cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/hrwfx-f6v55
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-09-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~15 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5 µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-66/3dimage_73274/</p>20150909_AK_pMT15_30015_SIRT_2K.rec,  Reconstruction (Pixel Size 0.0 nm), 14.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-66/3dimage_73274/20150909_AK_pMT15_30015_SIRT_2K.rec"
        > <i class="download icon"></i> Download </a></p>

        20150909_AK_pMT15_30015.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-66/rawdata/20150909_AK_pMT15_30015.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/vj23p-60f15
title: Agrobacter

description: Mutant Agro cells, delVirB (whole of the VirB system deleted). Grown on induction plate pH5.8 in exactly similar way as the AD1484 strain.
description: <strong>Tilt Series Date:</strong> 2015-12-25</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 195.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSFtomo</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow at 19C for 4 days on induction plate. Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD348_delVirB) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-25-18/3dimage_71855/</p>agrobacter_20003_full.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-25-18/3dimage_71855/agrobacter_20003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        agrobacter_20003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-12-25-18/rawdata/agrobacter_20003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.hfz2de
title: Occurrence Download

description: A dataset containing 1237 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is China",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Carpinus cordata"
 ]
}

 The dataset includes 1237 records from 19 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006198-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/e1pc8-12067
title: Agro_AD1484_piliprep7_20150916

description: AD1484:A348dB/pAD2079, virB on a multicopy-plasmidFor pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate. Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to process the data. Grids were little contaminated and data is not very high quality.
description: <strong>Tilt Series Date:</strong> 2015-09-15</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Patch Tracking</p>
<strong>Collaborators and Roles:</strong> Pili prep from induced strain (AD1484) was done  by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> For pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: incubate at 100% humidity for at least 15 min, 4µl sample, blot force 6, blot time 3 sec. Wait time 30 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to process the data.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-15-3/rawdata/</p>AD1484_piliprep_70002.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-15-3/rawdata/AD1484_piliprep_70002.mrc"
        > <i class="download icon"></i> Download </a></p>

        AD1484_piliprep_70002.rec,  Reconstruction (Pixel Size 1.92 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-15-3/3dimage_67880/AD1484_piliprep_70002.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/4vbbg-aff34
title: Agro_AD1484_piliprep7_20150916

description: AD1484:A348dB/pAD2079, virB on a multicopy-plasmidFor pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate. Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to process the data. Grids were little contaminated and data is not very high quality.
description: <strong>Tilt Series Date:</strong> 2015-09-15</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Patch Tracking</p>
<strong>Collaborators and Roles:</strong> Pili prep from induced strain (AD1484) was done  by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the F30, Caltech.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> For pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: incubate at 100% humidity for at least 15 min, 4µl sample, blot force 6, blot time 3 sec. Wait time 30 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to process the data.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-15-12/3dimage_67894/</p>AD1484_piliprep_70009.rec,  Reconstruction (Pixel Size 1.92 nm), 890.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-15-12/3dimage_67894/AD1484_piliprep_70009.rec"
        > <i class="download icon"></i> Download </a></p>

        AD1484_piliprep_70009.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-09-15-12/rawdata/AD1484_piliprep_70009.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/f1qjp-nv546
title: Agro_AD1231

description: AD1231: pAD1361/A136; Carbenicillin 50;  virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.
description: <strong>Tilt Series Date:</strong> 2015-08-03</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 155.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grow culture in AB medium (pH 7) at 30C. For induction, use 375 µl of a decent ON culture to inoculate 5 ml AB mes pH 5.8 medium containing 100 µM Acetosyringone and antibiotic (Cb 50). Grow at 20C for 18 hr. Concentrate cells 5 times by centrifuging. Freeze cells.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK III, MK IV was not working) settings: 100% humidity, 4µl sample, 5% BSA treated gold. Blot offset -5, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-28/3dimage_60032/</p>20150803_1231_10025_part121_21.rec,  Reconstruction (Pixel Size 1.92 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-28/3dimage_60032/20150803_1231_10025_part121_21.rec"
        > <i class="download icon"></i> Download </a></p>

        20150803_1231_10025.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-03-28/rawdata/20150803_1231_10025.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/c1cgq-4mx27
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletion.Cell harbors pMT5 plasmid (inducible toxT).
description: <strong>Tilt Series Date:</strong> 2015-12-19</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, place the tube on bench for ~20 min, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~20 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-64/3dimage_71680/</p>20151219_YWC206_10064_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-64/3dimage_71680/20151219_YWC206_10064_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20151219_YWC206_10064.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-12-19-64/rawdata/20151219_YWC206_10064.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/gk6kt-pne41
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-09-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~15 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5 µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-55/3dimage_61282/</p>20150909_AK_pMT15_30004_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-55/3dimage_61282/20150909_AK_pMT15_30004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        20150909_AK_pMT15_30004.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-55/rawdata/20150909_AK_pMT15_30004.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8r4mw-kpt05
title: C.crescentus, no FtsZ ring

description: Caulobacter crescentus NA1000, single cell, free-living, none-dividing. no FtsZ ring
description: <strong>Tilt Series Date:</strong> 2015-02-17</p>
<strong>Data Taken By:</strong> Qing Yao</p>
<strong>Species / Specimen:</strong> Caulobacter crescentus</p>
<strong>Strain:</strong> NA1000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> C. crescentus was from Yi-Wei's cryo-stock and was grown in PYE medium overnight at 30°c 250 rpm. In the next days' morning, 1/20 subculture into the M2G medium. When OD600 reaches 0.48, 1ml medium was spun down and the resuspension was 100 ul for cryo-grid preparation.</p>
<strong>Sample Preparation:</strong> Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 120/A2. defocus: -10um. magnification: 22500</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-02-17-41/rawdata/</p>ZMatlas20005.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-02-17-41/rawdata/ZMatlas20005.mrc"
        > <i class="download icon"></i> Download </a></p>

        ZMatlas20005.rec,  Reconstruction (Pixel Size 1.92 nm), 889.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-02-17-41/3dimage_52096/ZMatlas20005.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/khn86-gwb49
title: V. cholerae RT4236 (refreshed)

description: Classical strain with ctxA deletionCell harbors pMT5 plasmid (inducible toxT)
description: <strong>Tilt Series Date:</strong> 2015-09-09</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> O395-N1</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Ronald K. Taylor</p>
<strong>Purification / Growth Conditions / Treatment:</strong> V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~15 min before mixing with gold and freezing.</p>
<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 3.5 µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-38/3dimage_61248/</p>20150909_AK_pMT15_20026_part121_20.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-38/3dimage_61248/20150909_AK_pMT15_20026_part121_20.rec"
        > <i class="download icon"></i> Download </a></p>

        20150909_AK_pMT15_20026.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2015-09-09-38/rawdata/20150909_AK_pMT15_20026.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/s6es4-11891
title: Coxiella_burnetii_ wtACCMD_pH4d5

description: Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-5 culture. Cells grown in acidic media ACCMD and pH 4.75
description: <strong>Tilt Series Date:</strong> 2017-10-17</p>
<strong>Data Taken By:</strong> Debnath Ghosal</p>
<strong>Species / Specimen:</strong> Coxiella burnetii</p>
<strong>Strain:</strong> Nine Mile phase II clone 4</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -7.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.</p>
<strong>Sample Preparation:</strong> Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-17-48/rawdata/</p>Cb_d5pH4_20171017b0021.mrc,  Tilt Series (Pixel Size 0.39 nm), 1.7 GB  
        
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        Cb_d5pH4_20171017b0021_part61_14.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
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