cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.7483265.v1
title: Cervical relaxation for non-surgical uterus access in Santa Inês ewes

description: ABSTRACT The present study was composed by two experiments aiming to develop a cervical dilation technique for non-surgical access to Santa Inês ewe’s uterus. In Experiment 1, thirty ewes underwent four epidural treatments. The three experimental treatments used 2.0mg/kg ketamine. The group receiving this drug alone was denominated KG, whereas other group had ketamine associated with 0.1mg/kg morphine (KM) and KX a third group had ketamine associated with 0.05mg/kg xylazine (KX). Control treatment was 1mL/7.5kg saline solution epidurally (CON). Cervical dilation was evaluated in both experiments by attempting to pass a metal rod through the cervix. In Experiment 2, three different hormonal protocols for cervical dilation were tested in thirty ewes. Epidural anesthesia with 2.0mg/kg ketamine was the control treatment (KG) and was combined with hormonal treatments: Misoprostol (MI); Oxytocin + Estradiol (OE); Misoprostol + Oxytocin + Estradiol (MOE). In Experiment 1 transposition rate was not different among groups. In Experiment 2, OE presented the highest rate (90%) while MOE presented 86.2%, MI 68.9% and CON 62.1%. The study developed a pharmacological protocol that increased cervical transposition making the non-surgical access to the uterus feasible in Santa Inês ewes.
DOI: 10.6084/m9.figshare.7483268.v1
title: University students’ attitudes towards homosexuality: translation and validation of a measurement scale

description: ABSTRACT The official recognition of homosexuality, driven by the legalization of marriage between same-sex couples in Portugal, should have reduced homonegative attitudes and behaviors and, consequently, persecution of non-heteronormative individuals. Based on this assumption, this study was carried out with the objective of evaluating the attitudes of university students (N = 151) regarding homosexuality. To that end, a scale of attitudes towards lesbian and gay men (“The Attitudes Toward Lesbians and Gay Men Scale” - ATLG) has been translated and socioculturally adapted. The results obtained showed low levels of prejudice of university students related to sexual orientation, especially from female respondents. However, although we assume that the respondents' answers may have been conditioned by the social desirability of lowering their levels of prejudice, there were incongruous discourses when dealing with issues such as homoparentality or homophilia, towards which respondents took a more homonegative attitude. The conclusions of this study have showed the need to create conditions for university students, during their education, to reflect on their attitudes towards homosexuality, as well as develop educational processes that include the promotion of respect and healthy coexistence with sexual diversity and the defense of human rights.
DOI: 10.7284/102678
title: pCO2 (Partial Pressure of Carbon Dioxide) data collected during research cruise MGL1109 using a LDEO pCO2 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7483223
title: Sistema Espacial de Suporte à Decisão para Gestão do Combate ao Dengue usando Lógica Fuzzy

description: ABSTRACT A Brasilian public health problem is dengue, whose virus is transmitted by a vector (the mosquito Aedes aegypt). Despite of efforts of health authorities in order to control this disease, the registered cases number has increased over the years. As consequence, we have an epidemiological problem of identifying priority areas to combat it providing support the definition of specific public health policies. This paper aims at the development of a spatial decision support system, which allows to take into account different information aggregated by a fuzzy rule-based system to detect priority areas for intervention on this disease. The system was applied to study of dengue at Paraíba state of Brazil from 2009 to 2013.
DOI: 10.6084/m9.figshare.7483262.v1
title: The Effects of Threonine on Performance Parameters, Carcass Traits, Visceral Organ Indices and Serum Biochemical Parameters of Linwu Ducks, Aged 4 to 8 Weeks

description: ABSTRACT This study was conducted to establish dietary threonine (Thr) levels for Linwu ducks, aged 4 to 8 wk. Experimental diets formulated to contain 0.56, 0.61, 0.66, 0.71, 0.76, and 0.81% Thr fed to Linwu ducks. A total of 360 healthy Linwu female ducks with similar body weight (1183.89±3.83 g) were randomly divided into six groups, with five replicates in each group, and 12 ducks in each replicate. Samples were collected at 8 wk for the determination of growth performance, carcass traits, visceral organ indices, and serum biochemical parameters. As a result of this study, Thr level had no significant influence on the final weight, the daily gain, feed/gain ratio, and average daily intake (p&gt;0.05). Similarly, there were no significant effects of dietary Thr on carcass traits and visceral organ indices (p&gt;0.05). The pancreatic index was highest among all the treatments when the dietary Thr level was 0.66%. The different dietary Thr levels had no significant effect (p&gt;0.05) on the concentration of total protein (TP), triglyceride (TG), total cholesterol (TC), glucose (GLU), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and glutathione (GSH). However, the serum MDA concentration in the 0.66% treatment was lower (P=0.068) than in the other treatments. In conclusion, Thr at 0.66% concentration may have an antioxidant activity and exert positive effect on Linwu ducks.
DOI: 10.6084/m9.figshare.7483214
title: Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom

description: Abstract Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV). Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed proteolytic activity on azocasein with maximum activity at pH 7.0-9.0. It was inhibited by EDTA (a metal chelator that removes zinc, which is essential for enzymatic activity) and no effect was observed with PMSF, iodoacetic acid or pepstatin (inhibitors of serine, cysteine and aspartyl proteases, respectively). Ca2+, Mg2+ and Ba2+ ions increased its activity, while Al3+, Cu2+, Ni2+ and Zn2+ inhibited it. Additionally, ZnCl2 showed a dose dependent inhibition of the enzyme. Lmr-MP activity was also evaluated upon chromogenic substrates for plasma kallikrein (S-2302), plasmin and streptokinase-activated plasminogen (S-2251) and Factor Xa (S-2222) showing the highest activity on S-2302. The activity in different solutions (5 mM or 50 mM ammonium bicarbonate, pH 7.8; 0.1% trifluoroacetic acid + 50% acetonitrile; phosphate buffer saline, pH 7.4; 50 mM sodium acetate, pH 4.0 or ammonium acetate pH 4.5) was also evaluated and the results showed that its activity was abolished at acidic pHs. Its molecular mass (22,858 Da) was determined by MALDI-TOF and about 90% of its primary structure was verified by high-resolution mass spectrometry using HCD and ETD fragmentations and database search against the sequence of closely related species. It is a novel enzyme which shared high identity with other snake venom metalloproteases (svMPs) belonging to the P-I group. Conclusion: The purification procedure achieved a novel pure highly active metalloprotease from LmrV. This new molecule can help to understand the metalloproteases mechanisms of action, the Lachesis envenoming, as well as to open new perspectives for its use as therapeutic tools.
DOI: 10.6084/m9.figshare.7483217
title: Evaluation of family caregiver satisfaction with a mental health inpatient service

description: Abstract Objective To evaluate the satisfaction of family caregivers with a mental health inpatient service in Brazil. Methods This was a cross-sectional study with a quantitative approach. A sample of 80 caretaking family members answered the abbreviated version of the Brazilian Mental Health Services’ Family Satisfaction scale (SATIS-BR) and a sociodemographic questionnaire. Categorical variables were expressed as frequencies and percentages and quantitative variables as means and standard deviations. Interactions among variables and indexes of the scale were analyzed using the Student’s t test, Pearson correlation coefficient and analysis of variance. Results The results showed a high mean overall satisfaction score when considering the categorization of the items of the scale, with higher satisfaction indexes in the ‘Treatment results’ subscale and lower ones in the ‘Reception and competence of staff’ and ‘Privacy and confidentiality’ subscales. In the comparison of the samples studied, greater scores were observed in general satisfaction and in factors in the medical residency care model than in the attending psychiatrist model. There were no significant differences in terms of family member satisfaction in relation to sociodemographic variables. Conclusion Family member satisfaction was high. The need for improvement in aspects related to the infrastructure of services was evident. This paper underlines the importance of continuous and regular evaluations of the services provided, focusing on the satisfaction of users and family members in order to better understand the factors that contribute towards the quality of care provided.
DOI: 10.7284/102682
title: Singlebeam Echosounder data collected during research cruise MGL1109 using a Knudsen 3260 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7483214.v1
title: Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom

description: Abstract Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV). Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed proteolytic activity on azocasein with maximum activity at pH 7.0-9.0. It was inhibited by EDTA (a metal chelator that removes zinc, which is essential for enzymatic activity) and no effect was observed with PMSF, iodoacetic acid or pepstatin (inhibitors of serine, cysteine and aspartyl proteases, respectively). Ca2+, Mg2+ and Ba2+ ions increased its activity, while Al3+, Cu2+, Ni2+ and Zn2+ inhibited it. Additionally, ZnCl2 showed a dose dependent inhibition of the enzyme. Lmr-MP activity was also evaluated upon chromogenic substrates for plasma kallikrein (S-2302), plasmin and streptokinase-activated plasminogen (S-2251) and Factor Xa (S-2222) showing the highest activity on S-2302. The activity in different solutions (5 mM or 50 mM ammonium bicarbonate, pH 7.8; 0.1% trifluoroacetic acid + 50% acetonitrile; phosphate buffer saline, pH 7.4; 50 mM sodium acetate, pH 4.0 or ammonium acetate pH 4.5) was also evaluated and the results showed that its activity was abolished at acidic pHs. Its molecular mass (22,858 Da) was determined by MALDI-TOF and about 90% of its primary structure was verified by high-resolution mass spectrometry using HCD and ETD fragmentations and database search against the sequence of closely related species. It is a novel enzyme which shared high identity with other snake venom metalloproteases (svMPs) belonging to the P-I group. Conclusion: The purification procedure achieved a novel pure highly active metalloprotease from LmrV. This new molecule can help to understand the metalloproteases mechanisms of action, the Lachesis envenoming, as well as to open new perspectives for its use as therapeutic tools.
DOI: 10.6084/m9.figshare.7483217.v1
title: Evaluation of family caregiver satisfaction with a mental health inpatient service

description: Abstract Objective To evaluate the satisfaction of family caregivers with a mental health inpatient service in Brazil. Methods This was a cross-sectional study with a quantitative approach. A sample of 80 caretaking family members answered the abbreviated version of the Brazilian Mental Health Services’ Family Satisfaction scale (SATIS-BR) and a sociodemographic questionnaire. Categorical variables were expressed as frequencies and percentages and quantitative variables as means and standard deviations. Interactions among variables and indexes of the scale were analyzed using the Student’s t test, Pearson correlation coefficient and analysis of variance. Results The results showed a high mean overall satisfaction score when considering the categorization of the items of the scale, with higher satisfaction indexes in the ‘Treatment results’ subscale and lower ones in the ‘Reception and competence of staff’ and ‘Privacy and confidentiality’ subscales. In the comparison of the samples studied, greater scores were observed in general satisfaction and in factors in the medical residency care model than in the attending psychiatrist model. There were no significant differences in terms of family member satisfaction in relation to sociodemographic variables. Conclusion Family member satisfaction was high. The need for improvement in aspects related to the infrastructure of services was evident. This paper underlines the importance of continuous and regular evaluations of the services provided, focusing on the satisfaction of users and family members in order to better understand the factors that contribute towards the quality of care provided.
DOI: 10.6084/m9.figshare.7483223.v1
title: Sistema Espacial de Suporte à Decisão para Gestão do Combate ao Dengue usando Lógica Fuzzy

description: ABSTRACT A Brasilian public health problem is dengue, whose virus is transmitted by a vector (the mosquito Aedes aegypt). Despite of efforts of health authorities in order to control this disease, the registered cases number has increased over the years. As consequence, we have an epidemiological problem of identifying priority areas to combat it providing support the definition of specific public health policies. This paper aims at the development of a spatial decision support system, which allows to take into account different information aggregated by a fuzzy rule-based system to detect priority areas for intervention on this disease. The system was applied to study of dengue at Paraíba state of Brazil from 2009 to 2013.
DOI: 10.6084/m9.figshare.7483205
title: INFRASTRUCTURE AND DEVELOPMENT: what can be expected from the bridge Salvador-Itaparica? Bahia-Brazil

description: This paper aims at analyzing the design of the Road System of the West (SVO), which include the construction of Salvador-Itaparica Bridge, in the light of the discussions on the relationship between infrastructure and economic growth, as well as those that expand the focus to include issues relevant to development. This review was used to assess the project and to examine the social and economic preconditions that are present in four Territories of Identity that will suffer more directly their impacts (Metropolitana de Salvador, Recôncavo, Baixo Sul and Vale do Jiquiriçá). The analysis of the data and information does not allow us to postulate that the project of the Road System of the West and its Bridge could contribute significantly to mitigate the significant inequality and promote the endogenous and sustainable development of the territories.The approach used in this analysis, by rejecting the traditional economic view, extends the discussion of the relationship between investments in infrastructure and regional development using evidences from this case study.
DOI: 10.6084/m9.figshare.7483208
title: The Effect of Different Levels of Lentil by Product on Growth Performance, Carcass Traits and Egg Yield in Quail (Coturnix Coturnix Japonica)

description: ABSTRACT The subject of this study was to evaluate the effects of lentil byproduct (LP) on growth performance, carcass traits and egg yield of quail (Coturnix coturnix Japonica). To achieve this goal, a total of 600 0-day-old quail chicks were used. The birds were divided into 5 groups with 3 replicates. The 1st, 2nd, 3rd, 4th and 5th groups received 0, 5, 10, 15 and 20% lentil byproduct, respectively. All the diets were prepared as isonitrogenous and isocaloric. As a result of this study, the highest live body weights of quails as Laudadio mixed gender were observed in the 3rd (195.5 g) and 5th (195.3 g) groups at the end of the study, however the differences between the control and treatment groups were not significant (p&gt;0.05). Similar results were observed in the carcass traits, as well. The best feed conversion ratio (FCR) was noted in both the 2nd and 3rd groups as 3.04 and it was significantly (p&lt;0.05) different than in the control and other treatment groups. The least feed intake (FI) was observed in the 2nd group. The highest and the lowest egg yield percentages were in the 3rd (90.78 %) and 5th (66.57 %) groups, and differences were significant (p&lt;0.01). Linear increments were observed in the yolk color when LP increased in the diet. As a result, it could be concluded that lentil by product could be added into quail diets up to 15% with no negative effect on live body weight (BW) and carcass traits and to get better yolk color.
DOI: 10.6084/m9.figshare.7483211
title: The Effect of Lentil-By Product on Growth Performance and Carcass Traits of Heavy White Turkeys

description: ABSTRACT The subject of this study was to figure out the effects of lentil by product (LP) on growth performance and carcass traits of the white heavy turkeys. To accomplish this goal, a total of 210 day old big-6 turkey chicks were used. The birds were divided into 7 seven groups with 3 replicates. The 1st, 2nd, 3rd, 4th, 5th, 6th and 7th groups received 0, 5, 10, 15, 20, 25 and 30 % lentil by product, respectively. All the diets were made as izonitrojenic and izocaloric. The highest live body weights of males and females were observed in the control group at the end of the 15th week, which was the time of slaughtering of females, however the differences between the control and the 2nd, 3rd and 4th groups were not significant (p&gt;0.05). The same differences continued for males at the end of the 17th wk of age, which was the time of slaughtering of males. Similar results were observed in carcass traits, as well. The best FCR was noted in the control group and it was significantly (p&lt;0.05) different than in the treatment groups. The lowest and highest feed intake was observed in the 7th and 3rd groups. No significant differences were observed between control and all treatment groups. As a result, it could be said that lentil by product could be added into turkey diets up to 15% with no negative effect on live body weight and carcass traits because there was no significant differences between control and treatment groups (5, 10,15 % LP).
DOI: 10.6084/m9.figshare.7483211.v1
title: The Effect of Lentil-By Product on Growth Performance and Carcass Traits of Heavy White Turkeys

description: ABSTRACT The subject of this study was to figure out the effects of lentil by product (LP) on growth performance and carcass traits of the white heavy turkeys. To accomplish this goal, a total of 210 day old big-6 turkey chicks were used. The birds were divided into 7 seven groups with 3 replicates. The 1st, 2nd, 3rd, 4th, 5th, 6th and 7th groups received 0, 5, 10, 15, 20, 25 and 30 % lentil by product, respectively. All the diets were made as izonitrojenic and izocaloric. The highest live body weights of males and females were observed in the control group at the end of the 15th week, which was the time of slaughtering of females, however the differences between the control and the 2nd, 3rd and 4th groups were not significant (p&gt;0.05). The same differences continued for males at the end of the 17th wk of age, which was the time of slaughtering of males. Similar results were observed in carcass traits, as well. The best FCR was noted in the control group and it was significantly (p&lt;0.05) different than in the treatment groups. The lowest and highest feed intake was observed in the 7th and 3rd groups. No significant differences were observed between control and all treatment groups. As a result, it could be said that lentil by product could be added into turkey diets up to 15% with no negative effect on live body weight and carcass traits because there was no significant differences between control and treatment groups (5, 10,15 % LP).
DOI: 10.7284/102679
title: TSG (Thermosalinograph) data collected during research cruise MGL1109 using a Sea-Bird SBE-21 instrument system onboard the platform RV Marcus G. Langseth

description: Original (unprocessed) data collected during a Field_expedition
DOI: 10.6084/m9.figshare.7483202
title: Identifying microorganisms related to copper bioleaching at 35 and 50°C

description: ABSTRACT Bioleaching is an alternative to pyrometallurgy for the production of metals from low-grade ores containing high level of impurities, once that live pyrometallurgical methods are economically unfeasible. The quantification and identification of those microorganisms related to bioleaching is an important strategy for process control and thus metal recovery. In this regard, molecular biology is one of the main techniques utilized for such objective. This study applied PCR, RFLP and PCR-DGGE techniques to show that the microbial diversity in copper bioleaching columns under investigation is low and the temperature is important to define the species found, with predominance of Acidithiobacillus ferroxidans, at 35°C and Sulfobacillus thermosulfidooxidans at 50°C.
DOI: 10.6084/m9.figshare.7483205.v1
title: INFRASTRUCTURE AND DEVELOPMENT: what can be expected from the bridge Salvador-Itaparica? Bahia-Brazil

description: This paper aims at analyzing the design of the Road System of the West (SVO), which include the construction of Salvador-Itaparica Bridge, in the light of the discussions on the relationship between infrastructure and economic growth, as well as those that expand the focus to include issues relevant to development. This review was used to assess the project and to examine the social and economic preconditions that are present in four Territories of Identity that will suffer more directly their impacts (Metropolitana de Salvador, Recôncavo, Baixo Sul and Vale do Jiquiriçá). The analysis of the data and information does not allow us to postulate that the project of the Road System of the West and its Bridge could contribute significantly to mitigate the significant inequality and promote the endogenous and sustainable development of the territories.The approach used in this analysis, by rejecting the traditional economic view, extends the discussion of the relationship between investments in infrastructure and regional development using evidences from this case study.