DOI: 10.5061/dryad.r7sqv9skt
title: Measures of male reproductive performance across mating events

description: The cost of reproduction is well studied in females but only recently have
 the costs of mating been investigated in males. Research suggests that
 males allocate resources between subsequent mating events, resulting in
 differential success across mating bouts. Selection should favor
 allocation strategies that match the likelihood of successive matings. The
 complexity of the system, however, suggests that one fixed strategy is
 unlikely to be universally favored and thus I predict that genetic
 variation for different allocation strategies will be segregating in
 natural populations. To test this, I measured several components of
 reproductive performance in eight inbred genotypes of Drosophila
 melanogaster across three sequential mating events. As predicted, there
 was genetic variation in how previous experience affected a male’s
 reproductive performance for both the proportion of matings that produced
 offspring and the proportion of offspring sired (P1). Some genotypes had
 the highest success in their first matings and declined in successive
 matings while other genotypes did best in later matings. Mating experience
 had consistent effects across genotypes on fertility and induced
 refractoriness to remating. On average, virgin matings produced the
 highest fertility, and third matings most effectively induced
 refractoriness. Genotype also had a significant effect on fertility. These
 results have important implications for understanding how selection may
 act on males when there is variation in the likelihood of multiple mating
 events and could affect the evolution of male allocation strategies in the
 face of perceived competitors.
description: # Measures of male reproductive performance across mating events
 [https://doi.org/10.5061/dryad.r7sqv9skt](https://doi.org/10.5061/dryad.r7sqv9skt) Anthony Fiumera: [afiumera@binghamton.edu](mailto:afiumera@binghamton.edu) ## Description of the data and file structure **"ExpMatters_R.csv"** * GENOTYPE: this is the DGRP Line that the male was from, and corresponds to the genotype in the manuscript * MATING_EXP: this is the mating experience for that particular male, FIRST, SECOND, THIRD * REP: the replicate, used to make the ID column * BLOCK: the block (generation) in which the flies were collected * ID: the actual individual male, used for repeated measures design * FEMALE: the genotype of the female (cnbw1 is recessive white-eyed) * V1W: number of white eyed offspring in Vial 1 (sired by competitor male) * V1R: number of red eyed offspring in Vial 1 (sired by focal male) * V2W: number of white eyed offspring in Vial 2 (sired by competitor male) * V2R: number of red eyed offspring in Vial 2 (sired by focal male) * SU1: set up time for the mating with the focal male (minutes past 0700hrs) * MATE1: Coded time interval mating with the focal male was observed (DNM is "did not mate") * MT1: time the mating was observed with the focal male (minutes past 0700hrs) * TTM1: Time to mating for the focal male, difference between SU1 and MT1 (this could produce negative values) * SU2: set up time for the mating with the competitor male (minutes past 0700hrs) * MATE2: Coded time interval mating with the competitor male was observed (DNM is "did not mate") * MT2: time the mating was observed with the competitor male (minutes past 0700hrs) * TTM2: Time to mating for the competitor male, difference between SU2 and MT2 (this could produce negative values) * RED_EYE: 1:0 coded for presence or absence of offspring with red eyes (sired by focal male) * TTM1_pos: Time to mating for the focal male, setting negative values to 0.5 or NA if exceed value indicate in paper * MATE1_EVER: 1:0 coded for whether the female mated to the focal male based on observations or offspring * TTM1_COX: time to mating for the focal male coded for right truncated Cox regression * RED_OFF: Total number of red eyed offspring (focal male), V1R + V2R * WHITE_EYE: 1:0 coded for whether there were any white eyed offspring (from competitor male) * TTM2_pos: Time to mating for the competitor male, setting negative values to 0.5 or NA if exceed value indicate in paper * MATE2_EVER: 1:0 coded for whether the female mated to the competitor male based on observations or offspring * TTM2_COX: time to mating for the competitor male coded for right truncated Cox regression ## Code/Software ExperienceMatters_Rscript.R R version 4.2.3 Required packages aod : version 1.3.3 car : version 3.1-2 coxme : version 2.2-18.1 dplyr : version 1.3.0 ggplot2 : version 3.4.4 lme4 : version 1.1-35.1 lmtest : version 0.9-40 miceadds : version 3.17-44 multcomp : version 1.4-25 robustlmm : version 3.3-1