cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5281/zenodo.6988420
title: VALERVIDA: Validación de la herramienta digital ALERVIDA

description: Dataset recogido a lo largo de la vida del proyecto VALERVIDA. Se trata de un proyecto financiado por el ISCIII con el que se pretende validar la aplicación móvil denominada ALERVIDA. Esta aplicación tiene como principal objetivo informar al paciente alérgico sobre su estado de salud, recomendarle pautas de vida saludable, prevenir posibles crisis o recaídas, y recomendar al paciente variaciones en la dosis o nuevas vías de medicación así como permitir la comunicación estrecha continúa entre el especialista y el paciente
DOI: 10.6084/m9.figshare.20484482
title: Immunological indices of Giant African Land snails (Archachatina marginata) improved with fixed dose of vitamin C during acute heat stress

description: ABSTRACT. The study determined immunological indices of Giant African Land snail (Archachatina marginata) improved with fixed dose of vitamin C under acute heat stress (AHS). Prior to the AHS, vitamin C was administered for four weeks to two treatment groups, while other two treatment groups were not. Each treatment was monitored, haemolymph collected at 0, 30 and 60 minutes exposure times. Immunological cytokines: interferon gamma (IFN-γ) and interleukin 2 (IL-2); and total haemocyte counts (THC) were determined. Under AHS, vitamin C elevated (p &lt;0.05) IFN-γ production (606.33 ± 302.86) compared to other groups with or without vitamin C administration (7.20 ± 1.58 vs. 73.20 ± 32.23 vs. 7.80 ± 1.36). IL-2 was not affected (p &gt;0.05) by vitamin C under AHS. Highest (p &lt;0.05) THC values was obtained with vitamin C administration under AHS, but reduced under no AHS. Exposure time affected (p &lt;0.05) IFN-γ production and THC values, but not IL-2 (p &gt;0.05). With fixed dose of vitamin C and exposure time, highest (p &lt;0.05) IFN-γ values were obtained under AHS with vitamin C administration at 30 minutes and at 60 minutes in THC, compared to other groups. The study concluded that fixed dose of vitamin C at 150 mg kg-1 of feed was appropriate under AHS to boost the immune system of the animals.
DOI: 10.6084/m9.figshare.20484482.v1
title: Immunological indices of Giant African Land snails (Archachatina marginata) improved with fixed dose of vitamin C during acute heat stress

description: ABSTRACT. The study determined immunological indices of Giant African Land snail (Archachatina marginata) improved with fixed dose of vitamin C under acute heat stress (AHS). Prior to the AHS, vitamin C was administered for four weeks to two treatment groups, while other two treatment groups were not. Each treatment was monitored, haemolymph collected at 0, 30 and 60 minutes exposure times. Immunological cytokines: interferon gamma (IFN-γ) and interleukin 2 (IL-2); and total haemocyte counts (THC) were determined. Under AHS, vitamin C elevated (p &lt;0.05) IFN-γ production (606.33 ± 302.86) compared to other groups with or without vitamin C administration (7.20 ± 1.58 vs. 73.20 ± 32.23 vs. 7.80 ± 1.36). IL-2 was not affected (p &gt;0.05) by vitamin C under AHS. Highest (p &lt;0.05) THC values was obtained with vitamin C administration under AHS, but reduced under no AHS. Exposure time affected (p &lt;0.05) IFN-γ production and THC values, but not IL-2 (p &gt;0.05). With fixed dose of vitamin C and exposure time, highest (p &lt;0.05) IFN-γ values were obtained under AHS with vitamin C administration at 30 minutes and at 60 minutes in THC, compared to other groups. The study concluded that fixed dose of vitamin C at 150 mg kg-1 of feed was appropriate under AHS to boost the immune system of the animals.
DOI: 10.6084/m9.figshare.20484488
title: Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)

description: ABSTRACT. The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.
DOI: 10.6084/m9.figshare.20484488.v1
title: Molecular characterization and association of lactoferrin gene to subclinical mastitis in goats (Capra hircus)

description: ABSTRACT. The study characterized the lactoferrin (Lf) mRNA gene in different goat breeds in the Philippines and determined its association with subclinical mastitis (SCM). The study involved collection of milk at second week of lactation (n=75) and blood samples (n=5) to obtain extracted RNA and using cDNA to amplify Lf gene through polymerase chain reaction. The nucleotide and amino acid sequences were determined and used as reference in the evaluation of phylogenetic relationship. Amplified products were utilized for RFLP analysis before determining the association of the gene with SCM. Results of the study demonstrated that Lf gene in goats registered a molecular weight of 2135. Nucleotide and amino acid sequence of Lf gene revealed high similarity (99%) in Saanen, Anglo-Nubian and Philippine native goats with that of Capra hircus (U53857) Lf gene submitted to GenBank. Phylogenetic studies showed that Lf gene of Anglo-Nubian, Saanen and Native goats clade together with Lf gene of C. hircus (U53857). Three genotypes in goats were documented using the restriction enzymes AluI and HaeIII. Based on the Statistical analysis, association (comp 5.65, p = 0.0308) has been established between the Lf genes of goats with genotype BB to SCM using HaeIII restriction enzyme.
DOI: 10.6084/m9.figshare.20484489
title: Elephant grass silage with addition of regional by-products

description: ABSTRACT. This study examines the effect of adding coffee husks (CH), cacao by-product (CBP) and passion fruit by-product (PBP) (fresh-matter basis) in the silage of elephant grass cv. Napier on nutritional characteristics. The experiment was laid out in a completely randomized design with a 3 × 4 factorial arrangement represented by three additives (CH, CBP or PBP) and four inclusion levels (0, 12, 24 or 36%). Four replicates were used per treatment. The material was ensiled in experimental mini-silos that were opened 60 days later for chemical analysis of the produced silages. The dry matter content of the silages with CH and PBP included at levels greater than 25% was adequate. The silage with PBP inclusion showed the highest crude protein levels and the best results for in vitro dry matter digestibility. In the silage containing CH, the neutral detergent fiber content decreased linearly with increasing inclusion of the husks. The silage with CBP showed the lowest dry matter and crude protein levels and the highest pH. In conclusion, the inclusion of up to 36% PBP in the ensiling of elephant grass is recommended, as the resulting material has potential for use in ruminant feeding at times of forage scarcity.
DOI: 10.6084/m9.figshare.20484489.v1
title: Elephant grass silage with addition of regional by-products

description: ABSTRACT. This study examines the effect of adding coffee husks (CH), cacao by-product (CBP) and passion fruit by-product (PBP) (fresh-matter basis) in the silage of elephant grass cv. Napier on nutritional characteristics. The experiment was laid out in a completely randomized design with a 3 × 4 factorial arrangement represented by three additives (CH, CBP or PBP) and four inclusion levels (0, 12, 24 or 36%). Four replicates were used per treatment. The material was ensiled in experimental mini-silos that were opened 60 days later for chemical analysis of the produced silages. The dry matter content of the silages with CH and PBP included at levels greater than 25% was adequate. The silage with PBP inclusion showed the highest crude protein levels and the best results for in vitro dry matter digestibility. In the silage containing CH, the neutral detergent fiber content decreased linearly with increasing inclusion of the husks. The silage with CBP showed the lowest dry matter and crude protein levels and the highest pH. In conclusion, the inclusion of up to 36% PBP in the ensiling of elephant grass is recommended, as the resulting material has potential for use in ruminant feeding at times of forage scarcity.
DOI: 10.6084/m9.figshare.20484491
title: Leishmania V. braziliensis infection in asymptomatic domestic animals within an endemic region in the Northeast of Brazil

description: ABSTRACT Background: American cutaneous leishmaniasis is a commonly neglected, vector-borne tropical parasitic disease that is a major public health concern in Brazil. Leishmania (Viannia) braziliensis is the main species associated with the disease. Accurate diagnosis is based on epidemiological surveillance, clinical assessment, and laboratory testing. Leishmania (V.) braziliensis has been detected in several wild and synanthropic mammals. Their epidemiological role has not been entirely elucidated. This study aimed to assess potential L. braziliensis infections in asymptomatic domestic animals, by molecular and serological testing in endemic areas, in the metropolitan region of Recife. Methods: Blood samples and conjunctival fluids were collected from 232 animals (canids, felids, equines, and caprines) for the detection of L. braziliensis using molecular tests (conventional and real-time polymerase chain reaction [PCR and qPCR]). For immunological detection, blood samples from 115 dogs were assessed using enzyme-linked immunosorbent assay. Results: Real-time quantitative PCR showed positive results for blood and conjunctival samples in all investigated species. The results of the blood and conjunctival samples were 68.2% and 26.9% in Canis familiaris, 100% and 41.7% in Felis catus, 77.3% and 30.8% in Equus caballus/Equus asinus, and 50% and 33.3% in Capra hircus samples, respectively. Conclusions: Results from this study adds valuable information to our understanding of the role of asymptomatic domestic animals, L. braziliensis life cycle, and American cutaneous leishmaniasis in Northeast Brazil.
DOI: 10.6084/m9.figshare.20484491.v1
title: Leishmania V. braziliensis infection in asymptomatic domestic animals within an endemic region in the Northeast of Brazil

description: ABSTRACT Background: American cutaneous leishmaniasis is a commonly neglected, vector-borne tropical parasitic disease that is a major public health concern in Brazil. Leishmania (Viannia) braziliensis is the main species associated with the disease. Accurate diagnosis is based on epidemiological surveillance, clinical assessment, and laboratory testing. Leishmania (V.) braziliensis has been detected in several wild and synanthropic mammals. Their epidemiological role has not been entirely elucidated. This study aimed to assess potential L. braziliensis infections in asymptomatic domestic animals, by molecular and serological testing in endemic areas, in the metropolitan region of Recife. Methods: Blood samples and conjunctival fluids were collected from 232 animals (canids, felids, equines, and caprines) for the detection of L. braziliensis using molecular tests (conventional and real-time polymerase chain reaction [PCR and qPCR]). For immunological detection, blood samples from 115 dogs were assessed using enzyme-linked immunosorbent assay. Results: Real-time quantitative PCR showed positive results for blood and conjunctival samples in all investigated species. The results of the blood and conjunctival samples were 68.2% and 26.9% in Canis familiaris, 100% and 41.7% in Felis catus, 77.3% and 30.8% in Equus caballus/Equus asinus, and 50% and 33.3% in Capra hircus samples, respectively. Conclusions: Results from this study adds valuable information to our understanding of the role of asymptomatic domestic animals, L. braziliensis life cycle, and American cutaneous leishmaniasis in Northeast Brazil.
DOI: 10.6084/m9.figshare.20484492
title: Medical education in Cardiopulmonary Resuscitation: Didactic transposition from traditional-presential to remote-interactive with simulation

description: Abstract: Introduction: The didactic transposition of the traditional in-person teaching-learning process to the remote-interactive one with simulation in cardiopulmonary resuscitation was a strategy implemented by teachers to promote cognitive, psychomotor, and reflective education on the ethical aspects of first-year medical students in times of pandemic. Experience report: This is an experience report with a descriptive and reflective approach, which is a multiprofessional and multidisciplinary collaborative result of eight teachers, aiming to achieve educational goals. It took place in 2021, at the School of Medicine of Jundiaí (FMJ), in the disciplines of Basic Care and First Aid Notions and Bioethics and Medical Humanities. The activities were planned to be carried out with the 120 enrolled students, through the Google Classroom platform, linked to the institutional account, synchronously and asynchronously. Different teaching strategies, materials, media, and languages were combined with online hypermedia and offline multimedia teaching materials, consisting of different types/formats. Discussion: The transposition was unique and challenging for teachers and students. The collaborative interprofessional teaching work was fundamental for the integration of the two disciplines and the materialization of education in the simulated theoretical and practical dimensions. It is believed that the approach used, combining some technological means, craft simulators, allowed the teaching and learning in basic life support in the cardiopulmonary resuscitation topic in the context of the restrictions imposed by the ongoing pandemic. The students had the opportunity to develop cognitive, technical, and behavioral skills, as well as assess their progress, performing and receiving immediate feedback, as well as through formative assessment without grade assignment. Conclusion: The didactic transposition of the teaching and learning process mediated by technologies allowed students to approach the theoretical content and safely participate in clinical simulations in their homes. However, there is no comparative study that shows that the development was similar to in-person teaching. Consequently, it will be necessary for the pedagogical advisory board to evaluate the possible learning gaps and how they can be overcome throughout the course.
DOI: 10.6084/m9.figshare.20484492.v1
title: Medical education in Cardiopulmonary Resuscitation: Didactic transposition from traditional-presential to remote-interactive with simulation

description: Abstract: Introduction: The didactic transposition of the traditional in-person teaching-learning process to the remote-interactive one with simulation in cardiopulmonary resuscitation was a strategy implemented by teachers to promote cognitive, psychomotor, and reflective education on the ethical aspects of first-year medical students in times of pandemic. Experience report: This is an experience report with a descriptive and reflective approach, which is a multiprofessional and multidisciplinary collaborative result of eight teachers, aiming to achieve educational goals. It took place in 2021, at the School of Medicine of Jundiaí (FMJ), in the disciplines of Basic Care and First Aid Notions and Bioethics and Medical Humanities. The activities were planned to be carried out with the 120 enrolled students, through the Google Classroom platform, linked to the institutional account, synchronously and asynchronously. Different teaching strategies, materials, media, and languages were combined with online hypermedia and offline multimedia teaching materials, consisting of different types/formats. Discussion: The transposition was unique and challenging for teachers and students. The collaborative interprofessional teaching work was fundamental for the integration of the two disciplines and the materialization of education in the simulated theoretical and practical dimensions. It is believed that the approach used, combining some technological means, craft simulators, allowed the teaching and learning in basic life support in the cardiopulmonary resuscitation topic in the context of the restrictions imposed by the ongoing pandemic. The students had the opportunity to develop cognitive, technical, and behavioral skills, as well as assess their progress, performing and receiving immediate feedback, as well as through formative assessment without grade assignment. Conclusion: The didactic transposition of the teaching and learning process mediated by technologies allowed students to approach the theoretical content and safely participate in clinical simulations in their homes. However, there is no comparative study that shows that the development was similar to in-person teaching. Consequently, it will be necessary for the pedagogical advisory board to evaluate the possible learning gaps and how they can be overcome throughout the course.
DOI: 10.6084/m9.figshare.20484495
title: Histomorphometric changes of testicular tissues by season and age of Algerian local donkeys (Equus asinus)

description: ABSTRACT. The present investigation was undertaken to highlight the histomorphometric changes of testicular tissues according to season and age in donkeys (Equus asinus) under northern Algeria conditions. The experiment was conducted from February 2019 to January 2020. A total of 21 sexually mature donkeys were selected randomly. The testis were immediately collected after slaughter for the histological observation. The analysis of the correlation coefficients shows negative correlations between DL and other parameters such as DST, GCEH, TTA and ITSA, ranged between -0.24 and -0.79. Also, there were a high negative correlations between season and the histomorphometric parameters, except DL was revealed positive (r=0.65). On the other hand, there were positive correlations between age and testicular histomorphometrics parameters. The results indicated that DST, GCEH, TTA and ITSA values were significantly higher in winter and autumn seasons than in spring and summer seasons. It is also noteworthy that DST, GCEH, TTA and ITSA values were significantly higher in adult and aged donkeys than in young donkeys. Our comparative analysis of histological parameters, suggests that the sexual activity usually occurs during winter and autumn in local donkeys of Algeria. In addition, our results of histomorphometric of testicular tissues are correlated with age of donkeys.
DOI: 10.6084/m9.figshare.20484495.v1
title: Histomorphometric changes of testicular tissues by season and age of Algerian local donkeys (Equus asinus)

description: ABSTRACT. The present investigation was undertaken to highlight the histomorphometric changes of testicular tissues according to season and age in donkeys (Equus asinus) under northern Algeria conditions. The experiment was conducted from February 2019 to January 2020. A total of 21 sexually mature donkeys were selected randomly. The testis were immediately collected after slaughter for the histological observation. The analysis of the correlation coefficients shows negative correlations between DL and other parameters such as DST, GCEH, TTA and ITSA, ranged between -0.24 and -0.79. Also, there were a high negative correlations between season and the histomorphometric parameters, except DL was revealed positive (r=0.65). On the other hand, there were positive correlations between age and testicular histomorphometrics parameters. The results indicated that DST, GCEH, TTA and ITSA values were significantly higher in winter and autumn seasons than in spring and summer seasons. It is also noteworthy that DST, GCEH, TTA and ITSA values were significantly higher in adult and aged donkeys than in young donkeys. Our comparative analysis of histological parameters, suggests that the sexual activity usually occurs during winter and autumn in local donkeys of Algeria. In addition, our results of histomorphometric of testicular tissues are correlated with age of donkeys.
DOI: 10.6084/m9.figshare.20484497
title: Community health agents with higher education: norms, knowledge and syllabus

description: Abstract This paper aims to analyze how the degrees in Nursing, Social Work, Psychology, or Pedagogy taken by community health agents can influence their knowledge, practices, and the directions of the profession. This is a qualitative, analytical research with triangulation of methods based on the interpretation of the various subjects that dispute the profession. The article is developed in three parts: the first compares normative aspects of the professional categories; the second discusses the knowledge of community health agents after entering higher education and the influence on professional practices; and the third analyzes the syllabuses of Nursing, Social Work, Psychology, Pedagogy, and community health agents as an element of dispute and construction of professional identities. Gaps are pointed out regarding the absence of an ethical-political project and of a teaching and research association proper to community health agents, as well as the necessary dispute of epistemologies and theoretical foundation to achieve a cognitive and professional domain more committed to the transformation of the agents as a subject and of their reality.
DOI: 10.6084/m9.figshare.20484497.v1
title: Community health agents with higher education: norms, knowledge and syllabus

description: Abstract This paper aims to analyze how the degrees in Nursing, Social Work, Psychology, or Pedagogy taken by community health agents can influence their knowledge, practices, and the directions of the profession. This is a qualitative, analytical research with triangulation of methods based on the interpretation of the various subjects that dispute the profession. The article is developed in three parts: the first compares normative aspects of the professional categories; the second discusses the knowledge of community health agents after entering higher education and the influence on professional practices; and the third analyzes the syllabuses of Nursing, Social Work, Psychology, Pedagogy, and community health agents as an element of dispute and construction of professional identities. Gaps are pointed out regarding the absence of an ethical-political project and of a teaching and research association proper to community health agents, as well as the necessary dispute of epistemologies and theoretical foundation to achieve a cognitive and professional domain more committed to the transformation of the agents as a subject and of their reality.