DOI: 10.22002/czpqa-szs88
title: GFP-MreB o/e

description: Raw data files of GFP-MreB o/e
description: <strong>Tilt Series Date:</strong> 2007-04-21</p>
<strong>Data Taken By:</strong> Zhuo Li</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 189.0 eV/Å²,  defocus: -12.0 μm,  magnification: 0x. </p>
<strong>Acquisition Software:</strong> UCSF Tomo</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Strains constructed by Maira Sandkvist (U. Michigan).  Data collected and processed by Zhuo Li.</p>
  zla2007-04-21-2_slicer9365.jpg: Vibrio cholerae .  T6SS? (24 nm) zla2007-04-21-2_slicer9366.jpg: Vibrio cholerae .  filament bundle
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2007-04-21-2/3dimage_801/</p>vcgm422078473cts65w1dot518k12u001bin_full.rec,  Reconstruction (Pixel Size 2.518 nm), 713.0 MB  
        
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        vcgm422078473cts65w1dot518k12u001.mrc,  Tilt Series (Pixel Size 1.259 nm), 738.3 MB  
        
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        dvcgm422078473cts65w1dot518k12u001bin.rec.mrc,  Reconstruction (Pixel Size 2.518 nm) Denoised -lambda 0.5 -iter 15, 1.4 GB  
        
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        zl2007-04-21-2_clip01.rec,  Reconstruction (Pixel Size 2.518 nm) cropped by Sarah, 251.3 MB  
        
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        vcgm8473cts001.dm3,  16.9 MB  
        
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        zl2007-04-21-2ribosegnew.am,  Ribosome segmentation.am file created from post-processed Molmatch data.Created by David J. Rosenman., 8.1 MB  
        
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        zl2007-04-21-2_clip01-label-Filament01.am,  filament segmented by Sarah, 2.0 MB  
        
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        zl2007-04-21-2_clip01-label-Filament02.am,  filament segmented by Sarah, 2.0 MB  
        
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        zl2007-04-21-2_clip01-label-Filament03.am,  filament segmented by Sarah, 2.0 MB  
        
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        zl2007-04-21-2_clip01-label-Filament05.am,  filament segmented by Sarah, 2.0 MB  
        
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        zl2007-04-21-2_clip01-label-Filament06.am,  filament segmented by Sarah, 2.0 MB  
        
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