DOI: 10.5281/zenodo.4620947
title: Data set associated to simulations performed within the manuscript "Endosperm turgor pressure both promotes and restricts seed growth and size".

description: This dataset contains the following files 2F4-Col0-iku2-Fig.3f and Supp. Fig.9 Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose unesterified pectins are labelled with the 2F4 antibody (channel 2) 2 genotypes: Col0 and iku2 3 independent experiments JIM5-Col0-ap2-Fig. 4d and Supp Fig. 12b Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the JIM5 antibody (channel 2) 2 genotypes: Col0 and ap2-6 2 independent experiments JIM5-Col0-iku2-Fig. 3h and Supp Fig. 10 Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the JIM5 antibody (channel 2) 2 genotypes: Col0 and iku2 3 independent experiments LM19-Col0-ap2-Fig. 4b and Supp Fig. 12a Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the LM19 antibody (channel 2) 2 genotypes: Col0 and ap2-6 2 independent experiments LM19-Col0-iku2-Fig. 3d and Supp Fig. 8 Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the LM19 antibody (channel 2) 2 genotypes: Col0 and iku2 3 independent experiments pELA1-VENUS-Col0-iku2-Fig3c Confocal stack of developing seeds expressing <em>pELA1::3X-VENUS-N7</em> 2 genotypes: Col0 and iku2 1 independent experiments pELA1-VENUS-Col0-iku2-Supp Fig. 6 Confocal stack of developing seeds expressing <em>pELA1::3X-VENUS-N7</em> 2 genotypes: Col0 and iku2 1 independent experiments Seed-size-ap26-iku2-Fig.5e-Supp-Fig. 12c Pictures of dry seeds 4 genotypes: Col0, iku2, ap2-6 and iku2 ap2-6 2 independent experiments Wall-rupture-Col0-iku2-Fig3k Confocal stack of developing seeds expressing <em>LTi6b-GFP </em>(First channel) and dyed with FM4-64 (second channel) imaged after a 40 µm indentation to break testa wall 2 genotypes: Col0 and iku2 2 replicates Wall-rupture-Col0-iku2-Supp Fig. 11 Confocal stack of developing seeds expressing <em>LTi6b-GFP </em>(First channel) and dyed with FM4-64 (second channel) imaged after a 30 µm or a 50µm indentation to break testa wall 2 genotypes: Col0 and iku2 2 replicates 181211-Col0-iku2-Timelapse-Seed-Size Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10 DPA 2 genotypes: Col0 and iku2 190125-Col0-iku2-Timelapse-Seed-Size Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10 DPA (no 9DPA) 2 genotypes: Col0 and iku2 190523-Col0-ede13-Timelapse-Seed-Size Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 9DPA 2 genotypes: Col0 and <em>ede1-3</em> 200724-Col0-iku2-Timelapse-Seed-Size Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10DPA 2 genotypes: Col0 and <em>iku2</em> 200921-Col0-iku2-Timelapse-Seed-Size Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10DPA 2 genotypes: Col0 and <em>iku2</em> 201023-Col0-ede13-Timelapse-Seed-Size Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10DPA 2 genotypes: Col0 and <em>ede1-3</em> 210519-Col0-Timelapse-invitro-Sorbitol Picture of developing WT seeds (Col-0) at 3, 6, 9 and 12DPA Fruits were grown <em>in planta </em>(uncut) or <em>in vitro</em> from 3DPA onwards Fruits growing <em>in vitro</em> were cultivated in ½ MS + 1% Sucrose + 0.1X PPM (Plant Preservative Medium) + 1X Gamborg Vitamins + 0 to 200mM Sorbitol Note that the file containing the pictures from this experiment was too large for Zenodo, the pictures were thus transformed into 8 bit black/white pictures, a 2x2 binning was applied, before a saving into tiff. The original pictures are available at: http://flower.ens-lyon.fr/ 210709-Col0-Timelapse-invitro-Sorbitol Picture of developing WT seeds (Col-0) at 3, 6, 9 and 12DPA Fruits were grown <em>in planta </em>(uncut) or <em>in vitro</em> from 3DPA onwards Fruits growing <em>in vitro</em> were cultivated in ½ MS + 1% Sucrose + 0.1X PPM (Plant Preservative Medium) + 1X Gamborg Vitamins + 0 to 200mM Sorbitol Note that the file containing the pictures from this experiment was too large for Zenodo, the pictures were thus transformed into 8 bit black/white pictures, a 2x2 binning was applied, before a saving into tiff. The original pictures are available at: http://flower.ens-lyon.fr/ data.zip file: Measurements of seed growth tracking experiments performed on WT plants as well as iku2 and ede1-3 mutants. Measurements of endosperm pressure performed on WT plants and iku2 mutants. Simulation results from a parameter space exploration of the system of ODEs we studied within the scope of the work described in the manuscript.