cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.6273719
title: Molecular identification and mapping of a novel stripe rust resistance gene in wheat resistance line CH5389

description: ABSTRACT: Stripe rust, caused by Puccinia striiformis is one of the most destructive diseases of wheat worldwide. CH5389 is a wheat-Thinopyrum intermedium derived line conferring stripe rust resistance. Genetic analyses of seedlings of F2 populations and F2:3 families developed by crossing CH5389 and susceptible common wheat revealed that stripe rust resistance in CH5389 was controlled by a single dominant gene that was designated YrCH5389. Eight SSR and EST-PCR polymorphic markers on chromosome 3AL were identified in F2 population of CH5389/Taichung29. The YrCH5389 was flanked by EST marker BE405348 and SSR marker Xwmc388 on chromosome 3AL with genetic distances of 2.2 and 4.6 cM, respectively. Comparative genomic analysis demonstrated that the orthologous genomic region of YrCH5389 covered 990 kb in rice, 640 kb in Brachypodium, and 890 kb in sorghum. Based on the locations of the markers, the resistance gene was located to chromosome deletion bin 3AL-0.85-1.00. Because there are no officially named stripe rust resistance genes on the 3AL chromosome, the YrCH5389 should be designated as a new resistance gene. These linkage markers could be useful for marker-assisted selection in wheat resistance breeding.
DOI: 10.6084/m9.figshare.6273719.v1
title: Molecular identification and mapping of a novel stripe rust resistance gene in wheat resistance line CH5389

description: ABSTRACT: Stripe rust, caused by Puccinia striiformis is one of the most destructive diseases of wheat worldwide. CH5389 is a wheat-Thinopyrum intermedium derived line conferring stripe rust resistance. Genetic analyses of seedlings of F2 populations and F2:3 families developed by crossing CH5389 and susceptible common wheat revealed that stripe rust resistance in CH5389 was controlled by a single dominant gene that was designated YrCH5389. Eight SSR and EST-PCR polymorphic markers on chromosome 3AL were identified in F2 population of CH5389/Taichung29. The YrCH5389 was flanked by EST marker BE405348 and SSR marker Xwmc388 on chromosome 3AL with genetic distances of 2.2 and 4.6 cM, respectively. Comparative genomic analysis demonstrated that the orthologous genomic region of YrCH5389 covered 990 kb in rice, 640 kb in Brachypodium, and 890 kb in sorghum. Based on the locations of the markers, the resistance gene was located to chromosome deletion bin 3AL-0.85-1.00. Because there are no officially named stripe rust resistance genes on the 3AL chromosome, the YrCH5389 should be designated as a new resistance gene. These linkage markers could be useful for marker-assisted selection in wheat resistance breeding.
DOI: 10.6084/m9.figshare.6273713
title: Reaction of lima bean accessions to Meloidogyne javanica

description: ABSTRACT Lima bean is one of the most popular leguminous plants in northeastern Brazil, with a strong socioeconomic influence on the regional communities. However, the low yield related to biotic and abiotic factors limits the increase in production. Among the biological agents, root-knot nematodes compromise its vegetative and productive development. In order to select genotypes resistant to Meloidogyne javanica, an experiment was carried out in a greenhouse and in a laboratory, in a completely randomized design, with fifteen lima bean accessions and five replicates. The reaction of lima bean accessions was evaluated through the infectivity and reproduction of M. javanica, as well as the main agronomic traits of the crop. A variation was observed in the resistance level among the accessions, with Lavandeira preta, Orelha de vó and Fava cearense being rated as very resistant, while Lavandeira vermelha was slightly resistant and the remaining accessions were all rated as susceptible.
DOI: 10.6084/m9.figshare.6273713.v1
title: Reaction of lima bean accessions to Meloidogyne javanica

description: ABSTRACT Lima bean is one of the most popular leguminous plants in northeastern Brazil, with a strong socioeconomic influence on the regional communities. However, the low yield related to biotic and abiotic factors limits the increase in production. Among the biological agents, root-knot nematodes compromise its vegetative and productive development. In order to select genotypes resistant to Meloidogyne javanica, an experiment was carried out in a greenhouse and in a laboratory, in a completely randomized design, with fifteen lima bean accessions and five replicates. The reaction of lima bean accessions was evaluated through the infectivity and reproduction of M. javanica, as well as the main agronomic traits of the crop. A variation was observed in the resistance level among the accessions, with Lavandeira preta, Orelha de vó and Fava cearense being rated as very resistant, while Lavandeira vermelha was slightly resistant and the remaining accessions were all rated as susceptible.
DOI: 10.6084/m9.figshare.6273710
title: Clay Mineralogy of Subtropical Soils under Long-Term Organic Fertilization in No-Tillage Systems

description: ABSTRACT: Organic fertilization effect on physical-chemical properties in no-tillage systems in tropical soils has been widely investigated, but little is known about the effects of this practice on the mineralogy of the clay fraction. This study aimed to evaluate the clay-fraction mineralogy of two subtropical soils, fertilized with organic residues in long-term no-tillage systems. An Alfisol fertilized with 0, 40, and 80 m3 ha-1 yr-1 pig slurry was evaluated for eight years, and an Oxisol with 0, 8, and 16 Mg ha-1 of wood shavings with pig slurry, for six years. Soil samples from the layers 0.00-0.04, 0.04-0.08, and 0.16-0.20 m were collected and subjected to chemical extractions with DCB and oxalate, X-ray diffractometry, and thermal analysis. No mineralogical changes in the clay fraction were observed in either the Alfisol or Oxisol. The chemical dissolution data indicated no significant differences among the tested treatments with regard to the re-precipitation of low-crystallinity oxides. However, the increase in zinc extracted by DCB and zinc extracted by oxalate in the treatments with residue application was clear. The crystallinity data efficiently indicated the effects of residue rates on soil mineralogy only in the Alfisol; the increasing slurry rates induced a reduction in the percentage of hematite, increase in the percentage of goethite, increase in the Gt/Gt+Hm ratio and decreased the mean crystal diameter of goethite and hematite.
DOI: 10.6084/m9.figshare.6273710.v1
title: Clay Mineralogy of Subtropical Soils under Long-Term Organic Fertilization in No-Tillage Systems

description: ABSTRACT: Organic fertilization effect on physical-chemical properties in no-tillage systems in tropical soils has been widely investigated, but little is known about the effects of this practice on the mineralogy of the clay fraction. This study aimed to evaluate the clay-fraction mineralogy of two subtropical soils, fertilized with organic residues in long-term no-tillage systems. An Alfisol fertilized with 0, 40, and 80 m3 ha-1 yr-1 pig slurry was evaluated for eight years, and an Oxisol with 0, 8, and 16 Mg ha-1 of wood shavings with pig slurry, for six years. Soil samples from the layers 0.00-0.04, 0.04-0.08, and 0.16-0.20 m were collected and subjected to chemical extractions with DCB and oxalate, X-ray diffractometry, and thermal analysis. No mineralogical changes in the clay fraction were observed in either the Alfisol or Oxisol. The chemical dissolution data indicated no significant differences among the tested treatments with regard to the re-precipitation of low-crystallinity oxides. However, the increase in zinc extracted by DCB and zinc extracted by oxalate in the treatments with residue application was clear. The crystallinity data efficiently indicated the effects of residue rates on soil mineralogy only in the Alfisol; the increasing slurry rates induced a reduction in the percentage of hematite, increase in the percentage of goethite, increase in the Gt/Gt+Hm ratio and decreased the mean crystal diameter of goethite and hematite.
DOI: 10.6084/m9.figshare.6273707
title: Permanent preservation area revegetated with tree species and green manures

description: ABSTRACT The main objective of this paper was to diagnose the situation of the study area, including the water body that is fueled by Córrego Samambaia. Measurements of height and diameter of tree species were made for 12 months. In addition, dam water samples were collected and the following parameters were analyzed: pH, conductivity, total phosphorus and heavy metals. In July and December of 2014, turbidity, color, biochemical oxygen demand (BOD) and dissolved oxygen (DO) analyzes were made. Physico-chemical analyses of soil were also carried out. Monitoring carried out from September 2013 to August 2014 showed some plants with good response in relation to the use of green manures, but others showed high mortality during the study, as the pequi. The competition between alien and native was reduced, which increased overall survival. Elements such as manganese showed no significant difference in all treatments during evaluation’s period. Macro and micropores values were similar, but for texture, the area showed some differences. For penetration resistance tests (PR) made in an area with and without green manure, the largest PR values were found in the one without legumes species. Parameters such as electric conductivity were stable during the 12 months of study. The results for DO and BOD are outside the recommended by the Resolution 357 of the National Environmental Council.
DOI: 10.6084/m9.figshare.6273707.v1
title: Permanent preservation area revegetated with tree species and green manures

description: ABSTRACT The main objective of this paper was to diagnose the situation of the study area, including the water body that is fueled by Córrego Samambaia. Measurements of height and diameter of tree species were made for 12 months. In addition, dam water samples were collected and the following parameters were analyzed: pH, conductivity, total phosphorus and heavy metals. In July and December of 2014, turbidity, color, biochemical oxygen demand (BOD) and dissolved oxygen (DO) analyzes were made. Physico-chemical analyses of soil were also carried out. Monitoring carried out from September 2013 to August 2014 showed some plants with good response in relation to the use of green manures, but others showed high mortality during the study, as the pequi. The competition between alien and native was reduced, which increased overall survival. Elements such as manganese showed no significant difference in all treatments during evaluation’s period. Macro and micropores values were similar, but for texture, the area showed some differences. For penetration resistance tests (PR) made in an area with and without green manure, the largest PR values were found in the one without legumes species. Parameters such as electric conductivity were stable during the 12 months of study. The results for DO and BOD are outside the recommended by the Resolution 357 of the National Environmental Council.
DOI: 10.6084/m9.figshare.6273704
title: Microstructural Evolution and Phase Transformation in Laser Cladding of Cr and Mo Powder on Grey Cast Iron: Mixture Design of Experiment (DOE)

description: Laser cladding on grey cast iron at high power processing was investigated for microstructural evolution and phase transformation to enhance surface properties. Cladding was designed using a mixture of DOE with peak power (Pp) and pulse repetition frequency (PRF), and a mixture component of Cr and Mo ratio as factors. Microstructural findings indicated absolute elimination of graphite phase from the clad zone, in conjunction with particles evolution occurrence. Meanwhile, Cr, Mo and Fe phases were detected on the clad surface, along with M-C carbide, retained austenite and MoFe formation. The clad surface with addition of Mo exhibited a high hardness value of 945.5 HV0.1 due to carbide formation. As a result of high peak power penetration into substrate surface, the depth range of clad zone was 53 to 131 µm. From the optimisation, the highest desirability is 82.3 %. Cladding with molybdenum powder addition was found to have produced minimum surface roughness, maximum depth and hardness of 9.14 µm, 110 µm and 891.1 HV0.1, respectively.
DOI: 10.6084/m9.figshare.6273704.v1
title: Microstructural Evolution and Phase Transformation in Laser Cladding of Cr and Mo Powder on Grey Cast Iron: Mixture Design of Experiment (DOE)

description: Laser cladding on grey cast iron at high power processing was investigated for microstructural evolution and phase transformation to enhance surface properties. Cladding was designed using a mixture of DOE with peak power (Pp) and pulse repetition frequency (PRF), and a mixture component of Cr and Mo ratio as factors. Microstructural findings indicated absolute elimination of graphite phase from the clad zone, in conjunction with particles evolution occurrence. Meanwhile, Cr, Mo and Fe phases were detected on the clad surface, along with M-C carbide, retained austenite and MoFe formation. The clad surface with addition of Mo exhibited a high hardness value of 945.5 HV0.1 due to carbide formation. As a result of high peak power penetration into substrate surface, the depth range of clad zone was 53 to 131 µm. From the optimisation, the highest desirability is 82.3 %. Cladding with molybdenum powder addition was found to have produced minimum surface roughness, maximum depth and hardness of 9.14 µm, 110 µm and 891.1 HV0.1, respectively.
DOI: 10.6084/m9.figshare.6273701
title: MgO Saturation Analisys of CaO-SiO2-FeO-MgO-Al2O3 Slag System

description: Electric arc furnace (EAF) slag has a range of functions, including improving energy efficiency through the "slag foaming" phenomenon. As such, the study of slag is important in EAFs. One of the factors that determine foaming quality and efficiency is the presence of solid particles. MgO saturation is highly significant since, in addition to ensuring the presence of solid particles, it also ensures chemical compatibility to minimize refractory consumption. The present study aims to examine MgO saturation, using the software FactSage v.6.4 for all calculations, in the CaO-SiO2-FeO-MgO-Al2O3 slag system and the influence of binary basicity, FeO and Al2O3 content on this system. Ternary diagrams revealed the influence of Al2O3 content on the liquid field and other phases present. Isothermal saturation diagrams (ISDs) were constructed, showing the same dual saturation points found in the ternary diagrams. The ISDs were validated by comparing the results of different heats in terms of the electrical energy consumption. The closer the heat is to the MgO saturation line, the less energy is consumed.
DOI: 10.6084/m9.figshare.6273701.v1
title: MgO Saturation Analisys of CaO-SiO2-FeO-MgO-Al2O3 Slag System

description: Electric arc furnace (EAF) slag has a range of functions, including improving energy efficiency through the "slag foaming" phenomenon. As such, the study of slag is important in EAFs. One of the factors that determine foaming quality and efficiency is the presence of solid particles. MgO saturation is highly significant since, in addition to ensuring the presence of solid particles, it also ensures chemical compatibility to minimize refractory consumption. The present study aims to examine MgO saturation, using the software FactSage v.6.4 for all calculations, in the CaO-SiO2-FeO-MgO-Al2O3 slag system and the influence of binary basicity, FeO and Al2O3 content on this system. Ternary diagrams revealed the influence of Al2O3 content on the liquid field and other phases present. Isothermal saturation diagrams (ISDs) were constructed, showing the same dual saturation points found in the ternary diagrams. The ISDs were validated by comparing the results of different heats in terms of the electrical energy consumption. The closer the heat is to the MgO saturation line, the less energy is consumed.
DOI: 10.6084/m9.figshare.6273698
title: Effect of Boride Incubation Time During the Formation of Fe2B Phase

description: Our present study focuses on the numerical simulation of the boronized layer growth kinetics on the iron substrate of the XC38 steel, the boronizing treatment is performed in a liquid medium composed of borax and silicon carbide. We mainly calculated the incubation time of the boronized layer formation. Aimed at estimating the boronizing treatment kinetics of XC38 steel, we initially used experimental data. The study was conducted to determine the law of borided layers growth and estimate the boron diffusion coefficient in the Fe2B layer. The diffusion coefficient obtained from this experiment is: D Fe 2 B = 1 . 388 × 10 − 4 exp − 207 . 8 × 10 3 j RT m 2 s − 1 In order to calculate the incubation time of the Fe2B layer, we adapted the mathematical model, which is based on the second law of Fick. This model takes into account the thermodynamic properties of the Fe-B phase diagram. The comparison of the results obtained by the simulation with those obtained experimentally verifies the validity of the theoretical study and a good agreement was obtained as well.
DOI: 10.6084/m9.figshare.6273698.v1
title: Effect of Boride Incubation Time During the Formation of Fe2B Phase

description: Our present study focuses on the numerical simulation of the boronized layer growth kinetics on the iron substrate of the XC38 steel, the boronizing treatment is performed in a liquid medium composed of borax and silicon carbide. We mainly calculated the incubation time of the boronized layer formation. Aimed at estimating the boronizing treatment kinetics of XC38 steel, we initially used experimental data. The study was conducted to determine the law of borided layers growth and estimate the boron diffusion coefficient in the Fe2B layer. The diffusion coefficient obtained from this experiment is: D Fe 2 B = 1 . 388 × 10 − 4 exp − 207 . 8 × 10 3 j RT m 2 s − 1 In order to calculate the incubation time of the Fe2B layer, we adapted the mathematical model, which is based on the second law of Fick. This model takes into account the thermodynamic properties of the Fe-B phase diagram. The comparison of the results obtained by the simulation with those obtained experimentally verifies the validity of the theoretical study and a good agreement was obtained as well.
DOI: 10.6084/m9.figshare.6273692
title: HCMV UL97 phosphotransferase gene mutations may be associated with antiviral resistance in immunocompromised patients in Belém, PA, Northern Brazil

description: Abstract INTRODUCTION: Human cytomegalovirus is one of the causes of opportunist infections in immunocompromised patients, and is triggered by factors such as state of viral latency, weakened immune responses, and development of antiviral resistance to ganciclovir, the only drug offered by the public health system in Brazil to treat the infection. The goal of this study was to identify mutations that may be associated with antiviral resistance in immunocompromised patients. METHODS: Molecular analysis was performed in 82 blood samples and subjected to genomic DNA extraction by a silica-based method. Three sequences of the HCMV UL97 gene, which encodes a phosphotransferase protein required for activation of ganciclovir, were amplified by polymerase chain reaction. Pyrosequencing methods were applied to one external 2096-bp segment DNA and two internal sequences between nucleotides 1087 to 1828 to detect mutations in this gene. RESULTS: Approximately 10% of sequences contained mutations between nucleotides 377 and 594, in conserved regions of the UL97 gene, leading to amino acid changes. Eleven coding mutations were identified, including changes leading to amino acid substitutions, E596K and S604F, which were observed in 100% of samples and are described for the first time in Brazil. In addition, one mutation (A594V) that is associated with ganciclovir resistance was detected in a kidney transplant patient. CONCLUSIONS: Further studies to detect mutations associated with HCMV resistance to antiviral drugs are required to demonstrate the need to increase the variety and availability of drugs used to treat viral infections in the public health care system in Brazil.
DOI: 10.6084/m9.figshare.6273692.v1
title: HCMV UL97 phosphotransferase gene mutations may be associated with antiviral resistance in immunocompromised patients in Belém, PA, Northern Brazil

description: Abstract INTRODUCTION: Human cytomegalovirus is one of the causes of opportunist infections in immunocompromised patients, and is triggered by factors such as state of viral latency, weakened immune responses, and development of antiviral resistance to ganciclovir, the only drug offered by the public health system in Brazil to treat the infection. The goal of this study was to identify mutations that may be associated with antiviral resistance in immunocompromised patients. METHODS: Molecular analysis was performed in 82 blood samples and subjected to genomic DNA extraction by a silica-based method. Three sequences of the HCMV UL97 gene, which encodes a phosphotransferase protein required for activation of ganciclovir, were amplified by polymerase chain reaction. Pyrosequencing methods were applied to one external 2096-bp segment DNA and two internal sequences between nucleotides 1087 to 1828 to detect mutations in this gene. RESULTS: Approximately 10% of sequences contained mutations between nucleotides 377 and 594, in conserved regions of the UL97 gene, leading to amino acid changes. Eleven coding mutations were identified, including changes leading to amino acid substitutions, E596K and S604F, which were observed in 100% of samples and are described for the first time in Brazil. In addition, one mutation (A594V) that is associated with ganciclovir resistance was detected in a kidney transplant patient. CONCLUSIONS: Further studies to detect mutations associated with HCMV resistance to antiviral drugs are required to demonstrate the need to increase the variety and availability of drugs used to treat viral infections in the public health care system in Brazil.
DOI: 10.6084/m9.figshare.6273686
title: Relationship between mental health and everyday cognitive failures: role of internalizing and externalizing symptoms

description: ABSTRACT Objective Cognitive failures are errors made in everyday life such as forgetting appointments, lack of words when talking, difficulties to memorize what is read, errors of orientation, among others. Internalizing and externalizing psychiatric symptoms may contribute to the occurrence of cognitive failures. The present study aims to evaluate how internalizing and externalizing symptoms contribute to the occurrence of everyday cognitive failures. Methods We evaluated 366 volunteers using the Cognitive Failure Questionnaire (CFQ), the Self-Reporting Questionnaire-20 (SRQ-20) as a measure of internalizing symptoms and the Adult Self-Report Scale (ASRS-18) as a measure of externalizing symptoms. We classified the participants based on the SRQ-20 and ASRS-18 cut-off scores to create four groups: controls, internalizing, externalizing, and mixed. We compared the groups by means of ANOVA and tested its associations with correlations. Results All scales showed strong correlations between each other (r &gt; 0.500, p &lt; 0.001). The comparison of the groups suggests that participants with clinical scores of internalizing and externalizing symptoms have more failures when compared to the control group (d = 0.86 and d = 1.00), but they do not present differences between themselves (d = 0.21). Participants of the mixed group presented more failures than the control group (d = 2.01), and the internalizing (d = 1.31) and externalizing (d = 1.05) groups. Conclusion Both internalizing and externalizing symptoms contribute to the occurrence of day-to-day cognitive failures. We also observed an additive effect of both symptoms.