cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.648122
title: CIL:41361

description: The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows an overview of cytoplasm at left, with mitochondria, vesicles, and endoplasmic reticulum. At right is a portion of the nucleus. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648123
title: CIL:41365

description: The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 15.6 nm thick slice of the computed tomogram in the x-y plane, and shows a nucleolus containing granular components of various size and density. The images have a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648124.v1
title: CIL:41367

description: The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows the plasma membrane with cytoplasm above and medium below. Numerous vesicles are present in the cytoplasm. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648125.v1
title: CIL:39751

description: Astrocyte cell from live slices taken from the cortex layer 2/3 of an 8 month old GFAP mouse. In this mouse the enhanced green fluorescent protein (EGFP) under the control of the human glial fibrillary acidic protein (GFAP) promoter (line TgN(GFAP-EGFP)GFEC-FKi). In the image, you can clearly see cell the body, astrocyte cloud and astrocyte projection on vascular tube crossing astrocyte cloud (left and up from cell body). This image is the projection of 30 individual z slices. Single z slices from the stack are available as CIL 39752 and 39753.
DOI: 10.6084/m9.figshare.648120
title: CIL:41357

description: The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a region of cytoplasm containing 2 lysosomes. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation),the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648121
title: CIL:41358

description: The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows the details of a mitochondrion. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648122.v1
title: CIL:41361

description: The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows an overview of cytoplasm at left, with mitochondria, vesicles, and endoplasmic reticulum. At right is a portion of the nucleus. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648123.v1
title: CIL:41365

description: The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 15.6 nm thick slice of the computed tomogram in the x-y plane, and shows a nucleolus containing granular components of various size and density. The images have a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648119
title: CIL:41356

description: The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a region of cytoplasm containing a Golgi apparatus. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648120.v1
title: CIL:41357

description: The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a region of cytoplasm containing 2 lysosomes. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation),the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648121.v1
title: CIL:41358

description: The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows the details of a mitochondrion. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648116
title: CIL:41043

description: Transmission electron micrograph of a negatively stained preparation of ribosomes isolated from a 3-day old chick embryo that was maintained in a cold room for 24 hrs. The ribosome crystals seen in thin sections of these cells (see CIL:41041)are seen to be primarily tetramers.
DOI: 10.6084/m9.figshare.648117
title: CIL:41116

description: A transmission electron micrograph of a Leydig cell isolated from guinea pig testes. This is a higher magnification image of CIL: 41111 showing nuclear peripheral hererochromatin, highly organized smooth endoplasmic reticulum, and prominent mitochondria.
DOI: 10.6084/m9.figshare.648118
title: CIL:41355

description: A transmission electron micrograph of a Leydig cell isolated from guinea pig testes. This is a higher magnification image of CIL: 41116 highlighting the distinct nucleolus and a membrane-bound, debris-laden lipid vacuole.
DOI: 10.6084/m9.figshare.648118.v1
title: CIL:41355

description: A transmission electron micrograph of a Leydig cell isolated from guinea pig testes. This is a higher magnification image of CIL: 41116 highlighting the distinct nucleolus and a membrane-bound, debris-laden lipid vacuole.
DOI: 10.6084/m9.figshare.648119.v1
title: CIL:41356

description: The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a region of cytoplasm containing a Golgi apparatus. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
DOI: 10.6084/m9.figshare.648115
title: CIL:40564

description: Immunogold label specific for L1 antigen on DV-II. Monoclonal antibody to antigen L1 specifically labels DV-II and acidosomes but not lysosomes. Lysosomes are osmotically active so they tend to shrink when the cell is exposed to hyperosmotic fixative as occurred here. This vacuole was 5-8 min. old and contained 0.8µm latex beads. TEM taken on 3/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X.
DOI: 10.6084/m9.figshare.648117.v1
title: CIL:41116

description: A transmission electron micrograph of a Leydig cell isolated from guinea pig testes. This is a higher magnification image of CIL: 41111 showing nuclear peripheral hererochromatin, highly organized smooth endoplasmic reticulum, and prominent mitochondria.
DOI: 10.6084/m9.figshare.648114
title: CIL:40450

description: Transmission electron micrograph of a representative untreated vma9 mutant cell. VMA9 encodes a subunit of the vacuolar-type H+-ATPase (V-ATPase). vma9 mutant cells contain non-spherical vacuoles at a higher rate than wildtype cells. (8,000X). A sertraline-treated image of the same mutant is available as CIL: 40449.
DOI: 10.6084/m9.figshare.648115.v1
title: CIL:40564

description: Immunogold label specific for L1 antigen on DV-II. Monoclonal antibody to antigen L1 specifically labels DV-II and acidosomes but not lysosomes. Lysosomes are osmotically active so they tend to shrink when the cell is exposed to hyperosmotic fixative as occurred here. This vacuole was 5-8 min. old and contained 0.8µm latex beads. TEM taken on 3/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X.
DOI: 10.6084/m9.figshare.648111
title: CIL:40447

description: Transmission electron micrograph of a vacuole from a sertraline-treated vma9 mutant cell. The vacuole contains a concentric multilamellar body. (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648112
title: CIL:40448

description: Transmission electron micrograph of a polygonal vacuole from an untreated vma9 mutant cell. The vacuole contains a concentric multilamellar body. (40,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648113
title: CIL:40449

description: Transmission electron micrograph of a representative sertraline-treated vma9 mutant cell. VMA9 encodes a subunit of the vacuolar-type H+-ATPase (V-ATPase). Sertraline treatment is associated with an increase in electron lucency in vacuolar lumens. (8,000X). An untreated control image of the same mutant is available as CIL: 40450.
DOI: 10.6084/m9.figshare.648114.v1
title: CIL:40450

description: Transmission electron micrograph of a representative untreated vma9 mutant cell. VMA9 encodes a subunit of the vacuolar-type H+-ATPase (V-ATPase). vma9 mutant cells contain non-spherical vacuoles at a higher rate than wildtype cells. (8,000X). A sertraline-treated image of the same mutant is available as CIL: 40449.
DOI: 10.6084/m9.figshare.648110
title: CIL:40446

description: Transmission electron micrograph of a large vacuole from a sertraline-treated swa2 mutant cell. (12,500X). This image is part of a group of yeast vacuole images (CIL:40437-40448). The image contributor encourages Library users to post comments regarding the identity of the organelle in the lower right of the image.
DOI: 10.6084/m9.figshare.648111.v1
title: CIL:40447

description: Transmission electron micrograph of a vacuole from a sertraline-treated vma9 mutant cell. The vacuole contains a concentric multilamellar body. (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648112.v1
title: CIL:40448

description: Transmission electron micrograph of a polygonal vacuole from an untreated vma9 mutant cell. The vacuole contains a concentric multilamellar body. (40,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648113.v1
title: CIL:40449

description: Transmission electron micrograph of a representative sertraline-treated vma9 mutant cell. VMA9 encodes a subunit of the vacuolar-type H+-ATPase (V-ATPase). Sertraline treatment is associated with an increase in electron lucency in vacuolar lumens. (8,000X). An untreated control image of the same mutant is available as CIL: 40450.
DOI: 10.6084/m9.figshare.648107
title: CIL:40442

description: Transmission electron micrograph of a vacuole from a sertraline-treated drs2∆ mutant cell. This vacuole contains an undigested autophagosomes. This image is a high-magnification zoom of the cell in CIL40424 (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648109
title: CIL:40445

description: Transmission electron micrograph of a vacuole from a sertraline-treated sac1∆ mutant cell. The vacuolar membrane is moderately crenated. (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648110.v1
title: CIL:40446

description: Transmission electron micrograph of a large vacuole from a sertraline-treated swa2 mutant cell. (12,500X). This image is part of a group of yeast vacuole images (CIL:40437-40448). The image contributor encourages Library users to post comments regarding the identity of the organelle in the lower right of the image.
DOI: 10.6084/m9.figshare.648106
title: CIL:40441

description: Transmission electron micrograph of a vacuole from a sertraline-treated chc1 mutant cell. This vacuole shows evidence of active autophagy. (16,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448). The image contributor encourages Library users to post comments that address what causes the separation between the homogenous electron-opaque contents and the mixed opacity contents.
DOI: 10.6084/m9.figshare.648108
title: CIL:40443

description: Transmission electron micrograph of an oblong vacuole from an untreated drs2∆ mutant cell. (50,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648109.v1
title: CIL:40445

description: Transmission electron micrograph of a vacuole from a sertraline-treated sac1∆ mutant cell. The vacuolar membrane is moderately crenated. (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648106.v1
title: CIL:40441

description: Transmission electron micrograph of a vacuole from a sertraline-treated chc1 mutant cell. This vacuole shows evidence of active autophagy. (16,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448). The image contributor encourages Library users to post comments that address what causes the separation between the homogenous electron-opaque contents and the mixed opacity contents.
DOI: 10.6084/m9.figshare.648107.v1
title: CIL:40442

description: Transmission electron micrograph of a vacuole from a sertraline-treated drs2∆ mutant cell. This vacuole contains an undigested autophagosomes. This image is a high-magnification zoom of the cell in CIL40424 (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648108.v1
title: CIL:40443

description: Transmission electron micrograph of an oblong vacuole from an untreated drs2∆ mutant cell. (50,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648103
title: CIL:40436

description: Transmission electron micrograph of a representative untreated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. swa2 mutant cells exhibit a vacuolar fragmentation phenotype. (6,300X). An image of the same mutant following sertraline treatment is available as CIL 40435.
DOI: 10.6084/m9.figshare.648104
title: CIL:40437

description: Transmission electron micrograph of a vacuole from a sertraline-treated BY4716 wildtype cell. (16,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648105
title: CIL:40438

description: Transmission electron micrograph of an electron-lucent vacuole from a sertraline-treated BY4716 wildtype cell. (31,500X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648102
title: CIL:40435

description: Transmission electron micrograph of a representative sertraline-treated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. The vacuolar fragmentation phenotype is reversed by sertraline treatment. (6,300X). An image of the untreated mutant is available as CIL 40436.
DOI: 10.6084/m9.figshare.648103.v1
title: CIL:40436

description: Transmission electron micrograph of a representative untreated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. swa2 mutant cells exhibit a vacuolar fragmentation phenotype. (6,300X). An image of the same mutant following sertraline treatment is available as CIL 40435.
DOI: 10.6084/m9.figshare.648104.v1
title: CIL:40437

description: Transmission electron micrograph of a vacuole from a sertraline-treated BY4716 wildtype cell. (16,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648105.v1
title: CIL:40438

description: Transmission electron micrograph of an electron-lucent vacuole from a sertraline-treated BY4716 wildtype cell. (31,500X). This image is part of a group of yeast vacuole images (CIL:40437-40448).
DOI: 10.6084/m9.figshare.648099
title: CIL:40432

description: Transmission electron micrograph of a representative untreated wildtype prototrophic cell (derived from S288c). A large vacuole containing multiple electron-lucent inclusions is present. (8,000X). A similar cell treated with sertraline is available as CIL 40431.
DOI: 10.6084/m9.figshare.648100
title: CIL:40433

description: Transmission electron micrograph of a representative sertraline-treated sac1∆ mutant cell. SAC1 encodes a phosphatase that converts phosphatidylinositol 4-phosphate (PIP) to phosphatidylinositol (PI). (8,000X). An untreated image of the same mutant is available as CIL 40434.
DOI: 10.6084/m9.figshare.648101
title: CIL:40434

description: Tranmission electron micrograph of a representative untreated sac1∆ mutant cell. SAC1 encodes a phosphatase that converts phosphatidylinositol 4-phosphate (PIP) to phosphatidylinositol (PI). sac1∆ mutant cells have fewer normal vacuoles than wildtype. (10,000X). An image of the same mutant treated with sertraline is available as CIL 40433.
DOI: 10.6084/m9.figshare.648102.v1
title: CIL:40435

description: Transmission electron micrograph of a representative sertraline-treated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. The vacuolar fragmentation phenotype is reversed by sertraline treatment. (6,300X). An image of the untreated mutant is available as CIL 40436.