cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.25442/hku.24637260
title: Supporting data for "Data-efficient Ultrasound Imaging Analysis with Deep Learning"

description: This dataset contains the Supporting data for "Data-efficient Ultrasound Imaging Analysis with Deep Learning". These studies emphasize the importance and demonstrate the feasibility of achieving high deep learning model performance while relaxing data requirements in ultrasound imaging and analysis tasks, including spatial resolution improvement, motion estimation, and tissue segmentation.Chapter 2 introduces the constrained CycleGAN model, which is a novel approach to generate ultrasound sector images with spatial resolution that is more spatially uniform throughout the entire sector field of view. The used datasets is provided in DataSet (Chapter 2).zip.Chapter 3 explores the field of 2D (i.e., lateral and axial) motion estimation in ultrasound imaging, with a special focus on the challenging lateral direction. The used datasets is provided in DataSet (Chapter 3).zip.In Chapter 4, the proposed mask enhanced segmenation model tackles tissue segmentation in dynamic lung ultrasound images. The used datasets is provided in DataSet (Chapter 4).zip.
DOI: 10.25442/hku.24637260.v1
title: Supporting data for "Data-efficient Ultrasound Imaging Analysis with Deep Learning"

description: This dataset contains the Supporting data for "Data-efficient Ultrasound Imaging Analysis with Deep Learning". These studies emphasize the importance and demonstrate the feasibility of achieving high deep learning model performance while relaxing data requirements in ultrasound imaging and analysis tasks, including spatial resolution improvement, motion estimation, and tissue segmentation.Chapter 2 introduces the constrained CycleGAN model, which is a novel approach to generate ultrasound sector images with spatial resolution that is more spatially uniform throughout the entire sector field of view. The used datasets is provided in DataSet (Chapter 2).zip.Chapter 3 explores the field of 2D (i.e., lateral and axial) motion estimation in ultrasound imaging, with a special focus on the challenging lateral direction. The used datasets is provided in DataSet (Chapter 3).zip.In Chapter 4, the proposed mask enhanced segmenation model tackles tissue segmentation in dynamic lung ultrasound images. The used datasets is provided in DataSet (Chapter 4).zip.
DOI: 10.25442/hku.24572863
title: Supporting data for <i>“</i>Metabolic Engineering of 'Last Line Antibiotic' Colistin in <i>Paenibacillus polymyxa</i><i>”</i>

description: Colistin, also known as polymyxin E, is an antibiotic considered as last-line treatment for infections caused by multidrug-resistant gram-negative bacteria. The clinical application of colistin has high standards on its analog ratio and purity, which poses a high challenge to its production and downstream isolation process. Recently, with the advances in synthetic biology techniques, rational metabolic engineering of colistin natural producers is becoming a promising strategy to address this challenge.This study aimed to perform rational metabolic engineering for colistin production in <i>Paenibacillus polymyxa </i>ATCC 842 to construct an ideal host for the efficient production of colistin with a clean background.In the first section, we analyzed the colistin analogs produced by colistin natural<i> </i>producer strain <i>P</i><i>.</i><i> polymyxa</i> ATCC 842 and inactivated its non-essential BGCs. The primary colistin analogs produced by <i>P</i><i>.</i><i> polymyxa</i> ATCC 842 were identified as colistin A and colistin B, and the ratio of the analogs complies with the British Pharmacopoeia and European Pharmacopoeia standards for pharmaceutical colistin. Subsequently, by respective inactivation of 8 non-essential biosynthetic gene clusters (BGCs) via CRISPR/Cas9-based gene editing in the chassis strain, we found that the mutant P3 with inactivation of BGC 4, an NRPS-PKS BGC, could dramatically improve secondary metabolites production, which increased the colistin titer by 79% to 315.4mg/L. Additionally, through multiple inactivation of 7 non-essential BGCs, an engineered strain P14 with a clean metabolic background was generated.In the second section, we further improved the chassis strain's colistin titer by strengthening the precursor supply. Nine promoters in <i>P</i><i>.</i><i> polymyxa</i> ATCC 842 were characterized, and the promoter P<sub>H4</sub> was applied to strengthen the expression of the genes involved in the L-2,4-DABA pathway. By co-overexpression of diaminobutyrate-2-oxoglutarate transaminase <i>ectB</i> and aspartate kinase <i>lysC</i>, an engineered strain P19 with a 649.3 mg/L colistin titer, which was 269% higher than the starting strain, was obtained. Finally, in 5 L bioreactor continuous fermentation, the final colistin titer achieved 1711.7 mg/L.This study represents the pioneering case of metabolic engineering for colistin production in <i>P. polymyxa</i>, its natural producer. We successfully developed an engineered strain, P19, with high colistin production and a clean metabolite background. These findings hold promising implications for advancing the metabolic engineering of NRPS products in non-model Bacillota strains.
DOI: 10.25442/hku.24572863.v1
title: Supporting data for <i>“</i>Metabolic Engineering of 'Last Line Antibiotic' Colistin in <i>Paenibacillus polymyxa</i><i>”</i>

description: Colistin, also known as polymyxin E, is an antibiotic considered as last-line treatment for infections caused by multidrug-resistant gram-negative bacteria. The clinical application of colistin has high standards on its analog ratio and purity, which poses a high challenge to its production and downstream isolation process. Recently, with the advances in synthetic biology techniques, rational metabolic engineering of colistin natural producers is becoming a promising strategy to address this challenge.This study aimed to perform rational metabolic engineering for colistin production in <i>Paenibacillus polymyxa </i>ATCC 842 to construct an ideal host for the efficient production of colistin with a clean background.In the first section, we analyzed the colistin analogs produced by colistin natural<i> </i>producer strain <i>P</i><i>.</i><i> polymyxa</i> ATCC 842 and inactivated its non-essential BGCs. The primary colistin analogs produced by <i>P</i><i>.</i><i> polymyxa</i> ATCC 842 were identified as colistin A and colistin B, and the ratio of the analogs complies with the British Pharmacopoeia and European Pharmacopoeia standards for pharmaceutical colistin. Subsequently, by respective inactivation of 8 non-essential biosynthetic gene clusters (BGCs) via CRISPR/Cas9-based gene editing in the chassis strain, we found that the mutant P3 with inactivation of BGC 4, an NRPS-PKS BGC, could dramatically improve secondary metabolites production, which increased the colistin titer by 79% to 315.4mg/L. Additionally, through multiple inactivation of 7 non-essential BGCs, an engineered strain P14 with a clean metabolic background was generated.In the second section, we further improved the chassis strain's colistin titer by strengthening the precursor supply. Nine promoters in <i>P</i><i>.</i><i> polymyxa</i> ATCC 842 were characterized, and the promoter P<sub>H4</sub> was applied to strengthen the expression of the genes involved in the L-2,4-DABA pathway. By co-overexpression of diaminobutyrate-2-oxoglutarate transaminase <i>ectB</i> and aspartate kinase <i>lysC</i>, an engineered strain P19 with a 649.3 mg/L colistin titer, which was 269% higher than the starting strain, was obtained. Finally, in 5 L bioreactor continuous fermentation, the final colistin titer achieved 1711.7 mg/L.This study represents the pioneering case of metabolic engineering for colistin production in <i>P. polymyxa</i>, its natural producer. We successfully developed an engineered strain, P19, with high colistin production and a clean metabolite background. These findings hold promising implications for advancing the metabolic engineering of NRPS products in non-model Bacillota strains.
DOI: 10.25442/hku.24657234
title: Supporting data for “<b>Inhibition of Polo-like kinase 4 manifests anti-leukemic effects through remodelling histone methylation</b>”

description: This is the dataset for the thesis titled 'Inhibition of Polo-like kinase 4 manifests anti-leukemic effects through remodelling histone methylation'. Analysed and processed data from Western Blot assays (fold change) and flow cytometry assays (MFI) were all included in the excel file. <i>TP53 </i>mutant AML cell lines such as KO52 and THP1 were used, and drug treatment like CFI-400945 (PLK4 inhibitor), GSK591 (PRMT5 inhibitor), EED226 (PRC2 inhibitor) and OSMI-1 (OGTi) were done in this project.
DOI: 10.25442/hku.24657234.v1
title: Supporting data for “<b>Inhibition of Polo-like kinase 4 manifests anti-leukemic effects through remodelling histone methylation</b>”

description: This is the dataset for the thesis titled 'Inhibition of Polo-like kinase 4 manifests anti-leukemic effects through remodelling histone methylation'. Analysed and processed data from Western Blot assays (fold change) and flow cytometry assays (MFI) were all included in the excel file. <i>TP53 </i>mutant AML cell lines such as KO52 and THP1 were used, and drug treatment like CFI-400945 (PLK4 inhibitor), GSK591 (PRMT5 inhibitor), EED226 (PRC2 inhibitor) and OSMI-1 (OGTi) were done in this project.
DOI: 10.25442/hku.24658008
title: Supporting data for “Investigating Eudaimonic Well-Being in the Context of Contemporary China: Theoretical and Empirical Examination”

description: The datafile contains the qualitative interview data for the study evaluating eudaimonic well-being in the context of contemporary China. Part of this project examined spontaneous volunteering during the COVID-19 outbreak. The data file contains the data collected for this purpose, through semi-structured interviews. The datafile consists of eight files, containing four interview recordings and the transcripts of the interview recordings. Interviewees have participated in spontaneous volunteering, and the datafile contains the information of their relevant sharing.
DOI: 10.25442/hku.24658008.v1
title: Supporting data for “Investigating Eudaimonic Well-Being in the Context of Contemporary China: Theoretical and Empirical Examination”

description: The datafile contains the qualitative interview data for the study evaluating eudaimonic well-being in the context of contemporary China. Part of this project examined spontaneous volunteering during the COVID-19 outbreak. The data file contains the data collected for this purpose, through semi-structured interviews. The datafile consists of eight files, containing four interview recordings and the transcripts of the interview recordings. Interviewees have participated in spontaneous volunteering, and the datafile contains the information of their relevant sharing.
DOI: 10.25442/hku.24659409.v1
title: Supporting data for "Integrated Surface Water and Groundwater Modeling of the Baoding Plain in Northern China: Towards Groundwater Sustainability through Managed Aquifer Recharge"

description: The sediment core records (n=736) are with depth from 0.15 m to 608 m.The borehole database consists of a total of 17,314 interpretations of lithology for a total length of 79.1 km of sediments. The original borehole record has roughly more than 300 lithology names and according to the similar grain size and permeability, they were subsequently categorized into clay, silt, fine sand, medium sand, coarse sand, gravel, pebble and gravel, and bedrock with proportions of 49%, 9%, 19%, 9%, 4%, 5%, 3%, 1%, respectively. These data were used for hydrogeological trend analysis and heterogeneous hydrostratigraphic model construction of the Baoding Plain.
DOI: 10.25442/hku.24659409
title: Supporting data for "Integrated Surface Water and Groundwater Modeling of the Baoding Plain in Northern China: Towards Groundwater Sustainability through Managed Aquifer Recharge"

description: The sediment core records (n=736) are with depth from 0.15 m to 608 m.The borehole database consists of a total of 17,314 interpretations of lithology for a total length of 79.1 km of sediments. The original borehole record has roughly more than 300 lithology names and according to the similar grain size and permeability, they were subsequently categorized into clay, silt, fine sand, medium sand, coarse sand, gravel, pebble and gravel, and bedrock with proportions of 49%, 9%, 19%, 9%, 4%, 5%, 3%, 1%, respectively. These data were used for hydrogeological trend analysis and heterogeneous hydrostratigraphic model construction of the Baoding Plain.
DOI: 10.25442/hku.24708171
title: Supporting data for “Harnessing Metal-Organic Frameworks for Precise, Hierarchical, and Reconfigurable Colloidal Self-Assembly”.

description: This main folder includes four sub-folders, each containing supporting data used in Chapters 2, 3, 4, and 5 of the thesis. The data consists of AFM data, Birefriengence data, Confocal data, DLS data, processed images, SEM data, videos, and publications.Additionally, there is a sub-folder called "Notebook" which contains PDF files of scanned lab recordings that provide detailed information about the experiments.Furthermore, the main folder includes a PDF file named "Data Management Plan" that outlines the approach for organizing and handling the data.
DOI: 10.25442/hku.24708171.v1
title: Supporting data for “Harnessing Metal-Organic Frameworks for Precise, Hierarchical, and Reconfigurable Colloidal Self-Assembly”.

description: This main folder includes four sub-folders, each containing supporting data used in Chapters 2, 3, 4, and 5 of the thesis. The data consists of AFM data, Birefriengence data, Confocal data, DLS data, processed images, SEM data, videos, and publications.Additionally, there is a sub-folder called "Notebook" which contains PDF files of scanned lab recordings that provide detailed information about the experiments.Furthermore, the main folder includes a PDF file named "Data Management Plan" that outlines the approach for organizing and handling the data.
DOI: 10.25442/hku.24709860
title: Supporting data for "Ethnosexuality: Exploring Cross-Border Gay Male Desires and Relationships in Japan"

description: This thesis explores cross-border desires and relationships between Japanese and international migrant (white and other Asian) gay men in Japan. By conducting in-depth interviews with twenty local Japanese and twenty international migrant gay men living in the greater Tokyo area and other cities between 2019-2022, this study examines the intersection of sexuality, race, ethnicity, and nationality in their intimate lives. This dataset represents the qualitative data from the research study. To protect the rights and confidentiality of the interviewees, all data has been anonymized. There are two files included in the dataset. One file contains demographic information about all the interviewees, while the other file presents coded interview data along with overarching themes and narratives. Interview data is sensitive, including the sexual and romantic experiences of gay men.
DOI: 10.25442/hku.24709860.v1
title: Supporting data for "Ethnosexuality: Exploring Cross-Border Gay Male Desires and Relationships in Japan"

description: This thesis explores cross-border desires and relationships between Japanese and international migrant (white and other Asian) gay men in Japan. By conducting in-depth interviews with twenty local Japanese and twenty international migrant gay men living in the greater Tokyo area and other cities between 2019-2022, this study examines the intersection of sexuality, race, ethnicity, and nationality in their intimate lives. This dataset represents the qualitative data from the research study. To protect the rights and confidentiality of the interviewees, all data has been anonymized. There are two files included in the dataset. One file contains demographic information about all the interviewees, while the other file presents coded interview data along with overarching themes and narratives. Interview data is sensitive, including the sexual and romantic experiences of gay men.
DOI: 10.25345/c5pg1j00j
title: MassIVE MSV000093665 - Forest Encephalitis Proteomics
title: Proteomic expression profiling of serum of Tick Encephalitis were separated and analyzed by nano-UPLC (EASY-nLC1200) coupled to a Q Exactive HFX Orbitrap instrument (Thermo Fisher Scientific). A total of 24 serum samples comprised 14 patients and 10 healthy controls.
DOI: 10.25442/hku.24754338
title: Supporting data for “BOOSTING PERFORMANCE OF MEMBRANELESS MICROFLUIDIC FUEL CELLS VIA CELL ARCHITECTURE OPTIMIZATION AND FLOW MANAGEMENT”

description: This research focuses on developing high-performance membraneless microfluidic fuel cells (MFCs) via cell architecture designs and flow management. Both self-pumping and pump-assisted MFCs were designed with innovative architectures. Both single-electrolyte and dual-electrolyte MFCs were developed. For each design, the MFC was tested with architecture-sensitive fuel.Fuel-tolerated cathode material was synthesized for the single-electrolyte MFC. HCOOK was dissolved in water to serve as an efficient and safe liquid fuel.To enhance the voltage of a MFC, acid-alkaline dual-electrolyte configuration was adopted. A low-cost hydrogel was sandwiched between the two electrodes and two paper flow channels to restrain the mixing and electrolyte neutralization. The improvement of the gel-aided architecture was proven with H<sub>2</sub>O<sub>2</sub> as both the fuel and oxidant.For pump-assisted MFCs, fuel utilization was frequently sacrificed to achieve high electrochemical performance. To enhance fuel utilization while maintaining high electrochemical performance, the density and viscosity of the co-flow were increased via the addition of suitable polymer additives. The influence of the polymer additives in the electrolytes was investigated with methanol fuel.All the MFCs were optimized through a parametric study. The optimal fuel and electrolyte concentrations were decided. For self-pumping MFCs, their durability and reactivation ability were investigated. For pump-assisted MFCs, optimal and minimum feasible flow rates were explored, and the influence of the flow rate on the fuelThe self-synthesized electrode materials were characterized by XRD, XPS, and Raman spectra, and the electrolytes were studied via FT-IR tests. The morphology of the electrodes was studied by SEM and TEM microscopy. The EPR was employed to assist in the study of the electrochemical disproportionate reactions of H<sub>2</sub>O<sub>2</sub> on each electrode.The overall performances of the MFCs were significantly improved through the innovative designs and flow management in this study. Higher power output is usually provided by pump-assisted MFCs while self-pumping MFCs exhibit better portability and flexibility. The selection of the MFCs is based on the requirements of the applications including the power demands, the dimension restrictions, and operation conditions. In summary, the designs in this study broaden the MFCs' practical applications and pave the way for their future development.<br>
DOI: 10.25442/hku.24754338.v1
title: Supporting data for “BOOSTING PERFORMANCE OF MEMBRANELESS MICROFLUIDIC FUEL CELLS VIA CELL ARCHITECTURE OPTIMIZATION AND FLOW MANAGEMENT”

description: This research focuses on developing high-performance membraneless microfluidic fuel cells (MFCs) via cell architecture designs and flow management. Both self-pumping and pump-assisted MFCs were designed with innovative architectures. Both single-electrolyte and dual-electrolyte MFCs were developed. For each design, the MFC was tested with architecture-sensitive fuel.Fuel-tolerated cathode material was synthesized for the single-electrolyte MFC. HCOOK was dissolved in water to serve as an efficient and safe liquid fuel.To enhance the voltage of a MFC, acid-alkaline dual-electrolyte configuration was adopted. A low-cost hydrogel was sandwiched between the two electrodes and two paper flow channels to restrain the mixing and electrolyte neutralization. The improvement of the gel-aided architecture was proven with H<sub>2</sub>O<sub>2</sub> as both the fuel and oxidant.For pump-assisted MFCs, fuel utilization was frequently sacrificed to achieve high electrochemical performance. To enhance fuel utilization while maintaining high electrochemical performance, the density and viscosity of the co-flow were increased via the addition of suitable polymer additives. The influence of the polymer additives in the electrolytes was investigated with methanol fuel.All the MFCs were optimized through a parametric study. The optimal fuel and electrolyte concentrations were decided. For self-pumping MFCs, their durability and reactivation ability were investigated. For pump-assisted MFCs, optimal and minimum feasible flow rates were explored, and the influence of the flow rate on the fuelThe self-synthesized electrode materials were characterized by XRD, XPS, and Raman spectra, and the electrolytes were studied via FT-IR tests. The morphology of the electrodes was studied by SEM and TEM microscopy. The EPR was employed to assist in the study of the electrochemical disproportionate reactions of H<sub>2</sub>O<sub>2</sub> on each electrode.The overall performances of the MFCs were significantly improved through the innovative designs and flow management in this study. Higher power output is usually provided by pump-assisted MFCs while self-pumping MFCs exhibit better portability and flexibility. The selection of the MFCs is based on the requirements of the applications including the power demands, the dimension restrictions, and operation conditions. In summary, the designs in this study broaden the MFCs' practical applications and pave the way for their future development.<br>
DOI: 10.5281/zenodo.10345805
title: Addressing Climate Change with Behavioral Science:  A Global Intervention Tournament in 63 Countries
DOI: 10.5281/zenodo.10345806
title: Addressing Climate Change with Behavioral Science:  A Global Intervention Tournament in 63 Countries
DOI: 10.34740/kaggle/dsv/7196400
title: WallStreetBets 2022
DOI: 10.6084/m9.figshare.24803481
title: Self-efficacy and birth related PTSD

description: This research evaluated the effect of an internet-based childbirth education program on childbirth self-efficacy antenatally, as well as symptoms of posttraumatic stress disorder (PTSD) and the quality of the mother-infant relationship at six weeks and six months postpartum.
DOI: 10.6084/m9.figshare.24803481.v1
title: Self-efficacy and birth related PTSD

description: This research evaluated the effect of an internet-based childbirth education program on childbirth self-efficacy antenatally, as well as symptoms of posttraumatic stress disorder (PTSD) and the quality of the mother-infant relationship at six weeks and six months postpartum.
DOI: 10.6084/m9.figshare.21298842
title: figure1&S1

description: figure1&S1
DOI: 10.6084/m9.figshare.21298842.v3
title: figure1&S1

description: figure1&S1