DOI: 10.5061/dryad.bnzs7h4h8
title: Effective dispersal patterns in prairie plant species across human-modified landscapes

description: Effective dispersal among plant populations is dependent on vector
 behavior, landscape features, and availability of adequate habitats. To
 capture landscape feature effects on dispersal, studies must be conducted
 at scales reflecting single-generation dispersal events (meso-scale). Many
 studies are conducted at large-scales where genetic differentiation is due
 to dispersal occurring over multiple generations, making it difficult to
 interpret the effects of specific landscape features on vector behavior.
 Genetic structure at the meso-scale may be determined by ecological and
 evolutionary processes, such as the consequences of vector behavior on
 patterns of gene flow. We used chloroplast haplotypes and nuclear genome
 SNP surveys to identify landscape features influencing seed and pollen
 dispersal at a meso-scale within the Rogue River Valley in southern
 Oregon. We evaluated biotic and abiotic vector behavior by contrasting two
 annual species with differing dispersal mechanisms; Achyrachaena mollis
 (Asteraceae) is a self-pollinating and anemochoric species, and Plectritis
 congesta (Caprifoliaceae) is biotically pollinated with barochoric seeds.
 Using landscape genetics methods, we identified features of the study
 region that conduct or restrict dispersal. We found chloroplast haplotypes
 were indicative of historic patterns of gene flow prior to human
 modification of landscapes. Seed dispersal of A. mollis was best supported
 by models of isolation-by-distance, while seed-driven gene flow of P.
 congesta was determined by the distribution of preserved natural spaces
 and quality habitat. Whole-genome genetic structure was driven by both
 pollen and seed dispersal, and both species responded to contemporary
 landscape changes, such as urban and agricultural conversion, and habitat
 availability.
description: # Effective dispersal patterns in prairie plant species across
 human-modified landscapes
 [https://doi.org/10.5061/dryad.bnzs7h4h8](https://doi.org/10.5061/dryad.bnzs7h4h8) ## Description of the data and file structure **Species_sampling_coordinates.csv**: CSV containing the species, population alpha-numeric code, sequencing type (chloroplast haplotypes, genotyping-by-sequencing, or both) and latitude-longitude coordinates. **Amollis_cp_haplotype_population_frequencies.csv**: CSV containing the chloroplast haplotype frequencies by population for *Achyrachaena mollis*, as determined by the CallHap program. Each row is a population; each column is an identified haplotype. Values represent the number of individuals in the population with the haplotype. **Pcongesta_cp_haplotype_population_frequencies.csv**: CSV containing the chloroplast haplotype frequencies by population for *Plectritis congesta*, as determined by the CallHap program. Each row is a population; each column is an identified haplotype. Values represent the number of individuals in the population with the haplotype. **Amollis_GBS_loci.str**: STR file containing the loci retained for *A. mollis* after calling by GBS-SNP-CROP and downstream filtering by TASSEL. **Pcongesta_GBS_loci.str**: STR file containing the loci retained for *P. congesta* after calling by GBS-SNP-CROP and downstream filtering by TASSEL. ## Code/Software R and shell scripts used during analysis can be found at [https://github.com/cruzan-lab/landscape-genetics. ](https://github.com/cruzan-lab/landscape-genetics.)