cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/adf4g-tk389
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-31/rawdata/</p>exsD-mini-cell_target_33.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-31/rawdata/exsD-mini-cell_target_33.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_33_full.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-31/3dimage_105379/exsD-mini-cell_target_33_full.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_33.mrc.mdoc,  64.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-31/rawdata/exsD-mini-cell_target_33.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/chzst-s3f30
title: P aeruginosa del-exsD mutants

description: Pseudomonas aeruginosa, exsD
description: <strong>Tilt Series Date:</strong> 2019-06-29</p>
<strong>Data Taken By:</strong> Steven Wang</p>
<strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -6.0 μm,  magnification: 19500x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Alan Hauser (Northwestern University)</p>
<strong>Purification / Growth Conditions / Treatment:</strong> purified by 3000xg low-speed centrifugation (10 min) to remove filamentous cells. Then do a 15,000xg spin (30 min) to pellet down the mini cells.</p>
<strong>Sample Preparation:</strong> quantifoil grid, 10 nm gold beads, 100% humidty</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-28/rawdata/</p>exsD-mini-cell_target_28.mrc,  Tilt Series (Pixel Size 0.45 nm), 5.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-28/rawdata/exsD-mini-cell_target_28.mrc"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_28_part115_35.rec,  Reconstruction (Pixel Size 0.9 nm), 2.9 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-28/3dimage_105375/exsD-mini-cell_target_28_part115_35.rec"
        > <i class="download icon"></i> Download </a></p>

        exsD-mini-cell_target_28.mrc.mdoc,  60.8 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-06-29-28/rawdata/exsD-mini-cell_target_28.mrc.mdoc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b9as2-dgz15
title: MEF cells - Dynamin2 knockdown

description: Dynamin2 knocked down with retroviral delivery of shRNA to study mitochondrial division. grid info - DG2_grid1.
description: <strong>Tilt Series Date:</strong> 2018-11-27</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> Chun Shik from Chan lab helped design shRNA and provided protocols for viral transduction into Chan lab's MEF cells. Shrawan and Danielle made the cell line. Shrawan FIB-milled and performed ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin (10min)</p>
<strong>Sample Preparation:</strong> Cells were treated with 10 µM FCCP, plunge frozen and FIB-milled.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-18/rawdata/</p>TS11-pt59.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-18/rawdata/TS11-pt59.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS10.mrc,  Tilt Series (Pixel Size 0.432 nm), 1.7 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-18/rawdata/TS10.mrc"
        > <i class="download icon"></i> Download </a></p>

        TS10_4bin_SIRTl20_rotx.rec,  Reconstruction (Pixel Size 0.0 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-27-18/3dimage_102051/TS10_4bin_SIRTl20_rotx.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/x45m1-75y37
title: Ori5 MA

description: Raw data files of Ori5 MA
description: <strong>Tilt Series Date:</strong> 2014-02-12</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Methylomicrobium alcaliphilum</p>
<strong>Strain:</strong> 20Z-ori5</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Marina Kalyuzhnaya @ Univ. Washington provided the culture</p>
<strong>Purification / Growth Conditions / Treatment:</strong> P-medium, 3% NaCl, supplemented with 0.2% methylacetate (3rd transfer from methyalcetate plate)</p>
  scy2014-02-12-3_slicer3919.jpg: surface protein lattice? arrow: vesicle scy2014-02-12-3_slicer3920.jpg: surface proteins; intra-cytoplasmic membranes; granules scy2014-02-12-3_slicer3921.jpg: surface proteins; arrow: cytoskeletal filaments or chemoreceptor arrays? scy2014-02-12-3_slicer3922.jpg: surface proteins; intra-cytoplasmic membrane arrays; vesicle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-3/rawdata/</p>atlas10003.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-3/rawdata/atlas10003.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10003_full.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-12-3/3dimage_38786/atlas10003_full.rec"
        > <i class="download icon"></i> Download </a></p>

        scy2014-02-12-3_slicer16508.png,  Conical spiked structures on outer membrane (pink arrow). .  Semi flat vesicle stacks (green arrow) .  -andrew, 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/scy2014-02-12-3_slicer16508.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.6huxzb
title: Occurrence Download

description: A dataset containing 106636 species occurrences available in GBIF matching the query:
{
 "and" : [
 "GadmGid is one of (PHL.47.6_1, PHL.47_1)",
 "OccurrenceStatus is Present",
 "TaxonKey is Aves",
 "Year 2020-2025"
 ]
}

 The dataset includes 106636 records from 6 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0044999-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/dn73v-xrn07
title: ori5 CH4

description: Raw data files of ori5 CH4
description: <strong>Tilt Series Date:</strong> 2014-02-13</p>
<strong>Data Taken By:</strong> Songye Chen</p>
<strong>Species / Specimen:</strong> Methylomicrobium alcaliphilum</p>
<strong>Strain:</strong> 20Z-ori5</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -12.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Marina Kalyuzhnaya @ Univ. Washington provided the culture</p>
<strong>Purification / Growth Conditions / Treatment:</strong> P-medium, 3% NaCl, supplemented with methane (20% headspace)</p>
  scy2014-02-13-7_slicer3951.jpg: intra-cytoplasmic membrane arrays; surface proteins; granules; broken outer and inner membranes scy2014-02-13-7_slicer3952.jpg: intra-cytoplasmic membrane arrays; surface proteins; granules; flagellum; localized high contrast particles;
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-7/rawdata/</p>atlas40001.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-7/rawdata/atlas40001.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas40001_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 623.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scy2014-02-13-7/3dimage_38881/atlas40001_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        scy2014-02-13-7_slicer16509.png,  1.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/scy2014-02-13-7_slicer16509.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/yv34s-2hw96
title: Myxococcus xanthus PilO-sfGFP

description: Grid box YWC110Strain SA4088
description: <strong>Tilt Series Date:</strong> 2014-07-16</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-56.0°, 56.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor/BatchRunTomo</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 31 hr in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.6</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
  ycw2014-07-16-38_slicer7466.jpg: inner rod of loaded T6SS? ycw2014-07-16-38_slicer7467.jpg: loaded T6SS ycw2014-07-16-38_slicer7468.jpg: loaded T6SS
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-38/rawdata/</p>20140716_YWC110_20010new.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.2 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-38/rawdata/20140716_YWC110_20010new.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140716_YWC110_20010new.rec,  Reconstruction (Pixel Size 1.56 nm), 1.1 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-38/3dimage_44905/20140716_YWC110_20010new.rec"
        > <i class="download icon"></i> Download </a></p>

        20140716_YWC110_20010new_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-16-38/3dimage_44939/20140716_YWC110_20010new_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6md7h-pkm43
title: E.cloacae-typeV-CdiA+

description: Type V secretion system
description: <strong>Tilt Series Date:</strong> 2014-03-03</p>
<strong>Data Taken By:</strong> Poorna Subramanian</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Drs. Davod Low and Chris Hayes, UC Santa Barbara</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning</p>
<strong>Sample Preparation:</strong> Blot time 3s, Blot off-set -4, 3 ul culture</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-25/rawdata/</p>atlas10025.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-25/rawdata/atlas10025.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10025_part121_2.rec,  Reconstruction (Pixel Size 1.92 nm), 801.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2014-03-03-25/3dimage_39561/atlas10025_part121_2.rec"
        > <i class="download icon"></i> Download </a></p>

        psa2014-03-03-25_slicer19360.png,  tubes, 1.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/psa2014-03-03-25_slicer19360.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/p81ag-8me83
title: Mycobacter smegmatis pneumonia

description: KP3 WT (S) M. smegmatis mc2_155
description: <strong>Tilt Series Date:</strong> 2013-09-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Mycobacterium smegmatis</p>
<strong>Strain:</strong> mc2_155</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> UCSF Tomo  gatan K2  Raptor</p>
<strong>Collaborators and Roles:</strong> Koushik Paul / Eric Brown Genentech, Inc. 1 DNA Way South San Francisco, CA 94080 (650) 225-6248 Fax: (650) 225-1107</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Saunton Media (S)</p>
<strong>Sample Preparation:</strong> 3ul + 10nm Au R2/2 quantifoils 4.5 offset 1 blot 2s 1s drainsingle tilt. constant angular increment, step: 1. tilt range: (-60, 60).        dosage: 180/A2. defocus: -8um. magnification: 26000</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-5/rawdata/</p>atlas10005.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-5/rawdata/atlas10005.mrc"
        > <i class="download icon"></i> Download </a></p>

        atlas10005_full.rec,  Reconstruction (Pixel Size 1.56 nm), 445.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2013-09-09-5/3dimage_30577/atlas10005_full.rec"
        > <i class="download icon"></i> Download </a></p>

        amw2013-09-09-5_slicer16498.png,  Another example of a REZ which would be difficult to segment.  Possible flow lines visible. .  -andrew, 409.9 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/Caps/amw2013-09-09-5_slicer16498.png"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v32vz-jz559
title: Myxococcus xanthus delta-tsaP

description: Grid box YWC114
description: <strong>Tilt Series Date:</strong> 2014-08-11</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: -6.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-2/rawdata/</p>20140811_YWC114_10002.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-2/rawdata/20140811_YWC114_10002.mrc"
        > <i class="download icon"></i> Download </a></p>

        20140811_YWC114_10002.rec,  Reconstruction (Pixel Size 1.56 nm), 1.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-2/3dimage_45424/20140811_YWC114_10002.rec"
        > <i class="download icon"></i> Download </a></p>

        20140811_YWC114_10002_full.rec,  Reconstruction (Pixel Size 1.56 nm), 534.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-08-11-2/3dimage_45427/20140811_YWC114_10002_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/sker4-mtc25
title: Myxococcus xanthus PilO-sfGFP

description: Strain SA4088Grid box YWC092
description: <strong>Tilt Series Date:</strong> 2014-04-15</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -8.0 μm,  magnification: 27500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain form Lotte Sogaard-Andersen</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-04-15-3/rawdata/</p>20140415_YWC092_10003.mrc,  Tilt Series (Pixel Size 0.39 nm), 3.7 GB  
        
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        20140415_YWC092_10003_full.rec,  Reconstruction (Pixel Size 1.56 nm), 712.7 MB  
        
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        ycw2014-04-15-3_slicer19079.png,  REZ with possible faint flow lines .  -andrew, 650.1 kB  
        
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DOI: 10.15468/dl.gu8knu
title: Occurrence Download

description: A dataset containing 419 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Eucalyptus sideroxylon",
 "Country is United States of America",
 "HasCoordinate is true"
 ]
}

 The dataset includes 419 records from 29 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0045048-250811113504898/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.