cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.30496778
title: Development of preservice science teachers’ reflection skills: does reflecting on one’s own or other people’s instructional explanations lead to different developments?

description: Teachers’ reflection skills are crucial to their professional knowledge, yet research in this area, particularly among chemistry educators, remains sparse. Little is known about how teachers plan, teach, and reflect on science teaching in chemistry education. Also, promoting and developing reflection skills over more than three measurement points could be important for designing lessons and seminars. This research project will address these research gaps by developing a university seminar in which students plan, teach, observe, and then reflect on short teaching situations. The preservice science teachers reflected on the adaptive explanation of experiments, a chemistry specialty. Since both observed and self-conducted lessons can lead to higher reflection skills, differences in the development between these two conditions are investigated in an intervention study using a linear mixed model (LMM). The main results are that the developed university seminar can significantly promote the reflection skills of both Teachers and Observers. There are indications that the ability to reflect develops at different rates in the two groups. This study suggests that reflection abilities are related to professionalising individual sub-facets of preservice science teachers’ adaptive explaining skills in planning situations.
DOI: 10.6084/m9.figshare.30496778.v1
title: Development of preservice science teachers’ reflection skills: does reflecting on one’s own or other people’s instructional explanations lead to different developments?

description: Teachers’ reflection skills are crucial to their professional knowledge, yet research in this area, particularly among chemistry educators, remains sparse. Little is known about how teachers plan, teach, and reflect on science teaching in chemistry education. Also, promoting and developing reflection skills over more than three measurement points could be important for designing lessons and seminars. This research project will address these research gaps by developing a university seminar in which students plan, teach, observe, and then reflect on short teaching situations. The preservice science teachers reflected on the adaptive explanation of experiments, a chemistry specialty. Since both observed and self-conducted lessons can lead to higher reflection skills, differences in the development between these two conditions are investigated in an intervention study using a linear mixed model (LMM). The main results are that the developed university seminar can significantly promote the reflection skills of both Teachers and Observers. There are indications that the ability to reflect develops at different rates in the two groups. This study suggests that reflection abilities are related to professionalising individual sub-facets of preservice science teachers’ adaptive explaining skills in planning situations.
DOI: 10.6075/j0416vvv
title: CIL:39733. In Cell Image Library

description: EM images of chromatin fibers reconstituted from tandem arrays of a strong nucleosome positioning sequence flanked by 'linker' DNA, creating fibers containing 22 nucleosomes and a nucleosome repeat length (NRL) of 177 bp. Reconstitutes had saturating amounts of purified core histones and linker histones. (A) shows a montage of 'end on' views of fibers obtained by cryo-fixation and recorded in the frozen hydrated state. (B and C) contain views of 3D models considered to correspond to the images at left. See Fig 1 in P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.
description: See: P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.
description: Preparation: cryofixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image; portrayed image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution
Visualization methods: visualization of contiguous regions
Processing history: montage
Data qualification: Processed
DOI: 10.6075/j0r78d0p
title: CIL:39731, Gallus gallus gallus, erythrocyte. In Cell Image Library

description: TEM image of frozen hydrated soluble chromatin isolated from chicken erythrocyte nuclei and vitrified in low salt. Polynucleosomes adopt an irregular zigzag fiber conformation (a,b,k), and show a 'stem' motif. Similar features are seen in reconstituted polynucleosomes (c,d,f-i). However, if reconstitutes lack linker histone H5, the stem conformation is absent (c). 
 See Fig 1 in J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178.
description: Isolated nuclei were prepared from chicken erythrocyte nuclei and polynucleosomes released after treatment with micrococcal nuclease. Reconstituted polynucleosomes were prepared by salt dialysis using purified DNA and histones. Samples were fixed with 0.1% glutaraldehyde for 4 hr at 4C, placed on holey carbon films, vitrified by plunge freezing, and observed in the frozen hydrated state using a Philips CM10 TEM operated at 60KV. Low dose images were recorded on Kodak SO163 film.
See also: J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a un ique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178.
description: Preparation: glutaraldehyde fixed tissue; cryofixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution
Visualization methods: visualization of contiguous regions
Data qualification: Processed
DOI: 10.6075/j0jw8cng
title: CIL:39728. In Cell Image Library

description: Reconstitutes were fixed in the appropriate salt environment, applied to thin carbon films, air dried, and shadowed with Pt using electron bean evaporation (Balzers BA 080 vacuum evaporator. Grids were examined in a FEI Tecnai 12 TEM at 100KV with a LaB6 filament and tilted darkfield optics, and recorded using a TVIPS 2k CCD camera.
See also: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322.
description: TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were fixed with glutaraldehyde, applied to carbon films, and shadowed with Pt. (A, B) samples without linker histone H5 fixed in 5 mM NaCl; (C) Sample with H5, fixed in 150 mM NaCl; (D) sample with H5, fixed in 1 mM MgCl2.
 See Fig 1 in: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322
description: Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: CCD
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed
DOI: 10.6075/j0j67g72
title: CIL:39727, Mus musculus, cardiac muscle cell. In Cell Image Library

description: Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.
description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
description: Transmission electron micrograph of mouse cardiac muscle (ventricle). The image illustrates the myofibrils as well as a sarcomere in the area of fibrils between the two Z-lines. Z-lines link actin filaments of one sarcomere to the next, while the sarcomere is responsible for cardiac muscle contractions.
DOI: 10.6075/j0pk0fg5
title: CIL:39382, Amorphophallus titanum. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: visualization of contiguous regions; uranyl salt; gold
Processing history: unprocessed raw data
description: Transmission electron micrograph of an Amorphophallus titanum anther. This high magniification image shows pollen inside the locule (cavity where the pollen is located).This image is part of a group that includes CIL: 39377- 39384.
description: Images collected on a FEI XL-30 FEG ESEM. Sample preparation protocol is available on the Dartmouth EM Facility web site at http://remf.dartmouth.edu:8080/EM-Wiki/36.
DOI: 10.6075/j08w3cmn
title: CIL:39517, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Vorticella convallaria has a contractile stalk that arises from the aboral pole of the cell. The myonemes from the body converge at this pole and pass into the stalk to form a large contractile bundle called the spasmoneme. When contracted the stalk coils into a helix. The spasmoneme is partially encased in an extension of the pellicle and a thin layer of cytosol that is enclosed with plasma membrane. An additional secreted sheath surrounds the stalk. TEM taken on 4/6/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 7,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0xk8dxs
title: CIL:39504, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: The contractile vacuole of V. convallaria empties into a convoluted chamber. This chamber is covered by the alveoli and plasma membrane of the pellicle and is open to the cell‚Äôs surface via the peristome which is in turn connected to the perioral opening in the contracted cell. This preCV chamber apparently harbors (cultures?) its own flora of bacteria. Ribbons of microtubules link the CV pore to the CV. TEM taken on 3/29/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 11,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.13140/rg.2.2.18305.57449
title: TOURIST MAP OF KARBI ANGLONG
DOI: 10.6075/j0z89br9
title: CIL:39460, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Cytopharynx with numerous folded membrane ribs subtended by five or so microtubules. These microtubules have arms that bind to other microtubules and to the membrane of the cytopharynx and to surrounding vesicles of various sizes. The vesicles are uncoated and free of ribosomes. The vesicles are probably equivalent to the discoidal vesicles and/or to the 100nm vesicles; the latter arise from the endosomal pathway of Paramecium. Evidence for vesicle fusion to the cytopharynx membrane, between the ribs, can be seen. Bacteria and tips of cilia are present in the lumen of the cytopharynx. TEM taken on 4/6/72 by R. Allen with Hitachi HU11A operating at 75kV. Mag. 19,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
DOI: 10.6075/j00864nn
title: CIL:39456, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Tangentially sectioned dictyosomes that give rise to cupulas (see Favard, P. and N. Carasso, J. Microscopie 3:671-696, 1964) and coated vesicles. TEM taken on 4/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 24,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0fj2g4b
title: CIL:39454, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: A grazing section through the edge of the nuclear envelope. This illustrates nuclear envelope pores in face view. Polysomes, chains of ribosomes, are bound to the outer membrane of the nuclear envelope showing that this membrane is equivalent to rough ER as a site of protein synthesis for translocation across the membrane or for membrane protein synthesis. TEM taken on 2/5/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 28,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0w66k37
title: CIL:39453, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Only the macronucleus contains nucleoli which is the site of ribosomal RNA synthesis and ribosome subunit assembly. This EM also contains the distal end of the food vacuole forming cytopharynx which consists of a single membrane limited tube surrounded by microtubules that originate from the lamellae at the edge of the peristome. TEM taken on 3/29/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
DOI: 10.6075/j0319v65
title: CIL:39522, Saccharomyces cerevisiae. In Cell Image Library

description: Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements
description: The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.
description: A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc20, a cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C) required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate novel protein thought to be involved in cell cycle regulation.These phase,gfp z-series images are part of an image group that ranges from CIL:39521-39525. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
DOI: 10.6075/j0bg2nbg
title: CIL:39518, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

description: Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation
description: The basal bodies in the opisthe developing oral apparatus become organized into polykinetids of the three oral membranelles (1, 2 and 3) and of the two rows of basal bodies forming the undulating membrane. The outer row of basal bodies of the undulating membrane is already ciliated as are at least some of the polykinetids of the oral membranelles. Somatic cilia/basal body complexes composed of monokinetids are also present. TEM taken on 8/14/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
description: Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).