cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/xaahw-3eb50
title: Hpylori_dFliF_FISE

description: These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out  .  to examine its relation to the hat-like complex.
description: <strong>Tilt Series Date:</strong> 2020-10-08</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -8.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> The cells were prepared and sent by Carrie Schaffer from the university of Kentucky. The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C. No growth was seen . on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100%  humidity. Cu grids. 10 nm . gold</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-330/rawdata/</p>Hpylori_DFliF_FISE_335.mrc,  Tilt Series (Pixel Size 0.452 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-330/rawdata/Hpylori_DFliF_FISE_335.mrc"
        > <i class="download icon"></i> Download </a></p>

        Hpylori_DFliF_FISE_335_full.rec,  Reconstruction (Pixel Size 1.808 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-10-08-330/3dimage_114137/Hpylori_DFliF_FISE_335_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a6tby-cx564
title: P_aeruginosa_PA14_DfliF_Krios

description: Raw data files of P_aeruginosa_PA14_DfliF_Krios
description: <strong>Tilt Series Date:</strong> 2018-09-29</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (50.0°, -50.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> I (mohammed) obtained the cells from Shannon Park (Dianne Newman lab). The cells are available in their library of Pseudomonas aeruginosa PA14.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated one colony from an LB agar plate and grew the cells overnight in LB with 100 ug/ml of Gentamycin. Then I concentrated the cells three times and mixed them with gold nanoparticles and added 3 ul on to thick carbon R2 grids and blotted them and plunge froze them (force either 6 or 3, time either 3 or 2 seconds respectively, drain time 1 second).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-43/rawdata/</p>Pseudomonas_DfliF41.mrc,  Tilt Series (Pixel Size 0.53 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-43/rawdata/Pseudomonas_DfliF41.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudomonas_DfliF41_full.rec,  Reconstruction (Pixel Size 2.12 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-43/3dimage_97228/Pseudomonas_DfliF41_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/z6bn9-e0a52
title: JV9082_K3_janelia

description: JV9082:DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2019-12-04</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Å²,  defocus: -3.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-04-32/rawdata/</p>9082_Box1_No4_S66.mrc,  Tilt Series (Pixel Size 0.261 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-04-32/rawdata/9082_Box1_No4_S66.mrc"
        > <i class="download icon"></i> Download </a></p>

        9082_Box1_No4_S66_full.rec,  Reconstruction (Pixel Size 1.044 nm), 737.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-04-32/3dimage_113003/9082_Box1_No4_S66_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/g9f83-8mf74
title: P_aeruginosa_PA14_DfliF_Krios

description: Raw data files of P_aeruginosa_PA14_DfliF_Krios
description: <strong>Tilt Series Date:</strong> 2018-09-29</p>
<strong>Data Taken By:</strong> Mohammed Kaplan</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (50.0°, -50.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -9.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Collaborators and Roles:</strong> I (mohammed) obtained the cells from Shannon Park (Dianne Newman lab). The cells are available in their library of Pseudomonas aeruginosa PA14.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> I inoculated one colony from an LB agar plate and grew the cells overnight in LB with 100 ug/ml of Gentamycin. Then I concentrated the cells three times and mixed them with gold nanoparticles and added 3 ul on to thick carbon R2 grids and blotted them and plunge froze them (force either 6 or 3, time either 3 or 2 seconds respectively, drain time 1 second).</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-55/rawdata/</p>Pseudomonas_DfliF52.mrc,  Tilt Series (Pixel Size 0.53 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-55/rawdata/Pseudomonas_DfliF52.mrc"
        > <i class="download icon"></i> Download </a></p>

        Pseudomonas_DfliF52_full.rec,  Reconstruction (Pixel Size 2.12 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2018-09-29-55/3dimage_97247/Pseudomonas_DfliF52_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.r3g4sd
title: Occurrence Download

description: A dataset containing 2458 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is Specimen",
 "OccurrenceStatus is Present",
 "PublishingOrg is one of (ae447c50-b8a8-11d8-92a4-b8a03c50a862, 90fd6680-349f-11d8-aa2d-b8a03c50a862, 396d5f30-dea9-11db-8ab4-b8a03c50a862, 061b4f20-f241-11da-a328-b8a03c50a862, 2cd829bb-b713-433d-99cf-64bef11e5b3e, bc092ff0-02e4-11dc-991f-b8a03c50a862)",
 "TaxonKey is Mangifera indica"
 ]
}

 The dataset includes 2458 records from 8 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007936-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/cfprg-h7z31
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-55/rawdata/</p>JV9082_201808290028.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-55/rawdata/JV9082_201808290028.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_201808290028_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-55/3dimage_96436/JV9082_201808290028_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ccb0c-se155
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-281/rawdata/</p>JV9082_20180901a0055.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-281/rawdata/JV9082_20180901a0055.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0055_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-281/3dimage_96985/JV9082_20180901a0055_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/db1xf-1m075
title: D. flos-aquae Gas Vesicles

description: Gas vesicles purified from D. flos-aquae, WT
description: <strong>Tilt Series Date:</strong> 2020-11-04</p>
<strong>Data Taken By:</strong> Przemek Dutka</p>
<strong>Species / Specimen:</strong> Dolichospermum flos-aquae</p>
<strong>Strain:</strong> CCAP 1403/13F</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Å²,  defocus: 2.5 μm,  magnification: 64000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> </p>
<strong>Collaborators and Roles:</strong> purified by Dina Malounda the Shapiro lab; data collected by Przemek . Dutka and Songye Chen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Lakshmanan, A., Lu, G., Farhadi, A. et al. Preparation of biogenic gas . vesicle nanostructures for use as contrast agents for ultrasound and . MRI. Nat Protoc 12, 2050–2080 (2017). . https://doi.org/10.1038/nprot.2017.081. Sample in 10mM Hepes, pH7.4; OD 5.0</p>
<strong>Sample Preparation:</strong> Quantifoil C2/2 200mesh extra thick carbon, 10nm BSA-gold, 100% . humidity, Blot time 4, Blot force 3, temp 4C</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-12/rawdata/</p>AnaGVOD5_8_movies.mrc,  Tilt Series (Pixel Size 0.14 nm), 1.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-12/rawdata/AnaGVOD5_8_movies.mrc"
        > <i class="download icon"></i> Download </a></p>

        AnaGVOD5_8.mrc.mdoc,  26.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-12/rawdata/AnaGVOD5_8.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ev6eb-wt060
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-241/rawdata/</p>JV9082_20180901a0015.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-241/rawdata/JV9082_20180901a0015.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_20180901a0015_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-241/3dimage_96890/JV9082_20180901a0015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.d22jv7
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Copadichromis diplostigma, Copadichromis geertsi, Copadichromis ilesi, Copadichromis insularis, Copadichromis jacksoni, Copadichromis likomae, Copadichromis mbenjii, Copadichromis melas, Copadichromis mloto, Copadichromis nkatae, Copadichromis parvus, Copadichromis pleurostigma, Copadichromis pleurostigmoides, Copadichromis quadrimaculatus, Copadichromis trewavasae, Copadichromis trimaculatus, Copadichromis verduyni, Copadichromis virginalis, Coptodon bakossiorum, Coptodon bemini, Coptodon bythobates, Coptodon cameronensis, Coptodon camerunensis, Coptodon coffea, Coptodon congica, Coptodon dageti, Coptodon deckerti, Coptodon discolor, Coptodon ejagham, Coptodon flava, Coptodon fusiforme, Coptodon guineensis, Coptodon gutturosa, Coptodon imbriferna, Coptodon ismailiaensis, Coptodon kottae, Coptodon louka, Coptodon margaritacea, Coptodon nigrans, Coptodon nyongana, Coptodon rendalli, Coptodon rheophila, Coptodon snyderae, Coptodon spongotroktis, Coptodon tholloni, Coptodon thysi, Coptodon walteri, Coptodon zillii, Coptostomabarbus bellcrossi, Coptostomabarbus wittei, Corematodus shiranus, Corematodus taeniatus, Coryogalops william, Cotylopus acutipinnis, Cotylopus rubripinnis, Crenimugil buchanani, Crenimugil crenilabis, Crenimugil seheli, Cromeria nilotica, Cromeria occidentalis, Ctenochromis horei, Ctenochromis luluae, Ctenochromis oligacanthus, Ctenochromis polli, Ctenogobius lepturus, Ctenopharyngodon idella, Ctenopharynx intermedius, Ctenopharynx nitidus, Ctenopharynx pictus, Ctenopoma acutirostre, Ctenopoma ashbysmithi, Ctenopoma kingsleyae, Ctenopoma machadoi, Ctenopoma multispine, Ctenopoma maculatum, Ctenopoma muriei, Ctenopoma nebulosum, Ctenopoma nigropannosum, Ctenopoma ocellatum, Ctenopoma petherici, Ctenopoma weeksii, Cunningtonia longiventralis, Cyathochromis obliquidens, Cyathopharynx furcifer, Cyclopharynx fwae, Cyclopharynx schwetzi, Cynotilapia afra, Cynotilapia aurifrons, Cynotilapia axelrodi, Cynotilapia chilundu, Cynotilapia pulpican, Cynotilapia zebroides, Microchromis zebroides, Cyphomyrus discorhynchus, Cyphomyrus macrops, Hippopotamyrus macrops, Cyphomyrus psittacus, Hippopotamyrus psittacus, Cyphomyrus wilverthi, Hippopotamyrus wilverthi)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((-16.8996 20.2064,-16.9237 20.0599,-16.8618 20.0599,-16.8618 22.7792,-17.2424 22.4474,-17.4477 21.9344,-17.5933 20.7331,-17.3825 20.3945,-16.8996 20.2064))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007939-250827131500795/datasets/export for details.
DOI: 10.22002/hc41m-shr41
title: JV9082_Janelia42k

description: DotF-sfGFP fusion
description: <strong>Tilt Series Date:</strong> 2018-08-29</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 42000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>
<strong>Sample Preparation:</strong> 6 grids were frozen for the JV9082 strain (2x concentrated culture); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-95/rawdata/</p>JV9082_201808290094.mrc,  Tilt Series (Pixel Size 0.26 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-95/rawdata/JV9082_201808290094.mrc"
        > <i class="download icon"></i> Download </a></p>

        JV9082_201808290094_full.rec,  Reconstruction (Pixel Size 1.04 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2018-08-29-95/3dimage_96540/JV9082_201808290094_full.rec"
        > <i class="download icon"></i> Download </a></p>