cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5668720.v1
title: Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration

description: Abstract Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p&lt;0.05) increased cell proliferation, except for BMSC grown on non-conditioned dentin slices. Dentin substrate discretely increased expression of Col1a1 in all cell types. Expression of Runx2, Col1a1 and Fn was either unaffected or inhibited by bFGF application in all cell types. We could not detect expression of the target genes on BMSC grown onto conditioned dentin. Conclusion: Acid conditioning of dentin improves the release of topically-applied bFGF. Topical application of bFGF had a stimulatory effect on proliferation of PDL fibroblasts, cementoblasts and BMSC, but did not affect expression of Runx2, Col1a1, Bglap and fibronectin by these cells.
DOI: 10.6084/m9.figshare.5668717
title: Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride

description: Abstract Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2: 1% MC/CaCl2; Group 3: 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (p
DOI: 10.6084/m9.figshare.5668717.v1
title: Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride

description: Abstract Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2: 1% MC/CaCl2; Group 3: 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (p
DOI: 10.6084/m9.figshare.5668705
title: Participatory environmental diagnosis and of health risks from the surrounding communities the Petrochemical Complex of Rio de Janeiro, Brazil

description: Abstract The aim of this study was to diagnose the priority socio environmental problems and the health risks from the surrounding communities the Petrochemical Complex of Rio de Janeiro. Characterized by a participatory approach, the action research has led to the application of interviews, focal groups, meetings and workshop with social actors of Porto das Caixas and Sambaetiba districts, located in Itaboraí city/RJ from November 2013 to December 2014. A structural analysis of the problems prioritized by the communities (water supply, sewage treatment and risk of transmissible diseases; risk of air pollution and respiratory diseases; absence of public security and risk of violence) sketched out the cause-effect-intervention relationship, on the basis of the Protocol for Assessing Community Excellence in Environmental Health. The process revealed the absence of representativity of the social actors of the studied localities in spaces of decision-making on the environmental issue. Educational actions with professionals and inhabitants that aim to promote the formation of collective movements urge, indispensable to guarantee the rights of mitigation of situations of contamination of air and access to sanitation services and public security and thus of conditions of lower risk to health.
DOI: 10.25345/c53f32
title: MassIVE MSV000086041 - Glycoproteoform profiles of individual patients derived plasma alpha-1-antichymotrypsin are unique and extensively remodeled in response to a septic episode
title: Proteomics analysis of human plasma from ten independent sepsis patients with four time points.
DOI: 10.6084/m9.figshare.5668705.v1
title: Participatory environmental diagnosis and of health risks from the surrounding communities the Petrochemical Complex of Rio de Janeiro, Brazil

description: Abstract The aim of this study was to diagnose the priority socio environmental problems and the health risks from the surrounding communities the Petrochemical Complex of Rio de Janeiro. Characterized by a participatory approach, the action research has led to the application of interviews, focal groups, meetings and workshop with social actors of Porto das Caixas and Sambaetiba districts, located in Itaboraí city/RJ from November 2013 to December 2014. A structural analysis of the problems prioritized by the communities (water supply, sewage treatment and risk of transmissible diseases; risk of air pollution and respiratory diseases; absence of public security and risk of violence) sketched out the cause-effect-intervention relationship, on the basis of the Protocol for Assessing Community Excellence in Environmental Health. The process revealed the absence of representativity of the social actors of the studied localities in spaces of decision-making on the environmental issue. Educational actions with professionals and inhabitants that aim to promote the formation of collective movements urge, indispensable to guarantee the rights of mitigation of situations of contamination of air and access to sanitation services and public security and thus of conditions of lower risk to health.
DOI: 10.6084/m9.figshare.5668702
title: Characterization of Impact Toughness Properties of DIN39MnCrB6-2 Steel Grade

description: Boron added steels in quenched and tempered condition have been widely used in applications where good mechanical properties and low cost are required. In this study, impact resistance of DIN 39MnCrB6-2 steel grade was investigated due to its criticality in automotive components. Metastable phase diagrams were built to guide heat treatments. The influence heat treatment parameters and initial microstructure were evaluated. Impact toughness results showed low absorbed energy (&lt;30J) for all tempering temperatures. CVN impact toughness was substantially increased in samples submitted to solution treatment. Microscopic observation showed coarse borocarbides in surface fracture of Q&T samples. It was concluded that the formation of borocarbides is inherent in boron steels and their coarse morphology should be avoided in order to reduce embrittlement.
DOI: 10.6084/m9.figshare.5668702.v1
title: Characterization of Impact Toughness Properties of DIN39MnCrB6-2 Steel Grade

description: Boron added steels in quenched and tempered condition have been widely used in applications where good mechanical properties and low cost are required. In this study, impact resistance of DIN 39MnCrB6-2 steel grade was investigated due to its criticality in automotive components. Metastable phase diagrams were built to guide heat treatments. The influence heat treatment parameters and initial microstructure were evaluated. Impact toughness results showed low absorbed energy (&lt;30J) for all tempering temperatures. CVN impact toughness was substantially increased in samples submitted to solution treatment. Microscopic observation showed coarse borocarbides in surface fracture of Q&T samples. It was concluded that the formation of borocarbides is inherent in boron steels and their coarse morphology should be avoided in order to reduce embrittlement.
DOI: 10.6084/m9.figshare.5668699
title: Pre-sintered Y-TZP sandblasting: effect on surface roughness, phase transformation, and Y-TZP/veneer bond strength

description: Abstract Sandblasting is a common method to try to improve the Y-TZP/veneer bond strength of dental prostheses, however, it may put stress on zirconia surfaces and could accelerate the t→m phase transformation. Y-TZP sandblasting before sintering could be an alternative to improve surface roughness and bonding strength of veneering ceramic. Objectives. The aim of this study was to analyze the effect of Y-TZP pre-sintering sandblasting on surface roughness, phase transformation, and the Y-TZP/veneer shear bond strength. Material and Methods. The Y-TZP specimen surface underwent sandblasting with aluminum oxide (50 μm) pre-sintering (Z-PRE) and post-sintering (Z-POS). Z-CTR was not subjected to surface treatment. After ceramic veneer application, the specimens were subjected to shear bond testing. Surface roughness was analyzed by confocal microscopy. Y-TZP monoclinic and tetragonal phases were evaluated by micro-Raman spectroscopy. Shear bond strength and surface roughness data were analyzed by One-way ANOVA and Tukey tests (α=0.05). Differences in the wave numbers and the broadening bands of the Raman spectra were compared among groups. Results. Z-POS (9.73±5.36 MPa) and Z-PRE (7.94±2.52 MPa) showed the highest bond strength, significantly higher than that of Z-CTR (5.54±2.14 MPa). The Ra of Z-PRE (1.59±0.23 µm) was much greater and significantly different from that of Z-CTR (0.29±0.05 µm) and Z-POS (0.77±0.13 µm). All groups showed bands typical of the tetragonal (T) and monoclinic (M) phases. Y-TZP sandblasting before sintering resulted in rougher surfaces but did not increase the shear bond strength compared to post-sintering and increased surface defects. Conclusions. Surface treatment with Al3O2, regardless of the moment and application, improves the results of Y-TZP/veneer bonding and is not a specific cause of t→m transformation.
DOI: 10.6084/m9.figshare.5668699.v1
title: Pre-sintered Y-TZP sandblasting: effect on surface roughness, phase transformation, and Y-TZP/veneer bond strength

description: Abstract Sandblasting is a common method to try to improve the Y-TZP/veneer bond strength of dental prostheses, however, it may put stress on zirconia surfaces and could accelerate the t→m phase transformation. Y-TZP sandblasting before sintering could be an alternative to improve surface roughness and bonding strength of veneering ceramic. Objectives. The aim of this study was to analyze the effect of Y-TZP pre-sintering sandblasting on surface roughness, phase transformation, and the Y-TZP/veneer shear bond strength. Material and Methods. The Y-TZP specimen surface underwent sandblasting with aluminum oxide (50 μm) pre-sintering (Z-PRE) and post-sintering (Z-POS). Z-CTR was not subjected to surface treatment. After ceramic veneer application, the specimens were subjected to shear bond testing. Surface roughness was analyzed by confocal microscopy. Y-TZP monoclinic and tetragonal phases were evaluated by micro-Raman spectroscopy. Shear bond strength and surface roughness data were analyzed by One-way ANOVA and Tukey tests (α=0.05). Differences in the wave numbers and the broadening bands of the Raman spectra were compared among groups. Results. Z-POS (9.73±5.36 MPa) and Z-PRE (7.94±2.52 MPa) showed the highest bond strength, significantly higher than that of Z-CTR (5.54±2.14 MPa). The Ra of Z-PRE (1.59±0.23 µm) was much greater and significantly different from that of Z-CTR (0.29±0.05 µm) and Z-POS (0.77±0.13 µm). All groups showed bands typical of the tetragonal (T) and monoclinic (M) phases. Y-TZP sandblasting before sintering resulted in rougher surfaces but did not increase the shear bond strength compared to post-sintering and increased surface defects. Conclusions. Surface treatment with Al3O2, regardless of the moment and application, improves the results of Y-TZP/veneer bonding and is not a specific cause of t→m transformation.
DOI: 10.6084/m9.figshare.5668696
title: Influence of curing protocol and ceramic composition on the degree of conversion of resin cement

description: Abstract Due to increasing of aesthetic demand, ceramic crowns are widely used in different situations. However, to obtain long-term prognosis of restorations, a good conversion of resin cement is necessary. Objective: To evaluate the degree of conversion (DC) of one light-cure and two dual-cure resin cements under a simulated clinical cementation of ceramic crowns. Material and Methods: Prepared teeth were randomly split according to the ceramic's material, resin cement and curing protocol. The crowns were cemented as per manufacturer's directions and photoactivated either from occlusal suface only for 60 s; or from the buccal, occlusal and lingual surfaces, with an exposure time of 20 s on each aspect. After cementation, the specimens were stored in deionized water at 37°C for 7 days. Specimens were transversally sectioned from occlusal to cervical surfaces and the DC was determined along the cement line with three measurements taken and averaged from the buccal, lingual and approximal aspects using micro-Raman spectroscopy (Alpha 300R/WITec®). Data were analyzed by 3-way ANOVA and Tukey test at =5%. Results: Statistical analysis showed significant differences among cements, curing protocols and ceramic type (p&lt;0.001). The curing protocol 3x20 resulted in higher DC for all tested conditions; lower DC was observed for Zr ceramic crowns; Duolink resin cement culminated in higher DC regardless ceramic composition and curing protocol. Conclusion: The DC of resin cement layers was dependent on the curing protocol and type of ceramic.
DOI: 10.6084/m9.figshare.5668696.v1
title: Influence of curing protocol and ceramic composition on the degree of conversion of resin cement

description: Abstract Due to increasing of aesthetic demand, ceramic crowns are widely used in different situations. However, to obtain long-term prognosis of restorations, a good conversion of resin cement is necessary. Objective: To evaluate the degree of conversion (DC) of one light-cure and two dual-cure resin cements under a simulated clinical cementation of ceramic crowns. Material and Methods: Prepared teeth were randomly split according to the ceramic's material, resin cement and curing protocol. The crowns were cemented as per manufacturer's directions and photoactivated either from occlusal suface only for 60 s; or from the buccal, occlusal and lingual surfaces, with an exposure time of 20 s on each aspect. After cementation, the specimens were stored in deionized water at 37°C for 7 days. Specimens were transversally sectioned from occlusal to cervical surfaces and the DC was determined along the cement line with three measurements taken and averaged from the buccal, lingual and approximal aspects using micro-Raman spectroscopy (Alpha 300R/WITec®). Data were analyzed by 3-way ANOVA and Tukey test at =5%. Results: Statistical analysis showed significant differences among cements, curing protocols and ceramic type (p&lt;0.001). The curing protocol 3x20 resulted in higher DC for all tested conditions; lower DC was observed for Zr ceramic crowns; Duolink resin cement culminated in higher DC regardless ceramic composition and curing protocol. Conclusion: The DC of resin cement layers was dependent on the curing protocol and type of ceramic.
DOI: 10.6084/m9.figshare.5668693
title: Cardiovascular emergencies in primary care: an observational retrospective study of a large-scale telecardiology service

description: ABSTRACT BACKGROUND: Electrocardiograms (ECGs) are an essential examination for identification and management of cardiovascular emergencies.The aim of this study was to report on the frequency and recognition of cardiovascular emergencies in primary care units. DESIGN AND SETTING: Observational retrospective study assessing consecutive patients whose digital ECGs were sent for analysis to the team of the Telehealth Network of Minas Gerais. METHODS: Data from patients diagnosed with cardiological emergencies in the primary care setting of 750 municipalities in Minas Gerais, Brazil, between March and September 2015, were collected via telephone contact with the healthcare practitioner who performed the ECG. After collection, the data were subjected to statistical analysis. RESULTS: Over the study period, 304 patients with cardiovascular emergencies were diagnosed within primary care. Only 73.4% of these were recognized by the local physicians. Overall, the most frequent ECG abnormalities were acute ischemic patterns (44.7%) and the frequency of such patterns was higher among the ECGs assigned as emergency priority (P = 0.03). It was possible to obtain complete information on 231 patients (75.9%). Among these, the mean age was 65 ± 14.4 years, 57.1% were men and the most prevalent comorbidity was hypertension (68.4%). In total, 77.9% were referred to a unit caring for cases of higher complexity and 11.7% of the patients died. CONCLUSION: In this study, cardiovascular emergencies were misdiagnosed in primary care settings, acute myocardial ischemia was the most frequent emergency and the mortality rate was high.
DOI: 10.6084/m9.figshare.5668693.v1
title: Cardiovascular emergencies in primary care: an observational retrospective study of a large-scale telecardiology service

description: ABSTRACT BACKGROUND: Electrocardiograms (ECGs) are an essential examination for identification and management of cardiovascular emergencies.The aim of this study was to report on the frequency and recognition of cardiovascular emergencies in primary care units. DESIGN AND SETTING: Observational retrospective study assessing consecutive patients whose digital ECGs were sent for analysis to the team of the Telehealth Network of Minas Gerais. METHODS: Data from patients diagnosed with cardiological emergencies in the primary care setting of 750 municipalities in Minas Gerais, Brazil, between March and September 2015, were collected via telephone contact with the healthcare practitioner who performed the ECG. After collection, the data were subjected to statistical analysis. RESULTS: Over the study period, 304 patients with cardiovascular emergencies were diagnosed within primary care. Only 73.4% of these were recognized by the local physicians. Overall, the most frequent ECG abnormalities were acute ischemic patterns (44.7%) and the frequency of such patterns was higher among the ECGs assigned as emergency priority (P = 0.03). It was possible to obtain complete information on 231 patients (75.9%). Among these, the mean age was 65 ± 14.4 years, 57.1% were men and the most prevalent comorbidity was hypertension (68.4%). In total, 77.9% were referred to a unit caring for cases of higher complexity and 11.7% of the patients died. CONCLUSION: In this study, cardiovascular emergencies were misdiagnosed in primary care settings, acute myocardial ischemia was the most frequent emergency and the mortality rate was high.
DOI: 10.6084/m9.figshare.5668690
title: Perception of hearing loss: use of the subjective faces scale to screen hearing among the elderly

description: Abstract Presbycusis is a disorder present among the elderly. However, it is under-diagnosed, making it important to develop and enhance simple screening tools. Objective: The subjective faces scale has been proposed as a method to assess auditory self-perception among the elderly, and its correlation with audiological tests. Methods: We looked at elderly patients referred to the audiology service of a reference center for the care of the elderly in a public university hospital between February and November 2013. Patients were submitted to meatoscopy, tonal and vocal audiometry and the whisper test. They also answered the subjective faces scale. A total of 164 elderly individuals participated, and the average age was 77. Results: We found a good correlation between the subjective faces scale and audiometry thresholds (r = 0.66). Our results show that the faces and hearing loss correlate, with face 1 corresponding to normal hearing, face 2 to mild hearing loss, and face 3 to Grade I moderate hearing loss. When evaluating the psychometric qualities of the subjective faces scale, we found that faces 2 or 3 have good sensitivity and specificity, with the area under the ROC curve being 0.81. Conclusion: The subjective faces scale seems to be a good, low-cost and easy to use supplementary tool for auditory screening in geriatric services.
DOI: 10.6084/m9.figshare.5668690.v1
title: Perception of hearing loss: use of the subjective faces scale to screen hearing among the elderly

description: Abstract Presbycusis is a disorder present among the elderly. However, it is under-diagnosed, making it important to develop and enhance simple screening tools. Objective: The subjective faces scale has been proposed as a method to assess auditory self-perception among the elderly, and its correlation with audiological tests. Methods: We looked at elderly patients referred to the audiology service of a reference center for the care of the elderly in a public university hospital between February and November 2013. Patients were submitted to meatoscopy, tonal and vocal audiometry and the whisper test. They also answered the subjective faces scale. A total of 164 elderly individuals participated, and the average age was 77. Results: We found a good correlation between the subjective faces scale and audiometry thresholds (r = 0.66). Our results show that the faces and hearing loss correlate, with face 1 corresponding to normal hearing, face 2 to mild hearing loss, and face 3 to Grade I moderate hearing loss. When evaluating the psychometric qualities of the subjective faces scale, we found that faces 2 or 3 have good sensitivity and specificity, with the area under the ROC curve being 0.81. Conclusion: The subjective faces scale seems to be a good, low-cost and easy to use supplementary tool for auditory screening in geriatric services.