cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/1nmzd-mkv58
title: CheA-GE8-L2

description: silver binding peptide in linker 2 on CheAData collected on TITAN HHMI
description: <strong>Tilt Series Date:</strong> 2012-10-29</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> RP 437</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: °, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> FEI Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Carly Carter, Dan Feldheim</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase in TB, induced with 14 mM IPTG for 3 hours, lysed with penicillin for 1 hour, add DNAse buffer, DNAse for 20 min, add AgNO3 (0.014g AgNO3 in 1ml HEPES pH 7.2) and 50µl to 1 ml, incubated 20 min, spin down at 6krpm</p>
<strong>Sample Preparation:</strong> plunge freeze on Quantifoil R2/2 in liquid ethane/propane mix</p>
description: Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-12/rawdata/</p>CheA_AG-1_16_orig.st,  Tilt Series (Pixel Size 0.0 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-12/rawdata/CheA_AG-1_16_orig.st"
        > <i class="download icon"></i> Download </a></p>

        CheA_AG-1_16.rec,  Reconstruction (Pixel Size 0.0 nm), 2.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-10-29-12/3dimage_23706/CheA_AG-1_16.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2dwka-tdh37
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-51/rawdata/</p>20141022_YWC121_40021.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-51/rawdata/20141022_YWC121_40021.mrc"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_40021_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-51/3dimage_47493/20141022_YWC121_40021_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/jp244-05227
title: Hylemonella gracilis

description: Raw data files of Hylemonella gracilis
description: <strong>Tilt Series Date:</strong> 2012-09-23</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Hylemonella gracilis</p>
<strong>Strain:</strong> ATCC 19624</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 112.0 eV/Å²,  defocus: -20.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> RAPTOR</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix</p>
  ycw2012-09-23-49_slicer5041.jpg: REZ
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-49/rawdata/</p>20120923_Hylemonella_30012.mrc,  Tilt Series (Pixel Size 0.49 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-49/rawdata/20120923_Hylemonella_30012.mrc"
        > <i class="download icon"></i> Download </a></p>

        20120923_Hylemonella_30012_full.rec,  Reconstruction (Pixel Size 1.96 nm), 441.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-09-23-49/3dimage_20949/20120923_Hylemonella_30012_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/r20f6-r3838
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-07-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-58/3dimage_11543/</p>RCC745_MGtovis_199.rec,  Reconstruction (Pixel Size 1.26 nm), 180.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-58/3dimage_11543/RCC745_MGtovis_199.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_199.mrc,  Tilt Series (Pixel Size 1.26 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-58/rawdata/RCC745_MGtovis_199.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.72wmq2
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Cyphotilapia frontosa, Cyprichromis leptosoma, Cyprichromis microlepidotus, Cyrtocara moorii, Dagetichthys commersonnii, Synaptura commersonnii, Dagetichthys lakdoensis, Dalophis boulengeri, Dalophis cephalopeltis, Danakilia franchettii, Datnia elongata, Mesopristes elongatus, Denticeps clupeoides, Dermatopsoides kasougae, Dicentrarchus punctatus, Dicologlossa cuneata, Dimidiochromis compressiceps, Dimidiochromis dimidiatus, Dimidiochromis kiwinge, Dimidiochromis strigatus, Dinotopterus cunningtoni, Diplotaxodon aeneus, Diplotaxodon altus, Diplotaxodon argenteus, Diplotaxodon ecclesi, Diplotaxodon greenwoodi, Diplotaxodon limnothrissa, Diplotaxodon longimaxilla, Diplotaxodon macrops, Distichodus affinis, Distichodus altus, Distichodus antonii, Distichodus atroventralis, Distichodus brevipinnis, Distichodus decemmaculatus, Distichodus engycephalus, Distichodus fasciolatus, Distichodus hypostomatus, Distichodus kolleri, Distichodus langi, Distichodus lusosso, Distichodus maculatus, Distichodus mbiniensis, Distichodus microps, Distichodus mossambicus, Distichodus nefasch, Distichodus noboli, Distichodus notospilus, Distichodus petersii, Distichodus rostratus, Distichodus rufigiensis, Distichodus schenga, Distichodus sexfasciatus, Divandu albimarginatus, Docimodus evelynae, Docimodus johnstoni, Dolichallabes microphthalmus, Dolichamphilius brieni, Dolichamphilius longiceps, Dormitator lebretonis, Doumea angolensis, Doumea chappuisi, Doumea gracila, Doumea sanaga, Doumea thysi, Doumea typica, Drombus bontii, Gobius bontii, Dundocharax bidentatus, Ebomegobius goodi, Eclectochromis ornatus, Ectodus descampsii, Eleotris acanthopoma, Eleotris daganensis, Eleotris fusca, Eleotris mauritiana, Eleotris pellegrini, Eleotris senegalensis, Eleotris vittata, Eleotris vomerodentata, Ellochelon vaigiensis, Elops lacerta, Elops senegalensis, Engraulicypris bredoi, Engraulicypris brevianalis, Engraulicypris gariepinus, Engraulicypris moeruensis, Engraulicypris ngalala, Engraulicypris sardella, Engraulicypris spinifer, Enneacampus ansorgii, Enneacampus kaupi, Enteromius ablabes, Enteromius aboinensis, Enteromius afrohamiltoni, Enteromius afrovernayi, Enteromius aliciae, Enteromius aloyi, Enteromius amanpoae, Enteromius amatolicus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((18.2494 -34.8049,19.1382 -35.0665,19.1382 -33.9401,17.8437 -33.9401,17.8729 -34.3757,18.2494 -34.8049))"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0007889-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/hkq2d-7mw33
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-30/3dimage_11671/</p>RCC745_MGtovis_343.rec,  Reconstruction (Pixel Size 1.26 nm), 222.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-30/3dimage_11671/RCC745_MGtovis_343.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_343.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-30/rawdata/RCC745_MGtovis_343.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ccwma-0nj09
title: O. tauri Propyzamide release

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2010-02-12</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-142/rawdata/</p>RCC745_RELtovis_154.mrc,  Tilt Series (Pixel Size 1.26 nm), 562.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-142/rawdata/RCC745_RELtovis_154.mrc"
        > <i class="download icon"></i> Download </a></p>

        RCC745_RELtovis_154.rec,  Reconstruction (Pixel Size 1.26 nm), 224.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-02-12-142/3dimage_12625/RCC745_RELtovis_154.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/qmtet-nv011
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-10-28</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-8/3dimage_11627/</p>RCC745_MGtovis_320.rec,  Reconstruction (Pixel Size 1.26 nm), 272.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-8/3dimage_11627/RCC745_MGtovis_320.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_320.mrc,  Tilt Series (Pixel Size 1.26 nm), 755.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-10-28-8/rawdata/RCC745_MGtovis_320.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/dgrvy-tg487
title: O. tauri MG132 arrest

description: Ostreococcus tauri cryosections
description: <strong>Tilt Series Date:</strong> 2009-07-23</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Ostreococcus tauri</p>
<strong>Strain:</strong> RCC745</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132</p>
<strong>Sample Preparation:</strong> Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF </p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-75/3dimage_11577/</p>RCC745_MGtovis_247.rec,  Reconstruction (Pixel Size 1.26 nm), 185.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-75/3dimage_11577/RCC745_MGtovis_247.rec"
        > <i class="download icon"></i> Download </a></p>

        RCC745_MGtovis_247.mrc,  Tilt Series (Pixel Size 1.26 nm), 536.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2009-07-23-75/rawdata/RCC745_MGtovis_247.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xhy27-1q725
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC101
description: <strong>Tilt Series Date:</strong> 2014-05-13</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-13-15/rawdata/</p>20140513_YWC101_20008.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-13-15/rawdata/20140513_YWC101_20008.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140513_YWC101_20008_part131_18.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-05-13-15/3dimage_42461/20140513_YWC101_20008_part131_18.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/2a73b-vny66
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC121
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 200 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-39/rawdata/</p>20141022_YWC121_40007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-39/rawdata/20141022_YWC121_40007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20141022_YWC121_40007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-10-22-39/3dimage_47440/20141022_YWC121_40007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/0z31y-rbz14
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-03-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-07-10/rawdata/</p>20140306_YWC092_10005.mrc,  Tilt Series (Pixel Size 4.2 nm), 3.6 GB  
        
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        20140306_YWC092_10005_full.rec,  Reconstruction (Pixel Size 16.8 nm), 688.9 MB  
        
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