DOI: 10.5061/dryad.sp418
title: Data from: Oceanographic currents and local ecological knowledge indicate, and genetics does not refute, a contemporary pattern of larval dispersal for the ornate spiny lobster, Panulirus ornatus in the South-East Asian archipelago

description: Here we utilize a combination of genetic data, oceanographic data, and
 local ecological knowledge to assess connectivity patterns of the ornate
 spiny lobster Panulirus ornatus (Fabricius, 1798) in the South-East Asian
 archipelago from Vietnam to Australia. Partial mitochondrial DNA control
 region and 10 polymorphic microsatellites did not detect genetic structure
 of 216 wild P. ornatus samples from Australia, Indonesia and Vietnam.
 Analyses show no evidence for genetic differentiation among populations
 (mtDNA control region sequences ΦST = -0.008; microsatellite loci FST =
 0.003). A lack of evidence for regional or localized mtDNA haplotype
 clusters, or geographic clusters of microsatellite genotypes, reveals a
 pattern of high gene flow in P. ornatus throughout the South-East Asian
 Archipelago. This lack of genetic structure may be due to the
 oceanography-driven connectivity of the pelagic lobster larvae between
 spawning grounds in Papua New Guinea, the Philippines and, possibly,
 Indonesia. The connectivity cycle necessitates three generations. The lack
 of genetic structure of P. ornatus population in the South-East Asian
 archipelago has important implications for the sustainable management of
 this lobster in that the species within the region needs to be managed as
 one genetic stock.
description: THE ORNATE SPINY LOBSTER
 P. ORNATUS IN THE SOUTH-EAST ASIAN ARCHIPELAGOOrnatus_Sampling_Figure_01_add_ScaleBar_addSeas copy.tifTable S1Spatial distribution of control region haplotypes among Panulirus ornatus from six localities in the South-East Asian archipelago.Table S2Null allele frequencies in original dataset and after correction calculated by using FreeNA 3.0. Values above 10% are in bold.Table S3Summary table of analysis of molecular variance (AMOVA) describing the partitioning of genetic variation for six Panulirus ornatus populations in original dataset and after correction based on 10 microsatellite loci.Table S4Genetic differentiation between Panulirus ornatus from collection locations using pairwise FST for microsatellite loci in original dataset (lower value) and after correction (upper value). No significant value was found after correction using FDR.Table S5Migration rate (and standard errors) in each population that are migrants derived from other populations per generation.GenotypingMicrosatelliteGenotyping Data and Diversity Indices of ten microsatellitesGenotypingMicrosatellite_OR_CorrectedNullAlleleGenotyping Data and Diversity Indices of ten microsatellites after correcting null allelesStructureResults_OR_10000-100000Results from Structure Harvester and Barplot of P. ornatus populations with the burnin as 10% of run length (10,000 - 100,000)BA3output_OrnatusOutput from BayesAss for P. ornatus populationsOrnatus_189ind_HocTanDaoGenotyping_mtDNAcontrolregion_189individualBestModel_G1.26_Ornatus_189ind_213poSite_2014jun03Results from BestModel using MEGA6migrate_outfileInitial output from Migrate ver 3.6.4Arlequin_189ind_6pop_213site_2014sep10_mainResults using Arlequin based on 12,500 and 16,000 permutationsAlequin_12500&16000_permutations.zip