cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6075/j0cc10ts
title: CIL:40260, Mus musculus, pyramidal cell. In Cell Image Library

description: Perfused with 4% PFA, 0.1% glut. Postfixed 1 hour in same fix on ice. Left hemisphere cut into 2 mm thick coronal slabs. Tissue placed into 2.3% potassium dichromate / 0.4% OsO4 for 3 days at room temp. Tissue blotted with filter paper and then placed in 0.7% silver nitrate in dark for 3 days. Tissue was dehydrated, infiltrated and embedded in Durcupan in Beem capsules.

Serial section images were gathered on an FEI Quanta 200 FEG SEM with Gatan 3 View microscope, magnification, 600.0 X, accelerating voltage, 2.0 kV.
description: Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: serial block face SEM (SBFSEM)
Parameter imaged: back-scattered_electron imaging
Data qualification: Processed
description: Maximum intensity projection of Golgi impregnated pyramidal neurons in the neocortex of an adult mouse, imaged via serial block-face scanning electron microscopy. Contrast is reversed so that Golgi stain appears bright against the unstained background. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
DOI: 10.6075/j0zg6rkz
title: CIL:39227, Psophus stridulus, spermatocyte. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: film
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Data qualification: Processed
description: Time lapse movie of meiosis in spermatocytes of the Locust Psophus stridulus showing telophase of meiosis I, cytokinesis, interphase, and prophase of meiosis II.
 This is a historic movie made in the early 40s by Kurt Michel of the Zeiss company in Jena using Zernike's newly invented phase contrast microscope. 
 Despite its early date, the movie and the others in the group remain some of the clearest examples of chromosome behavior during meiosis.
description: The movie was originally recorded on 35mm film, and later transferred to CD format. The text at the beginning of the movie translates approximately as follows:

Meiosis division I, telophase and meiosis II, prophase.
(Interpase ca. 6-7 hr)

-----------------------
See also:
Michel, K. (1941): Die Darstellung von Chromosomen mittels des Phasenkontrastverfahrens. Die Naturwissenschaften 29, 61-62 
Michel, K. (1958): Die Reifeteilungen (Meiose) bei der Spermatogenese der Schnarrheuschrecke (Psophus stridulus l.) Institut für den Wissenschaftlichen Film, Göttingen
DOI: 10.6075/j0xd118b
title: CIL: 52699, Homo sapiens, High-grade serious overian cancer. In Cell Image Library

description: One z layer of a z-stack at the given tile position
description: Imaging mode: Fluorescence in situ hybridization (FISH)
Visualization methods: Nikon Eclipse fluorescence inverted microscope equipped with a charge-coupled device camera (Andor Neo sCMOS), using filter sets for DAPI/ YGFP/TRITC/CY GFP with an objective lens (Plan Apo VC 100x, Nikon). Captured with 100x magnification of the objective and a pixel size of 0.07 microns
description: Tile x=4, y=2, z=2 from PS11_10021_2B_Myc_Terc
DOI: 10.6075/j0f18xfv
title: CIL:13707, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; tetramethylrhodamine, ethyl ester (TMRE)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: YFP-Parkin (green) localizes to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl) and treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA for 192 hours, treated with CCCP (10µM) for 1 hour and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig1f, middle panels and is a control for other images in Fig1f in J Cell Biol. 191: 933-942, 2010. Images in Fig1f include CIL#s 13707, 13708, 13709.
DOI: 10.6075/j0gm860h
title: CIL:13701, Chlorocebus aethiops. In Cell Image Library

description: Preparation: crosslinking-fixative fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 594
Data qualification: Raw
description: This is one of a group of four images in Figure S5 in Apaja et al., JCB 2010, that support the conclusion that the WT vasopressin type2 receptor (V2R) and the V2R W164S mutant (that causes nephrogenic diabetes insipidus) have different post-endocytic targeting. The sorting of endocytosed myc-tagged WT and W164S V2R was determined by indirect immunolabeling of internalized receptors in transiently transfected COS7 cells. The WT and temperature (26 degrees C) rescued mutant receptors were labelled with anti-myc Ab in live cells (1h) and then chased in Ab-free medium (1h, 37 degrees C) before indirect immunolabeling. Endosomes were labeled with 10 µg/ml Alexa Fluor 594–transferrin uptake for 1 h at 37°C. Fluorescence micrographs were obtained by a microscope (LSM510 or LSM710; Carl Zeiss, Inc.) equipped with a Plan-Apochromat 63×/NA 1.4 objective in multitrack mode. This single optical section shows that the internalized WT V2R is found in endosomes. Companion images show that the mutant V2R, however, co-localizes with lysosomes, but not endosomes.
DOI: 10.6075/j0nz86mk
title: CIL:2636, Caenorhabditis elegans. In Cell Image Library

description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 12 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
DOI: 10.6075/j0m32thg
title: CIL:13711, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; tetramethylrhodamine, ethyl ester (TMRE)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: YFP-Parkin (green) does not translocate to mitochondria (red) in HeLa cells. HeLa cells stably expressing YFP-Parkin were treated with DMSO for 3 hrs and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig2d, top panels and is a control for other images in Fig2d in J Cell Biol. 191: 933-942, 2010. Images in Fig2d include CIL#s 13710, 13711, 13712.
DOI: 10.6075/j0qj7g1c
title: CIL:13710, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; tetramethylrhodamine, ethyl ester (TMRE)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are treated with the proteosome inhibitor, MG132. HeLa cells stably expressing YFP-Parkin were treated with MG132 (10µM) for 3 hours and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig2d, bottom panels in J Cell Biol. 191: 933-942, 2010. Images in Fig2d include CIL#s 13710, 13711, 13712.
DOI: 10.6075/j0sn07p0
title: CIL:13709, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; tetramethylrhodamine, ethyl ester (TMRE)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are transfected with PARL (presenilin-associated rhomboid-like protein) siRNA. HeLa cells stably expressing YFP-Parkin were transfected with PARL siRNA for 192 hours, treated with DMSO for 1 hour and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig1f, bottom panels in J Cell Biol. 191: 933-942, 2010. Images in Fig1f include CIL#s 13707, 13708, 13709.
DOI: 10.6075/j0b56jgf
title: CIL: 54233, Homo sapiens, HeLa. In Cell Image Library

description: Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.
description: Visualization methods: confocal microscopy
description: TKL family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.
DOI: 10.6075/j02z14mt
title: CIL:2591, Caenorhabditis elegans. In Cell Image Library

description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 10 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
DOI: 10.6075/j0pk0dw4
title: CIL:13716, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; CMX rosamine (Mitotracker Red)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Wild-type PINK1-YFP (green) is rapidly degraded in HeLa cells. HeLa cells expressing PINK1-YFP were stained with 10nM MitoTracker Red (red) for 30 min to see mitochondrial morphology and then treated with DMSO for 3 hours. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). This image corresponds to Fig 3d, WT/DMSO top panels and is a control for other images in Fig 3d in J Cell Biol. 191: 933-942, 2010. Images in Fig3d include CIL#s 13713, 13714, 13715, 13716.
DOI: 10.15468/dl.9dhduv
title: Occurrence Download

description: A dataset containing 46405 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Acer pictum, Betula platyphylla, Juglans mandshurica, Populus davidiana, Fraxinus mandshurica, Quercus mongolica, Ulmus davidiana, Castanopsis fissa, Machilus chinensis, Schima superba, Triadica cochinchinensis, Cryptocarya chinensis, Gironniera subaequalis, Psychotria asiatica, Pterospermum heterophyllum)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Human Observation)",
 "OccurrenceStatus is Present",
 "EventDate is greater than or equal to 1991-01-01",
 "EventDate is less than or equal to 2025-10-31",
 {
 "or" : [
 "CoordinateUncertaintyInMeters is less than or equal to 10000",
 "CoordinateUncertaintyInMeters is null"
 ]
 }
 ]
}

 The dataset includes 46405 records from 254 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0009431-251025141854904/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.6075/j04m93k5
title: CIL:2547, Caenorhabditis elegans. In Cell Image Library

description: The purpose of the dataset C. elegans terminal bulb aging is to deduce the age of the nemathode based on images of the pharynx terminal bulb (part of the eating apparatus). Images of C. elegans were taken at different chronological ages. This image is part of the day 10 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.
description: Terminal Bulb is a dataset of DIC (differential interference contrast) 20x microscopy images of the pharynx terminal bulb of C. elegans at different ages (0, 2, 4, 6, 8, 10, 12 days). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1587, 1694, 1905, 2213, 2622, 2544, and 2606, respectively.
description: Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
DOI: 10.6075/j0j38r85
title: CIL:13713, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library

description: Upon mitochondrial uncoupling, wild-type PINK1-YFP (green) is localized in mitochondria (red) in HeLa cells. HeLa cells expressing WT PINK1-YFP were stained with 10nM MitoTracker Red (red) for 30 min to see mitochondrial morphology prior to treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) (10µM) for 3 hours. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). This image corresponds to Fig 3d, WT/CCCP top panels in J Cell Biol. 191: 933-942, 2010. Images in Fig3d include CIL#s 13713, 13714, 13715, 13716.
description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; CMX rosamine (Mitotracker Red)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j08c9vdv
title: CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

description: Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
description: Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. Magnification 21,000 X. See Ladinsky and Howell (2007) for more information on methods used.