DOI: 10.5061/dryad.4m9j1
title: Data from: Population structure of Guyanagaster necrorhizus supports termite dispersal for this enigmatic fungus

description: Understanding dispersal mechanisms—the movement of propagules—can shed
 light on how organisms are adapted for their ecosystem. Guyanagaster
 necrorhizus is a sequestrate fungus, meaning its dispersal propagules, or
 spores, are entirely enclosed within a fruiting body, termed a sporocarp.
 This fungus is most closely related to Armillaria and its allies. While
 Armillaria species form mushrooms with forcibly discharged spores, G.
 necrorhizus spores have lost this ability, and by necessity, must be
 passively dispersed. However, G. necrorhizus does not possess
 characteristics of other sequestrate fungi with known dispersal
 mechanisms. Repeated observations of termites feeding on G. necrorhizus
 sporocarps, and spores adhering to their exoskeleton, led to the
 hypothesis that termites disperse G. necrorhizus spores. To test this
 hypothesis, we used microsatellite markers and implemented population
 genetics analyses to understand patterns of clonality and population
 structure of G. necrorhizus. While Armillaria individuals can spread
 vegetatively over large areas, high genotypic diversity in G. necrorhizus
 populations suggest spores are the primary mode of dispersal, consistent
 with termite dispersal. Spatial genetic structure analyses suggest that G.
 necrorhizus sporocarps within 238 m of each other are more closely related
 than would be expected by chance. Conservative estimates from population
 assignment tests suggest populations separated by two km no longer
 exchange genes. Patterns of spatial genetic structure and population
 structure are consistent with previous studies analyzing foraging
 distances of termites found associated with G. necrorhizus sporocarps.
 Termites have rarely been recorded to specifically target fungal
 sporocarps, making this a potentially novel fungal-insect interaction.
description: Alleles for each
 individual at each locusAlleles for each locus
 for each individual are presented in this file. These alleles were used as
 the input for the rest of the analyses.Alleles.docxSTRUCTURE input fileThis is the file used as input for the STRUCTURE analysis; the rest of the details for this analysis are presented in the methods section.Guyanagaster_STRUCTURE.txtTESS input fileThis was the input file used for the TESS analysis; all other parameters used in this study are included in the methods.TESS.txtGenepop input fileThis is the file used for the Genepop analysis. Extra zeros at the end of the individual were used to keep the columns straight. Results are presented in the manuscript.Genepop.txtSTRUCTURE_CLUMPP2These are the results from the STRUCTURE analysis after combining the runs using CLUMPP. The cluster membership percentage for each individual is presented.TESS_CLUMPP_8These are the results from the TESS analysis after combining the runs using CLUMPP. The cluster membership percentage for each individual is presented.Spagedi_SUSSEN_AIRSTRIPHere is the input for the Spagedi analysis for the Sussen and Airstrip populations. The coordinates in this file were used in the GenClone analysis.SUSSEN_AIRSTRIP_FULL.txtpotaro_basecamp_SpagediThis is the input file used in the Spagedi analysis for the Potaro and Basecamp populations. The coordinates in this analysis were also used in the GenClone analysis.