cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.26680106
title: Additional file 3 of Analysis of forage quality, volatile organic compounds and metabolic pathways in alfalfa (Medicago sativa L.) at different stages based on electronic nose and GC-MS

description: Additional file 3: Table S2. Different volatile organic compounds and odor characteristics.
DOI: 10.15468/dl.9uqmfb
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "Geometry" : [
 "POLYGON ((149.070191 -32.663433, 149.070191 -32.676907, 149.079174 -32.676907, 149.079174 -32.667924, 149.074683 -32.667924, 149.074683 -32.663433, 149.070191 -32.663433))"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067329-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.nat8yj
title: Occurrence Download

description: A dataset containing 10628496 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is Chile",
 "Geometry POLYGON((-147.02116 -18.34508,-149.63797 -44.07555,-91.66443 -65.46201,-66.09067 -59.16857,-64.10571 -14.87553,-147.02116 -18.34508))",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present"
 ]
}

 The dataset includes 10628496 records from 1326 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0067319-240626123714530/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/g1pezi
title: 59161.pdf

description: :unav
DOI: 10.48668/ukhbsu/irsohz
title: 61529.pdf

description: :unav
DOI: 10.48668/ukhbsu/5ykvcu
title: 59605.pdf

description: :unav
DOI: 10.48668/ukhbsu/c8vc2k
title: 60549.pdf

description: :unav
DOI: 10.48668/ukhbsu/njj78s
title: 59609.pdf

description: :unav
DOI: 10.48668/ukhbsu/oltmko
title: 59453.pdf

description: :unav
DOI: 10.48668/ukhbsu/izcanw
title: 58827.pdf

description: :unav
DOI: 10.48668/ukhbsu/1pono9
title: 59452.pdf

description: :unav
DOI: 10.48668/ukhbsu/mt9v8d
title: 61356.pdf

description: :unav
DOI: 10.48668/ukhbsu/ml4lbt
title: 60455.pdf

description: :unav
DOI: 10.48668/ukhbsu/rod2zi
title: 61164.pdf

description: :unav
DOI: 10.48668/ukhbsu/1iuul1
title: 60589.pdf

description: :unav
DOI: 10.48668/ukhbsu/bt5hhe
title: 58900.pdf

description: :unav
DOI: 10.48668/ukhbsu/m2vtho
title: 60316.pdf

description: :unav
DOI: 10.48668/ukhbsu/48qzco
title: 59711.pdf

description: :unav
DOI: 10.48668/ukhbsu/sg2kw0
title: 59428.pdf

description: :unav
DOI: 10.48668/ukhbsu/e4j50q
title: 60965.pdf

description: :unav
DOI: 10.48668/ukhbsu/2goxbv
title: 58748.pdf

description: :unav
DOI: 10.48668/ukhbsu/lzdhxi
title: 61414.pdf

description: :unav
DOI: 10.48668/ukhbsu/hzv6sf
title: 60342.pdf

description: :unav
DOI: 10.48668/ukhbsu/2ihv0w
title: 60557.pdf

description: :unav
DOI: 10.48668/ukhbsu/cgn7he
title: 59586.pdf

description: :unav
DOI: 10.48668/ukhbsu/wifchs
title: 60080.pdf

description: :unav
DOI: 10.48668/ukhbsu/ohw5tj
title: 60373.pdf

description: :unav
DOI: 10.48668/ukhbsu/4cdhjd
title: 59707.pdf

description: :unav
DOI: 10.48668/ukhbsu/ysoncj
title: 60713.pdf

description: :unav
DOI: 10.48668/ukhbsu/pkrdjd
title: 60279.pdf

description: :unav
DOI: 10.48668/ukhbsu/wiceja
title: 58482.pdf

description: :unav
DOI: 10.48668/ukhbsu/jcpt5e
title: 58375.pdf

description: :unav
DOI: 10.48668/ukhbsu/9rmvpl
title: 59721.pdf

description: :unav
DOI: 10.48668/ukhbsu/gv8mez
title: 60588.pdf

description: :unav
DOI: 10.48668/ukhbsu/y5pnta
title: 58631.pdf

description: :unav
DOI: 10.15468/dl.mh5gwp
title: Occurrence Download

description: A dataset containing 722 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pteris fauriei",
 "Geometry POLYGON((119.300000 20.500000, 122.100000 20.500000, 122.100000 25.400000, 119.300000 25.400000, 119.300000 20.500000))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 722 records from 12 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028579-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/2wybjd
title: 57876.pdf

description: :unav
DOI: 10.48668/ukhbsu/dd7wss
title: 57967.pdf

description: :unav
DOI: 10.48668/ukhbsu/xytvnb
title: 61275.pdf

description: :unav
DOI: 10.48668/ukhbsu/8vrb0u
title: 64941.pdf

description: :unav
DOI: 10.48668/ukhbsu/jyhjuy
title: 62594.pdf

description: :unav
DOI: 10.48668/ukhbsu/udxt5l
title: 62380.pdf

description: :unav
DOI: 10.15468/dl.kverww
title: Occurrence Download

description: A dataset containing 15 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Melilotus altissimus",
 "Country is Ireland",
 "HasCoordinate is true"
 ]
}

 The dataset includes 15 records from 2 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028581-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/xukokd
title: 64256.pdf

description: :unav
DOI: 10.48668/ukhbsu/tnzjdj
title: 64117.pdf

description: :unav
DOI: 10.15468/dl.uwhztu
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Melilotus altissimus",
 "Country is Japan",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028582-250920141307145/datasets/export for details.
DOI: 10.48668/ukhbsu/gw0zfy
title: 61814.pdf

description: :unav
DOI: 10.48668/ukhbsu/o7juyz
title: 63048.pdf

description: :unav
DOI: 10.48668/ukhbsu/zbmoyz
title: 61906.pdf

description: :unav
DOI: 10.48668/ukhbsu/tfvd60
title: 64012.pdf

description: :unav
DOI: 10.15468/dl.mxdbuk
title: Occurrence Download

description: A dataset containing 17 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Melilotus altissimus",
 "Country is Canada",
 "HasCoordinate is true"
 ]
}

 The dataset includes 17 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028580-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/nf53fh
title: 63723.pdf

description: :unav
DOI: 10.48668/ukhbsu/ullu5c
title: 61736.pdf

description: :unav
DOI: 10.48668/ukhbsu/no53jb
title: 62056.pdf

description: :unav
DOI: 10.48668/ukhbsu/nkxpp6
title: 61629.pdf

description: :unav
DOI: 10.48668/ukhbsu/rpdv4g
title: 61945.pdf

description: :unav
DOI: 10.48668/ukhbsu/rnp8s4
title: 62777.pdf

description: :unav
DOI: 10.48668/ukhbsu/ugggkq
title: 63198.pdf

description: :unav
DOI: 10.48668/ukhbsu/6k8jfu
title: 62870.pdf

description: :unav
DOI: 10.48668/ukhbsu/0lraaa
title: 62208.pdf

description: :unav
DOI: 10.48668/ukhbsu/damqx9
title: 63966.pdf

description: :unav
DOI: 10.48668/ukhbsu/wmollg
title: 61648.pdf

description: :unav
DOI: 10.48668/ukhbsu/spjopt
title: 63324.pdf

description: :unav
DOI: 10.15468/dl.naq6ut
title: Occurrence Download

description: A dataset containing 183 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Geometry POLYGON((85.53377 -10.41037,172.37474 -10.41037,172.37474 24.31115,85.53377 24.31115,85.53377 -10.41037))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Amblyomma helvolum"
 ]
}

 The dataset includes 183 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028583-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/riqaw4
title: 61685.pdf

description: :unav
DOI: 10.48668/ukhbsu/9ldb04
title: 63874.pdf

description: :unav
DOI: 10.48668/ukhbsu/icquen
title: 62766.pdf

description: :unav
DOI: 10.48668/ukhbsu/ewdj6x
title: 63692.pdf

description: :unav
DOI: 10.48668/ukhbsu/7tunkb
title: 63873.pdf

description: :unav
DOI: 10.48668/ukhbsu/ugqkfs
title: 62617.pdf

description: :unav
DOI: 10.48668/ukhbsu/y8hdx1
title: 63718.pdf

description: :unav
DOI: 10.48668/ukhbsu/6eqmcd
title: 61860.pdf

description: :unav
DOI: 10.48668/ukhbsu/fm6zgk
title: 64603.pdf

description: :unav
DOI: 10.48668/ukhbsu/3g9tth
title: 61770.pdf

description: :unav
DOI: 10.48668/ukhbsu/ra0iis
title: 62382.pdf

description: :unav
DOI: 10.48668/ukhbsu/3jr47q
title: 63051.pdf

description: :unav
DOI: 10.48668/ukhbsu/qjtbxa
title: 62609.pdf

description: :unav
DOI: 10.48668/ukhbsu/4o3tpz
title: 61772.pdf

description: :unav
DOI: 10.48668/ukhbsu/vked2t
title: 64022.pdf

description: :unav
DOI: 10.48668/ukhbsu/w0rvmb
title: 61669.pdf

description: :unav
DOI: 10.48668/ukhbsu/7t2ckh
title: 62047.pdf

description: :unav
DOI: 10.48668/ukhbsu/bwnhxl
title: 63215.pdf

description: :unav
DOI: 10.48668/ukhbsu/k1nkf3
title: 63755.pdf

description: :unav
DOI: 10.48668/ukhbsu/8hfehr
title: 61636.pdf

description: :unav
DOI: 10.48668/ukhbsu/pks5us
title: 62706.pdf

description: :unav
DOI: 10.48668/ukhbsu/bbbocw
title: 62195.pdf

description: :unav
DOI: 10.48668/ukhbsu/vbykrv
title: 63066.pdf

description: :unav
DOI: 10.48668/ukhbsu/vkpxda
title: 64419.pdf

description: :unav
DOI: 10.48668/ukhbsu/kejotx
title: 62012.pdf

description: :unav
DOI: 10.48668/ukhbsu/i3rr45
title: 63106.pdf

description: :unav
DOI: 10.48668/ukhbsu/mshccg
title: 61691.pdf

description: :unav
DOI: 10.48668/ukhbsu/ye4u3b
title: 63421.pdf

description: :unav
DOI: 10.48668/ukhbsu/y5icsd
title: 64931.pdf

description: :unav
DOI: 10.48668/ukhbsu/7kzsng
title: 68079.pdf

description: :unav
DOI: 10.48668/ukhbsu/vtht20
title: 68176.pdf

description: :unav
DOI: 10.48668/ukhbsu/yemf7a
title: 65853.pdf

description: :unav
DOI: 10.15468/dl.u4ca3z
title: Occurrence Download

description: A dataset containing 22690 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ludwigia grandiflora)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 22690 records from 410 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028586-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/mvk4iu
title: 69265.pdf

description: :unav
DOI: 10.48668/ukhbsu/cr47va
title: 66573.pdf

description: :unav
DOI: 10.48668/ukhbsu/zhfphp
title: 65198.pdf

description: :unav
DOI: 10.15468/dl.3mm8fr
title: Occurrence Download

description: A dataset containing 601 species occurrences available in GBIF matching the query:
{
 "and" : [
 "Country is one of (China, Mongolia)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "OccurrenceStatus is Present",
 "TaxonKey is Potentilla fragarioides",
 "Year 1000-2025"
 ]
}

 The dataset includes 601 records from 24 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028588-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/e1ov6q
title: 66451.pdf

description: :unav
DOI: 10.48668/ukhbsu/wdhdty
title: 68497.pdf

description: :unav
DOI: 10.48668/ukhbsu/ljwksh
title: 65482.pdf

description: :unav
DOI: 10.48668/ukhbsu/0mvees
title: 68259.pdf

description: :unav
DOI: 10.48668/ukhbsu/s0vsay
title: 65834.pdf

description: :unav
DOI: 10.48668/ukhbsu/isxmtx
title: 67234.pdf

description: :unav
DOI: 10.48668/ukhbsu/jeonrq
title: 69487.pdf

description: :unav
DOI: 10.48668/ukhbsu/rvqpwk
title: 68073.pdf

description: :unav
DOI: 10.48668/ukhbsu/aamxfq
title: 69098.pdf

description: :unav
DOI: 10.48668/ukhbsu/bq5mbd
title: 65226.pdf

description: :unav
DOI: 10.48668/ukhbsu/0c28w4
title: 68000.pdf

description: :unav
DOI: 10.48668/ukhbsu/mgnu7t
title: 66551.pdf

description: :unav
DOI: 10.15468/dl.npy5us
title: Occurrence Download

description: A dataset containing 52312 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Gambusia affinis)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 52312 records from 131 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028557-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/gmuinz
title: 68781.pdf

description: :unav
DOI: 10.48668/ukhbsu/xamgbq
title: 69093.pdf

description: :unav
DOI: 10.48668/ukhbsu/hjyxx8
title: 65272.pdf

description: :unav
DOI: 10.48668/ukhbsu/glx1cq
title: 67435.pdf

description: :unav
DOI: 10.48668/ukhbsu/6nxdmd
title: 68252.pdf

description: :unav
DOI: 10.15468/dl.sntwa4
title: Occurrence Download

description: A dataset containing 160 species occurrences available in GBIF matching the query:
{
 "TaxonKey" : [
 "is Amblyomma javanense"
 ]
}

 The dataset includes 160 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028590-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/iwueqv
title: 66027.pdf

description: :unav
DOI: 10.48668/ukhbsu/okepdz
title: 70437.pdf

description: :unav
DOI: 10.15468/dl.q3vhsw
title: Occurrence Download

description: A dataset containing 1555 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is one of (Ovis gmelini)",
 "BasisOfRecord is one of (Specimen, Literature Occurrence, Human Observation, Observation)",
 "HasCoordinate is true",
 "HasGeospatialIssue is false",
 "Year is greater than or equal to 1991",
 "Year is less than or equal to 2025"
 ]
}

 The dataset includes 1555 records from 7 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028587-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.48668/ukhbsu/wy15uz
title: 68082.pdf

description: :unav
DOI: 10.48668/ukhbsu/zu1mqf
title: 70401.pdf

description: :unav
DOI: 10.48668/ukhbsu/yf1ygq
title: 68258.pdf

description: :unav
DOI: 10.6084/m9.figshare.29323367
title: Data for: Do Asian elephants plan for mutually-exclusive outcomes?

description: Data from the publication: “Do Asian elephants plan for mutually-exclusive outcomes?”. Please refer to the manuscript for descriptions of the methods used to produce these data and the statistical analyses used to analyze these data.There are four data sheets:individual_data.csv – this includes all data from all individuals tested individually and in pairs. Each row represents one trial of one individual. If elephants were tested in pairs, there are two rows for each trial (i.e., one row for the behavior of each individual).pair_data.csv – this includes data that are specific to pair trials. Each row is one pair trial; there are not two rows for each trial, but rather, pairs are analyzed as a unit.num_switch_data.csv – this includes data from each set (i.e., 1 set = 12 trials). Each row represents one set of one individual. If elephants were tested in pairs, there are two rows for each set (i.e., one row for the behavior of each individual).nammei_only.csv – this datasheet is similar to ‘individual_data.csv’ but only includes data from one individual (Nammei). There are also extra columns (behaviors) in this datasheet that are not included in ‘individual_data.csv’.Please see the readme file for descriptions of column names for all data sheets.Statistical analyses can be accessed in the "script.R" file.
DOI: 10.6084/m9.figshare.29323367.v1
title: Data for: Do Asian elephants plan for mutually-exclusive outcomes?

description: Data from the publication: “Do Asian elephants plan for mutually-exclusive outcomes?”. Please refer to the manuscript for descriptions of the methods used to produce these data and the statistical analyses used to analyze these data.There are four data sheets:individual_data.csv – this includes all data from all individuals tested individually and in pairs. Each row represents one trial of one individual. If elephants were tested in pairs, there are two rows for each trial (i.e., one row for the behavior of each individual).pair_data.csv – this includes data that are specific to pair trials. Each row is one pair trial; there are not two rows for each trial, but rather, pairs are analyzed as a unit.num_switch_data.csv – this includes data from each set (i.e., 1 set = 12 trials). Each row represents one set of one individual. If elephants were tested in pairs, there are two rows for each set (i.e., one row for the behavior of each individual).nammei_only.csv – this datasheet is similar to ‘individual_data.csv’ but only includes data from one individual (Nammei). There are also extra columns (behaviors) in this datasheet that are not included in ‘individual_data.csv’.Please see the readme file for descriptions of column names for all data sheets.Statistical analyses can be accessed in the "script.R" file.
DOI: 10.48668/ukhbsu/4ztjaq
title: 66239.pdf

description: :unav
DOI: 10.48668/ukhbsu/vgwczk
title: 67249.pdf

description: :unav
DOI: 10.48668/ukhbsu/gf7t0p
title: 68017.pdf

description: :unav
DOI: 10.15468/dl.g93tqd
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Paradoxornis aemodius",
 "Country is one of (Brunei Darussalam, Indonesia, Philippines, Timor-Leste, Singapore)",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028589-250920141307145/datasets/export for details.
DOI: 10.48668/ukhbsu/btnxva
title: 65248.pdf

description: :unav
DOI: 10.48668/ukhbsu/mvctex
title: 65547.pdf

description: :unav
DOI: 10.48668/ukhbsu/9ulqfc
title: 65118.pdf

description: :unav
DOI: 10.48668/ukhbsu/plkenh
title: 65141.pdf

description: :unav
DOI: 10.48668/ukhbsu/ldrtnu
title: 69812.pdf

description: :unav
DOI: 10.48668/ukhbsu/q2sstt
title: 66078.pdf

description: :unav
DOI: 10.48668/ukhbsu/1ozojd
title: 70468.pdf

description: :unav
DOI: 10.48668/ukhbsu/qhg099
title: 66615.pdf

description: :unav
DOI: 10.48668/ukhbsu/iehkey
title: 67442.pdf

description: :unav
DOI: 10.48668/ukhbsu/etpinr
title: 68382.pdf

description: :unav
DOI: 10.15468/dl.jad7s2
title: Occurrence Download

description: A dataset containing 301 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Pteris grevilleana",
 "Geometry POLYGON((119.300000 20.500000, 122.100000 20.500000, 122.100000 25.400000, 119.300000 25.400000, 119.300000 20.500000))",
 "HasCoordinate is true",
 "HasGeospatialIssue is false"
 ]
}

 The dataset includes 301 records from 13 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028593-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.rgdq93
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Melilotus altissimus",
 "Country is Latvia",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028594-250920141307145/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.15468/dl.gayunk
title: Occurrence Download

description: A dataset containing 0 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Melilotus altissimus",
 "Country is Lithuania",
 "HasCoordinate is true"
 ]
}

 The dataset includes 0 records from 0 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0028595-250920141307145/datasets/export for details.
DOI: 10.5281/zenodo.17223100
title: Introducing NOMAD: A Field-Deployable Workflow for Rapid, On-Site Microbiome Analysis of Raw Bovine Milk Using Full-Length 16S rRNA Sequencing (Supplementary Files)

description: Raw bovine milk hosts a diverse microbiota that profoundly influences dairy product quality, safety, and shelf-life. However, current surveillance methods are time-intensive and often lack the taxonomic resolution needed for effective risk mitigation. To address this, we developed NOMAD (Nanopore-based On-site Microbiome Analysis of Diversity), a field-deployable workflow for rapid, high-resolution characterization of the raw milk microbiome using full-length 16S rRNA gene sequencing via Oxford Nanopore Technologies. Milk samples collected from a commercial dairy operation were processed using eight DNA extraction protocols, with Method 4—incorporating EDTA and TE buffer—emerging as the optimal approach for microbial richness and DNA yield. Sequencing was performed on a MinION Mk1B platform, and bioinformatic analyses revealed that a 4-hour run was sufficient to recover >90% of total community richness, with stable alpha and beta diversity metrics by this timepoint. The complete workflow, including DNA extraction, library preparation, sequencing, and analysis, was completed in 10.5 hours, enabling same-day microbiome profiling in farm-adjacent settings. Comparative analysis showed strong agreement with established milk microbiome studies, while full-length reads enhanced resolution of spoilage-associated taxa such as Pseudomonas spp. and Streptococcus spp. The NOMAD platform offers a powerful and practical tool for near real-time microbiological surveillance in the dairy industry, supporting proactive quality control and improved food safety outcomes.
DOI: 10.5281/zenodo.17223101
title: Introducing NOMAD: A Field-Deployable Workflow for Rapid, On-Site Microbiome Analysis of Raw Bovine Milk Using Full-Length 16S rRNA Sequencing (Supplementary Files)

description: Raw bovine milk hosts a diverse microbiota that profoundly influences dairy product quality, safety, and shelf-life. However, current surveillance methods are time-intensive and often lack the taxonomic resolution needed for effective risk mitigation. To address this, we developed NOMAD (Nanopore-based On-site Microbiome Analysis of Diversity), a field-deployable workflow for rapid, high-resolution characterization of the raw milk microbiome using full-length 16S rRNA gene sequencing via Oxford Nanopore Technologies. Milk samples collected from a commercial dairy operation were processed using eight DNA extraction protocols, with Method 4—incorporating EDTA and TE buffer—emerging as the optimal approach for microbial richness and DNA yield. Sequencing was performed on a MinION Mk1B platform, and bioinformatic analyses revealed that a 4-hour run was sufficient to recover >90% of total community richness, with stable alpha and beta diversity metrics by this timepoint. The complete workflow, including DNA extraction, library preparation, sequencing, and analysis, was completed in 10.5 hours, enabling same-day microbiome profiling in farm-adjacent settings. Comparative analysis showed strong agreement with established milk microbiome studies, while full-length reads enhanced resolution of spoilage-associated taxa such as Pseudomonas spp. and Streptococcus spp. The NOMAD platform offers a powerful and practical tool for near real-time microbiological surveillance in the dairy industry, supporting proactive quality control and improved food safety outcomes.