DOI: 10.5061/dryad.369q9
title: Data from: Effects of gene flow on phenotype matching between two varieties of Joshua tree (Yucca brevifolia; Agavaceae) and their pollinators

description: In animal-pollinated plants, local adaptation to pollinator behaviour or
 morphology can restrict gene flow among plant populations; but gene flow
 may also prevent divergent adaptation. Here, we examine possible effects
 of gene flow on plant-pollinator trait matching in two varieties of Joshua
 tree (Agavaceae: Yucca brevifolia). The two varieties differ in strikingly
 in floral morphology, which matches differences in the morphology of their
 pollinators. However, this co-divergence is not present at a smaller
 scale: within the two varieties of Joshua tree, variation in floral
 morphology between demes is not correlated with differences in moth
 morphology. We use population genetic data for Joshua tree and its
 pollinators to test the hypotheses that gene flow between Joshua tree
 populations is structured by pollinator specificity, and that gene flow
 within the divergent plant-pollinator associations “swamps” fine-scale
 co-adaptation. Our data show that Joshua tree populations are structured
 by pollinator association, but the two tree varieties are only weakly
 isolated—meaning that their phenotypic differences are maintained in the
 face of significant gene flow. Coalescent analysis of gene flow between
 the two Joshua tree types suggests that it may be shaped by asymmetric
 pollinator specificity, which has been observed in a narrow zone of
 sympatry. Finally, we find evidence suggesting that gene flow among Joshua
 tree sites may shape floral morphology within one plant-pollinator
 association, but not the other.
description: Collection sites and
 sample sizesSummary table of field
 sites with latitude (lat_dec) and longitude (lon_dec), sorting order for
 Table 1 and Figure 1c (sort), and sample sizes for Joshua tree genetics
 (JotrGen) and phenotype measurements (JotrPhen) and moth genetics
 (MothGen) and phenotypes (MothPhen).Yoder_etal_sites.txtJoshua tree genotypesDiploid genotypes at ten microsatellite loci for Joshua tree, given as PCR fragment size.Yoder_etal_Ybrev-genotypes.txtTegeticula synthetica genotypesDiploid genotypes at nine microsatellite loci for Tegeticula synthetica, given as motif repeat counts.Yoder_etal_Tsyn-genotypes.txtTegeticula antithetica genotypesDiploid genotypes at nine microsatellite loci for Tegeticula antithetica, given as motif repeat counts.Yoder_etal_Tant-genotypes.txtJoshua tree phenotypic measurementsPhenotypic measurements for Joshua tree. Includes measurements not analyzed in the present manuscript: crown diameter in meters (crown dia); branch count (branch #); lengths in centimeters and widths in millimeters for five leaves (l1, w1, l2, w2, l3, w3, l4, w4, l5, w5); total tree height in meters (tree_h); height to first branching in meters (h_1st_br); petal length and width in mm (pet_l and pet_w); diagonal length of a petal in mm (pet_diag); total pistil length and width in mm (pis_l and pis_w); style length, basal width, and midpoint width in mm (sty_l, sty_bw, sty_mw).Yoder_etal_floral.txtPollinator ovipositor lengthsOvipositor measurements in millimeters (ovip_mm) for both species of moths pollinating Joshua tree. Date of preparation and imaging/measurement, and the initials of observer are given for each specimen.Yoder_etal_ovip-lengths.txt