cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.22002/xnrht-8mr34
title: Agro C58 22C liq

description: Agrobacterium tumefaciens
description: <strong>Tilt Series Date:</strong> 2014-05-27</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> From Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown at 22C and induced with AS for one day in liquid</p>
<strong>Sample Preparation:</strong> Cells were not concentrated prior to freezing</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-10/rawdata/</p>c58_22C_liq10006.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-10/rawdata/c58_22C_liq10006.mrc"
        > <i class="download icon"></i> Download </a></p>

        et2014-05-27-10_full.rec,  Reconstruction (Pixel Size 0.0 nm), 784.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-10/3dimage_49474/et2014-05-27-10_full.rec"
        > <i class="download icon"></i> Download </a></p>

        et2014-05-27-10.tif,  2.2 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-27-10/file_11671/et2014-05-27-10.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n12jy-53x39
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-28/3dimage_68903/</p>12jan06_c105_1_4ADNCos7_AT3BT10011_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-28/3dimage_68903/12jan06_c105_1_4ADNCos7_AT3BT10011_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3BT10011.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-28/rawdata/12jan06_c105_1_4ADNCos7_AT3BT10011.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan06_c105_1_4ADNCos7_AT3BT10011_full.tiff,  file associated to 3D Image #68903, 1.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-28/file_16037/12jan06_c105_1_4ADNCos7_AT3BT10011_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.9r9pxy
title: Occurrence Download

description: A dataset containing 1875 species occurrences available in GBIF matching the query:
{
 "and" : [
 "BasisOfRecord is one of (Human Observation, Observation)",
 "OccurrenceStatus is Present",
 "TaxonKey is Thryophilus nicefori"
 ]
}

 The dataset includes 1875 records from 4 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001841-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/17hn3-gwd19
title: Agro C58 22C liq

description: Agrobacterium tumefaciens
description: <strong>Tilt Series Date:</strong> 2014-05-28</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Å²,  defocus: -10.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> From Pat Zambryski</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown at 22C and induced with AS for one day in liquid</p>
<strong>Sample Preparation:</strong> Cells were not concentrated prior to freezing</p>
  etl2014-05-28-3_slicer4889.jpg: Tube etl2014-05-28-3_slicer4890.jpg: Pilli
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-28-3/rawdata/</p>cd8_22C_liq0004.mrc,  Tilt Series (Pixel Size 0.48 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-28-3/rawdata/cd8_22C_liq0004.mrc"
        > <i class="download icon"></i> Download </a></p>

        cd8_22C_liq0004_full.rec,  Reconstruction (Pixel Size 0.0 nm), 912.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-28-3/3dimage_49490/cd8_22C_liq0004_full.rec"
        > <i class="download icon"></i> Download </a></p>

        slicer000.tif,  3.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-05-28-3/file_11714/slicer000.tif"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/md3q4-ym853
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-1/3dimage_68939/</p>12jan14_C107_1_pNL_DN_AT0man1_ns_full.rec,  Reconstruction (Pixel Size 2.0 nm), 619.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-1/3dimage_68939/12jan14_C107_1_pNL_DN_AT0man1_ns_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT0man1_ns.st,  Tilt Series (Pixel Size 0.5 nm), 916.7 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-1/rawdata/12jan14_C107_1_pNL_DN_AT0man1_ns.st"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT0man1_ns_full.tiff,  file associated to 3D Image #68939, 1.8 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-1/file_16054/12jan14_C107_1_pNL_DN_AT0man1_ns_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/b6fwn-gn441
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-8/rawdata/</p>tomography1arrow.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-8/rawdata/tomography1arrow.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography1arrow.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-8/3dimage_47534/tomography1arrow.rec"
        > <i class="download icon"></i> Download </a></p>

        tomography1arrow_bin_orig_orig_full.rec,  Reconstruction (Pixel Size 0.0 nm), 122.0 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-8/3dimage_48778/tomography1arrow_bin_orig_orig_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/n77j9-80y80
title: Myxococcus xanthus DK1622 wt

description: Imaged by Janelia Titan.
description: <strong>Tilt Series Date:</strong> 2013-07-24</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-24-13/rawdata/</p>20130724_Janelia_Myxo_DK1622_WT0011.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-24-13/rawdata/20130724_Janelia_Myxo_DK1622_WT0011.mrc"
        > <i class="download icon"></i> Download </a></p>

        20130724_Janelia_Myxo_DK1622_WT0011_part131_10.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-24-13/3dimage_28618/20130724_Janelia_Myxo_DK1622_WT0011_part131_10.rec"
        > <i class="download icon"></i> Download </a></p>

        20130724_Janelia_Myxo_DK1622_WT0011_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 258.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-24-13/3dimage_29600/20130724_Janelia_Myxo_DK1622_WT0011_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/9twvf-41x04
title: Myxococcus xanthus JK328

description: Bactofilin BacMNOP knockout strain. Imaged by Janelia Titan
description: <strong>Tilt Series Date:</strong> 2013-07-22</p>
<strong>Data Taken By:</strong> Yi-Wei Chang</p>
<strong>Species / Specimen:</strong> Myxococcus xanthus</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Å²,  defocus: -10.0 μm,  magnification: 26000x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Strain from Martin Thanbichler</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7</p>
<strong>Sample Preparation:</strong> Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-82/rawdata/</p>07222013_Janelia_Myxo_JK328_20024.mrc,  Tilt Series (Pixel Size 0.42 nm), 3.6 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-82/rawdata/07222013_Janelia_Myxo_JK328_20024.mrc"
        > <i class="download icon"></i> Download </a></p>

        07222013_Janelia_Myxo_JK328_20024_part131_8.rec,  Reconstruction (Pixel Size 1.68 nm), 430.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-82/3dimage_28588/07222013_Janelia_Myxo_JK328_20024_part131_8.rec"
        > <i class="download icon"></i> Download </a></p>

        07222013_Janelia_Myxo_JK328_20024_DB.rec,  Reconstruction (Pixel Size 0.0 nm), 258.4 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-07-22-82/3dimage_29615/07222013_Janelia_Myxo_JK328_20024_DB.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/8tcs9-j6r55
title: COS7 VPS4DN+pNLGFP

description: Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug). Sample #C107
description: <strong>Tilt Series Date:</strong> 2012-01-14</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-8/3dimage_68953/</p>12jan14_C107_1_pNL_DN_AT2BT10001_full.rec,  Reconstruction (Pixel Size 2.0 nm), 722.6 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-8/3dimage_68953/12jan14_C107_1_pNL_DN_AT2BT10001_full.rec"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT2BT10001.mrc,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-8/rawdata/12jan14_C107_1_pNL_DN_AT2BT10001.mrc"
        > <i class="download icon"></i> Download </a></p>

        12jan14_C107_1_pNL_DN_AT2BT10001_full.tiff,  file associated to 3D Image #68953, 738.2 kB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-14-8/file_16067/12jan14_C107_1_pNL_DN_AT2BT10001_full.tiff"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.15468/dl.d7z2rh
title: Occurrence Download

description: A dataset containing 1 species occurrences available in GBIF matching the query:
{
 "and" : [
 "TaxonKey is Clematis viticella",
 "Country is Romania",
 "HasCoordinate is true"
 ]
}

 The dataset includes 1 records from 1 constituent datasets; see https://api.gbif.org/v1/occurrence/download/0001842-250827131500795/datasets/export for details.
 
Data from some individual datasets included in this download may be licensed under less restrictive terms.
DOI: 10.22002/tc5ss-ypx41
title: Calreticulin-YFP

description: Correlated light electron microscopy (CLEM) of calreticulin-YFP. The aim of the experiment was to determine whether ribosome associated granules contained calreticulin.
description: <strong>Tilt Series Date:</strong> 2014-10-22</p>
<strong>Data Taken By:</strong> Stephen Carter</p>
<strong>Species / Specimen:</strong> INS-1E Pancreatic Cells expressing Calreticulin-YFP</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Å²,  defocus: -10.0 μm,  magnification: 18000x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Zach Freidberg</p>
<strong>Purification / Growth Conditions / Treatment:</strong> INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.</p>
description: Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-1/rawdata/</p>tomography1middle.mrc,  Tilt Series (Pixel Size 6.0 nm), 3.4 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-1/rawdata/tomography1middle.mrc"
        > <i class="download icon"></i> Download </a></p>

        tomography1middle.rec,  Reconstruction (Pixel Size 24.0 nm), 1.8 GB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-1/3dimage_47353/tomography1middle.rec"
        > <i class="download icon"></i> Download </a></p>

        middle1_full.rec,  Reconstruction (Pixel Size 0.0 nm), 166.3 MB  
        
<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2014-10-22-1/3dimage_47855/middle1_full.rec"
        > <i class="download icon"></i> Download </a></p>
DOI: 10.22002/v9mtw-3ng70
title: COS7 CHMP4A+VPS4DN

description: Cos7 cells transfected with CHMP4A & VPS4DN constructs
description: <strong>Tilt Series Date:</strong> 2012-01-06</p>
<strong>Data Taken By:</strong> Cora Woodward</p>
<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>
<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>
<strong>Tilt Series Settings:</strong>  Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Å²,  defocus: -6.0 μm,  magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSF tomo</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod</p>
<strong>Collaborators and Roles:</strong> Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>
<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>
<strong>Sample Preparation:</strong> manual blotting</p>
description: Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-01-06-1/3dimage_68849/</p>12jan06_c105_1_4ADNCos7_AT1_man2_full.rec,  Reconstruction (Pixel Size 2.0 nm), 499.6 MB  
        
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        12jan06_c105_1_4ADNCos7_AT1_man2.st,  Tilt Series (Pixel Size 0.5 nm), 1.0 GB  
        
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        12jan06_c105_1_4ADNCos7_AT1_man2_full.tiff,  file associated to 3D Image #68849, 1.1 MB  
        
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