DOI: 10.5061/dryad.cvdncjt9v
title: Phenotypic data for: Does size-selective harvesting erode adaptive potential to thermal stress?

description: Overharvesting is a serious threat to many fish populations. High
 mortality and directional selection on body size can cause evolutionary
 change in exploited populations, via selection for a specific phenotype
 and a potential reduction in phenotypic diversity. Whether the loss of
 phenotypic diversity that accompanies directional selection impairs
 response to environmental stress is not known. To address this question,
 we exposed three zebrafish selection lines to thermal stress. Two lines
 had experienced directional selection for (1) large and (2) small body
 size, and one was (3) subject to random removal of individuals with
 respect to body size (i.e. line with no directional selection). Selection
 lines were exposed to three temperatures (elevated, 34°C; ambient, 28°C;
 low, 22°C) to determine the response to an environmental stressor (thermal
 stress). We assessed differences among selection lines in their life
 history (growth and reproduction), physiological traits (metabolic rate
 and critical thermal max), and behaviour (activity and feeding behaviour)
 when reared at different temperatures. Lines experiencing directional
 selection (i.e., size selected) showed reduced growth rate and a shift in
 average phenotype in response to lower or elevated thermal stress compared
 with fish from the random-selected line. Our data indicate that
 populations exposed to directional selection can have a more limited
 capacity to respond to thermal stress compared with fish that experience a
 comparable reduction in population size (but without directional
 selection). Future studies should aim to understand the impacts of
 environmental stressors on natural fish stocks. 
description: # Phenotypic data for paper entitled: Does size-selective harvesting erode
 adaptive potential to thermal stress?
 Data for phenotypic data
 from thermal experiment, includes life history, physiological and
 behavioural data ## Description of the data and file structure Excel files
 of data used in experiment: NA= no data CTmax = Thermal maximum experiment
 -Run = run number of experiment, Box= acclimation box, Rearing_Temperature
 = experimental rearing temperature (°C), Temperature = CTmax, Observer =
 who observed experiment, Date = date of experiment, Selection line = line
 replicate, Treat = pooled line replicate, Scope = Aerobic scope (°C), Cage
 = cage replicate, Col = colour of tag LengthWeight = Growth rate data -pic
 = picture ID, ID = replicate number, Tank = tank replicate, Line = line
 replicate, Selection = pooled line replicate, Rep = inividual replicate,
 colour = colour of tag, T = temperature (°C), Week = week, weight = weight
 (g), Temperature = temperature (°C), Length = length (mm), Cage = cage
 replicate, rep_ID = replicate Behaviour = Activity, boldness and
 exploration -Tank = tank replicate, Line = pooled line replicate, Cage =
 cage replicate, Temp = temperature (°C), Rep1 = replicate, image_top =
 image name of top camera, image_side = image number side camera, date =
 date of experiment, exp_tank, tank replicate of experiment, colour =
 colour of tag, number of time emerged = number of time emerged, Time
 emerged =first time emergence (s) , Total time emerged = total time spent
 emerged (s), Time per emerge = average time spent emerged (s), Selection =
 pooled line replicate Feeding behaviour- feeding behaviour experiment
 -Line=pooled line replicate, rep =replicate, selection = line replicate,
 Temp = temperature (°C), Num_feed = number of feeds, Time_to_feed = time
 taken till first feed (s), Feed_Cat= time categroy (s), Fed =whether fish
 eats or doesnt, colour=colour of tag, Tank= tank replicate, cage= cage
 replicate, weight = weight (g) Metabolic = SMR, MMR, AAS -Photo = photo
 number, Length = length of fish (mm), Date = date of experiment, Time =
 time period of experiment, Temp = temperature (°C), Tank = tank replicate,
 Cage = cage replicate, Treat = pooled line replicate, Line = line
 replicate, Chamber.Respirometer = chamber replicate number, Weight =
 weight of fish (g), SMR = standard metabolic rate (mgO2 g−1 h−1), MMR =
 maximum metabolic rate (mgO2 g−1 h−1), r2 = r2 value, color = colour of
 tag Mortality = Deaths throughout experiment -Tank = tank replicate, Line
 = line replicate, Selection = pooled line replicate, Rep = replicate,
 Colour = tag colour, T = temperature (°C), Mortality = death or no death,
 Cage = cage replicate Fecundity = egg number -Line = line replicate,
 Selection = pooled line replicate, Rep_line = replicate, Box = breeding
 box, Fertilised = number of fertilised eggs, Unfertilised = number of
 unfertilised eggs, Total viable = fertlised + unfertilised eggs, Dead =
 dead eggs, Temp = temperature (°C), Date = date of experiment Egg data =
 Metrics used for eggs/larvae -Treat = pooled line replicate, Line = line
 replicate, Rep = replicate of spawning tank, Date = date of experiment,
 Well = well number on breeding plate, Egg(L) = egg diameter (um), Yolk(L)
 = yolk diameter (um), Larvae(L)= larvae length (um), LarvaeYolk(L) =
 larvae yolk diameter(um), Hatchdate= date of hatching, Pic = picture
 number, Temperature = temperature (°C), Hatchday = days until hatch,
 Hatched = hatched or not hatched, Mothers = mother ID. ##