cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.14327253
title: Evaluation of the antioxidant potential of whey protein concentrated by ultrafiltration and hydrolyzed by different commercial proteases

description: Abstract The objective of this work was to evaluate the antioxidant and antimicrobial activities of whey protein hydrolysates obtained using different commercial proteases (Alcalase and Flavourzyme) as well as their blend (50:50 ratio). Prior to the enzymatic hydrolysis, the whey was concentrated by ultrafiltration using tubular ceramic membranes. The enzymatic reaction was carried out according to the pH-stat method, in which aliquots were collected with different degrees of hydrolysis (DH) during the protein hydrolysis. The protein hydrolysates were evaluated with respect to the estimated peptides content, antioxidant activity by capture of free radical ABTS+ and the peroxyl radical (Oxygen radical antioxidant capacity - ORAC) methods, electrophoresis and antimicrobial activity. The maximum DH values reached were: 15%, 52% and 63% for Flavourzyme, the blend of enzymes and Alcalase, respectively. The antioxidant activity increased with the DH. The protein hydrolysates obtained using Alcalase presented higher antioxidant activities than the other samples. However, no samples presented antimicrobial activity against Salmonella choleraesuis subsp. Enteritidis (ATCC 13076) or Listeria monocytogenes (ATCC 9117).
DOI: 10.6084/m9.figshare.7773371
title: Determination of metals in fish and shrimp (Farfantepenaeus paulensis) from Patos Lagoon (Pelotas- RS, Brazil) by MIP OES

description: Abstract In the present work, the microwave induced plasma optical emission spectrometry technique was employed to determine the concentrations of Al, Cd, Cu, Hg, Mg, Sb and Zn in fish samples (catfish, mullet, wolf fish, spotted sorubim and croaker) and Al, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Sb, Ti and Zn in shrimp (Farfantepenaeus paulensis) samples. Samples were prepared by acid decomposition with reflux system. For fish analysis, two systems of sample introduction into plasma were compared: conventional nebulization and multimode system. In order to get the best conditions using the multimode system, parameters such as the concentrations of HCl and of reducing agent NaBH4 were investigated. The method accuracy was assessed through analysis of certified reference materials and analyte addition tests. The obtained recoveries were satisfactory for both samples. For fish, the best results were obtained using the multimode system and the maximum concentrations (in mg·kg-1) were: 6.43 to 24.7 for Al; 0.41 to 6.6 for Cu; 168.2 to 211.2 for Mg and 23.1 for Zn. For shrimp samples, the maximum concentrations (in mg·kg -1) were: 92.1 for Al; 1,070 for Ca; 4.57 for Cu; 87.9 for Fe; 451 for Mg; 8.9 for Mn and 29.2 for Zn. All concentrations found for Cd, Cu, Pb and Zn are within the limits permitted by the current legislation in Brazil for fish. The results showed the suitability of the present method using MIP OES technique for multielemental determination in fish and shrimp samples.
DOI: 10.6084/m9.figshare.8092436
title: Improvement of the classification of green asparagus using a Computer Vision System

description: Abstract The aim of this work was to improve the classification of green asparagus in an agro-export company by way of a Computer Vision System (CVS). Thus, an image analysis application was developed in the MATLAB® environment to classify green asparagus according to the absence of white spots and the width of the product. The CVS performance was compared with a manual classification using the error in the classification as the quality indicator; the yield from the raw material (%) and line productivity (kg/h) as the production indicators; and the net present value (USD) and internal rate of return (%) as the economic indicators. The CVS classified the green asparagus with 2% error; improved the yield from the raw material from 43% to 45%, and line productivity from 5 to 10 kg/h; and increased the net present value by 102,609.00 USD, yielding an Internal Rate of Return of 156.3%, much higher than the Opportunity Cost of the Capital (8.6%). Hence the classification of green asparagus by a CVS is an efficient and profitable alternative to manual classification.
DOI: 10.6084/m9.figshare.14327253.v1
title: Evaluation of the antioxidant potential of whey protein concentrated by ultrafiltration and hydrolyzed by different commercial proteases

description: Abstract The objective of this work was to evaluate the antioxidant and antimicrobial activities of whey protein hydrolysates obtained using different commercial proteases (Alcalase and Flavourzyme) as well as their blend (50:50 ratio). Prior to the enzymatic hydrolysis, the whey was concentrated by ultrafiltration using tubular ceramic membranes. The enzymatic reaction was carried out according to the pH-stat method, in which aliquots were collected with different degrees of hydrolysis (DH) during the protein hydrolysis. The protein hydrolysates were evaluated with respect to the estimated peptides content, antioxidant activity by capture of free radical ABTS+ and the peroxyl radical (Oxygen radical antioxidant capacity - ORAC) methods, electrophoresis and antimicrobial activity. The maximum DH values reached were: 15%, 52% and 63% for Flavourzyme, the blend of enzymes and Alcalase, respectively. The antioxidant activity increased with the DH. The protein hydrolysates obtained using Alcalase presented higher antioxidant activities than the other samples. However, no samples presented antimicrobial activity against Salmonella choleraesuis subsp. Enteritidis (ATCC 13076) or Listeria monocytogenes (ATCC 9117).
DOI: 10.6084/m9.figshare.8031197
title: Optimization of a technique to quantify the total phenolic compounds in jambolan ( Syzygium cumini Lamark) pulp

description: Abstract There are various known extraction methods that can be applied to the quantification of phenolic compounds, and thus it is up to the researcher to perfect the process that best applies to the sample in question. Different extraction solvents (liquid-liquid extraction) were compared in order to assess their effectiveness for the analysis of total phenols in jambolan ( Syzygium cumini Lamark) pulp extract using the conventional Folin-Ciocalteau methodology. Three CCRDs (Central Composite Rotational Designs) were carried out to evaluate the factors influencing the extraction process conditions. The variables studied were the solvent concentration (ethanol and methanol, diluted in water) and the mixing time in the compound extraction process. Of the conditions studied, it was shown that a 60% ethanol in water extraction solvent with a mixing time of 10 minutes, in addition to extracting a greater concentration of phenolic compounds, showed the greatest cost benefit advantage, since ethanol is cheaper and less toxic than methanol.
DOI: 10.6084/m9.figshare.7899026.v1
title: Use of sprouts byproduct flour for fresh pasta production

description: Abstract The objective of this study was to elaborate flours of sprouts and sprouts by-product, and to evaluate the potential of sprouts by-products in the fresh pasta prodution. Firstly, alfafa sprouts flours and sprouts by-products flours were produced using conventional drying and freeze-drying. The results showed that the drying method promoted significant differences in moisture, lipids, proteins, total fibers, acidity, water solubility index, water activity and flours coloration. After evaluating results of yield and physical-chemical parameters, sprout by-product flour was selected for fresh pasta production with the following formulations: 5%, 10%, 20% and 30% of sprout by-product flour on wheat flour base (q.s. to 100%). Physical-chemical analysis and technological properties were also performed in all elaborated pastas. Considering the developed pastas, there was an increase in the ash content with the increase of the sprout by-product flour proportion added in the formulation. The technological properties analysis showed an increase in the cooking time and loss of soluble solids in the cooking water with the increase of the sprout by-product flour proportion; the 10% formulation was more adequate compared to the concentration range tested. Therefore, the sprouts by-product demonstrated to be a potential ingredient for paste products, aiming at nutritional and functional improvement.
DOI: 10.6084/m9.figshare.8031197.v1
title: Optimization of a technique to quantify the total phenolic compounds in jambolan ( Syzygium cumini Lamark) pulp

description: Abstract There are various known extraction methods that can be applied to the quantification of phenolic compounds, and thus it is up to the researcher to perfect the process that best applies to the sample in question. Different extraction solvents (liquid-liquid extraction) were compared in order to assess their effectiveness for the analysis of total phenols in jambolan ( Syzygium cumini Lamark) pulp extract using the conventional Folin-Ciocalteau methodology. Three CCRDs (Central Composite Rotational Designs) were carried out to evaluate the factors influencing the extraction process conditions. The variables studied were the solvent concentration (ethanol and methanol, diluted in water) and the mixing time in the compound extraction process. Of the conditions studied, it was shown that a 60% ethanol in water extraction solvent with a mixing time of 10 minutes, in addition to extracting a greater concentration of phenolic compounds, showed the greatest cost benefit advantage, since ethanol is cheaper and less toxic than methanol.
DOI: 10.6084/m9.figshare.8162249
title: Sanitizing cinnamaldehyde solutions against Pseudomonas aeruginosa biofilms formed on stainless steel surfaces

description: Abstract Pseudomonas aeruginosa is an opportunistic pathogen capable of forming biofilms of great relevance to medical microbiology and the food industry. Essential oil (EOs) constituents have been researched as new strategies for the control of biofilms. In the present study cinnamaldehyde was shown to be an effective EO against the planktonic cells of P. aeruginosa strains (p &lt; 0.05). Thus it was used to prepare sanitizing solutions, which were tested against P. aeruginosa biofilms formed on stainless steel surfaces. Cinnamaldehyde showed antibacterial activity against sessile P. aeruginosa cells (p &lt; 0.05). A 100% (5.87 log-reduction) elimination of viable bacterial cells was obtained after treatment with a 6xMIC (0.06% Minimal Inhibitory Concentration) solution for the strain ATCC 9027. Sanitizing cinnamaldehyde solutions can be used as new alternatives to control such microbial sessile communities in food industries.
DOI: 10.6084/m9.figshare.8162249.v1
title: Sanitizing cinnamaldehyde solutions against Pseudomonas aeruginosa biofilms formed on stainless steel surfaces

description: Abstract Pseudomonas aeruginosa is an opportunistic pathogen capable of forming biofilms of great relevance to medical microbiology and the food industry. Essential oil (EOs) constituents have been researched as new strategies for the control of biofilms. In the present study cinnamaldehyde was shown to be an effective EO against the planktonic cells of P. aeruginosa strains (p &lt; 0.05). Thus it was used to prepare sanitizing solutions, which were tested against P. aeruginosa biofilms formed on stainless steel surfaces. Cinnamaldehyde showed antibacterial activity against sessile P. aeruginosa cells (p &lt; 0.05). A 100% (5.87 log-reduction) elimination of viable bacterial cells was obtained after treatment with a 6xMIC (0.06% Minimal Inhibitory Concentration) solution for the strain ATCC 9027. Sanitizing cinnamaldehyde solutions can be used as new alternatives to control such microbial sessile communities in food industries.
DOI: 10.6084/m9.figshare.9871016.v1
title: Phenolic compound, nutritional and antioxidant profile of pollen collected by the genus melipona in North Eastern Brazil

description: Abstract The pollen collected by eusocial bees is often reported as being healthy food due to its important nutritional and therapeutic properties. However, studies reporting such properties are rare, especially for pollen collected by the genus Melipona in northeastern Brazil, which is the focus of this research. Pollen from seven species of stingless bees was analysed for its nutritional composition (sugar, lipid, protein and amino acids). The phenolic compound profile was described based on fourteen phenolic compounds (apigenin, kaempferol, luteolin, naringin, rutin, gallic acid, ferulic acid, caffeic acid, p-coumaric acid, chlorogenic acid, abscisic acid, protocatechuic acid, vanillic acid and trans-cinnamic acid). The antioxidant property was analysed by quantifying of total phenolic compounds, total flavonoids and DPPH. Chromatographic methods were used to identify and quantify the phenolic compounds and amino acids. The pollen samples from the bees under study showed good concentrations of proteins and amino acids and good antioxidant potential. The phenolic compounds luteolin, trans-cinnamic acid and apigenin were identified and described in pollen for the first time. Of the amino acids analysed, asparigine, glutamic acid, leucine and proline showed the highest concentrations. The research related to the theme showed that this is one of the first studies to identify and quantify the phenolic compounds and amino acids in stingless bee pollen, reflecting its importance in therapeutic use and as a food supplement.
DOI: 10.6084/m9.figshare.9871016
title: Phenolic compound, nutritional and antioxidant profile of pollen collected by the genus melipona in North Eastern Brazil

description: Abstract The pollen collected by eusocial bees is often reported as being healthy food due to its important nutritional and therapeutic properties. However, studies reporting such properties are rare, especially for pollen collected by the genus Melipona in northeastern Brazil, which is the focus of this research. Pollen from seven species of stingless bees was analysed for its nutritional composition (sugar, lipid, protein and amino acids). The phenolic compound profile was described based on fourteen phenolic compounds (apigenin, kaempferol, luteolin, naringin, rutin, gallic acid, ferulic acid, caffeic acid, p-coumaric acid, chlorogenic acid, abscisic acid, protocatechuic acid, vanillic acid and trans-cinnamic acid). The antioxidant property was analysed by quantifying of total phenolic compounds, total flavonoids and DPPH. Chromatographic methods were used to identify and quantify the phenolic compounds and amino acids. The pollen samples from the bees under study showed good concentrations of proteins and amino acids and good antioxidant potential. The phenolic compounds luteolin, trans-cinnamic acid and apigenin were identified and described in pollen for the first time. Of the amino acids analysed, asparigine, glutamic acid, leucine and proline showed the highest concentrations. The research related to the theme showed that this is one of the first studies to identify and quantify the phenolic compounds and amino acids in stingless bee pollen, reflecting its importance in therapeutic use and as a food supplement.
DOI: 10.6084/m9.figshare.9871010.v1
title: Use of response surface methodology in optimization of phenolic compounds extraction from the shell of Hymenaea courbaril L. (Jatobá) fruits

description: Abstract The food and medicinal habits of Amazon people are rich in fruits considered as exotic or commercially underutilized, some of which generate a considerable amount of residue. The Hymenaea courbaril L. (Jatobá) fruit shell was submitted to a simultaneous extraction of total phenolic compounds, optimized by response surface methodology. Preliminary experiments, investigating sample-solvent ratio (g/mL), time (minutes) and extraction temperature (ºC), indicate the limits for experimental region design, which was monitored by rotational central composite design consisting of 19 runs. The experimental results fitted well to the second order polynomial model generated, which was able to significantly describe the observed variability for response variables. The optimized conditions were 60.6% ethanol, 64.18 ° C, 71.93 minutes and the model presented predicted values of 35.265 ± 2.22 mg gallic acid equivalent / g dry matter and 1.210 ± 0.16 mg rutin equivalent / g dry matter, for total phenolic compounds and total flavonoids, respectively. The experimental observed values, 33.234 ± 1.66 mg gallic acid equivalent / g dry mater and 1.216 ± 0.008 mg rutin equivalent / g dry matter, for total phenolic compounds and total flavonoids respectively, agreed with those predicted within a 95% confidence interval. These results confirm the predictive capacity and the adequacy of response surface methodology in optimizing simultaneous extraction of total phenolic compounds and total flavonoids from shell of jatobá fruits.
DOI: 10.6084/m9.figshare.9871010
title: Use of response surface methodology in optimization of phenolic compounds extraction from the shell of Hymenaea courbaril L. (Jatobá) fruits

description: Abstract The food and medicinal habits of Amazon people are rich in fruits considered as exotic or commercially underutilized, some of which generate a considerable amount of residue. The Hymenaea courbaril L. (Jatobá) fruit shell was submitted to a simultaneous extraction of total phenolic compounds, optimized by response surface methodology. Preliminary experiments, investigating sample-solvent ratio (g/mL), time (minutes) and extraction temperature (ºC), indicate the limits for experimental region design, which was monitored by rotational central composite design consisting of 19 runs. The experimental results fitted well to the second order polynomial model generated, which was able to significantly describe the observed variability for response variables. The optimized conditions were 60.6% ethanol, 64.18 ° C, 71.93 minutes and the model presented predicted values of 35.265 ± 2.22 mg gallic acid equivalent / g dry matter and 1.210 ± 0.16 mg rutin equivalent / g dry matter, for total phenolic compounds and total flavonoids, respectively. The experimental observed values, 33.234 ± 1.66 mg gallic acid equivalent / g dry mater and 1.216 ± 0.008 mg rutin equivalent / g dry matter, for total phenolic compounds and total flavonoids respectively, agreed with those predicted within a 95% confidence interval. These results confirm the predictive capacity and the adequacy of response surface methodology in optimizing simultaneous extraction of total phenolic compounds and total flavonoids from shell of jatobá fruits.
DOI: 10.6084/m9.figshare.9957179
title: Food safety: seasonal influence on parasitic contamination of lettuce (Lactuca sativa L.) commercialized in street markets in Belém, Pará, Brazil

description: Abstract The consumption of fresh vegetables in Brazil and in the world has been increasing significantly. Of these foods, lettuce (Lactuca sativa L.) is the vegetable most consumed in Brazil, since it is suitable for several types of diet and presents a low caloric value. Nevertheless, the preference for this type of food and its benefits may expose consumers to the risk of contracting infections due to various pathogens. The objective of the present study was to evaluate the influence of seasonality on the parasitic contamination of lettuces commercialized in four street markets in the city of Belém-PA, Brazil. One hundred and twenty lettuce specimens were obtained from municipal street markets in Belém during the dry and rainy seasons, and then processed and analysed individually by the spontaneous sedimentation method. The level of contamination of the vegetables was found to be similar in the different seasonal periods, being 36.7% during the dry season and 38.7% in the rainy season. Mixed parasitic contamination, detected especially in the rainy season, suggests that the rainfall indices may influence the increase in the number of parasite species potentially affecting the population. The results showed elevated contamination by parasitic agents in all the street markets that commercialized the vegetable. The main parasitic species detected were Ascaris sp., Ancylostoma sp., Strongyloides sp., Enterobius vermicularis, Taenia sp., Toxoplasma gondii oocyst, and other dirt. The study identified a moderate rate of contamination by helminths and protozoans in the lettuces sold in the street markets of the city of Belém, showing evidence of the continuous risk of parasitic infections to humans from the consumption of raw vegetables, which justifies the implementation of measures that allow for an improvement in the hygienic-sanitary quality.
DOI: 10.6084/m9.figshare.9957179.v1
title: Food safety: seasonal influence on parasitic contamination of lettuce (Lactuca sativa L.) commercialized in street markets in Belém, Pará, Brazil

description: Abstract The consumption of fresh vegetables in Brazil and in the world has been increasing significantly. Of these foods, lettuce (Lactuca sativa L.) is the vegetable most consumed in Brazil, since it is suitable for several types of diet and presents a low caloric value. Nevertheless, the preference for this type of food and its benefits may expose consumers to the risk of contracting infections due to various pathogens. The objective of the present study was to evaluate the influence of seasonality on the parasitic contamination of lettuces commercialized in four street markets in the city of Belém-PA, Brazil. One hundred and twenty lettuce specimens were obtained from municipal street markets in Belém during the dry and rainy seasons, and then processed and analysed individually by the spontaneous sedimentation method. The level of contamination of the vegetables was found to be similar in the different seasonal periods, being 36.7% during the dry season and 38.7% in the rainy season. Mixed parasitic contamination, detected especially in the rainy season, suggests that the rainfall indices may influence the increase in the number of parasite species potentially affecting the population. The results showed elevated contamination by parasitic agents in all the street markets that commercialized the vegetable. The main parasitic species detected were Ascaris sp., Ancylostoma sp., Strongyloides sp., Enterobius vermicularis, Taenia sp., Toxoplasma gondii oocyst, and other dirt. The study identified a moderate rate of contamination by helminths and protozoans in the lettuces sold in the street markets of the city of Belém, showing evidence of the continuous risk of parasitic infections to humans from the consumption of raw vegetables, which justifies the implementation of measures that allow for an improvement in the hygienic-sanitary quality.
DOI: 10.6084/m9.figshare.10026113
title: Essential oils in the control of Botrytis cinera: influence on post harvest quality of Rubi grapes

description: Abstract Agrochemicals are used to control diseases in post-harvest grapes, but although efficient, they may cause human health problems. Thus, the objective of the present work was to verify the potential of the volatile compounds present in the essential oils of the fruits pitanga (EOP), guaçatonga (EOG) and melaleuca (EOM) in the control of Botrytis cinerea both in vitro and inoculated in ' Rubi ' grapes, as well as to verify the induction of resistance in these fruits. Thus, B. cinerea mycelial growth, grey mould control in 'Rubi' grapes, post-harvest quality of the grapes and the activities of the enzymes polyphenoloxidase (PPO), phenylalanine ammonium lyase (PAL), glucanase (GLU) and chitinase, were evaluated. According to the results it was observed that the volatile compounds released by the essential oils reduced the rates of mycelial growth of B. cinerea and of the grey mould disease, and the treatments with the volatiles from EOP and EOG activated the resistance induction mechanisms of these fruits. Thus, EOP and OEG release compounds that can act directly on B. cinerea and also induce resistance in the 'Rubi' grapes, reducing the grey mould in these grapes.