cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.5718397
title: INTERDISCIPLINARY AND INTERPROFESSIONALITY IN THE FAMILY HEALTH STRATEGY

description: Abstract Interdisciplinarity and its practical correlation, interprofessionality, are essential to the work in the Family Health Strategy. The objective was to characterize the interdisciplinary perspective of Family Health Strategy workers in a Brazilian capital, linking it to interprofessionality. Transversal, exploratory and descriptive study, developed from the qualitative and quantitative approaches. Data were collected from june to november 2014 through questionnaires and participant observation. The results of the questionnaire were converted into scores, allowing to classify the level of interdisciplinarity. The observation findings, recorded in the field diary, were submitted to content analysis and discussed in parallel with the quantitative findings. It was verified, in the questionnaires, that the dimension ‘reception’ was the only one that presented medium availability for practices that can make interdisciplinarity feasible. The results indicate that the health professionals’ perception about interdisciplinarity was positive, however, contradictions of these findings with the ones observed locus . In the participant observation, it was verified that the professionals need to follow some paths in the sense of materializing the interdisciplinarity in collaborative interprofessional practices. It is important to emphasize the need for management-level actions that favor these practices; Permanent education as a strategy for coping with integration difficulties; And the subjective investment of workers in the same direction.
DOI: 10.6084/m9.figshare.5718394
title: In Situ Synchrotron Radiation Measurements During Axial Strain In Hydrogen Cathodically Charged Duplex Stainless Steel SAF 2205

description: The objective of this work is the evaluation of hydrogen effects on the martensitic transformation and strain hardening in Duplex Stainless Steels (DSS) SAF 2205 (UNS S32205/S31803). DSS are two-phase alloys (austenite and ferrite), which are used for applications requiring high mechanical strength, in corrosive environments. Therefore, it is necessary a better understanding of the phenomena involved on the hydrogen embrittlement. For this, in situ measurements of X-ray diffraction were made during tensile test in H2 cathodically charging DSS 2205. The hydrogen charging reduces the stress relaxation, reducing the ductility and suppressing the hydrogen-induced austenitic to martensitic transformation. In addition, it also reduces the strain hardening (dislocation multiplication) in austenite. The strain hardening seems to have a higher influence than martensitic transformation on fracture process, even in absence of hydrogen.
DOI: 10.6084/m9.figshare.5718397.v1
title: INTERDISCIPLINARY AND INTERPROFESSIONALITY IN THE FAMILY HEALTH STRATEGY

description: Abstract Interdisciplinarity and its practical correlation, interprofessionality, are essential to the work in the Family Health Strategy. The objective was to characterize the interdisciplinary perspective of Family Health Strategy workers in a Brazilian capital, linking it to interprofessionality. Transversal, exploratory and descriptive study, developed from the qualitative and quantitative approaches. Data were collected from june to november 2014 through questionnaires and participant observation. The results of the questionnaire were converted into scores, allowing to classify the level of interdisciplinarity. The observation findings, recorded in the field diary, were submitted to content analysis and discussed in parallel with the quantitative findings. It was verified, in the questionnaires, that the dimension ‘reception’ was the only one that presented medium availability for practices that can make interdisciplinarity feasible. The results indicate that the health professionals’ perception about interdisciplinarity was positive, however, contradictions of these findings with the ones observed locus . In the participant observation, it was verified that the professionals need to follow some paths in the sense of materializing the interdisciplinarity in collaborative interprofessional practices. It is important to emphasize the need for management-level actions that favor these practices; Permanent education as a strategy for coping with integration difficulties; And the subjective investment of workers in the same direction.
DOI: 10.6084/m9.figshare.5718394.v1
title: In Situ Synchrotron Radiation Measurements During Axial Strain In Hydrogen Cathodically Charged Duplex Stainless Steel SAF 2205

description: The objective of this work is the evaluation of hydrogen effects on the martensitic transformation and strain hardening in Duplex Stainless Steels (DSS) SAF 2205 (UNS S32205/S31803). DSS are two-phase alloys (austenite and ferrite), which are used for applications requiring high mechanical strength, in corrosive environments. Therefore, it is necessary a better understanding of the phenomena involved on the hydrogen embrittlement. For this, in situ measurements of X-ray diffraction were made during tensile test in H2 cathodically charging DSS 2205. The hydrogen charging reduces the stress relaxation, reducing the ductility and suppressing the hydrogen-induced austenitic to martensitic transformation. In addition, it also reduces the strain hardening (dislocation multiplication) in austenite. The strain hardening seems to have a higher influence than martensitic transformation on fracture process, even in absence of hydrogen.
DOI: 10.6084/m9.figshare.5718391
title: Effect of CRABP2 on the proliferation and odontoblastic differentiation of hDPSCs

description: Abstract: Cellular retinoic acid-binding protein 2 (CRABP2) has been detected in several organs during embryonic development. Recent studies have demonstrated that CRABP2 plays important roles in the retinoic acid, β-catenin and Notch signaling pathways, as well as in the interaction between epithelial and mesenchymal cells, which are important for human dental pulp stem cells (hDPSCs) and tooth development. In the present study, the expression of CRABP2 during mouse molar development and the role of CRABP2 in hDPSC odontoblastic differentiation were evaluated. CRABP2 was gradually decreased during the development of the first maxillary molar, which exhibited the same trend as the expression of CRABP2 during the odontoblastic induction of hDPSCs. CRABP2 knockdown inhibited the proliferative ability of hDPSCs, while it enhanced odontoblastic differentiation via promoting mineralization nodule formation and upregulating the activity of alkaline phosphatase and the expression of mineralization-related genes. The present study uncovered a novel function of CRABP2 in hDPSCs. Our data suggest that CRABP2 may act as a regulator during the proliferation and differentiation of hDPSCs.
DOI: 10.6084/m9.figshare.5718391.v1
title: Effect of CRABP2 on the proliferation and odontoblastic differentiation of hDPSCs

description: Abstract: Cellular retinoic acid-binding protein 2 (CRABP2) has been detected in several organs during embryonic development. Recent studies have demonstrated that CRABP2 plays important roles in the retinoic acid, β-catenin and Notch signaling pathways, as well as in the interaction between epithelial and mesenchymal cells, which are important for human dental pulp stem cells (hDPSCs) and tooth development. In the present study, the expression of CRABP2 during mouse molar development and the role of CRABP2 in hDPSC odontoblastic differentiation were evaluated. CRABP2 was gradually decreased during the development of the first maxillary molar, which exhibited the same trend as the expression of CRABP2 during the odontoblastic induction of hDPSCs. CRABP2 knockdown inhibited the proliferative ability of hDPSCs, while it enhanced odontoblastic differentiation via promoting mineralization nodule formation and upregulating the activity of alkaline phosphatase and the expression of mineralization-related genes. The present study uncovered a novel function of CRABP2 in hDPSCs. Our data suggest that CRABP2 may act as a regulator during the proliferation and differentiation of hDPSCs.
DOI: 10.6084/m9.figshare.5718388
title: Microtensile bond strength of CAD/CAM materials to dentin under different adhesive strategies

description: Abstract: The present study aimed to evaluate the microtensile bond strength (μTBS) of novel computer-aided design/computer-aided manufacturing (CAD/CAM) restorative materials to dentin using different adhesive strategies. Thirty-two crowns were milled using CAD/CAM materials (Vita Mark II as control, Vita Suprinity, Vita Enamic and Lava Ultimate) and luted to dentin using different resin cements (RelyX ARC, RelyX Unicem 2 and RelyX Ultimate). The specimens were stored in 100% relative humidity at 37°C for 24 h and sectioned. The samples (n = 16) with cross-sectional areas of approximately 0.90 mm2, were submitted to a μTBS test in a universal testing machine with a crosshead speed of 0.5 mm/min. The samples were analyzed with SEM to determinate the failure mode. According to 2-way ANOVA and Tukey's test (α=0.05), the interaction effect (material x luting strategy) was significant (p=0.001). Regardless of the luting strategy, a higher μTBS was obtained with Lava Ultimate and Vita Enamic, which were significantly different from Vita Mark II and Vita Suprinity (p&lt;0.05). For Vita Mark II, Vita Suprinity, Vita Enamic, and Lava Ultimate, the μTBS obtained with RelyX Unicem 2 was not significantly different from that obtained with RelyX ARC or RelyX Ultimate. All groups obtained at least three types of failure. The adhesive strategy with self-adhesive resin cement was comparable to conventional resin cement with total-etch or self-etch adhesive techniques in the bond of novel CAD/CAM materials to dentin.
DOI: 10.6084/m9.figshare.5718388.v1
title: Microtensile bond strength of CAD/CAM materials to dentin under different adhesive strategies

description: Abstract: The present study aimed to evaluate the microtensile bond strength (μTBS) of novel computer-aided design/computer-aided manufacturing (CAD/CAM) restorative materials to dentin using different adhesive strategies. Thirty-two crowns were milled using CAD/CAM materials (Vita Mark II as control, Vita Suprinity, Vita Enamic and Lava Ultimate) and luted to dentin using different resin cements (RelyX ARC, RelyX Unicem 2 and RelyX Ultimate). The specimens were stored in 100% relative humidity at 37°C for 24 h and sectioned. The samples (n = 16) with cross-sectional areas of approximately 0.90 mm2, were submitted to a μTBS test in a universal testing machine with a crosshead speed of 0.5 mm/min. The samples were analyzed with SEM to determinate the failure mode. According to 2-way ANOVA and Tukey's test (α=0.05), the interaction effect (material x luting strategy) was significant (p=0.001). Regardless of the luting strategy, a higher μTBS was obtained with Lava Ultimate and Vita Enamic, which were significantly different from Vita Mark II and Vita Suprinity (p&lt;0.05). For Vita Mark II, Vita Suprinity, Vita Enamic, and Lava Ultimate, the μTBS obtained with RelyX Unicem 2 was not significantly different from that obtained with RelyX ARC or RelyX Ultimate. All groups obtained at least three types of failure. The adhesive strategy with self-adhesive resin cement was comparable to conventional resin cement with total-etch or self-etch adhesive techniques in the bond of novel CAD/CAM materials to dentin.
DOI: 10.6084/m9.figshare.5718367
title: Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

description: ABSTRACT Rhizobium-legume symbioses play relevant roles in agriculture but have not been well studied in Ecuador. The aim of this study was to characterize the genetic and phenotypic diversity of Rhizobium isolates associated with Phaseolus vulgaris from southern Ecuador. Morpho-cultural characterization, biochemical tests and physiological analyses were conducted to authenticate and determine the diversity of bacteria Rhizobium-like isolates. The genetic diversity of the isolates was determined by molecular techniques, which consisted of bacteria DNA extraction and amplification and sequencing of the 16S rRNA gene. The nodulation parameters and nitrogen fixation for P. vulgaris under greenhouse conditions were also assessed to determine the phenotypic diversity among isolates. Furthermore, bacteria indole-acetic-acid production was evaluated by the colorimetric method. Morpho-cultural and biochemical characteristic assessments demonstrated that Rhizobium-like bacteria was associated with the P. vulgaris nodules. The diversity among the isolates, as determined by physiological analyses, revealed the potential of several isolates to grow at different pH values, salinity conditions and temperatures. Partial sequencing of the 16S rRNA gene identified the Rhizobium genus in every sampling site. From a total of 20 aligned sequences, nine species of Rhizobium were identified. Nodule formation and biomass, as well as nitrogen fixation, showed an increase in plant phenotypic parameters, which could be influenced by IAA production, especially for the strains R. mesoamericanum NAM1 and R. leguminosarum bv. viciae COL6. These results demonstrated the efficiency of native symbiotic diazotrophic strains inoculants for legume production. This work can serve as the basis for additional studies of native Rhizobium strains and to help spread the use of biofertilizers in Ecuadorian fields.
DOI: 10.6084/m9.figshare.5718367.v1
title: Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

description: ABSTRACT Rhizobium-legume symbioses play relevant roles in agriculture but have not been well studied in Ecuador. The aim of this study was to characterize the genetic and phenotypic diversity of Rhizobium isolates associated with Phaseolus vulgaris from southern Ecuador. Morpho-cultural characterization, biochemical tests and physiological analyses were conducted to authenticate and determine the diversity of bacteria Rhizobium-like isolates. The genetic diversity of the isolates was determined by molecular techniques, which consisted of bacteria DNA extraction and amplification and sequencing of the 16S rRNA gene. The nodulation parameters and nitrogen fixation for P. vulgaris under greenhouse conditions were also assessed to determine the phenotypic diversity among isolates. Furthermore, bacteria indole-acetic-acid production was evaluated by the colorimetric method. Morpho-cultural and biochemical characteristic assessments demonstrated that Rhizobium-like bacteria was associated with the P. vulgaris nodules. The diversity among the isolates, as determined by physiological analyses, revealed the potential of several isolates to grow at different pH values, salinity conditions and temperatures. Partial sequencing of the 16S rRNA gene identified the Rhizobium genus in every sampling site. From a total of 20 aligned sequences, nine species of Rhizobium were identified. Nodule formation and biomass, as well as nitrogen fixation, showed an increase in plant phenotypic parameters, which could be influenced by IAA production, especially for the strains R. mesoamericanum NAM1 and R. leguminosarum bv. viciae COL6. These results demonstrated the efficiency of native symbiotic diazotrophic strains inoculants for legume production. This work can serve as the basis for additional studies of native Rhizobium strains and to help spread the use of biofertilizers in Ecuadorian fields.
DOI: 10.6084/m9.figshare.5718364
title: The Use of the Internet and Social Networks and the Relationship with Symptoms of Anxiety and Depression among Medical Students

description: ABSTRACT Introduction: The internet is now an essential tool, through which people enjoy easy and rapid access to information and can maintain personal relationships through social networks. However, when its use becomes uncontrolled and maladaptive, what can entail is known as Internet Addiction (IA). In previous studies, several comorbidities associated with this disorder have been investigated, generating knowledge that is important for clinical management. Objective: To investigate the correlation between indicators of internet use and social networks with the presence of symptoms of anxiety and depression. Methods: A descriptive, cross-sectional, quantitative study with convenience sampling, carried out with medical students in 2015. The anxiety and depression symptoms were analyzed through the Beck Anxiety Inventory (BAI) and the Beck Depression Inventory (BDI), respectively. In addition, a questionnaire made by the researchers based on the internet Addiction Test (IAT) was used. Results: Out of the 169 students who participated in the research, 98.8% (167) use the internet and/or social networks on a daily basis. The prevalence of various indications of the harmful use of the internet was evaluated, as well as the participants’ conception of its use. No statistical association was found between the time spent on the internet and the presence of symptoms of anxiety and depression, according to the BAI and BDI scores. However, some statistically significant associations were observed between the results obtained from the BAI and BDI and indicators of the need for internet verification as well as signs of maladaptive use of the internet. Conclusion: The present study ratifies the previous findings in the literature by pointing out that IA is not necessarily related to the time spent on the internet, but rather to a maladaptive pattern of use. The results found here may serve as basis for future interventions in educational institutions that seek to minimize the damage of this disorder, which has become increasingly present.
DOI: 10.6084/m9.figshare.5718361
title: Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

description: Abstract: Endodontic infections are considered to be caused by the presence of various microorganisms within the root canal system. Recognition of this microbiota contributes to the successful treatment of infected root canals. This study investigated the microorganisms associated with primary and secondary endodontic infections via culture methods, biochemical tests, and molecular approaches in an Iranian population. Microbial specimens were collected from 36 patients with primary endodontic infection and 14 patients with a history of root canal therapy. Advanced microbiological culture techniques were used to isolate microbiota; subsequently, biochemical tests and 16S ribosomal RNA gene sequencing were performed to identify the microorganisms. Within the total 218 cultivable isolates, Veillonella parvula (20.6%) was found to occur with the highest frequency in primary endodontic infection, followed by Porphyromonas gingivalis (14.1%), and Aggregatibacter actinomycetemcomitans (9.2%). Enterococcus faecalis (36.6%) was the most predominant microorganism in secondary endodontic infections, followed by Candida albicans, Propionibacterium acnes, and V. parvula with frequencies of 20%, 2%, and 2%, respectively. It was concluded that V. parvula and E. faecalis was most frequently found in primary and secondary endodontic infections, respectively.
DOI: 10.6084/m9.figshare.5718364.v1
title: The Use of the Internet and Social Networks and the Relationship with Symptoms of Anxiety and Depression among Medical Students

description: ABSTRACT Introduction: The internet is now an essential tool, through which people enjoy easy and rapid access to information and can maintain personal relationships through social networks. However, when its use becomes uncontrolled and maladaptive, what can entail is known as Internet Addiction (IA). In previous studies, several comorbidities associated with this disorder have been investigated, generating knowledge that is important for clinical management. Objective: To investigate the correlation between indicators of internet use and social networks with the presence of symptoms of anxiety and depression. Methods: A descriptive, cross-sectional, quantitative study with convenience sampling, carried out with medical students in 2015. The anxiety and depression symptoms were analyzed through the Beck Anxiety Inventory (BAI) and the Beck Depression Inventory (BDI), respectively. In addition, a questionnaire made by the researchers based on the internet Addiction Test (IAT) was used. Results: Out of the 169 students who participated in the research, 98.8% (167) use the internet and/or social networks on a daily basis. The prevalence of various indications of the harmful use of the internet was evaluated, as well as the participants’ conception of its use. No statistical association was found between the time spent on the internet and the presence of symptoms of anxiety and depression, according to the BAI and BDI scores. However, some statistically significant associations were observed between the results obtained from the BAI and BDI and indicators of the need for internet verification as well as signs of maladaptive use of the internet. Conclusion: The present study ratifies the previous findings in the literature by pointing out that IA is not necessarily related to the time spent on the internet, but rather to a maladaptive pattern of use. The results found here may serve as basis for future interventions in educational institutions that seek to minimize the damage of this disorder, which has become increasingly present.
DOI: 10.6084/m9.figshare.5718361.v1
title: Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

description: Abstract: Endodontic infections are considered to be caused by the presence of various microorganisms within the root canal system. Recognition of this microbiota contributes to the successful treatment of infected root canals. This study investigated the microorganisms associated with primary and secondary endodontic infections via culture methods, biochemical tests, and molecular approaches in an Iranian population. Microbial specimens were collected from 36 patients with primary endodontic infection and 14 patients with a history of root canal therapy. Advanced microbiological culture techniques were used to isolate microbiota; subsequently, biochemical tests and 16S ribosomal RNA gene sequencing were performed to identify the microorganisms. Within the total 218 cultivable isolates, Veillonella parvula (20.6%) was found to occur with the highest frequency in primary endodontic infection, followed by Porphyromonas gingivalis (14.1%), and Aggregatibacter actinomycetemcomitans (9.2%). Enterococcus faecalis (36.6%) was the most predominant microorganism in secondary endodontic infections, followed by Candida albicans, Propionibacterium acnes, and V. parvula with frequencies of 20%, 2%, and 2%, respectively. It was concluded that V. parvula and E. faecalis was most frequently found in primary and secondary endodontic infections, respectively.