DOI: 10.5061/dryad.2v00t
title: Data from: Metazoan mitochondrial gene sequence reference datasets for taxonomic assignment of environmental samples

description: Mitochondrial-encoded genes are increasingly targeted in studies using
 high-throughput sequencing approaches for characterizing metazoan
 communities from environmental samples (e.g., plankton, meiofauna,
 filtered water). Yet, unlike nuclear ribosomal RNA markers, there is to
 date no high-quality reference dataset available for taxonomic
 assignments. Here, we retrieved all metazoan mitochondrial gene sequences
 from GenBank, and then quality filtered and formatted the datasets for
 taxonomic assignments using taxonomic assignment tools. The reference
 datasets—‘Midori references’—are available for download at
 www.reference-midori.info. Two versions are provided: (I) Midori-UNIQUE
 that contains all unique haplotypes associated with each species and (II)
 Midori-LONGEST that contains a single sequence, the longest, for each
 species. Overall, the mitochondrial Cytochrome oxidase subunit I gene was
 the most sequence-rich gene. However, sequences of the mitochondrial large
 ribosomal subunit RNA and Cytochrome b apoenzyme genes were observed for a
 large number of species in some phyla. The Midori reference is compatible
 with some taxonomic assignment software. Therefore, automated
 high-throughput sequence taxonomic assignments can be particularly
 effective using these datasets.
description: MIDORI_LONGEST_1.1_A6_RDP.fastaMIDORI_LONGEST_1.1_A8_RDP.fastaMIDORI_LONGEST_1.1_COI_RDP.fastaMIDORI_LONGEST_1.1_COII_RDP.fastaMIDORI_LONGEST_1.1_COIII_RDP.fastaMIDORI_LONGEST_1.1_Cytb_RDP.fastaMIDORI_LONGEST_1.1_ND1_RDP.fastaMIDORI_LONGEST_1.1_ND2_RDP.fastaMIDORI_LONGEST_1.1_ND3_RDP.fastaMIDORI_LONGEST_1.1_ND4_RDP.fastaMIDORI_LONGEST_1.1_ND4L_RDP.fastaMIDORI_LONGEST_1.1_ND5_RDP.fastaMIDORI_LONGEST_1.1_ND6_RDP.fastaMIDORI_LONGEST_1.1_srRNA_RDP.fastaMIDORI_LONGEST_1.1_lrRNA_RDP.fastaMIDORI_LONGEST_1.1_A6_SPINGO.fastaMIDORI_LONGEST_1.1_A8_SPINGO.fastaMIDORI_LONGEST_1.1_COI_SPINGO.fastaMIDORI_LONGEST_1.1_COII_SPINGO.fastaMIDORI_LONGEST_1.1_COIII_SPINGO.fastaMIDORI_LONGEST_1.1_Cytb_SPINGO.fastaMIDORI_LONGEST_1.1_lrRNA_SPINGO.fastaMIDORI_LONGEST_1.1_ND1_SPINGO.fastaMIDORI_LONGEST_1.1_ND2_SPINGO.fastaMIDORI_LONGEST_1.1_ND3_SPINGO.fastaMIDORI_LONGEST_1.1_ND4_SPINGO.fastaMIDORI_LONGEST_1.1_ND4L_SPINGO.fastaMIDORI_LONGEST_1.1_ND5_SPINGO.fastaMIDORI_LONGEST_1.1_ND6_SPINGO.fastaMIDORI_LONGEST_1.1_srRNA_SPINGO.fastaMIDORI_UNIQUE_1.1_A6_RDP.fastaMIDORI_UNIQUE_1.1_A8_RDP.fastaMIDORI_UNIQUE_1.1_COI_RDP.fastaMIDORI_UNIQUE_1.1_COII_RDP.fastaMIDORI_UNIQUE_1.1_COIII_RDP.fastaMIDORI_UNIQUE_1.1_Cytb_RDP.fastaMIDORI_UNIQUE_1.1_lrRNA_RDP.fastaMIDORI_UNIQUE_1.1_ND1_RDP.fastaMIDORI_UNIQUE_1.1_ND2_RDP.fastaMIDORI_UNIQUE_1.1_ND3_RDP.fastaMIDORI_UNIQUE_1.1_ND4_RDP.fastaMIDORI_UNIQUE_1.1_ND4L_RDP.fastaMIDORI_UNIQUE_1.1_ND5_RDP.fastaMIDORI_UNIQUE_1.1_ND6_RDP.fastaMIDORI_UNIQUE_1.1_srRNA_RDP.fastaMIDORI_UNIQUE_1.1_A6_SPINGO.fastaMIDORI_UNIQUE_1.1_A8_SPINGO.fastaMIDORI_UNIQUE_1.1_COI_SPINGO.fastaMIDORI_UNIQUE_1.1_COII_SPINGO.fastaMIDORI_UNIQUE_1.1_COIII_SPINGO.fastaMIDORI_UNIQUE_1.1_Cytb_SPINGO.fastaMIDORI_UNIQUE_1.1_lrRNA_SPINGO.fastaMIDORI_UNIQUE_1.1_ND1_SPINGO.fastaMIDORI_UNIQUE_1.1_ND2_SPINGO.fastaMIDORI_UNIQUE_1.1_ND3_SPINGO.fastaMIDORI_UNIQUE_1.1_ND4_SPINGO.fastaMIDORI_UNIQUE_1.1_ND4L_SPINGO.fastaMIDORI_UNIQUE_1.1_ND5_SPINGO.fastaMIDORI_UNIQUE_1.1_ND6_SPINGO.fastaMIDORI_UNIQUE_1.1_srRNA_SPINGO.fasta