cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14320357
title: Production of Pityrocarpa moniliformis (Benth.) Luckow & R.W. Jobson (Fabaceae) seedlings irrigated with saline water

description: ABSTRACT Saline irrigation water at high levels causes disturbance in the growth of more sensitive plants. The objective of this research was to evaluate the initial growth of Pityrocarpa moniliformis seedlings under different electrical conductivity in irrigation water through physiological and biochemical analyses. The experiment was conducted in a greenhouse with five water electrical conductivities (0.5, 2.0, 4.0, 6.0 and 8.0 dS m-1) and four repetitions of 20 plants each, arranged in a randomized block design. Application of the treatments with irrigation water containing NaCl began at 30 days after sowing. To determine the behaviour of the species, the following variables were analysed: stem diameter, plant height, number of leaves, leaf area, Dickson quality index, shoot dry mass, root dry mass and total dry mass, as well as the biochemical variables, such as the concentrations of total soluble sugars, free proline and chlorophylls a and b. The increase in irrigation water salinity hampered the growth of P. moniliformis seedlings, with electrical conductivity of 0.5 dS m-1 being the limit for maximum production. The results also indicated that the deleterious effects of salt stress on P. moniliformis seedlings variables increase in concentrations of proline, total soluble sugars and betaine glycine.
DOI: 10.6084/m9.figshare.14320360.v1
title: Modeling kinetics of convective drying of Curcuma longa L.

description: ABSTRACT This study aimed to determine drying curves of land saffron (Curcuma longa L.) rhizomes at different temperatures and ventilation conditions to adjust non-linear regression models, and to calculate effective diffusion coefficients and activation energies. Saffron rhizomes were randomly collected in natura with a hoe from the soil in Rio Verde, Goiás, Brazil. They were subsequently sized, sanitized, and sliced into 2.63 ± 0.1 mm thick sections. Rhizomes were dried in an oven with forced air ventilation at 45, 55, 65 and 75 °C for 18, 14, 10 and 9 hours, respectively. As the temperature increased, drying time was reduced. Consequently, moisture content also decreased, facilitating the drying process by decreasing the energy required to remove water molecules. Among the analyzed models, the Midilli model was best adjusted to the data under different drying air conditions. Effective diffusion coefficients (D) were 9.17 × 10-11, 13.33 × 10-11, 20.09 × 10-11, and 35.89 × 10-11 m2 s-1 at 45, 55, 65 and 75 °C, respectively, increasing with higher temperatures. Activation energy for liquid diffusion during drying was 21.186 kJ mol-1.
DOI: 10.6084/m9.figshare.14320360
title: Modeling kinetics of convective drying of Curcuma longa L.

description: ABSTRACT This study aimed to determine drying curves of land saffron (Curcuma longa L.) rhizomes at different temperatures and ventilation conditions to adjust non-linear regression models, and to calculate effective diffusion coefficients and activation energies. Saffron rhizomes were randomly collected in natura with a hoe from the soil in Rio Verde, Goiás, Brazil. They were subsequently sized, sanitized, and sliced into 2.63 ± 0.1 mm thick sections. Rhizomes were dried in an oven with forced air ventilation at 45, 55, 65 and 75 °C for 18, 14, 10 and 9 hours, respectively. As the temperature increased, drying time was reduced. Consequently, moisture content also decreased, facilitating the drying process by decreasing the energy required to remove water molecules. Among the analyzed models, the Midilli model was best adjusted to the data under different drying air conditions. Effective diffusion coefficients (D) were 9.17 × 10-11, 13.33 × 10-11, 20.09 × 10-11, and 35.89 × 10-11 m2 s-1 at 45, 55, 65 and 75 °C, respectively, increasing with higher temperatures. Activation energy for liquid diffusion during drying was 21.186 kJ mol-1.
DOI: 10.6084/m9.figshare.14320362
title: Can inoculation with diazotrophic bacteria decrease the productivity loss of defoliated Vigna unguiculata (L.) Walp?

description: ABSTRACT The objective of this study was to evaluate the effect of artificial defoliation on vegetative and productive stages of cowpea inoculated with diazotrophic bacteria, in two experiments. The first experiment was performed in a greenhouse with 5 × 5 factorial (five defoliation percentages and five N sources), with four repetitions. N sources consisted of bacterial inoculation with strains INPA 03-11B, UFLA 03-84, UFRB FA34C2-2, and two control treatments: i - with N fertilization and ii - blank control, without N fertilization and without inoculation. The second experiment was performed in the field, in a 3 × 5 factorial scheme, with three repetitions. Treatments consisted of three N sources: with N fertilization, with bacterial strain INPA 03-11B that was selected in the first experiment, and five percentages of artificial defoliation. Defoliation percentages for both experiments were 0, 25, 50, 75, and 100%. Artificial defoliation during the vegetative stage caused reduction in the dry mass of bacterial nodules. Inoculation increased plant tolerance to defoliation and enhanced grain nutrient concentration (N and P). Inoculation with the strain INPA 03-11B allowed cowpea plants to tolerate 50% defoliation in the vegetative stage. The mean productivity of cowpea was reduced under &gt; 50% defoliation during the productive stage; therefore, control of defoliating pests until the productive stage is not necessary under field conditions.
DOI: 10.6084/m9.figshare.14320362.v1
title: Can inoculation with diazotrophic bacteria decrease the productivity loss of defoliated Vigna unguiculata (L.) Walp?

description: ABSTRACT The objective of this study was to evaluate the effect of artificial defoliation on vegetative and productive stages of cowpea inoculated with diazotrophic bacteria, in two experiments. The first experiment was performed in a greenhouse with 5 × 5 factorial (five defoliation percentages and five N sources), with four repetitions. N sources consisted of bacterial inoculation with strains INPA 03-11B, UFLA 03-84, UFRB FA34C2-2, and two control treatments: i - with N fertilization and ii - blank control, without N fertilization and without inoculation. The second experiment was performed in the field, in a 3 × 5 factorial scheme, with three repetitions. Treatments consisted of three N sources: with N fertilization, with bacterial strain INPA 03-11B that was selected in the first experiment, and five percentages of artificial defoliation. Defoliation percentages for both experiments were 0, 25, 50, 75, and 100%. Artificial defoliation during the vegetative stage caused reduction in the dry mass of bacterial nodules. Inoculation increased plant tolerance to defoliation and enhanced grain nutrient concentration (N and P). Inoculation with the strain INPA 03-11B allowed cowpea plants to tolerate 50% defoliation in the vegetative stage. The mean productivity of cowpea was reduced under &gt; 50% defoliation during the productive stage; therefore, control of defoliating pests until the productive stage is not necessary under field conditions.
DOI: 10.6084/m9.figshare.14320363
title: Production and quality of cowpea seeds desiccated with saflufenacil and flumioxazin

description: ABSTRACT The use of desiccants allows to anticipate cowpea harvest at a time closer to physiological maturity. The desiccants saflufenacil and flumioxazin promote the drying and falling of leaves and loss of water from seeds. This study aimed to evaluate the effects of saflufenacil and flumioxazin doses applied to the cowpea crop on the production and quality of seeds at harvest and after storage for six months. Two experiments were carried out in a split-plot design (5 × 2). In the first experiment, the main factor consisted of saflufenacil doses (0, 25, 50, 100, and 150 g ai ha-1), while the second factor consisted of evaluation periods (after harvest and six months after harvest). In the second experiment, the main factor consisted of flumioxazin doses (0, 10, 20, 30, and 40 g ai ha-1) and the second factor consisted of the same evaluation periods. The treatments were designed in randomized blocks, with four replications. Saflufenacil application from 25 g ha-1 compromised the yield components and technological quality, while flumioxazin doses did not affect the yield components. Storage at 20 °C for six months decreased the physiological quality of seeds. Flumioxazin application at cowpea harvest promoted uniformity of maturity and harvest anticipation, without compromising the physiological quality after harvest.
DOI: 10.6084/m9.figshare.14320365.v1
title: Quality variables for technological application of cocoa clones from the Brazilian semiarid region

description: ABSTRACT Characterization of cocoa clones produced in the semiarid region is necessary to enlarge the database about these implanted clones and thus enhance the quality of their by-products. Therefore, this study aims to evaluate physical, chemical, and physicochemical characteristics of the CCN 51, CEPEC 2004, CEPEC 2005, and PS 1319 clones, produced in the region of Vale do Jaguaribe in the state of Ceará (Brazil), and to suggest food processes or products for them. The clones were evaluated according to their physical traits (total fruit mass, rind, pulp, seeds, and placenta and pulp with seeds), fruit transverse diameter (FTD), fruit longitudinal diameter (FLD), the ratio FTD/FLD; rind external thickness (ERT), rind internal thickness (IRT), the ratio ERT/IRT, number of seeds, seed thickness, seed transverse diameter (STD), seed longitudinal diameter (SLD), and the ratio STD/SLD, yield, pulp color, chemical traits (humidity, lipids, proteins, ashes, crude fiber, and carbohydrates), and physicochemical traits (titratable acidity, pH, soluble solids, and reducing sugars) were evaluated. The CCN 51 and CEPEC 2005 clones are the most suitable for the process of cocoa fermentation. For desserts, jams, pulp, and nibs for fat-restricted diets, the most suitable clones are CCN 51, CEPEC 2005, PS 1319 and CEPEC 2004, respectively.
DOI: 10.6084/m9.figshare.14320363.v1
title: Production and quality of cowpea seeds desiccated with saflufenacil and flumioxazin

description: ABSTRACT The use of desiccants allows to anticipate cowpea harvest at a time closer to physiological maturity. The desiccants saflufenacil and flumioxazin promote the drying and falling of leaves and loss of water from seeds. This study aimed to evaluate the effects of saflufenacil and flumioxazin doses applied to the cowpea crop on the production and quality of seeds at harvest and after storage for six months. Two experiments were carried out in a split-plot design (5 × 2). In the first experiment, the main factor consisted of saflufenacil doses (0, 25, 50, 100, and 150 g ai ha-1), while the second factor consisted of evaluation periods (after harvest and six months after harvest). In the second experiment, the main factor consisted of flumioxazin doses (0, 10, 20, 30, and 40 g ai ha-1) and the second factor consisted of the same evaluation periods. The treatments were designed in randomized blocks, with four replications. Saflufenacil application from 25 g ha-1 compromised the yield components and technological quality, while flumioxazin doses did not affect the yield components. Storage at 20 °C for six months decreased the physiological quality of seeds. Flumioxazin application at cowpea harvest promoted uniformity of maturity and harvest anticipation, without compromising the physiological quality after harvest.
DOI: 10.6084/m9.figshare.14320365
title: Quality variables for technological application of cocoa clones from the Brazilian semiarid region

description: ABSTRACT Characterization of cocoa clones produced in the semiarid region is necessary to enlarge the database about these implanted clones and thus enhance the quality of their by-products. Therefore, this study aims to evaluate physical, chemical, and physicochemical characteristics of the CCN 51, CEPEC 2004, CEPEC 2005, and PS 1319 clones, produced in the region of Vale do Jaguaribe in the state of Ceará (Brazil), and to suggest food processes or products for them. The clones were evaluated according to their physical traits (total fruit mass, rind, pulp, seeds, and placenta and pulp with seeds), fruit transverse diameter (FTD), fruit longitudinal diameter (FLD), the ratio FTD/FLD; rind external thickness (ERT), rind internal thickness (IRT), the ratio ERT/IRT, number of seeds, seed thickness, seed transverse diameter (STD), seed longitudinal diameter (SLD), and the ratio STD/SLD, yield, pulp color, chemical traits (humidity, lipids, proteins, ashes, crude fiber, and carbohydrates), and physicochemical traits (titratable acidity, pH, soluble solids, and reducing sugars) were evaluated. The CCN 51 and CEPEC 2005 clones are the most suitable for the process of cocoa fermentation. For desserts, jams, pulp, and nibs for fat-restricted diets, the most suitable clones are CCN 51, CEPEC 2005, PS 1319 and CEPEC 2004, respectively.
DOI: 10.6084/m9.figshare.14320366.v1
title: The role of dual oxidases in physiology and cancer

description: Abstract NOX/DUOX enzymes are transmembrane proteins that carry electrons through biological membranes generating reactive oxygen species. The NOX family is composed of seven members, which are NOX1 to NOX5 and DUOX1 and 2. DUOX enzymes were initially called thyroid oxidases, based on their high expression level in the thyroid tissue. However, DUOX expression has been documented in several extrathyroid tissues, mostly at the apical membrane of the salivary glands, the airways, and the intestinal tract, revealing additional cellular functions associated with DUOX-related H2O2 generation. In this review, we will briefly summarize the current knowledge regarding DUOX structure and physiological functions, as well as their possible role in cancer biology.
DOI: 10.6084/m9.figshare.14320366
title: The role of dual oxidases in physiology and cancer

description: Abstract NOX/DUOX enzymes are transmembrane proteins that carry electrons through biological membranes generating reactive oxygen species. The NOX family is composed of seven members, which are NOX1 to NOX5 and DUOX1 and 2. DUOX enzymes were initially called thyroid oxidases, based on their high expression level in the thyroid tissue. However, DUOX expression has been documented in several extrathyroid tissues, mostly at the apical membrane of the salivary glands, the airways, and the intestinal tract, revealing additional cellular functions associated with DUOX-related H2O2 generation. In this review, we will briefly summarize the current knowledge regarding DUOX structure and physiological functions, as well as their possible role in cancer biology.
DOI: 10.6084/m9.figshare.14320368
title: Cockayne Syndrome: The many challenges and approaches to understand a multifaceted disease

description: Abstract The striking and complex phenotype of Cockayne syndrome (CS) patients combines progeria-like features with developmental deficits. Since the establishment of the in vitro culture of skin fibroblasts derived from patients with CS in the 1970s, significant progress has been made in the understanding of the genetic alterations associated with the disease and their impact on molecular, cellular, and organismal functions. In this review, we provide a historic perspective on the research into CS by revisiting seminal papers in this field. We highlighted the great contributions of several researchers in the last decades, ranging from the cloning and characterization of CS genes to the molecular dissection of their roles in DNA repair, transcription, redox processes and metabolism control. We also provide a detailed description of all pathological mutations in genes ERCC6 and ERCC8 reported to date and their impact on CS-related proteins. Finally, we review the contributions (and limitations) of many genetic animal models to the study of CS and how cutting-edge technologies, such as cell reprogramming and state-of-the-art genome editing, are helping us to address unanswered questions.
DOI: 10.6084/m9.figshare.14320368.v1
title: Cockayne Syndrome: The many challenges and approaches to understand a multifaceted disease

description: Abstract The striking and complex phenotype of Cockayne syndrome (CS) patients combines progeria-like features with developmental deficits. Since the establishment of the in vitro culture of skin fibroblasts derived from patients with CS in the 1970s, significant progress has been made in the understanding of the genetic alterations associated with the disease and their impact on molecular, cellular, and organismal functions. In this review, we provide a historic perspective on the research into CS by revisiting seminal papers in this field. We highlighted the great contributions of several researchers in the last decades, ranging from the cloning and characterization of CS genes to the molecular dissection of their roles in DNA repair, transcription, redox processes and metabolism control. We also provide a detailed description of all pathological mutations in genes ERCC6 and ERCC8 reported to date and their impact on CS-related proteins. Finally, we review the contributions (and limitations) of many genetic animal models to the study of CS and how cutting-edge technologies, such as cell reprogramming and state-of-the-art genome editing, are helping us to address unanswered questions.
DOI: 10.6084/m9.figshare.14320371
title: PPRC1, but not PGC-1α, levels directly correlate with expression of mitochondrial proteins in human dermal fibroblasts

description: Abstract The XPC protein, which is mutated in xeroderma pigmentosum (XP) complementation group C (XP-C), is a lesion recognition factor in NER, but it has also been shown to interact with and stimulate DNA glycosylases, to act as transcriptional co-activator and on energy metabolism adaptation. We have previously demonstrated that XP-C cells show increased mitochondrial H2O2 production with a shift between respiratory complexes I and II, leading to sensitivity to mitochondrial stress. Here we report a marked decrease in expression of the transcriptional co-activator PGC-1α, a master regulator of mitochondrial biogenesis, in XP-C cells. A transcriptional role for XPC in PGC-1α expression was discarded, as XPC knockdown did not downregulate PGC-1α expression and XPC-corrected cells still showed lower PGC-1α expression. DNA methylation alone did not explain PGC-1α silencing. In four different XP-C cell lines tested, reduction of PGC-1α expression was detected in three, all of them carrying the c.1643_1644delTG mutation (ΔTG) in XPC. Indeed, all cell lines carrying XPC ΔTG mutation, whether homozygous or heterozygous, presented decreased PGC-1α expression. However, this alteration in gene expression was not exclusive to XPC ΔTG cell lines, for other non-related cell lines also showed altered PGC-1α expression. Moreover, PGC1-α expression did not correlate with expression levels of TFAM and SDHA, known PGC-1α target-genes. In turn, PPRC1, another member of the PGC family of transcription co-activators controlling mitochondrial biogenesis, displayed a good correlation between its expression in 10 cell lines and TFAM and SDHA. Nonetheless, PGC-1α knockdown led to a slight decrease of its target-gene protein level, TFAM, and subsequently of a mtDNA-encoded gene, MT-CO2. These results indicate that PGC-1α and PPRC1 cooperate as regulators of mitochondrial biogenesis and maintenance in fibroblasts.
DOI: 10.6084/m9.figshare.14320371.v1
title: PPRC1, but not PGC-1α, levels directly correlate with expression of mitochondrial proteins in human dermal fibroblasts

description: Abstract The XPC protein, which is mutated in xeroderma pigmentosum (XP) complementation group C (XP-C), is a lesion recognition factor in NER, but it has also been shown to interact with and stimulate DNA glycosylases, to act as transcriptional co-activator and on energy metabolism adaptation. We have previously demonstrated that XP-C cells show increased mitochondrial H2O2 production with a shift between respiratory complexes I and II, leading to sensitivity to mitochondrial stress. Here we report a marked decrease in expression of the transcriptional co-activator PGC-1α, a master regulator of mitochondrial biogenesis, in XP-C cells. A transcriptional role for XPC in PGC-1α expression was discarded, as XPC knockdown did not downregulate PGC-1α expression and XPC-corrected cells still showed lower PGC-1α expression. DNA methylation alone did not explain PGC-1α silencing. In four different XP-C cell lines tested, reduction of PGC-1α expression was detected in three, all of them carrying the c.1643_1644delTG mutation (ΔTG) in XPC. Indeed, all cell lines carrying XPC ΔTG mutation, whether homozygous or heterozygous, presented decreased PGC-1α expression. However, this alteration in gene expression was not exclusive to XPC ΔTG cell lines, for other non-related cell lines also showed altered PGC-1α expression. Moreover, PGC1-α expression did not correlate with expression levels of TFAM and SDHA, known PGC-1α target-genes. In turn, PPRC1, another member of the PGC family of transcription co-activators controlling mitochondrial biogenesis, displayed a good correlation between its expression in 10 cell lines and TFAM and SDHA. Nonetheless, PGC-1α knockdown led to a slight decrease of its target-gene protein level, TFAM, and subsequently of a mtDNA-encoded gene, MT-CO2. These results indicate that PGC-1α and PPRC1 cooperate as regulators of mitochondrial biogenesis and maintenance in fibroblasts.
DOI: 10.6084/m9.figshare.14320375.v1
title: Identification of soybean trans-factors associated with plastid RNA editing sites

description: Abstract RNA editing is a posttranscriptional process that changes nucleotide sequences, among which cytosine-to-uracil by a deamination reaction can revert non-neutral codon mutations. Pentatricopeptide repeat (PPR) proteins comprise a family of RNA-binding proteins, with members acting as editing trans-factors that recognize specific RNA cis-elements and perform the deamination reaction. PPR proteins are classified into P and PLS subfamilies. In this work, we have designed RNA biotinylated probes based in soybean plastid RNA editing sites to perform trans-factor specific protein isolation. Soybean cis-elements from these three different RNA probes show differences in respect to other species. Pulldown samples were submitted to mass spectrometry for protein identification. Among detected proteins, five corresponded to PPR proteins. More than one PPR protein, with distinct functional domains, was pulled down with each one of the RNA probes. Comparison of the soybean PPR proteins to Arabidopsis allowed identification of the closest homologous. Differential gene expression analysis demonstrated that the PPR locus Glyma.02G174500 doubled its expression under salt stress, which correlates with the increase of its potential rps14 editing. The present study represents the first identification of RNA editing trans-factors in soybean. Data also indicated that potential multiple trans-factors should interact with RNA cis-elements to perform the RNA editing.