cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/teg9e-qap67
title: Agro C58 22C liq

description: Agrobacterium tumefaciens
description: <strong>Tilt Series Date:</strong> 2014-05-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> From Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown at 22C and induced with AS for one day in liquid</p>
<strong>Sample Preparation:</strong> Cells were not concentrated prior to freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-2/rawdata/</p>c58_22C_liq0002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-2/rawdata/c58_22C_liq0002.mrc"
        > <i class="download icon"></i> Download </a></p>

        et2014-05-27-2_full.rec,  Reconstruction (Pixel Size 0.0 nm), 769.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-2/3dimage_49475/et2014-05-27-2_full.rec"
        > <i class="download icon"></i> Download </a></p>

        et2014-05-27-2.tif,  2.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-2/file_11672/et2014-05-27-2.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.60707/zdm2-5714
title: HAADF STEM images of 'vesicle 1' in OREX-501044-104
DOI: 10.22002/5693w-qr680
title: Chromogranin A-GFP

description: CLEM of chromogranin A-GFP expressing INS-1E cells
description: <strong>Tilt Series Date:</strong> 2014-08-25</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Chromogranin A GFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Friedberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both side pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads added to grids before freezing.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-20/rawdata/</p>tomogram4.mrc,  Tilt Series (Pixel Size 0.96 nm), 861.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-20/rawdata/tomogram4.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomogram4.rec,  Reconstruction (Pixel Size 3.84 nm), 443.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-20/3dimage_45795/tomogram4.rec"
        > <i class="download icon"></i> Download </a></p>

        tomogram4.rec,  Reconstruction (Pixel Size 0.0 nm), 133.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-08-25-20/3dimage_55701/tomogram4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8zqmh-v9a93
title: Helicobacter

description: T4SS search
description: <strong>Tilt Series Date:</strong> 2008-02-28</p>
<strong>Data Taken By:</strong> Megan Dobro</p>
<strong>Species / Specimen:</strong> Helicobacter hepaticus</p>
<strong>Strain:</strong> ATCC 51449</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-70.0°, 65.0°), step: 1 degree increment, exp=300°, constant angular increment, dosage: 2417.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Janet Chow, Mazmanian Lab; prepared Helicobacter</p>
<strong>Purification / Growth Conditions / Treatment:</strong> mutant 242</p>
<strong>Sample Preparation:</strong> R 2/1 quantifoil grids, 10 nm gold, 100% humidity, box 940</p>
  mdj2008-02-28-7_slicer4584.jpg: Flagellar motor mdj2008-02-28-7_slicer4587.jpg: Chemoreceptors mdj2008-02-28-7_slicer4589.jpg: nested granule mdj2008-02-28-7_slicer4591.jpg: Intracellular? Vesicle
description: Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2008-02-28-7/3dimage_2923/</p>Hhepaticus_242_01_full.rec,  Reconstruction (Pixel Size 15.64 nm) See same "lines" between membranes as in WT, 157.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2008-02-28-7/3dimage_2923/Hhepaticus_242_01_full.rec"
        > <i class="download icon"></i> Download </a></p>

        Hhepaticus_242_01.tif,  8.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2008-02-28-7/file_1205/Hhepaticus_242_01.tif"
        > <i class="download icon"></i> Download </a></p>

        mdj2008-02-28-7_slicer16445.png,  REZ .  -andrew, 345.4 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/mdj2008-02-28-7_slicer16445.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vh95z-y6746
title: T. intermedia autotrophic 26 hrs

description: T. intermedia cell h4 grown in autotrophic conditions for 26 hours
description: <strong>Tilt Series Date:</strong> 2007-11-19</p>
<strong>Data Taken By:</strong> Cristina Iancu</p>
<strong>Species / Specimen:</strong> Thiomonas intermedia</p>
<strong>Strain:</strong> K12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -13.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Purification / Growth Conditions / Treatment:</strong> growth in autotrophic conditions for 26 hours</p>
  civ2007-11-19-7_slicer7183.jpg: s-layer .  peptidoglycan civ2007-11-19-7_slicer7186.jpg: filament civ2007-11-19-7_slicer7189.jpg: tube
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-19-7/rawdata/</p>Ti_a26h_h4_200e13um18K_19nov07.mrc,  Tilt Series (Pixel Size 1.259 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-19-7/rawdata/Ti_a26h_h4_200e13um18K_19nov07.mrc"
        > <i class="download icon"></i> Download </a></p>

        Ti_a26h_h4_200e13um18K_19nov07_bin2.rec,  Reconstruction (Pixel Size 0.0 nm), 109.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-19-7/3dimage_4104/Ti_a26h_h4_200e13um18K_19nov07_bin2.rec"
        > <i class="download icon"></i> Download </a></p>

        civ2007-11-19-7_slicer19165.png,  REZ .  This region appears to be rich in small vesicle like objects with extremely poor contrast.  It's not clear what is occupying this space. .  -andrew, 439.0 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/civ2007-11-19-7_slicer19165.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tcbwm-gzw93
title: Thiomicrospira crunogena

description: T. crunogena cell h6
description: <strong>Tilt Series Date:</strong> 2007-11-21</p>
<strong>Data Taken By:</strong> Cristina Iancu</p>
<strong>Species / Specimen:</strong> Hydrogenovibrio crunogenus</p>
<strong>Strain:</strong> XCL-2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -13.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
  civ2007-11-21-1_slicer4490.jpg: Extracellular vesicle civ2007-11-21-1_slicer4492.jpg: Carboxysome civ2007-11-21-1_slicer4494.jpg: Unknown filament civ2007-11-21-1_slicer4497.jpg: Unknown tube .  [2014-07-23 14:24:28] bullseye civ2007-11-21-1_slicer4499.jpg: Unknown tube civ2007-11-21-1_slicer4503.jpg: Unknown tube civ2007-11-21-1_slicer4505.jpg: Unknown tube civ2007-11-21-1_slicer4507.jpg: Intracellular vesicle civ2007-11-21-1_slicer4508.jpg: Flagellar motor civ2007-11-21-1_slicer4510.jpg: Flagellar motor
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-21-1/rawdata/</p>Tc_Tch6_200e13um18K_21nov07.mrc,  Tilt Series (Pixel Size 1.259 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-21-1/rawdata/Tc_Tch6_200e13um18K_21nov07.mrc"
        > <i class="download icon"></i> Download </a></p>

        Tc_Tch6_200e13um18K_21nov07_crop_bin2.rec,  Reconstruction (Pixel Size 0.0 nm), 91.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-21-1/3dimage_3895/Tc_Tch6_200e13um18K_21nov07_crop_bin2.rec"
        > <i class="download icon"></i> Download </a></p>

        Tc_Tch6_200e13um18K_21nov07_crop_bin2_s107.tif,  1.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/civ2007-11-21-1/file_1457/Tc_Tch6_200e13um18K_21nov07_crop_bin2_s107.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/az0fk-tpz40
title: MEFs (Rho-) Chan Lab

description: Mef cell line (Rho-) Chan Lab
description: <strong>Tilt Series Date:</strong> 2012-05-29</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>
<strong>Strain:</strong> Primary cells</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-8/3dimage_69375/</p>12may29_C135_1_MEFnomitoDNA_AT1BT30009_SIRT.rec,  Reconstruction (Pixel Size 2.0 nm), 2.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-8/3dimage_69375/12may29_C135_1_MEFnomitoDNA_AT1BT30009_SIRT.rec"
        > <i class="download icon"></i> Download </a></p>

        12may29_C135_1_MEFnomitoDNA_AT1BT30009_full.rec,  Reconstruction (Pixel Size 2.0 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-8/3dimage_69374/12may29_C135_1_MEFnomitoDNA_AT1BT30009_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12may29_C135_1_MEFnomitoDNA_AT1BT30009.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-8/rawdata/12may29_C135_1_MEFnomitoDNA_AT1BT30009.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gmgp5-bq640
title: Alongum

description: Alongum_periplasmiclayersREZ (ribosome-exclusion-zones)
description: <strong>Tilt Series Date:</strong> 2012-05-20</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Acetonema longum</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-30.0°, 30.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> 0</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> uploadraptor</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> WesleyChen helped remove layers</p>
<strong>Purification / Growth Conditions / Treatment:</strong> sporulation media</p>
<strong>Sample Preparation:</strong> quantifoil grid R2/2, 20nm gold, 100% humidity</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-14/rawdata/</p>AlongLys0016.mrc,  Tilt Series (Pixel Size 0.95 nm), 677.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-14/rawdata/AlongLys0016.mrc"
        > <i class="download icon"></i> Download </a></p>

        AlongLys0016_full.rec,  Reconstruction (Pixel Size 1.95 nm), 309.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2012-05-20-14/3dimage_56748/AlongLys0016_full.rec"
        > <i class="download icon"></i> Download </a></p>

        etl2012-05-20-14_slicer14135.png,  622.5 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/etl2012-05-20-14_slicer14135.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6j45j-8c561
title: ori5 CH4

description: Raw data files of ori5 CH4
description: <strong>Tilt Series Date:</strong> 2014-02-13</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Methylomicrobium alcaliphilum</p>
<strong>Strain:</strong> 20Z-ori5</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor, IMOD</p>
<strong>Collaborators and Roles:</strong> Marina Kalyuzhnaya @ Univ. Washington provided the culture</p>
<strong>Purification / Growth Conditions / Treatment:</strong> P-medium, 3% NaCl, supplemented with methane (20% headspace)</p>
  scy2014-02-13-8_slicer3953.jpg: intra-cytoplasmic membrane arrays;
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-8/rawdata/</p>atlas50001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-8/rawdata/atlas50001.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas50001_part121_34.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-8/3dimage_38883/atlas50001_part121_34.rec"
        > <i class="download icon"></i> Download </a></p>

        at501b4.rec,  Reconstruction (Pixel Size 1.9 nm), 298.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-8/3dimage_39670/at501b4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.5281/zenodo.16945399
title: Assessing Eastern Canada's Intraplate Seismicity Through Microplate Tectonics and Strain Rate Analyses: Data and Model Repository

description: This is the data and model repository for the Earth Science Reviews manuscript entitled "Assessing Eastern Canada’s Intraplate Seismicity Through Microplate Tectonics and Strain Rate Analyses".
DOI: 10.22002/y3dj2-qga17
title: COS7 VPS4DN+CHMP4A

description: Cos7 cells co-transfected with VPS4DN and CHMP4A. Sample #C104
description: <strong>Tilt Series Date:</strong> 2012-02-04</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN and CHMP4A constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-14/3dimage_68707/</p>12feb04_ATBT1_0015_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-14/3dimage_68707/12feb04_ATBT1_0015_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0015.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-14/rawdata/12feb04_ATBT1_0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        12feb04_ATBT1_0015_full.tiff,  file associated to 3D Image #68707, 1.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-04-14/file_15959/12feb04_ATBT1_0015_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.17909/h2xq-8534
title: Optical narrow band
DOI: 10.22002/2sj98-rze85
title: COS7 pNLGFP INF-alpha

description: Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110
description: <strong>Tilt Series Date:</strong> 2012-01-20</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-26/3dimage_69049/</p>12jan20_C110_2_pNL_INFalpha_AT1BT1_0005_full.rec,  Reconstruction (Pixel Size 2.0 nm), 924.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-26/3dimage_69049/12jan20_C110_2_pNL_INFalpha_AT1BT1_0005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_2_pNL_INFalpha_AT1BT1_0005.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-20-26/rawdata/12jan20_C110_2_pNL_INFalpha_AT1BT1_0005.st"
        > <i class="download icon"></i> Download </a></p>

        12jan20_C110_2_pNL_INFalpha_AT1BT1_0005_full.tiff,  file associated to 3D Image #69049, 1.0 MB  
        
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DOI: 10.22002/cy4s9-fat33
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-21</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-21-10/rawdata/</p>tomography4.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
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        tomography4.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
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        tomography4_full.rec,  Reconstruction (Pixel Size 0.0 nm), 444.5 MB  
        
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