cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5666932.v1
title: Programa de Aquisição da Produção da Agricultura no Papa/DF: inovação gerencial e tecnológica em sistemas familiares, 2009-2016

description: Abstract: In Brazil, public food security policies include the National School Feeding Programme (PNAE), the Food Acquisition Program (PAA) and the Agricultural Production Acquisition Program of the Federal District (PAPA-DF). This study analyzed the impact of the latter (Papa/DF) on the adoption of management practices and of most appropriate production technologies by a group of farmers who participate in this policy, as these innovations have recognized relationship with their performance. The baseline was obtained from a 2009 data on these producers. In 2016, producers participating in Papa/DF were interviewed, applying a questionnaire likewise the one used in 2009, to identify changes in management and production processes, from 2009 to 2016. The results indicate positive changes in management innovation (planning and management processes) and technological innovation (adoption of recommended technologies) in these systems and on variables that can influence such results (production and producer characteristics).
DOI: 10.6084/m9.figshare.5666926
title: Increased peripheral blood TCD4+ counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy

description: BACKGROUND The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. OBJECTIVE To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. METHODS Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. FINDINGS In the CF patients, an expansion of the peripheral blood TCD4+ cells was observed during the treatment, and this persisted even after two years of antifungal treatment. In addition, these patients showed high serum levels of SP-D. CONCLUSION Our findings highlight the immunological changes CF patients undergo, during and after treatment, possibly due to the hypoxia triggered by pulmonary fibrosis and emphysema.
DOI: 10.6084/m9.figshare.5666926.v1
title: Increased peripheral blood TCD4+ counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy

description: BACKGROUND The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. OBJECTIVE To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. METHODS Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. FINDINGS In the CF patients, an expansion of the peripheral blood TCD4+ cells was observed during the treatment, and this persisted even after two years of antifungal treatment. In addition, these patients showed high serum levels of SP-D. CONCLUSION Our findings highlight the immunological changes CF patients undergo, during and after treatment, possibly due to the hypoxia triggered by pulmonary fibrosis and emphysema.
DOI: 10.6084/m9.figshare.5666923
title: Separation and Identification of Functional Groups of Molecules Responsible for Fluorescence of Biodiesel Using FTIR Spectroscopy and Principal Component Analysis

description: In order to separate and identify functional groups of molecules responsible for fluorescence compounds present in biodiesel, a column chromatography coupled with infrared spectroscopy and multivariate analysis was performed. A biodiesel sample was packed in a chromatographic column and the fractions obtained were used to perform the analyses. Before undergoing the separation process, the biodiesel sample was analyzed by light emitting diode (LED)-induced fluorescence and compared its spectrum with β-carotene and soybean oil patterns. The low cost and speed of analysis suggest that this technique can be used in the separation of biodiesel substances. The fluorescence emission spectra allowed identifying molecules such as β-carotene, in which the spectrum of its pattern exhibited fluorescence within a region ranging from 500 to 700 nm and chlorophyll molecules. When soybean oil is excited at around 405 nm, it features a fluorescent emission band within the region of 670 nm, which reveals the presence of chlorophyll. Infrared spectroscopy coupled with principal component analysis allowed to discriminate the fractions and to identify the functional groups of compounds present in the sample.
DOI: 10.6084/m9.figshare.5666923.v1
title: Separation and Identification of Functional Groups of Molecules Responsible for Fluorescence of Biodiesel Using FTIR Spectroscopy and Principal Component Analysis

description: In order to separate and identify functional groups of molecules responsible for fluorescence compounds present in biodiesel, a column chromatography coupled with infrared spectroscopy and multivariate analysis was performed. A biodiesel sample was packed in a chromatographic column and the fractions obtained were used to perform the analyses. Before undergoing the separation process, the biodiesel sample was analyzed by light emitting diode (LED)-induced fluorescence and compared its spectrum with β-carotene and soybean oil patterns. The low cost and speed of analysis suggest that this technique can be used in the separation of biodiesel substances. The fluorescence emission spectra allowed identifying molecules such as β-carotene, in which the spectrum of its pattern exhibited fluorescence within a region ranging from 500 to 700 nm and chlorophyll molecules. When soybean oil is excited at around 405 nm, it features a fluorescent emission band within the region of 670 nm, which reveals the presence of chlorophyll. Infrared spectroscopy coupled with principal component analysis allowed to discriminate the fractions and to identify the functional groups of compounds present in the sample.
DOI: 10.6084/m9.figshare.5666914
title: SPATIAL DISTRIBUTION OF SPONTANEOUS PRODUCTION OF Attalea maripa (Aubl) Mart. IN THE LOW TOCANTINS

description: ABSTRACT The production of biofuels from native Amazonian palm trees, belonging to the family of Arecaceae, like Attalea maripa (Aubl) Mart. (Maripa palm), are objects of studies of alternative energy sources for the biofuels production. Thus, the aim of this research was to evaluate the spatial dynamics of agronomic variables of Maripa palm crop production associated with the characteristics of the sampling sites in a spontaneous growth area in the Low Tocantins region. 100 palms of A. maripa were selected, of spontaneous growth and in production phase, on a 10 ha area in the municipality of Cametá / PA, in which the morpho-agronomical variables were collected (MIN, FIN, ABN, LN, DBH, AWAB and AFW), from January 2014 to June 2014. The semivariograms were produced for each variable, which presented a spatial dependence range of 75 meters to MIN, FIN, AWAB and AFW, 80 meters to LN and DBH and 98 meters to ABN. The area of reach found was 17,671.5 m² for MIN, FIN, AWAB and AFW variables, 20,106.2 m² for LN and DBH and 30,171.9 m² for ABN. The data of the variables were adjusted to the spherical model presenting determination coefficient (R²) that ranged from 0.57 to 0.99. The spatial dependence index (SDI) for MIN and FIN variables was 0.49 and 0.31, respectively, indicating moderate spatial dependence. For ABN, LN, DBH and AWAB variables ranged from 0.17 to 0.22, resulting in poor spatial dependence. The AFW variable presented SDI of 0.77. For the visual analysis of the spatial distribution of the studied variables, kriging maps were created.
DOI: 10.6084/m9.figshare.5666914.v1
title: SPATIAL DISTRIBUTION OF SPONTANEOUS PRODUCTION OF Attalea maripa (Aubl) Mart. IN THE LOW TOCANTINS

description: ABSTRACT The production of biofuels from native Amazonian palm trees, belonging to the family of Arecaceae, like Attalea maripa (Aubl) Mart. (Maripa palm), are objects of studies of alternative energy sources for the biofuels production. Thus, the aim of this research was to evaluate the spatial dynamics of agronomic variables of Maripa palm crop production associated with the characteristics of the sampling sites in a spontaneous growth area in the Low Tocantins region. 100 palms of A. maripa were selected, of spontaneous growth and in production phase, on a 10 ha area in the municipality of Cametá / PA, in which the morpho-agronomical variables were collected (MIN, FIN, ABN, LN, DBH, AWAB and AFW), from January 2014 to June 2014. The semivariograms were produced for each variable, which presented a spatial dependence range of 75 meters to MIN, FIN, AWAB and AFW, 80 meters to LN and DBH and 98 meters to ABN. The area of reach found was 17,671.5 m² for MIN, FIN, AWAB and AFW variables, 20,106.2 m² for LN and DBH and 30,171.9 m² for ABN. The data of the variables were adjusted to the spherical model presenting determination coefficient (R²) that ranged from 0.57 to 0.99. The spatial dependence index (SDI) for MIN and FIN variables was 0.49 and 0.31, respectively, indicating moderate spatial dependence. For ABN, LN, DBH and AWAB variables ranged from 0.17 to 0.22, resulting in poor spatial dependence. The AFW variable presented SDI of 0.77. For the visual analysis of the spatial distribution of the studied variables, kriging maps were created.
DOI: 10.6084/m9.figshare.5666911
title: Museum collections indicate bird defaunation in a biodiversity hotspot

description: Abstract Ipanema National Forest, southeastern Brazil, once contained 340 bird species. Forest cover suffered for centuries from log exploitation and, as a result, most of the remaining forests are now an impoverished subset of the original vegetation. We show how the bird community changed over time by comparing historical and recent records. Currently, 228 species can be recorded, for a compilation of 410 species, of which 359 are documented. Some 89 forest species with historical records failed to be detected in recent surveys. Of the 72 Atlantic Forest or Cerrado endemic species, no more than 29 (40%) are still found. The bird community changed from one which used to be related to coastline rain forests to another, which relates more to drier semideciduous forests of the interior.
DOI: 10.6084/m9.figshare.5666911.v1
title: Museum collections indicate bird defaunation in a biodiversity hotspot

description: Abstract Ipanema National Forest, southeastern Brazil, once contained 340 bird species. Forest cover suffered for centuries from log exploitation and, as a result, most of the remaining forests are now an impoverished subset of the original vegetation. We show how the bird community changed over time by comparing historical and recent records. Currently, 228 species can be recorded, for a compilation of 410 species, of which 359 are documented. Some 89 forest species with historical records failed to be detected in recent surveys. Of the 72 Atlantic Forest or Cerrado endemic species, no more than 29 (40%) are still found. The bird community changed from one which used to be related to coastline rain forests to another, which relates more to drier semideciduous forests of the interior.
DOI: 10.6084/m9.figshare.5666908
title: Exploiting Cork as Biosorbent Extraction Phase for Solid-Phase Microextraction to Determine 3-(4-Methylbenzylidene)camphor and 2-Ethylhexyl 4-(Dimethylamino)benzoate in River Water by Gas Chromatography-Mass Spectrometry

description: In this study, a method for the determination of 3-(4-methylbenzylidene)camphor (4-MBC) and 2-ethylhexyl 4-(dimethylamino)benzoate (OD-PABA) in river water samples employing cork as the SPME (solid-phase microextraction) fiber was developed. The extraction parameters optimized were pH, ionic strength and extraction time and temperature. For the extraction step, the sample pH was studied in univariate experiments while the extraction time, temperature and ionic strength were evaluated using a central composite design. The optimal extraction conditions were sample pH 4.0, extraction time 70 min, sample temperature 80 °C and sodium chloride concentration 6% (m v-1). The limits of quantification for 4-MBC and OD-PABA were 0.1 and 0.01 µg L-1, respectively. The linear correlation coefficients were greater than 0.97 for both analytes and the developed method showed recovery values between 67 and 107%. In an evaluation of the fiber-to-fiber reproducibility (fibers produced by different analysts), the relative standard deviation (RSD) was lower than 11% (n = 6).
DOI: 10.6084/m9.figshare.5666908.v1
title: Exploiting Cork as Biosorbent Extraction Phase for Solid-Phase Microextraction to Determine 3-(4-Methylbenzylidene)camphor and 2-Ethylhexyl 4-(Dimethylamino)benzoate in River Water by Gas Chromatography-Mass Spectrometry

description: In this study, a method for the determination of 3-(4-methylbenzylidene)camphor (4-MBC) and 2-ethylhexyl 4-(dimethylamino)benzoate (OD-PABA) in river water samples employing cork as the SPME (solid-phase microextraction) fiber was developed. The extraction parameters optimized were pH, ionic strength and extraction time and temperature. For the extraction step, the sample pH was studied in univariate experiments while the extraction time, temperature and ionic strength were evaluated using a central composite design. The optimal extraction conditions were sample pH 4.0, extraction time 70 min, sample temperature 80 °C and sodium chloride concentration 6% (m v-1). The limits of quantification for 4-MBC and OD-PABA were 0.1 and 0.01 µg L-1, respectively. The linear correlation coefficients were greater than 0.97 for both analytes and the developed method showed recovery values between 67 and 107%. In an evaluation of the fiber-to-fiber reproducibility (fibers produced by different analysts), the relative standard deviation (RSD) was lower than 11% (n = 6).
DOI: 10.6084/m9.figshare.5666905
title: Development and Validation of a Stability-Indicating Method for Perillyl Alcohol Incorporated in Poly(lactide-co-glycolide) Nanoparticles and Stress Degradation Studies

description: Perillyl alcohol has been studied in the treatment of cancer disease. However, its high toxicity is a drawback, which can be overcome by its incorporation in nanostructured systems. The aim of this work was to develop and validate a chromatographic method for determination of perillyl alcohol encapsulation efficiency in a polymeric nanoparticles formulation and evaluation of the presence of related degradation products. Perillyl alcohol was subjected to forced conditions of hydrolysis (acidic, alkaline and neutral), oxidation, photolysis and thermal stress, as suggested in the International Conference of Harmonization (ICH) guidelines. The drug showed significant degradation under acidic conditions. The degradation products could be adequately separated on an XBridge C18 column (100 × 2.1 mm, 3.5 µm) using isocratic elution (350 µL min−1) of water/acetonitrile (65 : 35, v/v) at 210 nm. Data from validation studies demonstrated that the method is selective, linear (coefficient of determination (R2) &gt; 0.999) over the range of 20.0-80.0 µg mL−1, precise (relative standard deviation (RSD) &lt; 2.0%), accurate (98.07 to 101.99%) and robust for minor changes. The method was successfully applied to determine the encapsulation efficiency of perillyl alcohol in polymeric nanoparticles, both for product development and for quality control purposes. After nanoparticles production, the presence of degradation products was not observed indicating that the single-emulsion solvent-evaporation technique used does not favor chemical degradation of the drug.
DOI: 10.6084/m9.figshare.5666905.v1
title: Development and Validation of a Stability-Indicating Method for Perillyl Alcohol Incorporated in Poly(lactide-co-glycolide) Nanoparticles and Stress Degradation Studies

description: Perillyl alcohol has been studied in the treatment of cancer disease. However, its high toxicity is a drawback, which can be overcome by its incorporation in nanostructured systems. The aim of this work was to develop and validate a chromatographic method for determination of perillyl alcohol encapsulation efficiency in a polymeric nanoparticles formulation and evaluation of the presence of related degradation products. Perillyl alcohol was subjected to forced conditions of hydrolysis (acidic, alkaline and neutral), oxidation, photolysis and thermal stress, as suggested in the International Conference of Harmonization (ICH) guidelines. The drug showed significant degradation under acidic conditions. The degradation products could be adequately separated on an XBridge C18 column (100 × 2.1 mm, 3.5 µm) using isocratic elution (350 µL min−1) of water/acetonitrile (65 : 35, v/v) at 210 nm. Data from validation studies demonstrated that the method is selective, linear (coefficient of determination (R2) &gt; 0.999) over the range of 20.0-80.0 µg mL−1, precise (relative standard deviation (RSD) &lt; 2.0%), accurate (98.07 to 101.99%) and robust for minor changes. The method was successfully applied to determine the encapsulation efficiency of perillyl alcohol in polymeric nanoparticles, both for product development and for quality control purposes. After nanoparticles production, the presence of degradation products was not observed indicating that the single-emulsion solvent-evaporation technique used does not favor chemical degradation of the drug.
DOI: 10.6084/m9.figshare.5666899
title: Antifungal Properties of High Efficient W/WO3 Electrodes Acting under UV-Vis and Visible Light and Chloride Medium

description: The present work investigates the use of W/WO3 electrodes prepared by electrochemical anodization applied in the photoelectrocatalytic disinfection of Candida parapsilosis using ultraviolet-visible (UV-Vis) and visible irradiation. The core objective of this work lies in describing a novel approach involving the use of chloride as supporting electrolyte, aiming at achieving a faster inactivation and towards understanding its behavior in water containing high chloride content. The best experimental conditions were found to be at pH 7.0 and 0.1 mol L-1 NaCl when the photoelectrode was biased at 1.5 V (vs. Ag/AgCl) illuminated by both UV-Vis and visible light. It is suggested that charges photogenerated on the electrode surface give rise preferably to HO•, known to be powerful oxidant that causes the total inactivation of the microorganism (1 min of treatment) while engendering around 84% of mineralization of organic matter released from the cell damage following 120 min of treatment.
DOI: 10.6084/m9.figshare.5666899.v1
title: Antifungal Properties of High Efficient W/WO3 Electrodes Acting under UV-Vis and Visible Light and Chloride Medium

description: The present work investigates the use of W/WO3 electrodes prepared by electrochemical anodization applied in the photoelectrocatalytic disinfection of Candida parapsilosis using ultraviolet-visible (UV-Vis) and visible irradiation. The core objective of this work lies in describing a novel approach involving the use of chloride as supporting electrolyte, aiming at achieving a faster inactivation and towards understanding its behavior in water containing high chloride content. The best experimental conditions were found to be at pH 7.0 and 0.1 mol L-1 NaCl when the photoelectrode was biased at 1.5 V (vs. Ag/AgCl) illuminated by both UV-Vis and visible light. It is suggested that charges photogenerated on the electrode surface give rise preferably to HO•, known to be powerful oxidant that causes the total inactivation of the microorganism (1 min of treatment) while engendering around 84% of mineralization of organic matter released from the cell damage following 120 min of treatment.
DOI: 10.6084/m9.figshare.5666896
title: Chiral Bistacrine Analogues: Synthesis, Cholinesterase Inhibitory Activity and a Molecular Modeling Approach

description: Cholinesterase enzymes are important targets for the therapy of Alzheimer’s disease. Tacrine-based dual binding site cholinesterases inhibitors are potential disease-modifying anti-Alzheimer drug candidates. In the present work, we described the synthesis of a series of chiral homo- and heterodimers of bis(7)-tacrine connected by a heptylene chain as a spacer with the methyl substituent at the C-3 position of the alicyclic region of tacrine nucleus and/or a chlorine atom attached to the C-6. Friedländer cyclocondensation between (R) or (S) 3-methylcyclohexanone prepared from monoterpene pulegone and o-aminobenzoic acids in the presence of POCl3 afford 9-chloroacridines as intermediates, which were used to the synthesis of homo- and heterodimers. All compounds demonstrated to be potent inhibitors of acetylcholinesterase (AChE) at low nanomolar concentration and showed selectivity for AChE over butyrylcholinesterase (BuChE). Furthermore, the affinity difference between enantiomeric bis(7)-tacrine analogues series indicated some degree of stereoselectivity in the active site of AChE for chiral bis-cognitin compounds.
DOI: 10.6084/m9.figshare.5666896.v1
title: Chiral Bistacrine Analogues: Synthesis, Cholinesterase Inhibitory Activity and a Molecular Modeling Approach

description: Cholinesterase enzymes are important targets for the therapy of Alzheimer’s disease. Tacrine-based dual binding site cholinesterases inhibitors are potential disease-modifying anti-Alzheimer drug candidates. In the present work, we described the synthesis of a series of chiral homo- and heterodimers of bis(7)-tacrine connected by a heptylene chain as a spacer with the methyl substituent at the C-3 position of the alicyclic region of tacrine nucleus and/or a chlorine atom attached to the C-6. Friedländer cyclocondensation between (R) or (S) 3-methylcyclohexanone prepared from monoterpene pulegone and o-aminobenzoic acids in the presence of POCl3 afford 9-chloroacridines as intermediates, which were used to the synthesis of homo- and heterodimers. All compounds demonstrated to be potent inhibitors of acetylcholinesterase (AChE) at low nanomolar concentration and showed selectivity for AChE over butyrylcholinesterase (BuChE). Furthermore, the affinity difference between enantiomeric bis(7)-tacrine analogues series indicated some degree of stereoselectivity in the active site of AChE for chiral bis-cognitin compounds.