cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.10438253
title: Effects of Heat Stress on the Development of GABAergic Neurons in the HPG Axis of Wenchang Chicks

description: ABSTRACT This study conducted an in-depth investigation on the development of GABAergic neurons and their receptors in HPG axis-related target organs of Wenchang chicks under heat stress. One-day-old healthy Wenchang chicks were randomly divided into control (CK) and heat stress (HS) groups. Chicks in the HS group were placed in a 40±0.5°C climatic chamber for HS treatment from 13:00 to 15:00 daily. By immunohistochemistry and Western blotting, GABA and GABAA receptor (GABAAR) expression in the hypothalamus of the HS group was significantly higher (p&lt;0.05), but GABAB receptor (GABABR) expression was significantly lower than that of the CK group (p&lt;0.05). Expression of GABA and its two receptors in the pituitary tissues of the HS group was significantly lower than in the CK group (p&lt;0.05). Expression of GABA and GABABR in ovaries in the HS group was significantly higher, but expression of GABAAR in the testes of the HS group was lower than that of the CK group (p&lt;0.05). In the male chicks, expression of GABA and its two receptors in the hypothalamus, pituitary, and testicular tissues of the HS group was significantly higher than that of the CK group (p&lt;0.05). Western blotting showed that the GABAAR and GABABR expression of the HS group was significantly higher than that of the CK group at 3 and 5 weeks of age. Thus, HS caused GABAergic nervous system disorder in the HPG axis of Wenchang chicks and seriously hindered the normal development of GABAergic neurons in chicks, leading to the disorder of the expression of GABA and its receptors in tissues.
DOI: 10.6084/m9.figshare.10438262.v1
title: Production of Passion fruit in a semi-hydroponic system under protected cultivation

description: Abstract The objective of this research was to evaluate the production of passion fruit (Passiflora edulis Sims) cultivars in different nutrient solutions, using commercial Bioplant® substrate in slabs system in protected cultivation. A randomized complete block design in a factorial scheme was used with 2 passion fruit cultivars (Araguari and Yellow Master) x 4 nutrient solutions (solution 1, solution 2, solution 3 and solution 4), each plot consisting of 6 plants with 6 replications. Electrical conductivity evaluations in the slabs drained solution started at 15 days after seedling transplantation and the nutrient contents were quantified at the end of the research. In passion fruit, ether extract, crude fiber and crude protein were evaluated. Fruit number, pulp mass, peel thickness and gas exchange were evaluated. It was concluded that the use of nutrient solution 2 in cultivar Yellow Master, with 2.72 dS m-1 electrical conductivity and the use of nutrient solution 4 in cultivar Araguari, with 2.95 dS m-1 electrical conductivity, yielded the largest number of fruits, pulp mass, fruit mass and gas exchange, in slabs system in the protected cultivation of passion fruit.
DOI: 10.6084/m9.figshare.10438244
title: Fruit quality of pineapple ‘Vitória’ under macronutrients and boron deficiency

description: Abstract The objective of this study was to evaluate the influence of macronutrient and boron deficiencies on physico-chemical characteristics of pineapple fruits, ‘Vitoria’ cultivar. The experimental design was a randomized complete block with eight treatments (complete solution, -N, -P, -K, -Ca, -Mg, -S and -B) and six replicates. The experiment was conducted from October 2016 to March 2018, and the variables evaluated were: fresh fruit and crown mass, fruit diameter, fruit and crown length, juice volume and mass, titratable acidity (TA), soluble solids (SS), vitamin C, juice pH and SS/TA ratio. All treatments with nutrient deficiency reduced the fresh fruit mass. In treatments -N and -Ca there was a reduction of 41.9 and 35.3%, respectively, in the crown fresh mass. Fruits diameter under N, K and Mg deficiency was lower, fruit length, soluble solids and juice pH were lower in treatment with N deficiency. Isolated deficiencies of each of the macronutrients and B reduced the mass of the whole fruit and the crownless fruit. The nutrient that most limited the size and quality of ‘Vitoria’ pineapple fruit was nitrogen.
DOI: 10.6084/m9.figshare.10438247
title: THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS

description: ABSTRACT Objective: To analyze the effects of sericin treatment, associated or not with swimming with load exercise, on initial sciatic nerve repair after compression in Wistar rats. Methods: Forty animals were divided into five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the axonotmesis procedure, the sericin was applied to the injury-sericin and injury-sericin-swimming groups. The injury-swimming and injury-sericin-swimming groups performed the swimming with load exercise for five days, beginning on the third postoperative day (PO), and were evaluated for function, nociception and allodynia. Euthanasia was performed on the 8th PO day and fragments of the nerve were collected and prepared for quantitative and descriptive analysis in relation to the total amount of viable nerve fibers and non-viable nerve fibers, nerve fiber diameter, axon diameter and myelin sheath thickness. Results: There was no significant improvement in the sciatic functional index up to the eighth day. The Von Frey test of the surgical scar and plantar fascia indicated a reduction in pain and allodynia for the injury-swimming and injury-sericin-swimming groups. The morphological analysis presented similar characteristics in the injury-sericin, injury-swimming and injury-sericin-swimming groups, but there was a significant difference in the number of smaller non-viable nerve fibers in the injury-swimming and injury-sericin-swimming groups as compared to the others. Conclusions: Isolated sericin protein presented proinflammatory characteristics. There was improvement of allodynia and a decrease in the pain at the site of the surgical incision, possibly linked to an aquatic effect. There was no acceleration of nerve repair on the eighth day after the injury. Level of Evidence I; High quality randomized clinical trial with or without statistically significant difference, but with narrow confidence intervals.
DOI: 10.6084/m9.figshare.10438253.v1
title: Effects of Heat Stress on the Development of GABAergic Neurons in the HPG Axis of Wenchang Chicks

description: ABSTRACT This study conducted an in-depth investigation on the development of GABAergic neurons and their receptors in HPG axis-related target organs of Wenchang chicks under heat stress. One-day-old healthy Wenchang chicks were randomly divided into control (CK) and heat stress (HS) groups. Chicks in the HS group were placed in a 40±0.5°C climatic chamber for HS treatment from 13:00 to 15:00 daily. By immunohistochemistry and Western blotting, GABA and GABAA receptor (GABAAR) expression in the hypothalamus of the HS group was significantly higher (p&lt;0.05), but GABAB receptor (GABABR) expression was significantly lower than that of the CK group (p&lt;0.05). Expression of GABA and its two receptors in the pituitary tissues of the HS group was significantly lower than in the CK group (p&lt;0.05). Expression of GABA and GABABR in ovaries in the HS group was significantly higher, but expression of GABAAR in the testes of the HS group was lower than that of the CK group (p&lt;0.05). In the male chicks, expression of GABA and its two receptors in the hypothalamus, pituitary, and testicular tissues of the HS group was significantly higher than that of the CK group (p&lt;0.05). Western blotting showed that the GABAAR and GABABR expression of the HS group was significantly higher than that of the CK group at 3 and 5 weeks of age. Thus, HS caused GABAergic nervous system disorder in the HPG axis of Wenchang chicks and seriously hindered the normal development of GABAergic neurons in chicks, leading to the disorder of the expression of GABA and its receptors in tissues.
DOI: 10.6084/m9.figshare.10438259.v1
title: Outcomes of segmental femoral artery pseudoaneurysm in patients with Behçet’s disease: a single center’s experience

description: Abstract Background Behçet's disease (BD) is an autoimmune condition that involves multiple systems. The most common arterial manifestation in BD patients is pseudoaneurysm, which has higher frequency than aneurysm formation. Objectives To clarify the importance of profunda femoris artery in BD pseudoaneurysm, and present a new method for identifying healthy segments for anastomosis. Methods Fifteen patients presented at a vascular department with pseudoaneurysms of the common femoral (CFA) and superficial femoral artery (SFA), were diagnosed with BD and underwent surgical intervention at Kasr al Ainy hospitals over 2 years. Results All patients were male. The patients ranged in age from 30 to 40 years (mean, 33.8±2.6 years). Mean duration of the disease was 5±3.4 years. Eight (53.3%) CFA graft interpositions, six (40%) mid SFA graft interpositions, and one (6.66) distal SFA graft interposition were performed. Eight (53.3%) of these operations were performed using Dacron graft and sartorius flap, three (20%) were performed with a polytetrafluoroethylene graft, and four (26.66) with a great saphenous vein graft. Two cases (13.33%) were accidentally discovered while four cases (26.66%) had short distance claudication, four cases (26.66%) had pain at rest, and five cases (33.33%) presented with pulsatile swelling correlated with pseudoaneurysm size (p = 0.005). Patients were followed-up over 1 year for new aneurysms and recurrence. Conclusions Surgical repair with muscle flap coverage, with or without ligation of profunda femoris artery, does not affect prognosis. An alternative method for identifying healthy segments of femoral artery may be frozen section and examination of the artery to decrease the risk of recurrence at the site of anastomosis.
DOI: 10.6084/m9.figshare.10438241
title: A systematic scoping review of the genetic ancestry of the Brazilian population

description: Abstract The genetic background of the Brazilian population is mainly characterized by three parental populations: European, African, and Native American. The aim of this study was to overview the genetic ancestry estimates for different Brazilian geographic regions and analyze factors involved in these estimates. In this systematic scoping review were included 51 studies, comprehending 81 populations of 19 states from five regions of Brazil. To reduce the potential of bias from studies with different sampling methods, we calculated the mean genetic ancestry weighted by the number of individuals. The weighted mean proportions of European, African, and Native American ancestries were 68.1%, 19.6%, and 11.6%, respectively. At the regional level, the highest European contribution occurred in the South, while the highest African and Native American contributions occurred in the Northeastern and Northern regions, respectively. Among states in the Northeast region, Bahia and Ceará showed significant differences, suggesting distinct demographic histories. This review contributes for a broader understanding of the Brazilian ancestry and indicates that the ancestry estimates are influenced by the type of molecular marker and the sampling method.
DOI: 10.6084/m9.figshare.10438235
title: Productive Performance, Egg Characteristics and Hatching Traits of Three Chicken Genotypes under Free-Range, Semi-Intensive, and Intensive Housing Systems

description: ABSTRACT The present study aimed at evaluating the effect of housing system on the live performance, egg quality, and hatching traits of three dual-purpose chicken genotypes. In total, 180 birds, comprising 48 pullets and 12 cockerels from each of three genotypes, were evaluated during the production phase (27-46 weeks). For this, 144 pullets and 36 cockerels were randomly picked from 18 treatment block groups were shifted to breeding coops, allotting 4 pullets to one cockerel. A completely randomized complete block design (RCBD) was employed. Three genotypes, purebred Naked Neck (NN) and two crosses Rhode Island Red × Naked Neck (RIR × NN = RNN) and Black Australorp × Naked Neck (BAL × NN = BNN), were compared. Intensive system (p&lt;0.0001) and BNN hens (p&lt;0.0001) were heavier on week 26 and 46. Higher egg production (p&lt;0.0001) was obtained in the intensive system and in BNN hens (p&lt;0.0001). Hens maintained in the intensive systems produced heavier eggs and higher egg mass (p&lt;0.0001), and RNN and BNN hens laid heavier eggs (p&lt;0.0001) while higher egg mass (p&lt;0.0001) was found in BNN hens. Higher egg shape index (initial, p=0.0002), egg surface area (initial, p&lt;0.0001; final, p&lt;0.0001), egg volume (initial, p&lt;0.0001; final, p&lt;0.0001) and Haugh unit score (initial, p=0.0002; final, p=&lt;0.0001) were obtained in RNN and BNN hens. At the end of the experiment (46 weeks), higher yolk index (p=0.0004) was found in RNN and BNN eggs, and thicker eggshells (p&lt;0.0001) in RNN eggs. Higher egg hatchability was obtained in the free-range system (p&lt;0.0001) and in the RNN genotype (p&lt;0.0001). The highest fertility rates were detected in the free-range system (p&lt;0.0001), and in the RNN and BNN genotypes (p&lt;0.0001). The lowest infertile egg rates were observed in the free-range system (p&lt;0.0001) and in RNN and BNN genotypes (p&lt;0.0001). The lowest dead-in-shell rate was calculated for the free-range system (p=0.0456). In conclusion, free-range and semi-intensive system largely influence productive performance, egg quality and hatching traits. Regarding genotypes, RNN and BNN crossbred hens perform better than NN purebreds.
DOI: 10.6084/m9.figshare.10438238
title: Artificial Intelligence in Cardiology: Concepts, Tools and Challenges - “The Horse is the One Who Runs, You Must Be the Jockey”

description: Abstract The recent advances at hardware level and the increasing requirement of personalization of care associated with the urgent needs of value creation for the patients has helped Artificial Intelligence (AI) to promote a significant paradigm shift in the most diverse areas of medical knowledge, particularly in Cardiology, for its ability to support decision-making and improve diagnostic and prognostic performance. In this context, the present work does a non-systematic review of the main papers published on AI in Cardiology, focusing on its main applications, potential impacts and challenges.
DOI: 10.6084/m9.figshare.10438247.v1
title: THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS

description: ABSTRACT Objective: To analyze the effects of sericin treatment, associated or not with swimming with load exercise, on initial sciatic nerve repair after compression in Wistar rats. Methods: Forty animals were divided into five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the axonotmesis procedure, the sericin was applied to the injury-sericin and injury-sericin-swimming groups. The injury-swimming and injury-sericin-swimming groups performed the swimming with load exercise for five days, beginning on the third postoperative day (PO), and were evaluated for function, nociception and allodynia. Euthanasia was performed on the 8th PO day and fragments of the nerve were collected and prepared for quantitative and descriptive analysis in relation to the total amount of viable nerve fibers and non-viable nerve fibers, nerve fiber diameter, axon diameter and myelin sheath thickness. Results: There was no significant improvement in the sciatic functional index up to the eighth day. The Von Frey test of the surgical scar and plantar fascia indicated a reduction in pain and allodynia for the injury-swimming and injury-sericin-swimming groups. The morphological analysis presented similar characteristics in the injury-sericin, injury-swimming and injury-sericin-swimming groups, but there was a significant difference in the number of smaller non-viable nerve fibers in the injury-swimming and injury-sericin-swimming groups as compared to the others. Conclusions: Isolated sericin protein presented proinflammatory characteristics. There was improvement of allodynia and a decrease in the pain at the site of the surgical incision, possibly linked to an aquatic effect. There was no acceleration of nerve repair on the eighth day after the injury. Level of Evidence I; High quality randomized clinical trial with or without statistically significant difference, but with narrow confidence intervals.
DOI: 10.6084/m9.figshare.10438232
title: Upregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in renal tissue in severe dengue in humans: Effects on endothelial activation/dysfunction

description: Abstract INTRODUCTION: Dengue is an important mosquito-borne disease in tropical and subtropical regions. Adhesion molecules have not been systematically characterized in the renal tissue of patients with severe dengue (SD). The objective of this study was to detect viral antigens in samples from patients that evolved with SD, correlating with the expression of ICAM-1, VCAM-1, VE-cadherin, and E-selectin to contribute to a better understanding of the pathophysiology of SD. METHODS: Kidney specimens from patients with SD were selected according to clinical and laboratorial data and submitted to histological and immunohistochemistry analysis. A semiquantitative evaluation was performed considering positive immunostaining in 20 glomeruli. RESULTS: Viral antigens were mainly detected in distal tubules. The intense immunostaining of VCAM-1 and ICAM-1 was observed. The expression of E-selectin was discrete, and VE-cadherin expression varied from mild to moderate. VCAM-1 was slightly intense in the glomerular capsule; the expression of ICAM-1 was diffuse. E-selectin was diffuse, and VE-cadherin varied from mild to moderate. The most frequent histological findings were glomerular congestion, mild glomerulitis, acute renal injury, and glomerular atrophy. CONCLUSIONS: The results appear to demonstrate an imbalance between vascular endothelial permeability regulating events in renal lesions in SD. The increase in the expression of ICAM-1 and VCAM-1 is an in-situ indicator of higher permeability with a consequent influx of cells favoring the inflammation of the endothelium. These molecules are important in the pathophysiology of the disease and provide the possibility of developing new markers for the evaluation, clinical follow-up, and therapeutic response of patients with SD.
DOI: 10.6084/m9.figshare.10438235.v1
title: Productive Performance, Egg Characteristics and Hatching Traits of Three Chicken Genotypes under Free-Range, Semi-Intensive, and Intensive Housing Systems

description: ABSTRACT The present study aimed at evaluating the effect of housing system on the live performance, egg quality, and hatching traits of three dual-purpose chicken genotypes. In total, 180 birds, comprising 48 pullets and 12 cockerels from each of three genotypes, were evaluated during the production phase (27-46 weeks). For this, 144 pullets and 36 cockerels were randomly picked from 18 treatment block groups were shifted to breeding coops, allotting 4 pullets to one cockerel. A completely randomized complete block design (RCBD) was employed. Three genotypes, purebred Naked Neck (NN) and two crosses Rhode Island Red × Naked Neck (RIR × NN = RNN) and Black Australorp × Naked Neck (BAL × NN = BNN), were compared. Intensive system (p&lt;0.0001) and BNN hens (p&lt;0.0001) were heavier on week 26 and 46. Higher egg production (p&lt;0.0001) was obtained in the intensive system and in BNN hens (p&lt;0.0001). Hens maintained in the intensive systems produced heavier eggs and higher egg mass (p&lt;0.0001), and RNN and BNN hens laid heavier eggs (p&lt;0.0001) while higher egg mass (p&lt;0.0001) was found in BNN hens. Higher egg shape index (initial, p=0.0002), egg surface area (initial, p&lt;0.0001; final, p&lt;0.0001), egg volume (initial, p&lt;0.0001; final, p&lt;0.0001) and Haugh unit score (initial, p=0.0002; final, p=&lt;0.0001) were obtained in RNN and BNN hens. At the end of the experiment (46 weeks), higher yolk index (p=0.0004) was found in RNN and BNN eggs, and thicker eggshells (p&lt;0.0001) in RNN eggs. Higher egg hatchability was obtained in the free-range system (p&lt;0.0001) and in the RNN genotype (p&lt;0.0001). The highest fertility rates were detected in the free-range system (p&lt;0.0001), and in the RNN and BNN genotypes (p&lt;0.0001). The lowest infertile egg rates were observed in the free-range system (p&lt;0.0001) and in RNN and BNN genotypes (p&lt;0.0001). The lowest dead-in-shell rate was calculated for the free-range system (p=0.0456). In conclusion, free-range and semi-intensive system largely influence productive performance, egg quality and hatching traits. Regarding genotypes, RNN and BNN crossbred hens perform better than NN purebreds.
DOI: 10.6084/m9.figshare.10438241.v1
title: A systematic scoping review of the genetic ancestry of the Brazilian population

description: Abstract The genetic background of the Brazilian population is mainly characterized by three parental populations: European, African, and Native American. The aim of this study was to overview the genetic ancestry estimates for different Brazilian geographic regions and analyze factors involved in these estimates. In this systematic scoping review were included 51 studies, comprehending 81 populations of 19 states from five regions of Brazil. To reduce the potential of bias from studies with different sampling methods, we calculated the mean genetic ancestry weighted by the number of individuals. The weighted mean proportions of European, African, and Native American ancestries were 68.1%, 19.6%, and 11.6%, respectively. At the regional level, the highest European contribution occurred in the South, while the highest African and Native American contributions occurred in the Northeastern and Northern regions, respectively. Among states in the Northeast region, Bahia and Ceará showed significant differences, suggesting distinct demographic histories. This review contributes for a broader understanding of the Brazilian ancestry and indicates that the ancestry estimates are influenced by the type of molecular marker and the sampling method.
DOI: 10.6084/m9.figshare.10438244.v1
title: Fruit quality of pineapple ‘Vitória’ under macronutrients and boron deficiency

description: Abstract The objective of this study was to evaluate the influence of macronutrient and boron deficiencies on physico-chemical characteristics of pineapple fruits, ‘Vitoria’ cultivar. The experimental design was a randomized complete block with eight treatments (complete solution, -N, -P, -K, -Ca, -Mg, -S and -B) and six replicates. The experiment was conducted from October 2016 to March 2018, and the variables evaluated were: fresh fruit and crown mass, fruit diameter, fruit and crown length, juice volume and mass, titratable acidity (TA), soluble solids (SS), vitamin C, juice pH and SS/TA ratio. All treatments with nutrient deficiency reduced the fresh fruit mass. In treatments -N and -Ca there was a reduction of 41.9 and 35.3%, respectively, in the crown fresh mass. Fruits diameter under N, K and Mg deficiency was lower, fruit length, soluble solids and juice pH were lower in treatment with N deficiency. Isolated deficiencies of each of the macronutrients and B reduced the mass of the whole fruit and the crownless fruit. The nutrient that most limited the size and quality of ‘Vitoria’ pineapple fruit was nitrogen.