cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.21257131.v1
title: Pharmacogenetics of HIV therapy: State of the art in Latin American countries

description: Abstract The use of combined antiretroviral therapy (cART) has resulted in a remarkable reduction in morbidity and mortality of people living with HIV worldwide. Nevertheless, interindividual variations in drug response often impose a challenge to cART effectiveness. Although personalized therapeutic regimens may help overcome incidence of adverse reactions and therapeutic failure attributed to host factors, pharmacogenetic studies are often restricted to a few populations. Latin American countries accounted for 2.1 million people living with HIV and 1.4 million undergoing cART in 2020-21. The present review describes the state of art of HIV pharmacogenetics in this region and highlights that such analyses remain to be given the required relevance. A broad analysis of pharmacogenetic markers in Latin America could not only provide a better understanding of genetic structure of these populations, but might also be crucial to develop more informative dosing algorithms, applicable to non-European populations.
DOI: 10.6084/m9.figshare.21257129.v1
title: Acute phase protein and vitamin D concentration in dogs with multicentric lymphoma

description: Abstract This study aimed to evaluate the serum concentration of vitamin D (25-Hydroxyvitamin D) and acute phase proteins (APPs; alpha-1 acid glycoprotein, haptoglobin, transferrin, ceruloplasmin, albumin, IgA, IgG and alpha-1 - antitrypsin) as potential biomarkers for prognostic and therapy response in dogs with multicentric lymphoma submitted to the CHOP (Cyclophosphamide, Doxorubicin, Vincristine and Prednisone) chemotherapy protocol. Thirteen dogs with multicentric lymphoma classified as high grade by cytology were included in the treatment group (GL), while ten healthy dogs were included in the control group (GC). Serum was collected in the weeks T0, T5 and T10 of CHOP chemotherapy protocol, for the GL group, and in a single collection, for the GC group. All the collected samples were evaluated for the APPs and vitamin D concentrations through electrophoresis and chemiluminescence methods, respectively. Diagnostic and staging tests were performed for all the dogs in the GL group, and included cytopathology, histopathology and immunohistochemistry of the affected lymph node. Of these dogs, 9 achieved a complete response and 4 a partial response to the treatment. Data analysis was performed with the R software. The results demonstrated that serum concentrations of IgA, haptoglobin and α1-acid glycoprotein were significantly different between the groups and also between the different chemotherapy times analyzed (p&lt;0.05), indicating that these proteins can be considered as sensitive biomarkers for lymphoma in dogs. Furthermore, the α1-acid glycoprotein showed prognostic value for the disease, with 63% specificity. However, vitamin D concentration was not correlated with prognosis of the dogs with lymphoma.
DOI: 10.6084/m9.figshare.21257131
title: Pharmacogenetics of HIV therapy: State of the art in Latin American countries

description: Abstract The use of combined antiretroviral therapy (cART) has resulted in a remarkable reduction in morbidity and mortality of people living with HIV worldwide. Nevertheless, interindividual variations in drug response often impose a challenge to cART effectiveness. Although personalized therapeutic regimens may help overcome incidence of adverse reactions and therapeutic failure attributed to host factors, pharmacogenetic studies are often restricted to a few populations. Latin American countries accounted for 2.1 million people living with HIV and 1.4 million undergoing cART in 2020-21. The present review describes the state of art of HIV pharmacogenetics in this region and highlights that such analyses remain to be given the required relevance. A broad analysis of pharmacogenetic markers in Latin America could not only provide a better understanding of genetic structure of these populations, but might also be crucial to develop more informative dosing algorithms, applicable to non-European populations.
DOI: 10.6084/m9.figshare.21257129
title: Acute phase protein and vitamin D concentration in dogs with multicentric lymphoma

description: Abstract This study aimed to evaluate the serum concentration of vitamin D (25-Hydroxyvitamin D) and acute phase proteins (APPs; alpha-1 acid glycoprotein, haptoglobin, transferrin, ceruloplasmin, albumin, IgA, IgG and alpha-1 - antitrypsin) as potential biomarkers for prognostic and therapy response in dogs with multicentric lymphoma submitted to the CHOP (Cyclophosphamide, Doxorubicin, Vincristine and Prednisone) chemotherapy protocol. Thirteen dogs with multicentric lymphoma classified as high grade by cytology were included in the treatment group (GL), while ten healthy dogs were included in the control group (GC). Serum was collected in the weeks T0, T5 and T10 of CHOP chemotherapy protocol, for the GL group, and in a single collection, for the GC group. All the collected samples were evaluated for the APPs and vitamin D concentrations through electrophoresis and chemiluminescence methods, respectively. Diagnostic and staging tests were performed for all the dogs in the GL group, and included cytopathology, histopathology and immunohistochemistry of the affected lymph node. Of these dogs, 9 achieved a complete response and 4 a partial response to the treatment. Data analysis was performed with the R software. The results demonstrated that serum concentrations of IgA, haptoglobin and α1-acid glycoprotein were significantly different between the groups and also between the different chemotherapy times analyzed (p&lt;0.05), indicating that these proteins can be considered as sensitive biomarkers for lymphoma in dogs. Furthermore, the α1-acid glycoprotein showed prognostic value for the disease, with 63% specificity. However, vitamin D concentration was not correlated with prognosis of the dogs with lymphoma.
DOI: 10.6084/m9.figshare.21257134
title: In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis

description: BACKGROUND Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest. OBJECTIVE To determine the in vitro activity of S. terebinthifolius against S. brasiliensis. METHODS Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied. FINDINGS The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity. MAIN CONCLUSIONS Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis.
DOI: 10.6084/m9.figshare.21257134.v1
title: In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis

description: BACKGROUND Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest. OBJECTIVE To determine the in vitro activity of S. terebinthifolius against S. brasiliensis. METHODS Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied. FINDINGS The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity. MAIN CONCLUSIONS Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis.
DOI: 10.6084/m9.figshare.21257135
title: Juices from 'Bordô' and 'BRS Cora' grapes grown in an organic production system in the Serra do Sudeste region

description: Abstract The objective of this work was to evaluate the physicochemical composition of juices from the 'Bordô' and 'BRS Cora' grapes grown in an organic production system in the Serra do Sudeste region, in Southern Brazil, as well as to compare the cultivar results with data from other producing regions and with the requirements of the Brazilian regulation for whole grape juice. The used grapes came from a commercial vineyard, especifically from the 2016-2017, 2017-2018, and 2018-2019 production cycles. The juices were prepared by the steam drag method and evaluated for their physicochemical composition. The fruit showed suitable values for the parameters necessary for grape juice production. In general, the juices showed bioactive compounds contents similar to those obtained in other regions. The 'Bordô' juices evaluated in the three production cycles showed significant differences for several variables, which were attributed to the influence of weather on grape maturity. The juices of 'BRS Cora' showed high-consistency qualitative results even under the very distinct meteorological conditions of the three production cycles. The juices comply with the current Brazilian regulation for whole grape juice, except for soluble solids, which are severely lowered by the used extraction technique.
DOI: 10.6084/m9.figshare.21257135.v1
title: Juices from 'Bordô' and 'BRS Cora' grapes grown in an organic production system in the Serra do Sudeste region

description: Abstract The objective of this work was to evaluate the physicochemical composition of juices from the 'Bordô' and 'BRS Cora' grapes grown in an organic production system in the Serra do Sudeste region, in Southern Brazil, as well as to compare the cultivar results with data from other producing regions and with the requirements of the Brazilian regulation for whole grape juice. The used grapes came from a commercial vineyard, especifically from the 2016-2017, 2017-2018, and 2018-2019 production cycles. The juices were prepared by the steam drag method and evaluated for their physicochemical composition. The fruit showed suitable values for the parameters necessary for grape juice production. In general, the juices showed bioactive compounds contents similar to those obtained in other regions. The 'Bordô' juices evaluated in the three production cycles showed significant differences for several variables, which were attributed to the influence of weather on grape maturity. The juices of 'BRS Cora' showed high-consistency qualitative results even under the very distinct meteorological conditions of the three production cycles. The juices comply with the current Brazilian regulation for whole grape juice, except for soluble solids, which are severely lowered by the used extraction technique.
DOI: 10.6084/m9.figshare.21257138.v1
title: Prediction of the natural distribution, habitat and conservation of Stryphnodendron pulcherrimum (Willd.) Hochr. in response to global climate change

description: ABSTRACT Stryphnodendron pulcherrimum is a species used medicinally among traditional Amazonian communities for its bactericidal activity and anti-inflammatory properties. Despite being adapted to rustic environments, there is no information regarding how climate change might affect the species occurrence. The present study aimed to evaluate the natural distribution of S. pulcherrimum in the current period and how its potential geographic distribution may be affected in response to future climate change scenarios in Brazilian phytogeographic domains. A total of 19 bioclimatic variables were used from the WorldClim database. Four algorithm models (Climate Space Model, Envelope Score, Niche Mosaic and Environmental Distance - present) and one software (Open Modeller - future) were used to verify the potential occurrence of S. pulcherrimum in five Brazilian domains (Amazon, Cerrado, Caatinga, Atlantic Forest and Pantanal) and three intervals (2009-2019 - present; 2020-2050 and 2051-2070 - future). There were losses of areas favorable to the occurrence of S. pulcherrimum in the Amazon, Cerrado and Pantanal, and global climate change may affect its natural distribution especially in the Atlantic Forest and Amazon. In the Amazon, the species may be totally extinct, in the worst scenario, by 2070.
DOI: 10.6084/m9.figshare.21257138
title: Prediction of the natural distribution, habitat and conservation of Stryphnodendron pulcherrimum (Willd.) Hochr. in response to global climate change

description: ABSTRACT Stryphnodendron pulcherrimum is a species used medicinally among traditional Amazonian communities for its bactericidal activity and anti-inflammatory properties. Despite being adapted to rustic environments, there is no information regarding how climate change might affect the species occurrence. The present study aimed to evaluate the natural distribution of S. pulcherrimum in the current period and how its potential geographic distribution may be affected in response to future climate change scenarios in Brazilian phytogeographic domains. A total of 19 bioclimatic variables were used from the WorldClim database. Four algorithm models (Climate Space Model, Envelope Score, Niche Mosaic and Environmental Distance - present) and one software (Open Modeller - future) were used to verify the potential occurrence of S. pulcherrimum in five Brazilian domains (Amazon, Cerrado, Caatinga, Atlantic Forest and Pantanal) and three intervals (2009-2019 - present; 2020-2050 and 2051-2070 - future). There were losses of areas favorable to the occurrence of S. pulcherrimum in the Amazon, Cerrado and Pantanal, and global climate change may affect its natural distribution especially in the Atlantic Forest and Amazon. In the Amazon, the species may be totally extinct, in the worst scenario, by 2070.
DOI: 10.6084/m9.figshare.21257140
title: Soil greenhouse gases emissions in a goat production system in the Brazilian semiarid region

description: ABSTRACT In the climate change scenario, studying greenhouse gases (GHG) emissions and measures of mitigation in the Caatinga biome are strategic and may provide a basis for mitigation plans. This study aimed to evaluate the soil CO2, CH4 and N2O fluxes, as well as determining an annual baseline for GHG emissions, in a reference site of silvopastoral production system in the Brazilian semiarid region, in order to provide subsidies for future studies on GHG emissions mitigation. The GHG fluxes were monitored over one year, in a buffel grass pasture and in grazed and native Caatinga areas, which are components of a long-term silvopastoral system. The CO2 fluxes ranged from -19.98 to 179.12 mg m-2 h-1 of CO2-C, CH4 fluxes from -76.21 to 113.87 µg m-2 h-1 of CH4-C, N2O fluxes from -1,043.12 to 471.37 µg m-2 h-1 of N2O-N and the soil moisture was the main factor limiting the GHG fluxes. The total emissions converted to CO2-equivalent in the anthropized areas were lower than in the native area (65 % for the buffel grass pasture and 741 % for the grazed Caatinga). Therefore, it is possible to afrm that the GHG soil emissions from grazed areas in the Caatinga biome are not as high as in the native Caatinga, what is an important indication of the environmental sustainability of the evaluated silvopastoral system.
DOI: 10.6084/m9.figshare.21257140.v1
title: Soil greenhouse gases emissions in a goat production system in the Brazilian semiarid region

description: ABSTRACT In the climate change scenario, studying greenhouse gases (GHG) emissions and measures of mitigation in the Caatinga biome are strategic and may provide a basis for mitigation plans. This study aimed to evaluate the soil CO2, CH4 and N2O fluxes, as well as determining an annual baseline for GHG emissions, in a reference site of silvopastoral production system in the Brazilian semiarid region, in order to provide subsidies for future studies on GHG emissions mitigation. The GHG fluxes were monitored over one year, in a buffel grass pasture and in grazed and native Caatinga areas, which are components of a long-term silvopastoral system. The CO2 fluxes ranged from -19.98 to 179.12 mg m-2 h-1 of CO2-C, CH4 fluxes from -76.21 to 113.87 µg m-2 h-1 of CH4-C, N2O fluxes from -1,043.12 to 471.37 µg m-2 h-1 of N2O-N and the soil moisture was the main factor limiting the GHG fluxes. The total emissions converted to CO2-equivalent in the anthropized areas were lower than in the native area (65 % for the buffel grass pasture and 741 % for the grazed Caatinga). Therefore, it is possible to afrm that the GHG soil emissions from grazed areas in the Caatinga biome are not as high as in the native Caatinga, what is an important indication of the environmental sustainability of the evaluated silvopastoral system.
DOI: 10.6084/m9.figshare.21257143
title: Alteration of the organic matter fractions in soil cultivated with sugarcane in the Cerrado-Amazon transition zone

description: ABSTRACT The use of agroindustrial wastes, such as vinasse, may alter the soil chemical and physical characteristics. This study aimed to evaluate the total organic carbon and total nitrogen in a soil and the distribution of carbon and nitrogen in the sand and clay granulometric fractions, in three management systems (with and without vinasse application and with burning for harvest, in comparison with primary/native forest coverage), regarding fertilization and harvesting of sugarcane, in the Midwest region of Brazil. The use of organic wastes in the sugarcane production system may promote, even in a short time frame, conservation and improvement of the soil quality by the addition and maintenance of organic carbon. The use of vinasse favors the increase of the total organic carbon and total nitrogen in the soil, in the clay fraction, at all evaluated depths. The system with burning results in lower total carbon levels in the sand fraction, in comparison with the clay fraction, and, even with the sugarcane harvest employing burning, the most recalcitrant organic matter is associated with the clay fraction.
DOI: 10.6084/m9.figshare.21257143.v1
title: Alteration of the organic matter fractions in soil cultivated with sugarcane in the Cerrado-Amazon transition zone

description: ABSTRACT The use of agroindustrial wastes, such as vinasse, may alter the soil chemical and physical characteristics. This study aimed to evaluate the total organic carbon and total nitrogen in a soil and the distribution of carbon and nitrogen in the sand and clay granulometric fractions, in three management systems (with and without vinasse application and with burning for harvest, in comparison with primary/native forest coverage), regarding fertilization and harvesting of sugarcane, in the Midwest region of Brazil. The use of organic wastes in the sugarcane production system may promote, even in a short time frame, conservation and improvement of the soil quality by the addition and maintenance of organic carbon. The use of vinasse favors the increase of the total organic carbon and total nitrogen in the soil, in the clay fraction, at all evaluated depths. The system with burning results in lower total carbon levels in the sand fraction, in comparison with the clay fraction, and, even with the sugarcane harvest employing burning, the most recalcitrant organic matter is associated with the clay fraction.
DOI: 10.6084/m9.figshare.21257149
title: Optimization of blueberry flour processing and anthocyanin extraction

description: Abstract The objective of this work was to determine the ideal conditions for the processing of flour and extraction of anthocyanins from blueberries (Vaccinium corymbosum). A central composite design (CCD) with response surfaces was used. For the processing of blueberry flour, different dehydration temperatures (53ºC-67ºC) and times (43.18-48.82 hours) were used. The extraction of anthocyanins was performed with ethanol solutions (15-85% v/v) at different pH values (1.0-4.0). ). The total monomeric anthocyanin content was 1,538.39±25.63 mg 100 g-1 (0.89 desirability value) for fresh blueberries in the optimal extraction condition (53.5% ethanol, 2.5 pH) and 3,101.04±11.57 mg 100 g-1 (0.96 desirability value) for blueberry flour in the optimum processing condition (53ºC, 46 hours). The optimized extraction and dehydration of blueberries provide high levels of anthocyanins in the samples.
DOI: 10.6084/m9.figshare.21257149.v1
title: Optimization of blueberry flour processing and anthocyanin extraction

description: Abstract The objective of this work was to determine the ideal conditions for the processing of flour and extraction of anthocyanins from blueberries (Vaccinium corymbosum). A central composite design (CCD) with response surfaces was used. For the processing of blueberry flour, different dehydration temperatures (53ºC-67ºC) and times (43.18-48.82 hours) were used. The extraction of anthocyanins was performed with ethanol solutions (15-85% v/v) at different pH values (1.0-4.0). ). The total monomeric anthocyanin content was 1,538.39±25.63 mg 100 g-1 (0.89 desirability value) for fresh blueberries in the optimal extraction condition (53.5% ethanol, 2.5 pH) and 3,101.04±11.57 mg 100 g-1 (0.96 desirability value) for blueberry flour in the optimum processing condition (53ºC, 46 hours). The optimized extraction and dehydration of blueberries provide high levels of anthocyanins in the samples.
DOI: 10.6084/m9.figshare.21257147
title: STUDIES ON EXTERNAL VOCALIC SANDHI PROCESSES – EMPHASIS ON ELISION – IN OLD PORTUGUESE

description: The main objectives of this study are the mapping and analysis of external vocalic sandhi processes in Portuguese religious songs (Cantigas de Santa Maria) by Alfonso X, the Wise (13th century). The sandhi processes that occur through the juxtaposition of words constitute elision, diphthongisation, crasis (MASSINI-CAGLIARI, 2005, for Old Portuguese). Through this work and an unexplored corpus (especially regarding linguistic themes - SNOW, 1987; MASSINI-CAGLIARI, 2005), we intend to contribute to the achievement of deeper knowledge of the history of Portuguese prosody in the period when it was recognised as an “independent” language from Latin.
DOI: 10.6084/m9.figshare.21257147.v1
title: STUDIES ON EXTERNAL VOCALIC SANDHI PROCESSES – EMPHASIS ON ELISION – IN OLD PORTUGUESE

description: The main objectives of this study are the mapping and analysis of external vocalic sandhi processes in Portuguese religious songs (Cantigas de Santa Maria) by Alfonso X, the Wise (13th century). The sandhi processes that occur through the juxtaposition of words constitute elision, diphthongisation, crasis (MASSINI-CAGLIARI, 2005, for Old Portuguese). Through this work and an unexplored corpus (especially regarding linguistic themes - SNOW, 1987; MASSINI-CAGLIARI, 2005), we intend to contribute to the achievement of deeper knowledge of the history of Portuguese prosody in the period when it was recognised as an “independent” language from Latin.