cometadata/datacite-titles-descriptions-related-identifiers huggingface dataset:
DOI: 10.6084/m9.figshare.12094875.v1
title: The effects of the Paleolithic Diet on obesity anthropometric measurements

description: Abstract Comparing the effects of Paleolithic Diet (PD) and of a Guidelines Substantiated Diet (GSD) on anthropometric indicators of obese individuals. Randomized clinical trial. Obese patients were divided into two groups based on dietary prescription: PD and GSD. These diets encompassed 82 and 73 patients, respectively, who were followed-up for 60 days. Anthropometric measurements were taken before the beginning of the trial, at the 30th day of it and at the end of the experimental period. At the 60th experimental day, there was 26.8% treatment abandonment by individuals in group PD and 19.2% by the ones in group GSD; there was not difference between groups (p = 0.684). Weight and height measurements were taken in order to determine body mass index (BMI), waist circumference (WC) and hip circumference (HC), which allowed determining the waist/hip ratio (WHR). There was no initial difference between groups in demographic, socioeconomic, exercising and anthropometric variables (p &gt; 0.05). Group PD recorded the highest weight (p = 0.003), BMI (p = 0.002) and WC (p = 0.033) values at the 30th experimental day. Group PD kept the highest weight loss at the 60th day (p &lt; 0,001), as well as the highest BMI (p &lt; 0.001), WC (p = 0.002) and WHR (p = 0.002) reduction. PD is a feasible option to treat obesity, it led to reduced anthropometric markers. Other studies are necessary to assure the safety of its use for longer periods of time.
DOI: 10.6084/m9.figshare.12094866
title: Assessment of the acid phosphatase CP01850 from Corynebacterium pseudotuberculosis in DNA and subunit vaccine formulations against caseous lymphadenitis

description: ABSTRACT The target cp1002_RS01850 from Corynebacterium pseudotuberculosis was used to construct a DNA and recombinant subunit vaccine against caseous lymphadenitis. Recombinant protein rCP01850 was expressed in Escherichia coli using pAE vector, and DNA vaccine was engineered with pTARGET vector. BALB/c mice were divided in five groups containing eight animals each, inoculated with: pTARGET/cp01850 as DNA vaccine (G1); rCP01850 plus Al (OH)3 as recombinant subunit vaccine (G2); pTARGET/cp01850 and a boost with rCP01850 plus Al (OH)3 (G3); pTARGET (G4); or Al (OH)3 (G5). Mice were inoculated and blood samples were collected on days 0, 21, and 42 for the analysis of total IgG, IgG1 and IgG2a by ELISA. In each group, five animals were challenged with Mic-6 C. pseudotuberculosis strain, and three were used for cytokine quantification by qPCR. Although no group has been protected by vaccines against lethal challenge, G2 showed an increase in the survival rate after challenge. Significantly higher levels of IL-4, IL-12, IFN-γ, total IgG, IgG1 and IgG2a were also detected for G2, evidencing a mixed Th1/Th2 immunological profile. In conclusion, despite no protection level provided by different vaccinal strategies using cp1002_RS01850 from C. pseudotuberculosis, G2 developed a Th1/Th2 immune response with an increase in survival rate.
DOI: 10.6084/m9.figshare.12094857
title: [Effect of maternal hypothyroidism on the spatiotemporal expression of immunological mediators and population of Natural Killers cells in decidua and metrial gland of rats]

description: ABSTRACT The goal of this study was to evaluate the effect of maternal hypothyroidism on the spatiotemporal expression of immunological mediators and population of Natural Killers cells in decidua and metrial gland of rats. Interferon gamma (IFNγ), migration inhibitory factor (MIF), interleukin 15 (IL15), inducible nitric oxide synthase (iNOS), and DBA-Lectin labeling for evidence of uNK DBA+ cells in decidua and genetic expression of Ifnγ and iNos by real-time RT-PCR were evaluated. Hypothyroidism increased protein expression of iNOS at 7 days, IL15 and MIF at 10 and 12 days, IFNγ and MIF at 14 DG in the decidua and/or metrial gland and the gene transcripts for iNOS at 12 and 19 days and for Inf at 14 DG. In addition, hypothyroidism reduced the protein expression of MIF and DBA-Lectin at 7 days, DBA-Lectin at 10 and 14 DG, IFNγ at 12 days, and the gene transcript to Ifnγ at 10 and 19 DGs. Hypothyroidism also reduced the protein expression of iNOS at 12, 14 and 19 DG and reduced its gene transcripts at 10 and 14 DGs. It is concluded that hypothyroidism compromises the immunology profile at the maternal-fetal interface throughout pregnancy, particularly by reducing the anti-inflammatory factor iNOS and population of uNK DBA+ cells.
DOI: 10.6084/m9.figshare.12094866.v1
title: Assessment of the acid phosphatase CP01850 from Corynebacterium pseudotuberculosis in DNA and subunit vaccine formulations against caseous lymphadenitis

description: ABSTRACT The target cp1002_RS01850 from Corynebacterium pseudotuberculosis was used to construct a DNA and recombinant subunit vaccine against caseous lymphadenitis. Recombinant protein rCP01850 was expressed in Escherichia coli using pAE vector, and DNA vaccine was engineered with pTARGET vector. BALB/c mice were divided in five groups containing eight animals each, inoculated with: pTARGET/cp01850 as DNA vaccine (G1); rCP01850 plus Al (OH)3 as recombinant subunit vaccine (G2); pTARGET/cp01850 and a boost with rCP01850 plus Al (OH)3 (G3); pTARGET (G4); or Al (OH)3 (G5). Mice were inoculated and blood samples were collected on days 0, 21, and 42 for the analysis of total IgG, IgG1 and IgG2a by ELISA. In each group, five animals were challenged with Mic-6 C. pseudotuberculosis strain, and three were used for cytokine quantification by qPCR. Although no group has been protected by vaccines against lethal challenge, G2 showed an increase in the survival rate after challenge. Significantly higher levels of IL-4, IL-12, IFN-γ, total IgG, IgG1 and IgG2a were also detected for G2, evidencing a mixed Th1/Th2 immunological profile. In conclusion, despite no protection level provided by different vaccinal strategies using cp1002_RS01850 from C. pseudotuberculosis, G2 developed a Th1/Th2 immune response with an increase in survival rate.
DOI: 10.6084/m9.figshare.12094857.v1
title: [Effect of maternal hypothyroidism on the spatiotemporal expression of immunological mediators and population of Natural Killers cells in decidua and metrial gland of rats]

description: ABSTRACT The goal of this study was to evaluate the effect of maternal hypothyroidism on the spatiotemporal expression of immunological mediators and population of Natural Killers cells in decidua and metrial gland of rats. Interferon gamma (IFNγ), migration inhibitory factor (MIF), interleukin 15 (IL15), inducible nitric oxide synthase (iNOS), and DBA-Lectin labeling for evidence of uNK DBA+ cells in decidua and genetic expression of Ifnγ and iNos by real-time RT-PCR were evaluated. Hypothyroidism increased protein expression of iNOS at 7 days, IL15 and MIF at 10 and 12 days, IFNγ and MIF at 14 DG in the decidua and/or metrial gland and the gene transcripts for iNOS at 12 and 19 days and for Inf at 14 DG. In addition, hypothyroidism reduced the protein expression of MIF and DBA-Lectin at 7 days, DBA-Lectin at 10 and 14 DG, IFNγ at 12 days, and the gene transcript to Ifnγ at 10 and 19 DGs. Hypothyroidism also reduced the protein expression of iNOS at 12, 14 and 19 DG and reduced its gene transcripts at 10 and 14 DGs. It is concluded that hypothyroidism compromises the immunology profile at the maternal-fetal interface throughout pregnancy, particularly by reducing the anti-inflammatory factor iNOS and population of uNK DBA+ cells.
DOI: 10.6084/m9.figshare.12094845
title: In vitro evaluation of antagonists against soursop fruit pathogens (Annona muricata L.) in Nayarit, Mexico

description: Abstract In Nayarit, Mexico, soursop production is affected by the pathogens of Pestalotiopsis sp. and Colletotrichum gloeosporioides that cause dry rot and Lasiodiplodia pseudotheobromae soft rot. In the present study, the in vitro antagonism of three species of Streptomyces, three of Trichoderma and three of Bacillus were evaluated. The Trichoderma strains showed different inhibition in the pathogens evaluated, Trichoderma longibrachiatum showed the highest PIRG in vitro, which was 60.45, 59.33 and 57.9 when confronted against C. gloeosporioides, Pestalotiopsis sp., and L. pseudotheobromae, respectively. The bacteria showed lower PIRG than the fungi. B. methylotrophicus exhibited the highest capacity of inhibition of the experiment with 58.22 % of PIRG against Pestalotiopsis sp. The Streptomyces strains were the most effective and could be studied in future researches on the biological control of fruit pathogens, since they exerted 100 % inhibition except S. viridochromogenes that presented a percentage of inhibition of radial growth (PIRG) of 88.3 % against Lasiodiplodia pseudotheobromae.
DOI: 10.6084/m9.figshare.12094848
title: Effect of Nickel Powder Buffering Layer on Microstructure and Hardness Properties of High Carbon Steel / Stainless Steel Arc Stud Welding

description: Abstract In this study, arc stud welding process was employed for welding AISI 316 stainless steel studs to AISI 1060 high carbon steel plates. A disc of Ni powder prepared and used as a buffering layer to enhance the properties of welding area. Optical and scanning electron microscopy were used to examine the microstructure. Energy-Dispersive X-ray (EDX) and X-Ray Diffraction (XRD) tests were performed to analyse and identify elements and phases, respectively in the weld region. The results observed that Ni powder prevented the direct contact between the dissimilar base metals. Existing of Ni altered the microstructure of the weld zone and encouraged dendritic type over cellular. Hardness reduced in the weld region from 600 HV to 200 HV due to the effect of Ni powder which prevented the formation of brittle Fe-Cr phase.
DOI: 10.6084/m9.figshare.12094836
title: Susceptibility of four passion fruit species to the reniform nematode

description: Abstract Reniform nematode (Rotylenchulus reniformis) is a major pest of sour-passion fruit (Passiflora edulis) in Brazil, and few management methods are currently available, mainly due to the lack of host-plant resistance. Other species of passion fruit species are also commercially available in Brazil, which could be a source of resistance. Cultivars of three species (P. setacea ‘BRS-Pérola-do-Cerrado’, P. cincinnata ‘BRS-Sertão-Forte’, and P. alata ‘BRS-Mel-do-Cerrado’), and one susceptible cultivar (P. edulis BRS-Gigante-Amarelo) were evaluated for reniform nematode susceptibility in two greenhouse trials. Results showed that the cultivars were susceptible. Therefore, reniform nematode management in passion fruit must be rely on a combination of nematode-free seedlings and nematode-free fields.
DOI: 10.6084/m9.figshare.12094845.v1
title: In vitro evaluation of antagonists against soursop fruit pathogens (Annona muricata L.) in Nayarit, Mexico

description: Abstract In Nayarit, Mexico, soursop production is affected by the pathogens of Pestalotiopsis sp. and Colletotrichum gloeosporioides that cause dry rot and Lasiodiplodia pseudotheobromae soft rot. In the present study, the in vitro antagonism of three species of Streptomyces, three of Trichoderma and three of Bacillus were evaluated. The Trichoderma strains showed different inhibition in the pathogens evaluated, Trichoderma longibrachiatum showed the highest PIRG in vitro, which was 60.45, 59.33 and 57.9 when confronted against C. gloeosporioides, Pestalotiopsis sp., and L. pseudotheobromae, respectively. The bacteria showed lower PIRG than the fungi. B. methylotrophicus exhibited the highest capacity of inhibition of the experiment with 58.22 % of PIRG against Pestalotiopsis sp. The Streptomyces strains were the most effective and could be studied in future researches on the biological control of fruit pathogens, since they exerted 100 % inhibition except S. viridochromogenes that presented a percentage of inhibition of radial growth (PIRG) of 88.3 % against Lasiodiplodia pseudotheobromae.
DOI: 10.6084/m9.figshare.12094836.v1
title: Susceptibility of four passion fruit species to the reniform nematode

description: Abstract Reniform nematode (Rotylenchulus reniformis) is a major pest of sour-passion fruit (Passiflora edulis) in Brazil, and few management methods are currently available, mainly due to the lack of host-plant resistance. Other species of passion fruit species are also commercially available in Brazil, which could be a source of resistance. Cultivars of three species (P. setacea ‘BRS-Pérola-do-Cerrado’, P. cincinnata ‘BRS-Sertão-Forte’, and P. alata ‘BRS-Mel-do-Cerrado’), and one susceptible cultivar (P. edulis BRS-Gigante-Amarelo) were evaluated for reniform nematode susceptibility in two greenhouse trials. Results showed that the cultivars were susceptible. Therefore, reniform nematode management in passion fruit must be rely on a combination of nematode-free seedlings and nematode-free fields.
DOI: 10.6084/m9.figshare.12094848.v1
title: Effect of Nickel Powder Buffering Layer on Microstructure and Hardness Properties of High Carbon Steel / Stainless Steel Arc Stud Welding

description: Abstract In this study, arc stud welding process was employed for welding AISI 316 stainless steel studs to AISI 1060 high carbon steel plates. A disc of Ni powder prepared and used as a buffering layer to enhance the properties of welding area. Optical and scanning electron microscopy were used to examine the microstructure. Energy-Dispersive X-ray (EDX) and X-Ray Diffraction (XRD) tests were performed to analyse and identify elements and phases, respectively in the weld region. The results observed that Ni powder prevented the direct contact between the dissimilar base metals. Existing of Ni altered the microstructure of the weld zone and encouraged dendritic type over cellular. Hardness reduced in the weld region from 600 HV to 200 HV due to the effect of Ni powder which prevented the formation of brittle Fe-Cr phase.
DOI: 10.6084/m9.figshare.12094818
title: TNF-alpha inhibitor adalimumab attenuates endotoxin induced cardiac damage in rats

description: Abstract Purpose To investigate the effects of adalimumab pretreatment on the lipopolysaccharide-mediated myocardial injury. Methods Twenty-eight Wistar rats were randomized into four groups (n=7). Control (C) group animals were injected once a day with intraperitoneal (i.p) 0.9 % saline for two days. In the Adalimumab (Ada) group, adalimumab was injected at a dose of 10 mg/kg/ day (i.p) for two days. Lipopolysaccharide (Lps) group rats were injected with a dose of 5 mg/kg (i.p) lipopolysaccharide. Lipopolysaccharide + Adalimumab (Lps+Ada) group rats received adalimumab before the administration of lipopolysaccharide. The animals were sacrificed 24 h after the last injection and blood samples were obtained for determination of biochemical cardiac injury markers and circulating levels of TNF-α and interleukin-6 (IL-6). Hearts were harvested for histological examination. Results Endotoxin exposure resulted in significant increases in serum cardiac injury markers, serum cytokines and histological myocardial injury scores in the Lps group. The levels of circulating cytokines, cardiac injury markers and histological injury scores for myocardial necrosis, perivascular cell infiltration, and inflammation were significantly reduced in Lps+Ada as compared to Lps group (p&lt;0.05). Conclusions Adalimumab pretreatment reduces endotoxin-induced myocardial damage in rats. This beneficial effect is thought to be related to the reduction of cytokine release.
DOI: 10.6084/m9.figshare.12094818.v1
title: TNF-alpha inhibitor adalimumab attenuates endotoxin induced cardiac damage in rats

description: Abstract Purpose To investigate the effects of adalimumab pretreatment on the lipopolysaccharide-mediated myocardial injury. Methods Twenty-eight Wistar rats were randomized into four groups (n=7). Control (C) group animals were injected once a day with intraperitoneal (i.p) 0.9 % saline for two days. In the Adalimumab (Ada) group, adalimumab was injected at a dose of 10 mg/kg/ day (i.p) for two days. Lipopolysaccharide (Lps) group rats were injected with a dose of 5 mg/kg (i.p) lipopolysaccharide. Lipopolysaccharide + Adalimumab (Lps+Ada) group rats received adalimumab before the administration of lipopolysaccharide. The animals were sacrificed 24 h after the last injection and blood samples were obtained for determination of biochemical cardiac injury markers and circulating levels of TNF-α and interleukin-6 (IL-6). Hearts were harvested for histological examination. Results Endotoxin exposure resulted in significant increases in serum cardiac injury markers, serum cytokines and histological myocardial injury scores in the Lps group. The levels of circulating cytokines, cardiac injury markers and histological injury scores for myocardial necrosis, perivascular cell infiltration, and inflammation were significantly reduced in Lps+Ada as compared to Lps group (p&lt;0.05). Conclusions Adalimumab pretreatment reduces endotoxin-induced myocardial damage in rats. This beneficial effect is thought to be related to the reduction of cytokine release.
DOI: 10.6084/m9.figshare.12094821
title: Impact of temporomandibular disorders on quality of life

description: Abstract Introduction: Temporomandibular dysfunction (TMD) may have a major impact on quality of life. Objective: Thus, this integrative review assessed the impact of TMD on quality of life. Method: An electronic and manual search was conducted to identify studies that evaluated the impact of TMD on an individual’s quality of life. After the inclusion and exclusion criteria were met, seven articles were included and evaluated according to the quality of evidence using the Newcastle-Ottawa assessment. Results: The selected studies used different instruments to diagnose temporomandibular joint disorders and measure the quality of life. Only three studies used the RDC/TMD. As for quality of life, the most used instrument was the SF-36, followed by Br-MPQ and WHOQOL-Bref. Conclusion: The findings show that there is a negative impact of temporomandibular dysfunction on quality of life, especially regarding its severity. However, further studies are needed to confirm these results.
DOI: 10.6084/m9.figshare.12094812
title: Extraction, characterization and microencapsulation of isoflavones from soybean molasses

description: ABSTRACT: This study aimed to extract isoflavones from soybean molasses with different solvents, furthermore, the selected extract, which showed overall desirable characteristics was selected to evaluate the potentials of different encapsulating agents. The encapsulating agents employed for the study included 18% Maltodextrin DE20 (T1), 18% Hi-maize (T2), and a mixture of equal proportions of 9% Maltodextrin DE20 and 9% of Hi-maize (T3). Solvents such as 80% ethanol and methanol, and grain alcohol in varying different concentrations of 50 and 80% were used for the studies. The best solvent for the extraction of phenolics and total isoflavones was 50% cereal alcohol, this extract also presented higher antioxidant activity. Evaluation of the encapsulating agents revealed that 18% Hi-maize with inlet air of 130 °C was best suited for the encapsulation of isoflavones. The ORAC method showed that microcapsules with the 18% Hi-maize encapsulating agent also had higher antioxidant activity.
DOI: 10.6084/m9.figshare.12094821.v1
title: Impact of temporomandibular disorders on quality of life

description: Abstract Introduction: Temporomandibular dysfunction (TMD) may have a major impact on quality of life. Objective: Thus, this integrative review assessed the impact of TMD on quality of life. Method: An electronic and manual search was conducted to identify studies that evaluated the impact of TMD on an individual’s quality of life. After the inclusion and exclusion criteria were met, seven articles were included and evaluated according to the quality of evidence using the Newcastle-Ottawa assessment. Results: The selected studies used different instruments to diagnose temporomandibular joint disorders and measure the quality of life. Only three studies used the RDC/TMD. As for quality of life, the most used instrument was the SF-36, followed by Br-MPQ and WHOQOL-Bref. Conclusion: The findings show that there is a negative impact of temporomandibular dysfunction on quality of life, especially regarding its severity. However, further studies are needed to confirm these results.
DOI: 10.6084/m9.figshare.12094809
title: Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry

description: ABSTRACT: The objective of this study was to evaluate the effect of the initial microbial load, temperature and contact time on the biofilm formation of Alicyclobacillus acidoterrestris on stainless steel and natural food-grade rubber using orange juice as culture medium. The low initial load of A. acidoterrestris (2 log CFU/mL) led to biofilm formation on the stainless steel surface after 48 h of contact at 28 ºC and after 24 h at 45 ºC, and on natural food-grade rubber surface after 48 h of contact at both temperatures. The high initial microbial load (5 log CFU/mL) led to biofilm formation on stainless steel after 4 h of contact at 28 °C and 45 °C, while biofilm was formed on natural food-grade rubber after 8 h of contact at 28 °C and 4 h at 45 °C. The microbial load also affected the presence of spores in biofilm, which was observed on both surfaces only at high initial loads of A. acidoterrestris.
DOI: 10.6084/m9.figshare.12094803
title: Evidence on the use of internet for businesses by MSEs in a Developing Country. The Indonesian case

description: Abstract This study aims to examine the development of Indonesian micro and small enterprises (MSEs) in utilizing the internet for their businesses. This is a descriptive study in nature, which analyses secondary data on MSMEs. It also reviews key literature on the use of ICT by MSMEs in developing countries. It shows that MSMEs, dominated by MSEs, have been the main player in Indonesian domestic economic activities as they accounted for more than 90 per cent of all firms and contributed to more than 50 per cent of gross domestic product (GDP). But, only a small fraction of total MSEs in Indonesia that utilize the internet for business, and the intensity of internet usage by MSEs varies by province and type of business. This is the first macro-level study ever conducted in Indonesia on the use of internet by MSEs based on national data from 2016 Economic Census conducted. Thus, findings of this study may add new empirical evidence to the literature on the utilization of ICT by MSMEs in developing countries.
DOI: 10.6084/m9.figshare.12094812.v1
title: Extraction, characterization and microencapsulation of isoflavones from soybean molasses

description: ABSTRACT: This study aimed to extract isoflavones from soybean molasses with different solvents, furthermore, the selected extract, which showed overall desirable characteristics was selected to evaluate the potentials of different encapsulating agents. The encapsulating agents employed for the study included 18% Maltodextrin DE20 (T1), 18% Hi-maize (T2), and a mixture of equal proportions of 9% Maltodextrin DE20 and 9% of Hi-maize (T3). Solvents such as 80% ethanol and methanol, and grain alcohol in varying different concentrations of 50 and 80% were used for the studies. The best solvent for the extraction of phenolics and total isoflavones was 50% cereal alcohol, this extract also presented higher antioxidant activity. Evaluation of the encapsulating agents revealed that 18% Hi-maize with inlet air of 130 °C was best suited for the encapsulation of isoflavones. The ORAC method showed that microcapsules with the 18% Hi-maize encapsulating agent also had higher antioxidant activity.