cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.5061/dryad.wwpzgmsjh
title: Incucyte data for progeny virus derived from insect cells with or without methyltransferase inhibitor

description: Arthropod endosymbiont Wolbachia pipientis is part of a global biocontrol
 strategy aimed at reducing the spread of mosquito-borne RNA viruses such
 as alphaviruses. Our prior work examining Wolbachia-mediated pathogen
 blocking has demonstrated (i) the importance of a host cytosine
 methyltransferase, DNMT2, in Drosophila, and (ii) viral RNA as a target
 through which pathogen-blocking is mediated. Here we report on the role of
 DNMT2 in Wolbachia induced virus inhibition of alphaviruses in Aedes sp..
 Mosquito DNMT2 levels were altered in the presence of both viruses and
 Wolbachia, albeit in opposite directions. Elevated levels of DNMT2 in
 mosquito salivary glands induced by virus infection were suppressed in
 Wolbachia colonized animals coincident with a reduction of virus
 replication, and decreased infectivity of progeny virus. Ectopic
 expression of DNMT2 in cultured Aedes cells was proviral increasing
 progeny virus infectivity, and this effect of DNMT2 on virus replication
 and infectivity was dependent on its methyltransferase activity. Finally,
 examination of the effects of Wolbachia on modifications of viral RNA by
 LC-MS showed a decrease in the amount of 5-methylcytosine modification
 consistent with the down-regulation of DNMT2 in Wolbachia colonized
 mosquito cells and animals. Collectively, our findings support the
 conclusion that disruption of 5-methylcytosine modification of viral RNA
 is an important mechanism operative in pathogen blocking. These data also
 emphasize the essential role of epitranscriptomic modifications in
 regulating fundamental processes of virus replication and transmission.
description: We used a
 fluorescently-tagged CHIKV reporter virus (CHIKV-mKate) to examine the
 effect of DNA MTase inhibitor DAC5 on virus replication in
 <em>Wolbachia-</em>free and
 <em>Wolbachia-</em>colonized <em>Aedes albopictus
 </em>cells using the Incucyte live-cell imaging
 platform. Fluorescent protein expression was used as a proxy of virus
 spread in cells pretreated with and without the inhibitor (DMSO),
 following synchronized virus adsorption at 4ºC. Post adsorption, cell
 monolayers were extensively washed with 1XPBS to remove any unbound
 viruses, followed by the addition of warm media (37ºC) to initialize virus
 internalization and infection. Virus spread was measured over 50 hours by
 quantifying mean virus-encoded red fluorescent reporter (mKate) expression
 observed over four distinct fields of view taken per well every
 2-hours.
DOI: 10.48689/wy7f-4m43
title: Stormwater Mains (West Vancouver)
DOI: 10.48689/k3nk-rn18
title: Stormwater Mains and Culverts (Burnaby)
DOI: 10.5061/dryad.dr7sqv9xp
title: Environmental heterogeneity shapes physiological traits in tropical direct-developing frogs

description: 1. Tropical ectotherm species tend to have narrower physiological limits
 than species from temperate areas. As a consequence, tropical species are
 considered highly vulnerable to climate change since minor temperature
 increases can push them beyond their physiological thermal tolerance.
 Differences in physiological tolerances can also be seen at finer
 evolutionary scales, such as among populations of ectotherm species along
 elevation gradients, highlighting the physiological sensitivity of such
 organisms. 2. Here we analyze the influence of elevation and bioclimatic
 domains, defined by temperature and precipitation, on thermal
 sensitivities of a terrestrial direct-developing frog (Craugastor
 loki) in a tropical gradient. We address the following
 questions: i) Does preferred temperature vary with elevation and
 among bioclimatic domains? ii) Do thermal tolerance
 limits, i.e. critical thermal maximum and critical thermal minimum vary
 with elevation and bioclimatic domains?, and iii) Are populations
 from high elevations more vulnerable to climate warming?  3. We
 found that along an elevation gradient body temperature decreases as
 environmental temperature increases. The preferred temperature tends to
 moderately increase with elevation within the sampled bioclimatic domains.
 Our results indicate that the ideal thermal landscape for this species is
 located at mid-elevations, where the thermal accuracy (db) and thermal
 quality of the environment (de) are suitable. The critical thermal maximum
 is variable across elevations and among the bioclimatic domains,
 decreasing as elevation increases. Conversely, the critical thermal
 minimum is not as variable as the critical thermal maximum.  4.
 Populations from the lowlands may be more vulnerable to future increases
 in temperature. We highlight that the critical thermal maximum is related
 to high temperatures exhibited across the elevation gradient and within in
 each bioclimatic domain, therefore, it is a response to high environmental
 temperatures.
description:  
description: Databases contain the following information: 
 The file
 named <strong>Crau_dfnight1 </strong>contains the
 <b>Body temperature, Air and substrate
 temperature </b>data. The file
 named <strong>Crau_tsel
 </strong>contains the<strong> Preferred temperature
 (Tsel) </strong>data. The file
 named <strong>Thermal_limits_WT_CT </strong>contains the
 following info: Critical thermal maximum
 (<strong>CTmax</strong>) Critical thermal
 minimum (<strong>CTmin</strong>) Thermal
 tolerance or Thermal breath (<strong>TTR</strong>)
 Warming tolerance (<strong>WT</strong>)
 Cooling tolerance Maximum daily temperature
 (<strong>Tmax_hab</strong>) Minimum daily
 temperature (<strong>Tmin-hab)</strong>
DOI: 10.48689/y3jd-tc50
title: Stormwater Mains and Culverts (White Rock)
DOI: 10.7280/d1q699
title: Heterogeneity and chemical reactivity of the remote Troposphere defined by aircraft measurements

description: The NASA Atmospheric Tomography (ATom) mission built a photochemical
 climatology of air parcels based on in situ measurements with the NASA
 DC-8 aircraft along objectively planned profiling transects through the
 middle of the Pacific and Atlantic Oceans.  ATom measured
 numerous gases and aerosols, particularly the gaseous species driving the
 chemical budgets of O3 and CH4:  i.e., O3, CH4, CO, C2H6, higher
 alkanes, alkenes, aromatics, NOx, HNO3, HNO4, peroxyacetylnitrate, other
 organic nitrates, H2O, HCHO, H2O2, and CH3OOH.  From the 10 s (2
 km) merged observations, a modeling data stream (MDS) based on
 observations of the core species, consisting of 146,494 distinct air
 parcels has been constructed from the 4 ATom deployments, providing a
 continuous data stream for initializing global chemistry models and
 calculating the 24-hour chemical tendencies.  Tendencies derived
 from 6 chemistry models using the ATom-1 MDS tend to agree and show a
 highly heterogeneous troposphere where globally 10% of the parcels control
 as much as 40% of the budget of O3 and CH4.  Surprisingly,
 modeled probability distributions (100-km cells) match ATom statistics (2
 km parcels), indicating that the majority of the observed heterogeneity
 can be resolved with current global chemistry models.  On the
 other hand, the models' own chemical climatologies underestimate O3
 production below 4 km in both Pacific and Atlantic basins because they
 have lower NOX levels than observed.
DOI: 10.48689/qwqy-tq48
title: Stormwater Outfalls (Coquitlam)
DOI: 10.48689/6j6z-7y73
title: Stormwater Outlets (Abbotsford)
DOI: 10.5061/dryad.08kprr528
title: Data from: The DNA history of a lonely oak: Quercus humboldtii phylogeography in the Colombian Andes

description: The climatic and geological changes that occurred during the Quaternary,
 particularly the fluctuations during the glacial and interglacial periods
 of the Pleistocene, shaped the population demography and geographic
 distribution of many species. These processes have been studied in several
 groups of organisms in the northern hemisphere, but their influence on the
 evolution of Neotropical montane species and ecosystems remains unclear.
 This study contributes to the understanding of the effect of climatic
 fluctuations during the late Pleistocene on the evolution of Andean
 mountain forests. Firstly, by describing the nuclear and plastidic DNA
 patterns of genetic diversity, structure, historical demography and
 landscape connectivity of Quercus humboldtii, which is a typical species
 in northern Andean montane forests. Then, by comparing these patterns with
 the palynological and evolutionary hypotheses postulated for montane
 forests of the Colombian Andes under climatic fluctuation scenarios during
 the Quaternary. Our results indicated that populations of Q. humboldtii
 have high genetic diversity, a lack of genetic structure, and a historical
 increase in connectivity from the Last Glacial Maximum (LGM) to the
 present. Furthermore, our results showed a dramatic reduction in the
 effective population size followed by an expansion before the LGM, which
 coincides with the results found by palynological studies, suggesting a
 change in dominance in Andean forests that may be related to ecological
 factors rather than climate change.
description: Methodology for generation of the data files can be found on
 Zorrilla-Azcué et al., 2021
description: <strong>SSR_and_ENM_data_Qhumboldtii.xlsx</strong> -
 Contains 3 sheets:           
  <strong>nSSR</strong> -  Contains genotypes for 10
 chloroplast microsatellite (nSSR) loci for each <em>Quercus
 humboldtii</em> sample. Individual and population codes are also
 given (Sample ID and Pop ID). First row contains column headers and
 microsatellite code names (full names can be found in Zorrilla-Azcué et
 al., 2021).             <strong>cpSSR
 </strong>- Contains data for 9 chloroplast microsatellite (cpSSR)
 loci for each <em>Quercus humboldtii</em> sample. Individual
 and population codes are also given (Sample ID and Pop ID). First row
 contains column headers and microsatellite code names (full names can be
 found in Zorrilla-Azcué et al., 2021).         
 <strong>ENM_occurrence_records </strong>- Contains final
 occurrence points used to construct ecological niche models for
 <em>Quercus humboldtii</em>. This dataset is derived from the
 downloaded data in GBIF on 03 July 2019
 https://doi.org/10.15468/dl.cnhmvu
DOI: 10.48689/k335-sc81
title: Strait of Georgia Ecosystem Modelling (STRATOGEM)
DOI: 10.5061/dryad.rr4xgxd7t
title: Beyond Hermetia illucens: The garden soldier fly Exaireta spinigera as a potential bioconverter of food waste

description: Using insects to convert food waste into useable products, such as
 livestock feed, is an elegant solution to the twin crises of waste
 disposal and food production.  To date, the black soldier fly
 (Hermetia illucens) has garnered intense research interest as an excellent
 bioconverter that consumes wastes and converts them into high protein
 larval biomass that can be fed to livestock or converted into
 biodiesel.  However, other members of the soldier fly family
 (Stratiomyidae) have received far less attention despite similar life
 history traits.  Here we examine the seasonality and performance
 (protein content and protein conversion efficiency) of larval Australian
 garden soldier flies, Exaireta spinigera.  We first examined the
 state of knowledge about E. spinigera’s biology and life history via a
 literature search. Next, we used a global biodiversity database to infer
 the seasonality of E. spinigera in Australia. Finally, we conducted
 outdoor feeding experiments to investigate protein content and protein
 conversion efficiency of larvae fed wastes ranging from 100% fruit and
 vegetable waste (low protein) to 100% poultry feed (higher protein).
 Exaireta spinigera appears to be a winter active species, with reported
 sightings peaking during cooler months of the year. Exaireta spinigera
 larvae also dominated outdoor bucket traps during the winter, when H.
 illucens was otherwise inactive. Exaireta spinigera larvae had decreased
 protein content and decreased protein conversion efficiency when fed foods
 that contained a high proportion of low-protein food waste. However even
 in the 100% food waste treatment, the protein content of E. spinigera was
 relatively high at 38.6% crude protein. We suggest that E. spinigera is an
 excellent candidate for further research as it can convert low quality
 food waste into high protein larvae at colder temperatures than H.
 illucens. Our results highlight the benefits of investigating a wider
 range of dipteran species for their bioconversion abilities.
description: We investigated the performance ( protein content and protein
 conversion efficiency) of the native Australian garden soldier fly,
 <i>Exaireta spinigera</i>.  Wild fly larvae were fed a
 low-protein mixture of household fruit and vegetable waste, supplemented
 with varying amounts of a higher protein poultry feed. Treatments ranged
 from a 100% food waste (lowest protein) to a 100% poultry feed (highest
 protein) treatment. The experiment was conducted in the field in
 April-August (Australian autumn/winter).
description: See metadata.csv for full description of terms
 FW = food waste GSF = garden soldier
 fly BSFL = Black soldier fly
DOI: 10.48689/g1nj-tq97
title: Strait of Georgia Synoptic Bottom Trawl Survey
DOI: 10.48689/3t4f-dv82
title: Strait of Georgia marine near-surface weather at an hourly interval, 2010-2018
DOI: 10.5061/dryad.18931zcx1
title: Environmental DNA metabarcoding reveals and unpacks a biodiversity conservation paradox in Mediterranean marine reserves

description: Although we are currently experiencing worldwide biodiversity loss, local
 species richness does not always decline under anthropogenic pressure.
 This conservation paradox may also apply in protected areas but has not
 yet received conclusive evidence in marine ecosystems. Here, we survey
 fish assemblages in six Mediterranean no-take reserves and their adjacent
 fishing grounds using environmental DNA (eDNA) while controlling for
 environmental conditions. We detect less fish species in marine reserves
 than in nearby fished areas. The paradoxical gradient in species richness
 is accompanied by a marked change in fish species composition under
 different managements. This dissimilarity is mainly driven by species that
 are often overlooked by classical visual surveys but detected with eDNA:
 cryptobenthic, pelagic and rare fishes. These results do not negate the
 importance of reserves in protecting biodiversity but shed new light on
 how under-represented species groups can positively react to fishing
 pressure and how conservation efforts can shape regional biodiversity
 patterns.
description: 30 L of seawater were collected along 2 km transects and filtered
 using 0.20-µM filtration capsules. Environmental DNA was extracted and
 amplified by PCR with the fish-specific primer pair teleo targeting a 70
 bp fragment at the end of the mitochondrial DNA 12S rRNA gene. PCR
 reactions were carried out in 12 replicates per sample and unique tags
 were given to each sample. A paired-end sequencing (2x125 bp) was carried
 out on a MiSeq (Illumina, San Diego, CA, USA).
description: Runs_a Compressed file containing the
 compressed forward and reverse sequence files of runs 1 through
 4. corr_tag File linking the
 sequence files to the sample information.<br> column 1: run
 code<br> column 2: run type<br> column 3: project
 name<br> column 4: sample name (lab identifier, starts with
 "SPY")<br> column 5: sample name with PCR replicate
 number<br> column 6: tag name = position on plate<br> column
 7: tag sequence<br> column 8: forward primer sequence<br>
 column 9: reverse primer sequence
 corr_field <br> File linking the
 sample laboratory ID to the sample (field) metadata. <br> Assigning
 the field ID allows to run the analyses on
 https://github.com/eboulanger/MEDeDNA--reserves<br> column 1: sample
 lab code<br> column 2: sample field code<br> column 3:
 sampling date<br> column 4: longitude coordinate of the transect
 center<br> column 5: latitude coordinate of the transect
 center MEDeDNA_refdb_teleo New
 mediterranean fish reference sequences for the teleo barcode.<br>
 column 1: local reference database identifier<br> column 2: lab
 code<br> column 3: field code<br> column 4: species scientific
 name<br> column 5: teleo sequence<br>
DOI: 10.48689/tend-p216
title: Strait of Georgia, Creel Summary
DOI: 10.48689/4nwe-np10
title:  Strategies for Restoring and Developing Fish Habitats in the Strait of Georgia - Puget Sound Inland Sea, Northeast Pacific Ocean
DOI: 10.5061/dryad.bcc2fqzbx
title: Knowledge and awareness of Covid-19 in eye care

description: Background: Nepal is currently under a lockdown due to the COVID-19
 pandemic. There are concerns regarding misinformation circulating on
 social media. This study aimed to analyse the knowledge and awareness of
 COVID-19 amongst eye care professionals in Nepal. Methodology: We invited
 600 participants from 12 ophthalmic centres across Nepal to complete a
 qualitative, anonymous online survey. Altogether, 25 questions (both open
 and closed-ended) were used. An overall performance score was calculated
 from the average of the 12 Knowledge questions for all the participants.
 Results: Of the 600 eye care professionals invited, 310 (51%) participated
 in the survey. The symptoms of COVID-19 were known to 94%, whilst only 49%
 of the participants were aware how the disease was transmitted, with 54%
 aware that anyone can be infected with SARS-CoV-2. Almost 98% of
 participants recognized the World Health Organization’s (WHO) awareness
 message, but surprisingly, 41% of participants felt that consumption of
 hot drinks helps to destroy the virus, in contradiction to WHO
 information. Importantly, 95% of the participants were aware of personal
 protective equipment (PPE) and what the acronym stands for. Social
 distancing was felt to be key to limiting the disease spread; whilst 41%
 disagreed that PPE should be mandatory for eye care practitioners.
 Conclusion: There is still considerable scope to improve the knowledge of
 COVID-19 amongst ophthalmic professionals in Nepal. Opinion is also split
 on measures to prevent transmission, with misinformation potentially
 fuelling confusion. It is recommended to follow WHO and national
 guidelines, whilst seeking published scientific evidence behind any
 unofficial statements, to accurately inform one’s clinical practice.
DOI: 10.48689/mcz2-sk59
title: Stratogem (2002-2003)
DOI: 10.48689/0kht-8x69
title: Stream Inventory Sample Sites
DOI: 10.5061/dryad.p2ngf1vq8
title: Fire alters diversity, composition and structure of dry tropical forests in the Eastern Ghats

description: Fire is known to have dramatic consequences on forest ecosystems around
 the world, and on the livelihoods of forest-dependent people. While the
 Eastern Ghats of India have high abundances of fire-prone dry tropical
 forests, little is known about how fire influences the diversity,
 composition and structure of these communities. Our study aims to fill
 this knowledge gap by examining the effects of presence and absence of
 recent fire on tropical dry forest communities within Kadiri watershed,
 Eastern Ghats. We sampled plots with and without evidence of recent fire
 in the Eswaramala Reserve Forest in 2008 and 2018. Our results indicate
 that even though stem density increases in the recently burned areas,
 species richness is lower because communities become dominated by a few
 species with fire resistance and tolerance traits, such as thick bark and
 clonal sprouting. Further, in the presence of fire, the size structure of
 these fire-tolerant species shifts towards smaller-sized, resprouting
 individuals. Our results demonstrate that conservation actions are needed
 to prevent further degradation of forests in this region and the ecosystem
 services they provide.
description: At each sampling location, we sampled circular plots of 10m
 radius. At each plot, all individuals of tree species of basal girth
 &gt;2cm were identified and counted in both 2008 and 2018. In 2018, we
 measured size of every stem. In 2008, there were 9 sampling locations with
 2-6 plots in each location, each plot separated by a minimum of 100 m. In
 2018, there were 10 locations, each with 2-8 plots separated by a minimum
 of 100 m. We include plots that are atleast 500m away from the nearest
 human habitation and have atleast two individual tree stems. We document
 evidence of recent fires by visual estimation of plots.
description: The dataset contains
 plotwise tree species abundances for both sampling years 2008 and 2018 and
 size of every stem for 2018.
DOI: 10.48689/5t6x-jz61
title: Stream Networks (West Vancouver)
DOI: 10.5061/dryad.000000039
title: Data from: Indirect genetic and environmental effects on behaviours, morphology, and life-history traits in a wild Eastern chipmunk population

description: Additive genetic variance in a trait reflects its potential to respond to
 selection, which is key for adaptive evolution in the wild. Social
 interactions contribute to this genetic variation through indirect genetic
 effects —the effect of an individual’s genotype on the expression of a
 trait in a conspecific. However, our understanding of the evolutionary
 importance of indirect genetic effects in the wild and of their strength
 relative to direct genetic effects is limited. In this study, we assessed
 how indirect genetic effects contribute to genetic variation of
 behavioural, morphological, and life history traits in a wild Eastern
 chipmunk population. We also compared the contribution of direct and
 indirect genetic effects to traits evolvabilities and related these
 effects to selection strength across traits. We implemented a novel
 approach integrating the spatial structure of social interactions in
 quantitative genetic analyses, and supported the reliability of our
 results with power analyses. We found indirect genetic effects for
 trappability and relative fecundity, little direct genetic effects in all
 traits and a large role for direct and indirect permanent environmental
 effects. Our study highlights the potential evolutionary role of social
 permanent environmental effects in shaping phenotypes of conspecifics
 through adaptive phenotypic plasticity.
description: See methods section in the paper
description: See README file
DOI: 10.48689/3rhg-t073
title: Streams (Sunshine Coast Regional District)
DOI: 10.48689/n561-f505
title: Streamside Protection and Enhancement Areas (Coquitlam)
DOI: 10.5061/dryad.9cnp5hqhj
title: Audio and 3D flight-track recordings of mosquito responses to opposite-sex sound-stimuli

description: This dataset is the collection of audio and 3D-tracked flight responses of
 free-flying, swarming, Anopheles coluzzii, male and female mosquitoes to
 the played-back sound of opposite-sex, free-flying, swarming, Anopheles
 coluzzii and Anopheles gambiae s.s. mosquitoes. 
description: 3D-tracking of individual swarming mosquitoes (from the Trackit
 software) and sound recordings were used to measure the mosquito response
 to sound stimuli. The 3D-flight trajectories (positions
 and linear velocities) along time and for each swarming mosquito are given
 in a CSV file. The sounds are 8 kHz/24bit WAV files and
 their durations are ~1h per replicate. There are 10
 replicates for the males and 13 replicates for the females. 
description: See readme.txt file for folder and file description.
DOI: 10.48689/gbb9-0t24
title: Studies of intertidal vascular plants,especially sedge (Carex lyngbyei),on the disrupted Squamish River delta, British Columbia
DOI: 10.48689/t2sd-f641
title:  Sturgeon Sites for the Lower Mainland Region (2013)