DOI: 10.6084/m9.figshare.30951257
title: Dataset of the removal of Bt by Kumano

description: Dataset for the Bt removal submitted to AFE.Description of the data and file structureThese data were collected as part of a field experiment designed to evaluate the effects of invasive bumblebee (Bombus terrestris) removal on pollination dynamics, floral visitation behavior, and agricultural productivity in squash fields. Observations and manipulations were conducted between 2022, 2024 and 2025 in Obihiro, Hokkaido, Japan.<br>Files and variables<br>File: pol1c.csv<br>Description: This data was collected to assess the invasive bumblebee on fruit production (fruit weight and the number of seeds). Observations were conducted during the flowering season in 2022 (control) and 2024 (Bt removal) in Obihiro, Hokkaido, Japan.<br>Variables<br>flowerID : Flower ID (not used in the analysis).<br>fruit_weight: Weight of fruits (g).<br>fruited: Whether the focal flower fruited or not.<br>seed: Number of seeds in the fruits.<br>Year: 2022(control) or 2024(Bt removed).<br><br><br>File: pol1v.csv<br>Description:  This data was collected to assess the flower-visit behavior of bumblebees and the pollen deposition. Observations were conducted during the flowering season in 2022 (control) and 2024 (Bt removal) in Obihiro, Hokkaido, Japan.<br>Variables<br>flower_ID: Flower ID (not used in the analysis).<br>time_of_day: Time of day when the observation was conducted.<br>pollen: Number of pollen deposited on the stigma.<br>count: Number of individuals that visited the flower during monitoring.<br>total_visitation_time: Total visitation duration.<br><br><br>File: Removal_of__Bt.csv<br>Description: This dataset documents queen removal efforts of the invasive bumblebee Bombus terrestris as part of a field experiment conducted in 2022–2025 to evaluate the ecological consequences of removing this non-native pollinator from squash agroecosystems in Hokkaido, Japan.<br>Variables<br>Year: 2022, 2024, and 2025<br>Removal of Bt queens: Whether the year corresponds to the Bt queen removal year.<br>Number of removals: Number of removal days (days on which removal was conducted).<br>Participants: Total number of participants in each year who conducted Bt queen removals (persons).<br>Number of removed Bt queens: The results of Bt queen removal in each year.<br>Number of removed Bt workers: Number of Bt workers mistakenly captured.<br>Number of monitored Bt workers: Number of observed Bt workers on the squash flowers in 2022 and 2024.<br>Number of recorded Vespula: Number of observed Vespula individuals on the squash flowers in 2024.<br>Video footage hour: Total monitored duration using video.<br>Number of observed flowers: Total number of monitored flowers in each season.<br>Number of removed Bt queens in Hokkaido: Number of removed Bt queens in Hokkaido prefecture provided by HoBiCC (Hokkaido Biodiversity Conservation Center).<br>Number of removed Bt queens in Obihiro: Number of removed Bt queens in Obihiro city provided by HoBiCC.<br><br><br>File: single.csv<br>Description: This dataset was collected to assess the single visitation of the bumblebees on the pollen transfer. Observations were conducted during the 2022 flowering season in Obihiro, Hokkaido, Japan.<br>Variables<br>ID1: Flower ID (not used in the analysis).<br>day_since_start: Day index since the start of observations (Day 1 = 21 July).<br>time_of_day: Time of day when the observation was conducted.<br>pollen: Number of pollen deposited on the stigma.<br>fruited: Whether the focal flower fruited or not.<br>head: Head width of the visited individuals.<br><br><br>File: vespula.csv<br>Description: <br>Variables<br>flower_ID: Flower ID (not used in the analysis).<br>time_of_day: Time of day when the observation was conducted.<br>pollen: Number of pollen deposited on the stigma.<br>count: Number of individuals that visited the flower during monitoring.<br>total_visitation_time: Total visitation duration.<br><br><br>File: Temperature.csv<br>Description: Data on temperature and rainfall during the experimental periods were obtained from the university’s weather station.<br>Variables<br>Year: 2022 and 2024<br>Month: From April to August<br>Day: From 1 to 30 or 31.<br>Rain: Total rainfall for the day (mm/day).<br>Temp: Mean temperature for the day (°C, daily mean).<br><br><br>Code/software<br>All analysis was conducted on R. Used code was shown in the R_code_Kumano.txt file.