cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7482719
title: Serum biological indicators in free-living pampas deer (Ozotoceros bezoarticus bezoarticus, Linnaeus 1758) of the Pantanal Sul-Mato-Grossense

description: ABSTRACT The objective of the present study is to establish the reference intervals of different serum analytes in free - living Ozotoceros bezoarticus bezoarticus. Blood samples were drawn from the 54 females and 14 males, adults, captured in the Pantanal of Mato Grosso do Sul. A determination of total calcium, albumin, total cholesterol, creatinine, glucose, phosphorus, total protein, triglycerides, and serum urea concentrations and an activity of the enzymes alanine aminotransferase, aspartate aminotransferase, creatine kinase MB fraction and alkaline phosphatase were performed in automated equipment. Values of globulins, total calcium and proportions among analytes were performed by means of mathematical calculations. This is a first description of the reference parameters for serum biochemistry of free - living pampas deer in the Pantanal of Mato Grosso do Sul. Sample size and distribution of data indicate that the mean values obtained are representative and can be used for a health monitoring, nutritional status, and diagnosis of diseases in this population stratum.
DOI: 10.6084/m9.figshare.7482719.v1
title: Serum biological indicators in free-living pampas deer (Ozotoceros bezoarticus bezoarticus, Linnaeus 1758) of the Pantanal Sul-Mato-Grossense

description: ABSTRACT The objective of the present study is to establish the reference intervals of different serum analytes in free - living Ozotoceros bezoarticus bezoarticus. Blood samples were drawn from the 54 females and 14 males, adults, captured in the Pantanal of Mato Grosso do Sul. A determination of total calcium, albumin, total cholesterol, creatinine, glucose, phosphorus, total protein, triglycerides, and serum urea concentrations and an activity of the enzymes alanine aminotransferase, aspartate aminotransferase, creatine kinase MB fraction and alkaline phosphatase were performed in automated equipment. Values of globulins, total calcium and proportions among analytes were performed by means of mathematical calculations. This is a first description of the reference parameters for serum biochemistry of free - living pampas deer in the Pantanal of Mato Grosso do Sul. Sample size and distribution of data indicate that the mean values obtained are representative and can be used for a health monitoring, nutritional status, and diagnosis of diseases in this population stratum.
DOI: 10.7284/102742
title: INS (Inertial Navigation System) data collected during research cruise MGL1113 using a Applanix POS/MV-320 V3 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7482698
title: Resveratrol Attenuates LPS-induced Apoptosis via Inhibiting NF- κ B Activity in Chicken Peripheral Lymphocyte Cultures

description: ABSTRACT This study aims to investigate whether resveratrol (RES) could inhibit NF-κB activity and protect in vitro cultured chicken lymphocytes from apoptosis induced by continuous LPS stimulation. Blood lymphocytes of chickens were collected and cultured. LPS was added to the samples of treatment group, while the same volume of normal saline (NS) was supplemented to those of the control group. In the treatment groups, four different concentrations of RES (0, 20, 40, and 80 µg/ml) were admixed. Then, the following indicators were tested: lymphocyte apoptotic rate, lymphocyte viability, NF-κB activity, TNF-a level, reactive oxygen species (ROS), and expression of apoptosis-related proteins (Fas/FasL and Caspase-8). The results indicated that the application of different concentrations of RES could significantly increase the viability of lymphocytes and decrease the apoptotic rate (p&lt;0.05) after continuous stimulations by LPS. The activity of NF-κB levels of TNF-a and ROS in the RES treatment subgroups was significantly decreased in comparison with that in the RES-blank subgroup (p&lt;0.05). The cells in the treatment group that had been treated with 20, 40 and 80 μl/ml of RES exhibited a significantly lower Fas and Caspase-8 expression level than that in the RES-blank subgroup (p&lt;0.05). These findings revealed that RES could substantially diminish the concentrations of ROS and TNF-a, down-regulate NF-κB activity, decrease the expression of Fas and Caspase-8, stimulate the activity of peripheral lymphocytes, and lower the rate of their apoptosis, induced by continuous LPS stimulation in chicken lymphocyte cultures.
DOI: 10.6084/m9.figshare.7482701
title: Organic Egg Production, Egg Quality, Calcium Utilization, and Digestibility in Laying Hens Fed with Mushroom (Flammulina velutipes) Stem Waste

description: ABSTRACT The possibility of using mushroom (Flammulina velutipes) stem waste (FVW) in layer diets was explored in this study. A total of 180, 40-week-old ISA Brown laying hens were randomly assigned to 5 groups, with 6 replications of 6 birds for each group. Layers were fed a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4%FVW; and 6% FVW. No significant differences (p&gt;0.05) were observed on hen daily egg production, egg weight, egg mass, unmarketable eggs, feed intake, feed conversion ratio, and live weight among experimental groups. Haugh unit was found higher (p&lt;0.05) in FVW fed groups than in antibiotic fed groups. Shape index, shell weight, shell thickness, yolk weight, yolk index, yolk weight and albumen weight were not affected (p&gt;0.05) by feeding FVW in this study. Yolk color was found higher (p&lt;0.05) in FVW fed groups than in control and antibiotic groups. The shell color was higher (p&lt;0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups. Calcium retention and calcium in egg shell deposition were higher (p&lt;0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups respectively. Apparent nutrient retention for dry matter, crude protein, crude fat, and organic matter were higher (p&lt;0.05) in FVW fed groups than in control and antibiotic groups. FVW at 6% level in layer ration can be used for organic eggs production which would be safe and sound for human consumption.
DOI: 10.6084/m9.figshare.7482701.v1
title: Organic Egg Production, Egg Quality, Calcium Utilization, and Digestibility in Laying Hens Fed with Mushroom (Flammulina velutipes) Stem Waste

description: ABSTRACT The possibility of using mushroom (Flammulina velutipes) stem waste (FVW) in layer diets was explored in this study. A total of 180, 40-week-old ISA Brown laying hens were randomly assigned to 5 groups, with 6 replications of 6 birds for each group. Layers were fed a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4%FVW; and 6% FVW. No significant differences (p&gt;0.05) were observed on hen daily egg production, egg weight, egg mass, unmarketable eggs, feed intake, feed conversion ratio, and live weight among experimental groups. Haugh unit was found higher (p&lt;0.05) in FVW fed groups than in antibiotic fed groups. Shape index, shell weight, shell thickness, yolk weight, yolk index, yolk weight and albumen weight were not affected (p&gt;0.05) by feeding FVW in this study. Yolk color was found higher (p&lt;0.05) in FVW fed groups than in control and antibiotic groups. The shell color was higher (p&lt;0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups. Calcium retention and calcium in egg shell deposition were higher (p&lt;0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups respectively. Apparent nutrient retention for dry matter, crude protein, crude fat, and organic matter were higher (p&lt;0.05) in FVW fed groups than in control and antibiotic groups. FVW at 6% level in layer ration can be used for organic eggs production which would be safe and sound for human consumption.
DOI: 10.6084/m9.figshare.7482692
title: Survival evaluation of bacterial indicators and viruses during thermal drying of sewage sludge in a greenhouse

description: ABSTRACT The aim of the study was to evaluate the thermic drying of sewage sludge in agricultural greenhouse, in order to inactivate bacterial and virus, and to evaluate the behavior of somatic coliphages as indicator of these organisms. Each volume of excess sewage sludge discarded from UASB reactor, drained in a drying bed for 14 days and directed to thermic drying treatment in greenhouse corresponded to a batch (B) of sludge. Between February and November 2015, nine batches (L1 to L9) were treated for an average of 13 weeks. Most batches kept the pH between 5.0-6.0. After about 60 days of treatment, all batches showed humidity below 10%, except B3 and B4 (10-25%). The time required to reach 90% of total solids ranged between 40-50 (B1 to B7), 60 (B5, B6) and 85-100 (B2, B3 and B4) days. On average, levels of E. coli reduced to &lt;1x103 NMP/g TS after 50-60 days of treatment. The detection of somatic coliphages was low and eventual. Tests of inoculation showed rapid decay of virus (Sabin 1 strain poliovirus), but somatic coliphage Phi X174 was more persistent in the three temperatures evaluated (greenhouse, laboratory controlled 30 and 60°C). The thermic drying treatment of sewage sludge in agricultural greenhouse was presented as simple and efficient in obtaining granulated and dried product, and in reducing bacterial contamination levels given the parameter set as safe for agricultural use under the Brazilian law.
DOI: 10.6084/m9.figshare.7482695
title: The career advancement challenge faced by female executives in Peruvian organisations

description: Abstract The percentage of women in Peru's workforce overall has been growing at a steady rate, but there are still few women are seen in executive positions. This qualitative study describes the challenges in women's professional career advancement in a country in which the cultural pattern of business is dominated by men and where specific roles have been assigned to women. In-depth interviews were conducted with 24 professionals, 11 women and 13 men. The interviews were analysed using qualitative data analysis software. The results show that family responsibilities were the main challenge for female executives' career advancement. Moreover, family responsibilities were linked to other challenges, such as physical mobility, educational attainment, professional experience and informal networks. Family responsibilities can be considered a transcultural challenge to the career advancement of female executives. Finally, the study shows that a country's culture is an important challenge to career advancement, as in the Peruvian context where women are still considered responsible for attending to the family and are assigned a fundamental role in raising the children.
DOI: 10.6084/m9.figshare.7482710.v1
title: ON THE ICONICITY OF THE Y-HANDSHAPE

description: ABSTRACT As part of a two-year research project, the study examines the diagrammatic iconicity of the Y-handshape of two non-cognate sign languages; the American Sign Language and Greek Sign Language. In a sample of sixty-four signs, and through a close reading approach, it demonstrates the association of the specific handshape with real-world referents that have simultaneously a round and angular form (e.g. cylinder, cone), or only an angular/linear shape. It also shows its historic association with the ancient traditional sign mano cornuta , addressing its metonymy in meanings relating to quantity, earth, life, loss, light and cavity.
DOI: 10.7284/102743
title: INS (Inertial Navigation System) data collected during research cruise MGL1113 using a Kongsberg Seapath 200 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7482692.v1
title: Survival evaluation of bacterial indicators and viruses during thermal drying of sewage sludge in a greenhouse

description: ABSTRACT The aim of the study was to evaluate the thermic drying of sewage sludge in agricultural greenhouse, in order to inactivate bacterial and virus, and to evaluate the behavior of somatic coliphages as indicator of these organisms. Each volume of excess sewage sludge discarded from UASB reactor, drained in a drying bed for 14 days and directed to thermic drying treatment in greenhouse corresponded to a batch (B) of sludge. Between February and November 2015, nine batches (L1 to L9) were treated for an average of 13 weeks. Most batches kept the pH between 5.0-6.0. After about 60 days of treatment, all batches showed humidity below 10%, except B3 and B4 (10-25%). The time required to reach 90% of total solids ranged between 40-50 (B1 to B7), 60 (B5, B6) and 85-100 (B2, B3 and B4) days. On average, levels of E. coli reduced to &lt;1x103 NMP/g TS after 50-60 days of treatment. The detection of somatic coliphages was low and eventual. Tests of inoculation showed rapid decay of virus (Sabin 1 strain poliovirus), but somatic coliphage Phi X174 was more persistent in the three temperatures evaluated (greenhouse, laboratory controlled 30 and 60°C). The thermic drying treatment of sewage sludge in agricultural greenhouse was presented as simple and efficient in obtaining granulated and dried product, and in reducing bacterial contamination levels given the parameter set as safe for agricultural use under the Brazilian law.
DOI: 10.6084/m9.figshare.7482698.v1
title: Resveratrol Attenuates LPS-induced Apoptosis via Inhibiting NF- κ B Activity in Chicken Peripheral Lymphocyte Cultures

description: ABSTRACT This study aims to investigate whether resveratrol (RES) could inhibit NF-κB activity and protect in vitro cultured chicken lymphocytes from apoptosis induced by continuous LPS stimulation. Blood lymphocytes of chickens were collected and cultured. LPS was added to the samples of treatment group, while the same volume of normal saline (NS) was supplemented to those of the control group. In the treatment groups, four different concentrations of RES (0, 20, 40, and 80 µg/ml) were admixed. Then, the following indicators were tested: lymphocyte apoptotic rate, lymphocyte viability, NF-κB activity, TNF-a level, reactive oxygen species (ROS), and expression of apoptosis-related proteins (Fas/FasL and Caspase-8). The results indicated that the application of different concentrations of RES could significantly increase the viability of lymphocytes and decrease the apoptotic rate (p&lt;0.05) after continuous stimulations by LPS. The activity of NF-κB levels of TNF-a and ROS in the RES treatment subgroups was significantly decreased in comparison with that in the RES-blank subgroup (p&lt;0.05). The cells in the treatment group that had been treated with 20, 40 and 80 μl/ml of RES exhibited a significantly lower Fas and Caspase-8 expression level than that in the RES-blank subgroup (p&lt;0.05). These findings revealed that RES could substantially diminish the concentrations of ROS and TNF-a, down-regulate NF-κB activity, decrease the expression of Fas and Caspase-8, stimulate the activity of peripheral lymphocytes, and lower the rate of their apoptosis, induced by continuous LPS stimulation in chicken lymphocyte cultures.
DOI: 10.6084/m9.figshare.7482689
title: The Effect of Cyclic Heat Stress Applied to Different Broiler Chicken Brooding Stages on Animal Performance and Carcass Yield

description: ABSTRACT The objective of this study was to evaluate the performance and carcass yield of broilers submitted to heat stress during different rearing stages. A total of 840 one-day-old CobbAvian48TM male broilers were housed in an experimental house equipped with conventional ventilation system and foggers. Birds were distributed according to a completely randomized design into four treatments, with six replicates of 35 birds each. The treatments consisted of: T1 (control) - birds reared under natural temperature and relative humidity conditions from 1 to 42 days of age; T2 - birds submitted to heat stress (HS) from 16 to 21 days of age; T3 - birds submitted to HS from 22 to 42 days of age; and T4 - birds submitted to HS from 16 to 42 days of age. Birds were submitted to heat stress daily for one hour (12:00-13:00h).On day 42, performance data were determined. Six birds per replicate were selected and sacrificed to obtain carcass, parts, and giblet weights and yields. Performance parameters were not influenced by the treatments. Broilers submitted to 1-h cyclic heat between 16 and 42 days of age presented lower deboned breast weight compared with those maintained in natural temperature and relative humidity conditions. It was concluded that the performance of broilers submitted to short cyclic heat periods is not impaired.
DOI: 10.6084/m9.figshare.7482695.v1
title: The career advancement challenge faced by female executives in Peruvian organisations

description: Abstract The percentage of women in Peru's workforce overall has been growing at a steady rate, but there are still few women are seen in executive positions. This qualitative study describes the challenges in women's professional career advancement in a country in which the cultural pattern of business is dominated by men and where specific roles have been assigned to women. In-depth interviews were conducted with 24 professionals, 11 women and 13 men. The interviews were analysed using qualitative data analysis software. The results show that family responsibilities were the main challenge for female executives' career advancement. Moreover, family responsibilities were linked to other challenges, such as physical mobility, educational attainment, professional experience and informal networks. Family responsibilities can be considered a transcultural challenge to the career advancement of female executives. Finally, the study shows that a country's culture is an important challenge to career advancement, as in the Peruvian context where women are still considered responsible for attending to the family and are assigned a fundamental role in raising the children.
DOI: 10.7284/102740
title: Meteorological Station data collected during research cruise MGL1113 using a RM Young 26700 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7482683
title: Methylation of p16 ink4a promoter is independent of human papillomavirus DNA physical state: a comparison between cervical pre-neoplastic and neoplastic samples

description: BACKGROUND Epigenetic modifications in host cells, like p16 ink4a methylation, have been considered as putative complementary mechanisms for cancer development. Because only a small proportion of infected women develop cervical cancer, other factors might be involved in carcinogenesis, either independently or in association with high-risk human papillomavirus (HR-HPV) infections, including epigenetic factors. OBJECTIVES We hypothesised that p16 ink4a methylation might have a role in cancer development driven by HPV16, mainly in the presence of intact E1/E2 genes. Thus, our objectives were to assess the status of p16 ink4a methylation and the HPV16 E1/E2 integrity in samples in different stages of cervical diseases. METHODS Presence of HPV16 was determined by E6 type-specific polymerase chain reaction (PCR). Methylation status of the p16 ink4a promoter was assessed by methylation-specific PCR in 87 cervical specimens comprising 29 low-grade (LSIL), 41 high-grade (HSIL) lesions, and 17 cervical cancers (CC). Characterisation of E1 and E2 disruption (as an indirect indicator of the presence of episomal viral DNA) was performed by PCR amplifications. FINDINGS We observed a significantly increased trend (nptrend = 0.0320) in the proportion of methylated p16 ink4a in cervical samples during cancer development. Concomitant E1 and E2 disruptions were the most frequent pattern found in all groups: CC (76%), HSIL (54%), and LSIL (73%). No statistically significant differences between p16 ink4a methylation and E1/E2 integrity, in histological groups, was observed. MAIN CONCLUSIONS There was an increase in methylation of the p16 ink4a promoter from pre-neoplastic lesions to cancer. Additionally, a high frequency of E1/E2 disruptions in LSIL/HSIL suggested that viral DNA integration was an early event in cervical disease. Moreover, the methylation status was apparently independent of HPV16 integrity.
DOI: 10.6084/m9.figshare.7482686
title: Clinical Outcomes and Cost-Effectiveness Analysis of FFR Compared with Angiography in Multivessel Disease Patient

description: Abstract Background: In multivessel disease patients with moderate stenosis, fractional flow reserve (FFR) allows the analysis of the lesions and guides treatment, and could contribute to the cost-effectiveness (CE) of non-pharmacological stents (NPS). Objectives: To evaluate CE and clinical impact of FFR-guided versus angiography-guided angioplasty (ANGIO) in multivessel patients using NPS. Methods: Multivessel disease patients were prospectively randomized to FFR or ANGIO groups during a 5 year-period and followed for &lt; 12 months. Outcomes measures were major adverse cardiac events (MACE), restenosis and CE. Results: We studied 69 patients, 47 (68.1%) men, aged 62.0 ± 9.0 years, 34 (49.2%) in FFR group and 53 (50.7%) in ANGIO group, with stable angina or acute coronary syndrome. In FFR, there were 26 patients with biarterial disease (76.5%) and 8 (23.5%) with triarterial disease, and in ANGIO, 24 (68.6%) with biarterial and 11 (31.4%) with triarterial disease. Twelve MACEs were observed - 3 deaths: 2 (5.8%) in FFR and 1 (2.8%) in ANGIO, 9 (13.0%) angina: 4(11.7%) in FFR and 5(14.2%) in ANGIO, 6 restenosis: 2(5.8%) in FFR and 4 (11.4%) in ANGIO. Angiography detected 87(53.0%) lesions in FFR, 39(23.7%) with PCI and 48(29.3%) with medical treatment; and 77 (47.0%) lesions in ANGIO, all treated with angioplasty. Thirty-nine (33.3%) stents were registered in FFR (0.45 ± 0.50 stents/lesion) and 78 (1.05 ± 0.22 stents/lesion) in ANGIO (p = 0.0001), 51.4% greater in ANGIO than FFR. CE analysis revealed a cost of BRL 5,045.97 BRL 5,430.60 in ANGIO and FFR, respectively. The difference of effectiveness was of 1.82%. Conclusion: FFR reduced the number of lesions treated and stents, and the need for target-lesion revascularization, with a CE comparable with that of angiography.
DOI: 10.6084/m9.figshare.7482680
title: Sizing optimization in Solar Photovoltaic Water Pumping System: model validation in a pilot unit located in the rural community of Rio Belo, Orleans/SC, Brazil

description: ABSTRACT The management of water and energy resources are the main challenges that contemporary society faces nowadays. Water and energy are fundamental factors for socio-economic development of the communities, especially those in remote locations, and in this case the lack of energy is the most worrying aspect. Solar photovoltaic pumping systems have advantages for insertion in isolated communities, such as easy installation and low maintenance during the life cycle, but inappropriate sizing can create unnecessary costs or affect the water supply. Simulating performance favors the system’s sizing and allows achieving technical and economic optimization of these technology projects. This study proposes a mathematical model of simulation, use of the Generalized Reduced Gradient (GRG), solar radiation regional data, and water consumption, wells’ productivity and pumping system capacity to obtain this optimization. The model was validated in a pilot system, located in a rural community in the city of Orleans, State of Santa Catarina, Brazil. The place has one of the lowest average annual radiation in the country, approximately 4.5 kWh / m2.day. The simulation performed in the natural pilot system conditions presented results very close to the monitored behavior, thus demonstrating its efficiency. Using this tool assists in the decision-making process in designing projects and benefits the communities with access to water and energy, bringing benefits in all spheres: social, environmental and economic.