cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/ywggf-56b37
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-43/rawdata/</p>43.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-43/rawdata/43.mrc"
        > <i class="download icon"></i> Download </a></p>

        43_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-43/3dimage_24430/43_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/127t1-6jz66
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-14/rawdata/</p>14.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-14/rawdata/14.mrc"
        > <i class="download icon"></i> Download </a></p>

        14_part122_24.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-14/3dimage_24388/14_part122_24.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/hya1j-ps984
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-37/rawdata/</p>38.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-37/rawdata/38.mrc"
        > <i class="download icon"></i> Download </a></p>

        38_part122_4.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-37/3dimage_24415/38_part122_4.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/stdc4-v2651
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-17/rawdata/</p>19.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-17/rawdata/19.mrc"
        > <i class="download icon"></i> Download </a></p>

        19_full.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-17/3dimage_24393/19_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/mmwv2-1d941
title: Sulfolobus solfataricus

description: STIV - infected Sulfolobus solfataricus, Sulfolobus turreted icosahedral virus, Taxonomy ID 269145, Sulfolobus solfataricus, thermophile, pyramid, intrapyramidal body, virus infection
description: <strong>Tilt Series Date:</strong> 2010-01-04</p>
<strong>Data Taken By:</strong> Lu Gan</p>
<strong>Species / Specimen:</strong> Sulfolobus solfataricus</p>
<strong>Strain:</strong> 2-1-12</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Å²,  defocus: None μm,  magnification: 18000x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson</p>
<strong>Purification / Growth Conditions / Treatment:</strong> S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.</p>
<strong>Sample Preparation:</strong> Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-40/rawdata/</p>40.mrc,  Tilt Series (Pixel Size 1.26 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-40/rawdata/40.mrc"
        > <i class="download icon"></i> Download </a></p>

        40_part122_5.rec,  Reconstruction (Pixel Size 2.52 nm), 629.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lga2010-01-04-40/3dimage_24418/40_part122_5.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/k8n8t-45v13
title: HeLa (Myo1a KD) - PM damaged

description: HeLa (Kyoto line stably expressing CHMP4B-EGFP at low levels;courtesy MPI) knocked down for Myo1a (double transfection) and laser damaged. Grid - WY46_grid1 and WY46_grid2 (TS1-19 from grid2 and TS20-42 from grid1)
description: <strong>Tilt Series Date:</strong> 2018-06-16</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> HeLa cells</p>
<strong>Strain:</strong> ATCC</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Å²,  defocus: -8.0 μm,  magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD and tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, transfected with siRNA and caused plasma membrane damage using Laser before freezing. Shrawan performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were knocked down for Myo1a with SMART pool siRNA for 48 hr (2 x 24 hr). Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-06-16-40/rawdata/</p>TS37.mrc,  Tilt Series (Pixel Size 0.541 nm), 1.5 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-06-16-40/rawdata/TS37.mrc"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/6etyp-9n120
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-24/3dimage_6813/</p>038.rec,  Reconstruction (Pixel Size 1.922 nm), 91.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-24/3dimage_6813/038.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tv4pq-wrv89
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-28/3dimage_6820/</p>09jul11a_atlasming1_013.rec,  Reconstruction (Pixel Size 1.922 nm), 162.5 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-28/3dimage_6820/09jul11a_atlasming1_013.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/ag6we-qgd39
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-12/3dimage_6801/</p>019.rec,  Reconstruction (Pixel Size 1.922 nm), 123.1 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-12/3dimage_6801/019.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/tp1ew-4bb53
title: Ecoli JM109 Metabolosome

description: Bifurcating tubules/fibres in the periphery of the cells, Pentameric structures, carboxysomes, metabolosomes, pdu enzymes, Periplasmic cone
description: <strong>Tilt Series Date:</strong> 2009-07-09</p>
<strong>Data Taken By:</strong> Alasdair McDowall</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Å²,  defocus: 10.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> Leginon</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD </p>
<strong>Collaborators and Roles:</strong> PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi" </p>
<strong>Purification / Growth Conditions / Treatment:</strong> Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids. </p>
<strong>Sample Preparation:</strong>  Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-11/3dimage_6800/</p>018.rec,  Reconstruction (Pixel Size 1.922 nm), 1.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/amw2009-07-09-11/3dimage_6800/018.rec"
        > <i class="download icon"></i> Download </a></p>