DOI: 10.5061/dryad.r4xgxd2qm
title: Data from: Experimental study on the impact of continuous and dynamic artificial light at night on moths originating from different skyglow conditions

description: Biodiversity is negatively affected by light pollution, caused by
 artificial light at night (ALAN). Light-emitting diodes facilitate new
 lighting technologies to mediate the negative effects of ALAN, such as
 dynamic ALAN where light intensity can be adjusted to traffic density.
 Organisms living in highly light-polluted areas may show adaptations to
 mitigate the negative effects of ALAN. In a split-brood rearing
 experiment, larvae of two moth species (Ochropleura plecta and Agrotis
 exclamationis) originating from low-medium and high-medium skyglow
 populations were grown under either continuous ALAN, dynamic ALAN or
 control-dark conditions. We tested for ALAN effects on larval mortality,
 feeding behaviour, development and body mass, and whether effects depended
 on skyglow levels in the population of origin. Contrary to previous
 studies, we found either no or positive effects of ALAN on larval
 development, with similar or stronger effects of dynamic ALAN compared to
 continuous ALAN. For A. exclamationis, we showed evidence for faster
 development, increased growth rate and higher body mass under ALAN. This
 could reduce larval exposure to parasites and increase fecundity. We found
 no evidence for evolutionary responses in low-medium or high-medium
 skyglow larvae. Our results show that ALAN does not affect larval
 development the same way in all species.
description: # Data from: Experimental study on the impact of continuous and dynamic
 artificial light at night on moths originating from different skyglow
 conditions Dataset DOI: [10.5061/dryad.r4xgxd2qm](10.5061/dryad.r4xgxd2qm)
 ## Description of the data and file structure The dataset "EVdS et
 al-SciRep.xlsx" contains data on larval development. Larvae of two
 moth species (*Agrotis exclamationis* and *Ochropleura
 plecta*) originating from regions with different skyglow levels were grown
 under either: control dark nights, continuous ALAN or dynamic ALAN. During
 their development, we monitored survival, development time, feeding
 behaviour, growth rate and body mass. ### Files and variables #### File:
 EVdS_et_al-SciRep.zip **Description:** This dataset contains all data used
 for our analyses in the R-script ("EVdS et al-SciRep.R"). It
 contains data on larval development. **ID:** individual code **Room:**
 number of the climate chamber **Fam:** individual for the mother
 **Region:** B = province of Brabant, L = province of Luxembourg
 **Skyglow:** skyglow level at population of origin. High = high-medium,
 Low = low-medium **Treatment:** ALAN treatment: Dark = control, Full =
 continuous ALAN, Smart = dynamic ALAN **Site:** sampling site **Sex:** sex
 of the specimen **Spec:** species: AE =* Agrotis exclamationis*, OP =*
 Ochropleura plecta* **Pupa:** day of pupation (days) **Adult:** day of
 adult eclosion (days) **DeadDay:** day of larval death (days) **Dead:**
 died as a larvae (0 = No, 1 = Yes) **Survived:** survived the larval
 stage (0 = No, 1 = Yes) **Moults:** number of registered moults
 **Feed:** feeding frequency (proportion of days eaten) **Eaten:**
 estimated amount of food eaten in the larval stage (food units) **mpupa:**
 pupal mass (g) **madult:** adult mass (g) **loss:** mass loss at eclosion
 (1 - madult/mpupa) **massn:** nth measurement of larval body mass. For AE
 each 5 days, for OP each 7 days (g) **mmax:** maximal larval mass (g)
 **daymax:** day maximal mass was measured (days) **Rexp:** exponential
 growth rate **RexpO:** initial growth rate (first ten days) **RexpE:**
 final growth rate (last ten days) ## Code/software ##### File:
 EVdS_et_al-SciRep.R * Packages: readxl, car, effects, lme4, lmerTest,
 ggplot2, ggthemes, egg, emmeans, survival, survminer, plyr * Functions: *
 sumSE: function to calculate group means and standard errors. These are
 also used to produce the graphs. * Size: funcions to set figure sizes. *
 Statistical tests: * AE vs OP: testing differences in larval development
 between both species * OPlecta: test effects of ALAN treatments, sex and
 skyglow level at population of origin on larval develoment in *O. plecta*
 * Aexclamationis: test effects of ALAN treatments, sex and skyglow level
 at population of origin on larval develoment in *A. exclamationis* * AE
 Exponential growth rate: test effects of ALAN treatments, sex and skyglow
 level at population of origin on growth rate in *A. exclamationis* *
 Figures ...: contains the script to produce the graphs.