending the planthoppers was identified as Camponotus rufipes (Fabricius, 1775) and Camponotus crassus Mayr, 1862 (Fig. 2A, D, arrow 2). We noted direct contact and observed the ants employing antennal palpation behavior to stimulate the planthoppers. The ant positions itself just behind the planthopper, delicately and repeatedly touching its wings with the tips of its antennae. In response to this interaction, the planthopper gradually elevates its forewings, although they do not fully open, and the anal margins remain unseparated. Concurrently, a droplet of honeydew is released. Additionally, we observed the planthoppers reacting to the presence of ants walking nearby with an extremely rapid movement of the wings, even though there was no physical contact between them.
DOI: 10.25318/3710018601-eng
title: Work-integrated learning participation of postsecondary graduates, by province of study, level of study, field of study and sex, inactive

description: Statistics on the work-integrated learning (WIL) participation of postsecondary graduates, including whether or not the participation was mandatory or paid, are presented by the province of study, the level of study, the field of study and sex.
DOI: 10.25318/3710018601-fra
title: Participation à l'apprentissage intégré au travail des diplômés postsecondaires, selon la province d'études, le niveau d'études, le domaine d'études et le sexe, inactif

description: Les statistiques sur la participation à l'apprentissage intégré au travail (AIT) des diplômés postsecondaires, y compris si la participation était obligatoire ou rémunérée, sont présentées selon la province d'études, le niveau d'études, le domaine d'études et le sexe.
DOI: 10.5281/zenodo.10849394
title: m6A-dependent 7-dehydrocholesterol reductase facilitates bladder cancer metastasis via cAMP/PKA/FAK axis

description: Cholesterol homeostasis dysregulation appears in multiple tumors. However, the specific processes that cause abnormal cholesterol metabolism to affect the invasion and metastasis of bladder cancer (BC) are still unclear. In our investigation, we found a notable rise in the expression of 7-dehydrocholesterol reductase (DHCR7), a key enzyme involved in the synthesis of cholesterol, within BC tissues in comparison to normal tissues and correlated to the invasion and metastasis of BC. This elevated expression of DHCR7 in BC was attributed to the decreased degradation of mRNA mediated by YTHDF2. We discovered that DHCR7 plays a role in promoting bladder cancer invasion and metastasis by activating the cAMP-PKA-FAK pathway. Specifically, DHCR7 was found to increase the levels of cAMP by enhancing cholesterol content in lipid rafts, thereby facilitating the transduction of signaling pathways mediated by cAMP receptors. Additionally, DHCR7 was found to enhance the cAMP signaling pathway by reducing the concentration of 7-DHC and promoting the transcription of GIPR. Overall, our findings demonstrated that DHCR7 plays a crucial role in BC invasion and metastasis by modulating cholesterol synthesis and cAMP signaling pathways. Furthermore, AY9944, which acts as an inhibitor of DHCR7, shows promise as a viable therapeutic strategy for the suppression of invasion and metastasis in BC.
DOI: 10.5281/zenodo.10849395
title: m6A-dependent 7-dehydrocholesterol reductase facilitates bladder cancer metastasis via cAMP/PKA/FAK axis

description: Cholesterol homeostasis dysregulation appears in multiple tumors. However, the specific processes that cause abnormal cholesterol metabolism to affect the invasion and metastasis of bladder cancer (BC) are still unclear. In our investigation, we found a notable rise in the expression of 7-dehydrocholesterol reductase (DHCR7), a key enzyme involved in the synthesis of cholesterol, within BC tissues in comparison to normal tissues and correlated to the invasion and metastasis of BC. This elevated expression of DHCR7 in BC was attributed to the decreased degradation of mRNA mediated by YTHDF2. We discovered that DHCR7 plays a role in promoting bladder cancer invasion and metastasis by activating the cAMP-PKA-FAK pathway. Specifically, DHCR7 was found to increase the levels of cAMP by enhancing cholesterol content in lipid rafts, thereby facilitating the transduction of signaling pathways mediated by cAMP receptors. Additionally, DHCR7 was found to enhance the cAMP signaling pathway by reducing the concentration of 7-DHC and promoting the transcription of GIPR. Overall, our findings demonstrated that DHCR7 plays a crucial role in BC invasion and metastasis by modulating cholesterol synthesis and cAMP signaling pathways. Furthermore, AY9944, which acts as an inhibitor of DHCR7, shows promise as a viable therapeutic strategy for the suppression of invasion and metastasis in BC.
DOI: 10.5281/zenodo.10850264
title: NSRC-Search: Efficient searching for similar protein sequences of non-standard amino acid composition

description: This research was funded by the National Science Centre in Poland (grant number 2021/41/N/ST6/01919)
DOI: 10.5281/zenodo.10850265
title: NSRC-Search: Efficient searching for similar protein sequences of non-standard amino acid composition

description: This research was funded by the National Science Centre in Poland (grant number 2021/41/N/ST6/01919)
DOI: 10.25318/3710018701-eng
title: Work-integrated learning participation of postsecondary graduates, by province of residence at interview, level of study, field of study and sex, inactive

description: Statistics on postsecondary graduates participation in work-integrated learning (WIL), including whether or not the participation was mandatory or paid, are presented by the province of residence at interview, the level of study, the field of study and sex.
DOI: 10.25318/3710018701-fra
title: Participation à l'apprentissage intégré au travail des diplômés postsecondaires, selon la province de résidence à l'interview, le niveau d'études, le domaine d'études et le sexe, inactif

description: Les statistiques sur la participation à l'apprentissage intégré au travail (AIT) des diplômés postsecondaires, y compris si la participation était obligatoire ou rémunérée, sont présentées selon la province de résidence à l'interview, le niveau d'études, le domaine d'études et le sexe.
DOI: 10.6084/m9.figshare.25452511
title: <b>Estudo Qualitativo - Dados gerados pelo IRaMuTeQ</b>

description: A doença causada pelo vírus SARS-CoV-2, conhecida como COVID-19, foi declarada em março de 2020 pandemia de COVID-19 pela Organização Mundial da Saúde (OMS) e, até 11 de junho de 2023, foram confirmados no mundo mais de 767 milhões de casos e mais de 6,9 milhões de mortes. Dados apontam que cerca de 19% dos pacientes infectados necessitam de hospitalização e 5% necessitam de internação na UTI devido à maior gravidade clínica. Contudo, após o quadro agudo pelo coronavírus, observou-se a persistência de sintomas por semanas a meses, em diversos sistemas orgânicos, e efeitos psicológicos, que levam a perda de produtividade, a dificuldade de integração na sociedade e do retorno as atividades diárias e ao trabalho, como também, realizando mudanças nos fatores que influenciam as condições crônicas. Dessa forma, este estudo tem por objetivo conhecer a percepção de pacientes com doenças cardiometabólicas sobre as adaptações no seu estilo de vida após acometimento pela covid-19. Trata-se de um estudo exploratório com abordagem qualitativa, desenvolvido em Unidade Básica de Saúde, no munícipio do interior do estado do Ceará. Contou com a participação de nove pacientes com o diagnóstico de alguma das doenças cardiometabólicas e que tiveram teste laboratorial positivo para covid-19. A coleta ocorreu a partir de entrevistas semiestruturadas áudio gravadas e seu conteúdo foi transcrito e armazenados em Google Drive, posteriormente, foram processados no software IRAMUTEQ. A pesquisa obedeceu aos aspectos éticos da Resolução 466/12 do Conselho Nacional de Saúde (CNS), sendo submetida e aprovada pelo Comitê de Ética em Pesquisa da Universidade da Integração Internacional da Lusofonia Afro-Brasileira, com parecer de n° 4.429.720.