cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/x22z4-0ba23
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC106
description: <strong>Tilt Series Date:</strong> 2014-07-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.</p>
  ycw2014-07-07-11_slicer7441.jpg: Flat vesicle in Myxococcus xanthus delta-tsaP ycw2014-07-07-11_slicer7444.jpg: Filament bundle in Myxococcus xanthus delta-tsaP
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-11/rawdata/</p>20140707_YWC106_10013.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-11/rawdata/20140707_YWC106_10013.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140707_YWC106_10013_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-11/3dimage_44398/20140707_YWC106_10013_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/xn6jk-7fs72
title: H. pylori 26695

description: Grid box YWC050Incubated with 20 nm gold/DNA conjugate for 80 min
description: <strong>Tilt Series Date:</strong> 2013-11-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 3 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-11-24-3_slicer9937.jpg: Helicobacter pylori .  Ribosome exclusion zone REZ .  chemoreceptor
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-24-3/rawdata/</p>20131124_YWC050_10003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-24-3/rawdata/20131124_YWC050_10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131124_YWC050_10003_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-24-3/3dimage_34970/20131124_YWC050_10003_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/pn83w-a2a58
title: M. xanthus (Dk1622)

description: Imaged for Lotte in search of T6SS. This is the WT strain that they work with.
description: <strong>Tilt Series Date:</strong> 2012-08-17</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 23000x. </p>
<strong>Microscope:</strong> UCLA Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Lotte Soregard-Anderson</p>
  mst2012-08-17-41_slicer5331.jpg: nested vesicle .  [2014-08-06 13:39:24] Myxococcus Xanthus mst2012-08-17-41_slicer5333.jpg: nested vesicle .  [2014-08-06 13:41:19] Myxococcus Xanthus mst2012-08-17-41_slicer5336.jpg: nested vesicle .  [2014-08-06 13:41:23] Myxococcus Xanthus mst2012-08-17-41_slicer5344.jpg: flat vesicle mst2012-08-17-41_slicer5347.jpg: nested vesicle .  [2014-08-06 13:41:28] Myxococcus Xanthus mst2012-08-17-41_slicer5349.jpg: external vesicle mst2012-08-17-41_slicer5350.jpg: external vesicle mst2012-08-17-41_slicer5359.jpg: unknown filament
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2012-08-17-41/rawdata/</p>081712_2_DK1622_5.mrc,  Tilt Series (Pixel Size 1.11 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2012-08-17-41/rawdata/081712_2_DK1622_5.mrc"
        > <i class="download icon"></i> Download </a></p>

        081712_2_DK1622_5_part129_45.rec,  Reconstruction (Pixel Size 2.22 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2012-08-17-41/3dimage_43462/081712_2_DK1622_5_part129_45.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6ar8v-1bb95
title: SA5718_cryoPALM(20130220)

description: Cell with VipA-PA-GFP/ΔvipA.First imaged by cryoPALM.Grid dried up inside Polara column overtime.
description: <strong>Tilt Series Date:</strong> 2013-06-08</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-61.0°, 53.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Lotte Søgaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> (1) Cells grown 48 hr in CTT medium at 30 degrees without antibiotics to OD600~0.8. (2) 250 ul CTT with 20 % Dextran + 1 ul Invitrogen 200 nm orange fluosphere. (3) take 10 ul of the mixture and add into gold fiducial pellet. (4) add 10 ul cells prior to plunge freezing</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-08-7/rawdata/</p>20130608_20130220_SA5718_cryoPALM_10007.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.3 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-08-7/rawdata/20130608_20130220_SA5718_cryoPALM_10007.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130608_20130220_SA5718_cryoPALM_10007_full.rec,  Reconstruction (Pixel Size 1.92 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-06-08-7/3dimage_27332/20130608_20130220_SA5718_cryoPALM_10007_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/1qy2r-4rc89
title: Myxococcus xanthus delta-pilT

description: grid box YWC063
description: <strong>Tilt Series Date:</strong> 2013-12-21</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.</p>
  ycw2013-12-21-15_slicer8197.jpg: half filled vesicle, Myxococcus Xanthus
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-15/rawdata/</p>20131221_YWC063_10015.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-15/rawdata/20131221_YWC063_10015.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131221_YWC063_10015_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-12-21-15/3dimage_36368/20131221_YWC063_10015_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9q2nv-wd313
title: Helicobacter pylori 26695

description: Grid box YWC043
description: <strong>Tilt Series Date:</strong> 2013-10-25</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Helicobacter pylori</p>
<strong>Strain:</strong> 26695</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Cell prepared by Lee Rettberg.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200</p>
  ycw2013-10-25-89_slicer17909.jpg: cag T4SS wi fi particle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-89/rawdata/</p>20131025_Janelia_YWC043_20035.mrc,  Tilt Series (Pixel Size 0.42 nm), 2.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-89/rawdata/20131025_Janelia_YWC043_20035.mrc"
        > <i class="download icon"></i> Download </a></p>

        20131025_Janelia_YWC043_20035_full.rec,  Reconstruction (Pixel Size 1.68 nm), 688.9 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-25-89/3dimage_33564/20131025_Janelia_YWC043_20035_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/bkwhv-byj34
title: Sulfolobus solfac.P2+STIV

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection 2-4 hour post infection
description: <strong>Tilt Series Date:</strong> 2014-03-13</p>
<strong>Data Taken By:</strong> Rasika Ramdasi</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus </p>
<strong>Strain:</strong> P2</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF TOMO</p>
<strong>Collaborators and Roles:</strong> With Jamie Snyder at MSU, Montana.</p>
<strong>Sample Preparation:</strong> S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 2-4 hour Post infection, cells were concentrated ~20 fold by centrifugation.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-03-13-62/rawdata/</p>stiv4atlas20010.mrc,  Tilt Series (Pixel Size 0.63 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-03-13-62/rawdata/stiv4atlas20010.mrc"
        > <i class="download icon"></i> Download </a></p>

        stiv4atlas20010_full.rec,  Reconstruction (Pixel Size 2.52 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/rra2014-03-13-62/3dimage_40423/stiv4atlas20010_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.an3ue5
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Baileychromis centropomoides, Barboides gracilis, Barbopsis devecchii, Barbus hondeensis, Enteromius manicensis, Barbus kamaia, Enteromius motebensis, Barbus microbarbis, Labeobarbus microbarbis, Barbus microterolepis, Labeobarbus microterolepis, Barbus myersi, Enteromius radiatus, Barbus paucisquamatus, Labeobarbus paucisquamatus, Barbus tetraspilus, Barilius shariensis, Raiamas shariensis, Bathyaethiops breuseghemi, Bathyaethiops caudomaculatus, Bathyaethiops greeni, Bathybagrus grandis, Bathybagrus graueri, Bathybagrus platycephalus, Bathybagrus sianenna, Bathybagrus stappersii, Bathybagrus tetranema, Bathybates fasciatus, Bathybates ferox, Bathybates graueri, Bathybates hornii, Bathybates leo, Bathybates minor, Bathybates vittatus, Bathyclarias atribranchus, Bathyclarias euryodon, Bathyclarias filicibarbis, Bathyclarias foveolatus, Bathyclarias longibarbis, Bathyclarias nyasensis, Bathyclarias rotundifrons, Bathyclarias worthingtoni, Bathygobius casamancus, Bathygobius fuscus, Bathygobius petrophilus, Bathygobius samberanoensis, Bathygobius soporator, Bedotia albomarginata, Bedotia alveyi, Bedotia geayi, Bedotia leucopteron, Bedotia longianalis, Bedotia madagascariensis, Bedotia marojejy, Bedotia masoala, Bedotia tricolor, Belonoglanis brieni, Belonoglanis tenuis, Belonophago hutsebouti, Belonophago tinanti, Benitochromis batesii, Benitochromis conjunctus, Benitochromis finleyi, Benitochromis nigrodorsalis, Benitochromis riomuniensis, Benitochromis ufermanni, Benthochromis melanoides, Benthochromis tricoti, Boleophthalmus boddarti, Bostrychus africanus, Boulengerochromis microlepis, Boulengeromyrus knoepffleri, Brachyalestes bimaculatus, Brachyalestes carolinae, Brachyalestes comptus, Brachyalestes fwaensis, Brachyalestes imberi, Brycinus imberi, Brachyalestes jacksonii, Brachyalestes kingsleyae, Brachyalestes nurse, Brachypetersius altus, Brachypetersius cadwaladeri, Brachypetersius gabonensis, Brachypetersius huloti, Brachypetersius pseudonummifer, Brevimyrus niger, Brienomyrus brachyistius)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "BasisOfRecord is one of (Specimen, Living Specimen, Observation, Human Observation, Machine Observation, Material sample, Literature Occurrence, Material citation, Occurrence evidence)",
 "Geometry POLYGON ((-17.0425 19.3358,-16.8618 19.0648,-16.8618 20.0599,-16.9237 20.0599,-17.0425 19.3358))"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0006113-250827131500795/datasets/export for details.
DOI: 10.22002/a2f7a-6gc33
title: Rhodobacter1 558 lysed/PVP

description: Rhodobacter lysed n the presence of the crowding agent 10% Polyvinylpyrrolidone (PVP)
description: <strong>Tilt Series Date:</strong> 2013-10-07</p>
<strong>Data Taken By:</strong> Ariane Briegel</p>
<strong>Species / Specimen:</strong> Rhodobacter sphaeroides</p>
<strong>Strain:</strong> ATCC 17023</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -10.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> SIRT</p>
<strong>Collaborators and Roles:</strong> Judy Armitage</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Protocol:-     grew 5mL cultures of 1558 (with kanamycin and IPTG) to log phase (OD600 between 0.12-0.15)-     spun down 10' at 5000 xg-     resuspended pellets in 2mL appropriate Buffer 1-     Added 200µL fresh lysozme (50 mg/mL in 10mM Tris-HCl, pH 8.0)-     Incubated 15' at RT-     Added 11.2µL 1M MgCl2, 1.3µL 1M CaCl2, 2.2µL DNase I-     Incubated 30' at 37C-     Spun down 10' at 5000 xg-     resuspended PVP in 80µL appropriate Buffer 2Buffer 1:Crowding agent1 g sucroseQ.S. 5mL 25mM Tris-HCl, pH 7.0Buffer 2:Crowding agentQ.S. 5mL 25mM Tris-HCl, pH 7.0</p>
description: Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-22/rawdata/</p>Rhodopvp27k0024.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-22/rawdata/Rhodopvp27k0024.mrc"
        > <i class="download icon"></i> Download </a></p>

        Rhodopvp27k0024SIRTrot.rec,  Reconstruction (Pixel Size 0.0 nm), 11.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2013-10-07-22/3dimage_46410/Rhodopvp27k0024SIRTrot.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6p091-a9376
title: S. pombe (Cdc25-22)

description: Cryosections of S. pombe (Cdc25-22) synchronized for actomyosin ring formation.
description: <strong>Tilt Series Date:</strong> 2013-09-24</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Schizosaccharomyces pombe</p>
<strong>Strain:</strong> cdc25-22 rlc1-3GFP</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Mohan Balasubramanian and Mithilesh Mishra</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown to OD = 0.2 and them temperature shifted to the nonpermissive temp of 36C for 4 hours. Then cells were shifted to the permissive temp of 25C and monitored by fLM for ring formation (between 30-60 min). ~90 are synchronized.</p>
<strong>Sample Preparation:</strong> After synchronization cells were pelleted, mixed to include ~ 20% dextran and high pressure frozen. They were then cryosectioned at 150-200 nm.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-24-2/3dimage_44106/</p>092413_pombe0003.rec,  Reconstruction (Pixel Size 2.464 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-24-2/3dimage_44106/092413_pombe0003.rec"
        > <i class="download icon"></i> Download </a></p>

        092413_pombe0003.mrc,  Tilt Series (Pixel Size 0.616 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-09-24-2/rawdata/092413_pombe0003.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tgk3p-rk922
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-03-07</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-03-07-22_slicer7487.jpg: T6SS in Myxococcus xanthus PilO-sfGFP ycw2014-03-07-22_slicer7488.jpg: unknown tube in Myxococcus xanthus PilO-sfGFP
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-03-07-22/rawdata/</p>20140306_YWC092_10025.mrc,  Tilt Series (Pixel Size 4.2 nm), 3.6 GB  
        
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        20140306_YWC092_10025_part131_5.rec,  Reconstruction (Pixel Size 16.8 nm), 688.9 MB  
        
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