DOI: 10.5061/dryad.k0p2ngfm1
title: Programmable liposome organization via DNA origami templates

description: Liposomes are essential vehicles for membrane protein reconstitution and
 drug delivery, making them vital tools in both in vivo and in vitro
 studies. However, the lack of robust techniques for the precise
 arrangement of these synthetic vesicles limits their potential
 applications. Here, we present a modular polymerization platform based on
 square DNA origami to template the formation and organization of
 liposomes. By programming the sequence, number, position, chirality, and
 flexibility of sticky ends on each square, we assemble uniformly sized
 liposomes into diverse two-dimensional (2D) arrays, as well as finite
 lattices and rings. Additionally, we demonstrate stepwise assembly and
 targeted disassembly, enabling dynamic structural control. These complex
 liposome architectures represent a significant advancement in the fields
 of biotechnology, nanotechnology, and bottom-up biology.
description: # Programmable liposome organization via DNA origami templates Dataset
 DOI: [10.5061/dryad.k0p2ngfm1](10.5061/dryad.k0p2ngfm1) ## Description of
 the data and file structure Square DNA origami (SDO) was designed using
 **cadnano**, assembled with an M13-derived scaffold, and purified via
 glycerol gradient centrifugation. **SDO-templated liposomes (STLs)** were
 formed by hybridizing cholesterol-labeled oligos to SDO, followed by lipid
 rehydration, mixing, dialysis, and iodixanol gradient purification.
 Polymerization was controlled by thermal annealing, and dynamic behavior
 was modulated using strand displacement. Samples were analyzed using
 **negative-stain TEM**, **cryo-EM**, and **gel electrophoresis** to assess
 structure, assembly, and polymer formation. Cryo-EM data were acquired on
 a Titan Krios and processed to correct for beam-induced motion. ### Files
 and variables #### File: TEM_images.zip **Description:** This dataset
 contains a curated set of representative negative-stain transmission
 electron microscopy (TEM) images of square DNA origami (SDO) structures
 and SDO-templated liposomes (STLs). Each image provides visual
 documentation of the nanostructures' morphology and assembly quality.
 The image files are named systematically using the following format:
 Structure type: either SDO or STL Description: a brief label describing
 the configuration or experimental condition (e.g., “zigzag array v2”)
 Serial number: a two-digit identifier for indexing purposes (e.g., “03”)
 ## Access information The corresponding figure numbers in the main text
 and Supporting Information are indexed below. \* 1a: SDO_Monomer \* 1b:
 STL_Monomer_fraction5 \* 1c & S4: STL_2D_array; STL_2D_array_TMSD \*
 1d & S6: STL_1D_array; SDO_1D_array \* 1e & S7: STL_3layer_array;
 SDO_3layer_array \* 1f & S8: STL_Zigzag_array_v1; SDO_Zigzag_array_v1
 \* 1g & S9: STL_Zigzag_array_v2; SDO_Zigzag_array_v2 \* 1h & S10:
 STL_4mer_v1; SDO_4mer_v1 \* 1i & S11: STL_8mer_v1; SDO_8mer_v1 \* 2a
 & S14 & S18: STL_5mer_v1; SDO_5mer_v1; STL_9mer_v1; SDO_9mer_v1 \*
 2b & S15 & S19: STL_4mer_v2; SDO_4mer_v2; STL_7mer_v1; SDO_7mer_v1
 \* 2c & S16 & S20: STL_3mer_v1; SDO_3mer_v1; STL_5mer_v2;
 SDO_5mer_v2 \* 2d & S17 & S21: STL_3mer_v2; SDO_3mer_v2;
 STL_5mer_v3; SDO_5mer_v3 \* 2e & S22: STL_4mer_v3; SDO_4mer_v3 \* 2f
 & S23: STL_4mer_v4; SDO_4mer_v4 \* 2g & S24: STL_5mer_v4;
 SDO_5mer_v4 \* 2h & S25: STL_6mer_v1; SDO_6mer_v1 \* 2i & S26:
 STL_6mer_v2; SDO_6mer_v2 \* 2j & S27: STL_8mer_v2 \* 2k & S28:
 STL_9mer_v2; SDO_9mer_v2 \* 2l & S29: STL_7mer_v2; SDO_7mer_v2 \* 2m
 & S30: STL_5mer_v5; SDO_5mer_v5 \* 2n & S31: STL_5mer_v6;
 SDO_5mer_v6 \* 2o & S32: STL_5mer_v7; SDO_5mer_v7 \* 2p & S33:
 STL_5mer_v8; SDO_5mer_v8 \* 2q & S34: STL_7mer_v3 \* 2r & S35:
 STL_6mer_v3; SDO_6mer_v3 \* 2s & S36: STL_6mer_v4; SDO_6mer_v4 \* 2t
 & S34: STL_8mer_v3 \* 2u & S37: STL_7mer_v4; SDO_7mer_v4 \* 2v
 & S38: STL_9mer_v3; SDO_9mer_v3 \* 2w & S39: STL_6mer_v5;
 SDO_6mer_v5 \* 2x & S40-S43: STL_hetero_3mer; STL_hetero_4mer;
 STL_hetero_5mer; STL_hetero_6mer; SDO_hetero_3mer; SDO_hetero_4mer;
 SDO_hetero_5mer; SDO_hetero_6mer \* 3a & S44:
 STL_stepwise_assembly_2mer; STL_stepwise_assembly_3mer;
 STL_stepwise_assembly_4mer \* 3b & S45: STL_dynamic_disassembly;
 SDO_dynamic_disassembly \* S2: SDO_2D_array \* S3: STL_Monomer_fraction3;
 STL_Monomer_fraction4; STL_Monomer_fraction5; STL_Monomer_fraction6 \*
 S12: STL_4mer_v1_RT3h; STL_4mer_v1_4C15h \* S13: SDO_2mer; STL_2mer