DOI: 10.5061/dryad.5md80
title: Data from: Nutrient distribution and absorption in the colonial hydroid Podocoryna carnea is sequentially diffusive and directional

description: The distribution and absorption of ingested protein was characterized
 within a colony of Podocoryna carnea when a single polyp was fed.
 Observations were conducted at multiple spatial and temporal scales at
 three different stages of colony ontogeny with an artificial food item
 containing Texas Red conjugated albumin. Food pellets were digested and
 all tracer absorbed by digestive cells within the first 2–3 hours
 post-feeding. The preponderance of the label was located in the fed polyp
 and in a transport-induced diffusion pattern surrounding the fed polyp.
 After 6 hours post-feeding particulates re-appeared in the gastrovascular
 system and their absorption increased the area over which the nutrients
 were distributed, albeit still in a pattern that was centered on the fed
 polyp. At later intervals, tracer became concentrated in some stolon tips,
 but not in others, despite the proximity of these stolons either to the
 fed polyp or to adjacent stolons receiving nutrients. Distribution and
 absorption of nutrients is sequentially diffusive and directional.
description: B1_Replicate Early
 Ontogeny_Oct 1Digitized images
 documenting colony morphology for replicate films. Scale bar = 1mm. Blue
 open circle corresponds to size and location of fed polyp. Red open
 circles correspond to size and locations of unfed
 stolons.B2_Replicate
 Mid-Ontogeny_2 JulDigitized images
 documenting colony morphology for replicate films. Scale bar = 1mm. Blue
 open circle corresponds to size and location of fed polyp. Red open
 circles correspond to size and locations of unfed
 stolons.B3 Replicate_Late
 Ontogeny_March 19Digitized images
 documenting colony morphology for replicate films. Scale bar = 1mm. Blue
 open circle corresponds to size and location of fed polyp. Red open
 circles correspond to size and locations of unfed
 stolons.B4_Replicate_Medusae-bearing Colony_8 JulyDigitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.B5_Replicate Stolon Growth_20 JunDigitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.A1 Early ontogeny_15 Oct_ShortEarly ontogeny colony_15 Oct_Short First image at 22 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 1 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 6.74 µm/pixel.A2 Early ontogeny_15 Oct_LongEarly ontogeny colony_15 Oct_Long First image at 22 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 4 sec. The long exposure was used to quantify the luminosity of the unfed polyps and hydrorhiza. Scale: 6.74 µm/pixel.A3 Mid-ontogeny colony_22 JulyMid-ontogeny colony_22 Jul First image at 43 minutes post-feeding, subsequent images at 3 hour intervals. Exposure = 5 sec. Scale: 2.4 µm/pixel.A4 Late ontogeny_12 June_ShortLate ontogeny colony_12 June 2014_Short 1.3 First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 0.5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel.A5 Late ontogeny_12 June_Long_pt 1Late ontogeny colony_12 June 2014_Long First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.A5 Late ontogeny_12 June_Long_pt 2Late ontogeny colony_12 June 2014_Long_pt 2 First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.A6 Medusa-bearing Colony_3 Oct_ShortMedusae-bearing colony_3 Oct_Short First image at 21 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 0.5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 3.6 µm/pixel.A7 Medusa-bearing Colony_3 Oct_Long_pt 1Medusae-bearing colony_3 Oct_Long_pt 1 First image at 21 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 2 sec. The long exposure was used to quantify the luminosity of the unfed polyps and hydrorhiza. Scale: 3.6 µm/pixel. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.A7 Medusa-bearing Colony_3 Oct_Long_pt 2Medusae-bearing colony_3 Oct_Long_pt 2 First image at 21 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 2 sec. The long exposure was used to quantify the luminosity of the unfed polyps and hydrorhiza. Scale: 3.6 µm/pixel. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.A8 Cut Stolon study_1 MarchCut stolon experiment_1 Mar First image at 35 minutes post-feeding, subsequent images at 1 hour intervals. Exposure for frames 1-5 = 4 sec, exposure for remaining frames = 8 sec. Scale: 3.26 µm/pixel.A9 Fed Polyp_10 SeptFed Polyp_10 Sept First image at 15 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 4 sec. Scale: 3.08 µm/pixel.C1 Replicate Early ontogeny_1 Oct_ShortReplicate_Early Ontogeny_1 Oct_Short First image at 25 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 0.5 sec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.C2 Replicate_Early ontogeny_1 Oct_LongReplicate_Early Ontogeny_1 Oct_ Long First image at 25 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 2 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza.C3 Replicate_Mid Ontogeny_2 July_shortReplicate_Mid-Ontogeny_2 Jul_Short First image at 24 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 0.5 sec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.C4 Replicate_Mid Ontogeny_2 July_longReplicate_Mid-Ontogeny_2 Jul_Long First image at 24 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza.C5 Replicate_Late ontogeny_19 March_ShortReplicate_Late Ontogeny_19 Mar_Short First image at 35 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 50 msec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.C6 Replicate_Late ontogeny_19 March_Long_pt 1Replicate_Late Ontogeny_19 Mar_Long_pt 1 First image at 35 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.C6 Replicate_Late ontogeny_19 March_Long_pt 2Replicate_Late Ontogeny_19 Mar_Long_pt 2 First image at 35 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.C7 Replicate_Medusa-bearing_8 July_ShortReplicate_Medusae-bearing_ 8 Jul_Short First image at 19 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 0.5 sec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.C8 Replicate_Medusa-bearing_8 July_Long_pt 1Replicate_Medusae-bearing_ 8 Jul_Long_part 1 First image at 19 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.C8 Replicate_Medusa-bearing_8 July_Long_pt 2Replicate_Medusae-bearing_ 8 Jul_Long_part 2 First image at 19 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.C9 Replicate_Stolon Growth_20 JuneReplicate_ Stolon Growth_20 Jun First image at 27 minutes post-feeding, subsequent images at 3 hour intervals. Exposure = 0.45 sec for frames 1-8, 4 sec for frames 9-24. sec. Scale: 2.45 µm/pixel.C10 Replicate_Fed Polyp_17 SeptReplicate_Fed Polyp_17 Sept First image at 20 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 0.75 sec. Scale: 3.27 µm/pixel.