DOI: 10.5061/dryad.84gf5
title: Data from: Local adaptation and the evolution of phenotypic plasticity in Trinidadian guppies (Poecilia reticulata)

description: Divergent selection pressures across environments can result in phenotypic
 differentiation that is due to local adaptation, phenotypic plasticity, or
 both. Trinidadian guppies exhibit local adaptation to the presence or
 absence of predators, but the degree to which predator-induced plasticity
 contributes to population differentiation is less clear. We conducted
 common garden experiments on guppies obtained from two drainages
 containing populations adapted to high- and low-predation environments. We
 reared full-sibs from all populations in treatments simulating the
 presumed ancestral (predator-cues present) and derived (predator-cues
 absent) conditions and measured water column use, head morphology, and
 size at maturity. When reared in presence of predator cues, all
 populations had phenotypes that were typical of a high-predation ecotype.
 However, when reared in the absence of predator cues, guppies from high-
 and low-predation regimes differed in head morphology and size at
 maturity; the qualitative nature of these differences corresponded to
 those that characterize adaptive phenotypes in high- versus low-predation
 environments. Thus, divergence in plasticity is due to phenotypic
 differences between high- and low-predation populations when reared in the
 absence of predator cues. These results suggest that plasticity might
 initially play an important role during colonization of novel
 environments, and then evolve as a by-product of adaptation to the derived
 environment.
description: Torres Dowdall et al.
 11-0833 Standard Length dataData use in the analysis
 of size at maturity. Data correspond to size at maturity of lab-reared
 male guppies (Poecilia reticulata). Size was measured as standard length
 of the fish (length column). Drainage refer to the drainage from were wild
 fish used to generate second generation of lab-reared experimental fish
 were collected. Pred refers to the community from which the wild caught
 fish were collected. LP represent low predation risk, simple fish
 communities. HP refer to downstream complex communities where predation
 risk is higher. Family refers to the family lines used in the experiment.
 Cue refers to the two treatments used in our experiment. hp means that
 fish were reared in the presence of predator cues, lp means that they were
 raised in the absence of such cues. Only significant interactions between
 terms were kept in the final model.Torres Dowdall et al.
 11-0833 Water colum dataData use in the analysis
 of water column use. Data correspond to proportion of guppies (Poecilia
 reticulata) using the surface of the water column. Drainage refer to the
 drainage from were wild fish used to generate second generation of
 lab-reared experimental fish were collected. Predation refers to the
 community from which the wild caught fish were collected. "low"
 represent low predation risk, simple fish communities. "High"
 refer to downstream complex communities where predation risk is higher.
 Family refers to the family lines used in the experiment. "trt"
 refers to the two treatments used in our experiment. "P" means
 that fish were reared in the presence of predator cues, "NP"
 means that they were raised in the absence of such cues. Only significant
 interactions between terms were kept in the final
 model.Torres Dowdall et al.
 11-0833 Procrustes coordinates data for Yarra fishData used in the
 analysis of head morphology from the Yarra Drainage. This text file
 contains the Procrustes Coordinates that were used for all multivariate
 analyses of morphology in the manuscript. The Procrustes Coordinates were
 produced by performing a Generalized Procrustes Analysis (GPA) on the
 original Cartesian Coordinates to remove size, orientation, and position
 effects from the data with the TPSRelw software packackage. Additionally,
 sliding semi-landmarks were aligned during the GPA with the TPSRelw
 software. Columns are as follow: “Id” refers to the individual assigned
 identification. Family refers to the family lines used in the experiment.
 “Predation” refers to the community from which the wild caught fish were
 collected; "low" represent low predation risk, simple fish
 communities; "High" refer to downstream complex communities
 where predation risk is higher. "Treatment" refers to the two
 treatments used in our experiment. "Cue" means that fish were
 reared in the presence of predator cues, "No Cue" means that
 they were raised in the absence of such cues. Drainage refer to the
 drainage from were wild fish used to generate second generation of
 lab-reared experimental fish were collected. Population refers to the
 interaction between Drainage and Predation. Data was analyzed in MANCOVA
 model combining both drainages, and later in separate
 CVAs.Torres Dowdall et al.
 11-0833 Procrustes coordinates data for Guanapo fishData used in the
 analysis of head morphology from the Guanapo Drainage. This text file
 contains the Procrustes Coordinates that were used for all multivariate
 analyses of morphology in the manuscript. The Procrustes Coordinates were
 produced by performing a Generalized Procrustes Analysis (GPA) on the
 original Cartesian Coordinates to remove size, orientation, and position
 effects from the data with the TPSRelw software package. Additionally,
 sliding semi-landmarks were aligned during the GPA with the TPSRelw
 software. Columns are as follow: “Id” refers to the individual assigned
 identification. Family refers to the family lines used in the experiment.
 “Predation” refers to the community from which the wild caught fish were
 collected; "low" represent low predation risk, simple fish
 communities; "High" refer to downstream complex communities
 where predation risk is higher. "Treatment" refers to the two
 treatments used in our experiment. "Cue" means that fish were
 reared in the presence of predator cues, "No Cue" means that
 they were raised in the absence of such cues. Drainage refer to the
 drainage from were wild fish used to generate second generation of
 lab-reared experimental fish were collected. Population refers to the
 interaction between Drainage and Predation. Data was analyzed in MANCOVA
 model combining both drainages, and later in separate
 CVAs.