DOI: 10.5061/dryad.rbnzs7hfg
title: Phytoplankton optical fingerprint libraries for development of phytoplankton ocean color satellite products

description: Quantifying changes in phytoplankton communities using ocean color is
 essential for predicting ocean food resources, occurrences of harmful
 algal blooms, and carbon and other elemental cycles, among other
 predictions.  Here we present a dataset of greater than fifty
 strains of phytoplankton, from a range of taxonomic lineages, geographic
 locations, and time in culture, alone and in mixtures, grown to
 exponential and/or stationary phase for determination of hyperspectral
 UV-VIS absorption coefficients, multi-angle and multi-spectral backscatter
 coefficients, volume scattering functions, particle size distributions,
 fluorescence, and hyperspectral remote sensing reflectance.  The
 measurements obtained from these experiments are valuable to facilitate
 development of new global and/or regional ocean color models by the
 broader scientific community. 
description: A range of experimental conditions were used
 to assess changes in cellular carbon, pigments, ratios and optical
 properties.  For most of the strains, measurements were made at two growth
 stages: mid-exponential (Exp) and stationary (Stat) growth phase, but for
 some strains, measurements were made during only one growth phase. A range
 of mixed cultures were made to assess the ability of algorithms derived
 from individual cultures to resolve the known algal components in the
 prepared mixtures. The potential for changes in pigment composition in
 response to <em>in vitro</em> culture adaption over time was
 assessed by studying multiple strains of the same species
 (<em>Amphidinium carterae</em>, <em>Ditylum
 brightwellii</em>, and <em>Heterosigma akashiwo</em>)
 isolated on different historical dates, but from similar geographic
 areas.  <em>Synechococcus </em>sp., <em>Thalassiosira
 oceanica</em>, and <em>Ostreococcus lucimarinus
 </em>were examined for their response in pigment composition,
 cellular carbon, and optical properties to ‘climate change scenarios’ of
 elevated temperature, irradiance and decreased pH.  More detail on these
 manipulations can be found in (Neeley et al. 2022).
 Methods are summarized here with more detail found in
 Neeley <em>et al.</em> (2022) and Lomas <em>et
 al.</em> (2023).  Samples for pigment concentration were analyzed by
 HPLC (Agilent RR1200 HPLC) and calculated from
 pigment response factors, extraction volumes determined from a Vitamin E
 acetate standard and filtration volumes.  Measurements of filter pad particle optical density
 (OD<sub>fp</sub>) were performed using a Cary
 4000 UV-Visible scanning spectrophotometer equipped with a 15 cm
 integrating sphere (Labsphere DRA-CA-900), betwee 290-850 nm with a 2nm
 Slit Band Width (SBW), 0.2 nm data interval, reduced slit height and 120
 nm per minute scan speed.
 Dissolved Organic Carbon measurements were made on Shimadzu TOC-L or TOC-
 V unit equipped with a total
 nitrogen analyzer using the high temperature combustion catalytic
 oxidation method. CDOM absorbance spectra are measured on a
 double-beam Cary 100 Ultraviolet-Visible scanning spectrophotometer using
 Suprasil®
 quartz cylindrical cells of 100 mm pathlength with ultraviolet oxidized
 Milli-Q water as the blank and reference.  Samples for particulate organic
 nitrogen and carbon concentrations were filtered onto 25 mm precombusted
 Ahlstrom glass fiber filters (nominal pore size 0.7 µm) under low pressure
 (~125 mm Hg), and rinsed with 0.2 µm-filtered dilution seawater.  Blanks
 were created by rinsing a pre-combusted filter with filtered dilution
 seawater to account for any DOC adsorption. A
 benchtop flow-through apparatus was constructed to measure the inherent
 optical properties (IOPs) of the diluted phytoplankton cultures. All
 optical data were processed as described in detail in Lomas et al.
 202x.  <strong>Usage Notes</strong> This
 dataset represents a taxonomically and geographically broad dataset of
 multiple phytoplankton optical parameters.  The dataset can be used to
 validate regional to global scale phytoplankton community composition
 models, as well as develop new models focused on resolving different
 phytoplankton within the same taxonomic lineage.
description: # README\_optics.txt --- Phytoplankton Optical Fingerprint Libraries for
 Development of Phytoplankton Ocean Color Satellite Products Michael W.
 Lomas1, Aimee R. Neeley2, Ryan Vandermeulen2,*, Antonio Mannino3, Crystal
 Thomas2, Michael G. Novak2, Scott A. Freeman2 1 Bigelow Institute of Ocean
 Sciences, Bigelow Laboratory for Ocean 6Sciences, East Boothbay, ME 04544,
 USA 2 NASA Goddard Space Flight Center/Science Systems and Applications
 Inc., Greenbelt, Maryland 20771, USA 3 NASA Goddard Space Flight Center,
 Greenbelt, Maryland 20771, USA * Current Address: NOAA National Marine
 Fisheries Service, Silver Spring, Maryland 20910, USA. Contact Michael W.
 Lomas ()for any questions about the data. \----
 Description ---- The dataset comprises bio-optical data collected from 50+
 pure phytoplankton cultures that represent broad taxonomic diversity from
 a broad geographic range of original isolation. Data were collected from
 phytoplankton cultures in exponential and stationary growth phase, from
 different known mixtures of cultures, from cultures with different
 durations in culture, and under climate change growth conditions of
 elevated temperature, irradiance and CO2. The dataset includes the
 following fields: I. ap_ACS worksheet: particle absorption coefficients
 from ac-s instrument 1. EXP: Experiment ID 2. CCMP: Strain #### designated
 by CCMP 3. Strain name(s): 4. Oceanic/Coastal: 5. Class: Phytoplankton
 strain taxonomic class 6. Phase: Growth phase of culture when sampled 7.
 Dilution: Designation of dilution 8. Date (MM/DD/YYYY): Date of experiment
 9. Date (YYYYMMDD): Date of experiment 10-142. ap-ACS ### (m-1): ac-s
 particle absorption coefficient every 2.5nm between wavelengths of
 400-730nm inclusive in units of m-1. II. cp_ACS worksheet: particle beam
 attenuation coefficients from ac-s 1. EXP: Experiment ID 2. CCMP: Strain
 #### designated by CCMP 3. Strain name(s): 4. Oceanic/Coastal: 5. Class:
 Phytoplankton strain taxonomic class 6. Phase: Growth phase of culture
 when sampled 7. Dilution: Designation of dilution 8. Date (MM/DD/YYYY):
 Date of experiment 9. Date (YYYYMMDD): Date of experiment 10-142. cp ###
 (m-1): ac-s beam attenuation coefficient every 2.5nm between wavelengths
 of 400-730nm inclusive in units of m-1. III. Backscatter worksheet:
 particulate scattering coefficients 1. EXP: Experiment ID 2. CCMP: Strain
 #### designated by CCMP 3. Strain name(s): 4. Oceanic/Coastal: 5. Class:
 Phytoplankton strain taxonomic class 6. Phase: Growth phase of culture
 when sampled 7. Dilution: Designation of dilution 8. Date (MM/DD/YYYY):
 Date of experiment 9. Date (YYYYMMDD): Date of experiment 10. bb/b: ratio
 of backscattering coefficient to scattering coefficient 11. bb/b - stdev:
 standard deviation of ratio of backscattering coefficient to scattering
 coefficient 12. HS6-bb 375 (m-1): particulate backscatter coefficient at
 wavelength ### from Hydroscat-6 13. HS6-bb 440 (m-1): particulate
 backscatter coefficient at wavelength ### from Hydroscat-6 14. HS6-bb 488
 (m-1): particulate backscatter coefficient at wavelength ### from
 Hydroscat-6 15. HS6-bb 550 (m-1): particulate backscatter coefficient at
 wavelength ### from Hydroscat-6 16. HS6-bb 620 (m-1): particulate
 backscatter coefficient at wavelength ### from Hydroscat-6 17. HS6-bb 700
 (m-1): particulate backscatter coefficient at wavelength ### from
 Hydroscat-6 18. VSF3-bb 440 (m-1): particulate backscatter coefficient at
 wavelength ### from VSF-3 19. VSF3-bb 532 (m-1): particulate backscatter
 coefficient at wavelength ### from VSF-3 20. VSF3-bb 660 (m-1):
 particulate backscatter coefficient at wavelength ### from VSF-3 21.
 VSFR-bb 650 (m-1): particulate backscatter coefficient at wavelength ###
 from VSF-red 22. BB3-bb 470 (m-1): particulate backscatter coefficient at
 wavelength ### from BB3 23. BB3-bb 532 (m-1): particulate backscatter
 coefficient at wavelength ### from BB3 24. BB3-bb 660 (m-1): particulate
 backscatter coefficient at wavelength ### from BB9 25. BB9-bb 409 (m-1):
 particulate backscatter coefficient at wavelength ### from BB9 26. BB9-bb
 441 (m-1): particulate backscatter coefficient at wavelength ### from BB9
 27. BB9-bb 488 (m-1): particulate backscatter coefficient at wavelength
 ### from BB9 28. BB9-bb 508 (m-1): particulate backscatter coefficient at
 wavelength ### from BB9 29. BB9-bb 526 (m-1): particulate backscatter
 coefficient at wavelength ### from BB9 30. BB9-bb 594 (m-1): particulate
 backscatter coefficient at wavelength ### from BB9 31. BB9-bb 652 (m-1):
 particulate backscatter coefficient at wavelength ### from BB9 32. BB9-bb
 679 (m-1): particulate backscatter coefficient at wavelength ### from BB9
 33. BB9-bb 717 (m-1): particulate backscatter coefficient at wavelength
 ### from BB9 IV. LISST worksheet: phytoplankton size distribution and
 bio-volume (LISST) 1. EXP: Experiment ID 2. CCMP: Strain #### designated
 by CCMP 3. Strain name(s): 4. Oceanic/Coastal: 5. Class: Phytoplankton
 strain taxonomic class 6. Phase: Growth phase of culture when sampled 7.
 Dilution: Designation of dilution 8. Date (MM/DD/YYYY): Date of experiment
 9. Date (YYYYMMDD): Date of experiment 10. Volume (mL L-1) 1.36: Particle
 volume in specified size bin ### um 11. Volume (mL L-1) 1.6: Particle
 volume in specified size bin ### um 12. Volume (mL L-1) 1.89: Particle
 volume in specified size bin ### um 13. Volume (mL L-1) 2.23: Particle
 volume in specified size bin ### um 14. Volume (mL L-1) 2.63: Particle
 volume in specified size bin ### um 15. Volume (mL L-1) 3.11: Particle
 volume in specified size bin ### um 16. Volume (mL L-1) 3.67: Particle
 volume in specified size bin ### um 17. Volume (mL L-1) 4.33: Particle
 volume in specified size bin ### um 18. Volume (mL L-1) 5.11: Particle
 volume in specified size bin ### um 19. Volume (mL L-1) 6.03: Particle
 volume in specified size bin ### um 20. Volume (mL L-1) 7.11: Particle
 volume in specified size bin ### um 21. Volume (mL L-1) 8.39: Particle
 volume in specified size bin ### um 22. Volume (mL L-1) 9.9: Particle
 volume in specified size bin ### um 23. Volume (mL L-1) 11.7: Particle
 volume in specified size bin ### um 24. Volume (mL L-1) 13.8: Particle
 volume in specified size bin ### um 25. Volume (mL L-1) 16.3: Particle
 volume in specified size bin ### um 26. Volume (mL L-1) 19.2: Particle
 volume in specified size bin ### um 27. Volume (mL L-1) 22.7: Particle
 volume in specified size bin ### um 28. Volume (mL L-1) 26.7: Particle
 volume in specified size bin ### um 29. Volume (mL L-1) 31.6: Particle
 volume in specified size bin ### um 30. Volume (mL L-1) 37.2: Particle
 volume in specified size bin ### um 31. Volume (mL L-1) 43.9: Particle
 volume in specified size bin ### um 32. Volume (mL L-1) 51.9: Particle
 volume in specified size bin ### um 33. Volume (mL L-1) 61.2: Particle
 volume in specified size bin ### um 34. Volume (mL L-1) 72.2: Particle
 volume in specified size bin ### um 35. Volume (mL L-1) 85.2: Particle
 volume in specified size bin ### um 36. Volume (mL L-1) 101: Particle
 volume in specified size bin ### um 37. Volume (mL L-1) 119: Particle
 volume in specified size bin ### um 38. Volume (mL L-1) 140: Particle
 volume in specified size bin ### um 39. Volume (mL L-1) 165: Particle
 volume in specified size bin ### um 40. Volume (mL L-1) 195: Particle
 volume in specified size bin ### um 41. Volume (mL L-1) 230: Particle
 volume in specified size bin ### um 42. N(D) (particles m-3) 1.36: Number
 of particles per m3 in specified size bin ### um 43. N(D) (particles m-3)
 1.6: Number of particles per m3 in specified size bin ### um 44. N(D)
 (particles m-3) 1.89: Number of particles per m3 in specified size bin ###
 um 45. N(D) (particles m-3) 2.23: Number of particles per m3 in specified
 size bin ### um 46. N(D) (particles m-3) 2.63: Number of particles per m3
 in specified size bin ### um 47. N(D) (particles m-3) 3.11: Number of
 particles per m3 in specified size bin ### um 48. N(D) (particles m-3)
 3.67: Number of particles per m3 in specified size bin ### um 49. N(D)
 (particles m-3) 4.33: Number of particles per m3 in specified size bin ###
 um 50. N(D) (particles m-3) 5.11: Number of particles per m3 in specified
 size bin ### um 51. N(D) (particles m-3) 6.03: Number of particles per m3
 in specified size bin ### um 52. N(D) (particles m-3) 7.11: Number of
 particles per m3 in specified size bin ### um 53. N(D) (particles m-3)
 8.39: Number of particles per m3 in specified size bin ### um 54. N(D)
 (particles m-3) 9.9: Number of particles per m3 in specified size bin ###
 um 55. N(D) (particles m-3) 11.7: Number of particles per m3 in specified
 size bin ### um 56. N(D) (particles m-3) 13.8: Number of particles per m3
 in specified size bin ### um 57. N(D) (particles m-3) 16.3: Number of
 particles per m3 in specified size bin ### um 58. N(D) (particles m-3)
 19.2: Number of particles per m3 in specified size bin ### um 59. N(D)
 (particles m-3) 22.7: Number of particles per m3 in specified size bin ###
 um 60. N(D) (particles m-3) 26.7: Number of particles per m3 in specified
 size bin ### um 61. N(D) (particles m-3) 31.6: Number of particles per m3
 in specified size bin ### um 62. N(D) (particles m-3) 37.2: Number of
 particles per m3 in specified size bin ### um 63. N(D) (particles m-3)
 43.9: Number of particles per m3 in specified size bin ### um 64. N(D)
 (particles m-3) 51.9: Number of particles per m3 in specified size bin ###
 um 65. N(D) (particles m-3) 61.2: Number of particles per m3 in specified
 size bin ### um 66. N(D) (particles m-3) 72.2: Number of particles per m3
 in specified size bin ### um 67. N(D) (particles m-3) 85.2: Number of
 particles per m3 in specified size bin ### um 68. N(D) (particles m-3)
 101: Number of particles per m3 in specified size bin ### um 69. N(D)
 (particles m-3) 119: Number of particles per m3 in specified size bin ###
 um 70. N(D) (particles m-3) 140: Number of particles per m3 in specified
 size bin ### um 71. N(D) (particles m-3) 165: Number of particles per m3
 in specified size bin ### um 72. N(D) (particles m-3) 195: Number of
 particles per m3 in specified size bin ### um 73. N(D) (particles m-3)
 230: Number of particles per m3 in specified size bin ### um 74. A(D) (m2
 m-3) 1.36: Surface area in m2 of particles per m3 in specified size bin
 ### um 75. A(D) (m2 m-3) 1.6: Surface area in m2 of particles per m3 in
 specified size bin ### um 76. A(D) (m2 m-3) 1.89: Surface area in m2 of
 particles per m3 in specified size bin ### um 77. A(D) (m2 m-3) 2.23:
 Surface area in m2 of particles per m3 in specified size bin ### um 78.
 A(D) (m2 m-3) 2.63: Surface area in m2 of particles per m3 in specified
 size bin ### um 79. A(D) (m2 m-3) 3.11: Surface area in m2 of particles
 per m3 in specified size bin ### um 80. A(D) (m2 m-3) 3.67: Surface area
 in m2 of particles per m3 in specified size bin ### um 81. A(D) (m2 m-3)
 4.33: Surface area in m2 of particles per m3 in specified size bin ### um
 82. A(D) (m2 m-3) 5.11: Surface area in m2 of particles per m3 in
 specified size bin ### um 83. A(D) (m2 m-3) 6.03: Surface area in m2 of
 particles per m3 in specified size bin ### um 84. A(D) (m2 m-3) 7.11:
 Surface area in m2 of particles per m3 in specified size bin ### um 85.
 A(D) (m2 m-3) 8.39: Surface area in m2 of particles per m3 in specified
 size bin ### um 86. A(D) (m2 m-3) 9.9: Surface area in m2 of particles per
 m3 in specified size bin ### um 87. A(D) (m2 m-3) 11.7: Surface area in m2
 of particles per m3 in specified size bin ### um 88. A(D) (m2 m-3) 13.8:
 Surface area in m2 of particles per m3 in specified size bin ### um 89.
 A(D) (m2 m-3) 16.3: Surface area in m2 of particles per m3 in specified
 size bin ### um 90. A(D) (m2 m-3) 19.2: Surface area in m2 of particles
 per m3 in specified size bin ### um 91. A(D) (m2 m-3) 22.7: Surface area
 in m2 of particles per m3 in specified size bin ### um 92. A(D) (m2 m-3)
 26.7: Surface area in m2 of particles per m3 in specified size bin ### um
 93. A(D) (m2 m-3) 31.6: Surface area in m2 of particles per m3 in
 specified size bin ### um 94. A(D) (m2 m-3) 37.2: Surface area in m2 of
 particles per m3 in specified size bin ### um 95. A(D) (m2 m-3) 43.9:
 Surface area in m2 of particles per m3 in specified size bin ### um 96.
 A(D) (m2 m-3) 51.9: Surface area in m2 of particles per m3 in specified
 size bin ### um 97. A(D) (m2 m-3) 61.2: Surface area in m2 of particles
 per m3 in specified size bin ### um 98. A(D) (m2 m-3) 72.2: Surface area
 in m2 of particles per m3 in specified size bin ### um 99. A(D) (m2 m-3)
 85.2: Surface area in m2 of particles per m3 in specified size bin ### um
 100. A(D) (m2 m-3) 101: Surface area in m2 of particles per m3 in
 specified size bin ### um 101. A(D) (m2 m-3) 119: Surface area in m2 of
 particles per m3 in specified size bin ### um 102. A(D) (m2 m-3) 140:
 Surface area in m2 of particles per m3 in specified size bin ### um 103.
 A(D) (m2 m-3) 165: Surface area in m2 of particles per m3 in specified
 size bin ### um 104. A(D) (m2 m-3) 195: Surface area in m2 of particles
 per m3 in specified size bin ### um 105. A(D) (m2 m-3) 230: Surface area
 in m2 of particles per m3 in specified size bin ### um 106. N(D)_slope:
 Gamma = slope of the particle size distribution unitless 107. Average
 Diameter um: LISST 100x particle mean diameter 108. Beam_C_LISST 665
 (m-1): beam attenuation at 665 nm from LISST-100X V. POC and Chlorophyll-a
 worksheet (POC_chla): 1. EXP: Experiment ID 2. CCMP: Strain ####
 designated by CCMP 3. Strain name(s): 4. Oceanic/Coastal: 5. Class:
 Phytoplankton strain taxonomic class 6. Phase: Growth phase of culture
 when sampled 7. Dilution: Designation of dilution 8. Date (MM/DD/YYYY):
 Date of experiment 9. Date (YYYYMMDD): Date of experiment 10. Cells/L:
 Number of phytoplankton cells per Liter 11. POC (ug/L): Particulate
 Organic Carbon in ug/L 12. POC_SD (ug/L): Standard deviation of POC 13.
 POC:Tchla (ug/ug): Ratio of POC to total chlorophyll-a 14. POC:Tchla_SD
 (ug/ug): Standard deviation of ratio 15. TChl a (mg/m^3): Pigment
 concentration in mg/m^3 16. Tchla_SD: Standard deviation of pigment
 concentration in mg/m^3 VI. HPLC Pigments and ratios worksheet (HPLC): 1.
 EXP: Experiment ID 2. CCMP: Strain #### designated by CCMP 3. Strain
 name(s): 4. Oceanic/Coastal: 5. Class: Phytoplankton strain taxonomic
 class 6. Phase: Growth phase of culture when sampled 7. Dilution:
 Designation of dilution 8. Date (MM/DD/YYYY): Date of experiment 9. Date
 (YYYYMMDD): Date of experiment 10. TChl a: Pigment concentration in mg/m^3
 11. Tchla_SD: Standard deviation of pigment concentration in mg/m^3 12.
 TChl b: Pigment concentration in mg/m^3 13. TChlb_SD: Standard deviation
 of pigment concentration in mg/m^3 14. TChl c: Pigment concentration in
 mg/m^3 15. TChlc_SD: Standard deviation of pigment concentration in mg/m^3
 16. Car: Pigment concentration in mg/m^3 17. Car_SD: Standard deviation of
 pigment concentration in mg/m^3 18. But fuco: Pigment concentration in
 mg/m^3 19. Butfuco_SD: Standard deviation of pigment concentration in
 mg/m^3 20. Hex fuco: Pigment concentration in mg/m^3 21. Hex fuco_SD:
 Standard deviation of pigment concentration in mg/m^3 22. Allo: Pigment
 concentration in mg/m^3 23. Allo_SD: Standard deviation of pigment
 concentration in mg/m^3 24. Diad: Pigment concentration in mg/m^3 25.
 Diad_SD: Standard deviation of pigment concentration in mg/m^3 26. Diato:
 Pigment concentration in mg/m^3 27. Diato_SD: Standard deviation of
 pigment concentration in mg/m^3 28. Fuco: Pigment concentration in mg/m^3
 29. Fuco_SD: Standard deviation of pigment concentration in mg/m^3 30.
 Perid: Pigment concentration in mg/m^3 31. Peri_SD: Standard deviation of
 pigment concentration in mg/m^3 32. Zea: Pigment concentration in mg/m^3
 33. Zea_SD: Standard deviation of pigment concentration in mg/m^3 34. Chl
 a: Pigment concentration in mg/m^3 35. Chl a_SD: Standard deviation of
 pigment concentration in mg/m^3 36. DVChl a: Pigment concentration in
 mg/m^3 37. DVChl a_SD: Standard deviation of pigment concentration in
 mg/m^3 38. Chl b: Pigment concentration in mg/m^3 39. Chl b_SD: Standard
 deviation of pigment concentration in mg/m^3 40. DVChl b: Pigment
 concentration in mg/m^3 41. DVChl b_SD: Standard deviation of pigment
 concentration in mg/m^3 42. Chl c1c2: Pigment concentration in mg/m^3 43.
 Chl c1c2_SD: Standard deviation of pigment concentration in mg/m^3 44. Chl
 c3: Pigment concentration in mg/m^3 45. Chl c3_SD: Standard deviation of
 pigment concentration in mg/m^3 46. Lut: Pigment concentration in mg/m^3
 47. Lut_SD: Standard deviation of pigment concentration in mg/m^3 48. Neo:
 Pigment concentration in mg/m^3 49. Neo_SD: Standard deviation of pigment
 concentration in mg/m^3 50. Viola: Pigment concentration in mg/m^3 51.
 Viola_SD: Standard deviation of pigment concentration in mg/m^3 52. Phytin
 a: Pigment concentration in mg/m^3 53. Phytin a_SD: Standard deviation of
 pigment concentration in mg/m^3 54. Phide a: Pigment concentration in
 mg/m^3 55. Phide a_SD: Standard deviation of pigment concentration in
 mg/m^3 56. Pras: Pigment concentration in mg/m^3 57. Pras_SD: Standard
 deviation of pigment concentration in mg/m^3 58. \[Gyr diester]: Pigment
 concentration in mg/m^3 59. Gyr diester_SD: Standard deviation of pigment
 concentration in mg/m^3 60. Loroxanthin: Pigment concentration in mg/m^3
 61. Loro_SD: Standard deviation of pigment concentration in mg/m^3 VII.
 Filter pad integrating sphere particle absorption worksheet: 1. EXP:
 Experiment ID 2. CCMP: Strain #### designated by CCMP 3. Strain name(s):
 4. Oceanic/Coastal: 5. Class: Phytoplankton strain taxonomic class 6.
 Phase: Growth phase of culture when sampled 7. Dilution: Designation of
 dilution 8. Date (MM/DD/YYYY): Date of experiment 9. Date (YYYYMMDD): Date
 of experiment 10-2510. ###.# (m-1): particle absorption coefficient at
 wavelength every 0.2nm between 300-800nm inclusive in units of m-1. VIII.
 Filter pad integrating sphere phytoplankton absorption worksheet
 (aph-spec): 1. EXP: Experiment ID 2. CCMP: Strain #### designated by CCMP
 3. Strain name(s): 4. Oceanic/Coastal: 5. Class: Phytoplankton strain
 taxonomic class 6. Phase: Growth phase of culture when sampled 7.
 Dilution: Designation of dilution 8. Date (MM/DD/YYYY): Date of experiment
 9. Date (YYYYMMDD): Date of experiment 10-2510. ###.# (m-1): standard
 deviation of particle absorption coefficient every 0.2 nm between
 wavelengths of 300.0-800.0 nm inclusive in units of m-1. IX. Filter pad
 integrating sphere de-pigmented particle absorption worksheet (ad-spec):
 1. EXP: Experiment ID 2. CCMP: Strain #### designated by CCMP 3. Strain
 name(s): 4. Oceanic/Coastal: 5. Class: Phytoplankton strain taxonomic
 class 6. Phase: Growth phase of culture when sampled 7. Dilution:
 Designation of dilution 8. Date (MM/DD/YYYY): Date of experiment 9. Date
 (YYYYMMDD): Date of experiment 10-2510. ###.# (m-1): de-pigmented particle
 absorption coefficient every 0.2 nm between wavelengths of 300.0-800.0 nm
 inclusive in units of m-1. X. Filter pad integrating sphere de-pigmented
 particle absorption uncertainty worksheet (ad_spec_unc): 1. EXP:
 Experiment ID 2. CCMP: Strain #### designated by CCMP 3. Strain name(s):
 4. Oceanic/Coastal: 5. Class: Phytoplankton strain taxonomic class 6.
 Phase: Growth phase of culture when sampled 7. Dilution: Designation of
 dilution 8. Date (MM/DD/YYYY): Date of experiment 9. Date (YYYYMMDD): Date
 of experiment 10-2510. ###.# (m-1): standard deviation of de-pigmented
 particle absorption coefficient every 0.2 nm between wavelengths of
 300.0-800.0 nm inclusive in units of m-1. XI. Filter pad integrating
 sphere phytoplankton absorption worksheet (aph-spec): 1. EXP: Experiment
 ID 2. CCMP: Strain #### designated by CCMP 3. Strain name(s): 4.
 Oceanic/Coastal: 5. Class: Phytoplankton strain taxonomic class 6. Phase:
 Growth phase of culture when sampled 7. Dilution: Designation of dilution
 8. Date (MM/DD/YYYY): Date of experiment 9. Date (YYYYMMDD): Date of
 experiment 10-2510. ###.# (m-1): Phytoplankton absorption coefficient
 every 0.2 nm between wavelengths of 300.0-800.0 nm inclusive in units of
 m-1. XII. Filter pad integrating sphere phytoplankton absorption
 uncertainty worksheet (aph_spec_unc): 1. EXP: Experiment ID 2. CCMP:
 Strain #### designated by CCMP 3. Strain name(s): 4. Oceanic/Coastal: 5.
 Class: Phytoplankton strain taxonomic class 6. Phase: Growth phase of
 culture when sampled 7. Dilution: Designation of dilution 8. Date
 (MM/DD/YYYY): Date of experiment 9. Date (YYYYMMDD): Date of experiment
 10-2510. ###.# (m-1): Standard deviation of Phytoplankton absorption
 coefficient every 0.2 nm between wavelengths of 300.0-800.0 nm inclusive
 in units of m-1. When possible, replicate analyses were conducted for each
 sample type. Here, in this paper, we report the mean values of the
 replicate samples. For all the sample data, "NA" indicate no
 data or not defined.