DOI: 10.5281/zenodo.4818524
title: Morphometric data from: Two species? - Limits of the species concepts in the pygmy grasshoppers of the Tetrix bipunctata complex (Orthoptera: Tetrigidae)

description: Here we provide the complete set of files used by Moser et al., (2021, see References section below for the complete citation) for the morphometric analysis. In particular, we provide the following documents: - ANOVA_Tetrix_bipunctata_complex.R: R-script for calculating the ANOVA. You may get the necessary MRA source script from http://doi.org/10.5281/zenodo.4250142<br> <br> - Appendix_02_Table_Textrix_bipunctata_complex_summary_statistics.csv: CSV table of basic summary statistics (minimum, mean etc.) of body measurements<br> <br> - Appendix_02_Table_Textrix_bipunctata_complex_summary_statistics.xlsx: Excel table of basic summary statistics (minimum, mean etc.) of body measurements<br> <br> - Appendix_02_Tetrix_bipunctata_complex_summary_statistics.R: R-script for calculating basic summary statistics (minimum, mean etc.) of body measurements.<br> <br> - Data_Tetrix_bipunctata_complex.csv: Dataset with raw values (in millimeters) of all 273 specimens. NAs replaced using the R-script "Data_Tetrix_bipunctata_complex_multiple_imputation_mice.R" below. This is the datafile used for all morphometric analyses. - Data_Tetrix_bipunctata_complex_multiple_imputation_mice.R: R-script used for replacing NAs. - Data_Tetrix_bipunctata_complex_rawdata_with_NA.csv: Dataset with raw values (in millimeters) of all 273 specimens. NAs not replaced.<br> <br> - Figure_02_Tetrix_bipunctata_complex_shape_PCA.R: R-script for calculating the shape PCA (Fig. 2A) and PCA ratio spectra (Figs. 2B, 2C) (download Fig. 2 from publisher). You may get the necessary MRA source script from http://doi.org/10.5281/zenodo.4250142<br> <br> - Figure_03_Tetrix_bipunctata_complex_allometry.R: R-script for plotting the isometric size against shape PCA for checking allometry (download Fig. 3 from publisher). You may get the necessary MRA source script from http://doi.org/10.5281/zenodo.4250142<br> <br> - Figure_04_Tetrix_bipunctata_complex_ratios.R: R-script for calculating boxplots of best ratios (Figs. 4A, 4C) and the standard ratio (Fig. 4B) (download Fig. 4 from publisher). You may get the necessary MRA source scripts from http://doi.org/10.5281/zenodo.4250142<br> <br> - Figure_05_Tetrix_bipunctata_complex_intermediates.R: R-script for plotting the isometric size against the best (Fig. 5A) and the standard ratio (Fig. 5B) for checking the position of Nadig's intermediate specimens (download Fig. 5 from publisher). You may get the necessary MRA source script from http://doi.org/10.5281/zenodo.4250142<br> <br> - IamgeJ_script_100x.txt: ImageJ script used for taking measurements at 100x magnification<br> <br> - IamgeJ_script_150x.txt: ImageJ script used for taking measurements at 150x magnification<br> <br> - IamgeJ_script_30x.txt: ImageJ script used for taking measurements at 30x magnification<br> <br> - scale_100x.tif: photo of scale taken with Keyence VHX 2000 digital microscope at 100x magnification<br> <br> - scale_150x.tif: photo of scale taken with Keyence VHX 2000 digital microscope at 150x magnification<br> <br> - scale_30x.tif: photo of scale taken with Keyence VHX 2000 digital microscope at 30x magnification<br> <br> - Supplementary_Figure_01_Tetrix_bipunctata_complex_character_reliability.R: R-script for calculating the shape PCA (SFig. 1A) and PCA ratio spectra (SFigs 1B, 1C) including all variables (download SFig. 1 from the publisher). You may get the necessary MRA source script from http://doi.org/10.5281/zenodo.4250142 - tet_001_000000_100x.png, tet_001_000000_150x.png, tet_001_000000_30x.png, etc.: Photographs taken with the Keyence VHX 2000 digital microscope with measurements indicated with yellow lines. Photos were taken either at 30x, 100x, or 150x magnification. Two codes are provides: 1. Numbers at the beginning of each file (tet_001, tet_002, etc.) refer to the code number in the datafile (see above).<br> 2. A code-number composed of a 6 digit number together with the magnification refers to a particular character in the following manner: <em>Code-number Abbrev. Character name</em><br> 000000_30x prn.l Pronotum length<br> 000001_30x prn.b Pronotum breadth<br> 000002_30x prn.h Pronotum height<br> 000003_30x fm3.l Hind femur length<br> 000004_30x fm3.b Hind femur breadth<br> 000005_30x hwi.l Hind wing length<br> 000000_100x fm2.l Mid-femur length<br> 000001_100x fm2.b Mid-femur breadth<br> 000002_100x teg.l Tegmen length<br> 000003_100x teg.b Tegmen breadth<br> 000004_100x hea.b Head breadth<br> 000005_100x vrt.b Vertex breadth<br> 000006_100x fro.h Frons height<br> 000000_150x bt3.l Basitarsus length<br> 000001_150x pu2.l 2nd pulvillus length<br> 000002_150x pu3.l 3rd pulvillus length<br> 000003_150x eye.b Eye breadth<br> 000004_150x eye.h Eye height<br> 000005_150x fl5.l 5th flagellomere length<br> 000006_150x fl5.b 5th flagellomere breadth <strong>Citation of this package</strong><br> Use the citation provided by Zenodo (on this webpage) Contact<br> Hannes Baur
description: {"references": ["Baur, Hannes, & Leuenberger, Christoph. (2020, November 6). Multivariate Ratio Analysis (MRA): R-scripts and tutorials for calculating Shape PCA, Ratio Spectra and LDA Ratio Extractor (Version 1.05). Zenodo. http://doi.org/10.5281/zenodo.4250142", "Moser V, Baur H, Lehmann AW, Lehmann GUC (2021) Two species? - Limits of the species concepts in the pygmy grasshoppers of the Tetrix bipunctata complex (Orthoptera: Tetrigidae). Zookeys 1043: 33-59. https://doi.org/10.3897/zookeys.1043.68316"]}