DOI: 10.5061/dryad.qrfj6q5mb
title: Data for: Wetland productivity determines trade-off between biodiversity and greenhouse gas production

description: The data consists of wetland nutrient, chlorophyll-a, methane and nitrous
 oxide concentrations, as well as emergence data of chironomid midges, and
 water temperatures. The data collection took place in 2021 in May, June,
 July, August and October, with simultaneous collection of chironomid
 emergence and water chemistry. We also calculated stoichiometric nitrogen
 (N) to phosphorus (P) ratios (N:P), as well as methane and nitrous oxide
 fluxes. The sampled chironomids were identified to genus level for all
 sampled months, apart from October, from which Shannon-Wiener taxonomic
 diversity was calculated. Additionally, genera were categorized into
 feeding groups based on literature and morphology from which functional
 feeding group Shannon-Wiener functional diversity was
 calculated.  
description: The dataset was collected in wetlands and ponds in eastern Sweden
 in 2021. Insects were collected at five periods (May, June, July, August
 and October) in 2021, using floating emergence traps containing soapy
 water for 72h, and later preserved in 70% ethanol for conservation.
 Collected insects were counted and identified to genus level, and divided
 into functional feeding groups. Water nutrient samples were collected when
 insect traps were set out and at the same sampling point and were kept
 refrigerated until chemical analyses were performed at the Geochemical
 Laboratory at the Swedish University of Agricultural Sciences in Uppsala,
 Sweden. Stoichiometric molar nitrogen:phosphorus ratios was calculated
 from nutrient concentrations. Greenhouse gas (GHG) data was collected
 using the headspace method: 30 ml of water from 5 cm depth into a 60 ml
 syringe, and then 30 ml of ambient air one m above the wetland. the
 syringe was shaken vigorously for 60 seconds and 15 ml of the headspace
 gas from the syringe was transferred to a 21 ml Exetainer vial for
 analysis. Methane and nitrous oxide were then analyzed using a Clarus 500
 gas chromatograph with a flame ionisation detector and an electron capture
 detector. GHG concentrations were converted from ppm to dissolved
 concentrations using the solubility functions from Wiesenburg and Guinasso
 (1979) and Weiss and Price (1980), accounting for water temperature and
 atmospheric pressure at time of sampling, water:air column in the syringe,
 and ambient air concentration. Chlorophyll-a concentrations were measured
 as a proxy for aquatic primary production using a FloroSense chlorophyll
 probe. 
description: # Data for: Wetland productivity determines trade-off between biodiversity
 support and greenhouse gas production The dataset was collected in
 wetlands and ponds in eastern Sweden in 2021. Insects were collected at
 five periods (May, June, July, August and October) in 2021, using floating
 emergence traps containing soapy water during 72h, and later preserved in
 70% ethanol for conservation. Collected insects were counted and
 identified to genus level, and divided into functional feeding groups.
 Water nutrient samples were collected when insect traps were set out and
 at the same sampling point and were kept refrigerated until chemical
 analyses were performed at the Geochemical Laboratory at the Swedish
 University of Agricultural Sciences in Uppsala, Sweden. Stoichiometric
 molar nitrogen:phosphorus ratios were calculated from nutrient
 concentrations. Greenhouse gas (GHG) data was collected using the
 headspace method: 30 ml of water from 5 cm depth into a 60 ml syringe, and
 then 30 ml of ambient air one m above the wetland. the syringe was shaken
 vigorously for 60 seconds and 15 ml of the headspace gas from the syringe
 was transferred to a 21 ml Exetainer vial for analysis. Methane and
 nitrous oxide were then analyzed using a Clarus 500 gas chromatograph with
 a flame ionisation detector and an electron capture detector. GHG
 concentrations were converted from ppm to dissolved concentrations using
 the solubility functions from Wiesenburg and Guinasso (1979) and Weiss and
 Price (1980), accounting for water temperature and atmospheric pressure at
 time of sampling, water:air column in the syringe, and ambient air
 concentration. Chlorophyll-a concentrations were measured as a proxy for
 aquatic primary production using a FloroSense chlorophyll probe. ##
 Description of the data and file structure The dataset consists of wetland
 productivity data and location information, including nitrogen and
 phosphorus concentrations, water temperature, chlorophyll-a
 concentrations, stoichiometric N:P ratios, methane- and nitrous oxide
 concentrations and fluxes, and Chironomidae hatch rates, genus, and
 functional group across all sampling periods and locations. The data
 consists of four tab-spaced text files: 1. nutrient\_data\_compl.txt 2.
 nutrient\_data\_compl\_avg.txt 3. chiro\_matrix.txt 4.
 funct\_food\_pref\_mat.txt nutrient\_data\_compl.txt contains: * sample:
 Sample ID given, used for uniting datasets. * loc: Designated name of the
 location. * chiro: Total abundance of chironomid midges in the sample [n].
 * chlor: Measured chlorophyll-a concentration at sample collection point
 [µg/L]. * ntot: Total measured concentration of nitrogen at sample
 collection point [µg/L]. * ptot: Total measured concentration of
 phosphorus at sample collection point [µg/L]. * ch4: Measured
 concentration of dissolved methane at sample collection point [µg/L]. *
 n2o: Measured concentration of dissolved nitrous oxide at sample
 collection point [µg/L]. * np: Ratio between ntot and ptot at sample
 collection point [N:P ratio]. * wtemp: Water temperature measured at
 sample collection point [°C]. * n2o\_flux: Calculated nitrous oxide flux
 at sample collection point [µg/m<sup>2</sup>/h]. * ch4\_flux:
 Calculated methane flux at sample collection point
 [mg/m<sup>2</sup>/h]. * month: Month of collection.
 nutrient\_data\_compl\_avg.txt contains: * loc: Designated name of the
 location. * tot\_chiro: Cumulative abundance of chironomids collected
 across all months [n]. * h\_tax\_tot: Shannon-Wiener genus diversity index
 for all samples and genera across all months except October [H]. *
 h\_food\_tot: Shannon-Wiener feeding group diversity index for all samples
 and genera across all months except October [H]. * avg\_chlor: Average
 measured chlorophyll-a concentration at sample collection point across all
 months [µg/L]. * avg\_ntot: Average measured concentration of nitrogen at
 sample collection point across all months [µg/L]. * avg\_ptot: Average
 measured concentration of phosphorus at sample collection point across all
 months [µg/L]. * avg\_ch4: Average measured concentration of dissolved
 methane at sample collection point across all months [µg/L]. * avg\_n2o:
 Average measured concentration of dissolved nitrous oxide at sample
 collection point across all months [µg/L]. * avg\_np: Average ratio
 between ntot and ptot at sample collection point across all months [N:P
 ratio]. * avg\_n2o\_flux: Average calculated nitrous oxide flux at sample
 collection point across all months [µg/m<sup>2</sup>/h]. *
 avg\_ch4\_flux: Average calculated methane flux at sample collection point
 across all months [mg/m<sup>2</sup>/h]. chiro\_matrix.txt
 contains: * sample: Sample ID given, used for uniting datasets. *
 "columns 2 through 44": Abundances of genera of chironomids in
 the sample [n]. funct\_food\_pref\_mat.txt contains: * sample: Sample ID
 given, used for uniting datasets. * Alg: The abundance of algae eaters
 chironomid functional group in the sample [n]. * Ani: The abundance of
 animal eaters chironomid functional group in the sample [n]. * Det: The
 abundance of detritivorous chironomid functional group in the sample [n].
 * Omn: The abundance of omnivorous chironomid functional group in the
 sample [n]. * Veg: The abundance of vegetation eaters chironomid
 functional group in the sample [n]. ## Code/Software All analyses were run
 in R (ver. 4.2.1), with libraries and analysis code specified in the
 accompanying script file.