cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7336484
title: CHARACTERIZE OF PRODUCTION SYSTEMS AND MILK QUALITY OF PRODUCTION UNITS IN THE SEMIARID REGION OF CEARÁ

description: Abstract The aim of this study was to characterize the production systems and milk quality of eight milk production units (MPU) in the semiarid region of the state of Ceará, Brazil. Individual interviews were conducted through a questionnaire on the characteristics of the properties and production systems. Samples of milk and water were collected for a period of 12 months. Four MPUs were classified as semi-specialized and four as non-specialized. No differences were observed in milk composition between the two production systems, and both showed protein, fat, and solids-not-fat levels. Differences were observed for somatic cell count. The semi-specialized systems showed an average somatic cell and total bacterial count which is above the limit established by the 62nd Normative Instruction. All water samples showed presence of Escherichia coli. Somatic cell count was positively correlated (fat) and negatively (lactose). For total bacterial count, correlations were negative for fat, total solids, and somatic cell count. The microbiological quality of milk is not in accordance with the 62st and 7th Normative Instructions. The specialization of the production systems did not result in improved microbiological aspects of milk.
DOI: 10.6084/m9.figshare.7336484.v1
title: CHARACTERIZE OF PRODUCTION SYSTEMS AND MILK QUALITY OF PRODUCTION UNITS IN THE SEMIARID REGION OF CEARÁ

description: Abstract The aim of this study was to characterize the production systems and milk quality of eight milk production units (MPU) in the semiarid region of the state of Ceará, Brazil. Individual interviews were conducted through a questionnaire on the characteristics of the properties and production systems. Samples of milk and water were collected for a period of 12 months. Four MPUs were classified as semi-specialized and four as non-specialized. No differences were observed in milk composition between the two production systems, and both showed protein, fat, and solids-not-fat levels. Differences were observed for somatic cell count. The semi-specialized systems showed an average somatic cell and total bacterial count which is above the limit established by the 62nd Normative Instruction. All water samples showed presence of Escherichia coli. Somatic cell count was positively correlated (fat) and negatively (lactose). For total bacterial count, correlations were negative for fat, total solids, and somatic cell count. The microbiological quality of milk is not in accordance with the 62st and 7th Normative Instructions. The specialization of the production systems did not result in improved microbiological aspects of milk.
DOI: 10.6084/m9.figshare.6318590
title: ENDOPARASITES IN BIRDS OF GUARIBAS BIOLOGICAL RESERVE, ATLANTIC FOREST, PARAÍBA STATE, BRAZIL

description: Abstract This paper describes the findings on endoparasites in Atlantic forest birds at Guaribas Biological Reserve, Paraíba State, Northeast of Brazil. We captured 151 birds with mist nets in ten field expeditions between July 2010 and June 2011 and studied bird endoparasites through blood and excreta samples. Blood samples were collected from 131 birds and excreta were collected from 48 birds. In blood smears, only Haemoproteus columbae was detected in three Columbiformes (2.29%) of the species Columbina talpacoti. Fecal samples were collected from 48 birds, and eggs of nematodes and oocysts of coccidian were detected in 6.25% of the samples: Spirurida (Nematoda) in Lanio cristatus (Thraupidae); Strongyloides sp. (Nematoda) in Momotus momota (Momotidae) and Isospora sp. (Protozoa, Eimeriidae) in Neopelma pallescens (Pipridae). There is limited information about the parasites of wild birds in the remaining fragments of Atlantic forest in Northeast of Brazil; however, such knowledge can help understanding these host-parasite interactions.
DOI: 10.6084/m9.figshare.6318590.v1
title: ENDOPARASITES IN BIRDS OF GUARIBAS BIOLOGICAL RESERVE, ATLANTIC FOREST, PARAÍBA STATE, BRAZIL

description: Abstract This paper describes the findings on endoparasites in Atlantic forest birds at Guaribas Biological Reserve, Paraíba State, Northeast of Brazil. We captured 151 birds with mist nets in ten field expeditions between July 2010 and June 2011 and studied bird endoparasites through blood and excreta samples. Blood samples were collected from 131 birds and excreta were collected from 48 birds. In blood smears, only Haemoproteus columbae was detected in three Columbiformes (2.29%) of the species Columbina talpacoti. Fecal samples were collected from 48 birds, and eggs of nematodes and oocysts of coccidian were detected in 6.25% of the samples: Spirurida (Nematoda) in Lanio cristatus (Thraupidae); Strongyloides sp. (Nematoda) in Momotus momota (Momotidae) and Isospora sp. (Protozoa, Eimeriidae) in Neopelma pallescens (Pipridae). There is limited information about the parasites of wild birds in the remaining fragments of Atlantic forest in Northeast of Brazil; however, such knowledge can help understanding these host-parasite interactions.
DOI: 10.6084/m9.figshare.5980345
title: RETROSPECTIVE STUDY OF 98 CATS SUBMITTED TO ACUPUNCTURE ATTENDED AT A REHABILITATION AND CHRONIC PAIN CONTROL SERVICE

description: Abstract This is a retrospective survey with the aim of investigate the clinical cases of cats attended at a rehabilitation and chronic pain control service during 13 years. The aim of this study was to analyze the efficacy and methods of acupuncture, evaluate clinical evolution and the patient profile in 98 clinical charts. The clinical outcome data showed that 69.7% of patients improved after treatment with acupuncture, 16.7% had no significant response and 13.6% died. No patient had worsening, despite three of nine animals that died were due to progression of the primary disease treated only with acupuncture. The main indications for the use of acupuncture in cats at the clinic were neurological diseases - particularly trauma in the spinal cord and brain - where there was an improvement in 53.7% of the animals. Treatment was considered successful in neurological cases, when animals regained independent locomotion and in cases of spinal disease, when neurological scores changed for IV or V to I or II. Dry needle acupuncture was the most used technique for the treatment, with 94.7% of prevalence, followed by electroacupuncture (30.6%) and laserpuncture (21.4%). The mean number of acupuncture sessions were 6.97±14.97. It was concluded that acupuncture was efficient to treat most of the cats with different clinical conditions, showing that these species probably respond as well as dogs after acupuncture treatment.
DOI: 10.6084/m9.figshare.5980345.v1
title: RETROSPECTIVE STUDY OF 98 CATS SUBMITTED TO ACUPUNCTURE ATTENDED AT A REHABILITATION AND CHRONIC PAIN CONTROL SERVICE

description: Abstract This is a retrospective survey with the aim of investigate the clinical cases of cats attended at a rehabilitation and chronic pain control service during 13 years. The aim of this study was to analyze the efficacy and methods of acupuncture, evaluate clinical evolution and the patient profile in 98 clinical charts. The clinical outcome data showed that 69.7% of patients improved after treatment with acupuncture, 16.7% had no significant response and 13.6% died. No patient had worsening, despite three of nine animals that died were due to progression of the primary disease treated only with acupuncture. The main indications for the use of acupuncture in cats at the clinic were neurological diseases - particularly trauma in the spinal cord and brain - where there was an improvement in 53.7% of the animals. Treatment was considered successful in neurological cases, when animals regained independent locomotion and in cases of spinal disease, when neurological scores changed for IV or V to I or II. Dry needle acupuncture was the most used technique for the treatment, with 94.7% of prevalence, followed by electroacupuncture (30.6%) and laserpuncture (21.4%). The mean number of acupuncture sessions were 6.97±14.97. It was concluded that acupuncture was efficient to treat most of the cats with different clinical conditions, showing that these species probably respond as well as dogs after acupuncture treatment.
DOI: 10.6084/m9.figshare.6318632
title: CLINICAL AND LABORATORIAL ALTERATIONS AS INDICATORS FOR ANTI-HELMINTIC TREATMENT IN SHEEP EXPERIMENTALLY INFECTED WITH Haemonchus contortus

description: Abstract The intensity of anemia and hypoproteinemia are indicators of the seriousness of haemonchosis. The objective of this study was to verify the changes in weight, hematocrit, and plasma protein, as well as the parasite load, indicating the need for anthelmintic intervention in sheep experimentally infected with Haemonchus contortus. Fourteen crossbred Santa Inês male sheep were used, weighing 42.5 (± 7.5) kg, nematodes-free. The animals were divided into two groups of seven animals each. The group of infected animals received 10,000 H. contortus larvae each. All animals were monitored after 21, 28, 35, 42, 49, 56, 63, and 70 days post-infection and the following parameters were assessed: EPG, Famacha©, weight, total plasma proteins, and hematocrit. In the infected group, the EPG varied and peaked on the 42nd follow-up day. The hematocrit became significantly lower after the experimental infection. Famacha score© ranged from 1 to 2 and it was significantly higher at 21, 28, 35 and 42 days. No change was observed in plasma protein level and body weight in the control group. Infection with up to 10,000 larvae of H. contortus. and EPG up to 2500 did not significantly affect weight, hematocrit, and plasma protein of crossbred Santa Inês sheep with no need for anthelmintic intervention.
DOI: 10.6084/m9.figshare.6318632.v1
title: CLINICAL AND LABORATORIAL ALTERATIONS AS INDICATORS FOR ANTI-HELMINTIC TREATMENT IN SHEEP EXPERIMENTALLY INFECTED WITH Haemonchus contortus

description: Abstract The intensity of anemia and hypoproteinemia are indicators of the seriousness of haemonchosis. The objective of this study was to verify the changes in weight, hematocrit, and plasma protein, as well as the parasite load, indicating the need for anthelmintic intervention in sheep experimentally infected with Haemonchus contortus. Fourteen crossbred Santa Inês male sheep were used, weighing 42.5 (± 7.5) kg, nematodes-free. The animals were divided into two groups of seven animals each. The group of infected animals received 10,000 H. contortus larvae each. All animals were monitored after 21, 28, 35, 42, 49, 56, 63, and 70 days post-infection and the following parameters were assessed: EPG, Famacha©, weight, total plasma proteins, and hematocrit. In the infected group, the EPG varied and peaked on the 42nd follow-up day. The hematocrit became significantly lower after the experimental infection. Famacha score© ranged from 1 to 2 and it was significantly higher at 21, 28, 35 and 42 days. No change was observed in plasma protein level and body weight in the control group. Infection with up to 10,000 larvae of H. contortus. and EPG up to 2500 did not significantly affect weight, hematocrit, and plasma protein of crossbred Santa Inês sheep with no need for anthelmintic intervention.
DOI: 10.6084/m9.figshare.6318605
title: EPIDEMIOLOGY OF GASTROINTESTINAL HELMINTHS IN BUFFALOES

description: Abstract This study evaluated the prevalence and seasonality of natural infection by gastrointestinal parasites and the susceptibility of different dairy buffalo categories in Bandeirantes, state of Mato Grosso do Sul, Brazil. Forty-one Murrah buffaloes were divided into three categories, 11 suckling calves (0-8 months old), 10 weaned calves (8-18 months old), and 20 adult females over the age of 18 months. Adult animals were free from parasitism or showed very low parasite load. The suckling calves showed a peak of egg per gram of feces (EPG = 2038) in November, when the average rainfall was 69.3 mm and the temperature 25.95 ºC, while the lowest egg count (167) occurred in May, two months after the peak of 234.68 mm and 20.59 ºC. Weaned calves showed two peaks of EPG from 1288.89 and 875 in September and June, with rainfall of 41.45 and 18.4 mm, temperature 24.16 and 20.69 ºC, respectively. Significant (p = 0.01) and negative (r = -0.33) correlation between the EPG of suckling calves and precipitation was observed. The most common gastrointestinal parasites were Haemonchus sp. and Cooperia sp.
DOI: 10.6084/m9.figshare.6318605.v1
title: EPIDEMIOLOGY OF GASTROINTESTINAL HELMINTHS IN BUFFALOES

description: Abstract This study evaluated the prevalence and seasonality of natural infection by gastrointestinal parasites and the susceptibility of different dairy buffalo categories in Bandeirantes, state of Mato Grosso do Sul, Brazil. Forty-one Murrah buffaloes were divided into three categories, 11 suckling calves (0-8 months old), 10 weaned calves (8-18 months old), and 20 adult females over the age of 18 months. Adult animals were free from parasitism or showed very low parasite load. The suckling calves showed a peak of egg per gram of feces (EPG = 2038) in November, when the average rainfall was 69.3 mm and the temperature 25.95 ºC, while the lowest egg count (167) occurred in May, two months after the peak of 234.68 mm and 20.59 ºC. Weaned calves showed two peaks of EPG from 1288.89 and 875 in September and June, with rainfall of 41.45 and 18.4 mm, temperature 24.16 and 20.69 ºC, respectively. Significant (p = 0.01) and negative (r = -0.33) correlation between the EPG of suckling calves and precipitation was observed. The most common gastrointestinal parasites were Haemonchus sp. and Cooperia sp.
DOI: 10.6084/m9.figshare.6318602
title: ILLEGAL ANIMAL-ORIGIN PRODUCTS SEIZED IN BAGGAGE FROM INTERNATIONAL FLIGHTS AT SAO PAULO GUARULHOS AIRPORT (GRU / SBGR), BRAZIL

description: Abstract Air transportation is one of the most important means to introduce illegally imported animal-origin products into a country. Studies have demonstrated that these items pose a risk of disseminating diseases. São Paulo Guarulhos International Airport (GRU / SBGR) is the main international airport in Brazil in terms of people movement and it has the largest number of seizures of animal-origin products. The aim of the present work was to describe the dynamics of the seizure of illegally imported animal-origin products in baggage from international flight passengers at GRU / SBGR Airport in Brazil. Five hundred and eighty-nine different flights from 43 airlines, arriving from 117 countries were analyzed between 2006 and 2009. The total number of seized items increased from 2006 to 2009 and a single flight from France had the highest number of seizures, followed by flights from South Africa and Germany. Countries were grouped into regions or continents to facilitate the analysis. This grouping was based on historical and cultural ties rather than geographical aspects. Seafood was the most frequently seized product, followed by dairy products, as well as processed and raw meat.
DOI: 10.6084/m9.figshare.6318602.v1
title: ILLEGAL ANIMAL-ORIGIN PRODUCTS SEIZED IN BAGGAGE FROM INTERNATIONAL FLIGHTS AT SAO PAULO GUARULHOS AIRPORT (GRU / SBGR), BRAZIL

description: Abstract Air transportation is one of the most important means to introduce illegally imported animal-origin products into a country. Studies have demonstrated that these items pose a risk of disseminating diseases. São Paulo Guarulhos International Airport (GRU / SBGR) is the main international airport in Brazil in terms of people movement and it has the largest number of seizures of animal-origin products. The aim of the present work was to describe the dynamics of the seizure of illegally imported animal-origin products in baggage from international flight passengers at GRU / SBGR Airport in Brazil. Five hundred and eighty-nine different flights from 43 airlines, arriving from 117 countries were analyzed between 2006 and 2009. The total number of seized items increased from 2006 to 2009 and a single flight from France had the highest number of seizures, followed by flights from South Africa and Germany. Countries were grouped into regions or continents to facilitate the analysis. This grouping was based on historical and cultural ties rather than geographical aspects. Seafood was the most frequently seized product, followed by dairy products, as well as processed and raw meat.
DOI: 10.6084/m9.figshare.6318635
title: EFFECT OF SOMATOTROPIN AND THYROXINE ON THE IN VITRO DEVELOPMENT OF BOVINE PREANTRAL FOLLICLES

description: Abstract The aim of the study was to evaluate the effect of recombinant bovine somatotropin (rbST) and thyroxine (T4) on survival and growth of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovarian fragments were collected in local abattoirs and immediately fixed for classical histology and transmission electron microscopy (non-cultured control). The other fragments were then cultured in situ for seven days in minimum essential medium alone (MEM+ - cultured control) or in the presence of 1,000 ng/mL rbST and 20 ng/mL T4, isolated or associated. After seven days, there was a reduction (P&lt;0.05) in the percentage of normal follicles in MEM+ alone or with T4. In oocyte diameter, there was a reduction in MEM+ alone. There was no influence (P&gt;0.01) of the medium used on the follicular diameter of the PAOF cultured for seven days. Ultrastructural analysis showed cell damage. In conclusion, the presence of rbST maintains the rate of morphologically normal follicles during the culture for seven days (observed by optical microscopy), but it does not exert beneficial effects on its ultrastructural integrity and oocyte and follicular growth.
DOI: 10.6084/m9.figshare.6318635.v1
title: EFFECT OF SOMATOTROPIN AND THYROXINE ON THE IN VITRO DEVELOPMENT OF BOVINE PREANTRAL FOLLICLES

description: Abstract The aim of the study was to evaluate the effect of recombinant bovine somatotropin (rbST) and thyroxine (T4) on survival and growth of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovarian fragments were collected in local abattoirs and immediately fixed for classical histology and transmission electron microscopy (non-cultured control). The other fragments were then cultured in situ for seven days in minimum essential medium alone (MEM+ - cultured control) or in the presence of 1,000 ng/mL rbST and 20 ng/mL T4, isolated or associated. After seven days, there was a reduction (P&lt;0.05) in the percentage of normal follicles in MEM+ alone or with T4. In oocyte diameter, there was a reduction in MEM+ alone. There was no influence (P&gt;0.01) of the medium used on the follicular diameter of the PAOF cultured for seven days. Ultrastructural analysis showed cell damage. In conclusion, the presence of rbST maintains the rate of morphologically normal follicles during the culture for seven days (observed by optical microscopy), but it does not exert beneficial effects on its ultrastructural integrity and oocyte and follicular growth.
DOI: 10.6084/m9.figshare.6318623
title: EVALUATION OF OXIDATIVE STRESS IN PLASMA OF DAIRY COWS WITH MASTITIS

description: Abstract Bovine mastitis is associated with an endogenous antibacterial response mediated by the production of reactive species. However, excess oxidative reactions can trigger cellular apoptosis, aggravating the clinical state of the animals. In this context, the objective of this study was to evaluate the plasma redox response of dairy cows with and without mastitis submitted or not to antibiotic therapy. The cows were divided into control group (G1), cows without mastitis; group G2, cows with mastitis without antimicrobial treatment; group G3, cows with mastitis treated with antibiotics. Blood samples were collected after the first milking of the morning. The existence of lipoperoxidation (LPO) and the levels of carbonylated proteins (CPs), reduced glutathione (GSH), ascorbic acid (ASA), and uric acid (UA) were analyzed. The G3 animals showed increase in LPO and CPs. In all groups GSH levels were unchanged. Plasma ASA and AU values were decreased in the G2 and G3 groups. The results demonstrated that antimicrobial treatment seems to aggravate the oxidative damages present in bovine mastitis, reinforcing the importance of the search for therapeutic alternatives in order to minimize this effect.
DOI: 10.6084/m9.figshare.6318623.v1
title: EVALUATION OF OXIDATIVE STRESS IN PLASMA OF DAIRY COWS WITH MASTITIS

description: Abstract Bovine mastitis is associated with an endogenous antibacterial response mediated by the production of reactive species. However, excess oxidative reactions can trigger cellular apoptosis, aggravating the clinical state of the animals. In this context, the objective of this study was to evaluate the plasma redox response of dairy cows with and without mastitis submitted or not to antibiotic therapy. The cows were divided into control group (G1), cows without mastitis; group G2, cows with mastitis without antimicrobial treatment; group G3, cows with mastitis treated with antibiotics. Blood samples were collected after the first milking of the morning. The existence of lipoperoxidation (LPO) and the levels of carbonylated proteins (CPs), reduced glutathione (GSH), ascorbic acid (ASA), and uric acid (UA) were analyzed. The G3 animals showed increase in LPO and CPs. In all groups GSH levels were unchanged. Plasma ASA and AU values were decreased in the G2 and G3 groups. The results demonstrated that antimicrobial treatment seems to aggravate the oxidative damages present in bovine mastitis, reinforcing the importance of the search for therapeutic alternatives in order to minimize this effect.
DOI: 10.6084/m9.figshare.6318584
title: PREOPERATIVE ELECTROCARDIOGRAPHIC STUDY OF DOGS AT THE VETERINARY HOSPITAL OF PONTIFÍCIA UNIVERSIDADE CATÓLICA DO PARANÁ

description: Abstract Electrocardiogram is considered a substantial instrument in the clinical evaluation of patients with heart disease. This tool can be used to determine the origin and frequency of the impulse as well as the cardiac conduction disorders. The aim of this study was to evaluate the frequency and type of electrocardiographic abnormalities in the immediate preoperative period of 60 dogs seen in the surgery department of the Veterinary Hospital of Pontifícia Universidade Católica do Paraná - PUCPR, campus Toledo, between September 2015 and June 2017, and to analyze its correlation with the different breeds, sex, age, and weight of each patient. Alterations in the preoperative electrocardiogram have been shown to be frequent and their occurrence is independent of age, breed, size, and sex of the animal. The most frequent findings in the present study were the following: respiratory sinus arrhythmia (30%), atrial fibrillation (16,7%), sinus tachycardia (10%), increase in R wave amplitude (10%), and ST segment depression (10%). Therefore, electrocardiography proved to be an auxiliary diagnostic method of great relevance in the dogs' preoperative period, guaranteeing greater anesthesia safety and lower risks of cardiovascular complications.