cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14275196
title: Spring and summer ichthyoplankton assemblages in a temperate Patagonian gulf: an overview of temporal and spatial patterns on their structure

description: Abstract Knowing about the spatio-temporal patterns in the structure of ichthyoplankton assemblages allows inferring about the spawning behaviour of adult fishes, understanding the recruitment dynamics, and predicting the potential effects of mid- and long-term changes. Here, we studied the ichthyoplankton assemblages from the San José Gulf (Northern Patagonia, Argentina) and investigated their changes in space and time. To do that, we took monthly samples during two consecutive years, in spring and summer. A total of 2088 larvae were caught; they comprised 36 taxa, from which 14 were identified to species, two to genus, one to family and one to order. There were large differences in the structure of the assemblages between years, coincidently with marked changes in the surface water temperature. The structure of the ichthyoplankton assemblages also showed significant differences between the spring and summer: Helcogrammoides cunninghami, Dules auriga and larvae belonging to the family Engraulidae contributed most to these differences. The species diversity was higher in the colder year than in the warmer one. We discuss the potential role of environmental and oceanographic features on the interannual variability in the early stages of coastal fishes within a small gulf.
DOI: 10.6084/m9.figshare.14275197
title: Harvesting the cockle Leukoma pectorina (Lamarck, 1818) on Algodoal-Maiandeua Island (Pará, Brazil): techniques, bio-ecology, and ethnoecological knowledge

description: Abstract The ecological and ethnological aspects of the harvesting of the cockle, Leukoma pectorina (Bivalvia: Veneridae), were investigated on Algodoal-Maiandeua Island, on the Amazon coast of Brazil. Ethnobiological data were collected through informal conversations, semi-structured interviews, and observations of the harvesting and processing of bivalves on the island. Following the ethnobiology study, the cockle beds were surveyed to evaluate the density of L. pectorina, body size and the meat yield of the cockles in the months of dry and rainy seasons. In the study area, cockling is a manual and artisanal activity, and L. pectorina is typically harvested by mothers with little formal education. The cocklers make their own tools, cockles are prepared in the family environment, and the majority of the catch is sold to commercial establishments on the island. Cockling is a sporadic activity used to complement the family income, and is more common during the dry season, when tourism increases on Algodoal-Maiandeua Island. The cockles are also larger and population density is higher during this season, and the cocklers themselves recognize this period as providing the most productive harvest. These findings reinforce the value of traditional knowledge for both scientific research and the planning of the management of coastal fishery resources.
DOI: 10.6084/m9.figshare.14275197.v1
title: Harvesting the cockle Leukoma pectorina (Lamarck, 1818) on Algodoal-Maiandeua Island (Pará, Brazil): techniques, bio-ecology, and ethnoecological knowledge

description: Abstract The ecological and ethnological aspects of the harvesting of the cockle, Leukoma pectorina (Bivalvia: Veneridae), were investigated on Algodoal-Maiandeua Island, on the Amazon coast of Brazil. Ethnobiological data were collected through informal conversations, semi-structured interviews, and observations of the harvesting and processing of bivalves on the island. Following the ethnobiology study, the cockle beds were surveyed to evaluate the density of L. pectorina, body size and the meat yield of the cockles in the months of dry and rainy seasons. In the study area, cockling is a manual and artisanal activity, and L. pectorina is typically harvested by mothers with little formal education. The cocklers make their own tools, cockles are prepared in the family environment, and the majority of the catch is sold to commercial establishments on the island. Cockling is a sporadic activity used to complement the family income, and is more common during the dry season, when tourism increases on Algodoal-Maiandeua Island. The cockles are also larger and population density is higher during this season, and the cocklers themselves recognize this period as providing the most productive harvest. These findings reinforce the value of traditional knowledge for both scientific research and the planning of the management of coastal fishery resources.
DOI: 10.6084/m9.figshare.14275196.v1
title: Spring and summer ichthyoplankton assemblages in a temperate Patagonian gulf: an overview of temporal and spatial patterns on their structure

description: Abstract Knowing about the spatio-temporal patterns in the structure of ichthyoplankton assemblages allows inferring about the spawning behaviour of adult fishes, understanding the recruitment dynamics, and predicting the potential effects of mid- and long-term changes. Here, we studied the ichthyoplankton assemblages from the San José Gulf (Northern Patagonia, Argentina) and investigated their changes in space and time. To do that, we took monthly samples during two consecutive years, in spring and summer. A total of 2088 larvae were caught; they comprised 36 taxa, from which 14 were identified to species, two to genus, one to family and one to order. There were large differences in the structure of the assemblages between years, coincidently with marked changes in the surface water temperature. The structure of the ichthyoplankton assemblages also showed significant differences between the spring and summer: Helcogrammoides cunninghami, Dules auriga and larvae belonging to the family Engraulidae contributed most to these differences. The species diversity was higher in the colder year than in the warmer one. We discuss the potential role of environmental and oceanographic features on the interannual variability in the early stages of coastal fishes within a small gulf.
DOI: 10.6084/m9.figshare.14275199
title: Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/DAPI banding, and inferences about their karyotype differentiation

description: Abstract The species of the genus Arachis (Leguminosae) are ordered into nine sections. The assignment of genome types in this genus has been based on cross-compatibility analysis and molecular cytogenetic studies. The latter has also allowed karyotypically establishing well-defined genomes and reassigning the genome of several species. However, most of these studies have been focused mainly on the sections Arachis and Rhizomatosae. To increase the knowledge about the chromosome diversity of the whole genus, here we performed a detailed karyotype characterization of representative species of most of the sections and genomes of Arachis. This characterization included chromosome morphology, CMA/DAPI chromosome banding, and chromosome marker localization (rDNAloci and one satDNA sequence) by fluorescent in situ hybridization (FISH). Based on the data obtained and other previously published data, we established the karyotype similarities by cluster analysis and defined eleven karyotype groups. The grouping was partly coincident with the traditional genome assignment, except for some groups and some individual species. Karyotype similarities among some genomes were also found. The main characteristics of each karyotype group of Arachis were summarized. Together, our results provide information that may be beneficial for future cytogenetic and evolutionary studies, and also contribute to the identification of interspecific hybrids.
DOI: 10.6084/m9.figshare.14275199.v1
title: Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/DAPI banding, and inferences about their karyotype differentiation

description: Abstract The species of the genus Arachis (Leguminosae) are ordered into nine sections. The assignment of genome types in this genus has been based on cross-compatibility analysis and molecular cytogenetic studies. The latter has also allowed karyotypically establishing well-defined genomes and reassigning the genome of several species. However, most of these studies have been focused mainly on the sections Arachis and Rhizomatosae. To increase the knowledge about the chromosome diversity of the whole genus, here we performed a detailed karyotype characterization of representative species of most of the sections and genomes of Arachis. This characterization included chromosome morphology, CMA/DAPI chromosome banding, and chromosome marker localization (rDNAloci and one satDNA sequence) by fluorescent in situ hybridization (FISH). Based on the data obtained and other previously published data, we established the karyotype similarities by cluster analysis and defined eleven karyotype groups. The grouping was partly coincident with the traditional genome assignment, except for some groups and some individual species. Karyotype similarities among some genomes were also found. The main characteristics of each karyotype group of Arachis were summarized. Together, our results provide information that may be beneficial for future cytogenetic and evolutionary studies, and also contribute to the identification of interspecific hybrids.
DOI: 10.6084/m9.figshare.14275202
title: Evaluation of the antigenotoxic and antioxidant activity induced by Croton antisyphiliticus

description: Abstract This study aimed to investigate antigenotoxicity and antioxidant potential of extract, fractions and vitexin from C. antisyphiliticus. Methanolic extract was fractionated through solvents of increasing polarity. The composition of extracts and fractions were evaluated through phytochemical screening. Micronucleus test was performed in mice to evaluate the antigenotoxicity. Antioxidant activity was measured using the assay 1,1-diphenyl-2-picrylhydrazyl (DPPH), iron ion chelating, thiobarbituric acid assay and nitric oxide scavenging. Treatment with extract, fractions and vitexin did not produce an increase in Micronucleus mean values. However, Micronucleus (MN) mean values decreased in relation to control. methanolic extract presented antioxidant potential for DPPH (81%), iron ion chelating (77.8%), Thiobarbituric Acid (TBARS) (32.49%) and Nitric Oxide (NO) (80.97%). Ethyl acetate fraction showed the highest antioxidant activity (65.46%). The vitexin showed a Inhibitory Concentration (IC50) of DPPH value smaller in relation to control. Vitexin flavonoid was detected by High Performance Liquid Chromatography (HPLC), infrared spectrometry and nuclear magnetic resonance. It can be inferred that methanolic extract, fraction ethyl acetate and vitexin isolated from C. antisyphiliticus is endowed with antigenotoxic and antioxidant potential.
DOI: 10.6084/m9.figshare.14275202.v1
title: Evaluation of the antigenotoxic and antioxidant activity induced by Croton antisyphiliticus

description: Abstract This study aimed to investigate antigenotoxicity and antioxidant potential of extract, fractions and vitexin from C. antisyphiliticus. Methanolic extract was fractionated through solvents of increasing polarity. The composition of extracts and fractions were evaluated through phytochemical screening. Micronucleus test was performed in mice to evaluate the antigenotoxicity. Antioxidant activity was measured using the assay 1,1-diphenyl-2-picrylhydrazyl (DPPH), iron ion chelating, thiobarbituric acid assay and nitric oxide scavenging. Treatment with extract, fractions and vitexin did not produce an increase in Micronucleus mean values. However, Micronucleus (MN) mean values decreased in relation to control. methanolic extract presented antioxidant potential for DPPH (81%), iron ion chelating (77.8%), Thiobarbituric Acid (TBARS) (32.49%) and Nitric Oxide (NO) (80.97%). Ethyl acetate fraction showed the highest antioxidant activity (65.46%). The vitexin showed a Inhibitory Concentration (IC50) of DPPH value smaller in relation to control. Vitexin flavonoid was detected by High Performance Liquid Chromatography (HPLC), infrared spectrometry and nuclear magnetic resonance. It can be inferred that methanolic extract, fraction ethyl acetate and vitexin isolated from C. antisyphiliticus is endowed with antigenotoxic and antioxidant potential.
DOI: 10.6084/m9.figshare.14275203
title: Reproductive biology of Erythrolamprus jaegeri coralliventris (Serpentes: Dipsadidae) in the Brazilian Coastal Pampa

description: Abstract We analyzed reproductive biology of Erythrolamprus jaegeri coralliventris, a snake from the Brazilian Pampa. Females presented larger snout-vent length than males, while no significant differences were found in tail length/snout-vent length ratios between sexes. Females attain sexual maturity in larger sizes than males. The reproductive cycle of females presented a seasonal pattern, with advanced vitellogenesis occurring from middle winter to middle spring and oviductal eggs occurring from middle winter to middle summer. The real fecundity ranged from two to eigth eggs and the expected fecundity varied from one to 12 secondary follicles. No significant correlation was found between females body size and the following parameters: real fecundity, length of the largest egg and potential fecundity. Therefore, E. j. coralliventris presents a seasonal reproductive pattern, which seems to follow the rainfall profile observed for the studied region. This may represent a strategy of energy gain associated to the reproductive cycle, considering that the food resources most explored by this species are anurans that present higher activity during rainy periods.
DOI: 10.6084/m9.figshare.14275205
title: Bryophyte flora of two Natural Parks in Amapá: richness, composition and new records

description: Abstract The aim of this study is to investigate the richness and composition of the bryophytes of two Municipal Natural Parks in Amapá, eastern Amazon. Bryophytes were collected in the Arivaldo Gomes Barreto Municipal Natural Park (AGBMNP), Macapá municipality, and Cancão Municipal Natural Park (CMNP), Serra do Navio municipality, in October 2010 and October 2012, respectively. Ten plots of 10 x 10 m were established in each park for sampling, and, in addition, random collections were made, in order to maximize the knowledge on the local bryophyte flora. In total, 53 species were identified in AGBMNP, and 110 species in CMNP. The results shown a lower richness in AGBMNP than in CMNP, which may be related to the own size of each park, the level of conservation, and its localization. In terms of abundance, the neotropical pattern and the species locally rare were most representative in both parks, however, the floristic composition diverged. The AGBMNP is composed mostly by generalist species, while the CMNP by both generalists and shade specialists. The present study recorded 54% of the known bryophyte flora of Amapá, 63 new records for the state and, in addition, three new records for the North region of Brazil.
DOI: 10.6084/m9.figshare.14275205.v1
title: Bryophyte flora of two Natural Parks in Amapá: richness, composition and new records

description: Abstract The aim of this study is to investigate the richness and composition of the bryophytes of two Municipal Natural Parks in Amapá, eastern Amazon. Bryophytes were collected in the Arivaldo Gomes Barreto Municipal Natural Park (AGBMNP), Macapá municipality, and Cancão Municipal Natural Park (CMNP), Serra do Navio municipality, in October 2010 and October 2012, respectively. Ten plots of 10 x 10 m were established in each park for sampling, and, in addition, random collections were made, in order to maximize the knowledge on the local bryophyte flora. In total, 53 species were identified in AGBMNP, and 110 species in CMNP. The results shown a lower richness in AGBMNP than in CMNP, which may be related to the own size of each park, the level of conservation, and its localization. In terms of abundance, the neotropical pattern and the species locally rare were most representative in both parks, however, the floristic composition diverged. The AGBMNP is composed mostly by generalist species, while the CMNP by both generalists and shade specialists. The present study recorded 54% of the known bryophyte flora of Amapá, 63 new records for the state and, in addition, three new records for the North region of Brazil.
DOI: 10.6084/m9.figshare.14275206
title: Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations

description: Abstract We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different configurations: static flat-bed, plugged-flow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defined as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 oC, in static flat-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-flow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterification and transesterification reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst.
DOI: 10.6084/m9.figshare.14275206.v1
title: Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations

description: Abstract We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different configurations: static flat-bed, plugged-flow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defined as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 oC, in static flat-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-flow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterification and transesterification reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst.
DOI: 10.6084/m9.figshare.14275208
title: Bacteriocins as an alternative in the treatment of infections by Staphylococcus aureus

description: Abstract Staphylococcus aureus (S. aureus) is a highly versatile Gram-positive bacterium that is carried asymptomatically by up to 30% of healthy people, while being a major cause of healthcare-associated infections, making it a worldwide problem in clinical medicine. The adaptive evolution of S. aureus strains is demonstrated by its remarkable capacity to promptly develop high resistance to multiple antibiotics, thus limiting treatment choice. Nowadays, there is a continuous demand for an alternative to the use of antibiotics for S. aureus infections and a strategy to control the spread or to kill phylogenetically related strains. In this scenario, bacteriocins fit as with a promising and interesting alternative. These molecules are produced by a range of bacteria, defined as ribosomally synthesized peptides with bacteriostatic or bactericidal activity against a wide range of pathogens. This work reviews ascertained the main antibiotic-resistance mechanisms of S. aureus strains and the current, informative content concerning the applicability of the use of bacteriocins overlapping the use of conventional antibiotics in the context of S. aureus infections. Besides, we highlight the possible application of these biomolecules on an industrial scale in future work.
DOI: 10.6084/m9.figshare.14275208.v1
title: Bacteriocins as an alternative in the treatment of infections by Staphylococcus aureus

description: Abstract Staphylococcus aureus (S. aureus) is a highly versatile Gram-positive bacterium that is carried asymptomatically by up to 30% of healthy people, while being a major cause of healthcare-associated infections, making it a worldwide problem in clinical medicine. The adaptive evolution of S. aureus strains is demonstrated by its remarkable capacity to promptly develop high resistance to multiple antibiotics, thus limiting treatment choice. Nowadays, there is a continuous demand for an alternative to the use of antibiotics for S. aureus infections and a strategy to control the spread or to kill phylogenetically related strains. In this scenario, bacteriocins fit as with a promising and interesting alternative. These molecules are produced by a range of bacteria, defined as ribosomally synthesized peptides with bacteriostatic or bactericidal activity against a wide range of pathogens. This work reviews ascertained the main antibiotic-resistance mechanisms of S. aureus strains and the current, informative content concerning the applicability of the use of bacteriocins overlapping the use of conventional antibiotics in the context of S. aureus infections. Besides, we highlight the possible application of these biomolecules on an industrial scale in future work.
DOI: 10.6084/m9.figshare.14275209
title: Effect of dietary NiCl2 on the cell cycle of cecal tonsil in the chicken broiler

description: Abstract Although the effects of nickel chloride (NiCl2) on the immune system have long been recognized, little is known about the effects of nickel (II) on the cell cycle and related signaling events in immune organs, such as cecal tonsil, a key immune organ of chicken. In the present study, we investigated the effect of NiCl2 on the cell cycle of cecal tonsil. The cell cycle was detected by the methods of flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). The results showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase of the cecal tonsil. The G2/M cell cycle arrest was accompanied by the up-regulation of p53, p21 protein expression and mRNA expression, and down-regulation of cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and mRNA expression. The data suggested that the cells’ (mainly the T lymphocytes) proliferation in the cecal tonsil was inhibited by the high dietary NiCl2.