cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.11314034.v1
title: Monitoring of stem cells from adipose tissue injected via retrobulbar next to previously injured optic nerve of rabbits

description: Abstract Obtective: To verify the presence of mesenchymal stem cells (MSC) in the area close to the optic nerve of previously injured with absolute alcohol. Methods: Twelve New Zealand breed rabbits were divided into two groups, and after sedation, each eye of the animal received a retrobulbar injection of 1 ml of absolute ethanol in one eye, and 1 ml of physiological solution 0.9 % (PS) in the contralateral eye. After 15 days all eyes of animals belonging to group A, received via retrobulbar a solution containing MSCs from human adipose tissue (AT) and previously marked with Qdots, while all eyes of animals from group B received solution containing PBS. Results: The presence of MSC was observed in 100% of the eyes of the animals of group A and the more central areas near and into the optic nerve. Conclusion: The results suggest that the appointment of MSC with Qdots allowed their follow-up applied in the region and in the inner areas of the optic nerve. The MSC permanence after 15 days of application around the optic nerve suggests the feasibility and possible involvement of the same during the damaged tissue regeneration process. Under the conditions of this study, the route of retrobulbar application and the presence of the stem cells to the central areas of the optic nerves in animals of group A, suggests that this might be an effective approach for MSCs in regeneration process of optic neuropathies.
DOI: 10.6084/m9.figshare.11314019
title: Phytogenic as feed additive for suckling dairy calves’ has a beneficial effect on animal health and performance

description: Abstract: The aim of this study was to evaluate whether the use phytogenic as feed additive of Holstein calves could exert beneficial effects on animal health and performance. Thirty newborn calves were randomly divided into two groups (control and treated), and the treated consisted of 10 g of the phytogenic product diluted in milk once a day for 30 days. Blood and fecal samples were collected, and fecal score was performed daily in order to determine the occurrence of diarrhea. Treated calves showed higher hematocrit on days 45 and 60 of life. Serum levels of urea, total proteins, globulins and glucose were lower in the treated group on day 60 of life, while triglycerides were lower on days 15 and 60 of life. Serum glutathione S-transferase activity was higher in treated animals on day 60 of life. Fecal bacterial count was lower in treated animals on days 30 and 60 of life. The weight gain was higher in treated animals on days 60 and 210 of life. Based on these results, it is possible to conclude that the addition of phytogenic as feed additive for calves was able to minimize fecal bacterial infections and to increase body weight gain.
DOI: 10.6084/m9.figshare.11314022.v1
title: Parasitological and histological aspects of Holochilus sciureus naturally infected by Schistosoma mansoni

description: Abstract Schistosomiasis is a neglected disease that affects millions of people around the world, being common in the state of Maranhão. A total of 225 rodents of the Holochilus sciureus species from the Western Lowland Maranhão were studied, of which 144 animals (64%) exhibited Schistosoma eggs in their feces samples. Macroscopic lesions characterized as well-defined whitish areas on the liver and spleen surfaces were observed. Histopathological examination revealed multifocal granulomas in the esophagus, liver, spleen, pancreas and duodenum, with structures compatible with Schistosoma mansoni eggs, as well as severe hepatic micro-vacuolar degeneration, multifocal and coalescent, with proliferation of random bile ducts and associated epithelial hyperplasia to areas of fibrosis. Adult forms of the parasite were observed in the blood vessels of the portal space. The lungs exhibited moderate and diffuse interstitial pneumonia with intralesional S. mansoni eggs. In the kidneys, hyaline cylinders were observed in the pelvis and diffuse hemorrhage. In conclusion, H. sciureus displays a pathological picture similar to human being. This rodent plays a role as sentinel in Baixada Maranhense.
DOI: 10.6084/m9.figshare.11314028.v1
title: SBEM and knowledge production for Mathematical Education

description: Abstract At the end of 1980s, with the creation of SBEM (Brazilian Society of Mathematical Education), the scientific space of Mathematical Education was expanded to new times, in which modern Mathematics declined and technological advances rose up, especially concerning the questioning of mathematics teachers’ professional knowledge. Within the scope of these questionings, along with the appreciation of the subjective aspects in teacher's training, there are concerns about the complexity involved in the characterization of professional knowledge and the difficulty of systematizing them in face of their transformation into institutionalized knowledge. Based on the assumption that professional knowledges are those that have undergone complex processes of objectification and systematization until they become institutionalized and integrate the formation of a certain socially recognized profession, we selected six seminal studies in an attempt to understand what knowledge was considered essential for the teacher to teach Mathematics nowadays. The results point out to the existence of a non-dispersed and fragmented formation, capable of expressing an appropriate professional sense for a new century, that puts in tune knowledge of the reference science with knowledge of educational sciences, Mathematics teaching knowledge with art and science and, suitable for teaching in an ever-changing world.
DOI: 10.6084/m9.figshare.11314013
title: The impact of cooperatives on Brazilian agricultural production: a spatial econometric analysis

description: Abstract Cooperatives are important links between producers and the market, responding directly or indirectly for a relevant portion of the agricultural Gross Domestic Product. There is a lack of studies that measure how much cooperatives influence production in the rural environment, considering Brazilian regional differences. Thus, the objective is to identify the influence of cooperatives in the agricultural production of Brazilian regions. Therefore, a production function considering cooperatives as one of its inputs was constructed, considering a spatial correction at the municipal level for Brazilian regions. The results show that there is spatial dependence on the data used, justifying the methodological approach used in this work. There was a positive effect of cooperatives on the Gross Value of Agricultural Production in the municipalities of the Southeast, Center-West and South, while the restrictive influence of the cooperative association in the North and Northeast was noted. Therefore, the expansion of cooperatives throughout the regions was not a homogeneous process, and there is still a long way to go in order to have higher levels of cooperation in the Brazilian countryside.
DOI: 10.6084/m9.figshare.11314016
title: Efficacy of afoxolaner in the flea control in experimentally infested cats

description: Abstract The aim of this study was to evaluate the efficacy of a single dose of oral afoxolaner in controlling fleas in cats. Fourteen cats were used. The cats were given identification numbers, housed individually, artificially infested with Ctenocephalides felis felis, and treated (or not) with afoxolaner. Were divided into a treatment group and a control group (n = 7/group), on the basis of the fleas count hours after an infestation applied on Day (one-by-one allocation after ordering by count). At the start of the experimental protocol (designated day 0), the treated group received afoxolaner in a single dose of 2.5 mg/kg and the control group animals received a placebo. All animals were infested with 100 C. felis felis fleas two days before day 0, as well as on days 5, 12, 19, 26, 33, 40, 47, 54, and 63, parasite loads being evaluated at 48 h after each infestation. The efficacy of afoxolaner was 100% on day 2 and remained above 98% until day 42, decreasing to 95.3% by day 63. The findings confirm that a single dose of oral afoxolaner was effective in controlling C. felis felis in cats, and there were no observed adverse events.
DOI: 10.6084/m9.figshare.11314019.v1
title: Phytogenic as feed additive for suckling dairy calves’ has a beneficial effect on animal health and performance

description: Abstract: The aim of this study was to evaluate whether the use phytogenic as feed additive of Holstein calves could exert beneficial effects on animal health and performance. Thirty newborn calves were randomly divided into two groups (control and treated), and the treated consisted of 10 g of the phytogenic product diluted in milk once a day for 30 days. Blood and fecal samples were collected, and fecal score was performed daily in order to determine the occurrence of diarrhea. Treated calves showed higher hematocrit on days 45 and 60 of life. Serum levels of urea, total proteins, globulins and glucose were lower in the treated group on day 60 of life, while triglycerides were lower on days 15 and 60 of life. Serum glutathione S-transferase activity was higher in treated animals on day 60 of life. Fecal bacterial count was lower in treated animals on days 30 and 60 of life. The weight gain was higher in treated animals on days 60 and 210 of life. Based on these results, it is possible to conclude that the addition of phytogenic as feed additive for calves was able to minimize fecal bacterial infections and to increase body weight gain.
DOI: 10.6084/m9.figshare.11314010
title: Predicting of biomass in Brazilian tropical dry forest: a statistical evaluation of generic equations

description: ABSTRACT Dry tropical forests are a key component in the global carbon cycle and their biomass estimates depend almost exclusively of fitted equations for multi-species or individual species data. Therefore, a systematic evaluation of statistical models through validation of estimates of aboveground biomass stocks is justifiable. In this study was analyzed the capacity of generic and specific equations obtained from different locations in Mexico and Brazil, to estimate aboveground biomass at multi-species levels and for four different species. Generic equations developed in Mexico and Brazil performed better in estimating tree biomass for multi-species data. For Poincianella bracteosa and Mimosa ophthalmocentra, only the Sampaio and Silva (2005) generic equation was the most recommended. These equations indicate lower tendency and lower bias, and biomass estimates for these equations are similar. For the species Mimosa tenuiflora, Aspidosperma pyrifolium and for the genus Croton the specific regional equations are more recommended, although the generic equation of Sampaio and Silva (2005) is not discarded for biomass estimates. Models considering gender, families, successional groups, climatic variables and wood specific gravity should be adjusted, tested and the resulting equations should be validated at both local and regional levels as well as on the scales of tropics with dry forest dominance.
DOI: 10.6084/m9.figshare.11314025.v1
title: Gastrointestinal helminths and related histopathological lesions in black-necked swans Cygnus melancoryphus from the Carlos Anwandter Nature Sanctuary, Southern Chile

description: Abstract Black-necked swans are distributed across South America and face conservation problems in Chile according to data of the State institution SAG. The aim of this study was to identify helminths and to assess associated tissue damage via histopathology. A total of 19,291 parasites were isolated from 21 examined birds; 17 species were identified, including nematodes, flukes, and tapeworms. Of these, 12 were new host records, 13 were reported for the first time in Chile, and 5 were new records for the Neotropical region. Further, the flukes Schistosomatidae gen. sp. and Echinostoma echinatum are of zoonotic concern. Regarding histopathology, an inflammatory response was found along the birds’ entire digestive tract. Nevertheless, it is difficult to declare that there is a clear association between such lesions and isolated parasites, as other noxa could be responsible as well. Although in some cases there was an evident association, such inflammatory responses and necrosis were minimal, as occurred with Capillaria, Retinometra, Catatropis, Echinostoma, and Schistosomatidae gen. sp. Nevertheless, Epomidiostomum vogelsangi caused granulomatous injuries, an important inflammatory response, and necrosis, but it always circumscribed to superficial layers of the gizzard. Conversely, Paramonostomum was not associated with an inflammatory response despite a high parasitic load.
DOI: 10.6084/m9.figshare.11314007
title: Molecular survey of flaviviruses and orthobunyaviruses in Amblyomma spp. ticks collected in Minas Gerais, Brazil

description: Abstract Due to anthropic environmental changes, vector-borne diseases are emerging worldwide. Ticks are known vectors of several pathogens of concern among humans and animals. In recent decades, several examples of tick-borne emerging viral diseases have been reported (Crimean Congo hemorrhagic fever virus, Powassan virus, encephalitis virus, heartland virus, severe fever with thrombocytopenia syndrome virus). Unfortunately, few studies addressing the presence of viruses in wild ticks have been carried out in South America. With the aim of detecting flaviviruses and orthobunyaviruses in ticks, we carried out molecular detection in wild ticks collected in the state of Minas Gerais, Brazil. No Flavivirus-positive ticks were detected; however, we detected activity of Orthobunyavirus in 8 Amblyomma tick specimens. One of those individuals was positive for Bunyamwera orthobunyavirus, which represents the first report of this virus among ticks in South America. Further studies related to the ecology of zoonotic diseases are needed to increase knowledge of this topic, including attempts at viral isolation, full genome sequencing and biological characterization. In this way, we will obtain a better picture of the real risk of ticks as a vector for viral diseases for humans and animals on our continent, where no tick-borne viral disease is known to occur.
DOI: 10.6084/m9.figshare.11314013.v1
title: The impact of cooperatives on Brazilian agricultural production: a spatial econometric analysis

description: Abstract Cooperatives are important links between producers and the market, responding directly or indirectly for a relevant portion of the agricultural Gross Domestic Product. There is a lack of studies that measure how much cooperatives influence production in the rural environment, considering Brazilian regional differences. Thus, the objective is to identify the influence of cooperatives in the agricultural production of Brazilian regions. Therefore, a production function considering cooperatives as one of its inputs was constructed, considering a spatial correction at the municipal level for Brazilian regions. The results show that there is spatial dependence on the data used, justifying the methodological approach used in this work. There was a positive effect of cooperatives on the Gross Value of Agricultural Production in the municipalities of the Southeast, Center-West and South, while the restrictive influence of the cooperative association in the North and Northeast was noted. Therefore, the expansion of cooperatives throughout the regions was not a homogeneous process, and there is still a long way to go in order to have higher levels of cooperation in the Brazilian countryside.
DOI: 10.6084/m9.figshare.11314016.v1
title: Efficacy of afoxolaner in the flea control in experimentally infested cats

description: Abstract The aim of this study was to evaluate the efficacy of a single dose of oral afoxolaner in controlling fleas in cats. Fourteen cats were used. The cats were given identification numbers, housed individually, artificially infested with Ctenocephalides felis felis, and treated (or not) with afoxolaner. Were divided into a treatment group and a control group (n = 7/group), on the basis of the fleas count hours after an infestation applied on Day (one-by-one allocation after ordering by count). At the start of the experimental protocol (designated day 0), the treated group received afoxolaner in a single dose of 2.5 mg/kg and the control group animals received a placebo. All animals were infested with 100 C. felis felis fleas two days before day 0, as well as on days 5, 12, 19, 26, 33, 40, 47, 54, and 63, parasite loads being evaluated at 48 h after each infestation. The efficacy of afoxolaner was 100% on day 2 and remained above 98% until day 42, decreasing to 95.3% by day 63. The findings confirm that a single dose of oral afoxolaner was effective in controlling C. felis felis in cats, and there were no observed adverse events.
DOI: 10.6084/m9.figshare.11314010.v1
title: Predicting of biomass in Brazilian tropical dry forest: a statistical evaluation of generic equations

description: ABSTRACT Dry tropical forests are a key component in the global carbon cycle and their biomass estimates depend almost exclusively of fitted equations for multi-species or individual species data. Therefore, a systematic evaluation of statistical models through validation of estimates of aboveground biomass stocks is justifiable. In this study was analyzed the capacity of generic and specific equations obtained from different locations in Mexico and Brazil, to estimate aboveground biomass at multi-species levels and for four different species. Generic equations developed in Mexico and Brazil performed better in estimating tree biomass for multi-species data. For Poincianella bracteosa and Mimosa ophthalmocentra, only the Sampaio and Silva (2005) generic equation was the most recommended. These equations indicate lower tendency and lower bias, and biomass estimates for these equations are similar. For the species Mimosa tenuiflora, Aspidosperma pyrifolium and for the genus Croton the specific regional equations are more recommended, although the generic equation of Sampaio and Silva (2005) is not discarded for biomass estimates. Models considering gender, families, successional groups, climatic variables and wood specific gravity should be adjusted, tested and the resulting equations should be validated at both local and regional levels as well as on the scales of tropics with dry forest dominance.
DOI: 10.6084/m9.figshare.11313998
title: There is always something to teach, there is always something to learn: how and why should we teach mathematics to senior people?

description: Abstract This paper is related to the development of educative actions with senior people. More specifically, it refers to the Mathematical Education promoted in the space of university extension activities. It is considered that the engagement of senior people in educational environments can contribute to the maintenance and development of cognitive aspects, which are fundamental to their autonomy. In addition, participation in these environments can favor changes in their physical, social, and psychological aspects, enhancing their self-esteem. In this paper, we discuss results of a research which aimed at understanding possible ways to promote such educational actions and to providing elements to a reflection about its contributions to senior people. Data production occurred within the project Conversation about Mathematics with Senior People. Data were collect through interviews, photos, and notes in a research notebook. Data analysis reveals an active engagement of the women and men in the project as well as provides some indication about contributions to the senior people. About their engagement, we considered: a) their interest in discussing the subjects during the meetings; b) their persistence to perform mathematics tasks on their own; c) their argumentation about the ideas showed; and d) their movement of sharing the activities with other people who were not participating in the project. In relation to contribution to senior people, we considered manifestations that expressed: a) improvement of cognitive aspects; b) opportunity for social interaction; and c) the possibility of learning new subjects related to Mathematics.
DOI: 10.6084/m9.figshare.11314001
title: Diosmin induces caspase-dependent apoptosis in human glioblastoma cells

description: Abstract: Diosmin is a flavone glycoside clinically used as the main component of Daflon for the treatment of venous diseases. Several studies demonstrated that this natural compound can induce apoptosis in different tumors. However, isolated diosmin has not been studied regarding its effects on glioblastoma so far. Since glioblastoma is a highly lethal and fast-growing brain tumor, new therapeutic strategies are urgently needed. Herein, we evaluated the role of this flavonoid against glioblastoma cells using in vitro assays. Diosmin significantly reduced the viability of GBM95, GBM02, and U87MG glioblastoma cells, but not of healthy human astrocytes, as verified by MTT assay. Vimentin immunostaining showed that diosmin induced morphological changes in GBM95 and GBM02 cells, making them smaller and more polygonal. Diosmin did not inhibit GBM95 and GBM02 cell proliferation, but it caused DNA fragmentation, as verified by the TUNEL assay, and increased cleaved caspase-3 expression in these cells. In summary, diosmin is able to induce caspase-dependent apoptosis specifically in tumor cells and, therefore, could be considered a promising therapeutic compound against glioblastoma.
DOI: 10.6084/m9.figshare.11314007.v1
title: Molecular survey of flaviviruses and orthobunyaviruses in Amblyomma spp. ticks collected in Minas Gerais, Brazil

description: Abstract Due to anthropic environmental changes, vector-borne diseases are emerging worldwide. Ticks are known vectors of several pathogens of concern among humans and animals. In recent decades, several examples of tick-borne emerging viral diseases have been reported (Crimean Congo hemorrhagic fever virus, Powassan virus, encephalitis virus, heartland virus, severe fever with thrombocytopenia syndrome virus). Unfortunately, few studies addressing the presence of viruses in wild ticks have been carried out in South America. With the aim of detecting flaviviruses and orthobunyaviruses in ticks, we carried out molecular detection in wild ticks collected in the state of Minas Gerais, Brazil. No Flavivirus-positive ticks were detected; however, we detected activity of Orthobunyavirus in 8 Amblyomma tick specimens. One of those individuals was positive for Bunyamwera orthobunyavirus, which represents the first report of this virus among ticks in South America. Further studies related to the ecology of zoonotic diseases are needed to increase knowledge of this topic, including attempts at viral isolation, full genome sequencing and biological characterization. In this way, we will obtain a better picture of the real risk of ticks as a vector for viral diseases for humans and animals on our continent, where no tick-borne viral disease is known to occur.