cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.15468/dl.u355ct
title: Occurrence Download

description: A dataset containing 7 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Amphibalanus improvisus",
 "Country is Italy",
 "HasCoordinate is true"
 ]
}

 The dataset includes 7 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004782-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.dvjagx
title: Occurrence Download

description: A dataset containing 158 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Amphibalanus improvisus",
 "Country is Japan",
 "HasCoordinate is true"
 ]
}

 The dataset includes 158 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004783-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ur6482
title: Occurrence Download

description: A dataset containing 109682 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Achaeta geniculata, Aira macrantha, Koeleria albescens sylvatica, Koeleria alpigena mannagettae, Koeleria britannica, Koeleria cristata macrantha, Koeleria cristata pseudocristata, Koeleria cristata gracilis, Koeleria cristata macrantha, Koeleria cristata poaeformis, Koeleria cristata pseudocristata, Koeleria cristata violacea, Koeleria cristata violacea, Koeleria elatior glabrescens, Koeleria eminens, Koeleria glaucovirens jankae, Koeleria gracilis aurea, Koeleria gracilis biflora, Koeleria gracilis filifolia, Koeleria gracilis flaccida, Koeleria gracilis glabra, Koeleria gracilis pallida, Koeleria gracilis pubescens, Koeleria gracilis quadriflora, Koeleria gracilis triflora, Koeleria gracilis violascens, Koeleria gracilis helvetica, Koeleria gracilis mandshurica, Koeleria idahoensis idahoensis, Koeleria mannagettae, Koeleria nitida, Koeleria nitida colorata, Koeleria nitida congesta, Koeleria nitida filifolia, Koeleria nitida pubescens, Koeleria nitida nitida, Koeleria poaeformis, Koeleria polyantha californica, Koeleria pontarlieri, Koeleria pseudocristata pubescens, Koeleria pseudocristata pseudocristata, Koeleria pseudocristata pseudonitada, Koeleria robinsoniana robinsoniana, Koeleria supra-arenaria, Koeleria theodoriana, Poa cristata, Sesleria caerulea, Aira varia, Alopecurus varius, Cynosurus caeruleus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 {
 "not" : {
 "BasisOfRecord" : [
 "is one of (Fossil, Living Specimen, Machine Observation)"
 ]
 }
 },
 "Continent is Europe",
 "Year is greater than or equal to 1980",
 "Year is less than or equal to 2024"
 ]
}

 The dataset includes 109682 records from 429 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004741-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.tc6vyh
title: Occurrence Download

description: A dataset containing 100 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Clarias alluaudi)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 100 records from 17 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004784-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.5281/zenodo.15832097
title: Disproportionate infection by Eustrongylides sp. (Nematoda: Dioctophymatoidea: Dioctophymatidae) in the imperiled Harter's watersnake, Nerodia harteri

description: Data file, R Markdown file, and sequence alignments for the paper "Disproportionate infection by Eustrongylides sp. (nematoda: Dioctophymatoidea: Dioctophymatidae) in the imperiled Harter's watersnake, Nerodia harteri."
DOI: 10.5281/zenodo.15832096
title: Disproportionate infection by Eustrongylides sp. (Nematoda: Dioctophymatoidea: Dioctophymatidae) in the imperiled Harter's watersnake, Nerodia harteri

description: Data file, R Markdown file, and sequence alignments for the paper "Disproportionate infection by Eustrongylides sp. (nematoda: Dioctophymatoidea: Dioctophymatidae) in the imperiled Harter's watersnake, Nerodia harteri."
DOI: 10.15468/dl.dpvy86
title: Occurrence Download

description: A dataset containing 50574 species occurrences available in GBIF matching the query:
{
 "and" : [
 "ScientificName is Juniperus virginiana L.",
 "Year is greater than or equal to 1990",
 "Year is less than or equal to 2025",
 "HasCoordinate is true"
 ]
}

 The dataset includes 50574 records from 232 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004736-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.jf59j6
title: Occurrence Download

description: A dataset containing 8 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Abactochromis labrosus, Acanthocleithron chapini, Acanthopagrus berda, Acapoeta tanganicae, Acentrogobius audax, Acentrogobius nebulosus, Acentrogobius therezieni, Acipenser sturio, Afronandus sheljuzhkoi, Afronemacheilus abyssinicus, Agonostomus catalai, Agonostomus telfairii, Alburnus alburnus, Alcolapia alcalica, Alcolapia grahami, Alcolapia latilabris, Alcolapia ndalalani, Alestes ansorgii, Alestes baremoze, Alestes bouboni, Brycinus nurse, Alestes dentex, Alestes liebrechtsii, Alestes macrophthalmus, Alestes stuhlmannii, Alestopetersius bifasciatus, Alestopetersius brichardi, Alestopetersius caudalis, Alestopetersius compressus, Alestopetersius hilgendorfi, Alestopetersius leopoldianus, Alestopetersius nigropterus, Alestopetersius smykalai, Alestopetersius tumbensis, Allenbatrachus meridionalis, Alosa agone, Alosa algeriensis, Alosa alosa, Alosa fallax, Alticorpus geoffreyi, Alticorpus macrocleithrum, Alticorpus mentale, Alticorpus peterdaviesi, Alticorpus profundicola, Altolamprologus calvus, Altolamprologus compressiceps, Neolamprologus fasciatus, Amarginops platus, Ambassis ambassis, Ambassis dussumieri, Ambassis fontoynonti, Ambassis gymnocephalus, Ambassis natalensis, Ambassis urotaenia, Amphilius atesuensis, Amphilius athiensis, Amphilius brevis, Amphilius caudosignatus, Amphilius cryptobullatus, Amphilius dimonikensis, Amphilius grandis, Amphilius jacksonii, Amphilius kakrimensis, Amphilius korupi, Amphilius lamani, Amphilius lampei, Amphilius laticaudatus, Amphilius lentiginosus, Amphilius longirostris, Amphilius lujani, Amphilius maesii, Amphilius mamonekenensis, Amphilius natalensis, Amphilius opisthophthalmus, Amphilius platychir, Amphilius pulcher, Amphilius rheophilus, Amphilius uranoscopus, Amphilius zairensis, Anaecypris punica, Pseudophoxinus punicus, Ancharius fuscus, Ancharius griseus, Andersonia leptura, Anguilla anguilla, Anguilla bengalensis, Anguilla bicolor, Anguilla marmorata, Anguilla mossambica, Anomalochromis thomasi, Aphanius apodus)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((18.2494 -34.8049,19.1382 -35.0665,19.1382 -33.9401,17.8437 -33.9401,17.8729 -34.3757,18.2494 -34.8049))"
 ]
}

 The dataset includes 8 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004785-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.ubxsek
title: Occurrence Download

description: A dataset containing 86462 species occurrences available in GBIF matching the query:
{
 "and" : [
 "HasCoordinate is true",
 "TaxonKey is Clinopodium acinos"
 ]
}

 The dataset includes 86462 records from 952 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0004689-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/9g28a-0fs32
title: V. cholerae CK2188 delta-tcpR

description: Classical strain with ctxA deletionStrain name CK2188 (tcpR gene knonkout)Cell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2016-08-06</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Gabriela Kovacikova</p>
<strong>Purification / Growth Conditions / Treatment:</strong> O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-06-7/rawdata/</p>20160806_YWC218_10007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-06-7/rawdata/20160806_YWC218_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160806_YWC218_10007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-08-06-7/3dimage_76396/20160806_YWC218_10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/wea8j-gtg44
title: Oil well Actinobacterium

description: Grid box YWC210
description: <strong>Tilt Series Date:</strong> 2016-04-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 75.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Fixed cells received from Meng Wang/ Xiao-Lei Wu Lab at Peking University, China</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Firstly wash cells using PBS for 3 times, then add proper volum of 2.5% glutaraldehyde followed 1 hour room temp incubation (done in Peking), then send to Caltech for freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-11-14/rawdata/</p>20160410_YWC210_10014.mrc,  Tilt Series (Pixel Size 0.48 nm), 996.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-11-14/rawdata/20160410_YWC210_10014.mrc"
        > <i class="download icon"></i> Download </a></p>

        20160410_YWC210_10014_part35_14.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2016-04-11-14/3dimage_74192/20160410_YWC210_10014_part35_14.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/a6a1m-wyr64
title: LCV_Legionella pneumophila

description: THP1 cells were infected with Legionella wild type cells and thereafter phagosomes (LCV) were purified by sucrose density gradient method. Phagoseomes were fixed before shipment.
description: <strong>Tilt Series Date:</strong> 2017-06-01</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Phagosome isolation method as described by Joelle Gabay (Endnote).</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-46/3dimage_85004/</p>LCV_20170601q0012_part55_4.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-46/3dimage_85004/LCV_20170601q0012_part55_4.rec"
        > <i class="download icon"></i> Download </a></p>

        LCV_20170601q0012.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-01-46/rawdata/LCV_20170601q0012.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f2cv1-73v84
title: Lp02-fixed

description: WT Lp02 cells were fixed with GA and re-suspended in  PBS.
description: <strong>Tilt Series Date:</strong> 2017-11-20</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Shuman Lab</p>
<strong>Sample Preparation:</strong> 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-20-17/rawdata/</p>Legionella_fixed_201711200022.mrc,  Tilt Series (Pixel Size 0.39 nm), 2.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-20-17/rawdata/Legionella_fixed_201711200022.mrc"
        > <i class="download icon"></i> Download </a></p>

        Legionella_fixed_201711200022_part81_3.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-11-20-17/3dimage_90334/Legionella_fixed_201711200022_part81_3.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/m7vxz-jf515
title: perfusion_dcrp_fixed

description: This sample was fixed with 2.5% glutaraldehyde for 1 hour. The cells look sick, the outer membrane looks bad. Fixing for 1 hour turns out to be bad,need to fix for lesser time.
description: <strong>Tilt Series Date:</strong> 2015-08-18</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Shewanella oneidensis</p>
<strong>Strain:</strong> MR-1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Moh and Sahand</p>
<strong>Purification / Growth Conditions / Treatment:</strong> The sample was grown in perfusion chamber and fixed for 1hour with 2.5% glutaraldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1ul gold beads.</p>
<strong>Sample Preparation:</strong> The ice was too thick in the squares for which LM images were available, so could not correlate. Took tomograms of all bugs in a square.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-19/3dimage_60477/</p>perfusion_dcrp_atlas10019_full.rec,  Reconstruction (Pixel Size 2.4 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-19/3dimage_60477/perfusion_dcrp_atlas10019_full.rec"
        > <i class="download icon"></i> Download </a></p>

        perfusion_dcrp_atlas10019.mrc,  Tilt Series (Pixel Size 0.6 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-18-19/rawdata/perfusion_dcrp_atlas10019.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8tjhx-00417
title: Thermococcus koakarensis

description: Imaging Thermococcus koakarensis flagella
description: <strong>Tilt Series Date:</strong> 2016-09-20</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Thermococcus kodakarensis</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Standard Tk medium with starch and sulfur</p>
<strong>Sample Preparation:</strong> 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-20-8/rawdata/</p>atlas10007.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-20-8/rawdata/atlas10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10007_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-09-20-8/3dimage_79340/atlas10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/crtrc-2pr56
title: Saccharomyces cerevisiae - 6210

description: Raw data files of Saccharomyces cerevisiae - 6210
description: <strong>Tilt Series Date:</strong> 2017-04-21</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> Unspecified</p>
<strong>Strain:</strong> None</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Obtained from Dr. Markus Babst, University of Utah</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown overnight in YPD, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6.</p>
<strong>Sample Preparation:</strong> Cells were diluted to an OD of 0.2-0.25 in YPD, applied to the grid, blotted from behind manually and plunge frozen in liquid ethane-propane mix. ~200 nm lamellae were FIB milled before imaging by TEM.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-2/rawdata/</p>TS11_LamC.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-2/rawdata/TS11_LamC.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS11_trunc_4bin_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 356.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-2/3dimage_86275/TS11_trunc_4bin_DB.rec"
        > <i class="download icon"></i> Download </a></p>